LOCUS       DASYNS010000001        16640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5304350, whole genome shotgun sequence.
ACCESSION   DASYNS010000001 DASYNS010000000
VERSION     DASYNS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 16640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16640
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5304350"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..1015)
                     /locus_tag="VGP50_00005"
     CDS             complement(359..1015)
                     /locus_tag="VGP50_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV8025772.1"
                     /translation="MRAKKRREAGPPKRAAKVSSAATPSKEAEVAAVARTVSENLRRL
                     RVGRELSLEQLSKEAGVSRGMLRQIELARSVPTIGLLWRVARALRVPFAALISDESQS
                     GTVLLPGANAKILSSVDGGFTSRALFPFDAERRVEFYKLTLGPGTEEIAAPHAPGTKE
                     NLVVATGQVEIVVAGVSHVLRSDDAIMFAADVPHAYRNRGSKQAILYLVMTYIEAVGA
                     "
     gene            complement(1157..3583)
                     /locus_tag="VGP50_00010"
     CDS             complement(1157..3583)
                     /locus_tag="VGP50_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEV8025773.1"
                     /translation="MDSSTLGARGRVLPVARHAFSLAGIAGAVAGAMLGGKVPALAAE
                     SDPASQQAQATPDAAPAPQPSGPQGAATPTFEEVTTTARQRAENVQDVPIPVTVLSGD
                     HLEDTGTDKFQQLQFAAPSTSVFLSNPRQAGIAIRGLGNNPANDGLQGSVGIYVDGVY
                     FDRDAMASFGLYDINQIEVLRGPQGTLFGKNTTAGALNITTNAPTFTPEVDTGITYGS
                     FNTQEYTAVINGPIVDNVLAGRLSIESDSHSGYVKSTEGNSYDGLNLKDARGQLLFQD
                     GDIFSLRLSGDYGLEHDNSGFSILYSPGPASSAKPGFTTFAEWSQRVGVPAFIDPNDF
                     KTNAVGEQVMKQETSGASATADWKLPDGFALTSISAIRTWSFQPQNNGFDYFAPPTIG
                     NGIPISQQDVSHDVQASEELRLASPVGGPVDFVAGLYYFWKRLAGDQETTFAADASTI
                     LGTNPVLNGAETKVDTDPTTNSAALFSQANWHISDRWTLTGGVRETFEQDTIHILQYP
                     VIDPASTPPASLGFFTASDTIRNWSLSGLISPSYKITQDVLAYLSLSHGAKAGGFNSP
                     AVPAFSGATFLPVSTLVVYPEKADDVELGVKSTLFDHRLTLNADAFLTRVTDYQANTT
                     IPLANGGNVSELLNVGAVTSKGFEVDAVARPIVGLSIVASGSYDLAQYQSFRNAPAVE
                     GATAASQDLSGRPVVGAPKWTASASGTYTWPVAAETAAYVTAEYAYKSAQYGFIDDSS
                     FSRLNAYGIANFRVGTNIADRYDVALWVQNAFNTANFYSTSTVTSGLGGYTAAPGIPR
                     VFGITVRAKF"
     gene            complement(3996..5252)
                     /locus_tag="VGP50_00015"
     CDS             complement(3996..5252)
                     /locus_tag="VGP50_00015"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8025774.1"
                     /translation="MIPTRFRHARALAIALTAGLLAGGATLGARAEESGGKNTVRPEI
                     GKPLQDAQKLIADRKYKEALAKLQQADGVRDKTAYESFVVEEIRGAAARGAGDMPTAI
                     RSFEAVVAANRLPPPDQLRYVQSLAVTYYTVKDYPQAIAWSSRYLKQGGTDGQVHTLL
                     AQSYYLAGDYTGATTELKAQLAAADRAGQSLPESALTMLADSALKQKDDAGYSEALQR
                     LVAAYPKTEYWLPLLHQIERAPGFAGRLQLDLDRIKLATGTLRGSGDYLAMAQLALAG
                     GLPGEARKIVEQGYAAGILGSGADAERHRRLRDLAEKSAAEDRNTLDKGIGEAAAQKD
                     GGGLVNTGLDYLGYGETEKGVGLIERGIEKGNLRRPEDGKLHLGLAYIAAGQKAKAIE
                     ILKTVQGSDGTADIARAWILFARAST"
     gene            complement(5283..5705)
                     /locus_tag="VGP50_00020"
     CDS             complement(5283..5705)
                     /locus_tag="VGP50_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEV8025775.1"
                     /translation="MAMTVGGASDDAEVMMEINTTPLIDVMLVLLIMLIITIPIQTHA
                     VKLDMPVTNPPAPPTPPVVVDITVDFDGTVSWNGQPLADQRQLEGLLRQAAQQPDQPE
                     VHLRPNKLVTYRSVAAVLAAAQRLGVTKVGLVNEQLTD"
     gene            complement(5739..6167)
                     /locus_tag="VGP50_00025"
     CDS             complement(5739..6167)
                     /locus_tag="VGP50_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEV8025776.1"
                     /translation="MAITFETEGGDEETLVSTINTTPLVDVMLVLLIIFLITIPVAIH
                     TVAVKLPPEANQPTQTKPENVVLSVTKDGDVYWNDRLLPDLNVLLERLKQVSPQVPQP
                     EVHIRGDEAARYEFIGKVVVMCQRAGIAKVGFITLPPPRG"
     gene            complement(6170..7108)
                     /locus_tag="VGP50_00030"
     CDS             complement(6170..7108)
                     /locus_tag="VGP50_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEV8025777.1"
                     /translation="MIATPVRRNAAVLVFASCVLLAAALSRGACAAESAAPLSAPETT
                     APAAPSGAGAGAPADVAPAPVAAAPATEEVANPYGLAQLWSQGDPVARGTMVVLLIMS
                     IGTWYIAISKLIEQTRVLAQARAADATFWNTGSVEESIGRLKESNAFRQIAERGLSAT
                     EHHEGSLVERIDLHTWVTMSIQRSVDAITARLQRGLAFLATVGSTAPFIGLFGTVWGI
                     YHALVAIGLAGQASIDKVAGPVGEALIMTAIGLFVAVPAVLAYNWLVRRNKAVSERVK
                     AFSSDLHSVLLSGAAAPRPARAQQPALRAAAVARTA"
     gene            complement(7159..7866)
                     /locus_tag="VGP50_00035"
     CDS             complement(7159..7866)
                     /locus_tag="VGP50_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV8025778.1"
                     /translation="MTITASAGFTGAHEESPRVVRWSGAPPSLGDDGSRLRQAGGAAF
                     VVLLHVGLIIALAAGLGHQAVELIQAPFQTKLIEEAKAPPPDAPPPPPLLAQLPPPYI
                     PPPEVQLRQVPVAAPSNAITAVVHEKPPEPAPVPVLKAEPVRVPPALDPAHPCREPDY
                     PPMAKRLGEEGTVVLKFLIGVDGAVLDSKIESSSGHPRLDEAARAELGRCRFTPGTVD
                     GKPEPSWAVNRHVWKFQ"
     gene            complement(7903..8388)
                     /locus_tag="VGP50_00040"
     CDS             complement(7903..8388)
                     /locus_tag="VGP50_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV8025779.1"
                     /translation="MRLLLTILSAAMIVAAPLAQAAENAPQAAPAADYKYKTPKLDRA
                     HVDALLAKPGNLLLIDVRRPDEITAIGGFPVYLSLQADSVEKSFAFIPKGRTIVTVSN
                     HAHRAGAVGDALAAHGFKVAGAIGVEDYAAEGGTVTKIAPPAKSAEAAQPSGESRPAS
                     R"
     gene            8667..9161
                     /locus_tag="VGP50_00045"
     CDS             8667..9161
                     /locus_tag="VGP50_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV8025780.1"
                     /translation="MSSRFAAARRFAPVAASIIGAAIAVAALAQTPGPSPGRQALESR
                     KAVFTLIEANFRPLGAILKGDAPFDAAAAQKRADRIVFLAGLLDENFPESSNLGEPDS
                     KAKAALWEQRADFEQKLKRFQDHAAALVEVSATEKGATEAFDGAVAAAAQDCKGCHDA
                     YKTK"
     gene            9139..9867
                     /locus_tag="VGP50_00050"
     CDS             9139..9867
                     /locus_tag="VGP50_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEV8025781.1"
                     /translation="MTPTRQSDTGRGGAAEDEPRVWDLPVRIFHWTLVAAFLGAFVTD
                     RMGVAYFRWHARCGYVVLVLVAFRLVWGVVGTRHARFRNFLRGPRAILRYALDVARGR
                     AESHAGHNPLGALMVVALLLALGVEAVSGLFGNDEIVDVGPLYGSVSEALSLALTSLH
                     RRLFYWIAAAVALHVAAVLAHRLLGGERLIRAMITGRKPQASVGPQDAIQGSRTWLAA
                     AVVAVIAAVLVWVVDVVPIVFANN"
     gene            9884..12229
                     /locus_tag="VGP50_00055"
     CDS             9884..12229
                     /locus_tag="VGP50_00055"
                     /inference="COORDINATES: protein motif:HMM:NF019223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1549 and DUF1553 domain-containing protein"
                     /protein_id="HEV8025782.1"
                     /translation="MKRSLTKTYVALSLCASTALGMAAVALAEDKPADSPLKPASAFA
                     QPLWSWQPVKAPEIPVVSDKSWVRQPIDAFVLAKLDAQGLTPSPDADRATFIRRATLD
                     AWGLIPTPEEVAAFVDDRSPDAYGKLVDRLLGSPHYGERQARRWLDLARYADSSGFQV
                     DEGRPNIWRYRDYVINAFNSDKPYDLFIKEQIAGDELEPGNEQALIATGFMANYPDNS
                     NSRDLVQRKYQITTDVVDTIGQGVLATTWNCARCHDHKFDKISMKDYYGIQAFFANTS
                     FDQKIPLTTKGEGERDFEAKQATYQEATKAIRDKMKALLEPVKAEADKYYRERYLPDS
                     RDSVFKPEAQWTPLDRWINFRYSQVTRQEALAGYFARGGESKDSPDYNEADWGKWLEY
                     RALQRELKSFDNIKPKGSDTLTGVTELGHPDAPTTYVFFGGNHERPLEEVQPAFPHEI
                     TEEKPVIQATATSSGRRLAFANWLASPTNPLTARVYVNRVWDQYFGRGIVATVSDFGR
                     AGDKPSNPELLDDLADSFVKDGWSIKKLHRQILLSSVYRQSSVPREEAAKIDPDNKLL
                     AVFPRQRLEAEEIRDSLLLASGKLVDKVGGPSVAAPLPAGLTPPQQNNVTETMDAAKD
                     EEDQYRRSLYIITKRSLPYPMLDTFDMANSQQIHSRRSVTTTPLQALALLNSDIVFKL
                     SQHLAARVLAEAGSDESAQFDRLYQILFARDADADEKRLLTAFLDSHVAVVAQKAADG
                     SLSLALPVDWKPGAGFDPIRAAAFVDLVDTVVNTNDFIYRF"
     gene            12263..13717
                     /locus_tag="VGP50_00060"
     CDS             12263..13717
                     /locus_tag="VGP50_00060"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEV8025783.1"
                     /translation="MSKLSRREWIARAGYGMGGLALTSLLPGGGILAIPAEAAAADLA
                     GDDPLAPKAPHFPPKVKSVIWLHMDGAPSTLDLFDYKPELARLRGQEVPQSFLQGVKT
                     STQGGVGKLFEANRTWKQHGESAAWFSDLVPNLATQADKITFIKSSVTIGATHDISVL
                     KLNTSDVNPGRPSLGSWITYALGSANRDLPPYVVLYTGKKEPTGGSVNWSSGFLPAVY
                     AGTAFRPNGQPILNLQSPDAISAAQQRDNLDLLKSLNEHEAAAFPQDSELQARIRSYE
                     LAYRMETAAPEAVDLGKESEATKALYGLDEDATREYGTNLLRARRLVERGVRFVHVVT
                     GLHDIGGEDLNWDAHRNLLKNHDINARAVDKPIAGLLADLKARGLLDQTLVVWVSEFG
                     RTPYGESGDGRDHNPWGYTQWLAGAGLKAGITHGSTDELGLKAAENPVDTYDIQATVL
                     RLAGLDFRKVTYFRNGRSERPTQVYGRVIEEILA"
     gene            13806..14222
                     /locus_tag="VGP50_00065"
     CDS             13806..14222
                     /locus_tag="VGP50_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025784.1"
                     /translation="MKRSLLLALALAIVFAGSASRAEHIAVDLHDYDLDLMRSLDQTF
                     KYFEPDLADGNVKAYADDVQLVHYAFIWLESYFEKKGSDADQAVKIAQRGLLLLDGID
                     KDVAAKNFDAAADKAHELGLTCRSCHDVYKPLQTRS"
     gene            14281..14775
                     /locus_tag="VGP50_00070"
     CDS             14281..14775
                     /locus_tag="VGP50_00070"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV8025785.1"
                     /translation="MSRLPTLLLAAGLTAAAATVPAAAQLLTEKSLSTASALAVAEAA
                     YESCRAQGYHVSVHVVGREGQVLVGLRGDGSSPHTFENSQRKAYTARTFRTTSGEFAQ
                     RVKDNPGPGATVLTGIAAIQGGVPIRVGDDVIGAVGVSGAPGGDKDEACSKAGIDKIA
                     DQLR"
     gene            14951..15949
                     /locus_tag="VGP50_00075"
     CDS             14951..15949
                     /locus_tag="VGP50_00075"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEV8025786.1"
                     /translation="MSEALIKPTSFIVDRLARAAPATRRNPGQVPRWRRWELPTWGVA
                     LAVYVGFFVLTWNFRHIPLWLAAPLGSLLLAWHGSLQHETIHGHPTRSRRINALLGAP
                     PLALWMPYAVYRESHLRHHRQRGHDLTLPGRDPESHYLPAGTVARMGWFRRAIVTATT
                     TLAGRLALGPALAITSFWTQELRRIAAGDRLRRRLWLRHAAAASAVMIWVVGVCGISP
                     PIYVGLIVYPSLSLTLLRSFVEHRAARDASARTTAVEAHSAWALLFLNNNLHIAHHAR
                     PSLPWYDLPGLWRQLRPAVMDDPELVIRDGYGAIFRRYLLRPANTVEHPQLSRWPQ"
     gene            16000..>16640
                     /locus_tag="VGP50_00080"
     CDS             16000..>16640
                     /locus_tag="VGP50_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEV8025787.1"
                     /translation="MYDLPGLQAATDALWRALAQRLVAAGVDGVPDELTRGVPPAVVW
                     HEPHLLLAQSCGYPLVTSLSQAVKVVATPRYRAVGCEGAFARSAVVVPIGHPATTLAD
                     LRGSRCVINELASNSGMNLLRAAIAPLAAGRRFFRTVRVSFSHAESLRMVAAGEADIA
                     AIDCVTLAHLQRGRSSDLAAAVRVLFWTEPSPGLPLVTSRDTDDATVAAIRAAL"
BASE COUNT         2699 a         5594 c         5522 g         2825 t
ORIGIN      
        1 gtctggtcga aagaccggtt ctccagcatc agctggacga catgcttgat tttgccaggc
       61 tgcttgccgg tctgttgatg ctcgttggcg gccttctgag cttgattctt tgtcacaacg
      121 tcgtccttcc ctgcgaagcg cttccccggc gagcgtctcc ggggcgagct tttccgcgaa
      181 ctctccgcag cgtgcgctgg cgttgctgcg tcgctcggcg gcacgatttc tggagctctc
      241 gacaagcgat ggtgaaccgc agccgatacg gcgacggatg aagcacgatc ctagggactc
      301 ggatggatcg taaagttacg aactcatgtc tgctttgtga cggtgccgcg caagcatgct
      361 aggcgccgac cgcctcgata taggtcatga caagatacaa gatggcttgc ttgcttcccc
      421 ggttgcggta ggcgtgaggc acgtcggcgg cgaacatgat ggcgtcatcg cttctcagga
      481 catgcgacac gcccgcgacg acgatctcca cctggccggt ggcgacgacg agattctcct
      541 tggtgccggg ggcatgggga gccgcgatct cctcggtgcc ggggccgagg gtgagcttgt
      601 agaattcgac gcgccgctcc gcatcgaagg gaaacaatgc ccgggacgtg aaaccgccat
      661 cgaccgagct gagaatcttg gcattggcgc ccgggagcag gaccgtgccg ctctggctct
      721 cgtcgctgat cagcgccgcg aagggcaccc tgagcgcacg cgccacgcgc cagagcaggc
      781 cgatggtggg cacgctgcga gccagttcga tctgacgcag catgccgcgg ctgacgccgg
      841 cctccttgga cagttgctcg agcgacagct cgcgcccgac gcgcagccgc ctcagattct
      901 cgctcacggt ccgggcaacc gccgcgactt ccgcttcctt gctcggcgtg gccgctgacg
      961 agactttggc cgcccgcttg ggcgggccag cctcgcgacg cttcttggcc ctcactccca
     1021 gacaactccg ccttgacgcc gcgcgcccgc cggcggcgac gcgcgccctt tacgggtcca
     1081 tggctgttct cttgccgggc cgatcttatc ggcgatgccc ggagcgtcaa ctcgggccgt
     1141 gacccgacgt tgcgcgtcag aactttgccc gcaccgtgat gccgaagacg cggggaatgc
     1201 ccggagcggc cgtgtagccg ccgaggccgc tggtcacggt cgaggtggag tagaaattcg
     1261 cggtgttgaa agcgttttga acccagagcg ccacgtcgta gcggtcggca atgttggtgc
     1321 cgacgcggaa attcgcgatg ccataggcgt tcagacggct gaagctcgaa tcgtcgatga
     1381 agccgtactg cgcggacttg taggcatatt cggcggtgac gtaggccgcc gtctcggcgg
     1441 cgaccggcca tgtgtaggtg ccgctcgcgc tcgccgtcca tttgggcgcg ccgaccacgg
     1501 ggcggccgct cagatcctgg ctggcggccg tcgcgccctc gacggccgga gcattgcgga
     1561 aggattggta ttgcgcgagg tcataggacc ccgaggcgac gatgctcagg cccacgatcg
     1621 gccgggcgac ggcatcgacc tcgaagccct tgctggtgac ggcaccgaca ttgaggagct
     1681 ccgaaacatt accgccattg gcgagcggga tcgtcgtatt cgcctgatag tcggtgacgc
     1741 gtgtcaagaa cgcgtcggcg ttgagggtca ggcgatggtc gaagagggtg ctcttgacgc
     1801 cgagctcgac gtcgtcggcc ttttcgggat agacgacgag ggttgacacc gggagaaacg
     1861 tcgcgccgga gaaggccggg accgccggcg agttgaagcc gcccgccttg gcgccatgcg
     1921 acagcgacag ataggcgagc acgtcttgag tgatcttgta gctcgggctg atcaggccgc
     1981 tgagactcca attccggatc gtgtcgctcg ccgtgaagaa gccgagagag gcgggcggtg
     2041 tgctcgccgg gtcgatcacc ggatactgga gaatgtggat cgtgtcctgc tcgaaggtct
     2101 cgcgcacgcc gccggtcaag gtccagcggt cgctgatgtg ccagttcgcc tggctgaaga
     2161 gcgcggcgct gttggtcgtc gggtcggtgt cgaccttggt ctccgcgccg ttcaggaccg
     2221 ggttggttcc gaggatcgtc gaggcgtccg ccgcgaaggt ggtctcttga tcgccggcga
     2281 ggcgcttcca gaaataatag aggccggcga cgaaatcgac cggtccgccc accggcgacg
     2341 cgaggcggag ctcctcgctc gcctgaacgt cgtggctcac gtcctgctgc gatatgggaa
     2401 tgccgttgcc gatcgtcggc ggcgcaaaat agtcgaagcc gttgttctgc ggctggaagc
     2461 tccaggtgcg gatcgccgag atcgaggtca gcgcaaagcc gtcgggcagc ttccaatcgg
     2521 ccgtggctga agcgccggaa gtttcctgct tcatcacctg ctcgccgacg gcgttggtct
     2581 tgaagtcgtt ggggtcgatg aacgccggca cgccgacgcg ctgggaccat tcggcgaagg
     2641 tggtgaagcc gggctttgcc gagctggccg gcccggggct gtaaaggatg ctgaagccgc
     2701 tgttgtcgtg ctcgagcccg tagtcgccgc tcaggcgcag cgaaaagata tcgccgtcct
     2761 ggaaaaggag ctggccccgg gcgtccttaa ggttgagccc gtcataggaa ttgccttcgg
     2821 tcgacttcac gtagcccgaa tggctgtcgc tctcgatgga gagccgcccc gccaggacat
     2881 tgtcgacgat cgggccgttg atcacggccg tgtattcctg ggtattgaag gacccgtagg
     2941 tgatgccggt atcgacttcg ggcgtgaagg tcggcgcgtt ggtggtgatg ttgagcgccc
     3001 cggcggtggt gttcttgccg aacagcgtgc cttgaggacc gcgcagcact tcgatctggt
     3061 tgatgtcgta gaggccgaag ctcgccatcg catcgcgatc gaagtagacg ccgtcgacgt
     3121 agatcccgac gctcccctgc aacccgtcat tggccggatt gttgccgaga ccgcgaatgg
     3181 cgataccggc ctggcgggga ttggacagga aaacgctggt cgagggcgcg gcgaattgca
     3241 gctgctggaa tttatccgtg ccggtatcct cgagatggtc gccgctcagc accgtcacgg
     3301 gaatcggcac gtcctgcaca ttctccgcgc gctggcgcgc cgtggtcgtc acttcctcga
     3361 aggtcggggt cgctgcgcct tgcggcccgc tcggctgcgg tgccggcgct gcgtcgggcg
     3421 ttgcctgggc ctgctgagaa gcgggatcgg actctgcggc caaggccggc accttgccgc
     3481 cgagcatcgc gccggcgacc gcgccggcga tgccggcaag agaaaaagcg tggcgagcga
     3541 ccggcaggac tctgccgcgc gcccccaaag tgctgctgtc cattcgtgat acccccacat
     3601 cggctgcgtg ccgttttctg gccgctcgat gcgagtggcc actgcgcgcg cagaaactgg
     3661 cacggctcga tcctcgccgt caaaatagta tctctttagt caatatattg ctgaggttta
     3721 ccttcgtcgc cgcggcttcc cagctgaaaa ttttcctaga ccacgttcca ctgaacttgc
     3781 agcgcgcgac caagaacgct gcaatacggc catgcgtcct tcgacaggct caggatgagg
     3841 aagaacgaga atgacatctg agtggggccg caaagaagaa tcctcatcct gagcttgtcg
     3901 aaggacgcac gggctggact gcagcgtgcg tccacgtgtt gcgcctgtgc gcgagcaaac
     3961 aaaatgcagg tcgctgactg tgcccggatc cgccctcagg tcgaggcgcg ggcgaagagg
     4021 atccaggcgc gggcgatgtc cgcggtgccg tcgctgccct gcacggtctt gaggatctcg
     4081 atggccttgg ccttctgccc tgcggcgata taagcgaggc cgagatgcag cttgccatcc
     4141 tccggacgcc tgaggttgcc tttctcgatg ccgcgctcga tgagcccgac gcccttctcg
     4201 gtctcgccat atcccagata gtcgagaccg gtattgacga ggccgccgcc atccttctgt
     4261 gcggcggctt cgccgatgcc cttgtcgagc gtgttgcgat cctcggccgc ggatttctcg
     4321 gcaaggtcgc gcaggcgccg gtgccgctcc gcatcggcgc cgctgcccag gatccccgcg
     4381 gcatagccct gctcgacgat tttccgcgcc tcgcccggga gtccgcccgc cagcgcgagc
     4441 tgggccatgg cgagataatc gccggagccg cgcagcgtgc cggtggcgag cttgatgcgg
     4501 tcgagatcga gctgcaggcg cccggcaaat cccggcgcgc gctcgatctg gtggagcagc
     4561 ggcagccagt actcggtctt gggataggcg gccacgagac gctgcagcgc ctcggaatag
     4621 ccggcatcgt ccttctgctt gagcgcgctg tcggcgagca tggtgagcgc gctctccggc
     4681 aggctctggc cggcgcggtc ggcggcggcg agctgcgcct tgagctcggt ggttgcgccg
     4741 gtatagtccc cggcgaggta ataggattga gcaagcagcg tatgcacctg cccgtcggtg
     4801 ccgccctgct tcaagtaccg gctcgaccac gcgatggctt gcggatagtc cttgaccgtg
     4861 tagtaggtca ctgcgagcga ctgcacgtaa cgcagctggt cgggcggcgg cagccggttc
     4921 gcggcgacca cggcctcgaa ggagcggatc gcggtcggca tgtcgccggc gccgcgcgcc
     4981 gccgcgccgc ggatctcttc gacgacgaag ctctcatagg ccgtcttgtc gcggacgccg
     5041 tcggcctgct gcagcttcgc cagcgcctcc ttgtatttgc gatccgcgat gagtttctgc
     5101 gcgtcctgaa gcggcttgcc gatttcgggg cggacggtgt tcttgccgcc gctctcttcc
     5161 gcccgtgcgc cgagcgtggc gccgccggcc agaagccccg ccgtcagcgc gatcgcgagc
     5221 gcgcgcgcat gccgaaaacg cgttgggatc atggccgtga ctcctctcga aaacatcagc
     5281 tctcagtccg tgagttgctc gttgaccagc cctaccttgg tcacgcccag ccgctgcgcc
     5341 gcggcgagga cggcggcgac gctgcgatag gtgacgagct tgttgggacg cagatgcact
     5401 tcgggctgat cgggctgctg cgccgcctgc cgcagaagcc cttcgagctg gcgctggtcg
     5461 gcgagcggct ggccgttcca cgacaccgtc ccgtcgaaat cgacggtgat gtcgacgacc
     5521 accggcggcg tcggcggcgc cgggggattg gtcacgggca tatcgagctt caccgcatga
     5581 gtctggatcg ggatggtgat gatcagcatg atcaggagca cgagcatgac gtcgatcaac
     5641 ggcgtcgtat tgatctccat catcacctcc gcgtcgtcgc tcgcgccgcc cactgtcatg
     5701 gccatggcct gtctcctctc gtccccgtcg tccccggctc agccgcgcgg cggcggcaag
     5761 gtgatgaatc ccaccttggc gatgcccgcg cgctggcaca tgacgaccac tttgccgatg
     5821 aattcgtagc gggcggcctc gtcgccgcgg atatgcacct cgggctgcgg aacctgcggc
     5881 gacacctgct tcagccgctc gagcagcacg ttcaaatcgg gaagcagccg gtcgttccaa
     5941 tagacgtcgc cgtccttcgt caccgacagc acgacgttct cgggcttggt ctgcgtcggc
     6001 tgattcgcct cgggcggcag cttgaccgcg accgtgtgga tggcgacggg aatggtgatg
     6061 aggaagatga tgagcagcac cagcatgacg tcgaccagcg gcgtcgtgtt gatggtggag
     6121 acgagcgtct cctcgtctcc accctcggtc tcgaaggtga tcgccatggt caagccgtcc
     6181 gcgccaccgc cgcggcgcgc agcgccggct gctgcgcccg cgcgggccgc ggcgcggcgg
     6241 cgccgctcag cagcaccgaa tggagatcgg agctgaaggc cttcacccgc tcgctcaccg
     6301 ccttgttgcg gcggaccagc cagttgtagg cgagcaccgc cggcaccgcg acgaagaggc
     6361 cgatcgccgt catgatgagc gcctcgccga caggccccgc caccttgtcg atggacgcct
     6421 gcccggcgag gccgatcgcg acgagggcat ggtagatgcc ccacaccgtg ccgaagagac
     6481 cgatgaaggg cgcggtcgag ccgaccgtcg cgagaaaggc aaggccgcgc tgcagccgcg
     6541 cagtgatggc gtcgaccgag cgctggatcg acatggtcac ccaggtgtgg aggtcgatgc
     6601 gctcgacgag agagccttca tgatgctccg tggcgctgag cccgcgctcg gcgatctggc
     6661 ggaaggcatt gctctccttg agacggccga tgctctcctc gacggacccg gtgttccaga
     6721 aggtcgcatc ggcggcccgc gcctgcgcca gcacgcgcgt ctgctcgatg agcttagaga
     6781 tcgcgatgta ccaggtgccg atcgacatga tgagcagaac gaccatggtg ccccgcgcaa
     6841 cgggatcgcc ctggctccag agctgagcaa gaccataggg attggcaacc tcttcggtgg
     6901 cgggcgcggc cgcgaccggc gccggcgcga cgtcggccgg tgcgccggca ccggcgcccg
     6961 atggcgccgc cggcgccgtg gtctccggtg cggagagcgg cgcggcactc tccgccgcgc
     7021 acgcgccgcg cgagagcgcg gccgcgagca ggacgcagct tgcgaaaacc agaaccgcag
     7081 cgttgcggcg aacaggggta gcgatcatga cgcctccttt tcggcggctg ccgcggcgcg
     7141 ccgcgccgca gcctccgctc actgaaactt ccagacgtga cggttgacgg cccaggacgg
     7201 ctcgggcttg ccgtcgacgg tcccgggcgt gaaccggcag cggccgagct cggcgcgcgc
     7261 ggcctcgtcg agccgcggat gcccgctcga tgattcgatc ttgctgtcga gaacggcgcc
     7321 gtcgacgccg atcaggaatt tgagcaccac cgtgccttcc tcgcccaggc gcttcgccat
     7381 gggcggatag tcgggctccc ggcagggatg cgcgggatcg agcgccggcg gcacgcgaac
     7441 gggctcggcc ttgagcaccg gcacgggcgc gggctcgggc ggcttctcat gcacgacggc
     7501 ggtgatggca ttgctcggcg cggcgacggg gacctgacgg agctgcacct cgggcggcgg
     7561 gatgtagggc ggcggcaatt gcgcgagcag gggcgggggc ggcggtgcgt ccggcggcgg
     7621 cgccttcgcc tcctcgatga gcttggtctg gaagggcgcc tggatcagct ccaccgcctg
     7681 atgaccgaga ccggcggcaa gcgcgatgat caagccgaca tggaggagga cgacgaacgc
     7741 ggcgccgccc gcctggcgca gacggctgcc gtcatcgccg agcgacggtg gtgcgccgct
     7801 ccatcgcacg acgcgcggcg attcctcgtg agcgccggtg aacccggcag aagccgtgat
     7861 cgtcatattc gttggaccct gtgcagcgac aagaacggtc aatcaccgcg aagccgggcg
     7921 cgattcgccc gagggctgcg ccgcttccgc cgacttggcc ggcggggcga tcttcgtgac
     7981 ggtgccgccc tcggctgcgt agtcctctac gccgatcgcg ccggcgacct tgaatccatg
     8041 agcggccagc gcatcgccga cggcgcccgc gcgatgcgcg tggttggaga cggtgacgat
     8101 ggtccggccc ttgggaatga aggcgaagct tttctcgacg ctgtcggctt gcaggctcag
     8161 atacaccgga aagccgccga tcgcggtgat ctcgtcgggc cggcggacat cgatgagcag
     8221 cagattgccg ggcttcgcca gcagcgcgtc gacatgcgcg cgatcgagct tcggcgtctt
     8281 gtatttgtag tcggccgccg gcgccgcttg cggcgcattc tccgcagcct gcgcgagcgg
     8341 cgctgcaacg atcatcgctg cgctcaagat cgtaagaaga agcctcatgg cgatcgcctt
     8401 ccctgctgaa gctgcccgtg ccggcggtgc ctcgccggcg tcgagcgcgg gacgccatgt
     8461 cgccatatgt tatcgcatac ggtcaatatc ttatcgcgcg cgccatcgat agttcttgtg
     8521 tgcgtcgtcg atataatatc ttgcccgtca atatattgac gggcgccggc gctctctggc
     8581 acgcttgccg tcgactgaac gccggccggc atcgtctttc gcaccggcgc agcgatcgcg
     8641 gtgggatgcc gacgagagat gacgtgatga gcagcagatt cgccgcggcc cgccgcttcg
     8701 ctccggtcgc ggcgtcgatc atcggcgccg ccatcgccgt cgccgcgttg gcgcagacgc
     8761 ccgggccgtc gccgggccgc caggcgctcg aatcacgcaa agccgtcttc acgctgatcg
     8821 aggcgaattt ccgtccgctc ggcgcgatcc tgaaaggcga tgccccgttc gacgccgcgg
     8881 cggcgcagaa gcgcgccgat cgcatcgtct tcctcgccgg acttctcgac gagaacttcc
     8941 cggagtcgtc gaatcttggc gagcccgata gcaaggcgaa ggccgcgctc tgggagcagc
     9001 gcgccgattt cgagcagaag ctgaagcgct tccaggacca cgccgctgcg ctggtcgagg
     9061 tgagcgcgac cgagaaaggc gcgacggagg cgttcgacgg cgccgtcgcc gctgccgcac
     9121 aggactgcaa gggctgccat gacgcctaca agacaaagtg acacgggccg cggcggcgcc
     9181 gcggaagacg agccccgagt ctgggacctg ccggtgcgga tttttcattg gacgctggtc
     9241 gcggcgttcc tcggcgcctt cgtcacggac cgcatgggcg tcgcctattt ccgatggcat
     9301 gcccgctgcg gctacgtcgt gctcgtgctg gtggcgttcc gcctcgtctg gggcgtggtc
     9361 ggcacgcggc acgcgcggtt ccggaatttc ctccgcggcc cgcgtgcgat cctgcgctac
     9421 gcgctcgacg tcgcgcgcgg ccgcgcggag agccatgccg ggcacaatcc gctgggcgcg
     9481 ctgatggtgg tggcgctgct gctggcgctc ggcgtcgaag ccgtcagcgg cctcttcggc
     9541 aatgacgaga tcgtcgatgt cggcccgctc tacggctcgg tgagcgaggc gctcagcctc
     9601 gcgctcacct cgcttcaccg ccgcctcttc tactggatcg ccgccgccgt cgccctgcat
     9661 gtggccgccg tgctggcgca ccgcctcctt gggggcgagc ggctgatccg cgccatgatc
     9721 accggccgca agcctcaggc gagcgtcggg ccccaagacg cgatccaagg ctcgcgcacc
     9781 tggctcgccg cagcggtggt cgcggtcatc gccgcggttc tggtttgggt cgtcgacgtc
     9841 gtgccgatcg tattcgccaa caattgacgg ggaagagagc accatgaagc gaagcctgac
     9901 gaagacatac gtcgcgttgt ccttgtgcgc ctcgacggcg ctcggcatgg cggcggtcgc
     9961 gctcgccgaa gacaagcccg ccgacagccc gctgaagccg gcaagcgcct tcgcgcagcc
    10021 gctttggtcc tggcagccgg tgaaggcgcc ggagatcccg gtggtctcgg acaaatcctg
    10081 ggtgcgtcag ccgatcgatg ccttcgtcct ggcgaagctt gacgcgcagg ggctgacacc
    10141 gtcgcccgac gccgaccggg ccaccttcat ccgccgcgcc acgctcgatg cctggggcct
    10201 gattccgacg ccggaagagg tcgcggcctt cgtcgacgac cgctcgcccg atgcctatgg
    10261 gaagctggtc gaccgcctcc tcggctcgcc gcactatggc gagcggcagg cgcggcgctg
    10321 gctcgacctc gcgcgctacg ccgacagctc gggcttccag gtcgatgaag ggcggcccaa
    10381 catctggcgc taccgcgact acgtgatcaa tgccttcaac agcgacaagc cctacgactt
    10441 gttcatcaag gagcagatcg ccggcgacga gctcgagccg ggcaacgagc aggcgctgat
    10501 cgccaccggc ttcatggcca actatccgga caacagcaac tcgcgcgacc tcgtccagcg
    10561 gaagtatcag atcaccacgg acgtcgtcga cacgatcggc cagggcgtgc tcgcgaccac
    10621 ctggaactgc gcccgctgcc acgaccacaa attcgataag atctcgatga aggactacta
    10681 cgggatccag gccttcttcg ccaataccag cttcgaccag aagatcccgc tgaccaccaa
    10741 gggcgagggc gagcgcgact tcgaggcgaa gcaggcgacg taccaggaag ccaccaaggc
    10801 gatccgcgac aagatgaagg ccctgctcga gccggtcaaa gcggaggccg acaaatacta
    10861 cagggagcgc tatctgccgg acagccgcga ctccgtcttc aagccggagg cgcagtggac
    10921 gccgctcgat cgctggatca acttccgcta cagccaggtc acgcgccagg aagcgctcgc
    10981 gggatacttc gcgcggggcg gcgagagcaa ggacagcccc gactacaatg aggcggactg
    11041 gggcaagtgg ctcgaatatc gggcgcttca gcgcgagctc aagagctttg acaacatcaa
    11101 gcccaagggc tccgacacgc tcacgggcgt gactgagctt gggcatcctg acgcgccgac
    11161 cacctatgtc ttcttcggcg gcaaccacga gcgtccgctc gaggaggtgc agccggcctt
    11221 cccgcacgag atcacggagg agaagccggt gatccaggcg accgcgacct catcgggccg
    11281 ccgcctcgcc tttgccaact ggcttgccag cccgaccaac ccgctcaccg cgcgggtcta
    11341 tgtcaatcgc gtctgggacc agtatttcgg ccggggcatc gtggcgacgg tcagcgattt
    11401 cggccgcgcc ggcgacaagc ccagcaatcc cgagctgctc gacgatctcg ccgacagctt
    11461 cgtcaaggac ggctggagca tcaagaagct ccaccgccag atcctgctct cgagcgtcta
    11521 ccgccagtca tcggtgccgc gcgaggaggc ggccaagatc gaccccgaca acaagcttct
    11581 ggcggtgttc ccgcgccagc gcctcgaggc cgaggagatc cgcgactcgc ttctcctcgc
    11641 ctcgggcaag ctcgtcgaca aggtgggcgg cccgagtgtc gccgcgccgc tgccggcggg
    11701 cctgacgccg ccgcagcaga acaacgtcac cgagacgatg gatgccgcca aggacgagga
    11761 ggaccagtac cggcgcagcc tctacatcat caccaagcgc agcctgccct atcccatgct
    11821 cgacaccttc gacatggcga attcgcagca gatccacagc aggcgcagcg tcaccacaac
    11881 gccgctgcag gcgctcgcgc tgctcaacag cgacatcgtg ttcaagctgt cgcagcatct
    11941 ggcagcgcgc gtgctcgccg aggccggcag tgacgagtcc gcgcaattcg accggctcta
    12001 ccagatcctc ttcgcgcggg atgccgatgc ggacgagaag cgccttctca cggcgttcct
    12061 cgacagtcac gtggcggtgg tcgcgcagaa agccgcggac ggcagcctct cgctggcgct
    12121 tcccgtcgac tggaagcccg gcgcgggttt cgatccgatc cgcgccgccg ccttcgtcga
    12181 cctggtcgat accgtcgtca acaccaacga cttcatctac cgcttctaac cgcttccctc
    12241 tcttcggacg aaaggaaaga tcatgtcgaa attgtcacgt cgcgaatgga tcgccagggc
    12301 cggatacggc atgggcggcc ttgcgctcac cagcctgctg cccggcggcg gcatcctcgc
    12361 cattcccgcc gaggccgctg cggcggatct cgccggcgac gacccgctcg cgcccaaggc
    12421 gccgcatttc ccgcccaagg taaagtcggt gatctggctg cacatggatg gcgcgccgag
    12481 cacgctcgac ctcttcgact acaagccgga gctcgccagg ctcaggggac aggaggtgcc
    12541 tcagtccttc cttcagggag taaaaacgag cacccagggc ggcgtcggca agctcttcga
    12601 ggcaaaccgg acctggaagc agcacggcga gagcgctgcc tggttctccg atcttgttcc
    12661 caatctcgcg acgcaggccg acaagatcac cttcatcaag tcgagcgtga cgatcggcgc
    12721 cacccacgac atctcggtcc tgaagctgaa caccagcgac gtcaatccgg gccggccgtc
    12781 gctcggctcc tggatcacct acgcgctggg ctccgccaat cgcgacttgc cgccctatgt
    12841 cgtgctctac accggaaaga aggagccgac gggcggctcg gtgaactgga gctcgggctt
    12901 cctcccggcc gtctatgccg gcacggcctt ccgccccaac ggccagccca tcctcaatct
    12961 ccagtcgccc gacgcgatct ccgcggcaca gcagcgggac aatctcgatc tgctcaagag
    13021 cctgaacgag cacgaggccg ctgcctttcc gcaggacagc gagttgcagg cgcgcatccg
    13081 ctcctacgag ctcgcctatc gcatggagac ggcggcgccg gaagcggtcg atctcggcaa
    13141 ggagagcgaa gccaccaagg cgctttacgg attggacgag gacgcgaccc gcgaatacgg
    13201 gaccaacctc ctgcgggcgc ggcgactggt cgagcgcggc gtgcgtttcg tccatgtggt
    13261 gacgggcctc cacgacatcg gcggcgagga cttgaactgg gacgcgcatc gcaacctgct
    13321 caagaatcac gacatcaatg cgcgcgcggt ggacaagccg attgccggac tgctcgccga
    13381 tctgaaggcg cgcggattgc tcgatcagac gctggtggtc tgggtgtcgg agttcggtcg
    13441 tacgccctat ggcgagagcg gcgacggacg cgaccacaac ccctggggct acacgcagtg
    13501 gctcgccggc gccgggctca aggcgggcat cacccatggc tcgaccgacg agctcgggct
    13561 caaggccgcg gagaatcccg tcgacactta tgacatccag gccacggtgc tgcgtctggc
    13621 gggcctcgac ttccgcaagg tgacctattt ccggaacggc cgctccgagc ggccgaccca
    13681 ggtctatggt cgcgtgatcg aggaaatcct cgcctagaac gacagcggtc ggcgcagccg
    13741 ccggcgacgg cggctgcgct tcgctgcgcg caccatccgc aaaagcctcg gggaaatttg
    13801 ttcaaatgaa gcgcagcctg ctccttgccc tggcgctcgc catcgtattc gccggcagcg
    13861 catctcgggc cgagcacatc gccgtcgatc tccacgacta cgatctcgac ctcatgcggt
    13921 cgctcgacca gaccttcaaa tatttcgagc cggacctcgc cgacggcaac gtgaaggcct
    13981 atgctgacga cgtgcagctc gtccattacg ccttcatctg gctcgagagc tatttcgaga
    14041 agaagggaag cgatgccgat caggcggtca agatcgcgca acgcggattg ctcctgctcg
    14101 acggcatcga caaggacgtc gccgccaaga acttcgacgc cgccgccgac aaggctcatg
    14161 agctcggctt gacctgccgt agctgccacg acgtctacaa gccgctgcag acgagatcct
    14221 gagcgggcgc cgaccccgcg cgtcgccccc gccattcccg cacagccctg gagaaaaccc
    14281 atgtcccgct tgccgaccct gcttctcgct gccggcctca ccgccgccgc ggcaaccgtg
    14341 cccgccgccg cgcagctcct caccgagaag agcctgtcga ccgcgagcgc gctcgccgtc
    14401 gctgaagccg cctatgaaag ctgccgcgcc cagggctacc atgtgtcggt ccacgtcgtc
    14461 gggcgcgagg gccaggtgct cgtcgggctg cgcggcgacg gatcctcgcc gcacaccttc
    14521 gagaacagtc agcgcaaggc ctataccgcg cggaccttcc gcaccacgtc gggcgaattc
    14581 gcccagcgcg tgaaggacaa tccggggccg ggcgccaccg tgctcacggg catcgccgcc
    14641 atccagggcg gcgtgcccat tcgcgtcggc gacgacgtca tcggcgccgt cggcgtgtcc
    14701 ggggcacccg gcggcgacaa ggatgaagcc tgctccaaag ccggcatcga caagatcgcc
    14761 gatcaactcc ggtgaccggg acggctatgc cgggttcatc acaaagtaga cccgctagaa
    14821 aaactttgcg taccaagaaa gattttctgc tttggcaaga agcgtccgcg cgggctaatg
    14881 ctcggcgtct atcgttgtcc cttcgctgtc gaacggcgcg cggcctgagg ctgagcgtca
    14941 gcgcaacacc atgtccgagg ccttgatcaa accgacatcg ttcatcgtcg atcggctggc
    15001 gcgtgccgcg ccggcgacac ggcgcaatcc cgggcaggtg ccgcgctggc ggcgctggga
    15061 gctgccgacc tggggcgtag cactcgcggt ctatgtcgga ttcttcgtgc tcacctggaa
    15121 tttccgccat atccccctct ggctcgccgc acccctgggc tctcttctgc tcgcctggca
    15181 cggatcgctg cagcatgaga cgattcacgg ccatccgacc cgttcgcgac gtatcaacgc
    15241 ccttctcggc gcgccgccgc tggcgctctg gatgccctat gcggtctatc gcgagagtca
    15301 tttgcgccat caccggcaac gcggacatga cctgacgctc cccggccggg atcccgaatc
    15361 ccactacctg ccggcgggca ccgtcgcgcg catgggttgg ttccgtcggg cgatcgtcac
    15421 ggcgaccacc acactcgctg gccgccttgc tcttggaccg gcgctcgcga tcacgtcttt
    15481 ctggacacaa gaactccgac ggattgccgc cggcgatcgg ctgcgacggc ggctctggct
    15541 ccgtcatgct gccgcggcat cggccgtcat gatctgggtc gtgggagtgt gcggcatatc
    15601 gccgccgatt tatgtcggct tgatcgtcta tcccagcctt tcgctcacgc tgctccgttc
    15661 tttcgtcgag catcgggcgg cgcgcgacgc gagcgcccgg acgacggccg tcgaggccca
    15721 ttctgcctgg gcgctgctgt tcttgaacaa caacctccac atcgcccacc atgcgcggcc
    15781 gtcgcttccc tggtacgact tgcccggcct ctggcggcaa ctccgtcctg ccgtcatgga
    15841 cgacccggag ttggtgattc gcgacggcta tggcgcgatc tttcgccgat acttgctccg
    15901 acccgccaac acggtcgagc atccgcagct ttcgcgatgg ccgcagtagc cggcaccgcg
    15961 cgcgccgtgg cgtccgctcg gcggatcgcg gcgctgccga tgtacgacct gccgggactc
    16021 caggccgcca ccgatgcgct gtggcgggcg cttgcacaac gcctcgtcgc cgccggcgtc
    16081 gacggtgtgc cagacgagct gacgcgcggc gttccgccgg cggtcgtctg gcacgagcct
    16141 cacctcctgc tggcccagag ctgcggctat ccgttggtca cgtcgcttag ccaggcggtc
    16201 aaggtcgtcg ccacgccccg ctaccgcgcc gtcggctgtg agggtgcgtt tgcgcgaagc
    16261 gccgtcgtgg tcccgatcgg tcaccccgcg acaacgctgg ccgatctgcg cggcagccgc
    16321 tgcgtcatca acgagctcgc atcgaacagc ggcatgaatc tgctccgcgc ggcgatcgcc
    16381 ccgctcgccg cggggcgtcg tttcttccgc actgtccggg tcagcttttc gcacgccgag
    16441 agcctgcgga tggtagcggc gggcgaggcg gacatcgcgg cgatcgattg tgtgaccctc
    16501 gcgcatttgc agcgagggcg gagctcggac ctcgccgccg ccgtgcgggt gctgttctgg
    16561 accgagccga gcccgggcct gcccttggtg acgtcgcgcg acaccgatga cgcgacggtc
    16621 gcggcaatcc gcgccgccct
//
LOCUS       DASYNS010000002         7714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3744873, whole genome shotgun sequence.
ACCESSION   DASYNS010000002 DASYNS010000000
VERSION     DASYNS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3744873"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..382)
                     /locus_tag="VGP50_00085"
     CDS             complement(143..382)
                     /locus_tag="VGP50_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025788.1"
                     /translation="MRVQVTFSVITIGLLLAACGSAPGDRALTGAGIGAAAGAVGGAL
                     IGVPALGAALGASAGAVVGAATTPSQVDLGKPVWR"
     gene            complement(724..2754)
                     /locus_tag="VGP50_00090"
     CDS             complement(724..2754)
                     /locus_tag="VGP50_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV8025789.1"
                     /translation="MTDRPAGPRCAALVGPYLSGKTTLLEALLFASGTTHRRGSAREG
                     NTVGDHSAEARARQMSTELNVAAAKYLGDPWTFLDCPGSVELAWEAQGALLAADVAVV
                     VAEPETERVLTMSPLFKFLDDHQIPHMVFINKMDTATARVSEVLAALQSVSQKPLVLR
                     QVPLPDAEGKATGYVDLVSERAYRYKPGQASDLVKLPDEALNEERVTRAGLVEKLADF
                     DDKLLEQLLEDLQPSKEEIYRHLTQDLRRDLIVPVFLGSALQDHGVRRLLKALRHETP
                     AATETAARLGLPAGTRDTVAQVIKTYHAPHSGKLSLARVWSGQVTDGMTLGGVRVAGA
                     LRLTGAQQEKLGTAAPGDVVALARMESVATGTVLSTGSAPKLPSPAIPKPVYGLAIAA
                     EKRADDVKLTGAVAKLVEEDPTLHLEQNAELQEMILWGQGDIHLQIAIDRLRNKYNLQ
                     VVPRKPHLPYKETIRRGTAQHARFKRQSGGHGQFADIQIEVKPLPRGGGHSFIDSVVG
                     GAIPRNYIPAVEEGVRDYLKHGPLGFPVVDVSVNLISGQYHAVDSSDQAFKTAARMAM
                     TEAMQKCEPVLLEPIHFVAISVPSAFTSRVQRLISGRRGQILGYDAKPGWDGWDVVTA
                     NLPQSELHDLIVELRSVTLGVGTYTDRFDHLQELTGKLAERAMAGRSEAAAQ"
     gene            3000..3293
                     /locus_tag="VGP50_00095"
     CDS             3000..3293
                     /locus_tag="VGP50_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025790.1"
                     /translation="MTLRRFLSAVPALLAMALLAGCYYPPPQAYAPYPPCAPVANPDG
                     TTSASPNCYGYAPYAAYPAPYYYPYPAYYSPYYGYPPVAVGVGFGFGFRGRFR"
     gene            complement(3416..4618)
                     /locus_tag="VGP50_00100"
     CDS             complement(3416..4618)
                     /locus_tag="VGP50_00100"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV8025791.1"
                     /translation="MPFLADRLSRIKPSPTLAMTKRALEMKAAGRDIISLSAGEPDFD
                     TPKNIQEAAIAAMRRGETRYTAVDGTPELKRAVAAKFKNENGLDYKPEQIIVSTGGKQ
                     VLFNALLATVNPGDEVVVPAPYWVSYPEIVSLAEGTPVEVPCPENNGFKLLPEDLEAA
                     ITPKTKWLILNSPSNPTGAAYSATELRALADVLLRHPQVWVLTDDMYEHLVYDGFEFA
                     TIAQVEPKLYPRTLTLNGVSKAYCMTGWRIGFAGGPVELIKAMATLQSQSTSNPSSIS
                     QAASVEALNGPKDFIPKHNASFKERRDLVVEMLNRAPGIRCPKPEGAFYVYPSCAGAL
                     GKRTPKGKAIETDTDFVEYLLGAEGVAVVQGVAFGLSPHFRISYAASTEALRDACARI
                     QRACAALK"
     gene            4843..5250
                     /locus_tag="VGP50_00105"
     CDS             4843..5250
                     /locus_tag="VGP50_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025792.1"
                     /translation="MNARFLVLAVPVLLFFVAGCAGTPMAIATDSDEQLRNEQTLDLC
                     RAYPMFLDQKGVRAELNRRLAVGDPEWVLIDQHKIYIGMSECALLASWGKPEKTDHDN
                     SLRSGGLLRYVYHGGADLVYVSDAKVTSFQKAN"
     gene            5348..7486
                     /gene="uvrB"
                     /locus_tag="VGP50_00110"
     CDS             5348..7486
                     /gene="uvrB"
                     /locus_tag="VGP50_00110"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEV8025793.1"
                     /translation="MLTTPRPSPPAGRPFAIRSEFEPAGDQPQAIVALGEGIERGERE
                     QVLLGVTGSGKTFTMAHVIQRTQRPALVLAPNKTLAAQLYGEMKSFFPENAVEYFVSY
                     YDYYQPEAYVPRSDTYIEKESSINEQIDRMRHSATRAILERRDTVIVASVSCIYGIGS
                     VETYSEMAFDLARGSRVNRTRLLAQLVELQYRRNDQNFMRGTFRVRGDSIEIFPAHYE
                     DRAWRLSLFGDELESIHEFDPLTGEKSGALDEITIYPNSHYVTPRPTLIQAIEQIKAD
                     LKFRLEEFHAQGKLLEAQRLEQRTTFDMEMMAATGSCAGIENYSRYLTGRKPGEPPPT
                     LFEYLPQDAMVFIDESHVTIPQLGGMFRGDYQRKSTLAEFGFRLPSCVDNRPLKFEEW
                     DAMRGQSVCVSATPGPWEMERAGGVFVEQVVRPTGLVDPVCLVRPVENQVDDLMAECR
                     DCALKGQRVLVTTLTKRMAEALTEYFHENGIRVRYLHSDVDTLERIEIIRDLRLGVFD
                     VLVGINLLREGLDIPECAVVAVLDADKEGFLRSRTALIQTVGRAARHIEGRAILYADR
                     MTDSMVAAIGETNRRREKQQAYNAAHGITPESVRRGISDILESVYEQDHFTVGLGESD
                     AVHYQGASLQTVLADLEKRMRAAAGDLEFEEAARLRDEIRRLEQLELGLPGAEDTGPR
                     PAGRSMAGRAGTSAKALAKAKARGRQRRRH"
BASE COUNT         1284 a         2616 c         2516 g         1298 t
ORIGIN      
        1 ggtggccgcc cctagatcca tcggctggat aacattgtcg gaccggggcg ccaagcgcgt
       61 attgcgcgca gtccggcggg actgcgcttc tccgcggcga ggatagtcca aaccctctcc
      121 cttcggggga gaaggatcgt ctttagcgcc agaccggctt gccgagatcg acctgcgagg
      181 gggtcgtcgc ggcgccgacg acagctccgg cgctcgcacc caaggcggca cccagcgcgg
      241 gcacgccgat cagcgcgccg ccgacagcgc ccgccgctgc gccgatcccg gcgccagtca
      301 gggcgcggtc gcccggcgca ctgccgcatg ccgcgagaag aaggccaatg gtgattaccg
      361 agaaagtcac ctgtacacgc attcttgtcc tccggagcag caaagttccc aagattctaa
      421 gtcgcgtcaa accatagcgc aaacagtcgt tggagatctg ctatcgccga tttatgtcat
      481 tttcggggcg agcctcgatt cgtctacgct ggatataaag cgctgccgcg gctgttacta
      541 ccggggacga aagaggtgcg acatgcgcgg aattctctta tgggctctcg gcatcccgat
      601 cccggtgatc atcctgctgt acgttttcca cgtcgtctga cggaccgggc tcgcctgggg
      661 ccagaaacgg cctcaggcga acagccgctc gctgttgagg ccgtcatagc agccggcgaa
      721 aggtcactgc gccgccgctt cgctgcgccc ggccatggcc cgctcggcga gcttgccggt
      781 cagttcctgg agatgatcga agcggtcggt gtaagtgccg acgcccagcg tcaccgagcg
      841 cagttcgacg atgaggtcgt gcagctcgga ttgcggcaaa ttcgcagtca ccacgtccca
      901 gccgtcccag cccggcttcg cgtcatagcc gaggatctgc ccgcgtcgtc ccgagatcag
      961 ccgctgcacg cgcgaggtga aggcgctcgg caccgagatc gcgacgaagt ggatgggctc
     1021 gagcagcacc ggctcgcatt tctgcatggc ctcggtcatc gccatgcgcg ccgccgtctt
     1081 gaacgcctgg tcggagctgt cgacggcgtg gtactggccg ctgatgaggt tgaccgacac
     1141 gtcgaccaca ggaaaaccca gcggcccgtg cttcagatag tcgcgcacgc cttcctcgac
     1201 ggccgggatg tagttccgcg ggatggcgcc gccgacgacc gaatcgatga agctgtggcc
     1261 gccgccgcgc ggcagcggct tcacctcgat ctggatatcg gcgaactgtc cgtggccgcc
     1321 cgactgccgc ttgaagcgcg catgctgcgc cgtgcccctg cggatcgtct ccttgtaggg
     1381 caaatgcggc tttcgcggca cgacctggag attgtatttg ttgcgcaagc ggtcgatggc
     1441 gatctgcagg tggatgtcgc cctgacccca caggatcatc tcctggagct cggcattctg
     1501 ctcgagatgg agcgtcgggt cctcctcgac gagcttggcg accgcgcccg tgagcttgac
     1561 gtcgtcggcg cgcttctcgg ccgcgatggc gaggccatag accggcttcg ggatcgccgg
     1621 cgagggcagc ttcggcgccg agcccgtcga cagcacggtg ccggtcgcga cgctctccat
     1681 gcgcgccagc gccaccacgt cgcccggcgc ggcggtgccg agcttctcct gttgcgcgcc
     1741 ggtgagccgc agcgcgccgg cgacccgcac gccgccgagc gtcatgccat cggtcacctg
     1801 gccgctccac acccgggcca gcgagagctt gcccgaatgc ggcgcgtgat aggtcttgat
     1861 gacctgcgcc accgtatcgc gcgtccccgc cggcaggccg agccgcgccg ccgtctcggt
     1921 ggcggccggc gtctcgtggc gcagcgcctt caggagacgc ctcacgccat ggtcctgaag
     1981 cgccgatccg aggaacaccg gcacgatcag atcgcgacgc aggtcctggg tcaaatggcg
     2041 gtagatctcc tccttcgagg gctgcaggtc ctcgaggagc tgctccagca gcttgtcatc
     2101 gaaatccgcg agcttctcga cgaggccggc gcgagtcacg cgctcttcgt tcagcgcttc
     2161 gtccggaagc ttgacgaggt ccgaggcttg tccgggcttg tagcgatagg cgcgctcgct
     2221 gacgaggtcg acatagcccg tcgccttgcc ctcggcatcg gggagcggga cctggcgcag
     2281 caccagcggc ttctgcgaca ccgattgcaa cgcggccagc acctcgctga cgcgcgccgt
     2341 ggcggtgtcc atcttgttga taaagaccat atgcgggatc tgatggtcgt cgagaaactt
     2401 gaagagcggg ctcatggtga ggacccgctc tgtttccggc tcggcgacga cgacggccac
     2461 atcggccgcc agtaaggcgc cttgcgcctc ccaggcgagc tccaccgagc ccgggcagtc
     2521 gaggaaagtc cagggatcgc ccaggtattt cgcggcggcg acgttgagct cggtcgacat
     2581 ctgccgcgcc cgcgcttcgg ccgaatggtc gccgacggtg ttgccttctc tggcgctgcc
     2641 gcggcggtgg gtggtgccac tggcgaagag cagggcctcg agcagagtcg ttttgccgga
     2701 gagatagggc ccgacaagcg cagcgcagcg cgggccagcg ggtctgtccg tcatgcacgc
     2761 ctccttcccc tgggtttcga gcgccgttca agcacgcgca agacggccac gcgggtgggc
     2821 gacgcctctt cggtcgaagg atggtcgcgc ggctgtcatc cgggtgcaat gggaatttcg
     2881 gccgcagccg cttacgcatt ctgcgttaga cgccggcgcg ctcttcgcag gcacgccggc
     2941 gccggatatc gcgtataatc ggggttggtt cggacgtttg ctcgcgtatg aggtggccca
     3001 tgacgcttcg ccgtttcttg tccgccgtcc cggctctcct tgccatggcc ctgttggccg
     3061 gctgctacta tccgccgccc caggcttacg cgccctaccc gccttgcgca cccgtcgcca
     3121 accccgacgg cacgacctcg gcgtcgccca actgctacgg ctacgcccct tacgccgcct
     3181 atcccgcccc ctattactac ccctaccccg cctactactc gccttactac ggctatccgc
     3241 ccgtcgcggt cggcgtcggt ttcggcttcg gcttccgcgg ccgcttccgc taggcgaacg
     3301 cgctacgcgc gttcgtccgc ggcggcgcca gcggtttgcg aagcaaactg cgagagccgc
     3361 accgagggga ctggcctgga ttgagagagg ggagaagcag cctccctcgt gaccgctact
     3421 tgagggccgc gcaggcgcgc tggatccgcg cgcaggcgtc gcgcagcgcc tcggtcgagg
     3481 cggcgtaaga gatgcggaaa tgcgggctca ggccgaaagc cacgccctgc actaccgcga
     3541 caccctcggc ccccagcaga tactcgacga aatccgtatc cgtctcgatc gccttgccct
     3601 tgggcgtgcg cttgccgagc gcgccggcgc aggagggata gacatagaac gcgccttcgg
     3661 gcttgggaca gcgaatgcct ggcgcccggt tcagcatctc caccaccaga tcccgccgct
     3721 ccttgaagga ggcgttgtgc ttggggataa agtccttggg gccgttgagc gcttcgaccg
     3781 aggccgcctg gctgatcgag ctcggattcg aggtcgattg cgactgcagc gtcgccatcg
     3841 ccttgatgag ctcgaccggc ccgccggcga agccgatgcg ccagccggtc atgcaatagg
     3901 ctttggagac gccgttcaag gtgagggtac gcggatagag cttgggctcg acctgcgcga
     3961 tggtggcgaa ttcgaagccg tcatagacga ggtgctcgta catatcgtca gtaagcaccc
     4021 agacctgggg atggcgcagc aggacatcgg ccagcgcgcg cagctccgtc gccgaatagg
     4081 cggcgccggt cggattcgag ggtgaattga gaatgagcca cttggtcttg ggcgtgatcg
     4141 ccgcctcgag atcctcaggc aacagcttga agccgttgtt ctcggggcaa ggcacttcca
     4201 cgggagtgcc ctcggcgagc gacacgatct cgggatagga gacccaatac ggcgccggga
     4261 cgacgacctc gtcgccggga ttgaccgtcg ccaggagcgc gttgaacagc acctgcttgc
     4321 cgccggtcga gacgatgatc tgctccggct tgtagtcgag cccgttctcg ttcttgaact
     4381 tggcggcgac cgcgcgcttg agctcgggcg tgccgtcgac cgcggtgtag cgcgtctcgc
     4441 cccggcgcat ggcggcgatc gccgcctcct ggatgttctt gggcgtgtcg aaatcgggct
     4501 cgccagccga gagagagatg atgtcgcggc ccgccgcctt catctcgagc gcgcgcttgg
     4561 tcatcgccag agtcggcgac ggcttgatcc gcgacaggcg atcggcgagg aagggcatgg
     4621 cgctaccgct acggctgttg gaagcgcgcc aaccctagtc ccgccggcgg agccgcgcaa
     4681 ccgcagagcc cgaaggtcag gcagcgaatt ttgccgcggt tcggccgcga gccggacgtc
     4741 gcccgcgatc gaaacgcaac aaaaaaacgc gatcacccgc ccacgaggtt atttccttcc
     4801 gacgagccat tcgactagat ttggcaaagg ggggaaagaa agatgaacgc acgctttctt
     4861 gttcttgctg tgcccgtttt gttgttcttc gtggcaggct gcgccggcac gccgatggcg
     4921 atcgcgacgg atagcgacga gcagcttcgt aacgagcaga cgctcgacct ttgtcgggcc
     4981 tacccgatgt ttcttgacca aaaaggcgtc cgcgccgagc tgaaccggcg tctggcggtg
     5041 ggcgaccctg aatgggtgct catcgaccag cacaaaatct acatcgggat gagcgaatgc
     5101 gccttgctgg cgtcctgggg caagcccgag aagaccgacc atgacaactc gcttcgaagc
     5161 ggtgggctgc ttcgctacgt ctatcacggc ggcgccgatc tcgtgtatgt cagcgacgcc
     5221 aaggtaactt cgttccagaa agccaactag acggtgcatc gccgcgaggg cacgggatgg
     5281 ttcgccttcc aggcccccgc tagcacagag gagccgcgcc ccttatctta gggtcatggc
     5341 gctaccgatg ctcacgaccc cccgcccgtc cccgccggcc ggccggccct tcgcgattcg
     5401 ctccgagttc gagcccgccg gcgaccagcc gcaggcgatc gtcgcgctcg gcgagggaat
     5461 cgagagaggc gagcgcgagc aggtgctgct cggcgtcacc ggctcgggca agaccttcac
     5521 catggcgcat gtcatccaga ggacgcagcg ccccgcgctc gtcctcgcgc ccaacaagac
     5581 gctggcggcg cagctctatg gcgagatgaa gagcttcttc cccgagaacg cggtcgagta
     5641 tttcgtctcc tattacgact attaccagcc cgaagcctac gtcccgcgca gcgacaccta
     5701 tatcgagaag gaatcgtcga tcaacgagca gatcgaccgc atgcgccact cggcgacgcg
     5761 cgcgatcctc gagcgccgcg acacggtgat cgtcgcctcg gtctcctgca tctacggcat
     5821 cggctcggtc gagacctatt cggagatggc cttcgacctc gcgcgcggca gccgcgtcaa
     5881 ccgcacccgg ctcctggcgc agctcgtcga gctgcaatac cgtcgcaacg accagaactt
     5941 catgcgcggc accttccgcg tccgcggcga ttcgatcgag atcttcccgg cgcattacga
     6001 ggaccgcgcc tggcggttgt cgctcttcgg cgacgagctc gaatcgatcc acgaattcga
     6061 tccgctcacc ggcgagaaga gcggcgcgct cgacgagatc accatctatc ccaacagcca
     6121 ctacgtcacg ccgaggccga cgctgataca ggcgatcgag cagatcaagg ccgacctcaa
     6181 attccgcctc gaggagttcc atgcgcaagg caagctgctc gaggcgcagc gccttgagca
     6241 gcgcaccacc ttcgacatgg agatgatggc ggcgacggga agctgcgccg gcatcgagaa
     6301 ctactcgcgc tatctcaccg ggcgcaagcc cggcgagccg ccgcccacct tgttcgagta
     6361 tctgccgcag gacgcgatgg tgttcatcga cgagagccac gtgaccatac cgcagctcgg
     6421 tggcatgttc cgcggcgact accagcgcaa gtcgacgctg gccgaattcg gctttcgcct
     6481 gccgtcctgc gtcgacaacc ggccgctcaa attcgaggaa tgggatgcca tgcgcggaca
     6541 gtcggtctgc gtctcggcga cgcccggccc gtgggagatg gaacgcgccg gcggcgtctt
     6601 cgtcgagcag gtggtgcgcc cgaccggcct ggtcgatccg gtctgcctcg tgcgcccggt
     6661 ggagaaccag gtcgacgacc tcatggccga atgccgcgac tgcgcgttga agggccagcg
     6721 cgtcctggtg acgaccctca ccaagcgcat ggccgaagcg ctcaccgagt atttccacga
     6781 gaatggcatc cgcgtgcgct acctccattc cgatgtcgac accctggagc gcatcgaaat
     6841 catccgcgac ctgcggctcg gtgtcttcga cgtgctggtc gggatcaacc tgctgcgcga
     6901 agggctcgac attcccgaat gcgccgtcgt cgccgtgctc gacgccgaca aggagggctt
     6961 cctgcgctcg cgcaccgcgc tcatccagac cgtcggccgc gccgcgcgcc acatcgaggg
     7021 gcgtgccatc ctctacgccg accgcatgac cgacagcatg gtggcggcga tcggcgagac
     7081 caaccggcgg cgcgagaagc agcaggccta caacgcagct catggcatca cgcccgagag
     7141 cgtgcgccgc ggcatctccg acattctcga gagcgtctac gagcaggatc acttcaccgt
     7201 cggcctcggc gagagcgacg ccgtgcatta tcagggcgcc agtcttcaga ccgtgctggc
     7261 cgatctcgag aagcgcatgc gtgccgccgc cggcgatctc gagttcgagg aggcggcgcg
     7321 gctccgcgac gagatccgcc ggctcgagca gctcgaactg gggctgccgg gcgccgagga
     7381 cacggggccg cgccccgccg gccgctccat ggccggccgc gccggcacct ccgccaaggc
     7441 cctcgccaaa gccaaagccc gcggccgcca gcggcgaaga cactgatggc gccgcgcgac
     7501 gcaaagcgtc gtcgcgggcg ggcccctctt ccctctcgga agagggaatt cgtgcggtgg
     7561 taatggagcc tcaaaccccg aaagccgcgc tcgtcaccgg cgccggccgg cgcatcggcc
     7621 gcgccatcgc gcttcgcctc gccgccgatg gctgggcggt cgccgtgcat tatcgctccg
     7681 cgcggaccga cgcggaaatg ctggcgggcg agat
//
LOCUS       DASYNS010000003         5008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_937234, whole genome shotgun sequence.
ACCESSION   DASYNS010000003 DASYNS010000000
VERSION     DASYNS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937234"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VGP50_00115"
     CDS             <1..674
                     /locus_tag="VGP50_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEV8025794.1"
                     /translation="QRRIGRLESAHRGTIFLDEIGDLPLELQAHLLRFLQERTIDRVG
                     GSTPIEVDARVICATNLDLAKAVREGRFREDLYYRLNVLSLRLPALRDRGDDIELLAT
                     FFLRKFAAEHGRTVAGFSDDARQRIREHSWPGNVRELIGRLRRAIVMAEGALIDAPDI
                     GLDATAPAARAVPAPARRGGPLLDEATLRQALQRHRHNVSNVARALDVSRMTVYRSLR
                     RYNIP"
     gene            complement(798..2324)
                     /locus_tag="VGP50_00120"
     CDS             complement(798..2324)
                     /locus_tag="VGP50_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEV8025795.1"
                     /translation="MPGSAAVPNRGLITVSIMLATIMQALDTTIANVALPYMQGSLSA
                     AQDQVTWILTSYIVAAAIMTPVTGWITGRFGLKRVFLVSVAGFTVASMLCGGATSLAE
                     MVVFRLLQGVFGAALVPLSQSVLLDINPREKQGQAMAIWGAGIMVGPILGPTLGGWLT
                     FNYDWRWVFYINLPVGILAFLGILVFVQDTKRDRGRRFDFLGFALLSLGVGALQIMLD
                     RGEQKDWFGSVEIIVECALAFIGFWMFAMWTGIADRPFLRLALFKDRNFLGSCILIFI
                     VGIILYATLALLPPMLQNLMDYPVTTAGLAMMPRGIGTMIAMLVVGRLITRIDVRLLL
                     LFGLGITTLSLYEMTHYSLTMDWWPIITVGVVQGVGLGFLFVPLSTIAFSTLDPARRA
                     EAAGFFSLLRNVGGSIGISMVETLQDRMTQTAHATLATQITPFAPALHSPLVQQVWNI
                     HTMAGLAAINQEITRQAAMIAYIDNFKFMMIVTLLAVPMLALLRKTRRAAPEMHAALD
                     "
     gene            complement(2336..3442)
                     /locus_tag="VGP50_00125"
     CDS             complement(2336..3442)
                     /locus_tag="VGP50_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV8025796.1"
                     /translation="MTTTTSERSWEGGRAMILRGLRSHGRLLRLVLMFGVPLAIVIAG
                     TAWYLSSGRYVATDDAYVQAGTVAVSTDVPGRVVAVEVLNNQYVKAGDVLFRLDDRPY
                     RAAVEQAEAQLANARLQVEAMRATYRQRLADLAGAQETLNYQQREYERQKQLLSSHVT
                     SQSSFDQVQNRWITARQQVTQVEQQMANALANLGGRADLPTEQHPMVQQMQAQLDQAK
                     LNLGYTVIRAPANGIVTNVDKLPVGNYLAASTPAFALVETDRPWVEANFKETELAHMK
                     PGDEATVTVDAYPDRTFKARVASLSPGTGSMFSVLPAQNATGNWVKVVQRLPVRLEVE
                     DMSPEQPLRAGMSADVSVDTQYRSPLARTLASLF"
     gene            complement(3439..3936)
                     /locus_tag="VGP50_00130"
     CDS             complement(3439..3936)
                     /locus_tag="VGP50_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV8025797.1"
                     /translation="MSDPTFDRSFGFLLHDIARLMGKRFEQRARSLGLSRAQWRTLAH
                     LQRCEGTNQSRLAELMGIEPISLARLIDRMEEAGLVERRSDPADRRAHRLYLTSRARP
                     NLEQCRVLGDAVRSEAFTGLADSDREALIDLLIRIRGNLSERRGDDERQRAALERGET
                     METAI"
     gene            complement(4158..>5008)
                     /locus_tag="VGP50_00135"
     CDS             complement(4158..>5008)
                     /locus_tag="VGP50_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8025798.1"
                     /translation="AEMPREAVWRGMTRDQLDAAYNNLDAVKNSAAKLAEWAARSEAV
                     RAARGELLDLPYGPRPRNRIDIFRCGTGGVPLLVFVHGGYWQRNNKEMFACIAEGPLA
                     RGLDVALIGYTLAPQVKLTEIVGEVCAALRWLRRAGPRFGIAARKLVLCGWSAGGHLT
                     AMALGEADAGLSISGIFDLEPCRLSYLNAALGLTVDEQIALSPIRHVSPGSGPLALAF
                     GTSELPELQRQSCAYGAAREAVGLPTEMLPLTGHDHFSILDELADPAGRLSEVVARLA
                     MAAAPA"
BASE COUNT          896 a         1643 c         1671 g          798 t
ORIGIN      
        1 tgcagcggcg catcggccgg ctcgaatcgg cgcatcgcgg caccatcttc ctcgacgaga
       61 tcggcgacct gccgctcgag ctgcaggcgc atctgctgcg cttcctccag gagcgcacca
      121 tcgaccgcgt cggcggcagc acgcccatcg aggtcgatgc ccgcgtcatc tgcgccacca
      181 atctcgatct cgccaaggcg gtgcgcgaag gccgcttccg ggaggatctc tactaccgcc
      241 tcaatgtgct gagcctgcgt ctccccgcgc tgcgcgaccg cggcgacgac atcgagctgc
      301 tcgccacctt cttcctgcgc aaattcgccg ccgagcatgg ccgcaccgtc gcgggcttca
      361 gcgacgacgc ccggcagcgg atccgcgaac actcctggcc cggcaatgtc cgcgagctca
      421 tcggccggct gcgccgcgcc atcgtcatgg cggagggcgc gctcatcgac gcgccggata
      481 tcggcctcga cgcgacagcg cccgccgcca gggccgtgcc ggcgccggcg cgccgcggcg
      541 gcccgcttct cgacgaggcc acgctgcgac aggcgctgca gcgccacaga cacaatgtga
      601 gcaacgtcgc gcgcgcgctc gacgtctcgc ggatgaccgt ctatcgctcg ctgcggcgct
      661 acaacatccc ttgacggcaa cggtggcttc gccggacccg atggcgaatt tcaacctgcc
      721 ccgctcggag atcgacgaca tcgtcgcgtg tctcgacggc ttgccgcgga ggcgaggatc
      781 gggcgggcag atctccacta gtcgagggcg gcgtgcatct cgggagcggc gcgccgggtc
      841 ttgcgcagca atgcgagcat cggcaccgcc agcagcgtca cgatcatcat gaacttgaag
      901 ttgtcgatat aagcgatcat cgccgcctgt cgcgtgatct cctgattgat cgccgccaga
      961 ccggccatcg tgtgtatgtt ccagacttgc tggacgagcg gcgaatgaag cgccggcgca
     1021 aacggcgtga tttgcgtcgc gagcgtcgca tgcgcggttt gcgtcatgcg atcctggaga
     1081 gtctcgacca tggagatgcc gatactgccg ccgacgttgc gcaggaggct gaagaagccg
     1141 gcagcttcgg cgcgccgcgc cggatcgagc gtggagaagg caatcgtgct caacggcacg
     1201 aagagaaacc ccaggccgac gccttgcacc actccgacgg tgatgatcgg ccaccagtcc
     1261 atggtcagcg aatagtgtgt catctcgtaa agcgagaggg tcgtgatgcc gaggccgaag
     1321 aggagcaaga gccgcacgtc gatgcgcgtg atcagccggc cgacgacgag catggcaatc
     1381 atcgtgccga taccgcgggg catcatggcg aggccggcgg tggtcaccgg atagtccatg
     1441 aggttctgca acatcggcgg caaaagtgcc agcgtcgcat agagaatgat gccgacgatg
     1501 aagatcagga tgcagctgcc gaggaaattg cgatccttga agagcgccag acgaaggaac
     1561 ggacggtcgg cgatgcctgt ccacatcgcg aacatccaga agccgatgaa cgccagcgcg
     1621 cattcgacga tgatctcgac ggagccgaac caatccttct gctcgccgcg gtcgagcatg
     1681 atctgcaagg cgccgacgcc gaggctgagc agcgcgaatc cgaggaagtc gaagcgtcgg
     1741 ccgcggtcgc gtttcgtgtc ctggacgaag acaagaatgc cgaggaacgc caggatgccg
     1801 accggcaggt tgatgtagaa gacccagcgc caatcgtagt tgaaggtgag ccaaccgccg
     1861 agcgtcggtc cgagaatggg tccgaccatg atgccggcgc cccagatcgc catcgcctgg
     1921 ccctgctttt cgcgcgggtt gatgtcgagc agcaccgatt gcgagagcgg gacgagagcc
     1981 gccccgaaaa caccctgaag caagcggaac acgaccatct cggcaaggct cgtcgcgccg
     2041 ccgcagagca tcgaggccac cgtgaagccg gcgaccgaga cgagaaagac ccgtttcaag
     2101 ccgaagcgtc cggtgatcca cccggtcacc ggcgtcatga tcgcggcggc gacgatatag
     2161 gaggtgagga tccaggtgac ctggtcctgg gccgccgaca agcttccttg catatagggg
     2221 agcgccacgt tggcgatcgt ggtgtcgagc gcctgcatga tcgtcgccag catgatggag
     2281 acggtgatga ggccgcggtt cggcacggcc gctgagccgg gcatcgatca gctcctcaga
     2341 agagagacgc cagcgtccgc gccagcgggc tgcgatactg cgtatcgacg ctgacgtcgg
     2401 cgctcatccc cgcgcgcagc ggctgctccg ggctcatgtc ctcgacctcg agccgcaccg
     2461 gcaggcgttg caccaccttc acccaattgc ccgtggcgtt ctgtgccggc aggacggaga
     2521 acatcgagcc ggtgccgggg ctcaagctcg cgacacgcgc cttgaaggtc ctgtcggggt
     2581 aggcgtcgac cgtgacggtc gcctcgtccc ccggcttcat atgcgcgagc tccgtctcct
     2641 tgaaattggc ctcgacccag ggacggtcgg tttcgaccag cgcgaaggcc ggggtcgatg
     2701 cggcgagata gttccccacc gggagcttgt cgacgttggt gacgatgccg ttcgccgggg
     2761 cgcggatgac cgtgtaacca agattgagct tggcttgatc gagttgcgcc tgcatctgct
     2821 gcaccatcgg atgctgctcg gtcggcaggt ccgcgcgacc gccgagattg gccagcgcgt
     2881 tggccatctg ctgctcgacc tgcgtcactt gctgccgcgc cgtgatccag cggttttgca
     2941 cctgatcgaa cgacgactgc gacgtcacat gagaggagag aagctgcttc tggcgctcgt
     3001 attcgcgctg ttgatagttg agcgtctcct gcgcgccggc gagatccgca aggcgctggc
     3061 ggtaggtggc gcgcatcgcc tcgacctgca gccgcgcatt ggccagctgc gcttcggcct
     3121 gctcgaccgc cgcgcgatag gggcggtcgt cgagtcggaa cagcacgtcg cccgccttga
     3181 cgtattgatt gttgagcacc tccacggcga ccacgcggcc ggggacgtcg gtgctgaccg
     3241 ccacagtgcc ggcctggaca taggcgtcgt cggtcgcgac atagcggccc gaagacaaat
     3301 accacgccgt ccccgcaatc acgatcgcca gcggcacgcc gaacatgagc acgagccgca
     3361 gcaagcgccc gtggcttctc agcccgcgca gaatcatcgc gcgcccgccc tcccagctcc
     3421 gctcggaagt cgtcgtggtc atatcgcggt ctccatcgtc tcgcctcgtt cgagagcggc
     3481 gcgttgcctc tcgtcgtcgc cacgtcgttc ggaaaggttg ccgcggatgc ggatgagcag
     3541 atcgatcagg gcctcgcggt ccgaatcggc caggccggtg aaggcctcgc tgcgcaccgc
     3601 atcgcccagc acacggcatt gctcgaggtt gggtcgagca cgtgaagtca ggtagagccg
     3661 atgcgctcgc cgatccgccg ggtcgctgcg gcgctccacg agccctgcct cttccatgcg
     3721 gtcgatgagg cgggcgaggc tgatcggctc gatccccatg agctccgcaa ggcgcgactg
     3781 gttggtgccc tcgcatcgct ggagatgcgc gagcgtccgc cattgggcgc gcgaaaggcc
     3841 gagcgagcga gcacgctgct cgaagcgctt gcccatcagc cgggcgatgt catgcagcag
     3901 gaagccgaag ctgcggtcga aagtcgggtc actcatgggc gagaatataa taagcgcgct
     3961 tatgttaagc ttggctttat tttcggaccc cagccatgca gcagacgcag gggtcaggcg
     4021 ctttcctgac atttgacgcg ttctgattga ataacaagaa cgtgaagaaa ccctgggtcg
     4081 accggccgcg gaagcaaacg gccgctcggc ggccagcgtc agggcgcgcg ctctcgccgc
     4141 tcggaaagcg ccgacggcta tgccggggca gcagccatgg ccaatcgcgc cacaacctcg
     4201 ctgagccgcc cggccgggtc ggcgagctcg tcgagtatcg agaaatgatc gtgaccggtc
     4261 agcggcagca tttcggtcgg caggccgacg gcctcccgtg ccgcgccata ggcgcatgac
     4321 tgacgctgca attcgggaag ctccgacgtt ccgaaggcga gcgccagcgg tcccgaaccc
     4381 ggcgagacat ggcggatcgg actgagcgcg atctgctcgt cgaccgtcaa gccgagtgcc
     4441 gcgttgaggt agctgagacg acacggctcg aggtcgaaaa ttccgctgat cgagaggccg
     4501 gcatcggcct cgccgagagc catcgctgtc agatgcccgc cggccgacca gccgcagaga
     4561 acgagcttgc gggcggcaat gccgaaacgc ggccccgccc ggcgcaacca gcgcaaagcg
     4621 gcgcacacct cgccgacgat ctcggtcagc ttgacctggg gtgccagcgt atagccgatg
     4681 agcgcgacgt cgaggccgcg cgcgagcggc ccctcggcga tgcaggcgaa catctccttg
     4741 ttgttgcgct gccagtaacc gccatgaacg aagacaagca gcggaacgcc gccggttccg
     4801 cagcggaaga tgtcgatgcg gttgcggggt cgcgggccat aaggcagatc cagcagttcg
     4861 ccgcgcgcgg cgcgcaccgc ctcgctgcgc gccgcccact cggcgagctt ggcagcgctg
     4921 ttcttgacgg cgtccaggtt gttgtaggcc gcgtcgagct ggtcgcgcgt catgccgcgc
     4981 cagacggcct cacgcggcat ttcggccg
//
LOCUS       DASYNS010000004         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2185413, whole genome shotgun sequence.
ACCESSION   DASYNS010000004 DASYNS010000000
VERSION     DASYNS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2185413"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..987)
                     /locus_tag="VGP50_00140"
     CDS             complement(211..987)
                     /locus_tag="VGP50_00140"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEV8025799.1"
                     /translation="MARRLIDVSMPLENEVASDPPGYGPRIEYLTHKNTAADVARFFP
                     GLKEEDLPDGEGWAIEWVRLMTHNGTHLDAPYHFHSTMNRGERAITIDEVPLDWCLQP
                     GVKLDFRHLPDGYVVTADDVARELDRIGHELRPLDIVVVNTRAGAAYGKPDYVAAGCG
                     MGRAATLHLLERGVRLTGTDAWSWDAPFVHTREKYTATQDARLIWEGHKAGREIGYCH
                     LEKLHNLEALPADGFTVSCFPVKIRGASAGWTRAVAIVED"
     gene            complement(1250..>1964)
                     /locus_tag="VGP50_00145"
     CDS             complement(1250..>1964)
                     /locus_tag="VGP50_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HEV8025800.1"
                     /translation="HAMDKDHNADLPILEAVAILNSYGLEVVSGIILGLDTDTAETPS
                     RILDFIERSQIPMLTVNLLQALPKTPLWDRLAAAGRLIDDPERESNVDFLLPYEQVVA
                     GWRRVVAATYRPEAIYRRFAYNVAHTYPNRIRLGADSARASFANMRKGARIVANLLWR
                     AGIAGNYRRSFWSMALPLLRSGRIEDMIHVGLVAHHLITFAQDAVAGRQNASFYSTKL
                     RGTEAKEEKTRLTTEAVRQ"
BASE COUNT          312 a          606 c          696 g          350 t
ORIGIN      
        1 atcgatgtgc gccccgacat cggcgagatg atgacgcaga tgggccgcgg ccgcgaagtg
       61 cgcgcggccc aggctttggc cccgtagcga gcatcgtgac gaggagatca gctcgggcac
      121 gccgcgtcgc cgctatcggc cgcgccggtg aggccgcgga ctcaagtcct tctccgcccc
      181 tcagggcgga gaaggagaat gacagccgag tcaatcctcg acgatcgcca ccgcgcgtgt
      241 ccagccggcg gaggcgccgc ggatcttcac ggggaagcag ctcaccgtga agccgtcggc
      301 cggaagcgcc tcgaggttgt gcagcttctc gagatggcaa tagccgatct cgcggccggc
      361 cttgtggcct tcccagatga ggcgtgcatc ctgggtggcg gtgtatttct cgcgggtgtg
      421 gacgaagggc gcgtcccagc tccaggcatc ggtgccggtg agacgcacgc cgcgctcgag
      481 aagatggagc gtcgccgcgc ggcccatgcc gcagccggcc gcgacatagt cgggcttgcc
      541 gtaagcggcg cccgcgcgcg tgttgacgac gacgatgtcg agcgggcgca gctcatggcc
      601 gatgcggtcg agttcgcgcg cgacgtcgtc tgccgtcact acataaccgt cggggagatg
      661 gcggaaatcg agcttgacgc cgggctgcag gcaccagtcg aggggcactt cgtcgatggt
      721 gatggcgcgc tcgccgcgat tcatggtgga gtggaaatga tagggcgcgt cgagatgcgt
      781 gccgttatgg gtcatgaggc ggacccattc gatggcccag ccctcgccat cgggcagatc
      841 ctcctccttg aggccgggaa agaagcgcgc gacgtcggcg gcggtgttct tgtgcgtcag
      901 atactcgatg cgcgggccat agcccggcgg gtcagacgcg acctcgttct cgagcggcat
      961 cgaaacgtcg atcaggcgcc gtgccatatt cccctccgct tcgccccggc cggagcatag
     1021 cgcaagccgc gacgggcgtc ctcgctcagg gcctgtcgtc ttggagcgca tctcggcaac
     1081 tggaaccgtt cctttgccgt catccccgcg aaagcgggga cccagggcta ccggcacgtc
     1141 gcacgccctg ggtccccgct ttcgcgggga tgacgatgtt gggaagtgtc gattccactt
     1201 gcgcgagatg tgctctacct ctgtgtctcc ggtgattctt tctttcttct cactgcctca
     1261 ctgcctctgt ggtcaatctt gttttttctt ccttcgcctc ggtaccgcgg agcttcgtcg
     1321 agtagaagga ggcgttctgg cggccggcga ccgcgtcttg cgcgaaggtg atgaggtgat
     1381 gcgcgacgag gccgacatgg atcatgtcct cgatgcgccc cgagcgcagc agcggcagcg
     1441 ccatcgacca gaagctccgg cggtagttgc cggcgatgcc ggcgcgccac agcagattgg
     1501 cgacgatccg cgcgcctttt cgcatattgg caaaagaggc gcgcgcggaa tcggccccga
     1561 ggcggatgcg gttcggatag gtgtgtgcga cgttgtaggc gaagcggcga tagatcgcct
     1621 cgggccgata ggtcgcggcg acgacgcggc gccagccggc caccacctgc tcgtagggca
     1681 ggaggaagtc gacattcgat tcccgctcgg ggtcgtcgat gagccgcccc gcggcggcga
     1741 ggcggtccca gagcggcgtc ttcggcaagg cctgcagcag attgacggtg agcatcggga
     1801 tctgcgagcg ctcgatgaaa tcgaggatgc gcgacggcgt ctcggccgtg tcggtgtcga
     1861 ggccgaggat gatgccggag accacttcga gaccgtagct gttcaagatg gcgaccgcct
     1921 cgaggatcgg gaggtcggcg ttgtggtcct tgtccatggc atgc
//
LOCUS       DASYNS010000005        13085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3121745, whole genome shotgun sequence.
ACCESSION   DASYNS010000005 DASYNS010000000
VERSION     DASYNS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3121745"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VGP50_00150"
     CDS             complement(<1..351)
                     /locus_tag="VGP50_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV8025801.1"
                     /translation="MRGLMMDRPLLIQSLIQYASEYHGDTEVVSRTVEGPIHRYTYTD
                     VERRSRQLAKALRRLGVKLGDRVGTIAWNGYRHFELYYGISGIGAICHTLNPRLFADQ
                     LRYIVNHAEDKVMFL"
     gene            complement(431..1201)
                     /locus_tag="VGP50_00155"
     CDS             complement(431..1201)
                     /locus_tag="VGP50_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8025802.1"
                     /translation="MDAPSPFSLSGKIALVTGAARGLGRVMAASLAEAGAHVLVNGRD
                     AGRLAPVVDELRQRGLLASPLVFDVADEGASDAAFAAIGRDFGRLDILVSNVGLRNRR
                     PIGALGRAEMRQMLEVNLTAAFGLAKAAAAMMLPRRQGRLILVTSVAGPLSRAGDAAY
                     TASKGGLAALTRALAVEFGPEGITANAVAPGFFATETNAALVGDPAMEALLERRVPLR
                     RWGNPAEIGGAVVFLASDAASYVNGHVLTVDGGFSSSY"
     gene            complement(1272..2213)
                     /locus_tag="VGP50_00160"
     CDS             complement(1272..2213)
                     /locus_tag="VGP50_00160"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV8025803.1"
                     /translation="MTKAITRVAVIGVGTIGASWAAYFLSRGLAVTASDPAPDAESFL
                     QSYIANAWPALERLGGMAASAGAGRLVFERDPLKAAAGAEFVQENGPEREELKIALFA
                     ALDAVLPPETVIASSSSGFLISKLQSRCRRPERCVLGHPFNPPHLIPLVEVVGGARTA
                     PEAVARTLAFYRAIGKQPIHIRKEVPGHVANRLQAALWREAVHLVATGVADVADIDAA
                     ITAGPGLRWPIFGPHLTFHLAGGSGGIAHFMSHLLAPMQSWWNDLGNPEMTPEVQRRI
                     IDGVAAEAAGRSIADLAAERDRCLVALLETLRSAKGR"
     gene            complement(2349..3692)
                     /locus_tag="VGP50_00165"
     CDS             complement(2349..3692)
                     /locus_tag="VGP50_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV8025804.1"
                     /translation="MTADLIFLLQAFVVVTIPFALSRALKLSGAVPLVVIQILLGIAL
                     GPSLFGRIAPDAYRLLFNPATLGPLTGTASIAVLFFSFITGLHVDTEIFRGRSRSFAA
                     IAAASVAVPTLLGIVGGLVIGLRHPGELGEHADLVRFSCAIGICVGVTALPVLAAILG
                     EMDLLGDRIGDLALGIAAVNDATLWLLLAGLMTLVAGGGKQRDILFLLLALPTYLLVM
                     VRVVKPLLAHAVAAIMRDGTMSEAVLVMIGAAAIGSAMVTQIIGLHYIFGAFLAGAIV
                     PRELRQTVVDRLQAMTINVLMPFFFMLTGLRILIIPSSAAFIEVLLVTTVLGMLGKVG
                     GTAATAMMMGERWRSALSLGALVQTKGLMEVIVLTILLEHGIISTTVFSALTVMAVIS
                     TALVMPSLRLLLPRTEEDRPRRPDIRDALRWTGKLAPAGIDSRAVTHPSRERDRH"
     gene            complement(3863..4303)
                     /locus_tag="VGP50_00170"
     CDS             complement(3863..4303)
                     /locus_tag="VGP50_00170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV8025805.1"
                     /translation="MTEDIAKPNIADADFHRHWTKDVVRYADTDRQGHVNNAVFATFI
                     ESGRVAILYDPAKPLAPAGASFVIARLLVDFLAEIRWPADVAIGTSVLRIGRSSVTLG
                     QGLFVDNRCVATAETVIVLMDDATRKSRPFPDETRAELMRWQHG"
     gene            complement(4436..5482)
                     /locus_tag="VGP50_00175"
     CDS             complement(4436..5482)
                     /locus_tag="VGP50_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025806.1"
                     /translation="MSDIPYRYITVVEKDDHGQAFTIDAVKKYSEGFSKPVLFRQMVD
                     IDPRLSTRAFFDEIDPGLSVQWRRKTGSAPATLRTADGKTDYNYVCGQLGSGSEFLDD
                     IFERKLDVYSHLGTISAGYKDPYEWGKVAFHKVREEIYRKNWFRIESWRTTGHMFLGH
                     GNEDYGEPIHGAVGSDWHMFPTLNVFVMIAGRKKWMTRPPELLEQLDGDQRMFSTSSG
                     REAPGEDFDHDTVFVDPGDVLVNMPYEWHKVLNAKGLSLGGAFRIIDGDYVGRLASRP
                     TVRSILPRFAEEVPEDVAHLLTSLKYASFHLNRAQMILNDAEHIYPNLLKAARPRDRA
                     AGPEAESPRIAVVQ"
     gene            complement(5714..6700)
                     /locus_tag="VGP50_00180"
     CDS             complement(5714..6700)
                     /locus_tag="VGP50_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV8025807.1"
                     /translation="MQSRYLRGVLFCLVATISWGCMFPVMTSALKRMDPFNFTAIRYS
                     IAALPFVVLLILREGLGALDLRGQRIFPAWLFGTAAFAGFNFLIFLGQQLAGPTGALN
                     AAIMTATMPLLSLPVNWVMRGIRPPWFSVGFIVLAFGGVVLVITKGNIAGALDQSSHV
                     ADIALFVGALCWVIYTTGGSLYPEWSPYRYTAITTLLGLASVYAINFILIEGGYIPAP
                     SLAQVYDLVPHLIYMSLVAGVIGVLFWNMGNNIITPMNGVLFMNVVPVAAFAVSALSG
                     ILPTPVQILGASITVAALVLNNLYQRQQLLKQGSVARPMVMGSATAPQPKFK"
     gene            complement(6728..9115)
                     /locus_tag="VGP50_00185"
     CDS             complement(6728..9115)
                     /locus_tag="VGP50_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV8025808.1"
                     /translation="MLERSTGFAGRLAGLLLADQGATVIIGRRDACSIPLDSFFDRGK
                     WRNSEHDAVAGGAIDLVIRDGTDTAARPIQQIRLAITAVLPGETAYPFPDSVGDDVLK
                     AALGFYTDLALTRRLLGEDVVYTPLALCSIYAGVLGALAAAAALADRARHGRGRDIVI
                     SRLAAGLSAIGALALEVDGMPAHLAPSALLRMPPELTVHLDDARASAERFEWLKRRMN
                     PFVACYPTADRRLVMMFAVAHRGIARRLLETLGAWDEMAAAGLVDLSPYDLANAAFAD
                     RNIGLPENLRLSLRVKAAELMETALARDNASHWERRFGDVGVACVVVRDFEEWRRLRQ
                     TADAGLVETVAGCDWPQLGRAVRLASAQPYPPLRAARDAAPETPVPAAPPAPEALPLQ
                     APLTGIRVLDLSNVIAGPACGRVLAELGAEVIKIDSPRPQHVPQVMAVFPGETAQGKR
                     SLLLDVASKSGREVLRRMVAGADVVVFNKLDPALERLGLDRSTFAAINPDAIGVQLSA
                     FKGERPSSRDDCTGYDPVLQAATGIMTRFGSGEGPELHGVASCVDYLTGYLAAFGAAV
                     ALAARVRRSGGGDWVETSLANAASLVQLSFQVSPPPVGSAECLRRTRDGWICVDPADA
                     MADDLANSPTEDALAAARSAGVVAAPVQSIAQLKARHRDSPSATVALRTHCGAFPAVN
                     LRPTWFQFDGALLPQTAPASLPGADGPAILDELGCGAAEIAGLIAEGVVGATLWGGAP
                     ASPFDVPPSSIGGRASPRLEHGGARRRRDDGDPPAGAPVLETAAVDDTEPHAADD"
     gene            9416..10111
                     /locus_tag="VGP50_00190"
     CDS             9416..10111
                     /locus_tag="VGP50_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8025809.1"
                     /translation="MVGVRQFDEAQVLTKALDVFWRKGLHATSMRDLAEATGVQRGSL
                     YHAYGDKERLFLLAFERYAARFLSEARSALDGTEAGSALLRFFDVAIANMRSGSPPRG
                     CLTTKMATEAALAGPRIKQRLRALLDELGIVVRDALARDPFRSALGLPPAEAAEVIVT
                     FTRGLAVMERLYQDQHRLKDSAAALVRALLAQRLATPHRSKRASPRRAARARSKLPAS
                     VPTPPSAPARGRR"
     gene            complement(10074..11273)
                     /gene="metZ"
                     /locus_tag="VGP50_00195"
     CDS             complement(10074..11273)
                     /gene="metZ"
                     /locus_tag="VGP50_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEV8025810.1"
                     /translation="MSPSFRPLPSGLRPRTLMTRAGMLRSEFMETCEAIYMNSGYVYA
                     TAEEAEAAFANDGSRFVYSRYANPTVAMFEERLRLLEGAEACRGTASGMAAVFAALAC
                     QVKAGDRVVASRALFNSCNYILTQVLPRYGVSTELVDGRDLEQWEKALAPGVKAVFCE
                     TPSNPTLELVDIAAVAELTHRAGGVLVVDNVFATPLLQRPLALGADIVVYSATKHIDG
                     QGRSLGGAVLGSESFVRESLVPFLRHTGPALSPFNAWLLLKGIETLDLRVERQSRSAA
                     EIAQALSRHAMVARVLYPGLESHPQYALARRQMSGGSTLVALSVEGGKAGAFRFQNAL
                     KLIEISNNLGDSKSLMTHPATTTHHRLKPEERALLGIDDGLVRLSVGLEDPADLIADL
                     EQALKAV"
     regulatory      complement(11346..11424)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            complement(11475..11717)
                     /locus_tag="VGP50_00200"
     CDS             complement(11475..11717)
                     /locus_tag="VGP50_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025811.1"
                     /translation="MTRTSMMMRWAWLAVAVVALSACSSSDSKTSASDSKTTVDPAAS
                     AAEEAARQQKDCADPEWKAAHLGVWYSVCRPNTGFR"
     gene            11879..12649
                     /locus_tag="VGP50_00205"
     CDS             11879..12649
                     /locus_tag="VGP50_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8025812.1"
                     /translation="MKLFDLSGKTAVITGSSKGIGRAIAEQMALFGANVVVSSRKAGP
                     CEEVVAGIKKAGGKAVSIPAHIGDKAALQNLVDKSRDAFGQIDILVCNAAVNPYFGPL
                     AEISDEQFDRIMDNNIRSNLWLCTMVMPEMAKRKDGAIIIVSSIGGLRGSAMLGAYAI
                     SKAADMQLARNLAVEWGQHNIRVNAIAPGLVKTDFARTLWENKDLLAATLRQSPLGRI
                     GEPVDIAGTAILLASNAGLFITGQTFVVDGGVTIAGRG"
     gene            complement(12917..>13085)
                     /locus_tag="VGP50_00210"
     CDS             complement(12917..>13085)
                     /locus_tag="VGP50_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV8025813.1"
                     /translation="NVDGGAISIGHPFGMSGSRLTGHALIEGKRRGAKHVVVTMCIGG
                     GMGAAGLFEIA"
BASE COUNT         2353 a         4284 c         4393 g         2055 t
ORIGIN      
        1 gaggaacatc accttgtcct cggcgtgatt gacgatgtag cgcagctgat cggcgaagag
       61 ccgcggattg agcgtatggc agatcgcgcc gatgccggag atgccgtaat agagctcgaa
      121 atgccggtat ccgttccagg cgatggtacc gacgcggtcg ccgagcttca cgcccaaacg
      181 ccgcagcgcc ttggcgagct gccgcgagcg ccgctcgacg tcggtgtagg tatagcggtg
      241 gatcggccct tcgaccgtgc ggctcaccac ctcggtgtcg ccgtgatatt cgctggcgta
      301 ttggatgagc gattggatga gcagcggacg gtccatcatc aagccgcgca tttttcctcc
      361 cggaagagcg ccgtttctgg ttcagccgca aggatatacc ggattccggc cgccggcgcg
      421 acgagacgcg tcaataggaa gacgagaagc cgccatcgac agtcagcacg tgaccattga
      481 cataggacgc cgcgtccgac gccaggaaca cgacggcgcc gccgatctcg gcgggatttc
      541 cccagcgccg gagcggcacg cggcgctcga gaagcgcctc catcgccggg tcgccgacca
      601 gggccgcgtt ggtctcggtg gcgaagaagc cgggcgcaac ggcgttggcc gttatgccct
      661 ccggcccgaa ctcgaccgcg agcgcgcgcg tcagggccgc gagcccgcct ttcgacgcgg
      721 tgtaggcggc gtcgccggcg cgcgatagcg gccccgcgac cgaggtgacg aggatcaacc
      781 gtccctggcg gcgcggcagc atcatcgccg cggcggcctt cgccaggccg aaggcagcgg
      841 tgaggttgac ctcgagcatc tgccgcatct ccgcgcgccc gagcgcgccg atgggacggc
      901 ggttgcgcag accgacattg ctgacgagga tgtcgagccg gccgaaatcg cgcccgatcg
      961 cggcgaaagc ggcgtccgag gctccttcat cggcgacgtc gaaaacgagc ggcgaggcca
     1021 agagaccgcg ctggcgcagc tcatcgacga ccggcgcgag ccgccccgcg tcgcggccat
     1081 tgaccagcac atgggcgccg gcctcggcca ggctcgccgc catgacgcgg cctagcccac
     1141 gcgccgcgcc ggtcacgagc gcgatcttgc cgctgagcga aaagggcgat ggcgcgtcca
     1201 tggcgcaatc ctgtcgtgct ccgggtcatc gaggcaaggg gacaagcggc gggcggcgac
     1261 cgacgcggcc gctacctacc cttcgcgctt ctgagcgttt caagaagcgc gaccaggcag
     1321 cggtcccgtt cggcggcgag gtcggcgatg gagcggcccg ccgcctcggc ggcgacgccg
     1381 tcgatgatcc ggcgctgcac ctccggcgtc atctcgggat tgccgagatc gttccaccag
     1441 ctctgcatcg gcgccagcag atggctcatg aaatgcgcga tgccgccgct cccgccggcc
     1501 aaatggaagg tgaggtgcgg cccgaagatc ggccagcgca ggccgggccc ggcggtgatg
     1561 gcggcatcga tgtcggcgac gtcggcaacg cccgtcgcca cgagatgcac ggcctcgcgc
     1621 cacagcgccg cctgcaagcg gttggcgacg tggcccggca cttccttccg gatgtggatc
     1681 ggctgcttgc cgatggcgcg gtagaaggcg agcgtccgcg ccaccgcctc gggcgcggtg
     1741 cgcgcgccgc cgacgacctc gaccagcgga atgagatgcg gcgggttgaa tggatggccg
     1801 agcacgcagc gctcggggcg gcggcagcgc gactggagct tcgagatgag aaagccggaa
     1861 gagctcgaag cgatcaccgt ctctggcggc aggacggcat ccagggcggc gaagagcgca
     1921 atcttgagct cctcgcgctc gggtccgttc tcttggacga attcggcgcc ggcggcggcc
     1981 ttcagaggat cacgctcgaa caccagccga ccggcgccgg cgctcgcagc catgccgccc
     2041 agccgctcga gcgcgggcca ggcattggcg atgtagctct gcaggaaact ctctgcgtcg
     2101 ggggccggat cgctcgccgt cacggcgagg ccgcgggaga gaaagtaggc ggcccagctc
     2161 gcgccgatcg tgccgacgcc gatcacggcg actctggtga tggccttggt catccgggtg
     2221 gcctctctgg gacaatggtc gaagccatta cccctcccgg ccctctttcc acaagcgccg
     2281 gcgacgaccg gcagccgatc aaggggcctc gctttggacg gcgcggattc cggcccgtgt
     2341 cgagagacct agtgccgatc gcgctctctt gaggggtgcg tcacggcccg actgtcgatc
     2401 ccggccgggg ccaacttccc agtccatcga agggcgtcgc gaatatcggg ccgacgcggc
     2461 cggtcctctt cggtacgcgg cagcagaagc cgcagcgagg gcatgacgag cgcggtgctg
     2521 atcaccgcca tgaccgttag cgccgaaaag accgtcgtcg agatgatgcc gtgctcgagc
     2581 agaatcgtga gcacgatcac ttccatcaag cccttggtct gaacgagcgc gccgaggctt
     2641 aaagccgaac gccatcgctc gcccatcatc atcgccgtcg ccgccgttcc acccaccttg
     2701 ccgagcatgc caagcaccgt ggtcaccagg agaacctcga tgaaggcggc ggaagaggga
     2761 atgatcagga tgcgcagccc cgtcagcatg aaaaagaagg gcatcagtac attgatcgtc
     2821 atggcctgga ggcgatcgac gacggtctga cgcaactccc gcggcacaat cgcgccggcg
     2881 aggaaggccc cgaagatgta gtggagcccg atgatctgcg tgaccatcgc ggagccgatg
     2941 gcggcggcgc cgatcatgac caaaaccgcc tcgctcatgg tgccgtcccg catgatagcc
     3001 gcgaccgcat gcgcgagaag cggcttcacc acacgcacca tgacgaggag ataggtcggg
     3061 agcgccagga ggaggaagag gatgtcgcgt tgctttcccc cgccggcgac caacgtcatg
     3121 agcccggcca gcagcagcca aagggtcgcg tcattaaccg ccgcaatacc caaggcgagg
     3181 tcgccgatcc gatcgccaag cagatccatt tcgccgagga tcgcggccaa gaccggcagc
     3241 gccgtgacgc cgacgcaaat gccaatggcg caggagaaac ggacgagatc ggcgtgctcg
     3301 ccaagctcgc cggggtgacg caggccgatg acgaggccgc cgacgatccc gagaagcgtg
     3361 ggaacagcga cgctcgccgc agcaatcgcc gcaaaggagc ggctgcggcc gcggaagatc
     3421 tcggtatcga catgcaggcc ggtgatgaag ctgaagaaga gcacggcgat cgacgcggtc
     3481 cccgtgagcg gcccgagggt cgccggattg aacaggagac gataggcatc gggcgcgatg
     3541 cgcccgaaga gcgaggggcc gagcgcgatg cccaggagga tctggatcac gacgagcggc
     3601 acggcgccgc tcagctttaa tgcccgcgac agcgcgaagg ggatcgtcac gaccacgaat
     3661 gcctgaagga ggaagatcag atcggcggtc atcggtcgat gcctgcaaac cgcagaaaca
     3721 cgtcgggcgt catgacaaca gtcattccat tgtccttccg gaatcggccc gctcgccata
     3781 gagcgcagcg gctgtccgat cattcagctg tggaacggca atcctgaccg gcggtcgctc
     3841 ggttcggcgc gaccatccgc gcttagccgt gctgccatcg catcagttcg gcgcgcgtct
     3901 catcgggaaa gggccgcgac ttgcgcgtgg cgtcgtccat gaggacgatc accgtctccg
     3961 ccgtcgcaac gcaacggttg tcgacgaaga gaccttgtcc gagcgtcacc gagcttcgcc
     4021 cgatgcgcag gaccgaggtg ccgatggcga cgtcggcggg ccaccggatc tcggcgagga
     4081 aatcgacgag aaggcgcgcg atgacgaaag acgctcctgc cggcgccaat ggcttggccg
     4141 gatcgtagag aatggcgacc cgtccggatt cgatgaaggt cgcgaacacg gcattgttca
     4201 catgcccctg gcgatccgtg tcggcgtaac gcacaacgtc cttggtccaa tggcgatgga
     4261 aatcggcgtc ggcgatgttc ggcttggcga tgtcttcggt cactgcggac atagttcctc
     4321 accctcgttc tgcgggagct cggcgcgcgc aattcccacg ccgcgatgcg ccagaccgcg
     4381 cacgccaagc tccggccgaa gcgccggcgc ccgctccggg cggccggccc cgacgtcact
     4441 gcaccacggc gatacgagga ctttccgcct ccggcccagc ggcccgatcg cgcgggcggg
     4501 cggctttcag caggttcgga tagatgtgct cggcatcgtt cagtatcatc tgcgcccggt
     4561 tgagatggaa ggacgcatat ttcaggctgg tgagcagatg cgcgacatcc tccggcacct
     4621 cttcggcgaa gcgcggcaga atcgacctga ccgtcggccg gctcgccaac cgcccgacat
     4681 agtcgccgtc gatgatgcgg aacgcgccgc cgagactgag ccccttcgcg ttcagcacct
     4741 tgtgccactc gtacggcatg ttgacgagca cgtcgcccgg atcgacgaag acggtatcgt
     4801 ggtcgaagtc ctcgccgggc gcctcgcgtc cgctgctcgt cgagaacatc cgctgatcgc
     4861 cgtcgagctg ctcgagaagc tcgggtgggc gcgtcatcca cttcttgcgt ccggcaatca
     4921 tcacgaagac gttgagcgtc ggaaacatgt gccagtcgct gccgacggcg ccatggatcg
     4981 gctcgccata gtcttcgttg ccgtggccca agaacatgtg acctgtcgtc cgccaggact
     5041 cgatacggaa ccagttcttg cggtagatct cctcgcgcac tttgtggaag gcgaccttgc
     5101 cccattcata gggatcctta tagccggccg atatggttcc gagatgcgag tagacatcga
     5161 gcttgcgctc gaagatgtcg tcgaggaatt cgctgccgct gccgagctgg ccgcatacgt
     5221 aattgtagtc ggtcttgccg tccgccgtgc ggagcgtggc cggggcgctt ccggttttgc
     5281 gccgccattg aacgctcaaa cctgggtcga tctcgtcgaa gaaggcgcgc gtcgacaatc
     5341 gtggatcgat gtcgaccatc tggcggaaga gcacgggctt cgagaagcct tccgagtatt
     5401 tcttgacagc gtcgatggta aatgcctgac cgtgatcatc cttttcgacg acggtgatat
     5461 atcgatatgg gatgtcgctc atcattattc ctctgtccat gtggcttcac gacgtccact
     5521 tgcgcgccgc ctccgttctt ccttctttta ttttggtttt tcagtttatg ttgcactgca
     5581 gaatagcgac ggtctttgac gggtcaaccc cacattctcg cataaagggt atgaaaaact
     5641 tgcagactat ttttcaatgt gcggcgcggc ctcgccccca ggcgggcgcg atcggagctc
     5701 gaagcggagg cgttcatttg aatttgggct gcggcgccgt tgcgctgccc atcaccatcg
     5761 gccgcgcgac gcttccctgc ttgagcaatt gctggcgctg gtacagattg ttgagcacga
     5821 gcgcggcgac cgtgatcgac gcgcccagga tttgcaccgg cgtcggaaga atgccggaga
     5881 gcgccgacac ggcgaaagcc gcgacgggga cgacattcat gaacagcacg ccattcatcg
     5941 gcgtaatgat gttgtttccc atgttccaga agagcacgcc gatgacgccg gcgacgagcg
     6001 acatgtagat caggtgcggc acgaggtcgt agacctgcgc gagggatggt gccgggatgt
     6061 agccaccttc gatgaggatg aaattgatcg catagacgct ggcgaggccg agcaaggtcg
     6121 tgatcgccgt gtagcggtag ggcgaccatt ccgggtagag gctgccgccc gtcgtataga
     6181 tgacccagca gagcgcaccg acaaacagcg cgatgtcggc gacgtggctc gactgatcga
     6241 gtgcgccggc gatgtttccc ttggtgatca ccagcacgac accgccgaag gccagcacga
     6301 tgaagccgac cgagaaccag ggcggccgga tgccgcgcat cacccagttc accggcagcg
     6361 acagcaacgg catcgtcgcc gtcatgatcg cggcgttgag cgcacccgtg ggacccgcaa
     6421 gctgctgtcc cagaaagatc aggaaattga agccagcgaa ggccgccgtg ccgaacagcc
     6481 aggccgggaa gatccgctgt cccctgagat cgagcgcgcc taggccctcg cgaaggatca
     6541 gcaggacgac gaagggcagc gcggcgatgc tgtagcggat ggccgtgaaa ttgaaggggt
     6601 ccatgcgctt cagcgccgac gtcatgaccg gaaacatgca accccacgag atggtcgcga
     6661 cgagacagaa cagcacgccg cgaagatatc tgctctgcat cgggtccctc tctacgccgg
     6721 cgtcatgtca gtcatcggcg gcatgcggct ctgtgtcgtc gacggcggcc gtctcgagga
     6781 cgggcgcgcc ggcgggcgga tcgccgtcat ctcgccggcg ccgcgcgccg ccatgttcga
     6841 ggcgcggcga ggcgcggccg ccgatggacg acggcggaac atcgaagggc gatgccggcg
     6901 ccccacccca gagtgtcgcg cccaccacgc cctcggcgat caggccggcg atctcggccg
     6961 cgccgcagcc gagctcatcg agaatggccg gcccatcggc gcccggcagt gaggccggcg
     7021 ccgtctgcgg cagcaacgct ccgtcgaact ggaaccaagt cggccgtaga ttgaccgccg
     7081 ggaaagcgcc gcagtgcgtg cgcagcgcga cggtggcgct cgggctgtcg cgatgtctcg
     7141 ccttgagctg agcgatcgac tgcaccgggg cggcgacgac gccggccgac cgcgcggcgg
     7201 cgagcgcgtc ttccgtcggc gaatttgcaa gatcgtccgc catcgcgtcg gccgggtcga
     7261 cgcagatcca gccgtcccgc gtccggcgca ggcattcggc gctgcccacg ggcggcggcg
     7321 atacctgaaa agacaattgc acgaggctgg cagcattggc caaggaggtc tcgacccagt
     7381 cgccaccgcc ggaacgacgc acccgtgccg ccagcgcgac ggccgcgccg aacgccgcga
     7441 gatagccggt gagatagtcg acgcaggagg cgacaccgtg cagctcggga ccttcgccac
     7501 tgccgaagcg cgtcatgatg ccggttgccg cctgcagcac cgggtcatag ccggtgcaat
     7561 cgtcgcggct gctcggccgc tcgcccttga aagcgctgag ctgcacgccg atggcgtcgg
     7621 gattgatcgc ggcgaaagtc gatcgatcga ggcccagccg ctccagcgcc ggatccagct
     7681 tgttgaagac gacgacgtcg gcgcccgcca ccattcgccg caacacctcg cgaccgctct
     7741 tggaggcgac gtcgagcagg agactgcgct tgccctgggc ggtctcgccc gggaagactg
     7801 ccatcacctg cggcacatgc tggggacgcg gcgaatcgat cttgatcacc tccgcgccga
     7861 gctcggcgag cacacggccg caagcgggcc cggcgatgac attgctgaga tcgagcacgc
     7921 gaatgccggt gagcggtgcc tgcaacggca gcgcctcggg cgcgggcgga gcagcgggca
     7981 ccggggtttc cggcgcggcg tcccgcgccg cccgaagcgg cggataaggc tgcgcgctcg
     8041 ccagccgcac ggcgcggccg agctgcggcc agtcgcaacc ggcgaccgtc tcgaccaatc
     8101 cggcatcggc ggtctgacgc aagcgccgcc actcttcgaa gtcgcggacg acgacgcagg
     8161 cgacgccgac atcgccgaag cgccgctccc aatgactggc gttgtcgcgc gccaaggcgg
     8221 tctccatcaa ctccgctgcc ttgacgcgta gcgacagccg taaattctcc ggcaagccga
     8281 tgttacgatc ggcaaaggcc gcattggcca gatcataggg cgataaatcg acgagcccgg
     8341 ccgctgccat ctcgtcccag gcgcctagtg tctcgagcag gcggcgagcg atgccgcgat
     8401 gagcgaccgc gaacatcatc accagacggc ggtcggcggt cgggtagcag gcgacaaacg
     8461 gattcatgcg ccgcttgagc cattcgaagc gctcggcgct ggcccgcgcg tcatcgagat
     8521 gcacggtcaa ttccggcggc atccgcaaca aggcgctcgg cgccagatgc gcgggcatcc
     8581 cgtcgacttc gagcgcgagc gcgccgatcg ccgagagtcc ggcagcaaga cgcgagatca
     8641 cgatgtcgcg acctcggccg tggcgcgcac ggtcggccaa agccgccgct gcggccagcg
     8701 cgccgagcac gccggcgtag atggagcaga gcgcgagcgg ggtgtagacg acgtcctcgc
     8761 ccagcaatcg ccgggtcagg gctaggtcgg tatagaagcc caatgctgcc ttgaggacgt
     8821 catcgccaac ggaatccgga aaaggatagg cggtctcgcc cggcaatacc gcggtgatgg
     8881 caaggcgaat ttgctgaatc ggccgcgccg cggtgtcggt gccgtcgcgt atcaccagat
     8941 cgatggcgcc gccggcgacg gcgtcatgct cagagtttcg ccatttcccg cgatcgaaga
     9001 acgagtcgag cggtatcgag catgcgtcgc ggcggccgat gatgacggtg gcgccctgat
     9061 cggcgaggag cagcccggcc aagcgaccgg cgaatccggt gctccgctcg agcacgcaaa
     9121 ccccctccaa tggcaatcgc gccatcttcc accttcgctg ttgaagcgga tcccaacatc
     9181 tctgcgaaga gcataagatt cagcggatgt gtgccctcgc tcggtgatca ctcctggtcg
     9241 cagtctgccg cagcgtgccg gaaagtgcgt cggtcgaccc ccgtcgcgtc tcggagctgt
     9301 gcggcttatg caggcaatgg tgtttttctc gccacccgca cctcgctccg ttatagtggg
     9361 cgatgctatc atcattgacg atgttgtcaa tcatgtttcg aggcccgtat atcccatggt
     9421 cggcgtgcgt cagttcgatg aagcgcaggt tttgacgaag gcgctcgatg tgttttggcg
     9481 aaagggattg catgcaacct cgatgcgcga cctcgccgag gcgacgggcg tgcaacgcgg
     9541 ctcgctttac cacgcctatg gcgacaagga gcggctgttc ctcctcgcct tcgaacgcta
     9601 cgcggcacgc ttcctgagcg aggcgcgtag cgcgctcgat ggcacggagg ccgggagcgc
     9661 cctccttcgc ttcttcgacg tcgcgatcgc caacatgcgc agcgggtcgc cgccacgagg
     9721 atgtctgacg acgaagatgg cgacggaggc ggcgctggcc ggaccacgga tcaagcagcg
     9781 gctgcgcgcg cttctcgacg agctcggcat cgtcgtccgc gacgcactcg cccgcgaccc
     9841 gtttcgatcc gctttggggc tgccgcccgc cgaggctgcc gaagtgatcg tgaccttcac
     9901 gcgcggcctc gccgtgatgg agcggctcta ccaggaccag catcgcctca aagacagtgc
     9961 ggcagcgctc gttcgggcgc tgctggcaca gcgactggcg accccgcacc gctcgaaacg
    10021 cgcgtcaccg cgccgtgccg cccgcgcacg ctccaagctg ccggcaagcg tccctacacc
    10081 gccttcagcg cctgctcgag gtcggcgatg aggtcggccg ggtcctcgag gccgaccgag
    10141 aggcgcacga gcccgtcatc gatgccgagc aatgctcgct cctcgggctt caggcgatga
    10201 tgggtggtcg tcgcgggatg ggtcatgagg ctcttcgaat cgccgagatt gttggagatc
    10261 tcgatgagct tcagcgcgtt ctggaagcgg aaggcgccgg ccttgcctcc ctccaccgag
    10321 agcgccacca acgtgctgcc gccgctcatc tggcggcgcg ccagcgcata ttgcggatgg
    10381 ctctcgagac cgggatagag cacgcgcgcg accatggcat ggcgcgagag cgcctgcgcg
    10441 atctcggcgg ccgagcggct ttgccgctcg acccgcaaat cgagagtctc gatgcccttg
    10501 agcagcagcc aggcattgaa ggggctgagc gcggggccgg tatggcgcag gaacgggacc
    10561 aggctctcgc gcacgaagct ctcggagccc agcaccgcgc cgccgaggct gcgcccttgc
    10621 ccgtcgatgt gcttggtggc ggaatagacg acgatgtcgg cgccgagcgc cagcgggcgc
    10681 tgcaagagcg gcgtggcgaa gacgttgtcg acgacgagaa cgccgcccgc gcgatgggtg
    10741 agctcggcca cggcggcgat gtcgaccagc tcgagcgtcg gattggaggg ggtctcgcag
    10801 aacaccgcct tgacgcccgg cgccagcgcc ttttcccatt gctcgaggtc gcggccgtcg
    10861 acgagttcgg tcgagacgcc atagcggggc aggacctgcg tcaggatgta gttgcaggaa
    10921 ttgaagagcg cgcgggacgc cacgacgcgg tcgcccgcct tgacctgaca ggcgagtgcc
    10981 gcgaacacgg ccgccatgcc gctcgcggtg ccgcggcagg cctcggcgcc ttcgagcaga
    11041 cgcagccgct cctcgaacat ggcgacggtg ggattggcgt agcgcgagta gacgaagcgc
    11101 gagccgtcat tggcaaacgc cgcttccgct tcctcggccg tcgcgtagac atagcccgaa
    11161 ttcatataga tcgcttcgca ggtctccatg aactcgctgc gcagcattcc cgcgcgcgtc
    11221 atgagcgtgc gcggacggag cccggaggga agcggtcgga aggacgggga catgcggtct
    11281 gctcctcggt gtcgcgcttg tggcgcggac aagaaaaaac cctggatccg caaaaggcca
    11341 gggttcccgc aggagctccg accttttagc gtttgtttta cgtggccgca agccggtcgg
    11401 ctcaaattcg ccacgatagc cacagtcgag taccgcgccc ggcggccgct gtcaagcgcg
    11461 ccgcgggccc gccctcaccg gaaaccggtg ttggggcggc agacgctgta ccagacgccg
    11521 agatgcgccg ccttccattc gggatcggcg caatccttct gctggcgggc cgcctcctcg
    11581 gcggcagaag cggccgggtc gacggtcgtc ttgctatcag aggcgctcgt cttgctatca
    11641 gaagaggagc aggcggaaag agcaacgacc gctaccgcga gccacgccca gcgcatcatc
    11701 atcgacgtcc ttgtcatgca tcatctcccc gcgaaactac ccgaccccag ctaaagtttc
    11761 ttgatccata gcgccgcatc cgaaggcctc agggtaatgg cgcggggcgc ggggatcgag
    11821 tagtttgcgg aggagccggc cggacgccgg tcagggacgg aagaaaacgg ggacaggcat
    11881 gaagctcttc gatctctcgg gcaagaccgc cgtcatcacg ggctcctcca agggcatcgg
    11941 ccgggcgatc gccgagcaga tggcactctt cggcgccaat gtggtggtgt cgagccgcaa
    12001 ggcgggaccc tgcgaggaag tggtcgcggg catcaagaaa gccggcggca aggcggtgag
    12061 cattcccgcc catatcggcg acaaggcggc gctccagaac ctcgtcgaca agtcgcggga
    12121 cgccttcggg cagatcgaca tcctcgtctg caatgccgcg gtgaacccgt atttcggccc
    12181 gctcgccgag atctcggacg agcagttcga ccgcatcatg gacaacaaca tccgcagcaa
    12241 cctctggctc tgcaccatgg tcatgccgga gatggcgaag cggaaggacg gcgccatcat
    12301 catcgtctcg agcatcggcg gcctgcgcgg cagcgccatg ctcggcgcct acgcgatctc
    12361 gaaggccgcc gacatgcagc tcgcccgcaa tctcgccgtc gaatggggcc agcacaacat
    12421 ccgcgtcaac gccatcgccc ccggcctcgt caagacggat ttcgcccgca ccctctggga
    12481 gaacaaggat ttgctggcgg cgacgctgag gcaatcgccg ctcggcagga tcggcgagcc
    12541 cgtcgacatc gccggcacgg ccattctgct ggcatcgaat gccggcctct tcatcaccgg
    12601 ccagaccttc gtcgtcgatg gcggcgtgac gatcgcgggg cggggataaa gcgcatcccg
    12661 gtcacctgga accgttcctt cgtcgtcatc cccgcgaaag cggggatcca ggggctaccg
    12721 acacgtcgct tgccctggat ccccgctttc gcggggatga cgatgttagg aagtaccgat
    12781 tccacttgct cgagatctgc tctagccatc ggccgtcagc tcgtcgcaat gatggttgat
    12841 ggcttcggtg cgggctcccg cggttgcaag agcaaccgct ggcgcccgcc cgcgacgacg
    12901 cttcgcgtcg cgcggcttac gcgatctcga acagccccgc ggcgcccatg ccgccgccga
    12961 tgcacatggt gacgacgacg tgcttggcgc cgcggcgctt gccttcgatg agggcatggc
    13021 cggtgagccg cgagccgctc atgccaaagg gatggccgat cgagatggcg ccgccatcga
    13081 cgttg
//
LOCUS       DASYNS010000006         9811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1094047, whole genome shotgun sequence.
ACCESSION   DASYNS010000006 DASYNS010000000
VERSION     DASYNS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094047"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2329
                     /locus_tag="VGP50_00215"
     CDS             <1..2329
                     /locus_tag="VGP50_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025814.1"
                     /translation="PIGIAENELTQEITGGARSALTQRLVIRLFEKRLGERPFILVFD
                     DAHWFDDASAALLRQLVHAMPALAVVIASRPPGKGASSAFVPHLGDRGERGITLGALE
                     RRAVERLVDLKLGITAAPAPLYDFVFRRAAGHPLYTEELVLGLRAGGSIEITGAACRL
                     ATDSLENLELAVPNTLQGVIVSRVDRLDPAEQFVLKVASVTGTRFSAAMIAEVLPVPH
                     DRARLEGILERLAAEDFIACEDAATGAYGFKHVLVQQAIYDLLLFEQRQLLHRRVADW
                     LEAHAPGGPEAACTPLALHWERAGHAGNAVRYLEMAAGLALRRYSIGDAIAHGLRALT
                     LTDERRLLLPAARRARLSVILGDAYHELFDYEKAAHRFEEALAYLSWPIPKSRFGLAL
                     RTAAHAAAQLGRRLGLFGAARAAGAARACEAAHIYERLAEIAYFDSRSLELLHCTLAS
                     LNHAEASNSTREIVDGYAALSIGLGTAGLRRPAAFYNRRSLALAEAQGAMSDIAYAQL
                     VNMVFAASQGLWDRVEGAAARASPLFLQLGATIRWQQVQATLCFARLTRGDLDGAVPL
                     IAELKRSLENANTPAQVHAWTLGAELIVEIAHDRCVAALARSADAMALDPTIHRADRL
                     LCHGLAALAHLRNGDAARAAATADLALANLTESLPSAWHVTHAVAGLAETYLELGAPR
                     TKSGMEAKARAACDALRGFARRIPVATPSALLLRSRHAALTGRLRQARRLRRRALVAS
                     QRYGIALAAPPRNAIGDEAARLDPVEVGKTGDAFG"
     gene            2342..4483
                     /locus_tag="VGP50_00220"
     CDS             2342..4483
                     /locus_tag="VGP50_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV8025815.1"
                     /translation="MARNIVIIGGGMAGLTAAYELTKTPELRQRHKVTVYQLGWRLGG
                     KCATGRDERGRIREHGLHLWFGCYDNAFAMLKEVYKQWQRSPDNPLTDWKSGFASVDD
                     TPIGWLEDGQQRFVPITWPGNPAEPGDGNVALSVADLVVELGNLLKLLIGQIAPGDAP
                     ATVAVEAAAQEHLGAALDIGNIDGAICDKIAEGWLSGLHDIVEVVRLSAETLARAAPA
                     VAESHFPGVDWAMNCLRRAIARDAAGHFSTSPATRLIGDALDVGGAVLRGLFVDLLLT
                     GKGGDSLGNVEFRAWLKRHGASATVVDQSRVVRALYDTCFWYDGGDPESPSVDAATAL
                     CVLLRIVCTYRGAVAYKMRAGMGEVVVAPLYEVLKQNGVAFSFFRKLERIELDPTKKR
                     VAAIHFACQAQPKDADYVPTFRLNGFICWPSEPFWEQLRDGEQLKAKGVDFESPWESE
                     PPAGHETVTIAADDKVVLAVSLGAYKKLNSDPGPCDELLEADGRLRRMSEQIGLVPTQ
                     ALQLWSRDSMAALGWEGAPATVAGAKMLDVWADMSQTLHAEDWRDEPAPPLSVHYLCG
                     TYKTRLFAEPASAGDVMARASVELTASMGDWLDAFGTAIWPKATTPGAAFDWCVLWDS
                     ANGDGRQRLQAQYIRANIAPSECCPGSSAGSLPFRLRSDETAFANLVLAGCYLRTGLN
                     STCVEGAVMSGMQASRAICGSPDKILAEHFL"
     gene            4519..5499
                     /locus_tag="VGP50_00225"
     CDS             4519..5499
                     /locus_tag="VGP50_00225"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEV8025816.1"
                     /translation="MKDLPAYISDRGHGEICYEVPLSISGATVYAFALHARHEALQAL
                     VDSQLNAAASPTVHYEAVGHSVLLIYFHAEHSTSTTETIGWLPDRESMVWVLVVQKTA
                     QGSRLMFWIPYLLIDAMSGMVTGREVWGYRKSLATIDLPIAATDPAQFVARTVVFKTF
                     SPDTQGLVEPLITVSRNGPLGPLQTAWRDPEDVVKGAIAMLRESFSEFDMSDWLNAAT
                     VIEGLGKIAATLRIPVINLKQFRDVGDSAKACYQALVESPCELVTLHGAGPLPGDFTA
                     SFVTCDSHRVVADLGLGTPAPGATSVPVRFAFWWKMDFKTLDGSVVWQAS"
     gene            complement(5516..7630)
                     /locus_tag="VGP50_00230"
     CDS             complement(5516..7630)
                     /locus_tag="VGP50_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV8025817.1"
                     /translation="MSAEKTKVAILGGGPGGVAAAFWLSSSQELRDRFEVTLYTQGWR
                     LGGKCASARNYSAGERIEEHGLHMFMGFYRTAFATMRAVYPLTRRPDDRFRSWTDAFT
                     PQRLVSLGFAVPDGKGGTTWSPWTIEFPELPGNPGDLDDTGLDYLARAPKCLIAALRS
                     SVWPLLHDLLGRRVAGSVIKIDFFSMLHVDGALAETTVLFEERFAGVSTEIAGFFRKL
                     YAECSLETAWLLGERLPADHSVASDLLRELRRLQECFNTFLVATLRSCEEWGFHLYVM
                     ISLGFAGLIGYLEDVLPKGDAGFDALNALDFRAWLKSHGAPDDALGPGPVQAIYDLAF
                     AYEAGEAGKPGAAKMAAGAALRLVMRMCFGYRNAPLWRMQAGAGDVIFTPLYRYLAGR
                     HVAINFFHRVRDLGVKDGLVETIEIDRQLDVKNGRYLPLQEGDDRWPAEPLWDQIVDG
                     DVLGTKGLALESPWCTFKVGEQTLRRGQDFDVVVLAMPPLALKPISAQLAASVPWRNM
                     LDNMTAVPTQSVQLWMKPDLSGLGWKNGQSVLTAYAQDLSSWADMSQVREGGAGDPGS
                     CQYLCGVYHCAAPLPVGENPTFIDAQTKSVVQRTEAWLDENAGVLWPGVCPNGKALDR
                     SVVTDRYYRVNIGYSDQYVQTFPGSVQYRLDPGKSGFANVFLAGDWTLSSINGGSVES
                     AFESGQRAAEAITQKPSPAQPD"
     gene            8148..>9811
                     /locus_tag="VGP50_00235"
     CDS             8148..>9811
                     /locus_tag="VGP50_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV8025818.1"
                     /translation="MFSDSFSWLHLTDFHYGMKGQDCLWPNLREPFLESLAVLHEKCG
                     PWQAVLFTGDLVQSGESDQYKKMQDEVLAPLWRRLDELGSKNVALLAVPGNHDLYRPK
                     PDSPTFDDPAMETLLQPEGFNRIGAKFWTNSSGAYRRAIREVFAAYNEWWSRAPGRPP
                     NVKDGALPGDFSVSIEHGKRKIGIVGLNTAFLQLGIDDYAGRLTWNAQQLHAVCDGGI
                     DLWIRQHDVCLLLTHHGPNWLTPQAREHGAAEIAPPGRFAAQFFGHQHETSIEYIRRG
                     GGRAAIRLCQGCSAFGLDRFGDPPTTKRTHGYAAGRIDFGRGMATLRLWPRVATRGTG
                     PWRFIPDHEHAVLDPDEGTEAEEVAASSRAPRAPPAPTISGADSFIPHSTLPARRPFF
                     GRKAGLEKIAKYLLPDDRSWGVVLDGPGGVGKTSLAIEAAHGAPAEHFPLKLWITAKS
                     RELHPEGESRLTDHRVDDYYGLLNELGLSLGREDIPRAPPEERPALIRHALTQHKALL
                     VVDNLESFHTEDRRRLFELMGSLPTTCRAIVTSRRRTDGSLAAHMIR"
BASE COUNT         1707 a         3271 c         3134 g         1699 t
ORIGIN      
        1 gccgatcggc atcgccgaga acgagctgac ccaggagatc accggcggcg cgcgctcggc
       61 gctcacccag cggctggtga tccggctctt cgagaaaagg ctcggggagc ggcccttcat
      121 cctcgtcttc gacgacgcac attggttcga cgatgcctcg gccgccctgc ttcgacagct
      181 cgtgcacgcc atgcccgcgc tcgccgtcgt catcgcgtcg cggccgccgg gcaaaggcgc
      241 atccagcgcc ttcgtcccgc acctgggcga caggggcgag cgggggatca cgctcggcgc
      301 gctcgagcgc cgcgccgtcg agcgcctcgt cgacctgaag ctcggcatca ccgccgcgcc
      361 cgcgccgctt tacgacttcg tcttccgccg cgccgcgggc catcccctct ataccgagga
      421 gctggtactg gggctgcgcg ccggtggctc gatcgagatc acgggcgccg catgccgcct
      481 cgccacggac tcgctcgaga atctggagct ggctgtcccg aatacgcttc agggcgtcat
      541 cgtcagccgc gtcgaccggc tcgacccggc cgagcaattc gtcctcaagg tcgcgagcgt
      601 caccggcacc cgcttctcgg cggcgatgat cgccgaagtg cttccggtgc cgcatgaccg
      661 cgcacgcctc gaggggatat tagagcgcct cgcggccgag gatttcatcg cgtgcgagga
      721 tgccgcgacc ggcgcttatg gcttcaagca tgtgctggtg cagcaggcga tctacgacct
      781 cctcctcttc gagcaacgcc aacttctcca tcgacgcgtc gccgactggc tcgaagccca
      841 cgcaccgggc gggccagagg ccgcctgcac gccgctcgcg ctgcattggg agcgggccgg
      901 ccacgccggg aatgccgtgc gctatctcga aatggccgct ggcctcgcgc tgcgccgcta
      961 ttcgatcggc gacgcgatcg cccacggcct cagggcgctg accctcaccg acgagcgccg
     1021 gctcctgctg ccggcggcgc gtcgcgcacg gctctcggtg atcctcggcg atgcctatca
     1081 cgagctcttc gactacgaga aggcggcgca ccgtttcgag gaagcgctgg cctatctctc
     1141 ctggcccatt ccaaagagcc ggttcggcct cgcgctccgg acggcggccc atgccgcggc
     1201 tcagctcggg cgacggctgg ggctgttcgg cgccgcccgc gcggccggcg ccgcccgcgc
     1261 ctgcgaagcc gcgcatatct acgagcgcct ggcggagatc gcctatttcg acagccgcag
     1321 tctcgagctg cttcactgca cgctcgcatc gctcaaccac gccgaggcgt cgaactcgac
     1381 gcgcgagatt gtcgacggct acgcggcgct ttcgatcgga ttgggcacag ccggcctgcg
     1441 tcgccctgcc gctttctaca atcgtcgctc cctcgctctg gccgaagccc agggagcgat
     1501 gtcggacatc gcctatgcgc agctcgtcaa catggtcttc gccgcctcgc agggcctctg
     1561 ggaccgtgtc gagggcgcgg cagcgcgcgc ctcgccgctg ttcctccagc ttggcgccac
     1621 catacgctgg caacaggttc aggcgacgct gtgttttgcg cggctgacgc gcggcgatct
     1681 cgacggcgcc gtcccgttga tcgccgagct caagcgctcg ctcgaaaatg cgaacacgcc
     1741 tgcccaggtg catgcctgga ccctcggcgc cgagctgatc gtcgagatcg cgcatgaccg
     1801 ctgcgtcgct gctctcgccc gctccgccga cgcgatggcg ctcgacccga cgatccatcg
     1861 cgccgaccga ttgctctgcc acggccttgc cgcgctcgct catctgcgga atggcgatgc
     1921 agcacgcgcc gccgcgacgg cggatctggc gcttgccaat ctcaccgaga gcctgccatc
     1981 ggcgtggcac gtcacccacg ccgtcgccgg gcttgccgag acctatcttg agctcggcgc
     2041 gccgcgaacg aagagcggga tggaggcgaa ggcgcgcgcc gcctgcgacg cgcttcgcgg
     2101 ctttgcacgg cgcattccgg tcgcgacgcc ttccgcgctc ctcctgcgat cgcgccacgc
     2161 agcgcttacg ggccgcttga ggcaagcgcg acgccttcgc cgccgggcgc tcgtggcttc
     2221 gcagcgctac ggcatcgccc tcgccgcgcc accgcgtaac gcgatcggcg atgaggccgc
     2281 gcgcctcgat ccggtcgagg tcggcaaaac tggcgacgcc ttcggctgac gcaagcggtc
     2341 catggcacgg aacatcgtga tcattggcgg cggcatggcg gggctgaccg ccgcctatga
     2401 gctaacgaag acgccggagc tgcggcagcg tcacaaggtg acggtctatc agctcggctg
     2461 gcggctcggc ggcaaatgcg cgaccggccg cgacgagcgc gggcgaatcc gtgagcacgg
     2521 gctgcatctc tggttcggct gctacgacaa cgccttcgcc atgctgaaag aggtgtacaa
     2581 gcagtggcag cgcagcccgg acaatccgct caccgactgg aagagcggct tcgcgagcgt
     2641 cgatgatacg ccaatcggct ggctcgagga cggtcagcag cgcttcgtgc cgattacctg
     2701 gcctggcaat ccggccgaac ccggcgatgg caatgtcgcg ctcagcgtcg ccgatctcgt
     2761 cgtcgagctc ggcaacttgc tgaagctgct catcggacag atcgcgcctg gcgacgcgcc
     2821 cgccaccgtt gccgtcgaag cggccgcgca agagcatctc ggcgccgcgc tcgatatcgg
     2881 caatatcgat ggcgcgatct gcgacaagat cgccgagggt tggctctccg ggctgcatga
     2941 catcgtcgag gtggtgcggc tttcggccga gaccctcgcc cgcgcggcgc cggcggtcgc
     3001 ggagagtcac ttccccggag tcgactgggc gatgaattgc ctgcgccggg cgatcgctcg
     3061 cgacgcagcc ggccatttct cgacgagccc cgccacgcga ctgatcggcg acgccctcga
     3121 tgtcggcggc gccgtgctgc gcggcctctt tgtggacctg ctgctcaccg gcaagggggg
     3181 cgattccctg ggcaatgtcg agttccgcgc ctggctcaag cgccacggcg cctcggcgac
     3241 ggtggtcgac caatcgcgcg tcgtgcgcgc gctttacgat acctgcttct ggtacgacgg
     3301 aggcgatccc gagagcccga gtgtcgatgc ggcgacggcg ctctgtgtcc tcttgcgcat
     3361 cgtatgcact tacagaggcg ccgtcgccta caagatgcga gccggcatgg gcgaggtcgt
     3421 cgtcgcgccg ctctatgaag tgctgaagca gaacggcgtc gccttctcct ttttccgcaa
     3481 gctcgagcgg atcgagctcg atccgacgaa aaaacgcgtg gcggcgatcc attttgcctg
     3541 ccaggcgcaa ccgaaagacg ccgattacgt cccgaccttc agattgaacg gcttcatttg
     3601 ctggccgagc gagcccttct gggagcagct ccgcgacggc gagcagctta aggcaaaggg
     3661 cgtcgatttc gaatcgccct gggaaagcga gccgcccgca gggcacgaga ccgtgacgat
     3721 cgcggccgac gacaaggtcg tgctcgcggt gtcgctcggc gcctacaaga agctcaatag
     3781 cgatcccggc ccctgcgacg agctgctgga ggccgacggc aggctgcgtc gcatgagcga
     3841 gcagatcggc ctcgtgccga cacaggcctt gcagctgtgg agccgcgaca gcatggccgc
     3901 gctcggctgg gagggcgcgc cagccactgt cgccggcgcc aagatgctcg atgtctgggc
     3961 ggatatgagc cagaccctcc acgctgagga ctggcgcgac gagcccgccc cgccgctctc
     4021 cgtccattat ctctgcggca cctacaagac gcggctcttc gccgagccgg cttctgccgg
     4081 cgacgtgatg gcgagggcga gcgtcgagct caccgccagc atgggcgatt ggctcgacgc
     4141 tttcggcacg gcgatctggc cgaaggcgac gacgccgggt gctgccttcg attggtgcgt
     4201 gctttgggat tccgccaatg gcgacggccg gcagcgtctg caggcgcagt atatccgcgc
     4261 caatatcgcg cccagcgaat gctgcccggg ctcgtcggct ggctcgctgc cctttcggct
     4321 gcgcagcgac gagacggcct tcgccaacct cgtcctcgcg ggctgctatc tgcgcaccgg
     4381 cctcaactcg acttgcgtcg agggtgcggt gatgtcgggc atgcaggcat cgcgcgcgat
     4441 ctgcggcagc cctgacaaga tcctggccga acatttcctc tgatcgacca accgagcgat
     4501 gaccgaaagc gaggaggcat gaaggacctg ccagcttaca tttccgaccg cggccatggc
     4561 gagatctgct acgaggtgcc gctttcgatc tcgggcgcca cggtctacgc cttcgcgctt
     4621 catgcccggc acgaagcgct ccaggcgctg gtcgacagcc agctcaatgc ggcggcgagc
     4681 ccgacggtgc actacgaagc ggtcggccac agcgtgctac ttatctattt ccacgccgag
     4741 cattccacct cgacgaccga gacgatcggc tggctgcccg accgggaatc gatggtctgg
     4801 gtgctggtgg tgcagaagac cgcgcaaggc agccggctga tgttctggat cccctatctc
     4861 ctcatcgacg ccatgagcgg catggtcacc ggccgcgaag tgtggggcta tcgcaagtcg
     4921 ctcgccacca tcgatctgcc catcgcggca accgatccgg cccagttcgt cgcgcgcacc
     4981 gtcgtgttca agaccttctc gccggacacg caaggcttgg tcgagccgct catcaccgtg
     5041 agccgcaacg gcccgctggg cccgctccag acggcgtggc gggatcccga ggatgtcgtg
     5101 aaaggtgcga tcgccatgct ccgcgagagc ttcagcgaat tcgacatgtc ggactggctg
     5161 aatgctgcga cggtgatcga gggcctcggc aagatcgcgg cgacgctgcg catccccgtc
     5221 atcaacctca agcagttccg cgacgtgggc gacagcgcca aggcctgcta ccaggcgctc
     5281 gtcgaatccc cttgcgagct cgtgacgctc catggcgcgg ggccgctgcc tggcgatttc
     5341 acagcctcct tcgtcacttg cgacagccat cgcgtggttg ccgatctcgg cctcggcacg
     5401 cccgccccgg gcgccacatc ggtgccggtg cgcttcgctt tctggtggaa gatggacttc
     5461 aagaccctcg acggctcggt ggtctggcag gcctcctgag ccgggccgac tgctctcaat
     5521 ccggctgcgc cggcgacggt ttttgcgtga tcgcctcggc ggcgcgctga cccgattcga
     5581 aggcgctctc gaccgagccg ccattgatgc tcgacagcgt ccagtcgccg gcgaggaaga
     5641 cattggcgaa tccgctcttg ccgggatcga ggcgatattg gacgctgccc gggaaggtct
     5701 gcacatactg gtccgaatag ccgatattga ctcgataata gcggtcggtg acgacgctcc
     5761 ggtcgagggc cttgccattg gggcaaactc ccggccagag cacgcccgca ttctcgtcga
     5821 gccaagcctc ggtcctctga accacgctct tcgtctgcgc gtcgatgaag gtcggattct
     5881 cgcccacggg caacggcgcg gcgcaatgat agacgccgca gagatactga caggatccag
     5941 ggtccccggc gccgccctcg cgcacttgcg acatgtccgc ccaggatgac agatcctggg
     6001 cgtaggcggt gagcacgctc tgtccgttct tccagcccag tcccgagaga tcgggcttca
     6061 tccagagctg caccgattgt gtcggcaccg ccgtcatgtt gtcgagcatg ttgcgccacg
     6121 gcacgctcgc cgcgagctgc gccgagatcg gcttgagcgc gagcggcggc atcgccagca
     6181 cgacgacatc gaaatcctgg ccgcggcgca gcgtctgctc gccgaccttg aaggtgcacc
     6241 acgggctctc gagcgccaga ccctttgtcc cgaggacgtc gccatcgacg atctgatccc
     6301 acaagggctc ggccggccac ctgtcgtcac cctcctgcaa cggcaggtag cggccattct
     6361 tcacatcgag ctggcggtcg atctcgatcg tctcgaccag gccgtccttg acgccaaggt
     6421 cacgcacgcg gtggaagaaa ttgatggcga catggcggcc ggcgagatag cggtagagcg
     6481 gcgtgaagat gacgtcgccg gcgcccgcct gcatccgcca aagcggcgcg ttgcgatagc
     6541 cgaagcacat tctcatgacg aggcgaagag cggcgcccgc ggccatcttc gcggcgccgg
     6601 gcttgccggc ctctcccgct tcgtaagcga aagcgagatc gtagatcgcc tggacaggac
     6661 ccggcccgag cgcgtcgtcc ggagcgccat ggctcttcag ccaggcgcga aaatcgaggg
     6721 cgttcagcgc atcgaagccg gcatcgccct tgggcagcac gtcctcgaga tagccgatga
     6781 ggccggcgaa gccgagcgag atcatcacat agaggtgaaa gccccactct tcgcaagagc
     6841 gcagcgtcgc cacgaggaaa gtgttgaagc attcctggag gcgccgcagc tcgcgcagca
     6901 ggtccgaagc gacgctatga tcggccggca gacgttcgcc gagcagccat gccgtctcga
     6961 gagagcattc ggcatagagc ttccggaaga agccggcgat ctcggtcgaa acgccggcga
     7021 agcgttcctc gaaaagcacc gtggtctcgg cgagcgctcc gtcgacatga agcatcgaga
     7081 agaaatcaat cttgatcacc gagccggcga cgcgcctgcc tagaaggtcg tgcagcaagg
     7141 gccagacgct gctccgcagc gcggcaatga ggcatttggg cgcgcgcgcc agatagtcga
     7201 ggccggtatc atcaagatcg ccggggttac cgggtagctc gggaaattcg atcgtccacg
     7261 ggctccaggt cgttccaccc ttgccatccg gaacagcaaa gccgaggctg acgagacgct
     7321 gcggcgtgaa ggcatcggtc cagctccgga agcgatcgtc gggccggcgc gtcaacggat
     7381 agacagcgcg catcgtggcg aaggccgtcc ggtaaaagcc catgaacatg tggagcccgt
     7441 gctcctcgat gcgctcgccc gcgctgtagt tgcgcgcgct tgcgcatttg ccgccgagcc
     7501 gccagccctg cgtatagagc gtgacctcga agcggtcgcg cagctcctgc gaggacgaaa
     7561 gccagaaggc cgcagcaaca ccacccggac ctccgccgag aatggcgacc ttggtcttct
     7621 ccgccgacat gtctccctcc ccgccgcccg cctccgaggc tactacggcg ctcgcatgcg
     7681 gaacagggtg acgcttggcg acgccacgag cggcgcgggc gcggggggag gcacgtccaa
     7741 gtcctggctg gcctttcgca aggggagaca ccccaagctc aaaccgccgt cgatcacaag
     7801 attcttggcc gacccgatcc aaagcccttg gccggtgagg gatcccttgg ctccgcttgg
     7861 ggggcggaat catggcatcc gtggatatcc ctggtaaaag agccagggtc gatggcgaac
     7921 gcccgacgga gttagggcga tccggtttcc gcaggatcga ggcttctgaa tggttgatcc
     7981 tttctgcggt cgcgatcggt gggacagctg agtgagaacc atcgaatcga catggtcgag
     8041 gctacaccta gcgttatcag ggctattttt cgcagccccg tcgaaaatat tccgcaaaag
     8101 cgtcgtatag ggcataattt aactttcctg gctagaccga cttaccgatg ttctccgact
     8161 ctttttcttg gctgcatctc acagatttcc actacggaat gaaggggcag gattgccttt
     8221 ggcctaactt gcgtgagccg tttctcgaga gtctcgcagt gctgcatgaa aagtgtggcc
     8281 cctggcaagc cgtgctgttt actggcgatc tagtgcaatc gggcgagtcc gaccaatata
     8341 aaaaaatgca agacgaagtg ctcgcgcctc tgtggcgtag acttgacgag cttggatcga
     8401 aaaacgtagc gctacttgca gtgcccggca atcatgacct gtatcgtccc aagccagact
     8461 cgccaacttt cgacgacccg gctatggaaa cgctgcttca gccggagggc ttcaatcgca
     8521 tcggagctaa attttggacc aactcctcgg gtgcgtatcg gcgcgctatc agagaggtgt
     8581 tcgccgctta caatgagtgg tggagtaggg ctccggggcg acccccaaac gtgaaggacg
     8641 gcgcattacc cggtgacttc tccgtatcca tcgagcacgg caagcgaaag atcggaattg
     8701 ttgggctaaa caccgcgttt cttcagcttg gtatcgatga ctacgcggga cgcctcacct
     8761 ggaatgctca acagctccac gctgtttgcg acggcggcat cgatctctgg ataaggcagc
     8821 acgatgtgtg cctgcttctt acgcaccatg gacctaattg gctcaccccg caagcgcgag
     8881 aacacggcgc tgcggaaatc gcaccgcctg ggcggtttgc ggctcagttc tttgggcatc
     8941 agcacgaaac gagcatcgaa tacatacgcc gtggcggcgg ccgagcagcg atccgcctct
     9001 gccaaggatg ctccgccttt ggccttgata gatttggcga cccgccgaca accaagcgaa
     9061 cgcatggcta cgcagccggt cgcatcgact tcggacgcgg tatggcgacg ttgcgcctct
     9121 ggccgcgcgt cgcgacaaga gggacaggac catggcgctt catccccgac cacgagcacg
     9181 ccgtacttga ccccgatgag gggacggaag ccgaagaggt cgcagctagc tcgcgagcgc
     9241 cgcgcgcacc gccggcaccg accatatcag gagctgacag cttcattccg cattcgacgt
     9301 tgccagcgcg gcggcccttc tttgggcgga aagccggctt agagaagatc gcgaaatatc
     9361 tgctgccgga tgacagaagc tggggagttg tgctcgatgg tccaggtggc gtgggaaaga
     9421 cgtcgctggc gatagaagca gcccacggtg caccagctga gcatttcccg ctgaagctat
     9481 ggatcactgc taagagccgc gagctacatc ctgaaggcga atcaagatta acagatcatc
     9541 gtgtcgacga ctattacggg ttgctcaacg aactcggact ttcactcggt cgagaagaca
     9601 ttccgcgtgc cccgcccgag gagaggcccg cactcatccg gcacgcgcta actcaacaca
     9661 aggcgttact cgttgtagat aacctagagt cttttcatac ggaggatcgc cgtcgtctat
     9721 ttgaattgat gggtagcctc cccacgacct gccgggccat cgtcactagt cgccgccgta
     9781 ctgatggttc cctggcggct catatgatcc g
//
LOCUS       DASYNS010000007         4036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3123062, whole genome shotgun sequence.
ACCESSION   DASYNS010000007 DASYNS010000000
VERSION     DASYNS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3123062"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..717
                     /locus_tag="VGP50_00240"
     CDS             223..717
                     /locus_tag="VGP50_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEV8025819.1"
                     /translation="MNAFRLAPFLVLVASVALVGGALLFQYVGHLAPCELCLYQRWPY
                     YAAIPLSLVAVLAGRRPATLGLLALCALLFIVGGGLAFYHVGVEQHWFAGPTVCTDSL
                     STSGSVEDFTARLLATQPVRCDEIAWSLFGVSLAGWNFLASLGLVVFCGATLRALARS
                     PRFA"
     gene            714..1262
                     /locus_tag="VGP50_00245"
     CDS             714..1262
                     /locus_tag="VGP50_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEV8025820.1"
                     /translation="MTAPSPRLPGDPPRAEGIARMIRVDHAGEYGAARIYDGQLAVLG
                     RSPSMPVIRRMAEQEKRHLAAFDRLVVERRVRPTLLSPLWHVAGFALGAATALLGERA
                     AMACTVAVEDVIDAHYAQQIATLGAEEPELKATLTEFRGDEIEHRDTGLAHGAAEAPA
                     YALLSTAIKTGSRLAIWLSTRI"
     gene            1286..2113
                     /locus_tag="VGP50_00250"
     CDS             1286..2113
                     /locus_tag="VGP50_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8025821.1"
                     /translation="MKAQGKTILITGATDGVGRRVALDLGEAGARLLIHGRDRDRAEV
                     LIDELARHGNRDVRFYRADLASLAEVRGLAEAVRRDEKRLDVVISNAGIGTAVGGRER
                     SLSADGHELRFAVNYLAGFLLVRLLLPLLRAATPSRVVNVASAGQTPIDFDDVMLERD
                     YTGGRAYMQSKLAQIMFTFDLARELEDSGVTANALHPATYMNTTMVRQSGATPISTVE
                     EGAAAICRLAVSPEVEGKTGLYFDRMQATRANAQAYDERARDRLRRLSYELTGLKPE"
     gene            2208..2678
                     /locus_tag="VGP50_00255"
     CDS             2208..2678
                     /locus_tag="VGP50_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025822.1"
                     /translation="MEVALATWDTDSDEAGPLLCPSAQPDEDAVVIAIVGKRNASEPV
                     RMLPRPIPLDAVGHLVPESMPVTDMLRLAAPCAERRCGHFDDGRCMLASRVARLPELM
                     DAFSPCSFRPICRWWRQEGAAVCRRCPQITDDPFRVSDLLRRVAEPAPAPSEAA"
     gene            complement(2948..3562)
                     /locus_tag="VGP50_00260"
     CDS             complement(2948..3562)
                     /locus_tag="VGP50_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025823.1"
                     /translation="MAAVAFLALTLPTMVRAETPSAGREALEKLLNAAPEQDHPTPDE
                     EMKLFGAIYARPPTDNDIIIPWLRRRMDHLQSVFAFELSRRLFPDDRGAALEWFVIGE
                     TRFVYDLARCTDSAEGSDVVLATIERSFRPLPEYLWDHPDEHAAALRRAAARHDLFAP
                     ETSPTCSDATTPLDQWPSVEERLRARMVKEAEDLEAKRGAPPSR"
     gene            complement(3602..>4036)
                     /locus_tag="VGP50_00265"
     CDS             complement(3602..>4036)
                     /locus_tag="VGP50_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEV8025824.1"
                     /translation="TEVSARLGRRLKFLVGKPGLDGHSNGAEQIALKARDAGFEVVYE
                     GIRLTPAQIVNAALEEGVHVVGVSILSGSHVPLVSEILSGLKAHGLGHIPVVAGGIIP
                     PTDEAALKRAGVARVYTPKDYDLTAIISDIVELVANEPRLAA"
BASE COUNT          597 a         1361 c         1374 g          704 t
ORIGIN      
        1 ggacagcgat cccgagctgc gcaatgtcat gcaccgcttc gatttcgtga tcgtcgtggt
       61 cctcgtcctc ggcatcgcct ggtatgtccg gcggcattgg cagcatcggc tgtcgaagga
      121 gtaagcttgc gtatgagaag agagggccat acccccctcc ctatccctcc ccctcaaggg
      181 gggaggggaa gtccaattgc ggtctttccc ggcggcgtcc gtatgaatgc attccgtctc
      241 gcccctttcc tcgtccttgt cgccagcgtg gcgctggtgg gaggcgcgct gctgtttcag
      301 tatgtcggcc acctggcgcc gtgcgagctc tgcctctatc agcgctggcc ctattatgcc
      361 gcgatcccgt tgtcgctcgt ggcggtgctc gcgggacggc ggcccgcgac gctcgggctg
      421 ctcgcgctct gtgccctgct gttcatcgtc ggcggaggtc tagcctttta ccatgtcggc
      481 gtcgagcagc attggttcgc cggcccgaca gtctgcaccg acagcctctc cacgagcggg
      541 agcgtcgagg acttcacggc gcggctgctg gcaacgcaac cggtgcgctg cgacgagatc
      601 gcgtggtcgc tctttggcgt ctcgctcgcc ggctggaact tcctggcgtc gctcgggctc
      661 gtcgtcttct gcggtgccac gctgcgggcg ctcgcgcgat cgccgaggtt cgcatgaccg
      721 cgccgtcgcc gcgcctgccc ggggacccgc cgcgcgcgga agggatcgcg cgcatgatcc
      781 gggtcgatca cgcgggcgag tatggcgcgg cgcgcatcta tgacggacag ctcgccgtgc
      841 tgggacgctc gccgtccatg ccggtgatcc gccgcatggc cgagcaggag aagcgccacc
      901 tcgccgcctt cgaccggctg gtcgtcgagc gtcgcgtgcg gccgaccttg ctgtcgccgc
      961 tctggcatgt ggcgggcttc gccctgggcg ccgcgacggc gctcctgggc gagcgggccg
     1021 ccatggcctg caccgttgcc gtcgaggacg tgatcgacgc gcattatgcg caacagatcg
     1081 cgacgctggg tgccgaggag cccgagctca aagcgacgct caccgagttt cgcggcgacg
     1141 agatcgagca ccgcgacacg ggccttgccc atggcgccgc cgaggcgccg gcctatgcgc
     1201 tgctgagcac cgccatcaag acgggctcgc gccttgccat ctggctctcg acgcggattt
     1261 gacgacaacg cggaggaaca ggaccatgaa agcacaaggc aagacgatcc tgatcaccgg
     1321 cgcaacggac ggtgtcgggc ggcgcgtggc gctcgatctc ggcgaggcgg gcgcccgcct
     1381 cctgatccat ggccgcgacc gcgatcgcgc cgaggtgctg atcgacgagc tcgcccggca
     1441 cggcaatcgc gacgtgcggt tctatcgcgc cgatcttgcc tcgctcgccg aggtgcgcgg
     1501 cctcgccgag gcggtgcggc gcgacgagaa gcgcctcgac gtggtgatca gcaatgcagg
     1561 catcggcacg gcggtcggcg ggcgcgagcg cagcctcagc gccgacggcc atgagctgcg
     1621 ctttgccgtc aactatctcg cgggcttcct gctggtgcgg ctgctgctgc cgctgctccg
     1681 cgcggcgacg ccgtcgcgcg tcgtcaatgt cgcctccgcc ggccagacgc cgatcgattt
     1741 cgacgacgtc atgctggaac gagactacac cggcggcagg gcctatatgc agagcaagct
     1801 cgcgcagatc atgttcacct tcgatctcgc gcgcgagctc gaagacagcg gcgtcaccgc
     1861 caacgcgctt catcccgcca cctacatgaa cacgaccatg gtgcgccaga gcggcgccac
     1921 gcccatcagc acggtggaag agggcgcggc ggcgatctgc cgcctcgccg tctcgccgga
     1981 agtggaaggc aagaccggcc tctatttcga ccgcatgcaa gcgacgcgcg ccaacgcgca
     2041 agcctatgac gagcgcgcgc gggaccggct gcgccgcctg agctatgagc tcacggggtt
     2101 gaagccggag tgaccgcgga gagatagggc actcgactct tccatcttgc ttcgcctgtg
     2161 atttgcgtca caggcgagcg cgctccgttc cgtgtagaag cgcaggcatg gaagtcgcgc
     2221 tagccacatg ggacaccgac agcgatgagg cgggcccgct gctctgtccc agcgcccagc
     2281 cggacgagga cgccgtcgtc attgccatcg tcggcaagcg gaacgcttcg gaacccgtca
     2341 ggatgctgcc gcggccgatt cctctcgatg ccgtcggcca tctcgttccc gagtccatgc
     2401 cggtcaccga catgctgcgc ctcgctgcgc cttgcgcgga gcggcgctgc ggccatttcg
     2461 acgacgggcg ctgcatgctc gcgtcgcgcg tcgcgcggtt gcccgagctc atggatgcgt
     2521 tctcgccgtg ctcgtttcga cccatctgtc gctggtggcg tcaggagggc gcagccgtct
     2581 gccggcgctg cccccagatc acggacgacc cgttccgggt gagcgacctg ctccgccggg
     2641 tcgccgagcc ggcgcccgcg ccgtccgagg cagcttgaac ctgattcatt ttcttgtgcg
     2701 ccgccaggcg cgctctcgtc ggagattgag cgcctcggtc gatcgattgg aggcacctga
     2761 acgcccgttg gcgaacgcag aggacgcaga ggttccgcgg aaggtgcaga ggatgacttt
     2821 ttgtttctgc gtcctctgcg gagcctcggc gtcctccgcg ttcatctttc tttattgtgc
     2881 cgctgccgaa ctcaggaacg tcgatcgatc ggaggggtag agcgggtcta cgtgaaccaa
     2941 gatgcgtcta ccgcgacggc ggcgctccac gctttgcctc caggtcctcg gcctccttca
     3001 ccatccgggc gcggagcctc tcctcgacgg acggccactg gtcgagcggc gttgtggcat
     3061 cgctgcaggt cggcgatgtc tccggtgcaa agaggtcgtg cctcgcggcg gcgcggcgaa
     3121 gcgcggcggc atgctcgtcc gggtgatccc agaggtactc gggaagcggg cggaaggagc
     3181 gctcgatcgt cgccaagacg acatcgctac cctccgccga atcggtgcac cgggcgaggt
     3241 cgtaaacgaa gcgcgtctct ccgatcacaa accattccaa ggccgcgcct cgatcgtcgg
     3301 gaaacagccg ccgtgacagc tcgaaggcga atacggactg cagatggtcc atgcggcggc
     3361 gcagccatgg gatgatgatg tcattgtccg tcggcggccg cgcgtagatg gcgccgaaca
     3421 gcttcatctc ctcgtccggt gtcggatgat cttgttccgg cgcagcgttc agcagcttct
     3481 ccagggcctc gcgtccggcg gagggcgtct cggctcgaac catggtcgga agcgtgagcg
     3541 cgaggaacgc gacagcggcc accgatctca gtcgacgcaa aaggttctcc gccggtcgaa
     3601 cttacgccgc aagccggggt tcgttcgcga ccagctcgac gatgtcgctg atgatggcgg
     3661 tgaggtcgta gtccttgggc gtgtagacgc gggccacgcc ggcacgcttg agtgccgctt
     3721 cgtccgtggg cgggatgatg ccgccggcca ccaccgggat gtgcccgagg ccgtgcgctt
     3781 tgaggccgct caggatctcg ctcaccagcg gcacgtggct gccggagagg atcgagacgc
     3841 ccaccacatg cacgccttcc tcgagcgcgg cgttgacgat ctgcgcgggg gtgaggcgga
     3901 tgccttcata gacgacctcg aagccggcgt cgcgcgcctt gagcgcgatc tgctcggcgc
     3961 cgttcgaatg gccgtcgagg ccgggcttgc cgaccaggaa tttcaatcgc cggccgagcc
     4021 gcgccgagac ctcggt
//
LOCUS       DASYNS010000008         7550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2498750, whole genome shotgun sequence.
ACCESSION   DASYNS010000008 DASYNS010000000
VERSION     DASYNS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7550
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2498750"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VGP50_00270"
     CDS             complement(<1..793)
                     /locus_tag="VGP50_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025825.1"
                     /translation="MRIHQSGDSATPAFGRFAGLFLALFVAAAIPVLSCDSLPLFDYP
                     NHLARMHILRDLAHSPTLQQFYEVAWRPLPNLAMDAIVPVLGRVMPLAWAGKVFVLLT
                     LFLLACGPATLHRVLFGAWSAWSCVAFLLLYSRTLLWGFLNYLFGLGLALLALALWLA
                     LARRPAALRLVAGVFLALALFFAHLLAFGLYGVMVMGYEAGRILRGRASIQQGLAEIV
                     IAGLGFVPALGVLVLGSPASMSGAVRWGHLARKIDLLFSVFDNYDR"
     gene            917..2029
                     /gene="lptF"
                     /locus_tag="VGP50_00275"
     CDS             917..2029
                     /gene="lptF"
                     /locus_tag="VGP50_00275"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEV8025826.1"
                     /translation="MRGITRYVLRQTLTNMAFIAAGFTAAVWLVQSARLIDLIVNRGL
                     STTLFAYLAVLTLPRFVVVVLPIAVFIAVLFSYNRLLAESELVVMRAAGMSQSELARP
                     ALIAGAIAMLILTSMSVYFLPAANRAFKDLQFEIRNRFASALLQDGVFNTLSESLTIY
                     MRSRDTNGELVGLLIHDTRDADKPITILAERGAFADIDGGPRLLMVNGSRQQMDRTTG
                     KLSVLSFEKYTLDLASFRDAPGVRDRQPDERYLQELLFPADPSRVGDAAFLIEANFRL
                     ITPLTALALAVIPVACLLTGEFNRRGQTRRVLVAVLIAFAFEALDIGLKNLAGRTAVA
                     VLFLYLNVMAPICATLWLLWRDGRHPTLRRVLSAAG"
     gene            2037..3134
                     /gene="lptG"
                     /locus_tag="VGP50_00280"
     CDS             2037..3134
                     /gene="lptG"
                     /locus_tag="VGP50_00280"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEV8025827.1"
                     /translation="MSPWKTLFRYILAKFFVWCASVFLAMEAIVFIFDYIELLKRGGT
                     RPEATFLALLEMAMLKQPDMAQKMMPFAILFGTMMAFWRLTRNNELVVARAAGVSVWQ
                     FLTPPLAGAAFVGIVAVTVFNPIASILQVRYETLESRILRGSGDQLALSPSGLWLRQS
                     DDEGHHSVIHAARLLPRRTELEDVMVLSFTNDTQLTSRLDAKSAVLESGRWEVRDGTR
                     WTTGLPSAPFQRAEVPTKLTARKLQESFAAPETMSFWELPGFIKLLESSGFNAQHHRL
                     YFDTLLARPFLFTAMVLVAAAFSLRMQRRGGTGLMIAGGAGTGLLLYSLSYVVFALGQ
                     SAAVPVLLAAWTPAGVSLLLGASMLLHLEDG"
     gene            3377..5584
                     /gene="lptD"
                     /locus_tag="VGP50_00285"
     CDS             3377..5584
                     /gene="lptD"
                     /locus_tag="VGP50_00285"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEV8025828.1"
                     /translation="MARGVIHLGAFLAAVSLPAAAAEPAHTGSPEPALFSADEVQYDE
                     DLGLVVAKGNVEISQNDQTVLADTVTYNQRTDTITASGHVSLLQPGGDVVFADFVELT
                     NNMQEGFLRDIRVLMLDRSRLAGNTGRRINGNRIELRRGVYTACEPCAKDPNAPPLWQ
                     IKAEEIISDKELLIIEYRDALMEIDGIPVFYTPYLSQPDPSVKRRSGFLGPSAGNSPS
                     NGLHISIPYYWDIDKDKDTTFMPEFTTQGGTFLGDEYRQRFSNGQLVATGSITVGSKA
                     STLVDTEPVSGVRGHLFADGELDLNEDWRTGLDVQRVSDQTYLLRYQITAPPDFLASH
                     VYAERFGENSYANISSWAFQSLQPGVGDSIQPIVAPVADYQWLSDPDVLGGQVAVEGN
                     ALDLLRKTGIDTRRLSLGETWRAPVTGPFGERYTFSLGLRTDGYQSDNIPIANEGNAT
                     QNAFAGRALPQAAITMQYPWIRRADGFSEVIEPVVMVAASPYGGNPGTIPNEDSQGLE
                     FDETSLFLPNRFPGFDRVDSGQRVDYGFRAGVYGDSGGSTRFIVGQSYAMQVNDNFEA
                     GSGLEHHLSDVVGRVTLSPGPYLDLIYRFRLDHEDLALRRQEAVISTGPAALRLSLSY
                     LQIAAIPNVPELPKEKQIVGSLSAQLTRFWSVQLQATRDLAPSVPVTATGTPINTVGT
                     TETLYSGAAVTYRDECLALVASVTQSGIANGDVRPGTSFLFTIVFKNLGDVAL"
     gene            5724..6971
                     /locus_tag="VGP50_00290"
     CDS             5724..6971
                     /locus_tag="VGP50_00290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV8025829.1"
                     /translation="MKQLLILAFLLLLAGGASAQETRIAAIVNDDVVSLDDLNNRMRL
                     VMRSSGLEDTPENRERMSGQVLRSLIDEKLQMQEVKRLSVNVPKEEVDEAFHRIEQNN
                     NMPKGALDQFLSDAGIPRTSLTDQITASLGWGKLVRGRLLQEVTISEEEVNEVVNQLQ
                     QNADVPQDRVSEIFIAIDNPNQADEAKRTADRLIEQITAGAKFGAVAQQFSQSPSAAQ
                     GGDIGWVTPNQLLPEIGKAIEKMKPGQMSYPVRTTAGYYVLYLVDSRKLGQPDPNEAQ
                     LSLVQVTLPLVQTASSEEQQQVLLQAQQISSSAKSCGELAKFGRERAPQSSREIPAIK
                     AGDLPLQMRQVVLGLGIAEASKPMPAPGGIAIVMVCERKDPPGGLPSREDITEQLGRV
                     RLDALARRYLRDLRRSAYVDIRG"
     gene            6968..>7550
                     /locus_tag="VGP50_00295"
     CDS             6968..>7550
                     /locus_tag="VGP50_00295"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEV8025830.1"
                     /translation="MTLPLALTMGEPAGIGGELTLLAWLKRGKEGLPPFFALDDPARL
                     ERLAASLGWTVPLRVIAEPAEAVAVFPEALPVLPVALATRVTPGRPDASNAPAVLRAI
                     TTAVSLVKARRAAALVTNPIQKKLLYSAGFRHAGHTEFLAELAGGAAPAMMLVCPGLR
                     VVPVTIHLALRDAVAALSCEKIVAAGRITAAGLV"
BASE COUNT         1294 a         2608 c         2388 g         1260 t
ORIGIN      
        1 cacggtcata gttgtcgaag acgctgaaca ggagatcgat cttgcgcgcg agatggcccc
       61 atcgcacggc acccgacatg ctcgcggggc tgccgaggac caggaccccg agcgccggga
      121 cgaaacccag gccggcgatc acgatttcgg cgaggccctg ctggatcgac gcccggccgc
      181 gcaggatccg ccccgcctcg tagcccatca ccatcacgcc gtagaggccg aaggcgagaa
      241 gatgggcgaa gaacagcgcc agtgccagaa aaacgccggc aaccaggcgc agcgccgccg
      301 gccgccgcgc cagcgccagc cacaaggcga gcgccaggag agcgaggccg aggccgaaga
      361 ggtagttcaa aaaaccccag agcaaggtgc ggctgtagag cagcaggaag gcgacgcagg
      421 accacgccga ccaggcgccg aagagcacgc gatggagggt cgcaggccca caagccaaca
      481 ggaacagggt cagcagcacg aagaccttgc cggcccaggc gagcggcatg acgcggccaa
      541 gaaccggcac gatcgcgtcc atcgccagat tggggagagg tcgccaggca acctcataga
      601 attgctgaag cgtcggcgag tgggcgagat cgcgcaggat atgcatgcgt gccaaatggt
      661 tcgggtaatc gaacagcggc agactgtcgc agctgaggac cgggatcgcc gccgcgacga
      721 agagcgccag gaaaagtccg gcaaatcgcc cgaaggccgg cgtcgcagaa tcgccactct
      781 gatgaatccg caccggcgct cccttttgcc cgatcggccc cgcggagagt tccacgcaac
      841 gcacgccgcc gcaagcggat gatcccacgc aaacttcgct ctaggctcgg cggcgaaggt
      901 ccctataatc ggccgcatga gggggatcac gcgctacgtc ctgcggcaga cgctcacgaa
      961 catggctttc atcgcggccg gcttcacggc ggccgtgtgg ctcgtccagt cggcgcggct
     1021 catcgacctc atcgtgaacc gcggcctctc gaccaccctc ttcgcctatc tcgccgtgct
     1081 cacgctgccc cgcttcgtcg tcgtggtgct gcccatcgcg gtcttcattg ccgtgctgtt
     1141 ctcgtataac cggctgctcg ccgagagcga gctcgtcgta atgcgggctg ccggcatgag
     1201 ccagtcggag ctggcccgcc cggcgctgat cgccggcgcc atcgccatgc tgattctgac
     1261 atcgatgtcg gtctattttc tgccggcggc caaccgcgct ttcaaggacc tgcaattcga
     1321 gatccgcaac cgcttcgcct cggcgctgct ccaggacggc gtcttcaaca cgctctcgga
     1381 aagcctgacc atctacatgc gcagccgcga caccaatggc gagctggtcg gccttttgat
     1441 ccacgacacg cgcgatgccg acaagccgat caccattctc gcggagcgtg gcgcctttgc
     1501 cgatatcgac ggcgggccgc gcctcctcat ggtcaatggc agccgccagc agatggaccg
     1561 caccaccggc aagctctcgg tgttgagctt cgagaagtat acgctcgacc tcgcgagctt
     1621 ccgcgacgcg cccggcgtgc gcgaccgtca gcccgacgaa cgctacctgc aagagttgct
     1681 ttttccggcc gatcccagcc gcgtcggcga cgccgcgttt ctcatcgaag cgaatttccg
     1741 cttgatcacg ccgctcacgg cgctcgcgct cgcggtcatc ccggtcgcct gcctgctcac
     1801 cggcgagttc aaccgtcgcg gccagacgcg ccgcgtcctg gtggcggtgc tcatcgcctt
     1861 tgccttcgag gcgctcgata tcgggctcaa aaatctggcg gggcggacgg cggtcgcggt
     1921 gctgttcctc tacctcaacg tcatggcgcc gatctgcgcc acgctttggc tgttgtggcg
     1981 cgacggccgc catccgacgc tgcgccgcgt cttgtcggcg gctggctgag cgccccatgt
     2041 cgccctggaa gaccctgttc cgctacatcc tcgccaagtt cttcgtctgg tgcgcctccg
     2101 tcttcctggc gatggaggcg atcgtcttca tcttcgacta tatcgagctg ttgaagcgcg
     2161 gcggcacgcg gcccgaggcg accttcctgg cattgctcga gatggcgatg ttgaagcagc
     2221 cggacatggc gcagaaaatg atgccgttcg ccatcctgtt cggcacgatg atggcattct
     2281 ggcgcctgac gcgaaacaac gagctcgtcg tcgcgcgcgc cgccggcgtg tcggtctggc
     2341 agttcctgac gccgccgctc gcgggtgcgg ccttcgtcgg catcgtcgcg gtgaccgtgt
     2401 tcaacccgat cgcctcgatt ctgcaggtgc gttacgagac cttggaaagc cgcatcctgc
     2461 gcggcagcgg cgaccagctc gccttgtcgc cctcgggcct ttggctgcgg cagagcgacg
     2521 atgagggcca ccactcggtg atccatgccg cgcgcctcct gccgcgccgg accgaactcg
     2581 aggacgtgat ggtgctctcg ttcaccaacg acacgcagct caccagtcgg ctcgacgcca
     2641 agtcggcggt gctcgagtcc ggcagatggg aggtcaggga cggcacgcgc tggacgaccg
     2701 gcctgccctc ggcgcccttc cagcgcgccg aggtgccgac caagctgacg gcgcgcaagc
     2761 tccaggagag cttcgccgcg cccgagacga tgtccttctg ggaactgccg ggcttcatca
     2821 agctcctcga aagctcgggc ttcaacgcgc agcatcatcg cctctatttc gatacgctcc
     2881 tcgcgcgacc tttcctcttc accgccatgg tgctggtcgc cgccgccttc agcctgcgca
     2941 tgcagcgccg cggcggcacc ggcctgatga tcgccggcgg cgccggcacc gggctcctcc
     3001 tgtattcgct ctcctatgtg gtattcgcgc tgggccagtc ggcggccgtt ccagtcctgc
     3061 tggccgcgtg gacgccggcc ggcgtcagtc tgctcctcgg cgcaagcatg ctgctgcatt
     3121 tggaagacgg ttagaacatg tcgatttgag cgggctctgc cctaagcaga gttgcacaga
     3181 tcgcgatacg gacgcacaaa tcccctctcc cttcggggag agggtcaggg tgagggggcc
     3241 gcgccacgat gtcattcagc gttgacggtg tgtttttgca cgcgtcatct cacggggtcg
     3301 ccccctcacc ccggccctct ccccggaggg gagaggggga tgtggggtgc ggtcaaagga
     3361 acgccgctta gacatcatgg cgcggggggt catccatctc ggcgcgtttt tggccgccgt
     3421 gtcgcttccg gccgcggcgg ccgagccggc gcataccggc agcccggagc cggcgctctt
     3481 cagcgccgac gaggtgcaat atgacgagga tctcggactc gtcgtcgcca agggcaacgt
     3541 cgagatcagc cagaacgatc agaccgtgct ggccgatact gtcacctaca accagcgcac
     3601 cgacacgatc accgcctccg gccatgtgag tttgctgcag ccgggcggcg acgtcgtgtt
     3661 cgccgatttc gtcgagctca ccaacaacat gcaggagggc ttcctccgcg acatacgcgt
     3721 gctcatgctc gaccgctcgc gcctcgccgg caataccggg cggcgcatca acggcaaccg
     3781 catcgagctg cgccgcggcg tctataccgc ctgcgaaccc tgcgcgaagg atccgaacgc
     3841 gccgccgctg tggcagatca aggccgagga gatcatcagc gacaaggagc tgctgatcat
     3901 cgaataccgc gatgccctga tggagatcga cggtattccc gtcttttata cgccctatct
     3961 gtcgcagccc gatccttcgg tgaagcgccg cagcggcttc ctcgggccga gcgccggtaa
     4021 ttcgccctcg aacggcctcc atatcagcat tccttattac tgggatatcg ataaggacaa
     4081 agacacgacg ttcatgcccg agttcacgac ccagggcggc acgtttctcg gcgacgaata
     4141 tcgccagcgc ttcagcaacg ggcagctcgt ggccaccggg agcatcaccg tcggcagcaa
     4201 agcctcgaca ctggtcgaca ccgagccggt gagcggcgtg cgcggccatc tcttcgccga
     4261 tggcgagctc gatctcaacg aggattggcg caccggcctc gacgtacaac gtgtctccga
     4321 ccagacctat cttctgcgct atcagatcac cgcgccgccg gactttctgg cgagccatgt
     4381 ctatgccgag cgcttcggcg aaaacagcta cgccaatatc agcagctggg cgttccagag
     4441 cctgcagccc ggggtcggcg acagcataca gccgatcgtc gctccggtcg cggactacca
     4501 gtggctgagc gaccccgacg tgctcggcgg tcaagtcgcc gtcgagggca atgccctcga
     4561 tctcctgcgg aaaaccggca tcgacaccag gcggctgtcg ctcggcgaga catggcgcgc
     4621 ccctgtcacc ggaccgtttg gcgagcgata caccttctcc ttgggcctcc ggactgacgg
     4681 ctaccagtcc gacaacatac cgatcgcgaa cgaaggcaac gccacccaga atgcgttcgc
     4741 cggacgcgcc ttgccgcagg ccgccataac gatgcagtac ccctggattc gccgcgccga
     4801 cgggttcagc gaggtgatcg agccggtcgt catggtcgcc gcctcgccct atggcggcaa
     4861 tcccggcacc atcccgaatg aggacagcca gggcctcgaa ttcgacgaga ccagcctctt
     4921 cctgcccaac cgctttccgg gcttcgatcg cgtcgatagc ggccagcgcg tcgattacgg
     4981 cttccgcgcc ggcgtctacg gcgatagcgg cgggagcacg cggttcatcg tcggccagag
     5041 ctacgcgatg caggtgaacg acaacttcga ggccggttcg ggccttgagc accatctctc
     5101 cgacgtggtg ggacgcgtga ccctttcccc tggtccctat ctcgacttga tctaccgctt
     5161 ccggctcgac cacgaagatc tggcgctgcg ccgccaggag gccgtgatct ccaccggtcc
     5221 cgcggccctg cgcctctccc tcagctatct ccagatcgcg gccatcccga atgtgccgga
     5281 gctgccgaag gagaagcaga tcgtcggttc cctctcagcc caactgaccc gcttctggtc
     5341 ggtgcaattg caagcgacgc gcgacctggc gccctccgtg ccggtcacgg cgaccggcac
     5401 gcccatcaat accgtcggca cgacggagac gctctactcg ggggccgcgg tcacctatcg
     5461 cgacgagtgc ctggcgctcg tggcttcggt aacccagtcg ggcatcgcca atggcgacgt
     5521 gcgtccgggc acgtccttct tgtttaccat cgtcttcaag aacctcggcg atgtagccct
     5581 atgagggtgc cggtagcgcg ggcaccccga taagggccga cccgccgccc gagcaatctc
     5641 tgggacgcga catcgcaacg gcggatgtgt attgtgtcat cctcggagca gccgttccgc
     5701 atcctcaagc tttgcccgtg ctcatgaaac agctccttat cctcgctttc ctcctccttc
     5761 tcgccggcgg cgcgagcgcc caggagacgc gcatcgccgc catcgtcaac gacgatgtgg
     5821 tctcgctcga cgatctcaac aatcgcatgc gcctcgtgat gcgctcgtcc ggtctcgagg
     5881 acacgccgga gaaccgcgag cgcatgagcg gccaggtgct gcgctcgctg atcgacgaga
     5941 agttgcagat gcaagaggtc aagcggctca gcgtcaacgt gccgaaagag gaagtcgacg
     6001 aggccttcca ccgcatcgag cagaacaaca acatgcccaa gggcgcgctt gaccagttcc
     6061 tgagcgacgc cggcatcccg aggacctcgc tgaccgacca gatcaccgcc tcgctcggct
     6121 ggggcaagct ggtgcgcggc cggcttctgc aggaggtgac gatttccgag gaggaggtca
     6181 acgaggtcgt caatcagctt cagcaaaatg ccgacgtgcc ccaggatcgc gtttccgaga
     6241 tattcatcgc gatcgacaat ccgaaccagg cggacgaggc caagcggacg gcggacaggc
     6301 tgatcgagca gatcaccgcc ggcgccaaat tcggcgcggt cgcccagcaa ttctcgcaat
     6361 caccgagcgc ggcgcagggc ggcgacatcg gctgggtcac acccaaccag ctgctcccgg
     6421 aaatcggcaa agcgatcgag aagatgaagc ccgggcagat gtcctacccc gtccggacca
     6481 ccgccggata ttacgttctc tatctcgtcg acagccgcaa gctcggtcag cccgatccca
     6541 acgaggcgca gctctcgctc gtccaggtca cgcttccctt ggtgcagacc gcgtcgagcg
     6601 aggagcagca gcaggtcctg ctccaggcgc agcagatcag cagcagcgcc aagagctgcg
     6661 gcgagctcgc caagtttggc cgcgagcgcg cgccgcagtc ctcccgcgag atcccggcga
     6721 tcaaggccgg cgacctgccg ctgcaaatgc gccaggtggt gctcggcctc ggcatcgccg
     6781 aagcgagcaa gccgatgccg gcgccgggcg gcatcgccat cgtcatggtg tgcgagcgca
     6841 aggacccgcc cggcggcttg ccgtcgcgcg aggacatcac ggagcagctc ggccgggtgc
     6901 ggctcgatgc gctcgcgcgt cgctatcttc gcgatctccg gcggagcgcc tatgtggaca
     6961 ttcgtggatg acgctgccgc tggcgctcac gatgggcgag ccggcgggaa tcggcggcga
     7021 gctgacgctg ctcgcctggc tgaagcgtgg caaggagggc ttgccgccgt tcttcgcgct
     7081 cgatgatccg gcgcggctcg aacggctggc cgcgtcgctc ggctggaccg tcccgctgcg
     7141 cgtcatcgcc gagccggccg aggcggtggc ggtctttccc gaggctttgc ccgtcctgcc
     7201 ggtcgcgctc gcgacgcgcg tcaccccggg ccggcccgac gccagcaacg cgcccgcggt
     7261 gctccgtgcc atcacgacgg ccgtgagcct ggtcaaggct cgccgcgcgg cggcgctggt
     7321 gaccaacccg atccagaaga aactactcta ttccgccggt ttccggcatg ccggccacac
     7381 cgagttcctg gccgagctgg cggggggcgc ggcacccgcg atgatgctgg tctgtccggg
     7441 cctgcgcgtg gtgccggtga ccatccactt ggccctgcgc gacgcggtgg cggcgctcag
     7501 ctgcgagaag atcgtcgcgg ccggccggat cacggccgct ggcctcgtgc
//
LOCUS       DASYNS010000009         8753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_627029, whole genome shotgun sequence.
ACCESSION   DASYNS010000009 DASYNS010000000
VERSION     DASYNS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627029"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VGP50_00300"
     CDS             <1..249
                     /locus_tag="VGP50_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV8025831.1"
                     /translation="TGRGAVLESWRSILQNPEAPHVMCHDDTAFLHGDVAIVLCEEEL
                     ADGSLSATNIFVKEDGEWRLVHHQASQLLARGSPAAPA"
     gene            complement(280..1509)
                     /locus_tag="VGP50_00305"
     CDS             complement(280..1509)
                     /locus_tag="VGP50_00305"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV8025832.1"
                     /translation="MSRANALDSGRALAGAALLAPSLAVFAPHGLAPLLGLVALVVLV
                     MDGRRHLETLPGMLWLVALLAALSLWCLASALWSILPEHSLVVALRFALISVAGLLVF
                     GAATRLEPIGRDSVRRAATAGVVIAAVLILVERFSGAALTRMVVTPPVGIELPLSRFD
                     RGAVVMVLFLWPAMAAQRRIGTALAVAAAVVVAVMAMVSAAAKLALLVSIAVALIAWH
                     VPRVVAAALGGCLILFAILLPLAVPDAATAVAMHERAPWIKSSGIHRLLIWRFTADRI
                     AERPILGWGVDASRALPGGHFNFGQALPEAGLGPDAEALPLHPHNAVLQWEVELGIPG
                     ALLALATIIAALWRLGRQKHLSPRERAGALAWAAAALVFAMLSFGAWQEWWLAALWLT
                     GAAYAASITGPPSPVAR"
     gene            complement(1513..1926)
                     /gene="ndk"
                     /locus_tag="VGP50_00310"
     CDS             complement(1513..1926)
                     /gene="ndk"
                     /locus_tag="VGP50_00310"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV8025833.1"
                     /translation="MAVERTLSIIKPDATRRNLTDKINARFKQAGLRIVADKRLTLSR
                     EQAEKFYAVHKSRPFFNDLVKFMISGPVVAQVFEGENAVALNREVMGATDPAKAAPGT
                     IRKDFAESIEANSVHGSDSLETARGEIAFFFPDLK"
     gene            complement(1990..2457)
                     /locus_tag="VGP50_00315"
     CDS             complement(1990..2457)
                     /locus_tag="VGP50_00315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEV8025834.1"
                     /translation="MTEVGFYHLRSTPLERALPRILERALTEGHRVVVMAGSAERVDH
                     LDALLWTYDDASFLPHGTARDGSGDRQPVWLTTADENPNGATMLVLVDGACSARLDAY
                     ARCFDMFDGNDEAAVTAARERWREAKAAGHRLVYWEQSEGKWEKRGAGTAEAG"
     gene            complement(2454..3953)
                     /locus_tag="VGP50_00320"
     CDS             complement(2454..3953)
                     /locus_tag="VGP50_00320"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEV8025835.1"
                     /translation="MKIAFAEPREPNSGTIVVGVLEDRKLTPRAAALDKESGGALKRA
                     MAASRFTGRKDEILGVLAPAKLEVGRVLLIGLGKAQALDAGAMQSLGGQIVAQLNSAG
                     EKEAAVDLDDIPGMNFKPGEAAANLAYGARLRAYRFDKYRTKEKPEQKPSLRELKVMT
                     DHVAEAKRGFTPLDKVADGVFLTRDLVSEPANIIYPETLAEAARTLTKLGVEVEVLDE
                     KQMKKLGMGALLGVGQGSARPSRLAVMQWKGGPKGAKAAPVAFIGKGVTFDTGGISIK
                     PADGMWDMKWDMAGAGVVIGLMKALAGRKAKVNAVGVVGLVENMPSATAQRPGDIVTS
                     MSGQTIEVLNTDAEGRLVLADALWYCQDRFKPQFMIDLATLTGAIIIALGSENAGLFS
                     NNDELAERITAAGRGVGESVWRLPLAEPYDKLMDSDAADIKNISGGREAGSITAAQFL
                     QRFVNGVPWAHLDIAGTTWSKKDAATVPKGATAFGLRLLERLVAEHYES"
     gene            4234..5148
                     /locus_tag="VGP50_00325"
     CDS             4234..5148
                     /locus_tag="VGP50_00325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV8025836.1"
                     /translation="MSGVSFIVTVYNKKEFLPRVLHALGAQTGDFFREYVFVDDGSTD
                     GSLDYLRSATVEWPHCKIIAQRNRGASAAMNSAVDAATQDYLKLVDADDVLVPDATRW
                     LLTALQSANAVLAYGDAGSYVPGQTIPWPAAPQLPTSELIQAPLHPTIRGSTFNPSKM
                     LMARSDYRRVGGADETVCCQDYSLALPLARLGSFVRVRAIVMLAPTVAPGRLSENHAR
                     ELHDVTRALATFVERHADLPRAEKAYAVRRAAGRAMLWSRRHGDVANAVRFGLVSLAA
                     RLNFVRDHAAAIKACCSAYPYDPPVPIG"
     gene            complement(5188..7050)
                     /locus_tag="VGP50_00330"
     CDS             complement(5188..7050)
                     /locus_tag="VGP50_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025837.1"
                     /translation="MAPLIPGWTALGDWAGVLIAGVALIGLGRLLLLGRVTPEAALVA
                     GWGGAALALTFWGVATTASLRLPGAALLALGLLALLVPRLRLTWADWRSLGRIVAVAL
                     PLLAVMASARPSLPDTFLNLLPNAAYLYDHATFPADDRAPSYSFLPAAPYNLQLAAFL
                     ASLFTGRFPPSAMIGLNILLQIAFALFLARIIQHGEGEEPGPPAWTAAALALLLTTAL
                     NPGFVPRYDLSSYSEASVTVTVALAAWFAARALDDLAAARRSTVSLGLLALTLAALVN
                     IKQDSVAIAAGVVITAGVLAAAAPAERRSRSLTAVALAAAPAVALYLAWRWYVIAHFA
                     VGELKPLPLGQWQFANIPLILRSMAGVMVEKPYFFGLAALTVAFLAWRLWQRGLDRTS
                     RVAALFAGVALVYNSALFGAYIAHFPGEMSSDAHSYYRYNTHLGLLLVLALVLLARAA
                     VAERALGLSGSARRAVLPVLAGALLLCPVVFLRQLRFDLEEPQQRAWQLAALASRALA
                     EEHRLAMLLPGDNGSLAAMLEGLIRFTPPRHLDADLGAITVLGPDTLGQLAAQGYRRA
                     LVSCLPAPPGDIVPPQAALLARGPAGWDAVALSPYQPPHSHRWSHVIAAAPLCL"
     gene            complement(7079..8599)
                     /locus_tag="VGP50_00335"
     CDS             complement(7079..8599)
                     /locus_tag="VGP50_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025838.1"
                     /translation="MPAFGRFAGVFLALFVVAAIPVLSCDSMPLFDYPNHLARMHILL
                     DLPDSPTLQQFYEVAWRPLPNLAMDAIVPVLGRVMPLAWAGTVFVLLTLFLLACGAAI
                     LHRVLFGAWSAWSCLAFLLLYSRTLLWGFLNYLFGLGLALLALALWVALARRPMALRL
                     VAGVFLGLALFFAHLLAFGLYGVMVMSYEAGRILRGGVSIRQGLAEIVIAGLGFVPAL
                     GILFLGSPASMSGAVRWGHLARKIDLLFSVFDNYDRGFDVACFVLVVLALLFVFWRGW
                     VRIAPDIAVPLGLLVVVYLAMPSQLATASGSDHRIPLLIGFVLIAGSRWVGPAAPQRL
                     FLAAALLMFLLRLAVLTASWTESDRTYANLLPALDAIPVGGRVAVAYPAEALNSEVAP
                     LAHFPTLAILRRDAFVPTLFAFPTQQPVRLRPAYRLLADRLPPERLWAAFVAGDAPLD
                     GEDRAAFAAYDDIVFVGRAPFSLAASPGLTPVFTAPRFALFRIDPGARLDAPGPSR"
BASE COUNT         1639 a         2891 c         3021 g         1202 t
ORIGIN      
        1 accggccgcg gcgccgtgct cgagagctgg cgcagcatcc ttcaaaaccc ggaagcaccg
       61 catgtgatgt gccatgacga taccgccttc ctccacggcg acgtcgccat cgtgctttgc
      121 gaggaagagc tggcggacgg ctctctctcg gccaccaaca tcttcgtcaa ggaggatggc
      181 gagtggcgcc tcgtccacca ccaggcgagc cagctcctcg cccgcggcag cccggcggcg
      241 ccggcatgaa gcatcgccgg gggcggcgga gccgatatgt cagcgcgcga ccggtgacgg
      301 cgggccggtg atcgacgccg cgtaagcggc gccggtgagc cagagagcgg ccagccacca
      361 ctcctgccag gcgccgaagc tcagcatcgc gaagaccaag gccgcagccg cccaggcgag
      421 cgcgccggcg cgttcgcggg gcgagaggtg cttttgccgg ccgagcctcc atagcgcagc
      481 gatgatggtg gcaagagcca agagcgcccc cggtatcccc aactccacct cccattggag
      541 gacggcattg tgcgggtgaa gcggcagcgc ctcggcatcg ggaccaagcc cggcctcggg
      601 cagcgcctga ccaaaattga aatggccgcc gggcaacgct cgggaggcat cgacgcccca
      661 gccgaggatc ggccgctcgg cgatgcggtc ggcggtgaag cgccagatca gcaggcgatg
      721 aatccccgag ctcttgatcc acggcgcccg ctcatgcatc gcgaccgcgg tcgctgcgtc
      781 cggcaccgcc aaggggagca gaatggcgaa gaggatcagg cagccgccca gggccgccgc
      841 gacgacgcgc gggacatgcc aggcgatcag ggccacggca atgctgacga gcaaggcgag
      901 ctttgccgcg gcgctgacca tcgccatcac ggccacgacg acggccgccg caacggccag
      961 ggccgtcccg atccgccgct gagcggccat cgccggccac aggaacagca ccatgacgac
     1021 cgccccgcga tcaaagcgcg acaagggcag ctcgatcccg accggtggcg tgacgaccat
     1081 acgggtcagc gcggcaccgc tgaagcgctc gacgagaatg agaactgcgg caatgacgac
     1141 gccagccgtc gccgccctgc ggactgagtc tcgaccaatc ggctcgagcc gggtggccgc
     1201 gccgaacacg agcagcccgg cgacgctgat cagcgcgaag cgcagcgcga cgacaaggct
     1261 atgctcgggc agaatcgacc agagcgccga cgcaaggcac cagagcgaca gcgcggcaag
     1321 cagagccacg agccacagca tgccggggag tgtttcaagg tgacgccggc cgtccatgac
     1381 gagcacgacc agcgccacga ggccaagcaa gggcgccaat ccgtgcggcg cgaaaaccgc
     1441 gagcgacggg gcaagcagcg cagcgccggc gagcgcgcgc ccgctgtcga gagcgtttgc
     1501 tctggacacc cgctacttga gatcggggaa aaagaaggcg atctcgccgc gcgccgtctc
     1561 gagcgaatcg gagccgtgca cggaattcgc ttcgatcgat tcggcgaaat ccttgcggat
     1621 ggtgccgggt gccgctttcg ccggatcggt cgcgcccatg acctcgcggt tgagcgcgac
     1681 cgcgttctcg ccttcgaata cctgcgccac gacgggcccg gagatcatga acttcacgag
     1741 atcgttaaag aaggggcggc tcttgtgcac ggcgtagaat ttctccgcct gctcgcggct
     1801 cagcgtcagg cgcttgtcgg cgacgatgcg caatcctgcc tgcttgaagc gggcgttgat
     1861 cttgtcggtc aagttgcgcc gcgtcgcgtc gggcttgatg atcgacaagg tgcgttcgac
     1921 ggccatggcg ggtcgacctc tttcgctatg cgctaagcgc ccgaggtata gccgcctccg
     1981 ccgcgcccat caacccgcct cggcggtacc ggcgccccgc ttctcccact ttccctcgct
     2041 ctgctcccaa taaacgaggc ggtgaccggc agccttggcc tcgcgccagc gctcgcgagc
     2101 cgcggtgacc gcagcctcgt cattgccatc gaacatgtcg aagcagcgcg cataagcgtc
     2161 gagccgcgcc gagcaggcgc cgtcgaccag caccagcatg gtggcgccat tcggattctc
     2221 gtcggcggtg gtgagccaga cgggttggcg gtcgccgctg ccgtcccgcg cggtgccatg
     2281 gggaaggaag ctcgcgtcgt catacgtcca gagcagcgcg tcgagatggt cgacccgctc
     2341 ggccgagccg gccatcacca cgacccggtg accctcggtg agcgcacgct cgaggatgcg
     2401 cggcagcgcc cgctcgaggg gcgtgctgcg cagatgatag aagccgacct ccgtcacgac
     2461 tcgtagtgct ccgccaccag ccgctcgaga aggcgcaggc cgaaggccgt ggcgcccttg
     2521 ggcacggtcg ctgcatcctt cttcgaccac gtggtaccgg cgatgtcgag atgcgcccag
     2581 ggcacgccat tgacgaagcg ttgcaggaac tgcgcggccg tgatgctgcc ggcctcgcgg
     2641 ccgccgctga tgttcttgat atcggcggcg tcgctgtcca tcagcttgtc atagggctcg
     2701 gcgagcggca ggcgccacac cgactcgccc acgccccggc cggcggcagt gatccgctcc
     2761 gcgagttcgt cattgttgct gaaaagcccg gcgttctcgc tgccgagcgc gatgatgatc
     2821 gcgccggtga gcgtggcaag gtcgatcatg aattgcggct tgaagcggtc ctggcagtac
     2881 cagagcgcat cggcgaggac cagccggccc tcggcatcgg tgttgagcac ctcgatggtc
     2941 tggccggaca tcgaggtgac gatgtcgcca ggccgttgcg ccgttgccga gggcatgttc
     3001 tcgaccaggc cgaccacgcc gacggcgttg accttcgcct tgcgcccggc cagcgctttc
     3061 atcaggccga tgacgacgcc ggcgccggcc atgtcccatt tcatgtccca catgccatcg
     3121 gcgggcttga tcgagatgcc gccggtgtcg aaggtgacac ccttgccgat gaaggcgacg
     3181 ggcgccgcct tggcgccctt gggaccgccc ttccattgca tgacggcgag ccgcgacggg
     3241 cgcgcgctcc cctgccccac gcccaggagc gcgcccatgc cgagcttctt catctgcttt
     3301 tcgtccagca cctcgacctc gacgccgagt ttggtaagcg tgcgcgccgc ctcggcaagc
     3361 gtctcgggat agatgatgtt ggccggctcc gagacgagat cgcgggtgag gaagacgcca
     3421 tcggccacct tgtcgagcgg cgtgaagccg cgcttcgcct ctgcgacatg atcggtcatc
     3481 accttgagct cgcgcagcga cggcttctgc tcgggcttct ccttggtgcg gtatttgtcg
     3541 aagcgatagg cgcgcagccg cgccccatag gccagattcg ctgccgcctc gcctggcttg
     3601 aagttcatgc cgggaatgtc atcgagatcg acggccgctt ccttctcgcc ggcgctgttg
     3661 agctgcgcga cgatctgccc gccgaggctc tgcatcgcgc cggcatcgag cgcctgcgcc
     3721 ttgccgaggc cgatcagcag cacgcgcccg acctcgagct tcgccggggc aagcacgccc
     3781 aggatctcgt ccttccggcc cgtgaagcgg ctggcggcca tcgcacgctt gagtgctccg
     3841 ccgctctcct tgtccagcgc cgcagcccgc ggcgtcagtt tgcgatcctc gagcacgccg
     3901 accacgatcg tgcccgagtt gggctcccgc ggctcggcaa aggcgatctt catcatgact
     3961 cccccgtcgg tgttcccccg ccgatagacg caaccgcttc gagtgtcaac cagggtcggc
     4021 ggcgccgggt ttggctggct gctgaagaag tcgccaaaga taaaccttga aagcggcccg
     4081 caccggtcgg cgggcgatcc ttttcaaatt cagcggagcc gcaggcttcg cccgctggcc
     4141 gccaaggaag gtccgccacc ccgcctcttt gcgacgcatg cgaaaaggtg tatttgagag
     4201 ttctacgagt catcgggagt tcaaaagaag aaaatgagcg gcgtttcgtt catcgtgacg
     4261 gtctataaca agaaggaatt cctgccgcga gtgctgcatg ctttaggcgc ccagaccggc
     4321 gattttttcc gcgaatacgt ttttgtcgat gacggctcga cagatggctc cttggactac
     4381 ttgcggtccg cgaccgttga atggccgcac tgtaagatta ttgcccaacg aaatagaggt
     4441 gccagcgctg caatgaacag cgccgtcgat gccgcgacgc aagattatct gaagctggtg
     4501 gatgccgacg atgtcctcgt tccggacgcc acgcgatggt tgttgaccgc cctgcaaagc
     4561 gccaatgccg tgctcgctta cggcgacgcc ggttcctatg ttccggggca gacgattccg
     4621 tggcccgcgg ctcctcaatt gccgacatcc gaattaatcc aggcacccct gcatccaacc
     4681 atacgcggca gtacgttcaa cccaagtaag atgctcatgg cgcgctccga ctatcgccgc
     4741 gtcggcggcg ccgatgagac ggtttgctgc caggactaca gcctcgcatt accattggcg
     4801 cggctcggca gcttcgtgcg cgtccgcgcc atcgtgatgc ttgccccgac agtagcccca
     4861 ggccgtctct cggagaatca tgcacgggaa ttgcacgatg tgacgcgcgc cttggcgacc
     4921 ttcgtcgagc gtcacgcgga tctgccgcgt gccgagaaag catacgcggt aaggcgcgcc
     4981 gctggacggg cgatgctttg gagtcgtcgg cacggcgacg tcgcaaacgc cgttcgcttt
     5041 ggccttgtgt cactggcagc gcggctaaat ttcgttcgcg accacgcggc cgcgatcaag
     5101 gcgtgctgca gcgcctaccc ctacgatccg ccggtgccga tcgggtagcc cgcgcgcaga
     5161 gccccgtttc tcctgtatca agacgcttca gaggcagagc ggcgccgcgg cgatcacatg
     5221 cgaccagcga tgcgaatgcg gcggctgata aggcgagagc gcgaccgcgt cccaaccggc
     5281 cggtccgcgc gcgagcaacg ccgcttgcgg cggcacgata tcacccggcg gcgctggcaa
     5341 gcaggacacg agggcgcgcc gatagccctg cgccgccagc tgtccgaggg tgtcggggcc
     5401 gagcaccgtg atcgcgccga gatcggcatc gaggtgacgc ggcggcgtaa agcggatcag
     5461 gccctcgagc atggcggcga gactgccatt gtcgccggga agcagcatcg ccagccgatg
     5521 ctcctcggcg agcgcgcgag aggcgagcgc ggcgagctgc caggcccgtt gctgcggctc
     5581 ctcgagatcg aagcgcagct ggcgtaggaa gaccaccgga cacagcagga gcgcgcccgc
     5641 cagcaccggc agcacggcgc ggcgcgcgct gccgctgaga ccgagcgcgc gctcggcaac
     5701 ggcagcgcgg gcgagcagca ccagggccag gacgagcagc aagccgagat gggtgttgta
     5761 gcggtaatag gaatgggcat cgctgctcat ctcgcccggg aaatgcgcga tataggcgcc
     5821 gaagagcgcg ctgttataga caagggccac cccggcgaag agcgcggcga cgcggcttgt
     5881 ccgatcgagc ccgcgctgcc agagccgcca agcgaggaaa gcgaccgtca gtgcagcgag
     5941 gccgaagaaa tagggcttct cgaccatcac tccggccatg ctgcggagga tgagcgggat
     6001 gttggcaaac tgccattggc caagaggcag cggcttcagc tcgccgaccg cgaaatgcgc
     6061 gatgacgtac cagcgccagg caaggtacag cgccacggcc ggcgccgcgg cgagcgccac
     6121 ggccgtgagc gagcgcgagc gccgctcggc gggcgccgcg gcggccagga cgccggccgt
     6181 gatgacgacg cctgcggcga tggccacgct gtcctgcttg atgttgacga gcgccgccag
     6241 cgtcagagcg agaaggccga gcgacaccgt gctgcggcgc gcggccgcga gatcatcgag
     6301 cgcacgcgcc gcgaaccagg ccgcgagggc gacggtgacg gtgacggagg cttcgctgta
     6361 gctggagaga tcgtagcggg gcacgaaacc gggattgagc gccgtcgtga gcagcagcgc
     6421 gagcgcggcg gccgtccagg ccggcggacc aggctcctcg ccttcaccgt gttggatgat
     6481 gcgcgcgagg aaaagcgcga aggcgatctg caggaggatg ttgagaccga tcatggcgct
     6541 cggcggaaac cggccggtga agagggaggc gaggaaggcc gcgagctgaa ggttataggg
     6601 agcggccggc agaaacgaat agctgggtgc acggtcatcg gcggggaacg tcgcgtggtc
     6661 gtagagatag gccgcgttgg gcaggagatt gaggaaggtg tcgggcaggc tcggtcttgc
     6721 gctcgccatc accgcgagaa gcggcagcgc cacggcgacg atgcgcccga gcgaccgcca
     6781 atccgcccag gtgagccgca accgcgggac gagaagcgcg aggaggccca gcgcaagcag
     6841 cgccgcgccc ggcagtcgca gcgatgccgt cgtcgccacg ccccagaacg tcagcgcgag
     6901 agcggcgccg ccccagccgg cgacgagcgc ggcttcgggg gtgacgcggc cgagcagcag
     6961 caaccgcccg aggccgatca gcgcgacgcc ggcgatgagg acgccggccc agtcgccaag
     7021 cgcggtccaa ccggggatca gcggtgccat ggggggataa aaggcaagcg cgcggggtct
     7081 atcgggaggg accgggcgcg tcaaggcgag cgccggggtc gatgcgaaag agggcgaagc
     7141 gcggcgcggt gaagaccggc gtcaatccgg gtgacgccgc gagcgaaaaa ggcgcccgcc
     7201 cgacgaagac gatgtcgtcg taagcggcga aggccgcgcg gtcctcgccg tcgagcgggg
     7261 cgtcgccggc gacgaacgcg gcccagagcc gctccggggg gagccggtcg gcgagcaggc
     7321 gataggccgg acgaagccgg accggctgct gcgtcgggaa ggcgaagagc gtcggcacga
     7381 aagcgtcgcg gcgcagaatg gcaagcgtcg ggaaatgcgc gagcggcgcc acctcggaat
     7441 tgagggcctc ggccggatag gcgacggcga cacggccgcc gacggggatc gcatcgagcg
     7501 ccggaaggag gttcgcgtag gtgcgatcgc tctcggtcca gctcgccgtc aggacggcga
     7561 gccgcagcag gaacatgaga agcgctgcgg caaggaagag acgctgcggc gcggcagggc
     7621 cgacccagcg gctcccggcg atgaggacga agccgatcag caaggggatg cgatgatcgc
     7681 tgcccgatgc cgtggcgagc tggctcggca tcgccagata gaccacgacc agcagcccga
     7741 gcggaacggc gatgtcgggc gcgatgcgca cccagccgcg ccagaaaaca aagagcagcg
     7801 ccagcacgac gagcacgaag caggcgacat cgaagccacg gtcatagttg tcgaagacgc
     7861 tgaacaggag atcgatcttg cgcgcgagat ggccccatcg cacggcaccc gacatgctcg
     7921 cggggctgcc gaggaacagg ataccgagcg ccgggacgaa acccagaccg gcgatcacga
     7981 tttcggccag gccctgccgg atcgacaccc cgccgcgcag gatccgcccc gcctcgtagc
     8041 tcatcaccat cacgccgtag aggccgaagg cgagaagatg ggcgaagaac agcgccagtc
     8101 ccagaaaaac gccggcaacc aggcgcagcg ccatcggccg ccgcgccagc gccacccaca
     8161 aggcgagcgc caggagagca aggccgagac cgaagaggta gttcaaaaaa ccccaaagca
     8221 aggtgcggct gtagagcagc aggaaggcga ggcaggacca cgccgaccag gccccgaaga
     8281 gcacgcgatg gaggatcgcc gcaccacaag ccagcaggaa cagggtcagc agcacgaaga
     8341 ccgtgccggc ccaggcgagc ggcatgacgc ggccaagaac cggcacgatc gcgtccatcg
     8401 ccagattggg gagaggtcgc caggcaacct cgtagaattg ctgaagcgtc ggcgaatcgg
     8461 ggagatcgag caggatatgc atgcgcgcca gatgattcgg ataatcgaac agcggcatgc
     8521 tgtcgcagct gaggaccggg atcgccgcca cgacgaagag cgccaggaaa actccggcaa
     8581 atcgcccgaa ggccggcatc gaagaatcgc cactctgatg aatccgcacc ggcgctccct
     8641 tttgcccgat cggccccgcg gagagttcca cgcaatacac gccgccgcaa gcggatgatc
     8701 cgacgcaaac ttcgctctag gctcggcggc gaaggtccct ataatcggcc gca
//
LOCUS       DASYNS010000010         7170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4527326, whole genome shotgun sequence.
ACCESSION   DASYNS010000010 DASYNS010000000
VERSION     DASYNS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4527326"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VGP50_00340"
     CDS             <1..807
                     /locus_tag="VGP50_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEV8025839.1"
                     /translation="VHAEIDGHDAEIAGLHKRTVFLEEEMAGVRQLVAKGLERRPRLL
                     QLERDMAEMEGKIGDTTAQIAKAKQSIAESEVDILSIQNDHQKDVADELRETQKKRHE
                     LEEQAQAAADVLARVEVKAPESGIITDLRVHTPGGVVAAGEPLLDLVPDADRLIVEAQ
                     VRPEDIDRVHEGLPAQVRLLPYKQRRTPPLDATVIYVSADRLVDKHTNMPYFVAKLRV
                     DEEALGALGSDVKMVPGMPSEAMIQTGASTVAIYALSPLLDSFHRAFREK"
     gene            1087..1440
                     /locus_tag="VGP50_00345"
     CDS             1087..1440
                     /locus_tag="VGP50_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV8025840.1"
                     /translation="MAELLVVMNDDRVTARFVRFLIAEGVIPAPRGGRANADYGDDHE
                     SGIRRYLQLRDLGLSASRTKEIVSGTASGGIPVPIAPGLTLIVDQEKISQPPHPHDIA
                     ATIAEALNLIVPRDN"
     gene            1442..3472
                     /locus_tag="VGP50_00350"
     CDS             1442..3472
                     /locus_tag="VGP50_00350"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEV8025841.1"
                     /translation="MPISPNPLARRGVIDGAGRRDRTPIPLASERMRVVIRDNLALVS
                     REQTFRNSEERSIEATLTFPVPIHATLHRLVAKIGERTLTGSAMPRAKARHAYEKAVD
                     DGRTAVLHEELVRGIHMLSIAHIPPGSEVKVTHSWISAMSPRGAGKAVLRVPVNLGDV
                     YGDSPFSDADDLSTSPEVIHEADVEIDARAFTASIRGVPVVDSTIRLRLDAPIGVEVN
                     GFSNTPAHGVSADGRSVQVLVVPDRGGDAAIDAAILVDRSGSMGDAASSKQPGLTKHA
                     AIAAGLVDVARALRPTDRAELWQFDNSCERLSDPEEPLIAAVSRLDEPRGGTEIGAAL
                     KSVVAGCEARDIVLITDGLSHALDVQTLVNSGHRFTVVLVGEDSLEANVGRLAALSGG
                     QIVLATGGADATAAVGMALASVRRPKPSFDLQTWPLARAAIHAGGAVIEASWEDRPVA
                     PIEPEFGTAVGALAAAIALPLLREDAAIKVAADHRILCHLTSLVLVDEAGTVQTGLPA
                     QRKIPLMAAPQVSFALGRSKMVRCERDRGTLNSRGGDVRTLTAHEHAGRTASSREGDI
                     RTLSKAARQIEWERDPEALRRGDLNALPAGVARVVASARWRPAIVALAEAIHGEVTAI
                     VIALMALSVSSSSRSAERIARAVLAGADDNMLSRAMAVLGLHHAKAGNTARV"
     gene            complement(3469..4362)
                     /locus_tag="VGP50_00355"
     CDS             complement(3469..4362)
                     /locus_tag="VGP50_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV8025842.1"
                     /translation="MHRLDDLRAFVAIVDRGSLTNAARGLRRSLQSVSRSLAALEREI
                     GVELVRRTTRRSAPTEAGIALFRRVKAALAEIDAAALDAADKHAEPAGLLRISSSSAF
                     APLYLIPVIVAFLGEHSAVELELELSDAYVDLVERGFDLAVRIGALPESSLVAKRLAD
                     LRRVVFAAPDYFKRHGRPKQPADLARHQCIIRTAARENDAWPFTIDGRLRKIKVGGRF
                     RTSGAIAANEAAALGLGIANAPLWQVRPLLDQGRVELALTRFEPPPIPLNAVWPASRV
                     LPAKTRLFVDFLATELARQKF"
     gene            4474..5004
                     /locus_tag="VGP50_00360"
     CDS             4474..5004
                     /locus_tag="VGP50_00360"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEV8025843.1"
                     /translation="MTRAFTAFAFSPSVKAAQARHGVREHGARLEQKEPANDRLTPDL
                     MRYVAETDSFFIATASREGWPHVQHRGGPAGFLRVIDDRTLAFADYAGNRQLITVGNL
                     AENDRVMLFIIDYERGRRLKIWGRARIIDEDPALFERASDPGCPAKVERVIRIDVKAF
                     DLNCRQHFPKLVRVKS"
     gene            complement(5104..5670)
                     /locus_tag="VGP50_00365"
     CDS             complement(5104..5670)
                     /locus_tag="VGP50_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEV8025844.1"
                     /translation="MNWVAIIVGLVALQRLGELVVATRNTRTLLNRGGVEHGARHYPL
                     IVLLHAAWLVALLFLVPADAPISWFWLAVFLLLQALRLWVVASLGPYWTTRIITLPEE
                     PLVRRGPYRFLRHPNYAVVAGEIAVLPLVFGAWGIALVFSLLNAAMLAWRIHIEEQAL
                     AQRRTRNIESTHAQSSPLPLREREGPAA"
     gene            complement(5667..6719)
                     /locus_tag="VGP50_00370"
     CDS             complement(5667..6719)
                     /locus_tag="VGP50_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEV8025845.1"
                     /translation="MTTTIPRLASLSTAVPPHVLGQPEVTIRARHLFAERAGAALDRL
                     MPVFANAGIERRYSSVPIDWYEKPHGWTDRNALFLENAVNLLEETSRDALALAGLTPG
                     DIDAVVVASTTGVATPSLDAMLINRLGLKPEVRRLPIFGLGCAGGVIGLARAADLARI
                     HPTSRVLFAVVELCALNFRKNDTSKSNVVATALFGDGAAAAVLSCRGDGPALGPAGEH
                     TWPNSLDIMGWDVVEDGMKALFSRDIPALVRGHMRQVAGDFLARHGLSLENIRHFVCH
                     PGGAKVLTALEEAFHLAEGALADARGVLRDYGNMSAATVMFVLERMLRQGATGRMLVT
                     ALGPGFTAGFQLIDAG"
     gene            6813..7160
                     /locus_tag="VGP50_00375"
     CDS             6813..7160
                     /locus_tag="VGP50_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025846.1"
                     /translation="MFEGFTTARLATAGAKIHLRQGGKGRPLLLLHGYPGTHVMWHKI
                     VTRLARRFTVVASPLLVLWGGKGVVGRLYGSLALWREKAVDVTGRVVPCDHFIPEEAP
                     EEPLAALAAFLAG"
BASE COUNT         1268 a         2355 c         2435 g         1112 t
ORIGIN      
        1 gtccacgccg agatcgacgg ccatgacgcc gagatcgcgg gcctgcacaa gcgcacggtg
       61 tttctggagg aagagatggc cggtgtccgc cagctcgttg ccaagggtct cgagcgcagg
      121 ccgcgccttt tgcagctcga gcgcgacatg gcggagatgg agggcaagat cggcgacacc
      181 acggcgcaga tcgccaaggc gaagcagagc atcgccgagt ccgaagtcga cattctgagc
      241 atccagaacg atcaccagaa ggatgtcgcc gacgagctgc gcgagacgca gaagaagcgc
      301 catgagctcg aggaacaggc gcaggccgcc gccgacgtcc tggcgcgcgt cgaggtgaag
      361 gcgccagaaa gcggcatcat caccgacctc cgtgtccaca cgccgggcgg cgtcgttgcc
      421 gccggcgagc cgctgctcga cctcgtgccc gatgccgacc gcttgatcgt cgaagcgcag
      481 gtgcggcccg aggatatcga ccgcgtgcat gaaggcttgc cggcgcaggt gcggctcctg
      541 ccctacaagc agcggcgcac gccgccgctc gacgcgacgg tgatctatgt ctcggccgac
      601 cgtctcgtcg acaagcacac gaacatgcct tacttcgtcg cgaagctccg ggtcgacgag
      661 gaagcgctgg gcgcgctcgg cagcgacgtc aagatggtgc cgggcatgcc gagcgaggcc
      721 atgatccaga ccggcgccag caccgtcgcc atctacgccc tctcccccct cctcgacagc
      781 ttccaccgcg ccttccgcga gaaataggcg cagcacatcc ggaagagcga aaaactcccg
      841 gcgcgaagcg ccgggagttt gaccgactcc cggcgaagaa acttcgacga tgcggcccaa
      901 acggcgctgt ctcctggcgc cgcaccgctt cgtgaagggc caggctctcc cgccgccggc
      961 agaggaacgt tcgcccgcct catccctccg gaactcatac gacagttttg gtgtcgaaag
     1021 gccttgacaa tccgacgcgt cttgcgtcta atgacatcga tactgtcaaa agagcgcgcc
     1081 gtgacgatgg ccgagcttct cgtggtgatg aacgacgata gggtgactgc ccggttcgtt
     1141 cggttcctca tcgccgaagg ggtcatacca gcgccgcggg gaggccgagc caacgctgat
     1201 tatggcgacg atcatgagag cggcattcgc cgctatctgc aattgcgcga cctcggtttg
     1261 tccgcatcgc ggacgaaaga gatcgtctcc ggaacggcga gcggcggcat accagtgccg
     1321 atcgcgccgg gtctgacctt gatcgtcgac caggaaaaaa tcagccagcc gccacacccg
     1381 cacgatatcg ccgcgacaat cgccgaagcc ctcaacctta ttgtgcccag ggacaattga
     1441 aatgcccatc agtccgaacc cgctcgctcg acgcggcgtc atcgacggtg ctggccgtcg
     1501 cgatcgcacg ccgataccgc tcgcgagcga acgcatgcgc gtcgtcattc gcgacaacct
     1561 cgccctggtg tcgcgcgagc aaaccttccg caacagcgag gagcgcagca tcgaggcgac
     1621 gctcactttc ccagtcccga tccatgccac tctgcatcgc ctcgtcgcga agatcggaga
     1681 gcggacgctg actggctcgg cgatgccacg cgccaaggcg aggcacgctt acgagaaggc
     1741 ggtcgacgac ggccgaacgg cggtcctcca cgaagagctg gttcgcggca tccatatgct
     1801 gtcgatcgca cacatcccgc cgggcagcga agtgaaggtg acgcatagct ggatatccgc
     1861 gatgtcaccg cgcggcgccg gcaaagccgt gctgcgcgtt ccggtcaacc tcggcgatgt
     1921 ctatggcgat tctccgttta gcgatgccga cgacctcagc acctcgccgg aggtcattca
     1981 tgaggccgat gtcgagatcg atgcccgcgc ctttacagcg tcgataaggg gcgtcccggt
     2041 agttgatagc accatcaggc ttcggctcga tgcgccgatc ggtgtcgagg tcaacggctt
     2101 ttcgaatact cccgcccatg gtgtatcggc cgatggccga agcgtgcagg tgttggtcgt
     2161 gcccgatcgc ggcggcgatg ccgcgatcga tgcggctatt cttgtcgatc gctcaggatc
     2221 gatgggcgac gcggcttcgt cgaagcagcc gggtctcacc aagcatgctg ccatcgccgc
     2281 aggattggtc gatgtggcgc gcgcgctcag gccgaccgac cgtgcggagc tgtggcagtt
     2341 cgacaatagc tgcgagcgcc tctccgaccc ggaggagcct ttgatcgccg ccgtgtccag
     2401 gctcgacgag ccgcgcggcg gcacggaaat cggcgcggca ctgaagagcg tcgtcgccgg
     2461 gtgcgaggcg cgcgacattg tcctcatcac cgatggcctg agccatgcgc tcgacgtcca
     2521 gacgctcgtc aattcaggac atcgcttcac cgtggttctt gtcggcgagg actcgcttga
     2581 ggccaatgtc gggcgacttg ccgcgctttc cggcgggcag atcgttttgg cgacaggtgg
     2641 tgcggatgcc actgccgcgg tcggcatggc gctcgcatcc gtgcgacgac caaaaccctc
     2701 cttcgatctc caaacctggc cgctcgcccg cgcggcgatc cacgccggcg gcgccgtcat
     2761 cgaggcgtcc tgggaggatc gtcctgtggc gcctatcgag ccggaattcg gaaccgccgt
     2821 cggcgctctg gcggcggcga tcgctctccc gttgctccgc gaagacgcgg cgatcaaggt
     2881 ggcggcggac catcggattc tttgtcacct gacgagcctg gtgctggtgg atgaggcggg
     2941 aacggtccaa acgggtctgc cggcgcagcg caaaattcct ctgatggcgg ctccgcaagt
     3001 gagttttgcg ctgggacggt cgaaaatggt tcgctgcgaa cgcgacaggg gaacgctcaa
     3061 ctcgcgaggg ggcgacgtgc gaacgctcac agcgcacgag catgccgggc gcaccgccag
     3121 ttcgcgagag ggcgacatac gaacgctcag caaagccgcg cggcagatcg agtgggagag
     3181 agatcctgag gcgttgcggc gtggtgatct caacgcgctt cctgccggtg tcgcgagagt
     3241 cgtggcgagc gctcgctggc gtccagcgat cgtcgcgttg gccgaggcga tccacggcga
     3301 ggtgacggcg attgtcattg ccctcatggc cttgtccgtc agctcttcaa gccgatcggc
     3361 ggagcggatc gcgagggcgg ttctagcagg agcggacgat aacatgctct cccgcgccat
     3421 ggccgtcctc ggcctccatc acgcgaaggc tggaaacacg gcccgggtct agaacttctg
     3481 cctcgcgagt tcggtcgcaa ggaaatcgac gaacagccgc gttttcgcgg gcagcacgcg
     3541 gctcgccggc cagacggcat tcagcgggat cggcggcggc tcgaagcgcg tcagcgccag
     3601 ctcgacacgt ccctgatcca gcaaggggcg cacctgccag agcggcgcat tggcgatgcc
     3661 gaggcccagc gccgccgcct cgttggcggc gatggccccg ctggtgcgga agcgcccgcc
     3721 caccttgatc ttgcgcagcc tgccatcaat cgtgaagggc caagcgtcgt tctcgcgcgc
     3781 ggccgtgcgg atgatgcatt ggtggcgggc caagtccgcg ggctgcttcg gccggccatg
     3841 ccgtttgaag tagtcgggcg ccgcgaagac gacgcgccgg agatcggcaa gccgcttcgc
     3901 cacgaggctc gactccggca acgcgccgat acgcacggcg agatcgaagc cgcgctcgac
     3961 gaggtcgaca taggcgtccg acagctcgag ctcgagctcg accgcggaat gctcgccgag
     4021 gaaggcgacg atgaccggga tcaagtagag cggcgcgaag gccgaggagc tgctgatgcg
     4081 cagcaggccc gccggctcgg cgtgcttgtc ggcggcgtcg agcgccgccg cgtcgatctc
     4141 cgcaagcgcc gccttgacgc ggcggaagag cgcgatgccc gcctcagtcg gcgccgagcg
     4201 ccgcgtcgtc cgccgcacca gctcgacacc gatctcgcgc tcgagcgcgg cgagcgaccg
     4261 gctcacggat tgaagggaac gtcggagccc gcgcgcagcg ttcgtcaggc tgccgcgatc
     4321 gacaatggcg acgaaggccc ggagatcgtc gaggcggtgc atcggcattc tctcgcaaag
     4381 cggaacaatc tatcccgccg gcaccatatt atcaaaacgg gaccgagccg ccatctcatg
     4441 ggcgcggacg caacgatcag ggagcaaagg ctcatgacgc gcgctttcac ggccttcgcc
     4501 ttttcgcctt ctgtcaaagc cgcacaggcc cgccacggcg tgcgcgagca cggcgcgcgg
     4561 ctcgagcaga aggagccggc caatgaccgg ctcactcccg acctcatgcg ctacgtcgcc
     4621 gagaccgaca gcttcttcat cgcgacggcg agcagggaag gctggccgca tgtgcagcat
     4681 cgcggcggcc ccgcgggatt cctcagggtg atcgacgatc gcacgctcgc cttcgccgac
     4741 tatgccggca accggcaatt gatcaccgtc ggcaacctcg cggagaacga ccgcgtcatg
     4801 ctcttcatca tcgactacga gcgcggacgt cgtctcaaga tctgggggcg cgcccgcatc
     4861 atcgacgagg acccggcgct cttcgagcgt gcgagcgatc ccggttgtcc ggcgaaagtc
     4921 gagcgcgtga tccggattga cgtgaaggcg ttcgacctca actgccgcca gcacttcccg
     4981 aagctggtgc gggtgaaatc gtgaccatga aatccctctc cctttgcggg agagggaggg
     5041 gcccattgcg aagcaatggg agggtgaggg gttcgtgcgc gcttacccct caccctcccg
     5101 ctgctacgca gcgggcccct ccctctcccg caaggggaga ggggagcttt gggcgtgcgt
     5161 ggattcgatg ttgcgcgtcc ggcgctgcgc gagcgcctgc tcctcgatgt ggatgcgcca
     5221 cgccagcatg gccgcgttga gcagcgagaa gacgagggcg ataccccagg cgccgaatac
     5281 cagcggtagc acggcgatct cgccggcgac caccgcgtag ttcggatggc gcaggaagcg
     5341 gtaggggccg cgccgcacca gcggctcctc gggcagcgtg atgatgcgcg tcgtccagta
     5401 aggaccaagg ctcgccacca cccagagccg caatgcctgc agcagcaaaa agacggcgag
     5461 ccagaaccag gaaatcggag cgtcggccgg cacgaggaag agcagcgcga caagccacgc
     5521 cgcatgcagc agcacgatca gcggataatg gcgggcgccg tgctcgacgc cgccgcggtt
     5581 caagagcgtg cgcgtgttgc gcgtcgccac cacgagctcg cccaggcgct gaagcgccac
     5641 cagtccgacg atgatggcga cccagttcat ccggcgtcga tgagctggaa cccggcagtg
     5701 aagcctgggc cgagcgcggt caccagcatc cggccggtgg cgccctggcg cagcatgcgc
     5761 tcgagcacga acatcaccgt cgccgccgac atgttgccgt agtcgcgcag cacgccgcgc
     5821 gcgtcggcga gcgccccttc cgccaggtga aacgcctcct cgagcgcggt caggaccttc
     5881 gcgccgccgg gatggcagac gaaatggcgg atgttctcga ggctcaatcc gtgccgtgcc
     5941 aggaagtcgc ccgcgacttg ccgcatatgg ccgcgcacca gcgccgggat gtcgcgcgag
     6001 aagagcgcct tcatgccgtc ctcaacgaca tcccagccca tgatgtcgag cgagttgggc
     6061 caggtgtgct cgccggcggg tccgagcgcg ggcccgtcgc cgcgacagga gagcaccgcc
     6121 gcggcggcgc cgtcgccgaa cagggccgtg gcgacgacat tgcttttcga ggtgtcgttc
     6181 ttgcggaagt tcagcgcgca gagctcgacc acggcgaaga gcacgcggct ggtcgggtgg
     6241 atgcgcgcga ggtcggcggc gcgcgcgagc ccgatcacgc cgccggcgca gccgaggccg
     6301 aagatcggca gtcgccgcac ctcgggcttc aggccgagac ggttgatgag catggcatcg
     6361 aggctgggcg tggcgacgcc cgtggtcgag gcgacgacca cggcgtcgat gtcgccgggc
     6421 gtcagccccg ccagcgcaag cgcatcgcgc gacgtctctt cgagcaagtt caccgcgttc
     6481 tcgaggaaga gggcgttccg gtcggtccag ccatgcggct tctcatacca gtcgatcggc
     6541 acgctcgaat agcgccgctc gatgccggca ttggcgaaca ccggcatgag ccggtcgagg
     6601 gcggcccccg cccgctcggc gaagaggtga cgggcgcgga tcgtgacctc gggctggccc
     6661 agcacatggg gcgggacggc cgtggaaagg gaggcgaggc gggggattgt cgtcgtcact
     6721 ggcgaagtgc tccatccgtc ggttaaactc cggccgctgc gtgcgattct ataacgagaa
     6781 cgagcgtccg ttcactaaat ttcggggcag ggatgttcga agggttcacg actgcgcgac
     6841 tggcgacggc cggcgcgaaa atccatctgc gccaaggcgg taagggacgg ccgctgctgt
     6901 tgctgcacgg ctatcccggg acgcatgtca tgtggcacaa aattgtcacg cgcttggcgc
     6961 gacgcttcac ggtggtcgcc agccccttgc tcgtgctgtg gggcgggaag ggggtggtcg
     7021 gccggctcta cggttcgctc gccctctggc gggagaaagc cgtcgacgtc acggggcggg
     7081 tggtgccttg cgaccatttt attcccgaag aggcgcccga ggaacccctc gccgcgcttg
     7141 cagcctttct cgcggggtga ccgatggagc
//
LOCUS       DASYNS010000011        12892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4215809, whole genome shotgun sequence.
ACCESSION   DASYNS010000011 DASYNS010000000
VERSION     DASYNS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 12892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12892
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4215809"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VGP50_00380"
     CDS             complement(<1..377)
                     /locus_tag="VGP50_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025847.1"
                     /translation="MPTQLVKARCLAASSLFLAALPVLTTVAMAAPCEGPGAPANTQT
                     RCLTAIAIPGNPLRAFDISFANPDRGEYYLADRSNAGVDIIDTVSLTFKRRLGGFVGV
                     AIVPGSVTPANPHGNTDNGVSGP"
     gene            675..1133
                     /locus_tag="VGP50_00385"
     CDS             675..1133
                     /locus_tag="VGP50_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8025848.1"
                     /translation="MSDAAAALTALILETFRLNGRFLAAGDALVASLGLTSARWQVLG
                     AVALSPVPLTTSQIARNMGLTRQSVQRLVNEMTQDGLLRLSANPQDRRAMLVARAARG
                     RSVYAASVERQEPWATRLAKSCRIEEIAAATAVLERLRSRLEEDSATRDE"
     gene            1153..1584
                     /locus_tag="VGP50_00390"
     CDS             1153..1584
                     /locus_tag="VGP50_00390"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HEV8025849.1"
                     /translation="MLAGQLALTIAAAFFGAAIYVNIAEQPARLTLDDAALLKEWKPS
                     YKRGFAMQAPLALFGCLFGVLAWRQTRDWPWLLGAVLIVANWPYTLLAIMPTNDVLMA
                     QDPMAAGPRSRALIQKWGRLHAGRSALGFSATLIFLWAALR"
     gene            1850..2707
                     /locus_tag="VGP50_00395"
     CDS             1850..2707
                     /locus_tag="VGP50_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025850.1"
                     /translation="MTWSRVYRRIVAALGLLVLGAGTAVAQAPLWLEPAYGDQPVLGP
                     RKAAGAVIWSHGRSIDSEDYEVETPPYIAVLRDSGWDAFRFNRQRAYDALGVSTRMLT
                     AEVHRLRQEGYRQVALAGQSFGAFLSLMSADVSDDVDAVIATAPAAYGSFSDFYDTWR
                     YNATRLYPLLQQLRHARVMVFYFHGDDFDPGGRGERSRDILLARKLPFVVVDQPASLA
                     SHWAAGGQQFADLFGRCIVEFLEAQQLGDDARCEDGTLVASPVEASRDRSPAMAATPS
                     PSVALAGSR"
     gene            2827..3303
                     /locus_tag="VGP50_00400"
     CDS             2827..3303
                     /locus_tag="VGP50_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV8025851.1"
                     /translation="MIKGLHHNAYRCRDSEETRRFYEGFLGLPLSGALRIKESMTGRR
                     TDVLHTFYRMDDGSFLAFFEAPDRPFEFKDQHDYDLHIALEVDPATLERMYAKGKAEG
                     REIRGVSDHGFIHSIYFRDPNGYVIELAAKQPNHAKAMDPKTNGARDILENWQAEK"
     gene            3607..4032
                     /locus_tag="VGP50_00405"
     CDS             3607..4032
                     /locus_tag="VGP50_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8025852.1"
                     /translation="MTDQPALTAPDRCPIARSLEFIGERWTILIIRDLNRFGPRKFQD
                     FEHGLAGIGPNTLSARLKKLEDAGIVERRFYEQHPPRAEYLLTAAGKELGPVLKALFE
                     WGRRHTVYTPATPEPDGRIKGGRKRLKAGRIGSGGDAAS"
     gene            4104..5282
                     /locus_tag="VGP50_00410"
     CDS             4104..5282
                     /locus_tag="VGP50_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV8025853.1"
                     /translation="MQRRIRWVAGTAVAAILIGAGAYAWLGVARDAGRSAGAAPPATR
                     PVPVTIGAVEAKEFAIYRVGIGTVQAYNTVTVKVRVDGEVQKIAFREGQDVNPGDLLA
                     VIDPRPLEAQLRQAQADKAHDEALLANAKLDLTRYSALATHDYATRQSVDTQNALVAQ
                     YRAAIEHDEAVIDNARVQLGYSTIVAPLAGRTGLRLIDQGNIVHAADAAGLVVITQIK
                     PIAVVFTLPQQDLPEITDALRSGSLEVLAYDQDNRLLLGKGQLELIDNRVEQSTGSVK
                     LKAVFPNDDERLWPGAFVNAWLRLAVRRGPVVPTAAVQAGPEGSYAFLVRPDASVELR
                     PVRVAAAWQGEALIEAGLAAGDRVVVDGQYKLRPGAHVIEAPKGGDKTAEAKPREATP
                     "
     gene            5279..8416
                     /locus_tag="VGP50_00415"
     CDS             5279..8416
                     /locus_tag="VGP50_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEV8025854.1"
                     /translation="MSLSTPFILRPIATSLLMAGLLMVGSVAYLLLPVAALPQVDYPT
                     IVVAAQLPGTSPETMASSVATPLERQLAQISGVSQLTSVSGIGNTAITIQFALSRNID
                     AAAQDVQAAINAAAGQLPKNLPSPPTYKKTNPSDAPIMLLALRSETLPITTVSDYADS
                     VVGQQISQIDGVALVTVAGFKPAVRVEANPVALAGRGLTLEDVRGAITSATLDGPKGT
                     LTGPRQSYTIENNDQLLKADGYRPLVVAWRNGSPVRLSDVAAVVDGPESILSSPTFDG
                     RPAVILVIQRQPGVNVIETVERIKGVLPRIKASIPPGIDIHIQSDRTQTIRASVNDVQ
                     FTLVLTAGLVVLVIFLFLRDFGATIIPGIAVPLSLIGTFAVMYLLDYSLDNLSLMALT
                     IATGFVVDDAIVMVENITRHLEGGKSPLQAALEGAREVGFTIISMSVSLIAVFIPVFL
                     MSGVIGRLFREFAVTVAIAIAVSAVVSLTLTPMMCAHLLRERTERRRGGLFRLSEAFF
                     DGMLAVYERGLGWVLRHRAITLAATMATVVLTGYLYVIIPKGFFPQQDTGFIVAVSEA
                     AQDISYPAMLERQARLIDIVRQDPGIDGVISIVGTNAINSTVNSGRMLINLKPRAERD
                     ASATEIINRLRPKLAPVEGIALFMQASQDINVGGRLTRTQYQYTLQDADLDELDRWAP
                     ILRRALEKLPELRDVATDQQIAGPTLALEINRDAAARLGVTTQAIDDTLYDAFGQRKI
                     AQYFTQLNSYWVVLEVDPAFQLDPNALDLIYVPKAGGGQVPLGEVVRPSATVRSLSVN
                     HQGQFPAITLSFNLAPGVALGQAVNAITRAKQEAGAPDTLLTTFQGNAQAFQSSLKSQ
                     PILVLAAIIAVYIVLGVLYESLVHPVTILSTIPSAGVGALLMLLAFHYELSVIAVIGI
                     ILLIGIVKKNAIMMIDFALEAERTQGLSPEQSIYRAAVLRFRPIMMTTMAALLGGVPL
                     ALGNGAGAEIRQPLGYAMVGGLLVSQFLTLYTTPVVYLALDRLRRQPSREPAAMRGMA
                     QAGVYARSAAASPAE"
     gene            complement(8554..8850)
                     /locus_tag="VGP50_00420"
     CDS             complement(8554..8850)
                     /locus_tag="VGP50_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025855.1"
                     /translation="MRKMKSPMRNILGLAGIGLALAAQPAPAQILGDADCPGGYSYNR
                     AYGICVPYGSTYVPNYYDYGNPYPPPFYGYGPYVVVTPPDRRRNPDRDRDRDRR"
     gene            complement(8885..11155)
                     /locus_tag="VGP50_00425"
     CDS             complement(8885..11155)
                     /locus_tag="VGP50_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV8025856.1"
                     /translation="MAWGRRILLLGTALSAAILAVFAAVCWSRAELVAPPASFMLYDR
                     HGRFLAQIGGGDAAGYGYWPAPPSSERMVRALLALEDRRFWEHPGVDPVAVVRAAAQN
                     VAAGRRISGASSIAMQVARLQHPASRTLLNKALEAGTALCLTLRYGHDAVLQHYLRLV
                     PFGNNSHGVAHAARFYFDKPVADLSWAEIALLAAIPQAPSRINPLTYDGHERAVARGR
                     RVLAYLRDVGVISPSDFILADRQIAAVALAQRPHRPSEAMHVVLRLERQLGGAEAWLA
                     EHQEARVIATLDLDLQRRIEALALKRLAEWRRAGAEQVAVAVLDRASREVLAWVGSAG
                     YFGPEAGAIDFGDTLRSPGSTLKPFLYALALDRGIIRADMNLRDDVDVAPGIENADRL
                     YLGRLLPRQALANSRNAAAAALVRSIGIGDTYQYLRGLGLHESAQPAEHYGLGLAVGA
                     LPTSLERLLRAYGALANDGTLEDLAWYRGEDRPAPQRVLSATAARQVSLFLADPMARL
                     PSFPRMGPAEFPFPVAVKTGTSQDYRDAWTMAYSPLFIVGVWVGRADAAPMQSLGGMG
                     SAAELAHDVILALHQETGHRLEDLPFPKPEAHRAVDACGGSGCPALFREWIPDETAQR
                     ADGVLIDRRTGTLATQSTPPGDVQEQPISMPVDGRLAATSTVADGVGIAIVSPESDAH
                     FLRDPELPAEAETIALRAAITGPFRDILWYVDDHPYGMTSGTAVRWTLAPGVHTFQAR
                     LMGRPESSRVIAITVD"
     gene            complement(11284..>12892)
                     /locus_tag="VGP50_00430"
     CDS             complement(11284..>12892)
                     /locus_tag="VGP50_00430"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HEV8025857.1"
                     /translation="RREIVDLATGAPLTLPAIAEPYRPGTLRRTLTVTAEPALLRLAA
                     GLDYLREYPFACTEQRVSHARAEIAAHRLHDLLLFEHTADRTEADYNATIAWIEGAID
                     GNDLVAYWPGTKGNVALTAWTVQFLTEAKAAGYAVNKALSDRLVGALARALRSDFPFF
                     LAGESLAERVWALEALAEAGVVDDGYAAELARRSQLMSLESQANVIETLSHSAQTDRA
                     VIGTLTSELWSGIVFRLVNGQEAYGGLQTDRLRGHPLLLPSEARTMARVIRASAAAGD
                     DPRRRLLVNALVASAGPTGWGSTNANAEAFLALTDFFATAAGGTGRGVSLVRGGEKRE
                     MTLDGGQPILRFTELHPEELAVMATGIGEPLGLLADTRYLPEADGSTVAAQAEGFVVA
                     EAIEKIAGGQPTAIDQPGRRLDFAVGDVVEQQLELVNPEERHQVAVVMPLAAGMEPLN
                     PNLATAPPEARPSQEPSLAPTYVAFLDDAVEYFYETLPKGTYRFAFRTRATVPGRFIA
                     PAAFAQMMYDDAVRGNGNGAEVVIERR"
BASE COUNT         2128 a         4350 c         4296 g         2118 t
ORIGIN      
        1 tccggccctg agacgccatt gtcggtgttg ccatgcggat tggccggcgt gacggagccc
       61 ggaacgatgg cgacgccgac gaagccgccg aggcgccgct tgaaggtcag gcttacggta
      121 tcgatgatat cgacgccggc attggagcgg tcggccaaat aatattcgcc ccggtcggga
      181 ttggcgaagc tgatatcgaa ggcgcgcaag ggattgcccg gaatcgctat cgcggtcagg
      241 catcgagtct gcgtgttggc cggcgcgccc gggccttcgc agggggccgc catcgcgacc
      301 gtggtcaaga ccgggagcgc cgcgaggaac agcgagctgg cggcgagaca tctcgccttt
      361 acaagttgcg taggcatagg tcctcctgat tgcagtcgta ttgagcggaa attggtgtgc
      421 tatgcgggcg ggagcctagg cgagccacgt gacagcagcg tgaaagaccg agccttgtca
      481 ctgcgatact gagccgagac gggagaagcg gcggccacaa gcatttgcgg cgaccatgac
      541 gtcgcctcgg tatcgggtgg ctgaccgcgg tcaccgacga ggtcggcacg accgccttcg
      601 tccgtgacac ctgttccgag cttttgcgaa gcggcgattg acaggatgct gacaataatg
      661 tcagtatgct gacaatgagc gacgcggccg cagcgctgac ggcactcatc ctggagacct
      721 tccgtctcaa cggccggttt ttggccgcgg gcgacgcgct ggtcgcgtcg ctcggactca
      781 cgagcgcgcg ctggcaggtg ctgggcgcgg ttgcgctctc gcccgtgccg ctgacgacct
      841 cgcagatcgc gcgcaatatg gggctcacgc gccaatcggt gcagcgcctc gtcaacgaga
      901 tgacgcagga tggcctgctg cgcctcagcg ccaatccgca ggatcgccgc gccatgctcg
      961 tcgcgcgcgc ggcccgtggc cgatccgtct atgcagcttc ggtcgagcgg caggagccct
     1021 gggctacgcg tctcgccaag agctgccgta tcgaggagat cgcggcggcg accgccgtgc
     1081 tcgagcggct gcgcagccgc ctcgaggagg attccgcgac gcgcgacgag tgacgagaaa
     1141 ggagatccgc tgatgctcgc aggacaactg gccttgacca tcgccgcggc gttcttcggt
     1201 gcggcgatct acgtcaacat cgccgagcag ccggcgcgcc tcactctcga cgatgcggcg
     1261 ctcttgaagg aatggaaacc gtcctacaag cgcggtttcg cgatgcaggc gccgctcgcc
     1321 ttgttcggct gcctgttcgg cgtgctcgcc tggcggcaga ccagggactg gccatggctg
     1381 ctgggcgcgg tgctcatcgt tgccaactgg ccttacacgc tgctggcgat catgcccacc
     1441 aacgacgtcc tgatggcgca ggacccgatg gcggccgggc cgcgaagccg ggcgctgatc
     1501 cagaaatggg ggcggctgca cgctggccgc tccgccctcg gcttcagcgc gaccttgatc
     1561 ttcttgtggg cggcgctgcg ctgaccgtac cggcgctcgt cgcgcctccc ccggttcacc
     1621 ccttcgggcg cggccggcgg gccggaatcg ataccatggg ttaagtcgcc gcacgcgatc
     1681 gattattcgt caccccgtga agggcctatt ccgcaatacg tcagggcggc agcgacggat
     1741 tgttgactgc ggttcaatag agaatgcagc ggatatcgga ttttccatcc tccagaccgt
     1801 gcctaagtga acctcggggc ctattggcac tctcgcgccg gaggcagcga tgacgtggag
     1861 cagggtgtat cgccgtatcg tggcggcctt gggcctgctg gtgctcggtg cagggaccgc
     1921 ggtggcgcag gcgcccttgt ggctcgagcc cgcttatggc gaccagcctg tgctcggccc
     1981 ccgcaaagcc gcgggtgccg tgatctggag ccatggccgc tcgatcgatt ccgaggatta
     2041 cgaagtggag acgccgccct atatcgcggt gctgcgcgat agcggttggg atgcgttccg
     2101 tttcaatcgc cagcgcgcct atgacgcgct tggcgtgagc acgcggatgc tcacggccga
     2161 ggtccatcgc ttgaggcagg agggctaccg ccaggtggct ctcgccggcc agtccttcgg
     2221 cgcgttcctc tcgctcatgt ccgccgacgt cagcgacgat gtcgacgcag tgatcgccac
     2281 ggcgccggca gcctatggca gcttcagcga cttctacgac acctggcgat acaacgcgac
     2341 caggctctat ccgctccttc agcagctccg ccatgcgcgc gtcatggtct tctatttcca
     2401 cggcgacgac ttcgacccgg gcggccgcgg cgagcgctcg cgcgacatcc tcctcgcgcg
     2461 caagcttccg tttgtcgtgg tggaccaacc ggccagcctc gcctcgcact gggcggcggg
     2521 cggtcagcaa ttcgccgatc tgttcgggcg ctgcattgtg gaattcctcg aggcgcagca
     2581 gctcggcgat gatgcgcgct gcgaagacgg gactcttgtc gcaagtccgg tcgaggcaag
     2641 ccgggatcgt tctccggcca tggccgcgac cccttccccc tccgtggccc tggccggcag
     2701 ccggtgaggc cgcccggtcc tctccctcaa tccagcgcgt gaaaagccgc ccgatcggcg
     2761 ctatctagaa cagatggctt gcctcgggcg gttcgcgatg cccgtcgcga cgagggaggt
     2821 gggagaatga tcaaaggcct gcatcacaac gcctatcgct gccgcgattc cgaggagacg
     2881 cggcgcttct atgaggggtt tctcggcctg ccgctctccg gcgcgttgcg catcaaggaa
     2941 tcgatgacgg ggcggcgcac cgacgtgctc cataccttct accggatgga cgacggctcc
     3001 ttcctcgctt tcttcgaggc gcccgatcgc cccttcgagt tcaaggacca gcacgactac
     3061 gacctgcaca tcgcgctcga ggtcgatccc gccacgctcg agcggatgta tgccaagggg
     3121 aaggccgaag gccgcgagat ccgcggcgtc tccgaccacg gcttcatcca ctcgatctat
     3181 ttccgcgatc ccaatggcta cgtcatcgag ttggccgcga agcagccgaa ccatgcgaaa
     3241 gccatggacc ccaagacgaa cggcgcccgc gacatcctcg agaactggca agccgagaag
     3301 tgacgttagc cctgtctcgc ggtgtttgct cctagcgcgt tccgagccgg cacggcaagc
     3361 ggagggccga tcagggggtc tgctggccgg gcccatccga cgtcagagag cggcgatatg
     3421 ggccgcgagc tctcgtggcg gcgtcttcgg ccgcccagtg accgccgttg agaacgctgc
     3481 cagatacgtt ctcggcatag tggcgccaga tcgaaacatg gctcccgtct ataactatcg
     3541 tcaggatagt tactttcaat aacagagtcg ctatcttttc aatagcgatt gggctatgtt
     3601 cgcccgatga ccgaccaacc ggccttgacc gcgcccgatc gctgcccgat cgcccgatcg
     3661 ctcgagttca tcggcgagcg ctggaccatc ctcatcattc gcgacctcaa ccgcttcggc
     3721 ccgcgcaagt ttcaggattt cgagcacggt ctggccggca tcggcccaaa cacgctgtcg
     3781 gcacggctga agaagctcga ggacgcaggc atcgtcgagc gtcgcttcta cgagcagcat
     3841 ccgccacgcg ccgagtatct cctgacggcg gcgggcaagg agctcggccc ggtgctcaaa
     3901 gctctcttcg agtgggggcg gcgccacacg gtttacacgc cggcgacgcc ggagccggat
     3961 ggcagaatca aaggcggccg gaagcgccta aaggcgggcc ggattggctc tgggggagat
     4021 gccgcttcgt gaacgtcaca tgggaggcgg ggctttgaca cgttaactcc agccgggcgc
     4081 agttaaactg ggagcattgc cacatgcaac gcaggattcg ctgggtagcc ggaaccgccg
     4141 tagcggccat cctcatcggc gcaggcgctt atgcctggct cggcgttgca cgggacgccg
     4201 ggcgctcggc gggcgcggcg ccgccagcga cgcgcccggt gccggtcacg atcggcgccg
     4261 tcgaggccaa ggaattcgcg atctaccgcg tcggcatcgg caccgtgcag gcctacaaca
     4321 ccgtcaccgt caaggtgcgg gtcgacggcg aagtgcagaa gatcgccttt cgcgaagggc
     4381 aggacgtgaa tccaggcgac ctgcttgcgg tcatcgaccc gcggccgctc gaagcgcagc
     4441 tgcgccaggc ccaggccgac aaggcgcatg acgaagcgct gctcgccaat gccaagctcg
     4501 atctcacgcg ctacagcgcg cttgcgacgc atgactacgc gacgcgacaa agcgtcgaca
     4561 cccagaacgc gctcgttgcg cagtatcgcg ccgcgatcga gcacgacgag gcggtcatcg
     4621 acaacgcacg cgttcagctc ggctacagca cgatcgtggc gccgctcgcc ggccgcaccg
     4681 ggctccggct gatcgaccag ggcaacatcg ttcacgccgc cgacgccgcg ggcctggtgg
     4741 tgatcactca gatcaagccg atcgccgtgg tcttcaccct gccgcagcag gatctgccgg
     4801 agatcaccga cgcgctgcgg agtggctcgc tcgaggtcct cgcctacgac caggataacc
     4861 gcctcctgct cggcaagggg cagctcgagc tcatcgacaa ccgcgtcgag cagagcaccg
     4921 gatcggtcaa gctgaaggcg gtttttccca acgacgacga gcggctatgg ccgggagcct
     4981 tcgtcaatgc ctggttgcgg ctcgcggtgc ggcggggccc ggtggtcccc accgccgccg
     5041 tgcaggctgg tcccgagggc agctacgcct tcctcgtccg gccggacgcg tcggtcgagc
     5101 tgcggccggt gcgcgtcgcc gccgcctggc aaggcgaagc gctgatcgag gcggggcttg
     5161 ccgcgggcga tcgcgtcgtg gtcgacgggc aatacaaact gcgccccggc gcgcatgtga
     5221 tcgaggcacc gaagggcggc gacaagacgg ccgaagcgaa accgcgggag gccacgccgt
     5281 gagcctctcg acacctttca tcctgcgtcc gatcgcgacc agcttgctca tggcgggcct
     5341 gctgatggtg ggctccgtcg cctatctgct gctgccggtt gccgcgctcc ctcaggtcga
     5401 ctaccccacg atcgtggtgg cggcgcagct gcccggaacc agccccgaga ccatggcgtc
     5461 ttcggtcgcg acgccgctcg agcgccagct cgcgcagatc tcgggcgtga gccagctcac
     5521 ctcggtgagc ggcatcggca ataccgcgat cacgatccag ttcgctctga gccgcaacat
     5581 cgacgccgcg gcgcaggatg tgcaggcggc gatcaatgcc gccgccgggc agctgcccaa
     5641 gaacctgccg agtccgccga cctacaagaa gaccaatcct tcggacgcgc ccatcatgct
     5701 gctggcgctt cgctcggaga cgctcccgat taccacggtc agcgactatg ctgatagcgt
     5761 cgtcggccag cagatctcgc aaatcgacgg cgtcgccctg gtgacggtcg cgggcttcaa
     5821 gccggcggtt cgcgtcgaag cgaatccggt cgcgctggcc ggtcgcggcc tgacgctcga
     5881 ggacgtgcgt ggcgccatta catcggcaac cttggacgga cccaagggca cgctgaccgg
     5941 gccgcgccag agctatacga tcgagaacaa cgatcagctc ctgaaagcgg acggctaccg
     6001 gccgctcgtc gtcgcctggc ggaacggctc gccggtgcgc ctttccgatg tagccgcggt
     6061 ggtcgacggc cccgagagca ttctatcgtc gcccaccttc gacggccggc cagccgtcat
     6121 cctggtgatc cagcgccaac ccggcgtcaa tgtcatcgaa acggtcgagc ggatcaaagg
     6181 cgtgctgccg cggatcaagg cgtccattcc gccgggcatc gacatccaca tccagagcga
     6241 tcgcacgcag acgatccgcg cctcggtcaa cgatgtgcag ttcaccctgg tgctcacggc
     6301 tggccttgtc gtcctcgtga ttttcctctt cctgcgcgat ttcggcgcca cgatcattcc
     6361 cggcatcgcc gtgccgctct cgctcatcgg cactttcgcc gtgatgtatc tcctcgatta
     6421 cagcctcgac aacttgtcgc tgatggcgct caccatcgcc accggcttcg tcgtcgacga
     6481 tgcgatcgtc atggtcgaga acatcacccg tcacctggag ggcggtaaat cgccgctcca
     6541 ggcggctctc gagggggcgc gcgaagtcgg gttcaccatc atctcgatga gcgtctcgct
     6601 gatcgcggtg ttcatcccgg tgttcctgat gagcggcgtg atcggacgcc tcttccgcga
     6661 gttcgccgtc accgtggcga tcgcgatcgc ggtctccgcc gtggtgtcgc tgacactgac
     6721 gcccatgatg tgcgcgcatc tgctgcgcga gcggacggaa cgccgccgcg gcgggctgtt
     6781 ccggctctcg gaggcattct tcgacggcat gctcgcagtt tacgagcgcg gcctcggctg
     6841 ggttttgcgc catcgcgcga tcacgctcgc cgcgacgatg gcgacggtcg tcctcaccgg
     6901 atatctctac gtcatcatcc cgaagggctt ctttccgcaa caggacaccg gcttcatcgt
     6961 cgccgtctcc gaggcggccc aagacatctc ctatcccgcc atgctggagc gacaggcgcg
     7021 gctcatcgac atcgtacgcc aggacccggg aatcgacggc gtgatctcga tcgtcggcac
     7081 caacgccatc aactccacgg tcaacagcgg ccggatgctc atcaacctga agccccgcgc
     7141 cgagcgcgac gccagcgcca cggagatcat caaccggctc cggccgaagc tcgcgcccgt
     7201 cgagggcatc gcgctcttca tgcaggcatc gcaagacatc aatgtcggcg ggcgcctcac
     7261 gcgcacgcaa tatcagtaca cgctgcaaga tgccgacctc gatgagctcg atcgctgggc
     7321 gcccattctg cggcgcgcgc tcgaaaagtt gccggagttg cgtgatgtgg ccaccgacca
     7381 gcagatcgcc ggcccgactt tggcgctcga gatcaatcgc gacgcggcgg cgcggctcgg
     7441 cgtcacgacg caagcgatcg acgacacgct ctacgatgcc ttcgggcagc gcaagatcgc
     7501 tcagtacttc acgcagctca acagctactg ggtcgtgctc gaggtcgatc cggcgttcca
     7561 gctcgacccg aacgcgcttg acctgatcta tgtgcccaag gccggcggcg gccaggtgcc
     7621 gctcggcgag gtagtgcggc cgagcgcgac ggttcggtcg ctctcggtca atcaccaggg
     7681 acagttcccg gcgatcacgc tgtccttcaa cctcgcgccg ggcgtggcgc tcggccaggc
     7741 ggtcaacgcg atcacgcgcg cgaagcagga agcgggcgca cccgatacgc tgctcacgac
     7801 cttccagggc aacgcccagg ccttccagtc ctcgctcaag agccagccca tcctggtgct
     7861 ggccgcgatc atcgccgtct acatcgttct gggcgtcctc tacgagagcc tggtgcatcc
     7921 cgtgacgatc ctctcgacca tcccctcggc gggcgtgggc gcgctcctga tgctgctcgc
     7981 cttccattac gagctcagcg tcatcgccgt gatcggcatc atcctgctca tcggcatcgt
     8041 caagaagaac gcgatcatga tgatcgactt cgccctcgag gcggagcgca cgcagggcct
     8101 gagccctgag cagtcgatct atcgcgcggc agtgctgcgt ttccggccga tcatgatgac
     8161 gaccatggcg gcgctgctcg gcggtgtgcc gctcgcgctc ggcaacggcg ccggcgccga
     8221 gatccgccag ccccttggct acgccatggt cggcggcctc ctggtctcac aattcctgac
     8281 gctctacacg acgccggtcg tgtatctcgc gctcgaccgc ctgcggcgcc agccgtcgcg
     8341 cgagcccgcg gcgatgcgcg gcatggccca ggcaggcgtc tatgcgcgct ccgccgcggc
     8401 gagcccggcc gagtgagccc acgcggcgga gcctcttgga tcgcattgcc gcgaagcgct
     8461 cggaccgtcc gctgacggcg acgggcaagg agctcggccc ggtgctcaaa gctctggtcg
     8521 cgtcggggcg gcgtcacacg gtttgcgcgc ccgtcagcgg cgatcgcggt cgcgatcgcg
     8581 gtcagggtta cggcggcggt cgggcggcgt gacgacgacg tagggcccgt atccgtagaa
     8641 gggcggcgga taggggttgc cgtagtcgta gtagttgggc acgtaggtcg agccataggg
     8701 cacgcagatg ccgtaggctc gattatagga atagccgccc ggacaatcgg catcgccgag
     8761 gatctgcgcc ggcgccggct gcgccgccaa tgccagtccg atgccggcga ggccgagaat
     8821 gttccgcatc ggcgacttca tctttcgcac ctgacctccc tgcaacggtc gggaagcgga
     8881 ggcgtcaatc gacagtaatg gcgatgacgc gcgagctctc cgggcgaccc ataagtcgtg
     8941 cctggaatgt atggacgccc ggcgcgagtg tccagcggac cgccgtgccg ctcgtcatgc
     9001 catagggatg atcgtccaca taccatagga tatcccggaa cggcccggtg atggcggcgc
     9061 gcagcgcaat ggtctcggcc tctgcgggca gctccggatc cctgaggaaa tgcgcgtcgc
     9121 tttccggtga cacgatagca atgccgacac catcggccac ggtgctcgtg gcggccagcc
     9181 gtccgtcgac gggcatcgag atcggctgct cctgaacatc ccccggcggc gtcgactgcg
     9241 tcgccagcgt tccggtccgc ctgtcgatca gcacgccatc ggcacgctgc gccgtctcgt
     9301 cgggtatcca ttcgcggaaa agcgcggggc aaccgctgcc gccgcaggcg tcgacggcgc
     9361 ggtgggcttc gggcttgggg aagggcaggt cctcgagacg atggccggtc tcctggtgga
     9421 gcgccaggat cacgtcatgc gccaactcgg ccgcgcttcc catgccgccc agcgactgca
     9481 tcggtgcggc gtcggcgcgt cccacccaga caccgacgat gaagagcggc gaataggcca
     9541 tggtccaggc gtcgcgataa tcctgtgacg tgccggtctt gaccgcgacg ggaaagggga
     9601 attccgccgg gcccatgcgc gggaagctcg gcagccgggc catgggatcg gcgaggaaca
     9661 gcgagacctg ccgtgccgcc gtcgccgaca gaacacgctg cggcgcgggc cggtcctcgc
     9721 cgcggtacca ggcgaggtct tcgagcgtgc catcattggc gagcgccccg taagcgcgca
     9781 gcaaccgctc gagcgaggtc ggaagcgcgc cgacggcgag gccgaggcca taatgctctg
     9841 ccggctgcgc gctttcgtga agcccgaggc cgcggagata ttgatacgtg tcgccgatgc
     9901 cgatgctacg caccagcgcc gcggcggcgg cattgcgcga attggcgagc gcctggcgcg
     9961 gcagcaatcg cccgagatag aggcggtcgg cgttctcgat tcccggcgcg acatcgacgt
    10021 cgtcgcgcaa gttcatatcg gcgcggatga tgccgcggtc gagcgccagc gcgtagagga
    10081 acggtttcag ggtgctgccg ggcgagcgca gcgtgtcgcc gaaatcgatg gcgcccgcct
    10141 ccgggccgaa gtagccggcc gagccgaccc aggccagcac ctcgcgggac gcgcggtcga
    10201 gaaccgcgac cgccacctgc tccgcgcccg cgcgccgcca ttcggcgagg cgcttcaagg
    10261 cgagcgcctc gatccgccgc tgcagatcga ggtcgagcgt cgcgatgaca cgcgcctcct
    10321 gatgctcggc gagccaagcc tcggcgccgc cgagctggcg ctcgaggcgg agcaccacat
    10381 gcatcgcttc gctcggccga tgcgggcgct gagccagcgc gacggcagcg atctgccggt
    10441 cggcgaggat gaaatcgctc ggcgagatga cgccgacatc gcgcagatag gcgagaacgc
    10501 gccggccgcg cgcgacggcc cgctcatggc cgtcataagt cagcggattg atgcggctcg
    10561 gcgcttgcgg aatggcggcg agaagcgcga tctcggccca actcagatcg gcgacgggct
    10621 tatcgaaata aaagcgcgcg gcatgggcga cgccatggct gttgttgccg aagggcacga
    10681 gccgcagata gtgttgcagt actgcatcgt gcccatagcg cagcgtgaga caaagcgccg
    10741 tgccggcctc cagcgccttg ttgaggaggg tgcgcgacgc cggatgctgg agccgcgcca
    10801 cctgcattgc gatcgaggac gcgccagaga tccggcgtcc cgccgcgaca ttctgcgcgg
    10861 ccgcgcgcac gaccgcgacc gggtcgacgc ctggatgttc ccagaagcgc cgatcctcga
    10921 gcgcgagcag cgcccgcacc atccgctccg acgagggcgg tgccggccaa tagccatacc
    10981 ccgccgcatc gccgccgccg atctgcgcca gaaagcgccc atgccggtca tagagcatga
    11041 agctcgccgg cggtgcgacc agctccgccc gcgaccaaca gaccgccgcg aacaccgcaa
    11101 gtatcgcggc ggaaagcgcg gtgccgagga ggaggatgcg acggccccat gccatcagcg
    11161 gcgctcccga acgctctttt ccaaccgcga tgtaactacc tccccctcgt cgagggagga
    11221 gaagatactt gctctggatt cgcgcgctag cgcgaacggt gagatgatct ttcgtcccat
    11281 gctttaccgc cgctcgatca cgacctcggc gccgttgccg ttgccgcgga cggcgtcgtc
    11341 gtacatcatc tgcgcaaagg ccgcgggcgc gatgaagcga ccgggcacgg tggcgcgcgt
    11401 gcggaaggcg aagcgatagg tgcccttcgg cagcgtctcg tagaaatatt cgacggcgtc
    11461 gtcgaggaag gcgacatagg tcggcgccaa gctcggctct tgcgacggcc gcgcttcggg
    11521 cggcgccgtc gcgagattgg ggttgagcgg ctccatgccg gcggcgagcg gcatgacgac
    11581 cgcgacctga tgccgctctt ccggattcac cagctcgagc tgctgctcca cgacgtcgcc
    11641 caccgcgaaa tcgaggcgcc tgcccggctg atcgatcgcc gtcggctgtc cgccggcgat
    11701 cttctcgatc gcctcggcca cgacgaagcc ttctgcctgc gccgcaaccg tgctgccatc
    11761 ggcctccggc agatagcgcg tgtcggcgag aagtccaagc ggttcgccaa tgccggtggc
    11821 cattaccgcc agctcttcgg gatggagctc ggtgaagcgc agtatcggct gtccgccgtc
    11881 gagcgtcatc tcgcgcttct cgccgccgcg caccaggctt acgccgcgtc ccgtgccacc
    11941 cgcagccgtg gcgaagaaat cggtgagcgc cagaaacgct tcggcattgg cattggtgct
    12001 gccccagccg gtgggcccgg cgctggcgac gagagcattc accagaaggc gtcggcgcgg
    12061 atcgtctccg gcggcggcgc tggcgcggat cacgcgcgcc atggtccgcg cctccgacgg
    12121 cagcagcagc ggatgcccgc gcagccgatc cgtttgcagt ccgccatagg cttcttgccc
    12181 attgacgagc cggaagacga tacccgacca gagctcgctg gtcagagtcc ctatcaccgc
    12241 ccggtcggtt tgcgcgctgt gcgacaaggt ctcgatcaca ttcgcctggc tttcgagcga
    12301 catgagctgc gaccgccgcg ccagctccgc cgcatagccg tcatcgacca cgcccgcttc
    12361 ggcgagcgct tcgagcgccc agacgcgctc cgccaggctt tcgcccgcga ggaagaaagg
    12421 aaaatccgag cgcaaggcgc gggcgagcgc accgacgagg cgatcggaga gcgctttgtt
    12481 gacggcgtag ccggcggcct tcgcttcggt gaggaactgg accgtccagg cagtgagagc
    12541 gacgttgccc tttgtgccgg gccagtaggc cacgaggtcg ttgccgtcga tcgcgccctc
    12601 gatccaggcg atggtcgcgt tgtagtccgc ctcggtgcga tccgccgtat gctcgaacag
    12661 caggaggtca tggaggcggt gtgccgcgat ctcggcccgc gcatggctga cgcgctgctc
    12721 ggtgcaagcg aagggatatt ccctgagata atcgaggccg gcggcaaggc ggagcagcgc
    12781 cggctcggcg gtgacggtga gggtccgcct cagcgtgccg ggccgatagg gctcggcaat
    12841 cgcaggcaag gtcagcggtg ccccggtcgc gaggtcgacg atctcgcgcc gg
//
LOCUS       DASYNS010000012         4527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1095416, whole genome shotgun sequence.
ACCESSION   DASYNS010000012 DASYNS010000000
VERSION     DASYNS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095416"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VGP50_00435"
     CDS             complement(<1..334)
                     /locus_tag="VGP50_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025858.1"
                     /translation="MAAARKRHAKPAVERAPAPLGWLSDDALEIERRHWRGKTEIGAI
                     ETLEPGQPFFGTFRVSSAAGSGYDVEIRSLDRRSNSCGCADHRVNGLGTCKHIEGALA
                     RLRRRGKRA"
     gene            complement(665..2677)
                     /locus_tag="VGP50_00440"
     CDS             complement(665..2677)
                     /locus_tag="VGP50_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV8025859.1"
                     /translation="MDVVTVSGQSAGLRVVAYPGEHKILLAMSLDDGKLDTQTQNLAG
                     FAIWRKPSGAAEQILSNRIAFTEGVSNETTAATRQWYPSDQAPFQKFRWVDVPPDGFD
                     APITYRIKALYFAGQGMTLKDGPEVSVTVAPAEPRYASFHPAFTRGYIASQAYADKFH
                     NADIRPKGAKTSDFDTKPYQEQYAWLGAGARAALFDFLADCEKDTSARIDVFAYDLDE
                     PDVIASICRMGKQGRLRAVLDNAPLHVPGKSGQAPPEVKAAAMIKAAAGDANVKQGHF
                     DRFQHNKVFIKRDAGGKAERVLFGSMNFSVRGIYVQANNIVVVDDANVAGMFASAFDE
                     AFTDGVKATPFRKNPIAKGYMVGSPGGTAQLPKFSLALSPHADNTVSLGPMADRIRKA
                     TSSVLFAVMAPTGGGPVLQSLRTIAAKPTVFSYGTVETDKGLAVQSPDGAMGDLTGFA
                     ILQKNVPAPFQQEFSGGPGMHIHDKLVVVDFNGQNPTVFTGSSNLAAGGETANGDSLA
                     MIEDADVAGMYAIEAVAMFDHYHFRKAMQQATKPQPLTLWYPGKANAPLPWWKPYYDP
                     TKIQLRDRCLFAGAPLPAGVATTKNVDWSAVDAPGAAAAKTTGAKAATKPATKRAATR
                     GKAGRKRPPAKKVSPKRRPAAKKAKRKAAAPRKAKRKAVPSRKRRQ"
     gene            2863..3135
                     /locus_tag="VGP50_00445"
     CDS             2863..3135
                     /locus_tag="VGP50_00445"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEV8025860.1"
                     /translation="MCRNIKTLFNFEPPATEEEIRASALQFVRKLSGFNKPSQANEAA
                     FNAAVESVADAARVLVASLATTAAPRDRQVEIEKARERSRLRFGRA"
     gene            complement(3147..>4527)
                     /locus_tag="VGP50_00450"
     CDS             complement(3147..>4527)
                     /locus_tag="VGP50_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV8025861.1"
                     /translation="LFIGQVARDTQEREAFQEVDYRRMLGPLAKWVAQIDDARRIPEF
                     VSRAFHVATSGRPGPVVLALPEDMLTDEVETADAQPYAAVETWPGAAPMAAFRAALAA
                     AKRPFLLLGGSAWDGEAVALIQDFAAANKLPVGCVFRRQDRFDNTHPCYAGDVGLGVN
                     PKLAERVKASDLLIALGARLGEATTGGYTLLAIPTPKQRLVHIHPDPEELGRIYQPAL
                     AINATPRAFARAAAALAPLSDLPWAAATAAAHEDYLAWRKAPPSPGAIQLGEIMLWLD
                     HELPADAIVTNGAGNFATWVHRFHQYRRFGTQLAPTSGSMGYGFPAALAAKLRHTDRV
                     VVCFAGDGDFLMTGQELATAVQQRLGVIVLLVNNNMYGTIRAHQERHYPERVIATALH
                     NPDFAALARAYGADGAVVERTADFAPAFARARAATGPFIIELRVDPEALTIRQSLSEI
                     RAQALASRK"
BASE COUNT          711 a         1474 c         1573 g          769 t
ORIGIN      
        1 aggcgcgctt gccgcgccgg cgcaaccgcg ccagcgcgcc ctcgatgtgc ttgcaagtgc
       61 cgagcccgtt gacgcggtgg tcggcgcagc cgcaggaatt gctccggcgg tcgagcgagc
      121 ggatctcgac atcgtagccg ctaccggccg ctgaacttac gcggaaggtg ccgaagaagg
      181 gctgcccggg ctccagcgtc tcgattgcgc cgatctcggt cttgccgcgc cagtgccgtc
      241 gctcgatctc cagggcatca tcggagagcc agccgagggg cgccggcgcg cgctcgaccg
      301 ccggcttcgc gtgacgctta cgcgcggctg ccaacctcac gtcattccgg ccattcgatg
      361 ctctaagctg ccgcgaatcg agcatagtcc ccctcgacac cttaaccgtg cgcccacaac
      421 atattggacg aagcttgacg ccacgcgcaa catatcgatg ggaatcgcga tcgggacctt
      481 tgctcgatga cgcgcccgag ccgttcggcc gccggcgata ttcggtgccg ccacgcgcct
      541 cggccgcgct gctgccggaa tctagcggcg actcgcggtg gctcggtttc cgacgacgtc
      601 gggcggtcgc gtgagaaggt gctgggcgcg gtggtgcgcc tccgctcagc ggagcctgga
      661 gcgattactg gcggcgcttg cgggacggca cggctttgcg cttggccttt cgcggtgcgg
      721 cggccttgcg cttggccttc ttcgccgcag gcctgcgctt gggcgacacc ttcttcgctg
      781 gcggacgttt gcgccctgcc ttgccgcggg ttgcggcccg tttcgtcgcc ggcttcgtcg
      841 cagccttggc gcccgtcgtc ttggcggcgg cggcgcctgg ggcatcgact gcgctccaat
      901 cgacgttctt cgtcgtggca acgcctgccg gcagcggcgc gccggcaaag aggcagcgat
      961 cccgcaactg gatcttggtc ggatcgtaat agggcttcca ccagggcagc ggcgcgttgg
     1021 ccttgccggg gtaccagagc gtcagcggct gcggcttcgt cgcctgctgc atcgccttgc
     1081 ggaagtggta gtgatcgaac attgcgaccg cctcgatcgc gtacatgccg gcgacgtctg
     1141 cgtcctcgat catggcgagg ctatcgccat tggccgtctc gccaccagcc gcgagattcg
     1201 acgatcccgt gaagacggtg ggattctggc cgttgaagtc gacgacgacc aacttgtcgt
     1261 ggatatgcat gcccggaccg ccgctgaact cctgctggaa cggcgcgggt acgttcttct
     1321 gcaagatggc gaatcccgtg aggtcgccca tggcgccgtc gggactctgc acggcgagac
     1381 ctttgtcggt ctcgacggtg ccgtaggaga agaccgtcgg cttcgccgcg atggtgcgca
     1441 gactttggag caccggccct ccgcccgtgg gcgccatgac cgcgaagagg acggagctcg
     1501 tcgccttgcg gatgcgatcg gccatgggtc cgagcgagac agtgttgtcg gcgtgcggtg
     1561 acagcgccag cgagaatttc ggcaattgcg ccgtgccgcc cggcgagccg accatgtatc
     1621 ctttcgcgat cggattcttg cgaaacgggg tcgctttcac gccatccgtg aacgcctcgt
     1681 cgaaggcgct cgcgaacata cccgcgacat tcgcgtcgtc gacgacgacg atgttgttcg
     1741 cctggacata gatgccgcgc accgagaagt tcatcgagcc gaaaagcacg cgctccgcct
     1801 tgccgccggc atcgcgcttg atgaacacct tgttgtgctg gaagcggtcg aaatggcctt
     1861 gcttcacatt ggcgtcgccg gcggcggcct tgatcatcgc cgccgccttg acctcgggcg
     1921 gcgcctggcc gcttttgccc ggcacatgga gcggcgcatt gtcgagcacc gcgcgcagcc
     1981 gcccctgctt gcccatgcgg cagatcgaag cgatgacgtc gggctcgtcc aggtcgtagg
     2041 cgaagacatc gatcctggcg ctcgtgtcct tctcgcaatc ggcgaggaaa tcgaagagcg
     2101 cggcgcgcgc gcccgcgccc agccacgcgt attgctcttg atagggcttg gtatcgaagt
     2161 ccgacgtctt cgcacccttg gggcggatat cggcattgtg aaacttgtcg gcataggctt
     2221 gcgatgcgat gtagccgcgc gtgaaggccg ggtgaaagct ggcgtagcgc ggttcggcgg
     2281 gagccaccgt gaccgacact tcgggcccgt ctttcagggt catgccttgg ccggcgaagt
     2341 agagcgcctt gatgcggtag gtgatcggcg cgtcgaagcc gtcgggcggg acatcgaccc
     2401 agcggaactt ctgaaacggc gcctggtccg acggatacca ctggcgcgtc gccgccgtcg
     2461 tctcgttcga gacgccttcg gtgaaggcga tccggttgga cagaatctgc tcggcggcgc
     2521 ccgagggttt gcgccagatg gcgaagcctg cgagattctg cgtctgagtg tcgagcttgc
     2581 cgtcatcgag cgacatcgcc agcagaatct tgtgctcgcc cggatacgca acgacccgca
     2641 ggccggcgct ttggccggaa accgtgacaa cgtccatgat gccccccgca cggaaaagcc
     2701 gcgcaagacg tcggtgcgcg gcgaagcacg gactctaggt cgatcgggta gtgcaattca
     2761 atcgtcattc cccggacgat tgtgaagtat ttgtggcggg cgcgtcgcgc tggacaaccg
     2821 cctgcgccag cgcaacatca agcgccgcat cgggaggtcg tcatgtgccg caacatcaag
     2881 acgctgttca actttgagcc gcccgcgacc gaggaggaga tccgcgcctc tgcgctgcaa
     2941 ttcgtgcgca agctcagcgg cttcaacaag ccgtcgcagg cgaatgaggc agccttcaac
     3001 gcggccgtcg agagcgtcgc ggacgcggcg cgggtgctgg tcgcgtcact ggcgacaacg
     3061 gccgctccgc gcgatcgcca ggtggagatc gagaaagcgc gagagcgctc gcggctgcgc
     3121 ttcggtcgtg cctgacaagg cgggcctcac ttccgggaag caagcgcctg ggcgcggatc
     3181 tcgctcagtg actggcggat ggtgagcgct tcggggtcga cgcgcagctc gatgatgaaa
     3241 gggccggtcg cggcgcgcgc gcgcgcgaag gccggcgcaa aatccgccgt gcgctcgacg
     3301 acggcgccgt ccgcgccata ggcgcgggcg agcgcggcga agtctggatt gtgcagcgcc
     3361 gtggcgatga cgcgctcggg ataatggcgc tcctgatgcg cgcggatcgt gccatacatg
     3421 ttgttgttga cgaggagcac gatgacgccg agccgctgct gcaccgccgt cgccagctcc
     3481 tgccccgtca tcaggaaatc gccgtcaccg gcgaaacaga cgacaacgcg gtcggtgtgc
     3541 cgcagcttcg ccgccagggc cgccgggaag ccgtagccca tcgagccgct ggtcggtgcc
     3601 agctgcgtgc cgaagcgacg atactgatgg aagcggtgga cccaggtggc gaagttgccg
     3661 gcgccgttgg tgacgatcgc atcggccggc agctcgtgat cgagccacag catgatctcg
     3721 ccgagctgga tcgcgccggg cgacggcggg gctttgcgcc aggcgagata atcctcatgc
     3781 gccgccgccg tcgcggcggc ccagggcagg tcggagagcg gcgcgagagc agcggccgcg
     3841 cgcgcgaagg cgcggggcgt ggcgttgatc gccagcgccg gctgatagat gcggccgagc
     3901 tcctcgggat cgggatggat gtggacgaga cgctgctttg gagtcggtat ggcgagaagc
     3961 gtgtagccgc ccgttgtcgc ctcgccgagc cgagccccca gcgcgatgag aagatcgctc
     4021 gccttgacgc gctcggcgag cttcggattg acgccgagac cgacatcgcc ggcgtaacac
     4081 ggatgggtgt tgtcgaaacg gtcctgacgg cggaagacgc agccgaccgg cagcttattc
     4141 gccgccgcga agtcctgaat gagcgccacc gcctcgccat cccaagcgct gccgcccagc
     4201 agcaggaagg gccgcttcgc cgccgccagc gccgcgcgga aggccgccat cggcgcagcg
     4261 cccggccaag tctcgaccgc tgcatagggt tgcgcgtcgg cggtctccac ttcgtcggtg
     4321 agcatgtctt cgggcagcgc cagcaccacc ggacccgggc ggccgctcgt cgccacatgg
     4381 aaggcgcggc tcacgaattc ggggatgcgc cgggcgtcgt cgatctgcgc cacccatttg
     4441 gcgagcgggc cgagcatgcg gcgataatcc acctcctgga aggcctcgcg ctcctgagtg
     4501 tcgcgcgcca cctggccgat gaagagg
//
LOCUS       DASYNS010000013         4372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2187671, whole genome shotgun sequence.
ACCESSION   DASYNS010000013 DASYNS010000000
VERSION     DASYNS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2187671"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..2822
                     /locus_tag="VGP50_00455"
     CDS             87..2822
                     /locus_tag="VGP50_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEV8025862.1"
                     /translation="MAFSDRSERDQRPSPDALLAQAVQEGRGRLKIFLGAAPGVGKTY
                     EMLQSAQARRQEGIDLVIGVVETHKRRETEALLAGLEVVPRRRIEYKGRTLDEMDLDA
                     ILLRRPKLVLVDELAHTNAPGSRHPKRYLDVEELLGAGIDVYTTLNIQHVESLNDVVA
                     QITRIRVRETVPDSILDRADDIEVIDLSPADLIQRLKEGKVYLPQQAERAIRHYFMPG
                     NLTALRELALRRTAQRVDEQMLTYMRAHAISGPWAAGERLLICVSEDPAGAGLVRYAK
                     RVADRLHAPWTALYVETARYHRLDEKERDRIADCLRLAEKLGGAAITIPGQRIAEEVV
                     SYAKSNNITHIVIGKSERSRWFEMLHGSVVHDLVRDAGSISVHVIASDPEDAVPAKTV
                     QTEQPPEIFDPMPYVATLGIIAGALAIGLPLQKFLNVANIALVFLTAVFISAIRFGLL
                     PSLFAAVVSVLIYNFFFLPPLYTFTIADPENVVALFFFLIVAVLASNLASRTHNQVIT
                     TRSRARTTEELFAFSRKLAGIGKLDDLLWVTVYQIAAMLKVRVVLLLPEGNGLAVRAG
                     YPPEDQLDDADLAAARWSWDHNRAAGRGADTLPGARRLFLPLRTGRGPVGVLGIDSDK
                     PGPLLTPDGRRLLDALADQGAIAMERITLAEDVDRTRVMAETERLRAALLTSISHDLR
                     TPLAAIIGSLTSLRGYGKTYDEETRDELLTTVQAEAERLNRFIGNLLDMTRLESGAVE
                     IKPEVVDIGEIVGTALERTAKMLADHKVEVDVDPDLPMLRLDYLLFEQVLFNLLDNAA
                     KYAPPGSLVRLSAHREGVAVVVEVIDEGPGIPPADLERIFDKFYRVYAQDRQRAGTGL
                     GLAIGRGFIEALGGTIIAGNRADRSGAIFTIRLPIRGAERVEQRQEGAWLDAPPS"
     gene            2798..3490
                     /locus_tag="VGP50_00460"
     CDS             2798..3490
                     /locus_tag="VGP50_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8025863.1"
                     /translation="MARRTTVLIVDDEPPIRRFLRTSLAAQDYEVIEAGNGAEAIRAM
                     ASAAPDLVILDLGLPDMNGTEVIRSIRGSSPVPIIVLSVRNDERGKVEALDLGADDYI
                     TKPFGMEELVARIRTALRHRFQEKGETPVFVAGDLSVDLVRRIVKRSGAEVKLSPKEY
                     DLLRLLVENAGKVLTHRHLLAKVWGPAHTEDAQYLRVFIRSLRHKLEADPARPALILT
                     EPGVGYRLASAL"
     gene            complement(3585..3998)
                     /locus_tag="VGP50_00465"
     CDS             complement(3585..3998)
                     /locus_tag="VGP50_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV8025864.1"
                     /translation="MFISRRRLRVDWGHCDPAGIVFYPNYFRWFDECTSTLFESAGLP
                     LPQLYRTHGLKGFPLLEAGAKFAAAASFGDDLDSEAGVSEWSAKTFKVQHRFLRGATL
                     ILEGWELRICAIPHPDDPGRMKAAPIPDEVKRRLG"
     gene            3991..4179
                     /locus_tag="VGP50_00470"
     CDS             3991..4179
                     /locus_tag="VGP50_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025865.1"
                     /translation="MNMVLARLSAADYGRNRDRGERAVVPQRQDPQLVAIGRIARTGV
                     CDQSFRANELTHLPRRHL"
BASE COUNT          706 a         1573 c         1363 g          730 t
ORIGIN      
        1 cccggcgctt cgcgccgggc ccctccctct cccacgaggg gagaggggat tcacgctttc
       61 agccgcgagc gcattagaat acccccatgg ccttctccga ccgatccgag cgtgaccagc
      121 ggccgtcgcc cgatgctttg ctcgcgcaag cggtgcagga ggggcggggg cggctcaaga
      181 tcttcctcgg cgcggcgccg ggcgtcggca agacctatga gatgctgcaa tcggcgcagg
      241 cccggcgcca ggagggcatc gatctcgtca tcggcgtcgt cgagacccat aagcgccgcg
      301 agacggaagc gctgctcgcc ggcctcgagg tcgttccacg ccggcgcatc gagtataagg
      361 gccggaccct cgacgagatg gatctcgacg ccatcctgct gcggcggccg aagctcgttc
      421 tcgtcgacga gctcgcccac accaacgcgc caggaagccg ccatcccaag cgctatctcg
      481 acgtcgagga gctgctcggc gccggcatcg acgtctacac cacgctcaac atccagcatg
      541 tcgagagcct gaacgacgtt gtcgcgcaga tcacccgcat ccgcgtgcgc gagacggtgc
      601 ccgacagcat cctcgaccgc gccgacgaca tcgaggtcat cgacctctcg cccgcggacc
      661 tgatccagcg cctgaaagag ggcaaggtct atctgccgca gcaggccgag cgcgccatcc
      721 gccactactt catgccgggc aatttgacgg cgttgcgcga gctcgcgctc cgccgcacgg
      781 cgcagcgcgt cgacgagcag atgctcacct atatgcgcgc ccatgccatc agcggcccct
      841 gggccgcggg cgagcgcctg ctgatctgcg tcagcgagga tccggccggc gccggcctcg
      901 tgcgctacgc caagcgcgtc gccgaccgtc tccatgcgcc ctggaccgcg ctctatgtcg
      961 agaccgcgcg ctatcaccgc ctcgacgaga aagagcgcga ccgcatcgcc gattgcctgc
     1021 gcctcgccga gaagctcggc ggcgcggcca tcaccatccc cgggcagcgc atcgccgagg
     1081 aggtcgtgag ctacgccaag tccaacaaca tcacccacat cgtcatcggc aagtcggagc
     1141 gctcgcgctg gttcgagatg ctgcacggct ccgtggtgca tgatctcgtg cgcgacgccg
     1201 gctcgatcag cgtccatgtc atcgccagcg atcccgagga tgcggtgccg gcgaagacgg
     1261 tgcagaccga gcagccgccg gagatcttcg atcccatgcc ttatgtggcg acgctcggca
     1321 tcattgccgg cgcgctcgcc atcggcctac cgctgcagaa attcctcaac gtcgccaaca
     1381 tcgcgctggt cttcctcacg gccgtgttca tctcggcgat ccgcttcggc cttcttccct
     1441 cgctcttcgc cgcggtcgtc agcgtcctca tctacaactt cttcttcctg ccgccgctct
     1501 ataccttcac catcgccgat cccgagaacg tcgtcgcgct gttcttcttc ctgatcgtcg
     1561 ccgtgctcgc cagcaatctg gcgtcgcgta cgcacaacca ggtgatcacc acgcggagcc
     1621 gcgcgcgcac caccgaggag ctcttcgcct tcagccgcaa gctcgccggc atcggcaagc
     1681 tcgacgacct gctttgggtc accgtctatc agatcgcggc tatgctcaag gtgcgcgtgg
     1741 tgctgctgct gcccgagggg aacggccttg ccgtgcgcgc gggctatccg ccggaggacc
     1801 aactcgacga cgccgatctc gccgcggcgc gctggagctg ggaccacaac cgcgccgccg
     1861 gccgcggtgc cgacacgctc cccggcgcgc ggcgcctctt cctgccgctc cgcaccgggc
     1921 gcgggccggt gggcgttctt ggcatcgaca gcgacaagcc cgggccgctc ctgacgcccg
     1981 acggccgccg gctcctcgac gctctcgctg accagggcgc gatcgccatg gagcgcatta
     2041 cgcttgccga ggacgtcgac cgcacgcgcg tcatggcgga gaccgagcgt ttgcgcgcgg
     2101 cgctgctgac ctcgatctcg catgatctgc gcacgcccct ggcggcgatc atcggctctc
     2161 tcaccagcct gcgcggctat ggcaagacct atgacgaaga gactcgcgac gagctgctga
     2221 cgacggtcca ggccgaggcc gagcggctca accgcttcat cggcaatctc ctcgacatga
     2281 cgcgcctcga atcgggcgcc gtcgagatca agcccgaggt ggtcgatatc ggcgagatcg
     2341 tcggcaccgc gctcgagcgc acggccaaga tgctcgccga ccacaaggtc gaggtcgacg
     2401 tcgacccgga cctgccgatg ctgcgcctcg actatctcct cttcgagcaa gtgctgttca
     2461 accttctcga caacgccgcg aaatacgcgc cgcccggctc gctcgtgcgc ctcagcgcgc
     2521 accgcgaggg cgtcgccgtc gtcgtcgaag tcatcgatga agggccgggc attccgccgg
     2581 ccgatctcga gcgcatcttc gacaagttct atcgcgtcta tgcccaggac cggcagcgcg
     2641 ccggcacggg cctcgggctt gcgatcggcc gcggcttcat cgaggcgctg ggcggcacga
     2701 tcattgccgg caaccgcgcc gaccgctcgg gcgcgatctt caccatccgt ctgccgatcc
     2761 gcggcgccga gcgcgtcgag cagcgacaag agggagcatg gctcgacgca ccaccgtcct
     2821 gatcgttgac gacgagccgc cgatccgccg gttcttgcgc accagcctcg ccgctcagga
     2881 ctacgaggtg atcgaggcgg ggaacggcgc cgaggcgatc cgcgcgatgg cgagcgcagc
     2941 gcccgatctg gtgattctcg atctgggact tcccgacatg aacggcaccg aggtgatccg
     3001 ctccatccgc ggctcctcgc ccgtgcccat catcgtgctc tcggtgcgca acgacgagcg
     3061 cggcaaggtc gaggccctgg atctcggcgc cgacgactat atcaccaagc ccttcggcat
     3121 ggaggagctg gtggcgcgca tccgcacggc gttgcgccat cgcttccagg agaagggcga
     3181 gactcccgtc ttcgtcgccg gggacctctc cgtcgatctc gtgcgccgca tcgtcaagcg
     3241 ctccggcgcc gaagtgaagc tctcgcccaa ggaatacgat ctgctgcggc tgctggtgga
     3301 gaatgccggc aaggtcctga cccatcgcca cctcctcgcc aaggtctggg gccctgccca
     3361 caccgaggac gcgcaatact tgcgcgtgtt catccgcagc ctgcgccaca agctcgaagc
     3421 cgatcccgcg cgccccgccc tcatcctcac cgagcccggc gtcggctacc gcctggcgag
     3481 cgcgctttag cgcgctcgcc aggcggcgtc agcggttcgc gcagcgaacc gcgggagccg
     3541 caccgatcgg attagctacg acgccgacga cgtttctcca aacctcaccc cagccggcgc
     3601 ttcacctcgt ccgggatcgg cgccgctttc atgcggccgg ggtcgtccgg atgcgggatg
     3661 gcgcagattc tgagctccca tccttccagg atgagcgtgg cgccgcgcaa gaagcggtgc
     3721 tgcaccttga aggtcttggc gctccactcg ctgacgcccg cttcgctgtc gagatcgtcg
     3781 ccgaaggagg ccgccgccgc gaacttcgct cccgcctcga ggagcgggaa acccttgagc
     3841 ccgtgcgtgc ggtaaagctg cggcaaaggg agtcccgcgc tttcgaacaa ggtcgaggtg
     3901 cactcgtcga accagcggaa gtagttcgga tagaaaacga tacccgcggg atcgcaatgc
     3961 ccccaatcca cgcggagtcg gcgtcggctg atgaacatgg tgctcgcccg gctgtccgcg
     4021 gcggattatg gccggaaccg tgaccgcggc gaaagagcgg tcgtgccgca gcgccaggat
     4081 ccgcagttgg tcgccattgg tcggatcgcc cgcaccggtg tctgcgacca gtcatttcgc
     4141 gcgaatgagc ttacccatct cccccgacgc cacctatgac ggtatgcgct ttcaagtggt
     4201 gtccttcatg gtttataatc catgatgacc taatctcgat gatgttgtgt aattgtctcc
     4261 taagtgcagt gatcgaagcg ctccgcgatg ggcccccctc atcgcggagc ttctcttttg
     4321 aggtccgcgc gcctatgcgc tcgcgttggg cgttcaagcg acgtcgcgac gt
//
LOCUS       DASYNS010000014        13008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_159455, whole genome shotgun sequence.
ACCESSION   DASYNS010000014 DASYNS010000000
VERSION     DASYNS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 13008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13008
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159455"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..343)
                     /locus_tag="VGP50_00475"
     CDS             complement(14..343)
                     /locus_tag="VGP50_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV8025866.1"
                     /translation="MAGTITVFGGTGFIGRHLVALLLRTGKTVCMAVHHPGRVKTPAE
                     ATNSPEIVQADMLDDTSVGSAIMGADAVFNLVGILTETSTQTYRAIHVKALAELLWQH
                     KIMGSCA"
     gene            complement(911..2878)
                     /locus_tag="VGP50_00480"
     CDS             complement(911..2878)
                     /locus_tag="VGP50_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV8025867.1"
                     /translation="MRRLINRLKIQHKLALLCSAFLLPIGFLIFLLVAHTEKDVSFAA
                     RELQGIAYIAALKTELEHVVALAYGSGSSADLASAQAGVLRFDAEGVDSAAAAAKAAE
                     AVRDAQRLPKGSTIMAYEPAFSAVLDHISRVADGSNLTLDPVLDSFYVQDLVTEKIPA
                     LVVAATRALSAALAIAASDHPSPEVTVQFLTRKGELAAVLNAVDADLTAGQRGNRDGT
                     MKPALDTPYAEVANRGAAYMQLLDGLASPGAPRPTTEALRNAAQALQQSASVFWEAAL
                     KELDHLVRARIASLNNSMAWSLALVCVVFLGSLALAWAIAGSISRPLYRLHQTMHALA
                     LGDLGVAVPDIDRRDEVGVMAKDVEVFKQDAIELARLADQVIASTRQIALATSQASSA
                     VGQVSEGANDQLEALRQVAVAVKQSTAAITDVSGSTQRAHELSASIAQQMNEGRERMR
                     ATADVVKGSAENAKQVEKMAQSISRIADQTQILALNAAIEAARAGEHSRGFAVVAEEV
                     RKLADHSGELAQEIGQVVRSATEESQNAVAMVEEVSSKMDSISESVRRSDTLTAAVAA
                     AMEEQQATVAQTNQTVDGLTRIAQSNATAAEEITSTIIDLSRLAEQSRGQVERSARAK
                     ENAPADVIWPQQAATSAAPGEGFRIPARRSY"
     gene            3112..4164
                     /locus_tag="VGP50_00485"
     CDS             3112..4164
                     /locus_tag="VGP50_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEV8025868.1"
                     /translation="MSGSARSPILAISDTEATFRGSRVRMDKRVRAQQAAAVEGEASS
                     TQAAARPPLADPRRGLLQRLSLDVERIAGIEITGPIQSKLARVLGSIDLAELEAWVAR
                     LHLLPAHDPEWLSLIESLTVHETYFYRDRAQLDFLGSEILPEIIVAAAASGRCRLRLW
                     SAGCATGEEAYTLAVLGLLALCDAGYAEERADRGIECRPPWTLEVLGTEISRLVLVQA
                     RAAVYPMTGVGAFRDLPRRLERFFPPLPRAEEKPDIELRGVHPAVRRHISFEQFNLMS
                     RLPPATGFDVVLCRNVLIYLSAAARRHVHGVLKQALRPGGFLLLGPTDVPAEPALFTT
                     CWGNGAAAYVLKLAHA"
     gene            4157..5488
                     /locus_tag="VGP50_00490"
     CDS             4157..5488
                     /locus_tag="VGP50_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEV8025869.1"
                     /translation="MPEPSVVFTLAGERLRLPVKKVELIAVPPAVTPVPHAPATLLGA
                     GQVGGQIVPIVDPAPLLHSLQARRRYDGSGQIVRVRSDGGRFGVWVDKVEAVTGGAQT
                     PGGPDADAVDLAADAPLLDIGGLAATGLTPPALGDAAGKAEAESAIPAPPAPPAAAVG
                     SYFVFEVAGRRFELAAEHVQQLVETVAWTRFPRAPRGIRGVAVLRGAALPLVSLATLL
                     GLPEAGEGGRFVVVAIDGHPLLLAVDRVLGLRVRQRGNGDGSSEDRRSAGEGELLDLA
                     EAMPDEIRRIVGGRAASVAATDEPTQQNGAQYLAISLAGEQFAVPITAVERVVGPRPI
                     FVLPQPPGAAIDGAPPIAGAIEFFGRIVPVLTLESRLGLRSGGAAPGAFMVVRGTCAI
                     AVDRIVRLVSLRPEEITPQPDENSILAGVAAPGGGPDLLPILAPGRLWADR"
     gene            5485..6603
                     /gene="cheB"
                     /locus_tag="VGP50_00495"
     CDS             5485..6603
                     /gene="cheB"
                     /locus_tag="VGP50_00495"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEV8025870.1"
                     /translation="MTKERIERSGRPLRVLVVDDLAFMRMALRRIIESDGDMRVVGEA
                     RNGEEAVAVACKLRPDVVTMDVEMPVMDGIVATRRILGEVEPQPVVIMVSSYTQAGAA
                     ATMKALHVGAADFVSKESALTKTDLGYIDAELRTKIRLWAGRRRLRPAAPAPRAETSP
                     DARADRSLARVPSGPVDLVAIAVSTGGPVTLARFLQALGPIAPPIVVAQHIPQFFTAG
                     LAHSLSEDTGLVVREGAERGPIVAGEVTILPGGRDALVALRRGGGFELRLTRSDAAAH
                     PSADLLFESAAMAARQPVAVVLTGMGNDGSRGAAAFARRNLPVLVQRPDTCIVGGMPG
                     AAIEAGVASESLTLEEIARRLRQWTPRSPPNREMANPR"
     gene            6600..7022
                     /locus_tag="VGP50_00500"
     CDS             6600..7022
                     /locus_tag="VGP50_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8025871.1"
                     /translation="MSTKKILVVDDSLIMRTIIKDIVASDPELEVVGFAENGKVGLQR
                     VRELKPDAILLDLEMPEMSGLEMLKRLMLLSRAKVIVVSSVGQTGSPQAMEARRLGAV
                     DVIAKPSGAMSLDLQDKKGHEIVQALRRALALAPGPGA"
     gene            7030..9909
                     /locus_tag="VGP50_00505"
     CDS             7030..9909
                     /locus_tag="VGP50_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEV8025872.1"
                     /translation="MDTLAETLWQEFAVEAEEHLQAIEPVLARADPEHIGEAEIAQLF
                     RSFHSVKGLARVLGLVGMEEVAHRAENLLGLVREGRAALTAERADLLLQSVDALKRMR
                     DRAIAERRDEPGDNALLARLAAAFAHAGGSEEEQQPPAGVAEARLELHDDPEMLAIFV
                     EMVKSRGSELCGGLSAEAGEREVAADAAATLGHAATVMGFDALAESFGGFERLLPPSD
                     TEPDERVREELLARLGDIRLQVELLGEMTGQDAGIGEFSAALTERIGADRRRLAAALT
                     DANRQLRAALATGDGPAAAEAAATVARLARALRAGVAAQSADHATEVLLLVEDLYSRV
                     AHGAPASTALADAADAVFAQIAEEGLDEAAVELTRLLRASFAGAGGRAAGGEGGARLV
                     AGLNLPDELLAILSDDNLAEFERGVLAVGLQPYAILAHLEADSDIAGHFIDWLSGEAR
                     AITNRTVITDGESSFEFLVLSPLMPNALAELLRALDPEHRCIRRVRRLTASAEGEPLL
                     DRAASERADAAARPSGFANLLRVRGELVDAFLDEIGELRISLSGLTEGARDADLRKEL
                     ARCRRFAERLPDEQRRELLPLLLRFEERDRWLRDTEERLSAGLSRLHQSALELRVVPV
                     EVVFNRLPRLVRDLAQRQGKSIELVVEGQEVRVDRSMVEALADPLIHLVRNAVDHGIE
                     PPEERRAAGKPERARLRLRAAKRGAEIDIEIADDGRGLDAEAIRAKAVARGLVTAARA
                     AELADEEIFQFIFAPGLSTAATVTETSGRGVGMDVVLNTVRRCNGNITLRSVRGRGTA
                     FVLTLPVSAALQSTLIVRINGQSLAIPEQHVIAVAEIEREAIRQVGAQRATRYRDSTL
                     PLYSLAGLLGITGEAPSAASSAQPIVVAGNGRQTIGLEVDEIEQRRELFLKPLHPLLA
                     RFPTIGGASVVGDGRVVLVLDGEQLLDLAARGIERAAPVEPLAAS"
     gene            9906..10301
                     /locus_tag="VGP50_00510"
     CDS             9906..10301
                     /locus_tag="VGP50_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEV8025873.1"
                     /translation="MSLYLKVAVGAGLYLLDAGQVIEIRAEGEKGRTWREQAVPILDC
                     RDLLGAAAGISRRAVLCFTGSGDSVELVVDRVEGLIELADRELHPLPQIGPLGELIDA
                     VVAIPGDDRLFLRLRAERMTTIAAGDGSP"
     gene            10427..12646
                     /locus_tag="VGP50_00515"
     CDS             10427..12646
                     /locus_tag="VGP50_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV8025874.1"
                     /translation="MAELTAVFQRNAAVFAASRAIENRAPLSEILSALTAALTEALRA
                     RLCRLVVADGAESIALRGDGCVGEEPRLPAVAGISRLVLDDGETRTTAPADDPACDPA
                     SDLPAGEKVRELMCVALPRSIGERIGVMQLFDKAEGGFTEADRRLAELAAPYLAEMLT
                     RAGLLRSTGSEIVAGRLVERVGPVEIVAGADLMLSKILAVALDVLNADRGSIFLHDKA
                     SDELVARHAEGLGGKEFRIDSWIGIVGAAFRTGLIVNVADAYQDPRFHPGLDWLTGYR
                     TRSVLCSPIVGNDGRPVGAVQLINKRGDSFTAADERRLRGLASQMGIAIDYAALFEQV
                     LAMKSYNESMLNSLTNGVVTLDLAGVVTYLNPAAARILRLGAARVVGRELAEAIGEFN
                     AWVGEAIGQDGGEASEKLLPNSEFYIESEDSWISVNLAIVPLRDTRQTPLGTMLVIED
                     VQRENELRRTMSRYLSNKVIDRLMVDTGAGLEGSSHEVTILFSDIRGYTPLTERLGAG
                     ETVRMLNEYFSFMEDVVTNRNGIIDKYIGDAIMAVFGSPFQSDDDAANAVRAATDMLR
                     VLDMLNARRRVDGKADIHIGVGIGTGICVTGNIGSPKRMDFTVIGDPVNLASRIETAT
                     KTYGAEIIVCGTTWSRLAERPRARRLDVVRLRGQTRPTELWEILEHRADIPDSAIEAY
                     DRGLASYFEGVWDRALPEFENALATRTGDKAARLMIERCRRSMETQPVDWQGVVELD"
BASE COUNT         1985 a         4416 c         4450 g         2157 t
ORIGIN      
        1 gccgacatgc gaatcacgcg catgacccca tgattttgtg ctgccaaagc aactcggcga
       61 gcgccttgac gtggatggcg cgataggtct gtgtggacgt ctcggtcaga atgccgacga
      121 ggttgaagac ggcatccgcc cccatgatcg cactgccgac gcttgtgtca tcaagcatat
      181 ccgcttggac aatctccggt gaattggttg cttcagccgg cgtcttaaca cggccggggt
      241 ggtgtacggc catgcacacc gtcttgccag ttcggagaag cagcgccacg agatggcggc
      301 cgatgaaacc ggttccgccg aacaccgtga tagtgccggc catggctcat ctccttcgtc
      361 gcggcgttgc tggcgtagcc atcaatcaat tccagccacc acacgctgac agaacctgta
      421 acaatagctc aagccggctg aactcctgct tgctcatcga caccaccgcc accgcacacc
      481 tcccaagacc ccgcgctcag ggggagtggc ggccgcatga tcatcatcga gagcttcggg
      541 cgtggcggcg caccgcgcgc cccgcccttg cccaaagccg ggctcggcac cgcgatgcca
      601 tgacgctcgt caccacttca tcgtactgca cacctgccgg ggaacctcgc ttccggcctt
      661 gcaccattgt cgccccgccg cctcccaagc caccccttac gccctcaatc aggccaagat
      721 cgccctctcg accgccgccg cggcgacagt cccgtcatcg caatcgtcga tccgacggca
      781 actacgccgg ccagcgcatc ccctactcaa gccgtcgccc ggccaaattc ccttagaggg
      841 cgaccgccta cacggcggcc cgcgggttcc ttcctgggag gctttcggac gtcggccctc
      901 agagcgcaca ctaatagcta cgacgggccg gtatccgaaa cccttcacca ggagcagccg
      961 atgttgcggc ttgttgcggc cagatcacgt cggcgggtgc gttctctttg gcgcgcgcgg
     1021 accgctcgac ctggccgcgc gattgctcgg ccaggcgcga caaatcgatt atcgtcgagg
     1081 tgatctcctc ggcggccgtc gcgttcgact gggcgatgcg ggtcaagccg tcgacggtct
     1141 gattggtctg tgcgacggtc gcctgctgct cctccatagc ggcggcgacg gcggccgtca
     1201 gcgtatcgct gcgccgcacg ctctcggaga tcgagtccat cttgctgctg acttcctcga
     1261 ccatcgcaac ggcgttctgc gattcctccg tcgcagaccg cacgacctgt ccgatttcct
     1321 gcgccagctc gccggaatga tcggccagct tgcggacctc ctcggccacg acggcaaacc
     1381 cgcggctgtg ctcgccggcc cgcgccgcct cgattgcggc attgagcgcc agaatctgcg
     1441 tctgatcggc gatccgcgag atggactggg ccattttctc gacctgcttc gcgttctcgg
     1501 cggagccctt gacgacgtcc gccgtcgcgc gcatgcgctc gcgtccttcg ttcatctgct
     1561 gggctatgct cgctgacagc tcgtgcgcgc gctgcgtgct gccgctcacg tcagtgatcg
     1621 ccgccgtact ctgtttgacc gccacggcga cctggcgcag cgcctcgagc tggtcgttgg
     1681 cgccttccga gacttgcccg accgcgctgc tcgcctggct cgtcgcaagc gcgatctggc
     1741 gcgtcgaggc gatgacctgg tcggcgagcc gcgccagctc aatcgcgtcc tgcttgaaga
     1801 cttcgacgtc cttcgccatt acgccgacct cgtccctgcg gtcgatgtcc ggcacggcga
     1861 cgccgaggtc gccgagggcg agcgcgtgca tcgtctggtg gagccggtag agcggccggc
     1921 tgatcgagcc ggcgatcgcc caggcgagcg cgagggaccc gaggaacacg acgcacacga
     1981 gcgcgaggct ccacgccatg ctgttgttga ggctcgcgat gcgggccctc acgagatggt
     2041 cgagctcttt aagcgcggct tcccagaaga cgctcgccga ttgctgtagc gcttgcgcgg
     2101 cgtttctcag cgcttcggtt gtgggccgcg gcgcgcccgg cgacgcgagc ccgtcgagca
     2161 gctgcatata ggccgccccg cggttcgcca cctcggcata gggggtgtcg agcgccggct
     2221 tcatcgtccc gtcgcggttg ccgcgctggc ccgcagtaag atcggcgtcg accgcgttga
     2281 gcaccgccgc gagctctcct tttcgggtga ggaactggac ggtcacttcc gggctcggat
     2341 gatcgctcgc ggcgatcgcc agggcagcgc tgagcgcgcg cgtcgcggcc acgacgagcg
     2401 ccggtatctt ctccgttacg agatcctgga cgtagaagct atcgaggacc ggatcgaggg
     2461 tcagattgga gccgtccgcc accctcgaaa tgtggtcgag gacggcgctg aacgcgggct
     2521 cgtaggccat gatcgtgctg cctttcggca atcgctgcgc gtcgcgcacg gcttcggcag
     2581 ccttggctgc ggcggccgcc gaatcgaccc cttcggcgtc gaacctcaga acaccggcct
     2641 gcgcgctggc caagtcagcg gacgagcccg aaccgtaggc gagtgcgacg acgtgctcga
     2701 gctcggtctt gagcgcggcg atataagcga tgccttgcag ctcccttgct gcaaagctga
     2761 cgtctttttc ggtatgggcg accaaaagga agatcagaaa accgatcggc agtaggaacg
     2821 cggagcagag cagcgcgagc ttgtgctgaa ttttcagtct gttgatcagg cgcctcatgg
     2881 gccgattcct caacattctt ccaagaccgg atagcggtgc agtgtggccg tccggcatgc
     2941 cgccggtctc cctccgcgtg acggtgtcct tcccccggcc ggtcaacaca actcgcagcg
     3001 cagggtaatc gctacacggc tacgccgccg ccggcaaagg cgccgggcgc cgagcccgca
     3061 agtcgaagcc gatccggagc ggagaatcat tcggctgccg attcgccgct gatgtcggga
     3121 agcgcccgtt cgcctatatt agcgatttcg gacactgaag cgacatttcg cgggagccgt
     3181 gttcgcatgg acaagcgggt gcgcgcacag caggcggcgg ccgtcgaggg cgaggcttcg
     3241 agcacgcagg cggcggcgag gccgcccctc gctgacccgc ggcgagggct gctgcagcgg
     3301 ctgagcctcg atgtcgagcg catcgccggg atcgaaatca ccggccccat tcagagcaag
     3361 ctcgcgcgag tgctcggctc gatcgatctc gccgagctcg aggcctgggt cgctcggctg
     3421 cacctgctgc cagcgcacga tccggaatgg ctcagcctta tcgaaagcct gacggtccac
     3481 gagacctatt tttatcgcga ccgcgcgcag ctcgatttcc tggggagcga gatcctgccc
     3541 gagatcatcg ttgcggcggc ggcgagcggg cgctgccggc tgcgcctgtg gtcagccggc
     3601 tgtgccaccg gcgaggaggc gtacacgctc gcggtgctgg ggctgctggc gctgtgcgat
     3661 gccggctatg ccgaggagcg ggcagatcgc gggatcgaat gccggccgcc ctggacgctg
     3721 gaggtgctcg gcaccgagat ctcccggctc gttcttgtgc aggctcgggc cgccgtttac
     3781 cctatgactg gcgtcggtgc ctttcgcgat ctgccgcgcc ggctggaacg cttctttccg
     3841 ccgctgccga gggctgagga gaagcccgat atcgagctgc gcggcgtcca cccagccgtg
     3901 cgccgccata tctccttcga gcagttcaac ctgatgtcgc gcctgccgcc agccaccggc
     3961 ttcgatgtcg tgctctgccg caatgtatta atctatttga gcgcagcggc gcggcgccac
     4021 gtgcatggcg tgctgaagca ggcgttgcgc ccgggcggct ttctgctgct cggcccgacc
     4081 gatgttccgg ccgagccggc cctgttcacg acctgctggg gcaacggggc ggcggcctat
     4141 gtgctgaagt tggcacatgc ctgagccctc ggtcgtcttc accctcgccg gcgagcggct
     4201 gcgattgccg gtcaagaagg tcgagctgat cgccgtgccg ccggccgtga cccccgttcc
     4261 gcacgcgcct gcgaccctcc tcggcgcggg ccaggtcggg gggcagatcg tgccgattgt
     4321 cgacccggcg ccgctccttc attccctgca ggcgcggcgg cgctacgacg gaagcggcca
     4381 gatcgtgcgg gtgcgcagcg acggcgggcg gttcggcgta tgggtcgaca aggtggaagc
     4441 cgtgaccggc ggcgcgcaga cgcccggcgg gccggacgcc gatgccgtcg atctcgctgc
     4501 ggacgcccct ctcctcgata ttggcgggct cgccgccaca ggcctgacgc cgccggcgct
     4561 cggtgatgcc gctggcaagg cggaagccga aagcgcgatt cccgcgcccc cggccccgcc
     4621 cgcagccgcg gtcgggtcct acttcgtctt cgaagtggcc ggcaggcgtt tcgagctggc
     4681 ggccgagcac gtgcagcaac tcgtcgaaac cgtagcgtgg acccgatttc cgcgcgcgcc
     4741 gcgcggcatc aggggcgtgg cagtgctgcg cggcgctgct ctgccgctcg tctctctcgc
     4801 gacgctgctc ggcctgccgg aagcgggaga gggcggccgc ttcgtggtag tggcgatcga
     4861 cggccaccct ttgctcctcg ccgtcgatcg agtcctcggg ctgcgcgtac gccagcgtgg
     4921 caacggcgac gggtcgagcg aagaccgccg atccgccggc gagggcgagc tgctcgatct
     4981 tgccgaggcg atgcctgacg agatccgccg cattgtcggc ggtcgcgcag cgagcgttgc
     5041 cgcgaccgac gagccgactc agcaaaacgg ggcgcaatac ctcgcgatct cgctcgccgg
     5101 cgagcaattc gcggtgccga tcacggcggt cgaacgcgtc gtcgggccgc ggcccatatt
     5161 cgtcctgccg cagccgccgg gcgccgctat cgacggcgct ccgccaatcg ccggcgcgat
     5221 cgagttcttc ggacggatcg tgccggtgct gacgctcgaa agccgcctcg ggcttcgcag
     5281 cggcggcgcg gcgcccggcg ccttcatggt cgtgcgcggg acttgcgcga ttgccgtcga
     5341 ccgcatcgtc cgcctagtgt cgcttcggcc ggaagagatc acgccgcagc ccgacgagaa
     5401 cagcattctc gccggcgtgg cggcgccggg cggcggcccc gatctgctgc cgatcctggc
     5461 gccaggacgg ttatgggccg atcgatgacg aaggaacgca tcgagcggtc ggggcgcccg
     5521 ttgcgcgtcc tcgtcgtcga cgatctcgcg ttcatgcgca tggcgttacg ccggatcatc
     5581 gagagcgacg gcgacatgcg cgtcgtcggc gaagcgcgca acggcgagga ggcggtcgct
     5641 gtcgcttgca agctgaggcc ggacgtcgtg acgatggatg tcgagatgcc ggtcatggac
     5701 ggcatcgtgg cgacgcgccg gatcctcggg gaggtcgagc cgcagccggt cgtcatcatg
     5761 gtcagcagct acacgcaggc tggcgcagcc gcgacgatga aggcactgca tgtcggcgcc
     5821 gccgatttcg tctcaaagga gtcggccctg acgaagaccg atctcggtta tatcgacgcc
     5881 gaactgcgca cgaagatccg gctctgggcc gggcgccgcc ggcttcggcc ggcagcgccc
     5941 gctccgcggg cagagacgtc ccccgatgca cgagccgacc gcagcctggc ccgcgtgccg
     6001 agtggaccgg tcgatcttgt cgcgatcgcc gtatcgaccg gcgggccagt gacgctcgcg
     6061 aggtttctgc aggcgctcgg ccccatcgcg ccgccgatcg tcgtcgccca gcacatcccg
     6121 caattcttta ctgccgggct cgcccactcg ctgagcgagg ataccgggct tgtcgtgcgc
     6181 gaaggcgcag aacgtggtcc gatcgttgcc ggcgaggtga ccatcctgcc cggcggccgg
     6241 gacgcgctcg tcgcgctgcg ccgcggcggc ggtttcgaat tgcggcttac gcggtccgac
     6301 gcggcggcgc atccctcggc cgacctgctg ttcgaatcgg cggcgatggc ggcgcgccag
     6361 cccgttgccg ttgtcctgac tggcatgggc aacgacggga gccgcggcgc ggcggccttc
     6421 gcgcgccgca accttccggt actggtgcag cgccccgata cctgtattgt cggcggtatg
     6481 ccgggggcgg ccatcgaggc cggcgtcgcc agcgagtcgc tgacgctgga agagatcgct
     6541 cgccgattgc gtcagtggac cccccgatcg cctcccaatc gcgaaatggc caatccgaga
     6601 tgagcacgaa aaagatcctt gtcgtcgacg attcgctgat catgcgcacg atcatcaagg
     6661 acatcgtcgc gagcgatccc gaactcgaag tcgtcggctt tgccgagaac ggcaaggtcg
     6721 ggctgcagcg ggtccgcgaa ctgaagcccg acgcgatcct gctggatctc gaaatgccgg
     6781 aaatgtcggg ccttgagatg ttgaagcggc tgatgctgct cagccgggcg aaggtgatcg
     6841 tcgtctcctc ggtcggacag actggatcgc cgcaggctat ggaagctcgc cgactgggcg
     6901 ccgtcgacgt gatcgccaag ccctccggcg cgatgagcct cgacttgcag gacaaaaagg
     6961 gacacgagat cgtgcaagcg ctacgccgcg cattggctct cgcccccggc cccggcgcct
     7021 gagctgccga tggatacgct ggccgagacg ctgtggcagg aattcgcggt cgaggccgag
     7081 gagcatctgc aagccattga accggtgctc gcgcgcgccg atccggagca catcggcgag
     7141 gccgaaatcg cccagttgtt tcgctccttc cattcggtca aagggctcgc gcgcgtcctc
     7201 gggctcgtcg gcatggagga ggttgcgcat cgcgccgaga acttgctcgg gctcgtacgc
     7261 gagggccgcg ccgctctgac ggccgaacga gccgatctgc tgttgcaatc ggtcgacgcc
     7321 ttgaagcgga tgcgcgaccg ggcgatcgcc gagcgccgcg acgagccggg cgacaacgct
     7381 ctgctggcgc ggctggccgc cgctttcgcg cacgccggcg ggagcgagga agagcagcag
     7441 ccgcccgccg gcgtggcaga agcgcgcctg gagctgcacg acgatcccga aatgcttgcg
     7501 atcttcgtcg agatggtgaa gtcgcgcgga tcggagctgt gcggcgggct gtcggcggag
     7561 gcgggcgagc gggaggtcgc ggccgacgcc gccgcgacgc tcggccacgc cgccacggtg
     7621 atggggttcg acgcgcttgc cgagagcttc ggcgggttcg agaggcttct gccgccgtcc
     7681 gacaccgagc ccgacgagcg ggtgcgcgaa gagctgctgg cgcggctagg cgacattcgt
     7741 ctgcaggtcg agctgctcgg cgagatgacc gggcaggatg ccggcatcgg cgaattttcc
     7801 gccgcgctca cggagcgcat cggcgcggat cggcgccggc ttgcggccgc gctgacggac
     7861 gccaaccggc agctgcgggc tgcccttgcc acgggggacg ggcccgccgc cgccgaggcg
     7921 gccgcgaccg tggcgcgact cgcgcgtgcg ctgcgcgccg gcgtcgcggc gcagtcggcc
     7981 gaccacgcca ccgaggtact tcttctcgtc gaagacctgt acagccgcgt ggcgcacggg
     8041 gcgccggcat caacggcgct cgccgatgcg gcggatgctg ttttcgcgca gatcgcggag
     8101 gaggggttgg acgaggcggc ggtggagctg acccgtctct tgcgcgcctc cttcgcgggc
     8161 gcgggcggtc gagcggcggg aggcgagggc ggggcccgcc tcgtcgccgg ccttaacctc
     8221 ccggacgagc tgctcgccat actgtcggac gacaacctcg ccgagttcga gcgcggcgtc
     8281 ctcgcggtcg ggctccagcc ctatgcgatc ctcgcgcatc tcgaggccga ttcggacatt
     8341 gccgggcact tcatcgactg gctctcgggc gaggcgcgcg cgatcacgaa ccgcaccgtc
     8401 atcaccgacg gtgagagctc gttcgagttc ctcgtcctct cgccactgat gccgaacgcg
     8461 ctcgccgagc tactgcgggc gctcgacccc gagcaccgtt gtatcagacg cgtgcggcgc
     8521 ctgacggcca gcgccgaggg tgagccattg ctcgatcgcg ccgcgtcgga gcgggcagat
     8581 gcggccgctc ggccgagcgg cttcgccaat ctgctccggg tgcgcggcga gctcgtcgac
     8641 gcctttctcg acgagatcgg cgagctgcgg atctcgctga gcggtctgac cgaaggcgct
     8701 cgcgatgccg acctgcgcaa ggaattggcg cgctgccgaa ggttcgccga gcgtctgccg
     8761 gacgagcagc ggcgcgagct gctgccgctc ttgctgcgct tcgaggagcg cgaccgctgg
     8821 ctccgtgata ccgaggagcg gttgtccgcg gggcttagcc gcctgcacca gagcgccctt
     8881 gagctgcggg tcgttccggt cgaggtcgtc ttcaaccggc tgccgcgcct cgtgcgggac
     8941 ctggcacagc ggcaggggaa atcgatcgag ctggtcgtcg agggccagga ggtgcgggtc
     9001 gacagaagca tggtcgaggc gcttgccgac ccgctcattc atctggtgcg taacgccgtc
     9061 gatcacggca tcgagccgcc ggaggagcgg cgcgccgccg gcaagccgga acgcgcgcgg
     9121 ctgcggctgc gcgcggcgaa gcgcggcgcc gagatcgaca tcgagatcgc cgatgacggg
     9181 cgcggcctcg atgccgaggc gatccgcgcc aaggcggtcg ctcgcggtct cgtcacggcg
     9241 gcccgggcag cggaactcgc ggacgaggag atctttcagt tcatcttcgc gcccggcctg
     9301 tcgaccgccg cgaccgtcac cgagacctcg gggcgcggcg tcggcatgga tgtcgtgctc
     9361 aacacggttc ggcgctgcaa tggcaacatc acgctgcggt ccgtgcgcgg ccgcggcacc
     9421 gcgttcgtgc tgacgctgcc cgtatcggcc gcattgcaga gcacgctgat cgtccgcatc
     9481 aacgggcaga gtctcgcgat cccggagcag cacgtgatcg ccgtcgcgga gatcgaacgc
     9541 gaggcgatcc ggcaggtcgg cgcgcagcgg gcgacccgct accgcgactc gaccctgccg
     9601 ctctacagcc tcgccgggct gctcggcatc accggcgagg cgccgtccgc cgcctcgtcg
     9661 gcgcagccga ttgtcgtcgc cggcaacggc cgccagacga tcggtctcga agtcgacgag
     9721 atcgagcagc ggcgcgagct gttcttgaag ccgctgcacc cgctgctcgc ccgcttcccg
     9781 acgatcggcg gcgcctcggt ggtcggcgac gggcgggtcg tgttggtcct cgacggcgag
     9841 caattgctcg acctcgccgc gcgcggcatc gagcgtgccg caccggtcga gcctctggcg
     9901 gcgtcgtgag cctctatttg aaagtggcgg tcggggcggg gctctacttg ctcgatgccg
     9961 gccaagtgat cgagatccgc gcggaggggg agaagggccg cacctggcgc gagcaggcgg
    10021 ttccgatcct tgactgtcgc gatctcctcg gagcagccgc cgggatctcc cggcgcgccg
    10081 tcctctgttt caccggctca ggggattcgg tcgagctcgt cgtggatcgg gtcgagggtc
    10141 tcatcgagct cgccgaccgc gagctccacc cgctgccgca gatcggcccg ctcggcgagc
    10201 tgatcgacgc cgtcgtcgca atccccggcg acgatcgcct cttcttgcgg ctgcgcgccg
    10261 aacgcatgac gacgatcgcc gccggggacg gttctccatg aacccggcgg acccgatcgc
    10321 cttcgactac gagcagctgg cggcacagct ggtcgcgcac cgcctatcgc agccggatgt
    10381 gacggcgctg ctggatccgg ggtcgcccga gcggcgccgt gccgcgatgg cggaattgac
    10441 cgccgtgttc cagcgcaatg ccgcggtctt cgcggcctct cgcgcgatcg agaaccgggc
    10501 gccgctcagc gagatcttgt cggcgctgac ggcggcgctg accgaagcgc tgcgcgcccg
    10561 gctctgccgt ctcgtcgtcg ccgacggcgc ggaatcgatc gcgttgcgcg gcgacggctg
    10621 cgtcggcgaa gaaccgcggc tgccggcggt cgccggcatc tcgcgcctgg tgctcgacga
    10681 cggcgaaacc cgcaccaccg ctccggcgga cgaccccgcc tgcgatcccg cgagcgacct
    10741 gccggccggg gagaaggtgc gcgaactgat gtgcgtggcg ctgccgcgct cgatcggcga
    10801 gcgcatcggc gtgatgcagc tgttcgacaa agcggagggg ggcttcaccg aggccgaccg
    10861 ccggctcgcc gaactcgccg cgccctatct cgccgagatg ttgacgcgcg ccggactgct
    10921 gcgctccacc ggcagcgaga tcgtcgccgg tcggctggtt gaacgggtcg gaccggtcga
    10981 gattgtcgcc ggcgccgatc tgatgctgtc gaaaatcctc gccgtcgcgc tcgacgtgct
    11041 caatgccgac cgaggctcga ttttcctcca cgacaaggca tccgacgagt tggtcgcgcg
    11101 ccacgccgag gggctgggcg gcaaggaatt tcgcatcgat tcctggatag gcatcgtcgg
    11161 cgccgccttc cgcacggggc tcatcgtcaa tgttgccgac gcctatcagg atccgcgctt
    11221 ccaccccggc cttgactggc tgaccgggta tcggacgcgc agcgttctct gctcgccgat
    11281 cgtcggcaat gacggacgtc ccgtcggcgc ggtgcagctg atcaacaagc gcggcgacag
    11341 tttcaccgcc gccgacgaac gccgcctgag aggcctcgcc tcgcagatgg gaatagcgat
    11401 cgactacgcc gcgctgttcg agcaggtcct cgcgatgaaa tcctacaacg agagcatgct
    11461 gaacagcctg acgaacgggg tcgtgacgct cgatctcgcc ggcgtcgtga cctacctcaa
    11521 tccggctgcg gcgcgcatcc tgcgcctcgg tgcagcgcgc gtggtcggcc gcgagttggc
    11581 ggaggctatc ggtgaattca acgcctgggt tggcgaagca atcggacagg acgggggcga
    11641 agcgagcgag aagttgctgc cgaacagcga attctacatc gagagcgagg acagctggat
    11701 ctccgtcaat ctggcgattg tgccactgcg cgatacgcgg cagaccccgc tcggcacgat
    11761 gctggtgatc gaagacgtgc agcgcgagaa cgagctgcgc cgcacgatgt cgcgctacct
    11821 ctcgaacaag gtgatcgacc ggctgatggt cgacaccggc gccggcctgg aaggcagctc
    11881 gcacgaggtg acgatcctgt tttccgacat caggggctac acgccgctca ccgaacgcct
    11941 cggcgccggg gaaacggtgc ggatgctgaa cgaatatttt tcattcatgg aagacgtcgt
    12001 gaccaatcgc aacggcatca tcgacaagta cattggcgat gcaatcatgg cggtattcgg
    12061 ctcgccgttc caaagcgacg atgacgcggc gaatgcagtg agggccgcca ccgacatgtt
    12121 gcgcgtactc gatatgctta acgcgcgccg ccgcgtcgac ggcaaagccg acatccatat
    12181 cggcgtgggg atcggcaccg gcatttgcgt caccggaaat attggctcgc ccaagcggat
    12241 ggatttcacc gtgatcggcg atccggtcaa tctcgcctcg cgcatcgaaa cagcgaccaa
    12301 gacatacggc gccgagatca tcgtatgcgg aaccacctgg tcccgcctcg ccgagcggcc
    12361 gcgcgcgcgc cggctcgacg tcgtccggct gcgcgggcag acccgcccca ccgagctttg
    12421 ggagattctc gagcaccgcg cggatatacc cgatagtgca atagaagcct atgatcgcgg
    12481 gctcgccagc tatttcgaag gtgtgtggga tcgggcattg ccagagttcg agaatgcgct
    12541 cgcgacccgg actggcgaca aggcagcacg gctgatgatt gagcgctgcc ggcgatctat
    12601 ggaaactcaa ccagtcgatt ggcagggagt ggtggagctg gattaacagc ttgggcgatc
    12661 tgccggtcaa ggcccgcaac tgatttggcg gctcgccggg tccttgctat ccgctagccc
    12721 cgtgctgtcc cgagcgccac ggtatgctga acggccaggc gtccgggcaa ttttggatga
    12781 tccgccgatc cgcatccgcc cgattttccc gcacagggcg ggtgcaaagg aaacgaaccc
    12841 tgtggagacc ctactgtggc cctcaggatt tcccgcccga tccccgacac cggtccgctc
    12901 gcaatcccgc cgaagtctta caccgccgcg gactcgctac tcctgaagga ctgcgccggc
    12961 gtcaagcccg gcacggcctt cactggccat tcttttgcca gcaccgtt
//
LOCUS       DASYNS010000015        15197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_627841, whole genome shotgun sequence.
ACCESSION   DASYNS010000015 DASYNS010000000
VERSION     DASYNS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 15197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15197
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627841"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..647
                     /locus_tag="VGP50_00520"
     CDS             321..647
                     /locus_tag="VGP50_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV8025875.1"
                     /translation="MYRLLLALTILTAIIAAPAMAEDSGVTITIKDHRFVPDMVEIPA
                     GTKVKIVVRNEDTTTSEFESVAFHREKVVQPGSEITLFVGPLDPGSYEFFDDFHPETR
                     GHLVAK"
     gene            660..1499
                     /locus_tag="VGP50_00525"
     CDS             660..1499
                     /locus_tag="VGP50_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEV8025876.1"
                     /translation="MLATLIIVFREVLEAALIVGIVLAASRGVPRRRLWISGGIAGGV
                     LGALIVASLAGEIASAVEGVGQELFNAGILFAAVGMLGWHNIWMQRHGRELAQSATEI
                     GRAVLAGTRPLYALAVVVGVAVLREGSEIVLFLYSIAVAGGSSATSMIAGGVLGLAVG
                     VGAGAGLYLGLLAIPLRHLFTVTSWLILFLAAGMASQGAAFLLQADLLPALGNNLWDT
                     SFLLSERSLPGQVVHVLLGYSAQPAGIQVVFYLATLVIIGSLMRLLGGMPAASPRRDL
                     ARS"
     gene            1546..2328
                     /locus_tag="VGP50_00530"
     CDS             1546..2328
                     /locus_tag="VGP50_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025877.1"
                     /translation="MSAKLSIGAAVSVWSLSLLYAGPAAADFKVITPDANSGELELET
                     VGDAGFDPNRNKSGEQSYTSELEYGVTSWWQTELEVEFNRAAGAGQDIFFNQVTSENV
                     FQFTERGEYWLDAGFFAEYGQSTLRNAPSGTTFGPILRKDFWGTSTTVDLFLEKDLGD
                     HAAGRPVFSFAAETRLDSLVMQFGRHVAVEPGFQIYGTPGAIGHFARWGQQDERAGPQ
                     LFGKIFNIGPGTLEWNGGVLFGLSPAVPAVTPRWQIEYEIHY"
     gene            complement(2354..2476)
                     /locus_tag="VGP50_00535"
     CDS             complement(2354..2476)
                     /locus_tag="VGP50_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025878.1"
                     /translation="MSKVKTAVVLVLMGIGIAGSLTGCFVEDGHGDGWHHHDWR"
     gene            complement(2590..3300)
                     /locus_tag="VGP50_00540"
     CDS             complement(2590..3300)
                     /locus_tag="VGP50_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025879.1"
                     /translation="MPTSSPVADYLDALSRALSFDAALARCVRCEVEDHLLEAAAAAA
                     DGREPSVEAQHRALARFGDASDIAQGYVAASLFRQTRRNGLVALLVVAGIFCVMKLRM
                     AYYGPAPLEFAEDVQRIRALALPISGYAFKLALVSAVLGAVYIACRRVPARFHRAYDR
                     ELRRYFGLCAVAVAAVITSVLSDAVLTATRLSAAAPPSALVPLLSMAAEIAFAGGLIL
                     HLRRTLRHMACAVTLSRI"
     gene            complement(3275..3628)
                     /locus_tag="VGP50_00545"
     CDS             complement(3275..3628)
                     /locus_tag="VGP50_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV8025880.1"
                     /translation="MEGGEILKGHMDMIVLAALQAGPAHGYAVIQEIHRRSGGAFDLP
                     EGTIYPVLHRLEQTGLLASRWITAESGRQRRVYALTPRGRRALADRRAVWRRFTEAIG
                     GLLGDLGHANIESGR"
     gene            complement(3848..4291)
                     /locus_tag="VGP50_00550"
     CDS             complement(3848..4291)
                     /locus_tag="VGP50_00550"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV8025881.1"
                     /translation="MYRILCFATALVLFGPAARADTAEQETNKKTVLEFYEAGINQKD
                     FAKAAQYFGPRYTQHNPNAADGPEGFKHFLDFLREKFPNSHSEIKQVFADGDYVILHV
                     HAMREPGSRGSAIVDIFKLEKGKIVEHWDVVQPIPEQAANSNGMF"
     gene            4470..7238
                     /locus_tag="VGP50_00555"
     CDS             4470..7238
                     /locus_tag="VGP50_00555"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEV8025882.1"
                     /translation="MSEIQNQRDIIDRRAVSAALDALADGGAEPPRTKVVEVLRGALQ
                     QGRAEVRRRFDERGKGGATVRELSFLMDQLVRALYDFVGAHVYPLANPTEGERLAVVA
                     VGGYGRGELAPYSDIDLLFLLPYKPTPHTEQVVEYMLYAMWDLGVKVGQATRSVEETL
                     RQSKTDLTIRTAVLEARYIWGDEKLFLELKRRFDAEIIKGTAAEFVQQKLAERDQRHA
                     RMGDSRYVVEPNVKEGKGGLRDLHTLFWIAKYIYRVDDVEKLVDLNVLSAKEAHRFMR
                     AQDFLWTVRCHLHFLTERAEDRLTFDRQTEVGRRMRYTDHAGTRGVERFMKHYFLIAK
                     DVGDLTRIFCAQLEAEQEYRPRFSWRPWSGRKRLIEGFAVDAGRLQVARESTFRDDPV
                     NLIRLFHVAQEHGLDIHPRALRLVTQSLKLIDADLRANPEANRLFMEILTSRNEPEIA
                     LRRMNEAGVFGRFVPDFGRVVAQMQYDMYHVYTVDEHSLFAIGILHKIEAGALKDELP
                     LASDIVRTIDSRRALYLAVLLHDIAKGRGGDHSELGAQVAERLGPRLGLAADETETVA
                     WLVRFHLLMSNTAFKRDIDDPQTIRDFVERVQSPERLKLLLILTAADIRAVGPRVWNG
                     WKATLLRELYHQAIEEMSGGLVADSRDARIAAAQNAARALLPDFTAEEMAAFIAKGYP
                     SYWLSFDSATHARHARLTREAERTNAPLTVDTRVDTRRAVTEVTLYTADHAGLFSRIA
                     GALALAGANIVDAKILTMSNGMALDTFWVQDQSGGAFDRGDKLARLAVMFENVLSGRT
                     KPHLELAKPDPIMSRTRVFTVAPRVLIDNGASLTHTVIEVNGRDRPGLLFEVTRALTA
                     LNLQISSAKISTYGEKVVDVFYVKDLFGHKVEHERRLKDIREHLVAVLAEPAAAAARA
                     RAKTIAAE"
     gene            7480..7869
                     /locus_tag="VGP50_00560"
     CDS             7480..7869
                     /locus_tag="VGP50_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025883.1"
                     /translation="MTSAAVVLLGLVVNAGHAAGPFDGTWQLDAPGAGGSSPEAGSKC
                     PAVRLRFEIKDNQVEGRFERSPDLRHVETGEGHEGTPVTGSVGPDGTLTTKWEGINGS
                     GKLAGNGGQLKWIGDCGPRVGTLARVS"
     gene            8075..8464
                     /locus_tag="VGP50_00565"
     CDS             8075..8464
                     /locus_tag="VGP50_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025884.1"
                     /translation="MRLAKWVFRVAGVYGIVMLAPAYFLETTFTDSLHPEFYYGFLGV
                     GLAFQALFLMLASDPSRYRPMMIPAILEKVSYGVATFWLFFADRADSSVLLGGCVDWL
                     FVGLFAWAYLATAPSQEVQRGLRVRAA"
     gene            complement(8599..9045)
                     /locus_tag="VGP50_00570"
     CDS             complement(8599..9045)
                     /locus_tag="VGP50_00570"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV8025885.1"
                     /translation="MARSYYSTVLDEPAGMVWSVIRDFGNYALWVDGVDDALIEDERP
                     GDAVGAVRSVRMGETRTRQKLLAHSDRERSYTYEFCEPCHFPVRDYVATIRVTPIVDG
                     NRAFVEWWATFDVAVGDGNHWTKFFARSFATWLGRLRRGLAGDSLR"
     gene            complement(9152..10864)
                     /locus_tag="VGP50_00575"
     CDS             complement(9152..10864)
                     /locus_tag="VGP50_00575"
                     /inference="COORDINATES: protein motif:HMM:NF019874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEV8025886.1"
                     /translation="MRTTDGKSRRRGLRDLPLSIKLQSLVVLTLAALAVMASVAVITQ
                     YDRMREDRITELHAAIDVARSFAESLDADVKAGKLTKPEAIELFRRQIDHAFYGHKDY
                     YFVYTMDGTIVARASDPENEGKNLIDFKDKSGRLIIRDQAELAQKGGGPYTVYWTRPG
                     ETEPVEKVNYVAEIPWDMWIGTGVFMDDLGASVRNTLMHFIAIAAAVGAVASLIVWLV
                     ARGIATPLRRLDRDMTMLASGNLSIEIADTGRRDEVGRMVRSVAVFKTNALEKQRLET
                     QRQNSEAAAAAERRRFLESIADQLHGKIGSLTESLSAASQRLKRTAETMSTAAAQSDR
                     QSLAISSAVGQAAVNVETVATSAAQLTSSIHEIGRQVEQSTAIAGRAVGDAKRTDAIV
                     HELSASAQKIGRVVELINEIAGQTSLLALNATIEAARAGDAGRGFAVVASEVKALANQ
                     TARAIDDIGGQITQIQTATTEAVGAIEAIAGTIGDISTTVSTISAAIEEQQAATREIS
                     RNAEEASRGTEAAGNNVTGIREAVMRTAGASGEVLSAAQELANQSEALSQELVRVIGQ
                     IRAA"
     gene            complement(11017..11568)
                     /locus_tag="VGP50_00580"
     CDS             complement(11017..11568)
                     /locus_tag="VGP50_00580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8025887.1"
                     /translation="MRKELAARLAVEADAPVLRAVMDAAIAENLKPFLTPELIAASHA
                     IMGLDTTLISDRTYWIVYGGAAVAGCGGWSRRATIYGGDHSAGRNAALLDPAVDAARV
                     RAMYTHPSWVRRGVGRLILRLSEEAAAREGFSRLELVATLSGEPLYRAYGFTVLERLE
                     DGGVPLVRMAKTIAAEFLADLPE"
     gene            11957..12691
                     /locus_tag="VGP50_00585"
     CDS             11957..12691
                     /locus_tag="VGP50_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV8025888.1"
                     /translation="MDVQFLGSGDAFGSGGRFNTCIHVATAGGAFLIDCGASSLVALR
                     KFGVDPNGIHTVFVSHLHGDHFGGLPFLLLDAQFYSRRNAPLTLVGPPGFRERLTQAM
                     EVFFPGSSRVQRKFATEIVEIEAGTTSAINGVDVTAHLVAHACGAPPLALRLSCERKV
                     IAYTGDTEWTETLLELGRDADLLIAEALFFARKIKHHLDYATLKANLVRIGAKRVVLT
                     HMGPEMLEHRADVPEDVAEDGKIIRL"
     gene            complement(12731..13726)
                     /locus_tag="VGP50_00590"
     CDS             complement(12731..13726)
                     /locus_tag="VGP50_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025889.1"
                     /translation="MPLGRTLAGAYGFVFNNVVGFLVLAGVPWLGISIAGLFTASAKP
                     DAYVLMVVAFFPWLARRLASMPAPAVLLHTPDVVSFLLHIFFVSAFALAWHRYVLTGS
                     VTSGVGALRLLFRDLRFFAYCLFVLLAIPLLVSGGLGLFVLSYGIAAGVGFGFALLSL
                     LLWKVARRPVGAFLALFLIAAGAVIFHEGMPESLRSFFLVAVIFVAATPLARLLLAFP
                     AIAMGEPGALPLTAWHRSRGHDAKLCLGLIGCAAPFEAARLGALFLLTAYAGHAGRAA
                     AHSAAIALMPLSNVWFLLEIAVASSFLCIAYRQLGGSETPASPDEVLAAEGLPSA"
     gene            13946..15007
                     /locus_tag="VGP50_00595"
     CDS             13946..15007
                     /locus_tag="VGP50_00595"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEV8025890.1"
                     /translation="MTAAGPTLINTDFRKAIERNKDNTLLLIEVLAVSAAVVGYVFGW
                     GLAVIRDIWAMPPAALSRLDVAWILRDLFTVPPRSEALIGAGTMLAASIVWGVVMLYA
                     GAHILSSFARTRAANPEKASEQLFIDVVKEMALAAGLPAPHALVIDTLALNAFTVGSS
                     PEHAVIAVTSGLLQACSREELQGVVAHEMGHIADYDMRYATLVAAAASTVVLVCHLLW
                     GARGTSMWRGGGTAATRAGLTLAVLIVMLAVSIVAPLAARLVQFAISRQREYLADATS
                     VKLTRDPAGLIHALQRLEQGNTDLAHAGSPLLALCIAAPKSEVPGDLLASHPPIADRI
                     ARLRNLGEIAAPAIAASSG"
BASE COUNT         2579 a         5030 c         4971 g         2617 t
ORIGIN      
        1 agacgaggac gcggtcggct cggcggattt tcgccgcgga ttttcgcaac ctgtcgctgc
       61 tcggcgcccg gggttccgcg ctccgcagac ggtgccgcgg cgctgccgtc ctgcgcggaa
      121 cgtcatcgga cgtcgaggtc gagggagccg tcgggagatc ggtggcggag ggaggatttg
      181 cacctctgcc cgctcttcgc ccgcaagaca ggggaatacc tggggcgtcg tcggtatcag
      241 ggacttgacg gcgaagaaag ccaaccggta ggattgagaa taagtcgcag ttgcgcgctt
      301 ttagggatgt ggaacagtcc gtgtatcgcc tcttgctcgc cctgacgatt ctgaccgcga
      361 tcattgcggc accggccatg gccgaggata gcggcgtcac cattacgatc aaggaccatc
      421 gcttcgttcc cgacatggtc gagattccag cgggaacaaa ggtcaagatc gtcgtccgca
      481 acgaggacac gacgacctcg gaattcgaaa gcgtcgcgtt ccatcgcgag aaggtggttc
      541 aaccgggcag cgagatcacg ctgttcgtcg gccccctcga ccccgggagc tacgagttct
      601 tcgacgattt ccatcccgaa acgcgcggcc atctggtcgc gaaataaaga gggtccggca
      661 tgctcgcgac gctgatcatc gtcttccgcg aggttctgga agctgccctc atcgtcggca
      721 tcgtgctggc cgcgagccgc ggcgtgccgc gccgccggct gtggatctcg ggcggcatcg
      781 ccggcggggt cttgggcgct ctcatcgtcg cgtcgctcgc cggcgagatc gcgtccgcgg
      841 tcgaaggcgt cggccaggag ctgttcaacg ccgggatcct gtttgccgcc gtcggcatgc
      901 tgggatggca caacatctgg atgcagcgcc acgggcgcga gctggcgcag agcgcgacgg
      961 agatcggccg cgcggtgctc gccgggacgc gtccgctcta tgcgttggca gtggtggtcg
     1021 gcgtcgccgt gctgcgggaa gggtcggaga tcgtgctgtt cctctacagc atcgccgttg
     1081 ccggcggcag cagcgcgacc agcatgattg ccggtggcgt gctggggctc gccgtgggcg
     1141 tcggcgccgg ggccggactt tatctcggcc tgctcgccat tccgcttcgc catctgttca
     1201 cggtgacgag ctggctcatt ctgtttctcg cggccggaat ggcctcgcaa ggcgccgctt
     1261 ttctgcttca ggctgacctc ttgccggcgc tcggcaacaa tctctgggac acgtcatttc
     1321 tcttgagcga gcgcagcctg cccggtcagg tcgtgcatgt cctgctgggc tacagcgccc
     1381 agccggcggg catccaggtc gtgttctacc tggcgaccct cgtcatcatc ggctcgctga
     1441 tgcggctcct cggcggcatg cccgcggcgt cgccgagacg agacctggcc aggtcataat
     1501 cggtgggcgc ctcgggcgcc gcgtcgcgct gagctggggg aacatttgag cgcgaaactc
     1561 tcgatcggcg ctgcggtttc ggtctggtcg ttgtcgcttc tctatgccgg cccggcggcg
     1621 gccgacttca aggtcatcac gccggatgcc aatagcggcg aattggagct cgagaccgtc
     1681 ggcgatgccg gcttcgatcc caaccgcaac aagagcggcg agcagagcta tacctcggag
     1741 ctcgaatacg gcgtgacgag ctggtggcag actgagctcg aggtggagtt caaccgcgcc
     1801 gcaggcgccg gccaggacat cttcttcaac caagtcacct cggagaacgt gttccagttc
     1861 accgaacgcg gcgagtactg gctcgatgcc ggattcttcg ccgagtacgg ccaatcgacg
     1921 ctgcggaacg cgccaagcgg gacgaccttc ggcccgatcc tccgcaagga tttctggggc
     1981 accagcacga cggtcgactt gttcctcgaa aaggatctgg gcgaccatgc cgcgggacgg
     2041 ccggtcttca gctttgccgc ggagacccgc ctcgactccc tggtcatgca attcggccgc
     2101 catgtcgcgg tcgagccggg gttccagatc tatggcacgc ccggcgcgat cggccatttc
     2161 gcccgctggg gccagcagga cgagcgcgcc gggccgcaac tcttcggcaa gatcttcaac
     2221 atcgggccgg gcacgctcga gtggaatggc ggcgtcctct tcggcctcag cccggccgtg
     2281 cccgcggtga cgccgcgctg gcagatcgaa tatgagatcc actactgatc gagaaaaccg
     2341 cgggcccggt cctctaccgc cagtcgtgat gatgccagcc gtcgccgtgg ccgtcttcga
     2401 cgaagcagcc ggtaagactg ccggcgatgc cgatgcccat cagcacgaga acgacggcgg
     2461 ttttcacttt gctcattggt tcgctccgcg agttgtccag ctatcggtac caacatcgcg
     2521 gggggagcgg atgatcccgg caagcggcgg ctcgaaggtt cgcagcctca acaacccgac
     2581 tatgcttctt cagatccggg acagcgtcac cgcgcatgcc atatgtcgca gcgtccggcg
     2641 aagatgcagg atgagccctc cggcaaaggc gatctcagcg gccatcgaca ggaggggcac
     2701 cagcgcggat ggcggcgccg cggccgacag ccgcgttgcg gtcaagacgg cgtcgctcaa
     2761 gaccgaagtg atcaccgcgg ccacggcaac ggcgcaaagg ccgaagtagc gacggagctc
     2821 gcggtcgtaa gcgcgatgaa atcgggccgg aacgcgccga cacgcaatgt agaccgcgcc
     2881 gagtacggcg ctgacaagag cgagcttgaa cgcataaccg ctgataggaa gcgcaagcgc
     2941 gcgaatcctc tgcacgtcct cggcgaactc aagaggcgcc ggcccgtaat atgccatgcg
     3001 cagcttcatg acgcagaaga tcccggcgac aacgagcagc gcgacgaggc cgttgcgccg
     3061 cgtctggcgg aagagcgacg cggcgacata gccctgcgcg atgtcgctag catcgccgaa
     3121 cctggcgagc gcgcgatgtt gcgcctcgac cgacggctct cgcccgtcgg ccgccgcggc
     3181 cgctgcttcc aagaggtgat cctcgacctc gcaacggaca caccgtgcga gcgcggcatc
     3241 gaagctcagc gcgcgggaga gcgcgtcgag gtagtcagcg accggactcg atgttggcat
     3301 ggccgagatc cccaagaagg ccaccaatgg cttcggtaaa gcgccgccat acggcgcggc
     3361 gatcggcgag tgcccgccgc ccgcgcggcg tcaacgcgta gacgcgtcgt tgccgcccgg
     3421 attccgcagt gatccagcgg ctcgcgagca agcccgtctg ctccaatcga tggagcaccg
     3481 gatagatcgt gccctccggc agatcgaagg cgccgccgct gcgccgatga atttcctgga
     3541 tcacggcata gccatgcgcc ggacccgcct gaagagcggc gagcacgatc atgtccatat
     3601 gccccttgag tatctcgccg ccttccatcg ccttgacgtc tcttcgtgtc gttatgtgat
     3661 gctatgccta gcgttacctt gggtcaaggt gtgcacatcg ccgacgagaa cggcgccatg
     3721 gaatggacga atgggcggcg aagcgcgctc ggaaaagcaa ttccgacacc cgggtggtca
     3781 gagcgccggg atcggtggtt gcggggacac gacttgaacc ctcgaccttc agtcgcgggt
     3841 cttcgactta gaacatgccg ttgctgttcg cggcctgctc ggggatgggc tgaacgacgt
     3901 cccagtgctc gacgatcttg cctttctcga gcttgaagat gtcgacgatg gcggagccgc
     3961 ggctgccggg ctcgcgcatc gcatggacgt gcaggatcac gtaatcgccg tcggcgaaga
     4021 cctgcttgat ctcactgtgg gagttcggaa atttctcgcg caggaaatcg aggaagtgct
     4081 tgaacccctc gggcccgtcg gcggcattgg gattgtgctg cgtgtagcgc ggcccgaaat
     4141 attgcgcggc cttggcgaaa tccttctggt tgatgccggc ctcgtagaat tcgaggaccg
     4201 tcttcttgtt ggtttcctgc tccgccgtgt cggcccgggc ggccggcccg aacagcacga
     4261 gcgccgtggc aaagcataga atgcgataca tgcgtcttcc ctcacgctgg cccaggttgc
     4321 ggcgagttta acccggattt cgcacatcag caccctccgg tagggaaggg cgagtctgcc
     4381 ccttgcccgg ccgaccctct tcaccatctt cagccggcgt cggggcggcg atggcgatct
     4441 ttgccgcccg cggtgtagaa taggcggcaa tgagcgagat ccagaaccag cgcgatatca
     4501 tcgaccgccg cgccgtctcg gcggcgctcg acgcgctggc cgacggcggt gccgagccgc
     4561 cccggacgaa agtcgtcgag gtcctgcgcg gggcactgca gcaggggcgc gccgaggtgc
     4621 gccggcgctt cgacgagcgc ggcaagggcg gcgccaccgt ccgcgagctc agcttcctca
     4681 tggatcagct cgtacgcgcg ctctacgatt tcgtcggcgc ccatgtctat ccgctggcca
     4741 acccgaccga gggcgagcgg ctcgccgtcg tggcggtggg ggggtatggc cgcggcgagc
     4801 tcgcgcctta ttccgacatc gatctcctgt tcctcttgcc ctacaagccg acgccgcata
     4861 ccgagcaggt cgtcgaatac atgctctatg cgatgtggga tctcggcgtc aaagtgggtc
     4921 aggcgacgcg ctcggtcgaa gagacgctgc gccagtccaa gaccgacctc accatccgca
     4981 ccgccgttct cgaggcgcgc tacatctggg gcgatgagaa gctgttcctg gagctgaagc
     5041 gccgcttcga cgccgagatc atcaagggca ccgccgccga attcgtgcag cagaagctcg
     5101 ccgagcgcga ccagcgccat gcgcgcatgg gcgacagccg ctacgtcgtc gagcccaacg
     5161 tcaaggaagg caagggcggg ttgcgcgacc tgcacacgct cttctggatc gccaaataca
     5221 tctatcgcgt cgacgacgtc gagaagctgg tggatctgaa cgtgctctcg gccaaggagg
     5281 cgcaccgctt catgcgggcg caagacttcc tctggaccgt gcgctgtcat ctccatttcc
     5341 tgaccgagcg cgccgaggat cgcttgacct tcgaccggca gaccgaggtc gggcggcgca
     5401 tgcgctatac cgaccatgcc ggcacgcgcg gcgtcgagcg cttcatgaag cactacttcc
     5461 tcatcgccaa ggacgtcggc gacctgacgc gcatcttctg cgctcagctc gaggccgagc
     5521 aggagtaccg gccccgcttc tcctggcgcc cctggagcgg gcgcaagcgt ttgatcgagg
     5581 gcttcgccgt cgatgccggc cgcctccagg tcgcgcgcga gagcacgttc cgcgacgacc
     5641 cggtgaacct catccgcctt ttccatgtgg cgcaggagca cgggctcgac attcacccgc
     5701 gcgcgctccg cctcgtcacc cagtcgctga agctgatcga cgccgatctc cgcgccaatc
     5761 ccgaagcgaa ccgcctcttc atggagattc tgacctcgcg caacgagccc gagatcgcgc
     5821 tccggcgcat gaacgaggcc ggcgtcttcg gccgcttcgt gccggatttc ggccgcgtcg
     5881 tcgcgcagat gcaatacgac atgtaccatg tctacacggt cgacgagcat tcgctcttcg
     5941 ccatcggcat cctgcacaag atcgaggcgg gcgcgctcaa ggacgagctg ccgctggcct
     6001 ccgacatcgt ccgcaccatc gattcgcgcc gcgcgctcta tctcgccgtg ctgctccacg
     6061 acatcgccaa gggaaggggc ggcgatcatt cagagctggg cgcccaagtg gcggagagac
     6121 tcgggccgcg cctcgggctt gccgccgacg agaccgagac cgtggcatgg ctggtgcgct
     6181 tccacctcct catgagcaac accgccttca agcgcgacat cgacgatccc cagaccattc
     6241 gcgacttcgt cgagcgcgtc cagtcgccgg agcggctgaa gctgctgctg atcctcaccg
     6301 ccgccgacat ccgcgcggtc ggtccccgcg tgtggaacgg ctggaaggcg acgctgctgc
     6361 gcgagctcta tcaccaggca atcgaggaaa tgtcgggcgg cctcgtcgcc gacagccgcg
     6421 atgcgcgcat cgccgcggcg cagaatgccg cgcgcgcgct cctgcccgac ttcaccgccg
     6481 aggagatggc cgccttcatc gccaagggct atccgtccta ctggctctct ttcgattccg
     6541 ccacccatgc ccgccatgcg cgtttgacgc gcgaggccga gcgcaccaac gcgccgctca
     6601 ccgtcgacac gagggtcgat acccggcgcg cggtgaccga ggtgacgctc tacaccgccg
     6661 accatgccgg actcttctcg cgcatcgccg gcgccttggc actcgccggc gccaacatcg
     6721 tcgacgccaa gatcctgacg atgtcgaacg gcatggcgct cgacactttc tgggtccagg
     6781 accagagcgg cggcgccttc gaccgcggcg acaagctggc gcggctcgcg gtgatgttcg
     6841 agaacgtgct gtcggggcgc accaagccgc atctcgaact tgccaagccc gatccgatca
     6901 tgagccgcac gcgcgtcttc accgtggcgc cgcgcgtgct gatcgacaac ggcgccagct
     6961 tgacgcacac cgtgatcgag gtgaacggtc gcgaccggcc cggtctcctc ttcgaggtga
     7021 cgcgcgcgct caccgccctc aatctgcaga tctcgagcgc caagatctcg acctatggcg
     7081 agaaggtcgt cgacgtcttc tatgtgaagg acctgttcgg ccacaaggtc gagcatgagc
     7141 gcaggctcaa ggacatccgc gagcatctgg tggcggtgct ggcggagccc gcggcagccg
     7201 ctgcgcgcgc ccgcgcgaag accatcgcgg cggaatgacg ttgccgcgcg ccggtcgacc
     7261 gacgcgcgtt ggcgcgtggc tggccaatcc ggtgagaacc gactagcatg cgatttctcg
     7321 ggaggcagct tgagcgccac ccgacaagcc gcgtgagacg gcggttctcg gaatggcctt
     7381 tgtcatttga tttcggtcgc cgtcggacat ctggcttctt cgaaaagttc agcaggggag
     7441 gatctcgaga tgcttagatc tctttcgacg cgagtagcga tgacgtcggc ggctgtcgta
     7501 ttgcttggcc tcgtcgtcaa tgccggccac gccgccggac cgttcgacgg gacctggcaa
     7561 ttggacgcgc ccggcgccgg cggcagcagt ccggaagccg gcagcaaatg cccggcggtt
     7621 cgccttcgct tcgagatcaa ggacaatcag gtggaggggc gttttgagcg ctcgcccgac
     7681 ctgcgccatg tcgagaccgg cgagggtcac gagggcacac ccgtcaccgg aagcgtcggc
     7741 cccgacggca cgctgaccac gaagtgggaa ggcatcaacg gcagcgggaa actggcgggc
     7801 aacgggggcc agctcaagtg gataggcgac tgcgggccgc gggtcggtac cctggcgcgc
     7861 gtcagctgac gtctggggct cgggcgcagc cggatccgcg agcatctggc ggcggtgctc
     7921 gcggaggccg cggcaacagc tccgcgcgcc cgcgcgaagt cagtggtaac agaataggcg
     7981 acagcccttg tctgttgtag taattccagt tgaagtatta gcgtcaaata agtaatactg
     8041 acgtcagcct gagacaaaat ttcggaggga cgtcatgaga cttgctaaat gggtgttccg
     8101 cgtcgcgggg gtctatggca tcgtcatgtt ggcgccggca tattttcttg agacgacttt
     8161 caccgattcg ctgcatcccg agttttatta tggtttcctt ggcgtgggac tggccttcca
     8221 agccctgttc ctgatgctgg cgagcgatcc aagccgctat cgcccgatga tgattcccgc
     8281 gatcctcgag aaggtcagct atggcgtcgc gaccttctgg ctgttcttcg ccgatcgcgc
     8341 cgacagcagt gtgctgctcg gcggctgcgt cgactggctg ttcgtcggct tgttcgcctg
     8401 ggcctatctg gcgacggcgc cttcgcaaga agtgcagcgc ggattgcgcg tccgggcggc
     8461 gtgaggattc ggaggaacta agaattagag aaagcggagg gcgctgagga tccgcggagg
     8521 acgcagagga gaatcgctcc tcagcgtcct tcgcgaaacc tctgcgacct ctgcgtttcc
     8581 tctttctttg cgtaggcgtc agcggagcga gtccccggcg agaccgcggc gcagccggcc
     8641 gagccaggtc gcgaaggaac gggcgaagaa tttcgtccaa tggttgccat ccccgacggc
     8701 aacgtcgaag gtcgcccacc attcgacaaa agctctgttg ccgtccacga tcggtgttac
     8761 ccggatcgtc gccacatagt cacgcacggg aaagtggcag ggctcgcaga attcgtaggt
     8821 gtagctgcgc tcccggtccg aatgtgcaag cagcttttga cgcgtgcgtg tctcacccat
     8881 ccggacgctg cggaccgcgc ccacggcgtc gcccggtctt tcatcctcga tcagcgcgtc
     8941 atcgacgcca tcgacccaga gcgcgtaatt tccgaaatcg cggatgacgg accagaccat
     9001 gccggcaggc tcatcgagca ccgtgctgta ataggatcgt gccatggctt cctcccttgg
     9061 tcgcgaggga ggaaatagcc gtcgccggag ctcgccgcgc tctgcttctt gctctcgaaa
     9121 ccgacatatc gtcggaaagc ccggtttctg ctcacgcggc cctgatctgg ccgatcacgc
     9181 gcaccaattc ctgcgacagc gcctcggatt gattggcgag ctcctgcgcc gcgctcaaca
     9241 cttcgcccga tgcgccggcg gtccgcatca cggcctcgcg aatgcccgtg acgttgttgc
     9301 cggccgcctc ggtgccccgc gacgcctcct cggcgttgcg ggagatttct cgcgtcgccg
     9361 cctgctgctc ctcgattgcg gcggagatcg tcgagacggt cgtgctgatg tcgccgatgg
     9421 tgccggcgat cgcttcgatc gcgccgaccg cctcggtggt cgccgtctga atctgggtga
     9481 tctggccgcc gatgtcgtcg atcgcccggg cggtctgatt cgccaatgcc ttgacctccg
     9541 aggcaacgac ggcgaatccc ctgccggcat cgcctgcgcg cgccgcctcg atcgtcgcgt
     9601 tgagcgcgag gagactcgtc tggccggcga tctcgttgat cagctcgacg acgcggccga
     9661 tcttctgcgc gcttgcggac agctcgtgga cgatcgcgtc ggtccgcttc gcgtcgccca
     9721 ccgcccggcc ggcaatcgcc gttgattgct cgacctgccg gccgatctcg tggatcgagg
     9781 aggtgagctg cgccgccgag gtcgccacgg tctcgacatt gacggcggcc tggccgacgg
     9841 cactgctgat cgcgagggac tggcggtcgc tctgcgccgc ggccgtcgac atggtttccg
     9901 ccgtccgctt gaggcgctgc gatgccgccg acaggctctc ggtgaggctg ccgatcttgc
     9961 cgtgcagctg gtcggcgatc gactcgagga agcggcggcg ctcggccgct gccgccgcct
    10021 cgctgttctg gcgctgcgtt tcgaggcgtt gcttctcgag ggcatttgtc ttgaacacgg
    10081 cgaccgagcg caccatgcgc ccgacttcgt cgcgccgtcc ggtgtcggca atctcgatcg
    10141 agagattgcc gctggcgagc atcgtcatgt cgcgatcgag ccgccgcaac ggcgtggcga
    10201 tgccgcgcgc gacgagccag acgatcagcg acgcgacggc gccgaccgcg gccgcgatgg
    10261 cgatgaagtg catgagcgtg ttgcgcacgc tggcgcccag atcgtccatg aagacgccgg
    10321 tgccgatcca catgtcccag gggatctctg cgacgtagtt gaccttctcg accggctccg
    10381 tctcgcccgg ccgggtccaa tagacggtgt agggcccgcc gcctttctgc gcgagctcgg
    10441 cctgatcgcg gatgatgagt cggccggact tgtccttgaa gtcgatgagg ttcttgccct
    10501 cgttctcggg atccgacgcc cgcgcaacga tcgtcccgtc catggtgtag acgaagtagt
    10561 agtccttatg cccgtaaaag gcatggtcga tctggcggcg gaagagctcg atcgcctcgg
    10621 gcttcgtgag cttgcccgcc ttgacgtcgg cgtcgagcga ttcggcgaag ctgcgcgcga
    10681 catcgatcgc cgcgtggagc tcggtgatgc gatcctcgcg catccggtcg tattgcgtga
    10741 tgaccgcaac gctggccatt accgcaagcg ccgccagggt gagcacgacg agcgactgaa
    10801 gcttgatcga cagcggcaga tcgcggagcc cgcgcctgcg gcttttcccg tccgtggttc
    10861 gcatcactca ctctccccgg ccagcctgcg acggctggcc ctcccggccc agacatacaa
    10921 gagcaggaaa cgggttaaga aaaggtgaga gcaacagcgt aatttatcga ttgctcaatc
    10981 atgcaacttc tacgtgacaa gagcgcgcgg ttgcgactac tccggcaaat ccgcgaggaa
    11041 ctccgccgca atcgtcttcg ccattctcac gagcgggacg ccgccatcct cgaggcgctc
    11101 cagcaccgtg aacccgtagg cgcgataaag cggctcgccc gacaacgtcg cgacgagctc
    11161 gaggcgggag aagccttcgc gcgccgctgc ttcctccgag agcctgagga tcaggcggcc
    11221 gacgccgcgg cgcacccagg acggatgcgt atacatcgcg cgcactcgtg cggcgtccac
    11281 ggccgggtcg agcagcgccg cgttcctgcc ggcgctgtgg tcgccgccat agatggtcgc
    11341 gcggcggctc cagccgccgc agccggcgac cgcagcgccg ccatagacga tccaataggt
    11401 gcgatcgctg atgagggtcg tatcgaggcc catgatggcg tggctggcgg cgatgagctc
    11461 gggcgtcaga aacggcttga ggttctcggc aatcgcggcg tccatcaccg cgcgcagaac
    11521 cggcgcgtcc gcttccaccg ccagccgtgc cgccaattct ttgcgcatgc cgcgcgtcgc
    11581 atccccgcgc cggctcggcc gtcgagccgc ggcgttttgt atcgctccca tggccaaagt
    11641 atagacggcg gcgcctcatg cccgccatgc gcagcccggc cgcccctcgc catggaccgc
    11701 cgccgatgcg acaggcctgc ccggcttgcg gcgagccgat cgccttctgg gcgcctgagc
    11761 ccggccggac cgcgcgcttc acttgcgcgg cctgcggcgc cgccggcaaa gtgcgcgggc
    11821 gagcgcgccg cgccgcgccg ccgcccgttt cgttgaaccg gttctagcgg cagcaccgaa
    11881 gcagcgccgg cttggccgac accgttgccg agacgaggtt ttgcatcgct cgcgcgaccc
    11941 actatagatc gagcgtatgg atgttcagtt cctcggctcc ggcgatgcct tcggcagcgg
    12001 cggacgcttc aacacctgca ttcacgtggc gaccgccggc ggcgccttcc tgatcgactg
    12061 cggcgcttcg agcctcgtcg ccctgcgcaa gttcggcgtc gatcccaacg gcatccatac
    12121 ggtcttcgtg tcgcatctcc acggcgatca tttcggcggc ctgccgttcc tgttgctcga
    12181 cgcgcagttc tacagccggc gcaacgcgcc gctgacgctg gtcggaccgc ccggttttcg
    12241 cgagcggctg acgcaggcga tggaagtgtt cttcccgggc tcgtcgcgcg tccagcgcaa
    12301 gttcgccact gagatcgtcg agatcgaagc gggaacgacc tcggcgatca acggcgtcga
    12361 cgtcacggcc catctcgtcg cgcacgcctg cggcgcgccg cccctggcgc tgcgcctctc
    12421 ttgcgagagg aaggtaatcg cctacaccgg cgatacggaa tggacggaga ctctgctcga
    12481 gctcggccgc gacgccgacc tgttgatcgc ggaagcgctg ttcttcgcgc gcaagatcaa
    12541 gcaccatctg gactatgcga cattgaaggc gaatttggtc cgcatcggcg cgaagcgcgt
    12601 cgttctcacg catatgggcc cggagatgct ggagcaccgc gcggacgtgc cggaagacgt
    12661 ggcggaggac ggcaagatca tccggttgtg aggaccgtgc agcccgccat atccgcagat
    12721 ccgcgaatgg tcaggcggac ggcaacccct cagctgcaag cacttcgtcc ggcgacgccg
    12781 gtgtttcact gccgccgagt tggcggtagg cgatgcagag aaagctgctg gcaacggcga
    12841 tctcgagcag aaaccagacg ttggacaagg gcatgagggc aatcgcggca gagtgcgctg
    12901 ccgcgcggcc ggcatgcccg gcgtaggcgg tcaacagaaa cagcgccccc aaacgggcgg
    12961 cctcgaaggg agcggcgcag ccgatcagac cgagacacag ctttgcatcg tggccacggg
    13021 atcggtgcca agccgtcagc ggcagcgcgc caggctcgcc catggcgatc gccggaaagg
    13081 caagaagcag gcgggcgagc ggcgtggccg cgacgaatat cacggccact aagaaaaagg
    13141 atctcagcga ctccggcatg ccttcatgaa aaatcaccgc cccggccgcg atgagaaaca
    13201 aggcgaggaa ggcgcccacg ggacgccgcg caactttcca gagcaacaga ctgagcagcg
    13261 cgaatccgaa tccgacaccg gctgcgatgc cgtaactcag gacgaacaac cccagtccgc
    13321 cgctcacgag gagcgggatg gcgagcaaca cgaagaggca gtaagcgaag aagcggaggt
    13381 cgcgaaagag cagccggagc gcgccgacac cggacgtcac cgatcccgtc agcacataac
    13441 gatgccaagc cagcgcaaag gccgacacga aaaaaatgtg gagcaggaag ctgaccacgt
    13501 ccggcgtgtg cagcagcacg gccggcgcgg gcattgaagc cagtcgccgc gcaagccacg
    13561 gaaagaaggc gaccaccatc agaacatatg cgtcgggctt tgcagaggcg gtgaagagcc
    13621 ccgcaatgct gatccccagc caagggacac ccgccagcac gagaaagccg acgacattgt
    13681 tgaacacgaa gccataggcg ccagcgagcg tgcggccgag cggcaagtga cgcggcgcgc
    13741 tcgcaaggac ctcgggggct tgttctgccg tgggagtggt catcggatgc gtggccgatt
    13801 cccttgcgtt tcgagggcga aggatggtgc cgcggcattg cataaatttg tatgaacgcg
    13861 cggaaaactc ttcattaatt ccacctgggg catgcttccg cttcggctcg gagatcgacc
    13921 ggagccgcct ctcgcgaggt caaagatgac ggcggccggc ccaacgctca tcaacaccga
    13981 tttccggaag gcgatcgagc gcaacaagga caataccttg ctgctgatcg aggtgctggc
    14041 cgtgagcgcg gccgtcgtcg gctatgtttt cggctggggc ctggccgtga tccgggacat
    14101 ctgggccatg ccgcccgcag cgttgtcgcg gctcgatgtc gcctggatcc tgcgcgacct
    14161 ttttactgta ccgccgcgga gcgaggcgct gatcggggcc ggcacgatgc ttgccgccag
    14221 catcgtctgg ggagtggtga tgctctatgc cggcgcgcat atcctctcca gcttcgcccg
    14281 cacccgagcg gccaatccgg agaaggcgtc cgagcagctc ttcatcgacg tcgtcaagga
    14341 gatggcgctt gctgccgggc tgccggcgcc gcacgccctg gtgatcgaca cgttggcgct
    14401 caacgccttc accgtcgggt cctcgcccga gcacgccgtc atcgccgtca cctcgggctt
    14461 gctccaagcg tgctcgcgcg aggagctgca aggcgtcgtc gcccatgaga tgggccatat
    14521 cgccgactac gacatgcgct acgccaccct cgtcgccgcc gcggccagca cggtcgtgct
    14581 cgtttgtcat ctgctgtggg gcgcgcgcgg gacatcgatg tggcgcggcg gcggcacggc
    14641 ggcgacacgt gccggactca ccctggccgt cctgatcgtc atgctggccg tgtcgatcgt
    14701 cgcccccctc gccgcgcggc tggtgcagtt cgccatctcg cgccagcgcg aatatcttgc
    14761 ggacgccacc tcggtgaagc tgacgcgcga tcccgccggg ctcattcacg ccctgcaacg
    14821 cctcgagcag ggcaacaccg acctggcgca tgccggctcg ccgctgctcg ccctctgcat
    14881 cgccgcaccc aaaagcgagg tgcccggcga tctcctggcc tctcatccgc cgatcgccga
    14941 ccggatcgcg cgcctgcgca acctcggcga gatcgccgcg ccggccatcg cggcgagctc
    15001 aggatgacgc gcgatagctg cgccaaggaa cgcagaagaa agattcaacg gtgagaagag
    15061 tcctggcgcg cgaagcacgc tttggtttct tctcaccgtc tcactgtcta tccgcgccct
    15121 ccgcgctcct ctttcttttc acccgccgag atactgccgc agcagcgcgg tgctgcggcc
    15181 ccaggcatcg gcggtgt
//
LOCUS       DASYNS010000016         5984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4372568, whole genome shotgun sequence.
ACCESSION   DASYNS010000016 DASYNS010000000
VERSION     DASYNS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4372568"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..633
                     /locus_tag="VGP50_00600"
     CDS             295..633
                     /locus_tag="VGP50_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV8025891.1"
                     /translation="MARSTENVKNWMTMFRWIVKLMRDDYAIEESRLTRAAVLDRLGL
                     SHEQIEELMATIARSFGIRFPDHAFDEIVKLEELCLVASWLKGYYKRPDFISPHFERK
                     CRAANPSATV"
     gene            complement(643..1431)
                     /locus_tag="VGP50_00605"
     CDS             complement(643..1431)
                     /locus_tag="VGP50_00605"
                     /inference="COORDINATES: protein motif:HMM:NF039950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5984 family protein"
                     /protein_id="HEV8025892.1"
                     /translation="MSSASQSRSVHDPSSAATTAFKINFKLREIDHIEPWGEPGKRSL
                     HWFGLTDGAYCFDTSVGRLLEHTDAVDPDFGEPWCEYQVVRLFEDLTEIWPRVSEPVP
                     IDVVTRYLAWAAREGERFSNTDDPDLRETWYEMSSWWLDRRLDFSYLRPAPQFHLWRM
                     GADAHFEWRASAPWLPPVAAFSLPFESARNAVDSFFQEFVAAMDERVETIRRSGWQPV
                     DAMLDIPALVAEEKQRRKVAASPFSHLGETDWQLIRARLKELGA"
     gene            complement(1428..2099)
                     /locus_tag="VGP50_00610"
     CDS             complement(1428..2099)
                     /locus_tag="VGP50_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEV8025893.1"
                     /translation="MNSLTRRQMAWRAAQDIEDGAYVNLGIGLPTMVSDFIPRDREIV
                     FHSENGILGMGPAPKPGEEDKDLINAGKGLTTLVPGGAYFHHNDAFLMIRGGHLDLAL
                     LGAFEVSETGDLANWTTDDPHFAPGVGGAMDLAVGAKEVRAILEHVTKDGTPRIRKRC
                     VYPLTAPHCIKRIYTNLAVMDVTEDGLVVREMVEGIDFPRLQNVTEARLTLANDWRPL
                     AAPLV"
     gene            complement(2096..2782)
                     /locus_tag="VGP50_00615"
     CDS             complement(2096..2782)
                     /locus_tag="VGP50_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HEV8025894.1"
                     /translation="MIDKRMRSLAEAVQGVKSGATVLCSGFGAVGEPVELLNALRETG
                     VRDLVCVANNAGTGDEGLAGLIKEGRVRRIICSYPRTVDPHCFEAAYQAGKIELELVP
                     QGTLSERMRAGAAGIGGFYIKTSVDTRLAEGKELREIDGERYVLEKPIRGDVALCRAD
                     RADRWGNLTYRKSARNFNPVMAMAAALTVVQVREMVELGALDPECVATPGIFVDRVVV
                     VREGREGEKP"
     gene            complement(3162..4529)
                     /gene="pcaB"
                     /locus_tag="VGP50_00620"
     CDS             complement(3162..4529)
                     /gene="pcaB"
                     /locus_tag="VGP50_00620"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HEV8025895.1"
                     /translation="MTVNPADSRVFGTLFGTDEMRGVFGDRALVQKMLEVEAALARAE
                     ARLGIVPAAAAQAIAAAAAVERIDLKALGESTRQVGYPVVGLTKALARAAGGDAARYV
                     HWGATTQDILDTALVLQIREGLAAVERDLRALAEALAEKADAHRAAVMAGRTHLQHAL
                     PVTFGYKCAVWLAPLLDHLMRLAELRRRVLVVQFGGAVGTLASLGDKGRAVTEALAGE
                     LGLAAPVAPWHVSRERPVETAAFLALVCGTLAKIATDVILLMQSEIAEVLEPHATGRG
                     GSSTMPQKRNPIASEYILAAARGVHALLPLLFGAMAQDQERATGPWQSEQLALPQIFV
                     LTAGALAHGVTLARGMTVDAARMRANLAATDGLIMAEAFMMALAEKVGRDAAHHLVEK
                     ASNAARAAGRPLVETLGADPDVARHLDGAALARLADPAHYLGEALAVVDRVLAVSRER
                     LQSRV"
     gene            complement(4536..5114)
                     /gene="pcaG"
                     /locus_tag="VGP50_00625"
     CDS             complement(4536..5114)
                     /gene="pcaG"
                     /locus_tag="VGP50_00625"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEV8025896.1"
                     /translation="MRLIPTASQTAGPYFAIGLAHREWSDLTSGGKAAGPRIRIEGRV
                     FDGDKKPLDDALVEIWQANAAGRYAHPEDRQARPLDPHFRGFGRCATDKDGRYRFLTV
                     RPGPVPGRGNTLQAPHISVAVFARGLLKQATTRLYFPGEALNNADPVLALVDDARRAT
                     LIAADAGEESGTRFYRFDIVLQGEGETVFFDV"
BASE COUNT         1073 a         1991 c         1990 g          930 t
ORIGIN      
        1 ggggcgctgg ctgcgctcgc tgaaccgccg cgagctggag atcgccagtc gcctggtcgg
       61 cgtatcggcg gtaccgatcc ccgcccagtc cgcgccggcg aatgacctcg aatgcccctg
      121 cggctccggc aagagggccg aacactgcca cggcgccccg gcctagcgcc ctcccgactc
      181 gcggcacaaa gcggcgcaat ttccacattt cgcaagtact tgtgctgaaa aggcattagc
      241 gattgcgtaa cgggtcttaa gtatacttgg ccggtttgag tgacggcgga cccgatggcg
      301 cgatcaaccg aaaacgtcaa gaactggatg accatgttcc ggtggatcgt caagctcatg
      361 cgcgacgatt acgccatcga ggagtcgcgc ctgacgcgcg ccgcggtcct cgaccggctc
      421 ggcctctccc acgagcagat cgaagagctg atggcgacga tcgcgcggag cttcggcatc
      481 cgcttccccg accatgcgtt cgacgagatc gtgaaactcg aagagctgtg cctggtggcg
      541 agctggctca agggatatta caagcggccg gatttcatct cgccgcattt cgagcgcaaa
      601 tgccgcgccg ccaacccgtc ggccacggtc tgacctcact ggctacgcgc cgagttcttt
      661 cagtcgcgcc ctgataagct gccaatcggt ttcgccaagg tgtgaaaaag gcgacgctgc
      721 gactttcctt cgctgctttt cctcggcgac gagcgcagga atgtcgagca tggcatcgac
      781 cggctgccag ccgctcctcc tgatggtttc cacgcgctca tccatagcgg cgacaaactc
      841 ttggaagaag gagtccactg catttcgcgc gctctcaaat ggcagggaga acgcggcgac
      901 cggtggcagc catggcgcgg atgcgcgcca ctcgaagtga gcgtccgcgc ccatgcgcca
      961 aaggtggaat tggggcgcgg gtcgcagata cgagaaatcc agccgccgat cgagccacca
     1021 ggagctcatc tcgtaccatg tctcacgaag atcaggatca tcggtattcg agaaccgctc
     1081 gccctcacga gcggcccaag caagatatcg ggtcacgacg tcaatcggca ccggttcaga
     1141 gacgcggggc cagatctccg tgagatcctc aaacagccga acgacttgat actcgcacca
     1201 gggctccccg aagtcgggat cgacagcgtc cgtatgctcg agcagccgcc cgaccgaggt
     1261 gtcaaagcaa taggccccat ccgtcagacc gaaccaatgg agcgatcgct tccccggctc
     1321 gccccatggc tcgatatggt cgatctcgcg cagcttgaag ttgatcttaa atgccgtcgt
     1381 cgccgcgctg ctcgggtcat gtacggaacg cgattggctc gcgctactca cacaaggggc
     1441 gccgcgagcg ggcgccagtc attggcgagc gtcagccggg cttccgtcac gttctgcagg
     1501 cgcggaaaat cgatcccttc gaccatctcg cgcacgacca gcccgtcctc cgtcacatcc
     1561 atgacggcga ggttggtgta gatccgcttg atgcagtggg gggcggtcag cggatagacg
     1621 cagcgtttgc ggatgcgtgg cgtgccgtcc ttggtgacgt gctcgagaat ggcacgcacc
     1681 tctttggcgc cgaccgcgag atccatcgcg ccgccgacgc cgggcgcgaa atgcggatcg
     1741 tcggtcgtcc aattggcgag gtctcccgtc tccgaaacct cgaacgcgcc gagcagcgcc
     1801 aggtcgagat ggccgccgcg aatcatcagg aaggcatcgt tgtggtggaa gtaggcgccg
     1861 cccggcacga gggtcgtgag gcccttcccg gcgttgatca gatccttgtc ttcctcaccg
     1921 ggcttgggcg ccggccccat gccgaggatg ccgttctcgc tgtgaaagac gatctcgcga
     1981 tcgcgcggga tgaaatcgga gaccatcgtc ggcaggccga tgccgagatt cacataggcg
     2041 ccgtcctcga tgtcctgcgc ggcacgccaa gccatctgcc tgcgggtgag cgagttcatg
     2101 gcttctcccc ttcccttcct tctcggacga cgacgacgcg gtcgacgaag atgccgggcg
     2161 tcgccacgca ttccgggtcg agcgcgccga gctcgaccat ctcgcgcact tgcaccacgg
     2221 tgagcgccgc cgccatcgcc atcaccgggt tgaaattacg cgccgacttg cgataggtga
     2281 gattgcccca gcggtcggct cgatcggcac ggcagagcgc gacgtcgccg cggatcggct
     2341 tctccaggac atagcgctca ccgtcgatct cgcgcagctc cttgccctcg gcgagccgcg
     2401 tgtcgacgct ggtcttgatg tagaagccgc cgatcccggc ggcgccggcg cgcatgcgct
     2461 cggacagcgt gccctggggc accagctcga gctcgatctt gccggcctgg taggccgcct
     2521 caaagcaatg ggggtcaacg gtgcgcggat aggagcagat gatcctgcgc acgcggccct
     2581 ccttgatgag gccggccaag ccttcatcgc ccgtcccggc attgttggcg acgcagacga
     2641 ggtcgcgcac gccggtctcg cgcagggcgt tcaggagctc gaccggctcg cccaccgcgc
     2701 cgaagcccga gcacagcacg gtcgccccgc tcttcacccc ttggacggcc tcggcgaggc
     2761 ttcgcatgcg cttgtcgatc atcggctccc gttccccgac acgaaagaag cgatgaatgt
     2821 gctgaagcat gcaggtgttg gcaagcccgc ccggcgacga cgctgcgcgt cgcgccgaaa
     2881 agggaaccgc ccgccggcag gggggtgcgg gcgggcggtt gaagagccgt tgtgggggcg
     2941 cggcagcgat cgcggtctgt ctcgagcgct gccgtttcgg gggggaagcc accacagctt
     3001 cggggggaag tcatcacagc tcgtaagacc aacaatgccg ctttcggcgc cggcgcgaca
     3061 gtcggcgcca cggcttcgtc cgtccgggcg aggcggcgcc tccgccgatc gatcggatgg
     3121 cctaagcctg acccgaaacc gcggccggaa cggccgcacg ctcaaaccct tgattgcaag
     3181 cgctcgcgcg acaccgcgag aacgcgatca acgaccgcca gcgcctcgcc gaggtaatgc
     3241 gcgggatcgg cgaggcgcgc cagcgccgcg ccatcgagat gccgcgcgac atcgggatcg
     3301 gcacccagcg tctcgaccag cgggcgcccg gccgctcgcg cggcgttgct cgccttctcg
     3361 accaagtgat gggcggcgtc acggcccacc ttctcggcaa gcgccatcat gaaggcctcg
     3421 gccatgatca gcccgtccgt cgcggcgaga ttggcgcgca tccgcgcggc atcgacggtc
     3481 atgccccgcg ccagggtcac gccatgggcg agcgcgccgg ccgtgagcac gaagatctgc
     3541 ggcagcgcca gctgctcgct ctgccagggg ccggtcgcgc gctcctggtc ctgcgccatc
     3601 gccccgaaga gcagcggcag cagcgcatgg acgccgcgcg cggcggcaag gatgtattcg
     3661 ctggcgatcg ggttgcgctt ctgcggcatg gtgctggagc cgccgcggcc cgtcgcatgg
     3721 ggctcgagca cctcggcgat ctcgctctgc atcagcagaa tgacgtcggt ggcgatcttg
     3781 gcgagagtgc cgcagacgag cgcgaggaaa gcggccgtct cgaccggccg ctcgcggctc
     3841 acatgccacg gcgccaccgg cgcggcgagg ccgagctcgc ctgcgagcgc ctccgtgacg
     3901 gcgcggcctt tgtcgccgag cgaggccagc gtaccgacgg cgccgccgaa ctgcaccacc
     3961 agcacgcggc ggcgcagctc ggccaagcgc atgaggtggt cgagcagagg cgcgagccac
     4021 accgcgcatt tgtagccgaa ggtgacgggg agcgcgtgct gaagatgcgt gcgcccggcc
     4081 atgacggcgg cgcgatgggc gtcggccttc tcggccagcg cctctgcgag cgcgcggaga
     4141 tcgcgctcga cggcggcgag cccttcgcgg atctgcagca cgagggcggt gtcgagaatg
     4201 tcctgcgtgg tggcacccca atgaacatag cgcgcagcat cgccgccggc cgcgcgggcg
     4261 agcgccttgg tgaggccgac cacgggatat cccacctggc gcgtgctctc gccgagcgcc
     4321 ttgaggtcga tgcgctcgac cgcggcggcc gccgcgatgg cctgtgccgc ggccgcgggg
     4381 acgatgccga gccgcgcctc ggcgcgcgcc agcgccgcct cgacctccag catcttctgc
     4441 acgagcgcgc gatcgccgaa gacgccacgc atctcgtcgg tgccgaagag cgtgccgaag
     4501 acccgcgaat ccgccggatt gacggtcatg ggcgctcaca catcgaagaa caccgtctcg
     4561 ccttcgcctt ggagcacgat gtcgaagcga tagaatcggg tgccgctctc ctcgccggca
     4621 tcggcggcga tcagcgtcgc gcgccgcgca tcgtcgacga gggcgagcac cgggtcagcg
     4681 ttgttgagcg cctcgccggg gaaatagagc cgcgtcgtcg cttgcttcag gagtccgcgg
     4741 gcgaagaccg caacgctgat atgcggcgcc tgcagcgtgt tgccgcgtcc cggcaccgga
     4801 ccgggccgca cggtgagaaa gcggtaacgg ccatccttgt cggtggcgca gcggccgaag
     4861 ccacggaaat gcggatccaa gggcctggcc tggcgatcct cgggatgggc atagcgaccc
     4921 gcggcattgg cctgccagat ctcgacaagc gcgtcgtcga gcggcttctt gtcgccgtcg
     4981 aacacgcggc cctcgatgcg gatgcgcggg cccgccgcct tgccgccact ggtgagatcg
     5041 ctccattcgc gatgagcgag gccgatcgcg aagtaggggc ctgcagtctg cgacgcggtc
     5101 gggatgaggc gcatgggatt gacctctatg gtgacgagaa ccgcgtcatc cttgccaaca
     5161 ggaaggcgga cgcggttagc aggactgcaa agatggccgt cgtgtcgacg acgaaccgga
     5221 atagtatccc cgatacacca gccccgcttg ttatgtcaga cgatccccca taggcaagga
     5281 acccggccgc cgccagggat acaaaggtga gcgatgtgag aaggatgtat cgccaggcct
     5341 catcaccgac tgcttgatcg gaagatcgtt ccgtcggaac gcgcaaccag cgtacataga
     5401 cggcgacgag gaccgttgcg ccgagcagcg tgctggcatg ctgcagcacg ttatagagat
     5461 gaaactctct gccgatcatg ctgaagaccg acaggcgcaa gactgatgag tgcgagacca
     5521 cgtagccact ctcatgcgtg aatgagtccc ataggatatg tgtaaccgca cctaattagc
     5581 gcgaccgaca gcgatacgcg caccattgta gtaagtgtcg tgagcgcagg cgctagcggc
     5641 aaggaaagca gcgctccgcg atgtggttgt ggcatcagcc aagcgaccgg cctgtgcccg
     5701 acacgaagca gcgcgacgag aacgagccca gccggcaggc agaccgtcag cagaccaagc
     5761 ggcctatgcg cggctgaggc gagatcaaag cgaccgacat aatagccgat gtcgggcacc
     5821 atgcttcccg caaccaagcc cgcaaaggaa agcggccctg cacgacgcag gaacacgacg
     5881 gctgctggat gcgcgaatgt ccagggcacg actcagctcg atctgcgcgc ggcaagacgt
     5941 cccctctccc cctgcgggag agggaggggc ccgacgcgca gcgt
//
LOCUS       DASYNS010000017         4025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5152071, whole genome shotgun sequence.
ACCESSION   DASYNS010000017 DASYNS010000000
VERSION     DASYNS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5152071"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..1564
                     /locus_tag="VGP50_00630"
     CDS             365..1564
                     /locus_tag="VGP50_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8025897.1"
                     /translation="MGRKLSIAALGAALAGGLFLRPAAAEPIKIGIILTYSGPNASLG
                     DQIDKGLRLYVKEHEKDLPPGTTVELVRRDDTGPNPDVARRLAQELVTRDHVQFLTGV
                     VWSPNAGAIAPIASEAKVPFIISNAAAAGLTRASPEIARVSFTLWQESLPLGTWAAQK
                     GGMKKVYIAVSDFAPGKDAGDAFTKGFTDAGGQIVDIVRFPLKDPDFVPFMQRIKDAK
                     PDAVFVFVPSGKQATAVMKAYNELNMKGDGIKIIGPQDIVPDEELPSMGQEPLGVISA
                     GTYSNAGDRPANKAYVAAWKREYGDETPPGFMSVGGWDSMAAIFSVIKAMNGKVDGDK
                     PMALLAGWKDPDSPRGPIMIDPQTRDIVQNVYIRRVEKHGDRLANIEFETIPQVKDPW
                     KELNPPK"
     gene            1612..2475
                     /locus_tag="VGP50_00635"
     CDS             1612..2475
                     /locus_tag="VGP50_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8025898.1"
                     /translation="MSAAFVSIAFHGVAFAMVLYLMAVGLSVTMGLMGFVNLAHGVFA
                     GAGGYVMTSLMNVYHVPFPLAVVAAFVAVALGSVILERLLYRRFYGGAELDQVLLTVG
                     LVLMSVAIAHYFWGPLAQPVRMPSYLSGQIHLGIGTFPIYRSFIILAGAALVTLLWLG
                     LERTRFGARIRAAVDNRRMAQSVGIDTGRLFTLTFALGSGLAALGGALSAEFWAIDPG
                     YALEHLVYFLIVVAVGGLGSIKGPFFAALLLGIADIACKYLVPEFGAFFIYALTLAIL
                     LWRPQGLFGRA"
     gene            2475..3473
                     /locus_tag="VGP50_00640"
     CDS             2475..3473
                     /locus_tag="VGP50_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8025899.1"
                     /translation="MAETPEARAAPAVEALLRRHRLRLVEALPWLAAAVCYVAFPDYL
                     PLGSQIIINILFALSLDLILGYAGIVTLGHAAFFGAGAYAAGLLAAHGWNEPLSGLVV
                     AGVAAGAVGLASGAVILRTRGLTLLMLTLVIGEMLSEAANKAGAITGGADGLQGMIVA
                     PVLGFFRFDLYGRTAYLYCLAVLFLGWLAARQIAHSPFGRSLIGIRENEARMHAIGSP
                     VFRRRLTIYTISAALAGIAGALLAQTTQFVALEVLSFGRSGQVLIMLILGGVGRLYGA
                     FIGVPLYMVLQDRFAQADPVYWNFWIGLGLVLVVVFSPGGVLGQLVPLAQRLGRKP"
     gene            3470..>4025
                     /locus_tag="VGP50_00645"
     CDS             3470..>4025
                     /locus_tag="VGP50_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8025900.1"
                     /translation="MTPALETKGLCKSFGALTVANDIDFSLERGARHALIGPNGAGKT
                     TFVNLVTGALAPSAGRILLDGSDITALPQARRVKRGLARTFQLNSLFRGLSVLENVCL
                     ALGERLSVAGDMHRPAGSRRDVVEGAYALLEQLLLADDAGRLVRELAYGRQRLVELAV
                     ALGLEPRVLLLDEPAAGVPSSESGI"
BASE COUNT          578 a         1390 c         1318 g          739 t
ORIGIN      
        1 catgctgcgc gagaagcgca atggcttcgc cgagcgaggc cgggcgatga tattcgaagc
       61 gccctggtat cacttgagct tcctcccttt tgctgcgccg ttcaaccgga cgtctttatt
      121 tacttccgcc aagtctccgg gagagtgcga cggctgtcaa tgaatcgatg gaggcgagcg
      181 gatcgggaca gggcacgcgt catgagtgca tgccgtcgct cgacgcgtga gagggtgccc
      241 ccgaacgcgc ggcgctttac aatcccgcgc ccgaacgttg ctccggtcgc ttggccgtcg
      301 ctatagtcgg cgtgaacagg cggcacagcc tgagaaacgg cacggcccga gggggtggac
      361 gatcatggga cggaaattgt cgatcgccgc tctgggggcg gcgttggccg gcgggctctt
      421 ccttcggccc gcggccgcgg aaccgatcaa gatcggcatc attctcactt attccggccc
      481 aaatgcctcg ctcggcgacc agatcgacaa ggggctccgc ctttacgtca aagagcacga
      541 gaaggatctg ccgcccggta ccacggtcga gctcgtccgg cgcgacgaca ccgggccaaa
      601 ccccgacgtc gccaggcgcc tcgcgcagga gctggtgacg cgggaccacg tgcagttcct
      661 gacgggcgtc gtctggtcgc ccaatgccgg cgcgatcgcc ccgatcgcct ccgaggccaa
      721 ggtgcctttc atcatcagca acgccgcggc ggcgggcctg acgcgcgctt cgcccgagat
      781 cgcccgcgtc tccttcaccc tctggcaaga gagcctgccg ctcggcacat gggcggcgca
      841 gaagggcggg atgaagaagg tctatatcgc ggtcagcgac tttgccccgg gcaaggacgc
      901 cggcgacgcc ttcaccaagg gcttcaccga tgccggcggc cagatcgtcg acatcgtgcg
      961 ctttccgctc aaggaccccg atttcgtgcc gttcatgcag cgcatcaagg acgccaagcc
     1021 cgatgcggtc ttcgtcttcg tgccttcggg caagcaggcg accgcggtga tgaaggccta
     1081 taacgagctc aacatgaaag gcgacggcat caagatcatt ggcccgcagg acatcgtccc
     1141 cgacgaggag ctgccgagca tgggccagga gccgctcggc gtgatctcgg ccggcaccta
     1201 ttccaatgcc ggcgaccgcc cggcgaacaa ggcctatgtg gcggcgtgga agcgcgaata
     1261 cggcgatgag acgccgcccg gattcatgtc ggtcggcggc tgggacagca tggcggcgat
     1321 cttctccgtc atcaaagcga tgaacggcaa ggtcgacggc gacaagccga tggctctcct
     1381 cgccggctgg aaggacccgg acagcccgcg cgggccgatc atgatcgatc cgcagacgcg
     1441 cgacatcgtg cagaacgtct atatccgccg cgtcgagaag cacggcgacc gtctggccaa
     1501 tatcgaattc gagacgattc ctcaggtcaa ggacccgtgg aaggagctca acccgccgaa
     1561 gtaacggcag cgcgccctct tccgctcgcc gagagggagt agtgtgggac tgtgagcgcc
     1621 gccttcgtca gcatcgcttt tcatggtgtc gccttcgcca tggttcttta cctgatggcg
     1681 gtggggcttt ccgtgaccat gggcctcatg ggcttcgtca acctcgcgca tggcgtgttc
     1741 gccggggccg ggggatatgt gatgacgagc ctcatgaacg tctatcacgt ccccttcccg
     1801 ttggcggtcg ttgcggcatt cgtcgcggtg gcccttggca gcgtcatcct cgagcggctg
     1861 ctctaccgcc gcttctacgg cggcgccgag ctcgaccagg tgctgctcac cgtcgggctc
     1921 gtgctgatgt ccgtcgccat cgcgcattat ttctggggac ccctggcgca gccggtgcgg
     1981 atgccgagct atctgagcgg ccagatccat ctcggcatcg gcacctttcc gatctatcgg
     2041 agcttcatca tcctggccgg cgccgcgctg gtgacgctcc tctggctcgg gctcgagcgc
     2101 acgcgcttcg gcgcccgcat ccgtgccgcc gtcgacaacc ggcgcatggc gcagtcggtc
     2161 ggcatcgaca ccggccgcct cttcaccctc actttcgcgc tcggcagcgg gctcgccgcg
     2221 ctgggcggcg cgctcagcgc cgagttctgg gccatcgatc cgggctacgc gctcgagcat
     2281 ctcgtctatt tcctcatcgt cgtcgcggtg ggcggtctcg gcagcatcaa gggtcctttc
     2341 ttcgccgcct tgctgctggg gatcgccgac atcgcctgca agtatctggt gccggagttc
     2401 ggcgcgttct tcatctatgc cctgacgctc gccatcttgc tctggcggcc gcagggcctg
     2461 ttcggccgcg cctgatggcc gaaacgccgg aggcgcgcgc ggctccggcg gtcgaggcgc
     2521 tgctccgccg ccatcgcctg cggctcgtcg aggccttgcc ctggctcgcc gcggcggttt
     2581 gctatgtcgc tttccccgac tatctgccgc tcggctcaca gatcatcatc aacatcctct
     2641 tcgcgctctc gctcgacctg atcctgggct acgccggcat cgtcaccctg ggccatgcgg
     2701 cgttcttcgg cgcgggcgcc tatgcggccg gactgctcgc ggcccatggc tggaacgagc
     2761 cgctgagcgg cctcgtcgtc gccggcgtgg cggcgggcgc cgtcggcctc gccagcggcg
     2821 ccgtcatcct gcgcacccgc ggcctcacac tcctgatgct gacgctggtc atcggcgaga
     2881 tgctgtccga ggcggcgaac aaggccggcg cgatcaccgg cggcgccgac gggctccagg
     2941 gcatgatcgt cgcgccggtg ctcggtttct tccgtttcga tctctacggc cgcaccgcgt
     3001 atctctactg cctcgcggtc ctattcctcg gctggctcgc ggcgcggcag atcgcgcatt
     3061 cgccctttgg ccgctcgctg atcgggattc gcgagaacga ggcgcggatg cacgccatcg
     3121 gctcgccggt cttccgccgc cgcctcacca tctacacgat atcggcggca ctggccggga
     3181 tcgccggcgc gctcctggcc cagaccacgc aattcgtggc gctcgaggta ctgagcttcg
     3241 gccgctcggg ccaggttctc atcatgctca tcctcggcgg cgtcgggcgg ctctatggcg
     3301 ctttcatcgg cgtgccactc tacatggtgc tgcaggatcg cttcgcgcaa gcggatcccg
     3361 tctactggaa tttctggatc ggtctcggac tggtgctggt cgtcgtgttc tccccgggcg
     3421 gcgtgctcgg tcagctcgtg ccgctcgcgc aacggcttgg ccgcaagccg tgacgcccgc
     3481 tctcgaaacg aaagggctct gcaagagctt cggcgcgctg acggtggcga acgacatcga
     3541 tttcagtctc gagcgcggcg cgcgtcacgc gctcatcggt cccaacggcg ccggcaagac
     3601 gaccttcgtc aatctcgtga cgggagcgct cgcgccgagc gccgggcgca tcctgctcga
     3661 cggcagcgac atcaccgcgc tgccgcaggc ccggcgggtc aagcgcggcc tcgcccgcac
     3721 cttccagctc aacagcctct tccgcggcct ctcggtgctg gagaatgtct gccttgcgct
     3781 cggcgagcgg ctgagcgtcg ccggcgacat gcaccgcccg gccgggtcga ggcgcgacgt
     3841 ggtcgagggc gcttatgcgc tgctcgagca gctgcttctc gccgatgacg ccggtcgtct
     3901 cgtccgcgag ctggcctatg ggcgccagcg cctggtcgag ctcgccgtgg cgctcgggct
     3961 cgagcccagg gtgctgctgc tcgacgagcc ggccgccggc gttccgtcgt cggaaagcgg
     4021 catca
//
LOCUS       DASYNS010000018         5822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1408092, whole genome shotgun sequence.
ACCESSION   DASYNS010000018 DASYNS010000000
VERSION     DASYNS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408092"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VGP50_00650"
     CDS             <1..703
                     /locus_tag="VGP50_00650"
                     /inference="COORDINATES: protein motif:HMM:NF028481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEV8025901.1"
                     /translation="YVFGPNFGLPDPSPFVLKTTVQTKMTGLPYRTAIGQLPEAPKGK
                     LPYIVDGDVTLGDSTFIRAYLEHKSGTDLDHGLDARERALGWAVERMLEDHLYWAILE
                     HRWLDADNFARGPAHFFDGAPEAVRDKLRAEAAGRVRERLHGHGLGRHSPAEIADLGA
                     RSLASLGALLGERPYLFGAEPKAADATAFALVAAAAVPLFTGPLADAAQSDRRLMAYR
                     DRMLQRFFADFSAAA"
     gene            1085..2710
                     /locus_tag="VGP50_00655"
     CDS             1085..2710
                     /locus_tag="VGP50_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEV8025902.1"
                     /translation="MALAATVALPHRASAVPPARYRRWNVSDPDCPSRVLQSYKKAIR
                     AMLALPPGDPRNWYRYTLIHALDCAHGNWWFLPWHRAYIGWFERICRELSGDPEFALP
                     YWDWTREPRVPKAMFEDVLDPNDRAFIATSGAFKAAYQDAVAAAGYWRMTRNAELDLR
                     PSPQYAGLLERSIRSPEDLWFNIIADPAGPMFFDQPHARGLSASAPELTVPDAPPERQ
                     PVLVAVAAATLGDALAPRDFTSFAGPKVPAHSVLSGFGVLESQPHSLVHECLGGGYNG
                     AGGFMRGLMSPVDPIFYLHHTNIDRLWDIWARKQRAAGDPALPEGYSDSTGRKAAGDS
                     DFAAWAREPFLFFVDTKGDPVRQSTAGAYAEIGDFDYDYAPGSGEEVVPATASPAETA
                     AAPRKTLPVRLVAGRIAESSPARGSVVVPWALVAQQAEPQQPRLFASVTISVPPHRHT
                     AFDVLLAGAAGAPPIFVASLLTFGHHGMAGPVSFRVPLSAALAKAQANHTSGDDLTLD
                     ILVAQRAGSRGMAAMAGPSPGASEVTAMSLDQL"
     gene            2730..3227
                     /locus_tag="VGP50_00660"
     CDS             2730..3227
                     /locus_tag="VGP50_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025903.1"
                     /translation="MMRRLSAIALLSALVSAAALAQTRLPSDAAILAGKWTYRSYVNS
                     PALVERDKDKALAIIFGEGTYAFDPPSGAAITGTLDLGGGRVLDLKGSITQTAPLSLA
                     IAGYGRAGTPTEGWEYDYNASLAYEWPAGVDQRPALVGSVIRAKPHGSGPAGVVASFI
                     AVKQP"
     gene            complement(3234..4175)
                     /locus_tag="VGP50_00665"
     CDS             complement(3234..4175)
                     /locus_tag="VGP50_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025904.1"
                     /translation="MPQAPDCSTHFLFEHNVFKVDGGRFAFTADGTEPAFHIALGSLA
                     AALRLPGLRKEFAIDAASTDGRLLRIVELSLRFVKEIRPGDSIPREILDGTASWSVSD
                     GHRATARGRLIAHAVTAVDGKPADTEISMLRWLGASENGTRQLEQAAAGIAERLGVDA
                     GKAEILARIDRLARELCYIEALRERCALLRVVAAKINVLAKLYRGDRTILDNISRVST
                     LLQRPYGEFEHAFDRLDAATSEILTVLLGIDDHVQQIRRARDDVHMRLMPWDDVIAIW
                     TALDPVRGTETEAALDEFYRFAARHYLVQTEQRAGLL"
     gene            complement(4261..4464)
                     /locus_tag="VGP50_00670"
     CDS             complement(4261..4464)
                     /locus_tag="VGP50_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025905.1"
                     /translation="MRVEIARLNRLIARIKAKPGREWTVWSSTLHDLIEERELLGMVV
                     LNRGIEAGKKVVSFRRWRDGPWA"
     gene            complement(4548..5585)
                     /locus_tag="VGP50_00675"
     CDS             complement(4548..5585)
                     /locus_tag="VGP50_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEV8025906.1"
                     /translation="MTRAPFKAPLATGDIAPDCNLRSLDGETVELRGDAIAGHVVVLL
                     FCPKFAAASELLDAVRARLDALAGAGAKVFALTPEPAKIAAEQKLPFPMLHDREGEAF
                     HAFGVPARDAPSIILLRPNHHVLAVMKPAGPRELEEVVALAERLAAERRPVAMAPHPP
                     VLIVPDVLSPEDCRRLIKIYETRGNVFVEPGHGDDHMTGDYKMRIPEYGRGDRIDHWI
                     IDRDTNAFIDGRLQSRLFPEIRRAFQYRVTRRERMRIGCYTGARGGEAHGHRDDSEPI
                     SAHRRFATSINLNTEDFAGGALRFSEFGDQRYRPETGAAIVFSSSLLHEALQVTSGTR
                     LVLLAFLFGEH"
     gene            complement(5582..>5822)
                     /locus_tag="VGP50_00680"
     CDS             complement(5582..>5822)
                     /locus_tag="VGP50_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV8025907.1"
                     /translation="FEAYQVTCGITFTFGGLRIDGDAAVIDTEGEPIPGLYAAGELVG
                     GLFYFNYPGGTGLMSGAVFGRIAGTSAGAAATVAV"
BASE COUNT          889 a         2015 c         1936 g          982 t
ORIGIN      
        1 ttatgttttc ggccccaatt tcggcctgcc cgaccccagc cccttcgtcc tcaagaccac
       61 cgtgcagacc aagatgacgg gcctccccta ccgcaccgcc atcggccagc ttcccgaggc
      121 acccaagggc aagctgccct acatcgtcga cggcgatgtc acgctcggcg attcgacctt
      181 catccgggcg tatctcgagc acaagagcgg caccgatctc gaccacgggc tcgatgcgcg
      241 ggagcgcgcc ctgggctggg cggtcgagag gatgctcgag gaccatctct attgggccat
      301 ccttgagcat cgctggctcg atgctgacaa cttcgcgcgc ggtcccgcgc atttcttcga
      361 cggcgcgccc gaagcggtgc gcgacaagct gcgcgccgag gccgccgggc gcgtgcgcga
      421 gcggctccac ggccatggcc tcggccgcca cagccccgct gagatcgccg acctcggcgc
      481 gcgctcgctc gcttcgctcg gggccctcct gggcgagcgg ccctacctct ttggcgccga
      541 gcccaaagcc gcggacgcca ccgccttcgc cctggtcgcg gcggcggcgg tgccgctgtt
      601 tacgggaccg cttgccgacg cggcgcaatc cgatcggcgg ctcatggcct atcgcgatcg
      661 catgctgcaa cgcttctttg cggacttctc cgcggcggcg tgaagcgcgc gaggctccaa
      721 tcatccgtgc ccggttcgcc ctctttgcga ggcagggctt tcgtatatgg ggttcacacg
      781 gaggcacgga gaagaggggt tcggcgcgcg aagcgcgcca tccttcgctt ctccgtgttc
      841 tccgtgcctc cgtggtcaat ttttcctgtc tgcgtctcgg cagcatagcg atcgccgtgc
      901 tctgcgaggg gtgatccatc ctgggtcgga acactgagcg ctgacaggcg gctcgaccct
      961 tccttcggcg tcttgtatcc tcgcgcgcca tcagtacggg aatgggtgct tgcgacgcga
     1021 gcaagacatt ggaggaatgg tcgggtcatc gtctatttct cgtcgcgatg tcctcgccgg
     1081 gagcttggcg cttgccgcga ccgtcgcgct tccgcatcgc gcatcggcgg tgccgcccgc
     1141 gcgctatcgc cgctggaacg tctccgatcc cgattgcccc tcgcgcgtgc ttcaaagtta
     1201 caagaaggcg atccgcgcca tgctggcgct gccgcccggc gatccgcgca actggtaccg
     1261 ctacacgctc attcacgcgc tcgattgcgc ccatggcaat tggtggttcc tgccctggca
     1321 tcgcgcctat atcggctggt tcgagcggat ctgccgcgag ctctcgggcg atcccgaatt
     1381 cgcgctgccc tattgggact ggacccggga gccgcgcgtg ccgaaggcga tgttcgagga
     1441 cgtgctcgat ccgaacgacc gcgccttcat cgccacttcc ggcgcgttca aggcggcgta
     1501 ccaggacgcc gtcgccgctg ccggctattg gcgcatgacc cggaacgccg agctcgatct
     1561 tcgcccgtcg ccgcaatatg ccggattgct cgagcgctcg atccgctcgc ccgaggatct
     1621 ctggttcaac atcattgcgg acccggccgg tccgatgttc ttcgaccagc cccatgcgcg
     1681 cgggctctcg gcatccgcgc cggagctgac cgttccggac gcgccgccgg agcggcagcc
     1741 cgtgctcgtc gccgtcgcgg cggcgacgct cggcgatgcg ctggcgcccc gcgatttcac
     1801 ttccttcgcc ggtccgaaag tgcccgcgca cagcgtcctg tcgggattcg gcgtcttgga
     1861 atcgcagccg cacagcctcg tccatgaatg cctcggcggc ggctacaacg gcgcgggcgg
     1921 cttcatgagg ggcctgatgt cgccggtcga tccgatcttc tatcttcacc acaccaatat
     1981 cgaccgtctc tgggacatat gggcgcggaa gcaacgggcg gcgggcgatc cggcccttcc
     2041 cgagggctat tcggactcga ccgggcggaa ggcggcgggc gattccgact ttgcggcctg
     2101 ggcgcgcgag ccgtttctct tctttgtcga caccaaagga gatccggtcc gccagagcac
     2161 cgccggcgcc tatgccgaga tcggcgattt cgactatgac tacgcgccgg ggtcgggcga
     2221 ggaggtcgtg cccgctaccg catcgcccgc cgagacggcg gcagcgccgc gaaagactct
     2281 gcccgtccgc ctcgtcgccg gccggatcgc cgagagcagc ccggcgcgcg gatcggtcgt
     2341 ggtgccctgg gcgctcgtcg cgcagcaggc cgagccgcag caaccgcgac tcttcgcgtc
     2401 cgtcaccatc agcgtgccgc cgcatcgcca taccgccttc gacgtcctcc tcgccggcgc
     2461 ggccggcgcg ccgccgatct tcgtcgcctc gctcttgacc ttcggccatc acgggatggc
     2521 gggcccggtt tcattccgcg tgccgttgtc cgccgcgctt gccaaggcgc aggccaacca
     2581 cacatcgggg gacgatctca cgctcgacat cctggtcgcg cagcgcgccg gcagccgcgg
     2641 catggcggcg atggccggcc cgtcgccggg cgccagcgag gtgaccgcga tgtccctcga
     2701 tcagctctga cgcgcaacgg aggcatccca tgatgaggcg gttgtcggcg atcgctcttt
     2761 tgtccgcgct cgtttccgcg gccgcgctgg cgcagacccg tttgccgtcg gacgcagcga
     2821 tcctggccgg caaatggacc tatcgcagct acgtcaacag ccccgctctc gtcgagcgcg
     2881 acaaggacaa ggcactggcg atcatcttcg gcgaaggcac ttacgccttc gatccgccct
     2941 cgggcgcggc gatcaccggc acgctcgacc tgggcggcgg ccgtgttctc gatctcaagg
     3001 gcagcatcac gcagaccgcg ccgctgtcgc tggcgatcgc cggctacggg cgcgccggga
     3061 cgccgaccga gggctgggaa tacgactaca acgcctccct cgcctatgaa tggcccgctg
     3121 gcgtcgatca gagaccggcc ttggtcggaa gcgtgatccg cgccaagccc catggcagcg
     3181 gccccgccgg cgtcgtcgcc tccttcatcg ccgtgaagca gccctgaagg aaactacagc
     3241 aagccggcgc gctgctccgt ttgcacgaga tagtggcgcg cggcgaagcg gtagaactca
     3301 tcgagcgccg cttccgtctc ggtgccgcgg accggatcga gggccgtcca gatggcgatc
     3361 acgtcgtccc acggcatgag ccgcatatga acgtcgtcgc gcgcgcggcg aatctgctgc
     3421 acgtgatcgt cgatgccgag cagcaccgtc agaatctccg acgtcgccgc gtcgaggcgg
     3481 tcgaaggcgt gctcgaattc gccgtagggc cgctgcagca gcgtgctcac gcgcgagatg
     3541 ttgtcgagga tggtgcggtc gccgcgataa agcttggcga gaacattgat cttggcggcg
     3601 accacgcgga gcagcgcaca gcgctcgcgc aaggcctcga tatagcagag ctcgcgcgcc
     3661 agccggtcga tgcgcgcaag aatttccgct ttccctgcat cgacgccgag cctctcggcg
     3721 atgccggcgg ccgcctgctc gagctgccgc gtcccattct cgctcgcgcc cagccaccgc
     3781 aacatgctga tctcggtatc ggccggcttc ccatctacgg ccgtcacggc gtgcgcgatg
     3841 aggcgccctc tagcggtggc gcggtgcccg tccgacacgc tccaggaggc cgtgccgtcg
     3901 aggatctcgc gcgggatgga atcgcccggg cggatctctt tgacgaagcg caggctcagc
     3961 tcgacgatcc tcaggagccg cccgtcggtc gacgcggcgt cgatcgcgaa ctctttgcgc
     4021 agccccggaa gccggagcgc cgcggcgagg ctcccgagcg cgatatggaa ggcgggctcg
     4081 gtgccgtccg ccgtgaaggc aaagcggccg ccgtcgactt tgaagacatt atgctcgaag
     4141 agaaaatgcg tactgcaatc gggcgcctgc ggcatgcggt tctcccggcg cacatctatt
     4201 gccatgccag tatatgtcag accggtaaac gagcgatgaa agcttgagtt taaccttgga
     4261 ctaggcccaa ggaccgtcgc gccagcggcg gaaacttact actttcttgc ccgcctcgat
     4321 cccacggttg aggacgacca tacccagaag ttcacgttcc tcgatgagat cgtggagcgt
     4381 cgaggaccac accgtccatt cgcgccccgg cttcgctttg atgcgggcga tgagccggtt
     4441 gaggcgggcg atctcgaccc tcaagtctcg ggtttttcga ctcatgatcg gcagagttaa
     4501 cgccgcgttg ccgagttttg ctcccgcgcc ggccgcagcc ggcgcgatca atgctcgccg
     4561 aagaggaagg cgagaagcac gagcctggtc ccgctcgtca cctgaagcgc ctcgtgcagc
     4621 agcgagctgg agaagacgat ggcggcgccg gtctccggcc ggtagcgctg gtcgccgaac
     4681 tcggaaaaac gcagcgcgcc gccggcgaaa tcctcggtgt tgagattgat cgaggtggcg
     4741 aaacggcgat gcgccgagat cggctcgctg tcgtcgcggt ggccatgggc ctcgccgccg
     4801 cgcgcgccgg tatagcagcc gatgcgcatc cgctcgcgcc gcgtgacgcg gtattggaag
     4861 gcgcggcgga tctccggaaa gaggcgggac tgcagcctgc cgtcgatgaa ggcgttggtg
     4921 tcgcgatcga tgatccagtg atcgatgcgg tcgccgcggc catattcggg aatgcgcatc
     4981 ttgtagtcgc cggtcatgtg gtcgtcgcca tggcccggct cgacgaagac gttgccgcgc
     5041 gtctcgtaga tcttgatgag gcgccggcaa tcctcggggc tcagcacgtc cggcacgatg
     5101 agcacgggcg ggtgcggcgc catcgccacc ggccgccgct cggcggcaag acgctcggcc
     5161 agcgccacga cctcctccag ctcccggggc ccggccggct tcatgacggc gagcacatgg
     5221 tgattgggcc gcagcaggat gatgctgggc gcatcgcgtg ccggcacgcc gaaggcgtga
     5281 aaggcctcgc cttcgcggtc gtgcagcatg ggaaacggga gcttctgctc ggcggcgatc
     5341 ttggcgggct cgggcgtcag cgcgaaaact ttggcgcctg ccccggccaa cgcgtcgagg
     5401 cgcgcgcgga cggcatcgag cagctcgctc gcggccgcga atttcggaca gaacagcagg
     5461 accacgacat gcccggcgat cgcatcgccg cggagctcga cggtctcgcc atcgagcgac
     5521 cgcaggttgc agtcgggcgc gatgtcaccc gtcgcgaggg gcgctttgaa cggcgcgcgc
     5581 gtcacaccgc gacggtcgcc gccgcaccgg cgctggtgcc ggcgatgcgg ccgaagacgg
     5641 cgcccgacat gaggccggtg ccgcccgggt agttaaaata gaagaggccg cccaccagct
     5701 cgcccgcggc atagaggccg gggatcggct cgccctcggt gtcgatcacc gcggcatcgc
     5761 cgtcgatgcg caagccgccg aaggtgaagg tgatgccgca ggtcacctga taggcctcga
     5821 ag
//
LOCUS       DASYNS010000019         4639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2500439, whole genome shotgun sequence.
ACCESSION   DASYNS010000019 DASYNS010000000
VERSION     DASYNS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2500439"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="VGP50_00685"
     CDS             complement(<1..1000)
                     /locus_tag="VGP50_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEV8025908.1"
                     /translation="MGKSTGFLEFQRRERGYAPVPERVQHWREFVRPLPEDELRRQGG
                     RCMDCGTPYCHDACPVNNIIPDWNDLVFENDMKTALAVLHQTNNFPDVTGRICPAPCE
                     AACTLNLVEQPVTIKTIEHSISERGWSEGWIEPQIAMRRSGKRVAVIGSGPAGLACAQ
                     QLARAGHEVAVFEKAARIGGLLRYGIPDFKLEKHVIDRRLAQMRAEGVEFHPNCHVGA
                     NVSVERLTREHDAIVIAIGAEAPRDLPVPGRELKGIHFAMEFLTQQNQRVAGEKVPEA
                     GSILATGRDVLVIGGGDTGSDCIGTSNRHHARSVSQLEILPLPPQHEDKLMTWPNWP"
     gene            complement(1004..>4639)
                     /gene="gltB"
                     /locus_tag="VGP50_00690"
     CDS             complement(1004..>4639)
                     /gene="gltB"
                     /locus_tag="VGP50_00690"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEV8025909.1"
                     /translation="RRAFYEYHAALMEPWDGPAALAFTDGRQIGATLDRNGLRPARFL
                     VTSDDLVVMASEAGVLDIPEKHIIRKWRLEPGKMLLVDLERGRIIDDAELKAEISTAK
                     PYQAWLDKTQIKLETLPDEIAPMPPPAHVLLDRQQAFGYTQEDIKFFLRPMALAADDT
                     IGSMGRDTPLAVLSLRPKLLFDYFQQRFAQVTNPPIDSIREELVMALVSLVGPRPNLL
                     DLKTGGRHIRLELKQPILTNVDLEKIRRIEDNTSGKFKTYTLAICYPASEGAAGMAHA
                     VESMCRRAEEVVRTGHNLLVLSDRALDAEHIAVPSLLAVAAVHHHLIRGGLRTEVGLV
                     IETGEARQLHDMCLLAGYGAEAINPYLAFDTIATQRREAGLNGTEEAHKRYIKALAKG
                     IMKVMSKMGICTFQSYCGAQIFDAVGLSSAFLDKYFTGTVSPVEGAGLEEIAEETVRR
                     HRLAYGDAPNLRDALDPGGEILYRVNGEAHIWTADTVALLQHAVRGNDYRKFKDYARR
                     VGEQDGAAIALRALLDFKFAENPVPLEEVEPAAEIVKRFSGGAMSFGSISWEAHTTLA
                     IALNRLGGRSNTGEGGEDSARYKPLPNGDSMRSAIKQVASGRFGVNIEYLVNASDLQI
                     KMAQGAKPGEGGQLPGHKVDDWIARVRRSTPGVGLISPPPHHDIYSIEDLAQLIHDLK
                     NANPEARISVKLVSEIGVGTIAAGVAKAYADHVTIAGHEGGTGASPLTSIHHAGEPWE
                     IGLAETHQTLVLNGLRGRIAVQVDGGLRSGRDVVVGALLGADEFGFATAALVAAGCVM
                     MRKCHLNTCPVGVATQNPELRHRFIGKPEDVTNFFLFIAEDVREHMAKLGFRTVNEMI
                     GRTDKLEMRRGLNHPKARLVDLSRVLYRPAVKAGTATHQCEKQNHRLQDALDHKLIAK
                     AKHALARKKPVTIEMPIRNVHRVVGAMLSGEVAKRYGDEGLPDGSIHVRFKGTAGQSF
                     GAFLARGITLELEGATNDYCGKGLSGGRLVVYPPHDSLLERDKSIIVGNTVLYGAMAG
                     ECFFSGVAGERFAVRNSGAIAVVEGVGAHGCEYMTGGTVLVLGETGSNFAAGMSGGIA
                     YVLDERGDFERRCNLAMVELEPIADEDSALETLEHRGGDIEAHGLVDVAHSLTQNDEQ
                     LLRRLIARHVRYTDSPRAKLILSGWDSYRAKFVKVMPIEYRRALQQIQARSRVAERPE
                     VSVAVGD"
BASE COUNT          731 a         1519 c         1549 g          840 t
ORIGIN      
        1 agggccagtt cggccaggtc atgagcttgt cctcgtgctg cggcggcagc ggcaggatct
       61 cgagctggct gaccgatcgc gcatgatgcc ggttcgatgt gccgatgcag tcggagcccg
      121 tgtcgccgcc gccgatcacg aggacgtcgc ggcccgtcgc gagaatgctt ccggcctctg
      181 gcaccttctc ccccgcgaca cgctggttct gctgggtgag gaactccatg gcgaagtgga
      241 tgcctttgag ctcgcggccg ggtaccggca ggtcgcgcgg cgcctcggcg ccgatggcga
      301 tgacgatcgc atcgtgctcg cgcgtcaggc gctcgaccga gacgttggcg ccgacgtggc
      361 agttcgggtg gaactccacg ccttcggcgc gcatctgcgc gagccgccgg tcgatcacat
      421 gcttctcgag cttgaaatcg ggaatgccgt agcgcagcaa tccgccgatc cgcgctgcct
      481 tctcgaacac cgccacctcg tgcccggcgc gcgccagctg ctgggcacag gcaagacccg
      541 ccggtcccga tccgatcacg gcgacccgct tgccgctgcg ccgcatggcg atctgcggct
      601 cgatccagcc ttcgctccag ccgcgttcgg agatcgagtg ctcgatggtc ttgatggtca
      661 cgggctgctc gacgaggttg agcgtacagg ccgcctcgca aggggccgga cagatccggc
      721 cggtgacgtc ggggaagttg ttggtctggt gaagcaccgc aagcgcggtc ttcatgtcgt
      781 tttcgaagac gaggtcgttc caatccggga tgatgttgtt gaccggacag gcgtcgtggc
      841 agtagggcgt accgcaatcc atgcagcgcc cgccttggcg gcgcagttca tcctccggca
      901 gcgggcggac gaactcgcgc caatgctgaa cgcgctcggg caccggcgca tagccacgct
      961 cgcgccgctg gaactccaga aagcccgtgg acttgcccat gtctcagtcg cctacggcca
     1021 cgctcacttc ggggcgttcg gcgacgcgcg agcgggcctg aatctgctgc agcgcgcggc
     1081 gatactcgat cggcattacc ttgacgaact tcgcgcgata gctgtcccag cccgagagga
     1141 tcagcttggc gcgcggactg tcggtatagc gcacatgccg cgcgatgagg cggcgcaaca
     1201 gctgctcgtc attctgggtg aggctgtgcg ccacgtccac cagcccatgg gcctcgatgt
     1261 cgccgccccg gtgctcgagc gtttcgaggg ccgaatcctc gtccgcgatc ggctcgagct
     1321 cgaccatggc cagattgcag cgccgctcga aatcgccgcg ctcgtccagg acatacgcga
     1381 tgccgccgct catgccggcc gcgaagttgc tgccggtctc gccgagcacc agcacggtgc
     1441 cgcccgtcat gtattcgcag ccatgggcgc cgacgccctc gaccacggcg atcgcgcctg
     1501 aattgcgcac ggcgaagcgc tcgccggcga cgccgctgaa gaagcattcg ccggccatgg
     1561 cgccatagag aacggtgttg ccgacgatga tgctcttgtc ccgctcgagc aggctgtcat
     1621 ggggcgggta gaccacgagg cggccgcccg acaggccctt gccgcaatag tcgttggtgg
     1681 caccttcgag ctcaagcgtg atcccgcgcg cgaggaaggc cccgaagctc tggcccgcgg
     1741 tgcccttgaa gcgcacatgg atcgacccat ctggcagacc ctcgtcgcca tagcgtttcg
     1801 ccacttcgcc tgacagcatg gcgccgacga cacgatggac gtttcggatc ggcatctcga
     1861 tggtcaccgg ctttttccgc gccagcgcgt gcttcgcctt ggcgatgagc ttgtggtcga
     1921 gagcgtcctg aagccgatga ttctgcttct cgcactggtg ggtcgcggtt cccgctttga
     1981 cggccggacg gtagaggacg cgcgagaggt cgaccagccg cgcttttggg tgattgaggc
     2041 cccgccgcat ctcgagcttg tcggtgcgcc cgatcatctc gttgacggtg cggaagccga
     2101 gcttcgccat gtgctcgcgt acgtcctcgg cgatgaacag gaagaaattc gtcacgtctt
     2161 cgggcttgcc gatgaaacga tggcgcagtt ccgggttctg cgtcgccacg ccgacggggc
     2221 aggtgttgag atggcatttg cgcatcatca cgcagcccgc ggcaacgagc gctgccgtgg
     2281 cgaagccgaa ctcgtcggca ccgagcagcg ctcccacgac gacatcgcgg ccagagcgta
     2341 atccgccgtc gacctggacg gcgatgcgtc cccggagccc gttcagcacc agcgtctgat
     2401 gtgtctcggc gagcccgatc tcccacggct ctccggcatg atggatcgag gtcagcgggc
     2461 tcgcgcccgt gccgccctca tggccggcaa tcgtgacatg gtcggcatag gccttggcga
     2521 cgccggcagc gatcgtgccg acgccgatct cggagacgag cttgacgctg attcgcgcct
     2581 cggggttcgc gtttttcagg tcatggatga gctgggccag atcctcgatc gaatagatgt
     2641 cgtgatgcgg cggcggcgag atcagcccga cccccggcgt cgagcggcgc acgcgggcga
     2701 tccagtcatc gaccttgtgg cccgggagct gcccgccttc gcccggcttc gcgccctgcg
     2761 ccatcttgat ctgcaggtcg ctggcgttga ccagatattc gatgttgacg ccgaaccgtc
     2821 ccgaggcgac ctgcttgatc gccgagcgca tggaatcgcc attgggcagc ggcttgtacc
     2881 gcgccgaatc ctcgccgcct tcgccggtgt tggatcttcc gccgagccgg ttcagcgcga
     2941 tggcgagcgt ggtgtgcgcc tcccaggaga tcgacccgaa ggacatggca ccgcccgaga
     3001 agcgcttgac gatttcggct gccggctcga cctcctcgag cggaacgggg ttctcggcga
     3061 atttgaagtc gagcagcgcg cggagcgcga ttgcggcccc gtcctgctcg ccgacgcggc
     3121 gcgcatagtc cttgaacttg cggtagtcgt tgccgcgcac ggcatgttgc agcagcgcta
     3181 ccgtgtccgc agtccagata tgcgcctcgc cattgacccg atagagaatc tcgccacccg
     3241 gatcgagcgc atctctgagg ttgggcgcgt cgccataggc aaggcggtgg cgccgcaccg
     3301 tttcctcggc gatctcctcg aggccggcgc cctcgaccgg tgaaaccgtg cccgtgaaat
     3361 acttgtcgag aaaagcgctc gacaagccga cggcatcgaa gatctgtgct ccgcaatagg
     3421 attggaaggt gcagatgccc attttggaca tcaccttcat gatccccttg gcgagcgcct
     3481 tgatatagcg tttatgcgcc tcctcggtcc cgttgaggcc ggcctcgcgg cgctgtgtcg
     3541 cgatggtgtc gaaggcaaga taaggattga tcgcctccgc gccgtagccg gcgagcaggc
     3601 acatgtcatg gagctggcgc gcctcgccgg tctcgatcac cagcccgacc tcggtgcgca
     3661 agccgccgcg gatcaggtga tggtgcacgg ccgctaccgc cagcaacgaa gggacggcga
     3721 tgtgttcggc gtcgagcgcg cgatctgaga gcacgagcag attgtgcccg gtgcgcacta
     3781 cttcctcggc gcgccggcac atgctctcga ccgcgtgggc catgcccgcc gcgccctcgc
     3841 tggcgggata gcagatggcg agcgtgtagg tcttgaactt gccgctggtg ttgtcctcga
     3901 tccgccggat cttctcgaga tcgacattgg tgaggatcgg ttgcttgagc tcgaggcgaa
     3961 tgtgccgccc gccggtcttg aggtccaaca ggttcggccg cggcccgacc agcgacacca
     4021 gggccatcac cagctcttca cggatcgaat cgatcggcgg gttggtgacc tgcgcgaagc
     4081 gctgctggaa atagtcgaag agcagcttcg ggcggagcga caacacggcg agcggcgtat
     4141 cgcgccccat cgagccgatg gtgtcgtcgg cggcgagcgc catcggccgt aggaagaact
     4201 tgatgtcttc ctgggtgtag ccgaaggcct gctggcgatc gaggagcaca tgcgcgggcg
     4261 gcggcatcgg cgcgatttcg tccggcagcg tctcgagctt gatctgcgtc ttgtcgagcc
     4321 acgcctgata cggcttggcg gtgctgatct cggccttgag ctctgcatcg tcgatgatgc
     4381 ggccgcgctc gagatcgacc agcagcatct tgccgggctc gagccgccac ttgcgaatga
     4441 tgtgcttctc cggaatgtcg agcacgcccg cttcggatgc catgacaacg aggtcgtcgg
     4501 acgtcacgag gaagcgcgcc ggacgcaggc cgttgcggtc gagcgtcgcg ccgatctgcc
     4561 ggccgtccgt gaaagccagc gccgccggcc cgtcccaggg ctccatcagc gccgcgtgat
     4621 actcgtagaa ggcgcgccg
//
LOCUS       DASYNS010000020         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1721327, whole genome shotgun sequence.
ACCESSION   DASYNS010000020 DASYNS010000000
VERSION     DASYNS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721327"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(562..>1776)
                     /locus_tag="VGP50_00695"
     CDS             complement(562..>1776)
                     /locus_tag="VGP50_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV8025910.1"
                     /translation="DRIDLMKKVEDRLAANLATTAQRVSGAAVTEFNVVLAIVLGLLI
                     VSGPLAMLMIRSIVGPIRAMTGAMTMLAGGDLEIAVPALGRRDEIGAMAAAVGVFKSN
                     AVETRRLTADQRREQARKEERTTKIEAYITAFQRRVAEALEALTSASTEMRAAAESMT
                     GIAQETSRQLHAVVSASEQASANVQTVAAATEEMATSISEIGRQAGQSSEIAVTAVAD
                     AERTTNTMRELAVAAQKIGAVISLIHHIARQTNLLALNATIEAARAGDAGKGFAVVAS
                     EVKLLANQTGSATDEIRDQIGGMQSVTQEAVAAIERVYETINRMNQIVATIAAAVGQQ
                     TVTTREITRNTQEAARGTEDVSLTMTNVNHAANETGAAAAQVRNSADALARQAEALRS
                     DVGGFLDNIRAA"
BASE COUNT          251 a          594 c          606 g          325 t
ORIGIN      
        1 tgtgccctcg ccatagtaag gtcgactggg ccctaggatg ttttgtccgc tttcgggaag
       61 tcgatcgcgg tacacgggtg accgacctgg gcgcatctcc gacgaacggc tcgacgcagc
      121 aagtctgaaa tcggaccggc agcagtcgca ggcgtgctcc tttaccctcg aggagtatgc
      181 gcgcgagacc cagtcgcgct ttgccgagca gctgctggcc gactgggacc gcgaggtccg
      241 ccgcttctga cagatcgtgc cgaaggaaat gctgacgcgg ctgccgcaac cgctcaaggc
      301 cgagcagcgg aaggtggaag cgggcgatta gtctaggcca gcgcagagca tgaacattat
      361 cctctccgcc cgctcttgcg gggggagagg gcgcgagcgc agcgagcggg tgaggtgggt
      421 gtcgatgccg gattcgctgg tgctgtacgc gacgacgctt cgcgtcgccc gggacacctc
      481 accctcccgg cgcgccgcgc cgggcccctc cctctccccg cttcgcgcgg agagggtatg
      541 atccagctcg gatcaagcgc gtcaggcggc gcggatgttg tcgaggaaac cgccgacatc
      601 gctgcgcaag gcttcggcct gacgggcgag cgcatcggcc gagttccgca cttgcgccgc
      661 cgcggcgccg gtctcgttgg cggcgtggtt gacgttggtc atcgtcaggg acacgtcctc
      721 ggtgccgcgc gccgcctcct gagtgttgcg ggtgatctcg cgcgtcgtca ccgtctgctg
      781 ccccaccgcc gcggcgatgg tggcgacgat ctggttcatg cgattgatcg tctcgtagac
      841 gcgctcgatg gcggcgaccg cctcctgcgt caccgattgc atgccgccga tctggtcgcg
      901 gatctcgtcc gtcgcgctgc cggtctgatt cgccagcagc ttcacctccg aggcaaccac
      961 ggcaaagcct ttgccggcgt cgccggcgcg cgccgcttcg atcgtcgcat tgagcgcgag
     1021 cagattggtc tggcgggcga tgtgatggat caggctgatc acggcgccga tcttctgcgc
     1081 cgccacggcg agctctcgca tggtgttggt ggtgcgctcg gcgtcggcca cggcagtgac
     1141 ggcgatctcg gaggattgtc cggcctggcg gccgatctcg ctgatcgagg tcgccatttc
     1201 ctcggtcgcc gccgccacgg tctgcacatt cgccgaggcc tgctccgacg cggagaccac
     1261 ggcgtgcagc tggcggctgg tctcctgggc gatgccggtc atgctctcgg cggcggcgcg
     1321 catctctgtc gatgccgatg tcagcgcctc gagcgcctcc gcgacccggc gctggaaggc
     1381 ggtgatatac gcctcgatct ttgtcgttcg ttcctctttc cgagcctgct cccggcgttg
     1441 gtcagcagtc aatcgtcgcg tttcgacggc gttgctcttg aagacgccga ccgccgcggc
     1501 catggcgccg atctcgtccc gccggcccag cgccggaacg gcgatctcga gatcgccgcc
     1561 agccagcatg gtcatcgccc ccgtcatcgc ccggatcggg ccgacgatgc tgcggatcat
     1621 cagcatcgcc aaggggccgg aaacgatgag caagccgaga acgatggcga gcacgacgtt
     1681 gaattcggtc accgcggcgc cgctgacgcg ctgcgccgtg gtggcgagat tggcggccag
     1741 gcgatcctcg accttcttca tcaggtcgat ccgatc
//
LOCUS       DASYNS010000021         5728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_629205, whole genome shotgun sequence.
ACCESSION   DASYNS010000021 DASYNS010000000
VERSION     DASYNS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629205"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VGP50_00700"
     CDS             <1..1221
                     /locus_tag="VGP50_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR05002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat
                     NxxGxxAF-containing protein"
                     /protein_id="HEV8025911.1"
                     /translation="RAPQSETAAPAAPAALERVALSGQASPAGGSFDRFDLGSEAIPA
                     PANSRGEVSFFATLLRGQGEEGLFLAGKDGVAKIAAVGDKVPSGESIAGFGEHPAPTL
                     NEAGSVAFAATLTGGKAQSGVFRVDKGRITALALSGAAAPDLPGGTLAQFEPPALNDK
                     GHVAFLAELRRGRELEQAIYIHRGDRLKKLIAAGDPAPGGGVFSSFGNPAINNRGDVA
                     FAAIMEQGPNLGGVFLSSDNELRLVLAVGAPAPTGGIFARFSERLALNDAGTIALSAV
                     LRQAGPSAAIFTIENDTARAVATVGDGVPGGGTIAALASWPVLSQSGAVAFIASVDGG
                     PNGLGVYLAGAGEPKRVIATGDALPDGSRLAGFPLYPAIAIGGKDTVTFAATAERDGV
                     RTDALFDYGPPRRR"
     gene            1302..1676
                     /locus_tag="VGP50_00705"
     CDS             1302..1676
                     /locus_tag="VGP50_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV8025912.1"
                     /translation="MPELDPTAVARRSYQAYVNKDRAALELLIADDFHFTSPLDNRID
                     RATYFRRCWPNSAGITGFDFVHVVADGNRVYVTYEGRNTGGKRFRNTEILTIRDGQIF
                     DAEVYFGWSIPHEAPAGGSIDH"
     gene            complement(1742..2308)
                     /locus_tag="VGP50_00710"
     CDS             complement(1742..2308)
                     /locus_tag="VGP50_00710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEV8025913.1"
                     /translation="MSAAPPPPGSVLLLIDLQQAIDHPSWGERNNPSAEAQIMRLLRH
                     WREAGWPVWHVRHDSMDPRSHYRPGQPGNNFKVEAAPIAGETVIRKRTNSAFIGTDLE
                     ERLRAGGHNCLVVVGVITNNSVEATVRMAGNLGFSTHLVADGCFTFERTDWNGTFRSA
                     EDVHAMSLANLDGEYCTVVATATLLGKA"
     gene            complement(2369..3400)
                     /locus_tag="VGP50_00715"
     CDS             complement(2369..3400)
                     /locus_tag="VGP50_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV8025914.1"
                     /translation="MPLRGGDPAPVIGRRSFLGLAAALLAVPAAQSEEARRYKVGFAN
                     LTEDPGVRLEGFGFTGADVQTSFTLAARTLPIDMVFFDNDRDRAKTLANAESAVAQRL
                     DAFVEYSDDASANAELAKRLKAAGIPIVAITYPVPGAPLYGPDNQLAGRIAGDALAGF
                     ALSHWAGMPQVAVLLGDLANSADHMTERVEGIAAALKEKLPDAPLTRLDSGGNGTQAE
                     ALLRRFAAAQTGKKLLIAALDDITALRAKSAVEEAGRANDSAIVSQGCDHTVHGGMSD
                     RKELDPSNRGSILIGSVAYRLDRYGYDVLPLVLKLLSGEPVPPRVATRHVLVSAANVF
                     SVYPPIDMN"
     gene            complement(3409..4425)
                     /locus_tag="VGP50_00720"
     CDS             complement(3409..4425)
                     /locus_tag="VGP50_00720"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEV8025915.1"
                     /translation="MRTTLGLAICALAGVLTVSSGAFAQSDCSKTVPASPFGKDDQTG
                     ATNRVTPAVTKAAAAEIQTGKVTTLTNPLVDGVPLFGTRFTKTVLTAVTLAPGAAIGD
                     NNLTFMEDTWLSQSHVGTHLDGMGHIGRGDCYYNQTAMGKFINQNNMTKLGLEHLKSF
                     ATRGVLIDLVRVYQEANKLKTNAACRKPCIDKGTVITPEDLQAGLKMYNVTLREGDAV
                     FLNTGWGDVFAQYPAQNALYNSGEPGLGKAAAKWLIDQKVIAVGADTWAVEVIPGEVQ
                     GEAFAVHQMLITDNGIHIIENVRTDLLADEVASGKRATFFLNMSVPKAVGLTGNFVGI
                     EAIQ"
     gene            4618..4974
                     /locus_tag="VGP50_00725"
     CDS             4618..4974
                     /locus_tag="VGP50_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV8025916.1"
                     /translation="MSPDHLSTTFAALADPTRRAILARLAAGEASVTELGAPFNMSLP
                     AISKHLKVLERARLIERGREAQWRPCRLAAAPLRDVDDWLRHYRRFWEENFDRLGDYL
                     KDLQAKEKRHGRKKRK"
     gene            4952..5491
                     /locus_tag="VGP50_00730"
     CDS             4952..5491
                     /locus_tag="VGP50_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV8025917.1"
                     /translation="MGGKNENSAPPAGDPALVITRVFHAPRSLVWKAWTEPERLARWW
                     GPKGFTMLKAMLDLRPGGLFHYGMRSPDGQEMWGKFVYREIAAPERMVFVNSFSDAKG
                     GTTRAPFAATWPLEVLNTLLIAESGGRTTLALRGAPLNATDQERATFAAAQKSMQQGF
                     AGTFEQLDAYLATQRSNRQ"
     gene            5488..>5728
                     /locus_tag="VGP50_00735"
     CDS             5488..>5728
                     /locus_tag="VGP50_00735"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV8025918.1"
                     /translation="MTATANTRRENPKVERSSRHGMFVVERRYDARPALVFAAWASPE
                     AKSRWFHGPRGEWTENLRELDFRVGGRERLLGTRMS"
BASE COUNT          943 a         1945 c         1864 g          976 t
ORIGIN      
        1 cgcgcgccgc agagcgaaac cgcagccccc gccgctcccg ccgcgctcga gcgcgtcgcc
       61 ctgagcggac aagcctcgcc cgccggcggc agcttcgacc gcttcgatct cggtagcgag
      121 gcgattcccg cgcccgccaa cagccgcggc gaagtgagct tcttcgcgac gctgctgcgc
      181 ggtcaaggcg aggagggcct gttcctcgcc ggcaaggacg gcgtcgccaa gatcgccgcc
      241 gtcggcgata aagtccccag cggcgagagc atcgccggct tcggcgagca tcccgcgccg
      301 acgctgaacg aggcggggag cgtcgctttc gccgcgacgc tgaccggcgg caaggcgcaa
      361 agcggcgtct tccgcgtcga caaaggccgc atcaccgcgc tcgctttgag cggcgctgcc
      421 gcgcccgatc tccccggcgg cacgctcgcg caattcgagc cgccagcgct caacgacaag
      481 ggccacgtcg ctttcctcgc cgagctccgt cgcgggcgcg agctcgaaca ggcgatctac
      541 atccatcgcg gcgaccggtt gaagaaactc atcgccgccg gcgacccggc gccgggcggc
      601 ggcgtctttt cgagcttcgg caatcccgcg atcaacaacc gcggcgacgt cgccttcgcc
      661 gcgatcatgg agcaggggcc caatctcggc ggcgtcttcc tcagcagcga caacgagctc
      721 cgcctcgtgc tcgcggtggg cgcgccggcg cccacgggcg gcatcttcgc gcgcttctcg
      781 gagcgcctcg cgctgaacga cgccggcacc atcgcgctca gcgccgtgct gcgccaggcc
      841 ggcccgagcg cggcgatctt cacgatcgag aacgacacgg cgcgcgccgt ggcgaccgtg
      901 ggggacggcg tgccgggcgg cggcaccatc gccgcgctcg cctcctggcc cgtgctgagc
      961 cagtccggcg ccgtcgcctt catcgcctcg gtcgatggcg ggcccaacgg gctcggcgtc
     1021 tatctcgccg gcgccggcga gccgaagcgc gtgatcgcca ccggcgacgc cttgcccgac
     1081 ggcagccgcc tcgccggttt ccccctctat cccgcgatcg ccatcggcgg caaggacact
     1141 gtcactttcg ccgcgaccgc cgagcgcgac ggcgtgcgca cggacgcgct cttcgattac
     1201 gggccgccgc ggcggcggta ataaagcagc aagggcctcg gcgccggcgc cggaaccatg
     1261 caggggtggc gaggttgatc cggcaaggag gggctgccgc catgccagaa ctcgacccga
     1321 ctgctgtcgc tcgcaggagc tatcaggctt acgtcaacaa ggaccgcgcc gcgctggaac
     1381 tgctcatcgc cgacgatttc cacttcacca gcccgctcga caatcgcatc gatcgtgcca
     1441 cctatttccg acgctgctgg cccaacagcg cagggatcac cggcttcgat ttcgtccacg
     1501 tggtggccga cggcaatcgc gtctatgtta cctatgaagg acggaatacc ggcggcaagc
     1561 gcttccggaa cacggagatc ctcaccatcc gcgacgggca gatcttcgac gccgaggtct
     1621 atttcggctg gtcgatcccc cacgaagcgc cggccggcgg ctcgatcgat cactagcctg
     1681 gatcgacgac ttattactca aaagcagtcg cggaatgtct cgacgagcgg agagagcgaa
     1741 tctacgcttt cccaagcaat gtcgccgtcg cgacgacagt gcaatactcc ccgtccaagt
     1801 tcgcgagcga catcgcatga acatcttccg cgctacgaaa cgtgccgttc caatccgtgc
     1861 gctcgaaggt gaagcagcca tcggcgacga gatgggtcga aaatccgaga ttgcccgcca
     1921 tgcgaactgt cgcctcgacc gagttgttgg tgatgacgcc caccacaacc aagcagttgt
     1981 ggccgccggc ccgaaggcgc tcctctaaat ccgtgccgat gaacgcgctg ttggtgcgtt
     2041 tgcgaatcac tgtttcgccc gcaatcggtg ccgcttcgac tttgaaatta ttgccgggct
     2101 ggccggggcg gtagtgtgag cgcgggtcca tcgagtcgtg gcggacatgc cagacaggcc
     2161 atcctgcctc acgccagtgt cgcagcaggc gcatgatctg cgcttcggcc gacgggttat
     2221 tgcgctctcc ccagctcgga tgatcgattg cctgctgaag gtcgatgagc agaagaacgg
     2281 aacccggtgg aggaggtgcg gcgctcacgg attgagtttc ccgcgtaacc cacgcgacag
     2341 caagagggtc gaatctccgc gtgtatgttt agttcatatc gatcggcgga tagaccgaga
     2401 atacgttcgc ggccgacacc agcacatgcc gtgtcgccac gcgcggcggc accggctcgc
     2461 cgctcagcag tttcagcacc agcggcagca cgtcgtagcc gtagcggtcg agccggtagg
     2521 cgaccgagcc gatgaggatg ctgccgcggt tgctcggatc gagctccttc ctgtcgctca
     2581 tgccgccatg gaccgtatga tcgcagccct ggctcacgat cgccgaatcg ttggcgcggc
     2641 cggcctcctc gaccgccgac ttggcgcgca acgcggtgat atcgtcgagc gcagcgatca
     2701 gcagtttctt gcctgtctgc gctgcggcga agcggcgcag cagtgcttcg gcctgggtcc
     2761 cgttgccgcc gctgtcgagc cgcgtcagcg gcgcatcggg gagcttctct ttgagcgcgg
     2821 cggcaatgcc ctcgacgcgc tccgtcatgt gatcggcact gttggcgagg tcgcccagaa
     2881 ggacggcgac ctgcggcatg cccgcccaat ggctgagcgc gaagcccgcc agggcgtcgc
     2941 ccgcgatgcg gcccgcgagc tgattatcgg ggccgtagag cggcgcgccg ggcaccggat
     3001 aggtgatggc gacgatcggg atgccggccg ctttcaggcg cttcgccaac tcagcattgg
     3061 ccgaggcgtc gtccgaatat tcgacgaaag catcgagccg ctgcgccacc gcgctctccg
     3121 cattggccag cgtcttcgcg cggtcgcggt cgttgtcgaa gaacaccatg tcgatcggca
     3181 gcgtccgggc ggcgagcgtg aagctcgtct gcacatcggc gccggtgaat ccgaagccct
     3241 cgagccggac gcccggatcc tcggtcaggt tcgcgaagcc cactttgtag cgccgggcct
     3301 cttcgctctg cgctgccggt accgccagca gcgccgctgc cagcccgaga aacgaccggc
     3361 gcccgatgac gggcgccgga tcacctccac gaagcggcac ggaaggcgct attggatcgc
     3421 ctcgatgccg acgaagttgc cggtaaggcc caccgccttg ggcaccgaca tgttgaggaa
     3481 aaaggtcgcc cgcttgcccg acgcgacctc gtcggcgaga agatcggtgc ggacattctc
     3541 gatgatgtgg atgccgttat cggtgatcag catctgatgc accgcgaaag cttcgccctg
     3601 gacctcgccg ggaatcactt cgaccgccca ggtatcggcg ccgaccgcga tgaccttctg
     3661 gtcgatgagc catttggccg ccgctttgcc gaggccgggc tcgccgctgt tgtagagcgc
     3721 gttctgcgcc ggatattgtg cgaacacgtc gccccagccg gtgttcagga acaccgcgtc
     3781 gccttcgcgc aaggtgacat tgtacatctt gagaccggcc tgcagatcct cgggcgtgat
     3841 gactgtgccc ttgtcgatac aaggcttgcg gcaggcggcg ttggtcttga gcttgttcgc
     3901 ttcctgatag acgcgcacca ggtcgatgag cacgccgcgc gtcgcaaagg atttgagatg
     3961 ctcgagcccg agcttcgtca tgttgttctg gttgatgaac ttgcccatcg cggtctggtt
     4021 gtaatagcag tcgccgcggc cgatatggcc catgccgtcg agatgcgtcc cgacatggct
     4081 ctggctcagc caggtgtctt ccatgaaggt caggttgttg tcgccgatcg cggcgccggg
     4141 cgcgagcgtc actgccgtca ggacggtctt ggtgaagcgc gtgccgaaga ggggcacgcc
     4201 gtccaccagc ggattggtga gggtcgtgac cttgccggtc tggatctcgg ccgccgcggc
     4261 cttggtcacc gccggcgtca cgcggttggt ggcgccggtc tggtcatcct tgccgaaagg
     4321 cgacgccggc acggttttgc tgcaatcact ttgtgcgaaa gcgccggatg agacagtcaa
     4381 tacgcctgcc agcgcgcaga tcgcgagtcc gagcgtcgtc ctcatcgtcc cctcccctta
     4441 tcccctggat cagtcttcgt attggttaaa gtgtcgcgcc atccggccgc ggcgtcaacc
     4501 gctcgctccg tgccgggtgt ccccgtcatt tcacggcgtg cttgacacgg cgccgccgtc
     4561 tgtcgtattt aactgatcag ttcagtaact agtcggttaa gtaaggaccg aggacggatg
     4621 tcgcccgacc acttgagcac cacctttgcg gccctcgccg atccgacgcg gcgcgcgatc
     4681 ctcgcccgcc tcgccgcggg agaagcctcg gtcacggagc tgggtgcgcc cttcaatatg
     4741 agccttccgg cgatttcgaa gcatttgaaa gtgctggagc gcgcccgcct catcgagcgc
     4801 ggccgcgagg cgcagtggcg gccgtgccgg cttgcggcgg cgccgctccg cgatgtcgac
     4861 gactggcttc ggcactaccg ccggttctgg gaggaaaatt tcgaccggct gggcgattac
     4921 ttgaaagacc tacaagccaa ggagaagagg catgggcgga aaaaacgaaa atagcgcgcc
     4981 gccggcgggc gacccggcgc tggtcatcac gcgggtcttc cacgcgccgc gcagcctggt
     5041 gtggaaagcc tggaccgagc ccgagcgcct ggcgcgctgg tggggcccca agggcttcac
     5101 catgctgaag gcgatgctcg atcttcgccc cggcggcctc ttccactatg gcatgcgctc
     5161 gcccgacggg caggagatgt ggggcaagtt cgtctaccgt gagatcgccg cgcccgagcg
     5221 catggtcttc gtcaattcct tttccgacgc gaagggcggg acgacccgcg cccccttcgc
     5281 cgccacctgg ccgctcgaag tgctgaacac gctgttaatc gccgagagcg gcggcaggac
     5341 gacactggcc ttgcgcggcg cgcccttgaa cgcgaccgat caggagcgcg cgacctttgc
     5401 cgcggcccag aaatcgatgc agcaaggctt cgccggcacc ttcgagcagc tcgatgccta
     5461 tctcgcaacc caaaggagca atcgccaatg accgccacag ccaacacccg ccgcgagaac
     5521 cccaaggtcg aacgatcgag ccgtcacggg atgttcgtcg tcgagcggcg ctacgacgcc
     5581 cggcccgcgc tcgtgtttgc cgcctgggcg tcgcccgagg ctaagtcgcg ctggttccac
     5641 ggcccccgtg gcgaatggac ggagaacttg cgcgagctcg attttcgcgt cggcgggcgc
     5701 gaacgcctgc tcggcacgcg catgagca
//
LOCUS       DASYNS010000022         8816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3281811, whole genome shotgun sequence.
ACCESSION   DASYNS010000022 DASYNS010000000
VERSION     DASYNS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3281811"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VGP50_00740"
     CDS             complement(<1..307)
                     /locus_tag="VGP50_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8025919.1"
                     /translation="MTARLTGRLALVTGASRGIGAAVARRFAAEGAHVILVARTTGGL
                     EERDDEIRALGSEATLVPLDLRDFDKIDQMGAALYQRFGRLDVLVGNAGVLGSLSPMG
                     "
     gene            complement(328..1782)
                     /gene="purF"
                     /locus_tag="VGP50_00745"
     CDS             complement(328..1782)
                     /gene="purF"
                     /locus_tag="VGP50_00745"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV8025920.1"
                     /translation="MRTTQPFDDDHLREECGVFGVWGHSEAAALVALGLHALQHRGQE
                     AAGIVTYDGKEFPAHRAMGLVGEQFSSKDVIARLKGHSAIGHVRYATTGEVAMRNVQP
                     LYNDFPFGGFAICHNGNLTNSYHLRRQLMRRGSLFHSTSDTEVINHLVATSLKPTTEE
                     RLVDALRQIEGAYSLAALTQEGIFGVRDPLGVRPLVLGKLGDAWILASETCALDIIGA
                     EFVRDIEPGELVTINALGVQSTKPFERRRSRFCVFEYIYFARPDSVIDGRNVYEARKR
                     IGAELARESPIAADIIVPVPDSGVPAAIGYAQESGLPFELGIIRNHYVGRTFIEPTDQ
                     IRHLGVKLKHNANRAKLAGQRVVLVDDSIVRGTTSTKIVEIVRQAGAREVHMRVSSPP
                     TSHSCFYGIATPERDKLLASRMDAAEMGRFIGADTLAFISIDGLYRAMGEAGRDPKAP
                     KFCDACFSGDYPIPLIDHEAGKVSTQLSLLAETA"
     gene            complement(1960..2625)
                     /locus_tag="VGP50_00750"
     CDS             complement(1960..2625)
                     /locus_tag="VGP50_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV8025921.1"
                     /translation="MNGLDIVVVVVILLSALFGFTRGLVKEALSIAAWVGAGFAAAYG
                     FAYVIPARLLPKGPVAAFIAVGVLFLVTLVILSFLASMMSSRVRHSTLSTLDRTLGLV
                     FGLVRGILLACLGYIALDWAMPAGGDRPAWIEEARTRTLLESGADRLRALVPRAYRDK
                     AVTAISDSKLTAEQIKEATGAIRALSTPRAPVTSGRENGSTYSPDDRHELDRLIQQRQ
                     DER"
     gene            complement(2622..4052)
                     /gene="radA"
                     /locus_tag="VGP50_00755"
     CDS             complement(2622..4052)
                     /gene="radA"
                     /locus_tag="VGP50_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV8025922.1"
                     /translation="MARAEARFVCQQCGAAHPKWAGRCDACGGWNCLVEEVARESAPK
                     GLGRAKGRKLAFVGLEGAAQAMPRRNSGIAEFDRVCGGGMVPGSALLVGGDPGIGKST
                     VLLQVTAALASGRAGKRLDCAYISGEESLDQVRLRAQRLGVADAPVQLAAATSIRDIV
                     ASLEEGSTPDVVVVDSIQTMYLDTLDSAPGTVSQVRASAQELIRLAKQRGFCVILVGH
                     VTKEGLIAGPRVLEHMVDTVLYFEGERGHQFRILRAVKNRFGPTDEIGVFEMTDAGLS
                     EVPNPSALFLAERQGDVSGAAVFAGMEGTRPLLVEIQALVAPSSLGTPRRAVVGWDGS
                     RLAMVLAVLEARCGIEIGVRDIYLNVAGGLRIAEPAADLAVAAALVSALTLEPVPAET
                     VIFGEIGLSGEVRPVGQAEARLKEAGKLGFTQAFLPPQRGRGKERPAAGGLALREVRH
                     LRQLVESFETTPQRAPRNVATPGARR"
     gene            complement(4071..4847)
                     /locus_tag="VGP50_00760"
     CDS             complement(4071..4847)
                     /locus_tag="VGP50_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8025923.1"
                     /translation="MANAPKIAVRGLKKSFGPKVVLDGVDLDVGVGESVVVIGGSGTG
                     KSVMVKCIIGLLDPDGGSIKIDGQELTSRSEAEREKRMQKFGMLFQGAALFDSLSVWE
                     NVAFGLIQGRDLDRRRAKEIAIAKLGAVGLTPDVGELRPSELSGGMQKRVGLARAIAA
                     EPEVIIFDEPTTGLDPIMADIINDLIIKCVRDLGATAISITHDMASARKIADRIAMIY
                     KGRIIWDGSKDLIDRSGNPYVDQFIHGRAEGPIKMEVRAL"
     gene            complement(4865..5635)
                     /locus_tag="VGP50_00765"
     CDS             complement(4865..5635)
                     /locus_tag="VGP50_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8025924.1"
                     /translation="MLDFLASIGRVFFLFLAATGRLVLFAIDAVTHAFRPPFYLRLIL
                     RQIVDIGYYSLPVVGLTAIFTGMVLALQTHTGFSRFSAEGAIATVVVLSMTRELGPVI
                     ASLMVAGRVGAAIAAEIGTMRVTDQIDALSTLSTNPFKYLVVPRLLAGTITVPALVLV
                     ADVIGVFGGFLVATSRFGFNPSAYIGQTSDYLQREDVMSGLVKAAVFGFIIALMGCYH
                     GYHSRGGAEGVGAATTHAVVSASIMILLSDYLLSSVLF"
     gene            complement(5638..6762)
                     /gene="alr"
                     /locus_tag="VGP50_00770"
     CDS             complement(5638..6762)
                     /gene="alr"
                     /locus_tag="VGP50_00770"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV8025925.1"
                     /translation="MSRADRAARVPARAGTVLDIDLAAIAGNWRRLQAELQPPALAAA
                     VVKADAYGLGMADVAPALATAGCRLFFVATLDEGIALRRVLPKAEIAVLNGLLPDTGG
                     EFRQARLLPVLNDLGQIAEWREDAATRRLPAVIHVDTGMARLGLPPDELGRLAAEPEL
                     LAGIPLAAVMSHLACADEKQHPLNAQQLGDFRRAIAQLPRAPASLAASSGIFLGRDYH
                     FDIARPGAALYGVNPTPGRPNPMAQVIRLKGRILQVRDVDRGTPVGYGAAQRMERPAR
                     LATIAIGYADGWLRSSSHRGRVSISGQPASVIGRISMDLLTVDVTGIDPALACAGAFV
                     DLIDEQHGVDAVAAAAGTIGYEILTALGRRHHRIYRGGAG"
     gene            complement(6749..8263)
                     /locus_tag="VGP50_00775"
     CDS             complement(6749..8263)
                     /locus_tag="VGP50_00775"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV8025926.1"
                     /translation="MENAASSKVTPLREGDGSYRAPPQNIEAEQALLAAILVNNAAYH
                     RVSDFLLAEHFALGVHGRIYAAVTRLIERGQIANPVTLKGLFDQDEALAEIGGAQYLA
                     RLATSVVTIINAEDYGRTIHDLYVRRQLIAIGEDTVNCAYKHDLEDDAARQIEAAETK
                     LFDLATTGQHEGSFQPFKMALTKAITLAEAAFKRAGRTTGVSTGFTDLDKLLGGLHPS
                     DLVILAGRPSMGKTALATNIGFHAADSYRGAKAPDGRTIAEDGAVVGFFSLEMSAEQL
                     ATRILSEESAVGSDRIRRGDVSHGDFERFVQASQRLASVPFFIDDTPALTIGALRTRA
                     RRLMRQQGLGLIVVDYLQLLRGGDTENRVQEISEITRGLKALAKDLNVPVLALSQLSR
                     AVEQREDKRPMLSDLRESGSIEQDADVVMFVFREEYYLARKEPTKIEHDDDRKFNEAY
                     EKWANRLQEVHGTAEIIIGKQRHGPVGKVTLRFDGETTRFDNFAPGDRLPDVTR"
     gene            8441..>8816
                     /locus_tag="VGP50_00780"
     CDS             8441..>8816
                     /locus_tag="VGP50_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025927.1"
                     /translation="MKGLLSINGVLRTLTAVMAAGLVITFAFEAWRAWNQQATAQQVV
                     TVVAMSRDLFDGLQSLRIERGTVNTALDTADVPSAQTLQEITRLRQLSDQSLDAAEAE
                     LTAAALPDGAEAGGNLHRAREGV"
BASE COUNT         1507 a         2876 c         3047 g         1386 t
ORIGIN      
        1 ggcccatggg cgacagcgag ccgagcacgc cggcgttgcc gacgagcacg tcgaggcggc
       61 cgaagcgctg atagagcgcc gcgcccatct gatcgatctt gtcgaaatcg cgcagatcaa
      121 gcggcaccag cgtcgcttca ctgccgagcg cgcggatctc gtcgtcgcgc tcttcgaggc
      181 cgcccgtagt gcgcgcgacc aggatcacat gcgcgccctc ggccgcgaag cgccgcgcca
      241 ccgccgcgcc gatgccgcgc gaagcgccgg tgacgagggc gaggcggccc gtgaggcgag
      301 ccgtcatggc tgaagcacga caggctctta cgccgtctcg gcgaggagcg agagctgcgt
      361 cgagactttg ccggcctcgt ggtcgatgag agggatcgga taatcgccgc tgaagcaggc
      421 gtcgcagaat ttgggtgcct tgggatcgcg tccggcttcg cccatggcac ggtagaggcc
      481 gtcgatcgag atgaaagcga gggtgtccgc gccgatgaag cggcccatct cggccgcgtc
      541 catgcgcgac gccagcagct tgtcgcgctc gggcgtggcg atgccgtaga agcaggagtg
      601 gctggtgggc gggctcgaga cgcgcatatg cacttcgcgc gcgccggcct gtcgcacgat
      661 ttcgacgatc ttggtcgagg tcgtaccgcg cacgatcgag tcgtcgacca gcaccacgcg
      721 ctggccggcg agtttcgcgc ggttggcgtt gtgcttgagc ttgacgccga gatgacggat
      781 ctggtcggtc ggctcgatga acgtgcggcc gacatagtgg ttgcgaatga tgccgagctc
      841 gaaaggaagt ccgctctcct gcgcatagcc gatcgccgcc gggacaccgg aatcgggcac
      901 cggcacgatg atgtcggcgg cgatggggct ctcacgcgcc agctccgcgc cgatgcgctt
      961 tctcgcctcg tagacgttgc gcccgtcgat cacgctgtcc ggccgcgcga agtagatgta
     1021 ttcgaagacg cagaagcgac tgcgccggcg ctcgaagggc ttcgtgctct gcacgcccag
     1081 cgcattgatg gtgacgagct cgcccggctc gatgtcgcgc acgaactcgg cgccgatgat
     1141 gtcgagggcg caggtctcgg acgcgagaat ccaggcatcg ccgagcttgc ccaggacgag
     1201 cggccgcacc cccaaggggt cgcgcacgcc gaagatgccc tcttgcgtga gcgcggccag
     1261 cgaataggcg ccctcgatct ggcgcagcgc atcgacgagc cgctcctcgg tcgtgggctt
     1321 caggctcgtg gcgaccagat gattgatgac ctcggtgtcg ctcgtcgagt gaaagaggct
     1381 gccgcgacgc atgagctgac gccgcaaatg gtaggaattg gtgaggttgc cgttgtggca
     1441 gatggcgaat ccgccgaagg ggaagtcgtt gtagagcggc tgcacattgc gcatcgccac
     1501 ttcgccggtg gtggcatagc ggacatgacc gatggcggaa tggcccttga ggcgcgcaat
     1561 cacgtccttc gagctgaatt gctcgcccac gagacccatg gcgcgatggg cggggaattc
     1621 cttgccgtca taggtgacga tgccggccgc ctcttgtccc cggtgctgga gggcatggag
     1681 ccccaaggcc acgagcgccg ccgcctccga atgtccccag acgccgaaaa cgccacattc
     1741 ctcgcgcagg tgatcgtcgt cgaagggctg cgtcgtccgc atgaagcgcc ttggttaaag
     1801 gaccgattcg ccgttccggc tcgccctaga ggtaagcatt gcggcgcacg atgtcgagcg
     1861 catggccagg gttctacacg catcggccag cgactgccag ggacttcgca cggcgccggg
     1921 atttctcata cgcgcatgtc caaaggagag aaatcccggc tagcgctcgt cctgccgttg
     1981 ctggatgagg cggtcgagtt cgtggcgatc gtcgggcgag taggtggagc cgttttcgcg
     2041 cccggacgtg accggcgccc tgggcgtgct gagtgcccga atcgccccgg tcgcctcctt
     2101 gatctgctcg gccgtcaact tgctgtcgct gatggcggtc acggccttat cgcgataggc
     2161 gcggggtacc agagcgcgca accggtcggc accgctctcc agcagggtgc gtgtgcgcgc
     2221 ttcctcgatc cacgccgggc gatcaccgcc ggccggcatc gcccagtcca gagcgatata
     2281 gccgagacag gccagcaaga tgccgcgcac cagcccgaag accaggccca aggtgcggtc
     2341 gagcgtggag agcgtgctgt gccgcacccg cgacgacatc atcgaggcca gaaaggacag
     2401 gatgacgagc gtgaccagga acagcacgcc gacggcaatg aacgccgcga ccgggccttt
     2461 gggcagcaag cgggccggaa tcacataggc gaagccgtaa gccgcggcga atccggcgcc
     2521 gacccatgcc gcgatcgaca gcgcttcctt gacgagaccg cgggtgaagc cgaaaagcgc
     2581 cgacagcaga atcaccacga cgaccacgat gtcgaggccg ttcatcggcg cgctcctggt
     2641 gtcgcgacgt tgcgcggcgc gcgctgcggt gtggtctcga aggattcgac gagttgacgc
     2701 agatggcgga cctcgcgcaa ggcgagtccg cccgccgccg gccgctcctt gccgcgaccg
     2761 cgttgcggcg gcaggaaggc ctgggtgaag ccgagcttgc cggcttcctt gagccgcgcc
     2821 tcggcttgcc cgacggggcg cacttctccc gacaggccga tctcgccgaa aatcaccgtc
     2881 tccgccggca ccggctccag cgtcagcgcc gagacgagcg ccgccgccac cgccagatcg
     2941 gcggcgggct cggcgatgcg caggccgccc gcgacattga gatagatgtc gcgaacgccg
     3001 atctcgatgc cgcagcgcgc ctcgagcacg gccagcacca tggcgaggcg gctgccgtcc
     3061 cagccgacga cggcacgccg cggcgtgccc aaggaggacg gcgccaccag cgcctggatc
     3121 tcgacgagga gcggccgggt cccttccatg ccggcgaaga ccgcggcacc cgagacgtcg
     3181 ccttggcgct cggcgagaaa cagcgccgac gggttcggca cttcgctcaa gcccgcatcg
     3241 gtcatctcga agacgccgat ctcgtcggtc ggaccgaagc ggttcttgac cgcgcgcagg
     3301 atgcggaatt gatggccgcg ctcgccttcg aaatagagga cggtgtcgac catgtgctcg
     3361 aggacgcgcg gcccggcgat gaggccttcc ttggtgacat ggccgacgag gatgacgcag
     3421 aagccgcgtt gcttggcgag gcgaatcagt tcctgcgccg aggcgcgcac ctggctcacc
     3481 gtgccggggg cgctgtcgag cgtgtcgaga tacatggtct gaatcgaatc gaccaccacg
     3541 acgtcgggtg tgctgccttc ctcgagcgaa gcgacgatgt cgcggatgct ggtggcggcc
     3601 gcgagctgca ccggcgcgtc cgcgacaccg agtcgctggg cgcgcagccg cacctgatcg
     3661 agggattcct cgcccgagat ataggcgcaa tcgagtcgct tgccggcccg gcccgaagcc
     3721 agcgccgccg ttacctggag gagcaccgtg gatttgccga tgccgggatc gccccccacc
     3781 agcagcgcgg agcccggcac catgccgccg ccgcagacgc gatcgaactc ggcgatgccg
     3841 ctgttgcggc gcggcatcgc ctgagccgcg ccctcgaggc cgacgaaagc gagcttccgg
     3901 cccttggcgc ggccgagacc cttgggcgcg ctctcccgcg ccacttcttc gaccaggcag
     3961 ttccagccgc cgcaggcatc gcaccgtccg gcccatttgg gatgagccgc accgcattgc
     4021 tggcagacga accgggcctc agcgcgcgcc atcagtcggt cctcgaccac tcacaaggcg
     4081 cgcacttcca tcttgatcgg gccttcggcg cggccgtgaa tgaactggtc gacataaggg
     4141 ttgccggagc gatcgatcag gtccttggag ccatcccaga taatacgccc tttgtagatc
     4201 atggcgatac ggtcggcgat cttgcgcgcg ctcgccatgt catgggtgat cgagatggcg
     4261 gtggcgccca ggtcgcgcac gcatttgatg atgaggtcgt tgatgatgtc ggccatgatc
     4321 gggtcgagcc cggtggtcgg ctcgtcgaag atgatgacct cgggctcggc ggcgatggcg
     4381 cgcgccagcc ccacgcgctt ctgcatgccg cccgacagct ccgacggccg gagctcgccg
     4441 acatcgggcg tcaggccgac ggcgcccagc ttggcgatgg cgatctcctt cgcccggcga
     4501 cgatcgaggt cgcgcccctg gatcagcccg aacgccacat tctcccagac cgagagcgag
     4561 tcgaagagcg ccgccccctg gaacagcatg ccgaatttct gcatgcgctt ctcgcgctcg
     4621 gcctccgacc ggctcgtcag ctcctggccg tcgatcttga tcgagccgcc atcggggtcg
     4681 agcaggccga tgatgcattt caccatcacc gacttgccgg tgcccgagcc gccgatcacg
     4741 acgacgctct cgccgacgcc gacatcgaga tcgaccccgt cgagcacgac cttcggcccg
     4801 aaggacttct tgaggccgcg cacggcgatt ttcggagcgt tggccatttt cccggcgccg
     4861 gctcctagaa caggacgctg ctgagcaggt agtcggagag caggatcatg atcgaggccg
     4921 agaccacggc atgggtcgtc gccgcgccca cgccctcggc gccgccgcgc gaatgatagc
     4981 cgtgatagca gcccatgagt gcgatgataa agccgaagac cgccgccttg acgagccccg
     5041 acatgacgtc ctcgcgctgg agatagtcgc tcgtctgtcc gatataggcc gacggattga
     5101 agccgaatcg ggatgtggcg acgagaaagc cgccgaagac accgatgacg tccgccacca
     5161 gcaccagcgc cggcaccgtg atggtgcccg cgagcagccg cggcacgacc aggtacttga
     5221 acgggttggt cgagagcgtc gacagcgcgt cgatctggtc ggtgacgcgc atggtgccga
     5281 tctcggcggc gatcgccgcg ccgacgcggc ccgccaccat gagcgaggcg atcaccggac
     5341 cgagttcgcg cgtcatcgac agcacgacca cggtggcgat ggcgccctcg gcggagaatc
     5401 gactgaagcc ggtgtgggtc tggagcgcca gcaccatgcc ggtgaagatc gccgtcagcc
     5461 cgaccaccgg cagcgagtaa tagccgatgt cgacgatctg gcggaggatg aggcgcagat
     5521 agaaaggcgg gcggaaggca tgggtgacgg catcgatcgc gaagagcacg agacggccgg
     5581 tcgccgccag gaacaggaag aagacgcggc cgatgctggc gaggaaatcg agcatgctca
     5641 gccggccccg ccgcgataga tgcgatggtg gcggcggccg agcgcggtca ggatctcgta
     5701 gccgatggtt ccggcggcgg cggccacggc atcgacgccg tgctgttcgt cgatgagatc
     5761 gacgaacgct ccggcgcacg caagcgcagg atcgatgccc gtcacgtcga ccgtcagaag
     5821 gtccatcgag atgcgtccga tgacggaagc tggctgaccg gagatgctga cacggccgcg
     5881 gtgacttgat gaacgcaacc aaccatcggc gtaaccaatg gcgattgtgg cgaggcgggc
     5941 agggcgctcc atgcgctgcg cggcaccata gccaacgggt gtacccctgt caacgtcgcg
     6001 cacttgcaag attcgccctt tgaggcgaat gacttgcgcc atcgggttgg gtcgcccggg
     6061 tgtcggattc acgccataga gtgcagcgcc cggacgcgcg atgtcgaaat ggtagtcgcg
     6121 cccgaggaaa atgccggaag aggccgcgag actcgccggc gcgcgcggca gctgagcgat
     6181 cgcgcggcga aaatcgccaa gctgctgcgc gttgagcgga tgctgcttct cgtcggcgca
     6241 agcgagatgg ctcatcaccg cggcgagcgg gatgccggcg agcagttccg gttccgcagc
     6301 gagacggccg agctcatcgg gcgggagccc gagccgcgcc atgccggtgt cgacatggat
     6361 gaccgcgggc aagcggcgcg tcgcggcatc ttcacgccat tctgcgatct ggccgagatc
     6421 gttgaggacc gggagcagcc gcgcctggcg gaattcgccg ccggtgtccg ggagcagtcc
     6481 gttgaggacc gcaatctctg ccttgggcag gacgcggcgc aaagcgatgc cctcatcgag
     6541 cgtggcgacg aagaagaggc ggcagccggc cgtagcgagc gcgggcgcga cgtcggccat
     6601 tcccagtccg taggcgtcgg ccttgacgac cgcggcagcc agcgccggcg gctgcagctc
     6661 cgcctgaagc cgccgccagt tgccggcgat cgccgcgaga tcgatgtcga gcaccgtgcc
     6721 ggcgcgggcc ggcacgcgcg ccgcacgatc agcgcgtgac atcggggagg cggtcgcccg
     6781 gcgcgaaatt gtcgaatcgg gtggtctcgc cgtcgaatcg gagagtcacc ttgccgaccg
     6841 ggccgtggcg ctgcttgccg atgatgatct cggcggtgcc gtgcacctcc tggagccggt
     6901 ttgcccattt ctcataggct tcgttgaatt tgcggtcgtc gtcatgctcg atcttcgtcg
     6961 gctctttgcg cgccagataa tattcttcgc ggaagacgaa catcacgacg tccgcgtcct
     7021 gctcgatcga gcccgactcg cggagatccg acagcatcgg acgcttgtcc tcgcgctgct
     7081 cgacggcgcg cgagagctgc gacagcgcga gcacgggcac attcaggtcc ttggcgagcg
     7141 ccttcaagcc gcgggtgatc tccgagatct cctgcacgcg gttctcggtg tcgccgccgc
     7201 gcagcagctg cagatagtcg acgacgatga gcccgagacc ctgctggcgc atcagccggc
     7261 gcgcgcgggt gcgcaaggcg ccgatggtga gcgccggcgt atcatcaatg aagaagggca
     7321 ccgacgccag tcgctggctc gcctgaacga agcgctcgaa atccccatgc gacacgtcgc
     7381 cgcggcggat gcggtccgag cccaccgccg attcctccga aagaatgcgc gtcgcgagct
     7441 gctcggccga catctcgagg gagaagaatc cgacgacggc gccgtcctcg gcaatggttc
     7501 gtccatccgg cgccttggcg ccgcgatagg agtcggcggc gtgaaatccg atattcgtcg
     7561 ccagcgccgt cttgcccatg gacggccgtc ccgcaaggat gacgagatcc gacgggtgca
     7621 gcccgcccag gagcttgtcg agatcggtga agccggtcga gacgccggtg gtgcggccgg
     7681 cgcgcttgaa ggccgcctcg gcgagggtga tcgccttggt cagcgccatc ttgaacggct
     7741 gaaagctgcc ctcgtgctgg cccgtcgtcg cgagatcgaa gagcttcgtc tcggcggcct
     7801 cgatctgccg cgcggcatcg tcctcgaggt catgcttgta ggcgcagttg acggtgtcct
     7861 cgccgatcgc gatgagctgg cgccgtacat agaggtcatg gatggtgcgg ccgtagtcct
     7921 cggcgttgat gatcgtcacc acggacgtcg cgagtcgcgc cagatattgc gcgccgccga
     7981 tctcggcaag cgcttcgtcc tgatcgaaca agcccttgag cgtcaccggg ttggcgatct
     8041 ggccgcgctc gatgagccgc gtgaccgcgg catagatgcg gccgtgcacg cccagcgcga
     8101 aatgctcggc cagcaggaag tccgagaccc ggtgataggc cgcgttgttc accaggatgg
     8161 cggcgaggag cgcttgctcc gcctcgatat tctgcggcgg cgcgcgatag ctcccgtcac
     8221 cctcgcgcag cggggtgacc ttcgacgatg cggcattttc catggcgcga atgttaccaa
     8281 ttcgttatcg cccgacgcac gcggaaatcg acgcacaggc tgtggaaggc ggggacgggg
     8341 ccccggtcac ggccggtgct cggtcaaata cagcagaaat taagcctttg gttacgcggg
     8401 ttacggtatc aagcggttct gctcggaggg aagtgccggc atgaagggtc ttttgtcgat
     8461 caatggcgtg ctgcgcacgc tgactgccgt gatggccgcc ggtctggtca tcactttcgc
     8521 cttcgaggcg tggcgcgcct ggaatcagca ggcgacggcg cagcaggtgg tcacggtcgt
     8581 cgccatgtcg cgcgacctct tcgacggctt gcaaagcctc cggatcgagc gcggcacggt
     8641 caataccgct ctcgacaccg ccgacgtccc gagcgcccaa acgctgcaag agatcacgcg
     8701 gctgcgccag ttgtccgacc agtcgctcga cgcggcggaa gcggagctga cggccgcggc
     8761 gctgcccgac ggggcggaag cgggcggcaa tctgcaccgc gcccgcgaag gtgtca
//
LOCUS       DASYNS010000023         2376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1878315, whole genome shotgun sequence.
ACCESSION   DASYNS010000023 DASYNS010000000
VERSION     DASYNS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878315"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VGP50_00785"
     CDS             complement(<1..374)
                     /locus_tag="VGP50_00785"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEV8025928.1"
                     /translation="MKRAFVLASLLMTAAALTAHAGEGRSAPVVVELFTSQGCSTCPP
                     ADAYLGELASRPGVIALAFHVDYWNYIGWTDPFASKAATDRQRSYAKQLGLRYVYTPQ
                     MVINGTMEGVGSGREAITQLIA"
     gene            complement(580..>2376)
                     /gene="acnA"
                     /locus_tag="VGP50_00790"
     CDS             complement(580..>2376)
                     /gene="acnA"
                     /locus_tag="VGP50_00790"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEV8025929.1"
                     /translation="LADRATIGNMAPEYGATCGFFPIDRVTINYLTFTGRDPERAKLV
                     EAYAKAQGLWHDEKAPEPVFTDTLSLDLATVEPSLAGPRRPQDRVALSGVAKSFAAEL
                     PKLAGAASGSQKPVPVAGANYALEPGAVVIAAITSCTNTSNPSVMLAAGLLARNAVQR
                     GLKVKPWVKTSLAPGSQVVTDYYAAAGLQQDLDALGFNLVGYGCTTCIGNSGPLPDPI
                     AEAVEKGDLVVAAVLSGNRNFEGRISPHVRANYLASPPLVVAYALAGNMNVDLTQDPL
                     GEGKDGKPVYLRDIWPSSQEVLGTIGKSLTPDMFRNRYGNVFEGPQAWRKVTVSTGTT
                     FTWDMGSTYVAHPPYFENMPKEPAPLADVTMARPLAILGDSITTDHISPAGSIKKDSP
                     AGQYLVSHQVRPADFNSYGARRGHHEVMMRGTFANIRIRNEMVPGVEGGMTKHLPDNQ
                     IMPIYDAAMLYQKEGVPLVIIAGKEYGTGSSRDWAAKGTRLLGVRAVIAESFERIHRS
                     NLVGMGVLPLQFKDGATRQTLKLDGSERFDILGVAQGLKPRSEIACRIHRAAGTVESL
                     SLHCRIDTLDELEYFRNGGILQYVLRHMMKTA"
BASE COUNT          409 a          750 c          800 g          417 t
ORIGIN      
        1 tcggcgatga gctgggtgat cgcttcacgc cccgagccca cgccctccat cgtgccgttg
       61 atcaccatct gcggcgtgta gacgtaacgc aagccaagct gcttcgcata gctgcgctgg
      121 cgatccgtgg ccgctttcga agcgaaagga tcggtccagc cgatgtagtt ccaatagtcg
      181 acatggaagg cgagcgcgat cacaccggga cgcgaggcaa gctcgcccag ataggcgtcc
      241 gccggcgggc aggtgctgca gccctgcgag gtgaacagct cgaccaccac gggcgccgac
      301 cggccctcac cggcatgcgc ggtgagcgcc gccgccgtca tcagcagtga cgcgagaacg
      361 aacgctcgct tcatagaagt acccaactga gcatttgata atgatgcgcc agcactcttc
      421 acggcgccag tcaaacacct tcacaatagg gtgaacaccc caaccattga aggctaaaac
      481 atttgccgct ttcgggaaaa atgcggcgga aaccttcgct gcgacgcggc tgcgagaagt
      541 gcgagaagag tgaatatccc ctcgcgttct ctcgtccggc tacgccgttt tcatcatgtg
      601 gcgcagcaca tattgaagaa taccgccgtt acggaagtat tcgagctcgt cgagcgtgtc
      661 gatgcggcag tgcagcgaca atgattcgac cgtgccggcg gcgcgatgga tgcggcaggc
      721 gatctcgctg cgcggcttca agccctgggc gacgccgaga atgtcgaagc gctcgctgcc
      781 gtcgagcttc agcgtctgac gcgtcgcgcc gtccttgaac tggagcggca gcacgcccat
      841 gccgacgagg ttggaccggt gtatgcgctc gaagctctcg gcgatcacgg cgcggacgcc
      901 gaggaggcgc gttcctttgg ccgcccagtc gcgcgacgag cccgtgccgt attccttgcc
      961 ggcgatgatc acgagcggca cgccttcctt ctggtagagc atcgccgcgt catagatcgg
     1021 catgatctga ttgtccggca ggtgcttggt catgccgcct tcgacacccg gcaccatctc
     1081 gttgcggatg cggatattgg cgaaggtgcc gcgcatcatc acttcgtgat ggccgcgcct
     1141 cgccccatag gaattgaaat cggccgggcg cacctgatgg ctgacgaggt attggccggc
     1201 ggggctgtcc ttcttgatcg agcccgcagg cgagatgtgg tcggtcgtga tgctgtcgcc
     1261 gaggatcgcc aacggccgcg ccatcgtcac gtccgcgagc ggcgccggct ctttcggcat
     1321 gttctcgaaa tagggtggat gcgcgacata ggtggagccc atgtcccagg tgaaggtcgt
     1381 gccggtcgac accgtcacct tgcgccacgc ctgcggcccc tcgaagacgt tgccgtagcg
     1441 gttgcggaac atgtcgggcg tgagcgactt gccgatcgtg cccagcacct cctggctcga
     1501 cggccagatg tcgcgcagat agaccggctt gccgtccttg ccttcgccga gcggatcctg
     1561 ggtgaggtcg acattcatgt tgccggcgag cgcataggcg acgacgagcg gcggcgacgc
     1621 cagatagttg gcgcgcacat gcggactgat gcggccttcg aaattgcggt tgccggagag
     1681 cactgccgcc accacgagat cgcccttctc gacggcctcg gcgatgggat cgggcagcgg
     1741 cccagaattg ccgatgcagg tggtgcagcc gtagcccacg agattgaaac cgagtgcatc
     1801 gagatcctgc tgcagcccgg ccgccgcata gtagtcggtc acgacctggc tcccgggcgc
     1861 gagcgaggtc ttgacccagg gcttcacctt gaggccgcgc tggaccgcgt tccgcgccag
     1921 gaggcccgcg gcgagcatca cgctcgggtt cgaggtgttg gtgcagctgg tgatggcggc
     1981 gatgacgacg gcgccgggct cgagcgcgta attcgcgccc gcgacgggca ccggcttctg
     2041 gctgccgctg gcggcgccgg cgagcttcgg cagctcggcg gcgaaagatt tagcgacgcc
     2101 cgacagcgcc acgcgatcct gcggccggcg cggaccggcg agcgacggct cgaccgtcgc
     2161 cagatcgagc gacagcgtgt cggtgaagac cggctcgggc gccttctcgt catgccatag
     2221 gccttgcgcc ttggcatagg cctcgacgag cttcgcgcgc tcggggtcgc ggccggtgaa
     2281 ggtgaggtag ttgatggtca cgcggtcgat cggaaagaag ccgcacgtcg cgccatattc
     2341 cggcgccatg ttgccgatgg tcgcgcggtc ggcgag
//
LOCUS       DASYNS010000024         4191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_631711, whole genome shotgun sequence.
ACCESSION   DASYNS010000024 DASYNS010000000
VERSION     DASYNS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631711"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..779)
                     /locus_tag="VGP50_00795"
     CDS             complement(360..779)
                     /locus_tag="VGP50_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HEV8025930.1"
                     /translation="MALHLIKLAVGAEDVKQLRQFQKERRRERGRYVFYTRHLPRRRE
                     EIVDGGSIYWVIKGYVRARQRILEFIPVVEEDGERYCLVRYDRTLVDTVWQPKRPFQG
                     WRYLQPKDAPPDRPAYAAADDELPPAIAQELRSLGLL"
     gene            complement(790..1698)
                     /locus_tag="VGP50_00800"
     CDS             complement(790..1698)
                     /locus_tag="VGP50_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEV8025931.1"
                     /translation="MRLPKAVRMVEVGPRDGLQNEAQVLPAATKVALIEQLVDAGLTV
                     VEAGSFVSPKWIPQMADTAAVLAMLKRKPGVSYPVLVPNIKGLDAAREAGVTDIAIFA
                     AASESFSRRNINCSIDESFERFAPVAEAAKRDGMRMRGYISCVVDCPYDGPVAPVAVA
                     DVAARLLALGCTEISLGETIGTGTPARIEAMIEAVASRVPVERLAVHFHDTYGQALAN
                     ILASLEKGIAVVDSSVAGLGGCPYAKGASGNVASEDVLYMLSGLGIATGVDLGKLAAS
                     GRFISQVLGREPVSKAARALAAKAAA"
     gene            complement(1702..3708)
                     /locus_tag="VGP50_00805"
     CDS             complement(1702..3708)
                     /locus_tag="VGP50_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEV8025932.1"
                     /translation="MFAKILIANRGEIACRVIRTAQRMGIRTVAVYSEADAGALHVAM
                     ADEAYPIGAAPARESYLEIDRIIETARRAGAEAVHPGYGFLSENAAFAEACEEAWIIF
                     IGPGAKAIRAMGDKAAAKTLMEKAGVPLVPGYHGAAQDEPHLAAEAQRIGYPVLIKAS
                     AGGGGKGMRVVETPERFVAALASAKREAASAFGDDRMLLEKYLTRPRHVEIQIFADFD
                     GHVVHLFERDCSIQRRHQKVIEEAPAPGLSAERRRAMSDAAVAAARAVGYVGAGTVEF
                     IVEGDNFYFIEMNTRLQVEHPVTEMITGLDLVEWQIRVAAGDPLPLGQAEIALRGHAV
                     EARLYAENPQRNFLPATGTLQRLRMPETSADLRIDTGVREGDAVTMYYDPMIAKVIAW
                     GEDRASALRRLASGLGKYRIAGLATNRDLLMRILRHQSFMVADFDTGFIERHAATLLP
                     PPSAAPRAALAAASLALLWDQAEAARAPDPSSTDLHSPWRRRDFWRLNGATHQDMSWR
                     DGDEEHLVRAHYHRDRYYLDIDGESSAVAASLAANGDLALDLDDIRVRLGVVRHGSEV
                     TVMGDDGTWRLTYVDPLALKTAEAVAGGRLTAPMPGKVMQVLAKAGVPVKRGDPLMVI
                     EAMKMEHTISAPADGVVERVNFRAGDFVAEGAELIAFAAAGEEA"
     gene            complement(3712..>4191)
                     /locus_tag="VGP50_00810"
     CDS             complement(3712..>4191)
                     /locus_tag="VGP50_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV8025933.1"
                     /translation="VARVNGAAFAGGLGLVSCCDIAVAAEEAVLSLSEARLGLVPATI
                     APYVVAAIGPRAARRYCLTAERFSASEALRLGLVHEVAPRASLDAAVERIVAALLEAA
                     PGAQARAKRLIAEVQSRPLGDATIALTAQVIADARASAEGREGLASFFEKRKPAWRA"
BASE COUNT          714 a         1473 c         1360 g          644 t
ORIGIN      
        1 ccaagtcctg gcaccgcacg atctcatctc gcgctcggaa ttgactgctc aattccttac
       61 ctaagatacg cagaccatgc gataccgatt aggcaccgcc gcctgcgcat cccttcctct
      121 tgccaacaat gtcaaagagc aacccgtaag ggacggcgct tataagcttg ctgcagaagc
      181 aagtcaacaa aaagcaccta cccttgtcgg gatcttccga tacggaagac gacgcacgcg
      241 ctgcgctctg cagcgttgga cgtcgaccgg tttcagaaga gccgccgaag cggccagcac
      301 cccgatatag gccgacggac gcttcttgtc cagaggcacc cgtcaaaaat ctgtctcgat
      361 caaagcagcc cgagcgaccg caactcctgc gcgatcgccg gcggcagctc gtcatccgca
      421 gcggcgtagg ccggacgatc gggcggcgcg tccttgggct gcagatagcg ccagccctgg
      481 aagggacgct tcggctgcca taccgtgtcg acgagggtgc ggtcgtagcg cacgagacaa
      541 tagcgctcgc cgtcctcctc gacgacggga atgaactcga ggatgcgctg gcgcgcccgc
      601 acataacctt tgatcaccca gtagatcgag ccgccatcca caatctcctc gcggcgccgc
      661 ggcaaatgtc gcgtatagaa gacgtagcgg ccgcgctcgc gccggcgctc cttctggaac
      721 tggcgcagct gcttcacatc ctcggcgccg acggcgagct tgatcagatg cagcgccatg
      781 ccgcgttcct cacgccgccg ctttggcggc gagcgcacgc gccgcctttg agacgggctc
      841 gcgtcccaac acttgcgaaa tgaagcggcc ggaagcggcg agtttgccca ggtcgacgcc
      901 ggtagcgatg ccgagaccgc tcaacatata gagcacgtcc tcgctcgcga cattgcccga
      961 ggcacccttc gcataaggac agccgccgag ccccgcaacc gagctgtcca ccacggcaat
     1021 ccccttctcg agcgacgcca ggatattcgc cagcgcctgg ccataggtgt cgtggaaatg
     1081 cacggccagg cgctcgaccg gcacgcgcga agcgaccgcc tcgatcatcg cctcgatgcg
     1141 cgccggcgtg ccggtgccga tggtctcgcc cagcgagatc tcggtgcagc cgagcgccag
     1201 gagccgcgcc gccacatccg cgactgccac cggcgcgacc ggcccgtcat aaggacaatc
     1261 gacgacgcag gagatataac cgcgcatccg catgccatcg cgtttggctg cttccgccac
     1321 cggtgcgaag cgctcgaagc tctcgtcgat cgagcagttg atgttgcggc gcgagaagct
     1381 ctccgacgcc gcggcgaaaa tggcaatgtc ggtgacgccc gcttcccgcg ccgcgtcgag
     1441 gcccttgatg ttcggcacga gcaccgggta gctcacgccc ggcttgcgct tcaacatcgc
     1501 caggaccgcg gcggtatcgg ccatctgcgg aatccatttg ggcgagacga agcttccggc
     1561 ttcgacgacg gtgagcccgg catcgacgag ctgctcgatg agcgcgacct tcgtcgctgc
     1621 cggcagcacc tgcgcctcgt tctggagtcc gtcgcgcggg ccgacctcga ccatccggac
     1681 ggctttggga agccgcatcg cttatgcctc ctcccccgcc gccgcgaatg cgatcagttc
     1741 ggcgccttcg gcgacgaaat cgccggcgcg gaagttgaca cgctcgacga cgccatcggc
     1801 cggcgccgag atcgtgtgct ccatcttcat cgcctcgatc accatgagcg ggtcgccgcg
     1861 tttgaccggg acgcccgcct tggccagcac ctgcatcacc ttgcccggca tcggcgcggt
     1921 gagccggcca cccgccacgg cttcggccgt cttgagcgcc agcggatcga cataagtgag
     1981 ccgccaggtc ccgtcatcgc ccatgacggt gacctcgctc ccatgccgca ccacgcccag
     2041 gcgcacgcgg atgtcgtcga gatcgagcgc cagatcgcca ttggcggcga gcgacgccgc
     2101 gaccgcgctg ctctcgccgt cgatgtcgag gtaatagcgg tcgcgatggt aatgggcgcg
     2161 cacgagatgc tcttcatcgc cgtcgcgcca gctcatgtcc tgatgagtcg caccgttgag
     2221 ccgccagaag tcgcgccggc gccagggcga gtggagatcg gtcgagctcg gatcgggagc
     2281 gcgcgccgct tccgcctggt cccacagcag agcgaggctc gccgcggcca gcgcggcgcg
     2341 gggcgcggcc gatggcggcg gcaacagcgt cgccgcatgg cgctcgatga agccggtatc
     2401 gaaatcggcg accatgaagg attgatggcg caggatgcgc atcagcagat cgcggttggt
     2461 cgcgaggccg gcgatacgat acttgccgag gcccgaggca agccggcgca gcgccgacgc
     2521 ccgatcctcg ccccaggcga tcaccttggc gatcatcggg tcgtaataca tcgtgacggc
     2581 gtcgccttcg cgcacgcccg tgtcgatgcg cagatcggcg ctcgtctcgg gcatgcgcag
     2641 ccgctgcagc gtgcccgtcg ccggcaggaa attgcgttgg ggattctcgg cgtagagccg
     2701 cgcttcgacg gcatggccgc ggagcgcgat ctccgcctgg cccagcggca acgggtcgcc
     2761 ggcggcgacg cggatctgcc attcgacgag atcgaggccg gtgatcatct ccgtcacggg
     2821 atgctcgacc tggagccgcg tgttcatctc gatgaagtag aaattgtcgc cctcgacgat
     2881 gaactcgacc gtccccgcac cgacatagcc gacggcgcgt gccgcggcga ccgccgcatc
     2941 actcatggcg cggcgccgct cggcgctcaa gcccggcgcc ggcgcttcct cgatgacttt
     3001 ctggtggcgg cgctggatcg agcagtcgcg ctcgaagaga tgcacgacgt gaccgtcgaa
     3061 atcggcgaag atctggatct cgacatggcg cggccgcgtc aggtatttct cgagcagcat
     3121 gcggtcgtcg ccgaaggccg acgccgcttc gcgcttcgcc gaagcgagcg ccgcgacaaa
     3181 ccgctcgggc gtctcgacga cgcgcatgcc tttgccgcca ccgccggccg acgccttgat
     3241 gagcacggga taaccgatgc gctgcgcttc cgcggcgaga tgcggctcat cctgcgccgc
     3301 gccgtgatag cccggaacca gcggcacgcc ggctttctcc atcagcgttt tcgccgcggc
     3361 cttgtcgccc atggcgcgga tcgccttcgc gcccgggccg atgaagatga tccacgcttc
     3421 ctcgcaggcc tcggcgaagg cggcgttctc cgagagaaaa ccatagccgg gatgcacggc
     3481 ttcggcgccg gcgcggcgcg ccgtctcgat gatgcggtcg atctcgagat agctctcgcg
     3541 cgccggcgca gcgccgatcg gataggcctc atcggccatg gcgacatgga gcgcgccggc
     3601 atcggcctcg gaatagacgg ccaccgtgcg gatgcccatg cgctgcgcgg tgcggatgac
     3661 ccggcaggcg atctcgccgc gattggcgat gaggatcttc gcgaacatcg ctcaggcccg
     3721 ccaggccggc ttgcgcttct cgaagaagga agcgaggcct tcgcgtccct cggccgacgc
     3781 gcgcgcgtcg gcgatgacct gtgcggtgag cgcgatggtc gcatcgccga gcggacggct
     3841 ctgcacttcg gcgatgagac gcttggcgcg cgcctgcgcg cccggcgccg cctcgagcag
     3901 cgccgcgacg atcctctcaa ccgctgcatc gagcgatgcg cgcggcgcga cctcgtgcac
     3961 gaggccaagg cgcagcgctt cgctcgccga gaaacgctcg gcagtgaggc aatagcgccg
     4021 cgccgcccgc ggcccgatcg ccgccacgac ataaggcgcg atggtcgccg gcaccaaccc
     4081 gagccgcgcc tccgatagcg acaacacggc ctcttcggcg gcgacggcga tatcgcaaca
     4141 ggagacgaga ccgagaccgc cggcaaaggc cgcgccgttg acgcgggcca c
//
LOCUS       DASYNS010000025         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3128502, whole genome shotgun sequence.
ACCESSION   DASYNS010000025 DASYNS010000000
VERSION     DASYNS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3128502"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VGP50_00815"
     CDS             complement(<1..200)
                     /locus_tag="VGP50_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025934.1"
                     /translation="MRILIVIPHFFRAIDPQATNRSHRGDARDERLRALIATVAALHQ
                     AFGAGVYGLDHFNRAAWQAEGAG"
     gene            518..2659
                     /locus_tag="VGP50_00820"
     CDS             518..2659
                     /locus_tag="VGP50_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEV8025935.1"
                     /translation="MPLELAPAEAAADGAAALVLLLRFHGVAADPAQIRHRFGGAAIG
                     LPEMVRCARELKLRARAVHSDWERLAKTPLPAIAERRDGGFLILAKLSNEGVLVQDPG
                     AGRPQVLPRAEFEQQWSGRLLLMTRRASLSDLARRFDVTWFLQAMHKYRRLLAEVLVA
                     SFFLQLFALVTPLFFQVVIDKVLVHRGLTTLDVLIVGLVTVSLFESVLGALRTYVFSH
                     TTNRIDVELGARLFRHLLALPIAYFNARRAGDSVARVRELENIRNFLTSSALTLVVDL
                     VFTVVFLAVMAYYSLFLTGIVLASFPFYIAISAIATPIFRRRLEEKFNRGAENQAFLV
                     ESVTGIETLKAMAVEPQMQRRWEEQLAAYVRASFRVLSLGNWASQGVQLISKLVTAGV
                     LYFGAKAVIDGDLTVGELVAFNMLAGRVAQPVLRLAQLWQDFHQTRISIARLGDILNT
                     HPEPTYTPGRAALPAIRGAVTFEHVTFRYRIDGAEVLHDVSLPIPAGQVVGIVGPSGS
                     GKSTLAKLVQRFYVPESGRVLVDGVDLAAVDVTWLRRQVGVVLQENVLFNRTVRDNIA
                     LADPSMPVERVIRAAKLAGAHEFILELPQGYDTMVGERGASLSGGQRQRIAIARALVT
                     DPRILIFDEATSALDYESERVIHENMRGISQGRTVLIIAHRLSSVRRADRIVTIERGR
                     LVEDGNHDELLRTGGRYANLHRLQAGVHEAG"
BASE COUNT          400 a          978 c          863 g          498 t
ORIGIN      
        1 cccgcgccct cggcttgcca cgccgcccga ttgaagtgat cgaggccgta gacgccggcg
       61 ccgaaggctt gatgcagggc cgcgaccgtg gcgatgagcg cgcgcaggcg ctcgtcgcgc
      121 gcatcgccgc gatgcgaccg gttggtcgcc tgcgggtcga tcgcccggaa gaaatgcggg
      181 atcacgatca ggatgcgcat ggctgctccg agtgtggggg gtccgcagtc gctccggtga
      241 tctacgaata cctggtcttt ccgcaatatt aacgttcgat ggcgcagaga taccaagtct
      301 atattgttgc gaagatatca cataatcaat aatggtcagg attatatcgg tggaaggccc
      361 tgttaaatag cgacaatcgt cctctgaatg cgtcggacta ggccgagttt ttctgtgtct
      421 gtccgttttg tcttgtgcag acactacgcg atatcatagg ttcgatgcaa ccggaggctt
      481 ccttgttcat tccctttcat tcaaccctgc ggcgacgatg ccgctcgagc tagccccggc
      541 cgaggcggcc gcggacggtg ccgcggcgct ggtgctgctg ctgcgttttc acggcgtcgc
      601 cgccgatccc gcgcagatcc gccaccgctt cggcggcgcc gcgatcggcc tgcccgagat
      661 ggtgcgctgt gcgcgggagc tgaagctgcg cgcccgcgcc gtccattccg attgggagcg
      721 cctcgccaag acgccgcttc ccgcgatcgc cgagcggcgc gacggcggct ttttgatcct
      781 cgccaagctt tccaatgagg gcgtgctggt gcaggacccg ggcgccggcc ggccccaggt
      841 gctgccgcgc gccgagttcg agcagcaatg gagcgggcgc ctcttgctga tgacgcgccg
      901 cgcgagcctc tccgacctgg cgcgccgctt cgacgtcact tggttccttc aggcgatgca
      961 caaataccgc cgcctcctcg ccgaggtgct ggtcgcgtcc ttcttcctcc agctcttcgc
     1021 cttggtgacg ccgctcttct tccaggtggt gatcgacaag gtgctggtgc atcgcggcct
     1081 caccaccctc gacgtgctca tcgtcggcct cgtcaccgtc tcgctcttcg agagcgtgct
     1141 gggcgcgctg cgcacctatg tcttctcgca caccacgaac cgcatcgacg tcgagctcgg
     1201 cgcgcggctc ttccgccatc tcctggcgct gccgatcgcc tacttcaacg cgcgccgcgc
     1261 cggcgacagc gtcgcgcgcg tgcgcgagct cgagaacatc cgcaacttcc tcaccagctc
     1321 ggcgctgacc ctcgtcgtcg acctcgtctt caccgtcgtg ttcctggcgg tgatggcgta
     1381 ttactcgcta ttcctgaccg gcatcgtgct cgcgtccttc ccgttctata tcgcgatctc
     1441 cgccatcgcc acgccgatct tccgccggcg cctcgaggag aagttcaacc gcggcgccga
     1501 gaaccaggcc ttcctggtgg agagcgtcac cggcatcgag accttgaagg cgatggcggt
     1561 cgagccgcag atgcagcgcc gctgggagga gcagctcgcc gcctatgtgc gcgccagctt
     1621 ccgcgtgctc tcgctcggca actgggcgag ccagggcgtg cagctcatca gcaagctcgt
     1681 caccgccggc gtcctctatt tcggcgccaa ggcggtgatc gacggcgacc tcacggtggg
     1741 cgagctcgtc gccttcaaca tgctggccgg ccgcgtcgcg cagccggtgc tgcgcctggc
     1801 ccagctctgg caggatttcc accagacgcg catctcgatc gcgcgtctcg gcgacatcct
     1861 caacacccat cccgagccca cttacacgcc gggccgcgcc gcgctgccgg cgatccgcgg
     1921 cgccgtcacc ttcgagcacg tcaccttccg ctatcgcatc gacggcgccg aggtgctgca
     1981 tgatgtcagc ctccccatcc ccgcggggca ggtggtcggc atcgtcggcc cctcgggctc
     2041 cggcaagtcg acgctggcga agctggtgca gcgcttctac gtgcccgaaa gcggccgcgt
     2101 gctcgtcgac ggcgtcgatc tcgcggcggt cgatgtcacc tggctgcgcc gccaggtcgg
     2161 cgtcgtgctg caggagaatg tcctcttcaa ccgcacggtc cgcgacaaca tcgcgctcgc
     2221 cgatccgagc atgccggtcg agcgcgtcat ccgcgcggcg aagctcgccg gcgcccatga
     2281 gttcatcctc gagctgccgc aaggctacga caccatggtg ggcgagcgcg gcgccagcct
     2341 ctcgggcggc cagcgccagc gcatcgccat cgcccgcgcc ctcgtcaccg atccgcgcat
     2401 tctcatcttc gacgaggcga cgagcgcgct cgactacgag agcgagcgcg tcatccacga
     2461 gaacatgcgc ggcatttcgc aagggcgcac ggtgctgatc atcgcccatc gcctgtcgag
     2521 cgtgcgccgc gccgaccgca tcgtcaccat cgagcgcggc cgcctcgtcg aggacggcaa
     2581 ccatgacgag ctgctgcgca ccggcggccg ctatgcgaac ctccatcgcc tccaggcggg
     2641 cgtccatgaa gccggctgag caaagtgcag cgggcgcgcg catcctgccc tggccgcgcc
     2701 ttctggcgcg cagcggcggc gaatcggaat tcctgccgg
//
LOCUS       DASYNS010000026         7360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3284023, whole genome shotgun sequence.
ACCESSION   DASYNS010000026 DASYNS010000000
VERSION     DASYNS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3284023"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VGP50_00825"
     CDS             complement(<1..183)
                     /locus_tag="VGP50_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025936.1"
                     /translation="MIREQHAQADTVELGVGDQVASRYPPLAALMGRNALEPQAKVAR
                     DDLTRQTALDAQASQTP"
     gene            315..2456
                     /locus_tag="VGP50_00830"
     CDS             315..2456
                     /locus_tag="VGP50_00830"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEV8025937.1"
                     /translation="MDGLIKKLGELSGSGPNRIALSTAMRGTIATALPLLLLPQLGLG
                     HLTYPAVLGALATSMVDVGGPYRARLLAMLAQALGGPCLLLLGSAAVEHWWLAALVMA
                     AIGVASGLIRALGPGGAALGVNSAVAFLVGLQVGEIGSPLEPWVVGYGAGGLWTILVA
                     LVFWQLRPYRRLEQEVASAWEAVASLVATIGAEEGSVVARRRREQRIAAAHAAVRNAV
                     ERARAALGETRAGTAGPGTTMAQLVVLLTAAARIAAAAVTLSEVAAPPGSERLAAEAE
                     LGGACRAVARVLLAGKGELPLAELRRRLDAAHSGRGEPQGHADLLAWAQAMRHLDNAE
                     EALRVLFGVRRRLAELWRLPFADRPPRGAALAALRAQLTPRSAIFRHAMRVALVTGSE
                     SMALAYFHLPHGIWLPLTSLVVMQPDYGGTIARAVARGAGTIAGAVLAGILLTAAHSK
                     AAYDAALGLLLFATFLLIRRRYGYGIMFLTPIIILLIGASSADPWIDLAERVAYTVVG
                     AVLALAAGYLLWPRWEHEQLRDRLADAILADTAYLGAMLSAFAQPAAEAATLPALRRQ
                     AEIAVANADAGFQRMLGEPAHPARLVAAGFSLLVYLHRLCRHTIALAAQLGAASPLPP
                     EPLAALRRRMTAALEDTALALKEERAPAPRPDFDTPLEELMAHVGGAAEGATGALLGR
                     LVSDATGVLGATGAAVRTARGAQEGSTAQAG"
     gene            complement(2565..3077)
                     /locus_tag="VGP50_00835"
     CDS             complement(2565..3077)
                     /locus_tag="VGP50_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV8025938.1"
                     /translation="MQQRHNKNTVTTDLAPEAVRDLSGALNALLADMFALYLKTKNFH
                     WHVAGPHFRDYHLMLDEHGEQIFATTDDIAERVRKIGGTTLRSIGHIAKLQRVEDNDA
                     EYVTALDMLAELRDDNRRLVSRLRETHDLCDEHGDVASASLLENWIDEAERRAWFLFE
                     ATRTDESASR"
     gene            complement(3377..4327)
                     /locus_tag="VGP50_00840"
     CDS             complement(3377..4327)
                     /locus_tag="VGP50_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025939.1"
                     /translation="MRRLPRAIPFVALALTLWGCSINDLLLEPKEAQETQAADPVDPR
                     RNGDLPEEIAVTNAEGRRLVGWLFTSPGDRGTVLVAGGNGMGIAHTYAYNRFLLGHGF
                     RVLVFSYQGFDDNEGKADIASLPGDAEAFHAAMRGRFPGEPVAFLGESIGAIAGFCDS
                     AAGDFSALALEGLIDPKSVAGTIIDSHFPSPLSDVMSFAVGRMSVFYSAAVPDALSAA
                     GCSTRLRGAEVLFLHQRRDPVASFATVEHLAQLRPRHSTIVELPAPPSHAHLSLANNA
                     AGQAQVVAFLRDNLAKASVAARTDAARLSASSPRPPAPVE"
     gene            complement(4685..5977)
                     /locus_tag="VGP50_00845"
     CDS             complement(4685..5977)
                     /locus_tag="VGP50_00845"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEV8025940.1"
                     /translation="MSDLPRGPGFETLAVHAGAAPDPATGARSTPIYQTTAYVFDDVD
                     HAASLFNLQTYGYIYSRLTNPTVSVLEERLATLEGGRGAICVASGHAAQLLAFFSFME
                     PGDQFVASNKLYGGSITQFGRSFKKFGWNAVLVDPDDPENFRRALTPRSKAIFVESLS
                     NPTGVVADLAAIARIAHDAGVLFIVDNTMASPWLCRPIEWGADLVIHSTTKFLSGHGN
                     AMGGVVIDSGKFDWAQNDKFKSLTEPEPAYHGLRFYETFGDLALTVHGHAVGLRDLGA
                     TMAPLNAFLTITGIETLALRMARHVENAQAVAEHLARHPAISWVAYAGLPHSRYHALA
                     RKYLPKGAGAVFTIGLKGGYDAGVKLVESVELFSHLANVGDTRSLIIHPASTTHRQLS
                     PEQQIAAGAGPDVVRLSIGIESIADIIADLDQGLSANA"
     gene            complement(5979..6458)
                     /locus_tag="VGP50_00850"
     CDS             complement(5979..6458)
                     /locus_tag="VGP50_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV8025941.1"
                     /translation="MNHDGYTDAYLREILKSVRLIAMVGASAHWNRPSYFAMKYLQDK
                     GYRVIPVNPQALGQEILGEKVYGDLSEVPEKIDMVDIFRRSEAAPALVDQAIAKGAKV
                     VWMQLGVRDDAAAAKAEAAGLKVVMNRCPKIEYSRLNGELAWSGVNTGIISSKRRKI"
     gene            complement(6455..6715)
                     /locus_tag="VGP50_00855"
     CDS             complement(6455..6715)
                     /locus_tag="VGP50_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025942.1"
                     /translation="MADCPRFGKFFRGCLFVGRYDSIAPTADVIAAMRSIVDSGQVED
                     EIADVLGALSSSIYVRDVCIRCGTTVERAKGVAASPPPLAAS"
     gene            complement(6719..>7360)
                     /locus_tag="VGP50_00860"
     CDS             complement(6719..>7360)
                     /locus_tag="VGP50_00860"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV8025943.1"
                     /translation="PAIKVVVIGGAGPGFCAGHDLREMRANPERQHYETLFAQCSRLM
                     TALVRLPKPVIASVHGIATAAGCQLVASCDLAVASSAARFGVNGIDVGLFCSTPMVAL
                     TRAVGRKPAMEMLLTGDLVDAVHARTIGLVNRVTAPAQLEDETRALARQIASKLPLAV
                     ALGKEAFYRQAELGLDAAYTYTSEVMTRNMLAPETEEGIDAFLEKRPPRWDRD"
BASE COUNT         1119 a         2440 c         2634 g         1167 t
ORIGIN      
        1 aggcgtttgc gacgcttgcg catcgagggc ggtttgacgc gtaagatcgt cgcgggccac
       61 cttggcctgc ggctcgagcg cgttgcggcc catgagcgcg gcaaggggcg gatagcggct
      121 cgcgacctga tcgcctacgc cgagttctac ggtgtccgct tgagcgtgtt gttcgcggat
      181 cattgagacg cgggccgggc tggaacagat ccgtgacccc caccccaacc cttcccctca
      241 gggggggagg gggatgattg ttgctctggc gcgaacgacg aggcggccgg cgggcggcta
      301 tgatgcgtgg cgccatggat ggcctcatca agaaactcgg cgagctgtcg ggcagcgggc
      361 ccaatcgcat tgcgctgtcg acagcgatgc ggggcacgat tgccaccgcg ctcccgctgc
      421 tgttgctgcc gcagctcggc ctcggccatc tcacctatcc cgccgtgctc ggcgcgctcg
      481 ccacgtcgat ggtcgatgtc ggcgggccct atcgcgcgcg gctcctcgcc atgctcgccc
      541 aggcgctggg cggaccctgc ctgctgctcc tggggagcgc ggcggtcgag cattggtggc
      601 tcgccgccct tgtcatggcg gcgatcggcg tcgcctccgg ccttatccgt gcgctcgggc
      661 ccggcggtgc cgcgctcggc gtcaacagcg ccgtcgcctt ccttgtcggc cttcaggtcg
      721 gcgagatcgg cagcccgctc gaaccctggg tcgtgggcta tggcgcgggc gggctctgga
      781 ccatcctcgt cgccctcgtc ttctggcagc tccgccccta ccgccgcctc gagcaggagg
      841 tggcgagcgc gtgggaggcc gttgcgtcgc tggtcgcgac catcggcgcg gaagagggga
      901 gcgtcgtcgc tcggcggcgc cgcgagcaac gcatcgccgc ggctcatgcc gccgtgcgca
      961 atgcggtgga gcgggcgcgc gcggcgctgg gcgagacgcg cgcgggcacg gcgggcccgg
     1021 gcacgacgat ggcccagctc gtcgtgctgc tcaccgccgc ggcgcgcatc gccgccgcgg
     1081 ccgtgacctt gagcgaagtc gcggcaccgc cggggagcga gcggctcgcc gcggaggccg
     1141 agctcggagg cgcctgccgg gccgtggcgc gcgtccttct cgccggcaag ggagaattgc
     1201 cgctcgccga gctccgccgc cggctcgatg cggcccatag cgggcgcggc gagccccaag
     1261 gccacgcgga tctcctggcc tgggcgcaag cgatgcgcca tctcgacaat gcggaggagg
     1321 cgctgcgcgt gctgttcggc gtgcggcggc ggctcgcgga gctgtggcgg ctgccctttg
     1381 ccgatcgccc gccccgcggc gcggctctcg ccgcactccg cgcgcaactg acgccgcgct
     1441 cggctatctt ccgccatgcc atgcgcgtcg cgctcgtcac cgggagcgaa tccatggcgc
     1501 tcgcctattt ccacttgccg cacggcatct ggctgccgct cacatcgctg gtcgtcatgc
     1561 agcccgatta tggcggcacg atcgcgcgcg cggtcgcgcg cggtgctggg accatcgccg
     1621 gcgcggtgct ggcgggaatc ctgctgacgg cggcgcacag caaggcggcc tatgatgcgg
     1681 cgctcggcct cctgctcttc gcgacgttcc tgctgatccg ccgccgctac ggctatggga
     1741 tcatgttttt gacgccgatc atcatcctgc tgatcggcgc cagcagcgcc gatccgtgga
     1801 tcgatctcgc cgagcgcgtg gcttatacgg tggtgggcgc ggtgctggcg ctcgccgcgg
     1861 gatatctgct ctggccgcgc tgggagcatg agcagctgcg cgaccggctg gccgacgcga
     1921 tccttgccga cacggcttat ctcggagcaa tgctgtcggc ctttgcccaa cccgccgcgg
     1981 aagctgcgac gcttcccgct ctccggcgcc aggcggagat cgccgtggcc aatgccgatg
     2041 ccgggtttca gcgcatgctc ggcgagccgg cgcatcccgc gcggctcgtt gccgccggat
     2101 tctcactcct ggtctacctg catcggctgt gccgccacac gatcgcactc gcggcccagc
     2161 tcggtgcggc gtcgccgctg ccaccagagc cgctcgccgc cctccggcgc cgcatgacgg
     2221 cggcgctcga ggacacggcc cttgcgctga aggaggagcg agcgccggcg ccacggcccg
     2281 atttcgatac gccactggag gagctcatgg cacatgtcgg cggcgcggcc gaaggtgcca
     2341 ccggcgcgct gctcgggcgg ctggtgagcg acgcgacggg cgtgctcggc gccacgggcg
     2401 ccgccgtcag aacggcgcgc ggtgctcaag aagggagcac ggctcaggcc ggatgacgcg
     2461 acgccggctg cggttccgca atccggcagc gcgccgcgcc gtcgccgcgc gctgccgccg
     2521 gagacacgaa ctcgacgctc agcgaaccgg cgacggcggt gagatcaccg cgaggccgat
     2581 tcgtcggttc gggtcgcctc gaagaggaac caggcccgcc gctcggcttc gtcgatccaa
     2641 ttctcgagga ggctggcgct ggcgacgtcg ccatgctcgt cgcagaggtc atgcgtctcg
     2701 cgcaggcgcg agaccagccg gcgattgtcg tcgcggagct cggccagcat gtcgagggcc
     2761 gtcacatatt cggcatcatt gtcctcgacg cgctgaagct tggcgatatg gccgatcgag
     2821 cggagcgtcg tcccgccgat cttgcgaacg cgctcggcga tgtcgtcggt cgtcgcgaag
     2881 atctgctcgc catgctcgtc gagcatcagg tggtagtcgc ggaaatgcgg ccccgccacg
     2941 tgccagtgga aattcttggt cttcagatag agcgcgaaca tgtcggcgag gagcgcgttg
     3001 agcgcccccg aaagatcgcg cacggcctcg ggagcaaggt ccgtcgtgac ggtattcttg
     3061 ttgtgacgct gctgcatggc ttctgcctcc gtggaatggt tccgctcgac gaacgtcacg
     3121 cgcgtccgcg cggcgccgcg tgcccggctt acataggcga acgccgacgg acccgaaagg
     3181 gcacgaatcc ttgagcgaac gtagccgtca aggcatctga aggtatagtc gccgaccccg
     3241 ccatgcccgc gcaggaatcc cggcgatcat tcccaatcct ccacgcgcac cgccggtcga
     3301 ttgcgcgccc agcgcaagac cggcgagttc gggcgcagga taccgttcat cggcagaccc
     3361 gcaatgcctc gccgcgctac tcgacaggcg ccggcggccg gggggaggag gcggagaggc
     3421 gcgccgcatc cgtgcgcgcg gcgacgctcg ctttggcgag gttgtcgcga aggaaggcga
     3481 cgacctgcgc ctgcccggca gcgttgttcg cgagcgagag atgggcgtgg ctcggcggcg
     3541 ccggcaattc gacgatcgtg ctgtggcgcg gccgcagctg cgccagatgc tccaccgtcg
     3601 cgaaggaagc gacgggatcg cggcgctgat ggaggaagag cacctcggcg ccgcgcaaac
     3661 gggtgctgca gccagcggca ctaagcgcgt cgggcacggc cgccgaatag aagacggaca
     3721 tccgccccac ggcgaaggac atgacgtccg agagcggcga ggggaagtgg ctgtcgatga
     3781 tggtgccggc gaccgatttc ggatcgatca ggccttcgag cgcgagggcg gagaaatcgc
     3841 cggcggcgct gtcgcagaag ccggcgatcg cgccgatgga ttcgccgagg aaagcgacgg
     3901 gctcgccggg gaagcggccg cgcatcgcgg cgtgaaacgc ttccgcgtcg ccgggcagcg
     3961 acgcgatgtc cgccttgccc tcgttgtcgt cgaagccctg ataggagaag accaggacgc
     4021 ggaagccatg cccgagcagg aagcggttat aggcgtaggt gtgggcaatg cccatgccgt
     4081 tgccgccggc caccagcaca gttccgcggt cgccgggtga cgtgaagagc cagccgacga
     4141 gccggcgccc ctccgcgttg gtgacggcga tctcctccgg cagatcgccg ttgcgacggg
     4201 gatcgacggg atcggcggcc tgcgtctcct gcgcttcctt gggctcgagc agcaggtcgt
     4261 tgatgctgca gccccaaagg gtcagagcga gcgcaacgaa aggtatggcg cgagggagcc
     4321 ggcgcatgcg ccgaggattt agcaccgcgc cgctaaaata cccgttaaca caggatttta
     4381 cggcattttt cgggaactgc ggcggagcga tcgccggcat ggccgggatc gctttggcac
     4441 attcctccaa cactctctac gaacgctcgt cgtcttactt cggcacgaag gaatggcccc
     4501 gtgccgaaaa tttcgacctc agcgttagcg cgacgcgaag cgtcgtcgct ggcgggcgtc
     4561 agcggttgct cctgcaaccg cgggagcccg cacagagcgc gatgtatcgg ctggcgggta
     4621 ccccgctcgg tgcggctctc gcgtccctct ttcgcgcgga cgctgacgcc gccgcggacg
     4681 aacgctatgc gttcgccgag aggccttggt ccaggtcagc gataatatcg gcgatgctct
     4741 cgatcccgat cgagaggcgg acgacgtcgg gaccggcgcc ggcggcgatc tgctgctcgg
     4801 gcgagagctg gcgatgcgtg gtcgaggccg gatggatgat cagcgagcgc gtgtcgccga
     4861 cattggcgag atgggagaag agctcgacgc tctccaccag cttgacgccc gcgtcatagc
     4921 cgcccttgag cccgatggtg aagacggcac ccgcgccctt cggcaggtat ttgcgcgcca
     4981 gcgcgtgata acggctgtgc ggcagcccgg cataagcgac ccaggagatg gcggggtgcc
     5041 gtgcgagatg ctcggccacg gcttgcgcat tctcgacatg gcgcgccatg cgcaaggcca
     5101 gcgtctcgat gccggtgatg gtgaggaagg cgttgagcgg cgccatggta gcgccgaggt
     5161 cgcgcaagcc cacggcatgg ccgtgcacgg tgagtgccag gtcgccgaag gtctcgtaga
     5221 agcgcaggcc atgataggcg ggctcgggct cggtcaggga tttgaacttg tcgttctgcg
     5281 cccagtcgaa cttgccgctg tcgatgacga cgccacccat ggcgttgccg tggcccgaga
     5341 ggaacttcgt cgtcgaatgg atgacgagat cggcgcccca ctcgatgggg cggcagagcc
     5401 aaggcgacgc catcgtgttg tcgacgatga agagcacgcc cgcatcatgc gcgatccggg
     5461 cgatggcggc gaggtcggcg acgacgcccg tcgggttcga caggctctcg acgaagatcg
     5521 ccttcgagcg cggcgtcagc gcgcggcgga aattctcggg atcgtcgggg tcgaccagca
     5581 cggcgttcca gccgaacttc ttgaagctgc ggccgaactg cgtgatcgac ccgccataga
     5641 gcttgttcga ggcgacgaac tggtctcccg gttccatgaa ggagaagaag gccagcagct
     5701 gcgcggcatg gcccgaggcg acgcagatgg cgccgcggcc gccttcgagc gtcgcgagcc
     5761 gctcttcgag caccgagacc gtcgggttgg tgaggcgcga atagatgtag ccataggtct
     5821 gcagattgaa cagtgacgcc gcgtggtcga cgtcgtcgaa cacgtaggcc gtcgtctggt
     5881 agatcggcgt gctgcgcgcg ccggtcgcgg gatcgggcgc ggcgccggca tgcacggcca
     5941 gcgtctcgaa gccggggccg cgggggaggt cgctcatgtc agatcttcct gcgcttcgac
     6001 gagatgatgc cggtgttgac gccgctccag gcgagctcgc cgttgaggcg cgaatactcg
     6061 atcttcgggc agcggttcat caccaccttg aggccggccg cctcggcctt ggccgcggcc
     6121 gcgtcgtcgc gtacgccgag ctgcatccac acgacctttg cgcctttggc gatcgcctgg
     6181 tcgacgaggg caggggccgc ctccgagcgg cgaaagatgt cgaccatgtc gatcttctcc
     6241 ggcacctcgg acaagtcgcc atagactttc tcgccgagga tctcctggcc gagcgcctga
     6301 ggattgacgg gaatgacgcg atagcccttg tcctggagat acttcatcgc gaagtaggag
     6361 ggccggttcc aatgcgcgct ggcgccgacc atggcgatga ggcgcacgga tttgaggatc
     6421 tcgcgcagat aggcatcggt gtagccgtcg tggttcatga tgcggccaag ggcggcggcg
     6481 acgcggcgac tcccttcgcg cgttccaccg tcgtgccgca gcgtatgcac acgtcgcgga
     6541 cgtagatcga cgacgagagc gcccccagca cgtcggcgat ctcgtcctcg acctgcccgg
     6601 agtccacgat cgaccgcatg gccgcgatga cgtcggcggt gggcgcgatg ctgtcatacc
     6661 gccccacgaa caagcagccg cggaagaact ttccgaaacg cgggcagtcc gccatcgctc
     6721 agtcccgatc ccaacgcggc gggcgcttct cgaggaaggc atcgatccct tcctcggtct
     6781 cgggcgccag catgttccgg gtcatgacct ccgacgtgta ggtataggcg gcgtcgaggc
     6841 cgagctcggc ctggcgatag aaagcttcct tgccgagagc gacggcgagc gggagcttcg
     6901 acgcgatctg ccgcgccagc gctcgcgtct cgtcctcgag ctgcgcgggc gcggtcacgc
     6961 ggttgacgag cccgatggtg cgcgcatgga cggcgtcgac gagatcgccg gtgagcagca
     7021 tctccattgc cggcttgcgg ccgaccgcgc gtgtcagcgc caccatcggc gtcgagcaga
     7081 agagcccgac atcgatcccg ttgacgccga agcgcgccgc gctcgaggcg acggcgagat
     7141 cgcaggaggc gacgagctgg caacccgccg ccgtggcgat gccgtgaacc gaggcgatga
     7201 ccggcttcgg caggcggacg agcgcggtca tgagccgcga gcattgcgcg aacagcgtct
     7261 cgtaatgctg gcgctccgga ttggcgcgca tctcccggag atcatggccg gcgcagaaac
     7321 cggggccggc gccgccgatc accacgacct tgatcgcggg
//
LOCUS       DASYNS010000027         3817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_476086, whole genome shotgun sequence.
ACCESSION   DASYNS010000027 DASYNS010000000
VERSION     DASYNS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476086"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..3657
                     /locus_tag="VGP50_00865"
     CDS             172..3657
                     /locus_tag="VGP50_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[ACP]--phospholipid O-acyltransferase"
                     /protein_id="HEV8025944.1"
                     /translation="MAETQLHLLKSRRLLPLFVTQFLGAVNDNLFKTALVTLVTFLPD
                     YSVGSDAEREIIVTIATAIFIFPYFPLSAMGGQLADKFEKAALIRWVKLWEIGVMVLA
                     AIGLLTLTHRAFIYFELAVLFLLGVQATFFGPVKYGILPNLLAREDLMGGNALIEAGT
                     FLAILVGIIAGGLLPAFTIGAQWLGPEAVSGALLACAVAGWAASWLIPRVGRAAPELR
                     ISPNIWAETFNIIRFAWARRDLKLAIIAISWFWLVGAVFLSQFPTYTQGTLGADEQVQ
                     TLLLAMFSIGIGAGSVVCGRLLRGEVSARLAPLGAVGMALFSADLFFASDQVVGGNGS
                     ELLGVFAFLAHPANWRVLFDLFMIALCGGLFTVPLYALLQERSEESHRSRVVAANNII
                     NALFIVVSSIITAVLLYFRVTVSQVFLIVGLLNAVAAIVVMRLVPGVILKTMLAALFR
                     LVYRVEVKGAENYRKAGDRVVIIANHLSFLDGVLLAAFLPGRPAFAIDTAQAAVWWRK
                     PFLELFDAVPLDPTRPFATKVLIRAVEEGRHCVIFPEGRITVTGALMKVYEGPGMIAD
                     KAKATILPVRLEGPQYTRFSRLRGKVRLRWFPKVTITLLEPQEIALSPEVKGRARRRE
                     IGRRLYEVMSAMMFASADTRRTLFQALIDARHLHGGTRPILEDIERKPMGYDRLLAAS
                     FALGRRLAERSAPGEMVAVLLPNAAGAVATFFALHAYGRVPALLNYSTGAANMLAACK
                     LADIATIITSRRFIERAKLKDALATIADRRRVLYLEDLRKDISLGESLRALLTARFAH
                     LLQRRATAAPGDAAVVLFTSGSEGQPKGVVLSHENILANCHQLAARVDFSPSDILFNA
                     LPIFHSFGLTGGMLLPLVSGVRLFLYPSPLHYRIVPEACYDTNATILFGTDTFLTGYA
                     RAANAYDFYSLRYVFAGAEKVHDETRRQWTEKFGLRILEGYGATETSPALATNTPMHF
                     RAGTVGRLLPGISYRLEPVEGVSEGGRLHVAGPNVMKGYLSATKPGAIDAPPGGWYDT
                     GDIVAIDGEGYVRILGRAKRFAKIAGEMVSLGAVEALAASLWPGFRHAALALPDARKG
                     EQVVLLTDRPAATPEALLAQARAQGIAEVMVPRVVVPVPAVPLLGTGKLDYATARALA
                     EEKLGARQGATGTTG"
BASE COUNT          544 a         1416 c         1179 g          678 t
ORIGIN      
        1 ggacgacccc gagctcggcg ccgcgctggt gatgggcatc atcatccagc ctgcgacctt
       61 caagacctat ggccggctgg ccggaccgat gctgccgctc gccagccgcc tcaccgccgc
      121 ttgctggagc atcctccagg cttagcccct ttcaaaagac gagatcacgc catggccgag
      181 acccagcttc atctcctcaa gagccgccgc ctgctgccgc tcttcgtcac ccagttcctg
      241 ggcgcggtga acgacaacct gttcaagacc gcgctggtga cgctcgtcac cttcctcccc
      301 gactacagcg tcggcagcga cgccgagcgc gagatcatcg tcaccatcgc cacggccatc
      361 ttcatcttcc cctatttccc gctctcggcc atgggcgggc agctcgccga caaattcgag
      421 aaggcagcgc tcatccgctg ggtcaagctc tgggagatcg gcgtcatggt cctggcggcg
      481 atcggtctgc tgacgctcac gcaccgcgct ttcatctatt tcgagctcgc cgtgctgttc
      541 ctgctgggcg tccaggcgac gttctttggt cccgtcaaat acggcatcct gcccaacctg
      601 ctggcgcgcg aggacctgat gggcggcaac gcactcatcg aggcgggcac cttcctcgcc
      661 atcctcgtcg gcatcatcgc cggcggcttg ctgcccgcct tcaccatcgg cgcgcagtgg
      721 ctcgggccgg aggccgtctc gggcgcgctg ctggcctgtg ccgtcgccgg ctgggcggcg
      781 agctggctca ttccgcgggt cggtcgcgcg gcgccggagc tgcgcatcag tccgaacatc
      841 tgggcggaga ccttcaacat catccgcttc gcctgggcgc ggcgcgatct caagctcgcg
      901 atcatcgcca tctcctggtt ctggctggtg ggcgccgtct tcctctcgca attccctacc
      961 tatacccagg gcacgctcgg cgccgacgag caggtccaaa ccctcctcct cgccatgttc
     1021 tcgatcggca tcggcgcggg ctcggtggtc tgtggcaggc tgctgcgcgg cgaggtgagc
     1081 gccaggctgg caccactggg cgccgtcggc atggcgctct tctctgccga tctcttcttc
     1141 gcgagcgatc aggtcgtcgg cggcaacggc agcgaattgc tcggcgtctt tgcctttctc
     1201 gcccaccccg ccaattggcg cgtgctcttc gacctcttca tgatcgcgct gtgcggcggt
     1261 ctcttcaccg tgccgcttta cgcgctcctc caggagcgca gcgaggagag ccatcgctcc
     1321 cgcgtcgtcg ccgccaacaa catcatcaac gcgctgttca tcgtcgtctc cagcatcatc
     1381 accgccgtgc tcctctattt cagggtgacg gtgtcgcagg tcttcctcat cgtcggcctc
     1441 ctgaacgccg tggccgccat cgtggtgatg cgcctcgtgc cgggcgtcat tctgaagacg
     1501 atgctcgccg cgctcttccg gctcgtctat cgcgtcgagg tgaagggcgc cgagaattac
     1561 cgcaaagccg gcgaccgcgt ggtcatcatc gccaatcatc tctcctttct cgatggcgtg
     1621 ctgctcgccg ccttcctgcc gggccgcccc gctttcgcca tcgacacggc gcaggccgcg
     1681 gtgtggtggc ggaaaccgtt cctcgagctc ttcgacgccg taccgctcga tccgacgcgt
     1741 cccttcgcca ccaaggtgct gatccgcgcc gtcgaggagg gccgccattg cgtcatcttc
     1801 cccgagggcc gcatcaccgt caccggcgcc ttgatgaagg tctatgaggg ccccggcatg
     1861 atcgccgaca aggccaaggc gacgatcctg ccggtgcggc tcgaaggccc gcaatacacg
     1921 cgcttctcgc ggctgcgcgg caaagtgcgg ctgcgctggt tccccaaggt gacgatcacg
     1981 ctgctcgagc cgcaggagat cgccctctcg cccgaggtca aaggacgcgc gcgccggcgc
     2041 gagatcggcc gccgcctcta cgaggttatg tcggcgatga tgttcgccag cgccgacacc
     2101 aggcgcacgc tcttccaggc gctgatcgat gcgcggcatc tccatggcgg aacgcggccg
     2161 atcctcgagg acatcgagcg caagcccatg ggctatgacc ggctgctggc ggccagcttc
     2221 gcgctgggcc ggcgcctcgc cgagcgcagc gcgccgggcg aaatggtcgc cgtgctcctg
     2281 cccaatgcgg ccggcgccgt cgccactttc ttcgccctcc atgcctatgg ccgggtgccg
     2341 gcgctcctca actactcgac gggcgcggcc aacatgctgg ccgcctgcaa gctcgccgac
     2401 atcgccacca tcatcacgtc gcggcgcttc atcgagcgcg ccaagctcaa ggatgcgctg
     2461 gcgaccatcg ccgatcgccg gcgcgtcctc tatctcgaag acttgcgcaa ggacatcagc
     2521 ctgggcgaga gcctccgcgc gctgctgacg gcccgctttg cccatctgct gcagcgccgc
     2581 gccacggccg cgcccggcga cgccgcggtc gtgctcttca cctcgggctc ggaaggccag
     2641 cccaaaggcg tcgtgctgag ccacgagaac attctcgcca attgccacca gctcgccgcg
     2701 cgcgtcgatt tctcgcccag cgacatcctc ttcaatgcgc tgcccatctt ccactccttc
     2761 gggctcaccg gcggcatgct gctgccgctc gtctcgggcg tgcgcctctt cctctaccct
     2821 tcgccgctcc attaccggat cgtgcccgag gcctgctacg acaccaacgc caccatcctt
     2881 ttcggcacgg acaccttcct caccggctac gcgcgcgccg ccaacgcgta cgacttctac
     2941 agcctgcgct atgtcttcgc cggggccgag aaggtgcacg acgagacgcg ccggcaatgg
     3001 accgagaaat tcggcctgcg catcctcgaa ggctatggcg cgacggagac ctcgcccgcg
     3061 ctcgccacca acacgccgat gcatttccgg gcgggcacgg tgggccgtct gctgcccggc
     3121 atcagctatc gcctcgagcc cgtcgagggc gtatccgagg gcgggcgcct tcatgtcgcc
     3181 ggccccaatg tcatgaaggg ctatctcagc gcgaccaagc ccggcgccat cgatgcgccg
     3241 ccgggcggct ggtacgacac gggcgacatc gtcgcgatcg atggcgaagg ctatgtccgc
     3301 atcctcggcc gcgccaagcg ctttgccaag atcgccggcg agatggtgtc gctgggtgcc
     3361 gtcgaggcgc tggccgcctc gctctggccc gggttccgcc atgccgcgct cgcactcccc
     3421 gacgcgcgca agggcgagca ggtcgtgctg ctgaccgatc gcccggccgc cacacccgaa
     3481 gcgctcctgg cgcaggcgcg cgcgcagggc attgccgagg tgatggtgcc gcgggtggtc
     3541 gtaccggtcc ccgccgtgcc gctgctcggc acgggcaagc tcgattacgc gaccgcgcgc
     3601 gctctcgcgg aagagaaact cggcgcgcgg caaggcgcga ccggcacgac gggataatcc
     3661 ctcccggtct ggtgttcggc gccggcatgg gctacccatg tcgctccggc cgccaggccg
     3721 agggaggatt cgatgcgcgc catgaaggtg atttcgatcg gcatctgcgt cagcgccgcc
     3781 gcgctcatcg ccgcacgcgc ggccgacaac acgccgc
//
LOCUS       DASYNS010000028         7116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4376996, whole genome shotgun sequence.
ACCESSION   DASYNS010000028 DASYNS010000000
VERSION     DASYNS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4376996"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1428)
                     /locus_tag="VGP50_00870"
     CDS             complement(190..1428)
                     /locus_tag="VGP50_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8025945.1"
                     /translation="MMEWMGRLTAAERRTFVAAFGGWAMDAMDFMVFSFVIPTLLVLW
                     KISTGEAGVLATVTLVVSALGGWIAGMLADRFGRVRILQLTILWFAVFTFLNGFADSF
                     EMLFTTRALQGLGFGGEWAVGSVLVGEMIAAEHRGRAVGVMQSAWAIGWGIAAILATL
                     FFKLLPETIAWRALFWVGFAPALLVYYVRRFVPEPQVFNETRRRAAVGGTLNSFEIFA
                     PPLLYTTVFASLLATGAQGGYYAVTIWLPTFLRTVRHLTVLGSGAYLAVIIVGSFAGY
                     LVSAWITDRFGRRASFVLFALCSAATVLAYMTLPLDDRVMLVLGFPLGFFASGIFSGM
                     GAFLTELFPSRVRGSAQGFCYNFGRGIGALFPALVGYLSATLGLGMAIGVFAVAAYGL
                     VLVAVAMLPETRGKELLVYD"
     gene            complement(1425..2243)
                     /locus_tag="VGP50_00875"
     CDS             complement(1425..2243)
                     /locus_tag="VGP50_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEV8025946.1"
                     /translation="MRTDLESGLAVRRACRAGEWRATTAGLAKGFVQGNLVVLPAGVA
                     EEFLEFCRRNPKPCPVIGCSKPGDARIPGLGDDLDIRTDLPRYRIWKKGELVAEATDI
                     RDAWRADSVAIVLGCSFSFEEALLAAGVTLRHIAEGRNVAMYRTSIDCVPAGRFAGPL
                     VVSMRPLRPADAIRAVEITARFPAVHGAPVHIGLPHLIGIDDLTTPDYGDAIEVRGDE
                     LPVFWACGVTPQAVIAAAKPDFAITHAPGAMLVTDLLNAQLVTRERTFAGGEGP"
     gene            complement(2391..3101)
                     /locus_tag="VGP50_00880"
     CDS             complement(2391..3101)
                     /locus_tag="VGP50_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEV8025947.1"
                     /translation="MTGDVKALVFDVFGTVVDWRGSVLRELEALGRAKGVAADWAGFV
                     DDWRRGYQPSLQRVRSGELPWTKLDDLHRMTLDALLEKYRVTGLSAAETEYLNRAWHR
                     LDPWPDAVAGLGRLKRRFIIGTLSNGNVALLVALAKRAGLPWDAVFSAELVQHYKPDA
                     QTYEMPPRLLGLAPGEVMLVASHPNDLAAAATQGLKTAYVPRPLEWGPGAKAYDVAGK
                     RFDMTARDFNHLAELLGA"
     gene            3172..3645
                     /locus_tag="VGP50_00885"
     CDS             3172..3645
                     /locus_tag="VGP50_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025948.1"
                     /translation="MDDPAPPVPPRVPPVEPDLLGSAIFLFAEYLQRIADETPPRDED
                     EDEDEDDDEEEDDEDETPSPINMRDVLDLFAEELGTTVPVTLTLYMRVTALYRLLAAS
                     PSLARLASDSDEGESSLNEDALVAAARLDLHVTRSGAGAVADFDPRQFRDALTSS"
     gene            complement(3632..4309)
                     /locus_tag="VGP50_00890"
     CDS             complement(3632..4309)
                     /locus_tag="VGP50_00890"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HEV8025949.1"
                     /translation="MSTDVATEAADCPRCLKPRALCVCDEVTAIDNRVAVLVLQHPQE
                     QDRLLGTARLALAHLKKGQLKIGLSWPSLTKALGRPADPQRWAVLYVGSARAAALAPG
                     RDVVVLDKKGVAAHPDQDDVLKEIQGVVVLDGSWSQAKALWWRNAWMLKSRRILLAPP
                     RPSRYGKLRREPRRDSLATIEAVALVLARLEGRPEIALTLNRSFERMLERYRAARKGG
                     AVPLRSS"
     gene            4521..5777
                     /locus_tag="VGP50_00895"
     CDS             4521..5777
                     /locus_tag="VGP50_00895"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEV8025950.1"
                     /translation="MTTVLASLETTDRQRRALRRNRALATGLLLVAAAVFFATRFFPA
                     PNFWILLLRAGAEAAVVGGLADWFAVTALFRHPLGLPIPRTAIIPKSKDRIGAGLGDF
                     VERNFLAPEIIAAKLRQAQPSRRIAAWLALPSNARLIAEQVASALPYAVRSLDDPDVR
                     GFVLRSFGEQLREIDLAPVLGRVITILTSSGQYDVLFDHLLDAARRLVDAHTHRLYTL
                     VEERSRWWIPKAIDRRIAQAILESIEELLRELREEGGSARAEFRAAVRRLAENLVSSP
                     EQRERFNAAKDRLLEDPDVQHWLADVWDKLRQIVVDDLAAPHSRTREAILTAVLSLGR
                     TLEADLQMQERLDEAIEEAAVAVVPWRGQIGGLIADVVRNWDARTVAERLELAMGSDL
                     QYIRINGTLVGACVGCALFLLSYYLF"
     gene            5789..6487
                     /locus_tag="VGP50_00900"
     CDS             5789..6487
                     /locus_tag="VGP50_00900"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEV8025951.1"
                     /translation="MDIRERLSPNHNERPGPVDMLILHYTGMASAAAALDRLCDPAAK
                     VSAHYVIDEDGTVWRLVPEEARAWHAGVSFWAGRRDINGHSIGIELVNPGHDFGYRPF
                     PEDQMAALETLACAILARHPIPPRHVLGHSDVAPRRKIDPGELFDWPRLARAGIGFWP
                     DFAAKPGRIPVDIPEIHTELAKIGYEIPQNAPADTQITPAITAFQRHFRPSVCDGVPD
                     TETRTRLALVAAGV"
     gene            6521..6898
                     /gene="rnpB"
                     /locus_tag="VGP50_00905"
     ncRNA           6521..6898
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGP50_00905"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1231 a         2661 c         2176 g         1048 t
ORIGIN      
        1 ctcccatttt atttcccctc tcccccatcg ggggagaggg tagggtgagg gggcggcgcg
       61 cgacggatga tcgacacagt cgcgagatat cgccgcaggc gccatgcagc gcccgtctgc
      121 ggcgcgcccc ctcaccccaa ccctctcccc cgatggggga gagggagttg caacgcctgc
      181 atgacgcgct caatcataga ccagcagctc cttcccccgc gtctccggca gcatcgccac
      241 cgccaccagc accaggccat aggccgcgac cgcgaacacg ccgatcgcca tccccaagcc
      301 cagcgtcgcg ctcagatagc cgacgagcgc cgggaagagc gcgccgatgc cgcggccgaa
      361 attgtagcag aagccctggg ccgagccgcg cacgcgcgac gggaagagct ccgtcaggaa
      421 agcgcccatg ccgctgaaga tgcccgacgc gaagaagccg agcggaaagc ccaggaccag
      481 catcacgcgg tcgtcgagcg gcagcgtcat ataggcgagc accgtcgccg ccgagcacag
      541 cgcgaagagc acgaagctcg cgcgccggcc gaagcggtcg gtgatccagg cgctaacgag
      601 atagcccgcg aaagacccga cgatgatgac cgcgagatag gcgcccgagc ccaggaccgt
      661 caggtgccgc acagtgcgca ggaaggtcgg cagccagatg gtgaccgcgt aatacccgcc
      721 ttgcgcgccc gtcgccagca gcgacgcgaa gacggtggtg tagaggagcg gcggcgcgaa
      781 gatctcgaag gaattgagcg tgccgccgac cgccgcgcgc cgccgcgtct cgttgaagac
      841 ctgcggctcc ggcacgaagc gccgcacata gtagacgagc agcgccggcg caaaccccac
      901 ccagaagagg gcgcgccacg cgatcgtttc cggcagcagc ttgaaaaaca gcgtcgccag
      961 gatcgccgcg atgccccagc cgatcgccca ggcgctctgc atcacgccca ccgccctgcc
     1021 gcgatgctcg gccgcgatca tctcgccgac caagaccgag cccaccgccc attcgccgcc
     1081 gaagcccagt ccttgcagcg cccgcgtcgt gaacagcatc tcgaagctgt cggcaaagcc
     1141 gttgaggaac gtgaacaccg cgaaccacag gatcgtcagt tgaagaatcc gcacgcggcc
     1201 gaagcggtcg gccagcatgc ccgcgatcca gccgcccaac gccgacacca ccagcgtcac
     1261 cgtcgcgaga actccggcct cgcccgtgct gatcttccac agcacaagca gcgtcgggat
     1321 gacgaagctg aacaccatga aatccatggc atccattgcc caccccccga acgccgcgac
     1381 gaaggtccgc cgctccgccg ccgtgagccg ccccatccac tccatcatgg cccctctccc
     1441 cccgcgaatg ttctctcgcg tgtcaccagc tgcgcattca gcagatccgt caccagcatc
     1501 gcccccggcg catgggtgat ggcgaaatcc ggcttcgccg ccgcgatcac cgcttgcggc
     1561 gtcacgccac aggcccagaa caccggcagc tcgtcgcccc ggacctcgat cgcatcgccg
     1621 taatccggcg tcgtcagatc gtcgatgccg atgagatgcg gcagcccgat atggacgggc
     1681 gcgccatgca cggcgggaaa gcgcgccgtg atctcgaccg cgcggatcgc atccgccggc
     1741 cggagcggcc gcatcgacac gacgagcgga cccgcaaagc gccccgccgg cacgcagtcg
     1801 atgctcgtcc gatacatcgc gacattacgc ccctcggcga tgtgccgcag cgtcacgccc
     1861 gccgcgagca gcgcctcttc gaacgagaac gagcacccca gcacgatcgc caccgagtcc
     1921 gcgcgccacg cgtcgcgaat atccgtcgct tccgcgacga gctcgccctt cttccagatc
     1981 cggtaccgcg gcagatccgt gcggatgtcg agatcgtcgc cgagtcccgg aatccgcgca
     2041 tcgcccggct tcgagcatcc gatgaccggg caaggcttcg gattcctccg gcagaattcg
     2101 agaaactcct ctgccacccc cgccggcagc accacgagat tcccctgcac gaaccccttg
     2161 gcgagtcccg ccgtggttgc gcgccactcg cccgcgcggc acgcccgccg caccgccaac
     2221 ccgctctcca aatccgtgcg caatgaggac gcgatgtgca tggtttgccg gctcatacta
     2281 agcaggatgg catttcgcgg tcgacgcctc aaatcccctc tccccttgtg ggagagggag
     2341 gggcccattg cgaagcaatg ggagggtgag gggagcgcgt gaccaacccc tcacgcgccc
     2401 agcaactccg cgaggtgatt gaaatcgcgc gccgtcatgt cgaagcgctt ccccgcgacg
     2461 tcataggcct tggcgcccgg gccccattcg agggggcgcg gcacatacgc ggtcttcagc
     2521 ccctgcgtcg ccgcggcggc gagatcgttc ggatgcgagg ccaccagcat cacctcgccc
     2581 ggcgccaggc cgagcagccg cggcggcatc tcgtaggtct gcgcatcggg cttgtaatgc
     2641 tgcacgagct cggccgagaa cacggcgtcc cagggcaggc ccgcgcgctt cgccagggcg
     2701 acgaggagcg ccacattgcc gttggagagc gtgccgatga taaagcgccg cttcaaccgg
     2761 ccgagccccg cgaccgcatc gggccaggga tcgaggcggt gccatgcccg gttcagatac
     2821 tcggtctccg ccgcgctcaa ccccgtgacg cgatatttct cgagcagcgc gtcgagggtc
     2881 atgcggtgga gatcatcgag cttcgtccag ggcagctcgc ccgagcgcac gcgctgcagc
     2941 gacggctgat agccgcggcg ccagtcatcg acgaagcccg cccaatccgc ggccacgcct
     3001 ttcgcgcgcc ccagcgcctc gagctcgcgc agcacgctgc cgcgccaatc gacgaccgtg
     3061 ccgaagacat cgaacaccag cgctttcaca tcgcccgtca tgagcccgct cctgcttccc
     3121 tcatacccct tatattgaag aaatctgctc cgatgcgcta tgagccgcgc catggacgat
     3181 cccgcgccgc ccgtgccgcc tcgtgtaccg ccggtcgagc ccgaccttct gggctccgcg
     3241 atcttccttt tcgccgaata tctgcagcgc atcgccgacg agacgccgcc gcgcgacgag
     3301 gacgaggacg aggacgagga cgacgacgaa gaggaagacg acgaggacga gacgccgtcg
     3361 cccatcaaca tgcgcgacgt gctcgacctc ttcgccgaag agctcggcac caccgtgccg
     3421 gtgacgctca cgctttatat gcgggtgacg gcgctctacc gcctcctcgc cgcgagcccg
     3481 tcgctggcgc gcctcgcttc cgacagcgac gaaggcgaaa gctcgctcaa cgaggacgcg
     3541 ctcgtcgccg ccgcgcgcct cgatctccat gtcacacgca gcggcgccgg cgccgtcgcc
     3601 gatttcgatc cgcggcagtt tcgcgacgcg ctcacgagct cctgagcggc accgcgccgc
     3661 ctttgcgcgc ggcgcggtag cgctcgagca tccgctcgaa gctgcggttg agcgtgagcg
     3721 cgatctccgg ccgcccttcg agccgcgcca gcaccagcgc caccgcttcg atggtggcga
     3781 ggctgtcgcg ccgcggctcg cggcgcagct tgccgtagcg cgacggccgc ggcggcgcca
     3841 gcaggatgcg ccggctcttc agcatccagg cgttgcgcca ccacagcgcc ttcgcctggc
     3901 tccagctccc gtcgagcacg acgacgccct ggatctcctt cagcacgtcg tcttgatcgg
     3961 gatgcgccgc gacgcccttc ttgtcgagca cgacgacgtc gcgccccggc gccagcgccg
     4021 ccgcccgcgc cgatccgaca tagagcacgg cccagcgctg cggatccgcc ggccgcccca
     4081 gcgccttggt gaggctgggc caggagagcc cgatcttgag ctgccccttc ttcagatgcg
     4141 cgagcgcgag ccgcgccgtc cccagcagcc ggtcctgctc ctgcggatgc tgcagcacca
     4201 gcaccgccac ccggttgtcg atcgccgtga cctcatcgca gacgcaaagc gcgcgcggct
     4261 tgaggcatcg cggacaatcg gccgcttccg tcgcaacgtc ggtggacatg ccgcgcctat
     4321 atagggtccc tgcgccgcgt tcaatcggcg acgcggggcg ctatcatccg ccgcgacatg
     4381 cgacgcggcc ccctcaccct gtccctctcc ccgttgggga gagggaacgc gcaccactat
     4441 cctttcccct ctccccttgg ggagagggag ggggcccatt gcgaagcaat gggagggtga
     4501 gggggatcgc gcagcacgca atgaccaccg ttctcgcctc cctcgaaacc accgaccggc
     4561 aacgccgcgc gctccgccgc aaccgcgcgc tcgccaccgg gctgctgctc gtcgccgccg
     4621 cggtcttctt cgccacgcgc ttcttccccg cgccgaattt ctggatcctg ctgctccgcg
     4681 ccggcgccga agcagcggtc gtcggcggcc ttgccgactg gttcgccgtg acggcgctct
     4741 tccgccatcc gctgggcctg ccgataccgc gcaccgccat catccccaag agcaaggacc
     4801 gcatcggcgc aggcctcggc gatttcgtcg agcgcaactt cctcgccccc gagatcatcg
     4861 ccgcgaagct gcgccaggcg cagccgagcc gccgcatcgc cgcctggctc gcactcccca
     4921 gcaatgcgcg cttgatcgcg gagcaggtcg cgtcggcgct gccttatgcc gtgcgctcgc
     4981 tcgacgatcc tgacgtgcgc ggcttcgtgc tccgctcttt cggcgagcag ctgcgcgaga
     5041 tcgatctggc gccggttctc ggccgcgtca tcaccatcct gacctcgagc ggccaatacg
     5101 acgtgctctt cgaccatctg ctcgatgccg cgcggcgcct cgtcgacgcc catacccatc
     5161 gcctctacac cctcgtcgag gagcggagcc gctggtggat ccccaaggcc atcgaccgcc
     5221 gcatcgccca ggcgatcctc gaaagcatcg aggagctgct ccgcgaactc cgcgaggaag
     5281 gcggctcggc gcgcgccgag ttccgcgccg ccgtgcggcg tctcgccgag aacctcgtga
     5341 gctcgcccga gcagcgcgag cgcttcaacg ccgccaagga ccgcctgctc gaggatcccg
     5401 acgtccagca ctggctcgcc gatgtctggg acaagctgcg ccagatcgtc gtcgacgatc
     5461 tcgccgcgcc ccattcgcgg acgcgcgaag cgatcctgac cgcggtgctg tcgctggggc
     5521 ggacgctcga ggccgatctc cagatgcagg agcggctcga cgaggccatc gaagaagcag
     5581 cggtggcggt cgtgccctgg cgcggccaga tcggcggcct catcgccgac gtcgtgcgca
     5641 actgggatgc gcgcaccgtc gccgagcgcc tcgagctcgc catgggcagc gatctccaat
     5701 atatccgcat caacggcacg ctggtcggcg cctgcgtcgg ctgcgcgctc ttcctcctct
     5761 cctactatct cttctgaggc ggccgcccat ggacattcgc gagcgcttgt cgcccaatca
     5821 caatgagcgc ccgggcccgg tcgacatgct catcctccac tacacgggca tggccagcgc
     5881 cgccgccgcg ctcgaccgct tatgcgatcc cgccgcgaaa gtcagcgcgc actatgtcat
     5941 cgacgaggac ggcacggtct ggcggctcgt gcccgaagag gcgcgcgcct ggcatgccgg
     6001 cgtctccttc tgggccgggc ggcgcgacat caacggccat tcgatcggca tcgagctggt
     6061 gaaccccggc cacgacttcg gctaccgccc gttccccgag gaccagatgg ccgcgctcga
     6121 gaccctcgct tgcgcgatcc tcgcccgcca cccgatcccg ccccgtcacg tgctcggcca
     6181 ttccgacgtc gcgccccggc gcaagatcga tccgggcgag ctcttcgact ggccgcggct
     6241 cgcccgcgcc ggaatcggct tctggcccga ttttgccgcc aaacccgggc gtattcctgt
     6301 ggacatcccc gaaatccaca cggaactcgc caaaatcggc tacgaaatcc cccaaaacgc
     6361 ccccgcagac acccaaatca cgcccgcgat caccgctttc cagcgccatt tccgcccttc
     6421 ggtatgcgat ggcgtgcccg acaccgagac ccggacccgc ctcgccctcg tcgccgccgg
     6481 ggtttgactt caggcatccg gcccccctaa tcatgggctg ccagacggcc ggatggccgc
     6541 tctccgcttc ggcggaggga ggaaagtccg ggctccatgg aaatgcggtg ccgggtaacg
     6601 cccggcgagg gcgaccttag ggaaagtgcc acagaaacac accgccaaac cggctctcgc
     6661 cggaggcaag ggtgaaatgg tgcggtaaga gcgcaccgcg ctgccggcaa cggtggcggc
     6721 agggcaaacc ccaccgggag caagaccaag tagggatggc aggccgcaag gccaggcgcg
     6781 tttccacgcc gccatccggg tcggtcgcgc gaggcgtccg gtaacggacg tcccagagga
     6841 atggtcatct cctgccgcaa ggcagggaca gaacccggct tacaggccgt ctggcgctct
     6901 cctttcatct aaaattttag cgaatacttg ggaacaatac aggaacaagc gttaatttac
     6961 gcgacgcaga attcaacccg tagaggaaca gcttcttctt tggccatagg ggcgcgattc
     7021 ctcggtcaac cataattaat acctttgatc ccaaggtttt actggcattt tccattgacg
     7081 accagtttta cccatgttat cccatgaaat cccaat
//
LOCUS       DASYNS010000029         4365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5312593, whole genome shotgun sequence.
ACCESSION   DASYNS010000029 DASYNS010000000
VERSION     DASYNS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5312593"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2069)
                     /locus_tag="VGP50_00910"
     CDS             complement(<1..2069)
                     /locus_tag="VGP50_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HEV8025952.1"
                     /translation="MSSRVAAAEGIRTTCPYCGVGCGVVAARDGSIKADPDHPANRGR
                     LCSKGAALAETLDPAGRLLHPEIDGRRAGWDEALDLIAQSFTQAIERRGPDAVALYVS
                     GQFLTEDYYVANKLMKGFIGAANIDTNSRLCMASSVAGHIRAFGEDVVPGSYDDIDEA
                     DLVVLVGSNAAWCHPVLFQRLAAAKERRGTRIVVVDPRRTQTAEIADLHLALKPGADV
                     ALFAGLLVHLARSGAVAAEWVRRNAVGFEGALAVAAETAATEADVAALTDLAEADVAT
                     FYGWFGCTERTVTLFSQGVNQSVSGTDKVNAIINCHLATSRIGRPGMGPFSLTGQPNA
                     MGGREVGGLANQLAAHMSFADPADIDRVGRFWNAPRMATRPGLKAVDLFDAVLDGRIE
                     ALWIMGTNPAVSMPRAGRIRAALEACPLVIVSDCWPTDTTKLARIVLPAAGWGEKDGT
                     VTNSERRISRQRAFRAPPGEAQPDWWMLTEVARRMGWADAFPYRGPADIFREHARLSA
                     FENDGRRIFDIGALARLSDDAFDAMEPVRWPLPSGAPGPDDGRLFHDGGFSTPDRHAR
                     FVPTPLRPLAEERCERWPLLLNTGRIRDQWHSMTRTGRVPRLMQHVGEPLLEIHPADA
                     ARFGLADDGLAQIESRYGAATLRVRFAREQRPGEVFVPMHWTDGFSSAGPADRLVGAA
                     VDPVSGQP"
     gene            complement(2066..2407)
                     /gene="nirD"
                     /locus_tag="VGP50_00915"
     CDS             complement(2066..2407)
                     /gene="nirD"
                     /locus_tag="VGP50_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HEV8025953.1"
                     /translation="MTAAWIEIGRIEDVPQRGARRVRMSGVPVAVFRTVDDEIFALVD
                     RCPHRGGPLSEGIVSDRSVTCPLHNWMIDLASGRACEPDVGCAPTVPVRVVEGRILLA
                     LEGTGACGEAA"
     gene            complement(2409..>4365)
                     /gene="nirB"
                     /locus_tag="VGP50_00920"
     CDS             complement(2409..>4365)
                     /gene="nirB"
                     /locus_tag="VGP50_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HEV8025954.1"
                     /translation="GMSVAVVHLMATLMERQLDTAAGQLLQRDLDKRGIAFFTKGETE
                     EILGTERAEGVRLTDGREIPADLVVVAIGIRPNIELGRRASLDVNRGILVGDDMRTSD
                     PHIFAVGECIEHNGQVFGLVAPLWEQAKVCAAWLAGDETAAYVPPPVFTKLKITGIDV
                     FSAGALAAADEADDEITFHDAKRGIYKKLVLREDRIVGSVLYGDVADSSWYLQLLGER
                     ADVGALRDCLVFGQAYARQAEGKASSLPAVADMADGMQVCGCNGVSKGAICQAIAAKG
                     LTTLEAVRAHTKASASCGQCTGTVQALLVHMLGAEAAAAASNREPAMCSCTECGHDEV
                     RRAILAGALKSIPAVRQALGWKTADGCHKCRPALNYYLLCSWPGEYRDDRQSRFVNER
                     VHANIQKDGTYSVVPRMWGGLTTPAELRAIADAAEKFQIPTVKITGGQRIDLLGVKKE
                     QLPAVWGDLIKAGLVSGHAYAKGLRTVKTCVGSEWCRFGTQDSTGLGVKIEKMCWGSW
                     TPHKVKLAVSGCPRNCAEATIKDIGVVCVEAGYDIVVGGNGGVDVRVTDHLVRVAGEA
                     EVLEYVGAFLQLYREEARYLERTAPWVARTGIDIIKKRIVEDEENRRRLHERFLYAQR
                     FVQIDPWAERAERNVDAHEFAPLAEVG"
BASE COUNT          687 a         1507 c         1514 g          657 t
ORIGIN      
        1 ggctgccccg agacgggatc cacggccgcg ccgaccagac ggtccgccgg cccggcggag
       61 ctgaagccgt cggtccagtg catcggcacg aagacctcgc ccggccgctg ctcgcgcgcg
      121 aagcgcacgc gaagcgtcgc cgcgccgtac cgactctcga tctgcgccag accgtcatcg
      181 gcgagaccga agcgggcggc gtcggcgggg tggatctcga gcaacggctc gccgacatgc
      241 tgcatgaggc gtgggacccg ccccgtccgc gtcatactgt gccattggtc gcggatgcgc
      301 ccggtattga gcagaagcgg ccagcgctcg cagcgctcct cggcgagcgg gcgcaacggc
      361 gtcggcacga agcgcgcatg acggtcgggc gtcgaaaacc cgccgtcatg gaagaggcgg
      421 ccgtcgtccg ggccgggcgc gccgctcggg agcggccagc gcaccggctc catcgcgtcg
      481 aaggcatcgt cgctcaatcg ggcgagagcg ccgatatcga agatgcggcg cccgtcattc
      541 tcgaaagcgg agagacgcgc gtgctcgcga aagatgtccg ccggcccgcg atacgggaag
      601 gcatcggccc agcccatgcg gcgcgcgact tccgtcagca tccaccaatc gggctgcgcc
      661 tcgccgggcg gcgcacgaaa cgcgcgctgg cgcgagatgc gccgctcgga attggtgacg
      721 gtgccgtcct tttcgcccca gccggccgcc ggcagcacga tgcgggcgag cttcgtcgtg
      781 tcggtcggcc agcaatcgga gacgatgacc agcgggcaag cctcgagcgc cgcacgaata
      841 cgcccggcgc ggggcatgct gacggcggga ttggtgccca tgatccagag cgcctcgatg
      901 cggccgtcga gtacggcgtc gaagagatcg acggccttca ggcccggtcg tgttgccatg
      961 cgcggcgcgt tccagaagcg cccgacacgg tcgatatcgg ccgggtcggc aaagctcata
     1021 tgcgcggcca gctgattggc gagaccgccg acctcgcggc cgcccatggc attgggctgc
     1081 cccgtgagcg agaagggccc catcccgggc cggccgatgc gcgacgtggc gagatggcag
     1141 ttgatgatgg cgttgacctt gtcggtgccg gagacggact ggttcacgcc ttgcgaaaag
     1201 agcgtgacgg tgcgctcggt gcagccgaac catccgtaga acgtcgcgac gtccgcttcg
     1261 gcgaggtccg tcagcgcggc gacgtcagct tccgtcgctg cggtctcggc cgcgaccgcc
     1321 agcgctccct cgaaaccgac ggcgtttcgc cggacccact cggccgcaac cgcacccgac
     1381 cgcgccagat gcaccagcag gccggcaaag agagcgacgt ccgcgccggg cttcagcgcg
     1441 agatgcagat cggcgatctc tgcggtctga gtgcgccgcg gatcgaccac gacgatgcgc
     1501 gtcccgcgcc gctccttggc ggcggcgagg cgctgaaaca gcaccgggtg acaccaggcg
     1561 gcgttgctgc cgaccaggac gacgaggtcc gcctcgtcga tgtcgtcgta gctgccggga
     1621 acgacgtcct cgccgaaggc ccggatgtgc ccggcgacgg aagaggccat gcacaggcgc
     1681 gagttggtat cgatgttcgc ggcgccgatg aagcccttca tcagcttgtt ggcaacgtag
     1741 tagtcctcgg tgaggaactg tcccgagacg tagagcgcca cggcgtcggg gccgcgccgc
     1801 tcgatggctt gcgtgaagct ctgcgcgatc agatcaagcg cctcgtccca gccggcgcgg
     1861 cggccgtcga tctcgggatg aagcagccgt cccgccgggt cgagcgtctc ggcgagcgcc
     1921 gcacccttcg agcagagccg gccgcgattg gccggatgat cggggtcggc cttgatcgac
     1981 ccgtcgcgcg cggccaccac gccgcagccg acgccgcaat aggggcacgt cgtgcgtatg
     2041 ccctccgccg cggcgacgcg gctgctcacg cggcttcccc gcacgcgccg gtgccctcga
     2101 gagctagcag gatgcggccc tcgaccaccc tcaccggcac ggtcggtgcg caaccgacat
     2161 cgggctcgca cgcccggccg ctggcaaggt cgatcatcca gttgtgcagc gggcacgtca
     2221 ccgagcggtc cgagacgatg ccttcggaca atggcccgcc gcgatgggga cagcgatcga
     2281 cgagggcgaa gatctcgtcg tcgaccgtgc ggaagaccgc gaccggaacg ccgctcatgc
     2341 gcacgcggcg ggcgccgcgc tgcggcacgt cctcgatgcg gccgatctcg atccacgctg
     2401 ccgtcatctc agccgacctc cgccagcggc gcaaactcgt gcgcatccac gttgcgctcg
     2461 gcgcgctcgg cccaggggtc gatctggaca aaacgctgcg cgtagaggaa gcgctcatgc
     2521 agccggcgcc ggttctcctc gtcttcgacg atgcgcttct tgatgatatc gatgccggtg
     2581 cgagcgaccc agggcgcggt gcgctcgaga tagcgcgcct cctcgcggta gagctgcagg
     2641 aaggccccga catactccag gacctcggcc tcgccggcga cgcgaaccag atgatcggtg
     2701 acgcggacgt cgacgccgcc attgccgccg acgacgatgt cgtagcccgc ttcgacgcaa
     2761 accacgccga tgtccttgat ggtcgcctcg gcgcaattgc gcggacagcc ggagaccgcg
     2821 agcttgacct tgtgcggcgt ccacgagccc cagcacatct tctcgatctt gacgccgagg
     2881 ccggtcgagt cctgcgtccc gaagcggcac cattcgctgc cgacgcaggt cttcaccgtg
     2941 cgcaggccct tggcataggc gtgtcctgaa acgagcccgg ccttgatgag gtcgccccag
     3001 accgccggca attgctcttt cttgacgccg agcaggtcga tgcgctgtcc gcccgtgatc
     3061 ttcacggtcg gtatctggaa cttctccgcc gcatcggcga tggcccgcaa ctcggccggc
     3121 gtcgtgagcc cgccccacat ccgcggcacc accgagtagg tgccgtcctt ctggatgttg
     3181 gcgtggaccc gctcgttgac gaagcgggac tgccggtcgt cgcggtattc gccgggccaa
     3241 gagcagagga ggtaatagtt gagcgccggg cggcatttgt ggcagccatc cgccgtcttc
     3301 cagccgagcg cctggcgcac cgccggaatc gatttgagcg cgccggcgag gatggcgcgg
     3361 cggacctcgt catggccgca ctcggtgcag ctgcacatcg ccggctcgcg gtttgacgcc
     3421 gccgccgccg cttcggcgcc gagcatgtgg acgagcagcg cctggacggt gccggtgcat
     3481 tggccacagg acgcggacgc tttggtgtgg gcgcgcaccg cctcgagcgt cgtcagcccc
     3541 ttcgcggcga tggcctggca gatggcacct ttggagacgc cgttgcagcc gcagacctgc
     3601 atgccgtcgg ccatgtcggc gacggcgggg agggaagacg ccttgccctc ggcctggcgc
     3661 gcataggcct gtccgaagac caggcagtcg cgcaacgcgc cgacatcggc gcgctcgccc
     3721 agcaattgca gataccagga actgtcggcg acatcgccat agagaaccga gccaacgatc
     3781 ctgtcctcgc gcagcaccag cttcttgtag atgccgcgct tggcgtcgtg gaaggtgatc
     3841 tcgtcatcgg cctcgtcggc tgccgccagc gctcccgccg agaacacgtc gatgccggtg
     3901 atcttgagct tggtgaagac gggcggcggc acataagcgg cggtctcgtc gcccgcgagc
     3961 catgcagcgc agaccttggc ctgctcccag agcggcgcta ccaggccgaa gacctggccg
     4021 ttgtgctcga tgcactcgcc gacggcgaag atgtggggat cgctggtgcg catgtcgtcg
     4081 ccgaccagaa tgccgcggtt gacgtcgagg ctggcgcggc gtccgagctc gatgttgggc
     4141 cggatgccga tggcgacgac cacgagatcg gcgggtattt cgcggccgtc cgtcaggcgg
     4201 acgccttccg cccgttcggt gccaagaatc tcctcggtct cgcccttcgt gaagaaagcg
     4261 atgccgcgct tgtcgagatc gcgctgcagc agctggccgg cggccgtatc gagctgacgc
     4321 tccatgagtg tcgccatcag gtgcacgacc gcgaccgaca tgccg
//
LOCUS       DASYNS010000030         6817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_944387, whole genome shotgun sequence.
ACCESSION   DASYNS010000030 DASYNS010000000
VERSION     DASYNS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944387"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /locus_tag="VGP50_00925"
     CDS             complement(<1..958)
                     /locus_tag="VGP50_00925"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV8025955.1"
                     /translation="MNEADELRQAKAFSEKAFAAMTAHGVPPTPENFTVWYAYAVGLV
                     PDLKRAIDLMIANQDAFSQTVNSDLYNRFCNIGEHFNLLQETGDGLYHAADQLIHYVK
                     TASGDTQAFGKTLDRYSTELGAAPGGDKLRTMVSGLILETQRVAERNRILEAQLNESS
                     SEIQQLRQNLELVRRESLTDALTGIPNRKFFDTRLRAAVLDANKTGEPLSLVITDIDH
                     FKHFNDTYGHQMGDQVLRLVGRTLTDSVKGRDTPARYGGEEFALILPQTRLADALVLA
                     EQIRRTIMRRHIVRRDTGDDFGTITLSMGVAAYRPTEHLTDTL"
     gene            complement(1043..1807)
                     /locus_tag="VGP50_00930"
     CDS             complement(1043..1807)
                     /locus_tag="VGP50_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8025956.1"
                     /translation="MSDRRRVLPLQAGVFALLVGAWQALAASGAIDPFFFSRPSAIAW
                     QIWRWMASGYIWPHLFVTLAEALLAFVIGTASGIVAGLAFARIELLAAVFDPYIRMLN
                     ALPRVILAPIFILWFGVGMASKVALGVTLVFFIVFFNTFQGIREVDPVLRDNARMLRA
                     SNRQMLRHVDLPSAMAWIFASLHTSIGFALVGAVVGEYIGAARGIGYVVAQAQGVFDT
                     TGVFAGLILTAAVVLCIDLAIHRLERHLLRWRPGRT"
     gene            complement(1804..2598)
                     /locus_tag="VGP50_00935"
     CDS             complement(1804..2598)
                     /locus_tag="VGP50_00935"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8025957.1"
                     /translation="MEKAVEFARVGVHFAAPRGGRHSVLRDVSLEIAEGTFVAIVGPS
                     GCGKSTLLNLAAGLLAPADGGVRVFGESLGGINRHAGYLFQQDALLPWRTVLDNVRLG
                     LDFRRVAPAEAIAKARSFIARVGLAGFEDRFPYQLSGGMRKRVAIAQSWIVEPRILLM
                     DEPFAALDVQTRQHMENELLGLWQESRKTVLFVTHDLEEAIALADRVVVLTVGPARVK
                     GVYDIDLPRPRDVAEIRLQSDFIALYRRIWSDLRDEVRHAFATGER"
     gene            complement(2735..3832)
                     /locus_tag="VGP50_00940"
     CDS             complement(2735..3832)
                     /locus_tag="VGP50_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8025958.1"
                     /translation="MTIKAARISMRLAGALAVLVAAGAMGGGAGGGGMGGGMMGDPFR
                     HTGTPPHVKIAVGGAACLCYLPTVLAQRLGAYEKAGVDVELINVKGGSTALAAVLGGS
                     ADAVSGYYDHCVNLAAKDKALKAFVTYDRLPGLVLVVAPGQSAAIKSVRDLAGKKVGV
                     SAPGSSTDFFLKYLLAKAGVDPAQVAVIGIGLDATAVAAMEQGEVDAAVMLDPAVTLL
                     QGKYRDLRLLDDTRSEGDTRKVFGGDYPGGALYAQSQWVSDHPKETQALTDAIVTTLG
                     WIHDHSAEEIMAKMPDEMIGPDRQLYLAALKNTLPMYSKTGLMDPKGADAVLAVFSQS
                     SPEVANAHIDVAKTYTNDFASRADAKLGLTQ"
     gene            3973..4344
                     /locus_tag="VGP50_00945"
     CDS             3973..4344
                     /locus_tag="VGP50_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025959.1"
                     /translation="MLRTFLRRRQRRTDTAANTAGLLHRLESEIAQEQALQFGAAAIG
                     FIGAILGLTVSSAFVLLPAFAIATLGQYLLQGWCPPLALLARLGLRSSAEIDSERYAL
                     TASLRRTREPHFSATAVAEAD"
     gene            complement(4381..>6817)
                     /locus_tag="VGP50_00950"
     CDS             complement(4381..>6817)
                     /locus_tag="VGP50_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEV8025960.1"
                     /translation="SKQFRQLQEKGEWDGCVEMAREQVKEGSHALDVCTAFVGRDEIA
                     DMTQVLSRMRGSVNAPLVIDSTELPVLEAALKLYGGKPVLNSINFEDGETPAAKRLEL
                     ARRFGTAVVALTIDEQGMAKDVERKVALAKRLHDFACVRHGLPASDLMFDPLTFTICT
                     GNEDDRKLGLWTLDAIERIAKELPECQIILGLSNISFGLNPAARHVLNSVFLDHALRR
                     GLTGAIVHISKIMPLHKIPETELNAAEDLIFDRRRPGHDPLHAFMALFENRVVAKAVR
                     KERPAAVEERLKQRIVDGERQGLEDDLKLAMEKHKPLDIINIFLLDGMKVVGELFGAG
                     KMQLPFVLQSAETMKAAVAYLEPHMERLEGQQKGTIVLATVKGDVHDIGKNLVDIILT
                     NNGYRVVNLGIKQPIAAILDAASQHKADAVGLSGLLVKSTVVMRENLEEMARQGITTP
                     VMLGGAALTRRYVEEDCVKAYACGRVAYARDAFDGLDLMEKVVNGRFDAHLSEIATKN
                     RGRVVNEKRKLGRAADARPLRPVDVEEIRLRRAELTHGLPVPQPPFWGPRSIARVPVK
                     ALVPYLNERMLYQFHWGFRKDGRTLDEFKAWAHKELRPIMQRILDTAIQQDILVPQAA
                     YGYWKCAADGNDVIVFAEDGIKEVARFSFPRQNKEGGLCIADFFRDVTDNERDVIAMQ
                     VVTMGPRASATAQEWFADNRYKDYLYLHGLSVEMAEAMAEYLHKRIRAELGFTAEEAR
                     DIEALQQQGYRGSRYSFGYPACPNLGDQRPLLALLNAEEIGVALTEEDQLDPEQSTSA
                     IVVTHPQAKYFSV"
BASE COUNT         1155 a         2215 c         2279 g         1168 t
ORIGIN      
        1 cgagcgtgtc cgtcagatgc tcggtgggac gataggcggc gacgcccatc gagagcgtga
       61 tggtgccaaa atcgtcgccg gtgtcgcggc gcacgatatg gcggcgcatg atggtgcggc
      121 ggatctgctc ggcgagcacc aacgcgtccg caagccgggt ctgcggcagg atcagagcga
      181 attcctcgcc gccgtagcgt gccggcgtgt cgcggccctt gacgctgtcg gtcaatgtcc
      241 gcccgacgag gcgcagcacc tgatcgccca tctgatggcc gtaggtgtcg ttgaaatgct
      301 tgaaatgatc gatgtcggtg atgaccagcg agagcggctc gccggttttg ttggcatcga
      361 gcacggcggc gcgcagccgc gtgtcgaaga atttgcgatt gggaatgccg gtgagcgcgt
      421 cggtcagcga ctcgcgccgc accagctcga ggttctggcg cagctgctgg atctcgctcg
      481 aggactcgtt gagctgagct tcgaggatgc ggttgcgctc ggcgacgcgc tgggtctcga
      541 ggatgaggcc ggagaccatc gtccgcagct tgtcgccgcc gggagcggcg ccgagctcgg
      601 tcgaatacct gtcgagggtc ttaccgaagg cctgagtgtc gcccgacgcc gtcttgacgt
      661 aatggatgag ctggtcggcg gcgtgataga ggccgtcgcc ggtctcctgc agcagattga
      721 aatgctcgcc gatgttgcag aaacggttgt agagatcgct gttgaccgtc tggctgaacg
      781 cgtcctgatt cgcgatcatc aggtcgatgg cgcgtttgag gtccggcacc aatcccacgg
      841 cataggcgta ccagaccgtg aaattttccg gcgtcggcgg cacgccgtga gcggtcatgg
      901 cggcgaacgc cttttcggag aacgcctttg cctgacggag ctcgtcggct tcgttcatcg
      961 tacccgcttt cattttagac aaaacgaccg cactcgtagc cctagagcgt gccagagttt
     1021 tcttgcgaaa tggttgctgg tgctatgtcc gcccaggccg ccagcgcagc aggtggcgct
     1081 cgagccggtg gatcgcgaga tcgatgcaca gcaccaccgc cgcggtgagg atgagcccgg
     1141 caaagacgcc ggtcgtgtcg aagacgccct gtgcctgcgc cacgacataa ccgatgccgc
     1201 gcgcggcgcc gatatattcg ccgaccacgg cgccgaccag cgcgaagccg atgctggtat
     1261 gcaggctcgc gaagatccag gccatggccg agggcaggtc gacatggcgc agcatctgcc
     1321 gattgcttgc ccgcagcatg cgcgcgttgt cgcgcaggac gggatcgacc tcgcggatgc
     1381 cctggaaggt gttgaagaag acgatgaaga ataccagcgt aacgccaagc gcgaccttgg
     1441 aggccatgcc gacaccgaac cagaggatga agatcggcgc gaggatcacg cgcggcagcg
     1501 cgttgagcat acggatgtag ggatcgaaca ccgccgccag cagctcgatg cgggcgaaag
     1561 caaggcccgc gacaatcccc gaagccgtgc cgatgacgaa ggcgagcagc gcttcggcga
     1621 gcgtcacgaa gaggtgcggc cagatatatc cgctggccat ccagcgccag atctgccagg
     1681 cgatcgccga ggggcggctg aagaagaagg gatcgatggc gccgctcgcc gccagcgcct
     1741 gccaggcgcc gaccagcagc gcgaataccc ctgcctgcag cggcagcacc cgccgccggt
     1801 cgctcatctc tcgcctgtcg cgaaagcgtg cctcacctca tcgcgcagat cgctccagat
     1861 tcggcgatag agcgcgatga aatccgattg caggcgaatc tcggccacgt cgcgcggccg
     1921 cggcagatcg atgtcgtaga cgcccttgac gcgcgccggc ccgaccgtca gcacgacgac
     1981 gcggtcggcg agcgcgatgg cttcctcgag atcgtgggtg acgaagagca cggtcttgcg
     2041 gctctcctgc cacaggccaa gcagctcgtt ctccatgtgc tggcgcgtct gcacatcgag
     2101 ggcggcgaag ggttcgtcca tcagcaggat gcgcggctcg acgatccagc tctgggcgat
     2161 ggcgacgcgc ttcctcatcc cgcccgagag ctggtagggg aagcggtctt cgaagcccgc
     2221 gagaccgacg cgggcgatga aggagcgcgc cttggcgatc gcctccgccg gcgcgacgcg
     2281 gcggaaatcg aggccgaggc gcacattgtc gagaacggtc cgccagggca ggagcgcgtc
     2341 ctgctgaaag agatagccgg cgtgccggtt gatgccgccg agcgactcgc cgaacacgcg
     2401 cacgccgccg tccgccggag cgagcaaccc tgccgccaga ttgagcaggg tcgatttgcc
     2461 gcagcccgag ggcccgacga tggcgacgaa ggtgccctcc gcgatctcga gcgatacgtc
     2521 gcgcaacacg ctatgccggc cgccgcgcgg cgccgcgaaa tgcacgccga cgcgcgcgaa
     2581 ctcgacggcc ttttccacct ctaacagcga ccgccggcgg ctgcttcacc ccccacccta
     2641 ccctccccct caagggggga gggaaaagaa aaagcggcac cacgcaatcc cctcccccct
     2701 tgtgggggag ggttagggtg gggggtccac agcgtcactg cgtcagaccg agcttcgcgt
     2761 cggcgcgcga agcgaagtcg ttggtatagg tcttggcgac gtcgatatgg gcgttcgcta
     2821 cctcgggcga cgactggctg aagacggcga gcacggcgtc ggcgcccttg ggatccatga
     2881 gcccggtctt cgaatacatc ggcagcgtat tcttgagcgc ggcgagatag agctggcggt
     2941 cgggcccgat catctcgtcg ggcatcttcg ccatgatctc ttcggcgctg tggtcatgga
     3001 tccagccgag cgtcgtgacg atcgcatcgg tcagcgcctg ggtctccttc gggtgatcgc
     3061 tcacccattg gctctgcgca tagagcgcgc cgcccggata gtcgccgccg aacactttgc
     3121 gcgtgtcgcc ttcgctgcgc gtgtcgtcga ggaggcgcaa gtctcggtat ttcccttgca
     3181 gcagcgtcac tgccggatcg agcatgaccg cagcatcgac ctcgccttgc tccatggcgg
     3241 cgacggccgt cgcatcaagg ccgatgccga tcaccgccac ctgcgccgga tcgacgccgg
     3301 ctttggcgag gagatatttg aggaagaaat cggtcgagga gccgggcgcg ctgacgccga
     3361 cttttttccc ggcgagatcg cggaccgatt ttatcgccgc gctctgtccg ggcgcgacca
     3421 cgagcaccag gccaggcaag cggtcatagg tgacgaaggc tttgagcgcc ttgtccttgg
     3481 ccgcgagatt gacgcaatgg tcgtagtagc cggagacggc gtcggcgctg ccgccgagca
     3541 cggcggcgag cgccgtcgag ccgcccttga cattgatcag ctcgacatcg acgccggcct
     3601 tctcataggc gccgagccgt tgcgcgagca ccgtgggcag atagcacagg caggcggcac
     3661 ccccgaccgc gatcttgaca tggggcggcg tcccggtgtg ccggaacggg tcgcccatca
     3721 taccgccgcc catcccgcca ccgcccgcgc ccccgcccat ggctcccgcg gcgacgagca
     3781 ccgccagggc gccggccagc cgcatcgaga ttcgcgcagc tttgatcgtc atggtctcct
     3841 cctggcttac cctgattccg ccagcttagc ctatgcctgc ggaacaggtg aaatggacac
     3901 tgccattaac ggcgcatcag ggatcccttg gcatcatgcg tgcgaaccca cgccctgaag
     3961 gaaaccctcg acgtgttgcg aactttcctg cgccgccggc agcggcggac ggacaccgct
     4021 gccaatacgg cggggctgct tcaccggctc gagagcgaga tcgcgcagga gcaggccctg
     4081 caattcggcg ctgccgccat cggcttcatc ggcgcgatcc tgggcctcac ggtgagcagc
     4141 gctttcgtcc tgctgccggc tttcgccatc gccacgctcg gtcaatatct gctgcagggc
     4201 tggtgcccgc cgctcgcgct cctcgcgcgg ctcggcctgc gcagcagcgc agagatcgac
     4261 agcgagcgct acgcgctcac agcatcgctg cgcagaaccc gcgagcccca tttctccgcc
     4321 acggccgttg ccgaggccga ctgagcgcgg cgcagaaccg gcttcgcgcc cggtctcgga
     4381 tcagaccgag aaatacttcg cctgtggatg cgtcacgacg atcgccgagg tcgactgctc
     4441 gggatcgagc tgatcctcct cggtgagcgc gaccccgatc tcctcggcat tcaacaacgc
     4501 cagaagcggc cgctgatcgc cgaggttcgg acaggccgga tagccgaagg aatagcggct
     4561 gccgcgatag ccctgctgct gcagcgcctc gatgtcgcgc gcctcctcgg cggtgaagcc
     4621 gagctcggcg cggatgcgct tatggagata ttcggccatc gcctcggcca tctccaccga
     4681 gaggccgtgg agatagagat agtccttgta gcgattgtcg gcgaaccatt cctgcgccgt
     4741 cgccgaggcg cgcggcccca tcgtcaccac ttgcatggcg atgacgtcgc gctcgttgtc
     4801 ggtgacgtcg cggaagaaat cggcgatgca gagcccgcct tccttgttct gccgcgggaa
     4861 ggagaagcgc gccacttcct tgatgccgtc ctcggcaaag acgatgacgt cgttgccgtc
     4921 ggcggcgcat ttccagtaac cgtaggccgc ctgcggcacc aggatgtcct gctgtatcgc
     4981 cgtgtcgagg atgcgctgca tgatcggccg cagctcttta tgcgcccagg ccttgaactc
     5041 atcgagcgtg cgcccatcct tccggaaccc ccaatggaat tggtagagca tgcgctcgtt
     5101 gagatacggg accagcgcct tcaccggcac gcgggcgatg ctgcgcggcc cccagaaggg
     5161 cggctgaggc accggcaggc cgtgcgtcag ctcggcgcgg cgcagccgga tctcctcgac
     5221 atcgaccggg cgcagcgggc gcgcatcggc ggcgcggccg agcttgcgct tctcgttgac
     5281 gacgcggccg cggttcttgg tcgcgatctc cgacagatgc gcgtcgaaac ggccgttgac
     5341 caccttctcc atgagatcga ggccgtcgaa ggcgtcgcgc gcataggcga cgcggccgca
     5401 agcataggcc ttgacgcaat cctcctcgac atagcggcgc gtcagcgcgg cgccgcccag
     5461 catcaccggc gtggtgatgc cctggcgcgc catctcctcg agattctcgc gcatgaccac
     5521 ggtcgacttg acgagcaggc ccgagagccc gacggcgtcc gccttgtgct gtgacgccgc
     5581 gtcgagaatg gcggcgatcg gctgcttgat gccgagattg acgacgcgat agccgttgtt
     5641 ggtgaggatg atgtcgacga ggttcttgcc gatgtcgtgg acgtcgcctt tcaccgtcgc
     5701 cagcacgatc gtgcctttct gctggccttc gaggcgctcc atgtgcggtt cgagataggc
     5761 gaccgcggcc ttcatggtct cggccgattg cagcacgaag ggcagctgca tcttgccggc
     5821 gccgaagagc tcgcccacca ccttcatgcc gtcgagcagg aagatgttga tgatgtcgag
     5881 cggcttgtgc ttctccatcg ccagcttgag gtcgtcttcg aggccttgac gctcgccgtc
     5941 gacgatgcgt tgcttcaagc gttcctcgac ggccgccgga cgctccttgc gcacggcctt
     6001 ggcgacgacg cggttctcga agagggccat gaaggcgtgg agcggatcat ggccgggacg
     6061 gcgccggtcg aagatcaggt cctcggcggc attgagttcg gtttccggga tcttgtgcag
     6121 cggcatgatc ttcgagatgt gcacgatggc cccggtgagg ccgcgccgca aggcatggtc
     6181 gaggaacacc gagttcagca catggcgggc cgcgggattg aggccgaagg agatgttgga
     6241 gaggccgaga atgatctggc actccggcag ctccttggcg atgcgctcga tcgcgtccag
     6301 cgtccagagc ccgagcttgc ggtcgtcctc attgccggtg cagatggtga aggtgagcgg
     6361 atcgaacatc aggtccgacg ccggcaggcc gtgacgcacg caggcgaaat catggagacg
     6421 cttggcgaga gcgaccttgc gctcgacgtc cttggccatc ccctgctcgt cgatggtaag
     6481 cgcgacgacg gccgtgccga agcgccgcgc aagctcgagg cgcttcgccg ccggcgtctc
     6541 gccgtcctcg aagtttatcg agttgagcac gggcttgccg ccatagagct tgagcgccgc
     6601 ctcgagcacc ggcagctcgg tcgagtcgat gaccagaggg gcattcaccg agccgcgcat
     6661 ccggctcagc acctgagtca tgtcggcgat ttcgtcgcgg ccgacgaagg cggtgcagac
     6721 atcgagggcg tgcgagcctt ccttcacctg ctcgcgcgcc atctcgacgc agccgtccca
     6781 ctcgcctttc tcctggagct ggcggaattg cttcgac
//
LOCUS       DASYNS010000031         7508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5001306, whole genome shotgun sequence.
ACCESSION   DASYNS010000031 DASYNS010000000
VERSION     DASYNS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5001306"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VGP50_00955"
     CDS             <1..517
                     /locus_tag="VGP50_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV8025961.1"
                     /translation="SLAAATAAIGIETAQLYPQVSIGGALGIAGPFSTIGSVSSFGGS
                     VGPLLSWTFPNISVAHARIAEAGATAEAAEARFDGAVLAALQETETALDAYVREIDHD
                     HALKEARGSAEKASDQARALFRFGRTGILDVLNAEAALATAETALAASDAAIADSEVN
                     VFLALGGGWEP"
     gene            651..1292
                     /locus_tag="VGP50_00960"
     CDS             651..1292
                     /locus_tag="VGP50_00960"
                     /inference="COORDINATES: protein motif:HMM:NF037152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8025962.1"
                     /translation="MTCRSQTYERIVLKASELLRRFGLRKTAVADIAHELGMSPANIY
                     RFFPSKRALVEAVAERRLMVLRQDLAGVVRSRKTAFARLEDLVRAVASSFRTMCAQRD
                     LLEIELARDLFEIEGMRRESQWQFVADFHDFLRAELTKLIRAGVAAGEMRVADPADTA
                     AAVFDGFIWVIEPLMLLRDPEPVAEQRLERQFRLLARALGVSTLAGEVTNVAE"
     gene            1336..2490
                     /locus_tag="VGP50_00965"
     CDS             1336..2490
                     /locus_tag="VGP50_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV8025963.1"
                     /translation="MALLPLVLYGCSRKPAAQAAAPPPQVGVVTLQPEPVNRTTELPG
                     RTSAFLTSDVRPQVGGVVLKRLFTEGSDVQEGQQLYQIDPAPYQATYESAVATLAHAE
                     AALADANAKTARYKPLVEAKAVSKQDYDDAVASAREAEADIKSAHAAIDQARINLDYT
                     KVLAPIAGTIGRSIVTPGALVTASQALVLATVTQLDPIYVDVNQPVATLLRFERELAA
                     GEIERADDGAAKVTLELEDGSAYPLPGKLEFHEVNVNQSTATVVVRALFPNPKHLLLP
                     GMYVHAEIEEGVKANGILVPQAAVSRNPRGDGIVMVVGEDGKASSRVVATGPAVGDRW
                     VVASGLNPGERVIVDGLQKVRPGMPVQPVAAGSSVAEAPNSRTSGSGSSR"
     gene            2487..5648
                     /locus_tag="VGP50_00970"
     CDS             2487..5648
                     /locus_tag="VGP50_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011252265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV8025964.1"
                     /translation="MAMSRFFIDRPIFAWVLGLVLMLAGAIEIFLLPVAQFPSIAAPQ
                     VSITVVYPGASAQTVQDTVIQPILQQMSGLDGLEYIASTARADGSTEIDFTFVQGTDP
                     NIAQVQVQNNLSLAEALLPAVVTQQGIKVKKSVKNFMLVVALVSTDGSMSGPDISDYI
                     ASHLQDPLTRIPGVGDFTLFGSEYAMRIWLDPDKLYKYSMTVVDVANAIAAQNVQVSS
                     GELGGLPASKGQRLDATIIGPARFETPEQFANILLRVNQDGSQVRLRDVARVEIGAQQ
                     YTPTALLNGEPTSALALKLSPGANQLTTEAAVKAELGRLAQFFPPGIEIVYPLDTEPF
                     ITLSLTEVAKTLLEAIALVFVVIFVFLQDFRATLIPTIAVPIVLLGTFAVLAGFGYSI
                     NTLTMLAMVLAVGLLVDDAIVVVENVERVMAERGLSPREAARQSMDEISGALIGIALV
                     LSAVFLPMAFFSGSTGVIYRQFSVTIISAMTLSVLVALILTPALCATLLKHKPQGEAG
                     GAKRLFGWFNRGFDWTNRRYGGGVSRMIAHSRWSLIAFAALTVLAVLLFARMPTGFLP
                     DEDQGLLFGQVTLPPGATAEETTAVNKEVADYLLSQEKDSVEDVLSVTGFNFAGQAQN
                     AGFLVIKLRPWRERPKPSEWAGAVAGRTTRRFFGARDAQIFVFNLPAVLELGNATGFD
                     LELEDRAQLGHEKLLAARNQLLGMAAQDHSLFAVRPNGLEDAPQFHLDIDREKADALG
                     VSISDLDATVQGALGSQFVNLFTRNGRVKQVYVQGEPDSRMLPSELSRWYVRNGTGDM
                     VPFDAVLTGSWTLGPQKVEGYNEVPSFEIQGQPAPSGSTGAAMATLERMIQKLPPGVG
                     YEWTGLSYEEQKAGSQTGPLYAISLTVVLLCLAALYESWPVPISVLLVVPLGVVGAIC
                     ATLFRGLDNDVYFQVGLLTTVGLATKNAILIVEFAKAFFDSGMPLVEAAKQAAHERLR
                     PILMTSIAFVCGTLPLTIATGAGAASRIAIGTAVVGGMLSATVLAIFFVPVFFVAILS
                     LFRVKPRPRPSVAQKAPVAGQEA"
     gene            5652..7178
                     /locus_tag="VGP50_00975"
     CDS             5652..7178
                     /locus_tag="VGP50_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEV8025965.1"
                     /translation="MRRSRLTIALLAGLAGCSLIPDYHRPALPVSEQWPGGPAAEEAA
                     RAVPAGAPPAAALGWRDFFADPAMQDLIALTLENNRDLRVAALNVIAAEAQYRVDRAS
                     LFPQVDATAALQRSRTPASVEGLAGITGISALNVREYSLGLSTVSWELDFFGQIRSQA
                     KQAQETYVSNAETEISTRIALIAEVTSEYLTWLADRDSLAVSEDTVKVQADSLQLTEQ
                     RSARGTATDQDVAQAETTLRTAEASAAQFTRQVAQDMDELVLLVGAPLPEALVVRMNA
                     EAGLGSQPVFPELPAGVPSDLLERRPDIRAAEHALLAANANIGAARAAFFPQITLTGQ
                     YGTASSDLHHLFGRGSTFWVFDPSISLPIFDAGRNQANLDIAKVEKRIEVANYEKAIQ
                     SAFHDVADALAGRGTYIDEVTAEQGLVDADARFYDLAVMRFRNGVDTYLNVLVAQNAL
                     LSARLTLISLKLAQQQNLVTLYKALGGGWEERSQASASSTPRPSGAGEGATRRAASGG
                     "
     gene            complement(7248..>7508)
                     /locus_tag="VGP50_00980"
     CDS             complement(7248..>7508)
                     /locus_tag="VGP50_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV8025966.1"
                     /translation="VFQDKPHFGVGIVPGGDIQSLWPHVIGSPRGRCFVVAQHRLSAE
                     EAKTLGVVDEIVSRERLLGRAREIAAAIAKLPPLTAALRAWI"
BASE COUNT         1177 a         2635 c         2443 g         1253 t
ORIGIN      
        1 cagcctcgcg gcagcgaccg cagcgatcgg catcgagacg gctcagctct atccgcaggt
       61 cagcatcggc ggtgcgctcg gcattgccgg acctttctcg acgatcggct cggtgtcgag
      121 cttcggcggc agtgtcggac ctctgctgtc ctggactttc cccaatatct cggtcgcgca
      181 tgcgcgaatt gccgaagcgg gcgccaccgc ggaagcggcg gaagcccggt tcgatggcgc
      241 cgttctcgcc gcgctccaag aaacggagac cgcgctcgac gcctatgtcc gcgagatcga
      301 ccacgaccac gcgctgaaag aggcgcgcgg cagcgcggag aaagcctccg atcaggcgcg
      361 cgcgctgttt cgcttcggcc gcaccggcat tctcgacgtg ctcaatgccg aggcggcgct
      421 cgccaccgcc gagacggcgc tcgccgcttc cgatgccgcg atcgccgatt ccgaagtcaa
      481 cgtcttccta gccctcggcg gcggctggga gccctgagaa ccaaacgaag gaacgctccg
      541 cagcgcagcc cgatccttca tcgtcaggga atggtgtttt tagcagagtc cggggagcgc
      601 tgctcagtgc tgacgagtta tagaaatcgt cagtcgtgtc gggacgaagc atgacgtgca
      661 gatcgcagac ttacgagcgg atcgtgctca aggcgtccga gctcttgcgg cgctttgggc
      721 tgcgcaagac ggcggtggct gacatcgccc atgagctcgg catgtcgccc gccaatatct
      781 accggttctt cccgtccaag cgcgcgctcg tcgaagcggt cgccgagcgg cgcctcatgg
      841 tgctgcgtca ggatctggcc ggcgtggtcc ggtcgagaaa gaccgccttc gcgcggctcg
      901 aagacctggt tcgcgcggtc gccagttctt tccgaacgat gtgcgcccag agagaccttc
      961 tcgagatcga gctcgcccgg gatctcttcg agatcgaagg gatgcgccgc gagagccaat
     1021 ggcagttcgt cgccgacttt cacgattttc tgcgtgccga gttgacgaag ctgatccggg
     1081 ccggcgtcgc ggccggcgag atgcgggtcg ccgacccggc cgacacggcg gcggcggtct
     1141 tcgacggctt catctgggtg atcgagccgc tgatgctgtt gcgggatccc gagcctgtcg
     1201 cggagcagcg actggaacga cagttccggc tgctcgcgcg cgcgctcggc gttagcaccc
     1261 ttgcgggaga ggtgacgaat gtcgcggaat gacagggatc ggcgtaaccc ttcttgggcg
     1321 gcgctgctga tttgcgtggc gcttctgccg ctcgtcctct atggctgcag ccgaaagccc
     1381 gccgcccagg cggcggcgcc gccgccccag gtgggcgtcg tgaccctcca gccggagccg
     1441 gtgaaccgca ccaccgagct gccgggccgc acctcggcct ttctgacctc ggatgtgcgg
     1501 cctcaggtgg gcggcgtcgt cctgaagcgc ctctttaccg agggcagcga cgtccaggag
     1561 ggccagcagc tttatcagat cgacccggcg ccctatcagg ccacgtacga aagcgcggtg
     1621 gcgacgctcg cccatgccga ggcggcgctc gccgacgcca acgccaagac ggcgcgctac
     1681 aagccgctgg tcgaggcgaa ggccgtcagc aagcaggact atgacgacgc cgtggcgagc
     1741 gcgcgcgagg ccgaggcgga catcaaaagt gcccacgccg ccatcgatca ggcgcgcatc
     1801 aatctcgact acaccaaggt gctcgcgccg atcgccggaa ccatcggccg ctccatcgtc
     1861 acgccgggcg cgctggtgac ggcgagtcag gccctggtgc tcgccaccgt gacgcagctc
     1921 gacccgatct atgtcgatgt gaaccagccg gtcgcgaccc tgctgcgctt cgagcgggag
     1981 ctcgcggcgg gcgagatcga gagggccgat gacggcgccg ccaaggtcac gctggagctc
     2041 gaggacggca gcgcctaccc gctgccgggc aagctcgagt tccacgaagt caacgtcaat
     2101 caaagcaccg ccacggtggt ggtgcgggca ctcttcccga acccgaagca tctgctgctg
     2161 cccggcatgt atgtgcatgc cgagatcgag gagggcgtca aggccaacgg catcctcgtg
     2221 ccgcaagcgg ccgtctcgcg caacccccgg ggcgacggca tcgtgatggt ggtcggcgag
     2281 gacggcaagg cgtcgtcgcg cgtcgtcgcg accgggccgg cggtgggcga ccgctgggtg
     2341 gtggcgagcg ggctcaatcc cggcgagcgc gtgatcgtcg acggcctgca gaaagtgcgc
     2401 cccgggatgc cggtgcagcc ggtcgcggcc ggctccagcg tcgcagaagc acccaattcc
     2461 cggacctccg gcagcgggag ctcgagatag ccatgtcgcg ctttttcatc gatcggccga
     2521 tcttcgcctg ggtgctgggc ctggtgctga tgctcgccgg cgccatcgag atcttcctgc
     2581 ttccggtggc gcagttcccc agcatcgccg cgccgcaagt gtcgatcacc gtcgtctatc
     2641 cgggcgcctc ggcgcagacg gtgcaggaca cggtcatcca gcccatcctg cagcagatgt
     2701 ccgggctcga cgggctcgaa tacatcgcct cgacggcgcg ggccgacggc tcgacggaga
     2761 tcgacttcac cttcgtccag ggcaccgatc ccaacatcgc ccaggtccag gtgcagaaca
     2821 acctgtcgct cgcggaggcg ttgctgccgg ccgtcgtcac gcagcagggg atcaaggtca
     2881 agaagtcggt caagaacttc atgctcgtcg tcgcgctggt ctccaccgat ggcagcatgt
     2941 cgggccccga catcagcgac tacatcgcct cccacctcca ggatccgctg acccgcatcc
     3001 ccggcgtcgg cgacttcacg ctgttcgggt ccgaatacgc catgcgcatc tggctcgatc
     3061 cggacaagct ctacaaatat tcgatgacgg tggtcgacgt ggccaacgcc attgccgcgc
     3121 agaacgtgca ggtctcctcg ggcgagctgg gcggcctccc ggcgagcaag ggccagcgcc
     3181 tcgatgccac catcatcggc cctgcccgct tcgagactcc ggagcagttc gccaacatcc
     3241 tgctcagggt caaccaggat ggctcgcagg tgcggctgcg cgacgtggcc cgcgtcgaga
     3301 tcggggcgca gcaatatacg ccgacggccc tcctcaatgg cgagcccacc agcgcgctcg
     3361 cgctcaagct ctcccccggc gccaaccagc tcaccaccga ggccgcggtc aaggccgagc
     3421 tcggccgcct cgcgcaattc tttccgccgg gcatcgagat cgtctatccg ctcgacaccg
     3481 agcccttcat cacgctgtcg ctgaccgaag tggcgaagac gctgctcgaa gcgatcgcgc
     3541 tcgtcttcgt ggtgatcttc gtctttttgc aggatttccg cgccacgctc attcccacca
     3601 tcgccgtgcc gatcgtgctg ctcggcacct tcgccgttct ggcgggcttc ggctactcga
     3661 tcaacacgct gaccatgctc gccatggttc tggcggtcgg cctgctggtc gacgacgcga
     3721 tcgtcgtcgt cgagaatgtc gagcgcgtga tggccgagcg cgggctctcg ccgcgggagg
     3781 cggcgcgcca gtcgatggac gagatctcgg gtgcgctcat cggcattgcc ctcgtgctgt
     3841 cggcggtgtt cctgccgatg gccttcttca gcggctcgac cggcgtcatc tatcgccagt
     3901 tctcggtgac catcatctcc gccatgacgc tttcggtgct ggtggcgctc attctcacgc
     3961 cggcgctctg cgccaccctc ttgaagcaca agccccaggg cgaagcgggc ggcgcaaagc
     4021 gcctcttcgg ctggttcaac cgcggcttcg actggaccaa ccggcgctac ggcggcggcg
     4081 tctcccgcat gatcgcgcat tcgcgctggt cgctcatcgc cttcgccgcc ctcaccgtcc
     4141 tcgccgttct cctcttcgcc cgcatgccga ccgggttcct gccggacgag gaccagggac
     4201 tgctcttcgg ccaggtcacg ctgccgccgg gtgccacggc ggaggagacc acggcggtga
     4261 acaaggaggt cgccgactat ctcctgtctc aggagaagga cagcgtcgag gacgtgctct
     4321 cggtcaccgg cttcaacttc gcgggccagg cccagaacgc cggttttctc gtcatcaagc
     4381 tcaggccctg gcgagagcgg ccgaagccct cggagtgggc gggagcggtt gcgggccgga
     4441 cgacccggcg tttcttcggc gcccgcgacg cacagatctt cgtcttcaac ttgccggccg
     4501 tgctggagct cggcaacgcc accggcttcg atctcgagct tgaggatcgc gcccagctcg
     4561 gccatgagaa gctgctcgcc gcccgcaatc agctcctcgg catggcggcg caggatcata
     4621 gtctcttcgc cgtgcgcccc aacggcctcg aggatgcgcc gcaattccat ctcgacatcg
     4681 accgcgaaaa ggccgacgcc ctgggcgtct cgatcagcga cctcgacgcc acggtccaag
     4741 gcgcgctcgg ctcgcaattc gtcaacctct tcacgcgcaa cgggcgggtg aagcaggtct
     4801 atgtccaggg cgagcccgat tcgcgcatgc tgccctcgga gctgtcgcgt tggtatgtgc
     4861 gcaacggcac cggcgacatg gtgccgttcg acgccgttct caccggctcc tggacgctcg
     4921 ggccccagaa ggtcgagggc tataacgagg tgccctcctt cgagatccag gggcagccgg
     4981 cgcccagcgg cagcaccggc gcggccatgg cgacactcga gcggatgatc cagaagctgc
     5041 cgccgggcgt cggctacgaa tggaccggcc tctcctacga ggagcagaaa gccggatctc
     5101 agaccggccc gctctatgcc atctcgctga cggtggtgct gctctgcctc gcggcgctct
     5161 atgagagctg gccagtgccg atctccgtgc tgctcgtggt gccgctgggt gtcgtcggcg
     5221 cgatctgcgc gacgctcttc cgcggcctcg acaatgacgt ctatttccag gtcgggctgc
     5281 tcaccactgt cggccttgcg accaagaacg ccatcctcat cgtcgaattc gcgaaagcct
     5341 tcttcgattc tggcatgccc ttggtcgaag cggcgaagca ggcggcgcat gagcggctcc
     5401 ggccgatcct catgacctcg atcgctttcg tctgcggcac cttgccgctc accatcgcca
     5461 ccggcgccgg cgcggcgagc cggatcgcca tcggcaccgc cgtggtcggc ggcatgctga
     5521 gcgcgacggt gctggccatc ttcttcgtcc cggtcttctt tgtcgccatt ctcagcctct
     5581 tccgcgtcaa gccgcggccg cggccgtcgg tggcgcagaa ggcgcccgtc gccgggcagg
     5641 aggcctgagc catgcgccgc tctcgcctga cgatcgcgct cctcgccggt ctcgccggct
     5701 gcagcctgat ccccgactat caccgccccg cgctcccggt ctcggagcaa tggccgggcg
     5761 gcccggccgc cgaagaggcc gcgcgcgccg tgccggccgg cgcacctccc gccgcggcgc
     5821 tgggctggcg cgacttcttc gccgatccgg cgatgcagga cctgatcgcc ttgacgctcg
     5881 agaacaaccg cgacctccgc gtcgccgcgc tcaacgtcat cgcggccgag gcgcaatacc
     5941 gggtcgatcg cgccagtctc ttcccgcagg tcgatgccac cgcggccctg cagcggagcc
     6001 gcacgccggc gagcgtcgaa ggccttgccg gcattaccgg aatctccgcg ctcaacgtcc
     6061 gcgaatacag cctcgggctg agcacggtct cgtgggagct ggattttttc gggcagatcc
     6121 gcagccaggc aaagcaggcg caggagacct atgtcagcaa cgccgagacc gagatcagca
     6181 cccggatcgc gctcatcgcc gaggtcacct ccgaatacct cacctggctc gccgatcgtg
     6241 attcgctcgc ggtctccgag gatacggtca aggtccaagc ggactcgctg cagttgacgg
     6301 agcagcggtc ggcgcgcggc actgcgaccg accaggacgt agcgcaagcc gagacgacgc
     6361 tgcgcacggc agaggccagc gccgcgcaat ttacccggca ggtcgctcag gacatggacg
     6421 aactggtgct gctcgtcggc gcgccgctgc cggaagcgct ggtggtgcgg atgaacgccg
     6481 aagccggcct cggatcgcag ccggtcttcc ccgagctccc cgccggagtt ccttcggacc
     6541 tcttggagcg ccgccccgac atccgcgccg ccgagcacgc cttgctcgcc gccaacgcca
     6601 atatcggcgc ggcgcgcgcc gctttctttc cgcagatcac gctcaccggc cagtacggca
     6661 cggccagttc cgacttgcat cacctcttcg gtcgcggctc gaccttctgg gtgttcgacc
     6721 cgtccatcag cctgccgatc ttcgatgccg gacggaacca ggccaatctc gacattgcca
     6781 aggtcgagaa gcgcatcgag gtggccaatt acgagaaggc catccaatcc gccttccacg
     6841 acgtcgccga cgcgctcgcc ggccgcggca cctatatcga cgaggtcacc gccgagcagg
     6901 gactggtcga cgccgatgcc cgcttctacg atcttgcggt gatgcgcttc cgcaacggcg
     6961 tcgacaccta tttgaatgtc ctggtggcgc agaatgcgct gctgagcgcg cggctgacgc
     7021 tgatcagcct gaagctcgcc cagcagcaga acctggtcac cctctacaag gcgctcggcg
     7081 gcggctggga ggagcggtcc caggcctcgg cgtcatcaac ccctcgcccg agcggagcgg
     7141 gagagggagc gacccgacgc gcagcgtcgg gcgggtgagg gccccggtcc gtttgcgcgc
     7201 cggctctcgg cgcgtcatct cgtcgagcat ttgattgagc ggctgagtca gatccacgcg
     7261 cgtaaagcgg cggtcaaggg cggcagcttc gcgatcgccg cagcgatctc gcgcgcccgc
     7321 ccgagcaggc gctcgcggct gacgatctcg tccaccacgc ccagcgtctt ggcctcttcg
     7381 gcgctcagcc tgtgctgggc gacgacgaag cagcggccgc ggggcgagcc gatcacatgc
     7441 ggccacaacg actgaatgtc gccgccgggc acgatgccga cgccgaaatg cggcttgtcc
     7501 tggaacac
//
LOCUS       DASYNS010000032         7221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3284980, whole genome shotgun sequence.
ACCESSION   DASYNS010000032 DASYNS010000000
VERSION     DASYNS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3284980"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..486)
                     /locus_tag="VGP50_00985"
     CDS             complement(103..486)
                     /locus_tag="VGP50_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEV8025967.1"
                     /translation="MAKTEWGLKRVCPSCGARYYDMHHDPVTCPKCGAAYDPETVAKT
                     RRARPVPVPEEPVEPLADEEIDADVATEEVEGEDEAETPAVEGGEESEEEAEEEEVIE
                     DASELGEDEDDMAEVIENVEDEEER"
     gene            659..1981
                     /gene="aroA"
                     /locus_tag="VGP50_00990"
     CDS             659..1981
                     /gene="aroA"
                     /locus_tag="VGP50_00990"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV8025968.1"
                     /translation="MTPLISRAAGPLNGRVRVPGDKSISHRALILAGVAAGRSHIRGL
                     LEGEDVLRTAAAMRAMGVALDRGADGVWRVDGVGIGGLAEPEDVLDFGNSGTGARLLM
                     GLLAAHPITAFLTGDASLRRRPMARVAEPLTRIGARIIAREGGRLPLAILGATAPLPI
                     AYQLPVPSAQVKSAVLLAGLGAPGETSVIETLPTRDHSERMLRHFGAVVTVEHEADGR
                     RITVRGQPELRAADLVVPGDPSSAAFPLVAALLMQGSQVVIESVGVNPLRTGLLDTLR
                     EMGADITILHERSEAGEPVADLAVRASMLSGVEVPAERAPRMIDEYPILAVAAAFARG
                     RTVMHGLSELRVKESDRLSAIAQGLAACGAKLAVAGDTLAVEGSAGPPPGGALIATRL
                     DHRIAMAFLVLGMAARAAVRIDDGETINTSFPGFVELMNGLGARIAPP"
     gene            1978..2634
                     /gene="cmk"
                     /locus_tag="VGP50_00995"
     CDS             1978..2634
                     /gene="cmk"
                     /locus_tag="VGP50_00995"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV8025969.1"
                     /translation="MIIAVDGPAASGKGTLAQRLADHFHLAHLDTGGLYRAAAFHALE
                     PGGDPADPKIAQAAAERVRAADLTDPRLREERIARAASVVAAIPAVRQALLAFQRGFA
                     QHPPGGAQGAVLDGRDIGTVVCPDADAKLFVTASLDARARRRFKELQESGAEAIYSLV
                     LQDMKDRDARDSQRRTAPLVPADDAFVLDTTALDADAAFAAALDFIGRKLAGKPSRKG
                     "
     gene            2757..4481
                     /gene="rpsA"
                     /locus_tag="VGP50_01000"
     CDS             2757..4481
                     /gene="rpsA"
                     /locus_tag="VGP50_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV8025970.1"
                     /translation="MAVATQHKGEEAKESFAALLDQSLGSSAGLEGTVIKGIVLAIEN
                     DQVLIDVGLKSEGRVALKEFAPPGRPAEIKPGDTVEVYLERMEDKNGEAQISREKARR
                     EEAWTQLEKSFQANERVTGVIFNRVKGGFTVDLNGAVAFLPGSQVDIRPVRDITPLLG
                     SPQPFQILKMDRSRGNIVVSRRAVLEESRAEARSELVASLKEGQILQGVVKNITDYGA
                     FVDLGGVDGLLHVTDIAWRRINHPSEALHIGQTVKVQVIRFNPETQRISLGMKQLEAD
                     PWEGVELKYPAAARFKGRVTNITDYGAFVELEPGVEGLVHVSEMSWTKKNVHPGKIVS
                     TSQEVEVQVLDVDPQKRRISLGLKQCLANPWEAFVEKYPVGTELEGEVKNITEFGLFV
                     GLPGEIDGMVHLSDLDWNKAGEEAINDYKKGDTVRVRVLDVDVEKERISLGIKQLASD
                     PFESTLAGVKKGDVVTCTVTAVSDGGVEVTLGDGLPGFIRKAELSRDRAEQRPDRFAV
                     GEKVDAKITALDRATRKVTLSIKAREFEEEKKAMAEYGSSDSGASLGDILGAALSRRG
                     DKGEKEEK"
     gene            4724..5008
                     /gene="ihfB"
                     /locus_tag="VGP50_01005"
     CDS             4724..5008
                     /gene="ihfB"
                     /locus_tag="VGP50_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEV8025971.1"
                     /translation="MTKSELIQRLAERNPHLYQRDVERIVTAIFDEIAAALSRGDRVE
                     LRGFGAFSVKRRDARLGRNPRTGDSVHVEQKHVPFFKTGKQLRERLNEEM"
     gene            5051..5377
                     /locus_tag="VGP50_01010"
     CDS             5051..5377
                     /locus_tag="VGP50_01010"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEV8025972.1"
                     /translation="MRFIYWFVALLAGIVAVDFAISNLDPAVVRFWPLGSVDTWLCIV
                     VLLALLIGFLAGEFVAWVNGRGWRREARKRARRIEALERELAATRVPSPAPELTHSPV
                     PTAPRD"
     gene            5410..6351
                     /locus_tag="VGP50_01015"
     CDS             5410..6351
                     /locus_tag="VGP50_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEV8025973.1"
                     /translation="MRMLGYSEVVAALDFPALVESLRQIFRAGADAPQRHHYTIPVPG
                     KADATLLLMPAWQANRHIGVKVVAVFPDNDADLPRLMGSFLLLDGESGKPLAVMDGPA
                     LTARRTAAASALAASYLARPDCERLLMIGTGALASHLIEAHASVRPIVNVLVWGRHYD
                     KAVKLAQRLDRRSLRVAATTDLANAVRGAHIVCCATLSETPLVEGHWLPLGVHLDLVG
                     GFRPQMREADDDCIRRGRVFVDTRAGALAEAGDIVQPLKAGILHENDIAGDLFDLARG
                     ARAGRRYHDQITIFKSVGTAIEDLAAARLAFDQVVNG"
     gene            6348..6989
                     /locus_tag="VGP50_01020"
     CDS             6348..6989
                     /locus_tag="VGP50_01020"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV8025974.1"
                     /translation="MSVAAKICGLSTPDAVAAAARHGARFVGFVFYPPSRRHVTPELA
                     AALCAIVPAGIVRTGLFVDADDDLFAEAVAKAPLDLLQLHGRESPERVAEIRRRFGKP
                     VMKAILVAAEADLAMAERYYGVADRLLFDAKPPKGAPVPGGNGLVFDWELLGGRRWAL
                     PWMLSGGLNASNLGQAVRLTHATAVDVSSGVESAPGIKDPDRIAAFLARANSL"
BASE COUNT         1119 a         2523 c         2360 g         1219 t
ORIGIN      
        1 aactgagcta tgaccccaga aggtcgtcaa atctagcgcg atgctgcgcc ccgcgggagc
       61 gccaaattag cgagcctgcc gatccccagg caagcggatt gcttagcgct cttcctcgtc
      121 ctccacgttc tcgatcacct cggccatgtc gtcctcgtcc tcgccgagct cagaggcgtc
      181 ctcgatgacc tcctcttctt ccgcttcttc ttcgctctct tcgccgcctt cgacggccgg
      241 cgtctcagcc tcgtcctccc cttcgacctc ctcggtcgcg acgtccgcgt cgatctcctc
      301 gtccgccagc ggctcgacgg gctcctcggg cactggcacc ggacgcgccc ggcgcgtctt
      361 cgccaccgtc tcgggatcat aggccgcgcc gcatttggga caggtcacgg ggtcgtgatg
      421 catatcgtag tagcgcgcgc cgcaactcgg gcagacacgc ttcaatcccc attccgtctt
      481 ggccaagcta gcctccgttc tccaatcggt cctggataaa gcgcggtgag gcggccaatt
      541 gccatgatcg cctcgccgct gtcaaaagca aaatcatcgg ggcagcgcga cggtgccccc
      601 atttgtgcgg cggcggcgtt gtgctagaga cagcgagccg cgcagcaacg ggaccgtcgt
      661 gacacccctt atttcgcgag ccgccggacc gctcaacggg cgcgttcgcg tgccaggcga
      721 caagtccatc tcgcatcgtg ctttgatcct cgcgggcgtc gccgccggcc gcagccacat
      781 ccgcgggttg ctcgaaggcg aggacgtttt gcgcacggct gccgcgatgc gcgccatggg
      841 cgtcgccctc gatcgcggcg ccgacggcgt ctggcgcgtc gacggagtgg gcatcggcgg
      901 gcttgcggag cctgaagacg tcctcgactt cggcaattcc ggcaccggcg cgcgtctcct
      961 catggggctg ctcgctgccc atccgatcac cgccttcctg accggcgatg cctccttgcg
     1021 ccggcggccg atggcgcgcg tggccgagcc gttgacgcgt atcggcgcac gcattatcgc
     1081 ccgggagggc ggacgcctgc cgcttgccat cctgggcgcc accgcgccgc ttcccatcgc
     1141 ctaccaactt cccgtgccct cggcccaagt caaatccgcc gtgctgctcg ccggcctcgg
     1201 cgctcccggc gagaccagcg tgatcgagac cctaccgacg cgcgaccaca gcgagcggat
     1261 gctgcggcat ttcggcgccg tggtgacggt cgagcacgag gccgacggcc gccgcatcac
     1321 cgtccgcggc cagccggagc tgcgtgccgc cgatctggtg gtccctggcg acccgtcctc
     1381 cgcggccttt ccgctcgtcg cggctctctt gatgcagggc tcccaagtgg tgatcgagag
     1441 cgtcggcgtc aacccgttgc gcaccgggct cctggacacg ctgcgcgaga tgggcgccga
     1501 catcacgatc ctgcacgagc gcagcgaggc cggcgagccg gtggcagacc tggccgtgcg
     1561 ggccagcatg ctgtccggcg tcgaagtgcc ggccgagcgc gccccgcgca tgatcgatga
     1621 atatcccatc ctcgccgtcg ccgccgcttt tgcccgcggc cgcacggtga tgcacggctt
     1681 gagcgagctc cgcgtcaagg aaagcgatcg cctctccgcg attgcccagg gcctcgccgc
     1741 ttgcggcgcc aagcttgccg tggcgggcga tacgctcgcg gtcgagggaa gcgccgggcc
     1801 gccgccgggc ggcgccctga tcgcgacacg gctcgatcat cgtattgcca tggctttcct
     1861 cgttctcggc atggcggctc gggcggcggt gcgcatcgac gacggcgaga cgatcaacac
     1921 cagcttcccg ggcttcgtcg agctgatgaa cggcctcggc gccaggatcg cgccgccgtg
     1981 atcatcgccg tcgatggacc ggccgcttcc ggcaaaggga cgctcgccca gcgcctcgcc
     2041 gaccacttcc atctggctca tctcgatacc ggcggccttt atcgcgccgc cgcgtttcac
     2101 gccctcgaac ccggcggcga tccggccgac cccaagatcg cgcaggccgc cgcggagcgc
     2161 gtccgtgccg ccgatctcac cgatccccgg ctgcgcgagg agcggatcgc tcgtgccgcc
     2221 tccgtggtcg ctgccatccc cgccgtgcgc caggcgctcc tcgctttcca acgcggcttc
     2281 gcgcagcatc cgccgggcgg cgcgcaggga gccgtgctcg acggccgcga catcggcacg
     2341 gtcgtctgcc ccgacgccga cgccaagctc ttcgttaccg ccagcctcga cgcccgtgcc
     2401 cggcggcgct tcaaggagtt gcaggaaagc ggcgccgagg ctatatacag cctcgtcctg
     2461 caggacatga aagaccgtga cgcgcgcgac agccagcgtc ggacggcgcc actggtgccg
     2521 gccgacgatg ccttcgttct cgacacgacc gcgctcgatg ccgatgccgc cttcgccgcg
     2581 gcgctcgact tcatcggccg gaagctcgcc ggcaaacctt cccgcaaggg gtgatgagcc
     2641 gttttcgcct ggcaggatac gggcattaac cgccgcgccc gcgcttgtcg gcggctgtcg
     2701 cgttcgcttc atccccagcg gcgcgccggc tcatcccggg gagatttgga gtacggatgg
     2761 ctgtcgctac acaacacaag ggagaggaag ccaaggagag cttcgcagcg ctgctcgatc
     2821 aatcgctcgg cagcagcgct ggactcgagg ggacggtcat caaaggcatc gtcctcgcca
     2881 tcgagaacga tcaagtgctg atcgatgtcg ggctcaaatc cgaagggcgc gtggcgctca
     2941 aggaattcgc cccgcctggg cggccggcgg agatcaagcc cggcgacacc gtcgaggtct
     3001 acctcgagcg gatggaggac aagaacggcg aggcgcaaat ctcgcgcgag aaggcgcggc
     3061 gagaagaagc ctggacgcag ctcgagaaga gcttccaggc gaatgagcgc gtgaccggcg
     3121 tcatcttcaa ccgcgtcaag ggcggcttca ccgtcgacct gaacggcgcc gtggctttcc
     3181 tgcccggaag ccaggtggat atccgtcccg tgcgcgacat cacgccgctc ttgggctcgc
     3241 cgcagccttt ccagatcctc aagatggatc gctcgcgcgg caacatcgtc gtctcgcgtc
     3301 gcgctgtcct cgaagagagc cgcgccgagg cacgctcgga gttggtcgcg agcctcaagg
     3361 aaggccagat cctccagggt gtcgtcaaga acatcaccga ttacggcgcc ttcgtcgatc
     3421 tgggcggcgt cgacggcctg ctccatgtga cggacatcgc ttggcggcgc atcaatcatc
     3481 cttcggaagc gctgcatatc ggccagaccg tcaaggtcca ggttatccgc ttcaatcctg
     3541 agacgcagcg catctcgctc ggcatgaagc agctcgaggc cgatccctgg gagggcgtgg
     3601 agctcaaata cccggcggcc gcgcgcttca agggccgcgt caccaacatc acggattatg
     3661 gcgctttcgt cgagctggag cccggagtcg agggcctcgt ccatgtctcg gagatgagct
     3721 ggaccaagaa gaacgtgcat cccggcaaga tcgtctcgac gagccaggag gtcgaggtgc
     3781 aggtgctcga tgtcgatccg cagaagcggc gcatcagcct cggcttgaag cagtgcctgg
     3841 ccaatccctg ggaagctttc gtcgagaagt atccggtcgg caccgagctc gaaggcgagg
     3901 tcaagaacat caccgagttc ggtctcttcg tcggtctccc cggcgagatc gacggcatgg
     3961 tgcatctctc ggacctcgac tggaacaagg cgggcgaaga ggcgatcaac gactacaaga
     4021 agggcgacac cgtccgggtg cgcgttctcg acgtcgacgt ggagaaggag cgcatcagcc
     4081 tcggcatcaa gcagctcgcg agcgatcctt tcgaatcgac cctcgccgga gtcaagaagg
     4141 gcgacgtcgt cacctgcacg gtgaccgccg tcagcgacgg cggggtcgaa gtgacgctcg
     4201 gcgacggcct gccgggcttc atccgcaagg cggagctgtc gcgcgaccgc gccgagcagc
     4261 gcccggaccg tttcgccgtc ggcgagaagg tcgatgccaa gatcaccgcc ctcgaccgcg
     4321 ccacgcgcaa ggtgacgctg tcgatcaagg cgcgcgaatt cgaggaagag aagaaggcca
     4381 tggccgagta cggctcctcg gattcaggcg cgagcctcgg cgacattctc ggcgccgcct
     4441 tgagccgccg cggcgacaag ggcgaaaaag aggagaaata ggaggcaggg cgtcgccgag
     4501 cctctccctc tccctttcgg ggagaggggt ctgggcgagc gggggcgggt ctgccgcccg
     4561 ctccttcctc ttaccttctc gcggccggca ttggctggac cgttctgatc gccgggccgg
     4621 cgccgcgccc cggtcgctgg atctgctatc cgtgatttct tgacgctggc tcaagggttt
     4681 ggcaggcttt gccttttccg gggagcccga tgacacgatg gctatgacca agtccgagct
     4741 catccagcgc ttggccgaac gcaacccgca tctctatcag cgtgacgtcg agcgcattgt
     4801 caccgccatc ttcgacgaga tcgccgccgc gctctcgcgc ggcgaccgcg tcgagctccg
     4861 cggcttcggc gccttctcgg tgaagcgtcg cgacgcgcgc ctcggacgga acccgcgcac
     4921 cggcgacagc gtgcatgtcg agcagaagca cgttcccttc ttcaagaccg gcaagcagct
     4981 gcgtgagcgc ctcaatgagg agatgtgagc gccgcggcgc tgcgcccgcc gaactcgggc
     5041 aggcggcgaa gtgaggttca tctactggtt cgtggcactg ctggcgggca tcgtcgccgt
     5101 cgatttcgcg atctccaatc tcgaccccgc ggtggtccgt ttttggccgc tggggagtgt
     5161 cgacacgtgg ctctgcatcg tcgtgcttct cgccttgctc atcggctttt tggctggcga
     5221 gttcgtggcc tgggtcaacg gacgcggctg gcggcgcgag gcgcgcaagc gcgcccggcg
     5281 catcgaagcg ctggagcgtg agctcgccgc gacccgagta ccgtcgcccg cgccggagct
     5341 cacgcattct cccgtgccca cggcgccgcg cgattgactc gcacccgggc cgcaggatac
     5401 tccctcgcca tgcggatgct cgggtactcg gaagtcgtcg ccgcgctcga ctttcccgct
     5461 ctcgtcgagt ctttgcggca gattttccgc gccggcgccg atgcgccgca acggcatcac
     5521 tacacgatcc cggtgcccgg caaggctgac gcgacgcttc tcctcatgcc cgcgtggcaa
     5581 gcaaaccgcc acatcggcgt caaggtggtc gcggtgttcc cggacaacga tgccgacctg
     5641 ccgcggctga tgggatcctt cctcctgctc gacggcgaga gcggcaagcc gctcgcggtg
     5701 atggacgggc cggcgctcac ggcacggcgc accgctgcgg catcggcgct cgcggcgagc
     5761 tatctggcac gccccgattg cgagcggctg ctcatgatcg ggaccggcgc gctcgcgtca
     5821 catctcatcg aagcgcatgc gagcgttcgg ccgatcgtca atgtgctggt ctggggccgc
     5881 cattacgaca aggccgtgaa actcgcgcaa cggctcgatc ggcgcagcct cagggtcgcc
     5941 gccaccaccg atctcgccaa tgcggtgcgc ggcgcgcata tcgtctgctg cgcgaccttg
     6001 agtgaaactc cgctcgtcga aggacattgg ctgccgctcg gcgtgcatct cgatctggtc
     6061 ggcggcttcc gtccgcaaat gcgcgaggcc gacgacgatt gcatccggcg cggccgcgtc
     6121 ttcgtcgaca cgcgcgccgg cgcgctggcc gaagcgggcg acatcgttca gccgctcaaa
     6181 gcgggcattt tgcacgagaa cgacatcgcc ggcgatctct tcgacctcgc gcgcggcgcc
     6241 cgtgcgggcc ggcgctacca cgatcagatc accatcttca aatccgtcgg caccgcgatc
     6301 gaggatctgg ctgcggcccg cctcgcattc gaccaggtgg tcaacggatg agtgtcgctg
     6361 ccaagatctg cgggttgtcg acgccggacg cggtggccgc ggcagcgcgc catggagcgc
     6421 gcttcgtggg cttcgtcttc tatccgccga gccggcgcca tgtgaccccc gaactcgccg
     6481 cggcgctctg cgccatcgtt ccggcgggca tcgtccggac cgggctcttc gtcgatgccg
     6541 atgacgatct tttcgccgaa gcggtggcca aggcgccgct cgaccttctt cagctccacg
     6601 ggcgcgaaag cccggaacgc gtcgccgaga tcagacgccg cttcggcaaa cccgtcatga
     6661 aggcgatcct ggtggccgcc gaagcagatc tcgccatggc cgagcggtat tacggcgtcg
     6721 ccgaccggct gctcttcgac gcgaagccgc ccaagggcgc gcccgtcccc ggcggcaacg
     6781 gactcgtctt cgactgggag ctgttgggcg gccggcgctg ggccttgccc tggatgctgt
     6841 cgggcggcct caatgcctcc aatctcggtc aggccgttcg gctcacgcat gcgacggcgg
     6901 tcgatgtctc ttcgggcgtc gagagcgcgc ccggcatcaa ggacccggac cggatcgccg
     6961 ccttcctggc gcgcgcaaac tcgctgtgac gccattttga acgcttggcg agttgcgcta
     7021 aaagacgccc gcggcggcga gctttcagca gatttatgaa gcggcaacac cccccccacc
     7081 ccaaccctcc ccctcaaggg gggagggggt tcgtccatgg gctgtattct tacccctccc
     7141 cccttgaggg ggagggaggg gtggggggta gggcgcccca cagcgggccg cgttagtccg
     7201 atggccgttc ccaataccta t
//
LOCUS       DASYNS010000033        15474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2349464, whole genome shotgun sequence.
ACCESSION   DASYNS010000033 DASYNS010000000
VERSION     DASYNS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 15474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15474
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2349464"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1853
                     /locus_tag="VGP50_01025"
     CDS             <1..1853
                     /locus_tag="VGP50_01025"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEV8025975.1"
                     /translation="RNAIIVDIEHAPRNPNGTVSYSVDFQILRPIDLSKGNHRIVFEP
                     PNRGTPLMFSYLNSSPSPNNKLSAGNPGNGFLMSQGYTIVEGAWDTTVPVQNPASPLF
                     GVTFPVATNPDGSPIVGPATEEFVVDFTAQPATQPLTYPAATADKSQASLTVRENYGD
                     TPQPVPSSGWDYANASLTAVKLTSGNFGGPGSFGPTALYEFTYQAKNPLVSGLGFAAV
                     RDFATFLRDARADDAGNANPVAGDVQHIFTFCVSQPCRLMRDFVWLGFNEAEAGGGVK
                     VFDGVFNWIGGGNGLFINYRFAQPFRTHRQHIARWTPEFQFPFANQVITDPVTGKTDG
                     RLARCQATGTCPKIFESNSENEYWAKGGSMLTTDGAGHDLDLDQAPNVRTYFLASHPH
                     APGTAPGICQQPQNPLIPAQVVRALLVDLDEWVSIGRLPPFDQIPRLLDGTLVPALPQ
                     ARVGFPHIPGVVYNGVLHTGDLWNFGPEFDAGILTILPPVLLGTPYQIFVPRTDFDGN
                     DLAGIRVPDVAVPLATYTGWGLRAAAPGDPVPIVDGCDASGQRIPFAATLAERLASGD
                     PRPSLQERYPSHAIYVELVTAVARDLELERLLLDADVQAYIAAAQAAAIP"
     gene            complement(1929..3152)
                     /locus_tag="VGP50_01030"
     CDS             complement(1929..3152)
                     /locus_tag="VGP50_01030"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEV8025976.1"
                     /translation="MAEPQARGLTPMREPILVINAGSSSIKFSVFETAADRSLSAGAH
                     GEVEGIGTSLHFTVADAQGRKLADQPVSGKDHAAAIAAIEAWFAAHVGGEQGFDGVGH
                     RVVHGGTAYFQPVLVDDQVIATLEALVPLAPLHQPHNIAAIRAIRAAAPRMKQVACFD
                     TAFHRDQPALAQEFALPRALTAKGVRRYGFHGLSYEYIVSALPQVAPECAQGKIVVAH
                     LGNGASMCAIEHGRSIATTMGFTAVDGLPMGTRTGVLDPGVILYLLQHERMDATAIEH
                     LIYERSGLLGVSGLSSDMRTLLASDLPAAQEAVDLFVYRIGRELGSLVAALGGLDALV
                     FTGGIGEHAPEIRGRVCQGASWLGVALDADANAAGGPRISKANSPISAWVVPTDENLM
                     IARHTRRLLDARESS"
     gene            complement(3454..4203)
                     /gene="flgH"
                     /locus_tag="VGP50_01035"
     CDS             complement(3454..4203)
                     /gene="flgH"
                     /locus_tag="VGP50_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HEV8025977.1"
                     /translation="MHLLFGTLRPLALIGLVALGGCNAITRVSEIGEQPPLTTIQDPH
                     QQPGYKPVSLPMPTPIVSERRPNSLWQAGSRAFFKDQRAAQVGDILTVVVNFDEKAQF
                     NNETQRQRTSSDTESAGSLFGLETKIPKIFTGANPSSLINTAGTSNADGKATMDREEQ
                     VNVQVAALVTQVLPNGNLVVQGHQEFRVNFEVRELQVAGVIRPQDISSVNTVDYSKLA
                     EARISYAGHGQLMDIQQPRYGQQLLDIVAPF"
     gene            complement(4296..5243)
                     /gene="flgA"
                     /locus_tag="VGP50_01040"
     CDS             complement(4296..5243)
                     /gene="flgA"
                     /locus_tag="VGP50_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HEV8025978.1"
                     /translation="MNRLFASLLLLVVALAAGRAAAEPALRPSAVVEGTMIHLGDIFA
                     DAGARANDVVAPAPPPGSRTIFNAAWLAATAREHLLDWEPASRFDQAVVERATRSIGA
                     DAIIARLRAEIGERRPGEDLEIELDDRAWHLLVPAEAADAIAVDELSIDPRSFRFSAL
                     VTTAAGSAEAAPKRVAGRLIRMIALPVLDHAMMPGDIIGPHDIATARLAADRVAADAL
                     ADAREFLGKTPRRPLRAQEPLRLGDVQAPLLLHKGDIVTIILETPTMRLTAEGRALED
                     GAMGAPVHVANTKSGRVLDARVTGDGIVAIDTRALVAAR"
     gene            complement(5258..6043)
                     /gene="flgG"
                     /locus_tag="VGP50_01045"
     CDS             complement(5258..6043)
                     /gene="flgG"
                     /locus_tag="VGP50_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HEV8025979.1"
                     /translation="MRAMNIAATGMSAQQTYVEVTANNIANLNTTAFKRQRPEFQDLL
                     YQNERRGGATSSDTGTIVPVGVQIGIGVKTAAVYRITTQGNLTQTSNPLDVAIQGRGF
                     FQILQPDGTTAYTRAGSFQLSPTGEIVTADGFVVQPGITLPQNTVGVTINASGQVQAQ
                     VAGQTTPQTVGQLQLANFANEAGLEALGNNLFQETPAAGSAVTGNPGAIGFGTINQGL
                     LETSNVDVVTEITNLITAQRAYEMNSKVIQTADQMQSTLNQIQ"
     gene            complement(6059..6802)
                     /gene="flgF"
                     /locus_tag="VGP50_01050"
     CDS             complement(6059..6802)
                     /gene="flgF"
                     /locus_tag="VGP50_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEV8025980.1"
                     /translation="MQNATSISLSSQTALQRQMDVVANNLANLSTPGYKAEEMLFTQH
                     LTHTRGTGTIAFVEDAGTARDLRQGPISKTGNPLDFALQGPGYFSVETPLGTRYTRAG
                     HFQLDAQHQLVTSEGYPVLAAGGQPLALPSNINAVTVGTDGTVSASQAGSSSQSSVGK
                     LQVVTFAAPQLVTPAANGLYVTDQTPDPATTATTTVQQGMIEESNVQPVVELTRLMAV
                     SRNSGAIKNVSDEESQRQGNAYDKLSKVY"
     gene            7135..7662
                     /locus_tag="VGP50_01055"
     CDS             7135..7662
                     /locus_tag="VGP50_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HEV8025981.1"
                     /translation="MADRALDDAAEVPAMLPEGQAPAPAGGRKKLIIIVLGAVLLLAG
                     AGGGLYFTGIVDKFLPHKAEVVDKAAAPAAPPVFFDMQDLLVNLNTVGRKQSFLKLSI
                     SLELERADDLPRLQAVMPRVIDTFQVYLRELRVDDLRGSAGIYRLREELLTRVNAAAA
                     PVKVTDVLFKEMLVQ"
     gene            7673..8770
                     /gene="fliM"
                     /locus_tag="VGP50_01060"
     CDS             7673..8770
                     /gene="fliM"
                     /locus_tag="VGP50_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HEV8025982.1"
                     /translation="MAEPKPPIDEEKLAQEWAAMASTEGVQGDKAVAEGESEAAVTAR
                     ILNQNEIDSLLGFADSDNSAKEGSGIRAIVDSALVSYERLPMLEVVFDRLVRLMSTSL
                     RNFTSDNVEVSVDNVTSIRFGDYLNSIPLPAMLSVFKAEEWDNYALLTVDSSLIYSIV
                     DVLLGGRRGAGSLRVEGRPYTTIERNLVERLVAVVLSDLSGAFEPVSPVTFRFERLET
                     NPRFATIGRPANAAVLARLRIDMEDRGGRLEVLLPYATLEPVREILLQGFLGEKFGRD
                     SIWEAHLAGELWHTSVPIEAVLDTVELPLHEVLALKVGSRIILNANINSDVGLVCGDL
                     PMFSGKMGRRGGNIAVRITEKTEKKDKGRTP"
     gene            8767..9204
                     /locus_tag="VGP50_01065"
     CDS             8767..9204
                     /locus_tag="VGP50_01065"
                     /inference="COORDINATES: protein motif:HMM:NF039465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6468 domain-containing protein"
                     /protein_id="HEV8025983.1"
                     /translation="MTLALAGDLIVAVLLLVTIVYSTVLNRRLAALRGDKDELHALVE
                     GLAKTTASAGAGIAALKAAAETVGRELEAGRAKAQALRDDLAYLVERGNSAADRLEGG
                     IRAQRQAVRAAETPAAAAPTPIAGASASRAERDLMRALAGQRQ"
     gene            9290..9964
                     /locus_tag="VGP50_01070"
     CDS             9290..9964
                     /locus_tag="VGP50_01070"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025984.1"
                     /translation="MAYRVRVMPLAILASIGLLGIKLGDLSSLVLTTSIARAEGATPA
                     APAPAAATPAPAATDAHSPPSAPPAATDIDPLSMSPSEIDLLQKLAERRTELDKRAAD
                     LSQREILLQAGEKRVDEKIVKLAALEKDIADIVEKQNDETEQRTKSLVKIYETMSPKD
                     AARIFEQLDMGVLLAVVEHMKERNAAPILAAMDAAKARTVTQALAERSDRHPKVDGST
                     KTAAKP"
     gene            10047..10697
                     /locus_tag="VGP50_01075"
     CDS             10047..10697
                     /locus_tag="VGP50_01075"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HEV8025985.1"
                     /translation="MKSADDGDLARKLEAVQSRSPSTVETSEVASIVESILESLSGDM
                     SVADLKLYRELEHLAGYIHRAKREIAELHPHEIRERHIPAATDELDAVVDATAAATGH
                     ILDEAEAIEKLKAGLPPETAILIAAAVARIYEACSFQDITGQRINKVVKTLRYIEDKI
                     DALLSAFDGGVGGEPAAAPMGPSKAPDEEFISGPQLPDNANNQADIDAILASLDKQ"
     gene            10694..13663
                     /locus_tag="VGP50_01080"
     CDS             10694..13663
                     /locus_tag="VGP50_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8025986.1"
                     /translation="MIRRSALAVALLILALTGATAGAKDAPPSRAVAASTGHLHFSEH
                     RDFRRVIFNWHEPVAYVLIGAEGVARLRFVGAAQLDAAELQAALPDLSPTLRAQGNTT
                     VVSFRLPAGVKLRHFRSGTAIVVDILRPGAPSGPRTPAPAVQALCGPSGSCPELQTAK
                     AELKTATAEPRPQPAPAPADSGKGDTDKPAAPGNPIDLAPAAQAVAATAAADALLVQG
                     AATPEGVRLRFDWPKPTAAAVFRRAGAVWIVFATPAALDLAEPRKLVGGALSTLDQIA
                     DERATVLRLVPRAGLAPSVRRDGNSWIVELKAQDAQPDTPLNVETRPAADTPSVLFAL
                     TDAAEPLRLRDPELGDSLVVVPLTAAGTGALEQSFVDFRVLASVQGLALRPNVDDLIV
                     ASTLDGLAVTRPGGLLLAGKIEHRLANLADGGLRLLDFASWRGEHDANFLRTRSRLEH
                     LVTTASPGERSRRRLALAHFYFANFFAAEAEGVLEAIRRDDPDLAADPAVALMIGAAE
                     LLTGDRRAAAQDLGRQVLDGQIEATLWRASLAAETGDWPAAARGFITTVSLLPLYPKP
                     LRDRFALEAAEALLETSQPDAVPPILDLVLGGRPSRADEAMALYLDGRRADQQGDRAH
                     AIERWTAVAGMGEPRSRVRSLIARATAQLETKEISRADAIKVLDGLRFDWRGDFVEFE
                     LLRRLGELQLAEGDRRDALDTLREAAVNFPDYPAAKAVMTELSDAVAEMFLGDAMKNL
                     SPLKALALYDEFKDFTPVGERGDRILSGLVDRLVAVDLLDRAAALLSEQIEHRLQGHD
                     KARAATQLALLRLLDHKPEAALQALAIPIDRDAPVELLRQRVQLRARALTDLQRPDEA
                     LAVIADDHSRDADRLRADIFWHGQKWGEVVTVLSRLAPTPPSDGKLDDGSTQLVLNLA
                     AAMILAGDKAGLSQLRTVYGKAMSGTPSGDAFHVLAGESAPGAKDVDPREFASHVAQL
                     SELQGFMTSLRGPPAKN"
     gene            complement(13679..15217)
                     /locus_tag="VGP50_01085"
     CDS             complement(13679..15217)
                     /locus_tag="VGP50_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator"
                     /protein_id="HEV8025987.1"
                     /translation="MRRLTRRSLSILALLPGVLALASPLRAEDKPASDTPAKPSAAAS
                     SALPHDAVTHHKIRLGGQDIAYTATAGTLPLRDEKGEKQADIFYVAFLREGVADAQPR
                     PITYAFNGGPGAAAAYLDIGALGPRALDFGPAGRPQPAVNHVVDNPDSWLAFTDLVFI
                     DPVGTGYSRAVGGSDEARKLWSVRADLDALAQIIRVHLTRVGRLTSPVYLAGESYGGF
                     RAARLAHQLGQRQGVAATGVLLISPVLEFRLMSGDEFDPIPWVLRLPAYAAVALEAKG
                     ALTPEALVDAERFALGDYLTALAAGPGDAAAAQPLYARIGALIGLDTEAVARWEGRVP
                     VSVYVKEMRHRDEQVLSRYDGGIAAPDPDPRSRDAEDDPVLEGSMAPFTQAFVAYVRT
                     ELGFETELGYELLNDEVNHHWEWRDGEHGGGRQTLGASEALRTALALNPRLRVLIAHG
                     LTDLTTPYMMSRYVVDHMPRGITGQRIDLKLYPGGHMMYLRTGSRHRLHDDAQAFYGG
                     DAAQ"
BASE COUNT         2430 a         5237 c         5342 g         2465 t
ORIGIN      
        1 cccgcaacgc gatcatcgtc gacatcgagc acgcgcccag gaatcccaac gggaccgtca
       61 gctactccgt ggatttccag atcctgcggc cgatcgatct gagcaaaggc aaccaccgca
      121 tcgtcttcga gccgcccaat cgcggcacgc cgctgatgtt ctcctacctc aacagcagcc
      181 cgtcgccgaa caacaagctg agcgccggga atcccggcaa cggcttcctg atgagtcagg
      241 gctacaccat cgtcgaaggc gcgtgggaca ccaccgtgcc ggtgcagaat ccggcgagcc
      301 cgctcttcgg cgtcaccttc cccgtcgcca cgaatcccga cggctcgccg attgtcggcc
      361 cggccaccga ggaattcgtc gtcgacttca cggcgcagcc cgcgacccag ccgctgacct
      421 atccggcggc gaccgccgac aagtcgcagg cgagcctgac cgtgcgcgag aattacggcg
      481 atacgcccca gcccgtgccg agcagcggct gggactatgc caatgccagc ctgacggccg
      541 tgaagctgac ctcgggcaat ttcggcgggc caggctcctt cgggccgacc gcgctctacg
      601 aattcaccta tcaggccaag aacccgctgg tgtccgggct gggctttgcc gccgtgcgcg
      661 acttcgccac cttcctgcgc gatgccaggg ccgacgatgc cggtaatgcc aatccggtcg
      721 ccggcgatgt gcagcacata ttcacctttt gcgtgtcgca gccctgccgc ctcatgcgcg
      781 acttcgtgtg gctgggcttc aatgaggccg aggcgggcgg tggcgtaaag gtcttcgacg
      841 gcgtcttcaa ctggatcggc ggtggcaacg ggctcttcat caattaccga ttcgcccagc
      901 ccttccgcac ccatcgccag cacatcgccc gctggacgcc cgagttccag ttccccttcg
      961 ccaaccaggt catcaccgac ccggtgaccg gcaagacgga cgggcgcctc gcgcgctgcc
     1021 aggcgaccgg gacctgcccc aagatcttcg agagcaattc ggagaacgag tattgggcga
     1081 agggcggctc gatgctcacc accgatggcg cggggcatga tctcgacctc gaccaggcac
     1141 ccaatgtccg gacctatttc ctcgcgagcc acccgcacgc gccgggcacc gccccgggca
     1201 tctgccagca gccgcagaac ccgctgattc ccgcccaagt cgtgcgcgcg ctgctggtcg
     1261 atctcgacga atgggtcagc atcggccgcc tgccgccctt cgatcaaatc ccacgcttgc
     1321 tcgacggcac gctcgtgccg gctttgccgc aggcgcgcgt cggcttcccg catattcccg
     1381 gcgtcgtcta caacggcgtc ctccacaccg gcgatctctg gaatttcggg cccgaattcg
     1441 acgcgggcat cctgacgatc ctgccgccgg tcctgctggg cacgccctat cagatcttcg
     1501 tgccgcgcac cgatttcgac ggcaacgacc tcgccggcat tcgcgtaccc gacgtggccg
     1561 tgccgctcgc gacctacacc ggctgggggc tgcgcgccgc ggcacccggc gacccggtgc
     1621 ccatcgtcga tggctgcgac gcctcgggcc agcgcattcc cttcgccgcg acgctggcgg
     1681 aacgcctggc ctctggcgat ccgcggccgt cgctccagga gcgctacccg tcgcatgcaa
     1741 tctacgtcga gctggtgacg gcggtcgcca gagacctcga gctcgagcgc ctgctgctcg
     1801 atgccgacgt gcaggcctac atcgccgcag cgcaggcggc ggcgatcccg taagcacgac
     1861 gtagcaccgg aaattccctc tcctctcgcg ggagagggaa tgaccgcgcc gttcggcgtc
     1921 gacttggctc atgacgattc cctggcgtcg agaagccggc gcgtatgtcg cgcgatcatc
     1981 aaattctcgt cggtcggcac gacccacgcc gatatcgggg agttggcctt cgagatgcgc
     2041 gggccgcccg ccgcgttcgc atccgcgtcg agtgccacgc cgagccagct cgcgccttgg
     2101 cagacccggc cccgaatctc cggtgcatgc tcgccgatgc cgccggtgaa gacgagcgcg
     2161 tcgagcccgc cgagtgcagc aacgagcgag ccaagctctc gcccgatgcg atagacgaag
     2221 aggtcgaccg cttcctgtgc ggcagggaga tcgctcgcca gcagggttcg catgtcgctc
     2281 gacaggccgg aaacgcccag caagcccgac cgttcataga tgaggtgctc gatcgccgtc
     2341 gcatccatcc gctcatgctg aagcagatag aggatgacgc cggggtcgag aacgccggtc
     2401 cgtgtcccca tcggcaggcc gtcgactgcg gtgaagccca tcgtggtggc gatgctgcgg
     2461 ccgtgctcga tcgcgcacat gctggcgccg ttgccgaggt gggcaacgac gatcttgccc
     2521 tgggcgcact ccggcgccac ttgcggcagc gccgacacga tgtattcata ggacaagccg
     2581 tgaaagccat agcggcgcac gcccttggcc gtcagcgcgc ggggcagcgc gaattcctgc
     2641 gccagtgccg gctggtcccg gtggaacgcc gtatcgaagc aggcgacctg cttcatgcgc
     2701 ggcgccgcgg cgcgaatggc gcggatggcg gcgatattgt gcggctggtg cagcggcgcc
     2761 aaaggcacca gggcctcgag cgtcgcgatg acctggtcgt cgacgagcac cggctgaaaa
     2821 tacgccgtcc cgccatggac gacgcgatgc ccgacgccat cgaagccctg ctcgccgccg
     2881 acatgcgccg cgaaccacgc ctcgatcgcg gcgatggcgg ccgcgtgatc tttgcccgaa
     2941 accggctggt cggccagctt gcgtccctgc gcatcggcga ccgtgaagtg cagcgacgtc
     3001 ccgatgccct cgacctcccc atgcgcaccg gccgagagcg agcggtcggc cgcggtttcg
     3061 aacaccgaga atttgatgct ggaggagccg gcgttgatga cgaggatcgg ctcgcgcatc
     3121 ggcgtcaatc cgcgagcttg cggctcggcc atcgcaaacc ctctatagcg acgctgtggc
     3181 ggtgcatggt cgttccggcg atctggcccg gttccgctat gtcgcgaaga tcacccttga
     3241 gcacatgagc gcgcgccgct cgccccggaa cgggtcgtat ctcgtcgaag cggagagaga
     3301 gtgacgatct ttgccgcgac gtgccttgat gtagatcaag acgtcaggat ggtcaacgca
     3361 gctaattccc cgaggtgcgc tcccgcggtt tgctttcgca aaccgctgac gccgcccccg
     3421 acgaacgcgc ttcgcgtgtt cgcggggcgt tcgtcagaac ggggcgacga tgtcgaggag
     3481 ctgctggccg tagcgcggct gctggatgtc catgagctgg ccgtggccgg cataggagat
     3541 gcgcgcctcg gcgagcttgc tgtagtcgac ggtgttgacc gagctgatgt cctgcggacg
     3601 gatcacgccg gcgacttgga gctcgcgcac ctcgaaattg acgcggaact cctggtgtcc
     3661 ttggacgacg aggttgccat tgggcagcac ttgggtcacg agcgctgcca cctgcacatt
     3721 gacctgctcc tcgcggtcca tggtggcctt gccgtcggcg ttgctggtcc cggcggtgtt
     3781 gatgaggctg ctcgggttgg cgccggtgaa aatcttcggg atcttcgtct cgagaccgaa
     3841 gaggctgccc gcggattccg tgtcggagga ggtgcgctgg cgctgcgtct cgttgttgaa
     3901 ctgcgctttc tcgtcgaaat tgaccacgac ggtgaggatg tcgccgacct gagccgcgcg
     3961 ctgatccttg aagaaggcgc ggctgccggc ctgccacagc gagttgggcc ggcgctcgct
     4021 gacaatcggt gtcggcatcg gcagcgacac gggcttgtag ccgggctgct gatgcgggtc
     4081 ctgaatggtg gtgagcggcg gctgctcgcc gatctcagag acccgcgtga tcgcgttgca
     4141 gccgccaagc gcgacgaggc cgatcaaggc gagcgggcgg agcgtgccga acaaaaggtg
     4201 catgacgcct ccatgagcgc gacacgaatc cagtccagag ctgtcgcgag gacgacgata
     4261 ttgagtggga aagaatttca ttgcgccgga ccccgtcaac gcgcggcgac gagagcgcgg
     4321 gtgtcgatcg caacaatgcc gtcgccggtg acgcgcgcgt cgaggacgcg gcccgacttg
     4381 gtgttggcga catggacggg cgcgcccatc gcgccgtcct cgagcgcacg cccttcggcg
     4441 gtgaggcgca tcgtcggcgt ctcgagaatg atggtgacga tatcgccctt gtgcagcagg
     4501 agcggcgcct ggacatcgcc gaggcgcagc ggctcctgcg cgcggagcgg ccggcgcggc
     4561 gtcttgccca ggaactcgcg cgcatccgcc agcgcatcgg cggcgacgcg gtcggcggcg
     4621 agccgcgcgg tggcgatgtc atgcgggccg atgatgtcgc ccggcatcat ggcgtggtcg
     4681 agcacgggga gcgcgatcat gcggatgagc cggccggcga cgcgttttgg cgccgcctcg
     4741 gcgctgcccg cggccgttgt gacgagcgcc gagaagcgga agctgcgcgg atcgatcgag
     4801 agttcgtcga cggcgatcgc atcggcggcc tcggcgggaa cgaggaggtg ccacgcgcga
     4861 tcgtcgagct cgatctcgag atcctcgccc gggcggcgct cgccgatctc cgcgcgcagg
     4921 cgcgcgatga tcgcgtcggc gccgatgctg cgcgtcgcgc gctcgacgac ggcctggtcg
     4981 aagcgggaag cgggctccca gtcgagaaga tgctcgcgcg cggtcgcggc gagccaggcg
     5041 gcgttgaaga tcgtgcgcga gcccggcggc ggcgccggtg ccacgacgtc gtttgcgcgc
     5101 gcgccggcat cggcgaagat atcgccgagg tggatcatcg tgccttcgac gaccgcggag
     5161 ggccgcagcg ccggctccgc ggcggcgcgc ccggccgcga gcgcgacgac aagcagcaga
     5221 agagaggcga agaggcggtt catggagggt gccggcgcta ctgtatctgg ttcagcgtcg
     5281 actgcatctg gtcggcggtc tgaatcacct tcgagttcat ctcataggcg cgctgcgccg
     5341 tgatgaggtt ggtgatctcg gtgacgacgt cgacattcga ggtctcgagc aggccctggt
     5401 tgatcgtgcc gaagccgatc gcgccggggt ttccggtcac ggccgagcct gcggccggcg
     5461 tctcctggaa gagattgttg ccgagcgcct cgaggcccgc ctcgttggcg aaattcgcga
     5521 gctggagctg gcccaccgtc tggggcgtcg tctggccggc gacctgcgcc tgcacctggc
     5581 cgctcgcgtt gatggtgacg cccaccgtgt tctgcggcag cgtgatgccg ggctgaacga
     5641 cgaagccgtc ggcggtgacg atctcgcccg tcgggctcag ctggaacgag ccggcgcgcg
     5701 tataggcggt ggtgccgtcg ggctggagga tctggaagaa gccgcggccc tggatcgcga
     5761 cgtcgagcgg gttgctggtc tgcgtcaggt tgccttgcgt ggtgatgcgg tagaccgcgg
     5821 cggtcttgac gccgatgccg atctgcacgc cgacgggcac gatggtgccg gtgtcggaag
     5881 acgtggcgcc gccgcgccgc tcgttctgat agagcaggtc ctggaactcc gggcgctggc
     5941 gcttgaatgc cgtcgtgttc agattggcga tgttgttggc agtgacctcg acataggtct
     6001 gctgggcact catgcccgtg gcggcgatgt tcatggcgcg catggcggcg aattctcctt
     6061 agtagacctt gctgagcttg tcgtaggcat tgccttggcg ttggctttcc tcgtccgaga
     6121 cgttcttgat cgcgccgctg ttgcgcgaca ccgccatcag ccgcgtgagc tcgacgaccg
     6181 gctgcacatt cgactcctcg atcatcccct gctgcacggt ggtggtggcc gtggtcgcag
     6241 ggtcgggcgt ctgatcggtg acgtaaaggc cgttggcggc gggcgtcacg agctgcggcg
     6301 cagcgaaagt cacgacctgc agcttcccca ccgagctctg gctcgagctg ccggcctggc
     6361 tcgcggatac ggtgccgtcg gtgccgacgg tgacggcgtt gatgttggac gggagcgcga
     6421 gcggctggcc gccggcggcg aggacggggt agccctcgct ggtgacgagc tggtgctgcg
     6481 catcgagttg gaaatggccg gcgcgcgtgt agcgcgtgcc gagcggcgtc tcgaccgaga
     6541 aataaccggg gccctggagc gcgaagtcga gcggatttcc ggtcttggag atcgggccct
     6601 ggcgcagatc gcgcgccgtg cccgcatcct cgacgaaggc tatcgtgccg gtgccgcgcg
     6661 tatgcgtcag atgctgggtg aacagcatct cctcggcctt gtagccgggc gtcgagagat
     6721 tggccaggtt gttcgccacg acgtccatct gccgctgcaa ggcggtctgg ctggagagag
     6781 agatggaggt tgcgttttgc atcgggtcat ggtgatgcac ggcctgtgcc agggatgctc
     6841 cgacgattgc tgcagcgctg ttaggtgtcc cgcgacacgc tcagcacgag gttttttctt
     6901 tgcggcatcg gcggttgtcg atgccgtcgg cgacctcgtc atgccgagcc tgtcgacgga
     6961 cgcaaggccg gcggtggagc ggggccggca cgctttgccg agcacttcct gcccgccgga
     7021 gcgtgcagtt gtatgtgaaa tcatggcttc aatcagacgt ttcaatcaga tgttaacgct
     7081 gttctaccta ttctcttcag cgtccactgc ctcccgcaca gataaattgc aatcatggcc
     7141 gatcgcgcgc tcgatgatgc tgctgaagtt ccggcgatgc tgcccgaggg acaagcgcca
     7201 gcgccggcgg gcggccgcaa gaagctcatc atcatcgtcc tgggtgccgt gctgctgctg
     7261 gcgggggcgg ggggcggcct ctatttcacc ggcatcgtcg acaaattcct gccgcacaag
     7321 gccgaggtcg tggacaaggc ggcggcgccg gcagcgccgc cggtgttctt cgacatgcag
     7381 gacctgctgg tgaaccttaa caccgtcggg cgcaagcagt ccttcctcaa gttgtcgatc
     7441 agcctggagc tcgagcgcgc cgacgacctg ccgcgcctgc aggcggtgat gccgcgcgtc
     7501 atcgacacgt tccaggtcta tttgcgcgag ctccgcgtcg acgacctccg gggctcggcc
     7561 ggcatctatc ggctgcgcga ggaactcctg acgcgcgtca acgcggcggc ggcgccggtc
     7621 aaggtgaccg acgtgctctt caaggaaatg ctggtgcagt agcgcgatca caatggccga
     7681 gccgaaaccc ccgatcgacg aagagaagct cgcccaggaa tgggcggcga tggcgagcac
     7741 tgagggcgtg cagggcgaca aggccgtggc cgaaggcgag agcgaggccg cggtcaccgc
     7801 gcgcatcctg aaccagaacg agatcgacag ccttctgggc ttcgccgaca gcgacaactc
     7861 ggccaaggaa ggctcgggca tccgcgcgat cgtcgactcg gcgctcgtct cctacgagcg
     7921 gctgccgatg ctcgaggtcg tgttcgaccg gctcgtccgg ttgatgtcga cctcgctgcg
     7981 caacttcacc tcggacaacg tcgaggtctc cgtcgataac gtcacctcga tccgcttcgg
     8041 cgattacctc aattcgatcc cgctgccggc gatgctgagc gtcttcaagg ccgaggagtg
     8101 ggacaactac gcgctcctca ctgtcgacag ctcgctcatc tactcgatcg tcgacgtgct
     8161 gctgggcgga cggcgcggcg ccggctcgct gcgcgtcgag ggccgcccct acacgaccat
     8221 cgagcggaac ctcgtcgaac ggctggtggc ggtcgtgctt tcggacctct cgggcgcgtt
     8281 cgaaccggtg agccccgtga ctttccgctt cgagcggctc gagaccaatc cccgcttcgc
     8341 gaccatcggg cgcccggcga acgccgcggt gctcgcgcgc ctgcgcatcg acatggagga
     8401 tcgcggcggc cgtctcgagg tgctgctgcc ctatgcgacg ctggagccgg tgcgcgagat
     8461 cctgctgcag ggcttcctcg gcgagaaatt cggccgtgac tcgatctggg aggcgcatct
     8521 cgccggcgag ctctggcaca cgagcgtgcc gatcgaggcg gtgctcgata ccgtcgagct
     8581 gccgctgcat gaagtgctcg cgctcaaggt gggctcgcgc atcatcctca acgccaacat
     8641 caattccgat gtcggtctgg tctgcggcga cctgccgatg ttcagcggca agatgggccg
     8701 ccgtggcggc aatatcgcgg tccgcatcac cgagaagacg gagaagaagg acaagggacg
     8761 cacgccatga cgctcgctct cgccggggac ctcatcgtcg ccgttctgct ccttgtcacc
     8821 atcgtctact cgaccgtgct caaccgccgc ctcgcggcac tgcgcggcga caaggacgag
     8881 cttcacgcgc tggtggaagg cctggccaag accacggcga gcgccggcgc cggcatcgcc
     8941 gccctcaaag cggccgccga gacggtcggc cgtgagctcg aggcggggcg cgcgaaggcc
     9001 caagcgctac gcgacgatct tgcctacctg gtcgagcgcg gcaacagtgc cgccgatcgc
     9061 ctcgaaggcg ggatccgcgc ccagcgccag gcggtgcgtg ccgccgagac gccggcagcg
     9121 gcagcgccga cgcccatcgc tggtgcgtcg gcctcgcgcg ccgagcgcga tctcatgcgt
     9181 gccctggcgg ggcagcggca atgacggcgc ctcggcttgc cccccacgct gaccctcccc
     9241 ctcaaagggg aaggggacgc tgtttcgcag agcatcggcc gtagctgcca tggcgtaccg
     9301 cgtccgtgtc atgccgctgg caatcctcgc ctcgatcggc ctgctcggga tcaagctcgg
     9361 cgacctgtcc tcgctcgtgc tcacgacctc gatcgcgcgc gcagagggcg ccactccggc
     9421 ggcgccggca cctgccgccg cgacgcctgc gccggccgcg accgacgccc attcgccccc
     9481 atccgccccg ccggcggcta cggacatcga tccgctgtcg atgagcccgt ccgagatcga
     9541 cctgttgcag aagctggcgg agcggcgcac cgagctcgac aagcgcgccg ccgacctgag
     9601 ccagcgcgag atcctgctgc aggcggggga gaagcgcgtc gacgagaaga tcgttaagct
     9661 ggccgcgctc gagaaggaca tcgccgacat cgtcgagaaa cagaacgacg agaccgagca
     9721 gcgcaccaag agcctggtca agatctacga gaccatgtcg cccaaggatg ccgcgcgcat
     9781 cttcgagcag ctcgacatgg gcgtgttgct ggccgtcgtc gagcatatga aggagcggaa
     9841 cgccgcgccc atcctcgccg ccatggacgc cgccaaggcg cggacggtga cccaggccct
     9901 ggcggagcgc agcgaccggc accccaaggt cgacggttcc acgaagaccg cggccaaacc
     9961 ctgatccggt taacggaatc tcaaccacgg atggggtaaa ggtcttgcaa atgcagcgca
    10021 agccgtcgcc tccaggagct cccgcgatga agtccgccga cgacggcgat ctcgcgcgca
    10081 agctcgaagc cgtccagtcg cggtcgccgt cgacggtcga gacgagcgag gtggcctcga
    10141 tcgtcgaatc cattctcgag agcctctcgg gcgacatgtc ggtcgcggat ctcaagctct
    10201 atcgcgagct cgagcacctc gccggctata tccatcgcgc caagcgcgag atcgccgagc
    10261 ttcatccgca cgagatccgc gagcgccaca tcccggcggc gactgacgaa ctcgacgccg
    10321 tggtcgacgc gaccgccgcc gccacggggc acatcctcga cgaggccgag gcgatcgaga
    10381 agctcaaggc gggactgccg cccgagaccg ccatcctgat cgccgccgcg gtcgcgcgca
    10441 tttacgaggc ctgcagcttc caggacatca ccggacagcg catcaacaag gtcgtgaaga
    10501 cgctccgcta catcgaggac aagatcgacg ctctcctgag cgcgttcgac ggcggcgtcg
    10561 gcggcgagcc cgcggcggcg ccgatggggc cgtcgaaggc gccggacgag gaattcatca
    10621 gcggcccgca gcttcccgac aacgccaaca accaagccga tatcgacgcc atcctggcaa
    10681 gcctcgacaa gcaatgatcc gacgcagcgc gctcgccgtc gcgctgctga tccttgcgct
    10741 gacgggagcc acggccgggg ccaaggacgc gcctccctcg cgcgccgtag cggcttccac
    10801 cgggcatctc catttcagcg agcatcgcga tttccggcgc gtgatcttca actggcacga
    10861 gcccgtcgcc tatgtgctca tcggcgcgga aggcgtggcg cggctgcgct ttgtcggcgc
    10921 ggcgcagctc gatgcggccg agctccaggc ggcgcttccc gatctttcgc cgacgctgcg
    10981 cgcgcagggc aacacgacgg tcgtttcctt ccgcttgccg gcgggcgtca agctccgcca
    11041 tttccgcagc ggcacggcga tcgtcgtaga cattctgcgg ccgggcgccc cgtccggccc
    11101 gcgcaccccg gcgccggcgg ttcaggcgct atgcggtccc agcggctctt gtcccgagct
    11161 gcagacggcg aaagccgagc tcaagacggc gacggccgag ccccggcccc aaccggcacc
    11221 cgcgccggcg gacagcggga aaggggatac ggacaagccc gctgctccag gcaacccgat
    11281 tgatctcgcc cccgcggcac aagcggtggc cgccaccgcc gcggccgacg cgctgctcgt
    11341 tcagggtgca gcgacgccgg aaggtgttcg cttgcgcttc gattggccga agccgaccgc
    11401 ggccgcggtg ttccgacgtg ccggcgcggt gtggatcgtc ttcgcgacgc cggcggcgct
    11461 cgatctcgcc gagccgcgga agctcgttgg cggcgcgttg tcgacgctcg atcagatcgc
    11521 ggacgaacgg gcgacggtgc tccgtctcgt gccgcgcgcg ggccttgctc cgagcgtgcg
    11581 gcgcgacggg aattcctgga tcgtcgagct caaggcgcag gacgcccagc cggacacgcc
    11641 gctcaatgtc gagacgcggc ccgccgccga tacgccgagc gtcctcttcg cgctcacgga
    11701 cgcggctgag ccgctgcggc tgcgcgaccc cgagctcggc gacagcctcg tggtcgtgcc
    11761 gctcaccgcc gccggcaccg gcgcgctcga gcaatccttc gtcgatttcc gcgtcctcgc
    11821 cagcgtccag ggcctggcgc tccgccccaa tgtcgatgac ctgatcgtgg cgagcacgct
    11881 ggacgggctc gcggtgacgc ggccgggcgg cctgctgctg gccggcaaga tcgagcatcg
    11941 gcttgcaaac ctggccgatg gcgggctgcg cctcctcgat ttcgcgagct ggcgcggcga
    12001 gcacgatgcg aatttcctgc ggacgcgcag ccgcctcgag catctcgtga cgacggcatc
    12061 gccgggtgag cgctcgcgcc gccggctcgc gctggcccat ttctacttcg cgaatttctt
    12121 cgccgccgag gcggagggcg tgctcgaggc catccgccgc gacgatcccg atctcgccgc
    12181 cgatcccgcc gtcgcgctga tgatcggcgc ggccgagctc ctgaccggcg accggcgggc
    12241 cgcagcgcag gatctcggcc gccaggttct cgacggccag atcgaggcga cgttgtggcg
    12301 cgcctcgctc gcggccgaga cgggcgactg gccggcggcg gcgcggggct tcatcaccac
    12361 ggtgagcctg ctgccgctct atccgaagcc gctgcgcgac cgttttgcac tggaagcggc
    12421 cgaggcgctg ctcgagacga gccagccgga cgcggtaccg ccgatcctcg atctcgtgct
    12481 cggcggccgg ccgagccggg ccgatgaggc gatggcgctc tatctcgacg gcaggcgcgc
    12541 ggatcagcaa ggcgaccgcg cgcacgcgat cgagcgctgg acggcggtcg ccggcatggg
    12601 cgagccgcgc agccgcgtgc gttcgctcat cgcgcgggcg acggcgcagc tcgagacgaa
    12661 ggagatcagc cgggccgacg cgatcaaggt gctcgacggt ctgcgcttcg actggcgcgg
    12721 cgacttcgtc gagttcgaac tgctgcgccg cctcggcgag ctgcaattgg cggaaggcga
    12781 ccggcgcgac gcgctcgaca cgctgcgcga agccgcggtc aacttcccag actatcccgc
    12841 ggccaaagcg gtgatgacgg agctgagcga tgccgtggcc gagatgttcc tcggcgacgc
    12901 catgaagaac ctgtccccgc tcaaggcact ggcgctctac gacgagttca aggacttcac
    12961 gccggtcggc gagcgcggcg atcggatcct gagcgggctc gtcgaccgcc tcgtggccgt
    13021 cgatctgctc gaccgcgctg ctgcgcttct cagcgagcag atcgagcacc gccttcaggg
    13081 tcacgacaag gcgcgggccg cgacgcagct cgcgctgctg cgccttctcg accacaagcc
    13141 cgaggccgcg ctccaggcgc tcgccatccc gatcgaccgc gatgcgccgg tggagctgct
    13201 gcgccagcgc gtgcagctgc gcgcccgtgc gctcacggat ctccagcgtc ccgacgaggc
    13261 gctcgccgtc atcgccgatg accacagccg cgacgccgat cgcctgcgcg ccgacatctt
    13321 ctggcacggc cagaaatggg gcgaggtggt gacggtgctg tcgcgtcttg cgccgacacc
    13381 gccgagcgac ggcaagctcg acgatgggtc gacccagctc gtgctgaacc ttgccgcggc
    13441 gatgatcctg gccggcgaca aggcgggcct gtcacagctc cgcaccgtct acggcaaggc
    13501 gatgagcggc acccccagcg gcgatgcctt ccatgttctc gccggcgaat cggcgcccgg
    13561 cgcgaaagac gtcgacccgc gggaatttgc cagccacgtc gcgcagctca gcgagctcca
    13621 gggcttcatg acgtcgctca ggggtcctcc ggcaaagaac tgaggcggcg cgaaaagcct
    13681 attgcgccgc gtcgccgccg tagaaggcct gggcgtcgtc gtggaggcgg tggcgcgagc
    13741 cggtgcgcag atacatcatg tggccgccgg gatagagctt gaggtcgatg cgctgcccgg
    13801 tgatcccgcg cggcatgtga tcgacaacat agcggctcat catgtagggc gtcgtcaggt
    13861 cggtcaggcc atgggcgatg agcacgcgga gccgcggatt gagcgcgagc gcggtcctga
    13921 gcgcctcgct ggcgccgagc gtctgccggc cgccgccatg ctcgccgtcg cgccactccc
    13981 agtgatggtt gacctcgtcg ttcaggagct cgtagccgag ctccgtctcg aagccgagct
    14041 cggtcctgac ataagcgacg aaggcctggg tgaagggcgc catgcttccc tcgagcacgg
    14101 gatcgtcctc cgcatcgcgg ctgcgggggt cgggatcggg cgcggcgatg ccgccgtcat
    14161 agcggctcaa cacctgctcg tcgcgatgac gcatctcctt gacatagacg ctgacgggca
    14221 cgcggccctc ccagcgcgcc acggcctcag tgtcgaggcc gatgagcgcg ccgatccgcg
    14281 catagagcgg ttgcgccgcc gcggcgtcgc cggggccggc ggcgagcgcc gtgagatagt
    14341 cgccgagggc gaaacgctcg gcatcgacca gcgcctccgg cgtcaacgcg cccttggcct
    14401 cgagcgccac cgcggcatag gcgggcaggc ggagcaccca gggaatggga tcgaactcgt
    14461 cgccgctcat caagcggaac tcgagcaccg gcgagatcag cagcacgccg gtcgcggcga
    14521 cgccctggcg ctgcccgagt tggtgggcga gccgcgcggc acggaagccg ccatagctct
    14581 cgcccgccag atagaccggc gacgtcaagc gcccgacgcg ggtcaggtgc acgcgaatga
    14641 tttgcgccag cgcgtcgaga tcggcgcgca cgctccacag ctttcgcgcc tcgtcgctgc
    14701 cgccgacggc gcggctgtag cccgtgccga ccggatcgat gaagacgaga tcggtgaagg
    14761 cgagccagct gtcgggattg tcgacgacgt gattgaccgc cggctgcggc ctgccggcgg
    14821 gcccgaaatc gagcgcgcgg ggtccgagcg cgccgatgtc gagataggcg gccgcggcgc
    14881 caggcccgcc gttgaacgcg taagtgatgg gacgcggctg cgcatcggcg acgccctcgc
    14941 gaagaaaggc gacgtagaag atgtcggcct gcttctcgcc tttctcgtcg cgcagcggca
    15001 gcgtgcccgc ggtcgcggtg taggcgatgt cctgtccgcc gagcctgatc ttgtgatgcg
    15061 tcacggcgtc gtgcggcagc gccgaggacg ccgcggccga cggtttcgcc ggtgtatcgg
    15121 aggccggctt gtcctcggcg cgcaacggcg acgcgagcgc caggacgccg ggaaggagag
    15181 cgaggatcga gagcgatcgg cgagtgagac ggcgcatggc gacctcgctg gcggcggcgc
    15241 cgtggagact agagcagatc gggatcgagt ggtatcggca cttccggcgt cgccgttccc
    15301 gcgaacggga cggtgctcta ttcgagcgga atgcgctcta gtcttactgc gtcacaaatg
    15361 gagggtcttg ctgcgagcga ttttccggac gcagcaaggg catctcgaca agcgcctggc
    15421 gagagcgacg atcattcgcc ggcgcagggt tgcgatcgag ttggggatgt ggcg
//
LOCUS       DASYNS010000034         2122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3129816, whole genome shotgun sequence.
ACCESSION   DASYNS010000034 DASYNS010000000
VERSION     DASYNS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3129816"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VGP50_01090"
     CDS             complement(<1..569)
                     /locus_tag="VGP50_01090"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV8025988.1"
                     /translation="MTEGMTQIEHARRLAAVLAMDMVGYSRQMERDEADTLTRFNALR
                     DAVIEPEIGRHRGRLVKYTGDGCMLEFSSAGDAADCALAIQEAVAKRNAGLAEPQQIR
                     FRVGINLGDVIPEQNEIHGDGVNLAVWLESIAPPGGIAISAAVHEQIAGRKLYRFVDL
                     GYRRSQKVERLIRVYRLCLPGEEGSQGWAL"
     gene            complement(763..>2122)
                     /locus_tag="VGP50_01095"
     CDS             complement(763..>2122)
                     /locus_tag="VGP50_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025989.1"
                     /translation="AVHEQIAGRKLYRFVDLGYRRSQKVERLIRVYRLCLPGEEGSQG
                     WALGGVVASVEQPRLSILALPFRTLGGTPEDEYFAEGLTEDVITDLSRIPGSFVIARN
                     TAFAYKDKPIDVLAIGRELNVRYVLEGSVRRASRKVRVNAKLIDAVTEANVWAGRFDS
                     DERDLLDLQDELTGRIAASLDYQLTDAEARRTLPERPDNLNAVDHTLRGWSIVNRPTT
                     RENLVAARRCFEAALNLEPDYAHALVGLAETHVYDVAHGYSAEPRAQLRQAEQVILRA
                     LALDPRHARGYNVRGIQLRFALQFDEALAACERALGLNRNLASAHVQIAWVKQLTDRP
                     QETFGHIEKAMRLSPRDPSLAAWLALLGSAHISLKQDHQAIEVLHRAVNENPLLSSAY
                     QLLAAAYALTGQIDAARAALAEFNRLIPNMTIRRSLEAWSVLTRFHRLTKGLQLAGMP
                     PE"
BASE COUNT          368 a          723 c          692 g          339 t
ORIGIN      
        1 agcgcccagc cctggctccc ctcctcgccg gggaggcaca ggcgatagac gcggatcagc
       61 cgctccacct tctgcgagcg gcgatagccc agatcgacga agcggtagag cttccgtccg
      121 gcgatctgct cgtggaccgc cgccgagatc gcaatcccgc cgggcggcgc gatgctctcg
      181 agccacaccg cgagattgac gccgtcgcca tggatctcgt tctgctccgg gatcacgtcg
      241 ccaagattga tgccgacgcg aaagcggatc tgctgcggct ccgcaagacc ggcattccgc
      301 ttcgccaccg cctcctgaat cgccaaggcg cagtccgcgg catcgccggc gctcgagaat
      361 tcgagcatgc agccatcgcc ggtgtacttg accagacgcc cgcgatgccg gccgatctcg
      421 ggctcgatca ccgcatccct gagcgcattg aagcgggtga gcgtgtccgc ctcgtcccgc
      481 tccatctgcc ggctgtagcc gaccatgtcc atggccagga cagcggcgag tcggcgcgca
      541 tgctcgattt gcgtcatccc ctcagtcacc gcgcaagctc cccacagccg cgccattcag
      601 cgcaattctt aggccagagt ccgatgcctg cccgccaggg cgaaaaacat ctgaccgcgg
      661 agtctgatca aaacagtgta catcgcgcag aggtggaggc aacaccccgt tcaatcccgc
      721 gttaagtgcg cgggaactcc ggccaagcgg cggggaaaat gctcattccg gcggcatgcc
      781 ggcaagctgc aggcccttgg tcaaccggtg gaagcgcgtc aagaccgacc aggcctccag
      841 actccgccgg atggtcatgt tcggaatgag gcgattgaac tccgccagcg cggcgcgcgc
      901 cgcatcgatc tggccggtca aggcataagc cgcggccagc agctgataag cggacgaaag
      961 cagcgggttc tcgttgaccg cccgatgcag cacctcgatg gcctggtgat cctgcttgag
     1021 cgagatatgc gccgagccga ggagagcgag ccacgccgcg aggctggggt cgcgcgggct
     1081 caagcgcatg gctttctcga tatggccgaa ggtttcctgc gggcggtcgg tgagctgctt
     1141 cacccaggcg atctggacat gggcactggc gagattgcgg ttgagcccga gcgcccgctc
     1201 gcaggcagcg agagcctcgt cgaattgcaa ggcgaaacgc agctggatgc cgcgcacatt
     1261 gtagccgcgg gcatggcgcg gatcgagcgc gagcgcgcgc aggatcacct gctccgcctg
     1321 tcgaagctgg gcgcgcggct cggcgctgta gccgtgagcg acgtcgtaga catgcgtctc
     1381 ggccaggcct accagcgcat gggcatagtc gggctcgaga ttgagcgccg cctcgaagca
     1441 gcgccgcgcc gccacgaggt tttctcgcgt cgtcggcctg ttcacgatcg accagccgcg
     1501 gagcgtgtgg tcgacggcgt tcagattgtc cgggcgctcg ggaagagtgc gccgcgcctc
     1561 cgcatcggtg agctgatagt cgagcgaggc ggcgatccgg cccgtcagct cgtcctgaag
     1621 atcgaggaga tcgcgctcgt cgctgtcgaa gcgcccggcc cagacattgg cctcggtcac
     1681 cgcgtcgatc agcttggcat tgacgcgcac cttccggctc gcccgccgaa cgctgccttc
     1741 gagcacatag cggacgttga gctcgcggcc gattgccagc acatcgatcg gcttgtcctt
     1801 gtaggcaaag gccgtgttcc gcgcgatcac gaaactgccg ggaatgcgcg acaggtcggt
     1861 gatcacatcc tcggtcaagc cttcggcgaa atactcgtcc tccggcgtgc cgcccagcgt
     1921 gcggaacggc agcgccagga tcgacagccg cggctgctct accgacgcca cgacgccgcc
     1981 cagcgcccag ccctggctcc cctcctcgcc ggggaggcac aggcgataga cgcggatcag
     2041 ccgctccacc ttctgcgagc ggcgatagcc cagatcgacg aagcggtaga gcttccgtcc
     2101 ggcgatctgc tcgtggaccg cc
//
LOCUS       DASYNS010000035         6580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2818243, whole genome shotgun sequence.
ACCESSION   DASYNS010000035 DASYNS010000000
VERSION     DASYNS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2818243"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VGP50_01100"
     CDS             complement(<1..194)
                     /locus_tag="VGP50_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8025990.1"
                     /translation="MNIKTLAAGAAALLAIAAAAIYGVDAYAQREASNQVEASFAHLP
                     RSMQGRHGAVSYSVLGDRLVL"
     gene            complement(323..1081)
                     /gene="moeB"
                     /locus_tag="VGP50_01105"
     CDS             complement(323..1081)
                     /gene="moeB"
                     /locus_tag="VGP50_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEV8025991.1"
                     /translation="MLTDAQFERYARHLILEEVGEEGQARLMASRVLVVGAGGLGSPL
                     LLYLAAAGVGTLGVIDDDTIDLSNLQRQIIHPTERIGMRKVDSARQTLAAVNPEVVVE
                     THALRLDASNAAALIARYDLVADGSDNFATRYLLNDACFAERRTLVGAALSPFDGQLS
                     TFKAYLGPPHPCYRCLFREPPPADLVPRCETAGILGAVAGVVGTLQATEVLKELLGLG
                     DSLSGTLLLYDALRAGFHKIALPRDPDCPTCGRR"
     gene            complement(1163..2155)
                     /gene="cysK"
                     /locus_tag="VGP50_01110"
     CDS             complement(1163..2155)
                     /gene="cysK"
                     /locus_tag="VGP50_01110"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV8025992.1"
                     /translation="MAQPQAQDKSKVPVTGEFRGRVYDSILETVGATPLVRLSRLAKA
                     HDVRADILGKCEFFNPLASVKDRIGLAMVEAAERTGKIRPGKTVLVEPTSGNTGIALA
                     FVCAAKGYRLILTMPDSMSVERRKMLKLLGAELELTPAAQGMRGAIARAEELMRSFPD
                     SFMPQQFDNPANPEIHRRTTAEEIWRDTDGRVDAVVSGVGTGGTLTGVGDVLKSRKPD
                     VRMIAVEPEDSAVLSGGMPGPHPIQGIGAGFVPSTLNTKLIDEVVRIANETAFRMARE
                     VARVEGLPVGISSGAAIAAALEVGARRDMAGKMIVVILPSFAERYLSTALFVGL"
     gene            complement(2249..2704)
                     /locus_tag="VGP50_01115"
     CDS             complement(2249..2704)
                     /locus_tag="VGP50_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV8025993.1"
                     /translation="MLRLTKKLLFAIEAVLDIAYNAGPLPVQSAEITRRQGIPRRYLE
                     QVLQQLVRAGVLTGVRGPRGGYRLARERRRITVGEIARVVRSLESGEDPIAGEGGAEL
                     GHKVVRPLWLELQEELMQRLDGTTIEDLCIRAHRAGVAGEYAARFDFTI"
     gene            complement(2723..3982)
                     /gene="coaBC"
                     /locus_tag="VGP50_01120"
     CDS             complement(2723..3982)
                     /gene="coaBC"
                     /locus_tag="VGP50_01120"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEV8025994.1"
                     /translation="MAPILEVRASADPEVILSLQGRRILLIIAGGIAAYKSLDLIRRL
                     RERGAAVRVIMTKAAQQFVTPLAAAALSAERVYSDLFSLTEESDMGHLRLAGEADLVV
                     VAPATADLLAKMAAGLADDLAAAALLATDKPILIAPAMNFRMWEHPATRTNVALLGAR
                     GVHRVGPFAGALAEGESGMGRMAEPLEIAATVEQILGTGALLRGRRAIVTSGPTHEPI
                     DPVRYIANRSSGKQGHAIAAALAGLGADTVLVSGPTVEPDPRGVTVVHVETARDMLAA
                     CEKALPADIAVCAAAVADWRAEAAPQKIKKKNGPPPALKLVENPDILATLSKPGNRRP
                     RLVVGFAAETEQVIEHAKTKRLKKGCDWIVANDVSPATGTFGGDSNTVHLVDEAGVED
                     WPPLSKREVAQRLALRIANFLERKAAE"
     gene            complement(4075..5631)
                     /gene="ubiB"
                     /locus_tag="VGP50_01125"
     CDS             complement(4075..5631)
                     /gene="ubiB"
                     /locus_tag="VGP50_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HEV8025995.1"
                     /translation="MVAVLRAGRNLVRLVEIAIILARYDALLALESVIGPRLALRPLR
                     FLRRRDAALARLRPGQRLAAALEAMGPSFIKLGQALSTRADLVGEAVATDLSSLQDRL
                     PPFPGSAARQMIEAELGQPVSVLFRQFDDTPVAAASIAQVHLALTFEGEEVAVKVLRP
                     DIAAAFARDLDLFFWLAQLIERTQPALRRLKPVAVVQTLADTVRQEMDLRLEAAGASE
                     LRENFAGDATFRVPRVDWARTGRGVLTLERVGGIRVDDRPALIAAGRDVHAVMANAAG
                     AFFNQVFRDGFFHADLHPGNLFIDATGAIVAVDFGIMGRLDRKTRYYLADMLLAFLQG
                     DYRRVAEVHFEAGYVPARQSLEAFAQACRSIGEPILGRPLHEISLGRLLAQLLQVTKQ
                     FQMETQPQLLLLQKTMVLAEGVGRLLDPSVNMWTLARPLIEGWMRENRGPEARLRDAA
                     ADLSGALSRLPALLGGLERLGAAIVEGGGLPLHPETVAAIARRRAVELLGLPLWIMAL
                     ALAAIAVALW"
     gene            complement(5661..6443)
                     /locus_tag="VGP50_01130"
     CDS             complement(5661..6443)
                     /locus_tag="VGP50_01130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8025996.1"
                     /translation="MLARYGGAMAGSETGGEADFGYRRVKAAEKPFLVRQLFDGVASR
                     YDLMNDLMSGGVHRLWKAELIQRLNPRPGQVLIDVAGGTGDIAGRFLDSAGASARAIV
                     CDLTPGMLQQGRARAVDRGLLAGIDWVCGDAEQLPIAAGAVDAYTIAFGLRNVTHVAA
                     ALSEARRVLKPGGRFLCLEFGRVEVPVLRQIYELYSFTVLPRVGAVVAGDRDAYQYLV
                     ESIRRFPPQPELARMIGDAGLEQVSVRNLSGGIAALYSAWRL"
     gene            6465..>6580
                     /locus_tag="VGP50_01135"
     CDS             6465..>6580
                     /locus_tag="VGP50_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEV8025997.1"
                     /translation="MPELPEVETIRRGLALRMEGRRVDRLTLNRADLRAKLPA"
BASE COUNT         1117 a         2199 c         2264 g         1000 t
ORIGIN      
        1 agcaccaggc gatcaccgag gacggaatag gaaacggcgc cgtggcggcc ctgcatgctg
       61 cgcggcaggt gggcgaaact cgcctcgacc tggttcgacg cctcgcgctg cgcgtaagcg
      121 tcgacgccat agatcgccgc cgcggcaatg gccagcaggg cggcagcgcc cgcggccagc
      181 gttttgatgt tcatcttgtc cctcggatgg cctttcgccc gagcccggtg ctcgccgccc
      241 cagtgtcgtt catcgccgat gaacagttgg taaaaacggt gcggggtgcg agctcagccg
      301 gtgattgcgg gaaatcgaga tctcagcggc ggccgcaggt cgggcagtct gggtcgcgcg
      361 gcagcgcgat cttgtggaag ccggcgcgga gcgcgtcata gaggaggagc gtgccgctca
      421 ggctgtcacc caggccgagc agctccttca gcacctccgt cgcctgcagc gtaccgacga
      481 caccggcgac ggcgccgaga atgccggccg tctcgcagcg cggcaccagg tcggccggcg
      541 gcggctcgcg aaagaggcag cgatagcagg gatggggcgg gccgagatag gccttgaagg
      601 tggaaagctg gccgtcgaaa ggcgagaggg ctgcgccgac gagcgtccgt cgctcggcaa
      661 aacaggcgtc gttgagaaga tagcgtgtcg cgaagttgtc gctcccgtcc gcgaccagat
      721 catagcgagc gatcagcgcc gctgcgtttg acgcatcgag gcgcagcgcg tgggtctcga
      781 cgacgacctc gggattcacg gccgccagcg tctgtcgcgc gctgtcgacc ttgcgcatgc
      841 cgatgcgctc cgtggggtga atgatctggc gctgcaggtt cgagagatcg atcgtgtcgt
      901 catcgatgac gccgagcgtg ccgactccag ccgcggcgag atagagcaga agcggcgatc
      961 ccagtccacc ggcgccgacg acgaggacgc gcgaggccat gaggcgggcc tggccctcct
     1021 cgcccacctc ctcgagaatg agatgacggg cgtagcgctc gaactgtgcg tccgtcagca
     1081 tgttccgaag cttggaaaac caggagccag gacgatcgat agaaatcgat gcattacccg
     1141 actcccggct gccggctcct gcctatagtc ccacgaagag ggcggtcgag agatagcgct
     1201 cggcgaagct gggcaggatc acaacgatca tcttgccagc catgtcgcgg cgcgccccga
     1261 cctcgagcgc cgcggcgatt gccgcgcccg acgagattcc gaccggcagg ccctcgactc
     1321 gcgccacctc ccgcgccatg cgaaaagccg tctcattggc gatgcgcacc acctcgtcga
     1381 tgagcttcgt gttgagagtc gaggggacga aaccggcgcc gatgccctgg attgggtgcg
     1441 gaccaggcat cccgcccgac agcaccgcgc tgtcctccgg ctcgaccgcg atcatccgga
     1501 cgtcgggctt gcgcgatttc agcacgtcgc cgacacccgt cagcgtgccg ccggtgccga
     1561 cgcccgagac caccgcgtcc accctgccgt cggtgtccct ccatatctct tccgcggtgg
     1621 tgcggcggtg aatctcggga ttggcgggat tgtcgaattg ctgcggcatg aaggaatcgg
     1681 ggaagctgcg catcagctcc tcggcacgcg caatcgcgcc gcgcatgcct tgggcggcgg
     1741 gcgtcagctc gagctcggcg cccagcaact tcagcatctt gcgccgctcg accgacatgc
     1801 tgtcgggcat ggtgaggatg agccgatagc ccttcgcggc gcagacgaag gcgagcgcga
     1861 tgccggtatt gcccgatgtc ggctcgacca gcaccgtctt gcccgggcgt atctttcccg
     1921 tgcgttccgc cgcctcgacc atggcgagac cgatgcggtc cttgaccgag gcgagcgggt
     1981 tgaagaactc gcatttgccg agaatgtcag ccctcacgtc gtgcgctttc gcgagtcgcg
     2041 acagccgcac caagggcgtg gcgcccactg tctcgagaat gctgtcatag acgcgcccgc
     2101 gaaactcgcc ggtcacgggc actttggact tgtcttgcgc ttgtggctgc gccatcccgg
     2161 gcctccgcac gttgcattcg gccaatcagg ccttgtagac cctttcgacc gagcccgcaa
     2221 cgcaagccgg atggcgtaat tcggacggtc agatcgtgaa atcgaagcgc gcggcgtatt
     2281 cgccagcgac gcccgcgcga tgggcgcgaa tgcagagatc ctcgatggtc gtcccgtcga
     2341 ggcgctgcat cagttcctct tgcagctcga gccagagagg acgcacgacc ttgtgcccga
     2401 gctcggcacc gccttcgccg gcgatcggat cctcgccgct ctcgagcgaa cggacgacac
     2461 gggcgatctc acccacggtg atgcggcggc gctcacgtgc cagtcgatag ccgccacgcg
     2521 gcccccgcac gccggtgaga acgccggcgc gcaccagctg ctgcagcacc tgctcgagat
     2581 agcgccgcgg gatgccctgg cggcgggtga tctcggcgga ttgcacgggc aagggcccgg
     2641 cgttgtaggc gatgtcgagc accgcctcga tcgcgaaaag cagcttcttg gtgagccgca
     2701 gcatggcgtc gctaggctgt gctcattcgg cggctttgcg ctcgagaaaa ttggcgatgc
     2761 gcaaagcgag ccgctgcgcc acctcgcgct tcgacaaggg cggccagtcc tcgacgcccg
     2821 cctcgtcgac gagatggacg gtgttgctgt cgccgccgaa ggtgcccgtc gccggcgaca
     2881 cgtcgttggc gacaatccag tcgcagccct tcttcagccg cttcgtcttg gcgtgctcga
     2941 tcacctgctc ggtctcggcg gcaaagccca cgaccaggcg cggccggcgg ttgccgggtt
     3001 tgctgagcgt cgcgagaatg tccggatttt cgaccagctt cagcgccggc ggcgggccgt
     3061 tcttcttctt gatcttctgt ggcgccgcct cggcgcgcca atcggcgacg gccgcagcgc
     3121 agaccgcgat gtcggcgggg agcgccttct cgcaagcggc gagcatgtcg cgggctgtct
     3181 ccacatgcac gacggtgacg ccgcgggggt cgggctccac cgtcggtccc gagaccagga
     3241 cagtatcggc cccgagaccc gccagtgccg cggcgatggc atggccctgc ttgccggagg
     3301 aacgattggc gatatagcgc acgggatcga tcggctcatg ggtcgggccg ctcgtcacga
     3361 tggcacggcg ccccctgagc aacgcgccag tgccaaggat ctgttcgacc gtagcggcga
     3421 tctcgagggg ctcggccatc cggcccattc cactctcgcc ttcggccagc gcgcccgcaa
     3481 acgggccgac gcggtggacg ccgcgtgctc cgagaagcgc gacattggtg cgggtagccg
     3541 ggtgctccca catgcgaaaa ttcatggcgg gcgcgatcag aatcggcttg tcggtggcca
     3601 ggagcgcagc ggcggcgaga tcgtcggcga gaccggccgc catcttggcg aggaggtcgg
     3661 cggtcgccgg tgcaacgacc acgaggtcgg cctcgccggc gagccgcaaa tggcccatgt
     3721 cgctctcctc ggtcagcgag aagaggtcgc tgtagacgcg ttcggcgctg agcgccgcgg
     3781 cggccagggg cgtgacgaac tgctgcgcgg ccttggtcat tatgacacgc accgcggcgc
     3841 ccctctcgcg caggcggcgg atgagatcga ggctcttata ggcggcgatg ccgcccgcga
     3901 tgatcaacag gatgcgccgg ccctgcagcg acaatatgac ctccggatcg gccgaagccc
     3961 tgacctccag tataggcgcc atggaaagcg agcgccatcc cggcctgggt ttctcatccc
     4021 gcgccggcgg accagagcgt cacagatcct tcgccctcgt cgccgagttt ctcgctacca
     4081 caacgccacc gcgatcgcgg ccagcgccag cgccatgatc caaagcggca ggcccagcag
     4141 ctcgacggcg cggcgtcggg cgatcgccgc caccgtctcg ggatgaagcg gcaggccgcc
     4201 gccttcgacg atggctgcac cgagccgctc gagaccgccg agcagcgccg gcaatcgcga
     4261 gagggcgccg ctcagatcgg cggcggcatc gcgcagtcgc gcctcgggac cgcgattctc
     4321 gcgcatccag ccctcgatga gcggccgtgc caaggtccac atgttgacgc tcggatcgag
     4381 gagccgcccg acaccttcgg ccagcaccat ggtcttctgt agcagaagaa gctgcggttg
     4441 tgtctccatc tggaactgtt tggtgacctg aaggagctgc gcgagaagcc gccccagcga
     4501 gatctcatgg agcggccgcc ccaggatggg ttcgccgatc gagcggcacg cttgggcaaa
     4561 ggcctcgagc gattggcgcg ccggtacata gccggcctcg aaatgcacct cggcgacacg
     4621 gcggtagtcg ccttgcagga aggccagcag catgtcggca agatagtacc gcgtcttgcg
     4681 gtcgagccgc cccatgatgc cgaaatcgac ggccacgata gcaccggttg catcgatgaa
     4741 gaggttgccg ggatggaggt cggcgtggaa gaagccgtcg cggaacacct gattgaagaa
     4801 ggcgcccgct gcgttggcca tgacggcatg gacgtcgcgg ccggcggcga tcagcgccgg
     4861 gcggtcgtcg acgcgaattc cccccacccg ctcgagtgtc agcacaccgc gccccgtgcg
     4921 cgcccaatcg acgcgcggca cgcggaacgt cgcgtcgccg gcgaaattct cgcgcagctc
     4981 cgacgccccg gccgcctcca agcgcagatc catctcctgc cgcaccgtgt ctgcaagggt
     5041 ctgcaccacg gcaacgggct tcagacgccg cagcgccggc tgcgtgcgct cgatcagctg
     5101 ggcgagccaa aaaaacaggt cgaggtcgcg ggcgaacgcc gcggcaatgt cggggcgcaa
     5161 caccttgacc gccacttcct cgccttcgaa ggtcagggcg agatgcacct gcgcaatcga
     5221 agcggcagcg actggcgtgt cgtcgaactg acggaagagg acgcttacgg gctgcccgag
     5281 ctccgcctcg atcatctgcc gagcagcact tcctgggaaa ggcggcaggc gatcctggag
     5341 gctcgagaga tccgttgcca ccgcttcgcc gacgagatcg gcacgggtcg agagcgcttg
     5401 gccgagcttg atgaagctcg gccccatcgc ctccagggcg gcggcgaggc gctgccccgg
     5461 ccgtagtcga gcgagcgcgg cgtcgcgacg ccgcagaaag cgcaaaggcc tgagcgcgag
     5521 gcgcggcccg atgacgctct cgagcgccaa gagcgcatcg tagcgtgcca gaatgatggc
     5581 gatctctacg agccggacga gattgcggcc ggcgcgcagc acggcgacca tcggctatcg
     5641 ctccgcgggg ccccggccgg tcaaaggcgc caggcggaat agagcgcggc gatgccgccg
     5701 ctgagattgc gaacgctcac ctgctccagg ccggcatcgc cgatcatgcg ggcgagttca
     5761 ggctgcggcg gaaaccgacg gatgctttcc accaggtact gataggcgtc gcggtcgccg
     5821 gccaccaccg caccaacgcg aggcagcacg gtgaaggagt agagctcgta gatttggcgg
     5881 agcaccggca cctcgacccg accgaattcg agacagagaa agcggccgcc gggcttcaac
     5941 acgcggcgcg cttcgctgag tgcggcagcg acatgggtga cgttgcgcaa gccgaaggcg
     6001 atggtgtagg cgtcgacggc gcccgcggcg atgggcaact gctcggcatc gccgcacacc
     6061 cagtcgatgc cggcgaggag gccgcggtcg acggcgcgag cgcgcccttg ctgcagcatg
     6121 cccggcgtga gatcgcagac gatggcgcgg gcggacgcgc cggcgctatc gaggaagcgc
     6181 ccggcgatgt cgccggtgcc gccggcgacg tcgataagga cctgtcctgg ccgcgggttg
     6241 aggcgctgga tgagttcggc cttccatagg cgatggacgc cgccgctcat caggtcgttc
     6301 atgagatcgt accggctggc gacgccgtcg aagagctgac gcaccagaaa cggcttctcc
     6361 gcggctttca cgcggcgata gccgaaatcc gcttcgccgc ctgtctcact gcccgccatg
     6421 gccccaccat agcgcgcaag catggcgagc gctatgtaga ggccatgccg gaattgccag
     6481 aagtcgagac gatcaggcgc ggcctggcgc tccgcatgga ggggcggcgg gtcgaccggc
     6541 ttacgctcaa ccgcgcggat ctgcgtgcga agctgccggc
//
LOCUS       DASYNS010000036         4838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3130557, whole genome shotgun sequence.
ACCESSION   DASYNS010000036 DASYNS010000000
VERSION     DASYNS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3130557"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VGP50_01140"
     CDS             complement(<1..900)
                     /locus_tag="VGP50_01140"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEV8025998.1"
                     /translation="MRQGDHDSSDAALPVASFADPVRLAWAGLISMAAAMGVGRFVYT
                     PILPPMAEALHLSPAQAGWIASANFAGYLAGAMLAAGALPGSARAWLMTGLVASAVST
                     AAMGLTSALTMFALIRFAGGIASALVLVYGSTVVLERLGAAGRSDLAARHFAGVGVGI
                     AASAILVSALEAAGADWRDLWLASGAVSLLAILAVAMLLPAAPRQAAAAPAAIGRATP
                     RARRLSLLFWAYGLVGLGYVITATFLVTIVRGSAAARTVEPVVWVVVGLAAVPSVAAW
                     MRIGRKIGLAEAFALASAIEALGV"
     gene            complement(1045..1296)
                     /locus_tag="VGP50_01145"
     CDS             complement(1045..1296)
                     /locus_tag="VGP50_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEV8025999.1"
                     /translation="MKGDPRKRSVVIAGHATSLTLEEAFWQDLREVARRRGLSVTALV
                     RAVDAGRSGNLSSALRLFVLDCYRRGEIGPAAGERTPAR"
     gene            complement(1317..1784)
                     /locus_tag="VGP50_01150"
     CDS             complement(1317..1784)
                     /locus_tag="VGP50_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV8026000.1"
                     /translation="MSVSLEQTRDLMRRAHAGQVDKAGQPYHTHPERVLERLLRRFPE
                     ASESEQHAALLHDVLEDTDITAEDLAAAGYARDVIEIVSWLTRPPDITYLQWMAQLGE
                     EAPLGALRVKLADNADNSDPVRLAAIPEHERGLSQRYAQARAVLEAALSRRAG"
     gene            complement(1927..3330)
                     /gene="fumC"
                     /locus_tag="VGP50_01155"
     CDS             complement(1927..3330)
                     /gene="fumC"
                     /locus_tag="VGP50_01155"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEV8026001.1"
                     /translation="MADAKATRIERDSMGEIAVPAEHYWGAQTQRSLENFRIGGERMP
                     APLIRALALEKKAAALANMALGELDPKLGRAVIAAADEVIAGKHDGEFPLVVWQTGSG
                     TQTNMNMNEVLANRANELLGGARGAKQPVHPNDHVNRGQSSNDSFPTAMHIAAVEEIQ
                     HRLLPALRHLHAALAAKAQEFADIIKVGRTHLQDATPLTLGQEFSAYARQVEFGIARV
                     EAALPRLHPLAQGGTAVGTGLNARKGFAEAFAAAAAEITGLPFTSAVNKFEALASHDA
                     LLEASGALNVLAASLMKIGNDIRLMGSGPRSGLGELHLPENEPGSSIMPGKVNPTQVE
                     ALTMVAAQVMGNHVTITVAASQGQFELNVMKPVIIYDLLQSIALLADAAQSFADHCVA
                     GIAANRPRIAELLERSLMLVTALNPHIGYDKAAEVAKKALADGLTLKEAAAALGYVTP
                     EDFDRWVRPEAMLGPTQ"
     gene            complement(3425..3748)
                     /locus_tag="VGP50_01160"
     CDS             complement(3425..3748)
                     /locus_tag="VGP50_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026002.1"
                     /translation="MAESAALLLEQARAKRELAAHARQLATAGIPANISADFRQFAAE
                     LDAIAADFEQQARNAAEVAAKSAELAGRAAKLQRALGHAIAEAMKLGGRLRATKPDGN
                     GRSLE"
     gene            complement(3867..4622)
                     /locus_tag="VGP50_01165"
     CDS             complement(3867..4622)
                     /locus_tag="VGP50_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEV8026003.1"
                     /translation="MATILMPLPSKDFDPTESGVPWRVLSDRGHRVVFATPDGRLGDA
                     DPRMVTGEGLGVLARVLMADQNGRSAYAGMAQSAEFHNPIAYDAIAETALDALLLPGG
                     HAQGMRPYLESSRLQSTVTAFFARRKPVGAICHGVLLAARSLVADASVLRGRKTTALT
                     KLMELGAWALTCSYLGDYYRTYRTTVEDEVRTALAQGDDFLRGPVALTRDSPGNLAAG
                     FTVRDGNYLSARWPGDAHRFAHEFAGMVERPDG"
BASE COUNT          756 a         1629 c         1699 g          754 t
ORIGIN      
        1 cacgccgagc gcttcgatcg ccgaggcgag cgcgaaggcc tccgccaggc cgatcttgcg
       61 gccgatgcgc atccaggcgg cgaccgatgg cacggccgcg aggccgacca cgacccagac
      121 gacgggctcg acggtgcgcg ccgccgccga gccgcgcacg atggtgacca ggaaggtcgc
      181 ggtgatcaca tagccgaggc cgacgagccc ataggcccag aacaacagcg agagccgccg
      241 cgcccgcggc gtcgcgcggc cgattgctgc cggcgctgcc gcagcttgtc gcggggccgc
      301 gggcagaagc atcgcgacgg cgagaatggc gagcagggag acggcgccac tggcgagcca
      361 gagatcgcgc cagtccgcgc ccgcggcttc gagcgccgac accaagatcg ccgaggcggc
      421 aatgccgacg ccgacgccgg caaaatgccg ggccgcgaga tccgaacggc cggccgcacc
      481 gagccgctcc agcaccaccg tcgagccgta gaccagcacg agggcgctgg cgatgccgcc
      541 ggcgaatcgg atcagcgcga acatcgtgag cgcgctcgtc aggcccatgg ccgccgtgct
      601 gactgcgctc gcgacgagcc ccgtcatcag ccacgcccgc gccgagccgg gcagcgcgcc
      661 agccgccagc atcgcgccgg cgagatatcc cgcaaagttc gccgaagcga tccagccggc
      721 ctgggccggg ctcagatgca aggcttccgc catcggcggc aggattggcg tatagacgaa
      781 gcggccgaca cccatcgccg cggccatgct gatcaagccc gcccaggcga gcctgaccgg
      841 atcggcgaag gaggcgacgg gcaaggccgc gtcgctcgag tcgtggtcgc cttggcgcat
      901 gccatccgaa cgcgatgtcg tcgcgcgcga ctctatcctg cgccgccgcc aaaggaacgc
      961 cgctgctttg catggcctct cggcggggag gtcatggcgc aaaccgggtc gcgccgccgg
     1021 caaaggcgca ccgtttgcgg cgggtcaacg cgccggagtc cgctcgccgg cagcgggacc
     1081 gatctcgccg cgacggtagc agtcgaggac gaagagacgc agcgcgctcg agagattgcc
     1141 gctccgcccg gcatcgacgg cgcggacgag cgccgtcacc gagaggccgc gccgccgcgc
     1201 gacctcgcgc agatcctgcc agaacgcctc ttcgagcgtg aggctcgtgg cgtggccggc
     1261 gatcactacc gaacgcttgc gcggatcgcc cttcatcgcg gttcgagaaa tctcagttaa
     1321 cccgcccggc ggctcagcgc tgcctcgagc acggcgcgtg cctgcgcgta gcgctgcgac
     1381 aggccgcgct catgctcggg aatcgcggcg agccggacgg ggtcggaatt gtcggcattg
     1441 tccgcgagct tcacgcgcag cgcgcccagc ggcgcctcct cgccgagctg cgccatccat
     1501 tgcaaatagg tgatgtcggg cggacgcgtg agccaggaca cgatctcgat cacgtcgcgc
     1561 gcatagccgg cggcagccag atcctcggcg gtgatgtccg tgtcctcgag cacgtcgtgg
     1621 agaagcgccg catgctgctc cgactcgctc gcctcgggaa agcgccgcag cagccgctcg
     1681 agcacgcgct cgggatgggt gtgataaggc tgtcctgctt tgtcgacctg gcccgcatgg
     1741 gcgcggcgca tgagatcgcg ggtctgctcg agagagacgc tcatccttcg atcggcttgt
     1801 cgttgcgttt cgttctgggg ccaactcccc gctcccggag agtaccctaa gctcgtggcc
     1861 gcggcataac aagacggtgc gcagcccggt tccgccagcg ccgtcgccga tggcgccgac
     1921 cggcggtcat tgcgttggac cgagcatggc ttcggggcgg acccagcggt cgaaatcctc
     1981 gggcgtcaca tagcccagcg cggccgcggc ttctttgagg gtcaggccgt cggcaagcgc
     2041 cttcttcgcc acttcggccg ccttgtcgta gccgatatgc ggattgagcg cggtgacgag
     2101 catgagcgat cgctccagca gctcggcgat gcgcgggcgg ttggccgcga tgccggcaac
     2161 gcagtgatcg gcgaagctct gcgccgcgtc ggcgagcagc gcgatcgact gcagcaggtc
     2221 atagatgatc accggcttca tgacgttgag ctcgaattgt ccctggctcg cggcgacggt
     2281 gatggtgacg tgattgccca tcacttgcgc cgccaccatc gtcagcgcct cgacctgagt
     2341 cggattgacc ttgccgggca tgatcgagga gccgggctca ttctcgggca gatgcagctc
     2401 gcccagaccc gagcgcgggc ccgatcccat caggcggatg tcgttgccga tcttcataag
     2461 cgaggcggcg agcacattga gcgcgcccga ggcttcgagg agcgcgtcat ggctggcgag
     2521 cgcctcgaac ttgttgacgg cggaggtgaa ggggaggccg gtgatctcgg cggcggccgc
     2581 agcaaaggcc tccgcgaagc ccttccgcgc attgagcccg gtgccgacgg cggtgccacc
     2641 ttgcgccagc gggtggagtc gcggcagcgc cgcctcgacg cgcgcgatgc cgaactcgac
     2701 ctggcgcgcg taagccgaga attcctggcc cagcgtcagc ggcgtcgcgt cctggagatg
     2761 cgtgcggccg accttgatga tgtcggcaaa ttcctgcgcc ttcgccgcga gcgcggcgtg
     2821 gagatggcgc agcgccggca gcaggcgatg ctggatctcc tcgaccgccg cgatgtgcat
     2881 ggccgtcggg aagctgtcgt tcgaggattg gccgcgattg acgtggtcgt tgggatgaac
     2941 cggctgcttc gcgccgcgtg cgccgcccag gagctcgttc gcccggttgg ccagcacctc
     3001 gttcatgttc atgttggtct gtgtgcccga tcccgtctgc cacaccacca gtggaaactc
     3061 gccgtcatgc ttgccggcga tcacttcatc ggcggcggcg atgacggcgc ggccgagctt
     3121 cgggtcgagc tcgcccagcg ccatgttggc gagcgccgcc gccttcttct cgagggcgag
     3181 cgcgcggatc agcggcgccg gcatgcgctc gccgccgatg cggaaattct cgagcgagcg
     3241 ctgggtctgg gcgccccagt aatgctcggc gggcacggcg atctcgccca tgctgtcgcg
     3301 ttcgatgcgg gtcgctttgg cgtcggccat gggcacggct cctcgggcct tggggcacga
     3361 ggatcatatg ggatgcggcg gcacgccgtc agaggcggga tggccgcgcg cgacccgagc
     3421 ggctctattc caggctgcgc ccgtttccgt cgggcttcgt tgcccgcaag cggccgccga
     3481 gcttcatggc ttcggcgatc gcatggccga gcgcgcgctg caatttcgcg gcgcgcccgg
     3541 cgagctcggc cgactttgca gcaacctcgg cggcattcct ggcctgctgc tcgaaatccg
     3601 cggcgatggc gtcgagctcg gcggcgaatt gccggaaatc ggcggaaata ttcgccggga
     3661 tgccggccgt cgcgagctgc cgcgcatgcg ctgccagctc gcgcttggcg cgtgcctgtt
     3721 ccaggaggag agcggccgac tctgccacga cgatctcctg cagtggaaca caggggcgct
     3781 gaaagaagag gcagtgtgac ataggagtgc gcaagcaagc gactcgccct ttcgagtggg
     3841 cgggttgctc gcgtatgtcg ctttcctcag ccgtccggcc gctcgaccat cccggcaaat
     3901 tcgtgggcga agcgatgcgc atcgccgggc cagcgcgccg acagataatt tccgtcgcgg
     3961 acggtgaacc cggccgcgag attgccgggc gagtcgcgcg tgagcgccac cggcccgcgg
     4021 agaaagtcgt cgccctgcgc gagcgcggtg cgcacctcat cctccaccgt cgttcgatag
     4081 gtgcgatagt agtcgccgag ataggagcaa gtgagcgccc aggcgccaag ctccatgagc
     4141 ttcgtgagcg cggtcgtctt gcgtccccgg agaaccgagg cgtccgcgac aagcgagcgt
     4201 gcggccagaa gcacgccatg gcagatggcg ccgaccggtt tgcgcctggc gaagaacgcg
     4261 gtgaccgtgg attgcagccg gctcgattcg agataggggc gcatgccctg ggcgtggccg
     4321 cccggcagta gcagggcgtc gagcgcggtt tcggcgatgg catcgtaggc gatgggatta
     4381 tggaattctg cggattgcgc catccctgca taggcgctcc gcccattctg atcggccatc
     4441 agcacgcgcg cgaggacgcc gagcccttcg cctgtcacca tacgggggtc tgcgtctccc
     4501 aaccgtccat ccggtgtcgc gaatacgaca cgatgtccgc gatcgctcaa gacgcgccag
     4561 ggaacaccgc tttccgtcgg atcgaagtcc ttgctcggca gcggcatcag gatcgtcgcc
     4621 atcgccgccc tctcctgcgg gttgactgcc gtccgtaatc aaggtcgggg aggacgtcaa
     4681 gcgcgtgtcg aggtcaccgc aagccgttct cggcccagct tcgtccggta tcgatccaga
     4741 gctcgtcgac ctggccgttg acgtcgaaga agttgacgtg ctgctgatca ttgtcgttgc
     4801 cgcgatcgcc ggcgagtggc gaggacggcg ctgcgttc
//
LOCUS       DASYNS010000037         3641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2194101, whole genome shotgun sequence.
ACCESSION   DASYNS010000037 DASYNS010000000
VERSION     DASYNS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2194101"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VGP50_01170"
     CDS             <1..828
                     /locus_tag="VGP50_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8026004.1"
                     /translation="GGFYAAMTVGVSISFGMLDIVNIAHPAFIILGSYIAYIVNARLG
                     IDPVVASILAAPLFYALGALIYQIYHVAFERRGDEALRGLAFFFGLLFITEVALILVF
                     GVDYRFVETGYVGTTLHLGPIYLPLRMLVPFVCSLALVGGLQLFLTRSFIGRAIMAVS
                     QDQLALQLMAANPVKIKRIAFALSIASAGIAGALLIIIQPVEPSIGRDYIGRVFAICV
                     LGGMGSFPGMVVAALLLGVVESITATFYGPSWAPAVAFGCLLLTLAFRPAGILGRRA"
     gene            825..1781
                     /locus_tag="VGP50_01175"
     CDS             825..1781
                     /locus_tag="VGP50_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8026005.1"
                     /translation="MSNGVFWAVAFAVAAAAFALSLAVENEFYIRAAFVVLQFLVLAT
                     AWNLLGGYTGYVNFGTAAFFALGAYGSVVMEKLAPLPIPALVLVGGVVSGAVGLGMGY
                     LTLRLRGAFFAIATLALAVVLQTLITNWDFVGGSRGAYIIAPAEIELLGHRIGYIRYL
                     FLVMLLLSVIAVATARSIERSRLGFGFAAIRDDEQAAEAAGVPTLRLKLIATTLSGAL
                     MGMAGAPYPFYITYLDPASGFNLNYAVNSIAMPLIGGMTSWVGPIVGALLIGTIQQFA
                     IVTSFLGASPAVNLLVVGVLLVAFVILAPRGIVGMMARHKGG"
     gene            1781..2518
                     /locus_tag="VGP50_01180"
     CDS             1781..2518
                     /locus_tag="VGP50_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8026006.1"
                     /translation="MAPPLLEVTGLGKRFGGFTALDAIDLAVGAGERVGLIGPNGSGK
                     STLVNCLCGTLRNESGSVRFDGHAIDGLAAHRRTRLGMARSFQLPKPFGSLTLLDNLR
                     IPLLYAVSARGRKHLTPADIERRSLELLDLVGLAGKARRLPRDLTQVEMRKLELVRAM
                     AAEPKLLIADEAMAGLSHSEVDDILALLFRLNERGITVILIEHIMRAVMSFSDRLVVL
                     VAGRKIADGLPREVVRNPEVERAYLGE"
     gene            2526..3239
                     /locus_tag="VGP50_01185"
     CDS             2526..3239
                     /locus_tag="VGP50_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8026007.1"
                     /translation="MSGLSAAYGAVRVLEDVALSVGSGETVALLGTNGNGKSTLIKCI
                     MGILRPTAGSIAAEIDGTRHELVGKTTEEIVDLGIALVPEGRRLFPKLTVEENLLLGA
                     FRRTARAHLTRNLAYCFEVFPVLAERRRQLAGSMSGGEQQMLALARALMSAPRILLVD
                     EPSVGLAPLLVRRTIEKIKELKDSYRLSVLMAEQNFTQAIRIADRGYVIVHGKIAFEG
                     RSADELNNNELIRKFYLGL"
     gene            3444..>3641
                     /locus_tag="VGP50_01190"
     CDS             3444..>3641
                     /locus_tag="VGP50_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobenzoate oxygenase"
                     /protein_id="HEV8026008.1"
                     /translation="MSTMYRYALPVEQTEWKFEGQVETRFTWEYDDERQSLLALYDKG
                     KKQQWDAALRIDWSQDLDPENP"
BASE COUNT          531 a         1282 c         1151 g          677 t
ORIGIN      
        1 ggcggcttct acgccgccat gacggtcggc gtgtcgatct ccttcggcat gctcgacatc
       61 gtcaacatcg cgcaccccgc tttcatcatc ctcggctcct acatcgccta catcgtcaac
      121 gcgcgcctcg gcatcgaccc cgtcgtcgcg agcattctcg cagcgccgct tttctatgcg
      181 ctgggcgcgc tcatctatca gatctaccac gtcgccttcg agcggcgggg cgacgaggcg
      241 ctgcgcggac tcgccttctt cttcgggttg ctcttcatca ccgaagtggc gctgatcctc
      301 gtgttcggcg tcgattatcg cttcgtcgag accggctatg tcggcaccac gcttcatctc
      361 gggccgatct atctgccctt gcgcatgctc gtccccttcg tctgctcgct ggcgctggtg
      421 ggcggcttgc agctctttct cacgcgcagc ttcatcggcc gcgccatcat ggcggtgtcg
      481 caggaccagc tcgcgctcca gctcatggcg gcgaacccgg tcaagatcaa gcgcatcgcc
      541 tttgcgctgt cgatcgccag cgccggcatc gccggcgccc tcctcatcat catccagccg
      601 gtcgagccgt cgatcggacg cgactatatc ggccgcgtct tcgccatctg cgtgctgggc
      661 ggcatgggca gctttcccgg catggtcgtc gccgctctgc tgctcggcgt cgtcgaaagc
      721 atcaccgcca ccttctacgg cccctcctgg gcgcccgccg tcgccttcgg ctgcctgctg
      781 ctcactctcg ccttccggcc cgccggcatc ctcggacgcc gcgcgtgagc aacggcgtct
      841 tctgggccgt cgccttcgcc gtcgccgccg cggcattcgc tctctcgctc gcggtcgaga
      901 acgagttcta tatccgcgcc gccttcgtcg tcctgcagtt tctcgtgctg gcgacggcgt
      961 ggaacctgct gggcggctac accggctatg tgaatttcgg cacggcggcg ttcttcgcgc
     1021 tgggcgccta tggctccgtc gtgatggaga agctggcacc gctgccgatc ccggcgctgg
     1081 tgctcgtggg cggcgtcgtg tcgggcgccg tcggcctcgg catgggctat ctgacgctgc
     1141 ggctgcgcgg cgctttcttc gccatcgcga cgctggcgct tgcggtcgtg ctgcagacgc
     1201 tcatcaccaa ctgggatttc gtcggcggct cgcgcggcgc ctatatcatc gcgccggcgg
     1261 agatcgagtt gctcggccac cgcatcggct atatccgcta tctgttcctc gtcatgctgc
     1321 tgctgtccgt catcgccgtc gcgacggcgc gcagcatcga gcgctcgcgc ctgggattcg
     1381 gcttcgcggc catccgcgac gacgagcagg cggccgaagc cgcgggcgtg ccgacactgc
     1441 gcctcaagct gattgcgacg acgctctcgg gcgcgctcat gggcatggcc ggggcgccct
     1501 atccttttta catcacctat ctcgacccgg cttccggctt caacctcaac tacgcggtca
     1561 acagcatcgc catgccgctg atcggcggga tgacgagctg ggtcggaccg atcgtcggcg
     1621 cgctgctgat cggcaccatt cagcaatttg ccatcgtcac gagcttcctc ggcgcgtcgc
     1681 cggccgtgaa cctgctggtg gtgggcgtgc tcctcgtcgc cttcgtgatc ctcgcgccgc
     1741 gcgggatcgt cgggatgatg gcccggcaca aggggggatg atggcgccgc cgctcctcga
     1801 ggtgaccggt ctgggcaaac gcttcggcgg cttcaccgcg ctcgatgcca tcgatctcgc
     1861 cgtgggcgca ggcgagcgcg tcggcctcat cggtcccaac ggctccggca agagcacgct
     1921 cgtcaactgc ctctgcggca cgctcaggaa cgagagcggc agcgtgcgct tcgacggcca
     1981 tgcgatcgac ggactggcgg cgcatcggcg cacgcggctc ggcatggcgc gcagcttcca
     2041 gctgccgaag cccttcggca gcctgacgct cctcgacaat ctgcgcatcc ccttgctcta
     2101 tgccgtgagc gcgcgcggtc gaaagcatct cacgccggcc gacatcgagc ggcgctcgct
     2161 cgagctcctc gacctcgtcg gcctcgccgg caaggcaagg cgcctgccgc gcgatctgac
     2221 ccaggtcgag atgcggaagc tcgagctggt gcgcgccatg gcggcggagc cgaagctgct
     2281 catcgccgac gaagcgatgg ccgggctgtc gcattccgag gtcgacgaca tcctggcact
     2341 gctgttccgc ctcaacgagc gcggcatcac ggtcatcctc atcgagcaca tcatgcgcgc
     2401 cgtgatgagc ttctccgatc gcctcgtggt cctggtcgca ggacgcaaga tcgccgacgg
     2461 cctgccgcgc gaggtcgtgc gcaatcccga ggtcgagagg gcctatcttg gcgagtagcc
     2521 tccgggtgag cggcctctct gcggcctatg gcgcggtccg cgtcctcgag gacgtggcgc
     2581 tgtccgtcgg cagcggcgag acggtggcgc tgctcggcac caatggcaac ggcaagagca
     2641 cgctcatcaa atgcatcatg ggcatcctgc ggccgaccgc cggcagcatc gcggcggaga
     2701 tcgacggcac gcgccatgag ctcgtgggca agaccacgga agagatcgtc gatctcggca
     2761 tcgccctcgt gcccgagggg cgccgccttt tcccgaagct gaccgtcgag gagaatctgt
     2821 tgctgggcgc gttccggcgg accgcgcgcg cccatctgac gcgcaatctc gcctactgct
     2881 tcgaggtctt cccggtgctt gccgagcgcc ggcgccagct cgccggcagc atgagcggcg
     2941 gagagcagca gatgctggcg ctggcccgcg cgctcatgtc ggcgccgcgc atcctcctgg
     3001 tggacgagcc ctcggtcggg ctggcgcctc tcctcgtgcg gcgcacgatc gagaagatca
     3061 aggagctcaa ggacagctat cgcctgagcg tgctcatggc cgagcagaac ttcacgcaag
     3121 ccatccggat cgccgaccgc ggctatgtca tcgtccatgg caagatcgcc ttcgaagggc
     3181 gctccgccga cgagctcaac aacaacgagc tgatccgcaa attctacctc ggtctttaac
     3241 ccatccgccg aaccgcgacg gtatcagcga aattacctgc aaatcagcga cttagccgcg
     3301 cccccgcgcg cccgcggtat cggcattcgg ccgctcgtca tcggacctgt tcacatctct
     3361 gcccatcccg cgtaggctgg cagcgggcac ggccttgagc aacgagaaga tcggccggcc
     3421 gataccctgg aggagtgtcg atcatgtcga ccatgtatcg ctatgcgttg cccgtcgagc
     3481 agaccgaatg gaaattcgag ggccaggtcg agacgcgctt cacctgggag tatgacgacg
     3541 agcgccaaag cctgctcgcg ctctacgaca agggcaagaa gcagcaatgg gacgcggccc
     3601 tgcggatcga ctggtcacag gatctcgatc ccgagaaccc g
//
LOCUS       DASYNS010000038         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2350302, whole genome shotgun sequence.
ACCESSION   DASYNS010000038 DASYNS010000000
VERSION     DASYNS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2350302"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VGP50_01195"
     CDS             <1..359
                     /locus_tag="VGP50_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEV8026009.1"
                     /translation="AEWNFRDQPPEFWNHYNTAYPPGVHLRIHPLLHWTELDVWRYVR
                     RENIPMVSLYFAQAGRRYRSLGCASCTGTVASEAVDADDIIAELESTKIAERAGRAQD
                     QEAEDAFEQLRAQGYM"
     gene            371..2239
                     /gene="cysC"
                     /locus_tag="VGP50_01200"
     CDS             371..2239
                     /gene="cysC"
                     /locus_tag="VGP50_01200"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEV8026010.1"
                     /translation="MIQSRAFSIVIAGHVDHGKSTLIGRLLHDTGSLPEGKVAALREA
                     SRRRGMLFEWSFVMDALKVERDQGITIDTTRMRFKSALRDYLIIDAPGHVEFLKNMLT
                     GAAAAEAAILVVDVVEGMGEQTRRHAFLLHLLGIREIVVAINKMDCVAYAQERFETVA
                     RAARTYLAGLDLEATAIIPISARQGDMIAHRSVPPSWYEGPTVLEALDALKGAGEDLD
                     QPLRLPVQDVYKFDERRVVVGRIEAGWLRVGDRLRFSPGERSARIASLETWNAQPMVT
                     AVAGQSIGLTLDEDIFVERGQVASAPEAAPLTARRLKLRIFHFGHAPLLPGDAVQLQI
                     GLAEHAAVIEAVERVVDVNSLTSHAAAAVQRNDVADVVVTSRSPIAVDDPAQGRHLAR
                     GILRRGYEVIAGCLLDAALDVRQPAVALRHVTPVSSALTPAERGAAWGHAGGVLWLTG
                     LSGAGKSTLAHALEKELFRRQWRAVLLDGDTLRQSLNADLGFSEHDRAENVRRIGAVA
                     RLLAESGMIAIVACIAPRAAHREAVRNELGSLYHEIYVKAPLAVCEQRDVKGLYAKAR
                     RGEIAGFTGLSDPYEPPQAPALEIGTDALTPAECLEHLVLYVEQALRPDNLHRLAS"
     gene            2323..2496
                     /locus_tag="VGP50_01205"
     CDS             2323..2496
                     /locus_tag="VGP50_01205"
                     /inference="COORDINATES: protein motif:HMM:NF021362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEV8026011.1"
                     /translation="MFKALSWRATGALDTFIISYLVTGRFVIAGSIVSVEVLTKIVLY
                     YLHERVWAYLPVD"
     gene            2556..2756
                     /locus_tag="VGP50_01210"
     CDS             2556..2756
                     /locus_tag="VGP50_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026012.1"
                     /translation="MGNRRAGIVSVDRLTTIGSDGMTAAVGNARHGVLAPHLKRDHVA
                     VASVNSPMQCMMKEICAQCWGS"
     gene            complement(2874..>3060)
                     /locus_tag="VGP50_01215"
     CDS             complement(2874..>3060)
                     /locus_tag="VGP50_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026013.1"
                     /translation="SKTGLPRGGQWRCLWLSKVDKAELRDGAWYSGGSHNQPQGCVEI
                     VDLDVNPASPYNPKRRL"
BASE COUNT          484 a         1025 c         1012 g          539 t
ORIGIN      
        1 atgcggagtg gaacttccgc gaccagccgc ccgagttctg gaaccactac aacaccgcct
       61 atccgccggg cgtgcatctg cgcatccatc cgctcctcca ttggaccgag ctcgatgtgt
      121 ggcgctatgt gcggcgcgag aacatcccga tggtgagcct ctacttcgcg caggccggcc
      181 ggcgctatcg ctctctaggc tgcgcgtcct gcactggcac ggtggcgagc gaagccgtcg
      241 atgccgacga tatcatcgcc gaactcgaat cgacgaagat cgccgagcgc gccggtcgcg
      301 cccaggacca ggaggcggag gacgctttcg agcagctgcg cgcccaaggc tatatgtgag
      361 gacgccgtcg atgatccaaa gccgcgcctt ctccatcgtc atcgccggcc atgtcgatca
      421 cggcaagtcg acgctgatcg gccgtctctt gcacgacacg ggttcgctcc cggagggcaa
      481 ggtggcagcg ctgcgggagg cgagccggcg gcgcggcatg ctgttcgaat ggtccttcgt
      541 catggatgcg ctcaaggtgg agcgcgacca ggggatcacc atcgacacga cgcgtatgcg
      601 tttcaagagc gccttgcgcg actatctgat catcgacgcg ccgggccatg tcgagttcct
      661 caagaacatg ctgacgggcg ccgccgcggc cgaggcggcg atcctggtgg tggacgtggt
      721 ggagggcatg ggcgagcaga cgcggcgtca cgcgttcctg ctgcatctcc tcggcattcg
      781 cgagatcgtc gtcgccatca acaagatgga ctgcgtcgcc tatgcgcagg agcgcttcga
      841 gaccgtggcg cgggcggcgc ggacgtatct ggcggggctc gatctcgagg cgacggcgat
      901 cattcccatc tcggcgcgcc agggcgacat gatcgcccat cgctcggtgc cgccgtcctg
      961 gtatgagggg ccgaccgtgc tcgaagccct cgacgcgctc aagggcgccg gcgaagacct
     1021 cgaccagccg ctgcgactcc cggtccagga cgtctataaa ttcgatgagc ggcgcgtcgt
     1081 ggtggggcgg atcgaggcgg ggtggctcag ggtcggcgac cgattgcgct tctcgccggg
     1141 cgagcgctcc gcgcgcatcg cctcgctcga gacgtggaac gcgcagccca tggtcaccgc
     1201 cgtcgccggg cagtcgatcg gcttgacgct cgacgaggac atcttcgtcg agcgcggtca
     1261 ggtcgcgagc gcgcccgagg cggcaccgct caccgcgcgg cggctgaagc tccggatctt
     1321 ccatttcggg catgcgccgc tcctccccgg cgatgctgtc cagctgcaaa tcggcctcgc
     1381 cgagcatgcc gccgtgatcg aggcggtgga gcgcgtcgtc gacgtgaaca gcctcaccag
     1441 tcacgccgcc gccgctgtcc agcgcaacga cgtcgccgat gtggtggtga cgagccgctc
     1501 gcccatcgcc gtcgacgatc cggcgcaagg ccggcatctc gcgcgcggca tcctgcgccg
     1561 cggctatgag gtcatcgccg gctgcctcct cgatgcggcg ctcgatgtcc gccagccggc
     1621 ggtggcgctt cgccatgtga cgccggtcag ctcggcgctg acgccggccg agcgtggcgc
     1681 cgcctggggc catgcgggcg gcgtgctctg gctcaccggg ctttcgggcg ccggtaagtc
     1741 gacgctggcg cacgccctcg agaaggagct gttccgccgc cagtggcgcg ccgtgctcct
     1801 cgatggcgac acgctgcgcc agtcactcaa tgccgatctc ggcttctccg agcatgatcg
     1861 cgccgagaat gtgcggcgca tcggcgccgt ggcacggctc ctggcggaga gcggcatgat
     1921 cgccatcgtc gcctgcatcg ccccgcgtgc agctcatcgc gaagcggtac gcaacgaact
     1981 cggttcgttg tatcatgaga tctatgtaaa ggcgccgctc gccgtctgcg agcagcgcga
     2041 tgtgaagggg ctctatgcca aggcgcggcg cggcgagatc gccggcttca cgggattgtc
     2101 cgatccctac gagccgccgc aagcgcccgc gttggagatc ggcaccgacg cgctaacccc
     2161 cgcggagtgc ctcgagcacc tcgtcctcta cgtcgaacaa gccctccgtc ccgacaatct
     2221 gcatcggctg gcatcctagg ctgcgaggct catcccatgc acgcattcac gcccgtagaa
     2281 aagcggcctt tacaacgtcg catgccctcg cgcttcaggt cgttgttcaa agcattgtcc
     2341 tggcgcgcta ccggcgcgct cgacaccttc atcatcagct atctcgtgac cggccgcttc
     2401 gtcatcgccg gctcgatcgt cagcgtcgaa gtcctgacga agatcgtcct ctactatctg
     2461 catgagcggg tgtgggccta tttgcccgtc gactgacggc tcgatgcgag aagatccatg
     2521 aattacgcgg tcactgacac tcgatcgatc gctgattggg aaaccgccgc gccggcattg
     2581 tttccgtcga ccgcctcacc accatcggct ccgacggcat gacggcggcg gtgggcaacg
     2641 cgcgccacgg cgtgctggcg ccccatctta agcgcgacca tgtcgccgtc gcctccgtca
     2701 actcgccgat gcaatgcatg atgaaggaga tctgcgcgca gtgttggggc tcgtgaagaa
     2761 ggcgtgtcgg aagaggaatg tcgatagggc ggggtcgagt tcggcctggt agccctatgc
     2821 ggctgcccag agcagcaatt tgagttggtt gcggttcccc gcaaccaatt tcttcaaagc
     2881 cggcgcttcg ggttgtaagg gctcgccgga ttgacgtcga ggtcgacgat ttcgacgcag
     2941 ccttgcggct ggttgtggct gccgcccgag taccaggcgc cgtcgcgaag ctcggccttg
     3001 tcgactttgg ataaccacag gcaacgccac tgcccgccgc gcgggagccc cgtcttgctt
//
LOCUS       DASYNS010000039        11567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5159389, whole genome shotgun sequence.
ACCESSION   DASYNS010000039 DASYNS010000000
VERSION     DASYNS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11567
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5159389"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..96)
                     /locus_tag="VGP50_01220"
     CDS             complement(<1..96)
                     /locus_tag="VGP50_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEV8026014.1"
                     /translation="MRAIPLDIGVIHFVGIGGIGMSGIAEILHNLG"
     gene            complement(93..1241)
                     /gene="murG"
                     /locus_tag="VGP50_01225"
     CDS             complement(93..1241)
                     /gene="murG"
                     /locus_tag="VGP50_01225"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEV8026015.1"
                     /translation="MSGPSLSGLVVLAAGGTGGHMFPAEALARELLGRGLHVALVTDR
                     RGQAFGERLPGVALHRIRVGRFGAGLMSKVIGVAELALGTLEASRLLRKLAPAAAVGF
                     GGYPSVPTMLAATRLGVPTLVHEQNAILGRANRLLASRVRRIATSFPKVGAIRPADRD
                     RVIETGNPVRPAIAAERGTTYVAPRNDGPFQILVLGGSQGARVLSEVIPAACAKLPET
                     LRQRLRLVQQARAEDIEAVRRAHAASGVASELATFFDDVPARLARAHLVISRSGASSV
                     SEIAMVGRPAILVPYLHAADDHQTANAQALAAAGGAWVIAQAAFTAEILADRLSTLMV
                     APEELARAAAAAHALGRPDAARALADLVIATMRGSNGATTSPLRENAA"
     gene            complement(1238..2374)
                     /locus_tag="VGP50_01230"
     CDS             complement(1238..2374)
                     /locus_tag="VGP50_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEV8026016.1"
                     /translation="MNFARTDQSAVAQWWWTVDRWTLVALAALIGFGALLVMAASPGV
                     AERIHAGGVGHVDTLFFVKRYFAVLPAALLTMFAVSLQNPRMVRRIAFAGFVVSLILL
                     AYTLIGGVEIKGARRWISLPGLSLQPSEFVKPTFAVVAAWLFAQYRLNGSIIAQWTAI
                     LLYVLVAALLIRQPDLGMAAVVSAVWFTQFFLAGLRLFWAAAGVAGGVAGLVGAYLTL
                     PHVASRIDRFLDPASGDSYQVERSMDAFMNGGLWGRGPGEGTVKGSLPDAHADFVFAV
                     AGEELGLVACLAIVALFAFIVLRGFSRLLQENNLFVVLAATGLLVQFGLQAIINMASS
                     LELMPTKGMTLPFLSYGGSSLLALGLGMGIALALTRRRYGGVET"
     gene            complement(2371..3756)
                     /gene="murD"
                     /locus_tag="VGP50_01235"
     CDS             complement(2371..3756)
                     /gene="murD"
                     /locus_tag="VGP50_01235"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV8026017.1"
                     /translation="MIEVAAFRGEQVAVMGLARSGLVAARALQRGGARVLAWDDAPAK
                     REEAAAQGVPIADLAQADLAGTKALVLSPGIPHTFPKPHPVAARARAAGASIIGDIEL
                     LARSCQLAHYVGVTGTNGKSTTTALMGHILAKAGRDVAVGGNIGTPVLTLAALGADGV
                     YVLEMSSYQLELTETLTFDVAVLLNITPDHLDRHGGMEGYIAAKRRIFARQQKPQAAV
                     IGVDDALGRAMADTLMAEGAQRVVPISAETRVLRGIYVEGGTLIDEMGGAPARVLDLA
                     RAQRLPGRHNWQNAAAAYAVARCLDIEVSAITEAITSFPGLAHRQELIATIDGVAYVN
                     DSKATNADAVAKALACYEDVYWIAGGVPKEGGIASLAPLFPRIRHAFLIGKASAEFAA
                     TLDGRVPTTRAGTLAEALRAAHAMAATEKQQSAVVLLSPACASFDQFTNFEERGDAFR
                     RLVEALPGARP"
     gene            complement(3763..4869)
                     /gene="mraY"
                     /locus_tag="VGP50_01240"
     CDS             complement(3763..4869)
                     /gene="mraY"
                     /locus_tag="VGP50_01240"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV8026018.1"
                     /translation="MLYHLLFPLADQFGPFNLFRYLTFRSGGALVTALLISFLMGPRV
                     IAWLKSKQAEGQPIRLDGPEGHLIRKKGTPTMGGFLILLALIVSTLLWADLTDRYVWV
                     VLFVTVAFGAIGFFDDYKKLTKRSSSGLSGRKKLLAEVAVAVLAALAVMWLTRGPAIT
                     PPWLGSALAVPFLKNVLFDLGWFFVVFAVFVIAGASNSVNLTDGLDGLAIVPAMIAAG
                     CFALISYLAGNRVFADYLQIHHVPGAGELAVFCSALIGAGLGFLWFNAPPAMVFMGDT
                     GSLSIGAALGTISVVTKHELVLAIIGGLFVLETVSVIVQVASFKLTGRRVFRMAPLHH
                     HFEKQGWQEPTIVIRFWIIASILAIAGLSTLKLR"
     gene            complement(4945..6360)
                     /locus_tag="VGP50_01245"
     CDS             complement(4945..6360)
                     /locus_tag="VGP50_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HEV8026019.1"
                     /translation="MTALWTANEAAAATGGRSNRDWSAEGVSIDSRSVGPGDLFIALT
                     GPNFDGHDFVADALRKGAAAALVARVPEGVAADAALLVVADTMAALEDLGRAGRRRGS
                     AAVIGVTGSVGKTGTKEALKRALERQGLSFASAGSLNNQWGVPLSLARMPRDTAYGIF
                     EMGMNHPGEIAALSQIVRPDVAVITTIEPAHLGFFPSVEAIADAKAEIFLGMEPGGAA
                     VLNRDNPHFARLAAAARARGISRIIGFGSDAEAAVKLVDCQLYASASAVTASVMGEVV
                     DYCIAIPGRHWVMNSLAVLGAVKAAGGDVDAAAAAMSSLAPLDGRGRRHSIAVTGGTA
                     ELIDESYNASPASMRAAFAVLAGTEPGKGGRRIAALGDMLELGNDARDLHAALAQPLV
                     DARIDLVFTAGAQMQALHDALPKRMRGGHAATGAALADSLVQRLRPGDIITVKGSHGS
                     RMGEVVKHLLAGARVPDAVKG"
     gene            complement(6357..7826)
                     /locus_tag="VGP50_01250"
     CDS             complement(6357..7826)
                     /locus_tag="VGP50_01250"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV8026020.1"
                     /translation="MDGAQANLLPTPAAATEIRGLTADSRDVRPGYLFAALAGSRADG
                     RRFVDDAVARGAVAVLTDDAARVGALEQRSPPVRVIVDANPRRRLARMAARFYAPQPE
                     TLVAITGTNGKTSVTVFTRQIWQKLGARAASLGTIGIVGPGFANPGALTTPDPVTLHR
                     ELQLLARAGIDHAALEASSHGLDQYRLDGLDLRAAAFTNLTRDHLDYHHDMDAYFAAK
                     TRLFTEVLPDDGTAVLNADVPEGRRLAKLCHERGQTVLTFGTAADADLRLISAEPLEA
                     GQRLHLKALGKERALFLPLIGAFQASNVLAALGLAIATGAAVEQALDALPTLEGVPGR
                     LEHVATHPDGAPVIVDYAHTPDALETVLLALRGHCRGRLVVVFGCGGDRDAGKRPQMG
                     AIAERLAEIVIVTDDNPRSEDPAAIRRAILAQCPKAAEIGDRAAAIRRAVQLLEPGDL
                     LLLAGKGHERGQIVADVTLPFEDAAVARAAVAELPRARA"
     gene            complement(7831..9570)
                     /locus_tag="VGP50_01255"
     CDS             complement(7831..9570)
                     /locus_tag="VGP50_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEV8026021.1"
                     /translation="MMSRHDDNPCRPRHFKPAPCAPAALEGPAQQSLETSHTRLLVTA
                     ALFCLAFLLIALRLVEVTAIKSEAYAARSPVPTQPLVSRADIVDRNGVLLATTLSTPS
                     LYANPKLVKDPAAVARRLVGVLPDLVESEVEAKLASDKSFVWIKRQLTPHQELAVNRL
                     GVPGLQFEPEERRFYPEGSLAAHIVGYTGIDNRGLGGIERGFDDILRDRRDAVALSID
                     ARVQYILRDEIARQIADFNASGGMGVIMDVNTGEVLAMVSLPDFDPNNAGSATPETVF
                     NRATLGIYEMGSTFKIFNTAMALEARTATLASSYDATKPLHVGRFTIHDDHPQNRWLN
                     VAEIFQYSSNIGAARMAVEAGTDRQRDFLGRLGLLRAPGFELPEIGAPLVPSPWRDVN
                     TMTVAFGHGISVSPLQMAIAVSAVVNGGVLHPATLIKRAPEYAPPGRQVLSARTSDDM
                     RRLLRLVVEHGTGKLAIAPGYLLGGKTGTAEKVNGARYDKKGLLSSFIGVFPITEPRY
                     LVMISIDEPHPNKASSGFATGGWAAAPGVGRVVQRMAPLMGIPPVDEDSPEIRRSLMV
                     DSARPQGPSFAAN"
     gene            complement(9567..9944)
                     /locus_tag="VGP50_01260"
     CDS             complement(9567..9944)
                     /locus_tag="VGP50_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026022.1"
                     /translation="MIRLATIAWILLVAASGYAMFQVKYEVATLDQQLAQLDRGIAQS
                     REATRVLSAEWSLLNDPRRLDHLARRHLDLHPLLPAQLASIDSLPRRSQTPPAAVAVA
                     PSPVASLPAAASRLASIKPVVTP"
     gene            complement(9941..10906)
                     /gene="rsmH"
                     /locus_tag="VGP50_01265"
     CDS             complement(9941..10906)
                     /gene="rsmH"
                     /locus_tag="VGP50_01265"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV8026023.1"
                     /translation="MTAAPAHTPVLLDEVVAALAPRDQALYVDGTFGGGGYARALLAA
                     ARCPVIGIDRDPSAIAAGAALAQEHPDRLTLIEGRFGEMAALLAPLGIGPGGVSGGIV
                     LDLGVSSPQLDDPRRGFSFRADGPLDMRMGSSGSTAADLVNTLPEAALSKLIYVNGEE
                     RFSRRVARAIAAARQKAPLTRTTELAELVRAVVPAGDGIDPATRTFQALRIEVNDELG
                     ELERALHAAERLLAAGGRLAIVSFHSLEDRRVKDFLRERSPTAPRGSRHRPESPPRPP
                     SFRLLTRKPVTSSAAEIARNPRARSARLRAAERTSAPPWASSEAA"
     gene            complement(10903..11379)
                     /gene="mraZ"
                     /locus_tag="VGP50_01270"
     CDS             complement(10903..11379)
                     /gene="mraZ"
                     /locus_tag="VGP50_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEV8026024.1"
                     /translation="MGLFFSTVVNKVDRKGRVSVPATFRAALEGLSFPGVILFRSFRL
                     SALDGSGIDRMEELSARIDALDEFSEDREALASIFADAQQLPFDGEGRIVLPEALSAH
                     AGITENAAFVGLGRTFQIWEPQRFEKHQQDLRERARRQGTTLPSRRPDSPPPERKP"
BASE COUNT         1953 a         3778 c         4185 g         1651 t
ORIGIN      
        1 tccgagattg tgcaggatct cggcgatacc gctcatgccg atgccgccaa tgccgacgaa
       61 atggatgacg ccgatgtcga gcggtatggc tctcatgcgg cgttctcccg aaggggagac
      121 gttgtagcgc cgttgctgcc gcgcatcgtc gcgatcacga gatcggcaag agcgcgggcc
      181 gcatcgggcc gcccgagcgc gtgagctgcc gcggccgcgc gggcgagctc ttcgggcgca
      241 accatcagcg tgctgaggcg atcggcgagg atttcggccg tgaaggccgc ttgggcaatc
      301 acccaggcgc cgcccgccgc cgcgagtgcc tgggcattgg cggtctgatg atcgtcggca
      361 gcgtggagat aggggacaag gatcgccggc cggccgacca tggcgatctc ggacacgctc
      421 gatgcgcctg agcgcgaaat cacgagatgg gcgcgggcga gccgcgccgg cacatcgtcg
      481 aagaaagtgg cgagctcgga ggcgacgccg ctcgccgcat gggcacggcg caccgcctcg
      541 atgtcctcgg cgcgcgcttg ctgcacgagg cggaggcgct ggcgcagcgt ttccgggagc
      601 ttggcgcaag ccgccgggat gacctcgctc agcacgcgcg cgccctggct gccgccaagg
      661 acgaggatct ggaaaggacc atcgtttcgc ggcgcgacat aagtcgtgcc gcgctcggcg
      721 gcgatcgccg gacgcacggg attgcccgtt tcgatgacgc gatcgcggtc ggcgggcctg
      781 atggcgccaa ctttcgggaa agacgtcgcg atgcgccgga cgcgcgacgc gagcagccgg
      841 ttggcgcggc cgagaatcgc gttctgctcg tggaccagcg tcggaacccc cagccgcgtt
      901 gccgcaagca tggtcggcac cgaggggtag ccgccgaagc ccactgctgc ggcgggcgcg
      961 agcttccgca gcaggcggct tgcctcgagc gtgcccaacg ccagctcggc gacgccgatg
     1021 accttgctca tgagtccggc gccgaagcgg ccgacgcgaa tccggtggag cgccaccccg
     1081 ggcagccgct cgccgaacgc ttgcccccgc cggtcggtga caagcgccac atggagaccc
     1141 cggcccagca gctctcgcgc caacgcctcc gccggaaaca tgtgtccgcc ggtgccgccg
     1201 gcggcaagca cgacgagccc ggacagcgac ggcccgctca tgtctcgact ccgccatagc
     1261 gccgccgcgt cagcgccagc gctataccca tgccgaggcc gagcgccagg agcgaggagc
     1321 cgccatagga caggaacggc agcgtcatgc ccttggtcgg catgagctcg aggctcgatg
     1381 ccatgttgat gatcgcctgc agcccgaact ggacgagaag tcccgtcgca gcgagcacga
     1441 cgaagagatt gttctcttgc agcagccgcg agaagccgcg cagcacgatg aaggcgaaga
     1501 gagcgacgat ggcgaggcag gcgacgagcc cgagttcttc gccggcaacg gcgaagacga
     1561 aatccgcatg cgcgtcgggg agcgatccct tgaccgtgcc ttcgcccgga ccgcggcccc
     1621 agaggccgcc attcatgaag gcgtccatcg atcgctcgac ctgatagctg tcgccgctcg
     1681 cgggatcgag aaagcggtcg atccggctcg ccacatgcgg caacgtcaga taggcgccga
     1741 caaggccggc gacgcctccc gccacgccgg cggccgccca gaagagccgc aggcccgcga
     1801 ggaagaactg tgtgaaccac accgccgaca ccaccgccgc cataccgagg tcgggctggc
     1861 gaatgagcag cgccgccacc aggacataga gcagaatggc ggtccattgc gcgatgatgc
     1921 tgccgttcag acggtattgc gcgaagagcc aggcggcgac cacggcgaag gtgggcttga
     1981 cgaattctga aggctgcaag gacaagccgg gaaggctgat ccagcgccgc gcgcctttga
     2041 tctcgacacc gccgatgagg gtgtaagcga gaaggatcag cgacaccacg aacccggcga
     2101 acgcgatgcg ccgcaccatg cgcggattct gcagcgacac tgcgaacatc gtcaggaggg
     2161 cggcgggcag cacggcgaaa tagcgtttca cgaagaagag cgtatcgaca tggccgacgc
     2221 cgcccgcatg gatgcgctcg gccacaccgg ggctcgccgc catgacgaga agggcgccga
     2281 agccgatcaa cgcggcaagc gccaccagcg tccagcgatc gacggtccac caccattgcg
     2341 ccaccgcgct ttgatcggtg cgggcgaaat tcatggccgc gctcccggca gcgcctcgac
     2401 cagccggcga aacgcgtcgc cgcgctcttc gaaattggtg aactggtcga aggacgcgca
     2461 ggccggcgag agcagtacga cggcgctttg ctgcttctcc gtcgccgcca tcgcatgagc
     2521 tgcgcgcagc gcctcggcca gcgtgccggc gcgcgtcgtc ggaacgcggc catcgagcgt
     2581 cgctgcgaat tccgcgctcg ccttgccgat gaggaaggcg tggcggatgc gcgggaagag
     2641 cggcgccaaa gaggcgatgc caccttcctt gggaacgccg ccggcgatcc agtagacgtc
     2701 ttcgtagcag gcgagcgcct tggcaacggc gtccgcattg gtggccttgg agtcgttgac
     2761 ataggcgacg ccatcgatgg tggcgatcag ctcctggcgg tgagcgaggc ccgggaagct
     2821 cgtgatcgcc tcggtgatcg ccgacacttc gatgtcgagg cagcgcgcga ccgcataggc
     2881 cgcggcggcg ttctgccaat tgtgccggcc gggaagccgc tgcgcgcggg cgagatcgag
     2941 gacgcgggct ggtgcgccgc ccatctcgtc gatgagcgtg ccgccttcga catagatgcc
     3001 gcgcaggacg cgcgtctcgg ccgagatcgg gacgacgcgc tgcgcgcctt ctgccataag
     3061 cgtgtctgcc atggcgcggc ccagcgcatc gtcgacgccg atcactgcgg cctgcggctt
     3121 ctgttggcgc gcaaagatgc ggcgcttggc ggcgatgtag ccttccatgc cgccatggcg
     3181 gtcgagatgg tcgggcgtga tgttgagcaa caccgcgacg tcgaaggtca gcgtctccgt
     3241 gagctccagc tgataggagc tcatctccag cacatagact ccgtccgcgc cgagtgccgc
     3301 cagcgtcagc accggcgtgc cgatattgcc gcccactgcc acgtcgcggc cggcctttgc
     3361 gaggatgtgg cccatgagcg cggtggtcgt cgatttgccg ttggtgccgg tgacgccgac
     3421 ataatgcgcg agctggcagc tgcgcgcgag aagctcgatg tcgccgatga tcgacgcgcc
     3481 ggccgcccgg gcgcgcgccg cgacgggatg cggcttcgga aaggtgtgcg gaataccggg
     3541 cgagaggacg agcgccttgg tgccggcgag atcagcttgc gcgagatcgg cgatcggcac
     3601 gccctgagcc gccgcttcct cgcgctttgc cggagcatcg tcccatgcca gcacgcgggc
     3661 gccgccgcgc tgcagcgccc gagcggcgac gaggccggag cgcgccagcc ccatcaccgc
     3721 cacctgctcg cctcggaacg ccgccacctc gatcatccct ccctaccgca gcttcaacgt
     3781 cgagagcccg gcgatggcca ggatggaagc gatgatccag aagcggatga cgatggtcgg
     3841 ctcctgccat ccctgcttct cgaaatggtg gtggagcggc gccatgcgaa agacgcgcct
     3901 gccggtgagc ttgaagctcg cgacctggac gatgacggac accgtctcca ggacaaagag
     3961 cccgccgatg atggcgagca cgagttcatg cttggtcacg acgctgatgg tgcccaacgc
     4021 ggcgccgatc gaaagcgagc cggtgtctcc catgaagacc atggcgggcg gcgcgttgaa
     4081 ccagaggaag ccgagccccg ctccgatcag cgccgagcag aacaccgcaa gctcgccggc
     4141 gccggggacg tgatggatct gcagatagtc ggcaaacact ctgttgcccg cgagatagga
     4201 gatgagcgca aaacatccgg cggcgatcat cgccgggacg atggcgaggc cgtcgagccc
     4261 gtcggtgagg ttcacggagt tggacgcgcc ggcgatgacg aacaccgcga acacgacgaa
     4321 gaaccagccg agatcgaaca gcacattctt cagaaaaggc acggcgagcg cgctgccgag
     4381 ccagggcggc gtgatcgcgg gaccgcgcgt cagccacatg acggcgagag cggcaagcac
     4441 cgcgacggcg acctctgcca gcagcttctt gcggcccgag aggccggagg aggagcgctt
     4501 cgtcagtttc ttgtagtcgt cgaagaaacc gatggcgccg aaagcgaccg tgacgaagag
     4561 gaccacccag acatagcggt ccgtgagatc ggcccagagc agcgtcgaga cgatgagtgc
     4621 cagcaggatg aggaagccgc ccatggtggg cgtgcccttc ttgcgtatga gatggccctc
     4681 ggggccgtcg agacggatcg gctgtccctc cgcctgcttc gatttgagcc aggcaatcac
     4741 gcgcggcccc atcaggaagc tgatgagcag cgccgtcacc agcgcgccgc ccgagcggaa
     4801 ggtcagatag cggaagagat tgaacggccc gaactgatcg gcgagcggaa agagcaggtg
     4861 atacagcatc ggctaatccg caccccccac cctgaccttt cccctcgaac ggggaaggga
     4921 aaatatgagc cagccgctca tctctcagcc cttcaccgcg tccggcaccc gcgcgccggc
     4981 gagcaggtgc ttcaccacct cgcccatgcg gctgccatgc gagcccttga ccgtgatgat
     5041 gtcgccggga cgcaggcgct ggacgagact gtcggccagt gccgcccccg tcgcggcgtg
     5101 gccgccgcgc atgcgcttcg gcagcgcatc gtggagcgct tgcatttgcg cccctgccgt
     5161 gaagacgaga tcgatccgtg catcgaccag cggctgggcg agcgccgcat gaagatcccg
     5221 cgcatcgttg cccagctcga gcatgtcgcc gagcgcggca atacggcggc cgcctttgcc
     5281 gggttcggta ccggcgagca cggcgaaggc ggcgcgcatc gaggcggggc tcgcattgta
     5341 gctctcgtcg atgagctcgg cggtgccgcc ggtgacggcg atactgtggc ggcggccgcg
     5401 accgtcgagc ggcgccagcg acgacatcgc ggctgcggcg gcatcgacat cgccgcccgc
     5461 cgccttgacc gcgcccagca cggctaggct gttcatcacc caatggcggc cgggaatcgc
     5521 gatgcagtag tcgacgacct cgcccatcac cgaggccgtc accgcgcttg cgctggcata
     5581 gagttggcaa tcgacgagct tcaccgccgc ctcggcatcg ctgccgaagc cgatgatgcg
     5641 gctgatgccg cgcgcgcgcg ctgcggcggc gagccgcgcg aaatggggat tgtcgcggtt
     5701 gagcacggcc gccccgcccg gctccatgcc gaggaagatc tcggctttgg catcggcgat
     5761 cgcctcgacc gagggaaaga atccaagatg cgccggctcg atcgtggtga tcaccgcgac
     5821 gtccgggcgc acgatctggc tcaacgccgc gatctcgccc ggatggttca tgcccatctc
     5881 gaaaatgcca taggccgtat cgcgcggcat gcgcgcgagc gacagcggca cgccccactg
     5941 attgttgagg ctgcccgccg acgcgaagct cagaccttgg cgctccaagg cgcgcttcag
     6001 cgcctccttg gtcccggtct tgccgacgct cccggtgacg ccgatgacgg ccgcgctgcc
     6061 gcgccggcgg cccgctcggc ccagatcctc gagcgccgcc atggtatcgg cgacgacgag
     6121 cagcgccgca tcggccgcca caccctccgg cacgcgcgcg acgagagcgg ctgcggcgcc
     6181 cttcctgagc gcgtcggcga cgaagtcatg gccgtcgaaa ttcggtcccg tcagcgcgat
     6241 gaagaggtcg cccggaccaa ccgaacggct gtcgatcgag acaccctcgg cgctccagtc
     6301 gcgattgctg cggccgcccg tggccgcggc cgcttcgttc gccgtccaga gcgcggtcat
     6361 gcgcgtgccc gcggcagctc ggcgacagcc gcgcgcgcga cggccgcatc ctcgaagggc
     6421 agggtgacgt cggcgacgat ctggccgcgc tcatgtcctt tgccggcgag caacagcaga
     6481 tcgcccggct cgagcaattg gacggcgcgc cgaatggcag cggcgcggtc gccgatctcc
     6541 gcggccttgg ggcattgcgc gagaatggcg cgacggatcg cggcgggatc ctcgctgcgc
     6601 ggattgtcat cggtgacgat gacgatttcg gcgagacgct cggcgatggc gcccatttgc
     6661 ggccgcttac cggcgtcgcg gtcgccgccg cagccgaaca ccacgaccag ccggccgcgg
     6721 cagtggccac gcagtgccag aagcacggtc tcgagggcat cgggcgtgtg cgcatagtcg
     6781 acgatgaccg gcgcaccgtc gggatgtgtc gccacatgct cgaggcggcc gggcacgcct
     6841 tcgagcgtcg gtagcgcgtc aagcgcctgc tcgaccgccg cgcccgtggc gatggcgagg
     6901 ccgagagcgg ccagcacgtt gctcgcctgg aacgcgccga tcagcggcag gaagagcgct
     6961 cgctccttgc cgagcgcctt gagatgcaag cgctgcccgg cctcgagcgg ctcggccgag
     7021 atcaggcgca gatcggcatc ggcggcggtg ccgaaggtga gcacggtctg gccgcgctca
     7081 tgacagagct tggcgaggcg gcggccttca ggcacatccg cgttgagcac cgcggtcccg
     7141 tcatccggca ggacttcggt gaagagccgc gtcttggcgg cgaaatacgc gtccatgtcg
     7201 tggtgataat cgagatggtc gcgtgtaaga ttggtaaacg cggcggcgcg gaggtcgaga
     7261 ccgtcgagcc gatattggtc gaggccatgg ctcgatgcct cgagcgcggc atggtcgatg
     7321 ccggcgcgcg cgagaagctg cagctcgcgg tggagcgtga ccggatcggg cgtggtgagc
     7381 gcgcccggat tggcgaagcc cggcccgacg atgccgatgg tgccgaggct cgccgcgcgc
     7441 gcgcccagtt tctgccagat ctgccgcgtg aagaccgtga ccgaggtctt gccgttggtg
     7501 ccggtgatgg cgaccagcgt ctccggctgc ggcgcataga agcgggccgc catgcgggcg
     7561 agccgccggc gcgggttggc gtcgacgatg acgcgcacgg gaggcgagcg ctgctcgagc
     7621 gcgccgacgc gcgccgcatc gtcggtgagc acggcgacgg cgccgcgcgc caccgcgtca
     7681 tcgacgaagc ggcggccgtc ggcacggctc ccggcaagcg ccgcaaagag gtaccccgga
     7741 cgcacgtcgc gcgaatccgc ggtcaggccg cggatctcgg tggcggcggc gggcgtcggg
     7801 aggagattgg cttgagcgcc gtccatgagg tcaattagcc gcaaaactcg gcccctgcgg
     7861 gcgcgcggaa tcgaccatca aagaacggcg aatttctggc gaatcttcgt cgaccggtgg
     7921 aattcccatg agcggcgcca tgcgttgcac gacccggccc accccaggag ccgcagccca
     7981 ccccccggta gcaaacccag agctggcctt atttgggtgg ggctcgtcaa tgctgatcat
     8041 cacgagatag cgcggctcgg tgatggggaa gacgccgatg aaagaggaca agagcccttt
     8101 tttgtcataa cgggcgccgt tcaccttctc ggcggtcccg gtcttgccgc ccagcaggta
     8161 gccgggcgcg atggcgagct tgcccgtgcc gtgctcgacc acgaggcgca gcaggcggcg
     8221 catgtcgtcg gaggtgcggg ccgagagcac ctgccggccc ggcggcgcat actcgggcgc
     8281 gcgcttgatc agcgtcgccg gatggagcac gccaccattc accaccgcgc tcacggcgat
     8341 cgccatctgc agcgggctca ccgagatgcc gtggccgaag gcgaccgtca tcgtgttgac
     8401 gtcgcgccaa ggtgagggca cgagcggggc gccgatctcc ggcagctcga agccgggcgc
     8461 ccggaggagg cccagccgcc cgaggaagtc gcgctgccgg tcggtgccgg cctcgaccgc
     8521 catgcgtgcg gcgccgatat tcgacgaata ttggaaaatc tcggccacgt tgagccagcg
     8581 attctgcggg tggtcgtcgt ggatcgtaaa gcggccgaca tgcaaaggct tggtcgcgtc
     8641 gtagctgctg gccagagtgg cggtgcgcgc ctcgagcgcc atggcggtgt taaagatctt
     8701 gaacgtcgag cccatctcgt agatgccaag cgtggcgcgg ttgaagaccg tctcgggcgt
     8761 ggcgctgccg gcattgttcg ggtcgaaatc cggcagcgat accatggcga gcacttcgcc
     8821 ggtgttcacg tccatgatca cgcccatgcc gccgctcgcg ttaaaatcgg cgatttgccg
     8881 cgcgatctcg tcgcgcagga tgtattggac ccgggcgtcg atggagagcg ccaccgcatc
     8941 gcggcggtcg cgcaggatgt cgtcgaagcc gcgctcgatc ccgcccaggc cgcggttgtc
     9001 gatgccggtg tagccgacga tgtgggcggc gaggctgccc tcggggtaga agcggcgctc
     9061 ctcgggctcg aactggaggc cggggacgcc gagccggttc accgccagct cctgatgcgg
     9121 cgtcagctgc cgcttgatcc agacgaagct cttgtcggag gcgagcttgg cttcgacctc
     9181 gctctcgacg aggtcgggca acacgccgac gagacggcgg gcgacggcgg cggggtcttt
     9241 gaccagcttg ggattggcat agagggaagg ggtcgagagc gtggtggcga gcagcacgcc
     9301 gttccggtcg acgatgtcgg cgcggctcac gaggggctgg gtggggacgg ggctgcgcgc
     9361 ggcgtaggcc tcgctcttga tcgccgtcac ttcgacgagg cggagcgcga tgagcaggaa
     9421 ggcgaggcag aagagcgcgg cagtgacgag gagccgggta tgactggtct cgagcgattg
     9481 ctgcgccggc ccctcgagcg cggcgggagc gcagggcgcg ggcttgaaat gcctggggcg
     9541 gcaggggttg tcgtcgtgtc gcgacatcat ggcgtgacca ccggcttgat gctggctagc
     9601 cgcgacgccg ccgccggtaa ggaggctacc ggcgacggcg caacggccac cgcggcggga
     9661 ggcgtttggg aacgccgcgg taggctgtcg atggaagcga gctgggcagg caggagggga
     9721 tggaggtcga gatggcgacg ggcgagatga tcgagccggc gcggatcgtt gagaaggctc
     9781 cactcggcgc tcagcacgcg cgtcgcctcg cggctctgcg caatgccgcg atcgagctgc
     9841 gccagctgct ggtcgagcgt agccacctcg tatttgacct ggaacatggc atagccgctc
     9901 gcagcgacga gcaggatcca ggcgatggtc gcgaggcgga tcatgcggcc tccgagctcg
     9961 cccagggcgg ggcggatgtc cgctcggccg cgcgcagacg ggcggagcgg gcgcggggat
    10021 tgcgcgcgat ctcggcggcg cttgacgtta cgggcttgcg ggtgagcagg cggaagctgg
    10081 gcggccgcgg cggcgattcg gggcggtggc gggagccgcg cggtgccgtc gggctgcgct
    10141 cgcgcaggaa gtccttgacc cggcggtcct cgagcgaatg gaaggagacg atggcgagcc
    10201 gcccgccggc ggccagcagg cgctcggcgg catggagcgc gcgctcgagc tcgccgagct
    10261 cgtcattcac ctcgatgcgc aaggcctgga aggtacgggt ggcagggtcg atgccgtcgc
    10321 ccgccggcac gaccgcccgc accagctcgg cgagctcagt ggtgcgggtg agcggcgcct
    10381 tctgccgcgc cgcggcgatg gcgcgcgcga cgcggcgtga aaaacgctcc tcgccattga
    10441 cgtagatgag tttggacaac gccgcttcgg gcaaggtgtt gacgagatcg gccgcggtgc
    10501 tgccgctgct tcccatgcgc atgtcgagag ggccgtcggc gcggaaagag aagccgcggc
    10561 gcgggtcgtc gagctgcggc gaggagacgc cgagatcgag cacgatgccg ccgctcaccc
    10621 cgcccggtcc gatgccgagc ggcgccagca aggcggccat ctcgccgaag cggccttcga
    10681 tcagcgtgag ccggtccgga tgctcctgcg cgagagcggc gcccgcggcg atggccgagg
    10741 ggtcgcggtc gatgccgatg acggggcagc gcgcggccgc gagcagggca cgggcgtagc
    10801 cgccgccgcc gaaggtgccg tcgacataga gggcctggtc gcggggtgcg agcgcggcga
    10861 cgacctcgtc gagcaggacg ggggtgtggg cgggcgcggc ggtcatggct tgcgctccgg
    10921 cggcggcgag tcggggcgcc gcgagggcag cgtcgtgccc tggcgccggg cgcgctcgcg
    10981 cagatcctgc tgatgcttct cgaaacgctg cggctcccag atctggaagg tgcggccgag
    11041 cccgacgaaa gcggcattct ccgtgatccc ggcatgggcg gagagcgcct ccggcaggac
    11101 gatgcggccc tcgccgtcga agggcagctg ctgggcgtcg gcgaagatcg aggcgagcgc
    11161 ctcgcggtcc tcggagaatt cgtcgagcgc gtcgatgcga gcggagagct cctccatccg
    11221 gtcgatgccg ctgccgtcga gcgccgacag gcggaaggag cggaagagga tcaccccggg
    11281 aaagctcaag ccctcgagcg cggcacggaa ggtcgcaggc acggagacgc ggcccttgcg
    11341 gtccaccttg ttgacgactg tggaaaaaaa cagccccaaa gcacgtcccc ggccaactga
    11401 tcacaatacc cgccgcctta ccccgaattg ggatttcatg ggataacatg ggtaaaactg
    11461 gtcgtcaatg gaaaatgcca gtaaaacctt gggatcaaag gtattaatta tggttgaccg
    11521 aggaatcgcg cccctatggc caaagaagaa gctgttcctc tacgggt
//
LOCUS       DASYNS010000040         5013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5159542, whole genome shotgun sequence.
ACCESSION   DASYNS010000040 DASYNS010000000
VERSION     DASYNS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5159542"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VGP50_01275"
     CDS             complement(<1..321)
                     /locus_tag="VGP50_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV8026025.1"
                     /translation="MATAATAGPALAARKDNKDPAPRAAAVTAAPAGRPVIDTSARNA
                     FAIDFQTGAVLLDKGGDERIPTASMSKIMTAYVAYTYLAEGRAKLDDMLPVSEHAWRT
                     GLGGD"
     gene            complement(459..1388)
                     /locus_tag="VGP50_01280"
     CDS             complement(459..1388)
                     /locus_tag="VGP50_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEV8026026.1"
                     /translation="MTRRSASFLLLATALLAACASHAPPAAPTAPRAGEAAGRGTYKI
                     GNPYQIGGVWYAPAEDFSYDETGIASWYGEDFHGKYTANGEIFDLNALTAAHRTLPMP
                     SIVQITNLDNGRSMALRVNDRGPYARGRIVDVSRRAAQLLGFEAQGTAKVRVKILVPE
                     SLQVAALAGRNGGDVVAKASDDVPQSAPREPVLTEPLAAAPPSLRIASTEAPSLAPAA
                     PAPVLSGKVTVVPVGATQIFIQVGAYAVAANALRVKDRLDAIGPVKVYGARVKGVDLY
                     RVRLGPIGSVDQADQMLGRVVDAGLTEARIVVD"
     gene            complement(1443..2405)
                     /locus_tag="VGP50_01285"
     CDS             complement(1443..2405)
                     /locus_tag="VGP50_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEV8026027.1"
                     /translation="MLARRIVLAVVLLLAAGPASAEDADFAAWLEALKQEALAAGIQP
                     ATVARALDGIEPIPRVIELDRRQPEKVRTYEDYLEHVVSADRQRAAREQLARNRALLD
                     AVGQRYGVQPRFIVALWGIETSYGADPGRFPVIGALATLAYEGRRGPFFRAELVNALR
                     IVDQDGIDPAELQGSWAGAMGQAQFMPSTFLTYAVSYSGDGKRDIWHRREDVFASMAN
                     YLAQSGWRADQTWGRVVRLPANFDASLLGIGVTKTLKEWRALGVRRSDGGPLPVKDLT
                     ASVLRPGGEEGRAILVYDNFRALLKWNNSSYFASAVGFLADSME"
     gene            complement(2530..3372)
                     /locus_tag="VGP50_01290"
     CDS             complement(2530..3372)
                     /locus_tag="VGP50_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEV8026028.1"
                     /translation="MRSDESVVPGDNLKGGPSVIILYEHPLSPYAQKCKIALGEKGIA
                     FEAKVPQAFGTGATGDQGFIAQNPRHEVPALVDGDLQIFDSTIILEYLEDKWPEPPLL
                     PKDPAERARLRMLEDVMDTQYEAINWGIMEILGFRRASGALREQLLGRAAEQTAKLQG
                     WLEHKLDGRAWFSGERFGWGDAAVAPHINISVDNGHPPKAGSRLADWLARANARPSVA
                     ACQDAARAILNLKLDFSRVLESGMFRRQYRDHRLEWMMRSGGLPIVLEGLEKKTIRFS
                     NELA"
     gene            complement(3365..4237)
                     /locus_tag="VGP50_01295"
     CDS             complement(3365..4237)
                     /locus_tag="VGP50_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV8026029.1"
                     /translation="MAAAPRERIETYRGIVAPVECDVMGHLTIACYFDRFSDAAFALI
                     DGIAPAAAPPAAAWRSTSLFVRYTKELRAGDGLFIRSGVIGQEGDEIRLGHEVVGSTT
                     GDVMTRIEHTLTPRDLPYGGLGEQRRVLAEGAIPWESPGFEDIAPLNHPERMIDSARD
                     RVKAWEVDERGELALSGLVHRFSAGCMQLCGAIGITPDYIRRERRGFSTFETRLHIEA
                     VPPGAGDAVLLRSGVLGTGNSSVRMLHELFMAKTGESLALFYQSGVHFDLEARRPVPL
                     SPEQVKRAGELVIA"
     gene            complement(4267..4728)
                     /locus_tag="VGP50_01300"
     CDS             complement(4267..4728)
                     /locus_tag="VGP50_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV8026030.1"
                     /translation="MRDAAYRLVYRLGFRVARAWWAIRRQDHYGVMVAVWLEGRVLML
                     RSSYRASFDFPGGGIDPGEAPLAAARRELFEEVGVVAAADAMRFVREIVAWWDNRRDH
                     VSMFELRLAAAPSLRPDGREIVSAAFLPPEAVLALPISPFVRAYLDSVAVA"
BASE COUNT          817 a         1693 c         1723 g          780 t
ORIGIN      
        1 gtcgccgccg aggccggtgc gccaggcatg ctcgctcacc ggcagcatgt cgtcgagctt
       61 cgcccgcccc tccgcgagat aggtataggc cacataggcg gtcatgatct tgctcatcga
      121 cgccgtcgga atgcgctcgt caccgccctt gtcgagcagc actgcgcccg tctggaagtc
      181 gatcgcgaac gcgttgcgcg cgctggtgtc gatgacgggg cggcccgccg gcgccgcggt
      241 caccgccgct gcacggggtg ccggatcctt gttgtccttg cgcgcggcaa gcgccggacc
      301 ggcggtcgcg gccgtcgcca cgagcagact cataatgcaa aagcgcaagg gcgaaagact
      361 cttcatgcat ccactctcga ggttcgaagt gcccgcaagc gagcgagacg gcattctccc
      421 accatgccgg gagattatgc ccaaagtatg aaagcgcatc agtcgacgac gatgcgcgct
      481 tcggtcagtc cggcatcgac gacccggccc agcatctggt cggcctgatc gacgctgccg
      541 atgggcccga ggcgcacgcg gtagagatcg acgcctttga cgcgcgcgcc atagaccttg
      601 accgggccga tcgcgtcgag ccggtccttg acgcggagcg cattcgcggc gaccgcgtaa
      661 gccccgacct ggatgaagat ctgcgtcgcg ccgaccggca ccaccgtgac cttccccgag
      721 aggacgggcg ccggagcggc gggcgcgaga ctcggcgcct cggtcgacgc gatgcgcagc
      781 gacggcggcg ccgccgccag cggctctgtt agcaccggct cgcgcggcgc cgattggggc
      841 acgtcgtcgc tcgccttggc gacgacgtcg ccgccgttgc ggccggcgag cgcggccact
      901 tggagactct ccggcaccag gatcttgacg cggaccttgg ccgtgccctg cgcctcgaat
      961 ccgaggagct gcgcggcgcg gcgcgagacg tcgacgatgc ggccccgcgc ataagggccg
     1021 cggtcgttga cgcgcagcgc catggagcgt ccgttgtcga gattggtgat ctggacgatc
     1081 gagggcatcg gcagcgtgcg atgcgcggcg gtcagcgcgt tcaagtcgaa gatctcgcca
     1141 ttggcggtgt atttgccgtg gaagtcctcg ccataccacg aggcgatgcc ggtctcgtcg
     1201 taggagaagt cttccgccgg tgcgtaccac accccgccga tctgataggg gttgccgatc
     1261 ttgtaagtgc cacggcccgc cgcctcgccg gctctgggcg cggttggcgc cgccggcggc
     1321 gcgtgactgg cgcaggccgc gagaagcgcc gtcgcgagca gcagaaagga ggcggaacgg
     1381 cgtgtcatca cagatctggt gtgtgtcctc gtgatcggcc caacatctcg cgcactctac
     1441 cgtcactcca tgctatcggc aaggaagccc acggcgctcg cgaaatagct ggagttgttc
     1501 catttgagga gtgcgcggaa attgtcgtaa acgaggatcg cccgcccctc ctcgccgccc
     1561 ggccgcagta ccgaggcggt gagatccttg accggaagcg gcccgccgtc gctgcgccgg
     1621 acgcccagcg cccgccactc cttgagcgtc ttggtgacgc cgattccgag caggctcgca
     1681 tcgaaattcg ccggcagtcg caccacccgt ccccaggtct ggtcggcgcg ccagccggat
     1741 tgcgcgaggt aattggccat cgaagcgaag acgtcctcgc gccggtgcca gatgtcgcgc
     1801 ttgccgtcgc cgctatagct caccgcatag gtaaggaagg tcgagggcat gaactgcgcc
     1861 tgccccatgg cgccggccca ggatccctgc agctccgcgg gatcgatgcc gtcctggtcg
     1921 acgattcgca gcgcattcac gagctcggcg cggaagaaag gcccgcgcct gccctcatag
     1981 gcgagcgtcg ccagagcgcc gatgacgggg aagcgcccgg gatcggcgcc atagctcgtc
     2041 tcgattcccc agagcgccac gataaagcgc ggctgcacgc catagcgctg gccgaccgcg
     2101 tcgagcagcg cgcggttgcg ggcgagctgc tcgcgcgccg ccctctgacg atcggcgctc
     2161 acgacatgct cgagatagtc ctcataggtc cggacctttt ccggctggcg gcggtcgagc
     2221 tcgatgacgc gcgggatggg ctcgatcccg tcgagggcgc gggccaccgt cgccggctga
     2281 atgcccgccg cgagcgcttc ctgcttcagg gcttcgagcc aggcggcgaa atccgcgtcc
     2341 tcggcggaag cgggcccggc ggccaggaga agcaccacgg ccaggacgat gcggcgggcg
     2401 agcacgacgg gggatctctt ggatggatgc gcaggcgcaa cgattcgcgg cgcccttggt
     2461 ggcgaggatc gcgccttcat ggttaacaat cggtcaagat gccgtgccat cccgacgaaa
     2521 aggcgaggct caggcaagct cgttgctgaa gcgtatcgtt tttttctcga ggccttcgag
     2581 gacgatcggc aggccgccgc tgcgcatcat ccactcgagg cggtgatccc ggtactgtcg
     2641 gcggaacatg ccgctctcga gcacgcggct gaaatccagc ttgagattga ggattgcgcg
     2701 ggcggcgtcc tggcaagcgg cgacgctggg ccgcgcattg gcgcgcgcga gccagtcggc
     2761 gaggcggctt cccgctttgg gcggatggcc gttgtcgacg gagatgttga tatgcggcgc
     2821 cacggcagcg tcgccccagc cgaaacgctc gccgctgaac cacgcgcgcc cgtcaagctt
     2881 gtgctcgagc cagccttgga gcttcgccgt ctgctcggcc gcgcggccca gcaattgctc
     2941 gcgcagcgcg ccgctcgcgc ggcggaatcc gaggatctcc atgatgcccc agttgatggc
     3001 ctcgtattgt gtgtccatga cgtcctcgag catgcgcagc cgcgcgcgct ccgccggatc
     3061 cttcggcagc agcggcggct ccggccattt atcctcgaga tattcgagga tgattgtgga
     3121 atcgaagatc tgcagatcgc cgtccaccag cgccggcacc tcatggcgcg gattctgcgc
     3181 gatgaagccc tgatcgccgg tggccccggt gccgaacgcc tgcggcacct tggcctcgaa
     3241 cgcgatgccc ttctccccca gcgcgatctt gcatttctgc gcataaggcg agagcgggtg
     3301 ctcgtagaga atgatcacgg agggacctcc cttgagattg tcgccgggca cgacgctttc
     3361 gtcgctacgc aatcaccagc tcgccggcgc gcttgacctg ctccggcgag agcggcaccg
     3421 ggcgtcgcgc ctcgaggtcg aaatgcacgc ccgattgata gaagagcgcg aggctctcgc
     3481 cggtcttcgc catgaagagc tcgtgcagca tgcgcaccga agaattgccg gtgccgagaa
     3541 cgccgctgcg cagcagcaca gcatcgcccg cgccgggcgg caccgcttcg atatggagcc
     3601 gcgtctcgaa ggtggagaag ccgcggcgct cgcggcgaat gtagtcgggc gtgatgccga
     3661 tcgcgccgca gagctgcatg cagccggcgg agaagcgatg gacgaggccc gagagcgcga
     3721 gctcgccgcg ctcgtcgacc tcccacgcct tcacgcggtc gcgtgcgctg tcgatcatgc
     3781 gttcgggatg gttgagcggc gcgatgtcct cgaagcccgg cgattcccag ggaatggccc
     3841 cttcggcgag cacgcggcgc tgctcgccca gcccgccata aggaagatcg cgcggcgtca
     3901 gcgtgtgctc gatccgcgtc atgacgtcgc cggtcgtgct gccgacgacc tcatggccca
     3961 ggcgtatctc gtcgccctcc tggccgatga cgccgcttct gatgaagagc ccgtcgccgg
     4021 cgcgcagctc cttcgtgtag cgcacgaaga ggctggtgct gcgccatgcc gcggcgggcg
     4081 gcgccgccgc gggcgcgatg ccgtcgatca aggcaaaagc ggcatcgctg aagcggtcga
     4141 aatagcaggc gatcgtcaga tggcccatga cgtcgcattc gacgggcgcg acgatgccgc
     4201 gataggtttc gatgcgttcg cgtggtgctg ccgccatgct ggctgcctcc ttgctcgggg
     4261 tcgggactat gccacggcca cgctgtcgag gtaagcgcgc acgaagggcg agatcggcag
     4321 cgccagcacg gcttcgggcg gcaggaaagc ggccgagacg atctcgcggc cgtcggggcg
     4381 gaggctcggc gccgcggcga ggcgcagctc gaacatcgaa acatggtcgc gccggttgtc
     4441 ccaccacgcc acgatctcgc gcacgaagcg catggcatcg gcggctgcga cgacgcccac
     4501 ctcctcgaag agctcgcggc gagcggcggc gagcggcgcc tcgccggggt cgatgccgcc
     4561 gccggggaaa tcgaagctcg cgcggtagga gctgcgcagc atcagcacgc gcccttcgag
     4621 ccacaccgcc accatgacgc cataatggtc ctgccggcgg atcgcccacc acgcccgcgc
     4681 cacgcggaag cccaggcggt agaccaagcg ataagcagcg tcccgcatgc cgtttctatg
     4741 gcacaagaac gacgtcggtg catgcccggc gccgcagcct cttcaagggc tcatttctcc
     4801 tcccccgagg ggagggacgc atcgcgaaaa caatgggagg gtgagcaagg ggatctaagc
     4861 gtagacccgg gtcagctgcg atccgcgacc gccaccaacg acgcggcatt cgacgatgcc
     4921 ggcactgcgt accaggcgag cccgcccagc gctgcggcga tgagacaggc gacgatgacc
     4981 agcttggggt tgccgcggcg ctctgcccag gcc
//
LOCUS       DASYNS010000041         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3599287, whole genome shotgun sequence.
ACCESSION   DASYNS010000041 DASYNS010000000
VERSION     DASYNS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3599287"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VGP50_01305"
     CDS             <1..752
                     /locus_tag="VGP50_01305"
                     /inference="COORDINATES: protein motif:HMM:NF025912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8026031.1"
                     /translation="PMLERAVKEAKGAVRLVKIDIDKNQQLAQQMRIQSIPAVYAFKD
                     GRPVDGFLGAVPESDIKQFVARLGGAKGPSPIEEALALAKEAITEGDMNRAATIFTQV
                     LQREPDNVEALAGLARTAIAKKDFAKARQLLSRVPAEAAGHAEVVAARTGLELAEAGA
                     KAQGALGELRARLERDPNDHQARLDLAGALFAGGNREQAVDELLELVRRDRKWNEEAA
                     RKQLVKYFEAMGGADPLTQSARRRLSSLLFA"
     gene            749..1417
                     /locus_tag="VGP50_01310"
     CDS             749..1417
                     /locus_tag="VGP50_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEV8026032.1"
                     /translation="MSPFVPRFAALPSALPIFPLTGALLLPRGELPLNIFEPRYLAMT
                     RDALAGERLVGMVQPLGDKGDAGEPAVYRIGCAGRLTQFSETEDGRYLITLTGLLRFD
                     IVEELPREGRLYRRVVPDWGRYRGDLDEQHEQHAPLARERFLAALKPFFARHQIAADW
                     QALATADDERLVTAIAMVGPFAPSEKQALLEATDTAERARLLTALLEMAARGGDAGDT
                     SARH"
     gene            1437..1661
                     /locus_tag="VGP50_01315"
     CDS             1437..1661
                     /locus_tag="VGP50_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEV8026033.1"
                     /translation="MADPATDVDPRLLEILVCPLTKAPLIYDRAAQELISVEAGLAYP
                     IRDGIPIMLVDEARPLDEVEKTKHRAKPAP"
     gene            1658..2026
                     /locus_tag="VGP50_01320"
     CDS             1658..2026
                     /locus_tag="VGP50_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEV8026034.1"
                     /translation="MSAASERWPVEIRLKRAEKILEISFDDGSRFRLPAEYLRVESPS
                     AEVQGHGPGQKQLVAGRAQVGITAVEPVGNYAVRLRFDDGHDTGIYSWRYLHELGDQQ
                     ERRWRDYLGALQKAGLSREK"
BASE COUNT          353 a          745 c          700 g          289 t
ORIGIN      
        1 ggcccatgct cgagcgcgcg gtcaaggaag ccaagggcgc ggtgcgcctc gtcaagatcg
       61 acatcgacaa gaaccagcag ctggcgcagc agatgcgcat ccagtcgatc cccgcggtct
      121 atgccttcaa ggacggccgc ccggtcgatg gcttcctcgg cgccgtgccg gagagcgaca
      181 taaagcagtt cgtcgcgcgg ctcggcggcg ccaagggccc gtcgccgatc gaggaagcgc
      241 tggcgctcgc caaggaggcg atcaccgaag gcgacatgaa ccgcgccgcc accatcttca
      301 cccaggtgct gcagcgcgag cccgacaacg tcgaggcgct ggccggcctc gcgcgcaccg
      361 ccatcgccaa gaaggacttc gccaaggcgc gccagctcct ctctcgcgtg cccgccgaag
      421 ccgcgggcca tgccgaggtc gtggcggcgc gcaccgggct cgagctcgcc gaagccggtg
      481 ccaaggcgca aggggcgttg ggcgagctca gggcgcggct cgagcgcgat cccaacgacc
      541 atcaggcgcg gctcgatctc gcgggcgcgc tcttcgccgg cggcaatcgg gagcaagcgg
      601 tcgacgagct cctcgagctg gtccggcgcg accgcaaatg gaatgaagaa gcggcgcgga
      661 agcagctcgt caaatacttc gaggcgatgg gcggggccga tcctttgacg cagtcggcgc
      721 gccgccgcct ttcgtcgctc ctcttcgcat gagccctttc gtcccgcgct tcgcggcgct
      781 cccgtcggcg ctgccgatct ttccgctgac gggcgcgctc ctcctgccgc gcggcgagct
      841 gccgctcaac atcttcgagc cgcgctacct cgccatgacg cgggacgcgc tcgcgggcga
      901 gcgccttgtc ggcatggtgc agccgcttgg agacaagggc gatgccggcg agcccgcggt
      961 ctaccgcatc ggctgcgccg ggcggctcac ccagttcagc gagacggagg acggccgcta
     1021 tctcatcacg ctgaccgggc ttctgcgctt cgacatcgtc gaagagctgc cgcgcgaggg
     1081 ccggctctac cgccgcgtcg tgccggattg ggggcgctat cgcggcgacc tcgacgagca
     1141 gcatgagcag cacgcgccgc tcgcgcgcga gcgtttcctc gccgcgctca agcctttctt
     1201 cgcgcgccac cagatcgccg ccgactggca ggcgctcgcg acggccgacg acgagcgcct
     1261 cgtcaccgcc atcgccatgg tcggtccctt cgcgcccagc gagaagcagg cgctgctcga
     1321 agcgaccgac acggccgagc gtgcacgact cctgaccgcg ctcctcgaga tggcggccag
     1381 gggcggcgac gccggcgaca caagcgcgcg gcattgacga cggagacaca tgcgtcatgg
     1441 ccgatccagc gaccgacgtc gatccacggc tcctcgagat cctggtttgc cccttgacca
     1501 aggcgccact catctatgac cgcgcggcgc aggagttgat cagcgtcgaa gcgggactcg
     1561 cctatccgat tcgcgacggc atccccatca tgctggtcga cgaggcgcgt ccgctcgacg
     1621 aggtcgagaa gacgaagcac cgggcgaagc ccgcgccgtg agcgcggcgt ccgagcgctg
     1681 gcccgtcgag atccgcctca agcgcgcgga aaagatcctg gagatctcgt tcgacgacgg
     1741 cagccgcttt cgcctgccgg ccgaatatct gcgcgtcgaa agcccctctg ctgaagtcca
     1801 gggccacggc cccggccaga agcagctcgt cgccggccgc gcgcaagtcg gcattaccgc
     1861 cgtcgagccc gtcggcaact atgccgtccg gctgcgcttc gacgacggcc acgacaccgg
     1921 catctactcc tggcgctatc tccacgaact cggcgaccag caggagcggc gctggcgcga
     1981 ttatctcggc gcgctgcaaa aggccgggct atcccgcgag aaataaaaag aggaacgcgg
     2041 aggacgcgga ggtttacgca aaggacgcgg aggaacaaaa tcctctg
//
LOCUS       DASYNS010000042         2389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3131920, whole genome shotgun sequence.
ACCESSION   DASYNS010000042 DASYNS010000000
VERSION     DASYNS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3131920"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VGP50_01325"
     CDS             complement(<1..308)
                     /locus_tag="VGP50_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424363.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HEV8026035.1"
                     /translation="MTACIVGWAHSRFGKLDGEDLESLLARAASEAIRDAGIAAADID
                     EIFIGTFNGGFARQEFPSSLALQVDPALRFKPATRVENACATGSAAIHQGLNAIAARR
                     A"
     gene            536..1576
                     /gene="pyrC"
                     /locus_tag="VGP50_01330"
     CDS             536..1576
                     /gene="pyrC"
                     /locus_tag="VGP50_01330"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV8026036.1"
                     /translation="MSPVSVTLRRPDDWHVHLRDGAMMAAVLPFTARSFARAIVMPNL
                     APPVTTAALAEAYRTRILKALPKGLAFTPLMTAYLTDESDADDIERGHRAGIFTAVKL
                     YPAGATTHSAAGVTDIRRTDRVLEKLAKIGMPLLVHGEVTDPAVDIFDREAVFIERVL
                     APLMKRLPELKIVLEHITTKEAVDFVTASGANLAATITAHHLEINRNALFAGGLRPHM
                     YCLPVAKREAHRLALRRAALSGNSKFFLGTDSAPHPVAEKEQDCGCAGIFSAPAALEI
                     YAQIFAEENALYRLEAFASLNGARFYGLPLNEDRITLEARRWTVPKEVASAAGPVRPF
                     RAGEELAWRRAP"
     gene            complement(1583..2026)
                     /locus_tag="VGP50_01335"
     CDS             complement(1583..2026)
                     /locus_tag="VGP50_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026037.1"
                     /translation="MKRRARWAFLLLVPLLLAVAPARADQGGFHQGFQGHPGSFMHQN
                     GRMFFRPGGRVFFRHDARFFNHDRRFFHHRAFFRPFFFFGTGIFAPLPIALFPPPAPP
                     AYLMGQEEGDPSCYDYQMSGQYGGEPPYGAACVEPDGSWQVAPGY"
     gene            complement(2098..>2389)
                     /locus_tag="VGP50_01340"
     CDS             complement(2098..>2389)
                     /locus_tag="VGP50_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEV8026038.1"
                     /translation="LAEHVPAAEPMARKRGFTVPVGDWIARRSAQLGALVARQPGLRE
                     ICLPQAVEALFRDPKGRHGRFAAWTLLFYALWHRQHIERLPAGPDVFATLGG"
BASE COUNT          411 a          830 c          801 g          347 t
ORIGIN      
        1 gcgcggcggg cggcgatggc gttcagaccc tgatggatcg ccgccgagcc cgtggcgcag
       61 gcattctcca cccgcgtcgc cggcttgaag cgcagcgccg gatccacctg cagcgccagc
      121 gacgacggaa actcctggcg ggcgaagccg ccgttgaacg tgccgatgaa gatctcgtcg
      181 atgtcggccg cggcgatgcc ggcgtcgcgg atggcctcgc tcgcggcccg cgccagcagc
      241 gattcgagat cctcgccgtc gagcttgccg aagcgcgaat gcgcccagcc gacaatgcaa
      301 gcggtcatgg ctgctcctcg agaaatccct ctccccttgt gggagaggga ggggcccatt
      361 gcgcagcaat gggagggtga gggggttgct tccgctgctt caccttcagg atcgacatct
      421 cggcaccagc cccctcaccc tcccgtcgct ttcgcgacgg gcccctccct ctcccacaag
      481 gggagagggg attctatgat gcgctccaca agaagccgct acggtcgata tagcaatgag
      541 ccccgtcagt gtcaccctgc gccggcccga cgactggcat gttcatctgc gcgacggcgc
      601 gatgatggcg gcggtgctgc ccttcaccgc ccggagcttc gcccgcgcca tcgtcatgcc
      661 gaacctggcg ccgccggtca ctaccgccgc tttggccgag gcctatcgca cgcgcatcct
      721 gaaggcgctg ccgaagggtc tcgccttcac gccgctgatg accgcctatc tcaccgacga
      781 gagcgacgcc gacgacatcg agcgcggcca tcgcgcaggg atcttcacgg cggtcaagct
      841 ctaccccgcg ggcgccacga cccattcggc cgcgggcgtc acggacatcc ggcgcaccga
      901 tcgcgtgctc gaaaagctgg cgaagatcgg catgccgctg ctcgtccatg gcgaagtgac
      961 cgatcccgcg gtcgacatct tcgaccgcga ggccgtgttc atcgagcgcg tcctcgcgcc
     1021 gctgatgaag cgcctgccgg agctcaagat cgtgctcgag cacatcacca cgaaagaggc
     1081 ggtggacttc gtcacggcgt cgggcgccaa cctcgcggca accatcaccg cacatcacct
     1141 ggagatcaac cgcaacgccc tcttcgccgg cgggttgcgg ccgcacatgt attgcctgcc
     1201 ggtggcaaag cgcgaggcgc atcgcctggc gctgcgccgc gcggcccttt cgggaaattc
     1261 caaattcttc ctcggcaccg acagcgcgcc gcatcccgtc gcggaaaagg agcaggattg
     1321 cggctgcgcc ggcatcttca gcgcgcccgc cgcgctcgag atctatgccc agatcttcgc
     1381 cgaggagaac gcgctctacc gtctcgaagc cttcgcctcg ctcaacggcg cgcgcttcta
     1441 cggcctgccg ctcaacgagg atcgcatcac gctcgaggcg cggcgctgga cggttccgaa
     1501 ggaagtagca agcgctgccg gcccggtgcg gcccttccgc gcgggcgaag agctcgcctg
     1561 gcgccgcgcg ccctgaggcc gctcagtagc cgggggcgac ctgccaggaa ccgtcgggtt
     1621 cgacgcaggc ggccccgtag ggtggctcgc cgccatactg gcctgacatc tgatagtcgt
     1681 agcaggaggg gtcgccttcc tcctgcccca tcagataggc gggcggcgcg ggcggcggga
     1741 atagcgcgat cggcagcggc gcgaagatgc cggtgccgaa gaagaagaag ggccggaaga
     1801 aagcgcgatg gtgaaagaag cgacggtcgt gattgaagaa ccgcgcgtca tggcggaaga
     1861 acacccgccc gcccgggcgg aagaacatgc gcccgttctg gtgcatgaag ctgcccggat
     1921 gtccttgaaa tccctggtgg aaaccgccct gatcggcgcg cgccggcgca accgcgagca
     1981 gcaaggggac gagcagcagg aacgcccaac gggcacggcg tttcatcttg gcctcccaag
     2041 caacctgctc attatacgct tcgaggcgcc ggaacgtgca taacagagtt gtgacgctca
     2101 gccgcccagc gtcgcgaaca catcgggacc ggcgggcagc cgctcgatgt gctggcggtg
     2161 ccagagcgca tagaagagca gcgtccaggc ggcaaagcgc ccgtggcgtc ccttgggatc
     2221 gcggaagagc gcctcgaccg cctggggcag gcagatctcg cgcagcccgg gctggcgggc
     2281 gacgagggcg ccgagctggg ccgagcgccg cgcgatccaa tcgccgaccg ggaccgtgaa
     2341 gccgcgcttg cgcgccatgg gctccgccgc gggcacatgc tcggcgagc
//
LOCUS       DASYNS010000043         6721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2977072, whole genome shotgun sequence.
ACCESSION   DASYNS010000043 DASYNS010000000
VERSION     DASYNS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2977072"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VGP50_01345"
     CDS             complement(<1..185)
                     /locus_tag="VGP50_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026039.1"
                     /translation="MPSPPLDAPSPPAGSARPKRRRWLWPPIGLALAYILANCALYGM
                     QRSLVFQPSTGGEPARA"
     gene            356..1321
                     /gene="hemB"
                     /locus_tag="VGP50_01350"
     CDS             356..1321
                     /gene="hemB"
                     /locus_tag="VGP50_01350"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEV8026040.1"
                     /translation="MRRNRRDEWSRRLVAENVLTAADLIWPVFVHDERAARAPVPSMP
                     GVDRLSIAALVDAAGEALALGIPVIALFPATPAERKTPDGEESVNPENLVCRAVRAVK
                     KALPSLGVLCDVALDPFTTHGHDGLLRDGYVANDETVEVLCRQALVQAEAGCDVIAPS
                     DMMDGRVGAIRDALDAKGFDRVRIMSYAAKYASAFYGPFRDAVGSKSSLGKADKRGYQ
                     MDPANSDEALREVALDLAEGADMVMVKPGLPYLDIIRRVKERFGAPTFAYQVSGEYAM
                     LTGAVERGWLDPSVIIEALLAFKRAGADGVLTYSAVDAARRLKAG"
     gene            complement(1335..1466)
                     /locus_tag="VGP50_01355"
     CDS             complement(1335..1466)
                     /locus_tag="VGP50_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026041.1"
                     /translation="MEASERSGNAGNVVKLVLAWSFVGIPLALGVAQTLSNAMKLFQ"
     gene            complement(1485..2882)
                     /locus_tag="VGP50_01360"
     CDS             complement(1485..2882)
                     /locus_tag="VGP50_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV8026042.1"
                     /translation="MSGLSFLSKERIVARPGYSRWLIPPAALCVHLCIGQAYAFSVFN
                     LPMTKLLGITASTPDDWTIPELGWIFSIAIAVLGLSAALLGRWVEEGGPRKAMFTAAL
                     CWGGGFVVSSIGVYIHQIWLIYLGYGVLGGFGLGIGYISPVSTLIKWFPDRPGMATGM
                     AIMGFGGAAFIASPLSVWLMAEFSTPEHVGVAETFLLMGAIYVCFMMVGAVIVRVPAP
                     GWKPAGYVAPVQPKKLITTQNVYVYDAVKTPQFWLIWLVLCLNVTAGIGVLGQASAMS
                     QEMFPGRVTPVAAAGFVGLMSLFNMGGRFLWASVSDYIGRKNTYFIFFLLGALLYAAV
                     PYTGEIGALPLFVAAYAVIYTMYGGGFATVPAYLKDMFGTRYVGAIHGLLLTAWSVAG
                     VAGPALVNYIREYNLAHGVAKAQAYNVTMYVMAGLLVVGFICNYFVTAVHERYHMKRR
                     EAGAETETETGAATA"
     gene            3372..3737
                     /locus_tag="VGP50_01365"
     CDS             3372..3737
                     /locus_tag="VGP50_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8026043.1"
                     /translation="MNSPFTILLVEDDSAVRDVVVQILLSKGFQVLVATDGYEAIRLL
                     VAQHVDVMLTDIMMPGLSGYELAAQARLIRPALRILYTTGYDGQAAGRDMAGGYGKLL
                     PKPVRADELVSEILHLLKA"
     gene            complement(3747..4760)
                     /locus_tag="VGP50_01370"
     CDS             complement(3747..4760)
                     /locus_tag="VGP50_01370"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV8026044.1"
                     /translation="MDSLRLNASPAIAAADFPPFLMRAPWWGGDLQTLRNFLMRRRPA
                     LAAPERLLLPMRDGSGDRLLAAVHRPVPAAVKPRPLAFLLHGLSGCEDSAYVLNTASH
                     LLALGFPVLRLNLRGAGPSRPFCRFQYHAGRSEDLADALAALPADLVTAGVVAIGYSL
                     GANMLLKFLGERGGEAPVKAAVAVSAPLDLAVAARQMMRRRNVLYQGYLLRHMRAESL
                     APGAEVTAAERAAIREARSIWEFDHGFSAPRNGFGGAEDYYERNAARRYLDGIAVPTL
                     VIHALDDPWIPGAPYLAYDWRRNPSLVALLPPRGGHVGFQGRHDRRIPWHDLCAAQFL
                     AAF"
     gene            complement(4742..5665)
                     /locus_tag="VGP50_01375"
     CDS             complement(4742..5665)
                     /locus_tag="VGP50_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV8026045.1"
                     /translation="MPSSSAPESGVTGYLAPEGYLPELLAELGDAVTETHGRLVLAKG
                     ASRPAAWVANLWRDPRRLPIASIGDAVRQLRAIQRNWVLYSHAHHRRSALIAEQLPKV
                     SAKPLVFGMPAPKAPLGSWTLLAADTLLASPDCTSPFPNGEVSFVENHDMPPSRAYLK
                     LWELFTLLGEHPRPGERCLDLGGSPGGWTWVLQQLGADVVSVDKAPLDPRIAALPRIE
                     VRKESAFALDPASVGGIDWLFSDVVCYPERLLRLVERWLAAADCQRFVCTIKFQGSTD
                     HEIARRFASIPGSRLLHLHHNKHELTWIRFA"
     gene            complement(5643..6488)
                     /locus_tag="VGP50_01380"
     CDS             complement(5643..6488)
                     /locus_tag="VGP50_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8026046.1"
                     /translation="MIARSLDCLGPHGFHRVAYWEWSGPEKGRTVICAHGLTRNGRDF
                     DVLAADLASEHRVICPDIVGRGKSDWLVEPADYSYDVYLADLAALIARLDVGEVDWVG
                     TSMGGLLGMMMAAKPRSPIRRLVINDIGAVVAKEGLARIGSYVGQDPSFRDLHELEAE
                     LRRLFPSLGSLSDLQWRELAMQGARQKPDGSLGFAYDPRIGDPFRTEPEADIDLWSVY
                     DAVRCPTLLLRGAESDVLRRADAVAMTERGPRAQLVEFAGVGHAPSLATPDQIAPVRA
                     FLLGP"
BASE COUNT         1208 a         2238 c         2223 g         1052 t
ORIGIN      
        1 tccgcgcggg caggctcgcc gccggtcgag ggctggaaga ccagcgagcg ctgcatgccg
       61 tagagggcgc aattggcgag gatgtaggcg agggcgagcc cgatcggcgg ccagagccag
      121 cgccggcgct tcggccgcgc agacccggcg ggagggctcg gtgcatcgag tggtggcgag
      181 ggcatggcgg aagcttacgt ccaaaaccag ccgcaatcat cgttaacgcg cgggcgccgc
      241 gatttgccag cgccatggac cccgcttatc atgtttttcg tgtgttgagc cggacgagga
      301 tcgacccgtg acccgttccc cttcgcagcc gcttggcgcc tatccgcgca tccgcatgcg
      361 ccgcaaccgt cgcgacgagt ggtcgcggcg cctcgtggcc gagaacgtcc tcaccgccgc
      421 cgatctcatc tggccggtct tcgttcatga cgagcgcgcc gcgcgggcgc cggtgccgtc
      481 gatgcccggc gtcgaccgcc tgtcgatcgc ggcgctggtc gatgcggcgg gcgaagcgct
      541 ggcgttgggc atccccgtca tcgccctgtt cccagcgacg ccggccgagc gcaagacgcc
      601 cgacggggag gagtccgtca atcccgagaa cctcgtctgc cgtgccgtgc gcgcggtgaa
      661 gaaggccctg ccgtcgctcg gcgtgctgtg cgacgtggcg ctcgacccct tcacgacgca
      721 tggtcatgac gggctgctgc gcgacggcta cgtggcgaat gacgagacgg tcgaggtgct
      781 gtgccgtcag gcgctggtgc aggccgaggc cggctgcgac gtcatcgccc cttccgacat
      841 gatggatggc cgcgtcggcg cgatccgcga cgcgctcgac gccaaggggt tcgaccgcgt
      901 gcgcatcatg tcctacgcgg ccaaatacgc ctcggccttc tacgggccct tccgcgacgc
      961 cgtgggctcc aaatcctcgc tcggcaaggc cgacaagcgc ggctatcaga tggaccccgc
     1021 caacagcgac gaggcgctgc gcgaggtggc gctcgacctc gccgagggcg ccgacatggt
     1081 gatggtgaag cccggcctcc cctatctcga catcatccgc cgcgtcaagg agcgcttcgg
     1141 cgcgccgacc ttcgcctatc aggtgagcgg cgaatacgcc atgctgacgg gcgcggtcga
     1201 gcgcggctgg ctcgatccga gcgtgatcat cgaggcgctc ctcgccttca agcgcgccgg
     1261 cgccgacggc gtgctgacct attcggcggt cgacgccgcc aggcgattga aggcggggta
     1321 ggacacgccc gtcgctactg gaagagcttc atggcgttgc tcagggtctg agcgacaccc
     1381 aaggcgagcg gaatgccgac gaagctccaa gccaggacga gcttcaccac gttgccggcg
     1441 ttacccgatc tttcgcttgc ttccatggtg cttctccgat ccctctacgc cgttgccgcc
     1501 cctgtctcgg tctcggtctc tgcccccgct tcgcgtcgct tcatatggta acgctcgtga
     1561 acggcggtga cgaagtagtt gcaaatgaaa ccgacaacca gcagccccgc catcacgtac
     1621 atggtgacgt tgtaggcctg cgccttggcg actccgtgcg cgagattata ttcgcgaatg
     1681 tagttgacga gagccggccc agcgacgcca gccaccgacc acgccgtcag cagcaaaccg
     1741 tggatggcgc caacgtagcg cgtgccgaac atatccttga gataggcggg caccgtggcg
     1801 aagccgccgc catacatcgt gtaaatcacc gcgtaggcgg cgacaaacag cggcagagct
     1861 ccaatctctc cggtgtaggg tacggccgca tagagcagcg cgccgagcag gaagaagatg
     1921 aaataggtat tcttccgtcc gatgtagtcg gacaccgacg cccataagaa gcgaccgccc
     1981 atgttgaaga gactcatcaa gccgacaaag ccggctgccg caaccggtgt cacgcgtccc
     2041 gggaacatct cctggctcat cgccgaggcc tggccgagca caccgatgcc ggcggtcacg
     2101 ttgagacaca gcacgagcca gatgagccag aattggggcg tcttgacggc gtcatagaca
     2161 tagacattct gggtcgtgat gagtttcttg ggttgcactg gcgccacata gcccgccggc
     2221 ttccaaccgg gcgccggcac gcgcacgatg accgcgccaa ccatcatgaa acagacatag
     2281 atcgcgccca tgagcaggaa ggtttcggcc acaccgacat gctcgggcgt cgagaattct
     2341 gccatgagcc agacggagag cggggaagcg atgaaggcgg cgccgccgaa gcccatgatc
     2401 gccattcccg tcgccatgcc gggccggtcc ggaaaccatt tgatcagcgt cgagaccggg
     2461 gagatatacc cgatgccaag gccgaagccg ccgagcacgc catagccaag gtagatcagc
     2521 cagatctggt gaatgtagac gccgatcgac gagacgacga agccgccgcc ccagcagagc
     2581 gccgccgtga acatcgcctt gcgcggcccg ccctcctcta cccagcgtcc gagcaaggcc
     2641 gccgacagtc caaggacggc gatggcgatg ctgaagatcc agccgagctc cggtatcgtc
     2701 caatcgtcgg gcgtcgatgc ggtaatcccg aggagcttgg tcatgggcag gttgaagacg
     2761 ctgaacgcgt aagcctggcc gatgcacaga tgaacgcaga gcgccgcggg cgggatcagc
     2821 catcggctgt atccggggcg agcgacaatg cgctccttgc tcaaaaaaga aaggcccgac
     2881 atcgcgactt ctccctttat cgcagctcgc gttatggttg gttgtagtgc cacaatcgct
     2941 ttgcacatac ataagcaact ttgcccctga atccgcaaca aaaaagggcg gtcggcgacg
     3001 gggtcgtggc gctccggacg ggaacttcgc agcaaaatgc gcgttgctga accacatcat
     3061 gcgtcgcctc attacttcct cgtggttgaa tatccgctcg catcgccagc taatcacgac
     3121 ggcgctcgca gtaaaacacg acgcagccca ccctcctaaa gagactctcc gtcgcacttt
     3181 agtgtcgatc gtaaagacgg cccacatcag tcccgctggc gacgtctttc tctctattct
     3241 tggttgcccg ctttatgtcc ctgctttgtt acaatctcgg gctcgcggcg agaagttgaa
     3301 attgctattt tccgtcccgc atctgggcgt ttctggagcc cgcaggcgta acgcgtgttt
     3361 atagcgacgg catgaactct ccgtttacca tcttgctcgt cgaagacgat agcgccgtgc
     3421 gcgatgtcgt cgtgcagatc ctgctgtcca agggctttca ggtcctggtc gccaccgatg
     3481 gctacgaggc gatccgtctg ctcgtcgcgc agcatgtgga cgtgatgctc acggacatca
     3541 tgatgccggg cttgagcggc tacgagctcg cggcccaggc gcgtctgatc cggcccgcgc
     3601 tgcgcatcct ctacaccacc ggctatgacg gccaggccgc cggccgcgac atggccggag
     3661 gctatggcaa gcttttgccc aagcccgttc gcgccgacga gctggtcagc gagatcctcc
     3721 acctcctcaa ggcttgagcg cccgcttcag aaggccgcca ggaactgggc cgcacagagg
     3781 tcatgccagg gaatgcggcg atcatggcgg ccctggaagc cgacatggcc gccccgcggc
     3841 ggcagcagcg ccaccaggct cggattgcgc cgccagtcat aggcgagata gggcgccccc
     3901 gggatccacg gatcgtcgag cgcatggatc accagggtcg gcacggcaat gccgtcgaga
     3961 tagcgccgcg cggcattgcg ctcgtaatag tcctcggcgc cgccgaagcc gttgcgcggc
     4021 gcgctgaagc cgtggtcgaa ctcccagatg ctgcgcgcct cgcggatcgc cgcgcgctcg
     4081 gcggcggtga cctccgcgcc gggtgccagg ctctccgccc gcatatgccg gagcagatag
     4141 ccctgataga ggacgttgcg ccgccgcatc atctggcgcg cggcgacggc gagatcgagc
     4201 ggcgcggaca ccgccaccgc cgccttgacc ggcgcctccc cgccccgctc gccgaggaac
     4261 ttcagcagca tgttggcgcc gagcgagtag ccgatcgcga ccacgccggc cgtcacgaga
     4321 tccgccggca gcgcggccag cgcgtcggcc aggtcctcgc tccggcccgc gtggtattgg
     4381 aagcggcaga aggggcgcga cggcccggcg ccgcgcaggt tgaggcggag cacgggaaag
     4441 ccgagcgcca gcagatggga cgcggtgttg aggacgtagg cgctgtcttc gcagccgctg
     4501 agcccgtgca agaggaaggc gagcggccgc ggcttcaccg ccgccggcac cggccgatgg
     4561 acggccgcga gcagccggtc gccgctgccg tcacgcatgg gcaggaggag ccgctccggc
     4621 gccgcgagcg cgggccgtcg ccgcatcagg aaattcctga gcgtctgcag atcgccgccc
     4681 caccacggcg cgcgcatgag gaagggcggg aaatccgcgg cggcgatcgc cgggctggcg
     4741 ttcaggcgaa gcgaatccat gtcagctcat gcttgttgtg atggagatga agaagccggg
     4801 agccgggaat ggaagcgaaa cgccgcgcaa tctcatggtc ggtcgagcct tgaaacttta
     4861 tcgtgcacac gaaacgctgg cagtccgccg cggcgagcca acgctcgacg aggcgaagca
     4921 accgctccgg ataacaaaca acgtccgaga acaaccaatc tatccctccg acactcgctg
     4981 gatcgagagc gaaggcgctt tccttccgga cttctatccg gggtagtgcg gcgatccgcg
     5041 ggtcgagcgg cgccttgtcg acgctcacca cgtcggcgcc caattgctgc agcacccagg
     5101 tccatccgcc cgggctgccg ccgaggtcga ggcagcgctc gcctggccgg gggtgctccc
     5161 cgaggagcgt gaacagctcc cagagcttca aataagcgcg gctcggcggc atgtcgtggt
     5221 tctcgacgaa gctcacctcc ccgttgggga aggggctggt gcagtcgggt gacgccagca
     5281 gcgtgtcggc ggcgagcagc gtccaggacc ccagcggcgc ctttggcgcc ggcatgccga
     5341 agaccaacgg cttcgccgag accttcggca gctgctcggc gatgagcgcg gagcggcggt
     5401 gatgggcatg ggaataaagc acccagttgc gctggatcgc gcgcagctgg cgcacggcat
     5461 cgccgatcga ggcgatgggg agacggcgcg ggtcgcgcca cagattggcg acccaggccg
     5521 cgggacgtga cgctcccttc gccagcacga ggcggccatg ggtctcggtg acggcatcgc
     5581 ccagctccgc caggagctcc gggagatagc cctcgggtgc cagatagccg gtgacgccgc
     5641 tctcaggggc cgaggaggaa ggcacggacg ggagcgatct ggtcgggcgt cgccagcgac
     5701 ggcgcatgcc cgacgccggc gaattcgacg agctgcgcgc gcgggccgcg ctcggtcatc
     5761 gccaccgcat ccgcgcgccg cagcacatcg gattccgcgc cgcgcagcag cagcgtcgga
     5821 cagcgcacgg cgtcgtagac cgaccagaga tcaatatccg cttccggctc ggtgcggaag
     5881 ggatcgccga tccgcggatc ataagcgaag cccaggctgc catcgggctt ctgccgcgca
     5941 ccctgcatcg cgagctcacg ccattgcagg tcgctcagcg agcccaagga cgggaagagg
     6001 cggcgcaact cggcctcgag ttcgtggaga tcgcggaagc tcggatcctg gccgacatag
     6061 gagccgatgc gggcgaggcc ctccttggcg acgacggcac cgatatcgtt gatgacgagg
     6121 cggcggatcg gcgagcgtgg ctttgccgcc atcatcatgc cgagaaggcc gcccatggag
     6181 gtgcccaccc aatccacttc gccgacgtcg aggcgcgcga tcagcgccgc caggtccgcg
     6241 agatagacgt cgtagctgta atccgccggc tccaccagcc aatcgctctt gccgcgtccg
     6301 acgatgtcgg gacagatcac gcggtgttcg ctcgcgagat cggctgccag gacgtcgaag
     6361 tcgcggccat tgcgcgtgag cccgtgggcg cagatgacgg tgcgcccctt ttcaggtccc
     6421 gaccattccc aataggcgac gcggtgaaac ccatgcgggc ccaggcaatc gaggctccgc
     6481 gcgatcatgg acataggcgg ttttccctgt taggcgtccc gaccatcact cagcgccgcc
     6541 aagggatcaa gcccgacatg gacgatatcg ccattgcgcc gccgcgcgcc gaggaccagg
     6601 ccgattggcg ccgcctctac gacggctacg ccgccttcta caaatcaccg atggatgacg
     6661 cgatcgccgg acgggtgtgg ggctggctcc tcgacccgca gcacccgctc gaagcgctcg
     6721 t
//
LOCUS       DASYNS010000044         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4692532, whole genome shotgun sequence.
ACCESSION   DASYNS010000044 DASYNS010000000
VERSION     DASYNS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4692532"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VGP50_01385"
     CDS             complement(<1..1190)
                     /locus_tag="VGP50_01385"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEV8026047.1"
                     /translation="MATEVLMPALSPTMTEGKIAKWLKKPGDAVKAGDALAEIETDKA
                     TMEIEAVDEGTLARILVPEGTEHVAVNLPIAVIAGEGESVDPAAKAAPPAPAAPVEPV
                     AETRPPRPAAAASRLPQAPPEQAAPEEKAWTGATVTLTVREALRDAMAEEMRRDDAVF
                     LMGEEVAEYQGAYKVSQGLLQEFGARRVIDTPISEHAIAGIGVGAAFGGLRPIVEFMT
                     WNFAMQAMDQLVNSAAKTRYMSGGQMSCPIVFRGPNGAAARVAAQHSQDYASWYAHVP
                     GLKVIAPYTAADHKGLLKSAIRDPNPVIFLENELVYGATFPVPADGDFTVPIGRARVA
                     RAGDGVTILAYSRMVQVALAAAETLAAAGISAEVIDLRTLRPLDIAAIVASVKKTNRV
                     VSVE"
     gene            complement(1330..>2022)
                     /locus_tag="VGP50_01390"
     CDS             complement(1330..>2022)
                     /locus_tag="VGP50_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV8026048.1"
                     /translation="SKGKGGSMHMFSPEKNFFGGHGIVGAQVPIGTGLGFAHKYREDG
                     KVSITFLGDGAMNQGQVYESFNMAALWKLPVVYIIENNRYGMGTAITRSSASHDLVSR
                     GAPFEIPGHQVDGMNVVAVKQAAEEALDYARSGKGPYIVEMQTYRYRGHSMSDPAKYR
                     SKDEVSRMRQEHDPIDQVKKRLIDSGMADENLLKEIDKEIKDIVTAAADFAQQSPEPD
                     PAELWTDVYADA"
BASE COUNT          292 a          699 c          663 g          368 t
ORIGIN      
        1 tcctccaccg agacgacgcg gttggtcttc ttcaccgagg cgacgatagc ggcgatgtcg
       61 agcgggcgga gcgtgcgcaa atcgatcact tcggccgaga tgcccgcggc ggcgagcgtc
      121 tcggccgccg cgagcgccac ctgaaccatg cgcgaataag cgaggatggt gacgccgtcg
      181 cccgcgcgcg cgacgcgggc gcggccgatc ggcacggtga agtcgccatc ggccggcacg
      241 gggaaggtcg cgccatagac cagctcgttt tcgaggaaga tcactggatt gggatcgcgg
      301 atcgccgatt tgagcaggcc cttgtgatcg gccgcggtat agggcgcgat cactttgagg
      361 cccggcacat gggcatacca gctcgcatag tcctgcgagt gctgcgccgc gacgcgcgcg
      421 gctgcgccgt tggggccgcg gaagacgatg ggacagctca tctgcccgcc cgacatgtag
      481 cgcgtcttgg ccgccgaatt gaccagctgg tccatcgcct gcatggcgaa gttccaggtc
      541 atgaactcga cgatgggacg caagccgccg aacgcggcgc cgacgccgat gccggcgatc
      601 gcatgctcgg agatcggggt gtcgatgacg cggcgcgcgc cgaattcctg cagcaggccc
      661 tggctcacct tgtaggcgcc ctggtattcg gcgacctcct cgcccatgag gaagacggcg
      721 tcgtcgcggc gcatctcctc ggccatggca tcgcgcagcg cctcgcgcac ggtgagcgtc
      781 accgtcgcgc cggtccacgc cttctcctcc ggcgccgcct gctccggtgg cgcctggggc
      841 agccgcgacg ccgctgcggc cggccgcggc ggcctggtct cggcgaccgg ctcgacgggc
      901 gccgcgggcg cgggcggcgc cgctttcgcc gccggatcga cgctctcgcc ttcgccggcg
      961 atcaccgcga tcggcaggtt gaccgcgaca tgctcggtgc cttcgggcac gaggatccgg
     1021 gcgagcgtcc cttcgtcgac cgcttcgatc tccatcgtcg ccttgtcggt ctcgatctcg
     1081 gccagggcat cgcccgcctt caccgcatcg cccggcttct tcagccactt cgcgatcttg
     1141 ccctcggtca tcgtcggcga cagcgccggc atcagcactt cagtggccat gccgttcccc
     1201 cggatggaag aggaacgcag aggacgccgt ggtttcgcgg aggacgcgga ggctatttga
     1261 cgaaatcacc tctgcgtcct ccgcgaaacc tctgcgccct ctgcgttcct cttttccatc
     1321 cgccgcccct caagcatccg cgtacacgtc ggtccagagc tccgccggat cgggctccgg
     1381 gctttgctgc gcgaaatccg cggcggccgt gacgatgtcc ttgatctcct tgtcgatctc
     1441 cttgagcaaa ttctcgtccg ccatgccgct gtcgatcagg cgcttcttca cctggtcgat
     1501 cggatcatgc tcctggcgca tgcggctcac ttcgtccttg ctgcggtatt tcgcgggatc
     1561 ggacatcgaa tggccgcgat agcgataggt ctgcatctcg acgatgtaag ggcccttgcc
     1621 cgagcgcgca tagtcgagcg cctcctcggc ggcctgcttg acggcgacca cgttcatgcc
     1681 gtcgacctga tggcccggga tctcgaaggg cgcgccgcgg ctcaccaggt cgtgcgacgc
     1741 cgagctgcgc gtgatcgccg tgcccatgcc gtagcggttg ttctcaatga tgtagacgac
     1801 cgggagcttc cacagcgccg ccatgttgaa gctctcatag acctggccct gattcatcgc
     1861 gccgtcgccg aggaaggtga tgctcacctt gccgtcctcg cggtatttgt gcgcgaagcc
     1921 caggccggtg ccgatcggca cctgggcgcc gacgatgcca tggccgccga agaagttctt
     1981 ctcgggggag aacatgtgca tcgagccgcc cttgcccttg ga
//
LOCUS       DASYNS010000045        10240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4692640, whole genome shotgun sequence.
ACCESSION   DASYNS010000045 DASYNS010000000
VERSION     DASYNS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10240
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4692640"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VGP50_01395"
     CDS             <1..1050
                     /locus_tag="VGP50_01395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8026049.1"
                     /translation="PEDARALNRAIGQVEDETEFLGEPGEYLSWADGFAEHLHAQRAR
                     QSGIHLMATHGDAIIGFLAAFAGGVERERGAIFIAGVGLRRSWRGRGVGTRLFRAIED
                     WARARNAWRLELRVDTENEHGLALYRKRGFVVEGKIADAFHVDGDRREHFLMGKPLRA
                     LDAPSWSPLELEPTSSSDPGTLRFGRLRPEEAPLLCRWERQLLAETPFLLKQPQEVLD
                     ETVMAKAIGAEPARTDRFSLAAVSAANGSEAVVGYGSAWKEAPRRMRHDAFVSVNVLR
                     SHWRQGIGGTLAAHIGSWARENGVSRLTMAITGHNRGGLRFAEAHGFKIEATSPRYAV
                     IDGRAVDRLRLAKLL"
     gene            1137..2396
                     /locus_tag="VGP50_01400"
     CDS             1137..2396
                     /locus_tag="VGP50_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV8026050.1"
                     /translation="MDRYWTERLSEFGATVTASEIRELLKVAADPDIISFAGGIPDPR
                     LFPFDEIREAHRKIFASNSLRLDAFQYAISEGYLPLRQWIGQHMGERGVAAAPENILI
                     TNGSQQGLDFVARLLLRRGDAVVVARPSYLGALQTFSGYGARLLAVPMDDQGMLPGPL
                     EAALQQHPKLLYLVPDFANPSGVTTTAPRRAELVALARRYGVPVVEDSAYERLRYEGD
                     LLPPIIALDQQERSRQAGAPTDNAIFLGTFSKIIAPAFRVGWVIAPPALTEKLVLMKQ
                     ASDLHSSTINQIVLAEVLPQAFQRQVDKIIPVYRARRDTMLKALDAHFPAGTTWTRPA
                     GGMFIWVDLPACIDAAMLLETALTESHVAFVPGAAFHADRSGKNTLRLSFSTSDTPQI
                     ETGIKRLGALLRRSIAAAATEPACDPP"
     gene            2646..3086
                     /locus_tag="VGP50_01405"
     CDS             2646..3086
                     /locus_tag="VGP50_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026051.1"
                     /translation="MRKTLAVIGTCFVFMLSYVGLASAQSKTPDAVIELSGGSVAAGI
                     GFTWGSGTLIYQGKRYPLSVSGVSLASVGAAGYTAAGTVYGLKSPQDITGVYTAVAAG
                     ATLGGGGDFSAMRNQNGVVIQMTATTEGLSLTLATEGMKIAVAN"
     gene            3123..4271
                     /locus_tag="VGP50_01410"
     CDS             3123..4271
                     /locus_tag="VGP50_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8026052.1"
                     /translation="MIVEAVTSANAFVVTSAAPGTVTLTVSGDWSIRHGLPSLVELDR
                     LLKPPGKVRRVLLKAAGLGHWDSALTAFLFAVAARCRDAGAVAEWSALPIDIRCLIEL
                     ASATPSHQNVAAPELRAFSLARVGAATLREKAALLRIIAFLGDTVIAFARLLRGRARY
                     RRADLVLLIEECGARALPIVSVISLLVGMIFAFVGAIQLKQFGADIYDANLVAVAMTR
                     EMAAVMTAVVLAGRTGAAFAAQIGSMQENDEVDALSTLGLSPIEFLVMPRLLALTLMT
                     PLLCVYSIALGIFGGFVVGVNVLDVTPITYLQRTQEAISMSDFGISMVKAAVFGWLVA
                     AAGCFCGIYSGRSASAVGVATTTAVVSGILAVIVADAVFAVLLNFLGI"
     gene            4274..5050
                     /locus_tag="VGP50_01415"
     CDS             4274..5050
                     /locus_tag="VGP50_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8026053.1"
                     /translation="MAIEAPAIAVESLTLGFGDEVVQRDVSFTIRRGEIFVIMGGSGS
                     GKSTLLRSMIGLLRPKTGQISYEGTPLWTTAGEALAQTQRRFGILFQSGALWSSLTLA
                     ENVGLPLSQYTTLRPPEIAAIVSLKLALVGLAGFEGYYPSEISGGMKKRAGLARAIAL
                     DPDILFFDEPSAGLDPLSSKRLDDLILELKDGLGATVVLVSHELASIMAIADNAVFLD
                     AESRTATAHGDPKRLRTGENPKVRDFLTRGRGLDEGEHPL"
     gene            5047..6057
                     /locus_tag="VGP50_01420"
     CDS             5047..6057
                     /locus_tag="VGP50_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV8026054.1"
                     /translation="MRASPTAIGAFTLGAVVLALAALVALGGGRFFTRHMRAVAYFTD
                     DLQGLSVGSPVDFRGVRIGRVAAIEIHLDVVKMNPVIPVYMDITTEFDIEDLKGKEDG
                     EVLRLAREQRLKVAIAHGLHAHLATQSLVTGLRFVELNFEPDAPRDFVGGDPSTVEIP
                     TSPSDLTQLQNALSRLPIDQIGESLSRLLGDADRLIASPEIPALLHALVASSDKIDRF
                     LDSTGTELGPLVANANETVKTARDTLLTAQSTLLEARTTFATGNRVMSTDLDDTLKAA
                     TGALQKAQKALADADGLLAANSPERYDFDQSLQNLSATTRALRLFSQDLERRPNAILL
                     GK"
     gene            6070..6732
                     /locus_tag="VGP50_01425"
     CDS             6070..6732
                     /locus_tag="VGP50_01425"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEV8026055.1"
                     /translation="MRVSLLLAVPRQVLPILFASLLVACQSEPSRLYVLNAAATPDRS
                     AILASADAPGRGHGAPPSPAHVLGVTVVLPQYLDTLNIVERVGANELKPNPDAQWGES
                     LSIDATRVVAENLGARLPSVEVIMLPSRMQRVPDYEVEIDLTRFESDRAGNALAKGRW
                     TISDDAGHEVASARVWRQEQVGGGGYDATAAAMSRILAAVSADIAAAAETLSARDSAA
                     RN"
     gene            complement(6749..7549)
                     /gene="ppk2"
                     /locus_tag="VGP50_01430"
     CDS             complement(6749..7549)
                     /gene="ppk2"
                     /locus_tag="VGP50_01430"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEV8026056.1"
                     /translation="MAKKARSRHDEADGESGHSYRKALIRLQIELVKLQRRVIAEGER
                     ILVIFEGRDAAGKDGTIKYITEHLSPRETRIVALGPPSDHERHSWYFQRYVPHLPAGG
                     EMVLFNRSWYNRAGVEPVMGFCTKAETREFLETVPAFEQMLVRSGIQLFKYYLDIDKQ
                     EQKRRLAQRRKDPLKQWKLSPIDMKAPKLWSAYSKARNEMFAKSHTPIGPWTIVRADD
                     KHLVRLNVIRDLLSRVEYRDRSPRLLPPDPKIVFSYDPSCLVNGMIAP"
     gene            complement(7627..7836)
                     /locus_tag="VGP50_01435"
     CDS             complement(7627..7836)
                     /locus_tag="VGP50_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026057.1"
                     /translation="MRHWEDEWQALVRERDAALARSQRIQDEITDSIRRRQPPAMRLM
                     RAADAAEAELAAIRARCRDFLQQLR"
     gene            complement(7935..8363)
                     /locus_tag="VGP50_01440"
     CDS             complement(7935..8363)
                     /locus_tag="VGP50_01440"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8026058.1"
                     /translation="MANDIIAQARSYWERIRGTRTMPRRGDLDPLDIPRLLPFVMLVD
                     VVTEPLDFRFRLIGSAIQAIVARNYVGQCFSELPHMARGNLIWAEYEAVVSQQRPVAA
                     ILDYVGSDRYVQGVRHGLMPLSNDDRAVNMIFAAVEIDRR"
     gene            8507..9508
                     /locus_tag="VGP50_01445"
     CDS             8507..9508
                     /locus_tag="VGP50_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026059.1"
                     /translation="MARRVVHPQPLATLRWLLLTGLILLAGAALGYLVWADKLDGERK
                     TNIAVSFVLTVLIASLLEWLPDLWRRFDGYLERGRFIRFFGRAALEDDVRLVFSHRVL
                     KKTVDAAPWDVYCKHIPPRDETPVPEGVKAWLSFQDLRGAACVAGEVAHQTGRVARFI
                     HDKDLDLQFDEIPYSAVAFGLGFNEFTHKLALFTERKLFAIEWGKSPRMGFGERTTDL
                     IRIPGSGLLNPPDDKDYAVLARIAPKQHDDRPDRYYFVCAGRTASGTAAAGRFLAYDW
                     PKLAALYKQHGRDPSRDSMVAVIEHAYHEETLTDLDQTARIATRGEQPWVIWHRIDA"
     gene            9738..10133
                     /locus_tag="VGP50_01450"
     CDS             9738..10133
                     /locus_tag="VGP50_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV8026060.1"
                     /translation="MSLSHSPEDVFRRLIEGVAARRWRELPDLYGEDAVVLHPFATDA
                     SARLVGREQLREHFARMATIDLEMRAHDVIVHTTGDPEVVVAEFAYHGRVGKDGRQFK
                     LPAVFVLRVRGGQIVESRDYLGPRQPLVS"
BASE COUNT         1645 a         3418 c         3339 g         1838 t
ORIGIN      
        1 cccgaggacg cgcgcgcgct caaccgggcg atcgggcagg tcgaggacga gacggagttt
       61 ctcggcgagc ccggcgaata cctgagctgg gccgatggct tcgccgagca cttgcatgcc
      121 cagcgggcgc ggcagagcgg catccatctg atggcgacgc atggcgacgc gatcatcggc
      181 ttcctcgcgg cctttgccgg cggcgtcgag cgtgagcgcg gcgcgatctt catcgccggc
      241 gtcggcctgc gccgctcatg gcggggccgt ggcgtcggca cgcggctctt ccgcgcgatc
      301 gaggattggg cgcgggcacg gaacgcctgg cggctcgagc ttcgcgtcga cacggagaac
      361 gagcacggcc ttgcgcttta ccgcaagcgc ggcttcgtgg tcgagggcaa gatcgccgac
      421 gccttccatg tcgacggcga ccggcgcgag catttcctca tggggaagcc gctgcgcgcg
      481 ctcgatgcgc cgtcctggtc tcccctcgaa cttgagccga cgtccagctc agatccgggg
      541 acgctgcgct tcggcagatt gcggccggaa gaagcgccgc tgctttgccg ctgggagcga
      601 cagttgctcg ccgagacgcc gttcctgctg aagcagccgc aggaggttct ggacgagacc
      661 gtcatggcca aggcgatcgg ggcggagccg gcacggacgg accgcttctc gctcgccgcc
      721 gtctctgccg cgaatggcag cgaggccgtg gtcggctatg gcagcgcctg gaaagaagcg
      781 ccgcggcgca tgcgccatga cgccttcgtg tcggtcaatg tgctgcgttc ccattggcga
      841 caaggaatcg gcggcacgct cgccgcgcat atcggcagct gggcgcggga gaatggcgtg
      901 tcgcggctga ccatggccat caccggacat aaccgcggtg ggcttcgctt cgccgaggcg
      961 catgggttca agatcgaggc gacatcgccg cgctacgccg tcatcgatgg acgcgccgtc
     1021 gaccgtctgc gcctggccaa gctgctttag ccatatgggt caatagtatt gacctaatac
     1081 gccggccgct gccatactcg gggcgcgcgg cccggatttc accggcagga tcgggcatgg
     1141 accgatattg gactgagcgg ctctcggaat tcggtgccac ggtcacggca tccgaaattc
     1201 gcgagctgct caaagtcgcg gccgaccccg acatcatttc tttcgcaggc ggcattcccg
     1261 atccccgcct cttcccgttc gacgagatcc gcgaggccca tcgcaagata ttcgccagca
     1321 acagcttgcg cctcgacgcc tttcaatacg cgatcagcga aggatatctg ccgctccggc
     1381 aatggatcgg ccagcatatg ggcgagcgcg gcgttgccgc cgcgcccgag aacattctca
     1441 tcaccaacgg ctcgcagcag ggcctcgact tcgtggcgcg gctgctgctg cgtcgcggcg
     1501 atgccgtcgt ggtcgctcgt ccgagctatc tgggcgcgct ccagaccttc tcgggctatg
     1561 gtgcgcgtct cttggccgtg cccatggatg atcagggcat gctgcccggc ccgctcgaag
     1621 cggcgctgca gcaacatccg aaactgctct acctcgtccc ggatttcgcg aacccgtcgg
     1681 gcgtgacgac cacagcgccg cggcgcgccg aactggtcgc gctggcgcgg cgctacggcg
     1741 tgccggttgt cgaggattcg gcctatgagc ggctgcgcta tgaaggtgac ttgctgccgc
     1801 cgatcattgc cctggatcag caggagcggt ctcgccaagc cggcgcgccg acggacaacg
     1861 ccatctttct cggcaccttc tccaagatca tcgcgcccgc cttccgcgtc ggctgggtga
     1921 tcgcgccgcc ggcgctcacg gagaagctgg tgctgatgaa gcaggcgagc gacctccact
     1981 ccagcacgat caaccagatc gtcctcgccg aggtgctgcc gcaggcattt cagcgtcaag
     2041 tcgacaagat catcccggtc taccgggcgc gtcgcgacac gatgctgaag gcgctcgacg
     2101 ctcatttccc cgccgggacg acatggacgc ggccggcggg cggcatgttc atctgggtcg
     2161 atttgcccgc ttgcatcgac gcggcgatgc tgctcgagac ggcgttaacc gagagtcacg
     2221 tcgccttcgt gcccggcgcc gcgttccatg cggaccgctc cggcaagaac acgctccgtc
     2281 tcagcttctc gaccagcgac acgccgcaga tcgagacggg catcaagcgt ctaggcgcgc
     2341 ttttgcggcg ctcgatcgcc gcggcggcaa ccgagcccgc ctgcgatcca ccctgagcgg
     2401 cagcttcgcc ctgacggtca ggtcgcggtg gctcggaagc gcgtcggctg cgccaccctt
     2461 cgggtcgaaa ccagagtata gccgcatcta accatctccc tatccgtccc ctcccaaggt
     2521 tcggtagctc tttacgcgcg tgcagtggcg aggggcgcga cccgcgtcgt agttttgttg
     2581 ggtcggctgc tacggccgcc ataggcgcgg caggtgccga tgctcaaata tttaggagat
     2641 cccttatgcg caagacgctc gctgtcatcg gaacctgctt cgtttttatg ctctcctatg
     2701 tcggtctcgc atccgcccag tcgaagacgc ctgatgccgt gatcgagttg tcgggtggtt
     2761 cggtcgcggc cggaatcggc ttcacctggg gaagcggcac gctcatctac caaggcaagc
     2821 gctatccgct ctcggtctcc ggcgtctcgc tggccagcgt cggcgcggcc ggatacaccg
     2881 ccgccggcac tgtctacggc ctgaagtcgc cgcaggacat caccggcgtc tacacggcgg
     2941 tcgccgccgg tgccacgctc ggcggcggcg gcgacttctc cgccatgcgc aaccagaatg
     3001 gcgtcgtcat tcaaatgacc gcaacgaccg agggcttgag cctgacgctc gcgaccgaag
     3061 gcatgaagat cgcggtcgcc aactgacgca accttcctta agatatcgcc gggctggtcg
     3121 ccatgatcgt ggaagcagtg acgtctgcga atgccttcgt ggtgaccagc gcggcgccag
     3181 ggacggtcac gctgaccgtc agcggcgatt ggtccatacg ccacggcctg ccatcgctgg
     3241 tcgagcttga ccgcctgctg aagccccccg gcaaggtgcg ccgcgtgctc ctcaaagcgg
     3301 cggggctggg gcactgggac agcgcgctga cggcgttcct cttcgccgtc gcggcgcgtt
     3361 gccgcgacgc cggggcggtg gcggaatgga gcgcgctgcc catcgatatc cgctgcttga
     3421 tcgagcttgc gagtgcaacg cccagtcacc agaatgtcgc cgcgccggag ctgcgcgcct
     3481 tctcccttgc ccgggtcggg gccgcaacac tgcgcgagaa agcggccttg ctccggatca
     3541 tcgcctttct cggcgacacg gtcatcgcct tcgcgcggct gttgcgcggc cgtgcgcggt
     3601 atcgccgggc ggatctggtg ctgctgatcg aggaatgcgg ggcgcgcgcc ctgccgatcg
     3661 tttcggtcat ctctctgctg gtcgggatga tcttcgcctt tgtcggcgcc atccaattga
     3721 agcagttcgg cgccgacatc tacgacgcga atctggttgc cgtcgccatg acacgcgaga
     3781 tggcggccgt catgaccgcc gtcgtgctcg ccggacgcac cggcgcggcc ttcgccgccc
     3841 agatcggctc gatgcaggag aatgacgaag tcgacgccct ctcgacattg gggctgtcgc
     3901 cgatcgagtt tctcgtgatg ccgaggctgc tggccctcac gctcatgact cctctgctct
     3961 gcgtctactc gatcgcgctt ggcatctttg gcggcttcgt cgtcggcgtg aacgttctcg
     4021 acgtcacgcc gatcacctat ctgcagcgga cgcaggaggc gatcagcatg agcgacttcg
     4081 gcatcagcat ggtgaaggcc gcggttttcg gctggctggt cgccgccgcc gggtgcttct
     4141 gcggcatcta ttccggccgg agcgcctcgg cggtcggcgt cgccaccacc accgccgtcg
     4201 tctcgggcat cctcgccgtc attgtcgccg acgcggtctt cgccgtgctg ctgaacttcc
     4261 tcggtatcta gttatggcca tcgaagctcc agcgatcgcg gtcgagagcc tcacgctggg
     4321 gttcggcgac gaggtggtgc agcgggacgt gagcttcacc attcgccgcg gcgagatctt
     4381 cgtcatcatg ggagggagcg gcagcggcaa gagcacgttg ctgcggtcga tgatcggcct
     4441 gctccgtccc aagacgggcc agatatccta tgaagggacc ccgctatgga cgacggcggg
     4501 ggaggcactc gcgcagacgc agcgccgctt cggcatcctc tttcagagcg gcgctttgtg
     4561 gagctcgctg accctcgccg agaatgtcgg cttgccgctg agccaataca ccacgctgcg
     4621 gccgcccgag attgccgcga tcgtatcgct gaagctggcg ctggtcggct tggcggggtt
     4681 cgagggctac tatccgtcgg agatcagcgg cggcatgaaa aagcgtgccg ggctcgctcg
     4741 cgccatcgcg ctcgatcccg acatcctgtt cttcgacgag ccctcggccg gactcgatcc
     4801 gctcagctcg aagcgtctcg acgatctcat cctcgagctc aaggacgggc tcggggcgac
     4861 ggtggtcctg gtctcccacg aactcgccag cattatggcg atcgccgaca atgccgtatt
     4921 tctggacgcc gagtcccgca cggccaccgc gcatggcgac cccaaacgcc tgcgaaccgg
     4981 agaaaacccg aaagtccgtg acttcctcac ccgcggccgt ggcctggacg aaggagagca
     5041 ccccctatga gagcgagccc tacggcgatc ggagctttca cgctgggagc ggtggtgctc
     5101 gcgctggccg cgctcgtcgc gttgggcggc ggccggttct tcacccggca catgcgcgcg
     5161 gtcgcctact tcaccgacga cctccagggc ctctcggtcg gatctcccgt cgactttcgc
     5221 ggcgtgcgga tcggccgggt ggctgccatc gagatccatc tcgatgtcgt caagatgaac
     5281 ccggtcatcc ccgtctacat ggacatcacc accgagttcg acatcgaaga cctcaaaggg
     5341 aaggaggacg gcgaggtgct caggctcgcg cgcgagcagc ggctcaaggt ggcgatcgcg
     5401 catggccttc acgcgcatct tgcgacgcag agtctggtca cgggcctacg cttcgtcgaa
     5461 ctcaacttcg agcctgacgc cccgcgtgac tttgtcggcg gagatccgtc gacggtcgag
     5521 attccgacga gcccgtccga cctcacgcaa ttgcaaaacg cgttgtcgcg gcttccgatc
     5581 gaccagatcg gcgaaagcct gtcgcgcttg ctgggcgacg ccgaccgcct catcgcctcg
     5641 cccgagatcc cggcgctgct gcacgcgctc gtcgcttcga gcgacaagat cgatcgtttt
     5701 ctcgattcga caggaaccga gcttggcccg ctggtcgcca atgcgaacga aacggtgaag
     5761 acggcgcgcg acaccctgct gacggcgcag agcacgttgc tggaggcgcg cacgaccttc
     5821 gcgaccggga accgcgtgat gtcgaccgat ctcgatgaca cgctcaaggc ggcgaccggc
     5881 gcactgcaaa aggcgcaaaa agcgctggcg gacgccgatg gtttgctggc cgccaattcg
     5941 ccggaacgct acgacttcga tcagagcttg cagaacctct cggcgacgac gcgggcgttg
     6001 cgcctcttct cccaagacct cgagcgccgc ccgaacgcga ttctcctggg aaaataggga
     6061 actcggccca tgcgcgtatc cttgcttttg gcggtgccgc gccaagtatt accgatcctc
     6121 ttcgcttcgc tcctggtcgc ctgccagtcc gagccttctc gcctctatgt gctgaatgct
     6181 gccgcgactc ctgatcggtc ggccatcctt gcttcggccg atgcgcccgg ccgcggacac
     6241 ggcgccccgc cgagcccggc gcatgtgctt ggcgtgacgg tggtccttcc gcaatatctc
     6301 gacacgttga atatcgttga gcgcgtcggt gccaatgagc tgaagccgaa ccctgatgcg
     6361 caatggggcg agagtttgtc gatcgatgcg acccgcgtcg ttgccgagaa tctcggggcg
     6421 agattgccct cggtcgaggt catcatgctg ccctcgcgca tgcaacgcgt gccggactac
     6481 gaagtcgaga tcgatctcac cagattcgag agcgaccgtg ccggcaacgc gctggcaaaa
     6541 ggacgctgga cgatcagcga tgacgccggt cacgaggtcg cgagcgcgcg tgtctggcgc
     6601 caggagcaag taggcggcgg cggctacgac gcaaccgccg cggccatgag ccggatcctt
     6661 gcggcggtca gcgccgacat cgccgcggcg gccgagacgc tatcggcacg cgacagcgct
     6721 gcgagaaact gatccagaag gccgcccgct aaggggcgat catgccgttg acgaggcagc
     6781 tcggatcgta gctgaagacg atcttgggat cgggaggcag caggcgcggg ctgcggtccc
     6841 ggtactcgac gcgcgacagg agatcgcgga tcacgttgag ccggacgagg tgcttgtcgt
     6901 cggcgcggac gatcgtccac ggcccgatcg gcgtgtggct tttcgcgaac atctcgttgc
     6961 gcgccttgct gtaggcgctc cagagcttcg gcgccttcat gtcgatcggg ctgagcttcc
     7021 actgcttcag cggatccttg cggcgctggg cgagccggcg cttctgttcc tgcttgtcga
     7081 tgtcgaggta atatttgaag agctgaatgc ccgagcggac cagcatttgc tcgaaggccg
     7141 gaaccgtctc gaggaattca cgcgtctcgg ccttggtgca gaagcccatc accggctcga
     7201 cgccggcgcg gttgtaccag ctgcgattga acagcaccat ctcgccgccg gccggcagat
     7261 gcggcacata gcgctggaaa taccaggaat ggcgctcgtg gtcggagggc gggcccagcg
     7321 ccacgatgcg ggtctcgcgc ggcgagagat gctcggtaat gtacttgatc gtcccgtcct
     7381 tgccggccgc gtcccgcccc tcgaagatga cgaggatgcg ctcgccctcg gcgatgaccc
     7441 ggcgctgcag cttgaccagc tcgatctgca gccggatgag cgccttgcga taggagtgcc
     7501 cgctttcgcc gtcggcctcg tcgtgccgcg atcgggcttt cttcgccatc cttgcttcct
     7561 cccctcgaga gacagccgag gattgccggc cgaggccggc gctggcaaga gtgtctttgg
     7621 cccggatcag cggagctgct ggaggaagtc gcggcaccgc gcccggatgg cggcaagttc
     7681 tgcctcggcg gcgtcggcgg cgcgcatcag gcgcatggcg ggaggctggc ggcgccggat
     7741 cgaatcggtg atctcgtcct ggatccgctg cgaacgcgcc agggccgcat cgcgctcgcg
     7801 cacgagcgcc tgccactcgt cctcccaatg ccgcatcgct tctccccgag cgcggcgtgc
     7861 tggctgcaca tgacgctagg acattcgcca cggcggggct cgtgagcgat ctgtgagcgc
     7921 gccgcggctc gggctcagcg gcggtcgatc tccacggcag cgaagatcat gttcacagca
     7981 cgatcgtcgt tcgacagcgg catgaggccg tgacggaccc cctgcacata gcggtcgctg
     8041 ccgacatagt cgaggatggc ggcgaccggc cgctgctgcg agaccacggc ttcgtactcc
     8101 gcccagatca agttgccccg cgccatatgc ggcagctccg aaaagcactg gccgacatag
     8161 ttgcgggcga cgatcgcctg gatggcgctg ccgatcagcc ggaacctgaa atcgagcggc
     8221 tccgtcacca cgtcgaccag catgacgaag ggcaggaggc gcggaatgtc gaggggatcg
     8281 agatccccgc gccgcggcat ggtgcgagtg ccgcggatgc gctcccaata gcttcgggct
     8341 tgcgcgatga tgtcattcgc catggctgag tgcatgcggc ggccattaaa gcagaggcgc
     8401 cgcgtttgcg ccaatccgcc gagcgctccg gggtgtgatc cagctcacac tgcgatggaa
     8461 cctgggaacc ctacaatcaa cggttgccga cgcctcctcg agaaccatgg cacgccgggt
     8521 cgttcatcct cagccgcttg cgacgctcag atggctgctg ctcaccggcc tcatcctgct
     8581 cgccggcgcg gcgctcggct atctggtctg ggccgacaag ctcgacggcg agcgcaagac
     8641 caacattgcc gtgagcttcg tgctgacggt cctgatcgcc tctcttctcg agtggctgcc
     8701 ggacctctgg cggcgcttcg acggctatct cgagcgcggc cgcttcatca ggtttttcgg
     8761 gcgcgccgcg ctcgaggacg atgtccgcct ggtcttctcc catcgcgtgc tgaagaagac
     8821 cgtcgacgcc gctccctggg acgtctactg caagcatatc ccgccgcgcg acgagacgcc
     8881 ggtgccggaa ggcgtcaagg catggctctc gttccaggac ttgcgcggcg cggcctgcgt
     8941 cgccggcgag gtcgcccatc agacgggccg cgtcgcgcgc ttcatccacg acaaggacct
     9001 cgatctgcaa ttcgacgaga tcccctattc cgccgttgcg ttcggcctcg gcttcaacga
     9061 gttcacccac aagctcgcgc tgttcaccga gcggaagctc ttcgccatcg agtggggcaa
     9121 gtcgccgcgg atgggtttcg gcgagcggac gaccgacctc atccgcattc ccggcagcgg
     9181 cctgctcaac ccgcccgacg acaaggacta tgccgtgctg gcgcgcatcg cgcccaagca
     9241 gcacgacgac cgtcccgacc gctactactt cgtctgcgcc ggccgcaccg cctcgggcac
     9301 cgcggccgcg ggacggttcc tcgcctatga ctggccgaag ctcgccgcgc tctacaagca
     9361 gcacgggcgc gatccgtcgc gcgactcgat ggtcgccgtc atcgagcacg cctatcacga
     9421 ggagacgctg accgacctcg atcagaccgc gcgcatcgcc acgcgcggcg agcagccctg
     9481 ggtgatctgg caccgcatcg acgcgtaggg ctgagccctg cgcttcggct cccttcggac
     9541 ggacggcttt gggtgggagg cgggaggcgg accttcagct tctgcacggc ggatcggcgc
     9601 tgtcgcctgc acgcgggtca cgcttcatcg tctgcgcgcc cagccatcgc tgcttacttg
     9661 tgagcaggtc ccgctggaaa taagcttcaa attccgcggg gcgcgcgcct cgtccccgat
     9721 aatcaaggca ggactccatg agtttatccc atagcccgga agacgtgttc cgcaggctga
     9781 tcgagggcgt ggcagcgcgg cggtggcgcg aacttcccga tctatacgga gaggatgcgg
     9841 tcgtactaca tccgtttgcc acagacgcat cggcgcggtt ggttggtcga gagcagttga
     9901 gggaacattt tgccagaatg gccacgatcg acctcgagat gcgcgctcac gacgtcattg
     9961 tgcacacgac cggcgatcct gaagtggtcg tcgcagagtt cgcctatcac ggtcgcgtgg
    10021 gcaaggacgg tcgccaattc aaattgccgg ccgtctttgt gctcagggtt cgaggcggtc
    10081 agattgtcga gtcacgcgac tatctgggcc cgcgacaacc gcttgtttca tagcgttgag
    10141 gtatatcctg tcgggtcgcg agcgctctaa cccttcaaaa cctctgcgca gcggtactaa
    10201 tagcccgttc cgtgcgcgaa atcacccgct gcagacgacc
//
LOCUS       DASYNS010000046         5417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1729401, whole genome shotgun sequence.
ACCESSION   DASYNS010000046 DASYNS010000000
VERSION     DASYNS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5417
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729401"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1908)
                     /locus_tag="VGP50_01455"
     CDS             complement(<1..1908)
                     /locus_tag="VGP50_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEV8026061.1"
                     /translation="MGAFSPAPRVLAVLGPTNTGKTHLAIERMLGHSSGMIGFPLRLL
                     ARENYDRVVRIKGPRAVALITGEEKIVPPNPAYFVCTAESMPLDRLVDFLAVDEIQLC
                     GDAERGHVFTARLLHARGRQETMFLGADTIRPLLRRLVPEAEFVTRPRFSTLRYLGPK
                     KVTRLPPRSAVVAFAVADVFALAELMRRQRGGTAVVLGALSPRARNAQVGMFQAGEVD
                     YLVATDAIGMGLNMDLDHVAFARLVKFDGAAQRHLSPAEIAQIAGRAGRHMSDGTFGT
                     TSELGPMSPEVVEAVENHRFDPLARLFWRNNRLDFASPAALLRSLEERPREPGLVAAR
                     EADDHLALRTLARQPDIAALAHHRTAVRLLWEVCQIPDFRKVMSDTHAKLLGQIYRHL
                     AGPKERLPIDWVARQVERLDRDDGDIDTLMTRIAHIRTWTYIGHRGEWLADAAQWQER
                     ARAIEDRLSDALHDRITQRFVDRRSAFLVKQLASEKDLLASVSHAGDVKVEGHYVGRL
                     DGFRFLPDEQAAGGETKTLLAAANRVLRGEIAVRARRLAAAPDGEFALTPLGCVTWHG
                     GTVGRLSRGDSALLPRVEPLPGDFLEGDLRDGVRRRLASFVRDALRHGLAPLFRARDA
                     TLAPAARGLAFQ"
     gene            complement(2055..2558)
                     /locus_tag="VGP50_01460"
     CDS             complement(2055..2558)
                     /locus_tag="VGP50_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV8026062.1"
                     /translation="MSYVKKVLLPGETLVYETGIHWLVYGRAILLLVAGAALAIGTMA
                     LTGGHIALPGPEAALAAAGLLGVVGFIAYVGAAIRRASTELAVTDQRVIYKSGVFARH
                     TLEMNRSKVESVDVDQSILGRVFGYGTVVLRGTGGSSEPMDRISDPLAFRSHITADPV
                     RRGANVE"
     gene            2708..4573
                     /gene="htpG"
                     /locus_tag="VGP50_01465"
     CDS             2708..4573
                     /gene="htpG"
                     /locus_tag="VGP50_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEV8026063.1"
                     /translation="MAPEKRSFQAEVSRLLEIVAHSLYSEKQIFLRELISNASDACDR
                     LRYAALTKPELIAGDSELRVELTPGKLNRTLTIADNGIGMNHEELVDNLGTIARSGTS
                     AFMQQLSGDARKDMALIGQFGVGFYSAFMVADKVEVLSQKAGEDQAWLWVSDGKGEFT
                     VEPAQKGGRGTTITLYLHADEAEYTDPGRLRQIVTTYSDHVALPIVLDSGGKKETLNT
                     AAALWTRAKSEITPEQYREFYHHAAHSFDEPWLTLHNKAEGTYQYTTLLFVPGAKPFD
                     LFDPARKAHVKLYVRRVFITDDCPEILPSYLRFLRGIVDSEDLPLNVSREMLQKNPML
                     AKMRGQITKRVLGEFAKKAKEAADDYGKFWDNFGAVLKEGLYEDRENQEALLPLVRFH
                     STAGDGLVSFDDYVARLKPGQEAIYTITGDNIEVLRKSPQIEGYRAKGVEVLLLTDPI
                     DEFWLPVLGKYKDHPLKSVTRGAADLGKIAAPEDRKEQPEPAKDSAVASVIALFKLSL
                     GDAVKDVRASDRLTDSAVCLVADEGDLDMHLERLLKQHNQRSDTAKRILEINPRHPLV
                     ARLAVLVGKDGAADALGDFAWLLLDQARILEGEQLPDPSGFAQRMSMVLERGLTG"
     gene            complement(4581..5309)
                     /locus_tag="VGP50_01470"
     CDS             complement(4581..5309)
                     /locus_tag="VGP50_01470"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEV8026064.1"
                     /translation="MKRAFVLASLLMTAAALAARAGESRSAPVVVELFTSQGCSTCPP
                     ADAYLGELASRPGVIALAFHVDYWNYIGWTDPFASKAATDRQRSYAKQLGLRYVYTPQ
                     MVINGTMEGVGSGREAITQLIADAAADKTPHVGVSVTRGAGGQILVHIEAGQTAEPAT
                     IWLVGFDREHTTQVPRGENEGRVLKDYQVVRSFKEIGSWSGESLDLALGENAATGDGG
                     VGVLVQLKGTGRILGAGCVKTPTS"
BASE COUNT          954 a         1837 c         1810 g          816 t
ORIGIN      
        1 ttgaaatgcg agaccccgcg ccgccggcgc cagcgttgca tcgcgcgccc ggaacaaggg
       61 cgcgaggcca tggcgcaacg cgtcgcgcac gaaggacgcg agacggcggc ggacgccgtc
      121 gcgcagatcg ccctcgagga agtcgcccgg cagcggctcg acgcgcggca gcaacgcgct
      181 gtcgccccgc gacaaccggc cgaccgtccc gccatgccag gtgacgcagc ccaggggcgt
      241 cagcgcgaac tcgccatcgg gtgccgccgc caaccgccgc gcgcgcaccg cgatctcgcc
      301 gcgtagcacg cgattggcgg ccgccagcag ggtcttggtc tcgccacccg ccgcttgttc
      361 gtcgggcagg aagcggaagc cgtcaagccg gccgacatag tggccctcaa ccttcacgtc
      421 gcccgcatga ctgaccgagg ccagcagatc cttctccgag gcgagttgct tcaccaggaa
      481 agccgagcgg cggtcgacga agcgctgggt gatgcgatcg tgcagcgcgt cggaaagccg
      541 gtcctcgatg gcgcgcgccc gctcctgcca ctgcgccgca tcggcgagcc attcgccgcg
      601 atggccgata taggtccagg tgcggatatg ggcgatgcgc gtcatcagcg tgtcgatgtc
      661 gccgtcgtcg cggtcgagcc gctcgacctg ccgcgccacc cagtcgatcg gcagccgctc
      721 cttgggccct gcgagatggc ggtagatctg ccccaggagc ttggcatgag tgtcgctcat
      781 caccttgcgg aaatccggga tctggcacac ttcccagagc agccgcacgg cggtgcggtg
      841 atgggcgagc gccgcgatgt cgggttgccg cgccagcgtg cgcaacgcca ggtgatcgtc
      901 ggcctcccgc gcggcgacga gacccggctc ccgcggccgc tcctcgaggc tgcgcagcaa
      961 ggccgcgggg gaggcgaaat cgagccggtt gttgcgccag aagagccgcg ccaggggatc
     1021 gaagcggtgg ttctccacgg cttcgaccac ctcggggctc atgggcccga gctcggaggt
     1081 ggtgccgaag gtgccatcgc tcatatggcg gccggcacgc ccggcgatct gcgcgatctc
     1141 ggccggcgag aggtggcgct gcgcggcgcc gtcgaatttc acgagacgcg cgaaggccac
     1201 atggtcgagg tccatgttga gccccatgcc gatcgcgtcg gtcgccacga ggtagtcgac
     1261 ctcgccggcc tggaacatgc cgacctgggc attgcgcgcg cgcgggctca aggcgcccag
     1321 cacgaccgcg gtgccgccgc gctggcggcg catcagctcc gccaaggcga acacgtcggc
     1381 cacggcaaaa gcgacgacgg cgctgcgcgg cggcagcctt gtcacctttt tggggccgag
     1441 atagcgcaag gtggagaagc gcgggcgcgt gacgaactcg gcctccggca cgaggcggcg
     1501 gagcagcggc ctgatggtgt cggcgccgag gaacatcgtc tcctggcgcc cgcgcgcatg
     1561 gaggaggcgc gccgtgaaga catggccgcg ctcagcatcg ccgcagagct ggatctcgtc
     1621 gacagccagg aagtcgacca ggcggtcgag cggcatcgac tcggcggtgc agacgaaata
     1681 ggcggggttc ggcgggacga tcttctcttc gccggtgatg agcgccacgg cgcggggccc
     1741 cttgatgcga accacccggt cgtagttctc gcgggcgagg aggcggagcg ggaagccgat
     1801 catgccgctc gaatggccga gcatgcgctc gatggcgaga tgcgtcttac ccgtgttggt
     1861 ggggccgagg acggcaagaa cgcgtggggc cggtgaaaaa gcgcccatcg ccctagaatc
     1921 gggacttctc cggcgaaagg caagcactgc cgcgaatttt cgcgcatccg gccggcacgc
     1981 cggcggattg gggcggctcc tgccgcccgg gcggcttgcc gtccatgacc gagaggaggg
     2041 ggtagcctca ggcgctattc gacattcgcg ccgcgccgca ccggatcggc cgtgatgtgg
     2101 ctgcggaagg cgagcgggtc gctgatgcgg tccatcggct cgctcgagcc gccggtgccg
     2161 cgcagcacga ccgtgccgta gccgaagacg cggccgagga tcgactggtc gacatcgacg
     2221 ctctcgacct tcgagcggtt catctcgagg gtatggcggg cgaagactcc ggacttgtag
     2281 atgacccgct gatcggtgac ggcgagttcg gtgctcgccc gtcggatcgc cgcgccgaca
     2341 taggcgataa aacccacgac tccgagcagc cccgctgccg ccagagctgc ctcggggccc
     2401 ggaagtgcga tgtggccgcc ggtcaaggcc atggtgccga tggccagggc ggccccggca
     2461 accagcagca ggatcgcccg cccatagacc agccagtgga tccccgtctc atagaccagg
     2521 gtctcgcccg gcaggaggac tttcttgacg tagctcatcg gcatgcgctc ctggaggcgg
     2581 ccactctcgg acccatcacc ctacaacacc cgaatggcat gtctctccgc cgggagcaac
     2641 aggctcttgc atggcaaggc cggcttgcat atctctgcgc cagtcgcatc aaccggtcca
     2701 acgagccatg gcgcccgaaa aaagaagctt ccaggcggag gtcagccgtc ttctcgagat
     2761 cgtcgcccac tcgctctaca gcgagaagca gatcttcctg cgcgagctca tctcgaacgc
     2821 gtccgacgcc tgcgaccggc tgcgctacgc ggcgctgacc aagcccgagc tgatcgccgg
     2881 cgacagcgag ctgcgcgtcg agctgacgcc gggcaagctc aaccgcaccc tcaccatcgc
     2941 cgacaacggg atcggcatga atcacgagga gctcgtcgac aacctgggca ccatcgcccg
     3001 ctcgggcacg tccgctttca tgcagcagct ctcgggcgac gcgcggaagg acatggccct
     3061 catcggccag ttcggcgtcg gcttctactc ggccttcatg gtggccgaca aggtcgaggt
     3121 gctctcgcag aaagcgggcg aggaccaggc ctggctctgg gtctcggacg gcaaaggcga
     3181 gttcacggtc gagccggcgc agaagggggg ccgcggcacc accatcaccc tctatctcca
     3241 cgcggacgaa gccgaataca ccgatcccgg gcgtctgcgc cagatcgtca cgacctattc
     3301 cgatcacgtg gcgttgccga tcgttctcga cagcggcggc aagaaagaga cgctcaacac
     3361 ggctgcggcg ctctggacgc gcgccaagtc cgagatcacg ccggagcagt atcgcgagtt
     3421 ctatcatcat gcggcgcaca gcttcgacga gccatggctc acgctgcaca acaaggcgga
     3481 agggacatac cagtacacga cgctgctctt cgtgcccggc gcgaagccct tcgacctctt
     3541 cgatcccgcg cgcaaggcgc atgtgaagct ctatgtacgc cgcgtcttca tcaccgatga
     3601 ctgcccggag atcctgccat cctatctgcg cttcctgcgg ggcatcgtcg attccgagga
     3661 tctgccgctc aacgtcagcc gcgagatgct gcagaagaat ccgatgctcg ccaagatgcg
     3721 cgggcagatc accaagcgcg tgcttggcga gttcgccaag aaggcgaagg aggcggcgga
     3781 cgactacggc aagttctggg acaatttcgg cgccgtgctc aaagaagggc tttacgagga
     3841 tcgcgagaac caggaggcgc tgctgccgct cgtccgtttc cactcgaccg ccggcgacgg
     3901 gcttgtgtct ttcgacgatt atgtcgcgcg cctgaagccg ggacaggagg cgatctacac
     3961 gatcaccggc gacaatatcg aggttctacg caagagcccg cagatcgagg gctatcgcgc
     4021 caagggcgtc gaggtgctgc tgctcaccga cccgatcgac gagttctggc tgcccgtgct
     4081 cggcaaatac aaggaccatc cgctcaaatc ggtgacccgc ggcgcggccg atctcggcaa
     4141 gatcgccgcc cccgaggacc ggaaagagca gccggagccg gcgaaggaca gcgccgtcgc
     4201 cagcgtgatc gcgctcttca agctctcgct gggcgacgcc gtcaaggacg tgcgcgcgtc
     4261 tgaccgcctc accgacagcg ccgtctgtct cgttgccgac gaaggcgacc tcgacatgca
     4321 tctggagcgg ctgctgaagc agcacaacca gcgttccgat accgccaagc gcatcctcga
     4381 gatcaatccg cgccatccgc tggtcgcccg gctggcggtg ctcgtcggaa aggacggcgc
     4441 ggcggatgcg ctcggcgact tcgcctggct gctcctggac caggcgcgca tcctcgaggg
     4501 cgagcagctg cccgatccca gcggcttcgc gcagcggatg tcgatggtgc tcgaaagagg
     4561 attgaccggc tgacggcggc tcagctggtc ggggtcttga cgcagccagc gcccaatatt
     4621 cggccggtgc ctttgagctg aaccagcacg cccacgccac catcgccggt cgcggcgttc
     4681 tcgccgagcg ccagatcgag cgactcgccg ctccagctgc cgatctcctt gaagctccgc
     4741 accacctggt agtctttgag gacgcggccc tcgttttcgc cgcgcggcac ttgcgtcgtg
     4801 tgctcacggt cgaagccgac gagccagatg gtcgcgggct cggccgtctg cccggcctcg
     4861 atatggacca ggatctgccc gcccgcgccc cgcgtcaccg acacgccgac atgcggggtc
     4921 ttgtcggcgg cggcatcggc gatgagctgc gtgatcgctt cacgccccga gcccacgccc
     4981 tccatcgtgc cgttgatcac catctgcggc gtgtagacgt aacgcaagcc aagctgcttc
     5041 gcatagctgc gctggcgatc ggtggccgct ttcgaggcga acggatcggt ccagccgatg
     5101 tagttccaat agtcgacatg gaaggcgagc gcgatcacgc cgggacgcga ggcgagctcg
     5161 cccagatagg cgtcggccgg cgggcaggtg ctgcagccct gcgaggtgaa cagctccacc
     5221 accacaggcg ccgaccggct ctcaccggca cgcgcggcga gcgccgccgc cgtcatcagc
     5281 agtgacgcga gaacgaacgc tcgcttcata cgagtaccca actgagcatt tgataatgat
     5341 gcgccagcac tgttcacggc gccagtcaaa caccttcaca atagggtgaa caccccaacc
     5401 attgaaggct aaaacat
//
LOCUS       DASYNS010000047         6020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_948457, whole genome shotgun sequence.
ACCESSION   DASYNS010000047 DASYNS010000000
VERSION     DASYNS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948457"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VGP50_01475"
     CDS             complement(<1..652)
                     /locus_tag="VGP50_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026065.1"
                     /translation="MKQDDLADLIDGIVDAGLAGAGEAALVGEFCRRAADIGSPIARV
                     TVVVDTLHPMLEGRAFRWRCDAPHETVVVEYGSTKEGEAADRWRRSAFYQLLSSGEAM
                     LRVRLAAPDAPDYPLLDELRAEGMVEYLALVHRFGAEGAIGEMDCVYTSWATALPAGF
                     SDRDVGALRRLSAALGLAVKSASLARIAQTLAETYLGRDPGRRVLGGAIARGIAEEI"
     gene            808..1680
                     /locus_tag="VGP50_01480"
     CDS             808..1680
                     /locus_tag="VGP50_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8026066.1"
                     /translation="MDRIALVTGATRNLGFALAQGLAQRLEPSDTVYLTGRDAGRVAE
                     SVRLLAGARAEVRGEMLDVAEPDAVEQFAASLAERHGGVDIVFSNHYSRVQPEDDPAE
                     VIDDYVAANNLGTTRVLRSFAPLLHDDGRLLVVASRAGSLRALAPVLHGRFQNLQSLD
                     DVDRALCAWRDDVRRGRAAGRAWPAWINIPSKIGQVAAVRVLAGQRRADDRRRGILIA
                     AISPGLIDTGASRAWLDLRGALTPEAVAGPLIDFALDPSPDPSFYGELVHVGREEPGP
                     FGTIMTSGSIVPWT"
     gene            1752..2156
                     /locus_tag="VGP50_01485"
     CDS             1752..2156
                     /locus_tag="VGP50_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV8026067.1"
                     /translation="MLVKRAEQAMVRAKSAALKAVGLTTAQYVALVELDHQPGVTAAT
                     LARACLVTPQAMMILLKTMEQQGLINRVSHPRHPNVLELHVTEVGREALQAARERVDP
                     IEQRVFDAFSPKDLAALRGLLSRFIAAFEKKS"
     gene            2274..2828
                     /locus_tag="VGP50_01490"
     CDS             2274..2828
                     /locus_tag="VGP50_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HEV8026068.1"
                     /translation="MRIKHGKFAALSLVAALAVEGCAAPPPGPTVFAVRGQNVSPDQF
                     QRDDGICQRYAYDVTARQAEASNDQALGAGVLGTVIGAGLGAVVGGGRGAAVGAVGGA
                     AVGGSVGANRSAWSNLPLQQRYNSLYLQCMQANGNGVPVFTPQPAVYYYVPSAPPPPP
                     PGYYPPPPPPPGYYPPPPPPPPGY"
     gene            complement(2838..4007)
                     /locus_tag="VGP50_01495"
     CDS             complement(2838..4007)
                     /locus_tag="VGP50_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV8026069.1"
                     /translation="MRTQWLVAGLCIVVLSQCSAIRQYQAQQKADEHKQKVQDALASC
                     RDLQADPRLDPIRGIVSIAGRPDLAMRSNPSFITEEQRPAVNALQSLVDQCRTILAKV
                     DPKLVGYSQEVSLDDARLRLYNRQMTIGEYNQARLEVAIELTERAAGVPAKTAAATGR
                     DAPSGSAPPDARRDGKSGPRPVSEGSGFVVSDGGHVLTNNHVVAGCSSIHTRLPDGTD
                     LVASVLAHDKINDLALLVLPTQPTQVASFRSGKVVRQGEPVVAVGFPLAPVLASGPNV
                     ATGSVAALAGAFDDSRYLQFSAPTQPGNSGGPLFDSAGNVVGVVSAKLDTATMVRAFG
                     DFPENVNFAIKNAVSTTFMEKNGVAYHNAPSTAAYTPADVAERAKAFTVYVECWN"
     gene            complement(4105..4683)
                     /locus_tag="VGP50_01500"
     CDS             complement(4105..4683)
                     /locus_tag="VGP50_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEV8026070.1"
                     /translation="MPRHEGGARPHHAGMIVFTIGHSTRSFDEFLALLREAGIECVVD
                     VRRFPMSRRHPQFNAEALAASLAAAGIGYRHVPALGGRRGKRADGKESPHTLWREESF
                     RNYADYAETEPFRSALDDLLCLAREKRVALMCAEAVWWRCHRRLIADYLLAAGVPVEH
                     ILDRGKIERAELTPGAQIGRDGAILYREARLV"
     gene            complement(4707..>6020)
                     /locus_tag="VGP50_01505"
     CDS             complement(4707..>6020)
                     /locus_tag="VGP50_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV8026071.1"
                     /translation="RASADNPRSDWYVWADARPDGTPPNNWLSVFGGSAWSWEPRRRQ
                     YYLHHFLAAQPTLDLRHPAVIEALLAAAEFWLARGVDGFRLDAIDFLLHDPELRDNPP
                     RAPADGVMPVKPFALQHHCHDMTQSEVPELLRRLRALADRRGGIALLGEVSSQEGAYE
                     RIERLTAHGDALHMAYTLRPLRLGDAGAALHEALAEIEAAGEGSGVCWAFSNHDVERA
                     ASRWSVSGDAEPGFAGLLMALLLSLRGSICVYQGEELGLSEAVLDVDDLRDPFGIAYH
                     PEFRGRDGARTPLPWNSEDDHAGFTSGTPWLPVPAEHKAQSIAAQEAEPDSPLRTWRR
                     LARWRKAHPALGAGELRVVAVPAPLIGFERISPAERLLLVFNPSDRAQRFALADLGAI
                     LPLEDHGLAMLVDGDVALLGPYGIFVAAVEAATAAQAGASGAGTR"
BASE COUNT          950 a         2109 c         2022 g          939 t
ORIGIN      
        1 cgatctcctc ggcaatgccg cgggcgatcg cgccgccgag cacgcgccgg ccgggatcgc
       61 ggccgagata ggtttccgcc agggtctggg cgatgcgggc gagcgacgcg gatttgacgg
      121 cgaggccgag ggccgcgctc agccgccgga gcgcaccgac gtcacggtcg ctgaaacccg
      181 caggaagagc cgtcgcccag gacgtgtaca cgcaatccat ctcgccaatc gcgccctccg
      241 cgccgaaacg atggacgagc gcgagatact ccaccatgcc ttcggcgcga agctcgtcga
      301 gcagcggata gtccggcgcg tccggcgcgg ccagccgcac tctgagcatg gcctcgcccg
      361 agctgagcag ctgatagaac gcactgcgtc gccagcgatc ggccgcctcc ccttccttgg
      421 tcgagccata ctcgacgacc accgtctcgt gcggcgcgtc gcagcgccag cgaaaggctc
      481 gcccttcgag catcgggtgc agggtgtcca cgaccaccgt cacgcgcgcg atgggactgc
      541 cgatgtccgc cgcacgccgg cagaactcgc cgacgagcgc ggcctcgccc gcgccggcga
      601 ggcccgcgtc gacgatcccg tcgatcaaat ccgccagatc atcttgcttc atgccgctca
      661 ctccagcccg gctccgcttc ccgccctatt tggtgcaccg cttccgccgc cgctacgggc
      721 cgttcgaggg tcatcgcggc gatgctcgtg gcggccggtc ttaagaatat acttgacgaa
      781 taccagtttg ttgatgacaa taagcctatg gaccggatag ctctcgtcac cggcgcgacc
      841 cgcaatctcg gctttgccct cgcccagggg ctggcccagc ggctcgagcc ttccgacacc
      901 gtgtatctca ccggacgcga tgccgggcgc gtcgccgagt ccgttcggct gctcgcgggc
      961 gcccgggcgg aagtgcgcgg cgaaatgctc gacgtcgccg aaccggacgc cgtcgagcaa
     1021 ttcgcggctt cgctggcgga gcgtcatggc ggcgtcgaca tcgtcttttc caatcactat
     1081 tcgcgcgtgc agccggagga tgacccggcc gaggtcatcg acgactacgt cgcggccaac
     1141 aatctcggca ccacgcgggt gctgcggagt ttcgcgcccc tgcttcacga cgatgggcgg
     1201 ctcctcgtcg tcgcaagccg ggccggcagc ctgcgcgcgc tggcgccggt gcttcacggg
     1261 cgctttcaaa acctccaatc gctcgacgac gtcgaccgcg ccctatgcgc ctggcgtgac
     1321 gacgttcgcc gcggccgcgc ggcgggccgg gcgtggccgg cctggatcaa cattccctcg
     1381 aagatcgggc aggtcgcggc ggttcgcgtt ctcgccggtc aacgccgcgc cgacgatcgg
     1441 cggcgcggca ttttgatcgc ggcgatctcg cccgggctga tcgacaccgg cgcatcgcgg
     1501 gcgtggctcg acctccgcgg cgccctcacg cccgaggcgg tggcgggtcc gctcatcgac
     1561 ttcgcgctcg acccctcgcc cgatccgtcc ttctatggcg agctcgtcca tgtcgggcgc
     1621 gaagagccgg gtccgttcgg taccatcatg acaagcggct ccatcgtccc ttggacatga
     1681 gcgatcggca aaaggcgtca aagcgcaggc ccgccgggcg cccatccgcg gacgaggagc
     1741 cgcgccttgg catgctcgtc aagcgcgccg agcaggcgat ggtccgggcc aagagcgcgg
     1801 cattgaaggc cgtgggtctg acgacggcac aatacgtggc actggtggag ctcgatcacc
     1861 agccgggggt cacggcggcg acgctggccc gcgcatgcct ggtcacgccg caagcgatga
     1921 tgatcctgct caagaccatg gagcagcagg gcctgatcaa ccgagtgtcg catccgcggc
     1981 accccaacgt gctcgagctg cacgtgacgg aggttggacg cgaggcgctt caggcggcgc
     2041 gggagcgcgt cgatccgatc gagcagcgcg tgttcgacgc cttctcgccc aaagatctgg
     2101 cggcgctgcg cgggcttctg tcacgcttca tcgcggcttt cgaaaagaag tcgtgaaaag
     2161 ccgtcgtttc gcgccgggtg ccgtgcggct atttggctgt cgcgcacggg gatgaatccg
     2221 cgcccgcggc ccttatatta ggcgggcttt tgcgtgaacg gagcgatcgg cagatgcgta
     2281 taaaacatgg caaattcgcg gcattgagcc tcgtcgcggc tctcgcggtc gagggctgtg
     2341 ccgcgccgcc ccccggcccg acggtgttcg cggtccgcgg gcagaatgtg tcgcccgacc
     2401 agtttcagcg cgacgacggg atctgccagc gatacgccta tgacgtgacc gcccgccagg
     2461 ccgaggccag caatgatcag gcgctcggcg ccggcgtcct cggcacggtc atcggcgcgg
     2521 ggctgggcgc tgtcgtcggc ggcgggcgcg gcgcggcggt gggtgccgtg ggcggcgccg
     2581 cggtcggcgg ctcggtcggc gccaatcggt cggcctggtc caatctgccg ctccagcagc
     2641 gctacaactc cctctacctg caatgcatgc aagccaacgg caacggcgtg ccggtcttca
     2701 caccgcagcc ggcggtctac tactacgtac cgtccgcgcc gcccccgccg ccacccggct
     2761 actacccccc gccaccgcct ccccccggct attacccgcc tccgccacca ccaccgcccg
     2821 gctattaacg gaacgcgtca gttccagcac tcgacataga ccgtgaacgc cttggcgcgc
     2881 tcggcgacat cggcgggggt atacgcggcg gtcgacggcg cattgtgata ggcaacgccg
     2941 tttttctcca tgaaggtggt cgacactgcg ttcttgatgg cgaaattcac gttctccgga
     3001 aagtcgccga acgcccgcac cattgtcgca gtgtcgagct tcgcgctgac gacgccgacc
     3061 acgttgccgg cgctgtcgaa gagcgggccg ccgctgttcc ccggttgcgt cggcgccgaa
     3121 aactggagat agcggctgtc gtcgaaggcg cccgcgagcg cggcaacgct gccggtcgcg
     3181 acattgggcc ccgatgccag caccggcgcc agtggaaagc cgacggccac gacaggttcg
     3241 ccttgtcgaa cgaccttgcc tgagcggaag gacgctacct gagtcggttg cgtcggcagc
     3301 accagcaggg ccaaatcgtt gatcttgtca tgggcgagga cggaagccac cagatccgtg
     3361 ccgtcgggca ggcgcgtatg aatcgaagag cagcctgcga cgacatgatt attggtcagc
     3421 acgtgcccgc cgtcgctgac aacgaagccc gagccttcac tgacgggccg cggtcccgat
     3481 ttcccgtcac gacgcgcgtc gggcggcgcg gagccagatg gcgcgtcgcg gcctgtcgcc
     3541 gcagcggtct tcgccgggac acctgcggcg cgctccgtga gctcgatcgc gacctccagt
     3601 cgtgcctgat tatactcgcc gatcgtcatc tgccggttgt agaggcggag ccgcgcatca
     3661 tcgaggctca cttcctgcga gtatcccacc aatttgggat ccactttggc caggatggtt
     3721 cggcattgat caacgagcga ctgcagcgcg ttgaccgccg gacgctgctc ctcggtgatg
     3781 aacgacgggt tcgaccgcat cgccaggtcc ggccggcccg cgatcgagac gatcccgcga
     3841 ataggatcga ggcgcgggtc ggcctggaga tcgcggcagg acgcgagagc atcctggact
     3901 ttctgcttgt gctcgtccgc tttctgctgc gcctgatact gacggatggc actgcactgg
     3961 gacaggacga cgatgcaaag tcccgcaacc aaccactgcg tgcgcatccg caaccctccg
     4021 ggctactttt gcgttagttt aattctttcc cagagcaagg caaccattgc gcgaacggcc
     4081 gcgacgccgg cgcgggactg gcggctaaac caacctcgcc tcgcgataga ggatcgcgcc
     4141 gtcgcgtcct atctgcgccc cgggcgtgag ctcggcccgc tcgatcttgc cgcgatcgag
     4201 gatgtgctcg accgggacgc cggcggcgag cagatagtcg gcgatgaggc ggcgatggca
     4261 gcgccaccac acggcttcgg cgcacatgag ggcgacccgc ttctcgcggg cgaggcacaa
     4321 gaggtcatcg agcgcgctgc ggaacggctc ggtctcggcg tagtcggcgt agttgcggaa
     4381 gctctcctcg cgccagagcg tgtgcggcga ttccttgccg tccgcccgct tcccgcgccg
     4441 cccgcccagc gccgggacat ggcgatagcc gatgcccgcc gcggccagcg acgcggcgag
     4501 cgcttccgcg ttgaattgcg gatggcgccg cgacatcgga aagcgccgca catcgacgac
     4561 gcattcgatc cctgcctcgc gcagcaaggc gaggaactca tcgaagctgc gcgtcgaatg
     4621 cccgatggtg aagacgatca tgcccgcatg atgtggtcgc gcgccgccct catgtcgagg
     4681 caccggaact ccaggaactc cacgccctaa cgcgtccctg ccccgctggc accggcctgc
     4741 gccgcggtcg cagcttcgac ggcggccacg aaaatgccat aggggcccaa caacgcgacg
     4801 tcgccgtcga ccagcatggc aaggccgtgg tcttcgagcg gcaggatcgc acccagatcc
     4861 gcgagcgcga agcgctgcgc ccgatcgctc gggttgaaca ccaggagcag ccgctctgcc
     4921 ggcgagatcc gctcgaagcc gatgagcggc gccggcacgg cgacgacacg gagctcgccc
     4981 gcgccgagcg ccggatgcgc cttccgccag cgcgccagcc gccgccaggt ccggagcggc
     5041 gagtccggtt ccgcctcctg tgccgcgatg ctctgcgctt tatgctcggc cggcaccggc
     5101 agccagggcg tgccgctggt gaagccggcg tgatcgtcct cgctattcca ggggagcggc
     5161 gtgcgggcgc cgtcgcggcc gcggaattcg ggatggtagg cgatgccgaa gggatcgcgc
     5221 aggtcgtcga catcgagcac cgcttcgctc aggcccagct cctcgccctg atagacgcag
     5281 atgctgccgc gcaggctgag cagcagcgcc atcagcaggc cggcgaaacc gggctcggca
     5341 tcgcccgaga cgctccagcg actcgccgcg cgctcgacgt cgtggttgct gaaggcccag
     5401 cagacgccac ttccctcgcc cgccgcttcg atctcggcga gcgcctcgtg cagcgccgcg
     5461 ccggcatcgc ccaagcgcag cggccggagc gtgtacgcca tgtgcagcgc gtcgccatgc
     5521 gccgtcagac gctcgatccg ctcataggcg ccctcctggc tcgacacctc gcccaggagc
     5581 gcgatgccgc cgcggcggtc ggcgagcgcg cgcaagcggc gcagcagctc cggaacttcg
     5641 ctctgcgtca tgtcgtggca atgatgctgg agcgcgaaag gcttcaccgg catgacgcca
     5701 tccgctggcg ctcgcggcgg attgtcgcgc agctcgggat catgcagcag gaaatcgatg
     5761 gcatcgagac ggaacccgtc gacgccgcgg gcgagccaga actcggccgc ggcgaggagt
     5821 gcctcgatca ccgccggatg gcgcaggtcg agcgtgggct gcgccgcgag gaaatggtgg
     5881 agataatatt gccggcgccg cggttcccag ctccaggcac tgccgccgaa gacggagagc
     5941 cagttgttgg gcggcgtgcc gtcgggcctg gcgtccgccc acacatacca gtcgctgcgc
     6001 ggattgtcgg cgctcgcgcg
//
LOCUS       DASYNS010000048         2838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_480919, whole genome shotgun sequence.
ACCESSION   DASYNS010000048 DASYNS010000000
VERSION     DASYNS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480919"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1992)
                     /gene="sppA"
                     /locus_tag="VGP50_01510"
     CDS             complement(211..1992)
                     /gene="sppA"
                     /locus_tag="VGP50_01510"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEV8026072.1"
                     /translation="MRRLVIGFFALIGVVVFGLLAVLVIVLIVAAPGTKRLAATDILT
                     IDLTQPLPDAPPGDGVERILLGERLTLRDVIDALARAGDDARIKGVVARVGEGALGTA
                     AIQELRDALAGFRAKGKFALAHADGFGELGSGTRDYYLAAAFDEVWLQPFADVGLIGL
                     RVEVPFFRGSLDKLGLVPRFDHRSEYKTAMNIFTETKMTPPHREETEAILNSVYGQLV
                     EGIAAGRKLDPDAVRHLVDQGPFGADEALAAHLVDHLGYREDAVAAARTRAGENAELV
                     SVSDYLQSAGRPHQSGPTIAVIYGNGLITRDGSSGSPLSGSQVSGADALSRAFRTAAE
                     DASVRAILFRIDSPGGSATASETIWRAVQHAKEAKKPVIVSMGEVAGSGGYYIAAPAD
                     RIVAEPATLTGSIGVVAGKVLIDGISEKLGVSWDAAQLGQQAGMFSVITDFSPAEHDR
                     FERMLDAIYAGFKQRVADGRNLSADAVEQVAKGRVWTGADAKERHLVDALGGFDTALA
                     LAKEVAGIPADSDVTLKLFPPPSNSPAAVLARLMGRDGDESGESTIAARLALQDPLLR
                     ELRPLLRDLELAAAPPGALIMPPLEVR"
     gene            complement(2030..>2838)
                     /gene="aroC"
                     /locus_tag="VGP50_01515"
     CDS             complement(2030..>2838)
                     /gene="aroC"
                     /locus_tag="VGP50_01515"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV8026073.1"
                     /translation="DARWKDYAAIKDSFRPGHADYTYWKKYGIRDYRGGGRASARETA
                     SRVAAGAIARKILGPGVTIRGAVIQIGPHKIARDRWDWAAVEDNPFWSPDRQMAQTWE
                     GFLDGVRKAGSSAGAVAEIVASGVPAGLGEPIYDKLDGELAKAMMSINAVKGVEIGAG
                     FAAAALSGEDNADEMRMKDGAVAFLSNQAGGILGGISTGQDIVVRFAVKPTSSILTPR
                     RSVDVHGRDIEVSTKGRHDPCVGIRAVPVGEAMMAVVLADHLLRHRALRG"
BASE COUNT          438 a          964 c          993 g          443 t
ORIGIN      
        1 tcggggcgcg gtcacaccca cctcatccta aatccttctc cgccctgaag ggagggcgga
       61 gaaggattta ggatgaggtg ggtgtgaccg cgccccgata ttgagggcgc gtgttcgcgt
      121 tcatctccgc agggccgaac atcccccacc tcaccccgcc ctctccgccc ccgagggcgg
      181 agagggagaa gaagcaacct gggcctggac ctatcgtacc tccagcggcg gcatgatgag
      241 ggcgcccggg ggggcggccg ccagctcgag gtcgcgcagc aagggccgca gctcgcgcag
      301 cagcggatcc tgcagcgcca gccgtgccgc aatcgtgctt tcgccgcttt cgtcgccgtc
      361 gcggcccatg aggcgggcga ggacggccgc gggcgagttg ctgggcggcg ggaagagctt
      421 caaggtgacg tcgctgtcgg cggggatgcc ggccacctcc ttggcgagcg cgagcgcggt
      481 gtcgaagccg cccagtgcgt cgacgaggtg gcgctccttg gcgtcggcgc cggtccagac
      541 gcggcccttg gccacctgct cgaccgcatc ggcgctcaaa ttgcgtccgt cggcgacgcg
      601 ctgcttgaag cccgcataga tcgcatcgag catgcgctcg aaacggtcat gctcggccgg
      661 cgagaaatcg gtgatgacgc tgaacatgcc ggcctgctgg ccgagctgcg ccgcatccca
      721 gctgacgccg agcttctcgg agatgccgtc gatcagcacc ttgccggcga cgacgccgat
      781 cgagcccgtg agtgtcgcgg gctcggcgac gatgcgatcg gccggcgccg cgatgtagta
      841 gccgccggag ccggcgacct cgcccatgct gacgatcacc ggcttcttcg cctccttggc
      901 gtgctgcacg gcgcgccaga tcgtctcgga ggccgtggcc gagccgccgg ggctgtcgat
      961 gcggaacagg atggcgcgta cggaagcgtc ctcggcggcc gtccggaaag cgcggctcag
     1021 cgcatccgcc ccggagacct gggagcccga gagcgggctt ccggaactgc cgtcgcgggt
     1081 gatgaggccg ttgccataaa tcacggcgat ggtcggtccg ctctggtgcg gccgtccggc
     1141 gctttgcaga tagtccgaca ccgagacgag ctcggcattc tcgcccgcgc gcgtccgcgc
     1201 ggcggcgacc gcgtcctcgc gatatccgag atggtcgacc agatgcgcag caagcgcttc
     1261 gtcggcgccg aaggggccct ggtcgaccag gtgccgcact gcgtcgggat cgagcttgcg
     1321 cccggcggcg atgccctcga ccagctgacc gtagaccgag ttgaggatgg cttcggtctc
     1381 ctcgcgatgc ggcggcgtca tcttggtctc ggtgaagatg ttcatcgcgg tcttgtactc
     1441 ggagcgatga tcgaagcgcg ggacgagacc gagcttgtcg aggctgccgc ggaagaaggg
     1501 cacttccacc cggagcccga tcagcccgac atcggcgaat ggctggagcc acacctcgtc
     1561 gaaagcggcg gcgaggtaat agtcgcgcgt ccccgagccg agctcgccga agccgtcggc
     1621 atgggcgagc gcgaattttc ctttggcgcg gaacccggcg agggcatcgc gcagctcctg
     1681 gatcgcggcg gtgccgagag cgccttcgcc cacgcgcgcc accacgccct tgatgcgcgc
     1741 gtcgtcgccg gcgcgggcga gcgcgtcgat cacgtcgcgc agcgtcaggc gctcgcccag
     1801 caggatgcgc tcgacgccgt cgccgggcgg cgcgtccggc aggggctgcg tcaggtcgat
     1861 ggtgaggatg tccgtagctg caagccgctt cgtacccggc gcggcgacga tcagcacgat
     1921 gacgagcacg gcgaggagac cgaaaacgac gacgccaatc aacgcgaaga aaccgatgac
     1981 gagacgacgc atgaaatact ccgattgtga cgctttcccg atcctttgct cagccgcgga
     2041 gcgcgcggtg gcgcagcagg tggtcggcca gaaccaccgc catcatcgct tcgccgacgg
     2101 gcacggcgcg gatgccgacg caaggatcgt gacggccctt ggtgctcact tcgatgtctc
     2161 tgccatgaac gtcgaccgag cggcgcggcg tcaggatcga gctcgtcggt ttcaccgcga
     2221 agcggacgac gatgtcctgc ccggtcgaga tgccgccgag gatgccgccc gcctgattcg
     2281 agagaaaagc gacggcgccg tccttcatgc gcatctcgtc ggcattgtcc tcgcccgaga
     2341 gcgccgccgc ggcgaagccg gcgccgatct ccacgccctt cacggcattg atgctcatca
     2401 tcgctttcgc aagctcgccg tcgagcttgt cgtagatggg ctcgccaaga ccggcgggaa
     2461 cgccgctcgc cacgatttcg gccacggcgc ccgccgacga gccggccttg cgcacgccgt
     2521 cgagaaagcc ttcccaggtc tgcgccatct gccggtcggg actccagaag ggattgtcct
     2581 cgaccgcggc ccagtcccag cggtcgcgcg cgatcttgtg cggcccgatc tggatgacgg
     2641 cgccgcggat cgtcacgccg ggcccgagga tcttgcgcgc gatggcgccg gcggcgacgc
     2701 gggatgccgt ctcgcgcgcc gaggcgcgcc cgccgccgcg gtagtcgcgg atgccgtatt
     2761 tcttccagta ggtatagtcg gcgtgacccg gacggaagct gtccttgatt gcggcgtagt
     2821 ccttccaccg tgcatcct
//
LOCUS       DASYNS010000049         3384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4381899, whole genome shotgun sequence.
ACCESSION   DASYNS010000049 DASYNS010000000
VERSION     DASYNS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4381899"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VGP50_01520"
     CDS             <1..1227
                     /locus_tag="VGP50_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV8026074.1"
                     /translation="RFDRLALVPSDIRNVDDLVAKWPVLDRAEMAEDAREHPPYGTYT
                     AMTDAVWTERGWMMFSSSGTTDRPRVFRYSHVDRELWAWANARALHAMELRRGDTVFV
                     VSGLGPHVWAWSVHSALMKMRLPWLSGGGMDAKARATVVLRYRPTILLCTPSYALYLA
                     RVMQEMGADPRTTSIRTVFVGGEPGVSVAATRERLQELWGARLVEFYGCTEAAPHAGG
                     FSCPASQPVQGPVSAHLLEDVQVWETVDPASHAALPEGRRGLTVCTNLHSESSPQLRF
                     LVGDYATYDRSICACGRTHVRAIGSFAGRADDLVNLRGIKMVPSLIEDVVRGVSGAGD
                     EFEIVLTTNPDGLDIMTVRIEHVEFPRADHVARAIADAIRTRCEVRVDVEVLGPGTLP
                     KTEFKAARVKDHRKKG"
     gene            complement(1379..2386)
                     /gene="hcrB"
                     /locus_tag="VGP50_01525"
     CDS             complement(1379..2386)
                     /gene="hcrB"
                     /locus_tag="VGP50_01525"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit beta"
                     /protein_id="HEV8026075.1"
                     /translation="MDALPELRLLRPQTLAEALAAREADGRAQLLGGGTDLIVNLRRG
                     IVAPPVLVDLTGIAELRGIEAEGDTLRVGAAVTLAELAAHPQVRAQYRVLAEAAASIA
                     GPTQREMGTVGGNLCLDTRCIYYNQSEWWREANDHCLKTTGTVCHVAPKSRGVCFATF
                     SGDLAPALLTLEGEVEIAGPTGRRTLPLGELYIGHARHDASTGDGKNYLALRPGEIVV
                     AARARRRPGLRSGYDKIRIRRSIEYPVAGVAVALRREGDALAELRVAITGTNPRPVLL
                     AGSDGLCGGALDDRVFAGLDALVRDQIMAMKTTFTPGHYRRRVAGVLAARLIRRLYES
                     L"
     gene            complement(2386..>3384)
                     /locus_tag="VGP50_01530"
     CDS             complement(2386..>3384)
                     /locus_tag="VGP50_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV8026076.1"
                     /translation="YISGASKPVNWTGEPHATVKLKLDFDGSIVLLTGAAEIGQGSST
                     VLVQSVAEVLGLDIARLRIVTGDSEVVPKDNGSYSSRVTFMVGNAAIDAARNLKRVLV
                     AAAARKLEARPDDIECLGELYRAGAQDKGVSFDEVVSEALKDSGTIIVTGNYSTIPES
                     HGGNKYRGAAIGGTMGYSYSAQVVEVTVDEDTGVVTVDKVWAAHDCGKALNRLTVEGQ
                     VQGSVWMGMGQAMSEETGYHDGLLLTGNMLDYRVPTIEDSPPIEVGIVESNDPHGPFG
                     AKEAGEGSLAAFLPALANAIADAVGLRFDTLPVTPDRVFAALEERVRARRASAKVA"
BASE COUNT          521 a         1218 c         1105 g          540 t
ORIGIN      
        1 cgcttcgacc ggctcgcgct cgttccgtcc gacatccgca acgtcgacga cctcgtcgcg
       61 aaatggccgg tcctcgaccg cgccgaaatg gccgaggatg cgcgcgagca cccgccctat
      121 ggcacctaca ccgcgatgac cgacgcggtc tggacggagc gcggctggat gatgttctcg
      181 tcctcaggga ctaccgacag gccgcgcgtc ttccgctatt cgcatgtgga tcgcgagctc
      241 tgggcctggg ccaatgcccg cgcgctccat gccatggagt tacgccgcgg cgacaccgtc
      301 ttcgtcgtca gcggcctcgg cccccatgtc tgggcctgga gcgtccattc ggcgctgatg
      361 aagatgcggc tgccctggct ctcgggcggc ggcatggacg ccaaggcgcg cgccaccgtc
      421 gtgctgcgct atcgcccgac gatcttgctc tgcaccccct cctacgcgct ctatctcgcg
      481 cgcgtcatgc aggagatggg cgccgacccg cgcacgacat cgatccgcac ggtcttcgtc
      541 ggcggcgagc ccggcgtgag cgtcgccgcc acgcgcgagc ggcttcagga gctgtggggc
      601 gcgcggctcg tggaattcta cggctgcacc gaagccgcgc cccatgccgg cggattctca
      661 tgcccggcct cccagcccgt gcaaggtccg gtctcggcgc atctcctcga ggacgtgcag
      721 gtctgggaga cggtcgatcc ggccagccac gcagcgctgc cggagggccg ccgcggcctc
      781 accgtctgca ccaacctcca ctccgaatcc tcgccgcagc tgcgcttcct cgtcggcgac
      841 tacgccacct acgaccgcag catctgcgct tgcggccgca cccatgtccg cgccatcggc
      901 tctttcgccg gacgcgctga cgatctcgtc aatctgcgcg gcatcaagat ggtgccctcg
      961 ctgatcgagg atgtcgtgcg cggtgtctcc ggcgccggcg acgagttcga gatcgtgctc
     1021 accaccaatc ccgacggcct cgacatcatg acggtgcgca tcgagcatgt cgaattcccg
     1081 cgcgccgacc atgtcgctcg cgccatcgcc gacgcgatcc gcacgcgctg cgaagtgcgc
     1141 gtcgacgtcg aagtgctcgg ccccgggacc ctccccaaga ccgaattcaa agccgcccgc
     1201 gtcaaggacc accggaagaa ggggtaaccg tcccgcggca tcagtgtcgt gcgtccttcg
     1261 acaagctcag gacgaggaag agcttggacg gcatcgaatt gtcgatgcca gaaagaacag
     1321 acctcatcct gagcttgtcg aaggacgcac gatcgcgttg cagcgcgatg tcgccgcgtc
     1381 agagactctc atagagccgg cgtatcagcc gcgcggcgag cacgccggcg acgcggcgcc
     1441 ggtagtggcc gggcgtgaac gtcgtcttca tcgccatgat ctgatcgcgc accagagcgt
     1501 cgaggccggc gaacacgcga tcatcgagcg cgccgccgca caggccatcg ctgccggcga
     1561 gcagcaccgg ccgcggattc gtccccgtga tcgcgacgcg gagctcggcc aaggcatcgc
     1621 cctcgcgacg gagcgccacc gcgacgcccg ccaccggata ctcgatcgag cggcggatgc
     1681 ggatcttgtc atagcccgag cgcaggccgg gccggcgccg ggcgcgcgcg gcgacgacga
     1741 tttcgcccgg gcgcagcgcc agataattct tgccgtcgcc cgtcgaagcg tcatgccgcg
     1801 catggccgat atagagctcg cccagcggca gcgtccgccg tcccgtcgga ccggcaatct
     1861 cgacctcgcc ctccagcgtc agcagcgccg gcgcgagatc gccgctgaat gtggcgaagc
     1921 acacgccgcg gctcttgggc gcgacatggc agaccgtgcc cgtggtcttg aggcaatgat
     1981 cgttggcctc tcgccaccat tcgctctggt tgtagtagat gcaacgcgtg tcgaggcaga
     2041 gattgccgcc gacggtaccc atctcgcgtt gcgtcgggcc ggcgatcgag gccgcggcct
     2101 cggccagcac ccgatactgc gcccgcacct gcggatgagc ggcaagctcg gccaaggtca
     2161 cggccgcacc gacgcggagc gtgtcgccct cggcctcgat gccgcgcagc tcggcgatgc
     2221 cggtgagatc gacgaggacc ggcggtgcga cgatgccgcg gcggagattg acgatgagat
     2281 ccgtgccgcc gcccaggagc tgcgcccggc catcggcttc gcgcgcggcc aacgcctccg
     2341 ccagcgtctg tggccgcaac agccgcagct cgggcaaggc gtccatcaag caacctttgc
     2401 gctcgcgcgc cttgcgcgca ctcgttcctc gagcgctgca aagacgcggt ctggcgtcac
     2461 cggcagcgta tcgaagcgca gcccgaccgc gtcggcgatg gcgttggcga gcgccggcag
     2521 aaacgcggcg agcgacccct cgcccgcttc tttcgcaccg aagggtccgt gcggatcgtt
     2581 gctctcgacg atgccgacct cgatcggcgg cgagtcctcg atggtcggca cgcggtaatc
     2641 gagcatgttg ccggtgagca gcaggccgtc gtgatagccg gtctcctcgc tcatcgcctg
     2701 gcccatgccc atccacaccg agccctgcac ctggccttcg acggtgaggc ggttcagcgc
     2761 cttgccgcaa tcatgcgccg cccagacctt gtcgaccgtg acgaccccgg tgtcctcgtc
     2821 gaccgtgacc tcgaccacct gggccgagta gctgtagccc atggtgccgc cgatcgcggc
     2881 gccgcgatat ttgttgccgc catgggattc gggaatcgtc gagtagttgc cggtgacgat
     2941 gatcgtgccg ctgtccttga gcgcttcgct caccacctcg tcgaaggaca cgcccttgtc
     3001 ctgggcgccg gcgcggtaga gctcgcccag gcattcgata tcgtccggcc gtgcctcgag
     3061 cttgcgcgcc gcggccgcca ccaggacgcg cttcaggttg cgcgccgcgt cgatcgcggc
     3121 attgccgacc atgaaggtga cgcgcgagga gtaggaaccg ttgtccttgg gcacgacctc
     3181 gctgtcgccg gtgacgatgc gcaagcgcgc gatgtcgagg cccagcacct cggcgacgct
     3241 ctgcaccagc acggtcgacg agccctggcc gatctcggca gcgccggtca acagcacgat
     3301 cgagccgtcg aaatcgagct tgagcttcac cgtcgcatgg ggctcgccgg tccagttgac
     3361 gggcttcgac gcgccgctga tgta
//
LOCUS       DASYNS010000050         4680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3601485, whole genome shotgun sequence.
ACCESSION   DASYNS010000050 DASYNS010000000
VERSION     DASYNS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3601485"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..579
                     /locus_tag="VGP50_01535"
     CDS             151..579
                     /locus_tag="VGP50_01535"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HEV8026077.1"
                     /translation="MLVARLALATAAAFAGAALYVNVAEQPARLGLDDRALLAQWKPS
                     YSRGAAMQASLAAISGLLGLAAFWLSGDWRWLIGAALILANWPYTLLCIMPTNNRLKA
                     IADDAAGAASRALIERWGWLHAARTALGIAATAAYLWALS"
     gene            complement(604..1857)
                     /locus_tag="VGP50_01540"
     CDS             complement(604..1857)
                     /locus_tag="VGP50_01540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8026078.1"
                     /translation="MTAEVSTSARFPAARTAELSRRRVAIGFINWAHALDHYVMLIFP
                     TVVIELEAVYGRSYSELIALGTASFVAFGLFSLPAGWLADRWSRRNMMVAFYVGCGVS
                     LLGAAVAPSLWGLAIALFALGLFAAIYHPVGTAIIIESATVRGRTLAFNGVCGNLGVS
                     LAAGVTAVLTAALSWRGAFLVPGLICVATGAAYLWLVPNEKGHAAARSNAADVALSPA
                     AAAVIFALFAVIALSAGLVFNTLAVALPKIVDERVGSDISLVAVGGLTTAIFMCGAIA
                     QISVGRLVERFPVHLLFGVTAILQFAGIVWAAYANGVMLLVALAFTFAAIYGQITVND
                     LVIARYTADAWRGRVYAVRYFLTFMVSGAAVSLIAFLYARGGFGLVLGANALLALGFL
                     IAVLLVVVIANGVERSRVPQAAPAE"
     gene            complement(2071..2304)
                     /locus_tag="VGP50_01545"
     CDS             complement(2071..2304)
                     /locus_tag="VGP50_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026079.1"
                     /translation="MTKYLGSEISSDEAVEMMLLRLAALAIDLRERLTPEEIAKLPSL
                     WVVMEACNDLLTDLGWGPLAEPLRQNRPPSLMQ"
     gene            complement(2375..3034)
                     /locus_tag="VGP50_01550"
     CDS             complement(2375..3034)
                     /locus_tag="VGP50_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="HEV8026080.1"
                     /translation="MTQVHVVRHDNSQLYATQLDQYFRRRYEVFVRERGWRDIERPDG
                     RDIDQFDTEDAIHLLAIDKDEVVGGLRFNPSSGPTLLNDVYPHLCFGPLSRSPDIHET
                     TRLWVVKERRSEASLPSVEALLVAASLELSIALGIRKTRVLFETRRLGRNKRLGWTLG
                     PLGPPHEIDGADFIAVEKEVSEAIWAGICRQLSVPGPVLVWNGTARPSYRLRELAPAM
                     A"
     gene            complement(3171..3863)
                     /locus_tag="VGP50_01555"
     CDS             complement(3171..3863)
                     /locus_tag="VGP50_01555"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEV8026081.1"
                     /translation="MEFVTALEAATGCSDVMQLFGREIAALGFNSYIMVALDERTLTQ
                     RLMASGWHPEWIATYTAENLSAADPVRRQASRAINPFFWSEVSYNPEREPRAKEIMEL
                     AADFQMNEGFCLPIRDGNTIAAISIAGDKPDLGHGVKAALHIMSLFAYNRFRALTNPP
                     LQSSLLTEREREALRWVSIGKSDWDISAILHVSERTARAHVLSAVHKLKAANRSAAVA
                     EALSRGEIPLNY"
     gene            complement(4086..>4680)
                     /locus_tag="VGP50_01560"
     CDS             complement(4086..>4680)
                     /locus_tag="VGP50_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV8026082.1"
                     /translation="AALVLAAGRSSRMGGANKLLAEIGGRPLVRIAVEEALASQARPV
                     IVVTGHQRERVEAALAGLPVDFVHNPYFADGLGTSLKTGIAALPAQSDGVIVCLGDMP
                     QVDAAMIDRLIGAIDPAKGALIAMPTIEGQRGNPVVWSRRFFPDLMAVEGDVGARYLI
                     GRYTEAVAEVPLSGKAALTDVDTPEVFEAVKAELEGT"
BASE COUNT          836 a         1525 c         1597 g          722 t
ORIGIN      
        1 tcttctccgg cctgcccggc accgcgctgt gggcgctcgg gctcctggtc gggatcaatc
       61 tcttgttcgg cggcagcgcg ctcatcgcca tggcgctgtc ggcacggcag accgcctgaa
      121 ccacgacgct cagcggacga cgtagtggcg atgctggtgg cgcgattggc gctggcgacg
      181 gcagcggcct tcgccggcgc cgccctctat gtcaacgtcg ccgagcaacc cgcccggctc
      241 ggtctcgacg accgcgccct gctggcgcag tggaaaccaa gctacagccg gggcgccgcc
      301 atgcaggcga gcctcgccgc gatctccggc ctgctcgggc ttgctgcttt ctggttgagc
      361 ggggattggc gctggctcat cggggcggcg ctcatcctcg ctaattggcc gtacacgctc
      421 ctctgcatca tgccgaccaa caaccggctc aaggcgatcg ccgacgatgc ggcaggcgcg
      481 gcgtcacggg cgctgatcga gagatgggga tggctgcacg cggcgaggac cgcgctcggc
      541 atcgccgcga ccgcggccta tctgtgggcg ctgagctgaa cggcgggcgt ttggcgggca
      601 aattcactcc gccggcgccg cctgcggcac ccgcgagcgc tcgacgccat tggcgatgac
      661 cacgaccaac aggaccgcga tcaggaagcc gagcgccagc agcgcgttgg cgccgagcac
      721 gaggccgaag ccgccgcgcg cgtagaggaa cgcgatcagc gacaccgccg cgccggaaac
      781 catgaaggtc aggaaatagc gcaccgcata gacacggccg cgccaggcat cggcggtgta
      841 gcgcgcgatg accaggtcgt tgaccgtgat ctgcccgtag atcgccgcga aggtgaaagc
      901 gagcgcgacc agcagcatga cgccgttcgc atacgccgcc cagacgatgc cggcgaactg
      961 caaaatcgcg gtcacaccga acaggagatg caccgggaag cgctccacca agcggccgac
     1021 gctgatttgc gcaatcgccc cgcacatgaa gatcgcggtg gtgagcccgc cgacggcgac
     1081 gagcgagatg tcgctgccga cgcgttcgtc gacgatcttc ggcaaggcca cggcgagcgt
     1141 gttgaagacg aggccggcgg agagcgcgat caccgcgaac agcgcgaaga tcacggcggc
     1201 ggccgccggc gacagcgcca cgtcggcggc gttgctgcgc gcggcagcgt gccccttctc
     1261 gttcgggacg agccaaagat aggccgcccc ggtggcgacg cagatcaggc cgggcacgag
     1321 aaaagcgccg cgccaggaga gcgccgcggt gagcaccgcg gtcacccccg ccgccagcga
     1381 aacgccgaga ttgccgcaca cgccgttgaa cgccagcgtg cggccgcgca ccgtcgcact
     1441 ctcgatgatg atcgcggtgc cgaccggatg atagatcgcc gcgaacaggc cgagcgcgaa
     1501 cagcgcgatc gccaggcccc acagcgaggg cgctaccgcc gcgccgagaa gcgacacgcc
     1561 gcagccgaca tagaaggcga ccatcatgtt gcgccggctc cagcggtcgg cgagccagcc
     1621 ggccggcagc gagaacaggc cgaaggcgac gaaggaggcg gtaccgagcg cgatcagctc
     1681 ggaataggaa cggccataga ccgcttcaag ctcgatgacc acggtcggaa agatcagcat
     1741 cacatagtga tcgagcgcat gcgcccaatt gataaagccg atcgccacgc gccggcgcga
     1801 gagctcggcg gtgcgggcgg cggggaatcg ggcggaggtc gaaacttcgg cggtcatggg
     1861 gtcccggatt ggtcgattgc attgcatccc gctgttgcgg aaaggcgcgg gagatatatc
     1921 gcaccgtgct gcctcgacct acccctattc tgcagggcag ctcggcgtgg cgccggtgcg
     1981 gagggtcaac cgggaatgcc gggaggggcg gtcgggaata tagagagacg ccgggaacgg
     2041 ccgcgccgca gcgtcactac aatggcgcca tcactgcatg aggctgggcg gtcgattctg
     2101 acgcaacggc tccgccagcg gtccccagcc gaggtccgtc aacaaatcgt tgcaggcttc
     2161 catgacgacc cagagactgg ggagcttcgc gatctcttcc ggcgtcagcc gctcgcgcag
     2221 gtcgatggcg agcgccgcaa gacgaagcag catcatctcg accgcttcgt cggaagagat
     2281 ctccgacccg agatatttgg tcatggtcac gctccgctat ggttgggatt ctcgccgcgc
     2341 cggcgagccg ccgagggttg atttgattgc cgtctcacgc cattgccggg gccaattcgc
     2401 gaagccggta ggagggccgc gccgtgccgt tccagaccag gaccgggccg gggacggata
     2461 gctgccggca gatgccggcc cagatcgcct cggagacctc tttttcgacg gcgatgaaat
     2521 cggcgccgtc gatctcgtgg ggagggccca gcggccccag cgtccagccg agtcttttat
     2581 tccggcccag tcgccgcgtt tcgaagagca cacgggtttt tcgtatcccg agcgcgatcg
     2641 agagctcgag cgaggccgcg accaacaaag cttcgacgct gggcagcgaa gcttcactgc
     2701 gccgctcctt gaccacccac aaccgggtgg tctcgtggat gtccggcgag cggctgagcg
     2761 ggccgaagca aagatgcgga tagacgtcgt ttagtaaggt cggcccgctc gagggattga
     2821 agcgaagccc gccgacgacc tcgtccttgt cgatggcgag gagatgaatg gcgtcctcgg
     2881 tgtcgaattg atcgatgtcg cggccgtccg ggcgttcgat gtccctccag ccgcgttcac
     2941 ggacgaagac ctcgtaacgg cgtctgaaat attggtcgag ctgcgtggca tagagttgcg
     3001 aattgtcatg tcgcacgaca tgcacttgcg tcatcgattc tcccgcggcc tgagcgcgcg
     3061 gggaacgatg ggcggagcct tactcgcgga cgtattgtcg aaaccggcaa ttcaaacagg
     3121 cgcgcgcggg cgtcgcgggc gcacgcggtc gacagagtaa tttaatattt tcagtagttt
     3181 agcgggatct cgccgcggct gagcgcttcg gcgacggcgg cggagcgatt cgctgcctta
     3241 agtttgtgca cggcgctgag cacatgcgcg cgcgccgtcc gctcgctcac atgcaggatg
     3301 gcgctgatgt cccaatccga cttgccgatc gagacccagc gcaacgcctc gcgttcgcgc
     3361 tcggtcagca ggctcgactg caagggcgga ttggtcagcg cgcggaaccg gttgtaggcg
     3421 aacagcgaca tgatgtgcag cgccgccttg acgccgtgac cgaggtccgg cttgtcgccg
     3481 gcgatgctga tcgccgcgat cgtgttgcca tcgcggatcg gcaggcaaaa gccttcgttc
     3541 atctgaaaat cggcggcaag ctccatgatc tccttggcgc gcggctcgcg ttcgggattg
     3601 taggagacct cgctccagaa gaacgggttg atcgcgcgcg aagcctgccg gcgcaccgga
     3661 tcggcggcgc tgagattctc cgccgtgtag gtcgcgatcc attccggatg ccagccgctc
     3721 gccatcaggc gctgcgtcag cgtgcgttcg tccaacgcga ccatgatgta cgagttgaat
     3781 ccgagcgcgg cgatttcgcg gccaaagagc tgcatcacgt cgctgcagcc ggtcgcggct
     3841 tcaagcgcgg tgacgaactc caacgtcctg acgccccaaa ggggatcagc tggcgctatt
     3901 cctcccacag atttggccct tggcggttat ccacgccccc ggggccggag ggtatttaca
     3961 caccatcggc gcgcaagaca ttcgttggca cgctgtggaa aagtcgcggc cttttggtcg
     4021 ccagcgaagg cgcggaggcg tttccccttg gcgaatagtc accgcgacgg cggcgagcga
     4081 tcttattacg tcccctcgag ctccgccttc accgcctcga acacctccgg cgtatcgacg
     4141 tcggtgagcg cggctttgcc ggagagagga acttcggcga cggcctcggt gtagcgcccg
     4201 atcaggtagc gcgcgccgac atcgccttcc accgccatga ggtccgggaa gaaacgccgc
     4261 gaccagacca ccggattgcc gcgctggcct tcgatcgtcg gcatcgcgat cagcgcgccc
     4321 ttggccggat cgatggcgcc gatcaggcga tcgatcatcg cggcatcgac ctgcggcatg
     4381 tcgccgaggc agacgatgac gccgtccgat tgcgccggaa gcgccgcaat gccggtcttg
     4441 agcgaggtgc cgaggccgtc ggcaaaatac ggattgtgca cgaaatcgac cggcaggccg
     4501 gcgagcgccg cctcgacgcg ctcgcgctga tggccggtga ccacgatcac cggccgcgcc
     4561 tgcgaggcga gcgcctcctc gacggcgatg cgaacgagcg gccggccgcc gatctcggcc
     4621 aggagcttgt tggcgccgcc catgcggctc gagcggccgg cggcgagcac gagcgccgca
//
LOCUS       DASYNS010000051         5671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1730550, whole genome shotgun sequence.
ACCESSION   DASYNS010000051 DASYNS010000000
VERSION     DASYNS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730550"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1299
                     /locus_tag="VGP50_01565"
     CDS             226..1299
                     /locus_tag="VGP50_01565"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF009005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEV8026083.1"
                     /translation="MKPGLEGVIAAETVLTHVDGARGIWWLRGHSLGELVADHGFEGA
                     VALLWDGFAGEGLTRDDILATLGDGRIAAFERLPHWLAAAGHRPLSEAMRLLFAAIPE
                     GSTPGAIAAAVPVGLAALIRGRAGEPPVAPDPALTTAADFLRMAAGAPASAASAAALD
                     AYLTTVVDNGLAASTFAARVIASTRASLAAAVLGGYCALTGPLHGGAPGPVLDMLDEI
                     GSVDKIDAWLEQALARADRLMGFGHRVWKRRDPRADALKATLRRLGPDAGRIAFAEEV
                     ERRAIAALHRLKPDRVLETNVDFYTSLLLEAIGLPRDAFTPVFALARTPGWLAHALEQ
                     QKTGRLIRPAAAYIGPHPHPRAA"
     gene            1343..1960
                     /locus_tag="VGP50_01570"
     CDS             1343..1960
                     /locus_tag="VGP50_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEV8026084.1"
                     /translation="MNDRPDQRRYAPAVARNRAPILAVLRERLPASGLVLEVASGSGE
                     HAAFFAGELPHLTWQPSDTDPRALDSIAAFRAAAGAPNLLPPLRLDAEAETWPVRAAD
                     AILSINMIHIAPWSAATGLLAGAARLLPPGGLLYLYGPFMEQGRHTAPSNAAFDADLR
                     ARDPRWGVRDLDIVAELARRHGLALAETVAMPANNRSVLFRRAER"
     gene            1967..2728
                     /locus_tag="VGP50_01575"
     CDS             1967..2728
                     /locus_tag="VGP50_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEV8026085.1"
                     /translation="MGGLGFLLRGYGLARSAVIYHGNPLKRRRARAFYRQFVRPGDLC
                     FDIGAHLGDRTGHFLALGARVVAVEPQPGALAVLRHLHGRDRRVTIIAAAAGAAPGEA
                     ELSVDPANPTVASLSPEWRAQVSRSPRFSGIRWRESRRVPVTTLDAIIAEHGLPDFCK
                     IDVEGFEAEVLMGLTQPIPALSFEYIPEVVGAAETALERLAALGVYRFNRSVGESMRF
                     VAPRWQGADEMARDLAGLAADAGSGDIYARLVAAL"
     gene            complement(2744..3085)
                     /locus_tag="VGP50_01580"
     CDS             complement(2744..3085)
                     /locus_tag="VGP50_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026086.1"
                     /translation="MAEDSLMTALAHWRWLAALPLFVATCGDAPVPGATSADPALQHD
                     VTNLLTMMDSGRAKGCSQRKVVDTQSIETAPDHQTERWVIDRCGERVGYSVTFKPNSH
                     GGTDFNIQPEK"
     gene            3205..4368
                     /locus_tag="VGP50_01585"
     CDS             3205..4368
                     /locus_tag="VGP50_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026087.1"
                     /translation="MASRGKIVVLHFVGQMPLAGIAWQAMHYVLGLERLGYEAWYVED
                     HGANPYDPRVNSVVMDCAYNVAYVRRMMESYGLGDRWAYWDAINDVYHGLSREAVHRL
                     YREADALINLCGAVKLRDEHMACPRRIMIDTDPVYEQIKYAAADPVARAYIDAHTHFF
                     TYGENVGGPGWVVPLCGIPWRGTRPPVVIEEWPTDAESPDCFSTIATWENKGKNIAFG
                     GETYLWSKHMNFLRFLDLPKRLKHCFIMAMLPPTPEVETQVTGAGWRLTDPRPISASM
                     ESYAAFIAGSRGEFTVAKDIYVRPNSGWFSDRSVCYLAAGRPVVTMRTGFSRFYPVGD
                     GLFEYSDSEEAAAAIETINGDYRHHSRAARMMAAEYFNSDSVISELMAEVGFS"
     gene            4388..>5671
                     /locus_tag="VGP50_01590"
     CDS             4388..>5671
                     /locus_tag="VGP50_01590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV8026088.1"
                     /translation="MSGENRRAAAAMRSPFGSVAPTSRNFWDSAAFALFLGALVLVIA
                     TFTDYGVTWDEDVHHWYGVFVLKYYLSGFTNLRALDWGDLYYYGAAFDTTAAAIAQIS
                     PFGTYETRHLLNGVVGVIGLIGVWKLGRALAGPRAGFVAALFLLLTPNYYGQMFNNPK
                     DIPFAVGMTWATYYLVRIIPELPRPRWSLLVKLGLAAGLALGVRVGGLLLLGYLGLLL
                     LLFVLWRGLETRRLRGFVAESWTCFWRVLLPVTLIAWPVMLIFWPWAQQSPVLNPLRA
                     IAYFSHEIFPFRTLFAGQYVLASDLPWAYLPVHIALALPELLLVLLLAAPVTAVVMAR
                     RRGWRLERDHALGFFIVGFGIVFPVAYAIAIKAVLFDGMRHFIFVLPLIAALTAVLAD
                     RALDVMARLPQRRYAYGLLGIYALSHVSVMARLHPD"
BASE COUNT          771 a         2021 c         1835 g         1044 t
ORIGIN      
        1 agcgccgcgc cctgccgccg gtcgcctcgg agcggaccag ccctcggctc acataggcat
       61 agagcgtggc cgggcgcacg ccgagagccg ccgccgcctc cctggcgctc agatagtgac
      121 gcccgcttct ggccattccc acctccaata tgttgattgt atgatcaata ttgaccgtat
      181 gtatagccgg atcgatatta gcagtggttc gttcaggagg cagccatgaa accagggctt
      241 gaaggcgtga tcgcggcgga gacggtcctg acccatgtcg atggcgcgcg cggcatctgg
      301 tggctgcgcg gccactcgct cggcgagctc gttgccgatc atggtttcga gggcgcggtg
      361 gccctgctgt gggacggctt tgccggcgag gggctcacgc gtgacgacat ccttgccacc
      421 cttggcgatg gccgcatcgc cgccttcgag cggctgccgc attggctggc ggccgccggc
      481 catcggccgc tgagcgaggc catgcgattg ctgttcgccg ccattcccga gggcagcacg
      541 cccggggcca ttgccgccgc ggttcccgtc ggcctcgcgg cgctgattcg cggccgcgcc
      601 ggcgagccgc cggtcgctcc cgatccggcg ctcaccaccg ccgccgactt cttgcggatg
      661 gcagcgggtg cgccggcgtc ggcggcttcc gctgccgctc tcgacgccta tctcaccaca
      721 gtcgtcgaca acggcctcgc cgcctcgacc tttgccgcgc gggtcatcgc ctcgacgcgc
      781 gcctcgctcg cggccgcggt gctcggcggc tattgcgcgc tcacgggccc gctgcatggc
      841 ggcgcgcccg gccccgtgct cgacatgctc gacgagatcg gcagcgtcga taagatcgac
      901 gcctggctcg agcaggcatt ggcccgcgcg gacagactca tgggctttgg ccatcgcgtc
      961 tggaaacggc gcgacccccg cgccgacgct ctgaaggcga cgctgcgccg cctcggtccc
     1021 gatgccggac gcatcgcctt tgccgaagaa gtcgagcggc gcgccatcgc cgcgctccat
     1081 cgcttgaagc ccgaccgcgt tctcgagacc aacgtcgact tctacacgtc gctgctgctc
     1141 gaggccatcg gcctgccgcg ggacgccttc acaccggtct tcgcgctcgc ccgcacgccc
     1201 ggttggctcg cccacgcttt ggagcagcag aagaccggcc gtctcatccg ccccgccgcc
     1261 gcctatatcg gcccccaccc gcatccgcgc gcggcctgaa atccctctct ccgaggagcg
     1321 gaagaggggc tagagttgcg ccatgaacga tcggcccgat cagcgccgtt atgcgccggc
     1381 ggtggcgcgc aatcgtgcgc ccatccttgc cgtgctgcgc gagcggctgc ccgcctcggg
     1441 gctcgtgctc gaagtagcga gcggcagcgg cgagcacgcg gctttcttcg cgggcgagct
     1501 gccgcatctg acgtggcaac cgagcgacac cgatccccgc gcgctcgaca gcatcgccgc
     1561 cttccgcgcc gcggccggcg ctcccaacct gttgccgccg ctgcgcctcg acgccgaagc
     1621 ggagacatgg ccggtgcggg cggctgacgc gatcctctcc atcaacatga tccacattgc
     1681 gccgtggtcc gccgcgacgg gcctcctggc gggtgcggcg cggctcttgc cgccgggcgg
     1741 tctcctctac ctctacggtc ccttcatgga gcaaggccgt cacacggcgc cgagcaacgc
     1801 cgccttcgac gccgatcttc gcgcgcgcga tccgcgctgg ggcgtgcgcg acctcgatat
     1861 cgtggccgag cttgcccggc gccacggcct cgcgctcgcc gagaccgtcg ccatgccggc
     1921 gaacaatcgg agcgtgctct tccgccgcgc ggagcgctga gccgggatgg gcgggctggg
     1981 cttcctcctc cgcggctacg ggctggcgcg ctctgccgtg atctatcacg gcaatccgct
     2041 gaagcgccgg cgcgcccgcg ccttttatcg gcaattcgtg cgaccgggcg atctctgctt
     2101 cgacatcggc gcgcatctcg gcgaccgcac cgggcatttc ctggcgctgg gcgcgcgcgt
     2161 cgtcgccgtc gagccgcagc ccggagcgct cgccgtgctg cgccatctcc atggccgcga
     2221 ccggcgcgtc accatcattg ccgcggcggc cggcgcggca ccgggcgagg ccgagctcag
     2281 cgtcgatccg gcgaacccca cggtcgcttc gctctcgccc gaatggcgtg cgcaagtatc
     2341 gcgctcaccg cgtttcagcg gcatccgctg gcgtgagagc cgccgggtcc ctgtcaccac
     2401 gctcgacgcc atcatcgccg agcacgggct tcccgatttc tgcaagatcg atgtcgaggg
     2461 cttcgaggca gaggtgctga tgggccttac gcagccgatc ccggcgctct ccttcgaata
     2521 cattcccgag gtcgtcggtg ccgccgaaac agcgctcgag cgcctcgccg cgctcggcgt
     2581 ctaccgcttc aatcgctctg tcggcgagag catgcgcttc gtcgcgcccc gatggcaggg
     2641 cgccgacgag atggcgagag acctcgccgg cctcgcagcc gacgcgggat caggcgacat
     2701 ctacgcgcgg ctcgttgccg cgctctgatc cggcgcggcg cgcttatttc tccggctgta
     2761 tattgaagtc ggtgccgccg tggctgttcg gcttgaaggt gacgctgtag ccgacgcgct
     2821 cgccgcagcg atcgatgacc cagcgctcgg tctggtggtc cggcgccgtc tcgatgctct
     2881 gcgtatcgac gacctttcgc tggctgcatc ctttggcgcg gccggaatcc atcatcgtca
     2941 ggagattggt cacgtcatgc tgcaatgccg ggtctgccga ggtcgcgcct gggaccggcg
     3001 catcgccgca ggtcgcgacg aagagcggca gagccgcgag ccatcgccaa tgtgcaagcg
     3061 ccgtcatcag ggagtcctcc gccatccgca ccctcgcgcc ggggcgcccg gcggtcaatt
     3121 agggcattcc cgccccggca acgccataga atcttcagac ttttgcggct aagtttccgg
     3181 actttttttc gcggagagcc gggcgtggcg tcgcgtggca agatcgtcgt cctgcatttc
     3241 gtcgggcaga tgcccttggc gggcatcgcc tggcaggcca tgcactacgt cctcggcctc
     3301 gagcgcctgg gctacgaggc ctggtatgtc gaggaccacg gcgccaaccc ctatgacccg
     3361 cgcgtcaaca gcgtggtgat ggactgcgcc tacaatgtcg cctatgtgcg ccgcatgatg
     3421 gagagctatg ggctgggcga ccgctgggcc tattgggacg cgatcaatga cgtctatcac
     3481 gggctctcgc gcgaggccgt gcatcgcctt tatcgcgagg ccgatgcgct catcaatctc
     3541 tgcggcgcgg tgaagctgcg tgacgagcac atggcttgcc cgcggcgcat catgatcgac
     3601 accgacccgg tctatgagca gatcaaatac gctgcggccg atcccgtggc gcgcgcctat
     3661 atcgacgcgc atacgcattt cttcacctat ggggagaatg tcggcggccc cgggtgggtc
     3721 gtgccgctct gcggcattcc ctggcgcggc acgcggccgc ccgtcgtcat cgaggaatgg
     3781 ccgaccgacg cggagtcgcc cgactgcttc tcgaccatcg ccacctggga gaacaagggc
     3841 aagaacatcg ctttcggcgg cgagacctat ctctggtcga agcacatgaa cttcctgcgc
     3901 ttcctcgacc tgccgaagcg cctcaagcat tgcttcatca tggccatgct gccgccgacg
     3961 cccgaggtcg agacgcaggt gaccggcgcc ggctggcgtc tcaccgaccc gcggccgatc
     4021 tcggcgagca tggagagcta cgccgccttc atcgccggat cgcgcggcga gttcaccgtc
     4081 gccaaggaca tctatgtgcg ccccaacagc ggctggttca gcgaccgcag cgtctgctac
     4141 ctcgcggcgg ggcgtccggt ggtcacgatg aggacgggct tcagccgatt ttacccggtc
     4201 ggcgacgggc ttttcgaata cagcgatagt gaagaggcgg cggcggcgat cgagaccatc
     4261 aatggcgact accgccatca cagccgcgcc gcgcggatga tggccgcgga atatttcaac
     4321 agcgacagcg ttatttccga attgatggcg gaggtcggtt tttcctagcg atgatgggaa
     4381 catcaccttg agcggagaga accggcgggc ggcggcggcg atgcgctcgc cgtttggctc
     4441 ggttgcgccg acctcgcgca acttctggga tagcgcggca ttcgcgcttt tcctcggcgc
     4501 gctcgtgctg gtgatcgcca ccttcaccga ctacggcgtc acctgggacg aggacgtcca
     4561 tcactggtat ggcgtcttcg tcctcaaata ttacttgtcg ggcttcacga atctccgcgc
     4621 cctcgactgg ggcgatcttt attactacgg cgccgccttc gacacgacgg cggcggcgat
     4681 cgcgcagatc tcgcccttcg gcacctacga gacgcgccat ctgctgaacg gcgtcgtcgg
     4741 cgtcatcggc ctgatcggcg tgtggaagct gggccgcgcg ctcgccggtc cccgcgccgg
     4801 ctttgtggcc gcgctcttcc tcctgctcac gcccaattac tacgggcaga tgttcaacaa
     4861 ccccaaggac atcccgttcg ccgtgggcat gacctgggcg acctattacc tcgtgcgcat
     4921 catccccgag ctgccgcgcc cgcgctggtc cttgctcgtc aagctcggtc ttgccgccgg
     4981 gctcgcgctc ggcgtgcgcg tcggcggtct cctgctgctg ggatatctcg gactgctgct
     5041 cctgctcttc gtgctgtggc gcggcctcga gacgcggcgc ctccgcggct tcgtcgcgga
     5101 gtcctggacg tgcttctggc gcgtactgct gccggtcacg ctcatcgcct ggccggtgat
     5161 gctgatcttc tggccctggg cgcagcagtc gccagtcctc aacccgctgc gcgccatcgc
     5221 ctatttctcg cacgagatct tcccgttccg tacgctcttc gccgggcaat atgtcctggc
     5281 aagcgacctg ccctgggcgt atctgccggt tcacatcgcg ctcgctttgc ccgagctgct
     5341 gctcgttctg cttttggccg cgccggtcac ggccgtggtc atggcgcggc gccggggctg
     5401 gcgcctggag cgcgaccacg cgctcgggtt cttcatcgtc ggcttcggta tcgtttttcc
     5461 cgtcgcttat gccatcgcca tcaaggccgt gctgttcgac ggcatgcgcc atttcatctt
     5521 cgtgctgccg ctcatcgccg cgctcaccgc cgtgctggcc gatcgcgcgc tcgacgtcat
     5581 ggcgcggctg ccgcagcggc gctatgccta tggcctgctc ggcatctacg ccttgagcca
     5641 cgtctcggtg atggcgcggc tccatcccga c
//
LOCUS       DASYNS010000052        13377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1730843, whole genome shotgun sequence.
ACCESSION   DASYNS010000052 DASYNS010000000
VERSION     DASYNS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 13377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13377
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730843"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VGP50_01595"
     CDS             <1..1149
                     /locus_tag="VGP50_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8026089.1"
                     /translation="GVGAARVRDLFEQARLKAPAIIFIDELDALGRARGAYPGMGGHD
                     EKEQTLNQLLVELDGFDSRSGLVLLAATNRPEILDPALLRAGRFDRQVLVDRPDKAGR
                     IDILKVHLKGHTLAADVDPEKIAALTPGFTGADLANLVNEAALIATRHKADTVTMDDF
                     TNAVERMVAGLEKRKRVLNAKERRVVAHHEMGHTLVAMSLPGTDTVHKVSIIPRGIGA
                     LGYTIQRPSEDRFIMTREDLEDRMAVLLGGRAAEHIVFQHFSTGAADDLAKVTSIARD
                     MVARYGMEEKLGPVSYDAQRPTFLQGAPPPEGWHERSYSDETAHAIDQAVQQIVERAF
                     ARTVALLERRRDLLERGAKLLLEKETLDEADLKALQAELTAKDAVAAQ"
     gene            complement(1198..1950)
                     /locus_tag="VGP50_01600"
     CDS             complement(1198..1950)
                     /locus_tag="VGP50_01600"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEV8026090.1"
                     /translation="MRIGAITVIALGLLTAACGTNTQQRAATGGLTGLGVGALVGGPV
                     GAAVGVLVGAAGGWAMPEGADELAFNAIGAEHNVASGALNEAGLGPSSPGPGQASGLV
                     RDAQLKLQRDRFYNGPIDGIVGPETERALSAYQRRHGLQETASLDEATLRRMNLAGGT
                     AASGSSTPPAAMRSDQIRDTLLSDGYDNISQLHRQPNDTYTAQADRNNATYRLRIDAH
                     TGRVITQRQLASNPATPAGGSTSTDTSGSDNH"
     gene            2083..2250
                     /gene="rpmG"
                     /locus_tag="VGP50_01605"
     CDS             2083..2250
                     /gene="rpmG"
                     /locus_tag="VGP50_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV8026091.1"
                     /translation="MAKSNTILIKLVSSADTGYFYVTKKNPRAKTEKMELRKYDPIAR
                     KHVVFKESKIK"
     gene            complement(2262..3659)
                     /locus_tag="VGP50_01610"
     CDS             complement(2262..3659)
                     /locus_tag="VGP50_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HEV8026092.1"
                     /translation="MSARVLVVDDVDVNVRLLEAKLSSEYFTVLTASSGEQALQAVKS
                     ERPDIVLLDVMMPEMDGFEVCRRLKSDPKTADIPVIMVTALSEVSDRVRGLQAGADDF
                     LTKPVNDVALFARVRSLVRLKRMTEEWRLREEVYGRFETGPREARPSEDMSPARIVLW
                     EENRFAGDRLTEMLAPVAAEVLRPSSPDELRALMDPAVDLVILSLAGQQDALRLLAQL
                     RTADPHRFVPILLVADGEEVPRLAKGLDLGANDYIVRPIDRNELTARARTQIRRKRLQ
                     DGLVDNYQRSLALALTDGLTGLYNRRYLMAHLDGLMARAADGQNGTALLMIDIDYFKR
                     VNDTHGHAAGDAVLREIAGRIQRHVRSFDLVARYGGEEFVVVMPDTPLSVAALVAERL
                     RNTIADKPVGLGEGASEKQVTVSIGIAMTRQKGDTGGALLQRADKALYSAKGAGRDCV
                     FSFPADALTRSSAIS"
     gene            complement(3674..4051)
                     /locus_tag="VGP50_01615"
     CDS             complement(3674..4051)
                     /locus_tag="VGP50_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8026093.1"
                     /translation="MTKRSSKRVLIVEDNDLNMKLFHDLLEAHGYATLQTKDGMEALK
                     LARQHHPDLILMDIQLPEVSGLEVTKWIKEDDDLRSIPIIAVTAFAMKGDEEKIREGG
                     CEAYIAKPISVANFLQTVERFLS"
     gene            4167..5075
                     /locus_tag="VGP50_01620"
     CDS             4167..5075
                     /locus_tag="VGP50_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV8026094.1"
                     /translation="MRIGVDLGGTKIELAALDRDGAILLRRRIATPGNDYAAIIRAVT
                     ALVDAAERTLGRRGTVGIGMPGALSPATGLVKNANSTCLIGKPFDRDLISALGRPVRF
                     ANDANCFALSEAVDGAAAGAAIVFGTILGTGVGAGIVVDGRVLTGANAIAGEWGHSPL
                     PWPEDEERPGPRCYCGKHGCIESFLSGPGLALDHRSATGEALEATAIAAAAGAGDPEA
                     EATLARYEDRLARALATVINLLDPDVIVLGGGLSAIARLYQTVPRRWGAYVFSDAVAT
                     RLAPPRHGDSSGVRGAAWLWAPDEAP"
     gene            5072..5260
                     /locus_tag="VGP50_01625"
     CDS             5072..5260
                     /locus_tag="VGP50_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026095.1"
                     /translation="MTTLCRDCASLAPAPAGGRCAACGSPRLMSHPEIETLSLAHIDC
                     DAFYAGVEKHLGRDDLHP"
     gene            5214..5519
                     /locus_tag="VGP50_01630"
     CDS             5214..5519
                     /locus_tag="VGP50_01630"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026096.1"
                     /translation="MPASRSISAETTFTHDIAAYEALAEELRPLCDSVAHCLQRANKA
                     ATGVTLKLKTSDFQVPSRSRKLADPTRRAEILYRRPARRSGAGGSARPVRRAHRMSM"
     gene            5684..7678
                     /locus_tag="VGP50_01635"
     CDS             5684..7678
                     /locus_tag="VGP50_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8026097.1"
                     /translation="MSRRRERKGRPKRPRGSMRKAVAEARQANRFLDAIIENIPHMVF
                     VKDARDLRFIRSNRAGETLLGVARTDLVGKSDYDFFPESDAAFFQAKDRDTLKGGTLS
                     DIPEEAIDTRTGRRWLHTKKVPILDSTGAPKYLLGISEDITEHKRMEEARSRLAAIVD
                     SSDDAIFGKSFDGVIATWNGAAERLFGFTADEIVGKLMWILIPERHRGEEAAIFERLK
                     TGERIEPYETARTRRDGSEVEVWVTLSLIKDQSAKTIGISTIARDFTEMKRTRRALVE
                     ANDAAEAAAQALQQAQKLQALGRLAAGVAHHFNNLLTVVIGNLEEGTARQSAAGLARE
                     PMLTAALSAALRGSGLTRQLLSFSRQQLLQPEIIEPSKRLADTAFLFAASLRKDITIE
                     TDIPDHLWPIVVDPAELELALLNLALNARDAMPAGGVLRLTAVNQTIEGARPGLAGPY
                     LVIGLSDNGTGISPEILPRVFDPFFTTKDVGSGSGLGLSQVHGFAHQSGGTVGIETTP
                     GKGTTVRLYLPASPGARQAAPAAEASAYCRAGAGTVLVVEDDADVAALAAESLRHRGF
                     RVKLADRAQAALDLLSQGETVDLIFSDIVMPGGMNGLELAEAVRARFPTIPVLLTTGH
                     SDAVTDAMTRGLQIIAKPYRLAEICDRVARLIRATTVAGA"
     gene            complement(7685..8500)
                     /locus_tag="VGP50_01640"
     CDS             complement(7685..8500)
                     /locus_tag="VGP50_01640"
                     /inference="COORDINATES: protein motif:HMM:NF020478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HEV8026098.1"
                     /translation="MISWSLRLIGVAAGFCVAALPASAAEIAPHRALYSLTLGSAKQS
                     SGVVAATGAMAYEWGETCDGWTVQQRFRLRLQYAEQDGVEVSSSLVTWESKDGLRYRF
                     NERRLRNGEPDEEIKGEARLDGAGKGGTAEFTRPEASTLTLAPGVLFPTAHTLLLIDR
                     AQAGEQFVAKAVFDGASVENASQITAVIGPELKPDAPKAGEKPVDSPLLNRPSWRIRL
                     AFFPPDTKVEQPDYELGMRLLDNGVSQDMALDYSDYVIRAKLDEIEALPRPNC"
     gene            complement(8610..10403)
                     /gene="aspS"
                     /locus_tag="VGP50_01645"
     CDS             complement(8610..10403)
                     /gene="aspS"
                     /locus_tag="VGP50_01645"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV8026099.1"
                     /translation="MHAYRTHSCGALRLGDAGRVARLSGWVHRKRDHGQLLFIDLRDH
                     HGITQCVIDSASPLFPLADAQRLESVITVTGTVVKRSPETVNPKLPTGEVELQIAELQ
                     VLSEAEPLPFPVNADADYPEEMRLRYRFLDLRRENMHANIVLRSNVIASLRRRMIEQG
                     FAEFQTPILTASSPEGARDYLVPSRLHPGKFYALPQAPQQFKQLLMVAGFDRYFQIAP
                     CFRDEASRADRSPGEFYQLDFEMSFVTQEDVFAAIEPVLAGVFAEFGGGKPVTAPPFP
                     RLTYEDALLRFGSDKPDLRNPLLISEVRDVFRGSGFKVFAGAVAEGAVVRAIAVPGVA
                     AQPRSFFDKLNDWARGEGAPGLGYIIFAEGAAKGPIAKFLDEPRLQQLKTLAGLKDGD
                     AVFFVCDKKAAAEKLAGAARTKIAQELNLIAKNDFRFCWIVDFPMYEWNEEEKKIDFS
                     HNPFSMPQGGLEALLTKDPLEINAFQYDIVCNGVELSSGAIRNHRPDIMYKAFEIAGY
                     AAAEVEARFGGMLNAFKYGAPPHGGSAPGIDRIVMLLAETPNIREVVAFPMTQQAQDL
                     LMQAPAAVPPERLKELHIRLALPPETKSKSS"
     gene            10562..11734
                     /gene="rnd"
                     /locus_tag="VGP50_01650"
     CDS             10562..11734
                     /gene="rnd"
                     /locus_tag="VGP50_01650"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEV8026100.1"
                     /translation="MTLIDNTEALAAFCRRQAGAGYVTVDTEFMRDKTYWPQLCLVQI
                     AGPDEAAAIDPLAPGIDLSPLFELMADSRLLKVFHAARQDIEIFVNLTGAVPAPLFDT
                     QVAAMVCGFGDAVSYETLASRLAGARIDKSSRFTDWSNRPLTERQLQYSLMDVVPLRV
                     AYEKLATRLEKSGRSSWLAEEMGALTDLATYRQDPAESWRRFRLRSNNRKLLALVQAL
                     AAWREIAAQQRNLPRNRVLRDESVLEIAAHAPTTVAELARTRGLGRNLAEGKLGAEIL
                     AAIARVLATPERDYPDPPPQHEPARGIAPLVDLLRVLLKLRSEEHGVAQKLVADTEDL
                     ERIAGEDDPPVRALHGWRAEMFGKDALDLKHGRIALTASGKQVKLVRLAVASEAVQ"
     gene            11917..12411
                     /locus_tag="VGP50_01655"
     CDS             11917..12411
                     /locus_tag="VGP50_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEV8026101.1"
                     /translation="MKLTSPDFKAKGKIADEQTFNGFGCTGKNISPALNWSGTPPKGT
                     KSFALTVYDPDAPTGSGFWHWVVINIPATATSLPKNAGDLKSNLAPDGSIQTRTDFGA
                     PGWGGPCPPKGDKPHHYIFTLYAVDTDKLPVPGQDVSAAIVGFMLHFHKVGKPTTLTG
                     KYGR"
     gene            complement(12514..>13377)
                     /locus_tag="VGP50_01660"
     CDS             complement(12514..>13377)
                     /locus_tag="VGP50_01660"
                     /inference="COORDINATES: protein motif:HMM:NF044545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEV8026102.1"
                     /translation="VGGAWRALARIHMEQTRYPLHIIQQYTLPRGEAEDFLDIVARQS
                     RKSLEKIATVSRKRLDVVPIAARVLHRLLRRIAPRQLVFSAFGLREGHVYSLLDEAQQ
                     RQDPLLAACADMALARPRFGADGDELFAWTTALFADEDEAHRRLRRAAALLSDIAWHE
                     HPDYRADQALRYVLYMPVVGIAHDERAYLAATLNARYGGDGGSELLEHLLDEEALTRA
                     RRAGLALRLGYTLSGGVPGVLARNKLSLENGTLVLALSADGARRFGESVQRRLDALGR
                     SLGRRSDVRRV"
BASE COUNT         2227 a         4551 c         4424 g         2175 t
ORIGIN      
        1 ggcgtcggcg ccgcccgcgt gcgcgacctc ttcgagcagg cgcggctgaa ggcgcccgcc
       61 atcatcttca tcgacgagct cgacgcgctg ggccgcgcgc gcggcgccta tcccggcatg
      121 ggcggccatg acgagaagga gcagacactg aaccagctcc tggtcgagct cgacggcttc
      181 gattcacgct cgggcctggt gctgctcgcc gccaccaacc ggccggagat tctcgatccg
      241 gcgctgctgc gcgccggacg cttcgaccgc caggtgctgg tcgaccgccc ggacaaagcg
      301 ggccgcatcg atatcctcaa ggtgcatttg aagggtcaca cgctggcggc ggacgtcgat
      361 cccgagaaga tcgcggcgct cacgcctggc tttaccggcg ccgatctcgc caacctcgtc
      421 aacgaagcgg cgctcatcgc cacgcgccac aaggcggaca cggtgacgat ggacgacttc
      481 accaacgccg tcgagcgcat ggtggccggg ctcgagaagc gcaagcgcgt gctcaacgcc
      541 aaggagcgcc gcgtcgtcgc gcatcacgag atgggacaca cgctcgtcgc catgtcgctg
      601 ccgggcaccg ataccgtgca caaggtctcg atcattccgc gcggcatcgg cgcgctcggc
      661 tacaccatcc agcggccgag cgaggaccgc ttcatcatga cgcgcgagga cctcgaggac
      721 cgcatggccg tgctgctcgg cgggcgcgcg gccgagcata tcgtgttcca gcacttctcc
      781 accggcgccg ccgacgacct cgccaaagtg acgagcatcg cccgcgacat ggtggcgcgc
      841 tacggcatgg aggaaaagct cggccccgtg tcctacgacg cccagcgccc gaccttcctg
      901 caaggcgcgc ccccgcccga aggctggcac gagcggagct acagcgacga aaccgcgcac
      961 gccatcgatc aggcggtgca gcagatcgtc gagcgcgcct tcgcccgcac cgtggcgctc
     1021 ctcgagcgcc ggcgcgatct cctggagcgc ggcgccaagc tgcttctgga gaaagagacg
     1081 ctcgacgagg ccgacctcaa ggcgctccag gcggagctga ccgcgaagga cgccgtcgcc
     1141 gcgcagtaac ggtccctctc tccggcggag agagggacct gctccggaaa cgccgcttca
     1201 gtggttgtcg ctgccgctgg tgtcggtcga ggtcgagccg ccggccggcg tcgccgggtt
     1261 gctcgccagc tgccgctgcg tgatcacgcg cccggtatgc gcgtcgatgc gcagccggta
     1321 ggtcgcgttg ttgcggtccg cctgggccgt gtaggtgtcg ttcggctgcc ggtgcaactg
     1381 gctgatgttg tcatagccgt ccgagagcag cgtgtcgcgg atctggtccg atcgcatggc
     1441 ggcgggcggg gtcgagctgc cactcgccgc cgtgccgccg gcgagattca tcctcctgag
     1501 cgtcgcctca tcgaggctcg ccgtctcctg caggccatgg cggcgctgat aagcgctgag
     1561 cgcccgctcg gtctcggggc cgacgatgcc gtcgatcggg ccgttgtaaa agcggtcccg
     1621 ctgcagcttc agctgcgcgt cccgaacgag gccggaggcc tgtccaggtc cgggcgagga
     1681 aggcccgagg ccggcttcgt tgagggcgcc cgaggcgacg ttgtgctcgg cgccgatggc
     1741 attgaaggcc agctcgtcgg cgccttcagg catcgcccag ccgccagccg cgccgaccag
     1801 cacgcccacc gccgccccca ccgggccgcc gaccagagcg ccgacgccga ggcccgtcaa
     1861 gccgcccgta gcggcacgct gttgagtatt cgtgccgcag gccgccgtaa gcaggcccaa
     1921 ggcaatcacg gtaatggctc caatccgcat gggtgctccc cgatcccggt gattatcttc
     1981 ctgtctgtgt aacagcccga ttttcgccgg agttccggcg cttacgcttg acagcccggg
     2041 attggccgct attgtccgcg gcctccctgg ggagttccgg ccatggccaa atcgaacacc
     2101 atcctcatca agctggtgag cagcgccgac acgggatatt tctacgtcac gaaaaagaac
     2161 ccgcgggcga aaacggagaa gatggagctt cggaaatacg atccgatcgc ccgaaaacac
     2221 gtcgttttca aagaatcgaa gataaagtag cgcgatttcg cttaggaaat cgccgaagac
     2281 cgggtcaaag cgtccgccgg aaagctgaaa acgcaatctc tgccggcgcc tttcgcacta
     2341 tagagtgcct tgtcggcgcg ctgcagcagc gccccgccgg tgtcgccttt ttggcgggtc
     2401 atggcgatgc cgatgctgac ggtgacctgc ttctcgctcg cgccctcgcc gaggccgacc
     2461 ggcttgtcgg cgatcgtgtt gcgcagacgc tcggccacca gcgccgcgac cgagagcggc
     2521 gtgtccggca tgacgaccac gaactcctcg ccgccatagc gcgccaccag gtcgaagctc
     2581 cgcacatggc gctggatgcg ccccgcgatt tcgcgcagca ccgcgtcgcc cgcggcgtgg
     2641 ccatgcgtgt cgttgacgcg cttgaagtag tcgatgtcga tcatgagcag cgccgtgccg
     2701 ttctggccgt cggcggcgcg cgccatgaga ccgtcgagat gcgccatgag atagcggcga
     2761 ttgtagaggc cggtgaggcc gtcggtgagc gccagcgcga ggctgcgctg ataattgtcg
     2821 acgagcccgt cctgcaggcg cttccgccgg atctgcgtgc gcgcccgcgc cgtgagctcg
     2881 ttgcggtcga tcggccgcac gatgtagtcg ttggcgccga gatcgagtcc cttcgccagc
     2941 cgcggcacct cttcgccatc ggcgacgaga aggatcggca cgaagcgatg ggggtcggcc
     3001 gtgcgcaatt gggcgagaag gcgcaacgca tcctgctggc ccgcaaggct caagatcacc
     3061 aggtccaccg cggggtccat aagcgcgcgc agctcatcgg ggctcgaggg acgcagcact
     3121 tcggcggcca ccggcgccag catctcggtc aggcggtcgc cggcgaagcg attctcctcc
     3181 cacagcacga tccgcgccgg actcatatcc tcgctcggcc gcgcctcgcg cggccccgtc
     3241 tcgaagcggc catagacctc ttcgcgcagc cgccattcct cggtcatgcg cttcaagcgc
     3301 accagcgagc gcacgcgcgc aaagagcgcc acgtcgttga cgggcttggt caggaaatcg
     3361 tcggcgccgg cctggaggcc gcgcacgcgg tcggagactt ccgatagggc cgtcaccatg
     3421 atgacgggga tgtccgcggt cttcggatcg ctcttgaggc gccggcaaac ttcgaaaccg
     3481 tccatctccg gcatcatcac gtcgagcagc acgatgtcgg gccgctcgct cttcaccgcc
     3541 tgcagagcct gctcgccgct cgaggcggtg aggacggtga aatactcgct cgagagcttg
     3601 gcctcgagca gccggacatt gacgtcaacg tcgtcgacga ccaggacccg ggccgacatc
     3661 gctcagctcc agctcagctc aggaaccgtt cgacggtctg caggaaattg gcgacggaaa
     3721 tcggctttgc gatataagcc tcgcagccgc cttcgcggat cttctcttcg tcacccttca
     3781 tggcgaaggc ggtgacggcg atgatcggga tcgagcggag atcgtcgtcc tccttgatcc
     3841 acttcgtcac ctcgaggccg gagacctcgg gcaattgaat gtccatgagg atgaggtcag
     3901 gatgatgctg gcgggcgagc ttcagcgctt ccatgccgtc cttggtctgc agcgtcgcat
     3961 agccatgggc ttcgagcaga tcgtggaaaa gcttcatgtt cagatcgttg tcctcaacga
     4021 tcagaacccg cttgctcgac cgcttggtca ttggaacgtc cttgtaacca gggcaaccta
     4081 tcccgatccg tctcttatag tttgcataac cttaagactt cgtaactttt ggttacgtga
     4141 agcacggcgc ggcagtgggt ggagcgatgc gtatcggcgt ggatctcgga ggcaccaaga
     4201 tcgagctcgc ggcgctcgac cgcgacggcg cgattctgct gcgccgccgc atcgccacac
     4261 cggggaatga ctatgcggcg atcatccgtg ccgtgaccgc gctcgtcgac gcggccgagc
     4321 gcaccctcgg tcggcggggc accgtcggca tcggcatgcc gggcgcgctc tcgccggcga
     4381 ccggcctcgt caagaacgcc aattcgacct gcctcatcgg caagcccttc gaccgcgatc
     4441 tcatatcggc gctcggccgt cccgtgcgtt ttgccaatga tgccaactgc ttcgctctct
     4501 ccgaggcggt cgacggcgcg gctgcgggag cggcgatcgt gttcggcacc atcctcggca
     4561 ccggcgtggg cgcgggcatc gtggtcgacg gccgggtgct gaccggcgcc aacgccattg
     4621 ccggcgaatg gggccattcg ccgctgccct ggcccgaaga tgaggagcgg cccggccccc
     4681 gctgctattg cggcaagcat ggctgcatcg agagcttcct gtcggggccc ggactggctc
     4741 tcgaccatcg ctcggcgacg ggagaggcgc tcgaggcgac ggcgattgcc gccgccgccg
     4801 gcgccggcga tcccgaggcc gaagccaccc tcgcccgtta cgaggaccgg ctggcccggg
     4861 cgctggcgac ggtcatcaac ctgctcgatc cggacgtgat cgtgctgggc ggcggcttga
     4921 gcgccatcgc ccggctctac cagacggtgc cgcggcgctg gggcgcttac gtcttctcgg
     4981 acgcggtggc gacccgcctt gcgccgccac gccatggcga ttcgagcggc gtgcggggtg
     5041 ccgcctggct ctgggcgccg gacgaagcgc cgtgacgacg ctctgccgcg actgcgccag
     5101 tctcgccccg gcgccggcgg gtggccgctg tgcggcctgc ggctcgcccc gcctcatgtc
     5161 gcatccggag atcgaaaccc tgtcgctcgc gcatatcgat tgcgacgcgt tctatgccgg
     5221 cgtcgagaag catctcggcc gagacgacct tcacccatga catcgcggcg tatgaagcgc
     5281 ttgccgagga acttcggccg ctctgcgaca gcgtggcgca ctgcctgcaa cgggccaaca
     5341 aggccgcgac cggcgtcacg ctcaagctca agaccagtga tttccaggtg ccgagccgca
     5401 gccgcaagct cgccgatccc acgcggcgcg ccgagatcct ctatcgccga ccggctcgcc
     5461 gaagcggtgc tggcggatcc gcccgacctg ttcgccgggc ccacagaatg agcatgtgac
     5521 acgacacgcc gccgcgtgca cggcgccggc gatacagcac gagacgcgcg cgaccaccat
     5581 ggaacgcccg cgaatcatcg accaccgcgt gcaatcgcgc cgtgtgctac acttagccgc
     5641 tgttacgctc gccgacgatg gcacgaacat cttggagcag aggatgagca ggaggcgcga
     5701 gcgcaagggg cgcccgaagc gtccccgggg gtcgatgcgg aaagccgtcg ccgaggcaag
     5761 acaggccaat cgcttcctcg acgcgatcat cgagaacatt ccgcatatgg tcttcgtcaa
     5821 ggacgcccgc gacctccggt tcattcgctc caatcgcgcc ggcgagacgc tcctcggcgt
     5881 cgcgcgtacc gacctcgtcg ggaaaagcga ctatgatttc ttccccgaat ccgacgctgc
     5941 cttctttcag gccaaggatc gcgacacgct caaaggcgga acgctttccg atattccgga
     6001 agaagcgatc gacaccagga ccggccggcg ctggttgcac acgaaaaaag tgccgatctt
     6061 ggacagcacc ggcgcgccga aatacttgct cggcatctcc gaggacatca ccgagcacaa
     6121 gcggatggag gaagcgcgct cgcggctcgc cgctatcgtc gactcgtcgg acgatgccat
     6181 cttcggcaag tcctttgacg gtgtcattgc aacctggaac ggcgcggcgg agcgcctctt
     6241 cggtttcacg gcggacgaga tcgtcggcaa gctgatgtgg atcctcatcc ccgaacgtca
     6301 tcgcggcgag gaggcggcga tcttcgagcg gctgaagacg ggggagcgaa tcgagcccta
     6361 cgagacggcg cgcacgcgcc gcgacggcag cgaagtcgag gtgtgggtaa ccctttcctt
     6421 gatcaaagac caatcggcaa agaccatcgg catatcgacg atcgcccgcg acttcaccga
     6481 gatgaagcgc acccgccgag cgctcgtcga ggccaatgac gcggcggaag ccgccgcgca
     6541 ggcgctgcag caggcgcaga agcttcaggc gctgggccgc ctcgcggccg gtgtcgccca
     6601 tcacttcaac aacctgctca ccgtcgtgat cggcaacctc gaggaaggga cggcgcgcca
     6661 gagcgcggcc ggactggcgc gcgagcctat gctgacggcc gcactcagcg cggcgctacg
     6721 cggttcgggg ctgacccggc aactcctcag cttctcgcgc cagcagttgc tccagccgga
     6781 gatcatcgag ccctcgaagc gcctcgccga taccgcgttt ctcttcgccg catcgctgcg
     6841 caaagacatc accatcgaga ccgatattcc cgaccatctc tggccgatcg tcgtcgatcc
     6901 ggccgagctc gagctggcgc tcctgaacct cgccctaaat gcccgcgacg ccatgcccgc
     6961 gggcggcgtg ctccgcctca ccgccgtcaa ccagaccatc gagggcgcgc ggccgggcct
     7021 tgccggccct tatctggtta tcgggctcag cgacaatggc acggggatct cgcccgagat
     7081 cctgccgcgg gtcttcgacc ccttcttcac caccaaggat gtcggttccg gcagcggact
     7141 cggcttgagc caagtccatg gcttcgctca ccaatccggc ggcacggtcg gcatcgagac
     7201 cacgccgggc aaagggacga ccgtccgcct ctatctgccg gcatcgcccg gcgcgaggca
     7261 agcggcgccg gccgccgagg catctgccta ttgccgtgcc ggcgcgggga cggttctcgt
     7321 cgtcgaggac gacgccgatg tcgccgcgct cgccgccgag tcattgcggc atcggggctt
     7381 ccgcgtgaag ctcgccgatc gagcccaggc ggccctcgac cttctgtcgc agggcgagac
     7441 ggtggatttg attttctccg atatcgtcat gccaggcggg atgaacggcc tcgagctcgc
     7501 agaagcggtg cgggcgcgct ttcccacgat cccggtgttg ctgacgaccg gccatagcga
     7561 cgccgtgacg gacgccatga cgcgggggct gcagatcatc gccaaaccct atcgcctggc
     7621 ggaaatctgc gatcgcgtcg cccgcctgat ccgagcgacg acagtggccg gagcctaacc
     7681 gcgactagca attcgggcgc ggcagtgcct cgatctcgtc gagcttggcg cggatgacgt
     7741 agtcgctgta gtcgagcgcc atgtcctgag agacgccatt gtcgaggagc cgcatgccga
     7801 gctcgtagtc cggctgctcc accttggtgt cgggcgggaa gaacgccagg cggatgcgcc
     7861 aggacgggcg attgaggagc gggctgtcca ccggcttctc gccggctttc ggggcatcgg
     7921 gcttgagttc cgggccgatg acggccgtga tctgcgaggc gttctcgaca gaggcgccgt
     7981 cgaagaccgc cttggcgacg aattgctcgc cagcctgcgc ccggtcgatc aggagcagcg
     8041 tgtgtgccgt cgggaagagg acccccggcg ccagcgtcag cgtgctcgcc tcgggacggg
     8101 tgaattcggc ggtcccgccc ttccctgcgc cgtcgagccg cgcctcaccc ttgatctcct
     8161 cgtcgggctc gccgttgcgc aagcggcgct cgttgaagcg atagcgcaat ccgtccttcg
     8221 actcccaggt cacgaggctc gagctcacct cgacgccgtc ctgctcggcg tattgcaggc
     8281 gcaaccggaa gcgctgctgc accgtccagc cgtcgcaagt ctcgccccat tcataggcca
     8341 tggcgccggt ggcggcgacg acgccgctgc tctgcttggc ggagccgagg gtgaggctgt
     8401 agagcgctcg atgcggggcg atctccgcgg ccgatgcggg caacgcggcg acgcagaagc
     8461 ccgcggcaac tccgatgagg cgtagagacc aggaaatcat cacatcgacc tcgcgcgagc
     8521 ccgaaccggc tcgacctcta tgtcacgtcc ctcgcgttaa caatcagtat agcatgcctg
     8581 tggcccgcgt atccccgccg ggcgattcgt cagctgctct tgctcttggt ctccgggggg
     8641 agtgccaggc ggatatgcag ctccttgagg cgctccggcg ggacggctgc cggcgcctgc
     8701 atgagcaggt cctgcgcctg ctgggtcatc gggaaggcga cgacctcgcg gatattgggc
     8761 gtttccgcca agagcatgac gatgcggtcg atgccgggcg ccgagccgcc gtgaggcggc
     8821 gcgccatatt tgaaggcatt gagcatgccg ccgaaacgcg cctcgacctc ggccgccgcg
     8881 tagccggcga tctcgaaggc cttgtacatg atatccgggc gatggttgcg gatggcaccg
     8941 gagctcagct cgaccccgtt gcagacgatg tcgtattgga aggcgttgat ctcgagcggg
     9001 tccttggtca acagcgcctc gagcccgccc tgcggcatcg agaatgggtt gtggctgaag
     9061 tcgatcttct tttcttcctc gttccactca tacatcggaa aatcgacgat ccagcagaaa
     9121 cggaagtcgt tcttggcgat gaggttgagc tcctgcgcga tcttggtccg cgcggcgccg
     9181 gcgagcttct ctgccgcggc cttcttgtcg cagacgaaga atacggcatc gccgtctttg
     9241 agaccggcca gcgtcttgag ctgctgcagc cgcggctcgt ccaggaattt ggcgatcgga
     9301 cccttggccg ccccttcggc gaagatgatg tagccgagcc ccggcgcacc ctcgccgcgt
     9361 gcccaatcat tgagcttgtc gaaaaagctc cgcggctgag cggcgacgcc gggcacggcg
     9421 atcgcgcgca cgaccgcgcc ttcggccacg gcgccggcaa agaccttgaa gcccgagccg
     9481 cggaacacgt cccgcacctc gctgatcagg agcgggttac gcagatccgg cttgtcgctg
     9541 ccaaatcgca gaagcgcgtc ctcataggtg aggcgcggga aaggcggcgc ggtcacgggc
     9601 ttgccgccgc cgaactcggc gaagacaccg gcaagcaccg gctcgatcgc ggcgaagacg
     9661 tcttcttgcg tgacgaagga catctcgaaa tcgagctggt agaactcgcc gggcgagcgg
     9721 tcggcgcggc tcgcttcgtc acgaaagcag ggcgcgatct ggaaatagcg atcgaagccc
     9781 gcgaccatga gaagctgctt gaattgctga ggcgcctgcg gcagcgcgta gaacttgccc
     9841 ggatggagcc ggctcggcac caggtagtcg cgggcgcctt cgggcgagct ggcggtcagg
     9901 atcggcgtct ggaactcggc gaagccctgc tcgatcatcc gccggcgcag cgaagcgata
     9961 acattcgagc gaagcacgat gttcgcgtgc atgttctcgc ggcggaggtc gaggaagcgg
    10021 tatctgagcc gcatctcctc ggggtagtcg gcgtcggcgt tgaccgggaa gggcagcggc
    10081 tcggcttcgg agaggacttg gagctcggcg atctgcagct cgacctcgcc ggtcggcaat
    10141 ttgggattga ccgtctcggg cgagcgcttc accaccgtgc cggtgacggt gatgacgctc
    10201 tcgagccgct gcgcatcggc cagtggaaag agcgggctgg cgctgtcgat cacgcattgg
    10261 gtgatgccgt ggtgatcgcg caggtcgatg aacagcagct ggccatggtc gcgcttgcgg
    10321 tggacccagc ccgaaaggcg ggcgacgcgg ccggcatcgc cgaggcgcag agccccgcag
    10381 ctatgggttc ggtaggcatg catcggcgtt tgtccgctgg tcgttccgaa ggccggcaaa
    10441 agcacatgga agcgcggcct ttgtcaagcg atgccggcct cctattgttc tgtccgttta
    10501 gggtgtcggc accctcgaaa atgcttgtga cacgtccgct ttcccggttt atagcacggc
    10561 gatgacactg atcgacaaca ccgaggcgct tgccgccttc tgccgacgcc aggccggtgc
    10621 cggctatgtc accgtcgaca ccgaattcat gcgcgacaag acctactggc cgcagctctg
    10681 cctggtgcag atcgccggac ccgacgaagc cgccgccatc gatccgctgg cgcccggcat
    10741 cgacctctcg ccgctcttcg agctcatggc cgactccagg ctcctcaagg tcttccatgc
    10801 cgcgcggcag gatatcgaga tcttcgtcaa tctgacgggc gccgtgccgg cgccgctctt
    10861 cgatacgcaa gtcgcggcca tggtctgcgg cttcggcgac gccgtgagct atgagacgct
    10921 ggcgagccgg ctcgccggcg cccggatcga caagtcctcg cgcttcaccg actggtcgaa
    10981 ccggccgctc accgagcggc agttgcaata ttcgctgatg gacgtggtgc cgttgcgcgt
    11041 cgcttatgaa aagctcgcga cgcggctgga gaagagcggc cgctcgtcct ggctcgccga
    11101 agagatgggc gcgctcaccg acctcgcgac ataccgccag gacccggccg agtcctggcg
    11161 gcgcttccgc ctgcgcagca acaaccggaa gctcctggcg ctggtgcagg cattggcagc
    11221 atggcgcgag atcgcggcgc agcagcgcaa cctgccgcgg aaccgcgtgc tgcgcgacga
    11281 atcggtgctg gagatcgccg cgcatgcgcc gaccaccgtc gccgagctcg cccgcacgcg
    11341 cggcctcggc cgcaacttgg ccgagggcaa gctcggcgcc gaaatcctcg ccgccatcgc
    11401 ccgcgtgctt gcgacgcccg agcgcgacta tcccgacccg ccgccgcagc atgaaccggc
    11461 gcgcggcatc gctccgctgg tcgatctgct gcgcgtgctg ttgaagctgc gctccgagga
    11521 gcatggcgtc gcccagaagc tcgtcgccga caccgaggat ctcgagcgaa tcgccggcga
    11581 ggacgacccg ccggtgcgcg cgcttcacgg ctggcgcgcc gagatgttcg gcaaggacgc
    11641 actggacttg aagcatggcc gcatcgcgct gaccgcctcc ggcaagcagg tcaagctcgt
    11701 ccgccttgcg gttgcgagcg aggccgtgca atagccgaag cgtcctccgg cttgacgggt
    11761 tgtcaccgca ccctattctg cggcgcccga gggcgtcctg ccctcatcga cagaacgtca
    11821 cgcaactcag ggggaagcag aatgattcga ggaatgatgg ttgccgcggc ggcgctcgcg
    11881 ctcgcggcct tcgccgccgc gccggcgagc gcggccatga agctgaccag ccccgatttc
    11941 aaagccaagg gcaagatcgc cgacgagcag acctttaacg gtttcggctg caccgggaag
    12001 aacatctctc cggctctcaa ctggagcggc acgccgccca agggaaccaa gagcttcgca
    12061 ttgacggtct atgatcccga cgctcccact ggcagcggct tctggcattg ggtcgtgatc
    12121 aacattccgg cgaccgccac gtcgctcccc aagaacgcgg gcgacctcaa aagcaatctc
    12181 gcgcctgacg gcagcatcca gacccgcacc gatttcggcg cccccggctg gggcggccca
    12241 tgcccgccca aaggcgataa gccgcaccac tacatcttca cgctctacgc cgtcgatacc
    12301 gacaagctgc cggtcccggg tcaggacgtc tccgcggcga tcgtcggttt catgttgcat
    12361 ttccataagg tcggcaagcc aacgacgctc accggcaaat acgggcgctg acgcggcact
    12421 cggccacccg gctcccactc cactcggatg cgcgtggagt gggtcccggg tcgcggatat
    12481 cagagcgcgc tgaagcccag agtggttccg gttctacacc cgccgcacgt cgctgcggcg
    12541 gccgaggctg cggccgagcg cgtcgaggcg gcgctgcacg ctttcaccga aacggcgcgc
    12601 gccatcggcg gacagggcga gcacgagtgt gccgttctcc aggctcagct tgttgcgcgc
    12661 cagcaccccg gggacaccgc ccgagagcgt gtagccgagt ctgagcgcga gacccgcgcg
    12721 ccgcgcccgc gtcagcgcct cttcgtcgag caggtgctcc aagagctcgc ttccgccgtc
    12781 gccgccatag cgcgcgttga gcgtcgccgc gagataagcc cgctcgtcgt gagcgatgcc
    12841 gacgaccggc atatagagga cataacgcag cgcctggtcg gcgcgatagt ccgggtgctc
    12901 gtgccaggcg atgtcgctca gcagcgccgc cgcccggcgc aggcggcgat gcgcctcgtc
    12961 ctcgtcggcg aagagcgcgg tggtccaggc gaagagctcg tcgccatcgg cgccgaagcg
    13021 cggccgcgca agcgccatgt cggcacatgc ggcgagcagc ggatcctggc gctgctgggc
    13081 ttcgtcgagc aggctgtaga catgcccctc gcgcaggccg aacgccgaga agacgagctg
    13141 ccgcggcgcg atgcgccgca agagccggtg caggacacgg gcggcgatcg gcaccacgtc
    13201 gaggcgcttt cgcgagaccg tggcgatctt ctccaacgat ttgcgcgatt gccgcgccac
    13261 gatgtcgagg aaatcctcgg cctcgccgcg cggcagcgtg tattgctgga tgatatggag
    13321 aggatagcgc gtctgctcca tatggatgcg ggcaagcgcc cgccatgcgc caccgac
//
LOCUS       DASYNS010000053         4862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_638184, whole genome shotgun sequence.
ACCESSION   DASYNS010000053 DASYNS010000000
VERSION     DASYNS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638184"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VGP50_01665"
     CDS             complement(<1..1166)
                     /locus_tag="VGP50_01665"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HEV8026103.1"
                     /translation="MNRFAALLDALLFTPSRLGKLRLMTEYFAGTGDPERGWALAALA
                     GELSFAEVKASQIRDLAESRVDAELLAWSYDFVGDLAETVSLIWPARAGFERVPDLSE
                     IVAELDAATRANAGERLARWLDALDATGRWALLKLITGGLRIGVSARLAKTALAEWGK
                     VPLDEIEALWHGLLPPYVELFAWLDGKGPRPDIGSRPTFHPLMLANPLDDADLDALDP
                     SAYRAEWKWDGIRVQLVSRGGETRLYSRSGDDIGTAFPDILEAMPHDVVLDGELLVLR
                     DGEVAPFNDLQQRLNRKTASAKMLGEYPAAVRLYDILEEGGEDFRPLPFDERRRRLER
                     WYSAGPRPRLDLSPLVPFGNWDELRGLREGARERGIEGLMLKRRDSAYMPGRPKG"
     gene            complement(1163..2179)
                     /locus_tag="VGP50_01670"
     CDS             complement(1163..2179)
                     /locus_tag="VGP50_01670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEV8026104.1"
                     /translation="MTTSASPHSWLKPTAQGLYCEAGDFFIDPVVPVPRAVITHGHGD
                     HARPGNGQVLATQETLAIMRRRLGDDAGGTLQAQGYRQPIRVGDVTLRLLPAGHVLGS
                     AQVVLEHGGARVTVSGDYKRRFDPTCVPFEPEPCDVFVTEATFGLPVFRHPPDAQEIE
                     KLLHSMRLYPERCHLIGVYPLGKCQRLLSLLRRRGYGEPVYLHGALMGLCALYEEHGI
                     ALGPLLPATGPSRDALKGRLVLAPPGAFAEIWSRRLPDPVAAIASGWMRVKQRAKASG
                     VELPLVISDHADWDELWATIEEVGASEIWITHGTEEAMLRQATLKGLKARALSLRGFE
                     DDEQ"
     gene            complement(2286..3116)
                     /locus_tag="VGP50_01675"
     CDS             complement(2286..3116)
                     /locus_tag="VGP50_01675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8026105.1"
                     /translation="MLLGVLVATNLVTAAVLALALRRLAIHRARRRQRNIFASWPIPS
                     VVPGDIAPGLSAGPLGPSAASEIVAVANYRVPGGTSDLETWVLCNLAKRARLVFEFGT
                     ATGKTTYLLARNAPADAEIVTLTLGATDAYGVEARDAPDAKRAALDESLDAFVYRGTP
                     VAHKIRQLFVDSKGFDETPYVGRCDLIFVDGSHARSYVESDSRKALAMLGPGGYVLWH
                     DYAGPRHATGVYEALNALAQELPLRHIKGTMLVAYQKPKDRPSSLAGEGGARREASGG
                     "
     gene            complement(3154..4596)
                     /locus_tag="VGP50_01680"
     CDS             complement(3154..4596)
                     /locus_tag="VGP50_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015074073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8026106.1"
                     /translation="MEGARQAVRTDVPARLDRLPWSPFHRLVIVALGITWILDGLEVT
                     LAGSIAAALQQSPRLHLSAEEVGLTGTAYLTGAVLGALFFGYLTDRLGRKRLFNVTLG
                     VYLLATALTALSWDFSSFAIFRFFTGAGIGGEYTAINSAIQELIPARLRGRTDLAING
                     SFWIGAALGAFGSIWLLDPALLPPELGWRCAFGIGAVLGLVILYLRRFVPESPRWLMI
                     HGRIAEAEATVTEIESRVGAGTGVALPPACGRVSLVATGGVSLAVLAQTLLRAYPRRT
                     LLGIVLMASQAFFYNAIFFTYALVLTRFYGVPAAHVGWYILPFALGNFAGPVLLGPLF
                     DTVGRKPMIAGTYALSGLLLALTGALFAAGVLDATGLTIAWTVVFFFASAAASAAYLT
                     VGESFPLELRALAIALFYAFGTLLGGVLSPWLFGVLIASGSRGAIFGGYLLGAVLMLG
                     AAVIELKLGIAAEGRSLEDVAAPLSLEPHG"
BASE COUNT          866 a         1650 c         1650 g          696 t
ORIGIN      
        1 cctttcggcc ggcccggcat ataagcggaa tcgcggcgct tcagcatgag tccctcgatg
       61 ccgcgctcgc gcgctccctc gcgcaatccc ctgagctcgt cccagttccc aaacggcacc
      121 agcggcgaga gatcgaggcg cggccgcggt ccggcgctgt accagcgctc gagccgccgg
      181 cggcgctcgt cgaagggaag cggccgaaaa tcctcgccgc cttcctcgag gatgtcatag
      241 agccgcacgg ccgccggata ctccccgagc attttcgccg acgcggtctt gcggttgagc
      301 cgctgctgca gatcgttgaa aggcgcgact tcaccgtcgc gcaacaccag aagctcgccg
      361 tcgagcacga cgtcatgggg catggcctcg aggatgtcgg gaaaggctgt gccgatgtcg
      421 tcgcccgagc gcgaatagag ccgcgtctcg ccgccccgcg acacgagctg cacgcggatg
      481 ccgtcccatt tccattcggc gcggtaagcg gaaggatcga gcgcatcgag gtccgcgtcg
      541 tcgagcgggt tcgccagcat cagcggatgg aaggtgggcc ggctgccgat gtcggggcgc
      601 ggtcccttcc cgtcgagcca ggcgaaaagc tcgacatagg gcggcagcag cccgtgccaa
      661 agcgcttcga tctcgtcgag cggcactttg ccccattcgg cgagcgccgt cttggcaagc
      721 cgcgccgaga cgccgatgcg caagccgccg gtgatcagct tcaagagcgc ccagcgtccc
      781 gtcgcatcga gcgcatcgag ccagcgcgcg agccgctcac cggcattggc acgcgtcgcg
      841 gcgtcgagct cggcgacgat ctcggaaagg tcgggcacgc gctcgaagcc ggcccttgcc
      901 ggccagatga gcgacacggt ctcggcgaga tcgccgacga aatcatagga ccaggccagg
      961 agctccgcat cgacccggct ctccgcgagg tcgcggatct gcgacgcctt cacctcggcg
     1021 aaactcagct cgcccgcgag cgcagcgagt gcccagccgc gctcgggatc gcccgtgccg
     1081 gcgaaatatt cggtcatgag ccgcagcttg ccgagccgcg acggcgtgaa cagcaaggca
     1141 tcgaggagcg ccgcgaaccg gttcactgct cgtcgtcctc gaatccccgg agcgacaagg
     1201 cgcgcgcctt gaggcctttg agcgtcgctt ggcgcagcat cgcctcttct gtgccgtggg
     1261 tgatccagat ctcgcttgcg ccgacttcct cgatcgtcgc ccagagctcg tcccagtcgg
     1321 cgtgatccga gatcaccagt ggcagctcga cgccgctcgc cttggcgcgc tgcttgacgc
     1381 gcatccagcc cgacgcgata gcggccacgg gatcaggcag ccgccgcgac cagatctcgg
     1441 caaaagcgcc gggcggcgcc aggacgaggc ggcccttgag cgcatcgcgc gacgggccgg
     1501 tcgccgggag caacggaccg agtgcgatgc catgctcctc gtaaagcgcg cacagcccca
     1561 tgagcgcgcc atggagatag acgggctcgc catagccgcg gcgtcgcagc agcgacagca
     1621 ggcgttggca tttgccgagc gggtagacgc cgatgagatg gcagcgctcg ggatagagcc
     1681 gcatcgaatg cagcagcttc tcgatctctt gcgcatcggg cggatggcgg aacaccggga
     1741 gcccgaaagt cgcctccgtg acgaagacgt cgcagggctc gggctcgaac ggcacgcagg
     1801 tcggatcgaa gcggcgcttg tagtcgccgg aaacggtgac gcgcgcgccg ccatgctcga
     1861 gcaccacttg ggcgctcccc agcacatgcc ctgccggcag cagccgcagc gtaacgtcgc
     1921 cgacgcggat cggctggcga tatccctgcg cctggagcgt gccgccggca tcgtcgccga
     1981 gccggcggcg catgatggcg agcgtctctt gtgtcgccaa cacctggccg ttgccgggcc
     2041 gcgcatggtc gccatggcca tgcgtgatga cggcacgcgg cactgggacg accggatcga
     2101 tgaagaaatc ccccgcctcg caataaagcc cttgggccgt cggcttgagc cagctgtgcg
     2161 gtgatgcgct tgtcgtcatg acatcgaggt aggttcccct ctccccttgc gggagaggga
     2221 ggggcccgtg gcgcagcaac gggagggtga ggggaatggt tcagcgtcct gctgcgccag
     2281 ccccctcacc ctcccgacgc ttcgcgtcgg gcccctccct ctcccgcaag ggaagaggga
     2341 cgatccttcg gcttctgata cgccaccagc atcgtgcctt tgatgtggcg cagcggcagc
     2401 tcttgcgcca gggcattgag cgcctcgtag acgcccgtcg catggcgggg ccccgcatag
     2461 tcgtgccata gcacgtaacc cccggggccc agcatggcga gggctttgcg gctgtcgctt
     2521 tcgacatagg agcgcgcatg cgagccgtcg acgaaaatca gatcgcagcg tccgacatag
     2581 ggagtctcgt cgaagccctt gctatcgacg aagagctgcc ggatcttgtg cgcgaccggc
     2641 gtgccccgat aaacgaaggc gtcgagcgat tcatcgaggg cggcgcgttt ggcatcgggc
     2701 gcgtctctcg cctcgacgcc gtacgcatcc gttgcgccga gcgtgagcgt cacgatctcg
     2761 gcatcggccg gcgcgttccg cgccagcaga tacgtggtct tgccggtcgc cgtgccgaac
     2821 tcgaagacga ggcgcgccct cttggcgagg ttgcacagca cccaggtctc gagatcgctg
     2881 gtgccgccgg ggacgcggta gttggcgacg gcgacgattt cgctcgccgc cgaagggccg
     2941 agcggtcccg cgctcaggcc cggcgcgatg tccccgggaa cgacgctcgg aatcggccag
     3001 ctcgcgaaga tgttgcgctg tcgccgcctt gcccggtgaa tcgcgagacg ccggagcgcc
     3061 agcgccagga ctgctgccgt caccagattc gtcgccacca gtacgcccag cagcacgcat
     3121 cgctccccag gccgtcggcg gttccgttct agctcagccg tgcggctcga gcgagagcgg
     3181 cgccgccacg tcctcgagcg agcggccttc ggcggcgatg ccgagtttga gctcgatcac
     3241 ggcagcgccg agcatcagca ccgcgcccag cagatagcca ccgaagatcg cgccgcggct
     3301 gccgctcgcg atcagcacgc cgaagagcca tgggctcagc acgccgccca ggagcgtgcc
     3361 aaaggcatag aagagcgcga tggcgagagc gcggagctcc aggggaaagc tctcgccgac
     3421 ggtgagatag gcggcgctcg ccgccgcgga ggcgaagaag aagaccaccg tccaggcgat
     3481 ggtgaggccg gtggcgtcga gcacgcccgc agcgaagagc gcgcccgtca gcgccagcag
     3541 caatcccgag agcgcatagg tgccggcgat catcggcttc cgcccgaccg tgtcgaagag
     3601 cgggcccagg agaaccggcc ccgcgaaatt gccgagcgcg aagggcagga tataccagcc
     3661 gacatgagcg gccggcacgc cgtagaagcg cgtcagcacc agggcatagg tgaagaagat
     3721 ggcgttgtag aagaaagctt gcgacgccat cagaacgatg ccgaggagag tgcgtctggg
     3781 ataagcgcgc agcaatgtct gcgccagcac cgcgagcgac acgcctcccg tggcgaccag
     3841 cgacacgcgg ccgcatgccg gcggcaacgc gacccccgtg ccggcgccga cgcggctttc
     3901 gatctccgtg accgtcgcct cggcctcggc gatgcggccg tggatcatca gccaacgcgg
     3961 actctccggc acgaagcggc gcagatagag gatgacgagg ccgagcacgg cgccgatgcc
     4021 gaaagcgcag cgccagccga gctccggcgg cagcagcgcc ggatcgagca gccagatcga
     4081 gccgaacgcg ccgagcgcgg cgccgatcca gaagctgcca ttgatcgcga ggtccgtgcg
     4141 cccgcgcaac cgcgcgggga tcagctcctg gatcgccgag ttgatcgcgg tgtattcgcc
     4201 gccgatgccg gcgccggtaa aaaagcggaa gatggcgaag ctcgaaaaat cccaggagag
     4261 cgcggtcagc gccgtcgcca ggagatagac gccgagcgtc acgttgaaaa gacgcttgcg
     4321 gccgagccgg tcggtgagat agccgaagaa caacgccccc agcaccgcgc cggtaagata
     4381 ggcggtgccg gtgagcccga cctcttcggc ggagagatga aggcgcgggc tctgctgcag
     4441 cgcagccgcg atcgagcccg ccagcgtcac ctcgagcccg tcgagaatcc aggtgatgcc
     4501 gagcgccacg atcaccaggc ggtgaaacgg gctccagggc agccggtcga gccgcgccgg
     4561 cacatccgtc cgtactgcct gtcgcgcgcc ttccattccg cccgctcccg ctttgtcacg
     4621 tcagatgaac gcccggggcc ggtttcgatg ccgtcggcgg cggcgatcca tggctcggaa
     4681 gagcgagcat gaacttgatg gtcgtcatcc cctgtcctga cgagcgaggg accacccgaa
     4741 cgccggagaa tgcctggcgt catcagcaat ccgtcaagaa atcgtcatcg agttccacaa
     4801 cgtgcacact ggtcgcatca cattcatcac gtgacttggt cgaggagcga accctttgtt
     4861 aa
//
LOCUS       DASYNS010000054         5066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_794196, whole genome shotgun sequence.
ACCESSION   DASYNS010000054 DASYNS010000000
VERSION     DASYNS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794196"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VGP50_01685"
     CDS             complement(<1..725)
                     /locus_tag="VGP50_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV8026107.1"
                     /translation="MAAGEGSSERQRPGTDVSVSLRMLVLIRWVAVVGQGATILFVHY
                     LLGFTLPVKSAMGVVGTSAALNILASWPRRSTARLRDREAALYLGYDILQLGMLLFLA
                     GGLENPFSILMLAPVMVSATILSRRSVVWLSTLTVLAISTLAVFHLPLPWINAPPPPS
                     TLYVLGVWVALVSSTIFIAGYTWSIAQEARRMRDAFTATQLALAREQRISAVGGIAAA
                     AAHQLGSPLATIAVVAKELVRELP"
     gene            867..1625
                     /locus_tag="VGP50_01690"
     CDS             867..1625
                     /locus_tag="VGP50_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8026108.1"
                     /translation="MSDPVVQVSDLRKSFGDTLAVDGIDFVVPRAATAALLGGNGAGK
                     TTTLSMLLGLLLPTAGRIRIFGEDMLRHRYRVLPRMNFSSPYVDLPHRLTVRQNLSVY
                     GRLYGLAQLKTRIETLASDLQIGEFLDRQTGQLSAGQKTRVALAKALLNEPELLLLDE
                     PTASLDPDTGDWVRGYLEIYQRRTGATILLASHNMGEVERLCSEVIMMRRGRIVDRGS
                     PRALIERYGRDTLEQVFLDIARAGTRFDSREAAQ"
     gene            1688..2455
                     /locus_tag="VGP50_01695"
     CDS             1688..2455
                     /locus_tag="VGP50_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8026109.1"
                     /translation="MVLRYLYLLRSSWPRTLELLYWPAIQMILWGFTSKFLMTNSSYV
                     ARAGGVLLAAVMLWDVMFRGQLGVSVSFLEELWSRNLGHLFVSPLRPYEWVLSLLTMS
                     LVRVIIGVVPAALLAIPLYHYSIFTLGPPLIAFFVLLLIMGWGLGLMIGALLLRHGLG
                     AESLAWLAVFLLAPASAVYYPVSVLPPWLQYLAWALPSAHVFEGMRAVMFEGVFRVDH
                     FMAAAALDLVYLLFGGAIFLYAFRQARQRGALLQMGE"
     gene            2460..3908
                     /locus_tag="VGP50_01700"
     CDS             2460..3908
                     /locus_tag="VGP50_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEV8026110.1"
                     /translation="MAFPRSTRRQAIIGGAGLLATAAAGGAIYAAWPRGPRLVAGRRM
                     LEVDGKAAAVLGLEHRGAALSLVQGGRFQVALENRLDEPTLVHWHGLTPPSAQDGTPG
                     LSQPALAPGGRYDYDFPLQRAGTFWMHSHQGLQRAKLLAAPLIIADPADARRDEQDVV
                     MFLADFSFRAPEELLAELTRGQAAKHDMPGMDHGPMDHAEHVAGQDDAPAERVHANDI
                     EFDAYLANDRNFADPDIVRVEPGGHVRLRVINGAAATNFWIAVDGNLRSELIAVDGNP
                     IVPREGRRFGLAMAQRIDLRLSLPEGEGAYPIYAVRENAVQRTGIILATAKGEVKKLG
                     AAQRLPTSRLDVALEGLLAAAEPLAERVVDRTLSADLTGDMASFVWGINGKSYGEDTP
                     LLVRAGERVELVMQNRTNMAHPMHLHGHRFQVTAIGDRRLAGAVRDTVLVPVGTSVTV
                     AFDADNPGKWAFHCHNEYHMAAGMMTSVQYES"
     gene            complement(4005..4613)
                     /locus_tag="VGP50_01705"
     CDS             complement(4005..4613)
                     /locus_tag="VGP50_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEV8026111.1"
                     /translation="MITAFRRRSVFAAMLLCLTAPGAALRGPSALAHTASEAPPAAMA
                     DIAGHFALTTADGRSVTDESYRGKWLVIYFGYTSCPDACPLTLNNIGVALDKLGPLAD
                     KVQPLFITVDPEHDTRAAMTQYLKSFDPRIVGLSGTPEEIAATANQYHVYFRTRQLGN
                     GEYTVDHNSLIYVIAPDGQFAKLLTPDLPGHELADQLHALIQ"
     gene            complement(4683..>5066)
                     /locus_tag="VGP50_01710"
     CDS             complement(4683..>5066)
                     /locus_tag="VGP50_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV8026112.1"
                     /translation="NAVVQNTANCTGCGGQWNGKETAPVGSFAANGFGLYDMAGNVWE
                     WTQDCYHDSYNGAPTDGSAWTSGDCYQRVLRGGSWINTPKGLRAALRDWIGLDNRVNL
                     FGFRVGRTLGAVDVQPRGAVAPRRD"
BASE COUNT          759 a         1708 c         1735 g          864 t
ORIGIN      
        1 ggcagctcgc gcaccagctc cttggcgacg acggcaatgg tggcgagcgg gctgcccagc
       61 tgatgcgccg cggcggcggc gatgccgccc accgccgaga tgcgctgctc gcgcgccagc
      121 gcgagctgcg tcgcggtgaa ggcgtcgcgc atgcgccgcg cttcctgggc gatgctccag
      181 gtatagccgg cgatgaagat cgtcgaggag acgagcgcca cccagacgcc cagcacgtag
      241 agcgtcgagg gcggcggcgg cgcgttgatc cagggcagcg gcaggtggaa cacggcgagc
      301 gtgctgatcg cgagcaccgt cagggtcgag agccatacca cgctccggcg cgacaggatg
      361 gtggcggata ccatcacggg cgccagcatc aggatgctga agggattctc gaggccgccc
      421 gccaggaaca gcagcatgcc gagctgcagg atgtcgtatc ccagataaag cgccgcctcg
      481 cggtcgcgga gccgcgccgt gctgcgccgc ggccagctcg cgaggatgtt gagcgccgcc
      541 gaggtgccga ccacgcccat cgcgctcttg acgggcagcg tgaagcccag gaggtagtgc
      601 acgaagagga tcgtcgcgcc ctggccgacc accgccaccc agcggatcag cacgagcatg
      661 cgcaggctga ccgagacgtc cgtgcccgga cgctgcctct cgctcgagcc ttcgcctgcg
      721 gccatgcgcc gtcgctcctc gatccgccgc agcgccgctt cgaccgcggc gcgacaatgc
      781 ccattatacg cgaggccgcc gccggcgggg aggctccgga ccttcgcgtc tggccggttc
      841 gcgacgatta cgccatagtg tgccgcgtgt ccgatccggt cgtccaagtc agcgatctcc
      901 gcaagagctt cggcgacacg ctcgccgttg acggcatcga cttcgtcgtg ccgcgcgccg
      961 cgactgctgc gctcctcggg ggcaatggcg ccggcaagac gaccacgttg tcgatgctgc
     1021 tcggtctgct gctgccgacg gcgggacgca tccggatctt cggcgaggat atgttgcgcc
     1081 atcgttaccg ggttttgccg cgcatgaact tttcgtctcc ttatgtcgac ctgccccatc
     1141 gcctgacggt gcgccagaac ttgtcggtgt atgggcgact ctacgggctc gcacaactca
     1201 agacgcgcat cgagacgctg gcgagcgacc tgcagatcgg cgaatttctc gaccggcaaa
     1261 ccggtcagtt gagcgccggg cagaagaccc gcgtggcgct tgcaaaagcg ctgctcaacg
     1321 agcccgagct cctgctcctc gacgagccga cggcgtcgct cgatcccgac accggcgact
     1381 gggtgcgcgg ctatctcgag atctaccagc ggcggacggg cgccaccatc ctgctcgctt
     1441 cccacaacat gggcgaggtc gagcggctct gctccgaggt gattatgatg cgccgtggcc
     1501 gcatcgtcga ccgcggcagc ccgcgcgcgc tcatcgagcg ctatggccgc gacacgctcg
     1561 agcaggtctt cctcgacatc gcgcgcgccg gcacgcgctt cgattcgcgc gaggcggcgc
     1621 agtgagcgcg acggccgtcg atcaccgcgc cgcgctccaa cgcttctcgc cggcgcggct
     1681 cggcgccatg gtgctgcgct atctctacct gctgcgcagt tcctggccgc gcacgctcga
     1741 gctgctctac tggccggcca tccagatgat cctctggggc ttcaccagca agttcctcat
     1801 gaccaacagc tcctatgtgg cgcgcgccgg cggcgtgctg ctcgccgccg tcatgctgtg
     1861 ggacgtgatg ttccgcggcc agctcggcgt ctccgtctcc ttcctcgagg aactgtggtc
     1921 gcgcaacctc ggccatctct tcgtcagccc gttgcgccct tatgaatggg tgctgtcgct
     1981 gctgaccatg agcctcgtcc gcgtgatcat cggcgtcgtg ccggcggcgc ttctggcgat
     2041 cccgctctat cactactcga tcttcacgct gggcccgccg ctcatcgcct tcttcgtgct
     2101 gctgctgatc atgggctggg gcttgggcct catgatcggg gcgctgctgc tgcgccatgg
     2161 cctgggcgcc gagagcctcg cctggctcgc cgtcttcctg ctggcgccgg cgagcgccgt
     2221 ctattatccg gtctcggtgc tgccgccatg gctgcaatat ctggcatggg cgctgccctc
     2281 cgcccatgtc ttcgagggca tgcgcgcggt gatgttcgag ggcgtcttcc gcgtcgacca
     2341 tttcatggcg gcggcggcgc tcgatctcgt ctatctcttg ttcggcggcg caatcttcct
     2401 ttacgctttc cgccaggcgc gccagcgcgg cgcgctgctg caaatgggcg agtgaggaca
     2461 tggcgtttcc acggtcgacg cggcgccagg cgatcatcgg tggcgccggc ctgctcgcga
     2521 cggcggctgc cggcggcgcg atctacgccg cctggccgcg gggcccgcgt ctcgttgccg
     2581 ggcggcgcat gctcgaggtc gacggcaagg cggcggccgt gctcgggctc gagcaccggg
     2641 gcgcagcgct gtccctcgtc cagggcgggc gcttccaggt ggcgctcgag aaccgtctcg
     2701 acgaaccgac gctcgttcat tggcatgggc tcacgccgcc cagcgcgcag gacggcacgc
     2761 ccggcctgtc gcagcccgcg ctggcgcccg gcggccgcta cgactacgac ttcccgttgc
     2821 agcgggcggg caccttctgg atgcattccc atcagggctt gcagcgcgcg aagctgttgg
     2881 cggcgccgct catcatcgcc gatcccgccg atgccaggcg cgacgagcag gacgtggtga
     2941 tgttcctcgc ggatttttct ttccgcgctc ccgaggaact gctggcagag ctcacgcgcg
     3001 gccaggcggc caagcacgat atgcccggca tggatcatgg accgatggac catgcggagc
     3061 acgtggcggg gcaggacgat gctccggccg agcgcgtcca cgccaacgac atcgagttcg
     3121 acgcctatct cgccaatgat cgcaatttcg cggatcccga tatcgtgcgg gtcgagcccg
     3181 gcggccatgt caggctccgg gtcatcaacg gtgccgccgc caccaatttc tggatcgccg
     3241 tcgacggcaa tctcaggagc gagctgattg ccgtcgacgg caatccgatc gtgccgcgcg
     3301 agggccggcg gttcggcctc gccatggcgc aacgcatcga cctccggctg tctctgcccg
     3361 agggcgaagg cgcctatccc atctatgcgg tgcgcgagaa cgcggtacag cgcaccggca
     3421 tcatcctcgc cacggccaag ggcgaggtga agaagctggg cgccgctcag cgcttgccga
     3481 cgtcgcggct cgacgtcgcg ctcgaagggc tcctcgccgc ggccgagccg ctcgccgagc
     3541 gcgtcgtgga ccggacgctc agtgccgacc tcaccggcga catggcgagc ttcgtctggg
     3601 ggatcaacgg caagtcctat ggcgaggaca cgccgctcct cgtgcgcgcg ggcgagcggg
     3661 tcgagctcgt gatgcagaac cgcaccaaca tggcgcatcc gatgcatctc cacggccatc
     3721 gcttccaggt gacggcgatc ggcgaccggc gcttggccgg cgcggtgcgc gacacggtgc
     3781 tggtgccggt cggcaccagc gtcaccgtcg ccttcgatgc ggacaacccc gggaagtggg
     3841 ccttccactg ccacaacgag tatcacatgg ccgcggggat gatgacgtcg gtgcagtatg
     3901 agagctgacc gggagtcctg atctcagcgg ctcgcgagcg ccgagcgcga gaccgagagc
     3961 cgcgcttcat cgcctccttc tgcggaattt cgctgcctct ggcgtcattg gatcagcgcg
     4021 tgaagctggt cggccaattc atggccgggg agatcgggcg tgagcagctt ggcgaactgg
     4081 ccgtcggggg cgatgacata gatgagagag ttgtgatcga ccgtgtactc gccattgccg
     4141 agctgccgcg tgcggaaata gacatggtac tgattggcag tggccgcgat ctcctcgggc
     4201 gtgccggaaa ggccgacgat gcgcggatcg aaggatttga gatactgggt catggccgcc
     4261 cgcgtatcgt gctcgggatc gacggtgatg aagagcggct gcaccttgtc ggcgagcggg
     4321 ccgagcttgt cgagcgccac cccgatgttg ttgagggtga gcggacaggc atcggggcag
     4381 gaagtgtagc cgaaatagat gaccagccat ttgccgcggt agctctcatc ggtgacgctg
     4441 cggccgtcgg cggtggtcag cgcgaaatgg ccggcgatgt ccgccatggc cgcaggcggt
     4501 gcttcgctcg cggtgtgggc gagcgcgctc gggccgcgca gcgcggcacc gggtgccgtg
     4561 aggcagagca gcattgctgc aaagacggag cgccgccgga atgccgtgat catagctccc
     4621 tcatgccata aaaaggatcg cggcggcatg gttggggtcg ctggaccatg ccgccgctac
     4681 cgtcagtcgc gccgtggcgc gaccgctcct cgtggctgga cgtcgactgc gccgagcgtt
     4741 cggccgaccc ggaaaccgaa aaggttgacc cggttgtcga ggccgatcca gtcgcggagc
     4801 gcggcgcgga ggccctttgg agtgttgatc caggatccac cgcgcagaac acgctgatag
     4861 caatcgccgc tggtccaggc cgagccatcc gtcggcgcac cgttgtagct gtcgtggtag
     4921 cagtcctgcg tccactccca gacattgccc gccatgtcat agaggccgaa gccgttggcg
     4981 gcgaatgaac cgaccggcgc cgtttccttg ccgttccact ggccgccgca gccggtgcaa
     5041 ttcgcagtgt tctggacaac ggcgtt
//
LOCUS       DASYNS010000055         5426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4851301, whole genome shotgun sequence.
ACCESSION   DASYNS010000055 DASYNS010000000
VERSION     DASYNS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4851301"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VGP50_01715"
     CDS             <1..730
                     /locus_tag="VGP50_01715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8026113.1"
                     /translation="ISEAELAILPAYRGGAQETAVPWSRLVPRMLPTTFVYFCYAWTL
                     WLYLSWIPLYLQHAHHLDLKTSALFSALALWPGVIGDAMGGVVSDWVYKLTGNLATAR
                     RNVIVGSLLGSLICLAPIFFVTDVAVLTLCLGSAFFCLELTIGPIWSVPMDIAPRHAG
                     TASGIMNTGSAVAAIASPWLFGIIIDATAGWTLPFDNWDLPFAGSIALLLLGAVLAFR
                     MRPDRSIEGASVTPSLAAGTVSAE"
     gene            800..2011
                     /locus_tag="VGP50_01720"
     CDS             800..2011
                     /locus_tag="VGP50_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV8026114.1"
                     /translation="MTVRRGRHFLQIPGPSNVPDQILRAIGQPTIDHRGPEFSALTIG
                     AIAGLKRLFRCKGPVVILASSGTGAWEAALVNTLSPGDRVLACETGQFATLWHNMAKR
                     LGLEVDFIPSDWRHPIDPDEIEARLTADKAHAIKAVMAVQTETSTGVTSDIAAIRRAM
                     DRAKHPALLMVDAISSFAVTDLRQDEWRIDVTIAGAQKGLMLPPGLGFNALSAKAIEA
                     SKKAKLLRSYWDWQPLIQAGETGFFPFTPATTLLFGLKEAIAMLEEEGLENVFARHDR
                     LAEATRRAVAGWGLDVLCADPRAHSSTLTAVMMPPGHDADAFRRIVLERFDMSMGAGL
                     GKVAGKVFRIGHLGWLNELMLMGTLAGVEMGLAASGVPHRRGGVEAAMAYLTGNARAA
                     PAQAPAVAAHR"
     gene            2008..2778
                     /locus_tag="VGP50_01725"
     CDS             2008..2778
                     /locus_tag="VGP50_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV8026115.1"
                     /translation="MTEKTAALAAMLLAARRDGQPIADLPAELVPATAAEAYAVQDRV
                     TSALGPIAGWKVGAGSPPATPNCAPLLADLVAPSPARWPAARFRRRGVEAELAFRFAI
                     ALKPRARPYGEDEVWDAVASLHPAIEVVESRFALFPAFDPLALLADHQSNGAFCYGAA
                     AEDWRDVDFLSQPARLLIDGKEAARARGGNAAGHPRRLLAWLANHRARSGDGIADGDI
                     VTTGSHTGLVFAPPGATVTAEFDGIGSATLAFAASAGA"
     gene            2855..4480
                     /locus_tag="VGP50_01730"
     CDS             2855..4480
                     /locus_tag="VGP50_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026116.1"
                     /translation="MLSKASTLPPTGQTVAIVDDRISTLKILERLAASVGDRITVRSF
                     DRPARALEAARSSPPDLMIAAARMSDVDGAQFIRQLHALPGCGDVPVMVISAYEDHGL
                     REDALKAGASDVLLSPINHDEFRLRSRNLLMLRRHQQLLDERAATIEGPVAADERESR
                     DALRRSHDLLMRIIDAVPVLVSATDRDGRYVFVNDFYARQTGRPVAAIVGLTPCAVQD
                     SPEARAAQERDQRIIAGIDPPGAFEETIVEPGGPQRVLLTTKALLRDRDGEPSLVVTA
                     SLDITLRKEAELALVAAKEEAELASRSKTEFLANMSHELRTPLNAVIGFSQIMADETL
                     GSLGNPRYAGYARDIGASAHHLLGIISDILDVSKLEAGKVDLEESVVEFGQMLRDILY
                     LVSERARALAITVSVEFSEDLPRLRGDVLKLKQVLLNLITNAIKFSHKGGAVLIRGSL
                     AGDDLRIAVIDHGIGMDANEIATAVTRFGQVASTWSRRHSGTGLGLPLAIGIVELHGG
                     RIEIDSTKGVGTTVTVCLPASRLLRRDSRSKLI"
     gene            4597..>5426
                     /locus_tag="VGP50_01735"
     CDS             4597..>5426
                     /locus_tag="VGP50_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026117.1"
                     /translation="MAVRKAQGFVAALAVYAISLPLASPLLVSLALLPRPLVALWLLM
                     NLLGPATAAIVALRQGGAARGLMAGSGGEPAQIVIRVLVPGVILVYLLGLAATGVEHA
                     RLLPLIALDVAATLYAWMLLAHLLLQPGRSSLRRWVAMLSDVGLVSMFLHFGAALSVP
                     WFSLYFWIVLAFGVGPRALAAAAAATLMGFAAVYAATPYWQERPGLAAGVALALVLLP
                     AHMANLLRRLAPGTALSRSLASSGSAKAATRAAAHSGPLDILLAENDDPRQSLRAMLA
                     "
BASE COUNT          788 a         1913 c         1808 g          917 t
ORIGIN      
        1 catcagcgaa gccgagctcg ccattctacc ggcctatcgc ggcggtgcgc aggagacggc
       61 ggtgccgtgg tcgcgcctcg tgccgcgcat gctgccgacc accttcgtct atttctgcta
      121 tgcctggacc ttgtggctct atctgagctg gataccgctc tatctccagc acgcgcacca
      181 tctcgatctc aagacgtcgg cgctcttctc cgcgctggcg ctttggcccg gcgtcatcgg
      241 cgacgccatg ggaggcgtgg tcagcgactg ggtctacaag ctcaccggaa atctcgcgac
      301 ggcgcggcgc aatgtcatcg tcggcagcct gctcggttcc ctgatctgcc tcgcaccgat
      361 cttcttcgtg accgatgtcg cggtgctgac gctttgcctc ggctcggcct tcttctgcct
      421 cgagctcacc atcgggccca tctggtcggt gccgatggat atcgcgccgc ggcatgcggg
      481 gacggcgagc ggcatcatga acacggggtc ggcggtcgcc gcgatcgcct cgccctggct
      541 tttcggcatc atcatcgacg cgactgccgg ctggacgctg cccttcgaca attgggatct
      601 gcccttcgcc ggctcgatcg cgctgttgct gctgggcgcg gtcctcgcct tccggatgcg
      661 gcccgaccgg tcgatcgagg gggcgagcgt gacgccgtcg ttggccgcgg ggaccgtctc
      721 cgccgagtga acccgtcgcc cttgcgccga cgcctatttt ccgggcaaat tgccgccatt
      781 cccgcccgag gagagagcaa tgacggtgcg ccgaggcagg cattttttgc agattcccgg
      841 ccccagcaac gtgccggacc agatcctgcg ggcgatcggt caaccgacca tcgaccatcg
      901 cgggcccgaa ttttccgctt tgaccatcgg cgccatcgcc gggctgaagc ggctcttccg
      961 ctgcaaaggg ccggtcgtga tcctggcctc ctcgggcacc ggagcgtggg aagcagcgct
     1021 ggtgaacacc ctctccccgg gcgatcgcgt gctcgcctgc gagacgggac agttcgcgac
     1081 cttgtggcac aacatggcga agcgcctcgg tctcgaggtc gacttcatcc ccagcgattg
     1141 gcgccatccg atcgatcccg acgagatcga ggcccggctc accgcggaca aggcgcatgc
     1201 catcaaggcg gtgatggcgg tgcagaccga gacctcgacc ggcgtcacca gcgatatcgc
     1261 cgcgatccgc cgggcgatgg accgcgcgaa acacccggcg ctgctgatgg tcgatgccat
     1321 ctcctctttc gccgtgaccg atctgcgcca ggacgagtgg cgcatcgacg tgaccatcgc
     1381 cggggcgcag aagggactga tgctgccgcc gggcctcggc ttcaacgccc tctcggcgaa
     1441 ggcaatcgag gcgagcaaaa aggcgaagct gctgcggagc tattgggact ggcagccgct
     1501 gatccaggcc ggcgaaaccg gattctttcc cttcaccccc gcgaccacgc tcctcttcgg
     1561 cctcaaggaa gcgatcgcga tgctcgagga ggagggactc gagaacgtct tcgcgcgcca
     1621 tgaccgcctc gccgaggcga cgcggcgcgc cgtcgccggc tggggcctcg acgtgctgtg
     1681 cgccgatccg cgggcgcatt cgagcacgct cacggccgtc atgatgccgc ccggccacga
     1741 cgccgacgcc ttccgccgca tcgtgctcga gcgcttcgac atgtcgatgg gcgccggact
     1801 cggcaaggtc gcgggcaagg tgttccgcat cggccatctc ggctggctca acgagctcat
     1861 gctcatgggc acgctggcgg gagtcgagat gggattggcc gcttccggcg tgccgcaccg
     1921 ccgcggcggc gtcgaggcgg ccatggccta tctcacgggc aatgcccgcg ccgccccggc
     1981 gcaagccccg gccgtcgccg cccatcgatg acggaaaaga cggcggcgct cgccgcgatg
     2041 ctgctggcgg cgcgccgcga cggccagccg atcgccgacc tgccggcaga actcgtcccg
     2101 gcgaccgccg cggaagccta tgcggtccag gatcgggtga cgtccgcact tggccccatc
     2161 gccggctgga aggtgggtgc gggcagcccg ccggcgacac ccaattgcgc gccgctcctc
     2221 gccgatctcg tggcgccgag cccggcgcgc tggccggccg cgcgctttcg ccggcgcggc
     2281 gtcgaggccg agctcgcctt ccgcttcgcc atcgcgctca aaccgcgcgc gcgaccttac
     2341 ggcgaggacg aggtatggga tgccgtcgcg agcttgcatc cggcaatcga ggtggtggag
     2401 tcacgcttcg ccctgtttcc cgcgttcgat ccgctcgccc tcctcgccga ccaccagagc
     2461 aatggcgcgt tctgctacgg cgccgccgcc gaggattggc gcgacgtgga tttcctgagc
     2521 cagccggcgc gcctcttgat cgacggcaag gaagcggcgc gcgcgcgggg cggcaacgcc
     2581 gcgggtcatc cgcgccgtct cctcgcctgg ctcgccaatc atcgcgcgcg ctcgggcgac
     2641 ggcattgccg acggcgacat cgtcaccacc ggctcgcaca ccggactggt cttcgcgccg
     2701 cctggcgcga cggtcaccgc ggaattcgac ggaattggct cggcgacgct cgcctttgca
     2761 gcttctgcgg gagcataggc ctgcgacaat cgtttggtcc gtttatcagc agggacgact
     2821 tgcggcagcg cgccaccctg ccgatactga gcaggtgctc agcaaggctt cgactctacc
     2881 gccgacaggc cagacggttg cgatcgtcga tgaccggatc agcacgctga agatcctgga
     2941 gcgcctggcg gcctcggtcg gggacaggat caccgtgcgg agcttcgatc ggccggcgcg
     3001 cgcgctcgag gccgcgcggt cgagcccgcc cgatctcatg atcgccgcgg ccaggatgag
     3061 cgatgtcgat ggcgcccagt tcattcgcca gctgcacgcg cttcccggct gcggcgatgt
     3121 gccggtgatg gtgatcagcg cgtatgagga tcacggcttg cgcgaggatg cgctcaaggc
     3181 gggcgcaagc gacgtcctgc tgagcccgat caatcatgac gagtttcgcc tgcgctcgcg
     3241 caatcttttg atgctgcgcc gccaccagca gctgctggac gagcgcgcgg cgacaatcga
     3301 ggggccggtc gccgccgatg agcgcgagtc gcgcgacgcg ctgcgccgca gccacgacct
     3361 gctgatgcgc atcatcgacg ccgtaccggt gctggtgagc gccaccgacc gcgacggccg
     3421 ctatgtcttc gtcaacgact tctacgcacg gcaaaccggc cgtcccgtcg ccgccatcgt
     3481 cggcctcact ccctgcgcgg tgcaggacag cccggaggcg cgggccgcgc aagagcgcga
     3541 ccagcgcatc atcgccggca tcgatccgcc cggcgccttc gaagagacga tcgtcgagcc
     3601 cggcggcccg caacgcgtcc tgctgacgac gaaggcgctg ctgcgcgatc gcgacggcga
     3661 gccctcgctc gtggtcaccg cgtcgctcga catcacgttg cgcaaggagg ccgagctcgc
     3721 gctcgtcgca gccaaggagg aggcggagct ggcgagccgc agcaagaccg agttcctcgc
     3781 caatatgagc catgagctgc gcaccccgct caacgccgtg atcggcttct cccagatcat
     3841 ggccgacgag acgctggggt cgctgggcaa cccgcgttac gccggctatg cccgcgacat
     3901 cggggcgagc gcacatcatc tcctcggcat catcagcgac atcctcgacg tctcgaagct
     3961 cgaggccggc aaggtcgatc tcgaggaaag cgtggtcgag ttcggccaaa tgctgcgcga
     4021 cattctctac ctcgtcagcg agcgggcgcg cgccctcgcg atcaccgtct ccgtcgaatt
     4081 ctccgaggat ctgccgcgcc tcaggggcga cgtgctcaag ctgaagcaag tgctgctgaa
     4141 cctcatcacc aacgccatca agttcagcca caagggcggc gccgtcctca ttcgcggcag
     4201 cctcgccggc gacgacctgc gcatcgcggt gatcgaccac ggcatcggca tggacgcgaa
     4261 tgagatcgcc acggcggtga cgcgcttcgg ccaagtggcc agcacctgga gccggcgcca
     4321 ttccggcacc ggcctcggcc tgccgctggc catcggcatc gtcgagcttc atggcggtcg
     4381 gatcgagatc gacagcacca agggcgtcgg cacgaccgtc acggtctgcc tgccggccag
     4441 ccgcctgctg cggcgcgaca gccggtccaa gctcatctag atatagactt ttgcgccggt
     4501 gacgctcact atttggtgat gtgtcgatcg cgtgggagca tcgaccacaa gcacatgggt
     4561 ggggcgctgg ggacgaagga tagggctggc ggcggcttgg ctgtccgcaa ggcgcaagga
     4621 ttcgtcgctg ccctggcggt ctacgcgatc agccttccgc ttgcgtcacc gctgctcgtg
     4681 tcgctggcgc tattgccgcg cccgctggtt gcgctgtggc ttctgatgaa cctgctcggt
     4741 ccggccacgg cggcgatcgt cgctctgcgc caaggtggtg cggcgcgcgg cctcatggcc
     4801 ggaagcggcg gcgagccggc gcagatcgtc atccgcgtgc tcgtgcccgg cgtcatcctc
     4861 gtctatctgc tgggactggc cgcgacgggt gtggagcacg cccggctctt gccgctgatc
     4921 gcgctcgatg tcgccgcgac gctctacgcg tggatgctgc tcgcgcatct gctgctgcag
     4981 cccggacgct cgtccttgcg gcgttgggtc gcgatgctga gcgacgtcgg actcgtgtcg
     5041 atgttcctgc atttcggcgc cgcgctgtcg gtgccgtggt tctcgcttta tttctggatc
     5101 gtccttgctt tcggcgtcgg gccgcgcgcg ctcgcagcgg ctgcggcggc gacgctcatg
     5161 ggctttgccg cggtctatgc cgcgacaccc tattggcaag agcgccccgg gctcgcggca
     5221 ggcgtcgcgc tcgcgctcgt cctgctgccg gcgcatatgg cgaatttgct gcgccggctc
     5281 gccccgggca ccgcgctatc gcggagcctt gcttcgtccg gcagcgccaa agccgcgaca
     5341 cgcgccgccg cgcatagcgg cccgctcgac atcctgctcg ccgagaatga cgaccctcgg
     5401 caaagcttgc gcgccatgct cgccga
//
LOCUS       DASYNS010000056         7096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2043151, whole genome shotgun sequence.
ACCESSION   DASYNS010000056 DASYNS010000000
VERSION     DASYNS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043151"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VGP50_01740"
     CDS             complement(<1..1134)
                     /locus_tag="VGP50_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEV8026118.1"
                     /translation="MNEPVHPALLPLGLRDLLPPDAAIEAAIVARQMAVLASHGYERV
                     KPPLAEFEENLLSGAGAAMARETFRLMDPISQRMIGLRADMTPQVARIATTRLKNAPR
                     PLRLCYAGQVLRVKGSQLHPDRQVGQVGAELIGSDETSADVEVMALAADALAASGVGA
                     LSVDLGLPTLVPALCTALGLAPEAAEAVRRALDHKDAAAVAAVGGRAAALLGKLLDSA
                     GAAEKALKVLAALDLPAEAAGERTRLTKVVERLGQTVPDLHVTIDAVENRGFEYHTGV
                     SFTFFAKGVRGELGRGGRYRTGDGAGEPATGFTLYTDTVLRAIGRDTPSRRLYLPVGI
                     DPTLARRLRRDGWTTVAGLGPVADAEAEARRLGCGHRLEGEKIV"
     gene            complement(1183..2760)
                     /gene="serA"
                     /locus_tag="VGP50_01745"
     CDS             complement(1183..2760)
                     /gene="serA"
                     /locus_tag="VGP50_01745"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEV8026119.1"
                     /translation="MPRVLISDALSPRAVEIFAERGVETDVMTGLSPDELKKIIGKYD
                     GLAVRSATKVTKALLELADNLKVIGRAGIGVDNVDVPAATQRGIVVMNTPLGNSITTA
                     EHAIALMFALARQLPAADRSTQAGKWEKSRFMGVELYGKTLGVIGCGNIGSIVADRAL
                     GLKMKVVAYDPFLSAERAVALGVEKVELDDLLARADFITLHTPLTDATRNLLDAKALA
                     KTKKGVRIINCARGGLIVEEDLKAALDRGHVAGAALDVFPVEPAKQNVLFGRDDVIAT
                     PHLGAATTEAQENVALQIAEQMADYLTTGAVVNALNMPSLSAEEAGRLKPYMKLAEQL
                     GSFAGQMTTYEIKSVDIVYEGHVTELNVKPLTAIVLTALLSPQLDTVNMVNAPVICRE
                     RDIRVSETRVANPSNYHTLIRLTVTSERHARQLAGTLFGGDRPRIIEVEGVPMEAELG
                     AHMLFVRNKDKPGFIGNLGRTMGEAAINIATFHLGRTAAGGDAICLVQVDQALSDDVL
                     ECVRAIPNVLQARSLRF"
     gene            complement(2801..3958)
                     /locus_tag="VGP50_01750"
     CDS             complement(2801..3958)
                     /locus_tag="VGP50_01750"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEV8026120.1"
                     /translation="MKPVVRPQNPCFSSGPCAKRPGWSLNALEKALLGRSHRSKPGKA
                     KINDVVDRSRAVLGIPAEYRVAIVPASDTGAVEMALWSLLGPRGVDVLTWESFGEGWA
                     TDVTKQLKLTDVRLQRADYGQIPDLAEVDTDRDVVFTWNGTTSGVCVPNGDWIKPDRQ
                     GLTICDATSAAFAMALPWDKLDVITWSWQKVLGGEAAHGMLALSPRAVARLESYAPPR
                     PLPKLFRLTSKGKFSDGIFQAETINTPSMLVIEDAIDGLKWAEAVGGQKALMARAKSN
                     LAAVAEWVARTPWIDFLAADPAIRSWTSVCLKITDDWFQRLDIEAQRATVKRLEAMLD
                     AEGVAYDIAGHRDAPPGLRIWCGSTVERSNVEALLPWLDWAYGELRRAAAA"
     gene            complement(4134..4442)
                     /locus_tag="VGP50_01755"
     CDS             complement(4134..4442)
                     /locus_tag="VGP50_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026121.1"
                     /translation="MSIDFASDAVSIVAEDRGTDGARGKAHGIDAKGIQGPDQRVGIG
                     EVKLCEDEAGHRAVEEEIIPFDAGADGRCDNRAYQLLAMLEIGQPIGGDGCRGHEASP
                     "
     gene            4459..4644
                     /locus_tag="VGP50_01760"
     CDS             4459..4644
                     /locus_tag="VGP50_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026122.1"
                     /translation="MGTATFLVAFVPTYASIGICGAVILTVLRFVQGGGGGEWGGSAP
                     LSMEWAKTSEHRGFLAS"
     gene            4709..5482
                     /locus_tag="VGP50_01765"
     CDS             4709..5482
                     /locus_tag="VGP50_01765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8026123.1"
                     /translation="MNPERFLDWGWRIPFALSLVLVGIGLWIRLGIFETPTFQRLVAQ
                     HKIERTPTLEVIKRQPKEIILSALARMAEQAPFYIFTAFVFSYVSGTLKVPRDTVLTA
                     VLAASVLAFVAIPFFGYVSDRIGLKNMYLLGCVATGVFGFVYFALLNTRAGGLIFLAI
                     FLSLIPHNMMYGPQAALIAESFTGRLRYSGASLGYQLASLIAGGPAPLIATALFAKYA
                     TPYAIAWYILACAIISIIAVLALKDYTGKDISREYDEGG"
     gene            complement(5497..6561)
                     /locus_tag="VGP50_01770"
     CDS             complement(5497..6561)
                     /locus_tag="VGP50_01770"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEV8026124.1"
                     /translation="MAKTYDIAVIAGDGIGKEVVPEGMRVLEASARRHGFAFRWRDYD
                     WSCERFARTGAMMPKDGLEQLSSADGIFLGAVGYPGVPDHVSLWGLLIPIRRGFRQYV
                     NLRPVRLLAGVQSPLKDRKPGDIDFYVVRENNEGEYSEIGGRLYAGTPMEMAMQQTVF
                     TRVGCDRIMRFAFDLAMTRKKHVTSATKSNGIIHTMPYWDERFAAIAKDYPQARTDQY
                     HIDILTAHFVRNPHWFDVVVGSNLFGDILSDLGPAVVGSIGIAPSANINPEREFPSMF
                     EPVHGSAPDIAGKGVANPIGQIWSGAMMLAHLGETAAAAAVEAAIAGVLADASAPKTP
                     DLGGTATTKELGAAIVAALG"
     gene            complement(6660..>7096)
                     /locus_tag="VGP50_01775"
     CDS             complement(6660..>7096)
                     /locus_tag="VGP50_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026125.1"
                     /translation="RWLETRPPEQDAPRRRSTARPPLVDKLVHENAVEALEQHLGPDK
                     TAQMVDAAIKEIPERLERMLRQVSDRGRIAEDANELVAVAGNLGFNELRAQCRRLAEI
                     NGEADDWQVGVMVENAKTAAERAIAAMRRAERYVPSGVTEIG"
BASE COUNT         1146 a         2368 c         2341 g         1241 t
ORIGIN      
        1 tacgatcttc tcaccttcga gacgatggcc gcaaccgagc cgccgcgctt cagcctcggc
       61 gtcggcgacc ggcccaagcc cggccaccgt cgtccagcca tcgcgccgca accgtcgcgc
      121 cagggtggga tcgatgccga cgggaagata gagccgccgc gacggcgtat ccctgccgat
      181 cgcgcggagc acggtgtcgg tatagagcgt gaagccggtc gcgggctcgc cggcgccgtc
      241 accggtgcga tagcgcccgc cgcggccgag ttcaccgcga acgcctttgg cgaaaaacgt
      301 gaagctgacc ccggtgtgat actcgaaacc gcgattctcg acggcatcga tcgtgacgtg
      361 caggtcgggg accgtttggc ccagccgctc gacgaccttg gtgaggcgcg tccgctcgcc
      421 ggccgcttcc gccggcaggt cgagcgccgc cagaaccttg agcgccttct ccgccgcgcc
      481 ggcgctgtcg agcagcttgc ccagcagcgc ggcagcacgg ccgccgaccg cggccaccgc
      541 tgcggcatcc ttgtgatcga gcgcgcgccg aaccgcctcc gccgcttccg gcgcaaggcc
      601 gagcgccgtg cacagcgccg gcaccagagt cggcagaccg agatcgacgg agagcgcgcc
      661 gacaccgctc gccgccagcg cgtcggccgc cagcgccatc acctcgacat cggcgctggt
      721 ttcgtccgag ccgatgagct cggcgccgac ctgtcccacc tggcgatccg gatgaagctg
      781 actgcctttg acgcgcagca cctgccccgc atagcagagc cgcaacggcc ggggcgcgtt
      841 tttgagccgg gtcgtggcga ttcgggccac ctgcggcgtc atgtcggcgc gcagcccgat
      901 catgcgctgc gagatcggat ccatcaggcg aaatgtctcc cgggccatcg ccgcgccggc
      961 gccgctcagc aggttctcct cgaactcggc gagcggcggc ttgacgcgct catagccatg
     1021 gctcgccagc accgccatct gtcgtgcgac gatcgccgcc tcgatagcgg cgtcgggcgg
     1081 cagcagatcg cgcaggccga gcggcagaag cgccggatgc acaggctcgt tcatggccgc
     1141 ggaaatagcg gttttgccgg gccccggcaa cctccgacca ggctagaacc ggagcgagcg
     1201 cgcttgcagg acgttgggga tggcgcgcac gcattcgagc acgtcgtccg aaagcgcctg
     1261 gtccacctgc acgagacaga tggcgtcgcc gcccgcggcc gtgcggccca gatggaaggt
     1321 ggcgatgttg atcgccgctt cgcccatggt gcgcccgaga ttgccgatga agccgggttt
     1381 gtccttgttg cgcacgaaga gcatatgggc gccgagctcg gcctccatgg gcacaccctc
     1441 gacctcgatg atgcggggac ggtcgccgcc gaagagtgtt cccgcgagct ggcgcgcatg
     1501 ccgttcggag gtgacggtca ggcggatcag cgtgtgatag ttggacggat tggcgacgcg
     1561 cgtctcgctg acgcggatgt cgcgctcgcg gcagatgacg ggcgcgttca ccatattgac
     1621 ggtgtcgagc tggggcgaga gcagcgcggt cagcacgatg gccgtgagcg gcttcacatt
     1681 gagctcggtc acgtgacctt catagacgat gtcgaccgac ttgatctcat aggtggtcat
     1741 ctggccggcg aagctgccga gctgctcggc gagcttcatg tagggcttga gccggcccgc
     1801 ttcctccgcc gagagcgacg gcatgttgag cgcattcacc acggcgccgg tcgtcaggta
     1861 atccgccatc tgctcggcga tctgcagcgc cacattctcc tgcgcctcgg tggtggcggc
     1921 gcccagatgc ggcgtcgcga tcacgtcgtc gcggccgaag aggacattct gctttgccgg
     1981 ctcgaccggg aagacgtcga gcgctgcgcc cgccacatgg ccgcgatcga gcgccgcctt
     2041 gaggtcctcc tcgacgatga gcccgccgcg cgcgcagttg atgatccgca cgcctttctt
     2101 ggtcttcgcc agcgccttgg cgtcgagcag gttgcgcgtc gcgtccgtca agggcgtgtg
     2161 cagcgtgatg aaatcggcgc gcgccagcag atcgtcgagc tcgaccttct cgacgcccag
     2221 cgccaccgcg cgctcggcgg agaggaaggg gtcgtaggcg acgaccttca ttttgaggcc
     2281 gagcgcgcgg tcggcgacga tcgagccgat attgccgcaa ccgatgacac ccagcgtctt
     2341 gccatagagc tcgacgccca tgaagcgcga tttctcccat ttgccggcct gggtcgagcg
     2401 gtcggcggcg gggagctggc gggcgagcgc gaacatgagg gcgatggcgt gctcggcggt
     2461 ggtgatggaa ttgccgagcg gcgtgttcat cacgacgatg ccgcgctgcg tcgccgccgg
     2521 tacgtcgaca ttgtcgacgc cgatgccggc gcggccgatg accttcagat tgtcggcaag
     2581 ctccagcagc gccttcgtta ccttcgtggc ggagcgaacg gcgaggccgt catatttgcc
     2641 gatgatcttc ttgagctcgt cgggcgagag gcccgtcatg acatcggttt cgaccccgcg
     2701 ctcggcgaag atctcgaccg cgcgcggcga cagggcgtcg gaaatcaaga ctctgggcat
     2761 ggcttctatc cgcgagggaa taaggacgag gaggcgacgg tcaggccgcg gcggcgcggc
     2821 gcagctcgcc ataggcccag tcgagccagg gcagcagcgc ttcgacattg ctgcgctcga
     2881 cggtggagcc gcaccagatg cgcaaccccg gcggcgcatc gcgatgaccg gcgatgtcat
     2941 aggcgacgcc ctcggcatcg agcatcgctt cgagccgctt caccgtggcg cgctgcgcct
     3001 cgatatccag gcgctggaac cagtcatcgg tgatcttgag gcagaccgag gtccaggacc
     3061 ggatcgccgg atccgccgcc aggaaatcga tccagggcgt ccgagccacc cactccgcga
     3121 ccgccgcgag gttggatttg gcgcgcgcca tcaatgcctt ctggccgccg accgcctcgg
     3181 cccatttgag gccgtcgatc gcgtcctcga tcacgagcat ggagggtgtg ttgatggtct
     3241 cggcctggaa gatgccgtcg ctgaacttgc ccttcgaggt caggcggaag agcttcggca
     3301 gcggcctggg tggcgcgtag ctctcgagcc gcgccacggc ccgcgggctc agcgccagca
     3361 tgccatgggc agcctcgccg cccagcacct tctgccagga ccaggtgatg acgtcgagct
     3421 tgtcccaggg aagcgccatg gcgaaggcgg ccgaggtcgc gtcgcagatc gtgagaccct
     3481 ggcggtccgg cttgatccag tcgccgttcg gcacacagac gccggaggtg gtgccgttcc
     3541 aggtgaagac gacatcgcgg tcagtgtcga cttcggcgag gtccggaatc tgtccgtaat
     3601 cggcgcgctg taagcggacg tcggtcagct tgagctgctt ggtgacgtcg gttgcccagc
     3661 cctcgccgaa gctctcccag gtcaggacat cgacgccccg cgggccgagg agcgaccaga
     3721 gcgccatctc gacggccccg gtgtcggacg cgggcacgat ggcgacccgg tactctgcgg
     3781 gaatgccgag gacggcgcgc gagcggtcga cgacgtcgtt gatcttggcc ttgccgggct
     3841 tcgagcggtg cgagcggccg agcagcgcct tctcgagcgc gttgagcgac cagccgggtc
     3901 gcttggcaca aggtccagag gaaaaacagg ggttttgggg ccgaacaacc ggcttcatga
     3961 ctattccttc cagaataggt gcgacccgtt ggggggccgt gtcccgccgc ggacaatatg
     4021 cgcaatgctg caccgcgtca atcgccggac atgcggcatt tgatcttttc gccgggacga
     4081 ggtgcaaatc ccggagcttt gggctatctc tttgaagaga aagaacacgg gcctcaggga
     4141 gacgcttcat ggccacgaca accatcgccg ccgatcggct gtccgatctc gagcatcgca
     4201 agcagctgat acgcgcggtt atcgcagcga ccgtcggcac cagcatcgaa tggtatgatt
     4261 tcttcctcta cagcacggtg accggcctcg tcttcgcaaa gctttacttc cccaattccg
     4321 acccgttgat cgggaccctg aatgcctttg gcatctatgc cgtgggcttt gccgcgcgcc
     4381 ccgtcggtgc cgcgatcttc ggccactatg gagaccgcat cggacgcaaa atcgatactc
     4441 atcgccacgc tgctgctgat ggggactgcc acttttctcg tcgccttcgt cccgacctac
     4501 gccagcattg gaatttgcgg cgcggtcatt ctgaccgtgc tgcgcttcgt gcaaggcggc
     4561 ggcggcggcg aatggggcgg ctcggcgccg ctgtcgatgg aatgggcgaa gaccagcgag
     4621 catcgcggct tcctcgcctc ctagccgcaa ttcggcgtac cggccggtct ctgtcctcgc
     4681 caatctcgcc gtcctggcat tcagcaccat gaatccggaa cgctttctcg actggggctg
     4741 gcgcatcccc ttcgcgctga gcctggtcct cgtcggcatc gggctttgga tccgcctcgg
     4801 tatcttcgag acgccgacct tccagcgcct cgtcgcgcag cacaagattg agcggacgcc
     4861 gacgctcgaa gtgatcaagc ggcaacccaa ggaaatcatc ctttcggcgc tggcgcgaat
     4921 ggccgagcag gcgcccttct acattttcac ggctttcgtc ttcagctatg tcagcggcac
     4981 gctcaaggtg ccgcgcgaca ccgtgctcac cgctgtgctc gccgcgtcgg tccttgcttt
     5041 cgtggcgata ccgttcttcg gctacgtctc cgaccgtatc ggcctcaaga acatgtacct
     5101 gctgggttgc gtcgccacgg gcgtcttcgg cttcgtctat tttgcgctcc tcaacacccg
     5161 ggcgggcggg ctgattttcc tggcgatctt cttgtcgctc attccgcaca acatgatgta
     5221 cgggccgcag gcagcgctca tcgccgagag cttcacgggc cgcctccgct acagcggcgc
     5281 gtcgctcgga tatcagctcg cgtcgttgat cgccggcggc ccggcgccgc tcatcgcgac
     5341 agcgctcttc gcgaaatacg cgacgcctta cgcgatcgcc tggtacatcc tcgcctgcgc
     5401 gatcatcagc atcatcgcgg tgctcgcgct caaggactat accggcaagg acatcagccg
     5461 cgaatacgac gaaggcggct gaaacgccgg agcggctcag ccgagcgccg cgacgatcgc
     5521 ggcgcccagc tcctttgtcg tcgccgtgcc gccgaggtcg ggggtcttcg gcgccgacgc
     5581 atcggccagc acgcctgcga tcgcggcctc gacggcagcg gcggccgccg tttcgcccag
     5641 atgcgccagc atcatcgcgc ccgaccagat ctggccgatg gggttggcga cgcccttgcc
     5701 ggcgatgtcg ggggccgagc catgaacggg ctcgaacatc gagggaaatt cgcgctcggg
     5761 attgatgttg gcggatggcg cgatgccgat cgagccgacc acggcggggc cgagatcgga
     5821 gagaatgtcg ccgaagagat tggatcccac gaccacgtcg aaccaatgcg gattgcgcac
     5881 gaaatgcgcc gtcaggatgt cgatgtgata ctggtcggta cgggcctgcg ggtagtcctt
     5941 ggcgatggcg gcgaagcgct cgtcccaata gggcatggtg tgaatgatgc cgttcgactt
     6001 ggtggccgag gtgacgtgct tcttgcgcgt catggcgaga tcgaaggcaa agcgcatgat
     6061 ccggtcgcaa cccacgcgcg taaagaccgt ctgctgcatc gccatttcca tcggcgtccc
     6121 ggcgtagagc cgaccgccga tctcggaata ctcgccctcg ttgttctcgc gcacgacata
     6181 gaaatcgata tcgccgggct tgcggtcctt gagcggcgac tgcacaccgg caaggagtct
     6241 caccggccgc aggttgacat attggcggaa gccgcgccgg atcgggatca aaagccccca
     6301 cagggagacg tgatcgggaa cgccggggta gcccacggct ccgaggaaaa taccgtcggc
     6361 gctgctgagc tgctcgagcc cgtccttggg catcatcgcg cccgtcctgg caaagcgctc
     6421 gcagctccag tcgtagtcgc gccagcgaaa ggcgaagcca tggcgccgtg ctgacgcctc
     6481 gaggacgcgc atgccttcgg gcacgacttc cttgccgatg ccgtcaccgg cgatgacagc
     6541 gatgtcgtag gttttcgcca aggttctcct cccttcgctc gctggacgat agccgactgc
     6601 cgacggcccc gccacgcctt cgccacccaa tcctttcacg aggagagggg actgtcgcct
     6661 cagccgatct cggtcactcc cgagggcacg tatcgttccg cgcggcgcat ggccgcgatc
     6721 gcccgctcgg cggccgtctt ggcattctcc accatgacgc cgacctgcca gtcgtcggcc
     6781 tcgccgttga tctcggcaag gcggcggcat tgcgcgcgaa gttcattgaa cccgagatta
     6841 cctgcgaccg ccaccagctc attcgcatcc tctgcaatac gtccgcgatc tgacacctgc
     6901 cgcagcatgc gctccagccg ctcgggaatc tccttgatcg cggcatcgac catttgcgcc
     6961 gtcttgtcgg gaccgagatg ttgctcgagc gcctcgaccg cattctcgtg gacaagcttg
     7021 tcgacgagag gcggccgcgc cgtgctgcgg cgccgcggcg catcctgctc ggggggccgg
     7081 gtctcgagcc agcggt
//
LOCUS       DASYNS010000057         3579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3291011, whole genome shotgun sequence.
ACCESSION   DASYNS010000057 DASYNS010000000
VERSION     DASYNS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3291011"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VGP50_01780"
     CDS             complement(<1..1496)
                     /locus_tag="VGP50_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV8026126.1"
                     /translation="MTSEISLTPSAAHLRHGSNIGQPLTRRDGVLKVTGRARYAADNH
                     PPGMLYAVLAVSSIARGRVTFLDVLAAKAHSGVVDVMTPANKPPLAEDPDAKTNPFMF
                     RLDLLQNDRVRYANQPIAVVIARTLEAATEGAALLSPHYEKLPARIGLDGDESFVPPT
                     VGVGNPANLQRGDVEAALAAALYRVDATYETPAQYHNPLEPHAIVAAWNGDALSIDTP
                     SQGLAMAQARIAGLFGIAPEKVHIRSPFLGGGFGCKGLISGPQILGIMAARLVGKPVK
                     LVLRREQMYGPVGHRAPTRQTLRIAADGDGKLTAIAHHAKTTSSSFDDFFEPAADASH
                     TLYASRAIATSHEVVRVDTGTPMFMRAPGEATGSIALESAIDEAAEACGMDPLAFRLK
                     NYAEVEPISGKPFSSKALRACYARGVERFGWSPRPLAPRQMRDDAGFLVGYGMGTATF
                     PALMFEAEARAVVRSDGSGLMETGAHDMGQGASTALAQIAADGLGLEL"
     gene            complement(1486..2544)
                     /locus_tag="VGP50_01785"
     CDS             complement(1486..2544)
                     /locus_tag="VGP50_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEV8026127.1"
                     /translation="MNTFDYVRPATIADAIAAAAAPGAAYLAAGTNMLDLMKGGIAGP
                     SRLVDVTHLPGLDRIERLADGSTRIGALVRNADLAHDRDFARSYPAIAEALLSGASPQ
                     LRNAATVGGNLLQRTRCGYFYDVASACNKRAPGAGCDALHGENRLHAVLGWSESCIAT
                     HPSDFCVPLVALDALVEIEGRSGRREIALEAFHRLPGDTPERESALEPGDLIVALRLP
                     GEAASFAAHARYLKVRERTSYAFAVVSAAAALRIEKGTIREARLALGGVAAKPWRARA
                     AEEILADSRPGYAAFSRAAAAALAEAKPSGDNRFKIELAQRILMRALALAAAGTPERV
                     PALPASPFSSVPRALHDI"
     gene            complement(2541..3038)
                     /locus_tag="VGP50_01790"
     CDS             complement(2541..3038)
                     /locus_tag="VGP50_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV8026128.1"
                     /translation="MTLSISLTVNGVRRDVALGDPRVTLLDLLRERLDLTGTKKGCDR
                     GQCGACTVLVDGRRINSCLALAVSHDGADILTIEGVARGDALHPVQAAFIAHDGFQCG
                     FCTPGQIMSAIGLIQEGQAGDDPERVREGMSGNICRCGAYAGITDALLDAQKNLAATE
                     ERRSA"
     gene            3181..>3579
                     /locus_tag="VGP50_01795"
     CDS             3181..>3579
                     /locus_tag="VGP50_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8026129.1"
                     /translation="MGDPSAETARKPRADALRNRERVLEAAKAVFSAGGPDASLEAVA
                     RRAAVGIGTLYRHFPTREALFEAVYRREIEQLGELAEQLKGEAEPVEALRRWMRSNVE
                     VVATKKGMLAALAPAMDGSSELYAYSFDRLT"
BASE COUNT          575 a         1153 c         1270 g          581 t
ORIGIN      
        1 tcgagctcga gccccagccc gtcggcggca atctgggcga gcgcggtcga cgcaccctga
       61 cccatgtcgt gggccccggt ctccatcagg ccggacccgt cgcttcgcac gaccgcacgc
      121 gcctcggcct cgaacatcag cgccggaaat gttgcggtgc ccatgccata gccgacaagg
      181 aagccggcgt cgtcacgcat ctgccgcggc gcgagcgggc gtggcgacca gccgaaacgc
      241 tccactcccc gcgcgtagca cgcgcgtaaa gccttcgacg agaacggctt gccggagatc
      301 ggctcgacct cggcatagtt cttcagacgg aaggccagcg gatccatccc gcaggcctcg
      361 gcggcttcgt cgatcgcgct ttcgagcgcg atcgatccgg tcgcctctcc cggagcgcgc
      421 atgaacatcg gcgtgccggt gtcgacccgg accacttcat gcgaggtggc gatggcgcgg
      481 ctcgcgtaaa gggtatgcga ggcgtcggcg gccggctcga agaaatcgtc gaagctgctc
      541 gacgtcgttt tcgcgtgatg agcgatggcg gtcagcttgc cgtcgccatc ggcggcgatg
      601 cggagcgtct ggcgcgtggg cgcgcgatgg ccgacaggcc cgtacatttg ctcacggcgc
      661 agcacgagct tgaccggctt tcccaccagc cgggccgcca tgatgccgag gatctgcggc
      721 ccggagatca ggcccttgca gccgaagccg ccgccgagga acggactgcg gatatgcact
      781 ttttccggcg cgatgccgaa gagcccggcg atccgcgcct gcgccatggc gaggccctgg
      841 ctcggcgtgt cgatggagag cgcatcgccg ttccatgccg cgacgatcgc gtggggctcc
      901 agggggttgt gatactgggc gggtgtctcg taggtcgcat cgacgcggta caacgcggca
      961 gccagcgccg cctccacatc gccgcgctgc aggtttgccg gattgccgac gccgacggtg
     1021 ggcgggacga agctttcgtc gccgtcgaga ccgatgcggg ccggcagctt ttcgtagtgt
     1081 ggcgagagaa gcgccgctcc ttcggtcgct gcctcgagcg ttcgggcgat caccactgcg
     1141 atcggctggt tcgcatagcg cacgcgatcg ttctgcagaa gatcgaggcg aaacatgaag
     1201 gggttcgttt tcgcgtcggg atcttccgcg agcggcggct tgttggcagg ggtcatgacg
     1261 tcgacgacgc cggaatgggc cttggccgcc agcacgtcga ggaaagtcac gcggccgcgc
     1321 gcgatgctgc tgaccgccag aactgcgtag agcatcccgg gcggatgatt gtccgcggca
     1381 tagcgggctc tgcccgtgac cttgagaacg ccgtcgcggc gggtcagcgg ctgaccgata
     1441 ttcgagccgt gccggagatg ggcggcagac ggggtgaggc tgatctcaga tgtcatggag
     1501 ggctctggga acggaggaga aaggggaggc gggcagggcc ggaacgcgct ctggcgttcc
     1561 cgccgcggcg agagccaggg cgcgcatcag gatccgctgc gcgagctcga tcttgaatcg
     1621 attgtcgccg gagggcttgg cctcggcgag cgccgccgcc gctgcgcggc tgaaggcggc
     1681 gtaacccgga cgcgagtccg cgaggatctc ctccgccgca cgcgctcgcc acggcttggc
     1741 agcgacgccg ccaagggcca gccgcgcttc gcggatggtg cccttttcga tgcgcagcgc
     1801 cgcggcagcg gacacgacgg cgaaggcata agaggtgcgt tcgcggacct tcagatagcg
     1861 ggcatgcgcc gcgaagcttg cggcctcgcc cggcagccgc agggcgacga tcagatcacc
     1921 cggctcgaga gcgctttcgc gttcgggcgt atcgccgggc aaacggtgaa aggcctcgag
     1981 cgcaatctcg cgccgcccgg acctgccttc gatctcgacg agcgcgtcga gtgccacgag
     2041 cggtacgcag aaatccgacg gatgggtcgc gatgcaggac tcgctccagc ccagtacggc
     2101 gtgcaatcga ttctcgccat gaagggcgtc gcatcccgcg cccggcgcgc gcttgttgca
     2161 ggcgctggcg acgtcgtaga aatagccgca gcgtgtccgc tgcaacagat tgccgccgac
     2221 cgtcgcggcg ttgcgaagtt gcggcgaggc gccggacaag agcgcctccg ctatagcggg
     2281 atatgagcgg gcgaaatccc ggtcatgcgc gagatcggca ttgcgaacga gggcgccgat
     2341 ccgcgtgctg ccgtccgcca ggcgttcgat gcggtcgagc cccggcaggt gcgtgacgtc
     2401 gacgagacgg ctcggaccgg caatgccacc cttcatcagg tcgagcatgt tcgtgccggc
     2461 cgcgaggtat gcggcacccg gcgcggcggc ggcggcgatg gcatcggcga tcgtcgccgg
     2521 ccggacatag tcgaaggtgt tcatgcggag cgcctctcct cggtcgcggc gaggtttttc
     2581 tgcgcgtcca gcagggcgtc cgtgatcccc gcatacgcgc cgcatcggca gatgtttccg
     2641 ctcatgcctt cgcgcacgcg ctccgggtcg tcacctgcct gtccttcctg gatcaggccg
     2701 atggcgctca taatctgacc gggcgtgcag aagccgcatt ggaagccatc atgggcgatg
     2761 aacgcggcct ggacggggtg gagcgcgtcg ccgcgggcga cgccctcgat cgtgaggatg
     2821 tcggcgccat cgtggctgac cgccagagcg aggcaggaat tgatgcgtcg tccgtcgacg
     2881 agaacggtgc aggcgccgca ctggccgcgg tcgcatcctt tcttggttcc cgtcaggtcg
     2941 agacgctcac gcaggagatc gagcagcgtg acgcgcgggt ctcccaacgc gacatcccgc
     3001 ctgacgccgt tcacggtgag gctgatggag agagtcatcc attgctccga agagtgctat
     3061 ggtgaaattg gatcgcggcg gtcatgcacg ccgcgcggct tcaagatccg tacccgtcgc
     3121 cccacatata cggaggcacc ctccgctttt caaggtgaca aggacatcga gaccagtgct
     3181 atgggagatc cgtctgcaga aaccgcccgg aagccccgcg ccgatgcgtt gcgcaatcgt
     3241 gagcgcgtcc tcgaggcggc gaaggccgtc ttcagcgccg gcggcccgga tgccagtctc
     3301 gaagccgtcg cgagacgcgc cgcggtcggt atcggcacgc tctaccgcca tttcccgacg
     3361 cgcgaagcgt tgttcgaggc cgtctatcgt cgcgagatcg agcagctcgg cgagcttgcg
     3421 gaacagttaa agggcgaagc tgagccggtc gaggccttgc gccgctggat gcggtccaat
     3481 gtcgaggtcg tcgcgaccaa gaaggggatg ctggcggcgc tggcgccggc gatggacggc
     3541 tcgtcggagc tctacgctta ttccttcgat cggctgacg
//
LOCUS       DASYNS010000058        11655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3603132, whole genome shotgun sequence.
ACCESSION   DASYNS010000058 DASYNS010000000
VERSION     DASYNS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3603132"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1157)
                     /locus_tag="VGP50_01800"
     CDS             complement(186..1157)
                     /locus_tag="VGP50_01800"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV8026130.1"
                     /translation="MNAENASIHGPALRLGLRDWAMIRAIAETGGVTPAARRLNLSQS
                     ALSHRLLDLEARLGVRLFDRVGKTMRSTPVAEALATSAAAVLALCREAEQAIEIATRQ
                     SDRRPLRAAASCFSYYAWLAETLAEFGLQHRHLDVSVYLQPARDEFAVLDRGLADVII
                     TAHPPRRSDLETMPVFAQEVVALAPPDHPLTRRAEGGGAVRWTDLRDEMLLIHDLPST
                     DEASLRKAVWGRAAGGDRVRRVPLTEAMAALVKSGFGVAVVNRPPGRSPFDGQGLATI
                     ALAPRHDRGFWAVWRRADAARLPLRDLAETIARRRGSSAAAARPSGS"
     gene            1239..1634
                     /locus_tag="VGP50_01805"
     CDS             1239..1634
                     /locus_tag="VGP50_01805"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV8026131.1"
                     /translation="MASLYKEIVINTAPETVWAAIRDFGAVRAIVPGLVDCRLEDDVR
                     IVTFPDGRVAREHLVGIDDDRRRLVYAEPGGRFVTRSASMQVFADGDNGARLVWINDV
                     LPDTFAGLIGTNMNRGLIAIKRSLEESNP"
     gene            complement(1673..1885)
                     /locus_tag="VGP50_01810"
     CDS             complement(1673..1885)
                     /locus_tag="VGP50_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026132.1"
                     /translation="MSGAGYREAKNETPTGVAVFPWDFRSVRSLAERANNVVHWTEMP
                     RGGHFAAVDAPDLLVGDVQSFFAKVR"
     gene            complement(1940..3046)
                     /locus_tag="VGP50_01815"
     CDS             complement(1940..3046)
                     /locus_tag="VGP50_01815"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002938.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEV8026133.1"
                     /translation="MARPSPRPPPVTIATWLVFTSDSIDRSPFQGWPQAFNSVLRHAR
                     KPEQRRLPDRWRGPAERNLGLRSVHMTVEIGARPFGPKKYLQVKGCRMAYIDEGEGAA
                     IVFQHGNPTSSYLWRNVMPHLRGMGRLVACDLIGMGDSAKLPPAGPDRYHYEEQRDYL
                     FALWDALGLGERVVLVLHDWGSALGFEWARQNPGRVQGIAYMEAIVAPLLWSDYPEGP
                     RRSAFRAFRSPEGEPQVLERNAFIERVLPGGIKRQLTDTEMTEYRRPFLQPGEDRRPT
                     LSWPRDLPIAGEPQTVARIVQAYSAFLAGSQLPKLFINADPGALLTGRLRDLCRTWPN
                     QSEITVSGLHFVQEDSPHEIGEAVADFVDRVRRT"
     gene            2976..3803
                     /locus_tag="VGP50_01820"
     CDS             2976..3803
                     /locus_tag="VGP50_01820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8026134.1"
                     /translation="MESDVKTSHVAIVTGGGRGLGRAMVLGVALAGIHVVATAARERA
                     EIEAVAEEVRQRCGEARVLALVTDVTQEDDCAKAVDAALKRFGRLDILVNNAGRGMKY
                     VSNEFLTEPTRFWDVAPETWRLVIDTNVNGPFMMARHAVPVMLKAGWGRIVNVSVGQG
                     TMRRRGFSPYGPSKAALESETIIWAQDLEGTGVTVNALLPGGATLTGMVPQTVSENVK
                     SAMLDPSIMVPPLLWLVSPDADGMTGRRLVATKWLAGSDGKSAAEAATEQAGWSNGV"
     gene            3978..4979
                     /locus_tag="VGP50_01825"
     CDS             3978..4979
                     /locus_tag="VGP50_01825"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8026135.1"
                     /translation="MARPCTRRRLIKAGAALIGGVASPAIGFPTILRAATVTRLKCVT
                     LATGVSVIVDAYMQAKRFDLKNDVDLVAVNSYTSIANYYNDFAAGTFDVAIGSWDTFA
                     QMYGRGIPIRLACTAASAELVSIVAAKNGPATIEELRGKTLAAVQASGSYKLSKMVIQ
                     DIWGIALEKDVPVENVPNPAQALTMIMADRAAAALSWEPSVSGAIARVPDMMPIFNLG
                     AAYRQHRHGALPYFTYAIQSAVLKGRPDLAARVAKSFTDCIAEIMADPSEAIALAAPE
                     MKIAPQALRIGFDSGRLQLKAEPMSNPATRRAVARAADYMHRNGGLADSVDDGFFAL"
     gene            5026..5808
                     /locus_tag="VGP50_01830"
     CDS             5026..5808
                     /locus_tag="VGP50_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV8026136.1"
                     /translation="MTSAADSPAVSRQSDLLAGAIVVLAIVVVMQIASLVVPPYVVPA
                     PLAVATALIDDLRQDWLHVAITLARLGAAVLAAMTIGTAVGVVMGMSPRLRPYLRALV
                     VIDTGIPALSWMLVAVFWFRGPELRIFFIMAVILVPFYALTVHDGIRALPKDWLEMCE
                     SFRPSRAQVLRLLILPHILPYVLTTTRSMIGYATRMLIFAEVIGSALGIGARMGLAQA
                     TFHIEDVFAWTVLLVVLNLVLQAAITATESRLLRWRPAIEVR"
     gene            5805..6578
                     /locus_tag="VGP50_01835"
     CDS             5805..6578
                     /locus_tag="VGP50_01835"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8026137.1"
                     /translation="MTAPPPSTIEISNVTHRFNGQLAVDDLSLAVAAGETVALVGRTG
                     AGKSTALHLVMGTIAPHAGTVRVAGVDPCRDVAALRGKLAISFQNDRLLPWRTAVENV
                     AFGLEILGHARSDCRRRARDWLARVKMAGAEEKYAHELSGGMRQRVSLARALAVDPAI
                     LLLDESFSQLDHVTSQELRRDVSRLIHALGKTCLFVTHRIDDAIEMADRVVVVRAPAR
                     VALEVRPSDAVRGNPERAARLHDDIAAALGGDERTLEST"
     gene            complement(6660..8354)
                     /locus_tag="VGP50_01840"
     CDS             complement(6660..8354)
                     /locus_tag="VGP50_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV8026138.1"
                     /translation="MFILDRLRIKAKLGVLVGVSVVALALAIVLAASLQYQRMIDDRI
                     AKLRGIIEIAEGVARALEDEVIAGKTTREEAMARFRTYAHSMWYDDHHDYLLALTMDG
                     YFYIYTPDLKQEDTDRRQGKDVTGKPLYGSMAEIARTTKEGALTYYYKKPGEDTPMPK
                     LTFVRLYQPWNVILATGVWIDDIEAEYRAVLLELGGAATALIAMVCLLAYLISRNIAA
                     PLGSLKRKMEKLAAGHLATAVEEVERRDEFGEMARAVRVFKDNAIAMREMRAEQDELK
                     RRAEEEKRKALAAMADTFEARVRGIVGTVFRATGAMQGVARSMAETVETTRRQALAVA
                     TGADQATTNVQTVAAASEELSESIGEIGRQVTQAATVARKAATEGQHTNDSVAGLAEA
                     AQKVGEVVALINDIASQTNLLALNATIEAARAGDAGKGFAVVAAEVKSLATQTAKATD
                     DIRAQIGAIQQETTSAVAAIKGISQTILNVNTISSAIAAAVEEQMAATREINRNVQEA
                     AGGTREVSRNIAGVSAAVDIAGKAASEVRAAADELAREAAALNHEVDQFLTTVRAA"
     gene            8353..8490
                     /locus_tag="VGP50_01845"
     CDS             8353..8490
                     /locus_tag="VGP50_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026139.1"
                     /translation="MAVSFERAGKIRRQAYVGANLPAAGELLVKHVIREMTAERDALS
                     A"
     gene            complement(8686..9975)
                     /locus_tag="VGP50_01850"
     CDS             complement(8686..9975)
                     /locus_tag="VGP50_01850"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV8026140.1"
                     /translation="MLAAADNDMLTLTGPGTPMGQFFRRFWQPFALSRELPKPDSPPI
                     RVRLMGEDLVAFRDSRGRIGLLEPRCPHRGANLFYGRNEECGLRCVYHGWKFDVTGKA
                     VELPNVPPDSRYHETIAVKAYPTREFGDIVWAYLGPPGRVPEVPQLEFGVVPAEQRFV
                     TKKLQQCNWAQAVEGALDTCHFSFLHMPAPGVASNRNPAVAADETRMRWVRNDPMPRF
                     SIVEHPVGFVAGGARKADGEDLYWRTTQFMLPAHATTPSTLPGENYFGYTFVPITDDS
                     CWIYTYAWNPERPLSDAERAKFAAGHSVLAEVGPDYVPIRNRDNDYMIDRDEQKHRSF
                     TGVRGVAEQDAMIQESQGGIADRTREHLTPTDVAIVRFRRLLLAGAKALIEGREPEAP
                     WRDAAYRLRSGSWVAAAGVPFETVMKERFGDPIGAVV"
     gene            10309..10635
                     /locus_tag="VGP50_01855"
     CDS             10309..10635
                     /locus_tag="VGP50_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV8026141.1"
                     /translation="MYRLLPAMTILTAIIAAPAIAEDSGVTITIKDHRFVPDMVEIPA
                     GTKVKIVVRNEDTTTSEFESVAFHREKVVQPGSEITLFVGPLDPGSYEFFDDFHPETR
                     GHLVAK"
     gene            10647..11486
                     /locus_tag="VGP50_01860"
     CDS             10647..11486
                     /locus_tag="VGP50_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEV8026142.1"
                     /translation="MLATLIIVFREVLEAALIVGIVLAASRGVPRREQWISAGIAGGV
                     LGALIVASLASEIASAVEGVGQELFNAGILFAAVGMLGWHNIWMQRHGRELAQSATAI
                     GRAVLAGTRPLYALAVVVGVAVLREGSEIVLFLYSIAVAGGSSATSMITGGVLGLAVG
                     VGVGAGLYLGLLAIPLRHLFTVTSWLILFLAAGMASQGAAFLLQADLLPALGNNLWDT
                     SFLLSERSLPGQVVHVLLGYSAQPAGIQVVFYLATLVIIGSLMRLLGGMPAPAPRRDL
                     ARS"
BASE COUNT         1909 a         3826 c         3869 g         2051 t
ORIGIN      
        1 cgtcaacgcc tcctaccagc ttggcgaatt cgagagcggc atgaccgcgg ccttgttcgg
       61 tgcgatgccg gcagccgcgc tgggcgggct cgggaccatc gccgtggcgc tcttatggat
      121 gaaattgttt ccaacgctgc gggacgtcga gcgactggaa taagcagaaa gcggatcagg
      181 cggcttcagg atccggacgg gcgcgcggcc gcggccgaag agccgcgccg ccgagcgatg
      241 gtctcggcga ggtcgcgcaa gggcaggcgc gcggcgtcgg cgcgccgcca caccgcccag
      301 aacccacggt cgtggcgcgg ggcgagcgcg atcgtcgcta ggccctgacc gtcgaagggc
      361 gaccggcctg gcgggcgatt gaccacggcg acgccgaatc ccgatttcac cagcgccgcc
      421 atggcttcgg tcaacggcac ccggcgaaca cgatcgccgc ccgccgcccg tccccagacc
      481 gctttgcgca gactcgcctc atccgtcgat ggcagatcgt ggatcagcag catctcatcg
      541 cgcagatcgg tccagcgcac cgcaccaccg ccttccgccc gccgcgtgag cgggtgatcg
      601 ggcggcgcca gcgccaccac ctcctgcgca aacacgggca tggtctcgag gtcgcttcgc
      661 cgcggcggat gggcggtgat gatgacgtcc gcgaggcccc ggtcgagcac ggcgaactcg
      721 tcccgggccg gctgcagata gacgctcacg tcgagatggc ggtgttgcag gccgaactcg
      781 gccagggtct cggccagcca ggcgtaatag ctgaagcagc tggcggcggc ccggagcggc
      841 cggcgatcgg attgacgggt cgcgatctcg atggcctgtt cggcttcgcg gcacagggcc
      901 agcaccgccg ccgccgatgt tgccagcgct tcggcaaccg gcgtcgatcg cattgtcttg
      961 ccgacccggt cgaagagacg cacgccgagg cgcgcctcca ggtcgagcag ccgatggctc
     1021 agcgccgatt gcgacaggtt gagccgccgc gccgccgggg tgacgccgcc ggtctcggca
     1081 atcgccctga tcatcgccca gtcgcgcagg cccagccgca gcgcaggacc atggatcgat
     1141 gcgttttccg cattcatatg acgcacacta tgcgcttttt ctctcgaccc gtgaagctcc
     1201 atgatggccc gcgacatcgg cccacccggg agatcgacat ggcgtctctc tacaaagaga
     1261 tcgtgatcaa caccgctccc gagacggtgt gggcggcgat tcgcgacttc ggcgcggtgc
     1321 gcgcgatcgt gcccggcctg gtggattgtc gcctcgagga cgacgtccgc atcgtcacct
     1381 tccccgacgg ccgcgtggcg cgcgagcatc tggtcggcat cgacgatgat cgccgccggc
     1441 tggtctatgc cgagccgggc ggccgtttcg tcacccgcag cgcctcgatg caggtgttcg
     1501 ccgatggcga caacggcgcc cgcctggtct ggatcaacga cgtcttgccc gacaccttcg
     1561 ccggtctgat cgggacgaat atgaaccgag gcctcatcgc cataaagcgc tcgctcgagg
     1621 agtccaaccc gtagagcggt gctgccgcgt cccgcgattg gcggcgcgac ccctacctga
     1681 ccttggcaaa aaaactctgc acatctccga ccagcagatc cggcgcgtcg accgccgcaa
     1741 aatgcccgcc gcgcggcatt tcggtccagt ggacgacgtt gttggcgcgc tccgcaaggc
     1801 tcctgaccga gcgaaaatcc cacgggaaca cggcgacgcc ggtcggcgtc tcgttcttcg
     1861 cctcgcgata gccggcgccg ctcactgggg atcgacgacc cctcttagac gaatcgacag
     1921 cgttagagcg cggcgccgcc taggtccggc gcactcgatc gacgaaatcg gcgaccgcct
     1981 cgccgatctc gtgcgggctg tcctcctgca cgaagtgcaa tcccgagacg gtgatctcgc
     2041 tctgattggg ccaggtccgg caaaggtcgc gcagccgccc ggtcaggagc gcccccgggt
     2101 ccgcattgat gaagagcttg ggaagctggc tccccgccag gaacgcgcta taggcctgga
     2161 caatgcgggc cactgtctgg ggttcaccgg cgatggggag atcgcgaggc cagctgaggg
     2221 tcgggcgacg atcttcgccc ggctggagga aggggcggcg atattcggtc atttcagtgt
     2281 cggtgagctg gcgcttgatc ccacccggca gcacgcgctc gatgaacgca ttgcgctcca
     2341 gcacctgcgg ctcgccctcc ggcgaccgga aggcccggaa ggcgctgcgc cgggggcctt
     2401 ccggatagtc ggaccaaagg agcggcgcga cgatagcctc catgtaggcg atcccttgca
     2461 cgcgaccggg gttctgcctg gcccactcga agccgagagc cgacccccaa tcgtggagga
     2521 ccagcacgac ccgctcgccg aggccgagcg cgtcccacag ggcgaagagg tagtcgcgct
     2581 gctcttcata gtgatagcga tcggggccgg ccggcgggag ttttgcggaa tcgcccatgc
     2641 cgataaggtc gcaagcgaca agccggccca tcccacgcag atgaggcatc acgttgcgcc
     2701 agaggtagga ggatgtcgga ttgccgtgct gaaagacgat ggcggcgcct tcgccctcgt
     2761 cgatgtaagc catcctgcat cccttcacct gcagatactt tttgggaccg aatggccgcg
     2821 cgccgatttc aacagtcatg tggacactcc tcaggccgag gtttcgttct gccggtcccc
     2881 gccatcgatc aggcaagcgc cgctgttctg gttttcgcgc atgcctcaga acactgttga
     2941 aggcctgcgg ccagccctgg aagggagatc gatcgatgga atccgatgtg aagacgagcc
     3001 acgttgcaat tgtgacaggc ggaggccgcg ggctcggccg ggccatggtc ctcggagtgg
     3061 cgctagcggg aatccatgtc gtcgcgacgg cggcgcgcga acgcgccgag atcgaagccg
     3121 tcgcagaaga ggttcggcag agatgcggcg aagctcgggt gctcgcgctt gtcaccgatg
     3181 tcacgcagga agacgactgc gccaaggctg tcgacgccgc gctcaagcgg ttcgggcgac
     3241 tcgatatcct ggtgaacaat gccggtcgcg gcatgaaata cgtcagcaat gagttcctga
     3301 ctgaaccgac gcgcttctgg gacgtcgccc cggaaacgtg gcgcctggtg atcgacacca
     3361 acgtgaacgg tcctttcatg atggcaagac acgcggttcc cgtgatgctg aaagcgggct
     3421 ggggacggat cgtgaatgtt tcggtgggcc aagggaccat gcgccgacgc ggcttttctc
     3481 cctatggtcc ttcgaaggcg gcgctcgaat ccgagacgat catctgggcg caggatctcg
     3541 agggcacggg tgttaccgtg aacgcgctgc tgccgggagg agccactctt accggcatgg
     3601 ttccgcagac cgtgtcggag aacgtgaaat cagcgatgct cgatccctcg atcatggtgc
     3661 cgccgctgct ttggctcgtg tcgccggacg ctgatgggat gaccggccgt cgtctggtcg
     3721 cgaccaaatg gctggcgggt agcgacggta aatcggccgc agaagcagca accgaacaag
     3781 caggctggag caacggagtt tgatgaaaaa cgtgcgccgc gagaccaatc ggacgcgcct
     3841 tggccgaatc cgacattcgc tgatcgatgc aatttgatca atctaccgtc gctgcggtcg
     3901 aagcctgtcg caggtaagcc ttgacaccac acgggccacg gcagaagcat gtcggcaccc
     3961 gcgacgagag accagcgatg gccaggccat gcacgcgccg ccgcctcatc aaggccggag
     4021 cggctcttat cggaggcgtc gccagcccgg cgatcggctt tccgacgatt ctgcgcgccg
     4081 ccacggtcac gcggttgaaa tgcgtgacgc tcgccaccgg agtgagcgtc atcgtcgacg
     4141 cgtatatgca ggccaagcgc ttcgatctga agaacgacgt cgatctcgtc gccgtcaatt
     4201 cctacacgtc catcgccaac tactacaacg actttgctgc cggcacattc gacgtggcga
     4261 tcggcagctg ggataccttt gcccagatgt atggccgagg catcccgatt cggcttgcgt
     4321 gcacggcggc gtcggcggaa cttgtcagca ttgtcgcggc gaagaacggc cctgcgacga
     4381 tcgaggagct gcgggggaag accctcgccg ccgtccaggc gtccggaagc tataaactct
     4441 cgaaaatggt gatccaggat atctggggca tcgcgctcga gaaggacgtg ccggtcgaga
     4501 acgtgccgaa ccccgcccag gcgctcacca tgatcatggc cgaccgggca gccgcggcgc
     4561 tctcgtggga accgagcgtc agcggcgcca tcgcgcgcgt accggacatg atgccgatct
     4621 tcaatctcgg cgccgcctat cgccagcacc ggcacggggc tctgccgtat ttcacctatg
     4681 cgatccagtc cgccgtcctc aaggggcggc cggatctcgc cgcgcgggtc gccaagtcct
     4741 ttaccgactg cattgccgag atcatggcgg atccgagcga ggccatcgct ctcgccgcgc
     4801 ccgagatgaa gatcgcgcct caggcgctgc gaatcggctt cgattccgga cggttgcaac
     4861 tcaaagccga gccgatgagc aaccccgcaa cccgtcgcgc cgttgcgcgg gccgccgact
     4921 atatgcaccg gaacggcgga cttgctgatt ccgtcgacga cggatttttc gcgctatagc
     4981 ccagcgatgc gagccacggg ccaaaccggc gccgaagagg cgatcgtgac atccgccgcc
     5041 gactctccgg cagtctcgcg gcagagcgat cttctcgccg gcgcgatcgt cgtgctggcg
     5101 atcgtcgtcg tcatgcagat cgcgagcctc gttgtcccgc cctatgtggt gccggcgccg
     5161 ctcgcggtcg cgacggcgct gatcgacgat ctcaggcagg actggctgca cgtcgccatc
     5221 acgctggcgc ggcttggtgc cgccgtcctc gcggcgatga cgatcggcac cgccgtcggg
     5281 gtcgtcatgg ggatgagccc aaggctgcgg ccttatttgc gcgcgctggt cgtcatcgac
     5341 accggcatcc ctgcgctctc ctggatgctg gtcgccgtct tctggttcag gggtcctgag
     5401 cttcgcatct tcttcatcat ggccgtcatc ctcgtcccgt tctacgcctt gaccgtgcat
     5461 gatggaatcc gcgcgctgcc gaaggactgg ctcgagatgt gcgagagctt caggccgtcg
     5521 cgcgcgcaag tccttcgcct cctgatcctg ccgcacatcc tgccctatgt cctgacgacg
     5581 acgcgctcga tgatcggcta tgcgacgcgc atgctgatct tcgccgaggt gatcggctcg
     5641 gcgctgggca tcggcgcgcg catgggcctc gcgcaagcga cctttcacat cgaggacgtg
     5701 ttcgcctgga cggtgcttct cgtcgtgctg aacctcgtgc tccaggctgc gatcaccgcc
     5761 accgagagcc ggctgctgcg ctggcggccg gcgatcgagg tcagatgacg gcgccgccgc
     5821 caagcacgat agagatcagc aacgtgacgc accggttcaa cggtcagctc gcggtcgacg
     5881 atctctcgct cgccgtggct gccggggaga cggtggcgct ggtcggccgg accggcgccg
     5941 gaaagagcac ggcgctccat ttggtcatgg gcacgatcgc tccccatgca ggcacggtcc
     6001 gcgtggccgg agtcgatccc tgtcgcgacg tcgccgcgct ccgcggcaag ctcgccatca
     6061 gcttccagaa cgatcggctg ttgccgtggc gcaccgcggt cgagaatgtc gcgttcggcc
     6121 tggagatcct cggccatgcc aggagcgact gccgccggcg cgcgcgggac tggctcgccc
     6181 gcgtgaagat ggccggtgcc gaggagaaat acgcgcatga gctctcgggc ggcatgcgcc
     6241 agcgcgtctc gctggcgcgg gcactagcgg tcgacccggc gatcctcctc ctcgacgagt
     6301 ccttcagcca gctcgaccat gtcacctcgc aggagctgcg gcgcgacgtg agccggctga
     6361 tccacgcgct cggcaagacc tgcctcttcg tcacgcatcg gatcgacgac gccatcgaga
     6421 tggccgaccg cgtggtggtg gtgagggcgc cggcgcgagt cgcgctcgag gtccggccga
     6481 gcgacgcggt ccgcggcaac cccgagcgcg ccgcgcgact ccatgacgac atcgccgccg
     6541 cgctgggcgg cgatgagcga acgctcgaga gcacatgagt agtcagtgat ccgttgtcag
     6601 tcatcagttc agtgactgct tcactgacaa ctgataactg acaactccgt tccaactaac
     6661 taagcggcgc gcacggtggt caggaactga tcgacctcgt ggttgagcgc ggccgcctcg
     6721 cgggcgagct cgtcggcggc ggcgcggacc tcggacgccg cctttccggc gatgtcgacg
     6781 gcggcgctga cgccggcgat gttgcgcgag acctcgcggg ttccacccgc cgcctcctgg
     6841 acattgcggt tgatctcgcg cgtcgccgcc atttgctcct cgaccgccgc cgcgattgcg
     6901 ctcgagatcg tattcacgtt gaggatcgtc tgcgatatgc ccttgatcgc cgcgacggcc
     6961 gaggtggtct cctgctggat ggcgccgatc tgggcgcgga tgtcgtcggt cgccttcgcc
     7021 gtctgcgtcg ccagcgattt cacctcggcc gcgacgacgg cgaagccctt gccggcatcg
     7081 ccggcgcggg cggcctcgat ggtggcgttg agcgcgagca gattggtctg gctggcgatg
     7141 tcgttgatga gcgccacgac ttcgccaacc ttctgcgcgg cttcggccag gccggccacg
     7201 ctgtcattcg tgtgctgtcc ctccgtcgcc gccttgcgcg cgaccgtggc ggcttgcgtc
     7261 acctggcggc cgatctcgcc gatcgactcc gagagctctt ccgacgccgc ggcaacggtc
     7321 tggacgttgg tcgtcgcctg atcggcaccg gtggcgacgg cgagcgcttg ccgccgcgtg
     7381 gtctcgaccg tctccgccat cgaacgcgcg accccttgca tcgccccggt cgcgcggaac
     7441 accgtaccga caatgccgcg aacccgcgcc tcgaatgtat cggccatcgc cgcgagcgcc
     7501 tttcgcttct cttcctcggc ccggcgcttg agctcgtcct gctccgcccg catctcgcgc
     7561 atggcaatgg cgttgtcctt gaagacccgg accgcgcgcg ccatttcgcc gaactcgtcg
     7621 cggcgctcga cttcttcgac cgccgtggcg aggtggccgg cggccagctt ctccatcttc
     7681 cgcttgaggc tgccgagggg ggccgcgatg ttccggctga tcagataggc gaggaggcac
     7741 accatggcaa taagcgccgt cgcggcgccg ccgagctcca gcagcaccgc gcgatattca
     7801 gcctcgatgt cgtcgatcca cacgccggtg gcgaggatga cgttccaagg ctggtagagc
     7861 cggacgaaag tgagcttcgg catgggcgtg tcctcgccag gcttcttgta atagtaagtg
     7921 agtgctcctt ccttggtcgt ccgggcaatc tcggccatcg agccgtaaag cggcttgccg
     7981 gtcacgtctt tgccttgccg tcgatccgtg tcttcctgct tcaggtcggg cgtgtaaatg
     8041 tagaagtagc cgtccatggt gagcgccaga agatagtcgt ggtgatcgtc gtaccacatc
     8101 gaatgggcat aagtacggaa gcgcgccatc gcctcttcgc gtgtggtctt gccggcgatg
     8161 acctcgtcct cgagcgcgcg cgcaacgcct tcggcgatct cgatgatgcc gcggagcttc
     8221 gcgatgcgat catcgatcat ccgttgatat tgcaaagacg cggccaggac gatggcgagc
     8281 gcgagcgcga ccaccgacac tcccacgagg acgccgagct ttgccttgat cctcaaccga
     8341 tcgagaatga acatggccgt ttctttcgaa cgcgcgggaa agatccgcag gcaggcgtat
     8401 gtcggtgcga atttgcctgc cgctggtgaa ttattggtaa aacacgtcat ccgtgaaatg
     8461 acggcggagc gcgatgcgct gtcggcgtga gcggaacgca acgactcggc gcgcggattt
     8521 gcgccgcgcg ccgtttttca gattcggatt tcgcgggcgc tataacgaaa gtggttgcgg
     8581 tgacccgcag ccactgcaac cgacattcgc cgggcgccgc agtccgatcg cgtcaacctt
     8641 cgctcaggcg gcgcttcgac gggaagcccg ctgtgcgctc tcgggtcaaa ccacggcgcc
     8701 gatcgggtcg ccgaagcgct ctttcatcac cgtctcgaac ggcactccgg ccgccgccac
     8761 ccagctcccc gagcgcaggc ggtaggcggc gtcgcgccag ggggcctcgg gctcccgccc
     8821 ctcgatgagc gccttggcgc cggccaggag caggcggcgg aagcgcacga tcgcgacgtc
     8881 ggtcggggtc agatgctcgc gcgtccggtc ggcgatgccg ccctggctct cctggatcat
     8941 ggcatcctgc tcggcgacac cgcgcacgcc ggtgaagctg cgatgcttct gctcgtcgcg
     9001 gtcgatcatg taatcgttgt cgcggttcct gatcgggacg taatcggggc ccacctcggc
     9061 aagcacgctg tggcccgcgg cgaacttcgc gcgctcggcg tcgctcagcg ggcgctcggg
     9121 gttccaggca taggtataga tccagcagct gtcgtcggtg atggggacga aggtatagcc
     9181 gaagtagttc tcgcccggca gcgtcgaggg cgtggtggcg tgcgcgggaa gcatgaattg
     9241 cgtggtgcgc cagtagaggt cttcgccatc ggccttgcgc gcgccgccgg cgacgaatcc
     9301 gaccgggtgc tcgacgatcg agaagcgcgg catcggatca ttgcggaccc agcgcatccg
     9361 cgtctcgtcg gcggccaccg ccggattccg attcgacgcg acgcctggcg ccggcatgtg
     9421 caggaaagag aaatgacagg tatcgagcgc gccttcgacc gcttgcgccc agttgcattg
     9481 ctgcagcttc ttggtcacga agcgctgctc ggccggcacc acgccgaatt cgagctgagg
     9541 aacttcggga acacgcccgg gcgggccgag ataggcccag acgatgtcgc cgaactcgcg
     9601 cgtcggatag gccttgacgg cgatggtctc gtgatagcgc gagtccggcg gaacattggg
     9661 cagttcgacc gccttgccgg taacgtcgaa cttccagcca tgatagacgc agcgcaagcc
     9721 gcattcctcg ttgcggccat agaatagatt ggcgccgcga tgcgggcatc ggggctccag
     9781 cagcccgata cgtccccggc tgtcgcggaa ggcgacgaga tcctcgccca tgagcctgac
     9841 gcggatcggc ggcgaatccg gcttgggcag ctcgcgcgac aaggcgaagg gctgccagaa
     9901 gcgacgaaag aattgaccca tcggcgtgcc tggtccggtg agggtgagca tgtcgttatc
     9961 ggccgcagcg agcatgggat atctccaatc gagacagtca gggagccgag gggcgtcgtc
    10021 atcttagacg aggacgcggt cggctcggcg gattttcgcc gcggattttc gccacctgtc
    10081 gctgcccggc gccccgggtt ccctgctccg cagacgtcga gatcgaggga gccgtcggga
    10141 gatttggtgg cgggggggaa gggatttgca cctctgcccg ctcttcgccc gcaagacaga
    10201 ggaatacctg gggcgtcgtc ggtatcaggg acttgacggc gaagaaagcc agccggtaag
    10261 attgagaata agtcgcagtt gcgcgccttt agggatatgg aacagtccgt gtatcgcctc
    10321 ttgcctgcca tgacgatcct gacagcgatc attgcggcac cggccatcgc cgaggatagc
    10381 ggcgtcacca ttacgatcaa ggaccatcgc ttcgttcccg acatggtgga gattccagca
    10441 ggaacgaagg tcaagatcgt cgtccgcaac gaggacacga cgacctcgga attcgaaagc
    10501 gtcgctttcc atcgcgagaa ggtggttcaa cccggcagcg agatcacgct gttcgtcggc
    10561 cccctcgacc ccgggagcta cgagttcttc gacgatttcc atcccgaaac gcgcggccat
    10621 ctggtcgcga aataaagagg gccggcatgc tcgcgacgct gatcatcgtc ttccgcgagg
    10681 ttctggaagc tgccctcatc gtcggcatcg tgctggcggc gagccgcggc gtgccgcgcc
    10741 gcgagcaatg gatctcggcc ggcatcgccg gcggggtctt gggtgctctc atcgtcgcgt
    10801 cgctcgccag cgagatcgcc tccgcggtcg aaggcgtcgg ccaggagctg ttcaacgccg
    10861 gaatcctgtt tgccgccgtc ggcatgctgg gatggcacaa catctggatg cagcgccacg
    10921 ggcgcgagct ggcgcagagc gcgacggcga tcggccgcgc ggtgctcgcc gggacgcggc
    10981 cgctctatgc gttggcggtg gtggtcggcg tcgcggtgct gcgggaagga tcggagatcg
    11041 tgctgttcct ctacagcatc gccgttgccg gcggcagcag cgcaaccagc atgattaccg
    11101 gcggcgtgct ggggctcgcc gtgggcgtcg gcgtcggcgc cggactttat ctcggcctgc
    11161 tcgccattcc gcttcgccat ctcttcacgg tgacgagctg gctcatcctg tttctcgcgg
    11221 ccggaatggc ctcgcaaggc gccgcttttc tgcttcaggc cgacctcttg ccggcacttg
    11281 gcaacaatct ctgggacacg tcatttctct tgagcgagcg cagtctgccc ggtcaggtcg
    11341 tgcatgtcct gctgggctac agcgcccagc cggcgggcat ccaggtcgtg ttctacctgg
    11401 cgaccctcgt catcatcggc tcgctgatgc ggctcctcgg cggcatgccc gcgccagcgc
    11461 cgcgacgaga cctggctagg tcataatcgg tcgtcgcctc ggcgccacgt tgcgccacgc
    11521 tgcgctgggg gaacatttga gcgcgaaact ctcgatcggc gcagcggttt cggtctggtc
    11581 gttgtcgctt ctctatgccg gcccggcggc ggccgacttc aaggtcatca cgccggatgc
    11641 caatcgcggc gaatt
//
LOCUS       DASYNS010000059         2373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4071543, whole genome shotgun sequence.
ACCESSION   DASYNS010000059 DASYNS010000000
VERSION     DASYNS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4071543"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VGP50_01865"
     CDS             <1..713
                     /locus_tag="VGP50_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HEV8026143.1"
                     /translation="TLLAPMAAMLGGDRVGRVAISAQTMRACLDSLATRLDEQRDISR
                     YLTGLLVFLGLLGTFWGLIETVGSVGTIINGLKVGGDADSVFDALKEGLAAPLGGMGI
                     SFSSSLFGLAGSLILGFLDLQTSQAQNRFYTNLEDWLATTVEDLGTGLPAAAAAATLP
                     AGVMDAIERMRLSMGDAGSGKATTVAMANLAEAIQGLVQHMRSEQQMIRDWAEAQARL
                     NADIRRFMEILSREAVDR"
     gene            789..1817
                     /locus_tag="VGP50_01870"
     CDS             789..1817
                     /locus_tag="VGP50_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HEV8026144.1"
                     /translation="MALSRLNRDNGMNYWPGFVDALSTLILSIIFLLTVFMVAQFFLS
                     QEVSGKDTALARLNAQISRLSDLLSLERTGKANLEDQLAQLRASLSAAQGERDRLRGA
                     AEGAQNAEAGKAEVGKLSSQLDIEKAATARAVAQIEILNQQISALRRQIGALEDALGT
                     SEKKENDSQLRIADLGQRLNVALAQRVQELSRYRSDFFGKLREILGNRPDIRVVGDRF
                     VFQSEVFFDSGAAVLRPEGRAELDKLAAALLDLDKQIPSEINWVLRVDGHTDTRPITG
                     TSSPFKSNWELSAARAISVVQYLIGKGVSPQRLVAAGFGEFQPIDPGTTEEAFSRNRR
                     IELKLTER"
     gene            1817..2275
                     /locus_tag="VGP50_01875"
     CDS             1817..2275
                     /locus_tag="VGP50_01875"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8026145.1"
                     /translation="MADRTDVVVRALRPEDLDEMLDVWVAAWQAAYREIDFAARREWA
                     RGHIAELERTGSRSLIATCANRIVGAMVVDPATGYLDQIVVATELQGRGIAAALLAQA
                     RRIAPAGLELHVNQDNARAIRFYEKHGFATVGEDVNSRSGAPTFRMRWEP"
BASE COUNT          384 a          797 c          800 g          392 t
ORIGIN      
        1 cgacgctgct ggcgccgatg gcggcgatgc tcggcggcga ccgggtcggg cgggtggcga
       61 tctcggcgca aacgatgcgc gcctgtctcg attcgctcgc cacgcggctc gacgagcagc
      121 gcgacatttc ccgctatctc accggcctcc ttgtgtttct cggcctcctc ggcaccttct
      181 ggggcctgat cgagaccgtc ggctcggtcg gcaccatcat caatgggctc aaggtcggcg
      241 gcgacgccga ttcggtcttc gatgcgctca aggaaggcct cgcggcgccg ctcggcggca
      301 tgggcatttc gttttcgtcc tcgctgttcg gcctcgccgg ctcgctcatt ctcggcttcc
      361 tcgacctgca gacgagccag gcgcagaacc gcttctatac gaatctcgag gactggctgg
      421 cgacgacggt cgaggatctc ggcaccgggc tgccggcggc ggccgccgcc gcgacgcttc
      481 ccgccggggt catggacgcg atcgagcgca tgcgcctctc tatgggcgac gccggatcgg
      541 gcaaggcgac gacggtcgcc atggccaatc tcgccgaggc gatccagggc ctcgttcagc
      601 acatgcgctc ggagcagcag atgatccgcg actgggcgga ggcgcaggcg cggcttaatg
      661 ccgacatcag gcgcttcatg gaaatcctct cgcgcgaagc ggtcgataga tagagcatga
      721 tgcggaaaag cagacgccgg ttttccgacg agatcatgct ccgccaaaga taaagatagg
      781 ccgaggccat ggccctctcc cgattgaacc gcgacaacgg catgaattac tggccgggct
      841 tcgtcgacgc gctgtcgacg ttgatcctgt cgatcatctt tctccttacc gtgttcatgg
      901 tggcgcagtt cttcctgtcg caggaagtct ccggcaagga caccgccctc gcccggctca
      961 acgcgcagat ttcccggctc tccgacctgc tctcgctcga gcgcaccggc aaggccaatc
     1021 tcgaagacca gctggcgcag ctgcgcgcct cgctgtcggc cgcgcagggc gagcgcgacc
     1081 gcctgcgcgg cgcggccgag ggcgcgcaga acgccgaggc cggcaaggcc gaggtcggca
     1141 agctctcctc gcagcttgac atcgagaagg cggcgaccgc gcgcgcggtg gcgcagatcg
     1201 agatcctcaa tcagcagatc agcgcgctgc gccgccagat cggggcgctc gaggacgcgc
     1261 tcggcacctc ggagaagaag gaaaacgact cgcagctgcg catcgccgat ctcgggcagc
     1321 ggctcaacgt ggcgctggcg caacgggtac aggaattgtc gcgctaccgc tcggatttct
     1381 tcggcaagct gcgcgagatc ctcggcaacc ggccggacat ccgcgtcgtc ggcgaccgct
     1441 tcgtgttcca gtcggaggtg ttcttcgaca gcggcgcggc ggtgctgcgg cccgaaggac
     1501 gcgccgagct cgacaagctc gcggccgccc tcctcgacct cgacaagcag atcccctccg
     1561 agatcaactg ggtgctgcgc gtcgacggtc acacagatac gcggccgatc accgggactt
     1621 cttcgccgtt caagtcgaac tgggagctgt ccgccgcccg cgccatctcc gtcgtgcaat
     1681 atttgatcgg caagggcgtc tcgccgcagc gcctggtcgc ggccggcttc ggcgaattcc
     1741 agccgatcga tccgggaacc accgaggaag cgttcagccg caaccggcgc atcgagttga
     1801 agctgacgga gcggtgatgg ccgaccgcac ggatgtcgtc gtgcgcgcgc tgcggccgga
     1861 agacctcgat gagatgctcg acgtttgggt cgccgcctgg caggccgcct accgcgagat
     1921 cgatttcgcg gcgcggcgcg agtgggcgcg cggccatatc gccgagctgg aacggaccgg
     1981 ctcgcgttcg ctgatcgcga cgtgcgcaaa ccggatcgtc ggggcgatgg tggtcgatcc
     2041 cgcgaccggc tatctcgacc agatcgtggt ggcgacggag cttcaggggc gcggaattgc
     2101 cgcggcgctg ctcgcgcaag cgcggcgcat cgcgccggcc ggactcgagc tgcatgtcaa
     2161 tcaggacaat gcccgcgcca ttcgcttcta tgagaagcac ggatttgcga cggtgggcga
     2221 agacgtcaat tcacgctccg gcgcgccgac ttttcgcatg cgctgggagc cgtgaggccg
     2281 tcaggtgccc tgaaactgca gccgggcgag ccgcgcataa agcccgccgg cggcgaccag
     2341 cgcctggtgc gtgccttcct cgacgatgcg gcc
//
LOCUS       DASYNS010000060         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_951134, whole genome shotgun sequence.
ACCESSION   DASYNS010000060 DASYNS010000000
VERSION     DASYNS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951134"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VGP50_01880"
     CDS             complement(<1..302)
                     /locus_tag="VGP50_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEV8026146.1"
                     /translation="MAQDTPFLSAAESSTDTRDAAGRPGRSDATAVRLDALTGVRALA
                     ALWVMMYHFSYIPFGALHLLENVPAVNAGYLGVDLFFILSGFIITHTHRRDAGSLAR"
     gene            497..1624
                     /locus_tag="VGP50_01885"
     CDS             497..1624
                     /locus_tag="VGP50_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEV8026147.1"
                     /translation="MAPESPVKRYRSNLQGELDSAALYRALADAEDNQQLKTVYQRLA
                     AVEEAHAEYWTKRLKGVGATAAGRHPGWRTRVLAWAARRFGPTAVLPLINALERADFA
                     KYDAQPEAVSAGLPADERSHSRVIEAMARPPHGGLSGGALAQLEGRHRSAGGNALRAA
                     VLGANDGLVSNLSLVMGVAGADFSDRTILVTGLAGLIAGACSMAMGEWVSVNSARESY
                     QQQIAIEASELAEFPEEEKQELALIYQAKGLSEQQAKILAERLIGDQRTALDTLAREE
                     LGIDPEELGGSAWVAAGTSFLLFSTGAIVPVIPFFALSGDAAVATSLGLSAAAMFLIG
                     AGTTLFTGRGVVFSGMRQLAIGIAAAAVTYGLGRLLGVSLS"
     gene            complement(1625..1762)
                     /locus_tag="VGP50_01890"
     CDS             complement(1625..1762)
                     /locus_tag="VGP50_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026148.1"
                     /translation="MPDRYKFLAIIAFVVAAILGSQVLFKFYEWNRFQTCATAGGRNC
                     R"
     gene            complement(1829..>2435)
                     /locus_tag="VGP50_01895"
     CDS             complement(1829..>2435)
                     /locus_tag="VGP50_01895"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV8026149.1"
                     /translation="GPEMGDFIRGLHEQHGVVFHLERMASAIDASRVTLDDGTTLAAE
                     LVVVGIGVRPRLELAERAGLALDRGILVNEQLETSVPGIYAAGDAARWPDAYTGERLR
                     IEHWVVAERQGQVAARNMLGRGEHYRAPPFFWSQHYDIAIDYVGHAERWDKIEIEGSI
                     AAHDVRLGYRLGSRLLAVATIFRGRDSLEAEVEMERAVGDG"
BASE COUNT          379 a          856 c          823 g          377 t
ORIGIN      
        1 cgcgcgagcg agccggcgtc gcgccgatgc gtatgcgtga tgatgaagcc gctcaggatg
       61 aagaagaggt cgacgccgag atagccggca ttcaccgccg gcacgttctc gaggagatgt
      121 aatgccccga acgggatgta ggagaaatga tacatcatca cccagagggc ggcgagcgcg
      181 cgcacgccgg taagcgcgtc cagacgcacc gccgtcgcat cgctgcggcc aggcctgccg
      241 gcggcgtcgc gggtgtcggt cgatgactcg gccgcggaga ggaatggggt atcttgcgcc
      301 acggtttctt tgcgtcgggc gatgggcaat tgaagttccg accggaaacg ccccggggtc
      361 gatagcggtt cctaacgatc tctccggctg gagcattgct gcggcaggct tgccgaacgg
      421 caccacccgg catttgcgaa gccgtggccc gacctttagt ctcctccgaa aggggcaagc
      481 cgaagggatt acgctcttgg ctccggaaag cccggtcaaa cgctatcgca gcaatctgca
      541 aggcgagctc gacagcgccg cgctctatcg cgcgctcgcc gacgccgagg acaatcagca
      601 gctcaagacc gtctatcagc gtctcgccgc cgtcgaggag gcgcacgctg aatactggac
      661 gaagcgcctc aaaggggtgg gcgccacggc ggcgggccgc catcccggct ggcgcacgcg
      721 cgtgctggcc tgggcggcgc ggcgcttcgg tcccacggcg gtgctgccgc tcatcaatgc
      781 gctcgagcgt gccgatttcg ccaaatacga cgcgcagccc gaagccgtgt cggcgggact
      841 gccggccgat gagcgctcgc attcgcgcgt catcgaggcg atggcgcgcc cgccccatgg
      901 cggtctctcg ggcggcgcgc tcgcccagct cgagggacgc caccgcagcg ccggcggcaa
      961 tgccttgcgt gccgccgtgc tcggcgccaa tgacggcctc gtctccaatc tgagcctggt
     1021 catgggcgtt gccggcgccg acttctcgga ccgcaccatt ctcgtcaccg ggctcgccgg
     1081 gctgattgcg ggcgcctgct ccatggccat gggcgagtgg gtctcggtca acagcgcgcg
     1141 cgaatcctat cagcagcaga tcgccatcga ggcctcggag ctcgcggaat tcccggaaga
     1201 ggagaagcag gagctggcgc tcatctacca ggccaagggc ttgagcgagc aacaggcgaa
     1261 gatcctcgcc gagcgcctca tcggcgatca gcgcaccgcg ctcgacacgc tggcgcgcga
     1321 ggagctgggc atcgatcccg aggagctcgg cggctccgcc tgggtcgccg cgggcacgtc
     1381 ctttctgctg ttctcgacgg gcgcgatcgt gccggtgatt ccgttcttcg cgctctccgg
     1441 cgacgccgcg gtggccacca gcctcggcct cagcgcggcg gccatgttcc tgatcggcgc
     1501 cggcacgacg ctcttcaccg gccgcggcgt cgtcttctcc gggatgcgcc agctcgccat
     1561 cggcatcgcc gccgccgccg tcacctacgg cctggggcgc ctcctcggcg tgtcgctgag
     1621 ctgactaccg gcaattcctg ccgcccgccg tcgcgcaggt ctggaagcgg ttccactcgt
     1681 agaacttgaa cagcacctgc gagccgagaa tcgcggcgac gacgaacgcg atgatcgcca
     1741 ggaacttgta gcggtcgggc atggcggcgc agcctatggc tgggcccgtg tggtatgcaa
     1801 gcctgctgcg atagcgcgcc cccgaccctc acccgtcgcc caccgcccgc tccatctcga
     1861 cctcggcttc gaggctgtcg cggccgcgga agatggtggc gacggcgaga aggcgcgatc
     1921 ccagccgata gccgagccgc acgtcatggg cggcgatgct gccctcgatc tcgatcttgt
     1981 cccagcgctc ggcgtggccg acatagtcga tcgcgatgtc gtaatgctgg ctccagaaga
     2041 acggcggcgc gcggtagtgc tcgccgcgac ccagcatgtt gcgcgccgcg acctggccct
     2101 ggcgctccgc caccacccaa tgctcgatgc gcagccgctc gcccgtataa gcatcgggcc
     2161 agcgcgcggc atcgcccgcg gcatagatgc cgggcacgct ggtctcgagc tgctcattca
     2221 ccaggatgcc gcgatcgagc gccaggccgg cgcgttcggc aagctccagg cgcggccgca
     2281 cgccgatgcc gaccaccacc agctcggccg cgagcgtcgt gccgtcgtcg agcgtcaccc
     2341 ggctcgcatc gatcgcgctc gccatgcgct cgaggtggaa caccacgccg tgctgctcgt
     2401 gaaggccgcg gatgaagtcg cccatctccg gcccg
//
LOCUS       DASYNS010000061        15116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4072631, whole genome shotgun sequence.
ACCESSION   DASYNS010000061 DASYNS010000000
VERSION     DASYNS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 15116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15116
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4072631"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VGP50_01900"
     CDS             complement(<1..278)
                     /locus_tag="VGP50_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEV8026150.1"
                     /translation="MVEAALRGVVREALARTAVNGLPGAHHFYITFRTRFPGLALPDY
                     LAAQYPEEMTVVIEHQFWDLEVTEERFSVTLSFQNRPERLTIPFDAITA"
     gene            complement(341..674)
                     /gene="ssrA"
                     /locus_tag="VGP50_01905"
     tmRNA           complement(341..674)
                     /gene="ssrA"
                     /locus_tag="VGP50_01905"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            771..1712
                     /gene="thyX"
                     /locus_tag="VGP50_01910"
     CDS             771..1712
                     /gene="thyX"
                     /locus_tag="VGP50_01910"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEV8026151.1"
                     /translation="MPLTPEQKAEIDRLRAEAAPTRRATVPALEEILYAAIPVLDHGF
                     VRVIDYMGDDAAVVQAARVSYGKGTKKVSEDKGLIHYLMRHRHTTPFEMCEIKYHVKL
                     PIFVARQWIRHRTASVNEYSARYSILDNEFYVPAPEQLAAQSRGNRQGRGDVLAGAEA
                     ERVLALLREDAERAYRGYAEMLNEDEAGQAIDPARTGLARELARMNLSLNFYTQWYWK
                     TDLHNLLNFLSLRGDAHAQYEIRAYAEAMMETVERWVPMSAEAFRQHRLGGAQLSASG
                     LAVVKRLIAGEKVTQHESGMSKREWAELMETLGDAEH"
     gene            1944..3929
                     /locus_tag="VGP50_01915"
     CDS             1944..3929
                     /locus_tag="VGP50_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026152.1"
                     /translation="MRCIATAGRRATAAVVLAVLAMGLTSLGTRANDVFAPFVPFGAD
                     TTIPGFPDVIAEFVAIPSANSPGTPAALNEATFLRLRSAVDGEFPHPVDAVVIAQPGF
                     ASTAGLWFNTAAAMVHNASGHTCVSQADAHCRLEVWVVQRRSNLMTDTLGLFVARAAN
                     DPTLALDYYFGPGILNVDPNRPGRFPFSTPPDQLFGRPGSVFRPLAQADVPFEADWGY
                     ETFSGDVLAMISLIKEETGARNIFLAGHSQGGLFTSVFAGTRLASGQLGQDLLAGLIF
                     LDGGPTPPPGGNTATPAAIGALLAEVNALRAGEIPVFGASLGANIIFGQVTLDPGTGA
                     ESAIAGLFSRKTPLAETLFPQSAIGGLAESPGGDAFLEKIRTTNQAWAGLTFDPNPLP
                     AFAPPVLQTPTINLLGAHMGTLGFTPLPGVPLCDPLGAQTTPPCPPNADTIDPNKVYG
                     WLDGGGRGPDPQPSNAEAYINLVGFAPSRTNVVPITVDFPVSGQRTIYGGEETGLFWY
                     QSVRYDADMTLLAEFNQIVIDADNVNINVNKNLISIPIYTAKGPLTPVFAGPPFNLPF
                     DNPYPLVTDFTEINRQGVIQTPTAAALTPLNPAINSFLYNHSNFPMADDSLVGQVLPG
                     QPGANLVADTVIDWIFARARGQAIVPAPFQLGVTDRR"
     gene            complement(4024..5124)
                     /gene="apbC"
                     /locus_tag="VGP50_01920"
     CDS             complement(4024..5124)
                     /gene="apbC"
                     /locus_tag="VGP50_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEV8026153.1"
                     /translation="MSGPSEAQVLAALRQITDPEKGKDIVTLGMVTTAQLRDGHVAFA
                     IEVDAQRGPKLEPLRKAAEKAVEALPGVLSVTAVLTAQKVPASPRPAATPGHAHAAKA
                     MVPGVRAIIAVASGKGGVGKSTVAANLGLGLAANGLRVGLLDADIYGPSLPRMLGISG
                     RPTSRDGKILEPMTNHGVKCMSMGFLVPEDTPMIWRGPMVMSALQQMLRDVAWGELDV
                     MVVDMPPGTGDAQLTMAQQVPLAGAVIVSTPQDIALLDARKGINMFRKVDVPVLGIVE
                     NMSYFLCPHCGERTDIFSHGGARREAERLGVEFLGEVPLDMIIRETSDEGRPIVVAHP
                     ESEYAQTFRRMAARLAETLGGARRPAPRIVVE"
     gene            5281..6429
                     /gene="hflK"
                     /locus_tag="VGP50_01925"
     CDS             5281..6429
                     /gene="hflK"
                     /locus_tag="VGP50_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEV8026154.1"
                     /translation="MSWGNQGGGGGGPWGGGGKSPWGRGPQQPNLEEILRRSQARVRG
                     LLPSGWGRQPVLVLVLALILVVWGLTGFFRVQPDEVGVVLRFGAYNRQVTPGLNYHMP
                     YPIEYAVTPKVTRINTIEIGGTPRDLGGRSPTPGELTESRMLTGDENLVDVYFTVQWQ
                     IGDPQKYLFNIREPEETVKAVAESSMREVIGRTPAVDAIVGDKNTMANETRDLVQKIL
                     DSYDSGILINAVLISHRIDFPSQDVQDAFIDVQSAKTDRDSAVIRAQQYLNSIVPRAK
                     GEAAQVTQQAEAYRQQIVRQAEGDASRFLAVYQAYKQAEDVTARRLYIETMEKVLSGA
                     NKIVLDKSATGSGVLPYLPLPELRGSKATPSANQTPQTPTGSSQTQQR"
     gene            6437..7312
                     /locus_tag="VGP50_01930"
     CDS             6437..7312
                     /locus_tag="VGP50_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEV8026155.1"
                     /translation="MSPRFAVAIVLLIVLVFAGLTMPFIVEQTEQAIVLQFGDPIRNK
                     IEEPGLYFKLPYQSVIIYDKRVVGFDLPPAGVNSADQKPMIVDAYTRYRITDPLKFYQ
                     TVGTEQIARSRLVPIITNSVQNSINHVKLADVVSGKREDTMKQIYESVDAQAKSFGIT
                     ITDVRLRRIDLPPTNSNAIYVRMKSDREREAAQLRAEGDQLAKQITAGADRQKIEILA
                     EAQKSAQIAKGQGDADSIKISADAFGRDKDFFAFYRSLEAYKNALGGQDTTFVLSPDS
                     EFFRYFGTAPKPSQR"
     gene            7322..7516
                     /locus_tag="VGP50_01935"
     CDS             7322..7516
                     /locus_tag="VGP50_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEV8026156.1"
                     /translation="MKDFATALALVLVIEGMLYSLFPEGMKRMVAQMMALPPGTLRVS
                     GLIAACLGVAAIWLIRQGVG"
     gene            7729..9177
                     /locus_tag="VGP50_01940"
     CDS             7729..9177
                     /locus_tag="VGP50_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEV8026157.1"
                     /translation="MALAVVAALSLPAGVAIAKPAPDSFADLAAKLLPAVVNVSTTQT
                     IKSDKVGHVGPEVPQFPPGSPFEEFFKDFFDRNQPHGKPDAVPRRATSLGSGFIIDPS
                     GLVVTNNHVIADADEITVTLQDDTSFKAEVVGRDTKVDIALLRVKSSKPLPSVKFGDS
                     DGTRVGDWVLAIGNPFGLGGSVTAGILSARAREINAGPYDDFLQTDAAINRGNSGGPM
                     FNMAGDVIGINTAIYSPSGGSIGIGFAIPANLAKPVVDQLREFGRARRGWLGVNIQSV
                     TDEIAESLGLDKPKGALIASVRDGGPAQVAGIQAGDVVLTFDGKDVNDMRHLPRIVAE
                     TPIDKTVKVKVWRKRKEHVFDVKVGELNEDDQQAAATPNKQQPPAEVAGSVKALGLSL
                     ANLTPELRERFSLAEDSAGVVVTDVATDSPASEKGMRAGDLIVEVAQEEVKNPGQITA
                     KIDEAKQSGRKSVLLLVDRQGDLRFVALKIEG"
     gene            complement(9214..10152)
                     /gene="serB"
                     /locus_tag="VGP50_01945"
     CDS             complement(9214..10152)
                     /gene="serB"
                     /locus_tag="VGP50_01945"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEV8026158.1"
                     /translation="MPECVLTLIAAPVSAPLAEAAIDIAADALLRLGAHVDGRVWLAP
                     GIACDLAFDDLDVDQADAAARKALAERFGPVAIDLVAQAVAGRRKRLLLADMESTIIA
                     NEMLDELADILRLGPEIAEITRRAMNDEMDFATALRARVALLKGVPESALAAAGERIR
                     ITAGAPALVATMRAHGAYTVLVSGGFRCYTRRIAAELGFDRDMANELLVEDGRLTGFV
                     GEPILGRDAKLSALKRIAAEHSLPLATTLAVGDGANDIAMIEAAGLGIAFHARPAVAA
                     RARRRIEYNDLTALLYAQGYRAEEVVSPQSSVLSPP"
     gene            complement(10159..10545)
                     /locus_tag="VGP50_01950"
     CDS             complement(10159..10545)
                     /locus_tag="VGP50_01950"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEV8026159.1"
                     /translation="MLQQFEGGCHCGRVRFRVTGDLALVSECNCSMCTKKGFLHLIVP
                     PGQFELLSGKDDLVTYEFNTRTAKHLFCRVCGIHAFYIPRSDPDKIDVNARCLDGVDP
                     ATLAPKPFDGRNWEAAIADPPWRARG"
     gene            10628..11581
                     /gene="miaA"
                     /locus_tag="VGP50_01955"
     CDS             10628..11581
                     /gene="miaA"
                     /locus_tag="VGP50_01955"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV8026160.1"
                     /translation="METKIVIVAGPTASGKSALALTLAEALGGTLINADSMQLYRDLT
                     VLSARPGAGEMARVPHRLYGVIDAAEACSAGRWRALALTEIAAARAGGRLPILVGGTG
                     LYLRALLEGMAPVPPVPSASRDAARELHARLGGEAFRAALAALDPEAAQRLPAGDTQR
                     LVRAYEVVSATGRPLADWQRAQETTAELCAAAIVLLPPRGTLYRACDARFLAMAEQGA
                     VAEVEALLARRLDPALPAMKAVGVRELQAFLRGQLSREAAIAQAQQATRRYAKRQYTW
                     FNRQLSDGRSLTKLVVGEQFSESLMPKILSFIRQFLLTVRN"
     gene            11698..13476
                     /locus_tag="VGP50_01960"
     CDS             11698..13476
                     /locus_tag="VGP50_01960"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HEV8026161.1"
                     /translation="MAAEQMIGAEILIKALVDQGVDVIFGYPGGAVLPIYDALFKQNH
                     VRHILVRHEQGAVHAAEGYARSTGKVGVVLVTSGPGATNAVTGLTDALMDSIPLVCLT
                     GQVPTHLIGNDAFQEADTTGITRPCTKHNYLIKDINDMARVLHEAFYVATTGRPGPVV
                     VDLPKDVLLTKGPYRAPETVQHKTYRPQLTPDPERIKEAVALITAAKRPLFYGGGGII
                     NSGERAWKNFGELVRLTGFPCTLTLMGLGAFPASERQFLGMVGMHGTYEANHAMHDCD
                     LMIAVGSRFDDRVTGRLNAFSPNSKKIHIDIDPSSINKNVRVDVPLVGDAGAALEAIL
                     AALKVKRWRPDREALAPWWKEIEGRRARNCLAYAKNGKVIKPQYAIERLYQLTHGKER
                     QTKDIYITTEVGQHQMWAAQFFKFEEPRHWMTSGGLGTMGYGLPAAVGVQIAHPGALV
                     IDIAGEASILMNIQEMSTVCQYRLPVKVFILNNQYMGMVRQWQELMHGGRYSESYTAA
                     LPDFVKLADAFGAIGLRASKPEDVDPIIKEMLAVDRAVIVDMVVDPMENCFPMIPSGA
                     AHNEMLLGPGDRQAGGPVSEEGMVLV"
     gene            13509..14012
                     /gene="ilvN"
                     /locus_tag="VGP50_01965"
     CDS             13509..14012
                     /gene="ilvN"
                     /locus_tag="VGP50_01965"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV8026162.1"
                     /translation="MSSIEKHTIAVLVDNEPGVLARVIGLFSGRGYNIESLTVAEVDR
                     AHSLSRITVVASGTPMIIEQIKAQLSRLVPVHAVRDLTEDGPHVGRELALIKVASTGE
                     RRIESLRLADIFRARVIDSTIESFVFEMTGSTEKLNAFINLMEPLGLVDVSRTGVVAI
                     SRGPARF"
     gene            14033..15052
                     /gene="ilvC"
                     /locus_tag="VGP50_01970"
     CDS             14033..15052
                     /gene="ilvC"
                     /locus_tag="VGP50_01970"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV8026163.1"
                     /translation="MRVYYDRDADVNLIKGKKVLVIGYGSQGHAHAINLHDSGVKDVR
                     VALRPGSATAKKAESARFQVMSPADGAKWADVVMVLTPDELQAKLYNDDLKPNMRPDT
                     ALAFAHGFNIHFKLVEPRPDMDVFMIAPKGPGHTVRSEYARGGGVPCLVAVDQNPSGN
                     ALEIALSYAAAIGGGRAGVIETTFKEECETDLFGEQTVLCGGLSALILAGYETLVEAG
                     YAPEMAYFECLHEVKLIVDLIYEGGLANMRYSVSNTAEYGDYTRGPRIINDGVKAEMK
                     RVLAEIQQGLFAKEWVLENAAGQPSFKAIRRRSAEHDIEKVGEKLRAMMPWIAQNRLV
                     DKSRN"
BASE COUNT         2634 a         5043 c         4951 g         2488 t
ORIGIN      
        1 gcagtgatcg cgtcgaaggg gatggtgagg cgctccggcc ggttctggaa gctcagcgtc
       61 acggagaagc gctcctcggt gacctcgaga tcccagaact gatgctcgat caccaccgtc
      121 atttcctcgg gatattgcgc cgcaagatag tcgggaagag cgagcccggg gaagcgggtg
      181 cggaaggtga tgtagaaatg atgcgcgccc ggcaggccgt tgaccgcggt cctcgccagc
      241 gcttcgcgca ccacgccgcg caacgcggcc tccaccatct tgtcgtatct gaggaggtct
      301 ttcacggtcg ctgcggcacc tgcccggttg gcgagagcga agtggggggt ttctgttgcc
      361 cggtaccccc cggaccgcga gtagctctag gcttttatcc ccgttggggg attaggccgc
      421 gagtgcctca tgtgccaggt tatcgttggc acgtcaggtt tggcccgata tcggcggtac
      481 catgccgggc gacggctcgc acctttatta cgcgcgtcga tcctgattcg cccccaaaag
      541 cccgccgccc tcgcggcgtt ttctgttggt ggaggcgccg ggtactgccc ccgggtccgc
      601 tacgcctatt ccatgcaccg tttatcgcca tagtcggttg cccgacgctt ccaaatatag
      661 gtcttttcca gggaggcgca acccgcaggg ggcgatgtcg agtcgcgctt ggagcgacga
      721 cggcacggct tgtagagtga cggctgccga ttccgacggg tttgccgaaa atgccgttga
      781 cccccgagca gaaggccgag atcgaccgct tgcgcgcgga agccgcgccg acgcgccgcg
      841 ccaccgtgcc ggcgctggaa gagattctct acgcggccat ccccgtgctc gatcacggct
      901 tcgtccgcgt catcgactat atgggcgatg atgccgcggt ggtgcaggcg gcgcgcgtct
      961 cctatggcaa ggggacgaag aaggtgagcg aggacaaggg gctcatccac tatctgatgc
     1021 gccaccgcca caccacgccc ttcgagatgt gcgagatcaa gtatcacgtg aagctgccga
     1081 tcttcgtggc gcggcaatgg atccgccacc gcaccgccag cgtcaacgag tactcggcgc
     1141 gctattcgat cctcgacaac gagttctacg tccccgcgcc cgagcagctc gcagcgcagt
     1201 cgaggggcaa ccgccagggc cggggcgacg tgctcgccgg cgccgaggcg gagcgtgtgc
     1261 tggcgctgtt gcgcgaggac gccgagcgcg cctaccgcgg ctatgccgag atgctgaacg
     1321 aggacgaagc ggggcaggcg atcgacccgg cgcggacggg gctcgcgcgc gagctggcgc
     1381 gcatgaacct gtcgctcaat ttttatacgc aatggtactg gaagaccgac ctccacaacc
     1441 tcctcaactt cttgagcctg cgcggcgatg cccatgcgca atacgagatc cgcgcttatg
     1501 ccgaagccat gatggagacg gtcgagcgct gggtgccgat gagcgccgag gccttccgcc
     1561 agcaccgcct cggtggcgcg cagctctcgg cgagcggcct cgccgtcgtg aagcgcctca
     1621 tcgcgggcga gaaagtcacg cagcatgaaa gcggcatgag caagcgcgaa tgggcggagc
     1681 tcatggagac gctcggcgac gccgaacact aaaggtcgtt gctcatcgag caatcggaca
     1741 gattgtcggt ccaccacaga ttgctgatag cgagacgagg ggagctttgg gcggtcggcc
     1801 gtgaggattt attcaaggtc ctccagcggt ccaatcaacg aatgcacgtc acgcctacat
     1861 cattaagttg cggttgcatc actccacgca ccaacggtat ggttgacgac aaaatcaatc
     1921 aacgagggta ggggaggaag acgatgagat gcatcgcaac cgccgggcgg cgggcgacgg
     1981 ctgccgttgt tctggccgtg ctggccatgg gattgacgag tctcggcacg cgcgcgaatg
     2041 acgtcttcgc gcccttcgtg cctttcggcg cggatacgac gatcccgggc ttccccgacg
     2101 tgatcgccga gttcgtcgcg attcccagcg ccaatagccc ggggacaccc gccgccctca
     2161 acgaagccac attcctgcgc ctgcgcagcg cggtcgacgg cgaattcccg cacccggtcg
     2221 acgccgtcgt gatcgcgcaa cccggcttcg ccagcaccgc cgggctctgg ttcaacaccg
     2281 cggcggccat ggtccacaat gcgagcgggc atacctgcgt cagccaagcg gacgcgcatt
     2341 gccgcctcga agtgtgggtg gtgcagcgcc gcagcaacct catgaccgac acgctcggcc
     2401 tcttcgtcgc gcgcgccgcc aacgacccga cgctggcgct cgactactat ttcgggcccg
     2461 gcatcctgaa cgtcgatcca aatcgcccgg gcaggttccc gttctcgacg ccgcccgacc
     2521 agcttttcgg ccggcccggc tcggtgttcc ggccgctcgc ccaggctgat gtgcccttcg
     2581 aggccgactg gggctacgag accttttccg gcgacgtgct cgccatgatc tccctgatca
     2641 aggaagagac cggcgcccgc aacatcttcc tcgccggcca ctctcagggc ggcttgttca
     2701 cctcggtctt tgccggcacg cggctcgcga gcggacaact cggccaggat ctgctggcgg
     2761 gtctgatctt cctcgatggc gggccgacgc cgcctcccgg cggcaacacg gcgacgccgg
     2821 cggcaatcgg cgccctgctt gccgaggtca atgccttgcg ggccggcgag atcccggtgt
     2881 tcggcgcatc gctcggcgcc aatatcatct tcggtcaggt gaccttggat cccggcaccg
     2941 gcgccgagag tgccatcgcc ggactcttct cgcgcaagac gccgctggcg gagaccctct
     3001 tcccgcaatc ggcgatcggc ggcttggcgg aatcgccggg gggcgacgcc ttcctcgaga
     3061 agatcagaac caccaaccag gcctgggcgg gcttgacctt cgacccgaac ccgctccccg
     3121 ccttcgcccc gccggtgctc cagacgccca ccatcaacct gctcggcgcg catatgggga
     3181 cactcggttt caccccgctc ccgggagttc cgctctgcga tccgctcggg gcgcagacga
     3241 cgccgccttg cccgcccaat gccgatacga tcgatcccaa caaggtctat ggctggctcg
     3301 acggcggcgg cagaggtccg gacccccagc catcgaacgc cgaggcctat atcaatctgg
     3361 tgggcttcgc gccctcgcgc accaacgtgg tgccgatcac ggtcgatttt ccggtgagcg
     3421 ggcagaggac catctacggc ggcgaagaga ccggcctctt ctggtatcag tcggtgcgct
     3481 atgacgccga catgacgttg ctcgcggagt tcaatcagat cgtgatcgac gccgacaacg
     3541 tgaacatcaa cgtcaacaag aacttgatct cgattccgat ctacaccgca aaaggcccgc
     3601 taacgcctgt ctttgccggg ccccccttca accttccctt cgacaacccc tatccgctgg
     3661 tgacggactt caccgagatc aaccggcagg gcgtgatcca gacgcctacc gccgcggcgc
     3721 tgacgccgct caatccggcg atcaattcgt ttctctacaa ccacagcaac ttcccgatgg
     3781 cggatgattc gctggtcggc caagtgctgc cgggccagcc cggcgcgaat ctggtggcgg
     3841 acacggtcat cgactggatc ttcgcccgcg cccgcggcca ggccatcgtc ccggcgccgt
     3901 tccagctcgg cgtgaccgac aggcgataaa tcatctcaga ctgtgcaagc gccgcccttc
     3961 gcgaaagcga agggcggctt tttgctgcgg tggcgtgccc gccggcgacg acgccgcgcg
     4021 ggcttattca acgacgatgc gcggcgccgg gcgccgcgcg ccgccgagcg tctcggcgag
     4081 gcgggccgcc atgcggcgga aggtctgcgc gtattcgctc tcgggatgcg cgacgacgat
     4141 cggccgaccc tcgtcggagg tctcgcggat gatcatgtcg agcggcacct cgccgaggaa
     4201 ctcgacgccg agacgctcgg cctctcggcg cgccccgcca tggctgaaga tgtcggtgcg
     4261 ctcgccgcaa tggggacaga ggaaatagct catgttctcg acgatgccga gcacgggcac
     4321 gtccaccttg cggaacatgt tgatgccctt ccgcgcatcg aggagcgcga tgtcctgagg
     4381 cgtcgagacg atgacggcgc cggcgagcgg cacctgttgc gccatggtga gctgcgcatc
     4441 gccggtgccg ggcggcatgt ccaccaccat gacgtcgagc tcgccccacg cgacgtcgcg
     4501 cagcatctgc tggagcgcgc tcatcaccat gggaccgcgc cagatcatcg gcgtgtcctc
     4561 gggcacgaga aaacccatcg acatgcattt gacgccatgg ttggtcatcg gctcgaggat
     4621 cttgccgtcg cgcgaggtcg ggcggccgga gatgccgagc atgcgcggca gcgatggccc
     4681 gtagatgtcg gcatcgagca gtccgacgcg gagaccattc gcggcaaggc cgagcccgag
     4741 attggcggcg accgtcgatt tgccgacgcc gcccttgccg gaggcgacgg cgatgatcgc
     4801 gcggacgccc ggcaccatcg ctttcgccgc atgcgcatgg ccgggcgttg cggcggggcg
     4861 gggcgacgcg ggcactttct gggccgtcag caccgccgtc accgaaagca cgcccggcag
     4921 cgcctcgacg gctttctccg ccgctttgcg cagcggctcg agcttcgggc cccgttgcgc
     4981 gtcgacctcg atggcgaagg cgacatggcc atcgcgcagc tgcgcggtcg tgaccatgcc
     5041 gagggtcacg atgtccttcc ccttctcggg atcggtgatc tggcgcagcg cggcgagcac
     5101 ctgcgcctcg cttggaccgc tcatggctcc ctcgcgtcct tgacttccgc ggcctgcgcc
     5161 cctatccgga gcgggactcg cgtgcccact gcctcttctt gtgggaatgc tgtcataagg
     5221 gaaaatagga aaaagccggc gatttgcaaa gcgagcatgc gcggcgacag gagcacctag
     5281 atgtcgtggg gcaaccaggg aggaggtggc ggcggtccat ggggcggcgg cggaaagagc
     5341 ccgtgggggc gcgggccgca gcagcccaat ctcgaggaga tcctgcgccg cagccaggcg
     5401 cgggtgcggg gtctcttgcc gagcggttgg ggccgccaac cggtgctcgt gctcgtcctc
     5461 gcgctcatcc tcgtcgtctg gggcctcacc ggcttcttcc gcgtgcagcc cgacgaggtc
     5521 ggcgtcgtcc ttcgcttcgg cgcctataac cgtcaggtaa caccgggact caactatcac
     5581 atgccctatc cgatcgaata tgcggtcacg cccaaggtga cgcgcatcaa cacgatcgag
     5641 atcggcggca cgccgcgcga tctgggcgga cgcagcccga cgccgggcga gctcacggaa
     5701 tcgcgaatgc tgaccggcga cgagaacctc gtcgacgtct atttcaccgt gcagtggcag
     5761 atcggcgacc cgcagaaata cctcttcaac atccgcgagc cggaggagac ggtgaaggcg
     5821 gtcgccgaga gctcgatgcg cgaggtgatc ggccggacgc ccgccgtcga cgccattgtc
     5881 ggcgacaaga acacgatggc gaatgagacg agggatctcg tgcagaagat cctcgacagc
     5941 tatgacagcg gtatcctgat caacgccgtc ctcatcagcc accgtatcga tttcccctcc
     6001 caggatgtcc aggacgcctt catcgacgta cagagcgcca agaccgaccg cgacagcgcg
     6061 gtgatccggg cacagcaata cttgaacagc atcgtgccca gggccaaagg cgaggcggcc
     6121 caggtcacgc agcaggccga agcctatcgc cagcagatcg tgcgccaggc ggagggtgat
     6181 gcgagccgct ttctcgccgt ctatcaagcc tataagcagg ccgaggacgt gacggcgcgg
     6241 cggctttaca tcgagacgat ggagaaggtc ctgtccggcg ccaacaagat cgtgctcgac
     6301 aagtcggcga cggggtccgg cgtgctgccc tatctgccgc tgcccgagct tcgcggatcg
     6361 aaagcgacgc cttcggcgaa ccagacaccg cagaccccta cggggtcgag ccaaacgcag
     6421 caacggtgag gacgccatga gcccgcgttt cgccgttgcc atcgtcctct tgatcgttct
     6481 ggtcttcgcc ggcttgacca tgcctttcat cgtcgagcag acggagcagg cgatcgtgct
     6541 gcagttcggc gatcccatcc gcaacaagat cgaagagccc ggcctctact tcaagctgcc
     6601 ctatcagagc gtcatcattt acgacaagcg cgtggtgggg ttcgacctgc cgcccgccgg
     6661 tgtcaactcg gcggaccaga agccgatgat cgtcgacgcc tatacgcgct accgcatcac
     6721 cgatccgctg aaattctacc agaccgtcgg caccgagcag atcgcgcgca gccggctcgt
     6781 gccgatcatc accaacagcg tgcagaactc gatcaatcac gtgaagctcg ccgacgtcgt
     6841 ctcgggcaag cgcgaagaca cgatgaagca gatctacgag agcgtcgacg cacaggcgaa
     6901 gtccttcggc atcaccatca ccgacgtgcg gctgcgccgc atcgacctgc cgccgaccaa
     6961 cagcaacgcc atctatgtgc gcatgaaatc cgatcgcgag cgcgaggcag cgcagctccg
     7021 ggccgagggc gaccagctgg cgaagcagat caccgccggc gccgaccgcc agaagatcga
     7081 gatcctggcc gaagcccaga agtcggcgca gatcgccaag ggtcagggcg atgccgacag
     7141 catcaagatc tcggccgacg ccttcgggag ggacaaggac ttcttcgcct tctaccgctc
     7201 gctcgaggcc tacaagaacg cgctcggcgg ccaggacacg accttcgtgc tgtcgcccga
     7261 cagcgagttt ttccgctact tcggcacggc gccgaagccg tcgcagcgct gagaggcggt
     7321 catgaaggac tttgccacgg cgcttgcgct cgtcctggtc atcgagggca tgctctattc
     7381 gctcttcccc gaagggatga agcgcatggt ggcgcaaatg atggcgctgc cgccaggcac
     7441 gctcagagtg agtggcctca tcgccgcctg ccttggcgtc gccgccatct ggctcatacg
     7501 ccagggtgtc gggtgaggtg aaggcgcctt aatttgaccg cattcaaggc gctagctctg
     7561 cggccgtctg gcccgccttt gcatgctttt ccggaagcgg ctggtttacg gcggcaatct
     7621 cccgtaaaat cggacatcct cgctccgcct gccctaggga gtgcattcgt gagtcgatcc
     7681 attgctcgtc ggatagatcg ttcggcgccg cgccgctcgc gcgtcgtcat ggcactggcc
     7741 gtggttgcag cgttgtcgct gcctgccggt gttgccatcg ccaagccggc gcccgacagc
     7801 tttgccgatc ttgccgcgaa gctcctgcct gcagtggtca acgtctcgac cacgcagacg
     7861 atcaagagcg acaaggtggg ccatgtcggc ccggaagtcc cgcaattccc gccgggctcg
     7921 cctttcgagg agttcttcaa ggacttcttc gaccgcaacc agccgcatgg caagcccgat
     7981 gcggtgccgc gccgcgctac ctcgctgggc tcgggcttca tcattgaccc gtcgggactg
     8041 gtggtgacga acaaccacgt catcgccgat gccgacgaga tcaccgtcac ccttcaggac
     8101 gacaccagct tcaaggcgga ggtggtgggg cgcgacacca aggtcgacat cgccttgctg
     8161 cgcgtcaaat cgagcaagcc gctgccctcg gtcaagttcg gcgacagtga cggtacgcgc
     8221 gtcggcgact gggtgctggc gatcggcaac cccttcggcc tcggcggctc ggtcacggcc
     8281 ggcatcctct cggccagagc ccgcgagatc aacgccgggc cctatgacga cttcctgcag
     8341 accgatgccg ccatcaaccg cggcaactcg ggcgggccga tgttcaacat ggcgggggac
     8401 gtgatcggca tcaacaccgc gatctactcc ccctcgggcg gctcgatcgg catcggcttc
     8461 gccatcccgg ccaatctggc gaagcccgtg gtcgatcagc tcagggagtt cggacgggcg
     8521 cggcgcggct ggctcggcgt caacatccag agcgtcaccg acgagatcgc cgagagcctc
     8581 ggcctcgaca agcccaaggg cgcgctcatc gccagcgtgc gcgacggcgg gccggcccag
     8641 gtcgccggca tccaggcggg cgacgttgtg ctcaccttcg acggcaagga cgtcaacgat
     8701 atgcggcatc tgccgcggat cgtcgccgag actcccatcg acaagaccgt caaggtcaag
     8761 gtctggcgca agcgcaagga gcatgtcttc gacgtcaagg tcggcgagct caacgaggac
     8821 gaccagcagg ctgcggccac gcccaacaag cagcagcccc cggcggaggt cgccggctcg
     8881 gtcaaggcac tgggtctctc gcttgccaat ctcacgccgg agctgcgcga acgcttctcg
     8941 ctggcggagg attccgccgg cgtcgtggtg actgacgttg ctaccgacag cccggcctcg
     9001 gagaagggca tgcgcgccgg cgatctcatc gtcgaggtgg ctcaggaaga agtgaagaac
     9061 cccggccaga tcaccgccaa gatcgacgag gcgaagcagt ccggccgcaa atcggtgctg
     9121 ctgctcgtcg accgccaggg cgacctgcgc ttcgtcgccc tcaagatcga ggggtaggtc
     9181 ggagtgatca gtcgtcagta ttcagttgtc agttcacggc ggactgagaa ctgacgactg
     9241 aggactgacg acttcctcgg cccgataccc ctgcgcataa agcagcgccg tcaggtcgtt
     9301 gtactcgatg cgccggcgcg cccgtgcggc gactgccgga cgggcgtgaa aggcgatacc
     9361 gaggccggcc gcctcgatca tggcgatgtc gttggccccg tcgccgaccg ccagcgtcgt
     9421 ggcgagcggc agggaatgct cggcggcgat ccgcttcaag gccgaaagct tggcatcgcg
     9481 gccaaggatc ggctcgccca cgaagccggt gagccgcccg tcctcgacga ggagctcgtt
     9541 ggccatgtcg cggtcgaagc ccagctcggc ggcgatgcgg cgcgtgtagc agcgaaagcc
     9601 gcccgagacg agcaccgtat aggccccatg cgcgcgcatg gtcgcgacca gcgccggcgc
     9661 gccggcggtg atgcggatgc gctcgccagc cgccgcaagc gcgctctcgg ggacgccttt
     9721 caaaagcgcc acgcgcgcgc gaagcgccgt ggcgaaatcc atctcgtcat tcatggcgcg
     9781 gcgcgtgatt tcggcgatct ccggccccaa gcgcaggatg tcggcgagct cgtcgagcat
     9841 ctcgttggcg atgatggtcg attccatgtc ggccaggagc agccgcttgc gccggcccgc
     9901 cacggcctga gccacgagat cgatggcgac cgggccgaag cgctcggcaa gcgccttccg
     9961 cgcagcggcg tcggcctggt cgacatcgag atcgtcgaag gcgagatcgc aggcgatgcc
    10021 gggagcaagc cagacccggc catccacatg cgcgcccaga cgcaacaagg cgtcggccgc
    10081 gatgtcgatc gccgcctcgg ccaagggggc cgatacgggg gcggcgatga gcgtaagcac
    10141 gcattccggc atcgcaggct atccgcgcgc gcgccaaggc ggatctgcga tggctgcttc
    10201 ccagttccta ccgtcgaaag gcttgggtgc cagcgtcgcg ggatcgacgc cgtcgaggca
    10261 gcgcgcattc acgtcgatct tgtcggggtc cgagcgcggg atatagaaag cgtgaatgcc
    10321 acagacgcgg cagaagagat gcttcgccgt ccgcgtgttg aattcgtaag tgacgaggtc
    10381 gtccttgccg ctcaggagct cgaactgccc ggggggcacg atcagatgca ggaagccctt
    10441 cttcgtgcac atcgagcaat tgcactcact gaccagcgca agatccccgg tcacgcggaa
    10501 ccgcacccgg ccgcaatggc acccgccctc gaattgctgc agcatggcgc tctatagctg
    10561 gatcaatgcg cgcgcgtcac cccccaccct tgagggggag ggcaggggtc gggggtctcg
    10621 cagcacggtg gaaacgaaga tcgtcatcgt cgccggaccg acggcgagcg gcaaatcggc
    10681 gctggcgctg accttggccg aggcgctggg cggcacgctc atcaatgccg acagcatgca
    10741 gctttatcgc gatctcaccg tgctctcggc gcggccgggt gccggcgaaa tggcgcgcgt
    10801 gccgcatcgc ctctatggcg tcatcgatgc cgcggaggcc tgctcggccg ggcgctggcg
    10861 cgcgctggcg ctgaccgaga tcgccgccgc tcgagcggga ggccggctgc cgatcctggt
    10921 gggcggcacc gggctctatc tacgcgcgct cctcgaaggc atggcgccgg tgccgccagt
    10981 gccgtccgcg tcgcgcgacg cggcgcgcga gctccacgcg cggctcggcg gcgaggcttt
    11041 ccgggcagcg ctggcggccc tcgatcccga ggcggcgcag cgtctgccgg ccggcgacac
    11101 ccagcggctc gtccgcgctt acgaggttgt gtccgcaacc ggccggccgc tcgccgactg
    11161 gcagcgcgcc caggaaacga cggccgagct ctgcgccgcg gcgatcgtcc ttctcccgcc
    11221 tcgcggcacg ctctatcgag cctgcgacgc ccgctttctc gccatggccg agcagggggc
    11281 agtcgccgag gtcgaggccc tcctcgcccg gcggctcgat ccggcgcttc cggcgatgaa
    11341 ggcggtcggc gtgcgggagc tgcaggcgtt cctccggggg cagctgtcgc gcgaggccgc
    11401 gatcgcccag gcgcagcaag cgacgcgccg ctacgccaag cgccagtaca cctggttcaa
    11461 ccgccagttg agcgacggcc gcagcttgac gaaactcgtc gtcggcgagc aattttcgga
    11521 aagtctgatg cccaaaattt tgtcgtttat tcgtcagttc ctattgaccg ttaggaatta
    11581 gctctctagg gttgccgctt tcgcgccgga cgtaagggtc caatgggggc ggcggccaga
    11641 gcggccagcc gcagcggata tccttagtcc cgcgcgattt ttgggctgga gagcgtgatg
    11701 gcggcggagc agatgatcgg ggcggagatc ctcatcaagg cgctggtcga ccagggcgtc
    11761 gatgtgattt tcggctatcc cggcggcgcc gtattgccga tctacgacgc gcttttcaag
    11821 cagaaccatg tgcgccacat tcttgtccgc cacgagcagg gggcggttca cgccgccgag
    11881 ggctatgcgc gctcgaccgg caaggtcggc gtggtcctgg tgacgagcgg ccccggcgcc
    11941 acgaacgcgg tgacgggact gaccgacgcg ctcatggatt cgattccgct ggtgtgcctg
    12001 acggggcagg tgccgaccca tctcatcggc aacgacgctt tccaggaggc ggacacgacc
    12061 gggatcaccc gtccctgcac caagcacaac tacctgatca aggacatcaa cgacatggcc
    12121 cgtgtccttc acgaggcctt ctatgtcgca accacggggc ggcccggccc cgtggtggtc
    12181 gacctgccca aggacgtgct gctgacaaag gggccttatc gcgcgcccga gacggtgcag
    12241 cacaagacct accgcccgca gctcacgccc gatcccgagc ggattaagga ggcggtggcg
    12301 ctcatcacgg cggcgaagcg gccgctcttc tatggcggcg gcggcatcat caattcgggc
    12361 gagcgcgcct ggaagaattt cggcgagctg gtgcgcctca ccggctttcc ctgcacgctg
    12421 acgctgatgg ggctgggcgc gttccccgcg tccgagcgcc aattcctcgg catggtcggc
    12481 atgcatggca cctacgaggc aaatcacgcc atgcatgact gcgatctcat gatcgcggtg
    12541 ggctcgcggt tcgacgaccg cgtcaccggt cggctcaacg ccttctcgcc caactcgaaa
    12601 aagatccaca tcgacatcga cccgtcctcg atcaacaaga atgtgcgagt cgacgtgccg
    12661 ctggtgggcg atgcgggcgc tgcgctcgag gcgatcctcg ccgctctcaa ggtcaagcgc
    12721 tggcggcccg atcgcgaagc gctggcgccg tggtggaagg agatcgaggg ccggcgggcg
    12781 cgcaactgcc tcgcctatgc gaagaacggc aaggtcatca agccgcaata cgcgatcgag
    12841 cgcctctatc agctgacgca tggcaaggag cgccagacca aggacatcta catcaccacc
    12901 gaggtcgggc agcaccagat gtgggcggcg cagttcttca aattcgagga gccgcgccat
    12961 tggatgacgt cgggcgggct cggcaccatg ggctatgggc tgcccgccgc cgtgggcgtg
    13021 cagatcgcgc atcccggcgc cctcgtcatc gacatcgccg gcgaagcgtc gatcctcatg
    13081 aacatccagg agatgtcgac ggtgtgccag taccggctgc cggtcaaggt cttcatcctc
    13141 aacaaccagt acatggggat ggtgcggcag tggcaggagc tgatgcatgg cggccgctac
    13201 tccgagagct acaccgcggc actcccggac ttcgtcaagc tcgccgacgc cttcggcgcg
    13261 atcggcctgc gcgcgagcaa gcccgaggac gtcgatccga tcatcaagga gatgctcgcc
    13321 gtcgaccgcg cggtgatcgt cgacatggtg gtcgatccga tggagaactg cttcccgatg
    13381 atcccctcgg gcgcggcgca taatgagatg ctgctgggac cgggcgaccg ccaggcgggc
    13441 ggtccggtat cggaagaggg aatggtgttg gtatagcgcc tcaacctttt ccgagtcgga
    13501 gtttcttcgt gagcagcatc gagaagcaca ccattgccgt cctggtggac aacgagcccg
    13561 gcgtcctcgc ccgggtgatc ggcctcttct ccgggcgcgg ctacaacatc gagagcctga
    13621 ccgtggccga ggtcgatcgc gcgcatagcc tctcgcgcat cactgtcgtc gcctcgggca
    13681 cgcccatgat catcgagcag atcaaggcgc agctctcgcg cctggtgccg gtgcatgccg
    13741 tgcgcgacct gaccgaggac gggccccatg tcgggcggga gctcgcgctc atcaaggtgg
    13801 cgagcaccgg cgagcggcgc atcgagtcgc tccggctcgc cgacattttc cgcgcccgcg
    13861 tcatcgacag caccatcgag agcttcgtct tcgagatgac gggctcgacc gagaagctca
    13921 acgccttcat caacctgatg gagccgctgg gcctcgtcga cgtgtcgcgc accggcgtcg
    13981 tcgcgatctc gcgcggcccg gcgcgctttt agctcaccaa gagggacatc ccatgcgcgt
    14041 ttattacgat cgtgacgccg acgtcaatct gatcaagggc aagaaggtgc tggtgatcgg
    14101 ctatggcagc cagggccatg cccatgcgat caacctccac gacagcggcg tcaaggacgt
    14161 gcgggtggcg ctgcgccccg gctcggcgac cgccaagaag gcggagagcg cgcgcttcca
    14221 ggtgatgagc ccggccgatg gcgcgaaatg ggccgatgtg gtgatggtcc tcacgcccga
    14281 cgagctccag gcgaagctct acaacgacga cctcaagccg aacatgcgcc ccgacacggc
    14341 gctcgccttc gcgcatggct tcaacatcca tttcaagctg gtcgagccca ggcccgacat
    14401 ggacgtcttc atgatcgcgc cgaaagggcc cgggcatacc gtgcgctcgg aatatgcgcg
    14461 cggcggcggc gtgccctgcc tcgtcgcggt cgaccagaat ccgtcgggca acgccctcga
    14521 gatcgcgctg tcctatgccg cggccatcgg cggcggccgc gccggcgtga tcgagaccac
    14581 cttcaaggag gaatgcgaga ccgacctctt cggcgagcag accgtgctct gcggcggcct
    14641 gtccgcgctc atcctcgccg gctacgagac tctggtcgag gccggctatg cgccggaaat
    14701 ggcctatttc gagtgcttgc acgaagtgaa gctgatcgtc gacctcatct atgagggcgg
    14761 gcttgccaat atgcgctact ccgtgtcgaa caccgccgag tatggcgact acacgcgggg
    14821 gccgcgcatc atcaatgacg gggtcaaggc ggagatgaag cgcgtcctcg ccgagatcca
    14881 gcagggcctt ttcgccaagg aatgggtcct cgagaacgcc gccggccagc ccagcttcaa
    14941 ggccatacgc cgccgctccg ccgagcatga catcgagaag gtcggcgaga agctccgcgc
    15001 catgatgccc tggatcgccc agaaccggct ggtggacaag agccggaact gatccgcgat
    15061 ggggtcggcg gggtgcgccc ttcgactctt ctcagggtga ggtagatttt ttatgc
//
LOCUS       DASYNS010000062        10808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4853205, whole genome shotgun sequence.
ACCESSION   DASYNS010000062 DASYNS010000000
VERSION     DASYNS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10808
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4853205"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..981
                     /locus_tag="VGP50_01975"
     CDS             199..981
                     /locus_tag="VGP50_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8026164.1"
                     /translation="MTRERAPVLATSDLTIRFGGQIAVNGVSCAFTEGSLTAIVGPNG
                     AGKTTYFNLVSGQLRPSAGSVRFLGRDVTPLGAPERTRLGLGRSFQLTNLYPSLSVLE
                     NVRLAVQSVARIRFDMLGIAVRNRRLVDEARAYLGRVNLEKQALLPAAALSHGDQRKL
                     EVAILLALKPRVFLFDEPTAGMALDEIPLILDLIEAIKAEGDKTILLVEHRMDVVERL
                     ADRIIVLQDGILIADGLPADVVALPAVQQAYLGLHAAEALHA"
     gene            974..1708
                     /locus_tag="VGP50_01980"
     CDS             974..1708
                     /locus_tag="VGP50_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8026165.1"
                     /translation="MPESSDALLRLEGVHTDIGRYQILGGVDLAVGRGRVTMLLGRNG
                     AGKTTTLRSIMGLWKPRAGRISFDGRPISGMTTPDIARLGIAYVPENLGIFGKLTVRE
                     NLLLATGRKGFAEPRLERVLKTFPALKVKWNASAGLLSGGQKQMLAIARAIIEPHPML
                     LVDEPTKGLAPSVVDHLIEALKTLKSEAATILLVEQNFRVARAVGDEVAVMDQGRIIH
                     SGAMAALAADERLQQRLLGLSLGAVA"
     gene            1705..2697
                     /locus_tag="VGP50_01985"
     CDS             1705..2697
                     /locus_tag="VGP50_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8026166.1"
                     /translation="MTASWPGTIEKWAPYAVTPLLMIAALPAMGFGPWLTLTVAGLCM
                     GMMLFLMASGLTLIFGLMNVLNFAHAAFVTTGAYIAASVLWRLTDWTMEPSLALNLAA
                     LAVALVVAAVASGALGYVFERLVIRQVYGAPLRQILITVGALAVVEQLIIVVWGPGAI
                     PLPRPAMLRGSVFIGGSAIETYRLVALGVGLLVAAAMHLVLTHTRIGLLVRAGVEDRE
                     MVEALGYRVHRLFIGVFIAGTALAGMGGMMWGQYQTLVNSQIGNGMTILVFIILIIGG
                     LGSVGGCFIGAVLVGLMSNYVGFLAPKLALGSNLLLLVPILLWRPRGLFPIAEH"
     gene            2705..3844
                     /locus_tag="VGP50_01990"
     CDS             2705..3844
                     /locus_tag="VGP50_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8026167.1"
                     /translation="MSQQPRAALDEPAKAAPVAAGGASRVRSVSRWLVSDDAPGRWWL
                     NAVFLLILLALLVAPFLFPGTKALGVASRICIFVILVASYDLMLGYTGIVSFAHTTFF
                     GIGAYCVAILLAKGWVGWGAVILGALAGVLVSFAVAAVIGMLSLRVRAIFFAMITLAV
                     ASFAQIVASQWRDLTGGEDGLTFSLPGLLMPSFRVFADPVLGVTVNGAVVTYYLTLAV
                     SVVLLLLMLRIVNSPFGRVLQAIRENEFRVEAMGYRVVAYRSWACVLSAGFAALAGAL
                     MALELRYVGPDATLSFSLMVDILLMIVIGGMGTLYGAVIGAILVVLAQYYLQNVLGMV
                     SGAFAGMPVLSALFHPDRWLLWLGLLFVLIVTFFPQGIVGRLRRL"
     gene            complement(3890..4045)
                     /locus_tag="VGP50_01995"
     CDS             complement(3890..4045)
                     /locus_tag="VGP50_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026168.1"
                     /translation="MRAWPVLAFCAFLLAACGCSCPPPTVVVPQGSTVVCPNGSTATA
                     TSSGYRC"
     gene            complement(4184..4951)
                     /locus_tag="VGP50_02000"
     CDS             complement(4184..4951)
                     /locus_tag="VGP50_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026169.1"
                     /translation="MKTFASPKTEQTATLPISAEAVEAFPAMIKRIAATHCPSRMPFF
                     RHLAALPPGAANDPKLLGQIHLVYQSAMHATRAAVYYLPHLDSPALRKRKLQIFVDDD
                     GLPGGDTHHYQLTRAFRNIGAKLLLEDEAFGESDALCRHLDSDTADFVRLAQHLYARS
                     LGPWCAVELMSDDWMRALADALAVNFPNIVKEPYFADCFSHGVEVRHAEESLSVTMLV
                     LRDRHELMPESVRDAQLMAEALDRVWLRLDRIVLGAG"
     gene            5116..6270
                     /locus_tag="VGP50_02005"
     CDS             5116..6270
                     /locus_tag="VGP50_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8026170.1"
                     /translation="MSSSALRLGCAWLTLFVIGTDLFVVSPLLPSVAADLAVAPARSG
                     WCVTVFSLTYMVMAPALGRAADAFGRRRLLLVALAGFAAANLCSGLTRDFAGLLAARI
                     AAGAAAAGVTPSIYALVGDCAPEERRATWFAVAVSGLLLSLALGAPLGTLLAHAFGWQ
                     SVFVAVAVASSLLLPLNRMAWPSSSAAAPATSGDGESGFLVMALRLAPTAVWSTALYA
                     VYTYLGIGLAAWGYAADRIAGVVLCYGAGAVVGALCGGRLADRFGARRICFVSLGGLG
                     AALLALGPALRAGVPMELLLAVLSALAQLFFPAQQAGLARDFAAARATAMAWNNSALF
                     LGIGAGSLIGGAVMARGDLASVATVSAVIALAGAALVRAVAPRPAPARPV"
     gene            6490..8301
                     /locus_tag="VGP50_02010"
     CDS             6490..8301
                     /locus_tag="VGP50_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026171.1"
                     /translation="MTSRLFMLVLIAVLPAIGIQAYNEFDLRRSREADIRQQVIQITR
                     QFGEEMGELREGARQLLLAMSQLPVAQSGHGAECSKLFASLRRKFPNYSLLGAADVKG
                     NVFCASRMVSHWNVADQPFFTRAMARDGVAVGNYFVDSETKEKEIHFAHRFYDASGEV
                     AGVVFAGLDLAWLSDHLKERGLSPTASILIADREGNIIARLPNPEKFVGNNMRGGHAE
                     IMDGDKAGWEEAVGVDGETRIFGYVPAQLPPYDLFLSAGQSKSEALAPIDNATRRGIL
                     LILLGLTLACFAAWRGGKVFIQRPIGHLLEVTKAWRDGDEKARVEIKDTESELGRLGA
                     AFNDMATALAARHEDLRQLNATLEQRVEQRTKELADAHRLAVLGKLIATVSHELRNPL
                     AVIRNTTFALAEAAKKQGLDVERSIERINRNIGRCVEIISELLNYTRSPQLEFVAQDF
                     DRWLVGILADYDIEDGISLRHELHAGVMVRFDSGQLQRVFVNLIDNARDAVHEAKGTA
                     RDPSYAPRVSVKSRVDGRRIAITVEDNGSGIPAEILPKIFEPLFSTKGSGVGLGLPTV
                     KQIIDRHRGQIEVTSRVGEGTSVAIWLPLAVGQELAA"
     gene            8298..9032
                     /locus_tag="VGP50_02015"
     CDS             8298..9032
                     /locus_tag="VGP50_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8026172.1"
                     /translation="MSGEAFSRVLVIDDDRDLAESVAELLEARGHKVSLAFTGRDGVD
                     LVDQNAFDLVLLDIEMPEKSGVDTFLEIRKKGHDVPIALMTGASVEQHFYDALESGTL
                     CLLRPRSTAADVIAATRGKQPRCVVLVSNNVTALIARIQPFLSQQHCTTVIARSSDEG
                     LKWVSAGLADCLVFDQPLSSAAGLYIYLSLRRSGPMSPTILVTLPNDAPNDATPAIQA
                     MTADILKKPFDPEEVLSWLERRAERT"
     gene            9029..10240
                     /locus_tag="VGP50_02020"
     CDS             9029..10240
                     /locus_tag="VGP50_02020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV8026173.1"
                     /translation="MSDQRFPGGAPGASVPDRPRGVRPLVAAMPFTLIGQVAMAGMGD
                     PDVVPLWYGESDVPTPALIAEAAVASLRAGETFYTFKRGIPELRGAIAAYLAGLYGRA
                     IEVERVIVTSSAMSGIMMLFQALVEPGDNVVVVTPLWPNVVDTVTVLGGEIRAVPLVG
                     SPDGSWRLDLARVMAACDPKTRVIFVNSPGNPTGWVMRSGEAADLLAFTRRRGIWLVA
                     DEVYARFVYDGSRAAPSLLEIAEPEDRVVAVNSFSKAWAMTGWRLGWMVAPREIQDVA
                     DKLVEFNTSCAPPFLQRAGIVALEQGEPVVASLLERCSRGRDIIIRGLNRFPRIHVAA
                     PPGAFYAFCRIDGLTDSLAFAKENLARVKVGVAPGAAFGPAGEGHLRFCFACAPARLE
                     LALERLAPMLG"
     gene            10525..>10808
                     /locus_tag="VGP50_02025"
     CDS             10525..>10808
                     /locus_tag="VGP50_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026174.1"
                     /translation="MVAPAERTVMFLDMRDSTRLYEAIGDVAAEHTLRRAVNLCARQI
                     QAARGAVVRSTGDGLLAVFDDAAAALAAAQQIQIELLKHKIEGIAIGVHGG"
BASE COUNT         1630 a         3584 c         3687 g         1907 t
ORIGIN      
        1 tcgactcgcc caagggacgc gtcatgttcc gcaaggagga ccaccagctc ctgcagccga
       61 tgtatgcctt caagatcaag gtcgacccca aagtgccctg ggccattccc gagctcgtcc
      121 gcgagatctc catcagcgag atgaagatcc cgatcacgaa ccatcgcccg tgagacaacg
      181 ggccgcgggc gggccgccat gacgagggag cgcgcacccg tcctcgcgac cagcgacctc
      241 accatccgct tcggcggaca gatcgcggtc aatggcgtct cctgcgcctt cacggagggc
      301 agcctgacgg cgatcgtcgg ccccaatggc gcgggcaaga ccacctattt caatctcgtc
      361 tcgggccagc tgcggccatc ggccgggagc gtgcgcttcc tcggccggga cgtcacccct
      421 ctcggcgcgc ccgagcgcac gcggctcggg ctcggccgct cgtttcagct caccaatctc
      481 tatccgagcc tcagcgtgct cgagaatgtg cgcctcgccg tgcaatccgt ggcgagaatc
      541 cgtttcgaca tgctcggcat cgccgtgcgc aacaggcgcc tcgtcgacga ggcgcgcgcc
      601 tatcttggcc gcgtcaatct cgagaagcag gcgctgctgc cggcggcggc gctgtcccac
      661 ggcgatcagc gcaagctcga agtggcgatc ctgctggcgc tcaagccgcg cgtcttcctc
      721 ttcgacgagc caaccgcagg catggcgctc gacgagatcc cgctcattct cgatctcatc
      781 gaagcgatca aggccgaggg cgacaagacc attctcctgg tcgagcaccg catggacgtg
      841 gtcgagcggc tcgccgaccg catcatcgtg ctccaggacg gcatcttgat cgccgacggg
      901 ttgccggcgg acgtcgtggc gctgccggcg gtgcagcagg cctatctggg ccttcatgcg
      961 gccgaggctc tccatgcctg agtcgagcga cgcgctgctc cgcctcgagg gcgtgcacac
     1021 ggatatcggc cgctaccaga tccttggggg cgtcgacctc gcagtggggc gcggccgcgt
     1081 caccatgctg ctcgggcgga acggcgccgg caagaccacg actttgcgca gcatcatggg
     1141 gttgtggaag ccgcgcgccg gccgcatcag cttcgacggc cggccgatca gcggcatgac
     1201 gacgcccgac atcgcccggc tgggcatcgc ctatgtgccg gagaatctgg ggatcttcgg
     1261 gaagctgacg gtacgcgaga atctgctgct cgccaccggg cgcaagggct tcgcggagcc
     1321 gcgcctcgag cgcgtgctca agaccttccc ggcgctcaag gtcaaatgga acgccagcgc
     1381 gggcctcctg tcgggaggac agaagcagat gctggcgatc gcgcgcgcca ttatcgagcc
     1441 gcatccgatg ctgctcgtgg acgagccgac caaagggctc gcgccgagcg tcgtcgatca
     1501 tctcatcgag gcgctgaaga cactcaagag cgaggcggcg acgatcctgc tggtcgagca
     1561 gaatttccgc gtggcgcgcg cggtgggcga cgaggtcgcg gtgatggacc agggacgcat
     1621 catccatagc ggcgccatgg cggcgctcgc cgcggacgag cggctgcagc agcggctcct
     1681 cggcctcagc ctcggggccg tggcgtgacc gcgagctggc ccggcacgat cgagaagtgg
     1741 gcgccttacg ccgtgacgcc gctcttgatg atcgcggcct tgccggcgat gggcttcggg
     1801 ccgtggctga cgctgaccgt cgccggattg tgcatgggga tgatgctgtt cctgatggcg
     1861 tcgggcctca cgctgatctt cgggctcatg aacgtcctga acttcgccca tgcggctttc
     1921 gtcacgaccg gcgcctatat cgccgcgtcg gtgctgtggc ggctcacgga ctggacgatg
     1981 gagccgtcgc tcgccctcaa cctcgcggca ttggcggtgg cgctggtggt ggcggcggtg
     2041 gcgagcggag cgctgggata tgtgttcgag cgcctcgtca tccgccaagt ctatggggcg
     2101 ccgctgcggc agatcctgat cacggtgggc gcgctggcgg tggtcgagca gctgatcatc
     2161 gtcgtctggg gacccggagc cattccgctg ccgcggccgg cgatgctgcg cggcagcgtc
     2221 ttcatcggcg gttcggcgat cgagacttat cgcctcgtgg cgctcggggt cggactcctg
     2281 gtggcggcgg ccatgcatct cgtgctcacg cacacgcgga tcggcctcct ggtccgggcc
     2341 ggagtcgagg atcgcgagat ggtcgaggcg ctgggctatc gcgtccaccg cctcttcatc
     2401 ggtgtcttca tcgccggcac cgctttggcc ggcatgggcg ggatgatgtg ggggcagtac
     2461 cagacgctgg tgaatagcca gatcggcaac ggcatgacga tactcgtctt catcatcctg
     2521 atcatcggcg gattgggctc ggtcggcggc tgcttcatcg gcgcggtcct ggtcgggctc
     2581 atgagcaact atgtcggctt cctcgcgccc aagctggcgc tcggcagcaa tctcttgctc
     2641 ctggtgccga tcctgctgtg gcgcccgcgc ggcctcttcc ccatcgcgga acactgagga
     2701 ggcgatgagc cagcagccgc gcgccgcgct cgacgaaccc gcaaaggccg ctccggttgc
     2761 cgccggaggc gcgagccgcg tccgttccgt ctcgcgctgg ctcgtctcgg acgacgcgcc
     2821 ggggcgctgg tggctcaacg ccgtcttcct gctgatcctg ctggcgctgc tcgtcgctcc
     2881 gttcctcttt ccgggcacca aggcgctggg cgtcgcgtcg cgcatctgca tcttcgtgat
     2941 cctggtggcg agctacgacc tgatgctggg ctacaccggc atcgtctcct tcgcgcacac
     3001 caccttcttc ggcatcggcg cctattgcgt cgccattctg ctggcgaaag gttgggtcgg
     3061 ctggggggcg gtgattctgg gggcgctcgc cggcgtcctc gtctccttcg ccgtggcggc
     3121 ggtgatcggc atgctcagct tgcgcgtgcg tgcgatcttc ttcgcgatga tcacgcttgc
     3181 ggtcgcgagc ttcgcccaga tcgtcgcatc acagtggcgc gatctcaccg gcggcgagga
     3241 cgggctcacc ttctcgctgc ccggcctgct gatgccgagc tttcgcgtct tcgccgatcc
     3301 cgtgctgggc gtcaccgtga acggcgcggt cgtcacctac tacctgacgc tcgcggtgtc
     3361 ggtggtgctg ctcctgctga tgctgcggat cgtgaactcg cccttcggcc gcgtgctcca
     3421 ggcgatccgc gagaacgagt tccgcgtcga ggccatgggc tatcgggtcg tcgcctatcg
     3481 cagctgggcc tgcgtcctct ccgccggatt cgccgcgctc gccggcgcgc tcatggcgct
     3541 cgagctgcgc tatgtcggcc ccgatgccac gctctccttc tcgctgatgg tcgacatcct
     3601 gctgatgatc gtgatcggcg gcatgggcac gctttacggc gcggtgatcg gggccatcct
     3661 cgtcgtgctc gcgcagtatt acttgcagaa tgtcctgggc atggtgagcg gcgcctttgc
     3721 cgggatgccc gtgctgtcgg cgcttttcca tcccgaccgc tggcttctgt ggctcggact
     3781 cctcttcgtg ctcatcgtca ccttcttccc gcaaggcatc gtcgggcggc tgcgccggct
     3841 ttagccgacg ctctccccgc gagagccgga gaaccaggcg ccggtttttt tagcagcgat
     3901 agccggaaga ggttgcggtc gcggtcgagc cgttggggca gacgacggtg gagccctggg
     3961 gcacgaccac ggtgggcggt gggcaggagc agccgcaggc cgccagcagg aaagcgcaga
     4021 acgcaagaac gggccaagca cgcatggtca tgcctcgaat tggaaatgct actcgggtaa
     4081 caagcgccca tcggcgggcg ttccgttctc atgggtgcgg gctcgcgcga ttgctggcgc
     4141 aatcgctggc gcccgcccgc gacgacgctg tgcgtcgcgc gggtcagccg gcgccgagga
     4201 cgatgcggtc gaggcgcagc cagacgcggt cgagcgcttc tgccatgagc tgggcgtcgc
     4261 gcacggactc cggcatcagc tcgtggcggt cgcgcagcac cagcatggtc accgacagag
     4321 actcctcggc atggcgcact tcgacgccat gggagaagca atcggcgaag tagggttcct
     4381 tgacgatgtt cgggaaattc acggccagcg catcggcgag cgcccgcatc cagtcgtccg
     4441 acatcagctc gacggcgcac cagggcccga gggatcgcgc atagagatgt tgcgccaggc
     4501 ggacgaagtc ggcggtgtcg gaatcgagat gccggcagag cgcgtcggac tcgccgaacg
     4561 cctcgtcttc aagcaggagc ttggcaccga tgttccggaa cgcgcgcgtg agctgataat
     4621 gatgggtgtc gccgccgggc aggccgtcat cgtcgacgaa gatctggagc ttgcgcttgc
     4681 gcaaagcggg gctgtcgaga tgcggcaggt agtagacggc ggcgcgggtc gcatgcatgg
     4741 cggactgata gacgaggtgg atctgaccga gcagcttggg atcgttcgcg gcgcccggcg
     4801 gcaaagcggc gaggtgacgg aagaacggca tgcgcgaggg gcaatgagtc gcggcaatcc
     4861 gcttgatcat cgccggaaat gcttccaccg cctcggccga aatcggcagc gtcgccgtct
     4921 gctcggtttt cgggctggcg aaggttttca tctccggttc tcctgatccc gcatccgcgc
     4981 agacgccgcg gacagaaaga tcgccgaacc gtcgctaata tcgcgttaag ctgcgtcatt
     5041 atttcttcat gtttgagaaa acggtaacca agaccggtgc aatctgcgtc gtccgccacc
     5101 cgccggagat cggagatgtc gtcctctgcc ctgcggcttg gctgcgcctg gctcaccctc
     5161 ttcgtgatcg gcaccgatct cttcgtcgtc tcgccgctgc tgcccagcgt cgccgcggat
     5221 ctcgccgtcg cacccgcgcg gtcgggctgg tgcgtcacgg tattctcgct gacctatatg
     5281 gtgatggcgc ctgcgctcgg gcgcgccgcc gatgcgttcg ggcggcgccg cctgctcctc
     5341 gtggctctcg cgggatttgc cgcggcgaat ctctgctcgg gcctgacacg ggattttgcc
     5401 ggcctgctcg cggcgcgaat tgcggccggc gccgcagccg ccggcgtgac gccgtccatc
     5461 tatgcgctgg tcggcgattg cgcgccggaa gagcggcggg ccacctggtt tgccgtggcg
     5521 gtctccggcc tgttgctgtc gctggcgctc ggcgcgccgc tgggcacgct gctcgcccac
     5581 gccttcggct ggcagagcgt gttcgtggcg gtggcggtcg cgagcagcct gctcctgccg
     5641 ctgaaccgca tggcttggcc gagctcgtcc gctgccgcgc cggcgacgag cggcgacggg
     5701 gaaagtggat ttctcgtcat ggcgctgcgg ctcgcgccga cggcggtgtg gagcacggcg
     5761 ctctacgcgg tctacaccta tctcggcatc gggctggcgg cgtggggcta cgccgccgat
     5821 cggatcgccg gggtggtcct ctgctacggt gccggggccg tcgtcggcgc gctctgcggc
     5881 ggccggctcg ccgaccgttt cggcgcccgg cgtatctgtt tcgtcagcct gggcggactg
     5941 ggcgccgcgc ttcttgcact cggcccggcg cttcgtgccg gcgtgccgat ggagctgctg
     6001 ctggcggtcc tctccgcgct cgctcagctt ttcttcccgg cgcaacaggc ggggctcgcg
     6061 cgcgatttcg cggcggcgcg ggccacggcg atggcgtgga acaacagcgc gctgttcctc
     6121 ggcatcggcg ccggctcgct gatcggcggc gcggtcatgg cgcgcggcga cctcgccagc
     6181 gtggcaacgg tatccgccgt gatcgcgctc gcaggagccg ccctcgttcg cgccgtggcg
     6241 ccccgcccgg cgccggcgcg tccggtgtga acgcggcaaa ggttaacgag atttcagagg
     6301 ttgttaaatt ttgcggtcta gactgaaccg ttcgtcgtct gcgctggcgg caaagcgcac
     6361 cggcccggag cagaggcggg ggcactgcca atgagcttgg atcaccagga cgagcccgca
     6421 gcagcgaagc gacaatccgc ctcgctctcg tcccgagcag agagtttcgc tgaaaagtgg
     6481 cgttcccgct tgacgtctcg tcttttcatg cttgtcctga tcgccgtgct gccggcaatc
     6541 ggcatccagg cctataacga gttcgacctg cgccgctcgc gcgaagcgga catccgccag
     6601 caggtgatcc agatcacgcg gcagttcggc gaggagatgg gcgagctgcg cgagggcgcg
     6661 cgccagctcc tcctggccat gagccagctc cctgtcgcgc agagcggcca tggcgccgaa
     6721 tgcagcaagc tcttcgcgtc gctgcgccgg aaattcccga attacagcct gctcggcgct
     6781 gccgacgtga agggcaacgt cttctgcgcg agccggatgg tcagccattg gaatgtcgca
     6841 gaccaacctt ttttcacgcg cgccatggcc cgcgatggcg tcgcggtcgg gaattatttc
     6901 gtcgattccg agaccaaaga gaaggagatc cacttcgcgc atcgcttcta cgacgcatcg
     6961 ggcgaggtcg ccggcgtggt cttcgccggg ctcgatctcg catggctgtc ggaccattta
     7021 aaggagcgcg ggctttcgcc gacagcgtcg atcctgatcg ccgatcgcga aggcaacatc
     7081 atcgcgcgtc tgcctaaccc cgagaagttc gtcggcaaca acatgcgcgg cggccatgcc
     7141 gagatcatgg acggcgacaa ggccgggtgg gaagaagccg tcggcgtcga cggagagacg
     7201 cggatcttcg gctatgtgcc tgcccaattg ccgccttacg accttttcct gagcgccgga
     7261 cagtcgaaat ccgaggcgct ggcgccgatc gataacgcga cacgccgcgg catcctgctg
     7321 attctgctgg gcctgacgct ggcgtgcttc gccgcctggc gcggcggcaa ggtcttcatc
     7381 cagcgcccga tcggccatct gctcgaggtc acgaaggcgt ggcgcgacgg cgacgagaag
     7441 gcgcgggtcg aaatcaagga caccgagtcg gaactcggcc ggctgggtgc ggctttcaac
     7501 gacatggcga cggcgctcgc cgcgcgtcac gaggatctgc ggcagctcaa tgccacgctc
     7561 gagcagcgtg tcgagcagcg caccaaggaa ctcgccgacg cgcatcgcct cgcggtcctg
     7621 ggcaagctca tcgcgaccgt gagccacgag ctgcgcaatc cattggcagt gatccgcaac
     7681 accaccttcg ccttggcgga ggcggcaaag aagcagggcc tcgacgtcga gcggtccatc
     7741 gagcgcatca accgaaacat cggccgctgc gtcgagatca tcagcgagct cctcaactac
     7801 acgcgttcgc cgcagctcga attcgtcgcg caggatttcg atcgctggct cgtcgggatt
     7861 ctggccgatt acgacatcga agacgggatt tcgctgcgcc acgagttgca cgccggcgtc
     7921 atggtccgct tcgattcggg ccagctgcag cgggtcttcg tcaatctcat cgacaatgcg
     7981 cgcgacgccg ttcacgaagc gaagggcacg gccagggatc cgtcctacgc gccgcgggtc
     8041 agcgtgaaga gccgcgtcga cggccggcgc atcgccatca cggtcgagga caatggcagc
     8101 ggcatcccgg cggagatcct gcccaagatc ttcgagccgc tgttcagcac caagggctcc
     8161 ggagtcggct tgggcttgcc gacggtgaag cagatcatcg accgccatcg cggacagatc
     8221 gaagtcacga gccgcgtggg cgaagggaca agcgtcgcca tctggctgcc gctcgcggtg
     8281 ggccaggaac tggccgcatg agcggcgagg cgttctcgcg cgtactcgtc atcgacgatg
     8341 accgcgatct ggccgaaagc gtggccgagc tgctcgaggc gcgcgggcac aaggtcagcc
     8401 tcgccttcac cggcagagac ggtgtcgacc tggtcgacca gaacgccttc gatctcgtcc
     8461 tgctcgacat cgaaatgccc gagaagagcg gcgtcgacac gttcctcgag atccgcaaaa
     8521 aagggcatga cgttccgatc gcgctgatga ccggcgccag cgtcgagcag catttctatg
     8581 atgcgctcga gagcggcacc ttgtgcctgt tgcggccgcg atcgaccgcc gccgacgtca
     8641 tcgccgcgac ccgcggcaag cagccgcgct gcgtcgtgct ggtgagcaac aacgtgaccg
     8701 cgctcatcgc gcgcatccag ccattcctca gccagcagca ctgcacgacc gtcatcgcgc
     8761 gctcgagcga cgaggggctc aaatgggtgt ccgcgggtct tgcggattgc ctcgtgttcg
     8821 atcagccgct gtcgagcgcc gcgggcctct atatttactt gagcctccgg cggagcggcc
     8881 ccatgtcgcc gaccatcctc gtcacgctcc ccaatgacgc gccgaatgac gcgacgccgg
     8941 caatccaggc catgaccgcc gacatcctga agaagccgtt cgatcccgag gaagtcctga
     9001 gctggctgga gcggcgggcc gagcggacat gagcgaccaa cgatttcccg gcggggctcc
     9061 cggggcgtcc gtcccggatc ggccgcgcgg cgtgcgcccg ctggtggcgg ccatgccctt
     9121 cacgctcatc ggccaagtgg ccatggccgg aatgggcgat cccgatgtcg tgccgctctg
     9181 gtatggcgaa agcgacgtcc cgacgccggc gctcatcgcg gaggcggcgg tcgcttcact
     9241 cagagcgggc gagaccttct ataccttcaa gcgcggcatc cccgaactgc gcggcgcgat
     9301 tgcggcctat ctcgccggat tgtacggccg ggccatcgag gtcgagcggg tgatcgtcac
     9361 gtcgtcggcg atgagcggca tcatgatgct cttccaggcg ctggtggagc cgggcgacaa
     9421 cgtcgtcgtc gtcacgccgc tctggccgaa tgtcgtcgac accgtcacgg ttctgggcgg
     9481 cgagatacgc gcggtgccgc tggtcggttc ccccgacggc agctggcggc tcgatctcgc
     9541 gcgcgtcatg gccgcgtgcg accccaagac gcgcgtcatt ttcgtcaact cgcccggcaa
     9601 tcccacgggc tgggtgatga gaagcggcga agcggcggat ctcctcgcct ttacgcggcg
     9661 gcgcgggatc tggctcgtcg ccgacgaagt ctatgcgcgc ttcgtctatg acggcagccg
     9721 ggccgcgccg tccttgctcg agatcgccga gcccgaagac cgcgtggtgg cggtcaacag
     9781 cttctccaag gcctgggcga tgacgggctg gcggctcggc tggatggtgg cgccgcgcga
     9841 gattcaggac gtcgccgaca agctcgtcga gttcaacacg agctgcgccc cgcctttcct
     9901 gcagcgcgcc ggaatcgttg cgctggagca aggcgaaccg gtggtggcgt cgctgctgga
     9961 gcgctgctcg cgcggccgcg acatcatcat tcgggggctc aaccgctttc cccgcattca
    10021 cgtcgccgca ccgccgggcg cgttctacgc cttttgccgc atcgacgggc tcaccgacag
    10081 cctcgccttc gccaaggaaa acctggcgcg cgtcaaggtg ggcgtggcac ccggcgcggc
    10141 cttcggaccg gcgggcgagg gccatctccg cttctgcttc gcctgcgcgc ccgcacggct
    10201 cgaactggcg ctggagcggc tggcgcccat gctggggtga tgggccgacg tcggatttga
    10261 gcgcggtgcc ttcctacggg ggaatgcgat ccacgcgacg ttgacccgag ccccggcttg
    10321 catgcggctg tcatcacatc cggctgccga agtccgagcg cgctgatcgg atctctccga
    10381 gcggtggtgg aactcatctc agggttgctc atcgcgattg ccaaaacgtc ggctcgggtt
    10441 atatcgtcaa ccctgcgaac ggcctggcac tcttgtaaaa atagaacttt gtggagacca
    10501 gggccgatcc ggggtggagg caccatggtc gctccggcag agagaacggt gatgttcctc
    10561 gatatgcgcg acagcacgcg cctctacgag gcaatcggcg acgtcgccgc cgagcatacg
    10621 ctgaggcgcg ccgtcaacct ctgcgcccgc cagatccagg cggcgcgggg cgccgtggtc
    10681 agaagcacgg gcgacgggct gctggcggtc ttcgacgacg ccgcggcggc gctggcggcg
    10741 gcgcaacaga tccagatcga gctcctgaag cacaagatcg agggcatcgc catcggggtg
    10801 catggcgg
//
LOCUS       DASYNS010000063         3879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2200870, whole genome shotgun sequence.
ACCESSION   DASYNS010000063 DASYNS010000000
VERSION     DASYNS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2200870"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VGP50_02030"
     CDS             complement(<1..345)
                     /locus_tag="VGP50_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEV8026175.1"
                     /translation="MAKTYDIAVIAGDGIGKEVVPEGMRVLEASARRHGFAFRWRDYD
                     WSCERFARTGAMMPKDGLEQLSSADGIFLGAVGYPGVPDHVSLWGLLIPIRRGFRQYV
                     NLRPVRLLAGVQS"
     gene            complement(442..3669)
                     /locus_tag="VGP50_02035"
     CDS             complement(442..3669)
                     /locus_tag="VGP50_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8026176.1"
                     /translation="MDNSLETLIVGAAATIAVFSVASVLYVDIKRRIRLDGAREALVV
                     GIFFGLCAASSMLIPLRLLPGMPVDGAPVAIALAAPFGGGLAIAVAATLAAALRLWLG
                     GAAPLAGIAEVALAALAGLFVNLIAERLQASRARRVLALALVLPSAGYVGLALRWPAV
                     TGHEADAAAIIEMSAFLVLGTLAFGALLLRQMSAEQKLADSDGRFEALAANVPGIVFQ
                     CAMQPSGEFSFSHLSGHLAEFFGTKSEEVETNASNILRMIHPEDLEAFRRSLREAGAD
                     LALWSHDFRMIDPGGGERWVRGRARPRSRPAGSVVWHGAMVEVTRRKQREEAQRRIER
                     HYRLIAETSSDVMQITAADGTLSYVSPSAARLFGYPVAELVGRPADALLHAADVESVR
                     AARHQAGIGGKPVTVSCRLRRKDGAYVAVTETCRPADEADAEGAGETISIIRAASAQT
                     AVTGDERPFARTLEAMGAGVLITDPNAPDNPIVFANTAATIITGYSVADLIGRNLRML
                     QGPDTDSGAADALGRAMAEERPAVVTWFNHRRDGTSFRSRLHMNPIHNARKQLQAFVA
                     VFFDLGGAEAVASAAQAPPPTDDALSSFVSMLTQELRTPLGGVIGFSDLLLEGALAPE
                     QRRYATSVRNAGRSIRAIIDNAIDLAAIDTVPAIAETAFSIAELALSCSSVVWQAARE
                     KKLELNFVMKPDVIDAVRGHPDRIRHALLDLLDDAVRSTDNGAVTLTVARLEDREGKT
                     AVRFAVIDSRAGNAAEGEPEALGAARTMPPICRALVERMGGTAGAAATPAGNVFWFAL
                     ALMPADGAVERPRARRRARILLAEDTAMSQELVLAVLTRAGHEVEVVGDGAAAVEAIR
                     KRAFDLVLLDLQMPQMDGIKAAQAIRGMPGRAKDVPIVALTARASSKDAERCRAAGMN
                     DHVAKPISAGDLVALVDRWLETRPAEQDAPRRRTAARPPLVDKLVHENAVEALEQHLG
                     PDKAAQMVDAAIKEIPERLERMLRQVSDRGRIAEDANELVAVAGNLGFNELRAQCRRL
                     AEINGEADDWQVGVMVENAKTAAERAIAAMRRAERYVPSGMTEIG"
BASE COUNT          642 a         1349 c         1273 g          615 t
ORIGIN      
        1 cgactgcacg ccggcaagga gtcgcaccgg ccgcaggttg acatattggc ggaagccgcg
       61 ccggatcggg atcaaaagcc cccacaggga gacgtgatcg ggaacgcccg ggtaaccgac
      121 ggctccgagg aaaataccgt cggcgctgct aagctgctcg agcccgtcct tgggcatcat
      181 cgcgcccgtt ctggcaaagc gctcacagct ccagtcgtag tcgcgccagc gaaaggcgaa
      241 gccatgccgc cgtgccgacg cctcgagcac gcgcatgcct tcgggcacga cttccttgcc
      301 gatgccgtca ccggcgatga cagcgatgtc ataggttttc gccaaggctc tcctcccttc
      361 gttcgctgga cgatagccga gtgccgacgg ccccgccacg ccttcgccac ccattccttt
      421 cacgaggaga gggctgtggc ctcagccgat ctcggtcatt cccgacggca cgtatcgttc
      481 cgcgcggcgc atggccgcga tcgcccgctc ggcggccgtc ttggcgttct cgaccatgac
      541 gccgacctgc cagtcgtcgg cttcgccgtt gatctccgca aggcgacggc attgcgcccg
      601 aagctcgttg aaaccgagat tgcccgcgac cgccaccagc tcattcgcat cctcggcaat
      661 acgtccgcga tctgacacct gccgcagcat gcgctccagc cgctcgggaa tctctttgat
      721 cgcggcatcg accatttgcg ccgccttgtc gggaccgaga tgttgctcga gcgcctcgac
      781 cgcattctcg tggacaagct tgtcgacgag aggcggccgc gccgcggtgc ggcgccgcgg
      841 cgcatcctgc tcggcgggcc gggtctcgag ccagcgatcg accagcgcca cgaggtcgcc
      901 cgcgctgatc ggctttgcca cgtgatcgtt cattccggcg gcacgacaac gctcggcatc
      961 cttcgaagag gcgcgcgccg tcagagcgac gatgggcacg tccttggcgc gaccgggcat
     1021 gccgcggatc gcctgcgccg ccttgatgcc gtccatctgc ggcatctgca gatccagcaa
     1081 gacgagatcg aaggcgcgct tccggatcgc ctcgacggca gcggcgccat cgccgacgac
     1141 ttcgacctca tgacccgcgc gggtcaggac cgcgaggacg agttcctggc tcatcgccgt
     1201 gtcctcggcc agcaggatgc gcgcccgccg gcgggcccgc ggccgctcga ccgcgccatc
     1261 cgccggcatc agcgccaagg cgaaccagaa tacattgccg gctggcgtcg cggcggcacc
     1321 ggccgtacca cccatgcgct cgaccagggc acggcaaatc ggcggcatgg tgcgcgcagc
     1381 tccgagcgct tccggctcgc cttcagcagc gttgccggcg cggctgtcga tcacggcgaa
     1441 tcggacggcg gttttgccct cccggtcctc gagccgcgcg acggtgagcg tcacggcgcc
     1501 gttgtcggtc gagcgtaccg cgtcgtcgag aagatcgagt agggcatgac gaatgcgatc
     1561 gggatgtccg cgcaccgcgt cgatcacatc cggcttcatg acgaagttga gctcgagctt
     1621 cttttcgcgt gctgcctgcc agacgacact gctgcaactc agcgcgagct cggcaatgct
     1681 gaacgcggtc tccgcgattg ccggcacggt gtcgatcgcc gccaggtcga tggcattgtc
     1741 gatgatggcg cgaatcgaac gcccggcgtt gcgcacggac gtcgcatagc gtcgctgctc
     1801 gggtgccagc gctccctcga gcagcagatc gctgaagccg atcacaccgc cgagcggcgt
     1861 gcgcagctcc tgcgtcaaca tcgagacgaa gctcgacaag gcgtcatcgg tcgggggcgg
     1921 cgcttgcgct gcgctcgcga ccgcctcggc accgcccaga tcgaagaaca cggccacaaa
     1981 ggcctgcagc tgcttgcgcg cgttgtggat gggattcata tgcaggcgcg agcgaaagga
     2041 cgtcccgtcg cgccgatggt tgaaccatgt aacgacggcc ggccgctcct ctgccatggc
     2101 ccggccgagc gcgtccgccg ctccggaatc ggtgtccggc ccttggagca tgcgcaggtt
     2161 ccgtccgatc agatccgcca cgctatagcc ggtgatgatg gtggccgccg tattcgcgaa
     2221 gacgatcggg ttgtccgggg cgttcgggtc ggtgatgagg acgccggcac ccatcgcctc
     2281 gagcgttcgc gcgaagggac gctcgtctcc cgtgaccgcc gtctgggcgg aggcagcgcg
     2341 gatgatgctg atcgtttcgc ccgcgccctc ggcatcggct tcatccgcgg gccgacaggt
     2401 ctcggtaacg gcgacatagg cgccgtcctt gcgccgcagg cgacagctca cggtcacggg
     2461 cttgccgccg atgcccgcct ggtggcgagc ggcgcgcacg gactcgacat cggcggcgtg
     2521 cagcagcgca tcggcgggcc ggccaacgag ctccgccacc ggatagccga agagccgcgc
     2581 cgccgaaggc gacacatagc ttagcgtgcc gtcggccgcc gtgatctgca tgacgtcgct
     2641 cgaggtctcg gcgatcagcc ggtagtgtct ctcgatgcgg cgctgcgcct cctcgcgctg
     2701 cttgcgccgg gtcacttcga ccatcgcgcc gtgccagacg acgctgccgg caggccgtga
     2761 ccgtggccgc gcgcggccgc ggacccagcg ctcgccgccg ccgggatcga tcatgcggaa
     2821 atcatgggac caaagtgcca gatccgcgcc agcctcgcgc agcgagcgcc ggaacgcctc
     2881 gagatcctcc ggatggatca tgcggagaat gttgctggca ttggtctcga cctcctcgga
     2941 tttcgtgccg aagaactccg ccaggtgccc gctcagatgt gagaaggaga attctccgga
     3001 gggctgcatc gcgcattgga agacaatgcc cggcacgttc gccgccagcg cttcgaagcg
     3061 cccgtcgctg tcggcgagct tctgctccgc cgacatctgg cgaagcagca gcgcgccgaa
     3121 cgccaaggtg ccgagcacga ggaacgccga catctcgatg atcgccgcgg cgtccgcctc
     3181 atggccggtg accgcaggcc agcgcagggc aaggccgaca taaccggcgc tcggcagcac
     3241 gagcgccagc gcaaggactc gacgggcgcg gctcgcctgc agccgctcgg caatcaggtt
     3301 cacgaacagc cccgccagag cggcaagcgc aacctctgcg atcccggcga gcggagcggc
     3361 accgccgagc cagagacgga gcgccgcagc cagcgtcgcg gcaacggcga tggcgagccc
     3421 gccgccaaag ggcgcagcca gcgcgatcgc gaccggagct ccgtcgacgg gcatgccggg
     3481 cagcaagcgg agcgggatca gcatggacga tgccgcacac agtccgaaga atatgccgac
     3541 gaccagcgcc tcgcgcgcgc cgtccaggcg aatccggcgc ttgatgtcga catagaggac
     3601 gctcgcgacg ctgaagaccg caattgttgc ggcagcaccg acgatcaatg tctccaatga
     3661 attgtccatt gcgcggcttt tccggggcgg gtgtgaattt tcttgagacg gcctcggatg
     3721 ccgagcccga ccacccttga atagatgctt taaccgcgta gatcaacgcc cctgtgtgcg
     3781 cgtcttttgc gcaacccgcc gttgcgcgca cccatgccct tctgtcgcgg agctttccgg
     3841 tgcggtcttg ctatctagag cgaaatcgtc ccttcaaga
//
LOCUS       DASYNS010000064         1890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2668601, whole genome shotgun sequence.
ACCESSION   DASYNS010000064 DASYNS010000000
VERSION     DASYNS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2668601"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="pspF"
                     /locus_tag="VGP50_02040"
     CDS             complement(<1..1019)
                     /gene="pspF"
                     /locus_tag="VGP50_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015073006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HEV8026177.1"
                     /translation="MAISANAQPILPPLIGESPAFRACLAQVSQLAALDRPVLVVGER
                     GTGKELVAARLTYLSPRWDRPFVKLNCAALAESLLDSELFGHEAGAFTGAVRRRLSRF
                     EAADQGTLFLDEIANASLAVQEKILRVIEYGSFERVGGNDVLSVDVRVVAATNVDLPS
                     LAASGRFRSDLLDRLAFDVVTVPPLRGRPEDIPLLAEHFGRAMARELGRDGFAGFTAA
                     ASAQLAAHAWPGNVRELKNAVERSVHRWPDASARIAEIILDPFASPHRPPAPSAAAPA
                     PSLPPASADADADVAFLARVRAFEEKLLAEALEASHFNQRRTAAALGLSYHQLRHYLR
                     KYRLV"
     gene            complement(1031..1201)
                     /locus_tag="VGP50_02045"
     CDS             complement(1031..1201)
                     /locus_tag="VGP50_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026178.1"
                     /translation="MAILLRRSRVGVLFFSKACAVPTARLDRKPLIRNTFLPIIAIGG
                     GFCYDEKRHYMA"
     gene            1204..1890
                     /gene="pspA"
                     /locus_tag="VGP50_02050"
     CDS             1204..1890
                     /gene="pspA"
                     /locus_tag="VGP50_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HEV8026179.1"
                     /translation="MGIFSRLSDIVNSNINAILDRAEDPEKIIRLIIQEMEDTLVEVR
                     TSAVRAIAEKKEMERRAETLRRERDEWQRRAELAVTKGREDLAKGALLAKTRVVEALE
                     ALEKQIQQIEAGLAKQNEDVGKLQGKLADAKRREKSIVARQKTAKNRLKLRTHLYDER
                     VTDAFARFEQVERALDEIEGKVEAFDLGRKKTLADEFAGMEAEDGVEEELKALKERLG
                     NSIRPTPAKG"
BASE COUNT          366 a          590 c          672 g          262 t
ORIGIN      
        1 tcgaccaggc ggtatttccg caggtagtga cgcagctggt gatagctgag gccgagcgcg
       61 gcggcggtgc ggcgctggtt gaaatgactg gcctcgagcg cctccgccag cagcttttcc
      121 tcgaaagcgc gaacacgggc caggaacgca acatccgcgt cggcgtcggc tgaggcgggt
      181 ggcagcgacg gcgcgggcgc tgccgcgctc ggcgccggcg ggcgatgcgg cgaggcgaag
      241 ggatcgagaa tgatttcggc gatcctggcg ctcgcatcgg gccagcgatg gacgctgcgc
      301 tcgaccgcat tcttgagctc gcgcacattg ccgggccagg cgtgggcggc aagctgcgcc
      361 gacgcggcgg ccgtgaatcc ggcaaagccg tcgcggccga gttcgcgggc catggcgcgg
      421 ccgaaatgct cggcgagaag tggaatgtct tcgggacgcc cgcgcagcgg cggcacggtg
      481 acgacgtcga atgcaagccg gtcgaggagg tcgctgcgga agcggccgct cgcggcgagg
      541 ctcggcaggt cgacattggt ggcggcgacg acgcggacat cgacgctcag cacgtcgttg
      601 ccgccgacgc gctcgaagct gccatattcg atgacgcgca ggattttctc ctgcaccgcc
      661 agcgaagcgt tggcgatctc gtcgaggaag agcgtgccct gatcggcggc ctcgaagcgc
      721 gagaggcgcc ggcggaccgc gccggtaaac gcgccggcct catgcccgaa gagctcgctg
      781 tcgagcaggc tctcggccag cgccgcgcaa ttgagcttga cgaagggacg atcccaacgc
      841 ggcgagagat aggtgagccg cgccgcgacc agctccttgc ccgtgccgcg ctcccccacc
      901 accagcacgg gccggtcgag cgcggcaagc tgcgagacct gcgccaggca ggcgcgaaag
      961 gccggggact cgccgatcag cggcggcaag atcggctggg cgttggcaga tatcgccatt
     1021 attctccatt ttacgccata taatggcgct tttcgtcata gcagaaaccg ccgccgatcg
     1081 caataatcgg caggaacgtg tttcgaatca atggcttgcg atccaggcgc gcggttggca
     1141 cggcgcatgc ttttgagaag aagagaaccc cgacccggct tcgtctcaag aggatcgcca
     1201 cccatgggta tcttttcgcg cctctccgac atcgtgaact ccaacatcaa cgccatcctc
     1261 gatcgcgccg aggacccgga gaagatcatc cgcctgatca ttcaggagat ggaggacacg
     1321 ctcgtcgagg tccgcacatc ggcggtgcgg gccatcgccg agaagaagga gatggagcgc
     1381 cgggccgaaa cgctgcggcg cgagcgcgac gaatggcagc gccgcgccga gctcgccgtc
     1441 accaaggggc gcgaggatct cgccaagggc gcgctcctgg cgaagacgcg cgtcgtcgag
     1501 gccctcgagg cgctcgagaa gcagatccag cagatcgagg ctgggctcgc caagcagaat
     1561 gaggacgtgg gcaagctcca gggcaagctc gccgacgcca agcggcgcga gaaatcgatc
     1621 gtggcgcggc agaagacggc gaaaaaccgc ctcaagctgc gcacgcatct ctatgacgag
     1681 cgcgtcaccg acgccttcgc ccgcttcgag caggtggagc gcgcgctcga cgagatcgag
     1741 ggcaaggtcg aggccttcga tctcggacgc aagaaaacgc tggccgacga attcgccggg
     1801 atggaggcgg aagatggcgt cgaggaagag ctgaaggcgt tgaaggagcg actcggcaac
     1861 agcatccgtc cgaccccggc gaagggatag
//
LOCUS       DASYNS010000065         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5477413, whole genome shotgun sequence.
ACCESSION   DASYNS010000065 DASYNS010000000
VERSION     DASYNS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5477413"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VGP50_02055"
     CDS             <1..310
                     /locus_tag="VGP50_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026180.1"
                     /translation="AERWDVARKIFEHAGRRRRHSARTAIEVVDFIVRRHAASISQRV
                     THALDRSCPDKSIILLLTGGARSGSNAPSVPRRMQKETRTMQDTIVVGVSAFSIAASR
                     "
     gene            428..2482
                     /locus_tag="VGP50_02060"
     CDS             428..2482
                     /locus_tag="VGP50_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8026181.1"
                     /translation="MTMGSTAAIAAYVHAMMGAFEPGSAPGWKLSEWLSESGHGVGVD
                     FAAATRLTSPSDRQHEPPAKDLVLAPASWEQLRAALAATIATPATCDIAAANVAAFAQ
                     AVGLDGIDFDLFRLVFHSHHDAAFGMLCGDLVSTRRIDTLGLCAIALGHAPAELMSRL
                     RRGPLRALNLVSSIRYNVGRFALFVPQRIVDALLPPSDGLADIEEQLIGAPLRPQLAL
                     DDFAHVAAVRDFVLRLLAGAVQGKHRGINVLLHGLPGTGKSEFCKMAAAHLGCDLFAV
                     GEADEFGDEPSRSDRIDALRLAEKLAGRRGNVLLLFDEMEDILQHGEESRAIGRRVRR
                     SGSKVFFNRLLEENVVPVLWTANALDEFDPAFLRRMTFVLEMKMLPLAARARLWEGLA
                     QRQGLSLTREAAAGLARRLKVAPSLMSGAARSVALAGGTAGDIDFVVRALAQPLGPIG
                     REDAGGARFEPALTNAESDLADLEAALMRQGTPRDFSLCLHGPPGTGKSAFARHLAGA
                     IGLTPLVKRGSDLLSRWVGGTEQRIAEAFDEARRDEHFLIIDEVEAFLWSRDGAQRSW
                     EVSMVNELLVAMETHPLPFACTTNHLAMIDAAALRRFTFKVKFDFMTAAQSAAAYRRF
                     FGREPPPALREVAALTPGDFAAAAKQLRFLDAREPSDASILALLEREVAAKNLPTRRI
                     GF"
     gene            complement(2523..>2706)
                     /locus_tag="VGP50_02065"
     CDS             complement(2523..>2706)
                     /locus_tag="VGP50_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV8026182.1"
                     /translation="RVRVTYRDLSGTERVEEAGGLLAICHQHEIDQLDGIFWIQRLSR
                     LRRDRLLKRYEKKQRS"
BASE COUNT          422 a          921 c          888 g          475 t
ORIGIN      
        1 cgcagagcgc tgggatgtcg cgcggaagat tttcgagcac gccgggcgga gacgccgtca
       61 ttctgcccgg acggcgatag aagtcgtgga tttcatcgtg cgtcgtcatg ccgccagtat
      121 ctcgcagcgc gtgacgcatg cgcttgatcg gtcttgcccc gacaaatcga tcattctcct
      181 tttgacaggc ggcgcgcgtt ctggtagcaa cgcgccttcc gttccaagac ggatgcagaa
      241 agagacacgg accatgcaag acaccatcgt ggttggcgtg tcggccttct cgatcgctgc
      301 gtcgcgatag tcgcgcggtc gggttcggcc ggccgcttca caaaacactg atgtgagcca
      361 gggacgaccg cttcctgcgg tcgccgacgc ctgcgcgcat cggcgcgaca gcaaaagagc
      421 gacgaaaatg accatgggat cgacggcggc aatcgccgcc tatgttcacg ccatgatggg
      481 tgctttcgag ccgggctccg cgcccggctg gaagctttcc gaatggctca gcgaaagcgg
      541 gcacggcgtc ggcgtcgatt ttgccgcggc gacgcgcctg acctcaccgt cggaccggca
      601 gcacgagcca ccggccaagg acctcgtgtt ggcgccggcc agctgggagc agcttcgtgc
      661 cgcgctcgct gcgacgatag cgacgccggc gacgtgcgac atcgcggcgg cgaatgtcgc
      721 cgcatttgcg caagcggtcg gcctcgacgg aattgatttc gacctcttcc gcttggtctt
      781 ccatagccat cacgatgccg ccttcgggat gctttgcggg gatctcgtgt cgacgcggcg
      841 catcgacacg ctcgggctct gcgccattgc tttgggtcac gcgcccgcgg agctcatgtc
      901 ccggctccgt cgcggcccgc ttcgtgcgct caacctggtg agcagcatcc gctacaacgt
      961 cggccgcttt gcccttttcg tgccgcagcg cattgtcgac gccctgctgc cgccctctga
     1021 cggcctcgcc gatatcgagg agcagctgat cggcgcgccg ctccgcccgc agctcgcgct
     1081 cgatgatttt gcccatgtgg cagcggttcg cgacttcgtg cttcgccttc tcgccggcgc
     1141 cgtacagggc aagcacaggg gcatcaacgt gctgcttcac ggcctgcccg gtacgggcaa
     1201 gtcggaattc tgcaagatgg ccgccgccca tctcggctgc gatctcttcg ccgtcggcga
     1261 agcggacgag tttggcgacg aaccttcgcg cagcgaccgg atcgacgccc tcaggctcgc
     1321 ggaaaagctc gcgggacgcc gcggcaacgt gctcctgctc ttcgacgaaa tggaagacat
     1381 cctgcaacac ggcgaggaga gccgggcgat cggccggcgc gtgcggcgct ccggttccaa
     1441 agtcttcttc aaccggcttc tcgaagagaa tgtcgtgccg gtgctgtgga ccgccaatgc
     1501 gctcgacgag ttcgatccgg cattcctgcg ccgcatgacc tttgtcttgg agatgaagat
     1561 gctccctttg gcggcacgcg cgcgcctctg ggagggactg gcgcagcgcc agggcttgtc
     1621 cctcacgcgc gaagccgccg ccggactggc gcggcggctc aaggtggcgc ccagcctgat
     1681 gagcggcgcc gcgcggagcg tggcgctggc cggcggcact gcgggcgaca tcgacttcgt
     1741 cgtgagagcg ctggcgcagc cgctcgggcc catcggccgc gaggatgccg gcggcgcccg
     1801 cttcgagccg gcgctcacca acgccgagag cgacctcgcc gacctcgaag cggcgctgat
     1861 gcggcaagga acgccgcgcg atttctcgct gtgccttcac ggaccgcccg gcaccggcaa
     1921 aagcgccttc gcccggcatc tcgccggcgc catcgggctc acgccgctgg tcaagcgggg
     1981 ctccgacctc ctgtcgcgct gggtcggcgg gaccgagcag cgcattgccg aagcgttcga
     2041 cgaagcgcgc cgcgacgagc atttcctgat catcgacgag gtcgaggcct tcctgtggag
     2101 ccgcgacggc gcgcagcgct cctgggaagt ctcgatggtg aacgagctgc tggtggcgat
     2161 ggagacgcat cctctgcctt tcgcctgcac caccaatcat ctcgccatga tcgatgcggc
     2221 ggcgctccgg cgcttcacct tcaaggtaaa gttcgacttc atgacggccg cgcagagcgc
     2281 tgcggcgtat cgccggttct tcggacgcga gccgccgccc gccttgcgcg aggtcgccgc
     2341 gctcacgccc ggcgacttcg ccgctgcggc gaagcaactg cgcttcctcg acgcgcgcga
     2401 gccaagcgac gcgtcgattc tcgcgctgct ggagcgcgaa gtggcggcga agaacttgcc
     2461 cacgcgccgc atcggcttct gagcaagacc gaaaagccgt gcgtcgtgcc actgtcggcg
     2521 gctcacgaac gctgcttctt ttcatatcgc ttgagcaagc ggtctcgccg cagccgcgac
     2581 agcctttgaa tccagaagat gccgtcaagc tgatcgatct cgtgctgatg gcatatggcg
     2641 aggagcccac ccgcctcctc gacgcgttcg gtgccgctca gatcgcgata ggtcacgcgc
     2701 acgcgg
//
LOCUS       DASYNS010000066        17303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_797135, whole genome shotgun sequence.
ACCESSION   DASYNS010000066 DASYNS010000000
VERSION     DASYNS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 17303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17303
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797135"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1154)
                     /locus_tag="VGP50_02070"
     CDS             complement(117..1154)
                     /locus_tag="VGP50_02070"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEV8026183.1"
                     /translation="MNTSADHRPLELPIIDIGCFLTEGSASDRIGVARAIEAACRDTG
                     FFYLTGHAIPGESFQSLEKASRRFFALPAKDKLAIEMARGGSAWRGYFPVGGELTSGK
                     PDLKEGVYFGTELPADHPRVTAAWPLHGVNLWPEQIPELRPAVLGYMEAAVRAGHALL
                     QAVGMSLGLEPQYFARTYTAEPTILFRIFHYPAVTPDEQSRTGSWGVGEHTDYGLLTL
                     LAQDDNGGLQVRSTRGWIEAPPIAGALVCNIGDMLDRLTGGWYRSTPHRVRNTSGRDR
                     LSFPLFFDPDFAAEIKPLPRQSRLDAARSAAETAERWDGVSVHRFSGTYGDYLLSKVA
                     KVFPHLSHKVL"
     gene            1327..2475
                     /locus_tag="VGP50_02075"
     CDS             1327..2475
                     /locus_tag="VGP50_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HEV8026184.1"
                     /translation="MNLRHPASCFCCGDALHRVFDRRRFLLAAAGGAASLALPRRSAF
                     AETVPTIPYDSVPEPLHLPRNLYFGEVSGVAVNSKGHVFALSRGNSTGPAYGAAATQL
                     LEFSRDGKFIGEIGHNLYAWSFAHTVKVDRHDNIWVTDKGSDMVIKFTPEGRVAMVFG
                     RKQEASDEETGPLKHPNPPLPAEDGRFRQVTDVAFDAADNTYISDGYINSRVAKVDKD
                     GNWLKSWGDRGTGPGQFHTPHSIATDAKGNVYIADRSNRRIQVFDGEGNFQRQFTIDL
                     PVPADARPAIGNMPNEAMIAGGTFAPGSPWAICISPGPNPVLYSSDAFPGRIYRLSLD
                     GKVLGVLGQAGKQLKQFGWIHQMACPAPNLLYVAELLNWRVQKLVLHG"
     gene            complement(2499..2960)
                     /locus_tag="VGP50_02080"
     CDS             complement(2499..2960)
                     /locus_tag="VGP50_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV8026185.1"
                     /translation="MTTLASLVAQATQLLNEGLVAPMKDFGLSPTEERVLVALTKRNG
                     RRMFELAEYAVVKQATLSKAVDCLERKRLVERVKVDKDRRCCLVLLTGRGRKIAARLI
                     WRARWQLSALYHSLGAADTQRLETALSHLNYVLDGIARGEAPRKSAAGSNV"
     gene            complement(3194..5179)
                     /locus_tag="VGP50_02085"
     CDS             complement(3194..5179)
                     /locus_tag="VGP50_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8026186.1"
                     /translation="MRRGAKVLRDVIDTIPALVFSTGSAPGDAWVNQRWVEYSGLSVE
                     ETKGLGWQSTIHPDDLDVHVAKWHRSLAGGEPFENEARHRSAKGDYRWFLVRAVPLRD
                     EQGRIVRWYGTLTDVEDRKRTDALLAGERRILEMVARGDTLSRILDSVCRVVEEHAPD
                     VLASILLVEDGRVKHGGAPSLPKAYVDAIEGAAIGPSAGSCGAAAWHGKQVVVSDIAN
                     DPLWTDYRDLALPHSLRACWSTPIMSAEGKVIGTFAMYYREPRSPCPRDQEIIEQITH
                     LAGIAIERKLTEEKLRRSEAYLGEAQKLTHTGSWAADPATRKLLYGSEELLRIFGFDP
                     QQGLPTRDALMERIHPEDRQAVFARVQKAVREKSHYIGEFRVLLPDGAVKYVEGTGHP
                     VFNTRGEITAFVGTTVDVTERKRAEQERERLRQLEAELAHMNRMTMMSALTASLAHEL
                     NQPIAAAITNANACVRWLTRPEPDLEEARAAAMRIAKDGSRAAEIIDRLRSFYKKGGP
                     PRRELVHVNDIAGEMMALLRQEADRHAIAMRSNFAPEVPKVVADPVQLQQVLMNLMLN
                     GIEAMHETGGELIIKSDVVEDGQLLISVSDTGVGLPAGKAEQVFNAFFTTKPQGTGMG
                     LAISRSIIESHGGRLWTADNTGRGATFYFTLPRETAG"
     gene            5336..6247
                     /locus_tag="VGP50_02090"
     CDS             5336..6247
                     /locus_tag="VGP50_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026187.1"
                     /translation="MREAGIDLGPDEIAIGLIEHARDRLPDQFAHAIIELLRGRGVDR
                     QERTRRIENEVHRRIALEGGSPLLLDLLAGMLGPLAVGDVLVARQDRCRRAALVALQR
                     PTACHDDLAAIPPRLHELAFPPAATHDVALDLLEGPREGRLQQRVDDFADRLLRRPSV
                     ERLGGAIPGADDSVHIAHENAVMHEVQQVGLRPQNRFGPPRVGLTRDANGFRVRRGGR
                     AKKRVLVIPGHDGSPPKGSSAAYRAAPPPHKVPAASFGRASASHFGAMRLSRQAAWRD
                     AFPVARDSAGTLDSPHRPSYGKTGALR"
     gene            6259..7416
                     /locus_tag="VGP50_02095"
     CDS             6259..7416
                     /locus_tag="VGP50_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV8026188.1"
                     /translation="MAVIGAGMAGLASAAALQRVGINVTVYEQARRFARLGAGIQIGC
                     NAMQVLRAFGLEPALRAAAFYPRSWNNRDYDTGDTRFDMIFGEAAEQRYGTPYLLAHR
                     GDLHAALASAVNEEDLRLGHQLTSLVSRAEGGIRLGFANGTVAEVDAVIGADGVHSLV
                     KTVLFGEDRANITGRVAYRTVFPSALLQGYPVDDCTKWWGPDRHIVIYYVKPDRSEIY
                     FVTSQPEPEFSVESWSAMGNVDELRAAFDGFHPQVRHVLAACPAVHKWALVDRDPLPR
                     WCEGNVTLLGDACHPMTPYMAQGAAMAIEDAAVLSRCLHGVDRDGIAAAFRRFEASRK
                     PRTSRVQLSSRTNTWLRDPTDPNWVYGYDALTAPLAELAPSSNAARRQPTL"
     gene            complement(7509..7700)
                     /locus_tag="VGP50_02100"
     CDS             complement(7509..7700)
                     /locus_tag="VGP50_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026189.1"
                     /translation="MRLAVPVLLMLASLSGCASVEDLHARDEATCASNGLQPDSRDFN
                     ACVEEQQMLRRAFMSHPGH"
     gene            8024..8821
                     /locus_tag="VGP50_02105"
     CDS             8024..8821
                     /locus_tag="VGP50_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026190.1"
                     /translation="MTVSGINNLPNPWLLQQAQQAAAASAAAAAAATPPGGQTTQGAG
                     APASNAAVNQFSAMIQAMVTQLQSDASSAGSATAPAAAGAAAGQTCANATTASATASG
                     TTSAAASGQTATAANTASNGQNSQVDGHSHGHHHHHGGGADETQKGLSATNQTTLQTD
                     ANNLVDALFGAQSSANAAGTTATAGTTATTAAATTAAATSAATTSTAAAAMGASTLAA
                     STTTAQPGGTTPQTLAAVFAQDMLNAIQAYTAQNPTGITASALNAIA"
     gene            8929..10809
                     /gene="cysC"
                     /locus_tag="VGP50_02110"
     CDS             8929..10809
                     /gene="cysC"
                     /locus_tag="VGP50_02110"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEV8026191.1"
                     /translation="MTKPSRTGRDLPIVIVGQVDHGKSTLIGRLLHDTGSLPDGKVAA
                     IEEASRRRGMPFEWSFVMDAFKAERDQGITIDTTQIRFRSDRRRYIIIDAPGHAEFLR
                     NMLTGAAAAEAAILVVDVVEGMSEQTRRHAFLLKLLGLREVVVVVNKMDRCGYDRTAF
                     NGLAAIVSAYLAELGLAPAAIIPISARDGDGIARRSAPLGWYAGPTMIEALDALEGFP
                     SAADRPLRFPVQDIYKFDDRRIIVGRIDAGRLRVGDRLCFSPGERTARIASIEGWNAP
                     PTVTAAAGQSIGLTLDEEIFVERGQVASAEGAAPPIARRLKLRIFHFGSQPLAVEDEV
                     KVQIGLAERVATVEAIERVVDVNDLTGRPAAAVRRNEVADLVVLCRTPLAVDEGADAG
                     HLGRGVLRRGHDIVASVLVEAALDARQPAASRRPMTPVRSSVSAGERAAAWGHPGAVL
                     WLTGFSGAGKSTLAQALEKELFHAGWRTMLLDADTLRHTLNADLGFSEAERAENVRRI
                     GAVAQLVAESGVVAIVACIAPRRIYRDRLRSELGARYHEIHVKAALAVCERRDVERSH
                     AKAKRGAVAAFTGLSDPYEAPAAPDLEVSTDVLTPRECVDQLLDYVERTLRPADTRQL
                     AS"
     gene            complement(10875..11486)
                     /locus_tag="VGP50_02115"
     CDS             complement(10875..11486)
                     /locus_tag="VGP50_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026192.1"
                     /translation="MHSLRYLVAVIGTLLTVSALAQAPAGPPTRVRGTVAALDGETLT
                     VKSRSGEELSIAFPPGTMVVAMTKRTLADVKSGDFVGVTSVRGGDGKMRALEVHIFPE
                     AMRGTGEGEYAWDLVPNSTMTNGAITGISSATSDNTITVNHQGEMSEIMVPATTPVVA
                     YLPGDVNLLKPGATVLVLVQKKADGGLVAMRVNAEKDGVKPPM"
     gene            complement(11687..13429)
                     /locus_tag="VGP50_02120"
     CDS             complement(11687..13429)
                     /locus_tag="VGP50_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026193.1"
                     /translation="MRATVDKQAARLDRDSEIEELRARLREAEETLQALREGQVDAVV
                     VNGEAGPQVYALENADRPYRVIVERMQEGALTLSPDGAVLYANRSLGRLLRLPGEAII
                     GRRFHDFVATADQGVLTRLVAESEHGGGRGELELRAGDGTGIPVYLSVVDLPDQGQRV
                     ISGMVTDLTLQRQRTHELAESNAKLVAALAQRERAEGQLRQAQKMEAVGQLTAGVAHD
                     FNNLLTVIAGNLELIQARATEVRLRDQVETIQRAIHRGVLLTDQLLAFSRQRALRPRA
                     IPINALLRDTEPLLRRAVGDEVRVLLALGEGVGCCLIDPAELQASMLNLALNAKDAMP
                     RGGALTVATAEAEIADMPDRTPDLPPAGRYVMVAVTDTGHGMPAEIRERAFDPFFTTK
                     EVGKGTGLGLSQVYGFMRQSGGHVTIESAVGAGTTVRLYLPRTDALGASEAWRQAAAA
                     VSASAPAARKVLVVEDDEDVRTFAIELLHDIGYIAIEAESGPAALRLLESGVAVDVVF
                     SDVRMPDGMSGFQLAHEIRRRLPGTPVVLTSGVAPASDVVGDVPILRKPFRRDDILQA
                     IEAALKRGAATTEA"
     gene            complement(13432..13752)
                     /locus_tag="VGP50_02125"
     CDS             complement(13432..13752)
                     /locus_tag="VGP50_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEV8026194.1"
                     /translation="MTERGPSAGNDERYVLRLYVTGSTARSARAIENMRQICDGHLRG
                     RHDLEVVDLYEHPEAAAEENVFAAPTLVKLRPAPLRRVIGDLSDRARVLVSLGLGSKA
                     AAGA"
     gene            complement(13749..14039)
                     /locus_tag="VGP50_02130"
     CDS             complement(13749..14039)
                     /locus_tag="VGP50_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEV8026195.1"
                     /translation="MSLEHYNLRLYVAGQTPKSLAAIANLKRICETHLAGRYAVEVID
                     LIDKPQLAAMDQILAIPTLVRRVPEPLRRIIGDLSQTERVLVGLDIELRRNN"
     gene            complement(14036..15829)
                     /gene="kaiC"
                     /locus_tag="VGP50_02135"
     CDS             complement(14036..15829)
                     /gene="kaiC"
                     /locus_tag="VGP50_02135"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEV8026196.1"
                     /translation="MAMRGGEAERPKKIEHGLSQLSKAPTGIEGLDDILLGGLPAGRP
                     TLICGGPGCGKTLLSMIFLVNGATRFDEPGVFVSFEETGKDLADNVASLGFDVPGLIA
                     AKKLAIDHVHIERSEIEESGEYDLEGLFVRLGHAVDKIGAKRVVLDTLETLFMSLSNT
                     AILRAELRRLFAWLKVRGLTAIITGERGEGALTRHGLEEYVSDCVILLENRVEEQVTT
                     RRLRIVKYRGSAHGMNEYPFLLDDQGITVVPLSSAGRVGAVSREAVSTGVAGLDAMLG
                     VGGVYRGSSVLISGVAGSGKTILGASAAAAACARGERCIFFSFEESAAQLVRNMASTG
                     IMLQPHVDAGRLRFETTRPTAYGFEMHLARMHRELDAFQPAIVIVDPVTGFRGSETEV
                     HALLLRMLDVLKSRGITTVFTSLTTSDERAAQSDFGLSSLMDTWISLIDIESNGERNR
                     GLYVLKSRGMSHSNQISEYALTREGVRMIEPYLGAAGVLTGSARVAQEARDQAEAQRR
                     RETVERQRAQLQRKRALAERQIAELRAEIEAEEAEVARLIAQEEGQETLREESRSAIA
                     VSRGVKPNGEDAARRPKSRRRSDTAEHMRTR"
     gene            16207..>17303
                     /locus_tag="VGP50_02140"
     CDS             16207..>17303
                     /locus_tag="VGP50_02140"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEV8026197.1"
                     /translation="MAQRPARPRTPRSTRTQARGASGQGLASLPVSIEIEPMEAKLVD
                     ALPDGAGWQFEPKWDGFRCLAARAGDAIDLRAKSGKPLGRYFPDMVALLRALPVERFL
                     LDGELAIQIGDTLAFDALQARLHPAESRVRKLAAETPAILILFDVLAIDGESLLASTL
                     SARRSVLESFYAEAGERKRLKLSPWTDDRAEAQRWLERSGCALDGVIAKRRDDIYRPG
                     ERAMLKVKRRRSADCVVGGFRYASGGAEVGSLLLGLYDDAGKLNHVGFTSTIHDVERH
                     LLTRHLEQLIEKPGFTGRAPGGPSRWSSERSAEWQPLRPRLVVEVRYDQVTADRFRHG
                     TKLLRWRPDKAPRQCTVDQLEREAAPSALIER"
BASE COUNT         2889 a         5945 c         5665 g         2804 t
ORIGIN      
        1 gtgtcctccg gcagttccga acgacgcaac cggcgaagac gggcaaccgg ggccgaggga
       61 atggcgttgc ctggacgggg cgtgatgcgc ttgcgctgaa tcggcaaagc ctgagctcag
      121 aggactttgt ggctgagatg cggaaacact ttcgcgactt tggacaagag atagtcgccg
      181 taagtgcccg agaagcgatg gacgctgacg ccgtcccaac gctcggccgt ctccgccgcg
      241 gaccgggcag cgtccaggcg gctctgtcgc ggcaacggtt ttatctcggc ggcaaaatcg
      301 ggatcgaaga acagcgggaa ggagaggcga tcccggccgc tcgtgttgcg aacgcggtgg
      361 ggcgttgagc ggtaccagcc gccggtcaga cggtcgagca tgtcgccgat attgcagacg
      421 agagcgcccg cgatcggcgg cgcctcgatc cagccgcggg tcgagcgcac ctgaagcccg
      481 ccattgtcgt cttgcgcgag aagcgtcagc aggccgtaat ccgtgtgttc gccgacgccc
      541 caggatccgg ttcgcgattg ctcatcgggc gtgaccgccg gatagtgaaa gatgcggaag
      601 aggatcgtcg gctcggcggt gtaggtgcgt gcgaaatact ggggctcgag gccaagactc
      661 atcccgactg cctggagcag cgcatgcccg gcgcgaaccg ccgcctccat gtatccgagc
      721 acggcagggc ggagctcggg tatttgctcc ggccagagat tgacgccgtg gaggggccag
      781 gcggccgtga cgcgcgggtg atcggctggg agctctgtgc cgaaatagac gccttccttg
      841 agatccggtt ttcccgaggt gagctctccg ccgacgggaa agtagccgcg ccacgcggat
      901 ccgccgcgcg ccatctcgat cgcaagcttg tccttcgccg ggagggcaaa gaagcggcgg
      961 ctggccttct ccaagctttg aaagctctca ccgggtatgg cgtgaccggt tagatagaag
     1021 aagccggtat cgcggcaggc cgcctcgatc gcccgtgcca cgccgatccg atccgaggcg
     1081 ctcccctcgg tcagaaagca accgatgtcg atgatcggca attcaagcgg acgatgatcg
     1141 gcagaggtgt tcatgagagc attatatagc gcggcggagc gcctcgcggc cccatcggca
     1201 cccaagaccg cacctgtttg ccgcgaaacc cgttccgact agactgcgcg ccgcccattc
     1261 cggttgccgt cgaaacggcc ggcgcttgcc gcaatccggg ggggcggggg ggggggggaa
     1321 agcgacatga accttcgaca tccggcgtcc tgcttctgct gcggcgatgc gctgcaccgc
     1381 gtcttcgacc gtcgccgctt cctgctggcc gcggccggcg gcgcagcctc gcttgcgctg
     1441 ccgcggcgtt ccgccttcgc cgagacggtc ccgaccatcc cttacgactc cgttccggag
     1501 ccgctgcacc tcccgcgcaa cctctatttc ggcgaggtct ccggcgtcgc cgtcaattcc
     1561 aaaggccatg ttttcgcgtt gtcgcgcggc aacagcaccg gccccgccta tggcgccgcc
     1621 gcgacgcagc tcctcgaatt cagccgcgac ggcaagttca tcggcgagat cggccacaac
     1681 ctctatgcct ggtccttcgc gcacaccgtc aaggtcgacc ggcacgacaa catctgggtg
     1741 acggacaagg gctcggacat ggtgatcaag ttcacgcccg agggccgcgt cgccatggtg
     1801 ttcggccgga agcaggaggc atcggacgag gagaccggtc cgctgaagca tccgaacccg
     1861 ccgctcccgg ccgaggacgg acgcttccgc caggtgaccg acgtggcctt cgacgccgcc
     1921 gacaacacct atatcagcga cggctacatc aactcgcgcg tcgccaaggt ggacaaggac
     1981 ggcaactggc tcaaatcctg gggcgaccgt ggcacagggc cgggccagtt ccacacgccg
     2041 cacagcatcg ccacggacgc caagggcaac gtctatatcg ccgaccgcag caatcggcgc
     2101 atccaggtgt tcgacggcga aggcaacttc cagcgccaat tcaccatcga cctgcccgtg
     2161 ccggccgatg cgcggccggc gatcggcaac atgccgaacg aggcgatgat cgccggcggc
     2221 actttcgctc cgggctcgcc ctgggcgatc tgcatctcgc ccgggccgaa cccggtgctc
     2281 tacagctccg acgcgttccc gggccgcatc taccggctga gcctcgacgg caaggtgctc
     2341 ggcgtcctcg gccaagcggg caagcagctg aagcagttcg gctggatcca ccagatggcg
     2401 tgccccgcgc ccaacctgct ctacgtcgcc gagctcttga actggcgcgt gcagaagctg
     2461 gtgctccacg gctagcccag actcgcgcct ggaccgattc agacattgga gcccgcggcc
     2521 gacttgcgcg gtgcttctcc gcgcgcaata ccgtcgagca cataattcag gtgggatagg
     2581 gccgtctcga gcctctgcgt atctgccgcc cccaagctgt gatacagcgc cgacaactgc
     2641 cagcgcgccc gccagatgag ccgcgccgcg atttttcttc cgcgccccgt caaaagtacc
     2701 aggcaacagc gccgatcttt atccaccttg acgcgctcga cgagtcgctt gcgctcgagg
     2761 cagtcgaccg ccttcgaaag tgtcgcctgc ttgacgacag cgtattcagc aagctcgaac
     2821 atccgccgac cattgcgctt ggtcaaggcg acgagcacgc gctcctcggt tggagacaag
     2881 ccaaagtctt tcataggcgc gaccaggccc tcattgagca gctgggtcgc ctgcgctacc
     2941 agcgaggcca aagtcgtcat acgcgtcggc cgccgcatag cggctgacgg gcgacgggct
     3001 ttgtttggcg gtggagcgtg ccgggcgaga cagaaagcaa gctattgata acacggcaca
     3061 tcttagcatt ctcctgcact cattagactg cgtctaactc gcatcatgag ggaatgttac
     3121 attaaaatta ttccgagatg aacctctttc atcggccggg cccgcggtcg ggctaaatcc
     3181 cgagtgagtc gattcagccg gccgtttctc tgggcagcgt gaaataaaag gttgcgccgc
     3241 gtccggtgtt gtcggcggtc cacaaacggc cgccatggga ttcgatgatc gaccggctga
     3301 tcgcgagccc catgccggtg ccctgcggct tggtcgtgaa gaacgcgttg aacacctgct
     3361 ccgccttgcc cgcgggcagg cccacgccgg tatcgctgac cgagatcagc aattgaccat
     3421 cctcgacgac gtcggacttg atgatcagtt ctccgcccgt ctcgtgcatc gcctcgatgc
     3481 cgttgagcat gaggttcatg agcacctgct gcaattgcac gggatcggcc acgaccttcg
     3541 gaacctccgg ggcgaagttg ctgcgcatgg caatcgcatg tcgatcggcc tcctggcgca
     3601 gcagcgccat catttcaccg gcaatatcgt tcacatggac cagctcgcgt cgcggcgggc
     3661 cgcctttctt gtagaaggac cgcagccggt cgatgatctc ggccgcgcgg ctgccgtctt
     3721 tggcgattct catcgccgcc gcgcgcgcct cttccagatc aggctcggga cgcgtcaacc
     3781 atctgacgca ggcgttggcg ttggtgatcg cggcggcgat gggctgattg agctcgtgcg
     3841 ccagggaggc ggtgagcgcg ctcatcatgg tcatgcggtt catatgcgcg agctccgcct
     3901 cgagctggcg cagccgctcg cgctcttgtt cggcccgctt gcgttccgtg acgtcgaccg
     3961 tggtcccgac aaatgcggtg atctctccgc gagtgttgaa gaccggatgg ccggtcccct
     4021 cgacgtactt cacggcgccg tcgggcaaca ggactcggaa ttctccgatg tagtgagatt
     4081 tctcgcgaac cgccttttgc acacgcgcga aaaccgcctg ccgatcctcc gggtggatcc
     4141 gttccatcaa tgcatcgcgc gtcggcagcc cctgctgcgg atcgaagccg aaaattcgca
     4201 gcaactcctc cgagccgtac agcaattttc tcgtcgcggg atccgcggcc caacttcccg
     4261 tatgcgtcag cttctgcgct tccccgagat aagcttcgct gcgccgcagc ttctcctcgg
     4321 tgagcttgcg ctcgatggca attcccgcga ggtgggtgat ctgctcgatg atctcctgat
     4381 cgcgggggca cgggctgcgc ggctcgcgat aatacatcgc gaaggtgccg atcaccttgc
     4441 cttcggcgga catgatcggt gtcgaccagc aggcgcgcag cgaatgcggc aaagccaggt
     4501 cgcgataatc cgtccagagc gggtcgttcg cgatatcgga aacgacgacc tgtttgccgt
     4561 gccaggccgc cgctccgcaa gatccggcgg agggtccgat cgcggcgccc tcgatggcgt
     4621 cgacataggc cttgggaagg ctcggggccc cgccatgctt gacgcggccg tcttcgacca
     4681 gaaggatcga ggcgagcacg tcgggtgcgt gctcttctac gacgcggcac accgaatcga
     4741 ggatgcgcga cagcgtatcg cctctcgcga ccatctcgag gatgcgtctc tcgccggcca
     4801 gcagcgcgtc cgtccgcttg cgatcctcga cgtccgtcag ggtcccgtac caccggacga
     4861 tccggccctg ctcgtcgcgc agcggcaccg cccgcaccag gaaccagcgg tagtcgccct
     4921 tggcgctgcg atggcgcgcc tcgttctcga acggctcgcc gccggcgagc gagcggtgcc
     4981 acttggcgac gtggacatcg aggtcgtccg gatgaatcgt cgattgccag cccagtccct
     5041 tcgtctcctc cacggacaat cccgaatatt ccacccaacg ctgattgacc caggcgtcgc
     5101 ccggtgcgga gccggtcgag aacaccaagg ccggaatcgt atcgatcaca tcgcgaagca
     5161 ccttggcgcc gcggcgcacg gcctcttcgg cgcgcttctg ctcggtgatg tcttcgcata
     5221 ccaccaacag caccggccga ttctggagga cgacggcatt ggccttctcc cggacccaaa
     5281 gcatggtgcc gtccttgcga atcttcctcg cctcccacga catcttccgg ccgggttgcg
     5341 cgaagcaggc atcgacctgg gtccggacga aatcgcgatc gggctcatag aacacgctcg
     5401 agaccggctg cccgatcaat tcgctcacgc gataatcgag ctgctccgcg gccgcggcgt
     5461 tgatcgacaa gaacgcaccc gccgcatcga gaatgaagta catcgccgga ttgctctcga
     5521 aggcggctct ccattgctcc tcgatctgct cgcgggcatg ctcggcccgc ttgcggtcgg
     5581 tgacgtcctc gttgctcgcc aggatcgctg ccggcgcgcc gctctcgtcg cgctgcaacg
     5641 accaacggct tgccacgacg atctggctgc catccctccg cgtctgcacg agctcgcctt
     5701 cccaccggcc gctacgcatg acgtcgcgct cgatttgctc gagggacctc gggaaggccg
     5761 tcttcaacag cgcgtggacg acttcgccga tcgcctgctc cgccgtccat ccgtagagcg
     5821 cctcggcggc gcgattccag gagcggacga ctccgttcat atcgcacacg aaaatgccgt
     5881 catgcacgag gttcagcagg tcggcttgcg cccgcaaaat cgcttcggtc cgccacgcgt
     5941 cggtctgacg cgcgacgcga atggcttccg cgtccggcgc ggcggcagag cgaagaaacg
     6001 cgtcctcgtt atccccggtc atgacggatc gcctccaaaa ggctcgtcgg cagcctaccg
     6061 agcagcgccg ccgccgcaca aggtgcccgc agcatcgttc ggccgcgcct cagcgtccca
     6121 tttcggcgcg atgaggcttt cgcggcaggc ggcgtggcgc gatgcgttcc cggtagcgcg
     6181 ggattccgcc ggcacgctag actcaccgca taggccatcc tacgggaaga ccggagccct
     6241 tcgatgaaga agctcagtgt ggcggtgatc ggcgccggaa tggccggcct tgcgagcgcc
     6301 gcggccttgc agcgcgtcgg catcaacgtc accgtgtacg agcaggcgcg acgcttcgct
     6361 cgcctcggcg ccgggatcca gatcggctgc aatgccatgc aggttctgcg cgccttcggg
     6421 ctcgagccgg cgctgcgcgc agccgccttc tatccccgat cctggaacaa cagggactac
     6481 gataccggtg acacccgctt cgacatgatc ttcggcgagg ccgccgaaca gcgctacgga
     6541 actccctatc tgctcgcgca ccgcggcgat cttcacgccg cgctggcaag cgcggtaaac
     6601 gaggaggatc tgcgcctcgg ccaccagttg acgtcgctcg tatcgcgcgc ggagggcggc
     6661 atccgtctcg gcttcgcgaa cggtacggtc gcggaagtcg atgccgtcat cggcgccgac
     6721 ggcgtccact ccctggtcaa gacggttctg ttcggggagg accgtgcgaa catcactggt
     6781 cgcgtcgcct atcgcaccgt cttcccgagc gctttgctgc aagggtaccc cgtcgacgac
     6841 tgcacgaagt ggtgggggcc ggaccgccat atcgtcatct attacgtcaa gcccgaccgc
     6901 agcgagatct atttcgtcac tagccagccc gagccggagt tctcggtgga atcgtggtct
     6961 gccatgggca acgtcgacga gctgcgtgcc gccttcgacg gcttccatcc gcaggtgcgg
     7021 cacgtcctcg ccgcctgccc tgctgtgcac aagtgggcac tggtcgatcg cgatccgctg
     7081 ccgcgctggt gcgaaggcaa cgtcacgctg ctgggcgatg cctgccatcc gatgacgccc
     7141 tatatggcgc agggggccgc gatggcgatc gaggacgccg ccgtcctgtc gcgctgcctc
     7201 catggcgtcg atcgcgacgg catcgcggct gccttccgcc gcttcgaggc cagccgcaag
     7261 ccacgcacct cgcgcgtgca gttgagctcg cggaccaaca catggctgcg cgacccgacc
     7321 gacccgaact gggtctacgg ctacgacgcc ttgacagcgc cgctggccga gctggcgccg
     7381 tcgtcgaacg ccgcccgaag acaaccgacg ctatgagctc aaacccgagc ggtgtccccg
     7441 ttcatgcgcg accgtctctc atttgacgat ccatgaacgc gaggccgatt caggggaccg
     7501 cacagccttc aatggccggg atgtgacatg aaggcgcgcc taagcatctg ctgctcctcg
     7561 acgcaggcgt tgaaatcgcg gctgtcgggc tgaagcccgt tactggcgca ggtcgcctcg
     7621 tctcgggcgt ggagatcctc cacagacgcg cagccgctca ggctcgcgag catgagcaaa
     7681 actggaactg caagccgcat ctcgattccc ttcggacggc gcgccccgct cctcagatcg
     7741 acgccgcgcc gatcacggcg aaccgcgtcc gcagcagcag ggccacatct cctgaacgtt
     7801 gttacccgcc ggagccgtgg caagcatccg gcggcaaatt tcgatgggcg agccgaagcg
     7861 cccggaaata tttgccgtcg gcagatccgg accaggcaaa aaccgccact gcctcgagcg
     7921 ccgcgccgga aagctggata ttccgtctga cggaaaccgg cacagggctt gcagctgagc
     7981 agctgagaag atcgaaaagc gatctcgcgc caagaggcat accatgacag tctccggaat
     8041 caacaacctt ccaaatccgt ggcttttgca gcaggcccag caggcggcgg ctgccagcgc
     8101 agctgccgca gccgcggcga caccgcccgg aggacagacg acgcagggcg ctggggcgcc
     8161 ggcgtcgaac gccgcggtca accaattcag cgccatgatc caggcgatgg tgacgcagct
     8221 tcagtcggac gccagcagcg ccgggagcgc gacggcgccg gcagcggccg gagcggccgc
     8281 cggtcagacc tgcgccaatg cgaccaccgc cagcgcgacc gcttccggca cgacctccgc
     8341 cgcagcttcc ggccagacgg cgaccgccgc gaacacggca tccaacggtc agaacagtca
     8401 ggtcgatggc cattcccacg gccatcacca tcaccacggc ggcggagcgg acgaaacgca
     8461 gaagggcctg tcggcaacga accagacgac gctgcagacc gacgccaaca acctggtgga
     8521 cgcattgttc ggcgcgcagt cgagcgccaa cgccgccggt acaaccgcga cggctggaac
     8581 caccgcgacg accgcggcgg cgacgaccgc ggcggccacc tccgcggcga cgacgtcaac
     8641 cgccgcggcc gcgatgggcg ccagcacgct ggcagcctcg accacgaccg cgcagcctgg
     8701 cggaaccacg ccgcagacac tcgccgccgt cttcgcccag gacatgctca atgcgatcca
     8761 ggcatacacg gcgcagaatc cgaccggcat caccgcctcg gccctcaacg ccatcgcctg
     8821 agctcggcgc gaatacgcct cgaagggctg agccgatctc gcgcgccggc ggctacatgt
     8881 agcggcgggc acatcagatc atgcgcgggc agagagagag cgaaggacgt gacgaagccg
     8941 agccggaccg gacgcgacct tcccatcgtc attgtcggtc aggtcgacca cggcaagtcg
     9001 acgctcatcg gccgcttgct tcacgacacg ggctccctgc cggacggcaa ggtggcggcg
     9061 atcgaggaag cgagccggcg ccgcggcatg ccgttcgagt ggtccttcgt catggatgcg
     9121 ttcaaggcgg agcgcgacca aggcatcacc atcgacacca cccagatccg cttcagaagc
     9181 gaccgccgcc gctatatcat catcgacgcc cccggccatg ccgagtttct ccgaaacatg
     9241 ctgaccggcg cggcggcggc cgaggcggcg atcctggtgg tcgacgtcgt cgaaggcatg
     9301 tcggagcaga cgcggcgcca cgccttcctc ttgaagctgc tcggccttcg cgaggtcgtc
     9361 gtcgtcgtca acaagatgga ccgctgcggc tatgaccgca ccgcgttcaa tgggctcgcc
     9421 gcgatcgtca gcgcgtacct cgccgagctc gggctcgcgc cggcggcgat cattccgatc
     9481 tcggcgcgcg acggcgacgg catcgcccgg cgctcggcgc cgctcggctg gtacgcaggc
     9541 ccgaccatga tcgaggcgct cgacgcgctc gaaggcttcc ctagcgccgc cgatcggccg
     9601 ctgcgcttcc ccgtgcagga catctacaag ttcgacgatc gccgcatcat cgtcgggcgc
     9661 atcgacgccg gccgcctgcg cgtcggcgat cgcctctgct tctcgcccgg cgagcggacg
     9721 gcgcggatcg cctcgatcga ggggtggaac gcgccgccca cggtcaccgc ggcggccggc
     9781 cagtcgatcg ggctgacgct cgacgaggag atcttcgtcg agcgcgggca ggtggcgagc
     9841 gcagagggtg cagcgccgcc gatcgcgcgc cgactgaagc tgcggatctt ccatttcggc
     9901 agccagccgc tggcggtcga agatgaggtc aaggtgcaaa taggactggc ggagcgcgtt
     9961 gccaccgtcg aggccatcga gcgcgtcgtc gacgtcaacg acctgaccgg ccgccccgcg
    10021 gcggcggtga ggcgcaacga ggtcgccgat ctcgtggtgc tatgccggac accgctcgcc
    10081 gtcgacgaag gagccgatgc cggccatctc ggacgcggtg tcttgcgtcg cggccacgac
    10141 attgtcgcca gtgtccttgt cgaagcggcg ctcgatgcgc gccaacccgc cgcgtcccgc
    10201 cgccctatga cgccggtgcg ctcttcggtg agcgcgggtg agcgggcagc ggcctggggc
    10261 catcccggcg ccgtcctgtg gctgaccggc ttttcgggcg cggggaaatc gaccctcgcg
    10321 caggcgctcg agaaagaact cttccacgcc ggctggcgca cgatgctgct cgatgccgat
    10381 accttgcgcc acacgctcaa tgccgatctc ggattctccg aggccgagcg cgccgagaat
    10441 gtgcggcgga tcggcgcggt cgcgcagctc gtcgccgaaa gcggcgtggt cgccatcgtc
    10501 gcctgcatcg cgccgcggcg gatctatcgc gatcggctgc gcagcgagct cggcgctcgc
    10561 taccacgaga tccatgtgaa ggcggccctc gcggtgtgcg agcgccgtga cgtcgagagg
    10621 tcccatgcca aggcgaagcg cggcgcggtc gcggcgttca ccggcttgtc ggatccctac
    10681 gaagcgccgg ccgcacccga tctcgaagtg agcaccgacg tgctcacgcc gcgggagtgc
    10741 gtggatcagc ttctcgacta tgtcgaacgg acgctccggc cggccgacac ccgccagctc
    10801 gcgtcctgaa gcaccgccgg cagggagaag cgggaaatgg cagtgacgca gtaacgcgcc
    10861 agcggccagg cccgtcacat cggcggcttc acgccgtcct tctcggcgtt gacgcgcatc
    10921 gcgacgagac caccatcggc cttcttctga accagcacga gcaccgttgc tccgggcttc
    10981 agcaggttga catcgcccgg cagatacgcg acgaccggcg tcgtcgccgg caccatgatt
    11041 tcggacatct cgccctggtg gttgacggtg atggtgttgt cgctggtcgc gctcgagatg
    11101 ccggtgatgg cgccgttggt catcgtgctg ttggggacga ggtcccaggc gtactcgcct
    11161 tcaccagtgc cgcgcatggc ttcggggaag atgtggacct cgagcgcacg catcttcccg
    11221 tcgccgccgc ggaccgaggt gacgccgacg aagtcgccgc tcttgacgtc ggcgagggtc
    11281 cgcttggtca tggccacgac catcgtgccg ggcggaaagg cgattgacaa ctcttcacca
    11341 ctccgcgact tgaccgtgag cgtttcgccg tcaagcgccg cgacggtgcc gcggacgcgc
    11401 gtcggcggtc ctgccggagc ctgcgcgaga gccgaaaccg tgagcagggt gccgatcacg
    11461 gcaacaagat atctgagcga atgcaacgtc cgttctcccc tctctaaagg cgcctctacg
    11521 ctcctccgac gccatcccgg cgggtcgcgc gccgcgccgc gcgggcagga tatcgaatga
    11581 ggcgggagcc gacaacgtcg ctgtcatgct atggcaacat gctgcgacga tggttggcaa
    11641 cgcgacgatg ccgatctacg aagcgatcgt cattaaaaag cggcgctcac gcttccgtcg
    11701 tcgccgcgcc gcgcttcaag gcggcctcga tcgcttgcag gatgtcgtcg cggcggaagg
    11761 gcttgcggag gatcggcacg tcgccgacga cgtcgctcgc cggcgcgacg cccgaggtga
    11821 gcacgaccgg cgtgccgggg agacggcggc ggatctcgtg ggcgagctgg aagcccgaca
    11881 tgccgtcggg catgcgcacg tcgctgaaca ccacgtccac ggcaacgccg ctctcgagca
    11941 ggcgcaacgc cgccggaccg ctctccgcct cgatcgcgat gtagccgatg tcgtgcagca
    12001 gctcgatggc gaaggtgcgg acgtcctcgt cgtcctcgac caccagcact tttcgcgcgg
    12061 cgggcgcgct cgccgagact gccgccgccg cttgccgcca cgcctcggat gcgccgagcg
    12121 cgtcggtccg cggcaaatac aatcgcaccg tcgtgccggc cccgacggcg ctctcgatgg
    12181 tcacatggcc gcccgactgc cgcatgaagc cgtagacctg gctcaaaccg aggcccgtcc
    12241 ccttccccac ttccttggtc gtgaagaagg gatcgaaggc gcgctcgcgg atctcggcgg
    12301 gcatgccatg accggtatcc gtcaccgcga ccatgacata gcgcccggcc ggcggcagat
    12361 cgggcgtccg gtccggcatg tcggcgatct cagcctcggc ggtggcgacg gtgagagcgc
    12421 cgccgcgcgg catcgcatcc ttggcattga gggcgaggtt cagcatcgac gcctggagct
    12481 cggcgggatc gatgaggcag cagcccacgc cctcgccgag tgccagcagc actcggacct
    12541 cgtcgccgac cgcgcggcgg agcaggggct cggtgtcgcg cagcagcgcg ttgatgggga
    12601 tggcgcgcgg gcgcagcgcc cgctgccggg agaacgccag gagctggtcg gtcagcagaa
    12661 cgccgcgatg gatggcgcgc tgaatggtct cgacctgatc cctgagccgc acttccgtcg
    12721 cgcgcgcctg gatgagctcg agattgccgg cgatgaccgt cagcaggttg ttgaagtcgt
    12781 gcgcgacgcc ggcggtgagc tggcccacgg cctccatctt ttgcgcctgg cgcagctgcc
    12841 cttccgcccg ctcgcgctgc gccagcgcgg cgacgagctt ggcgttgctc tcggccagct
    12901 cgtgcgtgcg ctgccgctgc agcgtgaggt cggtgaccat gcccgagatg acgcgttgcc
    12961 cctgatcggg aaggtcgacg accgagagat agacggggat gccggtgccg tcgccggcgc
    13021 ggagctcgag ctcgccccgg cccccgccat gctcgctttc cgctacgagc cgggtgagga
    13081 cgccctggtc ggccgtcgcg acgaagtcgt gaaatcgccg gccgatgatc gcctcgccgg
    13141 gcaagcgaag gaggcggccc agcgatcggt tcgcgtaaag cacggcgccg tccggcgaga
    13201 gcgtcagcgc gccttcctgc atgcgctcga cgatgacgcg ataggggcgg tcggcgttct
    13261 cgagggcata gacctggggc ccggcctcgc cgttgacgac gacggcatcg acctggccct
    13321 cgcgcagcgc ctggagcgtc tcctcggctt cgcgcagccg cgcgcgcagc tcctcgatct
    13381 cgctgtcgcg atcgagcctc gctgcctgct tgtcaactgt cgcccgcatc gtcacgcccc
    13441 ggccgccgcc ttgctgccaa gccccagact gacgagaacg cgcgcgcggt cggaaagatc
    13501 gccgatgacg cgccggaggg gtgcggggcg cagcttgacg agggtgggcg ccgcaaagac
    13561 gttctcctcg gcggcggctt cgggatgctc gtagaggtcg acgacctcga gatcatggcg
    13621 gccgcggaga tgaccgtcgc agatctgtcg catattctcg atcgcacggg ccgaacgcgc
    13681 cgtgcttccc gtcacgtaga ggcgcagcac ataccgctcg tcattgccag cagatgggcc
    13741 gcgctcggtc aattatttct cctgagctcg atgtcgagcc ccacgagcac gcgctcggtc
    13801 tgcgagaggt cgccgatgat ccgccgcaac ggctccggca cgcggcgcac cagcgtcggt
    13861 atcgccagaa tctggtccat cgccgcgagc tgcggcttgt cgatgagatc gatgacctcg
    13921 acggcgtagc gcccggcgag atgcgtctcg cagatccgct tcagattggc gatcgccgcc
    13981 agcgatttcg gggtctgtcc cgcgacgtaa aggcgcaggt tgtagtgctc caggctcatc
    14041 gggtcctcat gtgctccgct gtgtccgacc ggcgccggct cttcggccgg cgggccgcat
    14101 cctcgccatt cggcttgacg ccacggctga cggcaatcgc cgaccggctc tcttcgcgca
    14161 gcgtctcctg cccctcctcc tgagcgatga gacgcgcgac ttctgcttcc tcggcctcga
    14221 tctcggcgcg cagctcggcg atctgccgct ccgccagcgc ccgcttgcgc tgcagctgag
    14281 cgcgctggcg ctcgaccgtc tcgcggcgcc gttgcgcctc ggcctggtcg cgcgcttctt
    14341 gcgcgacccg cgccgagccc gtcagcacgc cggccgcacc gagataaggc tcgatcattc
    14401 tcacgccctc acgcgtcaac gcatactcgc tgatctggtt cgagtggctc atgccgcgtg
    14461 acttcagcac gtagaggccg cgattgcgct cgccgttgct ctcgatgtcg atcagcgaga
    14521 tccaggtatc catgagcgag gacaggccga aatcgctctg cgcggcgcgc tcgtcggacg
    14581 tcgtgaggct ggtgaagacg gtggtgatgc cgcgcgactt cagcacatcc agcatgcgca
    14641 gcagcagggc gtgcacctcg gtctcggacc cgcggaatcc cgtcacgggg tcgacgatca
    14701 cgatggccgg ctggaacgca tcgagctcgc gatgcatgcg ggcgagatgc atctcgaagc
    14761 cataggcggt cggccgcgtc gtctcgaagc gcagccttcc ggcatcgaca tgaggctgga
    14821 gcatgatccc ggtcgaggcc atgttgcgga cgagctgtgc cgcggattcc tcgaagctga
    14881 agaagatgca gcgctcgccg cgcgcgcacg ccgcggcggc ggcggaagcg ccgagaatgg
    14941 tcttgccgga gccggcgacg ccggagatca gcacgctcga cccgcgatag acaccgccaa
    15001 cgccgagcat ggcatcgagg ccggcaacgc cggtcgacac cgcctcgcgg gagacagctc
    15061 ccacccgccc ggccgacgag agcggcacga cggtgatgcc ctggtcgtcg aggaggaagg
    15121 gatactcgtt catgccatgc gcggaaccgc gatatttgac gatgcgaagc cgccgcgtcg
    15181 tcacctgctc ctcgacgcga ttctcgagaa gaatgacgca gtccgagaca tattcctcga
    15241 ggccgtgccg cgtcagcgcg ccttcgccgc gctcgccggt gatgatggcg gtgaggccgc
    15301 gcaccttgag ccaggcgaag aggcgccgca gctcggcgcg cagaatggcg gtgttggaga
    15361 ggctcatgaa gagagtctcg agcgtgtcga gcacgacgcg cttggcgccg atcttgtcga
    15421 cggcgtgacc gaggcgcacg aacaagcctt cgagatcgta ttcgccgctc tcttcgatct
    15481 cgctgcgctc gatatggacg tgatcgatcg ccagtttctt cgccgcgatg aggcccggca
    15541 cgtcgaagcc gagcgacgcc acgttgtcgg cgagatcctt gcccgtctct tcgaagctga
    15601 cgaagacgcc gggctcgtcg aagcgcgtcg cgccattcac gaggaagatc atgctgagca
    15661 gcgtcttgcc gcagccaggg ccaccgcaga tcagcgtcgg gcgcccggcc ggcagaccgc
    15721 ccagcagaat gtcgtcgagg ccctcgatcc cggttggcgc cttgctcaat tgcgacagac
    15781 cgtgctcgat cttcttaggg cgctccgcct cccctccgcg catcgccatt acgcttgtcc
    15841 gcccgcatat ccgcccgcga ttttcttgat gcaagggtag ggtatatcga agcaaaggca
    15901 atggagaaac gctttatcgc cggctaatta ccctttcggg tgaacgcatt agacggaagt
    15961 cgcgggtcga tcgagacgtc atcgagcgat cagtggcagc ttcgaggccg cgctcaactt
    16021 ctcggcgagg cgctcaacgc ggcgcggaaa tcggcgcgcg gcggcaacca ccacgcgaga
    16081 tggactttcg tgcgccgcga cggtgacacg gcccacgatg cttgtcgatc tgacggtgcc
    16141 gccggcatcg cagcagaacc cgctatcctg ccggcgttgg cctggtcacg aaggagactg
    16201 cgagtcatgg cgcaacgacc ggcgcgccct cggacgccac gctcgacgcg gacgcaagcg
    16261 cgcggcgcga gcggccaagg cctggcatca ctccccgtct cgatcgagat cgagccgatg
    16321 gaagcgaagc tcgtggacgc gctgccggac ggcgcgggct ggcagttcga gccgaaatgg
    16381 gacgggttcc gctgtctcgc cgcccgggcc ggcgacgcga tcgatctccg cgccaaatcg
    16441 ggcaagccgc tcggacggta tttcccggac atggtcgcgc tgctgcgcgc gctcccggtc
    16501 gagcgcttcc tgctcgacgg cgaactcgcg atacaaatcg gcgacacgct cgccttcgat
    16561 gcgctgcagg cgcgcctcca tccggccgaa agccgcgtgc gcaaactcgc ggccgagacg
    16621 ccggccatcc tcatcctgtt cgatgtcctc gcaatcgacg gcgagagcct gctggcgtcg
    16681 acgctgtccg cgcgacgcag cgtgttggaa tcgttctacg ccgaggccgg agagcggaag
    16741 cggctcaagc tgtcgccctg gaccgacgac cgcgccgagg ctcagcgctg gctcgagcga
    16801 tcgggctgcg cgctggacgg cgtcatcgcc aagcggcgcg acgacatcta ccggccgggc
    16861 gagcgcgcga tgctcaaagt gaagcgccgg cgcagcgccg attgcgtcgt cggcggcttt
    16921 cgctacgcga gcggcggcgc agaggtcggc tcgctgcttc tcggcctcta cgacgacgcg
    16981 ggcaagctca accatgtcgg cttcacttcg acgatccatg acgtcgagcg gcacttgctg
    17041 acgcggcacc tcgagcaact gatcgagaag ccgggcttca ccggccgggc gcccggcggg
    17101 cccagccgat ggagcagcga gcgctcggcc gaatggcagc cgctgcgccc caggctcgtc
    17161 gtcgaggtgc gctacgacca ggtgaccgcc gaccgcttcc gccacggcac gaagctcctg
    17221 cgctggcgcc ccgacaaagc gccgcgccaa tgcaccgtcg atcagctcga gcgcgaagca
    17281 gcgccgagcg cactcatcga gcg
//
LOCUS       DASYNS010000067         6099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2045485, whole genome shotgun sequence.
ACCESSION   DASYNS010000067 DASYNS010000000
VERSION     DASYNS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2045485"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VGP50_02145"
     CDS             complement(<1..345)
                     /locus_tag="VGP50_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEV8026198.1"
                     /translation="MHSLDAFASEKLDRLERENLRRVIVPSVREEGIWVRRGGRRLLS
                     FSCNDYLNLTQHPRVKAAAIAAIEQYGTGAGASRLVTGDHPLYAVLEERLARLKGSDA
                     ACVFGSGYLANAG"
     gene            438..1703
                     /locus_tag="VGP50_02150"
     CDS             438..1703
                     /locus_tag="VGP50_02150"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014104569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEV8026199.1"
                     /translation="MTWLDDGLPHVWLPYAQMKTAAPPLAVARTDGTRIHLADGRVLV
                     DGVASWWTACHGYNHPHIRDAVRRQLELMPHVMFGGLANEPALTLARRLGAMLPPGLE
                     RVFFSDSGSVAVEVAVKMAVQYWLNRGIRGRTRLLAFSGAYHGDTFATMALCDPEDGM
                     HRLFAGLLPQHVIVDLPTDDATIAAFEAALARHGHELAAIIVEPLVQGAGGMLFHDAA
                     VLRRLRQAADRYDLLLIFDEIFTGFGRTGTLFAAAAAEVAPDIVALSKALTGGTMALA
                     ATVAQRKVFDAFWSDDPMHALMHGPTYMANALACAAANASLDLFATEPRLAQVAAIAA
                     SLERGLAPCRDLPGVRDVRVKGAIGVVELDRIDDLDGLKRRFVAAGVWLRPFGRIVYL
                     TPAFTIGDEDLRRLTAAVVDVLTERARLR"
     gene            1730..1969
                     /locus_tag="VGP50_02155"
     CDS             1730..1969
                     /locus_tag="VGP50_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEV8026200.1"
                     /translation="MAIVAYRVVQHDGGWAYKFDGVFSETFRTHAAAVAAAKRVAAEQ
                     RAPGERAEIQYEDANGVWRTEHVEGRDRPSTEVID"
     gene            complement(2083..3018)
                     /locus_tag="VGP50_02160"
     CDS             complement(2083..3018)
                     /locus_tag="VGP50_02160"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEV8026201.1"
                     /translation="MTETAKGSAWPALPLAAWRDTYATLHLWTQIVGKTRLALAPMEN
                     HWWQVPLYVTPRGLSTSAMPSGNRTLAVEFDFIDHVLSFAASDGATAVLPLVPRSVAE
                     FYAAFTATLRSLGFAAAIHPVPVEIEVAIPFADDREHASYDADAAHRCWQILVQSDRV
                     LKRFRGRFLGKASPVHFFWGSFDLAATRFSGRPAPRHPGGAPNCPDYVMVEAYSQECA
                     SCGFWPGGGAIAEPAFYAYAYPEPAGYGERPVRPDAAHYSAAAREFILPYDALRRAAD
                     PDDALLQFLQSTYEAAADLARWDRAALDRPRDAWP"
     gene            complement(3015..3293)
                     /locus_tag="VGP50_02165"
     CDS             complement(3015..3293)
                     /locus_tag="VGP50_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEV8026202.1"
                     /translation="MTAECTHLDQIHDVQPRTPEGCEECLKTGGTWVHLRLCLTCGHV
                     GCCDSSPGKHATKHFHRVRHPIVRSFQPGEDWAWCYIDEVMMEPAPVP"
     gene            3502..3954
                     /locus_tag="VGP50_02170"
     CDS             3502..3954
                     /locus_tag="VGP50_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026203.1"
                     /translation="MRKIGLFVALLLLAASPAWAAGKLDVVGKFSANGTEFDLAIYRE
                     NGETVALVGIAAGQRTSVAFSPAEWHSFVELWRQARAARSESWQPVGTFKETGTTELA
                     LLTVTAGPAVQFNIDGVKGHFTFVLPKSDFVAFDAKVAEMTAAAVATN"
     gene            complement(3982..5244)
                     /gene="glyA"
                     /locus_tag="VGP50_02175"
     CDS             complement(3982..5244)
                     /gene="glyA"
                     /locus_tag="VGP50_02175"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV8026204.1"
                     /translation="MADFRDMVRETDPEVADALDGEERRQQSGVEMIPSENYTYPEVL
                     ALLGSVFTNKYSEGYPGRRYYGGQEFTDKIEELARTRARALFRAGHANVQPLSGSPMN
                     QAVYLGFLKPGDTVLAMDLSHGGHLTHGAPVSHMGQLYHFVRYKTNPQQAGAIDFDAL
                     RTSAREVQPKMVLCGYSSYPRDLDYAAFKSIADEVGAITMADVSHIGGLIAAGVMRNP
                     LDAGFDFMTTTTHKSLRGPRGGMILCKTEHAKRVDSSVFPGLQGGPHMNTIAGVAVTL
                     KRAATPEFQAYGRDVLRNAKVLAESLMAQGMKLVTGGTDNHLMVVDMGASVGLDGAVA
                     ERVLDAAGITTNKQIIPDDPRPPLRPSGVRLGTPAATTRGMGEEEMRRLATLMVSALR
                     AADDPAALARLKAECAEMCRSFPVPGIA"
     regulatory      complement(5287..5375)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            5554..>6099
                     /locus_tag="VGP50_02180"
     CDS             5554..>6099
                     /locus_tag="VGP50_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8026205.1"
                     /translation="MSAQGSFIAAAALLALTATGAGAENAPGVTATEIKIGQTMPYSG
                     PASSYGVIGRSDTAFFRMINDKGGINGRKIVLISADDGYNPARAFEQTRKLVESDNVA
                     FIFDTLGTPSNSAIQKYLNERKIPQLFPSTGADKWGNYQDFPWTIGFQPSYRTEAEIY
                     AKYILKQRPNAKIAVLYQNDDF"
BASE COUNT         1010 a         2092 c         2034 g          963 t
ORIGIN      
        1 gccggcattg gcgaggtatc ccgagccgaa gacgcaggcg gcgtcgctgc ccttgagccg
       61 cgccagccgt tcctcgagca cggcatagag cggatgatcg ccggtgacga gccgcgacgc
      121 accggcgccg gtgccatatt gctcgatcgc cgcgatcgcc gccgccttga cgcgcggatg
      181 ctgcgtcagg ttgaggtagt cgttgcacga gaacgacaag agccgccgcc ccccgcgcct
      241 gacccagatc ccctcctccc gcacgctcgg cacgatcacg cgccggagat tctcccgctc
      301 caatcggtcg agtttctcgc tggcgaaagc gtcgagcgaa tgcatgcagc ttccggaacc
      361 aaatgcggtg cagtagagat ccctctcccc ccgccggaga ggggaaagct gcttagcccc
      421 tatggtgccg ccacacaatg acctggctcg acgacgggct gccgcatgtc tggctgccct
      481 atgcgcagat gaagacggcg gcgccgccgc tggcggtggc gcggacggac ggcacgcgca
      541 tccatctcgc cgatgggcgc gtgctcgtcg acggcgtcgc cagctggtgg acggcgtgcc
      601 atggctacaa ccatccgcat atccgcgacg ccgtgcggcg ccagctcgag ctcatgccgc
      661 atgtcatgtt cggcggcctc gccaacgagc cggcgctgac actggcgcgg cgcctcgggg
      721 cgatgctgcc gccggggctc gagcgcgtgt tcttctctga cagcggctcg gtcgcggtcg
      781 aggtcgcggt caagatggcc gtgcaatact ggctcaaccg cggcatccgc ggccggacgc
      841 gcctcctcgc cttcagcggc gcctatcacg gcgacacctt cgccaccatg gcgctctgcg
      901 atcccgagga cggcatgcac cgcctcttcg ccgggcttct gccgcagcat gtgatcgtcg
      961 acctgccgac cgacgatgcg acgatcgccg ccttcgaggc ggccctcgcg cgtcatggcc
     1021 atgagctcgc cgcgatcatc gtcgagccgc tggtgcaggg cgcgggcggc atgctgttcc
     1081 atgacgcggc cgtgctgcgg cgcctgcgcc aggctgccga ccgctacgat cttctgctca
     1141 tcttcgacga gatcttcacc ggcttcggcc gcaccggcac cctcttcgcc gccgccgcgg
     1201 cagaggtcgc gcccgacatc gtcgcgctgt cgaaggcgct caccggcggc accatggccc
     1261 tcgccgccac cgtggcgcag cgcaaggtct tcgacgcctt ctggtccgac gatcccatgc
     1321 atgcgctgat gcacgggccg acctacatgg cgaatgcgct cgcctgcgcc gccgccaacg
     1381 cctcgctcga cctcttcgcg accgagccgc gcttggcgca agtggccgcc atcgccgctt
     1441 cgctcgagcg cggcctggcg ccctgccgcg atcttccggg cgtgcgcgac gtccgtgtca
     1501 aaggcgcgat cggcgtcgtc gagctcgacc gcatcgacga tctcgacggg ctcaagcgcc
     1561 gcttcgtcgc ggccggcgtg tggctccggc cgttcggccg catcgtctat ctgacgcccg
     1621 ccttcacgat cggcgatgag gatctgcgcc gcctcaccgc cgccgtggtg gacgtgctga
     1681 cggaacgcgc gcgtctgcgc taagctcaag gcccaaggag gagaccggca tggcaatcgt
     1741 ggcgtatcgc gtcgttcagc acgacggcgg ctgggcctac aagtttgacg gcgtgttctc
     1801 ggagaccttc cgcacgcacg ccgcagcggt cgcggccgcc aagcgcgtcg ccgcggagca
     1861 gcgcgccccc ggcgagcggg ccgagatcca atacgaagac gcaaacggcg tctggcggac
     1921 cgagcacgtc gaaggccgcg accgcccctc gaccgaggtc atcgactgaa gcgggaccga
     1981 gtcaaacttc ccctctcccg catcgcggga gagggagggg cccagcgcga agcgctggga
     2041 gggtgagggt acgggtcagg catagtacat ttttcttccg cctcacggcc acgcgtcgcg
     2101 cgggcggtcg agcgccgcgc ggtcccagcg cgcgaggtcg gccgctgcct cgtaggtcga
     2161 ctgcaggaac tggagcaggg cgtcgtcggg atcggcggcg cggcggagcg cgtcgtaggg
     2221 caggatgaac tcgcgcgccg cggcgctgta atgcgcggca tcgggccgca ccggccgctc
     2281 gccatagccg gcgggctcgg gataggcata ggcgtagaaa gcgggctccg cgatcgcgcc
     2341 gccaccgggc cagaagccgc aactcgcgca ttcctgcgaa tacgcctcga ccatgacata
     2401 gtcggggcag ttgggcgcgc cgccgggatg gcgcggcgcc gggcgtcccg aaaagcgcgt
     2461 cgccgccaga tcgaagctgc cccagaagaa atgcacgggg ctcgccttgc cgaggaagcg
     2521 gccgcggaac cgcttcagca cacggtcgct ctggaccagg atctgccagc agcgatgcgc
     2581 ggcgtccgcg tcataggacg catgctcgcg gtcatcggcg aaagggatcg cgacctcgat
     2641 ctcgaccggc accgggtgga tcgcggcagc gaatccgagc gagcgcagcg tcgccgtaaa
     2701 agcggcgtag aactccgcga ccgagcgcgg gaccagcggc agcacagccg tggcgccgtc
     2761 gctcgccgcg aaggaaagga catggtcgat gaaatcgaac tcgaccgcga gcgtccggtt
     2821 gcccgacggc atggccgagg tggacaggcc gcgcggcgtc acatagagcg gcacctgcca
     2881 ccagtgattc tccatgggcg ccagcgccag gcgcgtcttg ccgacgatct gcgtccacag
     2941 atggagcgtc gcgtaagtgt cgcgccacgc ggcgagcggc agcgccggcc aggccgagcc
     3001 cttcgccgtc tcggtcatgg caccggcgcc ggttccatca tcacttcatc gatatagcac
     3061 caggcccaat cctcgccggg ctggaagctc cggacgatcg gatggcgcac gcggtggaaa
     3121 tgcttcgtcg catgcttgcc cggcgaagaa tcgcagcagc cgacatggcc gcaggtcaga
     3181 caaagccgaa gatgaaccca ggtcccgccg gtcttgaggc attcttcgca gccttccggc
     3241 gtgcggggct ggacatcgtg gatttgatcg agatgtgtgc attcggcggt catggtcttt
     3301 ctccatggcg tgctctttgg atactaacac cgctgccgcg ggggcgctcg cgggggcgac
     3361 gcgctcctca cggcagatat accgcattga ccgaaaagac gcctcgcatt gggcgagaag
     3421 gccggcgccg cttaaccgcc gtttcacgcc gtcctgccag gatcgatgcg cgtcctctcg
     3481 ccgccggaac tggagaagca tatgagaaag atcgggcttt tcgtcgctct actgcttttg
     3541 gcagcgtcgc ccgcctgggc cgcggggaag ctcgacgtgg tcggcaagtt ctcggccaac
     3601 gggactgagt tcgacctcgc catctatcgc gagaacggcg agaccgtggc gctggtcggc
     3661 atcgccgccg gacagcgcac ctcggtcgcc ttctcccccg ccgaatggca ttccttcgtc
     3721 gagctgtggc ggcaggcccg cgccgcgcgc agcgaaagct ggcagccggt cggcaccttc
     3781 aaggagaccg gcaccacgga gctggcgctg ctgacggtga ccgccggacc cgccgttcag
     3841 ttcaacatcg acggtgtcaa aggccatttc accttcgtct tgccgaagag cgatttcgtc
     3901 gccttcgacg cgaaggtcgc ggagatgacg gcggcggccg tcgcaacaaa ctgagcccgc
     3961 ggccacggcc ctagcccggg cttaggcgat ccccgggacc gggaacgacc ggcacatctc
     4021 cgcgcattcg gctttgaggc gcgccagcgc cgccgggtcg tcggcggcgc gcagcgcgga
     4081 aaccatcaat gtcgcgaggc gccgcatctc ttcctcgccc atgccgcgcg tcgtggctgc
     4141 gggcgtgccg aggcggacgc cgctcgggcg gagcggcggg cgcggatcgt cggggatgat
     4201 ctgcttgttg gtggtgatgc cggcggcgtc gaggacacgc tcggccacgg caccgtcgag
     4261 gccgacgctc gcccccatgt cgaccaccat cagatggttg tcggtgccgc cggtcaccag
     4321 cttcatgcct tgcgccatca ggctttcggc gaggaccttg gcattgcgca ggacgtcgcg
     4381 gccataggcc tggaactcgg gcgtggcggc gcgcttcagc gtgacggcga cgccggcgat
     4441 cgtgttcata tgcggcccgc cctgaagccc cggaaagacc gaggagtcga cccgcttcgc
     4501 atgctcggtc ttgcacagga tcatgccgcc gcgcgggccg cgcagcgatt tgtgcgtcgt
     4561 cgtcgtcatg aagtcgaagc cggcatcgag cggattgcgc atgacgccgg cggcgatgag
     4621 gccgccgata tggctgacat cggccatggt gatcgcgccg acctcgtccg cgatcgactt
     4681 gaacgcggca tagtcgaggt cacgcggata ggagctgtag ccgcacagca ccatcttcgg
     4741 ctgcacctcg cgcgcgctgg tgcgcagcgc gtcgaagtcg atggcgccgg cctgctgcgg
     4801 attggtcttg tagcggacga aatgatagag ctgacccata tgcgagacgg gcgcgccatg
     4861 cgtcagatgc ccgccatggg acagatccat cgccagcacc gtgtcgcccg gcttgaggaa
     4921 accgagatag acggcctggt tcatgggcga gcccgacagc ggctgcacat tggcatggcc
     4981 ggcgcggaag agggcgcggg cgcgcgtgcg cgccagctcc tcgatcttgt cggtgaattc
     5041 ctggccgcca tagtagcgcc ggcccggata gccttcggaa tacttgttgg tgaagaccga
     5101 gccgaggagc gccaggacct cgggataggt gtagttctcc gatgggatca tctcgacgcc
     5161 gctctgctgc cggcgctcct cgccgtcgag agcgtccgcc acctcgggat cggtctcacg
     5221 aaccatgtca cggaaatcag ccatcggtca ggggctcctt caaaacagaa acgtccgaga
     5281 ctccgaaggt cgtgcccagg cgaacggcgc gatgacgccc gcccgcgctc caccgtggtc
     5341 cattccacgt ctgctcgcca gtcgcgcggg ggtgagtgac cgtcagtccg gcaccaccat
     5401 acgcatgggg cgcgcggctc gcaagccgct ccggcccgga cgccattaag ccttcgctaa
     5461 tgggcggatg tgaaaatgca gagcccttcg tcatcccgcc aatgcgcgcg catgtaccac
     5521 atcggctgaa gcacgagctc ggagagtcca ttgatgtcag cacaagggtc tttcattgca
     5581 gcggccgcgc ttctcgctct gacggcgacc ggcgccggcg ccgagaatgc gccgggcgtc
     5641 accgccaccg agatcaagat cgggcagacg atgccctata gcggccccgc ctcgagctac
     5701 ggcgtcatcg gccggtccga tacggccttc ttccgcatga tcaacgacaa gggcggcatc
     5761 aacggccgca agatcgtgct catcagcgcg gatgacggct acaatccggc gcgagccttt
     5821 gagcagacgc gcaagctcgt cgagagcgac aatgtcgcct tcatcttcga tacgctcggc
     5881 acgccctcca actcggccat tcagaaatat ctgaacgagc ggaagatccc gcaactcttc
     5941 ccctccacgg gcgccgataa atggggcaac taccaggact tcccctggac catcggtttc
     6001 cagccgagct accgcaccga ggcggagatc tacgcgaaat acatcttgaa gcagcggccc
     6061 aatgccaaga tcgccgtgct ctatcagaat gacgatttc
//
LOCUS       DASYNS010000068         7809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1889095, whole genome shotgun sequence.
ACCESSION   DASYNS010000068 DASYNS010000000
VERSION     DASYNS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7809
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889095"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VGP50_02185"
     CDS             complement(<1..201)
                     /locus_tag="VGP50_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEV8026206.1"
                     /translation="METVWTPELIEQLSAFWKQGLSTAEIGRRLGVSKNAVVGKAHRL
                     SLDPRPSPLKRPPVRRVVGIAGP"
     gene            complement(533..1057)
                     /locus_tag="VGP50_02190"
     CDS             complement(533..1057)
                     /locus_tag="VGP50_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEV8026207.1"
                     /translation="MSTFPGGGEIFRLAGKVKWFNRSKGFGFIVPNDGGGDVFLPLSA
                     LERSGHTEAPDGASIVFDWTQGPKGRAVAQVIEMDTSTAPPRAPRENRSRGRDGEAEP
                     AGPAESLDGVVKWYDPARGFGFVLPNDGSKDIFVHITALRRSGIEMLEPGQAVRMMVA
                     QARRGREASSIELI"
     gene            complement(1172..1942)
                     /gene="ubiG"
                     /locus_tag="VGP50_02195"
     CDS             complement(1172..1942)
                     /gene="ubiG"
                     /locus_tag="VGP50_02195"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEV8026208.1"
                     /translation="MSKTTAEAAGGGTIDRAEVARFAKSAAAWWDPAGDFRPLHQINP
                     LRLAFIRDHLAVHFARDTRATRPFAGLRLLDIGCGGGLVSEPLARLGFAVTGIDAEAE
                     TVAVARRHAEAAGVAIDYRQAAAEDLAAAGQSFDVVLALEVVEHVADPALFLAAAAKL
                     VGPSGAFVAATINRTPKAFLFAILGAEYVLRWLPRGTHHWDKFLRPSELSEPLRRHGL
                     ALGALSGLAYNPFADRWALSADLDVNYLLFAARHAPAR"
     gene            2064..3284
                     /locus_tag="VGP50_02200"
     CDS             2064..3284
                     /locus_tag="VGP50_02200"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV8026209.1"
                     /translation="MARIVQKFGGTSVADIDRIKNVARRVKREVDAGNEVAVVVSAMA
                     GVTNQLVDWTRQTCRLHDAREYDVVVASGEQVTCGLVALALQDLGVNARSWLGWQIPI
                     RTDSMHGKARIAEIPVADMIRRMGEGQVAVVAGFQGIGADRRVTTLGRGGSDTSAVAL
                     AAALQAERCDIFTDVDGVYTTDPRIVAKARKLDRITYEEMLELASQGAKVLQTRSVEM
                     AMNHRVRVQVLSSFTDVPGTLVVDEDEIVEKQVVSGIAYSRDEAKITLLKVADRPGVA
                     AAIFGPLAENAINVDMIVQNISEDGRTTDLTFTVGKADLDRAVKVLEDRRTSLAYQSL
                     KPDANVVKISVIGVGMRSHAGVAQQMFKTLADKNINIQVISTSEIKVSVLIAEEYTEL
                     ALRALHTAYGLDAS"
     gene            3294..4430
                     /locus_tag="VGP50_02205"
     CDS             3294..4430
                     /locus_tag="VGP50_02205"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEV8026210.1"
                     /translation="MSPEADRRVTPGPEAAGRAAPDPAAERARRYLDRLWQRGRAFLG
                     CELAIMGGAMTWVSERHLVAAISNGGGFGVIACGAMTPALLDAEIAATRELTAQPFGV
                     NLITLHPALAELIEVCGRRQVGHVVLAGGLPSAPAIARIKGSGAKLLCFAPALSIAKK
                     LVRLGADAIVIEGMEAGGHIGPVATGVLAQEILPHIRDVPVFVAGGIGRGDAILAYLE
                     MGASGVQLGTRFVCAHESIAHPRFKQAFLRASARDAVPSVQVDPRFPVIPVRALGNEG
                     TRRFMEVQRAVVERFTRGEVAQKDAQLEIEHFWAGALRRAVVDGDVENGSLMAGQSVG
                     MVTREQPTREILDELVAQAVDALMARDRAREQHLREDERRQAGG"
     gene            4430..6700
                     /gene="ptsP"
                     /locus_tag="VGP50_02210"
     CDS             4430..6700
                     /gene="ptsP"
                     /locus_tag="VGP50_02210"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV8026211.1"
                     /translation="MESGAAVTGSRRLLRRLRDVMAGSGTAQERLDTIVRIIAADMVA
                     EVCSCYVMRAGEVLELFATEGLRREAVHRTRLRVGEGLVGNIAATARPLALANAQSHP
                     DFAYRPETGEEIYHSLMGVPILRGGRVLGVLVVQNKTQRHYTEDEIEALQTIAMVLAE
                     LAASGGLVSPLEQQPSEGIALLPMRLDGVRLNAGLASGVAVLHEPRVLIRQVVAEDPQ
                     NELARLSAAVAAMQTAIDDLLAASDVATSGEHRDILETYRMFAADRGWLQRMTEAVDS
                     GLTAEAAVQKVLEDTRSRLGQVSDPYLRERLADFEDLANRLQQHLAGRPVTAAAAELP
                     EEFILIARSMGPAELLDYDRRRLKGLVLEEGSPTSHVAVVARALDIPAVGRVKDVLAK
                     SEPGDLIVVDAEEAQVFVRPSEDIQQAVNARIEARLGKRLAYAALRDLPAQTRCGTRI
                     SVQLNAGLLLDLPYLDETGAEGIGLFRTELLFMLRDDFPSVGQQAATYRRVLEHAGER
                     PVTFRTLDIGGDKLLPYMSEIGDENPAMGWRAIRIALDRPALLRQQLRALIRAARGRR
                     LRVMFPMIAEVAEFDAARAVLDLEVARAAARNSPLPAKVEVGVMFEVPALLWELPQLL
                     PKLDFISVGTNDLVQFLFAADRGNPRLAERYDPLSLPVIRLLSEVVNSCAACNVPLAL
                     CGEMAGQPLDAMALLGIGFRVLSVAPGYVGPVKAMIRSLELAPLEGVLSGIAALPPRS
                     LRGKLRDFARDHGVVV"
     gene            6969..>7809
                     /locus_tag="VGP50_02215"
     CDS             6969..>7809
                     /locus_tag="VGP50_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8026212.1"
                     /translation="MEPESRPSRWHTIGQLLRETREGQGSDLNRVATALRIRAPYLEA
                     IEEGRYDSLPGAVYALGFVRTYAIHLGLDGEEAVRRFKLEAAGFDGQRDLTFPVPLGE
                     RSIPGATLVMAAVILVICGYGLFYYLTSSARPRPEHVPAVPSELALPRAPVATLPPSS
                     EAPAAEASSLALPAPSSSTAPATPSSPPGEPATASAPAVASLSPPSMAPARPSPAAPE
                     PALPAATVIAPPAPVQPAPAPPVPATATARPLAASEAAPAGAQAEKPRIFGLANGPTR
                     IVVH"
BASE COUNT         1212 a         2675 c         2663 g         1259 t
ORIGIN      
        1 cgggccggcg atgccgacga cgcggcgcac cggcggccgc ttcaggggcg acgggcgcgg
       61 atcgagcgac aggcgatgcg ccttgcccac cacggcattc ttgctcacgc ccaggcgccg
      121 accgatctcg gcggtcgaca gcccttgctt ccaaaaggcg gagagctgct cgatgagctc
      181 cggcgtccag acagtttcca ttgtatcccc gctgtattct gtgggtcccg cttgccatca
      241 ccacaatagg cagtatgtca ccggcggcga ccgaccgcaa atggattctt taaccatgcg
      301 cgacaatcgg gcgccgcggg tgcgactttc ctgtggaaat acgaccacga ccgtttgaga
      361 agaagcaggg catggcggca cggcctgcgc cgccgctttc gcgatcggca taccgctcga
      421 gcaggccttc ggcggattcg gtcagttcag gttctggaag ctctgaccct cgagcagatg
      481 ccgccggccc tggcgatcgg cgtgccgctc catgttttgt ccgcccattc gctcagatca
      541 gctcgatcga ggaggcttcg cggccgcgtc gcgcctgggc caccatcatg cgcacggcct
      601 ggcccggctc cagcatctcg atcccggaac gcctcagcgc agtgatatgc acgaagatat
      661 ccttgctgcc gtcattgggc agcacgaagc cgaagccacg cgccgggtcg taccatttga
      721 cgacgccatc gagcgactcg gcagggcccg ccggctccgc ctcgccgtcc cgcccgcgcg
      781 agcggttttc gcgcggcgcg cgcggcgggg ccgtcgatgt gtccatctcg atgacttgcg
      841 cgacggcacg gcctttcggg ccttgcgtcc agtcgaacac gatggatgcg ccatcgggcg
      901 cctcggtatg gccggagcgc tcaagcgccg agagcggcag aaacacgtcc ccgccgccgt
      961 cattcgggac gatgaagccg aaccccttgc tacgattgaa ccacttgacc ttgccggcga
     1021 gccggaagat ctcgccgccc cctgggaaag tactcatgac acacctgatt acgccccctc
     1081 cccctcaagg cacacgccgg cgccggccgg ggggcgtcct ccggccaagc cattacgact
     1141 cataaagtcg aatgccggct gcggcaagcg ttcatcgcgc gggagcgtgc cgcgcggcaa
     1201 agagcaggta gttgacgtcg aggtctgcgc tcaatgccca gcgatcggcg aaaggattgt
     1261 aggcaaggcc cgacaaggca ccgagcgcca gaccatggcg ccgcagcggc tccgacagct
     1321 cggatggccg gaggaacttg tcccagtgat gcgtgccgcg cggcagccac cggagcacat
     1381 attcggcgcc gaggatggcg aagagaaagg ccttgggcgt gcggttgatg gttgccgcga
     1441 caaaagcgcc gctgggtccc acgagcttcg ccgcggcggc gagaaagagc gcggggtcgg
     1501 cgacatgctc gacgacttcg agcgcaagca ccacgtcgaa gctctggccg gcggcggcga
     1561 gatcctcggc ggccgcttgg cggtaatcga tcgcgacgcc cgccgcctcg gcgtgacggc
     1621 gcgccacggc aaccgtttcg gcctcggcgt cgatgccggt cacggcgaag ccaaggcgcg
     1681 cgagcggctc gctcacgagt ccgccgccgc agccgatgtc gagcaggcga agcccggcaa
     1741 agggccgggt cgcacgggtg tcgcgggcga aatggacggc gagatggtcg cgaatgaagg
     1801 cgagacgcaa cgggttgatc tgatggagcg gccgaaagtc gcccgcgggg tcccaccagg
     1861 ctgctgcgct cttcgcgaac cgcgcaacct cggcgcgatc gatcgtgccg ccaccggcgg
     1921 cctcggccgt cgtcttgctc atctccgccg gtccccgcgc cttgcttcgc cgccgccggt
     1981 aagagtatgt atagccgccc ttcttccggc aatgggcggc ctccatcagc atcaattgtc
     2041 gcattcgagc ggttcctcat acaatggcgc gcattgtgca aaaattcggc ggcacgtcgg
     2101 tggccgacat cgatcgcatc aagaacgtcg cgcgccgcgt caagcgcgag gtcgatgccg
     2161 gcaacgaggt ggcggtggtc gtgtcggcga tggccggcgt caccaatcag ctcgtcgact
     2221 ggacgcgcca gacctgccgg ctgcatgacg cgcgcgaata cgatgtggtg gtcgcctcgg
     2281 gtgagcaggt gacctgcggc ctcgtcgcgc tggcgctgca ggatctgggc gtcaatgccc
     2341 gctcctggct cggctggcag atcccgatcc gcaccgacag catgcatggc aaggcgcgga
     2401 tcgcggagat tccggtcgcg gacatgatcc gccgcatggg cgagggccag gtggccgtcg
     2461 tcgccgggtt tcaaggaatc ggggcagacc gccgcgtgac gacactcggg cgcggcggct
     2521 cggacacctc ggcggtcgca ctcgccgcgg cgctccaggc cgagcgttgc gatattttta
     2581 cggatgtgga cggcgtctat accaccgatc cgcgcatcgt tgcgaaggcg cgcaagctcg
     2641 atagaattac ctacgaggaa atgctggagc ttgcctccca aggcgccaaa gtcctgcaga
     2701 cgcgctcggt cgaaatggca atgaaccacc gggtccgcgt ccaggtgttg tcgagcttta
     2761 ccgatgttcc gggaacgctg gtcgtcgacg aggacgagat cgtggaaaaa caagtggtga
     2821 gcggcatcgc ttatagccgc gacgaggcaa agatcacgct tctcaaagtg gccgaccggc
     2881 cgggcgtcgc ggcggcgatc ttcggcccgc tcgccgagaa tgcgatcaat gtcgacatga
     2941 tcgtccagaa catctcggag gacggccgca ccaccgacct cactttcacc gtcggcaagg
     3001 cggatctcga tcgcgccgtc aaagtgctcg aggaccggcg gacgagcctc gcctatcagt
     3061 cgctcaagcc cgacgccaac gtcgtcaaga tctccgtcat cggcgtcggc atgcgcagcc
     3121 atgccggcgt ggcgcagcag atgttcaaga cgctggcgga caagaacatc aacatccagg
     3181 tgatctcgac ctccgagatc aaggtgagcg tgctgatcgc cgaggagtat acggagctcg
     3241 ccctccgtgc gctccacacg gcttacggcc tcgatgccag ctgagattcg gccatgagcc
     3301 ccgaggcgga tcgccgcgtc actcccggcc ctgaggccgc aggccgcgcc gcgcccgacc
     3361 ccgccgccga gcgagcgcgg cgctatctcg accgcttgtg gcagcgcggc cgcgccttcc
     3421 tcggttgcga gctcgccatc atgggtggcg ccatgacctg ggtctccgag cgccacctcg
     3481 tcgccgccat ctcgaatggc ggcggtttcg gcgtcatcgc ttgcggcgcg atgacgcccg
     3541 cgctgctcga tgccgagatc gcggcgacgc gcgagctgac ggcgcagccc ttcggcgtga
     3601 acctgatcac gctgcatccg gcgctcgccg agctcatcga ggtctgcggc cggcgccagg
     3661 tcggccatgt ggtgctggcg ggcgggctgc cgagcgcgcc ggccatcgcg cggatcaagg
     3721 gctcgggcgc gaagcttctc tgctttgcgc cggcactatc catcgccaag aagctcgtgc
     3781 ggctcggcgc cgatgccatc gtcatcgaag ggatggaagc gggcgggcat atcggcccgg
     3841 tcgcgaccgg cgtgctggcg caggagattc tgccgcatat ccgcgacgtg ccggtcttcg
     3901 tcgccggcgg catcggccgc ggcgacgcga ttctggccta tctcgagatg ggagcgtcgg
     3961 gtgtgcagct cggcacgcga ttcgtctgcg cgcacgagtc catcgcgcat ccgcgcttca
     4021 agcaggcgtt cctgcgcgcg agcgcgcgcg acgcagtccc ctcggtccag gtcgacccgc
     4081 gtttcccggt gattcccgtg cgggcgctgg gcaatgaggg gacgcggcgc ttcatggagg
     4141 tgcagcgcgc ggtcgtcgag cgcttcaccc gcggcgaggt cgcgcagaag gacgcgcagc
     4201 tcgagatcga gcatttctgg gccggcgctt tgcgtcgcgc cgtcgtcgat ggcgatgtcg
     4261 agaacggctc gctcatggcc ggacaaagcg tcggcatggt gacgcgcgag cagccgaccc
     4321 gggagattct cgacgagctc gtcgctcaag cggtggacgc gctgatggcg cgcgaccgcg
     4381 ctcgggagca gcatctccgc gaggacgagc gccggcaggc gggcgggtaa tggaatccgg
     4441 cgcggcggtc accggatcgc gccgcctgct gcgacggctg cgggacgtga tggccggctc
     4501 cggcacggcg caggagcggc tcgacaccat cgtgcgcatc atcgccgccg acatggtggc
     4561 ggaggtgtgc tcctgttacg tcatgcgggc cggcgaagtg ctcgagctct tcgccaccga
     4621 aggcctgcgc cgcgaagccg tgcatcgcac gcgcctgcgc gtcggcgagg gcctcgtcgg
     4681 caacatcgcc gcaaccgctc ggccgctggc gctcgccaat gcccagtcgc atcccgattt
     4741 cgcctaccgc ccggagacgg gcgaggagat ctaccattcg ctgatgggtg tgccgatcct
     4801 gcgcggcggc cgcgtgctgg gcgtgctcgt cgtgcagaac aagacgcagc gccactacac
     4861 cgaggacgag atcgaggcgc tgcagaccat cgccatggtc ctggccgagc tggcggcgag
     4921 cggcggcctc gtcagcccgc tggagcagca gccctccgaa ggcattgcgc tcctgccgat
     4981 gcggctcgac ggggtgcgcc tcaatgccgg gctcgccagc ggcgtcgccg ttcttcacga
     5041 gccgcgcgtc ctcatccgcc aggtggtcgc cgaggatccg cagaacgagc tggcgcggct
     5101 cagcgccgcg gtggcggcga tgcagacggc gatcgacgac ctgctcgccg cgagcgatgt
     5161 cgcgacgagc ggcgagcatc gcgacattct cgagacctat cgcatgttcg ccgcggatcg
     5221 cggctggctg caacgcatga ccgaagcggt ggacagcggc ctcaccgccg aggcggcggt
     5281 acagaaagtg ctggaagaca cgcgcagccg cctcggccag gtgagcgatc cctatctgcg
     5341 cgagcggctt gccgacttcg aagacctcgc caaccggctg cagcagcatc tggccggacg
     5401 tcccgtcacg gccgcggcgg cggagctgcc cgaggaattc atcctcatcg cacggtcgat
     5461 ggggccggca gagctcctgg actacgaccg ccggcgcctc aaagggctgg tgctcgaaga
     5521 gggctcgccc acctcgcatg tggcggtggt cgcgcgtgcc ctcgacattc cggcggtcgg
     5581 ccgcgtcaag gacgtgctgg cgaagtcgga gccgggcgat ctcatcgtcg tcgatgccga
     5641 agaggcgcaa gtcttcgtgc ggccgagcga ggacatccag caggcggtga atgcgcggat
     5701 cgaggcgcgg ctcggcaagc gcctcgccta tgcggcgctc cgcgatcttc cggcgcagac
     5761 gcgctgcggc acgcgcatct cggtacaatt gaacgcgggg ctcctcctcg acctgcccta
     5821 tctcgacgag accggtgccg aaggaatcgg gctttttcgc accgagctcc tgttcatgct
     5881 gcgggatgat ttcccgtcgg tcggtcagca ggctgccaca taccgtcgcg ttttggagca
     5941 tgcgggcgag cgtccggtca cgttccgcac cctcgatatc ggcggcgaca agcttcttcc
     6001 ttacatgtcg gagatcggcg acgagaatcc ggccatgggg tggcgcgcca tccggatcgc
     6061 tctcgatcgc ccggcgctgc tgcgccagca gctccgcgca ttgatccgcg ccgcgcgcgg
     6121 gcgccggctg cgcgtgatgt tccccatgat cgccgaggtc gcggagttcg atgcggcgcg
     6181 ggcggtgctc gacctcgaag tcgcgcgggc ggcggcgcgc aactctccac tgccggccaa
     6241 ggtcgaggtc ggcgtcatgt tcgaggtgcc ggcgctcctc tgggagctgc cgcagctgtt
     6301 gcccaagctc gatttcatct cggtcggcac caatgacctg gtgcagttcc tgttcgccgc
     6361 cgatcgcggc aacccgcggc tggcggagcg ctacgacccg ctctccctgc cggtgatccg
     6421 cctgttgagc gaggtcgtca actcctgcgc ggcatgcaac gtgccattgg cgctctgcgg
     6481 cgagatggcg gggcagccgc tcgatgccat ggctctcctg ggcatcgggt tccgcgtcct
     6541 gtcggtggca cccggctatg tcggccctgt gaaggcgatg attcgcagcc ttgagctggc
     6601 gccgctcgaa ggcgtgctgt ccggcatcgc cgcactgccg ccgcgcagtc tcaggggcaa
     6661 gctgcgcgac ttcgcccggg atcacggcgt cgtcgtctga gaggggcgct gcggcagcgc
     6721 cgctcgcttt gacacaggcg gccgccgccc tcgagcgttg ccgcatccgc cccttgtgat
     6781 agagtggcgt cgtcgacggc atttagcgtc gcgattttac tgttttttta ctttcctctg
     6841 tcgcttatta cgcagagcga cagggcgaat cggtggccgg agtcatggca cttttttccc
     6901 gacgcaaaga ccctcttcac gaggcagctt tggaagccga gcagactgag gcggaacggc
     6961 caaccgtgat ggaaccagag tcgcgtccaa gccgctggca cacgatcggc cagctgctgc
     7021 gcgagactcg cgagggccag ggcagcgacc tcaaccgcgt cgcgacggcg ctgcgcattc
     7081 gcgcgcccta tctcgaggcc atcgaggaag gccgctacga cagcctgccg ggcgcggtct
     7141 acgcgctggg tttcgtgcgc acctatgcca ttcatctcgg cctcgacggc gaagaagcgg
     7201 tgcgccgctt caagctcgag gccgcaggct tcgacgggca gcgcgacctt accttcccgg
     7261 tgccgctcgg cgagcggagc atccccggcg ccacgctcgt catggccgcg gtcattctgg
     7321 tgatctgcgg ctacgggctc ttctactatc tgacttcgag cgcgcgcccg cggccggagc
     7381 atgtgccggc ggtcccatcg gagcttgcgc tgccgcgcgc gccggtcgcg acactgccgc
     7441 ccagcagcga agcaccggcg gccgaggctt catcgctggc attgcccgcg ccgtcgtcgt
     7501 caacggcgcc agcgactccg tcgtcacccc ccggcgagcc cgctactgcg tcggcgcctg
     7561 cggttgccag tctgagcccg ccatccatgg cgcccgcgcg cccgtcaccg gccgcgccgg
     7621 agccggcgtt accggcggca accgtgattg cgccaccggc gcccgtccag cccgcccccg
     7681 ctccgcctgt gcctgcgacg gcgaccgcgc gtcctcttgc cgcaagcgaa gccgctcccg
     7741 ccggcgcaca ggccgagaag ccgcggattt tcggactcgc caatggcccg acgcgcatcg
     7801 tcgtccatg
//
LOCUS       DASYNS010000069         6976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5477929, whole genome shotgun sequence.
ACCESSION   DASYNS010000069 DASYNS010000000
VERSION     DASYNS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5477929"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..2211
                     /locus_tag="VGP50_02220"
     CDS             340..2211
                     /locus_tag="VGP50_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026213.1"
                     /translation="MKTSLESLSLVDRVAVPALVVGANGRVIAANAVLAQVLGVEIAA
                     LRDSALADWLIDAALLEGFLRGAPGTCSEFVFRGGDGAARRLALTRALADAGGDLFTV
                     FDLTPLVAAEQQLREEMRRYQDMAGATSDFFFELDATMTRLRILRRARDGRLELLEQQ
                     RKWPDEVIDLTYDLDAFATVIRKMLAREPVRDYIHRQRDADGSERFLRSSGVPYYDAQ
                     GVFQGYRGVSVDVTAQVLAERAVRDSEARLRRNQQHLDQAQRVAATGSVERDLATGAE
                     EWSDEMYRLLNLERGTVAPSDEAVSALLHAEDRVRVQEGTRLARSGLAPRAGEFRIRR
                     ADGEIRTLYCETEFVRNDKGKAIRSMAVFRDVTELRAAELRQKDMERQLLHAQKLESL
                     GTLAGGIAHDLNNTLVPVLALTKITAERLPPESRERKNLVTVMRASERARDLVRQILA
                     FSRKEETERQPLDFAAVVREALGMLRASVPATVRITECIAEVPSVFGDAGKLHQVVIN
                     LVTNAAHAIGDAMGSISVELAVLEQEHAVRLTVVDTGCGMDETVMARIFEPFFTTKGV
                     GQGTGLGLSVVHGIVSGHGGRLEVKSRVGEGTSFAVYLPLAAEAAGPTEASAANAAD"
     gene            2337..2942
                     /locus_tag="VGP50_02225"
     CDS             2337..2942
                     /locus_tag="VGP50_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV8026214.1"
                     /translation="MKVVSWNLLRRTGAAVEDVARLIEREAPDLLLMQEATAEMEALP
                     DLAGGHFLREPLPGRIHGLAAWSPHPLPPPQTLPLPTSRMSVRSRPRLAQLLRIGAIT
                     FANVHLSHGQLLNRWQLLRIAGALEEGPAAVIGDYNAIGPTMLRGFRDVGPREPTHVA
                     QNVVPFRLDRCLVRGLHCAEARALERGPSDHRPIVLELRAA"
     gene            3067..3849
                     /gene="uppS"
                     /locus_tag="VGP50_02230"
     CDS             3067..3849
                     /gene="uppS"
                     /locus_tag="VGP50_02230"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEV8026215.1"
                     /translation="MPLILRLLYYLYERRLLAAIRRRQAPRHVGIILDGNRRHGRARG
                     MTEPEAMYRLGAGKLDDILDWCAELGIPAVTLWVFSTDNFRRSEAEVSGIFAAIAAKL
                     TALAEDPAIHRRRVKVRAIGRLDLVPPEVVAAITHAERATAAYDGIELNIAVAYGGRQ
                     EIVDAVRSWLGDMAASGATLAEAAAKVTPESIAEHLYTAGSPDPDLIIRTSGEIRLSG
                     FLLWQSAHSEFYFTDALWPALRKVDFLRAVRAFQERQRRFGQ"
     gene            complement(3986..5356)
                     /locus_tag="VGP50_02235"
     CDS             complement(3986..5356)
                     /locus_tag="VGP50_02235"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8026216.1"
                     /translation="MARHDLRKEPGRAAAATPLVAALREQMAALYRSGDRAGAMQVCR
                     QILAIAPKLADVLMIAGRIAAELGDDGEAVSFYETATAEKHDFAEAHYSLGNALTRLG
                     RIAAAAAAYRRAATHRPDLLPAHNNLGNALLSLERWDDAAEAYRGALALDEGIPDQHR
                     NLGIALQHGGHGDAALAAFRRALSLKPDWSRAYSNIANLLLERRAAAQVVETCDRWLA
                     ACPGTIEAMGLKSVALDQLGDRAGAQYLVDLERFVRVVQVDTAPPGFAGMDAFNRALT
                     EHALHHPTLTLPPEKDPRYHCPTLSITGEFLAEPKTAATAALERLFEAATRDYLAEMG
                     QENTHHPFAAKPPKRWRLTSWAAVLDRQGNLLPHIHYDGYVSGVYYCRIPAPIGSAEQ
                     GQAGWFELGRLPDHLGAGVAPLVRAIEPRAGMMILFPSYFYHRTIPFAAPGPRISIAF
                     DAMPTA"
     gene            complement(5482..6231)
                     /locus_tag="VGP50_02240"
     CDS             complement(5482..6231)
                     /locus_tag="VGP50_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV8026217.1"
                     /translation="MTTQTWNAAEYDKNARFVTDLGAPVLRLLAPQPGERILDIGCGD
                     GVLTKKIAAAGCDVVGLDSSPDFCAAARRLGLEVVEASAYDMTFAREFDAVFSNAALH
                     WMKDPDGLMAKVAAALRPNGRFVAECGGHRCVETVRRALIDELNQRGYDGAAADPWYF
                     PTPEDYHARLEAAGFAVRYIELIPRPTPLPADIVSWLAIFAQSFTKVLPEAERPEYLE
                     KVQTRTKPLLCDAAGNWTADYTRLRFEAYLE"
     gene            6450..6581
                     /locus_tag="VGP50_02245"
     CDS             6450..6581
                     /locus_tag="VGP50_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026218.1"
                     /translation="MIAVPVISRLFDMQGAIVILDQRPARIPQVIRDAGNDPSLYRF"
     gene            complement(6746..>6976)
                     /locus_tag="VGP50_02250"
     CDS             complement(6746..>6976)
                     /locus_tag="VGP50_02250"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV8026219.1"
                     /translation="ALLEREARAGALALDRPSFAAEQFLQMVVTLPQRRASGFGQPMT
                     EAELDAWADDCVNLFLNGCRFWRPATPPPGTR"
BASE COUNT         1117 a         2405 c         2329 g         1125 t
ORIGIN      
        1 gaccagccga aaagcaggtg gtggtcgatt gccgaccaca ggatgatcaa agcaaaggca
       61 agggtgatgg tcgcgccgac catcgtcacc ggcgagatgg cgaggagcct aagctctgcc
      121 gccttaacct tctccgcccg cactgcctcc acggaaccct ccttgggggt agagcctagg
      181 aagggaggct taatttttcc tgtgcggcgg ttcaccatgg ccatgcggct taagacggcg
      241 gcagctcggg gccggcgccc gaatgataaa aaactaacgg ttggtcaagg atagcgcagt
      301 accatcaaag gttgcgttct agggcctgcc ggagccgctt tgaagaccag tctcgagtcc
      361 ctgtcgttgg tcgaccgcgt cgccgtgccg gcgctggtgg tcggcgccaa cgggcgcgtc
      421 atcgcggcca atgccgtgtt ggcgcaggtg ctcggcgtcg agatcgccgc gctacgcgac
      481 agcgcgctcg ccgattggct catcgacgcg gcgctgctcg aggggtttct gcgcggcgct
      541 cccgggacgt gcagcgagtt cgtttttcgc ggcggcgacg gcgccgcgcg gcgtctcgcg
      601 ctgacgcgcg cgctcgcgga tgccggcggc gacctcttca ccgtcttcga cctgacgccg
      661 ctcgtcgccg ccgagcagca gctccgcgag gagatgcggc gctatcagga catggccggc
      721 gccacctccg actttttctt cgagctcgac gccaccatga cgcgactccg gatcctgcgc
      781 cgcgcccgcg acggccgcct cgagctcctg gagcagcagc gcaagtggcc ggacgaggtc
      841 atcgacctca cctacgacct cgacgccttc gccacggtca tccgcaagat gctggcgcgc
      901 gaaccggtgc gcgactacat ccaccgccag cgcgacgccg atggcagcga gcgcttcctg
      961 cgttcgagcg gcgtccctta ttacgatgcg caaggcgtct tccagggcta tcgcggcgtc
     1021 agcgtcgacg tgacggcgca ggtactggcc gagcgcgccg tgcgcgatag cgaggcgcgg
     1081 ctccggcgca accagcagca tctcgatcag gcgcagcgtg tcgccgctac cggcagcgtc
     1141 gagcgcgacc tcgccaccgg cgccgaggaa tggtccgacg agatgtaccg gctgctgaat
     1201 ctcgagcggg gcaccgtcgc cccgagcgac gaggccgtca gcgcgctgct tcacgcggag
     1261 gatcgcgtgc gggtgcagga aggtacccgc ctcgcccgga gcggcctcgc gccgcgcgcg
     1321 ggcgagttcc gtatccgccg cgccgacggc gagatccgca ctctttattg cgagaccgag
     1381 ttcgtccgca acgacaaagg caaggcgatc cgcagcatgg ccgtgttccg cgacgtgacc
     1441 gagctgcgcg ccgccgagct gcgccagaag gacatggagc gccagcttct tcatgcgcag
     1501 aagctcgaat ccctgggcac gctggcgggc ggtatcgccc atgatctcaa caacacgctc
     1561 gtgcccgtcc tcgccttgac caagatcacc gccgagcgcc tgccgcccga aagccgcgag
     1621 cgcaagaacc tagtcaccgt gatgcgggcg agcgagcgcg cccgcgatct ggtgcggcag
     1681 atcctggcct tcagccgcaa ggaggagacg gagcgtcagc cgctcgactt tgccgcggtg
     1741 gtgcgcgaag ccttgggcat gttgcgcgcc agcgtgccgg cgacagtgcg gatcaccgaa
     1801 tgcatcgccg aggtgccgag cgtcttcggc gacgccggca agcttcatca ggtcgtgatc
     1861 aatctcgtga ccaacgcggc ccatgcgatc ggcgacgcca tgggcagcat cagcgtcgag
     1921 ctcgcggtgc tcgagcagga gcacgccgtg cgcctcaccg tggtcgacac cggctgcggc
     1981 atggacgaaa cggtcatggc tcgcattttc gagcccttct tcaccacgaa aggcgtgggc
     2041 cagggaaccg gcctcggcct ctccgtcgtt catggcattg tctcgggtca tggcgggcgc
     2101 ctggaagtga agagccgcgt cggcgagggc acgagcttcg ccgtctatct gccgcttgcc
     2161 gccgaggcgg ccggtccgac cgaggcaagt gccgcgaatg ccgcggatta acgatgttgc
     2221 gccgcaaaaa cttgtgaagc cggtcgttat ccgttaatca cctgacgcta tgatccagcc
     2281 cgccttcgct gagcggtgaa ccggcgcggg actagacaac aagggatggc aagcacgtga
     2341 aggtcgtcag ctggaatctt ctgcgccgta ccggcgcagc cgtcgaggac gtcgcccgcc
     2401 tcatcgagcg cgaggcgccc gatctgctcc tcatgcagga ggcgaccgcc gagatggagg
     2461 ccttgccgga tctcgcgggc ggccacttcc tccgcgagcc tttgcccggc cgcattcacg
     2521 gcctcgccgc ctggagcccg catccgctgc cgccgcccca gacgctgccg ctgccgacct
     2581 cgcgtatgtc cgtgcgctcg cggccgcggc tcgcgcagct cctgcgcatc ggcgcgatca
     2641 ccttcgccaa tgtccatctc tcgcacggcc agctcttgaa ccgttggcag ctcttgcgca
     2701 tcgccggtgc cctcgaagaa ggcccggccg cggtgatcgg cgactacaac gccatcggtc
     2761 cgaccatgct ccggggcttc cgcgacgtcg gcccgcgcga gcccacccat gtggcgcaga
     2821 atgtcgtgcc gttccgcctc gaccgctgcc tggtgcgcgg cctccattgc gccgaagcac
     2881 gcgcgctgga gcgcgggccc tccgaccacc gtcccatcgt cctcgagctc cgcgccgcct
     2941 gatctcgcca agggtgagta gcggccgcgg gcgctcgccc gtgaccattg cggcttccac
     3001 gcgcgagctg gattcgcggt cgcgacaggg acccggctga tatggcatgg tgcggttgcc
     3061 gccccgatgc cgctgatcct ccgcctcctc tactacctct atgagcgccg cctcctggcg
     3121 gcgatccggc gccgccaggc gccgcgccat gtcggcatca tcctcgacgg caaccgccgc
     3181 cacggccgcg cccgcggcat gaccgagccc gaggcgatgt atcgcctggg cgccggcaag
     3241 ctcgacgaca tcctcgactg gtgcgccgag ctcggtattc ccgcggtcac gctctgggtg
     3301 ttctcgaccg acaatttccg ccgctccgag gccgaggtgt ccggcatctt cgccgccatt
     3361 gccgcgaagc tcacggcgct ggcggaggat ccggcgatcc atcgccgtcg cgtcaaggtg
     3421 cgcgccatcg gccgcctcga cctcgtgccg cccgaggtcg tcgcggcgat cacccatgcc
     3481 gagcgggcga ccgccgccta tgacgggatc gagctcaaca tcgccgttgc ttatggcggc
     3541 cgccaggaga tcgtcgacgc cgtccgctcc tggctcggcg acatggccgc gtcgggcgcg
     3601 acgctggccg aggccgccgc caaggtcacg cccgaatcca ttgccgagca tctctacacc
     3661 gcaggctcgc ccgatcccga cctcatcatc cgcactagcg gcgagatccg cctgtcgggc
     3721 ttcctccttt ggcagagcgc gcacagcgag ttctacttca ccgacgcgct ctggccggcc
     3781 ttgcgcaaag tcgacttcct ccgcgccgtc cgcgccttcc aggagcgcca gcgacgattc
     3841 ggccaataac gcgtgttgtc cctctcagcg cgaagcggga gggtgaggcg tcccctccgc
     3901 gagatgaccg agtccgccat cggcacccgc ctcacccgct cgctatgctc gcaccccccc
     3961 caagagcgga cggagagggt cgtgctcacg ccgtcggcat cgcgtcgaag gcgatggaga
     4021 tgcggggccc tggcgcggcg aaggggatgg tgcggtgata gaaataggag gggaagagga
     4081 tcatcatgcc cgcgcgcggt tcgatcgcgc gcacgagcgg tgcgacaccg gcgcccagat
     4141 ggtccggaag ccgcccgagc tcgaaccagc cggcctggcc ttgttctgcg ctgccgatgg
     4201 gcgccgggat ccggcagtaa tagacgccgc tgacatagcc gtcgtaatgg atatgcggca
     4261 gcagattgcc ctggcggtcg agcaccgccg cccaggaggt gaggcgccat cgcttcggcg
     4321 gcttcgcggc gaagggatga tgcgtattct cctgccccat ctcggcgaga tagtcgcgcg
     4381 tcgccgcctc gaacaagcgc tcgagtgccg cggtcgccgc cgtcttgggc tcggccagga
     4441 actcgccggt gatgctgaga gtcgggcagt gatagcgcgg gtccttctcg ggcggcagcg
     4501 tcagcgtcgg atgatggagc gcgtgctcgg tgagggcgcg gttgaaggcg tccatgccgg
     4561 cgaatcccgg cggcgccgtg tcgacctgga cgacgcgcac gaaacgctcg agatcgacca
     4621 ggtactgcgc gcccgcgcgg tcgccgagct ggtcgagcgc cacggatttg aggcccatcg
     4681 cctcgatcgt gccggggcag gccgcgagcc agcggtcgca ggtctcgacg acctgagcag
     4741 ccgcgcgccg ctcgagcagc agattggcga tattggaata ggcgcgcgac cagtccggct
     4801 tgagcgaaag cgcccggcgg aaagcggcga gcgccgcgtc gccatgtccg ccgtgctgca
     4861 gcgcgatgcc gagattacgg tgctgatcgg ggattccttc atcgagcgcc agcgcgccgc
     4921 gataggcctc ggccgcgtcg tcccagcgct cgagcgacaa gagcgcgttg ccgagattgt
     4981 tgtgcgccgg caggagatcg ggccgatgcg tcgcggcgcg gcgataggcc gcggccgcgg
     5041 ccgcgatccg gccgagccgc gtcagcgcat tgccgaggct gtaatgcgct tcggcgaagt
     5101 cgtgcttctc ggcggtcgct gtttcgtaga aggagacggc ttccccgtca tcgccgagct
     5161 ccgcggcgat gcgcccggcg atcatcagca catccgcgag cttgggcgcg atggcgagga
     5221 tctggcggca gacctgcatc gccccggcgc gatcgccgga ccggtagagc gccgccatct
     5281 gctcgcgtag cgcggcgacg agcggcgtcg ccgcggctgc tcgtcccggc tctttccgca
     5341 gatcgtggcg cgccatcgtg ttcgacaacc ccagagaacg cggccccgaa aggatagcac
     5401 ggcaccgatg tcgcgcaact cagggccgct gctttgtcct cgataattga agaactgaga
     5461 cggcggaaga gtggcggcgt ctcactccag atatgcctcg aagcgaagcc gcgtgtagtc
     5521 cgcagtccag tttccggcgg cgtcgcagag aagcggcttc gtccgggtct ggaccttctc
     5581 cagatactcc ggccgctctg cctccgggag caccttcgtg aagctctgcg cgaagatcgc
     5641 gagccagctc acgatgtccg ccggcaaggg cgtcggccgc gggatcagct cgatgtagcg
     5701 gacagcgaag cccgctgcct cgagccgcgc gtggtagtcc tcgggcgtcg ggaaatacca
     5761 gggatcggca gcggcgccgt catagccgcg ctggttcagc tcgtcgatga gcgcgcgccg
     5821 cacggtctcg acgcagcggt gcccgccgca ctcggcgacg aagcgcccgt tcggccgcaa
     5881 cgccgccgcg accttggcca tgagaccgtc cgggtccttc atccagtgaa gcgccgcatt
     5941 gctgaacacc gcgtcgaatt cccgcgcaaa ggtcatgtca taggcgctcg cttcgacgac
     6001 ctcgagcccg agccgccgcg cggcagcgca gaaatccgga ctggagtcga gcccgaccac
     6061 atcgcatccc gcagcggcga tcttcttcgt gagcacgccg tcgccgcagc cgatatcgag
     6121 aatgcgctct cccggctgcg gcgccagcag ccgcagcacc ggcgcgccca gatccgtgac
     6181 gaagcgcgca ttcttgtcgt attcggcagc gttccaggtt tgtgtggtca tcgcagttcc
     6241 gtccaaaggc gcattccgat caggccgagg cgatctcgcc ggtcgcgatg cgcatgacat
     6301 gcaccgcgtt gccgatgaca tcggccggcc gcttctcgcc ttggggccca tcaggcatcg
     6361 tcaaacatcc gatcgaccag tttctgaagc caccgcggcg gaggtccgct ctcacgtagg
     6421 aagtcgttta tgggcacgat gcgcttccga tgatcgccgt tccggtaatc agccgcttgt
     6481 tcgacatgca aggtgccatc gtcattttgg atcaacgtcc agcaagaatc ccgcaggtca
     6541 ttcgtgacgc tggcaatgat ccttcgcttt atcggttctg agcctatccg gctcttttat
     6601 gctcctgtcc aagcatgata tgggagcaac gcgccgatgg ccatagaccg tcaagccgga
     6661 acgtggatcg tcacaattca aactgaggca ccaccgcata gagacgatcg ctgccgtccg
     6721 tcggaaatcg tgcgcgctga cgggctcagc gcgtgccggg tggcggcgtg gccgggcgcc
     6781 agaagcggca gccattgagg aagagattga cgcaatcatc ggcccaggca tcgagctcgg
     6841 cctcggtcat gggctggccg aagcccgagg cgcggcgctg cggcagcgtg accaccatct
     6901 gcagaaattg ctcggcggcg aaggacggac gatcgagagc aagagcgccg gcgcgcgctt
     6961 cgcgctcgag gagcgc
//
LOCUS       DASYNS010000070         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4387177, whole genome shotgun sequence.
ACCESSION   DASYNS010000070 DASYNS010000000
VERSION     DASYNS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4387177"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1012
                     /locus_tag="VGP50_02255"
     CDS             143..1012
                     /locus_tag="VGP50_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026220.1"
                     /translation="MTETPKLAPLLRRVAEALERLAPPASPKNDLDAADAFVWHAERD
                     WLEPVPRVNRVDIDLLQGIDRVRDILLENTRRFAQGLPANNVLLWGARGMGKSSLVKA
                     AHAAVNAEAPRSLVLVEIHREDIPTLPRLLSLLRDTPRRCLLFCDDLSFDQQDTSYKS
                     LKAVLEGGIEGRPAHVVLYATSNRRHLLSRDMIENERSTALNPGEAVEEKVSLSDRFG
                     LWLGFHACDQETYHGMVRGYAARYGLDLAPEQLRAEASEWAITRGARSGRVAWQYIQD
                     LAGRLGKQIGGAD"
     gene            complement(1071..1463)
                     /locus_tag="VGP50_02260"
     CDS             complement(1071..1463)
                     /locus_tag="VGP50_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026221.1"
                     /translation="MTRRRIYLLAAGLLITIGLGAAGFHSLGLGQRTELPAEAMPYAE
                     EGPERAAFVANAMSSCAREVAAREPLGRPLSDDEIEAYCRCYSNGMAEVITADDVKQM
                     TAGAAPMDVVRDKAVRVLQSCSEVLPAK"
BASE COUNT          241 a          552 c          516 g          247 t
ORIGIN      
        1 ccctggcgca agatcccgtg gtggcccgga gctatctcgg cctttaacgc cgtgaccggc
       61 ggcgacattg acgctgcgcc gagcggtgct tagggtcgcg ccgccttcct gaccctcgag
      121 ccggagcgat cggcgcattt cgatgacgga gacccccaag ctggcacccc tgctgcggcg
      181 cgtcgcggag gcgctggagc gcctcgcgcc gcccgcgtcg ccgaagaacg acctcgacgc
      241 cgccgacgct ttcgtctggc acgccgaacg cgattggctc gagcccgtgc cgcgggtgaa
      301 ccgcgtcgac atcgatctcc tccagggcat cgaccgcgtg cgcgacatcc tgctcgagaa
      361 cacgcgccgc ttcgctcagg gattgccggc caacaacgtg ctgctgtggg gcgcgcgcgg
      421 catgggcaag agctcgctcg tcaaggcggc gcacgcggcg gtcaatgccg aggcgccgcg
      481 ctcgctcgtc ctggtcgaga tccaccgcga ggacatcccg acgctaccgc gcctgctgtc
      541 gctgttgcgc gacacgccgc ggcgctgcct ccttttctgt gacgacctct ccttcgatca
      601 gcaggacacc agctacaagt cgctcaaggc cgtgctcgaa ggcggcatcg aggggcggcc
      661 cgcccatgtc gtgctctacg cgacctcgaa ccggcgccat ctgctgtcgc gcgacatgat
      721 cgagaacgag cgctcgaccg cgctcaatcc gggcgaagcc gtcgaggaga aagtctcgct
      781 ctccgatcgc ttcggcctgt ggctgggctt ccacgcctgc gaccaggaga cctatcacgg
      841 catggtgcgc ggctatgccg cgcgctatgg gctggacctc gcgccggagc agctgcgcgc
      901 cgaggccagc gaatgggcaa tcacgcgcgg cgcccgctcg ggccgcgtcg cctggcaata
      961 catccaggac cttgccgggc gcctcggcaa gcagatcggc ggcgcggatt agttaggcga
     1021 ggatctctcg ctttcgcgcg agagagggcg caacgcgcgg acgacgccgc tcatttcgcc
     1081 ggcaagacct cgctgcagga ttggagaacg cgcaccgcct tgtcgcgcac gacgtccatc
     1141 ggcgctgccc ctgccgtcat ctgcttcacg tcatccgcgg tgatgacctc ggccatcccg
     1201 ttcgaataac agcggcagta agcctcgatc tcgtcgtccg agaggggccg tccaagaggc
     1261 tcccttgccg ccacctccct cgcgcaggat gacatggcat tggcgacgaa ggcggcccgc
     1321 tccggaccct cttcggcgta gggcatggcc tcggccggaa gctccgttct ttgcccgagc
     1381 ccgagggagt gaaaaccggc agcgccgagc ccgatcgtga tcagcaatcc ggcggccagg
     1441 agataaatcc ggcggcgcgt catcgcgcga tctcaggcgc tcggtcgatg acgggcgatg
     1501 ttgtcggtgc cgcgacgacg tctcacccgc tcaccgttgc gggcgtggcc gggaga
//
LOCUS       DASYNS010000071        11643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3138814, whole genome shotgun sequence.
ACCESSION   DASYNS010000071 DASYNS010000000
VERSION     DASYNS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3138814"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VGP50_02265"
     CDS             <1..229
                     /locus_tag="VGP50_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026222.1"
                     /translation="AIVPLSASALRKFPEIIAFPFFDSSGTSSLHAPERSGGKIRAKP
                     ELAAIPGASRKSAKRRSTSCIQAACFLANSA"
     gene            444..1046
                     /locus_tag="VGP50_02270"
     CDS             444..1046
                     /locus_tag="VGP50_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEV8026223.1"
                     /translation="MRTRHLALVVAAPVIFALSVIAAAPAGAHLVNAIGGGWSEGFAH
                     PFSGIDHLVAMVAVGIWATQLERRALSVLPLVFPVLMAIGAGLSFAGAPLPGVDDGIA
                     VSVAVLGVLLAVAARPRLWVSTTIVAIFGLFHGYAHGAEMPETAAPLLYGLGLLFGTA
                     VLLLIGIGLGMAARSPTGQRLLPVGAAAIAGVGITLILAL"
     gene            complement(1112..1681)
                     /locus_tag="VGP50_02275"
     CDS             complement(1112..1681)
                     /locus_tag="VGP50_02275"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV8026224.1"
                     /translation="MIGMQMRVICSLTVKQISTIPRYALSMRPLNGPDGASAPASAAD
                     RRHNTRRSAMWAAQLETARGERVSCIVLDVSAAGVRIQLEHSIAKDDIVLLITARVGA
                     HWCRVAWLDGVRVGLEFLESHTEAPTSSSSGVDAKFLRGRAGVLRRLALTATSLEAAG
                     KLIQSAREFEQAATALEQQQPTPSIRKGH"
     gene            1938..2507
                     /locus_tag="VGP50_02280"
     CDS             1938..2507
                     /locus_tag="VGP50_02280"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEV8026225.1"
                     /translation="MAQQAEGARRRAQEAGEAGRRATQDAAESAKRAAQDVTEGAKRV
                     AQDAAATTRRTAQDMAQRGAGTMLTVAEIYNETAQLTADELQAIATFSSIAAGGLAEM
                     RQAWMEWLNRSLRTSTRATQQLLHVKSIEEFADLQRSFLKESFDNLLEGSAQLLRISS
                     RVSGDACRPIEERVAHLRSQEPQQAEPRT"
     gene            2718..3995
                     /gene="oxlT"
                     /locus_tag="VGP50_02285"
     CDS             2718..3995
                     /gene="oxlT"
                     /locus_tag="VGP50_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEV8026226.1"
                     /translation="MSITEASPGQGRYRWLQLIAGIICMVMVANLQYGWNYFVDPINA
                     QHHWGRTDIQIAFTIFVATETWLVPIEGWFVDRFGPRIVVAFGGVTVAIAWSINSIAD
                     SLPALYGAQALAGIGAGAVYGTCVGNALKWFPDRRGLAAGLTAMGFGAGAAGTVYPIV
                     TMIKTYSYQSAFLWFGLGQGLICLILAQWLRAPKAGQAPKPAPKLGQTGRDYTPLEMA
                     RTPLFWLLYVMFVLVAAPGLVLTANVTPVAADYGISTTVVLLTFNVIVVAGFVDNILN
                     GVARPTFGWVSDHIGRENTMAIVFSCGALGYWALGTIGNTPWVFIVTAGLVYFTWGEI
                     YSLFPATCTDSFGPKYATTNAGLLYTAKGTASFLVPFASLFKSYAGSWQSVFVVAMAA
                     NLLVAACAFFVLKPMRARHNAGLLGRPQIRVAE"
     gene            4112..4462
                     /locus_tag="VGP50_02290"
     CDS             4112..4462
                     /locus_tag="VGP50_02290"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV8026227.1"
                     /translation="MPNYLLLLHQNAIRPRPSSPDAFLAVTKEYMAWTDKIRAEGRHK
                     AGDKLTDDAGKVIRADGGRTSVTDGPYAESKEVLGGYYIISAADYEDAVRVAQTCPHM
                     KFGGRIEVRQIEKM"
     gene            4467..5699
                     /locus_tag="VGP50_02295"
     CDS             4467..5699
                     /locus_tag="VGP50_02295"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEV8026228.1"
                     /translation="MAGADALVDDLFRREAGKISAWLARLLGPSRLELIEDAVQDAFG
                     AALARWPFSGVPEKPAAWLAIAARNKALDLLKREAKLGPLDESAAWRLGLSDPEETGR
                     LDDTLALMFVACHPALERDEQIMLTLKTVCGFGVAEIARAYLSTAEAVTQRLVRAKRK
                     IREIGLAFELPQGRELEERLPALIAAIYLLFNGGYTSGDGETLMTRELCAEALRLATL
                     LIEHPVTSIGECHALFALLSFHHARAEARTSDEGALILLAEQDRGRWDHALIARGFAH
                     LKKAMSSVALTTLHLEAAIAAAHAAAPSFAATDWHTIAHHYRALEELKPTPVVRLNAA
                     IAEAHAEGPARGLAQLDILAGERKLGRYALYHSARGTLLSQIGRSTEAAEAFQKALDC
                     PINDAERAFLTQKLHDCR"
     gene            5898..6224
                     /locus_tag="VGP50_02300"
     CDS             5898..6224
                     /locus_tag="VGP50_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026229.1"
                     /translation="MSRTSDITLFAAAALIAIGTGSALAQSANAAASESNGAVEIAPA
                     APETASRTGDIDADDTNAYRPDQVTGVNGSGNPGATGPGGAHDSRSGSGWNAGPRSKV
                     GGAHND"
     gene            complement(6403..8088)
                     /locus_tag="VGP50_02305"
     CDS             complement(6403..8088)
                     /locus_tag="VGP50_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEV8026230.1"
                     /translation="MAAAAYAQTAPNAAQLQALEAQVKELQRTTSQLQQVIEGLLASQ
                     KEEAAQVKAAQDQASQAQAQASQAQAQASQAQAQAAQTPGQAGELDSQGHAFLEHKKG
                     DALTFFTPGGEITGYGNFDVSIDAATKSLGNPTDQTGAHPVGNFGWMADISSNNSFLG
                     VRGFQRLPGMPLQFVYQFEAGIEIAATPGLKESNSNESNTVNGTLFNRNTYVGFYSPD
                     WGAIKIGKTDAPYKNATQALNPFAGELGDYAVVMGNTGGDNRVEFGTRLDHSIWYESP
                     TFGHVSFDVLFSPGQNRASDSDNLAAGESDCAGGNIPESGGDVPIACNDGAFSDVVSG
                     AIKYSNGPLYLTVAGELHHNVNRQSDITAIYGAGSVAALSGTQLAQFDQDVANEEAYK
                     AAALYTFPTKTLVGGIVEYLHRDVPEDLQFQNERQRWGTWLVLGQSLASADSVFLGWA
                     HAFHTPGDPGQHNDATLTTPDGVGTFASHNNQADLFTFAWKHFFTGNLSIYADVAATV
                     NGPSAHYDLGAGGRSVTTDCHDAFGATGGAASTPHCWTGQTLMGVSSGVKWTF"
     gene            complement(8423..11179)
                     /gene="fdhF"
                     /locus_tag="VGP50_02310"
     CDS             complement(8423..11179)
                     /gene="fdhF"
                     /locus_tag="VGP50_02310"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEV8026231.1"
                     /translation="MADAIKFTLDGDAVEAAPGETIWQVAQRQGVEIPHLCYSPAPGY
                     RPDGNCRACMVEIEGERVLAASCIRKPAPGMKVKTGSDRARFSRQMVFELLVGDQPAR
                     ATAHDPDSRFWRWADRVGVAGSRFPARAQPAPDRSHPAMAVNLDACIECTLCVRACRE
                     VQVNDVIGMKGRGHGEKIVFDFDDPMGESTCVACGECVQACPTGALMPATLVDEHNVF
                     TTKPDRQVDSVCPYCGVGCQLTYNIKDDKLLYVDGRDGPSNQNRLCVKGRFGFDYVHH
                     PHRLTHPLIRKDGVLKGDSHDWVDPANPWTHFRQASWEEALDRAAQGLKRIRDQHGGK
                     ALAGFGSAKGSNEEAYLFQKLVRTGFGTNNVDHCTRLCHASSVAALMEGIGSGAVTAP
                     FMAAKDADVIIVIGANPTENHPVAATFFKNAVKQGATLIVMDPRGQTLKRHATHMLQF
                     KPARDVALLSAMLNVIIGEGLYDKQYVQAHTEDFAKLAENVKNYPPEKMAEICGIDAE
                     TLRTVARKYARAEAAIIFWGMGISQHIHGTDNSRCLIALALVTGQVGRPGTGLHPLRG
                     QNNVQGASDAGLIPMYYPDYRPVQDDETRVFFEKLWGATLDPKKGLTVVEIVNAIHAD
                     QIRGMYIMGENPAMSDPDVEHAREALAKLEHLVVQDIFLTETAQYADVILPASAWPEK
                     IGTVSNTNRQVQMGRKALPLPGEAREDLSIIVDLAKRLGLPWTYTHPRDVFAEMKLAM
                     PSLDHITWERLERESSVTYPCDAEDKPGNDIVFGDGFPTLSGRGKLVPVGLVPPAEEP
                     DAQYPMVLTTGRQLEHWHTGAMTRRSSVLDDLEPEAVASLAPADLRRMGLKAGGKIRV
                     STRRGAIELMARADGAIPPGMVFIPFCYAEAAANVLTNPQLDPIGKIPEFKFCAAKVE
                     PAGA"
     gene            complement(11310..>11643)
                     /locus_tag="VGP50_02315"
     CDS             complement(11310..>11643)
                     /locus_tag="VGP50_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEV8026232.1"
                     /translation="LADLPLDFGTLEAQGCFIGSAAVVVLSDQDDMKAVALNLLRFFE
                     DESCGQCTPCRVGTEKAVKLMAAAPWDKPLLEELTRVMSDASICGLGQAAMNPVKMVM
                     KHFPEDVS"
BASE COUNT         1899 a         3816 c         3862 g         2066 t
ORIGIN      
        1 cgccattgtg ccgctcagtg ccagcgccct gcgcaaattc cccgagatca tcgccttccc
       61 cttctttgat agttcgggca cgagcagcct tcacgcgccc gagagatcgg gtggcaagat
      121 ccgggccaag cccgaattgg cagcgatacc aggagcttcg aggaagtcgg cgaaacgccg
      181 cagcacgtcc tgcatacagg ctgcgtgttt tttggccaat tccgcctaaa gaatacgcaa
      241 tcgcgttcat gaccactatt tcagcacact gcgcggatat tcagcgcagc gtcttgggga
      301 ggcgctggcg ccggaaccct aatagcctga tatcgctagc gttcttcgcg cgcggcgaag
      361 ttggtcccgc tcttgcttat ctcgtgtcta ttaatttggg ggcgagcgcg ctgatgcgcg
      421 cggcggcagg ggggaagagg accatgcgga ctcgtcattt ggcgttggtc gtggccgcgc
      481 cggtgatttt tgcgctctcg gtcatcgccg ccgcgccggc gggcgcgcat ctcgtcaatg
      541 cgatcggcgg cggctggagc gagggcttcg cccatccgtt ttccgggatc gatcatctgg
      601 tggcgatggt cgcggtcggc atctgggcga cgcaactcga gcgccgcgcg ctctcggtgc
      661 tgccgctggt ctttcccgtg ctcatggcca tcggcgccgg tttgagcttt gccggcgcgc
      721 cgctgccggg cgtcgacgac ggcattgccg tgtcggtcgc cgtcctgggc gtgctgcttg
      781 cggtcgcggc gcggccgcgg ctttgggtga gcacgaccat cgttgcaatc ttcggcctct
      841 tccatggcta cgcccatggc gccgagatgc cggagacggc ggcgcccctg ctctatggcc
      901 tgggcctgct cttcggcacc gccgtgctgc tgctgattgg catcggcctc ggcatggcgg
      961 cgcggagccc gacgggacag aggctgctgc cggtcggcgc cgcagccatt gccggcgtcg
     1021 gcatcacgct cattctcgcg ctttagtcca atatattccc atcgtcatgg cccgcggagc
     1081 catgcccgcc cagtaattgg tgcagtcggc gtcagtgccc tttgcggatc gagggcgtcg
     1141 gctgctgctg ctcgagcgcg gtcgcggcct gttcgaactc gcgggcggat tggatgagct
     1201 tgccggcggc ttcgaggctc gtcgccgtga gagccaggcg gcggaggacg cccgcgcgtc
     1261 cgcgcaggaa cttggcgtcg acacccgagg acgaggaggt cggcgcctcg gtgtggcttt
     1321 cgaggaattc gaggccgaca cgcacgccgt cgagccaggc gacgcggcac caatgcgcgc
     1381 caacgcgcgc cgtgatgagg agcacgatgt cgtccttggc gatggaatgc tcgagctgga
     1441 tcctgacccc ggctgccgag acatcgagca cgatgcagct cacccgctcg ccgcgcgccg
     1501 tctcgagctg cgcagcccac atggcacttc gccgcgtgtt gtggcggcgg tcggcagcgg
     1561 acgcgggcgc gctcgcgccg tcaggaccgt tgagcgggcg catggacaaa gcgtagcgcg
     1621 ggatggttga gatctgcttt acggtcaaac tgcaaataac ccgcatttgc attccgatca
     1681 tgcggcggca gaatccgaac cgaggaatta ctcagcgtcg gcgcgccgta acttgaccgt
     1741 gaaccgcgcc ccatagcggc gcagcgcgaa actctcgcgt cgcccatccg ttaatgtcgg
     1801 caacagccac agatccgtcg gggggagtcc tcgagacgcg tttggctcgc gagcaggcgg
     1861 gcatacgcac gcaaccggcg cttcgggtct ccgggcggac acggtcgcat catccaaccc
     1921 gagaaggagc aggagagatg gcccagcaag ctgaaggagc acgacgccga gcgcaagagg
     1981 cgggcgaagc gggccgccgc gcgacgcagg acgccgcgga aagcgcaaag cgcgcggccc
     2041 aggacgtgac ggaaggcgcc aagcgcgtgg cccaggacgc cgccgcgacg acgcggcgca
     2101 cggcgcagga catggcccag cgcggcgccg gcacgatgtt gacggtggcg gagatctata
     2161 acgagacggc gcagctcacg gccgacgaac ttcaggcgat cgctaccttc tctagcatcg
     2221 ccgcgggcgg cctcgccgaa atgcgccaag cctggatgga gtggctcaac cgcagcctgc
     2281 gcacctcgac gcgcgccacg caacagctgc tgcacgtcaa gtcgatcgag gaattcgccg
     2341 atctgcagcg cagcttcctc aaggagagct tcgacaatct cctcgaaggc agcgcgcagc
     2401 tgctgcgcat ctcgagccgc gtctccgggg atgcgtgcag gccgatcgag gagcgcgtcg
     2461 cccacctgcg ctcccaggag ccgcaacaag cagagccgcg cacctgacgg tctgatctga
     2521 tcatctgatc atcgcccgag ccaaacggct tccggccttc gagcggaagc cgccaagcga
     2581 tttttgccgg tcccgtgatc gcggcggctc gaccgcttgc cacccgcatg ctacggttcg
     2641 gcaaaagcgg ggcagctgcg ccgtcacgac accgcgccga ccgccccgtt ggtaagaaaa
     2701 caaacgctgg ggcggaaatg tcgatcaccg aagcatcgcc gggccagggc cgttatcgtt
     2761 ggctgcagct aatcgccggc atcatctgca tggtgatggt ggcgaacctc caatatggct
     2821 ggaattactt cgtcgatccg atcaacgctc aacatcattg gggccggacc gacatccaga
     2881 tcgccttcac catcttcgtc gccacggaga cctggctcgt cccgatcgaa gggtggttcg
     2941 tcgatcgctt cggaccgcgc atcgtcgtcg ccttcggcgg cgtcacggtc gccatcgcct
     3001 ggtcgatcaa ttcgatcgcc gattccttgc cggcgcttta tggggctcaa gcgctcgcgg
     3061 gcatcggcgc cggcgcggtc tatggcacct gtgtcggcaa cgcgctcaag tggttccccg
     3121 atcgccgcgg gcttgcggcg ggcctcaccg ccatgggctt cggcgcaggc gccgccggca
     3181 ccgtctatcc catcgtgacg atgatcaaga cctacagcta ccagagtgcg ttcttgtggt
     3241 tcggcctggg tcaaggcctt atctgcctca tcctcgcgca atggctgcgc gcgccgaaag
     3301 cgggacaggc accgaaaccg gcgcccaagc tcggccagac cgggcgcgac tacacgcctc
     3361 tcgagatggc gcggacgccc ctcttctggc tgctctacgt gatgttcgtg ctggtagcgg
     3421 cgccgggcct ggtattgacg gcaaatgtca cgcccgtggc cgcggactat ggcatcagca
     3481 cgaccgtcgt gctgctgacc ttcaacgtga tcgtggtcgc gggcttcgtc gacaacatct
     3541 tgaacggcgt cgcgcgcccg accttcgggt gggtatccga ccatatcggc cgcgagaaca
     3601 cgatggcgat cgtcttctct tgcggcgcgc tgggctactg ggcgctgggg accatcggca
     3661 acacgccctg ggtcttcatt gtgacggccg ggctcgtcta tttcacctgg ggcgagatct
     3721 atagcctctt ccccgcgacc tgcaccgaca gcttcgggcc gaaatacgcg acgaccaatg
     3781 cggggctgct ctatacggcc aagggcacgg cgtccttcct cgtcccgttt gccagtctgt
     3841 tcaagagcta cgccggtagc tggcaatcgg tgttcgtcgt cgccatggcc gccaatctcc
     3901 tggtcgcggc ttgcgccttc ttcgtgctga agccgatgcg cgcacggcac aacgccgggc
     3961 tcctgggccg accgcagatc cgcgtggcgg agtagggccg gccgtttcgc ggcacgtccc
     4021 gtcgaatgtc ggttcggcgc tctttcgatc gtcattagaa tgggcggacg gcgccgttgc
     4081 cgggatcgcc cgcgacaagg gagcctcatc catgccgaac tatctgttgt tgcttcacca
     4141 gaatgcgatc cggccgcgtc cgagttcgcc agacgcgttc ctcgccgtga ccaaggaata
     4201 catggcctgg accgacaaga tccgcgccga gggccgccac aaggccggtg acaagctcac
     4261 ggacgacgct ggcaaggtga tccgcgccga cggcggacgc acgtccgtga ccgacggccc
     4321 ttatgccgaa agcaaggaag tgctgggcgg ctattacatc atcagcgccg ccgactacga
     4381 ggacgcggtg cgcgtcgccc agacctgccc gcacatgaaa ttcggcgggc gcatcgaggt
     4441 gcgtcagatc gagaagatgt gagaaaatgg ccggggcgga cgcgctcgtc gacgacctct
     4501 tccggcgcga agccggcaag atctcggcct ggctcgcgcg tctcctcggc ccctcgcgcc
     4561 tcgagctgat cgaggacgcc gtgcaggacg cgttcggcgc ggcgctggcg cgctggcctt
     4621 tcagcggcgt gccggagaag ccggcggcgt ggctcgcgat cgccgcccgg aacaaggcgc
     4681 tcgatctcct gaagcgcgag gcgaagctgg ggccgctcga tgaaagcgcc gcttggcggc
     4741 tcgggctttc cgatcccgag gagaccgggc gcctcgacga cacgctcgcc ctcatgttcg
     4801 tcgcctgcca cccggcactc gagcgcgacg agcagatcat gctcaccctc aagaccgtgt
     4861 gcggcttcgg cgtcgctgag atcgcgcgcg catatctctc gacggcggaa gcggtgacgc
     4921 agcgcctggt gcgggccaag cgcaagatcc gcgagatcgg gctcgccttc gagttgccgc
     4981 aagggcgcga gctcgaggaa aggctgcccg cgctcatcgc cgcgatctat ctgctcttca
     5041 acggcggcta cacctcgggc gacggcgaga cgctgatgac gcgggagctc tgcgccgagg
     5101 cgctccgcct tgccacgctt ctgatcgagc accccgtgac ctccatcggc gaatgccatg
     5161 cgctcttcgc cctcctctcc ttccatcacg cgcgcgccga ggcgcgcacc agcgacgagg
     5221 gcgcgctgat cctgctggcg gagcaggatc gcgggcgctg ggaccacgcg ctcatcgcgc
     5281 gcggcttcgc gcatctgaag aaagcgatgt cctcggtggc gctcacgacg ctccatctcg
     5341 aggcggcgat cgccgccgcc catgccgcgg cgccctcctt cgcggcgacc gactggcaca
     5401 ccatcgcgca ccactatcgg gcgctcgaag agctgaagcc gacgccggtg gtgcgcctca
     5461 acgccgccat cgccgaagct catgcggagg gccccgcgcg cggactcgcg cagctcgaca
     5521 tcctcgccgg cgagcgcaag ctcggccgct atgccctcta tcattcggcg cgcggcacgc
     5581 tcttgtcgca gatcggacgg agcacggagg ccgccgaggc ttttcagaag gcgctcgact
     5641 gcccgatcaa cgacgccgag cgcgctttcc tcacgcaaaa actgcacgac tgccgctgag
     5701 cgactcgctc gctacagccg caagccgcgc aggcgtggat cctggcgacc ggtcggctcc
     5761 tgtagatttc ccgatgagcc cggaggcgag cttgtctcgc gaagtagcgc tttccgccaa
     5821 cgtatagtct agccgccggg actcatcgtc cccacgttaa tgacagggga acgtacgagc
     5881 agcctgtgga ggctcgattg tccaggacct cagatattac actcttcgcg gcagcagcgc
     5941 tgatcgcgat cgggaccggc agtgcccttg cccagagcgc caacgcagca gcgtcagaga
     6001 gcaacggcgc cgtcgaaatc gctccggcgg caccagagac ggcgtctcgc accggcgaca
     6061 tcgacgccga cgacaccaat gcctatcgtc ccgaccaggt gacgggcgtg aacggcagcg
     6121 gcaatcccgg cgcgacgggc ccgggagggg cgcatgacag ccgaagcggg agcggctgga
     6181 acgccggccc tcgcagcaag gtcggcggcg ctcacaacga ttgaccggag ccaggcctct
     6241 ttgggctatg gcttgccggc tcaacccaga ccgagggtga gctgatcgcg gtggcggccg
     6301 ccgagcgtca tggtttcgcg cgcggcgcgc gcgaccgaca caagcttctg gtgctgaaag
     6361 cgacggcttg ggccgtgcgt tctgacgcac ggcccaagcg gttcagaagg tccacttgac
     6421 gccgctggag acccccatca aggtttgccc cgtccaacaa tgcggggtcg atgccgcgcc
     6481 gcccgtcgcg ccgaaggcgt catggcaatc tgtcgttacc gaccggcctc ccgcgccgag
     6541 gtcgtaatgg gcggacgggc cgttgaccgt cgccgcgacg tcggcataga tgctcagatt
     6601 ccctgtgaaa aaatgcttcc aggcgaaggt gaacaggtcg gcctggttgt tgtgcgacgc
     6661 aaaggtgccg acgccgtcgg gcgtggtgag cgttgcgtcg ttgtgctggc ccgggtcgcc
     6721 cggcgtgtgg aacgcatgcg cccacccgag gaacacgctg tccgccgaag ccaggctctg
     6781 gccgaggacc agccaggtgc cccagcgctg gcgctcattc tggaactgca ggtcctcggg
     6841 cacgtcgcga tgcaaatatt cgacgatgcc gccgaccaag gtcttggtcg ggaatgtata
     6901 aagcgccgcc gccttgtagg cttcctcatt cgccacatcc tggtcgaact gggccaactg
     6961 cgtgcccgag agagcggcga cggagcccgc gccgtagatc gcggtgatgt cgctctgccg
     7021 attgacgttg tgatgaagct cgccggccac ggtgagatag agcggcccgt ttgaatactt
     7081 gatggcgccg ctcaccacat cgctgaaagc accgtcgttg caggcgatcg gcacgtcgcc
     7141 gccgctttcc gggatgttgc cgcccgcgca gtcggattcg cccgccgcaa gattgtcgct
     7201 gtcgctggcg cggttctggc ccggcgagaa cagcacatcg aagctgacat ggccgaaggt
     7261 cggcgattcg taccagatcg aatgatcgag cctggtgccg aattcgacgc ggttgtcgcc
     7321 ccccgtgttg cccatgacca ccgcgtagtc gcccaactcg ccggcaaacg ggttcagcgc
     7381 ctgcgtcgcg ttcttatacg gcgcatccgt cttgccgatc ttgatggcgc cccagtccgg
     7441 agaatagaac ccaacatagg tgttccggtt gaacaacgtg ccgttgacgg tgttgctctc
     7501 gttgctattg ctttctttga gacccggcgt cgcggcgatt tcgatgcccg cctcgaactg
     7561 atagacgaac tgcaacggca tgcccggcag tcgctggaag ccgcggacac ccaagaagga
     7621 attgttcgag gagatgtcgg ccatccaccc gaaatttccg accggatgag cgccggtctg
     7681 gtcagtggga ttgcccagac ttttcgtcgc cgcatcgatc gagacgtcga aattgccata
     7741 gccggtgatc tcgccgccgg gtgtaaagaa ggtgagcgcg tcgcccttct tatgctcgag
     7801 aaacgcgtga ccttggctgt cgagctcccc ggcttgtccg ggggtctgcg ccgcctgcgc
     7861 ttgcgcctgc gaggcctgtg cctgtgcttg gctcgcctgg gcctgcgctt ggctcgcttg
     7921 gtcttgcgcc gccttcacct gtgccgcctc ttccttttgg gacgccaaca gcccctcgat
     7981 cacctgctgc aactgactgg ttgtgcgctg cagttccttg acctgcgcct ccaacgcctg
     8041 aagctgtgcc gcattcggag ccgtttgtgc gtacgccgca gcggccatgc cgggtacgat
     8101 cagtccgatc gacgcaacgg tcgacaatag gcggcatgcg tgccgccgtc cccccttacg
     8161 catctatgtt tcccccatgg gctctatggc ttcgctggcg cagtcctcgc cctcggttgc
     8221 gcctttgatt tcgtatcaat ttattggtta acgagttgtt aacttcggca atttcgccgt
     8281 gcgcccgcgc ggaattcccg cgcttgtgtc gatcttgtct cgcaatgcaa gaacgcggtg
     8341 ttgccgacgc agcgcgcggc tgaaggcgcg tcgcggaaag atcggcgagg tgagcttttc
     8401 taccttgccg gagaaatccc tgttaagcgc ccgccggctc caccttcgcc gcgcagaact
     8461 tgaactccgg aatcttcccg atcgggtcga gctgcgggtt ggtgaggacg ttggcggcgg
     8521 cttcggcgta gcagaagggg atgaacacca tgccgggcgg gatggcaccg tcggcgcgcg
     8581 ccatcagctc gatggcgccg cgccgcgtcg agacgcggat cttgccgccg gccttgaggc
     8641 ccatgcggcg gagatcggca ggggcgaggc tcgccaccgc ttcgggctcg agatcgtcga
     8701 gcacggagga gcgccgcgtc atcgcgccgg tgtgccaatg ctcgagctgg cgtccggtgg
     8761 tcagcaccat cggatattgc gcgtcgggtt cttcggcggg cggcacgagg cccaccggca
     8821 cgagcttgcc gcggcccgaa agcgtcggga agccgtcgcc gaagacgatg tcgttgccag
     8881 gcttgtcctc ggcatcgcag gggtaggtga ccgagctctc gcgctcgagc cgctcccagg
     8941 tgatgtggtc gagcgacggc atggcgagct tcatctcggc gaagacgtcg cggggatgcg
     9001 tgtaggtcca gggcaggccc aggcgtttcg ccagatcgac gatgatcgac aaatcctcgc
     9061 gcgcctcgcc cggcagtggc agcgctttcc ggcccatctg cacctggcgg ttggtgttgg
     9121 agacggtgcc gatcttctcg ggccaggccg aggcggggag gatcacatcg gcatattgcg
     9181 cggtctcggt gaggaagatg tcctgcacga cgagatgctc gagcttggcg agcgcttcgc
     9241 gggcatgctc gacatcggga tcggacatcg ccgggttctc gcccatgatg tacatgccgc
     9301 ggatctggtc tgcatggatc gcattgacga tctcgacgac ggtcaagccc ttcttgggat
     9361 cgagcgtcgc gccccagagt ttctcgaaga agacgcgggt ctcgtcgtcc tgcaccgggc
     9421 ggtaatcggg gtaatacatc gggatcagac ccgcatccga cgcgccctgc acgttgttct
     9481 ggccgcgtag cggatgaagg ccggtgccgg gacggccgac ctgaccggtc acgagcgcca
     9541 gcgcgatcag gcagcgcgaa ttgtcggtgc cgtggatatg ctgcgagatg cccatgcccc
     9601 agaagatgat ggccgcctcg gcgcgggcgt atttgcgcgc gacggtccgc agcgtctcgg
     9661 cgtcgatgcc gcagatctcc gccatcttct ccggcgggta gttcttcacg ttctcagcga
     9721 gcttcgcgaa atcctcggta tgcgcctgga catactgctt gtcgtagagg ccttcgccga
     9781 tgatgacgtt gagcatggcc gagagcagcg ccacgtcgcg cgccggcttg aactgcagca
     9841 tatgcgtcgc gtggcgcttc agggtctggc cgcgcggatc catgacgatg agcgtggcgc
     9901 cctgcttcac cgcgttcttg aagaaggtcg cggcgaccgg gtggttctcg gtcgggttgg
     9961 cgccgatgac gatgatgacg tcggcatcct tggccgccat gaaaggcgcg gtcaccgcgc
    10021 cggagccgat gccctccatc agcgccgcca ccgacgaggc atggcagagc cgcgtgcagt
    10081 ggtcgacatt gttggtgccg aagccggtgc gtaccagctt ctggaagaga taggcctctt
    10141 cgttcgagcc cttggccgag ccaaagccgg cgagcgcctt gccgccatgc tggtcgcgga
    10201 tgcgcttcag gccttgcgcg gcgcggtcga gcgcttcctc ccagctcgcc tggcggaaat
    10261 gcgtccaggg attggcggga tcgacccaat catgcgaatc ccccttgagc acgccgtcct
    10321 tgcggatgag cgggtgggtg aggcgatggg gatgatggac gtaatcgaag ccgaagcggc
    10381 ccttgacgca gaggcggttc tggttcgacg ggccgtcgcg cccgtcgaca taaaggagct
    10441 tgtcgtcctt gatgttgtag gtgagctggc agccgacgcc gcaatagggg cagacgctgt
    10501 cgacctggcg atcgggcttg gtggtgaaga cgttgtgctc gtccaccagc gtcgccggca
    10561 tcagcgcgcc ggtgggacaa gcctggacgc attcgccgca ggcgacgcag gtgctctcgc
    10621 ccatgggatc gtcgaagtcg aagacgatct tctcgccatg gccgcgccct ttcatgccga
    10681 tcacgtcgtt cacctgcact tcgcggcagg cgcggacgca gagcgtgcat tcgatgcagg
    10741 cgtcgagatt gaccgccatg gcgggatggc tgcggtcggg cgcgggctgt gccctggcgg
    10801 ggaagcggct cccggcaacg ccgacgcgat cggcccagcg ccagaagcgg gagtccgggt
    10861 catgcgcggt ggcgcgcgcg ggctgatcgc cgaccagcag ctcgaacacc atctggcgcg
    10921 agaagcgcgc gcggtcggat ccggtcttca ccttcatgcc gggcgcgggc ttgcggatgc
    10981 aggacgccgc gagcacgcgc tcgccctcga tctcgaccat gcaggcgcgg caattgccgt
    11041 cggggcgata gcccggcgcc gggctgtagc agagatgcgg gatctcgacg ccctggcgct
    11101 gcgccacctg ccatatcgtc tcgcccggcg ccgcctcgac cgcgtcgccg tcgagcgtga
    11161 acttgatcgc atccgccatg gtcagtctcg ccaaagaaga gaagattgac cacagaggca
    11221 cagagacaca gagaagaaag atctgttggc gcgcaaagcg cgcccctcgc tcttctctgt
    11281 gtctctgtgc ctctgtggtg aaccttcttt cacgacacgt cctcggggaa atgcttcatc
    11341 accatcttga ccgggttcat cgccgcctgg cctaggccgc agatcgaagc atcgctcatc
    11401 acccgggtca actcttcgag gagcggcttg tcccaagggg cggccgccat cagcttcacg
    11461 gctttctcgg tgccgacgcg gcagggcgtg cattggccgc agctctcgtc ctcaaagaag
    11521 cgcaagaggt taagcgccac cgccttcatg tcgtcctggt ccgacagcac caccaccgcc
    11581 gccgagccga tgaagcagcc ctgggcctcg agcgtgccga aatcgagcgg caggtcggcg
    11641 agc
//
LOCUS       DASYNS010000072         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_487218, whole genome shotgun sequence.
ACCESSION   DASYNS010000072 DASYNS010000000
VERSION     DASYNS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487218"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VGP50_02320"
     CDS             complement(<1..572)
                     /locus_tag="VGP50_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8026233.1"
                     /translation="MAHDILVIDDEADIRMLMCGILRDEGYETREAGNSVQALVAIRS
                     RQPTLVILDIWLQGSELDGIEILKIIRRELPGLPVVMISGHGNIETAVAAIKIGAYEF
                     IEKPFKSDRLLLVAERAIEAARLKRENEELRLRAGGEQDLVGVSHAMTQLRQQVERVA
                     PTGSRVLVSGPAGAGKEVLARVLHAHSRRAR"
     gene            complement(562..2799)
                     /locus_tag="VGP50_02325"
     CDS             complement(562..2799)
                     /locus_tag="VGP50_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEV8026234.1"
                     /translation="MSVSRELVLWAGRVRLVRKTAITLAVAASASAIATYAAMRGRPL
                     FGSSRPEIDKLFLLLLILVLPLSAIVIWRLVQVWAERRRGLAGSRLHVRLVVLVSLVA
                     VIPTIFVAVPSYLLFKYGEQAWFSDPVRRALAESLQVAQRYLQEHQQTIRADIVSMAS
                     DLNRDAALLAVNPRRLDEIVGAQAELRSLSEAVVFDTDGRLLARSGFSLALQLEPVPD
                     WALQQANSGDVAIMTSENGRVRALVRLERFGNAYLYVGRFVDPIVLQHMEQTQGAVHE
                     YEERESQRRQWQITFSIAFFSLVLLLLTIAVLVGLRFATKMARPISSLIVAAEQVRSG
                     DLAARVPEGESDEEFGSLSRAFNRMTQQLQSQQSELIEANHQLDRRRRFTETVLSGVS
                     AGVIGLDQHGRIHLPNRSSSLLLGTDLDHLIGAELGEAVPEMRALFAEATRRPERLAQ
                     SQVQVARGGRSHTLLVRIAAERVEGEVKGYVVTFDDITELLSAQRKAAWADVARRIAH
                     EIKNPLTPILLSAERLKRKYLGEIKNDPETFTTCTETIIRHVGDIGRMVDEFSSFARM
                     PQPSIKDENLVDIARQIMFLHRTAAPEIRFDLDLPGAGAVRLACDARLVSQALVNLLK
                     NAAESIQSRGAAPPGVIALRLRESPQQTTVIVEDNGRGLPNEGRERLVEPYVTTRAKG
                     TGLGLAIVKKIMEDHGGELLLEDRDGGGARVLLVFNRGEARMPARAAVETGGPVVEKA
                     AAHGA"
BASE COUNT          480 a         1073 c         1049 g          464 t
ORIGIN      
        1 cgcgcgcgcc gcgagtgggc atgcagcacc cgcgccaaca cctccttgcc ggcgccggcc
       61 gggccgctca cgagcacgcg gctgccggtg ggcgccacgc gctcgacctg ctggcgcagc
      121 tgcgtcatcg catgcgagac gccgaccaga tcctgctcgc cgccggcgcg cagccgcagc
      181 tcctcgttct cgcgcttcag gcgcgccgcc tcgatcgccc gctcggcgac cagcaggagg
      241 cggtcggact tgaacggctt ctcgatgaac tcgtaggcgc cgatcttgat ggccgcgacc
      301 gccgtctcga tgttgccgtg gccgctgatc atgacgacgg gaaggccggg cagctcgcgg
      361 cggatgattt tcaggatctc gatgccgtcg agctcgctgc cttgcagcca gatatcgagg
      421 atcaccagcg tcggctggcg cgagcggatg gcgacgagcg cctggacgct gttgccggcc
      481 tcacgggtct catagccttc gtcccggagg atgccgcaca tgagcatccg gatatcggct
      541 tcgtcgtcga tgacgaggat gtcatgcgcc atgagccgcc gccttctcca ccactggacc
      601 acccgtctcc acggccgccc gggccggcat ccgcgcctcg ccgcgattga acaccagcag
      661 gacgcgcgcg ccgccgccgt cgcggtcctc gagcagcaac tcgccgccat ggtcttccat
      721 gatcttcttg acgatggcaa gcccaaggcc ggtgcccttg gcgcgcgtcg tcacataggg
      781 ctcgaccagc cgctcgcgtc cttcgttagg caggcctcgg ccgttgtcct cgacgatcac
      841 cgtcgtctgc tggggcgatt cgcgcaggcg cagcgcgatc acgccgggcg gcgccgcgcc
      901 gcgggactgg atcgactcgg cggcgttctt cagcagattg accagcgcct ggctcaccag
      961 ccgcgcgtcg caggcgagcc gcaccgcgcc cgcccccggc aaatcgagat cgaagcggat
     1021 ctcgggcgcc gccgtgcggt gcaggaacat gatctggcgc gcgatgtcga ccaggttctc
     1081 gtccttgatc gaaggttgcg gcatgcgcgc gaaggaagag aactcgtcga ccatgcggcc
     1141 gatgtcgccg acatggcgga tgatggtctc ggtgcaggtg gtgaaggtct cggggtcgtt
     1201 cttgatctcg cccagatatt tgcgcttgag ccgctcggcc gagagcagga tcggcgtcag
     1261 cgggttcttg atctcgtgcg cgatgcgccg cgccacgtcg gcccaggcgg ccttgcgctg
     1321 cgccgacagc agctcggtga tgtcgtcgaa ggtgacgaca tagcccttga cctcgccctc
     1381 gacgcgctcg gcggcgatgc ggacgagcag cgtatgcgag cggccgccgc gcgccacctg
     1441 gacctgggac tgcgccaacc gctccggccg gcgcgtcgcc tcggcgaaga gcgcccgcat
     1501 ctccggcacc gcctcgccca attccgcgcc gatcagatga tcgagatcgg tgccgaggag
     1561 cagcgaggac gagcggttcg gcagatggat gcggccatgc tgatcgaggc cgatgacgcc
     1621 cgccgagacg ccggagagca ccgtctccgt gaagcgccgg cgccggtcga gctggtggtt
     1681 ggcctcgatg agctcgctct gctggctctg gagctgctgc gtcatgcggt tgaaggcgcg
     1741 cgacagcgag ccgaactcct cgtcgctctc gccctcgggg acgcgcgcgg cgaggtcgcc
     1801 cgagcgcacc tgctcggcgg cgacgatgag cgagctgatg ggccgcgcca tcttggtggc
     1861 gaagcgcagg cccaccagca cggcgatggt gagcagcagc agcaccagcg agaagaacgc
     1921 gatcgagaag gtgatctgcc actgccggcg ctggctctcg cgctcctcgt attcgtggac
     1981 ggcgccctgc gtctgctcca tgtgctgcag cacgatcggg tcgacgaagc ggccgacata
     2041 gagataggcg ttgccgaagc gctcgaggcg caccagggcg cggacgcggc cgttctcgct
     2101 ggtcatgatg gcgacgtcgc cggaattggc ctgctgcagc gcccaatccg gcaccggctc
     2161 gagctggagc gccaggctga agccggagcg ggcgagcagc ctgccgtcgg tgtcgaacac
     2221 caccgcctcc gacagcgagc gcaattccgc ctgcgccccg acgatctcgt cgaggcggcg
     2281 cggattgacg gcgaggaggg ccgcgtcgcg gttcaggtcg ctcgccatgc tgacgatgtc
     2341 ggcgcggatc gtctgctgat gctcctggag gtagcgctgc gcgacttgga gcgactctgc
     2401 gagggcgcgc cgcaccggat cgctgaacca ggcctgctcg ccatatttga aaaggaggta
     2461 ggagggcacg gcgacgaaga tggtcgggat gaccgcgacc aggctcacca ggacgacgag
     2521 ccgcacatgg aggcgcgagc ccgcaagccc gcgccgccgt tccgcccaga cctgcaccag
     2581 ccgccagatg acgatggccg agagcggcag caccaggatc agcagcagca ggaacagctt
     2641 gtcgatctcc ggccggctcg agccgaagag cggccggccg cgcatcgccg cataggtggc
     2701 gatggccgag gctgacgccg cgaccgccag cgtgatcgct gtcttgcgga ccagcctgac
     2761 ccggccggcc cagaggacca gctctcgcga cacgctcatc ggcgcttcag cccccgtgat
     2821 ccccgcgcta cagcgtggca ttttaggcgc acagtcccgc cgctgtccaa caccagacgc
     2881 atttctatcg cgccggttgc ataacctccc tctcccctca gccggcggag tgacacggac
     2941 gcccgccatt tacccctctc ccgcaccgcg ggagagggag ggacccatcg cagcgatagg
     3001 aaggtgaggg ttgcagacgc ggcgataccc tcacccggct cgcagcgctc gccaccctct
     3061 cccgcg
//
LOCUS       DASYNS010000073         5451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4699785, whole genome shotgun sequence.
ACCESSION   DASYNS010000073 DASYNS010000000
VERSION     DASYNS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4699785"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VGP50_02330"
     CDS             <1..1479
                     /locus_tag="VGP50_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026235.1"
                     /translation="ATGILWGMLGLVTVSGTDMMNSVMVPLMIFSISASIASTHSCYV
                     PAVRAFIYPSYLFTALGEMIGGDAPHLVIAACALIYLANLELVARRGHRVVVQTLLSR
                     FAKERSQEQLARAQRVAAMGSAEYDIATGVEECSEEMYHLLGIAGPRSFPLTEENILA
                     AIHPQDRERVRAMRAVQRAGHRTRPGEFRVVRPDGEVRTLYAEIDIQRATAGRPLRVL
                     TIVKDVTALRAAEQRQKEMQQQLLQAQKLEAMGTLAGGVAHELNNTLVPVLGLAKLTI
                     KRLPEGSREQNNVITILQAGEKARDLVARIVAFSRKEMPTRVEVDLATLTRDTLSLMR
                     HSLPATIQLAERIEPVPPILGDPGQLPQIIINLVMNAVQATPERTGTVTVEVAPAQGE
                     KLLQMPERAPGVAVRLSVIDRGGGMDKATRARVFEPFFTTKTVGDGVGLGLSVVHGIV
                     TQHGGNIAVESSPGRGTRFDVYLPAAPITAVAPPATAETATA"
     gene            1835..2836
                     /locus_tag="VGP50_02335"
     CDS             1835..2836
                     /locus_tag="VGP50_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV8026236.1"
                     /translation="MTRTKVPLTVAALAAVVALAWHGPARAVEPGSSDPHLPGITDGL
                     PSGYVPPPGFYVTDDLAYISGSLQGGNGNAVKGPAATNITAYVDEPVLLWSTPLHLLG
                     AQYAVALVQPLTTVSITMGGVTTNKTGLTNTIIVPAILSWKLPVGFFVSGSFPIYLQD
                     GDTGDRGAGTSSQQHVANNTTTFAPSAAISWFNGQGLTASLNGEYDIQTQNDNFMNTA
                     FAHASYQSGDILNLDYTVQQQFGKWQFGVVGYYAVQTTDDQIKGLGAFGIPDGTVPAN
                     FAGLQNGSGNRFEKFAVGPLAQYDFGPATVNFRYTQDVYAKNTTQNGTFWFRVAFPL"
     regulatory      3035..3112
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            3454..4467
                     /locus_tag="VGP50_02340"
     CDS             3454..4467
                     /locus_tag="VGP50_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEV8026237.1"
                     /translation="MPMPGLAALRSAASVAAAPAETPASAHRLSRLPPLTALRAFVVA
                     ARHLSFARAAEELRVTPAAVGQQIRQLEAHLGCSLFDRNSRGLRLSEAGSALLPGLSE
                     GFERILEAVAQMSSRATARPLTVSVTPSFAAKWLVPRLEKFNAAFPGIDVRISASMAL
                     VDFAAEDVDCSIRYGSGAYPDLVVEKLLAESVFPVCAPALLAGAHPLDHPAALAHHVM
                     LHDDSPDRDASCPDWRMWLRAAGIPELNAEPGPRFNQSSLVLEAAIAGHGVALAKAQL
                     ALSDLRAGRLVRPFGAARRVEFAYYFVAPPERSDWPKVAAFREWLRREIREQPHDDSI
                     FTA"
     gene            4924..5112
                     /locus_tag="VGP50_02345"
     CDS             4924..5112
                     /locus_tag="VGP50_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026238.1"
                     /translation="MTKAMIVENREGAIGVAVEERGGFLFYAADAQYRAIDAKIFRRF
                     KDLERAAAVIDRSAPKRR"
     gene            5123..>5451
                     /locus_tag="VGP50_02350"
     CDS             5123..>5451
                     /locus_tag="VGP50_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026239.1"
                     /translation="MPVLDLVRIECLIAERERAALEPPRHRPRVEEAPRTRPLRRLAC
                     VVVAALVATVLLSPATAHATTLRIGVQKYGTLVLLQLRGTLTQRLAAAGVGVEWAEFP
                     SGPPLLEA"
BASE COUNT          891 a         1951 c         1721 g          888 t
ORIGIN      
        1 gccaccggca ttctctgggg catgctgggt ttggtgaccg tgtcgggcac cgacatgatg
       61 aacagcgtga tggtgccgct gatgatcttc tcgatctcgg cgagcatcgc ctcgacgcat
      121 agctgttatg tgccggccgt ccgggccttc atctatccgt cctacctgtt cacggctctg
      181 ggcgagatga tcggcggcga cgcgccccat ctcgtgatcg cggcgtgcgc gctcatctat
      241 ctcgccaacc tggagctggt ggcgcgtcgc ggtcatcgcg tcgtcgtcca gacgctgctg
      301 tcccggttcg cgaaggagcg cagccaggag cagttggcgc gcgcacagcg ggtcgcggcg
      361 atggggagcg ccgagtacga catcgccacc ggcgtcgaag aatgctcgga ggaaatgtat
      421 cacctgctgg gcatcgccgg gccgcgatcc tttcctctga cggaggagaa cattctcgcc
      481 gccatccatc cgcaggaccg cgagcgcgtg cgcgccatgc gcgccgtcca acgcgcgggg
      541 catcggacgc gccccgggga gttccgcgtc gtccgtcccg acggggaggt gaggacgctc
      601 tacgcggaga tcgacatcca gcgcgccacc gccggacggc cgctccgcgt gctgaccatc
      661 gtcaaggacg tgaccgcgct ccgcgcggcc gagcagcggc aaaaggagat gcagcagcag
      721 ctgcttcagg cgcagaagct cgaggcgatg ggcaccctgg ccggcggcgt cgcccatgag
      781 ctcaacaaca cgctcgtgcc ggtgctgggc ctcgccaagc tgacgatcaa gcgtctgccc
      841 gagggcagcc gcgagcagaa caacgtcatt accattcttc aggcgggcga gaaggcccgc
      901 gatctcgtcg cccgcatcgt cgccttctcg cgcaaggaga tgccgacccg tgtcgaggtc
      961 gatctcgcca ccctcacccg cgacacgctg agcttgatgc gccacagcct gcccgccacc
     1021 attcagctcg ccgagcggat cgagccggtg ccgccgatcc tcggcgatcc tggccagctg
     1081 ccgcagatca tcatcaacct ggtgatgaat gcggtccagg cgacaccgga gcggacgggt
     1141 acggtcaccg tcgaggtcgc tccggcgcag ggagagaagc tgctgcaaat gcccgagcgc
     1201 gcgccgggcg tggccgtgcg cctctccgtg atcgaccgcg gcggcggcat ggacaaggcg
     1261 acgcgggcgc gcgttttcga gcccttcttc accaccaaga ccgtcggcga cggcgtcggc
     1321 ctcggcctct ccgtcgtgca cggcatcgtc acgcagcacg gcggcaacat cgccgtcgag
     1381 agctcgccgg gccgcggcac gcgcttcgac gtctatctgc cggcggctcc catcacggcc
     1441 gtcgcgccgc ccgccaccgc agagactgcc accgcctgag tctcgcttcg gctcttcaag
     1501 atcgtcattc ctgcgaaagc agcaacccag ggccgcgcgc acgtcgcttg cccccgggtc
     1561 tccactcctc gtatgtcgcc gcacgcaaga gcgaggacgg aggaacgatt ctagggaacg
     1621 cgacgtgctg atttggcaaa atgatcatcg gccatcacgg gaagttgcgc gacaccggcg
     1681 aaacctcgct gtcgcccgcg cgaattttct cggcgaggcg cggatctagt cgacggagat
     1741 cgaagagaac gaagccgcgc agccgcgacc acggacggcg aagcgctgcg cgcttgccca
     1801 taggaggagc actgcaaaca tggagacgcc gacgatgacg agaaccaaag tacctttgac
     1861 cgtcgccgcg ctcgcggcgg tcgtggctct ggcgtggcat ggtcccgccc gagcggtcga
     1921 gccgggctcg agcgacccgc atttgcccgg catcaccgac ggccttccat cgggctacgt
     1981 gccgcccccg ggcttctatg tcaccgacga tctggcctat atctcgggga gtctgcaggg
     2041 cggcaacgga aacgccgtga aaggccccgc cgccacgaat atcaccgcct atgtcgacga
     2101 gcccgtgctg ttgtggtcga caccgcttca cctcctgggc gcccaatacg ccgtcgcgtt
     2161 ggttcagccg ctgacgaccg tcagcatcac catgggcggc gtcaccacga acaagaccgg
     2221 gctcaccaat accatcatcg ttcccgcgat cctgtcctgg aagctgccgg tcggattctt
     2281 cgtctcgggc agcttcccga tctacctgca agacggcgat accggcgatc gcggcgccgg
     2341 cacgtcgagc cagcagcatg tggccaacaa caccacgacc tttgcgcctt cggcggcgat
     2401 cagctggttc aacggccagg ggctgacggc ctcgctcaac ggcgaatacg acatccagac
     2461 ccagaacgac aacttcatga acaccgcctt cgcccatgcg agctatcaga gcggcgacat
     2521 cctcaatctc gactacaccg tccagcagca attcggcaag tggcagttcg gcgtcgtcgg
     2581 ctactacgcc gttcagacga ccgacgatca gataaagggc ctgggcgctt tcggcatccc
     2641 ggatggcact gtgcctgcga acttcgccgg gctccagaac ggcagcggca accgcttcga
     2701 gaaattcgcc gtcggccctc ttgcgcaata cgacttcggt ccggcgacgg tgaatttccg
     2761 ctacacccag gacgtctacg ccaagaacac cacccagaac ggcaccttct ggttccgcgt
     2821 cgccttcccg ctctaagcca cgcgcctcat tcccctctcc ccttgtggga gagggagggg
     2881 cccgttgcgc agcaacggga gggtgagggg tgcgcctcag cagccgcgct ctctggccgg
     2941 gcccagtgct ctgcccgaaa actgcgcatg ctcccgtata acacaacggc ttagtattta
     3001 ttttccattt gacattctcg cgaggttatt acgacaatgg caccgcgccg atttgagcgg
     3061 acagcttgcg ggcgcgtaac aaatgcagct aaagcgcctg gcacccgcca aggcttagag
     3121 ctgcggcggg cttctcgtcc cgagagcccg tttgcggaac cgatccgccc tcgccggcgg
     3181 atccacggag acgaggtgcg ccatgtcaca ctgcttcatt tctttggatg tccgcgcgac
     3241 gcgcagcgcc gcgcgacgcg ccagcgtcgt cgcgagcggg cgacagcggt tgctcttgca
     3301 accgcgagag ccccgcgccg gtgccgagcc cgccctcgag gaattgctcg ccgaccccgt
     3361 gctgcatctt gtcatgcgcc gcgacggcgt cagtcccggc gaattgcgcg ccgtcatcgc
     3421 cgcggcgcga gcacgaagac gggcgcgaaa ttcatgccta tgcccggcct agccgcgctc
     3481 cgatccgcgg cgagcgtcgc tgcggccccg gccgagacgc cggcgagcgc gcaccggctc
     3541 tcgcgcctcc cacccctgac cgcgctccgc gctttcgtcg tcgccgcccg gcatttgagc
     3601 tttgcgcgcg cggcggaaga gctgcgcgtc acgcccgccg ccgtcggcca gcagatccgc
     3661 cagctcgaag cgcatctcgg ctgcagcctc ttcgatcgca acagccgcgg cttgcgtctc
     3721 agcgaggccg ggagcgcatt gctgccgggc ctgagcgagg gcttcgagcg catcctcgag
     3781 gcggtggcgc agatgtcgtc gcgcgcgacg gcgcggccgc tgacggtctc cgtcacgccg
     3841 tccttcgccg cgaaatggct cgtgccgcgc ctcgagaaat tcaacgccgc attccccgga
     3901 atcgacgtgc gtatttcggc gtcgatggcg ctggtcgact tcgccgccga ggacgtcgat
     3961 tgctcgatcc gttatggcag cggcgcctat cccgacctcg tcgtcgagaa gctgctcgcc
     4021 gaaagcgttt ttcccgtctg cgccccggcg ctcctcgccg gcgcgcatcc cctcgatcat
     4081 cccgccgcgc tcgcgcatca cgtcatgctc cacgacgaca gccccgaccg cgacgcgagc
     4141 tgtcccgact ggcgcatgtg gctgcgcgcc gcagggatcc ctgagctcaa cgccgagccg
     4201 ggcccgcgct tcaatcaatc gagcctcgtc ctcgaggccg ccatcgccgg ccacggcgta
     4261 gcgctcgcca aggcgcagct cgcgctcagc gatctcaggg cggggcgcct cgtccgcccc
     4321 ttcggcgccg cccggcgcgt cgagttcgcc tactacttcg tcgcgccgcc ggagcgcagc
     4381 gactggccga aagtcgccgc cttccgtgaa tggctgcgcc gcgagatccg agagcagccg
     4441 cacgacgact cgatcttcac cgcgtgacgc cgcgcgacgc gcggcgtcat cgcgagcggg
     4501 cgccagcggt tgctcttgca accgcgaaag cccgcccgcc cgccggcgca ttcaaccgcc
     4561 acgcccacgc actcgagctt gataccacca gaaagttttt cgatgcgtgc cgcatagaag
     4621 aacttgtttg acccttgggg agcgctcgcg gatcatgatc ctcgggttcg atccaccgca
     4681 aacggatccg cggaatttga ggggcagctt gcgccgcgtc accaaccgct aaagcgccac
     4741 ggagcgatcc gtgggcccgt gtcttcaagg catggagaac cgctatgcgc tcgacttgtg
     4801 tttattcccg atcgacggtg tcgctgcttc ccggcgggcg ttgtcgccgc ccgacctagt
     4861 cttccgcaag cgcagcaaca caacagcagc atcggcctct gaatggccgt cgaggggagt
     4921 ctcatgacca aggcaatgat cgtcgaaaac cgggagggcg caatcggcgt cgccgtcgaa
     4981 gagcgcggcg gcttcctttt ctacgcggcc gacgcgcagt accgcgcgat cgacgccaag
     5041 atcttccgcc ggttcaaaga cctcgagcgc gccgccgcgg tcatcgaccg gtccgcgccg
     5101 aagcgtcgct gaggagtgcc tggtgcccgt cctcgatctc gttcggatcg aatgtctcat
     5161 cgccgagcgc gagcgcgccg cgctcgagcc gccgcggcat cggccccgcg ttgaagaagc
     5221 gccgcggacg agaccgcttc gccgcctcgc ctgcgtcgtg gtcgcggcgc ttgtcgcgac
     5281 cgtgctcctc tcgccggcga cggcgcacgc gacgacgctc cgcatcggcg tgcagaaata
     5341 cggaaccctc gtcctgctgc agctccgcgg cacgctcacc cagcggctcg ccgccgcggg
     5401 cgtcggcgtc gaatgggcgg agtttccctc gggcccgccg ctcttggagg c
//
LOCUS       DASYNS010000074         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5167625, whole genome shotgun sequence.
ACCESSION   DASYNS010000074 DASYNS010000000
VERSION     DASYNS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5167625"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /gene="shc"
                     /locus_tag="VGP50_02355"
     CDS             <1..1188
                     /gene="shc"
                     /locus_tag="VGP50_02355"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEV8026240.1"
                     /translation="KAEAFVSARLNGEDGLGGIYPAIANSVMMFECLGYAKDDPKLRV
                     AKAALRKLLVLAPGRSYCQPCFSPVWDTGLACHALMESGGDAADAAARRGLDWLAERQ
                     ILDVAGDWAEWRPTLRPGGWAFQYANPYYPDVDDTAVVAMALHRCDGARYRDAIERAA
                     EWIIGMQSRNGGWGAFDADNVYYYLNYIPFADHGALLDPPTADVSARCVGFLAQVDHS
                     CDPAVARGLDYLLGEQESDGSWFGRWGTNYIYGTWSALLALNAARVDKAEPAIRRAVA
                     WLVARQRPDGGWGEDGASYWAEAPRGEGKESTASQTAWALLALMAAGEVDHPSVAKGV
                     RYLLATQQAHGLWDEPWYTAVGFPRVFFLSYHGYPAFFPLWALARYRNLRRGSEEALG
                     YGM"
     gene            1188..1850
                     /locus_tag="VGP50_02360"
     CDS             1188..1850
                     /locus_tag="VGP50_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV8026241.1"
                     /translation="MAFAAVTGLAAEARIARRLGLRAVATGGRAERTADAAARLIAEG
                     ATGLLSFGICGGLDPALASGTLILPRAVQSETGERYLADAAAHRLCADALGGTLVTED
                     MLGAGSIVDTVARKAALFRRMAAVAIDNESHLVAAAATAAGIPFLVLRAVADPATRAL
                     PPAALIGLDDEGRPALLPVLRSLLVQPGQLGALLAIARDTSHALHALRRVAAIAPGIF
                     SR"
     gene            2148..>3227
                     /locus_tag="VGP50_02365"
     CDS             2148..>3227
                     /locus_tag="VGP50_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026242.1"
                     /translation="MLAWRVPVYIGGVIAIAALVDFALTPQSVYVFKGVEGPTEKTVI
                     PPPVAASWREYQAGGASRLAILLTDESSPWLGLAHGLKSIGVPFTITEDYAEAVRHRV
                     VMVYPMISGRLLSPAALKALAEVPANGGTLIGSYVLGGGLEDVFGIDKALPSQRHFEM
                     RFDRPAAERFGLRDPRELVISIGNQDKTEVFGTYGYVGVTGTALASFEDGQAAITEHR
                     LGSGRAYALGIDLGELLMQAYNNREEAFSRAYVNEFEPSIDVLLRVIRDIYRSGEPQA
                     ATLGTVPFNRPLAVMITHDIDYTRSLNNAVDYATYEKSQGVRATYFMQTKYVRDYNDE
                     IMLNDDTAPLLRRLGTLGMEVGSHTV"
BASE COUNT          460 a         1170 c         1054 g          543 t
ORIGIN      
        1 aaggccgagg ccttcgtcag cgcgcggctc aacggcgagg acgggctcgg cggcatctac
       61 ccggcgatcg ccaactccgt gatgatgttc gagtgcctgg gctatgccaa ggacgacccg
      121 aagctgcgcg tcgccaaagc ggccttgcgc aagctcctgg tgcttgcgcc cggtcgctcc
      181 tattgccagc cttgcttctc gccggtgtgg gacaccggtc ttgcttgtca cgcgctgatg
      241 gagtcgggcg gcgatgccgc cgatgcggcc gcgcggcgcg gcctcgactg gctcgccgag
      301 cgacagatcc tcgacgtcgc tggcgactgg gccgaatggc ggcccacgct ccggccgggc
      361 ggctgggcgt tccaatacgc caatccctat tatcccgatg tcgacgacac ggccgtcgtc
      421 gccatggcgc ttcatcgctg cgatggcgcg cgctatcgcg acgccatcga gcgcgcggcc
      481 gagtggatca tcggcatgca aagccgcaac ggcggctggg gcgccttcga tgccgacaac
      541 gtctattatt atctcaacta cattcccttc gccgatcacg gcgcgttgct cgatccgccg
      601 acggccgacg tctcggcgcg ctgtgtgggc tttctcgcgc aggttgacca cagctgcgat
      661 cctgccgtcg cccgcggcct cgactatctg ctgggcgagc aggagagcga cggctcgtgg
      721 ttcggccgct ggggcaccaa ctacatctac ggcacctggt ctgccctcct ggccctcaac
      781 gcggcgcgcg tcgacaaggc cgagccggcg atccgccgtg ccgtcgcatg gctggtggcg
      841 cgccagcgcc ccgacggcgg ctggggggag gacggcgcct cctattgggc cgaggcaccc
      901 cgcggcgagg gcaaggagag cacggcatcg cagaccgcct gggcgctgct cgcgctgatg
      961 gcggccggcg aggtcgacca cccgtccgtt gccaagggcg tgcgctatct gctggcgacg
     1021 cagcaggcgc atggcctttg ggacgagccc tggtacacgg cggtcggatt cccgcgcgtc
     1081 tttttcctga gctatcacgg ctatcccgcc ttcttcccgc tctgggcgtt ggcgcggtac
     1141 cgcaacctgc gccgaggcag cgaggaggcg ctcggctacg gcatgtgatg gccttcgcgg
     1201 ccgtcaccgg ccttgccgcc gaggcccgga tcgcccgacg cctcggcctc cgggccgtcg
     1261 ccacaggcgg tcgcgccgag cgcacggcag acgccgctgc ccgtctcatt gccgaagggg
     1321 cgacgggtct tctgagcttc ggcatctgcg gcggcctcga ccccgcgctc gcgtccggca
     1381 cgctcatcct gccgcgcgcg gtccagagcg agacgggcga gcgctatctc gccgatgccg
     1441 cggcgcatcg actctgcgcc gacgccctcg gcggcacgct cgtgacggag gacatgctgg
     1501 gcgccggcag catcgtcgac accgtcgcgc gcaaggccgc gctcttccgc cgcatggcgg
     1561 ccgtcgccat cgacaacgag agccatctcg tcgctgccgc ggcaaccgcg gccgggattc
     1621 catttttggt gctgcgcgcc gtcgccgacc ccgccacccg cgccttgccg cccgccgccc
     1681 tgatcggcct cgacgacgag ggccgccccg ccttgctccc ggtgctccgc tcgctcctcg
     1741 tgcagcccgg ccagctcggg gctctgctcg ccatcgcccg cgacacaagc cacgcgctcc
     1801 acgccctgag gagagtcgcc gcgatcgccc ccggcatctt ctctcgctga tccggcgact
     1861 gctgatcatc cctcgcgctc catcccctgc gcagatcatc ccctcccccc ttgaggggga
     1921 gggtcgggga ggggggtgcc accgccacag cggagcacag gagctgcttt cgtactccat
     1981 tgacgcatgg tgccttccgg ccccccgccc caaccctccc cctcaagggg ggagggggtc
     2041 gctttcgcct ttgcgcacgc gggcggcgtc accccgcgac agggtttctt aatcgctttt
     2101 gcctagcgta ttcgacccgc gcccggcatc gagaggacgg ctcgcccatg ttggcctgga
     2161 gagtccctgt ctacatcggt ggcgtgatcg ccatcgccgc cctcgtcgac ttcgcgctga
     2221 cgccgcaatc ggtctatgtc ttcaaaggcg tcgaggggcc gacggagaaa accgtcatcc
     2281 cgccgccggt cgccgcgtcg tggcgggaat accaggccgg cggcgccagc cgcctcgcca
     2341 tcctgctgac cgacgagagc tcgccctggc tcggcctcgc ccatggcctc aaatccatcg
     2401 gcgtgccctt caccatcacc gaggactatg ccgaggcggt gcgccatcgc gtcgtgatgg
     2461 tctatccgat gatctccggc cgcttgttgt cgccggcggc gctcaaggcg ctggccgagg
     2521 tgccggccaa tggcgggacg ctgatcggca gctatgtcct gggtggcggc ctcgaggacg
     2581 tcttcggcat cgacaaggcg ctgccctcgc agcgtcattt cgagatgcgc ttcgatcgtc
     2641 cggccgccga gcgcttcggt ctccgcgacc cgcgcgagct cgtcatcagc atcggcaatc
     2701 aggacaagac cgaagtcttc ggcacctacg gctatgtcgg cgtgacgggc acggcgcttg
     2761 ccagcttcga ggacggacag gcagcgatca ccgagcatcg tttggggtcg ggcagggctt
     2821 acgccctcgg catcgatctc ggcgagttgc tgatgcaggc ctacaacaat cgcgaggaag
     2881 ccttcagccg cgcttatgtg aacgagttcg agccatcgat cgacgtgctg ctgcgcgtca
     2941 tccgcgacat ctatcgcagc ggcgagccgc aggcggcgac gttgggcacg gtgccgttca
     3001 accgcccgct cgcggtcatg atcacccatg acatcgacta cacgcggtct ctgaacaacg
     3061 ccgtcgatta cgccacctat gagaagagcc agggcgtccg cgccacgtat ttcatgcaga
     3121 cgaaatacgt ccgcgactac aacgacgaga tcatgctgaa cgatgatacc gcgcccctgc
     3181 tgcgccggct cggcacgctc ggcatggagg tcggcagcca cacggtc
//
LOCUS       DASYNS010000075         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2360442, whole genome shotgun sequence.
ACCESSION   DASYNS010000075 DASYNS010000000
VERSION     DASYNS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2360442"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /gene="cobU"
                     /locus_tag="VGP50_02370"
     CDS             <1..436
                     /gene="cobU"
                     /locus_tag="VGP50_02370"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEV8026243.1"
                     /translation="AALYLATAEARDDEMAERIRRHRARRGARWTTIEEPLDLAPRLL
                     ELAAAPKPILVDCLTLWLANLLEAGRDVDREIAGLIEVLPRLPAAVVLVANEVGLGIV
                     PDNALARAFRDHAGRLNQAVAQRAERVVFMAAGLPLFLKDRN"
     gene            436..1446
                     /gene="cobW"
                     /locus_tag="VGP50_02375"
     CDS             436..1446
                     /gene="cobW"
                     /locus_tag="VGP50_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HEV8026244.1"
                     /translation="MRKLPATIVTGFLGAGKTSLIRHLLAHAGGRRIALVINEFGELG
                     IDREILLGCGVAGCSSEDVIELANGCICCTVADDFLPTITRLLERGAPPEHIVIETSG
                     LALPKPLVQAFAWPEVKTRVTVDGVIAVIDAAAVAAGRFADEAASGAAPAADHDNPLA
                     EVFDDQLACADLVLLNKSDLVAGEELARIERSMRGALRPAVKLLRATHGAVPPSVLLG
                     LAAAAEDDLAARPSHHDLDGAHDHEDFESFVVARGPVGDPQHFVTRLARMMSAHDILR
                     LKGFLDVPGKEFRQVVQGVGERVQHYFDRPWRADEARATRLVVIGRKGLDRAAIAVAL
                     QG"
BASE COUNT          216 a          553 c          539 g          254 t
ORIGIN      
        1 cgctgcgctc tatctcgcca ccgccgaagc gcgcgatgac gagatggcgg agcgcatccg
       61 tcgtcaccgg gcgcgccgcg gcgcgcgctg gacgacgatc gaggagccgc tcgatctcgc
      121 gccgcggctc ctcgaattgg cggcggcgcc aaagcccatc ctcgtcgatt gcctgacgct
      181 ctggctcgcc aatctgctcg aggccgggcg cgacgtcgac cgcgagatcg ctggattgat
      241 cgaagtgctg ccgcgccttc ccgcggccgt cgtgctcgtc gccaacgaag tgggtctcgg
      301 catcgtgccg gacaatgcgc tggcgcgcgc tttccgcgac catgccgggc gcctcaacca
      361 ggccgtcgcc caacgcgccg agcgcgtcgt cttcatggcc gccggcctcc ctctctttct
      421 caaggatcgg aactgatgcg caagcttccc gccaccatcg tcacgggttt cctcggtgcc
      481 ggcaaaacca gcctcatccg ccatttgctg gcccatgccg gagggcgccg catcgcgctc
      541 gtcatcaacg agttcggcga gctcggcatc gatcgcgaga tcctgctcgg ctgtggcgtc
      601 gcaggctgct cgagcgagga cgtgatcgag ctcgccaatg gctgcatctg ctgcaccgtc
      661 gccgacgact tcctgccgac gatcacccgg ctcctcgagc gcggagcgcc gcccgagcac
      721 atcgtcatcg agacctcggg cctcgccttg ccgaagccgc tggtccaggc cttcgcctgg
      781 cccgaggtaa agacacgcgt caccgtcgac ggcgtgatcg ccgtgatcga tgcggcggcg
      841 gtggcggcgg gccggtttgc cgacgaagcc gcgagcggtg ccgcgccggc cgccgaccac
      901 gacaatccgc tcgccgaagt gttcgacgac cagctcgctt gcgccgacct cgtgctgctg
      961 aacaagagcg atctcgtcgc gggcgaagag cttgcgcgca tcgagcgcag catgcgcggc
     1021 gcgcttcggc cggcggtgaa gctgctgcgc gcgacgcatg gcgcggtgcc gccgtccgtg
     1081 cttctcggac tcgcggcggc cgccgaggac gatctggcgg cgcgcccatc gcatcacgat
     1141 ctcgacggcg cgcacgatca cgaggatttc gagagcttcg tcgtggcgcg cggccccgtc
     1201 ggcgatccgc agcatttcgt gacgcggctg gcgcggatga tgtcggcgca tgacatcctg
     1261 cgcctcaagg gcttcctcga tgtgccgggc aaggagtttc gccaggtcgt gcagggcgtt
     1321 ggcgagcggg tccagcatta tttcgatcgt ccgtggcgcg cggacgaggc ccgcgcgacg
     1381 cggctcgtcg tcatcggccg caaaggcctc gaccgcgccg ccatcgccgt ggcgctgcag
     1441 gggtgagcgc tcccatgtcg agcggatttt tttcccctct cccctcgtgg gagagggagg
     1501 ggccagccgc gcagcggcgg gagggtgagg gggccacgcg cgcgatcatc ggagatgcgg
     1561 cc
//
LOCUS       DASYNS010000076         2251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4544354, whole genome shotgun sequence.
ACCESSION   DASYNS010000076 DASYNS010000000
VERSION     DASYNS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4544354"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VGP50_02380"
     CDS             complement(<1..196)
                     /locus_tag="VGP50_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026245.1"
                     /translation="MSKTDPGRFFEDFRLGDVLHHATPRTVGEADAALYLALTGTRFA
                     VNSADTFAQSIGLPRAPLDDF"
     gene            330..1055
                     /locus_tag="VGP50_02385"
     CDS             330..1055
                     /locus_tag="VGP50_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026246.1"
                     /translation="MSFALEFYSLSWDALKSALTQRKPELIAAVQAQQWDRLVNDTDL
                     VHNDNDAANADVTQGEDVVFADGFDEIAEAMAQKVPPGRDPADVGDNAALVVAAMIRH
                     LGKPVGAIQHAASVIHDKDGEVPMDFYGMFLDGVAGSAFNDHGLGDKLMARPLFGLFH
                     LDFLSWGGLTQAEIAALMPKYALTPATKQDEEWLAIADHAQGWLDTLVAALTAAATAK
                     SDLVTLYLTSEGSGDTFWDELRA"
     gene            1145..1942
                     /locus_tag="VGP50_02390"
     CDS             1145..1942
                     /locus_tag="VGP50_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026247.1"
                     /translation="MSFALECHALSWDALKAALTQRKPELLQAVEHQQWSTLLDSTDI
                     GQPDYHLLPYPHEEPGVHARSVPSDIRHGIDEIAEAMARKPSPNQDPPEISDNAALVF
                     AGIVRQMGQPVGTLRHDAAPAKDRDGELPIDFRALFLDGVAGSCFKDHGLGENLAARP
                     LFGLFHLDFLSWGGLTRQEIDGLLGNYALSDAERDDEEWQAIAGSAEAWLNQLVSALR
                     NAQAAKTDLVTLYRTVQGHHRSVWEEIAHEVRGGFFHRMSRPQRPAP"
     gene            complement(2001..>2251)
                     /locus_tag="VGP50_02395"
     CDS             complement(2001..>2251)
                     /locus_tag="VGP50_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEV8026248.1"
                     /translation="GFDGKTLIHPKTIAAANEAFAPASDEVAWARRIIAAHAEAEAAG
                     KGVVLVDGKLIENLHVEDARRTVALADAIAEMERAHAS"
BASE COUNT          382 a          771 c          733 g          365 t
ORIGIN      
        1 ggaaatcgtc gagcggtgcg cgcggcaggc cgatggattg cgcgaaggta tcggcggaat
       61 tgacggcaaa gcgcgtgccg gtgagcgcca gatagagcgc ggcgtcggcc tcgcccacgg
      121 tgcgcggcgt cgcgtgatgc aacacgtcgc ccaaacggaa atcctcgaag aagcggcccg
      181 gatcggtttt cgacatggat ggctccggca ggcggtcgac cgcatcttgt agagcggccg
      241 ggccgcgctg ttaaggccgc atgcccgcca tggcccaatt cgttaaggat ttctgagtac
      301 cctcgcatgc ctcgccgagg gagagatcca tgagcttcgc gcttgaattc tatagccttt
      361 cctgggacgc cctgaagtcg gcgctgaccc agcgcaagcc cgagctgatc gccgccgtgc
      421 aagcgcagca atgggaccgg ctggtcaacg acaccgacct cgtgcacaac gacaacgacg
      481 ccgccaacgc cgacgtgacg cagggcgaag acgtcgtctt cgccgacggc ttcgacgaga
      541 tcgccgaagc gatggcgcag aaggtaccgc ccggacggga tccggcggat gtcggcgaca
      601 acgccgctct cgtcgtcgcc gccatgatcc ggcacctcgg caagccggtc ggcgcgatcc
      661 agcatgcagc ctcggtcatc cacgacaagg atggtgaagt gccgatggat ttctacggca
      721 tgtttctcga cggcgtcgcc ggttccgcct tcaacgacca tgggctcggc gacaagctga
      781 tggcgcgtcc gctcttcggt ctcttccacc tcgatttcct gagctggggc ggcctgactc
      841 aggcggagat cgcggctctc atgcccaaat atgcgctgac gccggcgacg aagcaggacg
      901 aggagtggct ggccatcgcc gaccatgccc agggctggct cgacacgctc gtcgccgcct
      961 tgaccgccgc ggccaccgcc aagagcgacc tcgtcacgct ctatctcacc tcggagggaa
     1021 gcggcgacac gttctgggac gagctccgcg cctaggcgat ttctcgcgcc ggccgggccg
     1081 agaggaagtt cttaatgctc tcgggaccag tattcggtgc gcccgttttc tcgcggagag
     1141 accgatgagc ttcgcccttg agtgtcatgc gctttcctgg gatgccctca aagcggcgct
     1201 gacccagcgc aagcccgagc tcctgcaggc ggtcgagcac cagcaatggt cgacgctgct
     1261 ggacagcacc gacatcggtc agccggacta ccatctcctg ccctaccccc acgaagagcc
     1321 cggcgtgcat gcccggtccg tcccgtccga cattcgccac ggcatcgacg agatcgccga
     1381 ggcgatggcg cggaagccga gccccaacca ggatccgccg gaaatcagcg acaacgccgc
     1441 gctggtcttt gccggaatcg tccgccagat gggccagccg gtgggcacgc tccggcatga
     1501 cgctgcgccg gcgaaggacc gtgacggcga gctgccgatc gatttccgcg ccctgttcct
     1561 cgatggcgtg gccggctcgt gcttcaaaga ccatggcctc ggcgagaatc tggccgcgcg
     1621 gccgctcttc gggctgtttc acctcgattt cctgagctgg ggcggcctga ccaggcagga
     1681 gatcgacggc ctgctcggca attatgcgct gtccgatgcc gaacgcgacg acgaggaatg
     1741 gcaggcgatc gcgggctcgg cagaagcctg gctgaaccag ctcgtcagcg ccctccgcaa
     1801 cgcgcaggcc gccaagaccg acctcgtcac gctctatcgc acggtccagg gacatcaccg
     1861 gtccgtctgg gaagaaatcg cgcacgaggt tcgcggcggc ttcttccacc gcatgagccg
     1921 cccgcagcgg cccgcgcctt aagcgtcgcc cggcgtttca cgcgccgcga gcgccggcgt
     1981 ctcagtcacc cgatctctcg tcacgaggcg tgcgcgcgct ccatctcggc gatggcgtcg
     2041 gcaagggcga cggtgcgccg cgcgtcctcg acatggagat tctcgatcag cttgccgtcg
     2101 accagcacca cgcccttgcc ggcggcctcg gcctcggcat gggcggcgat gatgcgccgc
     2161 gcccaggcga cctcgtcgct cgccggcgca aaggcctcgt tggcggcggc gatcgtcttc
     2221 ggatggatga gagtcttgcc gtcgaaaccc a
//
LOCUS       DASYNS010000077         3640 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1891950, whole genome shotgun sequence.
ACCESSION   DASYNS010000077 DASYNS010000000
VERSION     DASYNS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891950"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /gene="parA"
                     /locus_tag="VGP50_02400"
     CDS             <1..618
                     /gene="parA"
                     /locus_tag="VGP50_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HEV8026249.1"
                     /translation="KGGAGKTTLAAHLALAWAGKRSVSVIDIDPQGSLAAWFRLREQQ
                     AGTPGAALDVAAVTGWRVSSEVERQARSHDIVLIDSAPRAETEARLAVRASSLVLIPV
                     QPSPMDVWATRATLELARQERVPALLVLNRVPSRANLTQIMAAKLAELGVPVAATKIG
                     NRVALAAALAEGQGILEAAPGSRAAEEITALAAEVLEHMASRVAA"
     gene            863..2104
                     /locus_tag="VGP50_02405"
     CDS             863..2104
                     /locus_tag="VGP50_02405"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8026250.1"
                     /translation="MSSNSVLSSPRLFHGWLVVAGAFAVTFVGFGSAYTFSAFLAPLQ
                     KDFAASRGSVSLVFSLAGFLYFGLGIISGPLADRWGSRPLAVAGMILIGLGLALASAA
                     RSLAEVYAAYGLGVGLGVGCSYVPALGAVQRWFTRRRGFASGLAVSGIGVGTLVMPAL
                     ASLLIAAWGWRQAYLVLGGLAAVLGAGMALMIENDPRDRGLGPDGDPPQSAAPSTLPA
                     GATVREAIRSRVFVSLYGACLICSFGVFVPFVHLVPYAMDHGVPPASAALLLGVIGIG
                     STAGRFFLGGLADRMGRRHALLTMIIGMALALAVWAVATDFWSLAVFAFAYGVFYGGW
                     VAVLPAVVMDYFGGRNVSAIIGILYTSVAFGTLIGPSAAGFAFDLSHSYTLPILAGIA
                     ANILAAGVVAGMSRIRAAAGG"
     gene            complement(2112..2852)
                     /locus_tag="VGP50_02410"
     CDS             complement(2112..2852)
                     /locus_tag="VGP50_02410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV8026251.1"
                     /translation="MIEKQLDIPAKDGGIDTFICHPERRGPWPAVIFYMDAPGIREEL
                     RDMARRLATVGYYVLLPNLYYRAGARSLIDTGALGEGSPERKRMFDLMQSLTNGLITD
                     DTRHLLAFMDQQREIRKGPVGCVGYCMSGRFVVTVAGRFPDRFAAAASVYGVGLMTDQ
                     PDSPHLLARDIKGELYFACAETDRYAPLSMLGALKTEFERAGVNFEIEMYPGTEHGFA
                     FPQRYCYDKAAAETHWERLFDLFRRRLG"
     gene            complement(2887..3636)
                     /gene="cysQ"
                     /locus_tag="VGP50_02415"
     CDS             complement(2887..3636)
                     /gene="cysQ"
                     /locus_tag="VGP50_02415"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEV8026252.1"
                     /translation="MLELADAAAMEAGAAILRVYAGDFTVEQKADASPVTLADQLAEA
                     VVLAKLGAATPEIPVIAEERAAKHGLPGAAPPLFWLVDPLDGTKEFIKRNGEFTVNIA
                     LIERGRAVLGVVHVPAQGVTYAASGTGTATRRRGNGPAEPIAARALPVRRPIVVHSRS
                     HADEQRIAQYVAALPEAECRVAGSSVKFCLIAAGEADLYPRFGPTMEWDTGAAQAVLE
                     AAGGSVTTLDGAPLRYGKPGFINPDFIARGR"
BASE COUNT          502 a         1228 c         1279 g          631 t
ORIGIN      
        1 aagggtggtg ccggcaagac gacgctcgcc gcccatctgg cgctcgcctg ggctggcaag
       61 cggagtgtct ccgtcatcga catcgacccg cagggcagcc tcgccgcgtg gttccgcttg
      121 cgggagcagc aagcgggcac gcccggcgcc gcgctcgacg tcgcagccgt caccggctgg
      181 cgcgtctcga gcgaggtcga gcgccaggcg cgcagccacg acatcgtcct catcgacagc
      241 gcgccgcgtg ccgagaccga ggcgcggctg gcggtgcggg cctcgagcct ggtgctgatc
      301 ccggtgcagc cctcgcccat ggatgtgtgg gcgacacgcg cgacccttga gctcgcacgg
      361 caagagcgcg tgccggcgct cctcgtcctc aaccgcgtgc cctcgcgcgc caacctcacg
      421 cagatcatgg cggcaaagct cgccgagctg ggtgtgccgg tggcggcgac gaaaatcggc
      481 aatcgcgtcg ccctcgccgc ggcgctcgcc gaagggcagg gcatcctcga agccgccccc
      541 ggcagccgcg ccgccgaaga gatcacggcg ctcgccgccg aagtcctgga gcacatggcg
      601 tcccgcgtgg cggcgtagcg ttacccctca ccctcccagc gctacgcgct gggcccctcc
      661 ctctcgcaca gaggggagag ggagaagagg cgttcggtgg cggcagccga tgcgccaccc
      721 tgtggttttc ctgatctgga acgcgcagaa cgtccggctt caaggtccgt ttttcgccag
      781 cgaaatatcg tgcatcggac caccctgtgg ccttgaacgc atctctggcg gctgagcaca
      841 gcgaattgaa cgaggactcc ggatgagcag caactcggtg ctgtccagcc cgcgattgtt
      901 ccatggctgg ctcgtggtgg ccggcgcttt tgccgtgacc ttcgtcgggt tcgggagcgc
      961 ctatacattc agcgccttcc tcgcgccgct gcagaaggac ttcgccgcgt cgcgcggctc
     1021 ggtttcgctg gtcttctcgc tggcgggctt tctctatttc ggccttggca tcatcagcgg
     1081 cccgctcgcg gaccgctggg ggtcgcgccc tctggccgtc gccggcatga tcctgatcgg
     1141 gctggggctg gcgctcgcca gcgccgcgcg cagcctggcg gaagtctatg cggcctacgg
     1201 actcggcgtg ggtctgggcg tcggctgctc ctatgttccg gcgctgggcg ccgtgcagcg
     1261 ctggttcacg agacgccgcg gctttgcgtc gggcctggcg gtcagcggca tcggtgtcgg
     1321 cacgctggtc atgccggcgc tggcctcgct cctcattgcc gcatggggct ggcgccaggc
     1381 ctatctcgtc ctcggcgggc ttgccgccgt cctgggcgcg ggaatggcgc tcatgatcga
     1441 gaacgatccg cgtgatcgcg ggctcgggcc cgacggcgat ccgccgcaat ccgctgcacc
     1501 gtccacgctc ccggccggcg ccacggtgcg cgaagcgata agatcacgcg tgttcgtcag
     1561 cctctacggc gcctgcttga tctgctcatt cggcgtgttc gtgccgttcg tccacctcgt
     1621 tccctacgcg atggatcacg gcgtgccgcc ggcttcggcc gctcttctgc tgggggtgat
     1681 cggtatcggg agcaccgccg ggcgattctt tctcggcggc ctggccgacc ggatgggccg
     1741 gcgacacgcg cttctgacga tgatcatcgg catggcgctc gccttggccg tctgggcggt
     1801 ggcgacggat ttttggtcgc tggccgtctt tgccttcgcc tatggcgtct tctacggcgg
     1861 atgggtcgcc gtccttccgg ccgtcgtcat ggattatttc ggcggccgca atgtgagcgc
     1921 catcatcggc attctctaca cgagcgtggc gttcggcacg ctgatcggcc cgagcgccgc
     1981 cggattcgcg ttcgatttga gccacagcta cacgctgccc atcctcgccg gcatcgccgc
     2041 gaacatcctc gctgcgggcg tcgtcgccgg catgtcgcgg atacgcgcag cggcgggagg
     2101 gtgaggggcc gttacccgag acgccggcgg aagagatcga aaagccgttc ccaatgagtc
     2161 tcggcggccg ccttgtcgta gcagtagcgc tgcgggaagg cgaagccgtg ctcggtgccg
     2221 ggatacatct cgatctcaaa attcacgccg gcacgctcga actccgtttt gagcgcgccc
     2281 agcatcgaga gcggcgcgta gcggtcggtc tcggcgcagg cgaaatagag ctcgcccttg
     2341 atgtcgcgcg cgagaagatg ggggctgtcg ggctgatcgg tcatgaggcc gacgccatag
     2401 acggaggccg cggcggcgaa gcggtcggga aagcggccgg cgaccgtgac gacgaagcgc
     2461 ccgctcatgc agtagccgac gcagccgacg ggtcctttcc tgatctcgcg ctgctgatcc
     2521 atgaaggcga ggagatgccg cgtgtcgtcg gtgatgagcc cattggtgag gctctgcatc
     2581 aggtcgaaca tgcgcttgcg ctcggggctg ccttcgccga gagcgcccgt gtcgatgagg
     2641 ctgcgcgcgc cggcgcggta atagagattg ggcagcagca cgtaatatcc gacggtggcg
     2701 agccggcgcg ccatgtcgcg cagctcctcg cggatgcccg gcgcatccat gtagaagatg
     2761 acggcaggcc agggaccgcg gcgctcggga tgacagatga aggtgtcgat gccgccgtcc
     2821 ttcgcgggaa tgtcgagctg cttttcgatc atcgggacgt cctcctctgt cttttccgcg
     2881 tggggctcac cgcccgcgcg cgatgaaatc ggggttgatg aagccgggct tgccgtagcg
     2941 caagggcgcg ccgtcgagcg tcgtcacgct gccgccggcc gcttcgagca ccgcttgggc
     3001 cgcgcccgtg tcccactcca tcgtcggccc gaaccgcgga tagagatcgg cctcgcccgc
     3061 ggcgatgaga cagaatttga cggagcttcc agcgacgcgg cattccgcct cggggagcgc
     3121 cgcgacatac tgcgcgatgc gctgctcatc ggcatgcgag cggctgtgga ccacgatcgg
     3181 ccggcgcacc ggcagcgcgc gcgcggcgat gggctcggcc ggtccgttgc cgcgtcgccg
     3241 cgtcgcggtg ccggtgccgg aagcggcgta ggtcacgcct tgcgccggca catgcaccac
     3301 gccgagcacg gcacggccgc gctcgatgag cgcgatgttg accgtgaact cgccgttgcg
     3361 cttgatgaac tctttcgtgc cgtcgagcgg atcgacgagc cagaaaagcg gcggcgccgc
     3421 gccggggagg ccgtgtttcg cggcgcgctc ctcggcgatg acgggaatct ccggcgtcgc
     3481 cgcgccgagt ttcgccagca cgacggcttc cgcgagctgg tcggcgagcg tcaccggcga
     3541 tgcgtcggct ttctgctcga cggtgaagtc gccggcatag acgcgcagaa tcgccgcacc
     3601 ggcttccatc gccgcggcgt cggcaagctc gagcaagccg
//
LOCUS       DASYNS010000078        12153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5168283, whole genome shotgun sequence.
ACCESSION   DASYNS010000078 DASYNS010000000
VERSION     DASYNS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5168283"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1238)
                     /locus_tag="VGP50_02420"
     CDS             complement(165..1238)
                     /locus_tag="VGP50_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8026253.1"
                     /translation="MDKIEPSEAIDHRRRRFFGAAALTVAAAQLGVIAPADAQSGDAK
                     KPAALPEIKRGTNTAFPALKQIDAGVLTVGYAEAGPADGPPVILLHGWPYDIHSYVDV
                     APLLASSGYRVIVPYLRGYGTTRFLSAETFRNGQPSAVAVDIVALMDALKIDKATLGG
                     FDWGARTANIIAALWPERCKAMVSVSGYLIGGQEAGKVPLPPSAELQWWYQFYFATER
                     GEAGYDKYRHDFAKLIWQLASPKWNFDAATFDRSAAAFANPDHVAISIHNYRWRLDLA
                     KGEPKYDDLEQRLAAGPVIGVPTITLEGDANGAPHPDPASYAKKFSGKYAHRLIKGGI
                     GHNLPQEAPQAFAEAVVEVAAEA"
     gene            complement(1619..2287)
                     /locus_tag="VGP50_02425"
     CDS             complement(1619..2287)
                     /locus_tag="VGP50_02425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV8026254.1"
                     /translation="MAQTITLTAKDGHTLAAYRADPVGKPKGALVVLQEIFGVNSHIR
                     RVTDDFAAEGYVALAPALFDRAERGVELGYDPASIASGRKIREQVALEGTLADLQAAI
                     DQVQPFGKVGVVGYCWGGSLAFLAATRLQGVAAAVGYYGGMIAAHAEEHPKAPVLLHF
                     GETDQSIPMSDVAKVQKARPDVAIHVYKAGHGFNCDERGSYDAASAKLALTRTLAFFA
                     ETLT"
     gene            complement(2325..3425)
                     /gene="ychF"
                     /locus_tag="VGP50_02430"
     CDS             complement(2325..3425)
                     /gene="ychF"
                     /locus_tag="VGP50_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV8026255.1"
                     /translation="MGFNCGIVGLPNVGKSTLFNALTATAAAEAANYPFCTIEPNIGR
                     VAVPDDRLEVCAGLARSAKIVPTQLEFVDIAGLVRGASRGEGLGNQFLGHIRAVDAIA
                     HVLRCFEDGTVTHVEGSIDPIRDAETVETELLLADLESLERRVAATQKKARGNDKEAK
                     AQLQVMEPVLAALAAGKPARSVVVLPEQRPIFRSLQLLSAKPVLFVCNVEESAAATGN
                     AQSARVADYAAAQGAASVVISAAIEAEVAQLADPQEKRDFLEGLGLAESGLARVIGAG
                     YALLDLVTFLTVGPKEARAWTVARGTKAPQAAGVIHSDFEKGFIRVETIAYDDFVAGG
                     GEQGAKDAGKMRLEGADYVVQDGDILHFRFNV"
     gene            complement(3427..4050)
                     /gene="pth"
                     /locus_tag="VGP50_02435"
     CDS             complement(3427..4050)
                     /gene="pth"
                     /locus_tag="VGP50_02435"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV8026256.1"
                     /translation="MPLRLIVGLGNPGARYERNRHNIGFMAADEIVRRHRFGPFRAKF
                     DAAIAEGNIAGDRVYLAKPQTYMNASGDAVGAARRFYKIDSSEIAVIYDEIDLAPGKL
                     RVKQGGGSAGHNGIRSIEGAIGEDFWRVRLGVGHPGDKRLVETYVLQNFDAEEMQWVT
                     SLVDAVAEALPLHVAENASGFMTKVALILKPPPPKPPRAAEDKENKD"
     gene            complement(4312..5091)
                     /locus_tag="VGP50_02440"
     CDS             complement(4312..5091)
                     /locus_tag="VGP50_02440"
                     /inference="COORDINATES: protein motif:HMM:NF004128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEV8026257.1"
                     /translation="MADMITIRAASRDRAGKGPARAARRAGRVPGVIYGEKQPPVLIS
                     IDPRELTTHLSKRGFFARLVNVELDGQSFRTLPRDVQLNPVTDVPEHVDFMRVGAATR
                     LTVAVPVIFLNQEKSPGIRRGGILNIVRHEIDLVCTVDDIPERITIDLDGLDIGDSVH
                     ISAVTLPSGTRPAISGRDFTIASVAAPTAVREEQAAAAAAAAAAAAAAAAAPEGGVPG
                     APAVPGAAPGAAAPGAAPAAGATPAAGAKEAAGDGKGKGKG"
     gene            complement(5201..6133)
                     /locus_tag="VGP50_02445"
     CDS             complement(5201..6133)
                     /locus_tag="VGP50_02445"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEV8026258.1"
                     /translation="MKILTCNSNRPLAEAISAYLATSLTNASVRRFSDMEVFVEIHEN
                     VRGEDVFVIQSTSYPANDNLMELLVTLDALKRGSARRTTAVLPYYGYARQDRKSGPRT
                     PISAKLVANMITAAGADRVLTLDLHAGQIQGFFDIPTDNLIAAPVFVKDIKERYDGAD
                     LVIVSPDVGGVVRARQLARRLDADLAIIDKRRERAGVSEVMNIIGDVDGRRCILIDDI
                     VDSAGTLCNAAEALKERGAKSVSAYVSHGVLSGGAVARVSASPMEMLVTTDSILATEA
                     MRIAKNIRQLSIAPLMAEAIRRISDERSVSSLFD"
     gene            complement(6234..7034)
                     /locus_tag="VGP50_02450"
     CDS             complement(6234..7034)
                     /locus_tag="VGP50_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026259.1"
                     /translation="MLRLIAFLPLLLAACQPLPHPFADDRPDSSLLAPRDSAGVRVEP
                     LPGTPSDLAEGIAAALREREIPASTQGRNKRSFELRLAAGGPPEGSEAAFRVDWELRS
                     ADGRSLGHGSAAGGDARSVAAVAAPEIARLVQDEPPAMAGSSEPVVALRPVTGAPGDG
                     GRTLTRAMDYALRHANVPLTDRPGAESFVLTGTVALSPPAAGQQQVKVHWALLKPDGR
                     EIGRVDQENAVPAGSLDGAWGDIAYAVASAAAPGVAALIARARAEIGS"
     gene            complement(7198..8793)
                     /locus_tag="VGP50_02455"
     CDS             complement(7198..8793)
                     /locus_tag="VGP50_02455"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEV8026260.1"
                     /translation="MSASAAERAVSTISAISTVADVTRHHARERPDQTALYFDGERIS
                     YGVLDRRASQVAQGLIRAGIEREARVAVLAKNAPEFFELWFGAAKAGVVLVPVNFRLA
                     PPEIAYVVNDARADLLFVGPDYYDVIDKVAAELTSVREIVALDGQHPGWIDYPRWRDR
                     ENASDPMRPLAGLDCAIQMYTSGTTGHPKGAQLSHDNLLTLLPGALRQWGVWRDGDVN
                     LVCMPLFHIGGSGWALIGLYRGVENVLTRDFDALTILRLIAERRITKTLFVPAMMLFL
                     LQAPQCRATDFSSLELIVYGASPAPLDLLRNARAVFGCGLAQVYGLTETTGAITYLPP
                     EDHGEHAIERMKSCGKPADGVEIGIVDALGKKLPPGEVGEVVCRTPQVMLGYWNLPDA
                     TSRAIRDGWFHTGDAGYLDKEGYLFIYDRVKDMIISGGENIYPAEVESALFGHPAIAD
                     VAVIGVPDEQWGEAVKAIVVKKPGVAVSAKELIDYARERIAGYKVPRSVDFIDALPRN
                     PSGKILKRELRKPYWLGHERQVN"
     gene            complement(8899..9540)
                     /locus_tag="VGP50_02460"
     CDS             complement(8899..9540)
                     /locus_tag="VGP50_02460"
                     /inference="COORDINATES: protein motif:HMM:NF040011.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6151 family protein"
                     /protein_id="HEV8026261.1"
                     /translation="MGKNAALQCRCGAVRGVVANAAPDAVNRAVCYCDDCQAYLHHLG
                     RTDLFDAHGGTDVVQVAPAALSFVQGKDRIQGLRLSPKGLYRWYASCCKTPLGNTLGP
                     AMPFVGIVARVFDSASQKADDVFGPPLGAVHGKFAVGDAPEGSTGFKARLIARMLRKI
                     LGWRLTGRTWPHPFFDRATREPIYPLTTLSRAEREALRPLCGPRPSAAQGRTG"
     gene            complement(9605..12004)
                     /locus_tag="VGP50_02465"
     CDS             complement(9605..12004)
                     /locus_tag="VGP50_02465"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8026262.1"
                     /translation="MSDFTTAQTIFQSAISALNLRRFDDAERLFKTMLAKEPGNFAAL
                     NLLTAVLMSRGRFAEAEAFITRALKINQGSAASFYNYGIILKMLGKPRPALEQFDRAL
                     ALDPNACDAWNSRGTVRNDLKDYDRAIADFDKALSIDPNCDGALCNKGKSLSLLKRHD
                     EALAAYDSALRLKPDLLEAWLGRGTVLREQQLRGEALAAYIKALALAPDAAEARLGRA
                     FVLADLNRSGEALADYDKALQLKPGLAGGWNGRGNLLAELKRFDEALAAYDRALAVKP
                     DLAEAWLGRGNVFRELKRYEDAFAAYDKALAAKPDFAEARVCRADGLRMLGRYAEATA
                     DYEKALALAPDLPGIEGMRLHCKMLLCDWRDVAAEREHLIAAVRQKKANATPFAFYNL
                     SASPEDQLDCARSWSAAKYPALAPRIWQGPIYRHERIRVAYVSADFHQHATAYLAAGL
                     FECHDKSRFDITAISVGQDDASEMRGRLKQAFDQFIEARALSDDAIAERVRSAEIDIL
                     VDLKGFTQDARTGVFARRPAPIQVNYLGFPATMGADYIDYLIADRIVVPASHKAHYAE
                     KIAYVPGSYQINDAKRPISGRSFARAELGLPDTGFVFCCFNNNFKIFPDVFACWMRLL
                     KQREDSVLWLLEDNAGAAANLRQEARMSGVDPDRLVFAKRAALPEHLARHRAADLFLD
                     TLPYNAHTTASDALWAGVPVLTQIGETFAGRVAASLLRAVGLPELITETRGAYEQRAL
                     DLATHPAELSRLREKLAQTRLSAPLFDTRSSTRHLERAYLAMYERYQAGLPPDHIDDG
                     G"
     gene            complement(12043..>12153)
                     /locus_tag="VGP50_02470"
     CDS             complement(12043..>12153)
                     /locus_tag="VGP50_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HEV8026263.1"
                     /translation="GGDTAAEPDRFFSYRRACLRKETDYGRALSAICLAE"
BASE COUNT         1945 a         3908 c         4308 g         1992 t
ORIGIN      
        1 aggctcgcga agatgctatt cacgtaccgc atgcccggtt tcctttctga gtctcgacaa
       61 ccagaaagta cacggcttgt gccgatgtgg ccgggcatgc gcctgcgact tatcgactca
      121 ttccccggac agccctgcgc ggtgcgggag aggggctata cagatcaagc ttcggccgcc
      181 acctcgacca cggcctcggc gaaggcctgc ggggcttctt gtggcagatt gtgtccgatg
      241 ccgcccttga tcaggcggtg ggcatatttg cccgaaaatt tcttggcata ggaagcgggg
      301 tccgggtgtg gcgcgccatt ggcgtcgcct tcgagggtaa tcgtgggtac gccgatgacc
      361 gggcccgcgg ccagccgctg ctccagatcg tcatatttgg gctcgccttt tgcgaggtcg
      421 agccgccacc ggtaattgtg gatggaaatg gcgacatgat ccggattggc aaaggccgcg
      481 gcgctacggt cgaaagtggc agcgtcgaag ttccacttcg gcgaggcgag ctgccagatg
      541 agcttcgcga agtcgtgccg gtacttgtca tagccggcct cgccgcgttc cgtcgcgaaa
      601 tagaattggt accaccattg gagctcggcg gatggcggca acggcacctt gccggcttcc
      661 tggccgccga tcaggtagcc gctgacggag accatggcct tgcagcgctc gggccagagc
      721 gcggcgatga tgttggcggt ccgcgctccc cagtcgaagc cgccgagcgt ggccttgtcg
      781 atcttcagcg catccatcaa ggcgacgatg tcgacagcga ccgccgaggg ctggccgttc
      841 cggaacgtct cggccgagag aaagcgcgtc gtgccatagc cgcgcagata ggggacgatc
      901 acgcggtagc cgctcgacgc cagcagcggg gcgacgtcga catagctgtg aatatcgtag
      961 ggccagccgt gcagaagaat gaccggcggt ccgtcggcgg gaccggcttc ggcgtatccg
     1021 acagtgagga cgccggcgtc gatctgcttc agcgcgggga acgcggtatt cgttccccgc
     1081 ttgatctccg gaagcgccgc cggttttttc gcgtcgccgg attgcgcgtc tgccggagcg
     1141 atcacgccga gctgagcggc ggcaaccgtc agggcagccg cgccgaaaaa gcgccgccga
     1201 cgatggtcga ttgcttcgga gggttcgatc ttgtccatgg tcagtctcct tcgtgacggc
     1261 ggcgaacggc gggaaagcga taccggcggg cgatgacaat gtgcggacac gatcactcgc
     1321 ttcggcggaa gcgagcctcg ccgcaacaga atggtccacc ccggtgggtg ccatcggaaa
     1381 aagtttagag atcagtggcg gaaaacgtcg ctgtctccgg ccgcacggtc ttgcgaacga
     1441 gatagtccag ccgcgctcga ttccgatcaa ttatacgatc ggccaaggag ggccaccaca
     1501 agcgatagga tgcgtcgcgc atctttgcgt cgactcatcc ggcaggcaga gcgttcagtg
     1561 gcgaccgacc cctcaccctt ccgcggtgcg ggagaggggt ttttgcgcgg gctcgtcgct
     1621 aggtcagcgt ctcagcgaag aaggcgaggg tgcgggtgag ggcgagcttg gcgcttgcgg
     1681 cgtcgtagct gccgcgctcg tcgcagttga agccgtggcc ggctttgtag acgtggatcg
     1741 ccacgtcggg gcgggccttc tgcactttcg cgacgtcgct catcggaatg ctctggtcgg
     1801 tctcgccgaa atgcagcagc accggcgctt tcgggtgctc ctcggcatgg gcggcgatca
     1861 tgccgccgta atagccgacc gcggcggcga caccttggag gcgcgtcgcc gcgaggaagg
     1921 cgagcgagcc gccccagcaa tagccgacga cgccgacttt gccgaagggt tgcacctgat
     1981 cgatcgcggc ctgcaggtcg gcgagcgtgc cttcgagcgc cacttgctcg cggatcttgc
     2041 gtccgctcgc gatgctggcg gggtcatagc cgagctcgac gccgcgctcg gcgcggtcga
     2101 agagcgccgg cgccagcgcg acatagcctt ctgcggcgaa gtcgtcggtg acgcggcgga
     2161 tgtggctgtt gacgccgaag atctcttgaa ggacgacgag cgcgcccttg ggcttgccca
     2221 cgggatcggc gcgatacgcg gcgagcgtgt ggccgtcctt ggcggtgagc gtgatcgtct
     2281 gtgccatctg cggtcctccc tccggcagcg aatgcgaaat tgaatcagac attgaagcgg
     2341 aaatgcagga tatcgccatc ctgcacgaca tagtcggcgc cttcgaggcg catcttgccg
     2401 gcgtccttgg cgccctgctc gccgccgccg gcgacgaaat cgtcataggc gatggtctcg
     2461 acgcggatga agcctttctc gaagtcgctg tgaatgacgc cggcggcctg cggcgccttg
     2521 gtgccgcgcg ccacggtcca ggcgcgcgcc tccttggggc cgacggtgag gaaggtgacg
     2581 agatcgagga gcgcgtagcc ggcgccgatg acgcgggcga ggccggactc ggcgaggccc
     2641 aatccctcga ggaagtcgcg cttctcctgg ggatcggcaa gctgcgccac ttcggcttcg
     2701 atggcggcgg agatgacgac gctcgccgcg ccttgcgcgg ccgcatagtc ggcgacgcgg
     2761 gccgattgcg cattcccggt cgccgccgcg ctctcctcga cattgcacac gaagaggacg
     2821 ggcttggcac tcagaagctg caggctcctg aagatcggcc gctgctccgg cagcaccacc
     2881 acgctgcggg cgggcttgcc ggcggcgagc gcggccagca ccggctccat cacctgaagc
     2941 tgcgccttcg cttccttgtc gttgccgcgc gcctttttct gagtcgcggc gacgcgccgc
     3001 tcgaggctct cgagatcggc gagcaggagc tcggtctcca ccgtctcggc atcgcggatc
     3061 gggtcgatcg agccctcgac atgcgtgacg gtgccatcct cgaagcagcg cagcacatgg
     3121 gcgatggcgt cgacggcgcg gatatgaccg aggaactggt tgcccaggcc ctcgccgcgg
     3181 gaggcgccgc gtacgaggcc ggcgatgtcg acgaattcga gctgcgtcgg cacgatcttg
     3241 gccgagcggg cgagcccggc gcagacctcg aggcggtcat ccggcaccgc gacgcggccg
     3301 atattcggct cgatggtgca gaagggataa ttcgccgcct cggccgcagc ggtcgcggtc
     3361 agcgcgttga agagcgtcga cttgcccaca ttgggaaggc cgacgatgcc gcaattgaaa
     3421 cccatgtcag tccttgttct ctttgtcttc ggcggcgcgg ggcggcttgg gcggcggcgg
     3481 cttcaggatc agcgccactt tggtcatgaa gcccgaggca ttctcggcga cgtgcagcgg
     3541 cagcgcctcg gcgacggcgt cgaccaaaga cgtcacccac tgcatctcct cggcgtcgaa
     3601 attctgcaac acataggtct cgacgaggcg cttgtcgccg ggatggccga cgccgagccg
     3661 cacgcgccag aaatcctcgc cgatcgctcc ttcgatggag cggatcccgt tgtggccggc
     3721 cgagccgccg ccctgcttga cgcggagctt gcccggcgcg aggtcgatct cgtcatagat
     3781 gacggcaatc tccgaggaat cgatcttgta gaagcgccgg gcggcgccca ccgcgtcgcc
     3841 cgaggcgttc atgtaggtct gcggcttcgc caggtaaacc cgatcgccgg cgatattgcc
     3901 ctcggcgatg gcggcgtcga acttggcgcg aaaggggccg aagcgatggc ggcggacgat
     3961 ctcgtccgcc gccatgaagc cgatgttgtg ccggttgcgc tcgtagcgcg cgccgggatt
     4021 gccgagcccg acgatgaggc ggaggggcaa cgcggcgtcc taattagtgt tgagcctgag
     4081 agacggtgag aacacccccc acggatcgcg tcatccgaga cacatccccc tctcccccac
     4141 cgggggagag ggtcggggtg agtgggcgtg cagcagagat ggtgccgcgc ggcgccaggc
     4201 gccgatcccg caacgaccca tgtcgatcgc ccgtcgcgcg ccgccccctc acccttccct
     4261 ctctcccgat gggggagagg gggagatgtg tcgaaggcga taccaccctg ctcagccctt
     4321 gcctttgccc ttgccgtcgc cggcggcttc tttcgctccg gcggcggggg tggcgccggc
     4381 ggcgggcgcg gcgccggggg cggcggcacc gggagcggca cccggtacag ccggggcgcc
     4441 cggcacgccg ccttcgggag cggcggctgc tgccgctgcg gcggcagcgg cggcggctgc
     4501 agcagcggcc tgctcctcgc gcaccgcagt gggcgcggcg acggaggcga tggtgaagtc
     4561 gcgcccggag atggcggggc gcgtgcccga cgggagcgtc acggcgctga tgtgcacgct
     4621 gtcgccgatg tcgaggccgt cgagatcgat cgtgatgcgc tcggggatgt cgtcgacggt
     4681 gcagacgagg tcgatctcgt ggcgcacgat gttgagaatg ccgccgcggc ggatgcccgg
     4741 cgatttctcc tgattcaaga agatcacggg cacggccacg gtgaggcgcg tcgcggcgcc
     4801 gaccctcatg aaatcgacat gttcgggaac gtcggtcacc gggttgagct gcacgtcgcg
     4861 cggcagcgtg cggaacgact ggccgtcaag ctcgacattg acgaggcggg cgaagaagcc
     4921 gcgcttgctc agatgcgtcg taagctcgcg cggatcgatg ctgatgagca ccggcggctg
     4981 cttctcgcca tagatgacgc ccggaacccg gccggcacgt cgcgcggccc gggccggccc
     5041 cttacccgcc cggtcgcgcg aagcggcgcg gatggtgatc atgtcggcca tctctccaac
     5101 tccttcttca gaatgcggac cggcgcgtcg ctcgcgccgg tttcaccgct tgccggcgcc
     5161 ggcctccagg ggtgccgcgc gccgggaatc atgtgcaatc ctaatcgaac aggctcgaga
     5221 cggagcgctc gtcgctgatg cgccggatcg cctcggccat gagcggcgcg atgctgagct
     5281 ggcggatgtt cttggcgatg cgcatcgctt cggtggcgag gatgctgtcg gtcgtcacca
     5341 gcatctccat gggcgacgcg ctcacccgcg ccacggcgcc gcccgagagc acgccatggc
     5401 tcacataggc ggagacggat ttggcgccgc gctccttgag cgcctcggcg gcgttgcaca
     5461 aggtgccggc gctgtcgacg atgtcgtcga tgaggatgca gcggcggcca tcgacgtcgc
     5521 cgatgatgtt catgacctcg gagacgccgg cgcgctcgcg ccgcttgtcg atgatggcga
     5581 ggtcggcgtc gaggcggcgc gcgagctggc gcgcgcgcac gacgccgccg acatcgggcg
     5641 acacgatcac gagatcggcg ccgtcgtagc gctccttgat gtccttcacg aagaccggcg
     5701 cggcgatgag gttgtccgtc ggtatgtcga agaaaccctg gatctggccg gcgtgaaggt
     5761 cgagcgtcag cacgcggtcg gcgcccgccg cggtgatcat gttggcgacg agcttcgccg
     5821 agatcggcgt gcgcggcccc gatttccggt cctggcgcgc atagccgtaa tacgggagga
     5881 ccgccgtggt gcgccgcgcc gagccgcgct tcagcgcgtc gagcgtgacc agcagctcca
     5941 tgaggttgtc gttggccggg taggacgtcg actggatgac gaagacatcc tcgccgcgga
     6001 cgttctcgtg aatctcgacg aacacttcca tgtccgagaa gcgccgcacg ctggcattgg
     6061 tgagcgacgt cgcgagatac gcggagatgg cctcggcgag cggccgattg ctgttgcacg
     6121 tcaggatttt catacggtct cgccggtctg cggcccgctc ggaggcccct gcccgagcgc
     6181 gcgggattgt aacaaactac tactcagttg agcaatagca ccggagcggc gcgtcaggag
     6241 ccgatttccg ccctggcgcg ggcgatgagg gcggcgacgc ccggggcggc ggcgctggcc
     6301 acggcatagg cgatgtcgcc ccaggcgccg tcgaggctgc cggctgggac ggcattctcc
     6361 tggtcgaccc ggccgatctc ccggccatcg ggcttgagca gcgcccaatg caccttcacc
     6421 tgctgctggc cggcggcggg cggcgagagg gcgacggtgc cggtgaggac gaagctttcc
     6481 gcgccgggcc ggtcggtgag cgggacgttg gcatggcgga gcgcgtagtc catggcgcga
     6541 gtgagcgtgc gtccgccatc accgggcgcg cccgtgacgg ggcggagtgc caccaccggc
     6601 tcggagctcc cggccatggc cggcggctcg tcctggacga ggcgcgcaat ctcgggcgcc
     6661 gcgacggcgg cgacgctgcg cgcgtcgccg cccgccgctg atccatgacc gagcgagcgg
     6721 ccgtcggcgc tgcggagctc ccaatcgaca cggaaggccg cctcgctccc ctcaggcggc
     6781 ccgccggcgg cgaggcggag ctcgaagctg cgcttgttgc ggccctgggt gctcgcgggg
     6841 atctcgcgct cgcgcagcgc cgcggcgatg ccctcggcga ggtcggacgg cgtcccgggc
     6901 agcggctcga cgcggacgcc ggcgctgtcg cgcggggcga gcagcgagga gtcgggccgg
     6961 tcatccgcga aaggatgcgg cagcggctgg catgccgcga ggagcaaggg cagaaatgcg
     7021 atcaagcgca gcatggctca gacgaagcgt ccgcgggaag aacgggccga cgaaccggcg
     7081 acgtcttcat ttatgggcag gcccgggcct tcatgctagg gcgacaggat gacgctccgg
     7141 agacatgccg gcgtcgcgtt tgcgggcgac ggtcgcgacg gcgatcgagc aaccggctca
     7201 gttcacttgc cgctcgtggc caagccaata gggcttgcgc agctcgcgct tcaggatctt
     7261 gcccgacggg ttgcgcggca gcgcgtcgat gaagtcgacg gagcgcggca ccttgtagcc
     7321 ggcgatccgc tcgcgcgcat agtcgatgag ctccttggcg ctcacggcga cgcccggctt
     7381 cttgacgacg atcgctttca cggcctcgcc ccattgctcg tcggggacgc cgatgacggc
     7441 gacgtcggcg atggcgggat ggccgaagag cgcgctctcg acctcggccg gatagatgtt
     7501 ctcgccgccg gagatgatca tgtccttcac ccggtcatag atgaagagat aaccctcctt
     7561 gtcgaggtag ccggcatcgc cggtgtggaa ccagccgtcg cggatcgcgc gcgacgtcgc
     7621 gtcggggagg ttccagtatc ccagcatcac ctggggcgtg cggcagacga cttcgccgac
     7681 ctcgccgggc ggcagcttct tgccgagagc gtcgacgatc ccgatctcga cgccgtctgc
     7741 gggcttgccg caggacttca tgcgctcgat ggcgtgctcg ccgtgatcct cgggcggcag
     7801 gtaagtgatg gcgcccgtgg tctcggtcag gccatagact tgcgccaagc cgcagccgaa
     7861 gaccgcgcgc gcgttgcgca gcagatcgag cggggcaggc gaggcgccgt agacgatgag
     7921 ctcgagcgag gagaaatcgg tcgcgcggca ttgcggcgcc tgcagcagga acagcatcat
     7981 cgccggaacg aagagcgtct tggtgatccg ccgctccgcg atcaggcgaa ggatcgtcag
     8041 cgcatcgaaa tcgcgggtca ggacattctc gacgccgcga tagaggccga tcaacgccca
     8101 gccgctgccg ccgatgtgga agagcggcat gcagacgagg ttgacgtcgc cgtcgcgcca
     8161 gacgccccac tgccggagcg cgcccggcaa gagggtgaga aggttgtcgt ggctgagctg
     8221 cgcgcccttg ggatggccgg tggtgccgct cgtatacatc tggatcgcgc agtcgagccc
     8281 ggcgagcggc cgcatcgggt cgctcgcgtt ctcgcgatcg cgccagcggg ggtagtcgat
     8341 ccagcccgga tgctggccgt cgagggccac gatctcgcgc acgctcgtga gctccgccgc
     8401 caccttgtcg atgacgtcgt aatagtcggg gccgacgaag agcagatccg cacgggcgtc
     8461 gttcacgaca taggcgatct cgggcggcgc caggcggaaa ttgacgggga ccagcacgac
     8521 gccggccttg gccgcgccga accacagctc gaagaattcg ggggcgttct tcgcgagcac
     8581 cgcgacgcgc gcctcgcgct cgatgccggc gcgtatgagg ccttgcgcca cctggctcgc
     8641 gcggcggtcg agcacgccgt agctgatccg ctcgccgtcg aaatagagag cggtctggtc
     8701 cggccgctcg cgggcgtggt gtcgcgttac atcggcgacg gtggaaatag cagatatcgt
     8761 cgatacggcg cgctcggcgg cgctcgcgga catggcggaa tcctccctcc caggagacgt
     8821 tttccgccat cttaacctgg aattcccgga cgggcacgcg ccgcgacggt gtcccggaga
     8881 gggggatgag cagaaggctt agccggttcg accctgagcc gccgacggcc gcggaccgca
     8941 gagcggacgc agcgcctcgc gctcggcgcg cgagagcgtc gtcagcggat agatcggctc
     9001 gcgcgtcgcc cggtcgaaaa aaggatgcgg ccaggtccgt ccggtgaggc gccatcccag
     9061 gatcttgcgc agcatccgcg cgatgagacg cgctttgaat ccggtcgagc cctctggcgc
     9121 gtcgccgacg gcgaattttc cgtggacggc gccgagcggc gggccgaaga cgtcatcggc
     9181 cttctggctc gcgctgtcga agacgcgcgc gacgatcccg acgaagggca tggccgggcc
     9241 gagcgtgttg ccgagcggcg tcttgcagca gctcgcgtac cagcgataga ggcccttggg
     9301 cgacaggcgc agaccctgga tgcggtcctt gccctgaacg aaggagagcg ccgctggagc
     9361 gacctggacg acgtcggtgc cgccatgggc atcgaagaga tcggtgcggc ccaggtggtg
     9421 aaggtaggct tggcagtcgt cgcaatagca gacggcacgg ttcacggcgt ccggcgccgc
     9481 gttcgcaacg acaccgcgga ccgctccgca ccggcactgc agcgctgcgt tctttcccat
     9541 ccggccctcg gcggcgattt tggaacaagg gtacggcgcg ggcaatcatg aaatgaatcg
     9601 gtggctaccc accgtcatcg atatggtcgg gcggcaggcc ggcttgatag cgctcgtaca
     9661 tcgcaaggta ggcccgctcg agatgacgcg tggatgatcg cgtgtcgaac aagggggcgc
     9721 tcaggcgcgt ctgcgcgagc ttttcccgca ggcgcgacag ctcggcgggg tgcgttgcca
     9781 ggtcgagcgc gcgctgctca taggcaccgc gcgtctcggt gatgagttcc ggcaggccga
     9841 ccgcgcggag gagggatgcc gcgaccctgc cggcgaaagt ctcgccgatc tgggtcagca
     9901 ccggcacgcc tgcccagagc gcgtcgctcg cggtcgtgtg cgcgttatag ggcagcgtgt
     9961 ccaggaacag gtcggcggcg cggtgacgcg ccagatgctc cggcagcgcc gcccgtttcg
    10021 cgaagacgag gcgatccgga tcgacgccgc tcatccgcgc ttcctgtcgc agattggcgg
    10081 ccgcgccggc attgtcctcg agcagccaca gcacgctgtc ttcccgctgc ttaagaagcc
    10141 gcatccagca cgcgaagacg tcggggaaga tcttgaagtt gttgttgaag cagcagaaga
    10201 cgaagcccgt gtccggcagc cccagctcgg cgcgcgcaaa ggatcggccc gagatcggcc
    10261 gcttcgcgtc gttgatctgg tagctgccgg gcacataggc gatcttctcg gcgtagtgcg
    10321 ccttgtgcga cgccgggacg acgatgcggt cggcgatcag gtagtcgata tagtccgcgc
    10381 ccatcgtcgc cgggaatccc agatagttga cctgaatggg cgcggggcgc cgcgcgaaga
    10441 cgccggtgcg ggcgtcctgc gtgaagccct tgaggtcgac caggatatcg atctcggcgc
    10501 ttctgacgcg ctcggcaatc gcgtcgtcgc tcaacgcccg ggcctcgatg aactggtcga
    10561 aggcctgctt caagcgcccg cgcatctcgg acgcgtcatc ctgtccgacg gatatcgccg
    10621 ttatgtcgaa acgcgatttg tcatggcact cgaagagccc ggcggcgaga taggcggtcg
    10681 cgtgctgatg aaaatcggcc gagacataag cgacgcgaat gcgctcgtgg cgatagatcg
    10741 gcccctgcca gatgcggggc gcaagcgcgg gatatttcgc cgccgaccag gatcgcgcgc
    10801 agtcgagctg atcctcgggc gaggcggaga ggttgtagaa ggcgaagggc gtggcattcg
    10861 ccttcttctg tctgacggcg gcgatcagat gctcgcgctc cgccgcgaca tcgcgccagt
    10921 cgcagagcag catcttgcag tgaagacgca tcccttcgat gcccggcaga tcgggcgcga
    10981 gcgccagcgc tttctcgtaa tccgcggtcg cttccgcata gcggccgagc atgcgcaatc
    11041 cgtccgcgcg gcagacccgc gcctcggcga agtccggctt ggcggcgagc gccttgtcat
    11101 aggccgcaaa ggcgtcctca tagcgcttca gctcgcggaa gacgttgccg cgcccgagcc
    11161 aggcttccgc caggtccggc ttgacggcga gcgcccggtc gtaagccgcg agagcttcgt
    11221 cgaagcgctt gagctcggcg agcagattgc cgcgaccgtt ccacccgccg gccaggccgg
    11281 gctttaactg cagggccttg tcgtagtcgg ccaaggcctc gccggagcgg ttgagatcgg
    11341 caagcacaaa agcgcggcca agccgtgcct cggccgcatc gggcgctaac gcgagcgcct
    11401 tgatgtaagc cgccagcgcc tcgccgcgaa gctgctgctc gcgcaggaca gtgccgcggc
    11461 cgagccacgc ctcgagcaga tcgggtttca gtctcagcgc gctatcgtag gcggcgagcg
    11521 cctcgtcatg gcgcttgagc agcgacagcg attttccctt gttgcacaag gcgccgtcgc
    11581 aattcgggtc gatcgagagg gccttgtcga aatcggcgat cgcgcggtcg tagtccttca
    11641 agtcattgcg cacggtcccg cgactgttcc aagcgtcgca ggcgttcgga tcgagagcga
    11701 gcgcccggtc gaactgctcc aaagccggcc gcggcttgcc cagcatcttg aggatgatgc
    11761 cgtagttata gaacgaagcg gcggatccct ggttgatctt cagggctctc gtgatgaaag
    11821 cctcggcttc cgcgaagcgt ccccgactca tcaggacggc cgtcagcaga ttcaaagccg
    11881 cgaaattccc gggctctttc gccagcatcg tcttgaagag acgctcggcg tcatcgaagc
    11941 gccgaagatt gagcgccgag atcgcgcttt ggaagatcgt ttgcgccgtc gtgaagtcgc
    12001 tcacctctca ccccttcggc attctcggcc gaaccccagc gtctattcag caaggcagat
    12061 cgccgacagc gcgcgcccgt aatcggtctc cttgcgcaag catgcgcggc gatacgagaa
    12121 gaagcggtcg ggctcggcgg cggtgtcgcc gcc
//
LOCUS       DASYNS010000079         3914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2361309, whole genome shotgun sequence.
ACCESSION   DASYNS010000079 DASYNS010000000
VERSION     DASYNS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2361309"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VGP50_02475"
     CDS             complement(<1..767)
                     /locus_tag="VGP50_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEV8026264.1"
                     /translation="MSDTPVPQTDPKAAYLAQRAAIDAAIARVLAAGRYILGPEVAAF
                     EQEFAAYVGVRHAIGVASGTDALVLSLRALGVAAEDYVATVPHTAVATVAAIELAGAR
                     PLLVDIDPATYTMDAAALARALAAPPGRIAAVIPVHLYGQPADPGILALARRHGARVI
                     EDCAQCHGALIDGRRAGSIGDVGAFSFYPTKNLGALGDGGIIVTDDDTLAERLRALRE
                     YGWRARYVSDIAGMNSRLDELQAAILRVKLAALDADNA"
     gene            complement(771..1766)
                     /locus_tag="VGP50_02480"
     CDS             complement(771..1766)
                     /locus_tag="VGP50_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV8026265.1"
                     /translation="MPADLTAAFAGARVMVTGGLGFIGSNLAIKLVALGADVLLVDNL
                     FPDYGGNLFNVAPVSDRVSVELADIRDVAAMRELLGGCRYLFNLAAQTSHLDSMTDPE
                     TDLAINCHAQLTLLEACRAVCPDIRIVHASTRQIYGRPRYLPVDEKHPLRPVDVNGVN
                     KMAGEAYHLLYHDVHGIAATVLRLTNTYGPRLRIRDARQTFLGVWLRAVVEGRPFEVW
                     NGAQRRDLTYVEDAVDAFLLAAATAETKGGAFNIGAERAVSLKELADMLVAANGGGSY
                     ETHEFPPERKRIDIGDYYADDALFRRATGWQTATPLEEGLRRSLDYVRDNLSAYV"
     gene            complement(2390..2752)
                     /locus_tag="VGP50_02485"
     CDS             complement(2390..2752)
                     /locus_tag="VGP50_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV8026266.1"
                     /translation="MTSLRLDKWLWFARFAKTRSLAAKLCAAGCVTVSGAPVSKAGHP
                     LRVGDVVTVRQGRMLRRVTVAALGERRGPPSEARLLYAEPEMPLSLRDLERMAWTPLI
                     EDEQSDAPAPPLERRMRS"
     gene            complement(2749..>3914)
                     /locus_tag="VGP50_02490"
     CDS             complement(2749..>3914)
                     /locus_tag="VGP50_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="disulfide oxidoreductase"
                     /protein_id="HEV8026267.1"
                     /translation="ARAIEDRLSDALHDRITQRFVDRRSAFLVKQLASEKDLLASVSH
                     TGDVKVEGHYVGRLDGFRFLPDEQAAGGETKTLLAAANRVLRGEIAVRARRLAAAPDS
                     EFALTPLGCVTWHGGTVGRLSRGDSALLPRVEPLPGDFLEGDLRDGVRRRLASFVRDA
                     LRHGLAPLFRARDATLAPAARGLAFQLTEALGSLPAGATTEQRAALVPADRRALARLG
                     VRFGTESVYIEALLKPRPAGLRALLWALWTGAAPPAVPAELAATRDAMISEEAYGAIG
                     YRVLGPRVLRIDRVERLAALARRLARQGPFGAAPELAMLAGCPVDALPSVLPALGYRA
                     VLAEDGSVTFHARPHRGGKRSRSRSERRRGRRGGTEKDMDSPFAKLRELRLAR"
BASE COUNT          685 a         1317 c         1349 g          563 t
ORIGIN      
        1 gcgttgtcgg catcgagcgc ggcgagcttg acgcggagga tggccgcctg cagctcgtcg
       61 agccgcgagt tcatgccggc gatgtcgctg acatagcgcg cgcgccagcc atattcgcgc
      121 agcgcccgca agcgctcggc gagcgtgtca tcgtcggtga cgatgattcc gccatcgccg
      181 agggcgccca gattcttcgt cggatagaag ctgaaggcgc cgacatcgcc gatacttccc
      241 gcgcggcggc cgtcgatcag cgcgccatgg cactgcgcgc agtcctcgat gacgcgcgcg
      301 ccgtgccggc gcgcgagcgc cagaatgccg ggatcggccg gctggccgta gagatggacg
      361 ggaatcaccg cggcgatgcg gcccggcggc gcggcgagcg cgcgtgcgag cgccgccgcg
      421 tccatggtat aggtcgcggg atcgatatcg acgaggagcg gccgcgcgcc ggcgagctcg
      481 atcgccgcca ccgtcgccac ggcggtatgc ggcaccgtcg cgacgtagtc ctccgccgcc
      541 acgccgagcg cacgcagcga cagcacgagc gcgtcggtgc cgctggcgac gccgatcgcg
      601 tgccggacgc cgacataagc cgcgaattcc tgctcgaacg cggcgacctc ggggccgaga
      661 atgtagcggc cggccgccag cacgcgcgcg atcgccgcgt cgatcgctgc gcgctgcgca
      721 agataggccg ccttgggatc cgtctgcggc accggcgtgt cgctcatgac tcagacatac
      781 gccgagagat tgtcgcggac atagtcgagt gagcgccgca atccctcttc cagcggcgtc
      841 gccgtctgcc agccggtggc gcggcggaac agcgcgtcgt cggcatagta gtcgccgatg
      901 tcgatgcgct tgcgttcggg cggaaactcg tgcgtctcgt agctgccgcc gccattggcc
      961 gcgaccagca tatcggcgag ctccttgagg ctcacggcgc gctccgcgcc gatgttgaag
     1021 gcgccgcctt tcgtctccgc tgtggcggcg gcgaggagaa acgcatcgac cgcgtcctcc
     1081 acataggtga ggtcacggcg ctgcgcgccg ttccacacct cgaaggggcg tccctcgacg
     1141 accgcgcgca gccagacgcc gaggaatgtc tgtcgcgcgt ccctgatcct gaggcgcggc
     1201 ccgtaggtgt tcgtgagacg cagcaccgtc gccgcgatgc catggacgtc gtgatagagg
     1261 agatgatagg cctcgccggc catcttgttg acgccgttga cgtcgacggg ccggagcgga
     1321 tgcttctcgt cgaccggaag gtagcgcggc cggccataga tctgtcgcgt gctggcatgg
     1381 acgatgcgaa tgtccgggca taccgcgcgg cacgcctcga gcagcgtgag ctgggcgtgg
     1441 cagttgatgg cgagatccgt ctcgggatcg gtcatcgaat cgagatggct cgtctgcgcc
     1501 gcgagattga agagataacg acagccgccg agcagctcgc gcatggcagc gacgtcgcgg
     1561 atatcggcaa gctcgacgct gactcggtcg ctgacggggg cgacgttgaa gagattgccg
     1621 ccgtagtcgg gaaagagatt gtcgaccagc agcacgtcgg cgccgagcgc caccagcttt
     1681 atggcgagat tggagccgat gaagccgagg ccgccggtga ccatcacccg cgcgcccgcg
     1741 aacgcggccg tcaggtcggc cggcatggca ggcgggcagc cgggttcagg gcgaacggca
     1801 tggccgccga tcctcgccga ggaacggggc aagcgcaagc cggcgccctc cggcgaaatc
     1861 gctggacgtg gcgaatgatt tggcacttcg gctcatcgag aaggcatcgc ccggcaacaa
     1921 tttggcaccg ccaaattctg ttaccgggtc agtcaaatgg taacaggcgg aaaacaaata
     1981 aactctcgca tccggtgctc gccccgcaaa taagacaagg ccgacgcggc tgttacgttc
     2041 tcctacgcaa atgtttcgaa gaattacgga agaagcgcct ggcaactcct gttgactgaa
     2101 cgagggcaac agccgacagg atggcgaaat gacagcgggt cgtgccgatg agatccgata
     2161 tttacgagag aaagctaaac aatttcgtga acttgccagg acctatagga acgacatttc
     2221 agccaagctg ctcgagatcg ccagtgatct cgagcgtcgt gccgaggtct tggagaaaca
     2281 ttagctaatt agctaaatta gactaattag ctaaaaagaa gcacggcgtc gtctggccga
     2341 acgcgtcccc gcccaaaagg aaggcactct ccaatccctt ggacgtggtt catgatcgca
     2401 tccgcctctc caaaggaggc gcgggggcgt cgctttgctc gtcctcgatg agcggtgtcc
     2461 aggccattcg ctcgaggtcg cgcagcgata agggcatctc cggctccgca tagagcagac
     2521 gggcttcgga gggcgggccg cggcgctcgc ccagcgccgc cacggtgaca cgccgaagca
     2581 tgcgcccctg ccgcaccgtg acgacgtccc cgacgcgcaa gggatgccct gctttcgaga
     2641 ccggggcgcc gctaacggtg acacagcccg cggcgcagag cttcgccgcc agcgagcgtg
     2701 tcttggcgaa gcgcgcgaac cacagccact tgtcgagacg caggctcgtc atcgcgcgag
     2761 ccgcaactcc cgaagcttgg caaacgggct gtccatgtcc ttctcggtgc cgccgcgccg
     2821 gccgcgccgc cgctccgacc tgctgcggga ccgcttcccg ccacggtgcg gccgcgcatg
     2881 gaaggtcacg cttccgtcct cggcaaggac ggcgcgatat ccgagcgcag gcagcaccga
     2941 agggagcgca tcgaccgggc agccggcaag catggccagt tccggcgcgg cgccgaacgg
     3001 gccctgccgg gcgaggcgcc gtgccaaggc cgcgagccgc tcgacgcgat cgatgcgcag
     3061 cacgcgcggc ccgagcacgc gatagccgat ggccccatag gcttcttccg aaatcatggc
     3121 atcccgcgtc gcggcgagct cggccggcac cgctggcggc gcagcgccgg tccagagtgc
     3181 ccagagcagc gcgcgtaagc ccgccggccg cggtttcagc agggcctcga tatagacgga
     3241 ctcggtgccg aatcggacgc ccagccgggc cagcgcccgg cggtcggccg gcaccagcgc
     3301 cgcgcgctgc tcggtcgttg cgcccgccgg caggctgccc agggcctcgg tgagttgaaa
     3361 tgcgagaccc cgcgccgccg gcgccagcgt cgcgtcgcgc gcccggaaca agggcgcgag
     3421 gccatggcgc aacgcgtcgc gcacgaagga cgcgagacgg cggcggacgc cgtcgcgcag
     3481 atcgccctcg agaaagtcgc ccggcagcgg ctcgacgcgc ggcagcaacg cgctatcgcc
     3541 ccgcgacaac cggccgaccg tcccgccatg ccaggtgacg cagcccaggg gcgtcagcgc
     3601 gaactcgcta tcgggcgccg ccgctaaccg ccgcgcgcgc accgcgatct cgccgcgcag
     3661 cacgcgattg gcggccgcca gcagagtctt ggtctcgccg ccggcggctt gttcgtcggg
     3721 caggaagcgg aagccgtcga gccggccgac atagtggccc tcgaccttca cgtcgcccgt
     3781 atgactgacc gaggccagca gatccttctc cgaggcgagt tgcttcacca ggaaagccga
     3841 gcggcggtcg acgaagcgct gggtgatgcg atcgtgcagc gcgtcggaaa gccggtcctc
     3901 gatggcgcgc gccc
//
LOCUS       DASYNS010000080         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1892191, whole genome shotgun sequence.
ACCESSION   DASYNS010000080 DASYNS010000000
VERSION     DASYNS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1892191"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VGP50_02495"
     CDS             <1..642
                     /locus_tag="VGP50_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV8026268.1"
                     /translation="SYVRSIFERYAASFDSHLDRLGYRAPQLLTAALARVIGEPQRSL
                     DILDAGCGTGLCGPLLRPFARRLEGVDLAEAMLAKARERDVYDALAAAELTAFIAARK
                     DTYDVILSADTLVYFGDLAAVVKAAATALHSGGILAFSLERCASDGAGPGYRLEEHGR
                     YSHAESYVRRVLAETGFAPIAVDAATPRNEGGAPVAGLVVVARKSASEAHSNP"
     gene            complement(627..>1968)
                     /locus_tag="VGP50_02500"
     CDS             complement(627..>1968)
                     /locus_tag="VGP50_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8026269.1"
                     /translation="VETAQREGQANEKLTAERERLKGLETRWQEEKALVDRILEMRAK
                     LRGTSGTVEGTSSALEKAAAVAKAAEPGPAAAAAPLTGLDRERLLSKLHDAQAQLAEL
                     QGETPLILPSVDAQAVASVVQDWTGIPVGRMVKNEIETVLKLADTLSQRVIGQRHGLE
                     MIVKRIQTSRAGLDNPYKPIGVFMLCGPSGVGKTETALALAEALYGGEQNIITINVSE
                     FQEAHTVSTLKGAPPGYVGYGEGGILTEAVRRKPYSVVLLDEVEKAHPDVHEIFFQVF
                     DKGQMEDGEGRLIDFKNTLILLTTNVGTDLIANMCKDPELMPEPEGLAKALREPLLKV
                     FPPALLGRIVTIPYYPLSDAVMAAIIRLQLGRIEKRVKATHKIPFTYDDDVVTLIAER
                     CTEVESGGRMIDAILTNTVLPTISREFLERTMEGRPVDRVHISVADGDFRYGFE"
BASE COUNT          289 a          681 c          643 g          355 t
ORIGIN      
        1 agctatgtgc gctcgatctt cgagcgctac gcggcgagct tcgattcgca tctcgaccgg
       61 ctcggctatc gggcgccgca gctgctgacc gccgccctcg cccgggtcat cggcgagcct
      121 caacgctcgc tcgacattct cgacgccggc tgcggcaccg gcctttgcgg cccgctgctg
      181 cgccctttcg cccgccgcct cgaaggcgtc gacctcgccg aagccatgct ggccaaggca
      241 cgcgagcgcg acgtctacga cgcgctcgcc gcggccgagc tcacggcctt catcgcggcg
      301 cgaaaggaca cttacgacgt cattctctcc gccgatacgc tcgtttattt cggcgacctg
      361 gcggccgtcg tgaaagcggc cgctacggcg ttgcacagcg gcggcatcct cgccttctct
      421 ctcgagcgtt gcgccagcga cggtgccgga cccggctatc gcctcgagga gcacggacgt
      481 tacagccatg ccgagagcta tgtccgccgc gtgctcgccg agaccggctt cgcgccgatc
      541 gcggtcgacg ccgccacccc gcgcaacgag ggcggcgccc ccgttgccgg gctggtggtg
      601 gtcgcacgaa aatcggcgag cgaagctcac tcgaacccgt aacggaaatc gccgtccgcc
      661 acactgatat ggacgcggtc gaccggccgc ccctccatcg tccgttccag gaactcgcgg
      721 ctgatggtcg gcagcacggt gttggtgagg atcgcatcga tcatgcggcc gccgctttcg
      781 acttcggtgc agcgctccgc gatcagcgtc acgacgtcgt cgtcgtaggt gaacggaatc
      841 ttgtgcgtcg ccttgacgcg cttctcgatc cgaccgagct gcaggcggat gatcgccgcc
      901 atcaccgcgt cgctcagcgg gtagtacggg atcgtcacga tgcggccgag caacgccggc
      961 gggaagacct tgagcaaagg ttcgcgcagc gccttggcca gaccctcggg ctcaggcatc
     1021 agctccggat ccttgcacat gttggcgatg agatcggtgc cgacattcgt cgtcagcagg
     1081 atgagcgtgt tcttgaagtc gatgaggcgc ccctcgccat cctccatctg gcccttgtca
     1141 aagacctgga agaagatttc gtggacgtcg ggatgcgcct tctcgacctc atccagaagc
     1201 acgacgctgt agggcttgcg ccggaccgcc tcggtgagga tgccgccctc gccatagccg
     1261 acatagcccg gaggcgcgcc tttaagcgtc gagacggtat gcgcctcctg aaactcgctc
     1321 acattgatgg tgatgatgtt ctgctcgccg ccgtatagcg cctcggcaag cgccagcgcg
     1381 gtctcggtct taccgacgcc cgacgggccg cacagcatga agacgccgat cggcttgtag
     1441 gggttgtcga ggccggcgcg cgacgtctgg atgcgcttca cgatcatctc gaggccgtgg
     1501 cgctggccga tgacgcgctg gctcagcgta tcggcgagct tcagcaccgt ctcgatctcg
     1561 ttcttcacca tccgtccgac ggggatgccg gtccagtcct ggaccaccga ggcgacggcc
     1621 tgggcatcga cgctcggcag gatgagcggt gtctcgccct ggagctcggc gagctgcgcc
     1681 tgcgcgtcgt ggagcttgga gagcaggcgc tcgcgatcaa ggccggtgag cggggccgct
     1741 gccgcggcgg gaccgggctc ggcggccttg gcgacggcag ccgccttctc gagggcgctg
     1801 ctcgtgccct cgacggtgcc ggaggtgccg cgcagcttgg cgcgcatctc gaggatgcgg
     1861 tcgaccagcg ctttctcctc ctgccagcgt gtttcgagac ccttgagccg ctcacgctcg
     1921 gcagtgagct tctcattggc ctgcccctcg cgctgcgccg tttccacg
//
LOCUS       DASYNS010000081         7511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1581751, whole genome shotgun sequence.
ACCESSION   DASYNS010000081 DASYNS010000000
VERSION     DASYNS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581751"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VGP50_02505"
     CDS             <1..792
                     /locus_tag="VGP50_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026270.1"
                     /translation="YARRGIKLNLVTNLTYFNDKLWQRIGHNRIGIITMSADGCSPEV
                     YNSIRVGADWDTVAANMRLLAEIRRQGRVESIVWNYTVLRRNVCDVAKAIRFAGELGF
                     DRFRLIAQFGELRRTEGNIFESFDLDALDALHAELESVDAFDNPMVWMFEIGMRGRRY
                     RTPEFALAFAQHVYERQGYGSDGAEPSLYNLAKALKIVGRLMHDIGSGRIPRPQRLPD
                     GALRFLERLANHAERHYQPDAGAAPEAAEARAAAAGTRALLARLG"
     gene            797..2581
                     /locus_tag="VGP50_02510"
     CDS             797..2581
                     /locus_tag="VGP50_02510"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEV8026271.1"
                     /translation="MATPRSPGDEPMFRGIAPLRGFGAEERDAEGVVHVAVRSFCRVQ
                     RLGSGRYLQCHLDFPAQRGELVASVGGRIVDRAELTTGWQRSAFELKDVPVPAELGLA
                     FRDGAGKNVTGVRIRSLGVSDAWGQGQRKRLKCHVPFRDIAVFADFSVYPCCARQWLK
                     DNQVAGNTGEQLLEEIWNGPQYQRVRRLFLEGRYDEVCRAEVCPYLTGEMKAGEPTPE
                     IIAAVNEGHTAVAHGPQLLHHDIDRGCNLECVMCRNEKILPREENVDRGLRDIDDALA
                     LGSLRWMTFSGAGEIFAMKKVVQLLESQKLSARGVKLGVNTNLTHFTEKLWRRIGHNK
                     FYTVTLSADGCSPEVYNAIRIGADWLEVAGNMRFAARLRREGQIECLVWNYTVMKQNI
                     GDVGKAVALSRELGVDWIRLIAQLGELNRTDGNMFEDYDRTALDALHAELDRAGALDD
                     PRVCISEIAMRDRRYRTPEFRLELAEHLYQRRNFGSDKSIPLVDRDWRKCLNLVTGVL
                     SDVATGDVAALQHLTDDHLRFLERFAAYTQERCGVGPASGGEDSLAAEARRVAGRSRA
                     LVATRRGGSQGIVQRLLARTTAWCRSVR"
     gene            complement(2553..3242)
                     /locus_tag="VGP50_02515"
     CDS             complement(2553..3242)
                     /locus_tag="VGP50_02515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HEV8026272.1"
                     /translation="MNPHFDLEPAFLAIYEQCRAETMTSIERLYALYKAVEHVVDAGI
                     SGDFVECGVWHGGSVMMMALSAAQFGVSDRTIWLYDTFSGMPPPAAIDVQSLTGISAS
                     ALLDANERSETNPFWAITTRGRVEANLRRTGYPAERFRIVEGDVLQTLPGEAPREIAI
                     LRLDTDWYRSTRHELVSLYPRLTAGGALIIDDYGYWEGARRATDEFFAREKRRPMLHR
                     IDFTGRICTKP"
     gene            complement(3423..4049)
                     /locus_tag="VGP50_02520"
     CDS             complement(3423..4049)
                     /locus_tag="VGP50_02520"
                     /inference="COORDINATES: protein motif:HMM:NF016850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HEV8026273.1"
                     /translation="MTSDIPYPLLMSIQKATMAHRYRGVAMLKNPFDLALYPLLLAQA
                     RPRTLIEIGSHQGGSALWFADTAAALGFPMHVHSIDLAEVDGVHHPEVTFLRGDARDL
                     RTVLGDDRVAALPRPLLVVEDSDHRFGTCLAVLAFFDRWLRPGEYIVVEDGILSDMQV
                     AELYDGGPLRAIEQFLAATGGRYVVDREFCDYFGRNVTWNVDGYLRRV"
     gene            complement(4046..4888)
                     /locus_tag="VGP50_02525"
     CDS             complement(4046..4888)
                     /locus_tag="VGP50_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026274.1"
                     /translation="MFFKRRPRIAELSGMEWRQRAVPAACAGVQTMLLPDELRLLDFL
                     ADTYYEGRGVIVDAGCFVGGSTVALAAALRRNLARRRRREQHLIHSYDIFAIEEWTRG
                     IFFPETAQAGDSFRPIFDRNIADFADLVAVHPGNITAAPWIGAPIEILFVDVAKHWTV
                     CDWLSANFFRHLIPGRSIVIQQDYLYHHWNAWIHVTMEYYRDHFEMLFDTTYNSVVFL
                     HTKKFGRRAIQPKTVASLSDAEKATLMDRAAARFSGEQRHMLVAAKEHFLGMLAAERE
                     PGFR"
     gene            complement(4901..5548)
                     /locus_tag="VGP50_02530"
     CDS             complement(4901..5548)
                     /locus_tag="VGP50_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8026275.1"
                     /translation="MRVKPAFARVGPVHFQPLEWELEPVARYLGGALLNAGCGDRDLG
                     SYFAGIGVTRLTNYDIRSTLPDAVLGPLEKMPFAAGQFDAALCNAVLEHVASAEAVMA
                     ELSRVLRPGGHLVLAVPFLQPFHECPIDFRRYTGDGLAALGEAAGFDVVAVLPVHSMA
                     QTLGWIAWEFALEKKSMLLRWTVWPLVFAATRLWCRTDRGLRRNANTYQVVLVRP"
     gene            complement(5545..6648)
                     /locus_tag="VGP50_02535"
     CDS             complement(5545..6648)
                     /locus_tag="VGP50_02535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV8026276.1"
                     /translation="MSGGLMIHVISGLHGGGAERLLTAIALGSLAAAQKIIVVSLLPG
                     GEFRPCLEAAGIAVCDLGMRRRVPSLAGLLRFVALLRAHQPTVVQGWMYHGNLVACIG
                     LMLSGRWSRTFLYWGLYCTAMTSGERHWRLRLVLWLSARLSGLTAGIFYNAVSARDYH
                     RSIGFHEPHSLILCNAVDTSLFGIHPELRSQVRRELRIAEDAMVVVSAARVDAMKDWR
                     TLMAAVRPLANLVTLAVGKGTESLPDQSGFIRLGWRCDLHRIFNAADIFVLASAFGEG
                     RSLAMTEAMACGLPVVTTDVGDHALLSAGAGVAVPPRDPGALTAEIRRLAADPALRRR
                     WGEAARARVVEDHSLARSYESFRRGHAGAMANE"
     gene            6818..>7511
                     /locus_tag="VGP50_02540"
     CDS             6818..>7511
                     /locus_tag="VGP50_02540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8026277.1"
                     /translation="MPDSRSLPETSNGLPAGDYVSPGLEIVRPDHCFPHMAAGDKLSH
                     PWKYLRREVPHNWYSDKRKPLMGFMDRDEAVLLYNIAREFRGRPALEIGCWFGWSTCH
                     LALGGVKLDVVDPILSDPLHRDSVEHALRCCGVRDAVTLHGAASPAAIAALAAKDRTV
                     WELFVVDGDHEPPMPERDVDACIAHAAEDAAFVLHDLASPEVAAAFRLLEARGFNVMV
                     YQTMQIMGIAWRG"
BASE COUNT         1347 a         2527 c         2436 g         1201 t
ORIGIN      
        1 tacgcgcgcc gcggcatcaa gctcaatctc gtgaccaacc tcacctactt caacgacaag
       61 ctctggcagc ggatcggcca caaccgcatc ggcatcatca ccatgtcagc cgatggctgc
      121 tcgcccgagg tctacaacag catccgcgtc ggcgccgact gggataccgt cgccgccaat
      181 atgcgcctgc tggcggagat caggcgtcaa ggccgtgtcg agtcgatcgt ctggaactat
      241 accgtgctgc gccggaacgt ctgcgacgtc gccaaggcca tccgcttcgc cggcgagctc
      301 ggcttcgacc ggttcaggct catcgcgcaa ttcggcgagc tcaggcggac cgaaggcaac
      361 atcttcgaga gcttcgatct cgacgcgctc gatgccctcc atgccgagct cgaaagcgtc
      421 gacgccttcg acaatcccat ggtctggatg ttcgagatcg gcatgcgcgg ccggcgctat
      481 cggacgccgg agttcgcctt ggccttcgcc cagcacgtct atgagcggca gggctatggc
      541 tcggacggcg cggagccgtc cctctacaat ctggcgaagg ccctgaagat cgtcgggcga
      601 ctcatgcacg acatcggctc cggcagaatc ccgcgcccgc agcgcctgcc cgatggcgcc
      661 ctccgctttc tcgagcgcct ggcgaaccat gccgagcggc attatcagcc ggacgcgggc
      721 gcggcgccgg aagctgcgga agcccgcgcg gcggcggccg gcacgcgcgc gcttctggcc
      781 cggctcggct gagcccatgg cgacgccgcg gtcccccggc gacgaaccga tgttccgcgg
      841 gattgcgccg ctgcgcggct tcggcgcgga agagcgcgac gccgagggcg tcgtccatgt
      901 ggcggtgcgc agcttctgcc gggtgcagcg gctgggcagc ggacgctacc tgcaatgcca
      961 tctcgatttc ccggcgcaac gcggcgagct cgtcgcctct gtcggcggcc gcatcgtcga
     1021 ccgcgccgag ctgacaacgg gctggcagcg ctcggccttc gagctcaagg acgtgccggt
     1081 tccggccgag ctggggctgg cgtttcgcga tggcgcaggc aagaacgtga cgggcgtccg
     1141 catccgctcc ctcggcgtca gcgatgcctg gggccagggc cagcgcaagc ggctcaaatg
     1201 ccacgtgccg ttccgcgaca tagcggtatt cgcggacttc agcgtctatc cctgctgcgc
     1261 ccggcaatgg ctcaaggaca accaggtcgc cggcaatacc ggcgagcagc ttctcgagga
     1321 gatctggaac ggtccgcaat atcaacgcgt gcgccggctg tttctcgagg gccgctacga
     1381 cgaggtctgt cgggcagagg tgtgcccgta tctcactggc gagatgaagg ccggcgagcc
     1441 gacgcccgaa atcatcgccg cggtcaacga gggccacacc gccgtcgccc atgggccaca
     1501 gctcctgcat cacgatatcg accgcggctg caacctcgaa tgcgtgatgt gtcgcaacga
     1561 gaagatcttg ccgcgggagg agaacgtcga ccgcggactg cgcgacatcg acgacgcgct
     1621 cgcgctcggc tcgctgcgct ggatgacctt ctcgggcgcc ggcgagatct tcgccatgaa
     1681 aaaggtcgtg cagctgcttg aatcgcagaa gctttcggcc cgtggcgtaa aactgggcgt
     1741 caacaccaac ttgacccatt tcaccgagaa gctttggcgc cgcatcggac acaacaaatt
     1801 ctacaccgtc acgctatctg ccgacggctg ctcgcccgag gtctacaacg ccatccgcat
     1861 cggggccgat tggctggagg tcgccggcaa catgcgcttt gccgcgcgct tgcggcgtga
     1921 aggccagatc gagtgcctcg tctggaatta cacggtgatg aagcagaaca tcggcgatgt
     1981 cggcaaggcg gtcgcgctca gccgagagct cggggtcgac tggatccggc tgatcgcgca
     2041 gctcggcgag ctcaaccgta ccgacggcaa catgttcgag gattatgacc gcacggcgct
     2101 cgacgcgctc cacgccgagc tggaccgcgc gggcgccctc gacgatccgc gcgtctgcat
     2161 ctccgagatc gccatgcgcg atcggcgcta tcgcacgccg gaatttcgcc tcgagctggc
     2221 cgagcacctc tatcagcggc gcaattttgg ctcggacaag agcatcccgc tcgtcgaccg
     2281 cgactggcgc aaatgcctca acctcgtcac cggcgtctta tccgacgtcg ctactggcga
     2341 cgtcgccgcg ctgcagcacc tcaccgacga ccatctgcgt tttctcgagc gctttgccgc
     2401 gtatacgcag gagcgctgtg gcgtgggacc cgcgtcgggc ggcgaggact cgctcgccgc
     2461 cgaagcacgt cgcgtcgccg ggcgaagccg cgcgctcgtg gccacccggc gcggcggcag
     2521 ccagggcatc gtccaacgcc tcctggcccg gactacggct tggtgcagat ccgtccggtg
     2581 aaatcgatgc ggtgcagcat cggccgccgc ttctcgcgcg cgaagaattc gtcggtcgct
     2641 ctccgtgcgc cctcccagta gccgtagtcg tcgatgatga gggcgccgcc ggcggtcagg
     2701 cggggataga ggctgacgag ctcgtgccgc gtgctccgat accagtcggt gtcgaggcga
     2761 aggatggcga tctcccgagg agcctcgccc ggcagcgtct gcagcacgtc gccctcgacg
     2821 atgcggaatc gctccgccgg atagccggtg cggcgcaaat tggcctcgac gcggccgcgc
     2881 gtggtgatcg cccagaacgg gttcgtctcg ctgcgctcgt tggcgtcgag gagcgcgctc
     2941 gcgctgatgc cggtaagcga ctggacatcg atcgcggcgg gcggcggcat gccgctgaag
     3001 gtgtcgtaga gccagatcgt ccggtcgctg acgccaaact gcgcggcgct cagtgccatc
     3061 atcatcaccg atccgccatg ccacactccg cactcgacga aatcccctga aatgccggcg
     3121 tcgacgacgt gctcgaccgc cttgtagagc gcgtagaggc gctcgatcga ggtcatcgtc
     3181 tcggcgcggc actgctcgta gatcgccagg aacgccggct cgagatcgaa atggggattc
     3241 atggcgcggc tcctctcaca cccagggcaa tcgcatatgc tgccgagacg ccgatcggaa
     3301 agatcgacca cggagacacg gaggacacgg agaagcgaag actggcgcgc gtcgcgcgcc
     3361 gagcccctct tctccgtgtt ctccgtgtct ccgtggtgaa acccatatgc gatagccctc
     3421 tctcacacgc gccggaggta gccgtcgacg ttccaggtga cgttgcggcc gaaatagtcg
     3481 caaaattccc ggtcgacgac gtagcgcccg ccagtcgccg cgaggaactg ctcgatggcg
     3541 cgaagcgggc cgccgtcata gagctcggcg acctgcatgt ccgagaggat gccgtcctcg
     3601 acgacgatgt actccccggg cctgagccag cggtcgaaga acgccagaac cgcaaggcat
     3661 gtgccgaacc gatggtcgga atcctcgacc accagcagcg gccgcggcaa ggcggcgacg
     3721 cggtcgtcgc cgagaaccgt tcggaggtcc ctggcgtcgc cgcgcaggaa ggtcacctcc
     3781 ggatggtgca ccccgtcgac ttcagcgaga tcgatcgaat ggacgtgcat ggggaaaccc
     3841 agtgccgccg ccgtgtcggc gaaccacagt gcgctgccgc cctgatgcga gccgatttcg
     3901 atcagcgtgc gcggccgcgc ttgcgccagc agcaagggat agagggcgag atcgaaaggg
     3961 ttcttgagca tcgccacgcc gcgatagcga tgcgccatgg tcgccttctg gatcgacatc
     4021 agcagcggat agggaatatc cgacgtcatc tgaagcccgg ctcgcgctcg gccgccagca
     4081 tgccgaggaa gtgctccttg gccgcgacga gcatgtgccg ctgctcgccg gagaaccgcg
     4141 cggcggcacg gtccataagg gtagccttct cggcatcgct caacgatgcc acggtcttgg
     4201 gctggatggc gcgccggccg aacttcttgg tgtgaaggaa gacgacggaa ttgtatgtcg
     4261 tatcgaacag catctcgaaa tggtcgcggt aatactccat cgtcacgtgg atccaggcgt
     4321 tccaatggtg atagagatag tcctgctgaa tgacgatcga ccgtcctggg atgagatggc
     4381 gaaagaaatt cgcgctgagc cagtcgcaga ccgtccagtg cttggcgacg tcgacgaaaa
     4441 gaatctcgat cggcgcgccg atccacgggg ccgccgtgat gttgccggga tggacggcga
     4501 cgagatcggc aaagtcggcg atgttgcggt cgaagatggg gcggaagctg tcgccggctt
     4561 gcgcggtctc ggggaaaaaa atgccgcgcg tccactcctc gatcgcgaag atgtcgtagg
     4621 aatggatgag gtgctgttcc cgccgccggc gccgcgccag gttgcgccgc aaagccgcgg
     4681 cgagtgccac ggtcgagccg ccgacgaaac agccggcatc gacgatgacg ccgcgccctt
     4741 cgtaatacgt gtcggcgagg aaatcgagca gccgcagctc gtccgggagc aacatcgtct
     4801 gcacgcccgc gcaagccgca gggaccgcgc gctgccgcca ctccattccg ctcagctcgg
     4861 cgatccgcgg acgccgcttg aaaaacatgc cgcttcctcg tcagggccgc accaggacga
     4921 cctggtaggt attggcattg cgccgcaagc cgcgatcggt gcggcaccag agccgggtcg
     4981 cggcgaagac gagcggccat accgtccagc gcagcaacat gctttttttc tccagcgcga
     5041 attcccaggc gatccagccc agcgtctgcg ccatcgaatg caccggaagc accgccacga
     5101 catcgaagcc ggcggcctcg cccaacgccg ccagcccgtc accggtatag cgccggaagt
     5161 cgatgggaca ttcatggaag ggttgcaaga acggtacggc gaggacgaga tgcccgccgg
     5221 gccgcagcac gcgcgacagt tccgccatca ccgcctcggc cgaggcgacg tgctcgagca
     5281 ctgcgttgca cagggctgcg tcgaactgac cggcggcgaa gggcattttc tccagcggtc
     5341 ccagcacggc gtccggcaac gtcgaccgga tgtcgtagtt cgtcagtcgc gtgacgccga
     5401 tcccggcgaa atagctcccg agatcgcggt cgccgcaacc ggcattgagc agcgcgccgc
     5461 cgagatagcg cgcgaccggc tccaattccc attccagggg ttgaaaatgc accggcccga
     5521 cgcgggcaaa cgccggcttg accctcattc attcgccatc gcgccggcat ggccgcgcct
     5581 gaagctctca tagctgcgcg cgagcgaatg atcttcgacc acccgggcgc gcgccgcctc
     5641 gccccagcgt cggcgcagcg ccggatcggc ggcaaggcga cggatctccg cggtgagtgc
     5701 gccgggatcg cgcggcggca cggcaacccc ggcgccggcc gagagcagcg catggtcgcc
     5761 gacatcggtg gtgacgaccg gcaatccgca ggccatcgcc tcggtcatcg ccaacgaccg
     5821 cccctcgccg aaggccgagg cgaggacgaa gatgtcggcc gcgttgaaga tacgatgcag
     5881 atcgcagcgc cagcccagac ggatgaagcc ggactgatcg ggcagtgatt ccgttccctt
     5941 gccgacggcg agcgtcacca agttcgcgag cggccgcacg gcggccatca gcgtgcgcca
     6001 gtccttcatc gcgtcgacgc gtgccgccga cacgacgacc atcgcgtcct ccgcgatccg
     6061 caactcgcgg cgcacttgcg agcgcagctc tggatgaatc ccgaagaggc tggtgtcgac
     6121 ggcgttgcag aggatgagcg aatgcggctc gtgaaacccg atcgagcgat gatagtcgcg
     6181 cgcgctgaca gcgttgtaga agatgccggc cgtgagaccg gaaaggcgcg cgctgagcca
     6241 gagcacgagg cgaagcctcc agtggcgctc accggaagtc atcgccgtgc aatagagccc
     6301 ccaatagaga aatgtccgcg accaccgccc cgaaagcatc agcccgatgc aggccaccag
     6361 attcccatga tacatccatc cctgcaccac ggtcggctgg tgggcgcgca acagcgcgac
     6421 aaagcgcagc aggccggcga gcgaaggaac acgccgtcgc atgccgagat cgcacacggc
     6481 aatgccggcc gcttccagac atggacggaa ctcgccaccg gggagaaggc tgaccacgat
     6541 aattttctgc gccgccgcga gactcccaag cgcgatcgcg gtcaacagcc gctcggctcc
     6601 gccgccatgc agtccgctga tgacatggat catcagacca cccgacatcc gttgctcgtt
     6661 cggcatcgac atgtgagcgg ggcggaatct gccgagcccc gcgcgttgct ccgtttcaat
     6721 atgaggggat gacaaccata tcacgttctc agggttggcc ttgtcattga tgttgcacac
     6781 gaccgtacga tcgcgcagcg ctgagccgag gtatcgcgtg cctgattcgc gctccttgcc
     6841 cgagacgtcc aatggtctgc ccgccgggga ttacgtctcg cccggcctcg agatcgtccg
     6901 tcccgaccat tgcttcccgc acatggccgc cggcgacaag ctctcgcatc cctggaaata
     6961 tcttcggcgc gaagtgccgc ataactggta ctccgacaag cgcaagccgc tcatgggttt
     7021 catggatcgc gatgaggcgg tgctcctcta caacatcgcc agggagtttc gcggccgccc
     7081 tgcgctcgag atcggctgct ggttcggctg gtcgacctgc catctcgcac tcggcggcgt
     7141 aaagctcgac gtcgtcgatc cgatcctttc cgatccgctg caccgcgaca gcgtcgagca
     7201 cgcgctccgt tgctgcggcg tgcgcgacgc cgtgacgctc catggcgctg cgagccccgc
     7261 cgccatcgcc gcgctcgcgg ccaaagaccg gacggtctgg gaattgttcg tcgtcgacgg
     7321 cgatcacgag ccgcccatgc cggaacgcga tgtcgatgct tgcatcgcgc atgccgctga
     7381 agatgcggcc ttcgtcctcc acgatctggc atcgcccgag gtcgccgccg cgtttcggct
     7441 gctcgaggca cgcggtttca atgtcatggt ctaccagacc atgcagatca tgggcatcgc
     7501 ctggcgcggc c
//
LOCUS       DASYNS010000082         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_488917, whole genome shotgun sequence.
ACCESSION   DASYNS010000082 DASYNS010000000
VERSION     DASYNS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488917"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1878)
                     /locus_tag="VGP50_02545"
     CDS             complement(139..1878)
                     /locus_tag="VGP50_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEV8026278.1"
                     /translation="MLSAGVVSRYVFRSPLTWSDELASILFLWLAMLGSVIALRRGEH
                     MRLTAIVNRLPQRWRGLADTVAALVVMTFMLLIMRPSLDYVAEEWAILTPGLEIHNSF
                     RAAAIAVGALLMMANALLRLAAYTGLAQLVVALLVTAGVAGALYLAQPALLALGSYNL
                     AVFFVGLVAAAIVIGVPIAFTFGIATLAYLGLMTHVPLIVVMGRMDEGMSHLILLAVP
                     LFIFLGVLIEMTGLARAMIDFLAALLGGVRGGLSYVLLAAMYLVSGISGSKAADMAAV
                     APALFPAMKQRGAAPGELVAQLAAAGAMSETIPPSIVLLTIGSVTGVSIAALFNGGLV
                     PALVLALALVVLVYFRSRGDDPGGEARPSPRQIARRFVVALPALVLPFLIRAVVVEGI
                     ATATEVSTIGIAYAVLAGLLIYRQFAWRRLYPMLVDTAALSGAILLIIGTATAMAWAL
                     TQSGFSGKLVAAMATVPGGKAGFLAVSILAFAVLGSVLEGIPAIVLFGPLLFPVSRAL
                     GIHDVHYAMVAILAMGIGLFAPPFGVGFYAACAIGRVSPDAAMPRIWPYLGALLLALV
                     VVAAVPWLSTGFL"
     gene            complement(2091..>2358)
                     /locus_tag="VGP50_02550"
     CDS             complement(2091..>2358)
                     /locus_tag="VGP50_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEV8026279.1"
                     /translation="PAWEALPKDLQEIVTHHWNAAALAQRDDIRLLNDTLQGDLEKKG
                     LIFNAPDTEAFRAVLKEAGFYREWREKYGADAWAVLEKYAGPLA"
BASE COUNT          462 a          766 c          852 g          278 t
ORIGIN      
        1 ttggcgacgg tggcggcgcg ctagagagcg catgtcgctc tgacggagcg ccgcctcggc
       61 gagcgtgccg ccaccgtcgc caagcgccct tcgagctgct ctgaccgcga cgaccgccat
      121 gacctggcgg actcctcgct aaaggaaccc cgtcgacagc cacggcacgg cggcgacgac
      181 gacgagcgcg agcagcagcg cgccgagata gggccagatg cgcggcatgg cggcgtcggg
      241 cgagacgcgg ccgatggcac aagcggcata gaagccgaca ccgaagggcg gcgcgaagag
      301 gccgatgccc atggcgagga tcgccaccat ggcgtaatgg acgtcgtgga tgccgagcgc
      361 gcgcgacacc gggaagagca gcggtccgaa cagcacgatg gcgggaatgc cttcgagcac
      421 gctgcccagc acggcgaagg ccaggatcga cacggcgagg aagccggcct tcccgcccgg
      481 caccgtcgcc atcgccgcga cgagcttgcc ggaaaagccc gattgcgtga gtgcccaggc
      541 catcgcggtc gccgtgccga tgatcagcag gatcgcgccc gagagcgccg ccgtgtccac
      601 cagcatcgga taaaggcgcc gccacgcgaa ttgccgatag atcaggagac cggcgagcac
      661 ggcataggcg atgccgatgg tcgagacctc ggtggcggtg gcgatcccct cgaccacgac
      721 ggcgcggatg aggaacggca ggaccaatgc cggcagcgcc acgacgaagc ggcgcgcgat
      781 ctggcgcggg ctcggcctgg cctcgccgcc gggatcgtcg ccgcgcgagc ggaaataaac
      841 gaggacgacg agcgccagcg ccagaaccag cgccgggacg aggccgccat tgaagagcgc
      901 ggcgatcgag acgccggtga ccgagccgat ggtgaggagc acgatgctcg gcggaatcgt
      961 ctccgacatg gcgcccgccg cggccagctg ggccaccagc tcgcccggcg cggcgccacg
     1021 ctgcttcatg gcgggaaaga gcgccggagc cacggctgcc atgtcggcgg ccttcgagcc
     1081 cgagatcccc gagacgaggt acatcgccgc gagcagcaca taagagaggc cgccgcgcac
     1141 gccgcccagc aacgcggcaa ggaaatcgat catggcgcgg gcaagcccgg tcatctcgat
     1201 gagcacgccg aggaaaatga agagcggcac ggcgagcagg atgagatgcg acatgccttc
     1261 atccatgcgg cccatgacga cgatcagcgg cacatgggtc atgagcccga gataagccag
     1321 cgtcgcgatg ccgaaggtga aggcgatcgg cacgccgatg acgatcgccg cggcgaccag
     1381 acccacgaag aagacagcga ggttgtagct gccgagcgcg agcagtgccg gctgcgcgag
     1441 atagagcgcc cccgccacgc ccgccgtcac gaggagcgcg acgacgagct gcgcgaggcc
     1501 ggtgtacgcg gcgaggcgca acagcgcgtt cgccatcatc aacaaagcgc cgacggcgat
     1561 cgcggcggcg cgaaagctgt tgtggatctc gaggccgggc gtgaggatgg cccattcctc
     1621 cgcgacatag tcgagagacg gccgcatgat cagcagcatg aaggtcatca cgacgagagc
     1681 ggcgacggtg tcggcgaggc cgcgccagcg ctgcggcaaa cggttgacga tggcggtgag
     1741 gcgcatgtgc tcgccgcgcc gcaaggcgat caccgaaccg agcatggcaa gccagagaaa
     1801 gagaatggag gcgagctcat cgctccaggt gagcgggcta cggaagacgt agcgcgagac
     1861 cacgcccgct gacagcacga cgatctcggc ggcgacgagc gctgcggccg gcggctccgt
     1921 ggcccaggcg acgccgcgat caaggtgctg cgcgatggcg cgcgcggtcg tcgaagaggg
     1981 agcagcggac cccccacacc ggccctcccc ctcaaggggg gacgtggaag gattgtgctc
     2041 gctcaaacat tccctcccgc cttgaggggg agggccaggg tcggtgatgc tcacgccagg
     2101 gggcccgcat atttctccag aacggcccag gcgtccgcgc cgtatttctc gcgccattcg
     2161 cggtagaagc cggcttcttt aaggacggcg cggaaggcct cggtgtcggg cgcgttgaag
     2221 atcagacctt tcttctcgag gtcgccttgg agcgtgtcgt tgagaaggcg gatgtcgtcg
     2281 cgctgggcca gggccgcggc gttccaatga tgcgtcacga tctcctggag atccttcggc
     2341 aacgcttccc aggccggc
//
LOCUS       DASYNS010000083         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2986167, whole genome shotgun sequence.
ACCESSION   DASYNS010000083 DASYNS010000000
VERSION     DASYNS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2986167"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VGP50_02555"
     CDS             <1..499
                     /locus_tag="VGP50_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEV8026280.1"
                     /translation="AERLRALREYGWRARYVSDIAGMNSRLDELQAAILRVKLAALDA
                     DNARRAAMAAAYDAGLAGLPLRLPARQAGTSHVFHQYVIRSAERDRLRAALTERGIGT
                     NIHYPVPVHLQPAYRGRVAVGPGGLAESERAAREVLSLPMYPQLDAAQVARVVAALRD
                     GPSSG"
     gene            590..>1682
                     /locus_tag="VGP50_02560"
     CDS             590..>1682
                     /locus_tag="VGP50_02560"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEV8026281.1"
                     /translation="MRSILSNAVWAFRNLFAGLRLTLPVPVGRQSFRFSADQALILLL
                     VACGAALLDGYFMSESGARLSWYAWPILAARCFFALLLYYLVARLQGALETLPAIAVT
                     LFGATAMLDLYQGLIYWIVRAHWTVSRSDAGFWWAAWAVWALTLAWVSATIFRGVRLV
                     YETRWLRSGLLAAMIVVGSVATQWVAPGRFWYVPVAEADDDSTDPRVDTEHTYYAQRS
                     LLTKTLSALAPSRRGVSELYFLGFAGTSTEDVFMKEARVAQALFDERFDTRRRSLILV
                     NNPQTVDELPIASVSNLDLALAGIAKRMDVEKDVLFLYLTSHGARHVFSVSFPELSLG
                     DLGDRQLKIMLDRSGIKWRVLVISACYSGS"
BASE COUNT          236 a          566 c          553 g          327 t
ORIGIN      
        1 cgccgagcgc ttgcgggcgc tgcgcgaata tggctggcgc gcgcgctatg tcagcgacat
       61 cgccggcatg aactcgcggc tcgacgagct gcaggcggcc atcctccgcg tcaagctcgc
      121 cgcgctcgat gccgacaacg cgcgccgcgc ggcgatggcc gcggcttacg atgcggggtt
      181 ggccggtttg cctctgcggc tcccggcgcg ccaggcgggc acaagccacg tcttccacca
      241 atatgtcatc cgctcggccg agcgcgaccg cctgcgcgcc gcgctgaccg agcgcggcat
      301 cggcacgaat attcactatc ccgtgccggt tcatcttcag ccggcctatc gcgggcgcgt
      361 cgcggtcggg cccggcggtc tcgccgagag cgagcgggcg gcgcgcgagg tgctgagcct
      421 gccgatgtat ccgcagctcg acgctgcaca ggtggcgcgg gtcgtcgcgg cgttgcgcga
      481 cggccccagc tcgggttgat acccgtttcc cggcattcga tcgattaaga gatcgaccac
      541 aagtcgaccg ctagcatggt cagaggccgg gccatgggga aaccaaagca tgcgcagtat
      601 tttatcgaat gcggtctggg cgttcagaaa tctctttgcc ggtcttcgcc tgaccttgcc
      661 ggtcccggtc ggccgccaat catttcgctt cagcgccgac caggcattga tcctccttct
      721 ggtcgcatgc ggcgcggcgc tcttggacgg ctacttcatg agcgagagcg gcgcgcggct
      781 cagctggtat gcctggccca tcctggcggc gcggtgcttc ttcgcactgc tgctctatta
      841 tctcgtcgcc cggctgcaag gcgcgctcga gacgttgccg gccatcgccg tgacgctttt
      901 tggcgccacc gccatgctgg atctctacca ggggctcatc tactggatcg tcagggccca
      961 ttggaccgtc agccggtcgg acgcgggctt ctggtgggct gcatgggctg tatgggcgct
     1021 caccctggcc tgggtcagcg ccacgatctt tcgcggcgtg cgtctcgtct acgaaacgcg
     1081 atggctgcgc agcggtctgt tggcggccat gatcgtcgtc gggtccgtgg cgacccagtg
     1141 ggtggcgccc ggccgtttct ggtatgtgcc ggtcgccgag gccgacgacg acagcaccga
     1201 ccctcgcgtc gacacggaac acacctacta cgcccagcgc tccttgctga cgaagacgtt
     1261 gtcggcgctg gcaccgtcgc ggcgcggcgt gagcgagctt tatttcctcg gctttgccgg
     1321 gacatcgacc gaggacgtgt tcatgaagga ggcgcgcgtc gcccaggcgc tgttcgacga
     1381 gcgcttcgac acgcgccgcc gctcgctgat cctcgtcaac aatccgcaga ccgtggacga
     1441 gctgcccatt gccagcgtgt ccaacctgga tcttgcgctc gccggcatcg ccaagcggat
     1501 ggatgtcgag aaagacgtcc tgttcctcta cctcacgtcg cacggcgcgc ggcatgtgtt
     1561 ctccgtctcg tttccggagc tgtcgctggg cgatctcggg gatcggcagc tcaagatcat
     1621 gctcgaccgc tcggggatca aatggcgcgt cctcgtcatc tcggcctgtt attccggcag
     1681 ct
//
LOCUS       DASYNS010000084        13225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_489381, whole genome shotgun sequence.
ACCESSION   DASYNS010000084 DASYNS010000000
VERSION     DASYNS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 13225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13225
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489381"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="VGP50_02565"
     CDS             complement(<1..1212)
                     /locus_tag="VGP50_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV8026282.1"
                     /translation="MDIRNIAIIAHVDHGKTTLVDQLLRQSGAFRANQQVMERALDSN
                     DLERERGITILAKCTSVQWEGTRINIVDTPGHADFGGEVERILSMVDGVLVLVDAAEG
                     PMPQTKFVVDKALRKGLRPIVIINKVDRSDARVHAVHDEIFDLFAALDATDEQLDFPT
                     LYASGRAGWAVTDLAEPHKDLRPLFDLIVRHVKPPVADLDKPFAMLVTTLESDSYLGR
                     VLTGRIHSGVARMNMPVKSLSRDGKVIEQARLTKLLAFRGLDRRPIEEAEAGDIIAIA
                     GLGETTVADTLCAPEVTEPLPATPIDPPTLAMTFSVNDSPLAGREGSKVTSRMIVERL
                     HREAEGNVAIRVRENDDKDAVEVAGRGELQLGVLIETMRREGYELAISRPRVLLKTDP
                     ATGQRLEPIEEI"
     gene            complement(1407..2423)
                     /locus_tag="VGP50_02570"
     CDS             complement(1407..2423)
                     /locus_tag="VGP50_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEV8026283.1"
                     /translation="MEHALSATRIWRQLHQSALHEQRSAEATLAPALAAMLPPRQAIL
                     VTGGTGFIGTRLCAILAEAGHRVTVLTRDPRNGRKFKGRVTLIDNLSTLGRDTPFDAV
                     VNLAGEPMVARRWTPERRRLLCESRVETTRSVVKLIARSRRKPAVLVSASAVGFYGID
                     DAATFTESSPGLPAFTHDVCRAWEAEALEAERLGVRVCLLRTGIVLGAEDGALARMLP
                     VFALGLGGRIGSGRQWMSWIHLDDIVGLIIHAMVVETVRGPLNATAPEPVRNADFAAA
                     LGRALHRPALLPIPAFALRLALGQLADEVLLGGQRVLPQQAQETGYQFLYPTIEEALK
                     EIVG"
     gene            complement(2427..3083)
                     /locus_tag="VGP50_02575"
     CDS             complement(2427..3083)
                     /locus_tag="VGP50_02575"
                     /inference="COORDINATES: protein motif:HMM:NF025135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HEV8026284.1"
                     /translation="MRCGSQEEIERVPARLEPDPSFRALLGDLAWRRLPSAVRERFAW
                     KPTPGAEIRYRGVMAVVRRSTIGALMAQLCRLIGTPLAPYGGDDVPVTVVLRSDHKSG
                     GIVWDRVYAFSGRAAVHCASIKRVSGRDGLLECVGGGVGMWLALTECQGELHFRSTGY
                     FWECGGLRLRVPSWLTPGALHVVHADLGAGRFRFRIAVRHRFLGETFFQDGIFAADRR
                     "
     gene            complement(3138..3671)
                     /locus_tag="VGP50_02580"
     CDS             complement(3138..3671)
                     /locus_tag="VGP50_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV8026285.1"
                     /translation="MRLTPVMERYVLHWGEMGARWGVNRSVAQIHALLYLSPQPLTAE
                     EIADTLALARSNVSTSLRELQGWGLVSLSHALGDRRDHFEAKTDLWDLLLTIVEERKR
                     REVDPTLTMLRQCVLEADDDKETDPEVKARIARMLTFLETLAIWYAQMRGLQKSTLVA
                     LMKMGARVAKAVGLVKA"
     gene            complement(3763..4533)
                     /locus_tag="VGP50_02585"
     CDS             complement(3763..4533)
                     /locus_tag="VGP50_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026286.1"
                     /translation="MTPPDEFLVVDSEKFDLAATERLFELFAVPRGEREEVWLIAFRR
                     AAWNASVVLPTPQVMTGPDGFPYLRLVLPPPGEPFQSNCLSNVCAFCAERGLGVAYFA
                     SEADPPIALQYAIPTGVIESLCRFGSWAGDPLDGEPPPEDDRRPDAANARQALVGAPS
                     ADYLPPHTARLLGRHLIERWKIPEPRVALLVAPQLRPSRNLVIGRKRSEFADEVTADR
                     QMEALLWYFPPGRSLVLMPEDWPEDRLTPLSDLWDGAV"
     gene            complement(4592..5584)
                     /locus_tag="VGP50_02590"
     CDS             complement(4592..5584)
                     /locus_tag="VGP50_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEV8026287.1"
                     /translation="MATASLDVVGIGNAIVDVIAQADDGFLSREGLTKGAMQLIDARR
                     AEALYAAMGPGIETSGGSAGNTMAGIASLGGKAAYIGKVAEDQLGGVYRHDMRAIGVR
                     FDTAPLRQGPPTARCLILVTPDAHRTMNTFLGACVELGPDDVAADLVQAAQVTYLEGY
                     LFDPPRAKQAFRKAAELAHAAGRKVSLTLSDSFCVERYRDEFRALVEGHIDILFANES
                     EIGALYETDFDHALAAVRGKCEIAVLTRSERGSVVVTADAVHRIAADEIERVLDTTGA
                     GDLYASGFLFGLTHGRDLAACGRLGSLCAAAILGHYGPRPEASLERLAKTRGLL"
     gene            5695..6798
                     /locus_tag="VGP50_02595"
     CDS             5695..6798
                     /locus_tag="VGP50_02595"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV8026288.1"
                     /translation="MHNLLTDVASITVGHATDLALGSGVTAVLFDQPAVAAVSMLGGV
                     PGERQVAMLEDGMTLDSVDAIVLAGGSSFGLDAAGGVQAALRERGRGLALGTVRVPIV
                     SEAIIFDLANGGNKDWGRFAPYRELGYEAAAAAADTFTLGSVGGGTGATTAIVKGGLG
                     SASARTAAGHIVAALVVVNAVGSPLIGDGPWFWAAPYEQSGEFGGRGWPARIDPSLRL
                     KGIASGTSTTIGLVATDASLTKGQAKRLAIMAHDGLARAVLPAHAPLDGDTIFAAATG
                     MRALAILRENSPSLAIPRRFAWRVRLAAASTKQPRFPSSPGSAPGASCSPERAGAGNA
                     KGYVWSPGAAALRDFRLYISAEPGKAPLRTSAA"
     gene            complement(6702..7550)
                     /gene="panB"
                     /locus_tag="VGP50_02600"
     CDS             complement(6702..7550)
                     /gene="panB"
                     /locus_tag="VGP50_02600"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEV8026289.1"
                     /translation="MSQHVVSSRLTVPEIRGRKGKEKLVCLTAYTAPMAAILDPVVDL
                     ILVGDSLGMVVHGLPTTVGVTLDMMILHGRAVMRGSTHALVVVDLPFGSYERGPQQAH
                     ESAVRVMQETGCQAVKVEAGEGVPETIGFLVRRGIPVVGHVGLRPQAANVDGGFRAKG
                     RSASEREAVLAEARAVDQSGAFAMVVEGVAGELADEITRQVAIPTIGIGASASCDGQI
                     LVTDDMVGLFDWTPKFVRRYGAMRELVSQAVQAYAADVRSGAFPGSAEMYSLKSRKAA
                     APGDQT"
     gene            8020..8709
                     /locus_tag="VGP50_02605"
     CDS             8020..8709
                     /locus_tag="VGP50_02605"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HEV8026290.1"
                     /translation="MLTAFRIAIDDAFAPEQRRILVLSMGIAVALLLALWLGATALLE
                     EARFAGIGWLDAVIIVLGSLAALVVAWLLFPAIALLVLGLFLGSVVTAAERRHYPALG
                     PPRPLELGATVASALRLLLLAVLVNIILLPVYLIPGINLFVYYGLNGYLLGREYFELV
                     ALRRVDGAAAQVMWRRHRGRLILVGVIIVFLLSLPLINLVASVVGAAFMLHVFEELRR
                     RAPTETFAVRL"
     gene            8782..9441
                     /locus_tag="VGP50_02610"
     CDS             8782..9441
                     /locus_tag="VGP50_02610"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV8026291.1"
                     /translation="MFGRKKLQDHDTNFSGFSDELGIPAKPTPRSAGITPSSGAARQP
                     TASTARPAASEAAKPADAYKVTSDAARSSSTPVPARRDPETRKLIVGREISLSGEITA
                     CDRLVVEGSVSANLANCRDIDIAESGLFKGSASIEDAEVRGRFEGTLNVRRRLLIRAS
                     GRVTGSVRYGQIEIECGGQISGDVQAQPMAESNEAPARAAEPASAARPNVVPLADDAR
                     H"
     gene            complement(9438..9722)
                     /locus_tag="VGP50_02615"
     CDS             complement(9438..9722)
                     /locus_tag="VGP50_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026292.1"
                     /translation="MPVKSAGIAGGGGAGKPAGGTTAAAVAPTSDEGDIVNVGVGRPQ
                     QAVSAAMPQNTTKAKAPRERSHSLAILPPKTVAAFCDLIAPCSVSEAPVA"
     gene            9721..9966
                     /locus_tag="VGP50_02620"
     CDS             9721..9966
                     /locus_tag="VGP50_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026293.1"
                     /translation="MGAAEVLALLGSPDFRRSEPPAELWQYRAADCILDVFLYRDPGG
                     YRVVHAETRRRRQIVATGNGCSAGPAGFVSHGRQSRL"
     gene            complement(10498..11205)
                     /gene="nth"
                     /locus_tag="VGP50_02625"
     CDS             complement(10498..11205)
                     /gene="nth"
                     /locus_tag="VGP50_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014625878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV8026294.1"
                     /translation="MTPQDVDEFFRRLAKERPDPRSELDYINPYTLLVAVVLSAQATD
                     AGVNKATEKLFKVADTPQKMVALGEARLRDFIKTIGLFNTKAKNVIALSEKLIAEDGG
                     EVPRTWEALEALPGVGRKTANVVLNVAFGQNTIAVDTHVFRVANRTGMAPGATPLLVE
                     VELERVVPDRWKHNAHHWLILHGRYVCIARKPRCEACVVNDLCAFPDKTSSVPTPVRK
                     TAAKKRGVRATARRRRA"
     gene            11282..11791
                     /locus_tag="VGP50_02630"
     CDS             11282..11791
                     /locus_tag="VGP50_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEV8026295.1"
                     /translation="MSEPRPANSPPVLYDAELAPHCSLPPRGFAVLMLVLAGASGTVG
                     MAFVLRGAWPVTPFFGLDLLLVYLAFRMSYRQARRREALRLTEENLTVERTDPKGGRR
                     HWHFHPFWLRVVLEEKDEHSNRLVLASHGRSLVVGSFLGPDERRDVARDLTDALGRWK
                     TRLPADRDA"
     gene            complement(11795..12595)
                     /gene="dapB"
                     /locus_tag="VGP50_02635"
     CDS             complement(11795..12595)
                     /gene="dapB"
                     /locus_tag="VGP50_02635"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV8026296.1"
                     /translation="MRIGITGCAGRMGRMLVAETQAVAGLRLAGGSEAPGSSSLGRDI
                     GELAGIGPVGIAATADLAALFAASDAVIDFTVPAASATHAEQAAKSGKALVIGTTGLD
                     AKQAKTIETAARHVPIVWAPNMSPAVTMLLSLVEEAARRLDDGYDIEVLEMHHHHKVD
                     APSGTALALGRAAAAGRGIDLGARSQRVRDGHTGARTRGDIGFATLRGGDVVGDHVVI
                     FAGEGERLELAHRATSRRIFALGALRAARWVVAQKPGLYGMKDVLGLK"
     gene            12728..>13225
                     /locus_tag="VGP50_02640"
     CDS             12728..>13225
                     /locus_tag="VGP50_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEV8026297.1"
                     /translation="MALLRSVATVGGYTMISRVLGFLREMLTAAFLGAGPLADAFFVA
                     LRLPNMFRSLFAEGAFSAAFVPLFAGRLAQEGRPAARRFAEDTLAVLLVALLLFLVAG
                     EILTPWILDLLAPGFRADPEKFARAVDLTRIMFPYLLFISLTALEGGVLNSLERFAAA
                     AATPVL"
BASE COUNT         2124 a         4442 c         4420 g         2239 t
ORIGIN      
        1 aatctcctcg atgggctcga ggcgctggcc ggtggcggga tcggtcttga ggaggacgcg
       61 cgggcgcgag atcgcgagct catagccctc gcgtcgcatg gtctcgatca acacgcccag
      121 ctgcagctcg ccgcggccgg cgacctcgac ggcatccttg tcgtcgttct cgcgcacccg
      181 gatcgcgaca ttaccctcgg cctcgcgatg caggcgctcg acgatcatgc gcgacgtgac
      241 cttgctgcct tcgcgtccgg cgagcgggct gtcattgacc gagaaggtca tggccagcgt
      301 cggcggatcg atgggtgtcg ccggcagcgg ctccgtcact tcgggagcgc agagcgtatc
      361 ggcgacggtc gtctcgccga gaccggcgat ggcgatgatg tcgccggctt cggcctcttc
      421 gatcggccgg cgatcgaggc cgcggaaggc caggagcttg gtgagccgcg cctgctcgat
      481 gaccttgccg tcgcgcgaca gcgacttcac cggcatgttc atgcgggcga cgccgctgtg
      541 gatgcgcccg gtcagcacgc ggcccagata agagtcgctc tcgagcgtgg tgacgagcat
      601 ggcgaagggc ttgtcgaggt cggcgaccgg cggcttcaca tggcggacga tgaggtcgaa
      661 gagcggccgc aggtccttgt gcggctcggc aagatcggtg actgcccagc cggcgcggcc
      721 cgaggcgtag agcgtcggga agtcgagctg ctcatcagtg gcgtcgagcg ccgcaaagag
      781 atcgaagatc tcgtcatgga cggcatgcac gcgcgcgtcg ctgcggtcga ccttgttgat
      841 gatgacgatc gggcgcaggc ccttgcgcag cgccttgtcg acgacgaatt tggtctgcgg
      901 catcggccct tcggccgcgt cgacgaggac gagaacgccg tccaccatgc tgaggatgcg
      961 ctcgacctcg ccgccgaaat cggcatggcc gggcgtgtcg acgatgttga tgcgcgttcc
     1021 ttcccactgc acggacgtgc atttggcaag gatggtgatg ccccgttcac gctccaggtc
     1081 gttcgaatcg agggcgcgct ccatcacctg ctggttggcg cggaaggcgc cgctctgacg
     1141 cagcaattgg tcgaccagcg tggtcttgcc atggtcgaca tgggcgatga tggcgatatt
     1201 gcgaatgtcc atgggcgcgc agaatgacaa tgaagggtta atacgatatg acagcgttag
     1261 atggggatga gcgggtcaaa ccgcaatctt cgatctctgc ggcgcagtct tatagccgac
     1321 atcgcgtcgg cggtcgacaa attcctgcgc agatgcgcac tattgccaaa cataggacga
     1381 cgttcaacgg tgcgcggcgc gcgcgttcat ccgacgatct ccttcagcgc ctcctcgatc
     1441 gtcgggtaga ggaactggta ccccgtctcc tgcgcctgct gcggcaatac ccgttggccg
     1501 cccagcagca cctcgtcggc aagctggccg agcgccaggc gcaaggcaaa ggccgggatc
     1561 ggcagcagcg ccgggcggtg gagcgcgcgg ccgagcgccg ccgcgaaatc ggcattgcgc
     1621 accggctcgg gcgcagtggc gttgagcggg ccgcggaccg tctcgaccac catggcgtgg
     1681 atgatcaggc cgacgatgtc atcgagatga atccacgaca tccattggcg cccgctgccg
     1741 atccggccac ccagccccaa cgcgaagacc ggcagcatcc gggcaagcgc gccgtcttcg
     1801 gcaccaagga cgatgccggt gcgcagcagg cagacgcgca cgcccaggcg ctctgcctcg
     1861 agcgcctctg cttcccacgc cctgcagacg tcgtgcgtga aggcgggcag gcccggcgag
     1921 ctctccgtaa aggtcgctgc gtcgtcgatg ccgtagaaac cgacggcgga ggcgctcacc
     1981 agcaccgcgg gcttccggcg gctgcgggcg atcagcttca cgacggagcg tgtcgtctcg
     2041 acgcgactct cgcagaggag gcgccgccgc tccggcgtcc agcgcctcgc caccatcggc
     2101 tcgccggcga ggttgacgac ggcatcaaag ggcgtgtcgc ggccgagcgt cgagagattg
     2161 tcgatgagcg tgacgcgccc tttgaatttt cggccgttgc gcggatcgcg cgtcaggacg
     2221 gtcacgcgat gtccggcttc ggcgaggatg gcgcagagac gcgtgccgat gaatccggta
     2281 ccgccggtga ccaggatcgc ctggcgcggc ggcaacatcg cggcgagcgc cggcgcgagc
     2341 gtcgcctccg ccgatcgctg ctcatgcagc gcgctctgat gcagctgccg ccatatccgc
     2401 gtcgcagaca gagcgtgttc catgactcac ctcctgtcgg ctgcgaaaat gccgtcctgg
     2461 aagaaggtct cgccgagaaa gcgatgacgg acggcaatgc ggaagcggaa acggcccgcg
     2521 ccgagatcgg catggaccac gtgcagcgcg ccgggggtga gccaggaggg tacgcgcagg
     2581 cggagcccgc cacactccca gaaatacccg gtgctgcgga agtgaagctc accctgacat
     2641 tcggtgagcg ccagccacat gccgacaccg ccgccgacgc attcgagaag cccgtcgcgg
     2701 ccgctcaccc gcttgatcga ggcgcaatgc acggcagcgc ggccggaaaa ggcgtagacc
     2761 cggtcccaga cgatgccgcc gctcttatgg tcggagcgca ggacgaccgt cacgggcacg
     2821 tcgtcgccgc cataaggggc gagcggcgtg ccgatgagcc gacagagctg agccatcagc
     2881 gcgccgatgg tcgagcggcg caccaccgcc attacgcccc gataccggat ctcggcgccc
     2941 ggagtcggct tccaggcgaa gcgctcgcgc acggccgacg gcagccgccg ccaggccaag
     3001 tcgcccagaa gcgcccgaaa gctcggatcg ggctcgaggc gcgcaggaac gcgctcgatc
     3061 tcttcctggc tgccgcaccg catttccgcc tccgcgttct gaatgttctg ttcaaacaga
     3121 aatcagcgga caaaaaatta tgccttcacc agccctacgg ccttcgccac cctggcgccc
     3181 atcttcatca gcgcgaccag cgtcgatttc tgcaggccgc gcatctgcgc gtaccagatc
     3241 gccagcgtct cgagaaaggt cagcatgcgc gcgatccgcg ccttgacctc gggatcggtc
     3301 tccttgtcgt cgtccgcctc gagcacgcat tgacgaagca tggtcagcgt cggatcgacc
     3361 tcgcggcgct ttcgttcctc gacgatggtg agcagcagat cccagaggtc ggtcttcgcc
     3421 tcgaaatggt cgcgccggtc gcccagcgca tgactgaggc tgaccaggcc ccagccctgc
     3481 aactcgcgca aggacgtgct gacattcgag cgcgccagcg ccagggtgtc ggcaatctcc
     3541 tcggcggtga gcggctgcgg cgagaggtaa aggagggcgt ggatctgcgc caccgagcga
     3601 ttgacgcccc aacgggcccc catctcgccc cagtgcagca cataacgctc catgacgggc
     3661 gtcaggcgca ctgcattctc ctatttctgt accgacagaa attacagtcg aaaatgccgg
     3721 cgctgtcaag cggaaacgcg gccgcgatcg tgggccaccg cctcagacgg caccgtccca
     3781 gaggtcgctg agcggcgtca gccggtcctc gggccagtct tccggcatca ggacgagcga
     3841 ccggcccggc gggaaatacc acagcagcgc ctccatctgc cgatcggccg tgacctcatc
     3901 ggcgaattcc gaacgcttcc gcccgatgac caggtttcgc gagggacgca gttgcggcgc
     3961 gacgagaagg gcaacgcgcg gctccgggat cttccaccgt tcgatcagat ggcgcccgag
     4021 gagccgggcc gtgtgcggcg gcaagtaatc ggcggaaggc gcgccgacca acgcctgtcg
     4081 agcgttggcc gcgtccggcc gccggtcgtc ttccggcggc ggctcgccgt cgagcggatc
     4141 accggcccag cttccgaacc ggcatagcga ctcgatcacc cctgtcggaa tcgcgtattg
     4201 cagcgcgatc ggcgggtccg cttcgctggc aaagtaggcg acgcccaagc cgcgctcggc
     4261 gcagaaagcg cagacattcg acaagcagtt ggactggaac ggctcgccgg gcggcggcag
     4321 gacaagccgc agatagggga aaccatctgg cccggtcatg acttgaggcg tcggcagcac
     4381 gacggacgcg ttccatgcgg cgcgccggaa ggcgatcagc caaacctcct cgcgctcgcc
     4441 gcgaggtacg gcgaagagtt cgaagagccg ctccgtggca gcgagatcga atttctccga
     4501 atcgacgaca agaaactcgt cgggcggcgt catgctcgcg ctcgagcacc gcgcggtacg
     4561 cgatctttct ccggattgtg cttccgcacc ctcagaggag acccctggtc ttggcgaggc
     4621 gttcaagcga ggcttcgggc ctggggccgt agtggccgag aatcgcagcc gcgcagaggc
     4681 tgccgagccg gccgcaggcg gcgaggtcgc ggccatgggt gaggccgaag aggaatccgc
     4741 tcgcatagag gtcgccggcg ccggtcgtgt cgagcacgcg ctcgatctcg tcggccgcga
     4801 tgcgatggac ggcatcggcg gtcaccacga ccgagccgcg ctcgctgcgt gtgagcacgg
     4861 caatctcgca tttgccgcgc acggcggcga gcgcgtggtc gaaatcggtc tcgtagagcg
     4921 cgccgatctc gctttcattg gcgaagagaa tgtcgatatg gccttcgacg agcgcacgga
     4981 actcgtcgcg atagcgctcg acgcagaagg agtccgacag cgtgaggctg accttgcggc
     5041 cggcggcgtg ggcaagctcg gccgccttgc gaaaggcctg cttggcgcgc ggcggatcga
     5101 agagatagcc ctcgaggtag gtcacttgcg ccgcctggac gaggtcggcc gcgacgtcat
     5161 cgggaccgag ttcgacgcac gcgcccagga acgtattcat ggtgcggtgg gcgtcgggcg
     5221 tgaccaggat caggcagcgc gccgtgggcg gtccctggcg caacggtgcc gtgtcgaaac
     5281 ggacgccgat cgcgcgcatg tcgtggcggt agacaccgcc gagctgatcc tcggccactt
     5341 tgccgatata ggcggccttg ccgcccagcg acgcgatgcc ggccatggta ttgcccgcag
     5401 aaccgccgga cgtctcgatc cccggtccca tggccgcgta gagcgcctcg gcgcgccggg
     5461 cgtcaatgag ctgcatggcg cccttggtca acccctcgcg ggagagaaag ccgtcatctg
     5521 cctgggcgat cacgtcgacg atcgcattgc cgatgccgac gacgtcgagt gaagctgtcg
     5581 ccattgagat cctgtccggg tcacgccgcg cgcggctcaa gtataaggag gagcggcgag
     5641 cgggcaagat cgccggacgc gctttatagt aagcgccgca cgaggaggac accgatgcac
     5701 aatctgctga ccgatgtcgc cagcatcaca gtgggtcatg cgacggactt ggcgctgggc
     5761 tcgggcgtca ccgccgtgct tttcgaccag ccggcggtcg ccgcggtgtc gatgctcggc
     5821 ggcgtgcccg gcgagcgtca ggtggccatg ctcgaggacg gaatgaccct cgattccgtc
     5881 gatgcgatcg tgctcgcggg cggctcttcc ttcgggctcg acgcggcggg tggcgtgcag
     5941 gcggcgttgc gcgaaagagg ccgcgggctg gcgctcggca ccgtgcgcgt gccgatcgtc
     6001 tccgaggcga tcatcttcga tctggccaat ggcggaaaca aggactgggg ccgtttcgcg
     6061 ccttatcgtg agctcggcta cgaagcggca gcggcggccg ccgatacctt caccctgggc
     6121 agcgtcggcg gcggcaccgg cgcaactacg gcaatcgtga aaggcggcct cggctcggcg
     6181 agcgcgcgca ccgccgccgg tcacatcgtc gccgccttgg tcgtggtcaa cgccgtggga
     6241 tcgccgctga tcggcgacgg tccatggttc tgggcggcac cctatgagca gagcggcgaa
     6301 ttcggcggcc gcggctggcc cgcgcggatc gatccttcac tgcggcttaa aggcatcgcc
     6361 tccggcacca gcaccacgat cggcctggtc gccacggatg cgtccttgac gaaaggccaa
     6421 gcgaagcggc tcgcgatcat ggcccatgac gggctcgcac gggcggtcct gccggcacat
     6481 gcgccgctcg acggcgacac gatattcgcg gcagcgaccg gcatgcgcgc gcttgcgatc
     6541 ctgcgcgaga actcaccgag cttggctata ccgcggcgct ttgcctggcg cgtgcgattg
     6601 gccgcggcat ctacgaagca accgcgcttc ccgtcgagtc cgggcagcgc tcctggcgcg
     6661 agttgttcgc ctgaacgcgc cggcgccggc aatgccaagg gctatgtctg gtctccgggc
     6721 gcggccgcct tgcgcgactt caggctgtac atctcggcag agccgggaaa ggcgccgctt
     6781 cggacgtcgg cggcataagc ctggacggcc tgactcacca gctcgcgcat ggcgccatag
     6841 cggcggacga atttcggtgt ccagtcgaag aggccgacca tgtcgtcggt gaccaggatc
     6901 tggccatcgc agcttgccga cgcgccgata ccgatcgtcg ggatggcgac ttgccgtgta
     6961 atctcatcgg cgagttcgcc ggcaacgcct tcgacgacca tggcgaaggc gccggactgg
     7021 tcgacggcac gagcttcggc gagcaccgcc tcccgctcgc ttgcagagcg gcccttggcc
     7081 ctgaatccgc cgtcgacatt agccgcctgc ggccgcaagc cgacatggcc aaccacgggg
     7141 atgccgcggc gaacgaggaa tccgatggtt tcgggtacgc cctcgccggc ttcgaccttg
     7201 acggcctgac atcccgtttc ctgcatgaca cggaccgccg actcatgagc ctgctgcggg
     7261 ccgcgctcat agctgccgaa gggcaggtcg accacgacca aagcatgggt cgacccgcgc
     7321 atcaccgcgc ggccgtgcaa gatcatcatg tcgagcgtca cgcccaccgt ggtcggcaag
     7381 ccatggacga ccatgccaag cgaatcgccg accaggatca aatcgacgac gggatcgaga
     7441 attgctgcca tcggcgccgt ataggcggtc aggcagacga gcttctcctt gcccttgcgg
     7501 ccccgaatct cgggaaccgt caggcgcgat gatacgacgt gctgggacat gtggtcctcc
     7561 tgaccaggcg cgcggcgacc gctcgcggcg gcgccgctcc aaaacagctc ggaagaacag
     7621 aagccggtag gcttgaccct gccttggctc aaaaaatagc ctatttgcca gacgaagcaa
     7681 actcgtcgct tccgcaagtc ccggccgcga tccgcgaatg gctcggttgg ttgacagggc
     7741 cggcccgccc gtggactgtg ccgtcgcaga tcgctctggt ttgccgcggt gggcctcctg
     7801 ccacggtttg gcagtgcttc tgctctatgc tgcgctgcgc cgcggatcgg ttggcgtcgt
     7861 ctctcctctt gtagctacct gcccgcgcgt gaccttggta ttgagccgta tccttctgaa
     7921 cgaagaacgc ggcggcggcg gtcttgtcgc cggtgtcgcc gtcaccgtcg cgggcgccgt
     7981 tttgatgctc ctcgcttagc acacccagga tcgttgccga tgctcaccgc cttccgtatc
     8041 gccatcgacg acgcattcgc tccggagcaa cggcgcatcc tggtcctgag catggggatc
     8101 gcggtggccc tcctcctcgc gctctggctc ggcgcgacgg cgctcctcga ggaggcccga
     8161 ttcgccggga tcggctggct cgacgccgtg atcatcgtgc tcgggagcct cgcggcgctg
     8221 gtggtcgcct ggctcttgtt tccggccatt gcgctgctgg tgctggggct ctttctcggc
     8281 agcgtcgtca cagccgcaga gcgccgccat tatcctgcgc tcggaccgcc ccgccctctc
     8341 gaattgggcg cgacggtcgc gagtgcgctc cggcttctcc tgctcgcggt gctggtcaac
     8401 atcatccttc tgccggttta cctgatcccg ggcataaacc tctttgtcta ctatggcttg
     8461 aacggttatc ttctcgggcg tgaatatttc gagctggtcg cgctccggcg ggtggacggg
     8521 gcagcggcgc aggtgatgtg gcggcggcat cgcggccggc tgatcctggt cggggtgatc
     8581 atcgtcttcc tgttgtcgtt gcctctgatc aacctggtgg catcggtcgt cggtgccgca
     8641 ttcatgctcc atgtcttcga ggagctgaga cggcgtgcgc cgaccgaaac tttcgcggtt
     8701 cgcctctgag tatggttaat cgaagattaa tcactcggcg cgagccttcg gctccgacct
     8761 cacaaaccgt caggtgctct catgttcggc cgtaagaagc tgcaagacca cgacacgaat
     8821 ttctccggtt tctccgacga gctcggcatt cccgcgaagc cgacgccgcg ctccgcaggg
     8881 attaccccct cgagcggagc tgcgcgccaa cccacggcgt caacggcgcg gcccgcggcg
     8941 agcgaagcgg caaagcctgc cgacgcctac aaggtcacga gtgatgctgc tcggtcgtcc
     9001 agcacccctg tgccggcgcg gcgcgatccc gagacgcgga agctcatcgt tggccgcgag
     9061 atctcgctct cgggcgagat cacggcctgc gaccgcctcg tggtcgaagg cagtgtcagc
     9121 gccaacctgg ccaattgccg cgacatcgac atcgccgaga gcggtctctt caagggatcg
     9181 gcgtcgatcg aggacgcgga ggtgcgcggc cgcttcgagg gcacgctcaa tgtccggcga
     9241 cgcctgctca ttcgcgccag cggccgcgtg accggctcgg tccgctacgg ccagatcgag
     9301 atcgaatgcg gcggccagat ctcgggtgac gtccaggcgc agcccatggc cgagagcaat
     9361 gaggcgccgg ccagagccgc cgagcctgca tcagcggcgc gccctaacgt cgtgccattg
     9421 gcggacgacg cccggcacta agcaacgggc gcttcgctca cggagcaggg cgcgatgagg
     9481 tcgcagaacg cggccacagt tttcggcggg aggatcgcga gcgaatgcga tcgctcccgg
     9541 ggcgcttttg ctttggtggt gttttgcggc atggcggcac tcaccgcttg ctgcggacgt
     9601 ccgacaccga cgtttacgat gtcgccctcg tcggatgttg gcgccacggc cgcggccgtc
     9661 gtgccgccgg ccggcttgcc tgcgccacct ccgcctgcaa tgcccgcgga cttgacgggc
     9721 atgggcgctg ccgaggtgct ggcgctcctc ggctcacccg atttccgccg cagcgagcct
     9781 cccgcagagc tctggcagta ccgcgccgca gactgcatcc tcgacgtctt tctctatcga
     9841 gaccctggtg ggtatcgcgt cgtgcacgcc gagacacggc gacgccggca aatcgttgcc
     9901 acgggcaacg gatgcagcgc cggccccgcc ggcttcgtca gccatggccg gcagagccgg
     9961 ctctagcaac cagatggcgc aggcgttcca gaagctgtga ttcctgcata cgctggcacc
    10021 ggcgccgccg gaaccgccat tcgccgtcgc gcagctcgtc gtcctggcga tcttcgtcga
    10081 tacggccatt tcgccatgtc gccggagagg gcgatttcct gcgaatccgc ggctgaatgg
    10141 tagcgacaac gtccatacgg gctcgcttat tccatcatta aatcggcgaa agaatgggcg
    10201 atggcgtcgg ccttttcctc atatcacggt ccgagcgcca attgcgcccg acaggagaac
    10261 gccgatgaaa cgcataatcg ccctggcttt gctcaccctt cccctcctct gcagcggctg
    10321 catcatctat gatcgcgacg gcggctacca tcacgggtat tactggcgct gaccggggcc
    10381 gcctcgaccg acgcagaagc agaaaatgag gcgcgcatgg cgaagaaccg gctctggcgc
    10441 cgcaatcttg cgccaacgag gctgggtgcg ctcgccgcag cctgcgggtc gccgggctta
    10501 tgcgcgtctt cggcgggcgg tggcgcggac gccgcgtttc ttggcggcgg tcttgcgcac
    10561 cggcgtcggc acgctcgagg tcttatcggg gaaagcgcag agatcgttga cgacgcaagc
    10621 ctcgcatcgg ggcttccgcg cgatgcagac gtagcgacca tgcaggatga gccagtgatg
    10681 cgcgttgtgc ttccagcgat cgggcacgac acgctcgagc tccacttcga ccagcagcgg
    10741 cgtggcgcct ggcgccatgc cggtgcggtt ggcgacgcgg aagacatgcg tgtcgacagc
    10801 gatcgtattc tggccgaagg cgacgttgag cacgacgttg gcggtcttgc ggcccacacc
    10861 cggcaacgcc tcgagcgctt cccacgtccg cggtacctcg ccgccatcct cggcgatcag
    10921 cttctcgctc aaggcgatca cattcttggc cttggtgttg aagaggccga tggtcttgat
    10981 gaagtcgcgc agccgcgcct cgcccaaggc caccatcttc tgtggcgtat cggcgacttt
    11041 gaaaagcttc tccgtcgctt tgttgacgcc cgcatcggtg gcctgcgcgg acagcacgac
    11101 cgcgaccagc agcgtgtagg gattgatata gtcgagctcg ctccgaggat cgggccgctc
    11161 cttggcgagg cggcggaaga actcgtcgac gtcctgcgga gtcatacgac catcctaggg
    11221 caccgcgccg atcgggcgga agggcgttta cctcgggaag cggctgactt atggtgatgc
    11281 gatgagcgag ccccgcccgg cgaactcgcc acccgtactc tatgacgcgg aacttgcgcc
    11341 gcattgcagc ctgccgcccc gcggttttgc cgtgttgatg ctggtgctcg ccggcgcaag
    11401 cggcaccgtc ggcatggcct tcgtcctgcg cggcgcctgg cccgtgacgc cattcttcgg
    11461 cctcgacctg ctgctcgtct atctcgcctt tcgcatgagc tatcgccagg cgcgacgtcg
    11521 cgaggcgctg cggctgaccg aggagaactt gaccgtcgag cgcaccgatc cgaagggcgg
    11581 gcgtcgccac tggcacttcc accccttctg gctgcgcgtc gtcctcgaag agaaggacga
    11641 gcacagcaac cgcttggtgc tcgcatccca cggtcgctcg ctggtggtgg gaagcttctt
    11701 gggaccagac gagcgccgcg atgttgcccg cgacctgacg gatgcgctcg ggcgctggaa
    11761 gacgcggctc ccggccgacc gcgacgcgta gaactcactt cagtccgagc acatccttca
    11821 tcccatagag gccgggtttc tgcgccacga cccagcgcgc ggcgcggagc gcgcccagcg
    11881 cgaagatgcg gcggctcgtg gcgcggtgcg cgagttcgag gcgctcgcct tcgccggcga
    11941 agatcacgac gtggtccccc accacatcgc cgccgcgcag tgtggcaaag ccgatatcgc
    12001 cgcgcgtccg cgcgccggtg tggccgtcgc gcacacgttg actccgggcg ccaagatcga
    12061 tgccgcgtcc cgccgcagcg gcgcggccca gggcaagcgc cgtccccgac ggggcatcga
    12121 ctttatgatg gtgatgcatc tcgagcactt cgatgtcgta gccatcgtcg agtcgtcgcg
    12181 ccgcctcctc gaccagcgac aggagcatgg tgacggccgg gctcatattg ggcgcccaga
    12241 cgatcggcac gtgacgcgcc gccgtctcga tggttttcgc ctgcttcgca tcgagtcccg
    12301 tggtgccgat gaccagcgcc ttgccgcttt tcgccgcctg ctcggcatgg gttgccgaag
    12361 ctgccgggac cgtgaaatcg atcaccgcgt ccgaagccgc aaaaagcgcg gccagatccg
    12421 ctgttgccgc gatgccgact ggcccgatgc cagcgagctc gccgatgtcg cggccgagcg
    12481 acgaactccc gggggcttcg ctgccgccgg cgagccgcag accggccacg gcttgcgtct
    12541 ccgcgacgag catccgcccc atacggcccg cgcagcccgt gatgccgatg cgaatctcag
    12601 ccatgggata gcatctcctc gctcacgtct tttcgccgag agcctcgcat cggagtcggc
    12661 gaatgacaag cggctgcgac cgcgcgccat ggcgcccggc ggtgaattcc tctacatcct
    12721 ccgccgcatg gccttgctgc gctctgtcgc cacggtcggc ggctacacca tgatcagccg
    12781 cgtcctgggc ttcctgcgcg agatgctcac ggcggcattt ctcggcgccg ggcccttggc
    12841 cgacgcgttc ttcgtggcgc ttcgcctccc caatatgttc cgcagcctct tcgccgaagg
    12901 cgccttcagc gccgccttcg taccgctctt cgcggggagg ctcgcgcagg aaggacgtcc
    12961 ggcggcgcgg cgcttcgccg aggatacgct ggccgtgctt ctggtggcgc ttctcctgtt
    13021 cctggtcgcc ggcgagatcc tgacgccatg gatcctcgac ctgctggcac cgggcttccg
    13081 ggccgatccc gagaagttcg cgcgggccgt cgatctcacg cgcatcatgt tcccctacct
    13141 gctcttcatc tcgctgacgg cgctcgaggg cggcgtgctc aactcgctcg agcgcttcgc
    13201 ggcggcggcc gcgacgccgg tgctg
//
LOCUS       DASYNS010000085         5438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3454391, whole genome shotgun sequence.
ACCESSION   DASYNS010000085 DASYNS010000000
VERSION     DASYNS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454391"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VGP50_02645"
     CDS             complement(<1..878)
                     /locus_tag="VGP50_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEV8026298.1"
                     /translation="MSSSPIERPAAAWPRQPAAPGDSHRRWFKRVLPRSLFGRSLIII
                     VLPLVLAQLIATWIFYDRHWDTVQRRFATSVAGDVALMLDTMRYVDRSELPSLFAQAS
                     ASTELYYMFRPDEALPVDARETGLSQIEEQLGAALNERVRLPFLIDDDFDPRDVLISI
                     ELQDGVLQVAAPRKRLFTPTTYIFVLWMAGSSLVLLAIASIFMRNQVRALRRLAAAAE
                     RFGKGRDAPDFRLEGATEVRQAANAFLKMRDRIRRQITQRTEMLAGVSHDLRTPLTRM
                     KLALELMGEDPTVAEL"
     gene            complement(859..1545)
                     /locus_tag="VGP50_02650"
     CDS             complement(859..1545)
                     /locus_tag="VGP50_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8026299.1"
                     /translation="MSDTAESHLLVVDDDAPLRELLRRYLTEQGFRVTTAADAAEARA
                     KLQSLSFDLLVLDVMMPGEGGLDLTRALRRESRIPILLLTAMAEPEDRINGLERGADD
                     YIGKPFEPRELVLRIRNILQRVPQPSAVASEFRFGAFRLDLGRGELYRGSELVHLTAA
                     EVALLLALARRAGEAVSREALSEEAQFSGNVRTVDVQVTRLRRKIERDPKFPRYLQTV
                     RGTGYVLKPD"
     gene            complement(1640..2134)
                     /locus_tag="VGP50_02655"
     CDS             complement(1640..2134)
                     /locus_tag="VGP50_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV8026300.1"
                     /translation="MADVKSAANLLFLREDELRQGIELLYYAYRDFTAEPDAMLARYG
                     FGRAHHRVIYFVGRHPQMTVTELLGILRITKQSLSRVLGQLVRQGFIRQRTGSRDRRQ
                     RLLELTDKGVELERQLSENQRQRIAKAYREAGAAAVEGFRKVMLGIMAGEDDRRRFER
                     NAAR"
     gene            2224..3249
                     /locus_tag="VGP50_02660"
     CDS             2224..3249
                     /locus_tag="VGP50_02660"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV8026301.1"
                     /translation="MDMDTTISWKELGFRYLDTGVFVRADFRRGVWSAPEICAGNSMN
                     IHIAATCFHYGQACFEGLKAFRQTDGRVACFRADAHARRMIHSAERLVMVPPPESLFL
                     EAVQLVVRHSDAYVPPFGTGATFYVRPLLIGTSAVIGVHPSDDYTFIVLGMPVGPYYK
                     DGMVPVKAYVQEKYDRAAPHGVGHIKAAGNYAAALLGDREVKQQGYPVSLFLDSATHT
                     LIDEFGTSNFIGITRDGRYVTPKSESILESVTNDSLRKIAKDLQLRVEHRPIRWAEIE
                     DFVEIGACGTAAVITPIASITRGEQTLRFGAEDKPGKTLQRLYDEIQGIQYGKIPDRH
                     KWMMPVR"
     gene            3389..4162
                     /locus_tag="VGP50_02665"
     CDS             3389..4162
                     /locus_tag="VGP50_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEV8026302.1"
                     /translation="MAVRVEKDGPVTTVIIDRPEARNAVNPAMAHALADAFAAFDADD
                     AARVAVFWGAHGNFCAGFDLKVAAAGGSGATADGIGPMGPTRMRLAKPVIAAVAGYAV
                     AGGLELALWCDLRVAEDDAVFGVFCRRWGVPLVDGGTVRLPRIVGHGRALDMILTGRG
                     VGAAEALQMGLANRVVPKGAARAAAEALARELAALPQICLRQDRLSAYEQWDLEHGEA
                     MRNEFRRGSAVLASGEAAAGAGHFARGAGRRGAPAPRGD"
     gene            complement(4173..>5438)
                     /locus_tag="VGP50_02670"
     CDS             complement(4173..>5438)
                     /locus_tag="VGP50_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026303.1"
                     /translation="PATLCLVAITLLALSRTRRAQTLLAQANEEAARRERLEASLYQA
                     QKMEAVGQLTGGVAHDFNNLLTAIMGSLEMIIRAGDGTGAPVRKYAAAAMRAAERGAR
                     LTQQLLAFSRRQMLRPEAVNLNRLLRDFEELMQRAVGESIDFALELDPGLARCRVDPA
                     QFQSAMLNLVMNARDATQAGGRITIATANTVAGPGREPLDVELAPGGYVTATVRDTGS
                     GMAPEVRAHAFDPFFTTKEVGKGSGLGLSQVYGFARQSGGDVSVESEPGRGTSVRLYL
                     PSLGMPEEARAALAEEKEPREGPAKPARVLVVEDDDAVLEAARTVLSDLGYATLAARN
                     GAEALAILRRDEPIDLLFTDIVMPGGMNGITLAKEARQLRQDIKVLLTSGYDATVAER
                     QGADSFEILPKPYRQSQLADKLAGVLGGR"
BASE COUNT          854 a         1795 c         1944 g          845 t
ORIGIN      
        1 ttgagctcgg cgacggtcgg gtcctcgccc atcaattcga gcgccaattt catccgcgtc
       61 agcggtgtgc gcagatcgtg cgagacgccg gccagcatct cggtgcgctg ggtgatctgg
      121 cggcggatgc ggtcgcgcat cttgaggaag gcattggctg cctggcgcac ctcggtcgcg
      181 ccctcgaggc ggaaatcggg cgcgtcgcgt cccttaccga agcgctcggc cgcggccgcg
      241 agccggcgca acgcgcgcac ctgattgcgc atgaagatcg aggcgatggc gagcagcacc
      301 agcgacgagc cggccatcca cagcacgaag atgtaggtcg tgggcgtgaa gaggcgcttg
      361 cgcggcgccg ccacctgcag cacgccgtcc tgcagctcga tcgagatgag gacgtcgcgc
      421 gggtcgaaat cgtcgtcgat gaggaagggg agccgcacgc gctcgttgag cgccgccccg
      481 agctgctcct cgatctggct caagcccgtc tcgcgcgcgt cgaccggcag cgcctcgtcg
      541 gggcggaaca tgtaatagag ctcggtgctc gcgctcgcct gggcgaagag cgaggggagc
      601 tcgctgcggt cgacatagcg catggtgtcg agcatgagcg cgacgtcgcc cgcgacgctg
      661 gtggcaaagc gccgctgcac cgtgtcccag tgacggtcgt agaagatcca ggtggcgatg
      721 agctgcgcca gcaccagcgg cagcacgatg atgatgagcg agcggccgaa gagcgagcgc
      781 ggcagcacgc gcttgaacca gcggcgatgg ctgtcgcccg gcgccgccgg ctggcgcggc
      841 cacgccgccg ccggccgctc aatcgggctt gaggacatag ccggtgccgc gcacggtttg
      901 cagatagcgc gggaatttgg gatcgcgctc gatcttgcgg cgcagccgcg tcacctggac
      961 gtcgacggtg cgcacattgc cgctgaactg cgcctcctcg gagagcgcct cgcgcgacac
     1021 ggcctcgccg gcgcggcgcg ccagcgccag aagcagcgcc acctcggcgg cggtcagatg
     1081 cacgagctcg ctgccgcgat agagctcgcc gcgtcccaga tcgaggcgga aggcgccgaa
     1141 gcggaactcg ctggcgacgg cgctcggctg cggcacgcgc tgcaggatgt tgcggatgcg
     1201 cagcaccagc tcgcgcggct cgaagggctt gccgatgtag tcgtcggcgc cgcgctcaag
     1261 gccgttgatg cgatcctcgg gctcggccat cgccgtcagc aagaggatcg ggatgcggct
     1321 ctcccggcgc agcgcgcgcg tgagatcgag gccaccctcg cccggcatca tgacgtcgag
     1381 gaccaggaga tcgaaagaga ggctctgcag cttggcgcga gcctcggcgg cgtcggcggc
     1441 ggtcgtcacg cgaaagccct gttcggtgag atagcgccgc agcagctcgc gcaagggcgc
     1501 gtcgtcgtcg accaccagga gatgggactc ggcagtgtcg ctcatcggcg gcagtatacg
     1561 tcgcggcggg cgctcgcgtc atggtggcgt gatgtcgggt cggaggaaac atcatgccgg
     1621 cgcgtatcat ggcgtgcgcc taccgcgcgg cgttgcgctc gaagcggcgg cggtcgtcct
     1681 cgccggccat gatgccgagc atcaccttgc ggaagccctc gaccgcggcg gcgccggcct
     1741 cgcgataggc cttggcgatg cgctggcgct gattctcgga gagctggcgc tcgagctcga
     1801 cgcctttgtc ggtgagctcg aggaggcgct ggcggcggtc gcggctgccg gtgcgctggc
     1861 gaatgaagcc ctggcgcacg agctggccca gaacgcggct caagctctgc ttggtgatac
     1921 gcaggatgcc cagaagctcc gtcaccgtca tctgcgggtg acggccgacg aaatagatca
     1981 cgcgatgatg ggcgcggccg aagccgtaac gcgccagcat ggcatcgggc tcggcggtga
     2041 agtcgcgata ggcgtagtac aggagctcga tgccctggcg cagctcgtcc tcgcgaagaa
     2101 acagcaaatt tgcggcagat tttacgtcag ccatgttgac ttgttttcgt tgaaccggga
     2161 gggccgaagg tccatagtgg agcataatgc cggccaaggc accagggacg ccagcggaag
     2221 acgatcgaca tggatacgac aatctcgtgg aaagaactgg gttttcgcta tctcgacacg
     2281 ggcgtcttcg tgcgcgccga ttttcgccgc ggcgtctggt cggcgccgga gatctgcgcc
     2341 ggaaacagca tgaacatcca tatcgcggca acctgctttc actatgggca ggcctgcttc
     2401 gagggtctga aggcgttccg ccagacggat ggccgcgtcg cctgcttccg cgccgacgct
     2461 catgcgcggc gcatgatcca ctcggccgag cgcctcgtca tggtgccgcc gcccgagagc
     2521 ctcttcctcg aagcggtgca gctggtcgtc cggcatagcg acgcctatgt gccgcctttc
     2581 ggcaccggcg ccaccttcta tgtgcgtccc ctgctcatcg gcaccagtgc cgtgatcggc
     2641 gtccacccca gcgacgacta caccttcatc gtcctcggca tgcccgtcgg cccctattac
     2701 aaggacggaa tggtgccggt gaaggcctat gtccaggaga aatacgaccg cgccgcgccg
     2761 catggcgtgg gccacatcaa ggccgccggc aattatgcgg ccgcgctcct cggcgaccgc
     2821 gaggtgaagc agcagggata cccggtgtcg ctgttcctcg attccgcgac ccataccctg
     2881 atcgacgagt tcggcacctc gaacttcatc ggcatcaccc gcgacggccg ctacgtcacg
     2941 ccgaaatcgg agtcgatcct cgagagcgtg accaacgaca gcctgaggaa gatcgcgaaa
     3001 gacctgcagc tgcgggtcga gcatcggccg atccgctggg cggagatcga ggacttcgtc
     3061 gagatcggcg cctgcggcac cgccgccgtc atcaccccca tcgcttcgat cacgcgcggc
     3121 gagcagacgc tgcgcttcgg cgccgaggac aagccgggca agaccttgca gcgcctctat
     3181 gacgagatcc agggaatcca atacggcaag atcccggacc ggcacaagtg gatgatgccg
     3241 gtgcgctgac gctccgtagt aagattcacc acagagacac agaggcacgg agaagaggag
     3301 agtggcgcgc atcgtgccag gctttccttt ctatcttctt ctctgtgcct ctgtgtctct
     3361 gtggtgaatc tccatgtcgg aggggacgat ggcggtcagg gtcgagaagg acgggccggt
     3421 cacgacggtg atcatcgatc gcccggaggc gcgcaacgcc gtcaatccgg cgatggccca
     3481 cgcgctggcc gatgccttcg cggcgttcga tgccgacgac gcggcgcggg tcgccgtgtt
     3541 ctggggcgcg catggcaatt tctgcgccgg cttcgacctc aaagtggcgg cagccggagg
     3601 cagcggcgcg accgcggacg gcatcggacc gatggggccg acgcggatgc ggctcgcgaa
     3661 gccggtgatc gcggcagtcg cgggctacgc cgtcgcgggc gggctcgagc ttgcgctgtg
     3721 gtgcgatctg cgtgtcgccg aagacgacgc ggtcttcggc gtgttctgcc ggcgctgggg
     3781 cgtgccgctc gtcgatggcg gcacggtgcg cctgccgcgc atcgtcggcc atggccgcgc
     3841 cctcgacatg atcctcaccg ggcgcggcgt cggcgcggcc gaggcattgc agatgggcct
     3901 cgccaaccgc gtcgtgccga aaggcgcggc ccgcgccgcc gccgaggcat tggcgcggga
     3961 gctggcggcc ttgccgcaga tctgcctgcg ccaggaccgg ctctcggcgt atgagcaatg
     4021 ggacctcgag cacggcgaag cgatgcgcaa cgagtttcgc cgcggcagcg ccgtgctcgc
     4081 gagcggcgag gcggcggccg gcgccggaca tttcgcgcgc ggcgccggac ggcgcggcgc
     4141 accggcaccg cggggcgact gagagcaggg gatcagcgcc cgccgaggac gccggcgagc
     4201 ttgtcggcga gctgcgactg gcgatagggc ttgggcagga tctcgaaact gtcggcgcct
     4261 tgccgctcgg cgacggtggc gtcgtagccc gaggtcagca gcaccttgat gtcctggcgc
     4321 agctggcgcg cctctttcgc caacgtgatg ccgttcatgc cgcccggcat gacgatgtcg
     4381 gtgaagagca ggtcgatggg ctcgtcgcgc cgcaggatcg ccagcgcctc ggcgccgtta
     4441 cgcgcggcga gcgtcgcata gccgaggtcg gagagcacgg tccgcgccgc ctcgagcacg
     4501 gcgtcgtcat cctcgaccac gagcacgcgg gcgggcttcg ccggtccttc gcgcggctcc
     4561 ttctcctcgg cgagcgcggc ccgcgcctct tccggcatcc cgagcgacgg cagatagagg
     4621 cgcacgctgg tgccgcggcc gggctcgctc tccacgctca catcgccgcc cgattgcctg
     4681 gcgaagccgt agacctgcga gaggccgagc cccgagccct tgccgacctc cttggtggtg
     4741 aagaaaggat cgaaggcatg ggcccgcact tcgggtgcca tgccgctgcc ggtgtcgcgc
     4801 accgtcgccg tcacatagcc gccgggcgcg agctcgacgt cgagcggctc gcggccgggg
     4861 ccggcgacgg tgttcgccgt cgcgatggtg atgcggccgc ccgcttgcgt cgcgtcgcgg
     4921 gcattcatca cgaggttcaa catggccgac tggaattgcg cgggatcgac gcgacagcgg
     4981 gcgaggccgg gatcgagttc gagcgcgaag tcgatgctct cgccgacggc gcgctgcatc
     5041 agctcctcga agtcgcgcag cagccggttc aggttgacgg cctcgggccg cagcatctgc
     5101 cggcgcgaaa aagcgaggag ctgctgcgtg agccgcgcgc cgcgctcggc ggcgcgcatc
     5161 gccgccgccg cgtatttgcg cacgggcgca ccggtgccgt cgccggcgcg gatgatcatc
     5221 tcgaggcttc ccatgatcgc cgtcagcaga ttgttgaagt catgggcgac gccgccggtg
     5281 agctggccga ccgcttccat cttctgcgcc tggtagagcg acgcctcgag gcgctcgcgc
     5341 cgcgccgcct cttcattcgc ctgagccagc aaggtctggg cacgccgggt gcggctcaag
     5401 gccagcaacg tgatggcgac gaggcagagc gtggctgg
//
LOCUS       DASYNS010000086         4909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2363041, whole genome shotgun sequence.
ACCESSION   DASYNS010000086 DASYNS010000000
VERSION     DASYNS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363041"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VGP50_02675"
     CDS             <1..824
                     /locus_tag="VGP50_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026304.1"
                     /translation="VDPDIAEPVQLMQADGLKPIVASTDGVLVGEVRAAERRVWILSD
                     PDVMANHGIGRNDNAAFAVALIQALRGAEGAIVFEDTTHGLLGRATKAARPPLPSNPL
                     KLPFDARFLPATLQGGLAIVLLLWAAVMRFGAPVPAPPPLDAGKRGLIQNAARLIAFA
                     GYQRVIAKRYVEATIREVGRQIHAPRGLADSGLVDWLGRVGEARGVDRDCALVYRQTR
                     TLDQGRGRGGDLSSVMPLLRDIYRWKQEMIDGPSRNPRDHRGRAPRGAQSGDRPG"
     gene            748..1704
                     /locus_tag="VGP50_02680"
     CDS             748..1704
                     /locus_tag="VGP50_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV8026305.1"
                     /translation="MDLQEIRAITEGVRREVRKAVIGQDEAIELMLTSLLVGGHVLLE
                     GVPGTAKTLLTQAFAAGLALQFGRIQFTPDLMPGDVLGTNLFNFQTNSFVLTKGPIFT
                     QLLLADEINRTPPKTQAALLQAMNERAVTVDGHDYALGDAFMVVATQNPIEQQGTYPL
                     PEAQLDRFLFKLVLHHPARDEEREIVRRHGHRSAMPRLSDFGLEAIADADALSRTREA
                     VARIRLSDALVDYIVDVVRATRQHSSLEVGASTRAANMLAAAVRAFAALQGRDFVIPD
                     DVKALALPVLRHRVSLSPSAEIEGLATDRILGEILEQVTAPR"
     gene            1701..3014
                     /locus_tag="VGP50_02685"
     CDS             1701..3014
                     /locus_tag="VGP50_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV8026306.1"
                     /translation="MIRPSLRAVLIFAAGLPGALFLVIYDAALWPFSFDYGALALLAI
                     ATDALLSLPARALRLDIAVPDRLFIGETGAITVTLPAIPYRRPLRVELLAELRGELDP
                     AQIVKTELAVGAAGRAELPLVPRRRGKVAVDAVWARWRGPFKLVRRTQRIKLERSVDV
                     LPNIRGVQRAALQFFAREAIFGVKVQTQRGEGAEFESLHEYVPGLDSRFIDWKASARH
                     RKLVCKEFKTERNHQIMLAFDTGYLMREPIEGIPRLDHAINAGLLLAWISLQGGDLVG
                     TYGFDAAVRHYRGPVRGIANFSRVQQAAAELAYRQEETNFTLGLAGLSARLKRRALIV
                     LFTDFVDTVTAELLIESVERMASRHVVVFVTLRDSLLQKTVDAAPADFERVAEAVIAH
                     DFLRDRSIVFERLDRMGVHCLDVPSRGLSVNLINRYLLIKQRGLI"
     gene            3011..4063
                     /locus_tag="VGP50_02690"
     CDS             3011..4063
                     /locus_tag="VGP50_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEV8026307.1"
                     /translation="MTEAVTLHQSAAASPASAEPTLKSSEFRKGRESSWRDLEGLVER
                     VEKRGIAALLPDELQRIPLLYRATLSSLSVARSIALDRNLLLYLENLSLRAFLVVYGP
                     RTSLVEGCAEFLRRGFPRAVRAARWHFLLAIAALAVGAMAGFMLTAGDERWFSALVPG
                     GLGGDRGPSSTRAELLDHEIFAPWPGAAAAFGLFASFLFSHNTLIGILTFGLGLAAGV
                     PTLLLLLYQGLTLGAFVALHYHRDLTVDFIGWISIHGVTELSAIALCGAGGFVIADTI
                     LFPGRHSRLDSLALRGRMAAEIAIGAAAMFFVAAILEGGFRQLVQSTELRFAIAGVTA
                     IAWLAYFGFAAREPRR"
     gene            4060..>4909
                     /locus_tag="VGP50_02695"
     CDS             4060..>4909
                     /locus_tag="VGP50_02695"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEV8026308.1"
                     /translation="MTAAYEQASLFLEGGSRNRREIITPEGVPLPVEIATFGERATAF
                     VLDFFIQFCATVLVYLFVIFVAFGSFLAFTKSGGAIILAILLLLAFLIRNVYFIHFEL
                     AWQGATPGKRALGLRVIDRRGGPLLPTAVIARNLTREMEMFLPLGILLSAGRGPVWEE
                     LSLAAWLIAFALLPLFNRDRLRGGDLIGGTMVVVLPRRVLLGDLVGQSSRYRFTDRQL
                     DAYGAFELQVLEELLRRPNAPDAARLHREVCDKICRKIDWRTPVPEADTLAFLKDFYT
                     AERAYLE"
BASE COUNT          684 a         1777 c         1569 g          879 t
ORIGIN      
        1 gcgtcgatcc cgatatcgcc gagccggtac agctcatgca agccgacggc ctcaagccca
       61 tcgttgccag caccgacggc gtcctcgtcg gcgaggttcg cgccgccgag cgacgcgtct
      121 ggatcctgag cgatcccgat gtcatggcga accacggtat cgggcggaac gacaacgccg
      181 ccttcgcggt ggcgctcatc caggcgctcc gcggtgccga aggcgccatc gtcttcgagg
      241 acacgacgca tggcctcctg ggccgcgcta ccaaggcggc gcggccgccc ctcccctcca
      301 acccgctgaa gctgcccttc gatgcccgct ttctgccggc gacgcttcag ggcggcctgg
      361 cgatcgtgct gctgctctgg gcagccgtca tgcggttcgg cgcgcctgtg cccgcgccgc
      421 cgccgctcga tgccggcaag cgcgggctga tccagaacgc cgcgcggctg atcgccttcg
      481 ccggctatca gcgggtgatc gccaaacgct atgtcgaggc gacgatccgc gaagtggggc
      541 ggcagatcca cgcgccccgc gggctcgccg acagcggcct cgtcgattgg ctcggccgcg
      601 tcggcgaggc gcggggcgtc gatcgcgact gcgcccttgt ctatcgccag acccggactc
      661 tcgaccaagg ccgcggacgc ggcggcgacc tctcgtccgt catgccgctg ctgcgcgaca
      721 tttaccgctg gaaacaggag atgatcgatg gaccttcaag aaatccgcgc gatcaccgag
      781 ggcgtgcgcc gcgaggtgcg caaagcggtg atcggccagg atgaggccat cgagctgatg
      841 ctgacgagcc tgctcgttgg cgggcatgtt ctgctcgagg gcgtgcccgg caccgccaag
      901 acgctcctca cccaagcctt cgccgccggt ctcgccctcc aatttggacg catccagttc
      961 acgcccgatc tgatgccggg cgacgtgctc ggcaccaatc tcttcaattt ccagaccaac
     1021 agcttcgtgc tgaccaaggg gccgatcttc acgcagctcc tgctcgccga cgagatcaac
     1081 cgcaccccgc ccaagaccca ggcggccttg ctccaggcga tgaacgagcg ggccgtcacc
     1141 gtcgacgggc acgactatgc gctgggcgat gcgttcatgg tggtggcgac gcagaacccg
     1201 attgagcagc aggggactta tccgctgccg gaagcgcagc tcgaccgctt cctcttcaag
     1261 ctcgtcctcc accacccggc gcgcgacgag gagcgcgaga tcgtgcgccg ccacggccat
     1321 cgctccgcga tgccgcgcct cagcgatttc gggctcgagg cgatcgccga cgccgacgcc
     1381 ttgtcgcgca cccgcgaagc cgtggcgcgc atccgcctct cggacgcgct cgtcgactat
     1441 atcgtcgacg tcgtgcgcgc cacccgccag cattcctcgc tcgaggtcgg cgcgtcgacg
     1501 cgcgccgcca acatgctcgc cgcggcggtg cgggccttcg ccgcgttgca gggacgcgat
     1561 ttcgtcattc ccgacgacgt caaggcgctg gcgttgccgg tgcttcgcca ccgcgtcagc
     1621 ttgtcgccga gcgccgagat cgaaggactg gcgaccgatc ggatcctcgg cgagatcctc
     1681 gagcaggtga cggcgcccag atgatcaggc cgagcctccg cgccgtgctg atcttcgccg
     1741 cgggcctgcc cggggcgctt tttcttgtga tctacgacgc ggcgctctgg cctttttcct
     1801 tcgactatgg cgcgctcgcg ctgctggcga tcgccaccga tgcgctcttg tcgctgccgg
     1861 cgcgcgcttt gcgcctcgac atcgccgtac ccgatcggct cttcatcggc gagacgggag
     1921 cgatcaccgt cacccttccc gcgataccct atcgccgccc gctccgcgtc gagcttctgg
     1981 ccgaattgcg cggcgaactc gatccggccc agatcgtcaa gacggagctc gcggtgggcg
     2041 ccgcgggccg cgccgagctt ccgctcgttc cgcgccgccg cggcaaagta gcggtcgacg
     2101 ccgtctgggc gcgctggcgg gggccgttca agctcgtgcg gcggacgcag cggatcaagc
     2161 tcgagcgatc ggtcgacgtc ctgcccaaca tccgcggcgt gcagcgcgcc gctctgcagt
     2221 tcttcgcgcg cgaggccatc ttcggcgtca aggtccagac gcaacgcggc gaaggcgccg
     2281 aattcgaatc gctgcacgag tatgtgcccg ggctcgacag ccgcttcatc gactggaagg
     2341 cgtcggcgcg ccaccgcaag ctcgtctgca aggaattcaa gaccgagcgc aatcaccaga
     2401 tcatgctggc cttcgacacc ggctatctca tgcgcgagcc gatcgagggc attccgcgcc
     2461 tcgatcacgc catcaacgcc ggcctgctgc tggcctggat ctcgctccag ggcggcgatc
     2521 tcgtcggcac ctatggcttc gacgcggcgg tccggcacta ccggggcccc gtccgcggca
     2581 tcgccaattt ctcgcgcgtg cagcaggcgg cagcggagct cgcctaccgc caagaggaga
     2641 cgaacttcac cttgggtctc gccggcctga gcgccaggct caagcgccgc gcgctcattg
     2701 tgctcttcac cgacttcgtc gacacggtga cggccgagtt gttgatcgag agcgtcgagc
     2761 gcatggcgag ccggcatgtc gtcgtgttcg tcacgctgcg cgattcgctg ctccagaaga
     2821 cggtcgacgc cgcacccgcc gatttcgagc gcgtcgccga agcggtgatc gcccacgact
     2881 tcctgcgcga ccgcagcatc gtcttcgagc ggctcgaccg catgggcgtc cattgcctcg
     2941 acgtcccgag ccgcggattg tcggtgaacc tcatcaaccg ctatcttctc atcaagcaac
     3001 gcgggctcat atgacagagg ccgtcacgct ccaccaatcc gccgccgcgt cgccggcgag
     3061 cgccgagccg acgctcaaga gcagcgaatt ccgcaagggc cgggagtcga gctggcgcga
     3121 cctcgaaggg ctcgtcgagc gcgtcgagaa gcgcggcatc gccgcgctgc tgcccgatga
     3181 attgcagcgc atccctttgc tctaccgcgc gactctctcc tcgctctcgg tggcgcgcag
     3241 catcgccctc gaccgcaacc tcctgctcta tctcgagaac ctctcgctgc gggcgttcct
     3301 ggtggtgtat gggccgcgga cgagcctcgt cgagggttgc gccgagtttc tgcgccgcgg
     3361 ctttccgcgc gccgtgcgcg cggcgcgctg gcatttcctt ctggccatcg ccgcgctggc
     3421 ggtcggcgcc atggcgggct tcatgctcac tgccggtgac gagcgctggt tttcggccct
     3481 cgtgcccggc ggcctcggcg gcgaccgggg gccatcgagc acgcgggccg agctcctcga
     3541 ccatgagatc tttgccccct ggcccggagc tgcggccgcg ttcgggctct tcgccagctt
     3601 cctcttctcg cataacacgc tcatcggcat cctcaccttt ggtctcggcc tcgccgccgg
     3661 cgtgccgacg ctgctgctgc tgctctacca gggcctgacg ctcggcgcct tcgtcgcgct
     3721 ccactatcac cgcgacctga cggtggattt catcggctgg atctcgatcc acggcgtcac
     3781 cgagctttcg gcgatcgcgc tctgcggcgc cggcggcttc gtgatcgccg acacgatcct
     3841 cttccccggg cgccattcgc gcctcgacag cctggcgttg cgcggccgga tggcggccga
     3901 gatcgccatc ggcgccgcgg cgatgttctt cgtggcggcg atcctcgaag gcggcttccg
     3961 gcagctggtg cagagcacgg agctgcgctt cgccatcgcc ggtgtgacgg cgatcgcgtg
     4021 gcttgcctat ttcggcttcg ccgctaggga accgaggcga tgaccgccgc ctatgagcag
     4081 gcctccctct tcctcgaggg cggcagccgc aaccgccgcg agatcatcac gccggaaggc
     4141 gtgccgctgc cggtcgagat tgccaccttc ggcgagcgcg cgacggcctt cgtgctcgat
     4201 ttctttatcc aattctgcgc cacggtgctg gtctacctct tcgtcatttt cgtcgccttc
     4261 ggcagcttcc tggccttcac caagagcggc ggtgcgatca tcctcgccat ccttctcctt
     4321 ctcgccttcc tgatccgcaa cgtctacttc atccatttcg agttggcttg gcagggcgcg
     4381 acgccgggga agcgcgcttt gggactgcgc gtcatcgatc gccgcggcgg gccgctgctg
     4441 ccgacggcgg tcatcgcccg gaacttgacg cgcgagatgg agatgttcct gccgctcggc
     4501 attctcttgt cggcgggccg cggacccgta tgggaggaat tgtcgctggc ggcctggctc
     4561 atcgccttcg ccctcctgcc gctcttcaac cgcgatcgct tgcgtggcgg cgatctgatc
     4621 ggcggcacca tggtcgtcgt tctgccgcgg cgcgtgctgc tcggcgatct cgtcgggcaa
     4681 tcgagccgct accgcttcac cgaccggcag ctcgacgctt atggcgcctt cgagctgcag
     4741 gttctggagg agttgctgcg ccggcccaac gcgccagatg cggcccggct gcaccgcgaa
     4801 gtctgcgaca agatctgccg caagatcgac tggcgcacgc ccgttccgga ggcggacacc
     4861 ctcgcctttc tcaaggactt ctacacggca gagcgcgcct atctcgagc
//
LOCUS       DASYNS010000087        14310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3766803, whole genome shotgun sequence.
ACCESSION   DASYNS010000087 DASYNS010000000
VERSION     DASYNS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 14310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14310
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3766803"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VGP50_02700"
     CDS             <1..228
                     /locus_tag="VGP50_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026309.1"
                     /translation="AEAAIAGFVDWTVPAADLESAAYDVAERLTKIDMPSHAATKRRA
                     REPAIKAIRATIDQDITLAYAEERVAQRNAA"
     gene            complement(249..668)
                     /locus_tag="VGP50_02705"
     CDS             complement(249..668)
                     /locus_tag="VGP50_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV8026310.1"
                     /translation="MTQRPRFFHLMSMAQHRLLKAIDAAYKEALGISSTQLGVLFILE
                     KKPGALLKEVSEELGINASAITALIGRMEDARLVRRQDSAEDGRAVHLFATAEGLAKA
                     TTARPILGRLNARLTQGFSERDVATVGRFLNAILERF"
     gene            complement(719..841)
                     /locus_tag="VGP50_02710"
     CDS             complement(719..841)
                     /locus_tag="VGP50_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026311.1"
                     /translation="MRPRRGNLNPDAGRLDWYSVHLAETLRDGVPVQVTRPVPY"
     gene            complement(964..1545)
                     /locus_tag="VGP50_02715"
     CDS             complement(964..1545)
                     /locus_tag="VGP50_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEV8026312.1"
                     /translation="MRLPLQITFRGMEASDAITARIRERAEELDRLSDRIVACHVVVD
                     SGQGHQRKGRIHHVRVTLTVPGGEIAVTRDPPEHNAHEDMNVAIRDAFDATRRQLEDH
                     LRRHHGQVKTHDLPDHGAVSRLFPGQGYGFITAPAGQEIYMHRNSVLNGGFDALRIGD
                     AVIYVVDPEDGGEGPQASSVVAVGKRGPAAPFS"
     gene            complement(1611..1766)
                     /locus_tag="VGP50_02720"
     CDS             complement(1611..1766)
                     /locus_tag="VGP50_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026313.1"
                     /translation="MTSSALHAFLATPHPRMPSFILEPQETKDVVALLRASIDATPLI
                     QVNAARP"
     gene            complement(1824..2264)
                     /locus_tag="VGP50_02725"
     CDS             complement(1824..2264)
                     /locus_tag="VGP50_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026314.1"
                     /translation="MQLREAILRETLNGLRDRAARYRRVSSLLGGRPKDSRLTALARE
                     IEEWADQIERTMEKVHTQVAATHVLVAEIDACIGRAGQEVAFSKMRRNGRYSADDLRE
                     EARLCAEEARAASDLAEKRKLAATAADLADAAESVERVEATPEV"
     gene            complement(2277..2492)
                     /locus_tag="VGP50_02730"
     CDS             complement(2277..2492)
                     /locus_tag="VGP50_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026315.1"
                     /translation="MSHDPTLPSLSSDEDIAQELERVAQMVRERPELNHHAADRLLRI
                     ALSIRTDAARRLAPVIGKAPSEAPKGS"
     gene            2580..3473
                     /locus_tag="VGP50_02735"
     CDS             2580..3473
                     /locus_tag="VGP50_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026316.1"
                     /translation="MIQVKPTGVCSDLASRPSFHSSRISRFEHRRTDHAGNTEAEVYG
                     AVDLPFVPPELRENRGEIEAAAAGRLPPLVELGDLRGDLHTQSTATDGHNAVAEMAEA
                     ARAAGLEYIAVTEHWRRIAMAPGLDLARLRDQIAEIDRLDAEGSGIALLKAIEVDILE
                     DGKLDLPDSMLGEFDLVAAAIHSRFNLTRERQTERILHAMDRPYFTILAHPIGRLIGE
                     RPPYDVDMPRVIAAAHARGCFLELNAHPERLELSEVHLPHGEGGGRARQHRHRRASRR
                     RVLLSALRDRRGAAGSGRTTC"
     gene            complement(3540..4235)
                     /locus_tag="VGP50_02740"
     CDS             complement(3540..4235)
                     /locus_tag="VGP50_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8026317.1"
                     /translation="MSAAVAATEPSSPGRPPLSALAGSARSEALAALDRIGKEISLGR
                     DEGLFLEGDAAQYCFKVVAGAVRSCRLLGDGRRHVAEFFLRGDFIGLAAEPAYPFTAE
                     AVTDATLLRFPRIAIEKMISLRPSLGKWQVELLCRDLSSAQQQMLLLGRKNAIERIAS
                     FLLLMADRGHDAEHVSLPMTRSDIADHLGLTTETVSRIFGQLRSQRVIELRGASHVLV
                     SDIDALEELAQAA"
     gene            complement(4420..5028)
                     /locus_tag="VGP50_02745"
     CDS             complement(4420..5028)
                     /locus_tag="VGP50_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV8026318.1"
                     /translation="MRQEFSRALEFRHACKLFDETKALARADLDYVLEAGRLSPSSLG
                     LEPWRFIVIEDRALRRRLRPACWNQTQITTASAMIVILALKAELAPGSGYAQKMLGRL
                     VSSPELLDEAMQIYHDIARGDVAAWSIAQCHIAAADMMLAAAVIGLDSCPMGGFEPEA
                     VAEVLGIDRTRVEVALLLALGHRVEPQPSKHRLPMADLVEYR"
     gene            complement(5119..5874)
                     /locus_tag="VGP50_02750"
     CDS             complement(5119..5874)
                     /locus_tag="VGP50_02750"
                     /inference="COORDINATES: protein motif:HMM:NF022187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV8026319.1"
                     /translation="MTEDSSCKPSRRAFLAATSLVVGAVAASPLAAFAQTAGGAPLKI
                     GTIGAGHEGGALGTLFAKAGHPVMFSSRNPESLKDLVADAGPTAQAGTVAQAVAFGDV
                     ILLAVPYPAVEEIGHDHGKAIASKQLVLDVSNPIPLRDGAAFVQGIADQGGPGVVTAK
                     LLPGAHIVRAFNAISYRSLTPNAHRTGEPLGIPIAGDEEKAIALATRLIKEIGFEPVL
                     VGGLAMGKYLVPGTPLGGEHTPAEIRQIAATLK"
     gene            complement(5971..7005)
                     /locus_tag="VGP50_02755"
     CDS             complement(5971..7005)
                     /locus_tag="VGP50_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV8026320.1"
                     /translation="MKYRVLGRTGLFVSEVCLGTMTFGGRGFWRAIGQLDQDATTALV
                     RAALDAGVNFLDTADVYSEGESERLLGRALKDIGANRADLVIATKARGRTGPGPNAVG
                     LSRGHIMTAVQESLKRLDTDYIDLYQIHGADIVTPIDETLRALDDLVRGGLVRYIGCS
                     NLMAWQVAKALGLSAARELARFETVQAYYSIASRDIEREIVPLLTAEALGLMVWSPLA
                     GGLLSGKFKREGAGPNDARRTSFDFPPVDRDRVFAIVDVMRPVAERHGASVARVALAW
                     LLHQKHVTSVIIGAKTIEQLQDNIAATELRLTEDELATLDKASALKPEYPRWMIDRQN
                     AERVPGASKR"
     gene            complement(7169..7615)
                     /locus_tag="VGP50_02760"
     CDS             complement(7169..7615)
                     /locus_tag="VGP50_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026321.1"
                     /translation="MTLAELIQVLEAAKNGSAGLDRLICETVATVDRRDDCFWAFDHA
                     ASGSDVMELDAPSRVHHYTTSIDAALELVPEGWSWNLRVLCRSDRATPPTIRAVVFNN
                     AIGMVLSSEYCEAEAETPPIGLCIAALRARANLQKPPIGQARRDVP"
     gene            8045..9736
                     /locus_tag="VGP50_02765"
     CDS             8045..9736
                     /locus_tag="VGP50_02765"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEV8026322.1"
                     /translation="MTVVLGVGTLLSLSCWLAAARWADDLDRAEFVRRATNHQLILQA
                     GFREYEADERWNVVTTPVEGDLRGAWRPSALTVLGGGLLAAALGALLARRRARRQAEL
                     ERLAANLRVTTARLVEQAEQRVSYLAGHDVVTGLPNRTFFVARLAEHLEEFRRGASPF
                     AVHYLDLDHFKDVNDTLGHDAGDELLKEIGQRLLRAVRTTDVVARLGGDEFAVLQADT
                     KMPDDAAALAVKLRDLVTAPYILAGNHIVTTASIGIALSTGDTADAATMMKQADIALY
                     KAKGEGRDRFCFHSEELGKAVRARVALASELRRAVAEHEFFLEYQPQFALAGDELVGV
                     EALLRWRHPAKGVVAPDLFMPAAESSGLILPIGDWVLREALRQGRAWIDAGCAPTTLA
                     VNVSAIQIKASDFEARLLEALRDSGFPARCLELELTETVLFETTKVHEQILVRLHDAG
                     IRVAIDDFGTGFSSLAYLMRFHTDRLKIAQTFVRHIATDEHAASIVKAAVSLAQALGL
                     SVVAEGVESPEQLEMLRRFGCDAVQGYLYAPPLSADGVADLVRARRSATLALAAE"
     gene            9813..9965
                     /locus_tag="VGP50_02770"
     CDS             9813..9965
                     /locus_tag="VGP50_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026323.1"
                     /translation="MRNDSLLQLAERANRLQVLLTVPTMIFIMPTIFLVVGGPTALKL
                     IDLLWC"
     gene            10169..10837
                     /locus_tag="VGP50_02775"
     CDS             10169..10837
                     /locus_tag="VGP50_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV8026324.1"
                     /translation="MTPVDAEPYSFDLDPAHAALLIIDMQRDFLEPGGFGEMLGNDVS
                     QLRRAIEPNRRLLEAWRAADLLVIHTREGHRPDLADLPLAKKERGRGDKTIGDRGPLG
                     RILVRGEPGHDIISELYPRAGEPVIDKPGKGAFFATDLHAILDIRAIRQLVVTGVTTE
                     VCVNTTVREANDRGFDCLVIEDCVASYFPEFQAAGLDMIKAQGGIFGWVAHSADVIRA
                     IGRR"
     gene            10881..12479
                     /locus_tag="VGP50_02780"
     CDS             10881..12479
                     /locus_tag="VGP50_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026325.1"
                     /translation="MASMAVAQAKPKLWVPGDWNAFFGFGTNILVNVLTLTALLRFVL
                     KMPDSLVFGRILPATGLMLCLSTLYYAWLAYRLAQKTGRSDVCALPSGTSVPHMFVVV
                     FVVMMPILGKTGDPVQAWEAGLTWVFVQSFVLMAGGFIAPVIRRITPRAALLGSLAGI
                     SITFISMRPASQMFVTPVIGLVCFALILVSWFGGVRYFKGVPAGLVAIGVGTIIAWGS
                     NFYGLHYGGLSVGALADAVANFGFSYPVPALAHVLGGFKFLGVILVTAIPFGIYDLVE
                     AMDNVESAAAAGDSFPTTRVLTADGVISLIGCLMGNPFINAVYIGHPGWKSMGGRIGY
                     SAATGIFVILLSWLGVVAVMMTLIPVVAILPILLYIGMLIGSQAFQETPKSHAPAIIL
                     ALVPQIAAWGKLQIDGALGAAGTNATALGFDKLEQVGVLYRGLEVLGGGATLAGIVLG
                     AVTVFVIESQFLKASAFALAGAVLTFFGLIHSEAIGIGSSPAVAVSYLGIAAFLFGCA
                     RFAKVVPQMAAMPHAHRLSTEPAE"
     gene            12496..12684
                     /locus_tag="VGP50_02785"
     CDS             12496..12684
                     /locus_tag="VGP50_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4089 domain-containing protein"
                     /protein_id="HEV8026326.1"
                     /translation="MGTTPFDAGTYVDAAAAAIDLPIAPEHRPGVILNLERLAAMAQL
                     VMAFPLAKETAPAPVFRA"
     gene            12681..14048
                     /locus_tag="VGP50_02790"
     CDS             12681..14048
                     /locus_tag="VGP50_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtzE family amidohydrolase"
                     /protein_id="HEV8026327.1"
                     /translation="MIGAGSDAVDIAAAVSAGRVSARAVTDAALDRIAAGNQRLNAFT
                     AVTAERARKAAAAIDAKRARGEALGLLAGVPYAVKNLFDIAGVTTLAGAKIEAGKAPA
                     AADAAAVRRLDAAGAVLVGALNMDEYAYGFTTENAHYGPTRNPHDVLRSAGGSSGGCG
                     AAVAGGLVPLSVGTDTNGSIRVPASLCGIFGLKPTYGRLDRAGAMAFVDSLDHVGPFA
                     RSVRDLALCYDALQGGTSAPVEPTVPRLGDGVAGLRVAVADGYFAPAGADEAQDAVAL
                     VARVLGATRRVELPESARARAAAFVISAAEGANLHLPDLRRRAADFDPATRDRFLAGA
                     LVPAAWLLQAQRFRRWYRAQVLEVFASTDVILAPATPCAAPRLGQETIMLEGKEIAVR
                     PNLGLFTQPLSFIGLPVVVVPVRRDGALPIGVQIIAAPWCEAMALRVAAALEDAGAVS
                     ARLAG"
     gene            14081..>14310
                     /locus_tag="VGP50_02795"
     CDS             14081..>14310
                     /locus_tag="VGP50_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtzH-like domain-containing protein"
                     /protein_id="HEV8026328.1"
                     /translation="MYEINIPQIVAEVTAAFARYEQALVTNDIDVLNELFWNSPHTLR
                     LGATENLYGYQAITGFRAARSPLNLARRLRRTV"
BASE COUNT         2235 a         4725 c         4801 g         2549 t
ORIGIN      
        1 gccgaagccg ctatcgccgg cttcgtcgac tggacggttc ccgcggccga tctggagtcc
       61 gccgcttacg acgtcgccga gcgcctgacc aagatcgaca tgccgtccca tgccgcgacg
      121 aagcggcgcg cgcgcgagcc cgccatcaag gcgatccgcg ccacgatcga tcaggacatc
      181 accctcgcct acgcggagga gagagtcgcc cagcgcaacg ctgcatgacc gtgcagcggc
      241 gggtcggctc agaagcgctc gagaatggcg ttgaggaatc gcccgaccgt cgcgacgtcg
      301 cgttcgctga agccctgcgt gaggcgggcg ttgagccggc ccaggatcgg ccgtgccgtc
      361 gtcgccttgg caaggccttc cgccgtcgca aagagatgca cggcccgtcc gtcttccgcc
      421 gaatcctgac gccgcaccag ccgggcgtcc tccatccgtc cgatcagcgc ggtgatggcc
      481 gacgcattga tgccgagctc ctcgctgacc tccttgagga gggcccccgg cttcttctcg
      541 agaatgaaca gcacgccgag ctgcgtcgag ctgatgccga gcgcctcctt gtaggcggcg
      601 tcgatggcct tgagcaagcg atgctgcgcc atcgacatca gatggaagaa acggggccgc
      661 tgcgtcatgc ccggacgtta gtcgaaaacc cttcacaggt gaagcgaagc gcgccggctc
      721 aataaggaac cggccgcgtc acctgtacgg gcacgccgtc gcgcagcgtt tccgccaggt
      781 gaacgctgta ccagtcgagc cgcccggcat cgggattaag attgcctcgc cggggtctca
      841 ttgacggatg gcggcatttc cgtcgacaac atcgccgttg aggagatctg ggacgccgtc
      901 gacgacggca cgcccgccga tctggccgtg acggcggcgc tatgacaacc cgtgccgcgc
      961 ggctcagcta aacggcgccg cgggtccgcg cttgccgacg gcgaccaccg aactcgcttg
     1021 cggtccctcc ccgccgtcct cgggatcgac gacgtagatg accgcgtcgc cgatgcgcaa
     1081 cgcgtcgaac ccgccattca gcacgctgtt gcgatgcatg tagatttcct gcccggccgg
     1141 cgcggtgatg aaaccgtagc cttgaccggg gaacagccgc gacaccgcgc cgtgatcggg
     1201 cagatcatgc gtcttgacct ggccatgatg gcgacgcagg tgatcctcga gctggcgacg
     1261 ggtggcgtcg aaggcgtcgc ggatggcaac gttcatgtcc tcatgggcgt tatgctccgg
     1321 tggatcgcgc gtcacggcga tctcgccgcc gggcacggtg agcgtcacgc gaacatgatg
     1381 gatgcggccc ttccgctggt gcccttggcc ggagtcgacg acgacgtggc acgcgacgat
     1441 ccgatccgag aggcggtcca gctcttcggc acgctcgcgg attctcgccg tgatcgcgtc
     1501 ggacgcttcc atgccgcgaa acgtgatctg cagcgggagg cgcatgtcat tctccttctc
     1561 gaggtctttg gcccggtgaa agcgagagct ctcatgccaa cctagacggg ctagggccgg
     1621 gccgcgttga cctgaatcaa aggggtggcg tcgatcgatg ccctcaggag cgcgacgacg
     1681 tctttcgtct cctgcggctc gaggatgaag ctcggcatcc tcggatgcgg cgtcgcgagg
     1741 aacgcatgga gagcgctcga cgtcatggct cgcgtattgg caatgtcggc gaagctcggt
     1801 gggatggaga gagatcggcg ggctcagact tccggcgtcg cttccacccg ctcgacgctc
     1861 tcggcggcat cggcaaggtc ggcggccgtg gccgcgagct tgcgcttctc agcgagatcg
     1921 ctcgcggcgc gcgcttcttc ggcgcagagc cgggcctctt cacgcaagtc gtccgcactg
     1981 tagcggccat tgcgacgcat cttcgagaac gccacctctt ggccggcgcg gccgatgcag
     2041 gcatcgatct cggcaaccag cacatgggtc gccgccactt gcgtgtggac tttctccatc
     2101 gttcgctcga tctggtccgc ccactcctcg atctcgcgcg ccagcgccgt caagcggctg
     2161 tccttggggc ggccgcccag cagggacgag acgcggcgat agcgcgcggc gcggtctcgc
     2221 aagccgttga gcgtctcgcg gaggatggct tccctgagct gcatatctgt cgtctcctag
     2281 gaaccctttg gcgcttcgga cggggctttg ccgatcaccg gggccaaccg gcgcgcggca
     2341 tcggtgcgga tggagagtgc aatccggagc aagcgatcgg ccgcatggtg attcaattcc
     2401 ggccgctcgc gcaccatctg cgccacccgt tcgagctcct gcgcgatgtc ttcgtcactg
     2461 ctcagcgacg gtagcgtcgg atcatggctc atgcctcgag cgctccgaaa cggagaagac
     2521 cgatgggtca aactactatt gtttggttaa caagcgctta actcgaaggg atcccgaagt
     2581 tgatccaggt caaacctacc ggggtgtgtt ccgacttagc ctccaggccg agttttcact
     2641 cttcgcgaat ttcgagattt gagcacaggc gcaccgacca cgccgggaac accgaggcgg
     2701 aggtctacgg cgccgtcgat ctgccgttcg ttccgccgga gctgcgcgag aaccgcggcg
     2761 agatcgaggc cgcggcagcg ggccgcctgc cgccgctcgt cgagcttggc gacctgcgcg
     2821 gcgacttgca cacccagagt acggcgacgg acggccacaa tgcggtcgcc gagatggcgg
     2881 aagcggcacg cgctgcgggg ctcgaataca tcgcggtcac cgagcattgg cgccgcatcg
     2941 ccatggcgcc tggcctggac ctggcgcgcc ttcgcgacca gatcgccgag atcgaccgcc
     3001 tcgatgccga aggctcgggt attgctttgc tcaaggcgat cgaggtcgac attctcgagg
     3061 acggcaagct ggacctgccc gactcgatgc tgggcgagtt cgacttggtc gcggcggcga
     3121 tccacagccg gttcaatctg acgcgcgagc ggcagacgga gcgcattctg catgcgatgg
     3181 accggcccta tttcactatc ctcgctcatc cgatcggccg gctgatcggc gagcggccgc
     3241 cttacgacgt cgacatgccg cgcgtcatcg ccgcggcgca tgcccgcggc tgcttcctcg
     3301 agctcaacgc gcatcccgag cggctcgagc tcagcgaggt ccatttgccg catggcgaag
     3361 gaggcgggcg tgctcgtcag catcgccacc gacgcgcatc gcgccgccga gttctcctat
     3421 ctgcgcttcg ggatcggcgc ggcgcggctg gctcgggccg gacgacgtgc tgaacacgcg
     3481 ctcgctcgcc gatctgcgcg tcctgctgaa gaagaccaga gcctagccgt tcgacgacgt
     3541 caggcggctt gcgccagttc ctcgagcgcg tcgatgtcgc tcaccagcac atggctggcg
     3601 ccgcgcagct cgatgacgcg ctgactcctg agctggccga agatgcggct caccgtctcg
     3661 gtggtgaggc cgagatgatc ggcgatgtcg ctgcgtgtca tcggcaggga cacgtgctcg
     3721 gcatcatggc cgcggtcggc catcagcagc agaaacgagg cgatgcgctc gatggcgttc
     3781 ttgcggccga gaagcagcat ctgctgctgt gccgacgaaa gatcccggca caggagctcg
     3841 acctgccatt tgccgagcga cggtcgcagc gagatcatct tttcgatggc gatgcgcggg
     3901 aatcgcaaca gcgtagcgtc ggtcaccgct tccgcggtga aaggataagc cggctcggcg
     3961 gccaggccga tgaaatcgcc gcgcaggaag aactcggcga cgtgtcgccg gccgtcgccg
     4021 aggaggcggc agctccgcac ggcgccggcc accaccttga agcaatactg ggccgcgtcg
     4081 ccttcgagga acaacccttc gtcgcgcccg agcgaaatct ctttgccgat gcggtcgagc
     4141 gccgccagcg cctcgctccg tgccgacccg gcgagggccg acagcggcgg ccgcccgggt
     4201 gaacttggct ctgttgccgc gacggctgct gacatcgatg gtctccggtg cttggacggt
     4261 ggacggccga tcgatactgg cgcatcgtcc gcgcgcggaa gagtcaggag tagtacgtaa
     4321 gggcttctac gtacccgcgg ttgcaggcct gcacccgcat gtggacagca acgcgtgatc
     4381 gacgcgctcg aattcggaat ccgctggttt tcggcgggtt cagcgatatt ccacgagatc
     4441 cgccatgggg aggcggtgct tcgatggttg cggctcgacg cgatggccga gcgccagcag
     4501 caacgcgact tcgactctgg tgcggtcgat gccaagcacc tcggcgacgg cttccggctc
     4561 gaacccgccc atgggacagc tgtcgaggcc gatcacggcc gcggccaaca tcatgtcggc
     4621 ggcggcgatg tggcattggg cgatgctcca cgccgcgacg tcgccgcgcg cgatatcatg
     4681 atagatctgc atcgcctcgt cgagcagctc cggcgaggag acgagccggc ccagcatctt
     4741 ctgcgcatag ccgctgcccg gcgcgagctc ggccttgagg gcgaggatga cgatcatcgc
     4801 gctcgccgtg gtgatctgcg tctgattcca gcaggccggg cgcagccggc ggcgcagcgc
     4861 gcgatcctcg atcacgatga agcgccacgg ctcgaggccg agcgaggacg gcgagaggcg
     4921 ccccgcttcc agaacatagt cgagatcggc gcgcgccagc gccttcgtct cgtcgaacag
     4981 cttgcaggcg tgacgaaact cgagcgcccg gctgaattcc tgtcgcatcg cggcttctcc
     5041 gactgaaagg ctgccgcacc ataaagcagc cggtcgcgtg cggaattgat gtacctcaat
     5101 agcggcggga cgcgcgcgtt acttcaatgt cgcggcgatc tggcggatct cggccggcgt
     5161 gtgctcgccg cccagcggcg tccccggcac cagatatttg cccatggcga gaccgccgac
     5221 gagcacgggc tcgaagccga tttccttgat caggcgcgtc gccaaggcga tcgccttctc
     5281 ctcgtctccg gcgatcggga tgccgagcgg ctcgcccgtg cgatgcgcat tcggcgtgag
     5341 gctccggtag ctgatggcat tgaaggcgcg cacgatatgc gcgcccggca gcagcttcgc
     5401 cgtgacgaca ccggggccgc cttggtccgc tattccctgg acgaaggcgg cgccgtcgcg
     5461 gagcgggatc gggttggaga cgtcgaggac gagttgcttg ctggcgatcg ccttgccgtg
     5521 gtcgtggccg atctcctcga ccgccgggta gggcacggca aggaggatga cgtcgccgaa
     5581 ggcgacggct tgtgcgacgg tgcccgcctg tgcggtcggc ccggcatcgg cgacgaggtc
     5641 tttgaggctc tcggggttgc gcgacgagaa catcacggga tgccccgcct tcgcgaagag
     5701 cgtgccgagc gcgccgcctt cgtggcccgc accgatggtg ccgatcttga gcggcgcgcc
     5761 gccggcggtc tgcgcgaagg cggcaagcgg cgatgcggcg acggcgccga cgacgaggct
     5821 ggtggccgcg agaaaagcgc ggcggctggg tttgcaggac gagtcctcgg tcatgatttc
     5881 tcctcgcggc gacgggtggc gccgactata gcccgctcgc cgcttttgcg ccgctcacga
     5941 tcacgccagc cgatgtgacg gcggcagcgg ttagcgtttg gacgcgccgg gcacgcgctc
     6001 ggcgttttgg cggtcaatca tccagcgggg atattcgggc ttgagggcgc tcgccttgtc
     6061 gagagttgcg agctcatcct cggtgaggcg cagctccgtc gcggcaatgt tgtcctgcag
     6121 ctgctcgatg gtcttcgcgc cgatgatcac gctggtcacg tgtttctggt ggagcagcca
     6181 ggcgagggcc acgcgcgcca ccgaggcgcc gtgccgctcg gcgaccggcc gcatgacgtc
     6241 gacgatggcg aagacacgat cgcggtcgac cggcggaaaa tcgaagctgg tgcggcgcgc
     6301 atcgttggga ccggcgcctt cacgcttgaa cttgccgctg agcagcccgc cggcgagcgg
     6361 gctccacacc atcaggccca gcgcctctgc ggtcagcagc ggcacgatct cgcgctcgat
     6421 gtcgcggctg gcgatggagt agtaagcctg gaccgtctcg aatcgcgcca actcgcgggc
     6481 ggcagagagt cccagcgcct tcgcgacctg ccaggccatg agattggagc aaccgatgta
     6541 gcgcacgaga ccgccgcgga ccaggtcgtc gagcgcgcgg agcgtctcat cgatgggcgt
     6601 cacgatatcg gcgccgtgga tctggtagag gtcgatgtag tccgtgtcga ggcgcttcaa
     6661 gctctcctgg accgccgtca tgatgtgccc gcgcgagaga ccgacggcgt ttgggccagg
     6721 gcccgtgcgt ccccgcgcct tggtggcgat cacgagatcg gcgcggtttg cgccgatgtc
     6781 cttcaaggcc cggccgagaa gacgctcgga ctcgccttcg gaatagacat ccgccgtgtc
     6841 gaggaagttg acgccggcgt cgagggcggc gcggaccagc gccgtggtgg cgtcctggtc
     6901 aagctgcccg atcgcccgcc agaagcctcg tccgccgaag gtcatggtgc cgaggcagac
     6961 ctcggagacg aagaggccgg tgcggcccag aacgcggtat ttcattggcg agtccccggt
     7021 gacgacgttg tgcgaggact gagataaagg cacgccggcg cctcggcaag gggtgcatcc
     7081 gcgatcgccg cgctgccgga acgcgggcta cccgccgcct gctcgttcca gctcggccag
     7141 aatgccgcgg cgcttcgctt gtgcagactc agggaacgtc gcgtctcgct tggccgatcg
     7201 gcggtttttg caggtttgcg cgggcgcgca aggccgcgat gcagaggccg atgggcggcg
     7261 tctcggcctc ggcctcgcag tattcgctcg acagaaccat gccgatggca ttgttgaaga
     7321 cgacggcgcg gatcgttggc ggcgtggcgc ggtcgctgcg gcacagcacg cgaagattcc
     7381 agctccagcc ctccggcacc agctccagcg ccgcgtcgat cgatgtcgtg tagtgatgaa
     7441 cccgactcgg cgcgtcgagc tccatgacgt cgctgccgct ggcagcatga tcgaacgccc
     7501 agaagcagtc gtcgcggcga tctaccgtcg ccacagtctc gcagatcaag cggtccaatc
     7561 ccgccgatcc attcttcgcc gcctccagaa cctggatcag ttcggccaag gtcatggaac
     7621 aatctcgtct gtccaacgcg accagggcgc caacggaagt ggccataatg tcgagctcgg
     7681 atggttgggc accatggtcc ctcctcggcc ggtgaagcga catcaggcct gcccgcacct
     7741 gcaccgggcc tctgctattg gttttataac gattctagct tacgtgaaac tttcgctaac
     7801 gcaacaggcg agtgaacgtt cggtcgccgt aattggggcc gaggaggcca gttggctcgt
     7861 gcgacatgcc ggggatgccg accaacacat tgttaattga aaatactggt ttttgttcga
     7921 tctttcaggg tgcaggcaag gccaggacgt gttagctgac gtcgctggcg aagcgtggtt
     7981 gagcgagatg tcggagtcca cagagaggat agggcggccc gggcacggga cagtccctcg
     8041 catcgtgacg gtggtgcttg gcgtggggac gctgttgtcc ctgtcgtgct ggttggcggc
     8101 cgcgcgctgg gccgacgacc tcgaccgcgc ggaatttgtc cggcgggcga cgaatcacca
     8161 actcatcctg caagccggct tccgcgagta tgaggccgat gagcgctgga acgtggtgac
     8221 gacgccggtc gagggcgatc tccgtggcgc gtggcgcccg agtgcgttga ctgttctggg
     8281 cggcggtctc ctcgccgcgg ccttgggcgc cctactcgcg cgacggcgag cgcggcgcca
     8341 agcggaactc gagcgcctcg cggcaaatct ccgcgtcacc acggcgcgtc tcgtcgagca
     8401 agcggaacag cgcgtcagct atctcgccgg tcatgacgtc gtcaccgggt tgcccaatcg
     8461 caccttcttt gtcgctcgcc ttgccgagca cctcgaggaa tttcggcgtg gcgcctctcc
     8521 tttcgccgtc cactatctcg atctcgatca tttcaaggac gtcaatgaca cgctcggcca
     8581 tgacgccggc gacgagttgc tcaaggagat cggacagcgc ctgctccgcg cggttcgcac
     8641 gaccgacgtc gtcgccagat tgggtggcga cgagtttgcc gtgctgcaag ccgatacgaa
     8701 aatgcccgac gatgcggcgg cgctcgccgt caagctcagg gacctcgtca ccgcgcccta
     8761 tatcctcgcc ggcaaccaca tcgtgacgac ggcgagcatc ggcatcgcgc tcagcaccgg
     8821 cgataccgcg gatgcagcca cgatgatgaa gcaagccgat atcgctctct acaaggcaaa
     8881 gggagaaggc cgcgaccggt tctgcttcca ttccgaagag ctggggaaag cggtccgtgc
     8941 gcgcgttgcg ctggcgtcgg agctgcgtcg ggccgtcgcg gagcacgaat tctttctcga
     9001 gtatcagccg caattcgcgc tcgccggcga cgagctcgtc ggcgtcgagg cgctgctgcg
     9061 ttggcggcat ccggccaaag gggtggtcgc gcccgacctg ttcatgcccg cggccgagag
     9121 ctcgggtctc attctcccga tcggtgattg ggtgttgcgt gaagctttgc ggcagggccg
     9181 cgcgtggatc gacgcgggct gcgcacccac cacgctcgcg gtcaatgttt cggccatcca
     9241 gatcaaggca agcgacttcg aggcgcgctt gctggaggcc ttgcgggata gcggcttccc
     9301 ggcacggtgc ctcgagttgg agctcaccga gacagtccta ttcgagacga cgaaagttca
     9361 tgagcagata ctcgtgcgat tgcacgacgc cggcattcgg gtcgccatcg atgatttcgg
     9421 caccggtttc tcgtctctcg cctacctgat gaggttccat accgaccgcc tcaagatcgc
     9481 gcagacattc gtccgccaca tcgccaccga cgagcatgct gcgtcgatcg tcaaagcggc
     9541 ggtcagcctg gcgcaggccc tcgggctgtc ggtcgttgcc gagggagtgg aatcgccgga
     9601 acagctcgag atgttgcgcc gcttcggctg tgacgcggtg cagggctacc tctacgcgcc
     9661 gccgttgtcg gccgatggcg ttgccgacct ggttcgcgcg cggcgcagcg cgacgctggc
     9721 actggcggca gaatgagcgt catgcgaccg acgcggagaa cctgcgatcg attgcggcga
     9781 ctggtccggg tcttgcgttc atcgccgcgg acatgcgcaa cgattcgctg ctccagctcg
     9841 cggagcgtgc caatcgcctg caggtgctcc tgaccgtgcc gacgatgatc ttcatcatgc
     9901 cgaccatttt cctcgtcgtc ggcgggccga ctgccttgaa attaatcgat ctgctctggt
     9961 gctgatcgca caggcgcgcc gcgcctcgat ccttgtccct tgaggaataa gcgaatccga
    10021 cggcgcgccg gcagtcatgt ctggcgccgt tcttgcatgc aatccgggtc gacacttgaa
    10081 atcgcttgtc gcgcgatcgg cgccggtcgt cgagggagtt gcttatcctc ctgacgggcc
    10141 cgaggcgcgc gggcggagga aggcaggcat gacgcccgtc gacgccgagc cctacagctt
    10201 cgatctcgat ccggcgcacg ccgcgctgct gatcatcgac atgcagcggg atttcctcga
    10261 gcccggcggc ttcggcgaaa tgctcggcaa tgacgtatcg cagctgcgcc gcgcgatcga
    10321 gccgaaccgg cgtctgctcg aggcgtggcg cgcggccgat ctcctcgtca tccacacgcg
    10381 cgagggacat cgtccggatc tcgccgatct ccccttggcc aagaaggagc gcgggcgcgg
    10441 cgacaaaacg atcggcgacc gcggtccgct cggccgcatc ctcgtccgtg gcgagcccgg
    10501 ccatgacatc atctccgagc tctacccgcg cgccggcgag ccggtgatcg acaagccggg
    10561 gaaaggcgct ttcttcgcca ccgatctgca cgccatcctc gacattcgcg cgatccgtca
    10621 gctcgtggtc acgggcgtca cgacggaagt gtgcgtcaac accaccgtgc gcgaggccaa
    10681 tgatcgcggc ttcgattgcc tcgtgatcga ggattgcgtc gcttcttatt ttccggaatt
    10741 ccaggccgcc ggcctcgaca tgatcaaggc gcaaggcggg atcttcggat gggtggctca
    10801 ttcggcagac gtgatacgag cgatcggccg gcgctgaccg cgcctccgga tcgcgcaacc
    10861 cggctttgaa agggggcaac atggcatcga tggcagtcgc acaagcgaag cccaagctct
    10921 gggttcccgg agactggaac gcgttcttcg gtttcggcac caacatcctc gtcaatgtgc
    10981 tgaccttgac ggcgctgttg cgcttcgtcc tgaagatgcc ggattcgctg gtattcggca
    11041 ggatcctgcc ggcgaccggc ctgatgctgt gcctgagcac gctctactat gcgtggctcg
    11101 cctatcgcct cgcgcaaaag acgggccgca gcgatgtctg cgcgctgccc tcgggcacca
    11161 gcgtgccgca catgttcgtg gtcgtgttcg tggtcatgat gccgatcctc ggcaagaccg
    11221 gcgatcccgt gcaggcctgg gaagccggcc tgacttgggt cttcgtccag agcttcgtcc
    11281 tcatggcggg cggcttcatc gcaccggtca tccgcaggat cacgccgcgt gcggcgctgc
    11341 tcggatcctt ggccggcatc tccatcacct tcatctccat gcgcccggcg tcgcagatgt
    11401 tcgtgacccc ggtgatcggc ctcgtctgct tcgccctcat tctcgtcagc tggttcggcg
    11461 gcgtgcgcta tttcaagggc gtgccggccg gtctggtcgc gatcggcgtc ggcaccatca
    11521 tcgcctgggg ctccaacttc tatggccttc actatggcgg gttaagtgtt ggcgcattgg
    11581 cggatgcggt cgccaatttc ggcttctcct atccggttcc cgccctcgcc cacgtccttg
    11641 gtggcttcaa gttcctcggc gtcatcctgg tgacggcgat tccttttggc atctatgacc
    11701 tcgtcgaggc catggacaat gtcgagagcg ccgccgcggc cggcgattcc ttcccgacga
    11761 cgcgcgtgct caccgccgac ggcgtgatca gcctgatcgg ctgcctcatg ggcaatccct
    11821 tcatcaacgc cgtctatatc ggtcatcccg gctggaagtc gatgggcggc agaatcggct
    11881 actcggccgc gaccggcatc ttcgtgatcc tcctctcctg gctcggcgtc gtcgccgtca
    11941 tgatgacgct gatcccggtg gtggcgatcc tgccgatctt gctctatatc ggcatgctca
    12001 tcggctcgca ggcctttcag gagacaccca agagccatgc tccggcaatc atcctcgctc
    12061 tcgtgccgca aatcgctgcc tggggcaagc tccagatcga cggcgcgctg ggcgccgccg
    12121 ggacgaatgc cacggcgctc ggcttcgaca agctcgagca ggtgggcgtg ctttatcgcg
    12181 gcctcgaggt cctgggcggc ggtgccacgc tggccggcat cgtgctgggc gccgtgaccg
    12241 tgttcgtcat cgagagccag ttcctcaagg cttcggcttt cgcgctggcc ggcgccgtgc
    12301 tcaccttctt cggcttgatc catagcgagg ccatcggcat cggcagctcg ccggccgttg
    12361 ccgtgagcta tcttggcatc gccgccttcc tcttcggctg tgcccggttc gccaaggtgg
    12421 taccgcagat ggcggcaatg ccgcatgcgc atcggctgag cacggagccc gccgagtagg
    12481 agaaagcgag gagacatggg gaccacgcct ttcgatgccg gcacttatgt cgatgctgcc
    12541 gcggcggcaa tcgacctgcc gatcgcaccg gaacatcgtc caggcgtgat cctcaatctc
    12601 gagcgcctgg cggcgatggc gcagctcgtc atggcgtttc cgcttgcaaa agagaccgct
    12661 ccggcgcccg tctttcgcgc atgatcggtg ccgggagtga cgcggtcgat attgccgctg
    12721 ccgtaagcgc tggccgcgtg agcgcgcgtg cggtcacgga tgcggcactc gaccgcatcg
    12781 ccgcgggcaa tcagcgcctc aacgccttca cggccgtgac cgcggagcgt gcccgcaaag
    12841 ccgcggcggc catcgacgcc aagcgggcgc gcggcgaagc gctggggttg ctggccggcg
    12901 tgccttacgc cgtgaaaaat ctcttcgaca tcgcgggcgt gacgacgctg gcgggagcga
    12961 agatcgaggc cgggaaagcg ccggccgcgg ccgatgccgc ggcagtgcgc cgtctcgacg
    13021 cggcgggcgc cgtgctggtc ggcgccctca acatggacga gtacgcgtac ggcttcacca
    13081 ccgagaacgc gcattacggg ccgacgcgca acccgcatga cgtcttgcgc agcgccggcg
    13141 gctcctcggg cggctgcggc gccgcggtcg cgggcggcct ggtgccattg tcggttggca
    13201 ccgacacgaa cggctcgatc cgcgtgcccg cctcgctctg cggcattttc gggttgaagc
    13261 cgacctatgg ccgcctcgat cgcgccggcg cgatggcctt tgtcgacagc ctcgaccatg
    13321 tcggtccttt tgcccgctcg gtccgggatc tcgccttgtg ctacgacgcc ttgcagggcg
    13381 ggacgagcgc ccccgtcgag ccgaccgtgc cacggcttgg cgatggcgtc gccgggctca
    13441 gagtcgccgt cgccgacgga tatttcgcgc cggccggcgc cgacgaggcg caggacgccg
    13501 tcgcgcttgt cgctcgggtg ctcggagcga cgcgccgcgt cgagctgccg gaatcggcgc
    13561 gcgcgcgtgc cgccgccttc gtcatcagcg ccgccgaagg cgccaatctc catcttccgg
    13621 acctgcgccg gcgtgcagcg gatttcgatc cggcgacgcg cgaccgcttc ctggccggcg
    13681 ctcttgttcc tgccgcctgg ctgctccagg cgcagcgttt ccgccgctgg taccgcgcgc
    13741 aggtgctcga ggtctttgcc tccactgacg tgatcctggc gcccgcgacg ccctgcgcgg
    13801 cgccgcgcct cggccaggag acgatcatgc tcgagggcaa ggaaatcgcg gtgcggccga
    13861 atctcggcct cttcacgcag ccgctgtcct tcatcggttt gccggtcgtc gtcgtgcccg
    13921 tgcggcggga cggggcgttg cccatcggcg tgcaaatcat cgccgcacca tggtgcgaag
    13981 cgatggccct gcgcgttgcc gcggcgctcg aggatgccgg cgcagtgagc gccaggcttg
    14041 cagggtgacg caggggcacg cggcgttccg gagagccggc atgtacgaga tcaacattcc
    14101 ccagatcgtc gccgaggtga cggccgcctt tgcccgctac gagcaggccc ttgtgaccaa
    14161 tgatattgac gtgctgaacg agctcttctg gaactcgccg cacacgcttc ggctgggggc
    14221 gacggagaac ctctatggct accaggccat caccggcttc cgggcggccc gctcgccgct
    14281 caatctggcg cgccgtttgc gccgcacggt
//
LOCUS       DASYNS010000088        11389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5326219, whole genome shotgun sequence.
ACCESSION   DASYNS010000088 DASYNS010000000
VERSION     DASYNS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11389
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5326219"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..449)
                     /locus_tag="VGP50_02800"
     CDS             complement(9..449)
                     /locus_tag="VGP50_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEV8026329.1"
                     /translation="MERQLLSILASTVLAIHLGVILFNVFGLVAIPLGAWRGWRFVRV
                     FWWRALHLAVLAVVALQALLDRACFLTLWQYALRQGAGEAASPAPLIESWGDRLIFWP
                     LPLWFFAALYVGVSIYTLLLWRLVPPVLPGWTRRITPRRRPPPA"
     gene            497..973
                     /locus_tag="VGP50_02805"
     CDS             497..973
                     /locus_tag="VGP50_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV8026330.1"
                     /translation="MAKQSAGVLLYRVRERRLEVFLVHPGGPFWAGKDVGAWSIPKGE
                     AGPGEDLLTHAKLEFAEETGFAAPNALQALRPIKQAGGKVVHAWAAEGNVDAAALRSN
                     SFTLEWPPRSGRQQIFPEVDRAAWFTLAEAREKINKGQIALLDQLEQLLATRANPD"
     gene            975..1463
                     /locus_tag="VGP50_02810"
     CDS             975..1463
                     /locus_tag="VGP50_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEV8026331.1"
                     /translation="MPTRPRSLDAIDIKILAALQREGRSTIQKLADRVGLSARPCLER
                     VRRLEAAGIIVGYQAVLALEKLAKPITVFAEIALESHGRQDQFERRLAAIEEVVECWE
                     VSGPFDYLARVVCADVARYETLTADLIDDPKLGVARIVSIIALRPVRRFAGYPVSLLT
                     RS"
     gene            1593..2816
                     /gene="rocD"
                     /locus_tag="VGP50_02815"
     CDS             1593..2816
                     /gene="rocD"
                     /locus_tag="VGP50_02815"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEV8026332.1"
                     /translation="MDAVALERQFSAPNYHPLPVVLTRGEGAYLWDVEGRRYIDMMSA
                     YSAVSHGHGHPRILAALEAQARRLAVPSRAYHNDRLGPFLEKLCALTGLDAALPMNSG
                     AEAVETAIKAARRWGYRVKAIARDRAEIIVAESNFHGRTTTIIGFSSEEAYRDGFGPF
                     APGFRAVKFGDLAAMERAITPETAAVLIEPIQGEAGIIVPPKGWLAGVRRLCDKHGVL
                     LIVDEVQSGLGRTGAWFAFQHEQIRPDGLILGKALGGGVLPVSAFVGTRALMDVFTPG
                     SHGSTFGGNPLAAAVGLEALCVLEEEGLVERSRVLGEHMLQRLRSIRSPALKEVRGRG
                     LWVGAEIDPAFGSAREACERLLHKGVLSKETHDTVIRLAPPLVIAREDLDWALDRFEE
                     VVRELEPVRGRSKAA"
     gene            2835..3728
                     /locus_tag="VGP50_02820"
     CDS             2835..3728
                     /locus_tag="VGP50_02820"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEV8026333.1"
                     /translation="MPQPQPRILMCRPDHFAVSYAINPWMDPAGWAAQDQRLAGAARR
                     QWQALQRALSAEGVDIDLVPAVAGVPDLVFTANAAVVMDGKALLARFRHPERQREEPH
                     FARAFHALMASGDIETVTYLPDGLVLEGAGDCVWDGTRGLFWMGYGPRSDRAAQSAVK
                     DAFGLEVVALDLADPRFYHMDTTLCPLTQGDVIYFPGAFTSDGLAAIRARIAPDARIE
                     IDADDATTLAANAICLDDTIILSRCSAGLRRNLETRGYRVVETPLEAFLRSGGSAFCL
                     TLRIDRRSSRTLRQAWQRPAA"
     gene            complement(3769..4869)
                     /locus_tag="VGP50_02825"
     CDS             complement(3769..4869)
                     /locus_tag="VGP50_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV8026334.1"
                     /translation="MQYNYAGTAVDLPPPTKPRARSARRLRGAMRRALRQMAPRRSLS
                     LALQGGGSFGAFTWGVLDRLLEKDRIAFDTISGASAGAINAVLLASGLAEGGPRAARE
                     KLDRFWRRMSQTSMLGPFGSFGASMAETAARAGFALTQFVSPYQFNPLGLNPLRDILA
                     EEVDFARLRAASPAHLLIAATRVRDGSLRLFRTHELTLDVVMASACLPQLHHSVEIEG
                     EWYWDGGFSANPPLRQLALDSTTRDLLLVQIMPEVVDQALPRFSRGIARRVREMSINF
                     PLQKEIEALDELCAFCKQQGGLLRPRFCRKLERLRLHRLVAERELPNLRHASALNVEW
                     SFLNRLKRAGGAAAERWLADLRRGHSQPLRES"
     gene            5082..5264
                     /locus_tag="VGP50_02830"
     CDS             5082..5264
                     /locus_tag="VGP50_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026335.1"
                     /translation="MRLLHERLLYDTSGASFIDFSLIAAFVSLVIVAVVAVLGSDLDK
                     PFNAIVGVLNSSVSHR"
     gene            5359..6291
                     /locus_tag="VGP50_02835"
     CDS             5359..6291
                     /locus_tag="VGP50_02835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEV8026336.1"
                     /translation="MATMKAIRIHRFGGPEVMAVEGVPVPEPAGDEILLRVHAASVNP
                     VDYKIRQGKFVGADKLPIALGRDASGTVEKTGPAQRELRRGDALYAMPGFDRGAFAEY
                     VILKANEAARKPERSSHVEAASVPLAGLTAWQGLFDHGGLKEGQRVLIHGGVGGVGHL
                     AVQFAKAKGARVFTTVSRDDLDFVRELGADEAIDYKAQRFETVAHDVDVVFDLIGGET
                     QERSWTVLKRGGILVSTVGQPPREKAAQHGVRTASFMALPNATQLATIGRLIDDGKVR
                     PHIAATFPFAEAAAAEEQLEHGHVRGKIVLRLVD"
     gene            complement(6295..6588)
                     /locus_tag="VGP50_02840"
     CDS             complement(6295..6588)
                     /locus_tag="VGP50_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026337.1"
                     /translation="MTESPPRSEDPDAIVDAIAYVEELHQELTTDNGAPPLGPQNQVV
                     DAILADPALTAYVRAWADRNRVLEARAEPPQRLPIDAVYRSVSELLLRAEKVR"
     gene            complement(6585..7865)
                     /locus_tag="VGP50_02845"
     CDS             complement(6585..7865)
                     /locus_tag="VGP50_02845"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEV8026338.1"
                     /translation="MALLLPTSTSATFAPAGRWRRDLAALAAFAGPTCIASVAYLDPG
                     NFATNIAAGARYGYRLLWVVLIANLGAMLFQALAAKIGIARRQSLAQLCREELHPLAV
                     GAMWIVSEIGAMATELAEMLGASIGLALLLGMPLLLGMIVTGALSWIILALEPRRFRL
                     IEMIIAALIASIGICYIVETILAQPDWHAVAYHSVVPWLDGHDSVILASGIVGATVMP
                     HAIFLHSSLTRNRLPMADAAAGQRLVRASNIDVGLALTLVGLANMAILFVSAAIFLDA
                     PQGAAGIETAYRTLGPIFGGMSAAIFATALLASGLSSSVVGTLAGQRIMQDFIGWQIP
                     LWLRRLATMAPAVAIVAWGVDPTRTLVMSQVVLSLVLPVPLVALVYFTSRRSVMGLFV
                     NRRWTRIAAIAATLMILALNALLVLDTLDMVTPR"
     gene            8082..8831
                     /locus_tag="VGP50_02850"
     CDS             8082..8831
                     /locus_tag="VGP50_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEV8026339.1"
                     /translation="MFFHRKELMLDVKVEDPNVAFGQLLLEQFGGATGELTAALTYWT
                     QSFHVEERSIRDMLQDIALEEFSHLEMVGRLIEQHTEKGDKDPAYKSTLFAIRGIGPH
                     LVDSHGQAWTAAYVNEGGNVVRDLRADIAAEAGARQTYEALIKLAPDAGSKETLTFLL
                     TREVSHTDMFMKALQSLGKLEDPFFGHVKPDETVDLYFNLSKSGDQKDGRGPWNREPH
                     FKYIADPTPRGDDPMEVVNPDDEHKMPQAAE"
     gene            complement(8863..9615)
                     /locus_tag="VGP50_02855"
     CDS             complement(8863..9615)
                     /locus_tag="VGP50_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8026340.1"
                     /translation="MDLQLENRTALVTGASMGIGRGIALALAAEGARLAVVARRKTLL
                     DELAGDIAAARRPPPALIVQDVMATDAAARIRDAAIAALGTVEILVNCAGGSRPLPID
                     ASDERWEEAMTLNFTRLRQLTQALLPGMIERRWGRIINITGKSEPDRINAAFAAKAAV
                     HSWAKGLSREIGRHGITVNSIAPGRIISEQILRNYPEEERRRFSAEEIPVGEYGQPAD
                     LACLAVFLASPVARYITGTVIPVDGGLRRYQF"
     gene            9765..10193
                     /locus_tag="VGP50_02860"
     CDS             9765..10193
                     /locus_tag="VGP50_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV8026341.1"
                     /translation="MVARTIDAIAVRGEGLFLLLGRLAMGALFVPAGYRHLTNLSGFA
                     QYLGGHGLPGPAMAWAIVGAFVEFFGSLAVLVGFKTRYAALALIAFTIGATLIGHPFW
                     AAPEGQLEAQTINFFKNLAIIGGLLFVFVRGAGPISIDRR"
     gene            complement(10219..10344)
                     /locus_tag="VGP50_02865"
     CDS             complement(10219..10344)
                     /locus_tag="VGP50_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026342.1"
                     /translation="MVADQMGPLDGIAVREPNDAEMVGWLERRTDYAVTEKTAAA"
     gene            complement(10562..>11389)
                     /locus_tag="VGP50_02870"
     CDS             complement(10562..>11389)
                     /locus_tag="VGP50_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8026343.1"
                     /translation="GGSGPPLLLLHGNPFTHLSWHAVAPVLARDFTVVCTDLRGYGDS
                     SKPEGGPDHVNYSFRAMAQDQLEVMAALGFDRFAAAGHDRGARVLHRLCLDHPERVER
                     AAFLDILPQHHLLNNLNLGFAVFSYHWFFMVQPYDFPERLIGADPEYFIRKKLAKTPA
                     GLTFFKPEALAEYIRCIKNPATIHAMCEDYRATIGIDLATDTADVAAGRRVECPVLLL
                     WGATGGVGRHHRPMEVWAPYARDIRRAVALPSGHYLSEECPAGTAAELHAFFAGAAP"
BASE COUNT         1923 a         3698 c         3933 g         1835 t
ORIGIN      
        1 cagcgtcttc atgccggcgg cggccgccgc cgcggagtga tccggcgcgt ccagccgggc
       61 agcaccggcg gcacgaggcg ccacaatagc agcgtataga tagagacgcc gacatagagc
      121 gccgcgaaga accacagcgg cagcggccag aagatcaggc gatcgcccca gctttcgatg
      181 agcggcgccg gcgatgccgc ctctccggca ccctgccgca acgcgtattg ccagagcgtc
      241 agaaagcacg cgcgatcgag cagggcttgc agcgcgacca ccgcgagaac ggcgagatga
      301 agcgcgcgcc accagaagac gcggacgaaa cgccagccgc gccaggcgcc cagcggtatc
      361 gcgacgaggc cgaagacatt gaacaggatc acgccgagat gaatggccag caccgtgctc
      421 gccaatatcg aaagcagctg tcgctccatg gcggctgacc gttgacatcc cgagaggccg
      481 cggctctgtt ggagccatgg cgaagcaaag cgctggtgtg ctgttatatc gcgtccgaga
      541 gcgtcgtctg gaagtgttcc tggttcatcc gggaggaccg ttctgggccg ggaaggacgt
      601 gggtgcctgg tcgattccca agggcgaagc agggccgggg gaggatctgc tgacgcacgc
      661 caagctcgaa tttgccgaag aaacggggtt tgccgcgccc aatgccttgc aggcgctgcg
      721 tccgatcaag caggccggcg gcaaggtcgt gcacgcctgg gcggccgaag gcaatgttga
      781 tgcggcggcg ctccgcagca acagcttcac cctcgaatgg ccgccgcgct cgggccggca
      841 gcagatcttt ccggaggtcg accgcgcggc gtggttcacg ctggccgaag cgcgcgagaa
      901 gattaacaaa ggccagatcg ctctcctcga tcagctcgag cagctcctcg ccacccgcgc
      961 gaacccggac tgacatgccg acgcgtccgc gcagcctcga tgcgatcgac atcaaaatcc
     1021 tggcggcgct gcagcgcgaa gggcgcagca ccatccagaa attggcagac cgcgtcgggc
     1081 tttcggcgcg gccctgtctc gaacgggttc gccggctcga ggcggccggc atcatcgtcg
     1141 gctatcaggc ggtgctggcg ctcgagaagc tggcgaagcc gatcaccgtg tttgccgaga
     1201 ttgcgctcga gagccatggc cggcaggatc agttcgagcg ccgtctcgcg gccatcgagg
     1261 aggtcgtcga gtgctgggag gtgagtggcc ccttcgacta tctggcgcgg gtcgtctgtg
     1321 cagacgtcgc ccgctacgag acgctcaccg ccgacctcat cgacgacccg aagctcggcg
     1381 tggcgcggat cgtcagcatc attgcgctgc gcccggtgcg ccgcttcgcc ggctaccccg
     1441 tctcgctttt gacgcgctcc tagacccggc gcagccgttt ctgtcgcaaa agcgcgtcaa
     1501 aaacgccgga tctgtcgcgg ggggttagga cttcggccac aactgtggcg gcagactcgg
     1561 catgatcaat cgactccgag gaggcccgcg ccatggacgc cgtcgcgctc gagcgccagt
     1621 tcagcgctcc caactaccac cccctgccgg tcgtgctgac gcgcggcgag ggcgcttatc
     1681 tttgggacgt cgagggccgc cgctacatcg acatgatgag cgcctattcg gcggtcagcc
     1741 atggccacgg ccatccgcgc attcttgcgg cgctggaggc gcaggcgcgc cgtctcgccg
     1801 ttccctcgcg cgcctatcac aacgatcgcc tggggccctt tctggagaag ctctgcgcgc
     1861 tgaccgggct cgatgccgcg cttcccatga acagcggcgc cgaggcggtc gagacggcga
     1921 tcaaggcggc gcgccgctgg ggctatcgcg tcaaggccat cgcccgcgac cgcgccgaga
     1981 tcatcgtcgc cgaaagcaat ttccacgggc gcaccacgac catcatcggc ttctcttcgg
     2041 aggaggccta tcgcgacggc ttcggacctt tcgcgcccgg cttccgcgcc gtgaaattcg
     2101 gcgatctggc ggcgatggag cgcgcgatca cgccggagac ggcggcggtt ctcatcgagc
     2161 cgatccaagg cgaagccggc atcatcgtgc cgcccaaggg ttggctcgcc ggcgtgcgcc
     2221 ggctctgcga caagcatggc gtgctgctga tcgtcgatga agtccagtcg ggcctcggcc
     2281 gcaccggcgc gtggtttgcc ttccagcatg agcagatccg ccccgacggc ctcattctcg
     2341 gcaaggcgct gggcggcggc gtgctgccgg tgtcggcgtt cgtcggcacg cgcgcgctca
     2401 tggatgtctt cacgccgggc tcgcatggct cgaccttcgg cggcaatccg ctggcggccg
     2461 ccgtcggcct cgaagcgctc tgcgttctcg aggaggaagg gctggtcgaa cgcagccgtg
     2521 tgctcggcga gcatatgctg cagcgtctgc gatcgatccg cagcccggct ctcaaggagg
     2581 tgcgcggccg cggcctgtgg gtgggcgccg agatcgatcc cgccttcggc agcgcgcgcg
     2641 aggcctgcga gcgcctgctg cacaagggcg tgttatcgaa ggagacccac gacacggtga
     2701 tccgcctggc gccgccgctg gtcattgccc gcgaggatct cgattgggcg ctcgatcgct
     2761 tcgaagaggt cgtgcgcgag ctcgagcccg tgcgcggccg ctcgaaggcc gcctgaggga
     2821 cgaggacgcg agcgatgcca cagccacagc cgcgcatcct catgtgtcgg ccggaccact
     2881 ttgccgtcag ctacgccatc aatccctgga tggatccggc cggctgggcg gcgcaggatc
     2941 agcggctcgc cggagccgcg cggcggcaat ggcaagccct gcagcgcgcg ctttccgctg
     3001 aaggtgtcga tatcgatctc gttccggccg tggccggcgt gcccgacctc gtctttaccg
     3061 ccaatgccgc ggtcgtcatg gacggcaagg cgctgctcgc gcgcttccgc catcccgagc
     3121 ggcagcgcga ggagccacat ttcgcgcgcg cctttcacgc gctgatggca agcggcgaca
     3181 tcgagaccgt cacgtacttg cccgatggcc tggtgctcga aggggccggc gattgcgtct
     3241 gggacggcac gcgcggtctt ttctggatgg gttacggccc gcgctcggat cgtgccgcgc
     3301 agagcgccgt caaggatgcg ttcggcctcg aggtcgtggc gctcgatctc gccgatccgc
     3361 gcttctatca catggacacg acactttgcc cgttgacgca gggcgacgtc atctactttc
     3421 ctggtgcgtt cacgagcgat ggcctcgccg cgatccgcgc gcggatcgcg ccggacgcgc
     3481 ggatcgagat cgacgcggat gacgcgacca ctctggcggc gaacgccatc tgcctcgacg
     3541 acacgatcat cctgtcgcga tgcagcgcgg gcctgcgccg caatctcgaa accaggggat
     3601 accgcgtcgt cgagacgccg ctcgaggcct tcttgcgcag cggcggctcg gccttctgct
     3661 taaccctgcg tatcgaccgc cgctcgagtc ggacgctgcg ccaggcgtgg cagcggccgg
     3721 cggcgtagcc ggcgccgcgc gcgtattcgg gcggtatctc ctcgacgtct aagactcgcg
     3781 caaaggctga gagtggccgc ggcgaagatc ggcgagccag cgctcggcgg cggcgccacc
     3841 ggcccttttg agccggttca ggaaggacca ttcgacattg agcgcgctgg catggcgcag
     3901 gtttggcagc tcgcgctcgg cgacgaggcg atgaaggcgc agccgctcga gtttccggca
     3961 gaagcgcggg cgcagaagtc cgccctgctg cttgcagaag gcgcacagct cgtcgagcgc
     4021 ctcgatctcc ttctgcagcg ggaaattgat gctcatctcc ctcacccggc gcgcgatgcc
     4081 gcgcgagaat cgcggcagcg cttggtcgac gacttccggc atgatctgca cgagcagcag
     4141 gtcgcgcgtc gtcgaatcga gcgcgagctg ccgcaatggc ggattggcgc tgaagccgcc
     4201 gtcccagtac cactcgccct cgatctcgac gctgtgatgg agctgcggca ggcaagccga
     4261 cgccatgacc acatcgagcg tgagctcatg ggtacggaag agccgcaggc tgccgtcgcg
     4321 cacccgggtc gcggcgatca gcagatgcgc tggcgaggcc gcgcgcaacc gggcgaagtc
     4381 cacctcctcg gccaggatgt cgcgcagcgg gttgaggccc agcgggttga attggtaggg
     4441 cgacacgaac tgcgtcagcg cgaagccggc gcgcgccgcc gtttccgcca tgctggcgcc
     4501 gaaggaaccg aacggcccga gcatgctcgt ctggctcatg cggcgccaga agcgatcgag
     4561 cttctcgcgc gccgcgcgcg ggccgccttc ggcgagcccg ctggcgagta ggacggcatt
     4621 gatagcgccg gcgctcgcac cgctgatggt gtcgaaggcg atccgatcct tctcgaggag
     4681 acggtcgagc acgccccagg tgaaagcgcc gaaggagccg ccgccttgca gcgccagcga
     4741 cagcgagcgc cgcggcgcca tttgtctgag cgcacgccgc atcgcgccgc gcaggcggcg
     4801 ggcggagcgg gcgcgcggct tggtgggcgg gggcagatca acggcggttc cggcataatt
     4861 atattgcatc gcaaacacct ctcggccggg atcctagagc cgggagtatg gataaagtgt
     4921 gactaggatg aatcatattg ctgcaatgca gcgtagtaca agtggaatgt agttctcgcc
     4981 cgaacattcg gtattttagg aaaattttaa tagattgttg tggcttcaac gttaagcccg
     5041 cgttaagaat tgccgaaagg gcgggggctc ggaggatgac tatgcggtta cttcatgagc
     5101 gcctgcttta cgataccagc ggcgccagtt tcatcgactt tagtctgatc gcggccttcg
     5161 tttcgctcgt catcgtcgcg gtcgttgccg tgctcggcag cgacctggac aagccgttca
     5221 acgccatcgt cggcgtgctc aacagcagcg tcagtcaccg ctgaccgtcc gttgccgcgc
     5281 cccggaatcg acggccgcgg cggatttgtg tcgcgccgct gacgttcgct gactgcaatc
     5341 cgtgatcggg aggcagtcat ggcgacgatg aaggcgatac gcatccaccg cttcggcgga
     5401 cccgaggtaa tggcggtcga aggagttccg gtcccagagc ccgccggcga cgagatcctg
     5461 ctccgcgtcc atgccgccag tgtaaatccc gtcgactaca agatacgcca gggcaagttc
     5521 gtcggcgccg acaagctgcc gatcgcgctc ggtcgcgacg cctccggcac cgtcgagaaa
     5581 acgggtcccg cccagcgcga gctgcgccgc ggcgacgcgc tctatgcgat gccgggtttc
     5641 gatcgcggcg cttttgccga gtatgtaatt ctcaaggcga acgaggccgc gcgcaagccc
     5701 gagcggagca gccatgtaga ggcggcgagc gtgccgctgg cggggctcac ggcctggcaa
     5761 ggtctcttcg accatggcgg ccttaaggaa ggtcagcgcg tgctgatcca tggcggcgtg
     5821 ggcggcgtcg gccatcttgc ggttcaattc gccaaggcga aaggggcgcg cgtgttcacg
     5881 accgtgtcgc gggacgacct cgatttcgta cgcgagctcg gcgccgacga ggccatcgac
     5941 tacaaggcgc agcgcttcga gacggtcgcg cacgacgtcg acgtggtttt cgacctcatc
     6001 ggtggcgaga cgcaggagcg ttcctggacg gtgctgaagc ggggcggcat cctcgtgtcg
     6061 accgtgggtc agccgccgcg cgagaaggcg gcgcagcatg gcgtccgcac cgcgagtttc
     6121 atggccctac ccaatgcaac gcagctcgcg acgatcggcc gactgatcga cgacggcaag
     6181 gtgcgcccgc acatcgcggc gaccttcccc tttgccgaag cggccgcggc ggaggagcag
     6241 ctcgagcacg gccatgtgcg cggcaagatc gtgctgcgtc tcgtcgactg atcgctaccg
     6301 caccttctcg gcgcgcagca gcagctcgct gacggagcgg tagacggcat cgatcggcag
     6361 ccgctgcggc ggctcggcgc gcgcctcgag cacgcggttg cggtccgccc aagcgcgcac
     6421 ataagcggtg agcgccgggt cggcgaggat ggcgtcgaca acctggttct gcggcccgag
     6481 cggcggcgcg ccattgtcag tcgtgagctc ctgatgaagc tcctcgacat aggcgatcgc
     6541 atcgacgatg gcgtcgggat cttcggagcg cggcgggctc tcggtcatcg cggtgtcacc
     6601 atgtccaggg tgtcgagcac gagcaaggcg ttgagggcaa ggatcatcag cgtcgccgca
     6661 attgccgcga tccgcgtcca ccgacggttg acgaagaggc ccatgacgct gcgccggctg
     6721 gtgaagtaga cgagcgccac cagcggcacc ggcaaaacga ggctcaatac gacttgactc
     6781 atgaccaggg ttcgagtcgg atcgacgccc caggcgacga tggcgaccgc cggggccatg
     6841 gtcgcgaggc gacgcagcca gagcgggatc tgccatccga tgaaatcctg catgatccgc
     6901 tgtccggcga gcgtgcccac caccgagctc gacaagcccg aggcaagcag cgccgtcgcg
     6961 aagatcgccg ccgacatccc gccgaagatc ggaccgagcg tgcgataagc ggtctcgatg
     7021 cctgccgccc cttgcggcgc gtccaagaag atggcggccg atacgaagag gatcgccatg
     7081 ttggcgaggc cgaccagggt cagtgccagc ccgacgtcga tattggaagc ccggaccagt
     7141 ctctgtcccg cagccgcatc ggccatcggc aaacggttcc gcgtcaggct cgaatggaga
     7201 aagatggcgt gcggcatgac ggtggcgccg acgatgcccg aggcgaggat cacgctgtca
     7261 tggccgtcga gccagggcac gaccgagtga taggcgacgg cgtgccagtc cggttgcgcc
     7321 aggatggtct cgacgatgta gcagatgccg atcgacgcga tcagcgcggc gatgatcatt
     7381 tcgatcaggc gaaaacggcg cggctcgagc gcaaggatga tccagctcaa cgcgcccgtg
     7441 acgatcatgc cgagaagcaa cggcataccc aggagcaacg ccaggccgat gctggcgccg
     7501 agcatctccg cgagttccgt cgccatcgcg ccgatctcgc tcacgatcca catcgcgccc
     7561 acggcaagag ggtgcagctc ctcgcggcag agctgcgcga ggctttgccg acgcgcgatg
     7621 ccgatcttgg cggcaagcgc ctggaacagc atcgcgccga gattggcgat cagcacgacc
     7681 cagagcaggc ggtagccgta gcgagcgccc gccgcgatgt tggtggcgaa gttgccgggg
     7741 tccagatagg cgacggaggc gatgcaggtc gggccggcga aggcggcaag agccgcaaga
     7801 tcacgccgcc atcgcccggc cggcgcaaac gtcgcgctcg tgctggtcgg gagaagcagg
     7861 gccatggcag atcctgtcgg gtttctcatg acgaaccacc gccgcgagat ttcggctcat
     7921 tcccagcacg gcaggcgaaa tgggcggcgc gcgccatcgc gttgcgccat cgctggtcgg
     7981 agatgccgcc ggcggcgcgc caatctcgcg agaagggaac actgctcgcc gcgatgtcgt
     8041 tgggagagcg agatccttcc ctccagtcac ggacaggtgc catgttcttc catcgcaaag
     8101 agctcatgct cgacgtcaag gtcgaggatc ccaacgtcgc cttcggccaa ttgctgctgg
     8161 agcagttcgg tggcgccacc ggcgagctga cggctgcgct cacctattgg acgcagtcct
     8221 tccatgtcga ggagcgctcg atccgcgaca tgctgcagga catcgccctc gaggagttca
     8281 gccatctcga gatggtcggc cggctgatcg agcagcatac ggagaaaggc gacaaggatc
     8341 ccgcctacaa gagcacgctc tttgctatcc gcggcatcgg ccctcatctc gtcgacagcc
     8401 atggccaggc ctggaccgcc gcctatgtca atgaaggcgg caatgtcgtt cgcgacctgc
     8461 gcgccgacat cgccgccgaa gccggggcgc gacaaaccta cgaggcattg atcaagctcg
     8521 ctccggacgc cggatcgaag gagacgctga ccttcctgct gacgcgcgag gtctcgcaca
     8581 ccgacatgtt catgaaggcg ctgcagtcgc tcggcaagct cgaggatccg ttcttcggcc
     8641 atgtcaagcc cgacgagacg gtggacctct atttcaacct ctcgaagtcc ggcgatcaga
     8701 aggatgggcg cggcccctgg aaccgcgagc cgcatttcaa atacatcgcc gacccgaccc
     8761 cgcgcggcga cgacccgatg gaggtcgtca atcccgacga cgagcacaag atgccacaag
     8821 cggcggagtg acttggacga gcggctgaga cgggagggct ggtcagaatt gataccgccg
     8881 cagcccgcca tcgacgggaa tgaccgtgcc ggtgatgtag cgggcgaccg gtgaggcgag
     8941 gaagacggcg agacaggcga ggtctgccgg ttggccgtac tcgcccaccg ggatctcctc
     9001 ggcggagaag cggcggcgct cctcttcggg atagttgcgc agaatctgct cgctgatgat
     9061 gcggccgggc gcgatcgagt tcacggtgat gccgtggcgc ccgatttcgc gcgacaggcc
     9121 cttcgcccag gaatggacgg cggccttggc ggcaaaggcg gcgttgatcc ggtcgggctc
     9181 ggactttccg gtgatgttga tgatgcggcc ccatcgccgc tcgatcatgc ccggcaacag
     9241 cgcctgagtg agctggcgca gccgcgtgaa gttgagggtc atggcctcct cccagcgctc
     9301 gtcgctggcg tcgatgggga gtggccggct gccgcccgcg caattgacga ggatctcgac
     9361 cgtgccgagc gcggcaatgg cggcatcgcg gatacgcgct gccgcgtcgg tggccatgac
     9421 gtcctggacg atgagggcgg gcggggggcg gcgcgccgcc gcgatgtcgc ccgcgagctc
     9481 gtcgagcagc gtcttgcgcc gcgccacgac ggcaagccgc gcgccttcgg ccgccagcgc
     9541 cagggcgatg ccgcggccga tgcccatgct ggcgccggtg acgagcgcgg tccgattctc
     9601 gagttgcagg tccaaggcat ttctcctgcc ggtgagggag tccggtcatt ctaagcccgg
     9661 ctgcctgccg ggatgccgcc cttttaaccg cgcggcgaat gctgtacgaa gtcgccggcg
     9721 cgcagcgttg ctggcacggg gcgcgcgcct actgggggag gggaatggtg gcacgcacta
     9781 tcgatgcgat cgccgttcgc ggcgaaggct tgtttctgct cttgggccgg cttgccatgg
     9841 gtgcactctt cgtgcccgcg gggtaccggc atctgaccaa cctcagcggt ttcgcccagt
     9901 atctgggcgg gcatgggctt cccggcccgg ccatggcctg ggcgatcgtc ggcgccttcg
     9961 tggagttttt cggcagcctc gcggtgctgg tcggcttcaa gacgcgctat gcggcgctgg
    10021 cgctcatcgc ctttaccatc ggggcgacgc tgatcggtca tcccttctgg gccgcgcccg
    10081 aaggccagct cgaagcgcaa accatcaatt tcttcaagaa cttggcaatt atcggcgggc
    10141 tgctgttcgt gttcgtgcgt ggtgccggcc cgatcagcat cgaccggcgc tagcctgtct
    10201 tcgttggcgt ccgtccgctc aggcggccgc ggtcttttcc gtcacggcat agtcggtccg
    10261 gcgctcgagc caaccgacca tttccgcgtc gttgggctcg cgcaccgcga tcccgtccag
    10321 cggacccatc tggtccgcga ccatcagagc ggtcgctatg gcatccgtgg ggttcgcagc
    10381 gcgtaccacg gccagcggtc gctcccctcg ggttaccgta tagatccgca ttgcctcctc
    10441 cgggtcactt ttccggcatt ctactgcaaa cgcgagcggc gggcatcagt gccataaggc
    10501 gagtcgcgct ctccgaatcg ccgcggcacc tcctcccgag ggaggtacgg caaaccgccc
    10561 atcaaggggc ggcccccgcg aagaaggcgt gaagctcggc ggccgtcccg gccgggcatt
    10621 cctcgctcag gtaatgcccg gaggggagcg ccacggcgcg gcggatgtcg cgggcatagg
    10681 gtgcccagac ctccatcggc cgatggtggc gcccgacgcc gcccgtcgcg ccccagagca
    10741 gcagcaccgg gcattcgacg cggcgcccgg ccgcgacatc ggcggtgtcc gttgcgaggt
    10801 cgatgccgat ggtggcgcgg taatcctcgc acatggcgtg gatggtcgcc gggttcttga
    10861 tgcagcggat gtactcggcg agcgcctcgg gcttgaagaa ggtcaggccg gcgggtgtct
    10921 tggccagctt cttgcgaatg aaatactcgg gatcggcacc gatcagccgc tcggggaagt
    10981 cgtagggctg gaccatgaag aaccagtgat aggagaagac ggcgaagccg aggttgaggt
    11041 tattgaggag gtgatgctgc ggcaggatgt cgagaaaagc cgcgcgctcg acacgctcgg
    11101 gatgatcgag gcagaggcgg tggagcacgc gggcgccgcg atcgtgcccg gcggcggcaa
    11161 agcggtcgaa gcccagcgcg gccatcacct cgagttgatc ctgagccatg gcgcggaagg
    11221 aatagttgac gtgatccggg ccgccctccg gcttcgagct gtcgccgtag ccgcggaggt
    11281 cggtgcaaac cacggtgaag tcgcgcgcca gaacgggggc gacggcatgc caggacaaat
    11341 gcgtgaaggg attgccgtgg agcagcagca gcggcggtcc gctgccgcc
//
LOCUS       DASYNS010000089         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1583265, whole genome shotgun sequence.
ACCESSION   DASYNS010000089 DASYNS010000000
VERSION     DASYNS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583265"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1702)
                     /locus_tag="VGP50_02875"
     CDS             complement(<1..>1702)
                     /locus_tag="VGP50_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026344.1"
                     /translation="MYAVAFQHMIASPTAALMLAGVFVVLSQLKINVTNSYAGSIAWS
                     NFFSRLTHRHPGRVVWLVFNVTIALLLIESGVYSALEQILGLYSAVAAAWVGALVSDL
                     VVNKPLKLSPPFIEFKRAHLFDINPVGVGAMLGAVLAATLAYAGLFGPTARAFTPFIA
                     LATAFALAPLIAWATGGRYYLARKPRRNWASAGPIRCTVCENVFEAEDTAYCPAYAGA
                     ICSLCCSLDARCHDGCKPAARLSRQVSGLLAALLPARLVAHVDSRLSQYLGVLALLAS
                     MIGVTLTVVYLQTVPGAEVSAAMIRGAFWKLFFVLFIIAGIGAWLFVLARESRRTAQD
                     ESRRQTTLLLREIRAHQRTDAQLQRAKEAAEAANLAKSRYVIGISHELRTPLNAILGY
                     AQLLERDESIPRHRRDSIRIIRRSGDHLSGLIEGLLDISKIEAGRLRLNLDEVRLADF
                     LEQIVDMFRLQASAKGIGFTFEPPEHLPEVVHTDQKRLRQILINLLSNAIKFTDRGRV
                     VLRLRFRSEVAEIEVEDTGIGIAEADLKRVFEPFERIDPADGMAKPGVGLGLTITKLL
                     V"
BASE COUNT          312 a          512 c          638 g          240 t
ORIGIN      
        1 accagcagct tggtgatggt gaggccgagg ccgacgccgg gcttggccat gccgtcggcg
       61 ggatcgatgc gctcgaaggg ctcgaagacg cgcttcagat cggcctcggc gatgccgatg
      121 ccggtgtcct cgacctcgat ctccgcgacc tcgctgcgga agcgcaggcg cagcacgacg
      181 cggccccgat cggtgaattt gatggcgttc gacaacaggt tgatgaggat ctggcgcagc
      241 cgcttctggt cggtatggac gacctcgggc agatgctcgg gcggctcgaa ggtgaagccg
      301 atgcccttgg cgcttgcctg gaggcggaac atgtcgacga tctgctcgag gaaatcggcg
      361 aggcgaacct cgtcgagatt gaggcggagc cgccccgcct cgatcttgga gatgtcgagc
      421 aggccctcga tcaagcccga gagatggtcg ccgctccggc ggatgatgcg gatggaatcg
      481 cggcggtggc gcgggatcga ttcgtcgcgc tcgaggagct gcgcgtagcc gaggatggcg
      541 ttgagcggcg tgcgcagctc gtggctgatg ccgatgacat agcggctctt ggcgagattg
      601 gcggcttcgg cggcctcctt ggcgcgctgg agctgcgcgt cggtgcgctg atgcgcgcgg
      661 atctcgcgca ggaggagcgt cgtctggcgc cgcgattcgt cctgcgcggt gcggcggctc
      721 tcgcgcgcga gcacgaacag ccaggcgccg atgccggcga tgatgaagag gacgaagaac
      781 agcttccaga aggcgccgcg gatcatcgcc gccgagacct ccgcgccggg gaccgtctgg
      841 agatagacga ccgtcagcgt gacgccgatc atcgaggcga gaagcgccag aacgccgaga
      901 tactgcgaga ggcgcgaatc gacatgagcg acgaggcgtg ccggcagaag cgctgccagg
      961 agaccgctca cctggcgtga gagccgcgcc gccggcttgc agccgtcgtg gcaacgcgcg
     1021 tcgagcgagc agcagagcga gcagatcgcg ccggcataag cgggacaata ggccgtgtcc
     1081 tcggcctcga agacattctc gcagacggtg cagcggatgg ggccggcgct cgcccagttg
     1141 cgccggggtt tgcgcgcgag gtagtagcgg ccgccggtcg cccaggcgat gagcggcgcc
     1201 agcgcgaagg ctgtcgcgag cgcaatgaag ggcgtgaagg cccgcgcggt cgggccgaaa
     1261 agtccggcat aggcgagcgt cgccgccagc accgcgccca gcatcgcgcc gacgccgacc
     1321 gggttgatgt cgaaaagatg cgcgcgcttg aactcgatga agggcgggct cagcttcaac
     1381 ggcttgttga cgacgaggtc ggacacgagc gcgccgaccc aggccgcggc gaccgcggaa
     1441 tagaggccga ggatctgctc gagcgccgaa tagacgccgg actcgatgag cagcagcgcg
     1501 atggtcacgt tgaacaccag ccagacgacg cggccgggat ggcgatgcgt cagccgcgag
     1561 aagaaattcg accaggcgat cgagccggca taggaattcg tcacattgat cttgagctgc
     1621 gacaggacga cgaagacgcc cgcgagcatc agcgccgccg tcggcgacgc gatcatgtgc
     1681 tggaaggcga cggcgtacat gg
//
LOCUS       DASYNS010000090         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4546808, whole genome shotgun sequence.
ACCESSION   DASYNS010000090 DASYNS010000000
VERSION     DASYNS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4546808"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1730
                     /locus_tag="VGP50_02880"
     CDS             <1..>1730
                     /locus_tag="VGP50_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEV8026345.1"
                     /translation="ATTGADEDHSASAAAEAAAEAGDDNNGARAAATARDGAENGDDD
                     EAAEEVVESVGGSDALEEVPDRAPRYRRQYKIQEVIKRRQVMLVQVVKEERGTKGAAL
                     TTYLSLAGRYSVLMPNTARGGGISRKITSPEDRSRLKEIAQELEVPEGMGVILRTAGA
                     SRTKTEIKRDFEYLLRLWETVRDLTLKSTAPKLVYEEGSLVKRSIRDLYSKDIDEVTV
                     AGPEGYDEAKEFMRMLMPSHARNVKLYTDTQPLLSRYGVENQLDAMFSPVVQLRSGGY
                     IVLNQAEALVAIDVNSGRATREHHIEDTALKTNLEAADEIARQLRLRDLAGLIVIDFI
                     DMDEKRNNRAVERRLKECLKQDRARIQVGRISHFGLLEMSRQRIRTSVLESSTDKCPY
                     CGGSGHVRSVSSLALQVLRALEDQLMKSGTHNLIARTRPDVALYVLNQKRAHLRALEE
                     RFAIAITVSADETVTAPQAFVIDRGEQVHTAEAAKALAEQSAAAAPAAPLEEEDELEP
                     AGIADDEEAEADAAEAAEGAPESGEGGPRRRRRRRRRGRNGGEAATHGPSRHEPVVAG
                     AAQPFAEGDE"
BASE COUNT          324 a          574 c          595 g          237 t
ORIGIN      
        1 agcgaccacc ggagcggacg aggatcattc cgcttcggcg gcggccgagg cggccgctga
       61 agctggcgat gataacaacg gcgctcgcgc cgccgcgacg gcgcgggacg gcgccgagaa
      121 cggcgatgat gacgaagccg ccgaagaggt cgtcgaatcg gtcggcggct ccgacgcgct
      181 cgaggaagtc cccgatcgcg cgccgcgcta tcgccgccag tacaagatcc aggaagtgat
      241 caagcgccgg caggtgatgc tggtgcaggt ggtcaaggag gagcgcggca ccaagggcgc
      301 ggcgctcacg acctatctgt cgcttgccgg ccgctattcg gtgttgatgc ccaacacggc
      361 gcgcggcggc ggcatcagcc gcaagatcac cagcccggag gaccgttcgc ggctcaagga
      421 gattgcgcag gagctggaag tccccgaggg catgggcgtg atcctgcgca ccgccggcgc
      481 cagccgcacc aaaaccgaga tcaagcgcga tttcgagtat ctgttgcggc tttgggaaac
      541 ggtgcgcgac ctgacgctca aatcgaccgc gcccaagctc gtctacgagg aaggctcgct
      601 ggtcaagcgc tcgatccgcg atctctacag caaggacatc gacgaggtca cggtcgccgg
      661 ccccgagggc tacgacgaag ccaaggaatt catgcgcatg ctgatgccga gccatgcgcg
      721 caacgtcaaa ctgtacaccg acacgcagcc gctcctctcg cgctacggcg tcgagaacca
      781 gctcgacgcc atgttctcgc cggtcgtgca actgcgctcc ggcggataca tcgtgctcaa
      841 tcaggccgag gcgctggtcg ccatcgacgt caattccggc cgcgccacgc gcgagcatca
      901 catcgaagac accgcgctca agaccaatct ggaggcggcg gacgagatcg cgcgccagct
      961 gcggctgcgc gatctcgccg ggctgatcgt catcgatttc atcgacatgg acgagaagcg
     1021 caacaaccgc gctgtcgaac ggcgcttgaa ggaatgcctc aagcaggacc gcgcccgcat
     1081 ccaggtcggg cgcatctcgc atttcggctt gctggaaatg tcgcggcagc ggatccgcac
     1141 cagcgtgctg gagagttcga cggacaagtg cccctattgc ggcggcagcg gccatgtgcg
     1201 ctcggtgtcg tcgctcgcac tgcaggtgct gcgcgcgctc gaagatcaat tgatgaaatc
     1261 cggcacccac aatctcattg cccgcacccg gccggacgtg gcgctctatg tgctcaatca
     1321 gaagcgtgcg catctgcgcg cgctcgagga gcgcttcgct atcgccatca cggtgagcgc
     1381 cgacgagacg gtgaccgcgc cgcaggcttt tgtcatcgat cgcggcgagc aggtgcatac
     1441 cgccgaggcc gccaaggcgc tggccgagca atcggccgcg gcggcacccg ccgcgccgct
     1501 cgaggaggag gacgagctcg agccggctgg aattgccgac gacgaggaag ccgaggccga
     1561 cgcggcggag gccgcagagg gcgcacctga atcgggagag ggcggcccgc ggcgtcgccg
     1621 ccgccggcgg cggcgtggac gcaacggcgg cgaagctgcc acccacgggc cgtcccgcca
     1681 cgagccggtc gtcgcgggag cggcccagcc ctttgccgaa ggcgatgagg
//
LOCUS       DASYNS010000091         2847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3610701, whole genome shotgun sequence.
ACCESSION   DASYNS010000091 DASYNS010000000
VERSION     DASYNS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3610701"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VGP50_02885"
     CDS             <1..655
                     /locus_tag="VGP50_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEV8026346.1"
                     /translation="LDGAESLSQFVQRLITGRAALAPEYTEADISRDFKSNGTQEPDD
                     EDYQALAKNGFADWRLEVGGLVDHPLRLSLEELRRLPARTQITRHDCVEGWSCIGKWT
                     GAKLGPLLDRAGVKPEARYVVFYCADAMDATGTKYYESIDLESAYHPQTILAYEMNDQ
                     VLPVPHGAPLRLRVERQLGYKMAKYIMRIDLTRGFADIADGGGGYWEDQGYEWYAGI"
     gene            complement(652..1656)
                     /locus_tag="VGP50_02890"
     CDS             complement(652..1656)
                     /locus_tag="VGP50_02890"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEV8026347.1"
                     /translation="MAHGVLVDGVWQGEWYDTSASGGRFVPAAPLYRNWVTPDGGVGP
                     SGGDGFAAERGRYHLYVSLACPFAHRTIIFRKLKRLEDVVSLSLVEPVMGEQGWEFGA
                     GTTADALNGKHRLYEIYLLANPRYTGRVSVPVLWDKKRQTIVNNESPEIIRMLNSAFD
                     ALTADRSDYYPPALRAAIDRANERIFKDVNYGVYRAGFATWQEVYEQAVDDVFAALDW
                     LEERLAHQRYVVGEHMTEADWRLFTTLIRFDAVYYGHFKCNLRRITDYPNLANYLREL
                     YQVPGVADTVSLDHIKRHYYMSMTAINPTRIVPKGPVLDFAAPHDRGRRPSAPQDNRT
                     "
     gene            1804..2274
                     /locus_tag="VGP50_02895"
     CDS             1804..2274
                     /locus_tag="VGP50_02895"
                     /inference="COORDINATES: protein motif:HMM:NF021495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEV8026348.1"
                     /translation="MGDATTDLVLAIAHHLLVFMLAGVLAFEIGTVTPTMKRDEILGV
                     GRVDNGYGILAAGIIVVGFIRATVAAKGWAYYAVNLFFWAKIATFAVVGLLSIVPTVA
                     IIRWRRALKSDAAFAPPADAIRRVRVYLWAEAALFLLLPVFAAAMARGYGELVP"
     gene            2403..2537
                     /locus_tag="VGP50_02900"
     CDS             2403..2537
                     /locus_tag="VGP50_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026349.1"
                     /translation="MKGDAEILRSAPRRRAAARSGAAPERIETFLSGRVRAGHATIMA
                     "
     gene            2534..>2847
                     /locus_tag="VGP50_02905"
     CDS             2534..>2847
                     /locus_tag="VGP50_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026350.1"
                     /translation="MTIAGEMTAGRRAAPALCAAPMTSTSGSGFVDFRITVCGVAELD
                     SHSAVGVTHVLSILDPGTPDPDAFGAFGEHERLDLRFNDIVDETPGMRVPAPEDVAAL
                     LR"
BASE COUNT          469 a          946 c          930 g          502 t
ORIGIN      
        1 cctcgacggc gcggaatcgc tctcgcaatt cgtgcagcgg ctcatcacgg gacgggcggc
       61 gctcgccccc gaatacaccg aggccgacat ctcccgcgac ttcaagagca acggcacgca
      121 ggagcccgac gacgaggact atcaggcgct ggccaagaat ggcttcgccg attggcggct
      181 cgaggtgggc gggctggtcg accatccctt gcgcctctcg ctcgaggagc tgcgtcggct
      241 tcccgcgcgc acgcagatca cccgccatga ctgcgtcgag ggctggagct gcatcggcaa
      301 atggacgggc gcgaagctcg ggccgttgct cgaccgcgcc ggcgtcaagc ccgaggcgcg
      361 ctatgtcgtc ttctactgcg ccgatgccat ggatgcgacg ggcaccaaat attacgagag
      421 catcgatctc gagagcgcct atcatcccca gaccatcctc gcctatgaga tgaacgatca
      481 ggtcctgccc gtgccccatg gtgcgccgct ccggctccgc gtcgagcgcc agctcggcta
      541 caagatggcg aaatacatca tgcgcatcga tctcacccgc ggcttcgccg acatcgccga
      601 cggcggcggc ggctattggg aagaccaggg ctacgagtgg tatgcgggga tttaggttcg
      661 gttgtcctgt ggcgctgaag gacggcgccc ccggtcatgc ggcgcggcaa aatcgagcac
      721 cgggcctttt ggcacgatgc gtgtcgggtt gatcgccgtc atgctcatgt aatagtgccg
      781 cttgatgtga tcgaggctca cggtgtccgc cacgcccggc acctggtaga gctcgcgcag
      841 ataattcgcg aggttcggat agtccgtgat ccgacgcaga ttgcatttga aatggccgta
      901 atagaccgca tcgaagcgga tgagcgtggt gaagagtcgc caatcggcct ccgtcatgtg
      961 ctcgcccacg acataacgct ggtgcgcaag gcgctcttcg agccagtcga gcgccgcaaa
     1021 gacgtcgtcc accgcctgct catagacctc ctgccacgtg gcgaagccgg cgcgatagac
     1081 gccgtaattc acgtctttga agatgcgctc gttcgcgcgg tcgatcgcgg cgcgcagcgc
     1141 cggtggataa tagtcggagc gatccgccgt gagcgcgtcg aaggccgaat tcagcatgcg
     1201 gatgatttcc ggcgattcgt tgttgacgat cgtctgccgc ttcttgtccc agagcaccgg
     1261 cacggagacg cggccggtat agcgcggatt ggccaggaga tagatctcgt agagccggtg
     1321 cttgccgttc agggcatcgg cggtggtgcc ggcgccgaat tcccagcctt gctcgcccat
     1381 caccggctcg accagcgaga gcgagaccac gtcctcgagg cgcttcagct tgcggaagat
     1441 gatggtgcga tgggcaaagg ggcaggcgag cgagacgtag aggtgatagc ggccgcgctc
     1501 ggcggcgaag ccgtcgccgc ccgaaggccc gacgccgcca tcgggcgtga cccaattccg
     1561 ataaagcggc gccgccggca cgaagcgccc gccgctcgcg gaggtgtcgt accattcgcc
     1621 ttgccacacg ccgtcgacga gcacgccatg cgccatggtg atgatccccg gtgccgcccg
     1681 atcagacggg acgctctgtc gctatatagc ccggtcacac gagtgccgtc acggggcgct
     1741 tgaccactcc cggcgacgcc gtcaatgctt tgcgcttggc agccacgccg ggaggatatt
     1801 tcaatgggcg acgcgacgac cgatctggtg ctggcgatcg cgcatcacct gctggtgttc
     1861 atgcttgccg gcgttctcgc cttcgagatc ggcaccgtga ccccgaccat gaagcgcgac
     1921 gaaatcctcg gcgtcggccg tgtcgacaac ggctacggca tccttgccgc ggggatcatc
     1981 gtcgtcggct tcatccgggc caccgtggcg gccaaaggct gggcctatta cgcggtcaac
     2041 ctgttcttct gggcgaagat cgcgaccttc gccgtcgtcg gcctgctctc gatcgtcccg
     2101 acggtggcga tcattcgctg gcgcagggcg ctgaaaagcg atgccgcttt tgcgccgccg
     2161 gccgacgcca tccgtcgcgt ccgcgtctac ctctgggcgg aagcggccct cttcctcttg
     2221 ctcccggtgt tcgccgccgc catggcacgc ggctatggcg aactggtgcc gtgataccga
     2281 aacgtccggt aaagagcccc taaaccaaag tatcgggtgc cttcgttttt ctcctaggtg
     2341 gcttgcccga gggccaatcg atcgattgcc ttgctcccgc tagttttgcc cgggcggcgc
     2401 cggtgaaggg cgacgccgag atcctgcgga gcgcaccgag acgccgcgcc gccgcccgca
     2461 gcggcgcagc gcccgagcgg atcgagacat ttctgtccgg ccgggtgcgg gccggccacg
     2521 ccacgatcat ggcgtgacga tcgcgggcga aatgaccgct ggacggcgcg ccgcgcctgc
     2581 gctatgcgcc gcgccgatga cctcgaccag cggcagcggc ttcgtcgatt tccgcatcac
     2641 cgtctgcggt gtcgcggagc tcgacagcca cagcgcggtc ggcgtgaccc atgtgttgtc
     2701 gatcctcgat ccgggcacgc ccgatcccga cgccttcggc gcgttcggcg agcatgagcg
     2761 cctcgatctc cgcttcaacg acatcgtcga cgagaccccc ggcatgcggg ttcccgcgcc
     2821 cgaggacgtg gcggcgctcc tgcgctt
//
LOCUS       DASYNS010000092        10315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2675220, whole genome shotgun sequence.
ACCESSION   DASYNS010000092 DASYNS010000000
VERSION     DASYNS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2675220"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VGP50_02910"
     CDS             <1..297
                     /locus_tag="VGP50_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEV8026351.1"
                     /translation="KITVLYGAPKDPAAFEKYYADHHMPMVYAVKGIRHVELATGLPQ
                     PDGKPPAFYRITELWFSSAKKMASVTGTPAWKKIIDDVPNFATGGATVLVSQIG"
     gene            360..1343
                     /locus_tag="VGP50_02915"
     CDS             360..1343
                     /locus_tag="VGP50_02915"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEV8026352.1"
                     /translation="MAGPEGTERKLTMSDIRAAAERLTNWGRWGADDEIGTLNFTTPA
                     DIVAAARLVQKGKVMSLALAYDAKGPQGGKSKYPALGRFNPIHVMLRTGTDAYSGVLD
                     HRKIRGADDIIIMPLQCGTQWDGLGHIFYGDHMWNGYDCRTVTSAGAQKCGIEQTRER
                     MVGRGVLLDIPRMLGQEHLADGFAVTAELLDKAERHCRVEVRSGDFLLVRTGQMEAKL
                     ADENWDGYAGGDAPGLAFETLDWLHQKQVAAIVTDTWGVEVRPNQTDEANQPWHWIAI
                     PMMGLTVGEIFYLAELAKDCAEDGRYEFMFVAPALPITGAVGSPANPLAIK"
     gene            1481..1822
                     /locus_tag="VGP50_02920"
     CDS             1481..1822
                     /locus_tag="VGP50_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026353.1"
                     /translation="MPRPQHSSDTDRAEPKLAAPGPRSVAEPLPLRRTASAHGRDPAD
                     PALRSPQERRVTGLTAGERLTVASAITGGDEVRRRRPGMITLFLVVVLIVFAGIGAAS
                     VYHSVAGAFPH"
     gene            1902..2744
                     /gene="otnI"
                     /locus_tag="VGP50_02925"
     CDS             1902..2744
                     /gene="otnI"
                     /locus_tag="VGP50_02925"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEV8026354.1"
                     /translation="MPKLAANLSFLFQELPFLDRFGAAAEAGFRGVECLFPYDTPAAA
                     IAEQLERHRLAQVLLNLPPGDMAAGERGLAALPGRAADFMTALDTALAYARATGCRRL
                     HALSGVWPADRPKEEGEAALVANLRRAADRVAADGITLLIEPINPRDVPGYFLNTTRQ
                     ALAILDRVARDNVKLQLDLYHCQIMEGDLATRLGHLAGRYAHIQIAGVPDRHEPDSGE
                     VNYAYLLDLLDTLGYDGWVGCEYRPAAGTVAGLGWARRWGVGSQPLSRTAGEGGTQRE
                     ALGG"
     gene            2857..4008
                     /locus_tag="VGP50_02930"
     CDS             2857..4008
                     /locus_tag="VGP50_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEV8026355.1"
                     /translation="MAQPLLLSPLSLRSVSLRNRIVISPLCQYSAVEGVANDWHFAHL
                     ARFALGGAGMVFVEATAVHRDGRITHGDMGLWSDEQVPPLRRIAAFLRSQGAVPAIQL
                     AHAGRKASMQRPWHGNGPLDDSDRARGETAWRIVAPSPLPMDEGWLMPVELGRAEMAM
                     LREQWRVAALRAIDAGFEVVELHSAHGYLLNTFLSPLGNRRNDAYGGDRAGRMRFPLE
                     IAETLRAAWPKDRPVFVRISSVDGVDDGWTLEDSIAYAQELKVRGIDVIDCSSGGIAG
                     SATAQRIPRRPGFQVPFAERIRREAEIKTMAVGLILEPRQAEDILEKGQADLIAIGRQ
                     ALFDPNWPLHAEQALAGEAADFAHWPVQAGWWLERREAGLRALRRQRDA"
     gene            complement(4127..5575)
                     /locus_tag="VGP50_02935"
     CDS             complement(4127..5575)
                     /locus_tag="VGP50_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEV8026356.1"
                     /translation="MDLDHDACYRAMCQRDARFDGRFFIAVKTTGVYCRPVCRARTPR
                     SENVIFYPTAAAAQEAGFRPCLRCRPETAPDLGAWRGTSNTVSRALALIEAGALDDAR
                     IDALASRLGVGERQLRRLFREHLGASPVAVAQTRRVLLAKQLIHETRLPMTEIAFAAG
                     FGSIRRFNETFRSLFGRPPGSLRRARGEEISAGPQGEVHLLLRYQPPYDWPLMLAFLE
                     RRAIPGLERVTADGYARTIQLGGVQGVLSVRPGDGNALRAAIRFPKLSALPAIIARLR
                     RVFDLAADPLAIAAHLSQDSWLAPLVAARPGLRVPGAWDGFELAVRAVLGQQITVVGA
                     ARLAGRLVAAYGEPLPAPDGTLTHSFPSPEVVAAADLASLGMPRSRAATLSAVAAALV
                     ADPELLNVTRSLEEAVQKLRAIRGIGEWTAQYIALRQLREPDAFPAADLGLIRALAAA
                     DGTRPAESEVLARAEKWRPWRAYAAQHLWAAA"
     gene            5719..6327
                     /gene="ogt"
                     /locus_tag="VGP50_02940"
     CDS             5719..6327
                     /gene="ogt"
                     /locus_tag="VGP50_02940"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV8026357.1"
                     /translation="MFNHVFHLEHRETPIGRMMIVTDDQDRLRGADWDDYEDRLRLLL
                     RRHYGANAIDWREATRPSAAARALEAYFGGDLRAIETVPTATNGTDFQRAVWAALRQI
                     PVGSTLSYGALAARLGRATAMRAVGLANGANPIPVVVPCHRVIGADGSLTGFGGGLER
                     KRWLLAHEGVAIGDVFESMPAGRPAHPSPALRPSRAERGNRA"
     gene            complement(6251..8134)
                     /locus_tag="VGP50_02945"
     CDS             complement(6251..8134)
                     /locus_tag="VGP50_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8026358.1"
                     /translation="MPGDKRLVPSAGHDDEDEADALHVLIEAGGKILEVRGPDAVAQD
                     IPAGLVAGAPILELIHPEDRDFFDAALSWALAGSSQAARLHVRLQHGGPTPEAVRAHL
                     RPHRPGVARIALSFEKTLLVRAAQAQLRQVLDDAPQGVLVRTAEGRILYVNPALAEML
                     GYTREEFLATENMTHHIHPEDRPLVTARAHARAANEAAPSQYEFRLLHRDGAVLSAET
                     SAVHAIWDGTPVSISWITNITARKRAEEALRRSEGLFVKIFQESPDIVTLSTVTDGRY
                     VDVNETFLRLSGRKRSDVIGRTSLELGIWSDPALRARLIEKLRREGTFLEVAALAVTP
                     GGEARDYSVSGEIVQFEEQELLLVVSHDITEQRRQEELRWRSQKLEALGTLAGGIAHD
                     LNNTLVPVLSLTKLTLKRLPEGSRERQNLSIVLEASERARDLVKQVVAFSRQEEHAEK
                     TVLDPAAVLQTAADMLRASIPATISMELRVEPVPRLLGDAGQLQQVIINLVTNAAQAI
                     GAAMGTVTVALGTPSERPRGVAADGQDLNEWICLSVSDTGCGMDEATQRRIFEPFFTT
                     KGVGLGTGLGLSVVHGIVASHGGHIELSSAPGNGSTFTLYFPALRATDATPATDAPAA
                     RPA"
     gene            8534..>10315
                     /locus_tag="VGP50_02950"
     CDS             8534..>10315
                     /locus_tag="VGP50_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026359.1"
                     /translation="MLTTRFASSAPQPFANDSAGFDYFSTDFHYMNVAAEIRRLLLKQ
                     RGFVLLNGPPPPDGKLLERYLDGSTAADASEAAPFRANLVRCREGWTFGDVVIAYSRE
                     LGLSPDAGAWSILSQVMQESRKGLTRVLILDNAGALDDGGFDELRRFTRVDDPYILPV
                     VLLAGPGFAERLELDLRSVKPSVVGSLPLQHLMPAEVAAFVRYQVEPIGESVEEAFTP
                     GAVATIAEAAEGDPSVVNQLARQHLDAFAGPRAPVAPVEAQLEHENLPVATDRIPAAE
                     VFSPRAAEPLPEPLFARPTDSAATVEIEPLPRPRRRAERLARNLTIAAALLFVGVYGI
                     AFLYSFVPESSSDGAARPAAPVASAAPVLPPPAQAADRSPGQPLAPPEKRVVSVEPRQ
                     PPAPQDAAPSLQAAAQQAASIEPSAAAPQTPAPAAPAPPPAAIAHPEPVPPAPQAVAG
                     AELAAPPPAPAPAPPAPQAVAGAEPAAPPPAAAPATPPPQTLALAEPAAPPPAAQPKP
                     ITRAELQPVPRSAQAAEPPPASDMSALVERGNRLLAAGDIAPAREFFERAVESGDATA
                     SYGLGKSYDPLFLQQIAQQGVAADATKA"
BASE COUNT         1605 a         3589 c         3524 g         1597 t
ORIGIN      
        1 aagattacgg tgctctatgg cgcgcccaag gacccggccg ccttcgagaa atactatgcc
       61 gatcaccaca tgccgatggt ctatgccgtg aaaggcatcc ggcacgtcga gctggcgaca
      121 gggctgccgc agcccgacgg caagccgccg gccttttacc gcatcaccga gttgtggttc
      181 agcagcgcga agaagatggc gagcgtgacg gggacgccgg cgtggaagaa gatcatcgac
      241 gacgtgccga acttcgcgac cggcggcgcc accgtgctgg tatcccagat cggatagtcg
      301 tttccggctc ctagggtcgg ccggtacgga aaaagcgcag ccacaaatag gagaggagca
      361 tggccggacc ggaaggcacc gagcgcaagc tcaccatgag cgacatccgc gcggcggcgg
      421 agcggctcac gaattggggg cgctggggcg ccgatgacga gatcgggacg ctcaacttca
      481 cgacgcccgc cgacatcgtc gccgcggcgc ggctggtgca gaagggcaag gtgatgtcgc
      541 tggccttggc ttacgacgcc aaggggccgc agggtgggaa atcgaaatat cccgccctcg
      601 gccgcttcaa tccgatccat gtcatgctgc gcaccggcac cgatgcctat tcgggagtgc
      661 tcgatcatcg caagatccgc ggcgccgacg acatcatcat catgccgctg caatgcggaa
      721 cgcaatggga tgggctcggc catatcttct atggcgacca tatgtggaac ggctatgact
      781 gccgcaccgt cacctcggcc ggtgcccaga aatgcggcat cgagcagacg cgggagcgca
      841 tggtcggccg cggcgttctg ctcgacattc cgcgcatgct gggtcaggag catctcgccg
      901 acggcttcgc cgtcaccgcg gagcttctcg acaaggccga gcgccattgc cgcgtcgagg
      961 tgcgaagcgg cgactttctc ctggtccgca ccggccagat ggaggcgaag ctcgccgatg
     1021 agaactggga cggctatgcc ggcggcgacg cccccggcct cgccttcgag acgctcgact
     1081 ggctgcacca gaagcaggtg gcggcgatcg tcaccgacac ctggggagtc gaggtgcggc
     1141 ccaaccagac cgacgaggca aaccagccct ggcattggat cgccattccc atgatggggt
     1201 tgaccgtcgg cgagatcttc tatctggccg agctggccaa ggactgcgcc gaggacgggc
     1261 gctacgagtt catgttcgtg gccccggcgc tccccattac cggcgctgtc ggctctccgg
     1321 ccaatccgct cgcgatcaaa tgatcgggag cgggacaaca aaaaggctta acgggcgtta
     1381 actggattgc ggcggacgta aggcaaatcc tgcccaactc tcgacacaag cccgtgtcag
     1441 ggtcgcgtcg cctgagattc aaaccgcatc tttgagagcc atgccccgtc cccagcactc
     1501 ctccgatacc gaccgtgccg agcccaagct cgccgcaccg ggaccgcgct cggtcgccga
     1561 gcctctgccg ctgcggcgca ctgcttccgc ccatggccgt gatcccgcgg accccgcctt
     1621 gcggtccccg caggagcgcc gggttacggg gctcaccgcg ggtgagcgcc tgaccgtggc
     1681 ttcggcgatc accgggggcg acgaggtccg gcgccgccgt cccggcatga tcacgctgtt
     1741 cctggtggtg gtgctgatcg tcttcgccgg catcggtgcc gcgtcggtct atcattccgt
     1801 cgccggcgca tttccgcact gagcctccgc ccgctctgga ccagctccgc ttcgccgggt
     1861 gagaatgatc ccgggtggta agcccgtcct gttcgagaac gatgcccaag ctcgcggcca
     1921 atctgagctt cctgttccag gagctaccct ttctcgaccg cttcggcgcc gcggccgaag
     1981 caggctttcg cggcgtcgaa tgccttttcc cttatgacac gcccgccgcc gccatcgccg
     2041 agcagctcga gcgccatcgc ctggcgcagg tgttgttgaa cctgccgccc ggcgacatgg
     2101 ccgcgggcga gcgcggcctc gccgccttgc ccggccgcgc ggcggatttc atgacggccc
     2161 tcgacaccgc gctggcttac gcgagggcaa ccggatgccg ccggctccat gcgctgtcgg
     2221 gggtgtggcc ggccgatcgg ccgaaagagg aaggcgaggc ggctcttgtc gccaatctcc
     2281 gccgcgccgc cgatcgcgtc gcggccgatg ggatcacgct gctgatcgag ccgatcaatc
     2341 cgcgcgacgt gcccggctat ttcctcaaca ccacgcgcca ggcgctcgcg atcctcgacc
     2401 gcgtcgcgcg cgacaacgtc aagctgcagc tcgacctcta tcactgccag atcatggaag
     2461 gcgacctcgc gacccgtctc ggccatctcg ccggccgcta cgcccacatc cagatcgccg
     2521 gcgttcccga ccgccacgag cccgacagcg gcgaggtcaa ctacgcctat ctcctcgatc
     2581 tcctcgacac gctcggctac gacggctggg tcggctgcga ataccggccc gccgccggca
     2641 cggtcgccgg cctcggttgg gcgcggcgct ggggcgtcgg ctcacagccc ctctcccgca
     2701 ccgcgggaga gggagggacc cagcgcgaag cgctgggagg gtgaggggtt ttctcaggcg
     2761 aggaaaccct tcgccgaccg tcccttgagt cccggcgact ggccttgcgg caagtgtcat
     2821 gctagccatg ccgtccctca tcggagatct cccggcatgg ctcagcctct gctgctctcg
     2881 cccctttcgc tgcgcagcgt gagcttgcgc aaccgcatcg tcatctcgcc gctctgccaa
     2941 tattcggccg tcgagggcgt cgccaatgat tggcatttcg cgcatctcgc gcgtttcgcg
     3001 ctgggcggcg cgggcatggt gttcgtcgag gcgacggcgg tgcatcggga tgggcgcatc
     3061 acccatggcg atatgggcct gtggtcggac gagcaggtgc cgccgctccg gcgcatcgca
     3121 gctttcctta gaagccaagg tgccgtgccg gcgatccagc tcgctcatgc cgggcgcaag
     3181 gcgagcatgc agcggccctg gcacggcaac gggccgctcg acgacagcga tcgcgcgcgc
     3241 ggcgagacgg cctggcgcat cgtcgcgccc agcccgctgc cgatggatga gggctggctc
     3301 atgccggtcg agctcggccg cgccgagatg gcgatgctgc gcgagcagtg gcgcgttgcc
     3361 gcgcttcgcg ccatcgacgc cggcttcgag gtggtcgagc tgcacagcgc ccatggctat
     3421 ctgctcaaca cgtttctctc gcctctcggc aaccggcgca acgacgccta tggcggcgat
     3481 cgcgccgggc gcatgcgttt tccgctggag atcgccgaga ccctgcgcgc ggcctggccc
     3541 aaggatcgcc cggtcttcgt gcgcatctca tcggtcgacg gcgtcgatga cggctggacg
     3601 ctcgaggaca gcatcgccta tgcgcaggag ctcaaagtcc gcggcatcga tgtgatcgac
     3661 tgctcctcgg gcgggattgc cggctcggcg accgcgcagc gcatcccgcg ccgcccgggc
     3721 ttccaggtgc ctttcgcgga gcgcatccgg cgcgaggcgg agatcaagac catggccgtc
     3781 ggcctcatcc tcgagccgcg gcaggcggag gacatcctcg agaaaggcca ggccgatctc
     3841 atcgccatcg gccgtcaggc gctgttcgat cccaactggc cgctccacgc cgagcaggcg
     3901 cttgcgggcg aggcggcgga tttcgcgcat tggccggtgc aggcgggatg gtggctcgag
     3961 cgccgcgagg cgggcttgcg cgccctccgg cgccagcgcg acgcgtagcg cggcgcgacg
     4021 cgcagcgtcg tcgccagcgg gcggcagcgg tttgctcttg caaaccgcga aagctcgcac
     4081 ccccagcgcg acgcgcagcg tcgtccccga ccaagtcaat ttgccgctaa gccgccgccc
     4141 agagatgctg cgcggcatag gcgcgccacg gccgccactt ctcggcgcgc gccaggacct
     4201 cgctctcggc cgggcgcgtc ccatccgccg cggcgagcgc acggatcaat ccgagatcgg
     4261 cggccgggaa ggcgtcgggc tcgcgcagct ggcgcaaggc gatgtattgc gccgtccatt
     4321 cgccgatgcc gcggatggcg cgcagcttct gcaccgcttc ctcgaggctg cgcgtcacat
     4381 tcagcagctc cggatcggcg acgagcgcgg ccgccaccgc agacagcgtc gcggcgcggc
     4441 tccgcggcat gccgagggac gcgaggtcgg ccgcggccac cacttcgggg cttggaaagc
     4501 tgtgcgtcaa cgtgccgtcg ggcgcgggca agggctcgcc ataggccgcg accagccgcc
     4561 cggcgaggcg cgccgcgccg acgacggtga tctgctgtcc cagcacggcg cgtaccgcga
     4621 gctcgaagcc gtcccaggcg ccgggcacgc gcagcccggg ccgtgccgcg accagcggcg
     4681 ccagccatga atcctgggag agatgcgcgg cgatggcgag cggatcggcc gcgagatcga
     4741 agacgcgccg caatcgcgcg atgatcgccg gcaaggccga gagcttggga aagcggatcg
     4801 ccgcgcgcaa cgcgttgccg tcgcccggac ggacggagag tacgccctgc acgccgccca
     4861 gctggatcgt ccgggcatag ccgtccgcgg tgacgcgctc cagcccggga atggcacgcc
     4921 gctcgaggaa ggccagcatc aggggccagt catagggcgg ctgatagcgc agcagcagat
     4981 ggacctcgcc ttgcgggcca gccgaaatct cttcgccacg ggcgcgccgc aagctgccgg
     5041 gcgggcgccc gaacaagctg cggaaggtct cgttgaagcg gcggatgctg ccgaagcccg
     5101 cggcaaacgc gatctcggtc atgggcagcc gcgtctcgtg aatgagctgc ttcgcgagca
     5161 gcacgcgccg cgtctgggcg acggcgaccg gcgacgcgcc cagatgctcg cgaaagaggc
     5221 gccgcagctg ccgctcgccg acgccgaggc ggctggcgag agcgtcgatc cgggcgtcgt
     5281 ccaacgcgcc cgcctcgatc aaggccagcg cgcgcgagac cgtgttcgag gtgcctcgcc
     5341 aggctccaag gtcgggggct gtctccggcc ggcagcgcag gcagggccgg aaccccgctt
     5401 cctgcgcggc ggccgccgtc gggtagaaga tgacattctc ggagcgcggc gtgcgtgccc
     5461 ggcagaccgg gcggcaatag acgccggtgg tcttcacggc gatgaagaaa cgcccgtcga
     5521 agcgggcgtc gcgctggcac atcgcgcgat agcaggcgtc atgatcgagg tccatggcct
     5581 cgatactgag gcacggccgc gaccggggct agcggtttcc ggacatggtc atgtcaggcg
     5641 gtaaccgtgt ccgaaaaccg ccagcaacgg cgcgcgacaa gaccgacata tgaccgaagc
     5701 cccgaagaaa ggattcccat gtttaaccac gtcttccatc tcgagcatcg cgagaccccg
     5761 atcggccgca tgatgatcgt caccgacgac caggatcgcc tgcgcggcgc ggattgggac
     5821 gattacgaag accgcttgcg gcttctgctg cgccgacact acggcgcgaa cgcgatcgac
     5881 tggcgcgagg cgacgcgtcc gtcggcggcg gcacgcgcgc tcgaagcgta tttcggcggc
     5941 gacctgcggg cgatcgagac cgtgccgacc gcgaccaacg gcaccgattt ccagcgggcg
     6001 gtctgggcgg cgctccgtca gatcccggtc ggctccaccc tgagctacgg ggcactggcg
     6061 gcgcgcctcg gccgcgcgac ggccatgcgc gcggtcggcc tcgccaacgg tgccaatccc
     6121 atacccgtcg tcgtcccctg ccatcgggtg atcggcgccg atggctcgct gacgggcttt
     6181 ggcggcgggc tcgagcgcaa acgctggctg ctcgcccatg agggcgtggc gatcggcgac
     6241 gtgttcgagt ctatgccggc cgggcggccg gcgcatccgt cgccggcgtt gcgtccgtcg
     6301 cgcgcagagc ggggaaatag agcgtgaagg tgctgccatt gccgggagcg ctcgacagct
     6361 cgatgtgacc gccatgagag gcgacgatgc catgcacgac cgagaggccg aggccggtgc
     6421 cgaggccaac gcccttcgtc gtgaagaacg gctcgaagat gcgtcgctgc gtcgcttcat
     6481 ccatgccgca gccggtatcg ctcaccgaaa ggcagatcca ttcgttgaga tcctgaccgt
     6541 cggcggcgac gccgcgcggc cgctccgacg gcgttcccag cgcgacggtg acggtgccca
     6601 tggcggcgcc gatcgcctgg gccgcgttgg tgacgagatt gatgatgacc tgctggagct
     6661 gtccggcatc gccgaggagt cgcggcaccg gctcgacgcg aagctccatg ctgatcgttg
     6721 cgggaatgct cgcccgcaac atgtccgcgg ccgtctgcag cacggcggcc ggatcgagga
     6781 ccgtcttctc cgcatgctcc tcctggcggc tgaacgccac gacctgcttg acgagatcgc
     6841 gcgcccgctc gctcgcttcc aggacgatcg acagattctg ccgctcgcgg ctgccctcgg
     6901 gcaggcgctt caaggtgagc ttcgtgagcg acagcaccgg cacgagcgta ttgttgagat
     6961 catgggcgat gccgccggcc aaggtgccga gcgcttcgag cttctgcgag cgccagcgta
     7021 gctcttcctg gcggcgctgc tcggtgatgt cgtgggaaac cacgagcagc agctcttgct
     7081 cctcgaactg cacgatttca cccgagacgg aatagtcgcg cgcctcgccg cccggagtga
     7141 cggcgagcgc ggcgacctcg aggaatgtgc cctcgcggcg gagcttctcg atgaggcgtg
     7201 cgcgcagggc ggggtcgctc cagatgccga gctcgagcga agtgcggccg atcacgtccg
     7261 agcgcttgcg gccggagagc ctgaggaagg tctcgttcac gtcgacatag cgtccgtccg
     7321 tgaccgtgct cagcgtgacg atgtcggggc tttcctggaa gatcttgacg aagagccctt
     7381 cggagcgcct caaggcctcc tcggcgcgct tgcgtgcggt gatgttggtg atccaggaga
     7441 tggagaccgg cgtgccgtcc caaatcgcat gcacggctga ggtctcggcc gagaggacgg
     7501 cgccgtcgcg gtgcagcagg cgaaattcgt attgcgacgg cgctgcctcg ttggcggcgc
     7561 gggcatgggc gcgcgccgtc accagcggcc gatcttccgg atggatatgg tgcgtcatgt
     7621 tctcggtcgc gaggaactcc tcgcgcgtgt agcccagcat ctcggcgaga gcgggattga
     7681 cgtagaggat gcggccctcc gccgtgcgca cgagcacgcc ctgcggcgca tcgtcgagga
     7741 cttggcgaag ctgcgcctgg gccgcgcgca cgagcagcgt cttctcgaag ctgagcgcga
     7801 tccgggccac gccgggccgg tgaggcctga ggtgggcgcg gaccgcttcg ggagtgggtc
     7861 cgccgtgttg cagtcggaca tggagacgcg ccgcctggct cgacccggcc agcgcccagc
     7921 tcagcgctgc atcgaagaag tcgcgatcct cgggatgaat cagctcgaga attggcgcgc
     7981 cggccacgag accggccggg atgtcctgag caacggcgtc ggggccgcgg acctccagga
     8041 tctttccacc ggcctcgatg aggacatgga gcgcgtcggc ctcatcctcg tcatcgtgtc
     8101 cagccgaggg taccagtcgc ttgtcgccgg gcatttcggg gggtcctgtg gacgatattg
     8161 ctcttcgccc ggccgaatgg cccggacgcg gtctgctcca ctgcttccct tgacttcccg
     8221 ccccatgctg gcgctcggcc cgttaacgac gcgctaagaa tggcggaaaa ttcgttggcg
     8281 tcaggggatc cgtgagcact atgaggcacg cccctaagct tcggttcgac aagggaaatc
     8341 gaggccggat ggcagcaaga tcagaacaac tgcgcgctgg ataccgggta gaaaattagg
     8401 taacatgtca tgaaaatctc gaccccggcg aatcgagggc gaggggaggc acagtgcgat
     8461 catttactgc cgccaatctt cggagcggtc gttgcggcgt gcaggcgcgg cgcgcgggcg
     8521 cgggagagcg gttatgctga cgacgcgttt cgcctcatcg gcgccacagc ctttcgccaa
     8581 cgattccgcc ggcttcgatt acttctcgac cgacttccac tacatgaatg tcgccgccga
     8641 gatccgccga ctgcttctca agcagcgcgg cttcgtgctg ctgaacggcc cgcctccgcc
     8701 ggacggcaag ctgctcgaga gatatctcga cgggtctacc gcggccgatg cctccgaagc
     8761 ggcaccgttc cgcgccaatc tcgtccgctg ccgcgaaggg tggaccttcg gcgacgtggt
     8821 catcgcctat agccgcgagc tggggctgag ccccgacgcc ggagcctggt cgatcctgtc
     8881 gcaagtgatg caggaatcgc gcaaaggctt gacgcgtgtc ctgatcctcg acaatgccgg
     8941 ggcgctcgat gatggcggct tcgacgagtt gcgccgcttc accagggtcg acgatcccta
     9001 catccttccg gtcgtcctcc tggcgggtcc gggctttgcc gagcgcctgg agctggacct
     9061 gcggagcgtc aagccgtcgg ttgtcggctc tttgcctctc cagcatctga tgcctgccga
     9121 ggtggcggcc ttcgtccgct accaagtcga gccgatcggc gagtccgtcg aggaagcatt
     9181 cacgccgggc gccgtggcaa ccatcgccga agcagcagag ggcgatccca gcgtggtgaa
     9241 tcaactcgcc cggcagcacc tcgacgcctt cgccggtccc cgcgcgccag tggcgcccgt
     9301 cgaagctcag ctcgagcacg aaaacctgcc cgtcgcgacg gatcggatac cggcggcgga
     9361 ggttttctcg ccccgggccg ccgagcccct gcccgagccc cttttcgcgc ggccgaccga
     9421 ctctgcggca acggtcgaga tcgagcctct gccgcggccg cgccggcggg ccgaacgcct
     9481 ggcgcgcaac ctcacgatcg ccgcggctct gctgttcgtc ggcgtctatg ggatcgcctt
     9541 cctctactcg ttcgttcccg aaagttcatc cgatggagcg gcgcggcctg ccgcccccgt
     9601 ggcgagcgcg gcgccggtgc tgccgccgcc cgcgcaagcg gccgatcgtt ccccgggtca
     9661 gccgctggcg ccccccgaga aacgcgtcgt ttctgtcgag ccgcggcaac cgccggctcc
     9721 acaggatgcg gcgccgtcgc tccaggccgc ggcgcagcag gcggccagca tcgagccctc
     9781 ggctgcggcg ccgcagaccc ccgctccggc cgcgccggca ccaccgcccg ccgctatcgc
     9841 tcacccagag ccggtcccac cggcgccaca ggccgtggct ggcgccgagc ttgccgcacc
     9901 gccgcccgct cctgcgccgg ccccaccggc gccgcaggcc gtggctggcg ccgagcctgc
     9961 cgcaccgcct cctgctgcgg cgccggcgac gccaccgcca cagaccctgg ctctcgccga
    10021 gcccgccgcg ccgccgcctg ctgctcagcc caagcccatc acgcgcgccg agctccagcc
    10081 ggtgccgcgg agcgctcagg ccgccgaacc gcctcctgcc agcgatatga gcgcgctggt
    10141 cgaacgcggc aatcgactgc tggccgccgg cgatatcgcc ccggcccgcg agttcttcga
    10201 acgcgcggtc gaaagcggcg atgccacggc gtcctacggc ctgggcaaga gttacgaccc
    10261 gctgtttttg cagcagattg cacagcaggg cgtggccgcg gacgcgacga aggcg
//
LOCUS       DASYNS010000093         3190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1427076, whole genome shotgun sequence.
ACCESSION   DASYNS010000093 DASYNS010000000
VERSION     DASYNS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427076"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VGP50_02955"
     CDS             complement(<1..461)
                     /locus_tag="VGP50_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEV8026360.1"
                     /translation="MEILAPAFARIRVFNAAVERLATGYRWCEGPVWFGDGRYLVWSD
                     IPNDRMLRWDEETGAVAPFRKPSNFANGNTRDRQGRLVTCEHGGRRVTRTEYDGSITV
                     LCDRFEGKRLNSPNDVVVKSDGSIWFTDPPFGILSDYEGHKAEPELHPNVYR"
     gene            complement(864..980)
                     /locus_tag="VGP50_02960"
                     /pseudo
     CDS             complement(864..980)
                     /locus_tag="VGP50_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
     gene            1413..2615
                     /locus_tag="VGP50_02965"
     CDS             1413..2615
                     /locus_tag="VGP50_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026361.1"
                     /translation="MTSVAPSIVAAEAAYPPWPTGIALRQLYRLPSGLVALAAFAAYW
                     GIPAIIAAWDGTFLAATMPQASSDGMEALLGLEWFGTLILHDPATTGTLNYLGDRSHL
                     LFSILLGLEAGVALEILRRLDKAMTTLRRAQLPTPDGRYPSANYALLRRLANHPLLRV
                     VSLLLAAGIFGVFLYFCRSADYAFWWGSTSHGVAGLAFAAIQFAMVYFATQAIFLVAA
                     GALMLARLFLNGLTLRPFHPDGCNGLAPVGTLIFLLWVFALLLAGAVFVATVLGYLGL
                     ETSRVFWVLAFIATLSVPLAAIAPLAATVIAVNRASRQQLQQLEPALDDLFAESLSAV
                     RRGENADAAGDRLGKLQAMHDVLTGVNVWPFNPKALTAAIGASAIQTLLTLNEIVGSA
                     PFTALLAK"
     gene            complement(2556..>3190)
                     /locus_tag="VGP50_02970"
     CDS             complement(2556..>3190)
                     /locus_tag="VGP50_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026362.1"
                     /translation="TRILLPSLICGLPIGFAFDLLIGRARDVFAYDIAPSWLFFAVNG
                     FFSYGLALATAALFPPAVVRTRAPRWRRVAVIAGLALAGAIAAALFPATALARMVAAG
                     CLVVGAGEVAAALARRSGPLGTLLAADAVPFLRFWRWSASLGLIYEVANAVLPVWRWS
                     LSATLPPWTSELFIVAFGYFVLFHPLALAWQSFGEKRGEGRAADDLVERE"
BASE COUNT          511 a         1169 c         1000 g          510 t
ORIGIN      
        1 cgatagacat tgggatggag ctcgggctcg gccttgtgtc cttcgtaatc gctcaggatg
       61 ccgaagggcg gatcggtgaa ccagatcgat ccgtccgact tgacgacgac gtcgttgggc
      121 gagttgaggc gcttgccctc gaagcggtcg cacagcacgg tgatgctgcc gtcatattcg
      181 gtgcgcgtga cacggcgccc accatgctcg caggtgacga gccttccctg ccggtcgcgc
      241 gtgttgccgt tggcgaaatt cgacggcttg cggaacggcg cgaccgcgcc ggtctcctcg
      301 tcccagcgca gcatgcggtc gttgggaatg tcactccaga cgagatagcg cccgtcgccg
      361 aaccacaccg gcccttcgca ccaacgatag ccggtagcga gccgctcgac cgccgcgttg
      421 aacacgcgga tccgggcgaa ggccggcgcc aggatctcga tcgccggatc gggatagctc
      481 tcggccgccg cccagcgcgc ggcgttcgcg gcggcggcgg cttcggcggc gagcgccgct
      541 gcgccctgct ccttgctctg gccgtggctc acgaaattga cggcgtcgaa gcgcgtcata
      601 ttctgctctt gcaggaaata gacggcgtgg ctctcgcgct cggcgaacat ggcgaccagc
      661 acattggcgc cggtcacttg cccgctgagc gcggactgca catggatcgc cgcccgctgc
      721 agcacgcgct ggaagctcgc cgtgggcttc gcgtcctggc cctcctcctt caccagattg
      781 gcgagctcgg tatcgacata gccagtcaga tcccggcgga gcttctcgag atcgacacgc
      841 gcggcgcgca gcacctggcc cgcatcctcg tcctcgctca gcgccagcag cagatgctcg
      901 agtgtcgcat attcatgacg ccgctcattg gcgagcgcga atgcgcggtg cagcgtcttc
      961 tcgagcgtgc gcgacagcat cggacgggtc ctccgcgggc ttcccggcga aggatagcat
     1021 tgcaccgcgc gatgcgaagc atcgtcgcga gcgccgcgcg acgcgaaagc gtcgtcgcga
     1081 gcgggcgcca gcggttgctc ttgcaaccgc gaaagcccgc cccgcagccg caccgcccca
     1141 ttcacaccag ctatttattc cccctcatta tttactcccc ctcattgtcc attccccctc
     1201 tccccccgca agggcggggg gagaggggta aattttatgg atagagcgga aaacgcggaa
     1261 aatgcggaaa tgaagtgcgt gacgccggtg cgtgagacca aagcgcgcgt cgcgtgagat
     1321 agcgcagctc atgaaatcaa agcccagcgc gccccgctcc gccgcgtgaa gtccctcaca
     1381 gcgccaccgg ccgcccctgc tatgctcccg cgatgacgag cgttgcgccg agcatcgtcg
     1441 cggccgaggc ggcgtatccg ccttggccga ccggcatcgc cttgcggcag ctctatcggc
     1501 tgccgtccgg actcgtcgcg ctggcagcct tcgccgccta ttggggcatt ccggcgatca
     1561 tcgccgcctg ggacggcact ttcctcgccg ccacgatgcc gcaagcgtcg agcgacggca
     1621 tggaggcgct gctcggcctc gaatggttcg gcacgctcat cctccacgat cccgccacca
     1681 ccggcacgct caactatctc ggcgaccgct cgcaccttct cttctccatc ctcctcggcc
     1741 tcgaagccgg cgtcgcgctc gagatcctgc gccgcctcga caaagcgatg acgacgctgc
     1801 gccgcgcgca gctcccgacc ccggacggcc gctatcccag cgccaactac gccctgctgc
     1861 gccggctcgc caatcacccg ttgctccgcg tcgtctcgct gctgctggcg gcgggcattt
     1921 tcggcgtctt cctgtatttc tgcaggagcg ccgattatgc cttctggtgg ggctcgacca
     1981 gccatggcgt cgccgggctc gctttcgccg ccatccagtt cgccatggtc tatttcgcga
     2041 cgcaggcgat cttcctggtg gccgcgggcg cgctgatgct ggcgcggctt ttcctcaacg
     2101 ggctgacgct ccggcccttc caccccgacg gctgcaacgg cctcgccccc gtcggcacgc
     2161 tcatcttcct gctctgggtc ttcgccctgc tgctcgccgg cgcggtcttc gtcgcgaccg
     2221 tgctgggtta tctcggcctc gagacgtcgc gggtcttctg ggtgctcgcc ttcatcgcca
     2281 cgctctcggt gccgctcgcc gcgatcgcgc ctttggccgc gacggtcatc gccgtcaacc
     2341 gcgcctcccg ccagcagctg cagcaactcg agcccgcgct cgacgacctc ttcgccgaaa
     2401 gcctgtccgc cgtccgccgc ggcgagaacg ccgacgcggc cggtgaccgc ctcggcaagc
     2461 tccaggcgat gcatgacgtg ctgacgggcg tcaatgtctg gccgttcaat ccgaaggcac
     2521 tcaccgccgc gatcggcgcc tccgccatcc agacactact cacgctcaac gagatcgtcg
     2581 gcagcgcgcc cttcaccgcg cttctcgcca aatgactgcc acgccagcgc cagcggatgg
     2641 aacagcacga aatagccgaa ggcgacgatg aagagctcgc tggtccaggg cggcagcgtc
     2701 gcgctgagcg accagcgcca caccggcagc acggcattgg cgacctcgta gatcaatccc
     2761 agcgacgcgc tccaccgcca gaaccgcagg aagggcactg cgtccgccgc gaggagcgtg
     2821 ccgagcggcc cgctccgccg cgccagcgcc gccgcgacct cgcctgcacc gacgacgagg
     2881 catccggccg ccaccatccg cgccagcgcc gtcgcgggaa acagcgccgc ggcaatcgcc
     2941 ccggcgagcg caaggccggc aatgacggcg acgcgccgcc agcgtggcgc gcgcgtccgg
     3001 acgacagccg gcggaaagag cgccgccgtc gccagcgcca ggccatagga gaagaacccg
     3061 ttcaccgcga aaaagagcca gctcggcgcg atgtcatacg cgaacacatc gcgcgcgcgg
     3121 ccgatgagca gatcgaaggc gaagccgatg ggcaagccgc agatcaaaga cggcaagaga
     3181 atgcgcgtga
//
LOCUS       DASYNS010000094         9516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_959108, whole genome shotgun sequence.
ACCESSION   DASYNS010000094 DASYNS010000000
VERSION     DASYNS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 9516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9516
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959108"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..1571
                     /gene="lpdA"
                     /locus_tag="VGP50_02975"
     CDS             159..1571
                     /gene="lpdA"
                     /locus_tag="VGP50_02975"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV8026363.1"
                     /translation="MAEQAYDVVVIGGGPGGYVAAIRAAQLGLKTACVDKRGTPGGTC
                     LNIGCIPSKALLQSSEKYSETQHALAQHGIKVSGVDLDLPTMMTRKDKVVGENTRGVE
                     YLFKKYKVDYVKGAARFAAPDRLAITPVDGATVTELRAKSFIIATGSDIVPLPGVDID
                     EKTIVSSTGALSLPTVPKSLIVVGGGYIGLEMGSVWRRLGSAVTVVEFLDRITPGMDA
                     EVSRALQRVLTKQGFAFRLGTKVTAAKRGGEGVTLTLEPAQGGPKEEMTAAVVLVAIG
                     RRPYTEGLGLETVGVVVDNKGRIKVDEGFATNVKGIYAIGDVIAGPMLAHKASEEGVV
                     LAEALAGQRGHVNYEAIPAVIYTWPEVASVGRGEEDLKAAGIAYKIGKFPFSANARAR
                     ANADTEGFVKILADARSDRVLGVHIIGPDAGTMIAEATLAMEYGGSAEDIARTCHAHP
                     TLNEAVKEAALAVEGHPIHI"
     gene            1749..2912
                     /locus_tag="VGP50_02980"
     CDS             1749..2912
                     /locus_tag="VGP50_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8026364.1"
                     /translation="MALLCLTSVLGMLGSSSFSALLPEFQRDWGLSNTEAGWISGIFY
                     GGYVAAVPFLVGSTDRIDPRRIYLISFVIAGAAALGFALLAEGFWSALAWRALAGAGL
                     AGGYMPGLKLLTDRVSGPRQSRYVAFYTAGFSLGTATSFAFTGEIAAWFGWRVAFAAA
                     GVSCLLGFLLVLWLLSPRAPTVSGARRVLDFRPVFRNRAAMAFILGYAGHTWELFALR
                     SWLPAFLVSAASLAGDSAGATEASWLSTAVVLLSTVASIYGAELATRSDRRRIIGRIM
                     TLSVVLSALAGFSVGLPIYAVAALCLAYHMIIMADSAALTGGAVATSPLGERGATLAV
                     HSILGFSGGFFGPLAVGAVLDLAGGSASRLAWGLAFLAMGAGSAAGIAAIRRL"
     gene            complement(2914..3963)
                     /locus_tag="VGP50_02985"
     CDS             complement(2914..3963)
                     /locus_tag="VGP50_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026365.1"
                     /translation="MHGTDSAASLSAANSRVWIALACAVALHAAPSPAWAWGATGHRL
                     ISRLAVETLPEEVPAFLRSADALWPIGELGREADRSKTAGAEHDADRDPGHRINPSDD
                     QTAAGVALTPLTPTREAFDTALRAKGSDQYKMGYLPYAIIEGWQQLKMDFAYWRVNAL
                     GERAGKSAEDREWFAKDKRLREMLTLRDLGYWSHFVADASMPLHVSIHYLGWGNFPNP
                     QGYSTALTLHAHTEGDFVAHHVSEEDMRATLRPYRDCGCSIEERAVAHILASHAQLIP
                     LYELDKRNAFDGAHDEGREFVAERLAAAAAELRDMVVDAWRASPDATVGYPNVKLRDV
                     EAGKVLPIEELKGRD"
     gene            complement(4057..5589)
                     /locus_tag="VGP50_02990"
     CDS             complement(4057..5589)
                     /locus_tag="VGP50_02990"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV8026366.1"
                     /translation="MAEGDAGIHAALEPGSGGDTRHFDVLIIGAGLSGIGAACQLQTQ
                     CPAKSFAILEARDVMGGTWDLFRYPGIRSDSDMFTMGYSFRPWPSEKAISDGPSILKY
                     IKDTAAEHDVDRKIRYGHRVTSAAWSTADALWTVEAEVGPERRPQRFTGNFLYMCSGY
                     YDYDAGYTPGWPEMERFRGRVVHPQHWPEDLAHAGRRVVVIGSGATAVTLVPEMAKTA
                     AHVTMLQRSPTYVVSRPARDAIAARLQRWLPARVAHGLTRWKNVLLTMYFYRLSQRRP
                     AQMKDYIVNEVRKALGPDYDVAKHFTPRYNPWDQRMCLIPDADLFDVIRTGKASVVTD
                     EIEGFTETGIKLRSGEQIDADIIVTATGLKVKMMGGMRLAVDGTPVEVSKRLIYKGMM
                     LNDVPNVAFALGYTNASWTLKCELTSAYVCRLLNHMAAKGHAWCMPHRPESGIVEEPA
                     INFSSGYIQRASGILPKQGSQKPWKLHQNYALDLAALRFATIEDGTIEFGPRAARGSR
                     AA"
     gene            5646..6299
                     /locus_tag="VGP50_02995"
     CDS             5646..6299
                     /locus_tag="VGP50_02995"
                     /inference="COORDINATES: protein motif:HMM:NF025338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8026367.1"
                     /translation="MAARAVATERPKTRMRILAACRRLFNERGPAAVTTAEIAASVGI
                     NEGNLYYHFQRKEQIVEALFAEFEHGFRDVAAAYSPGADDPRHYRDYFAGWFNLMWEW
                     RFFYRDGAAVDRMAPRLRRHLKALADDGQAQVRRVLHGMAAAALIRASPQEIEMLVVN
                     GWIIATYWIDYLRSRHGIAAIRREHIDWGAAQAMGLFLPYLTPRGRAVVELARSATA"
     gene            complement(6381..6722)
                     /locus_tag="VGP50_03000"
     CDS             complement(6381..6722)
                     /locus_tag="VGP50_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV8026368.1"
                     /translation="MARKRSAVARLANTAPVFAALGDATRLRMVARLCRGGPLSTASL
                     TEVADMSRQAIAKHLRVLEGAGLLRSGRAGRERVWELETKRLAEIRRSLEQIAAQWDE
                     ALERLRRLVEE"
     gene            complement(6737..7231)
                     /locus_tag="VGP50_03005"
     CDS             complement(6737..7231)
                     /locus_tag="VGP50_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV8026369.1"
                     /translation="MNTDRIEKKVLLRAPRERVWRAVSDAREFGRWFGVAFDGPFVAG
                     TRLTGKIVPTRVDAEIAKLQEPHEGKAFAWLVDRIEPLRRITFRWHPFAVEPDVDYTK
                     EPMTLIVFELEEISGGTLLTITESGFDQIPLERRAKAFTANEGGWTMQTMLIEKYLAA
                     APKI"
     gene            complement(7467..8570)
                     /locus_tag="VGP50_03010"
     CDS             complement(7467..8570)
                     /locus_tag="VGP50_03010"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEV8026370.1"
                     /translation="MDCCGHALTRRQWVWGSLLASVSAMLAGGAGGYATAQVADAMPA
                     AREVLRRNISVDVHTHGGTTGITSTAPPNDDMAKGMRAGALAAVCLADVPDGPLLGRN
                     AAGVLTSLRPPKPGELYKYHLDRLAWVDEMVAEHGLRRAVTAADLEAAHAAGEPAIIA
                     DIEGLDFLETKLERLDEAHQRGVRHAQLVHYTPNDIGDFQTGAVTHNGLTPFGTEAIR
                     ACHRLGFVCDVAHATEETVKQAVKVATRPLLLSHTAIQGSKAMGATSLAGRQISPDHA
                     RAIAETGGSVGIWHFFPSLDRYVDGLKEMAEIVGVDHVSIGTDQQVAPGSVQDYAKWV
                     ELVAAMLHGGFTPEEAGKIAGGNYLRIFRAAVG"
     gene            complement(9100..>9516)
                     /locus_tag="VGP50_03015"
     CDS             complement(9100..>9516)
                     /locus_tag="VGP50_03015"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026371.1"
                     /translation="AKLNVLLSTTSADGRRFRRAQELRLERAHLPAFPLTWTLMHVLD
                     EQSPMHGYDAARAIAARAQLFVILEARDPTLAAVVHDIRNYAPEDIRFGMRYADAVII
                     AEDGTLVADLTKVGALEPDVGDRLEPGWTEREEASE"
BASE COUNT         1450 a         3304 c         3101 g         1661 t
ORIGIN      
        1 gacggactct acccaattct ggaggagttt tcggggctca ggtcagggag aacagccttc
       61 cgcccaccgc ggtgaggaga aatatgacct taccgctgcc ctcttgagcg cgaggtttgc
      121 gaggagtaga agctgctcta caccaattgg agagcgcgat ggctgagcaa gcgtacgacg
      181 tcgtcgtcat cggcggcggt cccggcggtt atgtcgccgc catccgcgcg gcgcagctcg
      241 gcctcaagac cgcctgcgtc gacaagcgcg gcacgcccgg cggcacctgc ctcaacatcg
      301 gctgcattcc ctcgaaggcg ctgctgcaat cctcggagaa atattccgag acccagcatg
      361 cgctcgccca gcacggcatc aaggtctccg gcgtcgatct cgatctcccg accatgatga
      421 cgcggaagga caaggtcgtc ggcgagaaca cgcgcggcgt cgagtatctc ttcaagaaat
      481 acaaggtcga ctacgtcaag ggcgccgcgc gcttcgccgc gcccgaccgc ctggcgatca
      541 cgccggtcga tggcgcgacg gtgacggagc tccgcgccaa atccttcatc atcgccaccg
      601 gttccgacat cgtgccgctg cccggcgtcg acatcgacga gaagaccatc gtctcctcga
      661 ccggcgcgct gtcgctgccc acggtgccga agagcctcat cgtcgtcggc ggcggctata
      721 tcggcctcga gatgggctcc gtctggcgcc gtctcggcag cgccgtgacc gtcgtcgagt
      781 tcctcgaccg catcacgccc ggcatggacg ccgaggtcag ccgtgccctt cagcgcgtgc
      841 tgacgaagca gggcttcgcc ttccgcctcg gcaccaaagt gacggcggcg aagcgcggcg
      901 gcgagggcgt cacccttacc ctcgaaccgg cgcaaggcgg gcccaaggaa gagatgaccg
      961 ccgcggtggt gctggtggcg atcggccggc gcccgtacac cgaagggctc ggcctcgaga
     1021 ccgtgggcgt cgtcgtcgac aacaagggcc gcatcaaggt cgacgagggc ttcgccacca
     1081 atgtcaaagg catctacgcc atcggcgacg tcatcgccgg gccgatgctc gcgcataagg
     1141 cctcggagga aggcgtcgtc ctcgccgagg cgctggcggg ccagcgcggc cacgtcaatt
     1201 acgaggcgat cccggcggtg atctatactt ggcccgaggt cgcgtcggtg ggccgcggcg
     1261 aggaagacct gaaagccgcc ggcatcgctt ataagatcgg caagttcccg ttctccgcca
     1321 atgcccgcgc ccgcgccaac gccgacacag agggcttcgt caagattctc gccgatgcgc
     1381 gaagcgaccg cgtgctcggc gtccacatca tcggtcccga tgccggcacc atgatcgccg
     1441 aagccacgct cgccatggag tacggtggca gcgccgagga catcgcccgc acctgccacg
     1501 cccatccgac actcaacgag gccgtcaagg aagcggcgct cgcggtcgaa ggccatccga
     1561 tccatatttg aggcgtggag cccgacccca cccctcaccc caaccctctc ccacaagggg
     1621 agagggagat cagcgagccc gtagcaaatt ccctctcccc tttgtgggag agggaggggc
     1681 ccgccgcgca gcagcgggag ggtgaggggt cgctcgccgc cgcgatcgag cgttcgcccg
     1741 cgtttctcgt ggcacttctc tgcctcacct cggttttggg gatgctcggc tcctcgagct
     1801 tctcggcgct gctgccggag tttcagcgcg actggggctt gagcaacacc gaggccggct
     1861 ggatcagcgg catcttctat ggcggctatg tcgcggccgt gccgttcctc gtcggctcga
     1921 ccgaccgcat cgatccgcgc cgcatctatc tcatttcttt cgtcatcgcc ggcgccgccg
     1981 cgctcggctt cgcgcttctc gcggaaggct tctggtccgc cctcgcctgg cgcgcgctgg
     2041 ccggcgccgg tctggccggc ggctacatgc cgggcttgaa gctgctcacg gatcgcgtca
     2101 gcgggccgcg tcagagtcgc tatgtcgcct tctatacggc cggcttcagc cttggcacgg
     2161 cgacctcttt cgcctttacc ggcgagatcg ccgcctggtt cggctggcgc gtcgccttcg
     2221 ctgccgcagg cgtcagctgt ctcctcggat ttctgctcgt gctctggctc ttgtcgccgc
     2281 gcgcgccgac ggtgtcgggc gcgcgccgcg tcctcgattt tcgcccggtg tttcgcaatc
     2341 gcgccgccat ggcgttcatc ctcggctatg ccggccacac ctgggagctc ttcgcgctcc
     2401 gctcctggct ccccgccttt ctcgtcagcg ccgcgtccct cgccggcgac agcgccggcg
     2461 ccaccgaggc gagctggctc agcaccgccg tcgtcctcct cagtactgtc gccagcattt
     2521 acggcgccga gctcgccacg cgctccgacc gccgtcgcat catcggccgc atcatgacgc
     2581 tctcggtggt gctctcagcg ctcgccggct tcagcgtcgg gctgccgatc tacgcggtgg
     2641 ccgcgctctg cctcgcctat cacatgatca tcatggcgga ttccgcggcg ctcaccggcg
     2701 gcgccgtcgc gacatcgccg ctcggcgagc gcggcgccac gctcgccgtg cattcgatcc
     2761 tgggattcag cggcggattc ttcggaccgc tcgcggtcgg tgccgtgctc gacctcgccg
     2821 gcggcagcgc gagccgtctc gcctggggcc tcgccttcct cgccatgggc gccggctccg
     2881 ccgccggcat cgccgccatc cgtcggcttt gagtcaatcg cgccctttga gctcctcgat
     2941 cggcagcacc ttgccggcct ccacgtcgcg cagcttcacg ttgggatagc cgaccgtggc
     3001 gtcggggctg gcgcgccagg cgtcgaccac catgtcgcgc aattccgcgg cggcggcggc
     3061 gaggcgctcc gccacgaatt cgcgtccttc gtcatgcgct ccgtcgaagg cattgcgctt
     3121 gtcgagctca tagagtggga tgagctgcgc atggctcgcc aggatatgcg cgaccgcgcg
     3181 ctcctcgatc gagcagccgc aatcgcggta gggccgcagc gtcgcgcgca tgtcttcttc
     3241 gctcacatgg tgggcgacga aatcgccttc ggtatgggcg tgaagcgtga gcgccgtcga
     3301 gtagccctgc gggttcggga aattgcccca tccgagatag tgaatcgaca catggagcgg
     3361 catgctcgca tcggcgacga agtggctcca atagccgaga tcgcgcagcg tcagcatctc
     3421 gcgcaagcgc ttgtccttgg cgaaccactc acgatcttcc gcgctcttgc ccgcgcgctc
     3481 gcccagcgcg ttgacgcgcc aatacgcgaa atccatcttg agctgctgcc atccttcgat
     3541 aatggcgtag ggcagatagc ccatcttgta ctggtcggat cccttggcgc gcagcgcggt
     3601 gtcgaaggct tcgcgcgtcg gcgtcagcgg cgtcagcgcc acgcccgccg ccgtctggtc
     3661 gtcgcttgga ttgatacggt ggcccggatc gcggtccgca tcgtgctcgg cgccggcggt
     3721 cttcgagcgg tcggcctccc gtccaagctc gccgatcggc cagagggcgt cggcgctgcg
     3781 caggaacgcc ggcacctcct cgggcagagt ctcgaccgcg aggcggctga tcaggcggtg
     3841 gccggtcgcg ccccaggccc aggcgggcga tggcgccgcg tgaagcgcta cagcgcaggc
     3901 aagcgcaatc cagacgcggc tgttcgcggc ggaaagcgag gcggcggagt ccgtgccgtg
     3961 catcgggccg tcccgatggt gatgggagga gaggcgtggg cccggcagag gatagcaggc
     4021 cggcctgccg cgctccagga gccgctcagg cgcagctcag gccgcgcggc tcccgcgggc
     4081 cgcgcgcggc ccgaattcga tcgtgccgtc ctcgatcgtg gcgaagcgca gcgccgcgag
     4141 gtcgagggca taattctgat ggagtttcca cggcttctgc gatccctgct tcggcaggat
     4201 gccgctggcg cgctgaatgt agccggagct gaagttgatc gccggctcct cgacgattcc
     4261 ggactcgggc cggtgcggca tgcaccaggc atggcccttt gccgccatat ggttcaggag
     4321 gcggcagaca taagccgagg tgagctcgca tttgagcgtc caggacgcgt tggtatagcc
     4381 cagcgcgaag gcgacattcg gcacgtcatt gagcatcata cctttgtaga tgagccgctt
     4441 cgacacctcg acaggggtcc cgtcgacggc gagccgcatg ccgcccatca tcttcacctt
     4501 gagccccgtg gccgtgacga tgatgtcggc gtcgatctgc tcgcccgagc gcagcttgat
     4561 cccggtttcg gtgaagccct cgatctcgtc cgtcaccacc gaggccttgc cggtgcggat
     4621 gacgtcgaag agatcggcgt cgggaatgag gcacatccgc tggtcccagg gattgtatcg
     4681 cggcgtgaaa tgcttcgcga cgtcgtagtc ggggccgagc gccttccgca cctcgttgac
     4741 gatgtagtct ttcatctgcg ccggccggcg ctgcgacagg cgatagaaat acatcgtcag
     4801 cagcacgttc ttccagcgcg tcaggccatg ggcgacccgc gccggcagcc agcgttgcag
     4861 gcgggcggcg atcgcgtcgc gcgccggccg cgagacgaca taggtcggcg aacgctgcag
     4921 catggtcacg tgagcggccg tcttggccat ctccggcacc agcgtcaccg ccgtggcgcc
     4981 gctgccgatg acgacgacgc gccgccctgc atgggcaaga tcttccggcc aatgctgcgg
     5041 gtggacgacg cggccgcgga accgctccat ctccggccag cccggcgtgt agcccgcgtc
     5101 atagtcgtaa tagccgctgc acatatagag gaaattgccg gtgaagcgct ggggtctcct
     5161 ctccggccca acctccgcct cgaccgtcca gagcgcgtcg gccgtgctcc aggcggcgct
     5221 ggtcacgcga tggccgtagc ggatcttgcg gtcgacgtcg tgctcggccg cggtgtcctt
     5281 gatgtatttc aggatggacg ggccgtcgct gatcgccttc tcgctcggcc agggccggaa
     5341 gctgtagccc atcgtgaaca tgtcgctgtc ggagcggatg cccggatagc ggaagaggtc
     5401 ccacgtgccg cccatcacgt cccgcgcctc gaggatcgcg aagctcttgg ccgggcactg
     5461 ggtctggagc tgacaggcgg cgccgatgcc ggagagcccg gcgccgatga tcagcacgtc
     5521 gaaatgccgc gtgtctccgc cgctgccggg ctccagagcc gcatggattc ctgcgtcgcc
     5581 ttccgccata attcatccta aggtgttcac cttaaagctg tcaacgatcg catcgaggcc
     5641 cggccatggc ggcacgcgcg gtagcaacgg agcggcccaa gacgcgaatg cgcatcctcg
     5701 cggcctgccg gcgcctcttc aacgaacgcg ggccggccgc cgtgacgacg gccgagatcg
     5761 ctgcttccgt gggcatcaac gagggcaatc tctactacca cttccagcgc aaggagcaga
     5821 tcgtcgaagc gctcttcgcc gagttcgagc acggctttcg cgacgtggcg gcggcctaca
     5881 gccccggcgc cgacgatccc cgtcactacc gcgattattt tgccggctgg ttcaacctca
     5941 tgtgggagtg gcgcttcttc tatcgggacg gcgcggccgt cgaccgcatg gcgccgcgtc
     6001 tgcggcggca tctgaaggca ctcgccgatg acggccaggc ccaggtccgg cgcgtccttc
     6061 acggcatggc cgccgcggcg ctgatccgcg ccagcccgca agagatcgag atgttggtcg
     6121 tcaacggctg gatcatcgcc acctattgga tcgactatct gcgctcgcgc cacggcatcg
     6181 ccgccatccg ccgcgagcac atcgattggg gcgccgcgca agccatgggc ctcttcctgc
     6241 cctacctcac cccccgaggc cgcgccgtcg tcgagctcgc ccgctccgcc accgcatgag
     6301 agcaagggtg aggggctcgc gcgccactcg gccgtccgag gaagatgggt ctgccccccg
     6361 tgcctctgtg gtcaatcttc ttattcttcg acgagacggc gcagccgttc cagcgcctcg
     6421 tcccactgcg ccgcgatctg ctcgagggag cggcggattt cggccaggcg cttcgtctcg
     6481 agctcccaga cccgttcgcg cccggcgcgg ccgctgcgca ggagtccggc gccttcgagg
     6541 acgcggagat gcttcgcgat cgcctgccgc gacatgtcgg cgacctcggt gagactcgcc
     6601 gtcgagagcg ggccgccacg gcagagacgc gcgaccatgc gcaggcgcgt cgcgtcgccg
     6661 agcgcggcga agaccggcgc ggtattggcg agccgtgcga cggcagagcg ttttcgtgcc
     6721 atggtcgctg agcctcttat atcttcgggg cggcggcgag gtatttctcg atcagcatcg
     6781 tctgcatggt ccagccgccc tcattcgcgg tgaaagcctt ggcccggcgc tccagcggaa
     6841 tctgatcgaa gcccgattcg gtgatggtca gcagcgtgcc gccggaaatt tcttcgagct
     6901 cgaacacgat cagcgtcatc ggctccttcg tgtaatcgac atcgggctcg acggcaaagg
     6961 ggtgccagcg gaacgtgatc cgccgcagcg gctcgatgcg gtcgacgagc cacgcgaatg
     7021 ccttgccctc gtgcggctcc tggagcttcg cgatctccgc atcgacgcgc gtcggcacga
     7081 tcttgcccgt caggcgcgtg ccggcgacga aagggccgtc gaaggcgaca ccgaaccagc
     7141 ggccgaactc ccgggcatcg ctcactgcgc gccacacgcg ctcgcgcggc gcccgcaaca
     7201 gcactttctt ctcgatgcga tccgtgttca tgagacaacc tcccggttgc cttatatcat
     7261 cattcataac aaaagacaac ccgcgggttg ccgtttcggg caggcaacgc gggaacgcag
     7321 aggacgcaga ggttacgcgg ggggtgcaga ggatgaatgt tcccccgcgt cctccgcggg
     7381 ccctcggcgt cctctgcgtt cctccttctt tgctgccgcc gcgccctttc tcgatttcgc
     7441 caatcacctg tccgaaatcc agcgcatcag cccacggcgg cccggaagat ccgcagataa
     7501 ttgccgccgg cgatcttgcc ggcctcttcc ggcgtgaagc cgccgtgcag catggcggcg
     7561 acgagctcga cccatttcgc atagtcctgc acgctgcccg gggccacctg ctggtcggtg
     7621 ccgatgctga cgtgatcgac gccgacgatt tcggccatct ccttgagccc gtcgacatag
     7681 cggtcgaggc tcggaaagaa gtgccagatg ccgaccgagc cgccggtctc ggcgatggcg
     7741 cgcgcatgat cggggctgat ctgccgcccg gcgagcgacg ttgctcccat cgctttcgat
     7801 ccctggatcg ccgtatgcga gaggagcaac ggccgggtcg ccaccttgac cgcctgcttc
     7861 accgtctcct cggtcgcatg cgcgacgtcg cagacgaagc ccaggcgatg gcaggcgcgg
     7921 atcgcctcgg tgccgaaagg cgtcaggccg ttgtgggtga cggcgcccgt ctggaagtcg
     7981 ccgatgtcgt tgggcgtgta atgcacgagc tgcgcatgcc ggacgccgcg ctgatgcgct
     8041 tcgtcgagcc gctccagctt ggtttcgagg aagtcgagcc cttcgatgtc ggcgatgatc
     8101 gcgggctcgc cggcggcatg cgccgcttcg agatcggccg cggtgacggc gcgtctcaag
     8161 ccgtgctcgg ccaccatctc gtcgacccag gcgagacggt cgagatgata tttgtagagc
     8221 tcgcccggct tcggcggccg aagcgatgtc agcacgcccg ccgcgttccg gccgagaagg
     8281 gggccgtcgg ggacatcggc gagacacacg gcggcgagcg cgcccgcacg catgcccttc
     8341 gccatgtcgt cattcggcgg cgccgtcgag gtgatgccgg tcgtgccgcc atgggtgtgg
     8401 acatcgacag aaatgttcct gcgcaggacc tcacgcgccg cgggcatggc gtcggcgacc
     8461 tgtgccgtgg cgtacccgcc ggcgccgccg gcgagcatcg cgctcaccga agcgaggagc
     8521 gatccccaca cccattgacg gcgcgtcagc gcatggccgc agcaatccat gatgttctcc
     8581 gtttcgaccg tgccgccacg ttggccattc ttgcgccgga cgtcaattca ccgccgcggg
     8641 agcgcgcgca attcgcttgc accgtgccgc cgccgcggtt agggtggcgc cgtcatgaat
     8701 cgcgcgcgct cgatccgtcg attttggtac cgctggcctt tgccggcggt ctaggttcgc
     8761 gcgcattccc gaaccaaagc cgccctgatc cggcggcttt cgtgttttcg ggatttgcct
     8821 cacgaaattt caccggatcc ggacggcccc ttaaaaggag atccgtcatg gtcgatttcg
     8881 acgtcgtcac cggccccgcc ccgcccctcc gtctcccctg ccgcgagccg ccgcccgtgc
     8941 gcaacgcgcc gcgcggcaat gcaacccgtg caccgacaga tcctgaaata gaccaaccct
     9001 ctcccccacc ggggaagagg gaagggtaag ggcgcatcgc ggactcgctc taaattgcga
     9061 catcggctgt gtcttgagca caggggcggg ccgcggcatc tactccgatg cttcttcccg
     9121 ctccgtccag cccggttcga gacggtcgcc cacatcgggc tccagtgctc cgaccttggt
     9181 caggtccgcg acaagcgtgc cgtcctctgc gatgatcacg gcatccgcat agcgcatgcc
     9241 aaatcgtatg tcttccggcg catagttgcg gatgtcgtgc accacagccg cgagcgtcgg
     9301 gtcgcgcgct tcgaggatca cgaacagttg ggcgcgcgcc gctatcgctc gcgccgcgtc
     9361 atagccgtgc atggggctct gctcgtccag cacatgcatg agtgtccaag tcagaggaaa
     9421 agcgggaagg tgcgcccgct cgagccgcaa ctcctgcgca cgacgaaaac gtctgccgtc
     9481 cgcggacgtc gtggacagga gcacattcag ctttgc
//
LOCUS       DASYNS010000095         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4235507, whole genome shotgun sequence.
ACCESSION   DASYNS010000095 DASYNS010000000
VERSION     DASYNS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4235507"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /gene="hisF"
                     /locus_tag="VGP50_03020"
     CDS             complement(<1..716)
                     /gene="hisF"
                     /locus_tag="VGP50_03020"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEV8026372.1"
                     /translation="MLKMRVIPCLDVKDGRVVKGVNFVDLVDAGDPVEQAKVYDREGA
                     DELCFLDITASAENRDTLYDVVARTAEQCFMPLTVGGGVRTVEDIRRLLLAGADKTSI
                     NTAAVARPDFVREGAEKFGSQCIVVAIDAKATGPGRWEVFTHGGRRATGIEATSWAKR
                     MAALGAGEILLTSMDRDGTKQGFDIPLTRAIADAVTIPVIASGGVGTLDHLVEGIRDG
                     HATAVLAASIFHFGTYRIAEA"
     gene            complement(796..1524)
                     /gene="hisA"
                     /locus_tag="VGP50_03025"
     CDS             complement(796..1524)
                     /gene="hisA"
                     /locus_tag="VGP50_03025"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEV8026373.1"
                     /translation="MILYPAIDLKGGQCVRLLRGEMSAATVFNDDPAGQARRFVAAGC
                     RALHVVDLDGAFAGKPMNQQAVAAIVAAAGGVPVQLGGGIRDRATIERWLERGVARVI
                     LGTAALADPGLVKEACRAHPGSVLVGIDARAGRVAVEGWAKSSSREALDLALEFEDAG
                     VAAIVYTDIDRDGALGGVNVQATAALARRLTTPVIASGGVAALAELRSLKRHEKDGIA
                     GVIVGRALYEERIDLEAALQVLDE"
BASE COUNT          271 a          547 c          538 g          229 t
ORIGIN      
        1 gcctcggcga tgcgataggt accgaaatga aagatcgagg cggcgaggac cgccgtggca
       61 tgtccgtcgc gaatgccctc gaccagatga tcgagggtgc cgacaccacc cgaagcgatc
      121 accggaatgg tcaccgcatc ggcgatggcc cgcgtcaagg ggatgtcgaa gccctgcttc
      181 gtgccgtcgc ggtccatcga ggtcagcagg atctcgccgg cgcccaaggc agccatgcgc
      241 ttcgcccagg acgtggcctc gatgccggtg gcgcgccgcc cgccatgggt gaagacttcc
      301 cagcggccgg ggcccgtcgc cttggcatcg atggcaacga cgatgcactg gctgccgaat
      361 ttctccgcgc cctcacgcac gaaatcgggc cgcgcgacgg ccgccgtgtt gatcgacgtc
      421 ttgtcggcgc cggcgaggag gaggcggcgg atgtcctcga cggtgcgcac gccgccgccg
      481 accgtgagcg gcatgaagca ttgttcggcg gtccgcgcca cgacgtcgta gagcgtgtcc
      541 cggttctccg ccgaggcggt gatgtcgagg aaacacagct cgtccgcgcc ctcgcggtca
      601 tagactttcg cctgttcgac gggatcaccg gcatcgacca ggtcgacgaa attgacgcct
      661 ttgacgacgc gcccgtcctt gacatcgaga cagggtatga cgcgcatctt cagcatgggc
      721 gcacggcgga tccggcgcat gtcgcctcgc gggcgacggc ggggatgggg gaagcaacac
      781 gcgcgatcgc cgccattatt catccagcac ttgaagcgcg gcctctaagt cgatgcgctc
      841 ttcatagagc gcgcggccga cgatcacacc ggcgatgccg tctttctcat ggcgcttcaa
      901 cgagcgcagc tccgccagcg cggcgacgcc ccccgaagcg atgaccggcg tcgtcaaccg
      961 ccgcgccagc gctgccgtcg cctggacatt gacgccgccc agcgcgccgt cgcggtcgat
     1021 gtcggtatag acgatggcgg caacgcccgc atcctcgaat tcgagcgcga gatcgagcgc
     1081 ctcgcgcgaa gaggatttgg cccagccctc cacggccacg cggccggcgc gcgcgtcgat
     1141 gccgacgagc accgagcccg gatgggcccg gcacgcttcc ttaacgagcc ccggatcggc
     1201 aagcgccgcg gtgccgagaa tgacgcgggc gacgccacgc tcgagccagc gctcgatggt
     1261 ggcgcgatca cggatgccgc caccgagctg caccggcacg ccgccggcgg cggcgacgat
     1321 cgcggcgacc gcctgctggt tcatcggctt gccggcgaag gcgccgtcga ggtcgacgac
     1381 atgcaaggcc cggcacccgg cggcgacgaa gcgccgcgcc tggccggccg gatcgtcgtt
     1441 gaagacggtg gccgcgctca tctcgccgcg caacaaccgt acacattgtc cgcccttcag
     1501 atcgatcgcc ggatagagga tcacgagagg cgcttgaaat agaaatgtcc ggggatcggc
     1561 tggccatcga cccgggcata gaccg
//
LOCUS       DASYNS010000096        13121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3923942, whole genome shotgun sequence.
ACCESSION   DASYNS010000096 DASYNS010000000
VERSION     DASYNS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 13121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13121
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3923942"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1287)
                     /locus_tag="VGP50_03030"
     CDS             complement(301..1287)
                     /locus_tag="VGP50_03030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEV8026374.1"
                     /translation="MPKAVVCRKLGTPEDLALEDMAPLVLPPGHVRVAVRAAGVNFVD
                     LLMIAGRYQHKPPLPFVPGIEAAGTIVEVAGDLGARFAVADKVMAQLRQGGYAEEAVV
                     RADQVTLLPPGFSFAEGATFRAAYVTAYHALVDRARLAAGETLLVHGAGGGMGLAAVD
                     VGKLLGATVIAAASCEEKLAVAARRGADHLILYGREPLPGAVKRVTQNAGADVVFDPV
                     GGPVFEESLHAIAWGARLLVIGFTGGIGVAKTNLVLIKGASVIGVRAGEDGRRDPERR
                     ARLDAALSAYAAAGRLRPHISHTLPLEHWADAMHVIGERRAIGRVALVTRPL"
     gene            1894..3840
                     /locus_tag="VGP50_03035"
     CDS             1894..3840
                     /locus_tag="VGP50_03035"
                     /inference="COORDINATES: protein motif:HMM:NF033712.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KedN5 family methylcobalamin-dependent radical
                     SAM C-methyltransferase"
                     /protein_id="HEV8026375.1"
                     /translation="MTSITIVQQGVWHMAKESMPLAAGYLASCIRADERLKHDCDVAI
                     ENFSGTASPLEMAIRLLRDGCPDVIGFSVLGWNFRQFSAVAEAIKQVNPAALIVFGGT
                     HVANQAERVFRQCPQVDIVVNGEGEFAFKDIIAHRAGMTPLDQIAGISYRASDGTVRT
                     TAERARILDLDTIPSPILTGAVPLTRADGAFRYDVALMETNRGCPYQCAFCYWGGAIG
                     QKVRSFSRQRLRAELEAVARAGAATVVLCDANFGMLKQDMEFVEDLIEIRNRYGFPLA
                     LETSWAKNKNATFYDIVKLMKKEGLQTSFTLALQTLDDDTLTLMKRKNMKINDWKDLA
                     AWLSEEGVHAYAELIWGAPGQTRQSFLDGYDELAKYVTRIAAYPLLLLPNTDFTDRRR
                     EHGFVTVRGERDDFEYVLANRTVTIDQNLEMIRFLFWARLLSENLVLRALWPALRATI
                     GMRQSEAILSIADHIEASPSEVAAVLRDAARSSFADPDSLAPALELCYVGSGFDLLIR
                     DWWSSVLRPEIPDEWRTVLDSVVRYDLETRPMADPAHRGFDEAAAIDVGGVKHWEVVR
                     SYDFDVPSFIARAEAPHAPAPHPELREQRVRLRFRQGFGEFARSTNHEVTAHYVAKVE
                     LLAEEGAAPPRSGGRAYGDIGAAG"
     gene            3933..4211
                     /locus_tag="VGP50_03040"
     CDS             3933..4211
                     /locus_tag="VGP50_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026376.1"
                     /translation="MREAIGEINETRSDALPFDKLDSLVLYGESGILDSLQLVNLLMS
                     IEEKIADRIDVAASLTSDRAVSRRVSPFRSTTHLVDFILEEIGRAAAA"
     gene            4208..4924
                     /locus_tag="VGP50_03045"
     CDS             4208..4924
                     /locus_tag="VGP50_03045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8026377.1"
                     /translation="MTRPLLMIILVTGTRTGIGRWLAEHLLRGGHTVLGCSRRTAGDM
                     PDGYWHYEVDLTDADAVRQMFHTIGKAHHHIDGLVNNAGTSRMSPFMLTDADDIRAIF
                     ALNVFAVMQCSREAVKLLRRSSQDTTAILNLSSVATLLSLEGQLAYAASKAAVEQTTR
                     TLSRELAPMNIRVNTLGLPPIRTALTRSVEKAKIEALIARQAITRPCDFADLAGPVDF
                     FLSDAARFVTGETLYLGGVR"
     gene            4924..6273
                     /locus_tag="VGP50_03050"
     CDS             4924..6273
                     /locus_tag="VGP50_03050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEV8026378.1"
                     /translation="MTPAWLGGMAERGEAAALVEGNAEISYAALADRIGSMMSSLRAD
                     GLGAGDAVALHGDYSSDGISAFFALAALGAVVAPVTSLTPATIETLQTACGVRLLYRA
                     GAEPARTPWPGDVQHPLVASLRERRAAGLILLSSGSTGKPKAILHDLDALLDLRRNSR
                     ANRRLRILLLLMFDHIGGINTLISTLFSGGTAIIAPERSPEAVCRLIEAHRVQVLPGN
                     PTFLNLMLIGRFEERFDLSSLRLITYGTEPMPEQLLRRLRAAFPRVRFLQTFGTTETG
                     IAGTQSASSESTHFKFNDGDYEHRVVDGELQLRSKTQFLGYLNAADAVLTEDGWFRTG
                     DMVELGPDGYLKILGRAKEVINVGGEKVLPLDLESLLLEHPLVADCVVYGLANAITGQ
                     AVCADVRPSRPIDRTELRRELYAFLNGKVARFKMPAQIRLVSEIETSERFKKRRVRT"
     gene            6344..8062
                     /gene="cmlS"
                     /locus_tag="VGP50_03055"
     CDS             6344..8062
                     /gene="cmlS"
                     /locus_tag="VGP50_03055"
                     /inference="COORDINATES: protein motif:HMM:NF033113.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol-biosynthetic FADH2-dependent
                     halogenase CmlS"
                     /protein_id="HEV8026379.1"
                     /translation="MARSNVLIIGGGPAGSVAALTLLRLGHDVTIFEKEVFPRYRIGE
                     SFLPGTMSIFNRLGLQPMIDAAGFTKKPSATFLWGRDQAPWTFSFTTPKSAAEWIFDH
                     AIQVKREEFDHLLLNEAGRRGAKVLEGCAVTKVNLDDPDGVVLETRQGEKTSRVSADY
                     VIDASGAGSIVAKQLGIRRYDAFYRNLAVWSYFDCPDRFTGDLRGTTFSITFEDGWVW
                     MIPLKGDLYSVGVIVDQSKAVDLQAQGTEAFYETTLAKCRRAMEILGDSKRVDSVRVV
                     RDWSYDTRLFSEGRFFLSGDSACFTDPLFSQGVHLATQSAVCAAAAIDRLSRHPDERD
                     RVHDWYGKCYGETYEQYHEFLASFYTFASFTEPDSRFWNRRRIVESDDDRFERRRWFE
                     RLSAKAEGGGGWEISDFRDRASTMIALGRHQRQALSDEFSDGELIPARLGWISRFTKQ
                     LTSIKKLKWNGEQVLLRDYYKVNPQSFVLEPRQILANESGRMMTKYTADARVRTIFAD
                     LLAEPFDYRTLIRRFGDIGLGDTASQMIIRLFEAGLLSGFDASDRPVHIQDRLRFDGV
                     GVEYEV"
     gene            8089..9483
                     /locus_tag="VGP50_03060"
     CDS             8089..9483
                     /locus_tag="VGP50_03060"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV8026380.1"
                     /translation="MHFGTAALFAIFAFTGVVVPYALWRVAWIRTVAPLGMIQLAVAL
                     LSSPLCLGRALPPLPPGIFEPLSWIAVMLFLFVAGGHAQLDRGVAVQRKHLGIAVASL
                     VIPLAVGAVLGQWLRIAHPDFAGPLGGGAVFSFAVAICVAVTALPMLAILVADLDLYS
                     SAVGRAALVVAVMHDMALWLLLAAVLLIARSAEAPLVGVAALFLAVPVAIVAIFIAIR
                     ALAHWLWERTAKTDRAALVSACGLLLCCATISDVFGLHLAIGAFLAGTIAPRDMFRRA
                     AAAIEPISTVVLLPFFFLAAAQTVPASLDVAHLIEATFVVTTVALVAKFLAGAVSARL
                     AGFSAEDSLVLGSLLQCKGLMEIVAVIMLRDAKIVSAEFVASFIVMSLVTTLAAKPLV
                     GCSRALGRLRQSGFPGPRVLMPRSGKLMFESRRPAEAALAPDGRRLDDDISGRAPIFG
                     ARPQQGRHSDGSRG"
     gene            9467..10300
                     /locus_tag="VGP50_03065"
     CDS             9467..10300
                     /locus_tag="VGP50_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV8026381.1"
                     /translation="MALEDDLAIHYARPTLEQKILTALKASGKDPQRLTPDDLAPVDE
                     FHVGGRQATVDLADGMELRPDMTILDIGCGLGGASRYLAHARGCRVSAIDMAPDFLRV
                     AASLAARVGLGARVIYREASALALPFEEATFDGATLMHVAMNIVDKAAMFREVRRVMK
                     PGGVFGVYDIMRVGPGEIAFPVPWATNASTSFVGTPEEYRRDLQKAGFVVRSERNRRD
                     FGLDYFRALQRRTAEKGVTPLGLHIPMGETAVRQMVNIFGNIERGVVAPVEMICRLER
                     S"
     gene            10502..10951
                     /locus_tag="VGP50_03070"
     CDS             10502..10951
                     /locus_tag="VGP50_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV8026382.1"
                     /translation="MSDIYFEDLRKGQVVELAGVTITESQIIDFALQFDPQPFHIDRE
                     AAARSPMGGLVASGFHTLALSFRLFVDQGLLRQCNAGGAGLNEVRWIKPVRPGDTIRG
                     RVTVLDARESPTSREHGIGRFLFEMVNQTGEPVLSFLGTVLLKRRPR"
     gene            complement(10969..12222)
                     /locus_tag="VGP50_03075"
     CDS             complement(10969..12222)
                     /locus_tag="VGP50_03075"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEV8026383.1"
                     /translation="MTLPRPEHFEPAAAARGAVSKIAAPASASETTPLWVAAGLAMAP
                     AVSMALGRFAYSLLLPGMRADLGWTYTEAGAMNTVSGVGFLIGALIAARVSGWLGAKR
                     ALMLGGLATAATLFCSGLTDRFGLLLALRTVGGTTGAVSFIAGSSLAATAGRGGGAGR
                     APLVLGIYFGGGGFGVVISALVIPPLLASGGWRAGWFALGILSFIAMALAAPSLLRAP
                     LPAGTAPSEAKNGWSLRSLAPQMVSYGLYGLGYIACATYIVAYLRSVQGFDAREVSLF
                     WALVGLAAIGGAFAWGPVLARLKAGWGSVATIGVTTFAAAIPVFMNGRGAAYLSATLF
                     GGAFLAAVAAMTACVRRSVPPRAWTEVIGVLTIGFALGQSIGPVVSGLVSDGANGIQA
                     GLLLSVFIMVAAVAAAAFQREPAKG"
     gene            complement(12309..12941)
                     /locus_tag="VGP50_03080"
     CDS             complement(12309..12941)
                     /locus_tag="VGP50_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8026384.1"
                     /translation="MAGVRQFDEGEMLAKALDLFWKKGLGGTTMLDLAAATGIQRGSL
                     YNAYHGKETLFLLAFESYAARVLKAVRQAFAEPNPRRALQAFLEVGIANMAKGHPPRG
                     CLTTKTATEPDVADTPVQMRLRALLDEMEAIAAEALSKKGARELLVLTPAQTARVAVT
                     FSRGLAVMERVRRDSRILRQSAQALVGALVRDEDGPGRRRPAERRGRRGA"
BASE COUNT         2168 a         4441 c         4244 g         2268 t
ORIGIN      
        1 cgaaagaccg gttttcctgc atcaggacga cgatgtgctt cacgtcctgg atcgtgccgg
       61 tctcgatctt gggctcgatc gccaaagctc ggcggatcga ttccgggaag gcggtgccga
      121 gggcggcggc gcccaggcct cgaaggatgg tacgacggtc ttgcattggc gcgctcccga
      181 tgggtcggca gggaattgcg aggcaaattg gcgggttatt gttacgcttt attggcgaaa
      241 cgcctgacac ctttgtgaca aaccacagct ttgttgcggg atctcgaggg ggggcgccgc
      301 tcagagcggg cgcgtgacga gagcgacgcg gccgatggcg cgccgttcgc caatgacgtg
      361 catcgcgtcg gcccaatgct cgagcggcag cgtgtgcgag atgtgcgggc ggagtcgccc
      421 ggccgcggca taggcgctca gcgccgcgtc gagccgcgcc cgccgctcgg ggtcgcggcg
      481 gccgtcctcg ccggcgcgaa cgccgatgac ggaggcgccc ttgataagga cgagattggt
      541 tttcgcgacg ccgatcccgc cggtgaagcc gatcaccagg agccgcgcgc cccaggcgat
      601 cgcatgcagg ctctcctcga agaccggccc gccgacggga tcgaagacga cgtcggcgcc
      661 ggcgttctgc gtcacccgct tgaccgcgcc cggcaagggc tcgcggccat aaaggatcaa
      721 atggtcggcg ccgcggcgcg cggcgaccgc gagtttctcc tcgcacgacg cggcggcgat
      781 gaccgtcgcg cccagcagct tgccgacatc caccgccgcc agccccatgc caccgcccgc
      841 gccatgcacc aggagagtct cgcccgcggc gagtcgcgcg cggtcgacca gcgcgtgata
      901 agcggtgacg taggcggcac ggaacgtcgc cccttcggcg aagctgaagc ccggcggcag
      961 cagcgtcacc tgatccgccc tgaccaccgc ctcttccgca tacccgccct ggcgcagctg
     1021 cgccatcact ttgtcggcga ccgcaaagcg cgcgccgagg tcgcccgcga cctcgacgat
     1081 cgtccccgct gcttcgatgc ccggcacgaa gggcagcggc ggcttgtgct ggtagcggcc
     1141 ggcgatcatc agcaaatcga cgaaattgac gccggcggcg cggacggcga cgcggacatg
     1201 gcccggcggc aggacgagcg gcgccatgtc ttcgagtgcg agatcctcgg gggtgccgag
     1261 ctttcggcaa acgacggctt tcggcatcgt caattccgac ctgccgcgac cgacgcgcgt
     1321 gctcttcctg tcgaaaacct gcccatgacc agagacggat gaggcctctg cacaattccc
     1381 ccggatctta gcgagctggt ccttctcgct cgaatcgcgt cgacacatcg atcgtctcgc
     1441 gccgcgctcc gccaatgcga attgcgactc tgctcggcgc gagagcgcgt ccgcgacagt
     1501 gccatttcat cgctcgcgcc gcgaacgcag tgcccacttt tttgtcagtc gcgtccgcca
     1561 tgtattcaat gactggtcgc cgatggcgcg aaatttgcgc gtcgcgtgct atttgcgtcg
     1621 ccgctttcgc gcgttcggcc atgtacccta aaggggtgtt cctcgaccat tgacacaccg
     1681 gcggactcca tgggacgttg acatcatcgt caaacatatg aaggtcacgc agacgacgac
     1741 atctattgcg tgcgcgcgac gacgtcgctg acagcggctc gtcgatctca gagaaatcgt
     1801 gctcaccgcc gctcgccagc gccggattcg gcatggcgcc ggctcgatgc gctgcggcga
     1861 ccgtcgaacg cgcgacaagt caaggagcca tacgtgacgt cgatcacgat cgtgcagcag
     1921 ggcgtctggc atatggcgaa ggaatcgatg ccgcttgccg cggggtatct cgcaagctgc
     1981 atccgcgccg acgaacgcct gaagcacgac tgcgacgtcg ccatcgagaa cttctcgggc
     2041 accgcatcgc cgctcgaaat ggcgatccga ttgctgcgcg acggctgtcc cgacgtgatc
     2101 ggcttctccg ttctcggttg gaacttccgg caattctccg cggtcgccga ggcgatcaag
     2161 caagtcaatc ccgccgcgct cattgtgttc ggcggcacgc atgtcgcaaa ccaggccgag
     2221 cgcgtcttcc ggcaatgccc gcaggtcgac atcgtcgtca atggcgaggg cgaattcgcc
     2281 ttcaaagaca tcatcgccca tcgcgccggc atgacgccgc tcgatcagat tgccggcatt
     2341 tcctaccggg cgagcgacgg caccgtgcgg accaccgccg agcgcgcgcg gatcctcgat
     2401 ctggacacga ttccgtcgcc gatcctcacg ggcgcggtcc cgctcacccg ggccgacggc
     2461 gccttccgct acgacgtcgc gctgatggag accaatcggg ggtgtcctta tcaatgtgcg
     2521 ttctgctact ggggcggcgc catcggccag aaagtgcgca gtttctcgcg gcagcgactg
     2581 cgggcggagc tcgaggccgt ggcgcgcgca ggcgcggcga cggtcgtcct gtgcgacgcc
     2641 aatttcggca tgctgaagca ggacatggag ttcgtcgagg acctgatcga gatacggaac
     2701 agatacggtt ttcctctggc cctcgagacc tcctgggcta agaacaagaa tgccaccttc
     2761 tatgacatcg tgaagctgat gaagaaggaa ggcctccaga cctccttcac gctggcgctc
     2821 cagacgttgg acgacgatac gctcacgctg atgaagcgca agaacatgaa gatcaacgac
     2881 tggaaggatc tggcggcctg gctttcggaa gagggcgtgc atgcttatgc cgagctcatc
     2941 tggggcgcgc ccggccagac ccggcagtcc tttctcgacg gatatgacga gcttgccaaa
     3001 tatgtgacgc gtatcgcggc ctatcccctg ctcctgttgc cgaacaccga tttcacggat
     3061 cggcggcgcg agcatggctt cgtgaccgtc cgcggcgagc gcgacgactt cgaatacgtc
     3121 ttggcgaacc gcaccgtgac catcgatcag aatctcgaga tgattcgctt cctgttctgg
     3181 gcgcggcttc tctcggagaa cctggtgctg cgcgcccttt ggccggcgct gcgcgcgacc
     3241 atcggcatgc gccagtccga agcgatcctg tccatcgccg atcatatcga ggcgtcgccg
     3301 agcgaggtcg ccgccgtgct ccgcgacgcc gcgcggtcgt ccttcgcgga tccggactct
     3361 ctcgctccgg cgctcgagct ctgctacgtc ggcagcggct tcgacctgct gatccgcgac
     3421 tggtggtcga gcgtcttgcg gcccgagatt cccgacgaat ggcgaacggt cctcgactcc
     3481 gtcgtcagat acgatctcga gacgcggccg atggcggatc cggcgcatcg cgggtttgac
     3541 gaagcggcag cgatcgatgt cggtggcgtg aagcattggg aagtggttcg cagctacgac
     3601 ttcgacgttc cgtccttcat cgcgcgggca gaggcccccc acgcccccgc gccccatccc
     3661 gagctgcgcg aacagcgggt gcgactgcgg ttccgccagg gatttggcga gttcgcccgg
     3721 tcgacgaatc atgaagtcac cgcgcactac gtcgccaagg tggagctgct ggcggaagag
     3781 ggcgcggcgc cgccgcggtc cggcgggcgc gcctatggcg atatcggagc ggcgggatag
     3841 aggattgcag ttcttttggc ggtcgaacga gaagaggaat gactgtggac tctgtgacag
     3901 agatgacgac gcgacggcgg atcgaggaca tcgtgcggga agcgatcggc gagatcaacg
     3961 agacccggtc ggacgccctg cccttcgaca agctcgactc cctcgtattg tacggcgagt
     4021 ccggcattct ggactccctc cagctggtca atctgctcat gagcatcgaa gagaagattg
     4081 ccgacaggat cgacgtcgcg gcgtctctga cgtcggaccg cgcggtctcg cgccgtgtca
     4141 gtccgttccg cagcacgacg cacctggtcg actttatcct cgaagagata ggccgagccg
     4201 cggcggcatg acgcggccgc tcctcatgat catccttgtc acagggaccc ggacaggaat
     4261 cgggcggtgg ctcgccgaac atctactgcg gggcggccat acggtgctcg gctgcagccg
     4321 ccgcaccgcc ggcgacatgc ctgacggcta ctggcattac gaggtcgatc tgacggacgc
     4381 cgacgccgtc cgtcagatgt tccacaccat cggcaaggcg caccatcaca tcgacggcct
     4441 ggtgaataat gccggtacat cccgcatgag tccgttcatg ctcaccgacg ccgacgacat
     4501 ccgggcgatc ttcgccctca atgtctttgc cgtcatgcaa tgcagtcgcg aggccgtgaa
     4561 gctgttgcgc cgcagcagcc aggacaccac ggccatcttg aacctctcgt cggtcgccac
     4621 gctgctctcg ctcgagggcc agctcgccta tgccgccagc aaggcggcgg tcgagcagac
     4681 gacgcgaact ctcagtcgcg agctcgcgcc gatgaacatc cgggtgaaca cgctcggctt
     4741 gccgcccata cggaccgcgc tcacgcggtc ggtggagaag gcgaagatcg aggcgctgat
     4801 cgcgcgtcag gccataacgc gcccctgcga cttcgccgac ctcgccggtc ccgtggattt
     4861 ctttctgtcc gacgcggccc ggttcgtgac cggggaaacc ctttatctcg gtggcgtgcg
     4921 ctgatgaccc cggcctggct tggcggcatg gcggagcgcg gcgaggcggc ggcccttgtc
     4981 gaggggaatg ccgagatctc ctatgccgcc ctggcggacc ggatcgggtc gatgatgtcg
     5041 agcctccgcg ccgacggcct cggcgccggc gacgccgtgg cgcttcacgg cgactattcg
     5101 tccgacggca tcagtgcgtt cttcgcgctc gcggcgcttg gcgccgtcgt cgcgccggtg
     5161 accagcctga cccccgccac gatcgagacg ctgcaaaccg cgtgcggggt gcgcctgctt
     5221 taccgcgccg gcgccgagcc ggcgcgcacg ccctggccgg gcgacgtgca gcatccgctc
     5281 gtcgcgtcgc tgcgcgagcg tcgggccgcc ggcttgatcc ttctgtcgag cggcagcacc
     5341 ggcaagccga aggcgatcct tcacgacctc gacgcgctgc tcgatctgcg ccggaattcc
     5401 cgcgccaatc gccgattgcg gatcctgctg ttgctgatgt tcgaccatat cggcggcatc
     5461 aacacgctga tcagcacctt gttttccggc ggaaccgcca tcatcgcgcc cgagcggtcg
     5521 cccgaggcgg tgtgccgcct catcgaggcg catcgggtcc aggtgctccc gggaaatccg
     5581 accttcctca atctgatgct catcggccgc ttcgaggagc gcttcgatct ttcgagcctg
     5641 cgcctcatca cctatggcac ggagccgatg ccggagcagc ttctccgccg cctccgcgcg
     5701 gcctttccgc gcgtgcggtt tctgcagacc ttcggcacga ccgaaacggg catcgccggg
     5761 acgcagagcg cctcttccga gagcacgcat ttcaagttca acgacggcga ctacgagcac
     5821 cgcgtcgtcg acggcgagct gcagctccgc tcgaagacgc agtttctggg ctatctcaac
     5881 gccgccgacg cggttcttac cgaggatggc tggttccgca ccggcgacat ggtcgagctc
     5941 ggccccgacg gctacctcaa gatcctcggc cgcgccaaag aggttatcaa tgtcggcgga
     6001 gagaaggtct tgccgctgga tctcgaatcg ctgctgctcg agcacccgct cgtcgccgat
     6061 tgcgtggtct acggcctggc caacgccatc accggacaag ccgtgtgtgc cgatgtccgg
     6121 ccgagccggc cgatcgaccg gacggagctc aggcgggagc tttacgcgtt tttgaacggc
     6181 aaggtcgccc ggttcaagat gcccgcgcag attcgcctcg tgagcgagat cgagacgtcg
     6241 gagcgcttca agaagcgacg cgtccgaaca tgacgccgcc gggggcaccg tcccgctgat
     6301 cccgccgttc gacggcaatt cgcctcgctc aaggagttag gaaatggctc gcagcaacgt
     6361 tctcatcatc ggtggcggac cggcgggctc ggtggcggcc ctgactttgc ttcggctggg
     6421 ccacgacgtc acgatcttcg agaaggaggt cttcccccgg tatcgaatcg gcgagtcgtt
     6481 tctgcccggc accatgtcga tcttcaaccg cctcggcctg cagccgatga tcgatgcggc
     6541 gggcttcacg aagaagccct cggcgacctt tctctggggg cgcgaccagg cgccttggac
     6601 cttttctttc acgacgccca aaagcgccgc cgagtggatc ttcgaccacg ccattcaggt
     6661 caagcgcgag gaattcgatc atctccttct gaatgaggcc gggagacgcg gtgccaaggt
     6721 gctcgaagga tgcgccgtca ccaaggtcaa tctcgatgat cccgacggcg tcgtcctcga
     6781 gacacgacag ggcgagaaga ccagccgcgt ctcggccgac tacgtgatcg acgcatcggg
     6841 cgcgggcagc atcgtcgcga agcagctcgg cattcgccga tatgacgcct tctaccggaa
     6901 ccttgcggtc tggtcctatt tcgattgccc cgaccggttc accggcgatt tgcgcggaac
     6961 gacattctcc attaccttcg aagacggctg ggtgtggatg attccgctga aaggcgacct
     7021 ctacagcgtc ggcgtcattg tcgatcagag caaggcggtg gacttgcagg cgcaggggac
     7081 cgaggccttc tacgagacga ccctggcgaa atgccgccgc gccatggaga ttttgggtga
     7141 ctcgaaacgc gtcgacagcg tgcgcgtcgt ccgggactgg tcctacgaca cccggctctt
     7201 ctcggaaggc cgtttcttcc tgagcggcga ttccgcctgc ttcaccgatc cgctcttttc
     7261 ccaaggcgtg catctcgcga cgcaatcggc cgtgtgcgcc gccgcggcga tcgaccggtt
     7321 gagccggcat cccgacgagc gggaccgggt ccacgactgg tacggcaagt gctatggcga
     7381 gacctatgag cagtaccacg agttcctcgc gtccttctat accttcgctt cgttcaccga
     7441 gccggactcg cggttctgga accggcggcg gatcgtcgag agcgacgacg atcgcttcga
     7501 gcgccgccgc tggttcgagc gtctctcggc gaaagcggaa ggcggcggcg gctgggagat
     7561 atcggacttc cgcgaccgcg catcgacgat gatcgcgctc ggccggcatc aacgccaggc
     7621 gttgtccgac gaattctccg acggcgaact gatcccggcg aggctgggct ggatcagccg
     7681 cttcaccaag cagctgacga gcatcaagaa gctcaaatgg aacggcgagc aggttctgct
     7741 gcgcgactat tacaaggtga atccacagag cttcgtgctc gagccccggc agatcctggc
     7801 gaatgagagc ggcaggatga tgaccaaata taccgccgac gcgcgcgtgc ggacgatctt
     7861 cgcggatctg ctggccgagc ccttcgatta tcgcaccctc atccgccgct tcggcgacat
     7921 cggtcttggc gacacggcgt cgcagatgat cattcgcctc ttcgaggcgg gcctgctgtc
     7981 gggcttcgat gcctccgaca ggcccgtcca tatccaggac cgccttcgct tcgacggcgt
     8041 cggcgtcgaa tacgaagtct gaggaaggcg cggcggagcg ggcaaatcat gcatttcggg
     8101 acggcggcgc tattcgcgat tttcgccttc acgggggtcg tggtgccgta tgcgctatgg
     8161 cgcgtcgcct ggatccggac cgtggcacct ctcggcatga tccagctcgc ggtggcattg
     8221 ctgtcgagcc cgctctgcct gggtcgcgcc ttgcccccgt tgccgcccgg catattcgaa
     8281 ccgctctcct ggatcgcggt gatgcttttt ttgttcgtcg ccggcggcca tgcgcagctc
     8341 gatcgcggcg ttgccgtcca gcggaagcac ttgggcatcg ccgtggccag cctcgtcata
     8401 ccgctcgccg tcggcgccgt tcttggccaa tggctccgga tcgcccatcc agactttgcc
     8461 ggccctttgg gcggcggcgc ggtcttcagt ttcgccgtcg ccatctgcgt cgcggtcacg
     8521 gcgctgccga tgctggcgat tctcgtcgcg gacctcgatc tctacagctc ggccgtcggg
     8581 cgcgccgccc tggtcgttgc cgtcatgcac gacatggctt tatggctatt gctcgccgcc
     8641 gtcctgctga tcgcaaggtc ggccgaggcg ccgctcgtcg gcgtcgccgc cctgttcctc
     8701 gcggtgccgg tggcgatcgt cgcgattttt atcgccatcc gcgcgctcgc ccactggctc
     8761 tgggagcgaa ccgccaagac cgatcgcgcc gcgctcgtct ctgcatgcgg cctgctgctc
     8821 tgctgcgcga cgatctcgga cgttttcggg ctgcatctcg ccatcggcgc tttcctcgcc
     8881 ggcaccatcg cgccccgcga catgtttcga cgcgccgcgg cggccatcga gcccatcagc
     8941 accgtcgtac tgcttccctt cttcttcctg gcggcggcgc agaccgttcc cgcgagcctg
     9001 gacgtcgcac atcttatcga ggctacgttc gtcgtgacca cggtagcgct cgtcgccaaa
     9061 tttctcgccg gagcggtctc ggcccggctg gccggcttct cagccgaaga cagcctcgtt
     9121 ctcggcagtc tgctccagtg caagggcttg atggagatcg tcgccgtcat catgctccgc
     9181 gacgcgaaga tcgtctcggc cgaattcgtc gcctccttca tcgtcatgtc gctggtgacg
     9241 acgctcgccg ccaagcctct cgtgggctgc tcgcgcgcgc tcggccgcct gcgacagagc
     9301 ggatttcccg ggccgcgcgt gctcatgccg cgttccggca agctcatgtt cgaatcgcgt
     9361 cgcccggcgg aagccgcgct tgcgccggac gggcgacgct tggacgacga catatccggg
     9421 cgcgccccta tcttcggcgc gcgaccccag caagggaggc actccgatgg ctctcgagga
     9481 tgatctggcg atccactatg cgcggccgac gctcgagcaa aaaatcctga cggcgctgaa
     9541 ggcatcgggc aaggatcccc agcgattgac gcccgacgat ctggcgccgg tcgatgaatt
     9601 ccatgtcggc gggcggcagg cgaccgtgga cctggcggac ggtatggagc tccggccgga
     9661 catgacgatc ctcgacatcg ggtgcgggct cggcggagcg tcacgctatc tcgctcatgc
     9721 gcgcggctgc cgggtctccg ccatcgacat ggcgcccgac tttctgcgtg tcgcggcatc
     9781 gcttgcggcg cgtgtcgggc tcggcgctcg cgtgatctat cgggaggcga gcgccctcgc
     9841 cctgcccttc gaggaggcga ccttcgacgg cgcgacgctg atgcatgtgg ccatgaacat
     9901 cgtcgacaaa gcggcgatgt tccgcgaggt aaggcgcgtc atgaagcccg gtggcgtgtt
     9961 cggcgtctac gacatcatgc gcgtcgggcc gggcgagatt gcctttcccg tcccctgggc
    10021 gacgaacgcc agcaccagct tcgtcgggac gcccgaggaa tatcgccgcg atctgcagaa
    10081 ggccggcttc gtcgtccgga gcgagcgcaa ccggcgcgat ttcggcctcg actatttccg
    10141 cgcgctgcag cggaggacgg cggagaaagg ggtgacgccg ctgggcctgc acattcccat
    10201 gggcgagacg gcggtgcgcc agatggtgaa tatcttcggc aacatcgagc gcggcgtggt
    10261 cgcgcccgtc gagatgatct gccggctcga aagaagctag agcggatctc gaacaagtgg
    10321 aatcgacact tcctaacatc gtcattcccg cgaaagcggg aacccagggg ctcccggtac
    10381 gtcgctcccg ctgggtcccg gctttcgcgg ggacgatggc gaaggaacgg tcccacgtta
    10441 acggtatgcg ctctagcaca tctgcccgcg ccccctgcgt tggtgacggc gagaacgact
    10501 catgagcgac atttatttcg aagacttgag gaaaggccag gtggtcgagc tcgcgggcgt
    10561 taccatcacc gagagccaga tcatcgattt cgcgctgcag ttcgatccgc agcccttcca
    10621 catcgaccgc gaagccgccg cgcgctcgcc catgggcggg ctggtggcga gcggctttca
    10681 tacgctcgcc ctcagctttc ggctgttcgt cgatcaggga ctgctgcgcc aatgcaacgc
    10741 cggcggcgcc ggcctcaatg aagtgcgctg gatcaagccc gtgcgcccgg gtgacacgat
    10801 acgcggccgc gtcaccgtgc ttgacgcccg cgaatcgccg acgtcgcgcg agcacgggat
    10861 cggccgcttt ctcttcgaga tggtcaacca gacgggcgag cccgtcctgt ccttcctcgg
    10921 caccgtgctg ctgaaacggc gaccccgctg aacacccgct cgggggcgtc agccctttgc
    10981 cggctcccgc tgaaaggccg ccgcggcgac ggctgcgacc atgatgaaga cggagaggag
    11041 caagcccgcc tggatgccgt tggcgccgtc cgataccaga ccgctcacga ccggtccgat
    11101 gctctggccc agggcaaagc cgatggtgag cacgccgatg acttccgtcc atgcccgcgg
    11161 cggcacggag cgccggacgc aggccgtcat cgccgcgacc gcagccagaa aggcgccgcc
    11221 gaacaaggtc gcggacaagt aggccgcgcc cctgccgttc atgaagaccg ggatcgccgc
    11281 ggcaaaggtc gtgacaccga tggtcgcgac gcttccccag cctgccttga gccgggccag
    11341 gaccggtccc caggcgaagg cgccgccgat cgctgcgagg ccgacgagag cccagaacag
    11401 tgaaacttcg cgcgcatcga agccttggac gctgcgaaga taggcgacga tgtaagtcgc
    11461 acaggcgatg tagccgagcc cgtacaatcc atagctgacc atctgcggcg ccagggaccg
    11521 gagagaccag ccgttctttg cttccgaggg cgccgtgcca gcgggaagcg gcgcgcgcag
    11581 cagcgacggc gccgccagcg ccatggcgat gaaggagaga atgccgaggg cgaaccaacc
    11641 cgcgcgccag ccgccgctcg ccagcagcgg cggaatcacc agcgcggaga tcaccacacc
    11701 gaacccgccg ccgccgaagt agataccgag tacgagcggt gcccggcccg cgccgccgcc
    11761 tcgccccgcc gtcgccgcca ggctgctgcc ggcgatgaac gacacggcgc cggtcgtgcc
    11821 gccgacggtc cgcagcgcca gcaagaggcc gaaccggtcg gtcagccccg aacagaagag
    11881 cgtggccgcg gtggcgaggc ctcccagcat caaggcgcgc ttcgcgccga gccagccgga
    11941 gacgcgcgcg gcgatcagcg cgccgatcag gaagccgaca ccgctcacgg tgttcatcgc
    12001 gccggcctcc gtgtatgtcc agccgagatc ggcccgcatt cccggaagca gcagcgaata
    12061 tgcaaagcgt ccgagcgcca tgctgaccgc cggcgccatg gcgagccccg cggcgaccca
    12121 aagcggcgtc gtctccgatg ccgaggccgg cgcggcgatt ttggacaccg cgccccgagc
    12181 ggccgcggcc ggctcaaaat gctcgggtcg cggaagcgtc atcgcgcgtc tccctctgga
    12241 cggtcgaagc gagccgccgc cggtggccat cggctatttt gacagctatg tcaaactaga
    12301 cagtcctgtc aagcaccgcg gcggccgcga cgctctgcgg gacggcgtcg cccgggaccg
    12361 tcctcgtcac ggaccagcgc tcccaccagg gcctgcgcgc tttgcctcag gattcgcgaa
    12421 tcccgacgga cgcgttccat gaccgcgagg ccgcgcgaga aggtcaccgc cacccgggcg
    12481 gtttgcgccg gggtcagcac gagaagctcg cgcgcgccct tcttggacaa cgcctccgcg
    12541 gcgatcgcct ccatctcgtc gagaagcgcg cgaagccgca tctgcaccgg cgtatcggcc
    12601 acatcgggct cggtcgccgt ctttgtcgtg aggcagccgc gcggcggatg gcccttcgcc
    12661 atgttggcga tgccgacctc gagaaacgcc tgcagcgcac ggcgcggatt aggctcggcg
    12721 aaggcctggc gcaccgcctt caggacccgc gcggcgtagc tctcgaacgc cagcaggaac
    12781 agggtttcct tgccatgata ggcgttgtag agcgagccgc gctgaatccc cgtggcggct
    12841 gcgaggtcca acatcgtcgt gccgcccagg cccttcttcc agaagagatc cagcgccttg
    12901 gccagcattt cgccttcgtc gaactgtcgg actccagcca cgggaccgct ccacaaatca
    12961 ccgatcgctg cagcatcgaa cttgacgcga gtgcgtgcga cgggatcgac cgcaaaaaag
    13021 cactcgtgcc gcctgtcgcc gcttacattg tcttatcgtc gcgagcgccg ctcgactccc
    13081 atggcgacac tatgagttcg atggccgcct cgcgcaacag c
//
LOCUS       DASYNS010000097         3968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_491675, whole genome shotgun sequence.
ACCESSION   DASYNS010000097 DASYNS010000000
VERSION     DASYNS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491675"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VGP50_03085"
     CDS             complement(<1..339)
                     /locus_tag="VGP50_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026385.1"
                     /translation="MLLRAIWLTSVALAALSVAGMLLLIVRRMILARSERKRGQRRDA
                     LMVRIVDYLDGTGDYYGLVRLAGQHPDIAGELIGEMRQLVRGAHREKLSDLVVSLGIV
                     AFNLKRLRSRD"
     gene            complement(343..1758)
                     /locus_tag="VGP50_03090"
     CDS             complement(343..1758)
                     /locus_tag="VGP50_03090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HEV8026386.1"
                     /translation="MCDAASPRSDLKILEQPNPLSCERDLLRWLLCCALALMLALVAA
                     DAARAADPAARHLYDEALVLRQHGDFVGAIAKLEAAAKRAPKDVEILLLLGTLYGFVE
                     RFDDAERVLEHARALAPRNNDVLLAVARIKGFRKNFTGAERDVATVLARDPGNLDALA
                     LAGRLAYYQGALDPAEAKFRAVLAANPNDLEGLLGLGDVLAARGEEAAARRLYERAQA
                     LYPNSPDVASRLAQAGAPPLLWRLDTLASWSHFARRPFIPWYESFNQLTYGLTPATDV
                     HGRVDVSQRFGTFDTYYETGVDHRFSPAFGGYLYAGATPHASFRERWDVLSGGSLRVA
                     KGGDTIGGTVLLLDLKDALYRTGFVYSANPGVQQYFLSGRLWFTAKGINTWAAPGAQY
                     LSGYLLRLDAQVTDSLLVYAGWADAPETDLNVTAEVRSIFTGLAWAQSRRLTWHLDYV
                     HDDRHASYIRDAVIFGPSVKF"
     gene            complement(1775..2143)
                     /locus_tag="VGP50_03095"
     CDS             complement(1775..2143)
                     /locus_tag="VGP50_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8026387.1"
                     /translation="MSSILLVDDDPLLRTLVEHKLRLRGFEVISAESGEEGLKQASAR
                     RPDVIVLDAMLPELDGFEVLQRLQEDAATQSIPVIMLTARKQETDIVSALSKGARDYL
                     VKPFLPEELVMRIRNLLPAP"
     gene            complement(2183..3898)
                     /locus_tag="VGP50_03100"
     CDS             complement(2183..3898)
                     /locus_tag="VGP50_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026388.1"
                     /translation="MTWRDGRWSRAASLATALLAVSLLSPRPAPASVLSLCDGPAVES
                     GAHAISYRAVNGSSRTLRLDAKASAESCASLALPLALSDVLAIALIPPAGDHAPEALV
                     LQGGNDAQGRFVIGQIEAWIGATPAVLPILALGRDLLAAWTPRVFGAEERVSVTQAAG
                     EITLTCRAGEQPAGVFIAEGAALPAIPGLELEIAARGDDGFVFAADESARRRDAPLPL
                     MTLPSAAATRSARAKLPESLDRQSPVTWSVLCPRNAGTLTLARAALAPALEATRIAPP
                     ARSAWAWQPQLWQETPASLLDRLRAQHIDRVYITVPLDEDASVAAPATLAAFIEAAAR
                     QGLGVWAVDGDPHAVLLSERAKFRARSAALAAYNRAHPAAGRLGGVQYDIEPYLVPGF
                     ALDPDRWTAAYIDTLAMLGAAAEMPVEAALPFWFPFEKWGAKLADAVGSIALMDYRTA
                     AEEIERSALPALVWGTTYRRAVHIGLELGPLGDEERRRFRRAESGELWRVAVGDQHAL
                     LLLCAPQKNPMGESYALASRNAMPGSGLTFNGRESMVDAMLPLLVAGFEAWPAYGGIA
                     LHGLF"
BASE COUNT          691 a         1291 c         1419 g          567 t
ORIGIN      
        1 gtcgcgactc ctgagccgct tcaagttgaa cgcgacgatg ccaagcgaga ccaccaggtc
       61 agagagcttc tcgcgatggg cgccgcgcac gagctggcgc atctcgccga tgagctctcc
      121 ggcgatgtcc ggatgctgcc ccgccagcct gacaaggccg taatagtcgc ccgtgccgtc
      181 gagatagtcg acgatgcgca ccatgagcgc gtcccgccgt tgcccgcgct tgcgctcgct
      241 gcgcgcgaga atcatgcggc ggacgatcag caacagcatg ccggcgaccg aaagcgcggc
      301 cagtgccacg gaggtcagcc agatcgcccg caggagcatg gctcagaact tcacgctcgg
      361 gccgaagatg acggcgtcgc ggatatagga ggcgtgacgg tcgtcatgga catagtcgag
      421 atgccaggtg agccgccgcg attgcgccca ggcgaggccg gtgaagatgc tgcgcacctc
      481 ggcggtgaca ttgaggtcgg tctcgggcgc gtcagcccag ccggcgtaga cgagcagcga
      541 atccgtcacc tgcgcatcga ggcgcagcag atagcccgac agatattgcg cgccgggagc
      601 cgcccaggtg ttgatgccct tggcggtgaa ccagagccgc ccgctgagga aatattgctg
      661 cacgcccggg ttcgccgagt agacgaagcc agtgcggtag agcgcatcct tgagatcgag
      721 cagcagcacc gtgccgccga tggtgtcgcc gcccttcgcc acgcgcaggc tgccgcccga
      781 gaggacgtcc cagcgctcgc ggaaggacgc gtgcggcgtc gccccggcgt agagatatcc
      841 gccgaatgcc gggctgaagc ggtgatcgac accggtctcg tagtaggtgt cgaaggtgcc
      901 gaagcgctgg ctgacatcga cgcgtccatg cacgtcggtt gcgggggtga ggccgtaggt
      961 gagctggttg aagctctcat accagggaat gaaggggcgg cgcgcgaagt gactccagga
     1021 cgcgagcgtg tcgaggcgcc agagcagcgg cggcgcgccg gcctgcgcga gccgcgacgc
     1081 cacgtccggc gagttcgggt aaagcgcttg cgcgcgctca tagagccggc gcgccgccgc
     1141 ctcttcgccg cgtgcggcga gcacgtcgcc gaggccgagc agcccttcga gatcgttggg
     1201 attggcggcc agcacggcgc ggaacttcgc ctcggccggg tcgagcgcgc cttggtagta
     1261 agcaagacgg ccggcgagcg ccagcgcatc gagattgccc gggtcccgcg cgagcaccgt
     1321 cgcgacatcg cgctcggcgc cggtgaagtt cttgcgaaag cccttgatcc gcgcgaccgc
     1381 cagcaggaca tcgttgttgc gaggtgccag cgcgcgcgcg tgttcgagca cgcgctcggc
     1441 gtcgtcgaag cgctcgacga agccatagag agtgccgagg agcagcagga tctcgacatc
     1501 tttcggcgcg cgtttcgccg ctgcttcgag cttcgcgatg gcgccgacga aatcgccgtg
     1561 ctggcgcagc accagtgcct cgtcgtaaag atgccgcgcc gccggatcgg cggcgcgcgc
     1621 tgcatcagcg gcaacgagcg cgagcatgag agcgagcgca cagcagagga gccagcgcaa
     1681 gagatccctc tcgcaggaga ggggattggg ctgctcgaga attttcagat cagagcgcgg
     1741 gctcgctgcg tcgcacatgg cgagcctcta gacgtcaggg cgccggcagc agattgcgga
     1801 tgcgcatgac cagttcctcc ggcaggaacg gcttcacgag ataatcgcgg gcacctttgg
     1861 agagggccga gacgatgtcg gtctcctgtt tgcgcgcggt cagcatgatc acggggatcg
     1921 actgcgtggc cgcatcttct tgcaaacgct gcaacacctc gaagccgtcg agttccggca
     1981 gcatggcgtc gaggacgatg acgtcggggc gcctggccga tgcctgcttc aagccttcct
     2041 cgccgctctc ggccgagatg acctcgaagc cgcgcagacg cagcttatgc tcaacaagcg
     2101 tgcgcagcaa cgggtcatca tcgacgagga ggatagagga catggatcaa gttctaggcg
     2161 gaaatggtta aggaataccg ctctaaaaca aaccatggag cgcgatgccg ccgtatgcgg
     2221 gccacgcctc gaagccggcg acgagaagcg gcaacatcgc gtcaaccatg ctttcccgac
     2281 cgttaaacgt gagaccgctg cccggcatcg cattgcgcga ggcgagcgca tagctctcgc
     2341 ccatcggatt cttctgcggc gcgcacagaa gcagcagcgc gtgctggtcg ccgaccgcca
     2401 cgcgccagag ctcgccgctc tcggcgcggc ggaagcggcg gcgctcttcg tcgccgagcg
     2461 gcccgagttc gaggccgata tgcaccgcgc gccggtatgt cgtgccccag acgagggccg
     2521 gcagcgcgga gcgctcgatc tcttcggccg ccgtgcgata atccatgagc gcgatcgagc
     2581 ccaccgcatc ggcgagcttc gcgccccatt tctcgaaggg gaaccagaag ggcagcgcgg
     2641 cctccaccgg catctcggcc gcggcgccaa gcatcgcgag cgtatcgata taggcggcgg
     2701 tccagcggtc gggatcgagc gcaaagcccg gcacgagata aggctcgatg tcgtattgca
     2761 cgccgccgag ccggcccgct gccggatggg cccggttgta ggcggcaagc gccgcgctgc
     2821 gcgcgcggaa cttcgcgcgc tcggacagca ggacggcatg aggatcgccg tcgacggccc
     2881 agacaccgag cccttgccgc gccgccgctt cgatgaaggc agcgagcgtc gccggagcgg
     2941 cgacggacgc gtcctcatcg agcgggacgg tgatgtagac gcggtcgata tgctgggcgc
     3001 gaaggcgatc gaggagcgat gcgggcgtct cctgccagag ctgcggctgc caggcccagg
     3061 ccgagcgcgc cggcggcgcg atccgcgtcg cttcgagcgc gggtgcgagt gcggcgcgtg
     3121 ccagcgtcag cgtaccggca ttgcgcggac acaacaccga ccaggtgacg ggagactggc
     3181 gatcgagcga ttccggcagc ttcgcgcgcg cgctgcgggt cgcggcggcg gacggcagcg
     3241 tcatcagcgg cagcggcgcg tcgcggcggc gcgcgctctc atcggcggcg aagacgaagc
     3301 cgtcatcgcc gcgtgcggcg atctcgagct cgagaccggg gatcgccgga agcgcggccc
     3361 cttcggcgat gaagacgccg gcgggctgct cgccggcgcg acaggtgagc gtgatctcgc
     3421 cggctgcttg cgtcacgctg acgcgttcct cggcgccgaa gacgcgcggc gtccaggcgg
     3481 ccagcagatc gcgtccgagc gccaggatcg gcaagaccgc cggtgttgct ccgatccacg
     3541 cttcgatctg gccgatgacg aaacgtccct gcgcgtcgtt gccgccttgg agcaccagag
     3601 cttccggcgc gtgatcgccg gcgggcggga tcaaggcgat tgccagcacg tcgctcaaag
     3661 caagcggcaa ggcgaggctg gcgcagcttt cggccgatgc cttcgcgtcg aggcgtagcg
     3721 tccggctgct gccgttgaca gcgcgatagg agatggcgtg agcgccggac tcgacggcag
     3781 gaccatcaca aagcgagagg acgctcgccg gagccggccg cggcgacagg agggacaccg
     3841 ctaataacgc ggtcgcaagg ctggcggcgc gcgaccaccg gccatcacgc caggtcatcc
     3901 cgggttccgg gcgcgggcat tcgcccagat ccgctgcacg gtctcggcca gggctttcgg
     3961 ctcaaagg
//
LOCUS       DASYNS010000098         7247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2052017, whole genome shotgun sequence.
ACCESSION   DASYNS010000098 DASYNS010000000
VERSION     DASYNS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7247
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052017"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VGP50_03105"
     CDS             complement(<1..351)
                     /locus_tag="VGP50_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEV8026389.1"
                     /translation="MTDIRSGDWADRYAPARLVPYIRLARLDRPIGIWLLLFPGWWAI
                     ALAAGRWPDWRLMALFAIGAIVMRGAGCTLNDIIDRDFDARVERTRYRPLPSGALSLP
                     EALAFLVLQLVVGAA"
     gene            446..1195
                     /locus_tag="VGP50_03110"
     CDS             446..1195
                     /locus_tag="VGP50_03110"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV8026390.1"
                     /translation="MNAKPATRLYVAPALGAGVSIRLEAAPAHYLNHVLRLAPGDAVA
                     LFNGRDGEWLGRIEGFGKGWGAVTIVEQRRAQGVAPDLWLVFAPIKRARIDFLAEKAT
                     ELGVSDLYPVQTQRTAVGRVNLDRLHANAVEAAEQTERLTVPALHEPQDLAALLARWP
                     ADRRLLLCDESGAAAPIGDVLRAAAAARWGVLVGPEGGFAETELDALRKLAFVCPVSL
                     GPRVLRADTAALAALAVLQALVGDWREPRRR"
     gene            1237..2601
                     /locus_tag="VGP50_03115"
     CDS             1237..2601
                     /locus_tag="VGP50_03115"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEV8026391.1"
                     /translation="MSAPSHSGGAPVTDRRQLAEYLAAGCKPPSAWRIGTEHEKFVFR
                     RADHSPAKYEGPSGIRALLDGLTRFGWSPVLDAGTLVGLSQGEAAISLEPGGQFELSG
                     AAVATLHQTCSEVHEHLRQVSVVAKELDLGLMGLGFHPTARRADVPWMPKGRYAVMRH
                     YMPTKGKLGIDMMLRTCTVQVNLDFESEADMVRKLRMSLALQPVAAALFANSPFVEGK
                     PSGYLSYRCHVWTDVDPDRTGLLPFVFEPGFGFERYVDYMLDVPMYFVYRDGRYIDAA
                     GQSFRDFVAGKLPALPGERPTLSDWNDHLTTVFPEVRLKRYLEMRGADGGPWRRLCAL
                     PALWAGLFYDRTALDGAVDLVADWTQEEREKLRRDVPRLGLAASFRGGTVQDVARVMV
                     RLAREGLSRRAQRDSSGGDESQFLDTLTEIAESGRTPAETLLDHYATRWGRRIDPVFT
                     EEAY"
     gene            complement(2743..4692)
                     /locus_tag="VGP50_03120"
     CDS             complement(2743..4692)
                     /locus_tag="VGP50_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026392.1"
                     /translation="MQKIALVTLIASALLAPAARGCDLATAPSTRWTTMRENGIAWLR
                     SPCGERVFSIGVNVVDGGASGAKLSRPHYDWHAVAPSLDDWVEQTRARLDDWGFNAAG
                     AWSLEPQALRLPTAIDLELGRHAQFHWFDPFDPAMAQRLEDEAKRLTAPYRGTPYRLG
                     YFSDNESGWWGGALFVFYSEKPAASHTKQRWLAELNHFYGGSWARFAHDFVPPAGVAS
                     WDELLRAEAMTRLRPGSNGIAAVRHWTEVVAEHYYDLVARALHAADPDALFLGDRLPI
                     YYDPMAVRAEARHVDVIATNYNVDSPEGWIAPYYFAGLRALSGGKPVLVSEWFYAAHE
                     NRTGNRNNGHLMTVETQSERAAGAAAAAQSFAADPSILGLHWFQYYDYPVGGRADTED
                     YNFGLVDIRDRPYDELVSALGAANRRLPELHAEAVRPARQTLAHVVLPYARIDPARAS
                     LIDWPKPASLLPPLKPSPRDVAFGEAYLAWSEDGLALATIGQDYYDLDLLAYDGAFPL
                     GEAYRLELDVDAGAGPRRFTLYFIPPRVKEKGHDHPLMQALLCEGAPPEHQGGACPAV
                     PGAAALYFGADQPRIAAEALLPWSALGLAAPPADGRLRVELSASAWYHSRWMSLSGLS
                     PSRGSAHPRHWTEMHLGPPPTAARE"
     gene            complement(4736..6169)
                     /gene="tldD"
                     /locus_tag="VGP50_03125"
     CDS             complement(4736..6169)
                     /gene="tldD"
                     /locus_tag="VGP50_03125"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEV8026393.1"
                     /translation="MADLAQTDDLFFTRTGMDKERVQEIVGAALHRMEDGELFLEYSQ
                     SESVAFDDGRIKSAAFDTAQGFGLRALSGEATGYAHASELSEDAIRRAGETVRTVHQG
                     HGGILAAPPIGTNQSLYTDANPLGQVAFGEKAKLLAEIDAYARSRDPRVRQVTAVLAG
                     SWQAVQIIRPDGSRAGDIRPLVRLSVSVVVAEGDRMEAGSSGAGGRVPYQHYLDPAQW
                     RAQVDEALREALVNLGSIPAPAGEMTVVLGAGWPGIMLHEAVGHGLEGDFNRKKTSAF
                     AGLIGQRVAAPGVTVVDDGTLKDRRGSLTVDDEGTPSSRSVLIEDGILKGYMQDRLNA
                     RLMGMRPTGNGRRQSYAHAPMPRMTNTYMLAGDKDPQEIIASVKRGLYAVNFGGGQVD
                     ITSGKFVFSASEAYLIENGKVGPAVKGATLIGNGPNALKRVDMIGNDLKLDNGIGTCG
                     KNGQGVPVGVGQPTLRIDGLTVGGTAA"
     gene            6666..>7247
                     /locus_tag="VGP50_03130"
     CDS             6666..>7247
                     /locus_tag="VGP50_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HEV8026394.1"
                     /translation="MELFRRVAAAGATALMALGVLLARMPGALAEEAQDWQFGMQPGV
                     TPVRARIDVLHDYYLLPISAAICVLVLGLLLVVILRFNAKRHPTPSKTTHNPLLEVFW
                     TVVPTLVLIVIAFPSFRLLYYVDHAQKADMTLKITGHQWAWTYEYPDQGLTVEKSLEV
                     DSSLLVPEDQPVKPGQHRLLDVDEPAVLPVGAVV"
BASE COUNT         1122 a         2360 c         2630 g         1135 t
ORIGIN      
        1 ggccgcgccg acgacgagtt gaagcacgag gaaggcaagc gcctccggca ggctgagcgc
       61 gccgcttggc agggggcggt accgcgtgcg ctcgacgcgg gcgtcgaaat cgcggtcgat
      121 gatgtcattc aaggtgcagc cggcgccgcg catgacgatg gcaccgatcg cgaacaacgc
      181 catcagccgc cagtcgggcc agcgcccggc cgcgagcgcg atcgcccacc agcccggaaa
      241 gagcagcagc cagatgccga tgggacgatc gagccgcgcc aggcggatat aggggacgag
      301 ccgcgccggc gcgtagcggt cggcccaatc gccgctgcgg atgtcggtca tgggagggga
      361 ttttgcgggc ggggcgaggg gtgcggcagg ctcggtcatc gcgggccatc ttgctccgcg
      421 cgggcggtgt ggccaagctc gggcgatgaa tgcgaaaccg gcgactcgtc tttatgtcgc
      481 gccggcgctg ggcgcgggtg tctcgatccg actcgaggcg gcgccggcgc attacttgaa
      541 ccatgtgctg cgcctggcgc cgggcgatgc cgtcgcgctc ttcaacggca gggacgggga
      601 gtggctcggg cgaatcgagg gattcggcaa gggctggggc gcggtgacca tcgtcgagca
      661 gcggcgcgcg cagggcgtcg cgcccgatct ctggctcgtc ttcgcgccga tcaagcgcgc
      721 gcgcatcgat ttcctcgcgg aaaaggccac cgagctcggg gtcagcgacc tttatccggt
      781 gcagacgcag cggacggcgg tggggcgcgt caatctcgac cggctgcacg ccaatgccgt
      841 cgaagcggcc gagcagaccg agcggctgac cgtgccggcg ctgcatgagc cgcaggatct
      901 ggcggcgctc ctagcgcgct ggccggcgga ccggcgtctg ctgctctgcg acgagagcgg
      961 ggctgcggcg ccgatcggcg acgtcctgcg cgcggcggcg gcggcgcgct ggggcgtgct
     1021 ggtcggaccg gagggcggct ttgcggaaac ggagcttgac gctttacgca aactcgcctt
     1081 tgtttgcccc gtcagcctcg gaccgcgggt gctgcgcgcc gataccgcgg cgctcgcggc
     1141 gctcgccgtg ctccaggcgc tggtcggcga ctggcgcgag ccgcgccgcc gttaaaccga
     1201 ataagattca ggatcgggga ggtccgcgcc cgcgtcatgt ccgcgccttc acattccggt
     1261 ggcgcaccgg tcaccgaccg gcgccagctc gcggagtatc tcgccgcggg ctgcaagccg
     1321 ccatcggcat ggcgcatcgg caccgagcac gagaaattcg ttttccgccg cgccgaccac
     1381 agccccgcca aatacgaggg cccgagcggc atccgcgcat tgctcgacgg tctcacccgg
     1441 ttcggctggt cgcccgtgct cgatgccggg actctcgtcg gcctctcgca gggcgaggcg
     1501 gccatctcgc tcgagcctgg cgggcagttc gagctgtcgg gcgcggcggt cgcgacgctg
     1561 caccagacct gcagcgaggt gcatgagcat ctgcgccagg tgagcgtcgt cgccaaggag
     1621 ctggacctcg gcctcatggg cctcggattc catccgacgg cgcggcgcgc ggacgtgccc
     1681 tggatgccca agggccgcta cgccgtgatg cggcattaca tgccgaccaa gggcaagctc
     1741 ggcatcgaca tgatgctgcg gacctgcacg gtccaggtga acctcgactt cgagtccgag
     1801 gcggacatgg tgcgcaagct gcgcatgagc ctggcgctcc agcccgtcgc cgcggcgctc
     1861 ttcgccaatt cgcctttcgt cgaaggcaag cccagcggct atctgagcta tcgctgccat
     1921 gtctggaccg atgtcgatcc cgatcgcacc gggctcctgc ccttcgtctt cgagccgggc
     1981 ttcggcttcg agcgctatgt cgactacatg ctcgacgtgc ccatgtattt cgtctaccgc
     2041 gacggccgct acatcgatgc cgcaggccag tccttccgcg acttcgtggc gggcaagctg
     2101 ccggcgctgc caggcgagcg gccgacgctc agcgactgga acgaccacct caccaccgtc
     2161 ttcccggaag tgcgcctgaa gcgctatctc gaaatgcgcg gcgccgatgg cggaccgtgg
     2221 cggcggctct gcgcgctgcc ggcgctgtgg gccgggctct tctatgaccg cacggcgctc
     2281 gacggcgccg tcgatctcgt cgccgactgg acgcaggagg agcgcgagaa actgcgccgg
     2341 gacgtgccgc gcctcgggct cgccgcgtcg ttccgcggcg gcacggtgca ggacgtggcg
     2401 cgagtcatgg tgcggctcgc ccgcgagggc ctcagccgcc gggcgcagcg cgacagcagc
     2461 ggcggggacg agagccagtt cctcgacacg ctgacggaga tcgccgagag cggccggacg
     2521 ccggccgaga cgctgctcga tcactatgcg acgcgctggg gccgccgcat cgacccggtc
     2581 ttcaccgagg aagcctactg acggttcgcg ccccgcgttt tttcgggaac cgccgcacag
     2641 ccctggcgtt agtaaaatgc agcccttgcg gaatggctgc atgacgtgtc tccaagcgcg
     2701 ccccgcatcc tgtcccccca ggtgcggggc gctgcatttg cgtcattcgc gggcggcggt
     2761 ggggggtggg ccgaggtgca tttcggtcca gtgccggggg tgggcggagc cgcgcgacgg
     2821 cgagaggccg ctcagcgaca tccagcgcga atggtaccag gcggaggcgg agagctcgac
     2881 gcgcaggcgc ccgtccgccg gcggcgctgc gaggccgagc gccgaccagg gcagcagcgc
     2941 ctcggcggcg atccgcggct ggtccgcgcc gaagtaaagc gcggcggcgc cgggcaccgc
     3001 ggggcacgcg ccgccctgat gctcgggcgg cgcgccctca cagagaagcg cttgcatgag
     3061 cggatggtcg tggcccttct ccttgacgcg cggcggaatg aagtaaagcg tgaagcggcg
     3121 gggaccggcg ccggcatcga catcgagctc gaggcggtag gcctcgccca gcggaaaggc
     3181 gccgtcatag gcgaggaggt cgaggtcgta ataatcctgg ccgatggtgg cgagggccag
     3241 accgtcctcg ctccaggcaa gataggcttc gccgaacgcg acgtcgcgcg gcgacggctt
     3301 gagcggcggc aggagcgacg ccggcttcgg ccagtcgatg agcgaggcac gcgccgggtc
     3361 gatgcgcgca tagggcagca cgacatgcgc gagcgtctgg cgcgcgggac ggacggcttc
     3421 ggcgtgaagc tcgggcaggc ggcgattggc ggcgccgagc gcgctcacca gctcgtcata
     3481 agggcggtcg cggatgtcga cgaggccgaa attgtaatcc tcggtgtcgg cgcggccgcc
     3541 aacgggatag tcgtaatact ggaaccaatg caggccgagg atgctcggat cggcggcgaa
     3601 gctttgcgcg gcggcggccg caccggccgc gcgctcactt tgcgtctcga cggtcatgag
     3661 atggccgttg ttgcggttgc cggtgcggtt ctcatgcgcc gcatagaacc attccgacac
     3721 gagcaccggc ttgccgcccg agagcgcgcg gaggccggcg aagtaatagg gtgcgatcca
     3781 gccttcgggg ctgtcgacgt tgtagttggt ggcgatgacg tcgacgtggc gcgcctcggc
     3841 cctgacggcc atcgggtcgt agtagatcgg caagcggtcg ccgagaaaga gcgcgtcggg
     3901 atcggcggcg tgcaaggcgc gcgcgacgag atcgtaataa tgctcggcca cgacttcggt
     3961 ccaatggcgt acggcggcga tgccgttgct gcccgggcgt agccgcgtca tcgcctcggc
     4021 gcgcagcagc tcgtcccagg aggcgacgcc ggccggcggc acgaagtcgt gcgcgaaacg
     4081 cgcccagctc ccgccataga agtggttgag ctcggcgagc cagcgctgct tggtatgact
     4141 ggcggcgggc ttctccgaat agaagacgaa gagcgcgccg ccccaccagc ccgattcatt
     4201 gtccgagaaa tatcccaggc gatagggcgt gccgcgataa ggcgccgtga gccgcttcgc
     4261 ttcgtcctcg aggcgctgcg ccatcgcggg atcgaaggga tcgaaccagt ggaattgggc
     4321 atggcggccg agctcgaggt cgatcgccgt cggcagccgc agggcttgcg gctcgagcga
     4381 ccaggcgccg gccgcgttga agccccagtc gtcgagacgc gcgcgcgtct gctccaccca
     4441 gtcgtcgaga ctcggcgcga ccgcgtgcca gtcgtaatgc ggccgcgaca gcttcgctcc
     4501 cgacgcgccg ccatcgacga cgttgacgcc gatcgagaag acgcgctcgc cgcaaggaga
     4561 tctgagccag gcgatgccgt tctcgcgcat cgtcgtccag cgcgtcgacg gggccgtggc
     4621 gaggtcgcag ccccgtgcgg caggagcgag cagcgcggac gcgatcagtg tgacgagagc
     4681 gatcttttgc aaacgtgctg cgaggagagg ggaatgaacg gcgcgacccg ttccctcaag
     4741 ccgcggtgcc accgaccgtg aggccgtcga tgcggagggt gggctggccg acgccgacgg
     4801 ggacgccttg gccgttcttg ccgcaggtgc cgatgccgtt gtcgagcttc aggtcgttgc
     4861 cgatcatgtc gacgcgcttc agcgcattgg ggccgttgcc gatgagggtc gcgcctttga
     4921 ccgccgggcc gaccttgccg ttctcgatga gataggcctc ggacgccgag aacacgaatt
     4981 tgccggacgt gatgtcgacc tggccgccgc cgaaattcac ggcataaagg ccgcgcttca
     5041 ccgaggcgat gatctcctgc ggatccttgt cgcccgcgag catgtaggtg ttggtcatgc
     5101 gcggcatcgg cgcatgggcg tagctctggc gccggccgtt gccggtgggg cgcatgccca
     5161 tgagccgcgc gttgagccgg tcctgcatgt agcctttgag gatcccgtcc tcgatcagca
     5221 cgctgcgcga ggagggcgtg ccttcgtcgt cgacggtgag cgagccgcgc cggtccttga
     5281 gcgtgccgtc atcgaccacg gtgacgcccg gcgccgcgac gcgctggccg atgaggccgg
     5341 caaaggccga ggtcttcttg cggttgaagt cgccttcgag gccatggcca actgcctcat
     5401 gcagcatgat gccgggccag cccgcgccca gcacgaccgt catctcgccg gccggcgcag
     5461 ggatcgagcc cagattgacc agcgcctcgc gcagcgcctc atcgacctgg gcgcgccatt
     5521 gcgcgggatc gaggtagtgc tgataaggga cgcggccgcc ggcgcccgag gagcccgcct
     5581 ccatgcgatc gccctcggcg acgacgacgc tgacgctcaa gcgcaccagc gggcggatgt
     5641 cgccggcgcg gctgccgtcg gggcggatga tctgcaccgc ctgccatgag cccgcgagta
     5701 cggccgtcac ctggcgcacg cgcggatcgc gcgaacgcgc ataggcgtcg atctcggcga
     5761 ggagcttcgc cttctcgccg aaggccactt gccccagcgg attggcgtcg gtgtagaggc
     5821 tctgattggt gccgatgggg ggcgcggcga gaatgccgcc atggccctga tgcaccgtgc
     5881 ggaccgtctc gccggcgcgg cggatcgcgt cctcggagag ctcggacgca tgggcatagc
     5941 cggtggcctc gcccgagagc gcgcgcaggc cgaagccctg cgccgtgtcg aaggcggcgc
     6001 tcttgatgcg cccgtcatcg aaggcgacgc tttccgactg gctgtattcg aggaagagct
     6061 cgccatcctc catgcgatga agggcggcgc cgacgatctc ctggacgcgc tccttgtcca
     6121 tgccggtccg ggtgaagaac aggtcgtcgg tctgggcgag gtcggccatg aacgctcctt
     6181 tgcgcggccc gcttcggggc tgctgcttat tgccatgatg cacccgccgg attctagcga
     6241 aacctcgacc cgggcgcctc aggactatct gaggacgata tggggtgcgg cgagggtagc
     6301 gagaagcggc acgcttggtc ggatccatcc cgacatcctg ctgcggagaa tgcagccgtg
     6361 tgaatcgctg ccgcagtgca gcgacgggac gcaccgcatc aatgtatgcg cgtaagttca
     6421 cttttcataa atgcttagcg caggaatgcg gcataaagtc gcaacgctgc agcattcggc
     6481 gagcgctatg acgacgccac ttgcgacggg acggcggttg tcgatgccgc gcgctttgtt
     6541 gtagggtcgc gccgccttcg cattcgcgaa ggagtcaaag gatgcaaacg gacatgggcg
     6601 accgcgacgc ggaggggcga aggcccggcc ggctcgcgcc tgttcggaat gggagacgac
     6661 ggagcatgga attgtttcgg cgcgttgcgg cagccggcgc cacggcgctg atggcgctcg
     6721 gtgtccttct cgcaaggatg ccgggagcgc tggccgaaga ggcgcaagac tggcagttcg
     6781 gcatgcagcc cggcgtgacg cccgtgcgcg cgcggatcga cgttctgcat gactattatc
     6841 tgttgccgat cagcgcggcg atctgcgttc tcgtgctggg cctgctgctc gtggtgatcc
     6901 tgcgcttcaa cgcgaagcgc catccgacgc cgtcaaagac gacgcacaac ccgctgctcg
     6961 aagtgttctg gacggtggtg ccgaccctgg tgctgatcgt catcgccttc ccgtccttcc
     7021 ggctgctcta ttacgtcgac catgcgcaga aggccgacat gacgctcaag atcacggggc
     7081 atcagtgggc ttggacctat gaatatcctg accagggcct gaccgtcgag aaaagcctgg
     7141 aggtcgacag cagccttttg gtgccggaag accagccggt gaagccgggc caacatcgcc
     7201 tcctcgatgt ggacgagccg gcggtgctgc cggtgggcgc cgtcgtg
//
LOCUS       DASYNS010000099         3317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2520768, whole genome shotgun sequence.
ACCESSION   DASYNS010000099 DASYNS010000000
VERSION     DASYNS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2520768"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VGP50_03135"
     CDS             <1..860
                     /locus_tag="VGP50_03135"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEV8026395.1"
                     /translation="RMFVPYPGQVKIEDLLRGVIVQSGNDACVVLAEGLAGSEAAFVE
                     RMNATAEKLGLTNSHFANVHGLPAPGHYMSPRDLTMLSRHLIQDFPQYYRYEAEKEFT
                     YNGVKQGNRNPLLYRNIGADGIKTGHTEEAGYGLVGSAIRSGRRVIVMLSGMSSMKER
                     DVESEKVLEWAYREFNDYTIGKAGEPIDEAPVWLGAHAKVPAAPAEDLVVTLPRAARR
                     DLKVMAVYDGAVKAPIAKGQTVGKLVVTAPDTDTIETPLVATQAVDRLDALSRVGVGV
                     GYLLLGHKN"
     gene            985..1635
                     /gene="tmk"
                     /locus_tag="VGP50_03140"
     CDS             985..1635
                     /gene="tmk"
                     /locus_tag="VGP50_03140"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV8026396.1"
                     /translation="MTAKHGFFCVFEGVDGSGKTGTLAHIESALAKDGVALLATREPG
                     GTEEGLALRKLLLGGSHRWTPNAELLLVNAARAQHVEKVLRPALGDGKLVLCDRFIGS
                     TLAYQGAGHGIGDERIRALHKIAAGDLWPDLTILLDVDPELAVKRSRRRLAASASDEG
                     RFEALDLDFHRRVRRSFLDQAARAPTAHVVLDASRPQQIVEADALAALRAALKARG"
     gene            1764..2900
                     /locus_tag="VGP50_03145"
     CDS             1764..2900
                     /locus_tag="VGP50_03145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV8026397.1"
                     /translation="MSDEDVFDEAEAPGVAMAVPAPRENPVLLGHEAAEAALLGAYRS
                     GRLPHAWLLSGPRGIGKATLAFRFARFVLAGGGAEEGLFAAAAPAGLAVAPTHPVFRR
                     VASGGHADLLVVERGVDPKRKKLRSEIVVDDTRAIAGFLRLTPAEGHWRVVVVDGADL
                     MNRNAANALLKILEEPPQKALLLLMSDNPGRILPTIRSRCRMLPLKPLGGAAVEEALA
                     RYRPDLAPADRAALARLAEGSIGRALDLAAAGGLGLYRALLKLLEKLPQLDGVALHAL
                     ADRLARGDGEDAFRLASELLPGWVARMVTLAAGGNGAGAALPGEAEAMRRLAQRRSLE
                     QWIEVWENLTHLFTLADGVNLDRKQVVLNAFFALEEAARGAAQH"
     gene            2920..>3317
                     /locus_tag="VGP50_03150"
     CDS             2920..>3317
                     /locus_tag="VGP50_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV8026398.1"
                     /translation="MTGAEPFYVTTPIYYVNDALHLGHAYTTVACDALARFMRLDGRP
                     VHFLTGTDEHGQKVEKSAQAAGLDPQTFCDRASQKARDMTVLMNISNDDFIRTTEPRH
                     TRSCQALWRAIAARKTPKGRDNIYLGKYAG"
BASE COUNT          516 a         1142 c         1154 g          505 t
ORIGIN      
        1 cgcgcatgtt cgtgccctat ccggggcagg tgaagatcga ggatctgctg cgcggcgtca
       61 tcgtgcagtc cggcaacgac gcttgcgtcg tgctggccga gggcctggcc ggcagcgagg
      121 ccgccttcgt cgagcggatg aacgcgacgg cggagaagct cggcctcacc aacagccact
      181 tcgccaatgt gcatggcctg ccggcgcccg gccactacat gtcgccgcgc gacctcacca
      241 tgctgtcgcg ccacctcatc caggacttcc cgcagtatta tcgctacgag gccgagaagg
      301 agttcaccta taacggcgtc aagcaaggca accgcaaccc gctgctctat cgcaacatcg
      361 gcgccgacgg catcaagacc gggcataccg aggaagcggg ctacggcctc gtcggctcgg
      421 cgatccgcag cggccggcgc gtcatcgtca tgctctccgg catgagcagc atgaaggagc
      481 gcgacgtcga gagcgagaag gtgctcgaat gggcctatcg cgagttcaac gactacacca
      541 tcggcaaggc cggcgagccg atcgacgagg cgccggtctg gctcggcgcc catgccaaag
      601 tccccgccgc cccggcggag gatctcgtgg tcacgctgcc gcgcgcggcg cggcgcgacc
      661 tcaaggtcat ggcggtctat gacggcgcgg tgaaggcgcc gatcgccaag ggccagaccg
      721 tcggcaagct ggtggtgacg gcgcccgaca ccgacaccat cgagacgccg ctggtggcga
      781 cgcaagccgt cgaccgcctc gacgccctca gccgcgtcgg cgtcggcgtc gggtatctgc
      841 tgctggggca caagaactga gtctgcatcg tcgcggcacc ccccacgcta accctccccc
      901 tcaagggggg agggaagtcg aacgtgcggg aagcattctc cctcccccct tgaaggggag
      961 ggccggggtg gggggtacgg cgcggtgacg gcaaagcacg gcttcttctg cgttttcgag
     1021 ggtgtcgacg gcagcggcaa gaccggcacg ctcgcgcaca tcgagagcgc gctcgccaag
     1081 gacggtgtcg cgctcctcgc gacgcgcgag cccggcggca cggaggaggg gttggcgctg
     1141 cgcaaactgc tgctgggcgg cagccatcgc tggacgccca acgccgagct gctgctggtc
     1201 aatgccgcgc gcgcgcagca tgtggagaag gtgctccgtc cggcgctcgg cgacggcaag
     1261 ctcgtgctct gcgatcgctt catcggctcg acgctcgctt atcaaggcgc gggacacggc
     1321 atcggcgacg agcggatccg cgcgctccac aagatcgccg ccggcgatct ctggccggac
     1381 ctgacgatcc tgctcgatgt cgatcccgag cttgcggtga agcgcagccg ccggcgcctc
     1441 gcagcaagcg ccagcgacga aggccgcttc gaagcgctcg acctcgattt ccatcgccgc
     1501 gtccgccgat ccttcctcga ccaggcggcg cgcgcgccga cggcgcatgt cgtgctcgat
     1561 gcgagccggc cgcagcaaat cgtcgaggcg gacgcgctgg ccgcgctgcg cgcggcgctc
     1621 aaggcgcgcg gatagcttca cccctcaccc tcccgccgcc aagcggcggg cccctccctc
     1681 tcccgcaagg ggagagggga ttttcagcgc ttgcagcatg ccccgcggcg cggccggctt
     1741 gatcggcgcg ctataatggc gcgatgagcg atgaggatgt gttcgacgag gcggaagcgc
     1801 cgggggtggc gatggcggtt ccggcgccgc gcgagaatcc ggtgctgctg ggacatgagg
     1861 cggcggaagc ggctctgctc ggggcttatc gctcgggccg gctgccgcat gcctggctgc
     1921 tctcggggcc gcgcggcatc ggcaaggcga cgctcgcttt ccgcttcgcg cgcttcgtgc
     1981 tggccggcgg cggagcggag gaggggcttt tcgccgccgc ggcgcccgcc ggcctggcgg
     2041 tcgcgccgac gcatcccgtg ttccgccgcg tcgcctcggg cggccatgcg gatctcctgg
     2101 tggtcgagcg cggcgtcgat cccaagcgca agaagctgcg cagcgagatc gtcgtcgatg
     2161 acacgcgggc gatcgccggc tttcttcggc tgacccccgc ggaaggccat tggcgggtcg
     2221 tggttgtcga tggtgccgac ctgatgaacc gtaatgctgc caatgccttg ctcaagatcc
     2281 ttgaagagcc gcctcaaaag gcgctgctgc tgctgatgag cgacaacccc ggccgcatcc
     2341 tgcccaccat ccgctcgcgc tgccgcatgc tgccgttgaa accgctcggc ggagcggcgg
     2401 tggaggaggc gctcgcgcgc taccggccgg atctggcgcc ggccgaccgg gccgcgctgg
     2461 cgcgcttggc ggaaggcagc atcggccgcg ctcttgatct cgcagcagca ggcgggctgg
     2521 ggctttaccg ggcgcttttg aagctgcttg agaagttgcc gcagctcgac ggcgtggccc
     2581 tccatgcgtt ggccgaccgg ctggcgcggg gcgacggcga ggacgcgttc cgtctcgcgt
     2641 cggagctgct gcccggatgg gttgcccgca tggtgacgtt ggccgccggc ggcaacgggg
     2701 cgggcgcagc gctccccggc gaagccgagg cgatgcgccg ccttgcccag cgccgcagcc
     2761 ttgaacaatg gattgaggta tgggagaacc tcacccatct ttttaccctg gccgatggcg
     2821 tcaacctcga ccgcaagcag gtcgtgctga acgccttttt cgccctcgag gaagccgcgc
     2881 gcggcgcggc gcagcattga cccgccttcg ggagaaagta tgaccggcgc tgagcccttc
     2941 tatgtcacga cgccgatcta ttacgtgaac gatgcgttgc atctcggcca cgcctacacc
     3001 acggtggcct gcgatgcgct ggcgcgcttc atgcggctcg acggccggcc cgtccatttc
     3061 ctcaccggca ccgacgagca tggccagaag gtcgagaagt cggcgcaggc cgcgggactc
     3121 gatccccaga ccttctgcga tcgcgcgtcg cagaaggccc gcgacatgac ggtgctgatg
     3181 aacatcagca acgacgactt catccgcacc accgagccgc ggcacacgcg ctcgtgccag
     3241 gcgctctggc gcgccatcgc ggcgcgcaaa acgcccaagg gccgcgacaa catctatctc
     3301 ggcaaatatg ccggctg
//
LOCUS       DASYNS010000100         8327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2520828, whole genome shotgun sequence.
ACCESSION   DASYNS010000100 DASYNS010000000
VERSION     DASYNS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2520828"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..901)
                     /locus_tag="VGP50_03155"
     CDS             complement(245..901)
                     /locus_tag="VGP50_03155"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEV8026399.1"
                     /translation="MYLPKDFHEERVPLLHDAIRRIALGTLVTLGADGMEASHVPMLI
                     DPEPQPFGTLRGHLARANPQWHRIAAGTEALVIFLGPETYISPSWYATKRATGKVVPT
                     WNYIAIHAYGALRIVDDAEWLRALVTRLTETHEGSRAEPWAVSDAPADYIAGMLKAIV
                     GFELPIIRLEGKYKMSQNRPAEDRAGVFEGLKREGHPGMAWIVRSAAWLVKSAAPKKP
                     "
     gene            complement(903..1679)
                     /locus_tag="VGP50_03160"
     CDS             complement(903..1679)
                     /locus_tag="VGP50_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEV8026400.1"
                     /translation="MRMDMPQNRFKRALAQGTRQIGLWMSLASPTATEVAAGAGFDWL
                     LLDMEHAPNELPDIAHHLRAAEGGTAEPVVRVPWNDPVIVKRLLDIGAKSLLFPFVQS
                     AEEARRAVAATRYPPGGIRGFAGTTRANRYGRVKDYAKRAAEEICVLVQCETRKAVAD
                     IPAIAAVEGIDGIFIGPADLAADMGHLADTQHADVQAAILAAGKAIRAHGKAPGFLSV
                     REDETRRVLEAGFVFVAVGTDVTLLARHAEALLRNFKSGD"
     gene            complement(1679..3412)
                     /gene="araD"
                     /locus_tag="VGP50_03165"
     CDS             complement(1679..3412)
                     /gene="araD"
                     /locus_tag="VGP50_03165"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEV8026401.1"
                     /translation="MTAPRRKTYAELRSARWYNAADLRSFGHRSRTKQMGFAAEDYAG
                     KPVIAILNTWSEMNPCHQHFRTRAADVKRGVWQAGGFPVEMPAMALGEPFMKPSTMMY
                     RNLLAMEAEELLRANPIDGVVLMGGCDKTVPALVMGATSMNLPAIFVPAGPMLRGHWQ
                     GKELGSGSDVWKYWAELRAGNIDEEDWRGIEDGIARSAGTCMTMGTASTMAALVEALG
                     LSLTGASSIPAVDSSHARMAAASGRRIVDLVWQDVRPRDLLTEAAFDNAITTLMAIGG
                     STNAIIHLVAMAGRAGIALPLERFDALARRTPLLANIRPSGKYLMEDFFYAGGLPALL
                     RQLGTLLHGSAMTASGRTLADDIAGAEIYNDDVIRSSNRALQPEGGLAVLKGNLAPNG
                     AVIKPSAMEARLQRHRGPAVVFRDYNDMAQRIDDPALEVTADSVLVLQNGGPQGGPGM
                     PEWGQLPIPKKLLRDGVRDMLRISDARMSGTSYGACVLHVSPESYVGGPLALVRSGDV
                     IAIDVPGRSLELAVPDAELQRRRAAWTPPTPRFARSYGALYHRHIGQAETGCDFDFLA
                     GTAAVPEPEIH"
     gene            3767..4831
                     /locus_tag="VGP50_03170"
     CDS             3767..4831
                     /locus_tag="VGP50_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV8026402.1"
                     /translation="MKEQRRIPVAMAVLAAAAFVEASAASAQELPGPYAGVGVGANFL
                     QNMTVSDGLGSTTLKYDPGPIATGELGYALGNGFRGEFQYGYRHSDANSTSLPTGPAT
                     AFGLKGNAAVNSYMVNGLYDFRVAPSLWANVGAGLGAATVSLNDLGNHTAFAYQAMTG
                     IEYGLTPRLKIGVEYDFLGTNPLSLQSTSPVTSHPTYLDHAVLLTLRYSFGPAPVRAA
                     AVVTPPAPPVTAPAGTTAPPPPFSRDFSIYFATGSDKLTPDARETVRQAAEATTANAP
                     THINVGGHTDTVGSASYNERLSARRAESVRRELIADGVASDQITTRAFGESDLAVPTA
                     DNVPEKANRRVVIVVQGPGT"
     gene            4933..6120
                     /locus_tag="VGP50_03175"
     CDS             4933..6120
                     /locus_tag="VGP50_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026403.1"
                     /translation="MQRSRDRILTTHTGSLPRPKELTQLLVRRARGEAVDAAALERAA
                     AAALRWVVPQQMAAGIDVGNDGEQQRESFFLHLVHRMSGFGGSWKRWPRADVEHYPAF
                     KQALEQQAAAKEAVGSFSPPKAIGAVRYVDASLVHRECADFRAVLGETGSRFVDAFLT
                     APSPGIVAAAIRNEHYDSDDAYLAALGEALRVEYEAIAGHGFVLQLDCPDLALERHVS
                     YQDRPLGEFLGFCERVVETINRALVNIPCERVRMHVCWGNYEAPHDCDVPLPEILPIL
                     KQAKVGALSLPFANPRHAHEVRYLEKWLGDDQFVIVGVIDTLTNFVEHPEVVAGRSER
                     VARIVGDPRRVMAGTDCGFDTSAGMGRVAEDVVWAKLAALGEGARLASQRLFAHPDDI
                     GVS"
     gene            6219..6710
                     /locus_tag="VGP50_03180"
     CDS             6219..6710
                     /locus_tag="VGP50_03180"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HEV8026404.1"
                     /translation="MRRLSTVAALLIGIGGTALAEDATTPPKGSTSLMEVLGEGVQVY
                     ACAATDHDYAWVFRSPEAVLLDAQGHQVGSHGAGPTWTLADGSSVIGEKVAEAASPDP
                     HAIPWLLLRVKSHDGTGQLADAAWVRRANTVGGKAPETGCDPTHVGETARIRYSAVYQ
                     FLR"
     gene            complement(6711..8189)
                     /locus_tag="VGP50_03185"
     CDS             complement(6711..8189)
                     /locus_tag="VGP50_03185"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV8026405.1"
                     /translation="MIAPFRRPLRVKLILPALTEATSPYWRPIKYSLFPPLGLATIAA
                     YLDPEDEAEIVDEHVMPLDTDDAPDLVLVQVYITNAYRAYGIADGYRARGVFVALGGL
                     HVTSLPEEAASHADTIFLGPGEQSFPHFLADFRAGAPRPLYASTAGRTLERVPPLRRD
                     LIRRSHYLVPNSLVVTRGCPQHCDFCYKDAFFAGGRGFYTQRVDDALAEIERLPGRHL
                     YFLDDHLLGDRRFAAALFEGLRGMNRLFQGAATVDSILRGDLIERAAAAGLRSIFVGF
                     ETLSPANLVASNKRQNLGRDYKAVTDRLHGLGIMINGSFVFGMDDDGEDVFRRTVDWA
                     IAHGITTATFHIQTPYPGTRLFARMEEEGRIVTRDWDRYDTRHVVYRPARLAPEALKT
                     GYDWAYREFYTWSAIARASLAHGTLKHQAKHFFYAAGWKKFEPLWDLMIRARQLRRVT
                     PVLEAVLAKVTRKPALLSRDRDATNPLSRKGRGDIVARLKQF"
     gene            complement(8186..>8327)
                     /locus_tag="VGP50_03190"
     CDS             complement(8186..>8327)
                     /locus_tag="VGP50_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HEV8026406.1"
                     /translation="AELWFSDTMWPDFDAASLGQALADFRRRERRFGGLPASALAQGA
                     AE"
BASE COUNT         1335 a         2828 c         2850 g         1314 t
ORIGIN      
        1 ccggccgtca tcgacgccat cgcccggcgc cgtctcgtgc cggtgagcgt cggcgcgcgc
       61 atcttcggcg cgccccgtcg cggcagcagc gacgcgcctc gccactgcca cctcatcgcg
      121 tgagaatcgg cgtgcgatac ccgtcgcgca cgccacatag agacattcgc atgaatgccc
      181 ctctcccgca tcgcgagagg ggcaagtttg gcgctcgcgg aggcagcggt gtcggtggac
      241 tagatcacgg cttcttcggc gccgcgctct tgacgagcca ggccgcgctc ctgacgatcc
      301 aagccatgcc ggggtgtcct tcgcgcttca gtccttcgaa gacgccggcg cggtcctcgg
      361 ccgggcggtt ctgactcatt ttatatttgc cctcgagccg gatgatcggc agctcgaagc
      421 cgacgatcgc tttgagcatg ccggcgatgt agtcggccgg cgcgtcgctg accgcccagg
      481 gctcggcgcg gctaccctcg tgagtctccg tcagccgcgt caccagcgcc cgcagccatt
      541 cggcatcgtc gacgatgcgg agcgcgccat aagcgtggat ggcgatatag ttccaggtgg
      601 gcacgacctt gccggtcgcg cgcttcgtcg cataccagga gggcgagata taggtctcgg
      661 gcccgagaaa gatgacgagc gcctcagtgc ccgcggcaat gcggtgccat tgcgggttgg
      721 cgcgggcgag atggccgcgc agcgtgccga agggctgcgg ctcgggatcg atgagcatcg
      781 gcacatggct tgcctccatc ccgtccgcgc ccagcgtcac cagcgttccg agcgcgatcc
      841 ggcggatggc gtcatgcagc aggggaacgc gctcttcgtg gaaatccttc ggcagataca
      901 tgtcagtcgc cgctcttgaa attgcgcagc agcgcctcgg catggcgcgc cagcagcgtc
      961 acgtcggtgc cgacggcgac gaagacgaag cccgcctcga gcacgcgccg cgtctcgtcc
     1021 tcgcggacgc tcaggaatcc tggcgccttg ccatgggcgc ggatcgcctt gcccgccgcg
     1081 aggatcgctg cctgcacgtc ggcgtgctgc gtgtcggcga gatggcccat atcggcggcg
     1141 agatcggcag gaccgatgaa gatgccgtcg atgccctcca ccgcggcgat cgccggaatg
     1201 tccgccaccg ccttgcgcgt ctcgcattgc acgaggacgc agatctcctc ggctgcgcgc
     1261 ttcgcgtaat ccttgacgcg gccatagcgg ttggcgcggg tggtgccggc gaagccgcgg
     1321 attccaccgg gcggatagcg cgtcgccgcc acggcgcgcc gcgcctcctc ggccgattgc
     1381 acgaagggga agagcaggct cttcgctccg atgtcgagga ggcgcttgac gatgaccggg
     1441 tcgttccacg gcacgcgcac gacgggctcg gccgtgccgc cctcggcggc gcgcagatga
     1501 tgcgcgatgt cgggaagctc gttgggcgca tgctccatgt cgagcaacag ccaatcgaag
     1561 ccggcgccgg ccgcgacttc ggtcgctgtc gggctcgcga ggctcatcca caacccgatc
     1621 tgccgcgtgc cctgggcgag cgcgcgcttg aaacggttct gcggcatgtc catgcgcatc
     1681 aatgaatctc cggctccggc acggccgccg tcccggcgag gaaatcgaaa tcgcagccgg
     1741 tttcggcctg gccgatatgg cgatgataga gcgcgccata gctgcgcgcg aagcgcggcg
     1801 tgggcggcgt ccaggcggcg cgccggcgct ggagctccgc gtccgggacc gcgagttcga
     1861 ggctgcgccc cggcacgtcg atggcgatga cgtcgccgct ccgcaccagc gccagcggcc
     1921 cgccgacata ggattcgggc gagacatgga gcacgcaggc gccgtagctg gtgccgctca
     1981 tccgcgcatc ggagatgcgc agcatgtcgc gcacgccgtc cctgagcagc ttcttcggga
     2041 tcggcagctg gccccattcg ggcatgcccg gcccgccctg cgggccgcca ttctgcagca
     2101 ccagcacgga gtcggccgtg acctcgagcg ccggatcgtc gatgcgttgc gccatgtcgt
     2161 tgtaatcgcg gaataccacc gccggccccc gatgccgctg cagccgcgcc tccatggcgg
     2221 acggcttgat gacggcgccg ttgggcgcga gattgccttt gagcacggca agcccgccct
     2281 cgggctggag cgcgcgattg gacgagcgga tgacgtcgtc gttgtagatc tcagcgcccg
     2341 cgatgtcgtc ggcgagcgtg cgcccgctcg ccgtcatggc gctgccgtgg agcagcgtgc
     2401 cgagctggcg cagcagcgcc ggcagcccgc ccgcgtagaa gaaatcctcc atcaggtatt
     2461 tccccgacgg ccggatattg gcgagaagcg gtgtccgccg cgcgagcgcg tcaaagcgct
     2521 cgagcggcag cgcgatgccg gcgcggcccg ccatggcgac gagatgaatg atggcgttgg
     2581 tcgagccgcc gatcgccatc agcgtcgtga tggcgttgtc gaaggccgcc tcggtcagga
     2641 gatcgcgggg acgcacatcc tgccacacga gatcgacgat gcgccgcccg ctcgcggccg
     2701 ccatgcgcgc atgcgatgaa tcaaccgccg ggatcgacga cgcgccggtc agcgacagtc
     2761 cgagcgcttc caccagcgcc gccatcgtcg acgccgtgcc catggtcatg caggtcccgg
     2821 ccgagcgcgc gatgccgtcc tcgatgccgc gccaatcttc ttcgtcgatg tttccggcac
     2881 ggagctccgc ccagtatttc cagacatcgc tgcccgagcc cagctccttc ccctgccaat
     2941 ggccgcgcag catcggcccc gccggcacga agatcgccgg caggttcatg ctggtggcgc
     3001 ccatgacgag ggccggcacg gtcttgtcgc agccgcccat cagcacgacg ccgtcgatcg
     3061 ggttggcgcg cagcagctcc tccgcctcca tggcgaggag gttgcggtac atcatcgtcg
     3121 agggcttcat gaagggctcg cccagcgcca tcgccggcat ctcgacggga aagccgccgg
     3181 cctgccacac gccccgcttc acgtccgccg cgcgcgtgcg gaagtgctgg tggcacgggt
     3241 tcatctcgct ccaggtgttg aggatggcga tgaccggctt gccggcatag tcctcggcgg
     3301 cgaagcccat ctgcttggtg cgcgagcgat ggccgaaaga gcgaagatcc gcggcgttgt
     3361 accagcgcgc gctgcgcaac tcggcgtagg tcttgcgacg cggcgcggtc acgggcacgc
     3421 ctcctttcgg gcaagccgag tgaagcgatg gcggccgcgc ctgtcaatcg cgaggcgcgc
     3481 ggctacggtt atcggcggcg ccgtgcccca ccggccacaa cgtctgcgaa atcctgcgcg
     3541 gcccgggaac atgcgggctc aatcattgtt caaggcggca gcaacgccgg cacgcgccgg
     3601 tattgccgtt ggggaaatcc agtgactcgg gcgcccgtcc gagtgggtga atatgcccga
     3661 cctgcagcgt cttgatgcgc gtcgctccgc gacgcgtccg gtatctgcct caggcggatt
     3721 cccggagagt tgatcgaggt cgagttttca gcggggaggt tggatcatga aagagcagcg
     3781 ccggattccg gttgcgatgg ctgtgctggc tgcggccgcg ttcgtcgagg ccagcgcggc
     3841 ctcggcacag gagttgcctg gcccttatgc cggcgtgggc gtcggcgcga atttcctgca
     3901 aaacatgacg gtgagcgacg ggctcggctc gacgacactg aaatacgacc cgggccccat
     3961 cgccacgggc gagctcggct acgcgctcgg caatggtttc cgcggtgaat tccaatacgg
     4021 ctatcgccac agcgatgcca acagcacctc gctccccacc ggcccggcga cggctttcgg
     4081 cctcaagggg aacgcggccg tcaatagcta tatggtcaat gggctttacg actttcgcgt
     4141 cgcgccgtca ttgtgggcga atgtcggcgc cggcctgggc gcggccacgg tcagcctgaa
     4201 cgacctcggc aaccatacgg cattcgccta tcaagcgatg accggcatcg aatacgggct
     4261 cacgccgcgg ctgaagatcg gcgtcgaata cgatttcctc ggcaccaacc cgctcagcct
     4321 gcagtcgacg tcgcccgtca cctcgcatcc gacctacctc gaccatgcgg tcctgctgac
     4381 gctgcgctac agcttcgggc cggcgccggt gcgggcagcc gccgtcgtga cgccgccggc
     4441 gccgccggtc acggcgccgg ccggaaccac cgcgccgccg ccgcccttct cgcgcgattt
     4501 ctccatctat ttcgcgaccg gcagcgacaa gctcaccccg gacgcccgcg aaaccgttcg
     4561 ccaggccgca gaggccacga cggccaatgc gccgacgcac atcaatgtcg gcggccacac
     4621 cgacacggtc ggctcggcga gctataacga acggctttct gcccggcgcg ccgaatcggt
     4681 ccgcagggaa ctgatcgccg acggcgtcgc ctccgaccag atcacgaccc gcgcctttgg
     4741 cgaaagcgac ctcgcggtgc cgaccgccga caacgtaccg gagaaggcca accgccgcgt
     4801 cgtcatcgtc gtgcaaggac ccggtacgta gaccatgccg agcgccttgc cgtgccaatg
     4861 gccccgccgg aacgcggaat tgctggcggt ggacagttgg actccaatct ggcgcgtggc
     4921 aagatcgccg ccatgcaacg gagccgggac cgcatcctga ccacccacac cggcagcctg
     4981 ccgcggccga aggagctgac gcagctgctg gtgcggcgcg cgcgcggcga ggcggtcgat
     5041 gcggcagcgc tggagcgggc cgcggcggcg gcgctgcgct gggtggtgcc gcagcagatg
     5101 gccgccggca tcgatgtcgg gaatgatggc gagcagcagc gcgaaagctt cttcctgcat
     5161 ctcgtccacc gcatgagcgg cttcggcggc agctggaagc gttggccgcg cgccgatgtc
     5221 gagcactacc ccgccttcaa gcaggcgctc gagcagcagg cggcggcgaa ggaggcggtc
     5281 ggcagcttca gcccgcccaa ggcgatcggc gcggtgcgct atgtcgacgc ttccctcgtc
     5341 catcgcgaat gcgcggattt tcgcgccgtg ctcggcgaga ccggatcgcg cttcgtcgac
     5401 gcgttcctca cagcgccctc gccggggatc gtcgcggcgg cgatccgcaa cgagcattac
     5461 gacagcgacg acgcctatct cgccgcgctg ggcgaggcgc tccgcgtcga atacgaggcg
     5521 atcgcgggcc atggcttcgt gctccagctc gactgccccg acctcgcgct cgagcggcat
     5581 gtttcctatc aggaccgtcc gctcggcgag ttcctcggct tctgcgagcg cgtggtcgag
     5641 accatcaacc gggcgctggt gaacatcccg tgcgagcgcg tccggatgca tgtgtgctgg
     5701 ggcaattacg aggcgccgca tgattgcgac gtgccgctgc cggaaatcct gccgatcctg
     5761 aaacaggcga aggtcggcgc gctgtcgctg cccttcgcca atccgcgcca cgcgcatgaa
     5821 gtgcgctatc tggaaaaatg gctcggcgac gaccagttcg tcatcgtcgg cgtcatcgac
     5881 accttgacca atttcgtcga gcatcccgag gtcgtggccg ggcggagcga gcgcgtcgcg
     5941 cgcatcgtcg gcgatccccg ccgcgtgatg gccggcaccg attgcggctt cgacacctcg
     6001 gccggcatgg gccgcgtcgc agaggacgtc gtctgggcga agctcgcggc actcggcgag
     6061 ggtgcgcggc tggcgtcgca acggctgttc gcacatcccg acgacattgg cgtgagctga
     6121 tccgatcaag atctcgccgc tcgggcggcg tcgtttcgct ccccgacgca atcaactagg
     6181 atccgtcgca agagcgcgtt cggttgcgag gaaaaggcat gagacgtttg agtacggtcg
     6241 cggcgctgct gatcggcatt ggcggcacgg cgctggccga agacgcaacc acaccgccga
     6301 agggttccac cagcctgatg gaagtcttgg gtgaaggcgt tcaggtctac gcctgcgccg
     6361 ccaccgacca cgactacgcc tgggtctttc gcagcccgga ggccgtcctc ctcgatgcgc
     6421 aggggcatca ggtcggcagc catggcgcgg gaccgacctg gacgctcgcc gacggctcct
     6481 cagtcatcgg cgagaaggtc gcggaagcag catcgcccga tccgcatgcg atcccctggc
     6541 tcctcttgcg cgtaaagtcc catgacggaa ccgggcagtt ggccgatgcg gcctgggtcc
     6601 gccgcgcgaa taccgtcggc ggcaaagccc ccgagacggg ctgcgatccg acccatgtgg
     6661 gagaaacggc gcgcatccgt tattcggccg tctaccagtt cctgcgctaa tcagaactgc
     6721 ttgagtcgag ccacgatatc ccctctcccc ttgcgggaga ggggatttgt agcatccctg
     6781 tcgcgcgaca gaagtgccgg cttccgcgtg accttggcca gcaccgcctc gagcacgggc
     6841 gtcacccgtc ggagctggcg cgcgcgaatc atcagatccc agagcggctc gaacttcttc
     6901 cagccggccg cgtagaagaa gtgcttggcc tgatgcttca gcgtgccatg ggcgagcgac
     6961 gcgcgggcga tcgcggacca ggtgtagaat tcgcgatagg cccagtcata gccggttttg
     7021 agcgcctcgg gcgcgagccg cgccgggcga tagaccacat gccgcgtgtc gtagcgatcc
     7081 cagtcgcgcg tgacgatgcg gccctcctct tccatccgcg cgaagaggcg cgtgccggga
     7141 tagggcgtct ggatgtgaaa cgtcgccgtc gtgatgccat gggcgatcgc ccagtcgacg
     7201 gtgcggcgga agacgtcctc gccgtcatcg tccatgccga agacgaagct gccattgatc
     7261 atgatgccga ggccatgcag ccggtcggtg acggccttgt agtcgcgccc cagattctgc
     7321 cgcttgttgc tcgcgaccag attggccggc gacaaggtct cgaagccgac gaagatgctg
     7381 cgcagtcccg cggccgccgc gcgctcgatg aggtcgccgc gcaggatgct gtccaccgtc
     7441 gcggcgccct ggaagaggcg gttcatgccg cgcaacccct cgaagagagc cgctgcgaaa
     7501 cggcggtcgc ccagcaaatg atcgtcgaga aaatagagat ggcgccccgg cagccgctcg
     7561 atctcggcca gcgcgtcgtc gacgcgctgc gtgtaaaagc cgcggccgcc ggcgaagaag
     7621 gcgtccttgt agcagaagtc gcaatgctgc gggcagccgc gggtcaccac cagcgagttc
     7681 ggcacgagat agtggctgcg gcggatgaga tcgcgccgca gcggcggcac gcgctcgagc
     7741 gtgcgtcccg cggtcgaggc atagagcggg cgcggcgcgc cggcgcggaa atcggcgagg
     7801 aagtgcggga agctctgctc gcccgggccg aggaagatgg tatcggcatg cgaagcggct
     7861 tcttccggca acgaggtcac atggagcccg cccagcgcca cgaagacgcc tctcgcgcga
     7921 tagccgtcgg cgatgccata ggcgcggtag gcattggtga tatagacctg gaccagcacg
     7981 agatcgggcg cgtcgtccgt atcgagcggc atgacatgct cgtcgacgat ctcggcctcg
     8041 tcctcggggt cgagataggc cgcgatggtg gcgagcccga gcggcgggaa gagcgaatat
     8101 ttgatgggcc gccagtaggg gctcgtcgcc tccgtcaagg ccggcaggat gagcttgacg
     8161 cggagcggcc ggcggaacgg cgcgatcatt cggccgctcc ctgggcgagc gcgctcgccg
     8221 gaagcccgcc gaagcgtcgc tcgcgacggc ggaaatcggc aagcgcctga ccgagcgacg
     8281 ccgcgtcgaa atcgggccac atggtgtcgc tgaaccagag ctcggca
//
LOCUS       DASYNS010000101         9157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2052400, whole genome shotgun sequence.
ACCESSION   DASYNS010000101 DASYNS010000000
VERSION     DASYNS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 9157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9157
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2052400"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VGP50_03195"
     CDS             complement(<1..192)
                     /locus_tag="VGP50_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8026407.1"
                     /translation="MVSLDLLLSAIVAGILLGGFYAAMTVGVSISFGMLDIVNIAHPA
                     FIILGSYIAYIVNARLGIDP"
     gene            584..1345
                     /locus_tag="VGP50_03200"
     CDS             584..1345
                     /locus_tag="VGP50_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEV8026408.1"
                     /translation="MIEQILPANVFAAMLIFSRVGSAMMLLPGFGELYVMQRYRLLLA
                     LLIALLLTPVLGPSLPPRPVDVASLLTLVGGEVVIGIFLGTVARVLLSALDTAGTVIS
                     FQLGLSAAQIFNPMAATQGSVTASFYSVLGVLLMFITDLHHLLIGAIVDSYDVFVPGT
                     LPAVGDLSESVVRSASAAFRLGAEIAAPFILLGTLFFVAIGIISRLVPQLQLLFVTQP
                     LQIVGGFVVLGLVLSAGMEWFLASFAQQFVTLVGG"
     gene            1352..2431
                     /gene="flhB"
                     /locus_tag="VGP50_03205"
     CDS             1352..2431
                     /gene="flhB"
                     /locus_tag="VGP50_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HEV8026409.1"
                     /translation="MSDTDSDQRTEQPSQRKLAQARGQGQVAQSREINSWFVLATGAA
                     VVLLWGPSLARPLQATLARFLDPASLLDGDGILWDAVRSMLGTVATSFALPLALFVAA
                     ALAGSLVQTGLVLATDRLGVDPSRVSPIQGFSRLFSLRSASEFIKSLIKVAIIAAAIV
                     RLLAPELDRLSLLTKVGVPGLIGEIYQGLLRVALSVLVALAAIALLDYFYQRFAFMRG
                     QRMSKQEVKEEHKQTEGDPRVKARLRQIRMERARKRMMAAVPGASVVVTNPTHYAVAL
                     KYEMGEMGAPKVVAKGADLIADRIREIARENDVPIVENPPLARALYGGVEIDHEIPPE
                     HYRAVAEVINYVFRLKGKIKARTGS"
     gene            2500..5058
                     /locus_tag="VGP50_03210"
     CDS             2500..5058
                     /locus_tag="VGP50_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8026410.1"
                     /translation="MTMQPAMSGGALPSATSPADMGMPAWTRWLGTIALALAALALLL
                     FAVSLAEFVEGATARPVAVMLIVFAVICGVAALVARKVHGALAGRLDLLSQALEASPN
                     AHLIVAPDGLIAYANAAFHRFFPELAEPPLQAIERRFGEDERSAVEFARLRSEVVRGG
                     ATAQLALRNGAGPATWFSLSAHLLAGRPGYGLWSFEDVTTRHEMEQVIRDEQMKLADF
                     LDHAPTGFYSVDGEGRFLFVNQALADWLGASPTALVENGAKLRDFIISDVPPGTPPHA
                     PFEGGAAGAMRGEVVLRGRQGRTVYASISQSAVQVGDGLRTRSVVRDLTPEREWEEAL
                     RQSRRRFQRFFANAPVGIALLDRTGRLQEANRALGELFGAPPAELIGKTLVSFLTPES
                     QGEVAAKLASVAEGRLASRPVEIRLAGPRDKSAALFVSRVGAAEEGEAAVILHFIDTT
                     EQKSLEVQFAQSQKMQAVGQLAGGVAHDFNNLLTAMIGFCDLLLLRFRPGDPSFADIM
                     QIKQNANRAANLVRQLLAFSRQQTLQPRILNITDVLAELSHLLRRLIGENIELKVVHG
                     RDLGLVKVDQGQLEQVIINLAVNARDAMPGGGTLTIRTGNVTSEFPMRRGHEVMPPGE
                     YVQVEVTDTGIGIPKENLVRIFEPFFSTKEVGSGTGLGLSTVYGIVKQTGGFVFVDSE
                     PGKGAAFSIYLPRHAGAAAGAGAEAGAAARSGADLADAQATRDLTGTGTVLLVEDEDP
                     VRLFSARALRNKGYKVIEAKSGEAALEVITGGGEKVELLITDVVMPRMDGPSLVKRVR
                     ELYPDMKVIFISGYTEDAFRKRLDRDGDIHFLPKPFSLKQLATKVKEVMLAAPG"
     gene            complement(5115..5567)
                     /locus_tag="VGP50_03215"
     CDS             complement(5115..5567)
                     /locus_tag="VGP50_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEV8026411.1"
                     /translation="MKKSDASQGPSASELISKRIAELGDWRGETLGRIRKLIKEADPE
                     IVEEWKWMGTPVWSHDGIICTGESYKNVVKLTFAKGASLRDPARLFNSSLDGNTRRAI
                     DIHEGEEVDKSAFKALLRQAIALNTSGKSKPSKGAKPSKRAKPSKRAD"
     gene            complement(5621..6031)
                     /locus_tag="VGP50_03220"
     CDS             complement(5621..6031)
                     /locus_tag="VGP50_03220"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV8026412.1"
                     /translation="MSKPATETRSVVVEREIPHPPEKIWRALTQPHLIEEWLMKNDFK
                     PVVSHRFNLRADWGAVDCQVLAIEPNKTLSYTWAAYGLESVVTWTLTPTGAGTRLRME
                     QSSFRPDQQQAYHGAKAGWQRFFAALEQALARID"
     gene            complement(6028..6351)
                     /locus_tag="VGP50_03225"
     CDS             complement(6028..6351)
                     /locus_tag="VGP50_03225"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV8026413.1"
                     /translation="MPNAHDVLFRTLADPTRRAIFERLCRKGEQTVGALTARAGVSQP
                     AVSKHLGILKQAGLVRDRHEGRQTHYSAQLGALAPLIDWTSRMAGFWQSRFDDLEDLL
                     KRMDQ"
     gene            6733..8022
                     /locus_tag="VGP50_03230"
     CDS             6733..8022
                     /locus_tag="VGP50_03230"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV8026414.1"
                     /translation="MVGGGAGGLKLATRLGDTLGRSGRARIALVEKRRAHLWKPLLHA
                     VAAGSLNVDEHALDYLAQAHWHHFRYRLGEMVGVDRASKLVHVAHTPDDEGGEITPER
                     CFRYDTLVIATGSVSNDFGTPGASRHAIPLDTPEQAVRFHRRLVNACIRAHTQRGPVR
                     PGQLHVAIIGAGATGTELAAELHRTTRQVVGYGLDRIDPEKDVRITLIEAADRILPVL
                     PRRVSEATARLLAHLAVEVRTNARVIEVREDGVVLADGSLVPAELVVWAAGVKGPDVL
                     NRLDGLETNRANQLVVLPTLQTTRDPDVFAIGDCAGCPRPGHATPVPPRAQAAHQQAL
                     HLVKQLARRVDGKSTHAYVYRDFGALVSLGRYSTVGSLMGFLIGKSLFVEGWYARLIY
                     RSLYTRHETALHGLGTVLLGIMARTIIRRPQPVVKLH"
     gene            complement(8073..>9157)
                     /locus_tag="VGP50_03235"
     CDS             complement(8073..>9157)
                     /locus_tag="VGP50_03235"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEV8026415.1"
                     /translation="ALVMTGAPIGDNIFYRLCPTTLLLPLILLATLATIIASQSIITG
                     AFSMTRQAIQLGWCPRLRITQTSSEGFGQIYVATVNWLLMIVTVGLTIGFGNSDRLAA
                     AYGIAVSLTMLLTTVLLLVAMREVWHWNWAPSLAVASLFFCIDATFFAANATKVVEGG
                     WVPLTLAVLVYVVLTTWHRGTQAVAAGLVTMTEPADQFIARITAAGIARVPGTAVFLT
                     RTTRDAPPVMLWHVRKNRALHRHLLALKVVTASVPWVDAATRVTVERIAPDFFRATAR
                     YGFMERPDVPALLRQAKQLGCAVDLSDVTFYVGHETVLRRSDHKGLPGWQEHLFAFLQ
                     RNAAQLSDFLNLPSDATVEIGRQFEI"
BASE COUNT         1446 a         3047 c         3075 g         1589 t
ORIGIN      
        1 ggggtcgatg ccgaggcgcg cgttgacgat gtaggcgatg taggagccga ggatgatgaa
       61 agcggggtgc gcgatgttga cgatgtcgag catgccgaag gagatcgaca cgccgaccgt
      121 catggcggcg tagaagccgc cgaggagaat cccggcgacg atcgcgctca gcagcagatc
      181 gagggaaacc atgcgcgcag ctttacgctg ctgcgggcgt gctcgccccg tccagccgac
      241 gctcccacat tcctttaccc cccgcagttg cccggcctct ttgatcgagt cggtcgatta
      301 tgtcgcgacg tcagtctggc ggaagagcgc cgccttggcg agtcccgctg ggccatcgca
      361 tatgggtttc accacggagg cacggagaac acggagagga gaggttcggc gcgcgaagcg
      421 cgccattctt cgcttctccg tgtcctccgt gcctccgtgg tccatctttc ctactgcctt
      481 ctcggcagca tatgcgattg ccctgcgctc aaggggggag gggattcgct tgcacggcgg
      541 cacgaccttc tgcgatcgcg cggcgaagcc ccacgagtag gccatgatcg agcagatcct
      601 gccggcgaat gtgtttgcgg ccatgctcat cttctcgcgg gtgggttcgg cgatgatgct
      661 gctgccgggc ttcggcgaac tctatgtcat gcagcgctat cgcctgctgc tcgcgctgct
      721 gatcgcgctc ctgctgacgc cggtgctcgg gccgtcgctg ccgccgcggc cggtggacgt
      781 cgcgtcgctg ctcacgctgg tcggcggcga ggtggtcatc ggcatcttcc tcggcacggt
      841 cgcgcgcgtc ctgctctcgg cgctcgacac cgcgggcacc gtgatctcgt ttcagctcgg
      901 gctctcggcg gcgcagatct tcaacccgat ggcggcaacg caggggtcgg tcacggcgag
      961 cttctacagc gtgctgggcg tgctcttgat gttcatcacc gatctgcatc atttgctgat
     1021 cggcgccatc gtcgacagct acgacgtctt cgtgccgggc acgctgcccg cggtgggcga
     1081 tctctccgag tcggtggtgc gcagcgcctc cgccgcgttc cgcctcggcg ccgagatcgc
     1141 cgcacccttc atcctgctgg gcacgctctt cttcgtggcg atcggcatca tcagccggct
     1201 cgtgccgcag ctccagctcc tcttcgtcac gcagccgctg cagatcgtcg gcggcttcgt
     1261 cgtgctgggc ctggtgctga gcgccggcat ggagtggttc cttgcgagct tcgcccagca
     1321 attcgtcacg ctcgtgggcg gctgacgcgt catgtccgac accgacagcg accagcgcac
     1381 agagcaaccg tcgcaacgca agctggccca ggcgcgcggc cagggccagg tcgcgcaatc
     1441 gcgcgagatc aacagctggt tcgtgctggc cacgggtgcg gccgtcgtgc tgctgtgggg
     1501 gccgtcgctg gcgcggccgc tccaggccac gctcgcgcgc ttcctcgatc ccgccagcct
     1561 tctcgacggc gacggcatcc tctgggatgc cgtgcgcagc atgctcggga ccgtggcgac
     1621 gtccttcgcg ctgccgctcg cgctcttcgt cgccgccgcg ctggcgggct cgctggtgca
     1681 gacgggactg gtgctggcga ccgaccggct cggcgtcgat ccctcgcgcg tctcgccgat
     1741 ccagggcttc tcccgcctct tctcgctgcg ctcggcgagc gagttcatca agagcctcat
     1801 caaggtggcg atcatcgcgg ccgccattgt ccggctgctc gcgccggagc tcgaccgcct
     1861 gtcgctgttg acgaaggtcg gcgtgccggg gctcatcggg gagatctatc aggggctgct
     1921 gcgggtggcg ctgtcggtgc tggtggcgct cgcggcgatc gcgctcctcg actacttcta
     1981 ccagcgcttc gccttcatgc gcggccagcg catgtcgaaa caggaggtca aggaggagca
     2041 caagcagacc gagggcgacc ccagggtcaa ggcgcggctc cgccagatcc gcatggagcg
     2101 ggcgcgcaag cgcatgatgg ccgccgtgcc gggcgcgtcg gtggtggtga cgaacccgac
     2161 ccattatgcc gtcgcgctca agtacgagat gggcgagatg ggcgccccca aggtcgtcgc
     2221 caaaggcgcc gacctcatcg ccgaccggat ccgcgagatc gcgcgcgaga acgacgtgcc
     2281 gatcgtcgag aacccgccgc tggcgcgcgc gctctatggc ggcgtcgaga tcgaccatga
     2341 gatcccgccc gagcactacc gcgccgtcgc cgaggtcatc aactacgtct tccgcctcaa
     2401 gggcaagatt aaggcgcgca caggctcctg accaggcgag cccgtctgtg ctaaaagacg
     2461 cgcgtgagcg agagggcaag tgacgcgagc gcggcggcca tgaccatgca accggcgatg
     2521 tcgggcgggg cgctcccttc ggcgacgtcg ccggccgata tgggcatgcc ggcctggacg
     2581 cgctggctcg gcaccatcgc gcttgcgctg gccgccctgg cgctgctcct tttcgccgtg
     2641 tcgctggcgg agttcgtcga aggcgcaacg gcccggccgg tggccgtgat gctgatcgtc
     2701 ttcgcggtga tctgcggcgt ggcggcgctg gtggcgcgca aggtccatgg cgcgctggcc
     2761 ggccgactcg acctcctgag ccaggcgctc gaggcctcgc ccaacgccca tctcatcgtc
     2821 gcgcccgacg gcttgatcgc ctatgccaac gccgccttcc atcgcttctt cccggagctc
     2881 gccgagccgc cgctccaggc gatcgagcgg cgcttcggcg aggacgagcg ctccgccgtc
     2941 gagtttgcgc ggctgcgcag cgaggtcgtg cggggcggcg ccacggcgca attggcgctg
     3001 cgcaacggcg ccggccccgc cacctggttc agcctgtcgg cgcatctcct ggcggggcgt
     3061 ccgggctacg gactctggag cttcgaggat gtgaccacgc gccacgagat ggagcaggtg
     3121 atccgcgacg agcagatgaa gctcgccgac ttcctcgacc acgcgcccac gggcttctac
     3181 tcggtggatg gcgaggggcg cttcctcttc gtcaaccagg cgctcgccga ctggctgggc
     3241 gcgtcgccga cggcgctggt cgagaacggg gcgaagctcc gcgacttcat tatcagcgac
     3301 gtgccgcccg gaacgccgcc tcatgcgccc ttcgaaggcg gcgccgcggg cgccatgcgg
     3361 ggcgaagtgg tgctgcgcgg gcgccagggt cgcaccgtct atgcctcgat cagccagagc
     3421 gcggttcagg tcggcgacgg cctgcgcacg cggtcggtgg tgcgcgacct aacgcccgag
     3481 cgcgaatggg aggaggcgct gcgccagtcg cgccggcgct tccagcgatt ctttgccaat
     3541 gcccccgtgg gcatcgcgct gctcgatcgc accggccgcc tccaggaggc gaaccgggcg
     3601 ctgggcgagc tcttcggcgc gccgccggcc gagctcatcg gcaagacgct ggtgagcttc
     3661 ctgacgcccg aaagccaggg cgaggtggcg gcgaagctcg ccagcgtcgc cgagggtcgc
     3721 cttgcctcgc ggcccgtgga gatccgcctc gccggccccc gcgacaagtc ggcggcgctc
     3781 ttcgtcagcc gcgtcggcgc ggcggaagag ggcgaggccg cggtcatcct gcatttcatc
     3841 gacaccaccg agcaaaagag cctcgaggtg caattcgccc agtcgcagaa gatgcaggcg
     3901 gtgggccagc tcgcgggcgg tgtggcgcac gacttcaaca acctgctgac ggcgatgatc
     3961 ggcttctgcg acctcctgct gctgcgcttc cggccgggcg acccgtcctt cgccgacatc
     4021 atgcagatca agcagaacgc caaccgcgcc gccaacctcg tgcggcagct cctcgcattc
     4081 tcgcgccagc agaccttgca gccgcgcatc ctcaacatca cggacgtgct ggccgagctc
     4141 tcgcatctgc tgcgccgcct catcggcgag aacatcgagc tcaaggtggt gcatggccgc
     4201 gacctcggcc tcgtcaaggt cgaccagggc cagctcgagc aggtaatcat caacctcgcg
     4261 gtcaatgcca gggacgccat gcccggtggc ggcacgctca ccatccgcac cggcaacgtc
     4321 acgagcgagt tcccgatgcg caggggccat gaggtgatgc cgcccggcga gtatgtgcag
     4381 gtcgaggtca ccgacaccgg catcggcatc cccaaggaga atctcgtccg catcttcgag
     4441 ccgttcttct cgaccaagga agtgggctcc ggcaccggtc tcggcctctc gaccgtctac
     4501 ggcatcgtca agcagaccgg cggcttcgtc ttcgtcgaca gcgagcccgg caagggcgcc
     4561 gctttctcga tctacctccc gcgccacgcc ggcgccgcgg ccggcgccgg cgcggaagcg
     4621 ggggcggcgg cgcggtccgg ggccgacctc gccgacgccc aggcgacgcg cgacctcacg
     4681 ggcaccggca cggtgctcct ggtggaggac gaggacccgg tgcggctctt cagcgcgcgc
     4741 gccttgcgca acaagggcta caaggtgatc gaagcgaaga gcggcgaggc cgcgctcgag
     4801 gtcatcacgg gcggcggcga gaaggtggag ctcttgatca ccgacgtcgt gatgccgcgc
     4861 atggacgggc ccagcctggt gaagcgggtg cgcgagctct acccggacat gaaggtgatc
     4921 ttcatctcgg gctacaccga ggacgccttc cgcaagcgcc tcgaccgcga cggcgacatc
     4981 cacttcctgc ctaagccgtt cagcctgaag cagctcgcga cgaaggtgaa agaggtgatg
     5041 ctcgcggcgc cggggtgaga acggcgggag cgggctcgct cacgcgaggt cctgccactt
     5101 aggacgccaa tccgtcagtc tgcgcgcttc gagggtttcg ctcgcttcga aggttttgct
     5161 cccttcgaag gcttcgactt cccggaagtg ttgagggcga tcgcttgccg aaggagcgcc
     5221 ttgaaggcgg acttatcgac ttcttctcct tcgtggatgt cgatcgcgcg gcgcgtgttt
     5281 ccgtcgagac tcgaattgaa cagacgggcc ggatccctga gagacgcgcc cttggcgaag
     5341 gtgagcttca cgacattctt gtaggattcg cccgtgcaga tgatgccgtc atgcgaccaa
     5401 accggagtgc ccatccactt ccactcctcg acgatttcgg ggtcggcttc cttgatgagc
     5461 ttgcgtattc tgccgagggt ttccccgcgc caatccccga gctcggcgat tcttttcgaa
     5521 ataagctccg atgccgaggg tccctggctc gcgtccgatt ttttcatcgc cagatcttga
     5581 ttcgcgcctg catatgcgtt ccccgttcaa gacattcgct tcagtcaatc cgcgccaagg
     5641 cctgctccaa ggccgcaaag aaccgctgcc acccggcctt ggcgccgtgg taggcctgct
     5701 gctgatccgg ccggaagctc gactgctcca tgcgcaggcg ggtccccgcg cccgtgggcg
     5761 tgagagtcca agtgacgaca ctctcgagac cataagccgc ccaggtgtaa gacagcgttt
     5821 tgttcggctc gatcgcgaga acctgacagt cgacagcgcc ccagtccgcg cgcagattga
     5881 aacgatggct cacgacaggc ttgaagtcgt tcttcatgag ccactcctcg atcaggtgtg
     5941 gttgcgtgag cgcgcgccag atcttctccg gcggatgagg tatctcccgt tcgacgacga
     6001 cggagcgcgt ttcggtcgcg ggcttgctca ttggtccatc cttttgagca gatcttcgag
     6061 gtcgtcgaac cggctttgcc agaagccggc catccggctc gtccaatcga tcagcggcgc
     6121 caaggcgccc agctgcgcgc tatagtgcgt ctggcgacct tcgtggcggt cgcgcaccag
     6181 cccggcctgc ttcaggatcc cgagatgctt cgagacggcc ggttgcgaga ccccggcccg
     6241 agccgtcaga gcacccaccg tctgctctcc tttccggcac aatcgctcga aaatagcccg
     6301 ccgcgtcgga tcggcgagcg ttctgaagag cacatcgtga gcattcggca ttttcgatcg
     6361 ataacctgct ggatattgat ttatatataa ccattgagat atgcgtgagt caagcgagca
     6421 cgatggagca gaacgccggc actggcgtgt ccggcgggaa atacgcccgt cggtggcgag
     6481 cagcggccct cgacccaggt cggagggtgg ccgatggcaa gttatgctat cagggatagt
     6541 atcacgatcc cagcagcggc gatggaaaag ccaatcctta cgagaccctt gccgtttcat
     6601 gttccctggt ctatagaata tgcatcaggc ctgtctgggc cgccttcttc gcggagcagc
     6661 aaagcggatt cgtcacgcgg gcttgcatcg cccccggcgg ggccgcgcca tgacactgtt
     6721 catgacattg tcatcgtcgg cggcggggca ggcgggctca agctcgcgac gcgactgggc
     6781 gacacgctcg gccgctcggg gcgcgctcgg atcgccttgg tggaaaagcg ccgcgcacat
     6841 ctctggaaac cgctgctgca tgctgtcgcc gcggggagcc tgaatgtcga cgagcatgcg
     6901 ctcgactatc tggctcaggc gcattggcac catttccgat atcgcctcgg cgagatggtt
     6961 ggggtcgatc gtgcgtcgaa gctcgtgcat gtcgcgcata cgccggacga cgagggtggc
     7021 gagatcacgc cggagcggtg cttccgctac gacacgctcg tgatcgcgac cggcagcgta
     7081 tcgaatgatt tcggaacgcc gggtgcgagc cgccacgcga tcccgctcga cacgcccgaa
     7141 caggcggtgc gtttccatcg ccgtctcgtc aacgcctgca tccgcgccca cacgcaacga
     7201 gggccggtgc gtcccggaca attgcatgtg gcgatcatcg gtgccggcgc cacgggtacg
     7261 gaacttgcgg ccgaacttca tcgcaccacg cgccaggtgg tcggctacgg ccttgatcgc
     7321 atcgatccgg aaaaggacgt gcgcatcacg ctgatcgagg ccgccgatcg tattctgccg
     7381 gtgttgccgc ggcgagtctc cgaagctacc gctcgcctgc tggcgcatct cgctgtcgaa
     7441 gtgcggacca atgcgcgggt gatcgaagtg cgcgaagacg gcgtggtcct tgccgatggc
     7501 agcttggtgc ccgccgagct tgtcgtctgg gcggcgggcg tcaagggtcc cgacgtgttg
     7561 aatcgtctcg acggcctcga gaccaaccgc gcgaaccagc tcgtggtctt gccgacgcta
     7621 cagaccacga gggatcccga tgtcttcgcg atcggcgatt gtgccggctg cccgcgtccc
     7681 gggcacgcca cgcccgttcc gccgcgggct caggctgcac accaacaggc attgcacctc
     7741 gtcaagcagc tcgcgcggcg cgtcgacggc aagtcgaccc acgcctatgt ttatcgcgat
     7801 ttcggcgcgc tggtgtcgct cggccgctat agcacggtcg ggagcttgat ggggttcctc
     7861 atcggcaaaa gccttttcgt cgagggctgg tacgcccgcc tgatttatcg ctctctctat
     7921 acgaggcacg agaccgcgct gcacggcctc ggcaccgtcc tgctcggcat tatggcacgt
     7981 acgatcatcc gtcgcccgca accggtcgtg aagctgcact aaacgacggg ctgagacccg
     8041 atttatgccg ccgctcccgc gtccttcgct cgtcaaatct cgaactggcg gccgatctcg
     8101 acggtggcgt cgctcgggag gttgaggaag tcgctgagct gggcggcgtt ccgctgcagg
     8161 aaggcgaaga gatgctcctg ccagcccggc aagcctttgt ggtcgctgcg gcggagcacg
     8221 gtctcgtgac cgacatagaa cgtgacatcg ctgaggtcga cggcgcagcc caattgcttg
     8281 gcctggcgca acagcgctgg cacgtccggg cgctccatga agccgtagcg cgccgtggcg
     8341 cggaagaaat cgggcgcgat gcgctcgacc gtgacgcgag tcgccgcgtc gacccacggc
     8401 accgacgccg tgacgacctt cagcgccagg agatgccggt gcagcgcccg gttcttccgg
     8461 acgtgccaga gcatcaccgg cggcgcgtcg cgggtggtgc gcgtcaggaa cacggcggtg
     8521 ccgggaacgc gcgcgatgcc ggccgcggta atgcgggcga tgaactggtc ggccggctcg
     8581 gtcatggtca cgaggcccgc cgctaccgcc tgcgtgccgc gatgccaggt cgtgagcacg
     8641 acatagacca gaaccgcgag cgtgagcggg acccagccgc cttccaccac cttcgtcgca
     8701 ttggcggcga agaaggtggc gtcgatgcag aagaagaggc tggcaacggc gaggctcggc
     8761 gcccagttcc agtgccaaac ctcgcgcatc gcgaccagca acaggaccgt cgtcaacagc
     8821 atggtgagcg agacggcgat gccataggcc gccgccagcc ggtcggagtt gccgaagccg
     8881 atggtgagcc cgacggtgac gatcatcagc agccagttga cggtcgcgac atagatctgg
     8941 ccgaagcctt ccgacgaggt ctgggtgatg cggagccgcg ggcaccagcc gagctgtatc
     9001 gcctggcgcg tcatggagaa ggcgccggtg atgatcgact ggctggcgat gatcgtcgcc
     9061 agcgtggcga gaaggatgag cggcaggagc agcgttgtcg ggcagaggcg gtagaagatg
     9121 ttgtcgccga tcggcgcccc ggtcatcacc agcgccg
//
LOCUS       DASYNS010000102         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_648095, whole genome shotgun sequence.
ACCESSION   DASYNS010000102 DASYNS010000000
VERSION     DASYNS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648095"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VGP50_03240"
     CDS             complement(<1..1464)
                     /locus_tag="VGP50_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV8026416.1"
                     /translation="MNDVAPACSIGGYVPMIDGPEKVSGRAKYTADFVEPGALAARIY
                     RSPYAHGEILDVDVSEAARLPGVKAIVTGADCDKTFGVLPVARSEHPLARERVRYKGE
                     PVAAVAAVDDATAAAAVRLIRMKVRELPAYYTARAAAAPGAVALHQARPGNVERDVSF
                     ELGSVAQGFAEAALVRERSYHCAEVCQNQMEMHAALADYDPLRDRMTVHASTQVPYYV
                     HLMLSRILDMDMSRIRVVKPQVGGGFGARTEALNVELIAALLARRAGGRVRLVITREE
                     TFITHRGRPETEIRLKMGMAKDGRITAVECECWQRGGAHSGYGIVTILYSGSMLYAIY
                     DLHNVKYIGRRVLTNTPPCGAFRGHGTVDVRFAFEALLDQMAGELGLDPFAVRRANLL
                     NAPTVTDNDLMVNSYGLPQCLDFVERGSGWKERRGRLGKGKGLGMACSHYISGASKPV
                     NWTGEPHATVKLKLDFDGSIVLLTGAAEIGQGSSTVLV"
     gene            complement(1461..1943)
                     /locus_tag="VGP50_03245"
     CDS             complement(1461..1943)
                     /locus_tag="VGP50_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV8026417.1"
                     /translation="MSRLLTFVLNGRPRVEAVPDNLLLLDFLREVLRLTGTKTGCDGG
                     ECGACTVLVDDRPRLSCLTLAASVEGKRVETVESLVADGTLSAVQRGFHEKLGSQCGY
                     CTPGFVMASEGLLRRNPEPSDAEIRAALGSNICRCTGYVKIIEAVRHAARLKASGEAP
                     "
     gene            2207..2542
                     /locus_tag="VGP50_03250"
     CDS             2207..2542
                     /locus_tag="VGP50_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEV8026418.1"
                     /translation="MEARLKTRLQVAAAVRLCDIHAIPIAIARRGDDDAGTILIKLNQ
                     RERGFTVLAQTRTGMGDLAWLRATGEAPVDEAAADAYIARQVKRDPDLWVIEIEDREG
                     RQIFAGRVL"
     gene            complement(2558..2932)
                     /locus_tag="VGP50_03255"
     CDS             complement(2558..2932)
                     /locus_tag="VGP50_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV8026419.1"
                     /translation="MSKPKRGKSTAAIDQHVGARIRERRIMLGLTQQQLAELIGVTYQ
                     QAHKYERGVNRVSAGRLFQIAKVLSVPMAYFYEGVGEQELKPITSRERMVLEIARNFS
                     GITNERHQEALSQLARVLANGA"
BASE COUNT          488 a         1000 c         1005 g          497 t
ORIGIN      
        1 caccagcacg gtcgacgagc cctggccgat ctcggcagcg ccggtcaaca gcacgatcga
       61 gccgtcgaaa tcgagcttga gcttcaccgt cgcatggggc tcgccggtcc agttgacggg
      121 cttcgacgcg ccgctgatgt aatgcgagca cgccatgccg aggcctttgc ctttgccgag
      181 ccggccccgg cgctctttcc acccgctccc ccgctcgacg aagtcgaggc attgcggcag
      241 gccgtagctg ttcaccatca gatcgttgtc ggtgacggtc ggcgcattca agagattggc
      301 gcgccgcacc gcgaagggat cgagcccgag ctcgcccgcc atctgatcga gcagggcctc
      361 gaaggcaaag cgcacatcga ccgtgccgtg gccgcggaaa gcgccgcaag gcggcgtgtt
      421 ggtgagcacg cgccggccga tgtatttcac attgtgcagg tcatagatgg cgtagagcat
      481 cgagccggaa tagaggatgg tgacgatgcc atagccgcta tgggcgccgc cgcgctgcca
      541 gcattcgcat tcgaccgcgg tgatgcgccc gtccttggcc atccccatct tgaggcggat
      601 ctcggtctcg ggccggccgc gatgggtgat gaaggtctct tcgcgggtga tgacgaggcg
      661 cacgcgaccg ccggcgcgcc gcgccagcaa ggcggcgatg agctccacat tgagcgcctc
      721 ggtgcgggcg ccgaagccgc cgccgacctg cggcttcacc acgcggatgc gcgacatgtc
      781 catgtcgaga atgcgcgaca gcatgagatg cacgtaataa ggtacctgcg tgctggcatg
      841 gacggtcatg cggtcgcgca gcggatcgta gtcggcgagc gcggcatgca tctccatctg
      901 gttctggcac acctcggcgc agtgatagct gcgctcgcgc accagcgcgg cctcggcgaa
      961 accctgggcg acgctgccga gttcgaagga gacgtcgcgc tcgacattgc cggggcgggc
     1021 ctggtgaagg gcgacggcgc cgggtgccgc cgccgcgcgc gccgtgtaat aggcgggaag
     1081 ttcccgcacc ttcatgcgga tgagccgcac tgccgcggcc gcggtcgcgt cgtcgacggc
     1141 tgcgaccgca gccacgggct cgcccttgta gcggacgcgc tcgcgcgcca agggatgctc
     1201 cgagcgcgcc accggcagca cgccgaaggt cttgtcgcaa tcggcgccgg tgacgatcgc
     1261 cttgacgccg ggcaggcgcg ccgcttccga gacatcgaca tcgaggattt cgccatgggc
     1321 gtaagggctg cgatagatgc gcgcggcgag cgcacccggc tcgacgaagt cggcggtgta
     1381 tttggcgcgg cccgagacct tctccgggcc gtcgatcatc ggcacgtagc cgccgatcga
     1441 gcacgcgggc gcgacgtcgt tcacggcgcc tcgccgctcg ccttgaggcg tgcggcgtgg
     1501 cgcaccgcct cgatgatctt cacatagccg gtgcagcggc agatgttact gcccagcgcc
     1561 gcgcggatct cggcgtcgct cggctccggg ttgcgccgca gcagcccttc ggacgccatg
     1621 acgaatccgg gcgtgcaata gccgcattgc gagccgagct tctcgtgaaa gccacgctgc
     1681 accgccgaca gcgtcccgtc ggccaccagc gattcgaccg tctcgacacg cttgccctcg
     1741 acgctcgccg ccagcgtcag gcaggagagc cgcggccggt cgtcgaccag caccgtgcag
     1801 gcgccgcatt cgccgccgtc gcatcccgtc ttggtgccgg tgagacggag cacctcgcgc
     1861 aggaaatcga gcaacaggag attgtcggga acagcttcga cccgaggacg cccgttgagc
     1921 acgaaagtga gaagcctgct cacgacgtta cgcccgcaag attgttttag tcactgatca
     1981 tttgtatatc atacaaccgc cgccgacgca aggccggcgt tcggtcggaa gggcgcgccg
     2041 cgtccgaatc tccttttagg gtcagggccc tagcttaggg tcggcgcccc ggagggagac
     2101 catcgctggc agtcggtcgc acggcggcgg gcgggctcag caaagcgcgg gacctgctgc
     2161 gcggccgcat tgatcccggc gccgcgatgg gcgatatctt gctctcatgg aggcgcggct
     2221 caagacgcga ttgcaggtgg cggcagcggt gcgcctctgc gacatccatg cgattcccat
     2281 cgccatcgcg cggcggggcg atgacgatgc cggcacgatc ctgatcaagc tcaaccagcg
     2341 cgagcgcggc ttcacggtgc tggcgcagac gcgcaccggc atgggcgacc tcgcctggct
     2401 gcgcgcaacc ggcgaggcgc ccgtcgacga ggccgcagcc gacgcttata tcgcccgcca
     2461 agtgaagcgc gatcccgacc tctgggtgat cgagatcgag gatcgcgagg ggcgtcagat
     2521 cttcgccgga cgagtcctgt agcgaccgcg gcgcggctca ggcgccgttc gcgagcacgc
     2581 gcgccagttg actgagcgct tcctgatgac gctcattggt gatgccggaa aagtttcgcg
     2641 cgatctcgag caccatgcgt tcgcgcgacg tgatcggctt cagctcctgc tcgccgacgc
     2701 cctcgtagaa ataggccatc ggcacgctca gtactttggc gatctggaac agccgcccgg
     2761 cggagactcg attgacgccg cgctcgtatt tgtgcgcctg ctggtatgtc acgccgatga
     2821 gttccgcaag ctgctgctgc gtcaatccca gcatgattcg ccgctcgcgg atgcgcgctc
     2881 cgacatgctg atctatcgcg gcggttgatt ttcctcgctt cggtttcgac attagggtca
     2941 cccgatgctc cattgttgcg gcaatccctc gccccgccac cggcacaaga
//
LOCUS       DASYNS010000103        19694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_492558, whole genome shotgun sequence.
ACCESSION   DASYNS010000103 DASYNS010000000
VERSION     DASYNS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492558"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3275)
                     /gene="dnaE"
                     /locus_tag="VGP50_03260"
     CDS             complement(<1..3275)
                     /gene="dnaE"
                     /locus_tag="VGP50_03260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV8026420.1"
                     /translation="MTHADFIHLRTHSAYSLSAGAIKVKALVGLAKRHGMPALALTDA
                     GNLFGALEFAQAASDAGIQPIIGCELGLRRSDGEANGWSAKLGPAPAPDKVILLAQNE
                     LGYRNLLKLVSKSFLETTGGEAPQLDLGAFAGQSDGLLLLTGGPAGPIGRLLAEGQGP
                     AAEALAQRLAALFPGRLYVELMRHGLPAEDRIEGALIDLAYRHGLPLVATNAVFFADP
                     DLYDAHDVLLGIAEGTVVSAPGRQRLTPHHHFKSPASMRLLFADVPEAVDNTLVIARR
                     CAFMPEPRKPILPPFPTQSGRGENEELRAMAAEGLERRLAAQIFGDGMAETERAAAAR
                     PYRERLDFELDVIIKMGFAGYFLIVAEFIQWAKAQGIPVGPGRGSGAGSVVAWSLTIT
                     DLDPLRFGLLFERFLNPERVSMPDFDIDFCQDRRDEVIRHVQEKYGRDRVAQIITFGT
                     LQARAVLRDVGRVLEMPYGQVDRISKLVPYNPANPVKLQQAIDAEPQLQQLRDGDESV
                     ARLITIALKLEGLYRHASTHAAGVVIGDRPLDELVPLYRDPRSDMTVTQFNLKYVEVA
                     GLVKFDFLGLKTLTILARAVELLGQRGVSLDLSTLPLDDAATFALLTRGDTVGVFQVE
                     GQGMREVLKRMRPTRFEDLIALVALYRPGPMDDIPKYLAAKHGEEQPDYLHPSLEPIL
                     RETYGVMVYQEQVMQIAQVLSGYTLGGADLLRRAMGKKIKSEMEAQRRNFIDGATSRG
                     VSATQAELVFEKVEKFAGYGFNKCHSAPYALIAYQTAYLKANHPVEFMAASMTLDLGN
                     TDKLNVFRGECDRLGIKLLPPDINRSEVAFAVEQTPQGAAIRYALAAVKGVGAQAMRE
                     LVEERNAKGPFRDLFDLARRLDVKSFNRRQFEALVKAGAIDCLNRNRAQSFAAAEMLL
                     RYAASAAEDRQSRQVSLFGDGDEPARNPALPVIQDWPPIERLQQEFDAIGFYLSSHPL
                     DAYGKSLERLGVVRFAELPARLAGGGATRYKLAGIVVGKKERTSARGNRFAFVQMSDS
                     TGVFEVTLFSEVLSQSRQLLEAGQKLLVTTDVRAEEETLRLTAQRIEALDSMVAHAAA
                     GLKV"
     gene            complement(3272..3994)
                     /locus_tag="VGP50_03265"
     CDS             complement(3272..3994)
                     /locus_tag="VGP50_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8026421.1"
                     /translation="MNDRRGAPALELRGVGRTYKQAGVSLPVLRGVSVALWPGEIAAL
                     VGPSGAGKSTLLHTAGLLERPDEGQVLLAGQECGGMSDGQRTGLRRAQLGFVYQFHHL
                     LPEFSALENVMMPQMIAGESKAKARARARELLAMVGLADRAEHRPARLSGGEQQRVAV
                     VRALANSPRVLLGDEPTGNLDIHTADEVMGALIDLVRKTGLAALIATHNLDLARRMDR
                     ILSLEDGAIVERTEPRRVEAGR"
     gene            complement(3987..5231)
                     /locus_tag="VGP50_03270"
     CDS             complement(3987..5231)
                     /locus_tag="VGP50_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEV8026422.1"
                     /translation="MAFDTFERMVAFRYLRPRRQEGFISVIAVFSLLGIMLGVATLII
                     VMSVMNGFRAELLHEILGLNGHLGIVAGPNNLRNFDAAAEQVKSVPGVVAVHPLVEGQ
                     VLASAHGQSSGILVRGMRESDLAAEALLAQKIAPDALAKFTDDNVLVGSKLALKLGLS
                     PGDAVTFFSPNSQPTAFGNVPRVKTYHVAGTFEVGMYQYDSNLVLMPLPAAQLFFQTS
                     DAATSLEVFVDDPDHSIAIGYAIQAKLGNGVGIVDWQRSNQAILGAVQIERDVMFIIL
                     SLIVLVAAFNIISSMIMLVKDKTRDIAILRTMGATRGRILRIFILSGASIGVIGTIAG
                     FVLGIAFTTHIEELRQFLQHVLGVTLFNEQIYFFTRIPAKIDPQEIISVLAMAFGLSF
                     LATLYPSWRAARLDPVEALRYE"
     gene            complement(5231..6547)
                     /locus_tag="VGP50_03275"
     CDS             complement(5231..6547)
                     /locus_tag="VGP50_03275"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV8026423.1"
                     /translation="MRLSAYFLPLLRETPSEAQIVSHRLMLRAGMIRQSSAGIYSWLP
                     LGLRVLKKVEQIVREEQDRAGALEVLMPTIQPAELWQESGRYEDYGKEMLRITDRHER
                     TMLYGPTNEELITDIFRHSVKSYRDLPKNLYHIQWKFRDEVRPRFGVMRGREFLMKDA
                     YSFDLNAKGAKHSYNKMFFAYLRSFARMGLKAIPMRADTGPIGGDLSHEFIILADTGE
                     SQVFCDRAWLDSDVLDMGVDYANDLEPQFRQWTALYAATDEKHDAKSCAVPPERLVSA
                     RGIEVGHIFYFGTKYSKPMGAVVAGPNGEQITVEMGSYGIGVSRLVGAIIEASHDDAG
                     IIWPEAVAPFKVGLINLRANDAKCAAAADDLYARLSRAGIDVLYDDRDESPGAKFAAM
                     DLIGLPHQLVLGPRGLEKGVVEVKDRRGSARQELAPADALARLGVS"
     gene            complement(7071..7919)
                     /locus_tag="VGP50_03280"
     CDS             complement(7071..7919)
                     /locus_tag="VGP50_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEV8026424.1"
                     /translation="MLLRASVALADASSATMANIPDPEDGSVAAGAYVNNYFGLSYAL
                     PPGWTEGLKGPPPSYTGYYVLASLDSRSLGQPSLLVVAQDMFFGAKPMITAKQAAGEL
                     RDAEAQVPKMTIDLEPAETRIAGRSFMRVDYSAGGLYRAWFATELRCHLVIFNFTTTD
                     TEALQSAVRSLDKMILPSEADSGGDAGTTAPVCIHDYASATNIVHRVEPAPAGPRFLK
                     IPVRIVIGTDGAVKHIHVISAFPEQKTAIETALAQWRFKPYEVQGHPAEVETGLIFEF
                     KPERNN"
     gene            complement(7970..8155)
                     /locus_tag="VGP50_03285"
     CDS             complement(7970..8155)
                     /locus_tag="VGP50_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026425.1"
                     /translation="MGGDLCRAAILGHAGDPSHAVGAASSPRPGVKAPVTTGIAAVLS
                     VVLYLVAEADLISFRPS"
     gene            8437..9804
                     /locus_tag="VGP50_03290"
     CDS             8437..9804
                     /locus_tag="VGP50_03290"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEV8026426.1"
                     /translation="MTTVSAIALLLPLATHKAHAQAAAGVSDPGVRGGPAGAGGALPG
                     LNSDELNFFFQTRARFQEVDSVQGTIADVDPGLPTPETGGGLGPRFNGNACAVCHIQP
                     AVGGSSPTTNPQIRLATLDGATNTIPSFISNTSPVREARFITNPNGTPDGGVHDLFVI
                     TGRTDAQGCNILQPNFPQAVAANNVIFRIPISVNGDGLVENTQDSALLADAQTAPDGP
                     ASLGITVGFFNTNGNDGTATKFGWKAQNKSLLLFSGEAYNVEQGVTNELFNNERDETQ
                     GCVFNPTPEDATALENTRNSNSPASDFSSDIVNFAGFMRLSAPPAPAASSNATVQGSN
                     QFVNIGCAACHVTTHTTAASIFTNQSNVAFHPFSDFALHNMGTGLQDQVSQGAANGQQ
                     FRSAPLWGVGKRIFFLHDGRTNNLVTAIEAHSSSGSEANQVIANFNKLSASDQQDIIL
                     FLRSL"
     gene            complement(9880..10116)
                     /locus_tag="VGP50_03295"
     CDS             complement(9880..10116)
                     /locus_tag="VGP50_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEV8026427.1"
                     /translation="MTWVQAIVAYVIIWWVVIFAVLPFGVRPVEKGDLGHAVGAPSNP
                     RLGIKAAVTTVIAAVLWLVLYAVTRSDLVSFRPA"
     gene            complement(10127..10531)
                     /gene="mce"
                     /locus_tag="VGP50_03300"
     CDS             complement(10127..10531)
                     /gene="mce"
                     /locus_tag="VGP50_03300"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEV8026428.1"
                     /translation="MIGKLNHVAIVVPDVRKAATVYRETLGARVSDPVSLPPHGVTVV
                     FVTLDNTKVELLEPYGPDSPVSNFLARNPSGGMHHVCYEVADIIAARDKLKAEGARVV
                     GDGEPKIGAHDKPVLFLHPKDFCGTLVELEQV"
     gene            complement(10543..12210)
                     /locus_tag="VGP50_03305"
     CDS             complement(10543..12210)
                     /locus_tag="VGP50_03305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV8026429.1"
                     /translation="MSNTTPNRPDELVFLALGGVGEIGMNLSLYGYGGRWLMIDCGIS
                     FADDTLPGVDVIMPDPGFIAERAADLAGLIVTHAHEDHIGAIPYLWPRLRCPVYATPF
                     TAAVLRLKLVEAGLEHQVPIIEVPMSGKFTVGPFEIELVTLTHSIPEPNAVVLRTPLG
                     TVLHTGDWKFDRDPLVGELSDMAALRRLGEAGVLAMVCDSTNALRPGESGSEADVRKS
                     LIELVGKCRNRVVIACFASNVARLESAAAAAAAHDRHAALVGRSLWRIEHAARETGYL
                     EGLPPFLTDEEASYLPRDKVLLVCTGSQGEPRSALARIAEDDHPEIVLEEGDTVIFSS
                     RVIPGNERAIGRLQNRLAGLGIELITEHDHLVHVSGHPAQDELTRMYQMVRPRIAVPV
                     HGETRHLLAQARLAEQCQVPQTIVTRNGEVVKLAPGPAAVIGEVSTGRLVVDGTRLLP
                     AGSEALRSRQRMTFNGAALATIVVDGEGRLLAPPQVTVQGLGEPEQDAALTEELRQKV
                     EAALAELGPRERRDDAALREAARLAVRRSLRAWHGKKPVTEIHLVRI"
     gene            complement(12207..12980)
                     /locus_tag="VGP50_03310"
     CDS             complement(12207..12980)
                     /locus_tag="VGP50_03310"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEV8026430.1"
                     /translation="MLLAINANNTNTVFSVWEGDQLKGAWRAATDARRTADEYVVWLD
                     HLLALEGLSRNGLTGAIIGSVVPDANFNLVTLCRRYCNSDPLVVGEPGVVLGTKALVD
                     RPEEVGADRLLNTVAAHDRYKGPLVVVDFGTATNFDVVDKDGNYCGGVIAPGVTLSIT
                     ALHMAAAKLPSVRLRRIDHVIGKDTVACMQSGVFWGYVGLVEGLVARIKKEFGAPMGV
                     IATGGLVPLFEGAIPGIDRVDPNLTLWGLRLVYERNRKP"
     gene            complement(12999..13757)
                     /locus_tag="VGP50_03315"
     CDS             complement(12999..13757)
                     /locus_tag="VGP50_03315"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEV8026431.1"
                     /translation="MTATPRLPPFFRLLSVAELDSTNEEAKRQAAQGAPAGLLVWAGA
                     QTAGRGRRGRSFVSPPGNLYMSILLRPDHAAAQAAQLGFAAALAVGEAARSFLPAGTK
                     LAYKWPNDVLVEGRKASGILLESQGAAGGRLAWLVVGIGVNLASYPENVETPAISLRA
                     AGADAATPETMLEAVAGRFYHWHERWRVEGFPPLRAAWLAGADGLGDDIRVRLQGSET
                     RGRFAGLDEEGALLLEEGTAQRRITAGDVFPAGA"
     gene            complement(13754..15208)
                     /gene="nuoN"
                     /locus_tag="VGP50_03320"
     CDS             complement(13754..15208)
                     /gene="nuoN"
                     /locus_tag="VGP50_03320"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEV8026432.1"
                     /translation="MTADALPSFLPALPEIFLAASAMALLLLGAFQHAAYATREITWL
                     SVLVLAITAVLVAASGFERQVTFYGMFFTDGFAVFMKILVLIGSLLSIILSMRFNERE
                     QIERFEYPVLVLLATVGMMVMISANDLISLYLGLELQNLALYVIASFQRDSERSTEAG
                     LKYFVLGALSSGMLLYGASMVYGFAGSTNFDTLAHSLDTSDGYSIGLVIGIVFIAVGL
                     AFKVSAVPFHMWTPDVYEGAPTPATAFFSVAPKIAAMALFIRVMTYPFGHLDDQWRQI
                     IWVIAVGSMLLGAFAAINQRNIKRLMAYSSIGHVGYMLIGLAATSETGVRGILIYLAI
                     YLFMNVGTFAVILCMRRQGRAVEGIDDLAGLSRTHPGLAAALAIFMFSMAGIPPLGGF
                     FAKFYIFMSAINSGLYSLSVIGVVTSVVAAYYYLRIVKIMYFDEPTAAFDQPFGGEMQ
                     GLLAFTAIAILLFAFFLSPIAGAAAAAAGALFPG"
     gene            complement(15205..16740)
                     /locus_tag="VGP50_03325"
     CDS             complement(15205..16740)
                     /locus_tag="VGP50_03325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEV8026433.1"
                     /translation="MEHWPLLSLVTFLPLAGVLLIVVLRGESEAVARNARWIALWTSL
                     IDFALSLLLWGYFDPASFKFQFVEHVDWLPDFNIAYHLGIDGISLFFVLLSTFLTPVC
                     VVASWTSVTRRVKEYMIAFLVLETMMVGMFSALDFVIFYVFFEGVLIPMFLIIGVWGG
                     PRRVYAAFKFFLFTLLGSVLMLLAILAIYSYVGSTDIVEALDRLPVILKDAPRVQTWL
                     WLAFFASFAVKVPMWPVHTWLPDAHVEAPTAGSVILAGVLLKMGGYGFIRFSLPMLPL
                     ASHDLATWVFVLSVVAIIYTSLVALAQEDMKKLIAYSSVAHMGFVTMGTFAMTSEGLQ
                     GAMVQMLSHGIVSAALFLCVGVVYDRLHTREIARYGGLADNMPKYALVFMVFMLASVG
                     LPGTSGFVGEFLSLIGTFQVNTAVTFFATTGIILGATYMLYLYRRIIFGTITREDVRA
                     MLDLNWREVAIFAPMIAIVLWMGVYPSSFLRPIQPSVANLVERVQAAVRDEAAAKLAE
                     AGP"
     gene            complement(16746..18698)
                     /gene="nuoL"
                     /locus_tag="VGP50_03330"
     CDS             complement(16746..18698)
                     /gene="nuoL"
                     /locus_tag="VGP50_03330"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEV8026434.1"
                     /translation="MTIAAVFLPLLGAFVAGLFGRWIGDRGAQLVTCTSLVLAAICGV
                     ILFVEVSVGGQARTTVDLLTWIDTGDLKAQWALRFDSLSAVMVAVVTVVSAMVHIYSV
                     GYMAHDHSIPRFMAYLSLFTFFMLMLVTADNFLQLFFGWEGVGLSSYLLIGFWYDRPS
                     ANAAAIKAFVVNRIGDFGFLLGIFAVYALFGSLHFGTVFDAAPNFAHAPFNFLGFKVD
                     ALTVTCILLFVGAMGKSAQIGLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVARLSP
                     LFEYAPVALAVVCFIGATTAIFAASVGVAQNDIKRVIAYSTCSQLGYMFIAAGVSAYS
                     AAIFHLMTHAFFKALLFLGSGSVIHAMSGEQDMRKMGGLWRLIPVTYALMWIGSLALA
                     GVGIPGVFGFAGFYSKDVILEAAWGAGSGVALYGFWLGVLAAFLTAFYSWRLIFLTFH
                     GAPRASQDVMRHVHESPQVMLAPLYVLAAGAVFAGVLGYNFFVGEGREAFWGDSIVVL
                     PEHASLEAAHHAPFWVGTLPVIVGLFGIAVAWVAYIWRPELPALAARRLRPVYLFLLN
                     KWYFDELYDFLLVRPAMRLGRFLWQSGDGRTIDRFGPDGLAAVSVRLAVRASRLQTGY
                     LYQYAFVIVIGVVAFVSYSILRRAFW"
     gene            complement(18853..19164)
                     /gene="nuoK"
                     /locus_tag="VGP50_03335"
     CDS             complement(18853..19164)
                     /gene="nuoK"
                     /locus_tag="VGP50_03335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEV8026435.1"
                     /translation="MLEIGLTHYLVLAAIIFTLGIFGIFLNRKNVIIMLMSIELMLLA
                     VNINFVAFSAYLHDLVGQIFAMFVLTVAAAEAAIGLAILVVYFRNRGTIAVQDIHLMK
                     G"
     gene            complement(19167..>19694)
                     /locus_tag="VGP50_03340"
     CDS             complement(19167..>19694)
                     /locus_tag="VGP50_03340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEV8026436.1"
                     /translation="HSVLFLILAFFNAAALFLLLGAEFLALILVIVYVGAVAVLFLFV
                     VMMLDIDFAELRRGFITYLPIGALIGLMLFLELFLAFAAWTVAPDVAKSIAAPTPALS
                     GLTNTQALGALIYTRYVYAFQTAGMVLLVAMVGAIVLTLRAREGVRRQKIAVQLARRR
                     QETLAIVKVQPRQGI"
BASE COUNT         3734 a         6337 c         6604 g         3019 t
ORIGIN      
        1 actttgagcc cggccgcggc atgggcgacc atgctgtcga gcgcctcgat gcgctgcgcc
       61 gtgaggcgaa gggtctcctc ctcggcgcgc acatcggtcg tcaccagcag cttctgtccg
      121 gcctcgagga gctgccggct ctggctcagc acttcggaga agagcgtcac ctcgaagacg
      181 ccggtcgaat ccgacatctg cacgaaagcg aagcggttgc cgcgcgcgga ggtgcgctcc
      241 ttcttgccga cgacgatacc ggcgagcttg tagcgtgtgg caccgccgcc ggcgagccgt
      301 gccggcagct cggcgaagcg aaccacgccg agccgctcga gactcttgcc ataggcgtcg
      361 agcggatggc tcgagagata gaagccgatg gcgtcgaatt cctgctggag ccgctcgatc
      421 ggcggccagt cctggatgac cggcagcgcc gggttgcgcg ccggctcgtc gccatcgccg
      481 aagaggctca cctgccggct ctggcggtcc tcggcagcgc ttgcggcata gcgcagcagc
      541 atctcggccg cggcgaagct ctgggcgcga ttgcggttga ggcagtcgat cgcaccggcc
      601 ttgaccagcg cctcgaactg gcggcggttg aagctcttga cgtcgagccg gcgcgccagg
      661 tcgaacaggt ccctgaacgg ccccttggca ttgcgctcct cgacgagttc gcgcatcgcc
      721 tgggcgccca cgcctttgac ggccgcgagc gcgtagcgga tcgccgcgcc ttgcggcgtt
      781 tgctccaccg cgaacgcgac ctccgaacga ttgatgtcgg ggggcagcag cttgatgccg
      841 aggcgatcgc attcgccgcg gaagacattg agcttgtcgg tgttgccgag atcgagcgtc
      901 atcgacgccg ccatgaactc gaccggatgg ttggccttga gataggccgt ctgatacgcg
      961 atcagcgcgt aaggcgcgga gtgacacttg ttgaagccgt agccggcgaa tttctccact
     1021 ttctcgaaga ccagttccgc ctgcgtcgcg ctcacgccgc gcgacgtcgc cccgtcgatg
     1081 aagttccgcc gctgcgcctc catctccgac ttgatcttct tgcccatggc gcggcgcagg
     1141 agatcggcgc caccgagggt gtagcccgag agcacctggg cgatctgcat cacctgctcc
     1201 tggtagacca tgacgccgta ggtctcgcgc aggatcggct cgagcgaggg atggagatag
     1261 tcgggctgct cttcgccatg cttggcggcg agatatttcg gaatgtcgtc catcggaccc
     1321 ggtcgataga gcgccacgag cgcgatcaga tcctcgaagc gggtcggacg catgcgcttc
     1381 aacacctcgc gcatgccctg gccttcgacc tggaacacgc ccaccgtatc gccgcgcgtc
     1441 aagagggcga atgtggccgc atcgtcgagc ggcagcgtcg agagatcgag actgacgccg
     1501 cgctgcccga ggagctccac cgctctcgcc aggatggtga gcgttttgag gccgaggaag
     1561 tcgaacttca ccagccccgc gacctcgacg tatttgagat tgaactgcgt caccgtcatg
     1621 tcggagcgcg ggtcgcgata gagcggcacc agctcgtcga gaggacggtc gccgatcacg
     1681 acgcccgccg catgggtcga agcgtggcga tagaggcctt cgagcttgag cgcgatggtg
     1741 atcagccgcg caaccgattc gtcgccgtcg cgcagttgct ggagctgcgg ctcggcgtcg
     1801 atcgcctgct gcagcttcac cgggttcgcg gggttgtacg gcacgagctt cgagatgcgg
     1861 tcgacctgcc cgtacggcat ctccagcacg cgaccgacgt cgcgcagcac ggcccgcgcc
     1921 tggagcgtgc cgaaggtgat gatctgcgcc acgcggtcgc ggccgtactt ctcctggacg
     1981 tgacggatca cctcgtcgcg ccggtcctgg cagaaatcga tgtcgaaatc cggcatcgac
     2041 acgcgctcgg gattgaggaa gcgctcgaac agcaggccga agcgcaaggg atcgagatcg
     2101 gtgatggtga gcgaccacgc caccaccgag cccgcgcccg agccgcggcc cgggccgacc
     2161 ggaatgcctt gcgccttcgc ccactggatg aactccgcga cgatgaggaa gtagcccgcg
     2221 aaacccatct tgatgatgac gtcgagctcg aaatcgaggc gctcgcgata gggccgggcg
     2281 gcggcggcgc gctcggtctc ggccatgccg tcgccgaaga tctgcgccgc gaggcgacgc
     2341 tccagcccct ccgccgccat ggcgcgcagc tcctcgttct cgccgcggcc gctctgggtc
     2401 gggaagggcg gcagaatggg cttgcgcggc tccggcatga aggcgcagcg ccgggcgatg
     2461 accagcgtgt tgtcgacggc ttccggcaca tcggcgaaaa gcagccgcat cgatgccggc
     2521 gacttgaaat ggtggtgcgg cgtcagacgc tgccggcccg gcgcgctcac gaccgtgccc
     2581 tcggcgatgc cgagcagcac gtcatgcgcg tcgtagaggt cgggatcggc gaagaagacg
     2641 gcgttggtgg cgacgagcgg cagcccgtgg cgataggcca ggtcgatcag cgccccctcg
     2701 atgcggtcct cggcaggaag cccatgccgc atcagctcga cgtagaggcg cccagggaac
     2761 agcgccgcca ggcgctgcgc caacgcttcg gccgcgggcc cttgcccttc cgcgagcaac
     2821 cggccgatcg gaccggccgg cccgccggtc agcagcagca agccatcgct ctgtccggcg
     2881 aaggcgccca aatcgagctg cggcgcctcg ccaccggtgg tctcgaggaa ggatttgctc
     2941 acgagcttca gcagattgcg atagccgagc tcgttctgcg ccagcaggat caccttgtcc
     3001 ggagcgggcg ccgggccgag cttcgccgac cagccattcg cctcgccgtc gctccgccgc
     3061 aagcccagct cgcagccgat gatcggctgt atcccggcgt cgctcgcggc ctgcgcgaac
     3121 tcgagagcgc cgaagaggtt gcccgcgtcg gtgagcgcca gcgccggcat cccgtgccgc
     3181 ttcgccagcc ccaccagcgc cttgaccttg atcgcccctg ccgagagcga ataagcggaa
     3241 tgcgtgcgga gatggatgaa gtcggcgtgg gtcatcgccc cgcctccacg cgcctcggct
     3301 ccgtgcgttc gacgatggcg ccgtcctcga gcgacaggat gcggtccatg cgccgcgcga
     3361 gatcgagatt atgcgtggcg atgagggccg cgagcccggt cttgcgcacg aggtcgatga
     3421 gcgcgcccat cacctcgtcg gcggtgtgga tgtcgagatt gccggtaggc tcgtcaccga
     3481 gcagcacgcg cggcgaattg gcgagcgcac gcacgaccgc gacgcgctgc tgctcgccgc
     3541 ccgagaggcg cgccgggcgg tgctcggcgc ggtcggcaag gccgaccatg gcgaggagct
     3601 cgcgcgcccg cgcccgcgcc ttcgccttcg actcccccgc gatcatttgc ggcatcatca
     3661 cattctcgag cgcggagaac tccggcagca ggtgatggaa ctgatagacg aagccgagct
     3721 gggcgcgccg gagcccggtg cgctgcccgt ccgacatgcc gccgcactcc tggccggcga
     3781 gcagcacctg cccctcatcg ggccgctcga gcagccccgc ggtgtggagc aaggtcgact
     3841 tgccggcgcc cgaggggccg accagcgccg cgatctcgcc cggccacagc gccaccgaga
     3901 cgccacgcag caccggcagc gacacgcccg cctgcttgta ggtccgcccc acgccccgca
     3961 gctcgagcgc gggagcgccg cggcgatcat tcatagcgga gcgcctcgac cggatcgagg
     4021 cgcgccgccc gccaggacgg atagagcgtc gccaggaagg aaaggccgaa ggccatggca
     4081 aggacggaga tgatttcctg cggatcgatc ttggccggga tgcgcgtgaa gaagtagatc
     4141 tgttcgttga agagggtgac gcccagcacg tgctgcagga actgccgcag ctcctcgata
     4201 tgggtcgtga aggcgatgcc gaggacgaag ccggcgatgg tgccgatcac gccgatcgag
     4261 gcgccgctca ggatgaagat gcgcaagatc cggccgcgcg tcgcgcccat ggtgcgcagg
     4321 atggcgatgt cgcgcgtctt gtccttcacc agcatgatca tgctggagat gatattgaag
     4381 gcggccacca ggacgatgag cgagaggatg atgaacatca cgtcgcgctc gatctgcacc
     4441 gcgcccagaa tcgcctgatt gctgcgctgc cagtcgacga tgccgacgcc gttgccgagc
     4501 ttcgcctgga tggcgtagcc gatggcgatc gagtgatcgg gatcgtcgac gaagacctcc
     4561 aggctggtcg ccgcatcgga cgtctgaaag aagagctgcg ccgccggcaa gggcatgagc
     4621 acgaggttgc tgtcgtattg atacattccc acttcgaagg tgcccgcgac atgatacgtc
     4681 ttgacgcgcg gcacattgcc gaaggccgtg ggctggctgt tgggcgagaa gaaggtcacg
     4741 gcatcgcccg gcgagagtcc gagcttcagg gcgagcttgc tcccgacgag cacgttgtcg
     4801 tcggtgaatt tggcgagagc gtcgggcgcg atcttctggg ccagcagcgc ttccgcggcg
     4861 aggtcgctct cgcgcatgcc gcggacgaga atgccgctcg actggccatg ggcgctggcg
     4921 agcacctgcc cttcgaccag ggggtgaacg gcgaccacgc ccggcacgct cttcacctgc
     4981 tccgcggcag cgtcgaaatt gcgcaggttg ttcggaccgg cgacgatgcc gagatggccg
     5041 ttgaggccga ggatctcgtg cagcagctcg gcgcggaagc cgttcatcac cgacatgacg
     5101 atgatgagcg tggcgacgcc cagcatgatg ccgagcagcg agaagacggc gatgacggag
     5161 atgaaccctt cctggcggcg cggccgcaga tagcggaacg ccaccatgcg ctcgaaggtg
     5221 tcgaaggcca tcaggagacc ccgagccgcg ccaatgcgtc cgccggcgca agctcctggc
     5281 gcgcgctacc gcgccggtcc ttcacctcga ccacaccctt ctcgaggccg cggggtccca
     5341 gcacgagctg atggggcagt ccgatcaggt ccatcgccgc gaatttggcg ccgggcgatt
     5401 cgtcgcggtc gtcatagagc acatcgatgc cggcccggct cagcctggcg tagagatcat
     5461 cggcggcggc ggcgcatttc gcatcattcg cgcgcagatt gatgagcccc accttgaagg
     5521 gcgcgacggc ctccggccag atgatgccgg catcgtcatg gctcgcctcg atgatggcgc
     5581 cgacgaggcg cgagacgccg atgccgtagg agcccatctc gacggtgatc tgctcgccat
     5641 tggggccggc gacgacggcg cccatgggct tcgagtattt cgtgccgaaa tagaagatgt
     5701 ggccgacttc gatgccgcgc gcgctgacga ggcgctcggg cggcaccgcg caggatttcg
     5761 cgtcgtgctt ctcgtcggtc gcggcgtaga gcgcggtcca ttggcggaat tgcggctcga
     5821 gatcattcgc atagtcgacg cccatgtcga ggacgtcgga gtcgagccag gcgcggtcgc
     5881 agaacacctg ggactcgccg gtgtcggcca ggatgatgaa ttcgtggctg agatcaccgc
     5941 cgatcgggcc ggtgtcggca cgcatcggga tcgccttgag gcccatccgc gcaaagctgc
     6001 gcagataggc gaagaacatc ttgttgtagg aatgcttggc gcccttggca ttgagatcga
     6061 aggaataggc gtccttcatc aggaactcgc gtccacgcat gacgccgaag cgcggccgca
     6121 cttcgtcgcg gaacttccac tggatgtggt agaggttctt tggcaggtcg cggtagctct
     6181 tgacgctgtg gcggaagatg tcggtgatga gctcctcgtt ggtcggccca tagagcatcg
     6241 tccgctcgtg gcggtcggtg atgcgcagca tctccttgcc gtaatcctcg tagcgcccgc
     6301 tctcctgcca gagctcggcc ggctgaatgg tcggcatcag cacctcgagc gcgcccgccc
     6361 gatcttgctc ctcgcgcacg atctgctcga ccttcttgag gacgcgcaag cccaggggca
     6421 gccaggaata gatcccggcg ctcgactggc ggatcatgcc ggcccgcaac atcaggcgat
     6481 gcgacacgat ctgggcttcg ctcggcgtct cgcgcaagag cggcaggaaa taagcggaaa
     6541 gacgcatggg acctcggtca acgcccggag cgcgcggcgg cgactttagg caaggccggg
     6601 cgtgatgtcc acgcgcgggc tttcgcgggg cggcgcggcc tcgccgatcc accgccccgc
     6661 atgcggcggc caatgaccgc tcgccgggag accggggcct tgacagcgag gacttcagtt
     6721 gcgagagtaa tggcattcaa catccctgaa caatggcgga gcggcttttg gctcctcgat
     6781 tttgccgaca tcaccccttt tggttgatgc gccctgcgct ttttgcgcag aattttcgtg
     6841 acgaaacagg tgatgtttcg tattattctt ccgccttaag agatcgccgc caaaatagcg
     6901 acagggccca ggtttaggga ggatcggcgt caagcgacat agggggaatg gccctggaga
     6961 gaaggggccg gcgtcttagc gctaagaagc tccggcaaga tcacccgttg atcttgcctt
     7021 attttttgcc ccggccatcc cgaggtctcg cacacccgtc cgctgttttt tcagttgttg
     7081 cgctcgggct tgaattcgaa gatcaggccg gtctcgacct cggccgggtg gccctgcacc
     7141 tcgtacggct tgaagcgcca ttgagcgagc gccgtctcga tcgcggtctt ctgctcggga
     7201 aaggcgctga tgacatggat atgcttcacc gcgccgtcgg tgccgatgac gatgcgaacg
     7261 ggaatcttga gaaagcgcgg accggccggt gccggctcga cccgatggac gatattcgtg
     7321 gcgctcgcat agtcatggat gcagaccggc gctgttgttc cggcatcgcc gccgctatcc
     7381 gcctcgctcg gcaggatcat cttgtcgagg ctgcggaccg cgctctgcag cgcttccgtg
     7441 tcggtggtgg tgaagttgaa gatcacgaga tggcagcgca gctcggtcgc gaaccaggcg
     7501 cgatagagcc cgccggcgct gtagtcgacg cgcatgaagc tgcgacccgc gatcctcgtc
     7561 tctgcaggct cgaggtcgat ggtcatcttg ggcacctggg cttcggcatc gcgcagctcg
     7621 ccggccgcct gttttgcggt gatcatcggc ttcgcgccga agaacatgtc ttgcgcaaca
     7681 acgagcagcg agggctgtcc cagcgacctg ctgtcgagcg aggcgagcac ataatacccg
     7741 gtgtaggacg gcggcggccc cttcaagcct tccgtccagc cgggcggcag cgcataggag
     7801 agaccgaagt agttgttcac ataagcaccg gccgcgaccg acccgtcctc gggatccgga
     7861 atgttcgcca tcgtcgccga cgaggcatcg gcgagcgcga cgctcgcgcg caacagcatg
     7921 agtattccca agacgcacga tatcgcctgc acgcttttct ccgacagggt cacgacgggc
     7981 gaaagctgat cagatccgcc tcggcgacga gatagagcac gaccgacagg actgctgcaa
     8041 tcccggtcgt caccggagcc ttgacgccgg ggcgcgggct ggacgccgct ccgacggcat
     8101 ggctgggatc gccggcgtga cccaaaatgg cagcacggca aagatcacca cccaccaaat
     8161 gacgacccga gcggcgatcg tctaaacccc aggtcgtagc cgccgaaagc aaatagccac
     8221 ttttcctgta agtgcataga cctgcaggaa gatttactgt cggttgcacg cggctatcct
     8281 gtggtttccc cccttctagg gatcgaagtt tttgattgaa ctttacctta aaatcattct
     8341 acaaatcata tcacgattca accatcgatc tcgggcatca atctcagggg tggagggtgc
     8401 gctatgtata aaagctctgg tacgcagcat cttttgttga cgacggtaag cgcgatcgcc
     8461 ttgcttctac cgctggcgac gcacaaggct catgcgcagg cagcagcagg ggtcagcgac
     8521 ccgggggtgc gtggcggtcc tgcgggagcg ggcggagcgc ttcccggcct caacagcgac
     8581 gagttgaatt tcttcttcca gacgcgggcg cgcttccagg aagtcgactc cgtccaaggg
     8641 accatcgccg atgtcgaccc gggcctgccg accccggaga ccggcggcgg cctcggcccc
     8701 cgcttcaacg gcaacgcctg tgccgtatgc catattcagc cggccgtggg cggttcgagc
     8761 ccgacgacga acccgcagat caggttggcg accctcgacg gcgccaccaa caccatcccg
     8821 tcctttatca gcaacaccag cccggtccgc gaggcccgct tcatcacgaa tccgaacggc
     8881 acgcctgacg gcggcgtgca cgacctcttc gtcatcaccg gccgcaccga cgcccagggc
     8941 tgcaatatcc tccagccaaa cttccctcag gccgtggcgg cgaacaacgt catcttccgc
     9001 atccccatca gtgtcaacgg cgatgggctg gtggagaaca cgcaggactc ggcgcttctg
     9061 gcggacgcgc agaccgctcc ggatgggccg gcgagcctgg gcatcaccgt cggcttcttc
     9121 aacaccaacg gcaatgacgg caccgccacc aaattcggct ggaaggcgca gaacaagtcg
     9181 ctgctgctgt tctcgggcga ggcctacaac gtcgagcagg gcgtcaccaa cgagcttttc
     9241 aacaacgagc gcgacgagac gcaaggctgc gtcttcaacc cgacgcccga ggacgcgacc
     9301 gcgctggaga acacgcgcaa cagcaacagc ccggcgtcgg acttctcgtc ggacatcgtc
     9361 aatttcgcag gcttcatgcg cctctcggcg ccgcccgcgc ccgcggcgag cagcaatgcc
     9421 acggtccagg gctcgaacca gttcgtcaac atcggctgcg ccgcctgcca tgtcacgacc
     9481 cacaccacgg cggcgtcgat cttcaccaac cagagcaacg tggcgttcca cccgttcagc
     9541 gacttcgcgc tccacaacat gggaacgggc ctgcaggatc aggtgagcca gggagccgcc
     9601 aacggccagc agttccgctc cgcgccgctc tggggcgtcg gcaagcgcat tttcttcctg
     9661 catgacggcc gtaccaacaa cctcgtcacg gcgatcgagg cgcattcgag ctcgggctcc
     9721 gaagcgaacc aggtgatcgc caacttcaac aagctgtcgg ccagcgatca gcaggacatc
     9781 attcttttcc tgcgctcgct ctgaggtttg gcattgggcg acggcggacg gtttgcccgt
     9841 ccgccgttct ttttattccc atctgctgcc cggtcgcgct catgccggcc gaaagctgac
     9901 gaggtcgctg cgggtgacgg catagagcac gagccatagc accgccgcga tcaccgtcgt
     9961 cacggcggcc ttgatgccga gccgcggatt ggacggtgcg ccgacggcat ggccgagatc
    10021 ccctttctcg accggcctca cgccgaatgg cagaacggcg aaaatcacca cccaccagat
    10081 gatcacataa gcgacgatcg cctgaaccca ggtcatcgca gccgtgtcaa acctgctcca
    10141 gctcgaccag cgtgccgcag aaatccttgg gatgcaggaa cagcacgggc ttgtcatggg
    10201 cgccgatctt gggctcgccg tcgccgacga cccgcgcgcc ttcggccttg agcttgtcgc
    10261 gggcggcgat gatgtcggcg acctcgtagc agacatgatg catgccgccc gagggattgc
    10321 gcgcgagaaa gttgctgacc ggcgagtccg ggccatacgg ctcgagcagc tcgaccttgg
    10381 tgttgtcgag agtgacgaag accacggtca cgccatgggg cggcagcgac acggggtccg
    10441 acaccctggc gccgagcgtc tcgcgataga cggtcgcggc ctttcttacg tccgggacga
    10501 caatggcgac atggttgagc ttgccgatca tgggtgtctc cgtcagatcc gcaccaagtg
    10561 tatttcggtc accggctttt ttccatgcca ggcgcggagc gagcgccgca cggcgagccg
    10621 cgccgcctcg cggagcgccg cgtcgtcgcg ccgctcgcgc ggtccgagct cggcaagggc
    10681 cgcctcgacc ttctgccgca gctcctcggt caacgccgcg tcctgctccg gctcgcccag
    10741 cccctgcacc gtcacctggg gcggcgccag cagccggccc tcgccgtcga cgacgatggt
    10801 ggccagggcc gcgccgttga acgtcatacg ctggcgcgag cgcagcgctt cgctgcccgc
    10861 cggcagcagg cgcgtcccgt ccaccaccag ccgaccggtc gacacctcgc caatgaccgc
    10921 agccggaccc ggtgcaagct tcaccacctc gccattcctc gtaacgatcg tctgcggcac
    10981 ctggcattgc tcggcaagcc gcgcttgcgc cagaagatgg cgggtctcgc catggaccgg
    11041 tacggcgatc ctggggcgca ccatctggta cattcgcgtc agctcgtcct gggcgggatg
    11101 ccccgagaca tggacgaggt gatcgtgctc cgtgatgagc tcgatgccga gaccggcgag
    11161 ccggttctgc aatcggccga tggcgcgctc gttgccgggg atgacgcgcg aggagaagat
    11221 gacggtatca ccctcctcga ggacgatctc gggatggtcg tcctcggcga tgcgggcgag
    11281 cgccgagcgc ggctcgccct ggcttccggt gcagaccagc agcaccttgt cgcgcggcag
    11341 atagcttgct tcctcatcgg tgaggaacgg cggaaggccc tcgagatagc cggtctcgcg
    11401 cgccgcatgc tcgatgcgcc agagcgaccg tccgaccagc gccgcgtggc gatcatgcgc
    11461 ggccgccgcc gcggccgcgg attcgaggcg agcgacattc gaggcgaagc aggcgatgac
    11521 cacgcggttg cggcatttgc cgacgagttc gatcagcgac ttgcgcacat cggcctcgga
    11581 gcccgactct cccggacgca gcgcgttggt cgaatcgcag accatcgcga gcacgcccgc
    11641 ctcgccgagg cggcgcaacg ccgccatgtc gctgagctcg cccaccagcg gatcgcgatc
    11701 gaacttccag tcgccggtgt gcagcaccgt gccgagcggc gtgcgcagca cgacggcgtt
    11761 gggctcgggg atcgaatggg tgagcgtcac cagttcgatc tcgaaagggc cgacggtgaa
    11821 cttgccggac atcggcactt cgatgatcgg cacctgatgc tcgagcccgg cctcgaccag
    11881 cttcagccgc agcaccgcgg cggtgaaagg cgtcgcatag acggggcaac gcaggcgcgg
    11941 ccagagatag ggaatggcgc cgatatggtc ctcatgggcg tgggtcacga tgagacccgc
    12001 caggtccgcg gcgcgctcgg cgatgaatcc gggatccggc atgatcacgt cgacgccggg
    12061 caaggtgtca tcggcaaagg agatgccgca atcgatcatc agccagcgcc cgccatagcc
    12121 atagagcgac agattcatgc cgatctcgcc gacgccgccc agcgccagga agacgagctc
    12181 gtccggccgg ttgggtgtcg tgttgctcat ggtttgcggt tccgctcata gacgaggcgc
    12241 aggccccaga gcgtgaggtt cgggtcgacg cgatcgatcc cggggatcgc gccctcgaac
    12301 agcggcacga ggcctcccgt ggcaatgacg cccatgggcg cgccgaactc tttcttgatg
    12361 cgcgccacca gcccctcgac aaggccgaca tagccccaga acacgcccga ctgcatgcag
    12421 gccaccgtgt ccttgccgat cacgtggtcg atgcggcgta gccgcacgct cggcagcttg
    12481 gcggcggcca tgtgcagagc ggtgatcgag agcgtcacgc ccggcgcgat gacgccgccg
    12541 caataattgc cgtccttgtc gacgacgtcg aaattcgtcg ccgtgccgaa atcgacgacg
    12601 acgagcggcc ccttgtaccg atcatgggcc gcgacggtgt tgagaagacg gtcggcgccg
    12661 acctcctccg gccggtcgac cagcgccttc gtcccgagga cgacccccgg ctccccgacc
    12721 accagcgggt cgctgttgca atagcgccgg cacagcgtga ccagattaaa attggcgtcg
    12781 ggcacgacgc tgccgatgat cgcgcccgtg aggccgttgc gcgacagacc ctcgagcgcc
    12841 agcaggtgat cgagccacac gacatattca tcggcggtgc gcctggcgtc ggtggcggca
    12901 cgccacgcgc ccttgagctg atcgccttcc cacacggaaa acaccgtgtt ggtgttgttg
    12961 gcgttgatgg cgagcagcat cctgcctcct cccccggctc acgcgcccgc cgggaagacg
    13021 tcgccagcgg tgatccggcg ctgggccgtc ccttcttcga gcagcagagc gccctcctcg
    13081 tcgagcccgg cgaagcggcc gcgcgtctcg ctgccctgga gccgcacgcg aatgtcgtcg
    13141 ccgaggccgt cagcgccggc aagccatgcc gcgcgcagcg gcggaaagcc ttcgacccgc
    13201 cagcgctcgt gccaatggta gaaacgcccg gccacagctt ccagcatcgt ctcgggcgtc
    13261 gccgcgtcgg cgcctgcagc gcgaagcgag atcgccgggg tttcgacatt ctcggggtag
    13321 gaggcgaggt tgacgccgat gccgaccacg agccacgcga gccgcccgcc ggcagcgccc
    13381 tgggattcga gcaggatccc gctcgccttg cgtccctcga ccagcacgtc gttgggccat
    13441 ttgtaggcga gcttcgtccc ggccggcagg aacgaccgcg ccgcctcgcc caccgccaac
    13501 gccgcggcga aaccgagctg cgccgcctgc gctgccgcat gatcgggacg cagcaggatc
    13561 gacatgtaga gattgcccgg cggcgagacg aaggaacggc cgcggcggcc gcgtcccgcc
    13621 gtctgggcgc cggcccagac gagcaagccc gccggcgctc cctgcgcggc ttgacgcttc
    13681 gcttcctcgt tggtgctgtc gagctcggcg acgctgagca ggcgaaagaa ggggggaagc
    13741 cgcggcgtcg cggtcatccc ggaaagaggg cgccggcagc ggccgcggca gccccggcga
    13801 tgggtgacag gaagaaggcg aagagcagaa tggcaatcgc cgtgaacgcc agcagcccct
    13861 gcatctcgcc gccgaagggc tggtcgaaag cggccgtcgg ctcgtcgaaa tacatgatct
    13921 tgacgatgcg gagatagtag taggcggcca ccacgctggt gacgacgcca atgactgaaa
    13981 gcgaatagag gccggaattg atggcagaca tgaagatata gaatttggcg aagaacccgc
    14041 ccagaggcgg gatgccggcc atcgagaaca tgaagatcgc gagcgctgcc gcgaggccgg
    14101 gatgggtgcg cgagaggccg gccaggtcgt cgatgccttc gaccgcccgc ccctgacggc
    14161 gcatgcagag gatgacggcg aaggtcccga cattcatgaa gagatagatg gcgagataga
    14221 tgagaatgcc gcgcacgccg gtctcgctgg tcgcggcaag gccgatcagc atgtagccga
    14281 catggccgat cgagctatag gccatcagcc gcttgatgtt gcgctggttg atcgccgcga
    14341 acgcaccgag cagcatcgag ccgacggcga tgacccagat gatctggcgc cactgatcgt
    14401 cgagatggcc gaagggatag gtcatgacgc ggatgaagag cgccatggcg gcgatcttgg
    14461 gcgctaccga gaagaaagcc gtcgccggcg tcggcgcgcc ttcgtagacg tcgggcgtcc
    14521 acatatggaa gggcacggcc gacaccttga aggcgaggcc gacggcgatg aagacgatgc
    14581 cgatgacgag gccgatcgag tagccgtcgc tcgtatccaa gctgtgcgcc agcgtgtcga
    14641 aattggtgct gccggcgaag ccgtagacca tcgacgcgcc gtagagcagc atgcccgagg
    14701 agagcgcgcc caggacgaaa tatttgagcc cggcctcggt cgagcgctcg ctgtcgcgct
    14761 ggaagctggc gatcacgtaa agcgcgagat tctgtagctc gaggccgaga tagagcgaga
    14821 tcaggtcgtt ggccgagatc atgaccatca tgccgacggt cgcgagcagc accagcaccg
    14881 ggtactcgaa gcgctcgatc tgctccctct cgttgaagcg catcgacagg atgatggaga
    14941 gcagcgaccc gatcagcacc agaatcttca tgaaaacggc gaagccgtcg gtgaagaaca
    15001 tgccatagaa agtgacctgt cgctcgaacc cggaagcggc gacgagcacg gcggtgatgg
    15061 cgagcacgag gacggaaagc caggtgatct cgcgcgtcgc ataggcggcg tgctggaacg
    15121 cgccgagcag cagcagcgcc atggcgctcg ccgcgaggaa gatctcgggc agcgcgggga
    15181 gaaagctggg aagagcgtcg gcggtcatgg gccggcctcc gccagcttgg ccgcggcctc
    15241 gtcgcgaacc gcggcctgga cgcgctcgac gaggttggcg acggaaggct ggatcggccg
    15301 caggaaggag ctgggataga cgcccatcca cagcacgatg gcgatcatcg gcgcgaagat
    15361 ggcgacttcg cgccagttga gatcgagcat ggcgcggaca tcctcgcgcg tgatcgtgcc
    15421 gaagatgatg cggcgataga gatagagcat gtaggtagcg cccaggatga tgccggtggt
    15481 ggcaaagaac gtgacggccg tgttcacctg gaaggtgccg atgagcgaca gaaactcgcc
    15541 gacgaaaccg ctggttccgg gcaagcccac ggatgccagc atgaacacca tgaacaccag
    15601 ggcatatttc ggcatgttgt cggccagccc gccatagcgc gcgatctcgc gcgtgtgaag
    15661 gcggtcatag acgacgccga cgcagaggaa gagcgcggcc gagacgatgc catggctcag
    15721 catctgcacc atcgcgccct ggaggccctc ggacgtcatg gcgaaggtac ccatggtgac
    15781 gaagcccata tgcgccaccg aggaataggc gatgagcttc ttcatgtcct cctgcgccag
    15841 cgccaccagc gaggtgtaga tgatggcgac gacgctcagc acgaagaccc aggtggcgag
    15901 atcgtgcgac gccagcggca gcatcggcag cgagaagcgg atgaagccgt aaccccccat
    15961 cttgaggagc acgcccgcca ggatgacgga gcccgccgtc ggcgcctcga catgggcgtc
    16021 gggcagccaa gtatggaccg gccacatcgg caccttgacg gcgaaggagg cgaagaaggc
    16081 gagccacagc catgtctgca cccgcggcgc gtccttgagg atgacgggca agcggtcgag
    16141 cgcctcgacg atgtcggtcg agccgacata cgaatagatc gccaggatgg cgagcagcat
    16201 cagcacagaa cccaggagcg tgaagaggaa gaatttgaaa gcggcataga cgcgccgcgg
    16261 gccgccccag acgccgatga tgaggaacat cgggatcagc acgccctcga agaagacgta
    16321 gaagatgacg aagtcgaggg cggagaacat gccgaccatc atcgtctcga ggacgaggaa
    16381 cgcgatcatg tattccttga cgcgccgcgt gaccgaggtc cagctggcga cgacgcagac
    16441 cggcgtgagg aacgtcgaca gcagcacgaa gaacagcgag atgccgtcga tgcccaagtg
    16501 gtaggcgata ttgaagtcgg gcaaccagtc gacgtgctcg acgaactgga acttgaagct
    16561 cgcgggatcg aaatagcccc agagcaggag cgacagggcg aagtcgatga gcgacgtcca
    16621 gagcgcgatc cagcgcgcgt tgcgcgccac cgcctcgctc tcgccgcgca ggacgacgat
    16681 gaggaggacg ccggccagcg gcaggaaggt gacgagcgag aggagaggcc aatgttccat
    16741 cgcgctcacc agaaggcccg gcgcaggatc gaataggaga cgaaggccac cacgccgatg
    16801 acgatgacga aggcgtattg gtagaggtac ccggtctgca accggctcgc gcgcaccgcg
    16861 agccggacgc tgacggcggc gagcccgtcg ggaccgaagc gatcgatggt gcgcccgtcc
    16921 ccgctctgcc acaggaagcg gccgagccgc atcgccgggc gcacgagcag gaaatcgtag
    16981 agctcgtcga aataccattt gttgagaagg aacaggtaga ccgggcgcaa acgtcgcgcg
    17041 gccagcgccg gcagctcagg ccgccagata taggcgaccc aggcgacggc gatgccgaag
    17101 aggccgacga tcaccgggag cgtcccgacc cagaagggtg cgtgatgcgc cgcctcgagg
    17161 ctcgcatgct ccggcagcac cacgatggaa tcaccccaga aagcctcgcg cccctcgccg
    17221 acgaagaagt tgtagccgag cacgccggcg aaaacggcac cggcggcgag gacatagagc
    17281 ggcgccagca tgacctgcgg cgattcatgg acgtgacgca tgacatcctg gctcgcgcgc
    17341 ggcgcgccgt ggaaggtgag gaagatgagg cgccaggagt agaaggccgt gagaaacgcc
    17401 gccaggactc cgagccagaa gccgtaaagc gcgacgccgg agcccgcgcc ccacgcggcc
    17461 tcgaggatga cgtccttcga gtagaagccg gcaaagccga agacgccggg aatgccgacg
    17521 cccgcgagcg ccaggctgcc gatccacatc agggcgtaag tgaccgggat cagccgccag
    17581 agaccgccca tcttgcgcat gtcctgctcg cccgacatgg cgtggatgac cgagcccgag
    17641 ccgaggaaca gcaatgcctt gaagaaggca tgggtcatca ggtggaagat cgctgccgaa
    17701 taggcggaga cgccggcggc gatgaacatg tagccgagct ggctgcaggt cgaataggcg
    17761 atgacgcgct tgatgtcgtt ctgcgccacg ccgacgcttg ccgcgaagat cgccgtggtg
    17821 gcaccgatga agcagaccac ggcgagcgcg accggtgcat attcgaaaag cggcgaaagt
    17881 cgcgccacca tgaatacgcc ggcggtcacc atggtcgccg catggatcaa ggcagaaacg
    17941 ggtgtcggtc cctccatcgc atcggggagc cacgtgtgga gcccgatctg cgccgatttg
    18001 cccatggcgc cgacgaagag aagaatgcag gtcacggtca gggcatcgac cttgaagccc
    18061 aggaaattga aaggcgcatg ggcaaagttt ggcgccgcgt cgaagaccgt gccgaaatgc
    18121 agcgagccga agagcgcgta aacggcgaag atgcccagca aaaagccgaa atcgccgatg
    18181 cggttgacga cgaaggcctt gatcgccgcg gcattcgccg aggggcggtc ataccagaag
    18241 ccgatcagga gatacgagga gaggcccacg ccctcccagc cgaagaacag ctgcaggaag
    18301 ttgtccgccg tcaccagcat cagcatgaag aaggtgaaga ggctgagata cgccatgaag
    18361 cgcgggatcg aatgatcatg cgccatgtat ccgacggaat agatatgcac catggccgag
    18421 accacggtaa cgaccgcgac catcaccgcg ctcaggctat cgaagcgcag cgcccattgc
    18481 gccttgagat cgcccgtgtc gatccacgtg aggaggtcga cggtcgtgcg cgcctgaccg
    18541 cccaccgaca cctcgacgaa gagaatgacg ccgcagatcg cggcgagcac caggctcgtg
    18601 caggtgacga gctgcgcacc ccgatcgccg atccagcgcc cgaataaccc ggcgacgaac
    18661 gcgcccaaga gcggcaggaa gacggcagcg atggtcatga tggcgcccta tcgccggacg
    18721 acagcgagcg acccccctcc cctccctccc cctcaagggg ggagggggaa gacgaggaag
    18781 ctccgtaaga ccccctcccc ccttgagggg gagggttggg gtggggggta tgtgcagctt
    18841 cgctcatacg cctcagccct tcatcaggtg gatgtcttgg acggcgatgg tgccgcggtt
    18901 gcggaagtag acgacgagga tggcgaggcc gatcgccgcc tcggcggccg ccacggtgag
    18961 cacgaacatg gcgaagatct ggccgaccag atcgtggaga taggccgaga aggcgacgaa
    19021 gttgatgttg acggcgagca gcatcagctc gatcgacatc agcatgatga tgacgttctt
    19081 gcgattgagg aagatgccga agatgccgag cgtgaagatg atcgccgcga ggaccaggta
    19141 atgcgtgagg ccgatctcga gcatcgctaa atcccctggc gcggctggac cttgacgatg
    19201 gcgagcgtct cctggcgccg gcgcgccagt tgcaccgcaa tcttctgccg gcgcacccct
    19261 tcacgggcac gcagcgtcag cacgatcgcg cccaccatgg cgacgagcag caccatgcct
    19321 gcggtctgga aggcatagac atagcgggtg tagatcagcg cgcccaatgc ctgggtgttg
    19381 gtgaggccgg agagcgcggg cgtcggcgcg gcgatgctct tcgccacgtc gggcgccacc
    19441 gtccaggcgg caaaggccag gaacagctcg aggaacagca tcaatccgat gagcgcgccg
    19501 atcggcagat acgtgatgaa cccgcgccgg agctcggcga agtcgatgtc gagcatcatc
    19561 accacgaaga ggaacagcac ggcgacagcg ccgacataga cgatgaccag gatcaaggcg
    19621 aggaactcgg cgccgagcag caggaagaga gcggccgcat tgaagaacgc caaaatcaag
    19681 aacagcacgg aatg
//
LOCUS       DASYNS010000104         8930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2676891, whole genome shotgun sequence.
ACCESSION   DASYNS010000104 DASYNS010000000
VERSION     DASYNS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8930
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2676891"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            959..1837
                     /locus_tag="VGP50_03345"
     CDS             959..1837
                     /locus_tag="VGP50_03345"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV8026437.1"
                     /translation="MVAAAAASGNSFQVLILKLQSYWASKGCVILQPYDVEMGAGTFH
                     PATTLRALGPKAWRAAYVQPSRRPADGRYGENPMRLQHYYQFQVILKPAPAESQQLYL
                     DSLVMLGIDAKHHDIRFLEDDWESPTLGAWGLGWEVQLDGMEVSQFTYFQQVGGIECD
                     PVSTELTYGLERLATYVQGVESVYDLDYNGAGVTYGDVFKRAEYEFSAYNFEHADAEM
                     LLRHFVDAEKECQALLARDLPLPAYDQCVKASHIFNLLDARGVVSVTERAAYIGRVRA
                     LAKGCCEGWLAHQAAA"
     gene            1900..3975
                     /gene="glyS"
                     /locus_tag="VGP50_03350"
     CDS             1900..3975
                     /gene="glyS"
                     /locus_tag="VGP50_03350"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV8026438.1"
                     /translation="MAELLLELLTEEIPARMQARAAEDLAMLATDKLEAAGLAFSDAH
                     ADATPRRLVLVVEGLPTAQADTSEERRGPRVGAPASAVEGFLKSAGLTALAQCEKRDT
                     GKGEFYFAVIRRSGRATAEVLPDILRDVILAVPWPKSMRFPAARFRWVRPMTSALCLF
                     DGKVLPLDLDGVPVGKVTQGHRFLSSGTFEVHAFADYGAKLRRAHVMLRRDDRRQAIA
                     RALAEKAAEAQLIVKDDPALLEEVTGLVEWPVVLMGAIDPAFLDLPPEVLTTSMRANQ
                     KYFACLDRGGKLAARFLVVANMIADDGGSAIIAGNERVLRARLADARFFWEQDLKVKL
                     AARLPKLAERVFHAKLGSVLDKVGRVERLADGLAAHLRDADPKKVRRAAELAKADLST
                     SMVGEFPELQGIVGRYYALASGEAAEIADAIAEHYSPLGPGDRCPSAPVSVAVALADK
                     IDTLVGFFSIGEKPTGSKDPFALRRSALGVIRIILENRLRLKLRRIFALAFGIGTEGE
                     TWAKGSDPGAELLDFFADRLKAHLREQGVRHDLVAAVFALGGEDDLVRLLARVAALDA
                     FLRSDDGANLLTAYRRAANIVRIEEKKDGASYAEPPDPKLLAEPAEATLAAELESAAG
                     RSEAALEREDFAEAMAALAALRGPVDEFFARVTVNCEDRSLRANRLRLLTQIRDTLNR
                     VADFSQIEG"
     gene            3983..6664
                     /gene="ppdK"
                     /locus_tag="VGP50_03355"
     CDS             3983..6664
                     /gene="ppdK"
                     /locus_tag="VGP50_03355"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEV8026439.1"
                     /translation="MAKWVYGFGQGSGEGRAGMKNLLGGKGANLAEMSNLGLPVPPGF
                     TITTEVCTYFYANDKKYPSDLKEQVAAGLAKIEREIGAKLGDAQNPLMVSVRSGARAS
                     MPGMMDTVLNLGLNDQTIEGLAKRSGDPRFAYDSYRRFIQMYGQVVLGVDHHHFEDLL
                     ENTKLDLGVTLDTELKAADWMQVVTGFKEVVQRELGKPFPQDPQEQLWGAIGAVFGSW
                     MNQRAITYRRLHDIPADWGTAVNVQAMVFGNMGEDCATGVAFTRNPSTGANEFYGEYL
                     VNAQGEDVVAGIRTPQQVTIAGKKANNSPLPAMEEVMPAVFAELLKVRGALEVHYRDM
                     QDIEFTVQQGKLFMLQTRTGKRTAPAALKIAVDMANAGVIRREEAVARIEAESLDQLL
                     HPTLDPKAQRTILARGLPASPGAASGIVVFSAGEAEKRAAKGEAVILVRIETSPEDIH
                     GMHAAKGILTARGGMTSHAAVVARGMGRACVAGAGELRIDYRTQTISVRGEVVKAGDV
                     VTINGSTGEVMLGEVPTIQPEMSGDFATLMQWADKMRRMGVRANADTPQDAETARKFG
                     AEGIGLCRTEHMFFNENRIIAVREMIMADDTEGRRKALAKLLPMQRQDFIEIFRIMRG
                     LPVTIRLLDPPLHEFLPKTEKEMQELVGTTGKDIATIRHRADSLHEANPMLGHRGCRL
                     AITGPEIYDMQARAIFEAAAAVVKETGDKVEPEIMIPLIATKKELDILKERIDRIGGE
                     VMKATGVKLDYLVGTMIELPRAVLRAAEIAETAEFFSFGTNDLTQTCLGLSRDDAGSF
                     LGEYLKQGIFEHDPFVTIDRDGVGELVRIGAERGRRARTDLKLGICGEHGGDPASIRF
                     CQEVGLDYVSCSPYRVPIARLAAAQAALAEQAIETTA"
     gene            6750..7682
                     /gene="meaB"
                     /locus_tag="VGP50_03360"
     CDS             6750..7682
                     /gene="meaB"
                     /locus_tag="VGP50_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEV8026440.1"
                     /translation="MTASPLTALARGGKPALAAALARLEQAPEAEATLALLDAAYGSP
                     RAHVVGVTGPPGVGKSTLIGALIGAWRRAGETVGVVAVDPSSRVSGGALLGDRTRLRT
                     DPEDEGVFVRSMAARDKLGGLAALTVSAMVLMRALYDKVIIETVGVGQSETDVAGTAD
                     TVVLCLQPGSGDSLQFMKAGIVEIPHIAVVTKSDLGEPAERARNDALGVLGLAEHDAD
                     DWQVPVLLLSSLRRDGIDQLMEAVARHWRWLGDSDRLASARRAQAAHWLIEGVREAFG
                     RDGLRRVGALTLPGGASPFRAAADLAMKLRGAQS"
     gene            7695..8426
                     /locus_tag="VGP50_03365"
     CDS             7695..8426
                     /locus_tag="VGP50_03365"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010516705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEV8026441.1"
                     /translation="MEELSGRTALVTGGSRGIGRAIALALAQAGADVAVNYRANAAEA
                     ERVCGTIAKLGRRTVKIAADVSAAGDVARLVTATEAALGPVDILVNNAGIAPPVGIEA
                     LTEALWDETIAVNLKSVFLVTQAVLPGMRERRWGRIINISSTAAQIGGIVGPHYAASK
                     AGIHGLTHGYASRLAKEGITANVIAPALIETDMVRALPAAAVDRIPVGRFGRPEEVAE
                     IAVMLARNGYVTGQTINVNGGVYLS"
     gene            8439..8843
                     /locus_tag="VGP50_03370"
     CDS             8439..8843
                     /locus_tag="VGP50_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026442.1"
                     /translation="MGVIFVARSAKLCKWAFDVGLSKHVFKVGYSDDDPKPLVEKGWA
                     GETDWSLVKKRDAEGVDEDEILARLARKEKMVDPTYYPRLRGAKGVFKVPPEHVENHI
                     LVSRAMAGDNARIDLKLKPADFADYLIHNALK"
BASE COUNT         1507 a         2994 c         3004 g         1425 t
ORIGIN      
        1 gccccctcac cccggccctc tccccggagg ccccggaggg gagaggggga tgtgtggcgc
       61 ggtcaaagca acgctgcttt agacagaacg acgacccatc ccaaattgtc actgaagcgg
      121 cgctagggtc ggcagggaag caacggcaca gcatgaaata ccttttccgc accaagagtg
      181 cggtgtatcg cgcggccttc tggggcggct tttgggaggg tgcgagcgcg gcgagcctga
      241 cctacacacg ctcgcgttgc tatgagcacg ttggaaactt gaaaacggct tggacgaggg
      301 tcggagacta tatctaccaa ggcatcgccc aagtagatag cgaagtacag aataaagcta
      361 agggcgctga ccaggcgcgc ccctcgaaag cggaacgcgt cgcctagagc gctttccgtt
      421 caagtcgacc cattttgcat aggcgttaac gtaggctcct ttagatctta gaatttccgc
      481 catcgaaggg attggagatc ggggaggtgg caggggattg gggggagagc ggtgagggcg
      541 tcgacgatgc gcttacgcga gcgatcatcg atgcgatcat caaagttcac aaggtccttg
      601 gccctggttt cggcgaagca atctatcgcc gggctctcgt cgtcgaattg aaggtcaata
      661 tcgcttggac ctgcttatcg aaggccctgt catcgttgag gtgaaggtgg tcgaagccct
      721 cagcaaggtc cactattcac aagttcgctc atatcttcga gcgacaggcc tgtctgttgc
      781 tctgttggtg aacttcgacc atcccaaggc cgattatcga cgggtcgtct cccctccatc
      841 cccaatcatc cccccgatcg cctgatcttt tgatacggcc gcgcggcgcg ttgccttgac
      901 cctccgcggg cacggcctct atggtgcggc gcaacttaaa cctttgccgg acagccggat
      961 ggtggccgcg gccgcagcga gcgggaacag ctttcaggtc ctgatcctca agctgcagag
     1021 ctattgggcg agcaaaggct gcgtcatcct ccagccctat gacgtcgaga tgggagcggg
     1081 aacctttcac cccgcgacta ccctgcgcgc gcttgggcct aaggcgtggc gcgccgcgta
     1141 tgtccagcct tcgcgccgac cggccgatgg ccgctacggc gagaacccga tgcggctcca
     1201 gcactactac cagttccagg tgatcctgaa gccggcgccg gccgagagcc agcagcttta
     1261 tctcgacagc ctcgtcatgc tcggcatcga cgcaaagcac cacgacatcc gcttcctcga
     1321 ggatgattgg gaaagcccga cactcggcgc ctggggcctc ggctgggaag tgcagctcga
     1381 cggcatggaa gtgtcgcaat tcacctattt ccagcaggtg ggcggcatcg aatgcgatcc
     1441 ggtgtcgacc gagctcacct atggcctgga gcgcctcgcc acgtatgtgc agggggtcga
     1501 gagcgtctat gatctcgact acaacggcgc cggcgtaacg tatggcgacg tcttcaaacg
     1561 cgcggaatac gagttctccg cctataattt cgagcatgcc gatgcggaga tgctgttgcg
     1621 ccacttcgtc gatgccgaga aggagtgcca ggcgctgctg gcgcgcgacc tgccgctccc
     1681 cgcttacgac caatgcgtca aggcgagcca catcttcaat ctgcttgacg cgcggggcgt
     1741 cgtctcggtg accgagcgcg ccgcctatat cggccgcgtg cgcgcgctgg ccaagggctg
     1801 ctgcgagggc tggctcgcgc atcaggcggc cgcctagagc atgttccagt caaccagaac
     1861 catttttgcc gtcgatttga acgggatctg atccagacga tggccgagct tctcctcgag
     1921 ctcttgaccg aagagatccc ggcgcgcatg caggcgcgcg cggccgagga tctcgccatg
     1981 ctggcgaccg acaagctcga agcggcgggc ctcgccttct ccgacgccca tgccgacgcg
     2041 acgccgcggc ggctcgtgct ggtggtggag ggactgccga cggcgcaggc cgatacgagc
     2101 gaggagcgcc gcgggccgcg cgtcggcgcg ccggcgagcg cggtcgaggg tttcctcaaa
     2161 tccgccggtc tcaccgcgct cgctcaatgc gagaagcgcg acacgggcaa aggcgaattc
     2221 tatttcgccg tgatccggcg cagcgggcgc gcgacggccg aggttctccc cgacatcttg
     2281 cgcgacgtga ttctcgcggt gccctggccc aaatcgatgc gctttcccgc ggcgcgcttc
     2341 cgctgggtcc ggccgatgac cagcgcgctc tgcctgttcg acggcaaggt cctgccgctc
     2401 gatctcgacg gcgtgcccgt gggaaaggtg acgcaaggcc atcgctttct cagctccggt
     2461 acattcgagg tgcacgcttt cgccgattac ggcgcgaagc tgcgccgggc gcatgtcatg
     2521 ctgcggcgcg acgaccggcg ccaggcgatc gcgcgggcgc tcgccgagaa ggccgccgaa
     2581 gcgcagctca tcgtcaagga cgacccggcg ctgctcgagg aagtgaccgg cctcgtcgaa
     2641 tggccggtgg tgctgatggg ggcgatcgat ccggcgtttc tcgacttgcc gcccgaagtg
     2701 ctgacgacct cgatgcgtgc caatcagaaa tacttcgcct gcctcgaccg cggcggcaag
     2761 ctcgcggcgc gcttcctcgt ggtcgccaac atgatcgccg atgacggcgg gagcgcgatc
     2821 atcgccggca atgagcgcgt gctgcgggcg cgactggccg atgcgcggtt cttctgggag
     2881 caggatctga aggtgaagct ggcggcccgc ctgccgaaac tcgccgagcg cgtgttccat
     2941 gcgaagcttg gcagcgtgct cgacaaggtc ggccgggtcg agcgtctcgc cgacggtctg
     3001 gcggctcatc tgcgcgatgc cgacccgaag aaggtgcgcc gcgccgccga gctcgccaag
     3061 gccgatctgt cgaccagcat ggtcggggag tttcccgagc tgcaggggat cgtggggcgc
     3121 tactatgcgc tggcgagcgg cgaggccgcg gagattgccg acgccatcgc cgagcattat
     3181 tccccgctcg ggccgggcga ccgttgcccg agcgcgccgg tcagcgtcgc ggtggcgctc
     3241 gccgacaaga tcgacacgct ggtcgggttt ttctcgatcg gcgagaagcc gacgggctcg
     3301 aaggatccct tcgcgttgcg ccgctcggcg ctgggcgtca tccgcatcat cctcgagaac
     3361 cggctgcgcc tgaagctgcg gcgcatcttc gctctcgcct ttggcatcgg caccgagggc
     3421 gagacctggg caaagggcag cgatcccggc gccgagcttc tcgacttctt cgccgaccgc
     3481 ctcaaggcgc atctgcgcga gcagggggtg cgccacgacc tcgtcgccgc cgtcttcgcg
     3541 ctcggcggcg aggacgatct cgtccgtctc ctcgcccgcg tcgcggcgct cgacgcgttc
     3601 ctgcgcagcg acgacggcgc caacctcctc acggcctacc gccgcgccgc caacatcgtc
     3661 cgcatcgagg aaaagaagga cggcgcctcc tatgccgagc cgcccgaccc gaagctgctg
     3721 gcggagcccg ccgaagcaac cctcgccgcc gagctcgaga gcgcggccgg gcggagcgaa
     3781 gccgccctcg agcgcgagga ctttgccgag gcgatggcgg cgttggccgc gctgcgcggc
     3841 ccggtcgatg aattctttgc gcgcgtaacc gttaattgcg aggacaggag cctcagggcc
     3901 aatcgcctgc ggctcctgac gcaaatccgc gacacgctca accgcgttgc ggatttttcg
     3961 caaatcgaag ggtaggtggg cgatggcgaa atgggtctat ggcttcggtc agggcagcgg
     4021 cgagggccgc gccgggatga agaacctgct gggcggcaag ggcgccaatc tcgcggagat
     4081 gagcaatctc ggcctgccgg tgccgccggg cttcaccatc accacggagg tctgcaccta
     4141 tttctacgcc aacgacaaga aatacccttc cgacctgaag gagcaggtcg cggcgggcct
     4201 ggccaagatc gagcgcgaga tcggcgccaa gctcggcgat gcgcagaatc cgctcatggt
     4261 ctcggtgcgc tcgggcgcgc gcgcctcgat gccgggcatg atggacacgg tgctcaatct
     4321 cggcctcaac gaccagacca tcgagggcct ggcgaagcgc agcggcgatc cgcgcttcgc
     4381 ctatgacagc tatcgccgct tcatccagat gtacgggcag gtggtgctcg gcgtcgatca
     4441 tcatcacttc gaggatctgc tggagaacac caagctcgat ctcggcgtca cgctcgacac
     4501 cgagctcaag gcggcggatt ggatgcaggt cgtcaccggc ttcaaggagg tcgtgcagcg
     4561 cgagctgggg aagccctttc cccaggaccc gcaggagcag ctttggggcg ccatcggcgc
     4621 cgtcttcggc tcctggatga accagcgcgc catcacctat cgccggctgc acgacattcc
     4681 ggccgattgg ggcaccgcgg tcaatgtcca ggccatggtc ttcggcaata tgggcgagga
     4741 ttgcgcgacc ggcgtcgcct tcacccgcaa tccctcgacc ggcgccaacg agttctatgg
     4801 cgagtatctc gtcaacgccc agggcgagga cgtggtggcc ggcatccgca cgccgcagca
     4861 ggtgacgatc gccggcaaga aggcgaacaa ctcgccgctt ccggccatgg aagaagtgat
     4921 gcccgccgtc ttcgccgagc tcctgaaggt gcggggcgcg ctcgaagtcc attaccgcga
     4981 catgcaggac atcgagttca cggtgcagca gggcaagctc ttcatgctgc agacgcgcac
     5041 cggcaagcgc accgcgccgg ccgcgctcaa gatcgccgtc gacatggcca atgccggggt
     5101 catccgccgc gaggaggcgg tggcccgcat cgaggccgag tcgctggatc agctgctcca
     5161 tccgacgctc gacccgaagg cgcagcgcac cattctggcg cgcggcctgc cggcatcgcc
     5221 cggcgccgct tcgggcatcg tcgtattttc cgccggcgag gcggagaagc gcgcggccaa
     5281 gggcgaagcc gtgatcctcg tgcgcatcga gacgagcccc gaggacatcc atggcatgca
     5341 cgccgccaaa ggcatcctca cggcgcgcgg cggcatgacc agccacgcgg ccgtcgtggc
     5401 gcgcggcatg ggccgcgcct gtgtcgcggg cgcgggcgag ctgcgcatcg actaccgcac
     5461 gcagaccatc agcgtgcgtg gcgaggtcgt gaaagcgggc gacgtcgtca ccatcaacgg
     5521 ctcgaccggc gaggtcatgc tgggcgaggt gccgaccatc cagccggaga tgtcgggcga
     5581 tttcgcgacg ctcatgcaat gggccgacaa aatgcggcgc atgggcgtgc gcgccaatgc
     5641 cgacacgccc caggacgcgg agaccgcgcg caaattcggc gccgaaggca tcggcctctg
     5701 ccgcaccgag cacatgttct tcaacgagaa ccgcatcatc gccgtgcgcg agatgatcat
     5761 ggcggacgat accgagggac ggcgcaaggc gctcgccaag ctcctgccga tgcagcgcca
     5821 ggatttcatc gagatcttcc gcatcatgcg cggactgccg gtgacgatcc gcttgctcga
     5881 cccgccgctc cacgaattct tgcccaagac cgagaaggag atgcaggagc tggtgggcac
     5941 gaccggcaag gacatcgcca ccatccgcca tcgcgccgat tcgctccacg aggccaatcc
     6001 gatgctcggt catcgcggct gccgcctcgc catcaccgga cccgagatct atgacatgca
     6061 ggcgcgcgcc atcttcgagg cggccgccgc cgtcgtgaaa gagacgggcg acaaggtcga
     6121 gcccgagatc atgatcccgc tcatcgccac caagaaggag ctcgacattc tcaaggaacg
     6181 gatcgaccgc atcggcggcg aagtgatgaa agcgacgggc gtcaagctcg actatctcgt
     6241 cggcacgatg atcgagctgc cgcgcgccgt gctgcgcgcc gccgagatcg ccgagaccgc
     6301 cgagttcttc agcttcggca ccaacgacct gacgcagacc tgccttggcc tgtcgcgcga
     6361 cgacgccggg agcttcctcg gcgagtatct gaagcagggc attttcgagc acgatccctt
     6421 cgtcaccatc gaccgcgacg gcgtcggcga gctggtgcgg atcggcgccg agcgcggccg
     6481 gcgcgcgcgc acggacttga agctcggcat ctgcggcgag catggcggcg atcccgcctc
     6541 catccgcttc tgccaggagg tcggcctcga ctacgtctcg tgctcgccct atcgcgtccc
     6601 catcgcccgc ctcgccgccg cgcaagcggc gctcgccgag caggcgatcg agacgacggc
     6661 gtgaccgggg cgcgtcaccc ctcaccccaa ccctctcccg caaggggaga gggagttcac
     6721 cgagggcgtg tcgaatatcc cccgacccca tgaccgcgtc gcccctcacc gcgctcgcgc
     6781 gcggcgggaa gcccgcgctc gccgccgccc tcgcgcgcct cgagcaggcg cccgaggccg
     6841 aggcgacgct ggcgctgctc gatgccgcct atggcagtcc gcgcgctcat gtcgtcggcg
     6901 tcaccggtcc gccgggcgtc ggcaagtcga cgctgatcgg cgcgctcatc ggcgcgtggc
     6961 ggcgcgcggg cgagaccgtt ggcgtcgtcg ccgtcgaccc gtcctcgcgc gtctcgggcg
     7021 gcgcgctcct gggcgaccgc acgcggctcc gcacggatcc cgaggatgaa ggcgtcttcg
     7081 tgcgctcgat ggcggcgcgc gacaagctgg gcggcctcgc cgcgctcacc gtgtcggcga
     7141 tggtcctgat gcgcgcgctc tacgacaagg tcatcatcga gaccgtcggc gtcgggcaat
     7201 ccgagaccga cgtcgcgggc accgccgata cggtggtgct gtgccttcag cccggctccg
     7261 gcgacagcct gcaattcatg aaggccggca tcgtcgagat cccgcatatc gccgtcgtga
     7321 ccaaaagcga cctgggcgag cccgccgagc gcgcgcgcaa cgatgcgctg ggggtgctcg
     7381 gcctggccga gcatgacgcc gacgactggc aggtcccggt gctcctgctc tcgtcgctgc
     7441 gccgcgacgg catcgaccag ctcatggagg cggtcgcccg gcattggcgt tggctgggcg
     7501 atagcgaccg cctcgcgtcc gcgcgccgcg cgcaggcggc gcattggctg atcgagggcg
     7561 tgcgcgaggc cttcggccgc gacggcttgc ggcgggtggg cgcgctcacc ctgccgggcg
     7621 gtgcctcgcc ctttcgcgcc gccgccgatc tcgccatgaa gctgcgcggc gctcagtcat
     7681 agcggagacg ggacgtggaa gagctctcgg gtcggacggc cttggtcacg ggcggcagcc
     7741 gcggcatcgg ccgcgccatc gcgctggcgc tggcccaagc gggcgcggat gttgccgtga
     7801 actaccgcgc gaacgctgcc gaggccgagc gggtctgcgg cacgatcgcc aagctcggaa
     7861 ggcgcaccgt gaagatcgcc gccgacgtgt cggcggcggg cgacgtcgcg cggctcgtca
     7921 ccgcgaccga ggcggcgctg ggtcccgtcg acatcctcgt caacaatgcc ggcatcgcgc
     7981 cgccggtcgg catcgaggcg ctgaccgagg cgctctggga cgagaccatc gccgtcaacc
     8041 tcaaatcggt cttcctcgtg acgcaagcgg tgctgccggg catgcgcgag cggcgctggg
     8101 gccgcatcat caacatctcc tcgacggcgg cgcagatcgg cggcatcgtc gggccgcatt
     8161 acgccgcgtc gaaagccggc attcatggct tgacccacgg ctatgcgtcg cggctcgcca
     8221 aggagggcat caccgccaac gtcatcgccc cggcgctcat cgagacggac atggtgcggg
     8281 cgctccctgc cgcggcagtg gatcgcatcc cggtcggccg tttcgggcgg cccgaagagg
     8341 tcgcggagat cgcggtgatg ctcgcgcgca acggctatgt cacggggcag acgatcaacg
     8401 tcaatggcgg cgtctatcta agctgacggg gagggacgat gggggtgatc ttcgtggcgc
     8461 ggagcgcgaa gctttgcaaa tgggcgttcg atgtcggcct cagcaagcat gtcttcaagg
     8521 tcggctacag cgatgacgat ccgaagccgc tggtcgagaa gggctgggcc ggcgagaccg
     8581 actggtcgct cgtcaagaaa cgcgacgccg agggtgtcga cgaggacgag atcctggcgc
     8641 gcctggcgcg caaggagaag atggtcgacc cgacctatta cccgcggctg cgcggcgcca
     8701 agggcgtctt caaggtcccg cccgagcatg tcgagaacca tattctcgtg agccgcgcca
     8761 tggccggcga taacgcgcgc atcgatctga agctgaagcc cgccgacttc gccgactacc
     8821 tcatccacaa cgcgctgaag taacaccctc cgcgagagcg ctatcccatc cacacctgcg
     8881 gtatgactca ctctccgccc ctcaggggcg gagagggagt acggtcgtcg
//
LOCUS       DASYNS010000105         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5017463, whole genome shotgun sequence.
ACCESSION   DASYNS010000105 DASYNS010000000
VERSION     DASYNS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5017463"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /gene="scpA"
                     /locus_tag="VGP50_03375"
     CDS             <1..1545
                     /gene="scpA"
                     /locus_tag="VGP50_03375"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEV8026443.1"
                     /translation="IYPPAPSMRIVADIIEYTAKHMPKFNSISISGYHMQEAGATAVQ
                     ELAFTLADGLDYVRAALSKGLKIDDFAPRLSFFFCIGMNFFMEIAKLRAARYLWAKLI
                     APFHPANPASLALRTHCQTSGVSLTAQDPYNNVVRTTIEALAAVLGGTQSLHTNSFDE
                     ALALPTPQSARIARNTQLILAEETGITHVVDPLGGSYYVESLTHSLVAAAEALIREVE
                     SLGGMTKAVESGMPKLRIEEAAARRQARIDRGEEVIVGVNKYRAEAGMAIDILDIDNA
                     KVREQQVAGLERIRKTRDAARVRAALDALGDAARSGKGNLLALAIEATRARATVGEIS
                     EALEKVFTRHRATIHSVSGVYGALYEGDESYRRVQREIETFARGEGRRPRMLVVKLGQ
                     DGHDRGAKVIATAFADIGFDVDIGPLFQTSEEAARQAIENDVHVVGVSSQAAGHKTLV
                     PELIAALKKAGGEDILVVCGGVIPAQDHEFLKRAGVAAIYGPGTNIPAAAGEILQLIA
                     KKRLAA"
     gene            1626..>2444
                     /locus_tag="VGP50_03380"
     CDS             1626..>2444
                     /locus_tag="VGP50_03380"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8026444.1"
                     /translation="MDRYLWPAMAILFFIVFIDLLGFGVMIPQLPFYGLHFGLHGFGV
                     ATMLACYSVAQFVSSPLLGRLSDRVGRRPVLLVSLAVSALAYCWLGFADAAWMLFAAR
                     LIAGAGAGNIAAAQAYIADVTPPERRAKGMGMIGAAFGLGFTVGPALGGLAARLDPAA
                     PAFIAAGLSVIAFLLTLARLKESLPEASRNAPPRPGRLAVAREALSRPVLRELILLLF
                     VTIGAFAGMETTFALWANQVFGWGSWEVGLNFFFVGVVLVVVQGGLIGWLSRRLG"
BASE COUNT          377 a          816 c          801 g          450 t
ORIGIN      
        1 atctatccgc cggccccgtc gatgcgcatc gttgccgaca tcatcgagta cacggcgaaa
       61 cacatgccga aattcaactc gatctcgatc tcgggctatc acatgcagga agcgggcgcg
      121 acagccgtgc aggaactcgc cttcacgctg gccgatggcc tcgactatgt gcgagcggcg
      181 ctgtcgaagg ggctcaagat cgacgatttc gcgccgcggc tctcgttctt tttctgcatc
      241 ggcatgaatt tcttcatgga gatcgccaag cttcgcgccg cgcgctatct ctgggcgaag
      301 ctcatcgcgc cgttccatcc cgcgaatccc gcgagtctcg cgttgcggac gcattgccag
      361 acctcgggcg tcagcctcac cgcgcaagac ccctacaaca acgtggtgcg cacgacgatc
      421 gaagcgctcg ccgccgtgct cggcggcacg cagtcgctgc acacgaactc cttcgacgaa
      481 gcgctggcgc tgccgacgcc gcagtcggcg cgcatcgccc gcaacaccca gctcatcctc
      541 gccgaggaga cgggcatcac tcatgtcgtc gatcccttgg gcggcagcta ctatgtcgag
      601 agcctcacgc attcgctggt ggcagcggca gaggcgctga tccgcgaagt cgaatctctg
      661 ggcggcatga cgaaagccgt cgagagtggt atgcccaagc tcaggatcga agaggcggcg
      721 gcgcggcgcc aggcgcgcat cgatcgcggc gaggaagtga tcgtcggcgt gaacaagtac
      781 cgcgctgaag cgggaatggc aatcgacata ctggacatcg acaacgccaa ggtgcgcgag
      841 cagcaagtcg ccggtctgga gcgcatccga aaaactcgcg atgcggcgcg cgtgcgggcg
      901 gcgctcgatg ccctcggtga tgccgcgcgg agcggcaagg gcaatctcct tgccctcgcc
      961 atcgaggcga cgcgcgcgcg cgccacggtc ggcgagatct cggaagcgct cgagaaggtg
     1021 ttcacgcgcc atcgcgcgac gatccactcg gtctccggcg tctatggtgc gctctacgag
     1081 ggcgatgaaa gctatcgccg cgttcagcgc gagatcgaga ctttcgcgcg cggcgagggc
     1141 aggcgaccgc gcatgctggt ggtgaagctc ggtcaggacg gccatgaccg cggcgccaaa
     1201 gtgattgcca cggccttcgc cgatatcggc ttcgacgtcg acatcggtcc gttgttccag
     1261 acgtccgaag aggccgcacg tcaggcgatc gagaacgacg tccatgtcgt gggcgtgtcg
     1321 agccaggccg cgggccacaa gacgctggtt cccgagctga tcgccgcgct caagaaggcg
     1381 ggtggcgagg acattctcgt cgtctgcgga ggagtcatcc cggcgcaaga ccacgagttc
     1441 ctgaagcgcg cgggggtggc ggcgatctat gggcccggca ccaacatccc cgccgctgcc
     1501 ggcgagatcc tgcaactcat cgccaagaag cgcctcgcag cttagttctc tttccccggc
     1561 ggagcgcgga cggcctctct tcgttcccgt ccggaagaaa gaaagtcaca ctggccgatc
     1621 gcgtcatcga tcgctatcta tggccggcca tggccatcct cttcttcatt gttttcatcg
     1681 atctgctggg cttcggggtg atgatcccgc agctgccatt ctatggcctg catttcggcc
     1741 tgcacggctt cggtgtcgcg accatgctgg cctgctactc ggtggcgcag ttcgtctctt
     1801 cgccgctgct cggacgcctc agcgaccgcg tcggccgccg ccccgtgctc ctcgtcagcc
     1861 tcgccgtttc cgctctggcc tattgctggc tgggcttcgc cgatgctgcg tggatgctgt
     1921 ttgccgctcg cctcatcgcc ggcgccgggg ccggcaatat cgccgcggct caggcctata
     1981 tcgccgacgt gacgccgccc gagcgccggg ccaagggcat gggaatgatc ggcgcggcgt
     2041 tcggcttggg gttcaccgtc gggccggcgc tcggcgggct cgccgctcgc ctcgaccctg
     2101 cggcgccggc gttcattgcc gccggcctct cggtgatcgc attcctgctg acgcttgccc
     2161 gtttgaagga aagcctgccc gaggcgtcgc gcaatgcgcc gccgcggccc gggcggctgg
     2221 ccgtggcgcg cgaagctctg agccggccgg tgcttcgcga gctcatcctt ctgctcttcg
     2281 tgaccatcgg ggcattcgcc ggtatggaga ccaccttcgc gctctgggcc aaccaggtct
     2341 tcggctgggg ctcctgggaa gtgggcctca actttttctt cgtcggcgtc gtcttggtgg
     2401 tggtgcaggg cgggctgatc ggttggctgt cgcgccgcct cggc
//
LOCUS       DASYNS010000106         5050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_804757, whole genome shotgun sequence.
ACCESSION   DASYNS010000106 DASYNS010000000
VERSION     DASYNS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804757"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VGP50_03385"
     CDS             <1..274
                     /locus_tag="VGP50_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8026445.1"
                     /translation="QSKSMSLLAALSADLADAKERRRVISGAKVQIGQSGRFIGQSSI
                     QLHGGMGMTDEYQAGHYFKRLTMIDQTFGDVDHHLDRFAEVTLPKT"
     gene            292..1092
                     /locus_tag="VGP50_03390"
     CDS             292..1092
                     /locus_tag="VGP50_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEV8026446.1"
                     /translation="MKIGAQALEEFHELGYLFLPDCFTAAEAALLRDEAERIYASSRQ
                     EVWREKTGAPRTAFAAHTYNEAFRLLGAHPRLIEPVEQILGEPVYMHQFKVNAKAAFE
                     GDVWQWHQDYGTWARDDGMPEPRAMNIAVFMDEVMAINGPLMLVPKSHRHGTLAAAHD
                     LATTSYPLWTLDKNSVTRLVEQGGIVAPTGKPGAVLLFHGNLVHGSAPNITPYPRRIV
                     YLTLCAVSNHIRKPSRPDWIAHRDFTPIVPVADDALLAHARHKRVAAE"
     gene            1307..2623
                     /locus_tag="VGP50_03395"
     CDS             1307..2623
                     /locus_tag="VGP50_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV8026447.1"
                     /translation="MSLYHLLQQRGRPVRAALVGAGKFGAMFLAQVPTTPGIEIAVIA
                     DLDPERARATCRSVGWDAPRIERTTFLTSGAEACAHPDVEVVVEATGSPAAGIAHAHA
                     AIDARKHVVMVNVEADVLAGPLLAAEARERGVVYSMAYGDQPALTCELVDWARAAGFA
                     VVAAGKGTKYLPAYHAVTPDDVWAHYGLTPEGALSGGMNPQMFNSFLDGTKSAIEMAA
                     IANATGLAVPADGLGFPPCGVDDLPQVLRPKSAGGVLAQEGMVEVVSCLERDGRPVFR
                     DLRWGVYVVLKAPTAYARACFAQYGLKTDASGWYAAMYKPYHLIGLELGISVLSAALR
                     REPTGETREFRGDVAAIAKRALKPGETLDGEGGYTVWGKLIPASRSLAESALPIGLAH
                     HVKLTRDVASGQMLRWSDVAAPDSEALRVRRAMERCFSPSAGAAVE"
     gene            2695..3030
                     /locus_tag="VGP50_03400"
     CDS             2695..3030
                     /locus_tag="VGP50_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV8026448.1"
                     /translation="MDKQPCVYIMASQRNGTLYVGVTSDIIRRAWEHRTDVVDGFTRG
                     YGVHRLVFIEFHDSMEAAIIREKRIKKWRRVWKLELIERANPQWNDLYDDLLSWRWPL
                     GPAFAGMTI"
     gene            3146..5047
                     /locus_tag="VGP50_03405"
     CDS             3146..5047
                     /locus_tag="VGP50_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV8026449.1"
                     /translation="MLSLVDVTYRIGGKTLLENTSAQINAGWKVALVGRNGAGKSTLL
                     ELIRGTLQTDGGTVLLPKDMRIGLVAQEAPGGETTPLAAVLAADEERARLLAAADAES
                     DGADTAEIHDRLHAIDAHGAPARAATILHGLGFDHEAQQQPLGQFSGGWRMRVALAAA
                     LFAEPDLLLLDEPTNHLDLEAALWLGEFLRRYRRTLLLVSHDRQFIDEIADHVLHLAE
                     RRLTLYSGGYETFLRTRREALARQQSLAAKQETQRRHLQAFVDRFRAKASKASQAQSR
                     LKMLARLEPVTLAEAEPQARFDFPQPTDLAPPLLTLDAVAVGYTPDRPVLRHLDLRLD
                     PSDRIALLGANGNGKSTLARLLAGRLEPLEGRVVRAPRLRSGYFAQHQIEEFDPGASA
                     FDHLSRLMPKASREAVRAGLARFGFDANTVFVAARDLSGGEKARLNFALIAREAPPLL
                     ILDEPTNHLDIAAREALVEAINEFAGAVVIISHDWHLLSLTAERLWLVADGTARPWDG
                     DLDDYRRYLLAERPTAAPGAERASSGVKRQGRRSGASRRDALAPLKHRLSAAEAELAK
                     LAREKAALDGRLADPATYGTRGPTLSELLRAQAEAAQSLAAAEARWLAAAEAVERAES
                     GENAAPQGG"
BASE COUNT          794 a         1844 c         1635 g          777 t
ORIGIN      
        1 gcagtcgaaa tcgatgtcgc tcctcgccgc gctctcggcc gacctcgcgg acgccaagga
       61 gcgccgccgc gtcatttccg gcgccaaggt gcagatcggg cagtccggcc gcttcatcgg
      121 ccaatcctcg atccagctcc acggcggcat gggcatgacc gacgaatatc aggccggcca
      181 ttatttcaag cggctcacca tgatcgacca gaccttcggc gacgtcgatc accacctcga
      241 ccgcttcgcc gaggtgacgt tgccgaagac gtgagcggcg ggaggcgcgt catgaagatc
      301 ggcgctcagg cgctcgagga attccacgag ctcggctacc tctttcttcc cgactgcttc
      361 accgcggcgg aggcggctct tttgcgcgac gaggcggagc ggatctacgc ctcgagccgc
      421 caggaagtgt ggcgcgagaa gaccggcgcg ccgcgcaccg cctttgccgc ccacacttat
      481 aacgaagcgt ttcgcctcct cggcgcgcat ccgcggctga tcgagcccgt cgaacagatc
      541 ctgggcgagc cggtctacat gcaccagttc aaagtcaacg cgaaagcggc cttcgagggc
      601 gatgtctggc agtggcacca ggattacggc acttgggcgc gcgatgacgg catgcccgag
      661 ccgcgcgcca tgaacatcgc cgtgttcatg gacgaagtga tggcgatcaa cggcccgctg
      721 atgctggtgc cgaagagcca ccgccacggc acgctcgccg cggcgcacga cctcgcgacg
      781 acgtcctatc cgctctggac gctcgacaag aactcggtga cgcgtctcgt cgagcaaggc
      841 ggcatcgtcg cgccgaccgg caagcccggc gccgtgctct tgttccacgg caacctcgtg
      901 catggctccg cgcccaacat cacgccttat ccgcgccgca tcgtctatct gacgctctgc
      961 gccgtctcga accacatccg gaagccctcc cgccccgact ggatcgccca ccgcgatttc
     1021 accccgatcg tgccggtcgc cgacgacgcg ctcctcgctc acgcgcgcca caagcgcgtc
     1081 gccgcggagt aagagcaaga gcgcaaagag cccctcgccc gcatcgcggg agagggaggg
     1141 gcccactgcg aagcttcagc gaagcagtgg gagggtgagg ggaccgcgca gaaaaatctc
     1201 cgatgttgac gccgctgcgc cgccccctca ccctcccatc gctaacgcga tgggtccctc
     1261 cctctcccgc gatgcgggag aggggcttgg tcccgcgagg aattgcatga gcctctacca
     1321 cttgctgcag cagcgaggcc ggcccgtgcg cgcggccctc gtcggcgccg gcaagttcgg
     1381 cgcgatgttc ctggcgcagg tgcccacgac gccggggatc gagatcgccg tcatcgccga
     1441 tctcgacccg gagcgggcgc gcgccacctg ccgcagcgtc ggctgggacg cgccgcgcat
     1501 cgagcgcacg acctttctca cctcgggggc cgaagcctgc gcgcatcccg atgtcgaggt
     1561 cgtcgtcgag gcgaccggct cgcccgcggc cggcatcgcc catgcccacg cggcgattga
     1621 cgcgcggaag cacgtggtga tggtgaatgt cgaggccgat gtgctggcgg ggccgctgct
     1681 cgccgcggaa gcgcgcgagc gcggcgtcgt ttattcgatg gcctatggcg atcagccggc
     1741 gctcacttgc gagctcgtcg actgggcgcg cgccgcgggc ttcgccgtcg tcgccgccgg
     1801 caagggcacg aagtacctgc cggcctatca tgcggtgacg cccgacgatg tctgggcgca
     1861 ttacggcctg acgcccgaag gggcgctgag cggcgggatg aacccgcaga tgttcaactc
     1921 ctttctcgac ggcaccaaat cggcgatcga gatggccgcg atcgccaacg ccaccggtct
     1981 cgccgtgccg gcggacggcc tcggctttcc gccttgcggc gtcgacgacc tgccgcaagt
     2041 gctgcggcca aaatccgcgg gcggcgtcct cgcgcaagag ggcatggtcg aagtcgtgtc
     2101 ctgcctcgag cgcgatggcc ggcccgtgtt ccgcgacttg cgctggggcg tctatgtcgt
     2161 gctgaaggcg cccaccgcct atgcgcgcgc ctgcttcgcc caatatggcc tcaagaccga
     2221 cgccagcggc tggtatgccg ccatgtacaa gccctaccac ctcatcggcc tcgagctcgg
     2281 catctccgtt ctgtcggcgg cgttgcgccg cgagccgacg ggcgagacgc gtgaatttcg
     2341 cggcgacgtg gccgccatcg ccaagcgcgc gctcaaaccc ggcgagacgc tcgacggcga
     2401 aggcggctac accgtctggg gcaagctcat ccccgcttcg cgcagcctcg ccgagtccgc
     2461 gctccccatc ggcctcgcgc atcacgtcaa gctcacccgc gacgtcgctt ccggccagat
     2521 gctgcgctgg tccgacgtcg ccgcgcccga cagcgaagcg ctccgcgtcc gccgggcgat
     2581 ggagcgctgc ttctccccct ccgccggtgc cgccgtcgag tagccaatac gctcacccca
     2641 ctttatcgtc atccccgcga aagcggggac ccaggggcga gcgacgggca gaccgtggat
     2701 aagcagcctt gtgtttacat catggcgagc caacgtaacg gtactcttta tgttggcgtg
     2761 acgagcgaca tcatccgacg cgcgtgggag caccgaacag atgtcgtcga tggattcacg
     2821 cgcggctatg gagtacatcg gctggtgttc atagaattcc atgactcaat ggaagccgcg
     2881 atcatacgcg aaaagcgaat caagaaatgg cgacgcgtgt ggaagctcga gctcattgaa
     2941 cgggccaatc cgcaatggaa cgatctttac gatgatctgc tgtcgtggcg gtggcccctg
     3001 ggccccgctt tcgcggggat gacgatatag ggatagggta cgaaattctt ctccctcact
     3061 gtctcaccgc ctcactggtc aatcttcctt tcttcctctt catcatcttt agaaccgccg
     3121 ccgcgcgtgc tatgacgcgc ccgccatgct cagcctcgtc gacgtcacct accgtatcgg
     3181 cggcaagact ctgctcgaga atacctcggc gcagatcaat gccggctgga aggtggcgct
     3241 cgtcggccgc aacggcgcgg gcaagtcgac gctgctcgag ctgatccgcg gcacgcttca
     3301 gaccgacggc ggcacggtcc tgctgccgaa ggacatgcgc atcggcctcg tcgcgcagga
     3361 ggcgccgggc ggcgagacga cgccgctcgc ggccgtgctc gccgccgatg aggaacgcgc
     3421 gcggcttctc gcggcggccg atgccgagag cgacggcgcg gataccgccg agatccacga
     3481 ccgcctccac gccatcgacg cccatggcgc gccggcccgg gccgcgacga ttctccacgg
     3541 cctcggcttc gaccacgagg cgcagcagca gccgctcggg cagttctcgg gcggctggcg
     3601 catgcgcgtg gcgctcgccg ccgcgctctt cgccgagccc gacctgctgc tcctcgacga
     3661 gcccaccaat cacctcgacc tcgaggcggc cctctggctc ggcgagttcc tgcgccgcta
     3721 ccgccgcacc ttgctgctgg tgagccatga ccggcaattc atcgacgaga tcgccgatca
     3781 cgtcctccat ctcgcggagc gccggctcac gctctacagc ggcggctacg agacgtttct
     3841 gcgcacgcgg cgcgaggcgc tggcgcgaca gcagtcgctc gccgccaagc aggaaacgca
     3901 gcgccgccat cttcaggcct tcgtcgaccg cttccgcgcc aaggccagca aagcgagtca
     3961 ggcacagagc cgcctcaaga tgctggcccg gctcgagccg gtgacgctgg ccgaagccga
     4021 gccgcaagcg cgcttcgatt tcccgcagcc gaccgatctc gccccgccgc tcctgaccct
     4081 cgacgcggtc gcggtcggct acacgccgga ccgcccggtg ctgcgccatc tcgatctgcg
     4141 cctcgacccg tccgaccgca tcgcgctcct cggcgccaac ggcaacggca aatcgacgct
     4201 ggcgcgtctc ctcgccgggc gcctcgagcc gctcgaaggc cgcgtcgtcc gcgcgcccag
     4261 gctgcgctcc ggctatttcg cccagcacca gatcgaggag ttcgatccgg gcgcgagcgc
     4321 cttcgatcac ctgtcgcggc tcatgccgaa agcgtcccgc gaggcggtgc gcgccggcct
     4381 cgcccgcttc ggcttcgatg ccaatacggt cttcgtcgcc gcgcgcgacc tctcgggcgg
     4441 cgagaaggcg cggctcaact ttgcgctcat cgcgcgcgag gcaccgccgc tgcttatcct
     4501 ggacgagccg accaatcatc tcgacatcgc cgcgcgcgag gccctggtcg aggcgatcaa
     4561 cgagtttgcc ggcgcggtgg tgatcatcag ccatgactgg catctgctgt cgctcaccgc
     4621 cgagcggctc tggctcgtcg ccgacggcac ggcccgcccc tgggacggcg atctcgacga
     4681 ctaccgccgc tatcttctcg cagagcggcc caccgcggcg ccgggcgccg agcgcgcgtc
     4741 gagcggggtc aagcgccagg ggcggcggag cggcgcctcc cggcgcgacg cgctcgcccc
     4801 gctcaagcac cggctcagcg ccgccgaggc cgagctcgcc aagctcgccc gcgagaaggc
     4861 agcgctcgat ggccgcctcg ccgaccccgc cacgtatggg acgcgcggtc ccacgctcag
     4921 cgagctgttg cgcgcccagg ccgaggcggc gcagtcgctg gccgccgcgg aagcgcgttg
     4981 gctcgccgcc gccgaggctg tggagcgcgc ggagagcggc gaaaacgccg caccccaagg
     5041 gggctgatct
//
LOCUS       DASYNS010000107         4706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4549760, whole genome shotgun sequence.
ACCESSION   DASYNS010000107 DASYNS010000000
VERSION     DASYNS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4549760"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VGP50_03410"
     CDS             <1..360
                     /locus_tag="VGP50_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026450.1"
                     /translation="RILALTKWHWSYSISLLEEAVARKLGFGLYGHQFGSPGVLIGHG
                     EEHHRYLLYVSERNYLRRIFCRPDAGDLSRGIGDLTIAELREAQTRRSFPRAASPMDF
                     SAGHTAIEEYAASAKVP"
     gene            complement(391..1440)
                     /locus_tag="VGP50_03415"
     CDS             complement(391..1440)
                     /locus_tag="VGP50_03415"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEV8026451.1"
                     /translation="MIPATHAVQRPADAKLLVIDSLGDVKTLARSRWTSFLRPGDLVI
                     ANDAATLPASLTGRHAPTGAAVEIRLAGRASLDAEDVTFQAVIFGDGDYRTRTEDRPP
                     PPAVDAGDVIELGPLTARIATILGHPRFVLLDFAGDAGGVWAGIARHGRPVQYAHMQE
                     PLRLWDVWTAIAGAPVAFEPPSAGFILTWAALRAIRARGATFATVTHAAGLSSTGAAA
                     LDMRLPLPEPYRIAPATADALRRARRVIAIGTTVTRALEHAGRSGAVHAGTGLADQRI
                     GADTKLEIVDAILSGTHEPDSSHYELMRAFADDRALARAAALMAQEGFRTHEFGDSVL
                     IERRRSAARDRHHAA"
     gene            complement(1437..2144)
                     /locus_tag="VGP50_03420"
     CDS             complement(1437..2144)
                     /locus_tag="VGP50_03420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8026452.1"
                     /translation="MTDKEDLRVAITGGTSGLGLALLRRFRSAGAAVAFTARSPQRVA
                     EIARAHPGAYGIVADVADKEMTYPTATQIGGCLGGLDVLINNASSLGPTPLALLADTS
                     CEEFARAIDTNLLGPFRLTKALLGLLAAAARERRGGVIVNISSDAAVTPYESWGAYGA
                     SKAALHHLSRIWERELAPEGIRLLSLDPGDMDTPLHALALPDADRAALKQPGDAAEEI
                     FAAIAAALPQRQPETAW"
     gene            2234..2809
                     /locus_tag="VGP50_03425"
     CDS             2234..2809
                     /locus_tag="VGP50_03425"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8026453.1"
                     /translation="MDQPVPAAAHLGRNLSTLRRTRGLTQAALAKAADLPRSTIANLE
                     SGEGNPSLVVLVKVSDALGVPVDELLASGRARVRLWRAAEVAEERAGHGLKTRPLVPE
                     PAPDETMTIMSFEPGGALKGTPHLPGTREYFTCLDGRVLIGVAGERFELAPGDVLGFP
                     GNLPHAYQNPDRRAGARGVSVVILAKATRDA"
     gene            2947..3822
                     /locus_tag="VGP50_03430"
     CDS             2947..3822
                     /locus_tag="VGP50_03430"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEV8026454.1"
                     /translation="MHWTDRRRRFRAILEGKRCVHPASVFDPISDRIAEDLGFELGML
                     AGSVASLAVLGAPDIILLTLSEFAAQAYRIGRAGTLPLLVDADHGYGNALNVMRTVDE
                     LETAGVAALTIEDTELPAAFGADKPRLVSIEEGVGKMRAAIVARQDRELAVVGRTSAA
                     SLSGIDDAVPRARAYAASGVDALFFVGIKKRGELDAIAAAVKVPIILGGAGAELEDAE
                     YLASRGVRIALQGHLPIMAAVQAVYEAMKALRSGARPASIPGIAAPELMQQVTRDRTY
                     KSWADAFLKPAGGQG"
     gene            3819..>4706
                     /locus_tag="VGP50_03435"
     CDS             3819..>4706
                     /locus_tag="VGP50_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEV8026455.1"
                     /translation="MSRKIAIVGAGAVGGYCGAHMVQAGEDVTFIDPWPAHVETMRRD
                     GLRISHAMDVPEFTVPVRALHVTDVQQFAKEAPVEVAFICVKSYDTAWATMLIKPYLA
                     PSGYVVSLQNCMNEETIAGIVGWGKTLGCIASRITAELRAPAHVHRGAGKGGARHTVF
                     RAGEAHGRVTPRAEEVCRLVGHADSAKTTENLWGERWSKLVANVMANGVSASTGLGSN
                     DCMTDETLRRFMLRLGSEAIRVGEALGYRLEEIFHLAPETIARAGEGDATAFAAASGR
                     LLSGRKHLSAEQRPSMAQDI"
BASE COUNT          729 a         1600 c         1646 g          731 t
ORIGIN      
        1 cgcatcctgg cgctcacaaa gtggcattgg tcctattcga tctcgctgct cgaggaagcg
       61 gtcgcgcgca agcttggctt cggtctctac ggccatcaat tcggctcacc cggggtgctg
      121 atcggccacg gcgaggaaca ccaccgctat ctgctctatg tctccgagcg aaattacctt
      181 cgccggatct tttgccggcc ggacgcgggc gatctatcgc gcggtatcgg cgacctgacc
      241 atcgccgagc tgcgcgaggc gcagacccgg cgcagcttcc cgcgggccgc gagtccgatg
      301 gatttttccg ccggccatac cgcgatagaa gaatatgcgg caagcgccaa ggtgccatga
      361 tcgctcactg tcggcgcgcg cggcgatgga tcacgcggcg tggtgtcggt cccttgctgc
      421 cgaccggcgt cgctcgatca ggacggaatc gccgaactcg tgggtgcgaa agccttcttg
      481 agccatgagc gccgccgcgc gggcgagcgc gcgatcatcg gcgaaggcgc gcatgagctc
      541 atagtgactg ctgtccggct catgagtccc ggaaaggatc gcatcgacga tttcgagctt
      601 ggtatcggct ccgatccgct ggtcggcgag cccggtaccc gcatgcacgg cgccgctccg
      661 ccccgcatgt tcgagcgcgc gtgtcaccgt cgtaccgatc gcgatgacgc gacgggcgcg
      721 gcggagggcg tctgcggtcg ccggcgcaat gcgatagggc tcgggcaacg gcaggcgcat
      781 gtcgagcgca gcagcgccgg tcgaagagag accggccgcg tgggtcacgg tcgcgaaggt
      841 cgcgccacgt gcgcggatgg cgcgtagcgc ggcccaggtc agaatgaagc cggccgacgg
      901 cggttcgaac gcgaccggcg cgccggcaat ggcggtccag acatcccaga gccgcagcgg
      961 ctcctgcata tgcgcgtatt gcacgggacg gccgtggcgc gcgattccgg cccacacgcc
     1021 gccggcgtcg ccagcgaaat cgagcaagac gaagcgcgga tggccgagaa tggtcgcgat
     1081 gcgtgccgtg agcggcccga gctcgatcac gtcgcctgca tccaccgccg gcggcggtgg
     1141 gcgatcctcg gtacgggtcc tgtagtcacc gtcgccgaag atgacggcct ggaaggtcac
     1201 gtcctccgca tcgagcgacg cgcgacctgc aaggcgaatc tcgaccgcgg ccccggtggg
     1261 cgcgtggcgc ccggtgagac tggccggcag ggtcgccgcg tcattggcga tgacgaggtc
     1321 gcccggccgg aggaagctcg tccaccggct gcgcgcgagc gtcttcacat cgcccaggga
     1381 atcgatgacc aacagcttcg catcggccgg tcgctggacc gcgtgcgtgg cggggatcac
     1441 cacgcagtct ccggctgtcg ctgcggcagc gcggcggcga tcgcggcaaa gatttcttct
     1501 gcggcgtcac cgggctgctt gagcgcggcg cgatcggcgt ccggcagcgc aagcgcgtgg
     1561 agcggcgtgt ccatgtcgcc cgggtcgagc gagagcaggc ggatgccctc gggcgcgagc
     1621 tcgcgctccc agatgcggct caggtgatgg agcgccgcct tgctggcacc gtaagcaccc
     1681 cagctctcat agggcgtgac ggcggcatcg gacgagatgt tgacgatcac gccgccgcgc
     1741 ctctcgcgcg ccgccgccgc cagcagaccg agcagcgcct tggtcagtcg gaacgggccc
     1801 agcagattgg tgtcgatggc gcgcgcgaat tcctcgcagc tggtgtccgc gagcagcgcc
     1861 agcggcgtcg gcccgaggct cgacgcgttg ttgatgagca cgtcgaggcc gccgagacat
     1921 ccgccgatct gtgttgccgt gggataggtc atctctttgt cggcaacgtc cgcgacgatg
     1981 ccgtaggcac ccggatgggc tcgggcgatt tcggcgacgc gctggggtga acgggcggtg
     2041 aaggccacgg cagcgccggc gctccggaag cgccgcagga gagcgagccc gagccctgac
     2101 gtgccgccgg tgatggccac gcgtagatct tctttgtcgg tcatgagagg tccctccctt
     2161 accgctcaga actgggcggg gccttgaccg cgcgcgagcg ccgtgtctga tataatggcg
     2221 gaaaggcacc aagatggacc agcccgtccc cgccgccgct catctcggcc gcaatctctc
     2281 gacgcttcgc cgcacccgcg gactgaccca ggcagcgctg gccaaggcgg cggacctgcc
     2341 gcgctcgacc atcgccaatc tcgaatccgg cgaaggcaat ccctcgctgg tggtgctggt
     2401 gaaagtgtcg gacgccctgg gcgtgccggt cgatgaattg ctggcgtcag ggcgcgcgcg
     2461 ggtgaggctc tggcgcgccg ccgaagtggc ggaggaacgg gccggtcatg ggctcaagac
     2521 gcgccctttg gtgccggagc cggcccctga cgagaccatg accatcatga gcttcgagcc
     2581 cggcggcgcg ctcaagggca cgccgcatct gcccgggacc cgcgaatact tcacctgcct
     2641 cgacggccgt gtcttgatcg gcgtggcggg cgagcgattc gagctcgcgc ccggcgacgt
     2701 cctgggcttt cccggcaatc ttccgcacgc gtatcagaac cccgaccggc gggccggcgc
     2761 gcgcggcgtc tcggtggtga tcctggcgaa agcgacgcgc gacgcgtagc gccgcgcgac
     2821 gcggagcgtc gtcgcgggcg ggcgccagcg gttgcgtcag caaccgcgaa agcccgcaca
     2881 gatcggagta gccgcgccca gcaaattccc tatcatcctc gctgtcacga gggagggctg
     2941 cacgttatgc actggactga ccgacgccgg cgctttcgcg ccatcctcga gggaaagcgc
     3001 tgcgtccatc ccgcctccgt gttcgacccg atctcggacc gcattgccga ggatctgggc
     3061 ttcgagctcg gcatgcttgc gggctcggtg gcgtcgctcg ccgtgctcgg cgcgcccgac
     3121 atcatcctgc tgacgctcag cgaattcgcg gcgcaggcct accggatcgg ccgcgccggc
     3181 actctgccgc tgctggtcga tgccgatcat ggctatggca acgcgctcaa cgtcatgcgc
     3241 accgtggacg agctcgagac cgccggcgtc gcggcgctca ccatcgagga caccgagctt
     3301 cccgccgcct tcggcgccga caagccgagg ctcgtctcga tcgaggaagg cgtcggcaag
     3361 atgcgcgcgg ccattgtcgc gcgccaggat cgcgaactcg ccgtcgtcgg gcgcacttcc
     3421 gccgcatcgc tcagcggcat cgatgacgcc gtgccccgcg ccagagctta tgcagcgtcg
     3481 ggcgtcgacg cgctcttctt cgtcgggatc aagaagcgtg gcgagcttga cgccatcgcc
     3541 gcggcggtga aagtgccgat cattctgggc ggtgccggcg ccgagctcga ggacgcggaa
     3601 tacctcgcgt cgcgcggcgt gcgtatcgcg ctccagggtc acctgccgat catggctgcc
     3661 gtgcaggccg tgtacgaggc gatgaaggcg ttgcgctcgg gcgcgcgtcc ggcgtcgatc
     3721 cccggcatcg ccgcgcccga gctcatgcag caggtcacgc gcgatcgcac ctacaaaagc
     3781 tgggccgatg cgttcctgaa gccggcagga ggacagggat gagccggaag atcgcgatcg
     3841 ttggcgctgg cgccgtcggc ggctattgcg gcgcccatat ggtgcaggcg ggcgaggacg
     3901 tgaccttcat cgatccctgg ccggcgcatg tcgaaaccat gcgcagggac ggattgcgca
     3961 tcagccacgc catggacgtg ccggaattca cggtgccggt gcgcgccctt cacgtcaccg
     4021 acgttcagca attcgccaaa gaggcgccgg tcgaggtggc cttcatttgc gtcaaatcct
     4081 acgacaccgc ctgggcgacg atgctgatca agccgtatct ggccccgtcg ggctatgtcg
     4141 tctccctcca gaactgcatg aacgaggaga ccatcgccgg catcgtcggt tggggcaaga
     4201 ccctgggctg catcgcgagc cggatcacgg cggagctgcg cgcgccggcc catgtccatc
     4261 gcggcgccgg caagggcggc gcgcggcaca ccgtgtttcg cgcgggcgag gcgcatgggc
     4321 gcgtcacgcc ccgcgccgag gaagtgtgcc gcctcgtcgg ccatgccgac agcgccaaga
     4381 cgacggaaaa tctctggggc gagcgctggt cgaagctcgt cgccaacgtc atggcgaacg
     4441 gcgtctccgc atccaccggc ctcggcagca acgactgcat gaccgacgag actctgcggc
     4501 gcttcatgct gcgtctcggc agcgaggcga tccgcgtcgg cgaagccttg ggctatcgcc
     4561 tcgaagagat cttccatctc gcgcccgaga cgattgcgcg cgccggcgag ggcgacgcga
     4621 ccgctttcgc cgccgcctcc ggccggctgc tctccgggcg gaagcatctc tccgccgagc
     4681 agcgtccctc catggcccag gacatc
//
LOCUS       DASYNS010000108         4098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2053691, whole genome shotgun sequence.
ACCESSION   DASYNS010000108 DASYNS010000000
VERSION     DASYNS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053691"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..887
                     /locus_tag="VGP50_03440"
     CDS             57..887
                     /locus_tag="VGP50_03440"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEV8026456.1"
                     /translation="MTLLPRHPAPPPHRRRTWAALVVIGVAAAVPILMAPAGRGARAT
                     IDRCAAPADLAALGAPLPRTAARLAARHPLTIVALGSSSTEGVGATRPELNYPSRLSA
                     WLGARFPGVPIHVINRGVSGEMTPQMLARLDRDVLAEKPDLVIWQLGTNDIFHDIDPA
                     DETEFARRGIERIRAAGADVILMDLQYAPAVLVHAGYRDTLRALAGVARSEDVALFHR
                     FAVMRDWAEEGRMTLAVMLSADRLHMSDASYDCLARQLARGIAGAVRPAAVDLAARDD
                     "
     gene            complement(1020..1280)
                     /locus_tag="VGP50_03445"
     CDS             complement(1020..1280)
                     /locus_tag="VGP50_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEV8026457.1"
                     /translation="MDNENERRIRERAYDIWQREGRPHGRDAEHWRKAAAEIEAESRP
                     RPEPETVGAGSAPLRSRRAAKPARNAEPATPPAPSPKRPAGR"
     gene            complement(1323..1652)
                     /locus_tag="VGP50_03450"
     CDS             complement(1323..1652)
                     /locus_tag="VGP50_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026458.1"
                     /translation="MVRPGENYSRVRRSIARRRRAALLYGLTGALVFAAMPSSAGEEP
                     AAAAVPQVAVATTAEPEGSTTPPDVTVVKEPEPGAERTSDQQRRVLMLLIMNSAGPVR
                     PFGNLGR"
     gene            complement(1772..2329)
                     /locus_tag="VGP50_03455"
     CDS             complement(1772..2329)
                     /locus_tag="VGP50_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEV8026459.1"
                     /translation="MRSTIRIALAALAIVLPLAACSQGSSLPPLEASKTDDANYRLGP
                     GDKLHMQVLGAEDLTGDYTVGDNGTVSSPLIGDVKAAGLTRGQLERELERKLAQGYLR
                     NPKVSVTILAYRPFYIYGEVTKPGEYPYASGMRVMSAVATAGGFTYRANESYVVVTRD
                     GKEHKAEPNTPIQPDDVIRVPERYF"
     gene            2561..3394
                     /locus_tag="VGP50_03460"
     CDS             2561..3394
                     /locus_tag="VGP50_03460"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV8026460.1"
                     /translation="MTPVAKALWLIEHRSASDLSLDEIARLCDVSRFYMSRAFVLATG
                     HSVMRYLRARRLTEAARALAAGAPDILAVALDAGYGSHEAFTRAFRDRFGMTPDAVRS
                     RRHLDNLATVEAIRMDGKLLVDLAPPRVENGKALLVAGLAARYTFGSTEGIPAQWQRF
                     AQYLGHIPGQVGDTRYGVSCNHDDAGHFDYITGVEVADFADFADEFARVRIPAQRYAV
                     FSHREHIAGIRGTIYTIWNKWLPESGHQVADAPNFERYGPDFDPRTGTGVVEIWVPIK
                     A"
     gene            complement(3418..3846)
                     /locus_tag="VGP50_03465"
     CDS             complement(3418..3846)
                     /locus_tag="VGP50_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026461.1"
                     /translation="MAEQWQYQLRIDLGDAAAEAARRGPDDPAVKPLAAILARHRATM
                     KSQYDAFAEYVAAAEAQGRDKFPLYEWTKTTIENPAKKEKYLKSFTLYVDGNEVYDKA
                     AADALEMDLRPLVGGALIARMSKYDTNPANNPQPPVRPRG"
BASE COUNT          624 a         1386 c         1334 g          754 t
ORIGIN      
        1 gccgtttatg aatgcattgc acaacgcctc gccgacgcga tcgagtatgc ggtgaaatga
       61 cattgcttcc gcgccatccc gctccgccac cgcatcgccg ccgcacctgg gcagcgctcg
      121 tggtgatcgg tgtcgccgcc gcggtaccca ttctcatggc cccggcaggc cgtggcgcgc
      181 gggcgaccat cgaccgttgc gcggctccgg cggacctcgc cgcattgggt gcgccgctgc
      241 cgcgtaccgc cgcgcgcctt gccgctcggc acccgctcac catcgtggcg ctgggctcgt
      301 cctcgaccga aggcgtgggt gccacgcggc ccgagctcaa ttatccgagc cgtctctcgg
      361 cctggctcgg ggcgcgcttc ccgggcgtgc cgatccatgt catcaaccgc ggcgtcagcg
      421 gcgagatgac cccacagatg ctggcgcgcc tcgatcgcga cgttctggcg gagaagcccg
      481 accttgtgat ctggcagctc ggcaccaacg acatctttca cgatatcgac ccggccgatg
      541 aaacggagtt cgcccgccgc ggcatcgagc gtatcagggc agcgggcgcc gacgttattc
      601 tcatggatct gcaatacgcg ccggccgtgc tggtccacgc cggctatcgc gacacgctgc
      661 gcgcgctggc gggcgtcgcc cgcagcgaag acgtggcgct cttccaccgt tttgcggtga
      721 tgcgcgactg ggccgaggag ggccgcatga cgctcgccgt catgctgtcg gcggatcgct
      781 tgcacatgtc ggacgcaagc tatgattgcc ttgcgcgaca gctggcgcgg ggcatcgcgg
      841 gtgccgtccg tccagccgcc gtcgatctgg cggcgcgcga cgactgacat aagatagccc
      901 gcaccacctt cgagtgatgc gggcgagttt ccaatcccag tcgcactagg ctctgaacca
      961 acatgcggac gccgcaatgg ttccgtttcg gtaaaaccgt cgccggcgac gtgtcccgct
     1021 caacgccccg ccggccgttt gggcgatggc gccggcggcg tcgccggctc ggcgttgcgg
     1081 gcgggtttgg cggcacggcg gctgcgcagc ggtgcactgc cggcgccgac ggtttccggc
     1141 tccggtcgcg gccggctctc ggcctcgatc tcggctgcgg ccttccgcca atgctccgcg
     1201 tcgcgaccat gcgggcgacc ctcgcgctgc cagatgtcat aggcccgctc gcggatacgc
     1261 cgctcgttct cgttgtccat agcttgtctc gcgcctcgac ccactgcggg gaaaacgaac
     1321 gtctatcggc cgaggtttcc gaaaggccgc accgggccgg cgctgttcat gatgagaagc
     1381 atgagcacgc ggcgctgctg gtcgctcgtg cgttctgcgc ccggctcggg ttctttgacg
     1441 acagtaacgt caggtggcgt ggtgctgccc tcgggttctg ccgtcgttgc cacggcgact
     1501 tggggcacgg cggctgctgc cggctcctcc cctgccgatg acggcatcgc ggcaaaaacg
     1561 agggcgccgg tgagcccgta gagaagcgcg gcgcggcgcc gccgagcgat cgatcgccga
     1621 acgcgggagt agttctcgcc ggggcggacc atggtcccct cactgcgctt tcgcgctgtc
     1681 caatgcagcg acgatcgatg cgcttctcac tgagaaatac ggcgcaattt cgatatggtt
     1741 cccccaaaat gcgccgctgc gtcgcagcat ctcagaaata gcgttccggc acgcgaatga
     1801 cgtcgtcggg ctggatgggt gtgttcggct cggccttgtg ctccttgccg tcgcgggtca
     1861 ccaccacgta actctcgttg gcgcgatagg tgaagccgcc ggcggtagcc acggcgctca
     1921 tcacgcgcat gccgctggca taaggatatt cgccgggctt ggtcacctcg ccatagatgt
     1981 agaaggggcg ataggcgagg atggtgacgc tcaccttcgg gttccggagg tacccttggg
     2041 cgagtttccg ctccagctcg cgctcgagct ggccgcgggt cagtccggcc gctttgacgt
     2101 cgccgatcag cggcgagctc acggttccgt tgtctcccac ggtatagtcg ccggtcagat
     2161 cctcggcgcc caggacctgc atatgcagct tgtcgccggg cccgagccgg taattggcat
     2221 catccgtctt gctcgcctcg agcggcggca ggctcgaccc ttgagagcag gccgcgagcg
     2281 gcagcacgat cgccaacgcc gcgagggcga tgcggatggt cgaacgcatg gcagtttctc
     2341 tcccgttcat caccgacggc gtggaacgac gagcgaacga caaataacgc tcaacgttcc
     2401 atgggcggga cgatctcgcc cttttggttg acgatgacgc gcctccgttc tagggacctt
     2461 aacgcgcgcc ggcaagccga tttgccgacg aacggcaagc ccgatcaaat gagcggacgg
     2521 gaatactgac acgcttcgtc gggcttggct gggaacgacc atgacgccgg tggcgaaggc
     2581 tctctggttg atcgaacatc gctcggcgag cgacctttcg ctcgacgaga tcgcgcgtct
     2641 ctgcgatgtt tcccgcttct acatgtcgcg ggcattcgtc ctcgcgacgg gccactcggt
     2701 catgcgctat ctgcgcgccc gccgcctgac ggaggcggct cgcgcgctcg ccgccggagc
     2761 tcccgacatt ttggccgtag cgctcgatgc gggctacggc tcgcatgagg cattcacccg
     2821 ggcatttcgc gatcgcttcg ggatgacgcc cgacgccgtt cgcagccgac gccatctcga
     2881 caacctggca accgtggagg ccatcagaat ggacggaaag cttctcgtcg atctcgcgcc
     2941 gccgcgcgtc gaaaacggca aggcgctgct cgtcgccggc ctcgccgccc gctacacctt
     3001 cggcagcacc gagggcatcc cggcgcagtg gcagcgcttc gcgcaatatc tcgggcacat
     3061 cccgggacag gtcggcgaca cgaggtacgg cgtctcctgc aaccacgacg acgccggtca
     3121 cttcgactat atcacgggcg tcgaagtagc cgacttcgcg gatttcgcag acgagttcgc
     3181 gcgagtgcgg ataccggcgc agagatacgc cgtcttctct caccgcgaac acatcgccgg
     3241 catccgcggc acgatctaca ccatctggaa caagtggctg cccgaatccg gccatcaggt
     3301 ggcggatgcg ccgaatttcg agcgatatgg accggatttc gatccccgga ccgggaccgg
     3361 cgtcgtcgag atctgggtgc cgatcaaggc gtgatagcca gcgatctcgg ctcttcccta
     3421 gccgcggggc cgcaccggcg gctgcggatt gttagccgga ttggtgtcgt atttggacat
     3481 gcgggcgatc aacgcgccac ccaccaaggg gcgcaaatcc atttcgagcg cgtccgccgc
     3541 cgccttgtca tagacttcgt tgccgtcgac atagagcgtg aaggacttca gatatttttc
     3601 cttcttggca ggattctcga tcgtcgtctt ggtccattcg tagagtggaa atttgtcgcg
     3661 cccttgcgct tcggccgccg ccacatactc cgcgaaggca tcgtattggg atttcatcgt
     3721 cgcccgatgc cttgccagga tcgccgcgag cggtttgacc gccggatcgt ccgggccgcg
     3781 ccgtgccgcc tcggccgccg catcgccgag gtcaattctt agctgatact gccattgctc
     3841 ggccatgctc gacctcgggg cgtcggtggt gacgatgagc gactcttcgg cggcgtatcc
     3901 tattctgctc gggatggggc cgcaacgtcg acgcccgcgc cgctggccgg cgccgcgacg
     3961 gcgagtcggg ttaaggccgc agcaccgcgc ggcctgtgat gcgcccgccg cgcagctctt
     4021 cgagcacggc atttgcgtcg tcgagcggcc gctcgctcac cggcatgggc gcgaccttgc
     4081 cggtgccgac cagcgcca
//
LOCUS       DASYNS010000109         4753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4706169, whole genome shotgun sequence.
ACCESSION   DASYNS010000109 DASYNS010000000
VERSION     DASYNS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4706169"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VGP50_03470"
     CDS             <1..669
                     /locus_tag="VGP50_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026462.1"
                     /translation="QNYRAIEIGGALMWIALPQFVLAPIVATTLRFVDARFLLALGFA
                     LVGGACFMAGQLTHDWVGEDFLPSQLVQAAGQSLALTSVVWFSLKHLEPSEILTFGAM
                     LQTARLFGGELGSAFVQTFVRVREQVYSNLIGLHVTTGSLATDHRLQDYAQAVLGRSI
                     GQAEATARSTALLASAVRNQANVLAYIDGFMVIGFAAIGVLLLMLFLRAAPAQQSPPA
                     PATN"
     gene            801..1154
                     /locus_tag="VGP50_03475"
     CDS             801..1154
                     /locus_tag="VGP50_03475"
                     /inference="COORDINATES: protein motif:HMM:NF042576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6717 family protein"
                     /protein_id="HEV8026463.1"
                     /translation="MNVINVVEPYRHLEMWVFDDPRVGLSAEPFVGGADTMIDRVTAD
                     IPHADRGFVIVFSGIPFPGHQFRLEWRREEGTGNVYHSRELDAEGWLCPALLRYFEKR
                     PSEIFIQVKAKSVRT"
     gene            complement(1325..1401)
                     /locus_tag="VGP50_03480"
     tRNA            complement(1325..1401)
                     /locus_tag="VGP50_03480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1365..1367),aa:Arg,seq:acg)
     gene            1582..2820
                     /locus_tag="VGP50_03485"
     CDS             1582..2820
                     /locus_tag="VGP50_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026464.1"
                     /translation="MPIGKYLGVVLAASLLSGCSAFDGVWSTLAGGGSSTPSSGRVIA
                     IPTPKPDAAAQPAVTAGITVPAPAVVPADTTAAAPSIVPAGGAVHAVPSTPSAAALSG
                     PTTVAALSGGTAAGTPQAQGLRGDVQRLQEEIGRQNAESQRLHQALAQDSASYYGAVA
                     TIHSRLQIGTSPGNPELVAQWNLAQVALDRMSADIARLAVLSGEAASDSTLATQILDS
                     VKTGASSAGDPRQLQAVESDTRSAQGQVQLLVDAAAQDIARQTAYLDNERTNLVGLLT
                     AIKSGELYGRRLADGRYVAAFKATDVQREAALSGSSERKPLVVIRFRRPHVAYEGALY
                     RAMKTALERRPSASFDLVAVAPVANTPAEVDANGRAAKRDAATVLQSLTDMGLPAERV
                     SLSSTTSAQVRNDEVQIYVR"
     gene            2837..3331
                     /gene="gpt"
                     /locus_tag="VGP50_03490"
     CDS             2837..3331
                     /gene="gpt"
                     /locus_tag="VGP50_03490"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV8026465.1"
                     /translation="MTPSKTFPVSWDELHRYSRALAWRLIELGPWHSIIAVTRGGLVP
                     AAIVARELEIRLIDTLCIASRVAPTPGAIDSERSGITVLKRIPGDGTGQLIIDDLVDT
                     GDTARVVRDMLPKAHFATVYAKPAGRPLVDTFVTEVSQDTWILFPWDIEPQFVAPIAT
                     QRGG"
     gene            3552..>4753
                     /locus_tag="VGP50_03495"
     CDS             3552..>4753
                     /locus_tag="VGP50_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEV8026466.1"
                     /translation="MLALSLGAAAAQTPAAPEPATGRAAKQASLARHDMVVAANPLAA
                     SAGRDVLRQGGSAIDAAIATQLVLNLVEPQSSGIGGGAFLVLWSAKERRIVTFDGRET
                     APSAARPDRFIGADGKPLAFYDAVVGGRSVGVPGALRVLELAHQRYGKLPWAGLFAPA
                     IALAEQGFPVSPRLRTLLERDPYLRRDEPARSYFYAADGTPKTTLVNPDLAQTLRAIA
                     AGGADAFYRGAIAHDIVAAVATARSSPGDLSENDLAAYAAKERAPVCGRYRADRLCGM
                     GPPSSGAVTLLEMLGLLERFDLARDRPPSVEAVHLLAEAGRLAYADRARYLGDPEFVP
                     APIAGLLDGDYLKTRTLLIDPAHALPGPAAPGEPRGTHARLWGNDAAPELPSTSDVSI
                     VDRDGDALA"
BASE COUNT          738 a         1686 c         1601 g          728 t
ORIGIN      
        1 cagaactatc gcgccatcga aatcggcgga gccttgatgt ggatcgccct gcctcagttc
       61 gtgctggcgc cgatcgtcgc gacgaccctt cgcttcgtcg acgcccgctt cctgctggcg
      121 ctcggttttg cgctcgtcgg cggcgcctgc ttcatggcgg gtcagctcac ccatgactgg
      181 gtgggcgaag acttcctgcc ctcgcagctc gtccaagcgg ccggccagtc gcttgccctg
      241 acgtcggtgg tgtggttcag cctgaagcat ctcgagccga gcgagattct caccttcggc
      301 gcgatgctgc agacggcccg cctgttcggc ggagagctcg gcagcgcctt cgtgcagacc
      361 ttcgtccggg tgcgcgagca ggtctactcg aacctcatcg ggctgcacgt gaccacgggg
      421 tcgctggcga cggaccaccg gctgcaggac tacgcgcaag ccgtgctggg acgctcgatc
      481 ggccaggccg aggcgacggc gcggagcacg gcgctcctgg ccagcgccgt gcggaaccag
      541 gccaatgtgc tggcctatat cgacgggttc atggtcatcg gcttcgccgc gatcggcgtc
      601 cttctactga tgctgttcct tcgcgccgcg cccgctcagc agagcccgcc ggcccccgcg
      661 acgaattgaa gcgggtatgc gcgccatcga gcaggcgggc taccggccgg ccgagcatgt
      721 gagcctcgac ggctgcaagc cgctcgccga ccggttagaa ttggcccgca ggccgctgtg
      781 tcggcaaacc gagaaccgct atgaacgtga tcaatgtcgt agagccctat cgccacttgg
      841 aaatgtgggt tttcgatgac ccgcgggtcg gactttcggc ggagccgttt gtcggcggag
      901 ccgacaccat gatcgaccgt gttactgcag atattccaca tgctgaccgg ggattcgtca
      961 tcgtcttttc gggaattccc tttccgggcc atcagttccg gcttgaatgg agaagggaag
     1021 agggcaccgg gaatgtttac cattcccgcg aactcgatgc cgaaggttgg ctctgcccag
     1081 ccctgctccg ctattttgaa aagcggccgt ccgaaatctt tatccaggtg aaggcgaagt
     1141 cagtcagaac ctaacccgcc gcgtatccga cttaacggcg gcaactccac gaagaagcct
     1201 acgagccgga gcaaatgcaa tggcaaggta gcggcgcaac acgactctgt tctttgcagt
     1261 cctggaccga cgtggcgtta ggcaacgcga agacgggcat tacgaggaat gaccgccgaa
     1321 atattggcgc gcccggagag attcgaactc ccaaccccca tatccgtagt ctggtgctct
     1381 atccagttga gctacgggcg catgcgccgc tcggccgtcc gcggcggagg cggaacgtag
     1441 cggcaagccc tggccaaggc aaggcgggaa tgcccgcgcg ggcggtgaaa cgcgcttgtc
     1501 gcgaaaagca tccgtcccct agtatcggtc aagcattcgg ccatgccggc ggctcggccg
     1561 ccggcgagaa ggggcaagca catgccgatc ggcaaatatc tgggcgtcgt cctcgcagcg
     1621 tcgctgctct ccggctgctc ggccttcgac ggcgtctgga gcacccttgc gggcggcggc
     1681 tccagcacgc cgtcctcggg ccgcgtcatc gccatcccga cgccgaagcc cgacgcggcg
     1741 gcacaaccgg ccgtcacggc gggaataacg gtgcccgcgc ccgcggtcgt gcctgccgac
     1801 acgacggcgg cggcgccgtc gatcgttcca gcgggcggcg cggtgcacgc ggtgccatca
     1861 acaccctcgg ccgcggcgct ctccggcccg acgaccgtcg ccgccttgag cggcgggacc
     1921 gcagccggca cgccgcaagc ccagggcttg cgcggcgacg tgcagcggtt gcaagaggag
     1981 atcggccgcc agaatgccga gagccagcgc ctgcatcagg cgctggcgca ggacagcgcc
     2041 agctattacg gcgccgtcgc cactatccat tcgcgcctcc agatcgggac atcgccgggc
     2101 aatcccgagc tcgtcgcgca atggaacctg gcgcaggtgg cgctcgaccg catgagcgcc
     2161 gacatcgccc gcctcgccgt gctctcgggc gaagcggcga gcgattcgac gctcgccacg
     2221 cagatcctcg attccgtcaa aaccggcgcc agcagcgccg gcgacccgcg gcagctccag
     2281 gcggtcgaaa gcgacacgcg gagcgcccag ggacaggtgc agcttctcgt cgacgcggcg
     2341 gcgcaggaca tcgcgcgaca gaccgcctat ctcgacaacg agcgcacgaa cctcgtcggc
     2401 ctcctcaccg ccatcaagag cggcgagctc tatggccggc gcctcgcgga cggccgctat
     2461 gtcgcggcgt tcaaagcgac ggatgtccag cgcgaggccg cgctctccgg cagcagcgag
     2521 cggaagcccc tggtcgtgat ccgcttccgg cggccccatg tcgcttatga gggcgcgctc
     2581 tatcgcgcga tgaagacggc gctcgagcgc cgccccagcg cgagcttcga tctggtcgcg
     2641 gtggcgccgg tcgcaaacac gccggccgag gtcgatgcca atggccgcgc cgccaagcgc
     2701 gacgccgcga cggtgctgca gtcgctcacc gatatggggc ttccggccga gcgcgtctcg
     2761 ctctcctcga cgaccagcgc ccaagtcagg aacgacgagg tccagatcta cgtccgatga
     2821 agggcctggt ggcggcatga cgcccagcaa gacgttcccg gtgagctggg acgagctgca
     2881 ccgctattcc cgcgccttgg cctggcggct catcgagctc gggccctggc acagcatcat
     2941 cgccgtgacg cggggcggcc tcgtgcccgc cgcaatcgtc gcccgcgagc tcgagatccg
     3001 cctgatcgac acgctctgca tcgcgagccg cgtcgcgccg acgccgggcg ccatcgacag
     3061 cgagcgcagc ggcatcaccg tgctgaagcg catccccggc gacggcacgg gacagctcat
     3121 catcgacgac ctcgtggata ccggcgacac ggcgcgcgtc gtgcgcgaca tgctgcccaa
     3181 ggcgcatttc gcgaccgtct atgccaagcc tgccgggcgg ccgctggtcg acaccttcgt
     3241 caccgaggta agccaggaca cctggatcct cttcccctgg gacatcgagc cgcaattcgt
     3301 cgcgcccatc gctacacaac gcggcgggtg acggtccgtt tcccgtacgc caacggcgtt
     3361 ttgcgcggat gtctcgggtg ttggaggaga cattgatcaa gtcgtcattc ccgcgaaagc
     3421 gggaacccag gggcacccgg cgcgtcgctc gccccccgct tttcgcgggg gcaggctctg
     3481 ggtccccgct ttcgcgggga agacgaagaa ggaatggctc tgcatgggcg ggaggcgcgc
     3541 tcggtgcagc gttgcttgcg ctctcgctcg gcgccgcggc ggcgcagacg ccggcggcgc
     3601 cggagccggc gaccgggcgc gcggcgaagc aggcgagcct ggcgcggcac gacatggtcg
     3661 tagcggccaa tccgttggcg gcgagcgcgg gacgcgacgt gctgcgccag ggcggcagcg
     3721 ccatcgacgc cgccatcgcc acgcagctcg tgctcaatct ggtggagccg caatcatcgg
     3781 gcatcggcgg cggcgcgttc ctcgtgctgt ggtcggccaa ggagcggcgg atcgtcacct
     3841 tcgacggccg cgagaccgcg ccgagcgccg cgcggcccga ccgcttcatc ggcgccgacg
     3901 gcaagccgct cgccttctac gacgccgtcg tgggcgggcg ctcggtgggc gtgcccggcg
     3961 ccttgcgcgt cctcgagctc gcgcatcagc gctacggcaa gctgccctgg gcggggctct
     4021 tcgcgccggc aattgcgctc gccgagcaag gcttccccgt ctcgccgcgg ctgcgcacgc
     4081 tgctcgagcg cgacccttat ctccgccgcg acgagccggc gcgcagctat ttctatgcgg
     4141 cggacggcac gcccaagacc acgctcgtca acccggacct ggcgcagacg ctccgcgcga
     4201 tcgccgccgg cggcgccgac gccttctatc gcggcgccat cgcccatgac atcgtcgccg
     4261 cggtcgccac cgcgcgcagc agccccggcg atctcagcga gaacgatctc gcggcgtacg
     4321 cggcgaagga gcgggcgccg gtctgcggcc gctatcgcgc cgatcgcctc tgcggcatgg
     4381 gcccgcccag ctcgggcgcg gtgacgctcc tcgagatgct gggcctgctc gagcgcttcg
     4441 atctggcgcg cgaccgcccg ccctcagtcg aggcggtgca tctcctggcc gaagccggcc
     4501 gcctcgccta tgccgatcgc gcccgctatc tcggcgatcc cgaattcgtg ccggcgccga
     4561 tcgcggggct gctcgacggc gattacctca agacgcggac gctgctcatc gacccggccc
     4621 atgccttgcc gggaccggcg gcgcccggcg agccgcgcgg aactcatgcg cgactgtggg
     4681 gaaacgacgc cgcgccggag ctgccctcga ccagtgacgt ctcgattgtc gatcgcgacg
     4741 gcgacgcgct ggc
//
LOCUS       DASYNS010000110         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1743142, whole genome shotgun sequence.
ACCESSION   DASYNS010000110 DASYNS010000000
VERSION     DASYNS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743142"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VGP50_03500"
     CDS             complement(<1..239)
                     /locus_tag="VGP50_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV8026467.1"
                     /translation="MTRSVVFAEPVRTAIGTFGGSLKDVAAPDLGAAAIKAALARARL
                     RPEDIATVALGNVIQAGVKMNPARQAAIHAGLPVSV"
     gene            complement(271..1824)
                     /locus_tag="VGP50_03505"
     CDS             complement(271..1824)
                     /locus_tag="VGP50_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEV8026468.1"
                     /translation="MTAEPKHPSLLPFVVMCVGMFIALLDIQIVASSLQNIGGGLSAA
                     QDEISWVQTAYLIAEIIVIPLSGWLTRVFSTRWLFTISAAGFTAASMLCGIAWSIESM
                     ILFRALQGLLGASMIPTVFTSSFHFFQGPRRVYSAAVIGAIASVAPTLGPVIGGWITD
                     TLDWRWLFYVNLVPGLAITALVPMLVRIDKPDLALLRGADYVGIALMAIALGTLEYVL
                     EEGTRWNWFSDATIRDCAWISAIAGTLFVVRSLTRPEPVVDLRALRDRNFAIGCFLSF
                     VTGIGIFTTIYLTPLFLGYVRGFSAWQTGMAIFSTGAASLIGTPIYILLARKIDVRWL
                     LMGGLASFALAMWSYSFITHDWGTDELLLPQILRGLPQVFVVAPVVNLALGALPPERL
                     KFASGLFNMMRNLGGAVGIAVSAAIINDRTNFHFLHIAARLTPANGAMERLVQSVAQR
                     YGALPGSPQGGHQAAIEQLWHLAYREASTLAFADAFRAIMLAFIVATLLVPLLRNIAA
                     PAAPSANAH"
BASE COUNT          363 a          570 c          705 g          274 t
ORIGIN      
        1 acggacaccg gcagcccggc atggatcgcc gcctggcggg cgggattcat cttgacgccc
       61 gcctggatga cgttgccgag cgccaccgtg gcgatgtcct ccggccggag gcgcgcgcgg
      121 gcgagcgcgg ccttgatggc ggcggcgccg agatcgggcg ccgcgacgtc cttgaggctg
      181 ccgccaaagg ttccgatcgc cgtgcggacc ggttctgcaa agaccactga gcgtgtcatg
      241 gcttctcctt tccgaggatt tgtcttcggt ttaatgcgcg ttggcagaag gcgccgccgg
      301 cgcggcgatg ttgcgcagca gcggcacgag caaggtggcg acgatgaagg cgagcatgat
      361 cgcccggaag gcatcggcga aagcgagggt cgaggcttcg cggtaggcga gatgccaaag
      421 ctgctcgatg gccgcctgat gcccgccctg ggggctgccg gggagcgcgc cataacgctg
      481 ggccacgctc tgcaccagcc tttccatcgc gccgttcgcg ggggtgagac gcgcggcgat
      541 gtggaggaaa tggaagttgg tccggtcgtt gatgatcgcg gcgctgacgg cgatgccgac
      601 ggcgccgccg aggttgcgca tcatgttgaa gaggccgctc gcgaatttga gccgctcggg
      661 cgggagcgcg ccgagggcaa gattgacgac aggtgccacc acgaagacct gcggcaggcc
      721 gcgcaggatc tggggcagca ggagctcgtc cgtgccccaa tcatgagtga tgaagctgta
      781 gctccacatg gcgagcgcaa aggaggcgag gccgcccatg agaagccagc gcacatcgat
      841 cttgcgcgcc agcaggatgt agatcggcgt gccgatcagc gacgcggctc cggtggagaa
      901 gatcgccatt cccgtctgcc aggcgctgaa gccgcgcaca taaccgagaa acagcggcgt
      961 cagatagatg gtcgtgaaga tgccgatccc ggtcacgaaa gagaggaagc aaccgatggc
     1021 gaagttgcgg tcgcggagcg ccctgaggtc gacgacgggc tctgggcgtg tgaggctgcg
     1081 gacgacgaag agcgtgccgg cgatggcgga aatccaggcg caatcgcgga tcgtcgcgtc
     1141 gctgaaccag ttccagcgcg tcccttcctc gagcacgtat tcgagcgtgc cgagggcgat
     1201 cgccatcagg gcgatgccga cataatccgc gccgcgcagc agcgcgagat cgggtttgtc
     1261 gatgcgcacc agcatgggca ccagcgccgt gatggcgagg cccggcacga ggttgacgta
     1321 gaagagccag cgccaatcga gcgtgtcggt gatccagccg ccgatcacgg ggccaagcgt
     1381 gggcgcgacc gaggcaatcg cgccgatgac ggcggccgaa tagacgcggc gcggtccctg
     1441 gaagaagtgg aaggacgagg tgaagacggt cgggatcatc gaggcgccga gcagcccctg
     1501 gagcgcgcgg aagaggatca tgctctcgat gctccaggcg atgccgcaga gcatgctcgc
     1561 ggccgtgaag ccggcggcgg agatggtgaa gagccagcgc gtcgagaaca cgcgcgtcag
     1621 ccagcccgaa agcgggatca cgatgatctc ggcgatgaga taagcggtct ggacccagct
     1681 gatctcgtcc tgcgccgccg acaaaccgcc gccgatattc tgcagcgacg aggccacgat
     1741 ctgaatgtcg aggagcgcga tgaacatgcc cacgcacatg acgacgaagg gcaggagcga
     1801 gggatgcttc ggttcggcgg tcatggcgcg cctttctcgc cgcgggtctc gacgcgcacg
     1861 atggtcgaga ggccgggcct gagcgtcgcg agcatcgggt cgccaccatc ga
//
LOCUS       DASYNS010000111         4323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4550552, whole genome shotgun sequence.
ACCESSION   DASYNS010000111 DASYNS010000000
VERSION     DASYNS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4550552"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..516
                     /locus_tag="VGP50_03510"
     CDS             214..516
                     /locus_tag="VGP50_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV8026469.1"
                     /translation="MTMSVNLRALANDRRLLILAWLRDPRAHFPPQRDGDLVKDGVCG
                     VLIARKLGVSQPTASEHLRVLTQAGLIRAKRVKGWTFYRRDTVRIRALKRAILAKV"
     gene            complement(562..1188)
                     /locus_tag="VGP50_03515"
     CDS             complement(562..1188)
                     /locus_tag="VGP50_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEV8026470.1"
                     /translation="MAGQSPFASELWRPLLTLVSAAVVLMGSPGPSTMSVTAVGAAFG
                     PRRSLRYASGLVLGTSAVLLAVASGVVTLLLSIPGLAPALGAVSALYMVYLALRIATA
                     PPLSTRGNDAAAPSFAGGFLLAIANPKAYLAIAAVFTSATVASQSLLLDVLAKTAVLA
                     AMIVVIHFAWLFAGASLARFLRDPPRSRIVNLAFGAILLVTAVLALMR"
     gene            1213..2067
                     /locus_tag="VGP50_03520"
     CDS             1213..2067
                     /locus_tag="VGP50_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV8026471.1"
                     /translation="MTRRLSAAGNQRLEGLCDPRALRARIRIAPGCDGIERVEARFSG
                     PAFSPHRHDTYAIGVTLAGVQSFRYRGEQRHCRPGQCHILHPDEVHDGAAGTAEGFAY
                     RIVYIDPSLIRDALGGGPLPFVAQPVLDLTPAQRARLSPAFDLATPVDDLRRADIATA
                     LADLLASASSRGTTAAARLALPALLRVRALIAAQPARRHSLNELERAAGLDRWTLARQ
                     FRTAFGTSPTRFRTLRQLDDVRRLLQHGAPLAAAALAAGFADQTHMSRQFKRAYGLTP
                     ARWAAAAR"
     gene            complement(2103..2633)
                     /locus_tag="VGP50_03525"
     CDS             complement(2103..2633)
                     /locus_tag="VGP50_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026472.1"
                     /translation="MTKRKDEETGQKPRRAAFRPTREQRAFVAAAAGLGIPRRVIRQM
                     LPGAKSGETVSITGRMLTDCFAQELREKGLALVVALAGARLCQRALSGDDRNAQTAQM
                     MVLNARGWKLPAPPEERDEISMAALSREERKELRRLLDKATPGQKRGGEGAAEDEISA
                     CGSPLPRTTAPERDWD"
     gene            2895..3719
                     /locus_tag="VGP50_03530"
     CDS             2895..3719
                     /locus_tag="VGP50_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008532075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8026473.1"
                     /translation="MGKITTKDGVEIFYKDWGKGQPIVFSHGWPLSADDWDTQMLFFL
                     NRGYRVIAHDRRGHGRSSQVSDGHDMDHYADDLAALTAHLNLANAIHVGHSTGGGEVA
                     RYLGRHGESRVAKAVLISAVPPLMLKTPANPGGLPKDVFDGLQAQLAANRSQFYRDLP
                     EGPFYGFNRPGAKRSEAIIENWWRQGMMGGAKAHYDGIVAFSQTDFTADLKKITVPVL
                     IMHGDDDQIVPYADSGPLSAKLVKNGTLRTYKGFPHGMPTTQAETINADLLAFLRD"
     gene            3876..>4323
                     /locus_tag="VGP50_03535"
     CDS             3876..>4323
                     /locus_tag="VGP50_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026474.1"
                     /translation="MLSRDQIVQAFTRLGEILWRQKKRGEIAVFRGTAIVLEHDFRNT
                     TKDVDAKITGEHGAVIEAQRQVAAELGLPAYWLNEQATAYLSSKADFALFGAYPDELH
                     PGLLVHVATPEYLLAMKAQSQRIDDIRDAVLLARSTGLTTAEQITNL"
BASE COUNT          738 a         1516 c         1386 g          683 t
ORIGIN      
        1 atgctgcagg agctcgagct tgaggacgag cgtactggac gggaggccga gatcgatgcc
       61 ggcgatctcg atcaccggcg tgcgccggat atgcggccgg atcaccgtct cgatcccggc
      121 gatcgcctcg cgcgtgacat cgctctcgct catcgcccgc tcccgcgtcc cctggtgttt
      181 gacattaaat aagtaaatga gcaaattagc aatatgacga tgtccgtgaa tctgcgcgcg
      241 ctcgccaacg accggcggct gttgatcctg gcatggctgc gcgacccccg cgcgcatttc
      301 ccaccgcagc gcgacggcga tctcgtcaag gacggcgtgt gcggcgtgct catcgcgcgg
      361 aagctcggcg tcagccagcc gacggccagc gagcatctcc gcgtgctgac gcaagcgggc
      421 ctcatccgcg ccaagcgggt caagggctgg accttctacc ggcgcgacac cgtgcgcatc
      481 cgtgcgctca agcgggcgat actggcgaaa gtctgagcgc ggcctgttgc cggcgcgcgt
      541 cgaagcggca gcggcacagg ttcaacgcat cagcgccaaa acggccgtga ccagcaggat
      601 tgccccgaag gcgaggttga cgatgcgcga tcgcggggga tcgcgcagaa aacgcgccag
      661 cgaagcgccc gcgaaaagcc aggcgaagtg gatgacgacg atcatcgccg ccagcacggc
      721 ggttttcgcc aacacatcga gaaggagcga ttggctggcg acggtcgcgc ttgtgaacac
      781 ggcggcgatc gccagatagg ctttcggatt cgcgatcgcg agcagaaagc cgccggcgaa
      841 ggatggagcg gcggcatcgt tgccgcgcgt cgacaacggc ggcgccgtcg cgatcctcag
      901 tgccagataa accatgtaga gggcggagac ggcgccgagc gccggcgcga ggcccggtat
      961 cgacagcagc agcgtgacca cgccgcttgc gaccgcgagg aggacggccg aggtgcccag
     1021 cacgaggccg gacgcatagc gcagcgaccg ccgcggcccg aacgcagcgc cgaccgccgt
     1081 cacgctcatg gtcgagggac cggggctccc catcaggacg acggcagcgg agaccagcgt
     1141 cagcagcggc cgccagagct ccgaggcgaa aggactctgg ccagccatcg ccgtttccct
     1201 aagatcgcaa agatgacgcg aaggctatcc gccgccggca accagcgtct tgaaggcttg
     1261 tgcgatcccc gcgcgcttcg cgcgcgcatc cggatcgcgc cgggatgcga cggcatcgag
     1321 cgcgtcgagg cccgcttttc cggtcccgcc ttttcgccgc atcgtcacga cacctatgcg
     1381 atcggcgtta cgctggcggg cgtgcagagc ttccgctacc gcggcgagca gcgccactgc
     1441 cggcccggcc aatgccatat cctccacccc gacgaggtcc atgacggcgc cgccggcacc
     1501 gccgaaggat ttgcctatcg catcgtctac atcgacccgt cgctcatccg cgacgcgctc
     1561 ggcggcggac cacttccctt cgtcgcccaa cccgtgctcg atctgacgcc cgcgcagcgc
     1621 gcgcgtctct cgcccgcttt cgatctggcg acgcccgtcg acgacctgcg ccgcgccgac
     1681 attgcgacgg ccctcgccga tctgctggcg agcgcttcct cgcgcggcac gacagcggcg
     1741 gcgcgcctgg cgctccctgc cctgctgcgc gttcgcgcgc tcatcgccgc gcagccggcg
     1801 cggcgccact cgttgaacga gctcgagcgc gcggccgggc tcgaccgctg gacgctggcc
     1861 cgccagttcc ggacggcatt cggaaccagc cccacccgct tccgcacgct ccgccagctc
     1921 gacgacgtcc gccgcttgct gcagcacggc gcgcccctgg ccgcggctgc gctcgccgcc
     1981 ggatttgccg atcaaaccca tatgtcgcgg cagttcaagc gcgcctacgg gctcacgccg
     2041 gcaagatggg ccgccgcggc gcgatgacag gacgcgcccg ccgcgccgtc cccgcgcgtg
     2101 attcaatccc aatcccgctc cggcgcggtg gtgcgaggga ggggagaacc acaagccgaa
     2161 atttcatcct ccgccgcccc ttccccgccc cgcttctgcc ccggcgttgc cttgtcgagg
     2221 agccggcgca actccttgcg ctcctcgcgg ctcagggcgg ccatcgaaat ctcgtcccgt
     2281 tcctccggcg gggccggcag cttccagcca cgggcgttga ggaccatcat ctgtgccgtc
     2341 tgcgcattgc ggtcgtcgcc cgacagcgcg cgctggcaga gccgcgcccc ggccagcgcc
     2401 accacgagcg ccagcccttt ctcgcgcagc tcctgcgcga agcaatccgt gagcatacgg
     2461 ccggtgatcg agacggtctc gccggatttt gcgccgggca gcatctggcg gatgacccgg
     2521 cgcgggatcc ccagtccggc cgccgccgcg acgaaggcgc gttgctcccg ggtcggccgg
     2581 aaagcggccc ggcgcggctt ttgtcccgtc tcttcgtctt ttcttttcgt catgatcgct
     2641 ccgcgatgag ccggggctgg gccacgccgc cgccgcggcc ggagcgcccc tccgcctttt
     2701 tcttatgtca ggctcgtcgc gggttaaaga gggtaccgcc ttggtgcctg tggtctcgct
     2761 cgatcatgga gagctgcccc aacgaaggtg tggtcgagac gatacgacgg gacgtcgaag
     2821 ccttcccgag gtgcaagaga aagaagcgga aagcgcggct acccttgggc gcatcatcgg
     2881 gaaaggcgtc aagcatgggc aagatcacaa cgaaggacgg cgtcgagatt ttctacaagg
     2941 actggggcaa gggtcagccc atcgtcttca gccatggctg gccgctctcg gccgacgatt
     3001 gggacacgca gatgctgttc ttcctcaatc gcggctatcg cgtcatcgct catgaccgcc
     3061 gcggccatgg ccgctcctct caggtgagcg acggtcacga catggatcac tatgccgatg
     3121 acctggcggc gctgaccgcg catctcaacc tcgcgaacgc gattcacgtc ggccactcca
     3181 ccggcggcgg cgaggtggcg cgttatctcg gccgtcacgg cgaaagccgc gtcgccaaag
     3241 cggtcctgat cagcgccgtg ccgccgctga tgctgaagac gccggccaat ccaggtggcc
     3301 ttcccaagga cgtcttcgac gggttgcagg cgcagctcgc ggccaaccgc tcgcaattct
     3361 atcgcgacct gccggaggga cctttctatg gcttcaaccg gccgggggcg aagcgctccg
     3421 aggcgatcat cgagaattgg tggcgtcagg gcatgatggg cggcgccaag gcccattacg
     3481 acggcatcgt cgccttctcg cagacggatt tcacggccga cctcaagaag atcaccgttc
     3541 ccgtgctgat catgcatggc gacgacgatc agatcgtgcc ctacgccgac tccggcccgc
     3601 tcagcgccaa gctggtgaag aatggcacgt tgcgcaccta caagggcttc ccgcacggca
     3661 tgccgacgac gcaggccgag acgatcaacg ccgacctcct ggctttcctc agggactgac
     3721 acaagccgcc ccgcgctcgc cctctgatcc gcaaaccgat cggaaccggc gacgctcgtc
     3781 gccggccccg ggttccgctt ccgaggcttg gctcgccaag gtaccggcca cgtcgccgtt
     3841 tggcggtacc atccgctctg ctatatttgg tacccatgct cagcagggac cagatcgttc
     3901 aagcgttcac acgccttgga gaaattcttt ggcgccagaa gaagcgcggc gagatcgctg
     3961 tgtttcgtgg cactgcgatc gtcctcgaac acgacttccg caacaccacg aaagacgtgg
     4021 acgcgaaaat caccggagag catggtgccg tgatagaagc gcagcgacag gtggcagcgg
     4081 agctcggcct gccggcatac tggctcaacg agcaggcgac ggcatatctg agttcgaaag
     4141 ccgatttcgc gctcttcggc gcctatcccg acgagctcca tcctgggttg ctcgtgcacg
     4201 ttgcgacgcc ggaatacctg ctggcaatga aagcacagtc gcagcgtatc gacgacatcc
     4261 gcgacgccgt gcttctcgcc cgctcgacgg ggctgacgac ggcggaacag atcaccaacc
     4321 ttg
//
LOCUS       DASYNS010000112         3383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4238224, whole genome shotgun sequence.
ACCESSION   DASYNS010000112 DASYNS010000000
VERSION     DASYNS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4238224"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VGP50_03540"
     CDS             complement(<1..224)
                     /locus_tag="VGP50_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266395.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEV8026475.1"
                     /translation="MQDAYVYDGLRTPIGRHAGGLAPVRPDDLLSGVIKAVVARNPFK
                     PGDYEDAVVGCTNQAGEDARNVARHAALLAG"
     gene            437..1399
                     /gene="argC"
                     /locus_tag="VGP50_03545"
     CDS             437..1399
                     /gene="argC"
                     /locus_tag="VGP50_03545"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEV8026476.1"
                     /translation="MVKRIFVDGEAGTTGLQIRQRLTRHGGVALISLPEQQRKDTAAR
                     AEAMRGADLVILCLPDDAAREAASLAKGDTRVIDASSAHRTHPDWTYGFPEWKSGHAA
                     RIREARLVTNPGCYAIASVALLSPLVAERLVPADHPVTINAVSGYSGGGKALIDRYEN
                     PKNERHIAGAFRAYALDLEHKHGGEIGKWSGLARPPLFVPGTARYRQGMLVQIPLPLW
                     SLPKPVSASQVRECLAAHYAGAAFVRVGPLHLSDRSAELDPETRNGTNELELLVFANE
                     ARGQVVLCAVLDNLGKGAAGQAVQNLNLMLGLDPATALLERLPP"
     gene            1524..2207
                     /locus_tag="VGP50_03550"
     CDS             1524..2207
                     /locus_tag="VGP50_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEV8026477.1"
                     /translation="MGIFYDQASKLVRNIYDRRIAGPPILDAPTYFPHADRFGGAWHE
                     IREEADRVATRLHTVPRFHDIMREQADISANDGRDWRLFVLKAYGIDSPEHMAACPRL
                     ARIVAATPGVLSTSISFLEPGKHIPPHRGPFRGVLRFYLGLSVPRHANGSPAVVLKID
                     GKEHRVGDGEWLIWDDTYTHEVLNEGDTWRSVLLLDLWRREMPYDMDLLSRLVIGVIR
                     ANVRWRGVR"
     gene            2321..2992
                     /locus_tag="VGP50_03555"
     CDS             2321..2992
                     /locus_tag="VGP50_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEV8026478.1"
                     /translation="MKLYMHPASTTSRAVMLFIADNKIPCEQQVVDIFTGEHTRDPFA
                     TINPSKLIPVLEDGEFRLTESAAILRYIAEKTGSPTYPKDLKQRARVNEVMDWFNSQF
                     YRDYGYGLIYPQLFPNLKRPSKEVQDATVAWGKDFTKRWLQILNDHILGKKKFLCGDE
                     ITIADYFGAAIIQVGEVIRVDLQAYPNIARWLATMKHRPAYNKVYEAVHGFAESVKNQ
                     PFVAL"
BASE COUNT          549 a         1170 c         1093 g          571 t
ORIGIN      
        1 cccgccagca gcgccgcgtg gcgcgccacg ttgcgcgcgt cctcgcccgc ctggttggtg
       61 cagccgacca cggcgtcctc gtagtcgccg ggcttgaacg gattgcgcgc caccacggcc
      121 ttgatgacgc cgctcagaag atcgtcgggc cgcaccggcg ccagcccgcc ggcatggcgg
      181 ccgatcggcg tgcgcagccc gtcatagaca taagcgtcct gcatgctcat cccctcgatc
      241 cctcgggccc gcgggagtaa acgccaggcg agccttctat ccattgcgtg cgagacgggc
      301 agcctaggga aaccgcgacg gagcgacaag ccgtggctgg ggcatggctg ccttgcccgg
      361 cattgtgctc ggcggcgtgc ttatcttagg atcgcgccgc ggctgcatgg cgctgcggct
      421 ggtccggagg accattatgg tgaagcgcat cttcgtcgat ggcgaggcag ggacgacggg
      481 gctgcagata cgtcagcgcc tcacgcgcca tggcggcgtc gcgctcatct cgcttcccga
      541 gcagcagcgc aaggacacgg ccgcgcgtgc cgaggcgatg agaggcgccg atctcgtgat
      601 cctctgcctt cccgacgacg ccgcgcgcga agcggcgagc ctcgccaagg gcgacacgcg
      661 ggtgatcgat gccagctccg ctcatcgcac gcatcccgac tggacctatg gctttcccga
      721 gtggaaatcg ggacacgccg cgcggatccg cgaggcgcgg ctcgtcacca atcccggatg
      781 ctatgccatc gcctcggtgg cgctgctctc gccgctggtc gcggagcggc tcgtgccggc
      841 cgatcatccc gtcacgatca acgccgtctc gggctacagc ggcgggggca aggcgctgat
      901 cgaccgctac gagaacccga aaaacgaaag gcacatcgcc ggcgccttcc gcgcctacgc
      961 gctcgacctc gagcacaagc acggcggcga gatcgggaag tggagcgggc tcgcgcgccc
     1021 gccgctcttc gtccccggca cggcgcgcta tcgccagggc atgctggtcc agatcccgct
     1081 gcccctctgg agcctgccga agccggtgag cgcgagccag gtccgggaat gcctcgccgc
     1141 ccactatgcg ggcgccgcct tcgtccgggt cgggccgctg cacctctccg accggtctgc
     1201 cgagctcgat cccgagaccc gcaacggcac caacgagctc gagctactcg tctttgccaa
     1261 cgaggcccgc ggccaggtcg tgctctgcgc cgtcctcgac aatctcggca aaggcgccgc
     1321 cggccaggcg gtccagaacc tcaatctcat gctgggcctg gatccggcga cggcgctgct
     1381 ggagcgcctg ccgccctaag tcccaaacgc cacgatcgtt gccgggggca gcggcgtcgc
     1441 caggcccttg gatatcctgg atgtccggcc gcgtatatat tatcaaccgc cgggggggcc
     1501 cggcgccggg gcggatgagg gacatgggga tcttctacga tcaggcgtcg aagctcgtgc
     1561 ggaacatcta cgaccgccgc atcgccggtc ccccgatcct cgacgcgccg acctatttcc
     1621 ctcatgccga tcgctttggc ggcgcctggc acgagatccg cgaggaggcc gaccgcgtcg
     1681 ccacccgcct ccataccgtg ccccgcttcc acgacatcat gcgcgagcag gccgatatct
     1741 cggccaatga cggccgcgac tggcggctct tcgtgctgaa ggcctatggc atcgattcgc
     1801 cggagcatat ggcggcctgt ccgcgcctcg cccggatcgt ggcggccacc cccggcgtgc
     1861 tctcgacctc gatctccttc ctggagccgg gcaagcacat tccgccccat cgcggccctt
     1921 ttcgcggcgt gctccgcttc tatctcggcc tctccgtgcc gcggcacgcc aatggcagcc
     1981 cggccgtggt gctgaagatc gacggcaagg agcaccgggt cggcgacggc gaatggctga
     2041 tctgggacga cacctatacg cacgaagtgc tgaacgaggg cgacacctgg cgcagcgtgc
     2101 tcctgctcga tctgtggcgc cgcgagatgc cctatgacat ggacctcctg tcgcggctgg
     2161 tgatcggcgt catccgtgcc aatgtgcgct ggcgcggcgt caggtaggtc cggatcggta
     2221 ctatcatcag gcctgacttt cgccggattc tgtggcagaa cggcagcctg caaccggcgg
     2281 cgccgggaaa tgcggaggct atcgggaggg agttttcatt atgaagcttt acatgcatcc
     2341 ggcgtcgacc acgagccggg cggtgatgct gttcatcgcc gacaacaaga tcccctgcga
     2401 gcagcaggtc gtcgacatct tcacgggcga gcacacgcgc gaccccttcg cgacgatcaa
     2461 tccgtcgaag ctcatcccgg tgctggagga tggcgagttc cgcctcaccg agagcgcggc
     2521 gatcctcaga tatatcgccg agaagaccgg ctcgccgacc tatcccaagg acctgaagca
     2581 gcgcgcccgc gtcaacgagg tcatggattg gttcaattca caattctacc gggattacgg
     2641 ctacggcctc atctatccgc agctttttcc caacctgaag cgtccgtcga aggaagtgca
     2701 ggacgcgacc gtcgcctggg gcaaggactt caccaagcgc tggctgcaga tcctcaacga
     2761 ccacattctc ggcaagaaga agttcctttg cggcgacgaa atcaccatcg ccgactattt
     2821 cggcgcggcg atcatccagg tgggcgaggt catccgcgtc gacttgcagg cctatcccaa
     2881 catcgcgcgc tggctcgcga cgatgaagca tcgcccggcc tacaataaag tctatgaggc
     2941 ggtgcacggc tttgccgaat cggtaaagaa ccagcctttc gtcgctctct gagcgctgcg
     3001 tcagtaacga gagcgacgcc cggccgcttg ggagcgcaga gggcgcagag gtcgcgcgga
     3061 ggccgcagag aataattgtt cctcagcgcc ctctgcgtga cctcggcgtc ctccgcgttc
     3121 gcttttactt ctcgcgggct cttgtccttc gccgccgtcg gcgtcatgga tggcgcttcg
     3181 gttcggcatt tcccggtcca ggagcgcgaa gcaggtcatg cgcgattttc atcaaccgcg
     3241 ccgctcggcc gcggtagcgg gcgaagcgat ggtcgcgagc tcgcatccgc tggcggcgct
     3301 gaccgcgctc gaggtgctgc gcgccggcgg caatgccgtc gacgccgcga tcgctgcttc
     3361 gggcgtgctg gcgatcgccg agc
//
LOCUS       DASYNS010000113         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1743743, whole genome shotgun sequence.
ACCESSION   DASYNS010000113 DASYNS010000000
VERSION     DASYNS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743743"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="VGP50_03560"
     CDS             <1..1307
                     /locus_tag="VGP50_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV8026479.1"
                     /translation="FTSQAWNMAFSFYQSLRTVPAELVEASRSFRLSPWMNFWRVDVP
                     FAMPQLIWNMMMSMSGSWFFVVASEVISVGNTTVTLPGVGSYIALAIAQRDLAAVGYA
                     IVTMLVVILVYDQVLFRPLVAWADRFRFEQEAGDLPPRSWMLDVLRRSRLVDRLTAPF
                     AMLWARSMRARWFDRGGAYKQAARAEGGWAPLVWNGVILALGAVALWQVVRFATTGVS
                     LGDVGTTFLLGLVTLCRVVVLIAIASCIWVPVGVWVGTRPRAANLVQPVAQFLAAFPA
                     NLLFPIVVSAIVVFKLSPNIWLSPLMILGTQWYILFNVIAGAAAIPAELRAVGTNFRV
                     NGWLWWRRIALPAVLPYYVTGAITASGGSWNAAIVAEVASWGDQKLEAVGLGAYIAKE
                     TEAGDFHRIALGIAVMSLFVVLVNRTFWRPLYRYAERKFRLG"
     gene            1328..2611
                     /locus_tag="VGP50_03565"
     CDS             1328..2611
                     /locus_tag="VGP50_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV8026480.1"
                     /translation="MSAALLDVHALRQTFPRADGGELLVLDGIELQLAQGQIVGLLGR
                     TGSGKSTLLRLIAGLVPPSGGAVTYLGEKVVGPAPGIAMVFQSFALFPWLTVLENVQL
                     GLEALGLPEAEIRQRALAAIDLIGLDGYESAYPRELSGGMRQRVGFARALVVHPNILL
                     MDEPFSALDVLTAETLRTDFLELWGEGKLPIKGVIIVTHNIEEAVLMCDRILLFSTNP
                     GRIIREIAVDLKQPRNRLDPQFRDLVEKIYVAMTARAQRSARTVAMSIDAILPRVSAN
                     TISGLLETLAGEPYKGKADLPVIADESSLEVDELFAIGDALQMLHLAEVEGGDIRLTE
                     IGKQFVEAGTDDRKKIFQRQLLAYVPLAAHIRRILQERANHVAPKSRFFDELEDHMST
                     DDAEETLRSVTAWARYAEAFAYDDDSQTFSLENPT"
BASE COUNT          393 a          887 c          860 g          495 t
ORIGIN      
        1 tcttcaccag ccaagcctgg aacatggcct tcagcttcta tcaatcgctg cgcacggtgc
       61 cggccgaact ggtcgaggcg tcgcgcagtt tccggctgag cccgtggatg aacttctggc
      121 gcgtcgatgt gccgttcgcg atgccgcagc tcatctggaa catgatgatg tcgatgtcgg
      181 gaagctggtt cttcgtcgtc gcctccgagg tgatcagcgt cggcaacacg acggtgacgc
      241 tgccgggcgt cggctcctac atcgcgctcg ccatcgcgca gcgggatctg gcggcggtcg
      301 gctacgcgat agtcaccatg ctggtcgtca tcctcgtcta cgaccaggtg ctgttccggc
      361 cgctggtggc gtgggccgac cgtttccgct tcgagcagga ggccggcgac ctgccgccgc
      421 gctcgtggat gctggacgtc ctgcgccgct cgcggctggt cgaccgcctg accgcgccgt
      481 tcgccatgct gtgggcgcgg tcgatgcggg cacgctggtt cgaccgcggc ggagcgtaca
      541 agcaggccgc gcgcgctgaa ggcggctggg cgcccctcgt ttggaacggc gtcattcttg
      601 cgctcggcgc cgtggcgctt tggcaggtcg tgcgctttgc caccacgggc gtgagcctcg
      661 gcgacgtcgg cacgacgttc ctgctcggcc tcgtcacctt gtgccgcgtc gtcgtgctga
      721 tcgccatcgc cagctgcatc tgggtgccgg tcggcgtctg ggtcggcacg cggccgcgcg
      781 cggccaatct ggtgcagccg gtggcgcaat tcctcgccgc ctttccggcg aaccttctgt
      841 tcccgatcgt ggtctcggcc atcgtcgtgt tcaagctcag tcccaacatc tggctgagcc
      901 cgctgatgat cctcggcacg caatggtaca ttctctttaa cgtcattgcc ggcgccgcgg
      961 cgataccggc ggagctgcgc gccgttggca ccaatttccg cgtcaacggc tggctgtggt
     1021 ggcggcgcat cgcgctgccg gcggtgctgc cgtactatgt gaccggcgcg atcaccgcct
     1081 cgggcggttc gtggaatgcg gcgatcgtcg ccgaggtcgc ttcctggggc gaccagaagc
     1141 tcgaagcggt cgggctcggc gcctatattg ccaaggagac tgaggccggc gatttccatc
     1201 gcatcgcgct cggtatcgcg gtcatgagcc tgttcgtcgt cctggtcaat cgcaccttct
     1261 ggcggccgct ctaccgctac gccgagcgca agttccgcct gggctgaaga gcaaaaggaa
     1321 gaacgccatg agcgccgcgc tgctcgatgt tcatgctctg cgccagactt ttccgcgggc
     1381 cgacggcggc gagcttttgg tgctcgacgg catcgagctg cagctcgcgc aggggcagat
     1441 cgtcgggctc cttggccgca ccgggtcggg caagtcgaca ctgctgcgcc tgatcgcggg
     1501 cctggttccg ccgtcgggag gagccgtcac ctatctcggc gagaaggtgg tcgggccggc
     1561 gcccgggatt gcgatggtgt ttcagtcctt cgcgctcttt ccctggctga cggtgctcga
     1621 aaacgtgcaa ctcggtctcg aggcgctagg gttgccggaa gcagagatcc gccagcgtgc
     1681 gctcgcggcg atcgatctca tcgggctcga cggctacgaa tccgcctatc cgcgcgagct
     1741 ttccggcggc atgcgccagc gcgtcggctt cgcccgcgcg ctcgtcgtcc atcccaacat
     1801 tctgctcatg gacgagccgt tctcggcgct cgacgtgctc acggcggaaa ccctgcgcac
     1861 cgacttcctc gagctgtggg gcgagggcaa gctgccgatc aagggcgtca tcatcgtgac
     1921 ccacaacatc gaggaagcgg tgctgatgtg cgaccgcatc cttctgttct cgaccaatcc
     1981 cggacgcatc attcgcgaga tcgcggtcga tctcaagcag ccgcgcaacc gcctcgatcc
     2041 gcaattccgc gacctggtcg agaaaatcta cgtggcgatg accgcgcgcg cgcaacgctc
     2101 ggcgcggacg gtggcgatga gcatcgacgc cattctgccg cgcgtgtccg ccaacaccat
     2161 ctccggtctc ctcgaaacgc tggccggcga gccgtacaag ggcaaggccg atctcccggt
     2221 gatcgccgac gaatcgtccc tggaggttga cgagctgttc gcgatcggcg acgccttgca
     2281 gatgctccac ctcgccgagg tcgaaggcgg cgacatcagg ctcaccgaga tcggcaagca
     2341 gttcgtcgag gcgggaaccg acgaccgcaa gaagatcttc cagcgccagc tcctggccta
     2401 cgtcccgctc gccgcccata tccgccgcat tctgcaggag cgcgcgaacc acgtggcgcc
     2461 gaagagccgc ttcttcgacg agctcgaaga tcatatgagc accgacgacg ccgaagagac
     2521 gctgcgctcg gtcactgcct gggcccgcta cgccgaagcc tttgcctacg acgacgattc
     2581 gcagactttc agtctggaaa atccgacctg aaccgtcagg ccgcgctgcg ccgcg
//
LOCUS       DASYNS010000114         4455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3614974, whole genome shotgun sequence.
ACCESSION   DASYNS010000114 DASYNS010000000
VERSION     DASYNS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3614974"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VGP50_03570"
     CDS             <1..288
                     /locus_tag="VGP50_03570"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEV8026481.1"
                     /translation="ERSTAAVDALVMPTTALVAPPIAAFDKDEDYWRLNSRLLRNTAI
                     INFLDGCAVSLPIHPPGTAPVGLMIAARNGQDRRLLGIALGIEATFGRARG"
     gene            complement(320..685)
                     /locus_tag="VGP50_03575"
     CDS             complement(320..685)
                     /locus_tag="VGP50_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEV8026482.1"
                     /translation="MAAVLRLSEYRRRHRTVFFSRPELNRLLSLYSRQVARGEWRDYA
                     IGQRDGVALFSVFRHTQEAALYTVVKTAPEGGKQNAFALLRGRQVLASGPGLGEVLKK
                     LRPAVLLDGVFGAHSTPSG"
     gene            952..1641
                     /locus_tag="VGP50_03580"
     CDS             952..1641
                     /locus_tag="VGP50_03580"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8026483.1"
                     /translation="MSDIFQEIDEELRRENFAKLWQRYGIYVIALAVVVVLATGGVVA
                     WRQYQSKQRQAEGARYTLALDLARQGKDKDAAEVFGEITRQAGAGRAMLARFETAALK
                     AKSGDVKDAIGQYQALAADTAIDPVYRGLATLLWAQYQLRDGDPKAIIEALVPLTGYE
                     NPWHPSALELTALAQIKAGNKEEALAIYQRLAEDPGAPRGARARAAEIAGTRAASAPV
                     PEITVERRLGQ"
     gene            1638..2972
                     /locus_tag="VGP50_03585"
     CDS             1638..2972
                     /locus_tag="VGP50_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV8026484.1"
                     /translation="MTRGFGRAAALLLVLTLAGCDWFSSIFDESSGKKPLPGDRLSVL
                     GLERRLEPDPALADAEIRLPDAVLNADWPQAGGRSNHAMQHVQLDGTLHQQWTTSVGD
                     GASRYGEPLSQPIVAGGRVFAMDSQDVVVAIDAATGHRVWENDLEPEAAKDHAFGGGL
                     AVAGDRVFVTTGYGQILALEVATGHQIWRQQVASPMRGAPTVADNRVFAITVENRLEV
                     LSAEDGHRLWTHSGLPETAGLVGAASPAVEGDIVVVPYSSGEIFALRVENGRPLWNDN
                     LATARPLGALSTLADIRGRPVIDRGRVFAVSHSGRMVAIDLRSGERVWEQEIGGTHEP
                     WVAGDYVYVLGNDVDLFCLMRQDGRVRWVHELPRYEDEKKKKDPIQWAGPILAGDRLI
                     VVGSNGDGFSLSPYDGKPLGHTSFSDPVYIEPVVAGDTLYVLTDDADLVALR"
     gene            2985..4334
                     /gene="der"
                     /locus_tag="VGP50_03590"
     CDS             2985..4334
                     /gene="der"
                     /locus_tag="VGP50_03590"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV8026485.1"
                     /translation="MALTIAIIGRPNVGKSTLFNRLVGKRLALVDDTPGVTRDRREGE
                     ASLGDLRFGVIDTAGLDEAEEGSLSARMQAQTERALDEADVALFLVDARSGVTPLDRH
                     FADLLRRRKTPVVLAANKAEGRGGAAGLYEAFALGLGEPIAISAEHGDGMVDLYEALA
                     PFAAPPEAETAVTPNGEKPLQLAVIGRPNVGKSTLINRLLGEERVLTGPEPGITRDAI
                     ATLWQWRDRTVRLIDTAGLRRRPKVEEKLEKLSVADTLHAVRFAEVVVLVIDATQLLE
                     KQDLTIARMVEEEGRALVLAVNKWDLVEDKQATLGRLRDRLEISLPQLAGLRFVTLSA
                     RTAAGVAKLMPAVMTTYDAWNRRIATPKLNRWLAAIQQRHPPPLVEGRRLKLRYMTQA
                     NIRPPTFALFASSPGALPDSYRRYLVNLMRADFDLPGVPIRVMLRKGENPYAQAERR"
BASE COUNT          713 a         1495 c         1542 g          705 t
ORIGIN      
        1 gagaggagca cggccgccgt cgacgcgctc gtcatgccga ccacggcgct ggtcgcgccg
       61 ccgatcgccg ccttcgacaa ggacgaggac tactggcggc tcaattctcg tctgctgcgc
      121 aacaccgcga tcatcaactt cctcgacggc tgcgccgtga gcctgccgat ccaccctccg
      181 ggaacggcgc ccgtcggcct catgatcgcc gcgcgaaacg gccaggaccg gcggctgctg
      241 ggcatcgcgc tcggcatcga ggcgacgttc ggccgcgccc gcggctgacg gcggccctga
      301 tctcgcagag cggcagcggt taaccgctgg gcgtgctgtg agcgccgaag acgccgtcga
      361 gaagcaccgc cggccggagc ttcttgagca cctcgccgag gccggggccg ctggccagga
      421 cctggcgccc gcggagcagg gcgaaggcgt tctgctttcc gccctccggt gccgtcttga
      481 cgaccgtgta gagcgccgcc tcctgggtat ggcgaaagac cgagaacagc gcgacaccat
      541 cgcgctgacc gatcgcatag tcgcgccatt cgccgcgggc gacctgacga ctatagaggc
      601 tcaacaggcg gttgagctcg ggccggctga aaaagacggt cctgtgccgt cgccgatact
      661 cgctgagccg caggacggcc gccatcggcc gtgtctcttg gttggtgctc attcgttcga
      721 tgcggaaata agtcttcctc atttccggcc gcaggtctag cccggatttc ccggatttcc
      781 cgcacgcgca cgctgtgcgc cgcgcccgtg ttggcgcgat gtcagaagcg aagcattttg
      841 gatgccggac ccccgaagcc cgtcacgatg cggcggaaat ccgggcgagc agtttgccta
      901 ggaggggtcc agtcgctatg gtagcggccg cgtgcgcgcc cggagtgttt cgtgagtgat
      961 attttccagg aaatcgacga ggaactgcgg cgcgagaact tcgccaagct gtggcagcgc
     1021 tacggcatct atgtgattgc gctggcggtg gtcgtcgtgc tcgccaccgg gggagtggtg
     1081 gcgtggcgcc aatatcagtc gaagcaacgg caggctgagg gggcgcgcta cacgctggcg
     1141 ctcgatctgg cgcgccaggg caaagacaag gatgccgccg aagtcttcgg cgagatcacc
     1201 cgtcaggcgg gtgcgggacg ggccatgctc gcgcggttcg agacggcggc cctcaaggca
     1261 aagtccggcg acgtcaagga cgcgatcggg caataccagg cgctggccgc cgacaccgcg
     1321 atcgaccccg tctaccgcgg cttggcgacg ctgctctggg cgcagtatca gctcagggac
     1381 ggcgatccca aggcgatcat cgaagccctg gtgccgctga ccggctacga gaacccctgg
     1441 cacccgagcg cgctcgagct cacggcgctc gcgcagatca aagccggcaa caaggaagag
     1501 gcgctcgcca tctatcagcg cctggccgag gatccggggg cgccgcgcgg cgcccgcgcc
     1561 cgggcggcgg agatcgccgg cactcgggcg gcatccgctc ccgtgcccga gatcaccgtc
     1621 gaacgacgcc tcggccaatg acgcgtgggt tcggtcgcgc cgccgccctg ctgctggtgc
     1681 tgacgcttgc cggctgcgac tggttcagca gcattttcga cgagtccagc ggcaagaagc
     1741 cattgccggg cgaccggctg tcggtgctgg ggctggagcg ccggctcgag ccggatccgg
     1801 cgcttgccga cgccgaaatc cgtctgcccg acgccgtcct gaatgccgac tggccgcaag
     1861 ccggtggtcg ttcgaatcat gccatgcagc atgtgcagct cgacggcacg ctgcaccagc
     1921 aatggacgac cagcgtcgga gatggggcgt cgcgttatgg cgagccgctc tcgcagccga
     1981 tcgtggcggg cggccgggtg ttcgccatgg attcgcagga cgtggtggtc gcgatcgatg
     2041 ctgcgacggg acatcgggtc tgggagaacg acctggagcc cgaggccgcc aaggaccacg
     2101 ccttcggtgg cggtctggcc gtcgccggcg accgggtgtt cgtcaccacc ggctatggtc
     2161 agatcctggc gctcgaggtc gcgaccggtc accagatctg gcgccagcag gtggcgtcgc
     2221 ccatgcgcgg cgcgccgacc gtcgccgaca atcgggtctt tgccatcact gtcgagaatc
     2281 gcctcgaggt gctctccgcc gaagacggcc accgcctgtg gacgcatagc ggtcttcccg
     2341 agacggccgg cctcgtcggt gcggccagcc cggcggtcga aggcgatatt gtcgtggtgc
     2401 cctacagttc aggcgagatc ttcgcgctcc gtgtcgagaa tggccggccg ctctggaacg
     2461 acaatcttgc gaccgcgcgg ccgctcggcg ccctctccac gctggccgac atccgcggcc
     2521 ggccggtcat cgatcgcggc cgcgtcttcg ccgtgagcca tagcggccgc atggtcgcca
     2581 tcgacttgcg gagcggcgag cgggtctggg agcaggagat cggcggcacc catgaacctt
     2641 gggtcgccgg ggactatgtc tatgtattgg gtaacgatgt cgacctcttt tgcctcatgc
     2701 ggcaagacgg acgcgtgcgc tgggtgcatg agctgccgcg ctacgaggac gagaagaaga
     2761 aaaaggaccc gatccaatgg gccgggccga tcctggcggg tgaccggttg atcgtggtcg
     2821 gctcgaatgg cgacggcttc tcgctttcgc cctatgacgg caagccgctc gggcacacca
     2881 gcttctccga tcccgtctac atcgagccgg tcgtggcggg cgacacgctc tatgtgctga
     2941 cggacgacgc cgacctcgtc gcgttacgct gaccggatgc gcccatggcg ctcaccatcg
     3001 ccatcatcgg ccgccccaat gtcggcaagt cgacgctctt caatcggctt gtgggcaaac
     3061 gcctggcgct cgtcgacgac acgccgggcg tgacacgcga ccgccgcgag ggcgaggcgt
     3121 cgctgggcga cctccgcttt ggcgtcatcg acaccgccgg gctcgacgag gccgaggagg
     3181 ggagcctcag cgcgcgcatg caagcgcaga ccgagcgcgc tctcgacgag gccgatgtgg
     3241 cgctcttcct ggtcgacgcg cgaagcggcg tgacgccgct cgatcgccat ttcgccgatc
     3301 tgctgcgccg ccggaaaacc ccggtcgttc tcgccgcgaa caaggcggag ggacggggcg
     3361 gcgcggccgg gctctatgag gccttcgcct tgggtctggg cgagcccatc gcgatctccg
     3421 cggagcatgg cgacggcatg gtcgacctct acgaggcgct ggcgcctttc gccgcgccgc
     3481 ccgaagccga aacagcggtc acgccgaacg gcgagaaacc gctgcagctc gcggtcatcg
     3541 gccggcccaa tgtcggaaaa tcgacgttga tcaaccggct cctgggcgag gagcgggtgc
     3601 tcacgggacc ggagcctggg atcacgcggg acgcgatcgc gacgctctgg caatggcgcg
     3661 accgcaccgt ccggctcatc gataccgcgg gcttgcggcg ccgccccaag gtcgaggaga
     3721 aactcgagaa gctgtcggtc gcggatacgc tgcatgccgt gcgctttgcc gaagtggtcg
     3781 tgctggtgat cgacgcgacg cagctcctgg agaagcagga cctgacgatc gcgcgcatgg
     3841 tcgaggagga gggccgcgcg ctcgtgctcg ccgtcaacaa atgggacctc gtcgaggaca
     3901 agcaggcgac gctcggccgc ctgcgcgacc ggctcgagat ctcgctgccg cagctcgccg
     3961 gcttgcgttt cgttaccctc tccgcgcgca ccgccgccgg cgtggcgaag ctcatgccgg
     4021 cggtcatgac gacctacgat gcctggaacc gccgcatcgc cacgccgaag ctcaaccgct
     4081 ggctcgccgc catacagcag cgccatccgc cgccgctggt cgaggggcgg cgcctcaagc
     4141 tgcgctatat gacgcaggcc aacatccgtc cgccgacctt cgcgctcttt gcgtcgagcc
     4201 ccggcgcctt gccggacagc taccgtcgct atctcgtcaa cctcatgcgt gcggatttcg
     4261 acctgcctgg ggtgccgatc cgcgtcatgc tgaggaaggg ggagaacccc tatgcgcagg
     4321 cggagcggcg gtgacgcgag ctcgagatga gagcgcatct caagcaaagg aacggtccgc
     4381 gcaaccggga tgcgctctag cttcgctggg ccgagatcgc cacttgtgtc aaagaagcgc
     4441 ttggcgcttc cggtc
//
LOCUS       DASYNS010000115         6136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2523436, whole genome shotgun sequence.
ACCESSION   DASYNS010000115 DASYNS010000000
VERSION     DASYNS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2523436"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VGP50_03595"
     CDS             complement(<1..626)
                     /locus_tag="VGP50_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014106089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV8026486.1"
                     /translation="MRAMTLAAAHQPLGLEERPQIPQPGPRDVLIRVHACGVCRTDLH
                     IVDGELTDPKLPLVPGHEIVGRVAAVGPEVGRLREGDRVGVPWLGWTCGQCDYCRSGR
                     ENLCVRARFTGYHIDGGYAEYTVAHEHFCFPLPGDMDDVAAAPLLCAGLIGYRALRLA
                     GDAPRLGLYGFGAAAHIAVQVARHRGQRVFAFTKPGDVAGQDFARGLGA"
     gene            714..1256
                     /locus_tag="VGP50_03600"
     CDS             714..1256
                     /locus_tag="VGP50_03600"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEV8026487.1"
                     /translation="MLDNLARNWWALALRGVAAIVFGLATLLWPGITLWVLVLFFAAY
                     MLVDGIFAIVTGLRAAQYHRRWWPLILEGVADFVAGAVALLWPRLALLGFIYVAAVWA
                     ILSGIALLIAALRLHRAYGEVTLILGGLVSIAWGILVVVWPIAGELALAWWIGAYALL
                     FGVFMLTFALSLRRRPRAGL"
     gene            complement(1274..2263)
                     /locus_tag="VGP50_03605"
     CDS             complement(1274..2263)
                     /locus_tag="VGP50_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEV8026488.1"
                     /translation="MTTSLPSLNGLRAFEAAARHRSFTRAAVELNVTQTAVSHQIRRL
                     EEQLGVRLFTRRNRALQLTPVAESYLPAVRAAFEDLRQATERLRRRDRDELLTVSTIA
                     SLAAKWLLPRVALFQETHPGIEVRLTASTHLVDFRREEVDMAVRYGRGHWPGLRADWL
                     MAEDIFPVCSPALLSGEKPLREPKDLVHHTLLHATLAREDWQLWLTAAGLPTGLAAKR
                     GLTFDQSFMAIQAAIEGHGVALGRTPIVEADIAAGRLVVPFDLVLPAEAGYYVVAPEE
                     TANAPKIALFRDWLLASVANTPHPNPPPQGGRGLRQSPEASPSPLEGEGRGGG"
     gene            2375..2578
                     /locus_tag="VGP50_03610"
     CDS             2375..2578
                     /locus_tag="VGP50_03610"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEV8026489.1"
                     /translation="MADIAHHNLINSQASPGLQRLFATLSLWRRRVRERSQLARLSEH
                     DLRDIGVARGTLYDELRKPFWRP"
     gene            2623..3456
                     /locus_tag="VGP50_03615"
     CDS             2623..3456
                     /locus_tag="VGP50_03615"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEV8026490.1"
                     /translation="MQADTATALDQVEAALNYLKPGIDHPFVYAYEAPPGMPQRNIEF
                     EPRRVAIRNARPIARELSLDKEGFLLRRHATAVADFYDDDAVRRVYYPEVEQLVRETT
                     GAAKVVIFDHTRRNSDTPLGEGDIRAREAAASVHNDYTEKSAPQRVRDLLDPAEAEAR
                     LQRRYLEINVWRSIAGPVAAWPLAVADARSIGPRDLVASERRYPDRVGEIYHVAYNPQ
                     HRWFYFPQMRRDEALFIKCFDSAKDGRARLSVHTAFEDPTTPAGAPPRESIEVRTFAF
                     F"
     gene            3635..4849
                     /locus_tag="VGP50_03620"
     CDS             3635..4849
                     /locus_tag="VGP50_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8026491.1"
                     /translation="MLLALSAAILSLCMGLRQSLGLFMRPMTIDLGITASAFSFALAL
                     QNIVWGASQPLVGMLADRYGARPVLILSALLYAAGLVIMTQFGGTLGLDIGGLLSGIA
                     IAGAGFGVLMAVVSRAVPASQRSQAVGAVAAAGSLGTLGLAPLGQQMINGFGWRAALL
                     VFAAIALSMALLAIFLRERDVVEDAAPAAAESEQTLGQALRAAATHPGYLAMTAAFFA
                     CGFQLVFITTHLPSYLALCGLPVSVGASALGLIGLCNTFGTYGAGLLGARYSQKRLLA
                     LVYLLRTLAIIAYIAVPITAASTLVFGAAMGLLWLSVAPLVSGIIGRMFGLKHFSMLY
                     GLVFFSHQLGSFCGALLGGMTFDLTGSYTLGWLSLIVIGLLAFALQWPMDDRPPSARR
                     RRDEPAAEAALA"
     gene            complement(4856..5632)
                     /locus_tag="VGP50_03625"
     CDS             complement(4856..5632)
                     /locus_tag="VGP50_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEV8026492.1"
                     /translation="MSDIAEKFERINPAPAYLQVAAAIEKEITSGRLRPGEPLGTEAA
                     LVKQFGVNRSTVREGIRMLEQAGLVQRDSSRRLTIGFPHYERLASRMTRALVLHEVTF
                     RELWEAALVLDLGAVEQAALRATPETLAELDANIARTEAAAGDWTAVVELDTQWHGLV
                     FKAAGNRVLQLAREPAGLLNLPATELILERVTAALPRLIDAHHRITDAIRKRDPAEAR
                     LWMKRHLHDWRRGFERIGKDLDRPIDQVYLQSTAANERRR"
     gene            5923..>6136
                     /locus_tag="VGP50_03630"
     CDS             5923..>6136
                     /locus_tag="VGP50_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026493.1"
                     /translation="MIFKSESILRAWRWGAGLVLGTAAACVLAASPVKAAGPVAFTQD
                     GTFRGVATGTMNEFLGIRYAQSPAGAF"
BASE COUNT          903 a         2086 c         2081 g         1066 t
ORIGIN      
        1 gcgcccaggc cccgcgcgaa atcctgtccc gcgacatcgc ccggcttggt gaaggcgaag
       61 acgcgctggc cgcgatggcg cgccacttgc accgcgatgt gggcggcggc gccgaagccg
      121 taaaggccga gacgcggcgc atcgccggcc agcctgagcg cgcgatagcc gatgagcccg
      181 gcgcacagca gcggcgccgc tgcgacgtcg tccatgtcgc cgggcaaggg aaagcagaaa
      241 tgctcatgcg ccaccgtgta ttcggcatag ccgccatcga tgtgatagcc ggtgaagcgg
      301 gcgcgcacgc agaggttctc gcggccgctc cggcaatagt cgcattggcc gcaggtccag
      361 ccgagccagg gcacgccgac gcggtcgcct tccctaagcc gcccgacctc gggcccgacc
      421 gccgcgacgc ggccgacgat ttcgtggccg ggcaccagcg gcagcttggg atcggtcagc
      481 tcgccatcga cgatgtgcag gtcggtgcgg cagacgccgc aggcatggac gcggatcagc
      541 acatcgcgcg gcccgggttg cgggatctgc ggccgctctt cgagccccag cggttggtgg
      601 gcggcggcga gcgtcattgc gcgcatgcgt cctcgatttc atgccgttga gagcagcctt
      661 gcggctccta tcggctatat agtggctgtt ggcgagccac ggagggggga ctcatgctcg
      721 acaatctcgc acgcaattgg tgggcgctgg cgctgcgcgg cgttgcggcc atcgtcttcg
      781 ggctggcaac gctgctctgg ccgggcatca cgctctgggt gctcgtgctg ttcttcgccg
      841 cctacatgct ggtcgacggc atcttcgcca tcgtcaccgg gttgcgcgcg gcgcagtatc
      901 atcgccgctg gtggccgctc atcctcgaag gcgtggccga cttcgtggcg ggcgccgtcg
      961 cgctgctgtg gccacggctc gcgcttttgg ggttcatcta tgtcgcggcc gtctgggcga
     1021 tcctgagcgg cattgccttg ctcatcgcgg cgctccggct gcatcgcgcc tatggcgagg
     1081 tgacgctcat ccttggcggc ctcgtatcga tcgcctgggg catcctggtc gtcgtctggc
     1141 cgatcgccgg ggagctcgcg cttgcctggt ggatcggcgc ctatgcgctg ctcttcggcg
     1201 tgttcatgct gaccttcgcg ctgtcgctcc ggcggcggcc ccgggccggg ctttaggcag
     1261 aggcgctcgc ggcctacccc ccaccccgac cctccccctc aaggggggag ggggatgctt
     1321 ccgggctctg ccgcaatccc ctcccccctt gagggggagg gttagggtgg ggggtattcg
     1381 cgaccgaggc gaggagccag tcgcgaaaga gcgcgatctt gggcgcgtta gcggtctctt
     1441 ccggtgccac cacgtagtac ccggcctccg ccggaagcac gaggtcgaag ggcacgacga
     1501 ggcgccccgc ggcgatgtcg gcttcgacga tcggcgtccg gcccagcgcc acgccatgcc
     1561 cctcgatcgc cgcctggatg gccatgaagc tttgatcgaa ggtgaggccg cgtttggcgg
     1621 caagacccgt cggcaagccc gcggcggtca gccagagctg ccagtcctcg cgcgcgaggg
     1681 tggcgtggag cagcgtgtga tggacgagat ccttgggctc gcgcagcggc ttctcgccgc
     1741 tgagcagcgc cgggctgcag accgggaaga tgtcctcggc catgagccaa tcggcgcgca
     1801 agcccggcca atggccgcgg ccgtagcgca ccgccatatc gacctcctcg cgccggaaat
     1861 cgaccagatg cgtcgaggcg gtgaggcgca cctcgatgcc gggatgcgtc tcctggaaga
     1921 gggcgacgcg cggcagcagc catttcgccg cgagcgaggc aatcgtgctc accgtcagca
     1981 gctcgtcgcg gtcgcggcgg cgcaggcgct ccgtcgcctg gcgcagatcc tcgaaggcgg
     2041 cgcgcaccgc ggggagatag ctctcggcca ccggcgtgag ctggagcgcc cggttgcgcc
     2101 gcgtgaagag tcgcacgccc agctgctcct cgaggcggcg gatctggtgg ctgacggcgg
     2161 tctgcgtgac gttgagctcg accgcggcgc gggtgaagct gcggtggcgc gccgccgcct
     2221 cgaaggcgcg caagccgttg agcgagggca ggctggtcgt catgggacgc ggctgaccat
     2281 gagattatat catgcaaggg ctgacaaagt gtcgtttgcg gcaccccgcc gggaggcgca
     2341 tcttacggaa aattcgtatc cctggagagc cgccatggcc gatatcgccc atcataacct
     2401 gataaattct caggcgtcac cgggtctgca gcggcttttc gcgacgctca gcctgtggcg
     2461 ccgccgggtc cgcgagagat cgcagctcgc ccgcttgagc gagcacgatt tgcgtgacat
     2521 cggcgtcgcg cgcggtaccc tttacgatga gctgcgcaag ccgttctggc ggccgtagac
     2581 tcgcgacaac ccaagcgcaa gaagcggagg aaaggaatcg ccatgcaagc cgacacggca
     2641 acggctctgg atcaggtcga ggcggcgctc aactacctga agcccggcat cgaccacccc
     2701 ttcgtctatg cctatgaggc gccgccgggg atgccgcagc gcaatatcga attcgagccg
     2761 cgccgcgttg ccatccgcaa cgcgcggccg atcgcgcgcg agctctccct cgacaaggag
     2821 ggcttcctct tgcgccgcca cgcgacggca gtggccgact tctatgacga cgatgcggtg
     2881 cgccgcgtct actaccccga ggtcgagcag ctcgtccgcg agacgaccgg cgccgcgaag
     2941 gtcgtgattt tcgaccacac gcggcgcaac agcgacacgc ctttgggcga gggcgacatc
     3001 cgcgcccgcg aggctgcggc ctcggtgcat aatgactaca ccgagaaatc ggcgccgcag
     3061 cgcgtgcgcg acctgctcga tccggccgag gcggaggcgc ggctccagcg gcgctatctc
     3121 gagatcaacg tctggcgctc gatcgccggg ccggtggccg cctggccgct cgccgtcgcc
     3181 gatgcgcgca gcattggccc gcgggatctc gtggcgtcgg agcggcgcta tcccgaccgc
     3241 gtcggcgaga tctatcacgt cgcctacaac ccgcagcatc gctggttcta cttcccgcag
     3301 atgcgccggg acgaagcgct tttcatcaaa tgcttcgatt cggccaagga cggccgcgcc
     3361 cggctgtccg tccacaccgc cttcgaggat ccgacgacgc cggccggcgc gccgccgcgc
     3421 gagagcatcg aggtcagaac cttcgccttc ttctgagcga cgtcggtcgc tcccgccatc
     3481 gtcgcaacac agccctgtgc aattgacggt cgcggcgctg gcggtcttcc ggcagcttaa
     3541 gcccagacgt tatcgagatc gatccggagg acgagctttg tccctgaccg ccctgtcacc
     3601 gtccgcgccg gcgcgctggc ccgcgcgtac gttggtgctg cttgcgctca gcgcggcgat
     3661 cctgtcgctc tgcatgggcc tgaggcagag cctcggtctc ttcatgcgtc ccatgaccat
     3721 cgacctcggc atcaccgcct cggccttcag cttcgcgctg gcgctccaga acatcgtctg
     3781 gggcgcgagc cagccgctgg tcggcatgct cgccgaccgc tacggcgcga ggcccgttct
     3841 gatcctctcg gcgctccttt acgcggcggg actggtgatc atgacgcagt tcggcggcac
     3901 gctcggactc gacatcggcg gcctgctcag cggcatcgcc attgccggcg ccggcttcgg
     3961 cgtcctcatg gcggtggtgt cgcgggccgt gcccgcttcg cagcgcagcc aggcggtcgg
     4021 cgctgtggct gcggcgggct cgctcggcac gctgggcctc gctcccctgg gacagcagat
     4081 gatcaacggt ttcggctggc gcgcggcgct cctggtcttc gcggcgatcg cgctgtcgat
     4141 ggcgcttctc gcgatcttcc tgcgcgagcg cgacgtcgtt gaagatgctg ccccggccgc
     4201 ggccgagagc gagcaaacgc tggggcaagc cttgcgcgcg gccgcgactc atccgggcta
     4261 tctcgccatg accgccgcct tcttcgcttg cggcttccag ctcgtcttca tcactacgca
     4321 tctgccgtcg tacctggcgc tttgcggctt gcccgtcagc gtcggcgcga gcgccttggg
     4381 gctcatcggc ctttgcaaca ccttcgggac ctacggcgcc gggctgctcg gcgcgcgcta
     4441 cagccagaag cggctcctgg ctctcgtcta tctgctccgc acgctggcga tcatcgccta
     4501 tatcgcagtg ccgatcacgg cggcgtcgac gctggtgttc ggtgcggcca tgggactcct
     4561 ctggctcagc gtcgccccgc tggtcagcgg catcatcggc agaatgttcg gcttgaagca
     4621 tttcagcatg ctctatggcc tggtcttctt cagccatcag ctcggctcgt tctgtggcgc
     4681 gctgctcggc gggatgacct tcgacctgac gggctcctat acgctcggct ggttgtcgct
     4741 gatcgtcatc ggcctgctcg ccttcgcgct gcaatggccg atggacgacc gcccgccatc
     4801 ggcgcgccgg cgccgcgacg agccggctgc cgaggcagcg ttggcgtaaa gggggctagc
     4861 gccgccgctc gttcgcggcg gtcgattgca gatagacctg atcgatgggc cgatcgaggt
     4921 ccttgccgat ccgctcgaag ccgcggcgcc aatcgtgcag atgacgcttc atccacaagc
     4981 gcgcctccgc ggggtcgcgc ttgcggatcg cgtcggtgat gcgatggtgc gcgtcgatga
     5041 gccgcggcag cgccgcggtg acgcgctcga ggatcagctc cgtcgccggc aggttgagca
     5101 gccccgccgg ctctcgtgcc agctgcagca cgcgattgcc ggcggctttg aacacgagtc
     5161 cgtgccattg ggtatcgagc tcgacgacgg ccgtccaatc gccggcggcg gcctcggtcc
     5221 tggcgatgtt tgcgtcgagc tcggcgaggg tctcgggcgt cgcccgcagc gccgcttgct
     5281 cgaccgcgcc cagatcgagc accagcgcgg cctcccagag ctcgcggaac gtcacctcat
     5341 gcagcaccag cgcccgcgtc atgcgcgagg cgaggcgctc gtaatgggga aagccaatgg
     5401 tcagccggcg gcttgaatcg cgctgtacga ggccggcctg ctcgagcatc cgtatccctt
     5461 cgcggacggt cgagcggttc accccgaact gcttcaccag cgccgcttcc gtgcccagcg
     5521 gctcgccggg acgcaaccgg ccgctggtga tctccttctc gatggccgcg gcgacctgca
     5581 gatacgccgg ggccggattg atgcgctcga atttctcggc gatgtccgac attgcacccg
     5641 tgctcgctgg cgtcagcgta gcacaacaca accatgattg atgcacgcac cgcgccaaaa
     5701 agagatcgcc caacgcccaa caagcttcga gaacaattct acagcgactg ttggttaatg
     5761 ctcaccttct tttgcgaaaa cagggattgt ctgacaatcc gctaagtttt ataataatgc
     5821 aaaacgtgca ttgccatgcg aggccacaga aacttctgcg gccggcgatg tgtcgcgcga
     5881 cgccgaatgg tggactgcgg tcgaaacggg aggataaatc acatgatatt caaatcggaa
     5941 tcgattctaa gagcctggcg ctggggagcc gggctggttc tcgggaccgc ggcggcatgc
     6001 gtgctggccg cgagcccggt caaggcggcg gggccggtgg ccttcacgca agacggcact
     6061 ttccgcggcg tcgcgaccgg gaccatgaat gaattcctgg gcattcgcta tgcgcagtcg
     6121 ccggcgggcg ccttcc
//
LOCUS       DASYNS010000116         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2367912, whole genome shotgun sequence.
ACCESSION   DASYNS010000116 DASYNS010000000
VERSION     DASYNS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367912"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VGP50_03635"
     CDS             complement(<1..591)
                     /locus_tag="VGP50_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8026494.1"
                     /translation="MAFNPNEVNLDPEAKALLDLVAKAGRIPFSQLTPPTARAQFAEL
                     CRRTRKPCDWDVAASDHAIPGPGGSRNARRYLPRRAGPAPLPALLYFHGGGWCIGDVE
                     THDALCRQIAHEAQCAVLSLDYRLAPEHPFPAAVEDCFAALCFAAAEGRSLGLDPARL
                     AVGGDSAGGNLAAVVALLAREAGIAVAGQVLFYPATD"
     gene            677..>1707
                     /gene="rlmN"
                     /locus_tag="VGP50_03640"
     CDS             677..>1707
                     /gene="rlmN"
                     /locus_tag="VGP50_03640"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV8026495.1"
                     /translation="MEHALKDMVGLSREEVAAEMAAIGAQPFRARQLWHWIYNRGARD
                     FAAMTTLSKVFRSELAERYVVGRPETARALSSSDGTRKWLLRFADGQEVECVHIPEED
                     RGTLCVSSQVGCTLTCKFCHTGTQRLVRNLSAQEIVGQVMLARDALGEWPSPQDDRQL
                     TNVVLMGMGEPLFNYDNVAKALKIVMDHEGLSLSRRKITLSTSGVVPMIERCGQELGV
                     NLAISLHAVTDELRDTLVPLNKKYPIALLLEACRNYPGSSNARRITFEYVMLRGVNDS
                     PAEARELVRLLEGIPAKVNLIPFNPWPGAPFERSTAAAIKLFSDIVNAAGYSSPVRTP
                     RGEDIMAACG"
BASE COUNT          282 a          585 c          579 g          261 t
ORIGIN      
        1 atcggttgcc ggatagaaga ggacctgccc cgcaaccgcg atccccgctt cgcgcgcgag
       61 cagcgcgacg accgcggcga gattgccgcc cgcgctgtcg ccgccgaccg cgagacgcgc
      121 ggggtcgagc ccgaggctcc gaccctcggc agcggcaaag cacagcgcgg caaagcaatc
      181 ctcgacggcg gcgggaaatg gatgctcagg cgccagacga taatcgagcg acagcacggc
      241 gcattgcgcc tcatgggcga tctgccggca gagcgcgtca tgggtctcga cgtcgccgat
      301 gcaccagccg ccgccatgga aataaaggag cgccgggagc ggcgcagggc cggcccggcg
      361 cggcaggtag cggcgggcat tgcggctccc gcccggcccg ggaatcgcgt ggtcgctcgc
      421 cgccacgtcc cagtcgcagg gctttcgcgt gcgccggcac agttcggcga attgcgcgcg
      481 cgcggtgggc ggcgtgagct ggctgaaggg aatgcgcccg gctttggcga cgagatcgag
      541 aagcgccttg gcctctggat cgagattaac ctcgttcgga ttgaaagcca tgctgcccca
      601 gcggtttgag ctgcccctgg gcggcgggcc cttttccttt gcgccgccgc cgccccgccc
      661 tatatcttgc gccatcatgg agcatgctct caaggacatg gtgggcttga gccgcgagga
      721 agtcgcggcc gagatggcgg ccatcggcgc ccagcccttc cgcgcccgcc agctctggca
      781 ctggatctac aaccgcggcg cgcgcgactt cgcggcgatg acgacgctgt caaaggtgtt
      841 ccgcagcgaa ctcgcggaac gttacgtcgt cggccggccg gagacggcgc gcgcgctcag
      901 ctcgagcgac ggcacgcgca aatggctgtt gcgcttcgcc gacggccagg aagtggaatg
      961 cgttcacatc cccgaggagg atcggggcac gctctgcgtc tcttcccagg tcggctgcac
     1021 cctcacctgc aaattctgcc acacgggcac gcagcgcctc gtgcgcaatc tgagcgcgca
     1081 ggagatcgtc ggccaggtga tgctggcgcg cgacgcgctc ggtgaatggc cctcgcccca
     1141 ggacgaccgc cagctcacca acgtcgtgct catgggcatg ggcgagccgc tcttcaacta
     1201 cgacaatgtg gcgaaggcgc tgaagatcgt catggatcac gagggcctgt cgctctcgcg
     1261 gcgcaagatc acactctcga catcgggcgt ggtgccgatg atcgagcgct gcggccagga
     1321 gctcggcgtc aatctcgcga tctcgctcca tgccgtcacc gacgagctgc gcgacacgct
     1381 ggtgccgctc aacaagaaat acccgatcgc cctgctgctg gaggcctgcc gcaactatcc
     1441 gggatcgagc aatgcgcggc gcatcacctt cgaatatgtg atgctccgtg gcgtgaacga
     1501 cagcccggcc gaggcgcgcg aattggtgcg gctgctcgag ggcatcccgg ccaaggtgaa
     1561 tctgatcccg ttcaatccct ggccgggcgc gcccttcgag cgctcgaccg ccgcggcgat
     1621 caagctcttc tccgacatcg tcaacgcggc cggctattcg tcgccggtgc gcacgccgcg
     1681 cggcgaggac atcatggccg cctgcgg
//
LOCUS       DASYNS010000117        20361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_182811, whole genome shotgun sequence.
ACCESSION   DASYNS010000117 DASYNS010000000
VERSION     DASYNS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 20361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20361
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182811"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VGP50_03645"
     CDS             <1..774
                     /locus_tag="VGP50_03645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEV8026496.1"
                     /translation="SALKVAETFRVLETLYPGRIDLGIGRAPGGDALTARALQHGPGT
                     FGADYFPSQIADLIGFLRDTMPADHPFATVRAQPQGATAPELWLLGSSDQSAAVAAHF
                     GCAFSFAHFITDQGGPEVMAAYRKYFQPSPWLAAPQGSIGIFVLCADSEAEARRLALS
                     RELWRLRLGRGELGPFPSVAEAEAYAYSREDRLRIAVMRRRAVVGAPEQVRDQLLALG
                     ADYGVSEFVVVTICHDTAARRRSYELLAEAFGLVPRAPP"
     gene            complement(781..1596)
                     /gene="pssA"
                     /locus_tag="VGP50_03650"
     CDS             complement(781..1596)
                     /gene="pssA"
                     /locus_tag="VGP50_03650"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEV8026497.1"
                     /translation="MRRPGVRVKPIGSLSLNRMIPNILTLLALCAGMTAMRFALSNKY
                     ESATVAIMLAAVLDGVDGRIARLLKGTSPFGAQLDSLSDFVSFGAAPAVMLYLWTMQD
                     LKSFGWPVVLLFAVCSAMRLARFNAQIGAELPPYAYNFFTGVPAPAAAMLVMIPMFLT
                     FEFGDGLFRSPYLSGIVVAGVAALMVSRVPTFSFKRVHLPIEWWPPVLLLTVGLVAFM
                     TTEPWATLLAVEALYIASIPLSIRSYRRLRRAAEALRSGVPHEEIEAADHPGL"
     gene            complement(1596..2282)
                     /locus_tag="VGP50_03655"
     CDS             complement(1596..2282)
                     /locus_tag="VGP50_03655"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEV8026498.1"
                     /translation="MLRQVLAPIHPDGWRFIAIALVATAVLFWIYDPLGWIGVVGTAW
                     CVYFFRDPWRVTPEREGLVIAPADGLVVSVGPAVPPPELEMGTAPMTRIGIFLNVFDV
                     HVNRVPMTGRVAKRVYHPGKFFNASLDKASEENERMAIRIATSEGPDIAVVQVAGLVA
                     RRIVCTVSEGDALIAGQRFGLIRFGSRTDVYLPPAWTPFAIVGQRVLGGETVVADCRS
                     QEPPRTGRAQ"
     gene            complement(2367..4457)
                     /locus_tag="VGP50_03660"
     CDS             complement(2367..4457)
                     /locus_tag="VGP50_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026499.1"
                     /translation="MTIAGYSIATAPLLPWVAIIAFAGLAILFLAFGLWRRARGLAWR
                     LAAFVVLLVILANPSLVEEERQPQHDVAVVVVDDSPSMRIGERRRFADEALQHVTQRL
                     QQFPDIDLRVVHAGAPDPKAGLIDSGTQLYTALTRALADVPRQRIAGAVMITDGEVHD
                     VPPPERRAFDAPLHVLLTGRPGEADRRLVVKDAPSFGLVGKELQLTLRVEDLPEGAEN
                     GQARLTLRKDGGAPSSRLVPVGRDVPVTITVDHGGPNVIELEVEPGPHELTLDNNRAA
                     LIVNGVRDRLKVLLVSGEPHQGERTWRNILKSDPSVDLIHFTILRPPEKQDGTPIREL
                     SLIAFPIRELFDVKIEEFDLIIFDRYRRRSILPQAYLENIARYVRKGGALLEAAGPTF
                     GTPLSLYRSPLGAVLPAEPTGNVFEEGFLPRVSEIGRRHPVTAGLPGDSPDGTTPPKW
                     GRWFRHVDAEPHHGVTIMTGLNDRPLLVLDRAGDGRVAQLLSDELWLWTRGFEGGGPQ
                     SELLRRVVYWLMKEPDLEENDLRAVVDGNRLIVTRQSLEKDDRPVQVTGPDGKLETLQ
                     LTPDSGGRSTGSMPVAESGLYRVEDGTRTALAAAGALNPIELADVRTTDEKLHPLVEA
                     TGGGTFWVGPGALPDLRRVAPDRASAGHSWMGFRANGDYVVTGVKEVSLLPGFLALLL
                     ALAAFLFAWRREGR"
     gene            complement(4454..7174)
                     /locus_tag="VGP50_03665"
     CDS             complement(4454..7174)
                     /locus_tag="VGP50_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEV8026500.1"
                     /translation="MLALGPLAFAAPWLLWGLLVLPALWWLLRVTPPAPRRVRFPALR
                     LLLGLTPREETPAKTPLWLILMRMALIALVILALAHPLLNPSAQLGGDGPLVLVVDDG
                     WSAAPHWDEHRAMLDRLIDQAEREHRRIVMLGTTTAPGDIAPPPLSVLRPADARAALD
                     HLRPQPWPPNRPASLDRLAQLAVGNDAEIVWLSDGVDGGEAQDFARGLQRFGSLRVVA
                     DEPGNLPRLLAPADMDDKDLTVTVRRADAAGADRLFVRAVGDDSRLLARQEVTLDPAI
                     DHVVAHLAMPSELRNRAAAIQIEGETSAGAVLLLDERWRRRPVGVVARQQGTAAQPLL
                     SGAYYLERALSPFSEVRRGDADTLLKGEIAVLALPDVAPASVAEKAALGKWMENGGVV
                     LRFAGPHLAEQVDDDLLPVALRRGGRTLGGALSWEKPAKLAPFGADSPFAGLEIPDDV
                     TVSRQVLAEPTVDLAAKTWARLTDGTPLVTADKHGKGWLVLVHTTADPEWSNLAISGL
                     FVSMLRRIAALSQGVSGSSDTALPPVETLDGFGRLERAPSSAQTIAAGAFGNTIASPS
                     HPPGFYGTPDARRALNLFGAVKRFAAIGALPPGVAREGYGRSNEIDFRPGLLGAALAL
                     ALIDLLIAYALRGLLPRLPRRAGGATVGLLLLALALGATPAFADDAFTVEATSELHLA
                     YVRTGIAEVDEESRAGLAGLTDVLSHRTAVEAAAPMEVDPETDDLIFFPLLYWPVVEG
                     QQLPSGKAVERLNRYLATGGTIFFDTRDQGESTPFSAAGTQARLRKLAAGLNIPPLVP
                     VPPDHVLTKSFYLMQDFPGRWAGGSVWVETVEDQVNDGVSSIVVGGNDWAGAWATDQR
                     GQPLYPAVPGGEPQREMAYRFGVNLVMYALTGNYKTDQVHVPAILERLGQ"
     gene            complement(7284..8171)
                     /locus_tag="VGP50_03670"
     CDS             complement(7284..8171)
                     /locus_tag="VGP50_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV8026501.1"
                     /translation="MPGPAALQHRAEQLAASLPPLLVAAERVAATVAQGVHGRRRVGQ
                     GETFWQFRQYSPGDPVPRIDWRESAKSQHLYVRETEWEAAQSVWLWRDSSPSMDYAST
                     AALPSKRQRTDILLLALAALLVRGGERVTLLGSGVPPASGRAVLNRLGLTLERQGSGP
                     ELPQFQPLPRHGQLVWIGDLLAPLETIHALVGRFASGGLRGHLLQVLDPAEETLPFMG
                     RVRFEGFEAEDPLLISRVETVRDDYQARLAKHREGLAAIARATGWSFGTHRTDRPPHT
                     ALLALYGALAEPRRRPGRR"
     gene            complement(8171..9184)
                     /locus_tag="VGP50_03675"
     CDS             complement(8171..9184)
                     /locus_tag="VGP50_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV8026502.1"
                     /translation="MSITDNLSTGAGSTDLVAEIDALGKRLALVRERIGRVIFGQREV
                     IDQALITLLAGGHALLIGVPGLAKTRLVETLGTVLGLEDKRIQCTPDLMPADILGSEV
                     LEESESGRRSFRFIPGPVFCQLLMADEINRASPRTQSALLQAMQEGRVSVAGQYHPLP
                     RPFHVLATQNPLEQEGTYPLPEAQLDRFLLQIDVGYPDLDAERQMLIATTGITEEKAT
                     PVLTAGELLAAQRLVRRIPVGESVVEAILKLVRMGRPEDTSLAEAKQHVAWGPGPRAS
                     QALMLACRARALLEGRLAPSVDDVVALAPPVLRHRMALNFAARADGITMSHIIDRLVG
                     PFT"
     gene            9311..9895
                     /locus_tag="VGP50_03680"
     CDS             9311..9895
                     /locus_tag="VGP50_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEV8026503.1"
                     /translation="MTDKPAPSKRLDRGAAMPNAERVEHGKHPRQPIDCGHFDIRIAR
                     DGTWYYRDSPIARMPLVRLFSTVLRRDPDGTFWLVTPVERGRITVEDAPFVALGLTVE
                     GEGRSQSLIFRTNIDDTVAADGAHPLRVVNHPETGEPNPYILVRDGLEARVSRSVFYE
                     LVALGREERVGEETLYGVWSKGEFFPLGRLDGEA"
     gene            9973..10521
                     /locus_tag="VGP50_03685"
     CDS             9973..10521
                     /locus_tag="VGP50_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEV8026504.1"
                     /translation="MRGDHSLVPGGAPPATVLTPAAVLVPLVMRPEELTVLLTQRTPH
                     LAAHAGQISFPGGSVEPVDVDSIDTALRETTEEVGLPRDHVEIVGRLDTYVTSTGFEV
                     TPVVGLVRAPYPMKLDPFEVAEVFEVPLAFIIDPANHQRESREFKGRMRTFFVLPYQN
                     RYIWGATAGMLVNLSEVLGGLA"
     gene            10518..11741
                     /locus_tag="VGP50_03690"
     CDS             10518..11741
                     /locus_tag="VGP50_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV8026505.1"
                     /translation="MSEVDAPVDRLAPQPWMTEPATHAVLAALSDAGGAARFVGGAVR
                     DALLGRPVGDIDIATPLSPEEVTLRLERAGVTVVPTGIAHGTVTAVVPPRHFEITTLR
                     QDVETFGRQARVAFIADWTGDAGRRDFTMNALFLDIDGRVFDPVGGLEDLHAGRVRFV
                     GDPATRIREDVLRLLRFYRFYAHYGRGEADGAARAACTALAPLLPGLSGERVAAETLK
                     LLAAPDPLPTLAMMAEDGVLAVLLPEAQRRDRLATLVPLEPTPDPLRRLGALVAGDVA
                     QLAERLRLSNEQRDRLTALAQPPWPIDLAGDDAVQRRAVYHLGVERYRDLVLLRAAET
                     GEIERVAALLALAESACPPPFPLKGRDVTALGVPPGPEVGRLLAAVEAWWEEGDFRAD
                     RAACLAEVARRVNER"
     gene            complement(11762..12754)
                     /locus_tag="VGP50_03695"
     CDS             complement(11762..12754)
                     /locus_tag="VGP50_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026506.1"
                     /translation="MPLRSCMVQDGLFERGNGMMILARQQSDGQLAVAGFLLDVFCLG
                     VKDVFFRPFEPSELDEYMDIMGQDVPLSDVDPAYARKLLRDTVAYARSLGLEPHPDFA
                     GVEPVFGEISAEACDVSFEFGLDGKPFYIPGPHESAKQIDRRLRHLQERLGDDGFDVA
                     LDDDDEDDADDDDILDDELELPEGAAPYDPEVAPNPKEWLARSEDERRLVIEAYHRGA
                     GIRVPNQMLHAIMHVAVENQIALGDEVPVRRAIDRLMADGLDRHEALHAAGTVLFEHL
                     TQLMHDVGAGAGAGAGTAESVSHEAYYAALEQLTADGWRRSLEEDEDEDPDEEA"
     gene            complement(12968..13378)
                     /locus_tag="VGP50_03700"
     CDS             complement(12968..13378)
                     /locus_tag="VGP50_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026507.1"
                     /translation="MPAPVYSSDVLAQARRRRLRRVWVALVLGLVVAAYTDGALSQRV
                     DDSNDLLPPWLEAVLLGAVFALASLPVASAVLARAAIPLPTFFLYLSALLGRNPPLPF
                     SAAFGIAFAYAAALTALSAYLSQRPERSLLGSRH"
     gene            complement(13388..15211)
                     /locus_tag="VGP50_03705"
     CDS             complement(13388..15211)
                     /locus_tag="VGP50_03705"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV8026508.1"
                     /translation="MSSATPTRPWHSVRLRARLWLGRVAQRNFRDNEIALLAISALLG
                     AVAGIAVGAIEQFVQLFHAINFAIPFQAHVSAGGAIAWWRLLIVPVVGGLLSGSAAML
                     IRRRRPRETVDAIEANALFGGRMSLTDSALLTGLTIISSGFGASVGMEAGYTQLGAGF
                     ASHVGQRLRMRRGDLRTLVGCGAAAAIAAAFDAPLAGAFYAFELVIGGYSAPVLAPIA
                     VAALSATLVVRGIFGADSIFALDTPVHVSTLDYALFAGLGVVAAAIAIATMTGVTWVE
                     HLFRRVRAPVWLRPAIGGTVVGLIAEIFPQVLGSGHGAIQLSLDQAFPGGVLLGLVAA
                     KGLASAVSVGSGFRGGLFSSSLLLGSVFGGTAAILIQAASSALGSAAALDQVAYMLVG
                     MASMAAAIIGAPLTMIFLVLEQTGSFVAAMGVMVGVIVASVVVRLTFGYSFATWRFHQ
                     RGVPIRGGYDIGWIQDLTAEKLMRRDFQTVPRGMTVAAFRAQFPLTGAKRVFLVDEQN
                     HYRGMVVTGDAHDPDIDGARGIDELRLAEDNFLLPGQNVRLALNRFVSAEVETLAVVS
                     DAAERHVLGFLTEGYALRRYNEEFERVRADELGERTLFGPA"
     gene            complement(15359..17167)
                     /locus_tag="VGP50_03710"
     CDS             complement(15359..17167)
                     /locus_tag="VGP50_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEV8026509.1"
                     /translation="MSRGRRKSYRAAFIGAGLGILMAASAALPGTGALAATVATTTGT
                     FTGVPSPTLSDVSVFFGIRYAAPPEGNLRWTPPQSPTPPSGTVLASTPGPACPQNAST
                     APLPQSEDCLFLNVYVPPGATPSSHLPVFIWIHGGALVTGTGAQYDPSVMVAENNIIV
                     VTINYRLGALGWLSEPGLHAITPSFFQNPGDAGNYGLMDQQFTMQWVQANIAGFGGDP
                     TKVTIGGESAGGLSVSSNLESLQTAGGLFRSAIIESGGYMLHDLPAEEIYEAIFGPGF
                     DAALGCTPPADAACLRSQSVSAILAAQTAAFGANGISPDFATKVLPFSLDVAFEAGLT
                     FHVPVLQGTNANEGRLFEPLIFPFPAAPVAQVFAAGGPATFDLANPNTFCATPQGTGT
                     PVDCTYPQEINLFLALNGFPAAANTSTFDNTLAQEYPLANFPDPFTGNSPNADEALSQ
                     IFTDLVFACNGSDSNTDLSTFTPVFAYEFNDPQAPPTVGFGTVIIPPNDVFGFPSASE
                     HASELQFLFNFGTTLNAGEQQLAEEMKTYWGNFVNTGNPNTPRLTSTWLPFSAVNMAV
                     QDLVPGPTDPSPFFTFRTEHFCDTTWEPILEVEEEQ"
     gene            complement(17433..18326)
                     /locus_tag="VGP50_03715"
     CDS             complement(17433..18326)
                     /locus_tag="VGP50_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015073385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV8026510.1"
                     /translation="MDPGWELYRSFLAVVQEGSLSAAARRLALTQPTIGRHVAALEAL
                     LAVPLFTRSPSGLAPTAAARDLLPHAEAMAAAAEALRRAASGEAAEERGTVRVTASEI
                     IGAEVLPAMLERFGAAHPAIVVELVLTNRSEDLLRHEADVAVRMARPRQAALVARRIG
                     AMGIGLYAHRRYVEAHGLPNSVEDLRRHRLIGFDRDDSAWRSLRAEELAAARQRFVFR
                     SDSDLAQLAALRAGIGIGGVQHGIARRDGDLVPVLPRAFAFELPIWLVVHEDLRASRR
                     VGLLLDHLAHALTAYTRPAPP"
     gene            18415..19443
                     /locus_tag="VGP50_03720"
     CDS             18415..19443
                     /locus_tag="VGP50_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV8026511.1"
                     /translation="MTQVEDRTALVIGATGGIGGETATALVRDGWRVRAATRDPERAR
                     RDFAALGPVAWIAGDAMRDVDVVAAAKGVRLIVHAANPPGYRNWRGLALPMLKNAIAA
                     AKASGARLVAIGTVYNFGPDAGPVVDERAPQHPLTRKGKIRVEMEEMLQAAATEGVRS
                     LVVRAGDFFGPEAPSAWFNTAMVQKGKPVRFVIYPGRRDVGHAFAYLPDLARTIVRLA
                     AIEARLPAFEIVHFAGHWLARGADIADAIRCVAGRPGAPILPFPSLLLYLAAPFSTTF
                     REMIEMRYLWREPLRLDNRKLEALIGPEPHTPLDDAVRASLEGLGCLAGAAKDAEEAT
                     PREGLRLS"
     gene            19846..20337
                     /locus_tag="VGP50_03725"
     CDS             19846..20337
                     /locus_tag="VGP50_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026512.1"
                     /translation="MSCIELDHPRVLLPPPAQQAWRLAPAAPVRRSLPRLWILMWVLG
                     LLGTAIPAGNQWASSLQSPGPTIRHELARAAAEMRAPAPAARAIDESALLRHFPQHHA
                     TLDTRFWPVAVITLHGLDRAACIDATDVAGRIDGLVVVQLQGYAAPTDCAAVNDMSWR
                     IMP"
BASE COUNT         3314 a         6733 c         7281 g         3033 t
ORIGIN      
        1 agcgccttga aggtggccga gaccttccgc gtgctcgaga ccctctatcc gggccgcatc
       61 gatctcggca tcggccgcgc gcccggcggc gatgcgctga cggcgcgcgc gctgcagcac
      121 gggcccggca cgttcggcgc cgattatttt ccgagccaga tcgccgatct catcggcttc
      181 ctgcgcgaca cgatgccggc cgaccatccc ttcgcgacgg tgcgcgcgca gccccagggt
      241 gcgacggcac ccgagctgtg gctgctcggc tcgagcgacc agagcgcggc ggtcgcagcg
      301 catttcggct gtgccttctc tttcgcgcat ttcatcaccg accagggcgg gcccgaggtg
      361 atggcggcct atcgcaagta tttccagccc tcgccctggc tcgcagcacc ccaaggcagc
      421 atcggcatct tcgtgctctg cgccgacagc gaagccgaag cccgccgcct ggcactgtcg
      481 cgcgagctgt ggcgcctcag gctcggccgg ggcgagctcg ggcccttccc gtcggtcgcg
      541 gaggccgagg cttatgccta tagccgcgag gaccgattgc gcatcgccgt gatgcggcgg
      601 cgcgcggtgg tgggtgcgcc cgagcaggtg cgggaccagc tcctggcgtt gggcgcggat
      661 tatggcgtga gcgaattcgt cgtcgtcacc atctgccacg acaccgccgc gcggcggcgg
      721 tcttacgaat tgctggccga ggctttcggg ctggtgcctc gtgcgccgcc ttgagctccc
      781 tcaaaggccg ggatggtcgg ctgcctcgat ctcctcgtgc ggcaccccgg agcgcagcgc
      841 ctcggcggcg cggcgcagcc ggcgatagga gcggatgctg agcgggatcg aggcgatgta
      901 gagcgcctcg accgcgagca gtgtcgccca gggctcggtg gtcatgaagg cgacgaggcc
      961 gacggtcagc agcagcaccg gcggccacca ctcgatcggc agatggacgc gcttgaagga
     1021 gaaggtcggc acgcggctca ccatgagcgc ggcgacgccg gccacgacga tgccgctgag
     1081 atagggcgag cggaagaggc cgtcgccgaa ctcgaaggtg aggaacatcg ggatcatcac
     1141 cagcatcgcc gccgccggcg ccggcacgcc ggtaaagaaa ttataggcgt aaggcggcag
     1201 ctcggcgccg atctgggcgt tgaagcgcgc gagccgcatg gccgagcaca ccgcgaagag
     1261 cagcaccacg ggccagccga agctcttcag atcctgcatg gtccagagat agagcatcac
     1321 cgccggcgcc gcgccgaagc tgacgaaatc cgacaacgaa tcgagctgcg cgccgaaagg
     1381 cgatgtgccc ttgagcaatc gcgcgatgcg cccgtcgacg ccgtcgagga cggcggcgag
     1441 catgatggcg accgtggccg attcgtactt gttcgagagc gcgaagcgca tcgccgtcat
     1501 gccggcgcag agcgcgagca gggtcagaat gttcgggatc atgcggttga gcgacaggct
     1561 cccgatcggc ttgacgcgga cgccgggccg ccgcatcact gcgcccgccc cgtgcgcggc
     1621 ggctcttgcg agcggcaatc ggcgaccacc gtctcgccgc cgaggacgcg ctggccgacg
     1681 atggcgaaag gcgtccaggc tggcggcagg tagacgtcgg tgcggctgcc gaagcggatg
     1741 aggccgaagc gctggccggc aatgagggcg tcaccctcgc tcaccgtgca gacgatgcgt
     1801 cgcgcgacga ggccggcgac ctggacgacg gcgatgtccg ggccttcgct cgtggcgatg
     1861 cggatcgcca tgcgctcgtt ctcctcgctc gccttgtcga ggcttgcgtt gaagaacttg
     1921 ccggggtggt agacgcgctt ggcgacgcgt ccggtcatcg gcacgcggtt cacatggaca
     1981 tcgaacacgt tgaggaagat gccgatgcgc gtcatcggcg ccgtgcccat ctccagctcc
     2041 gggggcggca ccgccggccc gaccgaaacg acgagcccat ccgccggcgc gatcaccagc
     2101 ccctcgcgct ctggcgtgac gcgccaggga tcgcgaaaga aatagacgca ccaggcggtg
     2161 ccgacgacgc cgatccaacc caaggggtcg tagatccaga agagaaccgc cgtcgccacc
     2221 agcgcgatag cgatgaagcg ccacccgtcc ggatgaatgg gagccagcac ctgtctcagc
     2281 acgcgaactc tctaatatgc agcgagaaga gggacgacgg agaaagaagg gctctacttt
     2341 gtctctttgc tccggtcaaa tttctcttac cgcccctcgc gccgccaggc gaagaggaag
     2401 gcggcgagcg ccaggaggag tgccaggaaa cccggcagaa gcgacacttc cttgacgccc
     2461 gtcacgacat agtcgccatt ggcgcggaaa cccatccagc tgtgtccggc gctcgcgcgg
     2521 tcgggcgcga cgcgccgcag gtcgggcagc gcgccgggac cgacccagaa ggtcccgccg
     2581 ccggtcgcct cgaccagggg atgcagcttc tcgtcggtgg tgcggacatc ggcgagctcg
     2641 atcggattca aagcgcccgc ggccgcgagc gccgtccgtg tgccgtcctc gacgcgatag
     2701 agaccgctct cggcgaccgg catgctcccg gtgctgcgcc cgccgctgtc gggcgtgagc
     2761 tggagcgtct cgagcttgcc gtcgggtccc gtgacctgga cggggcggtc gtccttctcg
     2821 agcgactggc gcgtcacgat gaggcggttg ccgtcgacca cggcgcgcag gtcgttctcc
     2881 tcgagatcgg gctccttcat gagccagtag acgacgcggc gcagcagctc cgattgcggg
     2941 ccgccgcctt cgaagccgcg cgtccagagc cacagctcgt cggacaggag ctgcgcgacg
     3001 cggccatcgc cggcgcggtc gagcacgagg agtgggcggt cgttgaggcc ggtcatgatg
     3061 gtgacgccgt gatgcggctc ggcgtccaca tgacggaacc agcgccccca tttgggtggg
     3121 gtcgtgccgt cgggcgaatc gccgggcagg ccggcggtga cgggatgacg ccggccgatc
     3181 tcgctcacgc gcggcaggaa gccttcctcg aagacgtttc ccgtgggctc cgccggcagc
     3241 accgcgccca gcgggctgcg atagaggctc aagggcgtgc cgaaggtcgg gcccgcggct
     3301 tcgagcaagg cgccgccttt gcgcacatag cgcgcgatgt tctcgagata ggcctgcggc
     3361 aggatgctgc gccggcggta acggtcgaag atgatgagat cgaattcctc gatcttcacg
     3421 tcgaagagct cgcggatcgg gaatgcgatc agcgacagct cgcggatggg cgtgccgtcc
     3481 tgcttctccg gcgggcgcag gatggtgaaa tggatgagat cgaccgaggg atcggatttg
     3541 aggatgttgc gccaggtgcg ctcgccctga tgcggctcgc cgctcaccag cagcaccttg
     3601 agccggtcgc gcaccccgtt gacgatgaga gcggcgcggt tgttgtcgag ggtgagctca
     3661 tgcggacccg gctcgacctc gagctcgatg acgttgggtc cgccatgatc gacggtgatg
     3721 gtcaccggca cgtcgcgccc gacgggcaca aggcgcgagc tcggcgcgcc gccatccttg
     3781 cgcagggtga gccgggcctg gccgttctct gctccctcgg gcaggtcctc gacgcgcagc
     3841 gtgagctgca gctccttgcc gacgagaccg aagctcggcg catccttgac cacgaggcgg
     3901 cggtccgctt cgcccgggcg gcccgtcagc aggacatgga gtggcgcatc gaaggcgcgg
     3961 cgctcaggcg gcggcacgtc atgcacctcg ccgtcggtga tcatgacggc gccggcgatg
     4021 cgctggcgcg gcacgtcggc gagcgcccgc gtcagcgcgg tataaagctg agtcccggag
     4081 tcgataagcc cggccttcgg atccggcgcg ccggcatgaa cgacgcgcag atcgatgtcg
     4141 ggaaactgct gcaggcgctg cgtgacatgc tgcaacgcct cgtcggcgaa acgacggcgc
     4201 tcgccgatgc gcatgctcgg cgaatcgtcg acgacgacca cggcgacgtc atgctgcggc
     4261 tggcgctcct cctcgaccag cgaggggttg gcgaggatca ccaggagcac gacgaacgcg
     4321 gcgaggcgcc aggcgaggcc gcgcgcgcgg cgccacaggc cgaaggcgag aaagagaatc
     4381 gccagccccg caaaggcgat gatcgccacc cagggcagga gcggcgcggt ggcgatgctg
     4441 tagccggcga tcgtcattgg cccagcctct cgaggattgc cggcacatgc acctggtcgg
     4501 tcttgtagtt gcccgtgagt gcgtacatca ccaggttgac gccgaagcga taggccatct
     4561 cgcgttgcgg ctcgccgccc ggcacggccg ggtagagcgg ctgcccgcgc tggtcggtcg
     4621 cccaggcgcc ggcccagtca ttgccgccga cgacgatgct ggagacgcca tcattgacct
     4681 ggtcttccac cgtctcgacc cagaccgagc cgccggccca acggcctggg aagtcctgca
     4741 tcagatagaa ggatttcgtc agcacatggt cgggcggcac gggcaccagc ggaggaatgt
     4801 tgagcccggc ggcgagcttc ctgagtcgcg cctgggtgcc tgccgcggag aagggcgtgc
     4861 tctcgccctg gtcgcgcgta tcgaagaaga tggtcccgcc cgtcgcgaga taacggttga
     4921 ggcgctcgac cgccttgccc gaagggagct gctgtccctc caccaccggc caatagagca
     4981 gcgggaagaa gatcagatca tcggtctcgg gatcgacttc catgggggcg gccgcctcga
     5041 cggcggtgcg atggctcaac acgtcggtaa gcccggcgag gcccgcgcgc gattcctcgt
     5101 cgacctcggc gatgccggtg cgcacatagg cgagatgcag ctcgctcgtc gcctccacgg
     5161 tgaaggcgtc atcggcgaaa gcgggcgtcg cgccgagcgc cagcgcgaga agaaggagcc
     5221 cgaccgtcgc accgcccgcc cgccgcggca ggcgcggcag caggccgcgg agcgcatagg
     5281 cgatcagcag gtcgatgagc gccagcgcga gcgcggcgcc gagcaggccc ggacggaagt
     5341 cgatctcgtt gctgcggccg tagccttcgc gcgcgacgcc gggcggcaag gcgccgatcg
     5401 cggcgaagcg cttgaccgcg ccaaagaggt tgagggcccg acgcgcgtcg ggtgttccat
     5461 agaagccggg aggatggctc ggcgacgcaa tcgtgttgcc aaaggcaccg gcggcaatgg
     5521 tctgggcgct ggacggcgcg cgctcgagcc gcccgaagcc gtccagagtc tcgaccggcg
     5581 gcagcgcggt atcgctgctc cccgaaacgc cctgactgag cgccgcgatg cggcgcagca
     5641 tgctcacgaa gagaccggag atcgcgagat tcgaccattc gggatcggcc gtggtgtgca
     5701 ccaggacgag ccagcctttg ccgtgcttgt cggcggtaac gagcggtgtg ccgtccgtca
     5761 gccgcgccca ggtcttggcg gcgagatcga cagtcggctc ggccagcacc tggcgcgata
     5821 cggtcacgtc atcgggaatc tcgagaccgg cgaacggact gtcggcgcca aagggcgcga
     5881 gcttcgccgg cttctcccag gatagcgcgc cgcccagcgt ccgtccgccg cgccgcaagg
     5941 ccacgggcag caggtcatcg tccacctgtt ccgcgagatg cggcccggca aagcgcagca
     6001 cgacgccgcc attctccatc cacttgccga gcgcggcctt ctccgccacg ctcgccggag
     6061 cgacatcggg gagggccagc acggcaatct cgcctttgag cagggtgtcg gcgtcgccgc
     6121 ggcgcacctc gctgaagggg ctcagtgcgc gctcgagata ataggcgccg ctcaacaacg
     6181 gctgcgccgc tgttccctgc tgccgggcga cgacgccgac gggacggcgg cgccagcgct
     6241 cgtccagcag caggacggcg cccgccgagg tctcgccttc gatctggatc gccgcggcgc
     6301 ggtttcgcag ctcgctcggc attgccagat gcgccaccac atggtcgatg gccggatcga
     6361 gcgtcacctc ctggcgggcg agaagccgcg aatcatcgcc gaccgcgcgc acgaagaggc
     6421 gatcggcgcc ggcagcatcg gcgcgccgca cggtgacggt gagatcctta tcgtccatgt
     6481 cagcgggcgc gaggaggcgc ggcaagttgc ccggctcgtc ggcaaccacg cgcaggctgc
     6541 cgaagcgctg cagcccgcgc gcgaaatcct gcgcctcgcc gccatccacg ccatcgctca
     6601 gccagacgat ctcggcgtca ttgccgaccg cgagctgcgc caggcgatca agcgaggcgg
     6661 ggcgattcgg tggccagggt tgcggcctca ggtgatcgag ggccgcccgc gcatcggcgg
     6721 ggcgcagcac ggagagcggc ggcggcgcga tgtccccggg cgccgtcgtg gtgcccagca
     6781 tcacgatccg ccgatgctcg cgttcggcct ggtcgatcag tcggtcgagc atggcgcggt
     6841 gctcgtccca gtgcggagcg gcgctccagc catcatcgac gacgagaacg agcggtccat
     6901 cgccgccgag ctgcgcgctc ggattgagca agggatgggc gagcgcgaga atgacgaggg
     6961 cgatcagcgc catgcgcatg agaatgagcc acagcggcgt cttcgccggc gtctcctcgc
     7021 ggggcgtgag cccgagcagt aggcggagcg ccgggaagcg gacgcggcgc ggcgccggcg
     7081 gcgtgacccg gagcagccac cacagcgccg gaagaaccag aaggccccag aggagccacg
     7141 gtgctgcgaa ggcgagggga ccgagagcga gcatggtcag cgtccgtcgc tcgcaccccc
     7201 caccctaacc cttcccctca aggggggccg ggacaagtgg gagattgcaa ggagggaggt
     7261 gaaccgccgc gctgacatgg ccatcatcga cgacccgggc gccgccgcgg ctcggcaagc
     7321 gctccgtaga gagcgagcag cgccgtatgg ggcggccggt ccgtgcggtg ggtaccgaag
     7381 ctccaaccgg tggcgcgcgc gatggcggcg agaccttcgc gatgcttggc aagccgcgcc
     7441 tgatagtcgt cgcgcaccgt ctcgacgcgg ctgatgagga gcggatcctc cgcctcgaag
     7501 ccctcgaagc gcacgcgacc catgaaaggc agggtctcct ccgccggatc gagcacctgg
     7561 agtaggtgac cgcgtaatcc gccggaggcg aaacggccga ccagcgcgtg gatggtctcg
     7621 agcggcgcca ggagatcgcc gatccagacg agctgaccat gacgcggcag aggctgaaac
     7681 tgcggcagct ccggacccga tccttgccgc tcgagggtga ggcccagtcg gttgagcacg
     7741 gcgcgaccgc tggccggcgg cacgcccgag cccaaaagcg tgacgcgctc gccgccgcgc
     7801 acgaggagcg ccgccagcgc caggagcaga atgtccgtac gctggcgttt gctcggcagc
     7861 gcggcggtcg aggcgtaatc catcgacggc gagctgtcgc gccacagcca gacgctctgc
     7921 gcggcctccc actccgtctc gcggacataa aggtgctgtg attttgcact ctcccgccag
     7981 tcgatgcgcg gcaccgggtc gcctggcgaa tactggcgga attgccagaa agtctcgccc
     8041 tgcccgacac gccgccggcc gtgcacgccc tgtgccaccg tcgccgcgac gcgctcggcc
     8101 gcgacaagga gcggcggcag actcgccgcg agctgctccg cgcgatgctg aagcgcggcg
     8161 ggacccggca tcaggtgaac ggtcccacga gccgatcgat gatatgcgac atcgtgatgc
     8221 cgtcggcccg cgccgcgaag ttgagcgcca tgcgatggcg cagcaccggc ggtgccagcg
     8281 ccaccacgtc gtcgacggat ggcgcgagcc gcccctcgag cagcgcccgg gcacggcagg
     8341 cgagcatcag agcctggctc gcgcgggggc ccgggcccca ggcgacgtgc tgcttcgctt
     8401 cggcgaggct ggtgtcttcg gggcggccca tgcgcaccag cttcagaatt gcctcgacga
     8461 cgctctcgcc cacggggatg cgccgcacca ggcgctgggc agcaagcagt tcgcccgccg
     8521 tcagcaccgg cgtcgccttc tcctcggtga tgccggtcgt cgcgatcagc atctgccgct
     8581 cggcgtcgag gtcggggtag ccgacatcga tctgcagcag aaagcggtcg agctgcgcct
     8641 cgggaagagg atatgtcccc tcctgctcga gcgggttctg cgtcgccagc acatggaacg
     8701 ggcgcggcaa cggatgatac tggccggcaa ccgagacgcg cccttcctgc atggcctgca
     8761 gcagcgccga ctgtgtgcgc gggctcgcgc ggttgatctc gtcggccatc agcaactggc
     8821 agaacaccgg gccagggatg aagcggaacg agcgccggcc gctctcgctt tcctcgagca
     8881 cctcggagcc caggatgtcg gccggcatca ggtcgggcgt gcattggatg cgtttgtcct
     8941 cgagcccgag caccgtgccc agcgtctcga cgagccgcgt cttggcaagg ccgggcaccc
     9001 cgatcagcag cgcgtgtccg cccgcgagga gcgtgatcag cgcttgatcg atcacctcgc
     9061 gctgcccgaa gatgacgcgg ccgatgcgct cgcgcacgag cgccaagcgt ttgcccaagg
     9121 cgtcgatctc ggccacgagg tcggtgctgc cggcgccggt cgataggtta tccgtgatgc
     9181 tcacgacgac tgttccttgt tgcacacgtt ccctcccgcc ttgtgggaga gggggcccgg
     9241 cgcaaaacgc cgggaggata gggggtcagt gctccggcat gcggtcgcca acgtcccaag
     9301 atcatagatc atgacggata agccggcgcc atcaaagcgt ctcgatcggg gggcggcaat
     9361 gccgaacgca gaacgcgtcg aacatggtaa acatccaaga cagcccatcg attgcggcca
     9421 ttttgacata cgcattgctc gtgacggcac ctggtattat cgcgattcgc cgattgcccg
     9481 catgccgctc gtccgtctct tcagcacggt gctgcggcgc gaccccgatg gcaccttctg
     9541 gctggtaacg cccgtcgagc gcggtcggat cacggtcgaa gacgcgcctt tcgttgcgct
     9601 ggggctgacg gtggaaggcg aggggcggtc gcagagtctg atcttccgca ccaatattga
     9661 cgacactgtg gcagccgacg gggcgcatcc gctccgggtc gtgaaccatc ccgagaccgg
     9721 cgagcccaac ccctatattc tggtgcgcga cgggctggaa gcgcgcgtga gccgctcggt
     9781 attctacgaa ctggtggcgc tcgggcgcga agagcgggtg ggcgaggaga ccctttacgg
     9841 cgtatggagc aagggcgagt tcttcccgct gggccgtctg gatggcgagg cctgacgccc
     9901 gatgacgtgc ggcggcgctt cgcccgtgcc gttgcgggca cgcgcgccag cgaccggcag
     9961 acgatcgctc tcatgcgcgg cgatcactcg ctcgtgcctg gcggagcacc gccggcgacg
    10021 gtactgacgc cggcggcagt gctcgtgccg ctggtgatgc ggccggagga gctcacggtt
    10081 ctgttgacgc agcgcacgcc gcatcttgcc gcccatgccg ggcagatctc cttccctggc
    10141 ggcagcgtcg agccggttga tgtcgattcc atcgacacgg cgctgcgcga gacgacggaa
    10201 gaggtgggcc tgccccgcga tcacgtcgag atcgtcggcc gtctcgacac ctatgtgacc
    10261 agcaccggct tcgaggtcac accggtggtc gggttggtgc gggcgcctta tccgatgaag
    10321 ctcgaccctt tcgaggtcgc cgaggtgttc gaggtgccgc tcgccttcat catcgatccc
    10381 gcgaatcatc agcgcgagag tcgcgagttc aagggcagaa tgcggacttt cttcgtgctg
    10441 ccctaccaga accgctacat ctggggcgcg accgccggca tgctggtgaa tttgtcggag
    10501 gtgctgggcg ggctcgcatg agtgaggtcg acgctcccgt cgatcgcctt gcgccgcagc
    10561 cctggatgac ggagccggcg acgcacgccg tgcttgcggc gctctccgat gccggcggcg
    10621 ccgcgcgctt cgtcggcggc gccgtgcgcg acgcgctgct gggccgcccg gtcggcgata
    10681 tcgatatcgc gacgccgctc tcgcccgagg aggtgacgct gcgcctcgag cgcgcgggtg
    10741 tcaccgtcgt gccgacggga attgcgcatg gcacggtgac ggccgtcgtg ccgccgcggc
    10801 atttcgagat cacgactctg cgccaggatg tcgagacctt cggccggcag gcgcgcgtcg
    10861 ccttcattgc cgactggacg ggcgatgcag ggcgacgcga cttcaccatg aacgcgctct
    10921 ttctcgacat cgacggccgc gtgttcgatc cggtcggcgg tctcgaagac ttgcatgcgg
    10981 gccgtgtgcg cttcgtcggc gatccggcga cgcgcattcg cgaggacgtg ctgcgcctct
    11041 tacgcttcta ccgcttctat gcgcattacg ggcggggcga ggccgatggc gcggcgcgcg
    11101 ccgcctgtac ggcgctcgcg ccgctgctgc cgggcctttc gggcgagcgc gtcgcggccg
    11161 agacgttgaa gctgctcgct gctcccgatc cgctgccgac actcgcgatg atggccgagg
    11221 atggcgtgct cgccgtcctg cttcccgaag cgcagcggcg cgaccgactc gcgacgctcg
    11281 tgccgctcga gcccacgccc gatccgctgc gccgcttggg cgcgctcgtc gcgggcgatg
    11341 tcgcccagct tgccgagcgg ctgcggctct cgaatgagca gcgcgaccgg ctgacggcac
    11401 tggcgcagcc gccctggccg atcgacctag cgggcgacga tgccgttcag cggcgcgcgg
    11461 tctatcatct cggcgtcgag cgctatcgcg atctcgtgct gctgcgcgcc gccgagaccg
    11521 gcgagatcga gcgcgttgct gcgctgctgg ctctcgcgga atccgcatgc ccgccgccgt
    11581 ttccgctcaa gggccgcgac gtgacggcgc tgggcgtgcc gccgggtccg gaggtcgggc
    11641 gccttctcgc cgcggtcgag gcatggtggg aagagggcga cttccgcgcc gaccgcgccg
    11701 cgtgcctcgc ggaggtcgcg cggcgggtga acgagcgctg agacgagaaa gggcgcgacg
    11761 gctaggcttc ttcgtccggg tcctcgtcct cgtcttcctc cagcgagcgt cgccagccat
    11821 ccgccgtcag ctgctcgagc gcggcataat atgcttcgtg cgaaactgat tccgccgtcc
    11881 ccgctcctgc tcccgctccc gctccgacat cgtgcatcag ctgggtcaga tgctcgaaca
    11941 gcaccgttcc agccgcgtga agggcttcgt gtcgatcgag tccgtcggcc atcaagcggt
    12001 cgatagcgcg gcgcaccggc acctcgtcgc ccagcgcaat ctgattctcg acggcgacat
    12061 gcattatggc gtgcaacatc tgattgggaa cgcggatgcc ggcgccgcgg tgatacgcct
    12121 cgatgaccaa acggcgttcg tcttcgcttc gcgccagcca ttccttagga ttcggcgcga
    12181 cctcaggatc atagggcgcg gcgccttccg ggagctcgag ctcgtcgtcg agtatgtcgt
    12241 cgtcatccgc gtcgtcctcg tcgtcgtcat cgagggcgac gtcgaagccg tcgtctccga
    12301 gtcgttcttg cagatgcctg aggcgtcgat cgatctgctt cgccgactca tgcggccccg
    12361 ggatatagaa gggcttgccg tcgagcccga attcgaagct cacgtcgcag gcctcggcgg
    12421 agatctcgcc gaacaccggc tcgacgccgg cgaagtcggg gtgcggctcg aggccgagcg
    12481 atcgcgcata ggcgacggtg tcgcgcagca gctttcgcgc ataagcggga tcgacgtcgg
    12541 agagcggtac gtcttgtccc atgatgtcca tgtattcgtc gagttccgat ggctcgaacg
    12601 ggcgaaagaa gacgtccttg acgccgagac agaagacgtc gaggaggaaa ccggcgaccg
    12661 ccaattgacc gtcgctctgc tggcgggcca ggatcatcat tccgttgccg cgctcgaaca
    12721 agccgtcttg caccatgcat gagcggagcg gcatggccgc gagccggcgc acccgtgtgg
    12781 ccaatggcag gttgctcgcg cccccgagct tgcgtcgctc cgccaggact tgcttgcggc
    12841 gtttggccct ggcttccttc cgccgaagaa ttttcgatga catccctcac ccccgtgcag
    12901 gaactgggat aacgctagca tatctcccga acatggtcgc gggctcgctc atgccggaag
    12961 ctgctgctca atgccgcgac ccgagcagcg agcgttcagg ccgctgcgag agataggccg
    13021 agagtgcggt cagcgccgcg gcataagcga atgcgatgcc gaaggccgca gagaagggca
    13081 gtggcggatt ccggccgagc aacgcgctga gatagaggaa aaaggtcggg agcggaatgg
    13141 cggcgcgcgc caggacggcg gacgcgacgg gcagcgaggc cagcgcgaag acggcgccga
    13201 ggagcaccgc ttcgagccat ggcggcaaca ggtcgttgct gtcgtcgacg cgctgggaaa
    13261 gcgcgccgtc agtgtaggcg gcgacgacga gtccgagcac gagggcgacc cagacgcgtc
    13321 gcaggcggcg ccggcgcgcc tgcgccagga cgtcgctcga atagacggga gccggcatct
    13381 cgcctcctta cgccgggccg aagagcgtgc gctcgcccaa ctcgtcggcg cgcacgcgct
    13441 cgaactcctc gttgtagcgg cgcagcgcat agccttcggt gagaaagccg aggacatgtc
    13501 gctccgccgc gtcggacacc acggccagcg tctcgacctc ggcgctcacg aaacgattga
    13561 gagcgaggcg gacattctgc cccggcagca gaaaattgtc ctccgccagg cgaagctcgt
    13621 cgatcccgcg cgcgccgtcg atgtcgggat catgcgcatc gcccgtgacg accatgccgc
    13681 ggtagtgatt ctgctcgtca accaggaaca cgcgcttggc gccggtgagc ggaaactgcg
    13741 cgcggaaagc ggcgacggtc atgccgcgcg gcacggtttg gaaatcgcgg cgcatcagct
    13801 tctcggccgt gaggtcctgg atccagccga tgtcgtagcc gccgcggatc gggacgccgc
    13861 gctgatggaa gcgccaggtg gcgaaggaat agccgaaggt gaggcgcacc acgaccgaag
    13921 cgacgatcac gcccaccatg acgcccatcg ccgcgacaaa gctgccggtc tgctccagca
    13981 cgagaaagat catggtgagc ggcgcgccga tgatagcggc cgccatcgag gccatgccga
    14041 cgagcatgta ggccacctga tcgagcgctg cggcgctgcc gagcgcggac gaagccgctt
    14101 gaatcaggat cgccgcggtg ccgccgaaga cgctgcccag tagaagcgac gagctgaaaa
    14161 gcccgccgcg gaagcccgag ccgaccgaga ccgccgatgc cagtcctttc gcggcgacga
    14221 ggccgaggag cacgcccccg gggaaggcct ggtcgagact gagctggatg gcgccatggc
    14281 cgctgcccag cacttgcgga aagatctcgg cgatgaggcc gacgaccgtg ccgccgatcg
    14341 cggggcgaag ccacacgggt gccctgacgc ggcggaagag gtgctcgacc caggtcacgc
    14401 cggtcatggt ggcgatggcg atcgccgcgg cgacgacgcc gaggccggcg aagagcgcgt
    14461 aatcgagcgt ggagacgtgc accggcgtat cgagcgcgaa gatggaatcg gcgccgaaga
    14521 tgccgcgcac taccagcgtc gccgagagcg cggcgacggc gatcggcgcc agcaccggcg
    14581 ccgagtagcc gccgatcacc agctcgaagg cgtagaaggc gccggcgagg ggcgcgtcga
    14641 aagcggcggc gatggccgcg gcggcgccgc agccgacgag cgtgcgcaga tcgccgcggc
    14701 gcatgcggag ccgctgaccg acatgcgacg cgaaacccgc gccgagctga gtatagcccg
    14761 cctccattcc gaccgatgcg ccgaagcccg acgagattat ggtgaggccg gtcaggagcg
    14821 cgctgtcggt gagcgacatg cgtccgccga agagcgcgtt ggcctcgatg gcgtcgacgg
    14881 tctcgcgcgg ccgcctgcgg cgaataagca tggcagcgct gccggacaag aggccgccga
    14941 ccaccggcac gatgagcaag cgccaccacg cgatggcgcc gccggcgctc acatgggcct
    15001 ggaagggaat ggcgaaattg atcgcgtgga agagctggac gaattgctcg atggcgccga
    15061 cggcgatgcc ggcgacggcg cccaggagcg cgctgatcgc gaggagcgcg atctcgttgt
    15121 cgcggaaatt gcgctgggcg acgcggccga gccagaggcg cgcgcgcagg cgcacgctat
    15181 gccaggggcg cgtcggcgtc gcggaactca tgcggggaat aatgacgttg cgccgcgttc
    15241 tttgccacaa gccgcggcgg atttcagcgc gaatccgggc cgtttccgcg cgttttcgcc
    15301 caaaaaaaag acgccctgcg cgatcggcag ggcgtcttcg gacgaaatcg accggttttt
    15361 actgctcctc ctcgacctcc agaatcggct cccaagtcgt gtcgcagaaa tgctcggtgc
    15421 ggaaggtgaa gaacggagac gggtcggtcg gaccgggcac cagatcctgc accgccatgt
    15481 tgacggcgct gaagggcagc cacgtcgacg tcagccgcgg cgtgttggga ttcccggtgt
    15541 tcacgaagtt gccccagtaa gtcttcattt cctcggcgag ctgctgctcg ccggcattga
    15601 gcgtcgtgcc gaagttgaag aggaactgga gctcggaggc gtgctcggac gcgctcggga
    15661 agccgaacac gtcgttgggc gggatgatga cggtgccgaa gccgaccgtc gggggcgcct
    15721 gcgggtcgtt gaactcatag gcgaagaccg gcgtgaaggt ggagaggtcg gtgttcgagt
    15781 cggagccgtt gcaggcgaag accaggtccg tgaagatctg cgacaacgcc tcgtcggcat
    15841 tgggcgaatt cccggtgaag gggtcgggaa agttcgccag cggatattcc tgcgccagcg
    15901 tattgtcgaa ggtgctggtg ttcgccgccg ccgggaaacc gttcaaggcg aggaagaggt
    15961 tgatctcctg cggataggtg cagtcgaccg gggtgcccgt gccctggggc gtcgcgcaga
    16021 aagtgttggg attggccaag tcgaaggttg cggggccgcc cgccgcgaag acctgggcga
    16081 cgggtgccgc cgggaagggg aagatgagcg gctcgaagag ccggccctca ttggcattcg
    16141 tcccctggag caccgggaca tggaaggtga ggcccgcctc gaaagccaca tccaggctga
    16201 agggcaggac cttggtggcg aagtcgggcg agatgccgtt cgcaccgaag gccgcggttt
    16261 gagcggcgag gatcgccgag accgactggc tgcggaggca agccgcgtcc gccggaggcg
    16321 tgcagccgag cgcggcgtcg aagccgggcc cgaagatcgc ctcatagatc tcctcggcag
    16381 ggaggtcatg aagcatgtag ccgccgctct cgatgatcgc gctgcggaag aggccgcccg
    16441 cggtctggag cgattcgaga ttggacgaga cgctgagccc gcccgcggat tcgccgccga
    16501 tcgtcacctt ggtcgggtcg ccgccgaagc cggcgatgtt cgcctgcacc cattgcatgg
    16561 tgaattgctg gtccatcaag ccgtaattgc cggcatcgcc cgggttctgg aagaagctcg
    16621 gcgtgatggc gtggagaccg ggctcggaca gccagccgag cgcgcccagt cggtagttga
    16681 tggtgacgac gataatgttg ttctcggcca ccatcaccga ggggtcatac tgcgcgcctg
    16741 tgccggtgac gagggcgccg ccatggatcc agatgaatac cggcagatgc gaggacggcg
    16801 tcgcgccggg cgggacataa acattgagga agaggcagtc ctcggattgc ggcagcggcg
    16861 ccgtgctggc gttctgcggg caggccgggc ccggggtcga ggcgaggaca gtgcccgagg
    16921 gcggcgtcgg cgattgcggc ggcgtccagc gcagattgcc ttcgggcggc gcggcatagc
    16981 ggatgccgaa aaagacgctg acgtcgctca gtgtcggcga cggcacgccg gtgaaggtgc
    17041 ccgtggtcgt ggcgaccgtg gccgcgagcg cgcccgtccc cggcagcgcc gccgaagcgg
    17101 ccatgagaat acccaagccc gcgccgataa aggcggcgcg ataagatttc ctccgtcctc
    17161 tgctcatcct ctgctcctcc cggttaagct ttcccagacc tgtgccccga gcacgctgcg
    17221 gctggtacgc ggcgttattg gcacgacgga cctggctccg tcgcgtcacg atttcgccgt
    17281 cgatggtgtt cttgacgccc gagtcgagcc cgggaactta ttgaccgaaa atcacaatgc
    17341 gcttttgccg cgatgatgcc ggccgcccaa ttttggaaca actagaatct gtctaatgga
    17401 cgtgccgggc gcggtaatat gaaagtgatc attcagggtg gggcggggcg ggtgtaggcg
    17461 gtcagggcgt gggcgaggtg gtcgaggagc agcccgacgc ggcggctggc gcggagatcc
    17521 tcgtggacca ccagccagat cgggagctcg aaggcgaagg cgcggggcag caccggcacg
    17581 agatcgccat cgcgccgggc gatgccgtgc tggacgccgc cgatgccgat gccggcgcgc
    17641 aaggccgcga gttgcgccag atcgctgtcg ctgcggaaga cgaagcgctg gcgcgccgcc
    17701 gcgagctcct cggcgcgcag gctgcgccag gcggaatcgt cgcggtcgaa accgatgagg
    17761 cgatggcggc ggaggtcctc gacgctgttc ggcaggccgt gcgcctcgac atagcggcga
    17821 tgggcgtaga ggccgatgcc catggcgccg atgcgccgcg cgaccagcgc cgcctggcgc
    17881 gggcgcgcca tgcgcacggc gacatcggcc tcgtggcgca gcaggtcctc ggagcggttg
    17941 gtcagcacca gctcgacgac gatcgccgga tgcgccgcgc cgaagcgctc gagcatcgcc
    18001 ggcagcacct cggcgccgat gatctcgctg gcggtgacgc gcaccgtgcc gcgttcttcc
    18061 gcggcctcgc ccgaggcggc gcggcgcagc gcctcggccg ctgccgccat ggcctcggca
    18121 tggggcagga ggtcgcgcgc cgccgcggtg ggcgcaaggc ccgagggcga gcgcgtgaag
    18181 agcggaacgg cgagaagcgc ttcgagggcg gcgacatggc ggccgatggt cggctgggtg
    18241 agcgccaggc gccgcgccgc ggcggagagg ctcccttcct gcacgacggc gaggaaggag
    18301 cggtagagct cccatccggg gtccatacgg aaatgtatag caactagacg aagttcggca
    18361 atttccggtt gaggtggtcg gacgcatctt tggcgggcga aaggagcacc aaccatgacc
    18421 caggtcgaag acaggacggc tctcgtgatc ggcgccaccg gcggcatcgg cggcgagacg
    18481 gcgacggcgc tcgtcaggga cggctggcgg gtgcgcgcgg cgacgcgcga tcccgagcgc
    18541 gcgcggcgcg acttcgcggc gctggggccg gtcgcgtgga tcgcgggcga tgccatgcgc
    18601 gatgtggatg tggtcgccgc ggccaagggc gtgcggctca tcgttcatgc ggccaatccg
    18661 ccgggctatc gcaactggcg cggcctcgcg ctgccgatgc tgaagaacgc gatcgccgcc
    18721 gccaaggcga gcggcgcgcg gcttgtggcg atcggcaccg tctacaattt cggtcccgac
    18781 gccgggcccg tggtcgacga gcgcgcgccg cagcatccgc tgacgcgcaa aggcaagatc
    18841 cgcgtcgaaa tggaggagat gctgcaagcg gcggcgaccg aaggcgtgcg ctcgctggtg
    18901 gtgcgcgccg gcgatttctt cgggccggag gcgccgagcg cgtggttcaa cacggcgatg
    18961 gtgcagaagg gcaagcccgt gcgcttcgtc atctatcccg gacgccgcga cgtcgggcac
    19021 gccttcgctt acctgccgga tctcgcgcgt acgatcgtgc gtctggcggc gatcgaggcg
    19081 cggcttccgg cgttcgaaat cgtgcatttc gccgggcact ggctcgcccg cggcgccgac
    19141 atcgcggacg caatccgctg cgtcgccggc cggcccggcg cgccgatcct gccgtttccg
    19201 tcgctgctgc tttatctcgc cgcgcccttc agcacgacgt tccgcgagat gatcgagatg
    19261 cgctatctct ggcgcgagcc gctccggctc gacaaccgca agctcgaagc gctgatcggc
    19321 cccgagccgc acacgccgct cgacgacgcg gtgcgcgcga gcctcgaagg gctgggatgc
    19381 cttgccggcg cggccaagga cgccgaagaa gcgacgccgc gcgaaggatt gaggctttca
    19441 tgacggtttg gaaatcggcc gcgggcggcg ccaccgagag gcgccgcgtg tcgcggaagt
    19501 cattaatcga ttcgagtgca tcctgctcgc cggccgcggt tgcagcgacc gggcgcgtcc
    19561 tgcgctcgac gaggcgcacg cccaagcgtt cctccaggga ggcgcaccat ctcacggacc
    19621 cgcggcggcg acaggcccag tcgcttcgtc gccttggcga agctcttctg ctcgagcacc
    19681 gcgaccaacg cagtcgtctc ggcaaacccg ggagcggcta tcgcgctgcc tcattctttc
    19741 gtatttcaac atagcctatt cagattacgc ggtcttctga gcggcccgcc ccgggtccat
    19801 cttctcctcg agggcgcgaa agcagccccg ctccaggaga cggccatgtc atgcatcgag
    19861 ctggaccacc cgcgcgttct tctgccgccg ccggcgcaac aagcctggcg cttggcaccg
    19921 gcggcaccgg tgcgccgctc gctgccgcgg ctgtggatcc tcatgtgggt cctcggcctt
    19981 ctcggcacgg ccattcccgc gggaaaccaa tgggcatcgt cgctgcagtc gccggggccc
    20041 acgatccggc acgagctcgc gcgggccgcc gccgagatgc gcgccccggc gcccgcggcg
    20101 cgcgcgatcg acgagagcgc tctgctccgg catttccccc agcaccacgc gactctcgac
    20161 acgcggttct ggccggtggc cgtgatcacg cttcacgggc tcgaccgcgc ggcctgcatc
    20221 gacgccacgg acgtcgccgg acgcatcgat gggctggtcg tcgtccagct ccagggatac
    20281 gcggcgccga cggactgcgc ggcggtgaac gacatgagct ggcggatcat gccgtgagcg
    20341 ggcggtagaa ggcagaagat t
//
LOCUS       DASYNS010000118         2895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_650920, whole genome shotgun sequence.
ACCESSION   DASYNS010000118 DASYNS010000000
VERSION     DASYNS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650920"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VGP50_03730"
     CDS             <1..1123
                     /locus_tag="VGP50_03730"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV8026513.1"
                     /translation="DVIKVEPVAGGDPWRAIAARPGMPETPHNFAWTLEGRNKRSIAL
                     DLKQPDGQRVIRCLAAAADIFITNLPLRVRSALGIAYEQLAPANERLVYASFTAYGES
                     GMEAGTPGFDTTAWWARSGLMDRVRADEESLPPWSVPGMGDHPSAVAITAAILMALYR
                     RERTGKGGYVGSSLIANGAWANAVYIQAALCGARFPERPERSRAVSALRNYYRCADGR
                     WLMLSLSAAQQATSWPAFARCLDRPDLVSDPRFATPIARREHAMALIALLDAVFRTRE
                     SVDWYCRLADAGLTAALISTMDEVARDEQMRESGAFVPMLAAGGAEMTVSSPFWLAGE
                     PKRAAQPAPALGAHTDEILRATDFSEAEIARLRAQGVIL"
     gene            complement(1149..>2895)
                     /locus_tag="VGP50_03735"
     CDS             complement(1149..>2895)
                     /locus_tag="VGP50_03735"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026514.1"
                     /translation="SRAQAGWVIGVTCAFALALGWMFFWMAAQSRQIVIDDTYQSSSN
                     LALSVEQFVARTMETIELSQRIVLEEIGPEGRRASAEIRRLLAARVRQSPQITMLAVI
                     GRDGRVQASSTTAAQAPLDAAREEYFVAARNNNALHFTVEKDIPAQRKPRVFVSRRFD
                     RPDGSFAGVIVATLSADYMQQFFSTLHVGEYGIIALETTDGTMLLRQPDLDGYVGRNF
                     GTSPLFKEWLPWASSGVFVMPYEVDGRLRVVGYQRVEKQPLVVQVALAKDEALAHWRR
                     TTIVQAAVALAILVLAGVAAYMLNRELRARLVAHERLRRTVDELERARFAAEESSRVK
                     SQFMANMSHELRTPLNAVIGYSEILLEDAKIEGRKECVADLRRINSAGQHLLEMIDDV
                     LDVSAIEVGGLELREEKVELAKLIEDCRHAIARSVNDAELRLSTALPPQPLLLLGDEK
                     RLRQIVLNLLSNAVKFTLAGGQIALAVTENGAGGLDIAVSDSGIGMKPEEIPLALEAF
                     RQLDVGLNRRYEGTGLGLPLARHLAELHGGTLHIESAPGEGTKVTFTLPASRVEKAVN
                     SQLSAVSSTEITIEG"
BASE COUNT          453 a          968 c          993 g          481 t
ORIGIN      
        1 ggacgtcatc aaggtcgagc ccgtagcggg cggcgatccc tggcgcgcca tcgccgcgcg
       61 gccggggatg ccggagacgc cgcacaactt tgcctggacg ctcgaggggc gcaacaagcg
      121 cagcatcgcg ctcgacctga aacagccgga cggccagcgc gtgatacggt gcctcgccgc
      181 cgccgccgac atcttcatca ccaatctgcc cttgcgtgtc cggtccgccc tcggcatcgc
      241 ctatgagcag cttgctccgg cgaatgagcg gctcgtctat gcgtcgttca cggcctatgg
      301 cgaatccgga atggaagcgg gcacgccggg tttcgatacc acggcctggt gggcgcggtc
      361 gggcctcatg gatcgcgtgc gcgccgacga ggagagcctg ccgccctggt cggtgccagg
      421 catgggcgat cacccctcgg cggtcgccat caccgccgcc attctgatgg cgctctatcg
      481 ccgcgagcgg acgggaaagg gcggctatgt cggctcctcg ctgatcgcga acggagcgtg
      541 ggcgaatgcg gtctatatcc aggcggcgct gtgcggcgcg cgtttccccg agcgtccgga
      601 gcgcagccgc gcggtgagcg cgctccgcaa ctactaccgc tgcgccgacg ggcgctggct
      661 gatgctgagt ctgagcgcgg cgcagcaggc gacgtcatgg ccggccttcg cgcgctgtct
      721 cgatcggccg gacctggtca gcgatccgcg cttcgccacg ccgatcgcgc gccgcgagca
      781 cgcgatggca ttgatcgcgc tgctggacgc cgtcttccgc acccgggaga gcgtcgactg
      841 gtattgccgc ctcgccgatg ccgggctgac ggcggcgctc atcagcacca tggatgaggt
      901 cgcgcgggac gagcagatgc gcgagagcgg cgccttcgtt ccgatgctgg cggcgggcgg
      961 cgccgagatg acggtgagca gcccgttctg gctcgccggc gagccgaagc gcgcggcgca
     1021 gccggcaccg gcgctcggcg cgcacaccga cgagatcctc cgcgccaccg atttctcgga
     1081 agccgagatc gcacgcctcc gcgcgcaggg ggtcattctc tgagttgtca gctgtcagct
     1141 ctcagctctc agccctcgat cgtgatctcg gtggagctga cggctgacag ctgagagttg
     1201 acggctttct cgacgcgcga tgccggcagc gtgaacgtca ccttggtgcc ttcgcccggc
     1261 gcgctttcga tatgcaatgt gccgccatgc agctcggcca gatggcgtgc cagcggcagg
     1321 ccgaggccgg tgccttcgta acggcgattg agcccgacat ccaattgccg gaaggcctcg
     1381 agggcgagcg gaatctcctc gggcttcatg ccgatgccgc tgtcgctcac ggcgatgtcg
     1441 agaccgccgg cgccgttttc cgtcacggca agggcaatct ggccgccggc cagcgtgaac
     1501 ttcacggcat tggagagaag attgagcacg atctgccgca gccgcttctc gtcgccgagc
     1561 agcagcagcg gctgtggcgg cagcgcggtc gagagccgaa gctccgcgtc gttcacgctg
     1621 cgcgcgatgg cgtgccggca atcctcgatc agcttcgcca gttcgacttt ctcttcgcgc
     1681 agctcgagtc cgccgacctc gatggcggag acgtcgagca cgtcgtcgat catctcgaga
     1741 agatgctgcc cggcgctgtt gatgcggcgg agatcggcga cgcactcctt gcggccctcg
     1801 atcttcgcgt cttcgaggag gatctcgctg tagccgatca ccgcgttgag cggggtgcgc
     1861 agctcatggc tcatattcgc catgaactgg cttttcacgc gactcgattc ctcggcggcg
     1921 aagcgcgccc tctcgagctc gtcgaccgtc cgccgcagcc gctcatgggc gacgagccgc
     1981 gcgcgcagct cgcggttgag catataggcg gccacgccgg cgagcaccag aatggccagc
     2041 gcgaccgcgg cctggacgat cgtggtgcgg cgccaatggg cgagcgcctc atccttggca
     2101 agcgcgacct gaaccaccag cggctgcttc tcgacgcgct gatatccgac gaccctgagc
     2161 cgtccgtcga cttcgtaggg catcacgaat acgcccgagg atgcccaggg cagccactcc
     2221 ttgaaaagcg ggctcgtgcc gaaattcctg ccgacatagc cgtcgaggtc gggctggcgc
     2281 aacagcatcg tgccgtcggt ggtctcgagc gcgatgatgc cgtactcgcc gacatggagc
     2341 gtcgagaaga attgctgcat gtaatcggcg ctcaaggtcg cgacgatgac gccggcaaag
     2401 ctgccgtcgg gccggtcgaa gcgccggctc acgaagacgc gtggcttgcg ctgcgcgggt
     2461 atgtccttct cgacggtaaa gtgcagcgca ttgttgttgc gcgccgcgac gaaatattcc
     2521 tcgcgcgcgg cgtcgagcgg tgcttgggca gcggtcgtcg agctcgcctg gacgcggccg
     2581 tccctgccga tgacggcgag catcgtgatc tgcggcgact ggcgcacgcg cgcggccaaa
     2641 agccggcgga tctcggccga ggcgcgccgg ccttcgggac cgatctcctc gagcacgatg
     2701 cgctgcgaga gttcgatcgt ctccatcgtc cgggcgacga attgctcgac cgaaagcgcg
     2761 aggttcgagc tcgactggta ggtgtcatcg atgacgatct ggcgcgactg ggcggccatc
     2821 cagaagaaca tccagcccag cgccagcgcg aaagcgcagg tgacgccgat gacccaaccc
     2881 gcttgggcgc gcgat
//
LOCUS       DASYNS010000119         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5330814, whole genome shotgun sequence.
ACCESSION   DASYNS010000119 DASYNS010000000
VERSION     DASYNS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5330814"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1833
                     /locus_tag="VGP50_03740"
     CDS             289..1833
                     /locus_tag="VGP50_03740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8026515.1"
                     /translation="MAIHGVSDEKRLELLFDAVIDYAIYMLDMAGNVSSWNAGAERIK
                     GYRAAEIVGRHFSIFYSEEDRRNDEPGRALAIAAAEGKYEREGWRVRKDGTRFWANVV
                     IDAIRDEKGQLVGFAKITRDVTERRDAQEALEQARVALAQSQKMEAIGQLTGGIAHDF
                     NNLLTVVSNNLDLILQHTSDAARVRRLAEGALRAAGRGAKLTQQLLAFARRQPLRPET
                     VEVNTLIGAIEAIIRRAAGEGVSVVLELGEKVSGVRLDAPQFEAALLNLVLNARDAMP
                     DGGVLSVKTEDVQVTEGGAATALGVAPGRYALVTVRDTGAGMSSEVQAHAFEPFFTTK
                     EPGKGSGLGLSQVYGFVTQSSGHVVLDSAPGRGTAILLYLPQVEGASAPAPAAQAKPR
                     RAIVLVVEDDPDVLESAVEMVRGLGYDVLTAANGIDALAVLRRNVTVDVLFSDIVMPR
                     GMNGVELAKEARRLRPGLRVLLSSGYASAILAAEHGLTGDFSFIGKPYRWPDLAEKLR
                     AGLVLH"
     gene            1913..>2359
                     /locus_tag="VGP50_03745"
     CDS             1913..>2359
                     /locus_tag="VGP50_03745"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV8026516.1"
                     /translation="MSTPDGIALFAYRWLPDSPPKAAVQIVHGLAEHGARYARLAQAL
                     TGAGYAVYASDHRGHGHTQATPADLGFFAERDGWRRLVDDVWQLNRQIAADHPGLPLF
                     LIGHSMGSFLAQQFISEHGEALAGVVLSGSDGKPAALASVGRLAARI"
BASE COUNT          367 a          800 c          820 g          372 t
ORIGIN      
        1 tgtcggcggc gcgctgatcg gcatacccgc ggtgggtgcc gctttgggcg cgagcgccgg
       61 agcggtcgtc ggcgccgcga cgaccccctc gcaggtcgat ctcggcaagc cggtctggcg
      121 ctaaacgcca ttcttctccc ccaaagggag agggtttgga ccatcctcgc cgcggggacg
      181 cgcaggccgg gccggactgc gcgcaatacg cgcttggcgc cccggtccga caatgttatc
      241 cagccgatgg atctaggggc ggccacctag gctgccgcgg aggtgtcatt ggcgattcac
      301 ggggtaagcg acgagaagcg actggagctc ctcttcgacg ccgtcatcga ctatgccatc
      361 tacatgctcg acatggccgg caatgtcagc agctggaacg ccggggccga gcgcatcaag
      421 ggctatcgcg ccgccgagat cgtcggccgg catttctcga tcttctatag cgaagaggat
      481 cggcgcaacg acgagccggg gcgagcgctc gccatcgccg ccgccgaagg caaatacgag
      541 cgcgagggct ggcgcgtccg caaggacggc acccgcttct gggcgaatgt cgtcatcgac
      601 gcgatccgcg acgagaaagg tcagctggtc ggcttcgcca agatcacgcg cgacgtcacc
      661 gagcggcgcg acgcgcagga ggcgctcgag caggcgcggg tggcactcgc gcaatcgcag
      721 aaaatggagg cgatcggtca gcttaccggc ggcatcgccc atgacttcaa caatctgctg
      781 accgtcgtca gcaacaatct cgatctcatt ctgcagcata cgagcgacgc ggcgcgcgtg
      841 cggcgcctgg cggaaggcgc gttgcgcgcg gcgggccgcg gcgcgaagtt gacgcagcag
      901 ctccttgcct tcgcgcggcg ccagccgctc cggcccgaga ccgtcgaggt caatacgctg
      961 atcggcgcga tcgaagcgat cattcgccgt gccgccggag agggcgtgtc ggtcgtgctc
     1021 gaactcggcg agaaagtgag cggcgtgcgc ctcgacgcgc cgcaattcga ggcggcgctg
     1081 ctcaatctcg tcctgaacgc gcgcgatgcc atgccggacg gcggcgtgct gagcgtgaag
     1141 accgaggatg tgcaggtgac ggaggggggc gccgccaccg cgctcggcgt cgcgcccggc
     1201 cgctacgccc tcgtcaccgt ccgcgacacc ggcgccggca tgtcgtccga agtgcaagcc
     1261 cacgccttcg agccattctt caccaccaag gaacccggca aaggcagcgg cctcggcctg
     1321 agccaggtct atggcttcgt cactcagtcg agcggccatg tggtgctcga cagcgcgccc
     1381 gggcgcggca cggcgatcct cctctatctg ccgcaggtcg aaggcgcgtc cgcaccagcg
     1441 ccggcggccc aggcgaagcc gcgtcgcgcc attgtgctgg tcgtcgagga cgatcccgac
     1501 gtgctcgaat cggcggtcga gatggtgcgc ggcctcggct atgacgtgct gaccgccgcg
     1561 aacggcatcg acgcgctggc ggtgctccgc cgcaacgtta ccgtcgacgt gctgttctcg
     1621 gacatcgtca tgccgcgcgg catgaatggc gtggagctgg cgaaagaagc gcggcggctc
     1681 cgtcccggat tgcgcgttct gctgtcgtcc ggctatgcga gcgccatcct cgcggccgag
     1741 cacgggctca ccggcgactt ctccttcatc ggcaagccct atcgctggcc cgatctcgcc
     1801 gagaagctgc gcgccggact cgtcttgcat tagcgtgcta ggctcggcgg cggctgcggg
     1861 gaggcagcca gttccaaacg cggggaccaa catgcaagcg tcgacccgaa ccttgagcac
     1921 gccggatggc atcgcgctct tcgcctatcg ctggctgccg gactcgccgc ccaaggcggc
     1981 ggttcagatc gtccatgggc tggcggagca tggcgcgcgt tatgcgcggc tcgcgcaggc
     2041 gctgaccggg gccggctacg cggtctatgc cagcgatcac cgcggccacg gccatactca
     2101 ggcgacaccg gccgatctcg gttttttcgc cgagcgtgac ggctggcggc ggctcgtcga
     2161 cgatgtctgg cagctcaatc gccagatcgc cgccgaccat ccggggctcc cgctgttcct
     2221 catcggccat tccatgggct cgttcctggc gcagcaattc atcagcgagc atggcgaggc
     2281 cttggccggc gtcgtgttgt cgggctcgga cgggaagccc gcggcgctgg catcggtcgg
     2341 gcggctcgcc gcgcgcatc
//
LOCUS       DASYNS010000120         6186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2992117, whole genome shotgun sequence.
ACCESSION   DASYNS010000120 DASYNS010000000
VERSION     DASYNS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2992117"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..423)
                     /locus_tag="VGP50_03750"
                     /pseudo
     CDS             complement(226..423)
                     /locus_tag="VGP50_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
     gene            complement(453..911)
                     /locus_tag="VGP50_03755"
     CDS             complement(453..911)
                     /locus_tag="VGP50_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8026517.1"
                     /translation="MAALLEKSETVVPNEEDAKLAAVSSRILAAASTERELRVHLADG
                     QELVLPKGVARLLSHLLTEMARGNAVTLFPIHAELTTQEAAEILNVSRPYLIHLLEEG
                     KLPYRRVGTHRRVRYQDLIDFQKRSDEQRSRALDELAAQAQELNMGYGDG"
     gene            complement(1296..2792)
                     /locus_tag="VGP50_03760"
     CDS             complement(1296..2792)
                     /locus_tag="VGP50_03760"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEV8026518.1"
                     /translation="MTERPYDRLEARFARLGALQEAAALLQWDNATLMPEGGAGARAE
                     QLATLKVVCHEIVTDPALGDVLAAAEAESDLDPWQRANLTEMRRRWAHAMAVPGALVE
                     ALSKACSACEMRWREARPQSDFKAVLPALKRVLSLVQEIAGAKAERLATSPYEALLDE
                     YEPGGSTATIDPLFAELAGFLPRLVEGALERQRQRPAPRVLPGPFPIEAQRALGLTLM
                     KRLGFDFAHGRLDQSLHPFCGGTPDDVRLTTRYEESDFSRALMGVLHETGHALYERGL
                     PEKWRRQPVGDARGMSLHESQSLLVEMQACRSREFVEFSAPLLRDVFHGSGPEWDADN
                     LYRLNTRVERSLIRVDADEVTYPAHVILRYRLERAMIAGELPLEDLPGAWNDGMRALI
                     GVAPPDDRRGCLQDIHWYDGAWGYFPTYTLGAMTAAQLFDAAKRADPGILPGIARGDF
                     APLLAWLRANVHSKGSRFSTAEIITQATGRPLDARTYERHLETRYLAA"
     gene            complement(2789..3538)
                     /locus_tag="VGP50_03765"
     CDS             complement(2789..3538)
                     /locus_tag="VGP50_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEV8026519.1"
                     /translation="MPSAPNEAAAGAPRKSIFWPSFFALGALVVLLGLGTWQVERLIW
                     KEGLIAARQVAVAAPPVALPSSLAAAQALEYHHVGVTGRFLNDREIYLGATSEDGRPG
                     YQVITPLLLPDGRVLLVERGFVPQERKAPEKRAQGELAGDVTVAGLLRLPPAGRPAWF
                     VPDNDAARNYWFYVDIPAMAAAARVENVLPFYVDADATPNPGGLPEGGQTRLDLPNNH
                     LQYAITWYALAAGLIAVYVVFIRRRLAEGAP"
     gene            complement(3519..4328)
                     /locus_tag="VGP50_03770"
     CDS             complement(3519..4328)
                     /locus_tag="VGP50_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEV8026520.1"
                     /translation="MSTIHGQPKHPYHLVDPSPWPIIGAFFAGIMFGGTVLYMHGGTL
                     WYVAPGVLGVLAIMFLWWRDVIIEAVYKNYHTPVVQIGLRYGMALFIASEVMFFAAFF
                     WAFFTSSLFPVGGVWPPKGIHPFNPWELPFVNTLILLTSGTTVTWAHHALLENDRKGL
                     IAGLGITVLLGIAFTSLQAYEYSHAAFGFKDGIYPSTFFMATGFHGAHVIIGTTFLLV
                     NFFRAIAGQFKPDHHFGFEAAAWYWHFVDVVWLFLFICIYWWGSGGAVGAE"
     gene            complement(4367..4954)
                     /locus_tag="VGP50_03775"
     CDS             complement(4367..4954)
                     /locus_tag="VGP50_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEV8026521.1"
                     /translation="MSPRPRNRAIVAALLFVVGAMLGLTAASVPLYRLFCQVTGYGGT
                     TQRAASVPAREVEGRLITVRFDASTAPGLDWEFRPLQAAVRVHPGEEKVIAYRAVNHA
                     KTPVTGTATFNVTPSKAGIYFDKLQCFCFTRQHLAPGESADLTVSFFVDPDIASDPAT
                     ADVDTITLSYTMFRAKDGETPVRSSALPAQTPPAN"
     gene            complement(5083..5988)
                     /gene="cyoE"
                     /locus_tag="VGP50_03780"
     CDS             complement(5083..5988)
                     /gene="cyoE"
                     /locus_tag="VGP50_03780"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEV8026522.1"
                     /translation="MTDLSTSPVKTDVAPIPAVADYVALLKPRVMSLVVFTGFVGLYL
                     APGHLHPVLAGIAMLCIAVGAGAAGAINMWYDRDIDALMERTKRRPLPAGRMAPGEAL
                     GFGSVLAVASVLVMGLGVNWAAAALLALTIAFYVGVYTMWLKRRTPQNIVIGGAAGAF
                     PPVVGWAAVTGDVGLPALALFALVFFWTPPHFWALALYRARDYAKAGVPMLPVVAGAR
                     ETKKQMLVYALLLWPAALAPALLGVAGWVYGAAALGLNALFTLAALRVWRDPTDASAK
                     RMFAFSILYLFLLFALLLVDGRAVS"
BASE COUNT         1110 a         2025 c         2095 g          956 t
ORIGIN      
        1 gaagtcgatc ttacggtctc tgaacaccgc actctccgag cctccgcgag cccgtaaatt
       61 acaatgcgat atcattgccc agagccaaag ttaacgctta tgaccctgac cctctccccg
      121 aggggagagg ggatttgtgt agacctatcg cgattggcgc accgttcgca tcgtttctct
      181 tcttcctctg cgtctctgtg cctctgcggt caatcttcct tgcatctacg ccgcgagata
      241 ccgcgtctcg aggtggcgct cgtaagtgcg ggcatcgagc ggacgccccg tcgcttgcgt
      301 gatgatctcc gccgtcgaga agcgcgagcc cttggcatgg acattggcgc gcagccaggc
      361 gaggagcggc gcgaaatcgc cgcgcgcgat gccgggatcg gcgcgctttg ccgcatcgaa
      421 gagctgcgcc gccgtcgaag acgaccgaga acctagccat ctccatagcc catgttcagc
      481 tcctgggctt gtgcggcgag ttcatccaat gcccgtgagc gctgttcgtc acttcgcttt
      541 tggaaatcga tgagatcctg gtagcgcacg cgacgatgcg taccgactct tcgataagga
      601 agctttcctt cctccagcaa gtggatgagg tacggccgcg agacgttcag gatttcggcc
      661 gcctcctgcg tcgtcagttc cgcatggatc gggaataggg taaccgcgtt tccccgggcc
      721 atctcggtca gaaggtgaga gagcaagcga gcgactcctt tcgggaggac gagttcctgc
      781 ccgtcggcca agtgcactct cagttcccgc tcggtcgatg cggcagccaa gatgcggctc
      841 gacaccgctg ccagtttcgc gtcttcctcg tttggaacga ctgtctctga tttttccaaa
      901 agcgccgcca tgggttgggc tccccgtgcc gatgcccaag actacaagga cttggaggca
      961 aacgcaataa ccgaaacaat cgaaattatc gaaacaatcg aaattatcga aagacatggt
     1021 taagtacttg attcgtggcg catacctgga cgtcgggttt gcgccagtgg acgtccgagt
     1081 cggcgagaag gacggcgcgc acgcaccttg ctcctaccgg ccgtctttaa ctcgtgcatc
     1141 gaaaggaaga ttgaccacag aggcacagag acacagagga gaaggacgcg gcgcgcgcag
     1201 cgcgccaaac cttcttctca tagttggcgc accgttcgca tcgtttctct tcttcctctg
     1261 cgtctctgtg cctctgtggt cgaccttcct tgcgcctacg ccgcgaggta ccgcgtctcg
     1321 aggtggcgct cgtaagtgcg ggcgtcgagc gggcgccccg tggcttgcgt gatgatctcc
     1381 gccgtcgaga agcgcgaccc cttgctatgc acattggcgc gcagccaggc gaggagcggc
     1441 gcgaaatcgc cgcgcgcgat gccgggcagg atgccggggt cggcgcgctt cgccgcgtcg
     1501 aagagttgcg ccgccgtcat cgcgcccagc gtgtaggtcg ggaaatagcc ccaggcgccg
     1561 tcgtaccagt ggatgtcctg gaggcagccg cggcggtcgt cgggcggcgc cacgccgatg
     1621 agcgcgcgca tgccgtcgtt ccacgcgccc ggcaggtcct cgagcggcag ctcgccggcg
     1681 atcatcgcgc gctcgagccg gtagcgcagg atgacatgcg ccggataggt cacctcgtcg
     1741 gcatcgacgc ggatcaggct gcgctcgacg cgcgtattga ggcgatagag gttgtccgcg
     1801 tcccattccg ggccgctgcc gtgaaagacg tcgcgcaaca gcggcgctga gaattcgacg
     1861 aattcgcgcg agcggcaggc ctgcatctcc accagcagcg actggctctc atggaggctc
     1921 atgccgcgcg catcaccgac cggctgacgc cgccatttct cgggcaggcc gcgctcatag
     1981 agcgcatgtc ccgtctcgtg cagcacgccc atgagggcgc ggctgaaatc gctctcttcg
     2041 tagcgcgtgg tgaggcggac gtcgtcgggc gtgccgccgc agaacgggtg caggctctga
     2101 tcgagccggc catgcgcgaa atcgaagccc agccgcttca tcagcgtcag gccgagcgcg
     2161 cgctgcgcct cgatcgggaa cgggccgggc agcacccgcg gcgccgggcg ctgccgttgg
     2221 cgctcgagcg cgccctcgac gaggcgcggc aggaaccccg ccagctccgc gaagagcgga
     2281 tcgatcgtcg cggtgctgcc gccgggctcg tattcgtcga gcagcgcctc ataagggctc
     2341 gtggccaggc gctcggcctt ggccccagcg atctcctgga cgagagacag cacccgcttc
     2401 agcgccggca gcaccgcctt gaagtcgctc tggggccgcg cctcgcgcca gcgcatctcg
     2461 caggcggagc aagctttcga gagcgcttcc accagcgccc cggggacggc catcgcatgc
     2521 gcccaacgcc gccgcatctc cgtcaggttg gcgcgctgcc agggatcgag atcgctctcc
     2581 gcctccgccg cggcgagcac gtcgccgagc gccggatcgg tgacgatctc gtggcacacc
     2641 actttcagtg tcgcgagctg ctcggcgcgg gcgccggccc cgccctcggg catgagggtg
     2701 gcgttgtccc attgcagcaa agcggccgct tcctggagcg cgcccagccg ggcgaagcgc
     2761 gcctcgaggc ggtcataggg gcgctcggtc atggtgctcc ttcagcaaga cggcggcgga
     2821 tgaagacgac atagacggcg atcagcccgg ccgccagcgc gtaccaggtg atggcatatt
     2881 gcagatggtt gttgggcagg tcgagccggg tctgtccgcc ctccggcaag ccgccgggat
     2941 tgggcgtcgc atcggcatcg acatagaagg gcagcacgtt ttcgacccgc gccgctgccg
     3001 ccatggcggg aatgtcgaca tagaaccagt aattccgcgc ggcgtcgttg tcgggcacga
     3061 accaggccgg ccgccccgcc ggcggaaggc gcagcaggcc cgcgactgtg acgtcgcccg
     3121 cgagttcgcc ctgggcgcgc ttctccggcg ctttgcgctc ttgcggcacg aagccgcgct
     3181 ccaccagcag gactcggccg tcgggcaaca gcagcggcgt gatcacctga tagccggggc
     3241 ggccgtcctc ggatgtcgcg ccgagataga tctcgcgatc attgagaaac cggccggtga
     3301 cgccgacatg gtggtattcg agggcctgcg cggcggcgag cgaggagggc agcgccaccg
     3361 gcggcgcggc gaccgccacc tggcgcgccg cgatcaggcc ctctttccag atgagacgct
     3421 cgacctgcca ggtgccgagg ccgagcagca cgacgagcgc gcccagggca aagaagctcg
     3481 gccagaagat ggattttcga ggcgctcccg ccgccgcctc attcggcgcc gacggcaccg
     3541 ccgctgcccc accaatagat gcagatgaag aggaacagcc agacgacgtc gacgaaatgc
     3601 cagtaccacg ccgccgcctc gaagccgaaa tggtggtcgg gcttgaactg gccggcaatc
     3661 gcgcggaaga aattcaccag gaggaaggtc gtgccgatga tgacatgcgc gccgtggaag
     3721 cccgtcgcca tgaagaaggt cgagggatag atgccgtctt tgaagccgaa ggcggcatgg
     3781 ctgtactcat aggcctggag cgaggtgaag gcgatgccga gaagcacggt gatgccgaga
     3841 cccgcgatca accccttgcg gtcgttctcg agcagcgcgt gatgcgccca ggtcacggtg
     3901 gtgcccgagg tcaggaggat cagcgtgttg acgaagggga gctcccaggg attgaaggga
     3961 tggatcccct tgggcggcca gacgccgccg accgggaaca ggctcgaggt gaagaacgcc
     4021 cagaagaagg cggcgaagaa catcacctcc gacgcgatga agagcgccat gccgtagcgc
     4081 agcccgatct gcaccaccgg cgtgtgatag ttcttgtaga ccgcctcgat gatcacgtcg
     4141 cgccaccaca agaacatgat ggcgagaacg ccgagcacgc ccggcgcgac gtaccagagc
     4201 gtgccgccat gcatatagag gacggtgccg ccgaacatga tgccggcgaa gaacgcgccg
     4261 atgatcggcc aggggctggg atcgaccaga tggtagggat gcttgggctg gccgtggatt
     4321 gtgctcatcg tctggctctc tgcaaaagga tcggggagcg tggccgtcaa ttcgccggcg
     4381 gggtctgggc gggaagcgcc gaggagcgga cgggcgtctc gccatccttg gcgcggaaca
     4441 tcgtgtagga gagtgtgatg gtgtcgacat cggcggtcgc gggatcgctg gcgatgtcgg
     4501 gatcgacgaa gaacgacacg gtgaggtcgg cgctttcgcc cggcgccaga tgctggcgcg
     4561 tgaagcagaa gcattgcagc ttgtcgaaat agatgccggc cttgctcggc gtcacgttga
     4621 aggtcgccgt gcccgtcacc ggcgtcttgg cgtggttgac ggcgcgatag gcgatcactt
     4681 tctcctcgcc gggatggacg cgcaccgccg cctgcagcgg gcggaattcc cagtcgagac
     4741 cgggcgcggt ggaggcgtcg aagcgcacgg tgatcagccg gccctcgacc tcgcgggcgg
     4801 gcacgctcgc ggcgcgctgc gtcgttccgc catagcctgt cacttggcag aacaagcggt
     4861 agagcggcac cgacgccgcg gtcaggccga gcatggcgcc gacgacgaag agcaacgccg
     4921 cgacgatcgc gcggttgcgg ggacggggcg acatcagaag ccccccatct tgaccagcgt
     4981 catgacgaag aagagcgcca cgacgcccaa aagcacccag agaagcgcca ggttcttggc
     5041 tcgcagtcgc cgttccctgt cgtcagccat cgcccgcatc gctcagctca ccgccctgcc
     5101 gtcgaccagc agcagcgcga acagcaggaa gagataaagg atggagaagg cgaacatgcg
     5161 cttggcgctc gcatccgtcg gatcgcgcca cacgcggagc gcggccagcg tgaagagcgc
     5221 gttgaggccg agcgcggccg cgccatagac ccagccggca acgccgagca gcgccggcgc
     5281 cagggccgcc ggccacagca gcagcgcata gaccagcatc tgcttcttgg tctcgcgcgc
     5341 gcccgccacc accggcagca tcggcacgcc ggccttggca tagtcgcgcg cgcggtagag
     5401 ggcgagcgcc cagaaatgcg gcggcgtcca gaagaagacg agcgcgaaga gcgcgagcgc
     5461 gggcaaaccc acatcgcccg tcaccgccgc ccagccgacg accggcggaa aagcgccggc
     5521 ggcgccgccg atgacgatgt tctgcggcgt gcgccgcttg agccacatcg tgtagacgcc
     5581 gacatagaag gcgatggtga gcgcgagcag cgccgccgcc gcccagttga cgccgagacc
     5641 catcaccagc acggacgcga ccgcgagcac cgagccgaat ccgagcgcct cgcccggcgc
     5701 catgcggccg gccggcagcg gccgccgctt ggtgcgctcc atcagcgcat cgatgtcgcg
     5761 gtcataccac atgttgatgg cgccggccgc gcccgcgccg acggcgatgc acagcatcgc
     5821 gatgcccgcc agcaccggat gaagatggcc cggcgccaga tagagcccga cgaagccggt
     5881 gaacaccacg agcgacatca cccgcggctt cagcagcgcg acataatcgg cgaccgccgg
     5941 gatcggcgcg acgtcagtct tgacgggcga tgtcgagaga tcggtcatgt atcacccccc
     6001 gctgcgcgct gtcccccgtc gcgctcgaat cccctccccc cttgaggggg agggctaggg
     6061 agggtggtcg cccaagccat tgctcgccct cagtgtccgg cgcccgcggc gatatgcggg
     6121 agttcctcat aagtatggaa cggcggcggc gacggcaggg tccattcgag cgtggtggcg
     6181 cccgag
//
LOCUS       DASYNS010000121         3004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3771677, whole genome shotgun sequence.
ACCESSION   DASYNS010000121 DASYNS010000000
VERSION     DASYNS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3771677"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /gene="kdpA"
                     /locus_tag="VGP50_03785"
     CDS             <1..1377
                     /gene="kdpA"
                     /locus_tag="VGP50_03785"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEV8026523.1"
                     /translation="SYVPETTMSYLTQMVGLTVHNFVSAATGIALALALIRGFVRREA
                     KTIGNFWVDLTRATLYILLPISVVVALVFMWQGMPQNLDAYADATTLEGVKQTIAQGP
                     VASQEAIKLFGTNGGGFFNANSAHPYENPTPLTNFITLLLIFAIGAGLTNVLGRMVKD
                     ERQGWAIFAAMGILFLAGVSTAYWAEAAGNPAFAALNIDSAPSELQAGGNMEGKEARF
                     GIADSALFATVTTDTSCGAVNSMHDSFTPLGGMVPLVNMELGEVIFGGVGSGLYGMLA
                     FAIVAMFVAGLMVGRTPEYLGKKLEAKEVKMTILALLSLPLSMLGWTALASVLPAGLA
                     GIANTGPHGFSEILYAFSSATANNGSAFAGISANTLFYNTTLAGAMLIGRFIFVIPLL
                     AVAGSLAQKKLLAPSAGTFPTTSPLFVGLLVGVVVIIGGLTYFPAISLGPVVEHVAMN
                     GGTLYPGP"
     gene            1409..>3004
                     /gene="kdpB"
                     /locus_tag="VGP50_03790"
     CDS             1409..>3004
                     /gene="kdpB"
                     /locus_tag="VGP50_03790"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEV8026524.1"
                     /translation="MTNVSSTVPQPSRTGLYKRQVQPLSLLDRSLLVPAIGASFRKLN
                     PRTLAKNPVMFVVEIVSVLTTIFFLRDLFAGAGTVIGTNALFSGQITVWLWFTVLFAN
                     FAEAVAEGRGKAQADTLRRMRTETKAKRLSAQQTRNFELVAATALKPGDLVLVETGDF
                     VPSDGDVIEGIASVDESAITGESAPVIRESGGDRSAVTGGTRVTSDWLKVKITAAQGS
                     TFLDRMIALVEGAVRQKTPNEIALNILLAGMTIIFVFATVSIPSFAAYAGGAVSVVVL
                     IALFVTLIPTTIGALLSAIGIAGMDRLVRFNVLAMSGRAVEAAGDVDTLLLDKTGTIT
                     LGNRQAAAFLPLAGVTERELADAAQLASLSDETPEGRSIVVLAKEKYGIRGRDMAPLN
                     AQFIPFSAHTRISGIDMEGSSIRKGAVDAVLAFIQPAMAAAAASAGRTTTGPARTVAQ
                     DPASRELNDIADRISKSGGTPLAVAKDGRLLGVVHLKDIVKGGIRERFAELRQMGIRT
                     VMITGDNPLTAAAIAAESGVDDFL"
BASE COUNT          462 a         1095 c          918 g          529 t
ORIGIN      
        1 tcttacgttc ccgagacgac catgagctac ctcacccaga tggtcgggct cacggtgcac
       61 aacttcgtct cggccgcgac cggcatcgcg ctcgcgctcg ccctcatccg cggcttcgtg
      121 cgccgcgagg ccaagaccat cggcaatttc tgggtcgatc tgacgcgcgc gacgctctac
      181 atcctgctgc cgatctcggt cgtcgtggcg ctcgtcttca tgtggcaggg catgccgcag
      241 aacctcgacg cttatgccga cgcgacgacg ctcgaaggcg tcaagcagac gatcgcgcaa
      301 gggccggtcg cttcccagga agcgatcaag cttttcggca ccaatggcgg cggcttcttt
      361 aacgccaact ccgcgcatcc ctacgagaac ccgacgccgc tcaccaactt catcacgctt
      421 ctgctgatct tcgcgatcgg cgcgggcctc accaacgtgc tcggacgcat ggtgaaggac
      481 gagcgccagg gctgggcgat ctttgccgcc atgggcatcc tgttcctcgc cggcgtctcc
      541 accgcctatt gggccgaagc ggcgggcaat cccgcttttg ccgcgctcaa catcgacagc
      601 gcgccgagcg agctccaggc cggtggcaac atggaaggca aggaggcgcg cttcggcatc
      661 gccgattcgg cgctgttcgc caccgtcacc accgacacgt cctgcggcgc ggtcaattcc
      721 atgcatgaca gtttcacgcc gctcggcggc atggtgccgc tggtgaacat ggagctcggc
      781 gaggtcatct tcggcggcgt cggctccggc ctctatggca tgctcgcttt cgcgatcgtc
      841 gccatgttcg tggccggcct gatggtcggg cgcacgcccg aatatctcgg caagaagctc
      901 gaagcgaagg aggtcaagat gaccatcctg gcgctcttga gcctgccgct ctcgatgctg
      961 ggctggacgg cgctcgcctc cgttctgccc gcgggccttg cgggcatcgc caacaccggt
     1021 ccgcacggct tcagcgagat cctctacgcc ttctcctcgg cgacggccaa caacggtagc
     1081 gcctttgccg gcatcagcgc caacacgctc ttctacaaca cgacccttgc cggtgcgatg
     1141 ctgatcggcc gcttcatctt cgtgatcccg ctgctcgccg tcgccggctc gctggcgcag
     1201 aagaagcttc tggcgccctc ggctggcacc ttcccgacca ccagcccgct cttcgtcggc
     1261 ctcctcgtcg gcgtcgtcgt gatcatcggc ggtctcacct atttcccggc catttcgctg
     1321 ggaccggtgg tcgagcacgt cgcgatgaac ggcggcactc tttatccggg tccctgacat
     1381 ggacgatcgg acaaaaggat acgcttccat gacaaacgtc tcttcgacgg tgccgcagcc
     1441 gagcaggacc ggcctctaca agcggcaggt tcagccgctg tcgctgctcg accgctcgct
     1501 gctcgtgccg gcgattggcg cctccttccg caagctcaac ccgcggacgc tcgccaagaa
     1561 cccggtcatg ttcgtggtcg agatcgtctc ggtgctgacc accatcttct tcctgcgcga
     1621 cctcttcgcg ggcgccggca ccgtgatcgg caccaacgcg ctcttctcgg gtcagatcac
     1681 cgtctggctg tggttcaccg tgctcttcgc caatttcgca gaagccgtcg ccgaaggccg
     1741 cggcaaggcg caggccgata cgctgcggcg catgcgcacc gagaccaagg ccaagcgcct
     1801 ctcggcgcag cagacccgca acttcgagct ggtcgccgcg acggcgctga agccgggtga
     1861 cctcgtgctg gtggagaccg gcgatttcgt gccgagcgac ggcgatgtca tcgagggcat
     1921 cgcctcggtc gacgagtccg cgatcaccgg cgagtcggcg ccggtcatcc gcgagagcgg
     1981 cggcgaccgc tcggccgtga ccggcggcac gcgtgtcacc tccgactggc tcaaggtgaa
     2041 gatcaccgcc gcccagggct cgaccttcct cgaccgcatg atcgcgctgg tcgaaggcgc
     2101 ggtgcgccag aagacgccca acgagatcgc gctcaacatc ctcttggcgg gcatgaccat
     2161 catcttcgtc ttcgccacgg tgagcattcc gagcttcgcc gcctatgccg gcggcgcggt
     2221 ctctgtcgtc gtgctgatcg cgctcttcgt gacgctcatc ccgaccacca tcggcgcgct
     2281 gctctcggcc atcggcatcg ccggcatgga ccggctcgtg cgcttcaatg tgctcgcgat
     2341 gtcgggccgc gccgtcgagg ccgcgggcga tgtcgatacg ctcctcctcg acaagaccgg
     2401 gaccatcacg ctgggcaatc gccaggccgc ggctttcctg ccgctcgcgg gcgtcaccga
     2461 gcgcgagctc gccgacgcgg cgcagctcgc ctcgctctcc gacgagacgc ccgaaggccg
     2521 ctccatcgtc gtcctcgcca aggagaaata cggcatccgc ggccgcgaca tggcgccgct
     2581 caacgcgcag ttcatcccct tctcggcgca tacgcgcatc agcggcatcg acatggaagg
     2641 gtcctcgatc cgcaaaggcg cggtcgacgc cgtgctcgcc ttcattcagc cggcgatggc
     2701 ggcggccgct gcttcggccg gccgcacgac caccgggcca gcgcgcaccg tcgcccagga
     2761 tccggcctcg cgcgagctca acgacatcgc cgaccgcatc tccaagtcgg gcggcacgcc
     2821 gctggccgtc gccaaggacg gccgtctgct cggcgtcgtc catctcaagg acatcgtcaa
     2881 gggcggcatc cgcgagcgct tcgccgagct ccgccagatg ggtatccgca ccgtcatgat
     2941 caccggcgac aacccgctca ccgccgcggc catcgccgcc gaatcgggcg tcgacgattt
     3001 cctg
//
LOCUS       DASYNS010000122        15549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2679729, whole genome shotgun sequence.
ACCESSION   DASYNS010000122 DASYNS010000000
VERSION     DASYNS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 15549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15549
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2679729"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /gene="gcvT"
                     /locus_tag="VGP50_03795"
     CDS             complement(<1..665)
                     /gene="gcvT"
                     /locus_tag="VGP50_03795"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013507.1"
                     /note="catalyzes the transfer of a methylene carbon from
                     the methylamine-loaded GcvH protein to tetrahydrofolate,
                     causing the release of ammonia and the generation of
                     reduced GcvH protein; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEV8026525.1"
                     /translation="MAEDILHVTPLHDLHVRLGAKMVAFAGYRMPVQFPGGIMNEHLH
                     TRARASLFDVSHMGQIRLAGDDAAAALETLVVGDILGLAPGRMRYTLFTNERGGILDD
                     LMVTNAGDHLFVVVNAACKDDDIAHLRRHLAGRVKIVPLADRALLALQGPAAAAVMAR
                     LAPAASAMAFMTGQGMEIDGLPCFVTRSGYTGEDGFEISLPADAAERLARRLLSEPDV
                     MPAG"
     regulatory      complement(717..796)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(799..888)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1056..2417
                     /locus_tag="VGP50_03800"
     CDS             1056..2417
                     /locus_tag="VGP50_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026526.1"
                     /translation="MVFARALRRRRIAVASSLALILSVLLASTDAQAVPSFAEQTGQP
                     CAACHVGAFGPRLKQVGRDFKLYGYVASDGKPKYPPLAVMAQTSFTHTDAPQAGGAAP
                     HFGPNDNFTLDQLSLFYAGRITPEIGAFMQVTYDGVARQFAWDNNDIRHAGEAELFGQ
                     DLVWGLDANNNPTVQDIWNSTPAWGFPFASSAVAPTPAAATLIDGGLAHAVEGFGTYA
                     MWNDLVYVEAAGYTGLDRGVRNALGVVPVSGSDSVDGFIPYWRLALQHEFAGGRHYAE
                     IGTYGLSADTFPGGIKSAGSDHLIDTAIDATYQFVVDPKTVVSDMVSAHVTYIKEQID
                     LNASAPLLGTNPHDTLSTFRTDVSYSLGATWTPTVQYFQTTGSNDPVRFGTSSGSPNS
                     SGVITELAYVPWGKPDSPTKRANARLALQYVAYDRFDGATRHASDNNTIFLNLWVAFT
                     YGP"
     gene            2407..2766
                     /locus_tag="VGP50_03805"
     CDS             2407..2766
                     /locus_tag="VGP50_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEV8026527.1"
                     /translation="MVHDPRSSAPRIRHIPALALAGALAAALFSDASAAETDIEQAGQ
                     RFSQTSLSIAVGDTVHFQNHDDVTHNINVIDADGQPDDQGLQKPGDIITKRFDKAGDF
                     VVRCAIHPRMKMKIEVK"
     gene            2773..4362
                     /locus_tag="VGP50_03810"
     CDS             2773..4362
                     /locus_tag="VGP50_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV8026528.1"
                     /translation="MSIRLKVLLACIGFAAIIVALGLFSRQQQQEMASLAEHLYDNTV
                     GVDYAHKVETGFVRFATTHRDVALDAAAQVQLKSMLDNLDVVIERALSDTSREAAKAI
                     RAKLILLRDQSAAPAASDLDATDVALTKLVQKYSADGFAFRSRSDDVVMQSDQWLIVG
                     VGVALALAVVIALWLGQVIVPPLNRAVAAAMAIAQGRLDNTIAATGRSETAKLLQALA
                     TMQTALAESRRRVDAARAAEIEEQARREKRQSAVERHIAAFDKLVRPTLDTLASAATE
                     MRATSETMSSTATRTSEKASAVAAASEQATANVKTVANAAEQLAASIHEIARQVAHSA
                     GIAGKAVQEAARTNSMVEGLARAAQKIGEVVNLIQNIASQTNLLALNATIEAARAGDA
                     GRGFAVVATEVKALANQTANATDDISDQIAEIQSATKAAVEAIAGIGATIREINHIAT
                     TVAAAVEEQGASTQDITYSTLQSAEGVNDVSANITGVSQGASHTGAAAVQVKSAADEL
                     GRHAEILRSEIDQFLHAIRAA"
     gene            complement(4369..4800)
                     /locus_tag="VGP50_03815"
     CDS             complement(4369..4800)
                     /locus_tag="VGP50_03815"
                     /inference="COORDINATES: protein motif:HMM:NF039957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6010 family protein"
                     /protein_id="HEV8026529.1"
                     /translation="MHVPAILEAGIVDVVSPIAVALLYIAASSLLAEPQRRNFNAVMI
                     AGAGAAYLNGGFGGWEFGFTAVLTYLAYRGLGSYRFIAIGWLLHSGWDVLHDLYGNPI
                     VPFAPTSSLGCAICDPVIALWCFAGAPSVFDLLRRSRFFAR"
     gene            complement(4812..5459)
                     /locus_tag="VGP50_03820"
     CDS             complement(4812..5459)
                     /locus_tag="VGP50_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8026530.1"
                     /translation="MEKASATRERILRHGLMLLSQAGLQGVTLGVLADRVGMSKSGLF
                     AHFRSIEEVQIALLRHTAEIAGQNVVAPAMRATEGLGRLEALVRHWLGWSGKAGLGGG
                     CPIAASMFEYDDVEGPVRDEALALERRWRALLASLVRQAIDSGELRPGLDVDQFVWEL
                     CGIYLSHHASLRFVRDVAADRRARLAVDALIERACASPVKRGARGSRRRKAKPKR"
     gene            5641..6705
                     /locus_tag="VGP50_03825"
     CDS             5641..6705
                     /locus_tag="VGP50_03825"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEV8026531.1"
                     /translation="MAISPYFADRPAAALAMIGITAAVAIAMLAAWIGFATGAPDRAA
                     ATDVRPRVDADVEIPGEPIQPIPLDLAIDLRTAELGRRLFNDPRLSHRGTISCASCHA
                     AEHDFAGDAPATEEPPRRNATLDVPTIRNSWFSIAQFWDGRAATLEAQVDFPLQSPDE
                     MASSWPEVIGRLRADPDYLAAARAIGKEPLSPELVRASIAEYERSVVSSGSRFDAFLR
                     GQGDALSADEKAGYALFKQYGCSACHQGVAVGGNLFQRLGLFGDYFHDRGGPITRADL
                     GRYNVTGDESDRYVFKVPSLREAALLPPYFHDGSVATLEAAIATMARYQLGRKIPADD
                     IRLIAAFLRSLPGTTAEARP"
     gene            6702..8702
                     /locus_tag="VGP50_03830"
     CDS             6702..8702
                     /locus_tag="VGP50_03830"
                     /inference="COORDINATES: protein motif:HMM:NF039312.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026532.1"
                     /translation="MNGRRLGWIGTACACAAIAVLSLLLLASRPPDRESGLKIVTDLR
                     NIRETEERLSRLALEARIGRLDTYDPIVAAERRIDRLTVETEDALDAAGYPGRDELER
                     YKADVAERRVLVARFESADAIFRNARAYFPLLSNRVLRAAQRAHASETVAVVADWEQV
                     VFLYWAGKARDEDLAAVEQRLRAAAARNSAMAPDLKALLTHGRTFIDAYRAAGDLLVD
                     ILDAGRGDRPNAVYRAYIERRGALDERAARIADALYVAAFLSIVGIVVSFLRLSRATT
                     RLNAANHRLETQAAELARAQRIAGVGSYTRDFIHNTGTMSRQCRDILGVSEDYALNDA
                     GLLALVHPDDRERVTEAWQTLHRGVEHLRIEHRILRPDRGVRWIHGEAEVTRDAAAAA
                     TGIEGTLHDITDLRVAQAREVELERQLLHSQRLEALGTLAGGIAHDLNNTLVPICSLT
                     GLVAKRLPEHSADRENLDVVLTAGRRARDLVKQILMFSRQGEASKETFNLSTVVREAL
                     RLMRPSILANIAFVERIGETPRLFGDSGQLFQVVINLVTNASQAIGLKPGRITVTVST
                     EPAASGPNQVVLSVADTGGGIDPAHLPRIFQPFFTTKPVNEGTGLGLSVVHGIITGHG
                     GRIDVSSTPGEGTIFTVSLPAIAAVPSAETVDATAVPLLRAS"
     gene            complement(8788..9567)
                     /locus_tag="VGP50_03835"
     CDS             complement(8788..9567)
                     /locus_tag="VGP50_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEV8026533.1"
                     /translation="MRLENKVAIVTGAASGIGKEIAALFVKEGAKVAIADLNQKGADA
                     TAAELGGPKKAVGIAVDVTNEAQVDACIAKTVDAFGQLDILISNAGIQIVAPIEEFEF
                     AKWKQLLAIHLDGAFLTTRAALRQMYRRGKGGSIIYMGSVHSKEASVLKAPYVTAKHG
                     LIGLAKVVAKEGAKHGVRANVICPGFVRTPLVDKQIPEQAKELGISEQDVIKNVMLKE
                     TVDGEFTTVDDVAQTALFLASFPTNALTGQSIVVSHGWFMQ"
     gene            complement(9584..10321)
                     /locus_tag="VGP50_03840"
     CDS             complement(9584..10321)
                     /locus_tag="VGP50_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HEV8026534.1"
                     /translation="MKETDVRERAFSMPLTSPAYPPGPYRFRQREYMIIAYRTDLEKL
                     RALVPEPLQVDEPIVKYEFIRMPDSTGFGDYTETGQVIPVSFRGRKGGYTHCMFLNDD
                     PPIAGGRELWGFPKKLANPSLRAEIDMMVGTLDYGPVRVATGTMGYKHKTADLAAVKA
                     SLEMPNFLLKIIPHVDGTPRICELVEYHLEDITMIGAWTGPGALDLHAHALAPVAELP
                     VLEIISATHIVADLTLGLGKVIYDYLR"
     gene            complement(10358..11635)
                     /locus_tag="VGP50_03845"
     CDS             complement(10358..11635)
                     /locus_tag="VGP50_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV8026535.1"
                     /translation="MDDKISKSPRRERTQLRPPFDCIALVLQGGGALGAYQGGVYEGL
                     AKAGIHPNWIGGISIGAVNAAIIAGNPPEARIEKLRQFWELITSLPQPWPSATMPGGG
                     AAGDGGKKSMFSIASSALSPMTLWANPFDFTAMLKGDAARNLVNQWSAGRVMMTGAPG
                     FFAPRPVTPWFSPEGSPEATSYYDTSQLKSTLERFADFDRINSGETRLSVGAVNVRSG
                     NFVYFDTTTHRIVPEHVMASGALPPGFPAVEIDGEEYWDGGLISNTPLRWVVDHDPQK
                     DTLVFHVDLWSARGELPRNMARVTTRQKEIMFSSRTRAAASRFKEMQRLRSMLARLFG
                     KLPAELAQSAECSALRPYAEHKVWNLVHLIYRAKNYEGDTKDFEFSRQSMEDHWRAGY
                     HDTVRTLRHPEVLERPKSADGVFIFDVAEDGRE"
     gene            complement(12106..12693)
                     /locus_tag="VGP50_03850"
     CDS             complement(12106..12693)
                     /locus_tag="VGP50_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026536.1"
                     /translation="MHRFLRRIVVLRPSAAAPATRVLARAAAASLIALLAGCSWFGGG
                     DKKDSGCPTTFIAPDLDALTLMRPGGGTTPADIRFGVKLFSANSTCTGEKVGIRTDTS
                     MSFVVARNDPDLKQGQFTYFVAVADAQQNVLAKQDFTLQVEFGPRQNQIRISDEISEH
                     LPVRDAASARNYSIVVGLQLTPEQLELNRKRGQAP"
     gene            12914..13858
                     /gene="ispH"
                     /locus_tag="VGP50_03855"
                     /gene_synonym="lytB"
     CDS             12914..13858
                     /gene="ispH"
                     /locus_tag="VGP50_03855"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEV8026537.1"
                     /translation="MTKPPLTILLAGPRGFCAGVERAIQIVERALLQFGAPVYVRHEI
                     VHNRFVVEELERKGAVFVEDLAEVPAGAPVVFSAHGVPKAVPADAERRALVHLDATCP
                     LVSKVHREAEHHFAQARRIILIGHAGHPEVVGTMGQLPPGAVTLVETAEDVARLAIAP
                     GEALAYITQTTLSIEDAASIVAALKARFPDIAGPKHEDICYATTNRQRAVKAIAGRSD
                     RLLVVGALNSSNSLRLVEVAQQSGCRRAGLIARAEDIDWGFVEGARRLGLTASASAPE
                     ILVEEVVAACRERYDVTIEDVTVTREEVRFNLPRALIA"
     gene            13866..14831
                     /locus_tag="VGP50_03860"
     CDS             13866..14831
                     /locus_tag="VGP50_03860"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV8026538.1"
                     /translation="MAVYTDVSDDELRVFVADYDIGEVTSCKGIAEGVENSNYLLRTV
                     AGTFFLTLFEKRVDPADLPFFMALMEHLATHGIACPMPIKARDGVALRHLCGRPAAIV
                     TFLDGMSPRRIQPFHCAGVGGALARLHLAGADFALRRPNNLSLAGWRTLFEACRNRAH
                     EVRKGLAEEIAQELDALGRSWPAQLPEGVIHADLFPDNIFFRGEQVSGLIDFYFACSD
                     FLVYDLAICLNAWCFESDGSFNVTKARLMLASYQALRPLSAAEFNALPLLARGSSLRF
                     LLTRLYDWLNHPPGAFVRPKDPLEYLQKLRFHRGVASPRAYGLDA"
     gene            14828..15295
                     /gene="rnhA"
                     /locus_tag="VGP50_03865"
     CDS             14828..15295
                     /gene="rnhA"
                     /locus_tag="VGP50_03865"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEV8026539.1"
                     /translation="MSAKESEDAVVEIFTDGACSGNPGPGGWGAVLRYKGKEREINGS
                     ETPTTNNRMEMMAAISALEALKRASRVHIHTDSQYLRDGITRYIHAWKARGWKTADKK
                     PVKNIDLWQRLEAAIKPHQVEWFWVKGHSGHVENERADALARAAIAQAREHLL"
BASE COUNT         2542 a         5441 c         5073 g         2493 t
ORIGIN      
        1 cccgcgggca tcacgtcggg ctcgctgagg aggcggcgcg ccaaccgctc cgccgcgtcc
       61 gccggcagcg aaatctcgaa accgtcctcg ccggtgtagc ccgagcgcgt cacgaagcag
      121 ggcaggccgt cgatctccat cccctgtccc gtcatgaagg ccatggcgga agcggcgggc
      181 gcgagccgcg ccatcacggc ggccgcggca ggcccttgca acgccagaag cgcgcgatcg
      241 gcaagcggca cgatcttgac gcggccggcg aggtggcggc ggagatgcgc gatgtcgtcg
      301 tccttgcatg ccgcgttgac gacgacgaac aggtggtcgc cggcattggt gaccatgagg
      361 tcgtcgagga tgccgccgcg ctcgttggtg aagagcgtgt agcgcatccg tccgggcgcg
      421 agcccgagaa tgtcgccgac gaccagggtc tcgagcgcgg ctgccgcgtc gtcgccggcg
      481 aggcggatct ggcccatatg cgagacgtcg aacaggctgg cgcgagcgcg cgtatgcaga
      541 tgctcgttca tgatgccgcc gggaaattgc accggcatgc gatagccggc gaacgccacc
      601 atcttggcgc cgagcctcac atggaggtca tggagcggcg tgacgtgcag gatgtcttcg
      661 gccatcaggc ctctctggca acaacaggtt tcgcgcggcc ggcgagcgac cgagccccct
      721 ctgtcccagg acctgaaaga gttacctctt cggtgaggcg cgcgtctctt gcggcgcgcg
      781 cctgctttcc agagtgccct tcccccaacg gtccttctgc ctgagagttt ccggggcggt
      841 tgctccttcg gcgccgggac ggatcccggt ctctcccctc ggggtgcgaa cggcggagcc
      901 accctagcac ccggtcgttc cgatggcgag tccgactcgg acgaggactc tcgagcccgc
      961 gacggcctgc atttaacgcc ttcttagccc gagcatacta cttttgagtg cgtccggctc
     1021 cgctctgaaa aacctccgaa gccagtggtg gaacgatggt cttcgcgcgc gccttgcggc
     1081 gccgacgcat tgctgttgcc tcgagcctgg cgcttatcct ctcggtgctg ctcgcctcga
     1141 ccgatgcgca ggcggtgccg agcttcgccg agcagaccgg ccagccatgc gctgcctgtc
     1201 acgtcggcgc cttcggcccg cgcttgaagc aggtcgggcg cgacttcaag ctttatggct
     1261 atgttgcgag cgacggcaag ccgaaatatc cgccgctcgc cgtcatggcg cagacctcgt
     1321 tcacgcacac cgacgcgccc caggcgggcg gcgccgcgcc gcatttcggc cccaacgaca
     1381 atttcaccct cgaccagctc tcgctcttct acgccggccg catcacgccc gagatcggcg
     1441 ccttcatgca ggtcacctat gacggcgtgg ccaggcagtt cgcctgggac aacaacgaca
     1501 ttcgccacgc cggcgaggcc gagctgttcg gccaggatct cgtctggggc ctcgacgcca
     1561 acaacaaccc gaccgtccag gacatctgga actcgacccc ggcctggggc ttcccgttcg
     1621 cctcctcggc ggtggcgccg acgccggcgg cggcgacgct catcgatggc ggcctcgcgc
     1681 acgccgtcga gggcttcggc acctacgcga tgtggaatga tctcgtctac gtcgaggccg
     1741 ccggctatac ggggctcgac cgcggcgtcc ggaacgcgct cggcgtcgtg ccggtgagcg
     1801 gcagcgattc cgtcgacggc ttcatcccct attggcgcct ggcgctccag cacgaattcg
     1861 ccggcggccg gcactacgcc gagatcggca cctacggcct ctccgccgac acctttcccg
     1921 gcggcatcaa atcggcgggg agcgaccacc tcatcgacac cgctatcgac gccacctacc
     1981 agttcgtcgt cgatccgaag accgtggttt ccgacatggt ctcggcgcac gtcacctaca
     2041 tcaaggagca gatcgatctc aatgcgagcg cgccgctcct cggcaccaac ccgcatgaca
     2101 ccctctcgac cttccgcacc gacgtctcct attcgctcgg cgccacctgg acgccgaccg
     2161 tccagtattt ccagaccacg ggctcgaacg accccgtgcg tttcgggacg tcgagcggca
     2221 gccccaacag ctcgggcgtg atcaccgagc tcgcctacgt cccctggggc aagcccgact
     2281 cgccgacgaa gcgcgccaat gcgcggctcg cgctgcaata cgtcgcctat gaccgcttcg
     2341 acggcgcgac gcggcacgcc tccgacaaca acacgatctt cctcaacctc tgggtggcct
     2401 tcacctatgg tccatgatcc ccgatcttcc gcgccccgca tccggcacat tccggcgcta
     2461 gcgcttgccg gcgcgctcgc cgccgcgctt ttctccgacg cctctgcggc ggagaccgac
     2521 atcgagcagg ccggccagcg cttcagccag acctccctct cgatcgccgt cggcgatacc
     2581 gtgcatttcc agaaccatga cgacgtcacc cacaacatca acgtcatcga cgcggacggc
     2641 cagcccgacg accagggtct gcagaagccc ggcgacatca tcaccaagcg cttcgacaag
     2701 gccggcgact tcgtcgtgcg ctgtgccatc catccgcgca tgaagatgaa gatcgaggtc
     2761 aaatagggcg ccatgtccat ccgtctcaag gtcctgctcg cctgtatcgg cttcgccgcc
     2821 atcatcgtcg ccctcggcct cttcagccga cagcagcagc aggagatggc gagcctggcg
     2881 gagcacctct acgacaacac cgtgggcgtc gactacgcgc acaaggtgga gaccggtttc
     2941 gtgcgcttcg ccaccacgca tcgcgacgtg gcgctcgacg cggcggcgca ggttcagctc
     3001 aagagcatgc tcgacaacct cgacgtggtc atcgagcgcg ccctcagcga cacgtcgcgc
     3061 gaggccgcca aggcgatccg cgccaagctc atcctgctcc gcgaccagtc cgcggcaccg
     3121 gcggcctccg acctcgacgc caccgacgtc gcgctgacga agctcgtgca gaaatactcc
     3181 gccgacggct ttgccttccg ctcgcgctcc gacgacgtcg tgatgcagag cgaccaatgg
     3241 ctcatcgtcg gggtcggcgt ggcgctggcg ctggcggtgg tcatcgcgct ctggctcggc
     3301 caggtgatcg tgccgccctt gaaccgcgcc gtcgcggccg ccatggcgat cgcccagggc
     3361 cgcctcgaca acaccatcgc tgccacgggg cggagcgaga ccgcgaagct cctccaggcg
     3421 ctcgccacga tgcagaccgc gctggccgag agccgccgcc gcgtcgacgc tgcgcgcgcc
     3481 gccgagatcg aggagcaggc acggagagag aagcgccaga gcgcggtcga gcgtcacatc
     3541 gccgccttcg acaagctcgt gcgcccgacc ctcgacaccc ttgcctcggc cgcgaccgag
     3601 atgcgcgcga cgtcggagac gatgtcgagc accgccaccc gcaccagcga gaaggcgagc
     3661 gccgtcgccg ccgcgtccga gcaggccacc gccaacgtca agaccgtggc caacgccgcc
     3721 gagcagctcg ccgcgtcgat ccacgagatc gcccgccagg tcgcgcattc cgccggtatc
     3781 gccggcaagg ccgtgcagga ggccgcgcgc accaacagca tggtcgaagg cctggcacgg
     3841 gccgcgcaga agatcggcga ggtggtgaac ctcattcaga acatcgcgag ccagaccaat
     3901 ctcctggcgc tcaacgccac catcgaggcg gcgcgcgccg gcgacgccgg ccggggtttc
     3961 gccgtggtcg ccacggaagt gaaggcgctc gccaaccaga ccgccaacgc caccgacgac
     4021 atctcggacc agatcgccga gatccagagc gcgaccaagg ccgccgtcga ggccatcgcc
     4081 ggcatcggcg ccaccatccg cgagatcaac cacatcgcca ccactgtcgc cgccgccgtc
     4141 gaggagcagg gtgcctccac ccaggacatc acctacagca cgctgcaatc ggccgagggc
     4201 gtgaacgacg tctccgccaa catcacgggc gtgagccagg gtgcgagcca caccggcgcc
     4261 gccgccgtgc aggtcaaatc cgccgccgac gagctcggcc gccacgccga gatcctgcgc
     4321 agcgagatcg accagttcct ccacgccatc cgcgccgcct gaccgctcct accgcgcgaa
     4381 gaatcggctt cggcgaagaa gatcgaagac cgacggcgcg ccggcgaagc accagagagc
     4441 gatcaccgga tcgcagatgg cacagccgag cgacgaggtc ggcgcgaagg ggacgatcgg
     4501 attgccatag aggtcgtgca gcacatccca tcccgagtgg agcagccagc cgatggcgat
     4561 gaagcgatag gagccgagcc cgcgataggc gagataggtc agaacggcgg tgaagccgaa
     4621 ctcccagccg ccgaatccgc cattgagata agccgcgccg gcgcccgcga tcatcaccgc
     4681 gttgaagttc cgtcgctgcg gctccgccag cagcgaggac gccgcaatat agaggagcgc
     4741 gaccgcaatc ggactcacga catcgacgat gcccgcttcg aggatggctg gtacgtgcat
     4801 ggctcttctc cctatcgctt cggctttgct ttccttcgcc gcgatccccg ggcgccgcgt
     4861 ttcaccgggg acgcgcacgc gcgctcgatc aaggcgtcga cggcgagtcg cgcgcggcga
     4921 tccgcggcaa catccctgac gaagcgcagc gacgcgtggt ggctgagata gatgccgcac
     4981 agctcccaga cgaactggtc gacatcgagg ccgggacgca gctcgccgct gtcgatcgct
     5041 tgacggacga ggctcgccaa gagcgcacgc cagcggcgct cgagggcaag cgcctcgtcg
     5101 cggacgggac cctcgacatc gtcgtattcg aacatgctgg cggcgatggg acagccgccg
     5161 ccgagccccg ccttccccga ccatccgagc caatggcgca ccagcgcttc gagccggccg
     5221 aggccctccg tcgcgcgcat cgccggcgcg acgacattct gcccggcgat ctccgccgtg
     5281 tgccgcagca aggcgatctg cacctcttcg atcgagcgga aatgcgcgaa gagcccgctc
     5341 ttcgacatgc cgacgcggtc cgccagcacg ccgagcgtga ccccctggag gcccgcctgg
     5401 ctcaacagca tgagcccgtg ccggagaatc cgctcgcgcg tcgccgaggc tttttccatc
     5461 cgccgataat agcacgaccg gtcgtgctgt caatctcgag cccccgggcg gggtgcgccg
     5521 gtatggagcg gcataggtcg ttctattcat cgccggcgcg acgcggatag aggaggggtt
     5581 aagagtcgaa cgatagactc ccgccatcgg tgagcgccgc tgtcggcggc cgaggaaggc
     5641 atggctatca gcccgtattt cgcagaccgg ccggcggcgg cgcttgccat gatcggcatc
     5701 acggcggcgg tcgccatcgc catgctcgcc gcgtggatcg gcttcgccac gggcgcgccc
     5761 gatcgcgccg cggcaaccga tgtccggccg cgcgtcgacg ccgatgtgga aattcccggc
     5821 gagccgatcc agccgatacc gctcgacctc gccatcgatt tgcggacagc ggagctcggg
     5881 cgccgcctct tcaacgaccc tcggctctcc caccgcggaa cgatctcctg cgcctcctgc
     5941 catgccgccg agcacgactt cgccggcgat gcgccggcca ccgaggagcc gccgcgacgg
     6001 aacgccacgc tcgacgtccc gacgatccgc aacagctggt tcagcatcgc gcaattctgg
     6061 gacggccgcg ccgcgacgct ggaggcgcaa gtcgattttc cgctgcagag cccggacgag
     6121 atggcatcga gctggcccga ggtgatcggc cgcctgcgcg ccgatcccga ttatctcgcc
     6181 gccgcccgcg caatcggcaa ggagccgctc tcgcccgagc tcgtccgcgc cagcatcgcc
     6241 gaatatgagc gatcggtcgt ttcgtcgggc tcgcgtttcg acgctttcct gcgcgggcag
     6301 ggcgacgcgc tgagcgcgga cgagaaggcc ggctatgcgc tcttcaagca atatggctgc
     6361 tcggcatgcc atcaaggcgt ggcggtgggc ggaaacctct tccagcgcct cggactcttc
     6421 ggcgactatt ttcacgatcg cggcggcccg atcacccggg ccgatcttgg tcgctacaac
     6481 gtcacgggcg acgagtccga ccgctacgtc ttcaaggtgc cgtcgctgcg cgaggctgcg
     6541 ctcttgcctc cctatttcca tgacggctcc gttgcgacgc tggaggccgc gatcgccacc
     6601 atggcgcgct accagctcgg gcgaaagatt cctgcggacg acatccgcct catcgccgcg
     6661 ttcctgcggt cgcttccggg caccaccgcc gaggcccgcc catgaacggg cgccgcctgg
     6721 gatggatcgg gacggcatgc gcctgcgccg ccatcgccgt gctgagcctg ctgctgctcg
     6781 cgagtcggcc gcccgatcgc gagtccggcc tcaagatcgt gaccgacctc cgcaacatcc
     6841 gagagacgga ggagaggctc tcgcgcctgg cgctcgaagc gcgcatcgga cggctcgaca
     6901 cctatgaccc gatcgtcgcg gccgagcgca ggatcgatcg actgaccgtc gagaccgaag
     6961 acgccctcga cgccgccggc taccccgggc gcgacgagct cgagcgctac aaggcggatg
     7021 tcgccgagcg ccgcgtgctg gtggcgcgct tcgaatcggc ggacgcgatc ttccgcaatg
     7081 cccgcgccta ttttccgctg ctgtcgaatc gcgtcctgcg cgccgcccag cgcgcccacg
     7141 cgagcgagac cgtcgcggtc gtcgccgatt gggagcaggt ggtattcctg tactgggcgg
     7201 gaaaggcgcg cgacgaggac ctcgcggcgg tcgaacagcg cctgcgcgcc gcggcggcca
     7261 ggaacagcgc gatggcgccc gatctgaagg cgttgctgac gcacgggcgc accttcatcg
     7321 atgcctaccg cgccgccggc gacctcctcg tcgacatcct cgacgccggc cgcggcgacc
     7381 ggcctaacgc ggtctatcgc gcctatatcg agcgccgcgg cgcgctcgat gagcgggccg
     7441 cgcgcatcgc cgacgcgctc tacgtcgcgg ccttcctctc gatcgtcggc atcgtggttt
     7501 ccttcctgcg cctgagccgc gccacgacgc ggctcaacgc cgccaatcac cggctcgaga
     7561 cgcaggcggc cgagctggcg cgggcgcaac ggatcgccgg cgtcggcagc tacacgcggg
     7621 atttcatcca caataccggc accatgtcgc gacagtgccg cgacattctc ggcgtgtccg
     7681 aggactacgc cctgaacgac gccggcctcc tcgcgctcgt gcatcccgac gaccgcgagc
     7741 gggtgacgga ggcctggcag acgttgcatc ggggcgtcga gcatttgcgc atcgagcatc
     7801 gcatcctgcg ccccgaccgc ggcgtgcgct ggatacatgg cgaggccgag gtcacgcgcg
     7861 acgcggcggc ggcagcgacc ggcatcgagg gcacgctgca cgatatcacc gacctccgcg
     7921 tggcgcaggc ccgcgaagtc gagctcgagc ggcagctcct gcattcgcaa aggctggagg
     7981 cgctcggcac ccttgccgga ggcatcgcgc atgatctgaa caatacgctg gtgccgatct
     8041 gttccctgac ggggctcgtg gcaaagcgcc tgccggagca cagcgcggac cgcgagaacc
     8101 tcgacgtggt gctgaccgcg ggccggcggg cacgcgacct cgtcaagcag atcctcatgt
     8161 tcagccgcca gggcgaagca agcaaagaga cgttcaatct ttcgaccgtc gtgcgcgagg
     8221 ccctgcgcct gatgcggccg agcatcctcg ccaacatcgc gttcgtcgag agaatcggcg
     8281 agacgccgcg gcttttcggc gatagcgggc agctcttcca agtcgtcatc aatctcgtca
     8341 ccaacgcctc ccaggcgatc ggcctcaagc cgggacggat cacggtaacc gtgtcgacgg
     8401 aaccggccgc ttccggcccg aaccaggtcg tcctgtcggt ggcggacacc ggaggcggca
     8461 tcgatcccgc gcatcttccg cggatattcc agccgttctt cacgaccaag cccgtcaacg
     8521 aaggcaccgg cctcggcctt tccgtcgtcc acggcatcat cacgggccat ggcggccgga
     8581 tcgacgtcag cagcacgccg ggcgaaggga cgatcttcac cgtgtccctg ccggcgatcg
     8641 ccgccgtgcc gtctgcggag acggtggatg cgaccgcggt gccgcttctc cgcgcgtcct
     8701 agcctttacg ggttttgatc ctatgcgcac cagcaccgtc gcgaccaaca gcgccggcct
     8761 ggctctggtg ccctggggac acggcgttta ctgcatgaac cagccatggc tcacgacgat
     8821 cgactgcccg gtgagggcgt tcgtcgggaa ggacgcgagg aagagcgcgg tctgcgccac
     8881 gtcatcgacc gtcgtgaact cgccgtcgac cgtctccttg agcatgacgt tcttgatcac
     8941 gtcctgctcg gagatgccga gctccttggc ctgctccggg atctgcttgt cgacgagcgg
     9001 cgtgcgcacg aagcccgggc agatgacatt ggcccggacg ccgtgcttgg cgccttcctt
     9061 ggccaccact ttggcaaggc cgatcagccc gtgcttggcg gtcacgtagg gcgccttcag
     9121 caccgacgcc tccttcgagt ggacggagcc catatagatg atgctgccgc ccttcccgcg
     9181 ccggtacatc tggcgcagcg ccgcgcgcgt cgtcaggaac gcgccgtcga gatggatggc
     9241 gaggagctgc ttccatttgg cgaattcgaa ttcctcgatc ggcgccacga tctggatgcc
     9301 ggcattggag atgaggatgt cgagctggcc gaaagcgtcg accgtcttgg cgatgcaggc
     9361 atcgacttgc gcttcattgg tgacgtcgac ggcgatgccg accgccttct tcgggccgcc
     9421 gagctcggcg gcggtggcat cggcgccctt ctgattgagg tcggcgatcg ccaccttggc
     9481 gccttccttc acgaagagcg cggcgatctc cttgccgatg ccgctggcgg cgccggtgac
     9541 gatggcgact ttgttctcga gacgcatggc tgttctcccg tggttagcgc agataatcgt
     9601 aaatgacctt gccgagtccg agggtcaggt cggcgacgat gtgcgtggcc gagatgatct
     9661 cgagcaccgg cagctcggcg accggcgcca gcgcatgggc atggagatcg agcgcgcccg
     9721 gcccggtcca ggcgccgatc atggtgatgt cctcgaggtg gtattcgacg agctcgcaga
     9781 tgcgcggcgt gccgtcgaca tggggaatga tcttgagcag gaagttcggc atctcgagcg
     9841 aggctttgac ggcggcgaga tcggccgtct tgtgcttgta ccccatggtg ccggtggcga
     9901 cgcgcacggg gccgtaatcg agcgtgccca ccatcatgtc gatctcggcg cggagactgg
     9961 gattggccag cttcttggga aagccccaga gctcgcgtcc gccggcgatc ggcgggtcgt
    10021 cgttcaggaa catgcaatgg gtgtagccgc ccttgcgccc gcggaacgag accgggatga
    10081 cctggccggt ctcggtgtag tcgccgaagc cggtcgaatc cggcatgcgg atgaactcgt
    10141 atttgacgat gggctcgtcc acctggagcg gctccggcac cagcgcgcgc agcttctcga
    10201 ggtcggtccg ataggcgatg atcatatatt cgcgctgccg gaagcgatag ggtcccggcg
    10261 gataggcggg gctggtgagc ggcatggaaa aggcgcgctc acgcacgtcg gtttctttca
    10321 tgagacctcc ttcggttggg cgaggcggcg gccgcgtcta ttcgcggccg tcttcggcaa
    10381 cgtcgaagat gaagacgccg tcggcgctct tcgggcgctc gagcacctcg ggatgccgca
    10441 acgtgcgaac ggtgtcgtgg tatccggcgc gccagtggtc ttccatgctc tggcgcgaga
    10501 attcgaaatc cttggtgtcg ccttcgtagt tcttggcgcg atagatgagg tggacgagat
    10561 tccacacctt gtgctcggca taggggcgga gcgccgagca ttccgcgctt tgcgccagct
    10621 cggccggcag cttcccgaaa agccgcgcca gcatgcttct cagccgctgc atctccttga
    10681 accggctggc cgcggcgcgg gtgcggctcg agaacatgat ctctttctgg cgcgtcgtca
    10741 cgcgcgccat gttgcgggga agctcgccgc gcgccgacca gagatcgaca tgaaagacca
    10801 gcgtgtcctt ctgcggatcg tggtcgacca cccagcgcag cggcgtgttg gagatgaggc
    10861 cgccatccca atattcttcg ccgtcgatct cgacggccgg gaatcccggc ggcagcgcgc
    10921 cgctcgccat gacatgctcc ggcacgatcc gatgcgtcgt cgtgtcgaaa tagacgaaat
    10981 tgccgctgcg cacattgacg gcgccgacgc tgagccgcgt ctcgccgctg ttgatgcggt
    11041 cgaaatcggc gaagcgctcg agcgtcgatt tgagctggct cgtgtcgtaa tagctcgtcg
    11101 cctcgggcga tccctccggc gagaaccagg gcgtgacggg gcgcggcgcg aagaaacccg
    11161 gcgcgcccgt catcatgacg cgccccgcgc tccattggtt gacgaggttg cgcgcggcat
    11221 cgcctttcag catcgctgtg aagtcgaagg gattggccca cagcgtcatc ggcgagagcg
    11281 ccgaggacgc gatcgaaaac atgctcttct tgccgccgtc gccggccgct ccgccgcccg
    11341 gcatcgtcgc cgacggccat ggctggggca ggctggtgat gagctcccag aactgccgca
    11401 gcttttcgat ccgcgcctcg ggcgggttgc cggcgatgat ggcggcgttg acggcgccga
    11461 tcgagatgcc gccgatccag ttgggatgaa tgccggcctt ggcgaggccc tcatagacgc
    11521 cgccctgata ggcgccgagg gcgccgccgc cctgcagcac cagcgcgatg cagtcgaaag
    11581 gcggacgaag ctgcgtccgc tcgcggcgcg gcgacttgct gatcttgtcg tccatgattg
    11641 agtgaggctc ctagcgccgc agcagtccga ccgcgagttt catgaatgcc cctttgccgt
    11701 cttgcgcgag cggcgaaagc gatgcgacga gcagaagagc cgatctcccc gtccgccgca
    11761 agccaccgtc tttgtcttgt cacgaacggc cgccaggcgc acgaccacca tcatctattc
    11821 cctgaatagg ggcgtcgctc cgccggaagg ctcggttcgc gaaaatccct ccccccgcct
    11881 cgcgcgatgg gatgctcatc cgcgcgtgga cgtgccaaac atcccctctc ccgcatcgcg
    11941 ggagagggag ggacccactg cgaagcttca gcgaagcagt gggagggtga gggggcgcgg
    12001 caagaagagt cgctgtgctt gacgcgcccc ctcacccccg cgcgacgccg gaggcgtcgt
    12061 cgcgtgcgcg cgtcactgcg aatcgcaccg ccaccaccgc cgcttctacg gcgcctggcc
    12121 gcgcttccgg ttgagctcga gctgctcggg ggtgagttgc aggccgacga cgatcgagta
    12181 gttcctggcc gaagcggcgt cgcgcaccgg cagatgctcg gagatctcgt cgctgatgcg
    12241 tatctggttc tgccgcggcc cgaactcgac ctggagcgtg aaatcctgct tggcgaggac
    12301 gttctgctgc gcatcggcga cggcgacgaa ataggtgaac tggccttgct tcagatccgg
    12361 atcgttgcgc gcgacgacga agctcatact cgtgtcggtg cggatgccga ccttctcgcc
    12421 ggtgcaggtg ctgttcgccg agaacagctt cacgccgaag cggatatccg caggcgtggt
    12481 gccgccgccc gggcgcatca gcgtcagcgc gtcgagatcg ggcgcgatga aggtggtggg
    12541 acagccggaa tccttcttgt cgccgccccc gaaccaggag cagccggcga gcagggcaat
    12601 gaggctcgcc gccgccgcgc gggcgagaac acgcgtcgcc ggggctgcgg ccgatggccg
    12661 cagaacgacg attcgtcgca ggaaacggtg cacgggcaag gctcgcaatc ggactcgggg
    12721 gtgaagctgc agcggctgag tcgcgcccga actgccccgc ccctccccct tccttatcgt
    12781 tgttggctgt actaacgttc cgccgcggcg gcgcgggcct gcccgctgcg gaatgcgcga
    12841 aactctattc gaccgccgag cggcatacaa gggctgcagg caagctttcc ggccgccgcg
    12901 aaggacgaga ccaatgacaa aaccgccgct gaccatcctg ctggccggtc cgcgtggctt
    12961 ctgcgccggc gtcgagcgcg ccatccagat cgtcgagcgc gcgctgcttc agttcggcgc
    13021 gccggtctat gtccgccacg agatcgtgca caaccgcttc gtcgtcgagg agctcgagcg
    13081 caagggcgcc gtcttcgtcg aggatctggc ggaggttccc gcgggcgcgc cggtcgtctt
    13141 ctcggcgcat ggcgtgccga aggcggtgcc ggccgacgcc gagcgccgcg cgctcgtcca
    13201 tctcgatgcc acctgtccgc tggtgagcaa agtgcatcgc gaggccgagc accatttcgc
    13261 tcaagcccgc cgcatcatcc tcatcggcca tgccgggcat cccgaggtcg tcggcaccat
    13321 gggccagctg ccgccgggcg ccgtgacgct ggtcgagacg gccgaggatg tggcgcgtct
    13381 cgccatcgcg cccggcgaag cgctcgccta catcacgcag acgaccttgt cgatcgagga
    13441 cgcggcgtcg atcgtcgccg cgctcaaggc gcgctttccc gatatcgccg ggccgaagca
    13501 cgaggacatc tgctacgcca ccaccaaccg gcagcgtgcc gtcaaggcga tcgccggccg
    13561 gtcggaccgg ctgctggtgg tcggcgcgct caactcgtcg aactcgttgc ggctcgtcga
    13621 ggtggcgcag cagagcggct gccgtcgcgc aggcctgatc gcgcgggccg aggacatcga
    13681 ttggggcttc gtcgaaggcg cgcggcggct cgggctcacg gccagcgcct cggcgcccga
    13741 gatcctcgtc gaggaagtcg tcgcggcctg ccgcgagcgc tacgacgtga cgatcgagga
    13801 cgtcaccgtg acccgcgaag aagtgcgctt caacctgccg cgcgcgctca tcgcgtgaga
    13861 agctgatggc cgtctatacc gacgtctccg acgacgaatt gcgcgtcttc gtcgccgact
    13921 acgacatcgg cgaagtgacc tcgtgcaaag gcatcgccga aggcgtcgag aactcgaact
    13981 acctgttgcg caccgtcgcc ggaacgttct ttctgacgct cttcgagaag cgcgtcgatc
    14041 cggccgatct gccgttcttc atggcgctga tggagcatct cgccacgcac ggcatcgcct
    14101 gtccgatgcc catcaaggcg cgcgacggcg tggcgctgcg ccacttgtgc ggccgtcccg
    14161 cggccatcgt gacctttctc gacggcatgt cgccacgccg catccagccc tttcactgcg
    14221 ccggcgtcgg cggcgcgctg gcgcgcctcc accttgcggg tgccgacttc gccctgcggc
    14281 gccccaacaa cctctcgctc gccggctggc ggaccctgtt cgaggcgtgc cgcaaccgcg
    14341 cacacgaagt gcgcaagggc ctcgccgagg agatcgccca ggagctcgat gcgctcggca
    14401 gaagctggcc ggcacagctt cccgagggcg tcatccacgc cgatctcttt ccggacaaca
    14461 tcttctttcg cggcgagcag gtctcgggcc tgatcgactt ctatttcgcc tgcagcgatt
    14521 tcctcgtcta tgacctcgcc atctgtctca acgcctggtg cttcgagagc gacggcagct
    14581 tcaacgtgac caaggcgcgt ctcatgctgg cgagctatca ggcgctgcgc ccgctcagcg
    14641 cggccgagtt caacgccttg ccgctgctgg cgcgcggcag ctcgctgcgc ttcctgctga
    14701 cgcgcctcta tgactggctc aatcatccgc cgggcgcttt cgtgcgtccc aaggacccgc
    14761 tcgaatattt gcagaagctg cgcttccatc gcggcgtcgc gagcccgcgc gcctatggcc
    14821 tcgacgcatg agcgcgaagg aaagcgagga cgccgtcgtc gagatcttca ccgacggcgc
    14881 ctgcagcggc aatcccggcc ctggcggctg gggcgcggtg ctgcgctaca agggtaaaga
    14941 gcgcgagatc aatggctccg agactccgac caccaacaac cgcatggaga tgatggccgc
    15001 gatctcggcg ctcgaggcgc tgaagcgggc gagccgcgtc cacatccaca ccgacagcca
    15061 gtatctgcgc gacggcatca cccgctacat ccatgcctgg aaggcgcgcg gctggaagac
    15121 cgccgacaag aagccggtca agaacatcga tctctggcag cggctcgagg cggcgataaa
    15181 gccgcaccag gtcgagtggt tctgggtcaa aggccattcc ggccacgtcg agaacgagcg
    15241 cgccgacgcc ctcgcccgcg ccgccatcgc ccaggcgcgg gagcaccttt tgtagctgtc
    15301 agcttcgtta gagcgcatcc cgattggaat cgatgcccac cctatcgtca tccccgcgaa
    15361 agcggagacc caggggcgag cgacgtgccg gtggcccctg ggtccccgct ttcgcgctag
    15421 ggtacgtaca gaacagccgt gggcgcgggt gcgtcaaatc gactcggcgc gttcgccaca
    15481 gattcccaaa gggcaccttt ccgtgattct tgtactggca cgctcggcaa cgggggtgcg
    15541 gcatggaac
//
LOCUS       DASYNS010000123        16451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5331195, whole genome shotgun sequence.
ACCESSION   DASYNS010000123 DASYNS010000000
VERSION     DASYNS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 16451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16451
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5331195"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VGP50_03870"
     CDS             complement(<1..219)
                     /locus_tag="VGP50_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEV8026540.1"
                     /translation="MTEVRMMAEQSAPKETEDGLLRMTAEVVAAYVSNNTLPTGQLAE
                     VISAVYKSLRALDGQTLESQSEPLKPAVA"
     gene            574..1005
                     /gene="rpiB"
                     /locus_tag="VGP50_03875"
     CDS             574..1005
                     /gene="rpiB"
                     /locus_tag="VGP50_03875"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEV8026541.1"
                     /translation="MTETVVIAADHGGFDLKSALVPELRALGHEVVDLGAMSRDAVDY
                     PDVAEKVAQAIRAGRASRGVLICGSGIGMSIAANRYREVRAALVHDGLTARLARQHND
                     ANVLVLGGRLLGPELARDCLKIFFDTAFEGGRHVARVAKLS"
     gene            1111..1572
                     /gene="nrdR"
                     /locus_tag="VGP50_03880"
     CDS             1111..1572
                     /gene="nrdR"
                     /locus_tag="VGP50_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEV8026542.1"
                     /translation="MRCPFCGCDDTQVKDSRPTEDRAAIRRRRLCPNCAARFTTFERV
                     QLRELTVIKKNAQREPFDRDKLARSIHVSLRKRPVEPERVERVINSIVRQLESSGETE
                     IPSDMIGELVMESLSQLDQVAYVRFASVYRNFREAKDFGEFVGRIRADADD"
     gene            1913..3001
                     /gene="ribD"
                     /locus_tag="VGP50_03885"
     CDS             1913..3001
                     /gene="ribD"
                     /locus_tag="VGP50_03885"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV8026543.1"
                     /translation="MRAALALARRGLGTVWPNPSVGCVLVAEGRVVGRGWTQRGGRPH
                     GETEALERAGALARGATAYVSLEPCCHWGKTPPCTDALIAAGIARAVIPIEDPDPRVS
                     GRGIAKLREAGIAVDMGLCAQEAAELNAGFFLRLRVGRPLVTLKLAATLDGRLATAAG
                     ESQWITGPVARDRGHLLRASHDVVMIGSNTVLADDPRLTCRLPGLDGQSPVRVVVDGR
                     LRVPLTARVVAEASRVPAWFITLKHGDAARRSAFQRAGVELIEVPATGDHTVDMAAAL
                     HELGTRGVTRVLVEGGATLAAVLLRAGLVDRLAWFHAPLLLGGDAVPAVAPLGIHKLA
                     DAPRFERIALEAVGEDVLETLRRAGAAS"
     gene            3213..3716
                     /locus_tag="VGP50_03890"
     CDS             3213..3716
                     /locus_tag="VGP50_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026544.1"
                     /translation="MNTARLIRLIAALCVTALLFALTAFAVEEWLVFLDWAKTAPEGK
                     WIAADAAVGLVALAWLIRRRKRAARVISREVARGIIEERLKLLRQVSYNELLKRRGET
                     HYECLPGVRGREYQVETTVLWERSKKKDNLRVMVSVDGGGVSAWKPMIDDFVRAPDGS
                     FVGKRSR"
     gene            3771..4364
                     /locus_tag="VGP50_03895"
     CDS             3771..4364
                     /locus_tag="VGP50_03895"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV8026545.1"
                     /translation="MFTGIISDVGRVRAVTPGGDTGFTIATAYDARDLVLGASVACAG
                     VCLTVTGKGEGWFTVQASAETLARSTLGSWRVGTPVNLERALRLGDELGGHILNGHVD
                     AVATILDRRPEGDSLRFVFEVPPPLERAIAPKGSVALDGVSLTVNEVEGRRFGVNIIP
                     FTRTKTTFGAAQPGDRVNLEIDVLARYVARLLGKDVA"
     gene            4361..5482
                     /gene="ribB"
                     /locus_tag="VGP50_03900"
     CDS             4361..5482
                     /gene="ribB"
                     /locus_tag="VGP50_03900"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEV8026546.1"
                     /translation="MSFVEVVSPIEDIIDDARNGRMFILVDDEGRENEGDIVVPAQMA
                     TPSAINFMAKHGRGLVCLALTRQRVEQLKLPLMPQLNSSRLQTAFTVSIEAREGVTTG
                     ISASDRARTIAVAIDAGKGPQDIVTPGHIFPLMARDGGVLERTGHTEAAVDLARLAGL
                     NASGVICEIMNDDGSMARRPELIAFAQRHGLKIATIADLIAYRRRHDRIVARTVETPF
                     RSRFGGDFKMYIYANRISNVEHIALVKGDIATPEPVLVRMHALNVLDDVLGNDLRNRG
                     GLLHAAMEAIGREGRGVVVLIRETVSNSLSDRLTSLLDHEGYGGTELRNYGVGAQILL
                     DLGVRDMILLSNTERTIVGLDGYGLAVVEQRPVARGGGS"
     gene            5482..5946
                     /locus_tag="VGP50_03905"
     CDS             5482..5946
                     /locus_tag="VGP50_03905"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV8026547.1"
                     /translation="MAEAPRIMIVEARYYAHVSDELARGAIAALDAAGASYERFTVPG
                     AFEIPAAIRYAVEAQERGARATRFDGYVALGCVIRGETTHYDYVCGESARGLQDLALA
                     HSLAIGFGILTVENEAQALARARPDRKNKGGEAANACLAMIELKRHFGLERR"
     gene            5943..6458
                     /gene="nusB"
                     /locus_tag="VGP50_03910"
     CDS             5943..6458
                     /gene="nusB"
                     /locus_tag="VGP50_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV8026548.1"
                     /translation="MTGDPPSRAPPGLRRRSAARLAAVQALYQIDLGGATPEAVIAEF
                     RFHRFGREIDGENYGEADTALFAEIVTGVVERQAELDQTISGALTPDWPLERLETVLR
                     AILRAGTFELLALREVPARVVISEYLDIAHAFFAGKEPGLVNGVLDHIAHRLRPGDLE
                     EGGGGGGPAPR"
     gene            6436..7416
                     /gene="thiL"
                     /locus_tag="VGP50_03915"
     CDS             6436..7416
                     /gene="thiL"
                     /locus_tag="VGP50_03915"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEV8026549.1"
                     /translation="MAAPHLDEFERIAKFFAPLAAPGALDLLDDVALIAGPPGEEYVL
                     KTDAIVEGVHFLPEDPPAQVAQKLLRVNLSDLAAKGSAPVGYLLMTALPPSRDEAWLA
                     AFAQGLAADQATYGIGLLGGDSVATAGPVTLSVTAIGKVAAGRAVLRRGARAGDLIFV
                     TGTLGDAALGLAVLRGGLDTLAPAHQAFLVERYRLPQPRLVPGRRLAGLVHAMMDISD
                     GLVADLGHICKASGVGALIEAARLPLSAAARAAVAAEPARLMAALGGGDDYELLFTAA
                     AAAAGAIAALSTEIGVALTRIGRIEAGAGVRVLDPSGAPMSVPVGGYRHF"
     gene            7498..7941
                     /locus_tag="VGP50_03920"
     CDS             7498..7941
                     /locus_tag="VGP50_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026550.1"
                     /translation="MAMACFRRTLLLAALTSMVLAAMPAFAVDREKGLKPPPVPGVPD
                     FVDLDPIVLPVIERNRVTRQIGLMLTLELFDGQPAQAIDEEKRLRLMDAFVRELHLIY
                     SARSTAERVVDEDIIKRRLLRIADGVLGTGVVHAILVRRLIEQRG"
     gene            8067..10166
                     /locus_tag="VGP50_03925"
     CDS             8067..10166
                     /locus_tag="VGP50_03925"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEV8026551.1"
                     /translation="MNAYWFVIAGGVVLALLYGFLTGSRVLAANAGTARMQEISAAVR
                     EGAEAYLNRQYRTILMVGVVILIILWATLGWRSALGFLVGAVLSGAAGYIGMNVSVRA
                     NVRTAQAAREGLAAGLSIAFQSGAVTGMLVVGLGLLGVSAYYFFLRATLPGDDARRFL
                     EPLVALSFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVG
                     DNVGDCAGMAADLFETYAVTIVATMLLAAIYFTPPMRDIMLMLPLVIGGVCIIASVIG
                     TYFVRLGRSGNIMAALYKGLVVSGVISAGLVAAVIAELIGFDTPLTMTTGVTTTGTGI
                     FGCCLVGLAVTGLLVWITEYYTSTAYRPVRSIALASVTGHGTNVIQGLAVSMEATALP
                     VIVICIGIIVSYLIAGLFGIAVAATTMLALAGMIVALDAYGPVTDNAGGIAEMADLPK
                     DVRKTTDALDAVGNTTKAVTKGYAIGSAGLASLVLFAAYTEDLQHYFPNVSVNFGLQN
                     PYVVVGLLIGGLLPYLFGAMGMTAVGRAAGSVVIEVRRQFKEIPGLMQGTAKPDYSRA
                     VDLLTQAAIKEMIVPSLLPVLAPVVLYLVIRAIGGQGAAFSAVGAMLLGTIVTGLFVA
                     ISMTSGGGAWDNAKKYIEEGHHGGKGSDAHKAAVTGDTVGDPYKDTAGPAVNPMIKIT
                     NIVAILLLAILASLSGS"
     gene            complement(10168..10686)
                     /locus_tag="VGP50_03930"
     CDS             complement(10168..10686)
                     /locus_tag="VGP50_03930"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEV8026552.1"
                     /translation="MIRRLPPIRALASGIAVLAVAACSNPVDQRGNLPAKDALDQITP
                     GATDKATVTRLLGSPSSVAAFEPNTWYYVSQKTKDAAFFKPQLIDQEVTAIDFDKDGV
                     VRDVRHRGLQDRQAITPNPNATPAPGREFSFLEQLIGNFGKFSGSNNPLGGAGGGGNP
                     GGGPSGPGRGGY"
     gene            10756..11364
                     /locus_tag="VGP50_03935"
     CDS             10756..11364
                     /locus_tag="VGP50_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEV8026553.1"
                     /translation="MPAAIDRAGRRTHLASRRGAMGGLLKSWHRRRERRSAMLGAYRQ
                     IVDRAREPVLFHVWGVPDTLDGRFEVLALHAFLVLHRLKREGEATAEFAQALFDTMFA
                     DLDGALREMGASDLGVGPRIKAMARGFYGRVAAYERGLGDSGDLEAALRRNLFGTIRP
                     GEAQVEAASRYLRQQVAALAAAPVEALLEGRVAFAALAGAGE"
     gene            11481..11669
                     /gene="rpmF"
                     /locus_tag="VGP50_03940"
     CDS             11481..11669
                     /gene="rpmF"
                     /locus_tag="VGP50_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV8026554.1"
                     /translation="MAVPKKKVSKSRRNMRRAHHALEAVSYAECSNCGELKRPHHVCA
                     ACGHYDGREVVAVPAETA"
     gene            11695..12750
                     /gene="plsX"
                     /locus_tag="VGP50_03945"
     CDS             11695..12750
                     /gene="plsX"
                     /locus_tag="VGP50_03945"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV8026555.1"
                     /translation="MSDRVTIALDAMGGDRAPDMVLKGASIALQRFPQVHFRIYGIES
                     HIRPLMAKLPRLAGKATLHHTDDVVTDDAKPSTALRTGRRSSMRLAIDAVAEGEADGV
                     VSAGNTGALMAMAKFALKMLPGIDRPAMASFFPTRRGESVMLDLGANVECDADNLVQF
                     AVMGDVFARTVLGLLQPTVGLLNVGSEDLKGNDAVRGASAQLRGAGLPIRFYGFIEGD
                     DIGAGTVDVVVTDGFTGNVALKVMEGTARLYGEFLRASFKNSLTARIGYLLARRSLHK
                     LRDRLDPRRHNGAVFLGLQGIAVKSHGSADALSFANAIGVAVDMKVNGFLEQIRDGVA
                     RLHALQAPVEKPQSAAL"
     gene            12752..13726
                     /locus_tag="VGP50_03950"
     CDS             12752..13726
                     /locus_tag="VGP50_03950"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEV8026556.1"
                     /translation="MMFRAQVIGCGAYLPERVVTNAELAQRLDTSDEWIVQRTGIRQR
                     HIAAPGELTSDLAFRASRDALAAAGLKGSDLDLIVLATATPDNTFPATATKLQARLGM
                     TKGAAFDVQAVCSGFVYALAVADNFIRSGQAQTALVVGAETFSRILDWEDRGTCVLFG
                     DGAGAVVLRSVASRGAATERGVLSTHLHSDGTGYDLLYVDGGPSTTGSSGFLRMEGKE
                     IFRQAVQRLAEVVDEALAANKLTARDVDWLVPHQANRRIIDSMARKLGLSGDKVVMTI
                     ERHANTSAASVPLALAEAVADGRIKPGHLVLLEAMGGGLTWAAGLLRW"
     gene            13813..14151
                     /locus_tag="VGP50_03955"
                     /pseudo
     CDS             13813..14151
                     /locus_tag="VGP50_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
     gene            14154..14612
                     /locus_tag="VGP50_03960"
     CDS             14154..14612
                     /locus_tag="VGP50_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV8026557.1"
                     /translation="MPVDLMSPPAADAAERASRRQQKSADAFRTISEVAQELEIPQHV
                     LRFWESRFAQLRPLKRAGGRRYYRPDDVVLLRRIRQCLYDQGYTIKGVQKLLREGALK
                     SEAVLRPTAAAPVAVTPAAPRVKDGGNRTHLVELRRELVELRDLLRRARR"
     gene            14653..14729
                     /locus_tag="VGP50_03965"
     tRNA            14653..14729
                     /locus_tag="VGP50_03965"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14687..14689,aa:Pro,seq:ggg)
     gene            complement(14845..>16451)
                     /locus_tag="VGP50_03970"
     CDS             complement(14845..>16451)
                     /locus_tag="VGP50_03970"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     tetratricopeptide repeat protein"
                     /protein_id="HEV8026558.1"
                     /translation="PVPSAERPDMRFVFAGHTLDTDRRELCRGSEAIAVEPQVFDLLT
                     YLLENRDHVVSKDDLIASVWGGRIVSDSTLTSRVNAARKAVGDSGDAQRLIRTIARKG
                     LRFVGTVHPQSPSEETADAVTAASREFHEAPRSALPLPDRPAIAVLPFTNMSGDPEQE
                     YFSDGISEDIITALSKLRWFFVIARNSSFIYKGKPVHMKQIAAELGVGYVVEGSVRKS
                     GDLVRITAQVNDVASGGHIWAERYDRGLADVFAVQDEITESIVAAIEPRLYAAENFRA
                     QRKPPDSLDAWDLVMRALSHFWRVTRQDNVVAQALLEKATAIDPNYGQALGVLAASHS
                     FSAHMGWADMATVMPIAERAALAAIRVDSEDAWAHNALATACLFARRFDDALAEFELA
                     LRLNPNFSMAQGYYGLTLCYCGRWQEGLVAANRALRLSPRDPFSGVYYGIAAYAQFIG
                     RNYEEAMRLARQGLRQRADFVGAHRVLTASAAMAGAAEVAAAALQELRRAQPNISLAW
                     IAKQMPIKQESEREHYIEAFRRAGLD"
BASE COUNT         2543 a         5505 c         5598 g         2805 t
ORIGIN      
        1 cgccacggcg ggcttgagcg gctcggactg cgattcaagg gtctggccat cgagcgcccg
       61 cagcgacttg tagacggcgc tgatgacctc ggcgagctgt ccggtcggca aggtattgtt
      121 gctgacatag gccgcgacga cctcggccgt catgcggagc agcccatctt ctgtttcttt
      181 cggcgcgctc tgctcggcca tcatccggac ctcagtcacg atttagaatt cgcacggcat
      241 gattgcaggg tgattggaac gtgtcaatca aaacccccgc gatagagaaa taatgaacgc
      301 tgcaatgcct gcgaagtctg ccacgtttcc tgcccgcaca tgtgggcacg cgccgcgcct
      361 agcgaagacg cgagaagtgg ccccccgata ctggcgctgc gccaccaaag tctaagattt
      421 cggcacggcg actttacggg gcgcgtgagc gactaattcc ctgaaagaga gaacggcgat
      481 acgggacggt agacgcgatg aatggtagcc cactgacaaa cgaccccgag atttggtagg
      541 atcatacgcg attccgtatc cgcgccaggt tccatgactg agacggtcgt catcgctgcc
      601 gatcacggcg gcttcgatct gaaaagtgct ctcgtgccgg agcttcgcgc gctcggtcac
      661 gaggtcgtcg atctcggcgc gatgagccgc gatgccgtcg actatccgga tgtcgcggag
      721 aaggtggcgc aagccattcg cgcggggcgc gcgagccgag gcgtgttgat atgcggtagc
      781 gggatcggca tgtcgatcgc ggccaatcgc tatcgcgagg tccgcgcggc actcgtccat
      841 gacggcttga cggcgcggct cgcgcgtcag cacaacgacg ccaacgtcct cgtgctgggc
      901 ggacgtctgc tgggcccgga gctggcgaga gactgtctca agatcttctt cgacaccgca
      961 ttcgagggcg gccgccatgt ggcgcgcgtc gccaagttgt cgtgaaccgc ggcgccgcca
     1021 tcagacctcg caacaacgcg gtggccgcgg ccgcggcgct gcgggtggcg gcgtcctgtc
     1081 attcgagctg accgcagggg gagagagagg atgcgttgtc cattctgcgg ctgcgatgat
     1141 acgcaggtga aggattcgcg accgaccgag gatcgcgcag cgatccgccg gcggcgcctt
     1201 tgccccaact gcgccgcgcg cttcaccacc ttcgagcgcg tgcagctgcg cgagctgacg
     1261 gtgatcaaga agaacgccca gcgcgagccc ttcgaccgcg acaagctcgc gcgctcgatc
     1321 catgtctcgc tgaggaagcg cccggtcgag cccgagcgcg tcgagcgcgt catcaactcg
     1381 atcgtgcgcc agctcgaaag ctccggcgag accgagatcc cgagcgacat gatcggcgag
     1441 ctcgtgatgg aatcgctctc ccagctcgat caggtcgcct atgtgcgctt cgcctcggtc
     1501 tatcgcaatt tccgcgaggc caaggatttc ggcgagttcg tcggccgcat ccgcgccgac
     1561 gcggatgact agggcgggaa atcgtggcgc agcggcgacg caccccccac cctaatgctt
     1621 cccctcgaag gggacttgtt tgcgcaccga ggctttccac ctctcccctt gaggggcagg
     1681 gctatcgcat atggggttca ccacggaggc acggaggaca cggagaagag gggttcggcg
     1741 cgcgaagcgc gccatccttt gcttctccgt gtcctccgtg cctccgtggt caatctttcc
     1801 tatcgacgtc tcggcagagt atgcgattgc cctgccttga ggggcaggaa ggggtggtgg
     1861 gtttcgtgca gctccccgac gacatcttat gattatgaac gacctccatc ccatgagggc
     1921 ggcgctggcg ctggcgcgac gcggcctggg caccgtctgg cccaatccgt cggtcggctg
     1981 cgtgctcgtc gctgagggtc gtgtcgtcgg ccgcgggtgg acacagcgcg gcgggcgccc
     2041 gcacggcgag accgaagcgc tggagcgtgc cggagcgctc gcccgcggcg ccaccgccta
     2101 tgtcagcctc gagccctgct gccattgggg caagacgccg ccctgcacgg acgcgctgat
     2161 cgccgcgggc attgcccgtg cggtgatccc gatcgaggat cccgatccgc gtgtctcggg
     2221 ccgcggcatc gccaagctgc gcgaagcggg catcgccgtc gacatgggac tctgcgccca
     2281 ggaagcggcc gagctcaatg ccggcttctt tctgcgcctt cgcgtcgggc ggccgctcgt
     2341 cacgctgaag ctcgccgcca cgctcgacgg ccgcctcgcc accgcggcgg gcgagagcca
     2401 atggatcacc ggtcccgtcg ctcgcgaccg cggccatctg ctgcgcgcca gccatgacgt
     2461 cgtgatgatc ggcagcaaca ccgtgctggc cgacgatccg cgcctcacct gccgcctccc
     2521 cggcctcgac gggcagagcc cggtgcgcgt ggtcgtcgac ggccggctgc gcgtgccgct
     2581 cacggcgcgg gtcgtcgccg aggccagtcg cgtgcctgcc tggttcatca cgctgaagca
     2641 tggcgacgcg gcgcgtcgct cggccttcca gcgcgccggc gtcgagctca tcgaagtgcc
     2701 ggcgacgggc gaccataccg tcgacatggc ggcggcgttg cacgagctcg gtacgcgcgg
     2761 cgtgacgcgc gtcctcgtcg aaggcggcgc aacgctcgcg gcggtgctgt tgcgcgccgg
     2821 gctggtcgat cgcctcgcct ggtttcatgc gcccctgctg ctcggcggcg acgcggtgcc
     2881 cgcggtcgcg ccgctcggca tccacaagct tgcggatgcg ccgcgcttcg agcgcatcgc
     2941 cctcgaggcg gtgggggaag acgtgctcga gacgctgcgg cgggccggcg cggcgagctg
     3001 aggtgaagcg caggctagtt tccaaccagc gcgcgggcga cgtccatagg cagcagcatc
     3061 acgcgttcgc cgagcggcga cagcagaaag ccgtgtcgct catagaagcc gacagcggta
     3121 attcagtgta ctgacattgt taatacattt gagcggtggc gcctcaccgg aggcgcacgt
     3181 tccattaacc gcgggcatgg caccatcagt ccatgaacac ggctcggctc atcaggctga
     3241 tcgctgccct ctgcgtcacg gcgctgttgt tcgcgctgac ggcgttcgcc gtcgaagagt
     3301 ggctcgtatt cctggactgg gcgaagaccg cgcccgaggg caagtggatc gcggccgatg
     3361 ccgccgtggg cctggtggcg ctggcctggc tcatccgccg ccgcaagcgc gccgcgcgcg
     3421 tgatcagccg cgaggtcgcg cgcggcatca tcgaggaaag gctgaagctt ctgcggcagg
     3481 tgtcgtacaa cgaattgctg aagcgtcgcg gcgagacgca ttacgaatgc ctccccggcg
     3541 ttcgcggccg ggaataccag gtcgagacca cggtgctctg ggagagatcc aagaagaagg
     3601 acaatctccg cgtcatggtg tcggtcgacg gcggcggcgt cagcgcgtgg aagccgatga
     3661 tcgacgactt cgtccgcgcg cccgacggga gcttcgtcgg caagcgctcg cgctgagcgg
     3721 cgcgtcggcg ccgtgacagg cggcggcgga agcactaagt tatccgtggc atgttcacgg
     3781 ggatcatcag cgacgtgggg cgagtgcgcg cggtcacgcc gggcggtgat accggattca
     3841 cgatcgcgac cgcatatgat gcgcgcgacc tcgttttggg cgcgtcggtc gcctgcgccg
     3901 gcgtctgcct caccgtaacg gggaaggggg agggctggtt caccgtccag gcctcggcgg
     3961 agactctggc ccgcagcacg ctcggcagct ggcgcgtcgg cacgcccgtc aatctcgagc
     4021 gcgcgctcag actgggcgac gagctcggcg ggcatattct caacgggcat gtggacgccg
     4081 tcgcgaccat cctcgaccgg cggcccgaag gcgattcgct ccgcttcgtc ttcgaggtgc
     4141 cgccgccgct cgaacgggcg atcgcgccca aaggctcggt ggcgctcgac ggcgtctcgc
     4201 tcaccgtcaa cgaagtcgag gggcgccgct tcggcgtcaa catcattccg ttcacgcgga
     4261 cgaagaccac cttcggtgcg gctcagccgg gcgaccgggt caacctcgag atcgacgtcc
     4321 tcgcccgtta tgttgcgcgc ctcttgggaa aggatgtggc atgagctttg tcgaggtcgt
     4381 ctcgcccatc gaagatatca tcgacgacgc gcgcaatggc cggatgttca tcctggtcga
     4441 tgacgagggc cgcgagaacg agggcgacat cgtcgttccc gcgcagatgg cgacgccgtc
     4501 ggcgatcaac ttcatggcca agcacggccg cggcctcgtc tgcctcgcct tgacgcggca
     4561 gcgcgtcgag cagctgaagc tgccgctcat gccgcagctg aacagctcgc gccttcagac
     4621 cgcgttcacc gtctcgatcg aggcgcgcga gggtgtcacc accggcatct cggcttccga
     4681 ccgcgcgcgc accatcgccg tcgccatcga cgccggcaaa gggccgcagg acatcgtcac
     4741 gcccggccat atcttcccgc tgatggcgcg cgacggcggc gtgctcgagc gcaccggtca
     4801 taccgaggcc gcagtcgacc tggcgcggct cgccggcttg aacgcgtcgg gtgtcatttg
     4861 cgagatcatg aatgacgacg gcagcatggc gcgccgtccc gagctcatcg ccttcgcgca
     4921 gcgccacggc ctcaagatcg cgacgatcgc ggatctcatt gcctatcgcc gccgccacga
     4981 ccgcatcgtc gcgcgcacag tggagacgcc cttccggagc cgcttcggcg gcgacttcaa
     5041 gatgtatatc tatgccaaca ggatcagcaa cgtcgagcat attgcgctgg tcaagggcga
     5101 catcgcgaca ccggaacccg tgttggtgcg catgcacgcg ctgaacgtgc tcgacgacgt
     5161 gctcggcaac gatttgagga accgcggcgg gctgctccat gccgccatgg aggcgatcgg
     5221 ccgcgaagga cgcggcgtgg tcgtgctcat ccgcgagacc gtttccaaca gcctctcgga
     5281 ccggctgacg agtcttctcg atcacgaggg ctacggcggc accgagctgc gcaactatgg
     5341 cgtcggcgcg cagatcctgc tcgacctcgg cgtgcgcgat atgatccttc tctcgaacac
     5401 ggagcgcacc atcgtcggcc tcgacggcta tggcctggcc gtcgtcgagc agcggccggt
     5461 cgcacggggc ggagggtcct gatggccgag gcgccacgca tcatgatcgt cgaggcgcgc
     5521 tactacgcgc atgtctccga cgagctggca cgcggcgcca ttgcggctct cgacgccgcg
     5581 ggggcgagct atgagcgctt caccgtgccc ggcgccttcg agattccggc ggcgatccgc
     5641 tatgcggtcg aggcgcagga gcgcggcgcg cgggcgacgc gcttcgacgg ctatgtcgcg
     5701 ctgggctgcg tcatccgcgg cgagaccacg cattacgatt atgtctgcgg cgaaagcgcg
     5761 cgcggcctcc aggatctggc gctggcgcat agtctcgcca tcggtttcgg catcctcacc
     5821 gtcgagaacg aggcccaggc gctggcgcgc gcccggcccg accgcaagaa caagggcggc
     5881 gaggctgcca atgcgtgcct cgccatgatc gagctgaagc gccatttcgg cctggagcgg
     5941 cgatgaccgg agacccgccc tcacgcgctc ctccggggct caggcgacgc agcgccgcgc
     6001 gcctcgccgc ggttcaggcg ctctatcaga tcgatctcgg cggcgccacg cctgaggcag
     6061 tcatcgccga gttccgcttc catcgcttcg ggcgcgagat cgacggcgag aattacggcg
     6121 aggccgacac ggccctcttc gccgagattg tgactggggt cgtcgagcgg caggccgaac
     6181 tcgaccagac gatttcgggt gcgctgacgc cggattggcc gctcgagcgg ctcgagaccg
     6241 tgctgcgcgc catcctgcgc gccggcacct tcgagctcct ggcgctcagg gaggtgccgg
     6301 cacgcgtcgt catcagcgag tatctcgaca tcgcccacgc ctttttcgcc ggcaaggagc
     6361 cgggtctcgt caatggggtg ctcgaccaca tcgcgcaccg gctgcggccc ggcgatctgg
     6421 aggagggtgg aggcggtggc ggccccgcac ctcgatgagt tcgagcgcat cgcgaagttc
     6481 tttgcgccgc tggcagcccc tggcgcgctc gatctcctcg acgatgttgc gctgatcgcc
     6541 ggtccgccgg gcgaggaata cgtgctgaag accgacgcga tcgttgaggg cgtccatttc
     6601 ttgcccgagg atccgccggc gcaggtggcg cagaagctgt tgcgcgtcaa tctgtccgac
     6661 ctcgccgcga aaggatcggc gccggtcggg tacctgctga tgaccgcctt gccgccgtca
     6721 cgcgacgagg cttggctcgc ggccttcgct caagggctgg ccgccgatca agcgacatac
     6781 ggcatcggcc tcctgggtgg cgattccgtc gcgaccgctg ggcctgtgac gctttccgtc
     6841 acggcgatcg gcaaagtcgc cgcgggacgg gcggtgctcc gccggggcgc gcgggcgggc
     6901 gatcttatct tcgtcaccgg gacgctgggc gacgccgcgc tggggctcgc cgtgctgcgc
     6961 ggcggcctcg acacgctggc gcctgcccat caggcgttcc tggtcgagcg ctatcgcttg
     7021 ccgcagccgc gtcttgtccc cgggcgccgg ctcgctggcc tcgtccatgc gatgatggac
     7081 atctccgacg ggctggtggc tgacctcggc catatctgca aggcttccgg cgtcggcgct
     7141 ctgatcgaag ccgcgcggct gccgctctcg gccgccgcgc gcgcggcggt ggccgcagag
     7201 ccggctcggc tcatggcggc acttggtggc ggcgacgact atgagctgct gttcaccgcc
     7261 gcggccgccg ccgcgggcgc gatcgccgcg ttgtcgaccg agatcggcgt cgcgctcacc
     7321 cggatcgggc ggatcgaggc cggtgccggg gtgcgcgtgc tcgacccgag cggtgcgccg
     7381 atgtccgtgc ccgtcggcgg ctatcggcac ttctgaggcc gccggttcag gggcgattag
     7441 ggattgcggt gcagagtacg gacccgaccg atttgccggc gaggagccga ttcgatcatg
     7501 gcgatggcct gctttcgccg gacgctgttg ctcgcggctt tgacgagcat ggtccttgct
     7561 gccatgcccg ctttcgccgt cgaccgcgag aaaggcctta agccgccgcc ggtcccgggc
     7621 gtaccggatt tcgtcgatct cgatccgatc gtgctgcctg tgatcgagcg gaaccgcgtc
     7681 acccgccaga tcggcctcat gctcaccctc gagctgttcg acgggcagcc cgctcaggcg
     7741 atcgacgagg agaagcgcct caggctcatg gatgccttcg tccgcgagct gcacctgatc
     7801 tatagcgcgc ggagcaccgc cgagcgcgtg gtcgacgaag acatcatcaa gcggcgcctg
     7861 ctgcgcatcg ccgatggcgt tctcggcacg ggcgtggtgc acgccatcct ggtccgccgc
     7921 ctcatcgagc aacggggcta gctcctccct ccgagggatc gtcgcgccgg tgcgatccga
     7981 ggccccgttg cctcgggccg cggcttctgg catgttccgc gcgcctttcg tcgggggacg
     8041 ataacaacaa accgaggact tgaacgatga atgcctactg gttcgtcatt gccggtggcg
     8101 tggtgctggc cctgctctac ggcttcctca cggggtctcg ggtgctggcg gccaatgccg
     8161 gcacggcgcg catgcaggag atctcagcgg ccgtgcgcga gggtgcggaa gcctatctga
     8221 accgccagta tcgcaccatc ctgatggtgg gcgtcgtcat tctcatcatc ctatgggcga
     8281 cgttgggctg gcgcagtgcc ctgggattcc tcgtcggcgc ggtgctatcg ggcgcggcgg
     8341 gctatatcgg catgaatgtg tcggtgcgcg ccaatgtgcg cacggcgcaa gcggcgcgcg
     8401 aaggcctcgc cgccggtctc agcatcgcgt tccaatcggg cgccgtcacc ggcatgctgg
     8461 tggtggggct gggcctcctc ggcgtctccg cctattactt cttcctccgg gcgacgctgc
     8521 cgggcgacga cgcgcgccgg ttcctcgaac cgctggtcgc cttgagcttc ggcgcttcgt
     8581 tgatctcgat cttcgcgcgg ctcggcggcg gcatcttcac caagggcgcg gatgtcggcg
     8641 ccgatctcgt cggcaaggtc gaggccggca tccccgagga cgatccgcgc aacccggccg
     8701 tcatcgccga caatgtcggc gacaatgtcg gcgactgcgc cggtatggcc gcggacctgt
     8761 tcgagaccta cgccgtcacg atcgtcgcca ccatgctgct ggcagccatc tacttcaccc
     8821 cgccgatgcg cgatatcatg ctgatgctgc cgctggtcat cggcggtgtc tgcatcatcg
     8881 cttcggtgat cggaacctat ttcgtgcgcc tcgggcgctc cggcaacatc atggcggcgc
     8941 tctacaaggg gcttgtcgtc tccggcgtca tctccgccgg gctcgtcgcc gccgtcatcg
     9001 ccgaactcat tggcttcgac accccgctca ccatgacgac cggcgtcacg accaccggca
     9061 ccggcatttt cggctgctgc ctcgtcgggc tcgccgtgac cggccttctg gtgtggatca
     9121 ccgagtacta cacctcgacc gcctaccggc cggtgcgcag catcgccctg gcgtcggtca
     9181 ccggacacgg caccaatgtg atccaagggc tggcggtgtc gatggaagcg acggcgctgc
     9241 cggtcatcgt catctgcatc ggcatcatcg tctcctatct gatcgccggg ctcttcggca
     9301 tcgccgttgc cgcgaccacc atgctggcgc tggccggcat gatcgtggcg ctcgatgctt
     9361 atggtcccgt caccgacaat gccggcggca tcgcggaaat ggccgacctg ccgaaggacg
     9421 tgcgcaagac gaccgacgcg ctcgacgccg tcggcaacac caccaaggcg gtgaccaagg
     9481 gctatgccat cggctcggcg ggcctcgcct cgctcgtcct cttcgccgcc tataccgagg
     9541 atctgcagca ctacttcccg aatgtctcgg tgaatttcgg cctgcagaac ccttatgtgg
     9601 tggtgggcct gctcatcggc ggtctcctgc cctatctctt cggcgcgatg ggcatgaccg
     9661 cggtgggccg cgccgcggga tcggtggtga tcgaagtgcg ccgccagttc aaggagatcc
     9721 ccggtctcat gcaaggcacg gccaaacccg attacagccg cgccgtcgac ctgctgacgc
     9781 aggcggcgat caaggaaatg atcgtgccct cgctgctgcc ggtgctggcg ccggtcgtgc
     9841 tctatctcgt gatccgtgcc atcggcggac aaggggcagc cttcagcgcc gtgggcgcca
     9901 tgctcctcgg cacgatcgtc accgggctgt tcgtcgccat ctccatgacg tcgggcggcg
     9961 gcgcctggga caatgccaag aagtacatcg aggaaggcca ccatggcggc aagggctcgg
    10021 acgcccacaa ggcggcggtg accggcgaca cggttggcga cccctacaag gacacggccg
    10081 gtcctgcggt caatcccatg atcaagatca ccaatatcgt ggcgatcctg ctgctcgcca
    10141 ttctcgcgtc gctgagcggc tcctagctca gtagccgccg cgtccaggtc cgctgggccc
    10201 gccgcccgga ttgccgccgc cgccggcgcc gccgagggga ttgttgctgc cgctgaactt
    10261 gccgaaattg ccgatgagct gctcgaggaa ggagaattcg cggccgggcg cgggcgtcgc
    10321 gttggggttg ggggtgatcg cctggcgatc ttgaagaccg cgatggcgta cgtcgcggac
    10381 gaccccgtcc ttgtcgaaat cgatcgccgt cacctcctgg tcgatgagct ggggcttgaa
    10441 gaaggcggcg tccttggtct tctggctgac gtaataccac gtgttcggct cgaaggcggc
    10501 gacgctcgag ggcgagccca ggaggcgggt caccgtcgcc ttgtcggttg cgcccggcgt
    10561 gatctggtcg agcgcgtcct tcgcgggcaa gttgccgcgc tggtcgacgg ggttggagca
    10621 ggcggcaacc gccagcacgg cgatgccgga agcaagcgcg cgaattggag gaaggcgtcg
    10681 gatcatgacg gggactcgaa ggtgagcggg cgttcgaagg tagcccggat cggccgccat
    10741 tccaagcatg ctggcatgcc ggcggcgatt gacagggcgg gacgccgcac ccatctggcg
    10801 tcacggcgag gagcgatggg cggcctgctc aaatcatggc atcggcgccg cgagcgccgg
    10861 tcggcaatgc tcggcgccta tcggcagatc gtcgatcggg cgcgcgagcc tgtgctgttt
    10921 cacgtctggg gcgtgccgga tacactggat gggcgattcg aagtgctggc gctccacgcc
    10981 tttctcgtgc ttcatcgcct gaagcgcgag ggggaggcga cggcggaatt cgcgcaggcg
    11041 ctcttcgata cgatgttcgc cgatctcgac ggggcgttgc gcgagatggg agcgagcgat
    11101 ctcggcgtcg ggccgcgtat caaagccatg gcgcggggct tttacggtcg cgtcgccgct
    11161 tacgagcgcg gcttgggcga ttcgggcgac ctcgaagcgg cgctccggcg caatctcttc
    11221 ggcacgattc ggccggggga ggcgcaggtg gaagcggctt cacgctacct gcggcagcag
    11281 gtagcggcgc tcgcggcggc gccggtcgag gcgctgctcg agggcagagt ggcctttgcg
    11341 gctctcgccg gagcgggtga ataaggttaa tcctacggta atcttcgtcg tttatagcca
    11401 tagaagttgc cagaattcag gtttttgggt acttagcagc ggcttttgcc cctgaatggg
    11461 ggctctttca gagtggttcc atggcagtcc caaagaagaa ggtctccaaa tcgcgtcgca
    11521 acatgcgccg cgcgcatcac gccctcgaag cagtgagcta tgccgaatgc tcgaactgcg
    11581 gcgagctcaa acggccgcat cacgtgtgcg cggcctgcgg ccattacgac ggccgcgaag
    11641 tggtggccgt cccggcggag acggcgtgag cgaccctgcg gggatcggac gggcttgagc
    11701 gaccgcgtca ccatcgcgct cgatgccatg ggcggcgacc gcgcccccga catggtgctg
    11761 aagggcgcaa gcatcgcgct gcagcgcttc ccgcaagttc atttccggat ctacggcatc
    11821 gagagccaca ttcgcccgct catggcgaag ctgccgcggc tcgccggcaa ggccacgctg
    11881 catcacaccg atgatgtcgt gaccgacgac gccaagccgt cgacggcgct gcgcaccggc
    11941 cggcgctcga gcatgcgcct cgccatcgac gcggtggcgg agggtgaggc cgacggcgtc
    12001 gtctccgccg gcaataccgg cgcgctgatg gcgatggcga agttcgcgct caagatgctg
    12061 ccgggaatcg atcgtccggc catggcgtcg ttctttccga cgcggcgtgg cgagagcgtc
    12121 atgctcgacc tcggcgccaa tgtcgaatgc gatgccgaca atctcgtgca attcgccgtc
    12181 atgggcgacg tcttcgcgcg caccgtgctg ggcctgctgc agccgacggt cgggctcctc
    12241 aatgtcggct ccgaggacct caagggtaat gacgccgttc gcggcgccag cgcgcagctg
    12301 cgcggcgccg gcttgccgat ccgcttctac ggcttcatcg agggcgacga catcggcgcc
    12361 ggcaccgttg acgtcgtcgt caccgacggt ttcaccggca acgtcgcgct caaggtgatg
    12421 gagggcacgg cgcgacttta tggcgagttc ctgcgcgcct ccttcaagaa ttcgctgacc
    12481 gcacgcatcg gctacttgct cgctcgccgc tccctgcaca agctgcgcga tcgcctcgac
    12541 ccgcggcgcc acaacggcgc cgtgttcctc ggtctccagg gcatcgccgt caagagccac
    12601 gggagcgccg atgcgttgag cttcgccaac gccataggcg ttgccgtcga catgaaggtc
    12661 aacgggttcc tcgagcagat ccgcgacggc gtggcgcggc tgcatgcgct gcaggcgccc
    12721 gtggagaagc cgcaatcggc cgctctctga tatgatgttt cgcgcgcagg tcatcggttg
    12781 cggcgcctat ctgccggagc gcgtcgtcac gaacgccgag ctggcgcagc gcctcgacac
    12841 ctccgacgag tggatcgtcc agcgcaccgg catccgccag cggcatatcg cggcgccggg
    12901 cgagctcacc tcggatttgg cgttccgcgc gtctcgcgat gcgctggccg cggcgggcct
    12961 caaagggagc gacctcgatc tcatcgtgct ggcgacggcg acgcccgaca acaccttccc
    13021 ggcgacggcg accaagctcc aggcgcggct tggcatgacc aaaggcgccg ccttcgatgt
    13081 gcaggcggtg tgctcgggct tcgtctacgc gctcgcggtc gccgacaatt tcatccgcag
    13141 cggccaggcg cagacggcgc tggtggtcgg cgccgagact ttctcgcgca tcctcgattg
    13201 ggaggaccgc ggcacctgcg ttctgttcgg cgacggcgcc ggcgcggtcg tgctgcgcag
    13261 cgtggcgtcg cgcggcgctg cgaccgagcg cggcgtgctc tcgacccatc tccattccga
    13321 cggcaccggc tacgacctcc tctatgtcga tggcgggccc tcgaccacgg ggagcagcgg
    13381 cttcttgcgc atggaaggca aggaaatctt ccgccaggcg gtgcagcggc tggccgaggt
    13441 ggtggacgag gcgcttgccg ccaacaagct caccgcccgc gacgtcgact ggctcgttcc
    13501 ccatcaggcc aatcgccgca tcatcgacag catggcgcgg aagctcggcc tttccggcga
    13561 caaggtggtg atgacgatcg agcgccacgc caatacctcg gccgcctcgg tgccgctggc
    13621 gctcgccgag gccgtcgccg acggccgcat caagcccggc catctcgtgc tcctcgaagc
    13681 catgggcggc ggcctcacct gggccgccgg tctcctgcgc tggtgatgcg ccgcggcaag
    13741 gcccgcgtgt agcgcgactc gacggcaatt gattcatctc ccgcagcggt ctaacgttcc
    13801 caaaagggga ggccaaggac tatggcgggg caaacggtga cgcgcgctca attgagcgag
    13861 gcggtctatc aggaggtcgg cctgtcacgc aacgagtcgg cggagctggt cgagacggtg
    13921 ctgaacgaga tctcgaccgc gctcgcgaag ggcgagatgg tcaagatctc ctccttcggc
    13981 agctttgcgg tccgccgcaa gggcaagcgc atcgggcgca accccaagac gggcaaggag
    14041 gtgccgatca acccgcggcg cgtcctcgtg ttccgcgcct cccatgtttt gaaggatcag
    14101 atcaacgcgt cgctcgccaa ggtcggtctc gcccgcagcg gagccgcctg accatgcccg
    14161 tcgatctcat gagcccgccc gcggccgacg ctgcggagcg cgcgtcgcgc cgccagcaga
    14221 aatccgctga cgccttccgc acgatcagcg aggtggctca ggagctggag attccccagc
    14281 acgtgctacg cttctgggag tcgcgctttg cccagctccg tccgctgaag cgcgcgggcg
    14341 gccgccgcta ctatcgtccc gatgacgtcg tgctgctgcg gcgtatccgc caatgcctct
    14401 acgatcaggg ctacacgatc aagggcgtgc agaagctgct gcgcgaaggc gccctcaaaa
    14461 gcgaagccgt gctgcgcccc acggcagcgg ccccggtcgc cgtgacgccg gcagcgccgc
    14521 gcgtcaaaga cggcggcaat cgcacccatc tcgtcgagct ccggcgcgag ctcgtcgagt
    14581 tgcgcgatct cttgcgccgc gcccggcgct gagcgttgcg ccgatccggg gccaacacta
    14641 tagtcggccc gtcgggcatt agcgcagcct ggtagcgcac cagcatgggg tgctgggggt
    14701 cgtcagttcg aatctgacat gcccgaccat tttctcccgc gaagacggaa tggcaggatc
    14761 cgacgcgatt ctccgattac cgggccgtcg atctgacgag cctgcggaac gatggggtcg
    14821 atctcttcgc ctcgcccgag tccgctaatc caacccggct cggcggaagg cttctatata
    14881 gtgctcgcgt tcggactcct gcttgatcgg catctgcttc gcgatccagg caagcgagat
    14941 gttaggctgc gcgcggcgaa gctcctggag agccgctgcg gcgacctcgg ccgcgccggc
    15001 catggcggcc gaagcggtga gcacgcggtg cgcgccgacg aaatcggccc gctgccggag
    15061 cccctggcgc gccagccgca tcgcctcctc gtagttgcgt ccgatgaact gagcgtaggc
    15121 ggcgatgccg taatagacgc ccgagaacgg atcgcggggg ctcagccgca gcgcacggtt
    15181 cgcggccacc aagccttcct gccagcggcc gcaataacac agcgtcaagc cgtaatagcc
    15241 ttgggccatg gagaagttcg gattgagccg tagcgccaat tcgaattcgg cgagcgcgtc
    15301 gtcgaagcgc cgcgcaaata aacaagcggt ggccagggcg ttgtgcgccc aggcgtcctc
    15361 gctgtccacc cggatcgccg caagcgccgc ccgctccgcg atcggcatga cggtcgccat
    15421 gtccgcccag cccatatggg cgctgaagct gtggctggca gcgagcacgc cgagcgcctg
    15481 accatagttc ggatcgatcg cggtcgcctt ctcgagcaga gcctgcgcca ccacgttgtc
    15541 ctgccgtgtg acgcgccaga aatgcgagag cgcccgcatc accagatccc aggcgtccag
    15601 gctgtccggc ggcttgcgct gggcgcgaaa attttccgcg gcgtagagcc gcggctcgat
    15661 cgccgcgacg atcgactcgg tgatctcgtc ctgcacggcg aagacgtcgg cgaggccgcg
    15721 atcgtagcgc tcggcccaga tatggccgcc gctggcaacg tcgttgacct gcgccgtgat
    15781 gcgcacgaga tcgccgctct tgcgcacgct gccctcgacc acatagccga cgccgagctc
    15841 cgcggcgatc tgcttcatgt gcaccggttt gcccttgtag atgaaggacg agttgcgcgc
    15901 gatgacgaag aaccagcgca gcttcgagag cgccgtgatg atgtcctcgc tgatgccgtc
    15961 ggaaaaatac tcctgctcgg gatcgccgct catgttcgtg aagggcagca cggcgatcgc
    16021 cggccggtcg ggaagcggca gggccgagcg cggcgcctcg tgaaattcac gagacgccgc
    16081 tgtcacggcg tcggccgttt cctcgctcgg cgattgggga tgcaccgtgc ccacgaagcg
    16141 caggcccttg cgcgcaatcg tgcggatcag cctttgcgcg tcgccgctgt cgccgaccgc
    16201 ctttcgcgcg gcgttgacgc ggctggtcag ggtcgagtcc gagacgatcc ggccgcccca
    16261 gaccgaagcg atgagatcat ccttgctgac gacgtggtcg cgattctcca gcagataggt
    16321 caggagatcg aagacctgcg gctcgacggc gatcgcctcc gaaccgcggc agagctcgcg
    16381 ccggtcggta tcgagcgtat ggccagcaaa gacaaaccgc atgtcggggc gctcagcgga
    16441 tggcacgggt c
//
LOCUS       DASYNS010000124         5299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4397288, whole genome shotgun sequence.
ACCESSION   DASYNS010000124 DASYNS010000000
VERSION     DASYNS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4397288"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..696)
                     /locus_tag="VGP50_03975"
     CDS             complement(187..696)
                     /locus_tag="VGP50_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEV8026559.1"
                     /translation="MTTMAKPGRNDFCPCGSGNKYKKCCLPKEEAAERKQLAKAEARR
                     LESAAAHRLPLREVKAAIAAILSDAGDAEEDELTIASNAAADLVRAGKLDEAEQAARD
                     LQVRFPEVHDGYDRLGMVHEARGENRQAADCYRKVIAFVREHPDDYDPGFEDVFVKLV
                     NRLDPPGAT"
     gene            725..1294
                     /locus_tag="VGP50_03980"
     CDS             725..1294
                     /locus_tag="VGP50_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026560.1"
                     /translation="MYSTLLADATFHELLLVFDQDIAIATRRGRCPLCSSVLHSAQYR
                     RKPRGRPAGLGEEHDWRFSFCCARDGCRTRRTPPSLRFLGRKVYLAAIVVLIAIMREG
                     ATAARMRQLSELIGVNRRTVERWREWWRDTFTASPFWQIGRAAFMPPVDHDRLPVSLI
                     ERFNGGGDERLIALLRFLAPITGGDRRGR"
     gene            1385..2848
                     /locus_tag="VGP50_03985"
     CDS             1385..2848
                     /locus_tag="VGP50_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEV8026561.1"
                     /translation="MTQQARGPRVHERWAHLRFSVIGQLLAAPPPKGELRAQIEALAG
                     RQWRRPITGEPVRFGFSTIERWFYRALKERSDPVGVLRRKLRTDAGQQPAMCDAVRQV
                     VLAQYAAHKSWSVQLHHDNLVALAQTRDDLQPLPCYDTLRRFMKVNGLDKRRRVTSRQ
                     TAGADRAEARLFDREVRSYEAAYVNGLWHWDCHHGSRKVLTARGEWRTPILFGVLDDR
                     SRLACHLQWYLAETAEIIAHGLSQAFQKRGLPRSGLSDNGAAMTAAEITEGLNRLGIL
                     HQTTLPYSPYQNAKQEAFWGPVEGRLIAMLEDVPDLTLAVLNEATQAWVEYEYNRKVH
                     SEIGQAPITRFLAGPEVTRPSPDSDTLRLAFTRTDHRMQRKSDGTVVIEGRRFEIPNR
                     YRHLSRIEVRYAGWDLGLVHLVDERTGKALSRLYPQDKTENASGLRRSLDPISPEPID
                     VKPATGIAPLLARLIDRQTATGLPPPYLPKDERGDDT"
     gene            2845..3690
                     /locus_tag="VGP50_03990"
     CDS             2845..3690
                     /locus_tag="VGP50_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8026562.1"
                     /translation="MNKKLLALYGLKWNPFAPDVPVEALHATPRIESFCWRVQQLVGE
                     GGFALVTGAPGTGKSVTLRILAERLSQQRDVKIGLCSRPQANLADFYREMGDLFGVEL
                     RPHNRWAGAKVLRERWQTHIDASLARPVLVVDEAQEMLSTVLSELRLLCSTRLDSHIL
                     LTVVLAGDGRLLERLRSDEFLPLASRMRVRLAIERATPDELQECLRQALHKAGAVKLM
                     TPELIATLCDHAQGNLRALMNMAGELLVYAAEREARQIDEKLFLETCAAPPAADAKAA
                     AGRRR"
     gene            3687..4646
                     /locus_tag="VGP50_03995"
     CDS             3687..4646
                     /locus_tag="VGP50_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8026563.1"
                     /translation="MSLLPVEPAWRLADRAEDQRWLVTGLWSEQAVGIIGGEPKCCKS
                     FLALDLAVAVAAGVPCLRRFAVPRAGRVLLYAAEDALHIVRRRLDGICAAAGLGLADL
                     DVQVITAPAVRLDLEADRRSLDVTVAEWRPRLLVLDPFVRLHRIDENASGEVAPLLAY
                     LRELQRRHAIAVIVVHHAKKGAGHVRAGQALRGSSEFHAWGDSNLYLRRNRDLRESGD
                     ITLTVEHRAAPAMPSVTLELAQRANALALEVIEPRTPQTPTPSSIDERITAALTDVNR
                     PLPFAELRARCRVRTATLYERLAALTAAGRLGKSDQGYRLAGR"
BASE COUNT          873 a         1877 c         1671 g          878 t
ORIGIN      
        1 ccgttgattt cgctcccccc caactggccc caagctctgc gattttcatc gttcaggccg
       61 cgccgcccgc tcagagcaac ataacactgc ggcgggaagt tgaggtgggc ccgggaggac
      121 ttgaggatag gtttgtgacg gtttctggtt ttggaccctc acggagcgcg agcggagtgg
      181 ccacgctcag gtggcgcctg gcgggtcgag ccggttgacg agtttaacga acacgtcctc
      241 gaagccgggg tcgtagtcgt cggggtgctc cctgacgaac gcgatcacct ttcgatagca
      301 gtcggcggcc tgtcggttct cgccacgggc ctcgtgcacc atgccgaggc ggtcgtagcc
      361 gtcgtggact tcggggaacc gcacctgaag gtctcgggcg gcctgctcgg cctcatcgag
      421 cttgccggca cggacgaggt cggcagcggc gttggacgca atggtcagtt cgtcctcttc
      481 ggcgtcgccg gcatcggaga gaatcgccgc gatggcggct ttcacctcac gaagcggcaa
      541 acgatgagcg gcggcgcttt cgaggcggcg agcttcggcc ttggcaagct gcttgcgctc
      601 agccgcttcc tccttgggaa ggcaacactt cttgtacttg ttgccactgc cgcaagggca
      661 gaaatcattg cgtccaggtt tggccatcgt ggtcaatgac cttgagctga gaagggcgca
      721 tccgttgtat tcaaccctgc tggccgacgc cacattccat gagctgcttc tcgtctttga
      781 tcaggacatc gcgattgcga cgcggcgggg acgatgcccg ctgtgcagca gcgttctgca
      841 ttctgcccag tatcgccgca agcctcgtgg ccgtccggcc gggctcggcg aggagcatga
      901 ttggcgcttc agcttctgct gcgcccgcga cggttgccgc acacgcagaa cgccgccgtc
      961 gctccggttt ctcgggcgca aggtctatct cgccgcgata gtagtgctga tcgcgatcat
     1021 gcgggaaggc gcgaccgccg cgcggatgcg acagctgtcc gagttgatcg gcgtcaaccg
     1081 ccggacggtc gagcgctggc gagagtggtg gcgcgacacc ttcacggcga gcccgttctg
     1141 gcagatcggc cgggccgcat tcatgccgcc cgtcgatcac gatcgcctgc cggtctcgct
     1201 gatcgagcgc tttaacggcg gcggcgacga gcggctcatt gcgctgttgc gcttcctcgc
     1261 cccgatcacc ggaggcgaca ggcgcggtcg ctaacggctt ttcccgatcc gcagaggatg
     1321 ctcatcgccg gcagtccagg cctccatcta cggtccgtgc gccctgatcc agaggagcgc
     1381 cggcatgaca cagcaggcac gaggtccccg agtccatgag cggtgggcgc atctgcgctt
     1441 ctccgtaatt ggacagctgt tggcggcgcc gccgcccaaa ggcgagttgc gcgcccagat
     1501 cgaagcgctt gccgggcgcc agtggcggcg ccccatcacg ggcgaacctg tccgcttcgg
     1561 cttctccacc atcgagcgct ggttctaccg cgccttaaag gagcgtagcg acccggtcgg
     1621 cgtcttgcgc cgcaagctgc gcaccgatgc cggccagcag cccgcgatgt gcgacgcggt
     1681 gcgccaggtc gtgctcgcgc agtatgccgc gcacaagagc tggagcgtcc agcttcatca
     1741 cgacaatctc gtcgcgctcg cccagacgag agacgatctg cagcctttgc cctgctacga
     1801 cacactgcgc cgcttcatga aggtgaacgg tctcgacaag cggcgtcggg tgacatcacg
     1861 ccagaccgcc ggcgccgacc gcgccgaagc gcgactgttc gaccgcgagg tgcgcagcta
     1921 cgaggctgcg tacgtgaatg gactatggca ctgggattgt caccacggct caaggaaggt
     1981 gctcaccgca cgcggcgagt ggcggacacc gatcctgttc ggcgtgctgg atgatcgctc
     2041 ccggcttgcc tgtcatctgc agtggtatct ggccgagacg gccgagatca tcgcgcatgg
     2101 cctgtcgcag gcgtttcaga agcgcggcct gccgcgttcc ggcctgagcg acaatggcgc
     2161 agccatgacg gccgccgaga tcaccgaggg gctcaaccgc ctcggcatcc tgcaccagac
     2221 cacgttgccg tacagccctt accagaatgc caagcaagag gcgttctggg gcccagtcga
     2281 aggccggctc atcgccatgc tggaggatgt gcccgatctc acgcttgcgg tcctcaacga
     2341 agcgacgcag gcctgggtcg agtacgagta caaccgcaag gtccactccg agatcggcca
     2401 agcccccatc acgcgcttcc tcgcgggtcc ggaggtgacg cggccgagcc ccgacagcga
     2461 cacgctgcgc ctcgccttca cccgcaccga tcaccgcatg cagcgcaaga gcgacggcac
     2521 tgtcgtcatc gaggggcggc gcttcgagat tccaaaccgc taccgccatc tctcccgcat
     2581 cgaggtccgc tacgccggct gggatctcgg cctcgtccac ctcgtcgacg aacgcactgg
     2641 caaggcgctg tcccggctct atccccagga caagaccgag aacgccagcg gcctgcgccg
     2701 ttcgctcgat ccgatctcgc ccgagcccat cgacgtcaag ccggcgaccg gcatcgcgcc
     2761 gctgctcgcc cggctgatcg accggcagac agccaccgga ctgccgccgc cctatctccc
     2821 caaggacgag cgaggagacg acacgtgaac aagaagctgc tcgcgctcta tggcctcaaa
     2881 tggaacccgt ttgcgcccga cgtgccggtc gaggcgctcc atgccacgcc gcgcatcgag
     2941 tcgttctgct ggcgcgtcca gcagctggtt ggcgaaggcg gcttcgccct tgtcaccggg
     3001 gctcccggca caggcaagtc ggtcaccttg cgcattctcg ccgagcggct ctcgcagcag
     3061 cgtgacgtca agatcggcct gtgcagccgg ccccaggcca atctcgccga cttctatcgc
     3121 gagatgggcg acctgttcgg cgtcgagctc cgcccccaca accgctgggc cggcgccaag
     3181 gtgctgcgcg agcgttggca gacccacatc gatgcctcgc tggcgcggcc ggtcctggtc
     3241 gtcgacgagg cgcaggagat gttgtcgacc gttctctccg agttgcgcct gttgtgttcc
     3301 accaggctcg actcacacat cctgctcacg gtcgtactcg ccggcgacgg tcggctcctc
     3361 gaacgcttgc gatccgatga gttcctcccc ctcgccagcc gcatgcgggt gcgcctggcg
     3421 atcgagcgcg ccaccccgga cgaactccag gaatgtctca ggcaggcgct gcacaaggcc
     3481 ggcgccgtaa agctgatgac tccggagctg atcgcgacgc tttgcgatca cgcccagggc
     3541 aacctgcgcg ccctcatgaa catggccggc gagctgctcg tatacgccgc cgagcgcgag
     3601 gcccggcaga tcgacgagaa gctgttcctg gagacgtgcg ccgccccgcc agctgccgat
     3661 gccaaggccg cagcagggcg acggcgatga gcctgttgcc agtcgagccc gcctggcgcc
     3721 tcgccgatcg cgccgaggac cagcgctggc tcgtcactgg gctgtggtca gagcaggcgg
     3781 tcgggatcat cggcggcgag cccaagtgct gcaaatcttt cctcgccctg gacctcgccg
     3841 tcgcggtcgc ggccggcgtt ccgtgcctgc gccgcttcgc cgtgccacgc gctggacgcg
     3901 ttctgcttta cgccgccgag gacgcgctcc acatcgtgcg ccggcggctc gacggcatct
     3961 gcgccgccgc cggcctcggc ctcgccgatc tcgacgtcca ggtcatcacc gcacccgccg
     4021 tgcggctcga tctcgaggcc gaccgcagaa gcctcgatgt gaccgttgcc gaatggcgcc
     4081 cgcgcctgct cgtcctcgat cccttcgtgc gcctgcatcg catcgatgag aacgccagcg
     4141 gcgaagtggc gccgctgctc gcctatctgc gcgagttgca gcgccgccac gccatcgccg
     4201 tcatcgtcgt ccaccacgcc aagaagggcg ccggccacgt ccgcgccggt caggccttgc
     4261 gaggctcctc cgagttccat gcctggggcg attccaacct ctacctccgc cgcaaccgcg
     4321 atctccgcga aagcggcgat atcaccctga ccgtcgagca ccgcgccgct ccggccatgc
     4381 ccagcgtgac actcgaactc gctcagcgcg ccaacgctct cgcgctcgag gtcatcgaac
     4441 cgcgaacccc gcagacgccg actcccagtt ccatcgacga gcgcatcact gccgcactca
     4501 ccgatgtcaa ccgcccgctg ccgttcgccg agctgcgcgc gcggtgccgc gtccgcaccg
     4561 ccaccctcta cgagcgcctc gccgccctca ccgccgccgg ccgcctcggc aagtccgacc
     4621 aaggttatcg cctcgccggt cgctgacaga gcacgcgcga caatcagccg ggaacacagc
     4681 cacaatcggc gggcacgaca tcgcgccggc tgccgcttcc gcttccggtt ctctctacac
     4741 cgtcgtggaa gcagcagcgg gaagtccatc aagatgtgtc tccgcagccc cgcgtgcccg
     4801 gcgcgccatc cttctgttcg atcgccgcca tccttccgtc ccgacctgcg cgcgcgacat
     4861 gccggcgcgc gcaacggcgg tcaaggatgg cccgtcttcg ggccaccgcg ccagcggcgc
     4921 gaagcgtcct tgacggccgc gagcacgacg gcaggctcca ccgcgtcggg gctgcgatct
     4981 ccctatccaa caaacaaacc atcaaaaagc ggccgccaaa gaccatcaaa aaatgaactg
     5041 acgcttaaag gcagttcaac gcgccggacc acgtcggtac gacaagaaga tcgagctgtg
     5101 gttttccaac gaccggttcg aacggatcgc aggcggaaag cgcgagggcc aggctctgaa
     5161 gaagatgctg gccaaaatgg gtcgcttgat gatttggggg cgcgggaagg cgccgcccgg
     5221 cagcgtggac agccgaagct gaaggaggct tgaccgccat cgtccgaacc cagcggaagg
     5281 cgtcatgtta ctgacttcc
//
LOCUS       DASYNS010000125         4421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3149096, whole genome shotgun sequence.
ACCESSION   DASYNS010000125 DASYNS010000000
VERSION     DASYNS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3149096"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /gene="folE"
                     /locus_tag="VGP50_04000"
     CDS             complement(<1..562)
                     /gene="folE"
                     /locus_tag="VGP50_04000"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV8026564.1"
                     /translation="MTANVHALEPIRAPHTERRRVERSEAEAAVRTLIRWAGDDPGRE
                     GLLETPARVTRAIEEFYAGYAEEPAAILQKSFEEIEGYDEMVVLRDIRFESHCEHHMA
                     PIIGHACVAYVPRGRVVGISKLARVIEVHARRLQIQEKLTAQIANTIDQVLKPHGVGV
                     VIKALHHCMTTRGVHKPDTSLVTSRML"
     gene            complement(668..1786)
                     /gene="ald"
                     /locus_tag="VGP50_04005"
     CDS             complement(668..1786)
                     /gene="ald"
                     /locus_tag="VGP50_04005"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV8026565.1"
                     /translation="MLVGVPKEIKDNEYRVGLVPSTVRELTDKGHQALVEAGAGSGAG
                     LSDGDYKSAGAEIVADADAVYGRAELIVKVKEPLAAERRKLRHGQVLFAYLHLAPDRE
                     QTEDLLKAGVTAIAYETVTGRDGALPLLTPMSEVAGRMAPHVGARCLEKENGGRGVLL
                     AGVPGVSPASVVILGGGVAGTNAALIACGMGADVAVLDRNPDALRRVAVQLGNRARTL
                     FSTRDVVELLCRRADLVIATVLVPGATAPKLISAQTVKAMKPGAVIVDVAIDQGGCAE
                     TSRPTTHSHPTYVVDDVVHYCVTNMPGAVARTSTFALSNVTTSFVLALADKGWRRALS
                     EDEHLRDGLNVHVGRVTYRAVAEALRLPYTHAEQALRI"
     gene            complement(1804..2334)
                     /locus_tag="VGP50_04010"
     CDS             complement(1804..2334)
                     /locus_tag="VGP50_04010"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEV8026566.1"
                     /translation="MDWIVGWLGRHNIELSVVLATFVLVAVASVLIFLVNRLLKKWLQ
                     PGEARLGLRTEDIATLVRVVTAILWFVTLLVAFDLWGIGFGGFWTVLVSAATIIGAAF
                     VATWAMVSNVTADLFLTIWRPFRLGDTVELLPEKLKGRAIDRNLMFTALLEEDGCVLQ
                     VPNNLFFQRIFRVGRS"
     gene            complement(2375..2983)
                     /locus_tag="VGP50_04015"
     CDS             complement(2375..2983)
                     /locus_tag="VGP50_04015"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEV8026567.1"
                     /translation="MELTRQDYEAVSAAIHDAETRTSGQIVCVLAHASSDYAYVPPLW
                     ASALALVAPWPLIMFTPWSVQRIFSIQLAVFIVAALIFSLPRLRLALVPRAVRRARAH
                     RAALEQFVVRRIAHTRNRTGVLIFVSLAERYARIIADEAIAQKVPNAEWQAAVDALTA
                     HMRDGRIAQGFTAAIERCGAVLAAHAPADGAPNELPDRLYVM"
     gene            complement(2983..3759)
                     /locus_tag="VGP50_04020"
     CDS             complement(2983..3759)
                     /locus_tag="VGP50_04020"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEV8026568.1"
                     /translation="MAAGLVLCLALSALAFDFPPLTGRIVDQAGVIKSGTRNDLEEML
                     KGLEDKSGIQLVVATVKSLQGSDIETYANQLFRFWKLGEAKKNNGVLLLVAPAEHKVR
                     IEVGYGLEGTLTDALSSVIVSSAIIPRFKTGDFSGGIERGVEGIISVLSGDTADWQPK
                     PAVRHDDPAGVLNALFPFLFFLAIILIFRFLVGNVSNTTAGRYVNRGGRTVFVPYSGS
                     GWGSSWGGGSSSGGGFGGDGGGGFSGGGGSSGGGGASGSW"
     gene            complement(3843..4415)
                     /locus_tag="VGP50_04025"
     CDS             complement(3843..4415)
                     /locus_tag="VGP50_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004732432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV8026569.1"
                     /translation="MFLAVIGLGLTLSGCGYNTIPQLQETAKARWSDVQNQYQRRADL
                     IPNLVETVKGYAAQEKNVLTAVVEARAKATQTKIDVSQLTDPDKLKAFQDAQNQLTGA
                     LGRLLAVSENYPDLKSNANFLALQSQLEGTENRIAVARRDYIDAVRAYNTALRTFPTL
                     LWAKFFFSGNKPMAEFTATEGSDKPPPVKF"
BASE COUNT          780 a         1456 c         1482 g          703 t
ORIGIN      
        1 ctagcatacg gctggtgacg agactcgtgt ccggcttgtg cacgccgcgc gtcgtcatgc
       61 agtggtggag cgccttgatg acgacgccga cgccgtgcgg cttgagcacc tggtcgatcg
      121 tattggcgat ctgcgcggtg agcttttcct ggatctgcaa gcgcctggca tggacctcga
      181 tcacgcgcgc gagcttgctg atgccgacca cgcggccgcg cggaacatag gcgacgcagg
      241 cgtggccgat gatcggtgcc atgtggtgct cgcaatggct ctcgaagcga atatcgcgca
      301 acaccaccat ctcgtcatag ccctcgatct cctcgaagga tttctgcagg atggcggcag
      361 gctcctcggc atagccggca tagaactcct cgattgcccg ggtcacgcgc gccggcgtct
      421 ccaggaggcc ctcgcggccg gggtcgtcgc cggcccagcg gatgagggtc cgcactgcgg
      481 cctcggcctc cgacctctcc acccggcgcc gttcggtatg gggtgcccgg atcggctcca
      541 gcgcgtgcac attcgcggtc atgatcatgc tctcctcgac gggcaggacc cggacccagc
      601 ccggctttgc cagcctcgac ggagcattag atggacgaga gacggaaagg ttccgccgat
      661 acggcgctca aatgcgcagc gcctgctctg cgtgcgtgta agggagccgg agcgcctcgg
      721 cgacggcgcg gtaggtgacg cgcccgacat gcacgttgag cccgtcgcgc agatgctcgt
      781 cttcgctcag cgcccgccgc cagcccttgt cggcgagcgc gagcacgaac gaggtggtca
      841 cattgctcaa ggcgaaggtc gaggtgcgcg ccacagcgcc gggcatgttg gtgacgcaat
      901 agtgcaccac gtcgtcgacg acataggtcg ggtgcgaatg cgtggtcggc cgcgaagtct
      961 cggcgcagcc gccctggtcg atggcgacgt cgacgatcac cgcgccgggc ttcatcgcct
     1021 tcacggtctg cgccgagatc agcttcggcg cggtcgcacc cggcaccagg accgtggcga
     1081 tgacgagatc ggcgcggcgg cagagcaact cgaccacgtc gcgggtggag aacagcgtgc
     1141 gcgcacggtt gccgagctgc acggcgacgc ggcgcagcgc gtcgggattg cggtcgagga
     1201 ccgcgacgtc cgcgcccatg ccgcaggcga tcaaggcggc attcgttccg gcaacgccgc
     1261 cgccgaggat gacgacgctc gccggcgaaa cgccgggtac gccggcgagc agcaccccgc
     1321 gcccgccatt ctccttctcc aggcagcgcg caccgacatg cggcgccatg cggccggcga
     1381 cctccgacat cggcgtgagc aaagggagcg cgccgtcgcg gccggtcacc gtctcgtagg
     1441 caatggcggt gacgccggcc ttgagcaaat cctcggtctg ctcgcggtcg ggcgcgagat
     1501 gcagataggc gaacagcacc tggccgtggc ggagcttcct gcgctcggcg gcgagcggct
     1561 ccttcacctt cacgatcagc tcggcgcggc catagacggc atcggcgtcg gccacgatct
     1621 cggcgccggc ggatttatag tcgccgtcgg agagcccggc ccccgatccc gcgccggcct
     1681 cgaccagcgc ctggtggccc ttgtcggtga gctcgcgcac ggtcgaaggc accaggccga
     1741 cgcggtactc gttgtccttg atctccttcg gtacgccgac cagcatggca atgtcctcga
     1801 cgctcagctg cggccgacgc gaaaaatccg ctggaagaag agattattgg gcacctgcag
     1861 gacgcagcca tcctcctcga gcagcgccgt gaacatcagg ttgcggtcga tggcgcggcc
     1921 tttgagcttt tccggcagca gctcgacggt atcgccgagg cgaaacggcc gccagatggt
     1981 gaggaagagg tcggcggtga cgttgctgac catcgcccag gtggccacga aggcggcgcc
     2041 gatgatcgtc gcggcgctga ccagcaccgt ccagaaaccg ccgaagccga tgccccatag
     2101 gtcgaacgcc accagcaagg tgacgaacca gaggatggcg gtgacgacgc gcaccagggt
     2161 ggcgatgtcc tcggtccgca gcccgagccg cgcctcgccg ggctgcagcc atttcttcaa
     2221 caggcgattg accaggaaaa tcaggacgga cgccacggcg acgagcacga aggtcgccag
     2281 cacgaccgac agctcgatat tgtgacgccc aagccatccg acgatccaat ccatgaccgc
     2341 atttcccgat taggcgggcg ccgcccgctc gctattacat cacataaagc cggtccggca
     2401 attcgttggg tgcgccgtcg gccggagcat gcgccgcgag cacggcgccg caacgctcga
     2461 tcgccgcggt aaagccttgg gcgatgcggc cgtcgcgcat gtgggcggtc agcgcgtcaa
     2521 ccgccgcttg ccattccgca ttcggcactt tctgcgctat cgcctcgtcg gcgatgatgc
     2581 gcgcgtaacg ctcggcgagc gacacgaaga tgagcacgcc ggtgcggttc ctggtgtggg
     2641 cgatgcggcg gacgacgaac tgctcgagcg cggcacggtg tgcccgcgcc cggcggaccg
     2701 cgcgcggcac cagcgccagc cgcagccgcg gcaacgagaa gatcagggca gcgacgatga
     2761 agaccgcaag ctggatcgag aaaatccgct gcacgctcca tggcgtgaac atgatgagcg
     2821 gccatggcgc gacgagcgca agcgcgctcg cccagagcgg cggcacatag gcgtagtccg
     2881 aggaggcgtg ggcgagcaca cagacgatct gtccgctggt ccgcgtctct gcgtcatgga
     2941 tcgcagccga gaccgcctcg taatcctgcc gggtcagctc catcaccagc tccccgatgc
     3001 gccgccgccg cccgacgacc cgccgccgcc ggagaagccg ccgccgccgt cgccaccgaa
     3061 accgccgccc gaagacgatc ctccgcccca gctcgagccc caaccggagc cgctgtaagg
     3121 aacgaacacc gtgcggccgc cgcgattgac gtagcgcccg gccgttgtgt tgctcacatt
     3181 gccgaccaga aatcggaaaa tgaggatgat cgccaggaaa aacaaaaacg gaaagagcgc
     3241 attgagaaca ccggcgggat cgtcgtgacg tacggcgggc ttcggctgcc aatcggcagt
     3301 gtcgccgctg agcacgctga taatgccttc gacgccgcgc tcgatgccgc cggagaaatc
     3361 gccggtcttg aaccgcggga tgatggcgct cgagacgatg accgaggaaa gcgcgtcggt
     3421 cagcgtaccc tcgagcccgt agccgacctc gatgcgcacc ttgtgctcgg ctggggcgac
     3481 cagaagaaga acgccgttgt tcttcttcgc ctcgccgagc ttccagaaac ggaacagctg
     3541 attagcgtaa gtctcgatat cgctgccttg cagcgacttg accgtggcga cgaccagctg
     3601 aatgccggat ttgtcttcga ggcctttgag catctcctcg agatcgttgc gggtgcccga
     3661 cttgatgacg ccggcctggt cgacgatgcg gccagtgagc ggcggaaaat caaacgccaa
     3721 ggcggagagc gccaggcaga gaacgaggcc ggcagccaag catgccgcga tcgccgtgcg
     3781 caccagcgga cccggccttc ggccggccgg gcccgcctct cgggaacgac ggccgaatgc
     3841 catcaaaact tcaccggcgg cggcttgtcc gaaccctccg tcgccgtgaa ttccgccatc
     3901 ggcttgttgc cggagaagaa gaacttcgcc cacagcaagg tcgggaaggt gcgcagcgcc
     3961 gtattgtagg cgcgcactgc gtcgatgtag tcgcgtcgcg ccaccgcgat gcggttctcg
     4021 gtgccctcga gctgcgactg cagcgccagg aaattggcgt tggatttgag gtcgggatag
     4081 ttctcgctca ctgcaaggag ccggcccagt gcgccggtga gttggttctg cgcgtcctgg
     4141 aaggctttga gcttgtccgg atcggtcagc tgggagacgt cgatcttggt ttgcgtcgcc
     4201 ttggcgcgcg cctcgacgac ggcggtgagg acgttcttct cctgcgcggc gtagcctttg
     4261 actgtttcca cgagattggg aatgagatcg gcgcggcgct gatactggtt ctgcacgtcc
     4321 gaccatcgcg ccttggcggt ctcttgcagt tgcgggatgg tgttgtaacc gcatcccgaa
     4381 agggtcaatc cgaggccaat tacggccaga aacacgcgga t
//
LOCUS       DASYNS010000126         6386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2056145, whole genome shotgun sequence.
ACCESSION   DASYNS010000126 DASYNS010000000
VERSION     DASYNS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2056145"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..174)
                     /locus_tag="VGP50_04030"
     CDS             complement(10..174)
                     /locus_tag="VGP50_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026570.1"
                     /translation="MLLDGKDMLAEDQHGGRRLEERLPARAGRHASSLTRSQAGFKSA
                     LPALSRFRAA"
     gene            complement(342..1484)
                     /gene="dnaJ"
                     /locus_tag="VGP50_04035"
     CDS             complement(342..1484)
                     /gene="dnaJ"
                     /locus_tag="VGP50_04035"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV8026571.1"
                     /translation="MAKKDFYELLGVARTASPEEIKKAYRKLAMQHHPDRNSGDKGAE
                     QTFKDINEAYDVLKDEQRRAAYDRFGHAAFEQGGRPGQAGAGAGDFGFAAGFADIFDE
                     MFGEFMGGRRGQGSNARGSDLRFNLEITLEEAFKGTQTTVRVPTQAPCEACNGSGAAA
                     GSRPVSCPSCHGAGRVRAQQGFFTIERTCPSCHGAGRVIENPCRSCSGQGRQRKEKTL
                     AVTIPSGVEDGTRIRLAGEGEAGLRGGGAGDLYIFLAVKPHRFFQRDGANIHCRVPIA
                     LPMAALGGAIEVPSVDGSRAKVNIPAGTQGGHQFRLKGKGMSVLRSAARGDMYVQVVV
                     ETPVNLTKRQQELLREFEKAGDAHRTSPESEGFFARVREFFEDLRE"
     gene            complement(1582..3522)
                     /gene="dnaK"
                     /locus_tag="VGP50_04040"
     CDS             complement(1582..3522)
                     /gene="dnaK"
                     /locus_tag="VGP50_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV8026572.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGKNAKVIENTEGMRTTPSIVAFTE
                     SGERLVGQAAKRQAVTNPEATLYGIKRLIGRRMSDPMVRKEMELVPFKIIQGDNGDAW
                     VECRSKKYAPSQISAFVLQKMKETAEAYLGEPVTEAVITVPAYFNDSQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLEKKGAGVIAVYDLGGGTFDISVLEIGDGVFEVKSTNG
                     DTFLGGEDFDKRVIDYLADEFKKEQGIDLRGDRLALQRLKEAAEKAKVELSSSMQTEV
                     NLPFITADRTGPKHLNIKLTRAKLEALVDDLIQKTIEPCRNALKDAGLKASEIQDVIL
                     VGGMTRMPKVIETVKQFFGREPNRSVNPDEVVAVGAAIQAGVLKGEVKDVLLLDVTPL
                     SLGIETLGGVFTRLIDRNTTIPTKKSQVFSTAEDNQTAVTIRVFQGEREMAADNKLLG
                     QFDLVGLPPAPRGMPQVEVTFDIDANGIVSVQARDKATGKEQQIRIQASGGLSGADIE
                     KMVKDAEAHADEDKRKRELVEAKNQAEGLAHTTERQIKEAGDKLPQGERDAVEAAIRE
                     VRSALESKDLERIRSKTQALAQAAMKIGEAAYKAQAGAGGAQASPGGAGAAGGPGGAA
                     GNEKVVDADFEEVDDQKKRGSA"
     gene            complement(3859..4431)
                     /gene="grpE"
                     /locus_tag="VGP50_04045"
     CDS             complement(3859..4431)
                     /gene="grpE"
                     /locus_tag="VGP50_04045"
                     /inference="COORDINATES: protein motif:HMM:NF010739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV8026573.1"
                     /translation="MSDEKNGATAPQDGAVDAEPEAVVQRLAAVEAELAETKDRMLRA
                     LAETENVRRRAQREREDAARYGATNFAKDLVSVADNLHRALASVPEGSAADELMRTVL
                     QGVSATERELLAAFERHGIRRIEPLGERFDHNLHQAIFEVENSGRPPGTVVQVLQPGY
                     VMHDRLLRPAMVGVAKGERPADAPRIDEVI"
     gene            complement(4454..5515)
                     /gene="hrcA"
                     /locus_tag="VGP50_04050"
     CDS             complement(4454..5515)
                     /gene="hrcA"
                     /locus_tag="VGP50_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV8026574.1"
                     /translation="MPPPAPRPVITELNERSREIFRLIVDAYVETGEPVGSRTLSRRS
                     HLDLSPATIRNVMADLEDAGLLFSPHTSAGRLPTDAGLRLFVNGLLEIGGLSEEERRS
                     IDGRLVAANKTLGQALEEASAALSGLSRCAGLVVAPKTERRSLKHIEFVPLSPERALV
                     VMVTEDGLVENRLIDVPIGTPPSTLVSAGNYLNSRLAGHTVDEAKAAITLDIETKRAQ
                     LDELTAKLVEAGLASWSGGGVGSALIVRGQANLLEDVTALGDLERLRTLFDMLETKES
                     MLRLLDATREGEGVQIFIGADNPLFGVAGCSMIVAPYANSREQVVGAIGVIGPTRINY
                     ARIIPLVDYTAKVIGKLLG"
     gene            5643..6365
                     /gene="rph"
                     /locus_tag="VGP50_04055"
     CDS             5643..6365
                     /gene="rph"
                     /locus_tag="VGP50_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEV8026575.1"
                     /translation="MPRPSGRAPHELRPVRLEPGFAKYAEGSCLARFGDTHVLCTATI
                     EEKVPPFLRNSGRGWITAEYGMLPRATETRTDREAARGRQSGRTQEIQRLIGRSLRAI
                     TKLAALGERQIRIDCDVLQADGGTRTAAITGSYVALHAALDRLVRAGTLPALPLIDAV
                     AAVSCGIHGGEPVLDLDYAEDSTAATDANFVLTGAGGIVEIQATAEAEPFSEARFATM
                     LALARSGIAELVRIQRAALGLG"
BASE COUNT          927 a         2193 c         2138 g         1128 t
ORIGIN      
        1 gcgcgagtat cacgcggcgc ggaagcggct cagtgccggc agcgcgctct tgaacccggc
       61 ttgggagcgg gtgagtgaag acgcgtgacg gccggcgcgc gccggtagtc gttcctcgag
      121 ccgccggccg ccatgctggt cctcggcgag catgtccttg ccgtccagca gcatcgtgta
      181 atagccgagc ttcgccgcct cagctgcaga accttcgtgc gtccttcgac aagctcagga
      241 tgaggggtat cgagcgcggc atctgacaga tgcccgatgc ccgtctcggt cttcctcatc
      301 ctgagcttgt cgaaggacgc acggccgttc tgcagccgcg gctattccct tagatcctcg
      361 aagaactcgc ggacccgggc gaagaagcct tcgctttcgg ggctggtgcg atgggcgtcg
      421 ccggccttct cgaattcgcg cagcagctcc tgctggcgct tcgtcaggtt gaccggggtc
      481 tcgacgacca cctggacata catgtcgccg cgcgcggccg agcgcagcac cgacatgccc
      541 ttgcccttca agcggaactg atgaccgccc tgagtgccgg cgggaatgtt gaccttggcg
      601 cggctgccat cgaccgacgg cacttcgatc gcgccgccga gcgcggccat cggcaaagcg
      661 atgggcacgc ggcaatggat gttggcgccg tcgcgctgga agaagcgatg cggcttcacg
      721 gcgaggaaga tatagagatc gcccgcgccg ccgccgcgca ggccggcttc gccctcgccg
      781 gcgagacgga tgcgcgtgcc gtcctcgacg cccgagggga tggtgaccgc gagcgtcttc
      841 tccttgcgct gccggccctg gccgctgcag gagcggcacg gattctcgat gacgcggccg
      901 gcgccgtggc aagaggggca ggtgcgctcg atggtgaaga agccctgttg cgcgcgcacg
      961 cggccggcgc cgtggcagga ggggcagctc accgggcgcg accccgcggc ggcgccgctg
     1021 ccgttgcagg cctcgcaggg cgcctgggtc ggcacgcgca cggtcgtctg cgtgcccttg
     1081 aaggcttcct cgagcgtgat ctcgaggttg aagcggagat cgctgccgcg ggcgttcgac
     1141 ccctggccgc gccggccgcc catgaactcg ccgaacatct catcgaagat gtcggcaaag
     1201 cccgcggcga agccgaaatc gccggcgccc gcgccggcct ggccgggacg cccgccctgc
     1261 tcgaaagcgg cgtggccgaa gcggtcgtat gccgcgcgcc tttgctcgtc cttcagaacg
     1321 tcatatgctt cgttgatgtc cttgaaggtc tgctccgccc ccttgtcgcc ggaattgcgg
     1381 tcgggatggt gctgcatcgc gagcttgcga taggccttct ttatctcttc gggcgatgcc
     1441 gtgcgcgcca cccccaagag ctcgtaaaag tccttcttgg ccaccgctac tctgcctcgt
     1501 ccctatctcg ccccgtcgac agccggccgc cccggcacgg gccggggcgg cgtcttcaga
     1561 ccaccgcggc gacccggcct tttaggccga gccgcgcttc ttttgatcgt cgacctcttc
     1621 gaagtcggcg tcgacaactt tctcgttgcc cgcggcgccg cccgggccgc cggcagcgcc
     1681 ggcgccaccg ggcgacgctt gtgcaccgcc ggcaccggct tgcgccttgt acgccgcttc
     1741 gccgatcttc atcgccgcct gggccagcgc ctgagtcttg ctgcggatgc gctcgaggtc
     1801 tttcgattcg agcgccgacc gcacctcgcg gatcgccgct tcgacggcgt cgcgctcgcc
     1861 ttgcggcagc ttgtcgcccg cctctttgat ctgccgctcg gtggtgtgcg ccaagccttc
     1921 ggcctggttc ttggcctcga ccagctcgcg cttacgcttg tcctcatcgg catgcgcctc
     1981 ggcgtccttc accatcttct cgatgtcggc gccagagagg ccgcccgagg cctgaatgcg
     2041 gatctgctgc tccttgcccg tcgccttgtc cctcgcctgc accgagacga tgccgttggc
     2101 gtcgatgtcg aaagtgactt cgacctgcgg catgccgcgg ggcgccggcg gcagacccac
     2161 caggtcgaac tgacccaaga gcttgttgtc cgccgccatc tcgcgctcgc cctggaagac
     2221 gcggatggtg accgcggtct ggttgtcctc ggcggtcgag aagacctggc tcttcttggt
     2281 cgggatcgtc gtgttgcggt cgatgagccg cgtgaacacg ccgcccagcg tttcgatgcc
     2341 gagcgacagc ggcgtcacgt cgaggagcag gacgtccttg acctcgccct tcagcacgcc
     2401 cgcctggatg gcggcgccga cggcgaccac ttcgtccgga ttgaccgagc ggttgggttc
     2461 cctgccgaag aactgcttca ccgtctcgat gaccttgggc atgcgggtca tgccgccgac
     2521 caggatcaca tcctggatct cggaggcctt caggcccgca tccttgagcg cgttccggca
     2581 gggctcgatg gtcttctgga tgaggtcgtc gaccagcgcc tcgagcttgg cgcgcgtcag
     2641 cttgatgttg agatgcttcg gtcccgtgcg gtccgccgtg atgaagggca ggttcacctc
     2701 ggtctgcatc gaggacgaga gctcgacctt ggccttctcg gccgcctctt tgagccgctg
     2761 cagcgccaga cggtcgccgc gcaggtcgat gccctgctcc ttcttgaact cgtcggcgag
     2821 atagtcgatg acgcgcttgt cgaagtcctc gccgcccagg aaggtgtcgc cattggtcga
     2881 cttcacctcg aagacgccgt cgccgatctc gagcaccgag atgtcgaagg tgccgccgcc
     2941 caggtcatag accgcgatga cgccggcgcc cttcttctcg aggccgtagg cgagcgcggc
     3001 cgccgtcggc tcgttgatga tgcgcagcac ctcgaggccg gcgatcttgc cggcatcctt
     3061 ggtggcctgg cgctgactgt cgttgaagta ggcggggacg gtgatcaccg cctcggtcac
     3121 gggctcgccc agataagcct ccgccgtctc cttcatcttc tgaagcacga aggcgctgat
     3181 ctgcgagggc gcgtatttct tgctgcggca ttcgacccag gcgtcgccat tgtcgccctg
     3241 aatgatcttg aaggggacga gctccatctc cttcctgacc atcgggtcgc tcatgcgccg
     3301 gccgatgagc cgcttgatgc cgtaaagcgt cgcttcggga ttggtcaccg cctggcgctt
     3361 ggccgcctgg ccgacgagac gctcacccga ttccgtgaag gcgacgatcg agggcgttgt
     3421 gcgcatgccc tcggtgttct cgatcacctt cgcattcttg ccttccatca cggcgacgca
     3481 ggaattcgtc gtgccgagat cgataccgat aactttcgcc atggccacct cttttgcagc
     3541 agggttccgc agcccgggag gcactgcgcc aaccccctag gaatggtcct tctcgtgggc
     3601 gccctcgtta agggcaaaga ttatatatga acgtccgatc cgcgctgcaa ccgcgaagaa
     3661 ccgcaaaccg gatgcgtcat acccccgcga gatatgggga gggtgggggc tgcgctgcaa
     3721 gtggaaagac cgctgtcggc cgcggctggc cagggaaaat gaaggccgcc aaaggttaaa
     3781 gaggaacgca gaggtcgcgg tggatgcgcg aaggccgcgg aggaacaatt atcatcctct
     3841 gcgctctccg cgttcccctt aaatgacctc gtcgatgcgc ggcgcgtcgg ccggacgctc
     3901 gcctttggcc accccgacca tggcgggccg cagcagccgg tcatgcatga cgtagccggg
     3961 ctgcagcacc tggaccaccg tgcccggcgg ccgcccgctg ttctcgactt cgaagattgc
     4021 ctggtggagg ttgtggtcga agcgctcccc caagggctcg atgcggcgaa tgccgtggcg
     4081 ctcgaacgcg gccagcagct cccgctcggt ggcgctgacg ccctggagca cggtgcgcat
     4141 gagctcgtcg gcggcactgc cttcgggcac gctcgccagg gcccggtgca gattgtcggc
     4201 gacgctcacc agatccttgg cgaaattggt ggcgccgtag cgcgcggcgt cctcgcgttc
     4261 ccgttgtgcg cggcggcgca cattctccgt ttcggctagg gcgcgcagca tccgatcctt
     4321 ggtctcggcg agctcggcct cgaccgccgc cagccgctgg acgacagcct cgggctcggc
     4381 gtcgaccgcg ccatcctgcg gcgccgtggc gccgttcttc tcgtcgctca tcgctttgtc
     4441 ctcggagcct gtcctaaccc agaagcttgc cgatcacttt cgccgtgtag tcgaccagcg
     4501 ggatgatccg cgcataattg atgcgcgtcg gcccgatcac gccgatcgca ccgaccacct
     4561 gctcgcggct gttggcgtag ggcgccacga tcatcgagca gccggcgacg ccgaagagcg
     4621 ggttgtcggc gccgatgaag atctgcaccc cctcgccctc gcgcgtcgcg tcgagcaggc
     4681 gcagcatcga ttccttggtc tcgagcatgt cgaagagcgt gcgcaaccgc tcaagatcgc
     4741 caagcgcggt cacgtcctcg aggaggttcg cctgaccgcg cacgatcaag gccgagccga
     4801 cgccgccgcc ggaccaggag gcgagcccgg cctcgaccag cttggccgtg agctcgtcca
     4861 gctgggcgcg cttcgtctcg atgtcgaggg tgatcgccgc cttcgcctcg tcgacggtgt
     4921 ggccggcgag ccgcgaattg aggtaattgc cggcgctgac cagggtcgag ggcggggtcc
     4981 cgatgggcac gtcgatgagc cggttctcga ccagcccgtc ctcggtcacc atgaccacca
     5041 gcgcgcgctc ggggctcagc ggcacgaatt cgatgtgctt gagcgagcgg cgctccgtct
     5101 tcggcgcgac gacgagcccg gcgcagcgcg acaggcccga aagcgcggcg ctcgcttcct
     5161 cgagcgcttg ccccagcgtc ttgttggcgg cgacgagccg gccgtcgatg ctgcggcgct
     5221 cctcctcgct taagcccccg atttcgagga gcccgttgac gaagagccgc aagccggcat
     5281 cggtcggcag ccttccggca gaggtatgcg gcgagaacag caggccggca tcctcgagat
     5341 cggccatgac gttgcggatg gtggcgggcg aaaggtcgag atggctgcgc ctggacagcg
     5401 tgcgcgagcc caccggctcg cccgtctcga cgtaagcgtc gacgatcagc cggaagatct
     5461 cgcggctgcg ctcgttgagc tcggtgatga ccggtcgagg ggcgggcggc ggcatctacg
     5521 tttcacgggc tgaaggaggc gcggaatgta ggaatgacgc catccagcgt caaccgcacg
     5581 cctgcgctgg acggcggcgg cggggagcac tagcttccgc gtcaacaagc aaaggaacgc
     5641 cgatgccacg cccgtcgggc cgcgcccctc atgagctgcg ccccgtgcgt ctcgagccgg
     5701 gtttcgccaa atatgccgaa ggctcgtgcc tcgcgcgctt cggcgacacc catgtgctgt
     5761 gcacggcgac catcgaggag aaggtgccgc ccttcctgcg caacagcggg cgcggttgga
     5821 tcacggccga atacggcatg ctgccccgcg ccaccgagac gcgcactgac cgcgaggcgg
     5881 cgcgcgggcg ccagagcggc cgcacgcagg agatccagcg cctgatcggg cgctcgctcc
     5941 gcgccatcac caagctcgcg gcgctgggcg agcggcagat ccgcatcgat tgcgacgtgc
     6001 tccaggccga tggcgggacg cgcaccgccg ccatcaccgg cagctatgtg gcgcttcacg
     6061 ccgcgctcga ccgtctcgtg cgcgcgggga ccttgccggc gctccctttg atcgatgcgg
     6121 tggccgcggt ctcctgcggc atccatggcg gcgagcccgt cctcgatctc gactacgccg
     6181 aggattcgac cgcggcgacg gacgccaatt tcgtgctcac tggcgcgggc ggcatcgtcg
     6241 agatccaggc gaccgccgag gccgagccct tctccgaggc gcgcttcgcg acgatgctgg
     6301 cgctggcacg aagcggcatc gccgagctgg tgcggatcca gcgcgccgcg ctcggcctcg
     6361 gctagcgttg ccgatcgcgc gcatgg
//
LOCUS       DASYNS010000127         1506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3928544, whole genome shotgun sequence.
ACCESSION   DASYNS010000127 DASYNS010000000
VERSION     DASYNS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3928544"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VGP50_04060"
     CDS             <1..568
                     /locus_tag="VGP50_04060"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV8026576.1"
                     /translation="NDIADFRAPRPAESRDAPSPSRRFYFGLTNFAKPIVAAVPGLAI
                     GIGCTMLLHADIVYAAPEARFRLPFVDLGLVPELGASMLVPWLVGKHRAAELLLLADF
                     FDAATAERLGFVNRILPQAELLPQATATARALARKPQEALRLTKKLLRPDMKPLLQRM
                     EEERELLAERLRAPETQAIMAGVLKPKP"
     gene            583..>1506
                     /locus_tag="VGP50_04065"
     CDS             583..>1506
                     /locus_tag="VGP50_04065"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEV8026577.1"
                     /translation="MSLLEKVEGRLSLPVIGAPLFIISVPDLVVAQSSAGVIGSMPSL
                     NARPIEQLDAWLAEINERLAAHDERNPDRPAAPFAINLIVHKSNDRLEQDLQIVVKHK
                     VPLVITSLGARPEVNQAVHSYGGHVLHDVINQRYAHSAIDKGADGLVLVSSGAGGHAG
                     ALSPFGFLQETRAWFDGVIALSGAIAHGSSVLAAEAMGADFAYVGSAFIATEEARASD
                     AYKAMLVAHGAEDIVYTNLITGVHGNYLKPSLRAAGLDPDNLPISDPGAMNFGSDRKK
                     RWKDIWGSGQGIGTVTAVVPTAALVARLRREY"
BASE COUNT          236 a          530 c          511 g          229 t
ORIGIN      
        1 aaacgacatc gccgatttcc gggcgccgcg cccggcggag tcgcgggatg ccccgtcgcc
       61 cagccggcgc ttctatttcg ggctcacgaa tttcgcgaag ccgatcgtcg ccgccgtgcc
      121 gggcctcgcc atcggcatcg gctgcaccat gctgctccac gccgatatcg tctacgccgc
      181 gcccgaggcg cgcttccgac tgcccttcgt cgatctgggc ctggtgccgg agctcggggc
      241 ctcgatgctc gtgccatggc tggtcggcaa gcaccgggcg gcggagctgc tgctgcttgc
      301 cgacttcttc gatgcggcga cggccgagcg cctgggcttc gtcaaccgca tcctgcccca
      361 ggccgagctg ctgccgcagg cgacggcaac ggcgcgcgcg ctggcgcgga agccgcagga
      421 agcgctacgg ctcacaaaga agctgctgcg ccccgacatg aaaccgctgc tccagcgcat
      481 ggaggaagag cgcgagctcc tcgccgagcg cctgcgcgca cccgagaccc aggccatcat
      541 ggccggcgtc ctgaagccga aaccgtgaga ggcgagacca gcatgtcgct cctggagaag
      601 gtcgagggcc ggctgtcgct gcccgtcatc ggcgcgccgc tcttcatcat ctcggtgccc
      661 gacctggtgg tggcgcagtc gagcgcgggc gtcatcggct ccatgccctc gctcaatgcg
      721 cggccgatcg agcagctcga cgcgtggctg gccgagatca acgagcgcct cgcggcgcat
      781 gacgagcgca acccggaccg gcccgccgcg cccttcgcca tcaatctcat cgtgcacaag
      841 tcgaacgacc ggctggagca ggaccttcag atcgtggtga agcacaaggt accgctggtc
      901 atcacctcgc tgggcgcacg gcccgaggtg aatcaggccg tgcattccta tggtggccat
      961 gtcctgcacg acgtgatcaa ccagcgctac gcccacagcg ccatcgacaa gggcgcggac
     1021 gggctggtgc tggtttcctc gggcgccggc ggccatgcgg gcgcgctttc gcccttcggc
     1081 ttcttgcagg agacgcgcgc atggttcgac ggcgtgatcg cgctctcggg cgccatcgcc
     1141 catggcagca gcgttctcgc cgccgaggcg atgggcgccg acttcgctta tgtgggaagc
     1201 gccttcatcg ccaccgagga ggcgcgggcg agcgacgcgt acaaggccat gctcgtcgcg
     1261 cacggcgccg aggacatcgt ctacaccaac ctcatcaccg gcgtgcacgg caactacctg
     1321 aagccgtcgc tgcgcgcggc gggactcgat cccgacaatc tcccgatcag cgatcccggc
     1381 gccatgaact tcggcagcga ccgcaagaag cgctggaagg acatctgggg ctcggggcag
     1441 gggatcggca ccgtgacggc ggtggtgccg acggcggcgc tagtggcgcg gctcaggcgc
     1501 gagtat
//
LOCUS       DASYNS010000128         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2993334, whole genome shotgun sequence.
ACCESSION   DASYNS010000128 DASYNS010000000
VERSION     DASYNS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2993334"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VGP50_04070"
     CDS             <1..580
                     /locus_tag="VGP50_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026578.1"
                     /translation="IFIGSTTGITETGTASFATPLVIFDATGAPISAAAGVNPASGAS
                     LVTTYVPGADSANIQLSSILAPNSSVLLAANHGHITIADINTRVLGVVGQFGSAQMFG
                     EIGGIAGPTAAQNVGKSGERDNNYRFNNCAIGSLTCIVLPEIVPITPQPVETITVLRQ
                     QQFNDPTINLLNVGDEDLVLTSEATTDSEKAQ"
     gene            583..>3498
                     /locus_tag="VGP50_04075"
     CDS             583..>3498
                     /locus_tag="VGP50_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEV8026579.1"
                     /translation="MRRITMRNPGLSKLLVGVALLALAGCQNLPPAAMVSDPTSPTAQ
                     AGTDVGKNQVGEACEYRADALRDVDLDAKRAYGVWCGTWRQPSGRIFEESETHEGAAQ
                     LMQLATASLWRSYLDQRTTCTAPAATTILDNVPAVLLQCTERNGGWPHVAIAATLGGK
                     TFLTDGVPSAVPAVEATVASLAGRPIAGTSSRSAAGALLDQRLSTQPFGSGDLDRYFG
                     LMRLGDSRNAVDDFAGAEDAFRDALAVQQKILGPTNPGLAMPLMHLALQISNQHRFGE
                     ASALFARAQQLITDATDPLLTARLDYYLAMHALNQNQIAEATAHANKAEKEFLTFVPP
                     GLEEAAMRGSPAPTARGGRGSALGSLQPLIVDPQAETAIRGVAAVWRLKSTMAYEGGS
                     FGESHKIANEARALLEISGFNPPGTVPRVVRVAALSDAGIGDLTAAERGLRESAYLFD
                     QVTPNEKPVAITLFLAGREARAHDDLDEALRQFRAGAKILRDRHLGLAENFITPFLDA
                     LFADATNHPNRATAIHAEMFEAAQLVQGNLTAQYIAKAAARLAAGDEKVSVALRQLQD
                     AELALKALFQERDTEAQKPPVLQDAKKLAQLDAAIADAEGKRDGAEAAAQAAAPSYGQ
                     LIQADASTKDVTGLLEESEAFVSVYLGQKSSFGFFVTRQGVIAYRVPLTFETASKGVE
                     HLRKTAQVDISDRGDVRIPNFDVAAAHELYQQLFAPVADKLGGIDRIVVSSSGPLLAL
                     PFDMLVTEETPAVTNGDYRKVPFLLKRFALSYVPAPQTFVRLRQIKSSSQAPEPYIGF
                     GDFKKPTNAQFAVTFPRDRCASDLDALAQLKDLPGTRDEILGVGKLLELKGQDIVLGE
                     RFTKAALEGTDLKRYRVIHLATHAFLPTELRCLSEPSILMSAPVKAESANDAFLYSGE
                     VLRLPMDADLVILSACNTAGPGGAAGESLSGLARAFFYAGSRGLLVTHWSVDDDSAEF
                     "
BASE COUNT          592 a         1254 c         1118 g          534 t
ORIGIN      
        1 gatcttcatc ggctcgacca ccggcatcac cgagaccggg acggcgagct tcgcgacgcc
       61 gctcgtcatc ttcgacgcga cgggggcgcc catctccgcg gccgccggcg tcaatcccgc
      121 cagcggcgcg agcctcgtca ccacctatgt gccgggcgcc gacagcgcca acatccagct
      181 ctcgagcatc ctcgcgccga attcttccgt gctgcttgcc gccaatcacg gccacatcac
      241 gattgccgac atcaacacca gggtcttggg cgtcgtcggg cagttcggtt cggcgcagat
      301 gttcggcgag atcggcggca tcgccgggcc gaccgcggcg cagaatgtcg gcaagagcgg
      361 agagcgcgac aacaactatc gcttcaacaa ctgcgcgatc ggctcgctca cctgcatcgt
      421 gctgccggag attgtcccga tcacgccgca gccggtggag acgatcaccg tcttgaggca
      481 gcaacagttc aacgacccga ccatcaacct gctcaacgtg ggcgacgagg acctggtgct
      541 gacctccgag gcgacgaccg actccgaaaa ggcgcaatga gcatgcggag aatcacgatg
      601 cgcaacccag gactgtcgaa actcctcgtc ggcgtcgctc tgctcgcgct tgctggatgc
      661 cagaacctgc cgcccgccgc gatggtgtcg gatccgacct ccccgaccgc ccaggccgga
      721 accgatgtcg gcaagaacca ggtcggcgag gcttgcgagt accgggccga cgccttgcgc
      781 gacgtcgatc tcgatgccaa gcgcgcttac ggcgtctggt gcggaacctg gcgccagccg
      841 agcgggcgca tcttcgagga atccgagacg cacgagggcg ctgcccagct catgcagctg
      901 gcgacggcca gcctctggcg ctcttatctc gatcagcgca cgacctgcac ggcaccggcc
      961 gccaccacca tcctcgacaa cgtgccggcc gtcctcctgc aatgcaccga gcgcaatggt
     1021 ggctggccgc atgtcgccat cgcggcgacg ctcggcggca agaccttcct gaccgacggc
     1081 gtgccctcgg cggtgcccgc cgtcgaagcg acggtggcga gccttgccgg acggccgatc
     1141 gccggcacca gctcgcgctc ggcggcggga gcgctgctcg accagcgtct ctcgacgcag
     1201 cccttcggca gcggcgatct cgaccgctat ttcggcctca tgcgcctcgg cgacagccgc
     1261 aacgccgtcg acgatttcgc cggcgccgag gacgcgttcc gcgacgcgct cgccgtgcag
     1321 cagaagatcc tcgggcccac caacccgggg ctcgccatgc cgctcatgca tctggcgctg
     1381 cagatctcga accagcatcg cttcggcgag gcgagcgcgc tgttcgctcg cgcgcagcag
     1441 ctcatcacgg atgcgaccga cccgctgctg acggcgcggc tcgactacta tctcgcgatg
     1501 cacgcgctca accagaatca gatcgccgag gcgacggccc acgcgaacaa ggctgagaag
     1561 gaattcctca ccttcgtccc gccgggtctc gaggaggcgg ccatgcgcgg cagtcccgcg
     1621 ccgacggccc gcggcggacg cggctcggcg ctcggatcgc ttcagccgct catcgtcgat
     1681 ccgcaggccg agaccgccat tcgcggcgtc gccgccgtct ggcgcctcaa atcgaccatg
     1741 gcctatgaag gcggcagctt cggcgaatcg cacaagatcg ccaatgaggc gcgggcgctc
     1801 ctcgagatca gcggcttcaa cccgccgggc acggtgccgc gcgtggtccg cgttgcggcg
     1861 ctgagcgacg ccggcatcgg cgacctgacg gccgccgaac gaggcttgcg cgaatccgcc
     1921 tatctcttcg accaggtgac gcccaacgag aagccggtcg ccatcaccct cttcctcgcc
     1981 gggcgggaag ctcgggcgca tgacgatctc gacgaggcgc tccgccagtt ccgcgccggc
     2041 gccaagatcc tgcgcgatcg ccatctgggc ctcgccgaga atttcatcac gccgttcctg
     2101 gacgccctgt tcgccgacgc gacgaatcac ccgaaccgcg cgacggccat tcacgcggag
     2161 atgttcgaag cggcgcagct cgtgcaaggc aatctgacgg cgcaatacat cgcgaaggcc
     2221 gcggcgcgtc tggcggcggg cgatgagaag gtcagtgtcg cgctgcgcca attgcaggac
     2281 gcggagctcg cgctcaaggc tctcttccag gagcgcgaca cggaagcgca gaagccgccg
     2341 gtgctgcagg acgccaagaa gctggcgcag ctcgatgccg ccattgccga cgcggaaggc
     2401 aagcgcgacg gtgccgaagc ggccgcgcaa gccgccgcgc cctcctacgg ccagctgatc
     2461 caggcagacg cctcgaccaa ggacgtgacc gggctgctcg aagaaagcga ggccttcgtc
     2521 tcggtctatc tcgggcagaa gtcgagcttc ggcttcttcg tcacgcgtca aggcgtgatc
     2581 gcctatcgcg tgccgctcac cttcgagacg gcgtccaagg gcgtcgagca tctgcgcaag
     2641 accgctcagg tcgacatcag cgaccgcggc gacgtcagga tccccaactt cgacgtggcc
     2701 gccgctcacg agctctatca gcagctcttc gcgccggtcg cggataagct cggcggcatc
     2761 gaccgcatcg tcgtttcgag cagcgggccg ctgctggcgc tgcccttcga catgctggtc
     2821 accgaggaga cgcccgccgt caccaacggc gactatcgca aagtgccgtt cctgttgaag
     2881 cgcttcgcgt tgagctatgt gccggcgccg cagaccttcg ttcgcctgcg ccagatcaag
     2941 tcgagctccc aggcgcccga gccctatatc ggcttcggcg acttcaagaa gccgaccaat
     3001 gcccagttcg cggtgacctt cccgcgcgac cgctgcgcca gcgatctcga cgcgctcgcc
     3061 caactcaagg acctgccggg cacgcgcgac gagattctcg gcgtcggcaa gctgctcgag
     3121 ctcaaggggc aggatatcgt gctcggcgag cgcttcacga aggccgcgct cgaaggaacg
     3181 gacctcaagc gctaccgcgt catccatctc gcgacgcacg ccttcctgcc gacggagctg
     3241 cgctgcctct cggagccgtc gatcctcatg tcggcaccgg tgaaggcgga gagcgcgaac
     3301 gacgcgtttc tctacagcgg cgaggttctc cggctgccga tggacgccga tctcgtcatc
     3361 ctctcggcgt gcaacacggc ggggccgggc ggcgcggcgg gcgaaagcct ctcgggactc
     3421 gcgcgcgcct tcttctacgc gggttcgcgg ggactgctgg tgacacattg gtccgtcgac
     3481 gatgattcgg cggaattc
//
LOCUS       DASYNS010000129         5746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5176454, whole genome shotgun sequence.
ACCESSION   DASYNS010000129 DASYNS010000000
VERSION     DASYNS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5176454"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VGP50_04080"
     CDS             complement(<1..194)
                     /locus_tag="VGP50_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026580.1"
                     /translation="MKLSKRHWLPCLLLGASLIAAQDADAQKAFRPELGQTLQALEKA
                     TPYQDNDGAVPPRSQYQGPL"
     gene            complement(656..1228)
                     /locus_tag="VGP50_04085"
     CDS             complement(656..1228)
                     /locus_tag="VGP50_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026581.1"
                     /translation="MSSLVQTINSALAAVGPATENRLRDLLAEFGELNLGDEDRLSVL
                     ATVIATAAATYHVRHKNVYLEAVRTWALEIAGELEPEPVRLAGVPIEGPVEEGAEILI
                     SGLDSLIEAMVQADVPIQDRLVTELAVVARLLGQHDANTIHMTLMTVSGMLADPEYQP
                     GEVITLPLRDVILPLSREVRLEALTPRGVA"
     gene            1485..2090
                     /locus_tag="VGP50_04090"
     CDS             1485..2090
                     /locus_tag="VGP50_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026582.1"
                     /translation="MVDPTNALSDRNLEATTTLSLKREPACFAFLCARAVSAPLPIDS
                     INLDLASVDYFGGRASLWATLEVRSGATNEQPTAVLGFDETFLRPFTRALIEAAASRN
                     PYRMTILYEPGETERQRVVEYVTGPGSPLREVIITPHDTCVVVSARGADDGEEETLFQ
                     LPRSDSFLRSFVKMTCQADQTLRETLIATEKLARQRVAPPR"
     gene            2366..3259
                     /locus_tag="VGP50_04095"
     CDS             2366..3259
                     /locus_tag="VGP50_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026583.1"
                     /translation="MVVRFFSHHFLAPKDSDHYNYKNNAASGRLWGRLLAPLRRRLPA
                     TLMPLMLASPGDPGMLMDPAAPAPHMTSAADSGCLSLERDGQGQVAFSSEGHSLGGCT
                     TTALPLVQKTMLGSGTLSSDLEFSNMDFGQMTAQEVALKFGIGRDLPIIGMHADLDGS
                     VGRVLGNLTPVVDEQLHATFSRPLVAHWDTGLETRLTSLSVLGPVQPTERSSLLFLRG
                     RYQLAGGQGVQQNIELKLSADSLDSTTQGPARHARADFSYEYHREANVLSIGLNAIDT
                     AQGVGPTGPSLGLNIRATRRF"
     gene            3383..4762
                     /locus_tag="VGP50_04100"
     CDS             3383..4762
                     /locus_tag="VGP50_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEV8026584.1"
                     /translation="MYAATIGGTRYDFPDLRRLLAKASPARSGDALAGVAAGSAAERI
                     AAQMALADLPLRRFLEETVIPYEDDEVTRLILDSHDAEAFAPVAHLTVGGLRDWLLSD
                     DAATDVLAALAPGLTPEMAAAVAKICRNQDLIAIAAKTCVVTRFRNTLGLKRRLSSRL
                     QPNHPTDDPKGVLASILDGLLLGSGDAVIGINPASDSPERCLALLRLVDDLRRRLDLP
                     TQSCVLAHVTTTLGLIEEGAPVDLVFQSIAGTAAANRSFGIDLALLREARDAALSLKR
                     GTLGDNVMYFETGQGSALSANAHHGCDQQTLEARAYGVARAFAPLLVNTVVGFIGPEY
                     LFDGKQITRAGLEDHFCGKLLGLPMGCDVCYTNHAEADQDDMDNLLILLATAGVTYVM
                     GVPGADDVMLGYQSTSYHDVLAVRQLLGLAPAPEFAAWLEAMGLMTAEGLVRPDALPA
                     RFAPLLGSS"
     gene            4766..5536
                     /gene="eutC"
                     /locus_tag="VGP50_04105"
     CDS             4766..5536
                     /gene="eutC"
                     /locus_tag="VGP50_04105"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HEV8026585.1"
                     /translation="MAELPRPLDPRWLTLRSATPARIGLARAGSAVTTSELLAFQRAH
                     AEARDAVHDRLDVAPLLVGLAAQGLASVALASAAGERHTYLLRPDLGRRLDDTSRESL
                     STLPRGHDLVFVLADGLSARALMRHALPLLAAVLPSFRSHAWRIGPTAVVEQGRVAIG
                     DEIGAALDAALVAMLIGERPGLSAPDSLGVYLTWAPKPGRSDAERNCLSNIRPDGMAY
                     AEAAQRLFHLATEARRRKLTGVTLKDETMPTLLSGDGD"
     gene            5533..>5746
                     /locus_tag="VGP50_04110"
     CDS             5533..>5746
                     /locus_tag="VGP50_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026586.1"
                     /translation="MKKKRFTTEAVNLSLCARAHLEQVESTLPTIVIPAKAGTQGPPA
                     RRSPWVPAFAGTTAKERFHTNRMRSST"
BASE COUNT          931 a         2041 c         1787 g          987 t
ORIGIN      
        1 aaaagcggtc cctgatactg actgcgcggc ggcacggcgc cgtcgttgtc ctggtaaggc
       61 gtcgccttct cgagcgcttg cagcgtctgc ccgagttccg gccgaaatgc cttctgcgcg
      121 tccgcatcct gggcagcgat gagcgatgcg ccgaggagaa ggcacggcaa ccagtgacgt
      181 ttcgacagct tcatgtttct tccctgcaat aggttgtatc atcacttata cgcaccattg
      241 tggatggtat atatcaccgg gagagaaagt ctagggcatt tctagccgcc gctgcctgca
      301 gctcgccgac gacgacgagc gagcgcttcg tgctgaaaac ctcagacgtc gacgccgcgc
      361 gcgaagccct agcccgccga gagcgcgaga ggggtgtttg tgcctttgca aaaccgttcc
      421 atctccgccc ctcaggggcg gagatgagtc acgcgtgatg tgtggatgcc ttagcgctct
      481 tcgcgggaga gggatgcttc agcgcacgcg gagcgggtcg accagccgca tcgccgcgcg
      541 gcccgcgagc ttgcgcgccg cggtcttgtg ctcggcatcg gcaatgaaca tgctcgccga
      601 cgttatccag cactgcctgc ccgaccagcg tcgaagaaat cggatcgcgt tcgcctcagg
      661 cgacgccgcg gggagtgagc gcctcgagcc gcacttcgcg gctcagcggc aggatcacgt
      721 cgcgcagcgg cagcgtgatg acctcgcccg gctgatattc aggatcggcg agcatgccgc
      781 tcacggtcat cagcgtcata tggatggtat tggcgtcatg ctggccgagc aggcgcgcga
      841 ccacagccag ctcggtgacg aggcggtcct ggatcggcac atcggcctgg accatcgcct
      901 cgatcagcga gtcgagaccg gagatgagga tctccgctcc ctcttcgacc gggccctcga
      961 tggggacgcc ggccagacgc accggctcgg gctcgagctc gcccgcgatc tcgagcgccc
     1021 aggtccggac cgcctcgaga tagacattct tgtgacgcac atgataggtg gcggccgccg
     1081 tggcgatgac cgtcgccagc accgacaggc ggtcttcatc gccgaggttc agctcgccga
     1141 actcggccag gagatcgcgc agccggttct cggtcgccgg cccgaccgcg gccagcgcac
     1201 tgttgatggt ttggaccaaa gagctcatta ccgccggccc tccgccagat gccgaagcga
     1261 tgatggcggc cgagtcttaa aaattggttg ctatttctgg caaactctaa tgaaaccctt
     1321 gcgattattg tactcttcga aatcaccgcg cagtttccaa ggtccctctg gcactgccgg
     1381 ccccgttatc taaactttaa ccagcatcag cgagagtgag ggaaggacgg gggggaggca
     1441 gcaagcggaa tcccggtccg acgaacaggt cgaggtcccc gtccatggtt gatcccacaa
     1501 acgccctttc ggaccgcaat ctcgaagcca ccaccaccct ttcgttgaag cgcgagcccg
     1561 cttgcttcgc gttcctttgc gcccgcgccg tctcggcgcc gcttcccatc gactcgatca
     1621 atctcgacct cgcttcggtc gactatttcg gcggccgcgc ctcgctctgg gcgacgctcg
     1681 aagtgcgctc cggtgccacc aacgagcagc cgacggcggt cctcggattc gacgagacgt
     1741 tcctccgccc gttcacgcgc gcgctgatcg aggctgcggc gagtcgcaac ccctaccgca
     1801 tgaccatcct ctacgagccg ggcgagaccg agcgccagcg cgtcgtcgaa tatgtgacgg
     1861 gtccgggctc gccgctccgc gaggtgatca tcacgccgca cgatacctgc gtcgtcgtgt
     1921 ccgcgcgcgg cgccgatgac ggcgaggaag agacgctctt ccagctgccg cgcagcgact
     1981 cctttttgcg ctccttcgtc aagatgacct gccaggccga ccagactctc cgcgagaccc
     2041 tgatcgcgac cgagaagctc gcgcgccagc gcgtggcgcc gccgcggtag atcacgctcg
     2101 gttcaagaag aatcattttt cggcgcagag ccacattctg ctcggtgcgg ctcccgcgat
     2161 tgttgcgcaa tcgctgacgc cgccgcggac gacgcttcgc gtcgccgagg catcgccgac
     2221 aaatcgccca aaattcgata attaagtgcg ccggcgggca atctctcatt aattagccgc
     2281 gcctaccctc ttcatcaccg gaagtaattc cggccgccga ttattatcgg ttatttctcc
     2341 tctcttttta ttttgatcgg gagggatggt ggtacgtttc ttttctcatc attttttggc
     2401 gccaaaagac agcgatcact acaattataa gaacaatgct gcatcgggcc gcctctgggg
     2461 ccgcctcctg gcgccgctgc gccgccgcct gccggcgacg ctgatgccgc tcatgctggc
     2521 gtcgcccggc gatccgggca tgctcatgga cccggcggcg ccggcgccgc atatgaccag
     2581 cgcggccgac agcggctgtc tctcgctcga gcgcgacggc caaggccagg tcgccttctc
     2641 gtcggagggg cacagcctcg gcggctgcac cacgaccgcg ctgccgctcg tgcagaagac
     2701 gatgctgggc tcgggcaccc tctcgagcga tctcgaattc agcaatatgg atttcggcca
     2761 gatgacggcc caggaagtgg cgctcaaatt cggcatcggc cgcgacctgc cgatcatcgg
     2821 catgcatgcg gatctcgatg gcagcgtcgg ccgcgtgctc ggcaacttga cgccggtggt
     2881 cgatgagcag ctccatgcga ccttcagccg cccgctcgtc gcccattggg acaccggcct
     2941 cgagacgcgg ctcacctcgc tgagcgtgct cggcccggtg cagccgaccg agcgcagcag
     3001 cctgctcttc ttgcgcggcc gctaccagct cgcgggcggc cagggcgttc agcagaacat
     3061 cgagctcaag ctctcggccg acagcctcga ctcgacgacg caagggccgg cgcgccatgc
     3121 gcgcgccgat ttcagctacg agtatcaccg cgaggccaac gtcctctcga tcggcctcaa
     3181 cgccatcgat actgctcaag gcgtcggccc gaccggtcct tcgctcggcc tcaacatccg
     3241 ggccacaaga cgattctagg cgccactgcg cctagaatcg tcttggcggc gtcagcggtt
     3301 tgcggagcaa accgcgggag ccgcaccggg caaaatctgg ctccctctcc cgctctcgcc
     3361 agcgaaggtt cataatcgcc ccatgtacgc tgcgacgatc ggcggcacgc gctacgactt
     3421 ccccgatttg cgccgtctcc tggcgaaagc ctcgcccgcg cgctcgggcg acgcgctcgc
     3481 cggcgtcgcg gccggaagcg cggccgagcg catcgccgcg cagatggcgc tcgccgacct
     3541 gccgctccgc cgcttcctcg aagagaccgt cattccctat gaggacgacg aggtgacccg
     3601 cctcatcctc gacagtcacg acgcggaagc cttcgcgccg gtcgcgcatc tgaccgtcgg
     3661 cggcttgcgc gactggctgc tgtcggacga cgcagccacg gacgttctcg ccgccctggc
     3721 gcccggcctg acgccggaga tggccgccgc cgtcgcgaag atctgccgca accaggacct
     3781 tatcgccatc gccgccaaga cctgcgtcgt cacgcgcttt cgcaacacgc tcggcctgaa
     3841 gcggcggctc tcttcgcgcc ttcagcccaa tcatccgacc gacgacccga agggcgtgct
     3901 cgccagcatc ctcgacggcc tgctcctggg ttcgggcgat gccgtcatcg gcatcaaccc
     3961 cgcgagcgac agcccggagc gctgcctggc gctcctcaga ctcgtcgacg atctgcgccg
     4021 gcgtctcgac ctgccgacgc agtcctgcgt gctcgctcat gtcacgacca cgctcggcct
     4081 catcgaagag ggcgcgcccg tcgacctcgt ctttcagtcg atcgccggga ccgcggccgc
     4141 caaccgcagc ttcggcatcg acctcgccct gctccgcgag gcgcgcgacg ccgcgctctc
     4201 gctgaagcgc ggcaccctcg gcgacaatgt catgtatttc gagacgggac agggcagcgc
     4261 gctctcggcg aacgcgcatc acggctgcga tcagcagacg ctcgaggccc gcgcctatgg
     4321 cgtggcgcgc gctttcgcgc cgctgctcgt caacaccgtc gtcggcttca tcggccccga
     4381 atatctcttc gacggcaagc agatcacgcg cgccgggctc gaggatcatt tctgcggcaa
     4441 gctgctcggc ctgccgatgg gctgcgacgt ctgctacacc aaccacgccg aggccgatca
     4501 ggacgacatg gacaatctcc tgatcctgct tgcgaccgcc ggcgtgacct atgtcatggg
     4561 cgtgcccggc gccgacgacg tcatgctggg atatcagagc acctcctatc acgacgtgct
     4621 ggcggtgcgg cagctcctcg gcctggcgcc ggcgcccgaa ttcgcggcct ggctcgaagc
     4681 gatgggcctg atgacggccg agggcctggt gcgccccgac gcgctgcccg cgcgcttcgc
     4741 gccgctgctc gggagctcct gagcgatggc ggagctgccg cgtccgctcg atccgcgctg
     4801 gctcacgctg cgaagcgcga cgcccgcgcg gatcggcctg gcgcgggccg gctctgccgt
     4861 gacgacaagc gagcttctcg ctttccaacg cgcccatgcg gaggcgcgcg atgcggtgca
     4921 tgaccggctc gatgtcgcgc cgctgctcgt gggactcgcc gcgcaagggc tggcatcggt
     4981 cgcgctcgcc agcgcggccg gcgagcgcca cacctatctg cttcgtcccg atctcggccg
     5041 gcggctcgac gacacctcgc gcgagagcct tagcacactg ccgcgcggtc acgacctcgt
     5101 cttcgtgctc gccgacgggc tttcggcgcg cgcgctcatg cgtcatgcgc tgccgctgct
     5161 cgccgccgtg ctgccgtcgt tccgcagcca tgcctggcgc atcggcccga ccgcggtggt
     5221 cgaacaaggc cgcgtcgcca tcggcgacga gatcggcgcc gcgctcgacg cggcgctggt
     5281 cgccatgctc atcggcgagc gcccgggcct ctccgcaccc gacagcctcg gcgtctatct
     5341 cacctgggcg cccaagcccg gccgcagcga cgccgagcgc aactgccttt cgaacatccg
     5401 ccccgacggc atggcctacg ccgaggccgc gcagcggctc ttccatctcg cgacggaagc
     5461 gcggcggcgc aagctcaccg gcgtcaccct caaggacgag acgatgccca cgctgctttc
     5521 gggcgacgga gattgaagaa gaaaagattc accacagagg cggtgaatct ttctttgtgt
     5581 gctagagcgc atcttgagca agtggaatcg acacttccta cgatcgtcat tcccgcgaaa
     5641 gcgggaaccc aggggccgcc ggcacgccgc tcgccctggg tccccgcctt cgcggggacg
     5701 acggcgaagg aacggttcca cacgaaccgg atgcgctcca gcacac
//
LOCUS       DASYNS010000130         4142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_184789, whole genome shotgun sequence.
ACCESSION   DASYNS010000130 DASYNS010000000
VERSION     DASYNS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184789"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /gene="tcmP"
                     /locus_tag="VGP50_04115"
     CDS             complement(<1..385)
                     /gene="tcmP"
                     /locus_tag="VGP50_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="three-Cys-motif partner protein TcmP"
                     /protein_id="HEV8026587.1"
                     /translation="MVDKGFFAERSDESEVKARIVEKYFDAWGRVMVAVAKKSGRNRK
                     LAYIDLYAGPGRYQDGSASTPLLVLQRAIANPDLCAMLATLFNDMDEDNSHTLANEIA
                     KLPGIERLKHKPVVFNDEVGPEAADT"
     gene            complement(603..1895)
                     /gene="odhB"
                     /locus_tag="VGP50_04120"
     CDS             complement(603..1895)
                     /gene="odhB"
                     /locus_tag="VGP50_04120"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEV8026588.1"
                     /translation="MASEIKVPTLGESVTEATVARWLKKVGDSVAMDDPLVELETDKV
                     TLEVNAPAAGTLAEIIVGEGANVAVGALLGRIGEGAAKATPAAKPQQAQAEARAQAQA
                     PSPGVAAAAASAASAGGNVALLERSGPAVRKLVEENRLDPAKIPATGKDGRLTKGDVL
                     TQLERGAAASAPAATMAAAKPAPAPQAPAAPQPPRALAPREERVRMTRLRRRIAERLK
                     EAQNTAAMLTTFNEVDMSEAMALRERYRDTFEKKYGVRLGYMSLFAKACIVALKEIPA
                     VNAEIDGGDLIYKNHYDIGVAVGTEQGLVVPVVRDADKLGFAEIEKTIAELGRRARDG
                     KLSLEDLSGGTFTISNGGIYGSLNSTPILNPPQSGILGMHKIQQRPVVVKDRIEARPM
                     MYLALSYDHRVVDGREAVTFLVRVKECIENPERMLFEI"
     gene            complement(2019..>4142)
                     /locus_tag="VGP50_04125"
     CDS             complement(2019..>4142)
                     /locus_tag="VGP50_04125"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEV8026589.1"
                     /translation="PALEAILRRGAELGISEFVIGMPHRGRLNVLTNVMGKPFAALFS
                     EFQGNPANPADVQGSGDVKYHLGTSTDREFDGKTVHLSLTANPSHLEAVNPVVLGKVR
                     AKQRQRGDTARDEVVGLLMHGDAAFCGQGLVAESLELSELKGYRTGGTIHFVVNNQIG
                     FTTSPSYARSSPYPSDVGKIIQAPIFHVNGDDPEAVVHVSRIATEFRQRFKKDVIVDI
                     FCYRRHGHNETDEPAFTQPIMYRAIAERPTTRQLYAQRLAAEGLVSEAESEAMIADFT
                     KHLDGQFEAAKSYRPNKADWLEGAWSGLKVASGDLRRGETAVPLEVLQEVGRSLTTVP
                     EGFDLNRKIARQLEAKRKALESGEGIDWSTGEALAFGTLCGEGTFVRLSGQDSGRGTF
                     SQRHAVLVDQQTEDRYIPLNHVRDGQAAFEVIDSPLSETAVLGFEYGYSLAEPHALVL
                     WEAQFGDFVNGAQVIIDQFLSSGEAKWLRMSGLVMLLPHGYEGQGPEHSSARLERFLQ
                     LCGEDNLQVCNLTTAANYFHALRRQVRRDFRKPLVITTPKSLLRSAEAAAKLSELGPG
                     TFFRRTIDETDPLVEDQKVMRVVLCSGKVYYDLRQARRERKIRDVAIVRVEQLYPFPA
                     KALADSIGRYKYADVVWCQEEPQNMGAWNFVDRRIEQVLAGLDGKAKRARYVGRPEAA
                     STATGLLKRHNAEQAKLVDEALSII"
BASE COUNT          676 a         1329 c         1371 g          766 t
ORIGIN      
        1 aagtatcggc agcttccggc ccaacttcat cgttgaaaac aacaggcttg tgtttgagcc
       61 gctcaatgcc aggcaatttc gctatctcgt tcgccaaggt atgcgagtta tcctcgtcca
      121 tatcattgaa cagcgtcgcc agcatggcgc aaagatcagg gtttgcgata gcgcgctgca
      181 gaactaagag aggcgttgac gcggaaccat cctggtaccg ccctggtccc gcatatagat
      241 cgatgtaagc gagctttcga tttcgcccgc ttttcttggc gaccgcaacc atgacgcgtc
      301 cccacgcatc gaaatacttc tccacgatcc gggctttaac ctcggattcg tcggatcgtt
      361 cagcgaaaaa gcccttgtcc accatgggag agctttcaac agcacacgcc atagtagtgc
      421 acccttctcg gttccgcgac gagaacatat catgaactgt aaagaaaaac aaaggctttg
      481 aagcccgtgt cagcagttgg gctccggcgg cagccgggtg cgactgagtt cgacaacggg
      541 ctctgcgcaa atgctacgcg agcttgagtt gcagtcgggt gaactccgta gctactgcgc
      601 cctcagatct cgaagagcat gcgctcgggg ttttcgatgc attccttgac gcgcaccagg
      661 aaggtcacgg cctcgcggcc gtcgacgacg cggtgatcgt aggagagcgc cagatacatc
      721 atggggcgcg cctcgatgcg gtctttcacc accaccggcc gctgctgaat cttgtgcatg
      781 ccgagaatgc cggattgcgg cgggttgagg atcggcgtcg agttgagcga gccatagatc
      841 ccgccgttgg aaatggtgaa ggtgccgccc gagagatcct cgaggctgag cttgccgtcg
      901 cgggcgcggc ggccgagctc ggcgatggtc ttctcgatct cggcgaagcc gagcttgtcg
      961 gcatcgcgca ccaccggcac gaccaggccc tgctcggtgc cgacggcgac gccgatgtcg
     1021 taatggttct tgtagatgag gtcgccgccg tcgatctcgg cgttgaccgc gggaatctcc
     1081 ttgagcgcga cgatgcacgc cttggcgaag agcgacatgt agccgaggcg cacgccgtat
     1141 ttcttctcga aggtgtcgcg atagcgctcg cgcagcgcca tcgcttcgct catatccacc
     1201 tcgttgaaag tggtgagcat cgcggcggtg ttctgcgcct ccttgaggcg ctcggcgatg
     1261 cggcggcgca gccgcgtcat gcgcacgcgc tcctcgcgcg gcgccagcgc ccggggcggc
     1321 tgcggcgcgg ccggggcctg cggcgcgggc gcgggcttcg ccgcggccat cgtcgccgcc
     1381 ggcgcggacg cggcggcacc gcgctcgagc tgcgtcagca catcgccctt ggtcaggcgg
     1441 ccgtccttgc cggtggcggg aatctttgcc ggatcgagcc ggttctcctc gaccaacttg
     1501 cgcaccgcgg gccccgagcg ctcgagcaac gccacgttgc cgccggcaga ggcagccgaa
     1561 gcggccgcag ccgcgacacc cggggacggg gcctgcgcct gtgcccgcgc ctcggcctgc
     1621 gcctgttgcg gcttggcggc gggcgttgcc ttggcggcgc cctcgccgat gcggccgagc
     1681 aatgcgccga cggcgacatt ggcgccttcg ccgacgatga tctcggcgag cgtgccggcc
     1741 gcgggtgcgt tcacttcgag cgtcaccttg tcggtctcga gctcgaccag cggatcgtcc
     1801 atggcgacgc tgtcgccgac cttcttgagc cagcgcgcca cggtcgcttc ggtcaccgac
     1861 tcgcccagcg tgggcacttt gatctcgctc gccatgtccc cgctccctag gattcaccac
     1921 agagacgcag aggcacagag aagaatgatc gacgcgcgac gcgcgctatt ttctttcctc
     1981 tgtgtctctg tgtctctgcg gtcaatcttc ttactcgctc agattatcga cagcgcctcg
     2041 tcgaccagct tcgcctgctc ggcgttgtgc cgcttcagca atcccgtcgc cgtcgacgcg
     2101 gcctcggggc ggccgacata gcgggcccgc ttggctttgc cgtcgaggcc ggccagcact
     2161 tgctcgatgc gacggtcgac gaagttccag gcgcccatat tctgcggctc ttcctggcac
     2221 cacacgacgt ccgcgtattt gtagcggccg atcgaatcgg cgagcgcctt cgccgggaag
     2281 ggatagagct gctcgacgcg gacgatggcg acgtcgcgga tcttgcgctc gcgccgcgcc
     2341 tgccgcaggt cgtaatagac cttgcccgag cacagcacga cgcgcatcac cttctggtcc
     2401 tcgaccagcg ggtcggtctc gtcgatggtc cggcggaaga acgtgccggg cccgagctcg
     2461 ctgagcttcg ccgctgcctc ggcgctgcgc aggagcgatt tcggcgtcgt gatgaccagc
     2521 ggcttgcgga agtcgcggcg gacctgccgg cgcaacgcat ggaaatagtt ggcggcggtg
     2581 gtcaggttgc agacctggag attgtcctcg ccgcagagct ggagaaagcg ctcgagccgc
     2641 gccgaggaat gctcggggcc ctgcccttca tagccatgcg gcagcagcat cacgaggccc
     2701 gacatgcgca gccatttcgc ttcgcccgag ctcaggaact ggtcgatgat gacttgggcg
     2761 ccgttgacga aatcgccaaa ctgcgcctcc cacagcacca gcgcatgggg ctcggcgagg
     2821 ctgtagccgt attcgaatcc cagcaccgcc gtttccgaca aggggctgtc gatcacctcg
     2881 aaagcggcct ggccgtcgcg cacatggttg agcgggatgt agcggtcctc ggtctgctga
     2941 tcgaccagca cggcgtggcg ctgcgagaag gtgccgcggc ccgaatcctg gcccgagagg
     3001 cgaacgaagg tgccttcgcc gcagagcgtg ccgaaagcga gcgcttcgcc agtcgaccag
     3061 tcgatgcctt cgccgctctc gagcgccttg cgcttcgcct cgagctggcg ggcgatcttg
     3121 cggttgaggt cgaagccttc gggcacggtg gtgagcgagc ggccgacctc ctgcaggacc
     3181 tcgagcggaa ccgcggtctc gccgcgccgg agatcgcccg acgccacttt gaggccgctc
     3241 cacgcgccct cgagccagtc ggccttgttg gggcgatagc tcttggccgc ttcgaactgg
     3301 ccgtcgagat gcttcgtaaa gtcggcgatc atcgcctccg attcggcctc gctgaccagg
     3361 ccctcggcgg cgagacgctg cgcatagagc tggcgcgtcg tcggacgctc ggcgatggcg
     3421 cgatacatga tcggctgcgt gaaagcgggc tcgtcggtct cgttgtggcc gtggcggcga
     3481 tagcagaaga tgtcgacgat gacgtccttc ttgaagcgct ggcggaactc ggtggcgatg
     3541 cgcgacacat ggacgacggc ttcgggatcg tcgccattca catggaagat cggcgcctgg
     3601 atgatcttgc cgacatcgga aggataaggg ctcgagcgcg cataggacgg gctcgtggtg
     3661 aagccgatct gattgttcac gacgaagtgg atggtgccgc cggtgcgata gcccttgagc
     3721 tccgagagct cgagggattc ggcgacgagg ccctggccgc aaaacgcggc gtcgccatgc
     3781 atcaggaggc cgaccacttc atcgcgcgcg gtgtcgccgc gctggcgctg cttcgcccgc
     3841 accttgccca gcaccaccgg gttcaccgcc tcgagatgcg agggattggc ggtcagcgag
     3901 agatgcacgg tcttgccgtc gaattcgcgg tcggtcgatg tgcccaaatg gtatttgacg
     3961 tcgcccgagc cttgcacgtc ggcggggttg gcgggattgc cctggaactc ggagaagagg
     4021 gcggcgaacg gcttgcccat cacgttcgtc agcacgttga gccggccgcg atgcggcatg
     4081 ccgatgacga attcactgat gccgagttcg gcgccgcgcc gcaggattgc ttccaatgcc
     4141 gg
//
LOCUS       DASYNS010000131         5610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4866165, whole genome shotgun sequence.
ACCESSION   DASYNS010000131 DASYNS010000000
VERSION     DASYNS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4866165"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1933
                     /locus_tag="VGP50_04130"
     CDS             38..1933
                     /locus_tag="VGP50_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV8026590.1"
                     /translation="MARGRAQRADDHRGCRVLGACLSTQSFIDAILALPALGRALLSP
                     DRHQIAWSWYRKAPAAEIYVAPTDGSAPPRRLTETPDDTGLLSWTPDGSALVVVEDQG
                     GSEHGQLFRLALDGRMTPLTEAAPAHFPRGGEIDASGRYLVFAANLDPATGRTIEASW
                     VLRQDLASGERHVLARPAKPNAYAPRLDRKGTRVLYTRRDLDPAGTQVWMVDIDGRND
                     HELLNFGATVKTTASWFPDSRRLLVLAERPTHKRLGIYDTETGSLAWLIDDRERDIET
                     AYAPLGSDRIVVIETRDARSRALLVDPATGAETAVELPEETLIPLGPAADGLWVARRH
                     GARQPESLLRVAIEEKGAPRILGPIADPWAGSGLTARDLTPAEDFRWRSVDGLEIQGW
                     VYRPKGPSRGLVVQVHGGPTAHSERSLSAFIQSCCAAGFTVLDPNYRGSTGFGLGFRE
                     AIRKTGWGGLEQDDIRTGIEAMMARGSAEPGRVGITGTSYGGYSAWCAITRWPPEIIA
                     AAAPICGMTDLVVDYETTRPDIRPYSEEMMGGSPTALPERYRERSPIHFVDRIRGRLL
                     IVQGANDPNVTPENVHVVEGALERAGIPYETLVFGDEGHGIRKPENLRVLYARLIDFF
                     AAAFARG"
     gene            1988..3604
                     /gene="aceB"
                     /locus_tag="VGP50_04135"
     CDS             1988..3604
                     /gene="aceB"
                     /locus_tag="VGP50_04135"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEV8026591.1"
                     /translation="MKSSSGGDKNAWPKGIEIRGPMRAGYEKILSPDALTFLAALERT
                     FGAERRQLLARRAELQAKLDGGWKPDFLAETRAVRDADWTVAAIPRDLLDRRVEITGP
                     VDRKMVINALNCGANVYMADFEDASTPTWDNLIEGQINLIDAVRRTITYDDPQSRKHY
                     ALNPKTATLLVRPRGWHLPEAHLLVDGAPMSGPLFDFGLFFFHNARELVKRGSGPYFY
                     LPKIESHLEARLWNKVFAEAQEALALPKGTIRATVLIETIMAAFEMDEIIHELKDHMG
                     GLNCGRWDYIYSFIKKFAQDPTAVLPDRAQVTMTTHFLQSYVRLLVKTCHRRNIHAMG
                     GMAAQIPIKNDPAANDAAMAKVRADKEREAGEGHDGTWVAHPGLVALAKDVFDKAMPG
                     PNQIARKRQDVHVTAADLLKVPTGAITEAGLRGNINVGLGYVESWLRGVGCVPLYNLM
                     EDAATAEISRSQVWQWIRHQAKLADGRVVDRALCRKILDEELKKLKEGAPAGERYDDA
                     AKLFGDMIEAERFVEFLTLPAYEQLVQEGQ"
     gene            complement(3829..5364)
                     /locus_tag="VGP50_04140"
     CDS             complement(3829..5364)
                     /locus_tag="VGP50_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV8026592.1"
                     /translation="MLAMAQHEHPVFKCWRVQLLRPWSFKITIRRGGRIAVYLQIAHA
                     LIEEIRRGRLAPGNALPGTRELADSLDINRKTVVEAYDELAAQGWVKTDRTRGTFVSS
                     ELPVLEEKNGTLRKPPPARMPDRPDYRLVGTAPNIPAFLPEKDLLIFDDGAPDTRQVP
                     VAALARAYRSALTLLAQRNRLGYGDPRGALALREAVSTMLNVDRGLSTTPDNICLTRG
                     SQMAIYLTTRILAGPRDCVAMEELSYPPARESFRAAGAEVLSIGLDESGMRIDELEET
                     CRKKRVRAVYVTPHHQFPTTVLLKPDRRLRLLALAEQFGFAVVEDDYDHEFHFVHRPM
                     LPLASFDPWGKVVYIGSMSKLLTPSLRLGYIAAPKSFVDRAAAEIMMIDRQGDPATEL
                     AVAQLIADGEIHRHTRKAMRLYTERRLRMETLLAERFVGRVEFTLPDGGLAIWMKFTE
                     KVDMEGLVRTARAKGVMILPGNAFTISGRSIRATRLGFASMNAAELDSATRRLRQALG
                     AVA"
BASE COUNT         1019 a         1866 c         1831 g          894 t
ORIGIN      
        1 atggcgaagc cgtctgggcc ctgcccgccc ttcaggagtg gctcgcggcc gcgcgcaacg
       61 agccgatgat catcgaggat gccgagttct aggagcctgt ctgagcaccc aatccttcat
      121 cgacgcgata ctcgcgctcc cggcgcttgg ccgagcgctg ctgtcgcccg accgccacca
      181 aatcgcctgg agctggtatc gcaaggcgcc ggcggcagag atctatgtcg cgccgacgga
      241 cggctcggcg ccgccgcgcc gcctgaccga gacaccggac gacaccggcc tgctttcttg
      301 gacgcccgac gggtccgccc tggtggttgt cgaggaccag ggcggcagcg agcatggcca
      361 gctcttccgc ctcgcgctcg acggacggat gacgccgctc accgaggcag caccggcgca
      421 tttccccaga ggcggcgaga tcgacgccag cggacgctac ctcgtcttcg ccgccaatct
      481 cgatccagcg acgggacgga cgatcgaggc gagctgggtg ctgcgccagg atctcgcgag
      541 cggcgagcgg cacgtgctgg cgcggccggc aaaacccaac gcctatgccc cgcggctcga
      601 ccgcaagggc acgcgcgtgc tctatacccg tcgcgacctc gatccagccg ggactcaggt
      661 gtggatggtc gacatcgatg gccgcaacga ccacgagctc ctcaatttcg gcgcaacggt
      721 gaagacgacg gcgagctggt tccccgacag ccgccgcctg ctggtgctcg cggagcggcc
      781 gacccataag cggctcggca tctatgacac ggagaccggc agcctcgcct ggctgatcga
      841 cgatcgcgag cgcgacatcg agacggctta cgcaccgctc gggagcgacc gcatcgtcgt
      901 catcgagacg cgcgacgccc gctcgcgggc gctgctggtc gacccggcca cgggagccga
      961 gaccgccgtg gagctgcccg aggagacgct cataccgctg ggaccggcgg ccgacgggct
     1021 ctgggttgcc cgccgtcacg gcgcgcgtca gcccgaaagc ctgctgcgcg ttgccatcga
     1081 ggagaagggc gcgccgcgca ttctcggtcc catcgccgac ccctgggccg ggagcggcct
     1141 cacggcccgc gatctgacgc cggccgaaga cttccgctgg cgctcggtcg acgggctcga
     1201 gattcagggc tgggtctatc ggccgaaagg accgtcgcgc gggctcgtcg ttcaggtcca
     1261 tggcggtccg acggcccata gcgagcgctc gctctcggcc ttcatccaga gctgctgcgc
     1321 cgccggattc accgtgctcg acccgaatta tcgcggcagc accggtttcg gcctgggctt
     1381 tcgcgaggcc atccgcaaga ccggctgggg cggcctcgaa caggacgaca tccgcaccgg
     1441 catcgaggca atgatggcgc ggggctccgc cgagccgggt cgggtcggca ttaccggcac
     1501 ctcctatggc ggctattcgg cctggtgcgc catcacgcgc tggccgcccg agatcatcgc
     1561 cgccgccgca cccatctgcg gcatgaccga cctcgtcgtc gactacgaga cgacgcggcc
     1621 ggatatccgc ccctatagcg aggaaatgat gggcggcagc ccgaccgcgc tccccgagcg
     1681 ctaccgcgag cgctcgccca tccatttcgt cgaccgcatc agaggccggc tcctcatcgt
     1741 ccagggcgcc aacgatccaa atgtgacgcc ggagaatgtg cacgtcgtcg agggcgccct
     1801 cgagcgcgcc ggcatccctt acgagacgct ggtcttcggc gatgaaggcc atggcatccg
     1861 caagcccgag aatctccgcg tgctctacgc ccggctgatc gatttcttcg ccgcggcgtt
     1921 tgcccgcggc tagccgctga cccgcggccg gtttcatgct atgagcgcga aggaggagaa
     1981 gaaatcgatg aagtcgagca gcgggggcga caagaacgcc tggcccaagg gcatcgagat
     2041 acgcggaccc atgcgggcgg gttatgagaa gattctctcg cccgacgcgc tcacctttct
     2101 cgccgcgctc gagcgcacct tcggggccga gcgccggcag ctgctggcgc gccgcgccga
     2161 gctgcaggcg aagctcgatg gcggctggaa gcccgatttc ctggccgaga cgcgagcggt
     2221 ccgcgacgcc gactggacgg tggcggcgat cccgcgcgat ctcctcgacc ggcgcgtcga
     2281 gatcaccggg ccggtcgacc gcaagatggt gatcaacgcc ctcaactgcg gcgccaatgt
     2341 ctacatggcc gatttcgagg acgcgagcac gccgacctgg gacaacctga tcgaaggcca
     2401 gatcaatctg atcgacgcgg tgcgccgcac gatcacctat gacgacccgc aaagccgcaa
     2461 gcattacgcg ctcaacccga agaccgcgac gctgctcgtg cgtccccgcg gctggcatct
     2521 gccggaggcg catcttcttg tcgacggcgc gcccatgtcg gggccgctgt tcgatttcgg
     2581 cctcttcttc ttccacaatg cccgggagct cgtgaaacgc ggcagcgggc cttatttcta
     2641 tctgccgaaa atcgagagcc atctcgaggc gcggctctgg aacaaggtct tcgccgaggc
     2701 gcaagaagcg ctggcgctgc ccaagggaac catccgcgcc accgtcctca tcgagacgat
     2761 catggccgct ttcgagatgg acgagatcat ccatgagctc aaggaccaca tgggcggtct
     2821 caattgcgga cgctgggact acatctacag cttcatcaag aaattcgcgc aagacccgac
     2881 cgccgttctg cccgaccgcg cgcaagtcac gatgacgacg catttcctgc aaagctatgt
     2941 gcgtctcctc gtcaagacct gccatcgccg caacatccac gcgatgggcg ggatggcggc
     3001 gcagatcccc atcaagaacg atcccgccgc caacgacgcc gccatggcca aggtgcgcgc
     3061 cgacaaggag cgcgaagccg gcgaaggcca tgacggcacc tgggtcgcac atcctgggct
     3121 cgtggcactc gccaaggacg tcttcgacaa ggcgatgcct gggcccaacc agatcgcgcg
     3181 gaagcgccag gacgtgcatg tgacggccgc cgatcttttg aaggtgccga cgggcgccat
     3241 caccgaggcc ggcttgcgcg gcaacatcaa tgtcggcttg ggctatgtcg aatcctggct
     3301 gcgcggcgtc ggctgcgtgc cgctctacaa tctgatggag gacgccgcca ccgccgagat
     3361 cagccgcagc caagtatggc aatggatccg ccaccaggcg aagcttgccg acggccgcgt
     3421 cgtcgaccgc gcgctctgcc gcaagatcct cgacgaagag ctcaagaaac tgaaggaagg
     3481 cgcgccggcg ggcgagcgct atgacgacgc ggcgaagctc ttcggcgata tgatcgaggc
     3541 ggagcgcttc gtcgaattcc tgacgctccc ggcttatgag caactggtgc aggaagggca
     3601 gtagcggctg cagaacggcc gtacgtcctt cgacaagctc aggatgaggg tagatcgagc
     3661 gcggcatctg cttgaggcca gtttcgttct tcctcatcct gagcttgtcg aaggacgcac
     3721 ggcggtcgtt cagtccttta tttcatccgc acatagcgca gcgggaaata attgtcggcc
     3781 ggctgcacga ggtcgacagt gtcgcgggcg gcccagaccg cgagaaattt acgcgacggc
     3841 gcccaatgcc tggcggagcc ggcgcgtcgc gctgtcgagc tcggcggcat tcatactggc
     3901 aaagcccagc cgcgtggccc ggatcgaccg ccccgagatc gtgaaagcgt tgcccggcag
     3961 gatcatcacg cccttggcgc gcgcggtgcg gacgagaccc tccatatcga ctttttcggt
     4021 gaatttcatc cagatggcca ggccgccatc aggcaaggtg aactcgaccc gtccgacgaa
     4081 acgctcggcg agaagcgtct ccatccggag tcgacgctct gtataaagcc gcatcgcctt
     4141 gcgcgtatgg cgatgaatct cgccgtcggc gatcagctgg gcgacggcca gctcggtagc
     4201 cggatcaccc tggcggtcga tcatcatgat ctcggccgcg gcgcggtcga cgaaggattt
     4261 gggcgcggcg atatacccga ggcgcagact gggcgtgagc aatttcgaca tcgagccgat
     4321 atagacgacc ttgccccatg gatcgaagct cgccaaaggc aacatcggtc ggtggacgaa
     4381 atgaaactcg tggtcgtaat cgtcctcgac gacggcaaag ccgaattgct cggccagcgc
     4441 cagcagtcgc aagcgccggt cgggcttcaa gaggacggtg gtcgggaact ggtgatgcgg
     4501 cgtcacatag acagcacgga cgcgcttctt gcggcaggtc tcctccagct catcgatgcg
     4561 catgccggac tcgtcgaggc cgatggaaag cacctcggca cccgcagcgc gaaaggactc
     4621 gcgcgccggc ggatagctca gctcctccat cgccacgcaa tcgcgcggcc cggcgagaat
     4681 gcgcgtcgtc agatagatcg ccatctggct gccgcgggtg agacagatat tgtcgggcgt
     4741 cgtgctcaag ccgcgatcga cattgagcat ggtggaaacg gcctcccgca gggccagcgc
     4801 gccgcgcgga tcgccgtagc cgagccggtt ccgctgcgcc aaaagggtca gcgccgagcg
     4861 ataggcgcga gcgagcgccg cgaccgggac ctggcgcgtg tcgggcgcac catcgtcgaa
     4921 gatcaggaga tctttttcgg gcaggaacgc cggaatattg ggcgccgtgc cgaccaggcg
     4981 gtaatccgga cgatccggca tgcgcgccgg cggaggcttc cgcagcgtcc cattcttctc
     5041 ttccaggacc ggtaactccg acgagacgaa cgtgccgcgg gtacggtcgg tcttgaccca
     5101 accttgcgcg gccagctcgt cataggcttc gacgaccgtc ttgcggttga tgtcgaggct
     5161 gtccgccagc tcgcgggtgc ccggcagggc attgccgggg gcgagcctgc cgcgacggat
     5221 ctcctcgatc agcgcatgag caatctggag gtaaaccgcg atgcgtccgc cgcggcgtat
     5281 ggtgatcttg aaactccatg ggcggagcag ctggaccctc caacatttga aaactggatg
     5341 ttcatgctgg gccatcgcca gcataacctg ctctccgggt aaagaaaaag ccggcttaaa
     5401 aagccggctt aaggggagga tcgggtcccg atatggcgcg ggaggtcgcc aagctctttc
     5461 gcaggcgggc cgcagcatgt cccagtcacg attgaagccg aattgcctca acttctacga
     5521 attgctcgcg caggctgtgg cgctgatctc gccgacgatg accgcggcgc tgatcatccc
     5581 ggtcatgtat tcgaacaccg gcgattggag
//
LOCUS       DASYNS010000132         5990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3773682, whole genome shotgun sequence.
ACCESSION   DASYNS010000132 DASYNS010000000
VERSION     DASYNS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3773682"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1314
                     /locus_tag="VGP50_04145"
     CDS             127..1314
                     /locus_tag="VGP50_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8026593.1"
                     /translation="MHSLLLQIAAVTRINLKSIPQRFWLSLSTIIAVALVVMVLLAFL
                     AMANGFQRTVKSLGADDVAVVLRAGSQSEINSGLSREQVRLIEEGPGIAKGADGKPLV
                     SAELYLVVDGIKRSTRTKANLPLRGIGEEGATARHGITIVEGRMFAPGSNEIVVGKGL
                     QREFAGFDLGETVAFGSSKWTVAGVFEADGSVFESEIWADIAVVQSLFHRDNVFQTVR
                     ARLENPPALDALKRYVEDEPRLKLDVKSEAEYFADQASRTTDLIQKLGWPLAIAMAFG
                     ALAGALNTMYSSVAARAVEIATLRAIGFGGLPAFIGTLAESLILAAIGGVVGAGATFL
                     IFDGISASTLGASFTQVVFSFHLTPALATEGVVLALAVGLIGGLFPAIRAARMPIVAG
                     LSR"
     gene            complement(1356..2000)
                     /gene="maiA"
                     /locus_tag="VGP50_04150"
     CDS             complement(1356..2000)
                     /gene="maiA"
                     /locus_tag="VGP50_04150"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEV8026594.1"
                     /translation="MKLYGFFRSSAAFRVRIALNLKGLSYEQAPVHLRRNEQFRADYR
                     AVQPQALVPALALDDGEVLIQSLAIIEYLEETQPRPPLLPGHPAARARVRALALAVAC
                     DIHPINNLRVLRYVADPLGQDAKGVETWYNHWIKLGFDSIERILAEDGQAGDFCQGDE
                     PSLADVCLVPQFFNAQRYPSFDLAPYPTIRRIAGNCLKLDAFQRALPENQPDAE"
     gene            complement(2015..3112)
                     /gene="hppD"
                     /locus_tag="VGP50_04155"
     CDS             complement(2015..3112)
                     /gene="hppD"
                     /locus_tag="VGP50_04155"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEV8026595.1"
                     /translation="MMEQADTAPGYADHWDNPMGTDGFEFIEYTAPDAAALGAAFEQL
                     GFAAVARHRSKKVTLYRQGEINFIVNAEPESFAQAFARLHGPSVCAIAFRVHDAAAAY
                     RRALALGARPVMGKVGPMELTIPAIEGIGGSLLYFVDRYGDHTIYDVDFLPLGPGDAP
                     PALVGLGAIDHLTHNVHQGRMTEWARFYERLFNFRETRYFDIEGKLTGLRSKAMTSPC
                     GKIRIPINESADARSQIAEYLAAYRGEGIQHIALATPDIYGAVETLRQRGVTFQDVPD
                     TYYEAIEARLPGHGEDLERLTRDRILIDGAPASGSGLLLQIFTDTMIGPIFFEIIERK
                     GNDGFGEGNFRALFESIERDQIRRGVLDPGA"
     gene            3357..3716
                     /locus_tag="VGP50_04160"
     CDS             3357..3716
                     /locus_tag="VGP50_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV8026596.1"
                     /translation="MSAPEWRSLAVLGRYGAMSAQEVGERTAMDKVRVSRAVTRLAGR
                     RLVTRRTDPTDRRRAILDLTAEGREIYEQIVPLALAVEAELMADLTAAERAALDAAIR
                     KLEARARPQHGDDAIEV"
     gene            3779..4243
                     /gene="aroQ"
                     /locus_tag="VGP50_04165"
     CDS             3779..4243
                     /gene="aroQ"
                     /locus_tag="VGP50_04165"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEV8026597.1"
                     /translation="MAGATILVLNGPNLNLLGVREPATYGRETLADIEEACLERAAAL
                     GLSLDFRQSNHEGQLIDWIQEARENADGIILNAGAYTHSSIAILDALKAAELPIIEVH
                     LSNIHRRESFRHISYVSAAAQGIICGFGGQGYVLALDAMARLLETETRERRS"
     gene            4252..4704
                     /locus_tag="VGP50_04170"
     CDS             4252..4704
                     /locus_tag="VGP50_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEV8026598.1"
                     /translation="MANAKDLTVDDKLVRALAALLDETGLTEIEYAVGDHRIRVARST
                     TLAPAALAIPAAAPLSAASAEGLPAAAAHPGAVKAPMVGTAYLSPQPGAPAFVKVGDS
                     VREGESLLIIEAMKVMNQIPSPRAGRVAQILVADGAPVEYGQALIVLE"
     gene            4714..>5990
                     /gene="accC"
                     /locus_tag="VGP50_04175"
     CDS             4714..>5990
                     /gene="accC"
                     /locus_tag="VGP50_04175"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV8026599.1"
                     /translation="MFEKVLIANRGEIALRIHRACREMGIRTVAVHSTADDNAMHVRL
                     ADESVCIGPPRARDSYLNIPAILSAARITGADAIHPGLGFLAENANFAAMVEEHGFTF
                     IGPTPEHIRLMGDKVAAKRAAIELGIPVVPGSDGGVADVAAATETAARIGYPVLVKAS
                     GGGGGKGMRVAQSADELKNAVPAAQNEATAFFGNPEVYLERYLAHPRHIEVQILGDGQ
                     GGVIHLGERDCSLQRKHQKLLEETPSPALNATERQRLGRVVTDAMKKLGYKSAGTVEF
                     LYENGEFFFIEMNTRIQVEHAISEMVSGIDLVREQIRIAAGAQLGYRQDDVKLAGHAI
                     ECRINAENPVTFAPSPGCVTSYHVPGGMGVRIDSALYQGYTVPPQYDSLVAKLVVHGS
                     SRNECLMRLRRSLGEYVIAGIETTIPLHQRLVA"
BASE COUNT         1008 a         2016 c         1986 g          980 t
ORIGIN      
        1 atctcgctgc cgctcatact gaccgggatt gcgctgatgc ttgggctggg gatcgtcacc
       61 gggctcgttc cggcgctcaa cgcgatgcgg ctgaagatcg ccgcggcact ggggcggagc
      121 tgacccatgc actcgctgct actgcagatc gccgccgtca cgcgcatcaa tctcaagagc
      181 atcccgcagc ggttctggct gtcactctcg accatcatcg ccgtggcgct cgtggtcatg
      241 gtgctgctcg ccttcctcgc catggccaac gggtttcaac gcacggtgaa gagtttgggt
      301 gccgacgacg tcgcggtcgt attgcgcgcg ggctcgcaat cggagatcaa cagcggcctg
      361 tcgcgcgagc aggtccggct catcgaggaa gggccgggca tcgccaaggg cgcggacggc
      421 aagccgctgg tctctgccga actctatctc gtggtcgacg gcatcaagcg ctcgacccgc
      481 accaaggcca atctgccgct gcgcggcatc ggcgaggaag gcgcgacggc gcggcatggc
      541 ataacgatcg tcgaaggccg gatgtttgct cccggcagca acgaaatcgt cgtcggcaag
      601 ggcctgcagc gcgagtttgc cggcttcgat ctcggcgaga cggtcgcgtt cgggtccagt
      661 aagtggaccg tcgccggtgt tttcgaggcg gacggcagcg tgttcgaatc ggagatctgg
      721 gccgacattg cagtggtcca gagcctgttc caccgcgaca atgtctttca gaccgtccgg
      781 gcgcggctgg aaaatccgcc agcgctcgat gcgctgaagc gctacgtcga agacgagccg
      841 cgcctgaagc tcgacgtgaa gtcggaggcc gaatacttcg ccgaccaggc ctcgcgcacc
      901 acggacctta tccaaaagct cggctggccg ctcgccatcg ccatggcatt cggggcgctc
      961 gccggcgcgc tcaacacgat gtactcctcg gtcgccgcgc gggcggtcga gatcgcgacg
     1021 ctgcgcgcca tcggctttgg cgggctgccg gccttcatcg gcacgctcgc ggaatccctg
     1081 atccttgccg ccatcggtgg cgtcgtcggc gccggcgcga cgttcttgat cttcgacggc
     1141 atcagcgctt cgacgcttgg agcgagcttc acccaggtgg tcttcagctt ccacctgacg
     1201 cctgcgctcg ccacggaggg cgtcgtgctc gcgctggccg tgggcctgat cggcgggctg
     1261 tttccggcga tccgcgccgc gcgcatgccg atcgtggccg ggctcagtcg gtagcggcca
     1321 gcccatcagg gggagggcga cctacgcgtg cttgctcact cggcgtcggg ctggttctct
     1381 ggcaacgcgc gctggaacgc gtcgagcttc aggcaattgc cggcgatgcg gcggatcgtc
     1441 ggataaggcg ccaggtcgaa gctcggatag cgctgcgcgt tgaagaactg cggcacgagg
     1501 cagacatcgg ccaggctcgg ctcatcgccc tggcagaaat cgccggcttg tccgtcctcg
     1561 gcgagaatgc gctcgatgct gtcgaagccg agcttgatcc agtggttgta ccaggtctcg
     1621 acgcccttgg cgtcctggcc cagcggatcg gcgacatagc gcagcacgcg gagattgttg
     1681 atcgggtgga tgtcgcaggc gaccgcgagc gccagcgcgc gcacccgcgc ccgcgccgcc
     1741 ggatggcccg gcaacagcgg cggtcgcggc tgcgtctctt cgagatactc gatgatcgcc
     1801 agcgactgga tcagcacctc gccgtcgtcg agcgcgagcg cgggcaccag ggcttgcggc
     1861 tgcaccgccc ggtaatcggc gcggaactgc tcgttccggc gcagatgcac cggcgcctgc
     1921 tcatagctca ggcccttgag attgagggcg atccgcacgc gaaaagccgc cgaggaccgg
     1981 aagaacccgt agagcttcat ggtctacccc tccgtcacgc gccggggtcg aggacgccgc
     2041 gccggatctg gtcgcgctcg atcgattcga agagagcgcg gaaattgccc tcgccgaagc
     2101 cgtcattgcc tttgcgctcg atgatctcga agaagatcgg accgatcatg gtatcggtga
     2161 agatctgcag caacagcccg cttcccgacg ccggcgcgcc gtcgatcaga atgcggtcgc
     2221 gcgtcagccg ctcgaggtcc tcgccatggc cgggcagccg cgcctcgatc gcctcgtaat
     2281 aggtgtcggg gacgtcctgg aacgtgacgc cgcgctgccg cagcgtctcg acggcgccgt
     2341 aaatatcggg cgtggcgaga gcgatgtgct ggataccctc gccgcgatag gcggcgagat
     2401 actcggcgat ctgcgagcgc gcatcggcgc tctcgttgat gggaatgcgg atcttgccgc
     2461 agggactcgt catcgccttc gagcgcaagc ccgtcagctt cccctcgata tcgaaatagc
     2521 gggtttcgcg gaagttgaag agacgttcgt agaagcgcgc ccactccgtc atgcgtccct
     2581 gatggacatt gtgcgtgaga tggtcgatgg cgccgaggcc gaccagcgcg ggcggcgcat
     2641 caccgggccc gagcggcagg aagtcgacgt catagatggt gtgatcgcca tagcggtcga
     2701 cgaaatagag aaggctgccg ccgatccctt cgatggcggg aatggtgagc tccatcgggc
     2761 cgaccttacc catcaccggc cgcgcgccga gggccagggc gcggcgatag gctgcggcgg
     2821 catcatgcac gcgaaaggcg atggcgcaga ccgaaggccc atgcagccgg gcaaaagcct
     2881 gggcgaagct ctccggctcg gcgttgacga tgaaattgat ctcgccctgg cggtagagcg
     2941 tcactttctt cgagcgatgc cgcgccacgg cagcgaagcc cagctgctcg aaagcggcgc
     3001 cgagggccgc cgcgtccggc gcggtatatt caatgaattc aaagccgtcg gtccccatgg
     3061 ggttgtccca gtggtccgca taccccgggg ccgtgtccgc ttgctccatc atgccgagct
     3121 tatcccatcg aaacaaaggc ttgacggaaa agccaaaccc atcatcagat agcaaccaac
     3181 gattcataac accaatctta caattgggtc ttcgcgcgtg gtcataagaa atgtcggccc
     3241 agtcatcgcc tgggggtgtt ccgttcgatc ttgaacggta cctgcccttc cgtctgtccg
     3301 tcctcggcaa ccgcgtgacg cgcgcggtcg cgcgggcgta tgggccgcgt ttccacatgt
     3361 ccgcgccgga atggcgaagc ctggcggtgc tcggccgcta tggcgccatg agcgctcagg
     3421 aagttggcga gcgcacggca atggacaagg tgcgggtgag ccgcgccgtc acgcgactcg
     3481 ccggacgacg tctcgtcacg cgccgcaccg atcccacgga tcgccgccgc gccattctcg
     3541 acctcaccgc cgaggggcgc gagatctacg aacagatcgt gccgctggcg ctcgccgtcg
     3601 aagccgagct catggccgac ctgactgccg ccgagcgcgc ggcgctcgat gccgcaattc
     3661 gcaagctcga ggccagggcc cgccctcagc acggcgacga tgcgatcgag gtctagccgc
     3721 gtttgccggc ccggggttcc atgctataag gcgaccttgt gagcaacggg agcagcccgt
     3781 ggccggagcc acgatcctcg tcctcaacgg gcccaatctg aaccttctcg gcgtgcgcga
     3841 gccggcgacc tatgggcgcg agaccttggc ggacatcgag gaggcgtgcc tcgagcgcgc
     3901 cgcggcgctc ggattatcgc tcgacttccg ccagtcgaac cacgagggcc agttgatcga
     3961 ctggatccag gaggcgcgcg agaatgccga cggcatcatt ctgaacgccg gcgcctacac
     4021 ccacagctcg atcgcgatcc tcgacgcgct caaggcggcg gagctgccga tcatcgaggt
     4081 gcacctctcc aacatccatc gccgtgagag ctttcgccac atttcctatg tgagcgcggc
     4141 cgctcagggc atcatctgcg gcttcggcgg acagggctat gtgctggcgc tcgacgccat
     4201 ggcgcggctc ctcgagaccg agacacgcga gagacggagt tagcctctgg catggcgaat
     4261 gcgaaagacc tgacggtgga cgacaagctc gtccgcgcgc ttgcggcgtt gctcgacgag
     4321 accgggctca ccgagatcga atatgcggtg ggcgaccacc gcattcgcgt ggcgcggagc
     4381 accacgctgg ctcctgccgc cttggcgata cccgcggccg cgccgctctc ggcggcgtcc
     4441 gccgagggcc ttcccgccgc cgccgcgcat ccgggcgccg tcaaagcgcc catggtcggc
     4501 accgcctatc tctcgccgca gccgggcgcg cccgccttcg tcaaggtcgg cgacagcgtg
     4561 cgggaaggag agtcgctgct catcatcgag gcgatgaagg tgatgaacca gatcccctcg
     4621 ccgcgcgccg gccgcgtcgc gcagatcctg gtcgccgacg gcgcgccggt ggaatacggc
     4681 caggcgctga tcgtgctcga gtagccgccg ctgatgttcg agaaagtgct catcgccaat
     4741 cgcggcgaga tcgcgttgcg cattcatcgc gcctgccgcg agatggggat ccgcaccgtt
     4801 gcggtccatt cgaccgccga cgacaacgcg atgcatgtgc ggctcgccga cgagagcgtg
     4861 tgcatcggcc cgccgcgcgc ccgcgacagc tacctcaaca ttcccgcgat cctttcggcg
     4921 gcgcgcatca ccggcgccga cgccatccat ccgggcctgg gctttctcgc cgagaacgcc
     4981 aatttcgccg ccatggtcga ggagcacggc ttcaccttca tcggcccgac gcccgagcac
     5041 atccgcctga tgggcgacaa ggtcgcggca aagcgtgcgg cgatcgagct cggcattccc
     5101 gtcgtgcccg gctccgatgg cggcgtcgcc gatgtcgccg cggccaccga gaccgccgcg
     5161 cggatcggct atccggtgct ggtcaaggcc tcgggcggcg gcggcggcaa gggcatgcgc
     5221 gtcgcgcaga gcgccgacga gctcaagaac gccgtgcccg cggcgcagaa cgaggccacg
     5281 gccttcttcg gcaatcccga ggtctatctc gagcgctatc tcgcccatcc acgccatatc
     5341 gaggtgcaga tcctgggcga cggccagggc ggcgtcatcc atctcggcga gcgcgattgc
     5401 tcgctgcaac ggaagcacca gaaactgctc gaggagacgc cgtcgccggc gctcaacgcg
     5461 acggaacggc agcgcctcgg ccgcgtcgtg accgacgcca tgaagaagct cggctacaaa
     5521 agcgccggca ccgtcgagtt cctctacgag aatggcgagt tcttcttcat cgagatgaac
     5581 acgcgcattc aggtcgagca cgcgatctcc gagatggtga gcggcatcga tctcgtgcgc
     5641 gagcagatcc gcatcgccgc cggcgcacag ctgggctatc gccaggacga cgtgaagctc
     5701 gcgggccacg ccatcgaatg ccggatcaac gccgagaacc ccgtgacctt tgccccctcg
     5761 ccgggctgcg tcaccagcta tcacgtgccg ggcggaatgg gcgtgcgcat cgacagcgcg
     5821 ctctaccagg gctacaccgt gcccccgcaa tacgacagcc tggtggcgaa gctcgtcgtc
     5881 cacggcagct cgcgcaacga atgcctgatg cgcctgcgcc ggtcgctcgg cgaatacgtc
     5941 atcgccggca tcgagacgac gatcccgctg catcagcgcc tcgtcgccga
//
LOCUS       DASYNS010000133         2134 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2213863, whole genome shotgun sequence.
ACCESSION   DASYNS010000133 DASYNS010000000
VERSION     DASYNS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2213863"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /gene="pyrB"
                     /locus_tag="VGP50_04180"
     CDS             <1..325
                     /gene="pyrB"
                     /locus_tag="VGP50_04180"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945970.1"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV8026600.1"
                     /translation="DMAQGLKDVDIIMMLRLQTERMQGSFVPSIREYFHFFGLDYRKL
                     AAARPDALIMHPGPMNRGVEIDTEVADDIDRSLIRQQVEMGVAVRMACLDVLTRNLSN
                     HSPAK"
     gene            322..1605
                     /locus_tag="VGP50_04185"
     CDS             322..1605
                     /locus_tag="VGP50_04185"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV8026601.1"
                     /translation="MSAPILFAAARLLDPATGRDAPGDLLVADGRIAALGEVGRPRDA
                     EVIDCAGACLAPGLVDMRVELREPGAEHQESMPTGGLAAVAGGVTTMVALPNTEPAID
                     DMALVEFVARRSRDVGLARIRTYAAATKGLKGRELTEMGLLAAAGALAFTDAGRAIAD
                     ATVMRRALAYARTFDLLLMQHPEEPSLAAGGAMNEGEVATRLGLAGITPAAEVIMVER
                     DLRLVEITGGRLHFAHVTTEAAIDAIRQAKKRGLPVTCDTAPHYFALNETAVGDYRTF
                     AKVSPPLRSEWDRRAVIAGLKDGTIDAIASDHSPHDQESKRLPFASAAAGMIGLETLL
                     PLTLELYHNGSLPLLQALRALTQRPAEILRLPAGRLAVGDAADLVIFDLDRPWRIDDE
                     AFRSKSKNAPYDGRPVQGQVLRTVVAGRSVFIREG"
     gene            1611..>2134
                     /locus_tag="VGP50_04190"
     CDS             1611..>2134
                     /locus_tag="VGP50_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEV8026602.1"
                     /translation="MKPVALIGAASGWGAGFRHTEEGPAALRKLGLERWLAAGGVGAE
                     WRAMIESEKHWRAHPEVKPPETFDLVLRHAAQVADAVAAARGEGAFPVVIGGDHAVGM
                     GSWAGVARSLAYRPFGLILLDAHLDAHTIETTPSMNAHGMGMAALLGQGYPPFLALCG
                     GLLRPEHVCYVGIRS"
BASE COUNT          320 a          763 c          706 g          345 t
ORIGIN      
        1 cgacatggcg caaggcttga aggacgtcga catcatcatg atgctgcgcc ttcagacgga
       61 gcgcatgcag ggcagcttcg tgccttcgat ccgcgagtat ttccacttct tcggtctcga
      121 ctaccgcaag ctcgccgccg ccagacccga tgcgctcatc atgcatccgg gcccgatgaa
      181 ccgcggcgtc gagatcgata ccgaggtggc cgacgacatc gaccggagcc tcattcgcca
      241 gcaggtcgag atgggcgtcg ccgtgcgcat ggcctgcctc gacgtgctga cgcgaaacct
      301 cagcaatcat tcgccggcca aatgagcgcg cccatcctct tcgccgctgc gcggctgctc
      361 gatccggcaa ccggtcgcga cgcgccgggc gatctcctcg tcgcggatgg ccgaattgcc
      421 gcgctcggcg aggtcggccg cccgcgtgac gccgaggtga tcgattgcgc cggcgcttgt
      481 ctcgcgccgg ggctcgtcga catgcgcgtc gagctgcgcg agcccggcgc cgagcatcag
      541 gagtcgatgc cgacgggcgg cctcgccgcc gttgcgggcg gcgtcaccac catggtggct
      601 ctgcccaaca ccgagcccgc catcgacgac atggcgctcg tcgagttcgt ggcgcggcgc
      661 tcgcgcgatg tcggcctcgc gcgcatccgt acctacgccg ccgcgaccaa aggcctcaag
      721 ggtcgtgagc tcacggagat ggggctcctc gcggcggcgg gcgccctcgc cttcaccgac
      781 gccggaagag ccatcgccga tgccacggtc atgcggcggg cgctggctta tgcgcggact
      841 ttcgatctct tgctgatgca gcatcccgaa gagccgagcc tcgccgccgg cggcgccatg
      901 aatgaaggcg aggtcgcgac gcggctcggg cttgccggca tcacgccggc cgccgaggtg
      961 atcatggtcg agcgcgatct gcgcctggtc gagatcaccg gcggacggct ccatttcgcg
     1021 catgtcacca ccgaagccgc gatcgacgcg atccggcagg cgaagaagcg cggcctcccg
     1081 gtcacctgcg ataccgcgcc tcactatttc gcgctcaacg agacggccgt cggcgactac
     1141 cgcaccttcg ccaaggtctc gccgcccttg cgcagcgaat gggatcgccg cgcggtcatc
     1201 gccggcctca aggacggcac gatcgacgcc atcgccagcg atcattcgcc tcatgaccag
     1261 gaatcgaagc ggctgccctt cgcgtcggct gccgccggca tgatcggact cgagactctg
     1321 ctgccgctga cgctcgagct ctaccacaac ggcagcctgc cgctgcttca ggccctgcgc
     1381 gcgttgacgc agcgcccggc cgagatcctg cgccttcccg ccgggcgcct ggcggtcggc
     1441 gacgccgcgg acctggtgat tttcgacctc gatcgcccct ggcgcattga cgacgaggcc
     1501 tttcgttcca agtctaagaa cgctccctat gacggccggc ccgtgcaagg gcaagtgctg
     1561 cgcacggtgg tcgccgggcg cagcgttttc attcgggaag gctgacgtcg atgaaaccag
     1621 tcgcactgat cggcgctgca tccggatggg gcgccggttt ccgccacacc gaagaaggcc
     1681 ctgcggcgct gcgcaagctg ggcctcgagc gatggctcgc cgcaggcggc gtcggcgcgg
     1741 aatggcgcgc gatgatcgaa agcgagaagc attggcgcgc gcatcccgag gtcaagccgc
     1801 cggagacctt cgatctcgtc ttgcgtcacg ccgcgcaagt cgccgacgcc gtcgctgcgg
     1861 cgcggggcga gggcgccttc ccagtcgtca tcggcggcga tcacgcggtc ggcatgggca
     1921 gctgggcggg cgtcgcccgc agcctcgcct accgcccctt cggcctcatc ctgctcgacg
     1981 cgcatctcga cgcccacacc atcgaaacga cgccctcgat gaacgcgcat ggcatgggca
     2041 tggcggcgct gctgggtcag ggatatccgc cgtttcttgc cttgtgcggc ggcctgctgc
     2101 ggcccgagca tgtctgctat gtcggcatcc gctc
//
LOCUS       DASYNS010000134         4090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5333735, whole genome shotgun sequence.
ACCESSION   DASYNS010000134 DASYNS010000000
VERSION     DASYNS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5333735"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VGP50_04195"
     CDS             <1..559
                     /locus_tag="VGP50_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026603.1"
                     /translation="GMPLKENWNNAKAPLYSTQTLGWGGRLSGPNDGARNDIAVDGVA
                     MKNAPDSSCLSCHSTAQWNVQAHNMPSFLLPSFATPATATTPPFQTCDANGKPNPKNQ
                     GAYICSPAPGSAAWMKWFQDRAGTEPMDPGSVAADFDEVLSFKSLGLWWAAVAPSKQP
                     APLLLRNPLSAQRFNLYTGAPLPQP"
     gene            complement(587..1300)
                     /locus_tag="VGP50_04200"
     CDS             complement(587..1300)
                     /locus_tag="VGP50_04200"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV8026604.1"
                     /translation="MRVFIVHAHPEERSFNGAMTRAAHETLAAAGHDVLISDLYRMGF
                     DPVSDRRNFVTVADPDYFRQQAEEAHAASHDGFAPEIRDEHEKLFWCDVLILQFPLWW
                     FGLPAILKGWVDRVFAAGGRVYGGGKWYDRGVFAGKRAMCALTIGGPPPIYSAEGLNG
                     PIGAILFPINHGILYFTGFTVIEPFLVHAPVRLSDAGRAAELARYRERVSTLAAAPVL
                     TYPKLADYDERFVLKPGTP"
     gene            complement(1332..1898)
                     /locus_tag="VGP50_04205"
     CDS             complement(1332..1898)
                     /locus_tag="VGP50_04205"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV8026605.1"
                     /translation="MDEGFSKRSFDVAGALGGLLFFAPAMAATALAILLEDGRPILFR
                     QQRLGHRRRPFIILKFRSMRDGRVTRVGRVLRATGLDELPQFVNILRGELSAVGPRPL
                     TSADAERLGWSDAAYDFRWSVPPGLTGLAQIVGSRGARQALFLDRRYIEGRSLLLDLR
                     LIALSFAVNALGKKRMRRLLLRRQAPVA"
     gene            1978..2595
                     /locus_tag="VGP50_04210"
     CDS             1978..2595
                     /locus_tag="VGP50_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026606.1"
                     /translation="MPDLEKALAEITLIRSQMARGMEFRGLGAATLAATGGLALLGAV
                     AQARWLEEPVRHVESYLLLWLGIAAASVILIGVETVTRTARAHSGLAQEMIVAAIEQF
                     VPAVVAGALMTFVLYETAPMVLWMLPGLWQIVLGLGVFASRRSLPPAIAIAGLWYLAS
                     GLACLATAGGDHAFSPWAMGLPFGIGQMLIALIVHRHGGGADGEA"
     gene            2582..2983
                     /locus_tag="VGP50_04215"
     CDS             2582..2983
                     /locus_tag="VGP50_04215"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV8026607.1"
                     /translation="MGKPRAEKQSAARFAYEGLDRVIHERARLSVLTSLVAHPKGLRF
                     GDLKELCGLTDGNLSRHLHVLQEAQLVAIAKGFDHNRPQTLCRITARGRKRYLDYLGV
                     LEQVVRDAAAAAKRDAATDDEQDDLPGLAPA"
     gene            3017..3760
                     /locus_tag="VGP50_04220"
     CDS             3017..3760
                     /locus_tag="VGP50_04220"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF011412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HEV8026608.1"
                     /translation="MQSTSYQSEGLHVAMIMDGNGRWATRRGLPRVAGHRAGVATVRR
                     MAAAAPALGIATLTLFAFSSDNWRRPAEEVNALMGLMRSYLKSATARFVEDGVRLSFI
                     GRRDRLPRFLHEGVDAAERATADGRRLHLRIALDYSSREAITCAAVRSALAAPPPTLE
                     EFGRRIDTALSEMGGAGAVDLLIRTGGEKRLSDFLLWECAYAELWFTDIMWPDFDAAS
                     LSQALADFRRRERRFGGLAAGALAQGAAE"
     gene            3757..>4090
                     /locus_tag="VGP50_04225"
     CDS             3757..>4090
                     /locus_tag="VGP50_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026609.1"
                     /translation="MIALFRRPLRVKLILPALTEATSPYWRPIKYSLFPPLGLATIAA
                     YLDPEDEAEIVDEHVMPLDTDDAPDLVLIQVYITNAYRAYGIADGYRARGVFVALGGL
                     HVTSLPEEA"
BASE COUNT          653 a         1385 c         1411 g          641 t
ORIGIN      
        1 gggcatgccg ctcaaggaga actggaacaa cgccaaggcg ccgctctatt cgacgcagac
       61 cttgggctgg ggcggccggc tctccggccc caatgacggg gcgcggaacg acatcgccgt
      121 cgacggcgtc gcgatgaaga acgcgccgga ttcgagctgc ctcagctgcc acagcacggc
      181 gcagtggaac gtccaggcgc ataatatgcc gtccttcctg ctgccgtcct tcgccactcc
      241 ggccaccgcc acgacgcccc ccttccagac ctgcgacgcc aacggcaagc ccaatcccaa
      301 gaaccagggc gcctatatct gctcgcccgc gccgggctcc gctgcctgga tgaaatggtt
      361 ccaggaccgt gcgggcaccg agcccatgga cccgggctcg gtggcggcag atttcgacga
      421 ggtgttgtcg ttcaaatccc tcggcctatg gtgggcggct gtggccccat cgaaacagcc
      481 ggcgccgttg ctgctgcgca atccgctgag cgcgcagcgc ttcaacctct ataccggagc
      541 accgctgccg cagccgtgac gagggcgcgc cgagcgacgg agcgagctac ggcgtgccgg
      601 gtttcagcac gaagcgctcg tcgtaatcgg cgagcttcgg ataagtgagc acgggcgccg
      661 ccgcgagcgt cgacacgcgc tcgcgatagc gcgcgagctc ggccgcgcgc ccggcgtcgc
      721 tcaggcggac cggcgcgtgg acgaggaagg gctcgatcac ggtgaagccg gtgaaataga
      781 ggatgccgtg gttgatgggg aagagaatgg cgccgatcgg tccgttcagg ccctcggcgg
      841 aatagatggg cggcggcccg ccgatggtga gtgcgcacat cgcgcgcttg ccggcgaaga
      901 cgccgcggtc gtaccacttg ccgccgccat agacgcgtcc gcccgcggcg aagacgcgat
      961 cgacccagcc cttgaggatc gccggcaggc cgaaccacca cagcggaaac tgcaagatca
     1021 gaacgtcgca ccagaagagc ttctcgtgct cgtcccggat ctcgggcgca aagccgtcat
     1081 gggaagcggc atgggcttcc tcggcctgct gacggaaata atcggggtcg gcgacggtga
     1141 cgaaattgcg ccggtcggag accgggtcga agcccatgcg atagaggtcg gagatgagga
     1201 cgtcgtgccc cgcggcagcg agcgtctcgt gggccgcgcg cgtcatcgcg ccgttgaagc
     1261 tccgttcctc gggatgtgcg tggacgatga agacgcgcat gcgctgttct cgtcggacgg
     1321 cgacaacgat gtcaagcaac gggcgcctgg cggcgcagca gcagccggcg catccgcttc
     1381 ttgccgagcg cgttgaccgc gaaggagagc gcgatgaggc gcaagtcgag caacaggctc
     1441 cggccttcga tgtagcggcg atcgaggaag agcgcttgcc gcgcgccccg gctgccgacg
     1501 atctgggcaa gcccggtcaa gcccggcggc acgctccagc ggaaatcata cgccgcatcc
     1561 gaccagccga ggcgctcggc atcggccgag gtgagaggac gcggccccac tgcgctcagt
     1621 tcgccgcgca ggatgttgac gaattgcggc agctcgtcga ggccggtggc gcgcagcacc
     1681 cgcccgacgc gcgtgacgcg cccgtcgcgc atggagcgga atttgaggat gatgaaaggg
     1741 cgccgccggt ggccgaggcg ctgctggcga aagaggatgg gccggccatc ctcgagcagg
     1801 atcgccagcg ccgtcgccgc catcgctggg gcgaagaaaa gcagcccgcc cagcgccccg
     1861 gccacgtcga agctgcgttt gctgaaaccc tcgtccatgg cgcggcctcc gcataaaaaa
     1921 agctcgcctt tccttataac gtaaggtact ttgtattgca aaggacggag gcgaggcgtg
     1981 cccgatctcg agaaggcctt ggcagagatc acgctgatcc gcagccagat ggcgcggggc
     2041 atggagttcc gcggcctcgg cgcggcgacg ctggcggcga ccggcgggct ggcgctgctc
     2101 ggggcggtgg cgcaagcgcg ctggctcgag gagcccgtcc gccatgtcga gagctatctc
     2161 ctcctctggc tcggcatcgc tgcggcctcg gtcatcctca tcggcgtcga gacggtgacg
     2221 cggacggcgc gggcccattc ggggctcgcg caagagatga tcgtcgccgc catcgagcaa
     2281 ttcgtcccgg cggtggttgc gggcgcgctc atgaccttcg tcctctacga aacggcgccc
     2341 atggtgctgt ggatgctgcc ggggctgtgg cagatcgtcc tcggactcgg cgtcttcgcg
     2401 tcgcgccgct cgctgccgcc ggccatcgcc atcgccggcc tctggtatct tgccagcggc
     2461 cttgcctgtc tcgcgaccgc ggggggcgat cacgcgtttt cgccctgggc catggggctg
     2521 cccttcggca tcggccagat gctcatcgcg ctcatcgtgc atcgccatgg cggaggggcc
     2581 gatggggaag cctagggccg agaagcagag cgcggcgcgc ttcgcctatg aggggctcga
     2641 ccgggtcatc catgagcgcg cgcgcttaag cgtgctcacc tcgctggtgg cccatcccaa
     2701 gggcctgcgg ttcggggact tgaaggagct ctgcggcctc accgacggca atctgagccg
     2761 ccacctccat gtgctgcagg aagcgcagct ggtggcgatt gccaagggct tcgaccacaa
     2821 ccggccgcag acgctctgcc gcatcaccgc gcgcggacgc aagcgctatc tcgactatct
     2881 cggcgtcctc gagcaggtcg tgcgcgatgc cgcggcggcc gccaagcgcg atgccgccac
     2941 ggacgacgag caagacgacc tccccgggct cgcaccggcc tgaccactct ctcttttttt
     3001 gccaaggaac tttataatgc aaagtacttc atatcagagc gaaggcctgc atgtcgccat
     3061 gatcatggac ggcaacgggc gctgggcgac gcggcgcggc ctgccgcggg tcgcgggcca
     3121 tcgcgccggc gtcgccacgg tgcgccgcat ggccgccgcg gcgccggcgc tcggcatcgc
     3181 cacgctgacg ctcttcgcct tctcctcgga caactggcgc cgccccgccg aagaggtgaa
     3241 tgcgctgatg gggctgatgc ggtcctatct gaagagcgcc acggcgcggt tcgtcgagga
     3301 cggcgtgcgg ctcagcttca tcggacggcg cgaccggctg ccgcgcttcc ttcatgaggg
     3361 tgtcgacgcg gcagaacgag cgacggcgga cgggcggcgg cttcatctgc gcatcgctct
     3421 cgactactcc tcgcgcgagg cgatcacttg cgcggcggtg cgctcggcgc tcgcggcgcc
     3481 gccgccgacg ctcgaggaat tcggccggcg catcgatacg gcgctctccg agatgggcgg
     3541 cgccggcgcc gtcgacttgc tcatccgcac cggcggcgag aagcgcctct ccgactttct
     3601 gttgtgggaa tgcgcttatg ccgagctctg gttcaccgac atcatgtggc ccgatttcga
     3661 cgcggcgtcg ctcagccagg cgctcgccga tttccgccgg cgcgagcggc gcttcggcgg
     3721 gcttgccgcg ggcgcgctcg cccagggagc agccgaatga tcgcgctgtt ccgccggccg
     3781 ctccgcgtca agctcatcct gccggccttg acggaggcga cgagccccta ttggcggccc
     3841 atcaaatatt cgctgttccc gccgctcggg ctcgccacca tcgcggccta tctcgacccc
     3901 gaggacgagg ccgagatcgt cgacgagcat gtcatgccgc tcgatacgga cgacgcgccc
     3961 gatctcgtgc tgatccaggt ctatatcacc aatgcctacc gcgcctatgg catcgccgac
     4021 ggctatcgcg cgagaggcgt cttcgtggcg ctgggcgggc tccatgtgac ctcgttgccg
     4081 gaagaagccg
//
LOCUS       DASYNS010000135         4531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4399551, whole genome shotgun sequence.
ACCESSION   DASYNS010000135 DASYNS010000000
VERSION     DASYNS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4399551"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VGP50_04230"
     CDS             complement(<1..741)
                     /locus_tag="VGP50_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEV8026610.1"
                     /translation="MHDGAGHVFNPTILREYDIRGIVGKTLGEEDARAIGRAFAAVVR
                     DAGGKRVAIGRDGRLSSPTLEAALIEGLTKSGIDVLRIGLGPTPMLYFAVNTERVDGG
                     IMVTGSHNPADYNGFKLMLGKKSFFGADIQGLGRIAAAAPSATPTRAGRVEEKLVLDA
                     YVARVLADYEGARPLQVVWDCGNGATGEAVQRLTQRLPGRHRLLNETIDGRFPAHHPD
                     PTEAKNLVQLQEAVRADGADLGIAFDGDG"
     gene            complement(750..1622)
                     /gene="galU"
                     /locus_tag="VGP50_04235"
     CDS             complement(750..1622)
                     /gene="galU"
                     /locus_tag="VGP50_04235"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV8026611.1"
                     /translation="MAQRIRKAVFPVGGLGTRFLPATKAMPKEMLPVVDKPLIQYAVE
                     EARAAGIEEFIFVTGRSKTAIEDHFDHSYELEDTLRERGKEAQIIAVNSWMPEPGQVA
                     YTRQQVPLGLGHAVWCARNLVGEEPFAVLLADDLILAEKPCLAQMVEAQAKTDGNVVA
                     IMEVPHAHTNRYGILDVESEEGRLVKMKGVVEKPEPAKAPSNLSIVGRYILQPEVFDH
                     LERVGRGAGNEIQLTDALAELIRGNKPFHGLRFEGTRFDCGDKVGWLQANIAFALARP
                     DTGPALRDILSEYC"
     gene            complement(1770..2474)
                     /locus_tag="VGP50_04240"
     CDS             complement(1770..2474)
                     /locus_tag="VGP50_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026612.1"
                     /translation="MIYLLFGVVLLVLGLWGLNAFATADPAKLLRFARQVALLAGATV
                     AVLLLAILLASRRFGFALLEVAAIALLVRWAWPRWRRGWAWPGSPATPVETPYIRMHF
                     DHASGTMSGMVQRGKFQGRDLAKLSRDQLLALWRECCTEDEPGAAMLEAYLDREIPDW
                     CQTRPDDAGQAIARAGDAMTTEEALAVLGLSAGAGKAEIRAAHRRLMAKLGPENGDSA
                     HLAAKLNQAQEILLRG"
     gene            2726..3049
                     /locus_tag="VGP50_04245"
     CDS             2726..3049
                     /locus_tag="VGP50_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002381365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEV8026613.1"
                     /translation="MLIATTERVAGHEVAETLGEVFGIAVRSRGLVGNVLAGLKTIVG
                     GEITQYTSMLQQARQHAIERMTAEAQLRGADAIVMMRFDSGEIAQGITEIVAYGTAVK
                     LRRVG"
     gene            3069..3683
                     /locus_tag="VGP50_04250"
     CDS             3069..3683
                     /locus_tag="VGP50_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEV8026614.1"
                     /translation="MERNVDYYFTPISPWTYLGHERLGAIAARHGAAIVCKPADYGKI
                     FSASGGLPLAKRPPQRQAYRLAELERWRRFLDIPLNLHPAFFPAPAELASRLILAAPA
                     AAQFRLTGALLRAVWAEERNIADEATLGRICSENALDGALLLETAKSPAVAERFDALT
                     QEAILRQVFGAPTYIHRDEPFWGQDRLDFLERALAASAKERKNK"
     gene            3829..>4531
                     /locus_tag="VGP50_04255"
     CDS             3829..>4531
                     /locus_tag="VGP50_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV8026615.1"
                     /translation="MAVTTERDAGAAPVLAAPRPPQQPLPLWRYLKVLRESVIATYGA
                     QAYSADIVERRFLWRRNLLVNEPNAIKHILVDNASSYGKTEVARRLLEPGLGRGLLTS
                     EGETWRRHRRIMAPAFDFRSIVGYAPIMTEIAGKLLAEWDALPDAAELDVAAVMMHAT
                     LHIISHTMFSSDSDGIVDLVERSVGRYQTEIRPGLIDILELPAWLSRLTFAPRGDAML
                     HEFDKVIDRLITSRER"
BASE COUNT          723 a         1573 c         1502 g          733 t
ORIGIN      
        1 gccgtcaccg tcgaaggcaa tgccgagatc ggcgccatcc gcgcgcaccg cctcctgcag
       61 ttgcaccaga ttcttcgcct cggtcggatc gggatgatgc gccggaaagc ggccgtcgat
      121 cgtctcgttg agcagccggt gacggccggg aaggcgctgc gtcaggcgtt gcaccgcttc
      181 gcccgtggcg ccattgccgc aatcccatac cacctgcagc ggccgcgcgc cttcgtaatc
      241 ggcgaggacc cgcgccacat aggcgtcgag caccagcttt tcctctacgc ggccggcgcg
      301 tgtgggcgtg gccgaggggg ccgcggcggc gatgcggccc aggccctgga tgtcggcgcc
      361 gaagaacgac ttctttccga gcatcagctt gaagccgttg tagtcggcgg ggttgtgcga
      421 ccccgtcacc atgatgccgc catcgacgcg ctcggtgttg acggcgaaat agagcatcgg
      481 cgtcggcccg aggccgatgc gcaacacgtc gatgccgctc ttggtgaggc cctcgatcag
      541 cgctgcctcg agcgtgggcg agctcagccg gccgtcgcgg ccgatggcca cgcgctttcc
      601 cccggcgtcc ctgaccaccg cggcgaaagc gcggccgatg gcccgcgcat cctcttcgcc
      661 cagcgttttg ccgacgatgc cgcggatgtc gtattcgcgc aggatcgtcg ggttgaagac
      721 gtggccggcg ccgtcgtgca tggttcgcct cagcaatact cgctcagaat atcgcgcagc
      781 gccggtcccg tgtctgggcg ggcgagcgcg aaggcgatgt tggcttgcag ccacccgacc
      841 ttgtcgccgc aatcgaaccg cgtgccctcg aagcgcaagc catggaacgg cttgttgccg
      901 cggatgagct cggccagcgc gtcggtgagc tgaatctcgt tgccggcgcc gcggccgacg
      961 cgctccagat gatcgaacac ctcgggctgc agaatgtagc ggcccacgat gctgagattg
     1021 gacggcgctt tcgccggctc gggcttctcc accacgccct tcatcttcac caacctgccc
     1081 tcctcgctct cgacgtcgag gatgccgtag cggttggtgt gcgcgtgcgg cacctccatg
     1141 atcgccacga cattgccgtc ggtcttggcc tgcgcctcga ccatctgcgc gaggcagggc
     1201 ttctccgcga ggatgagatc atcggccagc agcacggcga aaggctcctc gccgacgaga
     1261 ttgcgggcac accacaccgc gtggcccagg ccgagcggca cttgctggcg ggtataggcg
     1321 acctgccccg gctcgggcat ccaggagttg acggcgatga tctgcgcttc tttgccgcgc
     1381 tcgcgcagcg tgtcctcgag ctcgtaggag tgatcgaaat ggtcctcgat cgccgtcttc
     1441 gagcgtcccg tgacgaagat gaactcctcg atgccggcgg cccgcgcctc ttccacggca
     1501 tattggatca gcggcttgtc gaccaccggc agcatctcct tgggcatggc cttggtggct
     1561 ggaaggaagc gcgtgcccaa gcctccaacc ggaaatacgg ccttgcggat acgctgtgcc
     1621 atgagggtct ctttcactgg gggcgggctt ccgcaaataa cggagatcgc gtccggttgt
     1681 tcccttgcca cgcgaacggc gcggatgata tccgccgagt gctcgatcgg cgatttatag
     1741 gtccttgggt cgcgcagcgc caggagcctt caaccgcgga ggaggatttc ctgggcctga
     1801 ttaagcttcg ccgcgagatg ggccgagtcg ccgttctccg ggccgagctt cgccatgaga
     1861 cgacgatggg ccgcgcggat ttcggccttg ccggcgcccg ccgacaggcc aagcacagcg
     1921 agcgcttcct ccgtcgtcat cgcatcgccg gcgcgggcaa tcgcctggcc cgcgtcgtcg
     1981 gggcgggtct ggcaccagtc aggaatctcg cgatcgagat aggcttcgag catggctgcg
     2041 cccggctcgt cctcggtgca gcactcgcgc cacagcgcca gcagctggtc gcgcgacagc
     2101 ttggcgaggt cgcgcccttg gaacttgccg cgctggacca tccccgacat ggtcccgctc
     2161 gcatgatcga agtgcatgcg gatataaggt gtctcgaccg gtgtcgccgg gctgccgggc
     2221 cacgcccatc cccgccgcca ccggggccag gcccagcgga caagcagggc gatcgccgcc
     2281 acttcgagca aagcgaaacc gaagcggcgg ctggccagaa ggatcgccag cagcagcacc
     2341 gcaacggtgg cccccgccag cagcgcgacc tgtcgcgcga agcgcaggag cttcgccgga
     2401 tcggccgtgg cgaaggcgtt gagcccccag agccccagca ccaacaggac gacgccaaag
     2461 aggagataga tcacgggcgc ggcccaccca ggaggcttaa gattaagaat gcgcgtcagc
     2521 cccctcctcc ggagggagag gaaccgtgcg gcccggccgg catccgctcg acggaacgcc
     2581 ggccatgccg gtcgattatg cggtggccgg cgcgatcaat tccagcgagc aatttccggc
     2641 cgtgactcgg tgcccgggct cgcttaggct tcgccgcgaa gcagcccgcg gaaggcgccc
     2701 atcagcgaaa ctgcggagga aaatcatgct cattgcgacg acggagcgtg tcgccggtca
     2761 cgaggttgcc gagacgctcg gcgaggtctt cggcatcgcc gtccgcagcc gcggcctcgt
     2821 cggcaacgtc ctcgccggcc tcaagaccat cgtcggcggc gagatcacgc aatatacctc
     2881 gatgctgcag caggcgcgcc agcacgcgat cgagcgcatg accgccgaag cgcagctgcg
     2941 cggcgccgac gcgatcgtca tgatgcgttt cgattcgggc gagatcgccc agggcatcac
     3001 tgagatcgtc gcctatggca cggccgtgaa gctgcggcgc gtcggctaga tcctgatcgg
     3061 ggatgagcat ggaacggaac gtcgactact acttcacgcc gatctcgccc tggacctatc
     3121 tcggtcacga gcggctcggc gcgatcgccg cccgccacgg cgccgccatc gtctgcaagc
     3181 ccgccgatta cggcaagatc ttcagcgcct cgggtggcct tccgctcgcc aagcggccgc
     3241 cgcagcgtca ggcctaccgg ctcgccgagc tcgagcgctg gcgccgcttc ctcgacattc
     3301 cgctcaatct gcatcccgcc ttctttccgg caccggcgga gctcgcgtcg cgcctgatcc
     3361 tggcggcgcc ggcggccgcc cagttccgcc tgacgggcgc gctgctgcgc gccgtctggg
     3421 cggaggagcg caacatcgcc gacgaggcga cgctcggccg catctgcagc gagaacgccc
     3481 tcgacggcgc gctgcttctc gagacggcca aatcgcccgc tgtcgccgag cgcttcgacg
     3541 cgctgaccca ggaagcgatt ctgcgccagg tcttcggcgc gccgacctac atccatcggg
     3601 acgagccctt ctggggccag gaccgtctcg actttctcga acgcgcgctg gccgcctcgg
     3661 caaaggagcg caagaacaag tgacggcggt cgatcgccat gtcgaagcga cgcggcgctc
     3721 atcaccttgc gcccgcgtca cgggatgagg atgatcctgg agcggcgctg accgcgcgcg
     3781 ccgcggctct cgggccctga gggcgggctt caagggggat gaggacagat ggccgtcacc
     3841 acggagcgcg acgcgggagc agctccggtc ctcgcggcgc cgcggccgcc gcagcagcct
     3901 ttgcccctct ggcgctatct caaggtgctg cgcgagagtg tgatcgcgac ctatggcgcc
     3961 caggcctaca gcgccgatat cgtcgagcgc cggtttctct ggcggcgcaa cctcctcgtc
     4021 aacgagccca acgccatcaa gcacattctg gtcgacaatg cctcgagcta cggcaagacg
     4081 gaggtggcgc ggcggctgct cgagccgggc ctgggacgcg gcctcctgac gagcgaaggc
     4141 gagacatggc ggcgacaccg ccgcatcatg gcgccggcct tcgatttccg cagcatcgtc
     4201 ggctatgcgc cgatcatgac ggagatcgcc gggaagctgc tggccgaatg ggacgcgctt
     4261 cccgacgcgg ccgagctcga cgtggcggcg gtgatgatgc atgcgacctt gcacatcatc
     4321 tcgcacacca tgttttcctc cgattcggac ggcatcgtcg acctcgtcga gcgcagcgtg
     4381 ggccggtacc agaccgagat ccgcccaggg ctcatcgaca tcctggagct gccggcctgg
     4441 ctctcgcgcc tcaccttcgc gccgcgcggc gacgccatgc tgcatgagtt cgacaaggtc
     4501 atcgatcgcc tcatcacctc gcgcgagcgc g
//
LOCUS       DASYNS010000136         4519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3306081, whole genome shotgun sequence.
ACCESSION   DASYNS010000136 DASYNS010000000
VERSION     DASYNS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3306081"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VGP50_04260"
     CDS             complement(<1..405)
                     /locus_tag="VGP50_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026616.1"
                     /translation="MVSAVERRFEQGVARLVVLRPGEARAVAWSFGYFFTLLAAYYVL
                     RPLRDQAGITGGVKNLSWLFTATFLTMLVAQPIYGALVARLSRARFIPIVYHFFVANL
                     ALFWLLLTLGVAPVVMAWIFFVWISVFNLFAVS"
     gene            complement(491..1204)
                     /locus_tag="VGP50_04265"
     CDS             complement(491..1204)
                     /locus_tag="VGP50_04265"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEV8026617.1"
                     /translation="MSTPEPEPLRVEIAPEESVTALIYPASSVDRAGVTLILGHGAGA
                     DQTSAFMVSVATALAARGHDVVTFNFSYTERRGRLPDPNPRLEACYRSVIEAMRRHPP
                     LERNALAIGGKSMGGRIASQVAAAGVGALAGLVLLGYPLHPPGKPDRLRSKHLPAIAA
                     PLLFVQGEQDSFGTPDELRPILRSLTVPVELYVVAGGDHSFKVPKRAGVAQSDVDAAI
                     QERIDRWLRSNLIASGGGR"
     gene            complement(1292..2005)
                     /locus_tag="VGP50_04270"
     CDS             complement(1292..2005)
                     /locus_tag="VGP50_04270"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEV8026618.1"
                     /translation="MRYELYYWPSIQGRGEFVRLALEEAGADYVDVARRKGGMAKMMR
                     LLEGHRGRRLPFAPPFLKSGPLVIGQSALILMFLGERHGLAPANAAGRLWAHELQLTI
                     ADLVHEAHDTHHPIASSLYYEDQRPEARRRAADFLKSRAPKYLGYFEGVLARNPRYLV
                     GARLSYPDLSLFQVVEGLRYAFPRAMRRLERKYARVVELHDKVAARPRIAAYLASERR
                     IPFNEQGIFRHYPELDAQG"
     gene            complement(2155..2991)
                     /locus_tag="VGP50_04275"
     CDS             complement(2155..2991)
                     /locus_tag="VGP50_04275"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV8026619.1"
                     /translation="MSALQAIVIAVLQGVTELFPVSSLGHAVILPKLLGWSIDQKSLA
                     FLPFLVVLHLGTAAALLAYFWRDWLALALAFFGQGPAAERKAQWRLLWLIVVATIPAV
                     ILGAAFESFLRNLFGSPIAAAIFLVVNGGLLFVGERLRRESGTRRLAEMTWQEAIIIG
                     LWQSTALLPGISRSGATMVGGLLIGFHHREAAHFSFLIATPIILAAGVYQVPHLFHAG
                     AASFGPIVWIAGLVAGLTAYVSTVFLMRYFRRHDFEALDPFAYYCWAAGLIALGLFLF
                     VG"
     gene            complement(3008..3496)
                     /locus_tag="VGP50_04280"
     CDS             complement(3008..3496)
                     /locus_tag="VGP50_04280"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEV8026620.1"
                     /translation="MLKRVLLVVALLAVAPWAGAMATDEEKHQNIAKLVEMTGTLQNA
                     TALLDRMLPQMIDVIRKSNPEIPPQVLDALEKDGKDEFHKALPELIEPIIAIYDANYS
                     AEEVRQLLDFYQSPLGRKMIERMPQITQQSMAVGSAWGQSAGARVAERIRVSAKQKGY
                     DL"
     gene            complement(3614..>4519)
                     /locus_tag="VGP50_04285"
     CDS             complement(3614..>4519)
                     /locus_tag="VGP50_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV8026621.1"
                     /translation="VTHHKGATVWRLALTAPDASAADLASAVLATACSAVSAFEAGPQ
                     GVWRIEGYATTPPTPGLIEATLAVAWTARDDAPPALGVEPLPAHDWVALNQASFPPLA
                     AGRYVIHGSHHRERVPPGRIGLCIDAATAFGTGEHATTRGCLLAIDRLARRRHRPRTL
                     DMGTGTGILAIAAAKTWRRRVRARDIDAEAVRVAAHNAGINGVAGLLDLRRGNGYGDR
                     FLRRAVPFDLILANILARPLMRMAPALARALAPGGVAVLSGLLARQEAAVRAAHRQQG
                     LALVRRIALDGWHTLVLAKRPLRRG"
BASE COUNT          808 a         1482 c         1563 g          666 t
ORIGIN      
        1 cgacaccgcg aagagattga agacgctgat ccagacgaag aagatccagg ccatgacgac
       61 gggcgcgacg ccgagcgtga gcagcagcca gaacagggcg agattggcga cgaagaagtg
      121 atagacgatg ggaatgaagc gcgcgcggga gaggcgcgcc accagcgcgc catagatcgg
      181 ctgcgccacc agcatggtga ggaaggtggc ggtgaagagc cagcttagat tcttgactcc
      241 gccggtgatg ccggcctggt cgcgcaaggg acggagcacg taataggcgg caagcaacgt
      301 gaagaaatag ccgaacgacc acgcgacggc gcgtgcctcg cccggccgca gcaccaccaa
      361 ccgcgccacg ccttgctcga atcgccgctc taccgcgctc accatccatc acctctcagt
      421 cgtcctgata cgtttcggcg ttcagcccgt atcccctccc cgtcgctgaa cgcttcccgt
      481 cggcctttcg tcatctcccg cctccgctcg cgatgagatt gctgcgcagc cagcgatcga
      541 tccgctcctg gatcgccgcg tcgacgtcgc tctgcgccac gccggcacgc ttcggcacct
      601 tgaaggaatg gtcgccgcct gcgacgacat aaagctcgac gggaaccgtc aagctgcgca
      661 ggatcggccg cagctcgtcg ggcgtcccga agctgtcctg ctcgccctga acgaagagca
      721 gcggcgccgc gatcgccggc agatgcttgc tgcggagacg gtcgggtttg cccggcggat
      781 gcagggggta gccgagcagc accaggcccg cgagcgcgcc gacgcctgcg gccgcgacct
      841 gcgaggcgat gcgcccgccc atcgatttgc cgccgatcgc gagcgcgttg cgctcgagcg
      901 gcggatgacg ccgcatggcc tcgatgacgc tgcgatagca ggcctcgaga cgggggtttg
      961 gatcgggcag gcggccccgc cgctccgtgt aggaaaaatt gaaggtgacg acatcgtgac
     1021 cgcgtgcggc cagcgccgtc gcgacgctca ccatgaaggc gctcgtctga tcggcgccgg
     1081 cgccatggcc aaggatgaga gtgaccccgg cgcggtcgac ggaagaagcc ggatagatca
     1141 gcgccgtcac gctttcctcg ggcgcgatct cgacgcgaag cggctcaggt tccggtgtgg
     1201 acatctgtgt gtaacaacgg cacgtcaatt ttcccctctc ccgcgaagag cgggagaggg
     1261 gcagaaaatg caaatgtcca cgcaagctcg tctaaccctg cgcgtcgagc tcgggatagt
     1321 gacggaagat gccttgctcg ttgaagggaa tgcggcgctc ggaggcgaga taggcggcga
     1381 tgcgcggccg ggcggcgacc ttgtcgtgga gctcgacgac acgcgcatat ttgcgctcga
     1441 gccgtcgcat ggcgcgcggg aaggcgtagc gcaggccttc gaccacctgg aacagcgaga
     1501 gatcggggta gctcaaacgc gcgccgacga ggtagcgcgg attacgcgcg agcacgccct
     1561 cgaaatatcc gagatatttg ggcgcgcgtg atttcaggaa atcggcggca cgccggcgcg
     1621 cttccggccg ctgatcctcg tagtagaggc tgctggcgat cggatgatgc gtgtcgtgcg
     1681 cctcgtgcac gaggtcggcg atggtgagct gcagctcatg cgcccagagc cgcccggcgg
     1741 cgttggccgg cgcgagcccg tggcgctcgc cgaggaacat gaggatcaac gcgctctggc
     1801 cgatgaccag cggaccggat ttgagaaaag gcggggcaaa gggcagccgc ctgccccggt
     1861 gaccctcgag gagacgcatc atcttcgcca tgccgccttt gcggcgcgcg acatcgacat
     1921 agtcggcccc ggcctcttca agcgccaacc gcacgaactc gccgcgcccc tgaatcgacg
     1981 gccagtaata gagctcgtag cgcatcttcc gcccctcagg cgattgcgat ttaaaccctt
     2041 ttacttcgtc atccccgcga aagcggggac ccaggggcta ccggcacgtc gctcgccctg
     2101 gatccccgct ttcgcgggga cgacgtgaga aattgcttct accagcgaca tgctctaacc
     2161 gacgaacagg aagaggccca gcgcgatcag gcccgcggcc cagcagtaat aggcgaaggg
     2221 gtcgagggct tcgaagtcgt ggcggcggaa atagcgcatg aggaagacgg tgctgacata
     2281 ggccgtgagt ccggccacga gaccggcgat ccagacgatc ggaccgaagc tcgcggcccc
     2341 cgcgtggaag aggtgcggca cctgataaac gccggcggcg aggatgatcg gcgtggcgat
     2401 gaggaaggag aaatgcgcgg cttcgcggtg atggaagccg atcagcaggc cgccgaccat
     2461 cgtcgcgccc gagcgcgaga tccccggcaa cagcgccgtg ctctgccata gcccgatgat
     2521 gatcgcctcc tgccaggtca tctcggcgag gcgccgcgtg ccgctctccc gccggagccg
     2581 ctcgccgacg aagagcagcc cgccattgac gacgaggaaa atcgccgccg cgatcggcga
     2641 gccgaagaga ttgcggagaa agctctcgaa ggcggcgccg aggatcaccg ccggtatggt
     2701 ggcgacgacg atgagccaga gcaggcgcca ctgcgccttg cgctcggcgg cggggccctg
     2761 gccgaaaaac gccagcgcca gcgcgagcca gtcgcgccag aaataggcca gcaacgccgc
     2821 cgccgtgccg agatggagga cgacgaggaa cggcaggaag gcgaggctct tctggtcgat
     2881 gctccagccg agcagtttcg gcaggatcac ggcatgcccg aggctgctca cggggaagag
     2941 ctcggttaca ccctggagca cggcgatgac gatcgcctgg agcgcgctca tgacggcacc
     3001 gcatcgatca tagatcgtag cccttctgct tggcgctgac gcgaatccgc tcggcgaccc
     3061 gcgctccggc gctttgtccc caggcgcttc ccacggccat gctctgctgc gtgatctgcg
     3121 gcatccgctc gatcatcttc cggcccaggg gactctgata gaagtccagc agctgccgga
     3181 cctcctccgc cgaatagttg gcgtcgtaga tggcgatgat gggctcgatc agctcgggga
     3241 gggccttatg gaattcgtcc ttgccgtcct tctcgagagc atcgaggacc tgcggcggta
     3301 tctccggatt ggacttccgg atcacgtcga tcatctgcgg aagcatgcgg tcgagcaggg
     3361 ccgtggcgtt ttgcagcgtg ccggtcattt ccacgagttt cgcgatgttc tgatgctttt
     3421 cctcatccgt cgccatggcg ccggcccagg gcgcgacggc cagcagggcg acgacgagaa
     3481 ggacgcgctt cagcatgttc tgctcccctt tccgacgacg cggacatgac acaaggaggc
     3541 tcgggaacgc cgtccgccgc gtgttctaca ggccgcgcga ccggctgcaa agcgcgcctc
     3601 tccgactcgt tacctatcct cggcgcagcg ggcgctttgc caggacgagc gtgtgccagc
     3661 cgtccaaggc gatgcggcgg accagcgcca gcccctgctg gcgatgcgcc gcgcggaccg
     3721 cggcctcctg ccgcgccagc aatcccgaga gcacggcgac gccgccgggc gccagagcgc
     3781 gggccagcgc cggcgccatg cgcatgagcg gacgcgccag gatgttggcc aggatcagat
     3841 cgaacggcac ggcgcgacgc aagaagcggt cgccatagcc attgccccgg cgcagatcga
     3901 gaagccccgc gacgccgttg atccccgcat tatgcgcggc gacgcgcacg gcctcggcgt
     3961 cgatgtcgcg cgcccggacg cgtcgccgcc atgtcttggc ggcggcgatg gccaggatgc
     4021 ccgtgccggt gcccatatcg agcgtgcgcg gtcgatggcg acgcctggca aggcgatcga
     4081 tcgccaggag gcagccgcgc gtcgtcgcat gctcgccggt gccgaaggcc gtcgcggcat
     4141 cgatgcagag gccgatgcgg ccgggcggca cgcgctcgcg gtgatgcgag ccgtgaatca
     4201 catagcgccc ggcggcaagc ggcggaaagc tcgcctggtt gagcgcgacc cagtcatgcg
     4261 ccggcaaagg ctcgacgccg agggcgggcg gcgcatcgtc gcgcgcggtc caggcaaccg
     4321 ccagcgtcgc ctcgatcagg ccgggcgtgg gtggggtggt tgcatagcct tcgatgcgcc
     4381 agaccccctg gggccccgcc tcgaaagcgc tcaccgcgct gcaagcggtg gcgagcacgg
     4441 ccgaggcgag gtcggcggcg gacgcgtcgg gcgccgtcag cgcgaggcgc cagacggttg
     4501 cgcctttgtg gtgtgtcac
//
LOCUS       DASYNS010000137         5805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_809939, whole genome shotgun sequence.
ACCESSION   DASYNS010000137 DASYNS010000000
VERSION     DASYNS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5805
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809939"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VGP50_04290"
     CDS             <1..132
                     /locus_tag="VGP50_04290"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV8026622.1"
                     /translation="LKNGVSREEIAEVLLQAAIYCGVPAAVDAFRTAREAFKEVDGG"
     gene            196..272
                     /locus_tag="VGP50_04295"
     tRNA            196..272
                     /locus_tag="VGP50_04295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:230..232,aa:Arg,seq:cct)
     gene            523..999
                     /locus_tag="VGP50_04300"
     CDS             523..999
                     /locus_tag="VGP50_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026623.1"
                     /translation="MSLARRCLPLLFALALVGPVSALAQTSDAPQLRRDWADRYIVRL
                     DPDRKGYATLDDALRYAGAQFDRFDGGHAGVLDMASFTAAQRASLERASEARKPYAQR
                     ALDRREALFRTLDAKGEGKITKEAYLAMVRQHFADIDVKQAGKITAADLRLAHHGV"
     gene            1273..1884
                     /locus_tag="VGP50_04305"
     CDS             1273..1884
                     /locus_tag="VGP50_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEV8026624.1"
                     /translation="MTIKLHYHPFSTYSRRVRMACREKQIAYEPVLVDMTARKHREQP
                     YLSLNPYGRVPTLEEDGFVLYESTAILNYLEATHPKPALVPADPRQRALVDMHMKLCD
                     LQFGRPAVTIIFPKRFLPKERWNAAAIAEATKEIEKHLAVLDKQLTGKSFLVAEQFTL
                     AEICYAPFLEFLTLMEISPSPAVAAWSERILSRPSAKETRPER"
     gene            2166..3062
                     /locus_tag="VGP50_04310"
     CDS             2166..3062
                     /locus_tag="VGP50_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026625.1"
                     /translation="MGKTYGKNHVIIGSALSALLLTGSAWAQTTSFTATYQGTNSTTC
                     NTSFSIDGEEPTAAGAYPVFLYMVGTTETFSNASATAAVQGMANLGYVAGTIQYDSGS
                     FGSCSQIGAKAACIFNPNSSTSAVEVLCSRAKADCSKGIVVAGFSQGAVIATLAKNTD
                     SRVQAAYGMGDGVSYTQIFNLSSCMANGNRTLPSDRLRAVNGDKDQFTGSSESAQQTN
                     MQSLTGFTCASGSTSCLQSNGSGWIIVQDAQTEADDADHCYMRDGGCNASQNSLDTGW
                     KSGTANWELNANLNWLQGFTSH"
     gene            complement(3129..3842)
                     /locus_tag="VGP50_04315"
     CDS             complement(3129..3842)
                     /locus_tag="VGP50_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV8026626.1"
                     /translation="MSRTSESLIEDGHRLLMECPLFKPLEEDVRYQLAARARRGRFRA
                     GEVIFHMGSDGQSMMAVLTGRVRISIPSPQGKQVVLADLPAGEIFGEIALLDGRGRSA
                     DATALTNCDLVVLNRRDVLPILKQHPDVCLTLLEVVCRRLRDADERMTDVLFFNAPVR
                     LAKIILRGALARCEAGVGDAGLKVALSQGELGNMAGVRRERVNYCLREWRRRGIIELK
                     DGWIVVLKPSMLEELVQRG"
     gene            complement(3826..>5805)
                     /locus_tag="VGP50_04320"
     CDS             complement(3826..>5805)
                     /locus_tag="VGP50_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026627.1"
                     /translation="IAQERFGAVDLPDALARLVAERAGGNPLFAEEIASFLLERRIVR
                     RAGEGFEYDNDAVARALPASVQSLLSARIDRLEQSDRAILQAASVIGRRFDPDILAAA
                     SDDGDALRARLAALQTADLLQPGDASGEFVFKHALVRDALYGSLLTARRAALHQRIAA
                     AIERRSGNRPIEVAELLAHHYGMTDCVEKAFEYLALAGAKSIGVYSLDEADRYFARAM
                     EVATAFPGRVDPAKFGDLLVRYLQMLLMTSRFLKMSALAETHARTLETLSDQLPLALV
                     LAFHATSLMMQARFGEAASLSARAVRVASTAELRLILEEGASDDRAVGYVLTSAAAVL
                     TIAAACRPGDTEAMGLEAVRRSSGADDAFLPVWSLAALGWHYFCRGLLVEGRDCYRRL
                     LEVGQAQQDPRASSFGLWMLGYLAVFEEQYEEALDCGRRGEALALTPWDRTSSLSLQG
                     VALTLVGRVEEGLEKLTTATQMCREQGNQYTLSAQEPVFGIAMARQGRFRAGVAYIED
                     CVRRRDEEGDRVFADWTRLILAEMYLEFLAPRERAPLWVVLRNLPFLVHARLRGTGCA
                     LALLDRVLRNEQFSERGYICARAHLAIGLAHKIRNDGARAHEHLQRARGILAAIGTVQ
                     LLERVDALLSDLERRSSVYRTFRRRLVKIGHVANE"
BASE COUNT         1065 a         1991 c         1808 g          941 t
ORIGIN      
        1 ctcaagaacg gcgtctcgcg cgaggagatc gccgaagtgc tgctgcaggc ggcgatctat
       61 tgcggcgtgc cggcggccgt cgacgccttc cgcacggcgc gcgaggcgtt caaggaagtg
      121 gatggcggtt gagccgtgcg acgtagcgtg ccgccgagtg gcggcacgcc cgcgcgattg
      181 ctataaccgc gccacgtccc cgtagctcag ccggatagag caacagtttc ctaaactgtg
      241 ggtcgcgtgt tcgagtcacg ccggggacgc caccctgcgc cagagcacga cagggtgtcc
      301 tccggccgcc gatctccgct catatcaaac aacctcaccc tgtccgcatc gcgagacctg
      361 aatggcgtct cgcgcaattt tacctcatcg gaggtgaggt gcgttccgtg tcgttcgaga
      421 tcacgcaaac tttgtgcttg acgaccagaa caaatagtga acaatggcga cgtccaggaa
      481 ttttctagcc gattccgcta agcctaagaa ggaggtttct tcatgtcgct cgctcgccgt
      541 tgccttccgc ttctcttcgc tctcgccttg gtgggtccgg tcagcgcgct cgcccagacc
      601 agcgacgcgc cgcagctccg gcgcgactgg gccgatcgct acatcgtccg gctcgatccc
      661 gaccggaagg gctacgccac gctcgacgac gctctgcgct atgccggcgc ccagttcgac
      721 cgcttcgacg gcggccatgc gggcgtgctt gacatggcga gcttcaccgc cgcacagcgc
      781 gcctcgctcg agcgcgcgag cgaggcccgc aaaccctacg cccagcgcgc cctcgaccgc
      841 cgcgaggcgc tcttccgcac gctcgacgcg aagggcgaag gcaagatcac caaggaggcc
      901 tatctcgcga tggtacgcca gcatttcgcc gacattgacg tgaagcaggc cggcaagatc
      961 accgctgccg acctccgcct cgcgcatcac ggcgtctgat caggccgcgc ctcgccgccg
     1021 accggccgag cggcgaggcg cattgcccct ttcggtgttg ccggcgagcc agcctggccg
     1081 gatcctcatc aatcgtggca cgccaaccct gcgcccgtgc cgttccgctt cggcccgtcg
     1141 gcaacgaaaa cgccggtacg tatcggcggc cagcgcagag ggggcccgct tttgtgccgc
     1201 cagatcgcgc tgggcaagat caccgggcgg ttgcatgatc gcgcctgcgt gcgagatcgg
     1261 gagcgaggcg tcatgaccat caagctgcac taccatccct tttccaccta ttcgcgccgc
     1321 gtgcggatgg cgtgccgcga gaagcagatc gcctatgagc cggtgctggt cgacatgacg
     1381 gcgcgcaaac atcgcgagca gccttatctc tcgctcaatc cttatggccg cgtcccgacg
     1441 ctcgaagagg acggcttcgt tctctacgag tcgacggcga tcctcaacta cctcgaggcg
     1501 acgcatccga aaccggcgct ggtgcccgcc gatccgcggc agcgcgcgct ggtcgacatg
     1561 catatgaagc tctgcgatct gcaattcggc cgcccggccg tcacgatcat cttccccaag
     1621 cgcttcctgc cgaaggagcg ttggaacgcg gcagcgatcg cggaggcgac gaaggagatc
     1681 gaaaagcatc tcgccgttct cgacaagcaa ctgacgggta agagcttcct cgtggcggag
     1741 caattcacgc tcgccgagat ctgctacgcc cctttcctcg agttcctgac gctgatggag
     1801 atcagcccat cgcccgccgt ggcggcgtgg agcgagagaa tcttgtcgcg gcccagcgcc
     1861 aaggagacgc ggcccgaacg ctaggcgcgc gcagaacgcc tccgcgttct accgcgacgc
     1921 ttgcggcatt caccgaaact tgaataatac ggtaaccata gctgttgtca gattgtctga
     1981 caatctgtaa ggttgtagcg tctcggtctc cgctcttccg cagggaagag cgccggggca
     2041 catatttctt ttgtcgaatg cacgctgcgc gctcgcgcca atcagaaaag acccgcatca
     2101 cgcggggcag catcgaagca gacatcgagc ggggcgtcag tccccgcgca acggaggaac
     2161 gagacatggg aaagacgtac ggcaaaaatc acgtaatcat cgggtcggcg ctgtcggcgc
     2221 ttttgctgac aggctccgcc tgggcccaga cgaccagctt cacggcaacc tatcaaggca
     2281 ccaattccac cacctgcaac acgtccttca gcatcgatgg cgaggagccg acggcggcgg
     2341 gcgcctatcc ggtctttctc tacatggttg gtacgaccga gaccttcagc aacgcttcgg
     2401 cgaccgcggc cgtccagggc atggccaatc tcggctacgt cgccggcacc atccagtacg
     2461 acagcggcag tttcggcagc tgctcgcaga tcggcgcgaa agcggcgtgc atcttcaatc
     2521 cgaatagctc gaccagcgcc gtcgaagtgc tgtgctcgag ggccaaggcc gactgtagca
     2581 agggcatcgt cgtcgccggc ttcagccagg gcgcggtcat cgccaccttg gcgaagaata
     2641 ccgactcccg ggtccaggcc gcttacggca tgggcgacgg cgtcagctat acgcagatct
     2701 tcaacctcag ctcctgcatg gccaacggca atcgcacgct gccgagcgac cgcctccgcg
     2761 ccgtcaacgg cgacaaagac cagttcaccg ggagcagcga gtcggcgcag cagaccaaca
     2821 tgcagagcct gacgggcttt acctgtgctt cgggctcgac gagctgcctg cagagcaacg
     2881 gcagcggctg gatcatcgtc caggacgcgc agaccgaggc cgacgacgcc gaccactgtt
     2941 acatgcgcga cggcgggtgc aacgccagcc agaacagcct ggacaccggc tggaagagcg
     3001 gcacggcgaa ttgggagctc aacgccaatc tgaactggct ccagggcttc acctcgcact
     3061 gaggttccac ggacatctgt gggaaacggg gccgcgaggc cccgtttttc attgccgaac
     3121 agccatagtc agcctcgttg gaccagttcc tccaacatcg acggtttcaa aacgacgatc
     3181 cagccgtcct tgagctcgat gatgccgcgc cgccgccact cgcgcaggca gtaattgacc
     3241 ctctcccgac ggacacccgc catgtttccc aactcgcctt gggacagggc gactttcaga
     3301 ccggcatcgc cgacgcccgc ttcgcaacgg gcaagagcgc cgcgcaggat gattttcgcc
     3361 agtcgcaccg gcgcattgaa gaacaagacg tcggtcatgc gctcgtcggc gtcgcgcagc
     3421 cggcggcata cgacctcgag cagtgtgaga cacacatcag gatgctgttt gaggatcggc
     3481 aggacgtcgc gccgattcaa cacgacgagg tcgcaattgg tcagcgcggt ggcgtccgcc
     3541 gagcggccgc ggccatcgag gagagcgatt tcgccgaaga tctcgccggc cggcagatcg
     3601 gcgagaacga cctgcttgcc ctggggagac gggatggaaa tgcgcaccct gcccgtcagc
     3661 acggccatca tgctctgccc gtccgacccc atgtgaaaaa tcacctcgcc ggcgcggaag
     3721 cggccgcgac gcgcgcgcgc cgccagctga tatcgcacat cctcttcgag cggcttgaag
     3781 agcgggcatt ccatcaggag ccgatgcccg tcttcgatca aggattcact cgttcgcgac
     3841 atggcctatc tttacaaggc gtcgtcgaaa cgtccggtag acgctgctcc ggcgctcgag
     3901 atcggagagc agcgcatcga ccctttccag cagctggacc gtcccgattg ccgccaagat
     3961 cccgcgggcg cgttgcaaat gctcgtgggc gcgtgccccg tcgttccgga tcttgtgggc
     4021 gaggccgatc gcgagatgag cgcgcgcgca aatatatcca cgctcgctga attgctcgtt
     4081 gcggagcacc cggtcgagca gggcgagggc gcaaccggtg ccccggagcc gggcgtggac
     4141 gaggaacggc aggttgcgca gcacgaccca gagcggcgcc cgctctcgcg gcgccaggaa
     4201 ctccaggtac atctcggcaa ggatcagtcg cgtccagtcg gcgaacacac ggtcgccctc
     4261 ctcgtcccgg cggcggacgc agtcctcgat ataggcgaca cccgcgcgaa aacgtccctg
     4321 ccgcgccatt gcgatgccga agaccggctc ttgcgccgac aacgtatatt ggttgccttg
     4381 ctcgcggcac atctgcgtcg cggtggtgag tttctccagc ccctcctcga cccttcccac
     4441 cagcgtcagc gccacgccct ggagcgagag cgaggacgtc ctgtcccagg gcgtcagcgc
     4501 cagcgcttcc ccgcgacggc cgcagtcgag agcctcttca tattgctcct cgaacaccgc
     4561 cagatatccc agcatccaga gtccgaagga gctcgcccgc ggatcctgtt gcgcctgccc
     4621 cacctcaagg aggcggcgat agcaatcccg tccctcgacc agcagcccgc ggcagaagta
     4681 gtgccatccc aatgcagcga gcgaccacac cggcaggaag gcatcgtcgg cgccgctgct
     4741 ccgccgcacg gcttcgaggc ccatcgcttc cgtgtcgccg ggccggcacg cggcggcgat
     4801 ggtcaggact gccgcagcgc tcgtcaatac gtagccgacg gcgcggtcgt cgctcgcccc
     4861 ctcctcgagg atcaagcgca attccgcggt tgaggcgacc cgtaccgctc tcgccgagag
     4921 agaggccgct tcgccgaacc gcgcctgcat catcagggag gtcgcgtgga atgccaggac
     4981 cagggcgagc ggcagctggt ccgagagagt ctcgagcgtt cgcgcatgcg tctccgcgag
     5041 cgcgctcatc ttcaggaagc gcgacgtcat cagcagcatc tgcagatacc tgacgaggag
     5101 atcgccgaac ttcgccggat caacgcgtcc cgggaacgcg gtcgccacct ccatggcgcg
     5161 cgcgaaataa cggtccgcct catccagaga gtagacgccg atgctcttgg cgccggcaag
     5221 cgccaggtac tcgaaggctt tctcgacgca atcggtcatc ccatagtggt gcgcgagaag
     5281 ctccgcgact tcgatcggcc ggttgccgct ccgccgctcg atcgccgcgg cgattcgctg
     5341 gtgaagggcc gcgcggcggg ccgtcaacag gctgccatag agcgcatcgc gcaccagcgc
     5401 gtgcttgaag acgaactcgc ccgaggcatc gccgggctgg agaagatccg ccgtctggag
     5461 agcggcgagc cgcgcgcgga gggcgtctcc gtcatcgctc gccgcggcga gaatatcggg
     5521 atcgaagcga cggccgatca ccgacgccgc ctgcagtatc gcccggtcgc tctgctcgag
     5581 gcggtcgatg cgcgcgctga ggagcgactg cacgctcgcc ggcaacgcgc gggccacggc
     5641 gtcgttgtcg tattcgaaac cttcccccgc ccgccgcacg atgcgtcgct cgagcaggaa
     5701 gctcgcgatc tcctccgcga aaagcggatt cccgccggcc ctttcggcca ccagccgcgc
     5761 cagcgcgtcc ggcaggtcga cggcgccaaa gcgctcctgc gcgat
//
LOCUS       DASYNS010000138         9376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2995349, whole genome shotgun sequence.
ACCESSION   DASYNS010000138 DASYNS010000000
VERSION     DASYNS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 9376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9376
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2995349"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VGP50_04325"
     CDS             complement(<1..306)
                     /locus_tag="VGP50_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV8026628.1"
                     /translation="MTVIEISEPGGPDVLRPATRPMPLPGAGEVLVQVTAAGVNRPDV
                     LQRQGGYPPPPGASDIPGLEIAGTVIAIGLSVRRAKVGDAVCALVSGGGYASHCVAPE
                     "
     gene            522..704
                     /locus_tag="VGP50_04330"
     CDS             522..704
                     /locus_tag="VGP50_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEV8026629.1"
                     /translation="MDEEDLLPRQKKPQKKDLTLLGIAELEAYIGELEEEIARVRTEM
                     AVKRKQRTGADALFKR"
     gene            706..1779
                     /locus_tag="VGP50_04335"
     CDS             706..1779
                     /locus_tag="VGP50_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026630.1"
                     /translation="MEHVAEIPLTVVGAGGAADLFAAHPEKAAALIATALKSHPLLPL
                     GAAVADVISRRWLERQQNPYLDEIHRVASGLARPGVYFLNIVYEWACSTSAGPDPRGC
                     GMRLIRVLDWGLHGIGRHVVIAQQPGRHGVFYNSTWPGFAGVLTAMAPGRFAAAINQA
                     PRLPIFGPRWIDEVAGRVRMLRSKGVIPAAHLLRRVFEEARDYGEARTMLADERQRLA
                     MPALFTLAGVGPREACVVEAIAQQRRVHDAPADDGCTIGVANDWLSGDLPGIPRAHSL
                     GPSPAVSPRENNRMRRSIVCVLQQGAFGGAADLVPPVLNSHTVMVVSANAAIGEMTVE
                     ALDPLAGAGPLPRVVARRALRHA"
     gene            1826..2611
                     /locus_tag="VGP50_04340"
     CDS             1826..2611
                     /locus_tag="VGP50_04340"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEV8026631.1"
                     /translation="MVLQGKAAVVTGSTSGIGLGIATALAAQGSAVMLNGFGDAGAIE
                     RLRRDLAERHGVKVGYSAADMTKPAEIRQMIADAARQLGSVDILVNNAGIQHVAPVED
                     FPDDKWDAIIAINLSAAFHATKAALPGMKQRRWGRIINIASAHGLVASPDKAAYVAAK
                     HGIVGLTKVVAIETANDGITCNAICPGWVLTPLVQKQIDARAKETGRTAEQEKIELVR
                     EKQPMLAYTKPEQIGALSVFLCGEASATMTGAALPIDGGWTAQ"
     gene            3110..4423
                     /locus_tag="VGP50_04345"
     CDS             3110..4423
                     /locus_tag="VGP50_04345"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEV8026632.1"
                     /translation="MPDPKFPKFATLSLHAGQRPDPTTGARAVPIYQTTSYVFRDSDH
                     AAALFNLEEPGYLYSRIANPTVAVLEERLAALEGGVGAVCTASGQAALHVAIATLMGA
                     GGHIVAAASLYGGSINLLKLTLPRFGITTTFVDPRDPAGFRRALRPETRLVFAEILGN
                     PGLEVLDVPRIAQVAHEAALPLMVDATFATPYLCRPIEHGCDIVLHSATKWLGGHGVA
                     IGGALIDGGRFDWAASGNFPTLSEPYAGYHGVDFAAEFGPLAFVMRARAEGLRDFGAC
                     MSPANAFHLLQGVETLPLRMARHIENTREILRFLVASDAVAWVTHPDLPAHPDHALAQ
                     RLMPLGAGSIVSFGIKGGRGAGRRFIDSLELVSHLANVGDAKTLVIHPASTTHQQLDA
                     AALAAAGVGEDMVRLSVGLEDPQDIVDDLAQALRASQKGLRAAAE"
     gene            4433..5221
                     /locus_tag="VGP50_04350"
     CDS             4433..5221
                     /locus_tag="VGP50_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV8026633.1"
                     /translation="MDLSVDGKRVVAATGGRAPDPATTTALLLHGAGMDHTAFALQAR
                     ALAHHQRNVLAFDLPGHGGSAGPALETIGALAGWVLRAAAAAGVERFRIAGHSMGALI
                     ALEVAAQANERVEALALLGFAPEMRVHPDLLAHARAGSHRAVELMTAWSLGAQGRLGG
                     NFSPGLWLTGGALRLLERADPASLGADLVACDAYRGAEAAAARVRCPALLLLGAEDRM
                     TPAARGQAFATRIAGSRVVVLPGVGHMMTMEDPGATIRVMRQIL"
     gene            complement(5286..5729)
                     /locus_tag="VGP50_04355"
     CDS             complement(5286..5729)
                     /locus_tag="VGP50_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEV8026634.1"
                     /translation="MDAFDLSPLFRSTIGFDRLARLTEAPRADAYPPYNIETTGENAY
                     RLTMAVAGFASTDLEITVKDNALLVSGKSKPEEDAKKYLHRGIARRAFERRFQLAEHI
                     KVAGASLDNGLLHVDLAREIPETMKPRKIEINGGNGPRVVDQKAA"
     gene            5902..6312
                     /locus_tag="VGP50_04360"
     CDS             5902..6312
                     /locus_tag="VGP50_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEV8026635.1"
                     /translation="MQQQARATEFFGRTYGEAMSLLVEARDYLAHREPIERHALLPPV
                     RLRMSCETMRLTARLTQIMGWLLSQRAVYEGELSAEAALDGQDVLGDIEVCMADDAAG
                     TTELPQRLRHLLERSRRLFIRVARLDELARRQID"
     gene            complement(6314..6538)
                     /gene="rpmE"
                     /locus_tag="VGP50_04365"
     CDS             complement(6314..6538)
                     /gene="rpmE"
                     /locus_tag="VGP50_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007281980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEV8026636.1"
                     /translation="MKDGIHPDYHEITVVMTDGTSYKTRSTYGKAGDTLRLEIDSKSH
                     PAWTGGQQRLLDGGQLARFNKRFQGIGLKK"
     gene            complement(6983..7990)
                     /locus_tag="VGP50_04370"
     CDS             complement(6983..7990)
                     /locus_tag="VGP50_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HEV8026637.1"
                     /translation="MPLASRRNRRSNIEVWPGFVDALSQLVIVIIFVLLIFTAGQFFL
                     SDALYGSNEALARLNSQISDLVDQLAFEKKTSEDLRHDLSQLTSQLQAANVERDALTA
                     RLAEMSARADDAAARANRSASDLAGAQSTVDQVNQQLAALREQLAQLAAALDASEAKS
                     KDQQVQIADLGKRLNAALASKVEELARYRSEFFGRLREVLGDRPDIRVVGDRFVFQSE
                     VLFGMASADLSDNAKRQLLPVAQALKQIAAKIPPDIEWVLRVDGHTDKRPIATPQFRS
                     NWELSSARAISVVRFFIDQGIPANHLVAAGFGEFQPIDARDTEDAYQRNRRIELKLTE
                     R"
     gene            complement(7994..9139)
                     /locus_tag="VGP50_04375"
     CDS             complement(7994..9139)
                     /locus_tag="VGP50_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HEV8026638.1"
                     /translation="MTRPSRYLVRMVVFMVAVLGLAAVLAQPLYRYFMGNWLVNGVIL
                     FILAIGTVYIFRQVILLYPEIGWIERFRRNQNNNVPSEDMPRLLSSLATMLADRRGGK
                     MTLSALSMRTLLDGIQTRLDETRESSDYLRGLLLFLGLLGTFYGLLQTVQSMPAIVQG
                     LSVGTTDAARAFSDFKAGLMPILSGMGTAFSASLFGLAGSLVIGFLGLQATLAQNLFY
                     NGLEEWLSGYTRLSGSALGGEGGDGSVPAFIQALLEQTADGLENLQRIMTRGEDSRIA
                     ANANTLALTERLTTLADQMRIGQNLLLKLAEQQGELKPVLTRLAEGAAGGGQDETRGH
                     LRNIEVYLARLAEDLPQGRGEAVQEIRSEIRLLARTIAALAQEPQRP"
     gene            complement(9142..>9376)
                     /locus_tag="VGP50_04380"
     CDS             complement(9142..>9376)
                     /locus_tag="VGP50_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV8026639.1"
                     /translation="EWLDTNQQARIEVVAYAAGSAEQANDARRTSLTRALAVRKYLAD
                     HGVATTRMEVRALGNHSAEGDPPDRVDVAPMEH"
BASE COUNT         1547 a         3069 c         3192 g         1568 t
ORIGIN      
        1 ctcgggcgca acgcagtggc tcgcatatcc gccgcccgaa acgagggcgc agacggcgtc
       61 accgactttg gcgcggcgga cgctgaggcc gatcgcgatc accgtaccgg cgatctcgag
      121 ccccggaatg tccgaggcgc ctggaggcgg cggatagccg ccctgacgct gaagaacgtc
      181 cggccggttg acacccgcag ccgtcacctg gaccagcact tcgccggcac caggaagagg
      241 catcggccgg gtcgccggtc ggagcacatc gggtccgccc ggctcgctga tctcgatcac
      301 ggtcatggtg gcgggaagcg tcgtcacaac ggaaatcctt tgccagaggt gatcgtgggt
      361 ttagccgatg agagcggtca tggcgactct tgtcggctcc gagcaagcag gttaacgatt
      421 cacaatgcct gctaaggcat gttccgcgct gcggtcgatc gattgcgcct ctcgcgtgat
      481 cgcgccaaag tggctcgttg tgggctcctg aggaggaacg gatggacgaa gaggacctgc
      541 tcccgcgtca gaagaagcca cagaagaagg atctgacgct gctcgggatt gccgagctcg
      601 aagcttatat cggcgagctc gaagaagaga tcgcccgcgt ccggaccgag atggcggtca
      661 agcgcaagca gcgcacgggc gccgacgcgc tgttcaagcg ctgagatgga acacgtcgcg
      721 gaaataccgc tcaccgtcgt gggtgccggc ggcgcggccg atctcttcgc ggcgcatccc
      781 gagaaggctg cggcgctgat cgcgaccgcg cttaagagcc atccgctgct ccccttgggg
      841 gcggcggtgg cggatgtcat ctcgcggcgc tggctcgagc gtcagcagaa cccctatctc
      901 gacgagatcc accgcgtggc ctcgggattg gcgcgtcccg gcgtctattt cctgaacatc
      961 gtctatgaat gggcctgctc gaccagcgct ggacccgatc cgcgcggctg cggcatgcgg
     1021 ctcatccgcg tgctcgactg ggggctccat gggatcggca ggcatgtcgt catcgcgcaa
     1081 cagccgggcc gccatggcgt cttctacaac agcacatggc cagggtttgc cggcgttctg
     1141 accgccatgg cgcccggccg cttcgccgcg gcgatcaatc aggcgccgcg cctgccgatc
     1201 ttcggaccgc gttggatcga cgaggtggcg ggccgcgtac gcatgctgcg cagcaagggg
     1261 gtgatcccgg cggcgcatct gctccggcgc gtcttcgagg aagcgcgcga ttacggcgaa
     1321 gcgcggacga tgctcgctga cgagcggcag cgcctggcca tgccggcgct cttcaccctc
     1381 gccggcgtcg ggccgcggga ggcttgtgtg gtggaggcga tcgcacagca gcggcgcgtc
     1441 catgacgcac ccgccgacga cggctgcacc atcggcgtcg ccaatgattg gctgagcggc
     1501 gatctgcctg gcataccgcg cgcccattcg ctcggtccgt cgcccgcggt gagtccgcgc
     1561 gagaacaacc gcatgcggcg gagcatcgtc tgcgtgctgc agcagggtgc cttcggcggt
     1621 gcagcggatc ttgtcccccc ggtgctcaac agccataccg tcatggtggt gagcgccaac
     1681 gccgcgatcg gagagatgac ggtcgaggcg ctcgatccgc tggccggcgc cggcccgctg
     1741 ccgcgcgtcg tcgcccggcg ggcgctccga cacgcgtgac gaaccttgaa ggcgccgatc
     1801 caaccgcgaa gaggagagaa ggaagatggt attgcagggc aaggccgcgg tggtcaccgg
     1861 ctcgaccagc ggcatcgggc tgggtatcgc taccgccctc gcggcgcagg gatcggccgt
     1921 catgctcaat ggcttcggcg atgccggggc gatcgagagg ctgaggcgcg atctcgccga
     1981 gcggcacggc gtcaaggtcg gctattccgc cgccgacatg acgaagccgg ccgagattcg
     2041 ccagatgatc gccgacgccg cccgccagct cggctcggtc gacatcctcg tcaacaatgc
     2101 cggcatccag catgtggctc cggttgagga tttccccgac gacaaatggg acgcgatcat
     2161 cgccatcaat ctctccgccg ccttccacgc gaccaaggcg gcgcttcccg ggatgaagca
     2221 gcggcgctgg ggccgcatca tcaatatcgc ctcggcccat gggctggtgg cgagccccga
     2281 caaggcggcg tatgtcgcgg ccaagcacgg catcgtcgga ctgaccaagg tcgtggcgat
     2341 cgagacggcc aatgacggca tcacctgcaa cgccatctgc cccggctggg tgctgacgcc
     2401 gttggtgcag aaacagatcg acgcgcgggc caaggagaca ggacggacgg cggagcagga
     2461 gaagatcgaa ctggtgcgcg aaaaacagcc gatgctcgcc tacaccaagc ccgagcagat
     2521 cggtgcactc tccgtctttc tctgcggcga ggcgagcgcg accatgacgg gcgctgcgct
     2581 gccgatcgac gggggctgga cggcgcagta gccgaaccgg gatctgccgc cggctcaacg
     2641 gcgcgtcgcg cgctcgccgg cgatccaggg cgggtcgaga ttccccctct gcccaggggt
     2701 gcgcgagggt cagctgcgtg atccgccagg cgccgtcgag cttctcgaag cgcgtctcat
     2761 aacacccgcc gacgagcaga ggtcgtttgc cggcgagcgc ccggtccttg atgacgtgcc
     2821 aggcgacgac caaggctcag ccccgaccgg attggtcacc tgacataatg gccgcgcgtc
     2881 ccggcgcggc aattaccatc gtaatgggct aaaatcgctt cagcatctct taacgatcgg
     2941 ccgcaatact gcccaggtgg accggggctc tgccccgccg catctcaggc atcatccgca
     3001 cattcaaacc ttgcaccttc aaggggccgt cactcgctga cggctccggc tattcccgga
     3061 tcggcgatgg tgtaaggtag gcgcgattgg tgtctgcccc ggtgctgcca tgccagatcc
     3121 aaaatttccg aaatttgcca cgctcagcct gcatgccggg cagcggcccg atcccacgac
     3181 cggcgcccgc gccgtgccga tctaccagac gacgagctac gtgttccgcg acagcgacca
     3241 tgccgcggcg ctgttcaatc tcgaagagcc tggctatctc tattcgcgca tcgccaatcc
     3301 caccgtggcg gtgctcgaag aacgcttggc cgcgctcgag ggcggcgtcg gcgccgtctg
     3361 cacggcgagc gggcaggcgg cactgcacgt cgcgatagcg actctgatgg gcgccggcgg
     3421 ccatatcgtc gcggccgcct cgctctatgg cgggtcgatc aacctcttga agctgacctt
     3481 gccgcgcttc ggcatcacga ccaccttcgt cgatccgcgc gatccggcgg gttttcgccg
     3541 ggcgcttcgt ccggagaccc gcctcgtctt cgccgagatc ctcggcaatc ccgggctcga
     3601 ggtgctcgac gtgccgcgga ttgcacaggt cgcgcatgag gcggcgctgc cgctcatggt
     3661 cgatgccact ttcgcaacgc cctatctctg tcggccgatc gagcatggct gcgacatcgt
     3721 gctgcactcg gcgaccaagt ggctcggcgg ccatggcgtc gccatcggtg gggcgcttat
     3781 cgatggcggc cgcttcgact gggcggcttc gggcaacttc ccgacgctga gcgagcccta
     3841 tgccggatat catggtgtcg acttcgccgc cgagttcggt cccctggcct tcgtcatgcg
     3901 cgctcgggcc gagggcctgc gcgatttcgg cgcctgcatg agcccggcca acgcctttca
     3961 tctgctgcag ggcgtcgaaa cgctgccgct acgcatggcg cggcatatcg agaatacgcg
     4021 cgagatcctt cgctttctcg tggcgtcgga tgccgtcgcc tgggtcacgc atcccgatct
     4081 gcccgcccat cccgaccatg cactcgccca gcgtctgatg cccttggggg ccgggtccat
     4141 cgtcagcttc gggatcaagg gcggccgcgg cgcgggacgg cgtttcatcg acagcctcga
     4201 actcgtgtcg catctcgcca atgtcggcga cgccaagact ttggtcattc atcccgcaag
     4261 cacgacacat cagcagctcg acgccgccgc gctcgctgcc gcgggggtcg gcgaggacat
     4321 ggtgcgcctc tcggtcgggc tcgaggatcc acaggacatc gtcgacgacc tggcgcaggc
     4381 cctgcgagcg tcgcagaagg gcctgcgggc ggcggcggaa tagagcgccg ccatggatct
     4441 gtcggtcgac ggcaagcgcg ttgtcgcggc gaccggcggg cgtgcgccag atccggcgac
     4501 aacgacggcg ctgctgctgc acggcgcggg aatggaccac accgctttcg cgcttcaagc
     4561 ccgcgcactc gctcatcacc agcgcaacgt cctcgctttc gatctgccgg ggcatggcgg
     4621 cagcgccggt ccggcgctcg aaaccatcgg cgcgctggcg ggctgggtgt tgcgcgcggc
     4681 ggcggccgcc ggggtcgagc gcttccgcat tgccgggcac agcatggggg cgctcatcgc
     4741 tctcgaggtt gccgcgcagg ccaacgaacg cgtcgaagcg ctggcgcttt tgggcttcgc
     4801 gccggagatg cgcgtgcatc ccgacctgct ggcgcacgcg cgcgccggca gtcatcgcgc
     4861 ggtcgagctg atgacggcct ggagcctcgg tgcacaaggg cggctcggcg gcaatttctc
     4921 gccggggcta tggctcaccg gcggcgcgct ccgtctcctc gagcgcgccg atccggcgag
     4981 cctcggcgcc gatctcgtgg cctgcgacgc ttatcgcggc gcggaagcag cggccgcgcg
     5041 ggtgcgctgc ccggcattgc tgctgctcgg tgccgaggac cgcatgacgc cggcggcgcg
     5101 cggccaagcg tttgcaacgc gcatcgcggg gagccgcgtc gtggtgctgc cgggcgtcgg
     5161 ccatatgatg accatggaag atcccggcgc gacgatccgg gtgatgcggc aaatcctttg
     5221 agtgcgtgac aaaaaagggg cagctctcgc gagctgcccc tttcgttcgt ccgaccgtga
     5281 ggcggttacg cggctttctg atcgaccacg cgcggaccat tgccgccgtt gatctcgatc
     5341 ttgcgcggct tcatcgtttc cgggatctcg cgcgccagat cgacatggag caggccgttg
     5401 tcgaggcttg caccggcgac cttgatatgc tcggcgagct ggaagcgccg ctcgaaggcg
     5461 cgtcgcgcga tgccgcgatg gaggtatttc ttcgcgtcct cctcgggctt cgacttgccg
     5521 ctgaccagga gagcgttgtc cttaaccgta atctcgagat ccgtcgacgc gaagccggcc
     5581 accgccatgg ttaagcgata ggcgttctcg ccggtggtct cgatgttgta gggcgggtag
     5641 gcgtccgcgc gaggagcttc ggtaagccgc gccaggcggt cgaaaccgat ggtcgagcgg
     5701 aaaagcggag agagatcgaa agcgtccatg accacatcct ccttaatgag caacatgggg
     5761 tctcgcttgg cccgccatat ggccggtgcc agtgcaaggc cggaccctca aagcggcgtc
     5821 cgactaacgc ggaaataagc attcatggtt atcattcaag ggggtatcgc caaccggcgc
     5881 caatggtgat agggaatcct ggtgcagcag caagcgcgag cgacggaatt cttcggccgt
     5941 acctatggcg aggcgatgtc gcttctcgtc gaggcccgcg attatctcgc ccatcgcgag
     6001 ccgatcgagc gtcacgcgct gctaccgccc gttcgcctgc gcatgtcgtg cgagaccatg
     6061 cggctcaccg cgcggctcac gcaaatcatg gggtggctct tgtcgcagcg cgcggtctat
     6121 gagggcgagc tctcggcgga agcggcgctc gacgggcagg atgtgctcgg agatatcgag
     6181 gtgtgcatgg ccgacgatgc ggcgggcacg accgagctgc cgcaacgcct gcgccacctg
     6241 ctggagcgca gccgccgcct cttcatccgc gtggcgcgcc tcgacgagct ggcgcgccgg
     6301 cagatcgact gatctatttc ttgaggccga tgccctggaa gcgcttgttg aagcgcgcga
     6361 gctgtccgcc gtcgagcaga cgctgctgcc cgccggtcca ggcgggatgg gacttcgaat
     6421 cgatctcgag gcggagcgtg tcgcccgctt tgccgtaggt cgagcgtgtc ttgtacgagg
     6481 tgccgtcggt catgacgacg gtgatctcgt gatagtcggg atgaatgccg tctttcatca
     6541 tcgtgctccg cggaaagtag cggcgttata cccaagagaa agcgtccgcg caatcgtcgc
     6601 ccgctcccga gtcgcgggga atcgagcgaa aaaaacgtcg caacatgcca tttcgactcc
     6661 ctttccgacg actttcgcgc aaaaaaacgc tacttcaccc cgcgaaatcg caattccatt
     6721 cgactttcgc ttgcgacttt catagaatcg ccgcgtttcg tcggcacagg gggttttgaa
     6781 caccatgaaa gcaaaacgta agcgtgccaa agctgcagcc tccaagggtg cggcgaagaa
     6841 ggcgaagaag ggccgcagaa agaaaaagaa gtagctcggt cgctccccgc cgcgacgctc
     6901 ccaaagatcg aagcgtcgcg gcggcgacat ccgaacccta ggaaagccgc ggagccctgc
     6961 tccgcggctt cttaattttg gtctagcgct cggtgagctt gagctcgatg cggcgattgc
     7021 gctggtaggc gtcctcggta tcgcgcgcat cgatcggctg gaactcgccg aagccggcgg
     7081 cgaccaggtg gttcgccgga atgccctggt cgatgaagaa gcgcacgacc gagatggcgc
     7141 gcgcggaaga gagttcccag ttcgagcgga attgcggcgt cgcgatgggt cgcttgtcgg
     7201 tatggccgtc gacgcgcagc acccattcga tgtcgggcgg gatcttggcg gcgatctgct
     7261 tcagcgcctg ggccaccggc agaagctgcc gtttggcgtt gtccgagagg tcggcgctcg
     7321 ccatgccgaa gagcacctcc gactggaaga cgaagcggtc gccgacaacg cggatgtcgg
     7381 gacggtcgcc cagcacctcg cgcaagcggc cgaagaattc cgagcggtag cgcgcgagct
     7441 cctcgacctt gctggcgagc gcggcgttca agcgcttgcc gagatcggcg atctgcacct
     7501 gctggtcctt gctcttggcc tcggacgcgt cgagcgccgc cgcgagctgc gccagctgct
     7561 cgcgcagcgc cgccagttgc tggttcacct ggtcgaccgt gctctgggcg ccggcgaggt
     7621 ccgacgcgct gcggttggcg cgcgccgccg cgtcatcggc ccgcgccgac atctcggcca
     7681 gccgcgccgt cagagcgtcg cgctcgacat tggccgcctg gagctggctc gtgagctgcg
     7741 acaggtcgtg gcgcagatcc tcgctggtct tcttctcgaa ggcgagctgg tcgacgaggt
     7801 cgctgatctg gctgttgagc cgcgccagcg cctcgttgct gccatagagc gcgtcgctca
     7861 ggaagaactg gccggcggta aagatcagga gcacgaagat gatgacgatg acgagctgcg
     7921 agagcgcgtc gacgaagccg ggccagacct cgatgttcga gcgacggtta cggcgcgacg
     7981 cgagcggcat ggctcagggg cgctgcggct cttgcgccag cgccgcgatg gtgcgcgcca
     8041 ggaggcggat ctcgctccgg atctcctgga cggcctcgcc gcgcccttgc ggcagatcct
     8101 cggcgaggcg cgcgagatag acctcgatgt tgcgcagatg cccgcgcgtc tcgtcctgcc
     8161 cgccgccagc cgcgccctcg gcgagacgcg tcagcaccgg cttcaactcg ccctgctgct
     8221 cggcgagctt gagcagcagg ttctggccga tgcgcatctg gtcggcaagc gtcgtgagcc
     8281 gctcggtcag cgccagcgtg ttggcattgg cggcgatgcg gctgtcctcg ccacgcgtca
     8341 tgatgcgctg gagattctcg aggccgtccg ccgtctgctc gagtagcgcc tgaatgaagg
     8401 cgggcaccga gccgtcgccg ccttcgccgc cgagcgcgct gcccgagagc cgcgtgtagc
     8461 cggaaagcca ctcctcgagg ccgttgtaga agagattctg ggcgagcgtc gcctgcaggc
     8521 cgaggaaccc gatcaccagc gagcccgcga gaccgaagag cgaggcgctg aacgcggtgc
     8581 ccatgcccga caggatcggc atcaatcccg ccttgaagtc gctgaaggcg cgcgcggcat
     8641 ccgtggtgcc gaccgagagg ccctggacga tcgccggcat cgactgcacc gtttgcaaca
     8701 atccgtagaa agttccgagc agaccgagga acagcaacag gccccggaga tagtcggagc
     8761 tctcgcgcgt ctcgtcgagc cgcgtctgga tgccgtcgag caaggtgcgc atggacagtg
     8821 ccgagagggt catcttgccg ccgcggcgat cggcgagcat ggtggcgagc gacgacaaga
     8881 gccgcggcat gtcctcgctc ggcacattat tattctggtt gcgccggaag cgctcgatcc
     8941 agccgatctc gggatagagc aggatcacct ggcggaagat gtagacggtg ccgatggcga
     9001 gaatgaagag gatcacgcca ttgaccagcc agttgcccat gaaatagcga tagagcggct
     9061 gcgccagtac cgcagcgagg ccgagaaccg ccaccatgaa gacgaccatg cggacgagat
     9121 agcgcgacgg gcgggtcacg ctcagtgttc catcggggcg acgtcgacgc ggtcgggcgg
     9181 atcgccctcg gcggaatgat tgccgagcgc ccgcacctcc atgcgcgtcg tggcgacgcc
     9241 gtggtcggcc aggtatttac gcaccgcgag cgcgcgcgtg agcgacgtcc gccgtgcgtc
     9301 attcgcttgc tcggcgctgc ccgccgcata ggccacgacc tcgatccgcg cctgctgatt
     9361 ggtgtcgagc cattcg
//
LOCUS       DASYNS010000139         2407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2372134, whole genome shotgun sequence.
ACCESSION   DASYNS010000139 DASYNS010000000
VERSION     DASYNS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372134"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VGP50_04385"
     CDS             complement(<1..774)
                     /locus_tag="VGP50_04385"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HEV8026640.1"
                     /translation="MPLRRARNVFGVRRRRCVGVIDVGSNSLRLVIYDGIHRSPNTLF
                     NEKAMCGLGRGLESTGRLDPDGVALARVNLQRFVTLARDIGVRRLDVLATAAVRDASD
                     GQAFVQEIEKRHRLQVRVLSGAEEGRLSAFGVVAGIPDASGVVGDLGGGSVELVGVAG
                     GQVGAATTLPLGPLRLADHAGDDRKLRDVIDKHLASVSWLSSKIDGSAFYAVGGAWRA
                     LARIHMEQTRYPLHIIQQYTLPRGEAEDFLDIVARQSRKS"
     gene            complement(928..>2407)
                     /locus_tag="VGP50_04390"
     CDS             complement(928..>2407)
                     /locus_tag="VGP50_04390"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEV8026641.1"
                     /translation="FSVTGQGMFRILRDSDIEIEEEAEDLVRLFETALKRRRRGNVIR
                     LAVEASMPEDLRALLVEQLDASPDDVVPLEGLQGLADTKQLIIDERPDLIYTPFNPRF
                     PERIRDYGGDCFAAIRQKDIIVHHPYESFDVVVQFVRQAARDPAVVAVKQTLYRTSDD
                     SPIVRALIEAADAGKSVTALVELKARFDEEANIRWARDLERAGVQVVYGFLELKTHAK
                     VSLVVRREAGALRSYVHFGTGNYHPITAKIYTDLSFFTCDPALCRDAQRLFNYMTGYA
                     TPAMLEKIAVAPINLRQRLVELIEAEIAHARAGRPAQIWAKMNALVDPAIIDLLYRAS
                     QEGVEIDLVVRGICCLRPGVPGLSDNIRVKSIIGRFLEHGRIVCFGNGHKLPSTEAKV
                     FISSADWMPRNLDWRVESLVPIENETVHRQVLDEIMGANLRDQALSWRLEPEGEYVRE
                     SIDPDAFSAHTYFMTNPSLSGRGSALNRTRNGPRLVAERSEP"
BASE COUNT          415 a          778 c          811 g          403 t
ORIGIN      
        1 cgatttgcgc gattgccgcg ccacgatgtc gaggaaatcc tcggcctcgc cgcgcggcag
       61 cgtgtattgc tggatgatat ggagaggata gcgcgtctgc tccatatgga tgcgggcaag
      121 cgcccgccat gcgccaccga ccgcgtaaaa ggccgagccg tcgattttcg aggaaagcca
      181 ggacacgctc gccaagtgct tgtcgatgac gtcgcgcagc tttcggtcgt cgcccgcatg
      241 gtctgccagc cgcaaggggc cgagcggcaa ggtggttgcc gcgccgactt ggccgccggc
      301 cacgcccacg agctcgacac tgccgccgcc gaggtcgccg acgaccccac tcgcgtcggg
      361 aatgccggca acgaccccga aggcggaaag ccgaccttcc tcggcgccgc tcagcacccg
      421 cacctgaagc cggtggcgct tctcgatctc ctggacgaag gcttggccgt cgctggcgtc
      481 gcgtaccgcg gcggtcgcca gcacatcgag gcgcctgacc ccgatatcgc gtgccagcgt
      541 gacgaagcgc tgcagattga cgcgcgccag cgcgacgccg tcgggatcga gacggcccgt
      601 cgactcgagg ccgcggccga ggccgcacat cgccttctcg ttgaagagcg tgttcggcga
      661 gcggtggatg ccgtcataga tgacgaggcg cagcgagttc gagccgacat cgatgacgcc
      721 gacacagcgg cgccgcctta caccgaagac attccgcgct ctgcgcagcg gcattctagg
      781 atttaccctc gcgactcgga tagctgccgc gcaccctgac cagcgcccgc aagcggtgac
      841 ggtggttcac ggtggaattt cttcctcctc ccgctttcgg ctggaggaag gaatggagag
      901 tgcgtgacgg gccggcgatt cgccgcacta aggctccgac cgttcggcga caaggcgcgg
      961 cccgttgcgc gtgcggttga gcgcgctgcc gcggcccgaa aggcttggat tggtcatgaa
     1021 ataggtatgc gcgctgaagg cgtcgggatc aatgctttcg cgcacgtatt cgccttcggg
     1081 ctcgagtcgc caggagagcg cctggtcgcg cagattggcg cccatgatct catcgagcac
     1141 ctggcgatga accgtctcgt tctcgatcgg caccagcgac tcgacacgcc agtcgaggtt
     1201 gcgcggcatc caatcggccg aggagatgaa caccttggcc tcggtggatg gcagcttgtg
     1261 gccattgccg aagcagacga tgcggccatg ctcgaggaag cggccgatga tgctcttgac
     1321 gcggatgttg tcggagagcc cggggacccc cggcctcaag cagcagatgc cgcgcaccac
     1381 gagatcgatc tcgacgccct cctgcgacgc ccgatagagc aggtcgatga tcgccgggtc
     1441 gaccagcgca ttcatcttcg cccagatctg tgccggacgg ccggcgcgcg catgagcgat
     1501 ctcggcctcg atgagctcga ccaggcgctg gcgcagattg atcggcgcca cggcgatctt
     1561 ctcgagcatc gccggcgtgg cgtatcccgt catgtagttg aacagccgct gcgcgtcgcg
     1621 gcagagcgcg ggatcgcagg taaagaagga gagatccgta tagatcttgg cagtgatcgg
     1681 atgatagttg ccagtgccga aatgcacata ggagcggagc gccccggcct cgcggcgtac
     1741 caccaaagac accttggcgt gcgtcttgag ctcgaggaag ccgtagacca cctggacgcc
     1801 ggcgcgctcg aggtcgcgcg cccagcgaat attggcctct tcatcgaagc gcgccttgag
     1861 ctcgaccagc gccgtcaccg acttgccggc gtccgccgcc tcgatgagcg cgcgcacgat
     1921 cgggctgtcg tcgctggtgc ggtacagcgt ctgcttcacg gcgaccaccg caggatcgcg
     1981 cgccgcctgg cgcacgaact gcacgacgac gtcgaagctc tcatagggat gatggacgat
     2041 gatgtccttc tggcggatgg ccgcgaagca gtcgccgccg tagtcgcgga tgcgctcggg
     2101 gaatcgcgga ttgaagggcg tgtagatgag gtcgggccgc tcgtcgatga tgagctgctt
     2161 ggtatcggcc aggccttgaa gcccctcgag cgggacgacg tcgtcgggcg aggcgtcgag
     2221 ctgctcgacc agcagcgcgc ggaggtcctc gggcatgctt gcttcgacgg caaggcggat
     2281 cacattgcca cgccgccggc gcttgagcgc cgtctcgaag agccgcacca ggtcctcggc
     2341 ttcttcttcg atctcgatgt cgctgtcgcg caggatgcgg aacatcccct ggcccgtcac
     2401 cgagaag
//
LOCUS       DASYNS010000140         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2372231, whole genome shotgun sequence.
ACCESSION   DASYNS010000140 DASYNS010000000
VERSION     DASYNS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2372231"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="VGP50_04395"
     CDS             <1..1882
                     /locus_tag="VGP50_04395"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEV8026642.1"
                     /translation="FRVLGTFLGAAAAVLYLPAFVNEPAVLSLVLALWLGLCIYFAQL
                     DRTPRSYVFLLAGYTASIIGFPSVLAPGSIFDVAVLRVQEIGIGIAAATFVHGVVFPR
                     SVTDRLRQQITGIVGSAEQGSRLALAGKRDPQLDRERRQLASAVTGIGQLAFHVAFDT
                     ARLLPRAALIRAMQEQLCWLPPLTAAIEDRIAECTAREGGLPSDIAALVAQVESWLVH
                     DIAGPARDATARQLVAEAARLEAVFDAEATWRGREILLVSLLARLSELVLVHRLLREL
                     YDHLAGASIRGLSPEAARLIASATGRSFHRDRGLALRTALGAVVAVSGVCAFWIATAW
                     PSGAVAALIVGVGCALFGTLPQASIAIRLFFVGALIGIVAAAFFGFAIFPRVTDFVML
                     AAFMALPLLIFGSMMVRPLLAAIGLGGVLGFVNTVGLASTYQSSFTAFINGALATIAG
                     VAAATFFIDMFCVIGADVAFARLFRAGFRDIAARADGKARDAGLWTARMIDRIALVAA
                     RAGPTGVHPALPPYDALVGLRVGYLAGELRALCSTLAEGGERRAVDAALSGISFHFRA
                     IGPTKRVKMGDAVLRAIDCAITAFATDRQPERRRRSAILLLTGLRRTLFPEAEAFGGG
                     PA"
     gene            1879..2094
                     /locus_tag="VGP50_04400"
     CDS             1879..2094
                     /locus_tag="VGP50_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HEV8026643.1"
                     /translation="MIGEVSIGGVYVPTLLLLGCAAVILTGLVSRLLSLVGFYRLVAY
                     KPVTDIALFLLVLAAIVWLTEGWGLRT"
     gene            2091..3002
                     /locus_tag="VGP50_04405"
     CDS             2091..3002
                     /locus_tag="VGP50_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEV8026644.1"
                     /translation="MTSLKFLLPAFLRWSATSLLVLAAGLTALQLWDRYETAPWTRDG
                     HVFADVVRVSPDISGLVTEVAVHDNQIVQKGQLLFVVDRPRYADALAQADAAVASAQA
                     MLDQARRVAKRDRALKELVAIEAREEDDAKVLTLQGALNQALAARTTAQLNLDRTSVL
                     ASVNGYITNLSLRPGDFLGAGAQALAMIDLDSVRIEAYLEETKLRHAEIGDQARIRLM
                     GDDQDITGHVESIAGGIADDQRSTSPNLLPTVNATFTWVRLAQRIPVRIHVDAMPPGT
                     KLILGRTATVTIIPSSGKDSPSREQSP"
     gene            2999..>4023
                     /locus_tag="VGP50_04410"
     CDS             2999..>4023
                     /locus_tag="VGP50_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV8026645.1"
                     /translation="MKWRCVAAAMALAAGGCNAGPDYHVPADAAANAPEATGVFLNGD
                     SPAFAVAPLPDHWWRLYDDERLDGYVGEALAANTDLRVAEANLKRANAAIREAEAAQT
                     PGTLLFASVDKTKVGGIATLEFPDWNYSAGLAVSYPLDLAGGIRRTVEAAKADAEAVA
                     AERDEVRVTVAAAVTRNYVAVCSANFSLAAAQRVVEVESQTLDDVRRLFHEGRNTAFD
                     VTRSETPVAQSAATIPPIIAQRQAALYALAALMGRATTGYPRELEDCARPPPLRQQLP
                     IGDGAALIRRRSDVRAAERSLAAATAAIGIETAQLYPQVSIGGALGIAGPFSTIGSVS
                     SFGGSVGP"
BASE COUNT          553 a         1463 c         1304 g          703 t
ORIGIN      
        1 cttccgcgtg ctcggcacat ttctcggtgc ggccgcagca gtcctctacc ttccggcttt
       61 cgtgaatgaa ccggcggtcc tgagcttggt tctggcgctg tggctcgggc tctgcatcta
      121 cttcgcgcag ctcgaccgca cgccgcgttc ctacgtcttc ctcctcgccg gctacacggc
      181 gagcattatc ggctttccca gtgtcctcgc gccgggcagc atcttcgacg tcgcagtgct
      241 gcgtgtccag gagatcggca tcggcatcgc cgccgccacg ttcgtccatg gcgtggtctt
      301 tccgcgcagc gtcacggacc gtctccgcca gcagatcacc gggatcgtcg gcagcgccga
      361 gcaggggagc cgtcttgccc tcgccggaaa gcgcgaccct caacttgacc gcgaacgacg
      421 acagctcgcc agcgcggtca ccggtatcgg acaacttgcc tttcatgtgg ccttcgatac
      481 ggcgcggctg ctgccgcggg ccgccctgat ccgcgcgatg caggagcagc tctgctggct
      541 tccgccgctg accgccgcca tcgaagaccg catcgccgag tgcaccgcac gggagggcgg
      601 gctcccgtcg gacatcgccg ccctggttgc ccaggtcgaa agctggctcg tccatgacat
      661 cgccgggccc gcgcgcgacg cgaccgcgcg gcagctcgtc gccgaggcgg cgcggctcga
      721 agcagtgttc gatgcagagg ccacctggcg cgggcgggag atactcctcg tcagcctgct
      781 cgcccgcttg tcggagctcg tcctggtcca tcgcctgctt cgcgagctct atgaccacct
      841 cgccggcgcc agcatccgcg gcctatcgcc cgaggcggcc cggcttatcg cctcggcgac
      901 cggccggtcg ttccatcgcg accgcggcct cgcgctccgc acggcgctcg gcgccgtcgt
      961 cgcggtctcc ggcgtctgcg cgttctggat cgcgacggct tggccgagcg gggctgtggc
     1021 cgcattgatc gtcggtgtcg gctgcgcgct gttcggcacc ctcccgcagg ccagcatcgc
     1081 gatccggctt ttcttcgtcg gcgcccttat cggcatcgtg gcggcggcgt tcttcggctt
     1141 cgcgatcttt ccgcgcgtca ccgacttcgt gatgctggcc gcgttcatgg cgctgccgct
     1201 cctgatcttc ggcagcatga tggtgcgccc gttgctcgcg gcgatcggcc tcggcggcgt
     1261 cctcggattc gtcaacaccg tcggcctcgc ctcgacctac cagagcagct tcacggcctt
     1321 catcaacggg gcgttggcca cgatcgccgg cgttgccgcg gccaccttct tcatcgacat
     1381 gttctgcgtc atcggcgccg acgtcgcctt cgcccggctg ttccgtgccg ggttccgcga
     1441 tatcgcggcg cgggccgacg gcaaggcgcg cgacgcgggt ctgtggaccg cgcgcatgat
     1501 cgatcgcatc gcgctcgtcg ctgcccgcgc ggggccgacc ggtgtacatc cggcgctccc
     1561 tccctacgac gccctcgtcg gattgcgggt cggctacctt gccggcgaat tgcgggcgct
     1621 ctgctcgacc ctcgccgagg gcggggagcg ccgcgccgtc gacgcggctc tctcgggcat
     1681 cagcttccat ttccgcgcga tcggcccgac gaagcgcgtg aagatgggag acgccgtact
     1741 gcgggcgatc gattgcgcga taacggcatt cgcgacggac cggcaaccag agcgccggcg
     1801 acgcagcgcg atccttctcc tgaccggact ccgacgcacc ttgttcccgg aggccgaagc
     1861 cttcggcgga ggacccgcat gatcggcgaa gtctcgatcg gcggtgtcta cgttccgacg
     1921 ctgctgctcc tcggttgtgc ggcggtgatt ctcaccgggc tcgtctctcg actcctgtcg
     1981 ctggtcggct tctaccggct ggtggcgtac aagccggtga cggatatcgc cctctttctg
     2041 ctggtgcttg ccgcgatcgt ctggctcacc gagggttggg ggcttcgtac atgacgtcgc
     2101 tcaaatttct gctgccggcc ttcctgcgct ggtccgccac gtcgctgctc gtcctggccg
     2161 ccggcctgac cgctctccag ctctgggacc gctacgagac ggccccctgg acgcgcgacg
     2221 gccatgtctt tgccgatgtg gtccgagtct cgccggacat cagcggcctc gtcaccgaag
     2281 tggcggttca cgacaaccag atcgtgcaga aggggcaact cctcttcgtg gtcgaccgtc
     2341 cccgctatgc cgatgcgctc gcacaagccg acgctgccgt cgccagcgcc caggcgatgc
     2401 tggaccaggc gcggcgcgtg gccaaacgcg accgcgccct caaagagctc gtcgccatcg
     2461 aggcgcgcga ggaagacgac gccaaggtcc tgacgttgca gggcgcgctc aatcaggcgc
     2521 tggccgcgcg gacgacggcc cagctcaacc tcgatcgcac gtccgtgctc gccagcgtca
     2581 atggctatat caccaacctg tcgctgcgcc ccggcgactt cctgggcgcc ggcgcccagg
     2641 cgctcgccat gatcgatctc gattcggtcc gcatcgaggc gtatctcgaa gagacgaagc
     2701 tccgccacgc cgaaatcggc gaccaggcgc ggatccgcct tatgggcgac gatcaggata
     2761 tcaccggcca tgtcgaaagc atcgccggcg gcatcgccga cgaccagcgc agcaccagcc
     2821 ccaaccttct gcccacggtc aacgcgacct tcacctgggt gcggctcgcg cagcgaattc
     2881 ccgtccggat ccatgtcgac gcgatgccgc cgggcacgaa actcatcctc ggacgcaccg
     2941 ccactgtcac gatcattccg tcttccggaa aagactcgcc gtcgcgcgag caatcgccgt
     3001 gaaatggcga tgcgtggcgg cggcgatggc gctcgccgct ggtggctgca acgcgggacc
     3061 ggactatcat gttcccgctg acgccgccgc caacgcgccg gaagccacgg gggttttcct
     3121 caatggcgac agtccggcct tcgccgtggc gccgctcccc gatcactggt ggcggctcta
     3181 tgacgatgag cggctcgacg gctatgtcgg cgaggcgctg gcggcaaaca cggatctgcg
     3241 ggtggccgag gcgaacctga agcgcgccaa cgccgcgatc cgcgaggccg aggcggcgca
     3301 gactcccggc accctcctat tcgccagcgt cgacaagacc aaggtcggcg gcatcgccac
     3361 gctcgagttt cccgactgga attattcggc aggtctcgcc gtgtcctatc cgctcgatct
     3421 cgccggcggc atccgccgca cggtcgaggc ggcaaaggcc gatgccgaag ccgttgcggc
     3481 ggagcgagac gaggtgcgcg tgacggtcgc cgcggccgtg acgcggaact atgtagccgt
     3541 ctgctccgcc aatttctcgc ttgccgccgc gcagcgtgtc gtcgaggtcg agagccagac
     3601 cttggacgac gttcgccgcc tcttccacga aggccgcaac accgctttcg acgtgacccg
     3661 ctccgagacc ccggtcgctc agagcgcggc gacgatcccg ccgatcatcg cccagcgcca
     3721 agccgccctt tatgcgctcg cggcgctgat ggggcgcgcg accaccggct atccgcgcga
     3781 gctcgaggat tgcgcgcggc cgccgccact gcgccagcag ctgccgatcg gcgacggtgc
     3841 cgccttgatt cgccggcgca gcgacgtgcg tgccgccgag cgcagcctcg cggcagcgac
     3901 cgcagcgatc ggcatcgaga cggctcagct ctatccgcag gtcagcatcg gcggtgcgct
     3961 cggcattgcc ggacctttct cgacgatcgg ctcggtgtcg agcttcggcg gcagtgtcgg
     4021 acc
//
LOCUS       DASYNS010000141        19613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3619881, whole genome shotgun sequence.
ACCESSION   DASYNS010000141 DASYNS010000000
VERSION     DASYNS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 19613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19613
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3619881"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VGP50_04415"
     CDS             complement(<1..417)
                     /locus_tag="VGP50_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026646.1"
                     /translation="MLDRRSLLGGLALAAIAPSACAAPAPDPWRRWAVSDETQGRHID
                     FAAWNEFLARYLVAGEDGINRVAYGRVLPADHEALSLQVAALAGLPITAYRRAEQQAY
                     WLNLYNALTVRVVLDHYPVASIRDIKPGGPLSFGPWS"
     gene            complement(555..2012)
                     /locus_tag="VGP50_04420"
     CDS             complement(555..2012)
                     /locus_tag="VGP50_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV8026647.1"
                     /translation="MTLPPFVRSLSARLLVLTAIFVMVSEVLIFVPSVARFRLTYLQQ
                     RLADAHIAILALEATPDNMVSHDLAQELLDHVGAYGIVLHKPNATMMLDADPPPPIDV
                     TCDLRTDGLLDHVWEVIEMLAAPRPRVMRVLDTAPRDPLVTVEVLLNEGPMRAELLDF
                     ASRVLQLSVLISLVTGALVYLALQWLLVTPLRRLTGSMVAFRANPEDESRVIVPSKRG
                     DEVGLAERELAEMQETVRQALRHNARLAALGTAVTKINHDLRNILSTARLMSDSLADS
                     EAPEVKRVTPSLLAAIDRAVDLCTGTLSYTREGAPPLRRSRFALASLVEEIAEAVELR
                     GQSEVHLVNQVTPGLTVEADRDQLFRVIMNLAGNAVECGAHRITVGAQAGDDSLVIDV
                     GDNGPGLPPKARDNLFRPFAGSARPGGTGLGLAIAREIMRAHGGDIILADSSATGTAF
                     CLRLPQAPDETAAERTSREPSVVRTKPVQSSVGSD"
     gene            complement(2183..3613)
                     /locus_tag="VGP50_04425"
     CDS             complement(2183..3613)
                     /locus_tag="VGP50_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV8026648.1"
                     /translation="MTERIAADICVIGAGSGGLSVAAGASQLGMKTVLVERGKMGGDC
                     LNYGCVPSKSLLAAAKRVRAARMAGAFGLSETVAAVDFPAVMRHVHEVIAAIAPQDSV
                     ERFEGLGVRVVKAEARFAGPRAVAAGDCVVAARRIVIATGSAPSLPPIPGLTDVKFLT
                     NETVFENQILPEHLIVIGGGPIGLEMAQAHRLLGARVTVLEAARVLAKDDPELAALLV
                     ERLRGDGVVIEEGVKIRRVEGAAARVAVVVEEAGGERRIEGSHLLVAAGRRANLAGLA
                     LDQAGIAVNERGALVLDGRLRTSNRRVYAVGDAAGGPQFTHVAGYHAGIVIRNALFRL
                     PAKVDYRALPWVTYTEPELAQVGMTEAEALAAHKGDVAVLRWPFHENDRAQAERETHG
                     LVKALTRRSGEILGASIIGPQAGELIQVWGLAISSRLKLGAVRDYMAPYPTLGEVNKR
                     AAGSFYTPKLFSDRTRRLVRFLARFG"
     gene            complement(3610..4341)
                     /locus_tag="VGP50_04430"
     CDS             complement(3610..4341)
                     /locus_tag="VGP50_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV8026649.1"
                     /translation="MNEAQGRSSGFRRWLPLLVLAAAIAAVFAAGLGHYLTWEAFRDN
                     REWLLDAIARMGIVAPLIYILVYTAVAALSVPGAAVLTLMGGFLFGTWLGGLYCLIGA
                     TIGGSIVFLIARTSLGDLLRERAGPALRKLEAGFREDSVSYLLFLRLVPLFPFWLVNL
                     VPAFFDVSLRTFVLCSFIGMAPGSFVYSSVGAGAGALIAEGQTPDLHIIFQWQVLGPL
                     VALAALALVPVVYKRIKNRQEQIAP"
     gene            4460..4594
                     /gene="rpmH"
                     /locus_tag="VGP50_04435"
     CDS             4460..4594
                     /gene="rpmH"
                     /locus_tag="VGP50_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV8026650.1"
                     /translation="MKRTYQPSKLVRKRRHGFRARMATVGGRKVLASRRAKGRKRLSA
                     "
     gene            4619..4996
                     /gene="rnpA"
                     /locus_tag="VGP50_04440"
     CDS             4619..4996
                     /gene="rnpA"
                     /locus_tag="VGP50_04440"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV8026651.1"
                     /translation="MSPLPRLKRRAEFLRIAGSGRKSVTPGLILQVAPRPVATAEARA
                     SLPALRVGFTASRKVGIAVERNRARRRLRAAVAQVMALHAAPDRDYVLVARAATVRRP
                     FAALKGDLETALRQTGAYRDAPS"
     gene            5024..5278
                     /gene="yidD"
                     /locus_tag="VGP50_04445"
     CDS             5024..5278
                     /gene="yidD"
                     /locus_tag="VGP50_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV8026652.1"
                     /translation="MVSLVLRGLIRAYQLFLSPLVGPTCRYLPSCSDYAATAIRRHGA
                     VAGSWLALKRLLRCHPWGGSGYDPVPPAPPRVSPACRHSH"
     gene            5286..7019
                     /gene="yidC"
                     /locus_tag="VGP50_04450"
     CDS             5286..7019
                     /gene="yidC"
                     /locus_tag="VGP50_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV8026653.1"
                     /translation="MDEQQRNLIIAVALSIAILLGFQFFFGQQQHPPVLPSATQTSEQ
                     QQAAEPNAAAPTAPAGVAPVRPRDEVLAETPRVGIETPRLKGSINLVGAGIDDLTLLT
                     YHQTTDPESPPIRLLSPEGSEHPYVADFGWAPSQGVKLPDATTHWTASSTILTPDHPV
                     ELTWDNGAGLRFIRRFAVDQDYMFTITETVENHGESPVTLYPYGRVDRTGNPVTSSTY
                     ILHEGPIGVFNGTLNDGIAYKDLKPGDARGFATTGGWMGFTDKYWLVSLVPDQQEPVK
                     ARFLRADHDRNEIFQADYTGSEHVVPPQGTLSIDDHLFAGAKILSLLDNYESTLHIPL
                     FDHAIDFGWFDFLTKRIFQALDYFNRVLGNFGLAIMLLTVIFKLLFFPLANKSYRAMS
                     KMKLLQPEMAKLKERFGEDKERLNQETMALYKRVGANPVAGCLPIVVQIPVFFALYKV
                     LYVTIEMKGAPFYGWIHDLSVPDPTSFVNLFGLLPYAVPHIPALASVLNIGLWPMIMG
                     VTMFLQQKLNPPPADPVSAKMFTFLPLFFTWMLAQFPAGLVIYWTWNNLLSIAQQWVI
                     MRRAGVKHPTR"
     gene            7028..7720
                     /gene="yihA"
                     /locus_tag="VGP50_04455"
     CDS             7028..7720
                     /gene="yihA"
                     /locus_tag="VGP50_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEV8026654.1"
                     /translation="MPRATAAALPVIHPSDAAPDAAAEEAGRRLFAGRCEFVAGAASE
                     AALPPASLPAIAFAGRSNVGKSSLLNAITGRAALARVSHRPGRTQQLNFFDLEHRLML
                     VDLPGYGFAEVSRRDVERWTSLIRRYLRGSASLRRTLLLIDARHGIKEPDRPMMELLD
                     ETAVSYQAVLTKIDKVAPAELAERVAGVAAELATHGAAHPVIHLTSAHDGIGIAALRA
                     GIAALAAPKPAG"
     gene            7734..8642
                     /gene="argB"
                     /locus_tag="VGP50_04460"
     CDS             7734..8642
                     /gene="argB"
                     /locus_tag="VGP50_04460"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEV8026655.1"
                     /translation="MTDTPSPPTIDPASKAATLAAALPYLRRYAGKTIVVKYGGHAMG
                     NEALGELFAQDVVLLKQVGINPIVVHGGGPQIGEMLERLKIKSSFVDGLRVTDAATVG
                     IVEMVLSGSINKQIVTAINAVGGFAIGLSGKDANLITARKLERRHIESDSHIEKVLDL
                     GFVGEPERINATVLETFERSDIIPVIAPIGVGPNGETFNINADTVAGAVAAAVKATRL
                     LLLTDVTGVLDKKKKLIHELSTEDARALIADGTIQGGMIPKIETCIEAAEGGVEAAVI
                     IDGRVPHAILLELFAEGAGTLIRGRR"
     gene            complement(8853..10415)
                     /locus_tag="VGP50_04465"
     CDS             complement(8853..10415)
                     /locus_tag="VGP50_04465"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004859.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEV8026656.1"
                     /translation="MSFIAPIIPDTAPFTVAQRAWLNGWLAAYLGAEGAAAASASSPP
                     ASSPAPPEDFPWHDAVLPLDERLRLAEGRPKPRVLMAAMAQLDCGQCGYLCRTYAEAI
                     AAGSEKSLTRCVPGGKETARALKELLELPASVPAAPAPNPPPRAAAAAAFGPAPARFE
                     AALRLNGEGSEKDTRHVVFDISAAALAYEVGDSLGVHAANCPELVAALIERLGARDAD
                     DVDCPDGSRRTLRDALRLVCDIGRPSDEAVEVLASRAPDHDESQRLQALAEGYPGAQP
                     EDADLLDLLLAFPSARPPVQELIAALGVLQPRLYSIASSPKAARGQVHLTVAAVRYRK
                     HGRLRKGIASIFLAERAARDVPVKVFVQPAHGFRLPAKDDAPVIMIGPGTGVAPFRAF
                     LQERRARGATGRNWLFFGDQRRASDFLYEEELSGYRRDGLLARLDVAFSRDQAERVYV
                     QHRMRERSAELWSWLQDGAHLFVCGAQGMARDVDQALAAIIARQGGMTSGAAKAYLAT
                     MAREERYQRDVY"
     gene            complement(10421..12199)
                     /locus_tag="VGP50_04470"
     CDS             complement(10421..12199)
                     /locus_tag="VGP50_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirA family protein"
                     /protein_id="HEV8026657.1"
                     /translation="MTGGADGFTEEQKQYLEGFLGGVIARRAAQSALPPSSAPAAATP
                     GIGPADAHRAAQDRFLAAGKQLSPEEQAKREGHPLDLWDDMRENVAAGRFPKGLDVFR
                     HKFHGLFYVAPAQNAFMCRLRIPNGILTAAQFCGIAEIARRHGGGCADVTTRANLQLR
                     EIPATQPLDVLMAIQDLGLTSRGAGADNIRNVTGSPTAGIDPAELIDTRPLARELHYY
                     ILNHRELYGLPRKFNIAFDGGGSLGVLEDTNDIGFAAVAVDDGAGVDPGVYFRLALGG
                     ITGHGDFACDTGVLLEPAECIPVAAAVLRVFIAEGDRTDRQRARLKYVLDRWGLERYL
                     AETEKLLPFALRRLPLAACRKRAPLLKHGHIGVHRQRQPGLNYIGVALPVGRLTAAQM
                     DGLASIAERQGSGTLRLTVWQNLLISDIADAAVPEAQRAIEALGLDWRATAIRGALVA
                     CTGNTGCKFSATDTKGQALALAAHLDARLALDQPVNIHVTGCPHSCAQHYVGDIGLLG
                     AKVGEDAVEGYVIYVGGGAGPERSLGREVYPALPMAEMPRRVEGLLRAYLAHRRDAAQ
                     SFHDFAAAIELDALRGFADAALDNAN"
     gene            12395..13258
                     /gene="nirC"
                     /locus_tag="VGP50_04475"
     CDS             12395..13258
                     /gene="nirC"
                     /locus_tag="VGP50_04475"
                     /inference="COORDINATES: protein motif:HMM:NF008595.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HEV8026658.1"
                     /translation="MACFLRRSAEISCEGKAMYLETVDNFATLARQKSDFLKRSPLGF
                     WISAMMAGAYVGLGIILIFSVGSDVDPGYRALVMGASFGIALTLVVFAGSDLFTGHTM
                     FMPLGLLRGKIGWSDLAASWAMSWLGNLVGAGLLAALFVAGGGGGVLHSKSGWLITVA
                     AAKMNAPATALFFRAVLCNWLVCLALWMAARMTSDSAKCIAIFWCLFAFIGSGFEHSV
                     ANMTLLSIALLGDHPDTISLMGLGYNLLWVTLGNIVSGALFVGVGYWAVSRSPERRPM
                     LVVSRDAAAGD"
     gene            complement(13272..13394)
                     /locus_tag="VGP50_04480"
     CDS             complement(13272..13394)
                     /locus_tag="VGP50_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026659.1"
                     /translation="MLWAPDPHLLPGAATEISTVGLKEAFDAFRASALPGLLPR"
     gene            complement(13512..13832)
                     /locus_tag="VGP50_04485"
     CDS             complement(13512..13832)
                     /locus_tag="VGP50_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026660.1"
                     /translation="MPLLSNRKHRLRHVVAAALVALSPLAAAAAADDGPAAPVVSERR
                     FADWTLRCEDTTCWIYDNAPREEGQRVPYAMTIGARAQDGAYRVLVHMANAPRLMRRQ
                     GIDL"
     gene            13894..15408
                     /locus_tag="VGP50_04490"
     CDS             13894..15408
                     /locus_tag="VGP50_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026661.1"
                     /translation="MARDKFRQQFLTFLRSRFGRSFLAFALLSALLSAGVGYGFYYSS
                     LQSFLRNKGEEKVTALALVDAFVADYAQARAHYLSGDAPVPQTFRAHAIDRFNRDRSP
                     GEALRMRLVGAPGREIATPAGDPSVVAAMERFAQARDPKPLNSFVTVDGEVLLRTISP
                     SLASQQNCVDCHNKLQPDKPAWHLNDVMGAFVVDVPAGAFFRHNRLHALGIGGLVFAL
                     SAALGFYILLLHFRHFAASEAAQLTLREAKEAAEAANGAKSEFLAKMSHELRTPLNAV
                     IGYSEMLLEDAEIAGNSNQTVADLKRINSAGRHLLSLVTDVLDLAKIEAGKMELALQP
                     FALDGFIDDVVATCRALIIDNGNDFVVERQGVLGTVTGDATKLRQIVLNLLSNAAKFT
                     KNGRVTLEASHQDAADGAWIAISVRDTGIGISAEHLRRLFQNFSQADASTANRYGGTG
                     LGLALSQKLCRLMNGEITVESAPGNGSCFTLWVPIEPPAVATEATRRRLDAIAG"
     gene            15710..16420
                     /locus_tag="VGP50_04495"
     CDS             15710..16420
                     /locus_tag="VGP50_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEV8026662.1"
                     /translation="MSSTTPDYDPARRGRPLAEIETWIFDLDNTLYPASCNLFVEAEA
                     RMAAFIVAELKLELEAAHALRRRFFLDHGTTLRGLMLEHDIPPERFLDYVHDIDLSPV
                     PPDPALAAAIAALPGRKFVFTNATEHYARRVLARIGLDEHFAAVHDILACDYLPKPGP
                     SGYRVLMQRHGIDPERALMVEDMARNLLPAAALGMTTAWVVTGNERAAEGTGDHVHHV
                     VDALTPWLAAALRRISAG"
     gene            16454..17296
                     /gene="dapD"
                     /locus_tag="VGP50_04500"
     CDS             16454..17296
                     /gene="dapD"
                     /locus_tag="VGP50_04500"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEV8026663.1"
                     /translation="MSDKELAATIDAAWEARDGISPATKGAVRDAVEAALDALDCGRL
                     RVAEKREGRWSVHQWLKKAVLLSFRLNDTVPIVGGPGGGGWWDKVPSKFAGWGASHFR
                     EAGFRAVPNCVVRRSAYVAPGVVLMPCFINVGAYVDSGTMIDTWSTVASCAQIGKNVH
                     ISGGVGIGGVLEPLQAEPVIVEDNCFIGARSEVAEGVIVETGAVLAMGTFIAASTPII
                     DRATGEIYRGRVPAFSVVVPGSLPGRTLPDGSPGPNLSCAVIVKRVDERTRAKTSINE
                     LLRD"
     gene            17296..18468
                     /gene="dapE"
                     /locus_tag="VGP50_04505"
     CDS             17296..18468
                     /gene="dapE"
                     /locus_tag="VGP50_04505"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEV8026664.1"
                     /translation="MTAARHNQPIDPVSLAAELIRHPSVTPKDEGAIPHLAAVLERLG
                     FACDLQEFTEAGTAPVLNLYARIGKSGRNFCFAGHMDVVPPGNLEAWTAGPFAGTVTA
                     DTLHGRGAADMKGAIACFAAAAARFLAARGRDFGGSISFLITGDEEGPRINGTRKLLE
                     WAARRGETLDACVIGEPTNVQALGDMIKIGRRGNIYGYITVEGVQGHTAYPHLADNAA
                     HRLLALLHALATAPIDQGTEHFQPSNLQIATIDIGNPVDNVIPGTARATFNIRFNDRW
                     TTATIKDWIKSRLDSVGGGYTLRYRESCDAFLTPPGALSTLLSQAAKRVTGRTPELST
                     AGGASDGCFIHRYCETAEFGLVGLTQHKVDEHVALADMAALTDIYATALDLYFPVS"
     gene            18491..19123
                     /locus_tag="VGP50_04510"
     CDS             18491..19123
                     /locus_tag="VGP50_04510"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEV8026665.1"
                     /translation="MTGAADDRHGRGAAAALRRLADEDQRPRFAVADLLSALGDQGFG
                     LLILLLALPNALPGPMIPGFSAPFALAIGLLGLQLARGFPMPRLPGWLKRRSMPQERF
                     RRFVLRTEPVLRRLERWLRPRPSGLTQGPGERLVGVALIALSAVLVLPVPFGNLPIAL
                     SIMIIALGLLEGDGRALILGLAAGLLATLWNASLIFAGAAILEAAAHLAK"
     gene            complement(19153..>19613)
                     /locus_tag="VGP50_04515"
     CDS             complement(19153..>19613)
                     /locus_tag="VGP50_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026666.1"
                     /translation="DKATIVFYRGANSADTNHAMLFDDSTNPPSLIGIINSGTKIAFV
                     GDPGTRRFMVFNERPDFMGAELAPGKTYYVELAPTGRGTWKERFALYPRRGDPDVMTQ
                     LSALSWVENTPESQNSVAPHMPSIMRRREIALPKWERRPDRPILKAEYGQ"
BASE COUNT         3215 a         6738 c         6569 g         3091 t
ORIGIN      
        1 gctccacggc ccgaaactca atggaccgcc cggcttgatg tcgcggatgc tggcgacggg
       61 atagtgatcg agcaccacgc gcaccgtcag cgcattgtag agattgagcc aataggcctg
      121 ctgctcggcg cggcgatacg cagtgatcgg caggccggca agcgccgcga cctggagcga
      181 caacgcctcg tggtcggccg gcagcacgcg gccataggcg acgcggttga tgccgtcctc
      241 gccggcgacg agatagcgcg cgagaaattc gttccaggcg gcgaaatcga tgtgccggcc
      301 ctgcgtctcg tcgctcaccg cccagcgccg ccacggatcg ggcgcgggcg cggcgcaggc
      361 ggacggcgca atcgccgcta acgccaatcc gccaagcagg ctacgccgat ccagcacggt
      421 gcctcacctt cgctctgaga gaggggtaaa caggtaaggg tagagtgttg gttctggtcg
      481 gatcaatgcg gcccgcgcga cgcgcagcgt cgtcgcgtgg taggagcgac gatcgctgcg
      541 cgatcgcgca gaggtcaatc cgagccgacg gatgactgca cgggcttggt gcggacgacg
      601 ctgggctcgc gggaggtgcg ctcggcggcg gtctcgtcgg gcgcctgcgg gagacgcaga
      661 cagaaggccg tgcccgtcgc gctgctgtcg gcgaggatga tgtcgccgcc atgggcgcgc
      721 atgatctcgc gcgcgatggc aaggccgagg ccggtgccgc ccggccgcgc cgagccggcg
      781 aagggacgga agagattgtc gcgcgccttg ggtgggagac cgggcccgtt atcgccgacg
      841 tcgatcacca ggctgtcgtc gccggcctgg gcgccgaccg tgatgcgatg cgcgccgcac
      901 tcgaccgcgt tgccggcgag attcatgatg acgcggaaca gctgatcgcg gtcggcctcg
      961 accgtcaagc cgggagtcac ctggttgacg agatgcacct ccgattggcc tctgagctcg
     1021 accgcctcgg cgatctcctc gacgaggctg gcgagcgcga agcggctgcg ccggagcggc
     1081 ggcgcgccct cgcgcgtgta gctgagcgtc cccgtgcaca gatcgacggc acgatcgatc
     1141 gccgcgagca gcgacggcgt cacccgcttc acctcgggcg cctcgctgtc ggcgaggctg
     1201 tccgacatca gccgcgccgt cgagaggatg ttgcgcagat cgtgattgat cttggtcacc
     1261 gccgtgccga gcgcggccag ccgcgcgttg tggcgcaatg cctggcgcac cgtctcctgc
     1321 atctccgcga gctcgcgctc ggcaaggccg acctcgtcgc cgcgcttcga cggcacgatg
     1381 acgcgcgatt catcctcggg gttggcgcgg aatgccacca tgctgcccgt cagccgccgg
     1441 agcggcgtca ccagcagcca ttgcagcgcc agatacacca gcgcgccggt gaccagcgag
     1501 atcaacaccg acaattgcag cacgcggctc gcgaaatcca agagctcggc acgcatcggc
     1561 ccttcgttca gcaggacctc gacggtgaca agcggatccc ggggcgccgt gtcgagcacg
     1621 cgcatgacgc gcgggcgcgg cgcggcgagc atctcgatca cctcccagac atggtccaag
     1681 aggccgtcgg tgcgcagatc gcaggtcacg tcgatcggcg gcggcggatc cgcatcgagc
     1741 atcatcgtcg cattcggctt gtggagcacg atgccgtagg cgccgacatg gtccaggagc
     1801 tcttgcgcca ggtcgtggct caccatgttg tcgggcgtcg cctcgagcgc gaggatcgca
     1861 atatgcgcat cagcgaggcg ctgctggaga taggtcagac ggaaacgcgc caccgagggg
     1921 acgaagatca gcacctcgct gaccatcacg aaaatcgccg tgagcacgag aagtcgcgcc
     1981 gaaaggctgc ggacgaacgg gggaagcgtc actcgccggc cgatcatcct atcatggtag
     2041 tacggatcgc cccggctcga ccagccgttg cccgccacat ttccatgaaa tcggatgatt
     2101 ttttttgtga gcgaatggtc gttcggctaa tccttggccg cccggacgat cagtgccacc
     2161 aagcaaaatt tacgctttcg cctcagccga agcgggcgag aaaacggacc aggcgccggg
     2221 tgcgatcgct gaagagtttc ggcgtgtaga agctgccggc ggcccgcttg ttgacctcgc
     2281 cgagggtcgg atagggcgcc atgtaatccc tgaccgcgcc gagcttgagg cggctgctga
     2341 tggcgagacc ccagacctga atgagctcgc ccgcctgggg cccgatgatc gaggcgccga
     2401 ggatctcgcc gctgcggcgc gtcagcgcct tgacgagacc atgggtctcc cgctccgcct
     2461 gggcgcggtc gttctcgtga aagggccagc gcaagaccgc gacgtcgcct ttatgggcgg
     2521 ccagcgcctc ggcttcggtc atgccgactt gggcgagctc gggctcggta taggtgaccc
     2581 acgggagcgc acggtaatcg accttggccg gcaggcggaa gagggcgttg cggatgacga
     2641 tgccggcgtg atagccggcg acatgcgtga attggggccc gcccgcggca tcgcccacgg
     2701 cgtagacgcg gcggttgctg gtgcgcagcc gcccgtcgag gacgagagcg ccgcgctcgt
     2761 tcacggcgat ccccgcctgg tccagcgcga gccccgccag gttggcgcgc cgtcccgccg
     2821 ccaccaggag atgcgagccc tcgatccgcc gctcgccgcc cgcttcctcg acgaccaccg
     2881 cgacgcgcgc ggcggcgccc tcgacgcggc gaatcttcac gccctcctcg atgacgacgc
     2941 cgtcgccgcg cagccgctcg acgagcagcg ccgcgagctc cggatcgtcc ttggccagca
     3001 cgcgtgccgc ttcgaggacc gtcacacgcg cgcccagcag gcgatgcgcc tgcgccatct
     3061 cgaggccgat cggcccgccg ccgatgacga tgagatgctc cggcaggatc tggttctcga
     3121 agaccgtctc gttggtcagg aacttcacgt ccgtcaagcc ggggatcggc ggcaggctgg
     3181 gtgcggagcc ggtggcgatg acgatgcggc gcgccgcgac gacgcaatca ccggcggcga
     3241 ccgcgcgcgg cccggcgaaa cgggcttcgg ccttgacgac gcgcacgcct aggccttcga
     3301 agcgctcgac cgaatcctgg ggcgcgatgg cggcgatcac ctcgtggacg tgacgcatga
     3361 cagcgggaaa atcgacggcg gcgacggttt ccgacaaacc gaaagcgccg gccatgcgtg
     3421 ccgccctgac gcgtttcgcg gcggcgagca gcgacttcga gggaacgcag ccataattga
     3481 ggcagtcgcc gcccatcttg ccgcgctcga cgagcacggt cttcatgccg agctgcgatg
     3541 cgccggccgc caccgacaag ccgccggagc ctgcgccgat gacgcagata tcggcggcga
     3601 tgcgctcggt cacggtgcga tctgctcctg gcggttcttg atgcgcttgt agacgaccgg
     3661 caccagcgcc agggcggcaa gtgccacgag cggccccagc acctgccatt gaaagatgat
     3721 gtggagatcg ggcgtctgtc cttccgcgat gagcgcaccg gcaccggcgc cgacgctcga
     3781 atagacgaag cttcccggcg ccatgccgat gaagctgcac agcacgaagg tgcgcagcga
     3841 cacgtcgaag aaggcgggaa cgagattgac cagccagaag gggaagagcg gaacaagccg
     3901 caggaacagg agatagctga cgctgtcctc gcgaaaaccc gcctcgagct tgcgcaaggc
     3961 gggacccgcg cgctcgcgca gcaggtcgcc caggctcgtg cgcgcgatca gaaagacgat
     4021 gctgccgccg atggtggcgc cgatcaggca atagaggccg ccgagccagg tgccgaagag
     4081 gaacccgccc atcagagtca ggacggcggc gccggggacc gacaaggccg cgaccgccgt
     4141 atagaccagg atatagatca gcggcgcgac gatccccatg cgggcaatcg cgtcgagcag
     4201 ccactcgcga ttgtcgcgaa acgcttccca ggtgagataa tggccgagcc ccgcggcgaa
     4261 gacagcggcg atggccgcgg cgagcacgag caagggcagc cagcgcctaa accccgacga
     4321 tcgaccttgc gcttcgttca tttctcggga aatccgtgtg ctgaggcggc cgggaagcgg
     4381 agcgacgttg acttaaccgg gcggctcccc tatacagcgc cgtccttccc ttgagaagtt
     4441 gtcggagttt cacgccgccg tgaagcgcac ctatcagccg agcaaactcg tccgcaagcg
     4501 ccgccatggg tttcgcgccc gcatggccac tgtcggcggc cgcaaagtcc tggcgagccg
     4561 ccgcgccaaa gggcgcaagc gcctctccgc ctgacggaga ggcgcccgtc gcggcgcgat
     4621 gagccccctt ccgcgcctga aacggcgagc agagttcctg cggatcgccg gcagcgggcg
     4681 gaagtcggtg acccccggcc tcatcctcca ggttgcgccg cgtccggttg cgacggccga
     4741 ggcgcgagcg agccttccgg cattgcgggt cggcttcacc gccagccgga aggtcggcat
     4801 tgccgtcgag cggaatcgcg cccggcggcg tctcagggcg gccgtcgcgc aggtgatggc
     4861 cctccacgca gcaccggatc gtgactatgt actggtcgcg cgggctgcga cggtgcgccg
     4921 gccctttgcc gcgctcaagg gcgatctcga gacggcgctg cggcagaccg gcgcctatcg
     4981 tgacgcacca agctaggggc ttcgagcgga gaaagcggag atcgtggtga gcctcgtcct
     5041 acgtggattg atccgcgcct atcagctttt cctgtcgccc ctcgtcgggc cgacctgccg
     5101 gtatctgccg agctgctccg actatgccgc gacggcgatc cggcgccatg gcgcggtggc
     5161 cggctcctgg ctcgcgctga agcggctcct gcgctgccat ccctggggtg gcagcggcta
     5221 cgatcccgtg ccaccggcgc cgccgcgcgt atcgccggcc tgccggcata gccactgaac
     5281 gagccatgga cgagcaacaa cgcaatctca tcatcgccgt cgcgctctcg atcgcgatcc
     5341 tgctgggttt ccaattcttc ttcggacagc agcagcaccc gccggtgctg ccgtcagcga
     5401 cgcagacgag cgagcagcag caggcagccg agcccaatgc ggcagcgccg acggcgcccg
     5461 cgggggtcgc gccggtgcgc ccccgcgacg aggtgctggc cgagacgccg cgcgtcggca
     5521 tcgaaacgcc gcgcctcaaa ggctcgatca atctcgtcgg cgcgggcatc gacgacctga
     5581 cgctcctcac ctatcaccag acgaccgatc cggagagccc gccgatccgg ctgctctcgc
     5641 ccgagggcag cgagcatcct tatgtggcgg acttcggctg ggcgccgagc caaggcgtca
     5701 agctccccga tgcgacgacg cattggaccg catcgagcac aattctcacg ccggatcatc
     5761 cggtcgagct gacctgggac aacggcgcgg gcttgcgctt catccgccgc ttcgccgtcg
     5821 accaggacta catgttcacc attaccgaga cggtcgagaa ccacggcgag agcccggtca
     5881 cgctctatcc ctatggccgc gtcgaccgga ccggcaatcc cgtgacctcc agcacctaca
     5941 tcctgcatga ggggccgatc ggcgtgttca acggcacgct caacgacggc atcgcctaca
     6001 aggacctgaa gcctggcgac gcccggggct tcgccaccac cggcggctgg atggggttca
     6061 ccgacaagta ttggctggtg agccttgtcc cggaccagca ggagccggtc aaggcgcgct
     6121 ttctccgcgc cgaccacgac cgcaacgaga tcttccaggc cgactatacc ggcagcgagc
     6181 atgtcgtgcc gccgcaagga accttgagca tcgacgatca cttgttcgcc ggcgccaaaa
     6241 tactgagcct tctcgacaat tacgagagca cgctgcacat cccgctcttc gaccacgcga
     6301 tcgatttcgg ctggttcgat ttcctcacca agcggatctt ccaggcgctc gactacttca
     6361 accgcgtgct cggcaatttc ggcctcgcca tcatgctgct cacggtgatc ttcaagctgc
     6421 tcttcttccc gctcgccaac aaatcctatc gcgccatgag caagatgaag ctgctgcagc
     6481 cggagatggc caagctgaag gagcgcttcg gcgaggacaa ggagcggctc aaccaggaga
     6541 cgatggcgct ctacaagcgc gtcggcgcca atccggtggc gggctgcctg ccgatcgtcg
     6601 tccagattcc cgtgttcttc gcgctctaca aggtgctcta cgtcaccatc gagatgaagg
     6661 gcgcgccgtt ctatggctgg atccacgacc tttcggtgcc tgacccgacg agcttcgtca
     6721 acctcttcgg cctcctgccc tacgcggtgc cgcatatccc ggcgctcgcc tccgtgctca
     6781 acatcggcct ctggccgatg atcatgggcg tgacgatgtt cctgcagcag aagctgaatc
     6841 cgccgccggc cgatccggtg agcgccaaga tgttcacctt cctgcccctc ttctttacct
     6901 ggatgctggc gcagttcccg gccgggctgg tcatctactg gacgtggaac aacctgctgt
     6961 cgatcgccca gcaatgggtg atcatgcgcc gcgccggcgt caagcatccc actcggtagg
     7021 tgatgtaatg ccgcgcgcga ccgccgcggc gttgcccgtg atccacccgt ccgacgccgc
     7081 gcccgatgcg gcggccgaag aggcggggcg ccggctcttc gcgggacgct gcgagttcgt
     7141 ggccggagcg gcgagcgaag cggcgctgcc accggccagc ctccccgcga tcgcttttgc
     7201 cgggcgctcc aatgtcggga aatcaagttt gctgaacgcg atcaccggcc gggcggcgct
     7261 ggcgcgcgta tcgcaccggc ccggccgcac gcagcaactc aatttcttcg atctcgagca
     7321 ccgcctcatg ctggtcgacc tgcccggcta cggcttcgcc gaagtatcga ggcgcgacgt
     7381 cgagcgctgg acctcgctca tccggcgcta tctccgggga agtgccagtc tccggcgcac
     7441 gctgttgttg atcgatgcgc gccacggcat caaggagccg gaccggccga tgatggagct
     7501 cctcgacgag accgccgtct cctatcaggc ggtgctcacc aagatcgaca aggtagcgcc
     7561 ggcggagctt gccgagcgcg tggcaggggt ggcggcggag cttgcgaccc atggcgcggc
     7621 gcatccggtg atccatctga cgagcgccca tgacgggatc ggcatcgccg cgctccgcgc
     7681 cggcatcgcg gcgctggcgg cgcccaagcc tgctggctag agtgccggcc gccatgaccg
     7741 acacgccgtc gccgccgacg atcgatcctg ccagcaaggc cgcgacactg gcggcggcgc
     7801 ttccctatct ccgccgctat gccgggaaga ccatcgtcgt gaagtacggc ggccatgcca
     7861 tgggcaacga ggcgctgggc gagctcttcg cgcaggatgt cgtcctgttg aagcaggtcg
     7921 gcatcaatcc gatcgtagtc catggcggcg ggccgcagat cggcgagatg ctcgagcggc
     7981 tcaagatcaa gagctccttc gtcgacggct tgcgcgtcac cgacgcggcg acggtcggga
     8041 tcgtcgagat ggtgctctcg ggctcgatca acaagcagat cgtcacggcg atcaatgcgg
     8101 tgggcggctt cgccatcggc ctgtccggca aggacgcgaa cctcatcacc gcgcgcaagc
     8161 tggagcgccg ccacatcgag agcgacagcc atatcgagaa ggtgctcgat ctcggtttcg
     8221 tcggcgagcc cgagcgcatc aacgccacgg tgctcgagac cttcgagcgc tcggacatca
     8281 ttccggtgat cgcgccgatc ggcgtcggcc cgaacggcga gaccttcaac atcaacgccg
     8341 acaccgtggc gggcgccgtc gcggcggcgg tgaaggcgac gcggctcttg ctgctgaccg
     8401 acgtgaccgg cgtcctcgac aagaagaaga agctgatcca cgagctctcg accgaagacg
     8461 cgcgggcact catcgccgac gggacgatcc agggcggcat gatccccaaa atcgagacct
     8521 gcatcgaggc ggccgagggt ggcgtcgagg ccgccgtcat catcgacggc cgcgtgcccc
     8581 atgccattct cctcgaactc ttcgccgaag gcgccggcac cctcatccgc ggccggcgtt
     8641 agatcaaggg aatgcgcgcg tcgaaggtca gggagtgttc gcaacgacgg gcgcctcgtc
     8701 cggcgccacg aagaaagaag attcaccaca gaggcacaga ggcacagagg cacagagaag
     8761 agaaactcgg acctcgcacg gcgtcggttt ttttctcttc tctgtgcctc tgtgcctctg
     8821 tgcctctgtg gtgaaacttt cttgatatcg gctcagtaga cgtcgcgctg gtagcgctct
     8881 tcgcgggcca tggtcgcgag ataggccttg gcggcgccgc tcgtcatccc gccctggcgc
     8941 gcgatgatgg cggcgagcgc ctggtcgacg tcgcgcgcca tcccttgagc gccgcaaacg
     9001 aagagatgcg cgccgtcctg gagccacgac caaagctcgg ccgagcgctc gcgcatccta
     9061 tgctgaacat aaacccgctc cgcctggtcg cgcgagaagg cgacatcgag gcgcgcgagc
     9121 aagccgtcgc gccgatatcc ggacaactcc tcttcgtaga ggaaatccga ggcgcggcgc
     9181 tggtcgccga agaagagcca gttgcggcct gtggcgccgc gggcgcggcg ctcttgaagg
     9241 aaggcgcgga acggcgccac gcccgtgccc ggcccgatca tgatgacggg cgcatcgtcc
     9301 ttcgccggca gccgaaagcc atgcgccggc tggacgaaga ctttgaccgg gacatcccgg
     9361 gcggcgcgct cggcgaggaa gatcgaagcg atccccttgc gcagccggcc atgcttgcga
     9421 tagcgcacgg cggcgaccgt cagatgaacc tggccgcgcg ccgccttggg cgacgatgcg
     9481 atcgaataga gccgcggctg gagcacgccc agcgccgcga tcagctcctg gaccggcggc
     9541 cgcgccgagg ggaaggcgag cagcagatcg aggagatcgg cgtcctcggg ctgcgcaccc
     9601 ggatagcctt cggcgagggc ctggagacgc tgcgattcgt catggtcggg cgcccgcgag
     9661 gccagtacct cgaccgcctc gtccgaggga cggccgatgt cgcagacgag gcggagcgcg
     9721 tcgcgcaacg tacggcggct gccatcggga cagtcgacgt cgtccgcgtc gcgcgccccc
     9781 agccgctcga tcaatgccgc gaccagctcg gggcaattgg cggcatggac gccgaggctg
     9841 tcgccgacct cataggcaag cgccgccgcg ctgatatcga agacgacgtg gcgcgtatct
     9901 ttctcggagc cttcgccatt gagcctgagc gccgcctcga aacgcgccgg cgccggcccg
     9961 aacgctgccg cggccgcagc gcgcggggga ggatttggcg ccggcgcggc cggcacggac
    10021 gccggcagct cgagcagctc cttcaaggcg cgtgccgtct ccttgccgcc gggcacgcag
    10081 cgcgtcaggc ttttctcgct tcccgcggcg atcgcctcgg cgtaggtgcg gcagagatag
    10141 ccgcattggc cgcaatcgag ctgcgccatc gccgccatca ggacgcgcgg cttgggccgt
    10201 ccctcggcga ggcgcaggcg ctcgtcgagc ggcaagaccg cgtcgtgcca cgggaaatcc
    10261 tccggcggcg cgggcgacga tgccggcggt gacgaagcac tcgcggccgc cgcgccctcg
    10321 gcgccgagat aggcggcgag ccagccattg agccaggcgc gctgagccac cgtaaaaggc
    10381 gccgtgtcgg ggatgatcgg cgcgatgaag ctcacttccg tcaattcgcg ttgtcgagag
    10441 cggcgtcggc gaagccgcgc aacgcgtcga gctcgatcgc ggccgcaaaa tcatgaaagg
    10501 actgtgcggc atcgcggcga tgcgcgagat aggcccgcag caggccctcg acacggcgcg
    10561 gcatttcggc catcggcagc gccggataga cctcgcggcc gaggctgcgc tcggggccgg
    10621 cgccgccgcc gacatagatg acatagccct cgaccgcatc ctcgccgacc ttggcgccga
    10681 gcagaccgat gtcgccgaca tagtgctgcg cgcaggaatg ggggcagccc gtgacatgga
    10741 tgttgacggg ctggtcgagc gccagacgcg cgtcgagatg cgccgccagc gccagcgcct
    10801 gacccttggt gtcggtggcg gagaatttgc acccggtgtt gccggtgcag gcgacgagcg
    10861 cgccgcgaat ggcggtcgcg cgccaatcga ggccgagcgc ctcgatcgcc cgctgtgcct
    10921 cggggaccgc ggcgtcggcg atgtcggaga tcagcagatt ctgccagacc gtgaggcgaa
    10981 gggtgccgct gccctgtcgt tcggcgatcg acgcgagacc gtccatctgc gccgccgtca
    11041 ggcgtccgac gggcagcgcg acgccgatat agttcagccc gggctggcgc tggcgatgga
    11101 cgccgatatg gccatgcttc agcagcggcg cccgcttccg gcacgccgca agcggcaggc
    11161 gccgcaaggc gaaggggagc agcttctcgg tctcggcgag gtagcgctcg agcccccagc
    11221 ggtcgagcac gtatttgaga cgcgcgcgct ggcgatcggt gcggtcgcct tcggcgatga
    11281 agacgcgcag cacggcggca gcgacgggaa tgcattccgc gggctcgagc aagacgccgg
    11341 tgtcgcaggc gaaatcgcca tgcccggtga tgccgccgag cgcgaggcgg aaatagacgc
    11401 cgggatcgac gcccgcgccg tcatcgacgg caaccgcggc gaagccgatg tcgttggtgt
    11461 cctcgagcac gcccagggag ccgccgccat cgaaggcgat gttgaatttg cgcggcagcc
    11521 cgtagagctc gcggtgattg agaatgtaat aatgcagctc gcgggcgagc ggccgcgtgt
    11581 cgatgagctc ggcgggatcg atgccggcgg tcggactgcc ggtgacgttg cggatgttgt
    11641 cggcgccggc accgcgcgaa gtgaggccga gatcctgaat cgccatcagc acgtcgagcg
    11701 gctgcgtcgc cggaatctcg cggagctgca ggttggcgcg cgtcgtcaca tcggcgcagc
    11761 cgccgccatg gcgccgcgcg atctcggcaa tgccgcagaa ctgcgccgcc gtgagaatgc
    11821 cgttgggaat ccgcaagcgg cacatgaagg cgttctgcgc cggtgcgacg tagaaaagcc
    11881 cgtggaattt atgccggaag acgtcgagac ccttcgggaa gcgtccggcg gcgacattct
    11941 cgcgcatgtc gtcccagaga tcgagcggat ggccttcgcg cttggcctgc tcctcgggcg
    12001 agagctgctt gccggcagca aggaagcggt cctgcgccgc acggtgagca tcggccgggc
    12061 cgatgccggg ggtcgccgcg gcgggagcgg atgacggggg caaggcactt tgcgccgcgc
    12121 gccgggcgat cactccgccc aggaaacctt cgagatattg tttctgctct tccgtgaaac
    12181 cgtcggcgcc gccggtcata ccactttgcc ttgtccccgc ccccagacga agcaagcgcg
    12241 gtgccagtcc cgcgacgtcg cgtttccggc gattccgcca ttgacgtcaa ggaattggcc
    12301 ttggctgctg cccgctcttg cagcgcctcg ctcagaattt gggcaagcct cccacgtcgc
    12361 gccgagcacc acccggacgc gcaatacgcg gggcatggca tgcttcttgc gccgcagcgc
    12421 cgaaataagc tgcgagggca aggcgatgta tctcgagacg gtcgacaatt tcgccacgct
    12481 ggcgcgtcag aaaagcgact ttctgaaacg ctcgccgctc ggcttctgga tcagcgcgat
    12541 gatggccggg gcctatgtcg ggctcggcat catcctgatc ttcagcgtcg gctccgacgt
    12601 cgatcccggc tatcgcgcgc tggtcatggg cgcgtccttc ggcatcgcgc tgacgctcgt
    12661 ggtcttcgcc ggctcggacc tcttcaccgg gcataccatg ttcatgccgc ttggcctgtt
    12721 gcgcggcaag atcggctgga gcgacctcgc cgcgagctgg gcgatgtcct ggctgggcaa
    12781 tctcgtcggc gcgggcctgc tcgcggcgct cttcgtcgcg ggcggcggcg gcggcgtgct
    12841 gcattcgaag agcggctggc ttatcaccgt ggccgcggcc aagatgaacg cgcccgcgac
    12901 ggcgctcttc ttccgcgcgg tgctgtgcaa ctggctggtc tgcctcgccc tgtggatggc
    12961 cgcgcgcatg accagcgatt ccgcgaaatg catcgccatc ttctggtgcc tcttcgcctt
    13021 catcggcagc ggcttcgagc actcggtggc caatatgacg ctgctctcga tcgcgctcct
    13081 cggcgaccat cccgatacga tttcgctcat ggggctcggc tacaacctgc tctgggtgac
    13141 gctcggcaac atcgtcagcg gcgctctctt cgtcggcgtc ggatactggg ccgtgtcgcg
    13201 gtcgcccgag agacggccga tgctggtcgt ctcgcgcgac gccgcggccg gcgactgacg
    13261 ggacgtcgcc gtcagcgcgg cagaagcccc ggcagcgcgc tcgcgcggaa ggcgtcgaac
    13321 gcctccttca gtccgacggt cgagatttcc gtcgcggcgc ccggaagcag gtgcggatcg
    13381 ggcgcccaca acaccagcgc ctcggcggcg acgatcgcct cgatcgcggc ccgcgaataa
    13441 tcggcaagga agatgcagtc gggatcgccg catctcgcat aggcgagatg tcccgccttc
    13501 tggccgtcga cctagaggtc gatgccctgc cggcgcatga gccgcggcgc gttggccata
    13561 tggacgagca cacgataggc gccgtcctgc gcccgcgcgc cgatggtcat ggcgtagggc
    13621 acgcgctgcc cctcctcgcg cggcgcattg tcgtagatcc agcaggtcgt gtcctcgcaa
    13681 cgcaacgtcc agtccgcgaa gcgccgctcg gacacgaccg gggccgccgg cccgtcatcc
    13741 gcggccgccg cggcggcgag cgggctcagg gccacgaggg cggcggcaac gacgtgcctc
    13801 aggcgatgtt tacggttgct taacaagggc ataaccaatc tggggcacgg cggggcggcg
    13861 cgcggcaaaa atcccggcct catcagaccc atcatggccc gagacaagtt tcgccagcaa
    13921 ttcctgacct tcctgcgctc acggttcggc cggagctttc tcgcattcgc cctgctgtcg
    13981 gcgttgctgt cggccggtgt cggctatggc ttctattatt cgagcctgca atcgttcctg
    14041 cgcaacaagg gcgaggagaa ggtgacggcg ctcgccctgg tcgacgcctt cgtcgccgat
    14101 tatgcccagg cccgcgccca ttacctctcc ggcgacgcgc ccgtgcccca gaccttccgc
    14161 gctcatgcca tcgaccgctt caaccgggac cgctcgccgg gcgaggcgct gcgcatgcgg
    14221 ctggtcggcg cgcccggccg cgagatcgca acgccggccg gcgatccgtc ggtcgtcgcg
    14281 gcgatggagc gtttcgcgca ggcgcgcgat cctaagcccc tgaacagctt cgtgacggtc
    14341 gacggcgagg tgctgctgcg cacgatctcg ccgtcgctgg cgagccagca aaactgtgtc
    14401 gactgccaca acaagctcca gccggacaaa cctgcctggc acctcaatga cgtgatgggc
    14461 gccttcgtcg tcgacgtccc ggccggcgct ttcttccggc acaatcggct gcacgcgctc
    14521 ggtatcggcg gcctggtctt cgcgctgagc gccgccctcg gcttctacat cctgctgctg
    14581 catttccgcc atttcgccgc gagcgaagcg gcacagctca cgctgcgcga ggccaaagaa
    14641 gccgccgagg cggcgaatgg ggccaagtcc gaattcctcg ccaagatgag ccatgaattg
    14701 cgcacgccgc tcaacgccgt gatcggctat agcgagatgc tgctcgaaga cgccgagatc
    14761 gccggcaaca gcaaccagac cgtcgccgac ttgaagcgca tcaacagcgc cggccgtcat
    14821 ctgctgtcgc tcgtcaccga cgtgctcgac ctcgccaaga tcgaggccgg caagatggag
    14881 ctggcgctcc agcccttcgc tctcgacggc ttcatcgacg acgtggtcgc gacctgccgc
    14941 gcgctcatca tcgacaacgg caacgacttc gtcgtcgagc gccagggcgt gctcggcacg
    15001 gtgaccggcg atgccacgaa gctccggcaa atcgtgctca acctgctgag caatgcggcg
    15061 aaattcacca agaacggccg cgtgacgctc gaggcgtcgc accaggatgc cgcggacggc
    15121 gcgtggatcg cgatcagcgt gcgtgacacg ggcatcggca tcagcgccga gcatctgcgg
    15181 cgcctcttcc agaatttcag ccaggccgac gcctcgaccg ccaatcgcta tggcggcacg
    15241 ggcctcggcc tcgcgctgag ccagaagctc tgccgcctga tgaatggcga gatcaccgtc
    15301 gagagcgcgc ccggaaacgg ctcctgcttc accctctggg ttccgatcga acctcccgcc
    15361 gtcgcgacag aggcgacgcg gcggcgattg gacgcgatcg cgggttagga tgcgtggaca
    15421 ttcgtgccta aacacgcgcc aaacatcccc tctcccgcat cgcgggagag ggagggaccc
    15481 attgcgcagc aatgggaggg tgagggagcg catcaggcaa agcgccttta cttgaaacct
    15541 tccctcaccc ggcgcgcgct ctgcgcgcgc caccctctcc cgcgatgcgg gagagggaag
    15601 agatgcgcat ccatgcaaat gtccgcgcat cctggcccga gcggaggttg acgcttggcg
    15661 tctcgaccgg gaaagtgagg gccgcaaaac tcaaagccca cgcgaaagca tgtcgtcgac
    15721 gactcccgac tatgacccgg caaggcgcgg ccggccgctc gccgagatcg agacctggat
    15781 cttcgatctc gacaacacgc tttatccggc ctcgtgcaat ctctttgtcg aggcggaggc
    15841 gcggatggcc gccttcatcg tcgccgagct gaagctcgag ctcgaggcgg cccatgcgct
    15901 gcgccgccgc ttcttcctcg accacggcac caccttgcgc gggctcatgc tggagcacga
    15961 catcccgccc gagcgcttcc tcgactacgt gcacgacatc gatctgagcc cggtgccgcc
    16021 ggaccccgcg ctggctgcgg ccattgccgc gctgcccgga cgcaaattcg tcttcaccaa
    16081 cgcgaccgag cattatgccc ggcgcgtcct tgcgcggatc gggctcgacg agcatttcgc
    16141 cgcggttcat gacatcctcg cctgcgacta tctgccgaag cccggccctt ccggctatcg
    16201 ggtattaatg caacgccacg gtatcgatcc cgagcgcgcc ctcatggtcg aggacatggc
    16261 gcggaacctc ctgccggccg ccgcgctcgg catgacgacg gcctgggtcg tgaccggcaa
    16321 cgagcgggcg gcggagggta cgggcgatca cgtgcatcat gtcgtcgacg cgctcacgcc
    16381 gtggctcgcc gcggcgctcc ggcggatttc cgccggttga cagcgtagcg gccctgcctc
    16441 atcgtcgcgc gccatgagcg acaaggagct tgccgccacg atcgatgcgg cgtgggaagc
    16501 acgcgacggc atctcgcccg cgaccaaagg cgcggtgcgc gacgccgtcg aagcggcgct
    16561 cgacgcgctc gattgcggcc ggctgcgcgt cgccgagaag cgcgagggac gctggtccgt
    16621 tcatcagtgg ctcaagaaag cggtgttgct gtcgttccgt ctcaacgata ccgtgccgat
    16681 cgtcggcggt cccggcggcg gcggctggtg ggacaaggtg ccgtcgaaat tcgcgggctg
    16741 gggcgccagc cacttccgtg aggccggctt ccgcgccgtg cccaattgcg tggtgcgccg
    16801 ctccgcctat gtcgcgccgg gcgtcgtgct gatgccctgc ttcatcaatg tcggcgccta
    16861 tgtcgacagc ggcacgatga tcgatacctg gtcgaccgtg gcgtcctgcg cacagatcgg
    16921 caagaacgtc cacatctccg gcggagtcgg catcggcggc gtgctcgagc cgttgcaggc
    16981 ggagcccgtc atcgtcgagg acaattgctt catcggcgcg cgcagcgaag tggcggaagg
    17041 cgtgatcgtc gagaccggcg ccgtgctggc catgggcacc ttcattgccg catcgacgcc
    17101 gatcatcgac cgcgcgaccg gcgagattta tcgcggacgc gtgccggcct tctcggtggt
    17161 cgtgcccgga agcctgcccg gcaggacgct gcccgacggc agccccggtc cgaacctctc
    17221 ctgcgcggtc atcgtcaagc gtgtcgatga gcgcacccgc gccaagacct cgatcaacga
    17281 gctgctgcgc gactgatgac ggccgcgcgt cacaaccagc cgatcgatcc cgtatcgctc
    17341 gctgccgagc tcattcgcca tccgagcgtc acgcccaagg acgagggcgc gatcccgcat
    17401 ctcgccgccg ttctggagcg gctgggcttc gcctgtgatc ttcaggaatt caccgaggcg
    17461 ggcaccgcgc ccgtgctcaa tctctatgcg cgtatcggca agagcggccg caatttctgc
    17521 tttgccggtc acatggacgt cgtgccgccg ggcaatctcg aggcctggac cgcggggcct
    17581 ttcgccggga ccgtgaccgc cgatacgctc cacggccgcg gcgctgccga catgaagggc
    17641 gcgatcgctt gtttcgccgc cgcggccgcg cgcttcctcg ccgcgcgcgg acgcgatttc
    17701 ggcggctcga tcagcttcct catcaccggc gacgaagaag ggccgcgcat caacggcacc
    17761 aggaagctgc tcgaatgggc ggcacggcgc ggcgagacgc tcgacgcctg cgtcatcggc
    17821 gagccgacca acgtccaggc gctgggcgac atgatcaaga tcggccgccg cggcaacatc
    17881 tacggctata tcaccgtcga aggcgtgcag ggccacaccg cctacccgca tctcgccgac
    17941 aacgcggcgc atcggctgct cgcgctgctg cacgcgctcg ccacggcgcc gatcgatcaa
    18001 ggcacggagc atttccagcc ctcgaacctg cagatcgcca ccatcgacat cggcaatccc
    18061 gtcgacaacg tcatccccgg cacggcgcgc gccaccttca acatccgctt caacgaccgc
    18121 tggacgaccg ccacgatcaa ggactggatc aagagccggc tcgattccgt cggcggcggc
    18181 tacacgctgc gctatcgcga gagctgcgat gccttcctga cgccgcccgg cgcgctcagc
    18241 acgctgcttt cgcaagcggc caagcgagtc acgggacgga cgcccgagct cagcaccgcc
    18301 ggcggcgctt ccgatggatg cttcatccac cgctattgcg agaccgccga gttcggcctc
    18361 gtcggcctca cccagcacaa ggtcgacgag catgtggcgc tcgccgacat ggccgcgctc
    18421 acggatatct acgcgaccgc gctcgatctc tatttcccgg tgtcctgatg cagcgcgccc
    18481 cgagccgccg gtgaccggcg cggcggacga ccggcacggc cgtggcgcag cggcagcgct
    18541 gcggcgcttg gcagacgagg atcagcgccc gcggtttgcc gtcgccgacc tcttgagcgc
    18601 gctgggcgac cagggcttcg gactcctgat cctgctgctg gcgctgccca acgcgctgcc
    18661 ggggccgatg atccccggat tctccgcgcc tttcgctttg gcgatcggat tgctgggcct
    18721 gcagctcgcg cgcggcttcc cgatgccgcg tctgccgggc tggctcaagc gccgctcgat
    18781 gccgcaggag cgcttccgcc gcttcgtgct gcgcaccgag cccgtgctgc gccgcctcga
    18841 gcgctggctc cggccgcgcc cctccggcct gacacaaggg cccggcgagc gcctcgtcgg
    18901 cgtcgcgctc atcgccttga gcgcggtgct ggtgctcccc gtgccgttcg gcaacctgcc
    18961 gatcgcgctc agcatcatga tcatcgcgct cggcctcctc gaaggcgacg gacgcgcgct
    19021 catcctcggc ctcgccgccg ggctgctggc gacgctgtgg aacgcctcgc tgatcttcgc
    19081 cggcgccgcc atcctcgagg cggcggcgca tctcgcgaaa tagtcgttgc tccccaagtc
    19141 gacgcgacgg acttactgcc cgtactcggc cttgaggatc ggcctgtccg gcctcctctc
    19201 ccatttgggc aaagcgatct cgcgccggcg catgatgctg ggcatatggg gggcgacgga
    19261 gttttgcgac tccggtgtgt tctcgaccca tgacaacgcg ctcagctggg tcatcacgtc
    19321 ggggtcgccg cgccgcggat acagcgcgaa tcgttccttc catgtgccgc gccccgtcgg
    19381 cgccagctcg acatagtagg tcttgccggg tgccaattcg gcgcccataa aatccggcct
    19441 ttcgttgaac accatgaaac gccgggtgcc gggatcgccg acaaaagcga tcttcgtgcc
    19501 ggagttgatg atcccgatga gactcggcgg gtttgtcgag tcgtcgaaga gcatcgcatg
    19561 attcgtgtcg gccgagttgg ccccgcgata gaagacgatc gtggccttgt ccg
//
LOCUS       DASYNS010000142         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_655367, whole genome shotgun sequence.
ACCESSION   DASYNS010000142 DASYNS010000000
VERSION     DASYNS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655367"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VGP50_04520"
     CDS             <1..558
                     /locus_tag="VGP50_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV8026667.1"
                     /translation="PGRRRALILDRDGVINHDLGYVHRIEDCRFIDGIFVMAKAFADR
                     GFAIVIATNQAGIGRGLYGEAEYASLRDFIAAEFGRHGVAIAAAYHCPDHPTEGVGSY
                     RRENPWRKPGPGMILQAAVDLDLDLAGSWMVGDKESDIRAGRAAGVGRLLRYDPGAPR
                     LERRDDFWIIPHLADAAGLLAAEAE"
     gene            555..1616
                     /gene="rfbB"
                     /locus_tag="VGP50_04525"
     CDS             555..1616
                     /gene="rfbB"
                     /locus_tag="VGP50_04525"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV8026668.1"
                     /translation="MSTKILVSGGAGFIGTSVVRHLLGEGVAVVNLDKLTYAGSNPPL
                     GALAAGGRYVLEVADIADREAVARIIAQHRPDAIMHLAAETHVDRSIDGPEAFVHTNL
                     VGTFTLLDAARAYWQTLSAAARSAFRFHHISTDEVFGSLGAEGRFSEDTPYRPNNPYA
                     ASKAGADHLARAWHKTYGLPVVMTNCSNNYGPWQFPEKLIPLTILNAIEGKSLPVYGT
                     GANRRDWLYVGDHAAALALVLRRGRPGESYNIGGGEERANIDLVRMLCRILDDELPQS
                     PYRPHERLIGFVEDRPGHDLRYAMDDGKLRRKLAWHPRESLETGLRKTVRWYLDNRAW
                     WEPIRSGVYRGERLGRGKS"
     gene            1613..2491
                     /gene="rfbA"
                     /locus_tag="VGP50_04530"
     CDS             1613..2491
                     /gene="rfbA"
                     /locus_tag="VGP50_04530"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV8026669.1"
                     /translation="MKGIILAGGSGTRLYPLTRALSKQLLPVYDKPMIFYPLSVLMLG
                     GVRDILVITTTSDAPLFHTLLGDGSQWGVRLTYAVQAMPRGLPDAFLVGRDFLAGEGA
                     VLMLGDNLVYGDGLTEMMRRSATRAEGATIFGYRVVDPERYGVVEFDAAGKALSLEEK
                     PKKARSDWAVIGLYFYDRDVVKIAETLKPSARGELEITDLNNAYLRAGKLYLERLGRG
                     YAWLDTGTHESLIEASEFVRTLEKRTGQKIGCLEEVAFRMGFIDAAQMKRLIQLYNKS
                     DYGRYLERLLDEGGRS"
     gene            2577..>2819
                     /locus_tag="VGP50_04535"
     CDS             2577..>2819
                     /locus_tag="VGP50_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026670.1"
                     /translation="MKQYLAAAVVLVALSAPAFANHCPKDMAAIDAALAKNPPLSEAQ
                     LTEVKKLRAEGEALHKAGKHQESVDALQKAEDILKIP"
BASE COUNT          479 a          941 c          921 g          478 t
ORIGIN      
        1 cccggccgcc gccgcgcgct gatcctcgat cgcgacggcg tcatcaacca tgatctgggc
       61 tatgtccatc gcatcgagga ttgccgcttc atcgacggca tcttcgtcat ggccaaggcc
      121 tttgccgatc gcggcttcgc cattgtcatc gccaccaacc aggcggggat cggccgcggt
      181 ctctatggcg aggccgaata tgcgagtttg cgcgatttca tcgccgccga attcggccgc
      241 catggcgtcg ccatcgccgc cgcctatcac tgccccgatc atccgacgga aggcgtgggc
      301 tcctatcgcc gggagaaccc ctggcgcaag cccgggccgg gcatgatcct gcaggcggcc
      361 gtcgacctcg acctcgatct cgcgggctcc tggatggtcg gggacaagga gagcgacatc
      421 cgcgccggcc gtgccgccgg ggtcggccgc ctgctacgct atgatcccgg agcgccgcgg
      481 ctcgagcgcc gtgacgattt ctggatcatc ccgcatctcg ccgatgcggc ggggctgctc
      541 gcggcggagg cggaatgagc acgaaaattc tggtgagcgg cggcgcgggc ttcatcggca
      601 cctcggtggt gcgccatctg ctgggcgagg gcgtggcggt cgtcaatctc gacaagctca
      661 cttacgccgg ctcgaacccg ccgctgggcg cgctcgcggc gggcgggcgt tatgtcctcg
      721 aagtcgcgga tatcgccgac cgggaagcgg tcgcgcgcat catcgcgcag caccggccgg
      781 acgccatcat gcatctcgcc gccgagactc atgtcgaccg ctcgatcgat gggcccgagg
      841 ccttcgtgca taccaacctc gtcggtacct tcacgctcct cgacgcggcg cgtgcctact
      901 ggcagacgct gtcggccgcc gcgcgaagcg cgttccgctt ccatcacatc tcgaccgacg
      961 aggtgttcgg ctcgctgggc gcggaaggcc gcttcagcga agacacgccc taccgcccga
     1021 acaaccccta cgcggccagc aaggccggcg ccgatcatct ggcacgcgcc tggcacaaga
     1081 cctacggctt gcccgtagtg atgacgaatt gctcgaacaa ttacggaccc tggcagtttc
     1141 ccgagaagct gatcccgctg acgatcctga acgcgatcga gggcaaatct ctgccggtct
     1201 atggcaccgg cgcgaaccgc cgcgactggc tctatgtcgg cgaccatgcg gcggcgctgg
     1261 cgctggtgct gcgccgcggc cgccccggcg agagctacaa catcggcggc ggcgaggagc
     1321 gcgcgaatat cgatctcgtg cgcatgctct gccggatcct cgatgacgaa ttgccgcaat
     1381 cgccttatcg gccgcatgaa cggctcatcg gcttcgtcga ggatcgcccg gggcacgatc
     1441 tgcgctatgc catggatgac ggcaagctgc gccgcaagct ggcctggcac ccgcgcgaga
     1501 gcctggagac gggcttgcgc aagacggtgc gctggtatct cgacaaccgc gcgtggtggg
     1561 aaccgatccg cagcggcgtc tatcgcggcg agcgcctggg acgcggcaaa tcatgaaggg
     1621 catcatcctc gccggcggct cgggcacgcg gctctacccg ctgacgcgcg cgctctcgaa
     1681 gcagctgctg ccggtctacg acaagccgat gatcttctat ccgctctcgg tgctcatgct
     1741 gggcggcgtc cgcgacattc tcgtcatcac gacgacgagc gacgcgccgc tcttccacac
     1801 gctgttgggc gacggcagcc agtggggcgt gcgcttgact tacgccgtgc aggcgatgcc
     1861 gcgcgggctt ccggacgcgt tcctcgtggg gcgcgatttc ctcgccggcg agggcgcggt
     1921 gctcatgctt ggcgacaacc tcgtctatgg cgatggcctt accgagatga tgcgccgctc
     1981 ggccacgcgt gccgagggtg ccaccatctt cggctatcgc gtcgtcgatc ccgagcgcta
     2041 tggcgtcgtc gaattcgatg ccgcgggcaa agcgctctcg ctcgaggaga agccgaagaa
     2101 ggcgcgttcc gactgggccg tgatcgggct ctatttctat gatcgcgacg tcgtgaagat
     2161 cgccgagacg ttgaagccgt cggcgcgcgg cgagctcgag atcaccgatc tcaacaatgc
     2221 ctatctgcgc gccggaaagc tctatctgga gcggctcggc cgcggctatg cctggctcga
     2281 caccggcacg catgaatcgc tgatcgaagc gtcggaattc gtgcgcaccc tcgagaagcg
     2341 caccggccag aagatcggct gtctcgaaga ggtggcgttc cgcatgggct tcatcgatgc
     2401 ggcgcagatg aagcgcctga tccagctcta caacaagtcc gactacggac gatacctcga
     2461 gcggctcctc gatgagggtg ggcgatccta gcccgcactc ttcacgcgaa tgtgtgaggc
     2521 ggaaatcggc agcttgctgc taccaatgcc gcgccaacaa cgggaggata aaactaatga
     2581 aacagtatct cgccgccgct gtcgtgctcg tcgctctgtc ggcgccggca ttcgccaatc
     2641 attgcccgaa ggatatggcc gcgatcgatg cggcgctcgc caagaacccg ccgctgagcg
     2701 aggcgcagct gacggaggtc aagaaactgc gcgccgaggg cgaggcgttg cacaaggccg
     2761 gcaagcatca ggaatcggtc gacgcgctgc aaaaggccga ggacatcctg aagatcccg
//
LOCUS       DASYNS010000143         3546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3464440, whole genome shotgun sequence.
ACCESSION   DASYNS010000143 DASYNS010000000
VERSION     DASYNS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3464440"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VGP50_04540"
     CDS             <1..222
                     /locus_tag="VGP50_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEV8026671.1"
                     /translation="RQLEIVLGHYEAMLSHYGSAVGSRIARKHLAWYSKGLPGSAEFR
                     AAVNRTDDVGAARALIRGFYEPLLERQAA"
     gene            219..1340
                     /locus_tag="VGP50_04545"
     CDS             219..1340
                     /locus_tag="VGP50_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026672.1"
                     /translation="MSKSSIAAKRSEALRAAAIDTAAVLGALPDPVLVVDGAGRLRFA
                     NAAAEQFLDTGVATFLGAPLSELLPADSPVFSLIASVRESGHSVSEYGVTIETPRLGC
                     HVVTVQAAPIAEDRDDVVITLQERSIAHKIDRQLVHRNAARSVTAMAAMLAHEVKNPL
                     SGIRGAAQLLEQNASAGDRELTRLICDESDRICALVDRMEVFSDQRPIERGRANIHEV
                     LNRVRLVAQSGFARHVRFVEDYDPSLPPVHGNRDLLIQLFLNLVKNAAEAVPAQGGEI
                     QITTAYRHGVRLAVAGSDSRVHLPLVVEIIDNGEGIPEELAQHLFDPFVSTKRNGSGL
                     GLALVAKVIGDHGGVIEFESQPRRTMFRVCLPLAPRETV"
     gene            1354..2802
                     /gene="ntrC"
                     /locus_tag="VGP50_04550"
     CDS             1354..2802
                     /gene="ntrC"
                     /locus_tag="VGP50_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEV8026673.1"
                     /translation="MSSASTILIADDDRAIRTVLSQALGRLGHDVRTTGNAATLWRWV
                     SDGEGDLVITDVIMPDENGLDLVPRIKKLRPELRVIVMSAQNTLLTAVKANERGAFEY
                     LPKPFDLRELVSAVERALAVQAAGPAAALDPGEAEEQLPLIGRSPAMQEIYRVLARLM
                     GTDLTVMISGESGTGKELVARALHDYGKRRSGGFVAINMAAIPRELIESELFGHEKGS
                     FTGATARGVGRFEQAQGGTLFLDEIGDMPLEAQTRLLRVLQQGEYTTVGGRTPIRADV
                     RIIAATHRDLRHLIRQGLFREDLFYRLNVVPIRLPPLRERAEDIPALVRHFFAQAHAE
                     GLPLKSLDQAAMERLKAHRWPGNVRELENLVRRLAALYSQEVIGLDAIEAELAEAAPV
                     PSPAEAPANEGLGGAAERHLRDYFAAHKGGLPPAGLYDRVLREVEKPLILIALGATRG
                     NQLKAAQLLGLNRNTLRKKIRELDIDVARNVK"
     gene            2929..3381
                     /gene="mscL"
                     /locus_tag="VGP50_04555"
     CDS             2929..3381
                     /gene="mscL"
                     /locus_tag="VGP50_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV8026674.1"
                     /translation="MLKEFRDFAVRGNVVDLAVGIVIGAAFGKIVTSFVSDILMPPLG
                     LLLGRVDFSNLFLNMSGTHYGSLAEAKAAGAATLNYGLFINTILDFVIVAFAVFLLVK
                     QVNRLTRKPAEAPQAPTTKECPYCVSTIPLRATRCPNCTSSVQAAPTG"
BASE COUNT          560 a         1262 c         1173 g          551 t
ORIGIN      
        1 cgccagctcg agatcgtgct gggccattac gaggcgatgc tgtcgcatta cggcagcgcc
       61 gtcggcagcc gcatcgcgcg gaagcacctc gcctggtatt cgaagggcct gcccggctcg
      121 gccgagttcc gcgccgcggt caaccgcacc gacgatgtcg gcgccgccag ggcgctcatc
      181 cgcggcttct acgaacccct cctcgagcgg caggcggcat gagcaagagc agcatcgccg
      241 ccaagcggtc ggaggcgctg cgcgccgccg ccatcgacac ggctgccgtt ctgggcgcgc
      301 tgcccgatcc ggtgctggtc gtcgacggcg cgggccgcct gcgcttcgcc aatgccgcgg
      361 ccgagcagtt cctcgacacc ggcgtcgcga ccttcctggg cgcgccgctg tccgagctgc
      421 tgccggccga cagcccggtc ttttccctca tcgccagcgt gcgcgagagc ggccacagcg
      481 tttccgaata cggcgtcacc atcgagacgc cgcgcctcgg ctgccatgtc gtgacggtgc
      541 aggcggcacc catcgccgag gaccgcgacg acgtcgtcat cacgctgcag gagcgctcga
      601 tcgcccacaa gatcgaccgc cagctcgtgc atcgcaacgc cgcgcgctcg gtgacggcga
      661 tggcggcgat gctggcgcat gaggtgaaga acccgctctc cggcatccgc ggcgcggcac
      721 agcttctgga gcagaatgcc agcgccggcg accgcgagct gacgcggctc atctgcgacg
      781 agagcgaccg catctgcgcc ctcgtcgacc gcatggaggt gttctccgac cagcggccga
      841 tcgagcgcgg ccgcgccaac atccacgaag tgctgaaccg cgtgcgcctc gtcgcgcaat
      901 cgggcttcgc ccgccatgtg cgcttcgtcg aggattacga tccctcgctg cccccggtgc
      961 acggcaaccg cgacctgctg atccagctgt tcctgaacct cgtcaagaac gccgccgagg
     1021 ccgtgccggc gcaaggcggc gagatccaga tcaccaccgc ctaccgccac ggcgtgcgtc
     1081 tggccgtcgc cggcagcgac agccgcgtcc acctgccgct ggtcgtcgag atcatcgaca
     1141 acggcgaagg catccccgaa gagctggcac agcatctctt cgatcccttc gtcagcacca
     1201 agcgcaacgg cagcggcctc ggcctggcgc tcgtcgccaa agtgatcggc gaccatggcg
     1261 gcgtcatcga attcgagagc cagccgcggc gcacgatgtt ccgcgtctgc ctgccgctgg
     1321 cgccacgcga gactgtctga aggagggcgg ggcatgagca gcgcgtccac catcctcatc
     1381 gccgacgacg accgcgccat ccgcaccgtg ctgagccagg cgctggggcg cctcggccat
     1441 gacgtgcgca ccaccggcaa tgccgcgacg ctgtggcgct gggtctcgga cggcgagggc
     1501 gacctcgtca tcaccgacgt catcatgccc gacgagaacg gcctcgacct cgtgccgcgc
     1561 atcaagaagc tgcggcccga gctccgcgtc atcgtcatga gcgcgcagaa cacgctgctg
     1621 accgccgtca aggccaatga gcgcggcgcc ttcgagtatc tgcccaagcc cttcgatttg
     1681 cgcgagctgg tgagtgcggt cgagcgcgcg ctcgccgtgc aggcggcggg cccggcggcc
     1741 gcgctcgacc ccggcgaagc cgaagagcag ctgcccctca tcggccgctc gcccgccatg
     1801 caggagatct accgcgtgct ggcgcggctc atgggcaccg acctcacggt gatgatcagc
     1861 ggcgagagcg gcaccggcaa ggagctggtg gcgcgggcgc tccatgatta cggcaagcgg
     1921 cgctccggcg gcttcgtcgc catcaacatg gcggcgatcc cgcgcgagct catcgagagc
     1981 gagctcttcg gccatgagaa gggctccttc accggcgcca cggcgcgcgg cgtcggccgc
     2041 ttcgagcagg cgcagggcgg gacactcttc ctcgacgaga tcggcgacat gccgctcgag
     2101 gcgcagacgc ggctgctgcg cgtgctgcag cagggcgagt acacgacggt cggcggacgc
     2161 acgccgatcc gcgccgatgt gcgcatcatc gccgccacgc atcgcgacct gcgccacctg
     2221 atccgccagg ggctgttccg cgaggacttg ttctatcgcc tgaacgtggt gccgatccgg
     2281 ctgccgccgc tgcgcgagcg cgccgaggac atcccggcgc tggtgcggca tttcttcgcg
     2341 caggcccatg ccgaaggcct gccgctcaaa tcgctcgacc aggcggcgat ggagcgcctg
     2401 aaggcgcatc gctggcccgg caacgtgcgc gagctcgaga acctggtgcg gcgcctggcc
     2461 gcgctctatt cgcaggaagt gatcgggctc gatgccatcg aggccgagct cgccgaggcc
     2521 gcacccgtgc cctcgccggc ggaggcgccg gccaatgaag ggctgggtgg tgcggccgag
     2581 cggcatctgc gcgactattt cgcggcgcat aagggcgggt tgccgcccgc cggcctctat
     2641 gaccgcgtgc tgcgcgaggt cgagaagccg ctgatcctga tcgcgctcgg cgccacccgc
     2701 ggcaaccagc tcaaggcggc gcagctcctc ggcctcaacc gcaacacgct gcgcaagaag
     2761 atccgcgagc tcgacatcga cgtcgcccgg aatgtgaagt agggttcacc acagaggcac
     2821 agagacacag agaagaagga agggtcggcg cgcgaaacgc gcgctgtctt tccttctctg
     2881 tgcctctgcg cctctgtggt tattcttctt cttcggggga gggcggacat gttgaaagaa
     2941 tttcgcgact tcgcagtgcg cggcaacgtc gtcgatctcg ccgtcggcat cgtcatcggc
     3001 gccgctttcg gcaagatcgt gacgtcgttc gtgagcgaca tcttgatgcc gccgctggga
     3061 ctgcttctcg ggcgtgtcga tttctcgaat ctgttcctca acatgtcggg cacgcattac
     3121 ggctcgctcg cggaagccaa ggcggcgggc gccgcgaccc tcaactatgg ccttttcatc
     3181 aacacgatcc tcgatttcgt catcgtcgcc ttcgcggtgt tcctcctggt gaagcaagtg
     3241 aaccggctga cgcggaagcc ggccgaggcg ccgcaagcgc caaccaccaa ggaatgcccg
     3301 tattgcgtct cgacgatccc gctccgcgcc acccgctgcc ccaactgcac ctcgtcggtt
     3361 caagcggcgc cgacgggctg acgcgcgctg tcggcgctcc ctcaccctcc cactgcttcg
     3421 ctaaagcttc gcagtgggcc ctccctctcc cgcggtgcgg gagagggtaa atagcgggtg
     3481 tccgtgtcac tccgccggct gaggggagag ggatgttatg ctaccggcgc gatagaaatg
     3541 cgtccg
//
LOCUS       DASYNS010000144         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3464523, whole genome shotgun sequence.
ACCESSION   DASYNS010000144 DASYNS010000000
VERSION     DASYNS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3464523"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VGP50_04560"
     CDS             <1..780
                     /locus_tag="VGP50_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8026675.1"
                     /translation="VGGLAAVAAALPLQGVAPLGVQLAMAAAGMAAGGAWIALVGALR
                     HYRGVNETISSLLLVYIAIALLNHLVEGPMRDPASLNKPSSPGIGDANMIGMIPGTEV
                     HWGLVFGLVAAVLAYVFLYRTTFGLAARVVGGNVRAAKMVGLPVGRLILLVCALAGAM
                     AGLAGMVEVAAVQGRANATLAVGYGYTGILVSFLARHNPLAIVPVAILLGGIGASGGL
                     LQRRLDLPDATVLVLQGIIFIAVLASEALYGRLPLFRGRTA"
     gene            784..1710
                     /locus_tag="VGP50_04565"
     CDS             784..1710
                     /locus_tag="VGP50_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8026676.1"
                     /translation="MVDSAIGWWGVPIAVFGGAIRVSTPFLFVSLGECITERSGRINL
                     GLEGTLVMGAMSGYATAYLTGSPWLGVLVAGLIGALFGMLHAFICGLPRVNDIAVGIA
                     LMLFGVGLAFFLGKPFIQPQAPILPSIDFGWWSDSPSLRAALKVNSLFLVGVALAPAL
                     LWAFNHTRWGLVLRMVGDSAEAARALGYAVNAVRMRATMLGGFLGGVGGSFLSLYYPG
                     SWNERLSSGQGITAVALVIFARWNPMLCLWASLLFGGAGALGPALQSVGVTAGYHLFN
                     AAPYILTLLILVITSSPRRIISGAPGELAIIR"
     gene            1723..2727
                     /locus_tag="VGP50_04570"
     CDS             1723..2727
                     /locus_tag="VGP50_04570"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF009803.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HEV8026677.1"
                     /translation="MSGLGGLNKSPNGVVIGMVQLQLPSVATRADLAAQTEKICAMVA
                     KARRGMSSMDLVVFPEYSLHGLSMDTNREIMCRLDGPEVAAFKKACRDNRIWGCFSLM
                     EQNPEGQPYNTGIIIDDTGELRLYYRKLHPWVPVEPWEPGNLGIPVCDGPKGAKLALI
                     ICHDGMFPEMAREAAYKGAEIMLRTAGYTAPIRHAWRVTNQSNAFCNLMVTASVCLCG
                     SDGVFNSMGEAMICNFDGTLLAEGSDMPDEIVAAEVRPDLVREARECWGVENNIYQFG
                     HRGFTAVLGGAGDCPYSYMKDLVAERYRLPWEDRVQVTDGTSCGFPVPNRTYRGHRHA
                     "
     gene            2720..3403
                     /locus_tag="VGP50_04575"
     CDS             2720..3403
                     /locus_tag="VGP50_04575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEV8026678.1"
                     /translation="MPERYVEADPYRWPYNGDLRPENTALVVIDMQTDFCGIGGYVDK
                     MGYDLSLTRAPIEPIKRVLAAMRAKGFHVIHTREGHRPDLADLPANKQWRSRRIGAGI
                     GDPGPCGRILVRGEPGWEIIADLAPQPGEPVIDKPGKGSFCATDLELMLRVGGIANLV
                     LTGITTDVCVHTTMREANDRGFECLLLADCCGATDKSNHDHALKMIKMQGGVFGAVAD
                     SRTLVQALA"
     gene            3400..>3801
                     /locus_tag="VGP50_04580"
     CDS             3400..>3801
                     /locus_tag="VGP50_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8026679.1"
                     /translation="MTPAPASPAGALGLEVVAMTKRFGSLLALDAVSLRVRAGAFHAL
                     LGENGAGKSTLVKCIMGYYRPDEGQVIVGEREEVIDSPRAAHALGLGMVYQHFTLVPN
                     MTVAENLVLSRAHLPPVIDWRKERAALAAFME"
BASE COUNT          558 a         1292 c         1255 g          696 t
ORIGIN      
        1 gtcggcggcc tcgcggcggt cgcggccgct cttccgcttc aaggggtggc accgctcggg
       61 gtgcagctcg ccatggcagc cgccgggatg gcggcaggcg gcgcatggat cgcccttgtc
      121 ggtgcgttgc ggcactatcg cggtgtcaac gagacgatct cgagcctgct cctcgtctat
      181 atcgccatcg cgctgcttaa ccatcttgtc gaaggaccga tgcgcgatcc ggcgagcctc
      241 aacaagccgt catcgcccgg gatcggcgac gccaacatga tcggcatgat ccccggcacg
      301 gaggtgcatt ggggcctggt attcgggctg gtggcggcgg tgctcgccta tgtcttcctt
      361 tatcgcacga ccttcggcct tgcggcgcgg gtggtcggcg gcaatgtgcg ggcggcgaag
      421 atggtcgggc tgccggtcgg ccgcctgatc ttgctcgtct gcgcgctcgc gggcgccatg
      481 gccgggctcg ccggcatggt cgaagtcgcc gccgtgcaag ggcgcgccaa tgcgacgctg
      541 gcggtcggct acggctatac cggcatcctc gtctccttcc ttgcccgcca caatccgctc
      601 gccatcgtcc cggtcgccat cctgctgggc ggcatcggcg ccagcggcgg gctgctgcag
      661 cggcgtctcg acctgcccga cgcgacggtc ctcgtgctcc agggaatcat cttcatcgcc
      721 gtgctggcga gcgaggcgct ctatggccgc ctgccgctct tccgcggaag gacggcctga
      781 gccatggtcg actccgccat cggatggtgg ggcgtgccga tcgccgtatt cggcggcgcc
      841 atccgcgtca gcacgccctt cctcttcgtc agcctgggcg agtgcatcac cgagcgcagc
      901 gggcgcatca atctcggcct cgagggcacg ctcgtcatgg gcgcgatgag cggttacgcc
      961 acggcctatc ttacgggatc gccctggctc ggggtgctcg tcgccggact catcggcgcg
     1021 ctcttcggca tgctgcacgc cttcatctgc ggcttgccgc gtgtcaacga catcgccgtc
     1081 ggcatcgcgc tgatgctatt cggcgtcggc ctcgccttct ttctcggcaa gcccttcatc
     1141 cagccgcagg cgccgatcct gccgtcgatc gatttcggct ggtggagcga ttcgccgtcc
     1201 ttgcgtgccg cgctcaaggt caattcgctc ttcctcgtcg gcgtcgcgtt ggcgccggcc
     1261 ttgctctggg ccttcaatca cacgcgctgg gggctcgtgc tgcgcatggt gggcgacagc
     1321 gccgaagcgg cgcgcgcctt gggctacgcc gtcaatgccg tgcggatgcg ggcgacgatg
     1381 ctcggcggct tcctgggcgg cgtcggcggc tctttcctct cgctgtatta tccgggaagc
     1441 tggaacgagc gattgtcgag cgggcaaggc atcactgcgg tggcgctggt gatcttcgcg
     1501 cgctggaacc cgatgctctg cctctgggca tcgctgctgt tcggcggcgc cggcgcgctg
     1561 ggaccggcgc tgcaatccgt gggcgtcacc gccggatatc atctcttcaa cgccgcgccc
     1621 tacattctga ccttgctcat cctcgtcatc accagctcgc cgcgccgcat catttcgggt
     1681 gcgccgggcg agctcgccat catccgataa gggaggtgta gcatgagcgg cctcgggggc
     1741 ctgaacaagt cgcccaacgg cgttgtcatc gggatggtgc agctgcagct gcccagcgtg
     1801 gcgacgcgcg ccgatctcgc ggcacagacc gagaagatct gcgccatggt cgcgaaagcg
     1861 cggcgcggca tgtcgtcgat ggacctggtg gtgtttccgg agtactcgct ccacggcctc
     1921 tcgatggaca ccaatcgcga gatcatgtgc cgcctcgatg gcccggaggt cgccgccttc
     1981 aagaaggcct gccgcgacaa tcgcatctgg ggctgcttct cgctgatgga gcagaatccc
     2041 gagggacagc cctataacac cggcatcatc atcgacgaca ccggcgagct ccgcctctat
     2101 taccgcaagc ttcacccctg ggttccggtc gagccctggg agcccggcaa tctcggcatt
     2161 cccgtttgcg acgggccgaa gggcgcaaag ctggcgctca tcatctgcca tgacggcatg
     2221 ttccccgaga tggcgcgcga ggcggcctac aagggcgctg agatcatgct gcgcaccgcg
     2281 ggctacaccg cgcccatccg ccacgcctgg cgcgtcacca accaatccaa tgccttctgc
     2341 aatctgatgg tgacggcgag cgtctgccta tgcggctcgg acggcgtgtt caattcgatg
     2401 ggtgaggcga tgatctgcaa tttcgacggc acgttgctgg cggaaggcag cgacatgccc
     2461 gacgaaatcg tcgccgccga agtgcggccc gacctcgtcc gcgaggcgcg cgaatgctgg
     2521 ggcgtcgaga acaacatcta tcagttcggc catcgcggct tcaccgcggt gctgggcggc
     2581 gccggcgatt gtccctacag ctatatgaag gacctcgtcg ccgagcgcta tcgccttccc
     2641 tgggaggatc gcgtgcaggt taccgacggc acgtcctgcg gctttcccgt tcccaatcgc
     2701 acctaccgcg gacatcgcca tgcctgagcg ctacgtcgag gccgaccctt accgctggcc
     2761 ctataacggc gatctgaggc ccgagaacac cgcgcttgtc gtcatcgaca tgcagacgga
     2821 tttctgcggc atcggcggct atgtcgacaa gatgggttac gacctgtcgt tgacgcgggc
     2881 gccgatcgag ccgatcaagc gcgtgctcgc ggcgatgcgc gccaagggct tccatgtcat
     2941 ccatacgcgc gagggccatc gccccgatct cgcggatctc ccggccaaca agcaatggcg
     3001 ctcgcggcgg atcggcgcgg gtatcggcga tccggggccg tgcggccgca ttctcgtgcg
     3061 cggcgagccc ggctgggaga tcatcgcgga tcttgcgccg caaccgggcg aacccgtcat
     3121 cgacaagccc ggcaaaggct ccttctgtgc caccgatctg gaactgatgc tgcgcgtcgg
     3181 cgggatcgcc aatctcgtgc tcaccggcat caccacggat gtgtgcgttc acaccaccat
     3241 gcgcgaggcc aatgaccgcg gcttcgaatg cctcctgctc gcggattgct gcggcgctac
     3301 cgacaagagc aatcacgatc acgcgctcaa gatgatcaag atgcagggcg gcgtcttcgg
     3361 agcggtcgcc gactcgcgga cgctggtcca ggcgctggca tgacgccggc cccggcatcg
     3421 ccggcgggcg cgctcggcct cgaagtggtg gcgatgacca agcgcttcgg cagccttctg
     3481 gcgctcgacg ccgtgtcgct gcgcgtccgc gccggcgcct ttcacgccct tctcggcgag
     3541 aacggcgcgg gcaagagcac gctcgtcaaa tgcatcatgg gctattaccg gcccgatgag
     3601 ggccaggtca ttgtcggcga gcgcgaagag gtcatcgaca gcccgcgcgc ggcgcatgcc
     3661 ctcggcctcg gcatggtcta ccagcatttc acgctggtgc ccaacatgac cgtggccgag
     3721 aacctggtgc tgtcgcgcgc ccacctgccg ccggtgatcg actggcgcaa ggagcgcgca
     3781 gcgctggcgg cgttcatgga g
//
LOCUS       DASYNS010000145         5570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3932741, whole genome shotgun sequence.
ACCESSION   DASYNS010000145 DASYNS010000000
VERSION     DASYNS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3932741"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1404)
                     /locus_tag="VGP50_04585"
     CDS             complement(<1..1404)
                     /locus_tag="VGP50_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV8026680.1"
                     /translation="MDKFGIGQPVSRFEDPRFLRGEGRYINDVNLPGQAYAVFLRAPH
                     AHARIRSLDTAVAVAAPGVLAIYTGEDVAKDGLGTIPTLLQRKRPDGSPMFAPPHPGL
                     ARGRVRFVGDPVAMIVAQTLSAAKDAAEMIEIDYETLPSVTESAAAVERGAPIVWDEC
                     PDNISNVFDAGNRAAIEAAFARAAHIVRRRYVISRVFAHYMEPRGAIGSYDRGEDRYT
                     LHADVQYPHRVRNALATYVFKIPESKIRVIAGDIGGGFGTKGWQYVEHRLVLWAARKL
                     KRPVKWASERSEAILADEHARDNVSDAELALDAEGRFLGLRVRTLASIGAYISSERNL
                     LATFANTITLVGVYAIPAAHVHVICAFANKSPTAPYRGAGRPEAIYVIERLIDDAARE
                     LGRDPVELRRRNLIPPDAMPYKNPFDAVYDCGDFGRNMEDALRLSRYEGFPARRAQSR
                     MRGKLRGIGIANAIERAA"
     gene            complement(1406..2194)
                     /locus_tag="VGP50_04590"
     CDS             complement(1406..2194)
                     /locus_tag="VGP50_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV8026681.1"
                     /translation="MSSQTASLRNVKPLRAEVSAAEWQTRIDLAAAYRLCDLYGMSDM
                     IYTHISARVPDEPRHFLINPHGMLFDEITASCLLKVDLDGTVLYQPGGEYGLQIAGHV
                     IHSALYRARPDVNAAMHTHTTAGMAVSALECGLLPLTQTALRFHGRLAYHDFNGPERD
                     LGERDRLAQDLGPHNSMILRNHGLLTVGPTVAEAFTAMYGLERSCQAQVVAMACNTPL
                     NELPEKVVEKTVGMYAPSVVRRYGLLEWPALLRRLDRQDPSFRD"
     gene            complement(2277..3134)
                     /locus_tag="VGP50_04595"
     CDS             complement(2277..3134)
                     /locus_tag="VGP50_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026682.1"
                     /translation="MAAVPFALAPLRLTRLRSRWRSAVRRLRRLQRSFHRARRALNSA
                     PRSVQILVLGISGLSVLAAANLVYHVVRKPTEMFFPLATALNKLPAETWAAYGPLFRA
                     HSTATVTPELLAALAQVEGAGNPVAHTYWRWRFSWNPFEIYRPASSAVGMYQMTDGTF
                     AEAKHYCIHHHQLVAEGPWWDMTSCWFDSLYMRVLPGDAVELTAAYLDRAIADIVSRR
                     AGAAPTEQQRQDLAAVIHLCGAGAGDAFARRGFHLRPGERCGDHEAQRYVAQVNAMKR
                     SFAQLAEAE"
     gene            complement(3298..4383)
                     /locus_tag="VGP50_04600"
     CDS             complement(3298..4383)
                     /locus_tag="VGP50_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEV8026683.1"
                     /translation="MDQKAASDPRKGRGAASNASGRFEAEQRVVFDDGWGSADAVPPR
                     LDTVYTRDSSRSIIARNDSPDISFDRSINPYRGCEHGCIYCYARPSHAYLGLSPGLDF
                     ESRIFVKENAPELLRAELAKPNYACAVIALGANTDPYQPVERKLGLTRRILDVLSEHN
                     HPVSIITKSALVQRDIDILSAMAKRRLASVAVSLTTLDRGLARVMEPRAATPERRLET
                     MAALAGAGIPVTAMTAPMIPALNDMELERLLEAAHDNGARNAGYVLLRLPLELVGLFE
                     EWLATHMPGKAKHVMSLIKDARDGKAYRAEWGTRMKGTGPYAEMLRVRFDKACHRLGL
                     NERREAFRLDTTLFRRPPKKGDQLALF"
     gene            4480..5052
                     /locus_tag="VGP50_04605"
     CDS             4480..5052
                     /locus_tag="VGP50_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV8026684.1"
                     /translation="MAIDTTELRTRQRPHGLPFRIAKLGHVVLNVSDLARSVSFYTEI
                     LGFSVSDIYPESMVPGGMAFLRCNSDHHGVALVGGLKAASGNVDLHHLAFEVSTLDEV
                     LRARDHLRRHAVPIDFEGRRRAGCQIAVEFRDPDGHRLEIFWGVDQVGSDGITRPASE
                     WKWAHSLEDAVADPVRGQDTTLQDPSLLRR"
     gene            5053..>5570
                     /locus_tag="VGP50_04610"
     CDS             5053..>5570
                     /locus_tag="VGP50_04610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV8026685.1"
                     /translation="MAAIDAPVKSLLSVIVPALDAAAVLPATLAALEEGRAGGLLGEV
                     IVVDGGSKDGTAAWAEAAGAQLTRAPRGRGAQLAAGGVAARGDWLLFLHADTQLAPGW
                     SRAAAAFIAEPQNQDRAAYMRFRLNDQSAAARRMEALVAWRCRTLALPYGDQGLLIAA
                     PLYRAIGGFRALP"
BASE COUNT          907 a         1782 c         1980 g          901 t
ORIGIN      
        1 ggcggcgcgc tcgatggcat tggcgatgcc gatgccgcgc agcttgccac gcatgcgcga
       61 ttgcgcgcgg cgcgccggaa aaccctcgta gcgcgagagc cgcagcgcgt cttccatgtt
      121 cctgccgaaa tcgccgcaat catagaccgc gtcgaacggg ttcttgtagg gcatggcgtc
      181 gggcgggatc aggttccggc gccggagctc gacgggatcg cggccgagct cgcgcgcggc
      241 gtcgtcgatc aaccgttcga tgacgtagat cgcctcgggc cgcccagcgc cgcgataggg
      301 cgcggtcggg ctcttgttgg cgaaggcgca gatgacgtgc acatgggccg cggggatcgc
      361 atagacgccg acgagcgtga tggtgttggc gaaagtcgcg agcaggttgc gctcggacga
      421 gatgtaggcg ccgatgctcg ccagcgtccg cacgcgcagg ccgaggaatc ggccctcggc
      481 atccagggcg agctcggcgt cgctcacatt gtcgcgggca tgctcgtcgg cgaggatcgc
      541 ctcgcttcgc tccgacgccc atttcaccgg acgcttgagc ttccgcgcgg cccagagcac
      601 gaggcgatgc tcgacatatt gccagccctt ggtgccgaag ccgccgccga tatcgccggc
      661 gatgacgcgg atcttgctct ccggaatctt gaagacgtat gtggcaagcg cgttacgcac
      721 acggtgcgga tattgaacat cggcgtggag cgtgtagcgg tcctcgccgc ggtcgtagct
      781 gccgatcgcg ccgcgcggct ccatgtagtg cgcgaagacc cggctgatga cgtagcggcg
      841 gcgcacgata tgggccgccc gggcgaaggc cgcctcgatg gccgcgcggt tcccggcgtc
      901 gaagacattg gagatgttgt cggggcattc gtcccagacg atgggcgcgc cgcgctcgac
      961 cgcggcggcg ctctccgtca ccgacggcag cgtctcgtaa tcgatctcga tcatctccgc
     1021 ggcatctttg gcggcgctca acgtctgggc gacgatcatc gccacgggat ctccgacgaa
     1081 gcgcacgcgc ccccgcgcga ggcccggatg cggcggcgcg aacatcggcg acccgtccgg
     1141 acgcttgcgc tgcagcagcg tcggaatcgt gccgagcccg tcctttgcga cgtcttcgcc
     1201 ggtgtaaatc gcgagcacgc ccggcgcggc gacggcaacg gccgtatcga ggctgcgaat
     1261 gcgggcatgg gcatggggtg cgcggagaaa tacggcgtag gcctggcccg gcaggttcac
     1321 gtcattgatg tagcgtccct cgccgcggag gaaccgtgga tcctcgaagc gggatacggg
     1381 ctggccgatg ccgaatttgt ccatgtcagt cgcggaaaga gggatcctgc cggtcgagcc
     1441 gccgcaacag cgccggccat tcgagcaggc catagcggcg aacgaccgag ggcgcgtaca
     1501 tccccaccgt cttctcgacc accttctcgg gcagctcgtt gagcggcgta ttgcaggcca
     1561 tggcgacgac ctgcgcctgg caggagcgct cgagcccgta catggccgtg aacgcctcgg
     1621 cgacggtggg accgacggtg aggagcccgt gattgcgcag gatcatggag ttgtgcgggc
     1681 cgagatcctg cgcgagcctg tcgcgctcgc ccaaatcgcg ctcgggcccg ttgaagtcat
     1741 gataggcgag ccggccgtgg aagcgcagcg ccgtctgcgt cagcggcagc aggccgcatt
     1801 cgagcgccga gaccgccatg ccggcggtgg tgtgcgtgtg catcgcggcg ttgacgtccg
     1861 ggcgcgcgcg gtaaagggcg ctgtggatca catgtccggc gatctggagg ccgtactcgc
     1921 cgccgggctg atagagcacc gtgccgtcga gatcgacctt gagcaggcag gatgcggtga
     1981 tctcatcgaa gagcatgcca tggggattga tcaggaagtg ccggggctcg tcgggcacac
     2041 gcgccgagat gtgggtgtaa atcatgtccg acatgccata gaggtcgcag agccgatagg
     2101 cggcggcgag atcgatccgt gtctgccatt ccgcggcgct cacctcggcg cgcaacggct
     2161 tgacgttccg aagggaggcg gtctggctgc tcatggcggt gctcctcgaa cgaggacggg
     2221 gcggcgaccg caatactaag ccgcgccggc ccgagcgggc aacgccacgc ctgtggtcac
     2281 tcggcttcgg caagctgcgc gaagctccgc ttcatcgcgt tcacctgcgc cacatagcgc
     2341 tgagcctcgt ggtcgccgca gcgctcgccc ggccgcaggt gaaagccgcg gcgggcaaag
     2401 gcgtcgccgg caccggcgcc acagagatga atgaccgccg ccaagtcctg cctctgttgt
     2461 tcggtcggcg cagcgcctgc gcggcgcgag acgatgtccg cgatagcgcg gtcgagataa
     2521 gcggcggtga gctcgaccgc gtcgcccggc agcacgcgca tatagagcga atcgaaccag
     2581 cacgacgtca tgtcccacca cgggccttcg gcgaccagtt gatgatgatg gatgcagtaa
     2641 tgcttcgcct cggcaaaagt gccgtccgtc atctgataca tgccgaccgc gctcgaggcc
     2701 gggcggtaga tctcgaacgg attccacgag aaacgccagc gccaataggt gtgagcgacg
     2761 ggattgccgg cgccctcgac ctgtgccagc gctgccagca gctcgggcgt cacggtcgcc
     2821 gtcgaatgcg cgcgaaagag gggcccgtac gcggcccagg tctctgccgg cagcttgttg
     2881 agcgcggtcg ccagtggaaa gaacatctcc gtcggcttgc ggaccacatg gtagacgaga
     2941 ttggctgctg ccagcacgct cagccccgaa atgccgagca cgaggatttg cacggagcgc
     3001 ggcgccgaat tgagggcgcg ccgcgcccgg tgaaacgagc gctgaagccg ccgaaggcgc
     3061 cgcacggcgc tgcgccagcg cgagcgaagt cgcgtcaggc gcagcggcgc gagggcaaag
     3121 ggcacggcag ccatccgagg ttccgacctt ccggcgaata tacggctatc gtaagcgcag
     3181 gagaaatcat ctcacaggaa gaatagggcg aaatttcggc tattctccga gccaaagaga
     3241 ttggcgcgtt tttggacgag ttgggaacgg ttcgccgcgt gctcagcccg agcgcgccta
     3301 gaacagcgcg agctgatcgc ctttcttggg gggcctgcgg aagagcgtcg tgtcgaggcg
     3361 aaaggcctca cggcgctcgt tgaggccgag gcggtggcac gccttgtcga agcgcacgcg
     3421 cagcatctcc gcataagggc cggtgccttt catccgcgtg ccccattcgg cgcgataggc
     3481 tttgccgtcg cgcgcgtcct tgatgaggga catcacatgc ttggctttgc cgggcatgtg
     3541 agtcgcgagc cactcctcga agagccccac cagctcgagg ggcaggcgca gcaggacata
     3601 accggcattg cgcgcgccgt tgtcgtgcgc cgcctcgagc agccgctcga gctccatgtc
     3661 gttgagggcc gggatcatgg gcgccgtcat cgcggtcacg gggatgccgg cgccggccag
     3721 cgccgccatg gtctcgagcc tgcgctccgg cgtcgcggcg cgcggctcca tgacgcgggc
     3781 gagcccgcgg tcgagcgtcg tcagcgacac ggcgaccgag gcgaggcggc gcttggccat
     3841 cgcgctgagg atgtcgatgt cgcgctgcac cagtgccgat ttggtgatga tcgagaccgg
     3901 atggttgtgc tcgctcagca cgtcgaggat gcggcgcgtc aggccgagct tgcgctcgac
     3961 cggctgatag ggatcggtgt tggcgccgag cgcgatcacc gcgcaggcat agttgggctt
     4021 ggccagctcg gcgcgcagca gctccggcgc gttctccttg acgaagatgc ggctctcgaa
     4081 atcgagcccg ggcgagaggc cgagataggc atggctcggc cgcgcgtagc aatagatgca
     4141 gccatgctcg cagccgcgat agggattgat cgagcggtcg aaggagatgt cgggcgaatc
     4201 gttccgcgcg atgatgctgc gggagctgtc gcgcgtgtag acggtgtcga gccggggcgg
     4261 cacggcatcg gcgctgcccc agccgtcatc aaagacgacg cgctgctcag cctcgaagcg
     4321 gccgctcgca ttgctggcgg cgccgcggcc cttgcgcggg tcggacgcgg ctttctgatc
     4381 catggctcaa gtgtggccgc ggctaaggaa caaatcaaga actatcgtat cgccaagccc
     4441 cggggtaaac tgcgcgcggg cgggggaggc ccgaacggca tggcgatcga tacgacggag
     4501 ctcagaactc ggcagcggcc gcacggtctg ccgttccgta tcgccaagct cggccatgtc
     4561 gtgctcaatg tcagcgatct cgcgcgctcg gtgagcttct ataccgaaat tctcggattt
     4621 tccgtctccg acatctatcc tgagagcatg gtgccgggcg ggatggcgtt cctgcgctgc
     4681 aacagcgatc accacggcgt tgccctggtc ggcgggctga aggcggcgtc gggcaatgtc
     4741 gacctgcacc atctcgcctt cgaggtttcg acgctcgacg aggtgctgcg ggcgcgcgat
     4801 catctccgcc ggcatgccgt gccgatcgat ttcgaaggac ggcgccgcgc cggctgccag
     4861 atcgcggtgg agttccgcga tcccgacggg caccggctcg agatcttctg gggtgtcgat
     4921 caggtcggca gcgacggcat cacgcgtccc gccagcgagt ggaaatgggc gcacagcctc
     4981 gaggacgccg tcgccgatcc ggtccgcggc caggacacga cattgcagga cccatcgctg
     5041 ctccggcgct gaatggcggc catcgacgct cccgtgaaat cgctgctttc ggtgatcgtg
     5101 ccggcgctcg acgctgcggc ggtgctgccg gcgacgctgg cggcgcttga ggaaggacgc
     5161 gccggcggac tactcggcga agtgatcgtc gtcgatggcg gctcaaaaga tggcacggcg
     5221 gcgtgggcgg aagcggcggg cgcgcagctc actcgggcac cgcgcgggcg cggcgcacag
     5281 cttgcggcgg gcggtgttgc ggcgcgcggc gactggctcc tcttccttca cgccgatacg
     5341 caactcgccc cgggctggtc gcgggcagcg gcggcgttca tcgccgagcc ccagaaccaa
     5401 gaccgcgccg cctatatgcg ctttcgcctc aacgatcaaa gcgcggcggc gcggcgcatg
     5461 gaagcgctcg tcgcctggcg ctgccgcacg ctcgctttgc cctatggcga tcaaggcttg
     5521 ctgatcgccg cgccacttta tcgcgccatc ggcggctttc gcgctttgcc
//
LOCUS       DASYNS010000146         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1593999, whole genome shotgun sequence.
ACCESSION   DASYNS010000146 DASYNS010000000
VERSION     DASYNS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1593999"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1845
                     /locus_tag="VGP50_04615"
     CDS             <1..1845
                     /locus_tag="VGP50_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HEV8026686.1"
                     /translation="EITRLRQLSDQSLDAAEAELTAAALPDGAEAGGNLHRAREGVMK
                     LRRDADAVLQKPKSARPAEISRAWVTAVDNVVDSVDAVLESLAGQINRSDPFIVGLMD
                     IKRLAWEVRAAAGTDRLMIGAAIAGGSGLPEDQQRQLAVLQGRVESAWRLVWVKIEEP
                     GMPVELKDAFARAKKSYFTDLAEKRKAIVADLVAGQAPSLSGTDWVRTSNPGLESLFT
                     VAKTALEVADRYAGDQASSARLELMLKLSLTLVFFGIGVYALSFVVARLTGPIAAIAE
                     TTRAVAGGDLRRVILYQDRADEIGELARALAVFRDNAQAKLRIEAAQRQEQERKEQRQ
                     RVVDQHVATFDSSIRGLLDALSKAAAEMRSTSESMSATAEQTGRQAMAVSDAATHASS
                     NVETVATATEELAASVSEIGRQVNHAAQIANQAVSETRNTDGTVQGLSEAAQRIGEVV
                     QLINDIASQTNLLALNATIEAARAGDAGKGFAVVASEVKSLATQTGKATEDIAAQVAA
                     IQTATEGAVGAIKSVGHIIQQVSEISATIASAVEEQGATTKEITRNTQEAARGTQAVS
                     VNIAGVSQGAGMTGKAADHVLTAAGELGRTADKLRAEVDSFLTRIRAA"
     gene            complement(1939..3168)
                     /locus_tag="VGP50_04620"
     CDS             complement(1939..3168)
                     /locus_tag="VGP50_04620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEV8026687.1"
                     /translation="MLLSHLLHHVVRRGSLRVIDAGGAMHTFSGDPGPQITIRLHTRA
                     LEWQLFLHPRLKLGEAFMNGTLTVEDASIYDFLDLMASNMAVAPANILTPLYNGFGRA
                     FRVFQQYNPLHRARRNVAHHYDLSDTLYELFLDADRQYSCAYFAAPDQTIEQAQANKK
                     RHIAAKLLLAPGQHVLDIGCGWGGLALYLAKECGVSVTGLTLSTEQLRVAERRAAAAG
                     LSDRVRFELRDYRETNGRYDRIVSVGMFEHVGVVHYPEFFGKVKELLADDGVALLHSI
                     GRQDGPASTNPWLRKYIFPGGYSPAMSEVVPVVERSGLWITDIEILRLHYAETLRAWR
                     TRFNANRERIRALYDERFCRMWEFYLAGSEMAFRHQGHLNFQMQFAKKIDAVPLTRDY
                     MVDWERSHRASAAERAA"
     gene            complement(3467..>3767)
                     /locus_tag="VGP50_04625"
     CDS             complement(3467..>3767)
                     /locus_tag="VGP50_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal L9 C-terminal domain-containing
                     protein"
                     /protein_id="HEV8026688.1"
                     /translation="TARDISDAVTKAGFTIGRGQVVLDKAIKTLGLYKQRVVLHPEVS
                     VTVTVNVAQSAEEAEMQSKGVDPLRLAQEAAAREPAATAAAADDGAADEAAAEEE"
BASE COUNT          661 a         1235 c         1284 g          587 t
ORIGIN      
        1 gagatcacgc ggctgcgcca gttgtccgac cagtcgctcg acgcggcgga agcggagctg
       61 acggccgcgg cgctgcccga cggggcggaa gcgggcggca atctgcaccg cgcccgcgaa
      121 ggtgtcatga agctgcggcg cgacgccgac gccgtgctgc aaaagccgaa atcggcgcga
      181 ccggctgaga tcagccgggc ttgggtcacg gctgtcgaca acgtggtcga ttccgtcgac
      241 gcggtcctgg agagcctggc gggtcagatc aaccgcagcg accccttcat cgtcggcctc
      301 atggacatca agcggctggc ctgggaggtg cgtgccgcgg ccggaaccga ccgattgatg
      361 atcggcgcag ccatcgccgg cggcagcggc ttgccggagg accagcagcg gcagctcgcg
      421 gtcctgcagg gccgggtgga atccgcctgg cggctggtct gggtgaagat cgaagagccg
      481 ggcatgccgg tcgagctcaa agacgctttc gcgcgcgcca aaaaaagcta tttcacggac
      541 ctcgccgaga agcgcaaagc gatcgtcgcc gatctcgtcg ccggtcaggc gccgtcgctc
      601 tcgggcaccg attgggtgag gacctccaac cccggtctcg aaagcctctt caccgtcgcc
      661 aagacggctt tggaagtcgc cgacaggtat gccggggatc aggcgagcag cgcgcgactg
      721 gagttgatgc tgaagctcag cctcaccctc gtctttttcg gcatcggcgt ctatgcgttg
      781 agcttcgtcg tcgcccgcct caccggcccg atcgcggcga tcgccgagac gacacgcgcc
      841 gtcgccggcg gcgatctgcg ccgcgtcatt ctctatcagg accgcgccga cgagatcggc
      901 gagctggccc gcgctctcgc ggtgttccgc gacaacgcgc aggcgaagct gcgcatcgaa
      961 gcggcgcagc ggcaggagca ggagcgcaag gagcagcggc agcgcgtcgt cgaccagcac
     1021 gtcgcgacgt tcgacagctc gatccgtggg ttgctcgacg ctctctccaa ggccgcggcc
     1081 gagatgcgca gcacctccga aagcatgtcg gcaacggccg agcagacggg acggcaggcg
     1141 atggcggtga gcgatgccgc gacccatgca tcgagcaacg tcgagaccgt ggcgacggcc
     1201 accgaggagc tggccgcctc cgtttccgag atcggccgcc aggtgaacca tgccgcgcag
     1261 atcgccaatc aggcggtgag cgagacgcgc aataccgacg gcaccgtgca gggtctgtcg
     1321 gaggccgcgc agcgcatcgg cgaagtggtg cagctcatca acgacatcgc ctcccagacg
     1381 aacctcctgg cgctcaacgc gacgatcgaa gcggcgcgcg cgggcgatgc cggcaaaggc
     1441 ttcgccgtcg tcgcgagcga ggtcaagtcg ctggcgaccc agaccggcaa ggcgaccgag
     1501 gacattgctg cgcaggttgc tgccattcag acggcgacag agggcgccgt cggcgccatc
     1561 aagagcgtcg ggcacatcat tcaacaggtg agcgagattt ccgccacgat cgcctctgcg
     1621 gtcgaggagc agggcgccac gaccaaggag atcacgcgca acactcagga agccgcgcgc
     1681 ggtacgcaag cggtctccgt gaacatcgcc ggcgtgagtc agggtgccgg gatgaccgga
     1741 aaggcggcag accacgtact gactgccgcg ggcgagctcg gccgcacggc cgataagctc
     1801 cgcgccgaag tcgactcctt cctcacgcgc atccgcgcgg cctagttcag ataccagcta
     1861 tcaggtatca ggtatcaggt atcaggccgc gacacctggc tcccgctgat ccctgatccc
     1921 tgacatctga cctctgattc aagcggcccg ttcggccgcc gaggcgcggt ggctgcgttc
     1981 ccagtcgacc atgtagtcgc gagtcagcgg caccgcatcg atcttcttcg cgaactgcat
     2041 ctggaaattg agatggccct ggtggcggaa ggccatttcc gaacccgcga gatagaactc
     2101 ccacatccgg cagaagcgct cgtcgtagag cgcgcggatg cgctcgcggt tggcattgaa
     2161 gcgcgttcgc caggcgcgca acgtctcggc gtaatggagc cgcaggatct cgatgtcggt
     2221 gatccacagg cccgagcgct cgaccaccgg caccacctcc gacatcgcgg gcgagtagcc
     2281 gcccgggaag atgtatttcc gcagccacgg attcgtgctg gccggcccgt cctgacggcc
     2341 gatcgagtgg aggagggcga cgccgtcatc ggcgagcaac tccttcacct tgccgaagaa
     2401 ttcggggtaa tggacgacac ccacatgctc gaacatgccg accgacacga tgcggtcgta
     2461 gcggccattg gtctcgcgat agtcgcgcag ctcgaagcgc acgcggtcgg agagaccggc
     2521 ggcggccgcg cggcgctcgg cgacgcgcag ctgctcggtc gacagcgtga ggccggtaac
     2581 gctgacgccg cattccttcg caagatagag cgcgagcccg ccccagccgc agccgatatc
     2641 gagcacgtgc tgacccggag cgagcagcag cttggcggcg atgtgccgct tcttgttggc
     2701 ctgcgcctgc tcgatcgtct ggtcgggtgc cgcgaaatag gcgcaggagt attgccggtc
     2761 ggcgtcgagg aagagctcgt agagcgtgtc cgacaagtcg tagtgatgcg ccacgttccg
     2821 tcgcgcccga tgcagcggat tatattgctg gaacacgcgg aaggcgcggc cgaagccgtt
     2881 gtagagcggc gtcaggatgt tggccggcgc caccgccatg ttggatgcca tcaggtcgag
     2941 gaagtcgtag atgctggcat cctcgaccgt cagcgtgccg ttcatgaacg cttcgccgag
     3001 cttcagccgt ggatgcagga aaagttgcca ttcgagggcg cgagtgtgca accttatggt
     3061 gatctgcggg cctggatcgc ccgaaaaggt gtgcatcgcg ccgcccgcat cgattacgcg
     3121 caggcttccg cggcgaacta cgtgatggag cagatgcgac aacagcatcg agccgtcctc
     3181 ccgcctaccc ctgtactatc cgcaattcga tcaatccgca caagccttta ccgatcgggg
     3241 gaatggccga ggccccggcg gcggcgggaa agggccgccg agcgtggcaa gcgcgggctg
     3301 ggcgggtgcg ccggcggcgc cggcatttgg agctggattt agacaacgct gagggcgcgg
     3361 agctggcgcc gagggtcgca gaggacaaac gctctgcgac ctctacgcca cctccgcgct
     3421 ctcagcgttc ctctgcgaat tcttgcaggg cggtccgcgg cgccggttac tcttcctcgg
     3481 ccgccgcctc gtcggccgct ccgtcgtccg ctgccgccgc cgtcgcagcc ggctcgcgcg
     3541 cggccgcttc ctgggcgagc ctgagcgggt cgaccccctt cgactgcatc tccgcttcct
     3601 cagcggattg cgcgacgttc accgtcacgg tgacggagac ctccggatgg aggacgacgc
     3661 gctgcttgta gaggccgagc gtcttgatcg ccttgtcgag cacgacctgg ccgcgcccaa
     3721 tggtgaaccc cgccttggtc acggcatcgg agatgtcgcg tgccgtg
//
LOCUS       DASYNS010000147         2454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4557509, whole genome shotgun sequence.
ACCESSION   DASYNS010000147 DASYNS010000000
VERSION     DASYNS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4557509"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VGP50_04630"
     CDS             <1..878
                     /locus_tag="VGP50_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEV8026689.1"
                     /translation="WYGKAIARLQTRLIGDPTSWRYQAAIHDYNPATDPFRSPQDQLP
                     PDSDRFWQKCQHSGAYFLPWHRMYLLYFEEIIAAAVVDLGGPAGWSLPYWNYSDASNP
                     DAQKLPPAFRASSANGAANPLYVAARAPGADDGAPLGDELDVDLQCLEDSVFFGEGNG
                     AGGGFGGPNTRNPVHNGSQLGALEAVPHGSMHVAVGGSGRRAGWMSDFTTAALDPIFW
                     LHHANIDRLWQVWLRRDPQHVNPTDSTWLGQSFEFHDSTGAIVSLAPRQVLDTTAPPL
                     GYQYEDVTDPFATGA"
     gene            881..1363
                     /locus_tag="VGP50_04635"
     CDS             881..1363
                     /locus_tag="VGP50_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026690.1"
                     /translation="MASSPAEMVGATDQPVTLAANRTTADLAVRQPTGPAKQALAATA
                     KRPHAYLNLENITSSGVPASYAVYLNLPDNADPKQNGHLLAGLLPAFGVAEASRADKH
                     SAGSGLHHALNVTKVIEELEQRGQWNPQKLTVTFIPRNAGSDPIPVKVGRVSLYYKDP
                     "
     gene            1360..2130
                     /locus_tag="VGP50_04640"
     CDS             1360..2130
                     /locus_tag="VGP50_04640"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEV8026691.1"
                     /translation="MSLVPAGRGAPRGTGRDALATLAAFASRHPEWWTLLLSVAAWAA
                     VILASADRLPIALCGARRAETAPAGEIGGFMVMVAAMMLPLLVAPVRRTAFASLWCRR
                     HRAIGGFLLGYAALWLMLGGTVIGLARILCPADAALGQIASLVLAAAALWELTPGKRR
                     ALSACHRTIPLRPLGAKADVDCIRYGLLHARSCLASCWAMMLGASLASQQLPVMVAVA
                     AILMAERHWPRRTRHVDALILAGLALASALITLFEFRL"
     gene            complement(2160..2378)
                     /locus_tag="VGP50_04645"
     CDS             complement(2160..2378)
                     /locus_tag="VGP50_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026692.1"
                     /translation="MATAFGGICSSMVRTAGSIGRSANCFRFILGVSLALTLVPLLRR
                     APPLAPMRIAALGIAALAAFVLQFFGAS"
BASE COUNT          385 a          895 c          801 g          373 t
ORIGIN      
        1 gctggtatgg caaagcgatt gcacggctgc agacgcgcct gatcggcgat cccacgagct
       61 ggcggtatca ggccgccatc cacgactaca atccggccac cgatcctttc cgctcgccgc
      121 aggaccagct tccgcccgac agcgaccgct tctggcagaa atgccagcat agcggcgcct
      181 acttcctgcc ctggcatcgc atgtatcttc tctatttcga ggagatcatc gccgccgccg
      241 tggtcgatct cggcggtccc gcgggctggt cgctgcccta ttggaactac agcgacgcca
      301 gtaatcccga cgcccagaag cttccgccgg ccttccgcgc cagcagcgcc aacggggcgg
      361 ccaatccgct ctacgtcgcg gcccgcgcgc ccggcgccga tgacggcgcg ccgctcggtg
      421 acgagctcga tgtcgacctg caatgtctcg aggacagcgt cttcttcggc gagggcaatg
      481 gcgcaggcgg cggcttcggc ggacccaata ccaggaaccc ggtccacaac ggatcgcaac
      541 tgggcgcgct cgaggcggta ccgcatggct cgatgcatgt ggcggtcggc ggcagcggac
      601 gaagggccgg ctggatgagc gacttcacca ccgccgcgct cgaccccatc ttctggcttc
      661 atcacgccaa tatcgaccgg ctctggcagg tctggctgcg ccgcgacccg cagcatgtga
      721 acccgacgga cagcacctgg ctcggccaat ccttcgaatt ccatgacagc accggcgcga
      781 tcgtgtcgtt ggccccacgg caggtcttgg atacgacggc accgccgctc ggctatcaat
      841 acgaagacgt caccgacccg tttgccactg gagcatgacc atggccagct cacccgccga
      901 gatggtcggc gccaccgacc agcccgtgac cctcgccgcc aaccggacga cggccgatct
      961 cgccgtgcgc cagccgaccg gacccgcgaa gcaggcgctt gccgcgacgg caaagcggcc
     1021 gcacgcctat ctcaatctcg aaaacatcac cagcagcggc gttcccgcga gctacgccgt
     1081 ctacctcaat cttcccgaca acgccgaccc gaagcagaac ggccatctgc tcgcgggcct
     1141 gctgccggct ttcggcgttg ccgaggcgtc gcgcgccgac aagcacagcg ccggcagcgg
     1201 tctccatcat gccctcaatg tgaccaaggt catcgaggag ctggagcagc gcgggcaatg
     1261 gaatccgcag aagctgaccg tcaccttcat cccgcgcaat gcgggcagcg atccgatccc
     1321 ggtgaaggtg ggccgcgtga gcctctacta caaagacccg tgagcctcgt cccggcgggc
     1381 cggggcgcgc cgcgcgggac cggacgcgac gcgctcgcga ccctcgcggc attcgcttcc
     1441 cgccatcccg aatggtggac cctgctcctc tccgtcgcgg catgggcagc ggtcatcctg
     1501 gcgagcgccg atcggctccc gatcgcgttg tgcggagcgc ggcgcgccga gaccgcgccg
     1561 gccggcgaga tcggcggctt tatggtcatg gtcgcggcga tgatgctgcc cctcctggtc
     1621 gcgccggtcc ggcgcaccgc ctttgccagc ctttggtgcc gccgtcaccg cgcgatcggc
     1681 ggatttctgc tcggctatgc ggcgctctgg ctgatgctcg gcggcaccgt catcgggctc
     1741 gcgcgcattc tttgcccggc cgacgcagcg ctcgggcaga tcgcgtcgct ggtgcttgcg
     1801 gcggccgcgc tttgggagct gacgcccggc aagcgccgcg cgctcagcgc ctgccatcgc
     1861 accatcccgc tgcggcctct gggtgccaag gccgatgtcg actgcatccg ctacggtctt
     1921 ctccacgcgc ggagctgcct cgcgagctgc tgggcgatga tgctgggcgc gagcctcgcg
     1981 tcgcagcagc tgccggtcat ggtcgccgtc gccgcgatcc tgatggccga gcgccactgg
     2041 ccgcgccgga cgcgccatgt cgacgcgctg attctcgccg gccttgcgct cgccagcgcg
     2101 ctcattactc tgttcgagtt ccgcctgtaa cggcgcgcgc tccgggccgg cagcacgcct
     2161 taggaggctc cgaagaactg gaggacgaag gcggctaagg cggcgatgcc gagcgcggcg
     2221 atccgcatcg gcgcgagcgg cggcgcgcgc cgcagcagcg gcacgagcgt cagcgccagg
     2281 ctgacgccga ggatgaagcg gaagcaattg gcgctccggc cgatggatcc agccgtccgg
     2341 accatggacg agcagatgcc gccaaaagct gtagccacta ccgaggatcc agagcgccag
     2401 cgtcggcagc ggcagcagcg cccaggccgg cgaccggtct gggcacgccc agct
//
LOCUS       DASYNS010000148         1758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1437266, whole genome shotgun sequence.
ACCESSION   DASYNS010000148 DASYNS010000000
VERSION     DASYNS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437266"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VGP50_04650"
     CDS             complement(<1..501)
                     /locus_tag="VGP50_04650"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8026693.1"
                     /translation="MAKVAFVGLGVMGYPMAGHLRAKGGHEVTVYNRTAAKAEKWVAQ
                     HGGRSAPTPKAAAQGQDFVMACVGNDHDLRQVMLGQDGIFAGVEKGAIVVDHTTASAV
                     IARELHAEAKKGGFDFIDAPVSGGQAGAENGVLTVMCGGDEGPFARAEKVIVAYARAC
                     TLLGASG"
     gene            754..>1758
                     /locus_tag="VGP50_04655"
     CDS             754..>1758
                     /locus_tag="VGP50_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEV8026694.1"
                     /translation="MAALKDEVLARLAAVPSPDGRPLTATGKLSDILVTDGKVFFSLT
                     VDAAHVQQWESVRERAQAAVRALPGVESVMIALTAERGGGHAPHALSQPSPASGGGKS
                     ESGAAGATSPRSALRRPPMAPGARPGEGAPHSHGPWHGAAGIPGVAAIIAVASGKGGV
                     GKSTVAVNLALALHDLGLKVGVLDADIYGPSLPKLLAIRERPEVISGTRLKPIVRHGM
                     PVMSIGFLVEEETPMIWRGPMVMSALTQMLREVEWGTLDVMVVDMPPGTGDAQLTMAQ
                     QVPLKGAVIVSTPQDLALIDARRGIAMFRRVNVPVLGIVENMSTFICPQCGARSDIFG
                     H"
BASE COUNT          258 a          621 c          606 g          273 t
ORIGIN      
        1 gccggaggcg ccgagcagcg tgcaggcgcg ggcgtacgcc acgatcacct tttcggcgcg
       61 ggcgaacggg ccctcgtcgc cgccgcacat caccgtgagc acgccgttct cggcgccggc
      121 ctggccgccg gagaccggcg cgtcgatgaa gtcgaagccg cccttcttcg cctcggcgtg
      181 aagctcgcgg gcgatgaccg ccgaggcggt ggtgtggtcg acgacgatcg cgcccttctc
      241 gacgccggcg aagatgccgt cctggccgag catcacctgg cggagatcgt ggtcgttgcc
      301 gacgcaggcc atgacgaagt cctggccttg cgctgccgcc ttcggtgtcg gcgcgctgcg
      361 gccgccatgc tgggccaccc acttttccgc cttggcggcg gtgcggttgt agaccgtcac
      421 ctcatggccg cctttcgccc tgagatgccc cgccatcggg tagcccatca cgccgagccc
      481 gacaaacgcc accttcgcca ttcgtgtcct ccgtcagtag cccgcatgag cggagcgaaa
      541 tgcgggaaat gattgggccg ttgcaatgga ggtcccggat gtcgcttcgc tcatccgggc
      601 tacaggtcag gtatagcagg cccgcggcgg atcatggcaa ttgagccgcc gtgcgcgctt
      661 ccctatctac gttgcatcaa cgaggcgcgg gagcgatagg tcggcgcgat ccaggccgga
      721 tttcgcccgc gcgatgccgc gcaggaggcg aagatggcag cgctcaagga cgaagttctg
      781 gctcgcctcg ctgccgtgcc aagccccgac ggcaggccgc tcaccgcgac cgggaagctt
      841 tccgacatcc tcgtcaccga cggcaaggtt ttcttctccc tcaccgtcga tgccgcacac
      901 gtgcagcaat gggagagcgt gcgcgagcgc gcgcaggccg cggtgcgcgc gctgccgggg
      961 gtcgagtcgg tcatgatcgc gctcaccgcc gagcgcggcg gcggccatgc cccccatgcc
     1021 ctctcccaac cctcccccgc aagcggggga gggaaaagcg aaagcggcgc ggccggtgcg
     1081 acctcgccgc gcagcgcgct gcggcggccg ccgatggccc cgggtgcccg gcccggagaa
     1141 ggggcgcccc acagccacgg gccttggcac ggcgccgccg gcattcccgg cgttgccgcg
     1201 atcatcgcgg tcgcctccgg caagggcggc gtcggcaagt cgacggtggc ggtcaatctc
     1261 gcgcttgcgc ttcacgacct cggtctcaag gtcggcgtcc tcgatgccga catctacggg
     1321 ccgtcgctgc caaaactcct cgccatccgc gagcggccgg aggtgatcag cggcacgcgg
     1381 ctcaagccca tcgtgcgcca cggcatgccg gtgatgtcga tcggcttcct cgtcgaggag
     1441 gagacgccga tgatctggcg cgggccgatg gtgatgtcgg cgctgacgca gatgctgcgc
     1501 gaggtggaat ggggaaccct cgacgtcatg gtggtcgaca tgccgccggg aaccggcgac
     1561 gcgcagctca ccatggcgca gcaggtgccg ctcaagggcg ccgtcatcgt ctcgacgccg
     1621 caggacctcg cgctcatcga cgcgcggcgc ggcatcgcca tgttccgccg cgtcaacgtg
     1681 ccggtgctcg gcatcgtcga gaacatgtcg accttcatct gcccgcaatg cggggcgcgc
     1741 tcggatattt tcggccac
//
LOCUS       DASYNS010000149        11680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_345540, whole genome shotgun sequence.
ACCESSION   DASYNS010000149 DASYNS010000000
VERSION     DASYNS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345540"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VGP50_04660"
     CDS             complement(<1..486)
                     /locus_tag="VGP50_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV8026695.1"
                     /translation="MSPRPLLIDAGRRLALLAGVLAVVAVTLAVTGGLAWRGVGVVLV
                     AYGLITAGVVAGLGYHPYRRFGAANVVTLTRAAYAALLVGILSDGTASLSETGRWALV
                     AAGAAALALDGVDGWLARRSHLASVFGARFDMETDALSVLALSALVYRSGQVGAWVLT
                     AG"
     gene            822..1553
                     /locus_tag="VGP50_04665"
     CDS             822..1553
                     /locus_tag="VGP50_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HEV8026696.1"
                     /translation="MDDIPAALPDGLEGAIATDGFAFLDGATMRALLEKAGPLGDRER
                     FATSWDDMPVDAYMADGGRYRRRRHAVYAVTADGAILRQSHQPHYQSRDYNPLHGGVE
                     RWFAPIEPAIGDDPCLLTILGFCRGLFGRLAPEVRAWRVELHQFRIEARPGEAGHPTP
                     EGMHRDGVDYVLVLLIRRRNIRRGTTTIHSLEGKLLGSFTLAAPFDAALVHDARVYHG
                     VTPVEPVDPALPAFRDVLVVTFRRT"
     gene            1742..2539
                     /locus_tag="VGP50_04670"
     CDS             1742..2539
                     /locus_tag="VGP50_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEV8026697.1"
                     /translation="MSGVPDDAELWQRVLALRGAGATTVLTEAQRQPGAGLAALLPLY
                     LPLVQWPRDRCGAVAHLGQSLDGRIATAAGASRWVTGEADLLHTHRMRALADAVVVGA
                     GTVRHDDPQLTVRRCLGAQPVRVVIDSERRLGADYRIFQDGAAPTLVLAAADRVRPGE
                     RLGRAEILPLPRGADGGLAPGEIRRVLAARGLALLFIEGGGITISRFLMAGALDRLQL
                     TVAPVILGSGRPSLSLPEIAEPCQGLRPRLRRFSLGEDMLYECIFRD"
     gene            2532..3512
                     /locus_tag="VGP50_04675"
     CDS             2532..3512
                     /locus_tag="VGP50_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEV8026698.1"
                     /translation="MTERGEGRAFWISAPGRGELRSAILPALGPGDVLVRALASGISR
                     GTEALVFGGHVPPSQYVAMRCPFQEGDFPAPVKYGYASVGVVEAGPGALLGRRVFCLH
                     PHQDRYVVSADAVLAVPEAVPDERAVLAANMETAVNGLWDAAPLLGDHIAVVGAGVVG
                     SLMAALAARLPGTRVELVDIDQTRAVLAAALGCGFARPADATGDADLVIHASGSAAGL
                     ATALRLAGFEATVLEMSWYGDAPVTAPLGEAFHAKRLSLRASQVGAVATARRARWSRR
                     QRLALALELLADPVFDHLLSGSSSFADLPRTMARLADDPRGALCHVVRYG"
     gene            3571..3978
                     /locus_tag="VGP50_04680"
     CDS             3571..3978
                     /locus_tag="VGP50_04680"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEV8026699.1"
                     /translation="MYSLAVSDHVMIAHSFVGEIFGPAQQLHGATYAVEMELRRAQLD
                     SNGLVCDIGLALAMLKDVLGEINYRNLDDIPAFRGRNTTTEFLAGEIFRRCEDRIAGG
                     ALGPGTAGALASMRVTLRESPVAWAACDGALGR"
     gene            3975..5021
                     /locus_tag="VGP50_04685"
     CDS             3975..5021
                     /locus_tag="VGP50_04685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV8026700.1"
                     /translation="MTIVFIVPGPLGQLTGGYLFDKRVIEGLRAAGRAVDVRELPGAF
                     FPSADAAALAAADAVLSALPEEDAAIIDGLALPAFAQVLTPTSRRRLIGFVHHPLARE
                     TGLAEDARETLAGLEARLLTRLKGVLCPSAGTAWALEEYGVARERIAIAPPGTEKPAE
                     PARRSPRAGPLRLLAVGTISPRKGHLLLVEALAEVADRPWELSCIGSLTRDPATLAAL
                     ERAIGRHGFGERVKLLGERPPEQLAEAYAAADVFVLPSYHEGYGMAFAEALAHGLPVV
                     ATSAVAETVPAEAALLVPPGDVAALAAALRLLMDNAPLRARLAMGAAKAGAALPDWQT
                     AIDRWIAAFDRLSA"
     gene            5092..5376
                     /locus_tag="VGP50_04690"
     CDS             5092..5376
                     /locus_tag="VGP50_04690"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEV8026701.1"
                     /translation="MAKATWHDQVLAQSDRFEVVEGNVYFPPEALDQRFFKPSPTTTV
                     CPWKGTAHYYTVVVDGASNPDAAWYYPAPKPAAAKIKDHVAFWHGVSVER"
     gene            5453..6142
                     /locus_tag="VGP50_04695"
     CDS             5453..6142
                     /locus_tag="VGP50_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV8026702.1"
                     /translation="MAGGRRILIVDDDMALRHSLAEQLQLHEEFAVVEAGSGGQALDI
                     TKQQNFDAILLDIGLPDMDGRELCRLMRRSGVRAPVLMLTGADSDADAILGLDSGAND
                     YITKPFRLNVLLARLRAQLRQHELSEDAVFTIGPYTFRPSAKLLVDEYGRKKVRLTEK
                     ETAILKYLYRAGDRIIGRDTLLNEVWGYNAGVTTHTLETHVYRLRQKIERDPQKAEIL
                     VTEPGGYRLVP"
     gene            6260..6496
                     /locus_tag="VGP50_04700"
     CDS             6260..6496
                     /locus_tag="VGP50_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026703.1"
                     /translation="MGKKKISERWRRVASDAALVCVYAGVVLTLFGSFAFEHVTRSAG
                     IQIALVSTAMAVLVISGIWLDRREARREGALGEE"
     gene            complement(6514..7464)
                     /locus_tag="VGP50_04705"
     CDS             complement(6514..7464)
                     /locus_tag="VGP50_04705"
                     /inference="COORDINATES: protein motif:HMM:NF021454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HEV8026704.1"
                     /translation="MPSYHYHFAAFALLCLGLPLPAAAQAEQPAAAQTEQPEAAPADQ
                     PEQPRQVFQSLVQQGGRITLQAGIAELSVGSDFGYLGPKDAGTFLVQLWGNPPEYGAD
                     TLGVLLPKDVDALGVDSWAVVIDYDESGYVSDSDANSIDYDDLLKDMQKELADGNHAR
                     TDQGYDPIALVGWAAPPRYDPATHKLYWAKRLRFGDSKTDTLNYNIRVLGRRGVLDLN
                     IIASIDQLPMIEQRVPEILGMVDFRQGNTYAEYDSHVDKVAAYGIAGLIAGGVLAKVG
                     FFKALLFGILAFKKVAIVATLAVFGAISAFFKRLFDKRPG"
     gene            complement(7539..7949)
                     /locus_tag="VGP50_04710"
     CDS             complement(7539..7949)
                     /locus_tag="VGP50_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin family protein"
                     /protein_id="HEV8026705.1"
                     /translation="MTPEQKRLVQESFARVLPIKDQAASLFYDRLFVLDPSLRALFKG
                     DIKRQGALLMAMINTAVTGLNRLDEIVPAVQQLGRRHADYGVRREHYATVASALLWTL
                     EQGLGEAWTPQLKEAWTACYGLLADVMQKAAAAA"
     gene            complement(8035..8436)
                     /locus_tag="VGP50_04715"
     CDS             complement(8035..8436)
                     /locus_tag="VGP50_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026706.1"
                     /translation="MNARSGKILVFESLFYAAYALRLVLLPFHARIYNMPLDLQVFLN
                     ALWLVVLLCYMVWLVARRDWKWPICVFGLFFLLGLPSNLQEIAEFGRTASVLTLSTAA
                     LTLLQVVMQLGGLLFLFWDMATRQWRALPFP"
     gene            complement(8541..9338)
                     /locus_tag="VGP50_04720"
     CDS             complement(8541..9338)
                     /locus_tag="VGP50_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV8026707.1"
                     /translation="MALKLATWNINSVRHRLDLVRRFIAAAQPDVLCLQETRAPDEHF
                     PREAFAALGYTHMLVHGMKGYNGVALLSRLTLSETGTQSWCARTDCRHAIASLPGGIE
                     LHNLYIPAGGDIADPAVNPKFAHKLQFLDEVTTWFAGQRQSLKPMVLVGDLNVAPLET
                     DVWSHKQLLDVVSHTPVEVARLKRLGEAAGFVDAVRHFIPPEQRLYSWWSYRARDWTA
                     SDRGRRLDHIWVTPPLMGALMSAEVFKAARGWPGASDHVPVIVTIGK"
     gene            complement(9343..10026)
                     /locus_tag="VGP50_04725"
     CDS             complement(9343..10026)
                     /locus_tag="VGP50_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV8026708.1"
                     /translation="MTYAIPAPPVAAVAVVGGESFPVHRIYCVGRNYAEHAREMGHDP
                     DREPPFFFMKPADAVVASGSTIPYPPKTKDYQHEIELVVALSKGGRDVPVDKALDLVF
                     GYAVGLDMTRRDLQGEAKKLGRPWDLGKGFDQSAPCAPVSPVGRAGHIGKGAIWLKVN
                     GKTRQQSDISQLIWTVPETISYLSGFVTLAAGDLIYSGTPAGVGAVVKGDRLEGHVDG
                     LEDLVITIG"
     gene            complement(10104..11147)
                     /gene="gtdA"
                     /locus_tag="VGP50_04730"
     CDS             complement(10104..11147)
                     /gene="gtdA"
                     /locus_tag="VGP50_04730"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HEV8026709.1"
                     /translation="MDEAPARTPERETFYRELAPHNMAPLWEVLRALVTREPQTPCVP
                     ALWRYDAVRPYLMRAGALITAKEAERRVLILENPALAGKASITHSLYAGLQLILPGEV
                     AAAHRHTQSALRFVLEGEGAFTAVDGERTYMKPGDFVITPSWTWHDHGNDSDQPMVWL
                     DGLDIPLVALLDASFMEPGNAEQQMVTRPEGDSLARFGAGLLPVDWRPTTRTSPVFNY
                     PYERTRDALETLKRGAEWDACQGLKLRYVNPATGDHAMPTMATFMQLLPKGFAGASYR
                     STDGTVYVCVEGRGETRIAGESFAWGPRDVFVVPSWARHAHLADQESVLFSFSDRPVQ
                     EKLGLWREERAAH"
     gene            complement(11214..>11680)
                     /locus_tag="VGP50_04735"
     CDS             complement(11214..>11680)
                     /locus_tag="VGP50_04735"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8026710.1"
                     /translation="NIVSTAAFYGNYGQAADASAKTALLGLTRAAALEMQRTGVTANA
                     VAPFAATRVTQSMKPANAAQAAYQERALRVPAEPVAALVAWLAGPEAGDVTGQLFGVR
                     GREIFLFSQPRPLARALSGAPDELGAIIATELAPHFLPLETDGDAFGGEPIL"
BASE COUNT         1884 a         3921 c         4025 g         1850 t
ORIGIN      
        1 accggccgtc agcacccatg ccccgacctg gccggagcga tagacgagcg ccgacagcgc
       61 cagcacggag agcgcgtcgg tctccatgtc gaagcgcgcg ccgaagacgc tggcgaggtg
      121 gctgcgccgg gccagccagc cgtcgacgcc gtcgagcgcc aaagcggcag cgcccgccgc
      181 aaccagagcc cagcggccgg tctcgctcag gctggccgtg ccgtcgctga gaatgccgac
      241 gagcagcgcc gcataggcgg cgcgcgtcag ggtgacgaca ttggccgctc cgaagcggcg
      301 atagggatga tagccgaggc cggccacgac gccggccgtg atgagcccat aagcgacgag
      361 caccaccccg acgccgcgcc aagcgagccc gcccgtgacc gcgagcgtca ctgccacgac
      421 ggcgagcaca ccggcaagca gcgccaaccg ccttccggca tcaatcagca gagggcgcgg
      481 cgacacggtg gaaagtcctc ggataaggtt tttgcggcgc cggcctcgcc acggacgggt
      541 ggacgccgga ccagacaatg acccgaaatc gcaacaatca caagccacaa accagtaccg
      601 gacaggacca ggatgcgtca ctcaagggtg tgggatgtcg caaatgcgat tcaaatacgc
      661 gtgatcggat gctatggctg atgtgaaaca gcgaggcgcg cgcatcccgg cacgggccgc
      721 cgcttattcc gccggcgcaa cgagccaaaa tctccctgct gcttttgcgc gcgctgcgat
      781 ttgctcatgg ccaagggacc ggcgctgata gaatgcaggg gatggacgat attcctgccg
      841 ctctgcccga cggtctcgag ggcgccatcg cgaccgacgg gtttgccttt ctcgacggcg
      901 cgacgatgcg cgcgctcctg gagaaggccg gcccgctcgg cgatcgggag cgttttgcga
      961 cgagctggga cgacatgccc gtcgacgcct acatggccga cgggggccgc taccggcggc
     1021 ggcggcatgc cgtctacgcc gtcaccgccg atggcgccat cctgcgccaa tcgcatcagc
     1081 cgcattatca gagccgcgac tacaatccac tccatggcgg cgtcgagcgc tggttcgcgc
     1141 cgatcgagcc ggcaatcggc gacgacccct gtcttttgac gatcctcggc ttctgccgcg
     1201 gcttgttcgg ccgcctcgcg ccggaggttc gcgcgtggcg cgtcgagctc caccagttcc
     1261 gcatcgaagc gcggccgggc gaggccggcc atccgacacc cgagggtatg catcgcgacg
     1321 gcgtcgacta tgtgctggtg ctgctgattc gccgccgcaa catcaggcgc ggaacgacaa
     1381 cgatccacag cctcgagggc aagctgctcg gcagcttcac cctcgccgcg cccttcgacg
     1441 cggcgctggt gcatgatgcg cgcgtctatc acggcgtgac accggtcgag cccgtcgatc
     1501 cggcgctgcc cgcctttcgc gacgtcctcg tcgtgacctt ccgccgcact taggcaacgg
     1561 ccccaccgtg ctcccgccgc gcgggagagc gacccgggct tccacgataa accgcggaaa
     1621 ctcccatcgt cgccgcgatg aactgttgac ggcgcgcctt gcccggtcgg ctatagtgga
     1681 agcgacgcat ccgtatgagg ttcggggtct tgtcgcgacc actttcccct ggcgcatccc
     1741 gatgagcggg gtgccggacg acgcggagct gtggcagcgt gttctggcgc tgcgcggcgc
     1801 cggcgcgacg acagtgctga ccgaggcgca gcgccagccc ggcgccggcc tggcggcgct
     1861 cttgccgctc tacctgccct tggtgcagtg gccgcgcgat cgctgcggcg ccgtcgccca
     1921 tcttggccaa agcctcgacg gccgcattgc cacggcagcc ggggcgtcgc gctgggtgac
     1981 gggagaggcc gacttgctgc atacccatcg catgcgcgcg ctcgccgacg cggtcgtggt
     2041 gggcgccggt acggtgcgtc acgacgatcc tcagctcacc gtgcgacgct gcctcggcgc
     2101 gcagcccgtg cgcgtcgtga tcgacagcga gcgccgcctc ggcgcggatt accggatatt
     2161 ccaggacggc gccgcgccga ccttggtcct ggccgcggcg gaccgcgtgc ggcccggcga
     2221 gcgcttgggg cgggccgaga tcctgccgct gccgcgcggc gccgatggcg ggcttgcgcc
     2281 cggcgaaatc cggcgcgtgc ttgcggcacg cggcctcgct cttctcttta tcgagggcgg
     2341 cggcatcacc atctcgcgct tcctcatggc cggcgcgctc gaccggcttc agctcaccgt
     2401 ggcgccggtc atcctgggct cgggccggcc cagcttgtcg cttcccgaga tcgccgagcc
     2461 ctgccagggg ctgcggccgc ggctgcgccg cttcagcctc ggcgaggaca tgctttacga
     2521 gtgcatcttc cgtgactgag cgaggcgaag gccgcgcctt ctggatcagc gcgcccggcc
     2581 gcggcgagct gcgctccgcc atcctgccgg cgctcgggcc gggcgatgtg ctggtgcgcg
     2641 cgctggcgag cgggatcagc cgcgggaccg aagccctcgt cttcggcggt catgtccccc
     2701 cgagccagta cgtggcgatg cgctgcccgt tccaagaggg cgattttccg gcgcccgtga
     2761 aatatggcta cgcctcggtc ggtgtcgtcg aagcgggccc gggcgcgctg cttggccggc
     2821 gcgtcttctg cctccatccg catcaggatc gctatgtcgt ttccgccgat gccgtgctcg
     2881 cggtgccgga agcggtgccc gacgagcgcg ccgtgctcgc ggccaacatg gagacggccg
     2941 tcaacggcct atgggacgcg gcgccgcttc tcggcgacca catcgcggtc gtgggcgcgg
     3001 gggtggtggg gtcgctgatg gccgcgctgg ccgcgcgcct tccgggaacg cgtgtcgagc
     3061 tcgtcgacat cgatcagacg agagccgtcc ttgccgccgc gctcggttgc ggttttgcgc
     3121 gtcccgccga cgcgacaggc gacgccgatc tcgtcattca tgcgagcggc agcgccgcag
     3181 gtctcgcgac cgcgctgcgt ctcgccggtt tcgaggcgac ggtacttgag atgagctggt
     3241 acggcgacgc gccggtcacg gcgccgctcg gcgaggcgtt ccatgcaaag cgcctgagcc
     3301 tcagagcctc gcaggtgggc gccgtggcga cggcgcgccg cgcgcgctgg tcgcggcgtc
     3361 aacgcctcgc tctcgccctt gagctgctcg ccgatccggt tttcgaccat ctgctcagcg
     3421 gcagcagcag cttcgccgat ctgccgcgga cgatggcaag actggccgac gatccgcgcg
     3481 gcgcgctctg tcacgtcgtc cgatatggct gagccgaccg tacgcaatat tcccgaatca
     3541 cctggacggc ctgcgaaaaa ggattcttcc atgtacagcc tcgcggtcag cgaccatgtc
     3601 atgatcgccc acagtttcgt gggcgagatc ttcggcccgg cgcaacagct ccatggcgcg
     3661 acctatgcgg tcgagatgga gctgcggcgg gcgcagctcg acagcaatgg cctcgtctgc
     3721 gacatcggcc tggcattggc gatgctcaag gacgtgctcg gcgagatcaa ctaccgaaac
     3781 ctcgacgaca tccccgcctt ccgcggtcgc aacactacga ccgagttcct cgccggcgag
     3841 atcttccgcc gctgcgagga ccgcattgcc ggtggcgcgc tcggccccgg caccgccgga
     3901 gcgctggcct cgatgcgcgt cacgttgcgc gagtcgccgg tggcgtgggc cgcctgcgac
     3961 ggcgcgctcg gccgatgacc atcgtcttca tcgtgccggg accgctggga cagctcaccg
     4021 gcggctacct cttcgacaag cgcgtcatcg agggactgcg ggccgccggc cgcgccgtcg
     4081 atgttcgcga gctgcccggc gctttcttcc cgtcagccga cgccgccgcg ctcgccgccg
     4141 ccgacgccgt gctttcggcc ctcccggagg aggatgcggc gatcatcgac gggctggcgc
     4201 tccccgcctt cgcgcaggtg ctgacgccca cgtcgaggcg ccggctgatc ggcttcgtcc
     4261 atcatccgct ggcgcgcgag accggccttg ccgaggacgc gcgcgaaacg ctcgccgggc
     4321 tcgaggcgcg gttgctcacc cggctcaagg gcgtgttgtg cccgagcgcc ggcaccgcct
     4381 gggcgctcga ggagtatggt gtcgcgcgcg agcggatcgc catcgcgccg cccggcaccg
     4441 agaagccggc ggagccggcg cgacgctcgc cgcgcgccgg accgctgcgg ctcctggccg
     4501 tcggcacgat ttcgccgcgc aaaggccatc tgctgctggt cgaagcgctg gcggaggtgg
     4561 cggaccggcc gtgggagctg agctgcatcg gcagcctgac gcgcgacccg gcgacgctcg
     4621 ccgccctcga gcgcgccatc ggccgccacg gcttcgggga acgcgtgaag cttctgggcg
     4681 agcgtccgcc cgagcagctc gccgaagcct atgccgcggc ggatgtcttc gtgctgccgt
     4741 cctatcacga aggctacggg atggccttcg ccgaggcgct cgcccatgga ctgccggtgg
     4801 tcgccaccag cgcggtggcc gagacggtgc cggccgaagc cgcgctgctg gtgccgccgg
     4861 gggatgtcgc ggcgctggcc gcggcgcttc gcctcctcat ggacaatgcc ccgctccggg
     4921 cgcggctggc catgggcgcc gccaaggccg gggcggcgct gcccgattgg cagacggcga
     4981 tcgaccgctg gatcgcggcc ttcgaccggc tgtcggcctg agccccgcgc cctcgcccgt
     5041 ctgcggggaa tcgcctaaga tgaggctcag atcggcggcg gaggttcggg aatggcgaag
     5101 gccacatggc atgaccaggt gttggcgcag agcgaccgct tcgaggtggt cgagggcaat
     5161 gtctatttcc cgcccgaagc gctcgatcag cgcttcttca agcccagccc cacgaccacg
     5221 gtttgtccct ggaagggcac ggcgcattac tacaccgtgg tcgtggacgg ggcctcaaat
     5281 cccgatgcgg catggtacta tccggcgccg aagccggcgg cagcgaagat caaggaccat
     5341 gtcgccttct ggcatggcgt cagtgtcgag cgctgaggcg gggactgcgg ccacagcatc
     5401 gcccctataa cgaagcacaa gaggcatccg ccgcacgggc ggaggaggaa aaatggcggg
     5461 cggtcgcagg atcctgatcg tcgacgatga catggcgttg cggcattcgc tggccgagca
     5521 gctgcagctc catgaggagt tcgccgtggt cgaggccggc tccggcgggc aggcgctcga
     5581 catcaccaag cagcagaatt tcgatgccat cctgctcgat atcggcctcc cggacatgga
     5641 tggccgcgag ctctgccggc tgatgcggcg ctccggggtg cgcgcgcccg tgctgatgct
     5701 gaccggcgcc gacagcgatg ccgacgccat tctcggcctc gacagcggcg ccaacgacta
     5761 catcaccaag ccgtttcgcc tcaacgtgct gctggcgcgc ctgcgcgcgc agctgcgcca
     5821 gcacgagctg agcgaggatg ccgtcttcac catcggtccc tacaccttcc gcccgagcgc
     5881 caagctgctg gtcgacgagt atgggcgcaa gaaagtgcga ctcaccgaga aggagacggc
     5941 gatcctcaaa tacctctatc gcgccggcga ccggatcatc ggccgcgata cgttgttgaa
     6001 cgaggtgtgg ggatacaacg ccggcgtcac cacccatacc ctcgagacgc atgtctatcg
     6061 gctgcgccag aaaatcgagc gcgacccgca gaaggcggag atcctcgtca ccgagcccgg
     6121 cggctaccgg ctggtgcctt agccggcgag gctgctgcat caaagtgtcg caggccgctc
     6181 aacctgcgcg tcaatctctt ggaaagaact cgcgccttat tgaaggacct cgcacacgta
     6241 ctttgagccg ggggccacaa tggggaagaa gaagatcagc gagcgctggc ggcgcgtcgc
     6301 aagcgatgcg gccctcgtct gcgtctatgc gggcgtcgtg ctgacgctct tcggcagctt
     6361 cgccttcgag catgtgacgc ggagcgccgg catccagatt gcgctcgtct ccacggccat
     6421 ggcggtgctg gtgatatccg gcatctggct cgatcggcgc gaagcccgcc gcgaaggcgc
     6481 cctcggcgaa gagtagccgc cgccggcggc cgttcagccc ggccgtttgt cgaacaaccg
     6541 cttgaagaaa gcactgatcg cgccgaagac ggcgagcgtc gcgacgatcg ccaccttctt
     6601 gaaggcgaga atgccgaaaa gcagcgcctt gaagaagccg accttggcga gaacgccgcc
     6661 cgcgatcaac ccggcaatgc cgtaagccgc gaccttgtcg acatgcgagt cgtattcggc
     6721 ataggtgttc ccctgcctga agtccaccat gccgaggatc tccggcacgc gctgctcgat
     6781 catcggcaac tgatcgatcg aggcgatgat gttgaggtcg aggacgccgc ggcggccgag
     6841 cacgcggatg ttgtagttca gggtatcggt cttgctgtcg ccgaaacgca ggcgcttcgc
     6901 ccaatagagc ttgtgcgtcg ccggatcata gcgcggcggc gccgcccagc cgacgagcgc
     6961 gatcgggtcg tagccctgat cggtgcgcgc gtggttgccg tcggcaagct ccttctgcat
     7021 gtccttgagc aggtcgtcgt agtcgatgct attggcatcg ctgtcggaga cgtagccgct
     7081 ttcgtcgtag tcgatgacga cggcccaact atcgacgccc agcgcgtcga cgtctttggg
     7141 caacagcacg cccagcgtgt cggcgccgta ttcgggcggg ttgccccaaa gctgcacgag
     7201 aaacgtcccg gcgtccttgg gtcccaaata gccgaaatcc gacccgacgc tgagctcggc
     7261 gatgccggcc tggagcgtga tccggccgcc ttgttggaca aggctctgga atacctgccg
     7321 cggttgctcc ggctggtccg ccggtgctgc ctccggctgc tcggtctgcg ctgccgcggg
     7381 ctgctcggcc tgtgctgccg ccggcagcgg caggcccagg cataggaggg cgaaggcggc
     7441 aaaatggtaa tggtacgacg gcatagctcg ctccccgtca cccgacgacg cgggccagca
     7501 gagcgcagtc cccgttaaga cgcgatgaac gcctgggatc aggccgccgc ggccgccttc
     7561 tgcatgacgt cagccagcaa tccgtaacag gcggtccatg cttctttcag ctgcggggtc
     7621 caggcctcac ccaaaccttg ctcgagcgtc cacagcaggg cgctggccac ggtcgcgtag
     7681 tgctcgcggc gaacgccgta atcggcatga cggcggccca gctgctgcac ggcgggcacg
     7741 atctcgtcga gccggttgag gccggtgacc gcggtgttga tcattgccat cagcaacgcg
     7801 ccctggcgct tgatgtcgcc cttgaacagg gcccgcaagc tcggatcgag cacgaagagc
     7861 cggtcgtaga aaaggctggc tgcctgatcc ttgatgggca gcacgcgtgc gaagctctct
     7921 tggacgagcc gtttctgttc cggtgtcatg gccacgctcc ctgaatgtgt cggcggctaa
     7981 ttatgcaact gcccgtttat tgcgcaacgt tgaatattgg gatgcttcgg tcgactatgg
     8041 aaaagggagg gcgcgccatt gccgggtcgc catgtcccag aagaggaaga gcaagccgcc
     8101 gagctgcatc accacctgaa gcagcgtcaa tgccgcggtc gagagcgtaa gaacggaggc
     8161 agtgcggccg aactcggcga tctcctggag gttggatggc agccccaaga ggaagaaaag
     8221 gccgaaaacg cagatcggcc acttccagtc gcgccgggcg acgagccaaa ccatgtagca
     8281 gagaagaacc accagccaca gtgcgttgag gaacacctgg aggtccagcg gcatgttgta
     8341 gatcctggcg tgaaacggca gcaggaccag cctcaatgcg taggcggcgt agaagagcga
     8401 ctcgaacacg aggatctttc ccgaccgagc attcacggcg atctccccat ctccggcgaa
     8461 cgagccgagg ctaggggcgg ccgtgttaag gggagattga actggcgtcg cttcgcctgc
     8521 gaaggccacc accaccgaga ctatttgccg atcgtgacga tgaccggcac gtgatccgat
     8581 gcgccgggcc agccgcgggc ggccttgaag acctcggcgc tcatgagcgc gcccatcagc
     8641 ggcggcgtca cccagatgtg gtcgaggcgc cggccgcgat cggaggccgt ccagtcgcgg
     8701 gcgcgatagc tccaccagct gtagagccgc tgttcgggcg ggatgaaatg gcgcacggcg
     8761 tcgacgaagc cggccgcctc gcccagccgc ttcagccgcg ccacctcgac gggggtgtgg
     8821 ctcaccacgt cgaggagctg cttgtgcgac cagacatcgg tctcgagcgg cgccacgttg
     8881 agatcgccca cgagaaccat gggcttgagc gactggcgct gcccggcgaa ccaggtcgtg
     8941 acctcgtcga ggaactgaag cttatgggcg aatttcggat tgacggcagg atcggcaata
     9001 tcgccgccgg cggggatgta gagattgtgg agctcgatcc cgccggggag gctcgcaatc
     9061 gcatggcggc aatcggtgcg cgcgcaccag ctctgggtcc cggtttccga cagcgtcagg
     9121 cgcgagagaa gggcaacgcc gttatagccc ttcatgccgt gaaccagcat gtgggtgtag
     9181 ccgagcgccg cgaaggcctc gcgcgggaag tgctcgtcgg gcgccctggt ctcttgaagg
     9241 cagaggacgt ccggctgcgc cgcggcgatg aagcggcgga cgaggtcgag ccggtgccgc
     9301 accgagttga tgttccaggt cgcaagctta agcgccatag gctcagccga tggtgatgac
     9361 gaggtcctcg aggccgtcga catgaccctc gaggcggtcg cctttgacga cggcgccgac
     9421 cccggctggt gtgcccgagt aaatgagatc tcctgccgcc agcgtaacga agcccgaaag
     9481 ataagagatc gtttcgggga cggtccaaat aagttgcgag atatcgctct gctgccgggt
     9541 cttgccgttc actttgagcc agatcgcgcc cttgccgata tggcccgctc ggcctaccgg
     9601 cgagaccggg gcgcacgggg ccgactggtc gaagcccttg ccgaggtccc agggacggcc
     9661 gagcttcttg gcctcgccct ggaggtcgcg ccgcgtcatg tcgagcccga cggcatagcc
     9721 gaagacgagg tcgagtgcct tgtcgaccgg cacgtcgcgc ccgcctttgg acagcgcgac
     9781 caccagctcg atctcgtgct gatagtcctt ggtcttgggc ggatagggga tggtgctgcc
     9841 gctggcgacg acggcgtcgg ccggcttcat gaagaagaaa ggcggctcgc ggtcggggtc
     9901 atggcccatc tcgcgcgcat gctcggcata gttccggccg acgcaataga tgcgatggac
     9961 gggaaagctc tcgccaccaa ccaccgcaac ggcggcgacg ggtggcgcag ggatcgcgta
    10021 ggtcatcaaa actcccgaag tgaagctggc ggcgcgcctg gcggtatatc aatcatccgg
    10081 ttctttggat accggggccg tcgctaatgc gcggcgcgct cctcacgcca gagcccgagc
    10141 ttctcctgca cggggcggtc ggagaagctg aagagcaccg attcctggtc ggccagatga
    10201 gcgtggcgcg cccagctcgg cacgacgaag acgtcgcgcg gcccccaggc gaagctctcg
    10261 ccggcgatgc gcgtctcacc acgcccttcg acgcagacat agacggtgcc gtcggtcgag
    10321 cgataggagg cgccggcaaa gcccttgggc agaagctgca tgaaggtcgc catggtgggc
    10381 atggcatggt cgccggtcgc agggttgacg tagcgcagct tcaagccctg gcacgcatcc
    10441 cattcggcgc cgcgcttcaa tgtctcgagc gcgtcgcggg tgcgttcgta ggggtagttg
    10501 aagacgggcg aggtccgggt cgtcggccgc caatccaccg gcaagagccc ggcgccgaag
    10561 cgggcgagcg aatcgccctc gggccgggtc accatctgct gctcggcatt gcccggctcc
    10621 atgaagctcg catcgagcag cgccaccagc ggaatgtcga ggccgtcgag ccacaccatg
    10681 ggctggtcgc tgtcgttgcc gtgatcatgc caggtccagg acggcgtaat gacgaagtcg
    10741 cccggcttca tgtaggtgcg ctcgccgtcg acggcggtga aggcgccttc gccttcgagg
    10801 acgaagcgca gcgccgactg cgtgtggcga tgcgcggccg ccacttcgcc gggcagaatg
    10861 agctgcagcc ccgcatagag cgaatgcgtg atcgaggctt tcccggcgag cgccgggttc
    10921 tcgaggatga gcacgcgccg ctcggcctct ttggcggtaa tgagagcgcc ggcgcgcatc
    10981 agatagggcc gcaccgcgtc gtagcgccag agcgccggca cgcaaggcgt ctgcggctcg
    11041 cgggtgacga gcgcgcgcaa cacctcccag agcggcgcca tgttgtgcgg cgcgagttcg
    11101 cgatagaacg tctcgcgctc gggcgttcgg gccggggcct catccatcgc gcacctcgtc
    11161 gcgcttatcg acgaccgctc catctatgac aaatccatgc ggccaccgcc acctcagagg
    11221 atgggctcgc ccccgaaggc atcgccgtcg gtctcgagcg gcagaaaatg cggcgccagc
    11281 tctgtggcga tgatcgcgcc aagttcatcg ggcgcgccgc tcagcgcgcg cgccaggggg
    11341 cgcggctggg agaagaggaa gatctcgcgg ccgcgcacgc cgaagagctg gccggtgaca
    11401 tcgccggctt cgggcccggc aagccaggcg accaacgcgg cgacgggctc ggcgggcacg
    11461 cggagcgcgc gctcctggta agccgcctgc gccgcgttcg cgggtttcat cgactgcgtg
    11521 acgcgcgtcg ccgcgaaagg cgcaacggcg ttcgccgtga cgccggtccg ttgcatctcg
    11581 agcgccgcgg cgcgcgtcag cccgaggagc gcggtcttgg cgctcgcatc ggcggcctgt
    11641 ccgtaattgc catagaaggc ggcggtcgag acgatgttga
//
LOCUS       DASYNS010000150         6734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1595187, whole genome shotgun sequence.
ACCESSION   DASYNS010000150 DASYNS010000000
VERSION     DASYNS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595187"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..811)
                     /locus_tag="VGP50_04740"
     CDS             complement(113..811)
                     /locus_tag="VGP50_04740"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEV8026711.1"
                     /translation="MTITPTSATTPAAAGASASNPTGVLGFTQNFNTFLTLLTTQLQN
                     QDPLSPLDSNQFTQQLVMFSQVEQQIQTNSQLTTLIAGQAASETVAALPMVGQTIEYS
                     GNQAVLQNGQAAYSYTLPSAAASASIAIQDSGGNTVFQTTTASPSAGANSFVWNGQTT
                     AGQQLPDGGIYTIQVSATAANNTAVTATTAAVGTITGVSVINNAATFNVSGLAVPMTQ
                     LLSIITNSPSSASN"
     gene            complement(824..2140)
                     /locus_tag="VGP50_04745"
     CDS             complement(824..2140)
                     /locus_tag="VGP50_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEV8026712.1"
                     /translation="MARRLLPSIRMNTQAIASLPPDIVPAPPRATEAANAVSFGAMLA
                     PTNAPSIPSETPHDIAGSAKAAAARAQRAVSDKKSSNDNPPASAAAIAPQPLQQAASQ
                     AGATTSAAAAAATASSASTAPGLLAGTLLAGASSGVSGAATGPATTLANSIAAPPASG
                     TSTGSSNTVQFGAMAAALDARIAAGAPGLASQPRVALADLAASQDKLGAADANHSALS
                     LEALQGVAKSPTPADAAMDEKLLADAAKDAAAAATTSLEPAATGSASDQPPADASTSI
                     AADAASVVAPPPPPPLAAAPAPPSPTTPSAAAQVALALPQAFKSGDNHIQIQLQPAEL
                     GAIDVKLNVNHDGRVTMVVSADRSDTLNLLRQDSSSLTQALRDAGLQADSSSLSFNLR
                     GGFNQQASNGGAAAGGTASSSAEPDDIAVPAGALQRRHAGSLDIHV"
     gene            2395..2703
                     /locus_tag="VGP50_04750"
     CDS             2395..2703
                     /locus_tag="VGP50_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026713.1"
                     /translation="MALIETSSIAPLVRTPSTERQTPEPTPAPAAAAANAPAPAPSAV
                     GFTLTFDPDTQRMFLEARDPATGFVIDRMPAKYVATQFSAQSGVNAAASRGTRIDDAS
                     "
     gene            2925..3812
                     /locus_tag="VGP50_04755"
     CDS             2925..3812
                     /locus_tag="VGP50_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HEV8026714.1"
                     /translation="MSSNIILGASTQQNLLSLQQINNQMDTAQNRLSTGLKVSSAVDD
                     AVSFFQAQSLQNRASDITTRNSAIDQGVSTLTTATQAVQSVVALLQQLQGITTTAKTE
                     TAAQRAASATQFDALAKQLNNLVNDASYQGLNLVNSTASVLKLQFSQVSTSTLKVAGY
                     NLLLSKVLTSKAGGLSKVAIVAASKLASILASGTFSTVSNKTSKFDNAYNLLQNVVYA
                     AQSDAQALGGNVSFLQTRLAFGQQYATTLTGGASKLTVADVNLESTNLVTLQTRQSLS
                     IQSLSIATQSEQSVLRLFH"
     gene            3920..4342
                     /locus_tag="VGP50_04760"
     CDS             3920..4342
                     /locus_tag="VGP50_04760"
                     /inference="COORDINATES: protein motif:HMM:NF019024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HEV8026715.1"
                     /translation="MPLTINLKPGEKLIVNGVVIQNAHHVAKLLVHNEAALLREKDIL
                     AEDAANTPARRIYFAIQCQYLFPDKREHYLPLVQRFLGEFADAAPSTLPLVHKAGQEV
                     ADGHLYQALKTAKQLIAREQEIFNGCTTTSLRTDAASR"
     gene            4296..4724
                     /locus_tag="VGP50_04765"
     CDS             4296..4724
                     /locus_tag="VGP50_04765"
                     /inference="COORDINATES: protein motif:HMM:NF018959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HEV8026716.1"
                     /translation="MAAPQQAYARTPLPGNPARTEAWALLEAARLMAVAKDKGREEIL
                     AAVRKNWRLWTIFQVSLIDADCKLPDAVRANLLGLANFIDRHVGQILADPAPKHLDVL
                     IHINRQIGEGLLEGMRAGAGASAAQNAPTAEPPKSLRESA"
     gene            4729..6594
                     /locus_tag="VGP50_04770"
     CDS             4729..6594
                     /locus_tag="VGP50_04770"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8026717.1"
                     /translation="MQDADLPSVDELVSEAARDFAQGDIEAAIATLSAASARDPDHMP
                     VVFLAALLAWRLGDAAKALMLARRCFDKASMNGTVAEIVASLYAQTGDLTESLCYGKL
                     AIALPVDATLAAWLPPNFPTFDAAFLTIQKTPLLAQARRLLAAGKLEEALNKARQHVE
                     VSPDDWNGRLFYGTTLLRAGRDASALEILSPLTDAAEPVPPAASALARACAAVGEALS
                     AQQWHDAAVRGAPDDASVAAARIADAPWLNVDSVQHAAWRAAWRTRFVQPPKPRQRRW
                     SDEKLVIGYVVSAFADRQDAAAVAAVARAHARPGTTVIGYGLGAQSWNENVLLRGAFD
                     NWRDVSGLDPATLAKTVAGDGVDVVIDVGGAASAVNLQALARINTALRVAWLADPAGL
                     ETAIYDAAIAPPAHAAIAAWPAPAGGYPLLREWTRAREHSPAEAHCRFGADVRLAQID
                     ERTRRLWCAILEAAPTATLLLRANDMSMPVNINRLVERFGRRLAARIDVVDAPAAESF
                     YRKVDLALAPAVAVSARVVGEALACGVPLLALDDGGLWQPYSRMLRDLGLAELVAADA
                     DAYVAQATALAGSPDRRAAQAARVAAVADRGGSVAGAIASAIETAARTALRRVAA"
BASE COUNT         1044 a         2259 c         2313 g         1118 t
ORIGIN      
        1 gtattggagt tggcgatgtc gttcgagatc atgtcgatcg cctgggtatt ggaagaaaga
       61 cccgagacgg ccgagaatag tgcgccgttg atggtcatgg ggtatctcct aattagttgc
      121 tcgccgacga cggcgaattc gtgatgatgc tcagcagctg ggtcatcggc accgcgaggc
      181 cggagacgtt gaaggtggcg gcattgttga tgacgctgac gccggtgatg gtgccgacgg
      241 cggcagtggt cgccgtgacc gccgtgttgt tggcggcggt ggcgctcacc tggatcgtgt
      301 agattccgcc gtccggcagc tgctggcccg cggtggtctg gccgttccac acgaaactgt
      361 tggcgccggc gctggggctg gcggtcgtcg tctggaagac ggtgttgccg cccgagtcct
      421 gaatggcgat gctggcggag gcggcggcgc tcggcagcgt ataggagtag gcggcctggc
      481 cgttctgcag caccgcctga ttgccgctgt attcgatggt ttgcccgacc atgggcagcg
      541 cggcgacggt ttcgctcgcg gcctggccgg cgatcaacgt cgtgagctgg ctgttggttt
      601 ggatctgctg ctcgacctgg ctgaacatca cgagctgctg cgtgaactga ttcgaatcga
      661 gcggcgacag cggatcctga ttctggagct gcgtggtgag cagcgtcagg aacgtgttga
      721 agttctgggt gaagccgaga acgccggtcg ggttgctggc gctggcgccg gctgctgccg
      781 gtgtcgtggc gctggtgggt gtgatcgtca tgggatcggt tccttagacg tgaatgtcga
      841 gggagcccgc gtggcggcgc tggagcgccc ccgccggcac ggcgatgtcg tccggctcgg
      901 cggatgacga ggcggttccg cccgccgcgg cgccgccgtt ggaggcctgc tgattgaagc
      961 cgccgcgcag attgaagctc aagctgctgc tgtccgcctg caggccggcg tcgcgcaagg
     1021 cttgcgtgag cgagctcgaa tcctgtcgca gcagattgag cgtgtcgctg cgatcggccg
     1081 acacgaccat cgtgacgcga ccgtcgtggt tgacgttgag cttgacgtcg atggcgccga
     1141 gctccgccgg ctgaagctgg atctggatgt ggttgtcgcc gctcttgaag gcctgaggca
     1201 aagcgagcgc gacttgcgcg gcggcgctcg gcgtggtcgg cgacggcggt gcaggagcgg
     1261 cggcgagcgg cggcgggggc ggcggcgcaa cgaccgacgc cgcatccgca gcgatcgacg
     1321 tgctcgcgtc ggcaggcggc tggtccgaag cactcccggt cgcagcaggc tcgagcgacg
     1381 tcgtcgccgc ggcggcggcg tcttttgccg catctgcaag cagcttctcg tccatcgccg
     1441 cgtcggccgg cgtcggcgac ttcgccactc cctggagcgc ttcgagcgac agcgcggagt
     1501 gattggcgtc cgccgccccg agcttgtcct gcgacgccgc gagatcggcg agtgccacac
     1561 gcggctgcga cgccaagccc ggcgctccgg cggcgatgcg agcgtcgagc gcggccgcca
     1621 ttgcgccgaa ctgcacggtg ttggaggagc cggtcgacgt gccggaagcg ggcggcgcgg
     1681 cgatggaatt cgcgagggtg gtcgcgggac ctgtggccgc gcccgagaca ccggagctcg
     1741 ctcccgcgag cagcgtgcca gcgagaagac ccggcgcggt gctcgccgac gacgcggtcg
     1801 cggcagcggc tgcggcagat gtcgtcgcgc cggcctgact cgcggcttgc tgcagcggtt
     1861 gcggcgcgat cgccgcagcc gacgcaggag gattgtcgtt gctcgatttc ttgtcgctca
     1921 ccgcgcgctg agcgcgagcg gcggcggctt tcgcggaccc ggcaatgtcg tgcggcgttt
     1981 cgctggggat gctgggcgcg ttcgtcggcg cgagcatcgc cccgaaagac acggcgttgg
     2041 cggcttcggt ggcgcggggt ggcgcgggga cgatgtcggg agggagggag gcgatggctt
     2101 gggtgttcat gcggatgctt ggcagcaagc gccgtgccat cgcggcaaca tcttgatatg
     2161 gcgatgactt cgcgcggcgg agcggtccgg ccgcgggacc ggagtgcgcc gtggcggcaa
     2221 ggcgtgccga gtgacatcag gcaatctttg cccgatcgca gcgactttgg cacagagctt
     2281 gcgtcgattc cggcggagtt tcctttggtt gcgacggcag aaatcgcgaa aacatcgctc
     2341 gatttttagt tttcgtcgga tttttgtcta gaacgcggct ccgggagatc gatcatggct
     2401 ttgatagaga cgtcatcgat agcgcccctc gtgcggacgc ccagcaccga gcggcaaacg
     2461 cctgaaccga cgccggcgcc cgccgccgcg gcagcgaacg cgccggcacc ggcgccgagc
     2521 gccgtcggct tcacgctcac cttcgatccc gacacgcagc gcatgttcct cgaggcgcgc
     2581 gaccccgcga ccggcttcgt catcgatcga atgccggcga aatacgtggc gacgcagttc
     2641 tcggcgcagt cgggcgtcaa tgccgcagcg tcgcgcggca cgcgcatcga cgacgcgtcg
     2701 tgaggtcgac ccggtaaaat ctgccgcctc ggcaggtctc gcccggcaag acttgccgcc
     2761 gacccggcga cacacccctt tttcgcgaag agtttctccc cgaaaccacg gatttccgcg
     2821 catttgcgcg gtcgtccgga ggatgacgcc gatggcatgg ccattgcggt gggaagagcg
     2881 ggtcgcaaag cggcctgtgt acttacccca gaaaggatcc actcatgtcc agtaacatca
     2941 tcctcggtgc gtcaacccag cagaacctgc tgtcgttgca gcaaatcaac aatcagatgg
     3001 acacggcgca gaaccgcttg tccaccggcc tcaaggtgtc gagcgccgtc gacgacgcgg
     3061 tcagcttctt ccaggctcag tcgctccaga accgcgcctc cgacatcaca acgcgcaaca
     3121 gcgcgatcga ccagggcgtc tcgaccctga ccaccgccac tcaggcggtg cagagcgtgg
     3181 tggcgctgct ccagcagctc cagggtatca ccaccactgc caagacggag actgccgcac
     3241 agcgcgccgc ttcagccact cagttcgatg ccctggcgaa gcagctgaac aacctcgtca
     3301 acgacgcctc ttaccagggc ctcaacctgg tgaactcgac ggcgtcggtg ctgaagctgc
     3361 agttcagcca ggtctcgact tcaaccctga aggtcgcggg ctataacctg ctcctcagca
     3421 aggtgctgac cagcaaggcg ggcggcctct ccaaggtcgc cattgtggca gcttcgaagc
     3481 tcgcctcgat cctggccagc ggcacgttct cgacggtcag caacaagacc tcgaagttcg
     3541 acaacgccta caatcttctg cagaacgtcg tctacgccgc gcagtcggac gcgcaagcgc
     3601 tcggcggcaa tgtgagcttc ctgcagaccc gcctcgcctt cggtcagcaa tatgccacga
     3661 cgctgaccgg cggcgccagc aagctgacgg ttgctgacgt gaacctggag agcaccaacc
     3721 tcgtcaccct ccagacgcgc cagagcctct cgatccagtc gctgtcgatc gcgacccagt
     3781 cggagcagtc agttcttcgt ctcttccact aagtcgcgtc tctaactctg gcggtgggcg
     3841 gccaacaggc cgcccactcg tccagggaaa attaaccgcg ggcgtctaaa acgctgtgcg
     3901 ggaaccacgg agcagtagaa tgccgctcac catcaacctg aagccgggcg agaagctcat
     3961 cgtcaacggc gtcgtcatcc agaacgctca tcacgtcgcc aaactcctcg tccacaacga
     4021 ggccgcgctg ctgcgcgaga aggacatcct cgccgaggat gccgcgaata cgcccgcacg
     4081 tcgcatctat ttcgcgatcc agtgccagta cctcttcccc gacaaacgcg agcactatct
     4141 gccgctcgtc cagcgcttcc tcggcgagtt cgccgatgcc gcgccgagca cgctgccgct
     4201 cgtccacaaa gccgggcagg aggtggcgga cggccacctc tatcaagcgc tgaagacggc
     4261 aaaacagctc atcgccagag agcaggagat ctttaatggc tgcaccacaa caagcctacg
     4321 cacggacgcc gcttcccggt aatccggcgc ggaccgaggc ctgggcgctg ctcgaagccg
     4381 cgcgcctcat ggccgtggcg aaggacaaag gccgtgagga gatcctcgcc gccgtgcgca
     4441 agaactggcg tctctggacg attttccagg tgagcctgat cgatgccgat tgcaagctgc
     4501 ccgacgcggt gcgtgccaat ctgctgggtc tcgcgaattt catcgaccgc catgtcgggc
     4561 agatcctcgc cgaccccgct cccaagcatc tcgacgtgct gatccatatc aaccgccaga
     4621 tcggcgaggg ccttctcgaa gggatgcgcg ccggcgcggg cgcgagcgcg gcgcagaacg
     4681 ccccgacggc agaaccgccg aagtcactgc gcgagagcgc ctagcgccat gcaggacgcg
     4741 gacctcccct cggtcgacga actcgtgtcc gaggcggcgc gcgatttcgc ccaaggcgat
     4801 atcgaagccg ccatcgccac gctgagcgcg gcgagcgctc gcgatcccga tcacatgccg
     4861 gtcgtcttcc tggcggcgct cctggcgtgg cggctcggcg acgccgccaa ggcgctgatg
     4921 ctggcgcgcc gctgcttcga caaagcgtcc atgaacggca ccgtcgccga gatcgtcgcc
     4981 tcgctctacg cgcagaccgg agacctcacc gagagcctct gctacggcaa gctggcgatc
     5041 gcgctgcccg tcgatgcgac gctcgccgca tggttgccgc cgaatttccc gaccttcgac
     5101 gccgcgtttc tgacgatcca gaagacgccg ctcctcgcgc aggcccggcg gctgctcgct
     5161 gccggcaagc tggaagaggc cttgaacaag gcccgtcagc atgtcgaggt gtcgcccgac
     5221 gattggaacg gcagactctt ctatggcacg accttgctgc gcgccggacg cgacgcgagc
     5281 gcgctcgaga tcctgtcgcc gctgaccgac gctgcggaac cggtgccgcc tgccgccagc
     5341 gcgctggcgc gcgcctgcgc cgcggtgggc gaggcactga gcgcgcagca gtggcacgac
     5401 gccgccgtcc gcggcgcgcc tgatgacgcg tcggtcgcgg ccgcgcgcat cgccgatgcg
     5461 ccctggctca acgtcgattc cgtccaacat gccgcgtggc gcgccgcctg gcgcacgcgc
     5521 ttcgtccagc cgccgaagcc gcggcagcgg cggtggtcgg atgaaaagct cgttatcggg
     5581 tatgtcgtct ccgccttcgc cgaccggcag gatgccgccg cggtcgcggc cgtcgcccgc
     5641 gcccatgcgc ggcctggcac gacggtgatc ggctatggtc tcggcgcgca gagctggaac
     5701 gagaatgtgc tgctgcgcgg cgctttcgac aactggcgcg acgtcagcgg gctcgacccg
     5761 gcgaccttgg ccaagaccgt cgccggcgac ggcgtcgacg tcgtgatcga tgtcggcggc
     5821 gcggcctctg cggtcaatct gcaggctttg gcgcgcatca atacggcgct gcgcgtcgcc
     5881 tggctcgccg atcccgccgg tctcgaaacc gccatctatg acgccgccat cgcgccgccc
     5941 gcccatgccg ccatcgcggc ctggccggcg ccggccggcg gctatccgct gctgcgcgag
     6001 tggacgcgcg cgcgcgagca tagtccggcc gaggctcatt gccggttcgg cgccgatgtg
     6061 cggctcgcgc agatcgacga gcggacgcgg cgcctgtggt gcgccatcct cgaggccgcg
     6121 ccgacggcga ccctgctgct ccgcgccaac gacatgtcga tgcccgtcaa catcaaccgg
     6181 ctggtcgagc gtttcggccg acgccttgcc gcgcgcatcg acgtcgtcga tgcgccggcc
     6241 gcggaaagct tctatcgcaa ggtcgatctc gcgctggcgc ctgccgtcgc cgtgtcggcg
     6301 cgcgtggtgg gcgaagcgct ggcctgcggc gtgccgctgc tcgcgctcga tgacggcggc
     6361 ctgtggcagc cctattcccg catgctgcgc gatctcggtc tcgccgagct cgtcgctgcc
     6421 gatgccgatg cctatgtggc gcaggcgacg gcgctggcgg gctcgcccga ccggcgcgcc
     6481 gctcaggccg cgcgggtcgc ggcggtcgcg gatcgcggag gaagcgtcgc cggcgcgatc
     6541 gcgtcggcga tcgagacggc ggcgcgaacg gcgctccggc gggtggccgc ttgaccgcca
     6601 gcggtctcgc cgcgcggtcg cgcgcggcct ttgccgcgcg ctttcctcgg gtcgtcgcgg
     6661 cgatcgacgc gcaggaaccc caatccaccc tcgtttttga gggcgagatg ccggtcgacc
     6721 tcgtggtcgg cgag
//
LOCUS       DASYNS010000151         3852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4558885, whole genome shotgun sequence.
ACCESSION   DASYNS010000151 DASYNS010000000
VERSION     DASYNS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4558885"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VGP50_04775"
     CDS             <1..1000
                     /locus_tag="VGP50_04775"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEV8026718.1"
                     /translation="AFLPKRQLLPYLEAVLRVYNEFGRRDNLFKSRIKILVQSLGIEK
                     MRDAVEAEFAAMGETGLELEPETVAAIAAHFQPPAYEPPGADDEAVLARAIDADPRFA
                     RWVEQNVAPHRAPGHAIVTVSLKPYGRPPGDCSAEQMELLADLAEAYSKGELRVSYEQ
                     NLVLAHVRRADLAALWRRLDDAGLGTANATLASDIIACPGLDYCSLANARSIPVAERL
                     SAHLAARDHEIGELRIKISGCINACGHHHVGHIGILGVDKNGEEFYQITIGGSADENA
                     ALGAIVGPAVPSERVTDAVDTLIGVYLRHRSDGERFPETYRRLGVAPFKEALYAAH"
     gene            987..1481
                     /locus_tag="VGP50_04780"
     CDS             987..1481
                     /locus_tag="VGP50_04780"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEV8026719.1"
                     /translation="MPLIKNGGFVADPWRHIEDGAPLPADAPATVSWKRWSSEREILV
                     GHNGRLGLRLPNDVPPLSLAQDAPRFDLIALAFPKFIDGRAFSQARLLRSRCGFTGEL
                     RATGDVLRDQLLFMRRCGIDAFEVSERAIAEDWLAALDDFDLFYQPAADGRPWIARQR
                     LRAG"
     gene            1570..2229
                     /locus_tag="VGP50_04785"
     CDS             1570..2229
                     /locus_tag="VGP50_04785"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV8026720.1"
                     /translation="MSLSIGDVAPDFEADTTQGRIRFHDWISDSWAVLFSHPKDFTPV
                     CTTELGYMARLKPDFDKRNTKIIGLSVDAVQDHGRWANDIKETQGFAPNYPIIGDPDL
                     KVAKLYGMLPASASGDAAKRTPADNQTVRTVFVIGPDKKIKLLLVYPMTTGRNFDEVL
                     RVIDSLQLTAKHRVATPVNWKPGDDVIIAGSVSDDEAKKIYPQGWKAPRPYIRIVPQP
                     R"
     gene            complement(2461..2985)
                     /gene="thpR"
                     /locus_tag="VGP50_04790"
     CDS             complement(2461..2985)
                     /gene="thpR"
                     /locus_tag="VGP50_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEV8026721.1"
                     /translation="MLRLFVGIELPPEIKLRLSPLCAGVPGARWVDPENHHVTLRFIG
                     EVDEGTAGDIDAALAQIKARRFEMALAGVGQFGERSLWVGVEKNPALVHLRDKIERAM
                     VREGLPPEGRRYAPHVTLARLKGTSDERLHAYLRQHALFRAQPFTVGHFSLVASYLTK
                     SGAIYEDQADYALG"
     gene            complement(2990..3595)
                     /locus_tag="VGP50_04795"
     CDS             complement(2990..3595)
                     /locus_tag="VGP50_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEV8026722.1"
                     /translation="MLFATLLFPSVAEAGTRVLAFGDSLTAGFGLLPDQAFPVRLQAR
                     LKASGTVVEMTNAGVSGDTSAGGLARLDWALAEHPDVVLVELGANDALRGIDPKQTYA
                     NLDKILARVKASGAKVLLLGMLAPPNWGHDYEKEFDAIYPALARKYDVPLYPFFLDGV
                     ATLPDLNQEDGIHPNKQGVEIIVGRVAPYVQRLLGGKGAQG"
BASE COUNT          636 a         1380 c         1217 g          619 t
ORIGIN      
        1 cgcttttttg ccgaaacgcc agctcctgcc gtatctcgag gcggtgctcc gcgtctacaa
       61 cgagttcggc cggcgcgaca acctcttcaa gtcgcgcatc aagatcctcg ttcagagcct
      121 cggcatcgag aagatgcgcg acgccgtcga ggccgagttc gccgccatgg gcgagaccgg
      181 tctcgagctc gagcccgaga cggtcgcggc catcgccgca catttccagc cgcccgctta
      241 tgagccgccc ggcgcggacg acgaagccgt gctggcgcgc gccatcgacg ccgatccgcg
      301 cttcgcccgc tgggtcgagc agaacgtcgc gccccatcgc gcgcccggcc acgccatcgt
      361 cacggtgtcg ctgaagccct atggacggcc gcccggcgat tgcagcgccg agcagatgga
      421 gctgctcgcc gatctcgccg aggcctacag caagggcgag ctccgcgtca gctatgagca
      481 gaacctcgtc ctggcccatg tccgccgcgc cgacctggcg gcgctctggc gccgcctcga
      541 cgacgccggt ctcggcaccg ccaatgcgac gctcgccagc gacatcatcg cctgccccgg
      601 cctcgattat tgcagcctcg ccaatgcccg ctcgatcccg gtcgccgagc gcctgagcgc
      661 gcatcttgcc gcgcgcgatc acgagatcgg cgagctccgc atcaagatct ccggctgcat
      721 caatgcctgc ggccatcacc atgtcggcca tatcggcatt ctcggcgtcg acaagaacgg
      781 cgaggagttc taccagatca ccatcggcgg cagcgccgac gagaatgcgg cgctgggcgc
      841 catcgtcggc cccgccgtgc ccagcgagcg cgtcaccgac gccgtcgaca cgctgatcgg
      901 cgtctatctg cgccatcgca gcgacggcga gcgcttcccc gagacctatc gccgcctcgg
      961 cgtcgcgccc ttcaaggaag cgctttatgc cgctcattaa gaacggcggc ttcgtcgcgg
     1021 atccctggcg ccacatcgag gacggcgcgc cgctgcccgc ggacgcaccg gcgaccgtgt
     1081 cctggaagcg ctggtcgagc gagcgcgaga tcctcgtcgg gcacaacggc cgcctcggcc
     1141 tccgtctccc caacgacgtg ccgccgctgt ccctggcgca agacgcgccg cgcttcgatc
     1201 tcatcgcgct cgcctttccg aaattcatcg acggccgggc cttttcgcag gcgcgcctgc
     1261 tgcgcagccg ttgcggcttc acgggcgagc tgcgcgccac cggcgatgtg ctgcgcgatc
     1321 agctgctgtt catgcggcgc tgcggcatcg acgctttcga ggtgagcgag cgcgcaatcg
     1381 ccgaggactg gcttgcggcg ctcgacgact tcgacctctt ctatcagccg gcggccgacg
     1441 gccgcccctg gatcgcacgc cagcgccttc gcgccggttg agatgggttc cctaaggacc
     1501 ccgtttgcgc caagatgacg ctagggcgcc ggcagcgacg ccgcctttga gaaaagaagg
     1561 aggacgagca tgtcactttc gatcggcgat gttgcgccgg atttcgaagc cgacacgacg
     1621 caaggccgca ttcgcttcca tgactggatc agcgacagct gggcggtgct gttctcgcat
     1681 cccaaggact tcacgccggt ctgcaccacc gagctcggct acatggcgcg gctcaagccc
     1741 gacttcgaca agcgcaacac caagatcatc ggcctcagcg tcgatgccgt gcaggaccac
     1801 gggcgctggg cgaacgacat caaggagacg cagggcttcg cgcccaacta tccgatcatc
     1861 ggcgatcccg acctcaaggt cgccaagctc tacggcatgc tgccggcgag cgcctcgggc
     1921 gacgctgcga agcgcacgcc cgccgacaat cagacggtgc gcaccgtctt cgtcatcggc
     1981 cccgacaaga agatcaagct gctgctcgtc taccccatga ccaccggccg caacttcgac
     2041 gaggtgctgc gcgtcatcga ctccttgcag ctgacggcga agcaccgcgt cgcgacgccg
     2101 gtcaactgga agcccggcga cgacgtcatc atcgccggct ccgtctccga cgacgaagcc
     2161 aagaaaatct acccccaagg ctggaaagcc ccccggccgt atatccggat cgtccctcag
     2221 ccacgctgag cgtggctgag gcgggcgagg gaatccgcac gtcggttatc aatcgataac
     2281 tttagtcaat ttcaccggcc cgctttacgc cgacttaacg ctgacggtgc taggttctcg
     2341 tcatggcgag cgacggactg agcgacaccg atcgcaagct ttctcatcta acgtctcgcg
     2401 cgcgacggcc gcggtttgga gcaggcttcc ccctcgcctc ttccggccgc ggccacgccg
     2461 tcagcccagc gcgtaatccg cctgatcctc atagattgca ccgcttttcg tcagatagct
     2521 cgcgaccaga ctgaagtgcc cgaccgtgaa aggctgggcg cggaagagcg catgctggcg
     2581 aagataggcg tggagccgct catcgctggt gccttttagg cgcgccaggg tgacgtgcgg
     2641 cgcatagcgg cgtccttcgg gcggcaggcc ctcgcgcacc atcgcgcgct cgatcttgtc
     2701 gcgcagatgg acgagcgccg gattcttctc gacgccgacc cagagcgagc gttccccgaa
     2761 ctggccgaca ccggcgagcg ccatctcgaa gcgccgcgcc ttgatctgcg ccagcgcggc
     2821 gtcgatgtcg ccggccgtgc cctcgtcgac ctcgccgatg aagcgcaagg tcacgtgatg
     2881 gttttcggga tcgacccagc gcgcgccggg gacgccggcg cagagcggcg acagccggag
     2941 cttgatctcg ggcggcagct cgatgccgac gaagaggcgc agcatggcct cacccctgcg
     3001 ctcccttgcc gccgaggagc cgctgcacat agggggcgac gcgtccgacg atgatctcga
     3061 cgccctgctt gttggggtgg atcccatcct cctggttgag gtccggcagc gtcgccacgc
     3121 cgtcgaggaa gaagggatag agcggcacgt cgtatttccg cgcgagcgcg ggatagatcg
     3181 cgtcgaattc cttctcgtag tcgtgccccc aattgggcgg cgccagcatg cccagcagca
     3241 gcaccttggc gccgctcgct ttgacgcgcg ccaggatctt gtcgagattg gcgtaggtct
     3301 gcttcggatc gatgccgcgc agcgcgtcgt tggcgccgag ctcgaccagc acgacgtccg
     3361 gatgctccgc cagcgcccag tcgagccgcg cgagcccgcc cgcgctggta tcgcccgaga
     3421 cccccgcatt ggtcatctcg accaccgtgc cgctcgcctt gagccgcgcc tgcagccgca
     3481 ccggaaacgc ctgatccggc aggagcccga accccgccgt caggctgtcc ccgaacgcca
     3541 ggacgcgcgt cccggcttca gcgaccgacg ggaacagcag tgtcgcgaac agcaagaaga
     3601 gtcccgatag cgccgggccc ctccctctcc cgcggtgcgg gagaggggcg gtcatgttga
     3661 aaacggccgg cgggtcgcca tatcggccga gggcgcgacg ctgcgcgaac aagcatctcc
     3721 gttttggaaa gaatgacatg caggcctcgc ccggttcgga cattcccatg attgagctcg
     3781 cggacgtgcg gctgacactg gcgagcgccg ccggtcccgt caacgtcctg cgcggtgtct
     3841 cgctccgcgt gg
//
LOCUS       DASYNS010000152         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4246290, whole genome shotgun sequence.
ACCESSION   DASYNS010000152 DASYNS010000000
VERSION     DASYNS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4246290"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VGP50_04800"
     CDS             complement(<1..241)
                     /locus_tag="VGP50_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8026723.1"
                     /translation="MSSVPTGETGTGQPGETQGLLAVGGHALARAFDLAHAMIREIDG
                     RIRFWSSSLERLYGWKREDAIGQISHDLLSTEFPKP"
     gene            complement(470..>1508)
                     /locus_tag="VGP50_04805"
     CDS             complement(470..>1508)
                     /locus_tag="VGP50_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV8026724.1"
                     /translation="RSVLGAAIAAALTLYAIAAKRRQRALNTVERAGNRGRGRDAEEP
                     VDMPAAGWRDVLRRLALDFGRDNLSLMAAGIAYYALVSFTPALTALVALYGLVFDPYQ
                     VAAQVASLEGMIPEEARAVIAAQLNAVVQASSSKLGIGLVVSLALALWGANSATGALM
                     SALNVVYGEHEKRSLLRYYANALALTAALVLFGIVSLVLVAIVPAVVDLLPLGDFGKT
                     LVGWSRWPLLMVLSSTGLAIIYRYAPSRAEPRWSWTSPGAAAATVLWLGGSALFSLYV
                     ETFATYNKTYGSLGAVVVLLLWLWLSALSVLIGAELNAELEHQTARDTTRRPAKPMGR
                     RGAYVADTVAP"
BASE COUNT          264 a          497 c          524 g          223 t
ORIGIN      
        1 gcggcttcgg gaattccgta ctcagcaggt catgtgatat ttgcccgatg gcgtcttcgc
       61 gcttccagcc gtaaaggcgc tcgagactgc tcgaccagaa gcgaatgcgt ccgtcgatct
      121 cgcggatcat ggcgtgggcc aagtcgaagg cacgcgcgag ggcgtggccg ccgacggcca
      181 gaagcccttg cgtctccccg ggctgcccgg tccctgtttc tccggtcgga acggagctca
      241 tcaatcaaac tcccgcgcgc acggccctct tgaaacgcga tctgcggtat ggtgaacgga
      301 gttaggcccg gctcctcccg ggccgagtga tcagcatgca tcgccgcatc actttttgat
      361 atagggcggc gccgcgtcaa tgatgttggc tagccggcac ggtgcggctt tcgcgattgc
      421 aagagcaatc gctgtcgccg ccgcggacga acgcccttcg ggcgttcgcc tagggcgcaa
      481 ccgtgtcggc gacgtaggcg ccgcggcggc ccatcggttt tgccggacga cgtgtcgtgt
      541 cgcgcgccgt ctggtgctcg agctcggcat tgagctcggc gccgatcagg acggacaatg
      601 ccgaaagcca gagccagagc agcagcacca cgaccgcgcc caacgagcca taagtcttgt
      661 tgtaggtcgc gaatgtctcg acatagagcg agaacagcgc cgatccgccg agccagagca
      721 cggtcgcggc ggcagcgccc gggctcgtcc aactccagcg cggctcggcg cggctcggcg
      781 cgtagcgata gatgatcgcg agaccggtcg atgacagcac catcagcagc ggccagcggc
      841 tccagccgac gagcgtcttg ccgaaatcgc cgagcggcag aaggtcgacg accgcgggga
      901 cgatcgcgac cagcaccagc gagacgatgc cgaacagcac gagagcggcc gtgagagcga
      961 gcgcattcgc gtaatagcgc agcaggctgc gcttctcgtg ctcgccatag acgacgttga
     1021 gcgccgacat cagcgcgccg gtcgcggagt tggcgcccca gagggccagc gccaggctga
     1081 cgacgaggcc gatgccgagt ttcgacgagc ttgcctggac gacggcgttg agctgggcgg
     1141 cgatgacggc gcgcgcttct tcggggatca tgccttcgag gctcgccacc tgcgccgcga
     1201 cctgatacgg gtcgaagacg aggccataga gcgccaccag cgccgtgagc gcgggcgtga
     1261 aggagacgag ggcgtaatac gcgatgcccg cggccatcag cgacaggttg tcccggccga
     1321 aatcgagggc gagccgccgc agcacatcgc gccagcccgc cgccggcatg tccacaggct
     1381 cttcggcgtc gcggccgcgg ccgcgattgc cggcgcgctc gacggtgttg agcgcgcgct
     1441 ggcgccgctt cgccgcaatg gcatacagcg tgagcgccgc ggcgatggcc gcgccgagca
     1501 ccgagcga
//
LOCUS       DASYNS010000153        15951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4714375, whole genome shotgun sequence.
ACCESSION   DASYNS010000153 DASYNS010000000
VERSION     DASYNS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 15951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15951
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4714375"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VGP50_04810"
     CDS             <1..695
                     /locus_tag="VGP50_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV8026725.1"
                     /translation="KQWRRVFLVVGPTEADGAYAAAVKRAITKFQAKLVDEKPWTYQP
                     GARRSDTGHYSIAAEVARFTQGINYDVLVVADEDDEFGDYLSYATTDPRPVAGTHGLI
                     AGAWARPHQEWGATQLQDRFLHRAKRWMTDRDYAAWLAVRAAGEGATRAQSIKPAEVT
                     TYMRGDKFELAGYKGLPLSFRPWDGQMRQAILLYDSRALVSVSPQPGFLHQFSTLDTL
                     GLDRPESKCRLP"
     gene            680..1675
                     /locus_tag="VGP50_04815"
     CDS             680..1675
                     /locus_tag="VGP50_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HEV8026726.1"
                     /translation="MPSALSNHHRSPAIAALALASALWSAGPLRAETVLVSNEKGNSI
                     TVLDAETLTVQKTIPVGQRPRGIVLSKDNRTLYICASDDDEIEVLDLDDFTLKPPLPS
                     GADPETFALHPDGHHLYVSNENNSLVSIIDIAQRAVVGEIPVGVEPEGIGISPDGKWV
                     VNTSETTNMAHFIDNETHKVVANVLVDPRPRRAVWTADGKQVWVSSEIGGTVSVIDAT
                     SHAIAHKIAFAVPGVPSEAIQAVGIALTKDGKLGFVALGPANRVAVVDTQSFEVKKYL
                     LVGQRVWNLAFNHDETRLFTTNGISNDISEIDVPGLRVLKSVPVGNQPWGVVVRP"
     gene            1698..2126
                     /locus_tag="VGP50_04820"
     CDS             1698..2126
                     /locus_tag="VGP50_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026727.1"
                     /translation="MRYNRFLALAPILIGLGNAPALAGSLTSQVNRLEPLILGNEQSE
                     FYMSQKEYHLTAGKYYRWKITSSGRREYNIIAPELWRNAWIYQVKVGDKEIKVPALDE
                     LDFDSAGDEEVYFVPINAGTYEFRSRGLEQRGMVGKITVE"
     gene            2131..2922
                     /locus_tag="VGP50_04825"
     CDS             2131..2922
                     /locus_tag="VGP50_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8026728.1"
                     /translation="MQATERRTETEDTASAPRDDALALEVASLSHAFGDRAALSDVSF
                     SLRRGERMVLLGLNGAGKTTLFSLITRLYDHRGGAIRIFGREIKEQPSEALARIGVVF
                     QQSTLDLDLTVAQNLFYHASLHGIARSVAAERMKHEIERVGLSDRLRDKVRELSGGQR
                     RRVEIARALLHRPQLLLLDEATVGLDIASRQFLIDHVRRLCAEENLAVLWATHLIDEV
                     ETTGKVIVLHRGKVLAQGAVPDVIRTAGAPSMRAAFDALTREPKG"
     gene            2919..3725
                     /locus_tag="VGP50_04830"
     CDS             2919..3725
                     /locus_tag="VGP50_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8026729.1"
                     /translation="MKAIHYYRCLAGIVRREGLRFLHQRGRFISALVRPLVWLLIFAA
                     GFRFVLGVSIIPPYETYVPYEVYIAPGLIGMILLFQGMQSSLSMVYDREMGSMRTLMV
                     SPLPRWFLLVAKLLAGVVVSVLQAYVFLAIAYFWEIEPPPLGYLTVLPALILAGMMLG
                     ALGLLLSSAIRQLENFAGVMNFVIFPMFFASSALYPLWHVQEASLPLYYVCEANPFTH
                     AVELIRFALYEQFDLQSALVVLGWLVVFMGLAIYGYDPARGLIARRAAAE"
     gene            3722..4339
                     /locus_tag="VGP50_04835"
     CDS             3722..4339
                     /locus_tag="VGP50_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026730.1"
                     /translation="MRSVVAVVLSALLWPEASIAQPRSPDSDWPCQQTLVPTLTAPMI
                     WSGPSPEGLGDWHGEPGVAGLVGRIAPRAVATAEGESAIQQFTRDLEGDRKRLITLAF
                     AGLLDETNRQRSEVIQRIKALAERQRNLANLVARLTTELDQTPAPANPASPDAAPRAD
                     LAQRLTFSSRAYNELQRTMRYACDVPGQLDARLGAYARALAAGLS"
     gene            complement(4349..4867)
                     /locus_tag="VGP50_04840"
     CDS             complement(4349..4867)
                     /locus_tag="VGP50_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated CXXCW motif
                     protein"
                     /protein_id="HEV8026731.1"
                     /translation="MLAGAARTEPAAVPEPSGYRLDDYRAPVPATLAGATAVGTAEAE
                     ALWREHRAVFVDVLPAPRRPEKLPPDALWKPLPRRDIPGSQWLPEVGRGALSPALETY
                     FRENLERVTQGDKAAPLVFYCLADCWMSWNATKRARSYGYTRLYWYRDGTTGWEAEAL
                     PMEETAPAPGRP"
     gene            complement(4966..5793)
                     /locus_tag="VGP50_04845"
     CDS             complement(4966..5793)
                     /locus_tag="VGP50_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV8026732.1"
                     /translation="MSRRIVVCLCGLALLGWAGPAAPQSATGNLVARSELRVCADPSN
                     LPFSDEKGEGFENKIAALLAQDMSLPVKYTFFPQVVGFVRNTLRAGQCDLVMGTVAGD
                     DVMQTTTPYYYTSYVLVMPKDKALSFSGFDDPRLRDLHIGIIAGTPPADLLVRYGLMA
                     NARPYALMVDTRFESSTHQLMTDVAEGKVDAAMLWGPIAGYYVKRDSLPLTLAPIKSE
                     PGLPRMDYHIAMGVRANEPDWRRRINDEIRKNETKINEILRDYGVPLLDEQGAALKP"
     gene            complement(5794..6222)
                     /locus_tag="VGP50_04850"
     CDS             complement(5794..6222)
                     /locus_tag="VGP50_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV8026733.1"
                     /translation="MTVRLMMGALAGSWVLALTIATAAAPAEEAKPYTVKDGKVDQHT
                     YNGWRRYTESCLRCHGPDGAGSSYAPDLVESLKSLDEDQFKEIVVNGRKNVTTSAENV
                     MPAFGEVEDVVLYLDDMYAYLKARSDGVLGRGRPERISEQ"
     gene            complement(6342..8123)
                     /locus_tag="VGP50_04855"
     CDS             complement(6342..8123)
                     /locus_tag="VGP50_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol/ethanol family PQQ-dependent
                     dehydrogenase"
                     /protein_id="HEV8026734.1"
                     /translation="MQPRGYLLVAAALAIPVSGALANDELMKLEKDPNQWVMPTGNYA
                     NQRYSELKKINKENVKNLRPVWTFSTGVLRGHEGAPLVIGNIMYVHTPFPNYVYALDL
                     DKGGEIIWKYEPRQDPSVIPVMCCDTVNRGVAYADGKIILNQADATVVALDAKSGKEV
                     WKVKNGDAKKGETMTSAPQIIKDKVLVGISGGEFGVQGRMTAYNLKDGSQAWIAYSVG
                     PDDQILFDDKTTSMGKPVGKDSSLATWQGDQWKIGGGATWGWISYDPKLNLVYYGSGN
                     PSTWNPVQRPGDNKWSMTIFARNPDTGVAKWVYQMTPHDEWDYDGINEMILADIPVKG
                     QPTKALVHFDRNGFGYTLNRETGALLVAEKYDPKVNWAKRVDMATGRPEVVSQYSTGT
                     GGEDHNSTQICPAALGSKDQQPAAFSPETKLFYVPTNHVCMDYEPFKVSYTAGQPYVG
                     ATLSMYPPKGDENLGNFIAWDAGQGKIVWADKEPFSVWSGALATAGGIVFYGTLEGYL
                     KAVDINTGKELYRFKTPSGIIGNVMTYEHHGKQYIAVLSGVGGWAGIGMAAGLTKDTD
                     GLGAVGAYKALSSYTQLGGVLTVFGLD"
     gene            complement(8309..9112)
                     /locus_tag="VGP50_04860"
     CDS             complement(8309..9112)
                     /locus_tag="VGP50_04860"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HEV8026735.1"
                     /translation="MIRLTLKAPLPHRIAADRLIPERLAALSTREVERLPLALGNRPA
                     QVADLFRVEGTADGAVEIVGLCRRVDRIGAGMSGGTITVRGDAGAYLGLGMRGGGITV
                     EGSVDFGAATALEGGMLRISGDAGDGLAGGLPGAVRGMNGGTVLVGGSAGAAVGQRLR
                     RGLIVIGGAAGACCGSEMIAGTIVIGGTLGADAGIAMRRGSIVALGGVDRIAPTFVDC
                     GVHDLVWLRLLRRHIELLGEARLAERIQSLRRFAGDAAVVGRGELLLPR"
     gene            complement(9109..10005)
                     /gene="fhcD"
                     /locus_tag="VGP50_04865"
     CDS             complement(9109..10005)
                     /gene="fhcD"
                     /locus_tag="VGP50_04865"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HEV8026736.1"
                     /translation="MKLEGIAIDDTFAEAFGMRGTRLVITADSLPWAEVAGRSMTGFA
                     TSVIGCGLEAGIERALVPEETPDGRPGIAVLLFAVSRDALAKALRDRVGQCVLTCAGT
                     ACFGGLMSADQVPLGRTLRYFGDGHQTAKRIGERRYWRIPVMDGEFLCEDQVGIQKAV
                     GGGNFLILGRTRPGVLAAAERAIAAMNRVPEAIAPFPGGIVRSGSKVGSRYKFLPAST
                     NDAFSPTLRGVVASELGPEENAVLEIVIDGLSDKAVAAAMQAGIRAACAGGAADGILR
                     ITAGNYGGKLGAYKFALRELLA"
     gene            complement(10002..11666)
                     /locus_tag="VGP50_04870"
     CDS             complement(10002..11666)
                     /locus_tag="VGP50_04870"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HEV8026737.1"
                     /translation="MITVLAQGRVFDPASGVKGETRDLFIEDGRIIAPPQVRRADRRY
                     DLGGRIVMAGAIDIHSHVAGGKVNLARLLMSEDHRAVVAERDALRRSGSGVATPSTFL
                     TAYRYATMGYTAAFEPAMVPANARSTHLEMVDLPLVDKGAYVMLGNDELLLRLLAEGA
                     EQERINDYVAWMMRATASLGIKTVNPGGISAFKFNGRGLDIDQPGPQHGVAPRRIIAA
                     LARAAYELGVPHPLHTHCNNLGIPGNIETTLATIAAAEGFPLHLTHVQFHSYGTEGDR
                     HFSSAAARLAEAVNRNPNITLDVGQIMFGQTVTASGDTMAQYRNRVFASPKKWLSMDI
                     ECDGGCGVVPFRYRDKNFVNALQWAIGLELFLLIDDPWRIFLTTDHPNGAPFTAYPTL
                     IRLLMDRSYRDDCLKAIHPAAQQATALASIAREYTLEEIAVMTRAAPARILGLADRGK
                     LAPGAAADITVYTEDADRERMFGQPHLLFKDGVEVMREGALVAVPKGVTHVVRPDFDA
                     AIEKRIARFFDDHMSVGFAHFPLSPTELADAGIDLVAHPCRPRGRA"
     gene            complement(11663..12895)
                     /locus_tag="VGP50_04875"
     CDS             complement(11663..12895)
                     /locus_tag="VGP50_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase"
                     /protein_id="HEV8026738.1"
                     /translation="MSAVPVRVACPFCGLVCDDLIVDGERVDGRGCAKAAANFSRRVT
                     TSEHRIAGRAASLDDAVAAAAALLRGAGQPLVTGLGADLSGMRALLALAERLGAVVDR
                     WRSAPQFANFAVLQRAGMLAATFAEIANRTDLALIVGSDPARSYPRFFERLLRNATPL
                     YRKAAPSVVYLGPEVAAPTDPAVGERIAVEGDRLPDALRALAAAIEGRRVAADPDVKL
                     AALVALARRLESASYGTIVWDAAELPALAREDIASLILRVLRTLTHKTRCVGLPLGGN
                     DNAQGVAQAMLWQTGWPGRLSFATGTPIHDPWLYDAERLLREGEVDVLLWVQAFGVEL
                     PPATRVPTIALLSADVAPPAPAAVEIRIGVPGLDHRGWVMRSDTVVALPLAETRSAPL
                     PSVATVATAIHRHVEAQP"
     gene            complement(12892..13299)
                     /locus_tag="VGP50_04880"
     CDS             complement(12892..13299)
                     /locus_tag="VGP50_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEV8026739.1"
                     /translation="MAERPRERTYSEDEIKARLAKELPHWYYEDGWIRRKYRTHGWKG
                     TLMVINAVGHLAEAAWHHPDITASYAWVEVRLMNHAAKGVSDKDFELARKIEDVVHWQ
                     PASAGGALEGTPQDDQRFAYIKYDKPGDSARGG"
     gene            13436..14419
                     /locus_tag="VGP50_04885"
     CDS             13436..14419
                     /locus_tag="VGP50_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase family protein"
                     /protein_id="HEV8026740.1"
                     /translation="MGGENAAVEVFAPARLHLGFLDLEGALGRRYGSLGLAIDRFGTR
                     LTLTRAKRASAEGPAADRARSYLERAAASLGVPPAARVAIEEASPEHAGFGSGTQLGL
                     AVAAALAHLHGLTVATPALAAAIGRGARSGIGIGAFDLGGFIVDGGLKSGAEYPPVTA
                     HLAFPAAWRILLILDHGRSGLHGDAERAAFSALPPFTEALAGRLCRLVLMSLLPGLAE
                     EDFTAVSHTLGEIQSRLGDYFSPAQNGRFSSPAVADALTWLGAQGITGTGQSSWGPTG
                     FALFESAAEATRMAERLSEEPGSRALELQVVAGRNAGAEIAARRAVKEARR"
     gene            14416..15306
                     /locus_tag="VGP50_04890"
     CDS             14416..15306
                     /locus_tag="VGP50_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent
                     methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HEV8026741.1"
                     /translation="MTTPRILHFLTPLANVSPFDVNMAVDAGFVVASYTNISLKEVTA
                     LTQDAMFSRSPADAAKTCLFIGGRDALQALDMLAAAKKAMFPPFEISLFADPSGAFTT
                     AAAMVACVERQLKAQAATLAGAEVAVFGAKGVVGGIVGVIAAEAGAHVVLVAHDKSGV
                     VEAKAAEFERRFGRSFAVADGSSEAGRRGVLKNAEVVLACGRAGLRILSRAEMSAAQR
                     LKVAADINAVAPPGIEGLDVKAKGTKLPGTPGMGIGALAIGNVKFQLQHALLKRLDEA
                     QKAQCFDFRDAYRVALELVH"
     gene            15296..>15951
                     /locus_tag="VGP50_04895"
     CDS             15296..>15951
                     /locus_tag="VGP50_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026742.1"
                     /translation="MSTEAQPALAVALSARSLAAAARRAGACVIALDLFADEDTAAAA
                     RACRKLAPLTLPQGERGGLFAFDREALLEAVEEHRGKASGLVYGAGFEHEPALLGMLE
                     SRIAILGNRAEVIGAVKDPCGFAALLARLGVPHPETRLEAPAEGNWLTKRIGGSGGGH
                     ITRASAGAIGAGFYVQRQVAGRAVSALFVGDGRRARVLGHSEQWTSPTAEMPFRYGGC
                     V"
BASE COUNT         2640 a         5341 c         5287 g         2683 t
ORIGIN      
        1 caaagcagtg gcggcgcgtg ttcctggtcg tcggcccgac cgaggcggac ggcgcctatg
       61 ccgcagcggt caaacgcgcg atcacgaagt tccaggcgaa gctggtcgac gagaagcctt
      121 ggacctatca gcccggcgcg cggcgctccg atacgggcca ttactcgatt gccgccgagg
      181 tcgcgcgctt cacgcaaggc atcaactatg acgtcctcgt cgtcgccgac gaggacgacg
      241 aattcggcga ttatctgagc tatgccacta cggacccgcg gccggtcgcc ggaacccacg
      301 gcctcatcgc cggcgcctgg gcgcggccgc atcaggaatg gggcgcgacg cagctgcagg
      361 accgattcct ccaccgcgcg aagcgctgga tgacggatcg cgattatgcc gcctggctgg
      421 cggtgcgcgc ggcgggagag ggagcgacgc gcgcgcagtc gatcaagccg gcggaggtca
      481 cgacgtatat gcgcggcgac aagttcgagc tcgcgggata caaggggctg ccgctcagct
      541 tccgcccctg ggacgggcag atgcggcagg cgatcctgct ctatgattcg cgcgcgctcg
      601 tctccgtgtc gccgcagccg ggcttcctgc accagttctc gacgctcgac acgctgggcc
      661 tcgaccgccc cgaatcgaaa tgccgtctgc cctgagcaac caccatcgat ctccggcgat
      721 cgctgcgctc gcactggcgt cggcgctgtg gtcggcgggt ccgctccgcg cagagacggt
      781 gctcgtctcg aacgagaaag gcaattccat caccgtgctc gatgccgaga cgctgacggt
      841 gcagaagacc atcccggtcg gccagcggcc gcgtggcatc gtcctctcca aggacaatcg
      901 cacgctctat atttgcgcca gcgacgacga cgagatcgag gtgctcgacc tcgacgactt
      961 cacgctgaag ccgccgctgc cgagcggcgc cgatcccgag acctttgcgc tccatccgga
     1021 cgggcaccat ctctatgtct cgaacgagaa caacagcctc gtctcgatca tcgacatcgc
     1081 gcagcgtgcg gtcgtcggcg agattccggt gggagtcgaa cccgaaggca tcggcatcag
     1141 cccggacggg aaatgggtgg tgaacacctc cgagaccacc aacatggcgc atttcatcga
     1201 caacgaaacg cataaggtcg tcgccaatgt tctcgttgac ccgcggccgc gccgcgcggt
     1261 ctggaccgcg gacggcaagc aggtctgggt ctcttccgag atcggcggca ccgtgagcgt
     1321 catcgatgcg acgtcgcatg cgatcgcgca caagattgct ttcgccgtgc ccggcgtccc
     1381 gagcgaagcg atccaggccg tcggcatcgc gctcaccaag gacgggaagc tcggcttcgt
     1441 cgccctcggt ccggccaacc gcgtcgccgt cgtggacact cagagcttcg aggtgaagaa
     1501 gtatctgctg gtggggcagc gcgtgtggaa tctcgccttc aaccacgacg aaacgcggct
     1561 cttcaccacg aacggcatca gcaacgacat ctccgagatc gacgtgccgg gcttgcgcgt
     1621 cctgaaatcg gtgccagtcg gcaatcagcc ctggggcgtg gtcgtgcgtc cctgagcgaa
     1681 agccagagga gcggaggatg cgatacaaca ggttcttggc gctcgcaccg atcctgatcg
     1741 ggcttggcaa cgcgcccgcg ctcgccggca gcctcaccag ccaggtgaac cggctcgagc
     1801 cgctgatcct cggcaacgag cagagcgaat tctatatgtc acagaaggag tatcatctga
     1861 cggccgggaa atattatcgc tggaagatca cctcgagcgg ccggcgcgaa tacaacatca
     1921 ttgcgccgga actctggcgc aatgcctgga tctaccaggt gaaggtcggc gacaaggaga
     1981 tcaaggtgcc ggcgctcgac gagctcgact tcgacagcgc cggcgacgag gaagtgtatt
     2041 tcgtgccgat caatgccggc acctatgaat tccgctcgcg cggcctcgag cagcgcggca
     2101 tggtcggcaa gatcaccgtg gagtgacggg gtgcaggcga ccgagcgccg gacggagacg
     2161 gaagacaccg cgagcgcgcc gcgcgacgac gcgctcgccc tcgaggtcgc cagcctctct
     2221 catgccttcg gcgaccgcgc ggcgctgagc gacgtctcct tcagtctccg tcgcggcgag
     2281 cgcatggttc tgctcgggct caatggcgcc ggtaagacca cgctcttctc gctcatcacg
     2341 cggctctacg atcaccgcgg cggcgcgatc cgcatcttcg gccgcgagat caaagagcag
     2401 ccgtcggaag cccttgcccg catcggcgtc gtgttccagc agtcgacgct cgatctcgac
     2461 ctgaccgtcg cgcagaacct cttttaccat gcatcgctcc acggcatcgc gcgcagcgtc
     2521 gcggccgagc ggatgaagca cgagatcgag cgcgtcggct tgagcgatcg cctgcgcgac
     2581 aaggttcgcg agctgagcgg cggccagcgg cgccgcgtcg agatcgcgcg ggcgctgctc
     2641 catcgcccgc agcttctgct gctggacgag gcgaccgtcg ggctcgacat cgccagccgg
     2701 caattcctca tcgatcatgt gcgccggctc tgcgcggagg agaatcttgc ggtgctctgg
     2761 gcaacccatc tcatcgacga agtcgagacg acgggcaagg tgatcgtgct ccatcgcggc
     2821 aaagtcctgg cgcaaggcgc cgttcccgac gtgatccgca ccgccggcgc tccctctatg
     2881 cgtgccgcgt tcgacgccct gacgcgggag ccgaagggat gaaagccatc cattattatc
     2941 gctgtctcgc cggcatcgtg cggcgcgagg gcttgcgctt cctgcatcaa cgcggccgct
     3001 tcatctcggc gctggtgcgg ccgctggtct ggctgctgat cttcgccgcc ggattccgtt
     3061 tcgtgctcgg cgtctcgatc atcccgcctt acgagaccta tgtgccctac gaggtctata
     3121 tcgcgcccgg cctcatcggc atgatcctgc tgttccaggg catgcagagc tcgctctcga
     3181 tggtctatga ccgcgagatg ggcagcatgc gcacgctcat ggtgagcccg ctgccgcgct
     3241 ggttcctgct ggtggcgaag ctgctggccg gcgtcgtcgt ctcggtgctc caggcctatg
     3301 tctttctcgc catcgcctat ttctgggaga tcgagccgcc gccgctggga tatctgacgg
     3361 tgctgccggc gctcattctc gccggcatga tgctgggcgc gctcgggctg ctgctgtcgt
     3421 cggcgatccg ccaactcgag aacttcgcgg gcgtgatgaa cttcgtgatc ttcccgatgt
     3481 tcttcgcgtc gtccgcgctt tatcctctct ggcacgtcca ggaggcgagc ttgccgctct
     3541 actatgtctg cgaagcaaac cccttcaccc acgccgtcga gctcatccgc ttcgcgcttt
     3601 acgagcaatt cgacctgcag tcggcgctcg tcgtgctggg ctggctcgtc gtattcatgg
     3661 gtctggcgat ctatggctat gatcccgcgc gcggcctgat cgcgcgccgc gcggcggcag
     3721 aatgagaagc gtcgtcgccg tcgtgctctc ggcgttgctc tggccggagg cgagcatcgc
     3781 gcaaccgcgc agccccgact cggactggcc gtgccaacag acactcgtgc cgacgctgac
     3841 ggcgcccatg atctggagcg gcccctcgcc cgaaggcctc ggcgactggc atggcgagcc
     3901 cggcgtggcc gggctggtcg ggcgcatcgc gccgcgcgcc gtggcgacgg cggagggcga
     3961 aagcgccatc cagcaattca cgcgcgacct cgagggcgac cgcaagcggc ttatcacctt
     4021 ggccttcgcc ggcctcctcg acgagaccaa ccgccagcgc agcgaagtga tccagcgcat
     4081 taaggcgctc gccgagcgcc agcgcaacct cgccaacctc gtcgcgcggc tcacgacgga
     4141 gctcgatcag acaccggcgc cggccaaccc ggcgagcccc gatgcagcac cgcgcgccga
     4201 cctcgcccag cgcctgacct tcagctcccg cgcctacaac gagctgcagc gcacgatgcg
     4261 ctacgcctgc gacgtgccgg gccagctcga cgcgcggctc ggcgcttatg cgcgcgcgct
     4321 cgcggcggga ttgtcgtagc gccgtggctt agggacggcc gggcgccggc gccgtttctt
     4381 ccatcggcaa cgcttccgcc tcccaccccg tggtgccgtc gcgataccag tagagccgcg
     4441 tatagccata agagcgtgcg cgcttggtcg cgttccagct catccagcaa tcggcgagac
     4501 aataaaagac gagaggcgcc gctttgtcgc cctgagtgac gcgctccagg ttctcgcgga
     4561 aataagtttc gagcgccgga ctgagcgcgc cccggccgac ttccggcagc cactggcttc
     4621 cgggaatgtc gcggcgcggc agcggcttcc acagcgcgtc cggcggcagc ttctcggggc
     4681 ggcgcggcgc cggcagcaca tcgacgaaga cggcgcggtg ctcgcgccac agcgcctcgg
     4741 cttccgccgt gccgaccgcg gttgcgccgg cgagtgtcgc cggcacgggc gcgcgataat
     4801 cgtcgaggcg gtagccggag ggctcgggca cggcggcggg ctcggtgcgc gcggcgccgg
     4861 cgagcacgca cgataacgtc gacgccagcg cgatgctcag aaacagagcg agtccccctc
     4921 tccccttgcg ggagagggag gggaggtggg cccccctcaa ccgggtcaag gcttgagagc
     4981 ggcgccttgc tcgtcgagga gcggcacgcc gtagtcgcgc aggatctcgt tgatcttcgt
     5041 ctcgttcttg cggatctcgt cgttgatgcg gcggcgccag tcgggctcgt tggcgcgcac
     5101 gcccatggcg atgtggtagt ccatgcgcgg caagcccggc tcgctcttga tgggcgcgag
     5161 cgtcaggggc aggctgtcgc gcttgacgta atagcccgcg atcgggcccc agagcatggc
     5221 ggcatcgacc ttgccttcgg cgacgtcggt catgagctgg tgcgtcgacg attcgaaccg
     5281 cgtatcgacc atgagcgcat aaggccgggc atttgccatg aggccgtagc gcaccaagag
     5341 atcggccggc ggcgtgccgg cgatgatgcc gatgtgcaaa tccctgagcc tcgggtcgtc
     5401 gaagccggaa aaggacagcg ccttgtcctt cggcataacc aggacgtagc tcgtgtagta
     5461 atagggcgtc gtcgtttgca tcacgtcgtc gccggcgacc gtgcccatga cgagatcgca
     5521 ttgcccggcg cgcagcgtgt tgcgcacgaa gccgacgact tgcggaaaga aggtgtactt
     5581 caccggcagc gacatgtcct gcgccaggag cgccgcgatc ttgttctcga agccctcgcc
     5641 cttttcgtcg gagaaaggca ggttgctggg gtcggcgcag acgcgcaatt cgctgcgcgc
     5701 gaccagattg ccggtcgccg actgaggagc ggcgggacct gcccagccga gcaaggcgag
     5761 cccgcaaaga cacacgacga tgcgccggga catctattgc tccgagatgc gctccgggcg
     5821 gccgcgcccg agcacaccgt ccgagcgcgc tttgagataa gcgtacatgt catcgagata
     5881 gagcacgaca tcctcgacct cgccgaaggc cggcatgaca ttctcggccg aggtcgtcac
     5941 gttcttccgc ccgttgacga cgatctcttt gaactggtcc tcgtccagcg acttcagcga
     6001 ctcgacgaga tccggcgcgt aggagctgcc ggcgccatcg ggtccatggc agcgcaggca
     6061 ggattcggtg tagcgccgcc agccgttata ggtgtgctgg tcgaccttgc cgtccttgac
     6121 cgtataaggc ttcgcctctt ccgccggagc ggcggcggta gcgatcgtga gagcgagcac
     6181 ccacgagccc gcgagtgctc ccatcatcag gcgcaccgtc atctcctccc ttgaagctgg
     6241 ccttggttcg gacgcgccgt ccaaggcaca gagaaaaagg ccggcacccc ggtttcccga
     6301 agatgccggc cgcaaccaag aggtttcgcg ttaccgagtg ctcaatcgag gccgaacacc
     6361 gtcagaacgc cgccgagctg cgtgtagctg gagagcgcct tgtacgcgcc caccgcgccc
     6421 aggccgtcgg tgtccttggt gagacccgcg gccatgccga tgccggccca gccgccgacg
     6481 ccggacaaca cggcgatgta ctgcttgccg tgatgctcat aggtcatcac gttgccgatg
     6541 atgccggacg gcgtcttgaa gcgataaagc tccttgccgg tgttgatgtc gacggccttc
     6601 agatagccct cgagcgtgcc gtagaagacg atgccgccgg ccgtggcgag cgcgcccgac
     6661 cagaccgaga agggctcctt gtccgcccag acgatcttgc cctgtcccgc atcccaggcg
     6721 atgaagttac cgagattctc gtcgcccttc ggcggataca tcgacagcgt cgcgccgaca
     6781 tagggctgac ccgcggtgta gctcaccttg aagggctcgt agtccatgca aacgtggttg
     6841 gtcggcacgt agaacagctt ggtctccggc gagaaggctg ccggctgctg atccttcgaa
     6901 cccaacgccg ccgggcagat ctgcgtcgag ttgtggtcct cgccgcccgt ccccgtgctg
     6961 tactggctta ccacttccgg acggccggtg gccatgtcga cacgcttcgc ccagttcacc
     7021 ttgggatcgt atttctcggc gacgaggagc gcgcccgtct cgcggttcag ggtgtagccg
     7081 aagccgttgc ggtcgaaatg gaccaatgcc ttcgtcggct gacccttcac cgggatgtcg
     7141 gcgaggatca tctcgttgat gccgtcatag tcccattcgt catggggcgt catctgatag
     7201 acccacttcg cgacgccggt atcgggattg cgcgcgaaga tggtcatcga ccatttgttg
     7261 tcgccgggcc gttgcaccgg gttccaggtg ctgggattgc ccgatccgta atagaccaga
     7321 ttgagcttcg gatcgtagga gatccagccc caggtcgcgc cgccgccgat cttccactgg
     7381 tcgccctgcc aggtggcgag cgaggaatcc ttgccgaccg gcttgcccat cgacgtggtc
     7441 ttgtcgtcga acaggatctg atcgtcgggc ccgaccgaat aagcgatcca ggcttgcgat
     7501 ccgtccttga ggttataagc ggtcatgcgg ccttgcacgc cgaactcgcc gccgctgatg
     7561 ccgacgagca ccttgtcctt gatgatctgc ggggcgctcg tcatggtctc gcccttcttc
     7621 gcatcaccgt tcttgacctt ccatacctct ttgccgctct tggcatcgag ggcgacgacg
     7681 gtcgcgtcgg cctgattgag gatgatcttg ccgtcggcat aggcgacgcc gcgattgacg
     7741 gtatcgcagc acatcaccgg aatcaccgag gggtcctgcc tcggctcgta tttccagatg
     7801 atctcgccgc ccttgtcgag gtcgagggcg tagacataat tggggaacgg cgtgtgcaca
     7861 tacatgatgt tgccgatcac cagcggcgca ccctcatggc cgcgcagaac gccggtcgag
     7921 aatgtccaga ccggacgaag atttttgacg ttctccttgt tgatcttctt gagctcgcta
     7981 tagcgctggt tcgcgtagtt gccggtcggc atcacccatt gattgggatc tttctcgagc
     8041 ttcatcagct cgtcattcgc caacgccccg ctcacaggaa tcgccagggc agcggctacc
     8101 aacaggtacc ctctgggttg catcgaatcc tcccaccatt ggactgtttc gagctgctct
     8161 accgcgtagc tgcaagacgg cagcggccca gccgaggcgg tcgcggagat cgcgtcctct
     8221 tagacgctct ctatagcggc tgacgagatg ataagcccat tgcaatgctt cgcacaagcg
     8281 ccttgaaatc ttgagcgtcc ccgtcgattc atcgcggcaa caacaattca ccgcgcccca
     8341 cgaccgccgc atcgccggcg aagcgccgca gcgattggat gcgctccgcc agtcgcgcct
     8401 cgcccagcag ctcgatatgg cggcgcaaca agcgcagcca gaccaggtcg tgcacgccgc
     8461 aatcgacgaa agtcggcgcg attctgtcga ctccgccgag cgcaacaata ctgccgcgcc
     8521 gcatcgcgat cccggcatcg gcgcccagcg tgccgccgat gacgatggtc ccggcgatca
     8581 tctcgctacc gcaacatgca ccggccgcgc cgccgatcac gatgagaccg cgtcgcagcc
     8641 tttgtccgac agcagccccg gcgctgcccc cgacgagaac tgtgcctccg ttcattccgc
     8701 ggacggctcc cggcaagcca ccggcgaggc cgtcgccagc gtcgccggat atccggagca
     8761 tcccgccttc gagcgctgtc gcagcgccga aatcgaccga gccctcgacg gtgatgccgc
     8821 cgccgcgcat gccgaggccg agataggcgc cggcatcgcc cctgacggtg atcgtgccgc
     8881 cgctcatgcc ggcgccgatc cggtcgacgc ggcggcatag accgacgatc tcgacggcgc
     8941 cgtcggcggt gccttcgacg cggaacaagt cggcgacctg cgccggccga ttgcccaaag
     9001 cgagcggcag cctttcgacc tcgcgcgtcg agagcgcggc cagccgctcc gggatcagcc
     9061 ggtcggcagc gatccggtgc ggcagcggcg ccttgagcgt gagccggatc acgcgaggag
     9121 ctcgcgcagc gcgaacttgt aggcgccgag cttgccgccg tagttgccgg cggtgatgcg
     9181 aagaatgccg tccgccgcgc cgccggcgca ggcggcgcga atgcccgcct gcatcgctgc
     9241 ggcgacggcc ttgtcgctca agccgtcgat gacgatctcg agcacggcat tctcctcggg
     9301 gccgagctcg ctcgcgacga cgccgcgcaa ggtcggagag aaggcgtcat tggtcgaggc
     9361 cggcaggaat ttgtagcgcg agccgacttt ggaccccgag cgcacgatgc cgcccggaaa
     9421 cggcgcgatc gcttcgggga cgcggttcat ggcggcgatg gcgcgctcgg ccgcggcgag
     9481 aacgcccggg cgcgtgcggc cgagaatcag gaaattgccg ccgccgaccg ccttctggat
     9541 gccgacctgg tcttcgcaca gaaactcgcc atccatcacc gggatgcgcc agtaacgacg
     9601 ctcgccaata cgctttgccg tctgatggcc gtcgccgaaa taacgcaagg tgcgcccgag
     9661 cggcacctga tccgcgctca tgagcccgcc gaaacacgcg gtcccggcac aagtgaggac
     9721 gcattggccg acgcggtcgc gcaacgcctt ggcgagcgca tcgcgcgata cggcgaaaag
     9781 cagcacggcg atgccgggcc ggccgtccgg cgtctcctcc ggcacgagcg cgcgctcgat
     9841 accggcctcg aggccgcagc cgatcaccga ggtggcaaag ccggtcatgc tccgtccggc
     9901 gacctcggcc catggcaggc tgtcggcggt gatgacgagg cgcgtgccgc gcatgccgaa
     9961 ggcctcggcg aaagtgtcgt cgatggcgat accctcgagc ttcacgcgcg ccccctggga
    10021 cggcagggat gggcgacgag gtcgattccg gcatccgcga gctcggtggg cgacaagggg
    10081 aaatgagcga agccgacgct catgtgatcg tcgaagaagc gggcaatccg cttttcgatg
    10141 gcggcgtcga aatccggccg gacgacatgc gtgacgccct tcggcacggc gaccagcgcc
    10201 ccctcgcgca tcacttcgac cccatccttg aacaggagat ggggctggcc gaacatgcgc
    10261 tcgcgatccg cgtcctcggt atagacggtg atgtcggcag cggcgcccgg cgcgagcttc
    10321 ccgcgatcgg cgaggccgag aatgcgcgcc ggcgctgcgc gcgtcatgac agcgatctcc
    10381 tcgagcgtat attcgcgcgc gatcgaagcc agcgcggtcg cctgttgcgc ggcgggatgg
    10441 attgccttca agcagtcgtc gcggtaactg cgatccatca ggaggcggat gagcgtcgga
    10501 taggcggtaa aaggcgcacc attcggatgg tcggtggtga ggaagatgcg ccagggatcg
    10561 tcgatcagca ggaacagctc gagcccgatc gcccattgca gcgcattgac gaagttcttg
    10621 tcgcggtagc gaaaggggac gacgccgcag ccgccgtcgc attcgatgtc catcgacagc
    10681 catttcttgg gcgaggcgaa tacgcggttg cggtattgcg ccatggtgtc gcccgaggcg
    10741 gtgaccgtct ggccgaacat gatctggccg acatcgagcg taatattcgg gttgcgattg
    10801 accgcctcgg cgagccgggc cgccgccgaa gagaaatggc ggtcgccctc cgtgccatag
    10861 ctgtggaact gcacatgggt gaggtgcagc gggaagccct cggccgcggc aatggtggcg
    10921 agcgtcgtct cgatgttccc gggaatgccg agattgttgc aatgggtgtg gagcggatgc
    10981 gggacgccga gctcataggc ggcccgcgcc agagcggcga tgatgcggcg cggcgcaaca
    11041 ccgtgctgcg gtcccggctg gtcgatgtcg agaccgcgcc cattgaactt gaaggcgctg
    11101 atgccgccgg gatttaccgt cttgatgccg agcgacgcgg tcgcccgcat catccaggcg
    11161 acatagtcgt tgatgcgctc ctgctcggcg ccctcggcca ggagccgcag cagcagctcg
    11221 tcattgccga gcatcacata ggcgcctttg tcgacgagcg gcaggtcgac catctcgaga
    11281 tgcgtcgagc gtgcgttggc cggcaccatc gccggctcga aggcggcggt gtagcccatc
    11341 gtcgcgtagc gataggcggt caggaaggtc gagggcgtgg caacaccgct gcccgagcgc
    11401 cggagcgcgt cacgctcggc gaccacggcg cgatggtcct cgctcatcag caaccgggca
    11461 agattgacct tgccgccggc gacatggctg tggatgtcga tggctcccgc catgacgatg
    11521 cgaccgccga gatcgtagcg acgatcggcc cgccggacct ggggcggcgc gatgatgcgt
    11581 ccgtcctcga tgaagagatc acgcgtctcg cccttgacgc cgctcgcggg gtcgaagaca
    11641 cgaccttgcg cgagcacggt gatcatggtt gcgcctcgac gtggcggtga atggcggtag
    11701 cgaccgtggc gacactcggc agcggcgccg agcgcgtctc ggcgagcggc agagcgacca
    11761 cggtatcgct gcgcatcacc cagccgcgat ggtcgaggcc gggtacgccg atgcggatct
    11821 cgaccgcggc gggagcgggt ggcgcgacat cggcggacag gagggcgatg gtcggcacgc
    11881 gcgtcgccgg cggcagctca accccgaagg cctgcaccca cagcaggaca tcgacctcgc
    11941 cttcgcgcag cagacgctcc gcgtcataga gccatggatc atggatcggc gtgcccgtag
    12001 cgaaagagag ccggccgggc cagcccgtct gccacagcat ggcctgggcg acgccttggg
    12061 cattgtcgtt gccgccgagc ggcaacccga cgcaccgggt cttgtgggtc agcgtccgca
    12121 acacccggag gatgaggctg gcgatgtcct cgcgggccaa tgcgggcagc tcggcggcat
    12181 cccagacgat ggtgccgtag ctcgcagact cgaggcgccg agccaacgcc acgagcgcgg
    12241 ccagcttcac atcgggatca gcggcgacgc gccgcccctc gatcgcggcg gcgagggcgc
    12301 gaagcgcatc cggcagccga tcgccctcga ccgcgatgcg ctcgccgacc gcgggatcag
    12361 tcggcgcagc aacctccggc cctagataga cgaccgaggg tgccgccttg cgataaagcg
    12421 gagtggcgtt gcgcagcagc cgctcgaaga accgcggata gctccgcgcg ggatcgctgc
    12481 cgacgatgag cgccagatcg gtacggttgg cgatctcggc gaatgtcgcc gccagcatgc
    12541 ccgcgcgctg cagcacggcg aaattcgcga attgcggcgc cgatcgccag cgatcgacga
    12601 cggcgccgag gcgctcggca agcgccagca gcgcacgcat gcccgagagg tcggcaccca
    12661 gtccggtaac cagcggctgg cccgcgccgc gcagcagagc cgcagccgcc gcgacggcgt
    12721 cgtcgagcga cgccgcccgc ccggcgatgc gatgctcgct cgtcgtgacg cgccgcgaaa
    12781 aattcgccgc ggccttggca cagccgcgcc cgtcgacgcg ctcaccgtcg acgatcaggt
    12841 cgtcacaaac aagtccgcag aagggacaag cgacgcgcac cggcacggcg ctcacccccc
    12901 gcgcgcgcta tctcccggct tgtcgtattt gatgtaggcg aagcgctgat cgtcttgcgg
    12961 cgttccctcg agcgcgccgc cggcgctggc gggctgccaa tggacgacgt cctcgatctt
    13021 gcgtgcgagc tcgaaatcct tgtcgctcac gcctttcgcg gcatggttca tcaaccgcac
    13081 ttcgacccag gcgtaagaag ccgtgatgtc cggatggtgc cacgccgctt cggccaggtg
    13141 accgacggcg ttgatgacca tcagcgtgcc cttccaccca tgtgtgcggt atttgcgccg
    13201 gatccaacca tcctcgtagt accaatgcgg cagttccttc gcgagccgcg ccttgatctc
    13261 atcctcgctg taggtgcgct cacgcgggcg ttcggccatg tcgacctccc cattttgcgc
    13321 tgcaccgcgc cgatccaaca tcgcgccgag ccgctttgtc tcttctggct tttagtctat
    13381 cgtaaagcgg atgcttcgca atagcttcgc gacgacaaca gcggagaaga cgaagatggg
    13441 aggagaaaac gccgccgtcg aggtttttgc gccggcgcgt cttcatctgg gatttctcga
    13501 cctcgaaggc gcgctcggac gacgctatgg gagtctcgga ctcgcgatcg accgcttcgg
    13561 cacgcggctg acgctgacgc gcgcgaagcg cgcatcggcc gagggccctg ccgccgatcg
    13621 cgcgcgctcc tatctcgaac gcgccgccgc gtcgctcggc gtgccaccgg ccgcacgcgt
    13681 tgcgatcgag gaggcaagcc ccgagcacgc cggcttcggc tccggcacgc agcttggcct
    13741 tgccgtggcc gccgccttgg cgcatctcca cggtctcacc gtcgcgacac cggcgctcgc
    13801 tgccgcgatc ggacgcggag cgcgctccgg catcggcatc ggcgcgttcg atctcggcgg
    13861 cttcatcgtc gatggcggcc tcaagagcgg cgccgaatac ccgccagtga ccgcgcatct
    13921 ggcatttccg gccgcgtggc gcattctgct gattctcgat cacggccgca gcgggttgca
    13981 tggcgacgcg gaacgcgcag ctttttccgc acttccgcct tttaccgaag cgctcgcggg
    14041 ccggctctgc cgtctcgtgc tgatgtcgct gctgccggga ttggccgaag aggatttcac
    14101 cgcggtatcg cacacgctgg gcgagatcca gtcgcggctc ggcgattatt tcagcccggc
    14161 gcagaacggc cgcttcagca gtcctgccgt ggcggacgca ttgacctggc tcggcgcgca
    14221 aggaatcacc gggaccggcc aatcctcctg gggaccgacc ggtttcgctc tgttcgaaag
    14281 cgccgccgaa gcaacgcgca tggccgaacg gttgagcgaa gagccgggct cgcgcgccct
    14341 cgaattgcag gtggtcgccg gacgcaatgc cggcgccgag atcgcggccc gccgcgccgt
    14401 caaggaggct cgtcgatgac tacgccacgc atccttcatt tcctcacgcc gctcgcgaat
    14461 gtgagtccct tcgacgtcaa catggcggtc gatgcgggct tcgtcgtcgc gagctacacg
    14521 aacatctcgc tcaaggaggt gacggcgctc acccaggacg cgatgttctc gcgctcgccg
    14581 gcggacgcgg ccaagacctg cctcttcatc ggcggacgcg atgcgctcca ggcgctcgac
    14641 atgctcgcgg ctgcgaagaa ggcgatgttc ccgcccttcg agatctcgct cttcgccgac
    14701 ccgtcgggcg ccttcacgac ggcggcagcg atggtggctt gcgtcgagcg tcagctcaaa
    14761 gcgcaagccg cgacgctcgc cggcgcggag gtggcggtgt tcggcgcgaa aggcgtggtg
    14821 ggcggtatcg tcggcgtcat cgccgccgaa gccggcgctc atgtcgtgct ggtcgctcat
    14881 gacaagagcg gcgtcgtgga agcgaaagcg gcggagttcg agcggcgctt cggacgctcc
    14941 tttgctgtcg ccgacggcag cagcgaggca ggccgccgcg gcgtgctgaa gaatgccgag
    15001 gtcgtcctcg cctgcggccg tgccggcctc cggatcctat cgcgcgcgga aatgagcgcc
    15061 gcacagcgac tcaaagtggc ggcggacatc aacgccgtgg cgccgcccgg catcgagggc
    15121 ctcgacgtga aggccaaagg gacgaagctg cccggaacgc ccggcatggg catcggtgcc
    15181 ttggcgatcg gcaacgtgaa attccagctg caacatgcgc tgctgaagcg tctcgacgag
    15241 gcgcagaagg cccagtgctt cgattttcgc gatgcctatc gcgtggcgct cgagcttgtc
    15301 cactgaggcg caaccggcgc tcgccgtcgc gctctcggcg cgttccctcg ccgcggcagc
    15361 gcgccgtgcc ggcgcatgcg tgatcgcgct cgacttgttt gccgatgagg atacggcggc
    15421 tgcagcacgc gcgtgccgca agctcgctcc ccttaccctc ccacaagggg agaggggagg
    15481 attgttcgca ttcgaccgtg aggcgctgct cgaggcggtc gaagaacacc gcggtaaagc
    15541 atcgggactc gtctatggtg ccggtttcga gcatgagccc gcgctgctgg ggatgcttga
    15601 atcgcgcatc gccattctcg gcaatcgagc ggaggtcatc ggcgcggtga aggatccctg
    15661 cggtttcgcc gcgctgctcg cacgccttgg cgtcccacat ccggagacgc ggctcgaagc
    15721 gccggcggag gggaattggc tcacgaagcg catcggcgga tcgggtggcg gacatataac
    15781 gcgtgcgtct gctggtgcca tcggcgcggg attttacgtg cagcggcagg tcgctggtcg
    15841 cgctgtatcg gcgctgttcg ttggcgacgg tcgacgggca agagttctcg gccacagcga
    15901 gcaatggacg tctcccaccg cagagatgcc gttccgctac ggaggctgtg t
//
LOCUS       DASYNS010000154         7176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1595771, whole genome shotgun sequence.
ACCESSION   DASYNS010000154 DASYNS010000000
VERSION     DASYNS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7176
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595771"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..839
                     /locus_tag="VGP50_04900"
     CDS             129..839
                     /locus_tag="VGP50_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8026743.1"
                     /translation="MTARLTGRLALVTGASRGIGAAVARRFAAEGAHVILVARTTGGL
                     EERDDEVRALGSEATLVPLDLRDFDKIDQMGAALYQRFGRLDVLVGNAGVLGSLSPMG
                     HFDPKTWSEVFDVNVTANWRLIRALDPLLRLSPSGRAVFTTCAAAREPLPYWGAYAAS
                     KAALATMVEVYAGEVTKTTLRVNLVDPGIVATKLRAHGFPGEDRAKLAQPDDVTELFV
                     TAAAADCSHHGEIMRDMN"
     gene            complement(1217..1714)
                     /locus_tag="VGP50_04905"
     CDS             complement(1217..1714)
                     /locus_tag="VGP50_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026744.1"
                     /translation="MSVRNILFTTSLAVLLPLAAIAPGHTAEGGGHHRHGGPRGDLGF
                     LTGVTLTDAQKTQIHQLLHASFTELKPLEQQRHALLAQISDQLASTASVSATQLTGLQ
                     QQAEQFGAQIEAQRLATALQIRALLTPDQLAQSAQVHQQLAALHAQMRSVVGQPGGTG
                     SPSAQ"
     gene            1973..2803
                     /locus_tag="VGP50_04910"
     CDS             1973..2803
                     /locus_tag="VGP50_04910"
                     /inference="COORDINATES: protein motif:HMM:NF020478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1849 family protein"
                     /protein_id="HEV8026745.1"
                     /translation="MSLLSARLLAAVAISFLFASMAVAADLASYRAHYSLTLASTRAA
                     SGVAGAGGAMVDEWVETCDGWTEQQHFYLHVLYEDEEPGADTFTTYYEFVSWEANDAQ
                     HYHFDMRQATSERPYHEVKGEAKAGGSGPAAGMIEFSRPQTTTLTLPPGAMFPLAYLR
                     LVIDGAKAGDRLVTHDVFDGSELDSAGQVTAVIGPRLAPDASPKEPLHANPLLDRPSW
                     HIAFAFFPSDHAAETPDYEESVRLLDNGITEDMVFNYGDYAIAAKLEQIEALPRPRCR
                     "
     gene            complement(2837..4288)
                     /locus_tag="VGP50_04915"
     CDS             complement(2837..4288)
                     /locus_tag="VGP50_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8026746.1"
                     /translation="MTGLIPRTLREAALDDVDARLSRAPEAIDGRFERACLLAELGRR
                     DAARDAYLELLARDPCHAGALNNLGTLLYAQGYRSAARTAYARAATQHPDDPMGHVNL
                     ANVLRETGDTAASRAHYDTALRLAPDHPEAHQGLAHLLADEGDAAGAERHRRRGFRGR
                     AVTAMPYHGDGQPIRLLLLVSAVGGNVPIQPLLDQGVFATVVIVAEFYDGDRALPAHD
                     LVFNAIGDADLCASGLEAAVGILARTASPIINPPGAVLMTGRAINARRLALLQDVIAP
                     AIVELPREAIAAADARAALGRMGFAFPLLLRAPGFHTGRYFLRVETADQLPAALAAIP
                     GRDLLVIQCLNAGSADGKTRKYRVMMVGGKIYPLHLAISDQWKVHYVTADMAERPEHR
                     AEEAQFLTDMESVVGPRGMAALERIRDALDLDYAGVDFALAPDGRVVVFEANTTMVVN
                     PPEPDQRWAYRRDAVERIHGAIRKLLMSKARGR"
     gene            complement(4460..5503)
                     /locus_tag="VGP50_04920"
     CDS             complement(4460..5503)
                     /locus_tag="VGP50_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8026747.1"
                     /translation="MAATTISEHVAKTTRHTSFYLACGPSDGPLIIFLHGWPELSISW
                     RHQLPAMAALGFRAVAPDLRGYGRSSIYDSHAAYAQQPIVEDMLELLAALGRERAVWV
                     GHDWGSPVVWNIASHHPERVTAIASLCVPYATIDRGLEAALPFVDRAIYPAEEFPMGQ
                     WDYMRFYEENFAKATATFEANPYNTAKALFRKGSPAGRGKPASTARIRKTGGWFGGAA
                     AAPDVPRDPDVVTEEDLSAYAAALARTGFFGPGSYYMNHAANAAYAARAPNDGRLDMP
                     VLFLAAAYDYICECVDSRLAEPMRRLCRDLTERVVPSGHWMAQEKPVAVNAALAHWLA
                     TRVPGAWPAAEMR"
     gene            complement(5522..6664)
                     /locus_tag="VGP50_04925"
     CDS             complement(5522..6664)
                     /locus_tag="VGP50_04925"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV8026748.1"
                     /translation="MQYRRISADCHLDLPWMPPDLFVSEAKHALKERMPFVADGPDGP
                     RWTARNGANFGLVNGVGPTGQKFVPGSHARVDIMASTGLYEDGRNGIRRVSDPHLRIK
                     DMDRDGIDAEVIYGILGAASRLDDRDAANEMLRIYNDWLRDFCRHYPEREIGLACLPY
                     GDIEAAVNEVHRAAKLGLRGLELSCSWDMEPMWHPCWEPLWQAVNEVRLPLHFHTFPA
                     MPLGKFEGQTGRTRRAAFFTVVSGFQMNLVNILAALMGAAVLERYPHIRVAFGESGIG
                     WIPYALDRMDFEWEDRFHDLGLKMKPSDYWRRQCKATFQFDRIGTRLIDEMGVETLMW
                     GCDYPHGDGVWPESEKYIAEQFAHLPPEMTRRITCENAGKFYGLIN"
     gene            complement(6862..>7176)
                     /locus_tag="VGP50_04930"
     CDS             complement(6862..>7176)
                     /locus_tag="VGP50_04930"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEV8026749.1"
                     /translation="EIFHLAPETIARAGEGDATAFAAASGRLLSGRKHLSAEQRPSMA
                     QDIAKGRRTEIDFLNGLVAEKGTELGIAVRANAALTDITRKIERGALDPHPRHLTELR
                     IN"
BASE COUNT         1231 a         2369 c         2412 g         1164 t
ORIGIN      
        1 cccaaattct gcgacgcctg cttcagcggc gattatccga tccctctcat cgaccacgag
       61 gccggcaaag tctcgacgca gctctcgctc ctcgccgaga cggcgtaggc ggctttcgtg
      121 cttcacccat gacggcccgc ctcacgggcc gcctcgccct cgtgaccggc gcttcgcggg
      181 gcatcggcgc ggcggtggcg cggcgcttcg cggccgaggg cgcgcatgtg atcctggtgg
      241 cgcgcaccac aggcggcctc gaagagcgcg acgacgaggt ccgcgcgctc ggcagtgaag
      301 cgacgctcgt gccgcttgat ctgcgcgatt tcgacaagat cgatcagatg ggcgcggcgc
      361 tctatcagcg cttcggccgc ctcgacgtgc tcgtcggcaa cgccggcgtg ctcggctcgc
      421 tgtcgcccat gggccatttc gatcccaaga cctggagcga agtcttcgac gtcaatgtca
      481 ccgcgaattg gcgtctgatc cgcgccctcg atccgctgct gaggttgtcg ccctccggcc
      541 gcgccgtctt caccacctgc gccgccgcgc gcgagcccct gccctattgg ggcgcctatg
      601 ccgccagcaa ggccgcgctc gcgacgatgg tcgaggtcta tgccggtgaa gtcaccaaga
      661 ccactcttcg cgtgaacctc gtcgatccgg gaatcgtcgc caccaagctc cgcgcccacg
      721 gctttcccgg cgaggaccgc gcaaagctcg cgcagcccga cgacgtgacg gagcttttcg
      781 tcacggcggc tgccgccgac tgttcacatc acggcgagat catgcgcgac atgaactgac
      841 acgcgcgccg tcgcggcgag gtttgaagcc ggcgcgaatg tgatcgcgcc atcgagggat
      901 gtcgcgcgcc atcgctacct attttgaaag agggcgtcga gcccgttcga ggtggcgata
      961 tcgtgacggc gaaacggata cgctgcaatt cacgcgagaa cggacacggt gcgcccgccg
     1021 caacggcagc gacgaccatg ctcgcggcgc aaccgtcgag ttgctcgacg ggggtcctgt
     1081 ggtctcggct ggcctatcgc tggctcgaag gagccgcgga aagggcgggc ggccggcccg
     1141 ctgccgaacc gcgccggaga gcctcgccgc gccgtcctct ctgatcagac ggaagcgatc
     1201 ccgcccgccc gttgagttat tgagccgacg gcgacccggt gccgccgggc tggccgacca
     1261 cgcttctcat ctgagcgtga agagccgcca gctgctgatg gacctgcgcc gactgcgcca
     1321 gctgatcggg cgtgaggagt gcacgaatct gcagagcggt ggcgagccgt tgcgcctcga
     1381 tctgcgcgcc gaactgttcc gcctgctgtt gcaggccggt gagctgcgtc gcgcttaccg
     1441 aggcggtgct ggccagctga tcggagatct gcgccaacaa tgcatggcgc tgctgctcga
     1501 gcggtttgag ctcggtgaag ctggcatgaa gcaactgatg gatctgagtc ttctgcgcgt
     1561 cggtgagcgt gaccccggtg agaaagccga gatcgccgcg cggcccgccg tgtcgatgat
     1621 gcccgccgcc ttcggcggta tggcccgggg cgatcgccgc cagcggcagg agcacggcga
     1681 ggctcgtcgt gaataatatg ttacgaacgg acatgatgat ctcctcgaca ggtgactctt
     1741 gcagccgacc aggaggttgt aggcaagata ccgctgataa atcccttcgc gcccgcgcga
     1801 tccatgcagt gaaactgctc aattcctcag tattcgtcag aacgaatgat cattcgcctc
     1861 tgcccgccgc ggcgagagcg gggacgcaag gcgcccgggc tgcctaaaag gattagagca
     1921 gccgcgagca acgcagaccc cttccattcc tcagacaggc tcccagtccc agatgagtct
     1981 tctgtccgcc cgactgctcg ccgccgtcgc tatctccttt ctgtttgcga gcatggcggt
     2041 cgccgcggac ctcgcctcct atcgggcgca ttacagcctg acccttgcgt cgacgcgggc
     2101 ggccagcggg gtcgcgggcg cgggcggagc gatggtcgac gaatgggtcg agacctgcga
     2161 cggctggacg gagcagcagc atttctatct gcacgtcctc tatgaggacg aggagcccgg
     2221 cgccgacacg ttcacgacct actatgaatt cgtgagctgg gaagcgaatg acgcgcagca
     2281 ctaccatttc gacatgcgcc aggccacgag cgagcgcccg taccacgagg tcaagggaga
     2341 ggcaaaggcg ggcggcagcg gcccggcggc cggcatgatc gagttcagcc ggccgcaaac
     2401 gacaaccctc accctgccgc cgggagcgat gtttcccttg gcctatctgc ggcttgtcat
     2461 cgacggggca aaggcgggag accggctggt cacgcacgac gtcttcgacg gctccgagct
     2521 ggacagcgcc ggccaggtca cggccgtcat cgggccgcgg ctggccccgg atgcgagccc
     2581 gaaggagcca ctgcacgcca accccctgct ggaccgcccc tcctggcaca tcgccttcgc
     2641 attcttccca tccgatcacg cggcggagac gccggactac gaggaaagcg tgcggcttct
     2701 cgacaatggc atcacggagg acatggtgtt caactatggc gactacgcca tcgccgccaa
     2761 gctggagcag atcgaagcct tgccgcggcc acgttgccga tagccgccgg ggatccggat
     2821 cccggccggg gctcgttcag cggccgcgcg ccttcgacat caacagcttg cgaatggcac
     2881 cgtggatccg ctccacggca tcgcggcgat aggcccatcg ctgatccggc tcgggcggat
     2941 tgacgaccat ggtggtattg gcctcgaaaa ccacgacgcg accgtcagga gccaaggcga
     3001 agtccacgcc agcgtagtcg aggtcgagag cgtcgcggat ccgttcgagc gccgccatgc
     3061 cccgcgggcc caccacactc tccatatcgg tgagaaattg ggcctcttcc gcccgatgct
     3121 cgggacgctc ggccatgtcg gcggtgacgt aatgcacctt ccactgatcg gagatggcga
     3181 ggtgcagggg atagatcttt ccgccgacca tcatcacgcg gtatttgcgc gtcttgccgt
     3241 cggcgcttcc ggcgttcagg cactgaatga cgaggaggtc gcgtcccgga atcgccgcca
     3301 gggcggccgg gagttggtcc gccgtctcga ctcgcagaaa atagcgtccg gtatgaaagc
     3361 ccggggcgcg cagcagaagc ggaaacgcga accccatccg gccaagcgcc gcgcgcgcat
     3421 ctgctgcggc gatggcttcg cgcggcagtt cgacgatagc cggcgcgatc acgtcctgga
     3481 gcaaggcaag gcggcgggca ttaatcgccc ggccggtcat gagcacggcg cccggtggat
     3541 tgatgatcgg cgacgcggtc cgcgccagaa tgccaacggc ggcctccaag cccgacgcac
     3601 agagatcggc gtcgccgatc gcgttgaaga ccaggtcgtg ggccggcagc gccctgtcgc
     3661 catcgtagaa ttcggcgacg atcaccacgg tcgcgaacac gccctgatcg agaagcggct
     3721 ggatcggcac attgccgccg acagcggaga cgagcagcag cagccggatg ggttgtccgt
     3781 cgccgtggta gggcatcgcc gtcacggcac ggccgcgaaa tccgcggcgg cgatgccgct
     3841 cggcgcccgc ggcatcccct tcatcggcga gcagatgggc aagcccctga tgcgcctccg
     3901 gatggtcggg cgccaagcgc agcgccgtgt cgtaatgtgc acgcgaagcc gcggtgtcgc
     3961 cggtctcgcg caggacattc gccaggttga catgacccat cggatcgtcc ggatgctgcg
     4021 tcgcggcccg cgcataggcg gtacgggcgg cgctccggta tccttgggca tagaggagcg
     4081 ttcccagatt gttgagtgcg ccggcatggc agggatcgcg cgccagcaat tcgagataag
     4141 cgtccctcgc cgcgtcgcgc cgacccaact ccgccaacag acaggcccgc tcgaaccgcc
     4201 catcgatcgc ttccggcgcg cgcgacagac gcgcatcgac atcgtcgagc gcggcctcgc
     4261 gcagcgtccg tgggatcaat ccagtcatgt cactcgccac gacgcgtcgc gccgcgcggc
     4321 ccgccgggcc cgcgcgacag ccgacgcatc acttgtgatg gtcgggagat tggcgagcaa
     4381 ccctgtacac ggaagtggct aacgaaggct ttaccatatt ccgcttctgg cgcccggtcg
     4441 gcgctgcgcc gagccggttc tatcgcatct ccgccgcggg ccaggcgccg gggacgcgcg
     4501 tcgcgagcca atgggcgagc gccgcgttga cggcgaccgg cttctcttgc gccatccaat
     4561 ggcccgacgg cacgacgcgt tcggtgagat cgcggcagag gcggcgcatg ggctcggcca
     4621 gccgcgagtc gacgcattcg cagatgtagt cataggcggc ggcgagaaac agcacgggca
     4681 tgtcgaggcg gccgtcattc ggggcgcgcg cggcataagc ggcgttggcc gcgtggttca
     4741 tgtagtagga accggggccg aaaaatccgg tgcgcgccag cgccgcggca taggcgctga
     4801 gatcctcctc ggtcaccaca tccggatcgc gcggcacatc cggcgccgcc gcagcgccgc
     4861 cgaaccagcc gccagttttg cgaatgcgcg ccgtcgatgc gggctttccg cgccccgcgg
     4921 ggctgccctt gcggaagagc gccttcgccg tgttgtaggg attggcctcg aaggtcgccg
     4981 tcgccttcgc gaagttctct tcgtagaacc gcatgtaatc ccactgcccc atgggaaatt
     5041 cctcggccgg ataaatcgcg cgatcgacga acgggagcgc ggcctccagg ccgcggtcga
     5101 tggtcgcata agggacgcag agactggcga tggcggtcac gcgctcggga tgatggctgg
     5161 cgatgttcca gaccaccggg ctgccccaat catgcccgac ccagaccgcg cgctcgcggc
     5221 cgagcgccgc caggagctcg agcatgtcct cgacgatggg ctgctgggcg taggccgcgt
     5281 ggctgtcgta gatgctcgag cggccatagc cgcgcagatc cggtgccacg gcgcgaaagc
     5341 cgagcgccgc catggctggc agctggtgac gccagctgat cgagagctcg ggccagccat
     5401 gcaagaagat gatcagcggt ccgtcgctcg gcccgcacgc aagatagaag ctcgtgtggc
     5461 gcgtcgtttt cgccacatgc tcgctgatcg tcgtcgccgc caagtccatt ctcccgccgg
     5521 ctcaattgat cagcccgtag aactttccgg cgttctcgca ggtgatcctg cgggtcattt
     5581 caggcggcaa gtgggcgaat tgctcggcga tgtatttctc cgactccggc cagacgccgt
     5641 cgccatgcgg atagtcgcag ccccacatca gcgtctcgac gcccatctcg tcgatgagcc
     5701 tggtgccgat gcggtcgaac tggaaggttg ccttgcattg ccggcgccaa tagtcgctgg
     5761 gcttcatctt gaggccgaga tcgtggaagc ggtcctccca ttcgaaatcc atgcggtcga
     5821 gcgcataagg aatccagccg atgccgcttt cgccgaaagc gacgcggatg tggggatagc
     5881 gctcgagcac ggccgcgccc atcaaggccg cgaggatgtt gacgaggttc atctggaacc
     5941 cggatacgac ggtgaagaaa gcggcgcgcc gcgtccggcc ggtctgaccc tcgaacttgc
     6001 cgagcggcat ggccgggaag gtgtggaagt ggagcggcag ccgcacctcg ttcaccgcct
     6061 gccagagcgg ctcccagcag ggatgccaca tcggctccat gtcccaggag caggagagct
     6121 cgaggccgcg caaaccgagc ttggccgcgc gatggacttc gttcacggcg gcctcgatgt
     6181 cgccataggg gagacaggcg aggccgatct cgcgctcggg ataatgccgg cagaaatccc
     6241 tgagccaatc attgtagatg cgcagcatct cgttggcggc gtcgcggtcg tcgagacggc
     6301 tggcggcgcc caggatgccg tagatcacct cggcatcgat gccgtcgcgg tccatgtcct
     6361 tgatgcgcag atgcggatcg gagacgcggc ggataccgtt gcgaccatcc tcgtagagtc
     6421 cggtcgacgc catgatatcg acgcgcgcat ggctgcccgg aacgaatttc tggccggtcg
     6481 ggccgacgcc gttgacgagc ccgaaattgg cgccgttccg cgccgtccaa cgcggaccgt
     6541 cgggaccgtc ggcgacgaaa ggcatgcgct ccttcaaggc gtgcttcgcc tcggacacga
     6601 agagatcggg cggcatccac ggcaagtcga gatgacaatc ggcggaaatg cggcggtact
     6661 gcatgagatt caccacagag gcagcgaggc agtgagaaga gattagagca gatctcgagc
     6721 cggtggaatc ggcactttcc caacatcgtc atccccgcga aagcggggac ccaggggcca
     6781 ccagcgcgtc gcttgccctg gatccccgct ttcgcgggga tgacgacgag gaggatattc
     6841 cagccaaccg gggagctgct ctcagttgat cctcaattcc gtgaggtggc gcggatgcgg
     6901 gtcgagcgcg ccgcgctcga tcttgcgcgt gatgtcggtg agggccgcat tggcgcggac
     6961 ggcgatgccg agctcagtcc ccttctcggc caccaggccg ttcaggaaat cgatctcggt
     7021 gcgccggccc ttggcgatgt cctgggccat ggagggacgc tgctcggcgg agagatgctt
     7081 ccgcccggag agcagccggc cggaggcggc ggcgaaagcg gtcgcgtcgc cctcgccggc
     7141 gcgcgcaatc gtctcgggcg cgagatggaa gatctc
//
LOCUS       DASYNS010000155         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_814167, whole genome shotgun sequence.
ACCESSION   DASYNS010000155 DASYNS010000000
VERSION     DASYNS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814167"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VGP50_04935"
     CDS             <1..277
                     /locus_tag="VGP50_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV8026750.1"
                     /translation="WAALAKAAPTLVFYMALKHLDIIARRLMAAGRPADEPVAVISKA
                     TTPAQRVLVTTLGRVAAETAAAGIEPPAIVAVGAAVRLRASLAWLET"
     gene            complement(282..626)
                     /locus_tag="VGP50_04940"
     CDS             complement(282..626)
                     /locus_tag="VGP50_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV8026751.1"
                     /translation="MPAPLPYYAALFAGILLGVLGQVALKTGAERSSGMVAQFLDPFT
                     IGGFAVYALAAIFYIIAIKRIPISLAFPSVSLSYVAVAIIAHLVWGEPLGLPQIGGIA
                     LITGGILLLHQS"
     gene            complement(626..1621)
                     /locus_tag="VGP50_04945"
     CDS             complement(626..1621)
                     /locus_tag="VGP50_04945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV8026752.1"
                     /translation="MTPSLALSIVIPVYNGAQSIGEVVAALAGLSVPGGHEIVLVNDC
                     SPDNSLGVCRDLLAGTRVPMTLVNLSRNYGEHNAVMAGLRHARGAHVITMDDDLQNPP
                     AEVLRLLEYAQSSGKEVIYTRYASKEHTGWRNLGSRFTNRVADELLDKPKGLYLSSFR
                     CMSAFVAEEITAYDGPFPYVDGLILQVTQSIGSLEVQHLPRAYGRSNYTIRRLVRLWL
                     NMFVNFSVMPLRLATAFGFALSLLGLAGTLWVIMEALLVETPPGWASLAVAVLLLSGV
                     QLTMLGLIGEYLGRLYLTANRKPQSVIKDVTRNGAAEPLLRQPDDRRGTLVMRHG"
     gene            complement(1618..>2080)
                     /locus_tag="VGP50_04950"
     CDS             complement(1618..>2080)
                     /locus_tag="VGP50_04950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8026753.1"
                     /translation="DRLPLGEGTLDAVLSADVLCHRGVDAAAGLTEIRRCLRPGGVLV
                     LNLPAYAWLWSAHDAAVDNVRRFGRAELEQLLAAAGFAAVRAFYWNSFLFPLMVLRRK
                     VWRHAGSDVGLLPAPVERLFALIMALESRLRRAGLALPFGGSILALAVKPS"
BASE COUNT          370 a          671 c          758 g          281 t
ORIGIN      
        1 ttgggcggcg ctcgccaaag ccgcgccgac gctggtgttc tacatggcgc tgaagcatct
       61 cgacatcatc gcgcgccgcc tgatggcggc cggacgtccc gccgatgagc ccgtcgcggt
      121 gatcagcaag gcgacgacgc cggcgcagcg cgtgctggtg acgacgctcg gccgcgtcgc
      181 cgcggaaacc gcggccgccg gcatcgagcc gccggccatc gtcgcggtcg gcgccgccgt
      241 gcggctgcgc gcgagcctcg cgtggctgga gacgtgaccg gctacgactg gtggagcagg
      301 agaatcccgc cggtgatgag ggcgatgcca ccgatctgcg gcagtcccag aggctcgccc
      361 cagacgagat gcgcgatgat cgccaccgcc acgtaactca gcgacacgct gggaaaggcg
      421 agcgagatcg ggatgcgctt gatggcgatg atgtagaaga tggcggcgag cgcatagacg
      481 gcaaagccgc cgatggtgaa gggatcgagg aactgcgcga ccatgccgga ggagcgctcg
      541 gcgccggtct tgagcgcgac ctggccgagc accccgagca ggatgccggc gaagagcgcc
      601 gcgtaatagg gcagcggcgc cggcatcagc cgtgccgcat gacgagcgtg ccgcgccggt
      661 cgtcgggctg gcgcagcagc ggctcggccg cgccgttgcg cgtcacgtcc ttgatcaccg
      721 attgcggctt gcggttcgcg gtgagataga gccgcccgag atattcgccg atgaggccga
      781 gcatggtgag ctgcacgccc gagagcagca gcaccgccac cgccagcgag gcccagccgg
      841 gcggcgtctc caccagcagc gcttccatga tcacccagag cgtgccggcg agaccgagga
      901 ggctcagcgc gaatccgaag gcggtggcga ggcggagcgg catcaccgag aagttcacga
      961 acatgttgag ccagagccgc accagccggc ggatggtgta gttgctgcgg ccataagcac
     1021 gcggcagatg ctgcacctcg aggctgccga tcgactgcgt gacctggagg atcaagccgt
     1081 cgacgtaagg gaaaggcccg tcataggcgg tgatctcttc ggcgacgaag gcgctcatgc
     1141 agcggaagct cgagagatag aggcccttgg gcttgtcgag cagctcatcg gcgacgcggt
     1201 tggtgaagcg gctgccgaga ttgcgccagc ccgtatgctc cttgctggca tagcgggtat
     1261 agatcacctc cttgccggag ctctgcgcgt attcgaggag gcgcagcacc tcggcgggcg
     1321 ggttctgcag atcgtcgtcc atggtgatga catgcgcgcc ccgggcatgg cggagccccg
     1381 ccatgacggc attgtgctcg ccataattgc gcgacaggtt gaccagcgtc atcggcacgc
     1441 gggtgccggc gaggagatcg cggcacacgc cgaggctgtt gtcggggctg cagtcgttga
     1501 cgagcacgat ctcgtggccg ccgggcacgc tgaggccggc cagcgccgcc accacctcgc
     1561 cgatgctttg cgcgccgtta tagaccggga tgacgatgct gagcgccagc gaaggtgtca
     1621 tgagggtttc accgctaggg ccaggatgga gccgccgaag ggcaaggcca atcccgcccg
     1681 ccgcaaccgg ctctcgagcg ccatgatgag agcgaagagg cgctcgaccg gtgccgggag
     1741 caaccccacg tcgctcccag cgtggcgcca taccttgcgc cgtaacacca tcaggggaaa
     1801 gagaaaactg ttccagtaaa aggcccggac cgcggcgaag ccggcggcgg cgaggagctg
     1861 ctcgagctcg gcgcggccga agcggcgcac attgtcgacg gcggcgtcat gggcggacca
     1921 gagccaggca taggccggca gattgaggac gaggacgccg cccggccgca ggcagcgccg
     1981 gatctccgtc aatcccgccg ccgcgtccac gccgcgatgg cagagcacgt cggcgctgag
     2041 cacggcgtcg agcgtgccct cgcccagcgg cagccggtcg
//
LOCUS       DASYNS010000156         5174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4404174, whole genome shotgun sequence.
ACCESSION   DASYNS010000156 DASYNS010000000
VERSION     DASYNS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4404174"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="VGP50_04955"
     CDS             <1..1021
                     /locus_tag="VGP50_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEV8026754.1"
                     /translation="RRGAVALKPPGEAGGCLALDKLFALNPALAAIHPYWGKGELLFV
                     HASGNGYRTRSHFDAQDLMESGLSAKGTMSDGWLNRALALLQKGDHRLGLAVGGAVPL
                     VLRGPVPVASWEPAALQDTSPEFMTALAQLYRGDPLLGPALGEGLKGQNFSESVLGDD
                     MAGGKRGFGPKSFAPLAAAAGKLLAAPDGPRLAVLEMGGWDTHVNQGTESGRLAENLR
                     GLADGLDALAQALGPNWSQTVLVAVTEFGRTVAANGNNGTDHGTASVLFVMGGAVKGG
                     RIAGDWPGLEQLQDDRDLRVATDSRRVMKALLRDHLGLDAATLDRKVFPDTTALTPVS
                     GLLRA"
     gene            1180..2568
                     /locus_tag="VGP50_04960"
     CDS             1180..2568
                     /locus_tag="VGP50_04960"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEV8026755.1"
                     /translation="MQGDRRQRLAFPMRSLASIAAVVVALSLFLAAIFFLNLNLVRLK
                     ESFGWVQHTDEVLLQLAAIEATVIEAESAERGYLLTGDQRYRDPYDRAEAGLDGQIDA
                     LAQLVADNPGQRQRVEELRPVIAARMAEFKQAIDLGPSRLGDALAVVQAAQRQPLTQA
                     ARERLEQLRQTEIGLLGERQRRVADSAILSTALAAGATVLSLASAGLGLFFFLRQRNS
                     YRIRELQTELLHVARLNTMGQTASVIAHEVKQPLAATAAYLQGMRRMIDTAGLAASGR
                     VTEALQKALAQVNRAVDILGRLRRFVERHDTQRQAEPIDGVIDEAVALMAIDSGDVAL
                     RRRVGPNLPAVVIDRIEVQQVLINLMRNAVEAMGESPRRELTLSAEAAERMVRVGVAD
                     TGPGLPPLVAERLFQPFVSTKEDGMGVGLSICRTIVESQGGRIWATANPEGGTTFHFT
                     LPAAEAGAERAA"
     gene            2661..3041
                     /locus_tag="VGP50_04965"
     CDS             2661..3041
                     /locus_tag="VGP50_04965"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEV8026756.1"
                     /translation="MPLSRISLRRGKTAAYRRAVMDQVYEALRETFDVPEDDRFMLVT
                     EHDEDSFSFGRRYLGIERTDDLIIIQITVSNTRGIEQKKRLYKRIAERLADRPGVRPQ
                     DVFVNLLEVAKENWSFGNGEAQYA"
     gene            complement(3054..3800)
                     /locus_tag="VGP50_04970"
     CDS             complement(3054..3800)
                     /locus_tag="VGP50_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEV8026757.1"
                     /translation="MLLHLQTWNEVDAYLERARGIIVPIGSTEQHGPNGLIGTDAICA
                     EAIAAGVGTAAGALVGPTISVGNAQHHLAFAGTITLRPSTLMAVIGDYVFSLARHGFE
                     RFYFINGHGGNIATVGAAFAEIYAQASLRPADNARPLRCALKNWWQTPDVQKLTKEIF
                     GDSEGSHATPSEVSVTQHVHPEAIKRRAMGPPARHARNFTDAADYRRLFPDGRIGSDP
                     SLASPEHGARLVEAAVRDIAEDYRSFMARD"
     gene            3891..4775
                     /gene="panC"
                     /locus_tag="VGP50_04975"
     CDS             3891..4775
                     /gene="panC"
                     /locus_tag="VGP50_04975"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEV8026758.1"
                     /translation="MKRATTERIGKLPVARRVGDLRRSIAGWRRAGETVALVPTMGAL
                     HAGHLALVTRARELCDRAVASIFVNPAQFGPNEDFARYPRDEAGDAAKLVQARCDLLY
                     APAVEEIYPPGFSTTVTAGAIAEGLCGRFRPGHFAGVATIVTKLLVEAQADVACFGEK
                     DYQQLQVIRRVAHDLDIPVRIEGVATVREADGLALSSRNAYLTPEERVIAPALQRTLR
                     DVARRIESGETGIDTAVEAGLAELARAGFSKIDYLEACDADTLAPLTRPDRPGRILAA
                     AWLGRTRLIDNVPVPQQR"
     gene            complement(4807..>5174)
                     /locus_tag="VGP50_04980"
     CDS             complement(4807..>5174)
                     /locus_tag="VGP50_04980"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEV8026759.1"
                     /translation="KRSETQVTAAFDPTQALSGIEPIFQVGNKWLENWMAVSSELLEF
                     SRARLDRSLEIGKAIARSGSIDEAMELQADYARSTVREYFTEAGKIADLSTRALLDGI
                     VKLQPAIRETARRVTSATA"
BASE COUNT          792 a         1747 c         1794 g          841 t
ORIGIN      
        1 ccgccgcggc gccgtcgcct tgaagccgcc cggcgaagcc ggcggctgtc tcgcgctcga
       61 caagctcttc gcgctgaacc cggcgctggc ggcgatccac ccttactggg gtaaaggcga
      121 gctgcttttc gtccatgcct ccggcaatgg ctaccgcacg cgctcgcatt tcgacgcgca
      181 ggatctgatg gagagcgggc tctcggccaa aggcaccatg agcgatggct ggctcaaccg
      241 cgcgctggcg ctgctgcaga agggcgacca tcgcttggga ctcgcggtcg gcggcgccgt
      301 gccgctcgtg ctgcgcgggc cggtaccggt cgcatcctgg gagccggcgg cgctgcagga
      361 cacctcgccc gaattcatga cggcgctggc gcagctctat cgcggcgatc cgctgctcgg
      421 gccggcgctc ggcgagggat tgaaggggca gaatttttcc gaatccgtgc tcggcgacga
      481 catggcgggc ggcaagcgcg gcttcggccc caagagtttc gcgccgctcg ccgcggccgc
      541 cggcaagctc ctcgcggcgc ccgacgggcc gcggctcgcg gtgctcgaga tgggcggctg
      601 ggatacgcat gtgaaccaag gcacggagag cgggcggctc gccgagaatc tccgcggctt
      661 ggccgacggc ctcgacgcgc tggcacaggc gctgggcccg aactggtcgc agaccgtcct
      721 ggtcgcggtc accgagttcg gccgcacggt cgcggccaat ggcaacaacg ggaccgacca
      781 tggcacggca tccgtgctgt tcgtcatggg gggcgcggtc aagggcggtc gcatcgccgg
      841 cgattggccc ggcctcgagc agcttcagga cgatcgcgat ctccgcgttg ccaccgattc
      901 gcgccgcgtc atgaaagcgc tgctgcgcga tcacctcggc ctcgacgccg cgaccctcga
      961 tcgcaaggtg tttccggaca cgaccgcatt gacaccggtg agcgggctgc tgcgcgctta
     1021 agccgttttc gtcgataggc acatttccat cgcgctgcct ccgacgcgac agcgcgatac
     1081 cgatcgcctt tcgggtgcga cacggccttg catctcccga ggtccttccg ccatcctccg
     1141 ccagggcgat cgcgcatcct tgaccgtggc gggccgggca tgcagggaga caggcgtcag
     1201 cggctggcgt ttccgatgcg ctcgctggcg agcatcgccg ccgtcgtcgt cgcgctttct
     1261 ctttttctgg cggcgatctt ctttctcaat ctcaatttgg ttcgcctcaa ggagagcttc
     1321 ggctgggtgc agcatacgga cgaggtcctg ctgcagctcg ccgccatcga ggccacggtg
     1381 atcgaagccg aatcggccga gcgtggctat ctcctcaccg gcgatcagcg ctatcgcgat
     1441 ccctatgacc gcgcggaagc cgggctcgac gggcagatcg atgcgctggc gcagctcgtc
     1501 gccgacaacc ccggccagcg acagcgcgtc gaggagctgc gccccgtcat cgccgcacgc
     1561 atggccgagt tcaagcaagc gatcgacctc ggtccctccc ggctcggcga cgccttggcc
     1621 gtcgttcagg cggcgcagcg gcagcccctg acgcaagccg cgcgcgagcg tctcgagcag
     1681 ttgcggcaga ccgagatcgg cctgctcggc gagcggcaac ggcgtgtcgc ggattcggca
     1741 attctgtcca ccgcccttgc cgccggcgcg acggtgctca gcttggcgag cgccgggctc
     1801 ggactctttt tcttcctgcg ccagaggaac agttaccgga tccgcgaatt gcagacggag
     1861 ctgctgcacg tcgcccggct caacaccatg ggccagacgg cgtcggtgat cgcgcacgag
     1921 gtcaaacaac cgctggcagc gaccgccgct tatcttcaag gcatgcggcg catgatcgac
     1981 acggccggcc tcgcggcgtc cggccgggtc accgaggcgc tgcaaaaggc gttggcgcag
     2041 gtgaatcgcg ccgtcgacat cctcgggcgc ctgcggcgct tcgtcgagcg gcacgacacg
     2101 cagcgccaag ccgagccgat cgatggggtg atcgacgagg cggtcgcgct gatggccatc
     2161 gatagcggcg acgtcgcgct tcgccgacgc gtcgggccca acttgcccgc cgtggtgatc
     2221 gaccggatcg aggtgcagca agtgctgatc aacctgatgc gcaatgccgt cgaagcgatg
     2281 ggcgagagcc cgcggcgcga gctcaccctc tcggccgagg cggcagagcg catggtccgc
     2341 gtcggcgtcg ccgacaccgg gccgggtctg ccgccgctcg tggcggaacg gctgttccag
     2401 cccttcgtct cgaccaagga ggacggcatg ggcgtcggcc tctcgatctg ccgcaccatt
     2461 gtcgagagcc aagggggccg gatctgggcg acggccaatc ccgagggcgg cacgaccttc
     2521 catttcacct tgcccgcggc cgaagccggc gccgagcgcg ccgcatagcg agcgcggcag
     2581 ctattccatc aacgcagcgc ccgccgcgag ccggagccgc tatcctgcgc gattggagca
     2641 agcagacgag ggaggcattc gtgccgttgt cacggatatc gttacggcgt gggaagactg
     2701 ccgcttatcg gcgcgccgtc atggaccagg tgtatgaagc gctgcgcgag acgttcgacg
     2761 tgccggagga cgatcgtttc atgctcgtga ccgagcatga cgaggacagc ttcagcttcg
     2821 gccgccgcta tctcggaatc gagcgcaccg acgatctgat catcatccag atcaccgtca
     2881 gcaacacgcg cggcatcgag cagaagaagc gcctctacaa gcgcattgcc gagcgcctcg
     2941 ccgaccggcc gggcgtccgt ccgcaagacg tcttcgtgaa tcttctcgaa gtcgcgaagg
     3001 agaactggtc cttcggcaat ggcgaggcgc aatacgcgta ggccggttgc cccctaatcc
     3061 cgcgccataa aactccggta gtcctcggcg atgtcgcgca cggcggcctc gacgagccgc
     3121 gcgccatgct cgggcgatgc gagcgaggga tcggagccga tgcggccgtc cggaaaaagc
     3181 cgccgatagt cggcggcgtc ggtgaagttg cgcgcgtggc gcgccggcgg tcccatcgcg
     3241 cggcgcttga tcgcttcggg atgcacatgc tgcgtcacgg agacctcgct gggtgtcgcg
     3301 tggctgcctt cgctgtcgcc gaagatctcc ttggtgagtt tctggacgtc cggcgtctgc
     3361 caccagttct tgagggcgca gcgcaaaggg cgcgcgttat cggccggtcg gagcgaggct
     3421 tgtgcgtaga tctcggcgaa ggcagcgccg accgtcgcga tgttgccgcc atggccgttg
     3481 atgaaataga agcgctcgaa gccgtggcgc gccagcgaga agacgtagtc gccgatcacg
     3541 gccatgaggg tcgagggccg gagcgtgatc gtgccggcaa aggccaggtg atgctgggca
     3601 ttgccgacgc tgatggtggg accgaccagc gcccctgccg cggtaccaac gccggccgcg
     3661 atcgcctcgg cgcagatcgc atcggtgccg atgaggccgt tcggcccgtg ctgttcggtc
     3721 gagccgatcg gcacgatgat gccgcgggcc cgctcgagat aggcgtcgac ctcgttccag
     3781 gtctggagat ggagcagcac gggatcgttt tcctcctcgc ccgcaccata gcacgcggcc
     3841 ggagatagcg cttgccgctg gcgtgccggc ggtctacacc gcgccgcagc atgaaacggg
     3901 caacgacgga gcggatcggc aagctgcccg tggcgcggcg cgtgggcgat ctgcggcgct
     3961 cgatcgccgg ctggcgccgc gccggcgaga cggtggcgct ggtgccgacc atgggcgcgc
     4021 tccacgccgg gcatctggcg ctggtgacgc gcgcgcgcga gctctgcgac cgcgcggttg
     4081 ccagcatctt cgtcaacccg gcgcagttcg gccccaacga ggacttcgcc cgctatccgc
     4141 gcgacgaggc gggcgatgcg gcgaagctcg tgcaggcccg ttgcgatctc ctctatgcgc
     4201 cggcggtgga agagatctat cccccgggct tctcgacgac cgtaacggcg ggtgccatcg
     4261 ccgaagggct atgcgggcgc ttccggcccg ggcatttcgc cggcgtggcg acgatcgtga
     4321 cgaagctgct cgtcgaggca caagccgacg tcgcctgctt cggcgagaag gactaccagc
     4381 agctccaggt gatccgccgt gttgcgcacg atctcgacat ccccgtgcgg atcgagggcg
     4441 tggcgacggt gcgcgaggcg gacgggctcg cgctctcctc gcgcaacgcc tatctcacgc
     4501 ccgaggagcg ggtgatcgca ccggcgctgc agcgcaccct ccgcgacgtc gcgcggcgca
     4561 tcgaaagcgg cgagaccggc atcgacaccg ccgtcgaagc cgggcttgcg gagctggcgc
     4621 gcgccggctt cagcaagatc gactatctcg aggcctgcga tgcagacacg cttgcgccgc
     4681 tgacgcgtcc cgatcgtccc ggccgtattt tagcggccgc gtggctcggc aggacgcgtc
     4741 tcatcgacaa tgtgccagtg ccgcagcaac gctgacgcga tcggccgcgg ggtcgcctgt
     4801 cgtatctcag gccgtggccg aggttacgcg gcgggcggtc tcgcggatgg ccggttgcag
     4861 cttcacaatg ccgtcgagga gcgcgcgcgt gctgaggtcg gcgatcttcc cggcctccgt
     4921 gaagtattcg cgcaccgtcg agcgcgcata atctgcctgc agctccatcg cttcatcgat
     4981 gctgcccgag cgtgcgatgg ccttgccgat ctcgagactg cggtcgaggc gagcacggct
     5041 gaattcgaga agctcggagc tgacggccat ccagttctcg agccatttgt tgccgacctg
     5101 gaagatcggc tcgatcccgc tcagcgcctg cgttggatcg aacgccgcgg tgacctgcgt
     5161 ctcgctccgc ttcg
//
LOCUS       DASYNS010000157         8864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1127433, whole genome shotgun sequence.
ACCESSION   DASYNS010000157 DASYNS010000000
VERSION     DASYNS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127433"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1265)
                     /locus_tag="VGP50_04985"
     CDS             complement(93..1265)
                     /locus_tag="VGP50_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEV8026760.1"
                     /translation="MPTNRYAMLDFDLGPEIDQLRDSVRAFADDEIAPLAAEIDRSDK
                     FPRQLWPKMGELGLHGITVEEEYGGTGMGYLAHVVAMEEVSRGSASVGLSYGAHSNLC
                     VNQIRRNGSEAQKHKYLPKLMSGEHVGGLAMSEPSAGSDVVGMRTRADKRGGRYVLNG
                     SKMWITNGPEAETLVVYAKTDANAGPRGITAFLIEKGMKGFAPAQKLDKLGMRGSPTS
                     ELVFTDCEVPEENVLGKVNDGVRVLMSGLDYERAVLAAGPLGIMQSCLETVIPYVHER
                     KQFGQAIGEFQLMQGKLADMYTTMNACKAYVYAVARACDRGQATRKDAAGAILYASEK
                     ATLMALEAIQALGGNGYINDYATGRLLRDAKLYEIGAGTSEIRRMLIGRELFTETA"
     gene            1401..1874
                     /locus_tag="VGP50_04990"
     CDS             1401..1874
                     /locus_tag="VGP50_04990"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEV8026761.1"
                     /translation="MNSRKVVLPALAIAVLVGGAGLGSLTTQQAAAQATTAPQPAEQH
                     ERHHFSPTRHVEGRIAYLKAELKITDAEEQQFAPVAQAMRENAKTMAQAMEQRHGMRD
                     QPQTAVQRLEARAQFAGLRAQADQRFVDAFKPLYASLSADQKKAADELLTRHGHR"
     gene            1972..2223
                     /locus_tag="VGP50_04995"
     CDS             1972..2223
                     /locus_tag="VGP50_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEV8026762.1"
                     /translation="MNVTRRTALTTAAMSGVALVAGVSSAQAEPHPKIKQAIHALESA
                     RDDMKHAAHDFGGHREDALRACDEALRQLHLALDWANHH"
     gene            complement(2248..4530)
                     /locus_tag="VGP50_05000"
     CDS             complement(2248..4530)
                     /locus_tag="VGP50_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEV8026763.1"
                     /translation="MAEDSRDDALEYHRLPTPGKIGIVATKPLATQRDLALAYSPGVA
                     AASLAIAADPQQASSLTVRANLVGVVTNGTAVLGLGAIGPLAAKPVMEGKAVLFQKFA
                     GIDVFDIEIDERDPDKLVEVIAALEPTFGGINLEDIKAPECFAIEAKLRQRLKIPVFH
                     DDQHGTSIIVGAAIRNGLRLIGKSLAEVRLVSSGAGAAAIACLDLLVDMGLPLENIIV
                     TDKYGVVWRGRKEEMDPRKDRYAKETNARTLGEVIAGADIFLGLSAPDVLTPEMVAKM
                     AAQPLILALANPDPEIRPEAAKAVRPDAIIATGRTDYPNQVNNVLCFPFIFRGALDVG
                     ATVINEAMKLACVAALADLALAEPSDVVAAAYGDASLTFGPDYLIPKPFDPRLILEIA
                     PAVAKAAMESGVATRPIGDLDAYRQRLSQFVFRSGLLMKPVFDRARGEPKRLVYAEGE
                     DERVLRAVQQVVDDGLARPIIIGRRDVVLRRIEKLNLRLALGKTVTLVDPQDDPRYHE
                     YWTLYHSLMERKGVSPDFARGLVRTRATVIAALMVRRGEADAMLCGAIGQYHRHLRHL
                     IDVLGLVPGASAAAALSVLITPKGTFFLCDTHVVDDPSAEQLAEMTLRAADAVRRFGV
                     PPKVALLSHSDFGSADSPSARKMRQALALLRQRAPALEVEGEMHADAALSEEIRRRIF
                     PNALLKGVANLLIMPNLDAANITFNSLRVLADGQPVGPILLGVNGAAHIVAPSITVRG
                     LINMTAIAVYDAQLSVTTRP"
     gene            complement(4632..4718)
                     /locus_tag="VGP50_05005"
     tRNA            complement(4632..4718)
                     /locus_tag="VGP50_05005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4682..4684),aa:Leu,seq:gag)
     gene            4794..5444
                     /gene="lipB"
                     /locus_tag="VGP50_05010"
     CDS             4794..5444
                     /gene="lipB"
                     /locus_tag="VGP50_05010"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEV8026764.1"
                     /translation="MPGTSTASIEWRVSEAPVAYEAAVAAMEERVAAIRAGSAPELVW
                     LLKHPPLYTAGTSANERDLVEPGLFPVHRTGRGGQFTYHGPGQRVGYVMLDLQRRGPD
                     LRCYVRDLEEWLIRTLRSFGVAGERRPGRVGIWIAEPGGRERKIAAIGIRVRRWVSFH
                     GVSLNVDPDLSHYRGIVACGIQEHGVTSLADQGVRASMREVDDAMRQAFASVFGAE"
     gene            complement(5578..6033)
                     /locus_tag="VGP50_05015"
     CDS             complement(5578..6033)
                     /locus_tag="VGP50_05015"
                     /inference="COORDINATES: protein motif:HMM:NF045398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026765.1"
                     /translation="MTKNYIVSGHGGCFKGEQWWVPDGVQIHFYCMKDRVLGDNKGYT
                     ILDKLLLNVDVYPVWVAQPKTALSAYSCWAYPGIAPASGVFRRKSGEMVMSLAGTSGK
                     KPVALGHIAHVLAKQRAEAGKWTMIHWLACTTESTGSSANVDYQAPTRV"
     gene            6410..7132
                     /locus_tag="VGP50_05020"
     CDS             6410..7132
                     /locus_tag="VGP50_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8026766.1"
                     /translation="MQVIAVASQKGGSGKTTLAGHLAVQADRTGCGPVALVDTDPQGS
                     LSEWWNARTAETPLFARTSAGRLAQDIERMRELGIKLLVVDTPPAIEATITEVIRLAD
                     LVVIPARPSPHDLRAAGATVELIERLGKPLIFVVNAATPRARITSEAVMALSQHGTLA
                     PVILHHRTGFAAAMIDGRTVMEVPGEARSAEEIELLWQYLAKRLNRDFGTMLPAALLS
                     SAPKDGNPELGDQDGPYEQGAS"
     gene            7129..7518
                     /locus_tag="VGP50_05025"
     CDS             7129..7518
                     /locus_tag="VGP50_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026767.1"
                     /translation="MSSSSTKVASLTSTLLVAKGNANVSGFTALHAAAVPPRGGARPA
                     PRPAKEEAQLLSLRLDATQLRRLRLAAAHLDQTEQAFLVTALEHYIDHVVSPLLEGHC
                     GCLETEHKPRQGATANPFARQKSDLPQ"
     gene            7515..>8864
                     /locus_tag="VGP50_05030"
     CDS             7515..>8864
                     /locus_tag="VGP50_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026768.1"
                     /translation="MKPDDPPPGAPASPEAPPPASLTNVRLRGRGQAEPATKRGDLTQ
                     QLQAEVPAAPPPEPPRTPPKRGDLTQQLEAAATRKTAPPAVAPPPAPPPKAKAEPPPE
                     RKPAKAEMAAPQPKIEAPFERKPARAEAPAPQPKADPPVERKPAKAEAPAPQPKSDPP
                     AERRAVKAEPVPAPQAQVERPVEARIATPPKAESPRERNAATMKAEASKAPPPALEAP
                     PERRPAATKAEDASLGQDEVDALFDDVAPPPPPPPAVERKPVAAKSKPAAEPVEIEAE
                     TAAKPAADDPHASIPTGSTLREDDVRALLDPPGRRAAAAAPPAAETAAEAEAPEAEPE
                     DRAAAQPSPPVAESEEAAIAEGEPPTHETPDLHLIVDMPESAAGATPAADMEPAAAGS
                     TIDAIIDYWDGLRGEKPYPMLDTVDRSHVSASWPNCLLVAFTSMTSAEAAQPRMTRIG
                     "
BASE COUNT         1535 a         3064 c         2906 g         1359 t
ORIGIN      
        1 ttctgtctgt ccgtccttca aactccagcg tcgttgccgg catcggcgcc aggcatcgac
       61 ttaaacgtgg ctctcctcga cggaaacctg ctctacgccg tctccgtgaa gagctcgcgg
      121 ccaatgagca tgcggcggat ctccgaggtg ccggcgccga tctcgtaaag cttggcgtcg
      181 cgcaggagcc gtccggtcgc gtagtcgttg atatagccgt tgccgccgag cgcctggatc
      241 gcctcgagcg ccatgagggt ggctttctcc gacgcgtaga ggatcgcgcc ggcggcgtcc
      301 ttgcgtgtcg cctggccgcg gtcgcaagcg cgcgccaccg catagacata ggccttgcag
      361 gcattcatcg tcgtgtacat gtcggcgagc ttgccttgca tgagctggaa ctcgccgatc
      421 gcctggccga actgcttgcg ctcgtgcaca taaggaatca ccgtctcgag acaggactgc
      481 atgatgccga gcgggcccgc cgcgaggacc gctcgctcgt agtcgaggcc gctcatcagc
      541 actctgacgc catcattcac tttgccgagc acattctcct cgggcacttc gcagtcggtg
      601 aagaccagct ccgaggtcgg cgagccgcgc atgccgagct tgtcgagctt ctgcgccggg
      661 gcgaatcctt tcatgccctt ctcgatgagg aaagcggtga tgccgcgcgg cccggcattg
      721 gcgtcggtct tggcatagac caccagcgtc tcggcttcgg gtccgttggt gatccacatc
      781 ttgctgccgt tcagaacgta gcggccgcct ctcttgtcgg cacgcgtccg catgccgacg
      841 acatccgagc cggccgatgg ctcactcatg gcgagaccgc ccacatgctc gcccgacatc
      901 agcttcggca ggtatttgtg cttctgcgcc tcgctgccgt tgcgacggat ctggttgacg
      961 cagagattgg aatgcgcgcc ataggaaagg ccgacgctgg ccgagccgcg gcttacctcc
     1021 tccatggcga cgacatgggc gagatatccc atgccggtgc cgccatactc ctcctcgaca
     1081 gtgatgccat gaaggccgag ctcgcccatc ttcggccaga gctggcgcgg gaatttgtcg
     1141 ctgcggtcga tctccgcggc cagcggcgcg atctcgtcat cggcaaaggc gcgcaccgag
     1201 tcgcgcagct ggtcgatctc ggggccaagg tcgaagtcga gcatggcata gcggttggtg
     1261 ggcatcgggc gctccctgct caggcgagtc tacccccaca ttacatcctt gcaaggaaag
     1321 ggataatcgg gccgcagccc gccatagaac ggggacacat ggcattgaat ggccatcccc
     1381 cgattgcaag gagtatgtcg atgaattcgc gaaaagtcgt ccttccggcc ctggcgatcg
     1441 ccgtcctggt cggcggcgcg ggcctgggct cgctcacgac gcagcaggcc gccgctcagg
     1501 cgacgaccgc accgcagccg gccgagcagc atgagcggca tcatttctcg ccgacgcgcc
     1561 atgtcgaagg gcgcatcgcc tatctcaagg ccgagctcaa gatcaccgat gccgaggagc
     1621 agcaattcgc gcccgtggcc caggcgatgc gcgagaatgc caagaccatg gcgcaggcga
     1681 tggagcagcg ccacgggatg cgcgaccagc cgcagaccgc cgtgcagcga ctcgaggcaa
     1741 gggcgcaatt cgcaggcttg cgcgctcagg ccgaccaacg cttcgtcgat gccttcaagc
     1801 cgctctacgc gagtctttcc gccgatcaga agaaggcggc cgacgagttg ttgacaaggc
     1861 acggtcatcg gtagcatctg gttcgcggcc ggcgcggggg gcgcgcagcg cgccggctgc
     1921 gacatcctgt tcatgcgcgt gccaaaccaa cccgagactc aggaggacaa cgtgaacgtt
     1981 acccgtcgca ccgcgcttac caccgccgcc atgagcggcg tcgccctcgt cgccggcgtt
     2041 tcatcggccc aggcggagcc tcatcccaag atcaagcagg ccatccacgc cctcgagtcg
     2101 gcgagggacg acatgaagca tgccgcccat gatttcggcg gccaccgcga agacgccctc
     2161 cgcgcctgcg acgaggcgct ccgtcagctc catctcgcgc tggactgggc caatcatcac
     2221 taagacgcag gcttcgtttc gctttcctca gggccgcgtt gtcaccgaca actgcgcgtc
     2281 gtagacggcg atggcggtca tgttgatgag gccgcgcacg gtgatcgacg gcgcgacgat
     2341 atgcgccgcg ccgttgacac cgagcaagat cgggccgacc ggctggccgt cggccagcac
     2401 ccgcaaactg ttgaacgtga tattggccgc gtcgagattc ggcatgatca gcagattggc
     2461 cacgcctttg agcaaggcgt tggggaagat acgccggcga atctcctcgg agagggcggc
     2521 gtcggcatgc atctcgccct cgacctcgag cgccggcgcg cgctgacgca agagcgcgag
     2581 cgcctggcgc atcttgcgcg ccgacggcga atcggcgctg ccgaaatccg aatgcgacag
     2641 caacgccacc ttgggcggca cgccaaagcg acgcacggcg tcggcagcgc gtagcgtcat
     2701 ctcggccagc tgctccgccg agggatcgtc gaccacatgg gtgtcgcaga gaaagaaggt
     2761 gcccttgggc gtgatcagca cgctgagggc agcagcagcg ctcgcgccgg gcacgaggcc
     2821 cagaacatcg atgagatggc ggagatggcg gtgatattgg ccgatggcgc cacacagcat
     2881 cgcatccgcc tcgccacgcc gcaccatcag cgcggcgatg acggtggcac gtgtacgcac
     2941 gaggccgcgc gcgaagtcgg gcgacacgcc ctttcgctcc atgagcgagt gataaagcgt
     3001 ccaatactcg tgataccgcg ggtcatcctg gggatcgacg agcgtcacgg tcttgccgag
     3061 ggcgaggcgc agattgagct tctcgatgcg cctgagcacg acgtcgcggc ggccgatgat
     3121 gatcggcctg gcgaggccgt catcgaccac ctgctgcacg gcgcggagca cgcgctcgtc
     3181 ctcgccttcg gcatagacga ggcgcttggg ctcgccgcgc gcgcggtcga agacgggctt
     3241 catcaacagg ccggagcgga agacgaactg gctgaggcgt tggcgatagg cgtcgaggtc
     3301 gccgatcggc cgcgtcgcca cgcccgactc catcgccgcc ttcgccacgg ccggcgcgat
     3361 ctcgaggatg agccgcggat cgaacggctt cggaatgaga tagtcgggac cgaaggtgag
     3421 cgacgcgtcg ccataggccg cggctaccac gtccgaaggc tcggccagcg ccagatcggc
     3481 gagcgccgcg acacaagcga gcttcatcgc ctcgttgatc acggtggcgc cgacatcgag
     3541 ggcgccccgg aagatgaagg ggaagcacag gacgttgttg acctggtttg gatagtcggt
     3601 ccgcccggtg gcgatgatcg cgtcaggccg cacggccttg gccgcctcgg gacggatctc
     3661 gggatcggga ttggcgagcg ccaggatcag aggctgcgcc gccatcttcg ccaccatctc
     3721 gggcgtcaga acgtcgggcg ccgacaggcc gaggaagata tcggcgccgg cgatgacctc
     3781 gccgagcgtg cgcgcgttcg tctccttggc atagcggtcc ttgcgcggat ccatctcctc
     3841 tttgcggccg cgccacacca cgccgtattt gtccgtgacg atgatgttct cgagcggcag
     3901 ccccatatcg acgaggaggt cgaggcaggc gatcgccgcc gcgccggcgc ccgaagagac
     3961 gagccgcacc tccgcaagcg atttgccgat gagccgcaag ccgttgcgga tggcggcgcc
     4021 gacgatgatc gacgtcccgt gctggtcgtc atggaagacc gggatcttga ggcgctggcg
     4081 cagcttcgcc tcgatcgcga agcactccgg cgccttgatg tcctcgagat tgatgccgcc
     4141 gaaggtcggc tccagggccg cgatcacctc gacgagcttg tcgggatcgc gctcgtcgat
     4201 ctcgatgtcg aagacgtcga tgccggcgaa tttctggaag agcaccgcct tgccttccat
     4261 caccggcttg gcggcaagcg gcccgatcgc gcccaggccc agcactgccg tgccgttggt
     4321 gacgacgccg acgagattgg cgcgcacggt aagcgaagag gcctgctgcg ggtcggccgc
     4381 gatcgccagc gacgccgcgg cgacgcccgg cgaataggcg agcgccaggt cgcgctgggt
     4441 ggcgagcggc ttggtggcga cgatgccgat cttgccgggg gtgggcagcc ggtgatattc
     4501 gagcgcgtcg tcgcgcgagt cctcggccat gtgcgtcagg ctccctgagg cggtcgcgtt
     4561 cctctataca tcactcatgc gccgtcgtcc gccggccggc gcgcgcgatc cattcatgga
     4621 accggaggag gtggtgcggt cgagaagact cgaacttcca agccctttcg ggcactagca
     4681 cctcaagcta gcgcgtctac caattccgcc acgaccgcgc gggagcggcg gcgatctcgc
     4741 ctgccgctga cggggggcgt gattagcaaa tccccaggac catatcaagc aaaatgcccg
     4801 gaacatcgac agcaagcatc gaatggcgcg tgagcgaggc accggtcgcc tatgaggcgg
     4861 cggtcgcggc catggaggag cgcgtcgccg cgatccgcgc gggcagcgcg cccgaactgg
     4921 tgtggctcct caagcacccg ccgctctaca ctgcgggaac cagcgccaac gagcgcgacc
     4981 tcgtcgagcc cggtctcttt ccggtgcacc gcacgggccg cggcggccaa ttcacctatc
     5041 acggccccgg ccagcgcgtc ggctacgtga tgctcgacct ccagcgccgc ggccccgatc
     5101 tccgctgcta tgtgcgtgac ctcgaggagt ggctcatccg aacgctccgg tctttcggtg
     5161 tcgcgggcga gcgacggccc gggcgtgtcg gcatctggat cgccgagccg ggggggcgcg
     5221 agcgcaagat cgcggccatc ggcatccgcg tgcgccgctg ggtgagcttt cacggcgtct
     5281 ctctcaacgt cgatcccgac ctctcgcatt atcgcggcat cgtcgcttgc ggaatacagg
     5341 agcacggcgt cacctcgctc gccgaccaag gcgtccgcgc cagcatgcgc gaggtggacg
     5401 acgcaatgcg gcaggctttt gcgagcgtgt tcggcgccga gtagctcttc tcccgcgagc
     5461 gggagaggtg gcgcgcgcga agcgcgcgcc ggatgagggg acgcgtcaga agactcgcct
     5521 ttgcttgaag ctcacccggc gcggttagcg taagctatgt gctcaacgac tccgcgccta
     5581 cactcgcgtc ggcgcctggt agtcgacatt ggccgagctc cccgtggact cggtcgtgca
     5641 ggcgagccaa tggatcatgg tccacttgcc cgcctccgcg cgttgcttgg cgaggacgtg
     5701 cgcgatatgc cccaacgcca ccggcttttt tcccgaggtt ccggcgaggc tcatcaccat
     5761 ctcgccgctt tttcgtcgga acacgcccga ggccggagca atgcctggat aggcccagca
     5821 ggagtaagcc gaaagcgccg ttttgggctg ggcaacccac accgggtaca cgtcgacgtt
     5881 cagcaaaagc ttgtcgagga tcgtgtaccc tttgttgtcc ccgagcaccc gatccttcat
     5941 gcagtagaaa tggatctgga cgccgtccgg gacccaccac tgctctcctt tgaagcatcc
     6001 cccatgtccc gagacgatgt agttcttggt cattgtccaa cctccggcgg ccatcccgct
     6061 gcaagacggg agactactac ccgcggttgc gggcctgcgg ctgtgaaccg gatcacccgc
     6121 cgaccggcgt ttcgctgaga agaaatccgc tggtggcgca agcgcggtcc agcacgcttc
     6181 tcgaccccgc gccgcccgct ctcgccggca cgcagcagag gggattgatc gcggtggaaa
     6241 atattaacca atattgaaac gccgggaatg ttcgtcagta cttttttaag caacgtccat
     6301 gtaaattcag cgcgatagcg gtgccgcttg ctcggcggcg ttactacatt atatgggccc
     6361 ggcgtgcgga attcttcgca ccgttcgggg gtcttggaga tatagacgta tgcaagtcat
     6421 cgccgtcgcg tcacagaagg gcggttccgg caagaccacc ctcgcggggc atctcgcagt
     6481 ccaggcggat cgcaccggct gcgggcccgt ggcactggtc gataccgatc cgcagggcag
     6541 cttgtccgaa tggtggaacg cgcgcacggc agagacgccg ctcttcgcgc gcaccagcgc
     6601 cgggcgcctc gcccaggaca tcgagcgcat gcgcgagctc ggcatcaagc tgctcgtggt
     6661 cgacacgccg ccggccatcg aggcgacgat caccgaagtg atccgcctcg ccgacctcgt
     6721 cgtcatcccg gcgcggccga gccctcatga tctgcgcgcc gccggcgcca ctgtcgagtt
     6781 gatcgagcgg ctgggaaagc cgctgatctt cgtcgtcaac gccgcgacgc cccgcgcgcg
     6841 catcacttcg gaagcggtga tggcgctgtc ccagcacggc acgctggcgc cggtgatcct
     6901 gcatcaccgc accggtttcg ccgccgccat gatcgacggc cgcaccgtca tggaagtgcc
     6961 gggcgaagcg cgctccgcgg aggagatcga gctcctgtgg caatacctcg ccaaacgcct
     7021 caatcgcgat ttcgggacga tgctgccggc cgcgctgctc tcgtcggcgc ccaaggacgg
     7081 caatcccgag ctgggcgatc aggacggacc ctacgagcaa ggcgcatcat gagcagcagc
     7141 agcaccaaag tcgcatccct caccagcaca ttgctcgtcg ccaagggcaa cgccaatgtg
     7201 agtggcttca cggcgctcca cgccgcggcc gtcccgcccc ggggcggtgc ccgtccggcg
     7261 ccgcgcccgg caaaggaaga ggcacagctt ctctcgctcc gccttgacgc gacccagctc
     7321 cgccgtctgc ggctcgcggc cgcgcatctc gatcagacag agcaagcctt cctcgtcacg
     7381 gcgctcgagc attacatcga ccatgtcgtg tcgccgctcc tcgagggcca ttgcggctgt
     7441 ctcgagaccg agcacaagcc acgtcagggc gccaccgcca atccctttgc gcgacagaag
     7501 tcggaccttc cgcaatgaaa cctgacgatc cgccgccggg cgctccggcc agcccggagg
     7561 cgccaccgcc ggcatcgctg acgaatgtgc ggctgcgcgg ccgcggtcag gcagagcccg
     7621 cgacgaagcg cggcgacctc acccagcagc tccaggccga agtgccggcc gccccgcccc
     7681 cggaaccgcc gcgcaccccg ccaaagcgcg gcgatttgac gcagcagctc gaggcggccg
     7741 ccacacgcaa aaccgcaccg ccggcggttg ctccgccgcc ggcacccccg ccgaaagcga
     7801 aggccgagcc accgccggag cgcaagcccg cgaaagccga gatggccgcg ccgcagccga
     7861 agatcgaggc gccattcgag cgaaagcccg cgagagccga ggcgcctgca ccacagccga
     7921 aggccgaccc gccggtcgag cgcaaaccgg cgaaagccga ggcgcccgcg ccgcagccga
     7981 agtccgaccc gccggccgag cgcagagccg tgaaagccga gccggtgccc gcgccgcagg
     8041 cgcaggtcga gcggcccgtc gaagccagga tcgcgacccc gccgaaggcg gagtcgccgc
     8101 gggagcgcaa cgccgcgacg atgaaggccg aggcgtccaa ggcaccaccc cccgccctcg
     8161 aggcgccgcc cgagcgcagg cccgcggcaa ccaaagccga ggacgcttca ctgggccagg
     8221 acgaggtgga cgcgctcttc gacgacgtgg cgcccccgcc tccgcccccg ccggcggtcg
     8281 agcgcaagcc cgtggccgcc aagagcaagc cggccgccga gcctgttgaa atcgaggccg
     8341 agaccgccgc caaaccggcg gctgatgacc cgcatgccag catccccacc ggcagcacgc
     8401 tgcgcgagga cgacgttcgc gcgctgctcg atcctcctgg tcgccgcgcc gccgcagcag
     8461 caccgcctgc cgccgagacc gccgccgaag cggaagcgcc ggaagcagag cccgaggaca
     8521 gggcggccgc gcaaccgtcg ccgccggtcg cggagagcga agaggccgca attgccgaag
     8581 gcgagccgcc gacgcatgag acgcccgacc tgcaccttat cgtcgatatg cccgaatccg
     8641 cagccggggc cacacccgcc gccgacatgg agcctgccgc ggccgggagc accatcgacg
     8701 cgatcatcga ttactgggac ggcctgcgcg gcgagaagcc ctacccgatg ctcgacaccg
     8761 tcgatcgctc gcatgtcagc gcttcctggc ccaattgtct gctcgtcgcc ttcacgtcca
     8821 tgaccagcgc cgaagccgcg cagccgcgca tgacgcgcat cggc
//
LOCUS       DASYNS010000158         7943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3310652, whole genome shotgun sequence.
ACCESSION   DASYNS010000158 DASYNS010000000
VERSION     DASYNS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3310652"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..105
                     /locus_tag="VGP50_05035"
     CDS             <1..105
                     /locus_tag="VGP50_05035"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388228.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV8026769.1"
                     /translation="RRRSAEHDIEKIGEKLRAMMPWIAQNRLVDKSRN"
     gene            650..2218
                     /locus_tag="VGP50_05040"
     CDS             650..2218
                     /locus_tag="VGP50_05040"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV8026770.1"
                     /translation="MTRKTSTTGKAGDRVIIFDTTLRDGEQSPGASMNLDEKIKIALL
                     LEEMGVDVIEAGFPVASNGDFEAVREIAKLVRNSSVCGLSRAGRVDIDRAWEAVQYAK
                     HPRIHTFIATSKLHMKYKLQMEPDQVHQAVIDSVTHARNLCDDVEWSAEDATRSEHDF
                     LCRTVESAIKAGARTINIPDTVGYAVPHEFGALIGMLFERVPNIDKAVVSVHCHNDLG
                     LAVANSLAAVGAGARQVECTVNGLGERAGNASMEEIVMALRTRHDAIPFTSGIKTEYI
                     TRASRLVSGVTGFVVQPNKAIVGANAFAHESGIHQDGMLKHAGTYEIMTPESVGLTKS
                     SLVMGKHSGRHAFKSKLKELGFELGDNAVTDAFRRFKDLCDRKKDVYDEDITALVDDE
                     VVRGHDRVKFVSLQVIAGSKGPQSAELELEIDGTHHVAKTTGNGPVDATFNAIKEMVP
                     HTARLQLYQVHAVTEGTDAQAEVTVRLEENGKSVNGQGADPDTLVASARAYIHALNKL
                     LTKRAKTAPAAMTA"
     gene            2266..3306
                     /locus_tag="VGP50_05045"
     CDS             2266..3306
                     /locus_tag="VGP50_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEV8026771.1"
                     /translation="MFGNLLGMLSSDMAIDLGTANTLVYVKGRGIVLNEPSVVAIASV
                     KGRKQVLAVGDEAKMMLGRTPGNIQAIRPLRDGVIADFEVAEEMIKHFIRKVHNRRSF
                     ARPCIIVCVPSGSTAVERRAIQESAESAGASKVLLIEEPMAAAIGAGLPVTEPTGSMV
                     VDIGGGTTEVAVLSLGGIVYSRSVRVGGDKMDEAIIAYIRRNHNLLVGESSAERIKKE
                     IGSACMPEDGEGRVMEIKGRDLMNGVPKEIVISERQIAESLAEPVGAIVEAVKVALEH
                     TAPELAADIVDKGIVLTGGGALLGNLDYVLRHATGLPVSIADDPLSCVALGTGRALEE
                     MKTLKNVLISMY"
     gene            3471..4289
                     /gene="mreC"
                     /locus_tag="VGP50_05050"
     CDS             3471..4289
                     /gene="mreC"
                     /locus_tag="VGP50_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV8026772.1"
                     /translation="MFIVLGKADVLLFDRLRILIVDGSAPILEAVSQPIATAASGVHN
                     IGAMFDVYGENQRLRDENARLLEWQEVARGLAAENTRLRGLVKLAPEGARSDVSAQVI
                     ADSGGAFARNVLVSAGRRDGVARGQAAVTGEGLVGRVAEVGDRASRILLLTDLNSRIP
                     VVLESSRERAVMAGDNSVQPRLLYLPATAVVKAGERIVTGGAGGVFPPGLPVGVVASV
                     DGPNVRVEPYAELPRLDYVRIIDFGLEGLLPPNAVPVAKPARGPKVVGDPDPGR"
     gene            4298..4816
                     /gene="mreD"
                     /locus_tag="VGP50_05055"
     CDS             4298..4816
                     /gene="mreD"
                     /locus_tag="VGP50_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV8026773.1"
                     /translation="MRFDGFQRDQSLPTRLVPFTTTFLFTLISVVPLHIPGFAVVTPS
                     FALMAIYHWTIYRSDLLPPVAVFGTGMLLDLLNGAPYVGTSSLLLLLLRQLLLSQRRF
                     FVNRPFAYLWAGFLLVTTAMTVLEWALVSLLHGSVLDAEAPIFGAALTAASFPVGSYL
                     LGRTQRGLLMRG"
     gene            4828..6738
                     /gene="mrdA"
                     /locus_tag="VGP50_05060"
     CDS             4828..6738
                     /gene="mrdA"
                     /locus_tag="VGP50_05060"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEV8026774.1"
                     /translation="MDRENNTKNQLLTRRALLLGGAQGALFATLAGRMYYLQVVQADR
                     YAMLADENRINIRLVAPPRGRILDRFGAVLADNRPTYRVVLVAEQAGDIPSTVNAVGT
                     LIPVSDTDRRRVLRDLHNKHSFVPVMIRENLSWEEMARIEVNTLELPGVSIEQGRLRH
                     YPFGDNTAHVLGYVAAVSEEELTGEDPLLELPDFRIGKSGIEKSHDLDLRGSAGTSQV
                     EVNAYGRVVRELAREEGIAGQDIALTIDMALQDFAGRRCAVEKSAACVLMDAWTGDVL
                     SMVSSPGFDPHGFVSGWSQAEWDTLQNDPAKPLNNKAISGTYAPGSTFKPVVAMAALE
                     TGVITPETRFNCPGFFQFGNVTFHCWKKGGHGSMDLHDGIKHSCDVYFFELTRRLGID
                     HIADMARRFGLGSAVGIDIPGEATGLIPSSNYMFHGSNGLYKWQPGDTISAGIGQSVI
                     AVTPLQLATYAARLVTGRAVVPHLTRDGGVMAADSDAPPSREFPAIKLARKDLALVVS
                     GMNAVVNEQGGTAYAARIAEPGMQMGGKSGTSQVRHITQAEREHGLRKIKDVPWKERD
                     NALFISFAPVSAPRYVCAVIVEHGGESAGGGSAVAAPICRDVLRETQKRDPARRVPER
                     PFGSETTVAQGS"
     gene            6748..7896
                     /gene="rodA"
                     /locus_tag="VGP50_05065"
     CDS             6748..7896
                     /gene="rodA"
                     /locus_tag="VGP50_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEV8026775.1"
                     /translation="MYQLLDGARPAPNLTERIRSLNWTLVLLLTVIAGIGFAMLYSAA
                     NGSWQPWAGKQAIRFGVAVAIMLTVALVDLRFWLRWAHWMHGAAFLLLIAVAVVGHFG
                     MGAQRWIDLGVIQLQPSEIMKVTTVLSLARYFHGMTPEDMGRPFRLILPALIVLVPAA
                     IVLKQPDLGTAMMMVLTGGAMFLIAGVRGRLFVLVLGAGAAVVPVAWRFLRDYQKNRI
                     YTFLDPENDPLGAGYHSLQSKIAIGSGGVFGKGFMQGSQAHLSFLPEKHTDFIFTMLA
                     EEWGLMGGLVLLLLYSLVLVYGYAIAFRSRSPFGRLLALGITANFFLYVFINTAMVMG
                     LIPVVGVPLPLISYGGTSMLTVMFGFGLLMGVYIHRDTRIGRFGEAEF"
BASE COUNT         1312 a         2682 c         2581 g         1368 t
ORIGIN      
        1 cgccgccgct cggccgagca cgacatcgag aagatcggcg agaagctccg cgccatgatg
       61 ccgtggatcg cccagaaccg gctggtcgac aagtcgcgga attaagggcg agatcgaact
      121 ggcgtcaccc cccccccacc ctgaccctcc ccctcaaggg ggaggggaaa tgactcgtgc
      181 cgattgactt tcccctcctc ccttgagggg gagggagagg gtgggggtag ccgcgcgacg
      241 gatattccta agcgtcgcgc acgcgctcga atggttaact atatcttgcg ggcgggcgcg
      301 gaaacgggat agagatatcc acgcctccgg cgtttaactt taagggcttt ttaaggctgc
      361 gggggcaatt ttaaagggcc ggcgcggacg ccggcaaaga gcaggcgaga tgatcggcac
      421 gggagcttgc ggcggaggag gccaggcgct tttgcgccgg ccggcttacg tcccgatgct
      481 tctccgggta tgtggtcttg ccaagctctc gtggagccag gggtcgctgc gacttatcgg
      541 cccctgagcg catgtcgcgc gccgaaggcg tgtgctgcgt tcagggggcg tctgtcgagg
      601 cactttccca ttgctcccac ttaagaggat cagcgccatg agcgccacca tgacccgaaa
      661 gacttccacg accggcaaag ccggcgaccg cgtcatcatt ttcgacacga cgctgcgcga
      721 cggcgagcag tcgcccggcg cgtcgatgaa tctcgacgag aagatcaaga tcgcgctgct
      781 gctcgaggag atgggcgtcg acgtgatcga ggccggcttc cccgtcgcgt ccaacggcga
      841 tttcgaggcg gtgcgcgaga tcgccaagct ggtcaggaat tcgagcgtct gcggcctgtc
      901 gcgcgccggg cgagtcgaca tcgaccgcgc ctgggaagcg gtgcagtatg cgaagcatcc
      961 gcgcatccac accttcatcg ccacctcgaa gctgcacatg aaatacaagc tccagatgga
     1021 gcccgatcag gtgcaccagg cggtgatcga cagcgtcacc catgcccgca acctctgcga
     1081 tgacgtcgaa tggtcggccg aggacgcgac gcgcagcgag cacgacttcc tctgccgcac
     1141 cgtcgagagt gcgatcaagg ccggggcgcg caccatcaac atccccgata cggtcggcta
     1201 tgccgtgccg cacgagttcg gggcgctcat cggcatgctc ttcgagcgcg tacccaatat
     1261 cgacaaggcg gtggtctcgg tccattgcca caacgacctg ggcctcgccg tcgccaactc
     1321 cctggccgcg gttggcgcgg gggcgcggca ggtcgaatgc acggtcaacg gcttgggcga
     1381 gcgcgccggc aatgcctcga tggaggagat cgtcatggcg ctgcgcacgc gccacgacgc
     1441 catccccttc acctcgggga tcaagacgga atacatcacc cgcgcgtcgc gcctcgtctc
     1501 gggcgtgacc ggcttcgtcg ttcagcccaa caaggcgatc gtcggcgcca acgccttcgc
     1561 ccatgagagc ggcatccacc aggacggcat gctgaagcat gccggcacct acgagatcat
     1621 gacgccggaa tcggtcggcc tcacgaagtc gagcctggtg atgggcaagc attcgggccg
     1681 ccatgccttc aagtccaagc tcaaggagtt gggcttcgag ctcggcgaca atgccgtgac
     1741 cgacgccttt cgccgcttca aggatctctg cgaccgcaag aaggacgtct acgacgaaga
     1801 catcacggcg ctcgtcgacg acgaggtcgt ccgcggtcat gaccgtgtca aattcgtgtc
     1861 gctccaggtg atcgccggct ccaagggccc gcaaagcgcc gagctcgagc tcgagatcga
     1921 cggcactcac cacgtggcga agaccaccgg caacggcccg gtcgacgcca ccttcaacgc
     1981 catcaaagag atggtgccgc acaccgcgcg cctgcagctc taccaggtgc atgcggtgac
     2041 cgagggcacc gacgctcaag ccgaagtcac ggtccgcctc gaggagaacg gcaagtcggt
     2101 caacggccag ggcgccgatc ccgacacgct ggtcgcctcg gcccgggcct atatacacgc
     2161 cttgaacaag cttttgacca agagagcaaa aacggcgcct gccgcgatga cggcgtgagg
     2221 aaaaagcact tctcgcaatc ctaaccattg ggtcaacaga cgcacatgtt tggaaatctc
     2281 ttgggcatgc tctcctccga tatggcgatc gacctcggaa cggcgaacac cctcgtctac
     2341 gtcaaaggcc gcggcatcgt tctgaacgag ccctcggtcg tcgcgatcgc cagcgtcaag
     2401 ggccgcaagc aggtcctcgc ggtgggcgat gaagccaaga tgatgctggg ccgaacgccc
     2461 ggcaacattc aggccattcg cccgctgcgc gacggtgtca tcgccgattt cgaagtcgcg
     2521 gaggagatga tcaagcactt catccgcaag gtgcacaacc gccgcagctt cgcccggccc
     2581 tgcatcatcg tctgcgtgcc ctcgggctcg acggcggtcg agcgccgcgc catccaggaa
     2641 tcggcggaga gcgccggcgc cagcaaggtc ctcctcatcg aggagccgat ggcggcggcg
     2701 atcggcgccg ggctgccggt gacggagccg acgggatcga tggtcgtcga catcggtggc
     2761 ggcaccaccg aagtcgcggt cctctcgctc ggcggcatcg tctactcgcg ctcggtgcgc
     2821 gtcggcggcg acaagatgga cgaggcgatc atcgcctata tccgtcgcaa ccataacctg
     2881 ctggtcggcg aatccagcgc cgagcgcatc aagaaggaaa tcggctccgc ctgcatgccc
     2941 gaagatggcg agggccgggt catggagatc aagggccgcg acctcatgaa tggcgtgccc
     3001 aaggagatcg tcatcagcga gcggcagatc gccgaaagct tggccgagcc cgtgggcgcc
     3061 atcgtcgagg cggtgaaggt cgcactcgag cataccgcac ccgagctcgc cgcggacatc
     3121 gtcgacaagg gtatcgtgct caccggcggc ggcgctctcc tcggcaatct cgattatgtg
     3181 ctgcgccatg ccacgggcct gccggtatcg atcgccgacg acccgctgtc ctgcgtcgca
     3241 ctcggcaccg ggcgtgccct cgaagaaatg aaaacgttaa agaatgtgtt aatcagcatg
     3301 tactaggctg cgcttccagg taaatctacg gtcgtgcccc ggttatccgg cgccgccagg
     3361 ctggagtagt ccttttgcgg gagcggtcgg gagcgattga caggttcgcg gcgccattca
     3421 gggcgatcac gcagcgtgct gccgtaccgt tcttcatctt cctgtctgcc atgttcatcg
     3481 tgttgggcaa ggcggatgtc ctcctcttcg accggctgcg catcctcatc gtcgacggct
     3541 cggcgccgat cctcgaggcc gtctcccagc cgatcgccac ggccgcgtcg ggggtgcaca
     3601 acatcggcgc gatgttcgac gtctatggcg agaaccagcg cctgcgcgac gagaatgcgc
     3661 gtctcctcga atggcaggaa gtcgcgcgcg gccttgccgc cgagaatacc cgcctgcgcg
     3721 gcctcgtgaa gctcgcgccc gaaggcgcgc gtagcgacgt ctccgcgcaa gtcatcgccg
     3781 attcgggtgg cgcctttgcc cgcaacgtcc tggtaagtgc cggccggcgc gatggcgtcg
     3841 cgcgcggcca ggccgcggtc accggcgaag ggctggtcgg ccgcgtcgcc gaggtcggcg
     3901 accgcgcctc ccgcatcctg ctgctcaccg acctcaactc gcgcatcccc gtcgtgctcg
     3961 aatcctcgcg tgagcgcgcc gtcatggcgg gcgacaactc cgtccagccg cgtctccttt
     4021 acctgccggc caccgccgtc gtgaaggcgg gcgagcgcat cgtcaccggc ggcgccggcg
     4081 gcgtcttccc gccggggctg cccgtgggcg tggttgcgag cgtcgacggc ccgaacgtcc
     4141 gcgtcgagcc atatgccgag ctgccgcgcc tcgattatgt gcgcatcatc gatttcggcc
     4201 ttgagggctt gctgccgccc aacgccgtgc cggtggcgaa gccggccagg ggacccaagg
     4261 tcgtcggcga ccccgacccg gggcgatgag cgccttcatg cgcttcgacg gcttccagcg
     4321 cgatcagagc ctgcccaccc ggctcgttcc cttcaccacg acctttctct tcacgctcat
     4381 ttccgtggtg ccgctgcata tcccgggttt cgcggtggtg acgccctcct tcgcgctgat
     4441 ggcgatctat cactggacca tctaccgctc cgacctgctg ccgcccgtcg ccgtgttcgg
     4501 caccggcatg ctgctcgatc tgctgaacgg cgcgccttac gtcggcacgt cgtcgctcct
     4561 gctgctgctg ctgcgccagc tgctgctgag ccagcgccgc ttcttcgtca atcggccctt
     4621 cgcctatctc tgggcgggct tcctcctggt gacgacggcg atgacggtcc tcgaatgggc
     4681 gctggtgagc ctgctgcacg gttcggtgct cgacgccgag gcgccgatct ttggtgcggc
     4741 gctgaccgcc gcgagctttc ccgtcggcag ctacctgctc ggccgcaccc agcgcggcct
     4801 gctgatgcgc ggctaggcgg gcgagcgatg gaccgcgaga acaacaccaa gaaccagcta
     4861 ctgacgcgcc gggcgctgct gctgggcggt gcccaggggg cgctgttcgc gacgctcgcc
     4921 ggtcgcatgt attacctgca ggtcgtgcag gccgaccgct atgcgatgct cgccgacgag
     4981 aaccgcatca acatccgcct ggtggcgccg ccgcgcgggc gcatcctcga tcgcttcggc
     5041 gcggtgctcg ccgacaaccg cccgacctat cgcgtcgtcc tggtggccga gcaggccggc
     5101 gacatcccgt cgacggtcaa cgccgtcggc acgcttattc ccgtctccga caccgaccgg
     5161 cgccgcgtgc tgcgcgatct ccacaacaag cacagcttcg ttccggtgat gatccgtgag
     5221 aatctgagct gggaggagat ggcgcgcatc gaggtgaaca cgctcgagct gccgggcgtg
     5281 tcgatcgagc agggccggct ccgccactac cccttcggcg acaacaccgc ccatgtgctg
     5341 ggctatgtcg cggccgtgtc ggaggaggag ttgaccggcg aggacccgct gctggagctg
     5401 cccgacttcc gcatcggcaa gagcggcatc gaaaagtcgc atgacctcga cttgcgcggc
     5461 agcgcgggca cgagccaggt cgaggtcaat gcctatggcc gcgtcgtgcg cgagctggcg
     5521 cgcgaggaag gcatcgccgg ccaggacatc gccctcacca tcgacatggc gctccaggat
     5581 ttcgccgggc ggcgctgcgc cgtcgagaag agcgcggctt gcgtgctcat ggatgcgtgg
     5641 accggcgacg tgctctccat ggtgtcgagc ccgggcttcg acccgcacgg tttcgtgtcg
     5701 ggctggagcc aggcggagtg ggacaccctg cagaacgatc cggcgaagcc gctcaacaac
     5761 aaggcaatca gcggcaccta tgcgccgggc tcgaccttca agccggtggt ggcgatggcc
     5821 gcactcgaaa ccggcgtcat taccccggag acgcgcttca actgtccggg cttcttccag
     5881 ttcggcaatg tgacgttcca ctgctggaag aagggcggcc atggcagcat ggatctgcat
     5941 gacggcatca agcattcctg cgacgtctat ttcttcgagc tgacgcgccg tctcgggatc
     6001 gatcacatcg ccgacatggc caggcgcttc ggcctcggca gcgctgtcgg catcgacatc
     6061 cccggcgagg cgaccggcct catcccgagc agcaactaca tgttccatgg ttcgaacggg
     6121 ctctacaaat ggcagcccgg cgacaccatc agcgccggca tcggccagag cgtcatcgcg
     6181 gtcacgccac tgcagctcgc cacctatgcc gcccgcctcg tcaccggccg cgccgtcgtc
     6241 ccgcatctga cccgcgacgg tggcgtcatg gcggccgaca gcgacgcgcc gccctcgcgc
     6301 gagttccccg ccatcaagct cgcgcgcaag gatctggcgc tggtggtgag cggcatgaat
     6361 gcggtggtga acgagcaggg cggcacggcc tatgcggcgc gcatcgccga gcccggcatg
     6421 cagatgggcg gcaagtcggg tacgtcgcag gtgcgccaca tcacgcaagc cgaacgcgag
     6481 catgggctgc gcaagatcaa ggacgtgccg tggaaggagc gcgacaatgc gctgttcatc
     6541 tccttcgccc cggtgagcgc gccgcgttac gtctgcgccg tgatcgtcga gcatggcggc
     6601 gagagcgccg gcggcggctc cgccgttgcc gcgccgatct gtcgcgacgt gctgcgcgaa
     6661 acgcagaagc gcgatccggc gcggcgcgtg ccggagcgcc ccttcggcag cgaaaccacg
     6721 gtcgcgcagg gatcataggt caacgcaatg tatcagctcc tcgacggcgc gcggccggcg
     6781 cccaacctca ccgagcggat ccgcagcctc aactggacgc tcgtgctgtt gctgacggtg
     6841 atcgccggca tcggctttgc gatgctctat tcggcggcca atggtagttg gcagccctgg
     6901 gccggcaagc aggcgatccg cttcggcgtg gccgtcgcga tcatgctgac ggtcgcgctg
     6961 gtcgatctgc gcttctggct cagatgggct cattggatgc atggcgccgc gtttctgctc
     7021 ctgatcgccg tcgccgtggt cgggcatttc ggcatgggcg cccagcgctg gatcgatctc
     7081 ggcgtgatcc agctccagcc ctccgagatc atgaaggtca ccaccgtcct gtcgcttgcc
     7141 cgctatttcc acggcatgac gcccgaggac atgggacggc cgttccggct gatcctcccc
     7201 gccctcatcg tgctggtgcc ggcggcgatc gtgctgaagc agcccgatct cggcacggcg
     7261 atgatgatgg tgctgaccgg cggcgccatg ttcctgatcg ccggcgtgcg cggccggctc
     7321 ttcgtgctgg tcctcggcgc cggggctgcg gtggtgccgg tcgcctggcg cttcctcagg
     7381 gattaccaga agaaccgcat ctacaccttc ctcgatcccg agaacgatcc gctgggcgcg
     7441 ggctatcaca gcctgcagtc gaagattgcc atcggctcgg gcggcgtctt cggcaagggc
     7501 ttcatgcagg ggagccaggc ccatctttcc ttcctgccgg agaagcacac cgacttcatc
     7561 ttcaccatgc tggccgagga atggggcctg atgggtggcc tggtgctgtt gctgctctat
     7621 tcgctcgtgc tggtctacgg ctacgccatc gccttccgca gccgcagccc cttcggccgc
     7681 ctgctggcgc tcggcatcac cgccaatttc ttcctctatg tattcatcaa cacggcgatg
     7741 gtgatgggcc tgatcccggt ggtgggcgtg cccctgccgc tcatctccta tggcggcacc
     7801 tcgatgttga cggtgatgtt cggcttcggc ctgttgatgg gcgtctacat ccaccgcgac
     7861 acccgcatcg gccgcttcgg cgaagccgag ttctgagccg ccggcctcga caagaccggg
     7921 cccggttgat atatacgcgc ctc
//
LOCUS       DASYNS010000159         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3000223, whole genome shotgun sequence.
ACCESSION   DASYNS010000159 DASYNS010000000
VERSION     DASYNS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3000223"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VGP50_05070"
     CDS             complement(<1..1102)
                     /locus_tag="VGP50_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8026776.1"
                     /translation="MTDTALRHGRTSTGPLLPDLLPLTGRALDAADAFLAAAKGNLAA
                     AIAPGGRVDPALLEGRQFAAHGYAWMATYVEALRQMRLWAERLELVGGLREQEALMLQ
                     IAYGEYLAQLAGGIALSQVEVMRPHDLGLGDEEIAAFRRPDVVQLITHGNSEAARARL
                     AELLRDALDTGDFGHLALGEEALDMVRDQFRRFGAEEVAPFAHAWHLEDKLIPLEVVE
                     AMSALGVFGLTVPEEFGGLGLGKLSMCIVSEELSRAYIGVGSLGTRSEIAAELIRLGG
                     TAQQKAHYLPKLASGEILPTAVFTEPNTGSDLGSLRTRAVREGDVYRIYGAKTWITHA
                     ARADLMTLLVRTDPATSDYRGLSMFLAAKPRGS"
     gene            complement(1263..2558)
                     /gene="ccrA"
                     /locus_tag="VGP50_05075"
     CDS             complement(1263..2558)
                     /gene="ccrA"
                     /locus_tag="VGP50_05075"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEV8026777.1"
                     /translation="MSEVAQVIPFTPGAAAGRPVKDLYEVGEIPPLGHVPKRMYGWAI
                     RRDRHGPPDTAMQVEVLPTPELAPDEVLVLVMAAGVNYNGVWAALGKPISPFDVHKAE
                     FHIAGSDASGVVWATGSKVKRWKVGDEVVVHCNQDDGDDEECNGGDPMFSPSQRIWGY
                     ETPDGSFAQFARVQGRQLMERPRHLTWEESGCYVLTLATAYRMLFGHRPHVLRPGHNV
                     LVWGAAGGLGSMAIQLIATAGANAIAVISEDDKRDFVMQLGARGVINRKKFDCWGRLP
                     DVNDSGGYGEYMKKCREFGKAIWQYTGKGNDVDFVFEHPGEQTFPVSCFIVKRGGMVV
                     FCAGTTGYNLTFDARFVWMRQKRIQGSHFANLLQASQANRLVVERRIDPCMSEVFSWE
                     DIPRAHMKMWRNEHKPGNMAVLVSAKRPGMRTIEEAAQA"
     gene            complement(2706..>3554)
                     /locus_tag="VGP50_05080"
     CDS             complement(2706..>3554)
                     /locus_tag="VGP50_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV8026778.1"
                     /translation="AARWAPPPSKFVDIAGMSVHLRDEGPADDPLPILLIHGTSASLH
                     TWQGWARALRDHHRVVSFDLPGFGLTGPSPDGDYTMAANVRFVRALMDRLGIARCVLA
                     GNSLGGNIAWETAYAMPDRVAKLVLVDSGGYPLSSTAVPIGFRIARIPVLNRLTGSLL
                     PRDLIEASLRSVYGDPAKVTPELVDLYFAMTIREGNRQALVQRFEQSDFGGQASRIAT
                     LKLPTLILWGGRDRLIPPDNAEHFHRDIAGSRLVVFDDLGHVPQEEDPERSAAALDDF
                     LGYSEK"
BASE COUNT          598 a         1187 c         1204 g          565 t
ORIGIN      
        1 cgctgccacg cggcttcgcc gccaggaaca tgctgaggcc gcggtagtcg ctggtcgcgg
       61 gatccgtgcg caccagcaaa gtcatgaggt cggcacgcgc cgcatgggtg atccaggtct
      121 tggcgccata gatgcgatag acgtcgccct cgcgcaccgc ccgcgtcctg aggctgccga
      181 ggtcggagcc ggtgttgggt tcggtgaaga ccgccgtcgg caggatctcg cccgaggcga
      241 gcttcggcag ataatgcgcc ttctgctgcg ccgtgccgcc caggcggatg agctcggccg
      301 cgatctcgga gcgcgtgccg agggagccga cgccgatata ggcccgcgac agctcttcgg
      361 agacgatgca catcgagagc ttgccgaggc cgaggccgcc gaactcctcc ggcaccgtga
      421 ggccgaagac gccgagcgcc gacatcgcct cgacgacctc gagcgggatc agcttgtcct
      481 cgagatgcca ggcatgggcg aagggagcga cctcttcggc gccgaagcgg cggaattggt
      541 cgcgcaccat gtcgagggcc tcttcgccca gcgccagatg gccgaaatcg ccggtgtcga
      601 gggcatcgcg cagcagctcg gcaagccgcg cccgcgccgc ctcgctgttg ccgtgggtga
      661 tgagctgcac cacgtcggga cggcggaagg ccgcgatctc ctcgtcgccg aggccgagat
      721 catggggccg catcacctcg acctgggaga gcgcgatacc gcccgcgagc tgcgccagat
      781 attcgccgta ggcgatctgc agcatcagcg cttcctgctc gcgcaggccg ccgacgagct
      841 cgaggcgctc ggcccagagg cgcatctgcc tgagcgcctc gacataggtc gccatccagg
      901 catagccatg ggcggcgaat tggcgcccct ccagcagcgc cggatcgacc cggccgcccg
      961 gcgcgatcgc ggcggcgagg ttgcccttgg ccgccgccag gaacgcatcc gccgcatcga
     1021 gcgcccggcc ggtgagcggg aggagatcgg ggagcagcgg accggtcgag gttctaccgt
     1081 gacgcaaagc ggtatcagtc atcgacgctc ctcatgcccc tctccccttg tgggagaggg
     1141 aggggcccgt cgcgtcagcg acgggagggt gaggggaaag agcgcgcctg ccgcacccct
     1201 caccctgtcc ctctccccca aggggagagg ggacgctcgg cgcggcctat gcccgtccag
     1261 ccctacgcct gtgccgcttc ctcgatggtg cgcatgccgg ggcgcttcgc cgacaccagg
     1321 acggccatgt tgccgggctt gtgctcgttg cgccacatct tcatatgcgc gcgcgggatg
     1381 tcttcccacg agaacacttc ggacatgcag ggatcgatgc gccgctcgac gacgagccgg
     1441 ttcgcctggc ttgcctgaag cagattggcg aagtggctgc cctggatgcg cttctgccgc
     1501 atccacacga agcgcgcgtc gaaggtgagg ttgtagccgg tcgtcccggc gcagaacacc
     1561 accatgccgc cgcgcttcac gatgaagcag gagaccggga aggtctgctc gcccgggtgc
     1621 tcgaagacga aatcgacgtc gttgcctttg ccggtgtact gccagatcgc cttgccgaac
     1681 tcgcggcact ttttcatgta ttcgccatag ccgcccgagt cgttgacgtc gggaagccgg
     1741 ccccagcaat cgaatttctt gcggttgatc acgccgcgcg cgcccagctg catgacgaag
     1801 tcgcgcttgt cgtcctcgga gatgacggcg atggcgttgg cgccggcggt ggcgatgagc
     1861 tggatcgcca tcgagcccaa gccgcccgcc gcgccccaga ccagcacgtt gtggcccggc
     1921 cgcagcacat gcgggcgatg gccgaacagc atgcggtagg cggtggcaag cgtcagcacg
     1981 tagcagccgc tctcttccca ggtgagatgc cgcggccgct ccatgagctg gcgcccttgc
     2041 acgcgggcaa actgggcgaa ggagccgtcg ggcgtctcat agccccagat gcgctggctc
     2101 ggcgagaaca tgggatcacc gccattgcat tcctcgtcgt cgccgtcgtc ctgattgcaa
     2161 tggacgacga cctcgtcgcc gaccttccag cgcttgacct tggagccggt cgcccagacc
     2221 acgcccgacg cgtcggagcc ggcgatatgg aattcggcct tgtgcacgtc gaagggcgag
     2281 atcggcttgc cgagagcggc ccagacgccg ttgtagttga cgcccgccgc catcaccagg
     2341 accagcacct catccggcgc cagctccggg gtcggcagca cctccacctg catcgccgtg
     2401 tccggcggcc cgtgacggtc gcggcggatc gcccagccat acatccgctt cggcacatgt
     2461 cccaaaggcg ggatctcgcc gacctcgtag aggtccttga ccggacggcc tgccgccgct
     2521 ccgggagtga acgggatcac ctgagcaacc tcgctcatcg cttgcactcc attgatgccg
     2581 ttgccgcgcc gctttcgttg cggcgcaaca gagataagaa gataaacact tgtgtaacgg
     2641 ggacgttgcc gtattgcaat gcacaaaagg cgttttgtgt cggcatttcc tgttgacaag
     2701 aaatattatt tctcagaata ccccaagaag tcgtcgagcg ctgccgcact tcgctccgga
     2761 tcctcttcct gcggcacatg gcccaggtcg tcgaagacga cgagccggct gccggcgata
     2821 tcgcgatgga aatgctcggc gttgtcgggc gggatcaggc ggtcgcggcc gccccagagg
     2881 atgagcgtcg gcagcttgag cgtggcgatc cggctggctt ggccgccgaa gtcggattgc
     2941 tcgaagcgtt ggaccagcgc ctgccggttg ccctcgcgga tggtcatggc gaaatagaga
     3001 tcgacgagtt ccggtgtgac cttggccgga tcaccataga cgctgcgcaa gctcgcctcg
     3061 atgaggtcgc gcggcagcag ggaccccgtc aaccggttga gcaccgggat ccgcgcgatg
     3121 cggaacccga tgggcaccgc ggtcgaggac agcggatagc cgcccgaatc gaccagcacc
     3181 agcttcgcga cgcggtccgg catggcatag gccgtctccc aggcgatgtt gccgcccagc
     3241 gaattgccgg cgagcacgca acgcgcaatg ccgagccgat ccatcagcgc ccgcacgaag
     3301 cggacattgg ccgccatggt gtaatcgccg tcgggcgacg ggccggtcag gccaaatccc
     3361 ggaagatcga aggagacgac gcggtggtgg tcgcgcaacg cccgcgccca gccctgccag
     3421 gtgtgcaggc tcgccgaggt gccgtggatc agcaggatgg ggagaggatc gtcggccggg
     3481 ccctcgtcgc gcagatggac gctcatcccg gcgatatcga cgaatttgga gggcggcggc
     3541 gcccagcgcg cggc
//
LOCUS       DASYNS010000160         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4872797, whole genome shotgun sequence.
ACCESSION   DASYNS010000160 DASYNS010000000
VERSION     DASYNS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4872797"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VGP50_05085"
     CDS             <1..284
                     /locus_tag="VGP50_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEV8026779.1"
                     /translation="ALTGDGIPQIWERVEHQAATLLASGERERRRAEQARAALWSEIG
                     EGLVDLFKADQQVAAKLGDIEAQVADGTLTPSAAARAALAAFRSRARTT"
     gene            371..676
                     /gene="rpmB"
                     /locus_tag="VGP50_05090"
     CDS             371..676
                     /gene="rpmB"
                     /locus_tag="VGP50_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV8026780.1"
                     /translation="MARRCIITGKGVQVGNNVSHANNKTKRRFLPNLQVTSLLSDALG
                     APIRLRLSTHGIRTVEHNGGIDAYLLGTSDSKLSTEARRLKRRVVAAAARKAEATQS"
     gene            1002..1187
                     /locus_tag="VGP50_05095"
     CDS             1002..1187
                     /locus_tag="VGP50_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026781.1"
                     /translation="MDEESIGFFSVLLIVTAIALTHPFQLNRVVLTLPDIRWQDMVEA
                     SACGPDVAAGCTQTPTP"
     gene            complement(1213..>1999)
                     /locus_tag="VGP50_05100"
     CDS             complement(1213..>1999)
                     /locus_tag="VGP50_05100"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HEV8026782.1"
                     /translation="GLRRLVAYQDLRYAQLYLERLAPIRDADIRTAAGGRLLREVARQ
                     LAVRMSFEDVIRVAQAKTDPARLRRIRAELGVGEDDPMAIVDFFKPGIVELCSILPSL
                     LARPILGLAERRGWLDRAYWGMEVRSTSVSGYLRLRLLAKLRRWRRRTHRFAEEQVAI
                     ERWLQLILDAVTLSPELARAVAECARLIKGYGDTQARGVANYRQIESRIIRPALAGGY
                     PAAAAIDAVASALSAALSDPDGARLEQCLAEIERRAGRGVAAE"
BASE COUNT          351 a          681 c          658 g          309 t
ORIGIN      
        1 cggccctgac gggcgacggc atccctcaga tatgggagcg cgtggaacat caggccgcga
       61 cgctgctcgc cagcggtgag cgcgagcgcc gacgcgccga gcaggcgcgc gccgcgctct
      121 ggagcgagat cggggagggg ctcgtcgatc ttttcaaggc cgatcagcag gtcgccgcca
      181 agcttggcga catcgaggcg caggtggccg acggcaccct taccccctcg gctgcagccc
      241 gggcggcgct cgccgccttt cgcagccggg cgcgcacgac ttgacgggga tgcggctggt
      301 ggcttagaaa ccgtcccttg gcgcggtctt ccggcgagga gggccgtgtt ctgctttccg
      361 aggatcggtc atggcgagac gatgcatcat caccggcaag ggcgttcagg tcggcaacaa
      421 tgtgagccac gccaacaata agaccaagcg ccgctttttg ccgaaccttc aggtcacctc
      481 gctcttgtcc gacgcgctcg gtgctccgat ccgcctgcgt ctctcgaccc acgggatccg
      541 caccgtcgag cacaatggcg ggatcgacgc ttatctgctg ggcacctccg attcaaagct
      601 cagcacggaa gcgcgacgcc tgaagcgccg cgtcgttgcc gcggcggcac gcaaggccga
      661 agcgactcaa tcctgagctg atccagccgc cggcgtcggt cgcggcgccg caacgagagc
      721 ggcgacgcct tccgcgatcg cagaattatc gatgcgccgt acatcgttgc cagcggcggt
      781 gagcgcgctc gcgagagcgc catcggtgag cgtatctttc ccagtgccgc tcggacccat
      841 cacgaggaac aaaataccgc gcgacggcat gctgcgcgct cctccgccaa cgacccgatg
      901 ccgacaaatt taagctttgt gtgccgggca ttcaccccga gttaacggcg ctcatggtac
      961 cgaacatgcg ggctttggcc gatctttgcg ggacgagcgt catggacgag gaatcaatcg
     1021 gtttcttcag cgtgctgctg atcgtgacgg cgatcgcgct gacccatccg ttccagctca
     1081 accgcgtcgt gctcaccctg cccgatatcc gctggcagga catggtcgaa gcgagcgctt
     1141 gcggccccga cgtcgccgcc ggctgcactc agacgccgac accctgacgc tcgcggacgg
     1201 ggcgcccccg tctcattccg cggcgacgcc acgaccggcg cgccgctcga tctccgccaa
     1261 acattgctcg agccgagcac cgtcaggatc ggagagggct gcgctcaacg ccgatgccac
     1321 cgcatcgatg gcggccgctg cgggatagcc gccggcgagc gccggacgga tgatccgcga
     1381 ttcaatttgg cgataattgg cgacgccgcg tgcttgtgta tcgccataac ctttgatcag
     1441 gcgcgcgcat tccgcgacgg cgcgcgccaa ttcgggcgag agggtgacgg catccaggat
     1501 cagctggagc caccgctcga tggccacctg ctcctccgca aaacgatgcg tgcggcggcg
     1561 ccaccgccgc agtttcgcga gaagccgcag ccggaggtac ccgctcaccg aggtgctgcg
     1621 cacctccatt ccccaatagg cgcggtcgag ccagccgcgg cgctcggcaa gcccgaggat
     1681 cggacgagca agaagagacg gcaaaatcga gcacagctcg acgatgccgg gcttgaagaa
     1741 gtcgacgatt gccatcgggt cgtcctcgcc gacgccaagc tcggcgcgga tgcgccgcag
     1801 gcgcgccggg tcggtcttcg cctgggcgac gcggatcacg tcctcgaagg acatgcgaac
     1861 ggcgagctgc cgggcgacct cgcgtaacag ccggccgccg gcggccgtcc gaatgtcggc
     1921 atcgcggatc ggcgcgaggc gttcgagata aagctgggca tagcgcagat cctgatacgc
     1981 gacgaggcgc cgcaatcct
//
LOCUS       DASYNS010000161         4656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_815514, whole genome shotgun sequence.
ACCESSION   DASYNS010000161 DASYNS010000000
VERSION     DASYNS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815514"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VGP50_05105"
     CDS             complement(<1..777)
                     /locus_tag="VGP50_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV8026783.1"
                     /translation="MTETPTKARDYSETLFLPKTDFPMRAGLPQKEPEILARWRKLDL
                     YGRLRAAAKGRPRFILHDGPPYANGNIHIGTALNKILKDVVTRSQQMLGFDSNYVPGW
                     DCHGLPIEWKIEEEYRAKGRDKDKVPVIEFRRECRQFAEHWIGVQSGEFQRLGVVGDW
                     EHYYSTMSHPAEAQIARELMKFAANGTLYRGSKPVMWSVVEKTALAEAEVEYEDYVSD
                     QVWVAFPVVSSNAEGLSGRDAKIIIWTTTPWTLPGNRAIAY"
     gene            927..2027
                     /locus_tag="VGP50_05110"
     CDS             927..2027
                     /locus_tag="VGP50_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV8026784.1"
                     /translation="MEYLRVSHEARHALIINDPRPVDNVGGIWTSVGRAATVGTFLLL
                     FGAFLYVGRVLVLPLMAAAIVALTLAPLVRAGKRRGISPWIMALLLVVVSLGAVAIAA
                     TAMAGPVSEWIGRAPEIGATIKQRLSVFDQPLAALRQLQDALFGKDSGVSVSTPAANF
                     VLPVVAFFTPAVGELLLFFAILLFFLGGQFELRAGIVSLFGNRDAKLRFLKIMNDIER
                     NLAGYLTVVTMINAALGTIVALGAWLLGLPNPVIFGLLAALLNYVPYVGPAAMVVILF
                     GVGLVTFPSLGQALLAPAGLVALTTIEGHFITPAIIGRRLTLNPLMVLLALAFWTWIW
                     GPFGAFLATPLCIIGLVVFNHLFPTDDGKLPD"
     gene            2183..2632
                     /locus_tag="VGP50_05115"
     CDS             2183..2632
                     /locus_tag="VGP50_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026785.1"
                     /translation="MLKGWMHDITLAIQARSGASAALFVWMAIIAFASLSAFVFLCIA
                     AYDWLSLQLGGVLAGLIMAGIFVLIAAIGAIFSALARRRARERAILERAARARASSSW
                     LLDPKIVTVAVEAGRALGWQRILPVALLGLMAAQWTREHRAHREERA"
     gene            complement(2641..3600)
                     /locus_tag="VGP50_05120"
     CDS             complement(2641..3600)
                     /locus_tag="VGP50_05120"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV8026786.1"
                     /translation="MAAKRFRVVRDVAPDDALRGAVVAIGNFDGVHRGHRAVIGAAIA
                     RAKALGRPAAALTFEPHPRAFFNPNEPLFRLTDEAGKLRLLASTGLDGAIVLTFDAAL
                     ARLTAEEFVERILLERFAVSGAAIGFNFHFGARRAGSPQFLAAEGRKRGFAVDIVPRF
                     EDDGRPVSSGPIREALAGGRVREATELLGFPWFVSGTVIHGDKRGRELGFPTANLRLD
                     AGCGLRHGIYAVRAAIDGRRYDGVASFGRRPMFDSGAVLLEVYLFDFSGDLYGRSIDV
                     AFIDWIRDEAVFASVEELTRQMQDDARRAREALARAGDVFPPI"
     gene            3854..4264
                     /locus_tag="VGP50_05125"
     CDS             3854..4264
                     /locus_tag="VGP50_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8026787.1"
                     /translation="MALDPSMSVLVVDDYSTMVRIIRNLLRQLGFADIDDANNGEMAL
                     AKMHSKRYGLVISDWNMEPMTGYDLLRQVRADPSLQQTPFIMVTAESKTENVIAAKKA
                     GVNNYIVKPFNAQTLKAKIEAVFTEREREEAPAQ"
     gene            complement(4296..>4656)
                     /locus_tag="VGP50_05130"
     CDS             complement(4296..>4656)
                     /locus_tag="VGP50_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEV8026788.1"
                     /translation="RGDTLVYCAGALADAYAELGGEVLYCGKPYAPIYQAALANAAAL
                     RGGVPPPLARVLAIGDSVRTDLKGAAAFGIDCLFVISGVHAEEFGGRETPDPSSLNAI
                     FAAAGVTPKAIARRLEW"
BASE COUNT          760 a         1583 c         1490 g          823 t
ORIGIN      
        1 atacgcaatc gcgcgattgc ccggcagcgt ccaaggcgtt gtggtccaga tgataatctt
       61 cgcgtcgcgg ccactgaggc cctcggcgtt gctactgacg accggaaatg caacccacac
      121 ctggtccgag acataatcct catactcgac ctcggcctcg gcgagcgcgg tcttctccac
      181 caccgaccac atcaccggct tcgagccgcg atagagcgtg ccattcgctg cgaacttcat
      241 cagctcgcgc gcgatctgcg cctcggctgg atggctcatg gtgctgtaat agtgctccca
      301 gtcgccgacc acgcctaagc gctgaaactc cccgctctgt acgccgatcc aatgctcggc
      361 gaattgccgg cactcgcggc ggaattcgat caccggcacc ttgtccttgt cgcgaccctt
      421 ggcgcggtac tcctcctcga tcttccattc gatcggcagg ccgtggcagt cccagccggg
      481 cacgtagttc gaatcgaagc cgagcatctg ctggctcctc gtcaccacgt ccttgaggat
      541 cttgttgagc gcggtgccga tatggatgtt gccgttggcg tagggcgggc cgtcgtggag
      601 gatgaagcgc ggccggcctt tcgccgccgc gcgcaaccgg ccgtaaaggt cgagcttgcg
      661 ccagcgcgcg aggatctccg gctccttctg cggcaggccg gcgcgcatcg ggaaatcggt
      721 cttgggcagg aacagcgtct cgctgtaatc gcgcgctttt gtcggcgtct cggtcatggc
      781 gcgccttcta acaagccgct tgcgccgcgc aaagggctct gccgcctagg cgaccgccct
      841 cgaaggaacc gccggcctgc ggggcgcgtt gacactatgg gaccgtggct tcgcgacggt
      901 tggcgaggga aagataagga aagtccgtgg aatatctccg cgtgagccac gaagcccgcc
      961 acgctctcat catcaacgac ccgcggccgg tcgacaatgt cggcggcatc tggacgtccg
     1021 tcggccgcgc cgcgaccgtc ggcaccttcc tcttgctgtt cggcgccttc ctctatgtcg
     1081 gccgcgtgct cgtgctgccg ctcatggcgg cggccattgt cgccctgacg ctcgcccctc
     1141 tggtcagggc cggcaaacgc cgcggcattt caccctggat catggccctc ctcctcgtcg
     1201 tcgtcagcct gggtgcggtc gccatcgccg cgacggcgat ggccggcccg gtgagcgaat
     1261 ggatcggtcg cgctccggag atcggcgcca ccatcaagca gcggctctcg gtattcgatc
     1321 aaccgctcgc ggcgcttcgc caattacagg acgccctgtt cggcaaggac agcggggtca
     1381 gcgtcagcac gcccgcggcc aattttgtct tgccggtggt ggcgttcttc accccggccg
     1441 tcggcgagct cctgctgttc ttcgccattt tgttgttctt cctcggcggc cagttcgaat
     1501 tgcgcgccgg catcgtctcg ttgttcggca accgcgacgc caagctgcgt ttcctcaaga
     1561 tcatgaacga catcgagagg aatctcgccg gctacctgac cgtagtgacg atgatcaacg
     1621 ccgcgcttgg gacgatcgtc gcgctcggcg cgtggctcct cggcctgccc aatccggtga
     1681 tcttcggcct gctcgccgcg ctgttgaact acgtgcctta tgtcggaccg gcggccatgg
     1741 tcgtgatctt gttcggggtt ggcctcgtca cctttccctc gctcggccag gcgctccttg
     1801 ctcccgcggg actcgttgcg ctcaccacca tcgagggaca cttcatcacg ccggcgatca
     1861 tcggccggcg gctgacgctc aacccgctca tggtgttgct ggcgctcgcc ttttggacct
     1921 ggatttgggg gccgttcggc gccttcctgg cgacgccgct ctgcatcatc ggcctcgtcg
     1981 tctttaatca tctattcccg accgacgacg gtaaacttcc cgattgaatg gaacttcgcc
     2041 gctcccggac cattgtcagg gcgacttgag cgttcacccg gcatgggaga gcaagcatga
     2101 acaccggatc gacggtcgca cagatcattg gccaagcctt cacgtggcgc cggtaagggc
     2161 cgctgagccg cgcgagagac cgatgctcaa gggctggatg cacgatatta ccctggcgat
     2221 ccaggcgcga agcggcgcca gcgccgctct ttttgtttgg atggcgatca tcgcgttcgc
     2281 atcgctgagc gccttcgtgt ttctttgcat tgccgcttac gattggctgt cgctgcaatt
     2341 gggcggcgtc ctcgccggcc tgatcatggc cggaatcttc gtcctcattg ccgcgatcgg
     2401 cgccatcttt tcggcgctcg cccgccgccg cgccagggag cgcgcgatcc tcgaacgcgc
     2461 cgcgcgggcg cgggcctcgt cgtcctggct gctcgatccg aagatcgtta ccgtcgcggt
     2521 cgaggccggc cgggcgcttg gctggcagcg catcctgccg gtcgccctcc tcggcctgat
     2581 ggcggcgcaa tggacgcgcg agcatcgggc ccaccgcgaa gagcgggcct gagccattcc
     2641 tcagatcggc gggaagacgt cgccggcccg ggccagcgct tcgcgggcgc ggcgcgcatc
     2701 gtcctgcatc tgccgtgtga gctcctcgac tgaagcgaac accgcctcat cgcggatcca
     2761 gtcgatgaac gccacgtcga tgctgcgacc atagaggtcg ccggagaaat caaagagata
     2821 aacctcgagc aagacggcac cgctgtcgaa catcggccgc cggccgaagc tcgctacgcc
     2881 gtcatagcgg cgtccatcga tcgcggcgcg caccgcatag atgccgtgcc gcagcccaca
     2941 accggcgtcg agccgcaggt tcgccgtcgg gaagccgagc tcgcgcccgc gcttgtcgcc
     3001 gtggatcacc gtgccggata cgaaccaggg aaaacccagg agctcggtcg cctcgcgcac
     3061 gcgtccgccg gcaagcgcct cgcgaatggg cccggaggaa accggacgcc cgtcgtcctc
     3121 gaaccggggc acgatatcca ccgcgaagcc gcgcttgcgg ccctccgccg cgagaaattg
     3181 cggcgatccg gcgcggcgcg cgccgaaatg aaaattaaag ccgattgccg cgccgctgac
     3241 cgcgaaccgc tcgagcagaa tccgctcgac gaactcctcg gcggtgaggc gggcaagcgc
     3301 cgcgtcgaag gtgagcacga tcgcgccgtc gaggccggtc gaggcgagga gccgcagctt
     3361 gccggcctcg tcggtcaggc ggaacagcgg ctcgttcggg ttgaagaagg cgcgcggatg
     3421 cggctcgaaa gtcagcgccg ccgccggccg gcccagcgcc ttggcgcggg cgatggcggc
     3481 gccgatcacg gcgcggtggc cgcggtgcac gccgtcgaaa ttgccgatgg cgacgaccgc
     3541 gccccgcaag gcgtcatcgg gcgcgacatc gcgcaccacc cggaaacgct tggcagccat
     3601 cgggctccaa aggttaagag aaacctaccg gcgtaggtgc cgccgcgtcc gagccaagtc
     3661 aagcaacgcg gggcctcgat tgcgtgccgc ggcggggccg ggttcccgcg attaactcga
     3721 tgttcaacac cggggtgata ttttttcacc attcgtctgg gatgggagag cctcgggagc
     3781 ccttcccggg agaggagccg accaagctcc gcttcgcgcc attcggcgtg cgcgccaggg
     3841 ggagcagagg gcgatggctc ttgatccgtc aatgtcggtg ctggtggtcg acgactacag
     3901 cactatggtc cgcatcatcc gcaatctgtt gcgccaactt ggtttcgccg acatcgacga
     3961 cgccaataat ggcgaaatgg cgctcgccaa gatgcactcc aagcgttacg ggctggtgat
     4021 ctccgactgg aacatggagc cgatgaccgg ctacgacctg ctgcgccaag tgcgcgccga
     4081 tccgagcctg cagcagacgc cgttcatcat ggtcaccgcc gaatcgaaga ccgaaaacgt
     4141 catcgcggcg aagaaggcag gcgtgaacaa ctacatcgtc aagccgttca acgcccagac
     4201 gctcaaggcc aagatcgagg cggtgttcac cgagcgcgag cgcgaggaag ctcccgcgca
     4261 gtaatcgtct ttccgctcat tccgagctgc gccttctacc attcgagccg ccgcgcgatc
     4321 gccttcggcg tgacgcccgc ggccgcgaag atcgcgttca ggcttgaggg atcgggcgtt
     4381 tcgcgcccac cgaattcttc ggcatggacg ccggagatca cgaacaggca atcgatgccg
     4441 aaggccgccg cgcctttgag atcggtgcgc accgaatcgc cgatcgccag cacccgcgca
     4501 agcggcggcg gcacgccgcc gcgaagcgcc gcggcgttgg cgagcgcggc ctgatagatc
     4561 ggcgcgtagg gcttgccgca atagagcacc tcgccgccga gctccgcata ggcgtcggca
     4621 agcgcgccgg cgcaatagac cagggtgtcg ccgcgc
//
LOCUS       DASYNS010000162         8859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1907436, whole genome shotgun sequence.
ACCESSION   DASYNS010000162 DASYNS010000000
VERSION     DASYNS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907436"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /gene="gltD"
                     /locus_tag="VGP50_05135"
     CDS             <1..195
                     /gene="gltD"
                     /locus_tag="VGP50_05135"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727224.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HEV8026789.1"
                     /translation="LGVARDPRGNVKANERDYATSVSKMFVAGDARRGQSLVVWAIRE
                     GRQCARAVDEFLMGRSDLPR"
     gene            289..1590
                     /locus_tag="VGP50_05140"
     CDS             289..1590
                     /locus_tag="VGP50_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEV8026790.1"
                     /translation="MAKRAPEPRVVNPEDIDPRHNWERPILALGPSAVDFEERIDFRR
                     LHRYRLGRTRQALAKSGLGALLVFDQYNIRYMTSTVIGNWARDKMTRYALLPGDSEPH
                     VWDFGSAAKHHRLYAPWLPQHNCHAGMLGLRGAVHPKIGLMRKAAEEIKSILDENGVG
                     NMPVGIDVVEPAMMFELQAAGIELRDGQQVMLDARELKSADELALLNMSAALVDGTYQ
                     TIAEHLKPGIRENEIVALANKRLYELGSDDVEAINAISGERCSPHPHNFTDRLIRPGD
                     QAFFDIIQSFMGYRTCYYRTFNVGRATDAQRDAYKRAREWIDASIALVKPGVTTDKIA
                     ACWPKAQDIGFASEMEAFGLLFGHGLGLGLHERPIISRLNSFEDPMEIKEGMVFALET
                     YCPASDGYSAARIEEEVIVTPKGAQVITLYPAEELPIANPY"
     gene            1603..2355
                     /locus_tag="VGP50_05145"
     CDS             1603..2355
                     /locus_tag="VGP50_05145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8026791.1"
                     /translation="MELKGTVALVTGGNGGLGQRICHALAKEGVHIAVVYAQSRAQAD
                     GVARELASRYRINAAAFGCDITDGAAIDALIGRVLERFGRFDILVNDAAYNKSVPFAD
                     LDNLTLELWDKIMAVNLTGPLRLIKAAAPVMKAQKQGRIVNISSVAGLGPTGSSIAYA
                     VSKAGLNHLTRCMALALAPETLVNCVAPGLLEGTRATANLAPEHLERATSGALLKKAA
                     DKDDCAAMVVAMCRTETMTGQTVVIDAGRIFH"
     gene            complement(2425..3765)
                     /gene="dctA"
                     /locus_tag="VGP50_05150"
     CDS             complement(2425..3765)
                     /gene="dctA"
                     /locus_tag="VGP50_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HEV8026792.1"
                     /translation="MAEMSLTRSQTPKAANARPAYRKLYVQVLAAMLIGALLGALDPE
                     VAVALKPLGDAFIKAIRALIAPIVFLTVVVGIARIGDMRRVAVVGVKSLIYFEIVSTL
                     ALLIGLAVASLYHIGAGINADPTVLNAKLAAGVAESAKPPTLIDFLLAIIPASFVGAF
                     TSGEILPVLFLAILFGLALCQLGARVTPLIDLLDQGVQGLFGVVRIIMYAAPVGACGA
                     IAFTIGKYGVHTLLQLGQLVAGVYVVSILFVVVVLGGALRLAGFDLWRVLGYFKDEIL
                     FVFCATSAETMIPRSMAKLEELGCSKEVVGLVMPAGFAFNMDGTAIYMAMAVLFIAQA
                     TNIDLSLTQQLTILFVMLFTSKGAAGVAGGGFVALAATMPAIDVLPIGGLALLLGVDR
                     FMAEIRAATNLASNVIATLVVARWSGAIDMAQARDALYGDLEAVPKTGRAAAGD"
     gene            complement(3774..4889)
                     /locus_tag="VGP50_05155"
     CDS             complement(3774..4889)
                     /locus_tag="VGP50_05155"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV8026793.1"
                     /translation="MTAQSGSNAFRIPDKMKAWVLGKPDEIHFTEKPVPEPGAAEVLV
                     RIDAVAICATDLEIISHGVPAKIGGGLPFEKNFTPGHEYMGTVVKLGPAVDEYDLGDR
                     VTVEVHAGCGRCERCRQGMYTSCLNYGEPQKGHRANGFTTDGGFAEYAINHINTLVHV
                     PDDMTDAEATLVVTAGTSMYGLDVLGGLIAGEGVVISGPGPIGLLGVAVAKALGADPV
                     ILTGTRDARLDLGRKLGADHVVNIRREDPVAAVRQLTGGKGVHFVVECSGAPEAMNQA
                     AQMVNRGGRICLAAFPHEPVAVDLAHLVRNNIYVYGIRGEGQSATHRAMALMKQKRFD
                     AKLIHTHSFALDELPTALKYARERIEDAIKVVVTIRH"
     gene            4995..5930
                     /locus_tag="VGP50_05160"
     CDS             4995..5930
                     /locus_tag="VGP50_05160"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8026794.1"
                     /translation="MAPELKRHKLGWIGVGRMGYPMAERLARAGADVSVYNRTRAKAQ
                     PLAKAGAALVDRPADLADRDIVFTMIAGSDDLKQVTVGKDGVLTSPGKAPRILIDCST
                     VSEESSAEVRTAAAKLGTQMLAAPVSGNGKVVKAGKLSLVVSGPRDAYETALPYLERL
                     GQGVSYVGDGELARMVKICHNVFLGILIQGLVEVTVLAQKGGVPRHAFLDFINKSVLG
                     STFTRYKTPALVNLDFAPTFTPVLLRKDLDLGLAAARKAGAPMAVTALVREILQGLIG
                     EGFVDEDFATLLVQQARAAGMPLEPENVAIADGLD"
     gene            complement(6063..7559)
                     /locus_tag="VGP50_05165"
     CDS             complement(6063..7559)
                     /locus_tag="VGP50_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEV8026795.1"
                     /translation="MLVTSVRRAVPVFGLAAFVVGCAVGPDFMRPSAPETKDYEAGGM
                     PQPDVPEPTETQQRFAVGKKISNDWWQLFHSSRLESVLEEAIAGSQTLAAAKATLAQA
                     QQGVLQAAGALYPQVDLAAGAQRQRVNFAAIGEPVEGPIINLFNIGPTVSYSVDVFGR
                     YRRQVEEQQALAEYQAWELDAAYLTLTGNAATQAFQIASVRAQIAAVQTIIADDERNL
                     GLVQTELRAGEATQIDVQSAQSQLAADRTLLPPLRQQLSVARHALSVLSGRAPGDWMP
                     PDFDLAEFTLPEDLPVSLPSELVRQRPDILAAEAQLHAASAAVGVATAQLYPSIDLTA
                     SFEQFATKPSKLFVPASSVFDLAGNLVAPIFHGGTLRAQKKAAVDAFDGAYATYRQTV
                     LTSFGQVADSLQALSHDAELLEAEHRALDSAEANLRLTRTTYSYGNVGILQVLDAQRL
                     AEQARLGYVRAQAQRYLDTVELLTAMGGGWWQSQDQTALAVTPGKAPR"
     gene            7773..8360
                     /locus_tag="VGP50_05170"
     CDS             7773..8360
                     /locus_tag="VGP50_05170"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV8026796.1"
                     /translation="MDDDHIEKWRRQERRRLLAERLTVRPEQHRRWSAAIESRLSPLL
                     NGLPGTVIGLYWPVRAEFDARPLAAGLRERGRMTALPAVIERGGRLEYRIWEGDAEVI
                     EGLYGIPVPKERWLANPGIVVVPVVGFDAANYRLGYGGGYFDRTLAALEPRPLAVGVG
                     FEAARLETVFPRSHDIAMDIVVTEACLQRKTGAEQ"
     gene            8357..>8859
                     /locus_tag="VGP50_05175"
     CDS             8357..>8859
                     /locus_tag="VGP50_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEV8026797.1"
                     /translation="MTRAPEISVAELLAERVSGLSPAKLPLTLRHVIDDLVLDVVGLC
                     LAARQTEYVSAALAGADGDGACTVIGHTRQGSATTAALINGIAAHGEDFDDTFEGGPV
                     HAGAVVVPAVLAIAERFRPEGPAALLGIAVGIETICRLSLVAPTRVHDAGFHPTSVFG
                     AVAAAIAA"
BASE COUNT         1587 a         2887 c         2935 g         1450 t
ORIGIN      
        1 ctcggcgtcg cgcgcgatcc gcgcggcaac gtcaaggcga acgagcgcga ctacgcgacc
       61 tcggtgtcga aaatgttcgt cgcgggcgac gcgcggcgcg gccagtcgct cgtggtctgg
      121 gccatccgcg aaggccgtca atgcgcccgc gcggtggacg agttcctcat gggacgatcg
      181 gacctgccgc ggtgatatga tggtgtggcg tcgcccctca ccctcccagc gcttcgcgct
      241 gggtccctcc ctctcccaca aagggagagg gtaagagtac ggccgcagat ggcgaagcgc
      301 gctccggaac ccagagtcgt caatcccgag gatatcgacc cgcggcacaa ctgggagcgg
      361 ccgatcctgg cgctcggacc gagcgcggtc gatttcgagg agcgcatcga tttccgccgg
      421 ctgcaccgct atcgcctggg ccgcacgcgc caggcgctgg cgaaatcagg gctgggcgcg
      481 ctcctcgtat tcgaccagta caacatccgg tacatgacga gcaccgtcat cggcaactgg
      541 gcgcgggaca agatgacccg ctacgcgctg ctgccgggcg acagcgagcc gcatgtctgg
      601 gatttcggtt cggcggcgaa gcatcaccgg ctctatgcgc cctggctgcc gcagcataat
      661 tgccacgcgg gcatgctggg cttgcgcggc gccgtccacc ccaagatcgg cctcatgcgc
      721 aaggcggccg aggagatcaa gtcgatcctc gacgagaacg gcgtcggcaa catgccggtc
      781 ggcatcgacg tcgtcgagcc agccatgatg ttcgagctgc aagcggcggg gatcgagctg
      841 cgcgacggcc agcaagtcat gctcgatgcg cgcgagctca agagcgcgga cgagctcgcg
      901 ctcctcaaca tgtcggcggc gctcgtggac ggcacctacc agaccatcgc cgagcatctg
      961 aagccgggca tccgcgagaa cgagatcgta gcgctggcca acaagcggct ttacgagctg
     1021 ggctccgacg acgtcgaggc catcaacgct atctccggcg agcgctgcag cccgcatccg
     1081 cacaatttca ccgatcgcct catccgcccc ggcgaccagg ccttcttcga catcatccag
     1141 tccttcatgg gttatcgcac ctgctactac cgcaccttca atgtcgggcg cgccacggac
     1201 gcccagcgcg atgcctacaa gcgcgcccgc gaatggatcg atgcgtcgat cgcgctggtg
     1261 aagccaggcg tcaccacgga caagattgcg gcgtgctggc cgaaggcgca ggacatcggc
     1321 ttcgccagcg agatggaggc gttcggcctg ctcttcggcc atggcctggg actcggactc
     1381 cacgagcgac ccatcatcag ccggctcaac tccttcgaag atcccatgga gatcaaggaa
     1441 ggcatggtct tcgcgctcga gacctattgc ccagcaagcg acggctattc ggcggcgcgc
     1501 atcgaggaag aggtgatcgt aacgcccaaa ggggcgcagg tgatcaccct ctatcccgcc
     1561 gaggagctgc cgatcgccaa tccttattga ggggcgaagc tcatggagct taagggaacg
     1621 gtcgcgctgg tgactggcgg caatggcggg ctcgggcagc gcatctgcca tgcgctggcg
     1681 aaggaaggcg tgcatatcgc cgtcgtttac gcgcagagcc gcgcgcaggc ggacggcgtg
     1741 gcccgcgaac tcgcatcgcg atatcggatc aatgctgcgg cgttcggctg cgacatcacc
     1801 gacggcgcag ctatcgatgc gttgatcggc cgcgtgctgg agcgcttcgg gcgtttcgat
     1861 attctcgtca acgacgccgc ctacaacaaa tcggtcccct ttgccgacct cgacaatctg
     1921 acgctcgagc tgtgggacaa gatcatggcc gtcaacctga cggggccgct ccgcctcatc
     1981 aaggcggcag cgccggtgat gaaggcgcaa aagcagggcc gcatcgtcaa catctcgtcg
     2041 gtggccgggc tcgggcccac gggctcgtcc atcgcctatg ctgtgtcgaa ggcgggcctc
     2101 aaccacctca cccgctgcat ggcgctggcg ctcgcgcccg agaccctggt caactgcgtc
     2161 gcgccaggac tgctcgaagg cacgcgcgca acggccaatc tggcgcctga gcacctcgag
     2221 cgcgcgactt cgggagcgct cctgaagaaa gctgccgaca aggacgactg cgctgcgatg
     2281 gttgtcgcca tgtgccgcac cgagaccatg accggccaga ctgtcgttat cgacgccggg
     2341 cgcatctttc actgagagtc cgacgcatcg gcagcggcgc ttcatcgtcg tcccgcgaaa
     2401 gcgggcaagc gacatggagg tgctctaatc gccggccgcg gcgcgccccg tcttgggcac
     2461 ggcttcgaga tcgccataaa gcgcatctcg ggcttgagcc atgtcgatcg cgcccgacca
     2521 gcgcgccacc accagcgtcg cgatcacgtt gctcgcgaga ttcgtggcgg cgcggatctc
     2581 agccatgaag cgatcgaccc ccaggagcag cgccaagccg ccgatcggca ggacgtcgat
     2641 tgccggcatg gtggcggcga gcgcgacgaa gccgccgccg gcgacaccgg cggcaccctt
     2701 cgaggtgaac agcatcacga agaggatggt gagctgctgc gtcaagctga gatcgatgtt
     2761 ggtggcctgg gcaatgaaca ggaccgccat ggccatgtag atcgccgtcc cgtccatgtt
     2821 gaaggcgaag cctgccggca tcaccaagcc caccacctct ttcgagcaac cgagctcctc
     2881 gagcttggcc atgctgcgcg ggatcatggt ctcggccgag gtggcgcaga agacgaacag
     2941 aatctcgtcc ttgaaataac caagcacgcg ccacaggtcg aagcccgcca gccgaagcgc
     3001 gccgccgagc acgacgacga cgaagagaat gctcacgaca tagacgccgg ccacgagctg
     3061 tccgagctgg agcagcgtgt gaacgccgta tttgccgatg gtgaaagcga tggcgccgca
     3121 cgcgccgacc ggagctgcgt acatgatgat gcgcacgacg ccgaacaatc cctggacgcc
     3181 ctgatcgagc agatcgatca acggcgtgac gcgcgcaccc aattggcaaa gagcgagacc
     3241 gaacaggatg gcgaggaaca gcaccggcag gatctcgccc gatgtgaagg cgccgacgaa
     3301 actggcgggg atgatggcca agaggaaatc gatgagcgtc ggcggcttcg cgctctccgc
     3361 tacgcccgcc gcgagcttcg cgttcaagac cgtggggtcg gcgttgatgc ccgcgccaat
     3421 atggtagaga ctggcgacag cgaggccgat gagcagcgcc agcgtcgaca cgatctcgaa
     3481 atagatcagc gatttgactc cgaccactgc gacgcgtcgc atgtcgccga tgcgggcgat
     3541 gccgactacc accgtgagga agacgatggg agcaatgagc gcgcggatcg ccttgatgaa
     3601 cgcgtcgccc aacggcttga gcgcgaccgc gacctccgga tcgagagctc cgagcagcgc
     3661 tccgatcagc atcgctgcga ggacctggac atagagtttg cgatacgccg ggcgggcgtt
     3721 ggccgccttc ggcgtctggg accgcgtcag cgacatctcg gccatggcgc cgctcaatgc
     3781 cggatcgtga cgacaacctt gatcgcgtcc tcgatgcgct cgcgcgcata tttgagcgcg
     3841 gtcggcaact catcgagagc gaagctgtgg gtgtggataa gcttggcatc gaagcgcttc
     3901 tgcttcatca aagccatggc gcgatgcgtg gcgctctgcc cttcgccccg gatgccatag
     3961 acataaatgt tgttgcgaac gaggtgggcg agatcgactg cgacgggctc atgcggaaag
     4021 gcggcgagac agatccgccc gccgcggttg accatctgcg ccgcctgatt catcgcctcg
     4081 ggagcgcccg agcattcgac gacgaagtga acacctttgc cgccggtcag ctggcgaaca
     4141 gcagcaaccg gatcttcgcg gcgaatattg acgacgtgat ccgcgccaag tttccggccg
     4201 agatcgagcc gggcgtcgcg cgttccggtg aggatcacag gatcagcgcc cagcgccttc
     4261 gcaaccgcga cgcccaggag gccgatcggc cccggacccg atatgacgac gccttcgccg
     4321 gcgatcagtc caccgagcac gtcgagccca tacatcgagg ttccggcggt gacgaccagc
     4381 gtcgcttccg catccgtcat gtcgtctggc acgtgcacga gcgtattgat gtgattgatc
     4441 gcgtattcgg cgaagccgcc gtcggtggtg aagccgttgg cgcggtgtcc tttctgcggc
     4501 tcgccgtagt tgaggcagga ggtatacatc ccctggcggc agcgctcgca gcgcccgcat
     4561 ccggcatgca cttcgacggt gacgcgatcg ccgagatcgt attcgtcgac ggccggcccg
     4621 agcttgacga cggtgcccat atattcatgg ccgggcgtga aattcttctc gaagggcaga
     4681 cccccgccga tcttcgccgg gacgccgtgg ctgatgatct caaggtcggt ggcgcatatc
     4741 gcgactgcgt cgatgcgaac caggacttcc gccgcgccgg gctccggcac cggcttctcg
     4801 gtgaaatgga tctcgtcggg cttgccgagc acccaggcct tcatcttgtc cgggatccga
     4861 aacgcgttgc tgccgctttg ggccgtcatt ttcctgctcc cacgtctcgc tccaacgcgt
     4921 tgcgccccca gtgcggatac gctacagccc gccgcgtttg cttgtaaagg aaggcgtatc
     4981 acggagaagt cgtcatggca ccggaactga agcgacacaa gctcggctgg atcggcgtcg
     5041 gccgcatggg ttatccgatg gcggagcgtc tggccagggc cggagccgat gtcagcgtct
     5101 acaatcggac gcgcgccaag gcgcagccgc tcgccaaggc aggggccgca ctggtggatc
     5161 ggccggcaga tctagcggat cgcgatatcg tcttcacgat gatcgcggga tccgacgatc
     5221 tgaagcaggt gacggtcggg aaggacggcg tgctcacgag ccccggcaag gcgccacgca
     5281 tcctcatcga ttgctcgacc gtatccgaag agtcatcagc cgaggtgcgg acggccgcgg
     5341 caaagctcgg cacacagatg ctcgcggcac cggtgagcgg caacggcaag gtcgtgaagg
     5401 ccggcaagct gagcctcgtc gtctccggac cgagagacgc ctacgagacg gcactgccct
     5461 atctcgaaag gctcggccaa ggcgtgagct atgtcggcga cggcgagctc gcgcgcatgg
     5521 tcaagatctg tcacaacgtc tttctcggca tcctcatcca aggactggtc gaggtcaccg
     5581 tgctggcgca aaagggcggc gtgccgcgcc atgcctttct cgacttcatc aacaaaagcg
     5641 tgcttggctc gaccttcacc cgctacaaga cgccggcgct cgtcaatctc gacttcgcgc
     5701 cgaccttcac gccggtgctg ttgcgcaaag acctcgacct ggggctcgcc gcggcgcgca
     5761 aggccggcgc gccgatggcg gtaacggcgc tcgtgcgcga gatcctgcag ggtctcatcg
     5821 gcgagggctt tgtcgatgag gatttcgcga cgctgctggt gcagcaggcg agagccgccg
     5881 gcatgccgct cgagccggag aacgtcgcga ttgctgacgg actcgactga ggcagcggat
     5941 ttgcgcttcg ggcccgctca cctgcgtctt aaagcagcgt gacaccctac gggcggcggg
     6001 cttgcatgac ggccgccgat cgatgcccac tcccggtcct cctcacgcct gatcaccctc
     6061 tgtcagcgcg gggccttgcc gggggtcact gccagcgccg tctggtcctg gctctgccac
     6121 cagccgccgc ccatggcggt cagcagctcg acggtgtcga gataacgctg cgcctgtgcg
     6181 cggacatagc ccagacgcgc ctgctcggcg aggcgctggg cgtcgagcac ctgcaggatt
     6241 ccgacattgc cgtagctgta agtcgtgcgg gtgaggcgga ggttggcttc ggccgaatcg
     6301 agtgcgcgat gctccgcctc cagcagctcc gcgtcatggg agagcgcctg gagagagtcg
     6361 gcgacctgcc cgaaggaggt gagcaccgtc tgcctgtacg tggcgtaggc cccgtcgaaa
     6421 gcatcgaccg ccgccttctt ttgggcgcgc agagtgccgc catgaaagat cggagccacg
     6481 agattgccgg cgagatcaaa gacgctgctt gccggcacga agagcttcga tggcttggtg
     6541 gcgaattgct cgaaggacgc ggtcaggtcg atgctcggat agagttgcgc cgtcgccacg
     6601 cccacggctg cgcttgccgc atggagctgg gcttccgcgg cgagaatgtc cggacgctgg
     6661 cgcacgagct ccgagggaag gctgaccggc agatcctccg gcaaagtgaa ctcggcgagg
     6721 tcgaaatcgg gcggcatcca atcgcccggc gcccgccccg acagtacaga caaggcgtgg
     6781 cgcgccacgc tcaactgctg acgcagcggc ggcagcagcg tgcgatcggc ggcgagctgg
     6841 ctctgggcgc tttgcacgtc gatctgcgtc gcctcgccag ccctgagctc ggtctgcacg
     6901 agcccgaggt tccgctcgtc atcggcgatg atcgtctgca ccgcggcgat ctgcgcccgt
     6961 accgacgcga tctgaaaggc ctgggtggcg gcgttgccgg tgagcgtcag ataggcggcg
     7021 tcgagctccc aggcttgata ttcggccagt gcttgctgct cctcgacctg gcgccgatag
     7081 cggccaaaga catcgacgga atagctgacc gtcggcccga tattgaaaag attgatgatg
     7141 gggccctcga cgggctcccc gattgcggcg aagttgacgc gctggcgctg cgcacccgcc
     7201 gcgagatcga cttgcggata gagggcaccg gcggcctgca gcacgccttg ctgcgcctgc
     7261 gcgagcgtcg ctttcgcggc tgccaaggtc tggctgcccg cgatggcctc ctcgaggacg
     7321 ctctcgagcc gcgacgagtg gaagagctgc caccagtcgt tcgagatctt cttgcccacc
     7381 gcgaaacgct gctgcgtctc cgtcggctcg gggacgtccg gctggggcat tcccccggcc
     7441 tcgtagtcct tggtctccgg cgccgaaggg cgcatgaaat cgggcccgac cgcgcaaccg
     7501 acgacgaacg cggccaagcc gaacacggga acggctctcc ggacagaggt gaccagcatc
     7561 gaatgaactc ccgtcagcga atccgaaaga ggcaatccga cgtcgaagac gtcgaatgcc
     7621 gggcgcggcc ggacggccga cgcccaagat gcgagccggt cgtgcaggct ggcgggcaat
     7681 cgtatcacaa tcgttaccat ctttggcgac gcagaaagga tcgatcattg ccacccccgt
     7741 cgggcatcgc ataaggtccc tcgcgcgact ttatggacga cgatcacatc gaaaaatggc
     7801 gacgccagga gcggcgtcgc cttctggcgg aacggctgac cgtccgcccc gagcagcatc
     7861 gccgctggtc cgccgcgatc gagagccgac tttcgccgtt gctcaacggg ctgccgggga
     7921 cggtcatcgg cctttactgg cccgtcaggg ctgaattcga tgcgcggccg ttggcggctg
     7981 gcctcagaga acgcggacga atgacggcac tccccgccgt gatcgagcgc ggcggccggc
     8041 tcgagtatcg gatatgggaa ggcgacgcgg aggtgatcga agggctctat ggcattcccg
     8101 tcccgaaaga gcgctggctc gcgaacccag gcatcgtcgt cgtgccggta gtgggattcg
     8161 acgccgcgaa ttatcggctc ggctatggcg gcggctattt cgatcggacg ctcgccgccc
     8221 tcgagccacg tcctctcgcc gtcggcgtgg ggttcgaggc ggcgcgtctc gagacggtct
     8281 ttccgcgctc tcacgacatc gccatggaca tcgtcgtgac cgaagcatgt ctgcaacgga
     8341 aaacgggcgc cgagcaatga cacgggcgcc agaaatcagc gttgccgagc tgctggcgga
     8401 acgggtttcc gggctgtccc cggcaaagtt gcccctgacg cttcggcacg tgatcgacga
     8461 cctcgtgctc gatgtcgtcg gtctctgcct ggcggcgcgg cagacggagt atgtgagtgc
     8521 cgcgctagcg ggcgcagacg gcgacggcgc ctgcactgtc atcggccaca cacggcaagg
     8581 cagcgccacc acggccgcgt tgatcaacgg catcgccgcc catggcgaag atttcgacga
     8641 tacctttgag ggcggtccgg tgcatgccgg ggccgtggtg gtcccggcag tgctcgcgat
     8701 cgccgagcgc ttccgtccgg aaggcccggc ggcgcttctc ggcatcgctg tgggcatcga
     8761 gacgatctgc cgcctcagcc tcgtcgcgcc cacgcgcgtc cacgacgccg gctttcatcc
     8821 gaccagcgtc ttcggtgcgg tggcggcggc gatcgccgc
//
LOCUS       DASYNS010000163         1598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2220659, whole genome shotgun sequence.
ACCESSION   DASYNS010000163 DASYNS010000000
VERSION     DASYNS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220659"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VGP50_05180"
     CDS             <1..1151
                     /locus_tag="VGP50_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HEV8026798.1"
                     /translation="KANAGHVSLAQLKVMSAIETCRTAALGGHVERCEDCAHERVAYN
                     SCRNRHCPKCQAAAAWQWLEDREAELLPVPYYHVVFTLPAAIGAVAFQNKAAVYDLLF
                     RTAAETLTTIAADPKHLGARIGLTAVLHTWGSALTHHPHIHVIVPGGGLSPDGSRWIA
                     CKPGFFLPVRVLSRLFRRLFLEGLAALHAAGRLVFLGDLAPLGEKSAFDTALAPLRRA
                     EWVVYAKRPFAGPQAVLAYLARYTHRVAISNARLIALNEKGVTFKWKDYRIKGRDRLK
                     TMTLAAHEFIRRFLLHVLPSGFHRIRHYGLIAGALRADNIERARQLLAAARAAPQRPC
                     AEADSHAEDVSPARRCPCCGGRMIIVETFEGSRPWRSPSPSRIRIDSS"
BASE COUNT          238 a          647 c          467 g          246 t
ORIGIN      
        1 gaaaggccaa cgccggtcac gtgagcctcg cccagctgaa ggtcatgtcg gcgatcgaga
       61 cctgtcgcac agccgcgctc ggcggccacg tcgagcgctg cgaggactgc gcgcacgagc
      121 gcgtcgcata caactcctgc cggaaccgtc actgcccgaa gtgccaggcg gccgccgcgt
      181 ggcaatggct cgaagaccgc gaggcggagc tcctgccggt tccctactat cacgtcgtct
      241 tcaccctgcc ggccgcgata ggcgccgtcg cgttccagaa caaggcggcc gtctacgacc
      301 tcctgttcag aaccgccgcg gagacgctga ccacgatcgc cgccgacccg aagcatctcg
      361 gcgcgcgcat cggcctgacc gccgtactgc acacctgggg ctcggcgctc acccaccatc
      421 cgcacatcca cgtcatcgtt cccggcggcg gcctgtcgcc ggacggttct cgctggatcg
      481 cctgcaagcc cggcttcttc ctgcccgtgc gggtgctgtc gcgtctcttc cggcggctct
      541 tcctcgaagg gctcgcggcc ctgcatgcgg ccggccgcct cgtcttcctc ggcgacctcg
      601 cgcccctcgg cgagaagagc gccttcgaca ccgcgctcgc gccgctgcgc cgcgccgagt
      661 gggtggtcta cgccaagagg ccgttcgccg ggccgcaggc cgtcctcgcc tatctcgccc
      721 gctacaccca ccgcgtcgcg atctcgaacg cccggctcat cgcgctcaac gagaagggcg
      781 tcaccttcaa atggaaggac taccgcatca aaggacgcga ccggctcaag accatgacgc
      841 tcgcggccca cgagttcatc cgccgtttcc tcctgcacgt gctgccgagc ggcttccacc
      901 gcatccgcca ctacggcctc atcgccggcg cgcttcgagc ggacaacatc gagcgcgctc
      961 ggcaattgct cgcggcggcg cgagccgctc cccagcgccc atgcgccgag gccgacagcc
     1021 acgccgaaga cgtctcgcct gcgcggcgct gcccctgctg cggcggccgg atgatcatcg
     1081 ttgagacgtt cgaagggtcg cgcccttggc gttccccgtc gccgagccgg atcaggatcg
     1141 acagctcatg accgtcgccc cgcatcccgc cgcgcaccgt cgatcgcctt cgcctccggc
     1201 cgcgcgccgg aacacgagcg cgacgcccgc gcgaggtcgc cagtcctcct gcaaccgagg
     1261 ccaacgcctc gctcccgcgc atcgtcgcta tagaaaaccc caaccatccg cgcttccgct
     1321 gaaaacaccg agcctcgccg attgccgacg cctcgctcgt cacccctccg cgatcctcaa
     1381 atccccatag gcgcgctcgc caaacggtcc ccctacctcc ccgcggtttc ctccttggga
     1441 ggctttcgag cgccggcccc ggcgcacgcc caaccctcgc aatgggccgg cgcccgaaac
     1501 ccttcaccga tgcggcccgc cagtgtgtgc cttcaacgcc gctctaacgc cctgattgtg
     1561 tttactttgg gcacggcggc aggccgggcc ttccacac
//
LOCUS       DASYNS010000164         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2220674, whole genome shotgun sequence.
ACCESSION   DASYNS010000164 DASYNS010000000
VERSION     DASYNS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2220674"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1764)
                     /locus_tag="VGP50_05185"
     CDS             complement(<1..1764)
                     /locus_tag="VGP50_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8026799.1"
                     /translation="MAPKKMRTPKKAAAKKTRARSRAAPAPEPAAVTPPALPDPTDQQ
                     FRLLVQGVIDYAIFMLDVEGRISTWNAGAERIKGYAPRDVIGRHFSMFYTEEERSAGV
                     PQTLIERATRDGRVETEGWRVRKDGSRFWGFVVIDAIRDDHGRLLGFAKVTRDITERL
                     LAAETLRQSEERFRLLVQGVTDYAIYMLDPEGHVTNWNAGGRRIKGYAPDEVIGRHFS
                     MFYTPEDLQSGLPRRALETAAREGRFEGEGWRLRKDGSRFFANVVIDAIHDAEGKLLG
                     FAKVTRDITERKKAEQRLEQTRSAFVRAQKMEALGQLTGGVAHDFNNLLTVIVSNIEL
                     ASAPERDAQSRARLLDGARRAAARGATLTQQLLAFARRQPLSPKRQRIHALIGAFEAV
                     LRRGCGENCTMAFELAAEPVVAEIDDGQFEAVLLNLVMNARDAMPAGGTVRIRTDLVT
                     VDAARAEAMEGLAPGRYIALSVEDDGTGMPPHVLSHVFEPFFTTKEPGRGSGLGLSQV
                     FGFAKQSGGHVEIESALGLGTTVTLYLPAVAAGAEEETAPRPAAAAAAMRRARRGTAL
                     IVEDDPDVLESAIVSLESLGYD"
     gene            complement(1972..3225)
                     /locus_tag="VGP50_05190"
     CDS             complement(1972..3225)
                     /locus_tag="VGP50_05190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV8026800.1"
                     /translation="MIVGAGAAGLIAARELGRAGKRVTILEARDRCGGRIHPLPVEEF
                     GYAAEGGAEFVHGEAPVTRALLREAGLSLSPIAGARWSARDGVFSQNDAPAPDEGRFY
                     RALAELDADLPIAEFLDRHFAGSHDEELRRSITRMVEGYDAADPARASTLALRDEWMG
                     RGRGRQGRIAGGYGGLIEFLARQCGQQGGSIHLGTAVSGIEESGRGIIAHSGDGRRYE
                     GDAAILTVPLPILEEIVLPPAAREKAAASAGIGYGNVVKFLLRFTSPWWAHAGGRDLA
                     DLSFLFSTAIVPTWWTQHTAKHPVLTGWFAGPKTEGMARLSEAQLVEIGLASLAETFH
                     RAPDRLRKDLVAARAINWAKDPFARGAYSYATPETRKAQSVLGNAEGGPLFFSGEALY
                     RGRDMGTVEAALASGLETARAILAE"
BASE COUNT          498 a         1141 c         1078 g          518 t
ORIGIN      
        1 gtcgtagccg agactctcca gcgacacgat cgccgattcg agcacgtccg ggtcgtcctc
       61 gacgatgagc gcggtgccgc ggcgcgcgcg ccgcatggcc gccgccgctg ccgccggtcg
      121 cggcgcggtt tcttcttcgg cgcccgccgc aacggccggt agatagaggg tcaccgtcgt
      181 gccgaggcca agcgcgctct cgatctccac atggccgccc gactgcttgg caaagccgaa
      241 gacctggctc aggccaagcc cgctccccct ccccggctcc ttggtcgtga aaaacggctc
      301 gaagacatgc gacagcacat ggggcggcat tccggttccg tcgtcctcga cggacagcgc
      361 gatgtagcgt cccggggcca gcccttccat ggcttcggcg cgtgccgcgt cgaccgtgac
      421 gaggtcggtg cggatcctga ccgtgccgcc ggccggcatg gcgtcgcgcg cgttcatgac
      481 gaggttgagc agcactgctt cgaactgccc atcgtcgatc tccgcgacga ccggctccgc
      541 cgcgagctcg aacgccatcg tgcaattctc gccgcaaccg cggcggagga cggcctcgaa
      601 ggcgccgatg agcgcgtgga tccgctgccg cttcgggctg agcggctgtc gccgcgcgaa
      661 agcgaggagc tgctgcgtca gcgtggcgcc gcgtgctgcc gcgcgacgcg cgccgtcgag
      721 gagtcgggcg cggctctgcg cgtcgcgttc gggcgcgctc gcgagctcga tattgctcac
      781 gatgaccgtg aggagattgt tgaagtcatg ggccacgccg ccggtgagct ggcccagcgc
      841 ctccatcttc tgtgcccgga cgaaggccga gcgcgtctgc tcgaggcgtt gctcggcctt
      901 tttccgttcc gtgatgtcgc gcgtgacctt ggcgaagccg aggagcttgc cctcggcgtc
      961 gtggatcgca tcgatgacga cattcgcgaa aaagcgcgag ccgtccttgc gcaggcgcca
     1021 gccctcgccc tcgaatcgcc cctcgcgggc cgccgtctcg agcgcgcgcc gcggcaaccc
     1081 cgactgcaga tcctccggcg tgtagaacat ggagaaatgc cggccgatca cttcgtcggg
     1141 ggcatagccc ttgatgcgcc gcccgcccgc attccagttg gtgacgtggc cttcggggtc
     1201 gagcatgtag atggcgtaat cggtgacgcc ctgcacgagg aggcggaacc gctcctcgct
     1261 ctgccggagc gtctcggcgg ccagcagccg ctcggtgatg tcgcgcgtga ccttggcgaa
     1321 gccgaggaga cggccgtggt catcgcggat cgcgtcgatc acgacgaagc cccagaagcg
     1381 gctgccgtcc ttgcggacgc gccatccttc cgtctcgacc cggccgtcgc gcgtcgcgcg
     1441 ctcgatcagc gtctgcggca ccccggcgct ccgctcttcc tccgtgtaga acatcgagaa
     1501 atgccgcccg atcacgtctc ggggagcgta gcccttgatg cgctccgcgc ccgcattcca
     1561 ggtgctgatg cggccctcga catcgagcat gaagatggcg tagtcgatca cgccctggac
     1621 cagcaggcgg aattgctggt cggtcgggtc ggggagcgcc ggcggcgtga cggcggcagg
     1681 ctccggcgca ggcgccgccc gtgaccgagc ccgcgtcttc ttggcggcag ccttcttggg
     1741 cgttcgcatc ttcttcggcg ccattcagta ttcgcccttg tcacgacggg cactgggaac
     1801 cgtagctaac gctgccgccg gacgaatcgc ccgagaggaa agatcatcgc aaccgccggt
     1861 cgccaccgcg gtcaaccagc acaatctccg cgcgggacgg ggcctcctga gcttgccgaa
     1921 gggcgacgcc gcgggagggt caggggcgcc gaacggcaga gacgatgaga gtcactccgc
     1981 caagatggcc cgcgccgtct ccaatccgct ggcgagcgcc gcctcgacag tgcccatgtc
     2041 gcggccgcga tagagcgcct cgccggaaaa gaaaagaggt ccgccctccg cgtttccgag
     2101 caccgactgc gcctttctcg tctcgggcgt ggcataggaa taggcgcccc gcgcgaacgg
     2161 atcttttgcc cagttgatgg cgcgtgccgc gacgaggtct ttcctcagcc ggtccggcgc
     2221 tcgatggaaa gtctcggcga gcgacgcgag cccgatctcg acaagctggg cctcggaaag
     2281 ccgtgccatc ccctccgtct tgggtcccgc gaaccagccc gtgagcaccg gatgcttcgc
     2341 ggtgtgttgc gtccaccaag tgggaacgat cgcggtggaa aacaggaagg aaagatcggc
     2401 gagatccctg ccgccggcat gggcccacca tgggctggtg aatcgaagca gaaacttcac
     2461 gacattcccg tagccaatgc cggcagaggc ggccgctttc tcgcgtgccg ccggcggaag
     2521 cacgatctcc tccaggatgg gaagcggcac ggtgaggatc gcggcatcgc cctcgtagcg
     2581 ccggccgtca ccgctatggg cgatgatccc tcgaccgctc tcctcgattc cgctcacggc
     2641 cgtgccgaga tggatcgacc cgccttgctg cccgcattgg cgcgcgagaa attcgatcag
     2701 gccgccatag ccgccggcga tccgcccctg ccgaccgcgg ccgcggccca tccattcgtc
     2761 gcggagcgcg agcgtgctgg cgcgcgccgg atcggccgcg tcatagccct cgaccatcct
     2821 cgtgatggaa cgccgcagct cttcgtcgtg acttccggca aaatgccggt cgaggaattc
     2881 ggcgatgggc agatcggcgt cgagttccgc caacgcccgg tagaaccgac cttcatcagg
     2941 agccggagcg tcgttctgcg agaacacgcc gtcccgcgcg ctccatcgcg cgcccgcgat
     3001 gggcgacaat gaaagccccg cctcgcgcaa tagcgcgcgt gtcacgggcg cctcgccatg
     3061 gacgaactcg gcgccgccct ctgccgcata gccgaattcc tcgacgggca gcggatggat
     3121 gcgcccgccg caccggtctc gcgcttcgag gatcgtcacg cgcttgcccg cgcgcccgag
     3181 ctcgcgggca gcgatcaaac ccgcggcgcc tgcgccgacg atcacgatat ggcct
//
LOCUS       DASYNS010000165         7585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1283687, whole genome shotgun sequence.
ACCESSION   DASYNS010000165 DASYNS010000000
VERSION     DASYNS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283687"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VGP50_05195"
     CDS             complement(<1..360)
                     /locus_tag="VGP50_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV8026801.1"
                     /translation="MPYEPPKHCGTCGASLSEVAEAPGQRRCSDDSCGRITYLSPIPV
                     VAAIVEHEGDVVLVRGQGWPETWFGLVTGFLEPKESPEEAARREVKEELGLDAARSAL
                     IGAYAFAQMNQVIIAYHV"
     gene            complement(425..1282)
                     /locus_tag="VGP50_05200"
     CDS             complement(425..1282)
                     /locus_tag="VGP50_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEV8026802.1"
                     /translation="MREQATNHFFRDRAAAEDFLRTLAAAGDGRLPLAEAALALAARD
                     RPLVDLERYHRHLTVLAGDVGEEAAGAGDLAQRAAALSRVILGGYGYQGDTLDYDDLQ
                     NANLIRVIDRRKGLPVALGILYIHAGRAQGWTMTGLAFPGHFLIRIEDEAGRLILDPF
                     HGGKPCAAAELRGLLKATLGDETELAPAHYATVSDRDILLRLQNNLKLRLIESRQTES
                     ALAVIEGMMLFAPDHAALWRDAALLHAECDNLRAAIAALEHFLSLAGAGPERHQAAAF
                     LQKLRARMN"
     gene            complement(1279..2004)
                     /locus_tag="VGP50_05205"
     CDS             complement(1279..2004)
                     /locus_tag="VGP50_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEV8026803.1"
                     /translation="MSPSSPPRLRALLLAATVATASASLSGCILVAAGAGAASGYSVL
                     GQERAPGQQVDDTVIRTLVSQSWDQFNPDLAHDLDATVYQGRVLITGRVPSEAWRQEA
                     VQRSWKVDGVKEVYDEVEVGPETHFSDDARDTVISTRLRNDLLFDGSVKSINYTVKTE
                     NGVVYIIGSARSQAELDSVTSYARNIANVRRVVSYVKIRTGDAGSAQAGGNATPSAST
                     SPSAATPADASTPRAQIEVTPLK"
     gene            complement(2168..2536)
                     /locus_tag="VGP50_05210"
     CDS             complement(2168..2536)
                     /locus_tag="VGP50_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEV8026804.1"
                     /translation="MTRDARRRAFRRGLSAELLCVWHLRLRGYRILARRFRVKSGEID
                     LIARRGAVLAAVEVKARSDLATAGESLTPRQRRRIARALEHFLVQRPDLAGLALRFDV
                     MLVSPRRWPRHLVDAWRVDF"
     gene            complement(2533..3354)
                     /gene="rsmI"
                     /locus_tag="VGP50_05215"
     CDS             complement(2533..3354)
                     /gene="rsmI"
                     /locus_tag="VGP50_05215"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV8026805.1"
                     /translation="MATPIGNMEDVTLRALALLRGADVVACEDTRVTAKLLARHGISC
                     ALVAYHDHNAERVRPLLLERLRGGQTVALVSDAGTPLVSDPGFKLVREAIALGLPVTT
                     LPGASAPLAALVLSGLPSDRFLFGGFLPAKAVARRRALAELAAVPATLIFFEGAPRLA
                     AALGDMAETLGDRPAAVARELTKLHEEVRRGALAELAAHYGTAGPPRGEVVVVVAPPA
                     KDVASPSADALDTQLMAALDAMSIRDASAAVASATGLPRRDVYARALALRGEEGK"
     gene            3585..4754
                     /locus_tag="VGP50_05220"
     CDS             3585..4754
                     /locus_tag="VGP50_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEV8026806.1"
                     /translation="MIGAPLEPTQLPAPPPPPPGNEKLRVALLLPLSGQNAVLGSAML
                     DAAQMALYDVAGERVELLPRDTQGRPDAAADAARGAVADGARLIIGPLVAGEVSAVKP
                     IARAANIPVLAFSTATQLAGDGTYLMGFLSRQEVERVTAFAHGKGVDRFAVLAPRTPY
                     GEIAVDALRGATQAGGASLTHTEYYDPAAQDVTPAVQHLVGVKVDPSTARKLEKLALS
                     TGKPPPDPPPPLDFDAVLLPEGGQRLKAVAPLLPYYDVDPEKVHFLGTGLWDEPGLGS
                     EPALSGGWYAAPPPASRADFERRFAEIYRHAPPRLATLAYDATALAAVLARSATGPDF
                     SAAALTNPSGFAGVDGIFRLRPDGLVQRGLAVLEVHRNGNVVVDPAPTTFQQLGY"
     gene            complement(4824..5813)
                     /locus_tag="VGP50_05225"
     CDS             complement(4824..5813)
                     /locus_tag="VGP50_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026807.1"
                     /translation="MPKLGLLETRRGTLLLLTLAVVSSVLLTFPIWRVLFFGDIPRAV
                     PITATIVSFIVGLPLILYMQQVIHHLADSERALTRLTEQLAGAREKAELASEAKSEFV
                     ARMSHELRTPLNAVIGYSEMLLEDAEADGFGKHTDDLRRIAGAGKHLLALVNDVLDLS
                     RIEAGRMEVSVAPIDLGGFIDEIAATCKTLIASNGNTLTIERDETGIVLGDATKLRQV
                     VLNLLSNAAKFTERGEITLSVRRQRQGAGDWLAIAVRDTGIGIAPKVLMKLFDSFTQA
                     EPMITRKYGGSGLGLALSRKLCRLMGGDIVAESEPGYGSCFTVRIPASMAAMI"
     gene            6141..7418
                     /locus_tag="VGP50_05230"
     CDS             6141..7418
                     /locus_tag="VGP50_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026808.1"
                     /translation="MEFAAEVFDQVQRLFDLNGFNDHQLHCVLRFAARPDAAVLKKAV
                     IASIEAIPILGTRYVANDQPHWESIDPGGFDRAFATAGTEAEFEEFLASSVDEGEGPQ
                     VSVRLLDTRSAFAVAIKLNHMICDAAGFKEYLYFLCRIYAGLTVDSAFRPVAIDGDRS
                     IRGVLKRFGPGVKLKSLLQRDKDNNRAGGERFPLDEYGAVRPFILTRKLVRERVAALR
                     DYCRANGATLNDAVLTAFYRCVFRRLKPRPGAELWIPIMVDMRRHLGEAERCASLTNL
                     SSMVGTQIAYLPDEGFADTLGRVKAIMDENKVGGIGLNAFVKLDLTCRLLGDKSANRI
                     LRTKLKNPLICMTNIGVLDADRLALGEARPYDAFMCGSVKYKPYFQLAMSSFAGEITL
                     SVNEYGSAADRESILAFLDEVEAELIPPIRSSV"
BASE COUNT         1228 a         2529 c         2568 g         1260 t
ORIGIN      
        1 gacgtgatag gcgatgatca cctggttcat ctgcgcgaag gcataggcgc cgatgagcgc
       61 gctccgggcc gcgtcgagcc cgagctcttc cttcacctcg cgccgcgccg cttcctcggg
      121 gctctctttc ggctcgagga agccggtgac gaggccgaac caggtctcgg gccagccttg
      181 gccccgcacc agaaccacat cgccctcatg ctcgacgatg gcggcgacca cgggtatcgg
      241 cgacagataa gtaatacggc cgcagctgtc atcgctgcag cggcgctgcc cgggcgcctc
      301 cgcgacctcg ctcagcgagg cgccgcaggt cccgcaatgc ttgggcggct cataaggcat
      361 ccggcgtgac ctccctgccc tcagcgttcg ctaatgacaa agcctcgctc cttgacgaga
      421 tgagctaatt cattcgcgcc ctgagctttt gcaggaacgc tgcggcctgg tggcgctcgg
      481 gaccggcgcc ggcgagcgac aggaagtgtt cgagcgcggc aatggcggcg cgcaggttgt
      541 cgcattccgc atggaggagc gctgcgtcgc gccacagcgc cgcatggtcg ggcgcgaaca
      601 gcatcatgcc ttcgatcacg gcgagcgcgc tctcggtctg gcggctctcg atcaggcgga
      661 gcttcaggtt gttctgcagc cgcagcagga tgtcgcggtc gctgacggtc gcgtaatgcg
      721 cgggcgcgag ctccgtctcg tcgccgagcg tcgccttgag gagaccgcgc agctcggcgg
      781 cggcgcaggg cttgccgccg tggaacggat cgaggatgag gcgccccgcc tcgtcctcga
      841 tccggatgag aaaatgcccg gggaaggcaa ggccggtcat cgtccagccc tgcgcgcggc
      901 cggcatggat gtagagaatg ccgagcgcca ccggcagccc tttgcggcgg tcgatgacgc
      961 ggatgaggtt ggcgttctgc agatcgtcgt agtcgagcgt gtcgccctga taaccgtagc
     1021 cgccgaggat cacgcggctg agcgcggcgg cgcgttgagc gagatcgccg gcgcctgccg
     1081 cctcctcgcc gacgtcgccg gcaagcacgg tgagatgccg gtgatagcgt tcgaggtcga
     1141 caagcggccg gtcgcgggcg gcgagcgcca gcgccgcttc ggccaacggc aaacgtccgt
     1201 cgcccgccgc agcgagcgtg cgaagaaagt cttcggccgc cgcgcgatcg cggaagaagt
     1261 ggttcgtcgc ttgctccctc acttcagtgg cgtcacctcg atctgcgcgc ggggtgtgct
     1321 cgcgtctgcg ggagtggcgg ccgacgggct cgtgctggca gagggcgtgg cgttgccgcc
     1381 cgcctgcgcc gatccggcat cgcccgtgcg gatctttaca taggaaacga cgcggcggac
     1441 attggcgatg ttgcgcgcat agctggtgac ggagtcgagc tccgcctggc tccgcgccga
     1501 gccgatgatg tagacgacgc cgttctcggt cttgacggtg tagttgatcg atttgacgct
     1561 gccgtcgaac agcagatcgt tcctgagccg ggtggagatg acggtgtcgc gcgcgtcgtc
     1621 gctgaaatgg gtctcgggcc cgacctcgac ctcgtcatag acctccttga cgccgtccac
     1681 tttccaactg cgctgcacgg cctcctggcg ccacgcctcg ctcggcacac gtcccgtgat
     1741 cagcacgcgg ccttgataga cggtggcgtc gagatcatgc gccaggtcgg gattgaactg
     1801 gtcccaggac tggctcacca gcgtccggat gacggtatcg tcgacctgct ggccgggcgc
     1861 gcgctcctgg cccagcacgg agtagccact ggcggcaccc gcgcctgcgg ccacgagaat
     1921 gcagccggag agcgaagccg atgccgtggc gaccgtggcg gcgagcagga gagcacgaag
     1981 gcggggcggc gaagacggag acacggcgag actctcccag gcaacgcaaa gggaccccta
     2041 ttctcccgca gactcgcggc gacgcaaggg tcgcattgtg gcgagaccaa ggcagccccg
     2101 gaaatgatca gctatcagtt gttggttatc agtcgtactt acaaccgaaa actgaaaacc
     2161 gaccacttta aaaatcgacg cgccaggcgt cgacgagatg ccgcggccag cggcggggcg
     2221 agaccagcat cacgtcgaag cgaagcgcga ggccggcgag gtcggggcgc tgcacgagga
     2281 aatgctcgag cgcgcgggcg atgcggcggc gctggcgcgg cgtgagcgac tcgccggcgg
     2341 tcgcgagatc gctccgcgcc ttcacttcga ccgcggcgag cacggcgccg cgccgcgcga
     2401 tcaggtcgat ctcgcccgac ttcacgcgaa agcgccgcgc cagaatgcga tagccccgga
     2461 gccgcagatg ccagacgcaa agcagctcgg ccgagaggcc gcggcgaaat gcccgccgcc
     2521 gcgcgtccct cgtcatttgc cctcctcgcc ccggagcgcc agcgcccgcg catagacgtc
     2581 gcgccgcggc agccctgtgg cggaagcgac cgccgcgctc gcgtcgcgga tgctcatggc
     2641 gtcgagcgcc gccatgagct gcgtatcgag cgcatccgcg gagggtgaag cgacgtcctt
     2701 tgccggcggt gccacgacga cgacgacctc gccgcgcggc ggtcctgccg tcccataatg
     2761 cgcggcgagc tccgcgagcg cgccgcgccg cacttcctca tggagcttgg tgagctcgcg
     2821 cgcgaccgca gccggacggt cgccgagcgt ctccgccatg tcgccgagcg cggccgcgag
     2881 ccgcggcgcc ccctcgaaga agatgagtgt cgccgggacg gccgccagtt ccgcgagagc
     2941 gcggcgccgc gccaccgctt tcgccggcag gaagccgccg aagaggaagc ggtcgctcgg
     3001 caggcccgac agcaccagcg cggcgagcgg tgccgatgcg ccgggcagcg tcgtcaccgg
     3061 cagtcccagg gcgatcgcct cgcggaccag cttgaacccc gggtcggaga cgagcggcgt
     3121 ccctgcatcg gacaccagcg ccacggtttg gccgccgcgc aggcgctcga ggagcagcgg
     3181 ccgcacgcgc tcggcattgt ggtcgtgata ggcgacgaga gcgcacgaga tgccgtggcg
     3241 cgccagcagc ttcgccgtga cgcgcgtgtc ctcgcaggcg acgacgtcgg cgccgcgcaa
     3301 cagcgcaagc gcgcgcaagg tcacgtcctc catgttgccg atcggcgtcg ccacgacgta
     3361 gagtccgggc gccgggcgtt tacttccgtc gactcggtgg ttaggctcgc cgccgcgttc
     3421 tctgggaccg acggaggttg cgcctgtttc tcccgcttta tccgcgcctc gcctgcccgc
     3481 gcgccgttgc cgcgccatgg ctggtcctct ccgcgctcgc gctgtccgct tgccaggagc
     3541 cgaccatatc gagagtcgcg ccgccgctgc aaggagcgcc gccggtgatc ggagcgccgc
     3601 tcgagccgac gcagctgccc gcgccgccgc cgccaccgcc cggcaatgag aagctgcgcg
     3661 ttgccctgct cttgccgctc tcgggccaga acgccgtgct cggcagcgcc atgctcgatg
     3721 cggcgcaaat ggcgctttac gacgtcgccg gtgagcgggt cgagctcttg ccgcgcgaca
     3781 cccaagggcg gccggacgcg gccgccgacg ccgcgcgggg cgccgtcgcc gacggcgcgc
     3841 gcctcatcat cgggccgctg gtggccggcg aagtcagcgc ggtgaagccg atcgcgcgcg
     3901 cggcgaacat acccgtgctc gctttctcga cggcgacgca gcttgccggc gacggcacgt
     3961 acctcatggg ctttctctcg cgccaagagg tcgagcgcgt cacggctttc gcgcatggca
     4021 aaggcgtcga ccgcttcgcg gtgctggcgc cgcgcacgcc ttatggcgag atcgccgtcg
     4081 acgcgctgcg cggggcgacg caggcgggcg gcgcgagcct gacgcacacc gagtattacg
     4141 atccggcggc gcaggatgtg acaccggccg tacagcatct cgtcggcgtc aaggtcgacc
     4201 cgtccacggc gcggaaactc gagaagctcg ccttgagcac aggcaagccg ccgcccgatc
     4261 cgccgccgcc gctcgatttc gatgcggtgc tgcttcccga aggcggccag cgcctcaaag
     4321 cggtggcgcc gctccttccc tattacgatg tcgatcccga gaaggtgcat ttcctcggca
     4381 ccggcctctg ggacgagccg ggcctcggca gcgagcccgc cttgtcgggc ggctggtatg
     4441 cggcaccgcc gccggcgtcg cgcgccgatt tcgagcggcg cttcgccgag atctatcgcc
     4501 atgcgccgcc gcgcctcgcg acgctcgctt acgatgcgac ggctcttgcc gccgtgctcg
     4561 cgcggagcgc caccggtccc gatttctcgg cggcggcgct caccaatccc agcggctttg
     4621 ccggcgtcga cggcatcttc cgcctgcgcc ccgacggcct cgtgcagcgc ggcctcgccg
     4681 tgctcgaagt gcaccgcaac ggcaacgtcg tggtcgaccc ggccccgacg acattccagc
     4741 agctcgggta ttgaggaagt tgtcagttgt cagttatcag tcgtcagttc gactggagac
     4801 tgacaactga agactgaaaa cttctaaatc atcgccgcca tcgatgccgg gatgcgcacg
     4861 gtgaagcagg agccgtagcc gggctcgctc tcggcgacga tgtcgccgcc catgaggcgg
     4921 cacagcttgc ggctcaaggc gaggcccaat ccgctgccgc catatttgcg ggtgatcatc
     4981 ggctctgcct gcgtgaagct gtcgaagagc ttcatcagca ccttgggcgc gatgccgatc
     5041 ccggtgtcgc gcacggcgat cgccagccag tcgccggcgc cctggcgctg ccgccggacg
     5101 ctcaacgtga tctcgccgcg ttcggtgaat ttcgcggcgt tgctcaggag attgagcacc
     5161 acctggcgca acttggtcgc gtccccgagc acgatgccgg tctcgtcgcg ctcgatggtg
     5221 agcgtgttgc cgttgctggc gatgagcgtc ttgcaggtcg cggcgatctc gtcgatgaag
     5281 ccgccgaggt cgatgggcgc caccgagacc tccatccgcc ccgcctcgat ccgcgagagg
     5341 tcgaggacgt cgttcaccag cgccaggaga tgcttgcccg cgccggcgat gcggcggaga
     5401 tcgtcggtgt gcttgccgaa gccgtcggcc tcggcgtctt cgagcagcat ctcgctatag
     5461 ccgatcaccg cgttcaaggg cgtgcgcagc tcatggctca tgcgcgcgac gaattcggat
     5521 ttcgcctcgc tcgcgagctc cgccttctcg cgcgcgccgg ccagctgctc ggtgagacgg
     5581 gtcagagcgc gctcggaatc cgcgaggtga tgaatcacct gctgcatgta gagaatgagc
     5641 ggcaggccga cgatgaagct gacgatggtc gccgtgatcg gcacggcgcg cgggatgtcg
     5701 ccgaagaaga ggacgcgcca gatcggaaag gtcagcagga cggaggagac gacggcgagc
     5761 gtcagcagca acagcgtgcc gcgccgcgtc tcgagcaggc cgagtttcgg catggccagc
     5821 ccggcatcgc cgatcagccc aaacctgccg gctgttggat tttctgcgga catgctttgc
     5881 ggccagggcc tttcttcgag gcagtaccca cgtttaaccg cgatcgatga aaagccgctt
     5941 actttttccg acaattttag cgattggccg acccagcaat ccgatagcga acaaagttgg
     6001 cccaagggaa tttgctaccg gaacgcgaat ggttatgtcc ttcgtcgtgg gcggcgttac
     6061 cgcgtcgcgg agcgcattgc atccgtcggc tactcatctg tccgatcgcg ctatagtgcg
     6121 cgcgctagga gagtcggccc atggaattcg ccgccgaggt tttcgaccag gtccaacgtt
     6181 tgttcgacct caacggcttc aacgaccacc agcttcattg cgtcctgcgc ttcgccgctc
     6241 gacccgacgc cgcagtcctg aagaaggcgg taatcgcctc gatcgaggcg attccgatcc
     6301 tgggtacgcg ctatgtcgcg aacgaccagc cgcactggga gagcatcgat cccggtggtt
     6361 tcgaccgcgc cttcgccact gcagggaccg aggcggagtt cgaggaattc ctggcgtcgt
     6421 cggtcgatga aggagagggc ccccaggtga gcgtccgcct cctcgacaca cggtccgcct
     6481 tcgcggtcgc gatcaagctg aaccacatga tttgcgatgc cgcgggcttc aaggaatatc
     6541 tctatttcct ctgccggatt tacgccgggc tgacggtcga ttcggcgttt cggcccgtcg
     6601 cgattgacgg cgaccggagc attcgcggcg tgctcaaacg cttcggcccc ggcgtcaagc
     6661 tgaagtccct gctgcagcga gataaggaca acaaccgggc cggcggtgag cgatttccgc
     6721 tcgacgagta tggcgcggtg cgccccttta ttctcacgcg caagctggtg cgcgagcggg
     6781 tcgcggcgct ccgggattat tgccgagcga acggagcgac gctgaacgat gcggtgctga
     6841 ccgcctttta ccgctgcgtg tttcggcgcc tcaagccgcg tcccggcgcc gagctttgga
     6901 ttcccatcat ggtcgatatg cgccgccatc tcggcgaagc cgagcggtgc gcctcgctga
     6961 ccaatctctc ctcgatggtg ggaacgcaga tcgcctatct tcccgacgag ggcttcgcgg
     7021 atactctcgg cagggtcaag gcgatcatgg acgagaacaa ggtcggcggg atcggcctca
     7081 atgctttcgt caaactcgat ctgacgtgtc ggctactcgg ggacaagagt gccaaccgca
     7141 tactgcgaac gaagctgaag aacccgctca tctgcatgac caatatcggc gtcctcgatg
     7201 ccgatcgcct ggcccttggc gaggcgcgcc cgtatgacgc cttcatgtgc gggtcggtca
     7261 agtacaagcc ctatttccag ctcgccatga gcagcttcgc gggcgagatc accctcagcg
     7321 tcaacgagta cggaagcgct gccgaccgcg aaagcatcct cgccttcctc gacgaggtcg
     7381 aggcggaact gattccacca atccgatcga gcgtttgaag acgagcgact tttgcgttgg
     7441 ctaactagtc ttactgagtc agaaagggtg agtcagaacg ccgcagggtg agtgtaccaa
     7501 ggctctggta ggtttgagag attcattttg gcgggggagc tgaggtgggt cttcgaagaa
     7561 gggacgagag tgaggctcgg tttgc
//
LOCUS       DASYNS010000166         5090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1283912, whole genome shotgun sequence.
ACCESSION   DASYNS010000166 DASYNS010000000
VERSION     DASYNS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283912"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="VGP50_05235"
     CDS             <1..1125
                     /locus_tag="VGP50_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026809.1"
                     /translation="NTLIEVQRETLDNLYEAIAVFGSDGRLKLSNPSYAQVWQLSPVD
                     LVGEPHVSEIIEKQRAFFDDGGDWTELKRRIIARLTAHLAENERLARRDGSILQAATV
                     PLPDGNILLSYLDVTDSTRVENALRERNEALETAGRLKSEFIANVSYELRTPLNAIIG
                     FAEILTNQYFGELTPRQLDYSRGILDSSHRLLSLINDILDLATIEAGYMTLETTDLDI
                     HTLMSSVLSLTRERARKQNLKLDFDCPTDIGSLVGDERRLKQALFNLISNAIKFTPAG
                     GTVRLAARRAGDRVSLIVADTGIGVPREDQARVFEKFERGNPSARQSGPGLGLSLVKS
                     FIELHGGTVEMDSRPGVGTTVTCYLNAAPIEAAARRSAGE"
     gene            complement(1168..1542)
                     /locus_tag="VGP50_05240"
     CDS             complement(1168..1542)
                     /locus_tag="VGP50_05240"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEV8026810.1"
                     /translation="MKFLLWSVALAFIQMLVAVSGATLKVGLPRLAGNREGLPELTGW
                     AGRAKRAHWNMLESLPLFIALVLIAEIARRTNATTLLGAELFFWGRVAHGIIYVAGLP
                     WLRTLAWAVSVIGLILIFLQLI"
     gene            complement(1624..2646)
                     /locus_tag="VGP50_05245"
     CDS             complement(1624..2646)
                     /locus_tag="VGP50_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV8026811.1"
                     /translation="MAGESHRTDVAIIGAGPVGLFAVFECGMLRMGCHVVDALDMLGG
                     QCVALYPEKPIYDIPGHPVVGAADLVEKLADQAAPFHPVYHLAQQVERVAPLPAGGFR
                     LETSLGTAIEARAVIIAAGVGAFGPNRPPLAGIESFEGKSVFYLVKRREDFRGKRIVI
                     AGGGDSAVDWALSLSEVAAAVLVVHRRPKFRAAPESAARMERLAAAGRIELVIPYQLA
                     ALEGRDGRLEAVVVADLEGRTRRLSADVLLPFFGLSMSLGPIAEWGLDLERGHIAVDP
                     ATAATSVPGVFAIGDIATYPGKLKLILSGFAEAALAAHAMFPLVHPGEALHFEYSTTK
                     GVPGRP"
     gene            2755..3234
                     /gene="tsaE"
                     /locus_tag="VGP50_05250"
     CDS             2755..3234
                     /gene="tsaE"
                     /locus_tag="VGP50_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV8026812.1"
                     /translation="MSAATAFDIDLPDETATAGFAQRLARRARRGDIIGLMGDLGSGK
                     TTFARAFIRALGSGHEEVPSPTFTLVEVYGFPGHPPVWHFDLYRLTAPEQAYELGIED
                     AWSDGISLVEWPERLGPLLPAEHLLLRLVAAQQAQARVAKLTASSDWAPRLEGVGRG"
     gene            3227..4237
                     /locus_tag="VGP50_05255"
     CDS             3227..4237
                     /locus_tag="VGP50_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV8026813.1"
                     /translation="MAERAAQIAAFLAAAGWAEVPRRPLAGDASFRRYERLETAGRRA
                     VLMDAPPPKEDVRPFIAVARLLHGQGFSAPQVLAEDVAAGLLLLEDFGDSTYTRLLAA
                     GADEKRLYCLAVDLLIALHRGVKLPGASAVPPYSDERLLSEAALLVDWYLPAITGQPT
                     EPALREEYLACWRGLLPVARSVPDTLVLRDYHVDNLMLLDGRSGVAACGLLDFQDAVI
                     GPCSYDLVSLLEDARRDVPADLVAAMVEQYLAAFPELDRAAFAASYAVMGAQRNCKIV
                     GIFTRLCVRDGKPIYLAHIPRVWRLIEQDLKHPALAPMARWLDRHVPPPLRRTPPART
                     AA"
     gene            4234..4953
                     /locus_tag="VGP50_05260"
     CDS             4234..4953
                     /locus_tag="VGP50_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV8026814.1"
                     /translation="MTVVSCGMVLAAGLGTRMRPITDRIPKPLVPVAGKPLIDHAIDR
                     LAEAGVKRVAVNLHYKADMVARHLAARQTPTIALSREDDLLETGGGILKALPLLDDAF
                     YVVNSDVLWLDGKIAALRRLARAWDPERLDAMLLVQRTTSALGYEGPGDFFVDAWGAL
                     RRRREREVAPHLFAGVQILHRRLFDGCTPGKFSLNPLWDRAIAAGRLAAIVHDGEWYH
                     IGTPEGLALAEERLATHRIER"
BASE COUNT          835 a         1743 c         1695 g          817 t
ORIGIN      
        1 aacacgctca tcgaagtgca gcgcgagacg ctcgacaacc tctacgaggc gatcgccgtc
       61 ttcggcagcg acggacgcct gaagctctcc aatccctcct acgcccaggt atggcagctc
      121 tcgccggtgg acctcgtggg cgagccccat gtcagcgaga tcatcgagaa gcagcgcgcc
      181 ttcttcgatg acggcggcga ctggacggag ctcaagcgcc gcatcatcgc gcgattgact
      241 gcgcatctcg ccgagaacga gcgcctggcg cgccgcgacg gctcgatcct gcaggcggcg
      301 acggtaccgc tgcccgacgg caacatcctc ttgagctatc tcgacgtcac cgactcgacc
      361 cgggtcgaga acgcgctacg cgaacgcaac gaggccctcg aaacggccgg ccggctcaag
      421 agcgagttca tcgccaacgt ctcttacgag ctgcgcacgc ccttgaacgc catcatcggt
      481 ttcgccgaga tccttaccaa ccagtatttc ggcgagctca cgccgcgcca gctcgattac
      541 agccgcggca tcctcgacag ttcgcaccgg ctgctctcgc tcatcaacga catcctcgat
      601 ctggcgacga tcgaggcggg ctacatgacg ctcgagacca cggatctcga catccacacg
      661 ctgatgtcga gcgtgctgtc gctgacgcgc gagcgcgcgc gaaagcagaa cctgaagctc
      721 gacttcgatt gcccgaccga catcggctcg ctcgtcggcg atgagcggcg gctcaagcag
      781 gcgctcttca atctcatctc gaacgccatc aagttcacgc cggcgggcgg caccgtgcgc
      841 ttggcagcgc gtcgcgccgg cgatcgcgtc tcgctcatcg tcgccgacac cggcatcggc
      901 gtaccgcgcg aggaccaggc gcgcgtcttc gagaaattcg agcgcggcaa cccgagcgcg
      961 cgccaatcgg gtccggggct cgggctctcg ctcgtcaaga gcttcatcga gctccacggc
     1021 ggcacggtcg agatggattc gcgacccggg gtcggcacca cggttacctg ctatctgaac
     1081 gccgctccga tcgaggccgc ggcgcggcgc tcggcggggg agtgaccaag ccacccgccc
     1141 gcccgcagcc gaacgctgcg cgcccgccta gataagctgc aggaaaatca ggatgaggcc
     1201 gatgacggag acggcccagg ccagcgtgcg cagccagggg aggccggcga catagatgat
     1261 gccatgggcc acgcgccccc agaagaagag ctccgcgccc agcagcgtcg tggcattggt
     1321 ccgtcgggcg atctcggcga tcagcaccag cgcgatgaag agcggcaagc tctccagcat
     1381 gttccagtgc gcgcgtttcg cccgccccgc ccatccggtg agctcgggca agccttcgcg
     1441 gttcccggcg agcctcggca gacccacctt cagcgttgcg cccgacacgg cgacgagcat
     1501 ctggatgaag gcgagcgcca ccgaccagag caaaaatttc atatccggtg tcatgtttcc
     1561 cccaaaggtt gaatttagcg ccgccagaat agccgaaaac ggcgacaagt cagccgtcgc
     1621 gatctacggg cgacccggca cgcccttggt cgtggagtat tcgaaatgca gcgcttcgcc
     1681 gggatgcacg agcgggaaca tggcgtgagc ggccagcgca gcctcggcga agcccgagag
     1741 aatgagcttg agttttcccg gatatgtggc gatatcgccg atggcgaaga cccccggtac
     1801 cgacgtcgcg gccgtcgccg ggtcgactgc gatatggccg cgctcgaggt cgagtcccca
     1861 ttccgcgatc gggccgagac tcatcgagag tccgaaaaag ggcagcagca catcggcgga
     1921 caggcgccgc gtgcggcctt cgaggtcggc gacgacgacc gcttcaagcc ggccgtctcg
     1981 accctcgagc gccgcgagtt gatagggaat gacgagctcg atccggccgg ccgcggcgag
     2041 ccgctccatc cgcgccgccg attcgggcgc ggcacggaat ttggggcggc ggtggacgac
     2101 cagcacggct gcggccacct ccgagagcga tagcgcccaa tcgaccgcgg aatcgccgcc
     2161 gccggcgatg acgatacgct tgccgcggaa gtcctcgcgg cgcttgacga ggtagaagac
     2221 gctcttgccc tcgaagctct cgatgccggc gaggggcgga cggttggggc cgaaggcgcc
     2281 gacgcccgcg gcgatgatca cggcgcgcgc ctcgatggct gtgccgaggg acgtctcgag
     2341 ccggaaaccg ccggccggga gcggcgccac gcgctcgacc tgctgtgcca ggtggtagac
     2401 gggatgaaac ggcgccgcct gatcggcgag cttctccacg agatcggcgg cgccgaccac
     2461 ggggtggccg ggaatgtcgt agatcggctt ctccgggtag agcgccacgc attggccgcc
     2521 cagcatgtcg agggcatcca ccacgtggca gcccatcctg agcatgccgc actcgaaaac
     2581 ggcgaagagg ccgacagggc cggcaccgat gattgccaca tccgtgcgat gactttcgcc
     2641 tgccatcgtc tgtcccgcgg gttgctgcca gtctaccatg gcggcatgac gctgcccgtg
     2701 caaggacgcc gcacccgttg cggtgcgccg cccgaaagga ataaaaggag ccccgtgagc
     2761 gccgccactg ccttcgacat cgatctgccg gacgagactg cgacggccgg cttcgcccag
     2821 cgcctcgcca ggcgggcgcg gcgcggcgac atcatcggac tgatgggcga tctcggtagc
     2881 ggcaagacca cctttgctcg cgctttcatt cgtgctctgg gctccggcca tgaggaagtg
     2941 ccgagcccga ctttcacgct ggtcgaagtc tacggctttc ccggccaccc tccggtctgg
     3001 catttcgatc tctatcgcct gacggcgccg gaacaggcct atgagctcgg catcgaggac
     3061 gcctggagcg acgggatcag cctcgtcgaa tggccggagc ggcttggtcc tctgctgccg
     3121 gccgagcatc tgctgttgcg gcttgttgcg gcacagcagg cgcaggcacg agtcgcgaag
     3181 ctgacggcct cttccgactg ggcgccgcgg ctcgaaggcg tgggccgtgg ctgagcgcgc
     3241 ggcgcagatc gccgccttcc tcgcggccgc cggctgggcg gaggtgccgc gccggccgct
     3301 cgcgggcgac gcatccttcc gccgctatga gcggcttgag acggcggggc gccgcgccgt
     3361 gttgatggat gcgccgccgc ccaaggagga tgtgcgtccc ttcatcgccg tcgcccgact
     3421 gctgcacggc cagggcttca gcgcgccgca ggtccttgcc gaggatgttg ccgcgggcct
     3481 gctgctgctc gaggatttcg gcgacagcac ctacacacgg ctcctcgccg ccggcgccga
     3541 cgaaaagcgg ctctattgcc tggccgtcga tctgctgatc gcgcttcatc gcggcgtcaa
     3601 gcttcccggt gcttctgccg tcccgcccta cagcgacgag cgcctgctca gcgaagcggc
     3661 gctgctcgtc gactggtatc tgccggcgat caccgggcag ccgaccgagc cggcgctgcg
     3721 cgaggaatat ctcgcctgct ggcgcggcct gctgccggtc gcgcgcagcg tgccggacac
     3781 cctcgtgctg cgcgactatc acgtcgacaa tctgatgctg ctcgacggcc gctccggcgt
     3841 tgccgcttgc ggactgctcg acttccagga cgcggtcatc ggaccttgca gctatgatct
     3901 cgtctcgctg ctcgaggatg cgcgccgcga cgtgccggcc gatctcgtcg cggcgatggt
     3961 agagcagtac ctcgcggcat ttcccgagct cgatcgcgcc gctttcgccg cgtcttatgc
     4021 cgtgatgggc gcccagcgca attgcaagat cgtcggcatc ttcacgcgcc tttgcgtgcg
     4081 cgacggcaag cccatctatc tcgcgcatat tccgcgcgtc tggcgtctca tcgagcagga
     4141 tctgaagcat ccggcgctcg cacccatggc gcgctggctc gaccggcatg ttccgccgcc
     4201 gttgcgccgc accccgccag cccggacggc cgcatgaccg tggtcagctg cggcatggtg
     4261 ctggcggcgg gcctcggcac gcgaatgcgg ccgatcaccg accgcatccc gaagccgctc
     4321 gtcccggtcg ccggcaagcc gctcatcgac cacgcgatcg atcgtctcgc cgaggccggg
     4381 gtgaagcgcg tcgcggtcaa tctccactac aaggccgata tggtggcgcg tcacctcgcg
     4441 gcgcggcaaa caccgacgat cgcgctgtcg cgcgaggacg atctgctcga gactggcggc
     4501 ggcatcttga aagcactgcc gctgctcgat gacgctttct acgtcgtcaa cagcgacgtg
     4561 ctgtggctcg acggtaagat cgcggcattg cggcggctgg cgcgcgcctg ggatccggag
     4621 cggctcgacg ccatgctgct ggtgcagcgc acgacgagcg cgctcggcta tgaggggccg
     4681 ggcgatttct tcgtcgatgc ctggggcgcg ctccgccgtc gccgggagcg cgaggtggcg
     4741 ccccatctct tcgccggagt ccagatcctg catcgccgtc tgttcgatgg atgcacgccc
     4801 ggcaagttct cgctgaatcc gctctgggac cgcgctatcg ccgcgggccg cctcgccgcc
     4861 atcgtgcatg acggcgaatg gtaccacatc ggcacgcccg agggcctggc gctcgccgaa
     4921 gagcgcctcg cgacacaccg gatcgagcgc tagtcttact gagtcagaaa gggtgagtca
     4981 gaacgccgca gggtgagtgt accaaggctc tggtaggttt gagagattca ttttggcggg
     5041 ggagctgagg tgggtcttcg aagaagggac gagagtgagg ctcggtttgc
//
LOCUS       DASYNS010000167        11136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_660503, whole genome shotgun sequence.
ACCESSION   DASYNS010000167 DASYNS010000000
VERSION     DASYNS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11136
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660503"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            495..1988
                     /locus_tag="VGP50_05265"
     CDS             495..1988
                     /locus_tag="VGP50_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEV8026815.1"
                     /translation="MANAVPAPLKPAALSLKRAGLAAAFLVLALILLLPAAAGLPAAG
                     QRMLAVFAFAVIVWVTEALDYAVSAAVIAALMAFLLGTAPNAANPKALLGTSAGLATA
                     VSGFGSTALTLVAAALFLSAAMTITGLDRRIALLVLSKVATRTSHVVIGAIVVSTLLA
                     FLVPSSTARAAAVVPIMMGVIAAFRLPKSSRFAGLVMITTVQAVTIWNVGIKTASAQN
                     MVAIGFIQKLLDTDVTWISWLVAAAPFSIVLSVALYFIMMAMMPPETTEIAGGQASVA
                     KALSELGPMTGAETRLLLVSLVLLGFWATEGVLHAYDSATTTTIAVALLFLPGIGVMD
                     WKKASVLIPWGTVLLFGVGIGLGTALLQTQAAPWLANIIVARFGLDDLGPLAILAVMA
                     AFLVVIHLGFASGTALASSMIPIVIAVLQKVQTPGISVVGMTLLLQFVVSFGFILPVN
                     SPQGMVAYGTGTFSVRDYIRTGAVITVVAYLLVLLFSATYWRWLGYV"
     gene            2043..2690
                     /locus_tag="VGP50_05270"
     CDS             2043..2690
                     /locus_tag="VGP50_05270"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV8026816.1"
                     /translation="MTDRPAPSSRQPIFNIPAGTMALLIINIAVHGLRLLLSDSSDDR
                     IVRDFAFIPARYTTGLLGWPALVDPVTYQFLHANLTHLFVNMLALLAFGSGVERRIGR
                     WRMLALAILSGIIAAGAHLAVYPSSDVPVVGASGAISGLFGAVLRMMSRGMAGRRSGL
                     WPLIALWVIVTVIAGQTGMPGEPDAPIAWVAHLGGFAAGLALFGFFDRTAGRRSA"
     gene            2823..3953
                     /locus_tag="VGP50_05275"
     CDS             2823..3953
                     /locus_tag="VGP50_05275"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEV8026817.1"
                     /translation="MTPNSTQHVTLGDPVPGFTARTVTGASVSLHVDAGRWILLSFLG
                     SLADQRAAEELAALLREARLFSEPHLVAYVILTEPPQPEALTMLAAVSGPALAFIADY
                     DGAITRSFGAELMRRSVVLDPMQRAIADIPWDHAAGHAALLCQVLRELPPVADSAGVP
                     LWAPALIVPRVLDFALCDFLVKLYDAMGGEESGFMLDQDGKTASVIDHRLKRRRDLII
                     VDPELRETIRGQILRRLIPAIDQFFGFAATRMDRYIVSCYDSAVGGHFFRHRDNVNAG
                     AQHRRFAVSINLNKDYEGCDLVFPEFGPRPYRPPVGGAVAFSAGMLHEVTPITRGRRH
                     VFVPFLYGEADAARRESNNARLRDGESLYVGGADRLFPEAAE"
     gene            3956..4936
                     /locus_tag="VGP50_05280"
     CDS             3956..4936
                     /locus_tag="VGP50_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026818.1"
                     /translation="MGHDLAALERFTPVALNLDAEPSIDWGDLDGVRFSEPFFDQTIE
                     RWAGGPAPRLVRTGLDALAALDAAPSLDPAALIFHLSRCGSTLVSRLLATHPRTLVIS
                     EPKPLNALLMAGPPEFDAERQAYLLRLLVRALGRRRTGGERHYVLKLSSWNVTRLALF
                     RRAFPDAPLVWLQRAPSEIMASISADPPGWMQLRHVPSQAEALFGIPAASSAGLDAEE
                     FCARALAAMLAAARGAEDAGALLVDYRELPDAVWQRVAPFAGIALDDVATGRMREEAR
                     FHSKDTARRLFTGDPAETRPASDRAHALVADLAEPLYQALDRRRRAAPDV"
     gene            5086..6111
                     /locus_tag="VGP50_05285"
     CDS             5086..6111
                     /locus_tag="VGP50_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEV8026819.1"
                     /translation="MKLFPLLLGRAKVPYGQFYGGLSGWQGLGALLRYVFDKSHFIEV
                     PIHAYLLEHPREGLVLIDAGICPEQAHHHRQYYRGLLGMTLNDDEYRQAANETLQEQL
                     KPLGHSVNDIRTVILTHLHDDHAGGLRQLPKAKVVIAKTEWEARHMKLLGFIPMFYEP
                     SFGAVAQWQYVSFESSAFHSFDQSEDLFRDGSVRLLPTPGHTPGHTSALIDMGEYRLL
                     VTGDCLYTLRHLAYEQVQAIRFSRRSGDQQIDSIKRISALRKLIPGLHLVVGHDHTDY
                     HSKYLAPFLAKGWLSADERRAMAAYEACLFDANGRLPAPALPRFTPGHARRAPGTVSE
                     PKVGGTC"
     gene            6204..7058
                     /locus_tag="VGP50_05290"
     CDS             6204..7058
                     /locus_tag="VGP50_05290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8026820.1"
                     /translation="MPKRRAAPDSTAVRVALWRAMHVQVDPRPHVFEDDVGLKLAAPD
                     DGWRRRPDMDPRATSLPRAAIVARARFIEDLVAEQAGRGVGQYVILGAGLDTFAQRRP
                     ETVSGLRVFEIDRPGPQAWKRQRLIELGFGIPEWLRLVPADFETRGSWWERLRTAGFD
                     AGQPAVVASTGVSMYLTKDATTATLRQVAALTSGSTLAMTFLLPLEFAEPDERPVREA
                     AERGARAGGTPFISFFTPPEMLALARDAGFRDVQHVSAVTLTECYFAGRADGLRPAST
                     EALLVATT"
     gene            complement(7098..7454)
                     /gene="cutA"
                     /locus_tag="VGP50_05295"
     CDS             complement(7098..7454)
                     /gene="cutA"
                     /locus_tag="VGP50_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEV8026821.1"
                     /translation="MGRILSDSCYFVYVTARDAAEAKRIGRTVVAERLAACANVLDGM
                     SSVYWWQGKIEEATEAVLILKTTTKRFEELMARIKALHSYETPAIVAWPIAAGSAEYL
                     DWIGDETAPDARADAR"
     gene            7545..7847
                     /locus_tag="VGP50_05300"
     CDS             7545..7847
                     /locus_tag="VGP50_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026822.1"
                     /translation="MSAFRVSRKPTPSQVQRAWLRRGLDEPGGKLPLFDEYGKQIDPR
                     TIQACVDQGWAEPWFTNPIKPDWLVCKLTAAGRALALRGAAVSRVQAVHEIEKTSA"
     gene            7931..10690
                     /gene="ppc"
                     /locus_tag="VGP50_05305"
     CDS             7931..10690
                     /gene="ppc"
                     /locus_tag="VGP50_05305"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV8026823.1"
                     /translation="MDDPQQTADKDQPLRYDVRLLGRILGETVRAQEGDQVFERVERI
                     RQTALRFHRQADTTARHELQTIISGLAEGQAIRIIRAFGYFSHLANIAEDQHHIRRTR
                     AYAMANAAPREGTMARALTRARDAGVSRAALQGFFATALCSPVLTAHPTEIRRQSSID
                     REMEIAKLLDERDRVEFTPEELEANRTALRRAVLTLWQTSILRRTRLRILDEVANGLN
                     YYDQTFLREVPRLYADLEDQVAALTPDWDGLAVPAFLRMGSWIGGDRDGNPFVTADVL
                     SRALHMQSECAFRFYLEEVHRLGGELSLDGRMVRISDAVRRLVERSPDRSPQREDEPY
                     RRAIAGLYARLAATARGLDGIEPQRPEIAAAPAYASAGELLADLDALAESLAANGSSS
                     LARGRLRQLRRSVDVFGFHLASLDLRQNSDVHERVVAELLAMAGSSADYRALDEDARL
                     ALLLAEMRSPRPLGSPHLSYSETTVAELAILRAAAEGRRRYGNLAVPYYVISKAGAVS
                     DILEVAVLLKEVGLMRPWEGALDLDIVPLFETIDDLRRCGAVMDRLFALPEYRRLLEG
                     RGDQQEVMLGYSDSNKDGGYLTSTWELYKAERSLIDTFRRHGVRLRLFHGRGGSVGRG
                     GGPSYQAILAQPAGALQGAIRITEQGEVIAAKYANAEVGRRNLEALAAATLEATLLDS
                     DRAGPRAEYLAAMDELSSDAYEAYRGLVYETDGFERYFRESTVIGEIANLNIGSRPAS
                     RTGSPRIEDLRAIPWVFSWAQCRLMLPGWYGFGTAVERWLAAHPDGMAMLRRMYRDWP
                     FFITMLSNMDMVLAKSDIAIASRYAELVADPALRERIFGRLRAEWERSVRAVLEIMEQ
                     SVLLERNPLLARSIRNRFPYIDPLHHIQVDLLHRHRAGAADEKVVHAIHLTINGIAAG
                     LRNSG"
BASE COUNT         1642 a         3848 c         3630 g         2016 t
ORIGIN      
        1 cgtggtcttc ctcgatggac ttgatgaagc tgtcggccgc gagcggcgtt gccaggcagc
       61 taccaatgca aatcgcgctc aagatcccga gcagccaagc tctccgatgg cggcgttgct
      121 tcatctgcag accatagcag attgcgttca gcatcaacat tttgtataag cgtccagtgc
      181 gtcgcgtgtg acattgtcga ttgttttgcg gcacgtcacg gaaccacgtt tcctaagatc
      241 tgaaacatgc tgacccgcgc cggaaagcag ggaccgcgcg tggtcgctgc tttcagccga
      301 gactggccga cgaggccggg cccgacgatg ttctatggca tacccgtcat caccgatgta
      361 gccgcgagcg acgtgcgtct ccggccagcg gggtggcgag cgcccgcgcg cgccgagcga
      421 gcgttgaagt agggtaacgc cggggcacgg cgagggagag cacgctcgca aggtccgccc
      481 cttggagcaa gtctatggcc aatgccgttc cagcaccttt gaagccggca gcgctgtcgc
      541 tcaagcgcgc gggcctcgcc gccgcttttc tcgtcctcgc cctcatcctg ctgctgccgg
      601 cggcggcggg actgccggcc gccggccagc gcatgctcgc ggtcttcgcc ttcgccgtca
      661 tcgtctgggt gaccgaggcg ctcgattacg cggtgagtgc cgccgtgatc gccgcgctca
      721 tggcgttcct cctcggcact gcgccgaatg cggccaaccc gaaggcgctg ctcggcacct
      781 cggccggcct cgccacggcc gtgagcggtt tcggcagcac cgccttgacg ctggtcgccg
      841 ccgctctgtt cctgtccgct gccatgacca tcacggggct cgaccggcgc atcgctctcc
      901 tcgtgctgtc gaaggtcgca acgcggacga gccatgtcgt gatcggggcc atcgtcgtct
      961 cgacgctcct tgccttcctc gtgccgagct cgaccgcccg cgcggctgcg gtcgtcccca
     1021 tcatgatggg cgtcatcgcc gccttccgct tgccaaagag cagccggttc gccggcctcg
     1081 tcatgattac gaccgtgcag gccgtcacca tctggaacgt cggcatcaag acggcgtccg
     1141 cgcagaacat ggtcgctatc ggcttcatcc agaagctgct cgacaccgac gtcacctgga
     1201 tcagctggct cgtcgccgcg gcgcccttct cgatcgtgct ctcggtcgcg ctctatttca
     1261 tcatgatggc gatgatgccg cccgagacca cggagattgc cggcggccag gccagcgtgg
     1321 ccaaggcgct gagcgagctc gggccgatga cgggcgcgga gacgcgtctc ttgctggtct
     1381 cgctcgtcct gctcggcttc tgggcgaccg agggcgtgct tcacgcctat gacagcgcga
     1441 cgaccacgac gatcgccgtc gcgctgctgt tcctgccggg catcggcgtc atggattgga
     1501 agaaggcgag cgtcctcatc ccctggggga cggtcttgct cttcggcgtc ggcatcggtc
     1561 tcggcacggc gctgctccag acgcaagccg cgccatggct ggccaacatc atcgtggccc
     1621 ggttcggcct cgacgatctg gggccgctcg cgatcctggc ggtcatggcg gccttcctcg
     1681 tggtgattca cctgggtttc gccagcggca ccgcgctcgc ctcgtcgatg atcccgatcg
     1741 tcatcgccgt tctgcagaag gtccagacgc cgggcatcag cgtcgtcggc atgacgctgc
     1801 tgctgcaatt cgtcgtgagc ttcggcttca tcttgcccgt gaactcgccg cagggcatgg
     1861 tagcctatgg cacgggtacc ttctcggtgc gcgattacat ccgcaccggc gccgtcatca
     1921 ccgtcgtcgc ctatctgctg gtcctgctct tcagcgcgac ctactggcgc tggctcggct
     1981 atgtctgagg tgcggcttgg ccgattcgct catcgggcag gcccggcccg aggcgtgcga
     2041 tcatgaccga tcggccggcg ccgtcttcgc gacagccgat attcaacatt ccggcgggca
     2101 ccatggccct cctcatcatc aacatcgccg tgcatggttt gcggctgctg ctttccgatt
     2161 catcggacga ccgcatcgtc cgcgacttcg ccttcatacc ggcgcgttac acgacgggtc
     2221 tccttggctg gccggccttg gtcgatcccg tcacctatca gttccttcat gccaatttga
     2281 cccacctctt cgtcaacatg ctcgcgctgt tggccttcgg gtcgggcgtg gagcgtcgca
     2341 tcggtcgctg gcgaatgctc gcgctcgcga tcctgtcggg catcatcgcg gctggcgctc
     2401 acttggccgt ctatccatcg tccgacgtgc cggtggtcgg cgcatcgggc gccatcagcg
     2461 gtctgttcgg cgccgtgctg cggatgatgt cgcgcggcat ggccgggcgc cggagcggcc
     2521 tctggccgtt gatcgccttg tgggtcatcg tcacggtgat tgccggacaa accggcatgc
     2581 ccggtgaacc ggatgcgccc attgcctggg tcgctcatct cggcggcttc gccgccggcc
     2641 tcgcgctctt tggcttcttc gatcgcaccg cgggtcgtcg ctcggcatga ccgttgcgta
     2701 actcgctcga ctccggcgac atgctcgagc gccgagcgat tgctggaata tacggcggat
     2761 taaccattct gcatgagagt cgggattgcg cgtcgccact gtcgacggaa aggcaggggc
     2821 cgatgactcc caattcaacc cagcatgtta ccctcggcga tcccgtgccc gggttcaccg
     2881 cccgcacggt gaccggcgcg tcggtgtctt tgcacgtcga tgcgggacgc tggatcctcc
     2941 tctcgtttct cggctcgctc gccgaccagc gcgcggccga ggagcttgcc gccttgctgc
     3001 gcgaggcaag gcttttctcc gagccgcatc tcgtcgctta cgtcatcctg acggagccgc
     3061 cgcagcccga ggcgctgacg atgcttgcgg cggtgagcgg gcccgcgctc gccttcatcg
     3121 ccgattatga cggtgcgatc acccggagct tcggcgccga gcttatgcga cgcagcgtcg
     3181 tgctcgaccc gatgcagcgc gccatcgccg acattccctg ggaccacgcc gcgggtcatg
     3241 cggcgctgct ctgccaggtg ctgcgcgagc tgccgcccgt cgccgattcc gccggcgtgc
     3301 cgctctgggc gccggccctc atcgtgccgc gcgtcctcga tttcgcgctg tgcgatttcc
     3361 tcgtcaagct ttacgacgcg atgggcggcg aggaatcggg cttcatgctg gatcaggacg
     3421 ggaagaccgc ctcggtgatc gaccatcggc tgaagcgccg ccgcgacctg atcatcgtcg
     3481 acccggagct gcgcgagacc atccgcgggc agatcctgcg ccggcttatc ccggcgatcg
     3541 accagttctt cggcttcgcg gcgacgcgca tggatcgcta catcgtgtcc tgctacgaca
     3601 gcgccgtggg cggccatttc ttccgccacc gcgacaatgt gaacgccggc gcgcagcatc
     3661 gccggttcgc cgtgtcgatc aatctcaaca aggattacga gggctgcgac ctggtgtttc
     3721 cggaattcgg tccgcgcccc taccgccctc cggttggcgg cgccgtcgcg ttctccgccg
     3781 gcatgctcca cgaggtgacg ccgatcacgc gcggccggcg ccacgtcttc gtgcccttcc
     3841 tctatggcga agccgacgcc gcgcggcgcg agagcaacaa tgcccggctg cgcgacggag
     3901 aaagcctcta tgtcggcggc gccgaccgcc tcttcccgga ggcggcggaa taggtgtggg
     3961 ccacgatctg gcggcgctcg agcgcttcac gccggtcgcg ctcaacctcg acgcagaacc
     4021 ttcgatcgat tggggcgatc tcgacggggt gcgcttcagc gagccgttct tcgaccagac
     4081 gatcgagcgc tgggccggcg gcccggcgcc gcgcctggtg cgcaccggcc tcgatgcgct
     4141 tgcggcgctc gacgcggcgc cttcgctcga tcctgcggcg ctcatcttcc atctgtcgcg
     4201 ctgcggctcg acgctggtgt cgcgcctgct cgcgacccat ccacggacgc tggtgatctc
     4261 cgagccgaag ccgctcaatg cgctcctcat ggccggtccg ccggagttcg acgccgagcg
     4321 gcaggcatac ctcctccgcc tgctggtgcg cgcgctcggt cgccgccgga cggggggcga
     4381 gcggcactac gtgctgaagc tctcgagctg gaatgtcacg cggctcgccc ttttccgccg
     4441 cgcttttccg gacgcgccgc tggtctggct gcagcgcgcg ccgagcgaga tcatggcctc
     4501 gatcagtgcc gacccgccgg gctggatgca gctgcgccac gtcccgtcgc aggcagaggc
     4561 gctgttcggc attcccgcgg ccagcagcgc cggccttgat gccgaggaat tctgcgcgcg
     4621 cgccctcgcg gcgatgctgg cggctgcccg cggggccgaa gatgccggcg cgttgctggt
     4681 ggactatcgc gagctcccgg acgcggtatg gcagcgcgtc gcgccctttg ccggtatcgc
     4741 gctcgacgac gtcgcgacgg ggcggatgcg agaggaagcg cgcttccatt ccaaggacac
     4801 cgcgcgacgg ctgttcaccg gcgaccctgc cgagacgcgt cccgcctcgg atcgcgccca
     4861 tgcgctcgtc gccgacctgg ccgagccgct ctaccaggcg ctcgacaggc gcaggcgcgc
     4921 ggcgccggac gtctgacgac gatcgcgcgg ccggcagtgg tccagcaatt cacttcccag
     4981 agaagcatgg acggttgacg cgcgatcgca tattcgatgc ggacgtttgc tcgggcgccg
     5041 gcacgcggca gcacggccgg gtttgcgcca ggacgggaga catcgatgaa gctctttcct
     5101 ctgttgctcg gccgggcaaa ggtgccgtat ggccagttct atggcggcct ttcgggctgg
     5161 caggggctgg gtgcgctgct tcgctacgtc ttcgacaaaa gccacttcat cgaggtgccg
     5221 atccacgcgt atctgctcga gcatccgcgc gagggattgg tcctgatcga tgcgggtatc
     5281 tgtccggaac aagcgcatca ccaccgtcag tactatcgcg gcctgctggg catgaccctc
     5341 aacgacgacg aataccgcca ggccgcgaac gagaccttgc aggagcagct gaagcccctg
     5401 ggtcatagcg tcaacgatat cagaaccgtc atattgaccc atctccacga cgaccacgct
     5461 ggcgggctcc gccagctccc taaggcgaaa gtcgtgatcg ccaagaccga atgggaggcg
     5521 cggcacatga aactcttggg cttcatcccg atgttttacg agccgtcctt cggcgccgtg
     5581 gcgcagtggc agtatgtgag cttcgaatcg agcgcgttcc actccttcga tcaaagcgag
     5641 gacctgttcc gcgacggctc ggtccgcctg ctgccgacgc cgggacacac gcccggccac
     5701 acctccgccc tgatcgatat gggcgagtat cggctcctgg tcaccggaga ctgcctctac
     5761 accttgcggc acctggccta tgagcaggtt caggcgatcc gcttctcgcg ccggagcggc
     5821 gaccagcaga tcgattcgat caagcgcatt tccgcgctca gaaagctgat ccccggcctt
     5881 catctcgtgg tcggccatga ccatacggac tatcattcga agtatctggc gccgttcctg
     5941 gcaaagggct ggctcagcgc ggatgagcgg cgggcaatgg cggcttatga agcgtgcttg
     6001 ttcgacgcca atgggaggct ccccgcccct gccctgccgc ggttcacgcc cggtcacgcc
     6061 agaagagcgc ccggaacggt cagcgagccg aaggtgggtg gaacctgctg agggcgggcg
     6121 tgcgcgctgt tacgcatggc tcgaaaatcg ccctgacgac aatccgtttg tcgcggaacc
     6181 cgctccaaag aagggagcac atcatgccga agcgaagagc ggcgccagac agcacggcgg
     6241 tccgcgttgc cttgtggcgg gccatgcacg tccaggtcga tccgcggccg catgtgttcg
     6301 aagacgacgt cggcctcaag ctggcggccc cggacgatgg ctggcgccgc cgcccggaca
     6361 tggacccacg cgccacgagc cttccccgcg ccgccatcgt ggcccgagct cgcttcatcg
     6421 aggatctggt cgcggaacag gctggccgcg gcgtcgggca gtacgtcatc ctcggcgccg
     6481 gcctggacac cttcgcccag cgcaggccgg agacagtctc cggcctgcgg gtattcgaga
     6541 tcgatcgtcc cggtcctcag gcgtggaagc ggcagcgcct gatcgagctc ggcttcggca
     6601 tccccgagtg gctgcgtctc gtgccggccg acttcgagac gcgcggttcg tggtgggagc
     6661 ggctcaggac cgccggcttt gacgccggcc agccagccgt cgttgcctcc accggcgtca
     6721 gcatgtatct gacaaaggac gcgaccaccg ccacgttgcg ccaggtggcg gcgcttacct
     6781 caggctcgac gctggccatg acgttcctcc tgccgctcga attcgccgaa ccggacgagc
     6841 gtccggtgcg tgaggcagcg gagagagggg cacgcgccgg cgggacgcct ttcatcagtt
     6901 tctttacccc gccggagatg ctggccctgg cccgggacgc cggtttcaga gacgtgcaac
     6961 acgtgtcggc cgtcactctc acggagtgct acttcgccgg cagggcggac ggccttcggc
     7021 cagcaagcac ggaggcgctc cttgtagcga cgacttagcg accgcgcgct tcggccgcga
     7081 gcgctgcctc gtccccctca gcgcgcgtcg gcccgcgcgt cgggagcggt ctcgtcgccg
     7141 atccaatcga gatactccgc gctgccggcc gcgatgggcc aggcgacgat ggccggggtc
     7201 tcgtaggaat ggagcgcctt gatgcgggcc atgagctctt cgaagcgttt ggtcgtcgtc
     7261 ttgaggatga gcaccgcttc cgtggcctcc tcgatcttgc cttgccacca atagaccgag
     7321 ctcatgccgt cgagcacatt ggcgcatgcc gccagccgct ccgcgacgac ggtgcggccg
     7381 atgcgcttcg cctcggccgc gtcgcgggcg gtgacataga cgaaatagca gctgtcgctg
     7441 agaatgcgcc ccatgatgcg ctcccgtctc cgattcggct ggccctcgcc ggcgattccc
     7501 gcttaacatc gcaaacacgc aaagtcgagg gaggaggccc cgcgatgagc gcgtttcgcg
     7561 tcagccgcaa gccgacgccg agccaggtgc agcgcgcctg gctgaggcgg ggtttggacg
     7621 agcccggcgg caagctgccg ctcttcgacg aatacggcaa gcagatcgac ccgcgcacca
     7681 tccaggcctg cgtcgatcaa ggctgggcgg agccctggtt caccaatccc atcaagcccg
     7741 actggctcgt ctgcaagctg accgcggcgg gccgcgcgct cgcgctccgc ggcgcagccg
     7801 tttcgcgtgt tcaagcagta catgaaatag agaagacaag cgcctgatct tgcgagttct
     7861 ccgcacgtgt ttttgagccg cgggaccgcg tcgtcgatgc gtgccagggc tgcgcgcgag
     7921 gagagcggcg atggacgatc cgcagcagac cgccgataag gaccagccgc tccgctacga
     7981 cgtcaggctc ctcggccgca tccttggcga aacggtgcgc gcccaggaag gcgatcaggt
     8041 cttcgagcgc gtggagcgca tccggcagac cgcgcttcgc tttcatcgtc aggccgacac
     8101 gacggcgcgc cacgagctcc agaccatcat ttccggcctc gccgagggtc aggcgatccg
     8161 catcatccgc gccttcggct atttctcgca tttggccaac atcgccgagg atcagcacca
     8221 tatccgccgt acgcgcgcct acgccatggc caatgccgcg ccgcgcgagg gaacgatggc
     8281 gcgtgccttg acgcgcgcgc gcgacgcggg cgtgtcgcgg gcggcgcttc aagggttttt
     8341 cgccaccgcg ctgtgcagcc cggtcctgac ggctcacccg accgagattc gccgccagag
     8401 ctcgatcgat cgcgaaatgg agatcgcgaa gctgctcgat gagcgcgatc gggtggagtt
     8461 cacgcccgag gagctggaag cgaaccgcac ggcccttcgc cgcgccgttc tcacgctgtg
     8521 gcagaccagc attctgcgcc gcacccggct gcgcatcctc gatgaggtcg cgaacggcct
     8581 caactactac gaccagacct ttcttcgcga ggtgccgcgc ctctacgccg atctcgaaga
     8641 ccaggtggcg gcgctcacgc ccgactggga cggactcgcg gttccggcct ttttgcggat
     8701 gggcagctgg attggcggcg accgcgacgg caaccccttc gtcacggccg atgtgctgag
     8761 ccgggcgctc cacatgcaga gcgaatgcgc ctttcgcttc tatctcgagg aggtccaccg
     8821 attgggcggc gagctgtcgc tcgacggccg catggtgcgc atctcggacg cggtgcgtcg
     8881 cctggtcgag cggtcgcccg accgctcgcc gcagcgtgag gacgagccct accggcgtgc
     8941 gatcgccgga ctctatgccc gcctggcggc gacagcccgc gggctcgacg gcatcgagcc
     9001 gcagcggccc gagatcgctg cggcaccggc ttatgccagc gccggcgagc ttctcgcgga
     9061 cctcgacgct ctcgcggaat cgctcgccgc caacggctca tccagtctgg cccgcggccg
     9121 attgcggcag ctccgccgca gcgtcgatgt cttcggcttt catctcgcct cgctcgacct
     9181 gcggcagaac tcggacgtcc atgagcgcgt ggtcgccgag ctcctcgcca tggcgggatc
     9241 gagcgcggac taccgcgcgc tcgacgagga tgcgcgcctc gcgctgcttc ttgcggagat
     9301 gaggtcgccc cgcccgctcg ggtcgcccca cctgtcctat tccgagacca cggtcgccga
     9361 gcttgccatc ctgcgcgctg cggccgaagg gcgacggcgc tatggcaacc tcgccgttcc
     9421 ttactacgtc atctccaagg cgggcgccgt gtcggacatt ctcgaagtgg cggtgctgct
     9481 caaggaggtc gggctcatgc gtccctggga gggcgctctc gacctcgaca tcgtccccct
     9541 cttcgagacc atcgacgatc ttcgccggtg cggcgccgtc atggaccggt tgttcgcgct
     9601 gcctgaatat cgccggctcc tcgaggggcg cggcgatcag caggaggtga tgctcggcta
     9661 ttccgacagc aacaaggacg gcggctatct cacctcgacc tgggagctct acaaggcgga
     9721 gcgcagcttg atcgatacct tccggcgtca cggcgtccgg ctgcgcctct tccatggacg
     9781 cggcggctcg gtcggccgcg gcggcggacc gagctatcag gcaattctcg cccagccggc
     9841 gggcgcgctg cagggcgcga tccgcatcac cgagcagggc gaggtgatcg ccgccaaata
     9901 tgccaacgcc gaggtcggcc ggcgcaatct cgaggcgctc gccgcggcga cgctcgaggc
     9961 gacgttgctc gactccgacc gggcaggccc gcgcgccgag tatctggcgg cgatggacga
    10021 gctttcgagc gacgcctatg aggcatatcg gggcctcgtc tacgagacgg acggcttcga
    10081 acggtatttc cgcgaatcga cggtcatcgg cgagatcgcc aacctcaata tcggcagccg
    10141 gccggcctcg cgcaccggct cgccgcgcat cgaggatttg cgcgcgattc cctgggtctt
    10201 cagctgggcg cagtgccggt tgatgttgcc gggctggtac ggcttcggca cggcggtcga
    10261 gcgctggctc gcggcgcatc ccgacggcat ggccatgctg cgccggatgt accgcgattg
    10321 gcccttcttc atcaccatgc tgtcgaacat ggacatggtc ctggcgaaaa gcgatatcgc
    10381 gatcgcgtcc cgctatgcgg agctggtggc ggatccggcg ctgcgcgagc gcattttcgg
    10441 ccgcctgcgc gccgagtggg agcggtccgt gcgcgccgtt ctcgagatca tggagcagag
    10501 cgtgctgctc gagcgcaatc cgctgctcgc ccgttcgatc cgcaaccgct tcccctacat
    10561 cgacccgctc caccatatcc aagtcgatct gctccatcgc caccgcgccg gcgcggcgga
    10621 cgagaaggtg gtccatgcca tccatctgac gatcaacggc atcgccgcgg gcttgcgcaa
    10681 cagcggatag tcgttgacgt cgcgcttttc tcgatccacg ccgctgcgcg ggcggtagag
    10741 tctgtcctac acatcgaccg aaagctgcga tcggcagagc gcggcctatt ggcagcgcgt
    10801 cgagcgctgc caggctgcgc tcctgctgga tcgctgacgg tgcgacttag agtgaccgcc
    10861 ccggagcgac atgcccgcgc gccggcttct cgctcctagg cttgtcgacg acgctcaggc
    10921 ttgcggtgaa caccggccga agactttgtc cggcgcttga tttgtccttg cgattggctt
    10981 gattattcct tgagagaccc gtgccatccg ctgagcgccc cgacatgcgg tttgtctttg
    11041 ctggccatac gctcgatacc gaccggcgcg agctctgccg cggttcggag gcgatcgccg
    11101 tcgagccgca ggtcttcgat ctcctgacct atctgc
//
LOCUS       DASYNS010000168         3761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2221439, whole genome shotgun sequence.
ACCESSION   DASYNS010000168 DASYNS010000000
VERSION     DASYNS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221439"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..2305)
                     /locus_tag="VGP50_05310"
     CDS             complement(47..2305)
                     /locus_tag="VGP50_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV8026824.1"
                     /translation="MSDPPPPAAATETPRPYLAGLNEGQRQAVEAIDGPVLVLAGAGT
                     GKTRVLTTRLAHVLATRRAWPSQILAVTFTNKAALEMRERLERMIGEAAEGLWLGTFH
                     SIGARILRRHAEGVGLKSNFTILDADDQLRLVKQLAQAEGVDDKRWPARMLLGAFERW
                     KDRGLIPDKVPAGERGDLANGRGLKLYRQYQERLVTLNAADFGDLLLHNLTIFAERAD
                     ILAEYQKRFHYILVDEYQDTNVAQYLWLRLLAQGHRNLCCVGDDDQSIYSWRGAEIGN
                     ILRFERDFPSARIVRLEQNYRSTPHILAAASGLIAHNEGRLGKTLWTAVNEGERIVLR
                     GLWDGPEEARWVGDAIEARQRQGERLSEIAILVRASFQTREFEERFLTLGLPYRVIGG
                     PRFYERQEIRDALAYLRLIHQPADDLAFERIVNLPRRGIGGATLQSLHGVARAADIPL
                     AEAAARLVETDEVKPAARQALRDFLATLSRWRGLAQTAAHTDLAGIVLDESGYTAMWQ
                     ADKSPDAPSRLENLKELVEAMAEFENLAGFLEHVSLVMETTRGSAGDMVSLMTLHGAK
                     GLEFDTVFLPGWEEGLFPNQRAMQETGRAALEEERRLAYVGLTRARHRAYVSYAANRS
                     IHGQWQATLPSRFLAELPGAHVEVVADPGLYPRSTVFDRQDWSKATPRPDLAHLGPLG
                     RRAPLMERQPAPRGGRELRHAGGFSAGDRIFHQKFGYGTVRTVDHNKLAITFDKAGDK
                     MVMDSFVERATE"
     gene            2481..3737
                     /locus_tag="VGP50_05315"
     CDS             2481..3737
                     /locus_tag="VGP50_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV8026825.1"
                     /translation="MPAIIVTLVFLFTYAGMAAGRLPWLQVDRTGIALLGVIALLVTE
                     TVTLDDLGATIDTSTLVLLFALMIISAQFVSSGFYDLCADWITGRRGGAAWLLAITVA
                     VCGGITALLANDIVVLTMTPLLIAGARSRGLDPRPFVIALAGASNAGSAATVIGNPQN
                     ILIGQVGNLSFHTFLLVCGVPAVVGLVCVFGVVWLLWHDRIADTVMAVTREVSDVPRH
                     PHDRNQTIKGLVALVMLLVLFATPLPREIGGLVIAALLLANRKFTSRTMIAAVDWPLL
                     LLFVCLFAITGSFANQTSLPAAISSGLEAIGLAPESLAVLTPLSLAMSNTIGNVPSVI
                     LLLQLWPNLPEGALYGLALLSSLAGNLLLIGSLSNLLIVERAAAFGVRIGFAEYARAG
                     IPIALLSMAFAVFWLAVTGWLPVLPE"
BASE COUNT          542 a         1271 c         1293 g          655 t
ORIGIN      
        1 acggaatgat ccacaggcga acgcgctttg cgcgttcgcc tgtggatcat tccgtcgcgc
       61 gttcaacaaa tgaatccatg accatcttgt cgccggcctt gtcgaaggtg atggcgagct
      121 tgttgtggtc gacggtgcgc acggtgccgt agccgaattt ctggtggaaa atgcggtcgc
      181 ccgcgctgaa gccgccggcg tggcggagct cgcgaccgcc gcggggcgcc ggctggcgtt
      241 ccatcagcgg cgcgcgccgg ccgaggggcc cgagatgcgc gagatcgggg cgaggcgtgg
      301 ccttggacca gtcctgacga tcgaaaaccg tcgagcgcgg atacagcccg ggatcggcga
      361 cgacctcgac atgcgcgccg ggcagctcgg cgagaaagcg cgacggcagc gtcgcctgcc
      421 actgtccgtg gatgctgcga ttggccgcgt aggagacata ggcgcggtgg cgcgcccggg
      481 tgaggccgac ataggcgagg cggcgctcct cttcgagcgc ggcgcggccg gtctcctgca
      541 tggcgcgttg attggggaaa agcccctcct cccagccggg caggaaaacc gtgtcgaatt
      601 cgagcccctt ggcgccgtgg agggtcatca ggctcaccat gtcgccggcg ctgccccgcg
      661 tcgtctccat caccaggctc acatgctcga ggaagcccgc gaggttctcg aactcggcca
      721 tggcctcgac cagctccttg aggttctcga gccggctcgg ggcgtcgggc gacttgtcgg
      781 cttgccacat cgcggtatag ccgctctcgt cgaggacgat gccggcgaga tcggtgtgcg
      841 cggcggtctg ggccaggccg cgccagcgcg acagggtcgc tagaaaatcg cgcagcgcct
      901 gccgggccgc cggtttcacc tcgtcggtct cgaccagccg cgccgccgcc tcggcgagcg
      961 ggatgtccgc cgcgcgggcg acgccgtgca gcgattgcag cgtggcgccg ccgatgcctc
     1021 gccgcggcag gttgacgatg cgctcgaagg cgagatcgtc tgccggctga tggatcagcc
     1081 gcagataggc gagcgcgtca cggatctcct gccgctcata gaagcgcggg ccgccgatca
     1141 cgcggtaagg caggccgagg gtgaggaagc gctcttcgaa ttcgcgggtc tggaagctgg
     1201 cgcgcaccag gatggcaatc tccgagagac gctcgccctg gcgctgccgc gcctcgatcg
     1261 cgtcgccgac ccagcgcgcc tcctcgggcc cgtcccagag gccgcgcagg acgatgcgct
     1321 cgccctcatt caccgcggtc cacagcgtct tgccgagtcg cccctcgtta tgggcgatga
     1381 ggcccgacgc ggcggcgaga atatgcggcg tcgagcgata gttctgctcg agccgcacga
     1441 tccgcgcgct cgggaagtcg cgctcgaagc gcaggatgtt gccgatctcg gcgccgcgcc
     1501 aggaatagat cgactgatcg tcgtcgccga cacaacagag gttgcgatgc ccctgagcca
     1561 aaaggcgcaa ccacaggtat tgcgcgacgt tggtgtcctg atactcgtcg accaggatgt
     1621 agtgaaagcg cttctgatac tcggccagga tgtcggcgcg ctcggcgaag atcgtcaggt
     1681 tgtgcagcag caagtcgccg aaatcggcgg cattgagggt gacgaggcgc tcctggtatt
     1741 ggcgatagag cttgaggccg cggccattgg cgaggtcacc gcgctcgccc gccggcacct
     1801 tgtcggggat gaggccgcgg tctttccagc gctcgaaggc gccgagcagc atccgcgcgg
     1861 gccagcgctt gtcgtcgacg ccctccgctt gggccagctg cttgacgagg cggagctggt
     1921 cgtcggcatc gaggatggtg aaattggact tgagcccgac cccttcggcg tgccgccgca
     1981 ggatacgcgc gccgatcgaa tggaaggtgc cgagccacag cccctcggcg gcctcgccga
     2041 tcatgcgctc gagccgctcg cgcatctcga gcgccgcctt gttggtgaag gtcaccgcca
     2101 ggatctggct cggccaggcg cggcgcgtgg ccaggacgtg agcgagccgg gtggtcagga
     2161 cgcgcgtctt gccggtaccg gcgccggcca ggaccagcac cggcccgtcg atcgcctcga
     2221 ccgcctggcg ctgcccttca ttgagcccgg cgagataggg ccggggcgtc tcggtcgcgg
     2281 cggcgggagg cggagggtcg gacattgctc ggcgtatata gcgcgatgcg gcgagggggc
     2341 gaagagctga agaggctagc gcgagcgcag agcgctcgtc gcaggcggac gaacgcgcaa
     2401 agcgcgttcg ccgcgcgaaa tttctggagc ctttccgaac ggacccccta tgatggcgcc
     2461 ggccgatcga gccgtccttc atgcccgcca tcatcgtcac cctcgttttc ctcttcacct
     2521 atgccgggat ggcggccggc cgcctgcctt ggctgcaagt ggatcgcacc ggcatcgccc
     2581 tcctgggcgt gatcgccctt ctggtgaccg aaacggtgac gctcgacgac ctcggcgcga
     2641 cgatcgacac gtcgacgctg gtgctgcttt tcgcgctcat gatcatctcg gcgcagttcg
     2701 tgtcctccgg tttctatgat ctctgcgccg attggatcac cggccggcgc ggcggcgcgg
     2761 cctggctgct cgccatcacg gtcgcggtgt gcggcggcat caccgcgctc ctcgccaatg
     2821 acatcgtcgt gctgacgatg acgccgctgc tgatcgcggg ggcgcgcagc cgcggcctcg
     2881 atcccagacc cttcgtcatc gccttggcgg gggccagcaa tgccggctcg gccgcgaccg
     2941 tcatcggcaa tccgcagaac atcctcatcg gccaggtcgg caatctgagc ttccacacct
     3001 ttctcctggt ctgcggcgtg ccggcggtgg tcggcctcgt ctgcgtcttc ggcgtggtgt
     3061 ggctgctctg gcatgaccgc atcgcggaca ccgtcatggc ggtgacgcgc gaggtctcgg
     3121 acgtgccgcg ccatccccac gaccgcaacc agaccatcaa gggcctggtc gccctggtca
     3181 tgctgctcgt gctctttgcg acaccgctcc cgcgcgagat cggcggcctg gtgatcgccg
     3241 cgttgctgct cgccaaccgc aaattcacca gccgcacgat gatcgcggcg gtcgattggc
     3301 ccttgctgct gctcttcgtc tgcctcttcg cgatcaccgg gtcgttcgcc aaccagacct
     3361 cgctgccggc ggccatcagc tcgggcctcg aggcgatcgg gcttgcgccc gaaagcctcg
     3421 ccgtgctgac gccgctgtcg ctcgccatga gcaacacgat cgggaacgtg ccctcggtga
     3481 ttctgctgct gcagctctgg cccaatctgc cggaaggcgc gctttacggc ctggcgctgc
     3541 tgtcctcgct cgccggcaac ctgctgctca tcggcagcct gagcaatctc ctgatcgtcg
     3601 agcgcgccgc cgctttcggc gtgcgaatcg gctttgccga atacgcccgc gccggcatcc
     3661 ccattgcgct gctgtcgatg gcgttcgcgg tcttctggct cgcggttacc gggtggttgc
     3721 cggtgctgcc cgagtgaacg cgcctttcgc gttcactcgg g
//
LOCUS       DASYNS010000169         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3937270, whole genome shotgun sequence.
ACCESSION   DASYNS010000169 DASYNS010000000
VERSION     DASYNS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3937270"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VGP50_05320"
     CDS             complement(<1..838)
                     /locus_tag="VGP50_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV8026826.1"
                     /translation="MAVLVTGAAGFVGFHTSAALLARGETVIGIDNLNPYYAVSLKEA
                     RLAELTPRRGFAFYRLDIAERGAVTALVQQHPEIDRVVHLAAQAGVRHSLIDPHAYIA
                     ANIQGQLEILEACRRVPRLAHLVFASSSSVYGGNTKLPFAISDPVDTPQSLYAASKKA
                     DELMSYCYAHLYRLPSTGLRFFTVYGPWGRPDMAAWLFTEAILKGEPIRVFNNGAMRR
                     DFTYIDDIVAGVLAALDHPPSDSGKEPPYRLYNLGNHRSEELKRFIAVLEQAIGREAI
                     IEL"
     gene            942..1997
                     /locus_tag="VGP50_05325"
     CDS             942..1997
                     /locus_tag="VGP50_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEV8026827.1"
                     /translation="MKSYDVVDYGAPLRKVERPTPQPQGTEVLLRTLAAGVCHSDLHI
                     HEGWYDLGGGKKLMAKDRGFLPPITMGHEIVGEVVAVGSEASGVKIGDKRLAHPWIGC
                     GSCAVCRRGTEQLCLTPRFLGIHRPGGYADHVLVPHPRYLLDIGKLTPAQAAPYACSG
                     ITTYGALKRVGEALYREEPILVIGAGGLGLMCLTLLKALGGRGAVVADIDPKKRQAAL
                     AAGALAAVDAAAPDAAKQAQEAAEAPLAAAIDFVGASATARLGIDAIARGGKYVIVGL
                     FGGDITLSLPFLPMRAITIQGSYVGSLAEIKELMALVGTGKVAPMPVSERPLDDANAV
                     LEELRGGRITGRAVLRP"
BASE COUNT          335 a          646 c          743 g          317 t
ORIGIN      
        1 cgagctcgat gatggcctcg cggccgatcg cctgctcgag cacggcgatg aagcgcttga
       61 gctcttcgct ccggtgattg ccgagattgt agagacgata gggcggctcc ttgccggaat
      121 cgctgggcgg atggtcgagg gccgcgagca cgcccgcgac gatgtcgtcg atgtaggtga
      181 agtcgcgacg catcgcgccg ttgttgaaaa cgcggatcgg ctctcccttg aggatcgctt
      241 cggtgaagag ccaggccgcc atgtccggtc gcccccaggg accgtagacg gtgaagaagc
      301 gcaagcccgt gctcggcaga cggtagagat gggcatagca gtagctcatc agctcgtcgg
      361 ccttcttgct ggcggcgtag agcgactgcg gcgtgtcgac cggatcgctg atggcgaagg
      421 gtagcttggt gttgccgcca tagacggagg acgagctggc gaagacgaga tgggcgaggc
      481 gcggcacccg ccggcaggct tcgaggattt cgagctgccc ctggatattg gcggcgatgt
      541 aggcgtgggg atcgatcagc gaatggcgca cgcccgcctg cgccgccaga tgaacgacgc
      601 ggtcgatctc gggatgctgc tggacgagcg ccgtcacggc gccgcgctcg gcgatgtcga
      661 gacgatagaa ggcgaagccg cgccgcggcg tgagctcggc gagccgggcc tctttgagag
      721 agaccgcgta gtaggggttg agattatcga tgccgatcac ggtctcgccg cgcgccagca
      781 gagcggcgct ggtgtggaat ccgacgaagc cggcggcgcc ggtgacgagg acggccatag
      841 gccgcggtat agcccggcgc ggccgtgaaa cacagccgga agttggcaca agcggcgtct
      901 ctcctttaag cttcgccatc cggatgcaag gaggagccgc catgaagagt tatgacgtcg
      961 tcgattacgg tgcgccgctg cgcaaggtcg agcggccgac gccgcagcct caggggaccg
     1021 aggtgctgct gcggacgctg gccgccggcg tgtgccatag cgatctccac atccacgaag
     1081 gctggtacga tctcggcggc ggcaagaagc tcatggcgaa ggaccgcggc ttcctgccgc
     1141 cgatcaccat gggccacgag atcgtcggcg aggtcgtggc ggtcggatcc gaggcgagcg
     1201 gcgtcaagat tggcgacaag cgcctggcgc atccctggat cggatgcggc agctgcgccg
     1261 tttgccggcg cggcaccgag cagctctgcc tgacgccgcg gttcctcggg atacaccggc
     1321 ccggcggtta tgccgaccac gtgctcgtgc cgcatccgcg ctatctgctc gatatcggca
     1381 agctcacgcc ggcgcaagcg gcaccttatg cgtgttcggg catcaccacc tatggcgctt
     1441 tgaaacgggt gggagaggcg ctctaccgcg aggagccgat cctcgtcatc ggcgctggcg
     1501 gcctcggcct catgtgcctg acgctgctca aagcgctggg cgggcgcggc gcggtcgtcg
     1561 ccgacatcga cccgaagaag cgccaggcgg cgcttgcagc gggagcgctc gccgctgtcg
     1621 acgcggcagc tcccgatgcg gcgaagcagg cgcaggaagc cgccgaggca ccgctcgccg
     1681 ccgccatcga cttcgtcggc gccagtgcca cggcgcggct cggcatcgat gccatcgcgc
     1741 ggggtggaaa atacgtgatc gttggcctct tcggcggcga catcaccttg agcctgccgt
     1801 tcttgccgat gcgcgccatc acgatccagg gctcctatgt cggcagcctc gcggagatca
     1861 aggagctgat ggcgctggtc ggcaccggca aggtcgcgcc catgccggtg agcgagcggc
     1921 cgctcgacga cgcaaatgcc gtgctcgaag agctgcgcgg cgggcgcatc acgggccgcg
     1981 cggtgctgcg cccttaaccc gactcggccg tcgccgcggc cagaggcgcg ggcgcccccg
     2041 a
//
LOCUS       DASYNS010000170        11105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2846378, whole genome shotgun sequence.
ACCESSION   DASYNS010000170 DASYNS010000000
VERSION     DASYNS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11105
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2846378"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VGP50_05330"
     CDS             complement(<1..417)
                     /locus_tag="VGP50_05330"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388353.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV8026828.1"
                     /translation="MANAIRALAMDAVEQANSGHPGMPMGMADVATVLWSRFLKFDPA
                     RPDWPDRDRFVLSAGHGSMLLYALLHLTGYPGMTIEELKRFRQLGSRTAGHPERGHAQ
                     GIETTTGPLAQGLGSAVGMALAERLLAARFGAALVDH"
     gene            743..817
                     /locus_tag="VGP50_05335"
     tRNA            743..817
                     /locus_tag="VGP50_05335"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:775..777,aa:Gln,seq:ttg)
     gene            834..1262
                     /locus_tag="VGP50_05340"
     CDS             834..1262
                     /locus_tag="VGP50_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026829.1"
                     /translation="MNMKRTFYRRSVVLVLLGLAACGMQTVQNESLDTAETQSFDASY
                     RDVVQAARDGITTAGFDITQAEEKDTGFVLLFVRPASYGQFGAVGRLVVDRSDAPPTA
                     VHVNYERRVLIYGAGQERAARRIFKRMQETLRDKGVSPKS"
     gene            1348..1731
                     /locus_tag="VGP50_05345"
     CDS             1348..1731
                     /locus_tag="VGP50_05345"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV8026830.1"
                     /translation="MKMHNPATVAAPTGPFSHGVEVPPNARWLCVSGQIGVAPDGSTP
                     ADLEGQAEQCWRNVKAILAVAGMGVEHLLKCTHFLTRAEDVAAYGKVRARHLGEARPA
                     STLLVVAALARPGLLVEVEAVAAKA"
     gene            complement(1831..2388)
                     /locus_tag="VGP50_05350"
     CDS             complement(1831..2388)
                     /locus_tag="VGP50_05350"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HEV8026831.1"
                     /translation="MDDGAPNLPDLLDDGLRVLFVGINPSIFSARRGHYFARPSNRFW
                     PCFSRSTLSLAARRALGVSELRPEHDRALLAQGFGFTDLVKRATVRASEVADDEFVAG
                     IARLEAKLARHRPSFACFHGMTGYRHVHRALAADAPPALGLQSARIAGTALFVVPSPS
                     GANAHFSRAEQTQWYDRLADALSQN"
     gene            2577..2732
                     /locus_tag="VGP50_05355"
     CDS             2577..2732
                     /locus_tag="VGP50_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026832.1"
                     /translation="MADAAYFREKAQQCRELLKHAIEPEVIEQLEIWVREFEERARIA
                     QREASRD"
     gene            2885..3955
                     /locus_tag="VGP50_05360"
     CDS             2885..3955
                     /locus_tag="VGP50_05360"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV8026833.1"
                     /translation="MPTQRAALFVLTTLLVVLGLWILRHFLPALAWAGVLAIGTWPLY
                     SRFRRLLPPRLQPSTWAPLGFTLLVGVVFVLPLTLFAIEVGREALVLVHQVGAIEQTG
                     MPPPDWLAHLPFGSYATAWWQNNLSDPQAAQELMGRVDRSVVLEWLRSLGLQLFARST
                     ILVFTLLALFFLYRDGLTLAHQLAALAARTFGPTGEDLGDHVIAAVRGTVDGLVLVGL
                     GEGLLLGAGYAVLGLPHALLLGAITCVLAMIPFGAPVIFGLGSLFLLAQSGVAAAVGL
                     AAYGVVISFIADHFVRPVLIGGAVRLPFLWVLLGILGGVESFGLLGLFLGPAVMAALI
                     ALWREWTAHAGGEGKEDSPMRP"
     gene            complement(4114..5712)
                     /locus_tag="VGP50_05365"
     CDS             complement(4114..5712)
                     /locus_tag="VGP50_05365"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV8026834.1"
                     /translation="MTAKRRKFFGWGLEGEGLTAAEDAFGLERYRKLFGVTRFDERPP
                     PRLPDLALRPPRLSPPPALAAFCSSATHDRAVHAYGKSFPDYVRGLAGDYANAPDVVA
                     YPRNEAEVAAVLDWAGGARAAIVPFGGGSSVVGGVEPRLDGGRYKAALTLDLGRMDRV
                     REVDRASRAARIEGGVFGPALEAQLKPHELTLRHFPQSFEYSTLGGWIATRSGGHFAT
                     LLTHIDDLVESLRVVTPRGVVETRRLPGSGAGPSPDRLFIGSEGTLGVITEAWMRLQD
                     RPRFRAGGAVGFKDLFTAAQAVRMIAQAGLYPANCRILDPQEAYNTGAGDGRVAIMVL
                     AFESGDHPVDAWMKRALECCADHGGTPEDTSGAEAHRDGAAGVWRNAFIRMPYARERM
                     VPRAIISDTFETAITWDRFEVFHDKVKSATEQAIRAATGRPGQVTCRFTHVYPDGPAP
                     YFSFHGLGRHGHLIEQWQAIKTAASDALIAGGGTITHHHAIGRDHRPWYDRQRPDLFA
                     AALKAAKVALDPQGLLNPGVLIDP"
     gene            complement(5816..6121)
                     /locus_tag="VGP50_05370"
     CDS             complement(5816..6121)
                     /locus_tag="VGP50_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEV8026835.1"
                     /translation="MRPGIGAREAAHVAEIEQWAWRRVRSLRGFYSHLTAYVIINFVL
                     FLIDSTTPGPAWFYAPLIGWGMLVLLHGLHAHELLPWTTQDWEQRKVRELIESRLRR"
     gene            complement(6365..6835)
                     /locus_tag="VGP50_05375"
     CDS             complement(6365..6835)
                     /locus_tag="VGP50_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026836.1"
                     /translation="MASTRSLIDSLKRRASDMARPFIDAAQQPNATEVERAFGEAARH
                     ILRDAETRAQLLPRDEFEERVKAFKDWHRRHEAEMRREAARLGEHGFRRDERKGRITA
                     AKQRATAELDHREAEFHEWVESHALAAWIRATEDLLTLDRPSAFISTQESPARH"
     gene            7048..7710
                     /locus_tag="VGP50_05380"
     CDS             7048..7710
                     /locus_tag="VGP50_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEV8026837.1"
                     /translation="MGRGRDFRGPRRRGFNDDDFTPAEMPDFGAPRRSFGSSFNAQPA
                     MAEGPEVAATVKWFNGQKGFGFVELADGTGDAFLHVAVLQAAGRQSVLPGAKMRVATA
                     QGPKGRQVARVIDVDESTATEPPRRPREGGAGPGMARGPRDPSRDLASAVEMTGTVKW
                     FNTEKGFGFIAPEGGGKDVFIHISVLGRSGLTGLSEGQQVSMRVVETPKGREALSVTP
                     AG"
     gene            complement(7779..7985)
                     /locus_tag="VGP50_05385"
     CDS             complement(7779..7985)
                     /locus_tag="VGP50_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003625755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEV8026838.1"
                     /translation="MATGIVKWFNAQKGYGFIQPDGGGKDVFVHISAVERAGLDSLRE
                     GQKIKYEIVNDRGKDAAGNLAPLD"
     gene            8295..8510
                     /locus_tag="VGP50_05390"
     CDS             8295..8510
                     /locus_tag="VGP50_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026839.1"
                     /translation="MGSVLLVAMLSLMFFSGVLTAEMKDGDGRRIRELGVILGILMVV
                     AAYASHAIIAASWPQIASLSSLILRGS"
     gene            complement(8651..9610)
                     /locus_tag="VGP50_05395"
     CDS             complement(8651..9610)
                     /locus_tag="VGP50_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEV8026840.1"
                     /translation="MLRVAILDDYQGVALRMADWQSLHPAAQMQSFIDHVDDPDALAQ
                     RLHVFEGLVLMRERTPMPRALLERVPNLRLIVTAGMRNAAIDVAAATERRIQVCGTDM
                     LPYPTAELTWGLILALVRQIPREDRAVREGSWQTTLGLGLKGKTLGLLGLGRLGSQVA
                     KIGRAFGMDVIAWSENLTAERAAAAGVALVDKAALFARADIVSIHVVLSPRSRGIVGA
                     AEIQRMKPSAFLINTSRGPVVDEAALLEALHGRRIAGAALDVFEPEPLASDHPLLALD
                     NVIVTPHLGYVTEENYRLIYAHAVEDIRAFIDGKVLRSINKLD"
     gene            9688..>11105
                     /gene="tcuA"
                     /locus_tag="VGP50_05400"
     CDS             9688..>11105
                     /gene="tcuA"
                     /locus_tag="VGP50_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HEV8026841.1"
                     /translation="MILVYSRGMAEMACDVVVVGAGNAALAAALAAREGGARVVVLER
                     APEAERGGNSRFTAGAIRTVYDGVADLKKLIPDLTEAEIARTDFGTYTREQFYDDMAR
                     VTQYRANPDLVEMLVERSLDTLVWLRGKGVKFLPAYGRQAFKSGDKTRFWGGLTVEAS
                     GGGPGLVEALHGAARRQGIDILYGTRALALLQDERRVRGVAARQGQRRIAITAKAVVL
                     ACGGFEANTEWRTRYLGPGWDLAKVRGTRFNTGDGIRMALDAGAMPYGHWSGCHAVAW
                     DRNAPEFGDLAVGDGFQKHSYPFGIIVNARGERFVDEGADFRNYTYAKYGRVVLEQPS
                     QFAWQVFDGKVLHLLRDEYRIRQVTRARADTLEELAQKLDGVDPAGFLREIAAYNRAV
                     RRDIPFDPTVKDGRRTEGLAVAKSNWANPLDSPPFEAYQVTCGITFTFGGLRIDGDAA
                     VIDTEGEPIPGLYAAGELVGGL"
BASE COUNT         1755 a         3568 c         3967 g         1815 t
ORIGIN      
        1 gtgatcgacc aacgccgcgc cgaagcgggc ggcgagcagg cgctcggcca aggccatgcc
       61 gacggcgctg ccgaggccct gcgccaaggg gcctgtggtg gtctcgatgc cctgcgcgtg
      121 gccgcgctcg ggatggccgg cggtgcggct gccgagctgg cggaagcgct tcagctcctc
      181 aatcgtcatg ccgggatagc cggtgagatg gaggagcgcg taaagcagca tcgagccgtg
      241 gccggccgag agcacgaagc ggtcgcggtc gggccaatcg ggccgcgcgg gatcgaattt
      301 gaggaagcga ctccacagca ccgtggcgac atccgccatg cccatgggca tgccgggatg
      361 gccgctattc gcctgctcga ccgcgtccat ggcaagcgcg cggatggcat tggccatgcg
      421 gcgcagcagg gggtcgcgag gctccggaag cggcgcggcg gcggtgaggg ggcttgcggc
      481 tgtgctcatc gaggggcggg ccgcaccttg gcgggcaggg tggggcgcgt caaccgccaa
      541 ccgccattcg ccggcgccga tcacggtcaa gacctgcaac cccaagctcg cggaccgcaa
      601 gaatttcaag ttctacaacg gttagcctgc gtgtgcggcg tgtgcgtcga gcccgtttgg
      661 tgtcgaggcc tttggtcacg cctcctccgc ctccttcggt tgcgagcttc gggctttgcc
      721 actaaggtcg cgtcctctcc gatggggcgt agccaagtgg taaggcagcg gattttgatt
      781 ccgccattcc caggttcgaa tcctggcgcc ccagccagct cgagcgagtg cgaatgaaca
      841 tgaaacgaac cttttaccgg cgatctgtcg tgctggtcct gttggggctg gctgcctgcg
      901 gcatgcagac ggtccagaac gagtctctcg acacggccga aacgcaaagc ttcgatgcgt
      961 cctatcgcga tgtggtgcaa gcggctcgcg acggcatcac gacggcgggg ttcgacatca
     1021 cccaggcgga agagaaggac acgggcttcg tcctcctttt cgtgcggccg gcaagctatg
     1081 gccaattcgg cgctgtcgga cggctcgtgg tcgatcgcag cgatgctccg ccgacagccg
     1141 ttcacgtcaa ctatgagcgg cgggtcctga tctacggcgc cgggcaagaa cgcgcggcgc
     1201 gacgcatctt caagagaatg caagagacgc tgcgcgataa gggcgtgtcg cccaagtcgt
     1261 gagcggcggc cgcacggtgg tttgcgctcg acgaggaagt tccatagatc cgcgcttcac
     1321 gccgtcgaga agcagcgagg aacggccatg aagatgcaca atcccgcgac tgtcgccgcg
     1381 ccgaccggcc ctttcagcca tggcgtcgag gtgccgccca acgcgcgctg gctttgcgtc
     1441 tcgggccaga tcggcgtcgc gccggacggg agcacgcctg ccgatctcga agggcaggcg
     1501 gagcaatgct ggcgcaacgt caaggcgatc ctcgctgtgg ccggcatggg cgtggagcat
     1561 ctcctgaaat gcacgcattt cctgacgcgc gccgaggacg tcgctgccta tggcaaagtg
     1621 cgtgcgcgcc atctcggcga ggcacggccg gcctcgacgc tgctcgtcgt cgccgctttg
     1681 gcgcggccgg gattgctggt cgaggtcgag gccgtggcgg cgaaggcgta gcggaagagc
     1741 aacggtggcg cgcgcgcctc accctcccaa cgcttcgcgt tgcgcccctc cctttccggc
     1801 ggtgcgggcg agggggcttc ttgccgctcc ttagttttgc gatagggcgt cggcgagacg
     1861 gtcgtaccat tgggtttgct cggcgcggct gaaatgcgcg ttggcgccgc tcgggctcgg
     1921 cacgacgaaa agcgctgtgc cggcgatgcg cgcgctctgc aggcccagcg ccggcggcgc
     1981 gtccgcggcg agggcgcggt gaacgtgacg atagccggtc atgccgtgaa agcaggcgaa
     2041 cgacggccga tgtcgcgcga gcttggcctc gagacgcgcg atgccggcga cgaactcgtc
     2101 gtcggcgacc tcgctcgccc gcacggtcgc gcgcttgacc agatcggtga aaccgaagcc
     2161 ctgggcgagg agcgcgcggt cgtgctcggg gcggagctcc gagacgccga gagcgcggcg
     2221 cgccgcaagg ctcagggtcg agcgcgagaa acagggccag aagcgattgc tcggacgcgc
     2281 gaaataatgg ccgcgccggg cggagaagat ggacggattg atgccgacga aaagcacgcg
     2341 caagccgtcg tcgagcaggt cgggcaggtt cggtgcgccg tcatccatgg cggccgcgat
     2401 tctacctctt cgggggtggg gcgtcaggcg gctggaaggt ggtcccggcg gctccaaagg
     2461 gggcgcttcc ggccggcgcc gattggtgcg acagtgcgcc tccaaggcgg ccgggaaaga
     2521 cgccgccgct cgtcgccgcc gtccccagaa gctgcgcctg acgggaggag atgatcatgg
     2581 cggatgccgc ctacttccgc gaaaaagcgc agcaatgtcg cgagctgctc aagcacgcga
     2641 tcgagccgga agtcatcgag cagctcgaga tctgggtccg cgagttcgag gaacgcgccc
     2701 ggatcgcgca gcgtgaggcg tcgagggact gagcggccgg cggttggtca gcggaaatta
     2761 ctccttcgcg cgggggcttt cgcggttgca agagcaaccg ctggcgcctc tcgcgacgac
     2821 gcttcgcgtc gcgcgggtcg cgcgggcttg accgggctgc gccttgttga atgataggca
     2881 aaccatgccg actcagcgcg ccgccttgtt cgtcctcacc acgctcctcg tggtgctggg
     2941 cttatggatc ctgcggcatt tcctgccggc gctcgcctgg gcgggggtgc tggccatcgg
     3001 cacctggccg ctctacagcc gctttcgccg gctgctgccg ccgcggctcc aaccctcgac
     3061 ctgggcgccg ctcggcttca cgctgctggt cggcgtcgtc ttcgtcctgc cgctgacgct
     3121 cttcgccatc gaggtgggaa gggaggcgct ggtcctggtg catcaagtgg gggccatcga
     3181 gcaaaccggg atgccgccgc ccgactggct cgcgcatctg cccttcggca gctacgccac
     3241 ggcgtggtgg cagaacaatc tgagcgaccc gcaagcggcg caggagctga tggggcgggt
     3301 tgaccggagc gtggtcctcg aatggctgcg ctcgctcggg ctccagctct tcgcgcgctc
     3361 gacgatcctg gtcttcacgc tgctcgcgct cttcttcctc tatcgcgacg gattgaccct
     3421 cgcgcatcaa ctggcggcgc tcgcggcgcg caccttcggg ccgacggggg aggacctcgg
     3481 cgatcacgtc atagccgcgg tgcgcggcac ggtcgacggg ctggtgctgg tcgggttggg
     3541 ggaagggctg ctgctcggcg ccggctatgc cgtcctgggc ctgccgcacg cgctgctgct
     3601 gggcgcgatt acttgcgtgc tggcgatgat cccgttcggg gcgccggtga tcttcggtct
     3661 cggcagcctc ttcctgctcg cgcaatcagg cgtcgccgcg gcggtgggcc tcgccgccta
     3721 cggcgtcgtc atcagcttca tcgccgacca tttcgtgcgg ccggtgctga tcggcggcgc
     3781 ggtgcgactg ccgtttctct gggtgctgct cggcatcctc ggcggcgtcg aaagcttcgg
     3841 gctcttggga ttgttcctcg ggcccgccgt catggcggcg ctcatcgcgc tctggcgcga
     3901 atggacggcg catgcgggcg gagaaggaaa agaagattca ccaatgagac cgtgagccaa
     3961 tgagaagaag aaagatggag caaagagaca cagagccaca gagcgcgcgc cgagcgatct
     4021 cttcctctgt gtctcggtgt ctctgtggtg aaatcgccgc tgcggcggtt cggtctctca
     4081 ccgcctcacc gcctttgtgg tcaatcttct agattaggga tcgatcagga cgccgggatt
     4141 gaggaggcct tgcgggtcga gcgcgacttt ggcggctttc agcgcggcgg cgaagaggtc
     4201 ggggcgctgg cggtcgtacc aggggcgatg atcgcggccg atggcgtggt ggtgcgtgat
     4261 ggtgccgccg ccggcgatga gcgcgtcgga ggcggccgtc ttgatcgcct gccattgctc
     4321 gatgaggtgg ccgtggcggc cgaggccgtg gaaggagaaa tacggtgcgg ggccgtccgg
     4381 atagacatga gtgaaacgac aggtgacctg gcccggccgg cccgtcgctg cacggatcgc
     4441 ctgctcggtc gcgctcttga ccttgtcgtg aaacacctcg aagcgatccc aggtgattgc
     4501 cgtctcgaaa gtgtcgctga tgatggcgcg cgggaccatg cgctcgcgcg catagggcat
     4561 gcggataaag gcgttgcgcc agacgcccgc agcaccgtcg cgatgcgctt cggcgccact
     4621 cgtatcttca ggtgtcccgc cgtgatcggc gcagcattcg agcgcgcgct tcatccaggc
     4681 atcgacggga tggtcgccgg attcgaaagc caggaccatg atggcgacgc ggccgtcgcc
     4741 ggcgccggtg ttgtaggcct cttggggatc gaggatgcgg caattcgcgg gatagaggcc
     4801 ggcctgggcg atcatgcgca ccgcctgggc ggcggtgaac aaatccttga agcctacggc
     4861 gccgccggca cgaaagcgcg gccgatcctg aagccgcatc caggcttcgg tgatgacgcc
     4921 gagcgtccct tcggaaccga tgaacagacg atcggggctc gggcccgcgc cggagcccgg
     4981 cagccgccgc gtctcgacga cgcccctggg cgtcaccacg cgcaggctct cgacgagatc
     5041 gtcgatatgc gtcaggaggg tcgcgaaatg accgccggag cgggtggcga tccagccgcc
     5101 cagggtcgaa tactcgaagc tctggggaaa gtggcgcagc gtcagctcat ggggcttgag
     5161 ctgggcctcg agcgcagggc cgaacacacc gccctcgatg cgggccgccc gcgaggcgcg
     5221 gtcgacctcg cgcacgcgat ccatgcggcc gagatcgagc gtgagcgcgg ccttgtagcg
     5281 gccgccgtcg agccggggct cgacgccgcc cacgacgctc gagccgccgc cgaagggcac
     5341 gatcgcggcg cgggcgccgc ccgcccagtc gagcacggcc gcgacctcgg cctcgttgcg
     5401 cggataggcc acgacatcgg gcgcgttggc atagtcgccg gcgaggccgc gcacgtaatc
     5461 ggggaaagac ttgccgtagg catggacggc gcggtcatgg gtcgcgctcg agcagaaagc
     5521 agcgagcgcc ggcggcggcg agaggcgcgg cggccgcaag gcgagatcgg gaagacgcgg
     5581 cggcgggcgc tcgtcgaagc gagtgacgcc gaagagcttc cgatagcgct cgaggccgaa
     5641 agcgtcttcc gccgcggtca ggccttcgcc ttcgaggccc caaccgaaga atttgcggcg
     5701 cttcgccgtc atggcaaccc gctccgtacc gacggtcgaa agatctctga tccgcgcatc
     5761 ctcccaccgc accgcgatgg gcccctccct tcccgacgag ggagaggggg acggctcagc
     5821 ggcggagccg actctcgatg agctcgcgca ctttgcgctg ctcccaatcc tgggtcgtcc
     5881 aggggaggag ctcatgggcg tgcagcccgt gcagcagcac gagcatgccc caaccgatga
     5941 gcggcgcgta gaaccaggcg ggcccgggcg tcgtgctgtc gatgaggaac aggacgaagt
     6001 tgatgatcac ataagcggtc agatggctgt agaagccgcg gagcgagcgg acgcgacgcc
     6061 atgcccattg ctcgatctcg gcgacgtgcg ccgcctcgcg cgccccaatc cccggcctca
     6121 attcaatcgg ttcactgcct tgcccacgca tggccgcatc cacttcccaa cccccgatcc
     6181 gccgctctgg acccgcgccc actctatcga gaatcgccgg cgaaatacag ggcaagcgcg
     6241 gtgacggcgc agttatctca gcagaactta tcgagttgaa atgaagcgct cgacttcatt
     6301 ctccggtgtc ggttgtgact ctccgttcgg ccctgcggcg ccgtgcggga gacggttttt
     6361 tcgctcagtg ccgcgccggg ctttcctgcg tcgagatgaa ggcggagggg cggtcgaggg
     6421 tcaggagatc ctcggtggcg cggatccagg ccgcgagcgc atggctctcg acccattcat
     6481 ggaactcggc ctcgcggtgg tcgagctccg cggtcgcgcg ctgcttggcg gcggtgatcc
     6541 gccctttgcg ctcgtcgcgg cgaaagccat gctcgccgag gcgcgccgcc tcgcggcgca
     6601 tctccgcttc atggcggcga tgccagtcct tgaaggcctt gacgcgctcc tcgaactcgt
     6661 cgcgcggcag gagctgggcg cgcgtctcgg cgtcgcgcag gatgtgccgc gccgcctcgc
     6721 cgaaggcgcg ctcgacctcg gtggcgttgg gctgctgggc ggcgtcgatg aaggggcggg
     6781 ccatgtcgct ggcccggcgc ttcaagctgt cgatcaggga tcgggtggag gccatgacac
     6841 actccggcgg gctctgccga cgcattgggc aaggctaaac ccggagattc gccgctgtcc
     6901 agtcgggtcg ggcggcggat cgcgggcgaa gccggaaagg cgagacgatg cgataatacg
     6961 ccgcggaaat ttcacggcga tgtgctaagt tgggcgttcc ccaggggcgc aagcgtccat
     7021 cacagcgagc gaaggcgagc aagacttatg ggtagaggca gggatttccg cggcccgaga
     7081 cgacgcggct tcaatgacga cgatttcaca ccggcggaga tgcccgattt tggcgcaccg
     7141 cggcggtcct ttggctcctc cttcaatgcc cagccggcca tggcggaagg accggaggtc
     7201 gccgccaccg tcaaatggtt caacgggcag aaggggttcg gcttcgtcga gctggccgac
     7261 ggcacggggg atgcgttcct ccatgtcgcc gtgctccagg cggcgggacg ccaatccgtg
     7321 ctgcccggcg ccaagatgcg cgtcgccacg gcgcaaggcc ccaaaggccg gcaggtggcg
     7381 cgcgtcatcg acgtggacga gagcacggcc accgagccgc cgcgccgccc gcgcgaagga
     7441 ggcgccggcc ccggaatggc acggggcccc cgcgatccgt cgcgggacct cgcgagcgcc
     7501 gtcgagatga ccggcaccgt caaatggttc aataccgaga aagggttcgg cttcattgcg
     7561 cccgagggcg gtggcaaaga tgtgttcatt cacatctccg tcctgggacg ctcgggcctc
     7621 accggcctct cggaaggaca gcaggtgtcg atgcgggtcg tcgagacgcc caaagggcgc
     7681 gaagcgctgt cagtgacgcc ggccggctaa gggtcgacgc ggccaaaaga aaacgggcgg
     7741 cgccaaaagc gccgcccgtt tctcggatca agtcgtgatc aatccagcgg cgcgaggttg
     7801 ccggcggcat ccttgccgcg gtcgttgacg atctcgtatt tgatcttctg accctcacgc
     7861 agcgagtcga ggccggcacg ctcgaccgcg gaaatatgga cgaacacgtc tttgccgccg
     7921 ccgtcgggct ggatgaagcc atagcccttc tgcgcgttga accatttcac gataccggta
     7981 gccaaggcat agtctccttt agaagtggtg gaccggcacg ctgcccttcg tgcagcatgc
     8041 cgcgcagttc tcggcctctt ggggagcttg gagcaacgcc cggcgggcga tcggcaaggc
     8101 cgccgaagct gaaactggcc gccccaccat ggcacttctc cccagccgac acaatcgcca
     8161 acgcctaaac tgccgaatac cgaggcccaa gcctcaactt cttgggggtg ctgtgacgag
     8221 ctacccctgg ccatggattc tgctagattg acgcccaaga cccggaaggg gccgggcgcg
     8281 gtcgggggag acgcatgggt tcggtactgt tggtcgccat gttgagtttg atgttctttt
     8341 cgggcgtgct gacggccgag atgaaagacg gggacggccg acggattcgc gagctcggcg
     8401 tgatcctcgg cattctgatg gtggtggcgg cctatgcgag ccatgccatc attgcggcga
     8461 gctggccgca gatcgcgagc ctctcttctc tgatcctgcg aggctcgtaa ggcgccgttc
     8521 gcctttcaat ttgccgtgag gccgcatgcc ggggtgattt accgcgacat aggccggcac
     8581 gcttgatcga ctctcgtccg acgctcgcgc acagcccttc gtcgaaggcg gcgatggccg
     8641 tgctcggcac tcagtcgagc ttgttgatgc tgcgcagcac tttgccgtcg atgaaggcac
     8701 ggatgtcctc gacggcgtgg gcgtagatga ggcggtagtt ctcctcggtc acatagccca
     8761 gatgcggcgt gacgatgacg ttgtcgagcg ccagcaaggg atggtcgctg gctaagggtt
     8821 cgggctcgaa gacgtcgagc gcggcgccgg cgatgcgccg gccgtgcagc gcctcgagga
     8881 gcgccgcctc gtcgacgacc ggtccgcgcg atgtgttgat gagaaaggcg ctcggcttca
     8941 tgcgctggat ctcggcggca ccgacgatgc cgcgcgagcg cgggctcaag acgacatgga
     9001 tgctgacgat gtcggcgcgc gcgaagagcg ccgccttgtc gacgagcgcg acgcccgccg
     9061 cggcggcgcg ctcggcggtg aggttctcgc tccaggcgat gacgtccatc ccgaaggccc
     9121 gtccgatctt cgcgacctgg ctgccgagcc ggccgaggcc gaggagcccc agcgtcttcc
     9181 ccttgaggcc gagccccagc gtcgtctgcc aggatccctc gcgcacggcg cggtcctcgc
     9241 gcggaatctg acggacaagc gccaggatca gcccccaggt gagctccgcc gtcggataag
     9301 ggagcatgtc ggtgccgcag acctggatgc gccgctcggt ggcggcggcg acatcgatcg
     9361 cggcattgcg catgccggcg gtgacgatga ggcgcagatt cgggacgcgc tcgaggaggg
     9421 cgcgcggcat cggcgtgcgc tcgcgcatca gcacgagacc ttcgaagaca tggaggcgct
     9481 gggcgagcgc gtcggggtcg tcgacgtggt cgatgaagga ttgcatctgc gcggccggat
     9541 gcaggctttg ccaatcggcc atgcgcaagg ccacgccttg ataatcgtcg aggatcgcca
     9601 cccgcagcat tcgcctctcc catcgtgctc ataagccctc tcccgcgatg cgccgcggtg
     9661 cgggagaggg attggtatgg cgcctccatg atccttgtct atagtcgagg catggcggag
     9721 atggcgtgcg acgtcgtggt ggtgggggcg gggaatgcgg cgctggcggc ggcgctggcg
     9781 gcgcgcgagg ggggcgcccg ggtggtggtg ctggagcgcg cgcccgaagc ggagcgcggc
     9841 ggcaacagcc gcttcacggc aggcgccatc cgcacggtct atgacggcgt cgcggatctg
     9901 aagaagctca ttcccgacct caccgaggcg gagatcgcgc gcaccgattt cggcacttac
     9961 acccgcgagc agttctacga cgacatggcg cgcgtcacgc aatatcgcgc caatccggac
    10021 ctggtcgaga tgctcgtgga gcggagcctc gacacgctgg tctggctgcg cggcaagggc
    10081 gtgaagttcc tccccgccta cggccggcag gccttcaaga gcggcgacaa gacgcggttc
    10141 tggggcgggc tcacggtcga ggcgtcgggc ggcgggccgg gtctcgtcga ggcgctccat
    10201 ggcgcggcgc ggcgccaggg catcgacatc ctctatggca cgcgggcgct ggcgctgctc
    10261 caggacgagc gccgcgtgcg cggtgtcgcg gcgcggcagg ggcagcgccg catcgccatc
    10321 acggccaaag cggtggtgct cgcctgcggc ggcttcgagg ccaataccga atggcgcacg
    10381 cgctatctcg ggcccggctg ggacctcgcc aaggtgcgcg gtacgcgctt caataccggc
    10441 gacggcatcc gcatggcgct cgacgcaggc gcgatgccct atgggcattg gtccggctgc
    10501 catgccgtcg cctgggaccg caatgcgccg gaattcggcg acctcgcggt gggcgacggc
    10561 ttccagaagc attcctatcc cttcggcatc atcgtcaatg ccaggggtga gcgcttcgtc
    10621 gacgaaggcg ccgatttccg caactacacc tatgcgaaat acggccgcgt cgtgctggag
    10681 cagccgagcc agttcgcgtg gcaggtgttc gatggcaagg tgcttcatct gctgcgcgac
    10741 gaatatcgca tccgccaggt gacgcgggcc cgcgccgaca cgctcgagga gctcgcgcaa
    10801 aagctcgacg gcgtcgatcc cgcgggattc ctgcgcgaga tcgccgccta taaccgtgcc
    10861 gtccgccgcg acattccctt cgacccgacg gtgaaggacg ggcgccgcac cgaaggcctc
    10921 gccgtggcga aatcgaactg ggccaatccg ctcgactcgc cgcccttcga ggcctatcag
    10981 gtgacctgcg gcatcacctt caccttcggc ggcttgcgca tcgacggcga tgccgcggtg
    11041 atcgacaccg agggcgagcc gatccccggc ctctatgccg cgggcgagct ggtgggcggc
    11101 ctctt
//
LOCUS       DASYNS010000171         7611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2379408, whole genome shotgun sequence.
ACCESSION   DASYNS010000171 DASYNS010000000
VERSION     DASYNS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379408"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VGP50_05405"
     CDS             <1..684
                     /locus_tag="VGP50_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV8026842.1"
                     /translation="LAVHIGSQLTELTPFELAFARVGELIRAARRDGIALGRLDLGGG
                     LGNRYRHETVPSIEDYARLVKRVTDGLEVALAIEPGRRIVANAGILAARILYVKEGAA
                     RRFLVVDAAMNDLIRPSLYGAWHEIIPILAPAAGAARRPIDVVGPVCESGDSFAEQRL
                     LPPLAAGDLVALLSAGAYGAVMSSSYNTRLMVPEVLVRGDAFAVIRPRPGYDELLSQD
                     KVPEWLAWR"
     gene            703..3219
                     /locus_tag="VGP50_05410"
     CDS             703..3219
                     /locus_tag="VGP50_05410"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HEV8026843.1"
                     /translation="MGETKESPAQTVNAPRMALRLRLAAAALAWERLWPALWPATAII
                     GVFLALALLDLLPQLPGLVHAASLALLVGLLGLAIVTALRRFRLPDRDAGRRRIEQAS
                     GLAHRPLTALEDRLTVGSDDAATTALWQAHRQRMAAQARRLTVGWPAAGLVRRDPMAL
                     RAALVLVLLIAVVDAGRDWPQRLLRAVTPSFALGGPPAMVALDIWMTPPDYTGLPPQF
                     LSAGAVDAAVAVPTGSTVLAQVHGGRGLPRLRVDDATTEFAQIDDSNFKGSVPLTAGQ
                     RLAVEQDGRTLGAWPITIVPDLPPKIEFVKPPQRTQRGTLRFEYQASDDYGVESAKAV
                     ITRPNDPTGDWIALDLALPGQHLKEAHDTTYHDLTAHPWAGLPVDIRLEAADALGQTG
                     NSDMLRITLPERVFHHPVARAIIEQRKQLTLDPSQRDVVAETLSDLSARPRLYNDDKV
                     VFLALRTAQARLVLDQSATIIPSVQQLLWDTAVRIEDGRTSLVQRDLRDAMKALQDAL
                     ARNASDAEIDHLMHELQQAIDHYLQALAENMQQQQGNEDQQPLDPSRMLSAQDLQNML
                     NRARELARTGSRDAARDMLSQLQEMLENLRLARPGEMRGGQGQAMRQMQEMMRRQQQL
                     LDKSFRQSHQGQQRGQQGQNGQQGQNGQNGQAGQNGQPRAGGQGDGDMAGDQESLRRQ
                     LGEMMRQLGEQTGDIPQSLGRAERAMHGAADALSQGQPGNAIAPQTEALDQLQQAARS
                     MAEQMMGKNGDGSPDGQEPGDRSGMSQTDRDPFGRLNAQDHADGGVDEGGRMRMGEGP
                     NSNYALEKARGILEELRRRAGERSRPELERDYIDRLLKQF"
     gene            complement(3584..4258)
                     /locus_tag="VGP50_05415"
     CDS             complement(3584..4258)
                     /locus_tag="VGP50_05415"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HEV8026844.1"
                     /translation="MIVTCPVCSTRYVVDPRAISATGRLVRCASCAHTWHQLPQEDEA
                     PRRIELTAPEPVIEHAPSGRVQLPALPRRQSRLPALLWTLYFLVLLALIGGGLWWARD
                     QVVAYWPPAAQYYDLLGVPAPPAPSELEIQKLATSRDVQDGLETLVIQGEVVNVSKAP
                     RKLPKLKVILQDGEKHELQSWSFDVTDDQVPPGGVVPFRTSVSQPSAAVRGVVVTLDT
                     HALSGS"
     gene            4398..5078
                     /gene="ftsE"
                     /locus_tag="VGP50_05420"
     CDS             4398..5078
                     /gene="ftsE"
                     /locus_tag="VGP50_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV8026845.1"
                     /translation="MIRFENVGLRYGAGPEVLHDITVELPPGSFHFLTGSSGAGKTSF
                     LKLIYLAERPTRGLVSLFGRDIATTPRQDLPALRRKIGVVFQDFRLIEHLSAFDNVAV
                     PLRVAGIAEAQIREHVGELLRWVGLAEKLEARPATLSGGQQQRLAIARAVIGRPTLLV
                     ADEPTGNVDDEMALRLMRLFEEMNKLGTTLVIATHNEHLAKRFGHARLHLDRGELTIE
                     SNRERQVL"
     gene            5078..5974
                     /locus_tag="VGP50_05425"
     CDS             5078..5974
                     /locus_tag="VGP50_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HEV8026846.1"
                     /translation="MLRHRLDIPLDNDGSVRFLPWLIALMVYLAALALAGTLVLNTTL
                     ARYDRTLSGTLTVQLPAASADGRDDGGLAATLGALRRVPGVTSADPLGRDATARLVEP
                     WLGTSFAPEELALPQLIDVRVDPERPPDLAALAATLAAAAPAAVLDDNREWLDRLATP
                     MRAVEVVTLAIVAFIGMVAALTVVFTTRAGLSVHHTVIEVLHLIGARDGYIAAQFQGH
                     ALRLGLAGGVFGLLLAVATLFGIAQAAARTALLGGRVQLLPALGLAPWHWAVLALLPL
                     AAGIIAMITARVTVLRALARMP"
     gene            5971..6600
                     /locus_tag="VGP50_05430"
     CDS             5971..6600
                     /locus_tag="VGP50_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEV8026847.1"
                     /translation="MSGSRAPRRLSLPAVLILALLVAWFGGFVWFLRAIPDEVSDPET
                     ATDAIVALTGGSLRVQSGLALLAAGKARKLFVSGVHPGTDVAALMRAEHQSSGATLAC
                     CVVLGHEADNTLGNAEETALWMHREGFHSLRLVTASYHMPRSLLEFARAMPEVRIVAH
                     PVFPEHVLQGYWWARPRTAMLIFAEYQKYLLALARPVMFGRPDTASEPS"
     gene            6597..7376
                     /locus_tag="VGP50_05435"
     CDS             6597..7376
                     /locus_tag="VGP50_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV8026848.1"
                     /translation="MILLRSLLFNLGFYLMTAAMALLGLPVLLLPERLGMAFGRSWAA
                     LVLKLLGWCCGLTHELRGAENLPPGPVIIAMKHQSAWDTLAVPVIFGNPAIVLKRELM
                     MIPFYGWYLWKAGMIAIDRKAGAVALRRLVAAGARAAALRRPIVIFPEGTRTAPGTHH
                     PYQPGVAALYRQLELPLVPVAVNSGLYWGRRRFVKRPGRIIMEILPAIPPGGERRAVM
                     GELEQRIEAATTRLLAEAQAAGATGEKAVDDSTERPGGTRG"
BASE COUNT         1162 a         2652 c         2553 g         1244 t
ORIGIN      
        1 ctcgccgtgc atatcggctc gcagctcacg gagctgacgc ctttcgagct ggccttcgcg
       61 cgggttggcg agttgatccg tgcggcgcgc cgcgacggca tcgcccttgg ccgcctcgac
      121 ctcggcggcg ggctcggcaa ccgctaccgg cacgaaaccg tgccgtcgat cgaggactac
      181 gctcgcctag tcaagcgggt gacggatgga ctcgaagtcg ctctcgccat cgagccggga
      241 cgccggatcg tcgccaatgc gggtattctc gccgcgcgca tcctctatgt gaaggagggc
      301 gccgcacggc gcttcctcgt ggtcgatgct gcgatgaacg acctgatccg gccctctctc
      361 tatggtgcgt ggcacgagat cattccgata ctcgctcccg ctgcgggcgc ggcgcgccgt
      421 cccatcgacg tggtggggcc ggtctgcgag tcgggcgaca gcttcgccga gcaacggctg
      481 ctgccgccgc tggcggcggg cgatttggta gcgctgctct ccgctggagc ctatggcgcg
      541 gtaatgagtt cgagctacaa tacccggctc atggtgccgg aggtgctggt gcgcggcgat
      601 gccttcgccg tcatccgccc gcggcccggc tatgacgagc tcttgtccca ggacaaggtg
      661 ccggagtggc tcgcctggcg ttgacggcga gaggtggtga cgatgggcga gaccaaggaa
      721 tcgccggccc agacggttaa cgcgccccgc atggcgctgc gcttacggct tgccgccgct
      781 gcgcttgcct gggagcggct atggccggcg ctttggcccg cgaccgccat tatcggcgtc
      841 tttctcgccc ttgccctcct cgacctcctg ccgcagctgc cgggcttggt gcatgcggca
      901 tcgctggccc tcctcgtcgg gctcctcggc ctcgccatcg tcacggcgct gcgccgcttt
      961 cgccttcctg atcgcgatgc cgggcgccgg cggatcgagc aggcgagcgg cctcgcgcac
     1021 cggccgctca ccgcgcttga ggaccggctg accgtcggta gcgatgatgc cgccacgacg
     1081 gcgctgtggc aggcgcatcg gcaaaggatg gccgcacagg cgcgccgcct caccgtcggc
     1141 tggccggcgg ccggactcgt tcgccgcgat cccatggcgc tccgcgccgc cctggtgctg
     1201 gtgctgctga tcgcggtggt cgacgccggc cgcgactggc cgcagcggtt gttgcgggcg
     1261 gtgacgccga gcttcgcgct gggcgggccg ccggcgatgg tcgcgctcga catctggatg
     1321 acgccgcccg actacacggg actgccgccg caattcctgt cggcaggcgc cgtggacgcg
     1381 gcagtcgccg tgccgaccgg cagcaccgtc ctggcgcagg ttcatggcgg ccgcggtctg
     1441 ccgcggctca gggtcgatga cgccacgacc gagttcgccc agatcgacga cagcaatttc
     1501 aaaggcagcg tcccgctcac ggccggccag cgcctcgccg tcgagcagga tgggcgcacg
     1561 ctcggcgcct ggccgatcac catcgtcccc gacctgccgc cgaagatcga gttcgtcaag
     1621 ccgccgcagc gcacccagcg cggcacgctg cgcttcgagt accaggcaag cgacgactac
     1681 ggcgtcgaaa gcgcgaaagc ggtcatcacg cgccccaacg acccgaccgg tgactggatc
     1741 gcactcgact tggcgctgcc cgggcagcac ctcaaggaag cgcatgacac gacctatcac
     1801 gacctcaccg ctcatccctg ggcgggcctg ccggtcgata tccgcctcga ggcggcggat
     1861 gcgctgggcc agacgggcaa cagcgacatg ttgcgcatca ccttgcccga acgcgtcttt
     1921 caccaccccg tggcccgcgc catcatcgag cagcgaaagc agctgactct cgatccctcg
     1981 cagcgggatg tcgtcgccga aacgctctcc gatctctcgg cgcggccgcg cctctacaac
     2041 gacgacaagg tggtgttcct ggcactgcga accgcccagg cgcggctcgt cctcgaccaa
     2101 agcgctacca ttattccctc ggtccagcag ctgctgtggg acacggccgt ccgcatcgag
     2161 gacggccgga catcgctcgt ccagcgcgac ctgcgcgatg ccatgaaggc gctgcaggat
     2221 gcgctggcgc gcaacgcgtc cgacgccgag atcgaccatc tcatgcacga gttgcagcag
     2281 gcgatcgacc actaccttca ggcgctcgcc gagaacatgc aacagcagca gggaaacgag
     2341 gatcagcagc cgctcgaccc gtcacgcatg ctgagcgccc aggacctcca gaacatgctc
     2401 aaccgcgcgc gcgagcttgc acgcacggga tcgcgcgatg ccgcgcgcga catgctgtcg
     2461 cagctccagg agatgcttga gaatctgcgc ctggcgcgtc ccggcgagat gcgcggcggc
     2521 cagggccagg cgatgcggca gatgcaggag atgatgcggc gccagcagca gctcctcgac
     2581 aagagcttcc gccagtcaca ccaaggccag cagcgcgggc agcagggcca gaatgggcag
     2641 cagggccaga atgggcaaaa tggtcaggcc gggcaaaacg gccagccgcg cgccggcggt
     2701 cagggcgacg gcgacatggc cggcgaccag gagtcgctgc ggcgccagct cggcgagatg
     2761 atgcgccagc tcggcgagca aacgggtgac atcccacagt cgctgggccg cgccgagcgc
     2821 gcgatgcacg gtgccgccga tgcgctctcg caagggcagc ccggcaatgc gatcgcgccg
     2881 cagaccgaag cgctcgatca gcttcagcag gcggcgcgca gcatggccga gcagatgatg
     2941 ggcaagaacg gcgatggctc gccagacggt caggagcctg gcgaccgcag cggcatgagc
     3001 cagacggacc gcgatccttt cggccggctc aacgcgcagg atcatgccga tggcggcgtc
     3061 gacgagggcg ggcgtatgcg catgggcgaa ggcccgaaca gcaattacgc tctggaaaag
     3121 gcgcgcggca tcctcgagga gctgcgccgc cgcgccggcg aacgcagccg ccccgagctc
     3181 gagcgcgact acatcgaccg cctgctgaag cagttctgag ctctcggccg caccgattac
     3241 ggcaacccga gccgagggcg gtcggctcga agcggcggtg ccgtggcaga gtgcctacaa
     3301 gctgaggtga taggcgatcc ccagccggaa gacatgttgg tcgacgggca cgttggttcg
     3361 gacattgaag ctcggtccag gcggcggatt gaagtactcc gcatcctgca gatgaatgtt
     3421 cgcgctgccc gtgcctcact tgcccttcag ccaaggcgcg acgcggatcg agctttcgag
     3481 cagcatgcgc aaggccgcga cgaaggccag cggctggtcg agcatcagat ggtgctgcga
     3541 cgcgtcatcc tcggggacga gaatgcgcgc cttgcttctc gcctcaggac cccgagagcg
     3601 cgtgtgtgtc gagcgtgacg acgacgcccc tcaccgccgc gctgggctgc gagacgctgg
     3661 tgcggaaggg tacgaccccg ccgggcggca cctggtcgtc cgtgacgtcg aaggaccagg
     3721 attgcagctc gtgcttctcg ccgtcctgca ggatgacctt gagcttgggc agcttgcgcg
     3781 gagccttgga gacgttcacc acttcgccct ggatgaccaa cgtctcgagg ccgtcctgca
     3841 catcgcgact cgtcgcgagt ttctgaattt cgagctcgct cggcgctggc ggggcgggca
     3901 cgccgagcag gtcgtagtac tgcgccgcgg gcggccaata ggcgaccacc tggtcgcgcg
     3961 cccaccacag cccgccgccg atgagcgcga gcagcaccag gaaatagagc gtccacagca
     4021 gcgccggcag ccggctctgg cgtctcggca gcgccgggag ctgcacgcgg cccgagggcg
     4081 catgctcgat cacgggctcg ggcgcggtga gctcgatgcg acgcggtgcc tcgtcctcct
     4141 gcggcagctg gtgccaagtg tgcgcgcagc tggcgcaacg caccagcctt ccggtggcgc
     4201 tgatcgcgcg cggatcgacg acatagcggg tggagcacac cggacaggtt acgatcatcg
     4261 agtccctgcg cctttgttct gcccttatag gggtcgaaaa tggccgaggc aagaaagccg
     4321 ccggctttgg gacgcgtctg gacgccggcc ggcgcatcgt gccatggtgc gccaagcggg
     4381 gacggagagc gcgctccttg attcgctttg agaatgtcgg tttgcgttat ggcgccgggc
     4441 ccgaggtttt gcacgatatc acagtcgagc tgccgcccgg ctccttccat ttcctcaccg
     4501 ggtcgagcgg tgcgggcaag acgagcttcc tcaaactcat ttatctggcc gagcgcccga
     4561 ctcgcggtct tgtttcgctc ttcggccgcg acatcgccac cacgcctcgc caggacctgc
     4621 cagcgctccg gcgcaagatc ggcgtcgtgt ttcaggattt ccgcctcatc gagcacctct
     4681 cggccttcga caacgtcgcg gtgccgcttc gcgttgccgg catcgccgag gcgcagatcc
     4741 gcgagcatgt gggcgagctc ctgcgctggg tcgggctcgc cgagaagctc gaggccaggc
     4801 ccgcgacact ctcgggcggt cagcaacagc gtctcgcgat cgcccgcgcc gtgatcggcc
     4861 ggccgacact cctggttgcc gacgagccga ccggcaacgt cgatgacgag atggcgctcc
     4921 ggctgatgcg gctcttcgag gagatgaata agctcggcac gacgctggtg atcgccacgc
     4981 ataacgagca tctggcgaag cgcttcggcc atgcgcgact ccatctcgac cgcggcgagc
     5041 tcaccatcga gagcaaccgc gagcggcaag tgctctgatg ttgcggcacc gcctcgacat
     5101 accgctcgac aatgatggct cggtgcgttt tctcccctgg ctgatcgcgc tcatggtcta
     5161 tctcgccgcg ctcgcgcttg ccgggacact cgtgctcaac accaccctcg cgcgctacga
     5221 ccgcaccctc agcggcacgt tgacggtgca gctcccggcg gcatcggccg acggcaggga
     5281 cgatggcggc ctcgccgcga cactcggggc gctgcgccgc gtgccgggcg tcaccagtgc
     5341 cgacccgctc ggtcgcgacg ccaccgcgcg tctcgtcgag ccatggctcg gcacctcctt
     5401 cgcgcccgag gaactggcgc tcccgcagct catcgatgtg cgcgtcgacc ccgagcggcc
     5461 gccggatctc gcggcgctgg cggcgacgct cgccgccgcg gcgcccgccg ccgtgctcga
     5521 cgacaaccgc gagtggctcg accgcctggc gacgccaatg cgggcggtcg aggtcgtcac
     5581 cctcgccatt gtcgccttca tcggcatggt ggcagcgctc accgtcgttt tcacgacgcg
     5641 cgccggcctc tcggtgcatc acacggtgat cgaggtgctg cacttgatcg gcgcccgcga
     5701 cggctatatc gccgcgcagt tccagggcca tgcgctgcgc cttggactgg cgggcggcgt
     5761 cttcggactg ctactcgcgg tcgcaacgct cttcggcatc gcccaggccg ccgcgcgcac
     5821 cgcgctgctc ggcggccgcg tgcagcttct gccggcactg ggcctcgccc cctggcattg
     5881 ggcggtgctg gcgctgctgc cgctcgcggc cggcatcatc gccatgatca cggcgcgcgt
     5941 caccgtgctg cgcgcgctgg cgcggatgcc atgagcggca gccgcgcgcc gcggcggctt
     6001 tcccttcccg ccgtcctgat cctcgccctt ctcgtggcgt ggttcggcgg tttcgtctgg
     6061 ttcctgcgcg ccattcccga tgaagtgagc gatcccgaga cggccaccga tgccatcgtc
     6121 gcgctcaccg gcggcagcct gcgcgtgcag agcgggctcg ccttgctggc ggcaggcaag
     6181 gccaggaagc tcttcgtatc gggtgtccac cccggcaccg acgttgctgc gctgatgcgc
     6241 gccgagcacc agtcgagcgg cgccacactc gcttgctgcg tcgtcctcgg ccatgaggcc
     6301 gacaatacgc tcggcaacgc cgaggagacg gcgctctgga tgcaccgcga agggtttcat
     6361 tcgctgcgcc tcgtcaccgc cagctatcac atgccgcgca gccttctcga attcgcgcgc
     6421 gccatgccgg aagttcggat cgtcgctcat ccggtctttc ccgagcatgt tctgcaaggc
     6481 tattggtggg cgcggccgcg gacggcgatg ttgattttcg ccgaatatca gaaatacctg
     6541 ctggccttgg cgcggccggt gatgttcggg cggcccgata cggcgagcga gccgtcgtga
     6601 tcctgctccg ctctctgctc ttcaacctcg gcttctatct gatgacggcg gcgatggcct
     6661 tgctcggcct ccctgtgctg ctcctgccgg agcgcttagg gatggccttc gggcgatcct
     6721 gggccgcgct ggtgcttaag cttctcggct ggtgctgcgg cctcacccat gagctgcgcg
     6781 gtgccgagaa tctgccgccc ggtcccgtca tcatcgcgat gaaacaccag tccgcctggg
     6841 atacgctcgc ggtaccggtg atcttcggca accccgccat cgtgctgaag cgcgagctga
     6901 tgatgatccc tttctatggc tggtatctct ggaaggccgg catgatcgcc atcgatcgca
     6961 aggcgggcgc cgtcgcgttg cgccgcttgg tcgcggcggg cgcgcgcgcc gcggcgctcc
     7021 gccgtcccat cgtgatcttc cccgaaggca cccgcaccgc gcccgggacg catcatccct
     7081 accagcccgg ggttgctgcg ctctatcgcc agctcgaact acccctggtc ccggtcgccg
     7141 tcaattccgg cctctactgg gggcgccgcc gtttcgtgaa gcggcctggc cggatcatca
     7201 tggagatcct cccggcgatc ccgcctggtg gcgagcgccg ggccgtgatg ggcgagctcg
     7261 agcagcggat cgaggccgcg accacgcgat tgctggccga ggcccaagcg gctggcgcga
     7321 cgggtgaaaa ggctgtggat gactcgacgg aacgtcccgg tggaacccgg ggataacttg
     7381 aatattcctt ctggaataat atgttagtgg cgtgaacaga atgctatcag tgccgcaccg
     7441 ctgtgggaaa caaacaagga acctttcctt gaagccggcc tgagcggccc gtaaactcta
     7501 gcgggctccc gccgtcgcgg gacgccctgg agactcaccc gattccgacc gtcgcctcga
     7561 tctcccagca gatctgggac atgaaatacc ggctgaaagg ccccgacggc a
//
LOCUS       DASYNS010000172         4294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_662224, whole genome shotgun sequence.
ACCESSION   DASYNS010000172 DASYNS010000000
VERSION     DASYNS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662224"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /gene="argJ"
                     /locus_tag="VGP50_05440"
     CDS             complement(<1..1069)
                     /gene="argJ"
                     /locus_tag="VGP50_05440"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEV8026849.1"
                     /translation="MSQQATIPQRAEESAGAKPATSSKASPLAPARLPELPPIAGVTL
                     ATAHCGIRYPGRRDLMVALLEAGTSVAGVLTRSLTPGAPVDWCRGALPRGKARAAVVN
                     SGNANTFTGRAGARIVADTASAMARLVDCHPREVYVASTGVIGELPPADKIIGALPAT
                     ARSASPTAWLEAAQAIMTTDTFPKLATRRAEIDGAPVTINGFAKGSGMIAPDMATMLA
                     FLFTDAALPAKLLQPLLAAANERSFNSITVDGDTSTSDTALLFATGKAQHKRVTRLAD
                     HRLKSFRRALDEVMIDLAQQIVRDGEGAEKFLTIEVKGAASAKAARRIGLAIGNSPLV
                     KTAIAAGDANWGRIVMAVGKSG"
     gene            complement(1066..2049)
                     /locus_tag="VGP50_05445"
     CDS             complement(1066..2049)
                     /locus_tag="VGP50_05445"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV8026850.1"
                     /translation="MTLRAILALCFALLAAPAWAQAAKPAKPAAAANDPVVARVNGTE
                     LHRSDAVELMRSLPPQLQKQPPDKLYPMVLDQMVGTLLVSQAGRKSKLQDDPIVKKRL
                     ALAQDQIIADAYVQRLLAKSVTDDKLHARYDKIVKETPEREEVKARHILVPTEEEAKA
                     IIVQLKGGADFAKLAGEKTTDPAGKASGGDLGYFTKDEMVPEFADAAFKLKNGEYTET
                     PVKSQFGWHVIKVEDRRTAKPPSFDQMKPRLTNDVSREIVSEKIKELKVAAKIDVYNP
                     DGSKVGAAPPPAAAPAPDAPAAGAPTLAPETAPGAAPEGTPTLAPETKPPG"
     gene            2282..>4294
                     /gene="secA"
                     /locus_tag="VGP50_05450"
     CDS             2282..>4294
                     /gene="secA"
                     /locus_tag="VGP50_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV8026851.1"
                     /translation="MIGAIVRRAFGSANDRYVRKIQPIVDEINAAEPGVVALTDEDLR
                     ARTAALKQRLADGETLDDVLPEAFATVREAAKRTLGQRHFDVQLMGGVVLHRGMISEM
                     KTGEGKTLVATLPVYLNALTGKGVHVVTVNDYLARRDSEWMGQIYRFLGLSVGCIVHG
                     LDDEQRRAAYAADVTYGTNNEFGFDYLRDNMKFQLEHMVQRPFNFAIVDEVDSILIDE
                     ARTPLIISGPAEDSSVLYRTVDKLIPKLAAEDYEKDEKQRQVVLSESGVEKIEAMLRE
                     ADLLQSGTLYDIHNVALVHHANQALRAHKLFARDTEYIVKDGKIVIIDEFTGRMMEGR
                     RYSEGLHQALEAKEGVEIQNENQTLASITFQNYFRLYPKLAGMTGTAMTEAAEFGDIY
                     KLDVIEIPTNRPMIRVDNDDEVYRTAREKYEAIIEQIQDCLSRQQPVLVGTVSIEKSE
                     ALAAHLKKRKVAHQVLNARYHEQEAFIIAQAGRPGAVTIATNMAGRGTDIQLGGNLEM
                     RLKQELSGIEDEGEREKRKAEIRAEIEAAREEVRRSGGLFVIGSERHESRRIDNQLRG
                     RSGRQGDPGASKFFVSLEDDLMRIFGSERMDGMLQRLGLKEGEAIIHRWINKALEKAQ
                     QKVEARNYESRKYVLKFDDVMNDQRKVIYEQRKEIMSAKDVAQAIAD"
BASE COUNT          746 a         1417 c         1416 g          715 t
ORIGIN      
        1 cgccggactt gccgacggcc atgacgatgc ggccccaatt ggcgtcgccg gcggcgatcg
       61 ccgtctttac caggggcgag ttgccgatgg cgaggccgat gcgccgggcc gccttcgccg
      121 aggccgcgcc cttcacctcg atggtgagga atttctcggc gccttcgccg tcgcgcacga
      181 tctgctgggc aaggtcgatc atgacctcgt cgagcgcgcg gcgaaaggat ttgaggcggt
      241 gatcggcaag ccgcgtgacg cgcttgtgct gggctttgcc ggttgcgaaa agaagggccg
      301 tgtcgctggt ggaggtgtcg ccatcgaccg tgatcgaatt gaaggagcgc tcattggctg
      361 cggccagcag cggctgaagc agcttcgccg ggagcgccgc gtcggtgaag aggaaagcga
      421 gcatcgtcgc catgtccggc gcgatcatgc cggagccctt ggcgaagccg ttgatcgtca
      481 caggcgcgcc atcgatctcc gcccgccgcg tcgccagctt cggaaaggtg tccgtggtca
      541 tgatcgcctg cgccgcttcg agccaggcgg tcggtgaggc gctgcgcgcc gtcgccggca
      601 gcgcgccgat gatcttatcc gccggcggca gctcgccgat cacgccggtc gacgcgacat
      661 agacctcgcg gggatggcaa tcgacgagcc ttgccatggc cgatgccgtg tccgccacga
      721 tccgcgcacc cgcgcgaccc gtgaacgtgt tggcattgcc cgaattgacg accgcagcgc
      781 gggccttgcc gcgcggcaac gcgccgcggc accaatcgac cggcgcgccc ggcgtgagcg
      841 agcgggtgag cacgccggcg acgctcgtcc ctgcctcgag cagcgccacc atcagatcgc
      901 ggcggcccgg atagcgaatc ccgcaatgcg ccgtcgccag ggtgacgccg gcaatcggcg
      961 gcagctccgg caaacgcgcc ggcgccaggg gcgaagcttt ggacgacgtc gccggtttcg
     1021 cgccggcgct ctcttccgcg cgctgcggga tcgtcgcctg ttggctcatc ccggcggctt
     1081 ggtttcgggt gccagcgtcg gcgtgccttc cggcgccgca cccggagcgg tctcgggcgc
     1141 gagcgtgggc gcgcccgcag ccggggcgtc aggcgccggg gccgcggccg gcggcggtgc
     1201 cgcgccaact ttgctgccgt cgggattgta aacgtcgatc ttcgccgcga ccttcagctc
     1261 cttgatcttc tcgctgacga tctcgcgcga cacgtcgttg gtgagacgcg gcttcatctg
     1321 atcgaagctc ggcggcttcg ccgtgcggcg gtcctccacc ttgatcacgt gccagccgaa
     1381 ctggctcttc accggcgttt ccgtgtattc gccgtttttg agcttgaagg ccgcatcggc
     1441 gaattccggc accatctcgt ccttggtgaa gtaaccgaga tcgccgcccg aggccttgcc
     1501 ggctggatcg gtggtcttct cgccggcaag ctttgcaaaa tcggccccgc ccttgagctg
     1561 gacgatgatc gctttggcct cttcctccgt gggaaccagg atgtgccgcg ccttgacctc
     1621 ctcgcgctcc ggcgtctcct taacaatttt gtcataacgc gcatggagct tgtcgtcggt
     1681 gacgctcttc gcgaggaggc gctgcacata ggcgtcggcg atgatctggt cctgcgccag
     1741 cgccaggcgc ttcttcacga tcggatcgtc ctggagcttg ctcttgcgcc cggcttggct
     1801 caccagcagc gtccccacca tctggtcgag aaccatcggg tagagcttgt ccggcggctg
     1861 cttctgcagc tgaggcggca gcgaacgcat gagctcgacc gcgtccgagc ggtgcaattc
     1921 cgtgccgttg acgcgcgcca cgaccgggtc gttcgcggcg gcggccggct tggccggctt
     1981 ggccgcctgc gcccaggcgg gagcggcgag gagcgcaaaa cagagggcga ggatcgctcg
     2041 aagcgtcatg gagggttcct tattgatgca acaaccgcag ccaaaagccg cctgcgcccc
     2101 ctcgataccg gggcgctggg cgccataaag cacattgcct cgcggagaac aaggagaagc
     2161 ggcgcgcaag ctgacccatc ggcgggcccc gtcaattgac aggctcgccc tctacgccta
     2221 tctgtcagcc gggatcagcg cggcgactgg ctctcccgcc cgccacaagc ttgaggtctt
     2281 gatgatcggc gccatagtcc ggcgagcgtt cggctccgcg aacgatcggt atgtcaggaa
     2341 aatccagccc attgtcgatg agatcaatgc ggctgagccg ggcgtcgttg cgcttaccga
     2401 tgaggacctc cgggcgcgca cggccgcgct gaagcagcgc ctggccgatg gcgagaccct
     2461 cgacgacgtc ctccccgagg ccttcgccac ggtgcgcgag gcggccaagc gcacgctcgg
     2521 ccagcgccat ttcgacgtgc agctcatggg cggcgtcgtg ctgcatcgcg gcatgatctc
     2581 ggagatgaag accggcgagg gcaagaccct ggtcgcgacg ctgcccgtct atctgaacgc
     2641 gctgaccggc aaaggcgtgc atgtcgtcac ggtgaacgat tatctcgccc gccgcgacag
     2701 cgagtggatg gggcagatct accgtttcct cggtctttcc gtcggctgca tcgtgcacgg
     2761 cctcgatgac gagcagcgcc gcgccgccta tgccgccgac gtcacttacg gcaccaacaa
     2821 cgagttcggc ttcgattatc tgcgcgacaa catgaagttc cagctggaac acatggtcca
     2881 gaggcccttc aacttcgcga tcgtcgacga ggtggactcg atcctgatcg acgaggcgcg
     2941 cacgccgctg atcatctcgg gcccggccga ggactcctcc gtgctttatc gcacggtcga
     3001 caagctcatc ccgaagctcg ccgccgagga ttacgaaaag gacgagaagc agcgccaggt
     3061 ggtgctgtcg gaatcgggcg tcgagaagat cgaagccatg ctgcgcgagg ccgatctgct
     3121 ccagtcgggc acgctctacg acatccacaa cgtcgcgctc gtccatcacg ccaatcaggc
     3181 gctccgcgcg cacaagctgt ttgcccgcga caccgagtac atcgtcaagg acggcaagat
     3241 cgtcatcatc gacgagttca ccggccgcat gatggaaggg cggcgctact ccgaaggcct
     3301 gcaccaggcg ctcgaggcca aggaaggcgt cgagatccag aacgagaacc agacgctggc
     3361 gtcgatcact ttccagaatt atttccgcct ctatccgaag ctcgccggca tgaccggcac
     3421 ggcgatgacc gaggcggccg agttcggcga catctacaag ctcgacgtca tcgagattcc
     3481 gaccaacagg ccgatgatac gtgtcgacaa cgacgacgag gtctaccgta ccgcccgcga
     3541 gaaatacgag gccatcatcg agcagattca ggattgcctc tcgcgccagc agccggttct
     3601 cgtcggcacg gtcagcatcg agaagtcgga ggccctcgcg gcgcatttga agaagcgcaa
     3661 ggtcgcgcat caggtgctga acgcccgcta tcacgagcag gaggcgttta tcatcgcgca
     3721 ggcgggccgg cccggcgccg tcaccatcgc caccaacatg gcggggcgag gcaccgacat
     3781 ccagctcggc ggcaacctcg agatgcgctt gaagcaggag ctgagcggca tcgaggacga
     3841 aggggagcgc gagaagcgca aggccgagat tcgcgccgag atcgaggcgg cgcgcgaaga
     3901 ggtgcggcgc tccggcgggc ttttcgtcat cggctccgag cgccatgaga gccggcgcat
     3961 cgacaatcag ctgcgcggcc gctccggccg ccagggcgat cccggcgcgt ccaagttctt
     4021 cgtctcgctc gaggatgacc tcatgcgcat cttcggctcg gagcgcatgg acgggatgct
     4081 gcagcgcctc ggcctcaagg aaggcgaggc gatcatccat cgctggatca acaaggcgct
     4141 cgagaaggcg caacagaagg tcgaggcgcg caactacgaa tcacgcaaat acgtcctcaa
     4201 attcgacgac gtgatgaacg accagcgcaa ggtcatctac gagcagcgca aggagatcat
     4261 gtccgccaag gacgtggctc aggccatcgc cgac
//
LOCUS       DASYNS010000173         3784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5342002, whole genome shotgun sequence.
ACCESSION   DASYNS010000173 DASYNS010000000
VERSION     DASYNS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5342002"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /gene="aroC"
                     /locus_tag="VGP50_05455"
     CDS             complement(<1..564)
                     /gene="aroC"
                     /locus_tag="VGP50_05455"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV8026852.1"
                     /translation="MAGSSYGTLFRFTTWGESHGPAIGCVVDGAPPLLPLAESDIQHW
                     LDRRRPGQSRFTTQRHESDRVEILSGVFEGQTTGTPIMLLIRNEDARSKDYAAIKDSF
                     RPGHADYSYWKKYGIRDYRGGGRASARETASRVAAGAIARKILGPAVTIRGAVIQIGP
                     HKIARDRWDWAAVEDNPFWSPDRQMAQT"
     gene            complement(573..1388)
                     /gene="fabI"
                     /locus_tag="VGP50_05460"
     CDS             complement(573..1388)
                     /gene="fabI"
                     /locus_tag="VGP50_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEV8026853.1"
                     /translation="MVHVSPLMEGKRGLIMGVANERSIAWGIAKALHAQGAELAFTFQ
                     GEALGKRVLPLAQSLGAQLVLDCDVTDDSSLETVFRTLAKEWRRLDFVVHAIAFADSS
                     TLKGRYIDAPRAHFLRALEISCYSFTDVCRRAAPLMTEGGSLLTLTYYGAERVVPHYN
                     LMGVAKAALEASVRYLAVDLGGQNIRVNAISAGPIKTLSAKGGIGDFTYILKWNQYNS
                     PLKHNTTIEEVGGAGVYLLSGLSAGVTGEIHHVDSGYHIVGMKAIDAPDISLV"
     gene            1520..2851
                     /locus_tag="VGP50_05465"
     CDS             1520..2851
                     /locus_tag="VGP50_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEV8026854.1"
                     /translation="MMPAAGELRRLAAWLREAPRALWAFTGYAILRFGRDGCLAGAGA
                     LSYTTLVALVPTMAIALTVLSAVPVFAETRDDLLAKLFQSVVPQVGAEVEWWFRYFAG
                     TSVRTTTIGVLALAVTVVLLLATIEDQLHAIWHVTSPRPWVQRILAYWAILTLGPLLL
                     GVSFSLPGYLDVLARHAGLAAYEPSDLMGPTGLHRLLRFVPFLLETAAFTLLYALIPN
                     CSVRWREALLGAVVAAALLEALKTGFAVYIGYFSSYRAVYGALAAIPIFLVWMYVAWS
                     AVLFGAIVAAALPRWRVDEQAKNVRPAAHRLGLGLALLAELWAQTRHGGMLGTATLAQ
                     RLGIATSVVDDDLTRLRRSGFVAQTAEGGWVLARALDGAALVDLYRALDLPLAASLSE
                     EAGAPWQARIAPAIERIAAVERDALALPLDALVGSTAQIAPLPQPTRQRRP"
     gene            complement(2890..>3784)
                     /locus_tag="VGP50_05470"
     CDS             complement(2890..>3784)
                     /locus_tag="VGP50_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEV8026855.1"
                     /translation="SGDVSDSTVMTKDPYAILGVARNASAADIKAAYRRLAKRLHPDI
                     SPGNDAHEQRFKEVASAYDLLSDPQKRARFDRGEIDAVGAERGWGSRPGGTGRRGPDS
                     NGGVSLDEILAEVLGRRRRDSHAAGERAGNATETLRLGFIEAALGGKRVIALGDGRKV
                     EITVPSGVESGQRLRLRHGPLQETYLEIEVEAHPLFRRDGRDVHVDLPVTLPEAVLGA
                     VVTVPTIHGDVALKVPPGSNSGATLRLRAKGIPAPQGHGDQYVKLRVVLPDPPDPELV
                     AFLEHWGARHPYQVRGKRRNG"
BASE COUNT          538 a         1310 c         1299 g          637 t
ORIGIN      
        1 ggtctgcgcc atctgccggt cgggactcca gaagggattg tcctcgaccg cggcccagtc
       61 ccagcggtcg cgcgcgatct tgtgcggccc gatctggatg acggcgccgc ggatcgtgac
      121 ggcgggcccg aggatcttgc gcgcgatggc gccagcggcg acgcgggatg ccgtctcgcg
      181 cgccgaggcg cgcccgccgc cgcggtagtc gcggatgccg tatttcttcc agtagctata
      241 gtccgcgtga cccggccgga agctgtcctt gatcgcggcg tagtccttcg accgtgcatc
      301 ctcgttcctg atcaagagca tgatcggcgt tcccgtcgtc tggccttcga agacgccgga
      361 aaggatctcg acgcggtccg actcgtggcg ctgtgtggtg aagcgcgact gaccgggccg
      421 gcgccgatcg agccaatgct gaatgtccga ttcggcaagc ggcagcaaag gcggcgcgcc
      481 gtcgacgacg caaccgatgg ccggcccgtg gctttcgccc caggtggtga agcgaaacaa
      541 ggtcccgtag ctgctgcccg ccacggcgac tcctacacga gtgaaatgtc gggcgcgtcg
      601 atcgccttca tgccgacgat gtggtagccg ctgtcgacat ggtggatctc gcctgtgacg
      661 ccggcggaaa ggccgctcag caggtaaacc ccggcgccac ccacctcctc gatggtagta
      721 ttgtgcttca acggcgaatt atactggttc cacttgagga tataagtgaa gtcgccgatt
      781 ccgcccttgg cggagagggt cttgatcggg ccggcggaga tggcgttgac gcgaatgttc
      841 tggccaccca gatcgacggc aaggtagcgg acgctcgcct cgagcgccgc cttggccacg
      901 cccatgaggt tgtaatgggg caccacgcgt tcggcgccgt aataggtcag cgtcaaaagg
      961 ctgccgcctt ccgtcatcaa cggcgccgcg cggcggcaga catcggtgaa ggagtagcag
     1021 gatatctcga gcgcgcgcag gaagtgggca cgaggtgcgt cgatgtagcg gcccttgagc
     1081 gtgctcgaat cggcgaaagc gatggcatgg acgacgaaat cgaggcgccg ccattccttg
     1141 gcgagcgtgc ggaagaccgt ctcgaggctc gaatcgtcgg tgacgtcgca atcgagcacc
     1201 agctgcgcgc cgaggctctg ggcgagcggc agcacgcgct tgccgagagc ttcgccctgg
     1261 aaggtgaaag cgagctcggc gccttgcgca tgaagcgcct tggcgatgcc ccaggcgatc
     1321 gagcgctcat tggcgacacc catgatgagc ccgcgtttgc cttccatcag cggcgacaca
     1381 tgcaccatac ctcgatgact cctcggccaa tcgccttgaa cccgacaaag ggcgtgcatc
     1441 ctacacgaag gagaaggggc gcggaaagcg ccccttcggc cgggtggcgt gtgcgcgatg
     1501 gcgccgggcg ggcggcgcca tgatgcccgc cgccggggag cttcgccggc tggctgcgtg
     1561 gctgcgcgaa gctccgcgcg cgctctgggc tttcaccggc tacgccatcc tgcgcttcgg
     1621 ccgcgacggc tgcctcgccg gcgccggcgc gctcagctac acgacgctcg tggcgctggt
     1681 gccgacgatg gcgatcgcgc tcaccgtcct ctcggccgtg cctgtcttcg ccgagacgcg
     1741 cgacgatctc ctcgcgaagc tcttccagag cgtggtgccg caggtcggcg ccgaggtcga
     1801 gtggtggttc cgctatttcg ccggtacctc ggtgcgcacc acgacgatcg gcgtcctcgc
     1861 tctcgccgtc accgtcgtgc tgctcctcgc caccatcgag gaccagctcc acgcgatctg
     1921 gcacgtcacc tcgccgcggc cctgggtgca gcgcattctc gcgtattggg cgatcttgac
     1981 gctgggtccc cttctgctcg gcgtcagctt ctcgctgccc ggctatctcg acgtgctcgc
     2041 gcgccacgcc ggtctcgcgg cctatgagcc gagcgatctc atggggccga ccggactcca
     2101 tcgcctgctc cgcttcgtgc cgttcctgct cgaaacggcc gcgttcacgc tgctctacgc
     2161 gctcattccc aactgctcgg tgcgctggcg cgaggcactg ctcggtgccg tcgtcgccgc
     2221 cgctctgctc gaggcgctga agaccggctt cgccgtctat atcggctact tctcgagcta
     2281 ccgcgccgtc tacggcgcgc tcgccgccat cccgatcttc ctcgtctgga tgtatgtcgc
     2341 ctggagcgcg gtgttgttcg gcgcgatcgt ggcggcggcg ctgccgcgat ggcgcgtcga
     2401 cgagcaggcg aagaatgtgc gtccggcggc gcatcgtctc ggtctcggcc tcgcgctgct
     2461 cgcggagctg tgggcacaga cgcgccacgg cggcatgctc ggcaccgcca cgctggccca
     2521 gcgtctcggc atcgcgacca gcgtcgtcga tgacgatctg acgcggttgc gccgcagcgg
     2581 cttcgtcgca cagaccgccg aaggcggctg ggtgctggcg cgcgctctcg acggcgccgc
     2641 cctggtcgat ctctatcgcg cgctcgacct gccgctggcg gcgagcctca gcgaagaggc
     2701 gggcgcgccc tggcaggcgc gcatcgctcc ggcgatcgag cgcattgccg cggtcgagcg
     2761 cgacgcgctc gcgctgccgc tcgacgcgct cgtcggcagc accgcgcaga tcgcgccgct
     2821 gccccagccg acgcgccagc gacgcccctg aggcggtgcg ctgcgccgga ccggcgattg
     2881 ccgccggcct cagccgttgc ggcgcttgcc gcgcacctga tagggatgcc gcgctcccca
     2941 atgctcgagg aacgcgacaa gctcgggatc gggcgggtcg gggagaacga cgcgcagctt
     3001 cacatactgg tcgccatggc cctgcggcgc cggaatgccc ttggcgcgca ggcgcagcgt
     3061 cgcaccgctg ttcgagccgg gcggcacctt gagcgcgacg tcgccgtgaa tggtcggcac
     3121 cgtgacgacg gcgccgagca ccgcttcggg aagcgtcacg ggcaggtcga catgcacgtc
     3181 gcgtccgtcg cggcggaaga gcgggtgagc ctcgacctcg atctcgagat aggtctcctg
     3241 caaggggccg tggcgcaatc tcagccgctg gccgctttcg acgccggacg gcacggtgat
     3301 ctcgaccttc cgcccgtcgc cgagcgcgat cacccgcttg ccgccgaggg ccgcctcgat
     3361 gaagcccagg cgcagcgtct cggtggcgtt gcccgcgcgc tcgccggccg catggctgtc
     3421 gcgacgacgg cggcccagca cttcggcaag gatctcatcg agcgagacgc cgccattgct
     3481 gtccgggccg cgccgccccg tgccgcccgg gcgcgagccc cagccgcgct cggcgccgac
     3541 cgcgtcgatc tcgccgcggt cgaagcgggc gcgcttctgc gggtccgaga gcagatcgta
     3601 agccgacgcg acctctttga agcgctgctc atgggcgtcg ttgcccgggc ttatgtcggg
     3661 atgaaggcgt ttcgcgagcc ggcgataagc cgccttgatg tcggccgcgg aagcatttcg
     3721 cgcgacaccg agaatggcgt aagggtcctt cgtcattacc gtactgtccg atacgtcgcc
     3781 tgac
//
LOCUS       DASYNS010000174         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3003446, whole genome shotgun sequence.
ACCESSION   DASYNS010000174 DASYNS010000000
VERSION     DASYNS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3003446"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VGP50_05475"
     CDS             complement(<1..331)
                     /locus_tag="VGP50_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV8026856.1"
                     /translation="MMLQGMKPQGLLSIGEAAAHSGVPAKTIRFYEEAGIIKPAQRGE
                     NGYRVYSEADVQTLHFIHRSRALGFRLKDVAELLELYRNRKRASREVKKLALEHVAEL
                     DRKIAEMT"
     gene            complement(285..2777)
                     /locus_tag="VGP50_05480"
     CDS             complement(285..2777)
                     /locus_tag="VGP50_05480"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV8026857.1"
                     /translation="MNANVKLSELADGRSRHVVLSIEGMTCATCVARVEKALDALPGV
                     TALVNLACERADVDFDPARVGPGMLVEAVERAGYEVSHETRELAISGMSCASCAGRVE
                     RALAAVPGVVNASVNLASDKATVEAIAGVLRPADLIDATRRAGYDAELLTGEAERERL
                     MAVAEETRLQSEGRRVIAACALGAPLLLPMLGVPLPPWLQLALATPVQFVLGWRFYVS
                     AFKALRARAGNMDLLVSLGTSAAYFYSLYLFFAGTSAEVGEHFYFEAAAVVIALVMLG
                     KWLEARAKRSTTAALRALMSLRPERARVEREGGEVEVPIADLSVTDIAVVRPGERLPA
                     DGIVLSGESEVDESLLTGESLPVAKVAGDEVTGGSINGSGLLRVETRAVGEQSMLARI
                     IKLVEAAQVKKAPVQRLVDRVAAIFVPIVLALAFASFLAWWLVVGDFASGIIAAVSVM
                     VIACPCALGLATPTALMVGTGVAAKAGILIRDAEALEQARAIDTVVLDKTGTLTEGKP
                     EVAEVVPHGIDERELLALVAAAQGGSEHPLARAVLAKVDGIESPPIERLPLEEFRARA
                     GLGVLARVGGREIAVGNRKLMQEQGVAIGPLAEAAETLETRGRTVIWAAALGAEPRLV
                     GIMALADRVKPSAVAAVRRLHESGIATILMTGDNERTANAVAATLGIERVMAGMLPAD
                     KAGEVERLKAQGKHVAMVGDGVNDAPALAAADVGIAMGSGADVAIETAGIALMRGDPM
                     LIADAIAVARATYAKIRQGLFWAFIYNLIGLPLAASGLMSPMLAGAAMALSSVSVVSN
                     ALLLRQWAPASEKRSRRHDVARDEAARVAEHR"
     gene            complement(2793..3020)
                     /locus_tag="VGP50_05485"
     CDS             complement(2793..3020)
                     /locus_tag="VGP50_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEV8026858.1"
                     /translation="MDELARRNGVGSGVALTITGMSCGGCVGAVKRLLSKVPGVSDVD
                     LGAGRALVAGTARAEDLVAALQGTGYAARPG"
BASE COUNT          493 a         1086 c         1007 g          464 t
ORIGIN      
        1 tcgtcatctc ggcgatcttg cggtcgagct cggcgacgtg ctcgagcgcg agcttcttca
       61 cttcccggct cgcgcgcttg cggttgcggt agagctcgag aagctcggcc acatccttca
      121 gccggaagcc gagcgcgcgc gagcgatgga tgaagtgcaa ggtctgcaca tcggcctcgc
      181 tatagacccg atagccgttc tcgccgcgtt gcgccggctt gatgatgccg gcttcttcgt
      241 agaagcggat cgtcttcgcc ggcacgcccg aatgagcggc ggcttcaccg atgctcagca
      301 acccttgcgg cttcatccct tgcaacatca tgacggcggc tcctcttctc gcttgccggc
      361 gcccattggc gcaacagcag cgcattcgag acgacgctca ccgaggaaag cgccatggcg
      421 gcgccggcga gcatcgggct catgaggcct gaggcggcga gcggcaggcc gatgagattg
      481 tagatgaagg cccagaaaag tccctggcgg atcttcgcgt aagtggcacg ggcgacggcg
      541 atggcgtcgg cgatcagcat cgggtcgccg cgcatgagcg cgatgcccgc cgtctcgatc
      601 gccacatcgg cgcccgagcc catggcgatg ccgacatcgg ctgccgccaa ggcgggcgcg
      661 tcgttcacgc cgtctcccac catcgcgacg tgcttgcctt gcgccttcag ccgctcgacc
      721 tcgcccgcct tgtcggcggg cagcatgccg gccatgacgc gctcgatgcc gagcgttgcc
      781 gccacggcat tcgcggtccg ctcgttgtcg ccggtcatca agatcgtcgc gatcccgctt
      841 tcgtgcaggc ggcggaccgc cgctaccgcc gatggcttca cgcggtcggc gagcgccatg
      901 atgccgacga ggcgcggctc ggcgccaagc gccgcggccc agatgacggt gcgacccctg
      961 gtttcgagcg tctcggccgc ctccgccaaa gggccgatcg ccaccccttg ctcttgcatg
     1021 agcttgcggt tgcccaccgc gatctcccgg ccgccgacgc gcgcgagcac gccgaggccc
     1081 gcgcgggcgc ggaattcctc caagggcaaa cgctcgatgg gagggctctc gatgccgtca
     1141 accttcgcca gcacggcacg tgccagagga tgctcgctgc cgccttgcgc ggcggcgaca
     1201 agcgccagca attcgcgttc gtcgatgccg tgcggcacca cctccgccac ctccggcttt
     1261 ccttcggtga gcgttcccgt cttgtcgagc acgaccgtgt cgatggcacg cgcctgctcc
     1321 agcgcctcgg cgtcacgaat caagatgccc gccttcgcgg cgacgcccgt gccgaccatc
     1381 aaggcggtcg gcgtcgcgag tccgagcgcg caggggcagg cgatcaccat caccgaaacc
     1441 gcggcgatga tgcctgaggc gaaatcgccg acgacgagcc accaggcgag aaagctcgcg
     1501 aaggcgaggg cgagcacgat cggcacgaag atggcggcga cgcggtcgac gaggcgctgc
     1561 accggcgcct tcttcacctg cgcggcctcg acgagcttga tgatacgcgc cagcatcgac
     1621 tgctcgccga ccgcgcgcgt ctcgacgcgc aagaggccgc tgccgttgat cgagccgccg
     1681 gtcacctcgt cgcccgcgac tttcgctacg ggcaggcttt cgccggtgag aaggctctcg
     1741 tcgacctcgc tttcgccgga gagcacgatg ccgtcggcgg gtagccgctc gcctggccgc
     1801 accacggcaa tgtcggtgac ggaaagatcg gctatcggca cctcgacctc accgccttcg
     1861 cgctcgaccc gcgcgcgttc gggcctgagc gacatcaggg cgcggagcgc cgcggtcgtc
     1921 gaacgcttcg cgcgcgcctc gagccatttg ccgagcatga cgagcgcgat caccaccgcg
     1981 gccgcctcga aataaaaatg ctcgcccacc tcggccgagg tgccggcgaa gaagagatag
     2041 aggctgtaga aataggcagc cgaggttccg agcgacacca ggaggtccat gttgccggcg
     2101 cgcgcgcgca gcgccttgaa ggccgagacg tagaagcgcc agccgagcac gaattgcacc
     2161 ggggtcgcga gcgcgagctg aagccacggg gggagcggca cgccgagcat cggcagcaag
     2221 agcggcgcgc cgagcgcgca agccgcgatg acccgccgtc cctcgctctg caaacgcgtt
     2281 tcctcggcga cggccatcag acgctcgcgc tcggcctcgc ccgtcagcag ctcggcgtca
     2341 tagccggcgc ggcgcgtggc atcgatgaga tctgccgggc gcagcacgcc cgcaatcgcc
     2401 tcgaccgtgg ccttgtcgct tgcgagattg accgaggcat tgacgacgcc aggaaccgcc
     2461 gcgagcgcgc gttcgacgcg gccggcgcag gaggcgcagc tcattccgga gatggcgagc
     2521 tcgcgcgtct cgtgcgacac ctcgtagccg gcgcgctcga ccgcttcgac gagcatgccc
     2581 ggcccgacgc gcgccgggtc gaaatcgaca tccgcgcgtt cacacgcgag gttcacgagg
     2641 gcggtgacgc cgggcaaggc atcgagcgcc ttctcgacgc gcgccacgca agtggcgcaa
     2701 gtcatgccct cgatcgaaag gacgacgtgc cgcgagcggc catcggccaa ttcgctcagc
     2761 ttcacattcg cgttcatggc gttgatgccg cttcagccgg gccgcgcggc atagccggtg
     2821 ccttgcagcg ccgcgacaag atcctcggca cgtgccgtgc ctgcgacaag cgcgcgtccg
     2881 gcgccgagat cgacgtcgga gacgcccggc actttcgaca gcagccgctt tacggcaccg
     2941 acacagccgc cgcagctcat gccggtgatc gtaagtgcta cgcccgagcc gacgccgttc
     3001 cttctagcca attcgtccaa gacggacctc cctgccttcc atttgcaggg
//
LOCUS       DASYNS010000175         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3627747, whole genome shotgun sequence.
ACCESSION   DASYNS010000175 DASYNS010000000
VERSION     DASYNS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3627747"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1601)
                     /locus_tag="VGP50_05490"
     CDS             complement(93..1601)
                     /locus_tag="VGP50_05490"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV8026859.1"
                     /translation="MTMALESTARRQPADLTPAAPPYRTAPHNIELEQALLGAILVNN
                     EAFYRVSDFLEPRHFFEPIHQKLYEITASLVRIGKTATPITLKTFLPADLDVAGLTAS
                     QYLARLAAEATTVINASDYGRTIYDLAIRRSLIGIGEEMVNAAYDAPVDERPQDQIEE
                     AERSLYELAETGRYDGGFQRFAQALTTAVEMAAHAYQRDGKLSGLATGLKDLDRMMGG
                     LQKSDLLILAGRPGMGKTALATNLAYNVAKAWEGAVRADGHIESTSGGIVGFFSLEMS
                     AEQLATRILSEQTEIASYRIRRGEIEPSDFDRIALTARDMEQMPLYIDETGGLSIAQL
                     AARARRLKRQRGLDLLVIDYIQLLSGSSRRAQEGRVQEVTEITTALKALAKELNIPIL
                     ALSQLSRAVESRDDKRPQLSDLRESGSIEQDADVVMFVFREEYYLKNKEPRPGSEEHF
                     KWQTEMDAVHGRAEVIIGKQRHGPTGTVGMQFKADVTRFSDLAEGDLLPERM"
BASE COUNT          233 a          529 c          623 g          293 t
ORIGIN      
        1 taccggctgg gcgcgttccc gccgtgaacg ccgtccccgc attgcgccgc aagggcggct
       61 ccatgcgggc tacgggcgta acgcgcggcg cctcacatcc gttccggcag caaatccccc
      121 tccgccaagt cggagaaccg cgtcacgtcg gccttgaact gcatgcccac ggtgccggtc
      181 gggccgtggc gttgcttgcc gatgatgacc tcggcgcggc cgtggacggc gtccatctcg
      241 gtctgccact tgaagtgctc ttcgctgccc ggccgcggct ccttgttctt gaggtagtat
      301 tcctcgcgga acacgaacat caccacgtcg gcgtcctgct cgatcgagcc ggattcgcgc
      361 aggtccgaga gctgcgggcg cttgtcgtcg cggctttcga ccgcgcgcga gagctgcgac
      421 agcgccagga tcgggatgtt gagctccttg gccagcgcct tgagggcggt ggtgatctcg
      481 gtcacctcct gcacgcggcc ctcctgggcg cgacgcgagg agcccgacaa gagctggatg
      541 tagtcgatca ccaggaggtc gaggccgcgc tggcgcttga ggcggcgcgc gcgcgcggcg
      601 agttgggcga tcgagaggcc cccggtctcg tcgatgtaga gcggcatctg ctccatgtcg
      661 cgcgccgtca aggcgatgcg gtcgaagtcg gaaggctcga tctcgccgcg ccggatgcgg
      721 taggaggcga tctcggtctg ctccgagagg atgcgggtgg cgagctgctc ggccgacatt
      781 tcgagcgaga agaagccgac gatgccgcca ctggtggatt cgatgtggcc gtcggcgcgc
      841 accgcgccct cccacgcctt cgccacgttg taggcgaggt tggtggcgag cgcggtcttg
      901 cccatgccgg ggcggccggc gaggatgagg aggtcggact tctgcaagcc ccccatcatg
      961 cggtcgagat ccttgagccc ggtggcgagc cccgacagct tgccgtcgcg ctggtaggcg
     1021 tgcgccgcca tctcgaccgc ggtggtgagg gcctgggcga agcgctggaa gccgccgtcg
     1081 tagcggccgg tctcggcgag ctcgtagagg ctgcgctcgg cctcttcgat ctggtcctgc
     1141 ggacgctcgt cgacgggggc gtcataggcg gcgttgacca tctcctcgcc gatgccgatc
     1201 agcgagcggc ggatggcgag gtcgtagatg gtgcggccgt agtcggacgc gttgatgacg
     1261 gtcgtcgcct cggcggcgag gcgggcgagg tattggctcg ccgtgaggcc ggcgacgtcg
     1321 aggtcggcgg gcaggaaggt cttgagcgtg atcggggtcg cggtcttgcc gatgcgcacc
     1381 aggctcgcgg tgatctcgta gagcttctgg tggatcggct cgaagaaatg ccgcggctcg
     1441 aggaagtccg acacccggta gaacgcctcg ttgttcacca ggatggcgcc gagcagcgcc
     1501 tgctccagct cgatgttgtg cggagcggta cgatagggcg gtgcagcggg cgtgagatca
     1561 gccggctgcc tgcgggcagt cgattcgagc gccatggtca tgctgcctcc cgaatcgctt
     1621 cgtccacgac gcagatggct tggcgagcca tcgcggcctt gggaagggcc gcgccacg
//
LOCUS       DASYNS010000176         5773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5499371, whole genome shotgun sequence.
ACCESSION   DASYNS010000176 DASYNS010000000
VERSION     DASYNS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5499371"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1494)
                     /locus_tag="VGP50_05495"
     CDS             complement(229..1494)
                     /locus_tag="VGP50_05495"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV8026860.1"
                     /translation="MKLIKSGIVLPPRVAALALGRRVAALGDVGRRGISTARFAHIAT
                     WYQARVDGGDLPGAVIAIAHDGKLALLHAAGFKDHAKHRPMQPDAIFWIASMTKPITS
                     VGAMILAQEGTLDLDEPVDRYLPELKHKLVATEDGDPAKGRARLELVPQERAMTVRDL
                     LRHTSGLVYPPQYSDSSLHQLYGEKAVFARNRTLAEFVASLSALPLAHQPGTVWEYSW
                     GVDVLARVVEVVSGRRFDKFLEDRIFAPLQMVDTSFHVSQAKLARLVDPPAGGRPALW
                     DVTKSARLFSGGGGLVSTAMDYLRFCQMLLNRGELDGARILAPETVDAMTTNSLSPTI
                     RFAQDMVGPEAGCSWGLGVAIRTVAERSHVPGRVGSYSWGGVWGTLFWIDPAEKLAAV
                     LMIQVPPGESAPYRDALATLIYGALHPPL"
     gene            complement(1760..2368)
                     /locus_tag="VGP50_05500"
     CDS             complement(1760..2368)
                     /locus_tag="VGP50_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEV8026861.1"
                     /translation="MIAERQRFTLVQRLLHWLMALFILAMLFIGVGMVSTVMPKYLTL
                     VSIHKPLGIAILVLAVIRLAVRLRSGAPPLPADLPAPMKRAAELSHYALYALMIAMPL
                     IGWAMLSAAAYPVTLFPGLILPAILPPSDRLHALLWSAHFYLAFAFFALILLHLAAAL
                     FHALVRRDGVFQSMAPVELGGTASVKTTTPTRVPGSKPMRVP"
     gene            complement(2365..3426)
                     /locus_tag="VGP50_05505"
     CDS             complement(2365..3426)
                     /locus_tag="VGP50_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HEV8026862.1"
                     /translation="MTRPDPSPARSVFGSLLVIALVVGGAAAAFAYTAGWLSPERITP
                     DKLVDAFTPPGSAPPLGHRRNHAKGICFTGTFDANGAGAALSEAQVFARGRYPALGRF
                     NLATVEPDARDGTVRVRGMGLRIATPDGQEWRTAMIDAPVFTVSTPQAFYQLLLASGS
                     KDPEAMPTFVAAHPEFAAFIAWAKSAPWTGSYAEERYNSLNSFVFVDRAGGERTVRWS
                     LLPAAHPVPLSPEDLAKQGPDILEQEIAERVRGAPQRWSMVVTVADPGDPTADPTKAW
                     PPDRRSVEVGTLVVEQIVAEPDGPCRDINFDPTVLPTGIHTSDDPFPAARSSAYAKSY
                     DRRTAEAADYPRAAAEAKP"
     gene            3696..4679
                     /gene="bioB"
                     /locus_tag="VGP50_05510"
     CDS             3696..4679
                     /gene="bioB"
                     /locus_tag="VGP50_05510"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEV8026863.1"
                     /translation="MTIADPRAEIPGTPRHDWTRAEIGALFDLPLAELMFRAQTAHRR
                     HFDPREVQISTLLSIKTGGCPEDCAYCPQSAHYETGVAASKLMSVDAVLAEASAAKAA
                     GATRFCIAAAWRSPRDHDLDAVCAMVEGIKELSLESCATLGMLTADQAGRLKQAGLDY
                     YNHNLDTSPEYYERIITTRTYEDRLDTLAHVRAAGIHVCCGGIIGMGETRDDRIGMIA
                     ALATLPKHPESVPINLLAQVEGTPVSRGQKLDPLEFVRTIAVARLTMPASVVRLAAGR
                     PEMSDETQALCFLAGANSIFYGRDMLLTTANPGERHDRSLLDRLGMVAMPG"
     gene            4839..5468
                     /locus_tag="VGP50_05515"
     CDS             4839..5468
                     /locus_tag="VGP50_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV8026864.1"
                     /translation="MLCALSAQQAAAQTASNFYVRVDAGGSFPIDTTLAGEQNFSPSA
                     MVGGGVGFKILPFLRTDVTASYRTDYNENATDPNTFLGQKSEIKSLAGFFNGYFDIPT
                     GPLPIKPYIGAGVGAARNQTGTTSQFDPFTGNTASVDGATKMSFAYQAMLGVGFPIFP
                     GITIDAAYHYVDLGRFTTAGGVSLNGTPGVPLTGRLKANEIQLGLRVGF"
     gene            5623..>5773
                     /locus_tag="VGP50_05520"
     CDS             5623..>5773
                     /locus_tag="VGP50_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV8026865.1"
                     /translation="MAQHDFDMFTIGAGSGGVASSRRAGSYGARVAICENSRVGGTCV
                     IRGCVP"
BASE COUNT          988 a         1938 c         1953 g          894 t
ORIGIN      
        1 cgaccatgag gagcgcgatc ccgtgcaaca aagccgcggc caagagggcc cgtggtccaa
       61 gccattcgtt tgtccggtgc tcgtggcgca aggcgctccc gtaaacctgc ggcacggcgc
      121 gtgcccaggg ggcctcgccc gcacctattc cgccccgacg acaaacgatc atcgaccgtg
      181 cccggttcct ccgatcgcgg tggcgcccac ggggctcgtt cgccgggttc acaggggcgg
      241 gtgcaacgcg ccatatatca gtgttgcaag ggcatcacga tagggcgcgg actcgccggg
      301 cggcacctgg atcatcagca ccgcggccag cttctcggca ggatcgatcc aaaacagggt
      361 gccccatacc ccgccccagc tgtagctgcc gacccttccc ggcacgtggc tgcgctcggc
      421 aacggtgcgt atcgcgactc ccaatcccca actgcagccg gcctccggac cgaccatatc
      481 ctgcgcaaag cggatcgtcg gcgacaagga attcgtggtc atggcatcca ccgtttccgg
      541 cgccaggatc cgcgctccat cgagctcgcc gcgattgagc agcatctggc agaagcgcag
      601 gtagtccatg gcggtcgaaa cgagcccgcc gccgcccgaa aacaggcggg cggatttggt
      661 gacgtcccat agcgccgggc gtccgccagc gggtggatcg acgaggcgtg ccagcttcgc
      721 ttgcgacaca tggaagctcg tatccaccat ctgcaatgga gcgaaaatgc ggtcctccag
      781 gaatttatcg aagcgacgtc ccgacacgac ttcgacgacc cgcgccagca cgtcgacacc
      841 ccaactgtat tcccataccg tcccgggctg gtgcgccagc ggcagcgcgc tgaggctcgc
      901 gacgaactcc gccagggtcc tgttgcgcgc gaagacggcc ttctcgccgt agagctggtg
      961 gagcgacgag tccgaatatt gtggcggata gacgaggccg gaggtgtgac ggaggaggtc
     1021 gcggacggtc atcgcgcgct cttgaggcac gagctcgagg cgcgctctgc ctttcgccgg
     1081 atcgccgtct tcggtggcga cgagcttgtg cttcagttcc ggcagatatc gatccaccgg
     1141 ctcgtcgagg tcgagcgtcc cctcctgagc cagaatcatc gccccgacgc tcgttatggg
     1201 cttcgtcatc gacgcaatcc aaaagattgc atccggctgc atcggtcggt gttttgcgtg
     1261 gtccttgaag cctgccgcat gcaataaggc aagtttgccg tcatgcgcga tcgcgataac
     1321 ggcgcccggg aggtcgccgc cgtcgacgcg cgcctgatac caggtcgcga tatgcgcaaa
     1381 ccgagcggtc gaaatgccgc ggcgacccac gtcgccgagc gccgcgacac gccggccaag
     1441 tgcgagcgcc gccacgcgcg gtggcagtac gattccactt ttgatcagct tcacggattg
     1501 ccctcgatct gcatctcttc gcgacgatcg gcgaccgtgc cgggcggggc gagaacaata
     1561 ccgtgcaacc ataacgtcgt cagtcgcatc ggccaagcca gccccggggc gaccacaccg
     1621 ggatgacgac aaaagcgtgt gccgtgagac gcggcgccgc gatccgtcgg agattcgctt
     1681 ttgcgagtcg gttcgcattc gaacgggaat gcccggtcgt ttttcggtag cccggctata
     1741 gcagccgaca tgacgggggc tacggtaccc tcatcggctt cgatccgggg acgcgcgtgg
     1801 gcgtcgtcgt tttgaccgac gccgtgcctc ccaactcgac gggcgccatg gactggaaca
     1861 cgccgtcgcg ccgcacgagc gcatgaaaga gcgccgccgc gagatgcagc aggatcaagg
     1921 cgaagaacgc gaaggcgaga tagaaatgcg cgctccagag cagcgcgtga aggcggtcgc
     1981 tcggcggcag gatcgccggc aggatcaggc ccgggaacag cgtcaccggg taggcggcgg
     2041 ccgacagcat ggcccagccg atcagcggca tggcgatcat gagcgcatag agcgcgtaat
     2101 gcgacagctc ggccgcgcgc ttcatcggcg ccggcaggtc ggcgggcaga ggcggcgcac
     2161 cggagcgcag ccgcaccgcg aggcggatca ccgcgagcac caggatggcg atgccgagcg
     2221 gcttgtgtat tgacacgagc gtcagatatt tcggcatcac cgtggacacc atgccgacgc
     2281 cgatgaacag catggccagg atgaagagcg ccatgagcca atgcagcagg cgctgcacga
     2341 gggtgaagcg ctggcgctcg gcgatcacgg cttcgcctcc gccgccgctc ggggatagtc
     2401 cgcggcctcg gccgtgcgac gatcatagga cttggcatag gcggaggagc gcgcggccgg
     2461 gaagggatcg tccgaggtgt ggatgcccgt cggcagcacg gtcggatcaa aattgatgtc
     2521 gcggcagggg ccgtccggct cggccacgat ctgctcgacg acgagcgtgc cgacctccac
     2581 gctgcgccgg tccggcggcc aagccttggt cggatccgcg gtgggatcgc cgggatcggc
     2641 gacggtgacc accatgctcc agcgctgcgg cgcgccgcgg actcgttcgg cgatctcctg
     2701 ctcgaggatg tcgggccctt gcttcgcaag atcctcgggc gagagcggaa ccggatgcgc
     2761 cgccggcaga agcgaccatc gcaccgtcct ctctccgccc gcgcggtcga cgaagacgaa
     2821 gctgttcaga ctgttgtagc gctcctcggc gtagctgccg gtccagggcg cgctcttggc
     2881 ccaggcgatg aaggccgcga actcgggatg cgcggcgacg aaggtcggca tcgcttcggg
     2941 atccttgctc cccgaggcca gcaggagctg gtaaaaggcc tgcggcgtcg acaccgtaaa
     3001 gaccggggcg tcgatcatgg cggtgcgcca ttcctggccg tcgggcgtcg cgatgcgcag
     3061 ccccatgcca cgaacgcgga ccgtgccgtc ccgcgcgtcc ggctcgaccg tggcgaggtt
     3121 gaagcgcccg agggcgggat agcggccacg ggcgaacacc tgcgcctcgg agagcgccgc
     3181 gccggcgcca ttcgcgtcga aggtgccggt gaagcagatg cccttggcat gattccggcg
     3241 atggccgagc ggcggcgcgc tgcccggcgg cgtgaacgca tccaccagct tgtccggggt
     3301 gatgcgctcg ggcgagagcc atcccgcggt ataggcgaag gcggcggcgg cgcccccgac
     3361 gacgagcgcg ataaccagca gcgagccaaa cacggaacgc gccggcgaag ggtccggccg
     3421 ggtcattcga acaatccctg gtgagcctcg gacggagagc agggatatca gctttcgccg
     3481 ccgcgcgcct atcggccgca gggataagag accccaacca tcggacaagg cggccagggc
     3541 cataggtgag acgcgatgcg ctcggcggca tgctcgccgg ctcgcgctcg ttttcgggag
     3601 ccgccgcctg cgcgaagggc ggcttgacgc gccgcgggga taacggtact ccggctcaac
     3661 cgcgccgccg tccctgtcaa gcaagaggtt tccccgtgac gatcgccgac ccccgcgccg
     3721 agatccccgg gacaccgcgc catgactgga cgcgcgcgga gatcggcgcg ctcttcgact
     3781 taccgctggc cgagctgatg ttccgcgcgc agaccgcgca tcgccggcat ttcgatccgc
     3841 gcgaggtgca gatctcgacg ctgctttcga tcaagaccgg cggctgcccc gaggactgcg
     3901 cctattgccc gcaaagcgcc cattatgaga cgggcgtcgc ggcatccaag ctgatgagcg
     3961 tcgatgccgt gctggccgag gcaagcgccg ccaaggcggc gggtgcgacg cgcttctgca
     4021 ttgccgcagc ttggcgctcg ccccgggatc acgacctcga cgccgtctgc gccatggtcg
     4081 aaggcatcaa ggagctgtcg ctcgaaagct gcgccacgct cggcatgctc acggccgatc
     4141 aagccggccg cctcaagcag gccgggctcg actattacaa tcataatctc gacacctcgc
     4201 cggaatacta cgagcgcatc atcacgacgc gcacctacga ggatcggctc gacacgctcg
     4261 cccatgtccg tgccgcgggc atccatgtct gttgcggcgg catcatcggg atgggcgaga
     4321 cgcgcgacga ccgtatcggc atgatcgcgg cgctcgccac tttgccgaag catcccgaaa
     4381 gcgtgccgat caacctcctg gcgcaagtcg aggggacgcc ggtgagccgc gggcagaagc
     4441 tcgaccccct cgaattcgtg cgcaccatcg ccgttgcgcg tctcaccatg ccggcgtcgg
     4501 tggtgaggct ggcggcgggg cgcccggaaa tgagcgacga gacgcaggcg ctgtgcttcc
     4561 tcgccggcgc caattcgatc ttctacgggc gcgacatgct gctgacgacg gccaatcccg
     4621 gcgagcgcca cgaccgctcg ctgctcgacc ggctcggcat ggtggcgatg ccgggctgag
     4681 cgcgggccga tgttgcgacg ctgcaacagg gcgcgatatt cgcggggcgc gtcaacgtag
     4741 acgttgcacg ccctgcctcc ttgtggatgg aatgctcgcc aagtttctct tgagtggggt
     4801 ccatcgcgat gaaaaaccta gcattttcga tagtttgcgt gctttgtgcg ctgagcgcgc
     4861 agcaggccgc ggcgcagacg gccagcaact tctacgtgcg ggtcgatgcc ggcgggtctt
     4921 tcccgatcga cacgactctc gcgggcgagc agaacttctc gccctcggcg atggtcggcg
     4981 gcggcgtcgg cttcaagatt ctgcccttcc tgcgcaccga cgtcaccgcg tcctaccgca
     5041 ccgactacaa cgagaacgcg acggatccca acaccttcct ggggcagaag tcggagatca
     5101 agagcctggc cggcttcttc aatgggtatt tcgacattcc gacgggaccg ctgccgatca
     5161 agccctatat cggcgccgga gtcggggcag cgcgcaacca gacgggcacg acgtcgcagt
     5221 tcgatccttt caccggcaac acggcaagcg tcgacggcgc gacgaaaatg agcttcgcct
     5281 atcaggcgat gctgggcgtg gggttcccga tcttccccgg catcaccatc gatgccgcct
     5341 atcactatgt cgacctcggc cgcttcacca cggcaggggg tgtcagcttg aacggaacgc
     5401 ctggcgtgcc cctgaccggc cgcctcaagg ccaacgagat ccagctcggg ctgcgcgtcg
     5461 gcttctgagc gcgcggctca cggccccgga ctccgtgcaa ggccgcttgg cgtcgcgcgg
     5521 gcgacgacgc ttcgcgtcgc gccggggttg cggcgcggcc gctggcgccc gccgcgctcg
     5581 atgccctaat ctagccgccg caatcgagcg gtgaggcggg acgtggcgca gcacgacttc
     5641 gacatgttca cgatcggcgc gggctcgggc ggcgtcgcgt cgtcgcggcg cgccggctcc
     5701 tacggcgcgc gggtcgccat ctgcgagaac agccgcgtcg gcggcacctg cgtgatccgc
     5761 ggctgcgtgc cga
//
LOCUS       DASYNS010000177         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3939858, whole genome shotgun sequence.
ACCESSION   DASYNS010000177 DASYNS010000000
VERSION     DASYNS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3939858"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VGP50_05525"
     CDS             complement(<1..552)
                     /locus_tag="VGP50_05525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV8026866.1"
                     /translation="MRSRDEALEGSAVRLRRTGSGPPLVLLHCLGADHRLWEIAARGL
                     SQEFTLLTYDLPGHGEAPVPAGPYDIADLSTQLGTLLAREGIARAHLAGISLGGHVAQ
                     HFAATAPERVERLILIDTTPSHTEALSRNWRERAAAARAQGVASFVGALLRLWFNDTF
                     IAGDPPAVRYARETLERCPSEGYA"
     gene            1078..2166
                     /gene="torT"
                     /locus_tag="VGP50_05530"
     CDS             1078..2166
                     /gene="torT"
                     /locus_tag="VGP50_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HEV8026867.1"
                     /translation="MSRIWIAAAVVAAGALLVATAEIDVRAQGKTWYPFKVEEHNPPF
                     DMDGPKVEKEYTPLEKASTKWNICVSFPHMKDAYWLAVDYGVAAEAKDLGVTMHLVEA
                     GGYTNLSKQISQIEDCVANGAQAVVIGSISLDGLNKTVEELRAKKLPVIDVINGISSP
                     EISAKSLVSFKEMGYNAGAYIAKQHPKGSGKVQVAWFPGPPGAGWVEAGNKGFLDAVK
                     NSDVEVVETKYGDTGKEVQLKLVEDSLQAHPDIKYIAGTSPTTEAAVELLRERNLSEK
                     IKVVAYYFTPGVYQDIKRDRVMAAPTDSAVIQGRIAIDQAVRILEGKPYLKHVGPEIY
                     MIDKSNIGSFDYASSLAPENWSPVFNVE"
     gene            2172..>2712
                     /locus_tag="VGP50_05535"
     CDS             2172..>2712
                     /locus_tag="VGP50_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8026868.1"
                     /translation="MRSSGVAGPVVDTALESDTAGDGGAKSPALLRALGLSKHFPGVI
                     ALDAVDFDLRMGEVHVLFGENGAGKSTLIQTLAGVHRPSAGVIRVRDVAVELQSVHHA
                     RALGISAVFQEFSLVPQLTVEENLFLGAEITTHGLLNKRALRRRAQEILDRLGFALRP
                     GERVQYLSRAQQQMVEIAKA"
BASE COUNT          492 a          877 c          915 g          428 t
ORIGIN      
        1 cgcatagccc tcgctcgggc agcgctcgag cgtctctcgc gcatagcgca cggccggcgg
       61 atcgccggca atgaacgtgt cgttgaacca gaggcggagc agcgcgccga cgaaagaggc
      121 gacaccttgc gcgcgcgccg cagccgcgcg ctcccgccaa ttgcggctca gcgcctcggt
      181 atggctcggc gtggtgtcga tcaggatcag ccgctcgacg cgctcgggcg cggtcgcggc
      241 gaagtgctgc gccacgtgac cgcccagcga gatcccggcg agatgcgcgc gcgcgatgcc
      301 ttcgcgcgcg agcagggtgc cgagctgcgt cgagagatcg gcgatgtcat aaggacccgc
      361 cggcacgggc gcctcgccat ggccgggcaa gtcataggtg agcagcgtga attcctgaga
      421 gaggccgcgc gcggcgatct cccagaggcg atgatcggca ccgaggcaat gcaaaagcac
      481 gagtggaggg ccggagcccg ttcggcgcag cctgaccgcg ctgccctcga gggcctcgtc
      541 gcgagaacgc atatttttcc ttcgtttcgc gtcccggagt ccatcttctt gtaacacccg
      601 agactatcaa acgcccggtc atgcggtcga ggccgtctcc cgcacatttg cgcgacttcg
      661 atcgaaggga ggaagcgcct catccgcttg gcggatgacg cggcctcgcc cggatgctgg
      721 cgcaaaatga agaggcaccg cttcggggaa acgccgcgac gttgtgcgcg ttttccgcag
      781 ctgcatgaag ctcaggaacc tcggtcatgg tgacgcggcg ctgccggtgt cctcggcgtg
      841 atcggggcga catggcgatg ccgcgagcag gttccctgtt cttcaagatg ggacgagaac
      901 cggcggcgcg tccgcaccgc attgggtgcc gcgcgccgtc ggcgctgatt gacattgcaa
      961 aattgctggg ctgacaatga atcgacgcgg gggagcggca gccgacggtt gccgccaggc
     1021 gccgcggaag gggagggccg acagcggcct tcgcccgcaa ctaaagagag gcgcacgatg
     1081 tcgcgtatat ggattgctgc ggccgttgtc gctgcgggag cgctgctcgt cgccaccgcg
     1141 gagatcgacg tccgggccca aggcaagacc tggtatccct tcaaggtcga ggagcacaat
     1201 ccgcccttcg acatggacgg gcccaaggtc gagaaggaat acacgcccct cgagaaggcc
     1261 agcacgaagt ggaacatctg cgtgtccttc ccgcacatga aggacgcgta ctggctcgcc
     1321 gtcgattacg gcgtcgccgc ggaggccaag gatctcggcg tgaccatgca cctcgtcgag
     1381 gccggcggct acaccaacct cagcaagcag atctcgcaga tcgaggactg cgtcgccaac
     1441 ggagcgcagg cggtcgtgat cggctcgatc tcgctcgacg gcctcaacaa gacggtcgaa
     1501 gagctgcgcg ccaagaagct gccggtgatc gacgtgatca acggcatctc ctcgcccgag
     1561 atctccgcca agtcgctcgt ctcgttcaag gagatgggct acaacgccgg cgcctacatc
     1621 gccaagcagc atcccaaggg ctcgggcaag gtccaggtcg cctggttccc ggggccgccc
     1681 ggcgcagggt gggtcgaagc cggcaacaaa ggcttcctcg acgccgtcaa gaacagcgac
     1741 gtcgaggtgg tcgagactaa gtatggcgac accggcaagg aggtgcagct gaagctggtc
     1801 gaggattcgc tccaggcgca tcccgacatc aagtacatcg ccggcacgtc gcccaccacc
     1861 gaggccgcgg tcgagctttt gcgcgaacgc aacctttccg agaagatcaa ggtcgtcgcc
     1921 tactatttca cgcccggcgt ctaccaggac atcaagcgcg atcgggtgat ggcggcaccg
     1981 acggattccg cggtgatcca ggggcggatc gccatcgacc aggcggtgcg catcctcgaa
     2041 ggcaagccct acctcaagca tgtggggccc gagatctaca tgatcgacaa aagcaacatc
     2101 ggctcgttcg attatgcctc gtcgctcgcg ccggagaatt ggagcccggt cttcaacgtc
     2161 gagtgaggct ggtgaggtca tcgggggtcg ccgggccggt ggtggacaca gcattggaga
     2221 gcgacacggc cggtgacggc ggggccaagt cgccggcatt gctgcgcgcc ctgggtctga
     2281 gcaagcattt ccccggcgtc atcgcgctcg atgccgtcga tttcgatctg cgcatgggcg
     2341 aggtgcatgt gctgttcggc gagaacggcg ccggcaagtc aaccctgatc cagacgcttg
     2401 ccggcgtgca tcgtccgagc gcgggcgtca tccgcgtccg cgacgtcgcc gtcgagctgc
     2461 aatccgtgca tcacgcgcgc gcgctcggca tcagcgccgt gttccaggaa ttctctctgg
     2521 tgccgcagct caccgtcgaa gagaacctgt tcctcggcgc cgagatcacc acgcatgggc
     2581 tgctcaacaa gcgcgcgctg cgccggcgcg cccaggagat cctcgaccgg ctcggcttcg
     2641 cgctgcggcc gggcgagcgc gtccagtacc tgtcgcgcgc ccagcagcag atggtggaga
     2701 tcgccaaggc ct
//
LOCUS       DASYNS010000178         4529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4876375, whole genome shotgun sequence.
ACCESSION   DASYNS010000178 DASYNS010000000
VERSION     DASYNS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4529
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4876375"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..888)
                     /gene="fliP"
                     /locus_tag="VGP50_05540"
     CDS             complement(139..888)
                     /gene="fliP"
                     /locus_tag="VGP50_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944807.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HEV8026869.1"
                     /translation="MSARLWRLVALLLALTVLSAAPALAQTVTLDLGGEGGGSTTGHI
                     IELVALLTVLSLAPAILVTVTSFTRIVVVLSFLRNALGLQQTPPNIVLISLALFLTGF
                     IMAPTLEKSYNDGIAPLIADTMPQAEALALAAAPLHDFMTVHVRGHDLELFAGIAKLD
                     AAIEAKDVPFRVLVPAFMISELRRAFEIGFLLFLPFLVIDIVVSSVLMSMGMMMLPPV
                     TISLPFKLIFFVLVDGWYLVAGSLVQSFGPG"
     gene            complement(885..1181)
                     /locus_tag="VGP50_05545"
     CDS             complement(885..1181)
                     /locus_tag="VGP50_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HEV8026870.1"
                     /translation="MSFDTYSRFILALAFVVLLIGGCAWLARRFGLAGRFVVTGTKRR
                     LAIIEVLPLDGKHRLVLLRRDEAEHLVLLGQHTDLVIERGGGSGFSTMIDGGAS"
     gene            complement(1178..1471)
                     /locus_tag="VGP50_05550"
     CDS             complement(1178..1471)
                     /locus_tag="VGP50_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026871.1"
                     /translation="MTVATPQMALAEILDAVAGARHAVAEGAQVELLGLDGAVTRLCD
                     AARDLSPAERSPFADRLKELARSLDLLADDLARQDGAGLRHRAQSAYGSKDGG"
     gene            complement(1468..1767)
                     /locus_tag="VGP50_05555"
     CDS             complement(1468..1767)
                     /locus_tag="VGP50_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEV8026872.1"
                     /translation="MSRPPGERPEPVAVALQYDRKEDPAPRVVASGRGPIAERILELA
                     VAHGITVRQDADLAEILTAIEIGHQIPIAAFAAVAEILFHIYRANERQAVHRAAP"
     gene            1892..2800
                     /locus_tag="VGP50_05560"
     CDS             1892..2800
                     /locus_tag="VGP50_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEV8026873.1"
                     /translation="MDEIILHHYERSPFSEKVRLAFGLKGVSWRSVIIPRWMPKPDLM
                     PLTGGYRKTPVMQIGADIYCDTRLILREIDRRFPKPGLFTAGGADLIAAWADSTLFAN
                     TVGVVIGTFADAFPPEFKEDRIKFSGGAFDAERMKAHQPAIRAQFRAHLRWIEETFAD
                     GREYVMGPAPSIADFALYHTLWFVRGNVKEPDLLEGAGATKAWQERMARFGHGTPTPL
                     DPKAALAIAREANPKAVAPSRAADPSGRKPGEKVIVAANDTGRDPIAGELLAIDDQTI
                     VIRRRHADLGELHQHFPRAGFDLTAG"
     gene            2936..3334
                     /locus_tag="VGP50_05565"
     CDS             2936..3334
                     /locus_tag="VGP50_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HEV8026874.1"
                     /translation="MDLSQIPLFDAISKRLSWLGERQSVLAQNIANADTPGYVAMDVK
                     EPNFAGLVAGAGTHLPLLRTDPADIVPVGHFEQVAQPVTESAPNGNAVQLEDQMMKIS
                     NNASDHALTISLYKKQLALVKMALGQSPTG"
     gene            3349..3756
                     /gene="flgC"
                     /locus_tag="VGP50_05570"
     CDS             3349..3756
                     /gene="flgC"
                     /locus_tag="VGP50_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HEV8026875.1"
                     /translation="MDLEQSLQISFAGMAAQGTRLRVVSENLANADSTAQTPGGEPYR
                     RKVVTFKSVLDKSIGAMSVKVDRIAPGSGEFERRYDPSHPAADAEGYVLMPNVNPIAE
                     LLDMREAQHSYQANLDAIDAAKQMISRTINLLS"
     gene            3781..4095
                     /locus_tag="VGP50_05575"
     CDS             3781..4095
                     /locus_tag="VGP50_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HEV8026876.1"
                     /translation="MAAPISNAIAAYANAGRIGGAGAGMAPGAEASGGGFGDMLAQAA
                     GDLTNTLHQGEAASLQAVTGKADINQVVMAVTNAEVTLQAVIAVRDRVIQAYQDISKL
                     AI"
     gene            4109..4372
                     /gene="fliQ"
                     /locus_tag="VGP50_05580"
     CDS             4109..4372
                     /gene="fliQ"
                     /locus_tag="VGP50_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HEV8026877.1"
                     /translation="MNDADAIEIAHQTIVVTLKLGGPIMLLALVVGLAIAFLQALTQI
                     QEMTLTFVPKLVVILVSILVLLPFMLSTLSQFTESLMSRIAAG"
BASE COUNT          823 a         1497 c         1510 g          699 t
ORIGIN      
        1 cgcaaaccga gcctcactct cgtcccttct tcgaagaccc acctcagctc ccccgccaaa
       61 atgaatctct caaacctacc agagccttgg tacactcacc ctgcggcgtt ctgactcacc
      121 ctttctgact cagtaagact aacccgggcc gaagctctgc accaggctgc cggccaccag
      181 gtaccaaccg tcgaccagga cgaagaagat gagcttgaag gggagcgaga tggtcaccgg
      241 cggcagcatc atcatgccca tcgacatgag aacggacgag acgacgatgt cgatgacgag
      301 gaagggtagg aacagcagga agccgatctc gaaggcgcgg cgcagctcgc tgatcatgaa
      361 ggcgggcacg agcacgcgga atgggacgtc cttggcctcg attgcggcgt cgagcttggc
      421 gataccggcg aagagctcga gatcgtggcc gcgcacatgc accgtcatga agtcgtggag
      481 cggcgcggcg gcgagggcca gcgcctccgc ctgcggcatc gtgtcggcga tgagcggcgc
      541 gatgccgtcg ttgtaggact tctcgagcgt cggcgccatg atgaagccgg tgaggaagag
      601 cgcgaggctg atcagcacga tgttgggcgg cgtctgctgc agtcccagcg cattgcgcag
      661 gaaggacagc accacgacga tccgcgtgaa ggatgtgacg gtgacgagaa tggccggcgc
      721 gaggctcagc accgtcaaca gcgccaccag ctcgatgatg tggccggtgg tcgagccgcc
      781 accctcgccg ccgagatcga gcgtcaccgt ctgcgccagc gccggcgccg ccgagagaac
      841 ggtcagcgcg agaaggagcg ccacgagccg ccacagacgc gcgctcatga tgcgccgcca
      901 tcgatcatgg tcgagaagcc gctgccgccg ccgcgctcga tgacgaggtc cgtgtgctgg
      961 ccgagcagca cgagatgctc ggcctcgtcg cggcgcagca gcacgaggcg atgcttgccg
     1021 tcgagcggca gcacctcgat gatggcgagg cgccgcttgg ttccggtcac gacgaagcgg
     1081 cccgcgagac cgaagcggcg cgcgagccag gcacacccgc cgatgagcag cacgacgaag
     1141 gcgagcgcca ggatgaagcg cgagtacgtg tcgaagctca cccgccgtcc ttgctgccat
     1201 aggcgctctg agcgcgatgc cgcaagccgg ccccatcctg ccgcgcgagg tcgtcggcga
     1261 gcaggtcgag ggaccgggcg agctctttga gccggtccgc gaaagggctg cgctcggcgg
     1321 gcgagaggtc gcgcgcggcg tcgcagaggc gcgtcacggc gccgtcgagc cccagaagct
     1381 cgacttgcgc gccctcggcg acggcgtgcc gggcgccggc gacggcgtcg aggatctcgg
     1441 caagcgccat ctgcggcgta gccacggtca tggcgctgcc cgatgcacgg cttgccgctc
     1501 gttggcacgg tagatgtgga agaggatctc ggcgacggcg gcgaaggcgg cgatggggat
     1561 ctggtggccg atctcgatgg cggtcaggat ctcggcaagg tctgcgtcct ggcgcacggt
     1621 gatgccatgg gcgacggcga gctcgaggat gcgctcggcg atgggccccc ggccgctcgc
     1681 caccacgcgc ggtgccggat cctccttgcg gtcgtattgg agggcgacgg cgacgggctc
     1741 gggccgctca ccgggcggac gggacatgcg cgatcctttc tggaccggac gaagcacaac
     1801 ccccagtatg tctttgttag ccttaattat tcgttgccat gccccgcttt ggtgtggcac
     1861 tcatggcggt agagcgaggg agagagagac catggacgag atcatcctgc atcactacga
     1921 gcggtcgccc ttctccgaaa aggtgcgcct ggcattcggg ctcaagggcg tctcctggcg
     1981 ctcggtcatc attccgcgct ggatgcccaa gcccgacctg atgccgctca ccggcggcta
     2041 ccgcaagacg ccggtgatgc agatcggcgc ggacatctat tgcgacaccc gcctcatcct
     2101 gcgcgagatc gaccggcgct tcccgaagcc gggcctcttc accgcgggcg gcgccgatct
     2161 catcgccgcc tgggcggaca gcacgctctt cgccaacacc gtcggggtcg tcatcggcac
     2221 gtttgccgac gccttcccgc cggagttcaa ggaggatcgg atcaagttca gcggcggcgc
     2281 cttcgatgcc gagcgcatga aagcgcatca gccggccatt cgcgcgcagt tccgagcgca
     2341 tctgcgctgg atcgaggaga ccttcgcgga tggccgcgaa tatgtgatgg gccccgcgcc
     2401 gtcgatcgcc gattttgcgc tttatcacac gctctggttc gtgcgcggca atgtgaagga
     2461 gcccgacctc ctggaagggg cgggagcgac caaggcctgg caagaacgga tggcgcgctt
     2521 cggccatggc acgccgacgc cgctcgaccc caaggcagcg ctcgccatcg cgcgcgaagc
     2581 caatcccaag gccgtggcgc ccagccgcgc ggccgatccg agcggccgca agcccggcga
     2641 gaaggtgatc gtcgccgcca acgacaccgg gcgcgatcct atcgccggcg agctgctggc
     2701 gatcgatgat cagacgatcg tcatccgccg ccgccacgcc gatctgggcg aactgcacca
     2761 gcatttcccc cgcgccgggt tcgatctgac ggccggctag ccttcgcata ttaagggttc
     2821 acggagaaca cggagaagag tcgcagaaga cgggcggtcg cgcgaagcgc gccattcctg
     2881 gcttctccgt gacttcactg cttattaagc agaattgcgc aagatcctgg atatcatgga
     2941 tctcagccaa attccgctgt tcgacgcgat ctccaagcgc ctctcgtggt tgggcgagcg
     3001 gcaatcggtg ctggcgcaga atatcgccaa tgccgatacg ccgggctacg tggccatgga
     3061 tgtgaaggag ccgaatttcg ccgggctggt ggcgggggcg ggaacgcatc tgccgctctt
     3121 gcgcacggac cccgcggata tcgtccccgt cggccatttc gagcaggtgg cgcaacccgt
     3181 gaccgagagc gcgcccaacg gcaacgcggt tcagctcgaa gaccagatga tgaagatctc
     3241 gaacaacgcc agcgaccacg cgctgaccat cagcctctac aagaagcagc tcgcgctcgt
     3301 gaagatggcg cttggccagt cgcccacggg ctgagcggga gaaggttcat ggatctcgag
     3361 caatcgctgc agatctcctt cgccggcatg gcggcgcaag ggacgcggct gcgtgtcgtc
     3421 tccgagaacc tggccaatgc cgattcgacg gcgcagacgc cgggcggcga gccttatcgg
     3481 cgcaaagtcg tcaccttcaa gagcgtgctc gacaagagca tcggcgcgat gagcgtcaaa
     3541 gtcgaccgca ttgcccccgg gagcggcgag ttcgagcggc gctacgaccc gagccatccc
     3601 gccgccgatg ccgagggcta tgtcctcatg cccaacgtca atccgatcgc cgagctcctc
     3661 gacatgcgcg aagcgcagca cagctaccag gccaatctcg acgccatcga cgcggcaaag
     3721 cagatgatca gccgcaccat caacctcctc agctgataaa gcgcgggcaa aaggatcacc
     3781 atggctgccc cgatctcgaa tgccatcgcc gcttacgcca atgccggccg cattggcggg
     3841 gcgggggcgg gcatggcgcc gggcgcggaa gcgagcggcg gcggcttcgg cgacatgctg
     3901 gcccaggcgg cgggcgatct taccaacacg ctgcatcagg gcgaggccgc gagcctgcag
     3961 gccgtgaccg gcaaggccga catcaatcag gtggtgatgg ccgtcaccaa cgcggaggtc
     4021 acgctccagg cggtgatcgc cgtgcgcgac cgcgtcatcc aggcctatca agacatctcg
     4081 aagctcgcga tctgacgccg gttctgccgt gaacgacgcc gacgccatag agatcgcgca
     4141 tcagacgatc gtcgtcacgc tcaagctcgg cgggccgatc atgctgctgg cgctcgtcgt
     4201 cggtctcgcc atcgccttcc tgcaggcgct gacccagatc caggagatga cgctgacctt
     4261 cgtgcccaag ctggtcgtca tcctcgtctc gatcctcgtt cttctgccct tcatgctgtc
     4321 gacgctcagt cagttcaccg aatcgctgat gagccgcatc gccgcggggt aggcctcatg
     4381 caacggggct gccatacccc cctccctgac cctccccctc aaggggggag gggatttgct
     4441 tgcgccgcag cgcgatcttc cccctccccc cctgaggggg agggtggggt gggggggtgc
     4501 aacgccccgg cgatcgcgcg gcgaagctc
//
LOCUS       DASYNS010000179         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1600836, whole genome shotgun sequence.
ACCESSION   DASYNS010000179 DASYNS010000000
VERSION     DASYNS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600836"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1398
                     /locus_tag="VGP50_05585"
     CDS             199..1398
                     /locus_tag="VGP50_05585"
                     /inference="COORDINATES: protein motif:HMM:NF003477.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HEV8026878.1"
                     /translation="MSAQTSNGASRPDAAPVHLSRPAPGIIAALFCGYLCVGLPLPVI
                     PLFVHDRLGFSNLVVGLVIGIQFLATVLTRGYAGRLTDRHGGKRSALQGAAVCALGGV
                     LYLAAAMPGLAPATSLAFVVLGRVAAGFGESQFVTGCVSWSIASVGPQRAGMSMSWTG
                     IAMFAALAIGAPIGMALYQSDGLGAATGACIVAPLFAAGVAWSQASYITPAGPRLPFT
                     KVIGQILREGVGLMLQGVGLSGLTAFASLYFAARNWDRAGLVMTAFGIGFIVVRVVLG
                     SLPDRMSGYRVAFWSLVVEAIGQAMIWGAPDEIVALAGALVTGFGCALVFPALGVEAL
                     KRVLPANRGSAMGGFVAFLDIAYGVSGPVAGVIASQFGYAAVYLFGAVCALCGAALAV
                     APRRRQT"
     gene            1456..>1924
                     /locus_tag="VGP50_05590"
     CDS             1456..>1924
                     /locus_tag="VGP50_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8026879.1"
                     /translation="MSRIFISGSSTGLGLMVADLLAGKGHRVVLHARNAERAEAARRE
                     LPEAEAIVVGDVATIAGAKDVAAQVNGLGHVDALIHNAAVGYREGHRVTADGLPHVFA
                     INTLSAYVLTALIGRPKRLVYLSSGMHHHADANLDDILWKKRRWNGSTAYAESK"
BASE COUNT          249 a          676 c          643 g          356 t
ORIGIN      
        1 tggccggcga gctccgcaag cgcgccggca agctgatcgg cgtcgatgtc gccaagctgc
       61 gcgcggaggt gaacgcgtcg cgcgatcatc tgctcgaggt gagcggctac cgcaccgacc
      121 tcttcgccac ctccgccgcg agcgctgcgg cctgatccgg aggcgccgcc ctctcaacca
      181 agtcaatgga ggctgcccgt gtcagcacag acgtcaaacg gcgcgagccg gccggacgcc
      241 gcgcctgttc atctctcccg tcccgcgccc gggatcatcg cggcgctgtt ctgcggctac
      301 ctttgcgtcg gcctgccgct gccggtgatt ccgctgttcg tccacgaccg gctgggcttc
      361 agcaacctcg tcgtcgggct ggtgatcggc atccagttcc tcgcgaccgt gctcacccgc
      421 ggttacgcgg gacggctgac cgatcgtcat ggcggcaaac gttcggctct tcagggcgcg
      481 gcggtctgcg cgctgggcgg agtgttatac ctcgctgcgg caatgccggg cctcgcaccg
      541 gcaacgagcc tcgccttcgt cgtgctcggc cgcgtggcgg ccggtttcgg cgagagccag
      601 ttcgtgaccg gctgtgtgtc ctggtcgatc gcctcggtcg gcccgcagcg cgccggcatg
      661 tcgatgtcct ggaccggcat cgccatgttc gcggcgctcg ccatcggcgc accgatcgga
      721 atggcgctct atcaaagcga tggcctgggg gccgcgacgg gcgcctgcat cgtcgcaccg
      781 cttttcgccg cgggcgttgc ctggtctcaa gcgtcctaca tcaccccggc gggcccgcgc
      841 ctgcccttca ccaaagtgat cggtcagatc ctgcgcgaag gcgtcggcct catgctgcaa
      901 ggcgttggcc tgtcgggtct gacggccttc gcctcgctct acttcgccgc gcggaactgg
      961 gaccgtgccg gtctggtcat gaccgcgttc ggcatcggct tcatcgtcgt ccgtgtggtg
     1021 ctcggctctc tgccggaccg gatgagcggc taccgggtgg cgttctggtc gctggtggtc
     1081 gaggcgatcg gtcaggcaat gatctggggc gcgcccgatg agatcgttgc cctcgctggt
     1141 gcgctggtca cgggctttgg ctgcgcgctg gttttccccg cactcggcgt cgaagccctc
     1201 aagcgcgtgc tgcccgccaa tcgcggcagc gcgatggggg gcttcgttgc cttcctcgac
     1261 atcgcctacg gcgtctccgg tcccgtcgcg ggcgtcatcg caagccagtt cggctacgcg
     1321 gccgtctacc tcttcggcgc cgtctgcgcg ctgtgcggcg cggcgcttgc ggtcgctccc
     1381 cgtcgccgcc aaacctgacc gtcaatggtc gcgccggcgg atcgggtccg tcggcgaaga
     1441 cgaaggagca tcatcatgtc acgcatcttt atttcgggtt cttccaccgg cctcggcctg
     1501 atggtggccg atctgctcgc cggcaaaggc caccgcgtcg tgcttcacgc gcgcaacgcc
     1561 gagcgcgctg aagcagcgcg ccgggaactg ccagaggccg aggcgatcgt ggtcggcgac
     1621 gtcgcgacca tcgcgggcgc aaaggacgtc gcggcccagg tcaatgggct tggccatgtc
     1681 gacgctttga tccacaacgc cgcggtcggc tatcgcgaag gccatcgcgt caccgccgac
     1741 ggtctgccgc acgtgttcgc gatcaacacg ctgtcggcct atgtcctgac cgcgctgatc
     1801 gggcggccca agcggctcgt ctatctgagc tccggcatgc accatcatgc cgacgccaat
     1861 ctcgacgaca tcctctggaa gaagcgccgc tggaacggtt cgaccgccta cgcggagagc
     1921 aagc
//
LOCUS       DASYNS010000180         4371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_350713, whole genome shotgun sequence.
ACCESSION   DASYNS010000180 DASYNS010000000
VERSION     DASYNS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350713"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="VGP50_05595"
     CDS             <1..613
                     /locus_tag="VGP50_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEV8026880.1"
                     /translation="AGASGRQPVAITGEAVRERVHLLRAQSDAIMVGIGTALADDPML
                     TCRLPGMAGYSPERIVLDSALRLPLASRLVRSARETPLWVVAGAAAVPRAGEALQAQG
                     VEIFRAEGSEGHLDLADVLKSIAARGITRLMVEGGPTLAATFVAADLVDVVYLFYSSK
                     RVGPGGIDALEGLPLTTLTQSPWLRRITSEPVGADTLTVLERR"
     gene            600..1241
                     /locus_tag="VGP50_05600"
     CDS             600..1241
                     /locus_tag="VGP50_05600"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV8026881.1"
                     /translation="MSEGEAMFTGIVNDLGEVLQVAEKAEGLRRLTVKCGYDPDTIDI
                     GASIACSGICLTVVARGRTRQRRFFAVDAAAETLRVTTAGAWQRGTMLNLERPLRVGD
                     ELGGHMVSGHVDGVAELIAREDLPDMARLFLQAPAELARFIAPKGSVALDGVSLTVNE
                     VKGDRFSVLIIPHTLKVTTFGELGVGARMNIEVDLMARYAARLLQTPQSSALR"
     gene            1260..2096
                     /locus_tag="VGP50_05605"
     CDS             1260..2096
                     /locus_tag="VGP50_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8026882.1"
                     /translation="MRELSGKTAFVTGGASGIGLALGRAFAEAGMNVMLADVEIEALT
                     AAVKSLHNFGPDVRGTTCDVTDPDSVERAAEASYKAFGHVHVVCNNAGVAAGGGVDDI
                     SLDDWRWVLEVNLMGVLHGVRTFLPHFRAHGEGGHFVNTASMAGMLSGLGFSPYAASK
                     FAVVTMSEGLAMQLKPLGIGVSVLCPGWVRTRINASGRNRPERYGPTRTPDPDSPAAA
                     LAAYIDESLRSGLDPAEVAARVLTAIREDELYVFTHPQMRAAMEERFAAISAAMDRAA
                     AR"
     gene            2170..2658
                     /gene="ribH"
                     /locus_tag="VGP50_05610"
     CDS             2170..2658
                     /gene="ribH"
                     /locus_tag="VGP50_05610"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV8026883.1"
                     /translation="MAGPRQGKREKGDGKGTRILLVEARFYDDIADALLAGAMKALTE
                     AQADVERISVPGSLEIPGAIAIALDAAQKKRRPYDGVVALGCVIRGDTIHFDIVSHQS
                     ARGLMDLSIARSLPVGNGIITVDNKAQALARARMEEQDKGGDAARAALTLVGLKRRLA
                     ER"
     gene            2655..3164
                     /gene="nusB"
                     /locus_tag="VGP50_05615"
     CDS             2655..3164
                     /gene="nusB"
                     /locus_tag="VGP50_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV8026884.1"
                     /translation="MKMVRAAEKSGAEEPRKANKRGAARLAAVQALYQMDLAGTGVNE
                     IFAEFESHWLGGEVEGAQYRPAEAAFFRDVVGGVVREQNSLDPQIDAALAHGWPLKRI
                     EALLRAVLRAGAYELAFRRDIPARVVTSEYVDVAGSFVDQEETGMVNAVLDQLARQLR
                     AAEFGSASG"
     gene            complement(3169..>4371)
                     /locus_tag="VGP50_05620"
     CDS             complement(3169..>4371)
                     /locus_tag="VGP50_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV8026885.1"
                     /translation="ASCVAAPRPRGNAVEPFDLYSPAIDADPFPYYAELRDKYPCYWS
                     EGARLWILSRYGDIVEAARDWQTFSSSQGNMIDELPGRAGATLGTTDPPRHDRLRALS
                     QAAFLRRNLDRLVEPTLEIADRALERIAAQGTFEFIDDFSSRITVGLLFRTMGLPERD
                     HAEIRRKAILAISTDKAAKGRTPEHIAAFQELSDFLTEEVAVRRANPTDDLITHLAEA
                     EIDGDRLSEREIVLTTATFVMAGVESLSSFMSVFALNLHDYPDARRRLVADPVLVAPA
                     IEESLRFNTSAQRFKRVVMRETTLHGQTLRAGDKIALAFGSGNRDERKFPDPDKFDID
                     RRPQGHLGFGSGKHFCLGSQMARLVTEVAMRRFLERVPDYHLAVDKVAWNSSSNFRSP
                     TALPFACG"
BASE COUNT          697 a         1431 c         1522 g          721 t
ORIGIN      
        1 agccggcgcg tccgggcggc agccggtcgc cattaccggc gaggcggtgc gcgagcgggt
       61 ccatctgctg cgcgcacaga gcgacgcgat catggtcggc atcggtaccg cgctcgccga
      121 cgacccgatg ctgacctgcc gcctgcccgg gatggctgga tattcgcctg agcgcatcgt
      181 gctcgacagc gcgcttcgcc tgccgctcgc gtcgcgcctg gtgcggagcg cgcgggaaac
      241 gccgctgtgg gtggtcgccg gggcagcggc ggtgccacgc gccggggagg ccttgcaggc
      301 gcagggcgtc gaaatctttc gcgcggaagg gagcgagggc catctcgacc tcgcggacgt
      361 gctcaaatcg attgccgcgc gcggcatcac ccggctcatg gtcgaaggcg gcccgaccct
      421 tgctgccacc ttcgtcgccg cggacctggt cgatgtggtg tacctgttct attcgtcgaa
      481 gcgcgtcggg cctggcggca tcgatgcgct cgagggactg ccgctcacta ccctgacgca
      541 gtcgccgtgg ctgcgacgca tcacgagcga gcccgtcggg gccgacacgc tcaccgttct
      601 tgagcgaagg tgaggccatg ttcaccggca tcgtcaatga tttgggcgag gtccttcaag
      661 tcgccgagaa ggccgagggt ttgcgccgcc tgaccgtcaa atgcggctac gacccggaca
      721 cgatcgacat cggcgcctcc atcgcctgct ccggcatctg cttgacggtc gtggcgcgcg
      781 ggcggacacg tcagcggcgg ttctttgccg tcgatgccgc cgcggaaacg ctgcgcgtga
      841 ccaccgccgg ggcctggcaa cgcgggacga tgctcaacct cgaacggccg cttcgcgtcg
      901 gcgacgagct cggcggccac atggtcagcg gccatgtcga tggcgtcgcg gaacttatcg
      961 cgcgggagga tttgcccgat atggcgcggc tctttttgca ggctccggcc gagcttgcgc
     1021 ggttcatcgc gcccaaaggc tccgtcgcgc ttgacggtgt ctcgctcacc gtcaacgagg
     1081 tgaaaggcga ccggttttcc gtgctgatca ttccgcacac gctcaaggtc acgacgttcg
     1141 gcgagcttgg cgtcggtgcg cgcatgaaca ttgaagtcga ccttatggcg cgctacgccg
     1201 cacggctctt gcagacgccg caatcgagcg cgttgcgctg aggagcatcg gaggcaatca
     1261 tgcgggagct ttcgggcaag acggcgttcg tgaccggcgg ggcgagcggc atcgggctcg
     1321 cgctcggccg ggcgtttgcc gaggcgggca tgaatgtgat gctcgccgac gtcgagatcg
     1381 aggccctgac ggccgcggtc aagagcctgc acaattttgg accggacgtg cgcggcacga
     1441 cctgtgacgt gaccgatccc gacagcgtcg agcgtgcggc cgaggcgtcc tacaaggcct
     1501 tcggccatgt ccacgtggtc tgcaacaatg ccggcgtcgc cgccggcggc ggcgttgacg
     1561 acatttcgct cgacgattgg cggtgggtgc tcgaggtcaa tctgatgggc gtcctgcacg
     1621 gcgtccgcac gttcctgccg cacttccgcg cccatggcga gggcggtcac ttcgtcaaca
     1681 ccgcctccat ggccggcatg ctcagcgggc tcgggttcag tccgtacgcg gcgagcaagt
     1741 tcgcggtggt caccatgtcg gaggggctgg cgatgcagct taagccgctc ggcatcggcg
     1801 tgagcgtgct gtgtcccggt tgggttcgca cgcgcatcaa cgccagcgga cgcaaccggc
     1861 cggagcgcta cggcccaacc cggacgccgg acccggatag cccggcggcc gcgcttgcgg
     1921 cttacatcga cgaaagtctg cggtcagggc tcgaccccgc tgaggtcgca gcgcgcgtcc
     1981 tcactgcgat ccgcgaggac gaactctacg tgttcacgca tccgcagatg cgcgccgcga
     2041 tggaagagcg gttcgccgcc atctccgcgg cgatggacag agcggcggcc cgctagcgga
     2101 cacggttgac gcggcttgtt cgcgcttccc gcgcttgcta cgaccgcgcc aattcggagt
     2161 ggaacgacga tggccggtcc gcggcaaggc aagcgcgaga agggcgacgg caagggaacg
     2221 cgcatcctcc tcgtcgaggc gcgcttctac gatgacatcg cggatgcgct cctcgccggt
     2281 gccatgaagg cgcttaccga ggcgcaagcc gatgtcgagc gcatcagcgt gccgggctcg
     2341 ctggaaattc ccggcgccat cgccatcgcc ctcgacgcgg cgcaaaagaa gcgccgcccc
     2401 tatgacgggg tcgtcgccct cggctgcgtg atccgcggcg acaccatcca cttcgacatc
     2461 gtctcgcatc agtccgcgcg cggcttgatg gatctctcga tcgcccgcag cctgccggtc
     2521 ggcaacggca tcatcaccgt cgataataag gcgcaggccc tcgcgcgcgc ccgcatggag
     2581 gagcaggaca agggcggcga cgcggcgcgg gctgcgctta cgctggtcgg cctcaagcgc
     2641 cggctcgcgg agcgatgaag atggttcgcg ccgccgaaaa atcgggagcc gaggagccgc
     2701 gcaaggccaa caagcgcggc gccgcccgcc ttgccgccgt gcaggcgctc tatcagatgg
     2761 acctcgccgg taccggcgtg aacgagatct ttgccgagtt cgaaagccac tggctcggcg
     2821 gcgaggtcga gggcgcgcag tatcggccgg cggaagcggc cttcttccgc gatgtcgtcg
     2881 gcggtgtcgt gcgtgagcaa aatagccttg atccgcagat cgacgcggcg ctggcgcacg
     2941 gctggccgct caagcgcatc gaggcgctgc tgcgcgcggt gctgcgcgcc ggcgcctacg
     3001 agcttgcctt tcggcgcgac ataccggcgc gcgtggtcac ctcggaatat gtcgacgtgg
     3061 ccggttcctt cgtcgatcaa gaggagaccg gcatggtcaa tgccgtgctc gaccagcttg
     3121 cgcgccagct gcgcgccgcc gaattcggca gcgcgtccgg ctgacccatc acccgcaggc
     3181 gaagggcagc gcagtcgggc tgcggaagtt cgacgaggaa ttccaggcga ccttgtccac
     3241 cgcgaggtga tagtcgggca cgcgctcgag gaagcggcgc atggccactt cggtgaccag
     3301 ccgcgccatc tggctgccca ggcagaaatg cttgccggag ccgaagccga ggtgcccctg
     3361 cggacggcgg tcgatgtcaa acttgtcggg gtcgggaaac ttgcgctcat cgcgatttcc
     3421 cgagccgaaa gccaacgcga tcttgtcgcc ggcgcgcagc gtctggccgt gcagagttgt
     3481 ctcgcgcatg actacgcgct tgaagcgctg cgccgaggtg ttgaagcgca gcgactcctc
     3541 gatcgccggc gcgaccagca ccggatcggc cacaagacgc cgccgcgcgt ccgggtagtc
     3601 gtgaagattg agagcgaaca cgctcatgaa gctcgacagc gactcgacgc cggccatgac
     3661 gaaggtcgcc gtggtgagca cgatctcgcg ttccgagagc cggtcaccgt cgatctcggc
     3721 ttcggcgaga tgcgtgatca ggtcgtcggt cggattggcg cgccgcaccg ccacctcttc
     3781 ggtcaggaaa tcgctcagtt cctggaatgc ggcgatgtgc tccggcgtgc ggcccttcgc
     3841 cgctttgtcg gtcgagatgg caagaatcgc cttgcgccgg atttcggcat ggtcgcgctc
     3901 cggcagaccc atggtgcgga acaagagccc gaccgtgatc cggctggaga aatcgtcgat
     3961 gaactcgaag gtaccttgcg ccgcgatgcg ctcgagcgcc cggtcggcga tttccaaggt
     4021 cggctcgacc agccggtcga gattgcgcct cagaaatgcc gcctggctca aggcgcgcag
     4081 ccgatcatgg cgcggcgggt cggtcgttcc gagcgtcgcg ccggcgcggc ccggaagctc
     4141 gtcgatcata ttgccttggg aggaggagaa ggtctgccag tcgcgcgcgg cttcgacgat
     4201 atcgccgtag cgggacagaa tccagagccg cgcgccctcg ctccaatagc agggatattt
     4261 gtcgcgcagc tcggcgtagt agggaaacgg atccgcatcg attgcgggcg agtagaggtc
     4321 gaaaggctcc acggcatttc ctcgcggtcg tggcgctgct acacaactgg c
//
LOCUS       DASYNS010000181         5205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1911182, whole genome shotgun sequence.
ACCESSION   DASYNS010000181 DASYNS010000000
VERSION     DASYNS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911182"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VGP50_05625"
     CDS             complement(<1..242)
                     /locus_tag="VGP50_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026886.1"
                     /translation="MRRIIAAAIAAMVLGAGSAAAQGLDVLTHHNDIFRSGVYSAETI
                     LSPSSVSPPRFGKLFSRNIIGQVWGQPLYVHGVSIAG"
     gene            complement(319..1116)
                     /locus_tag="VGP50_05630"
     CDS             complement(319..1116)
                     /locus_tag="VGP50_05630"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEV8026887.1"
                     /translation="MPALHEMQAAFRRALIEGDAAALVALVEADGIGASKRVAIYRNN
                     VACALTDALRDTFPAVCRLVDERFFAYAADTFIQRHPPQRACLAEYGARFPDFLADFP
                     PCRELVYLPDVARLEWLMNVAAHAEDADALAASALAGIAGDEAPRLIFRLAPAIGLLK
                     SPWPVDRIWRANRPETGQEETIDLGEGGVRLEIGRHDGAVVLRHLDAASFVFRDAIAR
                     GATLAAATRAVLTVDGRFDLEAALAALFAESAVVASSVAPEAAHASL"
     gene            complement(1100..1963)
                     /locus_tag="VGP50_05635"
     CDS             complement(1100..1963)
                     /locus_tag="VGP50_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEV8026888.1"
                     /translation="MTGEQTGKSDAAIGVGLRGPHMAEIIATRPAIGWFEVHAENYMG
                     GGPALSRLDEIRRDYPLSLHGVGLSLGTAEGLDREHLRRLKALIDRTEPMLVSEHLAW
                     SVSGGVYLADLLPLPLTAEALAVVADNVALAQEVLRRRLLVENPSSYLRFRHGTMSEP
                     DFLAELVRRTGCGLLCDVNNIHVSCANLGADADAYLDALPAGAVGEIHLAGHSAVLRS
                     GHTLLIDDHAAPVSPEVWRLYRRALGRFGRVPSLVEWDKALPPLCGLLAEARAAERIA
                     RAEEDGDACAA"
     gene            complement(1991..2275)
                     /locus_tag="VGP50_05640"
     CDS             complement(1991..2275)
                     /locus_tag="VGP50_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HEV8026889.1"
                     /translation="MQQNTTRIAFLVASAIGATVALPMLAKAGPAPAPSFQAEKCYGI
                     NAAGKNDCASSNSHSCAGEAKRASDPKSWIFVPTGTCQKIQGGSLTPKET"
     gene            2593..3060
                     /locus_tag="VGP50_05645"
     CDS             2593..3060
                     /locus_tag="VGP50_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV8026890.1"
                     /translation="MTDRITAVAAAPGWRAPALALIVRLERFPLSLLQLLFRLSIAAV
                     FWNSGLTKIASWQTTVVLFRDEYHVPLLPPELAAALAATVELSCPVLLVLGLATRLAT
                     LPMLGMTFVIEAFVYPEDWIEHLTWASFLLFILTRGPGMLSLDHLIRRRLLGD"
     gene            3205..4386
                     /locus_tag="VGP50_05650"
     CDS             3205..4386
                     /locus_tag="VGP50_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV8026891.1"
                     /translation="MDSAADAGAGRQTKRAKAQAPQKAPVPPKSSKTPIAQNVLVFQG
                     GGALGAYQAGVYHALSEGGIEADWVIGTSIGAINGALIAGNEPKNRLARLREFWDLIS
                     TRPVEQPWSPAYFAALFSPGASQVLQGVPNFYEANRHANWGLNASVGVERAAFYGTEP
                     LRRTLTGLVDCNYLNAHHTRLTVGAVNVRTAEMRYFDSRDMPLGVDHIMASGAIPPAF
                     PAIRIDGETYWDGGLYSNTPMEAVLDDKPRRNSLIFSVNMWQPRAEEPGSIWDVLNRQ
                     KDIQFASRAQSHVARQEQLHRLRHIVRELGMRLPPEQREDPAVRELTSYGCATTMHLV
                     LLLLPRAADGKSKDIDFTRAGVRERWQTGYEHARRVLASKPWEGEVGALDGIVIHDAT
                     Q"
     gene            complement(4532..5131)
                     /locus_tag="VGP50_05655"
     CDS             complement(4532..5131)
                     /locus_tag="VGP50_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026892.1"
                     /translation="MRRAFRESVIKKVLAMTRRRLLFSPLPLGTLAGLFVLFSLPPAS
                     ARSVSDYCRLFTKEDIDKALNTHITEVDPGPMKVVLGAEGLHDQACVFIAGSRLLRIT
                     ITETRSTEDAQRLYKKAVSGQSAVNAADAKPLAGIHDEAVLAGNTVEMRRQNLVVDFS
                     LRDFVADDESGLALARALAAKVSQRISGAPARRHKRASQ"
BASE COUNT          886 a         1716 c         1755 g          848 t
ORIGIN      
        1 ccggcgatcg agacgccgtg gacatagagc ggctgtcccc agacctggcc gatgatgttg
       61 cgcgagaaga gcttgccgaa gcgcggcggg ctcaccgagc tcgggctcag aatggtctcg
      121 gcgctgtaga cgccgctgcg aaagatgtcg ttgtgatgag tgaggacatc gaggccctga
      181 gccgcggccg agccggcgcc caacaccatc gccgcgatgg cggccgcgat gatcctgcgc
      241 atgcttttcc ccctcccgac gctggtcggt gaggggccat taaacaccgg atcggcagag
      301 cttgcgatgt gacttgcgtc acagcgaagc gtgagcggcc tccggcgcaa cgctcgaggc
      361 gacgacggca ctctcggcga agagcgcggc aagcgccgcc tcgagatcga agcgtccatc
      421 gaccgtcagc accgcgcggg tcgcggcggc cagcgtcgca ccgcgcgcga tggcgtcgcg
      481 gaacacgaag ctcgcggcgt cgaggtggcg aaggaccacc gcgccgtcgt ggcggccgat
      541 ctcgaggcgg acaccgccct cgccgagatc gatcgtctcc tcctgcccgg tctccggccg
      601 gttcgcgcgc cagatgcgat cgacgggcca gggcgacttg agaagaccga tggcgggggc
      661 aaggcggaag atgagccgcg gcgcctcatc gccggcgatg ccggccagcg ccgatgcggc
      721 aagggcgtcc gcgtcctcgg cgtgagcggc gacattcatg agccattcga gccgcgcgac
      781 atccgggaga tagacgagct cccggcaggg cggaaaatcg gcgagaaaat cgggaaagcg
      841 cgcgccgtat tcggcgaggc aggcacgctg cggcgggtgg cgctggatga aggtgtcagc
      901 tgcatatgcg aagaaccgct cgtccacgag gcggcacacc gccggaaaag tgtcgcgcag
      961 cgcgtccgtc agcgcgcagg cgacgttatt gcggtagatc gcgacccgct tgcttgcgcc
     1021 gatgccgtct gcctcgacga gcgcgacgag cgccgccgcg tcgccctcga tgagcgcgcg
     1081 gcggaaagcc gcctgcattt catgcagcgc aggcatcgcc gtcttcctct gcgcgcgcga
     1141 tgcgctccgc cgcgcgcgct tcggcgagca atccgcatag cggcggcagc gccttgtccc
     1201 attcgacgag gcttggcacg cggccgaagc gcccaagcgc gcggcgatag aggcgccaga
     1261 cctcgggcga gaccggcgcc gcgtgatcgt cgatcagcag cgtatggccg gagcggagca
     1321 cagcgctgtg tcccgccaga tggatctcgc cgaccgcgcc ggccggcagt gcgtccagat
     1381 acgcgtccgc gtcggcgccc agattggcgc agctgacgtg gatattgttg acgtcgcaga
     1441 ggagcccgca gccggtgcgt cgcacgagct cggcgaggaa gtcgggctcg ctcatcgtcc
     1501 catgacggaa acgcagatag gaggacgggt tctcgaccag gagccggcgg cgcagcacct
     1561 cctgcgccaa ggcgacgttg tcagcgacga cggccagcgc ctccgccgtc aaaggcaacg
     1621 gcagcaagtc ggcgagatag acgccaccgc tcacgctcca ggccaggtgc tcggaaacca
     1681 gcatcggctc cgtccgatcg atgagcgcct tcaagcgacg cagatgctcg cgatcgagtc
     1741 cttccgcagt gccgagagag aggccgacgc cgtggaggct cagcgggtag tcgcgccgga
     1801 tctcgtcgag acgcgacagc gccgggccgc cgcccatgta gttctccgca tggacctcga
     1861 accagccgat cgcgggacgg gtggcgatga tctcggccat atgcggaccg cgcaagccga
     1921 cgccgattgc cgcgtcgctc ttgccggtct gctcgccggt catcgccacc cctcccgcgc
     1981 gtcgcaagcc tcaggtttcc ttgggcgtga ggctgccgcc ctggatcttc tggcaggtgc
     2041 cggtcggcac aaagatccac gacttcggat cgctcgcgcg cttcgcctcg ccggcgcagg
     2101 aatggctgtt cgaagacgcg cagtcattct tgcccgcggc attgatgccg tagcatttct
     2161 cggcctggaa gctcggcgct ggcgccggcc ccgccttcgc cagcatgggc agcgcgaccg
     2221 tcgcgccgat ggcagaggcg acgagaaagg cgatcctggt ggtgttctgc tgcatggaag
     2281 ggactcctct tgtgcttgat gttgcaacgc cgatccggca gccgccggcg gtgtgcacgt
     2341 cctctgttcg cgctcgggtg gccatggtta cgctctcccg cggcgatttc catccgatgc
     2401 cgcaagcggc cgatcacgat accgagaacg tcgtcgttct gctgtcatca ataagccagg
     2461 tgcatcgaaa ggatagacag acggcatttc cgctcggcag cggaacccgg cagtctcacg
     2521 catttgccga tatgccaaag tctcgccggc gatcgccgtc gctccggctt gtggccaatg
     2581 aggagagtcg agatgaccga tcgcatcacc gccgtcgctg ccgctccggg ctggcgcgct
     2641 ccggcgctcg ctctgatcgt gcggctggag cgctttccgc tcagcctgct tcagttgctg
     2701 ttccggctct ccatcgcggc ggtcttctgg aattccgggc tcaccaagat cgcgagctgg
     2761 caaaccaccg tcgtgctgtt ccgcgacgaa taccatgttc cgctgctgcc gccggagctg
     2821 gcggccgcac tcgcggccac ggtcgagttg agctgccccg tattgctggt cctcggcctg
     2881 gcaacccggc ttgccacttt gccgatgctc ggcatgacct tcgtcatcga ggccttcgtc
     2941 tatcccgagg actggatcga gcatctcacc tgggcttcgt ttctgctttt catcctgacg
     3001 cgcgggcccg gcatgctctc gctcgatcat ttgattcgtc gccggctgct cggcgactga
     3061 ccatatggct aggggcaatg gccagggtgg acgaggtcga ggcgctcgcg gtattcctgg
     3121 tcctggggag acggcgcttt gattgccgac gagatgtcgg caagcgagca ccgacgggcc
     3181 gcgcactcat aaagggagac tgtcatggac tcggcagcag atgctggagc tggtcggcaa
     3241 acgaagcgcg cgaaggcgca agcgccgcag aaggcgccag tgccgccgaa gagcagcaag
     3301 acaccgatcg cccagaatgt gctcgtcttt cagggtggcg gcgcgctcgg ggcctatcaa
     3361 gccggcgtct atcacgcgct gtcggaaggc ggcatcgagg ccgattgggt gatcggcacg
     3421 tcgatcggcg ccatcaacgg cgcgctcatc gccggcaatg agcccaagaa caggctggcg
     3481 cggctgcggg agttctggga cctcatcagc acccgccccg tcgagcagcc ctggtcgccg
     3541 gcgtatttcg ccgccctctt ctcccccggc gcctctcagg tgctgcaagg cgttcccaac
     3601 ttttacgagg ccaaccgcca tgcaaactgg ggcctcaacg catcggtggg cgtcgagcgc
     3661 gccgcattct acgggacgga gccgctgcgg cggaccctca ccgggctcgt tgattgcaac
     3721 tatctgaatg cgcaccacac gcgtctgacg gtcggcgcgg tcaacgtgcg caccgccgag
     3781 atgcgctact tcgacagccg cgacatgccg ctcggcgtcg accacatcat ggcgtcggga
     3841 gcgatcccgc cggcgtttcc tgccatccgc atcgacggcg agacctactg ggacggcggc
     3901 ctctactcca acacgccgat ggaggccgtg ctcgacgaca agccgcggcg caactcgctg
     3961 atcttctcgg tcaacatgtg gcagccgcgc gccgaggagc ccggcagcat ctgggacgtg
     4021 ctcaatcgcc agaaggacat tcaattcgcc agccgcgccc aaagccatgt cgcgcgccag
     4081 gagcagcttc atcggctccg ccacatcgtc cgcgagctcg ggatgcggct cccgccggag
     4141 cagcgcgagg atccggcagt gcgcgagctc acgagctatg gctgcgcgac aaccatgcat
     4201 ctcgtgcttc tgctgttgcc gcgcgccgcc gatggcaaaa gcaaggacat cgatttcacg
     4261 cgcgccggcg tccgcgagcg ctggcagacc ggctatgagc atgcccggcg cgtgctcgcc
     4321 agcaaacctt gggaaggcga ggtcggcgcg ctcgatggca tcgtcatcca cgacgcgacg
     4381 cagtaagcgg ggctggcgag gcggacatcg ccgccgcgag gaggagcctc tggggaggag
     4441 cgcctgcggg tcgcggccat gcagatgagc caggcgacgg cgcggctttg gaagcaatga
     4501 atactcgccg ccggcggcga gactgatcgc gctattgcga ggctcttttg tgacgacggg
     4561 ccggcgcgcc ggagatcctt tgcgaaacct tcgcggccaa tgcccgggca agcgcaagac
     4621 cgctctcgtc atccgcaacg aagtcacgca gcgagaagtc gacgaccaga ttctgacgcc
     4681 gcatctccac ggtattcccc gcaaggacgg cctcgtcatg aatgccggcc aggggcttcg
     4741 cgtcggcggc attcactgcc gactgaccgg acacggcttt cttgtagagg cgctgtgcgt
     4801 cctccgtcga ccgcgtctcc gtgatggtga tgcgcagcag gcgcgatcct gcgatgaaga
     4861 cgcaggcctg atcatgcaag ccctcggcgc cgaggaccac cttcatcggc cctggatcga
     4921 cttcggtgat atgcgtgttg agcgctttat cgatgtcctc tttcgtaaag agccgacaat
     4981 agtcgctgac cgagcgagca gaggccggcg gcagcgagaa taggacgaag aggccggcca
     5041 gggtgccgag cgggagcggg ctgaagagca ggcggcgtcg agtcatggca agcacttttt
     5101 tgatcacgct ttctctgaag gcgcgccgca tcgtcgatgt cttcgtaatc ggcacgcaag
     5161 cgcatggaaa gtgagcgtgc gccaatcagg attgggcggt cagcg
//
LOCUS       DASYNS010000182        11216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_40196, whole genome shotgun sequence.
ACCESSION   DASYNS010000182 DASYNS010000000
VERSION     DASYNS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11216
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40196"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2102
                     /locus_tag="VGP50_05660"
     CDS             <1..2102
                     /locus_tag="VGP50_05660"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEV8026893.1"
                     /translation="AADLMMGESRESAVAAQSIRVNVDVLENLMTMVSELVLTRNQLM
                     QILRTQKDTDFASPLQRLSHITTELQEGVMRTRMQPIGNAWAKLPRVLRDLSQELGKR
                     IDLQMFGADTELDRQVLEMVKDPLTHMVRNSADHGIESPEERLREGKPETGTITLNAY
                     HEGGHIIIEISDDGRGIDLERIKQKAVSVGLASESEVAAMTEQQIHQFIFRPGFSTAT
                     SVTNVSGRGVGMDVVRTNMEKIGGTVEMKSRENKGSTFVIKIPLTLAIVSALIVECGG
                     ERFAIPQISVVELVRAAAHADVSIEWLNNAPVLRLRDRLLPLVSLYELLKLGRPADRP
                     KETFIVVTQVGTHSFGIIVDRVYDTEEIVVKPVAPILRNIPMFSGNTILGDGSVIMIL
                     DPNGIAASAGEIAGNASGVRAEPVAARAVVRANLTSLLLFRVGGGAPKAVQLSLIARL
                     EEIDLAQIEYSNERPVVQYRGKLMPIVTIDQTAPSGEGRRPILVFADKDHIMGLVVDE
                     IVDIVEEELKIELTIDRPGLVGSAVIAGKATDVIDAGYYVNQTYSDWFGKPTLAMESQ
                     SQAESSEGGKRLLLVDDSPFFRNMLTPLLAAAGYAVTTADSGDHALQICASGEDFDII
                     VSDIEMPGMNGFEFASAVRSDSRWQSTPLVALSAHASQKDIDRGRAAGFSDYVPKFDR
                     EALLATLSEAINERGAA"
     gene            2105..2593
                     /locus_tag="VGP50_05665"
     CDS             2105..2593
                     /locus_tag="VGP50_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEV8026894.1"
                     /translation="MITIRSAPLDAAAASDEVHELVSVTVADQIFGIPVLQVQDVLGP
                     QRITRIPLAPPEVAGSLNLRGRIVTAIDLRTRLRLPPLPEARKSMSVVVDHEGELYSI
                     LVDGVGEVLKLNGNSAERNPPTLDATWRDVSAGVYRLEKTILIVLNVERVLDFSARAD
                     AI"
     gene            2608..3006
                     /locus_tag="VGP50_05670"
     CDS             2608..3006
                     /locus_tag="VGP50_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8026895.1"
                     /translation="MQSAQARSKGQKKFCLIVDDSRVVRKVARRILEDLSFAIEEATD
                     GKTAMEACLRKMPDVVLLDWNMPVMSGIDFLRQLRRTKGGDVPVVVFCTTENDVHHIK
                     EALGAGANEYIMKPFDSDILEAKFAQVGLL"
     gene            3069..4202
                     /locus_tag="VGP50_05675"
     CDS             3069..4202
                     /locus_tag="VGP50_05675"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEV8026896.1"
                     /translation="MVVDDSAIVRGLLIRSLEADPEVTVVASASNGEIALAALGRHDV
                     EVVILDIEMPVMDGLTALPKMLAAKPGLKVLIASSLTRRGAEISLRALAAGAADYLTK
                     PGTSALTSADEFKHELLVKVKALAQAGRSALGITKPRRRVPPSRAFEPTAPLAKPRIV
                     ERPAPAPAPTQKRVIALRPMSHGPAPDVIAIGSSTGGPQALFRILATFKGAVKQPIFI
                     TQHMPATFTTILAEHVANASGYPSAEAIDGEPVRAGHIYIAPGDFHMTIELGQGQNVV
                     RLNKGPQENFCRPSVDPMLRSLSARYGDRLFCAILTGMGHDGLAGSEVVVAAGGTVIA
                     QDQSTSVVWGMPGAVATAGLCSAVLPLDEIGPFIGKAAGRGWS"
     gene            4199..5044
                     /locus_tag="VGP50_05680"
     CDS             4199..5044
                     /locus_tag="VGP50_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEV8026897.1"
                     /translation="MTPGDFSFVSRLLKERSGLVLMQDKAYLLESRVLPIMRRRNMRS
                     LDEVVAALRRGDDALTREVVEAMTTNESSFFRDIKPFDQFRGFVLPHLMKARAQHRAL
                     RIWSAACSSGQEPYSLAMILTEEKAKLASWQTEIVATDLSTEILEKAQAGLYSQFEVQ
                     RGLPIQLLVKHFRQQGENWVIDPSIRAMVRYRVVNLLEEVTHLGQFDVIFCRNVLIYF
                     DMATKTKVLERISRVLKPDGYLFLGGAETIIGITDKFQSVAEQRGVYSPMMSPSAMAQ
                     RLAAR"
     gene            complement(5251..5970)
                     /locus_tag="VGP50_05685"
     CDS             complement(5251..5970)
                     /locus_tag="VGP50_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV8026898.1"
                     /translation="MRVLLVEDDSATAASIEMMLRAEGFICDTTDLGEDGLEIGKLYD
                     YDIIILDLMLPDIDGYEVLRRLRAARVRTPILILSGLAELDHKIKGLGFGADDFLTKP
                     FDRRELIARIQAIVRRAKGHSESVIRTGKLTVNLDSRTVEVDGQPLHLTGKEYGILEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFVCKLRKKLSTATKGDNYIETVWGRG
                     YVLRDPPAESAANAQRKLAKA"
     gene            6195..7574
                     /gene="fliI"
                     /locus_tag="VGP50_05690"
     CDS             6195..7574
                     /gene="fliI"
                     /locus_tag="VGP50_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HEV8026899.1"
                     /translation="MSVPLSSAADSIAPRQQQPVHRSDLVEDIALIPEYRRFGRVTGV
                     MGLLLEVGGLPRRLAVGDRCAVVAEKGPRLICEVVGFRAGRVLLMPFGAIYGVGLGCR
                     VEVEDRPPVIYPHDGWLGRVVNAMGEPIDGKGPLPLGPIAYPLRTAPPPAHERRRVRG
                     KIDLGVRALNTFLTCCRGQRMGIFSGSGVGKSVLLSMIARYTAADVTVIGLVGERGRE
                     VQEFIEDDLGEEGLARSVLVVATSDESALMRRQAAYLTMAIAEYLRDRDRDVLCLIDS
                     VTRFAMAQREIGLSAGEPPTTKGYTPTVFAELPKLLERAGPGRGAGSITGLFSVLVEG
                     DDHNEPVADAVRGILDGHIVLDRVIAERGRYPAVNVLRSLSRTMPGCNTAAENTTVGA
                     ARSLIAAYEDMAELIRLGAYKRGNDAKVDEAIRRYPLIEAFLAQRKDERASLASGYAQ
                     LAALLQAPV"
     gene            7571..7996
                     /locus_tag="VGP50_05695"
     CDS             7571..7996
                     /locus_tag="VGP50_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FliJ family protein"
                     /protein_id="HEV8026900.1"
                     /translation="MSALDQLIRLHRWRLDELRRHLSDVEALATKLEEQRRRLDSENT
                     REQSVAGASPEAAFAYPAYARQLIERRRKLAQSQSEALEQIAKARAALGEAFQEVKRY
                     ETAAASRLRQQQRREQARQQQALDEIGIDRFRRRGRESR"
     gene            complement(8114..9475)
                     /locus_tag="VGP50_05700"
     CDS             complement(8114..9475)
                     /locus_tag="VGP50_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026901.1"
                     /translation="MVDAIASVSSILAGSQPLSAVAASVPTSIELAQLPPQAIMPGVT
                     FTATVLGRGQNGALLLQSDQGTLALKTTLALAPGSSVTLRLAPGATSFVTLLRVDAPA
                     TAASGAGTSAPTPGSAGTTAAETTQSPPSPPAPARVEIGTVLKAVVLAPAQDGASEPL
                     PPGTGLVLRLTIPAAGGAGSAASADPILSGTIVTVTSSETVVATSIGTLALDRPLTLA
                     PGSTIEFQPLATVPPATASEAPPAAGHGWPALDQALAALDGFAPDIAARLRSDLTPTS
                     GEKLAGTLLFLMGTLSSGGWPDAKTTNALDRAGRSDLRLRLAGDLADLRQVADPPSGD
                     WRVFVLPLMDHNAVHPLRLYLRRRGGGNTRPEDGTRFVLDVEMSKLGALQLDGLVQQK
                     RLDVVLRSHQPIAPELRQEIVDVFRNATSAAGLAGDIVFTTASRFAVAPLDALHPHVA
                     IDA"
     gene            complement(9468..10283)
                     /locus_tag="VGP50_05705"
     CDS             complement(9468..10283)
                     /locus_tag="VGP50_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HEV8026902.1"
                     /translation="MKPTSVVTAFPQPGAAPLRRNIIAVASGKGGVGKTWFAITLAHA
                     LAKAGRKTLLFDGDLGLANVDVQLGFTPERDLGSVIAGDATLAQVASAYEGGFDIIAG
                     RSGSGSLAMLPPSRLLALRAELQLFAQNYDWVVLDLGAGIERTVRLLTGQSRICIVLA
                     TDEPTAITDAYAYIKVTALERLAEGIQIAVNMVPSQREGERVYATLLRACREFLKIEP
                     PLAGIIRRDQHVRDSIRRQTPLLTRHPTSEAAEDVAAIARRLDQELQRVASRG"
     gene            complement(10280..>11216)
                     /locus_tag="VGP50_05710"
     CDS             complement(10280..>11216)
                     /locus_tag="VGP50_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEV8026903.1"
                     /translation="PTMTEAMSLVRARLGEDAIIVSTEEDEDGNARVTAAVERLERPL
                     PTAGPDVVDILNEALSSHGLSPVLIEKILCTALTFHTDDPLLALAGALTALLRFTPVE
                     SAPRRPLLLIGPPGAGKTLTLAKLAARAVLAGTTPRLVTADTVRAGAAEQLEAFARVL
                     KLAVHRATTAARLGPLVAASKTSDLLLIDTAGINPYNAADRRELAAIIAASGAEPVLV
                     LPAGGDAVDTLETARIFRDLGAERLIVTRLDMTQRLGSVMGAAEALRLAFSEASATAD
                     VANGLTPCNAVVLARLLLPQPAEALRRANTRKASP"
BASE COUNT         1862 a         3716 c         3866 g         1772 t
ORIGIN      
        1 gcgcggccga tctgatgatg ggtgaaagcc gggaatcggc ggtggccgca cagagcatcc
       61 gcgtcaacgt ggacgtgctc gagaatttga tgacgatggt gagcgagctg gtgctgacgc
      121 gcaaccagct catgcagatc ctgcggacgc agaaggacac cgatttcgcc agcccgcttc
      181 agcgcctctc gcacatcacg accgagctgc aggaaggcgt gatgcgcacg cgcatgcagc
      241 cgatcggcaa tgcctgggcg aagctgccgc gcgtgctgcg cgatctctcc caggagctgg
      301 ggaagaggat cgatctccag atgttcggcg ccgataccga gctcgaccgc caggtcctgg
      361 agatggtcaa ggatccgctg acccacatgg tgcgcaactc cgccgaccac gggatcgaat
      421 cgcccgagga acggctgcgc gagggcaaac cggagaccgg caccatcacg ctcaacgcct
      481 atcacgaggg tggccacatc atcatcgaga tcagcgacga cggacgcggc atcgatctcg
      541 aacgcatcaa gcagaaggcg gtaagcgtcg gcctcgcgag cgagagcgag gtcgcggcga
      601 tgaccgagca gcagatccat cagttcatct tccgccccgg tttttccacc gcgaccagcg
      661 tgaccaacgt ctcgggccgc ggcgtcggca tggacgtcgt gcgcaccaac atggagaaga
      721 tcggcggcac ggtggagatg aagagccgcg agaacaaggg ctcgaccttc gtcatcaaga
      781 ttccgctgac cctcgccatc gtctcggcgc tcatcgtcga gtgcggcggc gagcgcttcg
      841 ccataccgca gatcagcgtc gtcgagctgg tgcgggccgc cgcgcatgcc gatgtgagta
      901 tcgagtggct caacaacgcg ccggtgctgc gcttgcgcga ccgcctgctg ccgctggtct
      961 cgctctacga gctcttgaag ctcggacggc cggcggaccg gccgaaagag accttcatcg
     1021 tcgtgacgca ggtcggcaca cacagcttcg gcatcatcgt cgaccgcgtc tacgacaccg
     1081 aagagatcgt cgtgaagccg gtggcgccga tcctccgcaa catcccgatg ttctcgggaa
     1141 acacgatcct cggcgacggc agcgtcatca tgatcctcga tccgaacggg atcgcggcga
     1201 gcgccggcga gattgccggt aacgcgagcg gtgtccgggc ggagccggtg gcggcgcgcg
     1261 cggtggtgcg cgccaacctg acaagcctcc tgctgttccg cgtcggcggc ggcgcgccca
     1321 aggcagtgca gctctcgctc atcgcccggc tcgaggagat cgatctcgcg cagatcgagt
     1381 actcgaacga gcggccggtc gtgcagtatc gcggcaagct catgccgatc gtcaccatcg
     1441 atcagaccgc gccgtccggc gaaggccgcc ggccgatcct ggtcttcgcc gacaaggacc
     1501 acatcatggg cctcgtggtc gacgagatcg tcgacatcgt cgaggaggag ctcaagatcg
     1561 agctcacgat cgaccggccc ggcctcgtcg gcagcgcggt gatcgccggc aaggcgaccg
     1621 acgtcatcga cgcgggctac tacgtcaacc agacctacag cgattggttc ggcaagccga
     1681 cgctggccat ggagagccag agccaggccg agtcgtcgga aggcggcaaa cgcctgctgc
     1741 tcgtcgacga cagcccgttc ttccgcaaca tgctgacgcc gctgctcgcg gcggcgggct
     1801 atgccgtgac gacggccgat tccggcgacc acgcgctgca aatctgcgcg tcgggcgagg
     1861 atttcgacat catcgtcagc gacatcgaga tgcccggaat gaacggcttc gagttcgcct
     1921 ccgccgtcag gagcgacagc cgctggcagt cgacgccgct ggtggcgctc tcggcccatg
     1981 cctcccagaa ggacatcgat cgcgggcgtg ccgcgggctt cagcgactac gtgccgaagt
     2041 tcgaccgcga agcgctcctc gctacgctgt ccgaagcgat caacgagcga ggtgcggcat
     2101 gagcatgatc accatccgca gcgcgccgct cgacgcggcc gccgccagcg acgaagtgca
     2161 tgagctggtc tccgtcacgg tcgcggacca gattttcggc attcccgtgc tgcaggtcca
     2221 ggacgtgctc gggccgcagc gcatcacgcg gatcccgctg gcgccgcccg aggtcgcggg
     2281 ctcgctcaat ctgcgcggcc gcatcgtcac cgccatcgac ctgcgcacgc ggctccggct
     2341 gccgccgctg cctgaggcgc gcaagagcat gagcgtcgtc gtcgatcacg agggcgagct
     2401 ctacagcatt ctcgtcgacg gtgtcggcga ggtgctgaag ctcaacggca acagcgccga
     2461 gcgcaatccg ccgacgctgg acgcgacgtg gcgcgacgtc tcggcgggag tctatcgcct
     2521 ggagaagacc attctcatcg tgctcaacgt cgaacgtgtc ctcgatttct cggcccgggc
     2581 cgacgccatc taaggggatc atcagtggtg cagtccgcac aagcccggag caaagggcag
     2641 aagaagttct gcctgatcgt cgacgactcg cgcgtcgtgc gcaaagtcgc gcggcgcatc
     2701 ctcgaggatc tgagcttcgc catcgaggag gcgaccgacg gcaagacggc gatggaagcg
     2761 tgtctgcgca agatgccgga cgtcgtgctg ctcgactgga acatgccggt catgagcggc
     2821 atcgacttcc tgcgccagct gcggcgcacc aagggcggcg acgtgcccgt ggtggtgttc
     2881 tgcaccacgg agaacgacgt ccatcacatc aaggaagcgc tgggcgccgg ggcgaacgag
     2941 tacatcatga agccgttcga cagcgatatt ctcgaggcca agttcgcgca ggtgggactg
     3001 ttgtgagcac tgccgtggcg gcgccgcttg cgggatcgca ggcgccgggg ggcgaaccct
     3061 accggctcat ggtcgtcgat gacagcgcca tcgtccgcgg cctgctcatc cgctcgctcg
     3121 aggcggatcc ggaagtcacc gtggtggcat cggccagcaa cggcgagatc gcgctggcgg
     3181 ctctcggccg ccacgacgtc gaagtcgtca ttctcgacat cgagatgccg gtcatggacg
     3241 ggcttaccgc gcttccgaag atgctggccg cgaagcccgg cctcaaagtg ctgatcgcct
     3301 cgtcgctgac gcggcgcggc gccgagatca gcctgcgcgc gctcgccgcg ggcgcggccg
     3361 actacctgac gaagcccggc acgtcggcgc tgaccagcgc cgacgagttc aagcacgaat
     3421 tgctcgtcaa agtgaaggcg ctcgcccagg cgggccgctc ggcgctcggc atcaccaagc
     3481 cgcgccggcg ggtaccgccg tcccgcgcct tcgagccgac ggcgccgctg gcgaagcccc
     3541 ggatcgtcga gcgtccggcg ccggcccccg ccccgacgca aaagcgcgtg atcgcgctcc
     3601 ggccgatgag ccacgggcct gcgcccgacg tcatcgccat cggctcctcg acgggtgggc
     3661 cccaagctct cttccgcatc ctggcgacgt tcaagggcgc ggtgaagcaa cccatcttca
     3721 tcacacagca catgccggcg accttcacca ccatccttgc cgagcacgtc gccaatgcca
     3781 gcggctatcc gagcgccgaa gccatcgacg gcgagccggt gcgggccggg cacatctaca
     3841 tcgcgcccgg cgatttccac atgaccatcg agctggggca ggggcagaac gtggtgcggc
     3901 tcaacaaggg accgcaggag aatttctgcc ggccgtcggt cgatcccatg ctccgcagcc
     3961 tctcggcgcg ctacggcgat cgcttgttct gcgccatcct cacgggcatg ggccatgacg
     4021 gcctcgccgg cagcgaggtc gtggtcgccg ccggcggcac ggtgatcgcg caggaccagt
     4081 cgaccagcgt ggtttggggc atgcccggag ccgtcgcgac ggcgggtctc tgcagcgccg
     4141 tcctgccgct cgacgagatc ggacccttca tcggcaaggc tgccgggcgg ggctggtcat
     4201 gacgccgggc gatttcagct tcgtctcgcg cctcctcaag gagcgctcgg gcctggtgct
     4261 gatgcaggac aaggcctatc tgctcgagag ccgggtgctg ccgatcatgc ggcggcgcaa
     4321 catgcgctcg ctcgacgaag tcgtggcggc cttgcgccgc ggcgacgacg cgctgacgcg
     4381 cgaagtggtc gaggccatga ccaccaacga atcctcgttc ttccgcgaca tcaagccgtt
     4441 cgaccagttc cgcggcttcg tcctgccgca cctcatgaaa gcgcgtgccc agcaccgcgc
     4501 gctgcgcatc tggagcgcgg cctgctcgag cggtcaggag ccttattcgc tcgccatgat
     4561 cctgaccgag gagaaggcga agctcgcgag ctggcagacc gagatcgtcg ccaccgatct
     4621 gtccaccgag atcctcgaaa aggcccaggc gggcctctat tcgcaattcg aggtgcaacg
     4681 tggcctgccg attcagctgc tggtcaagca tttccgccag cagggcgaga actgggtgat
     4741 cgacccttcg atccgcgcca tggtgcgcta tcgcgtcgtc aacctgctcg aggaagtgac
     4801 tcatctcgga caattcgacg tcatcttctg caggaatgtg ttgatctatt tcgacatggc
     4861 gaccaagacg aaggtgctgg agcggatttc gcgcgtgctc aagcccgacg gctatctctt
     4921 cctcggcggc gcagagacga tcatcggcat caccgacaag ttccagtcgg tcgccgagca
     4981 gcgcggcgtc tactcgccga tgatgtcgcc gagcgcgatg gcgcagcggc tggcggcgcg
     5041 ctaacgcgaa cgcgcgaagc gcgttcgtcg ttggcggcga cagcgattgc gcagcaatcg
     5101 cgagagccgc acagatcgga ttagcggaaa tgaacccctc accctaccgc tcctacaagg
     5161 ggagagggga atggcgcgct tgcgcccaaa gcaaaacaaa gcggccgcgc acgtggcgcg
     5221 gccgctccga ctccccgcgg aaatctgtgt ttacgctttc gcgagcttgc gctgggcgtt
     5281 ggccgcggat tcggccggcg ggtcgcgcag cacatagccg cggccccaga cggtctcgat
     5341 gtagttgtcg cccttggtcg ccgtcgagag tttcttgcgc agcttgcaga cgaagacgtc
     5401 gatgatcttg agctcgggct cgtccattcc gccgtagaga tggttcagga acatctcctt
     5461 ggtgagcgtc gtccccttgc gcaggctcaa cagctcgagg atgccatact ccttgccggt
     5521 gaggtggagc ggctggccgt cgacctcgac ggtgcggctg tcgagattga cggtgagctt
     5581 gccggtgcgg atcaccgatt ccgaatgacc cttggcgcga cgcacgatcg cctggatgcg
     5641 ggcgatcagc tcgcggcggt cgaaaggctt ggtaaggaag tcgtcggcgc cgaagcccag
     5701 acccttgatc ttgtgatcga gctcggcaag gccggagagg atcaggatcg gcgtgcggac
     5761 gcgggcggcg cgcagccggc gcagcacttc gtagccgtcg atgtccggca gcatgaggtc
     5821 gagtatgatg atgtcgtagt cataaagctt gccgatctcg aggccgtctt cgccgagatc
     5881 ggtggtatcg caaatgaagc cttcggcccg cagcatcatc tcgatgctag cggcggtcgc
     5941 tgaatcatct tccaccagaa gtacgcgcat ctgtcccgcc tccgattgac ccgacgtctc
     6001 tcagccgcct gtccgccaac accataaacg cttttaatca ttcttaccaa ccgaggttaa
     6061 agccaggtta accaagcaac gaaaaagtac cctttctcgc aattattacg cgtgacggtt
     6121 gaccgctatg cctgggtatt ccgggccggc tcggcaaatc gcacttaacc ggggattaaa
     6181 cacgggaggc gaagatgagc gtgccgctca gctccgcggc cgacagtatt gcccccaggc
     6241 agcagcagcc cgtgcatcgc tccgatctcg tcgaggacat cgcgctcatc ccggaatacc
     6301 gccgctttgg ccgggtcaca ggggtcatgg gactgctgct cgaggtcggg gggctgccgc
     6361 gccggctggc cgtcggcgac cgctgcgcgg tcgtcgccga gaagggcccg cgcctcatct
     6421 gcgaggtcgt gggctttcgc gccggacgcg tattgctgat gccgttcggc gccatctacg
     6481 gtgtcggact cggctgtcgc gtcgaggtcg aggaccggcc gcctgtaatc tatccgcacg
     6541 atggctggct cggccgtgtc gtcaacgcga tgggcgaacc gatcgacggc aaggggccgt
     6601 tgccgctcgg ccccatcgcc tacccgttgc gcaccgcgcc gccgccggcc cacgaacgcc
     6661 gtcgcgtgcg gggcaagatc gatcttggcg tgcgcgcctt gaacaccttt cttacctgct
     6721 gtcgcggtca gcgcatgggc attttctccg gcagcggcgt cggcaaatcg gtgctgctgt
     6781 cgatgatcgc ccgctacacc gccgcggacg tgaccgtgat cggcctcgtc ggcgaacgcg
     6841 ggcgcgaggt tcaggaattc atcgaagacg atctcggtga agaagggttg gcccgcagcg
     6901 tcctcgtggt cgccacctcc gacgaatcag cgctcatgcg gcgccaggcc gcctatctca
     6961 ccatggcgat cgccgaatac ctgcgcgacc gcgaccgcga cgtgctctgc ctcatcgaca
     7021 gcgtcacgcg cttcgccatg gcccagcgcg aaatcgggct ctcggccggc gagccgccga
     7081 ccacgaaggg ctacacgccg accgtctttg ccgagctgcc gaagctcctc gaacgggcgg
     7141 gcccgggcag gggcgccggc agcatcaccg ggctcttcag cgtgctggtc gagggtgacg
     7201 accataacga gccggttgcc gatgcggtgc gcggcatcct cgacggccat atcgtgctcg
     7261 atcgcgtcat tgccgagcgc ggccgctatc ccgcggtcaa cgtgctgcgt tcgctttccc
     7321 gcaccatgcc cggctgcaac accgcggcgg agaatacgac cgtcggcgcc gcgcggtcgc
     7381 tcatcgccgc ctatgaagac atggccgagc tgatccggct cggcgcctat aagcgcggca
     7441 acgatgccaa ggtcgacgaa gcaatccgtc gctatccgtt gatcgaggcg ttccttgcgc
     7501 agcgcaagga cgagcgggca agtctcgcca gcggctatgc gcagctcgcc gcgctgttgc
     7561 aggcgccggt atgagcgcgc tcgatcagct cattcgcctc caccggtggc ggctcgacga
     7621 gctgcgccgc catctgagcg atgtcgaggc gctcgcgacg aagctcgaag agcagcgccg
     7681 ccgtctcgac agcgaaaata cgcgcgagca gtctgtggcc ggcgcctctc ccgaggcggc
     7741 cttcgcctat cccgcctatg cgcgccagct catcgagcgg cggcgcaagc ttgcccagtc
     7801 gcagagcgag gcgttggaac agatcgccaa ggcacgggcg gcgctcggcg aggcgttcca
     7861 ggaggtgaag cgctacgaga cggcggcggc cagccggctg cgccagcagc agcggcgcga
     7921 gcaggcgcgg cagcagcagg cgctcgacga aatcggcatc gaccgcttcc gccggcgtgg
     7981 ccgggagtcg cgatagggaa acggcgccgg tggcgcctca agcgcggccc gcctaattct
     8041 gatctgagcg gctttcgcga ttgctgcgca atcgctggcg ccgcggcgga cgaacgcgca
     8101 aacgcgcgtt cgcctaggcg tcgatcgcga cgtgggggtg aagcgcatcg agtggcgcca
     8161 cggcgaagcg ggaagcggtc gtgaagacga tgtcgccggc caggccggcc gccgatgtcg
     8221 cgttgcggaa cacgtccacg atctcctgac gaagctcggg ggcgatcggc tgatggctgc
     8281 gcagcacgac gtcgaggcgc ttctgctgca ccaggccgtc gagctggagc gcgcccagct
     8341 tgctcatctc gacatcgagg acgaagcgcg ttccatcttc gggtcgcgtg ttgccgccgc
     8401 cgcggcggcg caggtagagc cgcagcggat gcacggcatt gtgatccatg agcggcagca
     8461 cgaagacgcg ccagtcgccg ctcggtggat cggcgacctg ccgcagatcc gccaggtcgc
     8521 cggcgaggcg caggcgaaga tcgctccgtc cggcgcggtc gagcgcattc gtcgtcttcg
     8581 cgtcgggcca gccgccgctg ctcagcgttc ccatgaggaa gagcagcgtg ccggcgagct
     8641 tctcgcccga ggtcggcgtg agatcgctgc gcagccgcgc tgcgatgtcg ggcgcaaatc
     8701 catcgagagc cgcaagcgcc tggtccagcg ccggccagcc atggccggcc gccggggggg
     8761 cttcgctcgc tgtcgcaggc gggaccgtgg cgagcggctg gaactcgatg gtgctgccgg
     8821 gcgcgagcgt cagaggccga tcgagggcga gcgtgccgat ggacgtggcc acgaccgtct
     8881 cgctgctggt gacggtgacg atcgtgccgg acaggatagg gtctgccgac gcggcgctgc
     8941 cggcgccgcc cgctgcgggg atcgtcaggc gcagaacgag accggtcccg ggcggcaacg
     9001 gctcgctggc gccgtcttgg gccggcgcca gcacgacggc tttgagcacc gtgccgatct
     9061 cgacgcgggc cggcgcaggg ggcgatggcg gagactgcgt cgtctctgcc gccgtggtgc
     9121 cggcgctgcc gggcgtcggc gcagatgtcc cagcgcccga agccgcggtg gccggcgcat
     9181 cgacacggag cagcgtcacg aacgacgtcg cgccgggggc gagccgcagg gtgacgctgc
     9241 ttccgggggc gagcgccagc gtcgtcttga gcgcgagcgt cccttgatcg gactgcagca
     9301 gcagcgcccc gttctggccg cggccgagca cggtcgcggt gaaggtgacg ccgggcatga
     9361 tggcctgtgg cggcagctgc gcaagctcga tcgacgtcgg cacgctggcg gcgaccgcgg
     9421 acaagggctg ggatccggcg aggatcgaag agacgctggc gatggcgtca accacggctt
     9481 gccacgcgct gcagctcctg atcgaggcgg cgggcgatcg ccgcgacgtc ttccgccgcc
     9541 tcgctggtcg gatgccgggt cagcagcggc gtctggcggc ggatgctgtc tctcacatgc
     9601 tggtcgcggc ggatgatgcc ggcgagcggc ggctcgatct tcaggaactc acggcaggcg
     9661 cgcagcaacg tggcatagac gcgctcgccc tcgcgctggc tcgggaccat gttgacggcg
     9721 atctgtatgc cctcggccag ccgttccaac gcggtgacct tgatataggc gtaggcgtcg
     9781 gtgatggccg tgggctcgtc ggtggcgagg acgatgcaga tgcgcgattg tccggtgagc
     9841 agccgcaccg tgcgctcgat gccggcgccg agatcgagca ccacccagtc gtagttctga
     9901 gcgaaaagct gcagctccgc ccgcagcgcc aggagccggc tcgggggaag catcgcgagg
     9961 ctgccggagc cggagcgtcc cgcaatgatg tcgaatccgc cctcataggc gctggccacc
    10021 tgtgccagcg tggcgtcccc ggcgatgacg ctgccgagat cgcgctcggg cgtgaagccc
    10081 agctgcacgt cgacattggc gaggccgagg tcgccgtcga agagcagcgt cttgcgtcct
    10141 gccttggcca gcgcatgcgc gagggtgatc gcgaaccagg tcttgcccac gccgcctttg
    10201 cccgaagcga cggcgatgat gttgcgacga agcggcgcgg cgccgggctg cggaaacgcc
    10261 gtcaccacgg atgttggctt catggcgatg ctttcctggt attggcccgg cgcagcgcct
    10321 ctgccggctg cggcaggagc aggcgcgcga gcaccacggc gttgcaggga gttagtccgt
    10381 tcgcgacgtc ggcggtggcg ctcgcctcgc tgaatgcgag acgcaacgcc tcggccgcac
    10441 ccatgacgct gcccaggcgt tgcgtcatgt cgaggcgggt gacaatgagg cgctcggcac
    10501 caaggtcgcg gaagatgcgc gccgtctcga gcgtgtcgac ggcatcgccg ccggccggca
    10561 gcacgagcac aggctcggcg ccgctggcgg cgatgattgc ggcgagctcg cgccgatcgg
    10621 ccgcgttata gggattgatg ccggccgtat cgatgagcag caggtcgctg gtcttgctgg
    10681 cggcgacgag cgggccgagg cgcgccgcgg tggtggcgcg atgcaccgcc agcttcagca
    10741 cgcgagcgaa ggcttcgagc tgctcggcag cgccggcgcg cacggtgtcc gcggtaacga
    10801 ggcgcggcgt cgtgccggcc agcacggccc gagccgcaag cttggcgagg gtcaaagtct
    10861 tgccggcgcc gggcggcccg atcagcagca gcgggcggcg cggcgcggac tcgacaggcg
    10921 tgaatctgag cagcgccgtc aacgcccctg ccagcgccag cagcgggtcg tcggtatgga
    10981 acgtcaaggc cgtgcagaga atcttctcga tgagaaccgg gctgaggcca tggctgctca
    11041 gcgcctcgtt caggatgtcc acgacatccg ggccagcggt ggggagtggc cgctccaggc
    11101 gctcgactgc cgcggtgaca cgggcgttgc cgtcttcgtc ctcctcggtg gagacgatga
    11161 tcgcgtcctc gccgaggcgc gcgcgcacca ggctcatggc ctcggtcatc gttggc
//
LOCUS       DASYNS010000183         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_351971, whole genome shotgun sequence.
ACCESSION   DASYNS010000183 DASYNS010000000
VERSION     DASYNS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351971"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VGP50_05715"
     CDS             complement(<1..516)
                     /locus_tag="VGP50_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEV8026904.1"
                     /translation="MASNKKILVLPGDGIGPEVMRQVYRVVEWFARKRIASFELSEDL
                     VGGAAYDAHGTPLADKTLQAALAADAVLFGAVGGPKWDTLPFPLKPERGLLQLRKEME
                     LFANLRPALVFEPLIGASTLKPEVIRDLDLMIVRELTGGVYFGEPRGIEVLSDGTRRG
                     VNTQVYTTPEIL"
     gene            complement(714..1325)
                     /gene="leuD"
                     /locus_tag="VGP50_05720"
     CDS             complement(714..1325)
                     /gene="leuD"
                     /locus_tag="VGP50_05720"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV8026905.1"
                     /translation="MDKFTTLDGIAAPLPMINVDTDKIIPKQHLKTIERTGLGKALFD
                     EMRYRDDGSENPDFILNKPAYRQAKILVAGENFGCGSSREHAPWALLDFGIRCVIAQS
                     FADIFYNNCFKNGILPIRLPREIVDALMDDARKGANARLTIDLAAQTITRPDGAAVTF
                     EIDPFRKHCLLNGLDDIGLTLDKAPAIAGFEEKNRLSQPWLWS"
     gene            complement(1336..>2041)
                     /locus_tag="VGP50_05725"
     CDS             complement(1336..>2041)
                     /locus_tag="VGP50_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEV8026906.1"
                     /translation="VAPDETTFAYLQGRPHAPKGAGWEQALKFWKTLPSDPGARYDTE
                     IAIDAAKIVPQVTWGTSPQDVLPITGVVPNPAEISDESRRQAMERSLEYMGLTPGTPL
                     MEVPVARVFIGSCTNGRIEDLREAAAVAEGRQVAAGVAAMVVPGSGLVKHQAEEEGLD
                     RIFIAAGFEWREPGCSMCLAMNADKLLPGERCASTSNRNFEGRQGRGGRTHLMSPAMA
                     AAAAVTGRLADVRALG"
BASE COUNT          309 a          676 c          711 g          345 t
ORIGIN      
        1 gaggatctcg ggcgtggtgt agacttgcgt gttgacgccg cggcgcgtgc cgtccgacag
       61 cacctcgatg ccgcgcggct cgccgaaata gacgccgccc gtcagctcgc gcacgatcat
      121 gagatcgaga tcgcggatca cctcgggctt caaggtcgag gcgccgatca gcggctcgaa
      181 gaccagcgcc ggccgcagat tggcgaagag ctccatctcc ttgcgcagct gcaggagtcc
      241 gcgctcgggc ttcaagggga agggcagcgt gtcccatttc gggccgccca ccgcgccgaa
      301 caggacggcg tccgccgcga gcgccgcctg cagcgtcttg tccgcgaggg gcgtgccatg
      361 ggcgtcataa gccgcgccgc cgaccagatc ttcgctcagc tcgaaagacg cgatgcgctt
      421 cctcgcgaac cattcgacga cgcgatagac ctgccgcatc acctcggggc caatgccgtc
      481 gccggggagg acgaggatct tcttgttcga agccatgatg agtcgccgct agatctgccc
      541 ctctcccgca tcgcgggaga gggtggcgcg cgcgaagcgc gcgccgggtg aggggacgtt
      601 ggcggaggac actcgcttta gagatcccct caccctccca tcgcttcgcg atgggtccct
      661 ccctctcccg cgatgcggga gaggggcaat tttctgcggc gtggccgcgc ttctcacgac
      721 cagagccaag gttggctcag gcggttcttt tcctcgaagc cggcgatggc cggggccttg
      781 tcgagggtga ggccgatgtc gtcgaggccg ttcagcaggc aatgcttgcg gaaggggtcg
      841 atctcgaagg tgacggcggc gccgtcgggc cgcgtgatgg tctgcgccgc gaggtcgatg
      901 gtgagccgcg cattggcgcc cttgcgcgca tcgtccatca gcgcgtcgac gatttcccgc
      961 ggcagcctga tcggcaggat gccgttcttg aagcaattgt tgtagaagat gtcggcgaag
     1021 ctctgggcga tgacgcagcg gatgccgaaa tcgagcagcg cccagggcgc gtgctcgcgc
     1081 gagctgccgc agccgaaatt ctcgcccgcc accaggatct tcgcctgacg ataggcgggc
     1141 ttgttgagga tgaagtccgg attctccgag ccgtcgtcgc gatagcgcat ctcgtcgaag
     1201 agcgccttgc cgaggccggt gcgctcgatc gtcttcagat gctgcttcgg aatgatcttg
     1261 tcggtgtcga cattgatcat gggcagcggt gcggcgatcc catcgagggt cgtgaacttg
     1321 tccataaacg cctcctcaac cgagcgcgcg cacgtcggcg aggcggcccg tcacggcggc
     1381 cgccgcagcc atggccgggc tcatgagatg ggtgcggccg ccgcggccct ggcggccctc
     1441 gaaattgcgg ttcgaggtgg aggcgcagcg ctcgccgggg agcagcttgt cggcattcat
     1501 ggcgaggcac atggagcagc cgggctcgcg ccattcgaag ccggccgcga tgaagatgcg
     1561 gtcgagcccc tcctcctccg cctggtgctt caccaggccc gagcccggca cgaccatggc
     1621 ggcgacgccg gccgcgacct ggcgcccctc ggccacggcg gcggcctcgc gcagatcctc
     1681 gatccggcca ttggtgcagg agccgatgaa gacgcgggcg accggcacct ccatgagcgg
     1741 cgtcccgggc gtcaggccca tatattcgag cgatcgctcc atcgcctggc gccggctctc
     1801 gtcagagatc tccgccgggt tcgggacgac gccggtgatc ggcagcacgt cctgcgggct
     1861 cgtaccccag gtgacttgcg gcacgatctt cgccgcgtcg atcgcgatct ccgtgtcgta
     1921 gcgcgcgccg gggtcggacg gcagcgtctt ccagaatttg agcgcctgct cccagccggc
     1981 gcccttgggc gcatgcggcc gcccctgcag ataggcgaag gtcgtctcgt cgggcgcgac
     2041 g
//
LOCUS       DASYNS010000184         7024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1758197, whole genome shotgun sequence.
ACCESSION   DASYNS010000184 DASYNS010000000
VERSION     DASYNS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758197"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /gene="leuB"
                     /locus_tag="VGP50_05730"
     CDS             <1..735
                     /gene="leuB"
                     /locus_tag="VGP50_05730"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEV8026907.1"
                     /translation="VIRDLDLMIVRELTGGVYFGEPRGIEVLSDGTRRGVNTQVYTTP
                     EILRVGRVAFDLARKRRGKVCSVEKANVMESGQLWREEMQKLHDAEYKDVELSHMYAD
                     NCAMQLVRNPKQFDVIVTDNLFGDLLSDCAAMLTGSLGMLPSASLGAADSSGRRKALY
                     EPVHGSAPDIAGKDVANPLACLLSFAMMLRYSFDMKEEAGMVEGAVQSALKSGVRTTD
                     ILQPGTARVSTTVMGDTVLHALDKLA"
     gene            826..2430
                     /locus_tag="VGP50_05735"
     CDS             826..2430
                     /locus_tag="VGP50_05735"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEV8026908.1"
                     /translation="MRFVALGASLAGMVVFAALIAWSGAFVVPALVCAVLSAVGVWDW
                     LQPRHSLRRNYPILANIRFGLEMIRPEIRQYFLESDLDGAPFNRSKRAVVYQRAKGAL
                     DKRPFGTLYDVYANNFEWINHSIGARPIRDSDFRVTVGGDACRHPYALSLFNVSAMSF
                     GALSANAIRALNRGAKLGGFAHDTGEGGFSRYHREFGGDIIWEIGSGYFGCRNEDGSF
                     SPRRFAEEAAAAQIRMIEIKLSQGAKPGHGGVLPGAKVTAEIAQARGVPVGVDCVSPA
                     GHSAFATPLEMVRFIGELRELSGGKPVGFKLCIGHPWEFMAICKAMLETGIFADFVVI
                     DGTEGGTGAAPLEFVDHVGTPLREGLVFAHNCLVGAGIRERLRIGASGRIVTAFDMAR
                     VLALGADWCNSARGFMFALGCIQSQSCHTDRCPTGVATQDQLRQRALVVPDKAERVRH
                     FHDNTLKALAELLGAAGLSHPSELRPAHILKRLSPSTVATFAEVYPGLAPGDLVNGTA
                     TERYQRCWALADARSFAPREDLRPAA"
     gene            2595..4664
                     /locus_tag="VGP50_05740"
     CDS             2595..4664
                     /locus_tag="VGP50_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV8026909.1"
                     /translation="MEAERRPVTILFADLVGFTAFTERSGEEAAYVLMEAIHRLMERA
                     VEAHGGTIKEATGDGVVALFGVPFALEDAPLRACRAALSIQQCLAEEVDAVAAGVRPQ
                     LRISINTGAVIVGPVEEGAGLAVFGDTVNTAARLQACAPPGAILLSEATRKLVLGLVD
                     CEFVGLCRLKGKAEPQPAYRLDAIRQRVSRFQAVLARGLSPYVGRAGERKRLEGLLCG
                     GRRRLRVIDIVGEPGIGKSRLLHTVLSGNAESEPILSGSCSPDGSAVPFLPLIDVVRG
                     CLGIAEGEDQEGIARKLAGGLAALELFSVANVDLLLNLLGLAASPEGAAIGLRTGDLL
                     RRLLQARCRRSPLVVLIEDLQWIDSASEEVLTQIVATTADASRLVLIHTRRPEYLPPW
                     IDHASTTTVPLSSLSAAETSCIAQARLGGGELPGEFARLIVERAGGNPLFAEEIAGSL
                     LERRIVRRGAAGLQYEPSIGAAVLPLSVHSLLAARIDGLDPRDRALLRSAAAIGYRFD
                     ADILGAVIGDDGGLDRRLAALQQLGLLQAGDGPRAFVFKHALVRDALYDSLLAAPRAA
                     LHLRIGSAIERRSSDRRIEAAEIVAGRWRTAHGAGKASSVRNRAAATRAGTHCLKEAE
                     RHFALALQVAATFPFGPTTRGSTSLAAPVRAVAVPEEKTEEAQRNGRASGSTWSMAGL
                     GWLDKLA"
     gene            complement(4672..5469)
                     /locus_tag="VGP50_05745"
     CDS             complement(4672..5469)
                     /locus_tag="VGP50_05745"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEV8026910.1"
                     /translation="MQPPAPEAYFRSLEDIGIRVPPAMPQPIDAEIEKLVAQIAGTTR
                     GAGIVIAAQEIARDPSASTKLRTVLALLKLLGFGRAQQTIDALQAEAPKLGCREGCAY
                     CCYQSVEVTIPEAILVAVHLADPGDPRRKALLARIDAQASKSKPERRHAGRPCPLLVD
                     NRCSVYEDRPLMCRSIFAADSADCRASLESIQRGGPELPVEQFLTAQYLMLSDQAGIR
                     GICKDMGLQHDLVELSEGVAAILRDPGIIERWISGERVFGPELTIES"
     gene            5575..6645
                     /locus_tag="VGP50_05750"
     CDS             5575..6645
                     /locus_tag="VGP50_05750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV8026911.1"
                     /translation="MDFGYFTLSDNHYAGNRRGANQLVADILDEVVYADALGYHSAWI
                     GEHHFNSLGVLSCPDLVLAHAAARTRHIRLAPAVTVLPLHHPIRVAEQWATLDLLSGG
                     RVDWAAGRGYDRREYEPFNVDFSDNQAIFEEGMELVRRLWDAGDTRISHEGRYYHFEN
                     VRITPQPVQKPIPAYVASFSKPSIELAGRLGCGLVVAPFAAAMTFGGLRQVAALYHES
                     CARHGKRPGRLMCSYFAHFADDAEQEAAARARQIRYYRECVIPALPGDPKTAPPSYRY
                     FIDMVDRLGKVTPADLTENSVLLGSAQQITDTLKKVEAAGFDEVILYFNVGLKPHAQV
                     KDEMARFMTEVAPAFKRAPARG"
     gene            complement(6731..>7024)
                     /locus_tag="VGP50_05755"
     CDS             complement(6731..>7024)
                     /locus_tag="VGP50_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEV8026912.1"
                     /translation="GLGGIFVELLKDVTFRLAPFDESEARRMIGELRGAAVLAGLRGR
                     PPADIAALAQALAALSRFAAAQGPTLRSLDINPLLVLPAGEGVLALDAVVVPA"
BASE COUNT         1095 a         2389 c         2356 g         1184 t
ORIGIN      
        1 gtgatccgcg atctcgatct catgatcgtg cgcgagctga cgggcggcgt ctatttcggc
       61 gagccgcgcg gcatcgaggt gctgtcggac ggcacgcgcc gcggcgtcaa cacgcaagtc
      121 tacaccacgc ccgagatcct ccgcgtcggc cgcgtcgcct tcgaccttgc gcggaagcgc
      181 cgcggcaagg tctgctcggt cgagaaggcc aatgtcatgg aatcgggcca gctgtggcgc
      241 gaggagatgc agaagctgca cgacgccgag tacaaggacg tcgaactctc gcacatgtat
      301 gccgacaact gcgccatgca gctggtgcgc aacccgaagc agttcgacgt catcgtcacc
      361 gacaatctct tcggtgatct cttgtcggat tgcgcggcga tgctcacggg ctccttgggc
      421 atgctgcctt cggcctcgct gggcgccgcg gattcgagcg gccggcgcaa ggcgctttac
      481 gagcccgtcc atggcagcgc gccagacatc gccggcaagg acgtggccaa cccgctcgcc
      541 tgcctcctct ccttcgcgat gatgctgcgc tactccttcg acatgaagga ggaggcgggc
      601 atggtcgagg gcgccgtgca gagcgcgctc aagagcggcg tgcgcaccac cgacatcctt
      661 cagcctggca ccgcccgcgt ctcgaccacg gtgatgggcg acaccgtcct ccacgccctc
      721 gacaagctcg cctgaggcga agcgcgcgga cttgcgaatg tcatggaccg cgcccgattc
      781 tatggcagag tcggacggcg ctaaacgaac gtgggggagg cgcaaatgcg gttcgtggcg
      841 ctgggcgcga gcctggccgg catggtggtc ttcgccgccc tcatcgcctg gtccggcgct
      901 ttcgtcgttc ctgccctcgt ctgcgcggtc ctgagcgcgg tcggcgtctg ggactggctc
      961 cagccgcggc acagcctgcg ccgcaactat ccgatcctcg ccaatatccg cttcggcctc
     1021 gagatgatcc ggcccgagat ccgccaatac tttctcgaga gcgacctcga tggcgcgccc
     1081 ttcaaccgct cgaaacgcgc cgtcgtctat cagcgcgcca agggagcgct cgacaagcgg
     1141 cccttcggta cgctctacga cgtctacgcc aacaatttcg agtggatcaa ccattcgatc
     1201 ggcgcgcgtc cgatccgtga ctccgatttt cgcgtgacgg tgggcggcga cgcctgccgc
     1261 cacccctatg cgctctcgct cttcaacgtc tcggcgatga gcttcggcgc gctctcggcc
     1321 aatgcgatcc gcgccttgaa ccggggcgcc aagctcggcg gcttcgccca tgacacgggc
     1381 gagggcggct tcagccgcta tcaccgcgag ttcggcggcg acatcatctg ggagatcggc
     1441 agcggctatt tcggttgccg caacgaggat ggcagcttct cgccgcgccg cttcgccgag
     1501 gaggcggccg cggcgcaaat aaggatgatc gagatcaagc tgtcgcaagg agcgaagccc
     1561 ggccatggcg gcgtcctgcc cggggccaag gtgacggccg agatcgcgca agcgcggggc
     1621 gtgcctgtgg gcgtcgactg cgtctcgccg gcgggccact ccgccttcgc gacgcccctc
     1681 gagatggtgc gcttcatcgg cgaactgcgc gagctttccg gcggcaagcc cgtgggcttc
     1741 aagctctgca tcggccatcc ctgggaattc atggcgattt gcaaagcgat gctggagacg
     1801 gggatctttg ccgacttcgt cgtcatcgac ggcacggagg gcggcaccgg cgcggcgccc
     1861 ctcgaattcg tcgaccatgt cgggacgccg ctgcgcgaag gcctggtctt cgcgcataat
     1921 tgcctcgtcg gcgccggcat ccgcgagagg ctgcgcatcg gcgcgagcgg ccgcatcgtc
     1981 acggccttcg atatggcgcg cgtcctggcg ctcggcgccg attggtgcaa ttcggcgcgc
     2041 ggcttcatgt tcgctttggg ctgtatccag tcgcaatcct gtcataccga tcgctgcccg
     2101 accggtgtcg cgacccagga tcagctgcgc cagcgcgccc tcgtggtgcc cgacaaagcc
     2161 gagcgcgtgc gccatttcca cgacaacacg ctcaaggcct tggccgagct gttgggcgcg
     2221 gccggcctct cgcatccctc cgagctccgg ccggcgcata tcctgaagcg gctgtcgccg
     2281 agcaccgtcg cgacctttgc cgaagtctat cccgggctcg ctccgggcga cctcgtcaac
     2341 ggcacggcca ccgagcgcta tcagcgatgc tgggcgctcg ccgacgcccg ctccttcgcg
     2401 ccccgcgaag acttgcgtcc ggctgcttaa atccgtcaat agtttgcgga gcgcgtggca
     2461 gaaacgcgcg tgtaacactg tgacagatga aagacggcag cctgtataaa gcgacaggtg
     2521 ccctccgcgc gatatcgtgg cccaaatacc ccgcaaacgc gcaaggcgtc agtgctgccc
     2581 gtgggattgt tgttatggaa gccgaacgcc gcccggtcac cattctcttc gccgacctcg
     2641 tcggtttcac cgccttcacg gagcgctcgg gcgaggaagc cgcctatgtc ttgatggagg
     2701 ccatccatcg gttgatggag cgagccgtcg aggcgcatgg tggcacgatc aaggaagcga
     2761 ccggcgacgg cgtcgtcgcc ctgttcggcg ttcccttcgc gctcgaggat gcgccgctca
     2821 gggcctgccg ggcggccttg tcgatccagc agtgcctcgc cgaggaggtt gacgcggtcg
     2881 cggcgggcgt cagaccgcaa ttgcgcatca gcatcaacac cggcgccgtc atcgtcgggc
     2941 cggtcgaaga gggcgcgggc ctggccgttt tcggcgacac cgtgaacacc gccgcccgcc
     3001 ttcaggcgtg cgctccgccg ggcgcgatcc ttctgagcga ggcgacccgc aagctggtcc
     3061 tgggccttgt cgattgcgag ttcgtcgggc tctgccgcct caaaggcaag gccgagccgc
     3121 agccggccta tcgcctcgat gcgatccgcc agagggtctc gcgattccag gcggtgctgg
     3181 cgcgcggtct ctcgccctat gtgggtcgtg ccggcgaacg gaagcgtctg gagggcctcc
     3241 tctgcggcgg caggcgccgt ctgcgcgtca tcgacatcgt cggcgaacca ggcatcggca
     3301 agtcgcggct gcttcacacg gttttgagcg gaaatgccga gagtgagccg attctatcgg
     3361 gcagctgttc gcccgacggc agcgcggtcc cgttcctgcc gctgatcgac gtcgtccgcg
     3421 gttgtctcgg catagccgag ggcgaagacc aggaggggat tgcgcggaag ctagccggcg
     3481 gcctcgctgc gctcgagctc ttttcggtcg cgaatgtcga tctgctcctg aatctcttgg
     3541 gcctcgcggc gtccccggag ggggcggcga tcgggctgcg cacaggcgat ctcctgcgac
     3601 ggcttctgca agcgcggtgt cgccgctctc cgctggtcgt cctgatcgag gatctgcaat
     3661 ggatcgacag cgcttcggaa gaagtgctga cgcagatcgt cgccaccacc gccgacgcgt
     3721 cgcgtctcgt cctcattcat acccgccgtc ccgagtatct gccgccctgg atcgatcacg
     3781 cgagcaccac gaccgtcccg ttgtcgtcgc tctcggccgc cgagacctcg tgcatcgcgc
     3841 aagcccgctt gggcggcggc gagctgccgg gagagttcgc gcgtctcatc gtcgagcggg
     3901 ccggcggcaa tcccctcttc gccgaggaga tagcgggctc tctgctcgag cgccgcatcg
     3961 tgcggcgggg cgcggcaggt ctgcaatatg agccgtcgat cggcgccgcc gttttgccgc
     4021 tgagcgtgca ttcgctgctg gccgctcgca tcgacggcct cgatcccagg gatcgggcgc
     4081 tgttgcgatc ggccgcggcg atcggctacc gcttcgatgc cgacatcctc ggcgcggtga
     4141 tcggcgacga tggcgggctc gaccggcggc tcgccgctct gcagcagctg ggtcttcttc
     4201 aagcgggcga cgggccccgc gcattcgtct tcaagcacgc cctggtgcgg gacgcgctct
     4261 atgacagctt gctggcagcg ccgcgtgcgg cgctgcacct caggatcgga tcggcgatcg
     4321 agcgccgcag cagcgaccgg cgcatcgagg ccgcggagat cgtcgcgggg cgctggcgca
     4381 cggcgcatgg cgccgggaag gcgtcgagcg tccgcaatcg cgccgccgcg acgcgtgccg
     4441 gcactcattg tctgaaggaa gcggagcggc atttcgcgct cgccttgcaa gtcgccgcga
     4501 catttccgtt cgggccgacg acgcgagggt cgacgtctct cgcggcgccg gtacgcgccg
     4561 tggccgtgcc ggaggaaaag actgaagaag cgcagcggaa cgggcgtgcg tccgggtcga
     4621 cctggtcgat ggccggcctc ggatggctcg acaagctcgc ctgacgcgcc gtcagctctc
     4681 gatcgtcagc tccgggccga acacgcgctc gccggagatc cagcgctcga tgatgccggg
     4741 atcgcgcagg atcgccgcga cgccctcgct cagctcgacg agatcatgct gcaggcccat
     4801 gtccttgcag atgccgcgaa tgccggcctg gtcgctgagc atgaggtatt gcgcggtcag
     4861 gaattgctcg accggaagct ccggcccgcc cctctgaatg ctctcgagcg aggcgcggca
     4921 gtccgcggaa tcggccgcga agatcgaacg gcacatcagc ggccggtcct catagaccga
     4981 gcatcggttg tcgaccagca gaggacaggg ccgaccggcg tggcggcgtt ccggcttgct
     5041 cttcgatgcc tgcgcgtcga tgcgggccaa caaagccttg cgccggggat cgccgggatc
     5101 ggcgaggtga acggcgacga ggatcgcctc ggggatcgtc acctcgacgg attgatagca
     5161 gcaataggcg cagccttccc ggcatcccag cttgggggct tcggcctgca acgcgtcgat
     5221 cgtctgctgt gcccggccga aacccaggag cttcagcagc gcgagcacgg tgcggagttt
     5281 tgtcgaggcc gaggggtcgc gggcgatttc ctgcgccgcg atcacgatgc ccgcgccacg
     5341 cgtcgtgccg gcgatctgcg cgacgagctt ctcgatctcg gcgtcgatcg gctgcggcat
     5401 ggccgggggc acgcggatgc cgatatcctc gagcgagcgg aagtaagctt cgggcgccgg
     5461 cggctgcatg gacgagcgtc ttgaaatgag ggacgatggc ccgaagctaa gcccgcagct
     5521 ttaagaaacc gctaaccaag actcgcggcg caggacaatc ccggggaggc gcagatggat
     5581 ttcggctact tcacgctcag cgacaatcac tacgccggca atcggcgggg cgccaatcag
     5641 ctcgtggccg acatcctcga cgaggtcgtt tatgccgatg cgctcggata ccattcggcc
     5701 tggatcggcg agcatcattt caattcgctg ggcgtgctct cctgccccga cctcgtgctc
     5761 gcccacgccg ccgcgcgcac gcgccacatc cggctcgcgc cggcggtcac cgtgctgccg
     5821 ctccatcatc cgatccgcgt cgccgagcaa tgggcgacgc tcgatctcct gtcgggcggc
     5881 cgcgtcgact gggcggccgg ccgcggctat gaccggcgag aatacgagcc cttcaatgtc
     5941 gatttcagcg acaaccaggc gatcttcgag gaagggatgg agctggtgcg ccggctctgg
     6001 gacgccggcg acacgcgcat ctcgcacgag ggccgctact accacttcga gaatgtgcgg
     6061 atcacgccgc agccggtgca gaaaccgatc cccgcctatg tcgcctcttt ctccaagccc
     6121 tcgatcgagc tcgccggtcg gctcggctgc ggcctcgtcg tggcgccttt cgccgccgcc
     6181 atgaccttcg ggggcttgcg ccaagtggcg gcgctctatc acgagagctg cgcgcgacat
     6241 ggcaagcggc ccggtcgcct gatgtgcagc tacttcgcgc acttcgccga cgacgcggag
     6301 caggaggcgg cggcacgcgc gcggcagatc cgctattacc gcgaatgcgt catccccgcc
     6361 ttgccgggcg atcccaagac ggcgccgccg agttatcgct acttcatcga catggtcgat
     6421 cgtctgggca aggtaacgcc ggccgacctc accgagaatt ccgtgctgct gggctcggcg
     6481 caacagatta ccgacacgct caagaaggtc gaggccgccg gcttcgacga ggtgatcctc
     6541 tatttcaatg tcggcctgaa gccgcacgcc caggtgaagg acgagatggc gcgcttcatg
     6601 acggaagtcg ctccggcctt caaacgcgcg ccggcaagag gctgaagcgc gcgctcaacg
     6661 tcgcggatcc gcacgaccgt cgatagcgga cagctgatcg cgcgaccaat cgaattgtgc
     6721 gcgcctgttc tcaagccggc accactaccg catcgagcgc gagcacgccc tctcccgccg
     6781 gcagtaccag cagcggattg atgtcgaggc tccggagcgt cggtccttgc gcggcggcga
     6841 agcgcgagag cgcggcgagc gcttgggcca gcgcggcgat gtccgccgga ggacggccgc
     6901 gcaagcccgc cagcaccgcc gcaccgcgca gctcgccgat catccgccgg gcctctgatt
     6961 cgtcgaaggg ggcgaggcgg aaggtcacgt ccttgagcag ctcgacgaaa atgccgccga
     7021 gccc
//
LOCUS       DASYNS010000185         6978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3629926, whole genome shotgun sequence.
ACCESSION   DASYNS010000185 DASYNS010000000
VERSION     DASYNS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3629926"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VGP50_05760"
     CDS             <1..690
                     /locus_tag="VGP50_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8026913.1"
                     /translation="RGRPGEVSLAHLGVLFLDELPEFQRASLEALRQPLESGKATVAR
                     VNAHVTYPARVQLVAAMNPCRCGHLDDAALACGRAPRCAQDYQSKISGPLFDRIDLHI
                     EMPAVRPADLALPPPAEGSAAVAARVAAARARQAARYQRLPPDRRIRTNAELDGQLLD
                     DLAAPDADGKRLLLEAAERLRLSARGYHRVLRVARTLADLEAADTVRRLHIAEALSYR
                     RILLGGDVRGR"
     gene            complement(892..1182)
                     /locus_tag="VGP50_05765"
     CDS             complement(892..1182)
                     /locus_tag="VGP50_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026914.1"
                     /translation="MAGPKGLSPRVVRSIFQSKEPAPATAKRYKVSANLVYLIKARRI
                     HKATTMGLKSPQMARREKRNPAAGAPIDMGRLADAISKKVVLELVRRLGGKG"
     gene            1632..2444
                     /locus_tag="VGP50_05770"
     CDS             1632..2444
                     /locus_tag="VGP50_05770"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV8026915.1"
                     /translation="MTDKVKRRLTTVLCADVHGYSRLMEADEAGTLETLHRYRAAMAG
                     LVERHDGRIVNTWGDAVIAEFASVVEAVQCAAEIQQEISSQDSDRPNAQRMRFRIGIN
                     LGDVLVDGSDIYGDGVNIAARLQELAAPGGVVISGSVYDQVHHKLSLGFDCLGRQQVK
                     NIAAPVMSYRVTTGGATTQADTVAGAGAGARSIYDAHGLSGGMRPVSDWLANLPRSIA
                     VPLVVAGFLVLINVFTGLHDIWFHWPVAVLLFIAVLRTALRRKPAGDRKEER"
     gene            complement(2596..2778)
                     /locus_tag="VGP50_05775"
     CDS             complement(2596..2778)
                     /locus_tag="VGP50_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026916.1"
                     /translation="MTNASDMSHPGIGRRAVLAMGASGAALAAAFGDAAPALAQAGPA
                     PAPGAAPSSSGSTAAC"
     gene            complement(2797..3294)
                     /locus_tag="VGP50_05780"
     CDS             complement(2797..3294)
                     /locus_tag="VGP50_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV8026917.1"
                     /translation="MTTRRNALIALLAAAIPSLPRPAHAADACLAAKSEAVALALLER
                     FVAAANAHDTSDFPNIYTESYIQHSGRSPSGLAAQIANAERLSAAFPDRRLVIEDRIV
                     GGDRLVARCTYTGTHRGPFLGFAPTGKVVTIRTIDIWRIAGGKLAEHWDVVDFAEVEK
                     QIRGT"
     gene            complement(3406..4239)
                     /locus_tag="VGP50_05785"
     CDS             complement(3406..4239)
                     /locus_tag="VGP50_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8026918.1"
                     /translation="MLQRRALLASAAALLAGAARPLLAAEPPSAPDIQRILDRGRLVV
                     AVIGSPLPPFVAAAAAGTLTGYDIELANGMARALGVPVSFDSKARSIDAVLAAVARGD
                     ADLALSKLGATLEGALRVRFSRPYLTLHQALLVNRPRFAQRAGSRDPAEVAQELGMLL
                     GVVAGTTSAEDAHRRWPRAQIRDYPRFDPELVDAVLAGELLAACGDELEARHALTTRG
                     DAPLRLRVSILPETRLGIAAALPRESLQLLAWVDLYIETALAPVTTDELLARYATKSP
                     D"
     gene            complement(4244..5026)
                     /locus_tag="VGP50_05790"
     CDS             complement(4244..5026)
                     /locus_tag="VGP50_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV8026919.1"
                     /translation="MLHTINPLYALSGFAVGMLVGMTGVGGGSLMTPLLILLFGIHPA
                     TAVGSDLLYAAATKAGGTVVHGLSRTVEWRVVGLLALGSVPMTGLTLLALSELDINSD
                     AARELITTVLSCALFFTAAVLIFRRKILSLYMEHVGQLDRRRTMLLTVATGAVLGVLV
                     SISSVGAGAIGVTALVILYPRLPTARIVGSDIAHAVPLTLLAGLGHWAVGSVDWPLVG
                     SLLAGSLPGILLGSLVSARVPDSVLRLTLAFTLIIVGSKLVF"
     gene            complement(5096..>6978)
                     /gene="cysC"
                     /locus_tag="VGP50_05795"
     CDS             complement(5096..>6978)
                     /gene="cysC"
                     /locus_tag="VGP50_05795"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEV8026920.1"
                     /translation="RTPSMIQSRAFSIVIAGHVDHGKSTLIGRLLHDTGSLPEGKVAA
                     LLEASRRRGMPFEWSFVMDALKVERDQGITIDTTRMRFKSSARDYVIIDAPGHVEFLK
                     NMLTGAAAAEAAILVVDVVDGMGEQTRRHAFLLHLLGIREIVVAVNKMDCVAYAQERF
                     EAAARAVRTYLAALDLEATAIIPISARQGDMIAHRSVPPTWYEGPTVLEALDALKGAG
                     DDLEQPLRLPVQDVYKFDERRIVVGRIEAGRLRVGDRLRFSPGERTARIASLETWSAQ
                     PMVTAVAGQSIGFTLDEDIFVERGQVASMPDAAPLVARRLKLRIFHFGQAPLMPGDAV
                     KLQIGLAEHAATIEAVERVVDVNSLTSHTAATVRRNDVADVVVASRAPIAVDDPAEGR
                     HLARGILRRGYEVIAGCLVQAALDVRQPAAALRHVVPVTSAVTPAERGAAWGHSGGVL
                     WLTGLSGAGKSTLARALEKELFRRHWRAVLLDGDTLRHGLNVDLGFSDHDRAENVRRI
                     GAMARLLAENGMIAIVACIAPRAAHREALRSDLGSLYHEVYVKAPLAVCERRDVKGLY
                     AKARRGEIAGFTGLSDPYEPPQAPALEISTDVLTPAECLEHLVAYIDRALSASDVRRM
                     AS"
BASE COUNT         1192 a         2306 c         2382 g         1098 t
ORIGIN      
        1 cgcggccggc ccggcgaggt gtcgctcgcg catctgggcg tgctgtttct cgatgagctg
       61 ccggagttcc agcgcgccag cctcgaggcg ctgcgccagc cgctcgaaag cggcaaggcg
      121 acagtggcgc gggtcaacgc ccatgtgacc tatcccgccc gcgtgcagct cgtcgcggcg
      181 atgaacccgt gccgctgcgg ccatctcgac gacgcggcgc tcgcctgcgg ccgcgctccc
      241 cgatgcgcgc aggactatca atcgaagatc tctggcccgc tcttcgaccg catcgatctc
      301 cacatcgaga tgccggccgt gcggcccgcc gatctcgcgc tgccgccgcc cgccgaaggc
      361 tcggccgcgg tcgcggcacg cgtcgcggcg gcgcgcgccc ggcaagcggc gcgctatcag
      421 cggcttccgc ccgaccgccg catccgaacc aatgccgagc tcgatggcca gctcctcgac
      481 gatctggcgg cgccagacgc ggatggcaag cgactgctcc tggaggccgc cgagcggctc
      541 cgtctttccg cgcgcggcta tcaccgcgtg ctgcgcgtcg cccgcacgct cgccgacctc
      601 gaggccgccg acacggtgcg acggctccac atcgccgaag cactgagcta ccgccgcatc
      661 ctgctcggcg gcgacgtgcg gggacggtga gaggccggcg ggcgagcagc gtagcaacgc
      721 cgtgaagccg aagaccgtcc cgtcaagcgc tcccgcgcga gatgatccga agcacctgat
      781 cgcacaagat caacaaagaa ttttgtttga tgcgagtccg gtttgcgggt cggtcgagcg
      841 cgtcgactct cagatcccca ccccttttct agtcctcaac cacggtggtg gtcacccctt
      901 cccacccagc cgccttacga gctccaggac aacttttttc gatatcgcat cggcgagacg
      961 gcccatgtcg atcggcgcgc cggctgccgg gtttcgcttc tcccgtctgg ccatctgtgg
     1021 gctcttcaac cccattgtgg tggccttgtg gatacggcgc gctttgatca ggtagacgag
     1081 gttggcggaa accttgtagc gcttcgccgt cgccggagcg ggctccttcg attgaaagat
     1141 gctgcggacg acgcggggtg agagcccctt tggacctgcc atagcgatgc tccttcgacg
     1201 tgcgggagga tctgagcaat tcattcctgc cggaaattgg aattgttgac ctctcccagt
     1261 taatcacgcg ctaaatactc tgacagcgct ccgatggcaa cgtttttgac gctgggatca
     1321 gattttgaac gtccttgcgc gaaaaaaccg ggcggctttc tattagaaga ttacggacag
     1381 ctcgcaattg ttcattcata aagcagcgcg tttcatcaaa gtcttgacga tgatcggatg
     1441 aatctgcccg actggcacca tgtaagctcg acagagagtg ttgtgcacct cgaccacggt
     1501 agaaacggcg tggaatggca ggccgtcgcg cgtaaacttt agtacagaga cgcgcacgtc
     1561 gagatacttg tcatcaatcc cgagaatgag ttcggtatcg ctgatcggtt tacggggcta
     1621 ctatgtggcc catgaccgac aaggtgaaac ggcggctgac caccgtcctg tgcgccgatg
     1681 tgcacggata ttcccgtctt atggaagcag acgaagcggg cacgctcgag acgctgcacc
     1741 ggtaccgcgc cgccatggcg ggattggtgg agcgccatga tgggcggatc gtgaatacct
     1801 ggggtgacgc cgtcatcgcc gagttcgcca gcgtcgtcga ggccgtgcag tgcgcggccg
     1861 agatccagca ggaaatttcc agccaggatt cagaccggcc caatgcgcag cgcatgcggt
     1921 ttcgcatcgg catcaatctg ggcgatgtgt tagtggacgg ttccgacatc tatggcgacg
     1981 gcgtcaatat cgccgcgcga ctgcaagaac ttgcggctcc tggcggcgtc gtgatctccg
     2041 gttccgtcta cgatcaggtg caccacaaac tgtcgcttgg cttcgactgt cttggccggc
     2101 agcaggtaaa gaacattgcg gctcccgtca tgagctatcg ggttaccacc ggcggcgcca
     2161 cgacgcaggc ggatacggtt gccggcgccg gggctggcgc ccgatcgatt tatgacgctc
     2221 acggcctgtc cggggggatg cgacctgtct cggattggtt ggcgaacctg ccgcgctcca
     2281 ttgcagtgcc tctcgtcgtc gcgggctttc tggttctgat caatgtattc accgggctgc
     2341 acgacatctg gtttcattgg ccggtggcgg tcctgctgtt catcgcagtc ctgcggacgg
     2401 cgctccgccg caagcctgca ggggaccgaa aggaagagcg ctgaacgagc ggcgcgtgag
     2461 atggcccggt ccgtgcgaac gccgtgacgg gagcgccacg cgctccgccg cttgcgatcg
     2521 aattcgcttt agccgtcgac ggcggaaagc gagcgcgccg acaaggacga tctccgcccg
     2581 cgagcgagag gatcgtcagc acgctgccgt cgagccgctc gacgacggcg cggcgccagg
     2641 cgccggcgcg ggtccggcct gagcgagcgc cggagccgca tcaccgaaag cggcggcgag
     2701 cgcggccccg ctcgcgccca tcgccagcac cgcgcggcgt ccgatgcccg gatgcgacat
     2761 gtccgatgcg ttcgtcatcc cttgcctcct tcggcgctaa gtgccgcgta tctgcttttc
     2821 gacctcggcg aagtcgacga cgtcccagtg ctcggcgagc ttgccgcctg cgatgcgcca
     2881 gatgtcgatg gttctgatcg tcaccacctt gccggtgggc gcgaagccga gaaacgggcc
     2941 gcgatgggtg cccgtgtagg tgcagcgcgc gacgagccgg tcgccgccga cgatgcggtc
     3001 ctcgatgacg agacgccgat cgggaaacgc cgcgctcagg cgttcggcgt tggcgatctg
     3061 ggctgcaaga cccgacgggc tgcgaccgga atgctgaatg tagctctccg tgtagatgtt
     3121 cggaaagtcg gacgtgtcgt gcgcattcgc ggcggcgacg aaacgctcga ggagcgcgag
     3181 cgcaacggct tcggatttgg ccgcgagaca cgcatccgca gcatgcgccg ggcgtggcag
     3241 cgacgggatc gcggcggcta acagggcgat gagcgcattt cgccgggtgg tcattggctc
     3301 tcctccgatc gtcgagacct cgtgaggcta tgcctagtgc tgctatgggt caagacccgt
     3361 cgcgaaaccg gtgccatcgc cggacgggca aagcatccaa cacgtctaat cgggtgactt
     3421 ggtggcgtaa cgtgcgagca actcgtcggt cgtgaccggc gcgagtgcgg tctcgatgta
     3481 gaggtcgacc caggcgagga gctgaaggct ttcgcgcggc agcgctgcgg caatgccgag
     3541 tcgcgtttcc ggcaggatcg acacgcgcag ccggagcggc gcgtcgccgc gcgtggtgag
     3601 agcatggcgc gcttcgagct cgtcgccgca ggcggcgagc agctcacccg cgagcacggc
     3661 gtccacgagc tcgggatcga agcgcgggta gtcgcgaatt tgcgcccgcg gccagcgacg
     3721 gtgcgcatcc tcggccgaag tcgtgccggc gaccacgccg agcagcatgc caagctcttg
     3781 cgccacttcg gccggatcgc ggctgccggc acgctgggca aagcgcggcc ggttgaccag
     3841 gagcgcctgg tgcagcgtca gataggggcg cgagaagcgc acgcgcagcg ctccctcgag
     3901 cgtcgcgccg agcttcgaga gcgcgagatc cgcatcgccg cgcgccaccg ccgcaaggac
     3961 ggcatcgatg ctccgcgctt tgctgtcgaa gctgaccggc acaccgaggg cgcgcgccat
     4021 gccgttggcg agttcgatgt cgtagccggt cagcgtgccc gcggcagcag cggcaacgaa
     4081 aggcggcaac ggagagccga taacggcgac gacgagccgg ccgcgatcga ggatgcgctg
     4141 gatgtcgggt gcggacggcg gctccgcagc caggagcggc cgcgcggctc cggcgagaag
     4201 cgccgcggcc gaagcgagaa gcgcgcgccg ctgcagcatc gcctcagaag acgagcttgc
     4261 tgccgacgat gatgagggta aaggcgaggg tgaggcgcag cacgctgtcg gggacgcggg
     4321 ccgacaccag gctgccgagc aggatgccgg gcaaggaacc ggcgagaagc gacccgacca
     4381 ggggccagtc gaccgacccc accgcccaat ggccgagccc ggccaacagc gtcagcggca
     4441 cggcatgggc gatgtcggag ccgacgatgc gcgccgtcgg cagccgcgga tagaggatca
     4501 ccagcgccgt gacgccgatg gcgcccgcgc ccaccgagga gatcgacacc agcacgccga
     4561 gcaccgcgcc cgtggcgacg gtgagcaaca tcgtgcggcg acgatcgagc tggccgacat
     4621 gctccatgta gagtgacagg atcttgcggc ggaagatgag aactgctgcg gtgaagaaca
     4681 acgcgcagct gagcacggtc gtgatcagct cgcgcgccgc gtcgctgttg atgtcgagct
     4741 cggagagagc gagcagcgtc agtccggtca tcggcacgct gccgagcgcg agcaagccga
     4801 cgacgcgcca ctcgacggtc cggctcaggc catggaccac cgtgcctccc gccttggtcg
     4861 cggcggcgta aaggaggtcg ctgccgacgg cggtcgccgg gtgaatgccg aagagcagga
     4921 tgagcagcgg cgtcatcagc gatccgccgc cgacgccggt catccccacc agcatgccga
     4981 ccgcgaagcc cgagagcgcg tagagcgggt tgatcgtgtg cagcacgttc ctgatcccgg
     5041 tttggcgccg ctcgaagtta ggggatgagc cgcggtagag gaaggtgatt caacctcaag
     5101 atgccatccg gcggacgtca ctcgcgctca gagcgcggtc gatataagcg acgagatgct
     5161 cgaggcactc ggccggcgtc agcacatccg tgctgatctc cagcgccggc gcttgcggcg
     5221 gctcgtaggg atcggacaat cccgtgaagc cggcgatctc gccgcgccgc gccttggcat
     5281 agagcccctt cacatcgcgc cgctcgcaga cggcaagcgg cgccttgacg tagacctcat
     5341 gatacaacga accgaggtcg ctgcgtaggg cttcgcgatg ggctgcacgc ggggcgatgc
     5401 aggccacgat ggcgatcatg ccgttctcgg ccaaaagccg cgccatcgcg ccgatgcgcc
     5461 gaacattctc ggcccggtca tggtcggaga aaccgaggtc gacattgagg ccatggcgca
     5521 gggtgtcgcc gtcgaggagc acggcgcgcc aatggcggcg gaacagctcc ttctcgagcg
     5581 cgcgcgccag cgtcgacttg ccggcgcccg aaagcccggt gagccacagc acgccgcccg
     5641 aatggcccca cgcggcgccg cgctcggcgg gcgtcaccgc ggaggtcacc ggcaccacgt
     5701 ggcgcagcgc cgccgccggc tggcgcacat cgagcgccgc ctggacgagg cagccggcga
     5761 tgacctcata gccgcgccgc aggatgccgc gcgcgagatg ccgtccttcc gccggatcgt
     5821 cgacggcgat cggcgcgcgg ctcgccacga cgacgtcggc gacgtcgttg cgccgaaccg
     5881 ttgccgcggt gtggctggtc aggctgttga cgtcgacgac gcgctcgacc gcctcgatgg
     5941 tcgccgcgtg ctcggcaagg ccgatctgca gcttgaccgc atcgccgggc atgagcggcg
     6001 cctgcccgaa atggaagatc cggagcttca ggcgccgcgc cacgagcggc gccgcatcgg
     6061 gcatgcttgc cacctgcccg cgctcgacga agatgtcctc atcgagcgtg aagccgatcg
     6121 actgcccggc gacggccgtg accatgggct gcgcgctcca ggtctcgagc gaggcgatcc
     6181 gcgccgtgcg ctcgccgggc gagaagcgca agcggtcgcc caccctgagc cgacccgctt
     6241 cgatccgacc gacgacgatc cgccgctcgt cgaatttgta aacgtcctgc acgggcaggc
     6301 gcagcggctg ctcgagatcg tcgcccgcgc ccttgagcgc gtcgagcgcc tcgagcacgg
     6361 tcgggccttc ataccaagtg ggcggcaccg agcggtgagc gatcatgtcg ccctggcgcg
     6421 ccgagatcgg aatgatcgcc gtcgcctcga gatcgagggc ggcgagatat gtccgcaccg
     6481 cgcgcgccgc cgcctcgaag cgttcctgcg cataggcgac gcaatccatc ttgttgacag
     6541 cgacgacgat ctcgcggatg ccgaggaggt gcagcaggaa ggcatggcgg cgcgtctgct
     6601 cgcccatgcc gtccaccacg tcaaccacca ggatcgccgc ctcggcggcg gccgcgccgg
     6661 tcagcatgtt cttgagaaac tcgacatgcc ccggcgcatc gatgatcaca taatcgcgcg
     6721 ccgagctttt gaagcgcatg cgcgtcgtgt cgatcgtgat cccctggtcg cgctccacct
     6781 tgagcgcgtc catgacgaag gaccattcga agggcatgcc gcgccgccgg ctcgcctcca
     6841 gcagcgctgc caccttgccc tccgggagcg aacccgtgtc gtgcaagaga cggccgatca
     6901 gcgtcgactt gccgtgatcg acatggccgg cgatgacgat ggagaaggcg cggctttgga
     6961 tcatcgacgg cgtcctca
//
LOCUS       DASYNS010000186         1571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1445175, whole genome shotgun sequence.
ACCESSION   DASYNS010000186 DASYNS010000000
VERSION     DASYNS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1445175"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VGP50_05800"
     CDS             complement(<1..276)
                     /locus_tag="VGP50_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026921.1"
                     /translation="MLRSFIRRWRHRLDFLARETSYQARGLQSLVAALAFAPADGPPI
                     LVLVAAPAQARYLARWSRWLCRAPCETRVIADLAQLERSLTEAAPPRR"
     gene            complement(277..>1571)
                     /locus_tag="VGP50_05805"
     CDS             complement(277..>1571)
                     /locus_tag="VGP50_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV8026922.1"
                     /translation="MRFVKRRSRPGARLSLILLDWSVRESFHLLHYLRQQTVPRDAFE
                     VIVVEYYDRESPALQRFAEEVDTWLLLEMPRDCLYHKHLMYNAGIAVAGGEILMIGDS
                     DAMVRPSFIAAILDAFAKDPRQVLHLDQFRNHRRDFYPFSYPSFEEVLGDGCINNVAG
                     KTAGVLDTLDPIHRRNYGACMCARRADLIAIGGADEHADYLGHICGPYDMTFRLVNRG
                     EREVWHESEFMSHTWHPGQAGEDNYQGPHDGRQLSTTSIEALVSRRVMPLVENAAIAS
                     LRRGEGLSETALLERVIDPAMVRLWQSVSHGGSAPPVASDRLVYQGHLIEHGRAGWTA
                     RPILAAQPTREARSLPALKAMIDRAAPSSLRIFTAWAAGFVLLDRLWERFVLDRRRTA
                     PPPLAPRAPAAPRSSPSLPLEGEGRVGGSAASLPARRR"
BASE COUNT          256 a          479 c          606 g          230 t
ORIGIN      
        1 gcggcgcggc ggggcggctt cggtgaggga gcgctcgagc tgcgccaggt cggcgatcac
       61 gcgcgtctcg cacggcgcgc ggcagagcca gcgggaccag cgggcgagat agcgcgcttg
      121 ggcgggggct gccaccagca cgaggatcgg cgggccatcg gcgggcgcga aggcgagggc
      181 ggcgacgagg ctctgtagcc cgcgcgcctg atacgatgtt tcgcgcgcca ggaagtcgag
      241 gcggtgtcgc catcttcgga tgaaagagcg cagcatctag cggcgccttg cgggcagcga
      301 ggcggcacta cccccgaccc gtccctctcc ctcgaggggg agggaggggg aagatcgggg
      361 tgcggcggga gcgcgcggcg ctagcggcgg tggtgccgtg cggcggcggt cgaggacgaa
      421 gcgctcccag aggcggtcga ggaggacgaa gccggcggcc caggcggtga agatgcggag
      481 gctcgagggc gcagcacggt cgatcatcgc cttgagcgcg ggaagcgacc gggcttcacg
      541 tgtcggttgc gcggcaagaa tcgggcgcgc ggtccagccg gctctgccgt gctcgatcag
      601 gtggccctga tagacgagac ggtcggacgc caccggcggc gcgctgccgc catggctgac
      661 cgattgccag agccgcacca tcgccggatc gatgacgcgc tcgaggagcg ccgtttccga
      721 caatccctcg ccgcggcgca ggctggcgat ggcggcgttc tcgaccagcg gcatcacgcg
      781 gcgcgacacc agcgcctcga tcgaggtggt cgagagctga cggccgtcat gggggccttg
      841 gtaattgtcc tcgccggcct ggcccgggtg ccaggtgtgc gacatgaatt cgctctcgtg
      901 ccagacctcg cgctcgccgc gattgacgag gcggaaggtc atgtcatagg ggccgcagat
      961 atggccgaga taatcggcat gctcgtcggc gccgccgatg gcgatgagat cggcacggcg
     1021 cgcgcacatg caggcgccgt agttgcggcg atggatcggg tcgagggtat cgagcacgcc
     1081 cgcggtcttg ccggcgacat tgttgatgca gccgtcgccc agcacctcct cgaagctcgg
     1141 gtagctgaag ggatagaagt cgcggcggtg gttgcggaac tggtcgagat gcagcacctg
     1201 gcgcgggtcc ttcgcgaagg cgtcgaggat ggccgcgatg aagctcggcc gcaccatcgc
     1261 gtccgaatcg ccgatcatca ggatctcgcc gcccgcgacg gcgatgccgg cattgtacat
     1321 gaggtgcttg tgatagaggc agtcgcgcgg catctcgagc aggagccagg tgtcgacctc
     1381 ttcggcgaaa cgctggagtg ccggcgattc ccggtcgtaa tactcgacga cgatcacctc
     1441 gaaggcgtca cgcggcactg tctgctggcg caggtaatgg agcagatgga agctctcgcg
     1501 cacgctccaa tcgaggagaa tgaggctgag ccgggctccg gggcgcgagc gccgcttgac
     1561 gaatctcatg c
//
LOCUS       DASYNS010000187         4210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_509278, whole genome shotgun sequence.
ACCESSION   DASYNS010000187 DASYNS010000000
VERSION     DASYNS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509278"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VGP50_05810"
     CDS             <1..314
                     /locus_tag="VGP50_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026923.1"
                     /translation="EHPLKKIAALDRSTEAKLGIPLARHRSVILVKRSTYRKLERLPT
                     KGNRCLHIDAYHIVIPAKAGTQSERRAGGPWVPAFAGMTKEEWDTESHLRSSRFRFVA
                     K"
     gene            509..940
                     /locus_tag="VGP50_05815"
     CDS             509..940
                     /locus_tag="VGP50_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8026924.1"
                     /translation="MARILVVEDSNDVQELLCLALADEGHAAHGVETVAQGSRALEGG
                     NYELLIADIRLPDGSGAELADRALTRGVEALLTTGHPEEMQILERRGIDYLRKPFPLK
                     YMMRLVDRLVAKGRLRRARAAASRLLPPRRRDHSGSVKRAP"
     gene            complement(953..1486)
                     /locus_tag="VGP50_05820"
     CDS             complement(953..1486)
                     /locus_tag="VGP50_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV8026925.1"
                     /translation="MTIDAIRDALPDYAKDLRLNFGSLLDDSVLSEQQRWGALLATSL
                     AARNARVIAAVSAEAARRLSPAAQTAAKAAAAVMAMNNIYYRFTHLASDKEYATMPAR
                     LRMNVIANPGVPKADFELWSLAVSAVNGCGACIDAHDQVLRKAGVSREAVQQTVRIAA
                     VIHAVAATLDGEESLAA"
     gene            complement(1510..2049)
                     /locus_tag="VGP50_05825"
     CDS             complement(1510..2049)
                     /locus_tag="VGP50_05825"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV8026926.1"
                     /translation="MLTVGDKLPAFALTAVSGTDPRSAFTPATNESYAGKWLVLFAWP
                     KDFTFVCPTEIVGFDDLGREFRDRDAQLLGLSIDSEFVHLAWRQSREDLGKVSFPWLS
                     DIKRELSAALGILDKDAGVALRATFIVDPQGVIRFAAVNDLSVGRNPREVLRVLDALQ
                     TDELCPCNWQKGEDTLQAA"
     gene            2148..3056
                     /locus_tag="VGP50_05830"
     CDS             2148..3056
                     /locus_tag="VGP50_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEV8026927.1"
                     /translation="MNALPSLQQLRFLAALADEKHFGRAAASCAVTQSTLSAGIQELE
                     ERLGASLVERSRRSVLLTPLGHDIAARGRRLLRDAQDLAETAQAGREPLAGPLRLGVI
                     PTIGPYLMPAAMTGLAKAFPKLKLYLREEQTAPLLEKLAKGQLDVALIALPYDMGALE
                     TMTLGDDRILVALPKSHPLARARRIARAALVGEELLLMEDGHCLRSHALAACRLVGPD
                     RNEVFQGTSLRTLLQMAAGGIGITLLPEMAVKPELAAAPGLVARPLEGAPARGIALAW
                     RPTSARKSEFRTLGRYLKTVLDASSA"
     gene            complement(3362..3826)
                     /locus_tag="VGP50_05835"
     CDS             complement(3362..3826)
                     /locus_tag="VGP50_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV8026928.1"
                     /translation="MAASVKPIPEGYHTVTPYLIFDRAADAIAFYKKAFGATEIMRMD
                     APGGRIGHAEIKLGDSAIMLADEHPEQGALSPRKLGGSPVMLHVYVEDVDAMVERAVA
                     AGAKLIRPIADQFYGDRTGGLEDPFGYRWFIATHKEDLTADEIRRRAAAQGQ"
BASE COUNT          699 a         1430 c         1390 g          691 t
ORIGIN      
        1 atgagcatcc cttgaagaaa atcgcggcgc tcgatcggtc gaccgaggcc aagctcggca
       61 tcccgcttgc gcgtcatcgg agcgtcattc ttgtaaagcg aagcacatac cggaaattag
      121 agcgcctccc gaccaaaggg aatcgatgcc tccacatcga tgcctaccat atcgtcatcc
      181 ccgcgaaagc ggggacccag agcgagcgac gtgccggtgg cccctgggtc cccgctttcg
      241 cggggatgac gaaagaagaa tgggacacgg agagtcacct tcgctcatcg cgtttccgtt
      301 ttgtagcaaa ataggggcga ccggaagcag catgagaaac gtccggcggg ttggcaatgc
      361 gatggcgtgc cgatctgctc aaaaggtgag cgctcgtccc tcatcactag atttttactt
      421 cgtcttctct tgcccgctct gccgtcgtcg gggtcagata agcgtgtcgc actttctgca
      481 atcggagtac cggctcggga ggtattctgt ggcacgcatt ctcgtcgtgg aagactcgaa
      541 cgacgtccag gagcttcttt gcctcgcgct ggcggatgag ggtcatgcgg cgcacggcgt
      601 cgaaaccgtg gcccaaggca gccgcgctct cgagggcggg aattacgagc tgctgattgc
      661 cgatatccgg ctgcccgacg gttcgggcgc cgagctcgcg gacagggcgc tcacgcgcgg
      721 cgtcgaagcg cttctgacca cgggacatcc ggaagaaatg cagatcctgg agcggcgcgg
      781 gatcgactat ctgcgcaagc ccttcccgct gaaatatatg atgcggctgg tggaccggct
      841 ggtggcaaag ggccggctca ggcgggcgcg cgccgccgct tcccggctgc tgccgccacg
      901 gcggcgtgac cattccggca gtgtgaagcg cgcgccgtag cggctccgct gctcaagccg
      961 cgaggctctc ctcgccgtcg agagtcgcgg ccacggcgtg gatcaccgcg gcgatgcgca
     1021 ccgtctgctg gaccgcctcg cgcgagacgc cggccttgcg caggacctgg tcatgggcgt
     1081 cgatgcaggc gccgcagcca ttcaccgccg agaccgccag cgaccagagc tcgaaatcgg
     1141 ctttgggcac gccgggattg gcgatgacgt tcatgcgcag ccgcgccggc atcgtcgcat
     1201 attccttgtc cgaggcgaga tgcgtgaagc ggtaatagat gttgttcatc gccatcaccg
     1261 ccgccgcggc cttggcggca gtctgcgcgg ccggcgagag gcggcgtgcc gcctctgccg
     1321 agaccgcggc gatgacgcgc gcgttgcggg cggcgagcga agtcgccagc aacgcgcccc
     1381 agcgctgctg ctcgctcagc acgctgtcgt cgagcagcga gccgaaattg agcctcagat
     1441 ccttggcata gtcgggcagc gcgtcgcgga tcgcatcgat cgtcatgggg tgctccttgt
     1501 catgccggct caggcggcct gaagcgtgtc ctcgcccttc tgccagttgc aggggcagag
     1561 ctcgtccgtc tgcagcgcgt cgagcacgcg cagcacctcg cgcgggttgc ggccgacgct
     1621 caggtcgttc accgcggcga agcggatgac gccctgggga tcgacgatga aggtggcgcg
     1681 gagcgcgacg cccgcgtcct tatcgagaat gcccagcgcc gccgaaagct cgcgcttgat
     1741 gtccgagagc cagggaaaac tcaccttgcc cagatcctcg cggctctgcc gccaggcgag
     1801 atgaacgaac tcgctgtcga tcgagaggcc caggagctgg gcgtcgcggt cgcggaactc
     1861 gcgcccgaga tcgtcgaatc cgacgatctc cgtcgggcag acgaaggtga aatccttggg
     1921 ccaggcgaag agcacgagcc attttccggc atagctctcg ttggtcgcag gcgtgaaggc
     1981 cgagcgcgga tcggtgccgg acacggccgt aagggcgaag gcaggaagct tgtcgccgac
     2041 ggtcagcatt gctggttctc cttgcggggg ttgtcttgac gcgaatatgg ggccatatgc
     2101 accatcgata aaacgaatta ttttcgccga cgtgatcgga aatatcgatg aacgctttgc
     2161 ccagcctgca gcagctccgc ttcctcgcgg ccctcgccga cgagaagcat ttcggccgcg
     2221 ccgccgcttc ctgcgcggtc acccagtcga cgctgagcgc cggcatccag gagctcgagg
     2281 agcggctcgg cgcgagcctc gtcgagcgca gccgccgcag cgtgctgctg acgccgctcg
     2341 gccacgacat cgccgcgcgc ggccgccggc tcttgcgcga cgcgcaggat ctggccgaga
     2401 cggcgcaagc cggccgcgag ccgctcgccg ggccgttgcg cctcggcgtc attccgacga
     2461 tcggcccgta tctcatgccg gccgccatga ccggcctcgc caaagccttc ccgaagctca
     2521 agctctatct gcgcgaggag cagacggcgc cgctcctcga gaagctggcg aaaggtcagc
     2581 tcgacgtcgc gctgatcgcg ctgccttacg acatgggcgc gctcgagacg atgacgctcg
     2641 gcgacgaccg cattctcgtg gcgctgccca agagccatcc gctcgcgcgc gcgcgccgca
     2701 tcgcgcgcgc ggcactcgtc ggcgaggagc tcttgctcat ggaggacggg cattgcctgc
     2761 gcagccacgc gctcgccgcc tgccgcctcg tcggccccga tcgcaacgag gtgttccagg
     2821 gcacgagcct ccgcaccctc ttgcagatgg cggcgggcgg catcggcatc acgctcttgc
     2881 cggagatggc ggtgaagccc gagctcgccg ccgcgccggg cctcgtcgcg cggccgctcg
     2941 aaggcgcgcc cgcgcgcggc atcgccctcg cctggcgccc gacctcggcc cgcaaatccg
     3001 aattccgcac cctcggccgc tacctcaaaa cggtcctcga cgcatcatcc gcctaacatt
     3061 ccctctcccg catcgcgcca ggcattcaca catcacgcat cactccctct ccgcccctca
     3121 ggggcggaga gggctggggt gaggtggggg cgctgccaca aatttggaat gctgatgcac
     3181 tggtcgggaa caagattccc gacgggagca tcgatatcga ctgcgttctg cagcgctcgg
     3241 gcgcgacgcc ccccacctca ccctgaccct ctccgcccct gaggggcgga gagggaaccg
     3301 ctttgccgga tgcgtgaatc ccccgagcgc gatgccggag agagaatgtc aggcacgcgg
     3361 cctactgtcc ctgcgcggca gcgcggcggc ggatctcgtc ggccgtgaga tcttccttgt
     3421 gtgtcgcgat gaaccagcga tagccgaagg ggtcctcgag accgccggtg cggtcgccgt
     3481 agaattgatc ggcgatgggg cggatgagtt tcgcgcccgc ggcgaccgcg cgctcgacca
     3541 tcgcgtccac atcctcgaca tagacatgca gcatgacggg cgagccgccg agcttgcgcg
     3601 ggctgagcgc gccctgctcg ggatgctcgt cggcgagcat gatggctgaa tcgccgagct
     3661 tgatctcggc atggccgatg cggccgccgg gtgcatccat gcgcatgatc tccgtcgcac
     3721 cgaatgcttt cttgtagaaa gcgatcgcgt cggcggcgcg atcgaaaatg agatagggcg
     3781 tgaccgtgtg atagccctcg ggaatcggtt tgacggacgc ggccatgatc agctcctcgc
     3841 ggatgtctcg ctgcaagaca tggaacgccg cggaatgcgg gacaagcggc gcggcacgaa
     3901 gaaaacgccc gcgcgttcgg cgaatctcgc cacggaaacg atgccggaac cgccgcaaat
     3961 cacccgactt ccgcgcgaat ttcgtcgtcc gcttgcccac ttgccggaga cccccgcggc
     4021 gagtatgtac gaacgtgtga ccgccacagc ccccttccgc atggccctcc cgacgcagct
     4081 cgcccactcc tccggcaagg tgccggtccg cgtccgggtg agccgccgcg cccgccgcct
     4141 ggccttgcgc atcgacgccg tggccgacgc cgtcgagctg gtcctgccgc cgcgcacctc
     4201 cctcccgcgg
//
LOCUS       DASYNS010000188         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1602871, whole genome shotgun sequence.
ACCESSION   DASYNS010000188 DASYNS010000000
VERSION     DASYNS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602871"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VGP50_05840"
     CDS             complement(<1..414)
                     /locus_tag="VGP50_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8026929.1"
                     /translation="MRGKIDRKTSRRALLAGAAATGALAFLPARFAIGQAAKVKLGLM
                     LPYTGTYASLGKNIDEALRLAIAEAGGTLGGRPVEYVVLDDESEPSKGAENANKLVAR
                     DKVDFLIGTVHSGVAMAMVKVAGDNDTMLIIPNAGA"
     gene            complement(467..2053)
                     /locus_tag="VGP50_05845"
     CDS             complement(467..2053)
                     /locus_tag="VGP50_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEV8026930.1"
                     /translation="MSADGSASEVPEIAIPRDYNAAVDLIERNLKAGRGGKAAFRDDR
                     GAYTYAVLAERVDRAANALRGLGLAPEQRILLCLLDGIDFPTMFLGAIKAGIVPIAVN
                     TLLSPADYDFMLRDSRARALFVSDALLERFAPVLARHPSLAHVVVSGAAKSEGHRALS
                     DLLAAAAPRAEAAATTADDICFWLYSSGSTGAPKGVVHLHSHLILTAELYAKPILGIT
                     EKDVVFSASKLFFAYGLGNSLTFPMSVGATAILMAERASPAAVFQRLKQHRPTIFYGA
                     PTLYAAMLASPELPKRGEVALRLCSSAAEPLPENIGDRWTEHFGVEILDGIGSTEMLH
                     IYLSNRAGAVRYGTTGTPVPGYQIRLVDDEGNPVPQGEMGELQVAGPTSAAFYWNNRE
                     GSRRTFLGPWTRSGDKYVEGNDGYFTYCGRTDDMLKVSGMYVSPIEVENALIAHPGVL
                     EAAVVGQLDEQKLAKPKAYIVLNPGVAASPALALELQEHVKSRLAHFKYPRWIEFIEE
                     MPKTATGKIQRFKLRARGTL"
     gene            complement(2167..>3100)
                     /locus_tag="VGP50_05850"
     CDS             complement(2167..>3100)
                     /locus_tag="VGP50_05850"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV8026931.1"
                     /translation="LGVVGVFVFFIISGFLVTESFEATASPLRFLAKRALRIYPGLIL
                     CILVCAVVLGPLATTLPLDAYFASGGLYDFVFSNIMLNVEHNGLPGVHFTGFDIGGIV
                     DGPLWSLPCEVVMYLLVLVLGSMRLLRLSVLWPLLALGLVCLSLDTAASPYFIGTVGW
                     LLAFFVAGMILYKLRDRHIFDGRIALLALAGLLMSIPLGRFLLLFPLFGAYLVLYLAL
                     DRRLPVLRAARFGDLSYGLYIYGWPVQQGLLYASGGRLLWWQLFPLALAIAAALAFLS
                     WHLVEQPALRLKPRGQGLAAGGGYAAPVLPVAPR"
BASE COUNT          553 a          995 c         1080 g          472 t
ORIGIN      
        1 cgcgccggca ttggggatga tcagcatggt gtcgttgtcg cccgccactt tgaccatcgc
       61 catggcgacg cccgaatgca ccgtgccgat caggaaatcg accttgtcgc gcgccaccag
      121 cttgttggcg ttctcggcgc ctttcgacgg ctccgattca tcgtcgagaa cgacatactc
      181 gaccgggcgg ccgcccagcg tgccgccggc ctcggcaatg gcgagtcgca gcgcctcgtc
      241 gatgttcttg ccgagcgaag cataggttcc ggtgtaaggc agcatcagtc cgagcttcac
      301 tttcgctgcc tggccgatgg cgaagcgcgc cggcaggaac gccagcgccc ccgtggcggc
      361 agcgcccgcg agcagcgcgc ggcggctcgt cttccgatcg atctttcccc tcatgacgtc
      421 cctccccctt ttggtctccg tgcctctctg gtcaatcttg atatccctag agcgtgccgc
      481 gcgcgcgcag cttgaagcgc tggatcttgc cggtggcggt tttcggcatc tcctcaatga
      541 actcgatcca gcgcggatat ttgaaatgcg cgagccgcga cttcacatgc tcctgtagct
      601 ccagcgcgag cgccggcgac gctgcgacgc ccggattcaa gacgatatag gccttgggct
      661 tcgcgagctt ctgctcgtcg agctggccga cgacggcggc ttcgagcacg ccgggatgag
      721 cgatgagcgc gttctcgacc tcgatcggtg agacatacat gccgctcacc ttcagcatgt
      781 cgtcggtccg cccgcaatag gtgaaatagc cgtcgttccc ctcgacatat ttgtcgccgc
      841 tgcgcgtcca ggggccgagg aaggtgcggc ggctgccctc gcggttgttc cagtagaagg
      901 cggcgctggt cggtcccgcg acctggagct cgcccatctc gccttgcggg acgggatttc
      961 cctcatcgtc gacgaggcgg atctggtagc ccggcaccgg cgttcccgtc gtgccgtagc
     1021 gcaccgcgcc ggcgcggttc gagagataga tgtgcagcat ctcggtcgag ccgatgccgt
     1081 cgaggatctc gacgccgaaa tgttcggtcc agcggtcgcc gatattttcc ggtagcggct
     1141 cggccgccga actgcagagc ctgagcgcca cctcgccgcg cttcggcaac tcggggctgg
     1201 ccagcatggc ggcgtagagc gtcggcgcgc cgtagaagat cgtcggccgg tgctgcttca
     1261 ggcgctgaaa caccgcggcc gggctcgcgc gttccgccat gaggatcgcc gtcgcgccca
     1321 ccgacatcgg gaaggtcagc gaattgccga ggccataggc gaagaacagc ttcgaggccg
     1381 agaagacgac gtccttctcg gtgatcccga ggatcggctt ggcatagagc tcggcggtga
     1441 ggatgagatg cgagtggaga tgcacgacac ccttgggcgc tccggtcgag cccgaggaat
     1501 agagccagaa gcagatgtcg tctgcggtcg tggcggcggc ttcggcgcgc ggcgcggccg
     1561 ctgcaagcag atccgacagc gcccggtgcc cttcgctctt cgccgcgccg gagaccacga
     1621 catgggcgag cgaagggtgg cgcgccagca ccggtgcaaa gcgctcgagg agcgcgtccg
     1681 acacgaagag cgcgcgggcg cggctgtcgc gcagcatgaa gtcgtagtcg gcggggctga
     1741 gcagcgtgtt gacagcgatc ggcacgatgc cggctttgat cgcgcccagg aacatcgtcg
     1801 gaaaatcgat gccgtcgagg aggcagagca ggatgcgctg ctcgggcgcg aggccgaggc
     1861 cgcgcaaggc attggcggcg cgatccaccc gctcggccag cacggcatag gtgtaggcgc
     1921 cgcggtcgtc gcggaaagcg gctttgccgc cgcgtcctgc cttcagattg cgctcgatga
     1981 ggtcgacggc ggcattgtag tcgcgcggga tggcgatctc ggggacctcg ctcgccgagc
     2041 catcggcgga catgactcgt ctcctcctgg ttgcagcctt gcgcgcggct gttatcgatc
     2101 gcgacctact aggccgcagc ggcaagttcg ccgtcaagcc tcgtcgtccc ggcgccggcg
     2161 gtccggctat cgcggagcga cggggaggac gggcgccgca tagccgccgc ccgccgccag
     2221 accttgcccg cggggcttca ggcgcagtgc gggctgctcg acgaggtgcc aggagaggaa
     2281 ggcgagcgcc gccgcgatag cgagcgcgag cgggaagagc tgccaccaga gaagccgccc
     2341 gccgctcgca tagagcagcc cctgctgcac cggccagcca tagatgtaga ggccatagga
     2401 aagatcgccg aagcgtgccg cgcgcagcac ggggagccgc cggtcgaggg ccagatacag
     2461 caccagatag gcaccgaaca gcgggaacag cagcaggaag cggccgagcg ggatgctcat
     2521 gagcagcccg gcgagcgcca ggagcgcgat gcgcccgtcg aagatatgcc ggtcgcgaag
     2581 cttgtagagg atcatcccgg caacgaagaa ggcgagcagc cagccgaccg tgccgatgaa
     2641 ataagggctc gccgccgtgt ccagcgagag gcagacgagg cccagcgcca gcagcggcca
     2701 gagcacggaa aggcgcagca gccgcatgct gccgaggacg aggaccagca gatacatgac
     2761 gacttcgcag ggcaggctcc acagcggccc gtcgacgatg ccaccgatgt cgaagccagt
     2821 gaaatgtacg ccgggcaacc cgttgtgctc gacattgagc atgatgttcg agaagacgaa
     2881 gtcgtagagt ccgccgctgg cgaaataggc gtcgagcggc agcgtcgtcg cgagcggccc
     2941 cagcacgacc gcgcagacca ggatgcacag gatgaggccg ggatagatgc ggagcgcgcg
     3001 cttcgccagg aagcggagcg gcgacgcggt cgcctcgaag ctctccgtga cgagaaagcc
     3061 gctgatgatg aagaacacga agacgccgac gacgccgagg
//
LOCUS       DASYNS010000189         2160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1289008, whole genome shotgun sequence.
ACCESSION   DASYNS010000189 DASYNS010000000
VERSION     DASYNS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289008"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VGP50_05855"
     CDS             <1..435
                     /locus_tag="VGP50_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026932.1"
                     /translation="LKEACGVEVEFDVVEWQVLITAARATPDSPSLHGTMALNASSPS
                     SDISVMARYFSSANFAPNGFNFAEWKDDKFDESLKTLAEASDPKTIADAYRAAHERLV
                     DNPPWLFIVHDLNPRAMSTKVKGFVSPQSWFVDLTLVSMSQE"
     gene            444..1856
                     /locus_tag="VGP50_05860"
     CDS             444..1856
                     /locus_tag="VGP50_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV8026933.1"
                     /translation="MAAASAGVSDPCLLGAGELSRAFVARRLSPVDVVDALLARIARL
                     EPKLHAFVDVHAASARLAAEGADKAIRAGHALGPLHGVPVALKDLIDLEGQITAGGSA
                     SQRQRRSTVTATIAKRMIAQGMIVLGKTHTVEFAMGGWGTNQHLGTPWNPWDPATART
                     PGGSSSGSGVAVAARLAPWAIGTDTGGSVRLPASFCGLTGLKVTIGRISTHGIIPLSS
                     TLDTPGPMARSVEDAALLYDVLQGPDPFDATTRGIAAEDPQPMLRRGVRGLRLARLPT
                     GEREGVAPEMLAAYDRAVECLAGLGAEVVDLRLPFRFAELNALQAISQAESYHFNGRL
                     AEDPEAPLDDAVRKRVLAGAKISASEYLGAKRLLREFKQKLYAAMDGVDALLTPTTET
                     AAIPLDEVDQELAPSRFTRFGNLLELCALALPSGFTATGLPLSLQIVCRGYDEAMALR
                     IGHAYQQATDWHSRLPPIGS"
     gene            1940..>2160
                     /locus_tag="VGP50_05865"
     CDS             1940..>2160
                     /locus_tag="VGP50_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8026934.1"
                     /translation="MARFGRSAPLAAALVLWALASASAQGTLRIGMTASDIPLTSGQT
                     DQGGEGMRFMGYTVYDGLINWDLSSATKPS"
BASE COUNT          336 a          759 c          705 g          360 t
ORIGIN      
        1 ctcaaagaag cgtgcggcgt cgaggtcgaa ttcgatgtgg tcgaatggca ggtcctgatc
       61 acggcggcgc gcgcgacgcc cgacagcccg agcctgcatg gcaccatggc gctcaatgcc
      121 agctcgccct cctcggatat cagcgtcatg gcgcgctatt tctcgtcggc caatttcgcg
      181 cccaacggct tcaatttcgc cgagtggaaa gatgacaaat tcgacgagtc cctgaagacg
      241 ctggccgagg cgagcgaccc caaaacgatc gccgacgcct atcgcgccgc gcatgagcgc
      301 ctggtcgaca atccgccctg gctctttatc gtgcatgacc tcaatccgcg ggccatgagc
      361 accaaggtca aaggcttcgt ctcgccccaa tcctggttcg tcgatctgac gctggtcagc
      421 atgtcgcagg agtagagggg gcgatggcgg ccgccagcgc cggcgtcagc gacccctgcc
      481 tgctgggggc aggggagttg tcgcgcgcct ttgtcgcccg ccggctgtcg cctgtcgatg
      541 tggtcgacgc gctgctggcg cggatcgccc ggctcgagcc gaaactgcac gcgttcgtcg
      601 atgtccacgc cgccagtgcc cgcctcgccg cggaaggggc cgacaaggcg atccgcgccg
      661 gccacgcctt gggcccgttg catggcgtgc cggtcgcgct caaagacctg atcgatctcg
      721 aagggcagat tacggccggc ggctcggcga gccagcggca gcgccgctcg accgtcaccg
      781 ccaccatcgc caagcgcatg atcgcgcagg gcatgatcgt gctgggcaag acccatacgg
      841 tggagttcgc gatgggcggc tggggcacca accagcatct gggcacgccg tggaatccct
      901 gggacccggc caccgctcgc acgcccggcg ggtcgagcag cggctcgggc gtggccgtcg
      961 ccgcgcgcct cgcgccctgg gcgatcggca cggacaccgg cggatcggtg cggctgccgg
     1021 cgtcgttctg cggcttgacc gggctcaagg tgacgatcgg ccgcatcagc acccatggca
     1081 tcattccctt gagcagcacg ctcgacacgc ccggccccat ggcgcggtcg gtcgaggacg
     1141 cagctctcct ctatgatgtc ctgcaggggc ccgatccctt tgacgcgacg acgcgcggca
     1201 tcgcggccga ggatccacag ccgatgctcc ggcgcggcgt gcgggggctt cgcctcgccc
     1261 gcctgccgac aggcgagcgc gagggcgtgg caccggagat gcttgccgcc tatgaccgcg
     1321 ccgtcgaatg cctcgccggg ctcggcgccg aggttgtcga tcttcggctg cccttccgct
     1381 ttgccgagct gaacgctctc caggccatca gccaggccga gtcctaccat ttcaacgggc
     1441 gtctggccga agatcctgaa gcgccactcg acgacgccgt gcgcaagcgt gtcctcgccg
     1501 gcgccaagat ctcggccagc gaatatcttg gggccaagcg gctcctgcgc gagttcaagc
     1561 agaagctcta cgctgcgatg gacggtgtcg acgccctcct gacgccgacc accgagacgg
     1621 cggcaatccc gctcgacgag gtcgatcagg aactggcccc gtcccgcttc acgcgcttcg
     1681 gcaacctcct cgagctgtgt gcgctcgctc tgccgagcgg cttcaccgcc accggcctgc
     1741 cgctttcgct gcaaatcgtc tgccgtggct atgacgaggc catggccttg cgcatcgggc
     1801 acgcctacca acaggcgacc gactggcatt cgcgcctgcc gccgatcggc tcctagtcct
     1861 cgcttgcacc agtatgctgg cacacccctt gctttgagcg gatatgagac cgtggccgcc
     1921 tttgaacgcg gaggtgcgga tggctcgctt tggaagatcg gcgccgctcg ccgcggccct
     1981 ggtgttgtgg gcgctcgcct cggcatcggc gcaaggaaca ttgcgcatcg gcatgaccgc
     2041 gtccgacatt cccttgacca gcggccagac cgaccagggc ggcgagggca tgcgcttcat
     2101 gggctacacg gtctatgacg gtctgatcaa ctgggacctt tcgagcgcca ccaagccgtc
//
LOCUS       DASYNS010000190         7650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3474764, whole genome shotgun sequence.
ACCESSION   DASYNS010000190 DASYNS010000000
VERSION     DASYNS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3474764"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1174)
                     /locus_tag="VGP50_05870"
     CDS             complement(143..1174)
                     /locus_tag="VGP50_05870"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEV8026935.1"
                     /translation="MNGAARCVFLYGAYGHTGRFVLDELRRRRWTVLLGGRDAARLRE
                     VAHDGELVVAAEVHEADKLHAALRGSRAILNCAGPFADTAGPLVEAALRARVPYLDIS
                     GEVTVSADLFAKYDLVAKEAGILILPAVGFYGALGHLLVRFAAADWPDAKRVTLAIGL
                     GGWQPTRGTRAVIERMAGRRWVYADGKLQLRTEMKMLEYHFPAPLGRQTVMADYPAPE
                     SVLIPRHLAVPDVDVLMTAVAVRDLRNPVTQAPTRGPDGRSDQRFLLHVQVERAGEIR
                     AAVAQGRDIYATTAPILVGALEEIVTRGPGKAGVMAFSALVEPEQFLAELDRPFLAVA
                     RGSGAPLQP"
     gene            complement(1215..1967)
                     /locus_tag="VGP50_05875"
     CDS             complement(1215..1967)
                     /locus_tag="VGP50_05875"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8026936.1"
                     /translation="MRLENKVALVTGGSTGIGAAIAEALVEAGAAVVVAARREEEGAR
                     KAETLTVRGGRALFVKADVKDMAACKAMVEAAVGAFGALHIAVNSAGIGRAGKLIADE
                     DEGPWQEVIATNLTGAFFSMKHEIPALLASGGGSIINIASIGGLIASAGQSAYQASKH
                     GLLGLTKAAALEYAGRGLRVNAICPGPVRTDMVKRWFAMPGVEEKIVGSIPLRRIAEP
                     REVAMACVYLASEDAAFVTGSSFVMDGGFVVQ"
     gene            complement(1996..2379)
                     /locus_tag="VGP50_05880"
     CDS             complement(1996..2379)
                     /locus_tag="VGP50_05880"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV8026937.1"
                     /translation="MTADAGETVENEIATLATRYVDAWNNHDLDGIMALHAADTTYQM
                     DGQGPVYRGKIEVRATFERQLASVSDLRFELKSLFLGRDHIVFEASIAGIRRGSEPLN
                     LCGIDLITVRDGAIADKHSYAVAAR"
     gene            2489..3085
                     /locus_tag="VGP50_05885"
     CDS             2489..3085
                     /locus_tag="VGP50_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8026938.1"
                     /translation="MARAQKPKQASENVTREKILSTARELFYRDGVRAVGVDTIVAEA
                     DVAKTSLYRWFGSKDDLIASFLANENDAFWDQWDKVASEHEGGPVEALRAHLAWIQGY
                     VVSERFRGCPFLNVTAEFRDAEHPARAVCRENKAELRRRLSDLTRRLTVDDPEILADQ
                     LLLLIDGAFANTQVLGKSGPGRALERGGNTLIDAALKG"
     gene            complement(3159..4010)
                     /locus_tag="VGP50_05890"
     CDS             complement(3159..4010)
                     /locus_tag="VGP50_05890"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEV8026939.1"
                     /translation="MIDASALARTLAQYRGEGYAVLRGVFTAAEVAAIAGAFDRHWAK
                     GMAYPTSFRHGNLLYRLGEDESLGKILRLVQWPSYVDPLLEQMRRDPRWLAILEPLIG
                     RDIKQIINQLHWKPPGAAGAEFAFHQDARFRRPASAYRNLATSYVQTGLAIDAHRRAN
                     GAMRLYPGSQRLGAKELAGAGPVLDQAMGDGQLIAAGLDPQKLVDVELDPGDVALWNV
                     YTIHGSGPNETEGDRRFYLNGYVRAEDCDRGEWAFRDGVPAPLGEPVLVHYEELHTRP
                     EPHFLED"
     gene            complement(4007..5671)
                     /locus_tag="VGP50_05895"
     CDS             complement(4007..5671)
                     /locus_tag="VGP50_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026940.1"
                     /translation="MPAMFRRRPAPVRAALTLVIGAALLCLLFVPARDDENLRFAIDW
                     RPEIEPLLLIGVLALLALAGRRLSPWFRWVLATLVVLAALWQFADALVLSFFDRELNL
                     YLDLPHVPNLLALFYEAAGPWRGFVEVAGVALGAIAAIAVVAYALGAVERALRPHAHA
                     RACLGVVAVALLLVPLPLADGESLLGARTAPTLAHQAALLYRSWAVMHGYDQQYAAAL
                     AAPQPPLGRLDGLKHHDVYIVFFESYGTVVLDDPRYAAKIAPALAEFQATVEQAGYQL
                     LSSRIVSPTFGGGSWLAHGSIESGLKLDQFLARLITQSKRNTLARYMAAAGYRTIEVA
                     PGLKTPAPDSGFWGFAQSYFAGDLGYAGPPFGWFDIPDQYTLRRVDATAAATDGRAPL
                     FAQIVLVSSHTPFVPVPPYVADWSDAGDYRSVTPEAWKRIYAPPDWSALGEPYVASVV
                     YDLQTLGAWLAHRDGDALVIILGDHQPPGFISGEKQPWTVPIHVLSRDPDLIWPFAAA
                     GYAEGAMPPRTGDFKGMESFLDDFLKAFANQEAALAAPATPEHRSD"
     gene            complement(5673..6821)
                     /locus_tag="VGP50_05900"
     CDS             complement(5673..6821)
                     /locus_tag="VGP50_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV8026941.1"
                     /translation="MMPPLYGAASRGDWPLDPAVTYLNHGGYGVAPRAVLATAAEWRE
                     RIERNPMRFMTEELAGAWRSAAAALAAHLGARGEDVVFVANATEGCNAVLRSLDFRSG
                     DEILVTDLAYGAIAKAVRYAAERAGARVVEVEVTLPLADAGAVTMAIAARLGPRTRLV
                     VLDHIASKSALVLPVAEIAGRARAAGARVLIDGAHAPGQVPLDLPATGADWYVGNCHK
                     WLMAPRGCGFLWAPAERQLALHPLAISHGYGSGFIAEFDWTGTRDPCPFLAVPAGIAF
                     HQRLGGPALMARNAALARAASRLLAAEWGTGILGPESAFAAMVTVRLPAGDPTAESAL
                     AVQRRLQKDHNIATAIVVQAGALWVRIAAQAYNELADYERLARALMDS"
     gene            6904..7338
                     /locus_tag="VGP50_05905"
     CDS             6904..7338
                     /locus_tag="VGP50_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV8026942.1"
                     /translation="MAVAAVLQHKGGHVSTIRSIDGVEAAIRKLYDERIGALVVVDRW
                     GKLAGMLSERDVIWALAQHGAEALSFEVHEVMTPDVTTCTPEDRIDDVMHTMTAHRIR
                     HLPVMEEGRLVGLVSMGDLVKQRLEEKKQEAAVLQDIMRVRR"
     gene            7388..>7650
                     /locus_tag="VGP50_05910"
     CDS             7388..>7650
                     /locus_tag="VGP50_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEV8026943.1"
                     /translation="MRVDDFYFELPRHLIAAHPAEPRDAGRLLVVGAALDDRHMLDLP
                     RLLRPGDLLVFNDTRVIPARLLGMRGAAHVEATLHRDLGGGAWR"
BASE COUNT         1318 a         2588 c         2572 g         1172 t
ORIGIN      
        1 gcatcccgag cgcgcggtcg tcggcgtttt cgagctcaag gacaggttcc cgccggtaac
       61 gaggcacgtg atggtggtgc atgacggcag aatggacgac ctgcgcgcgc agcgccggaa
      121 cgcatccgaa tgaggcggcg actcacggtt gcagcggcgc gcctgaaccg cgagccaccg
      181 cgagaaatgg tcggtcgagc tcggcgagaa actgctccgg ttcgaccaac gcggaaaaag
      241 ccatgacgcc cgctttgccc ggcccgcgcg tgacaatctc ctcgagggcg ccgaccaaaa
      301 tcggcgcggt ggtggcgtag atatcgcgac cctgcgcgac cgcggcacga atctcgcccg
      361 cgcgctccac ttgaacatgg agcaggaacc gctgatcgga gcgtccgtcc ggaccgcgtg
      421 tcggcgcctg cgtcacggga ttcctcaggt cgcgaacggc cacggccgtc atcaagacat
      481 ccacatcggg cacggccaga tgacgcggga tgaggacgct ctccggcgcc ggatagtcag
      541 ccatgacggt ctgccgtccg agtggcgctg ggaaatggta ttcgagcatc ttcatctcgg
      601 tgcgaagctg gagcttgccg tcggcgtaga cccaccgtcg cccggccatt cgctcaatta
      661 cggcgcgcgt ccctctggtc ggctgccagc cgcccaggcc aatcgcgagc gtcacacgtt
      721 ttgcatccgg ccagtccgcg gccgcgaacc ggacaaggag atggccgagc gcaccataga
      781 agccgaccgc cggaaggatc aaaattcccg cctccttcgc cacgagatcg tattttgcga
      841 agagatcggc gctcactgtc acctcgccgc tgatatcgag ataagggacc cttgcccgca
      901 aggcagcctc gacgagcggt cccgcggtat cggcgaacgg ccctgcgcaa ttcaagatcg
      961 cacgactgcc gcgcagcgcg gcgtgcagct tgtccgcctc atgcacctct gcggcaacga
     1021 cgagctcacc gtcatgagcg acttcgcgca aacgcgcagc gtcgcggccg ccgagcaaga
     1081 cggtccatcg gcgtcgccgc agctcgtcga gcacgaaacg cccggtgtgc ccgtaggcgc
     1141 cataaagaaa gacgcatcgc gcagcaccgt tcatgcgcct tgctcctctt gtcctaattc
     1201 accgggtcgt cgcgtcactg caccacgaag ccgccatcca tcacgaagga gctgcccgtg
     1261 acgaaagccg cgtcttccga cgccaggtag acgcaggcca tcgccacctc gcggggctct
     1321 gcgatccgcc tgagcgggat cgagccgacg atcttctcct ccacacccgg catcgcgaac
     1381 cagcgcttca ccatgtccgt gcgcacgggc ccggggcaga ttgcgttcac gcgaaggcct
     1441 ctgccggcgt attccagcgc cgccgccttg gtgagaccca agaggccatg cttgctcgcc
     1501 tgataggccg actggcctgc gctcgcgatg agcccgccga tggaggcgat gttgatgatg
     1561 gagccgccgc cgctcgccag gagcgccggg atctcgtgtt tcatcgagaa aaaggctccc
     1621 gtcaggttcg tcgcgatcac ctcctgccat ggcccttcgt cttcatcggc gatgagcttc
     1681 cccgctcggc ctattccggc gctgttgacc gcgatatgga gggccccgaa cgctccgaca
     1741 gcggcctcga ccatggcctt gcaggccgcc atgtccttca catccgcctt gacgaataac
     1801 gctcggccgc cgcgtacggt aagcgtttcc gccttgcgcg cgccctcctc ttcccggcgc
     1861 gcggcgacga ccacggccgc cccggcctcg acgagggctt cggcgatcgc cgcgccaatt
     1921 ccggtgcttc cgccggtaac cagcgcaact ttgttctcca aacgcatgtt caggtctcca
     1981 aattcggttg gaccatcaac gcgccgcgac ggcgtagctg tgtttgtcgg cgatagcgcc
     2041 gtcgcgcacc gtgatgagat cgatgccgca gagattcagc ggctccgagc cgcggcgtat
     2101 gccggcgatc gaggcctcga agacgatgtg gtcgcgcccg agaaagagcg acttgagttc
     2161 gaatctgaga tcgctcaccg acgcaagctg tcgttcgaat gtcgcgcgga cctcgatctt
     2221 gccgcgatag accggaccct gtccgtccat ctgataggtc gtgtcggcgg cgtgcagcgc
     2281 catgatcccg tccagatcat ggttgttcca cgcgtcgacg tagcgcgttg ccagcgtcgc
     2341 gatctcattc tcgactgttt cgcctgcatc ggcagtcacg gcccgctcct tgattgacgc
     2401 tcggtgacgc accattgtgc tacagacctg tctgtttgtg acgatacaga caggtctgta
     2461 gtgcgtcaag gcttttcgga gaatggggat ggcgagagcg cagaagccga agcaggcgag
     2521 cgagaacgtt acgcgcgaga agatcctgtc gacagcgcgc gagctcttct accgggacgg
     2581 ggtgcgggcg gtcggggtgg acaccatcgt ggccgaagcg gacgtggcga aaaccagcct
     2641 ttatcggtgg ttcggctcga aggacgatct catcgcaagc ttcctcgcga acgagaacga
     2701 cgccttctgg gatcaatggg acaaggtggc cagcgagcac gaggggggtc ccgtcgaggc
     2761 gcttcgggcg caccttgcgt ggattcaggg ctatgtcgtc agtgagcgct ttcgcggctg
     2821 tcctttcttg aatgtgactg cggaatttcg cgacgcggag catcccgcgc gcgccgtctg
     2881 ccgagagaac aaggccgaac ttcgacgccg gctgtccgat ctgacgcgtc ggctcaccgt
     2941 cgatgatccg gaaattctcg ccgaccagct gctgcttctg atcgacggcg cgttcgcgaa
     3001 cacccaggtc ttgggcaagt cggggccggg gcgcgccctc gaacgcggcg gcaacaccct
     3061 gatcgacgcc gccctgaagg gatgagcgag cagcggcaag cgagcgtcac cagaacgata
     3121 atcgatgacc agtgcgcagt gccggacttg tcgcggctct aatcttccag gaaatgcggc
     3181 tccggccgcg tgtgcagttc ctcgtaatgc accagcaccg gctcgcccag cggcgcgggc
     3241 acgccgtcgc ggaaggccca ttcgccgcga tcgcaatctt ccgcgcgcac atagccgttg
     3301 agatagaagc ggcggtcgcc ctccgtctca ttggggccgg agccgtgaat cgtgtagacg
     3361 ttccacagcg cgacgtcgcc cggatcgagc tcgacatcga ccagcttctg cggatcgagg
     3421 ccggcggcga tgagctgccc gtcgcccatc gcctggtcga gcaccggacc ggcccccgcg
     3481 agctctttag cacccaggcg ctgcgagccc ggataaagcc gcattgcgcc attggcgcgg
     3541 cgatgcgcgt cgatggcgag gccggtctgc acgtaggacg tcgcgaggtt gcgataggcg
     3601 ctcgccggcc ggcgaaaccg cgcgtcctgg tgaaaggcga attcggcgcc cgcggcgccc
     3661 ggcggcttcc agtggagctg gttgatgatc tgcttgatgt cgcggccgat caggggctcg
     3721 aggatggcga gccagcgcgg atcgcgccgc atctgctcga gcagcggatc gacataggac
     3781 ggccattgca cgaggcgcag gatcttgccg aggctctcat cctcgcccag gcgatagagc
     3841 aaattgccgt gccggaagct cgtcggatac gccatgccct tcgcccaatg gcgatcgaag
     3901 gcgccggcga tcgccgcgac ctcggcggcg gtgaagaccc cgcgcagcac ggcatagcct
     3961 tcgccgcgat actgcgcgag cgtgcgagca agggcggaag cgtcgatcaa tcgctgcggt
     4021 gctccggcgt tgcgggtgcc gcgagcgcgg cttcctgatt ggcgaacgcc ttcaggaaat
     4081 cgtcgagaaa gctttccatt cccttaaaat cgccggtgcg tggcggcatc gcgccctcgg
     4141 catagccggc ggcggcaaag ggccagatga gatcgggatc gcgcgacagc acgtggatcg
     4201 gcacggtcca gggctgtttc tctccggaaa tgaagccggg cggctgatga tcgccgagga
     4261 tgatcacaag cgcatcgccg tcgcgatggg cgagccatgc gcccagcgtc tggagatcgt
     4321 agacgacgct cgcgacatag ggctcaccca gcgccgacca gtccggcggc gcgtagatgc
     4381 gcttccacgc ctcgggcgtg acgctccgat agtcgccggc gtcgctccaa tccgcgacat
     4441 agggcggcac cggcacgaag ggcgtgtggc tcgacaccag cacgatctgc gcgaagagcg
     4501 gggcgcgccc gtccgtcgcc gccgctgtcg cgtcgacgcg gcgcagcgtg tactgatcgg
     4561 ggatgtcgaa ccagccgaag ggcggcccgg catagccgag atcgccggcg aaatagctct
     4621 gcgcgaaacc ccagaagccg ctgtcgggcg ccggcgtctt gaggccgggc gccacctcga
     4681 tggtgcggta gcccgcggcg gccatgtagc gcgccaacgt gttgcgcttg ctctgcgtga
     4741 tcaggcgggc caggaactgg tcgagcttca gcccggactc gatgctgcca tgggcgagcc
     4801 acgagccgcc gccgaaggtc ggcgagacga tgcggctcga cagcagctga tatcccgcct
     4861 gctcgacggt cgcctggaac tcggcgagcg caggcgcgat ctttgccgcg tagcgcggat
     4921 cgtcgagcac gacggtaccg taggattcga agaacacgat atagacgtcg tggtgcttca
     4981 agccgtcgag gcgcccgagc ggcggctgcg gcgcggcgag cgcggccgca tattgttggt
     5041 catagccatg catgacggcc caggaccggt agagcaaggc ggcctggtgc gcgagcgtcg
     5101 gtgccgtgcg cgcgcccagc agcgactcgc catccgccag cggcagcggc accagcaaga
     5161 gcgccaccgc gaccacgccg aggcacgctc tggcatgcgc gtgtggccga agcgcgcgct
     5221 cgacggcacc aagcgcatag gcgaccacgg cgatcgccgc gatggcgccg agcgccacgc
     5281 cggcgacctc gacaaacccg cgccagggtc ctgccgcctc gtagaagagc gccagcagat
     5341 tcggcacatg cggcagatcg agatagagat tgagctcgcg atcgaagaag ctcagcacca
     5401 acgcatccgc gaattgccag agcgcggcca ggacgacgag cgtcgccagc acccagcgga
     5461 accaggggga gagccgccgt cccgctagcg ccaggagggc cagcacgccg atcagcagca
     5521 gcggctcgat ctccggccgc cagtcgatcg cgaagcgcag gttctcgtcg tcccgcgccg
     5581 gcacgaagag aaggcacagc aaagcggcgc cgatgaccag cgtgagcgcg gcgcgcaccg
     5641 gcgcggggcg gcgacgaaac atggcgggca ttctacgaat ccatcagcgc ccttgccagc
     5701 cgctcgtaat cggcgagctc gttataggcc tgcgcggcga ttctcaccca gagcgcgccg
     5761 gcctgcacga cgatcgccgt ggcaatgttg tgatccttct gcagccggcg ctgcaccgcc
     5821 agcgccgact cggcggttgg atcgcccgcg ggcagacgca ccgtcaccat cgcagcgaag
     5881 gcgctctcgg gtccgaggat cccggtgccc cattccgcgg ccaggaggcg tgaagccgcg
     5941 cgcgccaacg cggcgttccg cgccatgagc gccgggccgc ccaggcgctg gtgaaaggcg
     6001 atgcccgccg gcacggcgag gaacgggcag ggatcgcgcg tgccggtcca gtcgaactcg
     6061 gcgatgaagc cgctgccata gccatgggag atggcgagcg gatgaagcgc gagctggcgc
     6121 tcggccggcg cccagaggaa gccgcagccg cgcggcgcca tcagccattt gtggcaattg
     6181 ccgacatacc aatcggcgcc cgtcgcgggc aggtcgagcg gcacctggcc cggcgcatgg
     6241 gcgccgtcga tcagcacccg cgcgccggca gcgcgtgcgc ggccggcgat ctccgccacc
     6301 ggcagaacca gcgcgctctt cgacgcgatg tgatcgagga ccacgagtcg cgtgcgcgga
     6361 ccgagccgcg cggcgattgc catggtgacg gcgccggcat cggcgagcgg cagcgtcacc
     6421 tcgacctcga cgacgcgcgc gcccgcgcgc tcggcggcgt agcgcacggc cttggcgatg
     6481 gcgccatagg cgagatcggt gacgaggatc tcgtcgccgg aacggaagtc gagcgagcgc
     6541 agcacggcgt tgcagccttc cgtcgcgttg gcgacgaaga cgacgtcctc gccgcgcgcg
     6601 ccgagatgcg ccgccagcgc cgccgccgcg ctgcgccagg cgcccgccag ctcttccgtc
     6661 atgaagcgca tgggattgcg ctcgatgcgc tcgcgccatt ccgccgcggt cgccagcacc
     6721 gccctgggcg ccacgccgta gccgccgtga ttgagatagg tgaccgccgg atcgaggggc
     6781 cagtcgccgc gcgacgctgc gccgtagagc ggcggcatca tggcaagcgg tcgctctggc
     6841 agggtggcgg gcacatcccc atgtgtttat cggattcacc ctgtgatcgc caggaggcag
     6901 accatggccg ttgccgcagt tcttcaacac aaaggtgggc atgtctcgac catacgctcg
     6961 atcgacggcg tcgaggcagc gatccgcaag ctctacgatg agcgcatcgg cgcgctggtc
     7021 gtcgtcgacc gctggggcaa gctcgccggc atgctctcgg agcgcgacgt catttgggcg
     7081 ctggcacagc atggcgcgga agcgctgagc ttcgaggtcc acgaggtgat gacacccgac
     7141 gtcacgacct gcacgcccga ggatcgcatc gacgacgtga tgcacaccat gacggcccat
     7201 cgcatccgcc atttgccggt gatggaggaa gggcgcctcg tcggcctcgt cagcatgggc
     7261 gacctcgtca aacagcgcct cgaggagaag aagcaggaag cggccgtgct gcaagacatc
     7321 atgcgggtac gccgctagcc gcgccgcccc ttcccatatc ccctcgctgc cgctacagcg
     7381 acgcgccatg cgcgtcgacg atttctattt cgagctgccg cgacacctca tcgctgccca
     7441 tcccgcggag cccagggatg cagggcggct cctcgtcgtc ggcgcggcac tcgacgaccg
     7501 gcacatgctc gatctgcccc ggctgttgcg gccgggcgac ctcctcgtct tcaacgacac
     7561 gcgggtgatc ccggcgcggc tcctcggcat gcggggcgcg gcgcatgtcg aggcgacgct
     7621 gcaccgcgac ctcggcggcg gcgcctggcg
//
LOCUS       DASYNS010000191         4188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2070140, whole genome shotgun sequence.
ACCESSION   DASYNS010000191 DASYNS010000000
VERSION     DASYNS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2070140"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="VGP50_05915"
     CDS             complement(<1..1712)
                     /locus_tag="VGP50_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026944.1"
                     /translation="MQLPSNPPPLYDASGRPLRLGAELGRGGEGTVYALADRGDLVAK
                     LYHHAPGAEKSAKIVTMARLGNERLLKLAAWPISPIHATPKGGPVLGFLMPKISGHKP
                     AFNLYSPKLRLQEFPSAGWAFLIHAAANAARAFAVIHEAGHVIGDVNHGNLVVAGDAT
                     VKLIDCDSFQVTAKGQQWFCEVGVSTHQPPEFQGLTSYRHLLRTPNHDNFGLAVIVFQ
                     LLFMARHPFSGRYLGAGDMPMERAIREYRFAYGANALALQMKPPPGSLGLAGINPKLA
                     MLFERAFAREGARDGARPKPEEWIAALQELARSLRRCAANAHEHYGALAKCPWCEIEA
                     TGGFVLFPVVFLPGAAGQPGGPGLDIAALWQEMRAIPDPGPPPPLPAPAALARAVTKV
                     SPQVRRARWSLGLRKALAAALALAALVVLPLSKLHGAVFWPWAVATLAVLAWTAIPFL
                     RRKSGRAELRQAVAAAREQWSALERRWAGEAGTDSFAQRRLALEKLKADYDRLPHERL
                     RRLQALETNREGAQLMAFLDRRPLDGAAIKGVGEAKITMLQSYGIETAADIVDERVLA
                     VPGIG"
     gene            complement(1678..2505)
                     /locus_tag="VGP50_05920"
     CDS             complement(1678..2505)
                     /locus_tag="VGP50_05920"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEV8026945.1"
                     /translation="MAGAWRYAYASVTGTSHEKNGSACQDAGSCRVVEAAGGGSVLLA
                     MASDGAGSVQRSAEGAAWAIELFFAEFGAACRERGIAAVDRGLVLSWLRKLRRRIAAR
                     AARAGTRSSEFACTALGAVIADERAVFFQIGDGAIVISSRQDPGGYRWVFWPQHGEFA
                     NTTNFVTQRHASRALEIATHDGPVDEIAIFTDGIERLVLDLSARTAHAPFFRPLFRWL
                     ASAEPVAAGAAAPPIAQYLGSKQVCDRTDDDKTLILATRRPAAAASHAAAVKSAPAL"
     gene            complement(2505..3182)
                     /locus_tag="VGP50_05925"
     CDS             complement(2505..3182)
                     /locus_tag="VGP50_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV8026946.1"
                     /translation="MPNDQLPFPTPDIVGNPEPRCPCLLLLDTSGSMRGQPLAELNAG
                     LMVLKDELTADAMAMQRVEIGIVTFGPVEVKSEFQTPDLWMPPTLKAGGETPMGAAIE
                     QGLKMLEQRKQVYKQTGLSYYRPWIFLITDGGPTDEWKTAASLVRAGDNNEKKAFSFF
                     CVGVEGAKMDILAQISVREPLKLKGLRFRDLFSWLSSSLGAVSRSKPSDAVQLNNPVT
                     PNGWATV"
     gene            3446..3880
                     /locus_tag="VGP50_05930"
     CDS             3446..3880
                     /locus_tag="VGP50_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026947.1"
                     /translation="MDVALRNKATGKLKFHKIGWSWSCFFGSAFVGIPLFRRGLYGWG
                     AVVVALWVIDLLSSDWSLDEELTLSSLEEGLALSAVMFLVGTGLSFFFGAKANTLAGR
                     RYLATGWEFARPNSEEAAAARRAWGVERAFGADSAFGDDDVM"
     gene            complement(3900..>4188)
                     /locus_tag="VGP50_05935"
     CDS             complement(3900..>4188)
                     /locus_tag="VGP50_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026948.1"
                     /translation="EARVYGNLLAALPESCIVSAIHRLHLLDRFDRVVLMEGGEVLDI
                     GTAEELVERAPGFRELWLRYGAPPLSERPIEIAPGAGPWRVLAARSAAPPV"
BASE COUNT          682 a         1379 c         1464 g          663 t
ORIGIN      
        1 ccgatgccgg gcacggcaag cacgcgctca tcgacgatgt cggccgccgt ctcgatgcca
       61 taggattgca gcatcgtgat cttggcctcg ccgacgcctt tgatggcggc gccgtccaac
      121 ggacggcgat cgaggaaggc catcagttgc gcgccctcgc ggttcgtctc cagcgcctgc
      181 aagcggcgca gccgctcatg cggaagccgg tcgtaatcgg ccttgagctt ctcgagtgcg
      241 aggcgccgct gcgcgaagct atcggttccc gcttcgcccg cccagcggcg ctcgagcgcg
      301 ctccattgct cgcgcgccgc cgccaccgcc tgccgaagct ccgctcttcc gcttttccgc
      361 ctgagaaaag ggatggcggt ccacgccagc accgcgagcg ttgcaacggc ccaaggccag
      421 aacaccgcgc cgtggagttt cgagagcggc agcacgacga gcgcggcgag ggcgagagcg
      481 gctgccaagg ccttgcgcag cccgagcgac caccgcgcgc ggcggacctg cggcgacacc
      541 ttcgtcaccg cgcgggcaag cgccgccggt gcggggagcg gcggcggcgg acccggatcg
      601 gggatggccc gcatctcctg ccacagcgcc gcgatgtcga gaccgggtcc gcccggctgc
      661 cccgcggctc ccggcaggaa gacgacgggg aaaagcacga agccgccggt cgcctcgatc
      721 tcgcaccacg ggcatttggc aagcgcgcca taatgctcat gcgcgttggc ggcgcagcgc
      781 cgcaggctcc gcgccagctc ctggagcgcc gcaatccatt cctcgggctt gggacgcgcg
      841 ccgtcgcgcg ctccctcgcg cgcgaacgcc cgctcgaaca gcattgcgag cttgggattg
      901 atcccggcaa ggccgaggct ccccggcggc ggcttcatct gcagcgccag agcattggcg
      961 ccgtaagcga agcgatattc gcggatcgcg cgttccatgg gcatgtcgcc ggcgccgaga
     1021 tagcggccgg aaaacggatg ccgtgccatg aagaggagct ggaacacgat gacggcgagg
     1081 ccgaaattgt cgtggttggg cgtgcgcagc aaatgccgat agctggtaag gccctggaac
     1141 tccggcggct gatgcgtcga gacgccgacc tcgcagaacc attgctggcc cttggcggtc
     1201 acctggaagc tgtcgcaatc gatgagcttg acggtcgcgt cgccggcgac cacgagattg
     1261 ccgtgattga cgtcgccgat gacatggccc gcctcgtgga tcacggcgaa ggcgcgcgcg
     1321 gcattggcgg cggcgtgaat gaggaaggcc cagccggccg agggaaattc ctgaagccgg
     1381 agcttcgggc tgtaaagatt gaaggcgggc ttgtggccgg aaatcttcgg catgaggaag
     1441 ccgaggacgg gcccgccttt gggtgtcgcg tggatcgggc tgatcggcca ggccgcgagc
     1501 ttcaggaggc gctcgttgcc gagcctcgcc atggtgacga tcttcgcgga tttttccgcg
     1561 cccggcgcat ggtgatagag cttggcgacg aggtcgccgc gatcggcgag cgcataaacg
     1621 gtgccttcgc cgccgcggcc gagctcggcg cccagccgca gcggacgccc gcttgcgtca
     1681 tagagcgggg gcggatttga cggcagctgc atgggaagct gcggccgccg gacggcgcgt
     1741 cgccaggatc agggtcttgt cgtcgtccgt gcggtcgcag acctgcttcg atccgagata
     1801 ttgcgcgatc ggcggtgccg ccgcgcccgc ggcgacaggc tccgccgagg cgagccagcg
     1861 gaagagcggg cgaaagaaag gcgcatgcgc cgttcgggcg ctcagatcga ggaccagccg
     1921 ctcgatgccg tcggtgaaga tcgcgatctc gtcgaccggc ccgtcatggg tcgcgatctc
     1981 gagggcgcgc gacgcatggc gctgcgtcac gaagttggtg gtgttggcga actcgccatg
     2041 ctgcggccag aacacccagc gatagccgcc gggatcctgg cgcgacgaga tcacgatggc
     2101 gccgtcgccg atctggaaaa agacggcgcg ctcatcggcg atcacggcgc cgagcgccgt
     2161 gcatgcgaac tcgctcgagc gggtgccggc gcgcgcggcg cgcgcggcaa tccgccggcg
     2221 cagtttccgg agccaggaga ggaccaggcc acgatcgacc gcggcgatgc cgcgctcacg
     2281 acaggccgcg ccgaactcgg cgaagaagag ctcgatcgcc caggccgcgc cttcggcaga
     2341 gcgctggacg ctccccgcgc cgtcgctcgc catcgccaac agcaccgatc cgcctccggc
     2401 agcctcgacg acgcggcagg atccggcgtc ctggcaggcc gagccgttct tctcgtgcga
     2461 tgtgccggtg acggaggcgt aggcatagcg ccacgccccg gccatcacac ggtcgcccag
     2521 ccgttcggcg tcaccgggtt gttgagctgc acggcgtcgc tcggcttcga gcgcgacacg
     2581 gcaccgagcg aactcgacaa ccaggagaag aggtcgcgga aacgcaggcc cttgagcttc
     2641 agcggctcgc gcacgctgat ctgggcgagg atgtccatct tcgcgccctc gacgccgacg
     2701 cagaagaagg agaaggcctt tttctcgttg ttgtcgcccg cgcgcaccag cgacgcggcg
     2761 gtcttccatt cgtcggtcgg accgccatcg gtgatgagga agatccaagg gcggtagtag
     2821 ctcaagccgg tctgcttgta gacctgcttg cgctgctcga gcatcttcaa gccctgctcg
     2881 atcgcggcgc ccattggcgt ctcgccgccc gccttgagcg tcggcggcat ccagagatcg
     2941 ggcgtctgga actcgctttt gacctcgacg ggaccgaagg tgacgatgcc gatctcgacg
     3001 cgctgcatcg ccatggcgtc ggcggtgagc tcgtccttga gcaccatcag gccggcgttg
     3061 agctcggcca agggttgtcc gcgcatcgag cccgaggtgt ccagcagcag caggcaaggg
     3121 cagcgaggtt cggggttccc gacgatatcg ggtgtcggga atggcaattg atcatttggc
     3181 atgacgatct tctcccaagt tccgaggcgg gcgcggactc gacccagatt caggcgtttc
     3241 ttgatgacga aacttttcgg cttatttgcg gtaatcgcaa caacagccgg agtaatgaca
     3301 gccgccgtta gagatacgac tgccgctcct aataatgtgt tgccgggtct cgcgcttcca
     3361 gtacttcgag tcgccggcca aaaaatgcca taagaatcac cctaaagggt tgttgcgcgt
     3421 ccgaacccct cctaaatatg cgtggatgga cgtcgcgctc cgcaacaaag cgaccggtaa
     3481 gctcaagttc cacaagatcg gctggagctg gagctgcttc ttcggatccg cgtttgtcgg
     3541 cattccgctc ttccggcgcg gcctttacgg ctggggcgcg gtcgtcgtcg ccctctgggt
     3601 gatcgatctg ctgtcgtccg attggtcgct cgacgaggag ctgaccttgt cgtcgctcga
     3661 ggaggggctg gccctgtccg ccgtcatgtt tctggtgggc accgggctgt cgttcttctt
     3721 cggcgccaag gccaacaccc tcgccggccg ccgctatctc gcgacgggct gggaattcgc
     3781 gcggcccaac tcggaggaag ccgcggcggc gcggcgcgcc tggggggtcg agcgggcgtt
     3841 cggggccgac tccgcgttcg gcgacgacga cgtgatgtga cggccgcgac ggcggcgcct
     3901 cagaccggcg gcgcggcgct ccgcgcggcg aggacgcgcc aggggccggc gcccggtgcg
     3961 atctcgatcg ggcgctcgct cagcggcggc gcgccatagc gcagccacag ctcgcggaag
     4021 cccggcgcgc gctcgaccaa ctcctcggcg gtgccgatgt cgaggacctc gcctccctcc
     4081 atgagcacga cgcggtcgaa acgatcgagg agatggaggc ggtgaatcgc cgagacgatg
     4141 caggactccg gcaaagcggc gagcagattg ccatagacgc gcgcctcg
//
LOCUS       DASYNS010000192         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1913781, whole genome shotgun sequence.
ACCESSION   DASYNS010000192 DASYNS010000000
VERSION     DASYNS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913781"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VGP50_05940"
     CDS             <1..258
                     /locus_tag="VGP50_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV8026949.1"
                     /translation="MLFDEPTSALDPEMIKEVLDVMVGLAQDGMTMVCVTHEMGFARE
                     VADLMVFMDKGEIVERAPPAQFFATPKSERTRLFLSQILRS"
     gene            complement(298..459)
                     /locus_tag="VGP50_05945"
     CDS             complement(298..459)
                     /locus_tag="VGP50_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HEV8026950.1"
                     /translation="MRKGFASAMLAIFVLAGAASVLGACNTTRGFGEDMSSAGDALSN
                     SAEKTKDKM"
     gene            complement(531..1391)
                     /gene="sseA"
                     /locus_tag="VGP50_05950"
     CDS             complement(531..1391)
                     /gene="sseA"
                     /locus_tag="VGP50_05950"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEV8026951.1"
                     /translation="MPYVNPDALVTTEWLAQHLGAPDLRVVDGSFTLPGVAPTAHALY
                     QQRHIRGAVFFDIDEIADATSSLPHMLPSPEKFALRMRKLGLGDGHKIVVYDSTGLNS
                     AARPWWMLRVFDHRDVAILDGGLPKWLAEGRPVTDDTPAPRERHLTARLNTLLVRDKG
                     QMLDNLTSRREQVVDARSRGRFAGTAEEPRPGLRRGHIPGSVNLPYDELVDPKTRTVL
                     PAEALAARFAASGLERGKPVVASCGSGVTACALVFGLHLLGWPDVAVYDGSWAEWGLP
                     GDTPVATGAG"
     gene            complement(1468..2472)
                     /locus_tag="VGP50_05955"
     CDS             complement(1468..2472)
                     /locus_tag="VGP50_05955"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV8026952.1"
                     /translation="MMIRDGFIETIGNTPLIRLRRASEMTGCTILGKAEFLNPGGSVK
                     DRAALFIVRDAEQRGTLRPGGVIVEGTAGNTGIGLALVGNARGYRTVIVMPETQSQEK
                     KDMLRLCGADLRLVPAVPYSNPMNYVRYSERLAEDIAAREPKGAIWANQFDNLANRDA
                     HVATTGPEIWAQTAGKVDAFVASVGTGGTLAGVAIALKERNPRIRNYLADPLGSAIYY
                     YYAHGELKAEGTSITEGIGQGRITKNLEHAPIDGWFQVTDEEMLPVLFDLVQEEGLIL
                     GGSTGINICGALKVAKELGPGHVIVTVLADYGTRYQSKLFNPAFLRERKLPVPKWLDQ
                     "
BASE COUNT          431 a          884 c          921 g          449 t
ORIGIN      
        1 atgctgttcg acgagcccac ctcggcgctc gatcccgaga tgatcaagga agtgctcgac
       61 gtcatggtgg gtctggcgca ggacggcatg accatggtct gcgtcaccca tgagatgggg
      121 ttcgcccgag aggtcgccga cttgatggta ttcatggaca agggcgagat cgtcgaaaga
      181 gcgccgcccg cccaattctt cgccacgccc aagagcgagc ggacacggct cttcctcagc
      241 cagatcctgc gcagctaagg cgggggagcg gccggagccg ctcctttgcc gacgaaccta
      301 catcttgtct ttcgtcttct ccgccgagtt gctgagcgcg tcgcccgcgg atgacatgtc
      361 ttcgccgaag ccgcgcgtcg tattgcaggc gccgagcacc gacgcggcgc cagccaacac
      421 gaagatcgcc agcatcgccg acgcaaaacc tttacgcatt tatccctcca gctgagaata
      481 cccgtccctg ggaggaacag cggagcgccg gaggcggttc cgcgcttgcc tcaacccgcg
      541 cccgtggcga cgggcgtatc gcccggaagg ccccattcgg cccaggaacc gtcataaacg
      601 gcgacgtcgg gccagcccag gaggtggagg ccgaagacga gcgcgcaagc ggtgacaccg
      661 gagccgcagc tcgccaccac cggcttgccg cgctcgagcc ccgaagcggc gaagcgcgcg
      721 gcgagcgctt cggccggcag caccgtccgg gtcttcggat cgaccagctc gtcatagggg
      781 agattgacgc tgccggggat gtggccgcgc cgcaggcccg gccgcggctc ttccgcggta
      841 ccggcgaagc ggccgcggga gcgcgcgtcg accacctgct cgcggcggct cgtcaggttg
      901 tcgagcatct gccctttgtc gcgcacgaga agcgtgttga gccgcgcggt gagatgccgc
      961 tcgcgcggcg ccggcgtgtc gtccgtcacc ggccgcccct cggccagcca tttcggcagc
     1021 ccgccatcga ggatcgccac gtcgcggtgg tcgaagacgc gcagcatcca ccagggccgc
     1081 gccgccgagt tgagtccggt gctgtcgtaa acgacgatct tgtgaccgtc gcccaagccg
     1141 agcttgcgca tgcggagcgc aaatttctcg gggctcggca gcatgtgggg gagggagctg
     1201 gtcgcgtccg cgatctcgtc gatgtcgaag aacacggcgc cgcgaatatg gcgctgctgg
     1261 tagagcgcgt gcgccgtcgg cgccacgccc ggcagcgtga aagagccgtc gacgacacgg
     1321 agatcgggcg cgcccagatg ctgggcgagc cattcggtcg tgacgagcgc gtcgggattg
     1381 acataaggca tgaacggcgt ctcctccggc cggtcgcggg tcagccggcg aagccgatga
     1441 cgacgcgccg gttctgcttg cccttgttca ttggtcgagc catttcggca cgggcagctt
     1501 gcgctcgcgc aggaaggccg ggttgaagag cttcgactga tagcgcgtgc cgtaatcggc
     1561 gagcacggtg acgatcacat gccccggccc cagctccttt gccactttga gcgcgccaca
     1621 gatgttgatg ccggtcgagc cgccgaggat cagcccctcc tcctgaacga ggtcgaaaag
     1681 caccggcagc atctcctcgt cggtgacctg gaaccagcca tcgatgggcg cgtgttcgag
     1741 gttcttggtg atccggccct ggccgatgcc ttcggtgatg gaggtgccct cggccttgag
     1801 ctcgccatgg gcgtaatagt aatagatggc cgagcccaag gggtcggcca gatagttcct
     1861 gatccgcgga ttgcgctcct tgagcgcgat ggcgacgccg gcgagcgtac cgccggtgcc
     1921 gaccgaggcg acgaaggcgt cgaccttgcc ggccgtctgc gcccagatct cgggcccggt
     1981 ggtggcgaca tgagcgtcgc ggttggcgag attgtcgaat tggttggccc agatcgcgcc
     2041 cttgggctcg cgcgccgcga tgtcctcggc gagccgctcg gagtagcgga catagttcat
     2101 cgggttgctg tagggcaccg ccggcaccag gcggagatcg gcgccgcaga gccgcagcat
     2161 gtcctttttt tcctggctct gcgtctcggg catgacgatg acggtgcgat agccccgggc
     2221 attgccgacg agcgccaggc cgattccggt attcccggcc gtgccctcga cgatgacgcc
     2281 gccggggcgc agcgtgccgc gctgctcagc gtcccggacg atgaagagcg cggcgcggtc
     2341 cttgaccgag ccgccgggat tgaggaactc cgccttgccc aggatggtgc agcccgtcat
     2401 ctccgaggcg cgcctgagcc ggatcagcgg tgtgttgcca atggtctcga tgaagccgtc
     2461 gcgtatcatc atatccaggc cattcttttt cgtggccgca ctatgcggcg acacgggcag
     2521 gggatcaagc cgcggcgccc cacggaggag cgccccgatt ttccgtctcc ccttgcggga
     2581 gagggagggg cccattgcgc agcatcgggt gggtgagggg tgagcttcaa cagacccctc
     2641 accctcccaa cgcttcgcga tgggtccctc cctctcccgc aaggg
//
LOCUS       DASYNS010000193         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1604391, whole genome shotgun sequence.
ACCESSION   DASYNS010000193 DASYNS010000000
VERSION     DASYNS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604391"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..636
                     /locus_tag="VGP50_05960"
     CDS             64..636
                     /locus_tag="VGP50_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV8026953.1"
                     /translation="MPRIEPLSPPYTPEQEARIGAYMRRDAKVPPLAIFRTFARHPAL
                     QDAIKALGRFYLVSEPGRSAGITLRDREIVIDRVCARCRCEYEWGGHVASFAREAGLT
                     PQQVAATARGDAEDPAWPERDRLLIRLVDELHDTATLSDELWRELTAHWSETQLLELL
                     ILAGWYHAISYLANGVHMELEPWAPRFPGS"
     gene            708..1019
                     /locus_tag="VGP50_05965"
     CDS             708..1019
                     /locus_tag="VGP50_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8026954.1"
                     /translation="MAALDLLGRRWSLRVLWELLASPAGFRELQGRCGGMSASVLDTR
                     LKELQQTMIVTLDGERRWALTPLGRELIDALFVLHHWSEKWATQLSSPAKSELRPKRR
                     S"
     gene            1108..>2063
                     /locus_tag="VGP50_05970"
     CDS             1108..>2063
                     /locus_tag="VGP50_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV8026955.1"
                     /translation="MDQSAHFRTTTMRCAPEFGTKGNELGGAERPVGQVVLVLQGGGA
                     LGAYQAGVYQGLSEGGVEPTWIIGTSIGAVNGAIIAGNEPGMRVARLKEFWSRVSDTS
                     LIDAAGISDALGASLSGLRAVVRGVRGLFKPRFDAAAFNVFLPVGIERAAFYSAEPLR
                     ETLRGLVNFDAIDPQRLRLTVGAVNAGSGQMRYFDSRRMKLDVEHVIASSALPPGFAA
                     VKIDGEYFWDGSIYSNTPIEVVFDDNPRRDSLIFAVNVWHADGPPAKSIWQVLAREQD
                     IQFASREDSHIARQEQLHQLRHVVRELAKRIPKEQRNDPEVR"
BASE COUNT          336 a          703 c          674 g          350 t
ORIGIN      
        1 gcttcttgac gtgcttctat tttagaagca tgcttctatt tcagaagcaa cgaggagcgc
       61 gccatgcctc ggatcgaacc gctgtcgcca ccctatacgc cggagcagga ggcgcggatc
      121 ggtgcctata tgcgccggga cgcgaaagtg ccgccgctcg ccatcttccg cactttcgcc
      181 cgccatcccg cgctgcagga cgcgatcaag gccctcggcc ggttctatct cgtgagcgag
      241 cccggccgat ccgccggcat tacgctccgc gatcgcgaga tcgtcatcga ccgcgtctgc
      301 gcccgctgcc gctgcgaata cgaatggggc gggcatgtcg cgtccttcgc gcgagaagcg
      361 ggcctcactc cccagcaggt cgccgcgacg gcccgcggcg atgcggagga tcccgcctgg
      421 ccggagcgcg accgcttgct catccgtctg gtcgacgagt tgcacgacac cgcaaccttg
      481 tcggatgagc tgtggcggga gctgacggcc cattggtccg agacgcagct gctggagctt
      541 ttgatcctcg ccggctggta tcatgcgatt tcctatctcg cgaacggcgt ccacatggag
      601 ctcgagccct gggcaccaag atttcccggc agctgacgcc gaggcggtct gaaacggcgc
      661 cgcggccggg gatcaaggtc cgagggtccg cgaccggccg gccgatcatg gcggctctcg
      721 acctgctcgg ccgccgctgg tcgttgcgcg tcctctggga gctgctggca tcgcccgccg
      781 gctttcgcga gctgcagggc cgctgcggcg gcatgtcggc gagcgtcctc gacacacggc
      841 tcaaagagct gcagcaaacc atgatcgtga cgctcgacgg cgagcgccgc tgggcgctca
      901 cgccgctcgg tcgcgagctg atcgacgctc ttttcgtcct ccatcactgg tcggaaaaat
      961 gggcgacgca gctttcatcg ccggcgaagt ccgagctacg gccgaagcgc cgcagctgac
     1021 ggcggaggac actcgagcga aagactgcag cggcctctat gtctcggcgc tttgatgccg
     1081 tcgcgcggcg cactatatct tggccacatg gaccaatcgg ctcatttccg cacaaccacc
     1141 atgcgctgcg ctcccgaatt cgggaccaaa ggcaacgagc tcggcggcgc cgagcgaccc
     1201 gtcggccagg tcgtgctggt cctgcagggc ggcggcgcgc tcggcgccta tcaagccggc
     1261 gtctaccagg ggctgagcga gggcggcgtc gagcccacct ggatcatcgg cacgtcgatc
     1321 ggagcggtca acggcgcgat catcgccggc aacgagccgg gaatgcgggt cgcgcgcctc
     1381 aaggaattct ggtcgcgcgt cagcgacacc tcgttgatcg atgccgcagg gatctcggac
     1441 gctctcggcg cctccctcag cggcttgcgc gccgtcgtcc ggggcgtccg cggcctcttc
     1501 aagccgcgct tcgacgccgc cgccttcaat gtcttcctgc cggtcggcat cgagcgggcg
     1561 gcgttctatt cggccgaacc gctgcgcgag acactgcgcg gcctggtgaa tttcgatgcc
     1621 atcgatccgc agcgcctgcg tctcaccgtc ggagcagtga acgcgggcag cgggcagatg
     1681 cggtatttcg acagccggcg catgaagctc gatgtcgagc atgtcatcgc ctcgagcgcg
     1741 ctgccgccgg gctttgccgc cgtcaagatc gatggcgagt atttctggga cggcagcatc
     1801 tactcgaaca cgccgatcga ggtggtgttc gacgacaatc cgcgccgcga ctcgctgatc
     1861 ttcgccgtca acgtgtggca cgccgatgga ccgccagcca aatccatctg gcaggtgctt
     1921 gcccgcgagc aggatatcca gttcgcgagc cgcgaggaca gccacatcgc ccgccaggag
     1981 cagctccacc agctccgcca cgtcgtgcgc gagctggcca agcgcattcc gaaggagcaa
     2041 aggaacgatc ccgaggtgag aga
//
LOCUS       DASYNS010000194         4390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_666551, whole genome shotgun sequence.
ACCESSION   DASYNS010000194 DASYNS010000000
VERSION     DASYNS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666551"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VGP50_05975"
     CDS             complement(<1..984)
                     /locus_tag="VGP50_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEV8026956.1"
                     /translation="MRERAAAMAPSARGAERYRRATLTFLTALAVKGVALLTMFFSVK
                     LGLSYLGSERFGLWMTALSAAALLSFATLGLDKGLLNALAAADGSRNRAAARRLVSTT
                     FFLFLAIVAALLLLLAPAYPLVPWARLLNAGAGAAEAAPMMAVLIVCTLLALLGSLVD
                     TVQSAYQEGFINGAWEAAGKLLGLGALAAAIAFGAGLPALALAFAGAPLLAALGNALL
                     LFGRRRPWLAPRLRLVQRSVARRLLGAGSLFFLSQLALSVAYYVDNLIVASLAGSEAV
                     AAYAVTARLFDFPGMLLLLIGGALWPPLAEALAQGDISWAERGLGRLILASL"
     gene            complement(992..1753)
                     /locus_tag="VGP50_05980"
     CDS             complement(992..1753)
                     /locus_tag="VGP50_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV8026957.1"
                     /translation="MIARLFTRGRPEEGIAPEVPAGSRVYAIGDVHGRADLLGHLHQL
                     IHEDAYRRQAPRNVVVYLGDYIDRGQGSAAVIDLLLDDPLPGFERVHLKGNHEDSLLR
                     FLGDVTIGPAWFSYGGDATLTGYGVALPAPGARPQELLHVQRELTRLLPERHRRFFEA
                     LTLAHEEGDYLFVHAGLRPGRPLAAQNPTDLLWIRDEFLSSSEDFGKIVVHGHSITAR
                     PDVQKNRIGIDTGAFASDILTCLVLEGSDWSFIQT"
     gene            2029..3486
                     /gene="rfaE1"
                     /locus_tag="VGP50_05985"
     CDS             2029..3486
                     /gene="rfaE1"
                     /locus_tag="VGP50_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HEV8026958.1"
                     /translation="MAEADLIPLVQALSGARVLCVGDVMLDDYVYGRVERVSPEAPIP
                     VLRVEREQHTLGGAGNVLRNLHALGVEVCFISVTGNDVAGRDISRLIAGLGGCEAHVL
                     TERERTTTLKTRYIAGTQQMLRADREQAAPITGSLREDFLRMVTQVLAHYRVTVLSDY
                     AKGVLADGVASEIIAAARAAGHIVVVDPKGSDYGAYRGATLIKPNRRELGAATAKPVG
                     TAVEVVAAAQALIAQHGFAAVMVSLSQDGMILVEATGETHSFGAEAREVYDVSGAGDT
                     AVAVAAASLGAGATFAQAARLANVAAGLVVGKVGTAVVHGNELIEALVDRDVLATRKV
                     LPLPLALDHVARWRRNGLRIGFTNGCFDLLHPGHVALLEQARGQCDRLVVGLNNDASV
                     ARLKGPGRPVQNEEARAAVLASLASVDLVVLFGEDTPVVLISELKPDLLVKGADYRLD
                     EVVGADIVQAYGGKVVLAALLPGHSTTATIARLAR"
     gene            complement(3497..3946)
                     /locus_tag="VGP50_05990"
     CDS             complement(3497..3946)
                     /locus_tag="VGP50_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEV8026959.1"
                     /translation="MSDARDLSKELARGLCRALAQRGFATLTEVPLATGRRADVLALG
                     REGELVIIEIKSSVADFKSDRKWPEYRQFCDRLYFAVPDGFPRELIPEDCGLIAADPF
                     GGAMLREAPSLPLAAARRRAMLLRFARIAAGRLRRLLDPAIDDPELG"
     gene            4057..>4390
                     /locus_tag="VGP50_05995"
     CDS             4057..>4390
                     /locus_tag="VGP50_05995"
                     /inference="COORDINATES: protein motif:HMM:NF018210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HEV8026960.1"
                     /translation="MTTSKSRVLREAWRIARPYWFSEERWLARCLLAAVLVMNLAQVW
                     INVRINSWRNDFYNTLQSYDETGFLYQVLVFTLLVGASVALFVFGNYLQQLLQIRWRR
                     WMTTVYLRE"
BASE COUNT          718 a         1503 c         1518 g          651 t
ORIGIN      
        1 cagcgacgcc agaatgagcc ggccgaggcc gcgctcggcc cagcttatat cgccttgcgc
       61 caaggcctcg gcgagcggcg gccagagcgc gccgccgatc agcagcaaca gcatgccggg
      121 aaaatcgaag agccgcgccg tcacggcata ggccgcgacg gcttcgctgc ccgcgagcga
      181 cgcgacgatg aggttgtcga cgtaataggc gacgctgagc gcgagctggc tcaggaagaa
      241 gaggctgccg gcgcccagga gccgccgcgc gaccgaccgc tgcacgagcc gaaggcgtgg
      301 cgcgagccag ggccggcggc ggccaaagag cagcagcgcg ttgccaagcg cggccaggag
      361 cggcgctccg gcgaaggcca gagcgagcgc gggaaggccg gcgccgaacg caatggctgc
      421 cgccagcgca ccgagcccga ggagcttccc cgctgcctcc cacgcgccgt tgatgaagcc
      481 ttcctggtaa gcggactgga cagtgtcgac gaggctgccg agcaaggcca ggagcgtgca
      541 gacgatcagc accgccatca tcggcgcggc ctctgccgcg cccgcacccg cgttgaggag
      601 acgcgcccag ggcacgagcg gataggccgg agccaggagc agcagaagcg ccgcgacgat
      661 ggccagaaac aagaagaacg tcgtcgacac gagacggcgc gccgcggcgc ggttgcggct
      721 tccgtcggcg gcggcgagcg cattgagcaa gcccttgtcg agcccgagcg tggcgaagct
      781 caacaacgcc gccgccgaga gcgccgtcat ccacaggccg aagcgctcgc tgccgagata
      841 ggagaggccg agcttgacgc tgaagaacat ggtcaggagc gccacgcctt tgacggcgag
      901 cgccgtcagg aaggtgaggg tggcgcggcg atagcgctcg gcgccgcgtg cgctcggcgc
      961 catcgcggcc gcgcgctccc tcatcgccgt ctcatgtctg gatgaaggac cagtcgcttc
     1021 cttcgagaac gaggcaggtg aggatatcgc tggcgaaggc accggtgtcg atgccgatgc
     1081 ggttcttctg cacgtcgggt cgcgccgtga tgctgtggcc atgcacgacg atcttgccga
     1141 aatcctccga cgaggaaagg aactcgtcgc gaatccacaa aagatccgtc gggttctgcg
     1201 ccgcgagcgg ccggcccggc cgtaaccccg catggacgaa caggtaatcg ccttcctcat
     1261 gggcgagggt cagcgcctcg aagaagcggc gatgccgctc gggcaacagc cgcgtcagct
     1321 cgcgctggac gtgcagcagc tcctgcgggc gtgcccccgg ggcgggcagc gccacgccat
     1381 agcccgtgag cgtggcgtcg ccgccatagg agaaccaggc cggcccgatg gtaacgtcgc
     1441 ccagaaagcg cagcaggctg tcctcgtgat tgcccttgag atggacccgc tcgaagcccg
     1501 gcaacgggtc gtccagcagc agatcgatca ccgccgccga tccctggccc cgatcgatgt
     1561 agtcgccgag ataaacgacg acgttgcgcg gcgcctgccg gcggtaggcg tcctcatgga
     1621 tgagctgatg cagatggccc agcagatccg cccggccgtg gacgtcgccg atggcgtaga
     1681 cgcggctgcc cgccggcact tcgggtgcga taccctcctc cggccggccg cgggtgaaga
     1741 gacgagcgat catgacggcg aatatatggg ggagccgcgg cgcgcgcgcc agcagggccc
     1801 ggcacgccgt cgcaatcact gtctcttgat cgcagttgat tgttcaatgt ccaagtgttc
     1861 cggtacacaa caatccgata cgcgcgggtc aatcgcgagg ccgccccctc ccgccgctcg
     1921 ccccgatcat tcacctcccc gtgatcgcgg gaggccggga ggggggtccg gtccgcccgc
     1981 gcggttttgc ttgccagggc aggctcttcg gccctagaga gacggcccat ggcagaggcc
     2041 gatctcatcc ccctcgttca ggcgttgtcc ggcgcgcgcg tgctttgcgt cggcgacgtc
     2101 atgctcgacg actacgtcta tggccgggtc gagcgtgtct cgcccgaggc gccgatcccg
     2161 gtgctccgtg tcgagcgcga gcagcacacg ctcggcggcg cgggcaacgt gctgcgcaat
     2221 ctccacgcgc tgggcgtcga ggtctgcttc atctccgtca ccggcaacga tgtggccggg
     2281 cgcgacatca gccgcctcat cgccggtctc ggcggctgcg aggcgcatgt gctgaccgag
     2341 cgcgagcgca cgacgacgct caaaacccgc tatatcgccg gcacccagca gatgctgcgc
     2401 gccgaccgcg agcaggcggc gccgatcacc ggctccttgc gcgaggattt cctgcgcatg
     2461 gtgacgcagg tgctcgcgca ttaccgcgtg accgtccttt cggactacgc caagggcgtg
     2521 ctcgccgacg gcgtcgccag cgagatcatc gccgcggcac gcgccgcggg acacatcgtc
     2581 gtcgtcgatc ccaagggcag cgattacggc gcctatcgcg gagcgacgct catcaagccc
     2641 aaccggcgcg aactcggcgc cgccacggca aagccggtcg ggaccgcggt ggaagtcgtc
     2701 gcggcggcgc aggcgctcat cgcccagcac ggctttgccg ccgtgatggt gtcgctgagc
     2761 caggatggca tgatcctcgt cgaagcgacc ggcgagaccc acagcttcgg cgccgaggcc
     2821 cgtgaggtct atgacgtctc gggcgccggc gacacggcgg tggcggtggc ggcagcgtcg
     2881 ctgggcgccg gcgccacctt tgctcaggcc gcgcgcctcg ccaatgtcgc ggcgggcctc
     2941 gtcgttggca aggtcgggac ggccgtggtg catggcaacg agctgatcga ggcgctggtc
     3001 gaccgcgacg tcctggcgac gcgcaaggtg ctgcctttgc cgctcgcgct cgaccatgtg
     3061 gcgcgctggc gacgcaacgg cctgcgcatc ggcttcacca atggctgctt cgacctgctc
     3121 catccgggtc atgtggcgct gctcgagcag gcacgcggtc aatgcgaccg cctcgtcgtc
     3181 ggcctcaaca acgatgcctc ggtcgcgcgg ctgaaggggc ccgggcggcc ggtgcagaac
     3241 gaggaggcgc gcgccgccgt gctggcgtcg ctcgcctcgg tcgacctcgt cgtgctcttc
     3301 ggcgaggaca cgccggtcgt cctcatcagc gagctcaagc ccgatctcct ggtgaaaggc
     3361 gccgattacc ggctcgacga ggtcgtcggc gccgacatcg tccaggccta tggcggcaag
     3421 gtcgtgctcg ccgcgctgct gcccggccac agcaccaccg ccaccatcgc ccgcctcgcg
     3481 cgctgagact gcgcgcttag ccgagctcgg gatcgtcgat tgccgggtcg agcagccgcc
     3541 gcaaccgtcc ggcggcgatg cgcgcgaagc ggagcagcat ggcgcggcgc cgcgccgcgg
     3601 ccagcggcag cgagggcgct tcgcgcagca tggcgccgcc gaacgggtcc gccgcaatca
     3661 ggccgcaatc ctcggggata agctcgcgcg ggaagccgtc ggggacggcg aagtagagcc
     3721 gatcgcagaa ttggcgatat tccggccatt tgcggtcgct tttgaaatcg gctacggacg
     3781 acttgatctc gatgatgacg agttccccct cccgcccgag cgccagcacg tcggcacgcc
     3841 gtcccgtcgc cagcggcact tccgtgagcg tggcgaagcc gcgctgcgcc agcgcgcggc
     3901 acaggccgcg cgccagctcc ttcgagagat cgcgggcatc cgacatgccg acaccaggac
     3961 ctttctcgcg ctatgcgcaa tgttctgcgc aaccgccggg gaccgatcaa gtcccgccat
     4021 gacggtgcgg actcgccgat tgcggagaac agcaacatga cgacgtcgaa gagccgcgtc
     4081 ctgcgcgagg cgtggcgcat cgcccgccct tattggttct cggaggagcg gtggctggcg
     4141 cgctgcctgc ttgccgcggt gctggtcatg aacctggccc aggtgtggat caacgttcgc
     4201 atcaactcct ggcgcaatga tttctataac acgctgcaga gttacgacga aaccggcttc
     4261 ctctatcagg ttcttgtgtt cacgttgctc gtcggcgcgt ccgtcgcctt gtttgttttc
     4321 ggcaattatc tgcagcagct cctgcagatt cgctggcggc gctggatgac cacggtctat
     4381 ctccgcgaat
//
LOCUS       DASYNS010000195        10374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5190223, whole genome shotgun sequence.
ACCESSION   DASYNS010000195 DASYNS010000000
VERSION     DASYNS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10374
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5190223"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VGP50_06000"
     CDS             <1..575
                     /locus_tag="VGP50_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV8026961.1"
                     /translation="AMLLAMVSGVIAGVLVHAKPLAQPAYMVANAAPAPAAAAGKVPA
                     AAGGQPAAAAAAGAQQANAEEGGVEPIGPLLAAASVDDGKNRARVCNACHTFDKGGPN
                     RIGPNLYDTVGEPIAEGHNGFSFSAALAGHKGEKWTVDDLNRWLAKPSNFAKGTKMTF
                     AGLPNAKDRADVIAYLNSLSDSPKPLTASK"
     gene            590..1378
                     /gene="hisN"
                     /locus_tag="VGP50_06005"
     CDS             590..1378
                     /gene="hisN"
                     /locus_tag="VGP50_06005"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEV8026962.1"
                     /translation="MTEDVIAAHLALAEALADAAGPIIRSYFRRKIAVDDKPDATPVT
                     IADRESEAAMRALIEDRFPEHGIIGEEHGRTRADAEYVWVLDPIDGTKSFISGIPLFG
                     TLIALVHCGRPVIGVIDQPVLRERWVGATGRATMLNGTPVRTRPCPALAQATLFCTAP
                     HAMFTGADAAAFARLSGAVKLLRTGADCYAYALIASGFIDLVVEAGLKPYDYAALVPV
                     IEGAGGVLTDWQGHALDLGSDGRVIAAGDPGLAAAAGARLAGVG"
     gene            1463..3295
                     /locus_tag="VGP50_06010"
     CDS             1463..3295
                     /locus_tag="VGP50_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV8026963.1"
                     /translation="MRVAALALTILLAAGAAATDAADVTKSDGLSLFGDLKYGADFSH
                     FDYVNPDAPKGGTVRYSSIGTFDNLNFFILKGNAAAGLGGLFDTLMFPAGDEPASAYG
                     LVAESVEVPADRSWVQYNLRKEARFQDGSPITPEDVIWTFETLKEKGHPRYRLYYADV
                     MKAEKVGARSVKFSFRTGNNRELPQIVGEMPVLSKAYWSTRDFEKTTLEAPLGSGPYK
                     VESFDVGRTITYRRVADYWGKDLPVNRGRNNFDVIRYDYYRDQTVALEAFKAGNYDIR
                     VENVAKNWATGYDSPAFRDGLFKKEEIPNKVPAGMQGFGFNTRKDLFKDPRVRQALSY
                     LFDFEWTNKNLFYDAYTRTESYFSNSELASSGLPSSDELKILEKYKGQIPDEVYTKAY
                     EPPKTDGSGNLRDNMRQALRLLKEAGWSVKGEKLVNDAGQVFTFEFLLDQPEFERIVL
                     PFVQNLGRIGITASIRKIDPAQYENRMKTFDYDMTVVAFGESLSPGNEQRDFWSSAAA
                     DEPGGQNYLGVKSKAVDDLVDLIINAPDRPSLVTRVHALDRVLLHGYYVIPNWHLSYF
                     RVAAWDKFLRPKISPSYALAVDTWWIDPEREQTVEAKKAQEAKP"
     gene            3335..4429
                     /locus_tag="VGP50_06015"
     CDS             3335..4429
                     /locus_tag="VGP50_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HEV8026964.1"
                     /translation="MLAYAVRRILLMIPTLFAIIFVNFVIVQAAPGGPVDQLIAQLKG
                     TGNAALSNVTGGGGELSRPSTSTSGENQSRAARGLDPEFIKALNKLYGFDKPPAERFL
                     TMIKNYLLFDFGTSFYRDVPVVDLIREKLPVSISLGLWATIISYLVSIPLGIRKAVVD
                     GSNFDLWTSAVIVVGYAIPGFLFAILLIVLFAGGSYLHWFPLRGLVSDDWASFSWPHR
                     IADYFWHIALPVISLIIGSFATLTILTKNSFLEEINKQYVVTARAKGISERRVLYGHV
                     FRNAMLLVIAGFPAAFVAILFTGALLVEVIFSLDGLGYLGFQATVGRDYPVMFGTLYI
                     FTLIGLVLTLVRDLTYMFVDPRIDFATRRA"
     gene            4426..5499
                     /locus_tag="VGP50_06020"
     CDS             4426..5499
                     /locus_tag="VGP50_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8026965.1"
                     /translation="MRAEAVAALGSSDRVLGWRVTPLTRRRLANFRANRRGFWSLWIF
                     LALFVLSLFAEFIANDRPLVVAYAGRLYFPVLTDYPETTFGGEFPTEADYRDPYMRQR
                     IAEHGWAVWPPIPFGDQTINYALAKPAPSPPDGVDWLGTDDQGRDVLARLIYGFRISV
                     LFGLALTILSSIIGVAAGAIQGYYGGLVDLLFQRFSEVWGGLPVLYLLIILSSIVQPN
                     FWWLLGIMLLFSWMSLVDLVRAEFLRARNFDYVRAARALGVANPVIIVRHVLPNAMVA
                     TLTFMPFILNGSITTLTSLDFLGFGLPPGSPSLGELLAQGKNNLQAPWLGFTSFAVLA
                     IMLSLLIFIGEAVRDAFDPRKLK"
     gene            5693..7318
                     /locus_tag="VGP50_06025"
     CDS             5693..7318
                     /locus_tag="VGP50_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8026966.1"
                     /translation="MALLSVRDLHVSFATPGAAVEAVRGVSFDLDKGETLALVGESGS
                     GKSVTALSILQLLPYPVATHSRTSSIVFDGKELVGAPEGVMRDIRGNRIAMVFQEPMT
                     SLNPLHTVEKQLTETLFLHKHMGAATARARAIELLKLVGLAEAESRLGAYPHQLSGGQ
                     RQRVMIAMALANEPDILIADEPTTALDVTIQAQILKLLKHLQARFGMALLLITHDLTI
                     VRKMADRVAVMTKGEIVETGTVAEVFARPRHDYTRHLLAAEPKGKPLPVDPPPPVLIG
                     AEGLKVWFPIRSGLFRSISGYVKAVDGVDFTLRAGQTLGVVGESGSGKTTLGLALLRL
                     LESGGAIRYDGRRLDGIAERQMRPLRRELQIVFQDPYSSLSPRLSVGQIVEEGLKVHR
                     IGRDAAARRSLIRDALSEVGLDPEAVDRYPHEFSGGQRQRVAIARALVLKPRFVVLDE
                     PTSALDMSVQAQVVDLLRELQARHQLAYLFISHDLRVVRALAHEVLVMKDGKVVEHGP
                     AAQLFAAPRHPYTRALMAAAFDLEVVESAQTAQ"
     gene            complement(7328..7717)
                     /locus_tag="VGP50_06030"
     CDS             complement(7328..7717)
                     /locus_tag="VGP50_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEV8026967.1"
                     /translation="MSELKPTQALDLWRTVLVESVRSAGPDLSARQLAIVLTVYLTPP
                     PHTVRGLAALLKISKPAVTRALDRLGMLGLARRRRDESDRRSVLVQRTVKGSVFLSDF
                     AEAVASASRQVEQQRRSNLNSAAAVHY"
     gene            complement(7807..9186)
                     /locus_tag="VGP50_06035"
     CDS             complement(7807..9186)
                     /locus_tag="VGP50_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEV8026968.1"
                     /translation="MLSHLISEGGDAIPLTPLAADDLAARTAALDETGRRWIKSIGFT
                     GDPGKVALVPDASGKLSRVLVGISSGEWLWSLAALPDSLPEGRYRLDPEPGAEAATSM
                     ALGWALGCYAFTRYKNPQRSPSKLVWPKNADRPRVERLAQGVGLARDLINTPAQDMGP
                     GELAEAAEALAGRHGARCRVIVGDALLADNYPMIHAVGRASARAPRLIDIVWGDATAP
                     KLTLVGKGVCFDSGGLDLKTSGSMRLMKKDMGGAATLLGLASAVMEARLLLRLRVLIP
                     AVENFVSANAMRPLDVIRTRKGITVEIGNTDAEGRLVLCDALTEAVAEKPALLIDMAT
                     LTGAARTALGPDLPALFTPDDALATDLLRASVAVNDPLWRLPLWKPYRKMLSSTVADI
                     NNVSESAFAGAITAALYLAEFVGPETPWAHIDTYAWNQSSRPGRPEGGEALGLRALYR
                     LIETRFGKS"
     gene            9313..9966
                     /locus_tag="VGP50_06040"
     CDS             9313..9966
                     /locus_tag="VGP50_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEV8026969.1"
                     /translation="MAEYTIYIGNKNYSSWSLRGWLMLKQAGTPFTEVLIPLRGATTR
                     SEIMRHSPSGRVPALHHGDVTVWDSLAIGEYLAEIFPDGRLWPEDPRARAMARAVSAE
                     MHSGFSALRSHLPMNMRSSFPNRGVTPEAQADINRITAAWRDCRKRFGEGGAFLFGDL
                     TIADAMYAPVVSRFRTYKIELDEEAQRYGEAVWALPALQEWLAAARNEPMIIEDAEF"
     gene            9912..>10374
                     /locus_tag="VGP50_06045"
     CDS             9912..>10374
                     /locus_tag="VGP50_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026970.1"
                     /translation="MARGRAQRADDHRGCRVLGARLSTQSFIDAILALPALGRALLSP
                     DRRQIAWSWYRKAPAAEIYVAPTDGSAPPRRLTETPDDTGLLSWTPDGSALVVVEDQG
                     GSEHGQLFRLALDGRMTPLTEAAPAHFPRGGEIDASGRYLVFAANLDPATGR"
BASE COUNT         1723 a         3589 c         3282 g         1780 t
ORIGIN      
        1 ccgccatgtt gctggccatg gtctccggcg tcatcgccgg cgtcctcgtg cacgcgaagc
       61 cgctggcaca gcccgcctac atggtcgcca acgccgctcc cgcccccgct gccgcagcgg
      121 gcaaggtacc ggcggcagcg ggcgggcagc cggccgctgc ggcagcggcg ggcgcgcagc
      181 aggcaaacgc cgaagaaggc ggcgtcgagc ccatcggccc gctcctcgcg gccgccagcg
      241 tcgacgacgg caagaaccgc gcacgggtct gcaacgcctg ccataccttc gacaagggcg
      301 ggcccaaccg gatcgggccg aacctttacg acacggtggg cgagccgatt gccgagggcc
      361 ataatggctt ctccttctcg gcggcgctcg ccgggcataa aggcgagaaa tggacggtcg
      421 acgacctgaa ccgctggctc gccaaaccgt ccaacttcgc caagggcacg aagatgacct
      481 tcgccggcct gcccaatgcc aaagaccggg cggacgtgat cgcttatctc aattctctga
      541 gcgacagccc gaagccgctg accgcgagca agtgaccgga aggcgcggca tgaccgagga
      601 tgtcatcgcc gcccatctcg cgctcgccga agcgctggcc gacgccgcag gaccgatcat
      661 ccgcagctat ttccgccgca agatcgcggt cgacgacaag cccgacgcca cccccgtcac
      721 catcgccgac cgcgagtccg aggcggcgat gcgggcgctc atcgaagatc gcttccccga
      781 gcacggcatc atcggcgagg agcatggccg cacgcgggcc gatgccgaat atgtgtgggt
      841 gctcgacccg atcgatggca ccaaatcttt catcagcggc attccccttt tcggcacgct
      901 gatcgctctc gtccattgcg gccggcccgt gatcggcgtc atcgaccagc cggtgctgcg
      961 cgagcgctgg gtcggcgcga cgggccgcgc cacgatgctc aacggcacgc cggtacgcac
     1021 gcggccctgc cccgcgctgg cgcaggcaac gctcttctgc acggcgcctc acgccatgtt
     1081 caccggtgcc gatgccgcgg ccttcgcgcg gctgagcggt gccgtgaagc tgctgcgcac
     1141 cggggccgac tgctacgcct atgcactcat cgccagcggc ttcatcgacc tcgtcgtcga
     1201 agccgggctc aagccctatg actacgccgc gctggtgccg gtgatcgaag gcgcgggcgg
     1261 cgtcctgacg gattggcagg gacacgccct cgacctcggc agcgacggtc gcgtaatcgc
     1321 cgcgggcgac cccgggctcg ccgcggcggc gggggcgcgg ctcgcaggcg tcggttgaat
     1381 ggaactgccg ccggatcgtg ctgtcgcggc ggcgcgtttc cactagactg ccgggccaag
     1441 aaacgcctgt cgaggatccg ccatgcgcgt cgccgccctt gctctcacca tcctgctcgc
     1501 cgccggcgcc gctgccacgg atgccgccga cgtcaccaag agcgacggcc tctcgctctt
     1561 cggcgacctg aaatacggcg ccgacttcag ccatttcgac tacgtcaatc ccgacgcgcc
     1621 caagggcggg acggtgcgct actcgtcgat cggcaccttc gacaatttga acttcttcat
     1681 tctcaagggc aacgccgccg cgggcctggg tgggctcttc gacacgctca tgtttcccgc
     1741 gggcgacgag ccggcctcgg cctatgggct cgttgccgag agcgtcgagg ttcccgccga
     1801 ccgcagctgg gtacaataca atctgcgcaa ggaggcgcgc tttcaggacg gcagcccgat
     1861 cacgcccgag gacgtgatct ggaccttcga gactctgaag gagaaaggcc atccgcgcta
     1921 ccgcctctat tatgccgatg tcatgaaggc ggagaaggtc ggcgcgcgca gcgtcaaatt
     1981 cagcttccgc accggcaaca accgcgagct gccgcagatc gtcggcgaga tgccggtgct
     2041 gtcgaaggcc tattggtcga cgcgcgattt cgagaagacg acgctcgagg cgccgctcgg
     2101 cagcggcccc tacaaggtcg agagcttcga tgtcgggcgc accatcacct accgccgcgt
     2161 cgcggattac tggggaaagg atctgccggt caatcgcggg cggaacaact tcgacgtcat
     2221 ccgctatgac tactaccgcg accagacggt ggcgctcgag gccttcaagg cgggaaatta
     2281 cgacatccgc gtcgagaatg tcgccaagaa ctgggccacc ggttatgaca gtccggcgtt
     2341 tcgcgacggc ctcttcaaga aggaggagat cccgaacaag gtgccggccg gcatgcaggg
     2401 cttcggcttc aacacgcgga aagacctttt caaggatccg cgggtgcgtc aagccctgag
     2461 ctatctcttc gatttcgaat ggaccaacaa gaatctgttc tacgacgcct atacgcggac
     2521 cgagagctat ttctcgaact ccgagctcgc ctcgtccggc ttgccgagct cggacgaact
     2581 caagatcctc gaaaaatata agggccagat cccggacgag gtctacacca aggcctacga
     2641 gccgccgaag accgacggct ccggcaatct gcgcgacaac atgcggcaag cgctgcggct
     2701 cctcaaggaa gccggctggt ccgtcaaggg cgagaagctc gtcaacgacg ccggccaggt
     2761 tttcaccttc gagttcctgc tcgaccagcc ggaattcgag cgcatcgtgc tgcccttcgt
     2821 gcagaatctc ggacgcatcg gcatcacggc cagcatccgc aagatcgatc cggcgcagta
     2881 cgagaaccgc atgaagacct tcgattacga catgacggtg gtcgcgttcg gcgaatcgct
     2941 ctcgcccggc aacgagcagc gcgacttctg gagctccgcc gccgccgatg aacccggcgg
     3001 ccagaactat ctgggcgtca agagcaaggc ggtcgacgac ctcgtcgacc tcatcatcaa
     3061 cgcgccggac cggccgagcc tcgtgacgcg cgtccacgcg ctcgaccgcg tgcttctgca
     3121 cggctactac gtcatcccga attggcatct gagctatttc cgcgtcgccg cctgggacaa
     3181 gttcctgcgt ccgaagatct caccctccta cgcgctggcg gtcgacacct ggtggatcga
     3241 tccggagcgc gagcagaccg tcgaggcgaa gaaggcgcag gaggccaagc cgtgaccgcg
     3301 ccccgctacg cgcgatcgcc cgattgaccg agcgatgctc gcttacgccg tccgccgcat
     3361 cctgctgatg atcccgacgc tcttcgcgat catcttcgtc aatttcgtca tcgtccaggc
     3421 ggcgcccggc ggcccggtgg accaactcat cgcccagctc aaagggaccg gcaacgccgc
     3481 actcagcaac gtcaccggcg gcggcggcga gctctcccga ccctcgacct cgacctcggg
     3541 cgagaaccag agccgcgccg cgcgtgggct cgaccccgaa ttcatcaagg cgctcaacaa
     3601 gctctacggt ttcgacaaac cgccggccga gcgcttcctg acgatgatca agaattatct
     3661 gctcttcgat ttcggcacga gcttctaccg ggacgtgccg gtggtggacc tgatcaggga
     3721 aaagctgccg gtgtcgatct ccttgggcct ctgggcgacg atcatcagct atctggtgtc
     3781 gatcccgctg ggcattcgca aggcggtagt ggacggcagc aatttcgacc tgtggaccag
     3841 cgccgtgatc gtcgtcggct atgcgatccc cggcttcctc ttcgccatcc tgctcattgt
     3901 cctgttcgcg ggcggcagct acctgcattg gttcccgctg cggggcctcg tctccgacga
     3961 ttgggccagc ttcagctggc cgcaccggat cgccgactat ttctggcaca tcgcgctgcc
     4021 ggtgatatcg ctgatcatcg gcagcttcgc gaccctcacg atcctgacca agaactcctt
     4081 cctcgaggag atcaacaagc aatatgtcgt gacggcccgc gccaaaggca tctccgagcg
     4141 gcgcgtgctc tacggccatg tcttccgcaa tgccatgctg ctggtcatcg ccggctttcc
     4201 cgccgcgttc gtcgccatcc tcttcaccgg cgcgcttctc gtcgaggtga tcttctcgct
     4261 ggacgggctc ggatatctgg gcttccaggc gacggtcggc cgcgactatc cggtcatgtt
     4321 cggcacgctc tacatcttta ccctcatcgg gctggtgctg acgctggtgc gggatctgac
     4381 ctacatgttc gtcgatccgc gcatcgactt cgcgacgcga cgcgcatgag ggccgaggcc
     4441 gtcgccgcgc tcgggtcgag cgaccgcgta ctcggctggc gcgtcacgcc gctcacgcgc
     4501 cggaggctcg ccaacttccg cgccaaccgc cgcggcttct ggtcgctctg gatctttctc
     4561 gcgctcttcg tcctcagcct ctttgccgaa ttcatcgcca acgaccgacc gcttgtcgtc
     4621 gcctacgcgg gccgtctcta ctttccggtc ctgaccgact atcccgagac caccttcggc
     4681 ggcgagttcc cgaccgaagc cgattatcgc gatccctata tgcgccagcg catcgccgag
     4741 catggctggg cggtctggcc gcccatcccc ttcggcgacc agacgatcaa ctacgccctg
     4801 gcaaagcccg ccccttcgcc gcccgacggg gtcgactggc tcggtaccga cgaccagggg
     4861 cgagacgtac tggcgcggct catctacggc ttccgcatct ccgtgctgtt cggtctcgcg
     4921 ctcaccatct tgagctccat catcggcgtg gcggcgggtg cgatccaagg ctattacggc
     4981 ggcctcgtcg acctcctctt ccagcgcttc agcgaagtct ggggcggtct ccccgtgctt
     5041 tacctcctga tcatcctgtc gagcatcgtg cagccgaatt tctggtggct cctcgggatc
     5101 atgctgctgt tctcctggat gtcgctcgtc gacctcgtgc gcgccgaatt cctgcgtgcg
     5161 cgaaacttcg attatgtgcg ggccgcgcgc gcgctcggcg tcgcgaaccc ggtcatcatc
     5221 gtgcgccatg tcctgcccaa cgccatggtg gcgaccctca ccttcatgcc attcatcctg
     5281 aacggctcga tcacgacgct gacttcgctc gatttcctgg gcttcggact gccgcccggc
     5341 tcgccctcgc tcggcgagct cttggcgcaa ggcaagaaca atcttcaggc accttggctc
     5401 ggcttcacgt ccttcgccgt tcttgcgatc atgctgagcc ttctcatctt catcggcgag
     5461 gccgtgcgcg acgcgttcga tccgagaaag ctgaaatgag ggagttgtca gctcccagct
     5521 gtgagttgtc agcgctggat cgaggacctg cgtccttcga caagctcagg atgaggttct
     5581 ctctttttgc catcggcaat cgatgcagca caccattctt cctcatcctg agcttgtcga
     5641 aggacgcacg gcacatgtgc agatctccgc ggacgacgga caggcactag caatggctct
     5701 cctctccgtt cgcgatctac acgtttcctt cgccacgcct ggcgccgccg tcgaagccgt
     5761 gaggggcgtc tccttcgatc tggacaaagg tgagacgctt gcgctcgtcg gtgaatccgg
     5821 ttctggcaag tccgttacgg cgctctccat cctccaactc ctgccctacc cggtcgcgac
     5881 ccactcccgc acaagcagca tcgtctttga cggcaaagaa ctggtgggcg cgcccgaagg
     5941 ggtgatgcgg gacatccgcg gcaaccgcat cgccatggtg ttccaggagc cgatgacctc
     6001 gctcaacccg ctccacaccg tcgagaagca gctcaccgag acgctcttcc tgcacaagca
     6061 catgggcgcc gccactgccc gcgcgcgggc gatcgaattg ctgaagctgg tcggcctcgc
     6121 cgaggcggaa agccggctcg gcgcctatcc gcatcagctc tcgggcggtc agcgccagcg
     6181 cgtcatgatc gccatggcgc tggccaacga gcccgacatc ctcatcgccg acgagccgac
     6241 aaccgcgctc gacgtcacca tccaggcgca gatcctgaag ctcctgaagc atctccaggc
     6301 gcgcttcggc atggccttgc tgctcatcac ccacgatctc accatcgtgc gcaagatggc
     6361 cgaccgcgtc gccgtcatga ccaagggcga aatcgtcgag accggcacgg tcgccgaggt
     6421 gttcgcccgc ccgcgtcacg actatacgcg ccatctgctg gcggccgagc ccaagggcaa
     6481 gccgctgccc gtcgacccgc cgcccccggt cctcatcggc gccgagggat tgaaagtatg
     6541 gttcccgatc cgcagcggcc tcttccgcag catcagcggc tatgtgaagg cggtcgacgg
     6601 cgtcgacttc acgctgcgcg ccgggcagac gctgggcgtg gtcggcgaga gcggatcggg
     6661 caagacaacc ctcgggctgg cgctccttcg cctgctcgag agcgggggcg ccatccgcta
     6721 tgacgggcgc cgcctcgacg gcattgccga gcggcagatg cggccgctcc gccgcgagct
     6781 gcagatcgtg ttccaggatc cctattcgag cctctcgccg cgcctctccg tcggtcagat
     6841 cgtcgaggag gggctgaagg tgcatcgcat cggccgcgat gccgcggcgc gccggtcgct
     6901 gattcgcgac gcgctgagcg aggtcgggct cgaccccgaa gcggtcgacc gctatccgca
     6961 cgaattctcc ggcgggcagc gccagcgcgt cgccatcgcc cgagcgctcg tcctgaagcc
     7021 gcgtttcgtg gtcctcgacg aaccgacctc ggcgctcgac atgtcggtgc aagcgcaggt
     7081 cgtcgatctc ctgcgcgagc tgcaggcgcg ccatcagctg gcctatctct tcatcagcca
     7141 tgatctgcgc gtcgtgcggg cgctggcaca tgaggtgctg gtgatgaagg acggcaaggt
     7201 ggtggagcac ggccccgcgg cgcagctctt cgcggcgccg cggcatccct atacgcgcgc
     7261 cctcatggcc gctgccttcg atctcgaagt ggtggagagc gcccagacgg cgcaatagcg
     7321 gtcgcggtca gtaatggacc gcggcggcac tgttgagatt cgaacgccgc tgctgctcga
     7381 cctggcggct ggcggaggcc accgcttcgg cgaagtcgct gaggaagacc gagcctttga
     7441 cggtgcgctg caccagcacg ctccgccggt ccgactcgtc ccgccggcgc cgggccaggc
     7501 cgagcatgcc caggcggtcg agcgcacggg tgacggcggg tttcgatatc ttgagaaggg
     7561 ccgcaagacc gcgcaccgtg tgcggcggcg gcgtgagata gacggtcagg acgatggcga
     7621 gctgacgtgc cgagagatcc ggacccgcgc tgcggacgct ctccaccagc acggtgcgcc
     7681 acaggtccag cgcctgcgtc ggcttaagct cggacattcc ccgccccttt acgaccaacc
     7741 gcgcggaaat tgtcggtgct tgcggttgag gaaacgtaaa ggaagcagta agcacctgcc
     7801 gattgctcag cttttgccga agcgggtttc gatcaggcga taaagcgcgc gcagccccag
     7861 cgcctcgccg ccttcgggcc ggcccgggcg gctgctttga ttccaggcgt aggtatcgat
     7921 atgcgcccat ggcgtctccg ggccgacgaa ttcggccagg tagagcgccg cggtgatggc
     7981 gccggcaaag gccgattcgg agacattgtt gatatcggcc accgtgctcg acagcatctt
     8041 gcggtaaggc ttccacagcg gcaggcgcca gagcggatca ttgacggcga cgctggcgcg
     8101 cagcaggtct gtcgccagcg catcgtcggg cgtaaagagc gcgggcagat cgggcccgag
     8161 cgccgtgcgc gcggccccgg tgagggtggc catgtcgatg agcagcgccg gcttctccgc
     8221 gacggcctcg gtcaaggcgt cgcagaggac gaggcggcct tcggcatcgg tgttgccgat
     8281 ctcgacggtg atgcccttgc gcgtgcggat gacgtcgagc ggccgcatgg cattggccga
     8341 gacgaagttc tcgaccgccg gaatcagcac gcgaagccgg agcagcagcc gtgcctccat
     8401 gacggcgctg gcgagaccga gcagcgtcgc ggcgcccccc atgtccttct tcatgagccg
     8461 catggacccc gacgtcttga gatcgaggcc gccgctgtcg aagcagacgc ccttgccgac
     8521 gagcgtgagc ttgggcgccg tcgcatcgcc ccagacgatg tcgatgagcc gcggcgcgcg
     8581 cgcactggcg cggccgaccg cgtggatcat cggataattg tccgccagca acgcatcgcc
     8641 gacgatcacg cgacagcgtg cgccatggcg gcccgccagc gcctcggcgg cctctgccaa
     8701 ctcgcccggc cccatatcct gggccggcgt gttgatgagg tcgcgcgcca agccgacgcc
     8761 ctgcgccagg cgctcgacgc gcgggcgatc ggcattcttc ggccagacca gcttcgatgg
     8821 gctccgttgc gggttcttgt agcgggtgaa ggcgtagcag ccgagggccc agcccagagc
     8881 catggatgtc gctgcttcgg cgcccggttc cggatccagg cggtatctcc cttcggggag
     8941 gctgtcggga agcgcggcca atgaccagag ccactcgccg gaggagatcc cgaccagcac
     9001 gcggctcaac ttgcccgagg cgtcgggcac gagcgcgacc ttgccggggt cgccggtgaa
     9061 gccgatggac ttgatccagc gccggcccgt ctcgtccaag gcggcggtcc gcgcagcgag
     9121 gtcgtcggcc gccagcggtg tcaacggaat cgcgtctccg ccctcggaaa tcagatggga
     9181 cagcacggga tcgatcgcct ttctacccca agagcgggat gttgcatgaa ctgacacact
     9241 gtttcagata tgttgcgcgg cgtagtgctg cccgccagag tggggcccgc aacggcgacg
     9301 gggaagatag cgatggccga gtacaccatc tatatcggca acaagaatta ttcctcctgg
     9361 tcattgcgcg gctggctcat gctgaagcag gcgggcaccc cgttcaccga ggtgctgatc
     9421 ccgctgcgcg gcgcgacgac gcggtcggag atcatgcgcc attcgccctc cggccgggtg
     9481 ccggcgctgc accatggcga tgtcacggtg tgggattccc tagccatcgg cgaatacctg
     9541 gccgagatct ttcccgacgg gcgactttgg ccggaagacc cgagggcacg cgcgatggca
     9601 agagcggtga gcgccgagat gcattccggc ttttccgcgt tgcgcagcca tctgccgatg
     9661 aatatgcgct cctcctttcc caaccggggc gtgacgccgg aagcccaggc cgacatcaat
     9721 cggatcaccg cggcctggcg cgactgccgc aagcgcttcg gcgagggcgg cgccttcctc
     9781 ttcggcgatt tgaccatcgc cgacgccatg tacgcgccgg tcgtcagccg gtttcgcact
     9841 tacaaaatcg agctcgatga agaggcgcag cgctatggcg aagccgtctg ggccctgccc
     9901 gcccttcagg agtggctcgc ggccgcgcgc aacgagccga tgatcatcga ggatgccgag
     9961 ttctaggagc ccgtctgagt acccaatcct tcatcgacgc gatactcgcg ctcccggcgc
    10021 ttggccgagc gctgctgtcg cccgaccgcc gccaaatcgc ctggagctgg tatcgcaagg
    10081 cgccggcggc tgagatctat gtcgcgccga cggacggctc ggcgccgccg cgccgcctga
    10141 ccgagacacc ggacgacacc ggccttcttt cctggacgcc cgacgggtcc gccctggtgg
    10201 tggtcgagga ccagggcggc agcgagcatg gccagctctt ccgcctcgcg ctcgacggac
    10261 ggatgacgcc gctcaccgag gcagcgccgg cgcatttccc ccgaggcggc gagatcgacg
    10321 ccagcggacg ctacctcgtc ttcgccgcca atctcgatcc ggcgaccgga cgga
//
LOCUS       DASYNS010000196         5422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4412542, whole genome shotgun sequence.
ACCESSION   DASYNS010000196 DASYNS010000000
VERSION     DASYNS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4412542"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1587)
                     /gene="paaK"
                     /locus_tag="VGP50_06050"
     CDS             complement(256..1587)
                     /gene="paaK"
                     /locus_tag="VGP50_06050"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HEV8026971.1"
                     /translation="MSTQLPPVAPPERSSLDPIEIASRDEISTLQLARLKATLRRAHD
                     HVARFRDACAESGVGPEDLHDLADLAKFPFTTKEDLRRSYPFGMFAVPREKVLRLHAS
                     SGTTGKPTVVGYTRRDLDTWSALVARSIRAAGGRRGDMVHVAYGYGLFTGGLGAHYGA
                     EALGCTVVPVSGGMTERQVQLIHDLKPDIIMVTPSYMLAIADEFERQGLDPRASSLKI
                     GIHGAEPWTAAMRRAIEERMAMHALDIYGLSEVLGPGVANECIETKDGLHLWEDHFYP
                     EVIDPATGEVLPDGTVGELVLTTLTKEALPLLRYRTRDLTRLLPGTARSMRRIERITG
                     RSDDMLIIRGVNLFPSQIEELILREPALSGHYVLELSRPNALDELKVVAEFRSEAAPN
                     SVAVVDRVAARLRHDIKAMIGITAEVEIAPPGRVERSIGKAKRVIDLRPQR"
     gene            complement(1584..2030)
                     /gene="paaI"
                     /locus_tag="VGP50_06055"
     CDS             complement(1584..2030)
                     /gene="paaI"
                     /locus_tag="VGP50_06055"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEV8026972.1"
                     /translation="MAAEPSADRLADAVGRALYAQDNASRALGMVIEEIGPGHAKMRM
                     IVRDDMINGHDLCHGGLIFTLADSAFAFACNAGNQITLAASAEIHFVSPARKGETLVA
                     VARERASAGRSGIYDVEVSEHASGRLVALFRGRARRMDGKIVAGAP"
     gene            complement(2095..2883)
                     /gene="paaG"
                     /locus_tag="VGP50_06060"
     CDS             complement(2095..2883)
                     /gene="paaG"
                     /locus_tag="VGP50_06060"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HEV8026973.1"
                     /translation="MAWETILFEAADGIGTLTLNRPDRLNSFNTKMHEEVASVLDRAE
                     SDSAIRALLITGAGRGFCAGQDLNPKELASTGSFDAGAAIERYYSPLVRRLRAIKKPV
                     IAAVNGPAAGAGANIAFACDIVIAAHGASFLQAFCRIGLVPDAGGTWFLPRLAGTARA
                     AGMMLLGEAVPATLAAEWGLIWKVVDDDKLVAESRGLARRLADGPTVGLGLIKETLNR
                     SLENGLNAQLDVERDFQRLAAASADFREGVAAFLEKRPAKFTGR"
     gene            3075..3452
                     /locus_tag="VGP50_06065"
     CDS             3075..3452
                     /locus_tag="VGP50_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8026974.1"
                     /translation="MVYSILVVEDEDDVRELVATSLRHRGFSVLSVPNAEVALQILME
                     QVKFDLLFTDIVMPGIIDGFELADRAKRLQPDLKILYATGFAHVSRRSAETLHGKLIQ
                     KPYRPDELATEVRRALESDSARA"
     gene            3640..4917
                     /gene="egtB"
                     /locus_tag="VGP50_06070"
     CDS             3640..4917
                     /gene="egtB"
                     /locus_tag="VGP50_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEV8026975.1"
                     /translation="MRPISQDKLDREPVARRYAALRGHSESLAAPLSAEDQTIQSMPE
                     VSPTKWHLAHTSWFFETFLLVPLLSGYRVFDPAYAYLFNSYYESVGPRYPRPERGLLS
                     RPGVADIARYRRHVDESMACLIATLGAAAWRGAAPLIDLGIAHEEQHQELILMDIKHV
                     LSQNPLQPAYRDAPRRERALAVPLGWTEVAGGLRQIGHAGDGFAFDNEMPRHKVWLEP
                     FRLANRPVTCGEYLAFIADEGYRRPQYWLSDGWALAQQEGWRAPLYWREDAAGWTIFT
                     LAGRRAIDLAEAVCHVSFYEADAFARWAGKRLASEAEWEVAALEAGATADGNFMESGL
                     LHPAPAAPGDGLRQMSGDTWEWTASPYVPYPGFRPAAGAVGEYNGKFMSNQMVLRGGA
                     AVTPAAHLRVTYRNFFPPAARWAFAGIRLAEDA"
     gene            4914..>5422
                     /locus_tag="VGP50_06075"
     CDS             4914..>5422
                     /locus_tag="VGP50_06075"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEV8026976.1"
                     /translation="MRAGGALAAFHDLAPAEESFRAAVLSGLARRAKALPCRFLYDER
                     GSALFEQICELPEYYLTRTETAILAEDAREIAQLAGPHAQLIEFGSGAARKVRLLLEA
                     LEEPDAYVAIDISREPLKRAAEEVAADFPGLPVVAICADYLQPLRLPPLPQRGSGRRI
                     GFFPGSTIG"
BASE COUNT          825 a         1794 c         1868 g          935 t
ORIGIN      
        1 acggagagct cgggctcgac ggcgtgctgc gcgatccgat gctcggagag ctcgaatagc
       61 ccatgctccc gcccggacgc gcgctgccgc cggcgcgcgc gtcggccagc gcaggcgaca
      121 gcacgatgaa cgcccagatg agcgcgaaca gcaacttccg caacctggtc atgattggtt
      181 cctcgggcgc tttgacgaca gccgcatctc ttccgttatt tcgtggcccg acgcggcgtc
      241 tgcaaggctc cgggatcacc gctgcgggcg aaggtcgatc actctcttgg ccttgccgat
      301 ggagcgctcg acgcggccgg gcggcgcgat ctcgacctcg gcggtgatgc cgatcatcgc
      361 cttgatgtcg tgccggagcc gggcggcgac gcggtcgacg accgcgacgc tgttgggtgc
      421 cgcctcggag cggaactctg cgaccacctt cagctcgtca agcgcgttgg gccggctcag
      481 ctccagtaca taatggccgg agagcgcggg ctctctcagg atcagctcct cgatctggct
      541 cgggaacagg ttgacgccgc ggatgatgag catgtcgtcg ctgcggccag tgatgcgctc
      601 gatgcgccgc atcgagcgcg ccgtgcccgg cagcagccgc gtcaggtcgc gcgtgcggta
      661 gcgcagcagc ggcagcgcct ccttggtgag cgtggtcagg accaattcgc cgaccgtgcc
      721 gtcgggcagc acctctccgg tcgcgggatc gatcacctcg ggatagaagt gatcctccca
      781 gagatgaagc ccgtccttgg tctcgatgca ttcattggcg acacccgggc ccagcacttc
      841 ggagagcccg tagatgtcga gggcgtgcat cgccatgcgc tcctcgatcg cgcggcgcat
      901 ggccgctgtc cagggctcgg cgccgtggat gccgatcttg agcgaggacg ccctggggtc
      961 gaggccctgg cgctcgaact cgtcggcgat cgccagcata taggacggcg tcaccatgat
     1021 gatgtcgggc ttcaagtcat ggatgagctg cacctgccgc tcggtcatcc cgcccgacac
     1081 cggcacgacc gtgcagccga gcgcctcggc gccgtaatgc gcgccgaggc cgccggtgaa
     1141 gaggccgtag ccataggcga cgtggaccat gtcgccgcgc cgcccgccgg cggcgcgaat
     1201 cgagcgcgcg accagcgccg accaggtgtc gaggtcgcgg cgcgtatagc cgacgaccgt
     1261 cggcttgccg gtcgtgccgc tcgacgcatg gaggcgcaga accttctcgc gcggcacggc
     1321 gaacatgccg aagggatagg agcggcgcag atcttccttg gtggtgaagg ggaacttcgc
     1381 gagatcggcg aggtcgtgca gatcctcggg tccgacgccg ctctcggcgc aggcgtcgcg
     1441 gaagcgcgcg acatggtcgt gcgcgcggcg cagcgtcgct ttgagccgcg cgagctgcag
     1501 cgtcgagatc tcgtcgcgac tcgcgatctc gatcggatcg agactgctgc gctcgggcgg
     1561 cgcaacaggg gggagctgcg tgctcacggc gcgcccgcga cgatcttgcc gtccatgcgc
     1621 cgcgcccgtc cgcggaagag cgcgacgagc cggcccgagg catgctcgct cacctccacg
     1681 tcatagatgc cgctgcggcc ggcgctcgcc cgctcccgcg cgacggcgac cagcgtctcg
     1741 cctttcctcg ccgggctcac gaaatggatt tcagcgctcg cggccagcgt gatctggttg
     1801 ccggcgttgc aggcgaaggc gaaggcgctg tcggcaagag tgaagatcag accgccatgg
     1861 cagagatcat ggccgttgat catgtcgtcg cgcacgatca tgcgcatttt cgcgtgaccc
     1921 gggccgatct cctcgatcac catgccgagc gcgcgcgacg cgttgtcctg cgcatacagg
     1981 gcgcgtccga cggcgtcggc gagtcgatcg gctgacggct cagcggccat taccgtctct
     2041 cgcctggtgc tttcgcagga tcgacgcgga gaaccgagtc gtacgcacgg cgtctcagcg
     2101 tccggtgaat tttgcggggc gcttctcgag aaaggcggcg acgccttcgc ggaaatccgc
     2161 cgacgccgcg gcgaggcgct ggaagtcgcg ctcgacatcg agctgcgcgt tgaggccgtt
     2221 ctcgagcgag cggttcagcg tctccttgat gaggccgagg ccgaccgtcg gcccgtcggc
     2281 gagtcgccgc gcgaggccgc gcgactcggc gacgagcttg tcgtcgtcca ccaccttcca
     2341 gatgagtccc cattccgccg ccagcgtcgc cggcaccgcc tcgcccagca gcatcatgcc
     2401 ggcggcgcgc gccgtgccgg cgagtcgcgg caggaaccag gtgccgccgg catcgggcac
     2461 gaggccgatg cggcagaagg cctggaggaa actcgcgccg tgcgcggcga tgacgatgtc
     2521 gcaggcgaac gcgatattgg cgccggcgcc cgccgcgggg ccgttgacgg cggcgatcac
     2581 gggtttcttg atcgcgcgca agcggcgcac cagcggcgag tagtagcgct cgatcgcggc
     2641 gccggcatcg aaagagccgg tgctggcgag ctctttcgga ttgaggtcct gcccggcgca
     2701 gaagccgcgc ccggcgccgg tgatgagcag tgcgcggatc gccgaatcgc tttcggcgcg
     2761 atcgagcacg gaagcgactt cctcatgcat cttggtgttg aagctgttga ggcgatccgg
     2821 ccggttgagc gtgagcgtgc cgatcccgtc ggccgcttcg aacaggattg tttcccaggc
     2881 catgcgcgcc tcctcgcttg tggtcaagcg tcctatagcg ggcccccggg cgtcaaggcg
     2941 agcgcagtcc cttgaccgtc gcgactatta gtagcaggca atgagcgttt cacatcgaga
     3001 gatattttac gttattcttt ggaggttatt ttctagccac ccctggtggg gtgtgaggca
     3061 cgcgggagca tgggatggtt tacagcatcc tggtcgtgga ggacgaggac gacgtgcgcg
     3121 agttggttgc cacatcgctc agacaccgcg gcttctccgt gctgtcggtg ccgaatgccg
     3181 aggtggcgct gcagattctg atggagcagg tgaagttcga tcttttgttc accgacatcg
     3241 tcatgcccgg catcatcgac ggcttcgagc tcgccgaccg tgccaagcgc ctgcagccgg
     3301 atctgaagat tctctacgcc accggcttcg ctcatgtgtc gcgccgctcg gccgagacgc
     3361 tgcacggcaa gctcattcag aaaccctatc gccccgacga actcgccacc gaagttcgcc
     3421 gcgctctcga gagcgacagc gctcgcgcct gatcaggcaa gggcgccgct gaagccgggg
     3481 cagcagagcc cttgccgagc acgccgctta tccctatttg ccgcttaagc tgcgccgtcg
     3541 tcagaacacg catggcgtta accatgggtc ccagagcggc aactttgccg atccggggcc
     3601 gttgacgctc gattgtggcg ccgtgcgaca ggggaggata tgaggccgat ctcgcaagac
     3661 aagctcgatc gcgagcctgt cgcccgacgc tatgcagcgt tgcgcgggca cagcgaatcg
     3721 ctggcggcgc cgctgtcggc ggaggaccag acgatccagt ccatgccgga ggtgagtccg
     3781 accaaatggc acctggccca tacgagctgg tttttcgaga cgttccttct cgtaccgctc
     3841 ctttcgggct atcgcgtctt tgatccggcg tacgcgtatc tcttcaactc ctactacgag
     3901 tccgtcggcc cgcgttatcc gcggcctgag cgcggtctgc tttcgcgtcc gggcgtcgcc
     3961 gacatcgcgc gctatcgccg ccatgtggac gagtcgatgg cgtgcctcat tgcgacgctg
     4021 ggcgccgcgg catggcgcgg agcggcaccg ctgatcgatc tcggtatcgc ccatgaggag
     4081 cagcatcagg agctgatcct catggatatc aaacatgttc tgtcgcagaa cccgttgcag
     4141 ccggcctatc gggacgcgcc gcggcgcgag cgggccttgg ccgtgccgct tggctggacg
     4201 gaggtggccg gcgggttgcg ccagatcggc catgccgggg acggcttcgc cttcgacaac
     4261 gagatgccgc gccacaaggt ctggctcgag cctttccgtc tggcgaaccg ccccgtcacg
     4321 tgcggcgagt atctcgcctt catcgccgat gaaggctatc gccggccgca atattggctc
     4381 tcggatggct gggcgctggc gcagcaggaa ggctggcgcg cgccgctcta ttggcgcgag
     4441 gacgcggcgg gctggacgat cttcaccctc gctggccggc gcgccatcga cctcgccgag
     4501 gccgtctgcc atgtgagctt ctacgaagcc gatgccttcg cccgctgggc cggcaagcgt
     4561 ctggcgagcg aagcagaatg ggaggtcgca gccctcgagg cgggtgcgac cgcggatgga
     4621 aacttcatgg agagcggctt gcttcatccc gcgcctgcgg cgccaggcga tggcttgcgt
     4681 cagatgagcg gcgacacctg ggagtggacg gcgagccctt acgtccctta tccggggttt
     4741 cgcccggcgg ccggcgcggt cggcgagtac aacggcaaat tcatgtccaa ccagatggtg
     4801 ctgcgcggcg gcgcggcggt cacgccggcg gcgcatctgc gcgtgaccta tcgcaatttc
     4861 ttcccgccgg cggcgcgttg ggcgttcgcc ggcatcaggc ttgcggagga tgcatgaggg
     4921 caggcggcgc cttggcggca tttcacgatc tcgcacccgc cgaggagagc ttccgcgcgg
     4981 cggtcctgtc gggcctcgcg cgacgggcga aggcgttgcc ttgccgcttc ctctatgacg
     5041 agcgcggctc ggcgctcttc gagcagatct gcgagctgcc ggagtattat ctgacgcgca
     5101 ccgagacggc catcctcgcc gaggacgctc gggaaatcgc acagctcgcc ggaccgcatg
     5161 cgcagctgat cgagttcggc agcggcgccg cgcgcaaggt gcgtctcctc ctcgaggcgc
     5221 tcgaggagcc cgacgcctac gtcgcgatcg acatttcgcg cgagccgttg aagcgcgcgg
     5281 ccgaggaagt ggcggccgac tttcccggtc tgccggtcgt cgcgatttgc gccgactatc
     5341 tgcagccgct gcggctcccg cccttgccgc agcgcggcag cggccgccgc atcggctttt
     5401 tccccggctc cacgatcggc aa
//
LOCUS       DASYNS010000197         8645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2539558, whole genome shotgun sequence.
ACCESSION   DASYNS010000197 DASYNS010000000
VERSION     DASYNS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2539558"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1206
                     /locus_tag="VGP50_06080"
     CDS             172..1206
                     /locus_tag="VGP50_06080"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010973349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEV8026977.1"
                     /translation="MLETTIAGSLPKPFWLAKPKMLWAPWAQEGEALAEAKRDATLLV
                     LKEQEDAGIDIVSDGEQSRQHFVHGFLERLDGIDFARRVKMGIRKDRYTADVPTVVTE
                     LRRKGSVHRTEATLARAHTKHRLKFTLPGPMTIVDTIADEHYGDRVKMAMRFAELLNE
                     EARELEAIGVDVVQFDEPAFNVYMDEVKEWGIEALHRAAQGLRCKTAVHICYGYGIKA
                     NIDWKASLGGEWRQYEEIFPALAKSRIDQISLECANSRVPLNLLALLGSKEVLLGAID
                     VASDTVETPEEVARTIRSAMAFVPAERIHPCTNCGMAPLDRSVANGKLAALAAGAALV
                     RKELGRSGSR"
     gene            1319..1600
                     /locus_tag="VGP50_06085"
     CDS             1319..1600
                     /locus_tag="VGP50_06085"
                     /inference="COORDINATES: protein motif:HMM:NF020265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HEV8026978.1"
                     /translation="MPATRATREDRIELRATKEEKRILAAAAAYERLDLTSYVMRNVL
                     PAARAAVDRAERIVLSGRDSARVMALLENPPKPTPALLAAARRRRTARK"
     gene            1609..2133
                     /locus_tag="VGP50_06090"
     CDS             1609..2133
                     /locus_tag="VGP50_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8026979.1"
                     /translation="MDWREEPIGRHHDRKSFDCGSTELNEYLDRYARQNHESGGAKTF
                     VAVPTGAHERVLGFYSIAPGAIEFARVPAKLTKKLGRYEVPVFRLGRLAVDRSVQGRG
                     LGAELLLAAGERALAVAGEVGGVALAVDAKDERAAGWYERFGALALLDDPLKLILPLG
                     VIAAALTEAKKRQR"
     gene            complement(2170..2877)
                     /locus_tag="VGP50_06095"
     CDS             complement(2170..2877)
                     /locus_tag="VGP50_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8026980.1"
                     /translation="MLAVRDLDLFYGDAQALDSVSVTVASGEIVVIIGANGAGKSSLI
                     RAVFGMEKPKGGSIRFEEREIRGLPSHVICNLGIGQVAEGRQIFPNLTVRENLEMGAV
                     LPRARAGAAAAMNRVLALFPRLAERRRQAAGTLSGGEQQMLAIARCLMGQPRLVMLDE
                     PSLGLAPVVVEDVFRVIRDLNARDGLTIVLVEQNVAQSLALAHRGYVLENGRVVMEGS
                     GAALLADDRVRRAYVGL"
     gene            complement(2881..3624)
                     /locus_tag="VGP50_06100"
     CDS             complement(2881..3624)
                     /locus_tag="VGP50_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8026981.1"
                     /translation="MTAAPLLAVQALARRFRGLQAVDGVSFAVPKGGIVALIGPNGAG
                     KTTTFNLIAGALVPDSGRVVIDGSDITGCRPDQACHAGIARTFQLVKPFAALTVIENV
                     MVGALKNGTDMREARRAAAELLERLGLAGKAETAARHLTLPERKRLETARALATRPKI
                     LLLDEVMSGLRPAEVDAMVATLADLSRATGLAILLTEHVMRAVMALSHHIVVLHHGRK
                     IAEGTPEQVSRDPQVLECYLGRAAAGGGG"
     gene            complement(3621..4616)
                     /locus_tag="VGP50_06105"
     CDS             complement(3621..4616)
                     /locus_tag="VGP50_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8026982.1"
                     /translation="MTIGRRRPVVLLASALLVSAAAALPLLPFVDNYVVSVLIVALFA
                     AYFGQAWNIVTGFAGQLSLGHALYVGLGAYAAGALFFHWGVPPWLGMLAGAAGAALAG
                     AVIAALSFRFRVGGVYFALLTIAFAEFTRILFDHFSWVGATSGLYLPVANRAHDDLLR
                     LRGSPAMFYYVLLALTLGGLALSRHLLSRRIGYYWQAIREDEDAAQALGIDVFRYKVA
                     AMGLSAAMTAVGGALLAFYDNVLFPDTTFAIGRSVEIITAPIIGGLGTLAGPLIGAVA
                     LTALGEVTTRLGSGLAIDGLKQWLFGAALLAVVALQPAGLWPWLRSRLGLGGDRR"
     gene            complement(4613..5500)
                     /locus_tag="VGP50_06110"
     CDS             complement(4613..5500)
                     /locus_tag="VGP50_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8026983.1"
                     /translation="MPMARLRQRAMIYLNIIVAGLLTGAVYGLMALGLSVIFGVIRIV
                     NFAHGEMMVLAMYAAVVLNRWLGLDPLTALPLVTAALFVAGYALQRGFLNRYIARPDH
                     EQFLLLLAVATVLTASLLIGFGPDARGVSLAYAYDSYELGALLLDKVRVLAAAGAVAG
                     AAALWAFFRFSRTGTAIRACADNQTGAQVVGLDVKRLYAVTFGLGAACVGAAGCLLLL
                     LIDVEPYIAADYTLLAFIIVILGGLGSIAGALLGGILVGVSEALAGFVLEPSFKSAFS
                     FALLILVLLVRPQGLLGRR"
     gene            complement(5724..6953)
                     /locus_tag="VGP50_06115"
     CDS             complement(5724..6953)
                     /locus_tag="VGP50_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8026984.1"
                     /translation="MKRRLNRRDFGHGLGATGAVLALGAPRLARGEAAPLRIGSLLPR
                     SGFEAVIGQGCQRSVELAKKMLPALGYSFEVIDADTESKPEVARTQVEKLIRDGVHIL
                     VGAFDSSQTAAIAQVAEQNGMPFVINIGANPAITEQGYKFVFRNFPTSIMLGTHGLEL
                     FNALFAATQTQPKTAVLMHVNDTFGQSVVKGINAIMPKLNLPFTIVETIDYDPQVKDL
                     SIEVAKAKAAKADIHMVVTRGVNDAAMMVREMVKQKYEPMGIISPGSPGMYQTDFFKV
                     LGKFSDYAVTNVPWLDPKQGLARDFETGYHKSYPGEVCDIDAGFTFEGVLICADAYKR
                     AKSTKPEALVEALKATNIKDRVMLGGPITFNEKGQNVNLPSACVQNRDEKPTVVLPAE
                     NAEANPVFPVPGWSKRA"
     gene            7139..8089
                     /gene="pip"
                     /locus_tag="VGP50_06120"
     CDS             7139..8089
                     /gene="pip"
                     /locus_tag="VGP50_06120"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEV8026985.1"
                     /translation="MTRQDLFPPIEPYATGLLRVEAPHVLYWEQSGNPHGTPVAFLHG
                     GPGAGATPTHRRFFDPQHYRIIIFDQRGAGRSTPLGSLADNTTAHLVVDMEALRRHLG
                     IERWVLFGGSWGSTLALAYAEAHPERCLGLVLRGVFLCRRAEVDWFLYGIRTVFPEAW
                     RAFSGFLPESERTDLLEAYHRRLTNPDPAVHMPAARAWSTYEGACSTLLPSPDTVAAF
                     GEDRMALGLARIEAHYFRNNVVAPKDDLVRRIDAIRRLPAIIVQGRYDMVCPIASADE
                     LARAWPEAEYVIVPDAGHSAMEPGIRARLIAATEKLRRIV"
     gene            8164..>8645
                     /locus_tag="VGP50_06125"
     CDS             8164..>8645
                     /locus_tag="VGP50_06125"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEV8026986.1"
                     /translation="MRIGGVGSAALALALLAAPSARADGPDLIAGGIGVYDVLHNNTA
                     AQVRGEYRFGYSLFFLEPLVGALVTSDGSFYGYGGLRADLIIAQHYVIMPVATVGYWS
                     RGGGKDLGAATEFKTGAEFAYRFADDSRLGLAFDHISNAGIGRKNPGTESLLLVYSLP
                     "
BASE COUNT         1505 a         2886 c         2918 g         1336 t
ORIGIN      
        1 aaccgcctct tctcgaacga catcgcgccg gtgcggttgg cgcgcgacat cggactcggc
       61 ctggtcaatc gcatcgagcc gctgaagcgc cgcttcatgc gccaggcgat gggccttgcc
      121 ggcccgctgc cgcggctggt gcgcggcgag gccctgtagc caggaggaca catgctcgag
      181 acgacgatcg ccggcagcct gcccaagccc ttctggctcg ccaagccgaa gatgctgtgg
      241 gcgccctggg cccaggaggg cgaggcgctg gcggaagcca agcgcgacgc gacgctgctc
      301 gtgctcaagg agcaggagga cgccggcatc gacatagtca gcgacggcga gcagtcgcgc
      361 cagcatttcg tccatggctt cctcgagcgg ctcgacggca tcgacttcgc ccgccgcgtc
      421 aagatgggca tccgcaaaga ccgctacacc gccgacgtgc cgaccgtcgt caccgagctc
      481 aggcggaagg gatcggtgca ccgcaccgag gcgaccttgg cgcgcgcgca tacgaagcac
      541 cggctcaaat tcacattgcc cggccccatg accatcgtcg acaccatcgc cgacgagcat
      601 tacggcgacc gcgtcaagat ggcgatgcgc tttgccgagc tcctgaacga ggaggcgcgc
      661 gagctcgagg cgatcggcgt cgacgtcgtc cagttcgacg agcccgcctt caatgtctac
      721 atggacgagg tcaaggagtg gggcatcgag gcgctgcatc gcgcggccca ggggctgcgc
      781 tgcaagacgg cggtccatat ctgctacggc tatggcatca aggccaacat cgattggaaa
      841 gcgagcctcg gcggcgagtg gcggcaatat gaggagatct ttccggcgct cgccaagagc
      901 cgcatcgacc agatctcgct cgaatgcgcc aattcgcgcg tgccgctgaa cctcctcgcg
      961 ctcctcggca gcaaggaggt cctcctcggc gcgatcgacg tggcgagcga cacggtcgag
     1021 acgcccgagg aggtcgctcg caccatccgc agcgccatgg ccttcgtgcc ggcggaacgc
     1081 atccatccct gcacgaattg cggcatggcc ccgctcgacc gcagcgtggc caacggcaag
     1141 ctcgccgccc tcgccgccgg cgccgcgctg gtgcgcaagg aattgggacg atcagggagc
     1201 agatgaagtc tacgcgccag cgccgctgcg tcaggagaga tgcccgggat ggcgacggca
     1261 ttgacttgat cggcgtgtac ggatataatc cgtacgaggt gccaaactgg agactgcgat
     1321 gccggcgacg cgcgcgaccc gtgaggacag gatcgagctg cgggcaacca aggaagagaa
     1381 acggattctg gccgccgccg ccgcttatga aaggctcgac ctgaccagct acgtcatgcg
     1441 aaacgttctg cccgcggcgc gcgcagcggt ggaccgcgcc gaacgcatcg tgctcagcgg
     1501 gcgcgacagc gcgcgcgtaa tggcgttgct ggaaaacccg ccgaaaccaa cacctgccct
     1561 cctcgctgct gcacgacgcc gccgcactgc ccggaagtaa ttccgacggt ggattggcgc
     1621 gaagaaccga tcgggcggca tcatgaccga aagagcttcg attgcggctc gaccgagctc
     1681 aacgagtacc tcgatcgcta cgcgagacag aatcacgagt ccggcggcgc caagaccttt
     1741 gtagctgtgc cgactggagc gcatgagcgc gttttgggct tctattcgat cgcgcccggt
     1801 gcgattgagt ttgctcgcgt cccggcgaaa ctgaccaaga agcttggccg ctatgaagtg
     1861 cccgtctttc ggctcggccg tctcgccgtc gatcgctccg tgcagggccg cgggcttggc
     1921 gccgaattgc tgctagccgc gggcgagcgc gctttggccg tcgcggggga agtcggaggt
     1981 gttgcgctgg ctgtcgacgc gaaggacgag cgcgcagccg gatggtatga gcgattcggg
     2041 gctctggcat tgctcgatga tccgctgaaa ttgatacttc cgctcggcgt catcgccgct
     2101 gccctcacag aggcgaagaa acgtcagagg taatctgccg gacggatacg acgttcgagc
     2161 ggctctctcc taaagcccca catacgcgcg ccgtacgcgg tcgtcggcga ggagtgccgc
     2221 gccgcttcct tccatgacga cgcggccgtt ctcgaggacg tagccgcgat gggcgagcgc
     2281 cagcgattgc gcgacgttct gctcgacgag cacgatggtg agcccgtcgc gcgcgttgag
     2341 atcgcggatc acgcggaaga cgtcctcgac caccaccggt gcgagaccga gcgagggctc
     2401 gtccagcatc acgagacggg gctggcccat gaggcagcgg gcgatggcca gcatctgctg
     2461 ctcgccgccc gacagcgtgc cggcggcctg gcggcggcgc tcggcgaggc gcggaaagag
     2521 cgcgaggacg cggttcatcg ccgccgctgc gccggcgcgc gcccgcggca gcaccgcgcc
     2581 catctccaga ttctcgcgca cggtcaggtt cgggaagatc tgccggcctt cggccacctg
     2641 gccgataccg agattgcaga tgacgtgaga cggcagcccg cggatttcgc gttcctcgaa
     2701 acggatcgag ccgcccttcg gcttctccat gccgaagacg gcgcggatga gcgagctctt
     2761 gccggcgccg ttggccccaa tgatgacaac gatctcgccc gacgcgaccg tcaccgagac
     2821 ggaatcgagc gcctgggcgt cgccatagaa gagatcgaga tcgcgcacgg ccagcacggc
     2881 tcagccaccc ccgccggcag cggcgcggcc gagatagcat tcgagcacct gcggatcgcg
     2941 cgagacctgc tccggcgtgc cttcggcgat cttccgtccg tgatgcagca cgacgatgtg
     3001 atgcgagagc gccatgaccg cgcgcatgac atgctcggtg aggagaatcg ccagacccgt
     3061 cgcgcgcgaa aggtcggcga gcgtggcgac catggcgtcg acctcggccg gacgcaggcc
     3121 cgacatcact tcgtcgagca gcaggatctt gggccgcgtc gccagcgcgc gcgccgtctc
     3181 gaggcgcttg cgctccggca gcgtgaggtg tcgcgccgcc gtctcggcct tgccggcgag
     3241 gccgagccgc tccagcagct cggcagcggc gcggcgcgct tcgcgcatgt ccgtgccgtt
     3301 cttgagcgcg ccgaccatga cattctcgat caccgtcagc gcggcgaagg gcttcaccag
     3361 ctgaaaggtg cgggcgatgc cggcgtgaca ggcctggtcc ggtcggcatc ccgtgatgtc
     3421 gctgccgtcg atgacgacgc ggccgctgtc gggcacgagc gcgccggcga tgaggttgaa
     3481 ggtcgtcgtc ttgcccgcgc cgttcgggcc gatgagcgcg acgatgccgc ccttgggcac
     3541 ggcgaagctc acgccatcga ccgcctggag gccgcggaac cgcctggcga gcgcctgcac
     3601 agcgaggaga ggcgcggcgg tcatcgccga tccccgccaa ggccaaggcg cgagcggagc
     3661 catggccaca atcccgcggg ctgcagcgcc accacggcga gcagcgctgc accgaaaagc
     3721 cactgcttca aaccgtcgat ggcgaggccg ctgccgagcc gggtcgtcac ctcgcccagc
     3781 gccgtcagcg ccaccgcgcc gatcagagga cccgcgagcg tgccgagccc gccgatgatc
     3841 ggcgcggtga tgatctcgac cgaccggccg atggcgaagg tcgtatccgg gaaaagcaca
     3901 ttgtcataga aggcgagaag cgcgccgccg accgcggtca tggccgccga cagccccatc
     3961 gccgccacct tgtagcggaa gacgtcgatg ccgagcgcct gggcggcgtc ctcgtcctcg
     4021 cggatggcct gccagtagta gccaatgcgg cgactcagga gatggcggct caaggcgagg
     4081 ccgcccagcg tcagcgccag cagcacgtaa tagaacatcg ccggcgagcc gcgcaggcgc
     4141 aagagatcgt catgcgcccg attggccacc ggcagataga ggccgctggt ggcgcccacc
     4201 caggaaaaat gatcgaagag gatgcgggtg aattcggcga aagcgatggt gaggagcgcg
     4261 aaatagacgc cgccgacgcg gaagcggaag gatagcgccg cgatcaccgc gcccgcgagc
     4321 gccgcgcccg ccgcgcccgc cagcatgccg agccagggcg gcacgcccca atggaagaag
     4381 agcgcgcccg ccgcataggc gccaaggccg acataaagcg cgtgccccag cgacagctgg
     4441 ccggcgaagc cggtgacgat gttccaggcc tggccgaaat aggcggcgaa gagtgcgacg
     4501 atcaagaccg agacgacata gttgtcgaca aagggcagga gcggcagcgc cgccgcggcc
     4561 gacacgagaa gagccgatgc gaggaggacg acagggcgcc gccggccgat ggtcatcgcc
     4621 gccccagcag gccctgcggc cggaccagca ggacgaggat gaggagtgcg aagctgaagg
     4681 cgcttttgaa agagggctcg aggacgaagc cggcgagcgc ctcggagacg ccgacgagga
     4741 tcccgccgag aagcgctccg gcgatgctgc cgagcccgcc caggatgacg atgatgaagg
     4801 cgagcagcgt gtaatcggcg gcgatatagg gctcgacatc gatgagcagc agcaggaggc
     4861 agccggcggc gccgacgcag gcggcaccaa ggccgaaggt gacggcatag aggcgcttca
     4921 cgtcgaggcc gacgacttgc gcgccggtct ggttgtcggc gcaggcgcgg atcgccgtgc
     4981 ccgtgcggct gaagcggaag aaggcccaga gtgccgccgc ccctgccacc gcgccggcag
     5041 cggcaagcac gcggactttg tcgagaagca gggcgccgag ctcgtaggaa tcataggcgt
     5101 aggcgagcga gacgccgcgc gcatcgggac cgaagccgat cagcaggctc gccgtgagga
     5161 cggtcgcgac cgccagcagc agcaggaact gctcatggtc gggccgcgcg atgtagcggt
     5221 tgagaaagcc gcgctgcagc gcatagccgg cgacaaagag cgccgccgtc accagcggca
     5281 gcgcggtcag cggatcgagc ccgagccagc ggttgaggac gactgcagca tacatcgcca
     5341 gcaccatcat ctcgccatgc gcgaaattga cgatgcggat gacgccgaag atgacggaga
     5401 gcccgagcgc catgagccca taaaccgccc ccgtcagcag ccccgccacg atgatgttga
     5461 gatagatcat cgcgcgctgg cgcaagcgcg ccatcggcat ggcgatcttc tctcgccgca
     5521 atcctcaaat tgcccctctc cacccgctct tgcgggggga gagggtaggg tgagggaggt
     5581 ctcgacacgc gaaggctggc gccgagtgaa ctgagacacc ccctcaccct accctctccc
     5641 ccactccgtg ggggcgaggg agaattaaat tttggccgct tcgagattgc gcgaatgcgc
     5701 tggtcatgcg tggcgacgcc gcactatgcg cgtttcgacc agccgggtac cgggaagacg
     5761 ggattggctt cggcattctc cgccggcagc accaccgtcg gcttttcgtc gcggttctgc
     5821 acgcaggccg agggcagatt gacgttctgg cccttctcgt tgaacgtgat ggggccgccc
     5881 agcatcaccc ggtccttgat attcgtggcc ttgagcgcct cgaccagcgc ctcgggcttc
     5941 gtcgatttcg cccgcttgta ggcatcggca cagatgagaa cgccctcgaa ggtgaagccc
     6001 gcgtcgatgt cgcagacctc gcccgggtag ctcttgtggt agccggtctc gaagtcgcga
     6061 gcgaggccct gcttcgggtc gagccatggc acgttggtga cggcgtagtc cgagaacttg
     6121 cccagcacct tgaagaaatc cgtctgatac atgccggggc tgccggggct gatgatgccc
     6181 atcggctcgt atttctgctt caccatctcg cgcaccatca tggcggcgtc gttgacgccg
     6241 cgcgtcacca ccatgtggat gtcggccttg gccgccttgg ccttcgccac ctcgatcgag
     6301 agatccttga cctgcggatc atagtcgatg gtctcgacga tcgtgaaggg caggttgagc
     6361 ttcggcatga tggcgttgat gcccttcacc accgactggc cgaaggtgtc gttcacatgc
     6421 atcaagaccg cggtcttcgg ctgcgtctgg gtagcggcga agagggcgtt gaagagctcg
     6481 agcccgtgcg tcccgagcat gatgctggtc gggaaattgc ggaagacgaa tttgtagcct
     6541 tgctcggtga tcgccggatt ggcaccgata ttgatgacga agggcatgcc gttctgctcg
     6601 gcgacctggg cgatggcggc ggtctggctc gagtcgaagg cgccgacgag gatgtggaca
     6661 ccgtcgcgga tcagcttctc cacctgggtg cgggcgactt cgggtttcga ttccgtatcg
     6721 gcgtcgatga cctcgaagga atagccgagc gccggcagca tcttcttggc gagttcgacc
     6781 gagcgctggc agccctggcc gatcaccgcc tcgaagcccg agcgcggcag caggctgccg
     6841 atcctgaggg gcgcggcttc gccacgcgcc aggcgaggcg cgcccagcgc aaggacggcg
     6901 cccgtcgcgc cgaggccgtg gccgaaatcg cggcggttga ggcgacgttt caagggacat
     6961 cctcccggta accgaaagac ccgggccgca agcttctggg atcgaacgga ttgctggccg
     7021 ccgagctggc ttccacaata ccgcatcatc gagcccgatc aaggttcaga cggccctcgg
     7081 ttgcctttgc gccgccgcgc cttagactgc acctcgttca gcgaggatcc ccatttcgat
     7141 gacgcgccag gatctctttc cgccgatcga gccttatgcc actggcttgc tcagggtcga
     7201 ggcgccgcat gtgctctact gggagcaatc cgggaatccc catggcacgc ccgtcgcctt
     7261 cctccatggc ggacccggcg ccggcgcgac gcccacccat cgccgcttct tcgacccgca
     7321 gcactatcgc atcatcatct tcgaccagcg cggcgccggc cgctccacgc cgctcggctc
     7381 gctggccgac aacacgacgg cgcatctcgt cgtcgacatg gaagcgctgc gccgccatct
     7441 cggcatcgag cgctgggtcc ttttcggcgg ctcctgggga tcgactcttg ctctcgccta
     7501 tgccgaggcg catcccgagc gctgcctcgg cctcgtgctg cgtggcgtgt ttctctgccg
     7561 gcgcgccgag gtcgactggt tcctctacgg catcaggacc gtcttcccgg aagcctggcg
     7621 ggccttctcc ggcttcctgc cggagagcga gcggaccgac ttgctcgaag cctatcatcg
     7681 ccgtctcacc aatcccgatc ccgccgtgca tatgccggcg gcgcgcgcgt ggagtaccta
     7741 tgagggcgcc tgctccaccc tcctgccgag ccccgacacc gtcgccgcct tcggcgagga
     7801 tcggatggcg ctcggcctcg cccgcatcga ggcgcattac tttcgcaaca atgtcgttgc
     7861 gccgaaggac gacctggtgc gccggatcga tgccatccgc cggctgccgg ccatcatcgt
     7921 ccagggccgc tatgacatgg tctgcccgat cgcaagcgcc gacgaactcg cccgcgcctg
     7981 gcccgaggcg gaatacgtga tcgtgcccga tgccggccat tctgcgatgg aaccgggcat
     8041 ccgcgcgcgg ctcatcgcgg cgaccgagaa gctccggcgc atcgtctaac ctcttcgtgt
     8101 cttgccagcg gagttgggcg ccctaagatg gcgcctgcgg ctttacccgc gagggggatc
     8161 agcgtgcgta tcggcggagt tggatcggcg gcgctcgcgc tcgccctctt ggccgcgcca
     8221 tcggcgcggg cagacggacc ggatctcatc gccggcggga tcggcgtcta tgacgtgctg
     8281 cacaacaaca cggccgcgca ggtccggggc gagtaccgtt tcggctacag cctgttcttc
     8341 ctcgaaccgc tggtgggcgc gctcgtcacc agcgacggca gtttctatgg ctatggcgga
     8401 ttgcgcgccg atctgatcat cgcccagcat tacgtcatca tgccggtggc gacggtcggc
     8461 tattggagcc gcggcggcgg caaggacctg ggcgccgcca ccgaattcaa gaccggcgcc
     8521 gagttcgcct accgcttcgc cgacgactcg cggctcggcc tcgccttcga ccacatctcc
     8581 aatgccggca tcggtcggaa gaatccgggc acggagtcgc tcctcctcgt ctactccttg
     8641 ccgtt
//
LOCUS       DASYNS010000198         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4255275, whole genome shotgun sequence.
ACCESSION   DASYNS010000198 DASYNS010000000
VERSION     DASYNS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4255275"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1139)
                     /locus_tag="VGP50_06130"
     CDS             complement(204..1139)
                     /locus_tag="VGP50_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone"
                     /protein_id="HEV8026987.1"
                     /translation="MAEILPDDLVQPFQIDPFGLRGRLVRLGPLLDEILTRHAYPEPV
                     AVILGEAIALATALAGALKYDGVFTLQTKGDGPIRLVVADVGSDGAVRGYAQYDEAKL
                     ANALAAMPGGAAAIGDSVPRLLGAGYLAFTVDQGEHTERYQGIVELQGATLAACAHHY
                     FRQSEQVEAGIKVAIARLPDRQGVPRWRAGSMMIQRLPSEADASKREAAEDGWRRAMI
                     LMSSSTSDELVDPELAPEELLFRLFHEDGVRAYRRHDLVARCRCSRERVERVLRMLPS
                     EELATMKADGHIVVTCEFCSAAYHLDEAEIHALAT"
     gene            complement(1182..2111)
                     /gene="argF"
                     /locus_tag="VGP50_06135"
     CDS             complement(1182..2111)
                     /gene="argF"
                     /locus_tag="VGP50_06135"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV8026988.1"
                     /translation="MDSLTDPSVMATPRHFLDLDRIDGAELRRILDRGVAYKRGLDHS
                     QPMKGKMLAMIFEKPSTRTRLSFEVAMKQLGGDVVVISSADSQLSRGETVADTARVLS
                     RYVDAIMIRTTQAAKLEELAQHATVPVINGLTDASHPCQLMADVMTLEQCKGPAAGKV
                     VAWSGDGNNVAVSWVHAAVRFDFDLRLACPAELRPPERVLDWARAQGGRVSLATDPAE
                     AARGADCVVTDTWVSMGVEASVNRHNMLAPYRVDERLMALAKPGAIFMHCLPAKRGEE
                     VTAGVIDGPQSVVWDEAENRLHAQKGILAWCLG"
     gene            complement(2099..>2599)
                     /locus_tag="VGP50_06140"
     CDS             complement(2099..>2599)
                     /locus_tag="VGP50_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV8026989.1"
                     /translation="KLFAHEWADAPPDILATAKGLGGGFPVGACLATERAAVGMKAGS
                     HGSTFGGNPLAMAVANAVLDVLLTDGFLANVERTARLLHRRLSAIAAKYPGVVAEIRG
                     QGLLTGFKCAVPNTEMVDRLRAGGLLAVGAGDNAVRLLPPLVIGESHVDQGAAIIEAT
                     ARQWTH"
BASE COUNT          410 a          895 c          899 g          395 t
ORIGIN      
        1 tctccgcatg aatcggttcc cccgctgatg ttgtcatcgg ttctgtcgcg gcggagactg
       61 gcacgcgcca gcggcggatt tcaagcggct tgccgccggt gaggtggatt cgctcgcggt
      121 gcgggcgggc acctcaccct ccctgtcgct cgagcgacgg gtcccctccc tcgccccgcg
      181 tcgcgcggag agggaaagaa ggcttacgtg gccagggcgt ggatctcggc ttcgtcgagg
      241 tgataggcgg cgctgcagaa ctcgcaagtg acgacaatgt gaccgtcggc cttcatcgtc
      301 gccagctctt ccgacggcag catgcgcagc acgcgctcga cccgctcgcg cgagcagcgg
      361 cagcgcgcga cgagatcgtg gcgccgatag gcgcgcacgc catcctcatg gaagagccgg
      421 aagagcagct cttcgggcgc gagctcggga tcgacgagct cgtcggacgt gctggagctc
      481 atgaggatca tcgcgcggcg ccagccatcc tcggccgcct cgcgcttgct tgcgtcggcc
      541 tccgacggca gacgctggat catcatgctc ccggcgcgcc agcgcggcac gccctgacga
      601 tcgggcagcc gcgcgatcgc caccttgatc ccggcctcga cctgctccga ttgacggaaa
      661 tagtgatgcg cgcatgcggc gagcgtggca ccctggagct cgacgatgcc ttgatagcgc
      721 tcggtatgct cgccctgatc gacggtgaag gcgagatagc cggcgcccaa gaggcgcggc
      781 acggaatcgc cgatggcggc tgcgccgccg ggcatcgcag ccagcgcatt cgccagcttc
      841 gcctcgtcat attgggcata gccgcgcacc gcgccgtcgc tgcccacatc cgccaccacc
      901 aggcggatcg gcccgtcgcc cttggtctgc aaggtgaaga cgccgtcgta tttgagcgcg
      961 cccgccagcg cggtggcgag cgcgatcgcc tcgccgagaa tgacggcaac gggctccgga
     1021 taggcgtggc gcgtcaggat ctcgtcgagc agaggaccca gccgaacgag ccggccgcgc
     1081 agcccgaagg ggtcgatctg aaagggctga acgagatcat cgggcaggat ttcggccatc
     1141 gctcaccatg aacattcgaa agccgcgtcg gctcaagcgg atcagcccag gcaccaggcg
     1201 agaatgcctt tctgcgcgtg cagccggttc tcggcttcgt cccacaccac cgattgggga
     1261 ccgtcgatga cgccggccgt cacctcctcg ccgcgcttcg cgggcaggca atgcatgaag
     1321 atggcgccgg gcttggccag cgccatcagc cgctcatcga cgcggtaagg cgccagcatg
     1381 ttgtggcgat tgacgctcgc ttcaaccccc atcgacaccc aggtgtcggt gacgacgcag
     1441 tcggcgccgc gcgcggcctc ggcggggtcg gtggcgaggc tgacgcggcc gccctgggcg
     1501 cgggcccagt cgagcacgcg ctcgggcggg cggagctcgg ccgggcaagc gaggcgcaga
     1561 tcgaaatcga aacgcaccgc cgcatgcacc cagctcaccg cgacgttgtt gccgtcgccg
     1621 ctccaggcga cgaccttgcc cgcggcgggg cccttgcact gctcgagcgt catcacatcg
     1681 gccatgagct ggcagggatg gctggcatcg gtgaggccgt tgatcaccgg caccgtggcg
     1741 tgctgcgcca gctcctcgag tttcgccgcc tgcgtggtgc ggatcatgat ggcgtcgaca
     1801 tagcgcgaca gcacgcgcgc cgtgtccgcg acggtctcgc cgcgcgagag ctggctgtcg
     1861 gcgctcgata tgacgacgac gtcgccgccg agctgcttca tcgccacctc gaaggagagc
     1921 cgcgtccgcg tcgacggctt ctcgaagatc atcgccagca tcttgccctt catgggctgc
     1981 gagtggtcga gaccgcgctt gtaggcgacg ccgcgatcga ggatacgccg cagctccgcc
     2041 ccgtcgatgc ggtcgagatc gaggaagtgc cgcggcgtcg ccatgaccga tgggtccgtc
     2101 agtgagtcca ttgccgcgcg gtcgcctcga tgatggccgc cccctgatcc acatggctct
     2161 cgccgatgac gagcggcggc aagagccgca ccgcgttgtc gcccgcgccc accgcgagca
     2221 agccgcccgc gcgcaagcgg tcgaccatct cggtgttggg cacggcgcat ttgaagccgg
     2281 tgagcagccc ctggccgcgg atttcggcaa ccaccccggg atatttggcg gcgatggcgg
     2341 agagccggcg gtggagcagg cgggccgtgc gctcgacatt ggcaaggaag ccgtccgtca
     2401 gaagcacgtc cagcacggca ttcgccaccg ccatggcgag cggattgccg ccgaaggtcg
     2461 agccatgaga gccggccttc atcccgaccg ccgcgcgctc ggtggcaagg caggcgccga
     2521 ccgggaagcc gccgcccagc cccttggcgg tggcgagaat gtcgggcggc gcgtccgccc
     2581 attcatgggc gaagagctt
//
LOCUS       DASYNS010000199         5761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4255449, whole genome shotgun sequence.
ACCESSION   DASYNS010000199 DASYNS010000000
VERSION     DASYNS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4255449"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VGP50_06145"
     CDS             complement(<1..250)
                     /locus_tag="VGP50_06145"
                     /inference="COORDINATES: protein motif:HMM:NF021197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2397 family protein"
                     /protein_id="HEV8026990.1"
                     /translation="MHAFSNSADLFRHVSAEKAVFYRVIMDVFSAAKRQYRLQLRPDE
                     VLSEARWPDIRPHLDEVNAALAQLTAWGNLESQPDTARV"
     gene            complement(1252..2670)
                     /locus_tag="VGP50_06150"
     CDS             complement(1252..2670)
                     /locus_tag="VGP50_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV8026991.1"
                     /translation="MRAIFAQVDATGLSVLHILLAIGVTVHVLLHKRDAGASIGWIGL
                     AWLSPIIGSVLYVIFGINRVKRRANQLRRPRAARPVAEPPISPAGREDHLAALERAGD
                     RITGQPAERGNAVAVLHNGDEAYPQMIAAIEAAETSVALSTYIFRADHAGGAFIDALV
                     RACRRGVQVRVLIDGIGGGYFLSPAYRRLRRHQVPVFRFMHSPLPWRMPFLNLRSHKK
                     ILVLDGRVAFTGGLNIGSENLVAEGPRHPVRDVHFRVEGPVVAQLVDAFAADWFFAAG
                     ENLVGDAWFPPLAGVGEAVARVVVSGPDEDLEKIEFMILEAIACARQSIKVTTPYFLP
                     DDGLITALALAAMRGVAVDVIVPARGNHLAVDWAMRAQIGPLLAAGCRIWRSPPPFNH
                     SKMMTVDGSWCLIGSANWDVRSFRLNFELTVEVYHADIVQRIDDLMAARRGALMTGTE
                     LDDRPPSVKLIDSGARLMLPYL"
     gene            complement(2853..3044)
                     /locus_tag="VGP50_06155"
     CDS             complement(2853..3044)
                     /locus_tag="VGP50_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8026992.1"
                     /translation="MLAGIDLTRVADIDAADSILNIVARGTDAVADAAERGMEGIVAL
                     LHMSPGLLARGIALRRRSR"
     gene            3661..>5761
                     /locus_tag="VGP50_06160"
     CDS             3661..>5761
                     /locus_tag="VGP50_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8026993.1"
                     /translation="MPESLFEQDLTAYLPGFLVSMEQGAAEMRRATMARAEGAVLLVD
                     IVGFVALADRLADQGRRRAETLSILLDAYFGQITDSILAHGGDVITFAGDGIVATWSR
                     SAHLAENARLAAQCAIELQSSLEPLERRMRLTLRQRVVIGAGEILLCTVGGAVSRRYA
                     LAAGAALDEAVRFYHHAGRGDILLGPTTRQALATSAEIEERDGVAFLVRIGARLSSPP
                     SGEAPQQEIATSHLTPYVAPAVVERVRAGQGAWLGEFRTVTTLFLGVGGVDVAGNFLD
                     GLQNATEIVERELARFDGSLDRVSIDEKGLGYLITFGVPSKAHEDDAIRALRAALALR
                     AALRAAGLAPSIGGATGELFCGRYGGSRRQTYSVVGEAANIAARLRQHVNDDIICDEA
                     TCALARNAIAFADLGTIEVKGKAGAIRIHRPLRELLDKPWRAESELVGRAAERAALRE
                     LIADRASGGPIMIEGEAGIGKSRLLAEIADIAGEHGASVIAAAGAQIEEATPYFPWRR
                     ALRGLFAEAEGEALRAAILATLADDDQLRVLAPLLNDVLPIGIAENELTREIAGGARS
                     SLTQRLVIRLFEKRLGERPLILVFDDAHWFDDASAALLRQLMHAVPALVVVIASRPPA
                     KGASSAFVPHLGDKGERRITLGALERRAIERLVDLKLGVTAAPAPLYDFVFRRAAGHP
                     LYTEELVLGLRAGGSLEI"
BASE COUNT          987 a         1899 c         1885 g          990 t
ORIGIN      
        1 acacccgcgc ggtgtcgggt tgtgattcga gattccccca ggcggtaagt tgggcgaggg
       61 cagcgttgac ttcatcgaga tgaggccgga tgtccggcca gcgagcctct gacaacactt
      121 cgtccggccg caattgcagg cgatattgcc gtttcgcagc agaaaacacg tccatgatga
      181 cacgatagaa gacggctttt tcggcgctaa catgccggaa caggtcggcc gagttgctga
      241 aagcgtgcat tgcgtgctcg tcccctccac gtgtctcgcc cttggaacgg gtatcgacac
      301 tgatgggccc cagaccggca tgatagtgcc cctgccgatt ttggtcactt gcgagcgaca
      361 gtcctggtca agatcctgcg gaaagatgca caccagggct caagctgacg aaaccaacgc
      421 tacccgtgtg gccgatgacg ccggcttatt gattgaagcc aagagtgtct gccagttgtg
      481 tctgcgacat gccaacgact tggcggatct gcggcaggga aatgccctgc gcgtgatgca
      541 tgacgggcgc ctgccaccaa ctgctggatc cggacgcaag cgctcttggg ttcacgataa
      601 atctcgctcg gcctcgcgcc gtattccgcg ccttcgaaca atccgaaccc aaattttggg
      661 cgccaagcca cgggtaactc gacgttctcg acggcaagat caagccacca ctcgccgtcc
      721 cggctgcgag gctcatcaat gaaaactcta attcccggca actccgcttc cagcatgcgc
      781 ttgagctgct gcacaagatg cggaggtaga tccgtggaca cggagtccgc tgagctttgg
      841 gacttaggcc gaggatttga tgccatcgtc acacctctcc gattgatcgt ccgtcagagc
      901 cggaggacgt tcccctttag atccgtgatg agcagtgcgc cgtccgtggc aacgaaggtg
      961 ttatgcccga cgctccgata gcgcgcaaca tccgggcaat ctctgactct gcgcgcgctg
     1021 tccggctgcg gtagatacat ccgtccatct gtcgcgcaat tcgccgggtc atacggaatc
     1081 ggggtcattt cgtcctccag aacagatctc gagcaagtgg aatcgacact tcccaacatc
     1141 gtcattcccg cgaaagcgga atccaggggc tgccggcaca tcgctcgccc tgggtccccg
     1201 ctttcgcggg gacgacgacg gaggagcggt tctgcataaa ctggatccgc gctagagata
     1261 gggaagcatc agccttgcgc cgctgtcgat cagtttgacg gagggcggcc ggtcgtcgag
     1321 ttcggtgccg gtcatgagcg cgccccgtct cgcggccatc aaatcgtcga tgcgctggac
     1381 gatgtcggca tggtagacct caacggtcag ctcgaagttc agccgaaagc tgcgcacatc
     1441 ccagttcgcg ctgccgatca ggcaccagct gccgtcgacg gtcatcattt ttgagtggtt
     1501 gaagggtggc gggctgcgcc agattcgaca accggcggcg agcagtgggc cgatttgggc
     1561 acgcatggcc caatcgacgg caagatgatt gcctcgcgcc ggcacgatca cgtcgaccgc
     1621 gacgccgcgc atcgcggcca aagcgagcgc agtgatcagc ccgtcatccg gcagaaaata
     1681 gggcgtcgtg actttgatgg attggcgcgc gcaggcgatt gcttcaagga tcatgaactc
     1741 gatcttctcg agatcctcgt cggggccgga gacgacgaca cgcgcgacgg cctcgccgac
     1801 gcccgcgagc ggtggaaacc aggcgtcgcc gacgagattt tctccggcgg caaagaacca
     1861 gtccgcggca aaggcgtcga ccagctgcgc gacaaccggg ccttcgacgc ggaaatggac
     1921 gtcccgcacg ggatggcggg gcccctccgc caccaggttt tcgctgccga tattgagtcc
     1981 gcccgtgaac gcgacgcggc catccagaac gagaatcttc ttatgagagc ggaggttcag
     2041 gaacggcatg cgccacggca gcggtgaatg catgaagcgg aaaaccggga cttgatgtcg
     2101 gcgcagccgt cggtaggccg gcgaaagaaa gtagccgccg ccaatgccgt cgatcagaac
     2161 acggacctgg acgccacgcc ggcatgcgcg gaccagagcg tcgatgaagg cgccaccggc
     2221 atggtcggcg cgaaagatgt aggtcgacaa ggcgacgctg gtctcggccg cctcgatcgc
     2281 cgcgatcatt tgcggatagg cctcgtcgcc attgtgcagg acggcaacgg cgttgccgcg
     2341 ctcggcgggc tgccctgtga tccggtcccc ggcccgctcc agcgccgcca ggtgatcctc
     2401 gcgccccgcc ggcgagatcg gcggctcggc cacggggcgg gcggcgcgcg ggcgacgcag
     2461 ctgattggcg cgccgtttca cccggttgat gccgaatatg acatagagga cgctgccgat
     2521 gatcggcgag agccaggcga ggccgatcca gccgatcgag gcgccggcgt cacgtttgtg
     2581 gagcagcacg tgcaccgtga caccgatggc cagcaggata tgaagaaccg agagccccgt
     2641 ggcgtcgacc tgggcgaaaa ttgcgcgcac ccgtgactct cccctcgcaa tggcagtccg
     2701 catgagcacg ccctggaggg cgtccgttcg agcctctcac ccgtgcggcc aactggcgga
     2761 gaccggagac tcgatggccc tcgccgatga aattgcgccg aaagatagcc ccgttttcgt
     2821 ttccaggcct agccgggggg ttcccaaatc gatcatctgg aacggcgccg tagagctatt
     2881 ccccgggcga ggaggcctgg cgacatgtgc agcagtgcga cgatgccttc catgccgcgc
     2941 tcggccgcgt ctgctaccgc atcggtcccg cgcgcgacga tgttgagaat ggagtcggcg
     3001 gcgtcgatgt cggcgacgcg cgtcaagtcg atccccgcga gcatgctcgc tgacgatccc
     3061 gtgccggtga tgagagcgca gccttaccgc ccttcgcgtt caccatcgga cagtgcccgc
     3121 tccttgagca gattgctcgg aacttgatgc gggctcaccc gtccacaaat ctccacacgg
     3181 cccaggctaa ttccccggct aattctctcg cgtcagtaaa gtgaacgcgc tagtctggac
     3241 cctcagagaa tttcgcgtct gacgcgaatc ggtcggctgc cgagggggaa gggccgatga
     3301 cggcttctgg gaagcgacgg atcgccgtgc tggatggcgg ctcggcggca agggcgcgag
     3361 cgggcgcaac gctcaataca acgaccgcat cgaggagcac ggcctccatg tctgggccgg
     3421 cttctatgag aacgcgttcc agctgatgaa cgcggtccac aaggattacg cgccgccgtc
     3481 gtcgccggcc gcgcccttct tttgtttcgg ccccatgaat gacagcgcct gcccgacgtt
     3541 gagcgggccc ccaaggtcgt ccaggacggc gtcaccgacc tttcccttgg cgccagcggc
     3601 ttgatcttgt gcccagtgcg ccggccatct aggcttcaac aaaaggggga cacgggggcg
     3661 atgccggaaa gtcttttcga gcaggacttg acggcctatc tgcccggctt cctcgtgagc
     3721 atggagcaag gcgcggcgga gatgcggcgg gcgacgatgg cgcgcgccga gggcgccgtc
     3781 ctcctcgtcg acatcgtcgg cttcgtcgcc ctcgccgacc ggctcgcgga tcaggggcgg
     3841 cgtcgcgccg agacgctttc catccttctc gacgcctatt tcggccagat caccgacagc
     3901 atcctggcac atggcggcga cgtcattacc ttcgcgggcg acggcattgt cgcgacctgg
     3961 tcacgctcgg cgcatctcgc ggaaaatgcc cggcttgcgg cgcaatgcgc gatcgagctg
     4021 cagagttcgc tcgagccgct cgagcggcgg atgcgcctca ctttgcgcca gcgcgtcgtc
     4081 atcggggccg gcgagatcct gctctgcacg gtcggaggcg ccgtcagcag gcgctatgcg
     4141 ctcgccgcgg gcgccgctct cgacgaggcg gtgcgtttct accatcacgc gggacgcggc
     4201 gacatcctgc tcggtccgac gacacgacag gcgctcgcga caagcgccga gatcgaagag
     4261 agagacggcg tggcgttcct tgtgcgcatc ggcgctcgtt tgtcatcgcc gccctcgggt
     4321 gaggcgccgc agcaggagat cgcgacatcg cacttgacac cctatgtcgc gccggccgtg
     4381 gtcgagcgcg tgcgcgcggg ccagggcgcg tggctcggcg agtttcgcac cgtcaccacc
     4441 ttgttcctgg gcgtgggtgg cgtcgacgtt gctgggaatt ttctcgatgg cctccagaat
     4501 gcgacggaaa tcgtcgagcg cgagctcgcg cgcttcgacg gctcccttga ccgcgtttcg
     4561 atcgacgaga aggggctcgg ctacctcatc accttcggcg tgccgtcgaa ggcgcatgag
     4621 gacgatgcga tccgcgccct tcgcgccgcc ctcgccttgc gcgcggcact tcgcgcggcc
     4681 ggcctcgcgc cgtccatcgg cggcgcgacc ggcgagctct tctgcggccg ctatggcgga
     4741 agccggcgcc agacttattc cgttgtcggc gaagccgcga acatcgccgc gcggctgcgc
     4801 cagcacgtca atgacgacat catctgcgat gaagcgacct gcgcgctcgc ccgtaacgcg
     4861 atcgccttcg ccgatctggg cacgatcgag gtcaagggta aggccggcgc aatccgcatc
     4921 catcggccgc tccgcgaatt gctcgacaag ccgtggcgcg ccgagagcga gctcgtgggg
     4981 cgcgcggctg aacgcgcggc gctccgcgaa ttaatcgccg accgcgcttc gggcggcccc
     5041 atcatgatcg agggtgaggc gggaatcggg aagtcgcgtc tgctcgccga gatcgccgat
     5101 atcgctggcg agcacggcgc ctcggtgatc gccgccgccg gggcacagat cgaggaggcg
     5161 acgccctatt tcccctggcg ccgcgcgctt cgcggcctct tcgccgaggc cgagggcgag
     5221 gctctgcgcg cggcgatcct ggcgacgctg gccgatgacg accagcttcg cgtcctggcg
     5281 ccgctcctca acgacgtgct gccgatcggc atcgccgaga acgagctgac ccgcgagatc
     5341 gccggcggcg cgcgttcgtc gctcacccag cggctggtga tccggctctt cgagaaaagg
     5401 ctcggggagc ggcccctcat cctcgtcttc gacgacgcac actggttcga cgatgcctcg
     5461 gccgctctgc tccgacagct catgcacgct gtgcccgcgc tcgtcgtcgt catcgcgtcg
     5521 cggccgccgg ccaaaggcgc atccagcgcc ttcgtcccgc acctgggcga caagggcgag
     5581 cggcggatca cgctcggcgc gctcgagcgc cgcgcgatcg agcgcctcgt cgacctgaag
     5641 ctcggcgtca ccgccgcgcc cgcgccgctc tacgacttcg tcttccgccg cgccgcgggc
     5701 catcccctct ataccgagga gctggtgctg gggctgcgcg ccggcggctc gctcgagatc
     5761 g
//
LOCUS       DASYNS010000200         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_979240, whole genome shotgun sequence.
ACCESSION   DASYNS010000200 DASYNS010000000
VERSION     DASYNS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979240"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VGP50_06165"
     CDS             complement(<1..455)
                     /locus_tag="VGP50_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HEV8026994.1"
                     /translation="MADATAVPSVNALSAPLVRRLVEDAPALRLGVSRDPSGATLVDA
                     GINAAGGIEAGRRIAEICLGGLGRVTLAAGEAWPSMVTVHTANPVLACLGCQYAGWSL
                     QHEEKGKRFFALGSGPGRSLAGKEELFGELAYRDQAAETALVLEVDRPPP"
     gene            complement(468..1319)
                     /locus_tag="VGP50_06170"
     CDS             complement(468..1319)
                     /locus_tag="VGP50_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV8026995.1"
                     /translation="MLGSRIAILGNRAEVVGAVKDPCGFAALLARLGVPHPETRLAAP
                     ADGNWLAKRVGGSGGGHITCASAGAIAAGFYVQRQIAGRAVSALFVGDGRRARVLGHS
                     EQWTSPTAEMPFRYGGCVGPVRLGAKIEAMIDELCQELVAALGLVGLNSLDLLLDGER
                     FHVLEVNPRPGATLDVFDGRDGLSFWDAHIAGVAGRLVPFHGGMGPPRAAAVVYAPAR
                     LRIPLSFAWPDSAADRGRAGTLIESGEPVCTVMATAATPAEAREIVDMRARRLLERLP
                     PLPLAST"
     gene            complement(1645..>2107)
                     /locus_tag="VGP50_06175"
     CDS             complement(1645..>2107)
                     /locus_tag="VGP50_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydromethanopterin dehydrogenase"
                     /protein_id="HEV8026996.1"
                     /translation="AEAGAHVVLVAHDKSGIVEAKAAEFERRFGRSFAVADGSSEASR
                     RTVLKNAEVVLACGRAGLQILSRAEMSAAQRLKVAADINAVPPPGIEGLDVKANGTKL
                     PGTPGMGIGALAIGNVKFQLQHALLKRLDEAQKAQCFDFRDAYRVAIELVH"
BASE COUNT          343 a          744 c          714 g          306 t
ORIGIN      
        1 ggcggcggac ggtcgacctc gagcaccagc gccgtttcgg ccgcttggtc gcgataggca
       61 agctcgccga agagctcttc ttttccggcg aggcttcgcc cgggaccgga gcccagcgcg
      121 aagaagcgct tccccttctc ctcatgctgc aggctccagc cggcatattg gcagccgaga
      181 caggcgagca ccggattggc cgtgtgcacc gtcaccattg acggccaggc ctcgcccgcg
      241 gccagcgtga cgcggccgag cccgccgagg cagatctcgg cgattctacg cccggcttcg
      301 atgccgccgg cagcgttgat cccggcatcg accagcgtcg cgcccgaggg gtcgcggctg
      361 acgccgaggc gcaacgcggg cgcgtcctcg acgaggcgcc ggacgagagg cgcactcagg
      421 gcgttgacgc tcgggacagc ggtcgcatcg gccatggtcg gctcctttca ggtcgaagcg
      481 agcggtaacg gcgggagccg ctcgagcagg cggcgcgccc gcatgtcgac gatctctcgc
      541 gcctcggccg gcgtcgccgc cgtcgccatc acggtgcaga cgggctcgcc gctttcgatg
      601 agcgtgccgg cccggccgcg gtcggcggcc gagtcgggcc atgcgaagga aagcggtatc
      661 cgaagccgcg cgggggcata gacgacggct gcggcccggg gtggacccat tccgccatgg
      721 aacggcacga ggcggccggc aacgccggcg atatgcgcgt cccagaacga cagcccgtcg
      781 cggccgtcga agacatcgag cgtggcaccc ggccgcggat tgacttcgag cacatgaaag
      841 cgctcgccgt ccagcaggag atcgaggctg ttgagcccga cgagaccgag cgccgccacc
      901 agctcttggc agagttcgtc gatcatcgcc tcgatcttcg cgccgagccg caccggtccg
      961 acacagcctc cgtagcggaa cggcatctct gcggtgggag acgtccattg ctcgctgtgg
     1021 ccgagaactc ttgcccgtcg accgtcgcca acgaacagcg ccgataccgc gcgaccagcg
     1081 atctgccgct ggacgtaaaa tcctgcggcg atggcaccag cggacgcgca cgttatgtgg
     1141 ccgccacccg atccgccgac gcgcttcgcg agccaattcc catctgccgg cgctgcaagc
     1201 cgcgtctccg gatgtggcac gccaaggcgt gcgagcagcg cggcgaaacc gcagggatcc
     1261 ttcaccgcac cgacgacctc cgctcgattg ccgagaatgg cgatgcgcga tcccagcatc
     1321 tccagcagcg cgggctcatg ctcgaacccg gcgccataga cgagccccga tgctctacca
     1381 cggtgctctt cgaccgcctc gagaagtgcc gccccatcga atgcgagaca tcttcccctc
     1441 tcccctggtg ggagagggag gggcccatcg cgcagcgatg ggagggtgag gggagctgac
     1501 gccgaaatac cgcgagcgag ggtcgcaagt tccgccgtat cctcatcggc aaacaagtcg
     1561 agcgcgatca cgcgtgcgcc ggcacggcgc gctgccgcgg cgagaggacg cgccgagagc
     1621 gcgacggcga gcgccggttg cgcctcagtg gacaagctcg atcgccacgc gataggcatc
     1681 gcgaaaatcg aagcactggg ccttctgcgc ctcgtcgagg cgcttcagca gcgcatgctg
     1741 cagctggaat ttcacgttgc cgatcgccaa ggcaccgatg cccataccgg gcgttccggg
     1801 cagcttcgtc ccgttggcct tgacgtcgag gccctcgatg ccgggcggcg gcacggcgtt
     1861 gatgtccgcc gccactttga gtcgctgtgc ggcgctcatt tccgctcgcg agaggatctg
     1921 gaggccggca cggccgcagg cgaggacgac ctcggcattt ttcagcacgg tgcgccggct
     1981 tgcctcgctg ctgccgtcgg ccaccgcaaa ggagcgcccg aagcgccgct cgaactccgc
     2041 cgctttcgcc tccacgatgc cgctcttgtc gtgagcgacc agcacgacat gggcgccggc
     2101 ctccgcg
//
LOCUS       DASYNS010000201         2422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2853294, whole genome shotgun sequence.
ACCESSION   DASYNS010000201 DASYNS010000000
VERSION     DASYNS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2853294"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VGP50_06180"
     CDS             complement(<1..628)
                     /locus_tag="VGP50_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEV8026997.1"
                     /translation="MAAPPRPRRSVLYMPGSNTRALEKARGLPADALILDLEDAVAPD
                     AKAMARANVASALRAGGYGRREIAVRVNGLATPWGYEDLGAAATMKADAVLLPKVESA
                     DTVRQAEALLAAAGASASLALWCMMETPLAMLRAEAIASASPRVTCLVMGTSDLTKDL
                     QARHTRDRLPMMTSLGLCLLAARAYRLAILDGVNLDLGDDAGFAEVCRQ"
     gene            726..1571
                     /gene="pdxY"
                     /locus_tag="VGP50_06185"
     CDS             726..1571
                     /gene="pdxY"
                     /locus_tag="VGP50_06185"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HEV8026998.1"
                     /translation="MAILSIQSAVAYGRVGNSAAVFPLERLGHEVWPVDTVQFSNHTG
                     YGAWRGRAWDAAAIAEVIRGIAERGVFSSCAAVLSGYLGEPALGDVVLDAVRQVKAAN
                     PAAIYCCDPVIGDDHRGIFVRPELVAFFRDRALPAADIVTPNKFELALLTGRTIAGTA
                     DALAAAAQIRARGPRWILATSLPGTTVDAVAMLAAGADGAWLVETPRLPIVANGAGDL
                     TAALFLARFLQSRDPATSLGDTASAVHAVLEETVRRGAAELQIVAAQHALATPPSRFS
                     SRKIA"
     gene            1731..>2422
                     /locus_tag="VGP50_06190"
     CDS             1731..>2422
                     /locus_tag="VGP50_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEV8026999.1"
                     /translation="MSRAYASPVTLSERTVRAGRLALRGQAKGFGSFLPFVGPAVIAS
                     VAYMDPGNFATNIEAGAKLGYDLLWVVVLASVIAMFFQALSAKLGIATGRSLAQASRD
                     CFPRPVVLAMWVVSEIAAMATDLAEFLGGAIGLSLLFHLPLLIGMAVTGVATYALLML
                     QGRGFRPIELVIGGLVAVIGVSYLVELVIVPPDWAAAAYHAVVPTLSGMDALTLAVAI
                     VGATVMPHAIHV"
BASE COUNT          347 a          853 c          825 g          397 t
ORIGIN      
        1 cctggcggca gacctcggca aagcccgcgt catcgccgag atcgagattg acgccgtcga
       61 gaatggcgag acgataggcg cgcgccgcga ggaggcagag ccccagcgaa gtcatcatcg
      121 gcagccggtc gcgcgtgtgg cgcgcctgaa gatccttggt gaggtccgag gtccccatga
      181 cgagacacgt gacgcgcggg ctcgccgagg cgatcgcttc ggcgcgcagc atggcgagcg
      241 gcgtctccat catgcaccag agggcgaggc tcgccgacgc gccggcggcg gcgagcagcg
      301 cctccgcctg acgcacggtg tcggcgctct cgaccttcgg cagcaatacg gcatcggcct
      361 tcatcgtcgc cgcggcgccg aggtcctcat aaccccaggg cgtcgcgagt ccgttgacgc
      421 gcacggcgat ctcgcgccgc ccatagccgc cggcgcgcaa cgcgctggcg acattcgccc
      481 gtgccatcgc cttggcatcg ggcgccacgg cgtcctcgag atcgagaatg agcgcatcgg
      541 ccggcagtcc gcgcgccttc tcgagcgcgc gggtgttgga gccgggcata tagagaacgc
      601 tgcggcgcgg gcgcggagga gcggccatgg gtgatctccc ttcgggtcgg acgccactat
      661 agcgacacgc cgcggacgag acagccattc gcgaccgcgg tgtcgcttgc cctatcattg
      721 ccccgatggc gatcctatcg atccagtcgg cggtggctta tggccgtgtc ggcaactccg
      781 cggcggtgtt tccgctggag cgcctcgggc acgaagtctg gcccgtcgac acggtgcagt
      841 tctcgaatca tacgggctat ggcgcgtggc gcggccgcgc ctgggatgcg gccgccatcg
      901 ccgaggtgat ccgcggcatc gccgagcgcg gcgtcttctc gagctgtgcg gccgtgctct
      961 cgggatatct cggcgagccg gcgctgggcg atgtcgtgct cgacgccgtc cgccaggtca
     1021 aggcggcgaa cccggccgcg atctattgct gcgaccccgt gatcggcgac gaccaccgcg
     1081 gcatcttcgt gcgacccgag ctcgtcgcct tcttccgcga ccgcgcgctc cccgccgcag
     1141 acatcgtcac gcccaacaaa ttcgagctcg cgctcctcac cggccggacg atcgccggca
     1201 ccgccgatgc gctcgccgct gcagcgcaga tccgcgcgcg cggcccgcgc tggatcctgg
     1261 cgacgagcct tcccggaacg accgtggacg ccgtcgccat gctggcagcg ggcgcggacg
     1321 gcgcctggct cgtcgagacg ccgcggctgc cgatcgtcgc caacggcgcg ggcgatctca
     1381 ccgctgcgct cttcctcgcg cggttcctcc aatcgcgcga tcccgcgacc tccctcggcg
     1441 ataccgcgag cgccgtccat gccgtcctcg aagagaccgt ccggcgtggc gcggccgagt
     1501 tgcaaatcgt tgcggcgcag cacgcattgg cgaccccgcc gtctcgcttc tcgtcgcgaa
     1561 agatcgcgta gggaacaccg gatcggggtg gatgcacgcg ccttttcgag gagtcgtttg
     1621 acggaattct cagggcatcg cggcgaaaag ccggctagga tcaccctcaa caagcgatag
     1681 cggcggtcaa gatcgcgtcg ccgacaaaga gcgaagcgcg gggagcaacg atgtcgaggg
     1741 cctacgccag tcccgtgacg ctgtccgagc gtaccgtccg ggcgggccgt ctggcgctcc
     1801 gcgggcaagc caaagggttc gggtcgttcc tgccctttgt cggccccgcc gtcatcgcct
     1861 ccgtcgctta tatggacccc ggcaatttcg cgacgaatat cgaagcgggt gccaagctcg
     1921 gctacgacct cctttgggtt gtcgtgctgg cgagcgtcat cgccatgttc ttccaggcgc
     1981 tctccgccaa gctcggaatc gcgaccgggc gcagcctggc gcaagcgagc cgcgattgct
     2041 tcccgcggcc ggtcgtgctg gccatgtggg tcgtcagcga gatcgcagcc atggcgaccg
     2101 atctcgccga gttcctcggc ggcgccatcg ggctctcgct gctgttccac ttgccgctgc
     2161 ttatcggcat ggcggtgacc ggagtcgcca cctatgcact cttgatgctg cagggacgcg
     2221 ggttcaggcc gatcgagctg gtgatcggcg gcctggtcgc cgtcatcggc gtgagctacc
     2281 tcgtcgagct cgtcatcgtg ccgcccgact gggccgcggc ggcgtatcac gccgtggtgc
     2341 cgacgctctc gggcatggac gcgctgaccc tcgccgtcgc catcgtcggc gcgacggtga
     2401 tgccgcatgc cattcacgtc ca
//
LOCUS       DASYNS010000202        23135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1761574, whole genome shotgun sequence.
ACCESSION   DASYNS010000202 DASYNS010000000
VERSION     DASYNS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 23135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23135
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1761574"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VGP50_06195"
     CDS             complement(<1..1317)
                     /locus_tag="VGP50_06195"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEV8027000.1"
                     /translation="MTVWLGMAGALGLLPKPAYAAESAAATLLPYILGATLVALAATA
                     AVAIFRLRHRLQRLAAERDETLAALAENRAVLGATPAALFRWNCRDGSEIFTSGPLSL
                     LGDAGPARFADLLARVAAKDRAALEAAVAQLRDDGTAFQLNLALVDGGFTLDATGRRA
                     RRGDGTALSDLVWLADGSRSAEAIAARDQNAQESRRLRAILDALPLPVWQRGPDLELL
                     DCNRAYGAAVDTTWSKALAEQREVAAGVVGENGRAIASRARDTLVTQSESHHIVIAGS
                     RRLMEFTEAPLDGTDDFVGYARDFTDVENTQSELARHIAAHADVLENVAVAIAIYGPD
                     NRLTFFNTAFATLWRLEEDWLVTEPSLDELLERLRERRRLPEYADFRAFKRQQLGMFT
                     SLIEPQEELLHLPDERTLRLVVSPHPFGGLTFVYEDVTDKLALERSY"
     gene            complement(1442..2737)
                     /gene="ahcY"
                     /locus_tag="VGP50_06200"
     CDS             complement(1442..2737)
                     /gene="ahcY"
                     /locus_tag="VGP50_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEV8027001.1"
                     /translation="MAASADYKVADIALADWGRREIAIAETEMPGLMALRREYGAKKP
                     LKGARITGCLHMTIQTAVLIETLMELGATVRWSSCNIFSTQDQAAAAIAARGVPVFAW
                     KGETEEEYEWCIEQTLRGPNDWKPNMVLDDGGDATKIVHEKHPELLNDIRGISEETTT
                     GVHRLYEMMKKGTLKVPAFNVNDSVTKSKFDNLYGVRESLVDGIKRATDVMIAGKVAV
                     VCGYGDVGKGSAESLRSQGAQVIVTEIDPICALQARMEGYQVLTMEDVAPIGDIFVTA
                     TGNIDVITLDHMRRMKDRAIVCNIGHFDSEIQIAALHNYRWEEVKPQVDEVVFPDGKR
                     LIVLAKGRLVNLGCATGHPSFVMSASFTNQVLAQIELYTNQGRYENKVYVLPKQLDEK
                     VAALHLEKLGVKLTKLDAKQAAYLGVGEKGPFKPERYRY"
     gene            complement(2845..5763)
                     /locus_tag="VGP50_06205"
     CDS             complement(2845..5763)
                     /locus_tag="VGP50_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV8027002.1"
                     /translation="MQLTEDSQAVPGASEIELAIDPDEIALSFGEHLLLPRRRQLLLG
                     STPVELGSRAFDTLLVLVEAEGRVVSNDELLRRVWPGMVVDKTNVTVQVSAVRKALGN
                     GRDLVKTDAGRGYRFTAAVRKIANAAEAIPKLSHVKALTEATTNLPTPISSLVGREHD
                     LKAVLYRVTTQRLVTLIGPGGIGKTQLALRAARLALPHFADGAWMVELDALKDAELVP
                     HAIARVVGVPSRSNHNLVDQIIAALAPKHLLLVIDNCEHVIGAVAQLAELLLRGAPQL
                     HVLTTSREPLVAEGEHLYPVRPLTFPATDTSNVATAIEHSAVQLFAERAQAANPLFVL
                     DDCTMPGVSKICRRLDGIPLAIELAAARVATIGVDMLARRLHDRFQLLTDGRRTAMWR
                     HQTLSAALDWGYELLSAPERTVLRRIAIFAGSFTLDSAGVVAAGDGLDPAQASDMVMR
                     LVQKSLVTFDVRRSVARYRLLDTTRAYALAKLAESGEFAMAARGHADHLRALLEAAEA
                     AWQTTPASELIAKFAPEVDNIRAALEWAFDSDGDAEIGVALAAAAVPLWTLLSILGEC
                     RDLVDRALRHLAKGANRQSRHEMRLQAALGRSSMWAKAAVGEAGSANTRALDLAERLG
                     DAEYQLTALYNLWICQLRIGAHRMSLAIAERFRQVAEAEGDVAAVRTGGRMEGVSLFN
                     LAEYARARRALETVIADQDASVRRSYVLRFGVDQRVSALSCLARILWLQGFPDQAARV
                     AQAAVEEARIVNHVNSFCVALCFGACGLATMSGEPRAVEEFAPLLTDGAEKHGLGMWE
                     VDSMALKGWMAIKRGDALVGLELMTRALADFKQGRLGLHRPIFVGAMAEALSAVGRAA
                     EGLATIEQALAEASRDEAGWCLPELLRVRGELLLRCALPDASLAAERDFHDSMALAGR
                     QGARSWQLRTATSLARLWRAQGREGAAGEMLRPIYEIFTEGRDSIDHRTAQALLATLP
                     "
     gene            6125..6349
                     /locus_tag="VGP50_06210"
     CDS             6125..6349
                     /locus_tag="VGP50_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV8027003.1"
                     /translation="MQAVLGRRLRWLRVERGFSRKTVAARLGLPVTLVERHERGMARL
                     EAQQLAAYARFYCIRISFLFRDPPAAAAGA"
     gene            complement(6389..8182)
                     /gene="ptsP"
                     /locus_tag="VGP50_06215"
     CDS             complement(6389..8182)
                     /gene="ptsP"
                     /locus_tag="VGP50_06215"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV8027004.1"
                     /translation="MKNEPPPFAGDKPGKRERVLHGLGVSPGIALGPAFVSDHGDIAV
                     PERRIAAGEVEAEGERFAAALSLSLKQLRKLKGKSSALPDSAAEEVGYLLDAHIAMLT
                     KSRLVRGVERRIREERINAERAVQMEIAQISESFAAMGDAYLAARVDDIRVVGARLIR
                     NLTKTPYAALQHVPEGTVILAEELTPADTALMDPQRIAAFAAVLGGAESHTAIMARAL
                     GLPAVLGVTGLLGQIDAGDMVLIDGASGTVVINPTRETVVSFEAQREVLALERRQLAR
                     LKRLPAVTRDDVEIVLQANLELPRELEQAIAAGAAGIGLVRTEFLYMNREDLPDEDEQ
                     YEAYRGLVRGMDGRSVTIRTLDIGGDKLAAPLAEALEGVGANPALGLRAIRLSLKERP
                     LLDAQLAAILRAGADGPVRILLPMVCSVAEVLKVREALGQVVRRLKRRGLKIADPLPP
                     LGVMIEIPGAALAADALAAACDFFAIGTNDLIQYTLAIDRGDEQVAHLYNPLHPAVLR
                     LIQFAVEAALRARLPVSLCGEMAGDPRYTALLLGLGIRDLSMAPAKIGLVKRRIRTLD
                     MVAAGRRARAIMDQLDDDRIASLLDDFNALA"
     gene            complement(8172..8480)
                     /locus_tag="VGP50_06220"
     CDS             complement(8172..8480)
                     /locus_tag="VGP50_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEV8027005.1"
                     /translation="MNDTGEPSAADGAAPIRRTVTICNKRGLHARAAARFVKLAWQFD
                     AEVFVAKNGAAVSGRSIMGLMMLAAGPGTDIELRASGPEAERAIGALAELIAGGFDEK
                     "
     gene            complement(8477..8884)
                     /locus_tag="VGP50_06225"
     CDS             complement(8477..8884)
                     /locus_tag="VGP50_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV8027006.1"
                     /translation="MIGMVLVTHGRLAAEFVAALEHVVGPQRNIAAVCIGPDDDMEKR
                     RQDILRCVEEVDAGQGVVLLTDMFGGTPSNLAISVMDKAKIEVIAGINLPMLIKLASL
                     RPTETLANAVRGAQEAGRKYINVASQLLADVRG"
     gene            complement(8881..9837)
                     /gene="rapZ"
                     /locus_tag="VGP50_06230"
     CDS             complement(8881..9837)
                     /gene="rapZ"
                     /locus_tag="VGP50_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV8027007.1"
                     /translation="MSDKLAPKFEASTPAASRLLVVTGMSGAGRSTALKALEDLGYEA
                     VDNLPLSLLPSLLEMPAERPPLAIGIDVRTRDFGVGPLVAALDRVVAEHHLELQIVFL
                     DCEDDWLVRRYTETRRRHPLAGDRPVIDGILLERQRVSPLRDRADLVIDTTALNPSDL
                     KRVLHGHFALNAAPGIAIFVTSFSYRHGLPRDADLVFDARFLRNPHYVAALRPLTGRD
                     PEVAAFIAADADFAPFFERLCQWLAPLLPRFEREGKSYLTIAFGCTGGRHRSVYLAER
                     VAEWLGEQGRPVGVNHRDLDQSATLLATGTNAAAPKPVKDIA"
     gene            complement(9834..10280)
                     /locus_tag="VGP50_06235"
     CDS             complement(9834..10280)
                     /locus_tag="VGP50_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HEV8027008.1"
                     /translation="MIRVHGTSVVLGGDGVLLRGPSGSGKSDLALRLIDQGARLVADD
                     QTELRFIGGALRLSAPSTIAGQIEVRGVGILRVDSVKSAPLRLVVDLVANDAVERLPE
                     PLTCEFLGCRVPRLRLAPFEASAAAKLRLALRALPVLARASLPLTS"
     gene            10566..10736
                     /locus_tag="VGP50_06240"
     CDS             10566..10736
                     /locus_tag="VGP50_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027009.1"
                     /translation="MHATTTSTDPEAQDRTSRLRKQILKLRWIGMESEAAELARSAAC
                     PDILLREIAERD"
     gene            complement(10758..12452)
                     /locus_tag="VGP50_06245"
     CDS             complement(10758..12452)
                     /locus_tag="VGP50_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HEV8027010.1"
                     /translation="MGSDIATATAEPRPQPPAVAPPAPAFAPDRPRRPRLSPLTRQIL
                     TVNVLALGLLVFGLLYLGEYQRSLIAAELDALRTQGQIFAAALGEGAVIDSLGEGETL
                     IPELGRQMMRRLVEPTHTRARLFDAQGKLIADSRVLRGPGGMVQINELPPPGERGALD
                     RLSEWLYDALLGLLPGQKHYPPYEEAAAQSAGDYREVQQALLGEVENAVRRDRDSSGL
                     VLSVAVPVQRYKQVLGAVMLSTDSSEIDQTLRAARFEILKWFAVALSVTVLLSIYLAG
                     TIARPIRRLAAAAERVRRGHGRQVPIPDFTRRGDEIGDLSGALREMTSALWQRMDAIE
                     NFAADVAHEIKNPLTSLRSAVETAARVDDPQKQRKLLSLVLDDVQRLDRLITDISDAS
                     RLDAELSRVELEPVLVGPMLETLVDVHQSTSDEKAQRLVLALPGPVGTPDGDLRVLGI
                     EGRLVQVFRNLIANAISFSPPAAAIHIAAKRDGDAVVVTIEDEGPGMPDGKLEAIFER
                     FYTERPAGEKFGTHSGLGLSISKQIVEAHRGTIRAENRRDVDGSVIGACFIVRLPG"
     gene            complement(12421..13113)
                     /locus_tag="VGP50_06250"
     CDS             complement(12421..13113)
                     /locus_tag="VGP50_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV8027011.1"
                     /translation="MANTIALVDDDKNILTSVAIALEAEGFQVRTYSDGAEALRGLAA
                     HPADLAILDIKMPRMDGMEVLGHLRKQGNMPVIFLTSKDDEVDEVLGLRMGADDYIKK
                     PFSQRLLIERIRALLRRGDLARGKGESEPVVTRGELVLDPGRHQCSWQGHNVDLTVTE
                     FLILKSLALRPGHVKSRDQLMDGAYGEHIYVDDRTIDSHIKRLRKKFKVIDQSFAQIE
                     TLYGVGYRYRDR"
     gene            13358..14950
                     /locus_tag="VGP50_06255"
     CDS             13358..14950
                     /locus_tag="VGP50_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027012.1"
                     /translation="MKSFMRGLRRAAMLALGAAMIVPQAHAQSAAKTIKFIPEADLRS
                     LDPIWTTAYITRNHGYMVYDTLFALDEHLQPRPQMVDRWTASEDRLTYTFTLRDGLKF
                     HDGAPVRSADCIASLQRWMKRDGLGQVLGDAMAEMPVTDDKTFAIVLKRPFPLLLEAL
                     GKLSSNVPFIMPERIARTDPNTQITDPTGSGPFKFVKEEWIPGNKAVYVKNTDYVPRA
                     EPPSWAAGGKVVKVDRVEWLYIPDSATAAAALNAGEVDWWQQLPPDLVPLLERNKDVT
                     VANVDPLGSIGVMRFNQLQPPFDNAALRRAVLYAIDQQDIMAALGGDAKNWKTCYSVY
                     TCGTPLASETGADALKGKRDLDKAKQLVKESGYKGERVVVMTATDQPIVNAQANVIAE
                     ELRQIGLNVDLQAMDWGTLITRRASREPVDKNGWSIFCTWLVGPDMINPALSFPLRAN
                     GAKAWFGWPTDDKIEALRAQWIEAPDLGTQQKLAAALQEEAFTSVPFVPTGQFVIPTA
                     FRKSLKGVVPAPVVFLWNVEKS"
     gene            15120..16061
                     /locus_tag="VGP50_06260"
     CDS             15120..16061
                     /locus_tag="VGP50_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8027013.1"
                     /translation="MLAYILRRLAATVPVMGVVAVAVFSLLYIAPGDPAAMIAGDLAT
                     ADDIARIHQQLGLDKPFVIRFGGWLWGVLHGDLGTSIVSSQPVAVLIGQRIEATAALT
                     VMTLLLSIAFAIPLGVLAAWRVGTWIDRSVMVLAVLGFSVPSFVLAYLLILGFSVELN
                     LLPVQGYVSIREGFAPFIAHLILPSIALGLIYGALIARITRASMLEVLSQDYIRTARA
                     KGLADNVVLRRHALRNAAIPIVTVIGIGLGLLISGVVVTETVFAIPGIGRLTVDAILR
                     RDYPVIQGVILLFSFAYVLVNLAIDISYTLLDPRVRY"
     gene            16072..16938
                     /locus_tag="VGP50_06265"
     CDS             16072..16938
                     /locus_tag="VGP50_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8027014.1"
                     /translation="MRAELLAPASGRHWLGDAVRRHPTVAAGGLLLLAMAAAAILAPW
                     LGTVDPLHGEVVKRLRFPSAQSWFGTDAFGRDVYSRTVFGARVSLEIGLGVALFSTAL
                     GLVIGLVTGFSRIADAIVMRIMDGLMSIPPVLLAIALMALTHASIGNVMIAITVAEVP
                     RVTRLVRGVVLTLREQPFVEAAVAAGSGLLRILRRHILPNTLAPVLVQATYICASAMI
                     TEAVLSFIGAGTPPEIPSWGNIMAEGRSFFQIRPYLVLFPGVFLSLTVLAVNLVGDGL
                     RDALDPRLARRL"
     gene            17052..18062
                     /locus_tag="VGP50_06270"
     CDS             17052..18062
                     /locus_tag="VGP50_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027015.1"
                     /translation="MGGDAAHISPLLEVSDLRTHFFTRDGVVRAVDGVSFAVNAGETL
                     AVVGESGCGKSVTALSILRLVPDPPGRIVGGALRFAGRDLLRLTESAMRDVRGNEISM
                     VFQEPMTSLNPVLTLGRQIGETLMRHQGLDARAANARAIDMLRLVQIPEPERRARQYP
                     HELSGGMRQRVMIAIALACNPKLLIADEPTTALDVTIQAQILDLMRALKARIGAAIIL
                     ITHDLGVVAEMAERVVVMYAGRKVEEAAVRALFRRPRHPYTLGLLAAVPRLGATLGRE
                     PAPLAEIPGVVPSLREKIIGCAFAPRCAYVTPRCRSEAPSLEAKAPAHLAACFESERL
                     PS"
     gene            18059..19048
                     /locus_tag="VGP50_06275"
     CDS             18059..19048
                     /locus_tag="VGP50_06275"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEV8027016.1"
                     /translation="MSAQSTPLLEVVGLVKHFPVRRGLFSRRTGAVRAVDGISFAIAA
                     GETLGLVGESGCGKSTAAKTILKLIEPTAGTVRLRGERIDHLPPRAMRHWRRELQVVF
                     QDPYSSLNPRLRAGDIVAEPLVNFGVMRGAALRDRVAGLFAQVGLSADAMRRYPHEFS
                     GGQRQRIGIARALALDPSLVVCDEPVSALDVSVQAQVINLLKELQRERHLSYLFVAHD
                     LAVVEHISHRVAVMYLGKIVELAPKDALFSAPLHPYTEALLSAVPQPDPETKRNRIIL
                     RGDVPSPIDPPPGCRFHTRCLYAEPRCRIDEPELREVAPGHRVACHLRPVAAM"
     gene            complement(19082..20548)
                     /locus_tag="VGP50_06280"
     CDS             complement(19082..20548)
                     /locus_tag="VGP50_06280"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEV8027017.1"
                     /translation="MARYDLVLRGGSVIDGTGAPAITADVAIVGDRIVAVEGLPPASG
                     AAEVDVDGLAVAPGFIDVHTHDDRALLSRGDMAPKASQGVTTVVVGNCGVSLAPMLAG
                     RPLPPPLDLIGDAEHFRFRRFGDYLAALDQAPAAANAASLIGHSTLRACVMDRLDRPA
                     SESEIAAMREHLAEALEAGAIGLSSGLAYAPARAAPEAEVVALAQTLRDAGAIYTTHM
                     RDEGDHVLDSLEESFAVGRAAGIPVVISHHKVLGTQNFGRTRETLPRIAAAMAEQPLA
                     LDAYPYIASSTVLLAENIPRAAKILITWSKPHPKASGRTLADIARDWGASEAEAAARL
                     QPAGAIYFMMSEEDVRRVLAFPHTMIGSDGLPHDRHPHPRLWGTFARLLGHYCRDAKL
                     FPLEEAVRRMTGLPAARFGLSGRGHVVPGAYADITVFDPATIIDRASFEAPTTPAAGI
                     HLVLVNGVAVWSDGAATGARPGRALRRQRLRQETGGTA"
     gene            20702..21418
                     /locus_tag="VGP50_06285"
     CDS             20702..21418
                     /locus_tag="VGP50_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HEV8027018.1"
                     /translation="MATERADTPAATARRLMRALDRATLATSLEGWPYASLVLVALDH
                     DASPLLLLSDLAEHSKNLARDRRASLLFDGTAGRDDPLTGARVTVLGEIQPIADERLL
                     SRFTSRHPSAAGYAGFADFRLYRLTVARAHLVAGFGRIHWIAADALLSPAAPALAEAE
                     PGILEHMNKDHGEVIDLYATRLAGRTGQGWRLIGIDPEGIDLRLGGTVERLDFREPVR
                     DARSAREALVQLAQQAREQS"
     gene            21552..>23135
                     /locus_tag="VGP50_06290"
     CDS             21552..>23135
                     /locus_tag="VGP50_06290"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEV8027019.1"
                     /translation="MQDAAHTVTGKPSSFGLERQGLHHVAAVHWNLSVPILYEHAIRR
                     REGLVAAGGAFVARTGAFTGRTPRDKYIVEEPSSKADVWWGDINKPVSEATFESMYRR
                     LTAYFQRRELFVQDLYGGADPNYRLKVRVVTDSAWHSLFVRNMFIRPKAEALGDFQPD
                     FTILHAPHFFADHVADGLNSDTFIFVNFARRLVLIGGSEYAGEIKKSVFGYLNFLLPA
                     RDVLPMHCSANIGPKGDSAIFFGLSGTGKTTLSADGARMLVGDDEHGWSHHGLFNFEG
                     GCYAKVINLSSEAEPEIYATIHRFGTVLENVILDPASHMPDLSDGSLTENTRACYPLD
                     FIPNASHTGMAPHPDNVIMLTADAFGVLPPIAKLTPEQAMYHFLSGYTARVAGTEKGL
                     GNAPQATFSTCFGAPFMPRHPSVYAKMLGERISEHGAQCWLVNTGWSGGAYGVGSRMK
                     IAHTRALVRAALAGTLKDAPVKQEPYFGLYVPESCGDVPRDVLNPRHTWSDKGAYDET
                     ARELTKRFESNFTKFEAYVG"
BASE COUNT         3721 a         7994 c         7677 g         3743 t
ORIGIN      
        1 ataggaacgc tcgagcgcca gcttgtcggt cacgtcctca tagacgaaag tgagcccgcc
       61 gaagggatgc ggcgacacga cgaggcggag cgtgcgctcg tccgggagat gcagcagctc
      121 ctcctgcggc tcgatgagcg aggtgaacat gccgagctgc tggcgcttga aagcgcggaa
      181 atcggcatac tccggcagcc gccgccgctc gcgcagccgc tccagcagct cgtcgaggct
      241 gggctcggtg acgagccagt cctcctcgag ccgccacagc gtcgcgaagg ccgtgttgaa
      301 gaaggtgagg cggttgtcgg gaccatagat ggcgatcgcc accgccacat tctcgagcac
      361 gtccgcatgc gccgcgatgt ggcgcgcgag ctcggactgg gtgttctcga catcggtgaa
      421 gtcgcgggca taacccacga aatcatcggt gccgtcgagc ggcgcctcgg taaactccat
      481 gagccggcgc gagccggcga tgacgatgtg atggctctcc gactgggtga ccagcgtgtc
      541 gcgggcgcgg ctggcgatcg cgcgcccgtt ctcgccgacg acgccggcgg cgacctcgcg
      601 ctgttcggcc agcgccttgc tccaggtggt gtcgaccgcg gcgccatagg cgcggttgca
      661 gtcgaggagc tcgagatccg gcccgcgttg ccagaccggc agcggcaagg cgtcgagaat
      721 ggcgcgcagg cgccgagatt cctgcgcatt ctggtcgcgc gcggcgatcg cttcggcact
      781 gcgcgaaccg tcggccagcc agacgagatc gctgagcgcc gtcccgtcgc cgcgacgggc
      841 gcgacgaccg gtcgcgtcca gggtgaagcc gccatcgaca agtgcaagat tcagttgaaa
      901 agcggtgcca tcatcgcgca actgcgcgac ggcggcctcg agcgccgccc ggtccttggc
      961 cgcgacccgc gcgaggaggt cggcaaagcg cgccgggccg gcatcgccga gcagcgacaa
     1021 cggcccggac gtgaagatct cgctgccgtc ccggcaattc cagcggaaga gcgcggcggg
     1081 ggtagcgccc aagacggctc ggttctcggc caacgcggcc agcgtttcat cacgctcggc
     1141 ggcgaggcgc tgcagccggt gccggaggcg gaagatcgcg accgcagcgg tagcggccag
     1201 cgccaccagg gtcgcgccga ggatataggg cagcagcgtg gcagcggcgc tctcggcagc
     1261 atacgccggc ttcggcaaca gcccgagcgc ccccgccatg ccgagccaaa ccgtcacccg
     1321 caccgatcaa cccttcgacc cgttgccgtg gttcttgcgc gccgcggtcg cccgcgcgcc
     1381 cgtcgtagcc gattatggcg gtggcccatg aattttcaca gggtgaaaaa ccgcggaccg
     1441 gtcagtagcg atagcgttcg ggcttgaacg gccccttctc gccgacgccg agataagcgg
     1501 cctgcttggc gtcgagcttg gtgagcttga cgccgagctt ctcgagatgg agcgccgcga
     1561 ccttttcgtc gagctgcttc ggcagcacat agaccttgtt ctcgtagcgg ccctgattgg
     1621 tgtagagctc gatctgcgcc agcacctgat tggtgaagga ggcgctcatc acgaagctcg
     1681 gatggccggt cgcgcagccg agattgacca gccgtccctt tgccaggacg atgaggcgct
     1741 tgccgtcggg gaacacgacc tcatcgacct gcggcttcac ttcctcccag cgatagttgt
     1801 gcagcgccgc gatctggatc tcgctgtcga aatggccgat gttgcagacg atggcgcgat
     1861 ccttcatccg ccgcatatgg tcgagcgtga tgacgtcgat attgccggtc gcggtgacga
     1921 agatgtcgcc gatcggcgcg acgtcttcca tggtgagcac ctgatagccc tccatgcgcg
     1981 cctgcagcgc gcagatcgga tcgatctcgg tgacgatgac ctgggcgccc tggctgcgca
     2041 agctctctgc cgagcccttg ccgacatcgc catagccgca gaccacggcc accttgccgg
     2101 cgatcatcac gtcggtcgcg cgcttgatgc cgtcgacgag gctctcgcgc acgccgtaga
     2161 gattgtcgaa tttcgacttg gtgacgctgt cattgacgtt gaaagccggg accttgagcg
     2221 tgcccttctt catcatctcg tagaggcggt ggacgccggt ggtcgtctcc tccgagatgc
     2281 cgcgaatgtc gttcagcagc tcgggatgct tctcgtgcac gatcttggtc gcgtcgccgc
     2341 cgtcgtcgag caccatgttc ggcttccagt cattgggacc gcgcagcgtc tgctcgatgc
     2401 accactcata ctcctcctct gtctcgccct tccaggcgaa gaccgggacg ccgcgcgccg
     2461 cgatcgccgc agcggcctga tcctgggtcg agaagatgtt gcatgagctc cagcgcaccg
     2521 ttgcgccaag ctccatcagc gtctcgatca gcaccgcagt ctgaatggtc atgtggaggc
     2581 agccggtgat ccgagccccc ttcaagggct tcttcgcgcc atattcgcgg cgcagcgcca
     2641 tgaggcccgg catctccgtc tcggcgatcg cgatctcgcg cctgccccag tcggcaaggg
     2701 cgatgtcggc gaccttgtag tcggcggaag cggccatggc gtgctcctcg aagggatgga
     2761 agcgccgcat atagcagcgc cgtccggcga cggcaagact cgcgagacgc gcgacgggtt
     2821 cgctgcgtcg cgacgacccg tcgctcaggg caaggttgcg aggagggcct gcgccgtcct
     2881 gtgatcgatg gaatcgcggc cttcggtgaa gatctcgtag atcggccgca gcatctcacc
     2941 cgccgcgccc tcgcggcctt gcgcgcgcca gagacgcgca aggctcgtcg ccgtccgcaa
     3001 ctgccaggat cgcgctccct gccggccggc cagcgccatc gagtcgtgaa aatccctctc
     3061 cgcggcaagc gacgcatcgg gcagcgcgca gcgtaacagg agctcgcctc tgacgcgcag
     3121 caactcgggc aagcaccagc ctgcctcgtc acgcgacgcc tcggcgagcg cctgctcgat
     3181 cgtggcgagg ccctccgccg cgcgcccgac cgcgctcaac gcctcggcca tcgcgccaac
     3241 gaagatcggc cggtgcagac cgaggcggcc ttgcttgaag tcggcaagcg cgcgcgtcat
     3301 cagttcgagg ccgacgagcg catcgccgcg cttgatggcc atccaccctt tcaaggccat
     3361 gctgtcgact tcccacatgc cgaggccgtg cttttcggcc ccgtcggtga gaagcggcgc
     3421 gaattcctcc accgcccgcg gctcgccgga catggtggcg agaccgcagg cgccgaagca
     3481 gagcgcgacg cagaaggagt tcacgtgatt gacgatgcgc gcctcctcga ccgccgcctg
     3541 cgcgacgcgg gccgcctggt cggggaagcc ttggagccac aggatgcgcg ccaggcacga
     3601 taaggcggag acgcgctgat cgacgccgaa acggagcacg taggaacgac ggacgctggc
     3661 atcctgatcc gcgatgacgg tctcgagcgc gcgccgcgcc cgcgcgtatt ccgcgaggtt
     3721 gaagagcgac acgccttcca tgcgaccgcc ggtccgcacc gccgcgacat cgccctccgc
     3781 ctcggcgacc tggcggaatc gttcggcgat cgcgagcgac atccgatggg cgccgattcg
     3841 caattggcag atccacagat tgtagagcgc cgtcagctgg tattcggcat cgcccagacg
     3901 ctcggcgaga tcgagtgccc gcgtattcgc cgatcccgcc tcgccgaccg ccgccttggc
     3961 ccacatcgac gagcggccga gcgccgcctg cagccgcatc tcgtgccggc tctggcggtt
     4021 cgcgcccttg gccaggtggc gaagcgcccg atcgacgagg tcccggcact cgccgagtat
     4081 cgacagcagg gtccagagcg gcaccgccgc ggcggcgagc gccacgccga tctccgcatc
     4141 cccatcgctg tcgaacgccc attcgagcgc ggcgcggatg ttgtcgactt cgggcgcgaa
     4201 tttggcgatc aactccgatg ctggcgtggt ctgccaagcc gcctccgccg cctcgagcag
     4261 cgcgcgcaga tgatcggcgt ggccgcgcgc cgccatggcg aattcaccgc tctcggcaag
     4321 cttcgccaga gcgtaggcgc gcgtcgtgtc gagcagacgg tatcgcgcga cggagcggcg
     4381 gacatcgaag gtcaccagcg atttctgcac gagccgcatg accatgtcgg aagcctgggc
     4441 gggatcgaga ccatcgcccg cagcgaccac cccggcgctg tcgagcgtga agctgcccgc
     4501 gaaaatcgcg atgcgccgca gcacggtgcg ctccggcgcg ctcagcagct catagcccca
     4561 gtcgagagca gcgctgagcg tctggtgccg ccacatcgcc gtgcggcgtc cgtccgtcag
     4621 cagctggaac cggtcgtgga gccggcgcgc gagcatgtcg acgccgatgg tcgctacccg
     4681 tgccgccgcc aactcgatcg ccagcggaat accgtcgagc cggcgacaga tcttgctcac
     4741 acccggcatc gtgcaatcgt cgagcacaaa gagcggattg gccgcctgcg cccgctcggc
     4801 gaagagctgg acggcactgt gctcgatagc ggtcgcgaca ttgctggtat ccgtcgcggg
     4861 aaaggtcagc ggacgcacgg gatagagatg ctcgccttcg gccacgagcg gctcgcgact
     4921 ggttgtcagc acatgcagtt gcggcgcgcc tctgagcagc agctccgcga gctgcgcgac
     4981 cgcgccgatc acatgctcgc agttgtcgat caccagcagc aggtgcttcg gcgcgagcgc
     5041 cgcgatgatc tgatcgacga ggttgtgatt ggatcgcgag gggacgccga cgacgcgggc
     5101 gatcgcatgc ggcacgagct cggcgtcctt gagcgcgtcg agctcaacca tccaggcgcc
     5161 gtcggcaaag tgcggcaaag ccagccgcgc cgcgcgcagc gccagctgag tcttgccgat
     5221 gccccctggc ccgatgagcg tgacgagccg ctgcgtcgtg actcgataga gcactgcctt
     5281 gaggtcatgc tcgcggccta ccagcgacga gatcggcgtc ggcagattgg tcgtcgcctc
     5341 agtcagcgcc ttgacatggg atagtttcgg tatcgcttcc gccgcattcg cgatcttgcg
     5401 cacagcggcg gtgaagcgat agccgcgccc ggcatcggtc ttgacgagat cccggccatt
     5461 gcccagggcc ttgcgcacgg ccgagacctg caccgtgacg ttggtcttgt cgacgaccat
     5521 gccgggccag acgcggcgca gcagttcgtc gttgctgacg acccggccct ccgcttcgac
     5581 gagcacgagc agagtgtcga aggctctgga gccgagctcg acaggcgtcg agcccagcag
     5641 caactggcgc cgccggggca agaggaggtg ctctccgaag gagagcgcga tctcgtcggg
     5701 gtcgatcgcc agctcaatct cgctcgcgcc ggggaccgct tgggagtcct cagtcagctg
     5761 catgcttgct cctgagcgga gcagccccca ccaagtcccg cgaccgcgat actcgccggc
     5821 cgagggccgt ccgcgaccag attactgcac aagttatagg catgaccgcg ggctcatcaa
     5881 ggtctctttg tgccgactgc tcaatcttta tacgcagcga gggcctccgt cgttacgaac
     5941 aattcgcatc atttgaagca gtttgagaat gtgctgccct acgctttggc cgccgcgggg
     6001 aatgttggca ccgagtcggg gtacaaggat gttggacgtc gcccatctcc gcttcagcga
     6061 atcgcgcgct cggtcacggg ccacagagtc cccgcctttg ccgccacccg ttcctgacga
     6121 gtcgatgcaa gcggtcctcg gccgccggtt gcggtggctg cgcgtcgagc gcggcttcag
     6181 ccgcaagacg gtggccgctc gcctcggcct gccggtgacg ctggttgaac gacacgagcg
     6241 aggaatggcc cggctcgagg ctcaacaact cgcggcctac gcccgatttt actgtatccg
     6301 catcagcttc ctcttccgcg acccgccggc agcggctgcg ggcgcctgac gctccatctg
     6361 tcgcgcccgc ggctcggagt ccccggcgtc acgccagcgc gttgaagtcg tcaagcaggc
     6421 tggcaatgcg gtcatcatcg agctggtcca tgatggcgcg ggcgcgccgg cccgccgcca
     6481 ccatgtcgag cgtacggatg cgccgcttga cgaggccgat cttggcgggc gccatcgaga
     6541 ggtcgcggat gccgagcccc aaaagcagag ccgtgtagcg cggatcgccg gccatctcgc
     6601 cgcagaggct caccggaagg cgcgcgcgga gcgccgcttc cacggcgaac tggatgaggc
     6661 gcaacaccgc cggatgcagc ggattataga ggtgcgccac ctgctcgtcg ccgcggtcga
     6721 tggcgagcgt gtattggatg agatcgttgg tgccgatggc gaagaaatcg cacgccgcgg
     6781 cgagcgcatc ggcggcaagc gccgcgcccg ggatctcgat catcaccccc aagggcggca
     6841 gcggatcggc gatcttgagg ccgcgccgct tcaatcgccg cacgacctgt cccaacgcct
     6901 cccgcacttt tagaacctcg gcgacgctgc acaccatcgg caggaggatg cgcaccggcc
     6961 catcggcgcc ggcgcgcagg atggcggcga gctgggcgtc gaggagcggc cgctccttga
     7021 gcgaaaggcg gatggcgcgc aggcccaacg ccggattggc gcccacgcct tcgagcgcct
     7081 cggcgagcgg cgccgccagc ttgtcgccgc cgatgtcgag cgtgcggatc gtcacggacc
     7141 ggccgtccat gccgcgcacg agcccgcgat aagcttcgta ttgctcgtcc tcgtccggca
     7201 ggtcttcgcg gttcatgtag aggaactcgg tgcgcaccag cccgatcccg gcggcaccgg
     7261 ccgcgatcgc ctgctcgagt tcgcgcggca gctcgagatt ggcctggagc acgatctcga
     7321 cgtcgtcgcg cgttaccgcc ggcagacgct tcaaacgcgc gagctgtcgg cgctcgagcg
     7381 ccagcacctc gcgctgcgcc tcgaaggaga cgacggtctc gcgtgtcgga ttgatcacga
     7441 ccgttccact ggccccgtcg atcaacacca tgtcgcccgc gtcgatctgg ccgaggagac
     7501 cggtcacgcc gagcaccgcc ggcaagccca gagcccgggc catgatggcg gtatggctct
     7561 cggcgccgcc cagcaccgcg gcaaaggccg cgatgcgctg cgggtccatc agtgccgtgt
     7621 cggcgggcgt cagctcctcc gccaggatca ccgtcccttc cggcacatgc tggagtgcgg
     7681 catagggcgt cttggtgagg ttgcggatga gccgcgcgcc gacgacgcgg atgtcatcga
     7741 cgcgcgccgc aagataggcg tcgcccatcg cggcgaagct ctcgctgatc tgcgcgatct
     7801 ccatctgcac ggcgcgctcg gcgttaatac gctcctcgcg gattcgccgc tcgacgccgc
     7861 gcacaaggcg cgacttcgtc agcatggcga tatgcgcatc gaggagatag cccacttcct
     7921 cggctgccga gtccggcagt gccgaggact tgcccttgag cttccgcagc tgcttcagcg
     7981 atagcgagag cgccgcggcg aagcgctcgc cctcggcttc gacctcgccg gcggcaatcc
     8041 ggcgctcggg cacggcgata tcgccatgat cgctgacgaa ggcggggccg agcgcgatgc
     8101 cgggcgagac gcccagacca tgcagcaccc gctcgcgctt gccgggcttg tccccggcaa
     8161 agggcggcgg ctcatttttc atcgaagccg ccggcgatca gctcggcaag cgcgccgatc
     8221 gcgcgctcgg cctcggggcc gctggcgcgc agctcgatgt cggtgccggg accggcggcg
     8281 agcatcatca gccccatgat cgagcggccc gagacggcgg cgccgttctt ggccacgaaa
     8341 acctcggcgt cgaactgcca ggcaagcttg acgaagcgcg ccgccgcccg cgcatgaagg
     8401 ccccgcttgt tgcagatcgt gacggtgcgc cggatcggcg ccgccccatc ggcggccgac
     8461 ggctcgccgg tatcgttcat ccgcgaacgt cggcgaggag ctgcgaggcg acgttgatgt
     8521 atttgcgccc cgcctcttgc gcgccgcgca ccgcgttggc gagcgtctcg gtcgggcgca
     8581 aactcgcgag cttgatcagc atgggcagat tgatgccggc gatgacttcg atcttcgcct
     8641 tgtccatcac tgaaatggcg agattggagg gagtgccgcc gaacatgtcg gtgagcagca
     8701 ccacgccctg cccggcatcg acttcctcga cgcagcgcag gatgtcctgc cgccgcttct
     8761 ccatgtcgtc gtcgggaccg atgcatacgg cggcgatgtt gcgttgcggc ccgaccacat
     8821 gctcgagcgc ggcgacgaac tccgcggcca gccggccatg ggtgacaagg accattccga
     8881 tcatgcgata tccttcacgg gctttggcgc cgcggcgttc gtgccggtgg ccagcaacgt
     8941 cgccgactgg tcgagatcgc ggtgattgac gccgacgggt ctgccttgtt cgccgagcca
     9001 ttccgccacc cgctcggcga gataaacaga gcgatggcgg ccgccggtgc agccgaaggc
     9061 gatggtgaga tagctctttc cctcgcgctc gaagcgcggc agcagcggcg cgagccactg
     9121 gcagagccgc tcgaagaatg gggcgaagtc cgcatcggcg gcgatgaagg cggccacctc
     9181 cgggtcgcgc ccggtgagcg gacgcagcgc cgccacataa tgcgggttgc gcaggaagcg
     9241 cgcgtcgaac acgaggtcag cgtcgcgcgg caggccgtgg cgataggaga aggaggtcac
     9301 gaagatggcg atgccgggcg ctgcattgag cgcgaagtga ccatgaagga cgcgcttcaa
     9361 gtcgctcgga ttgagggcgg tcgtgtcgat caccaggtcg gcgcgatccc tgagcggcga
     9421 gacgcgttgc cgctccagca ggatgccgtc gatcaccggc ctgtcgccgg ccagcggatg
     9481 gcgccgccgc gtttccgtgt agcggcgcac gagccagtcg tcctcgcagt cgaggaagac
     9541 gatctgcagc tcgagatgat gctcggcgac cacgcggtcg agcgccgcga ccagcggccc
     9601 gacgccgaaa tcccgcgtgc gcacgtcgat gccgatggcg agcggcggcc gctcggccgg
     9661 catctcgagg agactcggca gcagcgacag cggcaggttg tcgaccgcct cgtagcccag
     9721 atcctcgagc gccttgagtg cggtggagcg gccggcgccg gacatgccgg tgacgaccag
     9781 aagccggctc gctgccgggg tcgaggcttc aaacttcggc gccagcttgt cgctcatgag
     9841 gtgagcggca atgacgcccg cgccagcacc ggcagcgcgc ggagcgccag gcgcagcttg
     9901 gcagcggccg aagcttcgaa gggcgccagc cggaggcgcg gcacgcggca gcccaggaac
     9961 tcgcaggtca ggggctcggg aagacgctcc acggcgtcgt tcgcgaccag atcgacgacg
    10021 aggcgcagcg gcgcgctttt gacgctgtcg acacgcagga tgccgacgcc ccggacttcg
    10081 atttggccgg cgatcgtcga cggcgcgctc aggcgcaacg ccccgccgat gaatcgcagt
    10141 tcggtctggt cgtcggcgac gagccgcgcc ccctggtcga tgaggcgcaa ggcgagatcg
    10201 gacttgccgc tgcccgaggg cccccgcaac agaacgccgt cgccgccaag gacaacggaa
    10261 gtgccatgga cgcggatcat gaggcgatct tcgtgcgccg cccgcacgcc aaagtcaatg
    10321 gctgcgggtc atgcccaatg gtctgcgtcg cgggcaatac aagatttagc tggcggcgat
    10381 ctccaggcca cgatatctgg gtattctgcc gtgaaacctg cagggacggc atatgctcta
    10441 acaaataatt gatgcccaca gacttgcaag tgcaggttgt agatcgtaga taggagtttc
    10501 gggcgatttc agctctctat gacgacaaaa actgctgcca gtttgtctcg acatgggagg
    10561 agacgatgca tgcaacgacg acgtccaccg atcccgaggc ccaagatcgc acgtcgcggc
    10621 tgcgaaagca gatcttgaag ctgcgctgga taggcatgga gagcgaggcc gccgagctcg
    10681 ctcgttccgc cgcctgtccc gacattctgc tccgagaaat cgccgagcgg gactgacgca
    10741 ataccaaagc ccgccggtca ccccggcaac cgcacgatga agcacgcgcc gatgacgctg
    10801 ccgtcgacgt cgcggcggtt ctcggcgcgg atcgtgccgc gatgcgcctc gacgatctgc
    10861 ttcgagatcg agaggccaag gcccgaatgc gtcccgaatt tctcgccggc ggggcgctcg
    10921 gtgtagaagc gctcgaagat cgcctcgagc ttgccgtcgg gcatgccggg tccttcatcc
    10981 tcgatggtga cgaccaccgc gtcgccgtcg cgctttgccg caatgtgaat cgcggcagcc
    11041 ggcgggctga aggagatggc gttggcgatg aggttgcgga agacctggac gagccggccc
    11101 tcgatgccga gcacgcgcag atcgccgtcg ggcgtgccca ccggccccgg cagcgccagc
    11161 accagccgct gtgccttctc gtccgacgtc gactgatgca catcgaccag cgtctcgagc
    11221 atcggtccca ccagcaccgg ctcgagttcc acccgcgaca gctcggcgtc gagccgcgac
    11281 gcgtcggaga tgtcggtgat gaggcggtcg aggcgctgca catcgtcgag cacgagcgag
    11341 agcagcttcc gctgcttctg cggatcatcg acgcgcgccg cggtctcgac cgcgctcctg
    11401 agcgacgtga gcggattctt gatctcatgc gcgacatcgg cagcgaaatt ctcgatcgca
    11461 tccatgcgtt gccagagcgc gctcgtcatc tcgcgcagcg cgcccgagag gtcgccgatc
    11521 tcgtcgccgc gccgcgtgaa atcggggatc ggcacttgcc gcccgtggcc gcggcgcacg
    11581 cgctccgcgg cggcggcgag gcggcgaatg ggacgcgcaa tcgtgccggc gagatagatc
    11641 gacagcagca ccgtgacgct gagcgcgacg gcaaaccatt tgaggatctc gaaccgcgcc
    11701 gcgcgcaagg tctggtcgat ctcgctgctg tccgtcgaca acatgaccgc gcccagcact
    11761 tgcttgtagc gctgcacggg aacggcgacg ctgagcacga ggccgctgct gtcgcggtcg
    11821 cggcgcacgg cgttctccac ctcgcccaga agcgcctgct gcacttcgcg atagtcgccg
    11881 gcgctctgcg ccgcggcttc ttcgtagggc ggataatgct tttgccccgg cagaaggccg
    11941 agcagggcgt cgtagagcca ttcgctcagg cgatcgagcg cgccgcgctc gcccggcggc
    12001 ggcagctcgt tgatctgcac catcccgccg ggaccgcgca gcacgcggct gtcggcgatg
    12061 agcttgccct gggcgtcgaa gagccgcgcg cgggtatggg tgggctcgac cagccgccgc
    12121 atcatctggc ggccgagctc ggggatgagc gtctcgcctt cgccgagcga atcgatgacg
    12181 gcgccctcgc ccagcgccgc ggcgaagatc tggccctggg tgcgcaaagc gtcgagctcg
    12241 gccgcgatca ggctgcgctg gtattcgccg agatagagca gtccgaagac gaggaggccg
    12301 agcgccagga cattgacggt cagaatctgg cgggtgagcg gcgagaggcg gggccgccgc
    12361 gggcgatcgg gggcgaacgc cggcgcgggc ggggcgacgg cgggcggttg cgggcgcggc
    12421 tcagcggtcg cggtagcgat atccgacccc atagagggtt tcgatctggg cgaagctctg
    12481 gtcgatgacc ttgaacttct tgcgcagccg cttgatgtgg ctgtcgatcg tgcggtcgtc
    12541 gacatagatg tgctcgccgt aagcgccgtc catgagctgg tcgcggctct tgacatgccc
    12601 cgggcgcagc gcgagcgact tcaggatcag gaactcggtc acggtgagat cgacattgtg
    12661 gccctgccag ctgcattgat ggcgccccgg gtcgagcacg agctcgcccc gcgtgaccac
    12721 gggctcgctc tcgcccttgc cgcgcgccag gtcgccgcgg cgcaagagcg cgcggatgcg
    12781 ctcgatgagc agccgttgcg agaacggctt cttgatgtag tcgtcggcgc ccatgcgcag
    12841 gcccagcacc tcgtccacct cgtcatcctt cgaggtgagg aagatcaccg gcatgttccc
    12901 ctgcttgcgc agatggccca gcacctccat gccgtccatg cgcggcatct tgatgtcgag
    12961 gatcgcgagg tcggcgggat gcgcggcgag gccccggagc gcctcggcgc cgtccgagta
    13021 tgtccgcacc tggaagccct cggcctcgag cgcgatggcg accgaggtga ggatgttctt
    13081 gtcgtcatcg acgagagcga tggtattggc cacgagcctg agccctttcc cgcgaaatga
    13141 gcgcgcggat actcgccctc cagtgtggcg cctttaaggc gctcccgcgg agccgatcgg
    13201 gcgagattag cgccgccggg ccgcggattc catggtgcgg aacggctctt tccttgttgc
    13261 cgggacatgg cgtggcgtaa ggttttccgg caccgaactt gcagagcgtc accatcgcac
    13321 agacgggcga caaggccagc cccaggggag atcggcgatg aaaagcttca tgaggggtct
    13381 gcgccgggcg gcgatgctcg cgctcggggc agcgatgatc gtgccccagg cccacgccca
    13441 atctgcggcg aagaccatca agttcattcc cgaagcggat ctgcgcagcc tcgatccgat
    13501 ctggaccacc gcctacatca cgcgcaatca cggctacatg gtctatgaca cgctcttcgc
    13561 gctcgatgag catctccagc cgcggccgca gatggtcgac cgctggacgg cgagcgagga
    13621 tcggctcact tacaccttca cgctccgcga cggcctcaaa ttccatgacg gcgcgccggt
    13681 gcgctcggcc gactgcattg cctcgctgca gcgctggatg aagcgcgacg gcctcggcca
    13741 ggtgctgggc gatgccatgg cggaaatgcc cgtgacggac gacaaaacct tcgcgatcgt
    13801 gctgaagcgg ccgttcccgc tgctgctcga agcgctcggc aagctctcga gcaatgttcc
    13861 cttcatcatg ccggagcgca tcgccaggac cgatcccaac acccagatca ccgatccgac
    13921 cggctcgggc cccttcaaat tcgtcaagga ggaatggatc cccggcaaca aggcggtcta
    13981 tgtgaagaac accgactacg tgccgcgcgc cgagccgccg agctgggccg cgggcggcaa
    14041 ggtggtcaag gtcgaccgcg tcgaatggct ctacattccc gacagcgcga ccgccgccgc
    14101 cgcgctcaat gccggcgagg tcgattggtg gcaacagctc ccgcccgatc tcgtgccgct
    14161 gctcgagcgc aacaaggacg tcaccgtcgc caatgtcgat cccctcggca gcatcggcgt
    14221 catgcgcttc aaccagctgc agccgccctt cgacaacgcg gctctgcgcc gcgccgtgct
    14281 ctatgccatc gaccagcagg acatcatggc ggcgctcggc ggcgatgcca agaactggaa
    14341 gacctgctat tcggtctata cttgcggcac gccgctcgcg agcgagaccg gcgccgacgc
    14401 gctcaagggc aagcgcgacc tcgacaaggc gaagcagctc gtcaaggaga gcggctacaa
    14461 gggcgagcgc gtcgtcgtga tgaccgcgac cgaccagccg atcgtcaacg cccaggccaa
    14521 tgtcatcgcc gaggagttgc gccagatcgg cctcaatgtc gaccttcagg ccatggactg
    14581 gggcacgctc atcacgcggc gggcgagccg cgagccggtc gacaagaatg gctggagcat
    14641 cttctgcacc tggctcgtcg gtcccgacat gatcaacccg gcgctcagtt tcccgctgcg
    14701 cgccaatggc gcgaaagcct ggttcggctg gccgaccgac gacaagatcg aggcgctgcg
    14761 cgcgcaatgg atcgaggctc ccgatctcgg gacccagcag aagctcgcgg ccgcgcttca
    14821 ggaagaagcg ttcacgagcg tgcccttcgt gcccacgggc cagttcgtca tccccaccgc
    14881 cttccgcaag agtctgaaag gcgtcgtgcc ggcgccggtg gtgttcctgt ggaatgtgga
    14941 gaagagttag tggcggctgc tgcagtacgg ccgtgcgtcc ttcgacaggc tcaggatgag
    15001 gaagagccgg gacggcatcg actgtcgatc cgagaaagaa aaatcctcat cctgagcttg
    15061 tcgaaggacg caccgacagt attgcagcac accgccggcc acacgcgcgt gccctaacga
    15121 tgctcgccta catcctccgc cgtcttgccg cgacggtgcc cgtgatgggc gtggtcgccg
    15181 tcgcggtgtt ctcgctcctc tatatcgcgc cgggcgaccc cgccgcgatg atcgccggcg
    15241 acctcgcgac ggccgacgac atcgcgcgga tccaccagca gctcggcctc gacaagccct
    15301 tcgtcatccg cttcggcggc tggctctggg gcgtgctgca tggcgatctc ggcacgtcga
    15361 tcgtatcgag ccagccggtc gcggtgctca tcggtcagcg catcgaagcg acggcggcgc
    15421 tcaccgtgat gacgctgctc ctcagcatcg ccttcgccat ccctttgggc gtgctcgccg
    15481 cctggcgggt cggcacctgg atcgaccgca gcgtgatggt gctcgccgtg ctcggctttt
    15541 ccgtgccgtc cttcgtgctg gcctatctgc tgatcctcgg attctcggtc gagctcaatc
    15601 ttctgccggt ccagggctat gtcagcatcc gcgaaggctt cgctccgttc atcgcccatc
    15661 tcatcctgcc cagcatcgcg ctcggactga tctatggcgc gctcatcgcc cgcatcacgc
    15721 gcgccagcat gctcgaggtg ctcagtcagg attacatccg cacggcccgt gccaaggggc
    15781 tggccgacaa cgtcgtgctc cgccgccatg cgctgcggaa cgcggcaatt cccatcgtca
    15841 ccgtcatcgg catcggtttg gggctcctca tcagcggcgt ggtcgtcacc gagaccgtgt
    15901 tcgccatccc cggcatcggc cggctcaccg tcgacgcgat cctgcgccgc gactatccgg
    15961 tgatccaggg cgtcatcctg ctgttctcct tcgcttatgt gctggtcaat ctcgcgatcg
    16021 acatcagcta cacactgctc gacccccgcg tgaggtattg agatggcagt gatgcgggcc
    16081 gagctgctgg cgcccgcctc cgggcggcat tggctcggcg atgccgtgcg ccgccatccc
    16141 acggtcgcgg ccggcgggtt gctgctgctg gccatggcgg cggcggcaat cctcgcgccc
    16201 tggctcggca cggtcgatcc gctgcatggc gaggtggtga agcggctgcg ctttccctcg
    16261 gcgcagtcct ggttcggcac cgatgccttc ggccgcgacg tctacagccg caccgtcttt
    16321 ggtgcccgcg tctcgctcga gatcgggctc ggcgtcgccc tcttcagcac ggcgctcggc
    16381 ctcgtcatcg gcctcgtcac cggcttcagc cgcatcgccg atgccatcgt catgcgcatc
    16441 atggacgggc tcatgtcgat cccgccggta ctgctcgcga tcgctctcat ggcgctcacc
    16501 catgcgagta tcggcaacgt gatgatcgcc atcacggtcg ccgaagtgcc gcgtgtcacg
    16561 cggctcgtgc gcggcgtcgt cttgaccctg cgcgagcagc ccttcgtcga agcggcggtc
    16621 gcggcgggca gcggcctctt gcgcatcttg cgccgccaca tcctgcccaa cacgctcgca
    16681 ccggttctgg tgcaggcgac ctatatctgc gcctcggcca tgatcaccga agcggtgctg
    16741 agcttcatcg gcgccggcac gccgccggag attccaagct ggggcaacat catggccgag
    16801 ggccggagct tctttcagat ccgcccctac ctcgtgctct ttcccggcgt gttcctgtct
    16861 ctgacggtgt tggcggtgaa cctcgtcggc gacggcctgc gcgacgccct cgacccgcgc
    16921 ctcgcgcgaa gactctagtg tcgacagtgt cgcctgcggt caccccccac cctgaccctc
    16981 cccctcaagg ggggagggga atacaaagct cgcacggatc ttccccctcc ccccttgagg
    17041 gggagggagg ggtggggggt gacgccgccc atatctcgcc gctcctcgag gtcagcgatc
    17101 tccgaacgca tttcttcacc cgcgacggcg tcgtccgcgc ggtcgacggc gtctccttcg
    17161 ccgtcaacgc cggggagact cttgcggtcg tgggcgaatc cggctgcggc aagagcgtca
    17221 ccgcgctgtc gatcctgcgc ctcgtccccg acccgcccgg ccgcatcgtc ggcggggcgc
    17281 tgcgctttgc cggccgcgac ttgcttcgcc tgaccgagag cgcgatgcgc gacgtccgcg
    17341 gcaacgagat ctcgatggtg ttccaggagc cgatgacctc gctcaatccg gtgctcacct
    17401 tgggccgcca gatcggcgag acgctgatgc gccatcaagg cctcgacgcg cgggccgcca
    17461 atgcgcgcgc catcgacatg ctgcgcctcg tgcaaatccc cgagcccgag cggcgcgcgc
    17521 ggcaatatcc gcacgagctg tcgggcggca tgcgccagcg cgtcatgatt gcgatcgcgc
    17581 tggcctgcaa tccgaagctg ctcatcgccg acgagcccac cacggcgctc gacgtcacga
    17641 tccaggcaca gatcctcgat ctcatgcgcg cgctcaaggc acggatcggg gccgcgatca
    17701 tcctcatcac tcatgatctg ggcgtggtcg ccgagatggc cgagcgcgtc gtcgtcatgt
    17761 atgcgggacg gaaggtcgag gaagcagcgg tgcgcgcgct tttccgccgg ccgcgtcatc
    17821 cctatacgct cgggctcctg gccgcggtgc cgcgcctcgg cgcgacgctc ggccgcgagc
    17881 cggcgccgct cgccgagatc cccggcgtgg tgccgtcctt gcgcgagaag atcatcggct
    17941 gcgccttcgc accgcgctgc gcttatgtca cgccgcgctg ccgcagcgag gcgccgtcgc
    18001 tcgaggccaa agcgccggca catctcgccg cctgcttcga aagcgagcgg ctgccgtcat
    18061 gagcgcgcag agcacgccgc tcctcgaggt ggtcggcctc gtgaagcatt ttcccgtgcg
    18121 ccgcggcctc ttctcgcgcc gcacaggcgc tgtccgcgca gtcgacggca tcagcttcgc
    18181 catcgccgcc ggcgagacgc tcggcctcgt cggcgaatcc ggctgcggca aatcgacggc
    18241 ggcaaagacc atcctgaagc tgatcgagcc gacggccggc accgtgcggc tgcgcggcga
    18301 gcgcatcgat catctgccgc cccgtgccat gcgacattgg cgccgcgagc tgcaggtggt
    18361 gttccaggat ccctattcct cgctcaatcc gcgccttcgc gccggcgaca tcgtcgccga
    18421 gccgctcgtc aatttcggcg tcatgcgcgg cgccgcgctg cgcgaccgcg tcgccggcct
    18481 tttcgctcaa gtggggctca gcgccgatgc gatgcggcgc tatccgcacg aattctcggg
    18541 cggccagcgc cagcgcatcg gtatcgcccg cgccctcgcg ctcgatccga gcctcgtcgt
    18601 ctgcgacgag cccgtctcgg cgctcgacgt ctccgtgcag gcgcaggtga tcaacctctt
    18661 gaaggagctg cagcgcgagc ggcacctctc ctatctcttc gtcgcgcacg acctcgcggt
    18721 ggtcgagcat atcagccacc gcgtcgccgt gatgtatctc ggcaagatcg tcgagctcgc
    18781 gcccaaggac gccctcttta gcgcgccgct ccacccctat accgaggctt tgctctcggc
    18841 agtgccgcaa cccgaccccg aaacaaaacg caaccgtatc atcctcaggg gcgacgtgcc
    18901 gagcccgatc gatccgccgc ccggctgccg cttccacacg cgctgtctct atgcggagcc
    18961 gcgctgccgt atcgacgagc cggagctgcg cgaagtcgca ccgggccacc gcgtcgcgtg
    19021 ccatttgcgg ccggtcgcgg cgatgtagtc gcactgccga tcggctcgct atgggattgg
    19081 ctcaggccgt accgcccgtc tcctgccgca ggcgctggcg ccgcagcgcg cggcccgggc
    19141 gagcacccgt cgccgcgccg tcgctccaca ccgccacgcc attcaccagc accagatgga
    19201 tgccggccgc tggggtggtc ggcgcctcga agctcgctcg atcgatgatg gtggcggggt
    19261 cgaacacggt gatgtcggca taggcccccg gcacgacatg gccgcgcccg gaaagaccga
    19321 agcgcgcggc tgggagcccg gtcatgcgcc gcacggcctc ttcgagcggg aacagtttcg
    19381 cgtcgcggca ataatggccg agcaaccgcg cgaaagtgcc ccagaggcgc ggatgcggat
    19441 ggcgatcatg cggcaggccg tcggagccga tcatggtgtg cgggaaggcg agcacgcggc
    19501 gcacatcctc ctcgctcatc atgaaataga tggccccggc gggctggagc cgcgcggcgg
    19561 cttcggcctc gctcgcgccc caatcgcgcg cgatgtcggc gagtgtgcgt cccgaggcct
    19621 tgggatgcgg cttcgaccag gtgatgagga tcttggcggc gcggggaatg ttctcggcca
    19681 gcagcacggt cgaggaggca atgtaaggat aggcgtcgag agccagcggc tgctccgcca
    19741 tcgctgcggc gatccgcggc agggtctccc gcgtgcggcc gaaattctgc gttcccagca
    19801 ccttgtgatg cgagatcacc accgggatcc cggccgcgcg cccgaccgcg aagctctcct
    19861 cgaggctgtc gagcacatga tcgccttcat cgcgcatatg ggtcgtatag atcgcgccgg
    19921 cgtcgcgcag cgtctgcgcc agcgccacca cctccgcttc gggcgcggct ctcgcgggcg
    19981 cataggcaag gccgctcgaa agcccgatgg cgccggcctc gagcgcctcg gccagatgct
    20041 cgcgcatcgc cgcgatctcg ctttcgctcg cgggccggtc gagccggtcc atgacgcaag
    20101 cgcgcaaggt cgagtgaccg atgaggctgg cggcgttggc ggcagccggc gcctggtcga
    20161 gcgccgccag atagtcaccg aagcggcgga agcggaaatg ctcggcgtcg ccgatgagat
    20221 cgagcggcgg cggcagcggt cggccggcca gcatcggcgc gaggctcacg ccgcaattgc
    20281 cgaccaccac cgtggtgacg ccctggctcg ccttgggcgc catgtcgccg cgggacagca
    20341 gcgcgcggtc gtcatgggtg tgcacgtcga tgaagcccgg cgcgaccgca agaccatcga
    20401 catcgacctc cgcggcgccg ctcgccggcg gcaggccctc cacggcgacg atgcggtcac
    20461 cgacgatggc gacatcggcc gtgatcgccg gcgccccggt gccgtcgatc acgctcccgc
    20521 cgcgcagcac aagatcgtag cgcgccatgg cgcgaggcta tgcgatcgct ccggcgatgg
    20581 aaagtatgcg ccgacttggc cctcgcccgg caccgcgaaa gggtgagggc cggcgacgcc
    20641 aagagatcac gcacaggtgg attgccctct cccgccgcct gggttagatc aattctacgt
    20701 tatggcgaca gaacgcgcgg atactccggc ggcgacggcg cggcgactga tgcgcgcgct
    20761 cgaccgcgcc accctcgcta ccagcctcga aggctggccc tatgcttctt tggtgctggt
    20821 ggcgctggat cacgacgcga gcccgctcct gctcctctcc gaccttgccg agcacagcaa
    20881 gaacctcgcc cgcgatcggc gcgcctcgct gctcttcgac ggcaccgccg ggcgcgacga
    20941 ccccctcacc ggggcgcggg tgacagtgct cggcgagatc cagccgatcg cggatgagcg
    21001 cctgctgtcg cgcttcactt cgcggcatcc ctcggcggcg ggatatgccg gcttcgccga
    21061 tttccgcctc taccggctca ccgtcgcccg cgcccatctc gtcgccggct tcggccggat
    21121 ccactggatc gccgccgacg ccctcctctc cccggccgcc cccgcgcttg ccgaggccga
    21181 gcccggcatc ctcgagcaca tgaacaagga ccacggcgag gtcatcgacc tctacgccac
    21241 gcggctcgcc ggccggaccg gccagggttg gcggctcata gggatcgacc cggaagggat
    21301 cgacctccgc ctcggcggta ccgtcgagag gcttgacttc cgcgagccag tgcgcgatgc
    21361 caggagcgcg cgagaagcac tggtccaact cgcgcagcag gcgagagaac aaagctgaga
    21421 ccctatactg tagcgggaac aaagggcggg cgatcgacgt tgtgctgccg gccttgtcgt
    21481 gtgtgttcat gccagcgcag gcgaacgggc ggaccaaggg ataaccaaga ggggatcagg
    21541 aggttttggc cgtgcaagac gcagcgcata cggtaacggg caagccgagc agcttcgggc
    21601 tcgaacggca gggtctacac catgttgccg ccgtccattg gaacctgtcg gtgccgatcc
    21661 tctatgagca tgcgatccgc cggcgcgaag gcctggttgc tgccggcggc gccttcgtcg
    21721 cccgcaccgg cgccttcacg gggcgcacgc cgcgcgacaa gtacatcgtc gaggagccct
    21781 cgagcaaagc cgatgtctgg tggggcgaca tcaacaagcc ggtgagcgag gcgaccttcg
    21841 agagcatgta ccggcggctc accgcctatt tccaacggcg cgagctcttc gtccaggatc
    21901 tctatggcgg cgccgacccg aactaccggc tcaaagtgcg cgtcgtcacc gacagcgcct
    21961 ggcacagcct cttcgtccgc aacatgttca tccgcccgaa ggccgaggcg ctcggcgact
    22021 tccagccgga tttcaccatc ctgcacgcgc cgcatttctt cgccgaccac gtggccgacg
    22081 gcctcaactc cgataccttc atcttcgtca atttcgcgcg gcggctcgtg ctgatcggcg
    22141 gcagcgaata tgccggcgag atcaagaagt cggtcttcgg ctatctgaac ttcctgctgc
    22201 cggcgcgcga cgtgctgccg atgcattgct cggcgaatat cggtcccaag ggcgacagcg
    22261 ccatcttctt cggcctttcc ggcaccggca agacgacgct ctcggccgat ggcgcgcgca
    22321 tgctggtggg cgacgacgag catggctgga gccatcacgg cctcttcaat ttcgagggcg
    22381 gctgctacgc caaggtcatc aatctctcgt ccgaggccga gcccgagatc tacgccacca
    22441 tccatcgctt cggcaccgtg ctggagaatg tcatcctcga tcccgcgagc cacatgccgg
    22501 atctctccga tggcagcctc accgagaaca cgcgcgcctg ctacccgctc gacttcatcc
    22561 ccaatgccag ccacaccggc atggcgccgc accccgacaa tgtgatcatg ctgacggcgg
    22621 acgccttcgg cgtgctgccg ccgatcgcga agctcacccc cgagcaggcg atgtatcact
    22681 tcctgtcggg ctatacggcg cgcgtcgccg gcaccgagaa gggcctcggc aatgcgccgc
    22741 aagccacctt ctccacctgc tttggcgcgc cgttcatgcc gcgtcaccct tccgtttatg
    22801 ccaagatgct gggtgagcgc atctccgagc acggcgcgca atgctggctc gtcaacaccg
    22861 gctggtcggg cggcgcctac ggtgtcggca gccgcatgaa gatcgcccat acccgcgcgc
    22921 tcgtccgcgc cgcgctggcc ggcacgctca aggacgcgcc ggtgaagcag gagccgtatt
    22981 tcggcctcta tgtccccgaa agctgcggcg acgtcccgcg cgacgtgctg aacccgcgcc
    23041 acacctggtc cgacaagggc gcttacgacg agacggcgcg cgagctgacc aagcgcttcg
    23101 agagcaactt cacgaaattc gaagcctatg tcggc
//
LOCUS       DASYNS010000203         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3165022, whole genome shotgun sequence.
ACCESSION   DASYNS010000203 DASYNS010000000
VERSION     DASYNS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3165022"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..808)
                     /locus_tag="VGP50_06295"
     CDS             complement(41..808)
                     /locus_tag="VGP50_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV8027020.1"
                     /translation="MARVTAFSNRAAPETSEEREARVALAACYRLIAHFGMDDLVYTH
                     ISARVPGRPDHYFINPYGMMFHEITASSLVEVDYDGNIIWDVGYPVNKAGIVIHGAIL
                     AARPDIHCVLHTHTRAGIAVSCLKEGLLHINQASLMFHGRLGYHDYEGIALDDDERPR
                     LARDLGKHPAMILRNHGLLTVGRTVPEAFSLMYYLEQCCRVQIDLMATGGALNLLSEE
                     VCRHGAQQVADSPEPRGAREWPALLRLLDAKDPSYRD"
     gene            complement(860..2005)
                     /locus_tag="VGP50_06300"
     CDS             complement(860..2005)
                     /locus_tag="VGP50_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV8027021.1"
                     /translation="MGEAVECVVIGAGVVGLAVARALALAGHEVMVLEKERWIGAETS
                     SRNSEVIHAGLHYPKGSLKARFCVEGRDRLYAYCAARGVPHARVGKLTVACKEEEIPI
                     LEGVWRKAEENGVDDLEWVGGNEARALEPELFCRRAFFSPSTGIVDSHALMLAYQADL
                     EAAGGMVVLRAPVLSGSAGAEGFTLEVGGAEPMTLDCRMLINSGGVHAPALARRIRGI
                     PPESIPRDYFCRGVYFTLSGRSPFRRLIYPVPEPGGLGVHLTLDLAGQARFGPDVEWI
                     DRLDHTVDPRRGERFYAAIRNYWPGLRDGALQPGYAGIRPKISGPTEPAADFAVQGPA
                     DHGVAGLVNLYGIESPGLTASLALADHAVRLLDGVARSVFPEVTRTG"
     gene            complement(2026..>3039)
                     /locus_tag="VGP50_06305"
     CDS             complement(2026..>3039)
                     /locus_tag="VGP50_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
                     /protein_id="HEV8027022.1"
                     /translation="AVVSVTGGGSKSDGGQKLRGYLLDLSQSKSAAHRLALDWDPAMT
                     GFQQVTVEASDDLQNWQTWRTAAQLARLEFNGQHVDRRDIDLPDQHASYLRLTWRAPA
                     EAPVLTAVTLTAVSASYRPTPFVWSGALPPTRTSDGAYEWDFPRLIAPERLKLGLPET
                     NVLAPAELWGKGDGPPPSAWRLLAGTVVYRLDVAGREWEQDDIALSAPPVRSIKLTLD
                     ARGGGLGNGVPTVSLGLAGRQLLFLARGGGPFVLAIGNAKAELADLAPATLIPGYESS
                     AAPPISPARLGALAGMAVPPVPSSASALTPNWKTVTLWAILLTGVAGIGAMALYLLRQ
                     MKK"
BASE COUNT          490 a         1036 c         1047 g          466 t
ORIGIN      
        1 ccgtcaccgt catggtcgag gacggcccgg agcactgagc tcagtcgcga tagctcgggt
       61 ccttggcgtc gaggagccgc agcagcgccg gccattcccg cgcgccgcga ggctcgggcg
      121 agtccgcgac ctgctgcgcg ccgtgacggc agacctcctc ggacagcagg ttgagcgcgc
      181 cgcccgtcgc catgagatcg atctgaacgc ggcagcactg ctcgagataa tacatgagcg
      241 agaaggcctc gggcacggtg cgtccgaccg tgaggagacc atggttgcgc aggatcatcg
      301 ccgggtgctt gccgaggtcg cgcgccagcc ggggacgctc gtcgtcgtcg agggcgatgc
      361 cctcgtaatc gtgatagccg agacggccat ggaacatgag cgacgcctgg ttgatgtgga
      421 gcagcccctc cttgaggcag gagacggcaa tgccggcgcg cgtatgggtg tgcagcacgc
      481 aatggatgtc gggccgcgcc gccaggatcg cgccgtggat gacgatgccc gccttgttca
      541 cgggatatcc cacatcccaa atgatgttgc cgtcgtaatc gacctcgacg aggctcgacg
      601 ccgtgatctc gtggaacatc atcccgtacg ggttgatgaa gtagtggtcg ggccttcccg
      661 gcacgcgcgc cgagatatgg gtgtagacga gatcgtccat gccgaaatga gcgatgagcc
      721 ggtagcaggc agcgagcgcc acccgcgctt cgcgctcctc cgaagtctcg ggcgcggcgc
      781 ggttcgagaa agcggtgact cgggccatga tctcctcgtc ggttgtctcg tcccctgagg
      841 gggcgcgttt gctgccagcc tagcctgtcc gcgtgacttc cgggaatacc gatcgcgcaa
      901 ccccatccag cagcctcacc gcgtgatcgg cgagcgccag cgaggccgtg agccccggcg
      961 attcgatgcc gtagagattg accagaccgg ccacgccatg atcggcgggg ccttgcacgg
     1021 cgaagtcggc cgcgggttcc gtcgggccgc tgatcttggg gcggatgcca gcatagcccg
     1081 gctgcagcgc gccgtcccga aggcccggcc agtaattgcg gatcgctgca tagaagcgct
     1141 cgccgcggcg cggatcgacc gtgtggtcga ggcggtcgat ccattcgaca tcgggaccga
     1201 agcgcgcctg tcccgcgaga tcgagcgtca gatggacgcc gagcccgccc ggctccggca
     1261 cgggatagat gaggcggcga aagggcgagc gccccgacag cgtgaaatag acgccgcggc
     1321 agaaataatc acgcgggatc gattcgggag gaatgccgcg gatccggcgc gccagcgccg
     1381 gcgcatggac gccgccgctg ttgatcagca tccggcagtc gagcgtcatc ggctccgcgc
     1441 cgccgacctc gagcgtgaag ccctcggcgc ccgcgctgcc cgaaagcacc ggcgcgcgca
     1501 gcaccaccat gccgccggcg gcttcgagat cggcctggta ggcgagcatc aaggcgtggc
     1561 tgtcgacgat cccggtcgac ggcgagaaga aggcgcggcg gcagaagagc tcgggctcga
     1621 gcgcgcgggc ctcgttgccg ccgacccatt cgaggtcgtc gacgccgttc tcctcggcct
     1681 tgcgccacac gccttcgagg atcggtatct cttcttcttt gcaggcgacg gtgagctttc
     1741 cgactcgcgc gtgcggcacg ccgcgcgcgg cgcaatacgc atagagccgg tcgcgtccct
     1801 cgacgcagaa ccgcgccttg aggctgcctt ttggataatg cagccccgca tggatcactt
     1861 cgctgttgcg tgacgacgtc tcggcgccga tccagcgctc cttctcgagc accatgacct
     1921 cgtgcccggc gagcgccagc gcccgcgcca cggcgaggcc gaccacgccc gcgccgatca
     1981 cgacgcattc gaccgcttcg cccatcgcct ggccttatgc cgcgcctatt tcttcatctg
     2041 ccgcagcaga tataacgcca tggcgccgat tccggcgacc cccgtcagca ggatcgccca
     2101 taaggtcacg gtcttccaat ttggcgtaag cgcgctggcg ctcgacggta cgggcgggac
     2161 cgccatcccg gcgagggcgc cgagccgcgc cggcgagatc ggcggcgcgg ccgagctctc
     2221 atagcccgga atcaaggtgg cgggcgccag atccgccagc tcggccttgg cattgccgat
     2281 agcgaggacg aaggggccgc cgccccgcgc gaggaacagc agttgccgtc ccgcaaggcc
     2341 gagcgacacg gtcgggacgc cgttgcccag accgccgccg cgggcgtcga gggtaagctt
     2401 gatcgaccgg accggcggcg ccgacagcgc gatgtcgtcc tgctcccatt cccggcccgc
     2461 gacgtcgagg cgatagacga cggtccccgc gagcaaccgc caggccgatg ggggcggtcc
     2521 atcgcccttg ccccagagct cggccggcgc cagcacattg gtttccggca atcccagctt
     2581 cagccgttcg ggcgcgatga gccgggggaa gtcccattca tacgcgccgt cgctcgtccg
     2641 cgtcggcggg agcgcgcccg accagacgaa gggagtcggg cgatagctcg cggagacggc
     2701 cgtcaaagtg acggcggtca ggacgggggc ttcggccggc gcgcgccagg tgagccgtag
     2761 atagctcgca tgctgatccg ggagatcgat gtcgcggcgg tcgacatgct gaccgttgaa
     2821 ttcgaggcgc gccagctgcg cggcggtccg ccaggtctgc caattctgca ggtcgtcgct
     2881 cgcctccacc gtcacctgct ggaacccggt catcgccggg tcccagtcga gggcgaggcg
     2941 gtgcgcggcg gatttgctct ggctcagatc gagcagatat ccccgaagct tctggccgcc
     3001 atcgctcttg cttccgccgc ccgttaccga cacgacggc
//
LOCUS       DASYNS010000204         4213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4256422, whole genome shotgun sequence.
ACCESSION   DASYNS010000204 DASYNS010000000
VERSION     DASYNS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4256422"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VGP50_06310"
     CDS             complement(<1..672)
                     /locus_tag="VGP50_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027023.1"
                     /translation="MPARDIARRKIRSFALIVAIFVVLLLIALAASWYAIEVVNSTRA
                     YATGEAHYSKAQKIAVLSLHRYADSRLEIDYAAFLAATSIERGDLQARLALESQPVDI
                     VAAEAGFLEGGNHAEDLDSMIWLFRGFSWWGPFAEAVADWRESDPLLRELIALGARLH
                     ERVVEGTLDRQTRDWLLGDVDRVDYRLSALETAFATHIGEAARGATRLVITALSIATI
                     VLWTIG"
     gene            1331..4000
                     /locus_tag="VGP50_06315"
     CDS             1331..4000
                     /locus_tag="VGP50_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV8027024.1"
                     /translation="MRLFRTPVIALALALVVAFTLGKPAAAEEKDVLRATLPNGLRVI
                     LVRNTLAPVVSTVMNYLVGADEAPRGFPGMAHAQEHMMFRGSPGLSTDQLADIGSLMG
                     GRFNAATRQTATQYFFTVPASALDVALHVEAVRMRGVLDSESGWAQERGAIEQEVAQD
                     LSDPRYVLLTKLREVMFAGTPYAHDPLGTRPSFDRTTGAMLHAFHDRWYAPNNAILVV
                     AGDLDLAATLAEVTRLFGAIPRKTLPERPAVALRPVSAQTLKLDTDLPYALDVIALRL
                     PGLESPDYPAAELLADVLSSHRGELYGLVPRGAALYADFLADAQPKAGLGYAVMAFPA
                     GGDGAALAEEARRILAGIAKDGVSPDLVAAAKLEERRTAELQKNDIEGLATVWSEAVA
                     INGLTSPDEDLERIEKVTVADVDRVARQYLDLDHAVMAVLTPQGSGKAVASSGFGGQE
                     SIALGDAKPAVLPKWAETALGRLAVPASTVHPVVSKLANGLTLIVQPEDVSDTVSVYG
                     HIENRPELEVPAGKEGLSEVLDQLFSYGTERLDRIAFQRALDDIGADETAGPDFSLKV
                     LAENFDGGVELLAENELYPRLPADAFAVVKRQVKETVAGRLESADYMAQQELRAELFP
                     KGDPSLREARPETVDRLTLEDVRDYHQNAFRPDLTTIMVIGKVTPEEAKAVISHHFGA
                     WKSAGEKPQTLLPPVPANPPGTTEVPDKSRVQDDVVIAETVGLVRSDPDYYALELGNA
                     VLGGTFYATRLSRDLRLKTGLVYSVNSFFDVKRTRGLYFLQYACDPQNVSRVRNIVVR
                     ELRAMQQTPVSALELRRAKAFLLRQMPLEEASVEEIAEQTLQRASLELPLDEPAIAAQ
                     HYLALSPAEVQAAFAKWLRPDGLAQVSEGPHPR"
BASE COUNT          696 a         1421 c         1421 g          675 t
ORIGIN      
        1 gccgatcgtc cagaggacga tggtggcgat gctcagcgct gtgatcacga gacgggtcgc
       61 gccgcgcgcg gcctcgccga tatgggtggc gaacgcggtt tcgagcgcgg aaaggcggta
      121 gtcgacgcgg tcgacatcgc cgagcagcca gtcgcgggtc tggcggtcga gggtcccttc
      181 gacgacccgc tcgtgcaggc gtgcaccgag cgcgatcagc tcccgaagca aggggtcgct
      241 ttcgcgccag tcggcgaccg cctcggcgaa gggaccccac catgagaacc ctcggaacag
      301 ccagatcatc gagtcgaggt cttctgcatg gttgccgccc tcgaggaagc cggcctcggc
      361 ggcgacaatg tcgacgggct ggctctcgag cgcgaggcgg gcttgcaggt cgccgcgctc
      421 gatcgacgtc gcggcgagaa aggccgcgta gtcgatttcg agccgcgaat cggcatagcg
      481 atgaagactg agcacggcga tcttctgcgc ctttgaatag tgagcttcgc cggtcgcata
      541 ggcgcgcgtg ctgttgacca cctcgatcgc gtaccagctg gcggccagcg cgatcaacaa
      601 gagcacgacg aagatggcga cgatgagcgc gaagctgcgg atcttgcgcc gcgcgatatc
      661 ccgggcaggc aagtccccag gcgatcctcc gggcgtcggc ggcactgcgg cgagcatcac
      721 acgccctcct caacgaccgg cccgctgctg cgcgagcgat ccatggttat cgttctgttg
      781 cgtcgtggct cggcagcggg cgccgcggac gattatccca cgcgtgtgct taatcaattc
      841 taaaccggct cgtcccgcgc cgagggactg cctcgacgcg ccgtgaaggg cctccgtccc
      901 gaatgctgct gcagggagat atggagacgc gtcgccaagc gacaatgagc cgcaggcgcg
      961 agcttggccc ccatttgccg acgtcgctcg cgaatgccga ccgccatccc cgagaaagac
     1021 gaacgccgag accgcggagg atacgcggag gacgcggagg aacagccatc cctttatgcc
     1081 tcctgcgtat cctccgcgac ctgtgcgctc gctaatgtgc tctcgcactg cgccgcgccg
     1141 atcgcgctcg cgtgaccggc caggggattg gcaagcgctc gcgcgatcta tactttcgca
     1201 gcggcagttt atctccgccg tcttcagcgt ccgtcgctcg ccgccccctc atccttccct
     1261 ctcccccgat gggggcgagg ggctaaatat gttggcgacg gggacagatc gttttcaggg
     1321 gaatcagtat atgcgactct tccggacgcc agtgatcgcc ctcgccctcg ccctcgtggt
     1381 cgcgtttacc ctcggcaagc ccgccgcggc ggaagagaag gatgtgttgc gggcgacgct
     1441 gccgaacggg ctccgcgtca tcctcgtgcg caacacgctg gcgccggtgg tcagtacggt
     1501 gatgaactat ctcgtcggcg ccgatgaggc gccgcggggc tttcccggca tggcgcatgc
     1561 gcaggagcat atgatgttcc gcggcagccc cgggctctcc accgaccagc tcgccgacat
     1621 cggcagcctc atgggcggac gcttcaatgc cgcgacgcgc cagacggcga cgcagtattt
     1681 cttcacggtg ccggcgtcag cgctcgacgt ggcgctccat gtcgaggccg tccggatgcg
     1741 gggcgtgctc gacagcgaga gcggctgggc gcaagagcgc ggcgccatcg agcaggaagt
     1801 ggcgcaggat ctttcggatc cgcgctacgt ccttctgacc aagctgcgcg aggtgatgtt
     1861 cgcgggcacg ccctatgcgc acgacccttt gggcacgcgc ccgtccttcg accgcacgac
     1921 cggagcgatg ctgcacgcgt tccacgatcg ctggtacgcg cccaacaacg ccatcctcgt
     1981 ggtcgccggc gatctcgatc tcgcggcgac gctcgccgaa gtgacgcggc tcttcggcgc
     2041 gatcccgcgc aagacgctgc cggagcggcc ggcggtcgcg ctgcgtccag tcagcgcgca
     2101 aacgctcaag ctcgatacgg acctccctta cgcgctcgac gtgatcgcgc tgcgcctgcc
     2161 ggggctcgaa agcccggatt atccggcggc cgagctgctc gccgacgtgc tcagcagcca
     2221 tcgcggcgag ctctacggcc tggtgccgcg cggcgcggcg ctctacgcgg atttcctggc
     2281 ggacgcacaa ccgaaggcgg ggctcggcta cgccgtcatg gcctttcccg ccggcggcga
     2341 cggcgcggcg ctcgccgaag aggcgcgccg catcctcgcc gggatcgcca aggacggcgt
     2401 ctcgcccgac ctggtggcgg cggcgaagct cgaggagcgg cgcacggccg agcttcagaa
     2461 gaacgacatc gagggcctcg cgacggtgtg gtccgaagcg gtcgccatca acgggctcac
     2521 ctcgccggac gaggatctcg agcgcatcga gaaggtgacc gtcgccgacg tcgatcgggt
     2581 ggcgcgccaa tatctcgatc tcgatcatgc ggtgatggcg gtgctcacgc cgcaagggtc
     2641 gggcaaggcc gtcgcctcga gcggcttcgg cggccaggag agcatcgcgc tcggcgatgc
     2701 caagccggcg gtgctgccga aatgggcgga gacggcgctg ggccgcctcg ccgtgccggc
     2761 ctcgacggtg catcccgtgg tgagcaagct cgccaacggc ctcaccctca tcgtgcagcc
     2821 cgaggatgtc agcgacacgg tgagcgtcta cggccatatc gagaaccggc ccgagctcga
     2881 ggtcccggcg gggaaggaag ggctctccga agtgctggac cagctcttct cctacgggac
     2941 cgagcggctc gaccgcatcg ccttccagcg cgcgctcgac gacatcggcg ccgacgagac
     3001 ggcgggcccg gatttctctc tcaaagtgct cgcggagaat ttcgatggcg gcgtcgagct
     3061 tctcgcggag aacgagcttt atccgcgttt gcccgccgac gccttcgccg tcgtcaagcg
     3121 gcaggtcaag gagacagtgg ccgggcgcct cgaaagcgcg gactacatgg cgcagcagga
     3181 gctgcgcgcg gagctctttc cgaaaggcga tccaagcctg cgcgaggcgc ggccggaaac
     3241 cgtcgaccgg ctgacgctcg aggatgtgcg cgattatcac cagaacgcct tccgccccga
     3301 tctcaccacg atcatggtga tcggcaaggt cacgcccgaa gaggcgaagg cggtgatctc
     3361 ccaccatttc ggcgcctgga agagcgccgg cgagaagccg cagacgctgc tgccgccggt
     3421 gccggccaac ccgccgggga cgaccgaagt cccggacaag agccgcgtgc aggacgacgt
     3481 cgtgatcgcc gagaccgtcg gtctcgtgcg ctccgatccc gactattacg cgctcgagct
     3541 cggcaatgcc gtgctcggcg gcacgttcta tgcgacgcgg ctgagccgcg acctgcgctt
     3601 gaagaccggg ctcgtctatt cggtcaactc cttcttcgac gtgaaacgca cgcgcggcct
     3661 ttatttcctg caatacgcct gcgatccgca gaatgtctcg cgggtccgca acatcgtcgt
     3721 ccgcgagctg cgggcgatgc agcagacgcc ggtctccgcg ctcgagctgc gccgggcgaa
     3781 agcgttcctg ctgcgtcaga tgccgctcga ggaggcgagc gtcgaggaga tcgcggagca
     3841 gacgctgcag cgcgcgagcc tcgagctgcc cctcgacgag cccgccatcg ccgcgcagca
     3901 ttatctcgcc ttgagccccg cggaggtgca ggcggccttc gcgaaatggc tccgtcccga
     3961 cggcctcgcg caagtgagcg aaggaccgca ccctcgctaa agcacgtctc gttcatctgg
     4021 aatcgggttt aacaacctcg tcgtccccgc gacgaggaat ttggtttagt tgaaagcgac
     4081 atggccctag gccgcgttac atacatctcg tgacttggtc gaggagcgaa ccctttgtca
     4141 gcgatcgcgg aacgcagagg gcgcggtggt tacgcggagg tcgccgagga tggctgtcct
     4201 ccgcgccctc cgc
//
LOCUS       DASYNS010000205         7226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2386160, whole genome shotgun sequence.
ACCESSION   DASYNS010000205 DASYNS010000000
VERSION     DASYNS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2386160"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VGP50_06320"
     CDS             <1..261
                     /locus_tag="VGP50_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV8027025.1"
                     /translation="LRPILMTAFAFILGVIPLMIASGAGAASRQSIGTTVFGGMLAAT
                     LLSLALVPVFYAVIERLREGRHASLGTQRVPHGRDAVPAAGD"
     gene            548..1693
                     /locus_tag="VGP50_06325"
     CDS             548..1693
                     /locus_tag="VGP50_06325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV8027026.1"
                     /translation="MRVSRITLGLAATVTSAALLAFILFGNADHRASAAGQDKPPPGL
                     AVPVFAVVKKAVPVDLDYVAQAEAIRSVTLQAKVTGYLLTQGAADGANVTAGDLLYRI
                     DPRDYQAALDQAKAQAQRDVAQREYADANSHRNQLLSKNGDVSVDTYQQSVSALHQAE
                     ASIAADRAAIETAALNLGYTEIRAPFAGRLSRSQVHEGALISAAGTQLNTLVQLDPIY
                     VSFNPPETDLAAIARAAAQGPVTAEVTIGDDATLRFAGTLSFLDNQVDHATGSITARA
                     TIANPQQNLLPGQYARVRLHVGEQPDALLVPEVAIGSSQLGRYVYLVGKDDKAEQRIV
                     TLGRTYGDLIVVEQGLAAGETVIVGDLQKLRPGVPVAPQPAPGAGGA"
     gene            1690..3156
                     /locus_tag="VGP50_06330"
     CDS             1690..3156
                     /locus_tag="VGP50_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEV8027027.1"
                     /translation="MMRRAHSALTLAASAALLVGCEVGPDFQRPAAPQATGYTPEPLP
                     SSTAAADTHGGETQLLIAGRDIPGDWWALYRSPALNALIEDAIKANPNLDAARAALRQ
                     AHELELAGVGALFPTVSAGASATREKESLAGFGLGASSSIFSVNSASLTVDYALDIFG
                     GTRRNIESLAAETEFERFELEATYLSLTANVVTAAVAEASLRGQIAATQQIIDIESQE
                     LERLNRDFAAGAANNTAVLQQAATLAQTRATLPPLEKALAQTRDLLAVLLGRLPSDMP
                     ATEFALADLELPQELPLTLPSLLVEQRPDIRAAEAQLHAASAQIGVALANMLPQLSLS
                     GSLGTISTGAIFQPGTGVWSLGGSLVQPLFEGGTLLHRKRATEAAFDQAAAQYRQTVL
                     IAFQNVADSLRALELDADALNAQLAAERAAADSLTVARRQFELGSGTYLTLLNAEQTY
                     QQAHITLVEAQAARFSDTAALFQALGGGWWNRTDFASR"
     gene            3200..4594
                     /locus_tag="VGP50_06335"
     CDS             3200..4594
                     /locus_tag="VGP50_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3734 domain-containing protein"
                     /protein_id="HEV8027028.1"
                     /translation="MPDTHDTHRASAPARNSRRKSHQAQQRPPFERIALLLQGGGALG
                     AYQAGVYQALAEAHLHPDWVAGISIGAINSALIAGNPPGLRIEKLREFWELVTSASLH
                     GGAEPFAAPARSHPLRDLLREAMAAAASLGATSFALISDQQLAAQVDGLRGVWEGLAA
                     RAFSGWSPDGGCALPRGDVARSLANQMSAAFALAFGAPGFFAPRLPLPWLQPAGTLEA
                     TSYYDTTPLKATLERLVDFDRINSGAMRFSIGAVNVLTGNFVYFDNATHVIGPEHVMA
                     SGALPPGFAPVEIEGEYYWDGGLVSNTPLHWVLESEPRQDTLAFQVDLWSARGVFPRS
                     MPEVMTRQKEIQYSSRTRAASDRFKHGQKLRHAFSTLLRKLPQELRDTPEFALLSPSA
                     SHKVYNLVQLIYRSKRYEGNSKDYEFSRPSMEEHWRAGHQDTIRTLRHPEVLERPTST
                     EGVLTFDLAYDGRE"
     gene            4649..5110
                     /locus_tag="VGP50_06340"
     CDS             4649..5110
                     /locus_tag="VGP50_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027029.1"
                     /translation="MSIWTLSPNSHSPTLASLKPLAAALARWALAAMLAACVLGGGYA
                     LQQVIETPGQVIRHDLARAAARLAQLPSVMPRDRVEQTISGYFSGYRASIDATGFPAS
                     VSVTLRDLDPNTCRDAYRRASRIEGTVVVAIDEPGARMCGDRTALTWRMMP"
     gene            complement(5216..5431)
                     /locus_tag="VGP50_06345"
     CDS             complement(5216..5431)
                     /locus_tag="VGP50_06345"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEV8027030.1"
                     /translation="MRTRTSRYSASRLLRVVVAIGHPHDATIDALARWIAALPQKGLV
                     LVPVSAIVRERSEEAMGTMSPAPASGR"
     gene            5469..5963
                     /locus_tag="VGP50_06350"
     CDS             5469..5963
                     /locus_tag="VGP50_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027031.1"
                     /translation="MDVGQHWIPLGDRPPEPVPVLLDPEEPKARKIWIGSWSRKPAAG
                     IRLNAAGGSVDRRYQVIRRLEPFLADEMGDKRLELPGRRARLTHTGGSKSVGGWADLS
                     LRLAGGANGGNYLGAHARQESARVFDPIMAQIFSTLLELPRQVRGHVRHRRYNRGDHA
                     VGNI"
     gene            complement(6276..>7226)
                     /locus_tag="VGP50_06355"
     CDS             complement(6276..>7226)
                     /locus_tag="VGP50_06355"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEV8027032.1"
                     /translation="WLALPPRPAAPVVVAVAPKAAVEPTAPAKAAAPGPVRPAAMVLP
                     PVPPAPRAEPAWERFAVAAPPAAGRPRIALVIDDLGVDKKRSWRTIALKGPLTLSFLA
                     YASDLPQMAQAARRAGHELMVHVPMEPMSAAENMGPNGLAIGLGRDEVLRRLRWDLGR
                     FDGYVGINNHMGSRFTSDAASMTLVMEELKTRGLLFVDSRTTAATAGADVARRLGVPC
                     AARDVFLDNEADAPAIGERLRHLERLAREHGTAIAIGHPHDATIDALAHWIATLPQKG
                     LVLVPVSAIVRERSEEAMGTMSRSPASAGERGTGREATGG"
BASE COUNT         1145 a         2616 c         2336 g         1129 t
ORIGIN      
        1 ctccgtccca tcctcatgac ggccttcgcc ttcattctgg gcgtgatccc gctgatgatc
       61 gcctcgggcg cgggcgcggc gagccgccaa tcgatcggca ccaccgtctt cggcggcatg
      121 ctcgccgcca cgcttctgag cctggccctc gtgccggtct tctatgcggt catcgaacgc
      181 ctgcgcgagg gccgtcatgc gtccctcgga acacaacgcg taccccacgg ccgcgacgcc
      241 gtgccggccg ccggcgatta aaccatgtgg acattcacgt ccgaccaatc cccaaacatc
      301 ccctctcccg catcgcggga gagggaggga cccagcgcgc agcgctggga gggtgagggg
      361 acctgtcagg caaagcgcgt ttacttgaac cgtcccctca cccggcgcgc gcttcgcgcg
      421 cgccaccctc tcccgcgatg cgggagaggg gcaaggacac gcatccatgc gaatgtccac
      481 aggacgtagc ccatgtcccg ttccagcaca ctcaagctgg acatgctctg aaccagaaag
      541 agaaccgatg cgcgtctctc gaatcactct cggattggca gcgacggtca cttcggccgc
      601 tctgctcgcc ttcatcctct tcggcaacgc agaccatcgc gcgagcgccg ccggccagga
      661 caagcccccg cccggccttg ccgtgccggt ctttgccgtc gtcaagaaag ccgtgcccgt
      721 cgatctcgac tatgtggcgc aggcggaggc gatccgcagc gtcaccttgc aggcgaaggt
      781 caccggctat ctcctcaccc aaggcgccgc cgacggcgcc aatgtgacgg cgggcgatct
      841 cctctatcgc atcgatccgc gcgactacca ggccgccctc gaccaggcca aggcgcaggc
      901 gcagcgcgat gtggcgcagc gcgaatatgc cgacgccaat tcgcaccgca accagttgtt
      961 gagcaagaat ggcgacgtgt cggtcgacac ctatcagcaa tcggtgagcg cgctgcacca
     1021 ggccgaggcc tcgatcgcgg ccgaccgcgc cgcgatcgag acggcggcgc tcaacctcgg
     1081 ctacaccgag attcgcgcgc ccttcgccgg ccgcctcagc cggagccagg tgcatgaggg
     1141 ggcgctcatc agcgccgccg gcacgcagct caacaccctg gttcagctcg atccgatcta
     1201 cgtctccttc aatccgcccg agaccgacct cgcggccatc gccagggccg cggcgcaagg
     1261 acccgtaacc gccgaggtga cgatcggcga cgatgccacg ctgcgtttcg ccggcacgct
     1321 gagcttcctc gacaatcagg tagaccacgc caccggaagc atcaccgcgc gcgccaccat
     1381 cgccaacccg cagcagaacc tgttgcccgg ccaatatgcg cgggtgcggc tccatgtcgg
     1441 cgagcagccg gacgcgctcc tcgttccgga ggtcgccatc ggcagcagcc agctcggccg
     1501 ctacgtctat ctcgtcggca aggacgacaa ggcggagcag cgcatcgtca cgctcggccg
     1561 cacttacggc gatctcatcg tggtggagca gggcctggcc gcgggcgaga cggtgatcgt
     1621 cggcgacctg cagaagctgc ggcccggcgt gccggtcgcg ccgcaaccgg cgcccggtgc
     1681 cggaggcgca tgatgcgccg cgctcattcc gccctcaccc tcgcggcgag cgcagcgctg
     1741 ctcgtcggct gcgaggtcgg ccccgatttc cagcgcccgg cggcgccgca ggccacgggc
     1801 tacacgccgg agccgctgcc gtcgtcgacg gccgcggccg atacgcatgg cggagagacg
     1861 cagctcttga tcgcgggacg cgacattccc ggcgattggt gggcgcttta ccgctcgccc
     1921 gccctcaacg cgctgatcga ggacgcgatc aaggccaatc ccaatctcga cgcggcgcgc
     1981 gccgccttgc ggcaggcgca tgagctcgag ctcgccggcg tcggcgcgct ctttcccacc
     2041 gtttcggccg gcgccagcgc gacgcgggag aaagagtcgc ttgccggctt cggcctcggc
     2101 gccagcagca gcatcttcag cgtcaattcc gcctcgctga ccgtcgatta cgcgctcgac
     2161 atcttcggcg gcacgcgccg caatatcgaa tccctcgcgg ccgagaccga gttcgagcgc
     2221 ttcgagctcg aagcgaccta tttgagcctg acggccaatg tcgtgacggc ggccgtcgcg
     2281 gaagcgtcgc tgcggggcca gatcgccgcg acgcagcaga tcatcgacat cgagagccag
     2341 gagctcgagc gtctgaaccg cgatttcgcg gccggcgccg ccaacaacac ggcggtgctg
     2401 cagcaggcgg cgaccttggc gcagacgcgc gccacgctgc cgccgctcga gaaggcgctg
     2461 gcccagacgc gcgatctgct ggccgtcctg ctcgggcggc tccccagcga catgccggcg
     2521 acggaattcg cgcttgccga tctcgagctg ccccaggagc tgccgctgac gctgccctcg
     2581 cttctcgtcg agcagcggcc cgacatccgc gccgccgagg cgcagcttca tgcggcgagc
     2641 gcgcagatcg gcgtcgccct tgccaacatg ctgccgcagc tcagcctctc gggatcgctc
     2701 ggcacgattt ccacgggcgc gatcttccag ccgggcaccg gcgtctggag tctgggcggc
     2761 tcgctcgttc agccgctctt cgagggcggc acgctgctgc acaggaagcg cgccaccgag
     2821 gcggcgttcg accaggcggc cgcgcaatat cgccaaaccg tgctcatcgc cttccagaat
     2881 gtcgccgact cgctgcgcgc gctcgagctg gacgccgacg cgctcaacgc gcagctcgcc
     2941 gcagagcgtg cggccgccga cagcctcacc gtcgcgcggc gccagttcga gttgggctcc
     3001 ggcacctatc tgacgctgct caacgccgag cagacctatc agcaggcgca tatcacgctg
     3061 gtcgaggcgc aggcggcgcg cttctccgat accgctgcat tgtttcaagc gctgggcggc
     3121 ggctggtgga accggacgga tttcgcgtcg cgataggcgc ccctggttca gcgacgacgt
     3181 gaaaagaatg gagcgaccca tgcccgacac ccatgacacg catcgcgcaa gcgcgccggc
     3241 gcggaattcg cggcgaaaga gccaccaggc gcagcagcgc ccgcccttcg agcgcatcgc
     3301 tctgctgctg cagggcggtg gcgcgctcgg tgcctatcag gcgggcgtct atcaggcgct
     3361 ggccgaggcg catctccatc ccgattgggt cgccggcatc tcgatcggcg ccatcaattc
     3421 cgcgctcatc gccggcaatc cgccggggct acggatcgag aagctgcgcg aattctggga
     3481 gctcgtcacc tcggcgtcgc tccacggcgg cgccgagccg ttcgctgcgc ccgcccggag
     3541 ccatcccctg cgcgatctcc tgcgcgaggc gatggcggcg gcggcctcgc tcggcgccac
     3601 gagcttcgct ttgatctcgg accagcagct ggcggcgcag gtcgacgggc ttcgcggcgt
     3661 ctgggaaggc ctcgcggcgc gcgctttttc gggctggtcg cccgatggcg gctgcgccct
     3721 gccgcgcggc gacgtcgcgc gcagcctggc gaaccagatg agcgccgctt tcgctctggc
     3781 cttcggcgct cccggcttct tcgcgccgcg cctgccgctc ccctggctgc agcccgccgg
     3841 tacgctcgag gcgacgagct actacgacac cacgccgctc aaggccacgc tcgagcgtct
     3901 cgtcgatttc gaccgcatca acagcggcgc gatgcgcttc agcatcggtg cggtcaacgt
     3961 cctcaccggc aacttcgtct atttcgacaa cgcgacccat gtcatcggcc ccgagcatgt
     4021 gatggcgagc ggagcgctcc cgcccggctt cgcgcccgtc gagatcgagg gcgagtatta
     4081 ctgggatggc ggcctcgtct cgaacacgcc cttgcattgg gtgctcgaga gcgagccgcg
     4141 ccaggacacg ctcgcctttc aggtcgatct ctggagcgcg cgcggcgtct tcccgcgcag
     4201 catgccggag gtgatgaccc gccagaagga gatccagtat tcgagccgca cgagggccgc
     4261 ctccgaccgc ttcaagcacg ggcagaagct ccggcacgcc ttctcgaccc tgctccgtaa
     4321 gctgccccag gagttgcgcg acaccccgga attcgctctg ctcagtccgt ccgccagtca
     4381 caaggtctac aacctcgttc agctcatcta tcgctcgaag cgctacgagg gcaattcgaa
     4441 ggattacgaa ttctcgcggc cgagcatgga ggagcactgg cgcgccggcc accaggacac
     4501 catccgcacg ctccggcatc ccgaagtcct ggaacgcccg accagcaccg aaggcgtcct
     4561 caccttcgac ctcgcttatg acggtcgcga atgacagcgt ccccgcgaaa gcggggacgc
     4621 gagagccgca ccgaccagga gacgcaccat gtcgatttgg acactctcgc caaattccca
     4681 ttctcccacg ctcgcctccc tcaagccgct ggccgcggcg ctcgcccgat gggcgctcgc
     4741 ggcgatgctg gccgcttgcg tcctcggcgg cggctacgcg ctgcagcagg tgatcgagac
     4801 gcccggccaa gtcattcgcc atgacctcgc gcgcgccgcc gcaaggctcg cgcagctgcc
     4861 gagcgtcatg ccgcgcgatc gcgtggagca aacgatatcc ggctatttct ccggatatcg
     4921 cgcgagcatc gacgcgacgg gatttccggc ttcggtatcc gtgaccctcc gggatctcga
     4981 ccccaacacc tgccgcgacg cctatcgcag agcctcacgc atcgagggca cagtggtggt
     5041 cgccatcgac gagcctggcg ctcgcatgtg cggggaccgg accgcgctga cctggcgaat
     5101 gatgccctaa ggcgagcgcg cgccagcgcg ctcgtccttg gcggcgacag cgattgcgca
     5161 gcaatcgcga aagccgcacc gagcagaacc ggctcccctt cgccgcgcac caaacctatc
     5221 gcccgctcgc cggcgcgggg ctcatcgtcc ccatcgcttc ttccgaccgc tcccgcacga
     5281 tggcgctcac cggcaccagc acgaggccct tctgcggcaa cgcggcgatc cagcgcgcga
     5341 gcgcgtcgat cgtcgcgtca tggggatggc cgatggctac gacaactcga agtaagcgtg
     5401 aggcgctata gcgggacgtc ctcgtcctca tcggctgcga ggcggccgtc gatagacgcc
     5461 gtctcgaaat ggacgttggt cagcactgga taccccttgg cgatcgacca cccgaaccag
     5521 taccggtact ccttgatcca gaggaaccca aagcgcgcaa gatctggata gggtcgtgga
     5581 gccggaagcc cgcggcgggg atccgcctca atgcggcggg cggcagcgtc gaccgccgct
     5641 accaggttat tcgccgcctc gaaccgttcc ttgctgatga aatgggcgat aagcgcctcg
     5701 agctgcctgg acgccgtgcg cgtctgacgc atacgggcgg ctcaaagtcg gttggcggct
     5761 gggcggattt atctcttcgg ctggcgggcg gcgccaatgg cggcaactat cttggcgcgc
     5821 atgcgcgcca ggaaagcgcc cgtgtcttcg atccgatcat ggcgcagatc ttcagcactc
     5881 tcctggagct gccgcgccag gtccgggggc acgttcgcca ccggcgctac aaccggggcg
     5941 atcacgcggt tggaaacata tgacgacacc tgtgggggtg ctttctgggc cacgcttccg
     6001 ttcctgtgcc ggtgatatag gtgctcgcaa ggcgtgatgc gagcctagcc caccctgtcg
     6061 ggatgcgaac cagattttca actataccga cattgactct agaaaaggtc aactaaacaa
     6121 gcgcccgagg atgctccttg taggaccgat cggccaaacc cattcgattt atatcgaatt
     6181 tgctgcgttg ttcctgtgga gcggcgtcgc tctcgggctc ggccctgcgt ggcttccgac
     6241 tcgctagact ggctctagga gtctccgagc aaccctcacc cccccgtcgc ttcgcgcccg
     6301 gtccctcgct ctcccgcgct tgcgggcgag cggctcatcg tccccatcgc ttcttccgac
     6361 cgctcccgca cgatcgcgct caccggcacc agcacgaggc ccttctgcgg cagcgtcgcg
     6421 atccagtgcg cgagcgcgtc gatcgtcgcg tcatggggat ggccgatggc gatcgcggtg
     6481 ccgtgctcgc gcgcgagccg ctcgagatgg cgaagacgct cgccgatcgc cggcgcgtcg
     6541 gcctcgttgt cgaggaagac gtcgcgcgcg gcgcagggga cgccgagccg gcgtgcgaca
     6601 tcggcgcccg cggtcgccgc ggtggtgcgg gaatcgacga agagcaggcc gcgcgtcttc
     6661 agctcctcca tgacgagcgt catgctcgcg gcatcgctgg tgaagcggct gcccatatgg
     6721 ttgttgatgc cgacatagcc gtcgaagcgg ccgagatccc agcgcagccg ccgcagcacc
     6781 tcgtcgcggc cgagaccgat cgcaagcccg ttcgggccca tattctcggc cgcgctcatc
     6841 ggctccatcg gcacatgcac catgagctcg tgcccggcgc ggcgcgccgc ttgcgccatt
     6901 tgcggcaagt cgctggcata ggcgagaaag gacagcgtga gcggcccttt gagagcgatc
     6961 gtccgccagc tccgcttctt gtcgacgccg aggtcgtcga tgacgagcgc gatgcgcggg
     7021 cgtccggcgg cgggcggcgc tgccacggca aagcgctccc aggccggctc cgcccggggc
     7081 gccggcggca ccggcggaag gaccatcgcc gcggggcgga ccgggcccgg ggcggcggcc
     7141 ttggccgggg ccgtgggctc gaccgccgcc ttgggcgcta cggcgaccac gacgggcgcc
     7201 gccggccgcg gcggcaaggc cagcca
//
LOCUS       DASYNS010000206         9940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5348357, whole genome shotgun sequence.
ACCESSION   DASYNS010000206 DASYNS010000000
VERSION     DASYNS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9940
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5348357"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VGP50_06360"
     CDS             complement(<1..1098)
                     /locus_tag="VGP50_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEV8027033.1"
                     /translation="MTTDRKSPLALLRTALVGLTMAAAVASMGRADAQTVTTPGGTFP
                     AETFQGVAGNGITTFLGIRYAAAPTGNLRFAAPTPPAAVTGTVEATQFGSPCPQSASP
                     FGTASNDEDCLFLNVYVPGSSVSARNSLPVMVFYHGGAFVFGQGSLYNPTQMAMEGDT
                     IVVTINYRLGILGYLADAALDAVSPNNASGNYGLRDQQFALKWVQQNISAFGGNPRNV
                     TIFGESAGGFSVCANIVSPAAKGLFQAAITESGPCATPLPTKAAAEAGGAQIVSALDC
                     ADATAAATVACLRGLSIAQILAVQNAVTGANSLASLFAFFPDVDGVVIPQEPITAIAL
                     GEYNHVPVIEGTNHDEGRLFVALAFDLNPAVG"
     gene            1399..2175
                     /locus_tag="VGP50_06365"
     CDS             1399..2175
                     /locus_tag="VGP50_06365"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEV8027034.1"
                     /translation="MSDTAERFERINAAPAYQLVAEAIEKGITSGRLRAGEPLGTEAE
                     LVRQFGVNRSTVREGIRMLEQAGLVHRDSSRRLTVGVPHYDRLASRRTRALVMHEVTF
                     RELSEAVLVLDLAAVEQAALRATPAVIAELDANIAKTEAAAGNWPVVTELDTQWHELF
                     YKAAGNRVLQLAREPAPLLALSVTQMILEQVKEALPRLLVAHRTITEAVRKRDPENAR
                     LWMRRHLQDWRRGFEHIGKDLDQPLDRLYLQSIAPAEDER"
     gene            2255..3199
                     /locus_tag="VGP50_06370"
     CDS             2255..3199
                     /locus_tag="VGP50_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003618028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV8027035.1"
                     /translation="MDQRNLGKSGLRVSAVGLGCNNFGMKLDVEGTRKVVHRALDLGI
                     TFLDTADHYGNMGGSESCLGEVLGERRKDVVLATKFAMPMDAAGTKTGASRRYIMAAV
                     EASLTRLKTDWIDLYQLHRPDPLTPIEETLRALDDLVRQGKVRYVGCSNLRAWQVVEA
                     QWTAKHLGLNAFIACQDEYSLLVRDIEQDLVPAIEAYDLGLLPYFPLASGLLTGKYKR
                     NQTMPQGSRLALVKRLADRYLTESHWQAVERLQDFCDKRDHRLLDLAISWLLSRPRVA
                     SVIAGATTPEQVEQNIRAAEWKLTPEDVADVDRLSKAA"
     gene            complement(3248..4636)
                     /gene="creD"
                     /locus_tag="VGP50_06375"
     CDS             complement(3248..4636)
                     /gene="creD"
                     /locus_tag="VGP50_06375"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEV8027036.1"
                     /translation="METTATDSQPQPVLAAAPALVDAFSGPALKILLIVGLFLLMLIP
                     LSLVSSLIAERQERQGEVLKDFTTSWGPTQTVLGPLLVVPYRDVPDQSLRYLHVAPRQ
                     LEATVHLFPEIRKRGLFRAIVYSAKVDIKGTFQIPDEAVLRNFSTALRWQDSFILLRA
                     NDLRALASSAQLSWNGGALPWSDCAENGSTGCDGDRFVVARLALSGPPAADASIPFAT
                     SLDLRGTEAFWLAPLGKDVDMTASARWETPGFVGAVLPTANRVDSDGFSASWHVASNV
                     ATGRWLWSSASIVDTDESYRGKSHAEYRIGVELLEPVPTYRMVERSAKYGPLFLALSF
                     LTYFLFEAIAGVRIHIIQYGLLGLSLSLFALLLISLSEPLGFTGGYALSALMVLLQAS
                     IYTGSVGRAARPAAIFAAVLAALFSFLYVVLILEAYSLLVGALALFTALSITMAVTRH
                     VDWSKMRPSRPA"
     gene            4883..5569
                     /locus_tag="VGP50_06380"
     CDS             4883..5569
                     /locus_tag="VGP50_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8027037.1"
                     /translation="MARILIVDDDPAIREVVRFALDRAGFEIAQAGDGRSALEHFADV
                     APDLVVLDVLLPGLDGLDVCRELRRRSTVPILFLSSKDEEVDRIVGLEIGGDDYLVKP
                     FSPRELVARVKVLLRRAAASALPPAEGPVLRHGRLTLDLERARAAWDDREVALTATEF
                     AILRTLLRRPGKVYSRDNLMDGAYGVGKVVSDRTIDSHVRRLRAKFAALGAEPIETLH
                     GFGYKLSACA"
     gene            5569..7158
                     /locus_tag="VGP50_06385"
     CDS             5569..7158
                     /locus_tag="VGP50_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8027038.1"
                     /translation="MTRPRLRTVLLLINVVILILPLGGLWFLRLYESALVRQTESELV
                     AQAAVLAAAFKAERQQLLARGIAADDEAAPAAPAALPTARAAPQGLDLAEDPVLPPPP
                     DPAPSGAAPSPLALAAGTALLPVLRDVQPVTLASIRVTDRHGVIVATTGEDAGMSLAG
                     RQEIDGALKGDLVSLMRWRERPARPSVTGLSRGASLRVFVAMPVIDGDKVLGAVMLAR
                     TPRDLLQAIYGKRYPLIALAFLLLGAVTALAIIASRLITRPLGQVVAQAQQVAGGSLG
                     AVVPLSGPAIREAAELSAAVSRMAGTLERRADYIRSFAAHVSHEFKTPIASAKGALEL
                     LNDHFATMSVDERLRFLGVVAGSIDRLERLVRRLLDLARADMMRPTQAERTALAPLLE
                     RLTERYGRRGLTLRLRQGAAEVALAEDALDIVLSSLLDNVLDHAGRGSEVTIETNAAA
                     DGVTVTVADNGPGVSPEDAQRIFEPFFTTARASGGTGLGLSIARAVIAGAGGTMALVP
                     AARGACFRLTLPEGRSAAIRS"
     gene            complement(7297..8586)
                     /locus_tag="VGP50_06390"
     CDS             complement(7297..8586)
                     /locus_tag="VGP50_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8027039.1"
                     /translation="MEAQVLAKVNRRLVPFLILCYFVAYLDRVNVGFAALTMNKDLGF
                     SATMFGWGAGIFFIGYFIFEVPSNVALERFGARRWIARIMLSWGVLSTAMALIWNDVS
                     FLILRFLFGVAEAGFFPGIILFLTFWFPATHRARIVGNFMAAIPVSSVIGAPLSGWVL
                     GLNGVLGLAGWQWIFIIEGVPAILLSAIVLLYLTDGPAQASWLAPEERDWLIGRLAQE
                     RREREAIVHFTFWKALASPRVLLLGLVYFGIVAANYGTSFWLPQIVKGFGITNLETGF
                     VTAIPYLAGTVAMVWWGRRSDRARERKWHCAAPILIAAAGLALATTAGTPSLEMAALT
                     FAAVGIFAALPCFWTLPTALLTGTAAAGGIALINSIGNLAGFVGPYGMGYIKDATGSF
                     GAGLLFIAALAFASFLLVLALGHDPKLEASSARQPAE"
     gene            complement(8766..9383)
                     /locus_tag="VGP50_06395"
     CDS             complement(8766..9383)
                     /locus_tag="VGP50_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV8027040.1"
                     /translation="MDGDMTQRQRIREIVENWVLWRDQGDWTRFRTVWHQDGWMMATW
                     FQGPAERFIEVSREGWGRGVSILHFLGGSTIDVAGARAIAQTKMQILQRAAVDGVLSD
                     VTCTGRFYDFFEERQGRWGVVLRQPIYEKDRLDPVDPAAQLVLDPALLASFPEGYRHL
                     AYLQARNGFTVKRDMPGLKGPEVERLYARGAQWLQGKPIDPAWRA"
     gene            complement(9395..>9940)
                     /locus_tag="VGP50_06400"
     CDS             complement(9395..>9940)
                     /locus_tag="VGP50_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8027041.1"
                     /translation="GLGLSQVYGFAKQSGGDAIVRSEERCGTTVTLFLPRATRAPSVT
                     TDVAGSDVAPPHDGEKATVLLVEDNADVAEVSAALLGGLGYTVRVATSAQHALDRLAA
                     GETADLVFSDVVMPGGMNGLDLARTLRTRYPGLPVLLATGFGTEQQVMQSGFPLLLKP
                     YRAKALASAIESLLHAGRCVS"
BASE COUNT         1719 a         3238 c         3410 g         1573 t
ORIGIN      
        1 gccgaccgct ggattgaggt cgaaggcgag ggcgacgaag agacggcctt cgtcatggtt
       61 ggtgccctcg atcacgggca cgtggttgta ttcgccgagc gcgatcgccg taatcggctc
      121 ctgcgggatc accacgccat cgacatccgg gaagaacgcg aagagcgacg cgaggctgtt
      181 ggcgccggtg acggcgttct gcacagcgag gatctgcgcg atcgagaggc cgcgaaggca
      241 cgcgacggtc gcggcggccg tggcatcggc gcagtcgagc gcgctgacga tctgagcccc
      301 gcccgcctct gcggcggcct tggtcgggag tggtgtggcg caagggccgc tttcggtgat
      361 cgccgcctgg aacaagccct tggcagcggg cgagacgatg ttggcgcaga cgctgaagcc
      421 gccggcggac tcgccgaaaa tcgtcacgtt tcgcgggttg ccgccaaaag cggagatgtt
      481 ctgctgcacc catttgagcg cgaactgctg gtcgcggagc ccgtaattgc cggaggcgtt
      541 gttgggcgac acggcgtcga gcgcggcgtc ggccagataa cccaggatgc ccaagcggta
      601 attgatcgtg acgacgatcg tgtcgccctc catcgccatc tgggtcggat tgtagaggct
      661 gccctgaccg aacacgaagg cgccgccatg atagaagacc atgacgggca ggctgttgcg
      721 cgccgagaca gaggatcccg gaacgtagac attgaggaag aggcagtcct catcgttgct
      781 cgccgtgccg aaaggcgagg cggattgcgg gcaggggctg ccgaattgcg tggcctcgac
      841 cgtgccggtc accgctgcgg gaggcgtcgg cgccgcgaag cgcagattgc cggtcggcgc
      901 cgccgcatag cggataccga ggaaagtggt gatgccgttg ccggcgacgc cttggaatgt
      961 ctcggccggg aaagtcccgc caggggtggt cacggtttgc gcatcggcgc gtcccatcga
     1021 ggcgaccgcc gccgccatgg tcagcccgac cagggctgtt cgaagcaatg ccaaaggtga
     1081 ctttcggtcc gtcgtcatgc tgttccctcc gtttgttaaa cgtcattcgt cctctgccac
     1141 acttttccgg tgtttctctg gcctcgaatg ggtggtattc tgcccgaaaa tgtacggaaa
     1201 tcctggactg ttctaccttt agtagattcg gcccggattt ccggtacaac gtcgcattat
     1261 cctaaaaccg ctccgattgt ctgacaatcc gataagtcgg cgaacggacg atcaccggaa
     1321 cgttttgcga ctggagcttc gcatcaggca cggatcgtgc tagcggtggg tgtaggcaag
     1381 ggcgtgaggg cagagtcgat gtcggatacc gcagagagat tcgagcggat caacgcggcg
     1441 ccggcctatc agctggtggc cgaggcgatc gagaagggca tcaccagcgg ccggctgcgt
     1501 gccggcgaac cgctcggcac ggaagcggaa ctggtgcgac agttcggggt caaccgatcg
     1561 acggttcgcg aaggcatccg gatgctcgag caggccgggc tcgtgcaccg cgattccagc
     1621 cgccggctga ccgtcggcgt cccgcattat gaccggctcg cctcgcgcag gacgcgggcg
     1681 ctggtgatgc atgaggtgac cttccgcgag ttgagcgagg ccgtgctggt gctcgacctc
     1741 gccgcggtcg agcaggcggc gctccgcgcg acaccggccg tgatcgccga gctcgacgcg
     1801 aacatcgcca agaccgaagc ggcggccggc aactggccag tcgtcaccga gctcgatacc
     1861 cagtggcacg agctgttcta caaggcggcg ggcaaccgcg tgctccagct ggcgcgcgag
     1921 ccggcgccgc tgctcgccct ctcggtcacc cagatgatcc tcgagcaggt caaagaggcg
     1981 ttgccgcgtc tcctcgtcgc gcatcgcacc atcaccgagg cggtgcgcaa gcgcgatccg
     2041 gagaatgccc ggctgtggat gcggcggcac ctccaggatt ggcgccgcgg cttcgaacac
     2101 atcggcaagg atctcgatca gccgctcgat cggctctatc tgcaatcgat cgcgccggcg
     2161 gaagacgagc ggtagggtct tcgcccctca ctctcccaca aggggacagg gacgctaaga
     2221 tggacgcttg cgagaaatct tcggggaggt cgcggtggat cagcgcaatc tcgggaagtc
     2281 cgggttgaga gtgtcggcgg tcgggctcgg ctgcaacaat ttcggcatga agctcgatgt
     2341 cgaggggacg cggaaggtcg tgcaccgggc gcttgacctc ggcatcacct tcctcgatac
     2401 cgccgaccat tacggcaaca tggggggatc cgagagttgt ctcggcgagg tcctgggcga
     2461 gcgccgcaag gacgtcgtgc tggcgacgaa atttgccatg ccgatggacg ccgccgggac
     2521 caagaccgga gcatcccggc gctacatcat ggcggcggtc gaggcgagcc tgacgcggct
     2581 caagacggat tggatcgacc tctaccagct gcatcgcccg gatccgctga cgccgatcga
     2641 agagacgctg cgcgcgctcg acgacctcgt ccgccaaggc aaggtccgct atgtcggctg
     2701 ctccaacctc agggcgtggc aggtggtcga ggcgcaatgg acggccaagc atctgggcct
     2761 caatgccttc atcgcgtgcc aggacgagta cagccttctg gtgcgcgaca tcgagcagga
     2821 cctggtgccg gcgatcgagg cctatgatct cgggctgctg ccgtattttc cgctggcgag
     2881 cggattgctg accggcaagt acaagcgcaa tcagaccatg ccgcaaggct cgcgcctcgc
     2941 tctggtgaag cggctggccg accgctattt gaccgagagc cattggcagg ccgtcgagcg
     3001 gctccaggat ttctgcgaca agcgcgacca tcgcctgctc gatctcgcca tctcctggct
     3061 cttgtcgcgc ccgcgcgtcg ccagcgtcat cgccggcgcc accacgcccg agcaggtcga
     3121 gcagaacatc cgcgcggctg aatggaagct cacgcctgag gacgtcgccg acgtcgatcg
     3181 cctgagcaaa gcggcctgac ggcaatggtc gtcagaaggg ggcgtcaggt cgcggcctcg
     3241 cgtggcgtta tgctggccgg ctgggccgca tcttcgacca gtccacatgg cgcgttactg
     3301 ccatggtgat cgagagggcc gtgaagagcg cgagcgcgcc gacgagaagc gaataggcct
     3361 cgaggatcaa gacgacataa aggaagctga agagcgcggc cagaacggcg gcgaagatcg
     3421 ccgcgggccg cgcggctcga ccgacgcttc cggtatagat cgaagcctga agcaaaacca
     3481 tgagcgcgct cagcgcatag ccgccggtga agcccagcgg ctcgctcagc gagatcagca
     3541 gcagcgcgaa aagcgagagg gaaagcccga ggagaccgta ttgaatgatg tggatacgca
     3601 ccccggcaat cgcctcgaag agaaaatagg tgagaaacga cagcgcgagg aaaagcgggc
     3661 catatttggc ggagcgctcg accatccggt aggtcggcac cggttccagc agctctacgc
     3721 cgatacggta ctcggcatgg ctcttgccgc ggtagctttc gtcggtgtcg acgatcgacg
     3781 ccgacgacca gagccagcgg cccgtcgcca cgttgctggc aacatgccac gatgccgaga
     3841 aaccgtccga gtcgacacga ttcgcggtcg gcaggacggc gccgacgaag cccggggtct
     3901 cccagcgtgc gctcgccgtc atgtccacgt ccttgcccag cggcgcgagc cagaaggcct
     3961 cggtgccacg gaggtcgagg ctggtggcga aggggatgga ggcatccgcc gcgggcggcc
     4021 cgctgagcgc gagccgcgcc accacgaagc ggtcgccgtc gcagccggtg ctgccgttct
     4081 cggcgcaatc agaccaggga agcgcgccgc cattccagct gagctgcgcc gaggaggcga
     4141 gggcgcgcag gtcgttcgcg cgaaggagga tgaagctgtc ctgccagcgc agcgccgtcg
     4201 agaagtttcg cagcaccgcc tcgtctggaa tctggaacgt gccctttata tcgaccttgg
     4261 cgctgtagac gatggcgcgg aagaggccgc gcttgcggat ctccgggaac agatggaccg
     4321 tcgcctcgag ctggcgcggc gccacgtgaa gataacgcag cgactggtcc gggacatcgc
     4381 ggtaaggcac caccagcaag ggaccgagaa cggtctgtgt cggtccccag ctcgtcgtga
     4441 agtccttgag cacttcgcct tggcgttcct ggcgctcggc gattagcgaa gacaccagcg
     4501 agagcgggat cagcatcagc aggaacaggc cgacgatcag caggattttg agagcgggcc
     4561 cgctgaaggc gtcgacgaga gccggggccg ctgccagcac cggctgcggc tgtgaatcgg
     4621 tcgcggtcgt ttccatgaag ctcctccgat gagtccggct cgatgctggc gctacttcat
     4681 gccccggccg tggcgaggac atggaaatcg cccgtcattc tcgtgccgat ttggtgcaga
     4741 tgaggaccct tctcctcgtg cctggacgac ggcgccattc tcacggggcg agatgccgcg
     4801 gcgatggcgc cgccgcggaa aatccttgcc tttcccgtag cgatttcgtg caaatgaccg
     4861 aggcgacgga gatcggcgaa gaatggcgcg catcctgatt gtcgatgacg atccggcgat
     4921 ccgcgaggtc gtgcggtttg cgctcgaccg cgccggtttc gagatcgccc aggccgggga
     4981 cggacgatcg gcgctcgagc atttcgccga cgtagcgccc gatctcgtcg tactcgatgt
     5041 gctgctgccc ggacttgacg gactcgatgt gtgccgcgag ctgcggcgcc gcagcaccgt
     5101 gccgatcctg ttcctctcgt ccaaggacga ggaagtcgac cgcatcgtcg ggctcgagat
     5161 cggtggtgac gattacctcg tgaagccgtt cagcccccgc gaactggtcg cgcgcgtcaa
     5221 ggtattgctg cgccgcgccg cggcgtccgc actcccgccg gccgaaggcc ccgtgctgcg
     5281 gcacggacgc cttacgctcg acctcgagcg cgctcgcgcc gcctgggacg accgcgaggt
     5341 cgcgctcacc gccacagagt tcgccatcct gcgcacgctg ctgcgccggc ccggcaaggt
     5401 ctattcgcgc gacaacctca tggacggcgc ctatggcgtc ggcaaggtcg tgagcgaccg
     5461 caccatcgac agccatgtcc gccgcctgag agccaaattc gccgcgctcg gcgccgagcc
     5521 catcgagacg ctgcatggct tcggctacaa gctctcggcc tgcgcctgat gacgcggccg
     5581 cggctcagga cggtgctgct gctgatcaat gtggtgatcc tcatcctgcc gctcggcggt
     5641 ctctggttcc tgcgcctcta tgagagcgcg ctcgtccgcc agacggaatc ggagctggtg
     5701 gcccaagcgg cagtgctcgc cgcggcgttc aaggcggagc gccagcagct tctcgcgcgc
     5761 ggcatcgccg ccgatgacga ggccgcgcct gccgcgcctg ccgcgctgcc gacggcgcgc
     5821 gccgctccgc aagggctcga tcttgccgag gacccggtgc tgccgccacc acccgatccg
     5881 gcaccgtccg gcgcggcccc aagcccgctc gcgcttgccg ccggcacggc gctcctgccg
     5941 gtgctgcgcg acgttcagcc ggtgactctg gcatcgatcc gcgtcaccga tcggcacggc
     6001 gtcatcgtcg cgacgacggg ggaggacgca ggaatgagcc tcgccgggcg ccaggagatc
     6061 gacggcgccc tcaaaggcga tctggtcagc ttgatgcgct ggcgcgagcg cccggcgcgg
     6121 ccgtcggtga cggggttgag ccggggcgcc agtcttcgcg tcttcgttgc catgcccgtg
     6181 atcgacggcg acaaggtgct gggcgcggtc atgctggcgc gcacgccgcg cgacctgttg
     6241 caagcgatct acggcaagcg ctacccgctg atcgccttgg cgttcctgct gctcggcgcg
     6301 gtcacggcgc tcgccataat cgcctcgcgc ctcatcacgc gccctttggg ccaggtcgtc
     6361 gcccaagcgc agcaggtcgc cggcggttct ctgggagccg tcgtgccctt gagcgggccg
     6421 gcgattcgcg aagccgccga gctttcggcg gccgtctcgc gcatggcagg gactctggag
     6481 cggcgtgccg actatatccg cagcttcgcc gcacatgttt cgcatgagtt caagacgccg
     6541 atcgccagcg ccaagggtgc gctcgagctt ctcaacgatc atttcgcgac gatgtcggtg
     6601 gacgagcgcc tccgtttcct cggcgtcgtc gccggcagca tcgaccggct cgagcggctg
     6661 gtgcgccgcc tgctggatct cgcgcgtgcc gacatgatgc ggccgacaca ggccgagcgc
     6721 acggcgctgg cgccgctctt ggagcggctc accgagcgct acggccggcg cggccttacg
     6781 ctgcgcctgc gtcaaggcgc ggcggaggtc gctctcgcgg aggatgcgct cgatatcgtg
     6841 ctctcttcgc tgctcgacaa cgtcctcgac catgcgggac gcgggagcga agtgacgatc
     6901 gagaccaacg ctgcggcgga tggcgtcacg gtaaccgttg ccgacaacgg cccgggtgtg
     6961 tcgccggaag atgcccagcg catcttcgaa ccctttttta cgacggcgcg cgcgagcggc
     7021 ggcaccggcc tcggtctctc catcgcgcgc gcggtcatcg cgggcgctgg tggcacgatg
     7081 gcgctggtac cggccgcccg cggcgcgtgc ttccgtctca cccttcccga gggccggtcg
     7141 gcggcgatcc gctcatgagt cccggccgga cgatcggcag ctatctcggc gccaatgccg
     7201 gacgcccgcg ctccgagcgc tgcggtccgc cgcgggcggc gagcgccatc gtcagccgcg
     7261 tgtgtctgac ggggccgagg acccgcaacg gcaatgctat tccgccggct ggcgtgcgct
     7321 gctcgcttcg agcttggggt cgtggcctaa agcgaggacg agcaggaagg aggcaaaggc
     7381 gagggcggcg atgaacagca agccggcgcc gaagctgccg gtcgcgtcct tgatgtagcc
     7441 catgccatag ggcccgacga agccggcgag gttgccgatc gagttgatga gggcgatgcc
     7501 gccggcggct gccgtgccgg tgagaagggc cgtgggcagc gtccagaagc aggggagcgc
     7561 ggcgaagatg ccgacggccg cgaacgtcag cgccgccatc tcgagcgacg gcgtgccggc
     7621 cgtggtcgcc agcgcgaggc ccgccgcggc gatgaggatc ggcgccgcgc aatgccattt
     7681 ccgctcgcgc gcccggtcgg agcgccggcc ccaccacacc atggcgacgg tgccggcgag
     7741 ataggggatc gccgtgacga agccggtctc gaggttggtg atgccgaagc ccttgacgat
     7801 ctgcggcagc cagaagctcg tgccgtaatt ggcggcgacg atgccgaaat agacgagacc
     7861 gagcagcagc acgcgcggac tcgccagcgc cttccagaag gtgaaatgga cgatcgcctc
     7921 gcgctcccga cgctcttgcg cgagacggcc gatgagccag tcgcgctctt cgggcgcgag
     7981 ccatgaggcc tgggccgggc cgtccgtcag atagagcagc acgatggcgg agagcaggat
     8041 cgcgggcacg ccctcgatga tgaagatcca ttgccagccg gcgaggccaa ggacgccgtt
     8101 caggccgagg acccagccgg acaacggcgc gccgatcacc gaggagaccg ggatcgcggc
     8161 catgaaatta ccgacgatcc gcgcgcggtg ggtggcggga aaccagaatg tcaggaacag
     8221 gatgatgccg ggaaagaacc cggcctcggc gacgccgaag agaaagcgca ggatgaggaa
     8281 gctgacgtcg ttccagatca gcgccatggc cgtcgacagc acaccccagc tcagcatgat
     8341 ccgcgcaatc cagcggcgcg cgccgaagcg ctcgagcgcg acattgctcg gcacctcaaa
     8401 gatgaagtag ccgatgaaga aaatgccggc gccccagccg aacatggtgg cgctgaatcc
     8461 cagatccttg ttcatcgtca gcgcggcaaa gccgacatta acgcgatcga gataggccac
     8521 gaagtagcag aggatcagga acggcacgag ccgccgattg accttcgcga ggacctgcgc
     8581 ttccaccccg gttcctccat tttatgcgcc aatgatcgca ccggagtcgg agcaagacaa
     8641 ggttcaccac agagacacag agacacagag acacagagac acagaggatc aggccgtcgg
     8701 gtgcgcgacg cgcacccttt tttcttctct gtgcctctgt gtctctgtgg tcgatcttcc
     8761 ctctttcagg cgcgccaagc ggggtcgatc ggttttcctt ggagccattg cgcgccgcgc
     8821 gcatagaggc gctcgacctc cgggcccttg aggccgggca tgtcgcgttt caccgtgaag
     8881 ccgttccggg cctggagata ggccagatgg cggtagccct cgggaaagga cgcgagcagc
     8941 gccgggtcga ggacgagttg cgccgccgga tcgacgggat cgaggcggtc cttctcgtag
     9001 atcggctggc gcagcaccac gccccagcgg ccttgccgct cctcgaagaa atcatagaag
     9061 cggccagtgc aggtaacgtc cgagagcacg ccgtcgaccg cggcgcgctg caggatctgc
     9121 atcttggtct gagcgatggc gcgcgcgcca gcaacatcga tggtgctgcc gccgaggaaa
     9181 tgcaggatgc tgacaccgcg gccccagccc tcgcggctga cctcgatgaa gcgctcggcc
     9241 gggccctgga accaggtggc catcatccag ccatcctgat gccacaccgt gcggaagcgg
     9301 gtccagtcgc cctggtcgcg ccacagcacc caattctcga cgatttcgcg gatgcgctgg
     9361 cgttgcgtca tgtcgccgtc catcgcgccc tctctcaaga gacgcagcgg ccggcatgca
     9421 gcagggactc gatcgcgctc gcgagcgcct tcgcccgata aggcttcagc agcagcggga
     9481 agccgctttg catcacttgc tgctccgtgc caaagccggt tgcgagcagc accggaagcc
     9541 ccggatagcg cgtccggagc gtgcgcgcca gatcgaggcc gttcatgcca ccgggcatca
     9601 ccacgtccga gaagacgagg tcggcggtct cgccggcggc gagcctgtcg agcgcgtgct
     9661 gtgccgaggt cgcgacgcgc acggtataac cgaggccgcc cagcagcgcg gcacttacct
     9721 cggcgacatc ggcattgtcc tcgaccagca gcaccgtcgc tttctcgccg tcatggggcg
     9781 gcgccacgtc gcttccggcg acatcggtcg tcaccgacgg cgcgcgcgtg gcccgcggca
     9841 ggaacagtgt caccgtcgtg ccgcacctct cttcgctgcg cacgatggca tcgccgcccg
     9901 attgcttggc gaagccatag acctggctca gacccagccc
//
LOCUS       DASYNS010000207         3081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4414866, whole genome shotgun sequence.
ACCESSION   DASYNS010000207 DASYNS010000000
VERSION     DASYNS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4414866"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VGP50_06405"
     CDS             complement(<1..764)
                     /locus_tag="VGP50_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV8027042.1"
                     /translation="MISPSERMIAEKDGAIGWMVFNNPGRRNAISLDMWQAVPAILDA
                     FEQDAEIRVIVLKGAGDQAFISGADISQFEQQRSSAETVAHYDKIAETAGARLQETTK
                     PTIAMIHGFCIGGGLGVALQCDLRIAADNARFAVPAAKLGLGYRWGGVKKLVDTVGPA
                     YAKEIFFTARQFDAGEALAMGLVNRVLPAAELEAYVRRYCALMAENAPLTIEAVKGVI
                     QELHKPGAEIDRRRCEALVARCFESQDYIEGRRAFM"
     gene            complement(778..1950)
                     /locus_tag="VGP50_06410"
     CDS             complement(778..1950)
                     /locus_tag="VGP50_06410"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV8027043.1"
                     /translation="MSAPPLSRFKVVDLTRVRAGPTAVRQLADWGAQIIKVEAVEGDG
                     GLGGARHGPDFQNLHRNKRSITLDLKKPEGLDVLHRLVTDADVLVENYRPDVKHRLKV
                     DYESLGAINPRLVYASISGFGQDGPYHDRPGFDQIAQGMGGLMSITGLPGQGPVRVGI
                     PVADLSAGIFCAMGILVALLEREVSGQGQWVQTSLLQAQIAMLDFQAARWLIAGEIPG
                     QAGNNHPTSIPTGVFKTADGHINIAAAGQEMFRRLAGALGAPRLAAEPDYADDKHRLK
                     NRDKLNAAIEEITKTKSSADWIEILNRAGVPSGPIYRMNEVFADPQVKHLGIAQAVEH
                     PTLGHLELVGQAVTLSRTPSKLETASPERGAHTDTVLRELGYDADAIAGFRAKGIV"
     regulatory      2135..2210
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2223..2933
                     /locus_tag="VGP50_06415"
     CDS             2223..2933
                     /locus_tag="VGP50_06415"
                     /inference="COORDINATES: protein motif:HMM:NF017815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEV8027044.1"
                     /translation="MRIFRSLALVVAALGLLSRWAAAEDARMAPAGEAAAHPAIFGAR
                     EIYSPDLSAFVKWTGMLERFAREEREEPPCAPGSGRDCLPGEWRELLGALDGLDLRTK
                     LDRVNAAINGHPYVASMRNWGESNHWETPFEFMRRGGQCQDYAIAKYLLLRAAGVPAA
                     RLRIVVLYDRRLGLDHAVVVAYVEGRVLMLDNQLASVVPADSVDHYQPYYSINEEGWW
                     LHLGPNARYAAADFGGRD"
BASE COUNT          492 a         1012 c         1036 g          541 t
ORIGIN      
        1 tccatgaagg cgcggcggcc ttcgatgtag tcctggctct cgaagcagcg ggcgaccagc
       61 gcctcgcagc gccgacggtc gatctcggcg ccgggcttgt gcagctcctg gatcacgccc
      121 ttcaccgcct cgatggtgag cggcgcgttc tcggccatga gggcgcagta gcgccgcaca
      181 taggcctcga gctcggccgc cggcagcacg cggttgacga ggcccatggc gagcgcctcg
      241 ccggcgtcga actggcgggc ggtaaagaat atctccttgg cgtaagcggg tccgacggtg
      301 tcgacgagct tcttgacgcc gccccagcga tagccgagcc cgagctttgc cgcggggacg
      361 gcgaagcgcg cgttgtcggc ggcgatgcgc agatcgcatt ggagcgcaac gccgaggccg
      421 ccgccgatgc agaagccatg gatcatggcg atcgtcggct tcgtcgtctc ctgcaggcgg
      481 gcgcccgccg tctccgcgat cttgtcgtag tgcgcgaccg tttcggccga ggagcgctgc
      541 tgctcgaatt gcgagatgtc ggcgccggag atgaaggcct gatcgcccgc ccccttcagc
      601 acgatgacgc ggatctcggc atcctgctcg aaggcgtcga ggatcgccgg aacggcctgc
      661 cacatgtcga gcgaaatggc gtttcgccgc cccgggttgt tgaacaccat ccagccgatc
      721 gcgccgtcct tctcggcaat catgcgctcg ctcggagaaa tcatcgcctc atcctcttca
      781 tacgatgccc ttggcgcgaa agccggcgat ggcgtcggca tcgtagccga gctcgcgcag
      841 cacggtgtcg gtgtgcgcgc cgcgctcggg cgacgctgtt tcgagcttcg agggcgtgcg
      901 gctcaacgtc acggcctgtc ccaccagctc caggtggccc agcgtcggat gctcgaccgc
      961 ctgcgcgatt ccgagatgct tgacctgggg gtcggcgaag acctcgttca tgcggtagat
     1021 cggtccgctc ggcaccccgg cgcggttgag gatctcgatc cagtccgccg agctcttggt
     1081 cttggtgatc tcctcgatcg cggcattgag cttgtcgcga ttcttgagcc ggtgcttgtc
     1141 gtcggcgtaa tcgggctcgg cggcgagacg cggcgcgccc agcgcgcccg ccaggcggcg
     1201 gaacatctcc tggcccgcgg cggcgatgtt gatgtggccg tcggcggtct tgaagacgcc
     1261 ggtcggaatg ctggtcggat gattgttgcc ggcctggccg gggatctcgc cggcgatcag
     1321 ccagcgcgcc gcctggaaat cgagcatggc gatctgcgcc tggagcagcg acgtctgtac
     1381 ccattgccct tgccccgaca cctcgcgctc cagcagcgcg accaggatgc ccatggcgca
     1441 gaagatgccg gccgagaggt cggccacggg aatgccgacg cgcaccgggc cctggccggg
     1501 caggccggtg atcgacatga gcccgcccat gccttgggcg atctggtcga aaccgggacg
     1561 atcgtggtaa ggcccgtcct ggccgaagcc ggagatgctg gcatagacga ggcgcgggtt
     1621 gattgccccg aggctctcgt aatcgacctt cagacggtgc ttgacgtcgg gacggtagtt
     1681 ttcgaccagc acgtcggcgt ccgtgacgag ccgatgaagg acgtcgaggc cctcgggctt
     1741 cttgagatcg agggtgatgc tgcgcttgtt gcgatggagg ttctggaaat cgggcccgtg
     1801 tcgcgcgccg cccaagccac catcgccctc gaccgcttcg accttgatga tctgtgcacc
     1861 ccagtcggcg agctggcgca cggctgtcgg gccggcgcgg acgcgggtca gatcgacgac
     1921 tttgaaccgg gaaaggggag gggcgctcat gattcccatg ccgccgcaac acgagctgcg
     1981 gctgatcgcc ggccaatatc ggccttccgc gcaaacgggg caagcctccg ccggccggcg
     2041 tagaaattct aataatcaca agcagttacc taagacaagg ccgagccgtc ccattcaccg
     2101 gacattaagc ggaagcgtgg ttcaattgcg ctgtatcacg aaacggcaaa accggggcga
     2161 cccggcgacg caaagccacc ggtctctctg gagacagcgg ggctaccgaa tgaggccccg
     2221 ccttgcggat tttccgttcc ctggcactgg tcgtcgccgc gctcggcctg ctgtcgcgct
     2281 gggccgccgc agaagacgcc cgcatggcgc cggctgggga ggccgctgct catccggcga
     2341 tcttcggggc ccgcgagatc tactcgcccg acctttcggc tttcgtcaaa tggaccggca
     2401 tgctcgagcg cttcgcacgt gaggaacggg aagagcctcc ctgcgctccg ggttccggtc
     2461 gggactgctt gcccggcgaa tggcgagagc tcctgggcgc gctcgacggt ctcgacctca
     2521 ggaccaagct cgaccgggtc aacgccgcga tcaatggcca cccctatgtc gcctcgatgc
     2581 gcaattgggg cgaaagcaat cactgggaga cgccgttcga gttcatgcgc cgcggcggcc
     2641 agtgccagga ctacgccatc gccaaatatc tcctgctccg cgccgcggga gtcccggcgg
     2701 cgcgactacg gatcgtcgtg ctgtacgata ggcgcctcgg cctcgatcat gccgtcgtgg
     2761 tcgcctatgt cgaggggcgc gtgctcatgc tcgacaacca gctcgccagc gtcgtgccgg
     2821 cggacagcgt cgatcactac cagccctatt actcgatcaa cgaagagggc tggtggcttc
     2881 atctcgggcc caatgcccgc tatgccgcgg ccgacttcgg cggccgcgac tgagcgcccg
     2941 cccatagcgg gttggccgat tctcgacttc gtaactccga gcgcgtagga tcgtctgccc
     3001 ctccgggggg aggaaacaag aagatggatg tgcaacgcct cagcgtagaa tatggcgcgt
     3061 ggttctatct cctgaccttc g
//
LOCUS       DASYNS010000208         7545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4725646, whole genome shotgun sequence.
ACCESSION   DASYNS010000208 DASYNS010000000
VERSION     DASYNS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7545
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4725646"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VGP50_06420"
     CDS             complement(<1..600)
                     /locus_tag="VGP50_06420"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEV8027045.1"
                     /translation="MSERSRVVVIAEAGVNHNGSLDRARALVDAAVAAGADIVKFQTF
                     RADRIATRAAAKAEYQKSDGAAESQHAMLERLELDEAAHAVLVPYCAERGIEFLSTPF
                     DLPSVDLLVRRHALARLKISSGDLTNGPLLLHAARSGAALILSTGMADLGEVEAALGV
                     VAFGLTDPAALPGEAAFEAAYASAAGQQALHARVTLLHCT"
     gene            complement(602..1762)
                     /gene="neuC"
                     /locus_tag="VGP50_06425"
     CDS             complement(602..1762)
                     /gene="neuC"
                     /locus_tag="VGP50_06425"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEV8027046.1"
                     /translation="MTRRRVCVVSGGRADYGLLYWPMKEIAASAEFELQLVLTGMHLA
                     PEFGATAAQAERDGFTVAARVEMLLASDTAVGVAKSIGLGVIGFADAFERLKPDLLIL
                     LGDRFEILAAATAALVARLPVAHLCGGDVTAGAFDEAIRHAITKMAHLHFATHAEAAR
                     RLRQLGEDPQRIFNVGSPGVDYIRRLEPMTRDDVARETGLDLGGRVLLVTFHPPTLGS
                     TPALVEFEELLAALDAQGPETTLAFTMPNADTAGRALAARLERFVECHAKARLFRALG
                     SRLYLNLLRQADAIIGNSSSGLLEAPSFGTPTVDIGERQQGRPRAESVISCRAERRAI
                     AAAIAAARQRPKAPVANPYGAGDAARRIVETLRAIGDFRALTQKRFFDLPGA"
     gene            complement(1899..3107)
                     /locus_tag="VGP50_06430"
     CDS             complement(1899..3107)
                     /locus_tag="VGP50_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HEV8027047.1"
                     /translation="MTSVAQRKPETGLDIDRVIAAIRAALPDGIATAPLHKPCFDGNE
                     WTYLKECLDSSYVSSVGSFVPRFEKQVAEAAGTAHAVAVMNGTAALHVCLLLAGVARG
                     DEVLAPALTFVATANAISYCGAVPHFVDSDLRTLGVDPRRLGEYLRRSTVRRGDDLVN
                     PRTGRRIKALMPMHSFGHPVDMEPLLALAAEFGLFVIEDAAESLGSRYKGQPTGGLAR
                     LGAFSFNGNKIVTTGGGGAITTDDAELAQAAKHLTTTAKLPHPWAFIHDRVGYNYRMP
                     NLNAAVGCAQLEQLPDFLAAKRALAERYRGALADVPGVSFVVEPPFARSNYWLNALLL
                     DAGHAAERDALLARSNAEGLATRPVWTLMHHLPMYADCPRMELPVAEDIARRLVNLPS
                     GAGLAPRREG"
     gene            complement(3104..3517)
                     /locus_tag="VGP50_06435"
     CDS             complement(3104..3517)
                     /locus_tag="VGP50_06435"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEV8027048.1"
                     /translation="MEKGRSSPEAKPYEFAHLSFVRDTATGLIAALEAGDAATGEVIN
                     LGSGFEIAIGDLAREIAEIAGAPLAIECDQQRLRPAKSEVERLFSDNGKAARLLGWRP
                     ERGGVDGLRRGLKETIDWFRVPENLALYKAKAYNR"
     gene            3702..4583
                     /locus_tag="VGP50_06440"
     CDS             3702..4583
                     /locus_tag="VGP50_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027049.1"
                     /translation="MNTLAQIAAAGAQTQSQEQLSIIGARMTAQLNQKIAELKAQGND
                     PMIPILQQQSATLSQQQTSYATAQSQLVANGGPIDDLDTQLSTMATAATAGDSATFDQ
                     ALSTANTDLADLNSGPLLPGFQPDGSANLKTNGLGIQSSAGYDLSTPAGQAQAAADIS
                     AAQTAVSQLSTVTAQNTQIAISVDQALATQIGALDDQVASRETATTGNAATQIQTLQT
                     QTQTQFHLIELSMGSASNTGSMLQDFETAQAAAAGPGSILNLLDTQVPSTSSSTSNSS
                     SKANSSSGTSSNFSTLA"
     gene            complement(4860..5282)
                     /locus_tag="VGP50_06445"
     CDS             complement(4860..5282)
                     /locus_tag="VGP50_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027050.1"
                     /translation="MALPSQFSLLHSDLNDFLFAQIGEEASGAPLSVLSALTRLGADP
                     WAEGARLSDLPRDVAARSLVPTIAMFPEATRSGSDVLKLAERLAALLPQRALDPATKL
                     IVRGGRWRIGLPRGRSLGIALLLTLLAVTAACWWLATQ"
     gene            complement(5427..7043)
                     /gene="groL"
                     /locus_tag="VGP50_06450"
     CDS             complement(5427..7043)
                     /gene="groL"
                     /locus_tag="VGP50_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387923.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV8027051.1"
                     /translation="MSAKDVKFSGDARDRMLRGVDILANAVKVTLGPKGRNVVLDKPY
                     GGPRITKDGVTVAKAIELPDRFENMGAQMVKEVAAQTSDLAGDGTTTATVLAQTIVRE
                     GAKAVAAGMNPMDLKRGIDMAVAAAVAEIKKHSKAVSTNAQIVQVGTISANGDREIGE
                     MIARAMDKVGKEGVITIEEAKSRETELEIVEGMQFDRGYLSGYFVTNAEKMLCELENP
                     YILLFDKKISGLQVMLPLLEAVAKAGKPLLMVAEEFEGEALATLVVNKLRGSLKVAAV
                     KAPGFGERRKAMLDDIAVLTGGQVVSDDLGIKLENVTLEMLGRAKKVQIDKDDTTIID
                     GGGAKKDIAARVAQLRAQVEEATSDYDKEKLQERLAKLAGGVAIIRVGGETDIEVKER
                     KDRVDDAMHATRAAAEEGIVAGGGLALLYAGQALARLKPGNDDQRVGIDVVRRALQAP
                     ARQILANAGADGSIILGKLLETGDVTYGFDAQTGTYVDMIKAGIIDPTKVVRLALQGA
                     ASIAGLLITTEAMVAEKSEKNAPPAAGGDF"
BASE COUNT         1223 a         2674 c         2442 g         1206 t
ORIGIN      
        1 cgtgcagtgc agcagtgtga cgcgcgcgtg cagcgcctgt tggcccgccg cgctcgcata
       61 ggctgcttcg aacgccgctt cgcccggcag cgccgccgga tccgtcaggc cgaacgcgac
      121 cacgcccagc gccgcttcca cctcgcccag atccgccatg cccgtcgaca ggatgagcgc
      181 cgcgccactg cgtgccgcat ggaggagcag cggcccgttg gtgaggtcgc ccgacgagat
      241 cttgagacgc gccagcgcat ggcgccgcac cagcagatcg acgctcggca ggtcgaaggg
      301 cgtcgacagg aactcgatgc cgcgctcggc gcaatagggc acgagcacgg cgtgggccgc
      361 ttcgtcgagc tcgagccgct ccagcatcgc atgctggctc tcggcggcgc cgtcgctctt
      421 ctgatactcg gccttggcgg ccgcacgcgt cgcgatgcgg tcggcgcgga aggtctggaa
      481 cttgacgata tcggcgccgg ctgccacggc cgcgtcgacg agcgcccgcg cgcggtcgag
      541 cgagccgtta tgattgaccc cggcctcggc aatgacgacg acgcggcttc tctcgctcat
      601 ctcaggcacc gggcaaatcg aagaagcgct tttgtgtcag cgcgcggaaa tcgccgatcg
      661 cgcgcagcgt ctcgacgata cggcgcgccg catcacccgc gccataggga ttggcgaccg
      721 gcgccttcgg ccgctggcgc gccgcggcga tggcggcggc gatggcgcgg cgctcagccc
      781 ggcacgagat caccgactcc gccctgggcc tgccctgctg gcgttcgccg atatcgaccg
      841 tgggcgtccc gaacgacggc gcctcgagaa gaccgctcga cgaattgccg atgatcgcgt
      901 ccgcctgccg cagcaggttc agataaagcc gcgagcccag cgcccggaag agtcgcgcct
      961 tggcatgaca ctcgacgaag cgctcgagcc gcgccgccag cgcgcgcccg gccgtgtcgg
     1021 cattgggcat cgtgaaagcg agcgtcgtct ccggcccttg cgcatcgagc gcggccagca
     1081 gctcttcgaa ttcgacgagc gccggcgtgc tgccgagcgt cggcggatgg aaggtcacga
     1141 gcagcacgcg gccgccgaga tcgagccccg tctcgcgcgc cacatcgtcg cgcgtcatcg
     1201 gctcgaggcg gcggatgtaa tcgacgccgg ggctgccgac attgaagatc cgttgcgggt
     1261 cctcgccgag ctggcgcagc cggcgcgccg cctcggcatg ggtggcgaaa tgcagatgcg
     1321 ccatcttcgt gatggcgtgg cggatcgcct cgtcgaacgc gcccgcggtg acgtcgccgc
     1381 cgcagagatg cgcgacgggc agccgcgcga ccagcgccgc cgtcgccgcc gccagaatct
     1441 cgaagcggtc gcccagcagg atcagcagat cgggcttgag ccgctcgaac gcatcggcga
     1501 agccgatgac gccgaggccg atcgacttcg ccacgcccac cgccgtgtcg ctggcgagca
     1561 gcatctcgac ccgcgccgcc acggtaaatc cgtcgcgctc cgcctgcgcc gctgtcgcgc
     1621 cgaactccgg cgccagatgc atcccggtga gcacgagttg cagctcgaat tccgccgaag
     1681 ccgcgatctc cttcatcggc cagtagagca acccataatc cgcccgcccg cccgacacca
     1741 cacagacccg ccgccgcgtc atcgtcgcca accccccacc cctccctccc cctcaagggg
     1801 ggagggggaa gacctgatgg ccgcaccagc gaatcccctc cccccttgag ggggagggtc
     1861 agggaggagg gtaagcccca ggctcgccat ttcccggact aaccctcgcg ccgcggcgcg
     1921 agcccggcgc cgctcggcag gttgacgagc cgccgcgcga tgtcctcggc caccggcagc
     1981 tccatgcgcg ggcagtccgc atacatcggc aggtgatgca tgagcgtcca taccgggcgc
     2041 gtggcgagcc cctcggcgtt ggagcgcgcg agcagcgcgt cgcgctcggc ggcatgaccg
     2101 gcatcgagca gcagcgcgtt gagccagtaa ttgctgcgcg cgaagggcgg ctcgacgacg
     2161 aaagaaacgc cgggaacgtc ggccagcgcg ccgcgatagc gctcggccag cgcgcgcttc
     2221 gccgcgagaa aatcgggaag ctgctcgagc tgcgcgcagc cgaccgcggc attgaggttg
     2281 ggcatgcgat agttatagcc gacacggtcg tggatgaagg cccagggatg cggcagcttc
     2341 gcggtggtgg tgagatgctt cgccgcctgc gccagctcgg cgtcatcggt ggtgatggca
     2401 ccgccgccgc ccgtggtgac gatcttgttg ccgttgaagc tgaaggcgcc gagccgcgcc
     2461 aagccgcccg tcggctgccc tttgtagcgg ctccccaggg attcggccgc gtcctcgatg
     2521 acgaagagcc cgaactccgc cgcgagcgcg agcagcggct ccatgtccac gggatggccg
     2581 aagctgtgca tcggcatcag cgccttgatg cggcgtcccg tgcgcgggtt cacgagatcg
     2641 tcgccgcggc gcaccgtgct gcggcgcaga tattcgccga gccgccgcgg gtcgacgccg
     2701 agcgtgcgca ggtcgctgtc gacgaaatgc ggcaccgcgc cgcaatagct gatggcgttg
     2761 gccgtcgcca cgaaagtgag tgccggcgcc agcacctcgt cgccccgcgc cactcccgcc
     2821 agcagcaggc acacatgcag cgccgccgtg ccgttcatga ccgccacggc atgtgcggtc
     2881 cccgccgcct cggcgacctg cttctcgaaa cgcgggacga agctgcccac cgacgacaca
     2941 tagctgctat cgaggcattc tttgagatac gtccattcat tcccgtcaaa gcacggctta
     3001 tgcagcggcg ccgtggcgat gccgtccggc agcgccgcgc ggatcgcggc gatgacgcgg
     3061 tcgatgtcga gccccgtctc gggctttcgc tgcgcgacgc tggtcatcgg ttgtaggcct
     3121 ttgccttgta gagcgcgaga ttctccggca cgcggaacca gtcgatggtc tctttgagtc
     3181 cgcggcgcaa gccgtcgacg ccgccacgct caggccgcca gccgagcagt cgcgccgcct
     3241 tgccgttgtc ggagaagagc cgctcgacct cgctctttgc cgggcggagg cgctgctgat
     3301 cgcattcgat ggcgagcggc gcgccggcaa tctctgcgat ctcccgcgcg agatcgccga
     3361 tcgcgatctc gaagccgctg ccgaggttga tcacctcgcc cgtcgccgcg tcacccgcct
     3421 cgagcgccgc gatgaggccc gtcgccgtgt cgcgcacgaa ggagagatga gcgaactcat
     3481 aaggttttgc ctcgggggag cttcggccct tctccatagc agcgtcttgg caaaggaacc
     3541 cttaacgggg ccgaaatatc cgctcgtttc aggggtctag aaggcggtcc ggagggtggc
     3601 aattcagctc tcctttaccc gtccgctcta ggatggatcg gttgcactct gcctttcggg
     3661 agcacccgat cttgaccatc tcctctgtcc cgccctcgct gatgaacacg ctcgcgcaga
     3721 tcgcggcggc tggcgcccaa acccagtcgc aggaacagct gagcatcatc ggcgcccgga
     3781 tgacggcgca gctcaaccag aagatcgccg agctcaaggc gcagggcaac gacccgatga
     3841 tcccgatcct tcagcagcaa tcggcgactt tgagccagca gcagaccagc tatgccacgg
     3901 cgcagagcca gctcgtcgcc aatggtgggc cgatcgacga tctcgacacc cagctcagca
     3961 ccatggcgac cgcggccacc gccggcgact ccgccacctt cgaccaggcg ctgagcacgg
     4021 ccaacaccga tctagccgat ctgaattccg ggccgctgct gccgggcttc cagcccgacg
     4081 gctccgcaaa cctcaaaacc aatggcctcg gcattcagtc ctcggccggt tacgacctct
     4141 cgacgccggc cggccaggcc caggcggccg ccgatatcag cgccgcgcaa acggcggtgt
     4201 cccagctctc gacggtaacg gcgcagaaca cccagatcgc catcagcgtc gaccaggcgc
     4261 tggccaccca gatcggcgcg ctcgacgacc aggtcgcttc gcgggagacg gccacgacgg
     4321 ggaacgcggc aacgcagatt caaacgctgc agacccagac ccagacccag ttccacctga
     4381 tcgagctgtc catgggcagc gcaagcaaca ccgggtccat gctgcaggat ttcgagaccg
     4441 cccaggccgc cgccgccggc cccggctcga tcctcaacct cctcgacacc caggtcccca
     4501 gcacgtcttc ctcgacgtcg aactcatcgt ccaaggcgaa ctcgtcctcc gggacgtcga
     4561 gcaacttctc gacgctcgcc taagtttctc gacgacgacg caacatcgcc ccttgtggga
     4621 gggaaagccc gccccgcgct gtggcgctcg gtcgccgaag ctccactccc cgatcgcgcc
     4681 aaagcagatg ccgacaagct ccgttgtcgg cagacgaatt tacccgctcg atggtgacaa
     4741 tacgccggat ggtcgcggcg gggacacgcg tcggcggatt acgtcccgct caggaacttg
     4801 ggatggagcg agagtaagaa cggctcagat ggtcggcgcg agccacgctc ccggccgccc
     4861 tattgcgtcg cgagccacca gcacgccgcc gtcacggcga ggagcgtgag aagcaaggcg
     4921 atgcccaagg atcggcctcg gggcaagccg atgcgccagc ggccgccgcg cacgatgagc
     4981 ttcgtcgcgg ggtcgagagc gcgctgcggc aaaagtgcgg ccaaccgctc cgccagcttc
     5041 aatacgtccg acccgctgcg cgtcgcctcg ggaaacatcg cgattgtcgg caccagcgag
     5101 cgggccgcga catcccgcgg cagatcggac agacgggccc cttcggccca gggatcggcg
     5161 ccgagccgcg tcagcgccga cagcacgctg agcggcgccc cgctcgcctc ttcgccgatc
     5221 tgagcgaaga gaaaatcgtt gagatcggag tgaagaagcg agaattgaga gggaagcgcc
     5281 atggtgacct cgatgctgcc cgggcgcgcc aatgccggtc ggttgtgccg agaaaatcag
     5341 gcggcgtcgc aattgtgcct gcgacgccgc ccggcgctca cgctgagcta caagcccggc
     5401 ggagcaaccc ttcccgccac gccgggctag aaatctccgc ccgccgccgg cggcgcgttc
     5461 ttctccgatt tctcggcgac catcgcttcg gtggtgatga gcagcccggc gatcgaggcg
     5521 gcgccttgca gcgccaggcg cacgaccttg gtcggatcga tgatgccggc ctttatcatg
     5581 tcgacatagg tgccggtctg cgcgtcgaaa ccgtaagtga cgtcgcccgt ctccaggagc
     5641 ttgccgagga tgatcgagcc atccgctccg gcattcgcca ggatctgccg cgccggcgcc
     5701 tggagcgccc ggcgcacgac gtcgatgccg acccgctgat cgtcgtttcc gggcttcaac
     5761 cgagcgagcg cctgtccggc gtagaggagt gccaggccgc cgccagcgac gatgccctcc
     5821 tcggccgcgg cgcgggtcgc atgcatggcg tcgtcgacgc ggtccttgcg ctccttcacc
     5881 tcgatatcgg tctcgccgcc gacgcggatg atggcgacgc cgccggccag cttcgccagc
     5941 cgctcctgca gcttctcctt gtcgtagtcc gacgtcgcct cctcgacctg ggcgcgcagc
     6001 tgggcgacac gggcggcgat gtccttcttc gcgccgccgc cgtcgatgat cgtcgtgtcg
     6061 tccttgtcga tctgcacttt cttggccctg ccgagcatct cgagcgtgac gttctcgagc
     6121 ttgatgccga gatcgtcgct gacgacctgg ccgccggtca ggacggcgat gtcgtcgagc
     6181 atcgctttcc gccgctcgcc gaagcccggc gccttcaccg ccgcgacctt gaggctgccg
     6241 cgcagcttgt tgaccacgag cgtcgccagc gcctcgcctt cgaattcctc ggccaccatg
     6301 agcagcggct tgccggcctt cgccacggct tcgagcaagg gcagcatgac ctggaggccg
     6361 gagatcttct tgtcgaagag caggatatag gggttctcga gctcgcagag catcttctcg
     6421 gcgttggtga cgaaatagcc cgagagatag ccgcggtcga actgcatgcc ctcgacgatt
     6481 tcgagctcgg tctcgcggct cttcgcctct tcgatcgtga tgacgccctc cttgccgacc
     6541 ttgtccatcg ccctggcgat catctcgccg atctcgcgat cgccattggc cgagatcgtc
     6601 ccgacttgca cgatctgggc gttggtcgag accgctttcg agtgcttctt gatctccgcg
     6661 acggccgcgg caacggccat gtcgatgccg cgcttcagat ccatcgggtt catgccggcg
     6721 gcgacggcct tcgcgccctc gcgcacgatc gtctgggcga gcacggtggc ggtggtcgtg
     6781 ccgtcgccgg cgagatcgct cgtctgcgcg gcgacctcct tcaccatctg cgcgcccatg
     6841 ttctcgaacc ggtcgggaag ctcgatcgcc ttggcgacgg tcaccccgtc cttggtgatg
     6901 cgcgggccgc catagggctt gtcgagcacg acgttgcggc ccttggggcc gagcgtcacc
     6961 ttcaccgcgt tggcgagaat atcgacgccg cgcaacatgc gatcgcgcgc gtcgccgctg
     7021 aatttgacgt ctttggcact catgtttcgt ctccgattgt cacgcggctt gcttcctctc
     7081 gcccggtcga tcgctcaggc taatgatcgt ccgcggactt catcacgatg aagtccttgg
     7141 catcgacgct cgaatctgca ggaatcagcg cggtcgggtc gagagagtgc cggcgcgccc
     7201 tcatgcagat ggtgctgcac acgcccggat acaacgaaca aagcaaatat cgcgaactga
     7261 gtagattccg aggctgcctc agcgctccgg cagcggatta tggtagcggt cggccagtcc
     7321 cgcatcgcga cgggcttcgt cttcccattc cgattttagc gccatggggt cgtgttgccg
     7381 ccgcgggagt gaccgccgcg gcccgcgcag gaagaccctc acccagagga gaacgcgcac
     7441 atggaacatc gcaacatctc gacagcggcc atcgctctgc tgagcggcgg tctcgtgctc
     7501 gccggctgct ccaactggtc gtcctcgcca cacgtcgacg gcagc
//
LOCUS       DASYNS010000209         7649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3790735, whole genome shotgun sequence.
ACCESSION   DASYNS010000209 DASYNS010000000
VERSION     DASYNS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3790735"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VGP50_06455"
     CDS             complement(<1..440)
                     /locus_tag="VGP50_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEV8027052.1"
                     /translation="MPWTREQMAARAAQELRDGFYVNLGIGIPTLVSNYIPSGMTVQL
                     QSENGMLGMGPFPYEGEEDPDLINAGKQTITELPTTSYFSSADSFAMIRGGHIDLSIL
                     GAMQVAENGDLANWMIPGKMVKGMGGAMDLVAGVKKVVVVMEHTA"
     gene            complement(457..1161)
                     /locus_tag="VGP50_06460"
     CDS             complement(457..1161)
                     /locus_tag="VGP50_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEV8027053.1"
                     /translation="MASKVYPSADAALAGVLRDGMVIMSGGFGLCGIADALITAIRES
                     GVKGLTVISNNAGVDGAGLGVLLESKQIKKMISSYVGENKLFAQQYLAGALELEFNPQ
                     GTLAERIRAGGAGIPAFFTKTGIGTVIAEGKEVRDFDGEKYVMERGLFADLAIIHAWK
                     ADTEGNLVYRKTARNFNPMMATAAKLTVAEVEHLVPPGDIDPDHIITPGVFVQRIVHV
                     KHPVKRIEQRTTRKRP"
     gene            complement(1212..1580)
                     /locus_tag="VGP50_06465"
     CDS             complement(1212..1580)
                     /locus_tag="VGP50_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEV8027054.1"
                     /translation="MTDTQLVLYEPKQLAVIERVLRRRLWSRLWARAASLPFAQEVLA
                     AYFCASDRRTPLAVKLTLLGALSGFLLPQRLVPKLLRSLVLGGDVAMLLGALQSFAAH
                     IQPEHRRRARLVLIRWRQER"
     gene            1667..2428
                     /locus_tag="VGP50_06470"
     CDS             1667..2428
                     /locus_tag="VGP50_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8027055.1"
                     /translation="MLVKGQAAIVTGGGSGLGAATARALARDGAKVAVFDINEANAKA
                     VAAEIGGIAVKCDVSDASSGEAAVAATRQAHGAARILVNCAGIGTAGRIVGRNGPLPL
                     DQFSRVINVNLVGSFNLMRLAAADMQKLDPLGDGERGVIISTASVAAFEGQIGQAAYS
                     ASKGGIVALTLPAAREFAGMGIRVNAIAPGLFATPMMASLPPDVQQSLGASVPFPQRL
                     GRPEEYARLALDIIANPMINGETIRLDGALRMQPR"
     gene            2483..3319
                     /locus_tag="VGP50_06475"
     CDS             2483..3319
                     /locus_tag="VGP50_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8027056.1"
                     /translation="MRELAGKAAFVTGGASGIGFALGRAFAQAGMKVMLADIETDALA
                     DAVKSLQDAGLDIRGVVCDVADPISVERAAKATCAAFGNVHVVCNNAGVGAGGGINVI
                     SLETWRWVLDVNLMGVLHGICAFLPHIRAHGEGGHIVNTASMAGMLSPGSFNPYAASK
                     FAVIAMSEGLAAQLGPLGIGVSVLCPGFVRTRISESERSRPERYGPTQIPNPASPAGA
                     LAARVAGLVQSGLDPSDVAQRVLTAIRENELYVFTHPELNEEAKQRFAVILAAMDKAA
                     SR"
     gene            complement(3345..3644)
                     /locus_tag="VGP50_06480"
     CDS             complement(3345..3644)
                     /locus_tag="VGP50_06480"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEV8027057.1"
                     /translation="MGAARIAAIKERHGWVEAEDRNAIRKIYHFGTFMEAWGFMSQVA
                     LIVGKMGDHPEWFNVYNRVEIILTTHDAGGLSQQDIRFAHLVDEIAPVRDRRAVP"
     gene            3719..4117
                     /locus_tag="VGP50_06485"
     CDS             3719..4117
                     /locus_tag="VGP50_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEV8027058.1"
                     /translation="MTAPPSLPPSIADLETLAERALGTIPAELKRHLGRVVIRVDEFP
                     DTETEVEMELDSPFDILGLYRGVSLPRKSVSDARADPDMIFLYRRPILDYWCETGEDL
                     YTLVRHVLIHEIGHHFGFSDADMERIETQD"
     gene            4130..4495
                     /locus_tag="VGP50_06490"
     CDS             4130..4495
                     /locus_tag="VGP50_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEV8027059.1"
                     /translation="MSDDDEMDEVMAGLRAQFRARLKVDGDVMRTAWRALAAVTSVDA
                     RRAALKQLLGAAHRLSGSAASFGFAPIGDAATPVEEALRAVLDPGGALALPATLAPLV
                     GELLALCDAAKPEEDSPPR"
     gene            complement(4586..6019)
                     /locus_tag="VGP50_06495"
     CDS             complement(4586..6019)
                     /locus_tag="VGP50_06495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV8027060.1"
                     /translation="MKAEIAQILSVLHPVAEITAWLVICQGMLQSAIYLGQLLLAYQT
                     LRDSPPIRRSDALWWDYGDVAMPIALLMPAYNEEATIGESIRSVLALQYPSFEVIVIN
                     DGSKDGTLQALTAAFDLKPVVRPHEQAVSHRPIRGVYGSPRYANLLVIDKENGGKSDA
                     LNAGINLSRSPLFCAMDADSLLEPDALLRAVQPFVDDPYRVVAVGGTIRVVNGCEVRS
                     GRVIRTGLPRRLLPLFQTIEYLRAFLMARLAWSRLDALTIVSGAFGIFRRSIAIEVGG
                     YTHGTVGEDLEIVIKIHRRMQEAKRDYHIRFVPEPVCWTEVPESLRVLGRQRKRWQRG
                     ALETLARHRDMILNGNYGRVGMLGLGNALLTDAFGPLFEVLGYLLVPLFWATGLLSVD
                     FLMAFLALTFVFGICISVGSLILEELELRRFPRARDLFILTLAAILENFGYRQLNNIW
                     RVLGFYQYLRGKQGWGTMTRRGFAKKT"
     gene            complement(6016..7059)
                     /locus_tag="VGP50_06500"
     CDS             complement(6016..7059)
                     /locus_tag="VGP50_06500"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV8027061.1"
                     /translation="MLLRAIWLTSVALAALSVAGMLLLIVRRMILARSERKRGQLRDA
                     LMVRVVDYLDGTGDYYHLVRLAGQHPDIAGELIGEMRQLVRGAHREKLSELVVSLGIV
                     AFNLKRLRSRDARQRRLAAAWLAVAPGEGVIAALEAALDDPDYHVRLEAVRSLTELRA
                     VRSVIEIVEKLGGEAQYNSRVLYHIFRQLVPELSGQLVELLHSGHAVPIKVLALDALG
                     KSGDGALVGPISALADDPSEELRAKALRSLGELGYPDASPFVLRALDDESWRVRTQAA
                     NCAGRIGCLAAIPRLTELLGDEQWWVSYRAAEALYVLGEEGRRALADAAARPGHAGRV
                     SELIIAEKTQMAA"
     gene            complement(7063..>7649)
                     /locus_tag="VGP50_06505"
     CDS             complement(7063..>7649)
                     /locus_tag="VGP50_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HEV8027062.1"
                     /translation="GRVDVSQRFGTFDTYYETGVDHRFNPAFAGYLYAGATPHASFRE
                     RWDVLSGGSLRVAKGGDIVGGTVLLLDLKDALYRTGFVYSANPGVQQYFLNGRLWFTA
                     KDINTWAAPGTQYLSGYLVRLDAQVTDSLLVYAGWADAPETDLNVTAEVRSIFTGLAW
                     AQSRRLTWHLDYVHDDRHDSYIRHAVIFGPSVKF"
BASE COUNT         1328 a         2504 c         2597 g         1220 t
ORIGIN      
        1 gcggtgtgct ccatcaccac cacgaccttc ttgacgccgg cgacgaggtc catggcgccg
       61 cccatgccct tcaccatctt gcccgggatc atccagttgg cgagatcgcc attctccgcc
      121 acctgcatcg cgcccaggat cgagaggtcg atatggccgc cgcggatcat ggcgaagctg
      181 tcggcgctcg agaaatagct ggtggtgggc agctcggtga tggtctgctt gccggcattg
      241 atgaggtcgg gatcctcctc gccctcatag ggaaaagggc ccatgccgag catgccgttc
      301 tcgctctgca gctgcacggt cattcccgac gggatgtagt tcgagaccag cgtcggaatg
      361 ccgatgccga gattgacgta gaaaccgtcg cgcagttcct gcgcggcgcg ggccgccatc
      421 tgttcgcgtg tccaaggcat gacggatctc ctctcctcaa ggccgcttgc gcgtggtgcg
      481 ctgctcgatg cgcttcaccg gatgcttcac atgcacgatg cgctgcacga agacgcccgg
      541 cgtgatgatg tgatcggggt cgatatcgcc gggcggcacc agatgctcga cctcggcgac
      601 ggtgagcttg gcggcggtcg ccatcatcgg gttgaagttg cgcgcggtct tgcgatagac
      661 gaggtttcct tcggtatcgg ccttccacgc atggatgatg gcgagatcgg cgaagaggcc
      721 gcgctccatc acgtatttct cgccgtcgaa gtcgcgcact tccttgcctt cggcaatcac
      781 cgtgccgatc ccggtcttgg tgaagaaggc cgggatgccg gcaccgccgg cgcggatgcg
      841 ctcggcgagc gtgccttgcg gattgaattc gagctcgagc gcgccggcga gatattgctg
      901 ggcgaagagc ttgttctcgc cgacatagga cgagatcatc ttcttgatct gcttgctctc
      961 gagaaggacg ccgaggcccg cgccgtcgac cccggcattg ttggagatca cggtgaggcc
     1021 cttcacgccc gattcgcgga tcgccgtgat caacgcatcg gcaatgccgc agaggccgaa
     1081 gccgccggac atgatgacca tgccgtcgcg caacaccccg gccaaggcgg catcggcgct
     1141 gggatagact ttgcttgcca tcgcccctca ctcccgcgga gaacgaaaat tccgctcgcg
     1201 cagcgcgcgc attatcgctc ctggcgccag cggatcagca ccagtcgggc gcgccggcga
     1261 tgctccggct ggatatgcgc agcaaagctc tgcagcgcgc ccagcagcat cgcgacgtcg
     1321 ccgcccagca ccaggctgcg caggagcttc ggcacgaggc gctgcggcag caggaaaccc
     1381 gaaagcgcgc cgagcagcgt gagcttgacg gcgagcggcg tgcggcggtc gctggcgcag
     1441 aaataggcag cgagcacttc ttgcgcaaaa ggcagcgacg ccgccctggc ccagagtcgc
     1501 gaccagagcc gccggcgcag cacgcgctcg atcacggcga gttgcttcgg ctcgtagagg
     1561 acgagttggg tgtcggtcat gaaggtataa tggcgcgatc ccgccggggt cgcaacagca
     1621 cggacgatct gctatggagc ggcaagaaaa tcgcaaggag gatcgcatgt tggtgaaggg
     1681 tcaagcggcg atcgtcacgg gcggcggctc gggtctcggc gcggccacgg cgcgagcctt
     1741 ggccagagac ggcgccaagg tcgccgtgtt cgacatcaac gaagcgaatg ccaaggcggt
     1801 ggcggcggag atcggcggca tcgccgtcaa atgcgacgtg tcggacgcgt cgagcggcga
     1861 agccgcggtg gccgcgacgc gccaggcgca tggcgcggca cgcatcctcg tcaattgcgc
     1921 gggcatcggc acggccggcc gcatcgtcgg ccgcaacggg ccgcttccgc tcgaccagtt
     1981 cagccgcgtc atcaacgtca acctggtggg gagcttcaac ctcatgcggc ttgccgccgc
     2041 cgacatgcag aagctcgacc cgctcggcga cggcgagcgc ggcgtgatca tctcgacggc
     2101 atcggtggcc gctttcgaag gccagatcgg ccaggccgcc tattcggcgt cgaagggcgg
     2161 catcgtcgcg ctcacgctgc cggcagcgcg cgaattcgcc ggcatgggca tccgcgtcaa
     2221 cgccatcgca ccgggtcttt tcgcgacgcc catgatggcg agcctgccgc ccgacgtgca
     2281 gcagagcctc ggcgccagcg tccccttccc gcagcgcctc ggccggcccg aggaatacgc
     2341 ccgcctcgcc ctcgacatca ttgccaaccc aatgatcaat ggcgagacta tccggctcga
     2401 cggcgcgctg cgcatgcaac cgaggtaatg cggcccggcc tcgccccgtc gagcgcgtcg
     2461 cgttgcggga ataggaggaa tgatgcgtga gcttgctggc aaggcggcct tcgtgaccgg
     2521 cggagcgagc ggcatcggct tcgcgctcgg ccgggccttt gcgcaagcgg gcatgaaggt
     2581 gatgctcgcg gatatcgaga cggacgcgct ggcggacgcc gtcaagagcc tgcaggatgc
     2641 cgggctcgat atacgcgggg tcgtctgtga cgtcgccgat ccgatcagcg tcgagcgcgc
     2701 ggccaaggcg acctgcgcgg cattcggaaa tgttcatgtc gtctgcaaca atgccggcgt
     2761 cggcgccggc ggtggcatca acgtcatctc gctcgagacg tggcgatggg tgctcgatgt
     2821 caacctcatg ggcgtccttc acggcatctg tgccttcctt ccgcacatcc gcgcccatgg
     2881 cgaaggcggc catatcgtca acaccgcctc gatggccggc atgctgagcc ccggctcgtt
     2941 caacccttac gcagccagca agttcgcggt gatcgcgatg tcggaggggc tggcggcgca
     3001 gctcgggcca ctcggcatcg gcgtgagcgt gctctgcccc ggatttgtcc gtacccgcat
     3061 cagcgagagc gagcgcagcc ggccggagcg ctacggtccg acgcagatac cgaaccccgc
     3121 cagcccggca ggcgcgctcg ccgcccgggt cgccggactc gtacagtccg ggctcgaccc
     3181 gtcggacgtc gctcagcgag tcctgaccgc gatccgcgag aacgagctct atgtgttcac
     3241 ccatccggaa ctgaacgagg aggctaagca gcggtttgcc gtcatcctgg ccgcgatgga
     3301 caaggcggct tcccgctaga gccggtgcga tacgcggccg gccatcacgg gaccgcgcgg
     3361 cggtcgcgca ccggcgcgat ctcgtccacg agatgggcga agcggatgtc ctgctgggag
     3421 agaccgcccg cgtcatgggt ggtgaggatg atctcgacgc gattgtagac gttgaaccat
     3481 tcggggtgat cgcccatctt cccgacgatg agcgccacct ggctcatgaa gccccacgcc
     3541 tccatgaagg tgccgaagtg atagatcttg cggatggcat tgcggtcctc ggcctcgacc
     3601 cagccatggc gttccttgat cgcggcgatg cgcgccgcgc ccaccaatcg ctcgaccgtc
     3661 gcccttctcc agtggcgggt agcgggcgcg agatcaagcg ccggccggaa ggaaaaccgt
     3721 gaccgcaccg ccaagtctgc cgccgtcgat tgccgacctc gaaacgcttg ccgagcgcgc
     3781 gctgggcact atcccggcgg aactgaagcg gcatctgggc cgcgtcgtga tccgcgtcga
     3841 cgagttcccc gacaccgaga ccgaggtgga gatggagctc gacagcccct tcgacatcct
     3901 cggcctctat cgcggcgtgt ccctgccacg caagagcgtg agcgacgcgc gggccgatcc
     3961 cgacatgatc tttctctacc gccgtcccat cctcgactac tggtgcgaga ccggcgagga
     4021 tctctacacg ctggtccgcc atgtcctcat ccacgagatc ggccatcact tcggcttcag
     4081 cgacgccgat atggagcgga tcgagacgca ggattaaccc gggtcaacca tgtccgacga
     4141 cgacgagatg gacgaggtga tggccgggct gcgcgcccag ttccgcgcgc ggctcaaggt
     4201 cgatggcgac gtcatgcgca ccgcctggcg cgcgctcgcc gcggtgacga gcgtcgatgc
     4261 gcgccgtgcc gccttgaaac agttgctggg cgccgcgcat cggctctcgg gcagcgccgc
     4321 cagcttcggc ttcgcgccga tcggcgacgc cgccacgccg gtggaggagg cgctccgggc
     4381 cgtgctcgac cccggcggtg cacttgcgct gccggcgacg ctcgcgccgt tggtgggtga
     4441 gcttctcgcg ctttgcgatg cggcgaaacc agaagaagat tcaccaccga gatagtgaga
     4501 aacgaagaat gcgcgcaagg cgcgcatcca atgtttttcc tctcactgcc tcattgtctc
     4561 ggtggtgaac ctttcttgct tttcctcacg tcttcttggc gaagccgcgc cgcgtcatcg
     4621 ttccccagcc ctgcttgccg cgcagatatt ggtagaaacc caggacgcgc cagatgttgt
     4681 tgagctggcg gtagccgaaa ttctcgagga tcgcggccag cgtcaggata aagaggtcgc
     4741 gggcgcgagg aaagcgccgg agctccagct cttcgagaat gagcgagccg acgctgatgc
     4801 agatgccgaa gacgaaggtg agcgcgagga aagccatgag gaagtcgacg ctcagcaggc
     4861 ccgtcgccca gaacagcggg accagcagat agcccagcac ctcgaaaagc gggccgaacg
     4921 catcggtgag gagcgcgttg ccgaggccta gcatgccgac gcggccatag ttgccgttga
     4981 ggatcatgtc gcggtgtcgc gccagcgtct cgagcgcgcc acgctgccag cgcttcctct
     5041 gtcggccgag gacgcgaagg ctctcgggca cctcggtcca gcagactggc tcgggcacga
     5101 agcggatgtg atagtcgcgc ttcgcctcct gcatgcggcg atgaatcttg atgacgatct
     5161 cgaggtcctc gccgacggtg ccgtgcgtgt agccgccgac ctcgatggcg atggagcggc
     5221 ggaagatgcc gaaggcgccg gagacgatgg tcaacgcgtc gagccggctc caggccaggc
     5281 gcgccatcag gaaagcgcga agatattcga tcgtttgaaa gagcggcagg aggcggcgcg
     5341 gcaatccggt gcggatgacg cggccgctac ggacctcgca gccattgacg acgcggatcg
     5401 tgccgccgac ggcgacgacg cggtagggat cgtcgacgaa aggctgcaca gcgcgcagca
     5461 acgcgtcggg ctcgagcagc gaatcggcgt ccatggcgca gaagagcggc gagcgcgaga
     5521 gattgatgcc ggcgttgagg gcatcggact tgccgccatt ctccttgtcg atcaccagga
     5581 gattggcata gcgcggagag ccgtagacgc cgcggatcgg gcgatgcgac accgcctgct
     5641 catggggccg caccacaggc ttcaggtcga aggcggcggt cagcgcctgg agcgtgccgt
     5701 ccttcgagcc gtcattgatg acgatcacct cgaagctcgg atattgcagc gccagcaccg
     5761 agcggatgct ttcgccgatc gtcgcttctt cgttgtaggc gggcatcagc agcgcgatcg
     5821 gcatggcgac gtcgccataa tcccaccaca gcgcgtcgct gcggcggatg ggcgggctgt
     5881 cacgcagcgt ctgataggcg agtagaagct ggcccagata gatggcgctt tgcagcatgc
     5941 cctggcagat gacgagccag gcggtgatct ccgccaccgg gtgcagcacc gagagaattt
     6001 gcgcgatctc ggccttcatg ccgccatctg cgtcttctcg gctatgatca gctcggagac
     6061 gcgtccggcg tggccggggc gcgcggcggc gtcggccagc gcgcggcgcc cctcctcgcc
     6121 cagcacataa agcgcctcgg cggcgcgata gctcacccac cattgctcgt cgccgaggag
     6181 ctccgtgagg cgcggtatgg cggcgaggca accgatgcgc ccggcgcaat tcgccgcctg
     6241 cgtccgcacg cgccaggact cgtcgtcgag cgcgcgcagc acgaaaggcg aggcgtccgg
     6301 atatcccagc tcgccgagag agcgcagcgc cttggcacga agctcctctg acggatcgtc
     6361 ggcgagcgcc gagatcggcc ccaccagcgc gccatcgccg ctcttgccga gcgcatcgag
     6421 cgccaacacc ttgatcggca ccgcgtgccc ggagtgcagc agctccacca gctggccgct
     6481 gagctcgggc acgagctggc ggaagatgtg gtagagcacg cgcgaattgt actgcgcctc
     6541 gccgccgagc ttctcgacga tttcgatcac cgagcggacg gcgcgcagtt cagtgagcga
     6601 gcgcaccgcc tcgagccgca catggtagtc gggatcatcg agcgccgcct cgagcgcggc
     6661 gatcacgccc tcgccgggcg ccacggcgag ccacgcggcg gcgaggcggc gctgccgcgc
     6721 gtcgcgactc ctgagccgct tcaagttgaa cgcgacgatg ccgagcgaga ccaccagctc
     6781 agagagcttc tcgcgatggg cgccgcgcac gagctggcgc atctcgccga tgagctctcc
     6841 ggcgatgtcc ggatgctgtc ccgccaacct gacaaggtgg taatagtcgc ccgtgccgtc
     6901 gagatagtcg acgacgcgca ccatgagcgc gtcccgcaat tgcccgcgct tgcgttcgct
     6961 gcgcgcgaga atcatgcggc ggacgatcag caacagcatg ccggcgaccg agagcgcggc
     7021 cagtgccacg gaggtcagcc agatcgcccg caggagcatg gctcagaact tcacgctcgg
     7081 gccgaagatc acggcgtggc ggatatagga gtcgtgacgg tcgtcatgga catagtcgag
     7141 atgccaggtg agccgccgcg actgcgccca ggcgaggccg gtgaagatgc tgcgcacctc
     7201 ggccgtgaca ttgagatcgg tctcgggcgc gtcggcccag ccggcgtaga cgagcagcga
     7261 atccgtgacc tgcgcgtcga ggcgcaccag gtagcccgag agatactgcg tgccgggagc
     7321 ggcccaggtg ttgatgtcct tggcggtgaa ccagagccgc ccgttcagga aatattgctg
     7381 cacgcccggg ttcgccgagt agacgaagcc ggtgcggtag agcgcatcct tgaggtcgag
     7441 cagcagcacc gtgccgccga cgatgtcgcc gcccttcgcc acgcgcaggc tgccgcccga
     7501 gagaacgtcc cagcgctcgc ggaaggacgc gtgcggcgtg gccccggcat agagatatcc
     7561 cgcgaacgcc gggttgaagc ggtgatcgac gccggtctcg tagtaggtgt cgaaggtgcc
     7621 aaagcgctgg ctgacatcga cgcgtccat
//
LOCUS       DASYNS010000210         7587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2698290, whole genome shotgun sequence.
ACCESSION   DASYNS010000210 DASYNS010000000
VERSION     DASYNS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2698290"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VGP50_06510"
     CDS             <1..637
                     /locus_tag="VGP50_06510"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEV8027063.1"
                     /translation="ELRALLAQGRQIEAIRRYRLATGADLATAKTAIDRLGRANARVS
                     VGGVVRLLAAFFWLCAILAATAAGWQAHERRIVNDTWPQVDAEVVKCSVVTHTPMRSP
                     TFATLACQFRYRVDRVDHTANTGSTGIPSVAQEAAMHAWVAQHRPGSRQVIRYDPADP
                     QRISLGDADAAFQADTPGLRLRLAGLFTGGGVLFVALAAWLAARRRRDDAS"
     gene            785..1417
                     /locus_tag="VGP50_06515"
     CDS             785..1417
                     /locus_tag="VGP50_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEV8027064.1"
                     /translation="MAPLTFYYGSGSPYAWRVWFALEIKSFAYEMKTLSFDAGEHKRP
                     EFLALNPRGRVPVLVDGGFALYESAAIVEYLEDIKPGEPRLFAAAPRSRAVQRRMMRE
                     ADQYVGPALQQLALALRAPAGDEKIAAATTELQREFASWETGVAGDYLTGPLSAADIT
                     LYPQVALAQRVLTRAKGAVPAGFLGPKLSAWARRMEALPATRKTWPPHWK"
     gene            complement(1468..3840)
                     /locus_tag="VGP50_06520"
     CDS             complement(1468..3840)
                     /locus_tag="VGP50_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEV8027065.1"
                     /translation="MAAFSSRPASSLTRPSPLAESDTLALLTALERKVLWLSSWTIHN
                     ANHLRPNRDGLKVGGHQASSASVATLLTALYFDVLRPEDRVAVKPHASPVYHAIQYLL
                     GRQSREKLERFRALGGAQSYPSRSKDGDDVDFSTGSVGLGVAVTSFAALVQDYVHLKG
                     LTPEAQPRGRMVAVVGDAELDEGNVFEALLEGWKHDVRNLWWVIDYNRQSLDTVVSDR
                     LFGRIDALFDMMGWRVVTLKYGKALEAAFAGPDGDALRQWIDACPNSLYSALVFKGGE
                     GWRKHLEGDLGQLPGIRGFLAAHDDASLARVMTNLAGHDMAAVLEAFHGIADDRPTCF
                     IAYTIKGFGLPFAGHKDNHAGLMNEEQMASFRRAMAIPDGAEWDRFAGLKLPPETIER
                     FLAGVAFAGRSVRRLTAPAVAIPEALPLPRAARMSTQEGFGRILNDIAAEDSELARRI
                     VTCSPDVTVSTNLGGWVNRRGVFDRREREDVFRDEKVISAQRWQMSPKGQHIELGIAE
                     HNLFLLLAALGLSAPLFGARLLPIGTLYDPFISRGLDALNYACYQDARFMVVATPSGI
                     TLAPEGGAHQSIGTPLIGMAQDGLAAFEPAFVDELAVLLRWGFLHLQAEEGGSVYLRL
                     STRPLEQPQRTIDPALAADIIAGGYWLKPPSPGADLAIVFTGAVAPEAIAAHEMLLED
                     IPSAGLLAVTSADRLHAGWVEGARSGEASHASRLLAPLAPDAAMVTVIDAHPATLSWL
                     GAVAQHRVFPLGVEHFGQSGDIPDLYRVYGLDSDAILAAAARACLGKIRQ"
     gene            complement(3934..4215)
                     /locus_tag="VGP50_06525"
     CDS             complement(3934..4215)
                     /locus_tag="VGP50_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027066.1"
                     /translation="MSLNFLNGKELAARFWRRPETPTDIQPGNRYCRKRRDNVTETAT
                     VLELRPDPFGIPHVRFTLAFEQPSLGCVSETRRVLALASFVENYSERVA"
     gene            complement(4365..5804)
                     /locus_tag="VGP50_06530"
     CDS             complement(4365..5804)
                     /locus_tag="VGP50_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HEV8027067.1"
                     /translation="MSERGTTSGFLDGVFGRTLGNLRSLRSAWRDIASQARDALSSAP
                     KPDLSRDDAERLRAQLRACLEARGGAVSARARAADLGRTYLALNATGRERFLRLLAEE
                     FDVDRAVIAELCGRLLNVKDGARRDIEQALRAALEPPRVKLLTQFNALPEGVKFLVDM
                     RAELLTLARHDAVLAGLERDLKALLASWFDIGFLELKRITWEAPAALLEKLILYEAVH
                     EIRSWADLKNRLEADRRCFAYFHPRMPEEPLIFVEVALVRGMADDIQALLDETAPVGD
                     PIDADTAIFYSISNCQRGLAGISLGDFLIKRVVDRQAAELPHIKTYATLSPIPGFRAW
                     LDQRVREEGEDLLTPIERKALQQLPGELQGRDIGALLKWPRWPEDTAVRDALKAPLLR
                     LCARYLLTEKNPKGQALDPVAHFHLTNGARVERLNWLADLSGKGLQQSAGLMINYLYR
                     LNEIEENHEAYTAAGRIVASGQLSRLAKG"
     gene            5993..6502
                     /locus_tag="VGP50_06535"
     CDS             5993..6502
                     /locus_tag="VGP50_06535"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEV8027068.1"
                     /translation="MTAIRGLLAAAAALAAAGFGAAAVADTPLTPPKGFRQWFHVNSE
                     VVTKDSALFDAFGGMHNVYLNPGGVPMLKNGKPYPDGTTFVDEIRAFIIQDGTYVPGD
                     RKVLAVMVRNGKKYAATGGWGFQAWAANDPKKPLVTDAVRQCFGCHTQKVAIPRPGWH
                     EYVYSTYIP"
     gene            complement(6663..>7587)
                     /locus_tag="VGP50_06540"
     CDS             complement(6663..>7587)
                     /locus_tag="VGP50_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV8027069.1"
                     /translation="FRKKPGHLLLPGRALSGEIVLADIGIPAAVLGAIAPKTHENGPA
                     LWLDAYPWPRLDTHKYQRGHALIAGGAVMTGAARLAARAAQRIGAGLVTLAAPAAAWP
                     VYAASLTGVIVRPEDFAALLGDVRRNTLLLGPGLGADEATRAWVLAALATKRAVVLDA
                     GALTAFAAAPATLFAAIQGPAVMTPHEGEFARIFRLEGDKLARARAAAAESGAVVLLK
                     GSDSVIAAPDGRAAINANAPPDLATGGSGDVLAGLVAGLLAQGLDPFRAAAAACWLHG
                     DAAQDFGPGLIAEDLVDCLPGALRRLKGVAV"
BASE COUNT         1254 a         2560 c         2625 g         1148 t
ORIGIN      
        1 cgagctcagg gcgcttctgg ctcagggccg gcagatcgag gcgatccggc gctatcgcct
       61 tgccactgga gccgatctcg ccaccgccaa gaccgctatc gaccgcctcg gccgtgccaa
      121 cgcgcgcgtc tctgtgggtg gtgtcgtcag gctcctcgcc gcctttttct ggctctgcgc
      181 gatcctcgcg gccaccgcgg ccgggtggca agcccatgag cggcgcatcg taaacgacac
      241 ctggccgcag gtcgacgctg aagtcgtgaa gtgctcggtg gtgacgcata cgccgatgcg
      301 cagcccgacc ttcgcgacgc tggcctgtca gttccgctac cgcgtggacc gcgtcgacca
      361 cacggcgaac accggcagta ccggcattcc ttcggtcgcg caggaagcgg cgatgcatgc
      421 ctgggtcgcg cagcatcgcc cgggcagccg gcaagtcatt cgctatgatc ccgccgatcc
      481 gcagcgcatc agcctcggcg atgccgacgc cgcgttccag gccgatacgc ccggactacg
      541 cctgcgcctc gccggcctct tcaccggcgg cggcgtcctc ttcgtcgccc tcgcggcatg
      601 gctcgcggcg cgcaggcggc gtgacgacgc gtcctgagga aaggacgtcg tcagggtcga
      661 acggcgcaac ccacctctgc gggctgcgcc gtggtgaccc tcccccgatc gcggtagctt
      721 cggacgatcg tctggtcccg ccacgatcgc gtggaccgac cggtgcaacg gaggcgcgac
      781 ggcgatggct cccctcacct tctactacgg ctctggctca ccctatgcgt ggcgcgtctg
      841 gttcgcgctc gagatcaaaa gcttcgccta cgagatgaag acgctgtctt tcgatgccgg
      901 cgagcacaag cggccggagt tcctggccct gaacccgcgc ggccgcgtgc cggtcctcgt
      961 cgatggcggc ttcgcgctct acgaatccgc ggcgatcgtc gagtatctcg aggacatcaa
     1021 gccgggcgag ccgcgcctct tcgccgccgc tccgcgttcc cgcgccgtgc agcggcgcat
     1081 gatgcgcgaa gccgatcaat atgtcggccc ggcgctgcag cagctcgccc tggcgctccg
     1141 tgcccctgcc ggcgacgaga agatcgccgc cgccactacc gagctgcaac gggagttcgc
     1201 gtcgtgggag acgggggtcg ccggcgacta tctcaccggc ccgctctccg ccgcggacat
     1261 cacgctctat ccgcaggtag cgctcgcgca gcgcgtcctg acccgcgcca aaggcgcagt
     1321 gcccgcgggc ttcctcggcc cgaagctgag cgcctgggcg cgccgcatgg aagcgctgcc
     1381 cgccacgcgg aagacctggc cgccacactg gaagtaactt ccctcgcacg ccgcaatccg
     1441 gcagcgcgcc tcaaacatct cactgtctca ctgacgaatc ttaccgagac acgctcgcgc
     1501 cgccgccgcg aggatcgcgt cgctgtcgag accgtagacg cgatagagat cggggatgtc
     1561 gcccgactgt ccgaaatgct cgacgcccag cggaaagacg cgatgctgcg ccaccgcgcc
     1621 gagccaggag agcgtcgccg ggtgggcgtc gatgacggtc accatcgccg catccggcgc
     1681 gagtggcgcc aggagccgcg acgcgtgact cgcttcgccg ctccgcgcac cctcgaccca
     1741 gccagcatga aggcggtccg ccgaggtgac ggcgaggagg ccggcactgg gtatgtcctc
     1801 gagcagcatc tcgtgagcgg cgatggcttc gggcgcgacg gcgcccgtaa agacgatcgc
     1861 gaggtcggcg cccggtgacg gcggcttcag ccaatagccg ccggcgatga tgtcggcggc
     1921 gagggccggg tcgatcgtgc gctgcggctg ctcgaggggg cgcgtcgaga ggcgcagata
     1981 gacggagccg ccctcctccg cttgaagatg gagaaagccc cagcgcagca gcaccgcgag
     2041 ctcgtcgacg aaagcgggct cgaaagcggc gaggccgtcc tgcgccatgc cgatgagcgg
     2101 cgtcccgatc gattgatgcg cgccgccttc gggcgcgagt gtgatacccg agggcgtcgc
     2161 caccaccatg aagcgcgcat cctggtagca ggcgtagttg agcgcatcga ggccgcggct
     2221 gatgaaggga tcgtagagcg tgccgatcgg cagcagccgc gcgccgaaga gcggcgccga
     2281 gagcccgagc gccgccagca gcagaaagag attgtgctcg gcgatgccga gctcgatgtg
     2341 ctggcccttg ggcgacatct gccagcgctg cgccgagatc accttctcgt cgcggaagac
     2401 gtcttcgcgc tcgcgccggt cgaagacgcc gcgacgattg acccagccgc cgagattggt
     2461 cgacaccgtt acatcgggcg agcaggtgac gatgcgccgc gccagctcgc tgtcctcggc
     2521 tgcaatgtcg ttgaggatcc gcccgaaacc ctcctgggtc gacatccgcg ccgcgcgcgg
     2581 cagcggcagc gcctcgggta ttgccaccgc cggcgcggtc agccggcgca cgctacgccc
     2641 ggcaaaagcc acgccggcga gaaagcgctc gattgtctcg ggcggcagct tcaggccggc
     2701 gaagcggtcc cattcggcgc cgtcgggaat cgccatggcg cgccggaagc tcgccatctg
     2761 ctcttcgttc atgaggccgg catggttgtc cttgtggccg gcgaaaggca ggccgaagcc
     2821 tttgatggtg taagcgatga agcaggtcgg gcgatcgtcg gcgatgccgt ggaaggcctc
     2881 gagcaccgct gccatgtcat ggcccgcgag attcgtcatg acgcgtgcca ggctcgcgtc
     2941 gtcatgcgcc gccaggaagc cgcggatgcc cggcagttgc ccgagatcgc cttcgagatg
     3001 cttgcgccac ccctcgccgc ccttgaagac gagcgccgag tagagcgagt tgggacaggc
     3061 atcgatccat tgacggagcg catcgccgtc cggcccggcg aaggccgctt cgagcgcctt
     3121 gccgtatttg agggtgacga cgcgccagcc catcatgtcg aagagcgcat cgatgcgtcc
     3181 gaagaggcgg tcgctcacga cggtgtcgag actctggcgg ttgtagtcga tcacccacca
     3241 gagattgcgc acgtcatgct tccagccttc gagcagcgcc tcgaagacat tgccttcatc
     3301 gagctcggca tcgccgacga cggccaccat ccgcccgcgc ggctgcgcct cgggcgtcag
     3361 gcctttcaga tggacatagt cctgcaccag cgccgcgaag ctggtcacgg caacacccaa
     3421 gcccaccgat cccgtcgaga aatcgacgtc gtcgccatcc ttgctgcgcg agggatagga
     3481 ctgagcgccg cccaaggcgc ggaagcgctc gagcttctcg cgcgactgcc gtccgaggag
     3541 atactgaatg gcgtgataga ccgggctcgc atggggcttg acggcgacgc ggtcctcggg
     3601 ccggagaaca tcgaaataga gcgcggtcag cagcgtcgcg accgaggcgc tcgaggcctg
     3661 atgtcccccc actttgagcc cgtcgcggtt gggacgcagg tgattggcat tgtggatcgt
     3721 ccacgaggag agccacagca ccttgcgctc gagcgctgtt agaagcgcca gcgtatcgct
     3781 ctcggccagc ggcgacggcc gggtgagcga agatgcgggc cgagacgaga acgcggccat
     3841 accggcctcc ccatcgagcg ggccagagcg gacgcgaaac tatacgggat tagcggagag
     3901 ttggcgaaac ctgcgggagg cggccggcag ggatcaggcg acgcgctcgc tgtagttctc
     3961 gacgaagctc gcgagggcca gcacacggcg ggtctcgctc acgcagccca gtgagggctg
     4021 ctcgaaggcg agcgtgaagc ggacatgcgg gatgccgaag ggatcggggc gaagctcgag
     4081 gacggtcgcg gtctcggtga cgttgtcgcg gcgcttgcgg cagtagcggt tgccgggctg
     4141 gatgtcggtc ggagtctcgg ggcgacgcca gaagcgcgcc gcgagttctt taccgttgag
     4201 gaagttgagc gacatctgcc gcctgtgagc tacgtgttca tgcctggaat tgacgctatc
     4261 ccgcaaaagt taagcagaag cgaaaaccgc ttaaccggcg agtgatttcg gagagaaagc
     4321 ccttcaattt tagttgaaaa gccgcggccg cgagccccgc ggattcagcc ttttgccagc
     4381 cggctcaatt ggccggaggc gacgatcctg cccgcggctg tataagcctc atggttttct
     4441 tctatctcgt tcagccggta gagatagtta atcatgagcc ccgccgattg ctgcagccct
     4501 ttgcccgaca gatcggcaag ccaattgagc cgctccacac gagcgccgtt ggtcagatgg
     4561 aaatgggcaa ccggatcaag cgcctgtcct ttcgggttct tctcggtcag gagatagcgg
     4621 gcgcagaggc gcaggagtgg cgccttgagc gcgtccctca cggcggtgtc ctcaggccag
     4681 cgcggccact tcaagagcgc gccgatgtcc cggccctgga gctcgcccgg caattgctga
     4741 agcgccttgc gctcgatcgg cgtcagaaga tcctcgcctt cctcgcggac gcgctggtcc
     4801 agccaggcgc ggaagccggg gatcggcgac agcgtcgcat aggtcttgat gtgcggcagc
     4861 tcggcagctt ggcggtcgac cacccgtttg atcagaaaat cgcccaggct gatgccggcg
     4921 aggccgcgct ggcagttcga gatcgaatag aaaatggcgg tgtcggcgtc gatcggatcg
     4981 cccaccggcg ccgtctcgtc gagcagcgcc tggatgtcgt cggccatgcc ccgcacgagc
     5041 gccacctcga cgaagatcag cggctcctcc ggcatccgcg gatggaaata agcgaagcat
     5101 cgccggtccg cctcgaggcg gttcttgaga tcggcccagc tgcggatctc gtgcaccgcc
     5161 tcataaagga tgagcttctc cagcagcgcc gccggcgcct cccaggtgat gcgcttcaac
     5221 tcgagaaagc cgatatcgaa ccagctggcc aggagcgcct tcaggtcgcg ctcgagcccg
     5281 gcgaggaccg cgtcgtgccg cgccagtgtc aggagctcgg cgcgcatgtc gaccaggaac
     5341 ttgacaccct cggggagcgc gttgaattgg gtgagcagct tgacccgggg cggctcgagc
     5401 gccgcgcgca gcgcctgctc gatgtcgcgg cgcgcgccgt ccttcacgtt gaggaggcgc
     5461 ccgcagagct cggcgatcac ggcgcggtcg acgtcgaatt cctcggcgag gagcctgagg
     5521 aaacgctcgc gtcccgtggc gttgagcgcg agataggtgc ggccgagatc ggcggcgcgc
     5581 gcgcgggcgg agacggcgcc gccccgcgcc tcgaggcagg cgcgcagctg cgcgcgcagg
     5641 cgctcggcgt cgtcgcgcga gaggtccggc ttcggcgccg agctgagggc gtcccgcgcc
     5701 tgcgacgcga tatcgcgcca ggcgctgcgc agcgagcgca gattgccgag ggtgcggccg
     5761 aagacgccgt cgaggaagcc gctggtggtg ccgcgctcgc tcatgcgctt cgggataaac
     5821 tgcctgcgag gcgatggcaa gggcgtgcac cacgaccctg ggatgcttgc tggtccggcg
     5881 aaaaacatgc attttgagag agatgcgaga gatctgcgat ccgccggaac aaggcgggcg
     5941 caagctgggg agccgcccgc tccatcgcat gcccgccgaa gggggacgaa tcttgacagc
     6001 gatacgaggt cttctcgccg ccgcggccgc actcgctgcg gccggcttcg gcgctgccgc
     6061 cgtcgccgac acgccgctga caccgcccaa gggtttccgt cagtggtttc acgtcaacag
     6121 cgaggtggtg accaaggaca gcgcgctgtt tgacgctttc ggcggcatgc ataacgtcta
     6181 tctcaacccg ggcggcgtgc ccatgctgaa gaacggcaaa ccctatcccg acggcaccac
     6241 tttcgtcgac gagatccggg ccttcatcat ccaggacggc acctatgtcc ccggcgaccg
     6301 caaagtgctg gcggtcatgg tgcgcaacgg caagaaatac gccgccaccg ggggctgggg
     6361 cttccaggcc tgggccgcaa acgatcccaa gaagccgctg gtcaccgacg ctgtccggca
     6421 atgtttcggc tgccacaccc agaaggtcgc catcccccgc cccggctggc acgaatacgt
     6481 ttactcgacc tacattccct gagcgaacgc gcgaacgcgc gttcgtcgaa agcgggcgcc
     6541 agccgttgct cttgcaaccg cgagagcccg caccgtccgg cgcggcgcga agcgtcgtcg
     6601 ccggcgggcg ccagcggttg ctcttgcaac cgcgagagcc cgcaccgtcc agcgtggcga
     6661 tctcagaccg ccaccccctt cagccgccgc agcgcccctg gcaggcaatc gaccagatcc
     6721 tcggcgatga ggccgggccc gaagtcctgg gcggcgtcgc catggagcca gcaggcggca
     6781 gcggcggcgc ggaacggatc cagaccttgc gcgagaagcc cggccacgag gccggcgagg
     6841 acgtcgccgc tgccgccggt cgcgagatcg ggcggcgcgt tggcattgat ggctgcgcgc
     6901 ccatccggcg cggcgatgac gctgtcgctg cctttgagga gcaccacggc gccactctcg
     6961 gccgccgcgg cgcgggcgcg ggcgagcttg tcgccctcga gcctaaagat ccgggcgaac
     7021 tcgccttcat ggggggtcat gactgccggg ccctggatgg cggcgaagag cgtcgccggc
     7081 gcggcggcga aggccgtgag ggcgccggca tcgaggacga cggcgcgctt cgtcgcaagc
     7141 gccgccagca cccatgcccg cgtcgcctca tccgcgccga ggccgggtcc cagcagcaac
     7201 gtgttgcgcc ggacatcgcc gaggagtgct gcgaaatcct ccggccgcac gatgacgccg
     7261 gtgagtgaag ccgcatagac cggccaggct gccgcgggcg ccgcgagggt gacgagcccg
     7321 gcgccgatgc gctgggccgc gcgcgcggcg agccgtgccg cgccggtcat gacggcgccg
     7381 ccggcgatca gcgcatggcc gcgctgatat ttgtgcgtgt ccagccgcgg ccagggatag
     7441 gcgtcgagcc agagcgccgg gccgttttcg tgggtcttgg gcgcgatggc cccgagcacg
     7501 gcggcgggaa tgccgatatc ggcgagcacg atctcgcctg aaagagcgcg gcccgggagc
     7561 agcagatggc cgggcttctt gcgaaag
//
LOCUS       DASYNS010000211         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3946728, whole genome shotgun sequence.
ACCESSION   DASYNS010000211 DASYNS010000000
VERSION     DASYNS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3946728"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VGP50_06545"
     CDS             complement(<1..705)
                     /locus_tag="VGP50_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV8027070.1"
                     /translation="MSYDQGSRWASRGAATQVGVDAGLRQYMLRVYNYMAAGLALTGV
                     VAYGAVASGFYQTISGTPLVWLVILAPFAFVLVLSFGIQRLSLSAAQLTFWAFAAVMG
                     LSLASIFLIYTHTSIARVFFITAGTFAGMSLYGYTTQRDLTGIGSFLIMGLWGIVIAG
                     VVNLFLASSALYFATSVIGVFIFVGLTAYDTQRIKETYFESDGNVLAGKKAIMGALSL
                     YLDFINLFLLLLRFFGD"
     gene            complement(804..>1536)
                     /locus_tag="VGP50_06550"
     CDS             complement(804..>1536)
                     /locus_tag="VGP50_06550"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV8027071.1"
                     /translation="PKDYDGPPLVSMDAEIARGMGLKLGQTITVNVLGRDVTATIANL
                     REIDWTALGINFVMVFSPGMLEGAPQTHIATARVPPEEEAALERAVTDRFPNVSAIAV
                     KDALAAVAQIIAAIATALRATAAITLIAGTLVLAGAVAAGHHRRVYEAVVLKVLGATR
                     SDITRAFLIEYGILGLATAVLAAGLGTLSAYLILTRVMHQDWTFLPAAVAATALIAGV
                     LTLGIGYAGTWKALGAKAAPFLRNE"
BASE COUNT          292 a          464 c          557 g          223 t
ORIGIN      
        1 gtcgccgaag aagcgcagca ggagcaggaa gaggttgatg aagtcgagat agagggagag
       61 cgcgcccatg atcgccttct tgccggcgag cacgttgcca tcgctttcga aatacgtttc
      121 cttgatgcgc tgcgtgtcgt aggcggtgag cccgacgaag atgaacacgc cgatcaccga
      181 ggtggcgaaa tagagggcgg acgacgcgag gaagaggttg acgacaccgg cgatgacgat
      241 cccccagagt cccatgatca ggaacgagcc gatgccggtg agatcgcgct gcgtcgtgta
      301 gccgtagagg ctcatgccgg cgaaggtgcc ggcggtgatg aagaagacgc gggcgatgct
      361 ggtgtgcgta taaatcagga agatcgacgc gagcgacaga cccatgaccg cggcgaaggc
      421 ccagaaggtg agctgcgccg cgctgaggct caggcgctgg atgccgaagc tcaacacgag
      481 cacgaaggcg aagggcgcga ggatgaccag ccagacgagc ggcgtgccac tgatggtctg
      541 gtagaagccg ctggcaacag ccccgtaagc gaccacgccg gtgagcgcca ggccggccgc
      601 catgtagttg tagacgcgca gcatgtactg gcgcaggccg gcatcgaccc cgacctgcgt
      661 ggcagcgccg cgcgacgccc aacggctacc ctgatcgtat gacatgacca acctctaagg
      721 ttgaaggtat cgcccgcaca tattggcatg cggacgggta aattcaatgt tagcgggcac
      781 ccgcctcaat ggcggtcccg ccctcactcg ttgcgcagga acggcgccgc cttggcgccg
      841 agcgccttcc aggtcccagc atagccgatg ccgagggtca atacaccggc gatgagcgcc
      901 gtcgccgcca ccgccgccgg gaggaaggtc cagtcctggt gcatgacccg ggtcaggatc
      961 agataggccg agagcgtgcc gagaccggcc gcgaggacgg ccgtggcgag gccgaggatg
     1021 ccgtattcga tgaggaaggc gcgcgtgatg tcgctgcgcg tcgcgcccag gaccttgagc
     1081 accaccgcct cgtagacgcg gcggtgatgg ccggccgcga cggcgccggc gagcacgagc
     1141 gtcccggcga tgagagtgat ggcggcggtg gcgcgcagcg ccgtggcgat cgcggcgatg
     1201 atttgcgcca cggcggcgag cgcgtccttg acggcgatgg ccgagacatt ggggaagcgg
     1261 tcggtgaccg cgcgctcgag cgccgcctct tcctcgggcg gcacgcgcgc ggtcgcgata
     1321 tgcgtctgcg gcgctccttc cagcatgccc ggcgagaaga ccatgacgaa gttgatgccg
     1381 agcgcggtcc agtcgatctc gcgcaggttg gcgatggtgg cggtgacgtc gcgccccagt
     1441 acgttgacgg tgatggtctg gcccagcttc aggcccatgc cgcgcgcgat ctcggcatcc
     1501 atcgagacga ggggcgggcc atcgtaatcc ttgggc
//
LOCUS       DASYNS010000212         3218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1763451, whole genome shotgun sequence.
ACCESSION   DASYNS010000212 DASYNS010000000
VERSION     DASYNS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1763451"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VGP50_06555"
     CDS             complement(<1..822)
                     /locus_tag="VGP50_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEV8027072.1"
                     /translation="MSVTGHDQLKARRVLKVDGREYDYFSLDAAGKAAGLGDVSRLPF
                     SMKVLLENLVRLENGRTVTLDDIKAVAEWLKTRSSEREIAFRPARVLMQDFTGVPAVV
                     DLAAMREAMAKLGGNPERINPLSPVDLVIDHSVMVDAFASSSAFAANVKLEFERNGER
                     YGFLKWGQTAFHNFRVVPPGTGICHQVNLEYLAQVVWTTEEGGRSIAYPDTLVGTDSH
                     TTMVNGLAVLGWGVGGIEAEAAMLGQPISMVLPEVVGFRLDGKLKEGATATDLVLT"
     gene            1014..3005
                     /locus_tag="VGP50_06560"
     CDS             1014..3005
                     /locus_tag="VGP50_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV8027073.1"
                     /translation="MALAQSELPARPMGQPDEALDTFPKLLLDHARRHGERPAMREKD
                     LGIWQSWSWRDAAREVQDFAAGLAALGFRRGDKLGIIGDNRPRLYWAIAAAQCLGGVP
                     VPVYQDAIASEMEFVLDHAEARFVLAEDQEQVDKLLAVKERLPRLEAIIYDDARGMRH
                     YAYAFLRSWDAVQEEGRRFAATRPRFLADAIAQGSGSDIAVILYTSGTTGQPKGVMLT
                     HRNVLSSAKSAAAREGLRADDEVLAYLPIAWAGDHIFSYAQSYATGFCVSCPESAGTV
                     MTDLRELGPTYFFAPPRIYENILTQVMIRMEDAGWAKRRLFHYFMALARRVGGRILDR
                     ETVSAKDRLLYALGDALIYGPLKNTLGFSRIRLAYTAGEAIGPDLFTFYRSLGINIKQ
                     LYAMTEASILCVQPDGEVKPDTVGTALPSVELLIADNGEVLYRGPAVFAGYYKNATAT
                     AAAKRPDGWVHSGDAGFLGDDGHLRIIDRAKDVGKLQDGTLFAPKFIENKLKFFPFIK
                     EAVAFGRDRQFVTAIINIDPGAVGNWAERNGITYGGYQELASLPAVYDLVQGCIEQVN
                     RDFAAESHLAGSQIRRFLILHKELEADDGELTRTRKVRRGFIGERYQPLVDALYADAP
                     TASIESEVTFEDGRRGTLKAELQIRDAKTAAAVAMLRRR"
     gene            3002..>3218
                     /locus_tag="VGP50_06565"
     CDS             3002..>3218
                     /locus_tag="VGP50_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8027074.1"
                     /translation="MSATRRIGAALLSAERISLSFGGVRALSDISIEIREREILAIIG
                     PNGAGKTSMLNVINGFYHPQEGTITFRG"
BASE COUNT          554 a         1067 c         1053 g          544 t
ORIGIN      
        1 ggtcagcacg aggtcggtgg cggtggcgcc ctccttgagc ttgccgtcga ggcggaagcc
       61 gacgacttcc ggcagcacca tcgaaatcgg ctgtcccagc atcgccgctt ccgcctcgat
      121 gccgcccacg ccccagccga ggacggcgag accattcacc atcgtcgtgt ggctgtcggt
      181 gccgacgagg gtgtcgggat aagcgatgct gcggccgcct tcctccgtcg tccacacgac
      241 ctgcgccagg tattcgagat tgacctggtg gcagatgccg gtgccgggcg gcacgacgcg
      301 gaaattgtgg aacgcggtct ggccccattt gaggaagccg tagcgctcgc cgttgcgctc
      361 gaattcgagc ttaacgttgg cggcgaaggc gctggaagac gcaaaggcat ccaccatcac
      421 cgagtggtcg atcacgagat cgacgggcga gaggggattg atgcgctcgg gattgccgcc
      481 gagcttggcc atcgcctcgc gcatcgccgc aagatcgacc acggcgggaa cgccggtgaa
      541 atcctgcatc agcacgcgcg ccggccggaa ggcgatctcg cgctcggagc tccgcgtctt
      601 cagccactcg gccaccgctt tgatgtcgtc gagcgtgacc gtgcgcccgt tctcgagccg
      661 caccagattc tcgagcagca ccttcatcga gaagggcagc cgcgagacgt cgccgagccc
      721 cgccgccttg ccggccgcgt cgaggctgaa atagtcgtat tcccgcccgt cgaccttgag
      781 gacgcggcgg gctttcagct ggtcatggcc ggtgacggac atggggcacc tcaggtcagg
      841 ctggacggat cgggggtgct gctacaactt tcgctcggga agatcaagag agcccgcatg
      901 cgttgcccgt tcgcctgctt gacccccgac atagcgatgc cgtagtggta gagcacagcg
      961 gcgcatgaca gaccagcaaa aaacgcaacg cgtgccgcgg gaggtttggg tcgatggctc
     1021 tggcgcagag cgagcttccg gcgcggccga tgggccagcc ggacgaagcg ctcgacacct
     1081 ttcccaagct gctcctcgac catgcgcgac gccatggcga gcgcccggcg atgcgcgaga
     1141 aggatctcgg catctggcag agctggagct ggcgcgacgc tgcgcgcgag gttcaggact
     1201 tcgccgccgg tcttgcggcg ctcggattcc gccggggtga caagctcggc atcatcggcg
     1261 acaaccgtcc caggctctac tgggcgatcg ccgcggcgca gtgcctgggc ggcgtgccgg
     1321 tgcccgtcta tcaggacgcc atcgcctccg agatggaatt cgtcctcgat catgccgagg
     1381 cgcgcttcgt cctcgccgag gaccaggagc aggtcgacaa actgctggcg gtgaaggaac
     1441 gcctgccgcg tctcgaggcg atcatttatg acgacgcccg cggcatgcgg cattacgcat
     1501 acgccttcct gcgctcctgg gacgcggttc aggaggaagg gcggcgcttc gccgcgacgc
     1561 gtccgcgctt cctcgccgac gcgatcgcgc agggcagcgg cagcgatatc gctgtcatcc
     1621 tctacacctc cggcaccacc ggccagccga agggcgtgat gctgacccat cgcaatgtgc
     1681 tctcctcggc gaagagcgct gccgcacgcg aggggctgcg cgccgacgac gaagtgctgg
     1741 cctatctgcc gatcgcctgg gccggcgatc acatcttctc ttatgcgcaa tcctatgcga
     1801 cgggattctg cgtgagctgc ccggaatcgg ccggcacggt gatgaccgat ctgcgcgagc
     1861 tcgggcccac ctatttcttc gcgccgccgc gcatctacga gaacatcctg acgcaagtga
     1921 tgattcgcat ggaggatgca ggctgggcga agcgccgact ctttcactat ttcatggcgc
     1981 tggcgcgccg cgtcggcggc cgcatcctcg accgcgagac ggtgtcagcg aaagatcggc
     2041 tgctctatgc gctgggcgat gcgctcatct acgggccgct caagaacacg ctgggcttca
     2101 gccgcattcg tctagcgtac acggcgggcg aggcgatcgg tcccgatctc ttcaccttct
     2161 atcgctcgct cggcatcaac atcaagcagc tctatgccat gacggaggcg tcgatcctct
     2221 gcgtccagcc cgatggcgag gtgaaacccg acacggtggg gacggcgctg ccgagcgtcg
     2281 agctcctgat cgccgacaat ggcgaggtgc tctaccgcgg ccccgcggtc ttcgccggct
     2341 attacaagaa cgcgacggcg acggcggcgg cgaagcgacc cgatggctgg gtgcattcgg
     2401 gcgatgccgg ctttttgggc gatgacgggc atcttcgcat catcgatcgc gcgaaagacg
     2461 tcggaaaatt gcaggacggc acgcttttcg cgccgaaatt catcgaaaac aagctgaagt
     2521 tctttccttt catcaaagaa gcggtcgctt tcggccggga tcggcaattt gtcacggcga
     2581 tcatcaacat cgaccccggc gccgtcggca actgggcgga gcgcaacggc atcacctatg
     2641 gcggctacca ggagctcgcg tctcttcccg ccgtctacga cctcgtccag ggctgcatcg
     2701 agcaggtgaa ccgcgacttc gccgccgagt cgcatctcgc gggatcgcag atccgccgtt
     2761 tcctcatcct gcacaaggag ctcgaggcgg atgacggcga gctcacgcgg acgcgcaagg
     2821 tgcgccgcgg cttcatcggc gaacgctacc agccgctcgt cgatgcgctc tacgccgacg
     2881 cgccgacggc gtccatcgag agcgaggtga ccttcgagga cgggcggcgc ggcacgctca
     2941 aggccgagct tcagatccgc gacgcgaaga ccgccgccgc tgtcgccatg ctgcgccggc
     3001 gatgagcgcg acgcggcgga tcggcgcggc gttgctcagc gccgaacgca tcagcctctc
     3061 cttcggcggc gtgcgcgcgc tttccgacat cagcatcgag atccgcgagc gcgagatcct
     3121 cgccatcatc ggtccgaacg gcgccggcaa gacctcgatg ctcaacgtca tcaacggctt
     3181 ctatcatccg caggaaggca cgatcacgtt tcgcggcg
//
LOCUS       DASYNS010000213         3657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3010630, whole genome shotgun sequence.
ACCESSION   DASYNS010000213 DASYNS010000000
VERSION     DASYNS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3657
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3010630"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /gene="ruvB"
                     /locus_tag="VGP50_06570"
     CDS             complement(<1..642)
                     /gene="ruvB"
                     /locus_tag="VGP50_06570"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV8027075.1"
                     /translation="MTGSRVVAPEPRRDDAAETFRPVALDEFIGQDPLRANLRIFVAA
                     ARARSEPLDHVLLSGPPGLGKTTLAQIIGREMGVGFRATSGPVIQRAGDLAALLTNLQ
                     PLDVLFIDEIHRLTPAVEEILYPAMEDFQLDLIIGEGPAARSVRIDLPPFTLVGATTR
                     SGLITRPLRERFGIPLRLVFYTPQELERIVARGARVLGMELDTEGAAEIARRSR"
     gene            complement(639..1232)
                     /gene="ruvA"
                     /locus_tag="VGP50_06575"
     CDS             complement(639..1232)
                     /gene="ruvA"
                     /locus_tag="VGP50_06575"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV8027076.1"
                     /translation="MIAMLAGVVDQRGADSLVLDVNGVGYLVFASARTLSRVPARGEP
                     LKLLIETHVREDHIHLYGFADEGERGWFRLLTTVQGVGARLALALLSVLAPDALATAI
                     MAQDRAALARADGVGAKLATRIASELKDKVGGIALAPVAATPATPVAATDAVSALVNL
                     GYSRSDAFAAVAQATQRLGAEAKIDALIRAGLQELAR"
     gene            complement(1229..2248)
                     /gene="ruvC"
                     /locus_tag="VGP50_06580"
     CDS             complement(1229..2248)
                     /gene="ruvC"
                     /locus_tag="VGP50_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEV8027077.1"
                     /translation="MRLLGLDPGLRHTGWGVVDAIGNRLSHVANGVVHSREGRPLAQR
                     LVDLFRQLNEVVDRYAPDEAAVEESFVNKNPASTLKLGVARGVVLLVPAERGLPVFEY
                     SANLIKKSVVGVGHADKQQIQMMVHRLLPGITLEAADAADALAVAICHAHHASTQRAW
                     AAGRLSAAAPVNTLSPSGRGKGEGVVAAALGKNTFLPGAAPSPLPSPRRGEGDLVAEA
                     RRKPSVVETERAKRLRRNATDPEMILWLRLRDRRFGGAKFRRQEAILEYTVDFVCHEQ
                     RLIIEVDGGQHGSRVAQDEHRTQRLEQAGFRVLRFWNNEVFDKLDGVLEVIGTALNTR
                     EGSAQ"
     gene            complement(2281..3030)
                     /locus_tag="VGP50_06585"
     CDS             complement(2281..3030)
                     /locus_tag="VGP50_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV8027078.1"
                     /translation="MAGHSQFKNIMYRKGAQDKKRAKIFTKIIRELTTAARTGLPDPA
                     ANPRLRAAVTAARDANMSKDTVERAIKRGAGNEEGENYEEVRYEGYGPGGVAIIVEAL
                     TDNRNRTASEVRAAFAKAGGHLGESNSVSFLFDRIGQVTYPAKAASADAMFEAAAEAG
                     ADNVESGAEEHVVTTAPDALNAVRDVLEQRFGAPAAAKLVWKPKTQSLVEAMVADALF
                     KLLETLEDSDDVQNVFANFEVSDDVMARLSA"
     gene            complement(3172..>3657)
                     /locus_tag="VGP50_06590"
     CDS             complement(3172..>3657)
                     /locus_tag="VGP50_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEV8027079.1"
                     /translation="IWAAAVALGAGALLDFARLRLLLSIVSSALLLLLGALFLRRAAR
                     GAADWRRGVEAQTSGRFEGGAAGYLLGIGLALSSPWNVAFWLAVLGAPDTLQAGFAGA
                     LVVAGSVLLGTVTWVLLLSGAAALLGLRFMSPLWTLAASGASGLLMLLFAARGIARLL
                     G"
BASE COUNT          617 a         1253 c         1214 g          573 t
ORIGIN      
        1 gcgcgagcgc cgcgcgatct cggcggcgcc ttcggtgtcg agctccatgc cgagcacgcg
       61 cgcgcctcgg gccacgatcc gctccagctc ctgcggcgtg tagaagacga ggcgcagcgg
      121 gatgccgaag cgttcgcgca acggacgcgt gatgagcccc gagcgcgtcg tcgccccgac
      181 cagagtgaaa ggcggcagat cgatccggac cgaccgcgcc gccggtcctt cgccgataat
      241 gaggtcgagc tgaaagtcct ccatggccgg atagaggatc tcctcgaccg cgggcgtcag
      301 ccgatggatc tcgtcgatga acaaaacgtc gagcggctgc agattggtga ggagcgccgc
      361 gaggtcgccg gcgcgttgga ttaccggacc cgaagtggcg cggaacccga cccccatctc
      421 gcggccgatg atctgcgcga gggtcgtctt gccgaggccg ggcggcccgg acagcagcac
      481 gtgatcgagc ggctcgctcc gtgcgcgcgc agcggcgacg aagatgcgca gattggcccg
      541 caacgggtcc tgaccgatga actcgtcgag agcgaccggt cggaacgtct cggccgcgtc
      601 gtcgcgacgc ggttcgggtg ccaccacgcg agagccggtc atcgcgcaag ctcctggagc
      661 ccggcgcgga tcagggcatc gatcttcgcc tcggcgccca gccgctgcgt cgcctgcgcc
      721 acggcggcga aggcgtcgct gcgtgaatag ccgagattga ccagggccga cacggcgtcg
      781 gtcgcggcca ctggcgtggc cggcgtcgcc gccaccggtg cgagagcgat gccgcccacc
      841 ttgtccttga gctcgctggc gatacgcgtg gcgagcttgg cgccgacacc gtccgcgcgc
      901 gccaaggccg ccctgtcctg cgccatgatg gccgtggcaa gcgcatcggg agcgaggacg
      961 ctcaaaagcg ccagcgccag ccgcgcgccc acgccctgca ccgtggtcag aagccggaac
     1021 cagccgcgct cgccctcgtc ggcgaagccg tagagatgga tgtggtcctc gcgcacatgc
     1081 gtctcgatca ggagcttcag cggctcgccg cgcgccggca cgcgactcaa ggtgcgcgcc
     1141 gatgcgaaga cgagatagcc cacgccattc acgtccagca cgaggctgtc cgcgccccgt
     1201 tgatcgacga cgccggcgag catggcgatc attgcgcgct cccctcgcgg gtgttcagcg
     1261 ctgttccaat gacctcgagg acgccgtcga gtttgtcgaa cacttcgttg ttccagaaac
     1321 gcagcacacg gaaaccggct tgctcgagcc tctgcgtgcg gtgctcgtcc tgagcaactc
     1381 ttgagccgtg ttgaccaccg tcgacttcga tgatcagacg ttgttcatgg cagacgaaat
     1441 cgacggtgta ttccaatatc gcttcctggc ggcgaaattt tgcaccgccg aaccgacgat
     1501 cgcgcagcct cagccacaga atcatttccg ggtcggtcgc attgcgtcgc aaccgcttgg
     1561 cccgctcagt ctccactaca gatggcttgc gtcgcgcctc agcaaccaaa tccccctctc
     1621 ctctccgggg agagggcagg ggtgaggggg cggcgccggg gaggaatgtg tttttaccga
     1681 gtgcagcggc aaccaccccc tcacccttcc ctctcccgga aggggagagg gtattgacgg
     1741 gggcggctgc agagagacgc ccggcggccc aggcgcgctg ggtcgacgcg tgatgggcat
     1801 ggcagatggc gacggcgagc gcgtccgccg cgtcggccgc ctcgagcgtt atgcccggca
     1861 acaggcggtg caccatcatc tggatctgct gcttgtcggc atgaccgacg ccgacgaccg
     1921 atttcttgat caaattcgcc gaatactcga agaccgggag gccacgctcc gccggcacca
     1981 gcagcacgac accccgcgcc acgccgagct tcaaggtcga ggccggattc ttgttgacga
     2041 agctctcctc caccgccgcc tcgtccggcg cgtagcggtc gaccacttcg ttcagctggc
     2101 gaaagaggtc gaccaggcgc tgcgcgaggg gccgaccttc gcgcgaatgg acgacaccgt
     2161 tggcgacatg gctgaggcga ttgccgatcg catcgaccac gccccagccg gtgtgacgga
     2221 gccccggatc aagtccgagc aggcgcatgg gatgtccttg gtcgattcct gccggcgaaa
     2281 tcaggcgctc agccgcgcca tgacgtcgtc ggacacctcg aagttcgcga acacgttctg
     2341 aacgtcgtcg ctatcctcca gcgtctcgag gagcttgaac aaagcgtcgg ccaccattgc
     2401 ctcgacgagg ctctgcgtct tcggcttcca cacgagcttc gccgcggccg gcgcgccgaa
     2461 gcgctgctcg agcacgtcgc gcacggcatt gagcgcatcg ggcgcggtgg tcaccacatg
     2521 ctcttcggct ccgctttcga cattgtcggc accggcttcc gccgccgctt cgaacatggc
     2581 gtcagccgaa gccgccttgg cgggataggt cacctggccg atgcgatcga aaagaaagct
     2641 gacgctgttg ctctcgccga gatggccgcc ggccttggca aaagccgcgc gcacctcgct
     2701 cgccgtgcgg ttgcgattgt ccgtgagcgc ctcgacgatg atcgccacgc cgccgggacc
     2761 atagccctcg tagcggactt cctcgtagtt ctcgccctct tcgttgccgg cgccgcgttt
     2821 gatcgcgcgc tcgaccgtat ccttcgacat gttcgcgtcg cgcgcggccg tgactgcagc
     2881 gcgcaagcgc ggattcgctg cggggtcggg caagcccgtg cgggcggcag tggtcagctc
     2941 gcggatgatc ttggtgaaga tcttggcgcg cttcttgtcc tgcgcgccct tgcgatacat
     3001 gatgttcttg aactgggaat ggccggccat gattgatcgc tggttcctga aatttctggc
     3061 ttcggctcgg gaccatacca catctgctga cggcacgaac gcggcgcatc gcagggcgtt
     3121 gcggccgcgt atcccggcgt cttagcggct caatatggca gcattacggc gctagccaag
     3181 caatcgggcg atacccctcg ctgcgaagag gagcatgagg agcccgctcg cgccgcttgc
     3241 cgccagggtc cagagcgggc tcatgaagcg caggccgagc aatgctgcgg cgccggagag
     3301 cagcaggacc caggtgaccg tgcccagcag caccgagccc gccaccacca gcgcgcccgc
     3361 gaagcccgcc tgaagtgtgt ccggcgcgcc caggaccgcg agccagaaag cgacgttcca
     3421 cgggctcgac aaggcgaggc cgatgccgag caaatagccg gcggcaccac cctcgaatcg
     3481 gccggaagtc tgcgcctcga cgccccggcg ccagtcggcg gcgccccgtg ccgcgcggcg
     3541 caggaacaac gctccgagca agagcagcag tgccgaactg acgatggaga gcagcagccg
     3601 gagccgggcg aaatcgagca gcgccccggc gcccagcgcc accgccgcgg cccagat
//
LOCUS       DASYNS010000214         7721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4416478, whole genome shotgun sequence.
ACCESSION   DASYNS010000214 DASYNS010000000
VERSION     DASYNS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4416478"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VGP50_06595"
     CDS             complement(<1..566)
                     /locus_tag="VGP50_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEV8027080.1"
                     /translation="MTLLPRHPAPPPHRRRTWAALLVIGVAAAVPILMAPAGRGARAT
                     IDRCAAPPDLAALGAPLPRTAARLDARRPLTIVALGSSSTEGVGATQPALSYPSRLSV
                     WLEARFPGVPIRVINRGVGGEMTPQMLARLDRDVLAEKPDLVIWQLGTNGVLHDVDPA
                     DETELARRGIERIKAAGADVILMDLQYAP"
     gene            complement(563..1297)
                     /locus_tag="VGP50_06600"
     CDS             complement(563..1297)
                     /locus_tag="VGP50_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEV8027081.1"
                     /translation="MRLAVLLAGIFVAVHTVPARAAAADCSVPAELVEDEPRLPALAE
                     QLRQKHPITIVAIGGATTAGMAAGNGADSAYPRRLEDALRRRHPDVPITVINKGVSRQ
                     TAQQMVDRFTRDVYAFAPTLVIWETGTFDAAEGEELDGFASALEAGLADLRERQVDTM
                     LMNMQYSRTTASVINFEPYLDAMQQTADVADVYLFRRFEMMKYWSESGVFNFANVPPE
                     GRAPLAAAVYECIAQRLADAIEYAVK"
     gene            complement(1306..2490)
                     /locus_tag="VGP50_06605"
     CDS             complement(1306..2490)
                     /locus_tag="VGP50_06605"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HEV8027082.1"
                     /translation="MATTISAKDDGAASAVPRKGRDLRLDFFRGLSLFFIFIDHIPAN
                     FLGYFTLRSIAFSDAAEVFIFISGYTAALVYGRSLYQRGALFATAQIWRRVWQLYVAH
                     IFLFVIFTAEVSYAVLAVNNPMYSEEMGVGDFLQQPHVAIIRALLLQFQPTFLDILPL
                     YITLLAIFPLVLIALTRGPLWALLPSAAVYALTLRHGWALPGYPADHVWFFNPLAWQF
                     LFIIGASAGHARVTGRWPFPHGEWLSQLAVVFAAALVVINVSWAIHWVYDPFPALLER
                     PLYDLTTDKTDLAPLRLLNFLALALATVYFVRQDSGFLRWRVAHPIILCGQHSLYIFC
                     LGIILAVLGHFVLSEFYGTLAMQIVVNLAGFGLMVGTAALLAWYKSAGMTGSSVPAMP
                     SE"
     gene            complement(2599..2913)
                     /locus_tag="VGP50_06610"
     CDS             complement(2599..2913)
                     /locus_tag="VGP50_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027083.1"
                     /translation="MAFLYGRPIWSRCAMGALAAALVAALPVAQSAAQRAAANSADSP
                     PDRAAGAPEANSPPGAEGAKSGESAPATVGESPQERDRRLVLLRLLVMGGGNYRPFGF
                     FR"
     gene            complement(3207..4007)
                     /locus_tag="VGP50_06615"
     CDS             complement(3207..4007)
                     /locus_tag="VGP50_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027084.1"
                     /translation="MQRPSDERLIAYLDGELAESERADIALWLERDAALRERAARLTE
                     SAALLRSAFDDVLHEALPERLVEAAHGTSAGGAVVDFVAVRDARAARRGGRRWWMGMA
                     VAASLFGLMVGAGATIVWGTHGDPFNDAELAADTYFPDNLAGYYKRYVNVAPEDAVAF
                     DKLPQNFSVPNLKPWGLDFQGARFLMVEGQPATALLYGTNDKTLGPVIVVVANTGKSD
                     SQGGPDTRGDINVWRWVHHGHAYAVAGTANTNYLWNLHNDLAYQFDGI"
     gene            complement(3991..4632)
                     /locus_tag="VGP50_06620"
     CDS             complement(3991..4632)
                     /locus_tag="VGP50_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV8027085.1"
                     /translation="MSAMLDRAMIAAGNKAHHQPLFSRQVSVGGPPPSESGAVSVSIN
                     DQLIAVLPRLRRFARGLAGSAVEADDLVQAACERALTRAHQFQEGTRFDSWMFRIVQT
                     IWIDQLRSRDTRREEAEEHGVNVGTDEPGRRVEARLALQEVRTALEELPLEQRAALLL
                     VTVDGLSYKEAAEVADVPVGTIMSRLARARVALQAKLDAGGGLRRSTNDAKAK"
     gene            4770..5933
                     /locus_tag="VGP50_06625"
     CDS             4770..5933
                     /locus_tag="VGP50_06625"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEV8027086.1"
                     /translation="MKISIPKERRPQERRVAASPDSVKRMVGMGLEVLVESGAGAGSS
                     FPDQAYEAAGAKIAADAKAALGEGDIVLKVQRPLHSGDGALDELALMKRGAVLIGMLQ
                     PLQYRQDVEAYARAGITAFAMELVPRITRAQAMDVLSSQANLAGYRAVLDAAGEFGRA
                     FPMMMTAAGTVAPARVLVMGAGVAGLQAIATARRLGGVVSATDVRLAAKEQVESLGAT
                     FLVVDVEAMKAMETAGGYAKEMGEDFARRQREHVTEALKKTDIVICTALVPGRKAPVL
                     ITKAMVAVMRPGSVIVDLAVEQGGNVEGSEPGKIVTTANGVTIVGHANVPSRIAVDAS
                     QLYARNLLAFLALLIDKDKKLKIDTADEIVKATLLTRDGQVVNPQLAPAAAGA"
     gene            5962..6234
                     /locus_tag="VGP50_06630"
                     /pseudo
     CDS             5962..6234
                     /locus_tag="VGP50_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
     gene            6249..7643
                     /locus_tag="VGP50_06635"
     CDS             6249..7643
                     /locus_tag="VGP50_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEV8027087.1"
                     /translation="MSANISALLYLVASVCFIMALRGLSSPDTARAGNLYGMVGMAIA
                     VGTTLALPGIVSYPVIALGVIIGGLIGAGVASRIQMTALPQLVAAFHSLVGLAAVCVA
                     TAAFYDPDAYAIGMPGAIHASSLVEMSLGTAIGAITFTGSIIAFGKLQGLITGKPLVF
                     PGQHPLNALLGLALVAIIVWFVIGQPAAAFWAIVAVSLLLGLLLIVPIGGADMPVVIS
                     MLNSYSGWAACGIGFTLANSLLIIIGALVGSSGAILSYIMCKGMNRSIFNVILGGFGT
                     DGGTVAAGAHADRGVKSGSAEDAAFIMKNASSIIVVPGYGMAVAQAQHALREMADILK
                     REGAQVRYAIHPVAGRMPGHMNVLLAEANVPYDEVLELEEVNRDFASTDVAFVIGAND
                     VTNPAAKTDPKSPIYGMPILDVEKAKTVLFVKRSMASGYAGVENELFYRDNTMMLFGD
                     AKKMCEEIVKALGA"
BASE COUNT         1378 a         2545 c         2529 g         1269 t
ORIGIN      
        1 ggcgcgtatt gcagatccat gagaataacg tcggcgcccg ctgccttgat gcgctcgatg
       61 ccgcggcgtg cgagctcggt ttcatccgcc gggtcgacgt cgtgaaggac gccgttggtg
      121 ccgagctgcc agatcacaag gtccggcttc tccgccagaa cgtcgcgatc gaggcgcgcc
      181 agcatctgtg gggtcatctc gccgccgact ccacggttga tgacacggat cggcacgccc
      241 gggaagcgcg cctcgagcca aaccgagaga cggctcggat aactgagcgc gggctgcgtc
      301 gcacccacgc cctcggtcga ggacgagccc agcgccacga tggtgagcgg gcgacgggcg
      361 tcgaggcgcg cggccgtacg cggcaacggc gcacccaatg cggcgaggtc cggcggagcc
      421 gcgcaacggt cgatggtcgc ccgcgcgcca cggcccgccg gcgccatgag aatgggtacc
      481 gcggcggcga cgccgatcac caggagcgct gcccaggtgc ggcggcgatg cggtggcgga
      541 gcgggatggc gcggaagcaa tgtcatttca ccgcatactc gatcgcgtcg gcgaggcgtt
      601 gtgcaatgca ttcataaacg gcggcagcca gcggtgcgcg cccctcgggc ggcacattgg
      661 cgaagttgaa cacgccgctc tcgctccagt acttcatcat ctcgaaacgc cggaacagat
      721 agacatccgc cacgtccgcc gtttgctgca tcgcatcaag atacggctcg aaattgatca
      781 cgctagctgt agtgcgacta tattgcatat tcattaacat tgtgtcgacc tgccgctcgc
      841 gcaaatcggc gaggccggct tcgagagcgc tcgcaaatcc gtccagttcc tccccctccg
      901 ccgcatcgaa agtgccggtc tcccaaatca ccagcgtcgg cgcaaaggcg tagacgtcgc
      961 gcgtgaagcg atcgaccatt tgttgcgcag tctggcgcga cacccctttg ttgatcaccg
     1021 tgattggaac atcgggatgg cgccgccgca atgcgtcctc gaggcgacgt ggataggcgc
     1081 tgtcggcgcc gtttcccgcg gccatgcctg ccgtggtcgc tccgccgatg gcgacgatgg
     1141 tgatcggatg cttctggcgc agctgctccg ccagcgccgg caaccgcggc tcgtcctcca
     1201 ccaactccgc cggaacgctg caatcggccg cggcggcgcg cgccggcacg gtgtgcactg
     1261 caacgaaaat gccggcaagg agcacggcca ggcgcatgat ctccatcact ccgacggcat
     1321 tgccggcacg gaagagccgg tcatgccggc gctcttgtac caggcgagca gggcggccgt
     1381 gcccaccatg aggccgaagc ccgccaggtt cacgacgatc tgcatcgcca gcgtgccata
     1441 aaactcggac agcacgaagt gaccaagcac ggcgaggatg atgccgagac agaagatgta
     1501 gagcgaatgc tgaccgcaga ggatgatcgg gtgcgccacg cgccagcgca ggaaaccgct
     1561 atcctgccgc acgaaataga ccgtggcgag cgccagcgcc aggaaattga gcagtcgcag
     1621 gggcgcgagg tcggtcttat ccgtggtgag atcgtagagc ggcctttcca gaagcgcggg
     1681 gaagggatcg tagacccaat ggatcgccca gctcacattg atgacgacca gcgccgcagc
     1741 gaaaacgacg gccagttgcg agagccattc gccatgcggg aagggccacc gtcccgtgac
     1801 gcgggcatgg ccggcgctcg cgccgatgat gaacaggaat tgccaggcca gcgggttgaa
     1861 gaaccacaca tggtcggcgg gatagccggg gagcgcccag ccatggcgaa gcgtcagcgc
     1921 atagaccgcc gccgacggca gcaacgccca gagcggccca cgcgtcagcg cgatcagcac
     1981 cagcgggaag atggcgagca gcgtgatgta gagcggcagg atatcgagga aggtcggctg
     2041 gaactgcagc aacagcgcgc ggatgatcgc gacatgcggc tgctgcagga aatcgccgac
     2101 gcccatctcc tcgctgtaca tcgggttgtt gacggcgagc accgcgtagg atacttcggc
     2161 cgtgaaaatg acgaaaagga agatgtgagc gacatagagc tgccagacgc ggcgccagat
     2221 ctgcgccgtg gcgaagagcg cgccccgctg atagaggctc cggccgtaga ccagcgccgc
     2281 cgtatagccc gatatgaaga tgaacacctc ggcggcatcc gagaaggcga ttgatcggag
     2341 cgtgaaatag ccgaggaaat tcgccgggat atgatcgatg aagatgaaga acagcgagag
     2401 gccgcggaag aaatcgagcc gcaggtcgcg ccctttgcgc ggcacggcag aggccgcgcc
     2461 gtcgtccttg gccgaaatcg tcgtcgccat gggacgccat catagacggc gcgcgtgctc
     2521 aatgctcgcg ggacgccggg cgtgacgatg ggtcgcgatc gctgaggatc gcgcgcccga
     2581 tcctgttccc gccgcgcgtt atctgaagaa accgaagggg cgatagttac cgccgcccat
     2641 caccaggagg cgaagcagga cgaggcggcg gtccctttcc tgcggactct cgccgaccgt
     2701 agccggcgcg gactcgccgc tcttcgcccc ttcggcgccg ggcgggctgt tggcctcagg
     2761 cgctcctgcg gcccggtcgg gcgggctgtc cgcggagttt gccgctgcgc gttgcgccgc
     2821 gctctgggcg accggcaacg cggcgacgag tgccgccgcc aatgccccca tcgcgcatcg
     2881 cgaccagatc ggtcgaccgt aaaggaaagc catggcttgg ctcctagtta tagattttta
     2941 tcgccgagcg ccgactagta atagcgttgg ttcgccgaac ctcggcgaag caacgaagcc
     3001 caatcgggaa ccacggccgc cttgcgcaaa agggagcagc tattgtcgat ttgctgcgaa
     3061 acgctggcgg ttgtggaccc caccccacaa acttcgacaa gactaccacg tttcgcggtt
     3121 aaattactct taatcaaaaa atatttgccg tcgggggcct gctgaaagcg caagccgccg
     3181 cggctgaccg cgggccgaaa gggacctcag ataccgtcga actggtaggc gaggtcgttg
     3241 tgcaggttcc agagatagtt ggtgttggcg gttccggcga ccgcataggc gtggccgtga
     3301 tggacccagc gccagacatt gatatcgccg cgcgtgtcag gtccgccctg cgagtcggac
     3361 ttgcccgtat tggccaccac gacgataacc ggtccgagcg tcttgtcgtt cgtcccataa
     3421 agaagcgccg tcgccggctg gccctccacc atgaggaaac gggcgccctg gaagtcgagg
     3481 ccccagggct tgaggttcgg cacggagaaa ttctgcggca gcttgtcgaa cgcgaccgcg
     3541 tcctccggcg cgacattgac atagcgcttg tagtagccgg cgagattgtc cgggaaatag
     3601 gtgtcggcag ccagctcggc atcattgaag ggatcgccat gcgtgcccca gacgatcgta
     3661 gcaccggccc cgaccatcag gccgaagagc gacgcggcga cagccattcc catccaccag
     3721 cgccggccgc cgcggcgcgc cgcgcgcgca tcgcgcaccg ccacgaaatc gaccaccgca
     3781 ccgccggcgc tcgtgccatg cgcggcctcg acgagccgct cgggcagcgc ctcatgcagg
     3841 acgtcgtcga aggcactgcg cagcagcgcc gcgctctcgg tgagccgcgc cgcgcgctcg
     3901 cgcagcgccg cgtcccgctc gagccaaagc gcgatgtcgg cgcgctcgct ctcggcaagc
     3961 tcgccgtcga gataggcgat cagcctttcg tcacttggcc tttgcatcat tcgtgctcct
     4021 ccgaaggccg ccgccggcat cgagcttggc ctgaagcgcg acacgggccc gcgccagacg
     4081 gctcatgatc gttcctacgg gtacgtcggc gacctccgct gcttccttgt aactcaatcc
     4141 atccaccgtc acgaggagga gtgccgcccg ttgctccagt ggcagctcct cgagcgcggt
     4201 ccgcacttcc tgcaaggcca accgcgcctc gacgcgtcgt cccggctcgt ccgtgccgac
     4261 attcacgcca tgctcctcgg cctcctcgcg ccgcgtgtcc cgcgagcgca gctgatcgat
     4321 ccagattgtc tgcacgatgc ggaacatcca gctgtcgaaa cgagtcccct cctgaaactg
     4381 gtgcgcccgc gtcagcgccc gctcgcacgc ggcctggacg agatcgtcgg cttcgacggc
     4441 ggagccggcc aagccgcggg cgaagcgccg caaacggggc aggacggcga tgagctggtc
     4501 attgatcgac acgctcaccg cgccgctctc cgaaggaggc gggccaccca cgctaacctg
     4561 acgggaaaac aacggctggt gatgggcttt attccctgca gcgatcatgg cgcggtcaag
     4621 catcgccgac attttagaga gccggagccg ccctgaaaag gctcgccgcc ctgccgctgg
     4681 cggccctcaa atttggtgga aatgcttcac aaatccctaa cgccacggca ttgtgtcgcg
     4741 cttgctccgg gggaggggac gtcgtctcga tgaaaatcag tattcccaag gaacgccgtc
     4801 cgcaggagcg gcgggtggcg gcctcgccgg acagcgtcaa gcgcatggtc ggcatgggcc
     4861 tcgaggtgct ggtggagagc ggtgccgggg cggggagctc gtttcccgat caggcctacg
     4921 aggccgcggg cgccaagatt gcggccgacg ccaaggcggc gctcggcgaa ggcgacatcg
     4981 tcctcaaggt gcagcgtccc ctgcattcgg gcgacggcgc cctcgatgag ctggcgctca
     5041 tgaagcgcgg ggcggtgctg atcgggatgc tgcagccgct tcagtatcgc caggatgtcg
     5101 aggcctatgc acgcgcaggt atcacggctt tcgccatgga gctcgttccg cgcatcaccc
     5161 gcgcccaggc gatggacgtg ctctcttcac aggcgaacct cgccgggtat cgcgccgtgc
     5221 tcgatgccgc gggcgagttt ggacgcgcct ttccgatgat gatgacggcg gccggcaccg
     5281 tggcgccggc gcgtgtgctg gtgatggggg caggcgtcgc cgggctccag gccatcgcca
     5341 cggcgcggcg cctgggcggc gtcgtctccg ccaccgatgt gcggctcgcc gccaaggagc
     5401 aggtggagag cttgggcgcc accttcctcg tcgtcgatgt cgaggcgatg aaggcgatgg
     5461 agacggccgg cggctatgcc aaggaaatgg gcgaggattt cgcccgacgc cagcgcgagc
     5521 atgtcaccga agcgctcaag aagaccgaca tcgtcatctg cacggcgctc gttcccggac
     5581 ggaaggcgcc ggtgctgatc accaaggcga tggtggcggt gatgcggccg gggtccgtga
     5641 tcgtcgatct cgcggtcgag cagggcggca atgtcgaagg aagcgagccc ggcaagatcg
     5701 tgacgacggc gaatggcgtg acgatcgtcg gccatgccaa cgtgccgtcg cgcatcgccg
     5761 tcgatgcgag ccagctttat gcccggaacc tgctcgcttt cctggcgctg ctcatcgaca
     5821 aggacaagaa gctgaagatc gacacggccg acgagatcgt caaggcgacg ctcttgaccc
     5881 gcgacgggca ggtcgtcaac ccacaactgg cgccggccgc ggcgggagca tgaggccatg
     5941 gagcacaccg atgtcttcgt cttttttctc accgtcttcg tgctggcttg cttcgtcggc
     6001 tactatgtcg tttggcgggt gacgccggcg ctgcattcgc cgctgatgag cgtcaccaac
     6061 gccatctcga gcgtgatcat cgtgggcgct ctcatcgccg ccggtcccgc cggcctgagc
     6121 ttctcgaagg tgatggggtt cgtcgcggtc attctcgcct cggtgaacat cttcggcggc
     6181 ttcatcgtca cgcagcgcat gctgcagatg ttccagaaga agaaacccaa gtaagggagc
     6241 gccgcttcgt gagcgccaac atctcggctc tgctctatct cgtcgcctcc gtctgcttca
     6301 tcatggcgct gcgtgggctc tcctcgccgg atacggcgcg cgccggcaat ctctacggca
     6361 tggtcggcat ggcgatcgcc gtgggcacga ccctcgcgct ccccggcatc gtcagctatc
     6421 ccgtcatcgc gctcggcgtc atcatcggcg gcctcatcgg cgcgggcgtc gcctcgcgca
     6481 tccagatgac ggcactgccc cagctcgtcg ccgccttcca cagcctggtc ggtctcgccg
     6541 ccgtctgcgt cgccaccgct gccttctacg accccgacgc ctacgccatc ggcatgccgg
     6601 gcgcgatcca tgccagcagc ctcgtcgaga tgtccctcgg cacggcgatc ggcgccatca
     6661 ccttcacggg ctcgatcatc gccttcggca agctgcaggg cctgatcacc ggcaagccgc
     6721 tcgtcttccc cggccagcat ccgctcaacg ccctcctcgg cctggctctc gtcgcgatca
     6781 tcgtttggtt cgtgatcggg cagccggccg cggcgttctg ggcgatcgtc gccgtctcgc
     6841 tgctgctcgg cctcctgctc atcgtgccga tcggcggcgc cgacatgccg gtggtcatct
     6901 cgatgctcaa ctcctattcc ggttgggccg cctgcggcat cggcttcacg ctcgccaaca
     6961 gtctcctcat catcatcggc gcgctggtgg gatcctcggg cgcgatcctc tcctacatca
     7021 tgtgcaaggg gatgaaccgc tcgatcttca acgtcattct cggcggcttc ggcaccgacg
     7081 gcggcaccgt ggcggcgggc gcccatgccg accgcggcgt caagtcgggg agcgccgagg
     7141 atgccgcctt catcatgaag aatgcgagct cgatcatcgt cgttcccgga tatggcatgg
     7201 cggtggctca ggcgcagcat gcgctccgcg agatggccga catcttgaag cgcgaggggg
     7261 ctcaggtgcg ctatgccatc catccggtgg cggggcgcat gcccggccac atgaatgtgc
     7321 tgctcgccga agccaacgtc ccctatgacg aggtgctgga gctcgaggag gtcaaccgcg
     7381 acttcgcctc gaccgacgtc gctttcgtga tcggtgccaa cgacgtcacc aaccccgcgg
     7441 ccaagacaga ccccaaatcg cccatctatg gcatgccgat cctcgacgtc gaaaaggcga
     7501 agaccgtgct cttcgtcaag cgctcgatgg cgtcgggcta tgccggcgtc gagaacgagc
     7561 tcttctatcg cgacaacacg atgatgctgt tcggcgacgc caagaagatg tgcgaggaaa
     7621 tcgtcaaggc gctgggcgct taaccgcgaa cccgagtaga aagtatcacc agacaggcgg
     7681 tgaggcagcg agaaggagga atgacgcgct ccgcgcgccc a
//
LOCUS       DASYNS010000215        10508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2543594, whole genome shotgun sequence.
ACCESSION   DASYNS010000215 DASYNS010000000
VERSION     DASYNS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2543594"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VGP50_06640"
     CDS             <1..333
                     /locus_tag="VGP50_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8027088.1"
                     /translation="LILVPFAPSAGIAVALLMARSLLSQMDVPTRTSYVMAVVTPGER
                     AAAASVTSVPRSLAAAVGPIIAGWLLALSPFGWSLVAAGALKSIYDLLLLMLFRGHKP
                     PEETERTR"
     gene            complement(449..1294)
                     /gene="hemF"
                     /locus_tag="VGP50_06645"
     CDS             complement(449..1294)
                     /gene="hemF"
                     /locus_tag="VGP50_06645"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEV8027089.1"
                     /translation="MTEALQDRVARWFNELRDSICAAFEALETEYVQRRPDEGAPGRF
                     ERKPWSREGGGGGTMATLRGRVFEKVGVNVSAVWGEFSSDFRQQIPGAAEDPRFWAAG
                     ISLVAHMRSPLVPAVHMNTRHIRTTKGWFGGGSDLTPIYPDAADTQAFHAALQAACDA
                     HDPAYYPRFKKWCDEYFFLPHRNESRGVGGIFFDYLDSGDPERDFTFLRAIGKAFLAI
                     YPAIVRRHMWEAWTPEQRAHQLMRRGRYVEFNLLHDRGTLFGLKTGGNVEAILMSLPP
                     EVAWP"
     gene            complement(1291..1749)
                     /locus_tag="VGP50_06650"
     CDS             complement(1291..1749)
                     /locus_tag="VGP50_06650"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV8027090.1"
                     /translation="MRLALYEPDIPQNAGALLRLGACLGVAVDIIGPCGFVLSDARFR
                     RAGMDYLDHATLARHDSWAAFLAQNQTRLVLLTTRGDLAYHRFRFRADDTLLLGRESA
                     GVPEAVHQAADARLRIPLRPETRSLNMALAAAMVLGEALRQTEQFPKERG"
     gene            1950..2510
                     /gene="petA"
                     /locus_tag="VGP50_06655"
     CDS             1950..2510
                     /gene="petA"
                     /locus_tag="VGP50_06655"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEV8027091.1"
                     /translation="MAEAAQARPMDPHTQDSPTRRDFLTIAATALAGVGAAIAVWPLI
                     DSMNAAKDTLALSTTEVDLAPVQLGQRLTVKWRGNPVFIDHRTPDEIKAADAVDIATL
                     RDPQTDGARVQKAEWLIVVGVCTHLGCVPLGQKATDNRGEYGGWFCPCHGSQYDTSGR
                     IRQGPAPLNLAVPKYEFTTDTSVKIG"
     gene            2523..3839
                     /locus_tag="VGP50_06660"
     CDS             2523..3839
                     /locus_tag="VGP50_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6"
                     /protein_id="HEV8027092.1"
                     /translation="MAARPPQNKIIRWLDYRLPIFGMLHHELYEYPTPKNLSYWWNFG
                     SLAGIMLLIMIITGIFLAMQYTPHVDYAFNSVERIRRDVNFGWLIQYIHMNGGSFFFI
                     VTYIHVFRGLYYGSYKAPRELLWMLGVLILLLMMATAFMGYVLPWGQMSFWGAKVITS
                     LFSAIPVVGDYIVHWLWGGFAVDNPTLNRFYALHYLLPFIIVAVVMLHLIALHTHGSN
                     NPLGIDTKGPQDSLPFHPYYTFKDMFGLAVFLVAYCIFIFYMPNALGDPQNYIPANPL
                     QTPPEIVPEWYFLPYYAILRSVPNILFIPAKLAGVMCLFGAIGVLFILPWLDTSPVRS
                     ARFRPIYKWLFWVLLIDVVVLGIVGAHRPDLIVLSVNVLGLDVVVTYLVIGQIATLYY
                     FVHFLLLVPLIGWIEQPLPLPTSISVAVAKPGGGRLVGASTPMEKP"
     gene            3839..4678
                     /locus_tag="VGP50_06665"
     CDS             3839..4678
                     /locus_tag="VGP50_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEV8027093.1"
                     /translation="MRQALTVFGGVIIAVVLMALIIASYRHPAPTSAGVIEEPQPVLP
                     HQKWSFDGPFGTYDRAAVQRGFQVYSEICSQCHSMTLMHYGDLGPGGPGGGIGYTEDE
                     VKAIAATPFVNAKQVTDGPNDQGQMFQRPGRPSDKFVAPFDHPEEAAAAYGAIPPDLS
                     VIVKARAGGADYVFGLLTGYKDEPPPGVKMAAPNLQYNEYFPGHQIAMPPPLEDNRVT
                     YADGTKATLDQEARDIATFLSWASEPIMEERKRDGAKVLIFLAVMVIVFFFAKRKVWA
                     DVH"
     gene            4864..5742
                     /locus_tag="VGP50_06670"
     CDS             4864..5742
                     /locus_tag="VGP50_06670"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEV8027094.1"
                     /translation="MASDAVIGIIGGSGLYQIDGLTEVEWRRVASPFGEASDELCFGK
                     LGGTRVVFLPRHGRGHRLPPSEINYRANIDVLKRAGATDLLSLSAVGSLKADLAPGTF
                     LLVDQFIDRTFAREKSFFGTGLVAHVGFGHPVCARLGDALAAAGKDAGLAMPRGGTYV
                     CMEGPQFSTLAESNLHRSWGCAVVGMTNMPEAKLAREAELCYASVAMVTDFDCWHPDH
                     DHVTVDMIIGVLTANAERAKGLVKAVVPLLAQHRGACPHGCRNALDNALITSVEARDP
                     ALVAKLDAIAGRALKR"
     gene            complement(5747..5947)
                     /locus_tag="VGP50_06675"
     CDS             complement(5747..5947)
                     /locus_tag="VGP50_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027095.1"
                     /translation="MNSVKIFTVSGNQQVAKLEGDINEWLQTLAADVEVKLTDVVLDR
                     VSNNQTGGEDRRLMVMVWTGQR"
     gene            6054..7166
                     /gene="mtnA"
                     /locus_tag="VGP50_06680"
     CDS             6054..7166
                     /gene="mtnA"
                     /locus_tag="VGP50_06680"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEV8027096.1"
                     /translation="MKVQGKPTRTIWLDADGASVGIIDQTQLPHELVTKRLATLEDAA
                     HAISAMLVRGAPLIGATAAYGVALALGRNASDAALDAACRTLMATRPTAVNLRWAIEE
                     MRRELQPLPPGERAATAYRRAAVIADEDVETNRAIGAQGLPLIAAAWEEKQRSGPVEV
                     LTHCNAGWLATVDWGTALAPIYAAQEEGIPLHVWVDETRPRNQGASLTAWELGQQGVK
                     HTVIVDNAGGHLMQHGRVDLCITGSDRTTAAGDVANKIGTYLKALAAHDNGVKFYVAL
                     PSSTIDWSIEDGVRDIPIEERDPSEVAEIAGRLPDGKIARVRLVPEGSAALNYAFDVT
                     PARLVTGLITERGVCAASRAGLLSLFPERAARMAGE"
     gene            7318..9378
                     /locus_tag="VGP50_06685"
     CDS             7318..9378
                     /locus_tag="VGP50_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HEV8027097.1"
                     /translation="MKSRWSDSEAQAFVARYGEKGVGRDLALRVYTTRLLGSDPRLVL
                     HGGGNSSVKTTMRDLLGEEVEVLCVKGSGWDMGDIEPPGLPAVRLEPLRKLRRLERLS
                     DEDMVNAQRGNLLDSAAPNPSVETLLHAFLPHKFVDHTHSTAVLALTDQPEGEALCRE
                     VYDGRMGFVPYIMPGFALARKAAEIHERAPAVDGLILLKHGIFTFGASAEEAYERMIR
                     MVTLAEERLKQGRRNVFAAAALPAALAPVAEAAPILRGLCALANDRASGIAKRQVLCF
                     RSSPEIIDYVNGAEVTRYSQVGVVTPDHTIRTKNWPLVVPAPEAGRLDSWRACVAEAV
                     AIYAQRYHEYFARHNAEAAKPRTELDALPRVVLVPGLGLFGLGASAKDAAIAADIAEN
                     TVAVITDAEATGRYDCVGEADMFDVEYWSLEQAKLGKAAEKPLQRQVAVVTGGGSGIG
                     AATAKAFARAGAEVAVLDREGAAAQSVAKAIGAAARAIACDVTDAAAVRSGFDQVCAA
                     FGGVDVVVSNAGAAWQGSVGTLDDALLRRSFELNFFAHQHVAQNAVRVMRAQGTGGCL
                     LFNTSKQAVNPGRDFGPYGLPKAATLFLMKQYALDHGKDGIRSNAVNADRIRSGLLTD
                     DMIAQRSHARGVSAKDYMSGNLLGREVTAEDVAEAFVFLAQARKTTAAVLTVDGGNIE
                     ASLR"
     gene            9378..10229
                     /locus_tag="VGP50_06690"
     CDS             9378..10229
                     /locus_tag="VGP50_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV8027098.1"
                     /translation="MAGLVAGIDHVIIAVRDFGRVDLNWTRLGFALTPRGRHLQRGTA
                     NYCVMFATDYVELLGVVDPAQDLGGVDAFLAQREGIRSIAFASDAGSGTAAALAARGL
                     HPGTPRDLARQVELAEETIVPRFRLVALPPEETPGLDVLFCQHLTPELIRQPAWLAHP
                     NGALALHGVTVVVESTAPLAAAYEKLVGPAAVNKTDAVLTVHAGPHRIVFATPDDFSA
                     LYPEVTLASALTLPAAAVLSLVSRDIELTIDHLTQWQVPYETLADGAVLVPPEEANGA
                     ALLFTPV"
     gene            10295..>10508
                     /locus_tag="VGP50_06695"
     CDS             10295..>10508
                     /locus_tag="VGP50_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027099.1"
                     /translation="MTKRIVETSATDDMASEELVEDLDELNEVLPTNFSITSYGADYP
                     VDGLVKRITQGDIVVPVFSLEPEKGQS"
BASE COUNT         1679 a         3488 c         3598 g         1743 t
ORIGIN      
        1 ctgatcctgg tgcccttcgc gccgagcgcc gggatcgccg tggcgctgct gatggcgcgg
       61 agcctgctgt cgcagatgga cgtgccgacg cgcacgtcct atgtgatggc ggtggtgacg
      121 ccgggcgagc gcgccgccgc ggccagcgtc acctcggtgc cgcgcagcct cgccgcggcg
      181 gtgggcccga tcatcgccgg ctggctcctc gcgctctcgc ccttcggctg gtcgctggtg
      241 gccgcgggcg cgctcaagtc gatctatgat ctgctgctgc tcatgctgtt ccgcgggcac
      301 aagccgcctg aagagaccga gcgaacgcgc tgaaagcgcg ttcgtccgag gcgggcggca
      361 gcggttgctc ttgcaaccgc gagagcccgc acagagcaga acgcgcaccc cttccctctc
      421 ccgcaagggg cgagggggtt tccggtgtct acggccacgc gacttcgggc gggaggctca
      481 tcaggatcgc ttcgacgttg ccgccggtct tgaggccgaa gagggtgccg cggtcgtgga
      541 gcaggttgaa ctcgacatag cggccgcggc gcatgagctg atgcgcacgc tgctccggcg
      601 tccaggcttc ccacatatgg cggcggacga tcgccggata gatcgcgaga aaggccttgc
      661 cgatggcgcg gaggaacgtg aagtcgcgct cgggatcgcc gctgtcgaga tagtcgaaga
      721 agatgccgcc gacgccgcgc gactcgttcc ggtgcggcag gaagaaatac tcgtcgcacc
      781 atttcttgaa gcgcgggtaa taggccggat cgtgggcgtc gcaggcggcc tggagcgcgg
      841 cgtggaaggc ctgggtgtcg gccgcgtcgg gatagatcgg cgtcaggtcg ctgccgccgc
      901 cgaaccagcc cttggtggtg cggatgtggc gcgtgttcat atgtacggcc ggcaccagcg
      961 gcgagcgcat atgcgcgacc agcgagatgc ccgcggccca gaagcgcgga tcctcggcgg
     1021 cgccggggat ctgctggcgg aaatcggacg agaactcgcc ccagacggcg gagacgttga
     1081 cgccgacctt ctcgaagacg cggccgcgca gggtcgccat cgtgccgccg ccgccgccct
     1141 cgcgcgacca gggcttgcgc tcgaaacggc cgggcgcgcc ctcgtcgggg cggcgctgga
     1201 catactcggt ctcgagcgcc tcgaaagcag cgcagatgct gtcgcggagc tcattgaacc
     1261 accgcgccac ccggtcctga agcgcctcgg tcatcccctc tccttcggaa attgctcggt
     1321 ctgcctgagc gcctctccta gcaccatcgc cgccgccagc gccatgttga gggagcgcgt
     1381 ctcggggcgg agcggaatgc ggagccgcgc gtcggccgcc tgatgcaccg cctcgggcac
     1441 ccccgccgat tcgcggccga gaagcagcgt gtcgtcggcg cggaagcgga agcgatgata
     1501 ggcgaggtcg ccgcgcgtcg tcagcaggac gaggcgggtc tggttctggg cgaggaaagc
     1561 ggcccaggaa tcgtggcgcg cgagcgtggc gtggtcgagg tagtccatgc cggcgcggcg
     1621 gaagcgggcg tcgctcagaa cgaagccgca ggggccgatg atgtcgacgg cgacgccgag
     1681 acaggcgccg agacggagga gggcaccggc gttctggggg atgtccggct cgtaaagggc
     1741 gaggcgcata aggggaggaa agcgcgtagg gtggccggtt ggcaagtcgg ctgggagggg
     1801 gaagatgagt ctcgagggca aggacgcgtc atgatgtcgc atggctccga gcgcggtaat
     1861 gggtaggttc cgcgccgtca aatggcgccg cgggggcgag ttctcttgat caggagactc
     1921 gagggcgcag ccaaaggaga ggtggcgtga tggcggaagc ggcgcaggcg aggccgatgg
     1981 acccgcatac ccaggattcg ccgacgcggc gggacttcct caccatcgcc gccacggcgc
     2041 tcgccggcgt cggcgcggcg atcgcggtct ggccgctgat cgacagcatg aatgcggcga
     2101 aggacacgct ggcgctttcc accaccgagg tcgacctggc gccggtccag ctcggccagc
     2161 gcctcaccgt caaatggcgc ggcaatcccg tgttcatcga ccaccgcacg cccgacgaga
     2221 tcaaggcggc ggatgccgtc gacatcgcca ccttgcgcga tccccagacc gatggcgcgc
     2281 gcgtgcagaa ggcggaatgg ctgatcgtcg tcggcgtctg cacgcatctc ggctgcgtgc
     2341 cgctggggca gaaggcgacc gacaacagag gcgagtatgg cggctggttc tgtccctgcc
     2401 acggctcgca atacgacact tcggggcgca tccgccaggg gccggcgccg ctcaacctcg
     2461 cggtcccgaa atacgaattc accaccgaca ccagcgtcaa gatcggctag gagcgtcgct
     2521 cgatggctgc ccgtccgccg cagaacaaga tcataaggtg gctcgattac cggctgccga
     2581 tcttcggcat gctccatcac gagctctatg aatacccgac gcccaagaac ctgagctatt
     2641 ggtggaactt cgggtcgctg gccgggatca tgctgctgat catgatcatc accggcatct
     2701 tcctggcgat gcagtacacg ccgcatgtgg actatgcctt caattccgtc gagcgcatcc
     2761 ggcgcgacgt gaatttcggc tggctcatcc agtacatcca catgaatggc gggtccttct
     2821 tcttcatcgt cacctatatc catgtcttcc gcggcctcta ttacggctcc tacaaggcgc
     2881 cgcgcgagct tttgtggatg ctgggcgtcc tgatcctgct gttgatgatg gcgacggcct
     2941 tcatgggcta cgtgctgccc tggggacaga tgagcttctg gggcgccaag gtcatcacca
     3001 gcctgttctc ggcgatcccg gtggtcggcg attacatcgt ccactggctg tggggcggct
     3061 tcgcggtcga caacccgacg ctcaaccgct tctacgcgct ccactatctg ctgcccttca
     3121 tcatcgtcgc ggtggtgatg ctgcatctga tcgcgctgca cacgcacggc tccaacaatc
     3181 cgctcggcat cgacaccaag gggccgcagg acagcctccc cttccatccc tactacacct
     3241 tcaaggacat gttcgggctg gcggtgttcc tggtcgcgta ttgcatcttc atcttctaca
     3301 tgccgaacgc gctgggcgat ccgcagaact acatccccgc gaacccgctg cagacgccgc
     3361 ccgagatcgt gccggaatgg tatttcctgc cctattacgc gatcctgcgc tcggtgccga
     3421 acatcctgtt catcccggcg aagctcgccg gcgtgatgtg cctcttcggc gcgatcggtg
     3481 tgctcttcat cctgccctgg ctcgatacgt cgccggtgcg gagcgcgcgc ttccgtccca
     3541 tctacaagtg gctcttctgg gtgctgctga tcgatgtcgt ggtgctgggc atcgtcggcg
     3601 cgcaccggcc cgacctcatc gtgctgagcg tcaacgtgct ggggctcgac gtcgtcgtga
     3661 cctatctcgt catcggccag atcgcgacgc tctactactt cgtccatttc ctcctgctgg
     3721 tgccgctcat cggctggatc gagcagccgc tgccgctccc caccagcatc agcgtcgccg
     3781 tcgcgaagcc gggaggcggc cgcctggtcg gcgcctcgac gccgatggag aagccctgat
     3841 gcgccaagcc cttaccgtct tcggcggcgt catcatcgcg gtggtgctga tggccctgat
     3901 catcgccagc tacaggcatc cggcgccgac ctcggccggc gtgatcgagg agccgcagcc
     3961 ggtgctcccg caccagaaat ggtccttcga cggccccttc ggcacctatg accgcgcggc
     4021 ggtgcagcgc ggcttccagg tctacagcga aatctgctcg caatgccatt cgatgaccct
     4081 gatgcattac ggcgatctcg gtcccggcgg cccgggcggc ggcatcggct acaccgagga
     4141 cgaggtgaag gccatcgcgg cgacgccctt cgtcaatgcc aagcaggtga ccgacgggcc
     4201 caacgaccag ggccagatgt tccagcggcc ggggcgcccc tccgacaaat tcgtggcgcc
     4261 gttcgatcat cccgaggaag ccgccgccgc ttacggcgcg ataccgcccg atctctcggt
     4321 catcgtcaag gcgcgggccg gcggggccga ttatgtcttt ggcctcctca ccggctacaa
     4381 ggacgagccg ccgcccggcg tgaagatggc ggcgcccaat ctgcaataca acgaatactt
     4441 ccccggccac cagatcgcca tgccgccgcc gctcgaggac aaccgggtca cctatgcgga
     4501 cggcaccaag gcgacgctgg accaggaagc ccgcgacatc gccaccttcc tctcctgggc
     4561 gtccgagccg atcatggaag agaggaagcg cgacggcgcc aaggtgctga tcttcctcgc
     4621 cgtcatggtg atcgtgttct tcttcgccaa gcgcaaggtc tgggccgacg ttcactgaat
     4681 ttcccctctc ccgcttgcgg gagaggaagg gcccgttgcg cagcaacggg aaggtgaggg
     4741 ggcggctcag gcttgcccgc gcggtcccct caccctcccg tcgcgttgcg acgggtccct
     4801 ccctctcccg caagcgggag aggggattta tagtgacgcg gcatttcagc gtggagcgga
     4861 cccatggcga gtgacgcggt catcggcatc atcggcggca gcgggctcta tcagatcgac
     4921 gggctcaccg aggtcgaatg gcggcgggtg gcgtcgccgt tcggcgaggc ctcggacgag
     4981 ctctgcttcg gcaagctcgg cgggacgcgc gtcgtcttcc tgccgcgtca cggccgcggc
     5041 catcgcctgc cgccatcgga gatcaactac cgcgccaata tcgacgtgct gaagcgtgcg
     5101 ggcgccaccg acctgctgtc gctctcggcg gtggggagcc tcaaggcgga cctcgcgccc
     5161 ggcacattcc tgctggtcga ccagttcatc gaccgcacct tcgcgcggga gaagagcttc
     5221 ttcggcacag gcctcgtcgc ccatgtcgga ttcggccatc cggtgtgcgc gcggctcggc
     5281 gacgcgctcg cggcggccgg gaaagacgcg ggcctcgcga tgccgcgcgg cggcacttat
     5341 gtgtgcatgg aggggccgca attctcgacg ctggccgaaa gcaacctcca tcgcagctgg
     5401 ggctgcgcgg tggtcggcat gaccaacatg ccggaggcga agctcgcgcg cgaggccgag
     5461 ctctgctacg cctccgtcgc catggtcacc gatttcgact gctggcatcc cgaccatgac
     5521 cacgtcacgg tcgacatgat catcggcgtg ctcacggcga atgccgagcg cgccaagggg
     5581 ctggtgaagg cggtggtgcc gcttctcgcg cagcatcgcg gcgcctgccc ccatggctgc
     5641 cgcaacgccc tcgacaacgc gctcatcacg tcggttgagg cgcgcgaccc ggcgctggtg
     5701 gcaaagctcg acgcgatcgc gggacgcgcg ctgaagcgtt aggctcctag cgctggcccg
     5761 tccacaccat gaccatgagg cgacgatcct cgccgccggt ctggttgttg gagacgcggt
     5821 ccagcacgac gtcggtgagc ttcacctcga catcggcggc gagcgtctgg agccactcgt
     5881 tgatgtcgcc ctcgagcttc gccacctgct gattgccgct cacggtgaag atcttgacgc
     5941 tgttcatcgc gcgctccctc ggaagagggg ggtatttcgc gccaagctcc ttaacgccgg
     6001 cctaatggag tcgcctggaa gcggaacagc gcacgcgata tgatgcgcgg gcgatgaagg
     6061 tccagggaaa accgacgcgc acgatctggc tcgatgccga tggcgcgtcc gtgggcatca
     6121 tcgatcagac gcagcttccg catgagctgg tgacgaagcg gctcgccacg ctcgaggacg
     6181 cggcgcatgc gatttccgcc atgctcgtgc gtggcgcgcc gctcatcggc gcgacggcgg
     6241 cttacggcgt ggcactggcg ctcgggcgca acgcctccga cgcggcgctc gacgccgcgt
     6301 gccgcactct gatggcgacg cgcccgaccg cggtcaatct gcgctgggcg atcgaggaga
     6361 tgcggcgcga gctccagccg ctgcctccgg gcgagcgcgc cgccaccgcc tatcgccgcg
     6421 ccgcggtgat cgccgacgag gatgtcgaga ccaaccgcgc catcggcgcg caggggctgc
     6481 cgctcatcgc cgcggcctgg gaagagaagc agcgcagcgg gccggtcgag gtgctgacgc
     6541 attgcaatgc cggctggctc gctaccgtcg attgggggac ggcgctggcg ccgatctatg
     6601 cggcgcagga agaaggcatc ccgctccatg tctgggtcga cgagacgcgg ccgcggaacc
     6661 agggggcgag cctcaccgcc tgggagctcg gacagcaggg cgtgaagcac accgtcatcg
     6721 tcgacaatgc cggcggccat ctcatgcagc atggccgcgt cgatctctgc atcaccggct
     6781 ccgaccgcac caccgcggcg ggcgacgtcg ccaacaagat cgggacgtat ctcaaggcgc
     6841 tggccgcgca tgacaacggc gtgaagttct atgtcgcgct cccgtcctcg acgatcgact
     6901 ggtcgatcga agacggcgtg cgcgacatcc cgatcgagga gcgcgatccg tcggaggtcg
     6961 cggagatcgc cgggcggctg ccggacggca agatcgcgcg cgtgcggctt gtgcccgagg
     7021 gcagcgccgc gctcaactat gccttcgacg tgacgccggc gcggctcgtc accggcctca
     7081 tcaccgagcg cggcgtctgc gccgcctcgc gcgcgggtct gctctcgctc ttccccgagc
     7141 gcgccgcgcg catggcgggg gagtgagctt gagcgagcgg ggcgccgcta ccccccaccc
     7201 ctccttcccc ctcaaggggg gagggggaag atgtttgtgt tgcagcgaga ttttcccctc
     7261 cccccttgag ggggagggaa gggtgggggg tcgcttgcaa actcgcgatc gccgatcgtg
     7321 aaatcgcgct ggtcggacag cgaggcgcag gcctttgtcg cgcgctatgg cgagaagggc
     7381 gtcgggcgcg acctggcgct gcgggtttac acgacgcggc tcttggggag cgatccgcgg
     7441 ctcgtgctgc atggcggcgg caacagctcg gtcaagacga cgatgcgcga tctcctcggc
     7501 gaggaggtcg aggtgctctg cgtcaaggga tcgggctggg acatgggcga catcgagccg
     7561 ccgggactgc cggcggtgcg gctcgaaccg ctgcgcaaat tgcgccggct cgagcggctt
     7621 tcggacgagg acatggtgaa tgcgcagcgc ggcaacctgc tcgacagcgc cgcgcccaat
     7681 ccctcggtcg agacgctgct gcatgcgttc ctgccgcata aattcgtcga ccacacgcat
     7741 tcgacggcgg tgctggcact gaccgaccag cccgaaggcg aggcgctctg ccgcgaggtc
     7801 tatgacggac gcatgggctt cgtgccctat atcatgccgg gcttcgcgct cgcccggaaa
     7861 gcggcagaga tccatgagcg cgcgcccgcg gtcgacgggc tgatcctctt gaagcacggc
     7921 atcttcacct tcggcgcgag tgccgaggaa gcctatgagc gcatgatccg gatggtgacg
     7981 ctcgccgagg agcgcttgaa gcagggacgg cgcaacgtct tcgcggcggc ggcgctgccg
     8041 gcggcgctcg cgcccgtcgc cgaggcggcg ccgatcctgc gcggcctctg cgcgctcgcg
     8101 aacgaccgcg cgagcggcat cgccaagcgg caggtcctgt gcttccgcag ctcgcccgag
     8161 atcatcgact acgtcaacgg cgccgaagtg acgcgctatt cgcaagtggg cgtggtcacg
     8221 cccgatcaca ccatccgcac caagaactgg ccgctcgtcg tgccggcgcc ggaagccgga
     8281 cggctcgatt cgtggcgcgc ttgcgtggcg gaagcggtcg ccatctacgc gcagcgctat
     8341 cacgagtact tcgcgcgcca caatgcggaa gcggcgaagc cgcgcaccga gctcgatgcg
     8401 ctgccgcgcg tcgtgctggt gccggggctc ggcctcttcg ggctgggcgc gtccgcgaag
     8461 gacgcggcga tcgccgccga catcgccgag aacacggtcg cggtcatcac cgatgccgaa
     8521 gcgacgggac gctatgactg cgtcggcgag gccgacatgt tcgacgtcga atactggtcg
     8581 ctcgagcagg cgaagctcgg caaggcggcg gagaagccgc tgcagcgcca ggtcgcggtg
     8641 gtgacgggcg gcggcagcgg catcggcgcg gcgacggcga aagctttcgc ccgcgccggc
     8701 gcggaagtcg cggttctgga ccgagagggc gcggcagcgc agtcggtcgc aaaagcgatc
     8761 ggcgccgctg cgcgcgcgat cgcctgcgac gtcaccgacg cggcggcggt gcgctcaggc
     8821 ttcgatcagg tgtgcgcggc gttcggcggc gtcgatgtcg tcgtctccaa tgcgggggcg
     8881 gcgtggcaag gcagtgtcgg cacgctcgac gacgcgctgc tgcgccgctc tttcgagctc
     8941 aacttcttcg cgcatcagca cgtggcgcag aacgcggtgc gggtcatgcg cgcccaggga
     9001 accggcggct gcctgctctt caatacctcg aagcaggcgg tcaatcccgg ccgcgatttc
     9061 ggcccgtacg ggttgcccaa ggcggcgacg ctgttcctga tgaagcaata cgcgctcgat
     9121 cacggcaagg acggcatccg ttccaacgcc gtcaacgccg accgcatccg tagcggcttg
     9181 ctcacggacg acatgatcgc gcagcgctcg cacgcccgcg gcgtctcggc caaggactat
     9241 atgagcggca atctgctggg acgcgaggtg acggccgagg acgtcgccga ggccttcgtc
     9301 tttctggcgc aagcgcgcaa gaccacggct gcggtgctga ccgtcgatgg cggcaacatc
     9361 gaagccagtt tacgctgatg gccgggctcg tcgccggcat cgaccacgtc atcatcgccg
     9421 tccgcgactt cggccgtgtc gacttgaact ggacgcggct cggcttcgcg ctgacgccgc
     9481 gcggccgcca tctgcagcgc ggcacagcca attactgcgt catgttcgcg acggattatg
     9541 tcgagctgct cggcgtcgtc gatccggcac aggatctggg cggggtcgac gctttcctgg
     9601 cgcagcgcga agggattcgc agcatcgcct ttgccagcga tgccgggagc gggacggcgg
     9661 cggcgctcgc cgcgcgcggc cttcaccccg gcactccccg tgatctggcg cggcaagtgg
     9721 agctggcgga ggagacgatc gtgccgcgct tccgcctcgt cgcgctcccg cccgaagaga
     9781 cgccgggcct cgatgtgctt ttctgccagc acctgacgcc ggaactcatc cgccagccgg
     9841 cttggctcgc gcacccgaat ggcgcgctgg cgctgcacgg cgtgacggtg gtcgtggagt
     9901 cgacggcgcc gcttgctgcg gcctatgaga agcttgtggg tcccgcggcg gttaacaaga
     9961 ccgacgcggt gctgaccgtc catgccggtc cacaccgtat cgtcttcgcg acgcccgacg
    10021 atttttccgc gctctatccc gaggtgacgc tggcgtctgc gctgaccctt ccggccgccg
    10081 ccgtgttgtc gctggtgagc cgcgatatcg agctcacgat agaccacttg acgcaatggc
    10141 aggtgcccta tgagacgctg gccgacggcg ccgtcctggt gccgccggaa gaagcgaacg
    10201 gcgcggcgct gcttttcacc ccggtttaag gctgtcatag ttgctatcag gttgaccgtg
    10261 caaccatgtc gactaattgc caagcgagat ctatatgacc aaaagaatcg tggaaacgtc
    10321 agctactgat gacatggcgt ctgaagaact cgtcgaggat cttgacgagc tgaacgaggt
    10381 actcccaacg aatttttcga tcacttccta cggcgctgac tacccagtcg atggattggt
    10441 caagcgaata acgcagggag acatagtcgt gccggtcttt agcctcgagc ctgagaaggg
    10501 acaatcta
//
LOCUS       DASYNS010000216         6820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4571969, whole genome shotgun sequence.
ACCESSION   DASYNS010000216 DASYNS010000000
VERSION     DASYNS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4571969"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..351
                     /locus_tag="VGP50_06700"
     CDS             1..351
                     /locus_tag="VGP50_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010516231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV8027100.1"
                     /translation="MDLAASKKFYDAVLGAIGLPAGQADPKGRIFYRTKTGVFGITRP
                     IDGKAATHANGGTIGFGCASPEEARAFHDAGVANGGKSIEDPPGWREGPAGKFYLAYL
                     RDPSGNKICALHRG"
     gene            complement(491..775)
                     /locus_tag="VGP50_06705"
     CDS             complement(491..775)
                     /locus_tag="VGP50_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV8027101.1"
                     /translation="MYLSNESLFVILVVGLVAGWLAGHIMRGTGFGLIGDIVVGIIGA
                     FIGDWLLPQLHIHLGVGIVSAIINATIGGIVLLLIVSLVRGSGRRSWGRR"
     gene            complement(1050..2069)
                     /locus_tag="VGP50_06710"
     CDS             complement(1050..2069)
                     /locus_tag="VGP50_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV8027102.1"
                     /translation="MALVASTRGEETKKPATLDRLSQLLQSDIDHVNTLILERMQSPV
                     ALIPQLAGHIIAAGGKRLRPMLTLAASRMCGYRGDRHVALAAAVEFIHTATLLHDDVV
                     DASDLRRGLATANAVWGNKSSVLVGDFLFSRAFQLMVEDGSLRVLAILSEASAIIAEG
                     EVLQLVTSNDTETTEAAYLAVIEAKTAQLFAAASRLGAVITERPYEEEDALAAYGRNL
                     GVAFQLVDDMLDYSARQAELGKTVGDDFREGKITLPVVLALRQAGDVERAFWRRTLEE
                     LDQQEGDLAHAIELMTRHGTLRATLDSARRYAETARRALSLFRDGPEKRALDEAIDFC
                     LARGH"
     gene            2187..2396
                     /locus_tag="VGP50_06715"
     CDS             2187..2396
                     /locus_tag="VGP50_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEV8027103.1"
                     /translation="MKELLRTNDAVRLSWLQALLAGAGIESVILDTNTSIIEGSIGAI
                     QRRLMVTDDDFRRASTLLAEAGEER"
     gene            2393..3148
                     /locus_tag="VGP50_06720"
     CDS             2393..3148
                     /locus_tag="VGP50_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEV8027104.1"
                     /translation="MSEAARDLPLSEDALLGGRIRLRQPVHGYRVAIDPVLLAAAVPA
                     GAGDSVLDIGCGVGAASLCLAARVADCRITGLEMERALVRLANDNAALNGCADRVAAI
                     VGDLLHAPPLLALASFAHVMANPPFLERDAASLPRHPGKAAATMEGAADLAAWLDFAL
                     AMLLPKGSLTIIHRADRLEHVLAGLAGRSGEIVVFPLWAGAGKAAKRVLIRARKGVAT
                     PTRLAPGLALHEADGRFTAAAEAILRGGAALLL"
     gene            3192..4049
                     /locus_tag="VGP50_06725"
     CDS             3192..4049
                     /locus_tag="VGP50_06725"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEV8027105.1"
                     /translation="MSIVDELLQKLPLERFRNPPPQVAVLRLDGLIGMRGPRGLSLRR
                     FAAAIERAFALRRLKAVALVVNSPGGSPAQSSLLFRRIRQLAEEHEVPVVAFAEDVAA
                     SGGYWLALAADEVFADETSLLGSIGVITASFGFTEALRKLGVQRRLYTAGDNKSMLDP
                     FLTEDPKAVERLTVLQRDMHEAFKDLVRSRRGARLKGEESVLFSGEVFTGRRALELGL
                     IDGIGDLRGVMRQRFGDTVRLVGIEPERRRFALLSRFGFPRRPDIADIAADLLTRVEE
                     RLIWARFGL"
     gene            4062..4322
                     /locus_tag="VGP50_06730"
     CDS             4062..4322
                     /locus_tag="VGP50_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027106.1"
                     /translation="MLGLGLPKLILLALMIAIIWYGYRYLQRIEAVRRALRDELMRRQ
                     RATARPSQPVEAEDLVKCATCSAYVPSHHASACGRPDCPWRR"
     gene            complement(4486..5598)
                     /locus_tag="VGP50_06735"
     CDS             complement(4486..5598)
                     /locus_tag="VGP50_06735"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV8027107.1"
                     /translation="MTVMDDQAVAERVLAHIANGTTDVGAEVWREPVVNYRSEERLAA
                     EIAQVFRRSPTPFCPSAALPDPGSYVARAAAGVPIVAVRGADGTVRAFRNACRHRGMA
                     LADGSGCARAFVCRYHGWTYNLEGRLRHIPHAGGFPGFDKEAHALVPVAAQERLGLVF
                     VTQDAPPPEDHALDALAKLVAPDQFLFATTERDFDVNWKLLIEGLIEGYHIRSTHPKS
                     FLPYGFDNLNVVELFGRHSRVTYPFKRIEKLASLPAQERRIEGRWSYVYHLFPNVALT
                     VHSRHTALLILEPLSSGRTRQVIYWLTSGGGDDPEAIAEAKRDVEFVMAGAAEDRAVV
                     EAIQHGLASDANDAFTFGRFESAIVHFHRTLTQALG"
     gene            5778..6362
                     /locus_tag="VGP50_06740"
     CDS             5778..6362
                     /locus_tag="VGP50_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027108.1"
                     /translation="MHAASLRTTFLTAGLCLALAGCSIPDRDGGASDASPDPALQQGS
                     SPSAPAVDQATVRQASAEAWPGRNVGSPISLGVTPAQSGDAAVAERGGAAGIERVSLP
                     APPGSDGLSGSSTGPSGLVDVNLNKVRRAVARYLNVDARKIPATIVLPVTVAASLCGI
                     DATALTKQQRPGGKPICMAKNAGMASDVVEPTIQ"
     gene            6550..>6820
                     /locus_tag="VGP50_06745"
     CDS             6550..>6820
                     /locus_tag="VGP50_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8027109.1"
                     /translation="MPRIAATALPRRRLDQRFDTLRLLVAEATPPAAGWIRAIRKSLG
                     MTVAQLAVRLGVRRETARDIETSEVRGSITLDTLRRAAQALDCALV"
BASE COUNT         1067 a         2333 c         2307 g         1113 t
ORIGIN      
        1 atcgatctcg ccgcgtccaa gaagttttac gacgcagtgt tgggcgccat cggccttcct
       61 gcgggccaag ccgatccgaa gggccgcatc ttttatcgca ccaagacggg cgtgttcggc
      121 ataaccaggc cgatcgatgg caaggcggcg acgcacgcca atggcggcac gatcggcttc
      181 gggtgcgcca gccccgagga ggccagagcc ttccatgacg ccggtgtcgc caacggtggc
      241 aagagcatcg aggacccgcc cggctggcgc gaagggcccg cgggcaagtt ctatcttgcc
      301 tatctgcgcg atccatccgg caacaagatc tgtgcgctac atcgcggctg aacgcgcatc
      361 gttgccgtca tctgctgagc tcagccattc ggcgaccgcg cgaccgccgc ttggggtcaa
      421 ctgctgctgt gggctcacgc gccatggcgc ggcggatctt ccttgaggag cggaaggacc
      481 ccatcatctc ctagcggcgc ccccagctcc ggcggccgct gccgcggacg aggctgacga
      541 tgagcagcag cacgatgccg ccgatggtcg cgttgatgat cgcgctgaca attccgacac
      601 cgagatggat gtggagttgc ggcagcagcc agtcgccgat gaaggcgccg atgattccga
      661 cgacaatgtc gccaatcaat ccaaagcccg tcccgcgcat gatgtggccg gccaaccatc
      721 cggcgacaag accgacgacg aggatgacga agaggctttc gttggacagg tacatggcaa
      781 tttcctccgg ggcgagaccg tcatctcaat cgagcggcca gcacaccgca aatcgccgga
      841 cgccaaccgt ggtgcgcgga caagacatca ccggcagaat cgtgcactct ctgccgccga
      901 gcctccgcca gggcaagcgc gtcatcggcc tttccctgcc gtctcgaagg gtaacccgag
      961 cgcagccgca atgttccgac ggcgcgtgaa tttttctttc tccgcaacgc ggtgatttcg
     1021 ttgtgtggct tgctatgcct tgccgatgct caatgcccgc gcgccaggca gaagtcgatg
     1081 gcttcgtcga gggcgcgttt ttcggggccg tcgcggaaga gagagagggc gcggcgcgcc
     1141 gtctcggcgt agcggcgcgc ggaatcgagc gtcgcgcgca gcgtgccgtg ccgcgtcatc
     1201 agctcgatgg catgggcgag atcgccttcc tgctggtcga gctcctcgag cgtgcgccgc
     1261 cagaaggcgc gctcgacatc gcccgcctgg cgcagcgcca gcaccaccgg cagcgtgatc
     1321 ttgccctcgc ggaaatcgtc gcccacggtc ttgccgagct cggcctggcg cgccgaatag
     1381 tcgagcatgt cgtcgacgag ctggaaggcg acgccgagat tgcgcccata ggccgcgagc
     1441 gcgtcctctt cctcgtacgg ccgctcggtg atgacggcgc cgagacggct cgcggcggcg
     1501 aagagctgcg ccgtcttcgc ttcgatgacg gcgagatagg cggcttccgt cgtctcggtg
     1561 tcgttcgagg tgacgagctg cagcacctcg ccttcggcga tgatggccga tgcctcggag
     1621 aggatggcga gcacgcgcag cgacccgtcc tcgaccatga gctggaaagc gcggctgaag
     1681 agaaaatcgc cgaccagcac cgaggatttg ttgccccaga cggcgttggc cgtggccagg
     1741 ccgcggcgca aatcgctggc gtcgacgacg tcgtcatgga gcagcgtcgc ggtgtggatg
     1801 aactcgacgg cggccgcgag cgccacgtga cgatcgccgc gatagccgca catgcgcgaa
     1861 gccgccagcg tcagcatggg gcggaggcgc ttgccgcccg cggcgatgat gtggcccgcg
     1921 agctgcggaa tgagcgcgac cgggctctgc atccgctcga gaatgagggt attgacgtgg
     1981 tcgatgtcgg attggaggag ctggctcaac cggtcgagcg tcgccggttt cttggtctcc
     2041 tcgcctcgcg tgcttgccac cagggccacc gcactatccc tttccccacc gcgagcccgc
     2101 cgcgctcgct tgacggcgcg ccggccgcac gcgagcatag agggcctgcc aaaggggtca
     2161 agccgggcac tgcgctcggt ccatccatga aagagctgct tcgcaccaat gacgccgtcc
     2221 gtctgtcctg gctccaggca ttgcttgccg gcgccggcat cgagagcgtc attctcgaca
     2281 ccaacaccag catcatcgaa ggcagcatcg gcgccattca gcggcgcctc atggtgaccg
     2341 atgacgactt ccgccgcgcc agcacgttgc tggccgaagc gggagaggag cggtgagcga
     2401 agccgctcgc gacctgccat tgagcgaaga tgcgttgctg ggcggccgga tcaggctgcg
     2461 ccagccggtg cacggctatc gcgtggccat cgatcccgtg ttgcttgccg ccgccgtccc
     2521 ggctggagcc ggcgacagcg tgctcgacat cggctgcggc gtcggcgccg cgtcgctctg
     2581 tcttgcggcg cgtgtcgcgg attgccggat caccggtctc gagatggagc gggcgctggt
     2641 gcgccttgcc aacgacaatg cggcgctcaa tggctgcgcc gaccgcgtcg cggcgatcgt
     2701 tggcgatctg ctccacgccc cgccacttct ggcgctggcc agctttgcgc atgtcatggc
     2761 caatccgccc ttcctcgagc gcgacgcggc gagtctgccg cgccatccag gaaaggccgc
     2821 ggccactatg gagggcgcgg ccgatctcgc cgcctggctc gatttcgcgc tcgccatgct
     2881 gcttccgaag ggcagcctca ccatcatcca ccgcgccgat cgcctcgagc atgtgctggc
     2941 ggggctcgcg ggacgcagcg gcgagatcgt cgtgttcccg ctctgggccg gcgccggcaa
     3001 ggcggcgaag cgcgtgctca tccgcgcgcg caagggcgtc gcgacgccga cccgcctcgc
     3061 ccccggcctg gcgctgcacg aggcggacgg gcgcttcacg gcggcggccg aggccatcct
     3121 gcgcggcggt gcggcgctcc tcctgtgagc accttgccaa ggcccgggca cgatgcccat
     3181 cttgttgctc catgagcatt gtcgacgagc ttctccagaa gctgccgctg gagcgtttcc
     3241 gcaatccgcc cccgcaagtc gcggtgttgc ggctcgacgg cctcatcggc atgcgcggcc
     3301 cgcgcggcct atcgctgagg cgctttgccg ccgccatcga gcgcgccttc gcgctgcgcc
     3361 gcttgaaagc ggtggcgctc gtcgtcaatt cgcccggcgg ctcgccggcg caatcctcgc
     3421 tgctcttccg ccgcatccgc cagctcgccg aagagcacga ggtgccggtg gtcgctttcg
     3481 ccgaggatgt cgcggcctcg ggcggctatt ggctggcgct cgcggccgac gaggtctttg
     3541 ccgacgagac gtcgcttctg ggctcgatcg gcgtcatcac cgccagcttc ggtttcaccg
     3601 aggcgctgcg caagctcggc gtgcagcgtc gcctctatac ggccggcgac aacaaatcca
     3661 tgctcgatcc cttcctcacc gaggatccga aggcggtcga gcggctgacg gtgctgcagc
     3721 gcgacatgca tgaggccttc aaggacctgg tgcgcagccg ccgcggcgcg cgcctcaagg
     3781 gcgaggagag cgtgctcttc agcggcgagg tcttcaccgg ccggcgcgcg ctcgagctcg
     3841 gcctcatcga cggcattggc gacctgcgcg gcgtgatgcg ccagcgcttt ggcgacactg
     3901 tgcgcctcgt cggcatcgag cccgagcggc ggcgcttcgc cttgctgtcg cgcttcggct
     3961 tcccgcgccg tcccgacatc gccgacatcg ccgccgatct cctgacgcgc gtcgaggagc
     4021 ggctgatctg ggcccgcttc ggcctttaga tgcgctagcg catgctcggc ctcggcctcc
     4081 ccaagctcat cctcctcgcc ctcatgatcg cgatcatttg gtacggctac cggtacctgc
     4141 agcgcatcga agcggtgcgg cgcgcgctgc gcgacgagct gatgcggcgg cagcgagcga
     4201 cggcgcggcc atcgcagccg gtcgaggccg aagatctcgt caaatgcgcg acttgcagcg
     4261 cttatgtgcc gtcgcaccac gccagcgcct gcggccgccc ggactgtccc tggcggcgct
     4321 gacggtgctt cagagcagac ccccagcaag cggaatcgac gcttcctaat ctcgtcattc
     4381 ccgcgaaagc gggaacccag gggctgccgg cacgtcgctc gccctgggtc cccgctttcg
     4441 cggggatgac ggtgaaggaa aggttccgcg tatacgggat gcgctctaac cgagcgcctg
     4501 cgtcagcgtg cggtggaaat gtacgatggc gctttcgaag cggccgaagg tgaaggcgtc
     4561 gttggcgtcg ctggcgaggc cgtgctggat ggcttccacc acggcgcggt cttccgcggc
     4621 gccggccatg acgaattcga cgtcgcgttt tgcctcggcg atcgcctcag gatcgtcgcc
     4681 gccgccgctg gtcagccaat agatcacctg ccgtgtccgg ccggacgaca atggctccaa
     4741 gatcaggagc gcggtgtggc gcgagtggac cgtgagcgcc acgttcggga agagatggta
     4801 gacataggac cagcggccct cgatccgccg ttcctgcgcg ggcaggctcg cgagcttctc
     4861 gatgcgcttg aacggatagg tgacgcggct gtggcggccg aacagctcga cgacgttgag
     4921 gttgtcgaaa ccgtagggca ggaagctctt cggatgcgtc gagcggatgt gatagccctc
     4981 gatcaagcct tcgatcagga gcttccagtt gacgtcgaaa tcgcgctccg tggtggcgaa
     5041 gagaaactgg tccggcgcga ccaacttggc gagcgcgtcg agcgcgtggt cctcgggcgg
     5101 cggcgcgtcc tgcgtcacga agacgaggcc aaggcgctcc tgcgccgcga cgggcacgag
     5161 ggcatgcgcc tctttgtcga agccgggaaa tccgcccgca tgcgggatgt ggcgcaggcg
     5221 tccttcgaga ttgtaggtcc agccgtggta gcggcagaca aaggccctgg cgcagcctga
     5281 gccgtcggca agcgccatgc cgcgatgccg gcaggcgttg cggaacgcgc ggaccgtccc
     5341 atcggcgccg cgcacggcga cgatgggaac gccggccgcc gcgcgcgcca cataggagcc
     5401 cgggtcgggc agcgccgccg acgggcagaa cggcgtcggc gagcggcgga agacctgcgc
     5461 gatctcggcg gcgaggcgct cttccgagcg ataattgacg accggctcgc gccacacctc
     5521 cgcgccgaca tcggtcgtgc cgttggcgat gtgcgcgagg acgcgctctg ccaccgcctg
     5581 gtcgtccatt accgtcatct ttgccgcctc tccttgcttg ccgccccgag catacgccgc
     5641 ctccggaacg cgatgaagcg gcgagcgttg atccgcgata gagaccccgc aaccgaacgg
     5701 cgtgacgcgg ccccgcctga tccccggcgt ggcggcgcgc cgtcgcagac tcgggccgtt
     5761 gcggaggagg tggcgacatg cacgcagcga gcctccggac cacatttctg acggcgggcc
     5821 tgtgcctcgc tctcgcgggt tgctcgatcc cggatcggga cggcggcgcg tcggacgcct
     5881 cgcccgaccc cgccctccag caaggcagca gcccgtccgc gccagcggtc gatcaagcga
     5941 cggtgcggca ggccagtgcc gaggcctggc ctggccgcaa cgtcggctcc ccgatctcac
     6001 tcggcgtcac gccggcacaa agcggcgacg ccgcggtcgc cgagcgcggc ggcgcggccg
     6061 gcatcgagcg cgtgtccctg ccggcgccgc cgggcagcga cggcctctcc ggcagcagca
     6121 ccggtccgtc cggtctcgtc gacgtcaatc tcaacaaagt acgtcgcgcc gtcgcccgct
     6181 acctcaacgt cgacgcgcgc aagataccgg cgacgatcgt gctgccggtc accgtggcgg
     6241 ccagcctctg cggcatcgat gccacggcgc tgaccaagca gcagcggccg ggcggaaagc
     6301 cgatctgcat ggcgaagaac gccggcatgg cgagcgacgt cgtcgagccg acgatccagt
     6361 aggttcgacg cgcaggggtt ctggcgtatt ccatcgaaat gcgccgatac ttgccgcctt
     6421 atataaagtt atatggctga tatagacgcc tattaatcga aaaataggtg cctatatacg
     6481 ccattaatgt tgacatctgg cgcccgccgg ccatagtgcg cgccgaccca atgctacagg
     6541 gaacgcatca tgccgcggat cgctgccacc gctctccccc gccgtcgcct cgaccagaga
     6601 ttcgacacct tacgactatt ggttgccgag gcgacgcctc ccgccgccgg gtggatacgc
     6661 gccatccgca aaagcctggg catgaccgtg gcccaacttg ctgtccggct tggcgttcgg
     6721 cgggagaccg cccgcgatat cgagacgtcc gaggtccggg gatcgatcac cctcgacacg
     6781 cttcggcggg ccgcacaggc gcttgactgc gcgttggtgt
//
LOCUS       DASYNS010000217         4655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2232497, whole genome shotgun sequence.
ACCESSION   DASYNS010000217 DASYNS010000000
VERSION     DASYNS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2232497"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VGP50_06750"
     CDS             complement(<1..407)
                     /locus_tag="VGP50_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV8027110.1"
                     /translation="MEIADLLTPAAVIPNLRATNKRQALQELAKRAAALTGRQERMIF
                     EVLLERERLATTGIGSGTAIPHGRLADLPRLYGLFARLEKPIPFEAIDDQPVDLIFLL
                     LTPDNAGADHLKALARISRLLRDKAICDKLRGT"
     gene            complement(407..1015)
                     /gene="raiA"
                     /locus_tag="VGP50_06755"
     CDS             complement(407..1015)
                     /gene="raiA"
                     /locus_tag="VGP50_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV8027111.1"
                     /translation="MQVIVTGKQIDVGEALRGHAESATAAIVERYFGKAIEAHVVFSR
                     ERHLILSNISVHARRGLLVQCHGESSDPYAAFDSASERLDKQLRRYKRRLRNHHRNAK
                     EESEPEDATDYVVAAAGEEEQGAEQADDQPLVIAEMRTSIPRLSVSEAVMRLDLTDLP
                     ALLFRNSAHGNINLVYRRRDGNVGWIDPDLASQSRRAANKGS"
     gene            complement(1118..2470)
                     /locus_tag="VGP50_06760"
     CDS             complement(1118..2470)
                     /locus_tag="VGP50_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027112.1"
                     /translation="MLKSTTVLGIIAPLAALFVFFLLSALYAFGARDVYAAVMSHWGI
                     EPFPFDITFGDMQGLLAAWQCDRLGTDVVVSDPCDILHRPFNYSPLWLVASPLGLGIG
                     ATKAAGWLSGLAFLLSLFLLPPPRRARELPFVILATLSTMVAFAVERGNPDLMIFMLA
                     LAMGYLALRSSRWRFFAYGVGLVAGLLKYYPLTLLALTVRERVSTFVVVNVVALALIV
                     AFAAGYHAEIERGLPTIASGIYYGDMFAAKNLPFGIAETVLGSGDATAADASPPHLLG
                     LVLEALLLLFCAANSARLIRGSGLRQGLATLTPRESIFLAIGGVLIIGCFFAGQNVGY
                     RGIFLLLVLPGLLAVARNAPDKATQGLLKFASVLIVLLMWGEFFRTNLIFALRGFHFG
                     EGAVLAGWLGFWLLRELAWWWAISVLAATTLWLLLQSPVGRRASSALPRRVPAGGPAT
                     "
     gene            complement(2488..2886)
                     /locus_tag="VGP50_06765"
     CDS             complement(2488..2886)
                     /locus_tag="VGP50_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027113.1"
                     /translation="MYYTISFALMIGFTVAANVFLKAGAVVPAAERTIPGVCDWRTLI
                     GFAFFGTSAIIYSGVLQSMPLNMAQSIAASQFVAVILASAVILAEPIPPLRLLGILLI
                     VAGVAVVSVSGKRSDASAAIAASHAEETPH"
     gene            complement(2891..3856)
                     /locus_tag="VGP50_06770"
     CDS             complement(2891..3856)
                     /locus_tag="VGP50_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8027114.1"
                     /translation="MIAAPTIAVRGAALSDILPLLRCPETGAQLVQRGDRLQSASGDR
                     VYAVTESGIPLFAERFCSDHARIQEAHYEKIAAAYIENLAYPHTQEYMASLDRALFAA
                     IPGERLGTVAELCCGHAEAFQLFDARIGRGIGVDISVSMLEAARRDNTTRHLSFVQGD
                     ATRLPLASDAFDTVFMLGGIHHVSDRRALFAEVARILKPGGWFYFREPVSDFALWRWI
                     RALVYRLSPMLDHETERPLLYRETAPVLAASGLSLRHWTTHGFVGFCLFMNSDVLVFN
                     RLFRFMPGIRAMARLAAGIDAACLKLPFLRHAGLQVVGAACKEPG"
     gene            complement(3853..>4655)
                     /locus_tag="VGP50_06775"
     CDS             complement(3853..>4655)
                     /locus_tag="VGP50_06775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8027115.1"
                     /translation="VAAGSPERFGYEWHTYAEMRAEYEEQFRRWTVHLSPEDWRGKLF
                     LDVGCGMGRNSYWPMTYGAAGGVAIDVDERSLASARRTLSSCPKVEVRACSAYAIGDI
                     ERFDIVFSIGVIHHLEAPEKALAAMVTAARRGGKVLIWVYGRENNGLVVDVLTPLRKL
                     LFSRLPIGMVHHLSLYPSVALWLALRLGLDSNEYFRLLRRLQFRHLRSIVFDQMLPKI
                     AHYWPRATVEALMRASGLVDIRLSWVNQVSWSAIGTKPAAAASGRPQP"
BASE COUNT          919 a         1467 c         1604 g          665 t
ORIGIN      
        1 tcggtcccgc gcagcttgtc gcaaatggcc ttgtcgcgga gcaggcggga aatccgcgcc
       61 agcgccttca gatggtcggc accggcgttg tcgggggtca gcaacaggaa gatcagatcg
      121 acgggctggt catcgatcgc ctcgaaaggg atcggctttt ccaagcgggc gaacaggccg
      181 tagagccgcg gcagatcggc gaggcgtcca tgcgggatgg cggtgccact gccgatgccc
      241 gtcgtggcca ggcgctcgcg ctcgagcagc acctcgaaaa tcatgcgctc ctgccgcccg
      301 gtcagcgccg ccgctcgctt ggcgagctcc tgcaatgctt ggcgcttgtt cgtggcgcgc
      361 aggttcggaa tcacggcggc cggcgtcaac agatcggcga tttccatcag gagcctttgt
      421 tggcggcgcg gcggctctgg ctggccagat cggggtcgat ccagccgaca ttgccgtcgc
      481 gccgtcgata gacgaggttg atattgccat gggcgctgtt gcggaagagc agtgccggca
      541 gatcggtcag gtcgagccgc atcaccgctt cgctgacgct gagccgcggg atgctggtgc
      601 gcatctcggc gatgaccagc ggctggtcgt cggcctgttc cgcgccctgc tcctcttcgc
      661 cggctgcggc caccacataa tcggtcgcat cctcgggctc gctctcctcc ttggcgttgc
      721 gatgatgatt gcgcaggcga cgcttgtagc ggcgcaactg cttgtcgagc cgctccgagg
      781 cgctgtcgaa ggcggcataa ggatcgctcg actcaccatg gcactgcacc aggaggccgc
      841 ggcgcgcatg caccgatatg ttcgacagta tcaagtgacg ctcgcgggag aacaccacat
      901 gagcctcgat cgccttgccg aaataacgct caacgattgc cgccgtcgca ctctcggcat
      961 ggccgcgtaa ggcttcgccc acatcgatct gcttgcccgt gacgattact tgcatgaggc
     1021 attaccggat cggccaggcc caatcttctt taacacaccc gagccaatct ttatcccacc
     1081 ggggcggccc ccttatcgcc ccggggggtg ggaggtgtca agtagcgggc ccgcccgccg
     1141 gaacacggcg cgggagagcg gaagacgccc gtcgccctac cggggattgc aacaaaagcc
     1201 agagcgtcgt cgccgcgagg acgctgatcg cccaccacca tgccagctcg cgcagcagcc
     1261 agaagccgag ccagccggca agcacggcgc cttcgccgaa atggaacccg cgcaaggcga
     1321 agatcaggtt ggtgcggaag aactcgcccc acatcaggag cacgatcagg acgctcgcga
     1381 atttgagcaa accctgcgtc gccttgtccg gcgcgttgcg cgcgacggcg agcaggcctg
     1441 gaagcaccag caggagaaat atgccgcgat agccgacatt ctgcccggcg aaaaagcagc
     1501 cgatgatgag aacgccgccg atggcgagga agatcgattc gcgcggcgtg agcgttgcca
     1561 ggccctggcg aaggccggat cccctgatca ggcgcgcgct gttggcggcg cagaagagca
     1621 gcaggagcgc ctcgagcacg agaccgagga ggtgcggcgg cgaggcgtcc gccgccgtgg
     1681 cgtctccgct gcccagcact gtctcggcga tgccgaaggg cagattcttg gcggcgaaca
     1741 tgtcgccgta atagatgccg gaggcgatgg tcgggaggcc tcgctcgatt tcggcgtgat
     1801 aacccgccgc aaaggcgacg atgagggcga gcgcgacgac gttcacgacg acgaaggtcg
     1861 agacccgttc ccgcacggtg agcgcgagaa gggtcaacgg gtagtatttg aggagcccag
     1921 cgacgagccc gacgccatag gcgaaaaagc gccagcggga ggagcggagg gcgaggtatc
     1981 ccatggcgag cgcgagcatg aaaatcatca ggtcggggtt gccgcgttcg acggcgaagg
     2041 cgaccatcgt cgagagcgtc gccagaatga cgaatggcag ctcccgcgcg cgccgggggg
     2101 gcggcagcag gaacagcgag agcaggaaag cgaggccaga gagccagccc gcggccttgg
     2161 tggcaccgat ccccagcccg agcggcgagg cgacgagcca gagcggcgaa tagttgaagg
     2221 gccggtggag gatgtcgcag gggtcggaga ccacgacatc ggtgccgagg cgatcgcact
     2281 gccacgccgc cagcaggccc tgcatatcgc cgaaagtgat gtcgaagggg aagggttcga
     2341 tgccccaatg gctcataacg gccgcgtaga cgtcccgcgc accgaacgca taaagcgcgg
     2401 agagcaaaaa gaagacgaaa agcgcggcca gcggcgcgat gatcccgagc accgtcgtcg
     2461 atttcaacaa ggagaaacgt ccggcggtca gtgaggcgtc tcctctgcat gggaagcggc
     2521 aatggccgcg gaagcgtcgg agcgttttcc cgagacggag acgacggcga cgccggcgac
     2581 gatcagaagg attccgagaa ggcgcagcgg cggtatcggc tctgccagaa tcaccgccga
     2641 cgccaggatc accgcgacga actgggaggc ggcgatcgac tgcgccatgt tcagcggcat
     2701 cgattgcagc acgccggaat agatgatcgc cgaggtgccg aagaaagcga agccgatgag
     2761 cgtccgccag tcgcagacgc ccgggatcgt gcgctcggcc gccggcacaa ccgcgcccgc
     2821 cttcagaaag acattggcgg ccacggtgaa gccgatcatc agcgcaaagg agatcgtgta
     2881 atacatgagg tcacccaggc tccttgcacg ccgcgccgac cacctgaagg ccggcgtggc
     2941 gcaagaacgg aagcttaagg caagcggcat cgatgccggc ggccagacga gccatcgccc
     3001 ggatccccgg catgaagcga aacagccgat tgaacacgag cacatcgctg ttcatgaaga
     3061 gacagaagcc gacaaaaccg tgcgtcgtcc aatggcgcag cgacaggccg ctcgctgcca
     3121 gcactggcgc agtctcgcgg tagagaagcg gccgctcggt ctcgtgatcg agcatcggcg
     3181 agaggcgata gacgagagcg cggatccagc gccacagcgc gaaatcgctc accggctcgc
     3241 gaaagtagaa ccagccgccc ggcttcagga tgcgcgcgac ctccgcgaag agcgcgcggc
     3301 gatcgctcac atgatggatg ccgccgagca tgaagacggt atcgaaggcg tcgctcgcga
     3361 gcggcagtcg cgtcgcgtcg ccctggacga aggagagatg gcgggtggta ttgtcgcgcc
     3421 gcgccgcttc gagcatcgaa accgagatgt cgacgccgat gccgcggccg atcctggcgt
     3481 cgaacagctg gaacgcctcg gcgtggccgc agcacagctc ggcgaccgtg ccgagccgct
     3541 cgccggggat cgccgcaaag agggcgcgat cgagcgatgc catgtattcc tgcgtatggg
     3601 gataggcgag gttctcgata taggctgcgg cgatcttctc gtaatgcgcc tcttggatac
     3661 gcgcgtgatc ggagcagaac cgctcggcga agagcggaat gccgctctcg gtgaccgcgt
     3721 agacgcggtc tcccgaggcg ctctgcagcc ggtcgccccg ctgcaccagc tgcgctcccg
     3781 tctcggggca gcgcagcagc ggcaggatgt cgctcagcgc ggcgccgcga acggcgatcg
     3841 tcggagcggc gatcatggtt gcggccggcc gctcgcggcc gcagcgggct tggtcccgat
     3901 cgccgaccag gacacctggt tgacccagga gaggcggatg tcgacgaggc cgctcgcgcg
     3961 catcagcgcc tcgaccgtcg cgcgcggcca ataatgggcg atcttcggca gcatctggtc
     4021 gaagacgatc gagcggagat gcctgaattg gagccggcgc agcaggcgga aatattcgtt
     4081 cgaatcgagg ccgaggcgca gcgcgagcca gagcgcgacc gagggataga gcgacagatg
     4141 atggaccatg ccgatgggca gccggctgaa gagcagcttg cgcaagggcg tcagcacatc
     4201 aacgacgaga ccgttgttct cgcgcccata gacccagatc agcaccttgc cgccgcgcct
     4261 ggccgccgtc accatggccg ccagggcctt ctccggcgcc tcgagatgat ggatgacgcc
     4321 gatcgagaag acgatgtcga agcgctcgat gtcaccgatc gcataggcgc tgcaggcgcg
     4381 gacctccacc ttcgggcagg aggagagcgt ccgtcgcgcc gacgccagcg agcgctcatc
     4441 gacatcgatc gcgacaccgc ccgccgcacc gtaggtcatc ggccagtaac tgttgcggcc
     4501 catgccgcag ccgacatcga ggaacagttt gccgcgccaa tcctcgggcg agagatgcac
     4561 ggtccagcgg cggaactgct cctcgtattc ggcgcgcatc tccgcgtagg tgtgccattc
     4621 atagccgaag cgttccggcg atccggcggc gacgg
//
LOCUS       DASYNS010000218        14107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5351421, whole genome shotgun sequence.
ACCESSION   DASYNS010000218 DASYNS010000000
VERSION     DASYNS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5351421"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1358
                     /locus_tag="VGP50_06780"
     CDS             261..1358
                     /locus_tag="VGP50_06780"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV8027116.1"
                     /translation="MGTFRFLLAATVMLGHYFPVYPQPLPFVMYGTASVPAFYIVSGF
                     LITLVLEERYGGRIGLFYSNRALRIFPLYWAALALFLLVNFLVAGGHLPQSPAYHSTS
                     ALWWTQQHGGQVGIIGTMMLALLNIFILGQDLIIYIGQWPHVASRDFFYHGFMYVGPA
                     WTVAVEFAFYAVAPFVVRRPRWTAAVLVASLSARVIAIACGYTEFIDSYGFAPFEMAF
                     FMGGSLAYRAYVRLRAMNAAPVTAYAVISSVAMLLLTVFYLRLPYARPLYLIAACLCL
                     PGIVLAGRWNPADGILGELSYPVYLLHPLLSITVIPGSTRLPEVGAVVSVLALSFALV
                     FLVERPLEKLRRRRAQAPPMDVPCIPAAQQA"
     gene            complement(1373..2086)
                     /locus_tag="VGP50_06785"
     CDS             complement(1373..2086)
                     /locus_tag="VGP50_06785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV8027117.1"
                     /translation="MTNFWDKLLPDQPLHRDHFTGAPERFFPALFETITRTSGIKTPY
                     EEFDLVTTDKFTVEEMGSNPVALRFLQLLIRLSRARRVLEIGAFIGVSAMYMAKALPS
                     GGELVTIEKFDHFADICRRNFERNGLADRITLLQGDAHAVIDGLPRAKPFDFMFIDGN
                     KERYAEYMRRLEPLLAPGGLMIVDDALFHGDALNAKPTTEKGQGARDALDLAAGWTGY
                     CRVLLPVANGMLMLMKNPV"
     gene            complement(2130..2999)
                     /locus_tag="VGP50_06790"
     CDS             complement(2130..2999)
                     /locus_tag="VGP50_06790"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8027118.1"
                     /translation="MHVLVTGASGFVGSHLVRDLAARGFAVTGFYRREPGLAADLPST
                     PNVTLVKTDLAALEALPKRCDAVVHAAASSAWTGISVDAIVRDNVVATRRLLDLAEHA
                     GCAGFIFLSSLSYYGTIEAAEVDEATPIRDPDVYGASKYLGERMLCDRRERLPGLALR
                     LPGVVGRGARRNWLAGTAERFRRGEAAAVFNPDAAFNNAVHVADLAAFVARVLERSWA
                     GFDAMVLGARGRLTVRGVAERLAAGMGVKARIDVVAAPKPAFTLSSARAIERWGYDPM
                     EIGAVVDRFAGEA"
     gene            complement(3021..3932)
                     /locus_tag="VGP50_06795"
     CDS             complement(3021..3932)
                     /locus_tag="VGP50_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEV8027119.1"
                     /translation="MRNTFSEALYQEATENPDIYIVVADISPAGSMSKFSAQYPERFI
                     NVGVAEQSMIGICAGLALKGCQPFAYTIATFSLYRPFEMVRDDLCYQNLPVTVVGMGA
                     GVIYSTLGGTHHTMEDIAIAGALPNMQIVAPCDPAECTEATRWCARQKNGPVYLRIGK
                     AGEPDLTKGAESWRFGRLRYLRRGSDVCILSYGVITKMAVELADHLAGQGKSVSVVSA
                     HTVKPLDREGVIEALQQHKHVIVLEENVPQGGLAPQTKQIAWDIAAKCRLDTFTLQDR
                     FIHNYGSHNDLLAAHGLSVERLLRAVR"
     gene            complement(3929..4753)
                     /locus_tag="VGP50_06800"
     CDS             complement(3929..4753)
                     /locus_tag="VGP50_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV8027120.1"
                     /translation="MARGSNSFDGEAARERCRRFRRRILDISQQVSALHIGSAYSCTE
                     IVDCIYYGLMRRNADGSSPDTFLMSKGHGCMIQYVILEDQGILSRADLDAYCTPEGRL
                     GVHPDYGNPGIAAATGSLGHGLSMVCGMALAERGRKTGGIVYTVLSDGEVQEGSTWEA
                     TMMASSLGLDNIVAFIDNNDFQSLGRTSETHPSFYPVVDKFRAFGWETVAVEGHDSRA
                     LFEAVAGRQGGKPLMVVAKTTKGKGVSYMENVPIWHYRSPNKEEYQTAVRELEAMR"
     gene            complement(4798..5451)
                     /locus_tag="VGP50_06805"
     CDS             complement(4798..5451)
                     /locus_tag="VGP50_06805"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8027121.1"
                     /translation="MREVNLLAKLPKGKRNLNARAAKTEEHVRVSREYGEAYFDGPRE
                     YGYGGYRYDGRWIPVAEDMIAHFGLKPGDRVLDIGCAKGFLVKDLMTACPGLQAFGVD
                     ISRYALMHCEPEVVGRLHLGNALDLPFPDGSFQAVISLNTVHNLERADCMRALREIER
                     LAPGKGFVQVDSYRTPEQRALFVDWVLTARYHDYPDGWIRTFKDAGYSGDYYWTIIE"
     gene            complement(5451..6338)
                     /locus_tag="VGP50_06810"
     CDS             complement(5451..6338)
                     /locus_tag="VGP50_06810"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV8027122.1"
                     /translation="MLEHRDPAGTSPARVVIVGAGGFIGSTIARLVAAEGVPLLALTR
                     KDADLLAPEGEQKLRERLRAADSVVFVSAIAPAKTTAMLMQNLQMADAACRAFAAVPP
                     AHLLYVSSDAIYADDANPVTERSAAAPSTIHGMMHAAREMMLKNASPAPFAALRPTLI
                     YGAADPHNGYGPNRFRRQAAKGEAIAIFGDGEERRDHVHVEDVARLALLMLRHRSVGA
                     LNAVTGRSVSFHDVAHLVAAQFTPRAVVASVPRPGPRPHLTHRHFDITQCLKAFPGFR
                     YTELAQGIARVHRETVERG"
     gene            complement(6331..7623)
                     /locus_tag="VGP50_06815"
     CDS             complement(6331..7623)
                     /locus_tag="VGP50_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV8027123.1"
                     /translation="MTLPRIGFAGMTHLGLVSGVAAAARGFPTVCFDPDAALIAALER
                     ERLPVVEPDLAELLAAHRGRLGFSADPQALAACDLVYVAPDVPTDDSGGSDLAPLEAL
                     LTLVEGAARPDAVLVVLSQVPPGFTRTRLRPGRRLYYQVETLIFGRAVERATRPERVI
                     IGCVDPAEKLPRPYQEFLASFECPILPMRLESAELAKISINLCLVASVSVANTLAELS
                     EGIGADWSEIAPALKLDRRIGTFAYLSPGLGLAGGNLERDLATVMRLSRETGSDAGVV
                     DAFLRSSRHRRDWVLRLLHHEVFASRPQALLGILGLAYKENTHSTKNSPALALIEHLG
                     PWRLRLYDPVVPATAARHLRATAAASAVDAASAVDALVIMTPWPAFRELPLRDIAGRM
                     AGRSLIDPYRVVDGKAAVAAGLDYFTLGVPPARGSRHA"
     gene            complement(7620..8552)
                     /locus_tag="VGP50_06820"
     CDS             complement(7620..8552)
                     /locus_tag="VGP50_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV8027124.1"
                     /translation="MRVIVVGMGVQGRKRLLVAGKDAVATVDPVNPEAQYRAVADVPP
                     SAYDAALVCTPDEPKLALLDDLLARGKHVLVEKPLFAESDEAIKRLEKVARSNRAVCY
                     TAYNHRFEPHYVRMRDLLHSGDLGRLYRCRMFYGNGTARLVRDSAWRDQGAGVLPDLG
                     SHLLDTLRFWFGDVGDDFRVASASCFENRAPDHVVLSSETARPKLELEMTLLSWRNHF
                     TCDVFAERGSAHIRSLCKWGPTTFTRRQRVLPSGRPPEETVTLVQDDPTWALEYAHFK
                     ALCAAPEPADLGNDLWLNHLLRKLGRDALRDAAG"
     gene            complement(8585..10132)
                     /locus_tag="VGP50_06825"
     CDS             complement(8585..10132)
                     /locus_tag="VGP50_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV8027125.1"
                     /translation="MIEVNFRSKVKTVEELTAIIGARPRAKKVIMCHGTFDIVHPGHI
                     RHLMYAKSKADILVASLTSDRHIAKANFRPYVPEQLRAMNLAVLEMVDYVIIDSEPKP
                     LRNLEIIQPDYFAKGYEYVRGGVDPRTQEELEILERYGGEFIYTPGDIIYSSSAIIEA
                     QPPDLSVEKLMVLLKGEDLSFDHLRKAIYQLEGVKVHVVGDTIVDSYTYTTLIGGNSK
                     TPTFSVRFDRQLDYVGGAGIVAKHLRAAGAKVAFTTLLGEDALGHFVLDDLKANDIEV
                     NAIIDPTRPTTNKNAFIADNYRLLKVDKVDNHSISDKIQHQLEEHIRKTRTQAVVFSD
                     FRHGIFNRQTIPPLIAALPRGAFRVADSQVASRWGNILEFTDFDLITPNEREVRFALG
                     DQDSVIRPLGLELFRQARCKTLILKMGARGLITYRAMPKAAEDVRAFFVVDSFAQNIV
                     DAVGAGDALLAYATLSMVATGNAVIASILGSLAAAVECEHDGNSPVRPRQVLEKLESV
                     ARDAQLG"
     gene            complement(10129..11148)
                     /locus_tag="VGP50_06830"
     CDS             complement(10129..11148)
                     /locus_tag="VGP50_06830"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEV8027126.1"
                     /translation="MTLRPIAIVTGGAGFIGSHMVDLLLARGYRVRVIDNLVGGREAN
                     LAHHAGNADLAFERRDIRAVAPGDPLFADARYVFHFAGIGDIVPSIERPIEYMDVNVQ
                     GTVCMLEAARTAALRKFVYAASSSCYGLAQVPTREDHAIDPKYPYALSKYLGEVAGFH
                     WQKVYRLPFNAIRIFNAYGTRSRTSGAYGAVFGVFLRQKLAGKPFTVVGDGTQQRDFL
                     YASDIAEAFLMAAETPLAGDVYNLGAGNPQSVNRLVELLGGAVLRIPKRPGEPDCTWA
                     DIGKITAALGWRPKVSFEEGVGRILENIDYWREAPLWDAQSIQQATRTWFTYLGETSE
                     AEVQR"
     gene            complement(11145..12158)
                     /locus_tag="VGP50_06835"
     CDS             complement(11145..12158)
                     /locus_tag="VGP50_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8027127.1"
                     /translation="MNPNGIAKVLITGGAGYVGNVLVPQLLGAGYEVIVYDIMFFGCD
                     TLPKHPKLTIVDGDIRDTAMLASRMKGVDAVLHLACIANDPSFDLDPTLGTSINFDCF
                     EPMVTAARKAGVRRFVYCSSSSVYGVSEKPDVTEEHPLVPLTDYNKFKGLCEPLLFKH
                     QAPGFTCVIIRPATVCGYSPRTRLDLSVNILTNLAVNKGKITVFGGEQMRPNLHVNDM
                     CDAYQMLLEVPAEKIAGQIFNCGYQNLKIKDIALVVRKVVAEEFPERGPVEIETTPSN
                     DLRSYHINSDKIRRVLGFVPKHTVADAVRDLCRAFKAGKLPNSLEDDRYFNVRRMKVM
                     KAA"
     gene            complement(12187..12930)
                     /locus_tag="VGP50_06840"
     CDS             complement(12187..12930)
                     /locus_tag="VGP50_06840"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEV8027128.1"
                     /translation="MRTVSDLRVKIFADGADLASIAKLAENPLIKGFTTNPTLMRKAG
                     VTEYAAFARDVLRIVGDRPVSFEVFADAFDEMEAQALEIASWRANVNVKIPVSNTKGA
                     FCGPLIRRLSQAGVTVNVTAVMTLDQVGAVTECLAPRTPAIVSVFAGRIADTGRDPVP
                     LMAEAVRILQARPRAELIWASPRELLNVFQADDVGCHIITATPDVLSKLSLVGKDLAA
                     YSLETVEMFYRDAQAAGFAIATAPVRAVG"
     gene            complement(12918..13472)
                     /locus_tag="VGP50_06845"
     CDS             complement(12918..13472)
                     /locus_tag="VGP50_06845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV8027129.1"
                     /translation="MSSAAVFLDRDGTINTAAIRDGKPYPPDSIDDLVIVPGASGALL
                     QLRRAGYLMVMVTNQPDVATGRQGRAVVEAINEAIRGQLPLDDIRVCYHLDADACHCR
                     KPKPGMLLDAARDWGIDLSRSFIVGDRWRDVEAGRAAGCRTVLIEAGYEERRTVPDFS
                     AQSLVDACEIILRVGASRRRSCGP"
     gene            complement(13469..14071)
                     /locus_tag="VGP50_06850"
     CDS             complement(13469..14071)
                     /locus_tag="VGP50_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV8027130.1"
                     /translation="MTHVATYFEQVAGIAAKIDHNAIERMAAGLAELRERGGRLFIIG
                     VGGSAANCSHAVNDFRKLCGIEAYSPVDNVSELTARTNDEGWETVFAAWLRTSRAGSK
                     DAILVFSVGGGDAERNISANIVRALEEAKSRALKIYGVVGRDGGFTKKVGDDVVVVPT
                     LDPKTVTPHTEAFQAVVWHCLVSHPSLAIKEAKWEGLAQK"
BASE COUNT         2356 a         4612 c         4696 g         2443 t
ORIGIN      
        1 cccgtcgctc ccggcgatgc tggcgccggg cgccgagcat catttgctcc atttcatcga
       61 aaaccatgtc aaaccgcgat cggcaggaat aatcgagcgg ccgacggcgc ggatcggcga
      121 gcatcggctt tacgtggtcg acgaattgcg cggtgcttgg tcgctgcccg ttggagcgcg
      181 tacgcccgcg ctcactcttg cgtgaaccgg cagcagacag gctcctaggc caagggcttc
      241 acgcggaggc atcgggagac atggggacat tccggtttct gctcgcggcg acggtgatgc
      301 tcggccatta ctttccggta tatccgcagc ccctgccttt cgtgatgtat ggcacggcgt
      361 cggtgccggc gttctacatc gtctccggat ttctcatcac gctcgtcctg gaagagagat
      421 atggcgggcg tatcggcctc ttctattcca atcgggcgct gcgcatcttc ccgctctatt
      481 gggccgccct cgcccttttc ctgctcgtca actttctcgt cgccggggga catcttccgc
      541 aatctcccgc ctaccattcg acgagcgcgc tctggtggac acagcaacac ggcggccagg
      601 tcggcatcat cggcacgatg atgctggcgc tcctcaacat cttcattctg ggccaggacc
      661 tgatcattta tatcgggcag tggccgcatg tggcgtcgcg cgattttttc tatcacggct
      721 tcatgtatgt cgggcccgca tggacggtgg ccgtcgaatt cgcgttctat gccgtggccc
      781 cgttcgtggt gcgccggccg cgctggaccg cggctgtgct cgtcgcgtca ttgagcgccc
      841 gcgtcatcgc catcgcctgc ggctacacgg aattcatcga ttcatacggc ttcgcgccgt
      901 tcgagatggc ttttttcatg ggcggctccc tcgcctaccg cgcctatgtg cgattgcggg
      961 cgatgaacgc ggcccccgtc accgcctatg cagtgatctc cagcgtggcc atgctgcttc
     1021 tgacggtgtt ctatctgagg ctgccatacg cccggccgct ttacctgatc gcggcgtgcc
     1081 tatgcctgcc cggaatcgtc ctggcgggac ggtggaatcc agcggacgga atcctgggcg
     1141 agctctccta ccccgtctat ctgctccatc cgctgctgtc gatcaccgtc atacctggaa
     1201 gcacgcggct tccggaagtc ggcgctgtcg tcagtgtctt ggcgctttcc ttcgcactgg
     1261 tattcctggt cgagcgcccc ctcgaaaagc tgcggcgccg gcgcgcgcaa gccccgccaa
     1321 tggacgtgcc gtgcattccc gccgcccagc aggcgtagct gctcgccctc ggctacaccg
     1381 gatttttcat cagcatcagc atcccattcg ccaccggcaa cagcacccgg caatacccgg
     1441 tccagcccgc ggcgagatcg agcgcatcgc gcgcgccctg ccccttctcc gtcgtcggct
     1501 tggcgttgag cgcgtcgcca tgaaagagcg catcgtcgac gatcatcagc ccgcccggcg
     1561 ccagcagcgg ctcgagccgc cgcatatact cggcgtagcg ttccttgttg ccgtcgatga
     1621 acatgaaatc gaagggcttg gcgcgcggca gcccgtcgat gaccgcatgc gcgtcgccct
     1681 gaaggagcgt gatgcggtcg gccagcccgt tccgctcgaa gttccgccgg cagatgtcgg
     1741 cgaaatgatc gaacttctcg atggtcacga gctcgccgcc ggacggcagc gccttcgcca
     1801 tgtacatggc ggagacgccg atgaacgcgc cgatctcaag gacgcgccgc gcccgcgaca
     1861 ggcggatcag cagctgcagg aagcggagag ctacgggatt cgatcccatc tcctcgacgg
     1921 tgaacttgtc ggtcgtgacg agatcgaact cctcgtaagg cgtcttgatg ccgcttgtcc
     1981 gcgtgatggt ctcgaacagc gccgggaaga agcgctcggg cgcgcccgtg aaatgatcgc
     2041 ggtgcaaggg ctggtcggga agcagcttgt cccaaaaatt cgtcatcgga atctcctacc
     2101 tgcctgccgc atcctcgcca tggcgcccgt caggcctcgc cggcgaagcg gtcgacgaca
     2161 gcaccgatct ccatgggatc gtagccccat cgttcgatcg cccgcgccga ggagagcgtg
     2221 aaggccggct tcggcgctgc caccacgtcg atcctggctt tgacgcccat gcccgcagcg
     2281 aggcgctcgg ccacgccgcg gacggtgagc cggccgcggg cgcccagcac catggcatcg
     2341 aaaccggccc agcttcgctc gagcacgcgc gcgacgaacg cggcgagatc ggcgacgtgg
     2401 acggcgttgt tgaaggcggc atcggggttg aagacggctg ctgcttcgcc gcggcggaag
     2461 cgctctgccg tgcccgcgag ccaattgcgc cgcgcgccgc gcccgacgac gccgggaagg
     2521 cggagcgcca gccccggcag ccgctcccgg cggtcgcaaa gcatgcgctc gcccagatat
     2581 ttgctggcgc cgtagacatc cggatcgcgg atgggcgtcg cctcgtccac ctcggccgcc
     2641 tcgatcgtcc cgtaatagga cagcgacgac aggaagatga agccggcgca tcccgcatgc
     2701 tcggcgagat ccagcaggcg ccgcgtggcg acgacgttgt cgcgcacgat cgcgtcgacg
     2761 gagatgccgg tccaggccga gctcgccgcc gcgtgcacga cggcgtcgca acgcttgggg
     2821 agcgcctcca gcgcggcgag atcagtcttg accaaggtga cgttgggcgt gctcggcaga
     2881 tccgcggcga ggcccggctc gcgccgatag aagccggtga cggcaaagcc ccgcgccgcc
     2941 aggtcgcgca cgagatgcga gccgacgaag ccgctggcgc cggtgacgag gacatgcatc
     3001 ggcccggccc gcggctccgc tcagcggacg gcgcgcaaca agcgctcgac gctcaagcca
     3061 tgcgccgcca gcagatcgtt gtggctgccg tagttatgga tgaagcggtc ctgcagcgtg
     3121 aaggtgtcga ggcggcattt ggcggcgata tcccaggcga tctgcttggt ctgcggcgcg
     3181 agcccgcctt gtggcacatt ctcctcgagg acgatcacgt gcttgtgctg ctgcagcgcc
     3241 tcgatcacgc cctcgcggtc gagcggcttc accgtatggg cggagacgac ggagacggat
     3301 ttgccttggc ccgccaggtg gtcggcgagc tcgacggcca tcttcgtgat gacgccgtag
     3361 gagaggatgc agacatcgct gccgcggcgc agatagcgca gccggccgaa gcgccaggac
     3421 tcggcgccct tcgtcagatc gggttcgccc gccttgccga tgcgcagata gaccgggccg
     3481 ttcttctgcc gcgcgcacca gcgtgtcgcc tccgtgcatt cggcgggatc gcagggcgcc
     3541 acgatctgca tgttcgggag cgcgccggcg atggcgatgt cctccatggt gtggtgcgtc
     3601 ccgcccaagg tcgagtagat gacgcccgca cccatgccga cgaccgtgac cggcaagttc
     3661 tgatagcaga gatcgtcgcg caccatctcg aagggacggt agagcgagaa cgtcgcgatg
     3721 gtataggcga agggctggca gcccttgagg gcgagcccgg cgcagatgcc gatcatgctc
     3781 tgctcggcga cgccgacatt gatgaagcgc tcggggtatt gcgcactgaa tttcgacatg
     3841 ctaccggccg gcgagatgtc ggcgacgacg atgtagatgt cggggttctc agtcgcttcc
     3901 tggtaaagcg cctcggagaa ggtgttcctc accgcatcgc ctccaattcg cggaccgcgg
     3961 tctgatattc ctccttgttg ggcgagcgat agtgccagat cggcacattc tccatgtagc
     4021 tcaccccttt gcccttcgtg gtcttggcca ccaccatgag cggcttgccg ccctgccgcc
     4081 cggccaccgc ctcgaagagc gcacgcgagt catgcccctc gaccgcgacc gtctcccagc
     4141 cgaaggcgcg gaacttgtcg accacgggat agaagctcgg atgcgtctcc gaggtgcgtc
     4201 cgaggctctg gaaatcgttg ttgtcgatga aggcgacgat attgtcgagg ccgagcgagg
     4261 acgccatcat cgtcgcctcc caggtcgatc cctcctgcac ctcgccgtcg ctcaagacgg
     4321 tgtagacgat gccgccggtc ttgcggccgc gctcggccag cgccatgccg cagaccatgg
     4381 agaggccgtg gccgagcgac ccggtggcgg cggcgatgcc gggattgccg taatcgggat
     4441 gaacgccgag cctgccctcg ggcgtgcaat aggcgtcgag atcggcccgg ctcaggatcc
     4501 cctgatcctc gaggatcacg tattggatca tgcagccatg gcccttggac atgaggaagg
     4561 tatcgggcga agagccgtcg gcattgcgcc gcatgaggcc gtaatagatg cagtcgacga
     4621 tctcggtgca ggaataggcc gagccgatat ggagcgccga cacctgctgc gagatatcga
     4681 ggatgcgccg gcggaagcga cggcagcgct cgcgcgccgc ttcgccgtcg aagctgttgc
     4741 tgcctctggc catgctgccg atcccgctca ttcgatgccc gctcattcga tgcccgctca
     4801 ttcgatgatc gtccaataat agtcgccgct gtagcccgcg tccttgaagg tccggatcca
     4861 accgtcggga tagtcgtgat acctggcggt gaggacccag tcgacgaaga gcgcccgctg
     4921 ctccggcgtg cgataggaat cgacctgcac gaagcctttg ccgggcgcca gccgctcgat
     4981 ctcgcgaagg gcccgcatgc agtccgcgcg ctccaggttg tgcaccgtgt tgagcgagat
     5041 caccgcctgg aagctgccat ccgggaacgg caggtcgagc gcgttcccga gatgcagccg
     5101 ccccaccacc tcgggttcgc aatgcatgag cgcatagcgc gagatgtcga cgccgaacgc
     5161 ctggagcccg ggacacgccg tcatcaggtc cttgaccagg aaccccttgg cgcagccgat
     5221 atcgagcact ctgtcgccgg gcttgagccc gaaatgcgcg atcatgtcct ccgccaccgg
     5281 gatccaacgg ccgtcatagc gatagccgcc atagccgtat tcgcgcggac catcgaaata
     5341 ggcctcgcca tattcgcggg agacgcggac atgctcctcg gtcttggcgg cccgggcgtt
     5401 caggttgcgc ttgcccttgg gcagcttggc cagaagattc acctcgcgca tcagcccctc
     5461 tccaccgtct cgcggtgcac ccgggcgatc ccttgggcca gctccgtgta gcggaagccc
     5521 gggaaggctt tcaagcattg ggtaatgtcg aagtggcgat gcgtcagatg gggccgcgga
     5581 cccggccgcg gtacgctcgc caccaccgcc cgcggcgtga actgggccgc caccagatgc
     5641 gcgacgtcgt ggaacgatac cgagcgtccc gtcacggcgt tgagcgcgcc cacgctgcga
     5701 tggcgcagca tcagcagggc gagccgcgcg acgtcctcga catgcacatg gtcgcgccgc
     5761 tcctcgccgt cgccgaaaat ggcgatcgcc tcgcctttcg cggcctggcg gcgaaagcgg
     5821 ttcgggccat agccgttatg cgggtcggcc gcgccgtaaa tgagggtcgg ccggagcgcc
     5881 gcaaagggcg ccggcgacgc gttcttcagc atcatctcgc gcgccgcatg catcatgccg
     5941 tggatggtcg agggcgcggc ggccgagcgc tccgttaccg gattggcgtc gtcggcatag
     6001 atcgcgtccg agctgacgta gaggagatgc gccggcggca ctgcggcaaa agcgcggcag
     6061 gccgcgtcgg ccatctgcag gttctgcatc agcatggccg tggtcttcgc cggcgcgatg
     6121 gcggagacga agacgacgct gtcggccgcc cgcaggcgtt cgcgcagctt ttgctcgccc
     6181 tcgggcgcga ggagatcggc gtccttgcgc gtgagcgcca ggagcgggac gccctccgcc
     6241 gcgacaagcc gggcaatggt gctgccgatg aagccgccgg cgccgacgat gacgacgcgg
     6301 gcgggggacg tgccggcggg atcgcgatgc tcaagcatgg cgcgatccgc gcgccggcgg
     6361 gacgcccagg gtgaaatagt cgaggccggc cgcgacggcg gctttgccgt cgaccacgcg
     6421 gtaggggtcg atcaggctgc gccccgccat ccgtccggca atgtcgcgca gcggcagctc
     6481 gcggaaggcg ggccacggcg tcatgatcac cagcgcatcg acggcgctcg ccgcatcgac
     6541 cgcggaggcg gcggccgtgg cccgaaggtg acgcgcggcc gtcgccggga cgacggggtc
     6601 atagagcctg agccgccagg ggccgagatg ctcgatcagc gccagcgccg gcgagttctt
     6661 ggtcgaatgc gtgttctcct tgtaggcgag gccgaggatg ccgaggagcg cctgcggccg
     6721 gctggcgaag acctcgtggt gcaggaggcg cagaacccag tcgcgccggt gccggctgct
     6781 gcgcaggaag gcgtcgacca cgccggcatc gctcccggtc tcccgggaga gccgcatcac
     6841 ggtcgcaaga tcgcgttcga gattgccgcc cgcgagaccc aggcccgggc tcagataagc
     6901 gaaggtgccg atccggcggt cgagcttcaa cgccggcgcg atctcgctcc agtcggcgcc
     6961 gatcccctcc gagagctcgg ccagcgtgtt ggcgacgctc accgaggcga cgaggcagag
     7021 attgatcgag atcttggcga gctcggcgct ttcgaggcgc atcggcagga tcgggcactc
     7081 gaagcttgcg aggaactcct ggtaagggcg cggcagcttc tccgcgggat cgacgcagcc
     7141 gatgatcacc cgctcgggcc gggtcgcccg ctcgacggcg cggccgaaga tgagggtctc
     7201 gacctggtag tagagccgcc ggcccggccg cagcctggtt cgcgtgaagc ccggcggcac
     7261 ctggctcagg acgaccagca cggcatccgg ccgcgccgcc ccctcgacca gcgtcaacag
     7321 cgcctcgagc ggcgcaaggt cgctgccgcc gctatcgtcg gtgggcacgt cgggcgccac
     7381 atagacgagg tcgcaagcgg cgagcgcctg cggatcggcg ctgaagccaa ggcgcccgcg
     7441 atgcgccgcc agcagctcgg cgaggtccgg ctcgaccacg gggagccgct cccgctccaa
     7501 ggcggcgatc aacgctgcgt ccgggtcgaa gcagaccgtg gggaatcccc gtgccgctgc
     7561 tgcgaccccg gacacgaggc ccagatgcgt cattccggca aagccgatgc gcggcaaggt
     7621 catcctgcgg cgtccctgag cgcatcgcgg ccgagcttgc ggagaaggtg gttgagccag
     7681 agatcattgc cgagatcggc gggctccggc gcggcgcaca gcgccttgaa atgcgcgtat
     7741 tcgagcgccc aggtcggatc gtcctggacg agcgtcaccg tctcctccgg cggccggccg
     7801 ctcggcagga cgcgctgccg ccgcgtgaag gtcgtcggcc cccatttgca gagcgagcgg
     7861 atatgagcgc tgccccgctc ggcaaagacg tcgcaggtga aatggttgcg ccaggagagc
     7921 agcgtcatct ccagctcgag ctttggccgc gccgtctcgg acgagagcac gacatgatcg
     7981 ggggcgcggt tctcgaagca gctcgccgac gcgacgcgaa agtcgtcccc gacatcgccg
     8041 aaccagaagc gcaatgtgtc gaggagatgg gagccgaggt ccggcaggac gccggcgccc
     8101 tggtcgcgcc aggcggaatc gcggacgagg cgcgccgtgc cgttgccgta gaacatccgg
     8161 cagcgataga gccggcccag atcgcccgaa tgcaagaggt cacgcatgcg cacataatgc
     8221 ggctcgaagc ggtgattgta ggcggtgtag cagacggcgc gattgctgcg ggcgaccttc
     8281 tcgagccgct tgatcgcctc gtcgctctcg gcgaaaagcg gcttctcgac cagcacatgc
     8341 ttgccgcgcg cgagcaggtc gtcgaggagc gcgagcttcg gctcgtccgg cgtgcagacc
     8401 agcgccgcgt cataggccga gggcggtacg tccgcgacgg cccgatattg cgcctcgggg
     8461 ttgacgggat cgaccgtcgc gacggcatcc ttgcccgcga ccagcagccg cttgcgtccc
     8521 tgcaccccca ttccgacgac gatgacgcgc atcgggccgc tatcccaccc ctgccccgcc
     8581 gacactagcc gagctgcgcg tcccgggcga cgctctcgag cttctccagg acctgccgtg
     8641 ggcggaccgg actgttgccg tcatgctcgc attcgaccgc cgcggcgagc gatcccagga
     8701 tcgaggcgat gaccgcattg ccggtcgcca ccatcgacaa cgtcgcatag gcgaggagcg
     8761 cgtcgccggc gccgacggca tcgacgatgt tctgtgcgaa gctgtcgacg acgaagaagg
     8821 cgcgaacgtc ctcagccgcc ttgggcatcg cccgataggt gatgagcccg cgggcgccca
     8881 tctttaggat cagcgtcttg cagcgcgcct gccggaagag ctcgaggccg agcgggcgga
     8941 tgaccgaatc ctggtcgccc agggcgaagc gcacttcgcg ctcgttgggc gtgatgagat
     9001 cgaaatccgt gaattcgagg atgttgcccc aacggctcgc cacctggctg tccgcgaccc
     9061 gaaaggcgcc tcggggcagc gcggcgatga gtggcggaat ggtctggcgg ttgaagatgc
     9121 cgtgccggaa atcgctgaac accacggctt gcgtgcgcgt cttgcgaata tgctcctcga
     9181 gctgatgctg gatcttgtcc gagatcgaat ggttgtcgac cttgtcgacc ttaagcagcc
     9241 gataattgtc ggcgatgaac gcgttcttgt tggtggtggg gcgcgtcgga tcgatgatcg
     9301 cgttcacctc gatgtcgttc gccttgaggt cgtcgagcac gaaatggccg agcgcgtcct
     9361 cgcccagcag cgtggtgaac gccaccttcg cacccgcggc ccgcagatgc ttggcgacga
     9421 tgccggcgcc gccgacatag tcgagctggc ggtcgaagcg cacgctgaag gtcggcgtct
     9481 tgctgttgcc gccgatgagt gtcgtatagg tatagctgtc gacgatggtg tcgccgacga
     9541 catgcacctt gacgccctcg agctgataga tggccttgcg caaatggtcg aagctcagat
     9601 cctcgccctt cagcaggacc atcagcttct cgacggaaag gtcgggcggc tgcgcttcga
     9661 tgatcgccga ggaggagtag atgatgtcgc ccggcgtata gatgaattcg ccgccatagc
     9721 gctcgagaat ctcgagctcc tcctgcgtgc gcggatcgac gccgccgcgc acatactcgt
     9781 agcccttggc gaaatagtcg ggctggatga tctcgagatt gcgcagcggc ttgggctcgc
     9841 tgtcgatgat gacgtagtcc accatctcga gaacggcgag attcatcgca cgcagctgct
     9901 ccgggacata ggggcggaaa ttcgccttgg cgatatggcg gtcgctggtc aggctcgcca
     9961 ccaggatgtc cgccttgctt ttggcataca tcaggtgccg gatgtggccg ggatggacga
    10021 tgtcgaaggt gccgtggcac atgatcacct tcttggcgcg cggccgcgcg ccgatgatcg
    10081 ccgtgagctc ctcgaccgtc ttgaccttgc tgcggaagtt cacttcgatc atcgttgtac
    10141 ctcggcctct gaggtctcgc ccagataggt gaaccaggtc cgtgtggcct gctggatcga
    10201 ctgcgcgtcc cagagcggcg cctcccgcca atagtcgatg ttctcgagaa tgcgccccac
    10261 accctcctcg aagctcacct tgggccgcca gccgagcgct gcggtgatct tgccgatgtc
    10321 ggcccaggtg cagtcgggct cgcccgggcg cttcgggatg cgcaggacgg cccctcccag
    10381 cagctcgacc aggcggttca cgctctgcgg attgcccgcg cccagattat agacgtcgcc
    10441 cgcgagcggc gtttcggctg ccatgaggaa cgcctcggcg atatcggagg cgtagagaaa
    10501 atcgcgctgt tgcgtgccgt cgccgacgac ggtgaagggc ttgccggcca gcttctggcg
    10561 caggaagacg ccgaacaccg cgccataggc gcccgaggtc ctggaacgcg tgccataggc
    10621 attgaagatc cggatggcgt tgaacggcag gcgatagacc ttctgccagt ggaagccggc
    10681 gacttcgccc aggtatttgc tcagggcata cgggtatttc gggtcgatcg catggtcctc
    10741 gcgggtcggc acctgcgcca ggccatagca ggaggacgag gcggcataga cgaatttccg
    10801 cagagcggcg gtgcgcgccg cctccagcat gcaaaccgtg ccctggacgt tgacgtccat
    10861 gtattcgatc ggccgctcga tcgacggcac gatgtcgccg atgccggcaa agtgaaagac
    10921 atagcgcgca tcggcgaaga gcggatcgcc gggagcgacg gcgcggatgt cgcgccgctc
    10981 gaaggcaaga tcggcattgc cggcgtgatg ggccagattg gcttcgcggc cgcccaccag
    11041 attgtcgatc acgcgcaccc gatagccgcg ggcgagcagg agatcgacca tatggctgcc
    11101 gatgaacccc gcgccgccag tgacgatggc gatcgggcgc agcgtcatgc ggccttcatg
    11161 accttcatgc ggcgcacgtt gaagtagcgg tcgtcctcga ggctgttcgg cagcttgccc
    11221 gccttgaagg cgcggcacag gtcgcgcacg gcgtccgcga cggtgtgctt cggcacgaag
    11281 cccagcacgc gccggatctt gtccgaattg atgtgatagg accggaggtc gttgctgggc
    11341 gtggtctcga tctcgacggg gccgcgctcc ggaaattcct ccgccacgac cttgcggacc
    11401 accagcgcga tatccttgat cttcaggttc tgatagccgc aattgaagat ctgcccggcg
    11461 atcttctcgg ccggcacctc gagcagcatc tgataggcat cgcacatgtc gttgacgtgc
    11521 aggttcgggc gcatctgttc gccgccgaag acggtgatct tgcctttgtt gaccgcgagg
    11581 ttggtgagga tgttgacgga gaggtcgagc cgcgtgcgcg ggctgtagcc gcacaccgtc
    11641 gccggccgga tgatcacgca agtaaagccc ggcgcttgat gtttgaacag aagaggttcg
    11701 cacaaccctt tgaatttatt gtaatccgtc aaaggaacca gcgggtgctc ctcggttacg
    11761 tcgggcttct cgctgacgcc atagaccgag ctcgaggagc aatagacgaa gcgcctcacg
    11821 ccggccttcc tggcggcggt caccatcggc tcgaagcagt cgaagttgat cgaggtgccg
    11881 agcgtcggat cgaggtcgaa gctcggatca ttggcgatgc aggcgaggtg cagcacggcg
    11941 tccacgccct tcatccggct cgcgagcatg gcggtgtcgc gaatatcgcc gtcgacgatc
    12001 gtcaatttcg gatgcttcgg caacgtgtcg caaccgaaga acatgatgtc gtagacgatc
    12061 acctcgtagc cggcgcccag cagctgcggg acgagcacat tgcccacata tccggcgccg
    12121 ccggtgatca ggacctttgc gatgccgttc ggattcattc cctctcctcg tcgcaaattc
    12181 ttgggctcag ccgacggcgc gcaccggcgc cgtggcgatg gcgaatccgg ccgcttgagc
    12241 atcgcgatag aacatctcca ccgtctccag cgagtaggcg gcgagatcct tgccgacgag
    12301 ggagagcttg ctcaagacat ccggcgtcgc ggtgatgata tgacaaccga catcgtcggc
    12361 ctggaagacg ttcagaagct cgcgcgggct cgcccagatg agctccgctc gcggcctcgc
    12421 ctgcagaatc cgcaccgcct ccgccatgag cggtaccgga tcgcggccgg tgtcggcgat
    12481 gcggccggca aacaccgaga cgatggccgg cgtgcgcggc gccaggcatt ccgtgaccgc
    12541 cccgacctgg tcgagcgtca tgaccgcggt cacgttgacc gtgacgcccg cttgggagag
    12601 gcgtcgaatg agcggcccgc agaacgcgcc cttcgtgttg gagaccggta tcttcacgtt
    12661 gacgttggcg cgccagctcg cgatctcgag cgcctgcgct tccatctcgt cgaacgcgtc
    12721 ggcgaagacc tcgaaggaca cgggacggtc gcccacgatg cgcaagacgt cgcgcgcgaa
    12781 ggcggcatac tcggtgacgc cggctttgcg catcaacgtc ggattcgtgg tgaatccctt
    12841 gatcagcgga ttttcagcaa gcttggcgat gcttgcgaga tcggcaccgt cggcgaatat
    12901 tttgacgcgc aggtcgctca cggtccgcat gatcgcctcc ttgaggctcc gaccctgagg
    12961 atgatctcgc aggcatcaac gagcgactga gcggaaaagt ccggcacggt acggcgctcc
    13021 tcgtagccgg cctcgatgag cacggtccgg cagcccgcgg cgcgccctgc ctcgacgtcg
    13081 cgccagcggt cgccgacgat gaagctgcgg ctgagatcga tgccccagtc gcgcgcggcg
    13141 tccagcagca ttcccggctt cggcttgcgg caatgacagg catccgcgtc caggtggtag
    13201 cagacccgaa tgtcgtcgag cggcagttgc ccccggatcg cctcgttgat cgcctcgacc
    13261 acggcgcggc cttgccggcc ggtcgcaacg tcgggttgat tcgtcaccat gaccatgaga
    13321 tagcccgcac ggcgcagttg tagcaacgcc ccggacgcac ccggcacgat cacaagatcg
    13381 tcaatgctgt caggcggata gggtttccca tcgcgaatgg ccgccgtgtt gatggtgccg
    13441 tcccgatcga ggaataccgc ggcgctgctc atttttgcgc cagcccctcc catttggcct
    13501 ccttgatggc caaactcggg tgggagacga ggcaatgcca gacaacggct tggaaggcct
    13561 ccgtgtgcgg tgtgaccgtt ttgggatcga gcgtgggcac gacgacgacg tcgtcgccga
    13621 cctttttggt gaaaccgcca tcccgcccta ccacaccata gattttcagc gcccgcgatt
    13681 tggcttcctc gagggcccgc acgatattgg ccgagatatt gcgttcggca tcgccgccgc
    13741 cgaccgagaa aacgagaatc gcgtccttgc tcccggcgcg gctggtgcgc agccaggccg
    13801 cgaacaccgt ctcccagccc tcgtcgttgg tgcgcgccgt gagttccgag acattgtcca
    13861 cgggcgaata ggcctcgatc ccgcagagct tgcggaagtc gttgacggcg tggctgcaat
    13921 tggcggcgct cccgccgacg ccgatgatga aaaggcgacc gccccgctcg cgcaactcgg
    13981 ccaagcccgc agccatgcgc tcgatcgcgt tgtgatcgat tttcgcggcg attcccgcga
    14041 cttgctcgaa ataggtagct acgtgtgtca ttcgctcgaa ggaggtagtt gcactggtgg
    14101 ctcacct
//
LOCUS       DASYNS010000219         4139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3481372, whole genome shotgun sequence.
ACCESSION   DASYNS010000219 DASYNS010000000
VERSION     DASYNS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3481372"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VGP50_06855"
     CDS             complement(<1..989)
                     /locus_tag="VGP50_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV8027131.1"
                     /translation="MARTISGARETLRLGILKLTDSAPVVMAQELGLFAEERLDVAIA
                     VEPSWANIADKLAYGILDGAILLPPLAMAICLGLRGAASCPLVVPMSLSLGGNTVTLS
                     AALADALPRGTLGKGPLAVAQALATLHALRRGGGSPTLAVVHAFSTHNLLLRYWLAAG
                     GIDPERDLNLVIVPPARVVQAIQQGRIDGFCAGAPWGRVAERAGLGVTVATSHDIWRN
                     GPEKIFAVRAAWAERHPETLQALLRALLRAARICDDPARASEIAATLSHERYLDMPAE
                     LLLSSLPGGGARASQTASVFFANAATFPWRSHALWTLREMARWGFIGADLDVSA"
     gene            complement(995..1582)
                     /locus_tag="VGP50_06860"
     CDS             complement(995..1582)
                     /locus_tag="VGP50_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEV8027132.1"
                     /translation="MALRVLLADSNSERADALAEKLEATDDTEVLRARPGSSIADVVS
                     AEMPDVVIVDMALPDRDALEDIRAVNSREPRPIIMFVDRDDPGFMEEAIAAGVSSYNV
                     VGITLPDVKPIVAAAIAIFRRYRLVEQELAQAKAAIEERRTLERAKAILMRRRGIGEP
                     EAYRWLRRKAMNESRRIADVAAELIATDRSDVASS"
     gene            complement(1690..2163)
                     /locus_tag="VGP50_06865"
     CDS             complement(1690..2163)
                     /locus_tag="VGP50_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027133.1"
                     /translation="MGHHRRLLAGLLLLALAGCAQPPPFPAVAPLIPPLAPEQARIYF
                     YRDWEVSESVSSWPRIYLNGAPAGAAVPGGVFYRDVPAPETYDISVDTYGVYWYPFKE
                     VALKAGDTLFVKIESLSSWASGRHYQADTFVVAIIEPAQAEPELQGMRYVEREAQ"
     gene            complement(2167..2745)
                     /locus_tag="VGP50_06870"
     CDS             complement(2167..2745)
                     /locus_tag="VGP50_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HEV8027134.1"
                     /translation="MDRQAFFRGEFAEHETVLKATLAQCEAPFMRLLDAVGAAVRRGG
                     KIMFFGNGGSAADAQHLATELTVRYIKDRKPIAAVALTTDTSALTAIGNDLGFEQLFV
                     RQLKALGAKGDVALGISTSGKSRNVILALEAAREMGIVPAALSGGEGGLLKGVADPLL
                     IVPSRTTARIQEMHIMLGQMLCGALERELGLA"
     gene            complement(2804..3853)
                     /locus_tag="VGP50_06875"
     CDS             complement(2804..3853)
                     /locus_tag="VGP50_06875"
                     /inference="COORDINATES: protein motif:HMM:NF018249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside permease"
                     /protein_id="HEV8027135.1"
                     /translation="MEPLRRLFPLLALLALASCAASPPTVTTPAAASGSPRPVKVLII
                     SMFGPEGEVWREPLALKDEIAVPGLSPDYPTVRCNGDDVCQVTTGMGHANAAASITAL
                     VFSGRFDFRHSYFLVAGIAGIDPKIGTLGSATWARYLVDYGIAQEIDAREMPKAWPSG
                     YIGIGTTGPTVKPTLEYRTEVFQLNEDLLQWAVALSRGMTLQDSDAARAYRRHYGKLP
                     AADAPSVVQCDTAAGDTWWQGSKLGERAEQWTRLLTDGKGRYCTTQQEDNATFEALKR
                     GAGARLLDIRRVAVLRTGSDFDRPYPGQSAFASLKAISAGFSVSIANLVVAGRPLVDE
                     IVAHWERWAIDIPAR"
BASE COUNT          708 a         1381 c         1388 g          662 t
ORIGIN      
        1 gccgagacgt cgagatcggc gccgatgaat ccccatcgcg ccatctcgcg aagcgtccac
       61 agggcgtgcg accgccaggg aaaagtagcg gcattggcaa agaagaccga cgcggtctgg
      121 ctcgctcgag cgccgccacc cggcagcgac gacagcagca gctcggccgg catatcgagg
      181 tatctttcgt gcgacagcgt ggcggcgatt tccgaggcgc gagcgggatc gtcgcagatg
      241 cgcgcggcgc gcagcaacgc gcgcaacagc gcttgcaatg tctcgggatg ccgctccgcc
      301 catgcggcgc gaacagcgaa gatcttctcg ggaccgttcc gccagatgtc gtgcgaggtc
      361 gcgaccgtca cgccgagccc ggcgcgctcg gccacacgcc cccaaggcgc gccggcgcag
      421 aatccgtcga tgcgcccttg ctggatcgcc tgcaccacgc gcgccggcgg cacgatgacg
      481 aggttgagat cgcgctcggg gtcgatgccg ccggccgcca gccaatagcg caggagcaga
      541 ttatgtgtcg agaaggcatg gacgacggcc aaggtcggcg agccgccgcc gcgccgcagc
      601 gcgtgaagcg ttgccagcgc ctgcgcgacg gccagcggac ccttgccgag agtgcctcga
      661 ggcagggcat cggcaagagc ggcggacagc gttacggtgt tgccgccgag gctcagcgac
      721 atcggcacga cgagcgggca agacgcggcg ccgcgcaggc cgaggcagat cgccatggcg
      781 agcggcggca gcagaatcgc gccgtcgaga atgccgtagg ccagcttgtc ggcgatgttc
      841 gcccaggacg gctcgacggc gatcgccaca tcgagtcttt cctcggcgaa gaggccgagc
      901 tcttgggcca tgacgaccgg agccgagtcg gtgagcttca gaatgcccag ccgcaacgtc
      961 tcgcgagcgc cggatatcgt cctagccatg gccgtcaact cgatgcgaca tcggatcgat
     1021 ccgtggcaat gagctcggcg gcgacatcgg caatcctccg gctttcgttc atggcttttc
     1081 gacggagcca gcgatacgcc tcgggctcgc cgatcccgcg ccggcgcatc aatatcgcct
     1141 tggcccgctc cagtgtgcgg cgctcctcga tggccgcctt ggcctgcgcg agttcttgct
     1201 cgacaaggcg atagcggcgg aagatcgcga tcgctgccgc gacgatcggc ttcacgtccg
     1261 ggagcgtgat gccgacgaca ttgtaggagc tgacgccggc cgcgatcgcc tcttccatga
     1321 agcccggatc gtcccgatcg acgaacatga tgatcggccg cggctctctg ctgttgacgg
     1381 cgcggatgtc ttccaaggca tcgcggtccg gcagcgccat gtcaacgatg acgacgtccg
     1441 gcatctcggc gctcacgaca tcggcaatgc ttgacccggg ccgcgcgcgc agcacctccg
     1501 tgtcatccgt ggcttcaagc ttctccgcga gagcatcggc tcgctccgag ttcgaatccg
     1561 cgagcaagac ccgcaacgcc atcgaatctt cttctgtacg gtgcgaccag ccgtccttgc
     1621 agatccgtgc aaagtctgtg ccaggggagc ggcatgccgg cattgcctcg gtatggcgac
     1681 acgcccgcgc tactgcgcct cgcgttcgac gtaacgcatg ccttgaagtt caggctccgc
     1741 ctgcgccggc tcgatgatgg cgacgacgaa ggtgtctgct tgatagtggc ggccgcttgc
     1801 ccaggagctt aaggattcga tcttgacgaa gagtgtatca ccggccttga gcgctacctc
     1861 cttgaacggg taccagtaga cgccgtaggt atcgacggag atgtcatagg tttcgggcgc
     1921 cggaacatcg cggtagaaga cgccgcccgg aacggcggcg ccggcgggcg cgccattcag
     1981 atagatccgc ggccaggagg agacgctctc ggagacctcc cagtcgcgat agaagtagat
     2041 gcgcgcctgc tcgggcgcga gcggcggaat gagcggcgcg acggcgggga atggcggcgg
     2101 ctgggcacag ccggcaaggg cgagaagcag cagaccggcg aggaggcgac gatggtggcc
     2161 catggctcac gccagcccga gctcgcgctc gagcgcgccg cacagcatct ggcccagcat
     2221 gatgtgcatc tcctggatgc gcgcggtggt gcgcgagggg acgatgagca ggggatcggc
     2281 gacgcctttg agcaagccgc cttcaccgcc gctgagcgct gccggcacga tgcccatctc
     2341 gcgcgccgcc tcgagcgcca ggatgacgtt gcggctcttc cccgacgtgc tgatgccgag
     2401 ggcgacgtcg cccttggcgc ccaatgcctt gagctggcgc acgaagagct gctcgaatcc
     2461 gaggtcattg ccgatcgccg tcagcgccga agtgtcggtg gtgagcgcga cggcggcgat
     2521 gggcttgcgg tccttgatgt aacggacggt cagctcggtg gcgagatgct gggcgtcggc
     2581 cgcgctgccg ccattgccga agaacatgat cttgccgccg cgccggaccg ccgcgccgac
     2641 ggcgtcgagg aggcgcatga agggcgcctc gcattgcgcg agcgtcgcct tcaataccgt
     2701 ctcgtgctcg gcgaattcgc cgcggaagaa tgcctgccgg tccatgtcga tcgcttcctt
     2761 gtcacttcgc gacgaagaat cgccgccgcg ccggcgatag ccgttaccgc gcgggaatgt
     2821 caatcgccca gcgctcccaa tgcgccacga tctcgtcgac cagcggcctg ccggcaacga
     2881 cgagattggc gatcgagaca gagaaccccg ccgatatcgc cttcagcgac gcaaaagcgc
     2941 tctggccagg atagggccga tcgaaatcgg agccggtccg cagcacggcg acgcggcgga
     3001 tatccagcag ccgtgcaccg gcccctctct tcagcgcctc gaaggtcgca ttgtcctcct
     3061 gttgggtcgt gcaatatctg cccttgccgt cagtcagcag gcgggtccac tgctcggcac
     3121 gctcgcccag cttgctgcct tgccaccagg tgtcgccggc ggcggtgtcg cattggacca
     3181 ccgaaggcgc gtccgcggcc ggcagcttgc catagtgccg ccgataggcg cgcgcggcat
     3241 cgctgtcctg caacgtcatc ccgcgggaga gcgccacggc ccattgcagc agatcttcgt
     3301 tgagctgaaa cacctccgtc cggtattcga gcgtcggctt gacagtcggt cccgtggtcc
     3361 cgatgccgat atatccggag ggccaagcct tgggcatctc ccgcgcatcg atctcctgag
     3421 cgatgccgta gtcgaccagg taccgcgccc aggtcgcaga cccgagtgtg ccgatcttgg
     3481 gatcgatccc ggcgatgccg gcgacgagaa aatagctgtg ccggaagtcg aagcggccgc
     3541 tgaagacgag agcggtgatc gatgccgccg cgttcgcgtg gcccatgcct gtcgtcacct
     3601 ggcacacatc atcgccattg caccgaaccg tcgggtagtc gggcgacaac ccgggcacgg
     3661 cgatctcgtc cttgagcgcg agcggctccc gccacacctc gccctcgggt ccgaacatgc
     3721 tgatgatcag gaccttgacc ggccgcggcg atccgctcgc ggcagcgggc gttgtgaccg
     3781 tcggaggact cgcggcgcat gacgcgaggg cgagcagcgc cagcaacgga aagagtcgac
     3841 gaagaggctc cacggcggcg atctccctgc gcccgatgat agacgacttc ccgcgaaacc
     3901 ggcagatggc ccgtctcgac aggggcaatg ggtgacgttg catctgcttg cctccgccgc
     3961 gagcctggga taggcctagc gccatggcgc ttgccacccc cggccgccgc cgcgcgctga
     4021 tcctcgatcg cgacggcgtc atcaaccatg atctgggcta tgtccatcgc atcgaggatt
     4081 gccgcttcat cgacggcatc ttcgtcatgg ccaaggcctt tgccgatcgc ggcttcgcc
//
LOCUS       DASYNS010000220         7938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2390228, whole genome shotgun sequence.
ACCESSION   DASYNS010000220 DASYNS010000000
VERSION     DASYNS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390228"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VGP50_06880"
     CDS             <1..211
                     /locus_tag="VGP50_06880"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEV8027136.1"
                     /translation="RIFIGSLPRQFVMIGICGGYTTFSAFSLETLEILRSGRAAAAGA
                     NVVLSLALCLVAAAGGDAIARRLNA"
     gene            300..674
                     /gene="crcB"
                     /locus_tag="VGP50_06885"
     CDS             300..674
                     /gene="crcB"
                     /locus_tag="VGP50_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV8027137.1"
                     /translation="MDHLLVIIGSALGGMARYGLSGVVARRIGETFPWGTLVVNVSGC
                     FLIGVFAATVAATDHALRSLLMTGICGGYTTFSSFSLQTLTLARDGDWLRASFNIAAS
                     LVVCLAAVGAGFVLGGLSLKGG"
     gene            679..1020
                     /locus_tag="VGP50_06890"
     CDS             679..1020
                     /locus_tag="VGP50_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEV8027138.1"
                     /translation="MQVPRDAVLLRIFIGEDDRHQHMPLYEAIVLKAREQQLAGATVL
                     RGPMGFGHSSRLHTTKVLRLSEDLPVIVEVVDSEEKIEAFLKLLDGMMGSGLVTLEKV
                     KVLQYGEREGG"
     gene            1021..1854
                     /gene="mazG"
                     /locus_tag="VGP50_06895"
     CDS             1021..1854
                     /gene="mazG"
                     /locus_tag="VGP50_06895"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEV8027139.1"
                     /translation="MSDAPDLLRLLDIMARLRDPERGCPWDLAQSFATIAPYTVEEAY
                     EVAEACERGDMGALKDELGDLLFQVVFHARMAQEAGLFAFNDVARAIADKMLRRHPHV
                     FADAVIADAAAQTIAWEEHKAAERRAKAAANGGAPISALDGVGDALPALIRALKLQAR
                     AARVGFDWATPPPILDKIEEELGELRDAMDEGAAPEALEEEMGDLLFAVVNLARRLTI
                     DPEQALRHACRKFDRRFRRVEALLAESGRAPATATLDEMEEKWQEAKVEERSTGRSSN
                     D"
     gene            2272..2526
                     /locus_tag="VGP50_06900"
     CDS             2272..2526
                     /locus_tag="VGP50_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027140.1"
                     /translation="MLLTALAIFLVLGGAEALLFASAQAWLAHMLGPAGAVLATVLMI
                     ALPIANIFIAWFAARRIVAGVATKPKDKPIRRRIVGQHQF"
     gene            complement(2554..3102)
                     /locus_tag="VGP50_06905"
     CDS             complement(2554..3102)
                     /locus_tag="VGP50_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027141.1"
                     /translation="MMRPSVAAAAILSLLASPAFAGSDSSGKAVPAANAAAPATSSSS
                     TTSSSFDALSAGDRKIARALFLAQHPTANGPAPLSLNQIADLKTSSSWAKVFKQMQAG
                     GLVQAKSLGQVVEGYEHQRRTERRDSTSSSRQVLVTNGQGFIVASSRNRGAGALHGGD
                     EVAERGGTALQTAINVRSKDER"
     gene            complement(3102..3572)
                     /locus_tag="VGP50_06910"
     CDS             complement(3102..3572)
                     /locus_tag="VGP50_06910"
                     /inference="COORDINATES: protein motif:HMM:NF023514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HEV8027142.1"
                     /translation="MKIARGPVLLLCAAALQGCTAAAIGLTIATAGAGAGMSVGVEHT
                     LNGIVYKTFAASANDVRFATLKTLDQMGMPLTTDEATKTGWQLTATASGRTLDIQLER
                     LTDTATRMRVVANEGWIIFKDSATATEIILQTAQTLQDDAVAARAGETHRRKSS"
     gene            3885..4430
                     /locus_tag="VGP50_06915"
     CDS             3885..4430
                     /locus_tag="VGP50_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027143.1"
                     /translation="MALHWSRAAICAAGAAWLAACAGEPVSYYVPPPGLTPPQGVTIL
                     GSQGDTFFLQSIEYHAVWEIDGLPVEDPVYRWNKPLLVTANQPHRLRVVYDWGPVAGS
                     HAFDFTGAPGSTVVLEAEDVERQKLARMWLQDAQTGQLLTAKEDVPLDYIAIANIPVG
                     YSNTAIVNQVIRNGTTVPMMR"
     gene            complement(4603..5109)
                     /gene="coaD"
                     /locus_tag="VGP50_06920"
     CDS             complement(4603..5109)
                     /gene="coaD"
                     /locus_tag="VGP50_06920"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV8027144.1"
                     /translation="MSASRIGVYPGSFDPVTYGHTDIIFRGTKIVDRLIVGVGHNVGK
                     SPLFSIEERVAIVEDEIANVADAATRKRIQVKPFDNLLMHFAMEQGASVILRGLRAVS
                     DFEIEFQMAGMNNRLNASVETVFLMANDKYQFIASRFVKEIAQLGGDVTPFVSPRVAQ
                     RIAAKFGR"
     gene            complement(5124..7901)
                     /gene="gyrA"
                     /locus_tag="VGP50_06925"
     CDS             complement(5124..7901)
                     /gene="gyrA"
                     /locus_tag="VGP50_06925"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV8027145.1"
                     /translation="MTGSPASPPPFDITPVSIEEEMKRSYLDYAMSVIVARALPDVRD
                     GLKPVHRRILFVMKENGYDWNRPFRKSAAVVGDVMGQYHPHAGEAIYDAMVRMAQDFA
                     MRLPLIEGQGNYGSMDGDPPAAMRYTEARLARASDPLLSDIDKETVDFQPNYDGTRQE
                     PKVLPARFPNILVNGGSGIAVGMATNIPTHNLSEVIDGCLAFIDNPNVTTDELMELIP
                     GPDFPTGGLIMGRVGIQAAYKLGRGSVVVRGKTHIEELRKEREAIVVTEIPYQVNKAR
                     MVERIAECVREKLIEGIADLRDESDRDGVRVVVELKRDAVADVVLNQLYRHTPLQTSF
                     GVNMLALNGGRPELLTLRDIIAAFVAFREEVVTRRTTFLIARARERAHILLGLAVAVA
                     NIDAIIELIRKAPDPAAARAALLSRLWPAESVAPLIARIEEAGHGGTQADGGYRLSEE
                     QAKGILDLRLQRLTALERDKIAEELDGIAEEIKEYLQILGSRERLLEVLRQEFREVKE
                     QFATPRRTVIEDLEFEADIEALIQREDMVVTVTHGGYIKRVPLSTYRSQRRGGRGRSG
                     MATREEDFVSQVFIASTHAPVLFFTSTGHVYKLKAYRLPVGTPQARGKAMVNLLPNLA
                     PGENISTVLPLPEDEASWGNYTAVFATAKGHVRRNALSDFVNVNVTGKMAMKFEGDDA
                     DDRLIAVAICGPDHDVLLATRNGRSIRFPANDVRVFTGRSSVGVRGVKLAAGDAVISL
                     SILRHEEVDTPVRAAYLRLASQRRRGAGEEGDAAEAAEPEEGEENGTAEASISEEQFA
                     DLASREEFLLSVTEKGFGVRSSAYDYRITGRSGQGIENMNLARRKDAVVAAFPVGARD
                     QIIVITNTGMVMRMPVEGVSIKRRRSQGVVVFKVGEGERVVSVAHLAEAGENGAGDED
                     EGETAAPEAPP"
BASE COUNT         1315 a         2590 c         2600 g         1433 t
ORIGIN      
        1 ccgcatcttc atcggcagcc tgccccgcca attcgtgatg atcggcatct gcggcggcta
       61 taccaccttc tcggccttca gcctggagac gctcgagatc ttgcgcagcg gccgcgcggc
      121 ggcggccggg gccaatgtag tcctgtcgct ggcgctctgc ctggtcgccg ccgcgggcgg
      181 cgatgcgatc gcccgccggc ttaatgcatg accggaaata tggcggttat cccattgttc
      241 ctgcgatatt cattgcggcg cagcagaaat ctttgctgcg ccgcggaagg gagcctagga
      301 tggatcatct gctggtgatc atcggcagcg ctctcggcgg catggcgcgc tacgggcttt
      361 cgggtgtggt ggcgcgccgc atcggcgaga cctttccctg gggcacgctg gtggtgaacg
      421 tgtccggctg tttcctgatc ggcgtcttcg ccgcgaccgt cgccgcgacg gaccacgcgc
      481 tgcgcagcct gctcatgacc gggatctgcg gcggctacac caccttctcg tctttcagcc
      541 tgcagacgct gaccctggcg cgggacggcg attggctgcg cgccagcttc aacattgccg
      601 cttctctcgt cgtctgtctt gccgccgtgg gggccggctt cgtcctcggc gggttgtcgc
      661 tcaaaggagg atgatctcat gcaggtccca cgcgacgcgg tgctgctgcg gatcttcatc
      721 ggcgaggacg atcgccatca gcacatgccg ctctacgagg cgatcgtgct gaaggcccgc
      781 gagcagcagc tggcaggcgc cacggtgctg cgcggcccca tgggcttcgg ccattcgagc
      841 cggctgcata cgacgaaggt gctccgcctc tcggaagatc tcccggtcat cgtcgaggtc
      901 gtcgacagcg aggagaagat cgaggccttc ctcaaattgc tcgacggcat gatggggagc
      961 ggcctcgtca ccctcgagaa ggtgaaggtg ctgcaatatg gcgaacgcga gggcggttga
     1021 atgagtgacg ccccggatct gctgcgcctc ctcgatatca tggcgcggct gcgcgatccc
     1081 gagcgcggct gcccctggga tctggcgcaa agcttcgcca ccatcgcccc gtataccgtc
     1141 gaagaagctt atgaggtggc cgaagcgtgc gagcgcggcg acatgggagc gctcaaggac
     1201 gaactgggcg acctcctgtt ccaggtcgtc ttccatgcgc gcatggcgca agaggccggg
     1261 cttttcgctt tcaacgacgt cgctcgcgcc atcgccgaca agatgctgcg tcggcatccc
     1321 catgtgttcg ccgacgccgt catcgccgat gccgccgcgc agacgatcgc ctgggaggag
     1381 cacaaggcag cagagcgccg ggccaaagcg gccgcgaatg gcggagcgcc cataagcgcg
     1441 ctcgacggtg tcggcgatgc gctgccggcg ctcatccgcg cgctcaagct gcaggcgcgc
     1501 gccgcccggg tcggcttcga ctgggcgacg ccgccgccga tcctcgacaa gatcgaagag
     1561 gagctgggcg agctgcgcga cgccatggac gagggtgctg cgccggaggc actcgaggag
     1621 gagatgggcg atctgctctt cgccgtcgtg aacctggcgc ggcggctcac gatcgatccc
     1681 gagcaggcgc tgcgccatgc gtgccgcaaa ttcgaccgcc gcttccgccg cgtcgaagcg
     1741 ttgcttgcgg aatccggccg cgcgcccgcg accgccacgc tcgatgagat ggaagaaaag
     1801 tggcaagaag caaaggtgga agagcgcagc accggccgct ccagcaatga ttaaattttc
     1861 ccgtgcaatc gcctaaaatg ctcgacaaaa atcctgtaaa ttaacactct gtttgcgcat
     1921 aatcctcggt gaaagttctg gtaactattc gcgagttgcc acctcctggg gataattctt
     1981 tgagcaaaga cagaccgatt gccgctttgt cttcgatatt cctggagaac ccatcgcgcg
     2041 ggcgcgccgc ctccgtggtc ttgagcgaca cgaaaccggc agcgcccgcc gatgcggttt
     2101 tcttcaataa aggatgggcg accgcggcgg ggttccgcct gcccgcgcgg tccggcgcca
     2161 cgtcccagcc gcggcccacc gcctcgacgc atgagatcca gctcttccgc cttttgaagc
     2221 acgtagcacc caacacggcc aaggcccatt aggcccatca gggagaccgc gatgctattg
     2281 acagctctgg cgatttttct agttcttggc ggcgcggaag cgctgctctt cgcgagcgcg
     2341 caggcctggc ttgctcacat gctcggcccc gccggcgcag tgctggcgac ggtgctcatg
     2401 atcgccttgc cgatcgccaa catcttcatc gcgtggtttg ccgcgcggcg catcgtcgcc
     2461 ggcgtcgcga ccaagccgaa agacaagccg atccgccgcc gcatcgtcgg tcagcatcaa
     2521 ttctgagatg aaacgccggt ccgatccgaa ggattagcgc tcgtccttgg accgcacatt
     2581 gatggccgtc tgaagcgctg tgccgccgcg ctcggcaacc tcatcaccgc catgcagagc
     2641 gccggcgccg cggttgcgcg acgaagcgac gatgaagccc tggccgttgg tgacgagaac
     2701 ctgccgcgat gacgatgtgc tgtcgcggcg ctcggtacgc cgctgatgct catagccttc
     2761 gaccacctgg cccaggctct tcgcctggac gagcccaccc gcctgcatct gcttgaagac
     2821 tttcgcccag ctgctgctgg tcttgaggtc ggcgatctgg ttgaggctga gcggcgcggg
     2881 cccgttcgcc gtcggatgct gcgccaggaa cagcgcgcgg gcgatcttgc ggtcgccggc
     2941 agagagcgca tcaaagctgc tcgacgtcgt agagctgctc gacgtcgcag gcgcggccgc
     3001 gttggcagca ggcaccgcct tgccgctgga atcggaaccc gcgaaagcgg ggctcgccag
     3061 cagcgacaga atcgctgccg cggcaacgct cgggcgcatc attacgatga cttccggcga
     3121 tgcgtctcgc cggcgcgcgc ggcgacggcg tcatcctgaa gggtctgcgc cgtttgcagg
     3181 atgatctcgg tcgcggtcgc gctgtccttg aagataatcc atccctcgtt ggcaacgacg
     3241 cgcatacgcg tcgccgtgtc ggtgaggcgc tcgagctgga tgtcgagcgt gcgaccgctc
     3301 gcggtggcgg tgagctgcca gcccgtcttg gtggcctcgt ccgtggtcaa gggcatgccc
     3361 atctgatcga gcgtcttcag cgtggcaaag cgcacgtcat tggccgaagc ggcgaaggtc
     3421 ttgtagacga tgccgttcaa cgtgtgctcg acgccgacgc tcatgcccgc gccggcgccc
     3481 gccgtggcga tggtcaggcc gatggcagcg gccgtgcagc cctgaagcgc tgcggcacag
     3541 agcaggagaa ccggaccgcg cgcgattttc ataaaattgt tcattgcagc caccctgaat
     3601 tgaatgtcaa agctaggcgt gtggcacaaa ggaggcaaac ggcatcgccg aaaacttgag
     3661 acgactgcaa gtgaagatgt ccgcatgaat agcggctagt tcgttgtaat cgccgaagca
     3721 atgggtcgcc ctcgacggct ggctgcgcct gctttgcgaa ccggcatcat cgacaattgt
     3781 cacgacaatg ccggcgcgcc cggaccgtca gtgacagcgg cgtgatttgc cgccctttcc
     3841 aatgggtgtt aggcttttat caaggctttc tctggaggtg tcccatggct cttcactggt
     3901 cgcgagcggc gatttgtgcg gcgggcgcgg cgtggctcgc cgcctgcgcc ggcgagcccg
     3961 tcagctatta tgtgccgccg cctggcctca cgccgccgca aggcgtgacc attctcggca
     4021 gtcaaggcga cacgttcttc ctgcaatcga tcgagtatca cgcggtgtgg gagatcgacg
     4081 gcctgcccgt cgaggatccc gtctatcgct ggaacaagcc gctgctcgtc acggccaacc
     4141 agccgcatcg cttgcgcgtc gtctatgact ggggtccggt cgccggcagc cacgctttcg
     4201 atttcacggg cgccccgggc agcacggtcg tgctcgaggc cgaggacgtc gagcgccaaa
     4261 agctagcgcg gatgtggctc caggatgcgc agaccgggca gctgctcacg gccaaggagg
     4321 atgtgccgct cgattacatc gcgatagcga acatcccggt gggctactcg aatacggcca
     4381 tcgtgaacca ggtcatccgc aacgggacga ccgtgccgat gatgcgttga gggcgttgtt
     4441 cgggaggcac tagcagagcg gagaaatctc gctgcatttc gggcgtgcgt tcttcgacaa
     4501 gctcaggatg aggaagatcg ggcgcggcat ctggcagatg cccgactccc cccttcctca
     4561 tcctgagctt gtcgaagaac gcaccggcag cattgcagcg gctcaccgcc cgaacttcgc
     4621 ggcgatgcgt tgcgcgacgc gggggctcac gaagggggtg acgtcgccgc cgagctgggc
     4681 gatctccttg acgaaacgcg aggcgatgaa ctgatacttg tcgttggcca tgaggaagac
     4741 cgtctcgacg ctggcgttga ggcggttgtt catgccggcc atctggaact cgatctcgaa
     4801 atccgagacc gcgcgcaggc cgcgcaagat gacggaggcg ccctgctcca tggcgaaatg
     4861 catcagcaga ttgtcgaacg gcttgacctg gatgcgcttt ctcgtcgcgg cgtccgcgac
     4921 attcgcgatc tcgtcctcga cgatcgcgac gcgctcctcg atcgagaaga gcgggctttt
     4981 gccgacgttg tggccgacgc cgacgatcag ccggtcgacg atcttcgtgc ctctgaagat
     5041 gatgtcggta tggccgtaag tcaccggatc gaaggaaccg ggatagacgc cgatgcgcga
     5101 cgcgctcatg ttcatgcccc ctcttacggc ggcgcttccg gcgcggccgt ctcgccctcg
     5161 tcctcatccc ctgcgccgtt ctctccggcc tcggcgagat gcgccaccga gaccacgcgc
     5221 tccccctccc cgactttgaa cacgacgacg ccctggctgc gccggcgctt gatgctgacg
     5281 ccctcgactg gcatgcgcat caccatgccg gtgttggtga tcacgatgat ctggtcgcgc
     5341 gctcccacgg ggaaggcggc gaccacggcg tccttgcgcc gcgccagatt catgttctcg
     5401 atgccctgcc cgctgcggcc ggtgatgcga taatcatagg ccgagctgcg cacgccaaag
     5461 cccttctcgg tgacgctgag caggaactcc tcgcgcgacg cgagatcggc gaactgctcc
     5521 tccgagatcg atgcctcggc cgtgccgttc tcctcgcctt cctcgggctc ggcggcctcg
     5581 gcggcgtcgc cttcctcgcc cgcgccgcgc cggcgctgcg acgccaggcg gagataggcg
     5641 gcacgcaccg gggtgtcgac ctcttcatgg cgcaggatcg aaagcgagat cacggcatcg
     5701 cccgcggcaa gcttgacgcc gcgcacgccc acggagctgc ggccggtgaa gacgcgcacg
     5761 tcgttcgccg ggaagcggat cgagcggccg ttgcgcgtcg cgagcagcac gtcgtgatcc
     5821 gggccgcaga tggccacggc aatgagccga tcgtcggcgt cgtcgccttc gaacttcatc
     5881 gccatcttgc cggtgacgtt gacgttgacg aaatccgaga gcgcgtttcg ccgcacatga
     5941 cctttggcgg tggcgaagac cgccgtatag ttgccccagg acgcttcgtc ctcgggcagc
     6001 ggcagcacgg tcgagatgtt ctcgcccggc gccagattgg gcagcagatt gaccatcgcc
     6061 ttgccgcgcg cctgcggcgt gcccaccggc aggcgataag ccttgagctt gtagacgtga
     6121 cccgtcgagg tgaagaacag caccggcgca tgggtcgagg cgatgaagac ctggctgacg
     6181 aaatcctctt cgcgcgtcgc cattcccgag cgcccgcgcc cgccgcggcg ctgcgagcga
     6241 taggtcgaga gcggcacgcg cttgatgtag ccgccatggg tcaccgtcac caccatatcc
     6301 tcgcgctgga tcagcgcttc gatatcggcc tcgaattcga gatcctcgat gacggtgcgg
     6361 cgcggcgtcg cgaactgctc tttgacctcg cggaattcct ggcgcagcac ctcgagaagc
     6421 cgctcgcgcg agcccaggat ctggagatac tccttgatct cctcggcgat gccgtcgagc
     6481 tcctcggcga tcttgtcgcg ctcgagcgcc gtcagccgct gcaggcgaag atcgaggatg
     6541 cctttcgcct gctcttcgga gagacggtag ccgccgtcgg cctgcgtgcc gccatgcccc
     6601 gcttcctcga tgcgcgcgat gagcggcgcg acgctctccg ccggccagag ccggctcagg
     6661 agcgccgcgc gtgccgcggc cggatcgggc gccttgcgga tgagctcgat gatcgcatcg
     6721 atattcgcca ccgccacggc gaggccgagg agaatatgcg cgcgctcgcg cgccctggcg
     6781 atgaggaagg tggtccggcg ggtgacgacc tcctcgcgaa aggcgacgaa agcggcgatg
     6841 atgtcgcgca acgtcaggag ctcgggacgg ccgccattca acgccagcat gttgacgccg
     6901 aagctcgtct gcagcggcgt gtggcgatag agctggttca gcaccacatc ggcgacggcg
     6961 tcgcgcttca gctcgacgac gacccggacg ccgtcgcgat cactctcgtc gcgcagatcg
     7021 gcgatgccct cgatgagctt ctcgcgcacg cattcggcga tgcgctccac catccgcgcc
     7081 ttgttgacct ggtacggaat ctcggtgacg acgatcgcct cgcgctcctt gcgcagctcc
     7141 tcgatgtgcg tcttgccgcg caccaccacc gagccacggc cgagcttgta ggcggcctgg
     7201 atgccgacac gccccatgat gagcccgccg gtcgggaagt cagggccggg aatgagctcc
     7261 atcagctcgt cggtggtgac gttgggattg tcgatgaaag cgaggcagcc gtcgatcact
     7321 tccgacagat tgtgcgtcgg gatgttggtc gccatgccga cggcgatgcc gctgccgccg
     7381 ttgacgagga tgttcgggaa gcgtgccggc agcactttcg gttcttggcg cgtgccgtca
     7441 taattcggct ggaagtcgac ggtctccttg tcgatgtcgc tgagcagcgg gtcggaggcg
     7501 cgcgccagcc gtgcctcggt gtagcgcatc gccgccggcg gatcgccgtc catcgagccg
     7561 taattgccct gcccttcgat cagcggcagg cgcatggcga agtcctgcgc catgcggacc
     7621 atggcatcgt agatcgcttc gccggcatgc ggatggtatt gccccatcac gtcgccgacc
     7681 accgccgccg atttgcggaa cggccggttc cagtcgtagc cgttctcctt catgacgaag
     7741 agaatgcggc gatgcaccgg cttcaatccg tcgcgcacat cgggcagcgc gcgcgccacg
     7801 atcacgctca tggcgtaatc gagatacgac cgcttcatct cctcttcgat ggagaccggc
     7861 gtgatgtcga agggcggagg tgaagcggga gagccggtca agggacgaat ccagatactg
     7921 caaacgccgg ctgggggc
//
LOCUS       DASYNS010000221         3953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1766173, whole genome shotgun sequence.
ACCESSION   DASYNS010000221 DASYNS010000000
VERSION     DASYNS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3953
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1766173"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VGP50_06930"
     CDS             complement(<1..657)
                     /locus_tag="VGP50_06930"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV8027146.1"
                     /translation="MSGAAAPPVPTLLLVGCGKMGSALLSGWLAQDLAQRYLVVEPGR
                     VPDTLRARPEVAILPTPDQLPRDASPGVVVLAVKPQAMAAVLPAYRRFAAVGTLFLSI
                     AAGKTLSFFARALGAAAVVRAMPNTPAAIGRGITVACASAAVAAPQRALAERLLGAVG
                     EVAWVADEALLDPVTAVSGSGPAYVFLLIECLAKAGVAAGLPEALATQLARATVAGSA
                     A"
     gene            complement(654..1160)
                     /locus_tag="VGP50_06935"
     CDS             complement(654..1160)
                     /locus_tag="VGP50_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEV8027147.1"
                     /translation="MTTSLSLETPVATANPIDLVEEIVQANEWAHDRASDEEMVVEIA
                     GRWCSYQLLFVWQREINALHFSCGFAMKVPKARRAAVFELLAVVNERLWLGHFDLASD
                     DQSPAFRQGVLLRGASGASVEQIEDLVDISVSECERFYPAFQLVVWGGKPAEEAIAAA
                     MIDPLGEA"
     gene            complement(1300..1635)
                     /locus_tag="VGP50_06940"
     CDS             complement(1300..1635)
                     /locus_tag="VGP50_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEV8027148.1"
                     /translation="MQTENRLFDDLARVATGALGALTGVREEIEARMREQFERILDRM
                     NLVRREEFDAVQAMAQKARAAQEALEQRVAALEARLAQQPPSEEAAAAPLRSRPATKR
                     ANKSPPDGE"
     gene            1865..2851
                     /gene="rfaD"
                     /locus_tag="VGP50_06945"
     CDS             1865..2851
                     /gene="rfaD"
                     /locus_tag="VGP50_06945"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEV8027149.1"
                     /translation="MIVITGGAGFIGSNLAASLEARGESDIVICDRLGHEEKWRNIAK
                     RELAAVIPPEALLDFLDGHSREIGVLFHMGAISSTTEADVDLVAATNFSLSLRLWEWC
                     TESETRFIYASSAATYGDGAAGFDDDGSVAALARLKPLNAYGWSKHLFDRRIARLLER
                     HAARPPQWVGLKFFNVYGPNEYHKGGMKSVVAQLCPRAQKGEAARLFRSDRPDVADGG
                     QKRDFVHVRDCAEMMLWLLDHPAVNGVFNIGSGRARSFADLAAAVYAALGREAHIDYV
                     DTPVEIRDKYQYFTEARMERLRAAGYDRPATPLEDGVRDYVQHYLAAPDPYR"
     gene            2871..3713
                     /gene="lgt"
                     /locus_tag="VGP50_06950"
     CDS             2871..3713
                     /gene="lgt"
                     /locus_tag="VGP50_06950"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV8027150.1"
                     /translation="MLTIPYPDISPNIFTLPPFTIGQLTLGPFALRWYALAYIAGLII
                     GWRYCLALARKPPQVTAPQDVDDFLVWATIGVVLGGRLGYVLFYNLPEYLQHPIEILY
                     VWRGGMSFHGGAIGVCLAIFLFCRQRHIPLLGFADIIACAAPIGLLFGRIANFINAEL
                     WGRPTDVPWAMVFPTDPTAQPRHPSQLYEACLEGLVLFLLISALERFAGARQRPGLLT
                     GVFLMGYAIARIIVEFFRQPDAQLGFLWLGATMGQLLSLPLFLIGLFLVLRALRTSHT
                     PATA"
     gene            3710..>3953
                     /locus_tag="VGP50_06955"
     CDS             3710..>3953
                     /locus_tag="VGP50_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8027151.1"
                     /translation="MNALERHLIARIRADGPLSIADYMAEALGHPLHGYYTTRDPLGA
                     RGDFITAPEVSQVFGELIGFWCAAGWERMGRPDPVIL"
BASE COUNT          576 a         1477 c         1269 g          631 t
ORIGIN      
        1 cgccgccgac cccgcgaccg tcgcgcgcgc gagctgcgtg gcgagcgcct cgggcagacc
       61 cgccgccacc cccgctttcg ccaggcattc gatcagcagg aacacatagg cgggcccgct
      121 ccccgacacc gccgtcaccg ggtcgagcag cgcttcgtcc gccacccacg ccacctcgcc
      181 caccgccccc aggaggcgct cggcgagcgc ccgctgcggt gctgcgaccg cggcgctggc
      241 gcaggccacg gtgatgccgc ggccgatcgc cgcgggcgtg ttgggcatgg cccgcacgac
      301 cgccgccgcc cccagcgcgc gcgcgaaaaa gctcagcgtc ttcccggcgg cgatcgagag
      361 gaacagcgtt cccacggcgg cgaagcgccg ataggccggc agcaccgccg ccatggcctg
      421 cggcttgacc gcgagcacga cgacgccggg cgaggcatcg cgcggcagtt gatcgggcgt
      481 ggggaggatc gcgacctccg gccgcgcacg cagcgtgtcg ggcacacgac cgggctcgac
      541 gacgagatag cgctgcgcca gatcttgcgc gagccagccg ctcagcagcg cgcttcccat
      601 cttgccgcag ccgaccagga gaagcgtcgg gaccggagga gccgcggctc cgctcacgcc
      661 tcgcccaagg gatcgatcat cgccgccgcg atcgcctcct cggccggctt gccgccccag
      721 acgacgagct ggaaggccgg gtagaagcgc tcgcactcgc tcacggagat gtcgacgaga
      781 tcctcgatct gctcgacgct ggcgccgctc gcgccgcgca gcagcacgcc ctggcggaag
      841 gccggcgact ggtcgtccga ggcgagatcg aaatgtccga gccagagccg ctcattgacc
      901 accgcgagca gctcgaagac ggcggcgcgc cgcgctttcg gcaccttcat ggcgaagccg
      961 caggagaaat ggagcgcgtt gatctcgcgt tgccagacga agagcagctg gtaggagcac
     1021 cagcgtccgg cgatctcgac caccatctcc tcgtcgctgg cgcggtcatg ggcccattca
     1081 ttggcctgga cgatttcctc gaccaggtcg atggggttgg cggtggcgac tggggtttcg
     1141 agcgatagcg aggtcgtcat cgcgccctcc tgcgagccgt cgacccgggg gaactgcgat
     1201 atccgttgtg gacgcccccc aacgcccaca caagatgtag cctgccaaac gccgactccg
     1261 gatgcaagcg ccgcgtttgc ctcagccctt gacgctgcct cattcgccgt cgggcggcga
     1321 cttgttggcg cgcttcgtcg ccgggcgcga gcgcagcggc gctgcggccg cttcctcgct
     1381 cggcggctgc tgggcgagcc gcgcctcgag tgcggcgacg cgctgctcga gcgcctcttg
     1441 cgccgcgcgc gctttctgcg ccatcgcctg cacggcgtcg aactcctcgc gccgcaccag
     1501 gttcatgcgg tcgaggatgc gctcgaactg ctcgcgcatc ctcgcctcga tctcctcgcg
     1561 caccccggta agcgcgccca aagcgccggt ggcgacccgg gcgaggtcat cgaagaggcg
     1621 gttctcggtc tgcatggcgt gcgccctcct ttagccgcaa cgggctagca tagaatatgg
     1681 gtcgccgcca aaccgatttc gattgccggc cggccgccag cccctataac acgccggcga
     1741 tgagcggatc ggcacaactg ggtcggacgc gcgacgaatt cccgctcccc ttgggggaga
     1801 gggaggggcc cgccgcgcgg cggcgggagg gtaggggcgc ggccgcaatg ccggggacgt
     1861 cccgatgatc gtcatcaccg gcggcgcggg cttcatcggc tcgaacctgg ccgcttcgct
     1921 cgaggcgcgg ggcgaaagcg acatcgtcat ttgcgaccgc ctcggccacg aggagaaatg
     1981 gcgcaatatc gccaagcgcg agctcgccgc ggtcatcccg cccgaagcgc tcctcgactt
     2041 cctcgatggc catagccgcg agatcggcgt cctcttccac atgggcgcga tctcctccac
     2101 caccgaggcc gatgtcgacc tcgtcgccgc caccaatttc tcgctctcgc tccggctctg
     2161 ggaatggtgc accgagagcg agacgcggtt catctacgcc tcctcggcgg cgacctatgg
     2221 cgacggcgcg gcgggcttcg acgacgacgg ctcggttgcg gcgctggcgc gcttgaagcc
     2281 gctcaacgcc tatggctggt cgaagcatct cttcgaccgt cgcatcgcgc ggctcctcga
     2341 gcgccacgcc gctcggccgc cgcaatgggt cggactcaaa ttcttcaacg tctacggccc
     2401 caacgaatac cacaagggcg ggatgaagag cgtcgtggcg caactctgtc cgcgcgccca
     2461 gaaaggcgag gccgcgcggc tcttccgctc ggatcgtccg gacgtggccg atggcgggca
     2521 gaagcgcgac ttcgtccatg tgcgcgactg cgccgagatg atgctctggc tcttggatca
     2581 tccggccgtc aacggcgtct tcaacatcgg cagcggccgc gcgcgcagct tcgccgatct
     2641 cgccgccgcg gtctatgccg cgctcggccg cgaggcgcac atcgactacg tcgacacgcc
     2701 ggtcgagatc cgcgacaaat accagtactt caccgaggcc cgcatggaac ggctgcgcgc
     2761 cgccggctac gatcggcccg cgacgccgct cgaggacggc gtgcgcgact acgtgcagca
     2821 ttacctcgcc gcgcccgatc cctaccgctg aagcgcaaag cccgaggccc atgctcacca
     2881 tcccctatcc cgacatcagt cccaacatct tcacgctgcc gcccttcacc atcgggcagc
     2941 tgacgctcgg ccctttcgcg ctgcgctggt atgcgctcgc ctacatcgcc ggcctcatca
     3001 tcggctggcg ctactgcctc gctttggcgc ggaaaccgcc gcaggtgacg gcgccgcagg
     3061 acgtcgacga cttcctcgtc tgggcgacga tcggcgtcgt cctcggcggc cgcctcggct
     3121 atgtgctctt ctacaatctg cccgaatacc tgcagcatcc gatcgagatc ctctatgtct
     3181 ggcgcggcgg catgtccttt catggcggcg cgatcggcgt ctgcctggcg atctttctct
     3241 tctgccgcca gcgccacatt ccgcttctgg gctttgccga catcatcgcc tgcgctgcgc
     3301 ccatcggcct cttgttcggc cgcatcgcca atttcatcaa tgccgagctc tggggccgtc
     3361 ccaccgacgt gccttgggcc atggtcttcc cgaccgaccc gacggcccag ccgcgccatc
     3421 cgagccagct ctacgaagcc tgcctcgaag gcctcgtgct cttcctgctg atctcagcgc
     3481 tcgagcgttt cgccggcgcg cgccagcggc cgggcctgct gacgggcgtg ttcctcatgg
     3541 gctatgcgat cgcccgcatc atcgtcgagt tcttccgcca gcctgacgcg cagctgggat
     3601 ttctctggct cggcgccacc atgggccagc tcctgtcgct gccgctcttc cttatcggcc
     3661 tctttctggt gctgcgcgcg ctccgcacca gccacacgcc cgccacggcg tgaacgcgct
     3721 cgagcggcac ctgatcgcgc gcatccgcgc cgacggcccg ctcagcatcg ccgactacat
     3781 ggccgaggcg ctcggccatc cgctccacgg ctattacacg acccgcgacc ccctgggcgc
     3841 gcgcggcgac ttcatcaccg cgcccgaggt gagccaggtc ttcggcgagc tcatcggctt
     3901 ctggtgcgcc gcgggctggg agcgcatggg ccgccccgat cccgtgatcc tcg
//
LOCUS       DASYNS010000222         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5353082, whole genome shotgun sequence.
ACCESSION   DASYNS010000222 DASYNS010000000
VERSION     DASYNS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5353082"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VGP50_06960"
     CDS             complement(<1..561)
                     /locus_tag="VGP50_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV8027152.1"
                     /translation="MSSLPADARNADQIAHWNGPTGLRWVDHQEHQDALLRPILDAAL
                     ARAGVTPGERVVDIGCGCGGSTLELGRRVGRAGRVLGIDVSEPMLARAAERSTPELPV
                     AFVRADATVYPLPGDFDLLFSRFGVMFFAEPARAFANLRAGLRPGGRLLFVCFRTPRE
                     NPWMIVPLEAAYAHVPKPPETAPDDPG"
     gene            710..>2202
                     /locus_tag="VGP50_06965"
     CDS             710..>2202
                     /locus_tag="VGP50_06965"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEV8027153.1"
                     /translation="MGRRVLKIIGWIVAVLCVMVMASGVALWFGGGPAVAWVLRHPVS
                     GAIGRDIAVGRIEVDWGSWGRPTRLVAEDIHVANAPWGSAPDMFAAKRVEIALYAKTL
                     LLAPTRLPLIALDGAALLLETSKVGQGNWDFGLSSAAPKKRRQFPDLRRFTVTGSELT
                     FHNGETGAHSVLDLAKLDFSERDPREPVVIAIDGAFQKAPLHLRATVGPIAELRDPEK
                     PYPVKFDGTIDGLHLVADGTLAEPLDVAGVDGRFSLEGAKLADLAMRLGVPLPELPDV
                     RGTAELSGGNGRWTLKALTLRTGKSDLEGGIDIDTTGKVPALEANLTSSFIDMADFKG
                     LYGGSPARSSAPAKPADKSDRELPDTPIAIHKLPGLNARLNFEGSKIEATGGLPIERI
                     VLGLALRDGELDVRPLRLHTADGDVDLNFHFTPYTGPPHLNADLEIRHIDLHKLLGRP
                     SMPAMVQQTAGVAGGFAKIDTTGVSLRQFLGHMNGDAGVFLGNGQVSA"
BASE COUNT          354 a          786 c          748 g          314 t
ORIGIN      
        1 accggggtcg tcgggcgccg tctccggcgg cttcggcaca tgcgcataag ccgcttcgag
       61 cggcacgatc atccacgggt tctcgcgcgg cgtgcggaag cagacgaaga gcagccggcc
      121 gcccggacga agcccggcgc gcagattggc gaaggcgcgt gccggctcgg caaagaacat
      181 gacgccgaaa cgcgagaaga gcagatcgaa gtcgcccggc agcggataga cggtcgcgtc
      241 ggcgcggacg aacgcgacgg gcagctccgg cgtcgagcgt tctgcggcgc gcgccagcat
      301 cggctccgag acgtcgatgc cgagcacgcg gccggcacgg ccgacgcgcc ggccgagctc
      361 cagcgtcgag ccgccgcagc cacagccgat atcgacgacg cgctcgcccg gcgtcacgcc
      421 ggcccgcgcc agggccgcgt cgaggatcgg gcgcagcagc gcgtcctgat gctcctgatg
      481 atcgacccag cgcagaccgg tgggtccgtt ccagtgggcg atctgatcgg cgttccgcgc
      541 atcggcgggc aggctgctca tggcgggcgc gctcctgtgg gtcggatcac gtatagcaat
      601 cgccgtcgcc gctcccaagg gcaccgcagc gcgctccctg ctcgcggcca ggaacggcgg
      661 ccggtcaagg gtcgtttcct cgtgaagaag caagcgggga gatgcgagcg tgggccggcg
      721 cgtgctcaag atcatcggct ggatcgtcgc cgtcctgtgc gtcatggtga tggcgtcggg
      781 cgtcgcactc tggtttggcg gcggtcctgc cgtcgcctgg gtgctccgac atcccgtgag
      841 cggcgccatc ggccgcgaca tcgcggtcgg ccggatcgag gtcgattggg ggagctgggg
      901 caggccgacg cgcctcgtcg ccgaagacat ccatgtcgcc aacgcgccct ggggcagcgc
      961 gcccgacatg ttcgccgcga agcgcgtcga gatcgccctc tatgcgaaga ccctgctgct
     1021 cgcgccgacg cgcctcccgc tgatcgcgct cgatggcgcg gcgctgctgc tcgagacctc
     1081 gaaggttggc cagggcaact gggatttcgg gctgagctcg gcggcgccca agaagcggcg
     1141 ccaattcccc gacctgcggc gcttcaccgt caccgggtcc gagctcacat tccacaatgg
     1201 cgaaacgggc gcgcattcag tgctcgacct cgccaagctc gacttcagcg agcgcgatcc
     1261 gcgcgagccc gtcgtcatcg ccatcgacgg cgccttccag aaggcgccgc ttcatctccg
     1321 cgcgacggtc gggccgatcg ccgagctgcg cgatcccgag aagccctatc ccgtgaagtt
     1381 cgacggaacg atcgacgggc tgcatctcgt cgccgacggc accctcgctg agccgctcga
     1441 cgtcgccggc gtcgatggac ggttctcgct cgaaggcgcc aagctcgccg acctcgccat
     1501 gcgcctcggc gtgccgctgc cggagctgcc ggatgtccgg ggcacggccg agctcagcgg
     1561 cgggaacggg cgctggacgc tcaaagccct gacgctgcgc accggcaaga gcgatctcga
     1621 aggcggcatc gacatcgaca ccaccggcaa ggtccccgcg ctcgaggcga acctcacctc
     1681 gagcttcatc gacatggccg atttcaaggg cctttatggc ggcagtcctg cccggtcctc
     1741 ggcgccggcg aagcccgccg acaagagcga ccgcgagcta ccggacacgc ccatcgccat
     1801 ccacaagctg cccggactca acgccaggct gaatttcgag ggcagcaaga tcgaggcgac
     1861 gggcggcctt ccgatcgagc gtatcgtgct cggcctcgcg cttagagacg gcgagctcga
     1921 cgtcaggccg ctgcgccttc acaccgccga tggcgatgtc gatctgaact tccacttcac
     1981 gccctacacc ggcccgccgc acctcaatgc cgacctcgag atccgccaca tcgacctgca
     2041 caagctgctc ggccgtccaa gcatgccggc gatggtgcag cagaccgcag gcgtcgccgg
     2101 cggcttcgcc aagatcgaca cgacgggcgt gtcgctgcgt cagttcctcg gccatatgaa
     2161 cggcgatgcc ggcgtcttcc tcggcaacgg ccaggtcagc gc
//
LOCUS       DASYNS010000223         5821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3795010, whole genome shotgun sequence.
ACCESSION   DASYNS010000223 DASYNS010000000
VERSION     DASYNS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3795010"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VGP50_06970"
     CDS             complement(<1..185)
                     /locus_tag="VGP50_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027154.1"
                     /translation="MATAASRAAGKPPGLYRTLWHYAAGDRRRLVVFMTMLILAQLVK
                     LAIPYLSGAAVDAMQGGR"
     gene            348..1148
                     /locus_tag="VGP50_06975"
     CDS             348..1148
                     /locus_tag="VGP50_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEV8027155.1"
                     /translation="MLDPEKIAALIRETAQAEILPRFRMLGESDIREKKPGDFVTAAD
                     LAAERRMTEWLTEAAPGSLVLGEEAAAEDPGRLALLASEARVWIIDPVDGTANFARGE
                     PGFAVILAEVQKGEVCAGWIYDPLSDVMVTAEAGGGAWSAGRRLTVARDVAPAELVGG
                     AYGRTKAGVPAAKALEASGRIGRIRNRGCGGLEYLDLALGRAHFSLHSRSLPWDHAAG
                     MLITAEAGGVGAFLDGSRYDARIIDRPVLAAASAEAWQIVQEVVSALA"
     gene            1314..2093
                     /locus_tag="VGP50_06980"
     CDS             1314..2093
                     /locus_tag="VGP50_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027156.1"
                     /translation="MAWLAALGWLLPASAMAQTVPGWSIGPEVYWYEYRETPSFVYQW
                     GPFGGVDTSYTFKFNNVFATLDGDVDVGYLDYKASGSGRLNGIWNYKGDVRALFGVDL
                     GVSPSFYISPFAGAGYRVLFESQGGRATTIGQVAYDRLSQYYYLPLGIATSITAGSWV
                     LRPTAEYDLFIGGNQTSYLSQVGFNEDLNNRQTVGYGARASLLADTRTPWGRIAIGPF
                     FRYWNIGSSRQVPGFIGNNAAVFFEPPNNTIEAGLALHFLF"
     gene            2165..3094
                     /locus_tag="VGP50_06985"
     CDS             2165..3094
                     /locus_tag="VGP50_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV8027157.1"
                     /translation="MIRPRQVWPAGRLGSAPAGPARAPNIGRAIFYMALAMSLLPCLN
                     AAAKYLGHSYATVEIVWARYAGHFAYMVVVFLPRRGIALFATSRLSVQVLRSALLVSA
                     TGIYFTALHYTALPTAAIISFVSPFMMIALAGLILGEPIGPRRWAAAGIGFLGALVIL
                     RPGTGAVPLASFLLLISAGCNALYQILTRSLAARDSAETSNTYIAVVGFLLSSAAVPF
                     FWQAPRSLVDLLLFVSLGLFGGFGHYFIVKALEWGPAAVIAPLNYGQLIGTVIIGYFA
                     FGEFPDLWTWAGALIIVMSGLYIFYWERRRASA"
     gene            3186..4028
                     /locus_tag="VGP50_06990"
     CDS             3186..4028
                     /locus_tag="VGP50_06990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8027158.1"
                     /translation="MQGKVCVITGATSGIGLVAAERLAAMGARLLLVGRDPARGEAAL
                     RAIKARTPRADLRIVYGDLSRLSEMNRVAGEIAALQPRIDVLINNAGAMFQERGVTED
                     GLERSFALNHMSYFVLTNRLKDRLVAAGPARIVNTASGAHQGQSLDFADLQSERNYRG
                     LRVYGRSKLANILFTRELARRLTGTGLTVNCLHPGFVATRIGENNNLIFRVGLAIAKR
                     FALSPEKGAETIVYLASSPEVASVSGGYFYQSRPATPSTAARDDATAKRLWDESARIA
                     KLPA"
     gene            complement(4314..>5821)
                     /locus_tag="VGP50_06995"
     CDS             complement(4314..>5821)
                     /locus_tag="VGP50_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV8027159.1"
                     /translation="LISGPQVLGIMAARLVGRPVKLVLRREQMYGPVGHRAPTRQTLR
                     IAADAGGKLTAIAHHAKTTSSSFDDFFEPAADISHTLYASSAIATSHEVVRVDTGTPM
                     FMRAPGEATGSIALESAIDEAAEACGMDPLAFRLKNYAEVEPISGKPFSSKALRACYA
                     RGAERFGWSRRPLAPRQMRDDAGFLVGYGMGTATFPALMFAAEARAVVRSDGSGLMET
                     GAHDMGQGASTALAQIAADGLGLELEQVEFRTGTSDLPDAGIAGGSAHTATAGMAIHN
                     AGAAVIAKLADLATGNERSPLFGAGNAGVVARGGRLFRRDDEARSESYADILGRAGLK
                     EIEARGKSAADTAAHANYAMHAHGAVFAEVKVDPDLGQIRVTRMVGAFAAGRVINPRL
                     ARSQLLGGMIWGVSFALHEHAVMDRRSGRTLNADLAEYHVPVNADLPSLEALTIDEHD
                     PHVNALGIKGVGEIGITGSAGAVANAVWHATGIRVRRFPIRIDDLVMPASG"
BASE COUNT          922 a         1953 c         1929 g         1017 t
ORIGIN      
        1 cgcccgccct gcatggcatc gaccgcggcg cccgagagat agggaatggc gagcttcacc
       61 agctgcgcca ggatcagcat cgtcatgaag acgacgagcc gccgccggtc gccagcggca
      121 taatgccaca aggtgcggta aaggcccggc ggcttgccgg cggcgcgaga ggctgctgtt
      181 gccatggccg aagctaagcc caggcgcggt tagagaacgg ctaacaacgc caggcgcgcg
      241 ggaattattt cgcaggtcgc acgacacgct ttcgcgcttg catgacgatc ccgcgcgacg
      301 cacaagtcct gctccgccat cgcgggcggt aagaggagcc gaccttgatg ctcgatcccg
      361 agaagatcgc cgcgcttatt cgcgagaccg ctcaggcgga gatcctgccg cgctttcgca
      421 tgctgggcga gagcgacatc cgcgagaaga agccgggcga cttcgtgacc gcggcggatc
      481 tcgccgccga gcggcgcatg accgagtggc tgacggaagc ggcgcccgga tcgctggtgc
      541 tgggcgagga ggcggccgcc gaggatccgg ggcgcctcgc tctgctggcg agcgaggcgc
      601 gcgtctggat catcgatccc gtcgacggca ccgcaaactt cgcccgcggg gaaccgggct
      661 tcgcggtgat cctcgccgaa gtgcagaagg gcgaggtctg cgccggctgg atttacgatc
      721 ccctgagcga cgtcatggtg accgcagagg ccggcggcgg tgcctggtcg gcgggccggc
      781 ggcttacggt cgcgcgcgac gtcgctcccg cagagctcgt gggcggcgcc tatggccgca
      841 ccaaagcggg agtgcccgcc gccaaggcgc tcgaggccag cggccggatc ggccgcattc
      901 gcaatcgcgg ctgcggcggg ctcgaatact tggatctggc cctcggccgg gcgcatttct
      961 cgctccattc gcgctcgctg ccctgggacc atgccgcggg catgctgatc accgccgagg
     1021 cgggcggcgt cggcgcgttt ctcgacggca gccgctacga cgcgcgcatc atcgatcgcc
     1081 ctgtgctcgc cgcggcgagt gcggaggcat ggcagatcgt ccaggaggtc gtgagcgcgc
     1141 tcgcctgagc gcccggtcgt gcgcgcgcat gcgcggcagg cgtggctttt gtgcatccct
     1201 cgcggctttt cgtcacttcg cgtttatcga gaaaccgatc gttaactaca cgcgcgctat
     1261 gcgtccgtcg acgcggcgtg atgatgatgg agttgacgat gcgcagaggt tggatggcat
     1321 ggctggcggc gctcggatgg ttgctgccgg catcggcgat ggcgcagacc gtgcccggct
     1381 ggtcgatcgg acccgaagtc tactggtacg aatatcgcga gacaccgagc ttcgtctatc
     1441 aatggggtcc cttcggtggg gtcgatacca gctacacctt caagttcaat aacgtcttcg
     1501 ccacgctcga cggcgacgtc gatgtcggct acctcgacta caaggcgtcg ggctccggcc
     1561 ggctcaacgg catctggaac tacaagggtg acgtccgtgc gctgttcggc gtcgatcttg
     1621 gcgtctcccc gtctttctac atctcgccgt tcgccggtgc gggttatcgc gttctgttcg
     1681 agtcgcaagg cggccgcgcc accacgatcg ggcaagtcgc ctatgatcgc ctgtcgcagt
     1741 attactatct gccgctcggc atcgccacca gcatcacagc ggggagctgg gtccttcgtc
     1801 cgaccgcgga gtatgatttg tttatcggtg gcaatcagac tagctatctg tctcaggtcg
     1861 gtttcaatga ggacctgaac aatcgtcaga cggtcggcta tggtgcgcgt gccagcttgc
     1921 tggccgatac ccggacgccc tggggtcgta tcgccatcgg tccgttcttc cgctactgga
     1981 acatcgggtc gtctcgccag gtgccgggat tcatcggcaa caacgcggct gttttcttcg
     2041 agccacccaa caatacgatc gaggcgggcc tggcgctgca tttcctgttc taggcaaact
     2101 gttctaggca aaggacgccg aaatcgggct acaaatgccc tctcccgcga ggagagggga
     2161 atttgtgatc cgaccgagac aagtctggcc ggccgggcgc ctcggtagcg cgcccgccgg
     2221 gccggcgcgg gcgcccaaca tcggccgcgc catcttctac atggcgctcg ccatgagcct
     2281 tcttccctgt ctcaatgccg cggccaagta tctcggccac agctacgcga cggtggagat
     2341 cgtctgggcc cgctacgccg ggcatttcgc gtatatggtg gtggtgttcc tgccgcgccg
     2401 gggcatcgcg cttttcgcga cgagccggtt gtccgtgcag gtgctgcgct cggcgctgct
     2461 ggtgagcgcc accggcatct atttcacggc gcttcattac accgcgctgc cgacggcggc
     2521 gatcatcagt ttcgtctcgc ccttcatgat gatcgcgctc gccggtctca tcctcggcga
     2581 gcccatcggt ccgcgtcgct gggcggccgc cggcatcggc tttctcggcg ccctcgtcat
     2641 cctgcgcccc ggcacgggag cggtgccgct ggcctcgttc ctgctcctca tcagcgccgg
     2701 ctgcaacgcg ctctaccaga tcctgacgcg gagcctcgcc gcgcgggatt cggccgagac
     2761 ctccaacacc tacatcgccg tcgtcggctt tctcctgagc agcgccgccg tgccgttctt
     2821 ctggcaggcg ccgcgctcgc tcgtcgatct gctgctcttc gtgagcctcg gtctcttcgg
     2881 cggcttcggg cattacttca tcgtcaaggc gctcgaatgg ggcccggccg cggtgatcgc
     2941 gccgctcaat tacgggcagc tcatcggcac cgtgatcatc ggctatttcg ccttcggcga
     3001 attccccgac ctctggacct gggccggcgc tctcatcatc gtgatgagcg gcttgtatat
     3061 tttctactgg gaacgccgcc gcgcgagcgc atagcgctcg tcgcgggcgg cggcagcggt
     3121 tgcgcagcaa ccgcgagagc cgcacagtgg ggagtacgcg ggaagcagcg aaagggagag
     3181 tgctcatgca aggaaaggtc tgcgtcatca ccggcgccac gtcgggcatc gggctcgtcg
     3241 cggcagagcg gctggcggcg atgggcgcgc ggctcctcct ggtcgggcgc gatcccgcgc
     3301 gcggcgaggc ggcgctcagg gcgatcaagg cgcggacgcc gcgggcggat ttgcgcatcg
     3361 tctatggcga tctctcgcgc ctctccgaga tgaaccgcgt ggccggcgag atcgccgcgc
     3421 tccagccgcg gatcgacgtg ctcatcaaca acgccggcgc catgttccag gagcgcggcg
     3481 tgaccgagga cggcctcgag cgcagcttcg cgctcaacca catgtcctat ttcgtgctga
     3541 ccaaccgctt gaaggatcgc ctcgtcgcgg cggggccggc gcgcatcgtc aacacggcct
     3601 cgggcgcgca tcaggggcag agtctcgatt tcgccgacct gcaatccgag cggaattatc
     3661 gtggcttgcg cgtctatggc cgctccaagc tcgccaacat cctcttcacc cgcgagctgg
     3721 cgcggcgcct caccggaacg ggactcaccg tgaactgcct ccatcccggc ttcgtcgcca
     3781 cgcgcatcgg cgagaacaac aacctgatct tccgcgtcgg cctcgccatc gccaaacgct
     3841 tcgcgctgtc cccggagaaa ggggccgaga ccatcgtcta tctcgcatcc tcaccggagg
     3901 tggcgagcgt cagcggcggc tacttctatc aatcgcgccc ggcgacgccg agcacggccg
     3961 cgcgtgacga cgccacggcg aagcggctct gggacgaaag cgcgcgcatc gcgaagctgc
     4021 cggcttgaaa agaagattca ccaatgaggc ggtgagaaga aagaccgacc acagaggcac
     4081 agaggcacag agaagaaaaa tggtcgcttt gcgtgccgat tcataatctt ctcagcgtct
     4141 ctgcgcctca gtggtcattc atgccgcatg caccgcgctc gctctctcac tgcctcactg
     4201 cctccgtggt atatctttcg gaccgaacct aagccttctg cgccatcagc gcgttggacg
     4261 gcagcgtctt gtcgagcaca tggcgacggc ccagtagccg cgacacgcga aactcaaccc
     4321 gacgccggca tcacgagatc atcgatccgg atcgggaagc ggcgtacccg gattcccgtc
     4381 gcgtgccaca ccgcgttggc aacggccccg gccgagcccg tgatcccgat ttcgccgacc
     4441 cctttgatgc cgagcgcgtt cacatgggga tcgtgctcgt cgatcgtcag ggcttccaag
     4501 gacggcaggt cggcattcac ggggacgtgg tactccgcga gatcggcgtt gagcgtccgg
     4561 ccggagcgcc ggtccatgac ggcgtgctcg tggagagcga acgacacgcc ccagatcatg
     4621 ccgccgagga gctgactgcg cgcaaggcgc gggttgatga cccgccccgc cgcgaaggcg
     4681 ccgaccattc gggtaacgcg gatctgaccg agatcgggat cgaccttgac ctcggcgaac
     4741 accgcgccat gcgcatgcat ggcgtagttg gcgtgcgccg cggtgtccgc ggcgctcttg
     4801 ccgcgggctt cgatctcttt gaggccggcg cggccgagaa tatcagcata gctctcgctc
     4861 cgcgcttcat cgtcgcgacg gaagagacgg ccgccgcgcg cgacgacccc ggcattgccc
     4921 gcgccgaaca agggcgagcg ctcattgccg gtggcgagat ccgcaagctt ggcgatgacc
     4981 gccgcgcccg cattgtggat cgccatgccg gcggtggcgg tgtgggcaga gccgccggcg
     5041 atgcccgcat ccggcaggtc ggaggtgcct gtcctgaatt cgacctgctc gagctcgagc
     5101 cccagcccgt cggcggcaat ctgggcgagc gcggtcgacg cgccctggcc catgtcgtgg
     5161 gccccggtct ccatcaggcc ggacccgtcg ctgcgcacga ccgcacgcgc ctcggccgcg
     5221 aacatcagcg ccggaaatgt tgcggtgccc atgccatagc cgacaaggaa gccggcgtcg
     5281 tcacgcatct gtcgcggcgc gagcgggcgt cgcgaccagc cgaaacgctc cgctccccgc
     5341 gcgtagcacg cgcgcaaagc cttcgacgag aacggcttgc cggatatcgg ctcgacctcg
     5401 gcatagttct tcagccggaa ggccagcgga tccatcccgc aggcctcggc ggcttcgtcg
     5461 atcgcgcttt cgagcgcgat cgatcctgtc gcctctccag gggcgcgcat gaacatcggc
     5521 gtgccggtgt cgacccggac cacttcatgc gaggtggcga tggcggaact cgcataaagc
     5581 gtatgcgaga tgtcggcggc cggctcgaag aaatcgtcga agctgctcga tgtcgttttc
     5641 gcgtgatgag cgatggcggt cagcttgccg ccagcgtcgg cggcgatgcg gagcgtctgg
     5701 cgcgtcggcg cgcgatggcc gacaggcccg tacatttgct cacggcgcag cacgagcttg
     5761 accggccttc ccaccagccg ggccgccatg atgccgagga cctgcggccc ggagatcagg
     5821 c
//
LOCUS       DASYNS010000224         7217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_518091, whole genome shotgun sequence.
ACCESSION   DASYNS010000224 DASYNS010000000
VERSION     DASYNS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518091"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..818
                     /gene="thiE"
                     /locus_tag="VGP50_07000"
     CDS             153..818
                     /gene="thiE"
                     /locus_tag="VGP50_07000"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV8027160.1"
                     /translation="MRAPPDLRLYAIVDPERAGGRDLADLARSVAANGATLVQLRDKR
                     SETREMIETARAVKRALAPLAVPFLVNDRVDVALAAGADGVHLGPDDMPVADARALLG
                     PDAIVGLSVKTVDAAETARIDLIDYAGVGGVYATLSKEQKSAPIGTDGFARVAAALRR
                     RAPNLPVCGIAGIDAANAGAVIGAGADGIAVISALSLAPDPAAAARGLREVVDAMLAK
                     RGS"
     gene            complement(819..2270)
                     /locus_tag="VGP50_07005"
     CDS             complement(819..2270)
                     /locus_tag="VGP50_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEV8027161.1"
                     /translation="MNVMAQVTRAWPQHARFDGPIVMIGFGSIGKGTLPLLERHIAFD
                     RKKFVVIAPEDEDRRLLDDRQLRFIHTAVTKENYRDVLTPLLTAGPGRGMIVNLSVDT
                     SSVALIEFAKDLDCFYIDTVVEPWPGLYTDRNLSISARSNYALREGVLELRRRRPGGI
                     TAVSCCGANPGMVSWFVKQALLDVAADVGLKAEEPKTREDWARLMQRAGVTGIHIAER
                     DTQRARDPKPRGVFVNTWSVEGFCAEGMQPSELGWGTHEKTMPANARRHEFGCDAAIY
                     LTQPGAATRVRSWTPTAQSQHAFMITHNESISIADYFTLRENGKVAYRPTCHYAYHPC
                     DDAVLSLHEMAGNAWQAQPEWTILDEGEIVDGIDELGVLLYGHAKNAYWYGSQLSIEE
                     TRELAPYQNATGLQVTSAVLAGIVWMLENPDRGIVEADDMDFRRCLEIQRPYLGPVIG
                     RYTNWTPVDRRGELFPEELDRDDPWQFSNVLVK"
     gene            complement(2304..2867)
                     /locus_tag="VGP50_07010"
     CDS             complement(2304..2867)
                     /locus_tag="VGP50_07010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEV8027162.1"
                     /translation="MVKIRNEFARDIDARETLLDRVWGPARWQKTAERLREGRKPAEG
                     LSFIAEENGCLIGTVRLWRVGAGPARPALLLGPLAVEEARRGLGTGTALMQRAIAAAR
                     RLGHQAVLLVGDAPYYGRFGFSAEKTGALWLPGPFARHRLLGCELVPGALEGARGLIA
                     ATGRPAPSLDLLVARRGRTGPAARHAA"
     gene            complement(2888..4024)
                     /locus_tag="VGP50_07015"
     CDS             complement(2888..4024)
                     /locus_tag="VGP50_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEV8027163.1"
                     /translation="MTARIRDFLRNRQDDGPCLVVDLDIVRENYLGFAKALPDTRVYY
                     AVKANPAPEVLALLAGLGSSFDTASIPEIEMVLAAGAAADRISYGNTIKKERDIARAY
                     ALGVRLYAVDCQAEVDKVARAAPGSRVFCRILSDCVGAEWPLSRKFGCEPAMAVEVLE
                     AAHRLGLEPYGISFHVGSQQRNPHAWDRALASAAAVFRECGERGLTLSMVNLGGGFPT
                     RYLKNVPTVKTYGAAIFRALRRHFGNRIPETIVEPGRGMVGNAGVIETEVVLVSKKSE
                     SDDVRWVYLDIGKFGGLAETMDESIRYPIRTPRDTRDGEVGPCVLAGPTCDSADVMYE
                     RQPYLLPVTLEIGDKVLIEGTGAYTATYSAVAFNGFAPLKTFHI"
     gene            complement(4703..5662)
                     /locus_tag="VGP50_07020"
     CDS             complement(4703..5662)
                     /locus_tag="VGP50_07020"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEV8027164.1"
                     /translation="MFWPFLYGDVFSFAFWPYGYYDPFWALGPDFLLASIFAPGPYYG
                     YDYGNGAGLYGYAGSPDIYFGRRAARTSRGERARAEAAAQSCSGLAPGVTDLPIEQIR
                     RKIHPTGAAAAALDALSAAAFKGSDVVQASCPSEVPLTPVARLDAAEKRLEAMLQAVD
                     TMRDPLAKFYGALSDDERQRFDAMGKAGVSRAGSSAGGGDLASLCGQQDGATNLPVQR
                     IEQVVAPTAQQQGAFDDLKQAAKSAADELQASCPKEVPQAPVTRLDTVAKRLTAMIDA
                     MRTVRPKLESFYASLSDEQKAKFNTMGPPRDASSRSGQESGGQ"
     gene            5792..6403
                     /locus_tag="VGP50_07025"
     CDS             5792..6403
                     /locus_tag="VGP50_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027165.1"
                     /translation="MQAVKMRGGELVVGELTPSAKAAGVSAAESVVSCKPMRCEVMRC
                     EAVRGEPMSSTEPSMAAKAVTSKGATARAMPAEMAKAVSAKAMRRQAVEAAAAMKPTT
                     VKATTVKATAASSAAAAANSRRVGRNQQRAQRDARCQNSDCPLLHNSPPVQRHCVAMH
                     AATAIVGLFARANARRNRAVGSTSVLQTFEAGRGCSLGVKFRQ"
     gene            6400..>7217
                     /gene="thiD"
                     /locus_tag="VGP50_07030"
     CDS             6400..>7217
                     /gene="thiD"
                     /locus_tag="VGP50_07030"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV8027166.1"
                     /translation="MNASQRSPQKTATPIALTIAGSDSSGGAGIEADLKTFAALGVYG
                     ACVVTALTAQNTKGVFAVHEVPGDFIAAQINAVFSDLDVGAVKLGMLAHAAAIDVVAA
                     ALDRYRPPNVVLDPVMIASSGERLLHKGAIGRLRELVRRARVLTPNLAEAAALLEAPP
                     ARDEGQMRAQAEKLLALGAGAVLIKGGHGSGPESVDLLVTADGALRFAAPRAATRNTH
                     GTGCTLASAVAAALAKGLTLGEAVKLAKDYVTAAIEAADRLAVGSGRGPLHHFHA"
BASE COUNT         1143 a         2448 c         2453 g         1173 t
ORIGIN      
        1 acttgccgag ctgacacttg atctgcacga agaagggcac catgggcgtc tccgagcgtc
       61 cggaaatgag atcctacact acggatcgcg gtgcgggctg caagggccgg acgggacgta
      121 gtatctttgc gcgagccgga aggccaatcc caatgcgtgc accgcccgac cttcgcctct
      181 acgccattgt cgatccggag cgcgccggcg ggcgcgatct cgccgatctc gcgcgcagcg
      241 tcgccgcgaa cggagcgacg ctggtgcagt tgcgcgataa acgcagcgag acgcgggaga
      301 tgatcgagac ggcgcgggcg gtcaagcgcg cgctggcgcc gcttgccgtg ccgttcctgg
      361 tcaatgatcg cgtcgacgtg gcgcttgccg ccggcgccga cggcgtgcat ctcggccccg
      421 acgatatgcc tgtcgcggat gccagggcct tgctcgggcc ggacgcgatc gtcggccttt
      481 cggtcaagac cgtcgatgcc gccgagacgg cgcgcatcga cctcatcgac tatgccggcg
      541 tcggcggggt ctatgcgacg ctctcgaaag agcagaagag cgcgccgatc ggcacggacg
      601 gcttcgcgcg cgtcgccgcc gcgctgcgcc gccgcgcgcc gaatttgccg gtctgcggca
      661 ttgccggcat cgacgccgcc aatgccggcg ctgtcatcgg cgccggcgcc gacggcatcg
      721 ccgtcatttc cgcgctgtcg ctggcgcccg atcccgccgc cgctgcgcgc ggcttgcgcg
      781 aggtcgtcga tgcgatgctg gcgaagcgcg gctcgtgatc acttcaccag gacgttgctg
      841 aactgccagg gatcgtcgcg atcgagctcc tccgggaaca gctcgccgcg gcgatcgacc
      901 ggcgtccagt tggtatagcg gccgatcacc ggcccgagat aaggccgctg gatctccagg
      961 cagcgccgga aatccatgtc gtcggcttcg acaatgccgc ggtcggggtt ctcgagcatc
     1021 cagacgatgc cggcaagcac tgccgaggtc acctgcagcc cggtggcatt ctgataaggc
     1081 gcaagctccc gcgtctcctc gatggagagc tgcgagccgt accaataggc gttcttggca
     1141 tggccataga gcagcacgcc gagctcatcg atgccgtcga cgatctcgcc ctcgtctagg
     1201 atcgtccact ccggctgcgc ttgccacgcg tttccggcca tttcatgcaa ggacaatacg
     1261 gcgtcgtcgc acgggtgata ggcgtagtgg caggtcggcc gataggcgac cttgccgttc
     1321 tcgcgcagcg tgaagtagtc ggcgatcgag atcgactcgt tgtgggtgat catgaaggcg
     1381 tgctgcgact gtgcggtcgg ggtccaggag cgcacgcgcg tcgcggcacc cggctgggtc
     1441 agatagatcg ccgcatcgca gccgaattcg tgccggcgtg cgtttgccgg catcgtcttc
     1501 tcatgcgtgc cccagccgag ctccgagggc tgcatgccct cggcgcagaa accttcgacc
     1561 gaccaggtat tgacgaacac gccgcgcggc ttgggatcgc gcgcgcgctg cgtatcgcgc
     1621 tcggcgatgt ggatgccggt gaccccggcg cgctgcatca gccgcgccca atcctcacgc
     1681 gtcttcggct cctcggcctt cagcccgacg tcggcggcga cgtcgagcag agcttgcttg
     1741 acgaaccagg aaaccatgcc cggattggcg ccacagcagc tcacggcggt gatgccgccg
     1801 ggccggcgtc ggcgcaattc cagcacgcct tcgcgcagcg cgtagttcga gcgcgccgag
     1861 atcgaaagat tgcggtcggt gtaaagcccg ggccatggct cgaccacggt gtcgatgtag
     1921 aagcagtcga gatccttggc gaactcgatc agcgcgaccg aactggtatc gaccgaaagg
     1981 ttgacgatca tgccgcggcc cggcccggcg gtaaggagcg gggtcagcac gtcgcggtag
     2041 ttctccttgg tgacggcggt gtggatgaaa cggagctggc ggtcgtcgag cagccgacga
     2101 tcctcgtcct ccggcgcgat caccacgaat ttcttgcgat cgaatgcgat gtgacgctcg
     2161 aggagcggca gcgttccttt gccgatcgag ccgaacccga tcatcacgat cgggccgtcg
     2221 aagcgtgcgt gctggggcca ggcgcgggtc acttgggcca taacattcat gctaatcgct
     2281 cctaatgcaa ttcgcctgtc gcgtcaggcg gcgtggcgcg cggccggacc ggtgcggccg
     2341 cggcgcgcaa ccaggaggtc gaggctcggc gctggacgcc ccgttgcggc gatgagcccg
     2401 cgcgcgccct caagcgcgcc cggcacgagt tcgcagccga gcaggcgatg ccgcgcgaac
     2461 gggccgggca gccacagggc gccggtcttc tccgccgaga agccgaagcg tccgtagtac
     2521 ggtgcgtcgc cgaccagcag cacggcctga tgcccgagcc gccgcgccgc cgcgatcgcc
     2581 cgctgcatca gggcggtccc ggttccgagg ccgcggcgcg cttcctcgac tgcgagcggc
     2641 ccgagcagca aagccggccg ggccggcccg gcgccgacgc gccagagccg gaccgtgccg
     2701 ataaggcagc cgttctcctc ggcgatgaag gaaagccctt cggcgggctt gcgcccttcg
     2761 cgcagccgct cggcggtctt ctgccaacgc gccggccccc agacacggtc gagcagcgtc
     2821 tcgcgcgcgt cgatgtcgcg ggcgaactcg ttgcggatct tcaccatggc ccactccctt
     2881 cgcgcgatca gatgtggaag gtcttgagcg gcgcgaagcc attgaaggcg acggccgagt
     2941 aggtcgcggt gtaggcgccg gtcccttcga tcagcacctt gtcgccgatc tcgagcgtca
     3001 ccggcagcag atagggctga cgctcataca tcacgtccgc cgaatcgcag gtcggcccgg
     3061 ccagtacgca gggcccgacc tcgccgtcgc gggtgtcgcg cggcgtacgg atcggataac
     3121 ggatcgactc gtccatggtc tcggcgaggc cgccgaattt gccgatgtcg aggtagaccc
     3181 agcgcacgtc gtcgctctcg ctcttcttgg agacgagcac gacttccgtc tcgatcacgc
     3241 cggcattgcc gaccatgccg cggccgggtt cgacgatggt ctccgggatg cgattgccga
     3301 aatggcgccg cagcgcgcgg aagatcgccg caccgtaggt cttcaccgtc ggcacgttct
     3361 tcaggtacct ggtcgggaag ccgccgccga gattgaccat cgacagcgtc aagccgcgct
     3421 ccccgcactc gcggaacacc gcagcggcgg aggcgagcgc gcggtcccag gcgtgcggat
     3481 tgcgctgctg cgatccgaca tggaacgaga tgccgtaggg ctcgaggccg agccggtgcg
     3541 ccgcctccag cacctcgacc gccatcgccg gctcgcagcc aaacttgcgc gacagcggcc
     3601 actccgcccc gacgcagtca gagaggatgc ggcagaacac ccggcttccc ggcgccgcgc
     3661 gcgcgacctt gtcgacctcc gcctggcagt cgaccgcgta gaggcgcacg ccaagcgcat
     3721 aggcgcgcgc gatgtcgcgc tccttcttga tggtgttgcc ataggagatc cggtccgcgg
     3781 cggcgccggc ggcgagcacc atctcgatct cggggatcga agcggtgtcg aacgaggagc
     3841 ccagcccagc gagcagcgcc agcacctcgg gcgcgggatt ggccttcacc gcgtagtaca
     3901 cgcgcgtgtc cggcagcgcc ttggcgaagc cgaggtaatt ctcgcggacg atatcgagat
     3961 cgacgacgag gcagggcccg tcgtcttggc gattgcgcag gaagtcgcgg atgcgcgcgg
     4021 tcatgggcgc tctcctcacc aggttcaatg agccttgcgg ctcggattga aattcgggcg
     4081 gaaagaaccg cccggcgtca gcgggtgagg gtcggcgcct cgccgtgcga tggagacacg
     4141 agcgaaggcg acgggctctg cacccgagat gctgccttgg tttggagcgg gaagacccgt
     4201 tccgcacggc cggcaaggat ggacaagcct ctgaggtgcc gacctttgga gggccggcga
     4261 gaccagagaa gcccgctccg tcgttgcttt aagccgcgtc ccccgttgag cgcggggtgc
     4321 gccggtttcg cctccggctg ccggttaagt ggttcggagc tcaggcggcc aaatctctcg
     4381 ccgtcgggtt tggagtccct tggcttcgag tctcgacctc actccgggcg gctgtccggc
     4441 ctcttgtccg gatgcccacc aaccggcacg cggccacagg cacgtgcgaa attgggcgag
     4501 cgcgacataa gcgcgatggc gcagcgatgc aagcattttt ttccgcggcg ccgatttttt
     4561 ccgttcggcg cgcggaagtg gttagcgtga cattgccgcg cggcgcgtgt ccgcgctcgt
     4621 ttcgccagcg cgcaggcttg agcgaggcgg ccacgatcag tgtccgcgtc gccaagctgc
     4681 gccatccgct cgaatggatt tcctattggc cgccgctctc ttggccgctc cgcgaggatg
     4741 catctcgcgg cgggcccatg gtgttgaact tcgccttctg ctcgtcgctg agcgatgcgt
     4801 agaagctctc gagcttcggg cgaacggtcc tcatcgcatc gatcatggcg gtgagccgct
     4861 ttgccaccgt gtcgagccgc gtcaccggag cttgcggcac ctctttgggg caggacgcct
     4921 gcagttcgtc ggccgcgctc ttcgccgcct gcttcaggtc gtcgaatgcg ccctgctgtt
     4981 gcgcggtcgg cgccaccacc tgctcgatgc gctgcaccgg cagattggtc gcgccgtcct
     5041 gctggccgca gagcgatgcg agatcgccgc cgccggcgct cgaaccggct cgcgacacgc
     5101 cggccttgcc catggcgtcg aagcgctgtc gctcatcgtc gctgagcgcg ccatagaact
     5161 tcgccagcgg gtcgcgcatg gtgtcgaccg cctgcaacat ggcttcgagc cgcttttcgg
     5221 cagcgtcgag tcgcgccacc ggagtcagcg gaacctcgct cgggcacgat gcctggacga
     5281 cgtcgctccc cttgaacgcc gccgcgctca aagcgtcgag ggcggccgcc gccgctccgg
     5341 tcggatgaat cttcctacgg atctgctcga tcggcagatc ggtcacgccc ggtgcaagcc
     5401 cgctgcagct ctgcgccgca gcctcggctc gcgcgcgctc accgcggctc gttcgtgctg
     5461 cgcggcggcc gaagtaaatg tcgggagacc cagcatagcc gtaaaggccg gcgccatttc
     5521 cgtagtcgta gccataatac gggcctggcg cgaagatgct ggcgaggagg aagtccgggc
     5581 ccaacgccca gaacggatca tagtagccgt aaggccagaa cgcgaagctg aagacgtcgc
     5641 cgtagaggaa cggccagaac accgggccgg cccagccgcc ccatccccaa ccccaattgt
     5701 tccatccggc tccccagaat tggccgcccc agcggttcca attgcgtccg ttgccgaatg
     5761 cattacggtt gaagccggcg cgattgaact gatgcaggcc gtgaaaatgc gcggcggcga
     5821 gttggttgtg ggcgaactga ccccgagcgc caaagcggcc ggcgttagcg ccgccgagtc
     5881 cgtcgtgagc tgcaagccca tgcgctgtga ggttatgcgt tgcgaggccg tgcgcggcga
     5941 gcccatgagc agcactgagc cctccatggc ggccaaagcc gtgaccagca aaggagcgac
     6001 tgccagggcc atgcccgccg agatggccaa ggccgtgtcc gccaaagcca tgcgccgcca
     6061 ggccgtggaa gccgccgccg ccatgaaacc caccaccgtg aaagccacca ccgtgaaagc
     6121 caccgccgcc tcgtccgccg ccgccgcggc caacagccga cgcgtcggac gcaaccagca
     6181 gcgcgcacag cgcgacgctc gttgccagaa ctccgattgt ccgcttttgc ataactctcc
     6241 tccggtgcag cggcattgcg ttgcgatgca tgccgcgacg gccatcgtcg gccttttcgc
     6301 ccgcgcgaac gcgcgtcgaa atcgagctgt gggctccacg tccgtactac aaacttttga
     6361 ggccggtcgc ggttgctcgc tgggggttaa atttcggcaa tgaatgcatc gcagcgaagc
     6421 ccgcagaaaa ccgcgacgcc gatcgccctg acgatcgccg gctcggattc gagcggcggt
     6481 gccggcatcg aggccgatct caagaccttt gccgcgctcg gggtctatgg cgcgtgcgtc
     6541 gtcaccgcgt tgacggcgca gaacaccaag ggggtcttcg ccgttcatga ggtgcccggc
     6601 gacttcatcg cagcgcagat caacgcggtg ttctccgacc tcgatgtcgg cgcggtcaag
     6661 ctcggcatgc tggctcatgc ggcggcaatc gacgtggtcg ccgccgcgct cgaccgctac
     6721 cgcccgccca atgtggtgct cgatccggtg atgatcgcca gctccggcga gcggttgctg
     6781 cataaaggcg cgatcggccg gctgcgggag ctggtccggc gcgcgcgcgt gctcacgccc
     6841 aaccttgcgg aggccgccgc gctcctcgaa gcgccgcctg cgcgcgacga ggggcagatg
     6901 cgcgcgcagg cggaaaagct gctcgcgctc ggcgccggcg cggtgctgat caaaggcggc
     6961 catggtagcg ggccggaaag cgtcgatctt ctggtgaccg cggacggggc tctgcggttc
     7021 gcagcgccgc gggcggcgac gcggaacacc cacggcaccg gctgcacgct cgcttcggcg
     7081 gtcgccgccg cgcttgccaa agggctgacg ctcggcgagg cggtgaaact ggccaaggac
     7141 tacgtgaccg cggcgatcga agcggcggac cggctcgctg tcggctccgg ccgtggtccg
     7201 ctgcatcatt tccacgc
//
LOCUS       DASYNS010000225         7739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3951041, whole genome shotgun sequence.
ACCESSION   DASYNS010000225 DASYNS010000000
VERSION     DASYNS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3951041"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(389..1318)
                     /locus_tag="VGP50_07035"
     CDS             complement(389..1318)
                     /locus_tag="VGP50_07035"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8027167.1"
                     /translation="MSALFCFGLGYTAGFLARALQGEGWRVGGTSRDAASVKRLAAEG
                     FVMHPLAPGRPLADAASALGGVTHILVSVPPDASGDPVLAAHAADLAALRGLQWLGYL
                     STTGVYGDRGGAWVDETDRLEPTGERGRRRVAAEAQWLALQREHGIPVHIFRLAGIYG
                     PGRSALDTVRQGRAQRIDKPGQVFSRIHVADIVQVLRASMARPRLRAIYNVCDDDPAA
                     PEAVIAYACELLGVTPPPLISFDAAKLSLMALSFYDDNKRVSNRRMKEELGVSLRYPS
                     YRDGLKALLNGPSGETPSPLVGEGGAQRGALRG"
     gene            1672..2817
                     /locus_tag="VGP50_07040"
     CDS             1672..2817
                     /locus_tag="VGP50_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV8027168.1"
                     /translation="MKPLVVAIAFMALLAGAGIVYQNFASSSARSEAAPGPYAAPVVV
                     ADATRAAMPIRLDAVGTAQTIASVAIKSRIDGQITEVKVHDGQYVKAGDVLFLLDSRA
                     SEAQVGQMEAQLARDQAQLVNARRDVDRFAPLVAKDFVSHQQYDTATTTARALEATVK
                     GDQATLENSRVVLTYYTITASMDGRIGTIGLKAGNNVKANDVPLLTLNQIKPVWVAFS
                     LAQSDFPALRAAMAKGDVEVDAAPPGDSAPPEKGRVAFFENTIDATTGTIAMKAVFDN
                     AQERLWPGEFVNVSIMLGMEQDVLVVPQAAVQVGQNNTYVFVVKADDTVELRPVRLSR
                     NLDGKSIIASGIEAGERVVTDGQLRLSNGTRVETRPAAEPTKPGSPS"
     gene            2814..5951
                     /locus_tag="VGP50_07045"
     CDS             2814..5951
                     /locus_tag="VGP50_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV8027169.1"
                     /translation="MTLPELCIRRPVMTTLLMLAFVIFGLFGYRLLPVAAIPRVDFPT
                     IVVAAQLPGASPESMASSVATPLERQFSTIAGLDSMISTSGQGITAITMQFNLDRNID
                     GAALDVQSAITTAARLLPIEMTIPPSFQKVNPADQPVMYICLNSDTLPLSAVDEYAET
                     LMAQRISMLLGVAQVQVFGSQKFAVRIQVKPDALAAKGLALADVRNAVAAANSNTPVG
                     QLSGTDQTFTLEANAQLANAAQFQKIPIAYKNGAPIRLGDVAVVIDSVENNKIASWFN
                     GKRGVILSVMRQPDANTVEVVDSVKRLLPVFRAQIPPSVSMNVMNDRSTSIRNAVDDV
                     QFTLMLTVGLVVLVIFLFLRNVTATVIPALALPVSIIGTFAGMYVLGYSIDNLSLMAL
                     TLSVGFVVDDAIVMLENIVRHIENGERPFEAALKGSREVAFTIVSMTLSLVAVFIPVL
                     FMSGVVGRVFREFAVTISLTILISGIVSLTLTPMLCSRFLKTHHGPQNRFYRASEAVF
                     DGMLDAYEWMLRRVLNWKLATLVVTILSVLLTGYLYGAISKGFFPSEDTGLLFAFTEA
                     AQDISYDSMVAHQQRVADIVRADPNVEDVTSVVGATSFSPALDNGRVLVQLKPRDQRP
                     RNADQVIQQLRRKVAQEPGMKVYFQSLQNIQLGGRLAKSTYQYTIQDGDTDELYRLAP
                     LLQDKIAEIPGFQDVNSDLQLRNRQAVVDVDRDKAAQFGLTIDLVKNALYDAFGTRQI
                     STIYAPTNDYQVIIEVDPNYQRDPTDLSHFFLRAGNGQTIPLSAVASLRPSIGPVLVN
                     HQGQLPSVTISFNLDSNRISLGDATAAISRIERQLNLPVTVSTGFQGTAQVFRDSLRG
                     QGLLLMAAVLVIYMVLGILYESFIHPITILSGLPSAGLGALLTLMLVHMDLSVIAIIG
                     IVMLIGIVKKNAIMMIDFALERQRAGDITAEEAIHEACLLRFRPIMMTTMAAIMGALP
                     IAVGVGAGSELRQPLGMAVVGGLLVSQLLTLFITPVIYVYLEQARHYWNRGALPAAAP
                     AAASLHGGHATLPGE"
     gene            5992..6753
                     /locus_tag="VGP50_07050"
     CDS             5992..6753
                     /locus_tag="VGP50_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027170.1"
                     /translation="MSPATGARAQAAGEDPATLHDGCVALAHSAPQEGLDRAELWRSQ
                     GGGFFADHCAAMALYGLGRYSDAARGFEVLATAMMAAPKLQRAQTLDQAGQAWLAADD
                     AARAKADFDAALALDKDDPDLLIDRAEAQAALKKYWEAIDDLNRVLELAPKRADAYVY
                     RASAYRAVDAIDLALEDVERALAIEPNSTLGLLERGNARRLKGNAAGAREDWLRIIAL
                     APESAAAAAARVNIERLSAKSETPPEDAAAKPKMP"
     gene            complement(7125..>7739)
                     /locus_tag="VGP50_07055"
     CDS             complement(7125..>7739)
                     /locus_tag="VGP50_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEV8027171.1"
                     /translation="VLQEKALDFTMKVEKTWERRPEFLALNPAGEVPVLIEPDGTVLA
                     EASAIAEYLDEVYRDKLLLGINPVDRAEVRRLVAWFDQKMNHEVTENLVGEKIMKRFL
                     GFGEPNSAAIRAGKANLGYHLDYIGYLGERRRWLAGDHFSLADITAAAHLSALDYLGD
                     VPWDAHEPAKEWYARIKSRPSFRPILADHIPGAPPPKHYGDLDF"
BASE COUNT         1304 a         2716 c         2457 g         1262 t
ORIGIN      
        1 ccggacgtgc cgtggcagac gagcctggcg ctcgccgatc gcctcgcgag cgacgacgtc
       61 cggataaact tggtgaagga cggtgatcat cggctctcgc gcgatgccga cctcgctctt
      121 ctcggacgga ccgtcgagag catgctcggc gggtgacacg tcatggatga attgacccgg
      181 gcgaagtggc tgcgctggaa cgaggtggtc gagatccacc ttcgctcccg cgacgatcgc
      241 gtggccgagt tcctcgccgg cggcgctgac ctgcacccca tcgaatcctc ggagatcact
      301 gatcccctct ccccttgtgg gagagggagg ggcccgccgc gaagcggcgg gagggtgagg
      361 ggatggaaca gcgagcgagc ccaacccctc accctcgcag cgctccgcgc tgggcccctc
      421 cctctccaac aaggggagag ggagtctcac cactcggccc attcagcaac gccttcagcc
      481 catcgcgata actcggatag cggaggctga cgccgagttc ctccttcatg cggcggttcg
      541 agacgcgctt gttgtcgtcg taaaagctca gcgccatgag cgagagcttc gccgcatcga
      601 acgagatgag cggcggcggc gtcacgccga gcaattcgca ggcataggcg atcaccgcct
      661 cgggcgctgc cgggtcgtcg tcgcagacat tgtagatcgc gcgcagacgg ggccgcgcca
      721 tcgacgcgcg cagcacctgg acgatgtcgg cgacgtgaat gcgcgagaac acctggcccg
      781 gcttgtcgat gcgctgcgcc cgcccctggc gcaccgtgtc gagcgcgctg cgcccgggcc
      841 catagattcc cgcgaggcgg aagatatgca ccggaatccc gtgctcgcgc tggagcgcca
      901 gccattgcgc ctccgccgcg acgcgccgcc gcccgcgctc gccggtgggt tcgagcctgt
      961 cggtctcatc gacccacgcg ccgccgcgat cgccatagac gccggtggtc gagagatagc
     1021 ccagccattg caggccgcgg agcgccgcga gatcggcggc atgcgccgcc agcacggggt
     1081 cgccgctcgc gtcgggcggc acggagacca ggatatgcgt gacaccgccg agcgcgctcg
     1141 cggcgtcggc gagcggccgt cccggcgcga gcggatgcat gacgaagccc tcggcagcga
     1201 ggcgcttcac gctcgccgcg tcgcggctgg tgccgccgac gcgccagccc tccccctgca
     1261 acgcgcgggc gagaaaaccc gccgtgtagc cgagaccgaa gcagaaaagc gcgctcattc
     1321 ggcagcagcc tggcgcgctt cgccaccgag cgcccgctcc atcgtgtaca cggggcaatt
     1381 gcggacaggg agcgccgcgt tctcgaagcc cgtcatgccc gcgtccgctc cctggggcgc
     1441 gtcctctgtt tcttgaccgt ttccgcgcat tcctaaaggg aatgttaaca gagcgcgcgc
     1501 cataccctag ctccttcgcg cgaggatagg gagggcagac catggtacat gcaccgaggt
     1561 cgaacaggcg ctgtccgagc ctgcgcttat cgttggcggc ggcttcgctt atactggcgc
     1621 gatcacggat aacggctcgc tgaccgagcc gtcgcgggga aggcgggggt cgtgaaacct
     1681 ctcgttgtgg cgatcgcgtt catggcgctt ctggcgggcg ccggcatcgt ctatcagaac
     1741 ttcgcgtcga gctcggcacg gtccgaggcc gcgccgggcc cctatgccgc gcccgtggtg
     1801 gtggccgacg cgacccgcgc ggcgatgccg atccgcctcg atgccgtcgg cacggcgcag
     1861 accatcgcca gcgtcgccat caagtcgcgc atcgacggcc agatcacgga agtgaaggtc
     1921 catgacggcc aatatgtgaa agccggcgat gtgctgttcc tgctcgacag ccgcgcgtcc
     1981 gaggcgcagg tcgggcagat ggaagcgcag ctggcgcgcg atcaggcgca gctcgtcaac
     2041 gcgcgccgcg acgtcgaccg cttcgcgccg ctcgtcgcca aggacttcgt gtcgcatcag
     2101 caatacgaca cggcgacgac caccgcccgc gcgctcgagg ccaccgtcaa gggcgatcag
     2161 gccacgctcg agaattcccg ggtggtgctg acctattata cgatcacggc gtccatggac
     2221 gggcggatcg gcacgatcgg cctcaaggcc ggcaacaacg tcaaggccaa tgacgtgccg
     2281 ctgctgaccc tcaaccagat caagccggtc tgggtggcgt tctcgctggc gcaaagcgat
     2341 tttcccgcgc tccgcgcggc catggcgaaa ggcgacgtcg aggtcgacgc ggcaccgccg
     2401 ggcgattctg cgccgccgga gaaggggcgc gtcgcgttct tcgagaacac catcgacgcg
     2461 acgaccggca cgatcgccat gaaggcggtc ttcgacaatg cgcaggaaag gctctggccc
     2521 ggcgagttcg tcaacgtctc gatcatgctc ggcatggagc aggacgtgct ggtcgtaccc
     2581 caggccgcgg tccaggtcgg gcagaacaac acctatgtct tcgtcgtcaa agccgacgac
     2641 acggtcgagc tgcgcccggt ccgcttgagc cgcaacctcg acggcaaaag catcatcgcc
     2701 agcggcatcg aagcaggcga gcgcgtggtg acggatggac agctcaggct gagcaacggc
     2761 acgcgcgtcg agactcgtcc ggcggcggag ccgaccaagc ccggtagtcc ctcatgacgc
     2821 tgccggagct atgcatccgc cgtccggtga tgacgacgct cttgatgctg gccttcgtca
     2881 tcttcggtct cttcggctat cgcctgctgc cggtggcggc catcccgcgc gtcgacttcc
     2941 cgacgatcgt cgtcgcggcg cagctcccgg gagcaagtcc cgaatccatg gcctcgtcgg
     3001 tggcgacgcc gctcgagcgg cagttctcga ccatcgccgg cctcgattcc atgatctcga
     3061 ccagcggcca gggcatcacc gcgatcacga tgcaattcaa tctcgaccgc aacatcgacg
     3121 gcgccgcgct cgacgtgcag tcggcaatca ccaccgccgc ccggctgctg ccgatcgaga
     3181 tgacgatccc gcccagcttc cagaaggtca atccggcgga ccagccggtg atgtacatct
     3241 gcctcaactc cgacaccttg ccgctctcgg ccgtcgacga atatgccgag acgctgatgg
     3301 cgcagcgcat ctcgatgctc ctgggcgtgg cccaggtcca ggtcttcggc tcgcagaaat
     3361 tcgccgtccg catccaggtg aagccggacg ccctcgccgc caagggcctg gcgctcgccg
     3421 atgtgcgcaa cgccgtcgcc gccgccaact ccaacacgcc ggtcggccag ctcagcggca
     3481 ccgaccagac cttcacgctc gaggccaatg cgcagctcgc caacgccgcc cagttccaga
     3541 agattccaat cgcctacaag aacggcgcgc cgatccgcct cggcgacgtc gccgtcgtca
     3601 tcgacagcgt cgagaacaac aagatcgcga gctggttcaa cggcaagcgc ggggtgatcc
     3661 tgtcggtgat gcgccagccc gacgccaaca ccgtcgaggt cgtggacagc gtgaagcgcc
     3721 tcctcccggt gttccgcgcg cagatcccgc cctcggtcag catgaacgtg atgaacgacc
     3781 gctcgacctc gatccgcaac gcggtcgacg acgtgcagtt caccttgatg ctcacggtcg
     3841 gcctcgtcgt gctggtgatc ttccttttcc tgcgcaacgt cacggcgacc gtcatcccgg
     3901 cgcttgcgct gcccgtgtcg atcatcggca ccttcgccgg gatgtatgtc ctgggctact
     3961 cgatcgacaa cctgtcgctg atggcgctca ccctctcggt cggcttcgtc gtcgacgatg
     4021 ccatcgtcat gctcgagaac atcgtgcgcc atatcgagaa tggcgagcgt cccttcgagg
     4081 cagcgctcaa gggctcgcgg gaagtcgcct tcaccatcgt gtcgatgacg ctgtcgctcg
     4141 tcgcggtgtt cattcccgtg ctcttcatga gcggtgtcgt cggccgcgtc ttccgcgaat
     4201 tcgccgtcac catcagcctg acgatcctca tctccggcat cgtctcgttg acgttgacgc
     4261 cgatgctgtg ctcacgcttc ctcaagaccc atcacgggcc gcagaaccgg ttctaccgcg
     4321 catccgaagc ggtattcgac ggcatgctcg acgcctatga atggatgctg cgccgggtct
     4381 tgaactggaa gctcgcgacg ctggtcgtga ccatcctctc ggtcttgctc acgggctatc
     4441 tgtacggcgc catctccaaa ggcttcttcc cgagcgagga cacgggcctc ctcttcgcct
     4501 tcaccgaggc ggcgcaggac atttcctacg actccatggt cgcgcatcag cagcgggtcg
     4561 ccgacatcgt gcgcgccgat cccaacgtcg aggacgtgac ctcggtggtg ggcgccacca
     4621 gcttcagccc ggcgctcgac aacggaaggg tgctggtcca gctcaagccg cgcgatcagc
     4681 gcccgcgcaa cgccgaccag gtgatccagc agctgcgccg caaggtggcg caggagccgg
     4741 ggatgaaggt ctatttccag agcctgcaga acattcagct cggcggccgg ctcgccaaga
     4801 gcacctatca atacacgatc caggacggcg acaccgacga gctctaccgc ctggcgccgc
     4861 tgctgcagga caagatcgcg gagatccccg gcttccagga cgtgaactcg gatctccagc
     4921 tgcgcaaccg ccaggccgtg gtcgatgtcg accgcgacaa ggcggcgcag ttcggcctca
     4981 ccatcgacct ggtgaagaac gcgctctacg acgccttcgg cacgcggcag atctcgacca
     5041 tctatgcgcc caccaacgac taccaggtga tcatcgaggt cgacccgaat taccagcgcg
     5101 acccgacgga cctttcgcat ttcttcctgc gcgccgggaa tggccagacc attcccctct
     5161 ccgccgtcgc gtcgcttcgt ccgagcatcg ggccggtcct cgtgaaccac cagggacagc
     5221 tcccctcggt gaccatctcc ttcaatctcg actccaaccg catatcgctg ggcgatgcca
     5281 ccgccgcgat cagccgtatc gagcgccaac tcaacctgcc ggtcacggtg tcgaccggct
     5341 tccaaggcac ggcgcaggtc ttccgcgatt ccctgcgcgg ccagggactg ctgctgatgg
     5401 ccgcggttct cgtgatctac atggtgctcg gcattctcta tgagagcttc atccatccga
     5461 tcacgattct ctcgggcctt ccctccgccg gcttgggcgc gcttttgacg ctcatgctcg
     5521 tgcacatgga tctctcggtc atcgccatca tcggcatcgt catgctgatc ggcatcgtca
     5581 agaagaacgc catcatgatg atcgacttcg ccctcgagcg gcagcgtgcc ggcgacatca
     5641 ccgccgagga ggcgatccac gaggcctgcc tcttgcgctt ccgtcccatt atgatgacca
     5701 ccatggcggc gatcatgggc gctctcccca tcgccgtggg cgtcggcgcc ggctccgagc
     5761 tgcgccaacc gctcggcatg gcggtggtcg gcggactcct cgtctcgcag ctcctcacgc
     5821 tcttcatcac gccggtgatc tacgtctatc tcgagcaagc gcggcactac tggaatcgcg
     5881 gggcgctacc cgccgccgcg ccggcagcgg cttcgctcca cgggggccat gccacccttc
     5941 ccggcgaata ggcgacggct tctcgtcccg gcggcggtcg cgcttctcgc catgagcccg
     6001 gcgacggggg cgcgcgcgca ggccgccggc gaggacccgg ccacgctgca cgacggatgc
     6061 gtggcgctgg cgcacagcgc tccccaggaa ggcctcgatc gcgccgagct ctggcgcagc
     6121 caaggcggcg gcttcttcgc cgaccattgc gcggccatgg cgctctacgg gctgggccgc
     6181 tacagcgatg cggcgcgcgg cttcgaggtg ctggcaacgg cgatgatggc ggcgcccaag
     6241 ctgcagcgcg ctcagaccct cgaccaggcc ggccaggcct ggctcgccgc ggacgacgcg
     6301 gcgcgcgcca aggcggattt cgacgcggcg ctggcgctcg acaaggacga tcccgatctg
     6361 ctcatcgacc gcgccgaggc gcaggcggcc ctcaagaaat attgggaagc gatcgacgat
     6421 ctgaaccgag tcctcgagct cgcgcccaag cgcgccgacg cctatgtcta tcgcgcctcg
     6481 gcctatcgcg ccgtcgacgc gatcgatctc gcactcgaag acgtcgagcg cgcactcgcg
     6541 atcgagccca actccaccct cggcctcttg gagcgcggca acgcgcggcg gctcaagggc
     6601 aatgccgccg gcgcccgcga ggattggctg cgcatcatcg cgctcgctcc cgagagcgcc
     6661 gccgccgccg ccgcccgggt caacatcgag cgcttgagcg ccaagagcga aaccccgccc
     6721 gaggatgcgg ccgccaaacc caagatgcct taggtcgtgt gaacattcgc gtctcatggc
     6781 ggttccgccc cgtctctggc ggtcgcacca agacaaacaa gaagatgaac gcggaggtcg
     6841 cggaggcgcc gcggaggtcg cggaggaaca accatcctcg gcgacctccg cggaaccacc
     6901 gcgtcctctg cgttccgcga tcgccaacaa agggctcgct ccccgaccga gtcacgtgac
     6961 gaatgcgacg cggcccaggg cagatttttg cccaagtaga tccgctttta gttcgtcatc
     7021 cccgcgaaag cggggaccca ggggccaccg gcacgtcgct caccctgggt ccccgcttcg
     7081 cggggacgac gaagcagaat gcttcgacat gaagaggagg tgctctaaaa atccagatcg
     7141 ccgtaatgct tcggcggcgg cgcgcccgga atgtggtcgg cgaggatggg gcggaagctc
     7201 gggcgcgact tgatccgcgc ataccattcc ttggccggct catgcgcgtc ccaaggcacg
     7261 tcgcccagat agtcgagcgc cgagagatgg gccgccgcgg tgatgtccgc gagcgagaaa
     7321 tgatcgcccg cgagccagcg ccgccgctcg ccgagatagc cgatgtaatc gaggtggtag
     7381 ccgagattgg ccttgccggc gcggatcgcg gcggagttgg gctcgccgaa gccgaggaat
     7441 cgcttcatga tcttctcgcc gacgagattc tcggtgacct cgtggttcat cttctgatcg
     7501 aaccaggcga ccaggcggcg cacctcggcg cggtcgacgg ggttgatgcc gagcagcagc
     7561 ttgtcgcgat agacctcgtc gagatactcg gcgatggccg aggcctccgc cagcacggtg
     7621 ccgtcgggct cgatcagcac cggcacctcg cccgccgggt tgagcgccag gaactccggc
     7681 cggcgctccc aggtcttctc caccttcatg gtgaagtcga gcgccttttc ctgcagcac
//
LOCUS       DASYNS010000226        13106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1454677, whole genome shotgun sequence.
ACCESSION   DASYNS010000226 DASYNS010000000
VERSION     DASYNS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 13106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13106
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454677"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="VGP50_07060"
     CDS             <1..729
                     /locus_tag="VGP50_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV8027172.1"
                     /translation="VQNPGFFGQVRDFSRLAEACHRAGALLVVVVTEAVSLGLIEPPG
                     AMGADIVAGEGQALGNPLIFGGPHVGLFAVREKFIRQMPGRLAGETVDVEGRRGWVLT
                     LSTREQHIRREKATSNICTNSGLCALAFAIHLALLGEAGFTRLAALNHAQAVTLAERL
                     AKVPGVTLVNDSFFNEITLRLSRPAAPVVEALAERGVLAGVAASRFWPKRADMAELLI
                     VAATETNTEEDMDRLATGLREVLR"
     gene            726..2276
                     /gene="gcvPB"
                     /locus_tag="VGP50_07065"
     CDS             726..2276
                     /gene="gcvPB"
                     /locus_tag="VGP50_07065"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEV8027173.1"
                     /translation="MSEWSRALATPAAQPSPAGTLSGNRGLQIELPLLFERDAPGRSG
                     VDLPEPPAVGERLGGVRRRRPIGLPGLSEPQVVRHYTRLSQKNYAIDMGLYPLGSCTM
                     KHNPRLNEKLARLPGFADLHPLQPASTIQGALELIDALAHWLKVLTGMPAVALSPAAG
                     AHGELCGMMCIRAALDAKGEQGRRRVLVPESAHGTNPATAAACGFTVDPIAANERGRV
                     DLAALKEKLGPDVAAIMLTNPNTCGLFEDQMIEIADAVHRAGAYFYCDGANFNAIVGR
                     VRPADLGIDCMHINLHKTFSTPHGGGGPGAGPVVLAESLAAFAPLPWLVHDGKGFRLV
                     EQAEGEAGHALGRLKGFHGQMGMFVRALAYMMSHGADGLRQVSGDAVLNANYLLACLG
                     EDLSPSFPGPCMHEVLFDDRFLKETGVTTLDFAKALIDEGYHPMTMYFPLVVHGALLM
                     EPTETESKETLDRFIEVVKGLAARAKTDAAMFHAAPRFTPRRRLDETAAARKPMLRWR
                     PAPPEEAE"
     gene            complement(2387..2554)
                     /locus_tag="VGP50_07070"
     CDS             complement(2387..2554)
                     /locus_tag="VGP50_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027174.1"
                     /translation="MNDRRREPLLAQVLDDPLTQVLMLSDGVDRRELNHLIKEARQRI
                     ADAEGEPPSER"
     gene            2733..3623
                     /locus_tag="VGP50_07075"
     CDS             2733..3623
                     /locus_tag="VGP50_07075"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEV8027175.1"
                     /translation="MFYTPHRPRSQRGLAVDRAPPVRRLTDRSWRTHDLYHTLLIVPW
                     SGFLALIATGYVVFNLVFAALYLLQPGAIADAAPGSFADAFFFSVQTMATIGYGDMHP
                     QTLYANLLVTVEVLLAMTALALATGLIFARFSLPTARIMFSRVAVIAPYNGRPTLMFR
                     AANQRKNRILEAQVSLSILRDEVTAEGHHMRRFHDLPLTRGRTPMFALTWTVMHVIDE
                     TSPLWGTTPESLREEQAELVVTILGLDETVAQQIHARHSYLAEDIGWNRRFVDILGRL
                     EDGTRVVDYRRFHDVEDIAG"
     gene            3731..4831
                     /locus_tag="VGP50_07080"
     CDS             3731..4831
                     /locus_tag="VGP50_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV8027176.1"
                     /translation="MIARLLLSLIAATLAATVAARADDAKTLNIYNWSDYIAEDTVKR
                     FEAETGIKVNYDVYDSNEVLEAKLSAGHSGYDLVVPSAMPFLARQAEQGVYLKLDKSK
                     LGNYGNLDPQILAAAANADPGNQYGVPYLWGTTGIGYNPDKVKAALGADAPIDSLKLI
                     FDPANAKKLEGCGISLLDTPQEVFAAALAYVGRDPISRDPADLDRAAAVVMGIRPDVR
                     KFHSSQYINDLANGDLCVAFGYSGDVIQAKSRANDAKNGVTIAYSIPKEGAMMWIDMM
                     AIPRDAPHPENALKFINFILRPDIAAAISNTVAYANPNTLATKLVDAQIRDDPSIYPP
                     PAVRARLFFDKLVTPQYERTRTRDWTRVKTGS"
     gene            4938..6029
                     /locus_tag="VGP50_07085"
     CDS             4938..6029
                     /locus_tag="VGP50_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027177.1"
                     /translation="MSDAPYLRIENVVKRFAPGAAAAVDGVSLAVARGEFFALLGPSG
                     CGKTTLLRMLAGFERPDQGRILIDGADMADVPTYRRPVNMMFQSYALFPHMTAAQNVG
                     FGLKQDGLPRREIKERVAAMLALVKLGRLGDRKPHQLSGGERQRVALARALVQEPKLL
                     LLDEPLAALDRKLREDTRFELVRIQQRIGITFLLVTHDQEEAMTMASRIAVMSQGRIV
                     QLGTPQEVYEHPANRFVADFIGTVNLLDARVAGSEGGLLLLDSAEAGARLAVAHAPLA
                     AGTEATIAIRPEKIALAASPAGERNRLDGRIAAIAYRGDASTYEVELATGKILHAMAP
                     NTARHAAPFAVGQAVGLSFAPDAAVVLQS"
     gene            6026..6904
                     /locus_tag="VGP50_07090"
     CDS             6026..6904
                     /locus_tag="VGP50_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV8027178.1"
                     /translation="MKDERRAWAVLALPYAWLALFFLVPFAIVLKISLAEQAMGIPPY
                     TALLETGDGGTKLHASFANYLFLLSDSLYFRAYLNAVKFAGVSMVCCLLLGYPMAYGI
                     VRAPAAWRGFLLLMVILPFWTSFLIRVYAWIGLLKGNGLINNLLLSLGVVHEPLTLLN
                     NAFAIYLGITYSYLPFMVLPLYAALERLDPALLEAAADLGCRPWQAFLRVTLPLSLPG
                     IAAGSLLVFIPAVGEFVIPDLLGGPDTLMIGKVLWDEFFTNRDWPVASAVAIAMLLFL
                     LVPMGLVQRLLRGETA"
     gene            6901..7731
                     /locus_tag="VGP50_07095"
     CDS             6901..7731
                     /locus_tag="VGP50_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV8027179.1"
                     /translation="MINRLSPFLAIMLTLGYAFLYGPIASLVVYSFNASRLVTVWAGF
                     STRWYGELAQNEAIRAAALTSLEVAVSAASGALVLGTLAGFALQRYRRFKGRALFGLL
                     VTAPLVMPEVITGLSMLLLFVTLEQAIGWPAGRGVFTIIIAHVTLGTAFVAVIVQARL
                     AGFDRSLEEAALDLGARPWKVFLVITLPLIAPALMAGWLLAFTLSLDDLVIASFTSGP
                     SATTLPMVIFSSVRLGVSPQINALATLFLATVTVTVLLAGRLMGRSARGRSAARQKEG
                     "
     gene            complement(7927..8442)
                     /locus_tag="VGP50_07100"
     CDS             complement(7927..8442)
                     /locus_tag="VGP50_07100"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV8027180.1"
                     /translation="MDLKKHIRGVPDFPKPGILFYDISTLLAHGEAWQAAVRRLDEAL
                     RPHRPNLLVGIESRGFLVVAPLAYSLGCGFIMVRKKGKLPGPTIPLTYDLEYGSDTLE
                     IQADAIKPGARVIVLDDLLATGGTLGATIRLVRMVGGEVLAAACIIELAFLEGRKRID
                     VPFTSVVSYES"
     gene            8560..9705
                     /locus_tag="VGP50_07105"
     CDS             8560..9705
                     /locus_tag="VGP50_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HEV8027181.1"
                     /translation="MREDGGWVDIERRDKAVVLAAGGRWLVQNAAELDRSLNGLDSLG
                     DATAARIDLAGVEMLDTVGAWLVLRTRHRLQDRGIAATVENVPQAFAPLLEQVAKGGP
                     PAPPPQRPHGPREALRSALERLGKGTVDFFQTGRELLSFFGLICLTILRVLRQPERLR
                     GVSLIAQMERTGVTALPIVGLLSFLIGVVISYQGADQLRRFGAEIFTVNLLGIGILRE
                     LGVLLTAIIVAGRSGSAFTAEIGAMQVNEEIDALRTLGLDPIEVLVVPRLLGLVFVLP
                     LLTVYADFMSLLGGALMSWAALGIPLLQFIRQLNTAVSDATFFLGILKAPFFAAAIAL
                     VGCHSGLRVSRSAESVGRLTTVSVVHSIFLVIVFDAAFSILFARLHL"
     gene            9665..10498
                     /locus_tag="VGP50_07110"
     CDS             9665..10498
                     /locus_tag="VGP50_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027182.1"
                     /translation="MRHSRSSSRVSISEPLPAGAPVIRVRGLKTRVGGRTLHENLDLE
                     VYQGEVLGVVGGSGAGKSMLLRNIVGLDRPVAGEIEVFGEPVHSLNEERLRALESRWG
                     VLFQDGALFSSLTVAQNIAVPLKEHLALEGPLVDEIAAVKVALVGLPADAAAKFPAEL
                     SGGMRKRAGLARALALDPRLLFLDEPTAGLDPIAAGDFDALIRDLRQALGLTVFMVTH
                     DLDSLVAICDRIAVLVDKRLRIGTMAELLRDPHPWIQAYFHGPRGRAALAGRVPGQGD
                     G"
     gene            10501..11427
                     /locus_tag="VGP50_07115"
     CDS             10501..11427
                     /locus_tag="VGP50_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV8027183.1"
                     /translation="METRANYVAVGAFVLAMLVAIVVAVAWLGATSFNRDFAHYDIYF
                     EGSVTGLAQGSAVLYNGIQVGRVIEIRLNPKNLEQVRVTIEVDPTLQIKSDAAAVLQI
                     QGLTGGAFIDISGGSQSALALPRLDGDRYPVIASRQSGLQKVVTSAPEVLNRLIDVAD
                     RLAQIFDDKNRAALADTLQNVRQLTAVAAAHSGDIDGALTDAAVAAHELRGTAAELRQ
                     LFGQGDAANMFVTLSDTAKKLDHLADHIDGLVQENRPNLRDFSQNGLSQLSQLLVDAR
                     ALVANLNRITDEIERDPPRFLFGDRREGYQPR"
     gene            11424..12035
                     /locus_tag="VGP50_07120"
     CDS             11424..12035
                     /locus_tag="VGP50_07120"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEV8027184.1"
                     /translation="MSCASRRLVLAALALAPAGCAGLFPTGGAPPRLFTLTPARDFPA
                     GGRRVSWQLLVDVPASAAALDTDRVALSRSATSIDYFADSAWAERAPLMVQSLLVQSF
                     ENSSRITAVARESLGLRADYILQPELRHFEAQYAGGGAPVARVELGVKLVKMPERSIV
                     AGWHCETSAGAAQNQVPAIVDAFDSATRDALRQIVEWTLSAAG"
     gene            12087..12908
                     /locus_tag="VGP50_07125"
     CDS             12087..12908
                     /locus_tag="VGP50_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027185.1"
                     /translation="MGDLARLGRAELEAMAAAGEEALDCHRVLAKSGDGFLGEVLHGA
                     AASGDWTHYPPGDVYDPVTHAQYFYHTHPPAERRPREEGHFHTFLRPRGMAPGTRPLV
                     LPELAIADAPAAPADPATPPAPQPNQGADNDEPSHLIAIAMDGNGNPVRLFTTNRWVT
                     GETWYAAADVMAMLDRFVIDLARPSWPLDRWLGALFRLFKPQMRALIQARDDAVMSWR
                     RRHRGKVHVFEDRRLDVTSALDIDVEEQIRDIGLALSGSKGQAPGRPTFFLAPGS"
BASE COUNT         1924 a         4612 c         4307 g         2263 t
ORIGIN      
        1 gtgcagaatc ccggcttctt cgggcaggtg cgcgatttct cgcgcctcgc cgaggcctgc
       61 catcgcgcgg gcgcgctgct cgtcgtggtc gtcaccgagg ccgtgtcgct gggcctgatc
      121 gagccgccgg gcgccatggg cgccgacatc gtcgcgggcg aaggccaggc gctgggcaac
      181 ccgctgatct tcggcggccc gcatgtcggt ctcttcgccg tgcgcgagaa gttcatccgc
      241 cagatgccgg gccggctcgc cggcgagacc gtcgacgtcg agggacggcg cggctgggtg
      301 ctgacgctct cgacgcgcga gcagcacatc cggcgcgaga aggcgacgag caacatctgc
      361 accaattccg gcctctgcgc gctcgccttt gccattcacc tggcgctcct gggcgaagcc
      421 ggcttcacgc ggctcgccgc gctcaatcat gcgcaagcgg tgacgctggc cgagcggctc
      481 gccaaggtgc cgggcgtgac gctggtcaat gacagcttct tcaacgagat cacgctccgc
      541 ctgtcgcggc cggcggcgcc ggtcgtcgag gcgctggcag agcggggcgt gctggcgggc
      601 gtggccgcga gccgcttctg gccgaagcgc gccgacatgg cggagctgct gatcgtcgcc
      661 gctaccgaga cgaataccga ggaagacatg gatcgcctcg ccacgggctt gcgcgaggtg
      721 ctgcggtgag cgagtggagc cgggcgctgg cgacgccggc ggcgcagccg agcccggccg
      781 ggacgctcag cggcaatcgc gggctgcaga tcgagctgcc cttgctcttc gagcgcgatg
      841 cgccgggacg ctccggcgtc gatctgcccg agccgccggc cgtcggcgag cgtctgggcg
      901 gcgtgcgccg gcggcggccg atcgggcttc ccggcctcag cgagccgcag gtcgtgcggc
      961 actacacgcg gctgtcgcag aagaactacg cgatcgacat gggcctttat ccgctcggct
     1021 cctgcacgat gaagcacaat ccgcgcctca acgaaaagct ggcgcggctg ccgggcttcg
     1081 ccgatctcca cccgctgcag ccggcatcga cgatccaggg cgcgctcgag ctgatcgatg
     1141 cgctggcgca ttggctgaag gtgctgacgg gcatgccggc ggtggcgctg agcccggcgg
     1201 ccggggccca tggcgagctg tgcggcatga tgtgcatccg cgccgcgctc gacgccaagg
     1261 gcgagcaggg ccgccgccgc gtgctggtgc cggaatcggc gcatgggacg aatccggcca
     1321 cggccgcggc ctgtggcttc accgtcgacc cgatcgccgc caatgagcgc ggccgcgtcg
     1381 atctcgcagc gctcaaggag aagctcgggc ccgatgtcgc ggcgatcatg ctgacgaacc
     1441 cgaacacctg cggcctgttc gaggatcaga tgatcgagat cgccgacgcc gtgcatcgcg
     1501 ccggcgccta tttctactgc gacggcgcca atttcaacgc catcgtcggc cgcgtgcggc
     1561 cggccgatct cggcatcgac tgcatgcaca tcaacctgca caagaccttc tcgacgcccc
     1621 atggcggcgg cgggcccggt gccgggccgg tggtgctggc cgaaagcctc gccgcctttg
     1681 cgccgctgcc ctggctcgtc catgacggca agggcttccg gctggtcgag caggcggagg
     1741 gcgaggccgg ccacgcgctg ggccgcctca agggctttca cggccagatg ggcatgttcg
     1801 tccgcgcgct tgcctacatg atgagccatg gcgccgacgg gctgcgccag gtctcgggcg
     1861 atgccgtgct caacgccaat tacctgctgg catgcctcgg cgaggacctc tcgccgtcct
     1921 tccccgggcc ttgcatgcat gaggtgctgt tcgacgaccg tttcctcaaa gagaccggcg
     1981 tcaccaccct cgatttcgcc aaggcgctga tcgacgaggg ctatcacccc atgacgatgt
     2041 attttccgct ggtcgtccac ggcgcgttgc tgatggagcc gacggagacc gagagcaagg
     2101 agacgctcga ccgcttcatc gaggtggtga agggcctggc ggcgcgggcc aagacggatg
     2161 ccgcgatgtt ccacgccgcg ccgcgcttca cgccgcggcg ccgcctcgac gaaaccgcgg
     2221 ccgcccggaa gcccatgctg cgctggcgcc cggcaccgcc ggaggaggcg gagtaggctc
     2281 tcgctgcagt tgcctcatac gtccggcgga cctctccctt tccgcccttc agggcggaga
     2341 gggaggtcac actctcatgt gagaatcaac agccctctgc ggcgcgctag cgttcgctcg
     2401 gtggctcgcc ctcggcgtcg gcaatgcgct ggcgcgcttc cttgatgagg tggttcagct
     2461 cgcgccggtc gacgccgtcg ctcagcatca gcacctgggt caggggatcg tcgaggacct
     2521 gagcgagcag gggctcgcgg cggcgatcgt tcatggggtc ctccctgggc tgaagccgcg
     2581 tcgttgggcc gacgcggacg actcctgccg ataatacgcg ctcaaggagc tatctattcc
     2641 cgcggcgctg tgagttttta gggacgtggc agcaacgcca cagatgattc gaccgctcgc
     2701 acgctcggat attattccag taggataccc gcatgttcta cacgccccac cgacctcgct
     2761 cgcaacgcgg cctggccgtc gaccgcgccc cgcccgtgcg ccgcctcacc gaccgctcct
     2821 ggcgcacgca cgacctctat cacaccttgc tgatcgtgcc gtggtcgggt ttcctggcgc
     2881 tgatcgcgac cggctatgtc gtcttcaacc tggtcttcgc cgcgctctac ctgttgcagc
     2941 cgggggcgat cgccgatgcc gcgccggggt ctttcgccga tgccttcttc ttcagcgtcc
     3001 agaccatggc cacgatcggc tatggcgaca tgcatccgca aacgctctat gccaatctgc
     3061 tggtcaccgt ggaggtgctg ctggcgatga cggcgctcgc cctcgccacc ggtctcatct
     3121 tcgcccgctt ctctctcccc accgcccgca tcatgttcag ccgcgtcgcg gtgatcgctc
     3181 cctataatgg ccggccgacc ctcatgttcc gcgccgccaa ccagcgcaag aaccggatcc
     3241 tcgaggcgca ggtctcgctc tcgatcctgc gcgacgaagt gaccgcagag gggcaccaca
     3301 tgcgccgctt ccatgacctg ccgctgaccc gtggccggac gccgatgttc gctctgacct
     3361 ggacggtgat gcatgtcatc gacgagacca gcccgctctg gggcaccacg cccgagtcgc
     3421 tgcgcgagga gcaggcagag ctggtggtga cgatcctcgg gctcgacgag accgtcgcgc
     3481 agcagatcca tgcgcgccac tcctatctcg ccgaggacat cgggtggaac cggcgcttcg
     3541 tcgacatcct cgggcgcctc gaggatggga cgcgcgtggt cgactatcgc cgcttccatg
     3601 acgtggagga catcgcgggc tgagcgcgcg acgatccccg ggtctgctgc ggaggcgccc
     3661 cgacgattga caagcgctga tgagcctcct tgaatgatgc ccgtgttcgg gtgctttctt
     3721 gggggattgg atgatcgctc gcctgcttct gtcgctgatt gccgcgaccc ttgcagcaac
     3781 ggtcgccgcc cgcgccgacg acgccaaaac cctcaacatc tacaactggt ccgactatat
     3841 cgccgaggat acggtcaagc gcttcgaggc ggagaccggc atcaaggtca attatgacgt
     3901 ctatgacagc aacgaggttc tcgaggccaa attgtcggca ggccattcgg gctacgacct
     3961 ggtcgtgccc tcggccatgc cgttcctcgc gcgtcaggcc gagcaaggcg tctatctgaa
     4021 gctcgacaag tcgaagctcg gcaattacgg caacctcgat ccgcagatcc tcgccgccgc
     4081 cgccaatgcc gatcccggca atcaatatgg cgtgccttac ctctggggca cgacgggcat
     4141 cggctacaac ccggacaagg tcaaggcggc gctgggcgcc gatgcgccga tcgacagcct
     4201 gaagctgatc ttcgatccgg ccaatgccaa gaagctcgag ggctgcggca tctcgctgct
     4261 cgacacgccg caggaagtat tcgcggcggc gctcgcctat gtcggccgcg atcccatcag
     4321 ccgcgatccc gccgacctcg atcgcgcggc cgcggtggtg atggggatcc gcccggacgt
     4381 gcgcaagttc cactcctcgc aatacatcaa cgatctcgcc aatggcgacc tctgcgtcgc
     4441 cttcggctat tcgggcgacg tcatccaggc gaagagccgc gccaacgacg ccaagaacgg
     4501 cgtcaccatc gcctacagca tccccaagga aggggcgatg atgtggatcg acatgatggc
     4561 catccccagg gatgcgccgc atcccgagaa tgccctcaaa ttcatcaatt tcatcctgcg
     4621 gcccgacatc gccgcggcga tcagcaacac cgtcgcctac gccaatccca acacgctcgc
     4681 caccaagctc gtcgacgcgc agatccgcga cgatcccagc atctatccgc cgccggcggt
     4741 gcgcgcgcgg ctctttttcg acaagctggt gacgccgcaa tacgagcgga cgcgcacccg
     4801 cgactggacc cgggtcaaga cgggaagcta gcgccagcaa tgcccgcgcg cggacccctc
     4861 actttcccgg cgctccgcgc cgggcccctc cctctcccac aaggggagag ggatagcgtt
     4921 gccgcgccct cggggtcgtg agcgacgctc cttatctgcg catcgagaat gtggtgaagc
     4981 ggttcgcgcc aggggcggcg gccgcggtgg atggcgtctc gctggcggtg gcgcggggcg
     5041 agttcttcgc gctgctcggg ccttcgggct gtggcaagac cacgttgctg cgcatgctcg
     5101 ccggtttcga gcggcccgat cagggccgca tcctcatcga cggcgccgac atggcggatg
     5161 tgccgaccta tcgccgcccc gtaaacatga tgttccagtc ctatgcgctc tttccgcaca
     5221 tgacggcggc gcagaatgtc ggcttcggcc tgaagcagga cgggctgccg cggcgcgaga
     5281 tcaaggagcg cgtcgcggcg atgctcgctc tcgtcaagct gggccgtctc ggcgaccgca
     5341 agccgcatca gctctcgggc ggcgagcgcc agcgcgtggc gctcgcccgg gcgctcgtcc
     5401 aggagccgaa gctgctgctg ctcgacgagc cgctggcggc gctcgaccgc aagctgcgcg
     5461 aggatacgcg cttcgagctc gtccgcattc agcagcgcat cggcatcacc tttctcctgg
     5521 tgacccatga ccaggaggaa gccatgacca tggcctcgcg gatcgccgta atgtcgcaag
     5581 ggcgcatcgt ccagctcggc acgccgcaag aggtctacga gcatccggcg aaccgtttcg
     5641 tcgccgactt catcggcacc gtcaatctgc tcgacgcccg cgtcgccggc agcgaaggcg
     5701 gcttgctttt gctcgacagc gccgaggccg gcgcgcggct cgccgtcgcc catgcgccgc
     5761 tggccgcggg caccgaggcg accatcgcca tccgtccgga gaagatcgcg ctcgcggcgt
     5821 cgcccgctgg cgagcgcaac cgcctcgacg gccggatcgc cgccatcgcc tatcgcggcg
     5881 acgcctcgac ctacgaggtc gagctcgcga cgggcaagat cctgcacgcc atggcgccca
     5941 acaccgcgcg ccatgcggcg cccttcgcgg tcgggcaggc cgtcggcctc tccttcgcgc
     6001 cggacgccgc cgtggtcctc caatcatgaa ggatgagcgc cgcgcctggg cggttctggc
     6061 gctgccttac gcctggcttg cgctcttctt cctggtgcct ttcgcgatcg tgctcaagat
     6121 cagcctcgcc gagcaggcga tgggcattcc cccttacacg gcgctcctcg agaccggcga
     6181 cggcggcacg aagctccatg cgagcttcgc caattatctc ttcctgctca gcgacagcct
     6241 ctatttccgg gcctatctca acgccgtcaa attcgccggc gtctcgatgg tctgctgcct
     6301 gctgctgggc tacccgatgg cctatggcat cgtgcgcgcg cccgccgcct ggcgcggctt
     6361 cctgctgctg atggtgatcc tgccgttctg gacgagtttt ttgatccgcg tctatgcctg
     6421 gatcggcctc ctcaaaggca acggcctcat caacaacctg ctgctgtcgc tgggcgtcgt
     6481 ccacgagccg ctgacgctgc tcaacaatgc tttcgcgatc tatctcggca tcacttattc
     6541 ttatctgccg ttcatggtgc tgccgctcta tgccgcgctc gagcggctcg acccggcgct
     6601 tctcgaagcg gcggcggatc tgggctgccg tccctggcag gccttcttgc gcgtcaccct
     6661 gccgctttcg ctgccgggca tcgccgccgg ctcgctcctc gtcttcatcc cggccgtggg
     6721 cgagttcgtg atccccgatc tcctgggcgg gcccgatacg ctgatgatcg gcaaggtgct
     6781 gtgggacgag ttcttcacca accgcgactg gcccgtcgcc tcggcggtgg ccatcgccat
     6841 gctgctgttc ctgctcgtgc ccatggggct ggtgcagcgc ctgttgcgcg gcgagacggc
     6901 atgatcaacc gtctctcgcc cttcctcgcg atcatgctga cgctgggtta tgcgtttctc
     6961 tacgggccga tcgcgtcgct cgtcgtctat tccttcaatg cgtcgcgcct cgtcaccgtc
     7021 tgggccgggt tctcgacgcg ctggtatggc gaactcgcgc agaatgaagc gatccgcgcc
     7081 gccgcgctca ccagcctcga agtcgcggtg tccgccgcga gcggcgcgct cgtcctcggc
     7141 acgctcgcgg gcttcgcgct ccagcgctat cgccgcttca agggccgcgc gctcttcggt
     7201 ctcctggtga cggcgccgct ggtcatgccc gaggtgatca cggggctctc gatgctcctg
     7261 ctcttcgtga cgctggagca ggcgatcggc tggcccgccg ggcgcggcgt cttcaccatc
     7321 atcatcgccc atgtgacgct gggcacggcc ttcgtcgccg tcatcgtgca ggcgcggctc
     7381 gcgggcttcg accgctccct cgaggaagcg gcgctcgatc tgggcgcgcg tccttggaag
     7441 gtgttcctgg tcattacttt gccgctgatc gcgccggcgc tgatggccgg ctggctgctc
     7501 gccttcacgc tttcgctcga cgacctcgtc atcgccagct tcacctcggg gccgtccgcg
     7561 acgacgctgc cgatggtgat cttctcaagc gtcaggctgg gcgtcagccc gcagatcaac
     7621 gcgctggcga cgctcttcct cgccacggtc acggtgacgg tgctgctcgc cggacgcttg
     7681 atggggcgct cggcgcgagg ccgatcggcg gcgcgtcaga aagaaggttg accacagagg
     7741 cacagagaca cagaggaaga ggaggaggaa agaaagattc accgatgagg cagtgagaag
     7801 acttcttctg gcgcgcgaag cgcgcccttg tctctcttct cactgcctca ttgtctctgt
     7861 ggtcaatctt cttccttcct ctgtgtctct gtgcctctgt ggtcaatctc cttcgggccc
     7921 cgcccgtcag gactcgtagc tcaccacgga ggtgaagggg acgtcgatgc gcttccggcc
     7981 ttcgaggaag gcgagctcga tgatgcaggc ggcggccagc acctcgccgc cgaccatgcg
     8041 cacgagacgg atggtggcgc cgagcgtgcc gcccgtcgcc agcaggtcgt cgagcacaat
     8101 cacccgggcg ccgggcttga tcgcatcggc ctggatctcg agcgtgtcgc tgccgtattc
     8161 gaggtcatag gtgagaggaa tggtcgggcc cggcagcttg cccttctttc gcaccatgat
     8221 gaagccgcag cccaggctgt aggcgagcgg cgcgacgacg aggaagccgc gcgattcgat
     8281 gccgaccagc aaattcggcc gatgggggcg gagcgcctcg tcgagccggc gcacggcggc
     8341 ctgccacgct tcgccatgcg ccagcaacgt cgagatgtcg tagaaaagga ttcccggctt
     8401 cgggaaatcc ggaacccccc ggatgtgctt cttgaggtcc atccaccact ccttcgccaa
     8461 tcttgggcga ccgctactct agcgaagtcg gtggccgccg acagcggctt tttggaccgg
     8521 cctctcgtca ccagaaggcc atcgtgtaga ctggccgcca tgcgagagga cggcggctgg
     8581 gtggacatcg agcggcggga caaggccgtc gtcctggcgg cgggcggccg ctggctggtc
     8641 cagaacgccg ccgagctcga tcgcagcctg aatgggctcg acagcctcgg cgatgccact
     8701 gccgcgcgca tcgatctggc gggcgtcgag atgctcgata ccgtcggcgc ctggctcgtg
     8761 ctgcgcacgc gccaccggct ccaggaccgc ggcatcgccg ccaccgtcga gaatgtgccg
     8821 caagcctttg cgccgctcct cgagcaggtg gcgaagggcg ggccgccggc gccgccgccg
     8881 cagcgccctc atggcccgcg cgaagcgctg cggtcggcgc tcgagcgcct gggtaaaggc
     8941 acggtcgact tcttccagac cggccgcgag cttctcagct tcttcggcct gatctgcctc
     9001 accatcctgc gcgtgctgcg ccagccggaa cggctccggg gggtgtcgct gatcgcgcag
     9061 atggagcgca ccggagtgac ggcgctgccg atcgtcgggc tgctctcttt cctgatcggc
     9121 gtcgtcatct cctatcaggg cgccgaccag ttgcggcgct tcggcgcgga gatcttcacg
     9181 gtgaacctgc tcggcatcgg catcctgcgc gagctgggcg tgctcctcac cgccatcatc
     9241 gtcgcgggac gctcgggcag cgcattcacc gccgagatcg gcgcgatgca ggtcaacgag
     9301 gagatcgacg cgctaaggac gctcgggctc gatcccatcg aggtgctggt ggtgccgcgc
     9361 ctcctcgggc tcgtcttcgt gctgccgctg ctcaccgtct acgccgattt catgtcattg
     9421 ctaggcggcg cgctcatgtc ctgggccgcg ctcggcatcc cgcttctcca gttcatccgc
     9481 cagctcaaca cggccgtctc cgacgccacc ttctttctcg gcatcctgaa ggcgccgttc
     9541 ttcgccgcgg ccatcgcgct ggtcggctgc cattcggggc tccgcgtgtc gcgcagcgcc
     9601 gagagcgtcg gccggctcac tacggtctcc gtcgtccatt cgattttcct ggtcatcgtc
     9661 ttcgatgcgg cattctcgat cctcttcgcg cgtctccatc tctgagccgc tgccggcggg
     9721 cgcgccggtg atccgcgtgc gcgggctcaa gacccgcgtc ggcgggcgaa cgctgcatga
     9781 gaatctcgac ctcgaggtct atcagggcga ggtgctgggc gtcgtcggcg gctcgggggc
     9841 cggcaagtcg atgctgctcc ggaacatcgt cggcctcgat cggccggtgg cgggcgagat
     9901 cgaggtcttc ggcgagcccg tccattcgct caacgaggag cggctgcgcg ccctcgagtc
     9961 gcgttggggc gtgctgttcc aggacggcgc gctcttcagc tcgctcaccg tcgcgcagaa
    10021 catcgccgtg ccgctcaaag agcatctcgc gctcgagggg ccgctcgtcg acgagatcgc
    10081 cgccgtcaag gtcgccctgg tcggcttgcc ggccgatgcg gcggcgaaat tcccggccga
    10141 gctctcgggc ggcatgcgca agcgcgccgg cctggcgcgc gcgctggcgc tcgatccgcg
    10201 gctcctcttc ctcgacgagc cgaccgccgg gctcgacccc atcgccgcgg gcgacttcga
    10261 tgcgctcatc cgcgacctcc gccaagccct gggcctcacc gtcttcatgg tgacgcatga
    10321 cctcgacagc ctggtggcga tctgcgaccg catcgccgtg ctggtcgaca agcgattgag
    10381 aatcggcaca atggcggaac tgttgcgcga tccgcacccg tggatccagg cctatttcca
    10441 cggaccgcgt gggcgcgcgg cgctggcggg aagggttcca ggacaaggcg acggataacg
    10501 atggaaacgc gcgcgaatta tgtggcggtc ggggctttcg ttctggcgat gctggtcgcc
    10561 atcgtggtcg ccgtcgcctg gctgggcgcg accagcttca atcgcgattt cgcgcattac
    10621 gacatctatt tcgagggctc ggtcaccggc ctcgcccagg gctcggcggt gctctacaac
    10681 ggcatccagg tcggccgcgt catcgagatc cgcctcaatc ccaagaatct cgagcaggtg
    10741 cgagtcacca tcgaggtcga tccgacgctc cagatcaagt cggacgccgc cgccgttctc
    10801 cagatccagg gcttgaccgg cggcgccttc atcgacatca gcggcggcag ccagagcgcg
    10861 ctggcgctcc cgcgcctgga cggcgatcgc tatccggtga tcgcatcgcg gcaatcgggg
    10921 ctgcagaagg tggtgacgag cgcgcccgag gtgctcaacc gcctgatcga cgtcgccgac
    10981 cggctcgcgc agatcttcga cgacaagaac cgcgcggcgc tcgccgacac gctgcagaat
    11041 gtgcggcagc tgacggcggt cgccgccgcg cactccggcg acatcgacgg cgcgctcacg
    11101 gatgcagcgg tcgcggccca tgagctgcgc ggcaccgcgg cggagctgcg ccagctcttc
    11161 gggcagggcg acgcggccaa catgttcgtg acgctctccg acacggcgaa gaagctcgat
    11221 cacctcgccg atcacatcga tggcctggtg caggagaatc gcccgaacct gcgcgacttc
    11281 agccagaacg gcctgagcca gctgagccag ctgctcgtcg atgcgcgcgc gctcgtcgcc
    11341 aatctcaacc gcatcaccga cgagatcgag cgcgatcccc cgcgcttcct cttcggcgac
    11401 cgccgcgagg gctaccagcc gcgatgagct gcgcctcgcg tcgtctcgtg ctcgcggcct
    11461 tggcgctcgc gcccgccggc tgcgccggtc tctttccgac cggcggcgcg ccgccgcggc
    11521 tcttcaccct gacgccggcg cgcgactttc ccgccggcgg gaggcgcgtt tcctggcagc
    11581 tgctggtcga tgtgccggcg agcgccgccg cgctcgacac cgatcgcgtc gccttgagcc
    11641 ggagcgcgac cagcatcgac tacttcgccg attccgcctg ggccgagcgc gcgccgctca
    11701 tggtgcagtc gctgctggtg cagtctttcg agaattccag ccggatcacg gcggtcgcgc
    11761 gcgaatcgct gggcttgcgt gccgactaca tcctgcagcc cgaactgcgc catttcgagg
    11821 cgcagtatgc cggcggcggc gcgcccgtcg cccgggtcga gctcggcgtc aagctggtga
    11881 agatgccgga gcgcagcatc gtcgcggggt ggcactgcga gacgagcgcc ggggccgccc
    11941 agaatcaagt gccggcgatc gtcgacgcct tcgactccgc gacgcgcgac gcgcttcggc
    12001 agatcgtcga atggacgctc tccgccgccg gttgacgctt gccgccgccg ccacgcgccg
    12061 acataactga ggcaggaagg tcgcgcatgg gggatcttgc ccgcctggga cgtgccgagc
    12121 tcgaagccat ggcggcggcg ggcgaggagg cgctcgattg ccaccgggtc ctggcgaaga
    12181 gcggggacgg cttcctcggc gaggtgctgc acggcgcggc cgcttccggc gactggacgc
    12241 attatccgcc cggcgacgtc tacgatcccg tgacccacgc gcagtatttc tatcacacgc
    12301 atccgccggc cgagcgccgg ccgcgcgagg aagggcactt ccacaccttc ctgcgcccgc
    12361 ggggcatggc tccggggacg cggcccctcg tgctgccgga actcgccatc gccgatgcgc
    12421 cggcggcgcc ggccgatccg gcgaccccgc ccgcgccgca gcccaaccag ggcgccgaca
    12481 atgacgagcc gagccacctc atcgccatcg ccatggacgg caacggcaat ccggtgcggc
    12541 tcttcaccac gaaccgatgg gtcacgggcg agacctggta cgcggccgcc gacgtcatgg
    12601 cgatgctcga ccgcttcgtg atcgacctgg cccggccgtc ctggccgctc gaccggtggc
    12661 tcggcgccct gttccgcctg ttcaagccgc agatgcgcgc gctgatccag gcccgcgacg
    12721 atgccgtcat gtcctggcgc cgccgccatc gcggcaaggt gcatgtcttc gaagaccggc
    12781 gcctcgacgt cacctccgcc ctcgacatcg atgtcgaaga gcagatccgc gacatcgggc
    12841 tcgccctgtc cgggagcaaa ggccaggcgc cgggacggcc gacctttttc ctggctccgg
    12901 gctcctgacc gcggcttcct ggttaagcga acattaaatc ttggcggaca tcatggttaa
    12961 tcgcagatgc tccgcgaggt cgcccgccaa agatgaccag taccctctcg aaatccgacg
    13021 ttgcccggct gctcacggat tcttcgcccg agacaagggc ggatgtggcg gcaaaggtgg
    13081 cgcgcgaggt ggatggaagc gcgctc
//
LOCUS       DASYNS010000227         4542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3951613, whole genome shotgun sequence.
ACCESSION   DASYNS010000227 DASYNS010000000
VERSION     DASYNS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3951613"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VGP50_07130"
     CDS             <1..323
                     /locus_tag="VGP50_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV8027186.1"
                     /translation="EEIGTSVLVLAEIRHGIELKRRNDPEQAKSLDRWFSRMRIGLGD
                     RVLPIDEPVAEAWALLGIPDPLPLIDGLLAATAKVHGLTLVTRNVADIAVVGVSLLDP
                     FSVG"
     gene            511..924
                     /locus_tag="VGP50_07135"
     CDS             511..924
                     /locus_tag="VGP50_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027187.1"
                     /translation="MRLPMRPLLTLIVSALTICTLAPRAMPALAADTEAQDRDAVQNA
                     AGSGRLHEDPERHALRQKVLNAANRVDASPCDTQQRQKLSEAFDAYQHHIEATADDRV
                     ETLTLGDGRVLDVSRHFDDPVEDAWRFALTTPCSN"
     gene            921..1493
                     /locus_tag="VGP50_07140"
     CDS             921..1493
                     /locus_tag="VGP50_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027188.1"
                     /translation="MSTAAIRLWLSALVAVVVIGAAVLPRLAELPHSADAPSTPSPEP
                     ELWSFERAVVNRESLDVARARGQVHEDPALRKLRAAVLAASSRLEFSPCDNQLRPPLR
                     QAIGALLVALREAAGEKRETATIDGADFDATPFLNAAAAAVIAEARQVGLVYREDLPA
                     EVGVLFPPRPGDPDSGRYGGRFACVDGGRG"
     gene            1796..2623
                     /locus_tag="VGP50_07145"
     CDS             1796..2623
                     /locus_tag="VGP50_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027189.1"
                     /translation="MSFALEFYSLSWAALRSALSQRQPELIAALRDRQWTPLLEDCDL
                     GQKAPHGFLHAARLALFRRQGEEQAPWENVDAVIAGGLDEIAEAMSRGTTGRALIEVS
                     EGAALVVAAMVRQMGKRLGAIRHDGTARRDRGLPIDFRTMFLDGIAGSCFSDHRLGEN
                     LASRPLFGLMHLDFLSWGGLSRNEIDTLLRKYSLGQEQWQDEEWQVIAPDAEAWLDEL
                     VTSLRDAAAAKSDLVTLYVTVQKHFTSFGDALEDELVEDFAECGESLSDHFAPENGA"
     gene            2641..3600
                     /locus_tag="VGP50_07150"
     CDS             2641..3600
                     /locus_tag="VGP50_07150"
                     /inference="COORDINATES: protein motif:HMM:NF023203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027190.1"
                     /translation="MAKAQEPQPSDVFTLARLRAALLKLTGTDDALPESAAEIAPAES
                     APAAAPARTDYAVYTREFDEVVRPEQMATPAELSALHAELVAEIERLALTHDGWIRPW
                     AKKIAHRAGERTLLVTLLLDNSGSLRGQQIRPIAAWAALVGDILRHSGVLVEVLGFTT
                     AEWRGGRARQRWVSDGSPERPGRLNDLRYIVYQAHDDDAYCAANFALMLKDGVLKENI
                     DGEALLWAADRQRGIAADEKIIIIMSDGAPVDDSTAAANHPDYLHDHLVEATRLIEGD
                     RQSTLYGIYLEGDYRPFAYYTRSATIRAPEQMGLAVLRFLATG"
     gene            complement(3779..4210)
                     /locus_tag="VGP50_07155"
     CDS             complement(3779..4210)
                     /locus_tag="VGP50_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV8027191.1"
                     /translation="MTGIFDALAAGDVIDGAAFAVSRETIRDFCDASLDYNPLHLDDA
                     YMAGSFGKTNFGGVIMHGMTNFALITRMLTDWAYAKGAVHRRLETRWLKPARPGDTLR
                     PQGRLKAKQVTSRSRWVTIDIEMRNQRDEIVATGEAMVEFP"
     gene            complement(4219..>4542)
                     /locus_tag="VGP50_07160"
     CDS             complement(4219..>4542)
                     /locus_tag="VGP50_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV8027192.1"
                     /translation="LHPLYFDEDYARRSVHGGLIAPPAIHILLMFACTPADDWMRSPG
                     TVNAGQSWSYNLPARPGDTITLEARALDKFIRKERLFVVHDNVFFNQHGAVICSGRGW
                     TIRPM"
BASE COUNT          736 a         1494 c         1570 g          742 t
ORIGIN      
        1 tcgaggagat cgggacgagc gtcttggtgc tcgccgaaat ccgtcatggc atcgagttga
       61 aacgacggaa cgacccggaa caggctaaat ctctcgatcg atggttctcg cgaatgcgga
      121 tcgggctcgg agatcgcgtc ctgcctatcg atgagccggt cgcggaagcc tgggcgctct
      181 tgggaattcc cgatccattg ccgctgatcg acgggctcct ggcggccaca gccaaagtgc
      241 atggcctgac gctggtgacc cgaaatgtcg cagacattgc tgtcgtcggc gtgtcgctgc
      301 tcgatccatt ttccgtcggc tgacaggtcg aggagcaaga acgccgcgcg aatactcgcg
      361 ccgatcgcgc ccgtttccgg gaagataatt gtcgtcgaac gcggccaaac cggcgcgggg
      421 cgggcgcgtg agcgcctgag cgcctgagca ttagaaagtc ctcaacgacc gacatgcgac
      481 actgcggctt cgcgggaatg atctattccg atgaggctgc cgatgcgccc tttgctcacg
      541 ttaatcgtca gtgccttaac catctgcaca ctggcgccgc gcgcgatgcc cgcgctcgcg
      601 gccgacaccg aggcgcagga tcgcgatgcc gtgcagaacg ccgccggcag cggcaggctg
      661 catgaggatc cggagcgcca tgcgctgcgc cagaaggtgc tgaacgcagc caatcgcgtc
      721 gacgcgtcgc cctgcgacac ccagcagcgc caaaagctga gcgaggcgtt cgacgcctat
      781 cagcatcaca tagaggcgac ggcagacgac cgggtcgaga cgttgaccct cggagacggt
      841 cgcgtgctcg atgtgtcgcg gcacttcgac gatccggtcg aggatgcctg gcgcttcgcg
      901 ctcacgacgc cctgcagcaa ttgagcaccg cggcgatacg cctctggctg tcggcgctcg
      961 tggccgtcgt ggtcatcggc gccgcggtgc tgccgcgcct cgcggagttg ccgcattcgg
     1021 ccgacgcgcc gtcgacaccg tcgcccgagc ccgagctctg gagcttcgaa cgcgcggtgg
     1081 tgaatcgcga gagcctggac gtggcgcgcg cgagagggca ggtgcacgag gacccggcgc
     1141 tgcgcaagct gcgcgccgcc gttcttgccg cgtcgtcgcg cctcgaattc tcgccttgcg
     1201 acaatcaact gcggccgccg ctgcggcagg cgatcggcgc gctgctggtc gcgctgcgcg
     1261 aggcggcggg agagaagcgc gagaccgcga cgatcgacgg cgccgacttc gatgcgacgc
     1321 cgttcctcaa cgccgcggct gcggctgtga tcgccgaagc gcggcaggtc gggctcgtct
     1381 atcgcgagga tcttccggcg gaggtcggtg tgctctttcc gccgcggccg ggcgatccgg
     1441 atagcgggcg atatggcgga cggtttgcgt gcgtcgatgg cgggcggggg tagggccgcg
     1501 gcagcggttt cgcggagagg cacctcaccc tcccactgct tcgctgacgc ttcgcagtgg
     1561 gcccctccct ctccccgctt cgcgcggaga gggataatga cgtcctgctc tgtgcgggct
     1621 ttcgcggttg caagagcaac cgctggcgcc cgcccgcgac gacgcttcgc gtcgcgcgag
     1681 cgtcgcgaga gtgcactgca cggttaatcc gaggttaacc ggcggcactt agcccctctt
     1741 taacataagc gatgttgaat cggctcctcc gagggacgca ttcgaggaga tgccggtgag
     1801 cttcgcactc gagttctaca gcttgtcctg ggcggcgctc agatcggcgc tcagccaacg
     1861 gcagcccgag ctcatcgcgg cgctgcgcga ccggcaatgg acgccgctcc tcgaggattg
     1921 cgatctcggc cagaaggcgc cgcacgggtt tctccatgcc gcgcgcctcg cgctgtttcg
     1981 ccgccagggc gaggagcaag cgccctggga gaatgtcgat gcggtcatcg cgggcggcct
     2041 cgacgagatc gcggaagcga tgtcgcgcgg cacgacggga cgcgcgctca tcgaggtgag
     2101 cgagggcgcg gcgctggtcg tcgccgcgat ggtccggcaa atgggcaagc gcctgggcgc
     2161 gatccgtcac gatggcacgg cccggcgcga ccgcggcctc cccatcgatt tccgcacgat
     2221 gttcctcgac ggcatcgccg gctcctgctt cagcgatcat cgcctcggcg agaatctcgc
     2281 ttcacgcccg ctcttcggcc tcatgcatct cgatttcctc agctggggcg gcctgtcccg
     2341 caacgagatc gacacgctgc tgcgcaaata ttcgcttggg caggagcagt ggcaagacga
     2401 ggagtggcag gtgatcgccc ccgatgccga ggcgtggctc gacgagctgg tgacgtcgct
     2461 gcgcgacgca gcggcggcga aaagcgatct cgtcacgctc tacgtgaccg tgcaaaagca
     2521 tttcacctcc ttcggcgacg cgctcgagga cgagctggtg gaggatttcg ccgagtgcgg
     2581 cgagagcctc tcggaccact tcgcccctga gaacggcgcc taggcgccgg gagtacgatc
     2641 gtggcaaagg cgcaagagcc gcagccctcc gatgtcttca ccctggcgcg gctgcgtgcg
     2701 gcgctgctga agctgaccgg cacggacgac gcgctgcccg aaagcgcagc ggaaatcgcg
     2761 cccgccgaga gcgcgccggc ggcggcgccg gcgcggaccg attacgccgt ctacaccaga
     2821 gaattcgacg aggtggtgcg gcccgagcag atggcgacgc cggccgagct ctcggcgctc
     2881 cacgccgagc tcgtcgccga gatcgagcgc ctcgctctga cccatgatgg atggatccgc
     2941 ccctgggcaa agaagatcgc gcatcgcgcg ggcgagcgga cgctcctcgt cacgctgctg
     3001 ctcgacaatt cgggctcgct gcgcggccag cagatccggc cgatcgccgc ctgggcggcg
     3061 ctcgtgggcg acatcctgcg acactccggc gtcctggtcg aagtgctcgg tttcaccacc
     3121 gccgaatggc gcggcggccg ggcgcgccag cgctgggtga gcgacgggag ccccgaacgg
     3181 ccgggccggc tcaacgatct ccgctatatc gtctaccagg cgcatgacga cgacgcctat
     3241 tgcgccgcca atttcgccct gatgctcaag gacggcgtgc tcaaggagaa tatcgacggc
     3301 gaggccctgc tctgggcggc cgatcggcag cgcgggatcg ccgccgacga gaagatcatc
     3361 atcatcatgt cggacggcgc gccggtcgat gattcgaccg cggccgcgaa ccatcccgac
     3421 tatctccacg accacctcgt cgaggcgacg cgcctgatcg agggcgaccg gcagagcacg
     3481 ctctatggca tctatctcga gggcgactat cgtcccttcg cttattacac gcgctccgcc
     3541 accatccgcg cccccgagca gatggggctt gcggtgctgc gctttctggc gacggggtga
     3601 agcgatagcg cgacgctttg cgtcgcgtgc tatcgcttca ccccgtcgcc ggcgggcggc
     3661 agcggttgcg taagcaaccg cggagcccgc acagaggagg ctagcccgca cggtgcggct
     3721 ctcgcgattg caagagcaat cgctggcgcc gccgcggacg aaacgctgcg cgtttcgcct
     3781 agggaaattc caccatcgcc tcgcccgttg cgacgatctc gtcgcgctgg ttgcgcatct
     3841 cgatgtcgat cgtcacccag cgcgatcgtg acgtgacctg tttcgccttg aggcggccct
     3901 ggggccggag cgtgtcgccg gggcgggccg gcttcagcca gcgcgtctcg aggcggcgat
     3961 ggacggcgcc cttcgcatag gcccagtcgg tgagcatgcg ggtgatgagg gcgaaattcg
     4021 tcatgccatg catgatgacg ccgccgaaat tcgtcttgcc gaagctgccg gccatatacg
     4081 catcgtcgag atgcagcgga ttgtagtcga gcgaggcgtc gcagaagtcg cggatggtct
     4141 cgcgcgacac ggcaaaggcc gcgccgtcga tgacgtcgcc ggcggccaga gcgtcgaaga
     4201 tcccggtcat cggtgcgctc acatcggccg gatggtccag ccgcgccccg agcagatgac
     4261 ggcgccatgc tggttgaaga agacattgtc gtgcaccacg aagagtcgct ccttccggat
     4321 gaatttgtcg agcgcgcggg cctcgagggt gatcgtgtcg ccgggccgcg ccggcaggtt
     4381 gtagctccag gactggccgg cattcaccgt gccggggctg cgcatccagt cgtcggcggg
     4441 cgtgcaggcg aacatcagca ggatatggat ggcgggcggc gcgatgaggc cgccatggac
     4501 gctgcgccgc gcgtaatcct cgtcgaaata gagcgggtgg ag
//
LOCUS       DASYNS010000228         4208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_675079, whole genome shotgun sequence.
ACCESSION   DASYNS010000228 DASYNS010000000
VERSION     DASYNS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675079"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VGP50_07165"
     CDS             complement(<1..1310)
                     /locus_tag="VGP50_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8027193.1"
                     /translation="MSETELPPEGEYTAQSIKVLRGLDAVRKRPGMYIGDTDDGSGLH
                     HMVYEVVDNSIDEALAGHCDTISVTLNAEGSVTVTDNGRGIPVDIHDEEGVSAAEVIM
                     TQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSSRLDLRIWRGRREYFMRFIDSEPEAP
                     LAEVGAAPTLPGNGNGKAAHRTGTEITFLPSTKTFTKTEFDFQTLEHRLRELAFLNSG
                     VTLTLTDARGVEPKQVKMHYEGGLEAFVKYLDRSKQALHNTPIAIAAEREGTTVEVAM
                     EWTDSYHETMLCFTNNIPQRDGGTHLAGFRAALTRTLNNYASTGGVAKKEKVQLSGED
                     MREGLTCILSIKVPDPKFSSQTKDKLVSSEVRPVVDGIVADKLQQWLEEHPADAKRVV
                     AKVVEAAAAREAARKARDLTRRKGALDIANLPGKLADCEERDPA"
     gene            complement(1328..2530)
                     /gene="recF"
                     /locus_tag="VGP50_07170"
     CDS             complement(1328..2530)
                     /gene="recF"
                     /locus_tag="VGP50_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV8027194.1"
                     /translation="MKVMVLSAMASEPREAAAPVRSAVTRLLVTDFRGYASARLAADE
                     RPVVLTGPNGAGKTNLLEAISFLAPGRGLRRARLADIDRRVPPARPSATPPGAWTLAA
                     TLATTRGTVQVGTGRDAANEGGERRLLRIDGAPAKSQTTLAEHVSLVWLTPQMDRLFL
                     EGSAARRRFLDRLVYGFDPAHWARVGAYERAMRERARLLRDGPFDKSWLAALEERMAE
                     QGVAIVAARRHVTLRLDGVTSQARAPFPGARLTLVGGLQDWLDTMPALAAEEEFRRRL
                     SAARRADAESGTTTLGPHRGDFAVRHAESGMGAAECSTGEQKALLVAMVLAQARLLAE
                     ERGSAPVMLLDEVAAHLDDERRGALFGEIDAMGMQAWLTGTNPDLFEALWGRAQFFSV
                     ADAVIAPR"
     gene            complement(2593..3711)
                     /gene="dnaN"
                     /locus_tag="VGP50_07175"
     CDS             complement(2593..3711)
                     /gene="dnaN"
                     /locus_tag="VGP50_07175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV8027195.1"
                     /translation="MRLTIERAALLRALGHVQSVVERRNTIPILSNVLLRAEDGQVSL
                     SATDMDLEMVASVPAQGARKGATTAPAHTLYDIVRKLREGAQVELDASGDKGSMTLRS
                     GRSVFTLACLPPEDYPLMAGGDLAHGFSLSTTDLKRLIDRTRFAISTEETRYYLNGIY
                     LHAAKSGDVPMLRAVATDGHRLARMEIPLPEGAQGMPGVIVPRKTVTEIRKLIDELDQ
                     DVAISLSDTKIRFVFGNAVLTSKLIDGTFPDYDRVIPVNNDKILEVICKDFAEAVDRV
                     STISTEKSRAVKLAIERGNLTLSATSPENGTASEEIEVRYSAAPLEIGFNSRYLLDIT
                     EQIEGEGARFALADAASPTVVRDSADASALYVLMPMRV"
     gene            complement(4010..>4208)
                     /locus_tag="VGP50_07180"
     CDS             complement(4010..>4208)
                     /locus_tag="VGP50_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8027196.1"
                     /translation="VARPRQVAMYLCKQLTPRSLPEIGRKFGGRDHTTVMHAVRKIEE
                     LRGNDRTLAEDIELLRRMLET"
BASE COUNT          659 a         1406 c         1402 g          741 t
ORIGIN      
        1 tttgccggat cgcgctcctc gcaatcggca agcttccccg gcagattggc gatgtcgagc
       61 gcacccttgc ggcgcgtgag gtcacgcgct ttgcgcgccg cttcgcgagc ggccgcggcc
      121 tctaccacct tggcgacaac gcgcttggcg tccgcgggat gctcctcgag ccattgctgc
      181 agcttgtcgg cgacaatacc gtcgaccacc gggcggacct cggaagagac gagcttgtcc
      241 ttggtctggg aggagaattt gggatcgggc accttgatcg agagaatgca ggtgaggccc
      301 tcgcgcatat cctcgcccga gagctgcact ttctctttct tggcgacacc gccggtgctt
      361 gcgtagttgt tcagggtgcg cgtcagcgct gcgcggaagc ccgcgagatg cgtgccgccg
      421 tcgcgctgcg gaatgttgtt ggtgaagcac agcatcgttt cgtgatagct gtcggtccac
      481 tccatcgcca cctcgacggt ggtgccctcg cgctcggcgg cgatggcaat gggagtgttg
      541 tgcagcgcct gcttcgagcg gtcgaggtat ttgacgaagg cttcgagccc gccttcgtaa
      601 tgcatcttga cctgcttggg ctcgacgccg cgggcatccg tcaaggtcaa cgtcacgccc
      661 gaattgagga acgccagctc gcgcagccga tgctcgagcg tctggaaatc gaattcggtc
      721 ttggtgaagg tcttcgtgct cggcaggaag gtgatctccg tgccggtgcg gtgcgctgcc
      781 ttgccattgc cattgccggg cagcgtcgga gccgcgccga cctcggcgag cggcgcctcg
      841 ggctcgctgt cgatgaagcg catgaaatac tcgcgccggc cacgccagat gcgcaggtcg
      901 agccgtgagg acagcgcatt caccaccgac acgccgacgc catggaggcc gcccgagact
      961 ttgtaggaat tctggtcgaa cttaccgccg gcatggagct gagtcatgat gacctcggcg
     1021 gccgacacgc cttcttcgtc atggatgtcg acgggaattc cgcgaccgtt gtcggtgacg
     1081 gtgaccgagc cttcggcatt gagcgtcacg gaaatcgtgt cgcaatggcc ggcgagcgcc
     1141 tcgtcgatcg agttgtcgac cacctcatag accatgtggt gcaggcccga gccgtcatcg
     1201 gtgtcgccga tatacatgcc cggacgcttg cgcacggcgt ccaggcctcg cagcaccttg
     1261 atcgattgcg cggtgtactc gccctcgggc ggaagttcag tttcgctcat gtctcgtatc
     1321 ctatgggtca gcgcggcgcg atcacggcgt ctgccacgct gaagaattgc gcccggcccc
     1381 agagcgcctc gaacaaatcc gggttggttc cggtgagcca cgcctgcatg cccatggcgt
     1441 cgatctcgcc gaagagggcg ccgcgccgtt cgtcgtcgag atgcgcggcc acctcgtcca
     1501 agagcatcac tggtgcgctg ccgcgctctt ccgccaagag ccgcgcctgg gcaaggacca
     1561 tggcgaccag taatgccttc tgctcgcccg tcgagcattc tgctgcgccc atgccgctct
     1621 cggcgtggcg caccgcgaag tcgccgcgat gaggccccag cgtggtcgtg ccgctctcgg
     1681 catcggcgcg gcgcgcggca gagagccggc ggcggaactc ctcctcggca gcgagcgccg
     1741 gcatggtgtc gagccagtcc tgaagcccgc cgacgagggt cagccgcgcg ccaggaaagg
     1801 gcgcccgcgc ttggctggtg acgccgtcga ggcggagcgt gacgtggcgc cgcgccgcga
     1861 caatcgcaac gccctgttcg gccatgcgct cctcgagcgc tgcgagccac gacttgtcga
     1921 acggcccgtc gcgcagcaga cgcgcgcgtt cgcgcattgc ccgctcatag gcaccgaccc
     1981 tcgcccagtg tgcgggatcg aagccgtaga ccaagcggtc gaggaagcgg cggcgcgcgg
     2041 cgctgccttc gagaaagagg cggtccatct gcggcgtgag ccagaccagg ctcacatgct
     2101 cggccagtgt cgtctggctt ttggccggcg caccgtcgat gcgcaggagc cgccgctcgc
     2161 cgccctcgtt cgccgcatcg cgccccgtgc cgacctgcac cgtccctcgc gtggtggcca
     2221 gcgtcgccgc cagcgtccac gcacccggcg gcgtcgcgct cggccgcgct ggcggcacgc
     2281 gccggtcgat gtcggcgaga cgcgcgcggc gcaggccgcg acccggcgcc aggaaagaga
     2341 tcgcctcgag cagattggtc ttgcccgcgc cgttgggccc ggtcagcacg accggccgct
     2401 catcggccgc caagcgggcg gaggcatagc cgcggaaatc ggtcaccaag aggcgtgtca
     2461 cggccgagcg gaccggagcg gcagcttcgc gcggctcgct cgccatcgca ctcaacacca
     2521 tcactttcat atgggaattc ccgccctcca acgccagcct cggcgagggg ctcgccgccc
     2581 tattgtccac gcctaaacgc gcatcggcat gagcacgtag agcgcgctgg cgtcggcgct
     2641 gtcgcgcacc acggtgggcg acgcggcatc cgccagggcg aagcgtgcgc cttccccctc
     2701 gatctgctcg gtgatgtcga ggagatagcg ggaattgaag ccgatctcga gcggcgctgc
     2761 gctgtagcgc acctcgatct cctcgctcgc ggtgccgttt tccgggctcg tcgcggacag
     2821 cgtcagattg ccacgctcga tcgccagctt caccgcccgg ctcttctcgg tcgagatggt
     2881 cgaaacacgg tcgaccgctt cggcgaagtc cttgcagatg acttcgagga tcttgtcgtt
     2941 gttgaccggg atcacgcgat cgtagtccgg gaaggtgccg tcgatcagct tcgaggtcag
     3001 cacggcgttg ccgaagacga agcggatctt ggtgtcggag agcgagatcg ccacgtcctg
     3061 gtcgagttcg tcgatgagct ttctgatctc ggtcaccgtc ttccttggca cgatcacccc
     3121 cggcatcccc tgggcgcctt cgggcaacgg gatctccatg cgcgccaacc gatgaccgtc
     3181 ggtcgccacg gcgcgcagca tcggcacgtc cccacttttc gccgcatgga gatagatccc
     3241 attgaggtag tagcgggtct cctcggtgga gatggcgaag cgcgtgcggt cgatgagccg
     3301 cttcagatcc gtcgtcgaga gactgaagcc atgggcgagg tcgccgccag ccatcagcgg
     3361 atagtcttcg ggcggcagac aggcgagcgt gaagaccgag cgccccgagc gcagcgtcat
     3421 cgagcccttg tcgcctgaag cgtcgagttc cacctgcgcg ccttcgcgca gcttgcgaac
     3481 gatatcgtaa agcgtgtggg ccggcgcggt ggtggctccc ttgcgcgcac cctgcgccgg
     3541 caccgacgcc accatctcga gatccatgtc agtggccgac aacgagacct gtccatcctc
     3601 agcccgcagc agcacattgg acaggatcgg aattgtattg cgccgttcga cgacgctctg
     3661 cacatggcct aaggcgcgga gcagcgcggc gcgttcaatg gtcaatctca tggcggcgag
     3721 ttcacgctga tggtggcggt ttccagcacc cgaaaacccc ctcgggcacc ccgccgcaag
     3781 aggccggaaa cgaccctgcg gaacggtgta gcactatagc acgaggcggc gcggccgtat
     3841 acctccggag cggcggccgg gtggctgtcc gatgcgctct aagtcgttgg cccaagccgg
     3901 ttttcggaac gggcggatga cggacgaatc accgtctgct cggtgcggct cccgcgattg
     3961 ctgcgcaatc gctgacgccg ccaaacgcga gcggcctggc cgctcgcgtt taggtctcaa
     4021 gcatgcggcg cagcagttcg atgtcctcgg ccagcgtgcg gtcgttgccg cgcagctcct
     4081 cgatcttgcg cacggcatgc atgaccgtgg tgtggtcgcg cccgccgaat ttgcggccga
     4141 tctcgggcag cgagcgcggc gtcagttgct tgcagagata catcgccacc tgccgtggcc
     4201 gggcgacg
//
LOCUS       DASYNS010000229         3443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1143499, whole genome shotgun sequence.
ACCESSION   DASYNS010000229 DASYNS010000000
VERSION     DASYNS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143499"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VGP50_07185"
     CDS             <1..396
                     /locus_tag="VGP50_07185"
                     /inference="COORDINATES: protein motif:HMM:NF025908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027197.1"
                     /translation="MGTSLELLRFEGLVAAVDGEGMADNAVLQLTERGRAALDSLLQA
                     QLRAPVNDLNRLALLLKLRFLHHLPAAEQQAQLALMAESLDSERARLEELVRRHGTPG
                     LLAEWLAADIAMVSERITWLQRRSAAGSQ"
     gene            complement(561..1799)
                     /locus_tag="VGP50_07190"
     CDS             complement(561..1799)
                     /locus_tag="VGP50_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027198.1"
                     /translation="MRCSMGLSRTLPAHLLAIGIVALAAAAGATAGDKRFGPGVSDDE
                     ITIGQTMPYSGPSSAWGAEGLAELAYIKMINEQGGVNGRQIRLISLDDAYSPPRTVEQ
                     TRKLVEQEQVALIFSSLGTAPNTAIQKYLNGRKVPQLFIATGASKWSDPEHFPWTMGW
                     QASYLTEARIYAHYIREQRPDGKIAVLYQNDDYGKDYLQGLKDGLGDKYAAMVAAVAT
                     YEVSDPTVDSQIVTLQASGADIFVDITGPKFAAQAIRKVYDIGWKPLHLLNTVGSAVG
                     AVLQPAGLEKSVGIITARYYKDPSDPQWQKDPAFAEWLAWMQRYNPEGNLSDYYNVLG
                     YSTAMTMVHVLKQCGDDLSRENILRQATNIQHLELPMLLPGVTINTSPTDYRPIKQMR
                     LVRFDGKSWTLFGELQGDGM"
     gene            complement(1891..2421)
                     /locus_tag="VGP50_07195"
     CDS             complement(1891..2421)
                     /locus_tag="VGP50_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HEV8027199.1"
                     /translation="MASVARVSVLCRDRVGLVSAIADCLFQAGVNLRDTTFAVLGEGA
                     EFTSVCELPRGFGADDLRAELALLPELAGAQLDVHPYGFDPRPGPMGRITHRVEVSGG
                     DQLGLIARLSEIFTQNGANIVRLDAQKLPEAEGGRYVTRFAVWLPSERAALCLAAVDG
                     TAGSLGLTTRVEAVRP"
     gene            complement(2438..3178)
                     /locus_tag="VGP50_07200"
     CDS             complement(2438..3178)
                     /locus_tag="VGP50_07200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8027200.1"
                     /translation="MSGEVRTLLLTGASRGIGHATVRRFSDAGWRIITASREAVPAEC
                     KRDPNWMHHITADLGDVGDVERFIAEAQTTIGAGALHALVNNAGVSPKTPFKERLGCL
                     NGDLAAWHEVFQLNFFAPLMLSRGLAAPLARGKGAIVNVTSIAGHAVHPFAGSAYSTS
                     KAALSALTREMAVEFAQLGVRVNAVAPGEIETAMLSPETEVLIPRIPLHRLGTPDDVA
                     HTIFYLCSDASSYVTGTEIFVTGGQHLY"
BASE COUNT          572 a         1152 c         1149 g          570 t
ORIGIN      
        1 atgggcacgt cgctcgagct cttgcgcttc gaggggctcg tcgccgcggt cgatggcgaa
       61 ggcatggccg acaatgcggt gctccagctc accgagcgcg gccgcgccgc cctcgacagc
      121 ttgctgcagg cgcagctgcg cgctcccgtc aacgatctga accgcctcgc cctgctcctc
      181 aagctgcgct tcctccacca tctgccggcc gccgagcagc aggcacagct cgccctcatg
      241 gcggaatcgc tcgacagcga aagagcgcgc ctcgaagagc tcgtccgccg ccacggcacg
      301 cccggcctcc tcgccgagtg gctcgccgcc gacatcgcca tggtgagcga gcgcatcacc
      361 tggctccagc gccggtcggc cgccgggagt caataacccc tctccccttg cgggagaggg
      421 aggggcccac tgcgaagcct cagcgaagca gtgggagggt gagggaccct ctcggaatcc
      481 aatcctgcac ggtgcggctc tcgcgacgag cgcttcgcgc tgacgccgcc gccgacgaac
      541 gcacttcgcg cgttcgcggc ttacatcccg tcgccctgaa gctcgccgaa gagcgtccag
      601 ctcttgccgt cgaagcgcac gagccgcatc tgcttgatcg gacgatagtc cgtcgggctg
      661 gtgttgatcg tgacgccggg cagcagcatc ggcagctcca gatgctggat attcgtcgcc
      721 tggcgcagaa tgttctcgcg gctcagatca tcgccgcatt gcttcaagac atgcaccatg
      781 gtcatcgccg tgctgtagcc cagcacgttg taatagtcgg agagattgcc ctcgggattg
      841 tagcgctgca tccaggcgag ccattccgcg aaggcgggat ccttctgcca ctgcgggtcg
      901 cttgggtcct tgtagtatcg cgcggtgatg atgccgaccg atttctccag gcccgccggc
      961 tggagcacgg caccgacggc gctgcccacg gtattgagaa gatgcagcgg cttccagccg
     1021 atgtcgtaga ccttgcggat cgcctgggca gcgaatttgg ggccggtgat gtcgacgaag
     1081 atatcggcgc cggaggcttg cagcgtgaca atctgtgaat cgacggtggg atcgctcacc
     1141 tcataggtcg cgaccgccgc caccatcgcg gcatatttgt cgccgaggcc gtccttcagc
     1201 ccctggaggt agtccttgcc gtaatcgtcg ttctgataga ggacggcgat cttcccgtcg
     1261 ggccgctgct cacggatgta atgcgcatag atgcgcgcct ccgtcagata gctggcttgc
     1321 caacccatgg tccaggggaa gtgctcggga tccgaccatt tgctggcgcc ggtcgcgatg
     1381 aagagctgcg gcactttgcg cccgttcagg tacttctgga tcgccgtatt cggcgctgtg
     1441 ccgagcgagc tgaagatgag tgcgacctgc tcctgctcga cgagcttgcg cgtctgctcg
     1501 acggtcctgg gcgggctata ggcgtcgtcg aggctgatca ggcggatctg gcggccgttc
     1561 acgccgcctt gctcgttgat catctttatg tacgccagtt cggccaggcc ttcggcgccc
     1621 caggcggagc tcgggccgct atagggcatc gtctggccga tggtgatttc gtcgtcgctg
     1681 acgcccggcc caaagcgctt atcgccggcg gtcgcgccgg ccgctgcggc cagggccaca
     1741 atgccgatcg ccaggagatg cgcgggcaag gtacgggaga ggcccatcga gcagcgcatg
     1801 cttcacctcg ctgtccggcc tcggccgtgc cgaggcgacg ctcgcctctc tattctacaa
     1861 gcggttgggg cggcgacaaa gcgcggccgc tcacgggcga accgcctcga cgcgggtggt
     1921 caatccgagg ctgccggccg tgccgtcgac cgcagcgagg cagagggccg cgcgctcgga
     1981 tggcagccac accgcaaacc gcgtcacata gcgcccgcct tcggcctccg gcagcttctg
     2041 tgcgtcgagc cgaacgatgt tggcgccgtt ctgggtgaag atctccgaga gccgcgcgat
     2101 gagaccgagc tggtcgccgc cgctcacctc gacgcgatgg gtgatccgcc ccatcggacc
     2161 cggccgcggg tcgaagccat agggatggac atcgagctgc gcgcccgcca gctccggcag
     2221 cagcgcgagc tcggcacgca aatcatcggc cccgaagccg cgcggcaact cgcaaaccga
     2281 cgtgaactcg gcaccctcgc ccagcaccgc aaacgtggta tcgcgcagat tgacgccggc
     2341 ctggaacagg cagtcggcga tcgccgaaac caggccgacc cgatcgcgac acagcacgga
     2401 gacccgggcc accgatgcca tctctacctc ccctcgctta atagagatgc tgtccgccgg
     2461 tcacgaagat ctccgtgccc gtgacatagc tgctcgcgtc gctgcagaga tagaagatcg
     2521 tatgggcgac gtcgtcgggc gtgcccaggc gatggagcgg gatgcgcggg atcagcactt
     2581 cggtctcggg cgacagcatc gcggtctcga tctcgcccgg cgccaccgca ttgacgcgca
     2641 cgccgagctg cgcgaattcg acggccattt cgcgggtcag cgccgagagc gccgccttcg
     2701 aggtcgagta ggcggagccg gcaaaaggat ggacggcgtg gccggcgatc gaggtcacgt
     2761 tgacgatggc gcccttgccg cgcgccagcg gcgccgccag gccccgcgac agcatcagcg
     2821 gcgcaaagaa attgagctgg aagacttcat gccaggcggc gagatcgccg ttgagacagc
     2881 cgaggcgctc cttgaacggc gtcttcggcg agacgccggc attgttgacg agggcatgga
     2941 gcgcgcccgc gccgatcgtc gtctgcgctt cggcgatgaa gcgctcgacg tcgccgacgt
     3001 cgccgagatc cgcggtgatg tgatgcatcc agttgggatc gcgcttgcac tcggcaggca
     3061 cggcctcgcg cgatgcggtg atgatgcgcc agccggcatc gctgaaccgg cgcaccgtgg
     3121 catggccgat gccgcggctc gcgccggtaa ggagaagcgt cctgacctcg ccgctcatgg
     3181 cgcgagctta ggcgagcggc cccgccaagg aaagcctgcc agaagcgatg aacgctgctg
     3241 agcccaggcg cgcgctatag ggcctgctac gcaccaagaa cgacgcggcg caggagaaag
     3301 atgaacggta gagtcgagga gaggcgcggt gtcgcgcgag cgctccgttt cctctccccg
     3361 ctcatcgaac cggacgtgcg gatttcccgc atccggctct ccgactggct tcgtggcagg
     3421 ctcacgacac ggcgccggga ttg
//
LOCUS       DASYNS010000230         7745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4421004, whole genome shotgun sequence.
ACCESSION   DASYNS010000230 DASYNS010000000
VERSION     DASYNS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4421004"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VGP50_07205"
     CDS             <1..223
                     /locus_tag="VGP50_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027201.1"
                     /translation="GRAADLALFKLDELRFAGHGDAIAALVRGGAHAADRVMVNGRWV
                     VEDGRLPGLDLPALVARQQHFARGLRAAV"
     gene            306..452
                     /locus_tag="VGP50_07210"
     CDS             306..452
                     /locus_tag="VGP50_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027202.1"
                     /translation="MSGMLSTLRLCAFVASLAATAAALSACSSQNGDNTGTEYINALG
                     GYHR"
     gene            complement(462..875)
                     /locus_tag="VGP50_07215"
     CDS             complement(462..875)
                     /locus_tag="VGP50_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEV8027203.1"
                     /translation="MDEIRIIAGPFAFGARFETAAAPDTCVRFRSLLPYRQRLIHVRW
                     SGEACWIPLGDTKFDLGCENHTSYPAPGQIILYPGGISETEILLAYGSVRFASKVGQL
                     AGNHFLTITDGAAQLRALGEHVLWQGAQEIRFEAP"
     gene            complement(1128..2042)
                     /locus_tag="VGP50_07220"
     CDS             complement(1128..2042)
                     /locus_tag="VGP50_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV8027204.1"
                     /translation="MSVSQLSQPRALRYVEAVARCGSIRRAAESLSIASSAVNRMILD
                     LERALDAKLFERLPRGVRLTSAGEYLLLHIRRSSADFETVRARIDGLKGVQRGHVAIA
                     AIEAAIEPFLARAIAAFHLSHRLVSYDVRIAGALDIPEAVASGQVDLGLTLNAPRSAR
                     FVTLATVPYFLHAFVSAGHPLARHRRLRLSDCVGYPLAMGDATLGGRQRLEQAFAQTA
                     LDFRPFLSSNSMALMMAAVRSSDAICFQALPVARHHVAGGLAAIPLADPAVGEVELAL
                     LADKRRALSTAVATFAVEIARAFRESAT"
     gene            2195..3310
                     /locus_tag="VGP50_07225"
     CDS             2195..3310
                     /locus_tag="VGP50_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031302833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-opening amidohydrolase"
                     /protein_id="HEV8027205.1"
                     /translation="MATSRRALVFCVKMAHPGDTSALAALIAGGDVAATEIVAIFGKT
                     EGNGCVNDFTRPYAIESLKTALAPLLGCGRDELAERIAMVMSGGTEGGLSPHFLVFAV
                     RAMEAPAGSGKRLAIGRAATRAFLPEELGRLPQIRETAAAVGRAIAAAAISSPDDVHF
                     VQIKCPLLTAERHAEATSRGALPATHDTYASMGLSRGASALGVGLALGELDEAALADD
                     VVCRDLGLWSARASASAGIELMNSEVVVLGNSAAWSGDLVIAHAVMRDAIDVLAAHEA
                     LAGIGLAAAQQLTGAQRERLRAVLAKADPASSGRIRGARHVMLDDSDILATRHARAFV
                     GGALAGLMGRTDLFVSGGSEHQGPDGGGPIAVIGVAP"
     gene            3417..4421
                     /locus_tag="VGP50_07230"
     CDS             3417..4421
                     /locus_tag="VGP50_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027206.1"
                     /translation="MSARYAVELVAVTKRYGATVAVDNVSLRIPEGNYCCLLGPSGCG
                     KTSMLRMIAGHELISDGDILISGQNVGSLPPVSRGTAMMFQSYALFPHLSSLDNVAFS
                     LKMRGVAKAERRAKAREFLALIHMSDYADRLPAQLSGGQQQRVALARALVTHPKVLLL
                     DEPLSALDPFLRGRMREELKRLQNELGITFIHVTHSQEEAMALADLVVVMESGRIQQA
                     DAPRAIFDRPSTAFVAQFLGGHNLIPRAGDGALAVRTDRCRVLDAATAAEPSLLGQVS
                     AIEYQGSRVQVTLAVDGGIRAVSHLPDEVFYAHPFRIGDRARLAWSDADAHPVSVAIN
                     "
     gene            4432..5739
                     /locus_tag="VGP50_07235"
     CDS             4432..5739
                     /locus_tag="VGP50_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PotD/PotF family extracellular solute-binding
                     protein"
                     /protein_id="HEV8027207.1"
                     /translation="MSKRRPPARGKHGKISRRRFLEGSAAAAAGVAGATAITGFPMVW
                     AQNLKDITLRQFGTGTSNQNAIAERCKADLGITLEMTASDTDAAVQRAVTQPRSYEIA
                     DIEYFSCKKVFPTGVLQPLEVKRIKYFDKIVPIFITGKLTPESEIAQGTAPHTVEFVG
                     GKDSHKFAKQPTEWATMIPTIYNADTLGIRPDLISRPIKNWRDLVDPAFKGKASILNI
                     PGIGMMDAAMVMESLGEIKYKDKGNMTRDEIDRTIAFLIATKKSGQFRAFWKSFDESV
                     NLMASGEVIIQSMWSPAVAAVRARGIPCVYQPLAEGYRAWAGGLAIASHVKGPMLDAA
                     YEYINWYLSGWVGAYLNRQGYYTAVLETAKQYMSADEWSYWMDAKPAQGDIVSPEGKV
                     MEKAGAVRDGGAFAERMGRVVCWNSVMDEDRYMIEKWNEFIAA"
     gene            5736..6677
                     /locus_tag="VGP50_07240"
     CDS             5736..6677
                     /locus_tag="VGP50_07240"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8027208.1"
                     /translation="MSAFEIAGTAPAATRPPARSRRDRRAYFQAAPLAAIFACFLVFP
                     IIAILVVSVFDYDSVRIIPAFTLENYRELLFSRVTWQTYLNTFKFAAATWAVTLAIGF
                     TLAYFLAFYVRSTLWQIALSLICTVPFWTSNIIRMISWIPFLGRNGLLNMALERLGVI
                     HQPLEFLLFSDFAVVLALVHLNTIFMVIPIFNSMMRIDRSLIEAARDAGASAVQILWN
                     VILPLTKTGIAIGSIFVLSLVMGDFITVRLLSGGQSASVGLMMANSIGVLQYPAAAAN
                     AVLLLLLVLGMVAGILRTVDIRRELSVPAAQGRRGDG"
     gene            6670..7494
                     /locus_tag="VGP50_07245"
     CDS             6670..7494
                     /locus_tag="VGP50_07245"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8027209.1"
                     /translation="MGERRSLGFYLLATFFGLYVLFLYGPTITIAVLSFQGPNGGLTF
                     PLNGVSFHWFGNLFERQLVGDFAGSLKRSLCLALLVTLATVTISVAAGLAYRKRFAGD
                     TVLFYLAIASLVVPSILVSLGIGLLFDLLGIDPDWYSSAFGAQLTWTLPFGLLIMFAV
                     FSRFNPSYEEAARDLGATPWQTLRHVVLPIVAPSILGVALFGFTLSYDEFARTLLTAG
                     AENTLPLEIYGMTTNITTPVLFALGTLTTAFSFIVIGTCLIGVVALQRRRSRRGGG"
     gene            7503..>7745
                     /locus_tag="VGP50_07250"
     CDS             7503..>7745
                     /locus_tag="VGP50_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEV8027210.1"
                     /translation="MRILVVNPNTTASMTEKIASAARQVASDATLIEAVTSKMGPESI
                     EGYYDEAFAVPGVVQAIMAGEADGVDGAVIACFDDTG"
BASE COUNT         1232 a         2631 c         2513 g         1369 t
ORIGIN      
        1 cgggcgcgcc gccgatctgg cgctcttcaa gctcgacgag ctgcgctttg ccggtcatgg
       61 cgacgcgatc gccgcactcg tccgcggcgg cgctcatgcg gccgaccgcg tgatggtcaa
      121 tggacgatgg gtggtggagg atggccgctt gcccggactc gatttgccgg cgttggttgc
      181 gcgccagcag catttcgccc gcggcctccg ggccgcggtc taggagtggc gcgcgatgcg
      241 cgcgtcatgt tatcgacgga gcgtgccgtg tcgcggcgcg ctgcgcaaag aaggagagac
      301 gagtcatgtc agggatgttg agcaccttgc gcctgtgcgc tttcgtcgcg agcctcgcag
      361 cgacggcggc ggcactctcc gcctgctctt cgcagaacgg ggacaacacg ggtaccgaat
      421 atatcaacgc cctgggcggg tatcaccgct gacgtctggc gttacggcgc ctcgaagcgg
      481 atctcctgcg cgccttgcca gaggacgtgc tcgcccagcg cgcggagctg agcggcgcca
      541 tcggtgatcg tcaggaagtg gttgccggcg agctgcccga ctttgctcgc gaagcggacg
      601 ctgccatagg ccagcaggat ctcggtctcg ctgatgccgc ccggatagag aatgatctgc
      661 ccgggcgccg ggtagctggt gtggttctcg caacccaggt cgaatttcgt gtcgcccagc
      721 gggatccagc atgcctcgcc gctccagcgc acatgaatga ggcgctggcg atagggcagc
      781 aggctccgga aacgcacgca tgtgtcgggg gccgccgccg tctcgaagcg cgcgccgaaa
      841 gcgaaggggc cggcgatgat gcggatctcg tccatgctcg acattctccc gttgctcttc
      901 ccatcccctc tcccgcatcg cgggagaggg tggcgcgggc gcagcctgcg ccgggtgagg
      961 ggaggcatca aacaaaggca gctttgcctg agacgtcccc tcaccctccc agcgctacgc
     1021 gctgggtccc tccctctccc gcgatgcggg agaggggatg tttggcttga ttagacgcga
     1081 atgtccacgc gatccggctc gaacattggc atcaatagaa attcaaatca ggtcgcgctt
     1141 tcgcggaatg cccgcgcaat ctccacggcg aaggtcgcca cggccgtcga gagcgcgcgc
     1201 cgcttgtcgg ccagcagcgc cagctcgacc tcgcccaccg ccggatccgc aagggggatc
     1261 gccgccaatc cgccggcgac gtggtggcgg gcgaccggca gcgcctggaa gcagatggcg
     1321 tcggaagatc tgaccgccgc catcatcaac gccatcgagt tcgacgagag aaacgggcgg
     1381 aaatcgagcg ccgtctgcgc gaaagcctgc tccagccgct gccgtccgcc cagcgtcgca
     1441 tcgcccatgg cgagcggata gccgacgcaa tcgctcaagc gcagccggcg gtggcgcgcc
     1501 agcgggtgcc cggcgctgac gaaggcgtgg aggaagtagg ggacggtcgc gagcgtgacg
     1561 aatcgcgccg accgcggcgc attgagcgtg aggccgagat cgacttgccc tgacgccacg
     1621 gcctccggga tatcgagcgc tccggcgatg cgcacatcgt aggagacgag ccggtggctg
     1681 aggtggaacg cggcgatggc ccgcgcgagg aacggctcga tcgccgcctc gatggcggcg
     1741 atcgcgacat ggccgcgttg cacgcctttg aggccatcga tccgggcgcg gaccgtctcg
     1801 aaatcggcgc tgctgcggcg gatatgcagc agcagatatt cgcccgccga ggtcaggcgg
     1861 acgccgcgcg gcaagcgctc gaacaatttg gcgtcgagcg cgcgttcgag gtcgaggatc
     1921 atgcgattga ccgccgaaga ggcgatgctc agcgattccg ccgcccggcg gatcgacccg
     1981 cagcgcgcca ccgcttcgac atagcggagc gcccgcggct gggacagctg agagacgctc
     2041 atgcccatac ggtcctatct cctaccgccg catgatgcga aaatcgggcc aagcgagcga
     2101 aaaaagagag cgctcggggc gattattgcc gctaccgcgc ggcgcaagcc ggcttttagc
     2161 gtagggcatt cggcattgcg cgaaagctgc ggccatggcg acatcgcgac gagctctggt
     2221 cttctgtgtg aagatggcgc atccgggcga cacgagcgcc ctcgccgcgc tcatcgccgg
     2281 cggagatgtc gccgccactg agattgtcgc gatcttcggc aagaccgaag gcaatggctg
     2341 cgtcaacgac ttcacgcggc cctacgccat cgaatccttg aagacggccc tcgcgccgct
     2401 cctcggctgt ggccgggacg aactggcgga gcgcatcgcc atggtgatgt ccggcggcac
     2461 ggaaggcggg ctctcgccgc atttcctggt cttcgccgtg cgcgcgatgg aagcgccggc
     2521 cggctccggc aagcgcctcg ccatcggccg cgcggcgacg cgcgctttcc tgccggaaga
     2581 gctcgggcgt ctgccgcaga tccgcgagac ggcggccgcg gtcgggcgcg cgatcgctgc
     2641 ggcggccatc tcgtcgcccg acgatgttca cttcgtacag ataaagtgtc cgctgctcac
     2701 ggccgagcgc catgccgaag cgacttcacg cggcgcgctg ccggcgaccc acgacaccta
     2761 tgcgtcgatg gggctgtcgc gcggcgcttc cgctctgggg gtcggtctcg ctctcggcga
     2821 gctcgacgag gcggcgctgg ccgatgacgt ggtgtgtcgc gatctcggcc tgtggtcggc
     2881 gcgggcgagc gcgtccgccg gcatcgagct catgaacagc gaggtcgtcg tgctgggcaa
     2941 cagcgcggcc tggtccggcg acctcgtcat cgctcacgcg gtgatgcgcg atgccatcga
     3001 tgtgctcgcc gcccatgagg cgctcgccgg catcggtctc gcggcagcgc agcagctcac
     3061 cggtgcgcag cgcgagcggc tgcgcgccgt cctggccaag gccgatccgg catcctccgg
     3121 ccgcatccgc ggcgcgcgcc atgtcatgct cgacgacagc gacattctgg cgacgcggca
     3181 tgcgcgcgcc ttcgtcggcg gtgcgctggc cggactgatg ggacgaaccg acctcttcgt
     3241 ctcgggcggc agcgagcatc agggaccgga tggcggcggc cccattgccg tgatcggcgt
     3301 cgcaccgtga acgggctgcg cggggcttcc ggcctcgcgg gccatggcac gctccttgct
     3361 acgctttttc cggcgctgtc ggcgcgccct ctgtgcggcc gaggacgcag agaaaaatga
     3421 gcgcgcgcta tgccgtcgaa ctggtcgcgg tcaccaagcg ctacggcgcg accgtagcgg
     3481 tcgacaatgt ctcgttgcgc attcccgagg ggaactattg ttgcctcctc gggcccagcg
     3541 gctgtggcaa gaccagcatg ctgcggatga tcgccgggca tgagctgatc tccgacggtg
     3601 acatcctcat ctctggtcaa aatgtgggca gtttgcctcc ggtttcgcgg gggacagcga
     3661 tgatgttcca gagctatgcg ctgtttcccc atctgagcag cctcgacaat gtcgccttca
     3721 gcctcaagat gcggggcgtc gccaaggcgg agcggcgggc gaaagcgcgc gaattcctgg
     3781 cgctcatcca catgagcgac tacgccgacc ggttgccggc gcagctctcg ggcggtcagc
     3841 agcagcgcgt ggcgctggcg cgtgcgctcg tcacccatcc caaggtgctg ctcctcgacg
     3901 agccgttgtc ggcgctcgat cccttcctgc gcggccgcat gcgcgaggag ctgaagcggc
     3961 tgcagaacga gctcggcatc accttcattc atgtcacaca ttcccaggag gaggcgatgg
     4021 cgctcgcaga cctcgtggtg gtgatggaaa gcgggcgcat ccagcaggcc gacgcgccgc
     4081 gcgccatctt cgaccgcccg agcaccgcct tcgtcgcgca gtttctcggc ggccacaacc
     4141 tcatcccgcg ggccggcgac ggcgccttgg ccgtgcgcac cgatcgctgc cgcgtcctcg
     4201 acgccgcgac ggccgccgag ccgagcctcc tcggccaagt gagcgcgatc gaatatcagg
     4261 gcagccgagt ccaggtgact cttgccgtcg atggcggcat ccgggcggtc tcgcatctgc
     4321 ctgacgaggt cttctacgcc catcccttcc gcatcggcga ccgcgcgcgt ctcgcctggt
     4381 cggacgccga tgcgcatccc gtctctgtgg ccatcaacta ggggagcagt catgagcaag
     4441 cggcgtccgc ccgcgcgggg caagcacggc aaaatcagtc ggcggcgttt tctcgagggg
     4501 agtgccgcag ccgcggccgg cgtcgccggc gccactgcga tcaccggctt cccgatggtc
     4561 tgggcgcaga atctcaagga cattacgctg cgccagttcg gcacgggcac ctcgaatcag
     4621 aacgccattg ccgagcgctg caaggccgat ctcggcatca ccctcgaaat gacggcgagc
     4681 gacaccgacg ccgccgtcca gcgcgccgtc acccagccgc gctcctacga gatcgccgac
     4741 atcgagtatt tcagctgcaa gaaagtcttc ccgacgggcg tgctgcagcc gctcgaggtc
     4801 aagcgcatca aatatttcga caagatcgtg ccgatcttca tcacgggcaa gctgacgccg
     4861 gaatcggaaa tcgcgcaagg cacggcgccc cataccgtcg aattcgtcgg cggcaaggac
     4921 tcgcacaaat tcgccaagca gccgaccgaa tgggcgacga tgattccgac catctacaat
     4981 gccgatacgc tcggcattcg ccccgacctg atcagccgcc cgatcaagaa ctggcgcgat
     5041 ctcgtcgatc ccgccttcaa gggcaaggcc tcgatcctca acatccccgg catcggcatg
     5101 atggacgcgg cgatggtcat ggagtcgctg ggcgagatca aatacaagga caagggcaac
     5161 atgacccggg acgagatcga ccgcacgatc gcgttcctca tcgccacgaa gaagtccggg
     5221 cagttccgcg ccttctggaa gagcttcgac gagagcgtca acctgatggc ctcgggcgaa
     5281 gtcatcatcc agtcgatgtg gtcgccggcg gtggcggcgg ttcgcgcccg cggcattccc
     5341 tgcgtctatc agccgctcgc cgaaggctat cgcgcctggg ccggcgggct cgccatcgcg
     5401 tcccatgtca aagggccgat gctcgatgcc gcctacgagt acatcaactg gtacctgtcc
     5461 ggctgggtcg gcgcctatct caatcgtcag ggctactaca ccgcagtgct cgagaccgca
     5521 aaacaataca tgtcggccga cgaatggagc tactggatgg atgccaagcc ggcgcagggc
     5581 gatatcgtca gccccgaagg caaggtcatg gaaaaggccg gagcggtgcg cgatggcggc
     5641 gccttcgccg agcgcatggg gcgcgtcgtc tgctggaact cggtcatgga cgaggaccgc
     5701 tatatgatcg agaagtggaa cgagttcatc gccgcgtgag tgcgttcgag atcgcgggca
     5761 cggcgcctgc agccacccgc ccgccggccc gatcgcgccg cgaccgtcgc gcttattttc
     5821 aggccgcgcc gctcgcggcg atcttcgcct gcttcctggt cttcccgatc atcgccatcc
     5881 tggtggtgag cgtcttcgac tatgattcgg tccggatcat ccccgccttc acgctcgaga
     5941 attatcgcga gctcttgttc tcgcgggtga cgtggcagac ctatctcaac accttcaaat
     6001 ttgccgctgc gacctgggcc gtgacgctcg cgatcggctt cacgctggcc tatttcctgg
     6061 cgttctatgt gcgctcgacg ctctggcaga tcgcactttc gctgatctgc acggtgccgt
     6121 tctggacgtc gaacatcatc cgcatgatct cgtggatccc gtttctcggg cgcaacggcc
     6181 tcttgaacat ggcgctcgag cgcctcggcg tcattcacca gccgcttgag ttcctactct
     6241 tctcggattt cgcggtcgtg ctcgccctcg tccatctcaa caccatcttc atggtcatcc
     6301 cgatcttcaa ttcgatgatg cgcatcgacc gctccctcat cgaggcggcg cgcgacgccg
     6361 gagcgagcgc agtgcagatc ctgtggaacg tcatcctgcc gcttaccaag accgggattg
     6421 cgatcggctc gatcttcgtt ctgagcctcg tcatgggcga tttcatcacc gtgcgccttt
     6481 tgagcggcgg gcagagcgcc tcggtcggcc tgatgatggc gaattcgatc ggggttctgc
     6541 agtatccggc ggccgccgcc aatgcggtgc tgctcctgtt gctcgtcctg ggcatggtcg
     6601 ccggcatcct gcgcaccgtc gacatccggc gcgagctctc cgtgccggcg gcacaaggga
     6661 ggcgcggcga tgggtgaacg ccggtccctc ggcttctatc tcctcgccac cttcttcggg
     6721 ctttacgtcc tcttcctcta cggcccgacc atcaccatcg ccgtgttgtc cttccagggg
     6781 ccgaatggcg gcctgacctt tccgctgaat ggcgtctcgt tccactggtt cggcaatctg
     6841 ttcgagcggc agctcgtcgg cgattttgcc ggctcgctca agcgctcgct ctgcctcgcg
     6901 cttctcgtga cgctggcgac ggtgacgatc tccgtcgccg ccggcctcgc ctatcgcaaa
     6961 cgcttcgccg gcgataccgt gctgttctat ctcgccatcg ccagcctcgt cgtgccctcg
     7021 atcctggtca gcctcggcat cggtctgctg ttcgacctcc tcggcatcga ccccgactgg
     7081 tacagctcgg ccttcggcgc acagctcacc tggacgctgc ccttcggcct tctgatcatg
     7141 tttgccgtgt tcagccgctt caatccgtcc tatgaggagg cggcgcgcga tctcggcgcc
     7201 acgccctggc agactctgcg ccatgtcgtc ctgcccatcg tcgcgcccag catcctcggc
     7261 gttgccctgt tcggcttcac gctctcctat gacgaattcg cgcgcacgct gctgaccgcc
     7321 ggcgccgaga acaccttgcc gctcgagatc tatggcatga cgacgaacat tacgacaccc
     7381 gtgctcttcg cgctgggcac gctcaccacc gccttctcct tcatcgtcat cggcacctgc
     7441 ctcatcggtg tcgtcgcgct gcagcggcgc cgctcgcggc gcggcggcgg ctgatcccgc
     7501 acatgcgcat cctcgtcgtc aaccccaaca cgacggcgtc gatgaccgag aagatcgcgt
     7561 ccgcggcccg ccaggtcgcg tccgacgcca cgctcatcga agcggtcact tcgaagatgg
     7621 ggccggagag catcgaaggc tattacgacg aggccttcgc cgtgccgggc gtcgtgcagg
     7681 cgatcatggc cggcgaagcc gatggcgtcg atggcgccgt cattgcctgc ttcgacgata
     7741 ccggc
//
LOCUS       DASYNS010000231         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1144750, whole genome shotgun sequence.
ACCESSION   DASYNS010000231 DASYNS010000000
VERSION     DASYNS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144750"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..678
                     /locus_tag="VGP50_07255"
     CDS             364..678
                     /locus_tag="VGP50_07255"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV8027211.1"
                     /translation="MKKFKVTFRPLAENDLFRLYEYISEQSGHERAAGYIDRIEATCR
                     DLETFPNRGTRRNDIRPDLRTMGFERRVTIVFEVKKTEVVIVRILYGGQDDRVIGDTT
                     QE"
     gene            complement(799..1443)
                     /locus_tag="VGP50_07260"
     CDS             complement(799..1443)
                     /locus_tag="VGP50_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV8027212.1"
                     /translation="MSLPVIVIGGGGHGRVVIEALRRAGVAVTGVIDPAQGVTAALPE
                     GVPWLGGDEALASYPPDGYRLVNGVGSIGDVSRRRVFEKMKAAGYSFAQVAHPAATIA
                     LAGISLGEGCQIMAGAVVQPGTRIGANAIINTHAAVDHDCIIGDHAHIAPGAVLCGDV
                     VVGEETHVGASAVVIQGRRIGAACLIGAGAIVLRDVPDRMIVYSHSDWRARLRG"
     gene            complement(1440..>2398)
                     /locus_tag="VGP50_07265"
     CDS             complement(1440..>2398)
                     /locus_tag="VGP50_07265"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEV8027213.1"
                     /translation="TFRADRIATRAAAKAEYQKSDGAAESQHAMLERLELDEAAHAAL
                     VPYCAERGIEFLSTPFDLPSVDLLVRRHALARVKISSGDLTNGPLLLHAARSGAALIL
                     STGMADLGEVEAALGVVAFGLTDPAALPGEAAFEAAYASAAGQQALHARVTLLHCTSQ
                     YPAPAATVNLRAMDTLHGAFGLAVGYSDHTLGTTVAVAAAARGAVMIEKHFTLDRSLP
                     GPDHQASLEPDELRRMVAAIRETEAALGSPRKAPVAGEIAMRAVARKCLVAARAIGRG
                     EAFTAENLTTKRAPGGVAAMRYWEALERTADRDYAADEPILL"
BASE COUNT          404 a          855 c          768 g          371 t
ORIGIN      
        1 gcatcgcggc caaaggactt gaacgagcac gccagcccaa ctagtatgac actgtcatag
       61 tagggagcat cgtttcccat aaggcaggag acctatgccc cgccgcagtc gaccgatcac
      121 cgtgaccctt ggcgaccttc aaacccacgt cgaggcacgt gtgaagtccg gagcttacgc
      181 gtccgcgagc gaagtgctgc gcgcggccgt gcgggccctc gatcgcgagg aggcggctgt
      241 caacgactgg ctacgccagc gcgttgatga agctctcgcg gatcctcggc cgaatgtccc
      301 ggcgcgcgat gtcttcaagc gcctccgtga gcatcatgct aagagtgtga gagccgctcg
      361 gcggtgaaga agttcaaggt tacctttcgg ccgctcgccg aaaatgacct cttcagactc
      421 tatgaataca tctcggaaca gtccggtcat gagcgcgcgg ccgggtatat cgatcggata
      481 gaggcaacct gccgcgacct cgagacattt cccaaccgcg gcacccgtcg caacgatatc
      541 cggcctgacc ttcggacgat ggggttcgag cggcgtgtca caattgtatt tgaggtcaaa
      601 aagaccgaag tcgtgattgt tcggatctta tacggaggcc aagatgaccg agtcattggg
      661 gataccactc aggaatgaaa cttaaaggcc agaacagctg cggatattat cgttgccggg
      721 actttgcgat gaggatcgcc gcatcgcgcc cctccctctc ccacaagggg agaggggaag
      781 gttacgccac atcgagactc acccccgcag ccgcgcgcgc cagtcgctgt gcgagtagac
      841 gatcatccgg tccggcacgt cgcgcaagac gatggcgccg gcgccgatga ggcaagccgc
      901 gccgatgcgc cggccctgga tcaccaccgc gctggcgccg acatgcgtct cctcgcccac
      961 gacgacgtcg ccgcacagca cggcgcctgg cgcgatatga gcgtggtcgc cgatgatgca
     1021 gtcatgatcc accgccgcgt gcgtattgat gatcgcgttg gcaccgatgc gcgtccccgg
     1081 ctgcaccacc gcccccgcca tgatctggca gccctcgccc agcgagatgc ccgccagcgc
     1141 gatcgtcgcc gccggatgcg ccacctgcgc gaagctgtat cccgccgctt tcatcttctc
     1201 gaagacgcgc cggcgcgaga cgtcgccgat gctgcccact ccattgacaa gccgataccc
     1261 gtccggcgga tagctcgcga gcgcctcgtc gccgcccagc cacggcacgc cctcgggcag
     1321 cgccgcagtc accccctgcg ccggatcgat cacgcccgtg acggcgacgc ccgcgcggcg
     1381 cagcgcctcg atgacgacac ggccatgacc gccgccgccg atgacgatca cgggaaggct
     1441 cacagcagga tcggctcgtc ggccgcgtag tcgcggtcgg ctgtccgctc cagcgcttcc
     1501 caatagcgca tcgccgcgac gccgcccggc gcgcgcttcg tcgtgagatt ctccgccgtg
     1561 aaggcttcgc cgcggccgat cgcccgcgcc gccaccaggc atttgcgcgc caccgcccgc
     1621 atggcgatct cgcccgcgac cggcgctttg cgcgggctgc ccagcgcggc ttccgtctcg
     1681 cggatcgccg ccaccatgcg gcgcagctcg tcgggctcga gcgacgcctg gtgatcgggg
     1741 ccgggcagcg agcggtcgag cgtgaaatgc ttctcgatca tcaccgcgcc gcgcgccgcc
     1801 gccgcgacgg ccacggtcgt gcccagcgta tgatcggaat atccgacggc aaggccgaag
     1861 gcgccgtgca gcgtgtccat cgcgcggaga ttcaccgtcg ccgccggcgc cggatactgg
     1921 ctcgtgcagt gcagcagtgt gacgcgcgcg tgcagcgcct gttggcccgc cgcgctcgca
     1981 taggctgctt cgaacgccgc ttcgcccggc agcgccgccg gatccgtcag gccgaacgcc
     2041 accacgccca gcgccgcttc cacctcgccc agatccgcca tgcccgtcga caggatcagc
     2101 gccgcgccgc tgcgcgcggc gtggagaaga agcggcccgt tggtgagatc gcccgacgag
     2161 atcttgacgc gcgccagcgc gtggcgccgc accagcaggt cgacgctcgg caggtcgaag
     2221 ggtgtcgaca ggaactcgat gccgcgctcg gcgcaatagg gcacgagcgc cgcatgggcc
     2281 gcctcgtcga gctcgagccg ctccagcatc gcatgctggc tctcggcggc gccgtcgctc
     2341 ttctgatact cggccttggc ggccgcacgc gtcgcgatgc ggtcggcgcg gaaggtct
//
LOCUS       DASYNS010000232         8284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2549001, whole genome shotgun sequence.
ACCESSION   DASYNS010000232 DASYNS010000000
VERSION     DASYNS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8284
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2549001"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VGP50_07270"
     CDS             complement(<1..301)
                     /locus_tag="VGP50_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV8027214.1"
                     /translation="MTATLAFSDAHIGSQPQRGESLSALDRIGTAMSLARGETLFYEG
                     DAALSCFKVISGALRSCRVLADGRRHIAEFFLPGDFIALSAENAHRFTAEAVTETK"
     gene            complement(435..809)
                     /locus_tag="VGP50_07275"
     CDS             complement(435..809)
                     /locus_tag="VGP50_07275"
                     /inference="COORDINATES: protein motif:HMM:NF018228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8027215.1"
                     /translation="MLPSPTRIFIVDDDDAVRDSLRMLLESYGMEVEDFASTGELARH
                     YRPGSGQCLVLDQHLPNLSGLDFLASRQGAMLKIPVILVTGRGDASIRERAHALGVLE
                     YLEKPVSEERLLAAIAKALGTD"
     gene            complement(891..2381)
                     /locus_tag="VGP50_07280"
     CDS             complement(891..2381)
                     /locus_tag="VGP50_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8027216.1"
                     /translation="MGDVEQDRLLRTLLDEAAIGIKWVASDGRLVEANLSLCRLLGYE
                     RAELLQRRDDEITHPDDQEGDQVLFARLLRGEIGSYSLEKRYLTRSGHALPVRVTSAL
                     TRTDDILRLGIVEDMRQRHEGEVTLRSILNTVPDAMVIISERGIIESFSPSAERLFGY
                     QAAEVVGKNVKVLMPAPYRERHDSYIENYVETGERRIIGIGRIVTGLRKDGTTFPLEL
                     SVGEAVIAGRRIFTGFVRDLSEREEAERRLERLQQELMHVGRLSEMSQMGSALAHELN
                     QPLSAINNYLRAGTRMLEAMGIAKDERVFEVMDKAGAQARRAGEIIRGLRQLVEKHET
                     ERRDEDVNKIVEEASALALLGAKNGGITVRIELSEQRLTALVDRIQIQQVVVNLVRNA
                     VEAMASSPEKRLTIATRREGDSVTASVSDSGPGLRSDIADRLFMPYVTSKEKGMGIGL
                     SICRQIIDAHGGRIGVETIPTGGTRFFFTLPLSGRPSESPSQTPAA"
     gene            2528..2704
                     /locus_tag="VGP50_07285"
     CDS             2528..2704
                     /locus_tag="VGP50_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027217.1"
                     /translation="MEMRADMLRRRVALYRDYLSKGVESGLARQYLQAIAEAEGELAK
                     LREREQRVPPRTTA"
     gene            complement(2723..2980)
                     /locus_tag="VGP50_07290"
     CDS             complement(2723..2980)
                     /locus_tag="VGP50_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027218.1"
                     /translation="MSRPRLASLPPVAAVAILTLSIGGCVEPPPSYYSSYATAPAAVP
                     PAAPAVPECREVHLTVTIGNQPQDAVSTACRQPDGTWRIRG"
     gene            3290..3736
                     /locus_tag="VGP50_07295"
     CDS             3290..3736
                     /locus_tag="VGP50_07295"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEV8027219.1"
                     /translation="MVKRPVTWYLVADGSRARVVSCLSMESNCEVVASYESDEAHLPD
                     RELTSDRPGRGQESATTGRHALEPRHDPHRGRKEAFIRQVATHLKDANGRAAFDALVL
                     YADPRSLAVLRDSLDDSTRAKVKRSVAKDLTKIPLAELGRHFAAES"
     gene            3766..4188
                     /locus_tag="VGP50_07300"
     CDS             3766..4188
                     /locus_tag="VGP50_07300"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV8027220.1"
                     /translation="MEPRTDSAAPEGEKPRESLFADLLSQLFGEAETLLLQEFALLRA
                     ELAENAGRFVGGIFVILAGMLVVLIGAIGIVAAATLLLGSYIPMWLACALVGAVVIVL
                     GVLLGLYGRRLLARATLTPERTLQSLRETASWIREELT"
     gene            4185..4622
                     /locus_tag="VGP50_07305"
     CDS             4185..4622
                     /locus_tag="VGP50_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027221.1"
                     /translation="MSGRREVSAAEIAEAIAKSRARLAHTLAVLDREYALRHLFVRGT
                     RMLRHYGDPGGATLGERMRGDILPVAVLGIGLAWLTLVGRRDGAQLGRRLIEGVAHLQ
                     ALARALVAFAAAPPGDGAAQGEASAAAPADRPPNPASTLTPDS"
     gene            complement(4606..5415)
                     /locus_tag="VGP50_07310"
     CDS             complement(4606..5415)
                     /locus_tag="VGP50_07310"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV8027222.1"
                     /translation="MRGPPWPRGRLPASATVVAAHPDDEVIGAGALLARLPQSLVVHV
                     TDGAPRNGVDARAAGFAGWAEYAAARHREAAAALALAGLPAERLRCFDIPDQEATLNL
                     VKLARRIAGLLREAGSPAVITHAYEGGHPDHDATAFAVHAAQHLLAQAGGDPPLLIEM
                     AGYHGFGGAFVVGSFIAHAGSGRIATMTLGAAAQARKGRMLDCHVSQRAVLAPFSVAA
                     EHFRLAPRYDFSLPPHPGALHYERQEWGMSGERWRTLAREAVLELGLKNPV"
     gene            5574..7025
                     /locus_tag="VGP50_07315"
     CDS             5574..7025
                     /locus_tag="VGP50_07315"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HEV8027223.1"
                     /translation="MSVISMAVLRPASSSASDELRSTLERQRSAFLKSAPPGLAERRA
                     DLAKLGKAIRESAPRLAEAISADFGNRSRHETMLAEVFTTLAGIRHATHHLPRWMKPK
                     RVSVSLELMPAQARILYQPVGVVGIISPWNYPFQLAMMPLLAALAAGNRVMLKPSELT
                     PRTGEFMAELLAGLFPSEQVATVLGGPEVGAAFSRLPFDHLFYTGSTAVGRLVMQAAA
                     ENLTPVTLELGGKSPCIIGEDAALAGAAESVAYGKLLNAGQTCIAPDYVLVPEAQREA
                     FINLLGQAVRKLYPTLKANPDYTSIVNERHYRRLAQYLDEAKAKGSRVVELNPASETL
                     DTGARKLAPTLVVDPAEDLALMRDEIFGPILPIKTYRGLDDAIDYVNRRPRPLALYYF
                     GADAAKRDDVLARTISGGVSVNETLMHIVVENLPFGGVGASGIGAYHGEIGFQTFSHR
                     KGVFLQSRINGSWMLRPPFGKLTDLMLKVLLRR"
     gene            complement(7026..7469)
                     /locus_tag="VGP50_07320"
     CDS             complement(7026..7469)
                     /locus_tag="VGP50_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEV8027224.1"
                     /translation="MPVLSLLLNVLWIFCGGLWMAAGWVMAAVIMALTIVGLPWARAA
                     FNIALYAFLPFGYRAVSRAEYRGRADLGTGPLGVLGNILWFILAGWWLALAHLLHAVV
                     LAITIIGLPFAWAHLKLAGLALWPIGKMIVPVEDDRPYGRGFPAR"
     gene            complement(7498..7893)
                     /gene="pspC"
                     /locus_tag="VGP50_07325"
     CDS             complement(7498..7893)
                     /gene="pspC"
                     /locus_tag="VGP50_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HEV8027225.1"
                     /translation="MTWTNSSGNPFRLYRNTERGILAGVCAGIADYLGIEPIVIRMAM
                     VGGLVFFLFPTIVAYIVLAVLLKPKPAALFRNGEEEQFWRGVSTAPSDTFQSLKHKFQ
                     ELEDRLRQMESQVASGDFDLHRKFRDLDR"
     gene            complement(7898..8137)
                     /gene="pspB"
                     /locus_tag="VGP50_07330"
     CDS             complement(7898..8137)
                     /gene="pspB"
                     /locus_tag="VGP50_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HEV8027226.1"
                     /translation="MFIGGGVFVLGIIFLTVVAPIWIIAHYVTHWRAARRLSAGDEKA
                     LGELWQIAQRMDGRIQTLERILDNEAPGWRMRAGG"
BASE COUNT         1370 a         2759 c         2785 g         1370 t
ORIGIN      
        1 gcttcgtctc cgtcacggct tcggcggtga agcggtgagc attctcggcg ctcagcgcga
       61 tgaagtctcc cggcaggaag aactcggcga tgtgccgtcg tccatcggcg agcacgcggc
      121 agctgcgcag ggcgcccgag atcaccttga agcaagagag ggccgcatcc ccttcgtaga
      181 acagcgtctc gccccgggca agcgacatcg ccgtgccgat ccgatcgagc gccgacagcg
      241 attcgccgcg ttgcggctgg ctgccgatat gtgcatccga gaaggcgaga gtcgcagtca
      301 ttggggcatc tccgtcgaag acagtcgaac ctgaaattcg ccaggacagt cgcgcatcgg
      361 ggtgcggcgg aacagttcgg atttctacgt aaggggttct acttatgcgg tcccggcggt
      421 gccggaggcc gcggctaatc ggtgccgagg gccttggcga tggccgccag cagacgctcc
      481 tcggagacgg gcttttcgag atattccaac acgcccagcg catgggcgcg ctcgcgaatg
      541 gacgcatcgc cgcggcccgt caccaagatc accgggatct tgagcatggc gccttggcgc
      601 gaggcgagga aatcgaggcc gctgagattg ggaaggtgct ggtctagaac cagacattga
      661 ccactccccg gacgatagtg gcgtgccaac tcccctgtgg aggcaaagtc ctcgacctcc
      721 atgccgtagg attcaagcag catcctgagg gaatccctca cggcatcgtc gtcgtcgacg
      781 ataaatatcc tggtgggcga tggcagcatg cgggtatccg caggaactcc caagtgagcc
      841 tcgcggaacc ataatcccgc cctgccgggt ggtaatatac gcaataatcc ttacgcggcg
      901 ggcgtttggc tggggctctc ggaaggccgg ccggaaagcg gcaatgtgaa gaagaaccgc
      961 gtcccgccgg tcggaatggt ctcgaccccg atgcgtccgc catgggcgtc gatgatctgc
     1021 cggcagatcg acaggccgat tcccatgccc ttttccttgc tcgtgacgta gggcatgaac
     1081 aaccggtcgg caatgtcgct gcgcaggccc ggcccgctat cgctgacgct tgccgtcacg
     1141 ctgtctccct cgcggcgcgt cgcgatcgtc agccgctttt ccggcgagga tgccatggcc
     1201 tcgacggcgt tgcgcacgag attgacgacg acctgctgga tctggatccg gtcgacgagg
     1261 gccgtgagcc gctgctcgct gagctcgatg cggaccgtga tgccgccgtt cttggcgccg
     1321 agcaaggcga gcgcgctcgc ctcctcgacg atcttgttga cgtcctcgtc gcgtcgctcg
     1381 gtctcgtgct tttcgacgag ttgccgcagg ccgcggatga tctcgccggc gcggcgcgcc
     1441 tgggcgccgg ccttgtccat cacctcgaag acgcgctcgt ccttggcaat acccatcgcc
     1501 tccagcatgc gcgtcccggc gcggagatag ttgttgatcg ccgacagggg ctggttgagc
     1561 tcgtgggcaa gcgccgagcc catctggctc atctcgctca gccggccgac atgcatcagc
     1621 tcctgttgca ggcgctcgag ccggcgctcg gcctcttcgc gctcggagag gtcgcggacg
     1681 aagccggtga agatccgacg accggcgatc accgcctcgc cgaccgacag ctcgagcggg
     1741 aaggtggtgc cgtccttgcg cagaccggtg acgatccggc cgatgccgat gatccgccgc
     1801 tcgccggtct cgacgtaatt ctcgatatag gagtcgtggc gctcgcgata gggcgcaggc
     1861 atgaggacct tgacgttctt cccgacgacc tcggccgcct ggtaaccgaa caggcgctcg
     1921 gccgacgggc tgaaggactc gatgatgccg cgctcgctga tgatgaccat ggcgtccggc
     1981 acggtgttca gaatggaacg cagcgtcacc tcgccctcat ggcgctggcg catgtcttcg
     2041 acgatgccga ggcggaggat gtcgtcggtc cgcgtgagcg cggacgtcac gcggacgggt
     2101 agggcgtggc cgctcctggt cagatatcgc ttctcgaggg aatagctgcc gatctccccg
     2161 cggaggagcc gggcgaacag cacctggtcg ccctcctggt catcgggatg cgtgatctcg
     2221 tcgtcgcggc gctgcaagag ctcggcgcgc tcatagccga gcaagcggca caggctgaga
     2281 ttggcttcga cgagccgacc gtccgacgcc acccatttga tgccgatggc cgcttcgtcc
     2341 agcagcgttc gcagcagccg atcttgttca acgtcgccca caacccgctc cagccgcgac
     2401 cattcgcgac gaatttatct tataccacaa gcggatcggt gaagcgcccg gcggcggacg
     2461 gcgggcaaat cttcgccaat ttgatctggc tcatggcggt cctccccgga cagggcatga
     2521 tcaacggatg gaaatgcgcg ccgatatgct ccgccgccgg gttgctctct atcgggatta
     2581 tctgagcaag ggcgttgagt ccgggctcgc ccggcaatat ctgcaggcga tcgccgaggc
     2641 cgagggcgag ctggcgaagc ttagggagcg cgagcagcga gtcccaccac gaacaacggc
     2701 ttgagccggg ttcggaacag tttcagcctc tgattcgcca agtgccgtcg ggctgccggc
     2761 aggcggtgct gaccgcgtcc tgcggctggt tgccgatcgt gaccgtcaaa tgcacctcgc
     2821 ggcattcggg gacggccggt gccgccggag gtacggccgc cggagcggtg gcataggagg
     2881 aatagtagga cggtggcggt tcgacacagc cgccgatcga cagcgtaaga atggcgactg
     2941 ccgcgaccgg cggcaaactg gcaaggcgag gtctgctcat cgcggcctcc atgaagttta
     3001 ggcgacacta tagggctctg cggcggccgg tcacagcaat gttcgaccct tgtccctccg
     3061 cgccggtgga gacctgcgcc tttccgcgcc atccggcgga ttgaggtaca tcaattcttt
     3121 gctactgcga gcactgtagc ttcagcggtt cgtcagcgtc ggcggtaaag gggagcatgg
     3181 tcgctaatat cgctgttccc tcggtgaagg tcgggcgcac atcgccggcc ggcgccgccc
     3241 gcggccgcgg cgtcgctgac gccacaatcc cgctcaagag gtcgatacaa tggtcaaacg
     3301 ccccgtgaca tggtacttgg tcgccgacgg cagccgcgcg cgcgtcgtca gctgtctctc
     3361 gatggagtcg aactgcgaag tggtcgcgtc ctacgaatcg gatgaagcgc atctccccga
     3421 tcgcgagctg acaagcgacc gtccgggccg tggccaggag agcgccacga ccggccgcca
     3481 tgcgctggag ccgcgccacg acccgcatcg cgggcgcaag gaagccttca tccgccaggt
     3541 cgccacgcat ctgaaggacg ccaacggacg tgccgccttc gatgcgctgg tcctctatgc
     3601 cgatccgcgc tcgctcgcgg tcttgcgcga cagcctcgat gactcgacgc gcgccaaggt
     3661 gaagcgcagc gtcgccaaag atctcaccaa aataccgctc gcggagctgg gccgtcattt
     3721 cgcggcggaa tcctgagttc cgcgagcgat ctcacggagc agcccatgga gcctcgaacc
     3781 gattccgccg cgcccgaggg cgaaaaaccg cgcgagtcgc tcttcgccga tcttctctcg
     3841 cagctgtttg gagaagccga aaccctgctg ctgcaggaat tcgcgctgct ccgcgccgag
     3901 ctcgccgaga atgccggccg cttcgtcggc ggcatcttcg tcatcctggc cgggatgctt
     3961 gtcgtgctca tcggcgcgat cggaatcgtc gccgcggcga cgctgctgct tggctcttac
     4021 atcccgatgt ggctcgcctg cgctctggtc ggtgccgtgg tgatcgtcct cggtgtgctg
     4081 ctcggtcttt acggccggcg tctcctcgcg cgcgcgacgc tgacgcccga gcgcacattg
     4141 cagtcgctgc gcgaaaccgc cagctggata cgcgaagagc tgacatgagc ggccggcggg
     4201 aagtctcggc cgccgagatc gctgaagcga tcgccaagag ccgcgcccgc ctcgcgcaca
     4261 cgctcgccgt gctcgaccgt gaatacgcgc tgcgccacct ctttgtgcgc ggcacgcgga
     4321 tgcttcgcca ttacggagat cccggcggcg cgacgctcgg cgagcggatg cgcggcgaca
     4381 ttcttcccgt ggcggtcctc ggcatcggtc tggcctggtt gacgctggtc ggccggcgcg
     4441 acggtgccca gctcgggcga cggctcatcg agggcgtcgc ccatctgcag gcgctggcgc
     4501 gcgccctcgt cgcgtttgcg gcggcaccgc caggtgacgg cgccgcgcag ggcgaggcct
     4561 cggccgcggc gccggccgac cgcccgccga atcccgcatc gacactcaca ccggattctt
     4621 gaggccgagc tcgagcaccg cttctcgcgc cagggtgcgc cagcgctccc cgctcatccc
     4681 ccattcctgg cgctcgtagt ggagtgcgcc ggggtggggc ggcaggctga aatcgtagcg
     4741 cggcgccagg cggaaatgct ccgctgcgac gctgaacggc gccagcacgg cgcgctggct
     4801 gacatggcaa tcgagcatgc ggcccttgcg cgcctgcgcc gcggcgccga gcgtcatcgt
     4861 ggcgatgcgc ccggaaccgg catgggcgat gaagctgccg accacgaagg cgccgccgaa
     4921 gccgtgatat ccggccatct cgatgagaag cggagggtcg ccgccggcct gcgccagcaa
     4981 atgctgcgcc gcatgcaccg cgaaggccgt cgcgtcgtgg tcgggatggc cgccctcata
     5041 ggcgtgggtg atcaccgcgg gtgagccggc ctcgcgcaag agcccggcga tgcgccgcgc
     5101 cagcttcacg agattgagcg tcgcttcctg atcgggaatg tcgaagcagc gcaaccgctc
     5161 ggcgggaagc ccggccagcg ccaaggcggc tgcggcctcg cggtgccgcg ccgcggcgta
     5221 ttcggcccag ccggcaaagc cggccgcgcg cgcatcgacg ccgttgcgcg gcgcgccgtc
     5281 cgtgacatgg acgaccagcg attgcggcag gcgcgccagc agcgcgcccg cgccgatgac
     5341 ttcgtcgtcg gggtgggccg cgacgacggt ggcggatgcc ggcaggcggc cgcgcggcca
     5401 gggcggaccc ctcattgccg aaagatgcgg cagcgaaagt cgagccgcaa gccggtacac
     5461 gcatttggga agcgatgccg cttgccccgg ccccggtcgt gcccctacat gattggcgag
     5521 gttggcccgg ccagccggac agaactcggc gcggggaccc agggaggacg gtcatgagcg
     5581 tcatcagcat ggcggtgctg cggccggcat catcgagcgc cagcgacgag ctgcgctcta
     5641 cgctcgagcg ccagcgctcg gcgttcctca agagcgcgcc gccgggcctc gcggagcgtc
     5701 gtgccgatct ggcgaagctc gggaaggcga tccgcgagag cgcgccccgg ctggcggagg
     5761 cgatctccgc cgatttcggc aaccgctcgc ggcatgagac gatgctcgcc gaggtgttca
     5821 cgacgcttgc cggcatccgc cacgccacgc accacctccc gcgatggatg aagcccaagc
     5881 gcgtgtcggt gagcctcgag ctgatgccgg cgcaagcacg catcctctat cagcccgtgg
     5941 gcgtcgtcgg catcatcagc ccctggaact atccctttca gctggcgatg atgccgctgc
     6001 tcgcggcgct cgctgccggc aatcgcgtga tgctgaagcc gtcggagctg acgccgcgca
     6061 cgggcgaatt catggcggag ctgctcgccg gcctcttccc cagcgagcag gtggcgacgg
     6121 tgctcggcgg ccccgaggtc ggcgcggcat tctcgcggct ccctttcgat catctcttct
     6181 acacgggatc gacggcggtc gggcggctcg tcatgcaggc ggccgccgag aatctgacgc
     6241 cggtgacgct ggagctcggc ggcaagtcgc cctgcatcat cggcgaggac gcggcgctcg
     6301 ccggcgccgc cgagagcgtt gcctatggca agctgctcaa tgccggccag acctgcatcg
     6361 cgcccgacta tgtgctggtg cccgaggcgc agcgcgaagc cttcatcaat ctgctcgggc
     6421 aggcggtgcg caagctctat ccgacgctca aggccaatcc cgactacacc agcatcgtca
     6481 acgagcgtca ctatcgacgt ctcgcgcaat atctcgacga ggcaaaggcg aagggtagcc
     6541 gcgtcgtcga gctcaacccg gcgtcggaga cgctcgacac gggcgcgcgc aagctcgcgc
     6601 cgacgctggt ggtcgatccc gccgaggacc tcgcgctgat gcgcgacgag atcttcggcc
     6661 cgatcctgcc gatcaagacc tatcgcggcc tcgatgatgc catcgactat gtcaaccggc
     6721 ggccgcggcc gctcgcgctc tattatttcg gcgccgacgc ggcaaagcgc gacgacgtgc
     6781 tggcccgcac gatctcgggc ggcgtgtcgg tcaacgagac gctcatgcac atcgtcgtcg
     6841 agaatctgcc cttcggcggc gtcggcgctt ccggcatcgg cgcctatcac ggcgagatcg
     6901 gcttccagac cttctcgcac cggaagggcg tgttcctgca aagccgcatc aacggctcat
     6961 ggatgctgcg cccgcccttc gggaagctca ccgatctcat gctcaaggtg ctgctgcggc
     7021 gctgatcagc gcgccggaaa tccccgtcca taggggcggt catcctcgac cggcacgatc
     7081 atcttgccga tcggccagag ggcgaggccc gccagcttca gatgtgccca ggcgaagggc
     7141 aggccgatga tggtgatggc gaggaccacg gcatggagca gatgggcgag cgccagccac
     7201 catcccgcga gaatgaacca gagaatgttg ccgagcacgc caaggggtcc ggttcccaga
     7261 tccgcgcgtc ccctatattc ggcgcgtgac accgcgcggt agccgaacgg cagaaaagcg
     7321 tagagcgcga tattgaaggc ggcgcgcgcc caggggaggc cgacgatggt gagcgccatg
     7381 atcacggccg ccatcaccca tccggcggcc atccacaggc cgccgcagaa aatccacaga
     7441 acattgagca ggagcgacag cactggcatt gggaatctcg cgcgtgaggg aaagaggtca
     7501 gcgatcgaga tcgcggaatt ttcgatggag gtcgaaatcg cccgaagcga cctggctctc
     7561 catttggcgc agccggtcct ccagctcttg gaatttgtgc ttgagcgact ggaaggtgtc
     7621 gctgggcgcc gtgctgacac cgcgccagaa ttgctcctcc tcgccgttgc ggaagagcgc
     7681 cgccggcttc ggcttcagca gcaccgcgag cacgatgtag gcgacgatcg tcggaaacag
     7741 gaagaagacg agcccgccga ccatcgccat gcggatgacg atcggctcga tgccgagata
     7801 atcggcgatg ccggcgcaga cgccggccag gatcccgcgc tcggtattgc ggtagaggcg
     7861 gaaggggttg ccgctgctgt tggtccaggt catggggtca gcctcctgcg cgcatgcgcc
     7921 agcccggcgc ctcgttgtcg aggatgcgtt cgagcgtctg gatccgcccg tccatgcgtt
     7981 gcgcgatctg ccagagctcg cccagggcct tctcgtcgcc ggccgagagc cgccgcgcgg
     8041 cgcgccaatg ggtgacgtaa tgggcgatga tccagatcgg cgcgacgacc gtcaggaaga
     8101 tgatgccgag gacgaaaact ccgccgccga tgaacatggg gtcctatccc ttcgccgggg
     8161 tcggacggat gctgttgccg agtcgctcct tcaacgcctt cagctcttcc tcgacgccat
     8221 cttccgcctc catcccggcg aattcgtcgg ccagcgtttt cttgcgtccg agatcgaagg
     8281 cctc
//
LOCUS       DASYNS010000233         3583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4265794, whole genome shotgun sequence.
ACCESSION   DASYNS010000233 DASYNS010000000
VERSION     DASYNS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4265794"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VGP50_07335"
     CDS             <1..574
                     /locus_tag="VGP50_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HEV8027227.1"
                     /translation="RVLTQLPEAFATEVILRMLRMEAVQKDVLDDVERTLRNEFFSSL
                     ARTNRRDSHELVAEIFNGLDRSTEQRFVAAIDERSHDSAERIKALMFTFEDLGRLDAT
                     AIQAMLRAAEKDKVPLALKGASETLRDLFLGGMSERAGKLMKEEISSMGPVRLMDVEE
                     AQSYLVQLAKELAARGEIIIAESEEDQLVY"
     gene            574..1236
                     /locus_tag="VGP50_07340"
     CDS             574..1236
                     /locus_tag="VGP50_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HEV8027228.1"
                     /translation="MSTPHKFLFDRSFDQAEGPPGVSRRPPPEPPVTRADVEAARAQG
                     VAEGRKAALAEAARSAEERLATALSALAAGMKDLIATREQESAQLQRRSLDAFRAITR
                     KAVPALCRKDPLAEVEALVVDCLHEAFDEPRLVLRVAASLFPELRERVEQLAASTGFA
                     GKVVLLADETLGPGDARIEWADGGAERDGARLLSDIDAALARAIDAISNQPTKPLEES
                     KT"
     gene            1233..1565
                     /gene="fliN"
                     /locus_tag="VGP50_07345"
     CDS             1233..1565
                     /gene="fliN"
                     /locus_tag="VGP50_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEV8027229.1"
                     /translation="MTDTQNPQNLPLDELEQTPPSNADAKPQTAKDLEAVYEIPVQIS
                     AVLGRATMQVSQLLKLGRGAVVELDRKVGEAIDIYVNNRLVARGEVVVVDERLGVTMT
                     EIVKADRS"
     gene            1591..2931
                     /locus_tag="VGP50_07350"
     CDS             1591..2931
                     /locus_tag="VGP50_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV8027230.1"
                     /translation="MRLLIVGPIEGYISAAGKIAIARGAAVAHVDNIERALAALRAGE
                     GADLLMMDVKLDIGRMVESLRQERINIPVIACGVAADVGAAVRAIKAGAKEYIPLPPD
                     ADLIAAVLEAVAQESHALLYRDPVMAEVVRLAEQVAPSDASVLITGESGTGKEVIARY
                     IHRKSRRAKQNFISVNCAAIPENLLESELFGHEKGAFTGAVARRIGKFEEASGSTLLL
                     DEISEMHPRLQAKLLRAVQEREIDRVGGTQPIKVDIRLLATSNRDLEEEVRAGNFRED
                     LYFRLNVVGLRMPALRERPKDIPLLADHFAKKYAEMNGLAVRALAPGVHQMLAQHGWR
                     GNVRELENALHRAVLLARGDEIGPEAIMLSSAKSPAAADAAATAGKVGLVGRTVADVE
                     RDLILETLQHCLGNRTHAANILGISIRTLRNKLQQYRQEGVAVPHPGEPERVRA"
     gene            3006..>3583
                     /locus_tag="VGP50_07355"
     CDS             3006..>3583
                     /locus_tag="VGP50_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HEV8027231.1"
                     /translation="MADTTAPAPLTAADLLERLGATLRRGEIALALGVVAILSVLILP
                     LPSWLLDISLALSITFSVLILMVALFIQRPLEFSSFPTVLLIATMLRLSLNLASTRLI
                     LSHGHEGTDAAGHVIQAFGTFVMAGNFIIGVIVFTILVVVNFVVITKGSGRIAEVAAR
                     FSLDSMPGKQMAIDADLSAGLINEDTARKRRT"
BASE COUNT          601 a         1271 c         1167 g          544 t
ORIGIN      
        1 gcgcgtgctg acgcagcttc cggaggcctt tgccaccgag gtgatcctgc gcatgctgcg
       61 catggaggct gtgcagaagg acgtgctcga cgacgtcgag cgcacgctgc gcaacgagtt
      121 cttctcgagc ctcgcccgca ccaaccggcg cgactcgcac gaactggtcg ccgagatctt
      181 caacgggctc gatcgcagca ccgagcagcg cttcgtcgcc gcgatcgacg agcgcagcca
      241 cgattcggcc gagcggatca aggcgctgat gttcaccttc gaggatctgg ggcgcctcga
      301 cgctacggcg atccaggcga tgctgcgcgc cgccgagaag gacaaggtac cgctcgcgct
      361 gaagggggcg tccgagaccc tgcgcgatct cttcctcggc ggcatgtctg agcgcgccgg
      421 caagctgatg aaggaggaga tctccagcat ggggccggtg cgcctcatgg acgtcgagga
      481 ggcgcaaagc tacctggttc agctcgccaa ggagctggcg gcgcgcggcg aaatcatcat
      541 cgccgagagc gaagaagacc agctcgtgta ctaatgagca ccccgcacaa attcctcttc
      601 gaccgctcct tcgaccaagc cgaaggaccg cccggcgtgt cgcgcaggcc gcccccggag
      661 ccgcccgtga cgcgggccga cgtcgaggcg gcgcgggcgc aaggcgtcgc cgagggacgc
      721 aaggcggcgc tcgccgaggc ggcgcgctcc gccgaagagc gcctcgcgac agcgctttcg
      781 gcgcttgccg ccggaatgaa ggacttgatc gcgacccgcg agcaggagtc ggcacagctt
      841 cagcgccgct cgctggacgc gtttcgtgcc atcacgcgca aggcggtccc ggcgctctgc
      901 cgcaaggacc cgctcgccga ggtcgaggcg ctcgtcgtcg actgcctgca tgaggccttc
      961 gacgagccgc gcctggtgct gcgcgtcgcc gcttcgcttt ttcccgagct gcgcgagcgc
     1021 gtcgagcagc tcgccgcgtc cacggggttc gccggcaagg tggtgctgct ggcggatgaa
     1081 acgctcggcc caggcgatgc gcgcatcgaa tgggccgacg gcggcgccga gcgcgacggc
     1141 gcccgtctct taagcgatat cgacgcggcg ctcgcccgcg ccatcgacgc catttccaat
     1201 cagccgacca aaccgctcga ggagagcaaa acatgactga cacgcagaac ccgcagaacc
     1261 tgccgctcga cgaactcgag cagaccccgc cgagcaacgc cgatgcaaag ccgcagaccg
     1321 ccaaggacct cgaggccgtc tacgagattc ccgtccagat ctcggccgtg ctcgggcgcg
     1381 ccacgatgca ggtgagccag ctcctgaagc tgggccgcgg cgcggtcgtg gagctcgacc
     1441 gcaaggtcgg cgaggcgatc gacatctacg tcaacaaccg gctcgtggca cgcggcgagg
     1501 tcgtcgtcgt tgacgagcgc ctcggcgtca ccatgaccga gatcgtcaag gccgatcgca
     1561 gttaagcccc agaccacagg atcctttctg atgcgcctcc tcatcgtcgg cccgatcgag
     1621 ggctacatca gcgccgcggg caagatcgcc atcgcccgcg gcgcggccgt cgctcacgtc
     1681 gacaacatcg agcgggcgct cgccgcgctc cgcgccggcg agggcgccga cctcctgatg
     1741 atggatgtga agctcgacat cggccggatg gtcgagagcc tccgccagga gcgcatcaac
     1801 atcccggtga tcgcctgcgg tgtcgccgcc gacgtcggcg ccgccgtgcg cgcgatcaag
     1861 gccggagcca aggaatacat cccgctgccg cccgacgccg atctcatcgc tgccgtgctc
     1921 gaggccgtgg cgcaggagag ccacgcgctg ctctaccgcg atccggtcat ggccgaggtg
     1981 gtgcgcctcg ccgagcaggt cgccccttcc gacgcgagcg ttctgatcac cggcgagagc
     2041 ggcaccggca aggaagtgat cgcgcgctac atccatcgca agagccggcg cgccaagcag
     2101 aatttcatct cggtgaattg cgcggcgatc cccgagaacc tgctcgaatc cgagctcttc
     2161 ggccatgaga aaggcgcctt caccggcgcc gtggcgcggc gcatcggcaa gttcgaggag
     2221 gcgagcggca gcacgctgct gctcgacgag atcagcgaga tgcatccgcg cctccaggcg
     2281 aagctgctgc gcgccgtcca ggagcgcgag atcgatcgcg tcggcggcac gcaaccgatc
     2341 aaggtcgaca tccgcctcct cgctacctcg aaccgcgacc tcgaggaaga agtgcgcgcc
     2401 ggcaatttcc gcgaggatct ctatttccgc ctcaacgtcg tcggcttgcg catgccggcg
     2461 ctgcgcgagc ggcccaagga cattccgctg ctggccgatc actttgcaaa gaaatacgcg
     2521 gagatgaacg gcctcgccgt gcgtgccttg gcgccgggcg tgcatcagat gctggcgcag
     2581 catggctggc gcggcaatgt gcgcgaactc gagaacgcgc tccaccgcgc cgtcctgctc
     2641 gcccgtggcg acgagatcgg cccggaggcg atcatgctgt cgtcggcgaa atcgcctgct
     2701 gcggccgatg cggcggcgac cgccggcaag gtcggcctcg tcggccgcac ggtcgccgac
     2761 gtcgagcgcg acctcattct cgaaacgttg cagcattgcc tcggcaaccg gacgcatgcc
     2821 gcgaacatcc tcggtatctc gatccgcacg ctgcgcaaca agctccagca ataccgccag
     2881 gaaggcgtcg ccgtgcccca tcccggcgag cccgagcgcg tgcgcgcgta gcgcgtcgtc
     2941 acatgtcgcg cccggtcctc acccccgagc agaatccccc tctccccttg cgggagaggg
     3001 ttctaatggc cgacaccacc gcgcccgccc cactcaccgc ggccgatctc ctcgagcggc
     3061 tcggcgcgac gctccggcgc ggcgagatcg cgctggcgct gggcgtcgtc gccatcctgt
     3121 cggtgctgat cctgccgctg ccgtcctggc tcctcgatat aagcctcgcg ctctcgatca
     3181 ccttctccgt gctgatactg atggtagcgc tcttcatcca gcggccgctc gaattctcgt
     3241 cgttcccgac cgtgttgctg atcgcgacga tgctgcgcct ctccctcaac ctggcttcga
     3301 cgcggctgat cctcagccac ggccatgagg ggaccgacgc cgccggccac gtcatccagg
     3361 cgttcggcac cttcgtcatg gccggcaact tcatcatcgg cgtgatcgtc ttcacgatcc
     3421 tggtggtggt gaacttcgtc gtcatcacca agggctcggg gcgcatcgcc gaagtcgcgg
     3481 cgcgcttcag cttggacagc atgccgggca agcagatggc gatcgacgcc gacctttccg
     3541 ccggcctcat caacgaggac acggcgcgga agcggcgcac cga
//
LOCUS       DASYNS010000234         5615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1146730, whole genome shotgun sequence.
ACCESSION   DASYNS010000234 DASYNS010000000
VERSION     DASYNS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146730"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1124
                     /gene="rutD"
                     /locus_tag="VGP50_07360"
     CDS             324..1124
                     /gene="rutD"
                     /locus_tag="VGP50_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HEV8027232.1"
                     /translation="MPRISIGDCQLYYERCGAGFPVLFISGLSGYASYWREQVPSFAK
                     TFEVVLHDHRGIGQSDHSRISYTVERMATDVIQLMDALGIDKAHVVGHSTGGAVAQVL
                     ALEHPERVASIVIAASWTKADAYFRRLFALRKEILSHLGPAAYLQAATLLLYPSHWIA
                     RNNERLRQLEAQALATFSPPEIVMSRIDAILAFDRTEDLPRLRTPTLIVAAQDDIVTP
                     AYFSEELARLIRGAEIKLFPQGGHFFSQVLPREFNHAVLPFLTAHTPA"
     gene            1250..2956
                     /locus_tag="VGP50_07365"
     CDS             1250..2956
                     /locus_tag="VGP50_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HEV8027233.1"
                     /translation="MMPSEQNEDPQRLRATLTAAGDVAYEWDLVSDGITWLGSVEAML
                     GLREARAIVSGRTLAARLNPDDLLLRQQRLNAHWRHGATFDCEYRVRVENGGFAWVHD
                     RGTATFDEDGRPVRMGGVWRLITSRKAPEIRVEQAANYDELTGHFNKKRLREALDHVL
                     SSSLRCGSAGAYLAIGIDKLTAVKEALGVEVAEAVIVAISQRLDRSLRVSDVIGRVGG
                     DLFGIVLSNCAEEHIAAAAEKILSAVSHVPVMTTSGPVHVTVSVGSATFPEQAKTSYE
                     VMTRAEAALAEAKEAGRNCFVPSRLSEEQRDQYRLGLALGERVQRALNEDRLVFAYQP
                     VVDSRTGKVDYYECLLRMVAEDGRIIAAGEFMPIIEQLGFIRTIDRYVLERAVREVAA
                     HPGICLGFNISGLTATDYAWLRSITSMLKGKPEIARRLVVEITETAALHDIEESARFV
                     GALRELGCRVALDDFGAGFTSLRHLQALAVDTVKLDGSFVRNLAQNYDNQMFVRHLLG
                     LANGLGLDTVAECVETAREAAILRREGVGYMQGYYFAKPTIERPWSESDTDDVRHALA
                     AS"
     gene            complement(2967..4502)
                     /locus_tag="VGP50_07370"
     CDS             complement(2967..4502)
                     /locus_tag="VGP50_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEV8027234.1"
                     /translation="MADTITVETTVGRIRGESAGDLHIFRGIPYAAAPVGRRRFAAPA
                     PLARWSGVRDCTSFGARSIQPATGLDLAPEIGALLLLAPPPPMSEDCLFLNVWTPAPG
                     DGGKRPVMVWLHGGAFISGSGASPLYDGSNLARREDVVVVTLNHRLGLLGYLNLAELG
                     GEAFAASGNAGMLDIVAALEWLRDNIAGFGGDPGNVTLFGESGGGAKTSVLMAMPAAR
                     GLFHKAIIQSGPAVEMMSPADATAIARRTLEHLELDPRKPEALLESRVEDLLEAQGVI
                     LRSRGPLDFANRRKIGFNPVVDGRHLPQGPFAPTAPAISAGIPLMIGTNRDEMSLFFG
                     LAPWLEKPGEADLARLAERVLGERAPAVVAAYRRARPDASPRDLFIAITSDQGMRIPS
                     LVMAERKLAQRAAPVYVYLFTWETAVLDGRLKSCHALEIPFVFETVEKAARFTGGSAG
                     SLALARTMSRSWAAFARGGNPGHDGLPAWPVYSTERRPTMIFDERCRVEEDPLAEERR
                     AWA"
     gene            complement(4546..5154)
                     /gene="phaR"
                     /locus_tag="VGP50_07375"
     CDS             complement(4546..5154)
                     /gene="phaR"
                     /locus_tag="VGP50_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEV8027235.1"
                     /translation="MDDKVSAETPPVVIKKYANRRLYNTATSSYVTLDDLSRMVKAGR
                     EFVVYDAKTGEEITRPVLTQIIVEEEQKGQNLLPISFLRQLISLYGDSMQWLVPRYLE
                     HAMTTFARNQEQMRKNLQDAFGGLFPFGPLEEMGKQNLALFEKAMKMFSPFPAMPATD
                     KTRAGERPSGEPGAGDASVKELTDRLNALQQQIDALGKNRKE"
BASE COUNT          908 a         1859 c         1873 g          975 t
ORIGIN      
        1 acagaatttt ttggttcggc cgctcgatcg ctaacactcg ttgcgtgatg aggttccgct
       61 actacggcgc gacctccgat tgtcgccgcg cgtcgctcgc tgttccgaag gcgtcgcgat
      121 cgtcgtgtcg agcccttgca tcgtcgccca gcaggctcgt ttcgacgctg aagcgcgcgg
      181 gacgggattg tcgcgtgtca agtggccagg cccgcatccc tgccgctcgc gcatgactgc
      241 atatcctgcg cgatgtttga cagcagcggc cccgcaatgt taacgaccca caagctgtcc
      301 cgtcgaaacc agactcccgt actatgccgc gcatctcgat cggcgattgc caactttatt
      361 acgagcgctg tggagccggc ttcccggtgc tcttcatcag cggtctctcc ggctacgcct
      421 cctactggcg ggagcaggtg ccgagcttcg ccaagacgtt cgaggtggtg ctgcacgatc
      481 accgcggcat cggccagagc gaccacagcc gcatcagcta cacggtcgaa cgcatggcga
      541 ccgatgtgat ccagctcatg gatgcgctcg gcatcgacaa agcccatgtg gtcggccact
      601 cgaccggcgg cgcggtcgcg caagtgttgg cgctcgaaca tcccgagcgc gtcgccagca
      661 tcgtcatcgc ggcgagttgg accaaggccg atgcgtattt tcgccgactc ttcgccttgc
      721 gcaaggagat cctgagccat ctcggtccgg cggcctacct gcaggcggcg acgctgctgc
      781 tctatccgag ccattggatc gcgcgcaaca acgagcggct gaggcagctc gaggcgcagg
      841 cgctggcgac tttctcgccg cccgaaatcg tcatgagccg catcgatgcg atcctcgcct
      901 tcgatcgcac cgaggatctg ccgcggctgc gcacgccaac cctcatcgtc gcggcccagg
      961 acgacatcgt gacgccggcg tatttctcgg aggagctcgc ccgcctcatt cgcggcgcgg
     1021 agatcaaact cttcccgcaa ggcgggcatt tcttctcgca ggtgctgccg cgcgaattta
     1081 accatgccgt gctgccattc ctgaccgcgc acacaccggc gtgacggacg gcgatttacc
     1141 ggcttcccac ttaagctttc gtaaagtggt tggcttctac gatggctccc ggaatcacgg
     1201 gtcgaggccc tttcgtggcc ttcggccgga tcagcaggtg agccaccgga tgatgccctc
     1261 ggagcagaac gaggacccgc agcgcctgcg ggccaccctg accgccgcgg gcgacgtggc
     1321 gtatgagtgg gatctcgtca gcgacgggat cacgtggctc ggctcagtcg aggcgatgct
     1381 tggacttcgc gaggcgcggg ccatcgtctc gggccggacg ctcgccgcgc ggctcaatcc
     1441 cgacgacctg ctgctgcgcc agcagcgcct caatgctcat tggcggcatg gcgccacctt
     1501 cgattgcgag taccgcgtgc gcgtcgagaa cggcggcttc gcctgggtgc atgatcgcgg
     1561 caccgccacg ttcgacgagg acggccgtcc cgtgcgaatg ggcggcgtct ggcgcctcat
     1621 caccagccgc aaggcgccag agattcgcgt cgagcaggcc gcgaactacg acgagctgac
     1681 cgggcatttc aacaagaagc gattgcgcga ggcgctcgat cacgtgttgt cgtcgagcct
     1741 gcgctgcggc agtgccggtg cctatctggc gatcggcatc gacaagctga ctgcggtcaa
     1801 agaggcgctc ggtgtcgagg tggccgaagc ggtgatcgtc gccatcagcc aacgtctcga
     1861 ccgcagcctg cgggtcagcg acgtcatcgg ccgcgtcggc ggcgacctct tcgggatcgt
     1921 gctgtcgaat tgcgccgagg agcatatcgc ggcggccgcc gagaaaatcc tgtcggccgt
     1981 gagccatgtc ccggtcatga ccacctcggg acccgtccat gtcaccgtgt cggtcggcag
     2041 cgcgaccttc cccgaacaag ccaagacctc ctatgaggtg atgacccgtg cggaggccgc
     2101 gctcgccgag gcgaaagagg ccggccgcaa ttgcttcgtg ccctcccgcc tttccgaaga
     2161 gcagcgcgac cagtaccgtc tcggtcttgc gctcggcgaa cgtgtgcagc gcgcgttgaa
     2221 cgaggatcgt ctggtcttcg cctaccagcc cgtggtcgac agccgcaccg ggaaagtcga
     2281 ctactacgaa tgcttgctgc gcatggtggc cgaggatgga cgcatcatcg ctgccggcga
     2341 gttcatgccg atcatcgagc agctgggatt cattcgcacg atcgaccgct atgtcctcga
     2401 gagagcggtg cgggaagtgg cggcgcatcc cgggatctgc ctcggattca acatctcggg
     2461 cctcaccgcc accgattatg cgtggctgcg ctcgatcacc tcgatgctca agggcaagcc
     2521 ggagatcgcg cggcgcctgg tcgtcgagat cactgagacc gcggcgctgc acgacatcga
     2581 agagtcggcg cgcttcgtcg gcgcgctgcg cgagctcggc tgccgggtgg cgctggatga
     2641 tttcggcgcg ggcttcacct cgctgcgcca cttgcaggcg ctcgccgtcg acacggtcaa
     2701 gctcgacgga tcattcgtgc gcaatctggc acagaactac gacaaccaga tgttcgttcg
     2761 tcatctgctg ggcctggcca acgggctcgg cctggacacc gtggcggaat gcgtcgagac
     2821 ggcgcgcgag gcggccatcc tgcgccgcga aggcgtgggc tacatgcagg gctattactt
     2881 cgccaagccg acgatcgagc gtccctggag cgagagcgac accgacgacg tccgccacgc
     2941 gctggcagca tcctgaggcg cgcgcgtcaa gcccaagccc gacgctcctc cgccagcgga
     3001 tcctcttcga cgcggcaacg ttcatcgaaa atcatggtcg ggcggcgttc cgtcgaatag
     3061 acgggccagg ccggcaggcc gtcatgaccg ggattgccgc cgcgcgcgaa tgcggcccag
     3121 ctgcggctca tggtccgcgc caaggcgagg ctgccggcgg agccgccggt gaagcgcgcc
     3181 gccttctcca ccgtctcgaa gacgaaggga atctcgagcg cgtggcatga cttgagccgc
     3241 ccgtcgagca cggccgtttc ccaggtgaag agatagacgt aaaccggcgc cgcacgctgc
     3301 gccagcttgc gctcggccat gaccagcgaa gggatgcgca tgccctggtc ggatgtgatg
     3361 gcgatgaaga gatcgcgcgg cgaggcgtcg ggccgcgccc ggcgataggc ggcgacgacc
     3421 gccggcgccc gctcgccgag gacgcgctcg gcgagacgtg ccagatcggc ttcgcccggc
     3481 ttctcgagcc agggcgccag accgaagaac agcgacatct cgtccctgtt cgtgccgatc
     3541 atcaggggaa ttccggcgga gatcgccggc gccgtcggcg cgaagggccc ttggggcagg
     3601 tgtcgcccgt cgaccacggg gttgaagccg atcttgcgcc ggttggcgaa atcgagagga
     3661 ccgcgtgatc tgaggatcac gccttgcgcc tcgagcagat cctcgacacg gctctcgagc
     3721 agcgcttcgg gttttctcgg atcgagttcg agatgctcga gggtgcggcg ggcgatcgcc
     3781 gtcgcgtcgg cgggcgacat catctcgacg gcggggccgc tctggatgat ggccttgtgg
     3841 aagagtccgc gcgcggccgg catcgccatg agcacgctcg tcttggcgcc gccaccggac
     3901 tcgccgaaga gggtgacgtt gccggggtcg ccgccgaagc ccgcaatgtt gtcacgcagc
     3961 cattcgagcg cggcgacgat gtcgagcatg ccggcattgc ccgaggccgc gaaagcctcg
     4021 ccgccgagct cggccagatt gagatagccg agcaggccca agcgatggtt gagcgtgact
     4081 acaacgacat cctcgcgtcg cgcgagattg ctgccgtcat agagcggcga ggcgcccgat
     4141 cccgagatga aagcgccgcc atgcagccag accatgaccg gccgcttgcc gccatcgccc
     4201 ggcgccggcg tccagacgtt gaggaagagg caatcctcgc tcatcggcgg tggcggcgcg
     4261 agcaacagca gcgcgccgat ctccggcgca aggtcgagac cggttgcggg ctggatcgag
     4321 cgcgcgccga agctcgtgca atcgcgcacg ccgctccacc gcgcgagtgg tgcgggtgcc
     4381 gcgaagcggc ggcggccgac gggcgccgct gcatagggaa tgccgcgaaa gatgtgcagg
     4441 tcaccggcgc tctcgccgcg aatcctgccg acagtcgtct cgacggtgat cgtatcggcc
     4501 atcgggtctg ctccgtcgcc tctgggaata acctgcctcg cgcctttatt ccttgcggtt
     4561 cttgccgagc gcgtcgatct gctgctggag ggcgttgagg cggtcggtca gttccttgac
     4621 gctggcgtcg ccggcgccgg gctccccgga gggccgctcg cccgcgcggg tcttgtccgt
     4681 cgccggcatc gcggggaagg gcgagaacat cttcatggcc ttctcgaaca gcgccagatt
     4741 ctgcttgccc atctcttcca gcgggccgaa tgggaagagc ccgccgaacg cgtcctggag
     4801 attcttgcgc atctgctcct ggttgcgcgc gaaggtcgtc atggcgtgct cgagatagcg
     4861 cggcacgagc cattgcatgc tgtcgccata gaggctgatg agctggcgaa gaaagctgat
     4921 cggcaacagg ttctggcctt tctgctcctc ctcgacgatg atctgcgtga gcaccggccg
     4981 agtgatctcc tcgccggtct tggcgtcgta cacgacgaat tcccggccgg ccttaaccat
     5041 ccggctcaga tcgtcgagcg tcacatagct gctagtggcg gtattgtaga ggcggcggtt
     5101 ggcatatttc ttgatgacga ccggcggtgt ctcggcgctc actttgtcat ccattggacg
     5161 tgatctcccg gcgttctccc cgttcattcc cttcgacggc tgagcataac agctttctca
     5221 aagcgatgct aaacaggcgc agggctgctg gccttggggc gggaccgccg atatactccc
     5281 gaccccatgg cggaaccgcc ggaccttgcg accctggcca ggaggtattt gaacctgtgg
     5341 caggagcagt tgaccgccat ggcggcggat gccgatctcg ctcaggccct cgcccgcttg
     5401 ctggcgacgc tggcgccgtc cccccggccg gagcggccgg ccggtccgga tgaagggcgc
     5461 gccgccggcg gcacatcgac agggtccgcg gccgctggcg ctgcatctgg cgagcgcagt
     5521 cacggcgtgg acgagctcgc tggccgcgtt gccgctcttg agcaacgggt cgctgacctg
     5581 gaagccggag ctcgccgacg ccggccgcgc gctcg
//
LOCUS       DASYNS010000235         3364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2550585, whole genome shotgun sequence.
ACCESSION   DASYNS010000235 DASYNS010000000
VERSION     DASYNS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2550585"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VGP50_07380"
     CDS             complement(<1..477)
                     /locus_tag="VGP50_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027236.1"
                     /translation="MRTLRKALAGIGIGLLGLMLVVALVFGLAQTRPGKAALASLIER
                     WLSDPAERVSIDHIGGFVPFDMTVDAITVADSQGPRIVLSDTALAVAPADLLVGRLTL
                     VRIAARALRIERGPQSASGGSDLAALLHPPLAVTIERLQLDRIELGRDLLGEPATLT"
     gene            complement(491..2308)
                     /locus_tag="VGP50_07385"
     CDS             complement(491..2308)
                     /locus_tag="VGP50_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HEV8027237.1"
                     /translation="MTSPQRDHPFAARPLWPLAILVAAFLVLWHASPATSDISYSSEI
                     HLEGVKDSALERTLRDVSQLVKLEDKPPPSEAALRRRAEDDLPRLKEVVQAAGYWTPQ
                     LSYRLEPKDDSVTVEVIVDPGPLFHLESATFHGTSGEALPLLDQLGSAALGLTIGEPA
                     RSAPVGAAEQQIVETYARNGHPFAKVVEHRAVVDVAKHTMSVTYTIDPGPEAHFGPVT
                     ISGLEHVDRAYVEARIAWREGALYDARLVEKTRQGLVKSGLFSAARINHSDAPDGAGQ
                     VAMTVDLVEGPPRSVGAGVAYNTNLGAGGQIFWEHRNLFGGAENLRITAAVAQRQDGL
                     ALAFRKPDFLDRNQELLANAALLQQNTDAYRSLREQAFLGLERPLLPSITVDGGVSFE
                     HANVQSDVFGTEDYTLLGLPFLLRRDTTDDLLDPTLGSRQTLTLIPYHSLSGPNLNFV
                     NSRIEERDYERLNDSGRLVLASFAAFGSIFGATRDEIPVDKRLYAGGAGSVRGYAYQR
                     AGPLGPGDVPLGGISSIELGLELRYRITDSIGIAPFVEGGNVYPHSLPDSLELFYGAG
                     IGLRYYTLIGPIRLDLATPFTRRPGDDPIQVYISIGQAF"
     gene            2543..3214
                     /locus_tag="VGP50_07390"
     CDS             2543..3214
                     /locus_tag="VGP50_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027238.1"
                     /translation="MRLTGLLVSIATLLAPGVAVAQDAAGARSGFTLGPTISTLGAGA
                     DAGFRAGPYLGVRLDASFLSFDYNRSIDSIPYKFGVNLRSGGPVLDLYPFAGSFHLTA
                     GARINGNGADVSATPGSNVTINGDTFTPAQVGTLTGSLKYNRVAPYLGIGYSGRPTRW
                     LELGLDLGVLYQGKPSVNLAASGQFANNPSLAADIAQEETNVENRLGFTTWYPVVTFA
                     ALFHF"
BASE COUNT          556 a         1092 c         1158 g          558 t
ORIGIN      
        1 cgtcaacgtc gcgggctcgc ccaggaggtc gcgcccgagc tcgatgcgat ccaactgcag
       61 ccgctcgatc gtcacggcga gaggcgggtg gagaagcgcc gcaaggtcgc tgccgccgct
      121 tgcactctga gggccgcgct cgatgcgcag cgcgcgcgcc gcgatccgca ccagtgtgag
      181 ccgtccgacg aggagatcgg cgggcgccac ggcgagggcg gtatcgctga ggacgatcct
      241 cggcccttgg gaatccgcga cggtgatcgc gtcgaccgtc atatcgaacg gcacgaatcc
      301 gccgatatga tcaatgctca cacgctccgc ggggtccgag agccagcgct cgatcagcga
      361 tgcgagcgcc gccttgcccg gtctggtctg cgccaagccg aagacaagcg cgaccacgag
      421 catcagcccg agaaggccga tgccgatgcc ggccagcgct ttgcgcaacg tcctcatgtc
      481 gcggaaacca tcagaatgcc tgtccgatac tgatatagac ctgaatcggg tcgtcgcccg
      541 gccggcgtgt gaagggtgtt gccaggtcga ggcggattgg gccgatcagc gtgtagtagc
      601 gcagcccgat gccggcgccg tagaagagct ccaggctgtc gggcaggctg tgcgggtaga
      661 cgttgccgcc ttcgacgaac ggggcgatgc cgatgctgtc ggtgatccgg tagcgcagct
      721 cgaggccgag ctcgatgctg gagatgccgc ccagcggcac atcgcccggg cccaaagggc
      781 cggcgcgctg ataggcgtaa ccgcgcaccg atcccgcccc gccggcgtag agtcgcttgt
      841 cgacggggat ttcgtcgcgc gtcgcgccga aaatcgagcc aaaggcggcg aagcttgcga
      901 gcacgagccg cccgctgtcg ttgagccgct cgtagtcgcg ctcctcgatg cgcgagttga
      961 cgaaattgag gttgggtccc gaaaggctgt gataggggat gagcgtcagc gtctggcgcg
     1021 agccgagggt gggatcgagg agatcgtcgg tggtgtcgcg gcgcagcagg aacggcagcc
     1081 cgagcaacgt gtagtcctcg gtcccgaaga cgtccgactg cacattggca tgctcgaagg
     1141 aaacgccgcc atcgacggtg atcgacggca gcagcggacg ctcgagtccg aggaaggcct
     1201 gctcgcgcaa gctgcgatag gcatcggtgt tctgctggag cagtgcggcg ttcgccagaa
     1261 gctcctggtt gcgatcgaga aaatcgggct tgcggaaggc gagcgccaat ccgtcctgac
     1321 gctgcgcgac ggcggcggtg atgcgcagat tctccgcgcc gccgaagaga ttgcgatgct
     1381 cccagaaaat ctggccgccc gcgccgagat tggtgttgta ggcgacgccc gcgccgaccg
     1441 agcgcggtgg gccctcgacc agatcgaccg tcatcgccac ctgcccggcc ccgtccggcg
     1501 catcgctatg attgatgcga gcggcgctga acaggccgct cttgacgagg ccctggcgcg
     1561 tcttctccac caggcgcgcg tcgtagagcg cgccctcgcg ccaggcgatc cgcgcttcga
     1621 cataggcgcg gtcgacatgt tcgaggccgc tgatggtgac agggccaaaa tgtgcctccg
     1681 gtccggggtc gatggtgtag gtgacgctca tcgtatgctt ggccacgtcg acgacggcac
     1741 gatgctcgac gaccttggcg aaaggatggc cgttgcgtgc gtaggtctcg acgatctgct
     1801 gctcggccgc tcccaccggg gccgaccgcg ccggttcgcc gatggttaac ccgagagcgg
     1861 cggagccgag ctgatcaaga agcggcagcg cctcccccga cgtgccgtga aaggtagcgc
     1921 tttcgagatg gaagagtggc ccgggatcga cgatgacctc gaccgtcacc gagtcgtctt
     1981 tgggctcgag ccggtaggag agctgcggtg tccaatagcc ggcggcctgc accacctcct
     2041 tcagtcgcgg cagatcgtcc tcggcgcgcc ggcggagcgc cgcctcggag ggcggcggct
     2101 tgtcctcgag cttcaccagc tgcgatacgt cccgcagagt gcgctccagc gccgaatctt
     2161 tcaccccctc gaggtgaatc tcgctgctgt aggagatgtc gctggtggca ggggaggcgt
     2221 gccagaggac gaggaacgca gccaccagga tggccagcgg ccatagaggt cgcgcggcga
     2281 agggatggtc acgctgggga gaggtcaccg ggtggccgga ggtgaagcgg gatgtgcagg
     2341 gatcggacgg gcgatcttaa ccgctccgcg cgcggacaca cacaaaagtg acagggctgt
     2401 tgggttgaac agttcaggga aacaggtatc gtcgctacct atgtggttga ttcgccgctg
     2461 gagaccccgc gccggcatcg gccgctcggc ccggtcggtc gtcgcgatct tgctggcggt
     2521 ggccgggtcg ctgacttctt ccatgcgctt gacgggcctc ctggtatcga tcgcgacgct
     2581 cttggcgccg ggggttgcgg ttgcgcagga cgcggccggc gcgcgctcgg gcttcacgct
     2641 cgggcccacc atcagcacgc tcggcgccgg cgccgatgcc ggctttcgcg ccggtcccta
     2701 tctcggcgtc aggctcgatg cgagcttcct cagcttcgac tacaaccgca gcatcgacag
     2761 cattccctat aaattcgggg tcaatctacg ctcgggcggg cccgtgctcg atctctatcc
     2821 cttcgccggc agctttcatc tgactgcggg cgcgcgcatc aacggcaacg gcgccgatgt
     2881 cagcgcgacg cccgggtcga atgtgaccat caatggcgac accttcacgc ccgcccaggt
     2941 cggcactctg accggctcgc tcaaatacaa ccgcgtcgcg ccctatctcg gcatcggcta
     3001 ctcgggccgc ccgacgcgct ggctcgagct cggcctcgac ttgggcgttc tctatcaggg
     3061 caagccgagc gtcaacctcg ctgccagcgg ccagttcgcg aacaatccct cgcttgccgc
     3121 cgatatcgcc caggaggaga cgaatgtcga gaaccggctc ggcttcacga cctggtaccc
     3181 ggtcgtcacc ttcgccgcgc tgttccactt ctgacgcgag ccgatctcgc gccgtttgac
     3241 tccctcgccg cggcgctgcc aatctggtcg gcaaagaagg accgacccgg gggaggcaga
     3301 tcatgagaac gctcgtcgcg gcgcttgccg cgctcgcctt gctggctttc ggcgagagcc
     3361 gtgc
//
LOCUS       DASYNS010000236        11101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_522368, whole genome shotgun sequence.
ACCESSION   DASYNS010000236 DASYNS010000000
VERSION     DASYNS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11101
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522368"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VGP50_07395"
     CDS             <1..1044
                     /locus_tag="VGP50_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV8027239.1"
                     /translation="TVPGITDLAVLASLGQPTIKIDIDRARAARYGLMPGDINATVAA
                     AIGGQAAGDLFEDGSDRHFPMIIRLAAEYRQSLDAIRNIAIGAQNPSGNGVVQIPLSE
                     VAKVSLVSGASFIYREQQERYIPIKFSVRDRDLGGAVIEAQQKVAEQVQLPGGYRLEW
                     VGEFGNLEEAVARLLLVVPLSIALIGILLYVNFGSLVDTLLAASVIPMALIGGIFALY
                     VTGTPFSVSAAIGFVALFGIAAMDGIIVLSYYNSVISTGVDRVTAITRACEVQMRPVM
                     MTCIVACVGLLPAALSTGIGSQVQKPLALVVVGGILLAPFLILLVLPVLVELFSQRQA
                     AAPIDAAQPEPAE"
     gene            1250..2722
                     /locus_tag="VGP50_07400"
     CDS             1250..2722
                     /locus_tag="VGP50_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEV8027240.1"
                     /translation="MRGRAGWGLLALALLSACAVGPDFQRPAPPSAQSYTPEPLSAET
                     SAAAVRGGAAQRFVPGGDLPAEWWTLFHSAPLNALIAQALAGNPDLAAGQAALRAAFE
                     NVYAQEGFFFPSVDANFSPSRQKNPTAVLAPTLASGTPIFNLYTAQVSVSYTPDVFGL
                     NQRTVESLAAQADTQRFALEATYLTLTSNVVAGAIQEASLRAQIQATQEIIDIERQLL
                     DLLRRQNALGQIAEADVVAQEATLAQLEQSLPPLQKQLAQQRDLLTALAGRLPSEEVS
                     ERFELASLELPEELPLSLPSVLVEQRPDIRAAEANLHAASAQVGVAIANRLPNVTLTA
                     ADGTTAAQLDRLFTSGTGFWSVAGSLTQPIFAGGTLLHRQRAAEAAYDETAAQYRSAV
                     VTAFRNVADTLRALQSDADGLGAAVRAESAASRSLEIARRQLQLGQTSYLSLLNAEQT
                     YQQARIALVQAQANRYADTAALFQALGGGWWHRTEAKSGG"
     gene            2689..3195
                     /locus_tag="VGP50_07405"
     CDS             2689..3195
                     /locus_tag="VGP50_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8027241.1"
                     /translation="MAPHRGKERRLTPMSITVERVMAVTSEVRALVEALDQALAALYV
                     PEQCHGLSLDRLFEPNLRFFVARIEGESAGCGGVALFSDYAEVKRMYTKAALRGRGVA
                     KAVLRRLEAEARSAGVTVLRLETGKHQPEAIGLYEESGFRRRSAFGPYAEMPASAVAL
                     SLFYEKPL"
     gene            complement(3202..4563)
                     /locus_tag="VGP50_07410"
     CDS             complement(3202..4563)
                     /locus_tag="VGP50_07410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV8027242.1"
                     /translation="MTTPTKTALRPAASAGVLALCLLAGCSTAMQGSTSDPDFALYRQ
                     VIERVQGSYVEPVNEEELVKASLKGMLNGLDPHSDYMDENEYSEMLADSHGEFAGIGT
                     ELTRDENHPKIISPIEDTPAARAGLKPGDVILRIDGHPTEGMSLKEAVDELRGPAGSK
                     VKIGILRTGQRPFDVTLTRAVIHVASVKAHLESGRIGYIRVTTFADKTQTEFLDALDG
                     LEHGAGGKLNGMVLDLRNDPGGRLDTAVEVAGDFLDGGTVVSTRGRGLGEDKDYGAPA
                     AGDQLRGVPVVVLVNGASASASEIVAGALQDRHRASVIGTRTFGKGSVQTIIPLDGRG
                     ALRLTTARYYTPEGRSIQGEGITPDVIVLPPRDQQIAQATVLHETDLRGALKNTGPDH
                     GSGPEQNHAAAGVSDTPTPDEDSETAAIDPTIIGTGKDYQLVVALKRLKEMVARNAPA
                     GRS"
     gene            4753..6300
                     /locus_tag="VGP50_07415"
     CDS             4753..6300
                     /locus_tag="VGP50_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV8027243.1"
                     /translation="MSWEAEIEELRRREALARQMGGADKVKRQHEGGKLTVRERIDAL
                     LDPGSFHEIGAIAGRAQYRPDGTLEHFMPANFVVGRGRIDGRPVVVGGDDFTVRGGAA
                     DAAIRGKQVMAEQLAQELRLPLVRLVDGTGGGGSVKTLEVEGRTYVPANPAWDLVVAN
                     MGTVPVVALALGSVAGLGAARLVTSHYSLMVEGSSQMFIAGPPVVARLGENLTKEELG
                     GSEIHARSGAVDDVVASEPEAFLRARRFLSYLPSSVFELPARGPVADAPERRDAWLIE
                     AVPRDRRKVYDMRRILGAVLDRDSFFEIGRKFGGSAITGLARLDGWPVAVLASDPFIY
                     GGGWTAQASQKVTRFVDLANTFHLPVVHFVDIPGFVIGLDAEKAGTIRHGARALAAIY
                     QATVPWCSILVRKAFGVAGAAHQNHARLHYRYAWPSGDWGSLPVEGGIEAAYRAELEA
                     SPDPAKLRGEIEERLNRYRSPFRVAEAFGVEEIIDPRDTRPLLCEFAGLAAGLRAPGP
                     VTTAMRP"
     gene            complement(6568..7830)
                     /locus_tag="VGP50_07420"
     CDS             complement(6568..7830)
                     /locus_tag="VGP50_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027244.1"
                     /translation="MTVTRRSRQLSRRSVLGLGAAASLLPAAAWAETENLRVGFLTVN
                     TGPLAAGGKQMEEGLTLFLKDRNGMIAGRKVELITGDTAGLPATAKSKTQELVERNNV
                     QVLIGPLATYEAMAIDDYIRQGQVPLITPTSAASVGATGVEVNPWLIHAVGTAPQVTH
                     PLGEYAAKTLNYKRVATIADDFTYGYEGVGGFQRTFEDNGGKIVQKIWAPLNATDYGT
                     FIAQIKPDVDAVYIGFAGVNGLRFLKQYADYGLHGKIPVLGNATTTDEGILRVMGDEA
                     LDVVTASWYSATLDTPANRGFVDGIRVNFGHDPGFYSVGTYTAGLFLETALTALQGDT
                     SDPEKLRKTLAASKLAYGPIGAIRLDNFGTPVLDVHIRKVEKRSGKLVNTVINTYHDV
                     SQFWTYDPQKFLADPAYSRAFPAGKYLE"
     gene            7983..8606
                     /gene="pncA"
                     /locus_tag="VGP50_07425"
     CDS             7983..8606
                     /gene="pncA"
                     /locus_tag="VGP50_07425"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEV8027245.1"
                     /translation="MNRDDSDVLLVIDIQNDFCPGGALAVVEGADIVPVANRLGERFP
                     HVVMTQDWHPPGHQSFASAHPGRKPFETITLAYGPQTLWPDHCVQATPGAALHPGLRL
                     PRAELVIRKGFRPAIDSYSAFFENDRRTPTGLAGYLRERGLERLFLVGLATDFCVHYS
                     AIDARRLGFAVVLVEDGCRAIDLDGSLDLAMRAMAAAGVARVTSAAL"
     gene            8754..9191
                     /locus_tag="VGP50_07430"
     CDS             8754..9191
                     /locus_tag="VGP50_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HEV8027246.1"
                     /translation="MQQPTQINDFLSVAGQISKDDIAAIAQAGFAVIINNRPDNEEPG
                     QLDHHEAEAEAKKRGLEYRYLPVTTGNITRTEVAEFARLLLRSPKPVLAHCRSGTRCY
                     LLWAVGRVLFEHQSALTLVAEAAAKGYDLRILPQLVEKLEAPG"
     gene            complement(9355..10182)
                     /locus_tag="VGP50_07435"
     CDS             complement(9355..10182)
                     /locus_tag="VGP50_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV8027247.1"
                     /translation="MQYVEVKGTRVPAIGLGTWQLSGRGCYESVREALELGYRHIDTA
                     QMYGNEHEVGRAVRDSGVARDAVFITTKLAPGNLAPAAVQRSTEESLERLGMDHVDLL
                     LIHWPSSSVKLGETLHAMAALHAAGKARHLGVSNFTVKLLAEAAATGAAELLCNQVEY
                     HPFLSQRPVLAALATHGMMLTAYSPLARGRVAREATLLAIARKYGKSPAQVTLRWLID
                     QERVAAVPKAAGREHLAANLDIFDFALSAEDRAAIGALRGSERVVDVAGWSPRWDPP"
     gene            10268..>11101
                     /locus_tag="VGP50_07440"
     CDS             10268..>11101
                     /locus_tag="VGP50_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEV8027248.1"
                     /translation="MRILVLGAGAIGGYYGGRLVEGGADVTFLVRPGRARQLAERGLV
                     IRSALGDVERPVKTVLAEAVREPFDLVLLSAKAYDLDSAIAAIAPALGPHSAVLPLLN
                     GINHLAALSGRFGTARVLGGCCGIGASLEASGEVRHVGTSEWLTFGEQSGERSARCDT
                     IAQIFATTKVNATLSDNILQAMWDKFVMIASLAAATTLTRANVGEILAAPSGEGLMLE
                     SLAECERVAAADGHAPTAPSLERTRKMLTTRGSPFTASMMRDLVAGRPTEGDHIIGDL
                     VR"
BASE COUNT         1661 a         3788 c         3774 g         1878 t
ORIGIN      
        1 acggtgccgg ggatcaccga cctcgccgtg ctggcctcgc tggggcagcc caccatcaag
       61 atcgacatcg accgggcgag ggcggcgcgc tatggcctga tgcccggcga catcaacgcg
      121 accgtcgccg cggcgatcgg cgggcaggcg gcgggcgacc tcttcgagga cggcagcgac
      181 cgccatttcc cgatgatcat ccgcttggcg gccgagtatc gccagagcct cgatgccatt
      241 cgcaacatcg ccatcggcgc gcagaacccg agcggcaacg gcgtggtgca gatcccgctc
      301 agcgaggttg ccaaggtgag tctcgtctcg ggcgcttctt tcatctaccg cgagcagcag
      361 gagcgctaca tccccatcaa gttcagcgtg cgcgaccgcg atctcggcgg cgccgtgatc
      421 gaggcgcagc aaaaggtagc cgagcaggtg cagctgcccg gcggttatcg cctcgaatgg
      481 gtcggcgagt tcggcaacct cgaagaggca gtggcgcggc tcctcctggt ggtgccgctc
      541 agcatcgccc tgatcggcat cctgctctat gtcaacttcg gttctctggt ggatacgctg
      601 cttgccgcca gcgtcattcc gatggcgctc atcggcggca tcttcgcgct ctatgtcacg
      661 ggcacgccgt tcagcgtctc cgcggccatt ggctttgtcg ctctcttcgg catcgccgcc
      721 atggacggca tcatcgtgct ctcctactac aacagcgtca tcagcaccgg tgtcgaccgt
      781 gtcaccgcca tcacgcgcgc ctgcgaagtc cagatgcggc cggtcatgat gacctgcatc
      841 gtcgcttgcg tcggacttct gccggcggcg ctctccaccg gcatcggctc gcaagtgcag
      901 aagccgctgg cgctcgtggt ggtgggtggg atcctgctgg cgccgttcct cattctcctc
      961 gtgctgccgg tgctggtcga gctgttctcg cagcggcaag cggccgctcc gatcgacgcg
     1021 gcgcagcccg agcccgcgga gtaggccaat ggccggagga ctgcatcggg aatgttcccc
     1081 tctcccgttg aggctatccc gtattgggtt caccacggag gcacggagga cacggagaag
     1141 aggggttcgg cgcgcgacgc gcgccatcct tcgcttctcc gtgctctctg tgtctccgtg
     1201 gtcaatcttt cctatcggcg tctcggcggc atgtgcgatt gccctgccct tgagggggag
     1261 ggcagggtgg gggctcctgg cgctggcgct tttgtcggcc tgcgcggtcg ggccggattt
     1321 tcagcggccg gcgccgccgt cggctcagtc ttacacgccc gagccgctca gcgccgagac
     1381 cagcgcggcc gcggtccgcg gcggcgcggc gcagcgcttc gtgccgggcg gcgatcttcc
     1441 ggccgaatgg tggacgctct tccattcggc gccgctcaac gccctcatcg cgcaggccct
     1501 cgccggcaat ccggacctcg ccgcagggca ggcggcgttg cgcgccgcct tcgagaatgt
     1561 ctatgcgcaa gagggtttct tttttcccag cgtcgacgcg aacttctcgc ccagccgcca
     1621 gaagaacccg accgcggtat tggcgccgac cctcgcttcc gggacgccga tcttcaacct
     1681 ctacaccgct caggtgagcg tgtcctacac gcccgatgtc ttcgggctca accagcggac
     1741 ggtcgaatcg ctggcggcac aggccgacac gcagcgcttc gcgctcgaag ccacctatct
     1801 gacattgacc tccaacgtcg tcgccggcgc gattcaagaa gcgtccctgc gcgcccagat
     1861 tcaggcgacg caggagatca tcgatatcga gcggcagctc ctcgatctgc tgcgacgcca
     1921 gaacgccctg gggcagatcg ccgaggccga tgtcgttgcg caggaagcga cgctggcgca
     1981 gctcgagcaa agcctgccgc cgctgcaaaa gcagctggcg cagcagcgcg atctgctgac
     2041 ggcgctcgcc ggccgcctgc cgagcgagga ggtgagcgag cgcttcgagc tcgccagcct
     2101 cgagctgccc gaggagctgc cgctcagcct gccatcggtg ttggtcgagc agcggccgga
     2161 tatccgtgcc gccgaagcca atctccatgc ggcgagtgcg caggtcggcg tggcgatcgc
     2221 caaccggctg ccgaacgtca cattgaccgc cgcggacggc acgacggcgg cgcagctcga
     2281 tcgcctgttc acgtcgggaa ccggcttctg gagcgtggcc gggagcctga cgcagccgat
     2341 cttcgccggc ggcacgctgc tgcaccgcca gcgcgccgcc gaggccgctt atgacgagac
     2401 ggccgcgcaa tatcgcagcg ccgtcgtcac cgccttccgc aatgtcgccg acacgctgcg
     2461 ggcgctccag tccgatgccg acggcctcgg cgccgcggtg cgcgccgaga gcgcggcgtc
     2521 acgcagcctc gagatcgccc gccgccagct ccagctcggc cagaccagct atctctcgct
     2581 gctcaatgcg gagcagacct atcagcaggc gcgcatcgcg ctcgtgcagg cgcaggcgaa
     2641 ccgctacgcc gacacggcgg ccctcttcca ggcgctgggc ggcggctggt ggcaccgcac
     2701 cgaggcaaag agcggcggct gacgccgatg agcatcacgg tcgagcgcgt catggcggtc
     2761 acttcggagg tccgagcgct cgtcgaggca ttggaccagg cgctcgcggc gctctacgtg
     2821 ccggagcaat gtcacggcct ttcgctcgat cgtctgttcg agccgaatct gcgcttcttc
     2881 gtcgcgcgga tcgagggcga gtccgcgggg tgcggcggcg tcgcgctctt cagcgactat
     2941 gcggaagtga agcgcatgta caccaaggcg gcgctgcgcg gccgcggtgt cgctaaggcg
     3001 gtcttgcgcc gcctcgaagc ggaagcgcgc tcagccggag tgacagtgct gcgtctcgag
     3061 accggcaagc atcagcccga agccatcggc ctttacgagg aaagcggctt ccgtcggcgt
     3121 agcgccttcg gtccctatgc cgaaatgccc gcctcggcgg tcgcgctcag cctcttctac
     3181 gagaagccgc tataggcccg gtcagcttct tccggcggga gcgttgcggg cgaccatttc
     3241 cttgagccgc ttcagcgcaa ccaccagctg gtagtccttg cccgtgccga tgatggtcgg
     3301 atcgatcgct gcggtctcgc tgtcttcgtc gggcgttggc gtatcggaga cgccagcggc
     3361 agcgtggttc tgctcggggc cgctgccgtg gtcggggccg gtgttcttga gggcgccgcg
     3421 caggtcggtc tcgtgcagca cggtcgcctg ggcgatctgc tgatcgcgcg gcggcaggac
     3481 gatgacgtcg ggcgtgatgc cttcgccctg gatcgagcgt ccctcgggcg tgtagtagcg
     3541 cgccgtggtc aggcgcaacg ctcctctgcc gtcgagcgga atgatcgtct gcaccgatcc
     3601 cttgccgaag gttcgggtgc cgatgacgct cgcgcggtgg cgatcctgca gcgctcccgc
     3661 cacgatctcc gacgccgacg ccgacgcacc gttcacgagc accaccaccg gcacgccgcg
     3721 cagctggtcg ccggcggcgg gtgcgccata gtccttgtct tcgccgagac cgcggccgcg
     3781 cgtcgagacg accgtgccgc catcgagaaa gtcgccggcg acctcgaccg ccgtgtcgag
     3841 acggccgccc ggatcgttgc gcagatcgag caccatgccg ttgagcttgc cgccggcccc
     3901 atgctcgagg ccgtcgagcg cgtcgaggaa ctcggtctgc gtcttgtcgg cgaaggtggt
     3961 gacgcgaata tagccgatgc gtcccgattc gagatgcgcc ttgaccgagg cgacatggat
     4021 caccgcccgc gtcagcgtca cgtcgaaggg acgttgtccc gtgcgcagga tgccgatctt
     4081 gaccttcgag ccggccgggc cgcgcaactc gtcgacggct tctttgaggc tcatcccctc
     4141 ggtcggatgg ccgtcgatgc gcaggatgac gtcgccgggc ttgaggccgg cgcgggccgc
     4201 cggggtgtcc tcgatcggcg agatgatttt cgggtggttc tcgtcgcgcg tcagctcggt
     4261 gccgatcccg gcgaactcgc catggctgtc ggcaagcatc tcgctgtatt cgttctcgtc
     4321 catatagtcc gaatgaggat cgagcccgtt gagcatcccc ttgaggctcg ccttgacgag
     4381 ttcctcctcg ttgaccggct cgacgtagct tccctggacg cgctcgatca cctgacgata
     4441 gagcgcgaag tcggggtcgc tggtgctgcc ctgcatggcc gtgctgcagc cggcgagcag
     4501 gcagagcgcg aggaccccgg ccgaggcggc ggggcgtaga gcggtcttcg tcggggtggt
     4561 cacgatcctt gcaccttgaa atggccgggt cattctctca aaaggcgaat gcatgcctgc
     4621 gtacgtgccg gagagccgcc accgtccagc tatatagaga acgctccggc tgccgtcatc
     4681 ctgacaaata cgcaacttta tcgacaggca tcgctcgtta tggtgaagat gcggggcgaa
     4741 tgaggaacga ggatgagttg ggaagcggaa atcgaggagc tgcgccggcg tgaggcgttg
     4801 gcgcggcaaa tgggcggtgc cgacaaggtc aagcgccagc acgagggcgg caagctcacg
     4861 gtgcgcgagc gcatcgacgc gctgctcgat cccggcagct tccacgagat cggcgccatc
     4921 gccggccgcg cgcaataccg gccggacggc acgctcgagc acttcatgcc ggcgaatttc
     4981 gtcgtcggcc gcggccgcat cgacggccgc ccggtggtcg tgggcggcga cgatttcacg
     5041 gtgcgcggcg gcgctgccga cgccgcgatc cgcggcaagc aggtcatggc cgagcagctc
     5101 gcgcaggagc tgcgcctgcc gctcgtccgt ctcgtcgacg gcaccggcgg cggcggctcg
     5161 gtcaagacgc tcgaggtgga gggccggacc tatgtgccgg ccaaccccgc ctgggatctc
     5221 gtcgtcgcca atatgggcac cgtgccggtg gtggcgctcg cgctcggctc cgtggcgggc
     5281 ctgggcgccg cccggcttgt cacgagccat tattcgttga tggtcgaagg cagctcgcag
     5341 atgttcatcg ccgggccgcc ggtggtcgcg cgtctcggcg agaatctcac caaggaggag
     5401 ctgggcggca gcgagatcca cgcccgctcc ggcgccgtgg acgacgtcgt cgcgagcgag
     5461 ccggaagcct tcctccgcgc gcgccgcttc ctctcctatc tgccgtcctc ggtgttcgag
     5521 ctgccggcgc gcggcccggt cgccgacgcg cccgagcggc gcgacgcgtg gctcatcgag
     5581 gcggtgccgc gcgaccgccg caaggtctac gacatgcgcc gcatcctcgg cgccgtgctc
     5641 gaccgcgaca gctttttcga gatcggccgc aaattcggcg gctcggcgat caccggcctg
     5701 gcgcggctcg atggctggcc cgtggccgtg ctggcgagcg atcccttcat ttatggcggc
     5761 ggctggacgg cgcaggcgtc gcagaaggtg acgcgcttcg tcgacctcgc caacaccttc
     5821 catctgccgg tcgtgcattt cgtcgatatt ccgggcttcg tcatcggcct cgacgccgag
     5881 aaggcgggca cgatccgcca cggcgcccgc gcgctcgccg ccatctatca ggccacggtc
     5941 ccctggtgct cgatcctggt gcgcaaggcg ttcggcgtcg ccggtgcggc gcaccagaat
     6001 cacgcgcggc tccattatcg ctatgcctgg ccttccggcg actggggctc gctgccggtc
     6061 gagggcggta tcgaggccgc ttatcgcgcc gagctcgagg cgtcgcccga tcccgcgaag
     6121 ctccgcggcg agatcgagga gcgcttgaac cgctaccgct cgccctttcg cgtcgccgaa
     6181 gcattcggcg tcgaggagat catcgatccg cgcgacacgc ggccgctgct ctgcgagttc
     6241 gccggcctcg ccgccggcct gcgcgcgccc ggccccgtca cgaccgcgat gcggccgtag
     6301 cgacggcgta ctcgcgacaa aaacgaaccc tctcccctcg cggggcaggg caatcgcata
     6361 tgccgccgac acgccgatag gaaagatcga ccacggcggc acggagaagc gaagaatggc
     6421 gcgcctcgcg cgccgaaccc ctcttctccg tgttctctgt gcctccgtgg tgaaaccgat
     6481 atgcgatagc cctggcgtga ggcgggccgt cgccgcgccc gcgcgtttcc gatggcgctc
     6541 cgcgtcgccg ctaccgcgcg cagcccgcta ttcgagatat ttccctgccg gaaaggcgcg
     6601 cgaataggcg gggtcggcga ggaatttctg gggatcgtag gtccagaact ggctgacgtc
     6661 gtgataggtg ttgatgaccg tgttgaccag cttgccgctg cgcttctcga ccttgcggat
     6721 atgcacgtcg aggacgggcg tcccgaaatt gtcgaggcgg atcgcgccga tcggcccgta
     6781 ggcgagcttg ctcgcggcga gggtcttgcg cagcttctcg ggatcggagg tatcgccctg
     6841 gagcgcggtc agcgccgtct cgagaaacag cccggccgtg taggtgccga cggaatagaa
     6901 gcccggatca tgaccgaaat tgacccggat gccgtcgacg aagccgcgat tggccggcgt
     6961 gtcgagggtc gccgaatacc agctggcggt cacgacgtcg agcgcttcgt ctcccatcac
     7021 gcgcaggatg ccttcatccg tggtggtggc gttgccgagc acgggtatct tcccgtgcaa
     7081 tccgtaatcc gcgtactgct tgagaaagcg caagccgttg acgccggcga agccgatata
     7141 gaccgcgtcc acgtcaggtt tgatctgcgc gatgaaagtg ccgtagtcgg tggcgttgag
     7201 cggcgcccag atcttctgca cgatcttgcc gccattgtcc tcgaaggtcc gctgaaagcc
     7261 gcccacgcct tcgtagccat aggtgaaatc gtcggcgatg gtggcgacac gcttgtagtt
     7321 gagggtcttc gccgcatatt cgccgagcgg atgggtgacc tgcggcgccg tgcccacggc
     7381 gtggatcagc cagggattga cctccacgcc cgtggcgccc accgaggcgg ccgaggtcgg
     7441 cgtgatgagc gggacttgcc cctggcggat atagtcgtcg atcgccatcg cttcatacgt
     7501 cgcgagcggc ccgatcagca cctgcacatt gttccgctcg acgagctcct gcgtcttgct
     7561 cttggcggtg gcgggcagcc cggcggtatc gcccgtgatc agctcgacct tgcggcccgc
     7621 gatcatgccg ttgcggtcct tgagaaagag cgtgaggcct tcttccatct gcttgccgcc
     7681 ggcggcgagc gggccggtgt tgacggtgag aaagccgacg cgcaaattct cggtctcggc
     7741 ccaggcggcg gcaggcaaaa ggctcgcggc ggcgcccaac cccagcacgc tccggcgtga
     7801 cagctggcgc gagcgtcgag taacggtcat ggcgtcattc ccccttggca gcggcgctgg
     7861 cgcgcggcgc ggacaagctg cgattgtggc gccgggaatg caagcgccct gttggcgcat
     7921 ggcccctgca atcgcttggc ttttgtgcga aatgccggcg aacgctgcca aggaggcggc
     7981 tcatgaacag agacgatagc gacgtcctcc tcgtcatcga tatccagaac gacttctgcc
     8041 cgggcggcgc gctggccgtg gtcgagggcg ccgacatcgt gccggtcgcc aaccgcttgg
     8101 gcgagcgctt tccgcatgtg gtgatgacac aggactggca cccgccggga caccagtctt
     8161 tcgccagcgc gcatccgggg cgtaagccct tcgagacaat cacgctcgct tacggcccgc
     8221 agacgctctg gcccgatcat tgcgtgcagg cgacgccggg cgccgcgctt catccgggtc
     8281 tccgcttgcc gcgggcggag ctggtgatcc gcaagggctt tcgtccggca atcgattcct
     8341 attcggcctt tttcgagaat gaccgccgca cgccgaccgg gctcgccgga tatctccgcg
     8401 agcgcggcct cgaacgcctc ttcttggtcg gcctcgccac cgatttctgc gtccactatt
     8461 cggcgatcga cgcgcggcgt ctcggcttcg ccgtcgtgct ggtcgaggat ggctgccgcg
     8521 ccatcgatct cgacggctcg ctcgatctag ccatgcgcgc catggccgcg gccggcgtcg
     8581 cgcgcgtaac cagcgccgct ctctagtccc tctcccgcac cgcgggagag ggagggaccc
     8641 actgcgaagc ttcagcgaag cagtgggagg gtgaggggac ctcgcaaacc tcgcccggtc
     8701 gccgggcgac ggccgccgtg ctatgaatgg cgcagcgcca agggaggcga gagatgcagc
     8761 agccgacgca gatcaacgat ttcctgagcg ttgccgggca gatcagcaag gacgacattg
     8821 cggcgatcgc ccaggcgggc ttcgccgtca tcatcaacaa ccggcccgac aacgaggagc
     8881 cgggacagct cgaccaccac gaggccgagg ccgaggccaa gaagcgcggt ctcgaatatc
     8941 gctatctgcc cgtgacgacc ggcaacatca cgcgcacgga ggttgcggag ttcgcgcggc
     9001 tgctgctgcg cagcccgaag ccggtgctcg cccattgccg gagcggcacg cgctgctatc
     9061 tcctgtgggc ggtgggccgc gttctcttcg agcatcagag cgctttgacg ctcgtcgccg
     9121 aggcagcggc caaaggctac gatctccgca tcctgccgca gctggtcgag aagctcgaag
     9181 cgccaggctg accggggcga gacaacatct cgatcacgtg cgatcggcac ttcctaactt
     9241 cgtcatcccc gcgaaagcgg gggcccaggg cgagcgacgt gccggcggcc cctggatccc
     9301 cgctttcgcg gggatgacga cgaaggaacg gtttcccgta aacgggatgc gttcttaagg
     9361 cgggtcccag cgcggcgacc agccggcgac atcgacgacg cgctcgctgc cgcgcagcgc
     9421 gccgattgcg gcgcggtcct cggcgctgag cgcgaaatcg aagatgtcga gattggccgc
     9481 gagatgctcg cggccggccg ccttggggac ggccgcgacg cgctcttggt cgatgagcca
     9541 gcgcagcgtc acctgcgccg gcgacttgcc gtatttgcgc gcgatggcga ggagcgtcgc
     9601 ttcgcgcgcg acgcgcccac gcgccagcgg cgagtaggcg gtgagcatca tgccgtgggt
     9661 cgcaagcgcc gccagcaccg gccgctgcga caggaaggga tgatattcga cctggttgca
     9721 caggagctcg gccgcgccgg tcgccgccgc ctccgcgagc agctttacgg tgaaattgct
     9781 gacgcccaga tgccgggctt ttccggccgc atgcaacgct gccatcgcat ggagtgtctc
     9841 gccgagcttc acgctgctgc tcggccaatg gatcaacagc aggtcgacat ggtccatgcc
     9901 gaggcgctcc aggctctcct cggtcgagcg ctgcacggcg gcgggcgcga gatttcccgg
     9961 cgcgagcttg gtggtgatga agaccgcgtc gcgcgcaacg cccgaatcgc ggacggcgcg
    10021 cccgacctcg tgctcgttgc cgtacatctg cgccgtgtcg atgtggcggt agccgagctc
    10081 gagcgcttcg cgcacgctct catagcagcc gcgtcccgag agctgccagg tgccgaggcc
    10141 gatcgccggc acgcgcgttc ccttgacctc gacatactgc atccctgtct ctccctttga
    10201 ggggtggcgc cgcccgtcgc cggacggcta gcatgccggc agcgaaaacg ccaatgcgga
    10261 ggacggcatg cggattctgg tgctcggtgc cggcgccatc ggcggctact acggcggccg
    10321 tctcgttgaa ggcggcgccg acgtgacctt cctggtgcgg cccggccgcg cgcgccagct
    10381 cgccgagcgc gggctcgtga tccgcagcgc cttgggcgat gtcgagcgcc cggtcaagac
    10441 cgtgctcgcc gaggcggtcc gcgagccctt cgacctcgtg ctgctctccg cgaaggccta
    10501 tgacctcgac agcgccattg ccgcgatcgc gccggcgctc ggcccgcaca gcgcggtgct
    10561 gccgcttctc aacggcatca accatctggc ggcgctcagc ggccgtttcg gaacggcgcg
    10621 cgtgctcggc ggctgctgcg gcatcggcgc gtcactcgag gcctcgggcg aagtgcggca
    10681 tgtcggcact tccgaatggc tcaccttcgg cgagcaatcg ggcgagcgct cggcgcgctg
    10741 cgacaccatc gcccagatct tcgccacgac caaagtcaac gcgacgctga gcgacaacat
    10801 tctccaggcg atgtgggaca agtttgtcat gatcgcttcg ctcgccgccg cgacgacgct
    10861 gacgcgcgcc aatgtcggcg agatcctggc ggcgcccagc ggcgaagggc tgatgctgga
    10921 gtcgctcgcc gagtgcgagc gcgtcgccgc cgctgacggc cacgcgccga cggcgccctc
    10981 cctcgagcgc acgcgcaaga tgctgacaac ccgcggttcg cccttcaccg cctcgatgat
    11041 gcgcgatctc gtcgccgggc gccccaccga gggcgaccac atcatcggcg acctggtgcg
    11101 c
//
LOCUS       DASYNS010000237         2949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2083064, whole genome shotgun sequence.
ACCESSION   DASYNS010000237 DASYNS010000000
VERSION     DASYNS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2083064"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /locus_tag="VGP50_07445"
     CDS             <1..1678
                     /locus_tag="VGP50_07445"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEV8027249.1"
                     /translation="FLMHLPTAAEDSGAVLATGWRGLLVAGVVGGAALASFALVRRDL
                     QAKADPVVMRGLSLLLLFGLIFVNLAVLFVLPWSLAGGVWAASGVVILLLSLQLQQRL
                     SFAFALALQVIGGGAFVFGAYPALEELSPAGLTPLWHSGFWTPVAIALAAYIGAWLLF
                     RAERDQAKTLAAPLDDATLSLALLAWATVWWAFAWLSETFRFLPEEARPHAMLLVTAA
                     SVIVWMLAAELWQWRALAAFCGLSIPAALLALLLAVQPYYDPAEHLGFVAWPALLVAH
                     LVVLRRVAALMPEKWPNLLHIVGCWVFLGVAALEMRYAFIALSDNFNAWRWLGWASVP
                     ALYLLVMAQERVPAFWPFTAYEREYRAVAALPVAIILLAWFWLSNIVSDGAAAPLPYI
                     PLLNPLELGQLFALFALLIWLRTRFPMLPWAGALPAAAPYWLVGASALFLLTCGVARM
                     AHHWFGIPYEIEPLFAAMLVQAGLSIIWGVAALAAMVAGHLRQQRDLWIVGAALVAVV
                     VVKMFLIELFNAGGLPRIVSFIGVGVLLLIIGYFAPLPPKRADRFEEVAR"
     gene            1675..>2949
                     /locus_tag="VGP50_07450"
     CDS             1675..>2949
                     /locus_tag="VGP50_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 domain-containing protein"
                     /protein_id="HEV8027250.1"
                     /translation="MSATRSVVAGGRQRLLLQPREPALCGMVTLALGLAIALAGPAIA
                     AGEAPGDFAAKAPIELEGDGPYYQLALPFEAHFAAQFPDLRDLRVFNGKGETVPFSVI
                     REPTRSEQTSEQIALPWFPLYAPDTAPDAVPEIRVERRTDGAVVSVTGGGSKSDGGQK
                     LRGYLLDLSQSKSAAHRLALDWDPAMTGFQQVTVEASDDLQNWQTWRTAAQLARLEFN
                     GQHVDRRDIDLPDQHAGYLRLTWRAPAEAPVLTAVTLTAVSASYRPTPVVWSGALPPT
                     RTSDGAYEWDFPRLIAPERLKLGLPETNVLAPAELWGKGDGPPPSAWRLLAGTVVYRL
                     DVAGREWEQDDIALSGPPVRSIKLTVDARGGGLGNGVPTLSLGLAGRQLLFLARGGGP
                     FVLAIGNAKAELADLAPATLIPGYESSAAPPIS"
BASE COUNT          346 a         1031 c         1019 g          553 t
ORIGIN      
        1 gttcctcatg catttgccga cagcggccga ggacagcggc gccgtgctgg ccacgggctg
       61 gcgcgggctg ctcgtcgccg gcgtggtcgg cggcgcggcg ctcgcctcct tcgcgctcgt
      121 gcggcgcgat ctccaagcca aagccgatcc cgtcgtcatg cgcggcttgt cgctgctgct
      181 gctgttcggc ctgatcttcg tcaatctcgc ggtcctcttc gtgctgccct ggagtctggc
      241 cggcggcgtc tgggcggcga gcggcgtggt gatcctgctg ctgagtctcc agttgcagca
      301 gcggctgagc ttcgctttcg cgctcgcgct ccaagtgatc ggcggcgggg cgttcgtgtt
      361 cggcgcctat cccgcgctcg aggagctctc gcccgcagga ctgacgccgc tctggcacag
      421 cggattctgg acgccggtgg cgatcgcgct cgccgcctat atcggggcct ggctgctgtt
      481 ccgcgccgag agggaccagg cgaagaccct ggccgcgccg ctcgacgatg cgacgctgtc
      541 gctggcgctc ctcgcctggg cgacggtgtg gtgggcgttc gcctggttga gcgagacctt
      601 tcgcttcctg cccgaggagg cgcggcccca tgccatgctg ctggtgacgg cggcgagcgt
      661 catcgtctgg atgctggcgg cggagctctg gcaatggcgg gccttggccg cgttctgcgg
      721 cttgtcgatc ccggcggcgc tccttgcgct gctgctcgcc gtccagcctt attacgaccc
      781 ggccgagcat ctgggatttg tcgcctggcc ggcgcttctc gtcgcccatc tcgtcgtgct
      841 gcggcgcgtc gccgcgctca tgccggagaa gtggccgaac ctcctccata tcgtcggatg
      901 ctgggtgttc ctcggcgtgg cggcgctcga aatgcggtat gcgttcatcg ccttgagcga
      961 caacttcaac gcctggcgat ggctcggctg ggcgtctgtg cccgcgctct atctgctggt
     1021 catggcgcaa gagcgtgttc ccgccttctg gccgttcacc gcctatgagc gcgagtaccg
     1081 cgcggtcgcc gccttgccgg tggcgatcat ccttctggcg tggttctggc tcagcaacat
     1141 cgtgagcgac ggcgccgccg cacccttgcc ttatattccg ctgctcaatc cgctcgagct
     1201 gggccagctc ttcgcgctct tcgcgctgct gatctggctc aggacgcgct ttcccatgct
     1261 gccttgggcc ggagcgcttc cggcggcggc gccctattgg ctcgtcggcg cctcggcgct
     1321 cttccttctc acttgcggcg tggcccgcat ggcgcatcac tggttcggca tcccctatga
     1381 aatcgagccg cttttcgccg cgatgctcgt ccaggcgggc ctctcgatca tctggggcgt
     1441 ggcggcgctc gccgcgatgg tcgccggcca tctgcgccaa cagcgcgatc tctggatcgt
     1501 cggcgccgcc ttggtcgccg tggtcgtcgt caagatgttt ctcatcgagc tcttcaacgc
     1561 gggcggcctg ccgcgcatcg tctccttcat cggcgtcggc gtgctgctgc tcatcatcgg
     1621 ctatttcgcg ccgctgccgc ccaagcgggc cgacaggttc gaggaggtcg cgcgatgagc
     1681 gcgacgcgaa gcgtcgtcgc tggcgggcga cagcggttgc tcttgcaacc gcgagagccc
     1741 gcgctgtgcg ggatggtgac gctggcactc ggtctcgcga tcgctctcgc cggcccggcc
     1801 atcgccgccg gcgaagcgcc gggcgacttc gcggcaaagg cgccgatcga gctcgagggc
     1861 gacggcccct attaccagct cgccttgccg ttcgaggcgc attttgccgc gcagtttccc
     1921 gacctgcgcg atctccgcgt cttcaacggc aagggcgaga cggtgccgtt cagcgtcatt
     1981 cgcgagccga cgcgcagcga gcagacgagc gagcagatcg cgttgccctg gtttccgctg
     2041 tacgcgccgg acacggcgcc cgacgcggtt cccgagattc gcgtcgagcg ccgcaccgac
     2101 ggcgccgtcg tgtcggtgac gggcggcgga agcaagagcg atggcggcca gaagcttcgg
     2161 ggatatctgc tcgatctgag ccagagcaaa tccgcggcgc accgcctcgc cctcgactgg
     2221 gacccggcga tgaccgggtt ccagcaggtg acggtggagg cgagcgacga cctgcagaac
     2281 tggcagacct ggcggaccgc cgcgcagttg gcgcgcctcg aattcaacgg ccagcatgtc
     2341 gaccgccgcg acatcgatct cccggatcag catgcgggct atctccggct cacctggcgc
     2401 gcgccggccg aagcccccgt cctgaccgcc gtcacgttga cggccgtctc cgcgagctat
     2461 cgcccgactc ccgtcgtctg gtcgggcgcg ctcccgccga cgcgcacgag cgacggcgcg
     2521 tatgaatggg acttccctcg gctcatcgcg ccggagcggc tgaagctggg cttgccggaa
     2581 accaatgtgc tggcgccggc cgagctctgg ggcaagggtg atggaccgcc gccatcggcc
     2641 tggcggctgc tggcggggac cgtcgtctat cgcctcgacg tcgcgggccg cgaatgggag
     2701 caggacgaca tcgcgctgtc ggggccgccg gtccggtcga tcaagctcac cgtcgacgcg
     2761 cgcggcggcg gcctgggcaa cggcgtcccg accctgtcgc tcggtcttgc gggacggcag
     2821 ctgctgttcc tcgcgcgggg cggtggcccc ttcgtcctcg ctatcggcaa tgccaaggcc
     2881 gagctggcgg atctggcgcc cgccaccttg attccgggct atgagagctc ggccgcgccg
     2941 ccgatctcg
//
LOCUS       DASYNS010000238         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2240161, whole genome shotgun sequence.
ACCESSION   DASYNS010000238 DASYNS010000000
VERSION     DASYNS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2240161"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VGP50_07455"
     CDS             complement(<1..267)
                     /locus_tag="VGP50_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV8027251.1"
                     /translation="MTKPAFAGAIKDVDFAAALGERYLSYALSTITSRSLPDVRDGLK
                     PVHRRVLYAMRELNLDPKSGYKKCSRVVGDVVGKFHPHGNEAVYE"
     gene            complement(375..1118)
                     /gene="recO"
                     /locus_tag="VGP50_07460"
     CDS             complement(375..1118)
                     /gene="recO"
                     /locus_tag="VGP50_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV8027252.1"
                     /translation="MEWSDEGIVLGARRHGESALIVQLLTREHGRHLGLVRGGQGPKL
                     RGVFEVGNRLAARWKARLAEHLGHLSGELLRGHAAALLDDPARLACLSAAAALAESTL
                     PEREPHPRAFASLSALLDSLAADDGWAVRYVEWELTLLSELGFGLDLTRCAATGETAD
                     LIYVSPKSGQAVSAQAGAPYRDRLLALPRFLLPGSNAAAAPADVLDALALADHFLERR
                     VFLPHDHKMPASRARFVEIFQRMAKVSRA"
     gene            complement(1126..2031)
                     /gene="era"
                     /locus_tag="VGP50_07465"
     CDS             complement(1126..2031)
                     /gene="era"
                     /locus_tag="VGP50_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV8027253.1"
                     /translation="MSEAQAQRCGYVALVGAPNAGKSTLLNQLVGAKLSIVSPKVQTT
                     RSRLLGIVIAGEAQLIFVDTPGIFAPKRRLDRAMVAAAWAGADDADIVVLLVDAARSV
                     DADTDAIIARLKAAGRRAVLALDKVDLVAPAQLLPLADRLSREGIFDPVFMVSGLTGD
                     GVGDLRRHLAAALPEGPWLFPEDQLTDLPQRVMAAETTREQIFLQLHQELPYAATVET
                     ERWEERPDGSVRIEQVVHVQRASQKAIVLGKGGRQIKEIGARARAELEQVLGRRVHLF
                     LFVRVSEDWTEDRERYKAMGLDFES"
     gene            complement(2028..2720)
                     /gene="rnc"
                     /locus_tag="VGP50_07470"
     CDS             complement(2028..2720)
                     /gene="rnc"
                     /locus_tag="VGP50_07470"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV8027254.1"
                     /translation="MTDDSAAALAPLAEALGHAFAHPELLAEALTHPSARRGPRRSYE
                     RLEFLGDRVLGLIIAELLWRRFPQEAEGALTRRHTGLVRREALTEIARKIDLGRHMLL
                     STGEETAGARANPSVLADVCEAVIAALYLDGGLGAAQRFVERWWEPQLTKLGAPPHDP
                     KTALQEWAQARGRALPAYRTVATEGPAHRRVFTVTVTVDGVPPATASGSSKRAAETAA
                     AAAALAGLGDGT"
     gene            complement(2717..>2839)
                     /locus_tag="VGP50_07475"
     CDS             complement(2717..>2839)
                     /locus_tag="VGP50_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014413994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV8027255.1"
                     /translation="ENLVGRAEFIFFSTNGYASWWELWKWPFTIRYGRLLTAIH"
BASE COUNT          465 a          975 c          991 g          408 t
ORIGIN      
        1 ctcgtagacg gcctcgttgc catgcgggtg gaatttgccg acgacgtcgc cgacgacgcg
       61 cgagcatttc ttgtagcccg atttcgggtc gaggttgagc tcgcgcatgg cgtagagcac
      121 acggcgatgc accggcttca ggccgtcgcg cacgtcggga agcgagcgcg acgtgatggt
      181 cgagagcgcg taagagagat agcgctctcc caaggcggca gcgaaatcca catccttgat
      241 tgcgccggcg aaggcaggtt ttgtcataaa aaatcctgga ttgagttccc ctcaccctcc
      301 cgtcgctgcg cgacaggccc ctccctctcc cgtggtgcgg gagaggggct tttgggcgag
      361 ggactttgca aaaatcacgc tcgcgacacc ttagccattc gctgaaaaat ctcaacaaag
      421 cgggcgcggg aagccggcat cttgtgatca tgcggcagga agacgcggcg ctcgaggaaa
      481 tgatcggcaa gcgccagcgc gtcgagcacg tcggccggcg cggcggcagc gttgctgccg
      541 ggcagcaaaa agcgcggcag cgccaggagt cggtcgcgat agggcgcccc tgcctgcgcc
      601 gacacggcct ggcccgattt gggcgagacg tagatcaggt ccgccgtctc gcccgtcgcg
      661 gcgcagcgcg tgaggtcgag gccgaagccg agctccgaca gcaatgtgag ctcccattcg
      721 acatagcgga ccgcccagcc gtcatcggcc gcgagcgaat cgagaagcgc cgagaggctc
      781 gcgaaggcgc gcggatgagg ctcgcgctcc ggcagcgtcg actcggcgag cgccgccgcg
      841 gcgctcaaac acgcgagacg cgcgggatcg tcgaggagag cggccgcgtg accgcgcagc
      901 agctcgcccg agagatgccc cagatgctcg gcgaggcgcg ccttccagcg ggcggcgagg
      961 cggttgccga cctcgaagac gccgcgcagc ttgggcccct gcccgccgcg caccaagccc
     1021 aggtgccggc catgctcgcg cgtcaggagt tgcacgatga gcgcgctctc gccatggcgt
     1081 cgcgccccca gcacgatgcc ctcatcgctc cattccatcg ccgactcaac tctcgaaatc
     1141 gaggcccatc gccttgtagc gctcgcggtc ctcggtccaa tcctcgctca cccgcacgaa
     1201 gaggaacaga tggacgcggc ggcccagcac ctgctcgagc tcggcccggg cgcgcgcacc
     1261 gatctccttg atctggcgcc cgcctttgcc cagcacgatg gccttctggc tcgcgcgctg
     1321 cacgtgcacg acctgctcga tcctgacgct cccgtccgga cgttcctccc agcgctcggt
     1381 ctcgaccgtc gccgcatagg gcagctcctg gtgaagctga aggaagatct gctcgcgcgt
     1441 cgtctcggcc gccatgaccc gctgcggcag gtcggtgagc tggtcctcgg ggaagagcca
     1501 ggggccttcg ggcaaggcgg cggcgagatg acggcgcaga tcgccgacgc catcgcccgt
     1561 caggcccgac accatgaaca cgggatcgaa gatgccctcc cgcgacagcc ggtcggcgag
     1621 cggcaggagc tgcgccggag ccacgagatc gaccttgtcg agcgccagca cggcacggcg
     1681 tccggctgct ttgagccgcg cgatgatggc gtccgtgtcg gcatcgacgc tccgcgccgc
     1741 gtcgaccagg agcacgacga tgtcggcatc atcggcgccc gcccaggccg ccgcgaccat
     1801 cgcgcggtcg agccggcgct tgggtgcgaa gatgccgggc gtgtccacga agatgagctg
     1861 cgcctcgccg gcgatgacga tgccgagaag ccgcgagcgc gtcgtctgga ccttcggcga
     1921 gacgatcgag agcttcgcgc ccacgagctg gttgagcagc gtcgacttgc cggcattggg
     1981 cgcgcccacc agcgcgacat agccgcagcg ctgcgcctga gcctcgctca tgtgccgtcg
     2041 ccgagccccg ccaacgcggc agcggccgca gcggtctcgg cggcccgttt cgaggagccg
     2101 ctggcggtgg ccggcggcac gccgtcgacg gtgacggtga cggtgaagac gcggcgatgc
     2161 gccggtcctt cggtcgccac ggtgcgatag gccggcagtg cccggccgcg cgcctgggcc
     2221 cattcctgca aggccgtctt cggatcatga ggcggcgcgc cgagcttcgt gagctgcggc
     2281 tcccaccagc gctcgacgaa acgctgcgca gcgccgagcc cgccgtcgag atagagggcg
     2341 gcgatgacgg cttcgcagac atcggcgagc acgctgggat tggcgcgggc gcctgccgtc
     2401 tcctcgccgg tcgacagcag catatgccgg ccgaggtcga tcttcctggc gatctcggtc
     2461 aatgcctcgc ggcgcacgag gccggtgtgg cggcgggtga gcgcgccttc cgcctcttgc
     2521 gggaagcgcc gccacaagag ctcggcgatg atcaggccga ggacacggtc gccgaggaat
     2581 tcgagccgct catagctccg ccgcgggccg cggcgcgcgc tcggatgcgt gagcgcctcc
     2641 gccagcaatt cgggatgcgc gaaagcgtgg ccgagcgcct cggcgagcgg cgccaacgcg
     2701 gccgcagaat cgtccgtcaa tggatcgccg tgaggagccg accgtagcgg atcgtgaagg
     2761 gccacttcca gagctcccac cagctggcat agccgttggt cgagaaaaag atgaattcgg
     2821 cgcggccgac gagattctc
//
LOCUS       DASYNS010000239         8218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3176669, whole genome shotgun sequence.
ACCESSION   DASYNS010000239 DASYNS010000000
VERSION     DASYNS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3176669"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            351..1577
                     /locus_tag="VGP50_07480"
     CDS             351..1577
                     /locus_tag="VGP50_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027256.1"
                     /translation="MMAAATAAALLGGLSSARATDQVSLTIGKLLELTGPLSETGPSQ
                     DKAVSIAIDYANQSAAAAGVPLKITAVSADAQGDPQAALSAARALVAKGASAIVGPSI
                     TPESIAVANGLTIQKKVTLWPTGTAMRLRTIKDEGTIFRTVPPDSLQAYALAAALADK
                     MGDPKDKLLSIAYRNEPYGEGLAKGLVAAWTAKGGKVQGPVVFDPGQATFDSEAQQVV
                     DNNPDAYVVIDYPDTFAKFGASLVRTGKYDASKLVVPDALAFSTVPSNIPPQALEGAR
                     GTRGGSDMSTEAYKLFDQLWTKAGGVEHFALDANSFDSTTLVVLAAAAAHSADAGAIA
                     GKIHDVTRSGAPKFGPTKLGEALKAAWSGEPIDYVGVAGAFEFAANGDPTISRFDIYE
                     YKGGKLDVIKQIDASK"
     gene            1574..2584
                     /locus_tag="VGP50_07485"
     CDS             1574..2584
                     /locus_tag="VGP50_07485"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8027257.1"
                     /translation="MTRRLEAASGAPTGAPERRFESIPAAARLAAVLIAAAILMSFAR
                     GPIAVGQTTLNGLVSAGYLALGAVGLTLVFGTLRIINFAHGDLLTLGAYLTFGINALG
                     LPYWPAAAVAVALTALIAYALDFIIWEPMRRARTSTLQLFLVAIGLAIVIRYTIQFFA
                     GSQVRGVGQDVLTSVNLGPFHLGALQALVLVVGVVAIAAVGLALAFTRLGKQIRALSD
                     NMALAEVCGIDTRRAVRLIWLIAGGLAALAGVLYAAAVGSVNPNFGFFILLGLFTAAV
                     LGGIGSAYGALAGGIVIGLAQEWSTLFFNTRWKPVVGFAILIVMLLVMPRGIFGGGRA
                     RS"
     gene            2581..3516
                     /locus_tag="VGP50_07490"
     CDS             2581..3516
                     /locus_tag="VGP50_07490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8027258.1"
                     /translation="MSFFDLDFWVGVGIVAGIYGIFALGLQLNVGFTGLINLGQAGFM
                     CVGAYAMGMLVVDAGWPLGLAFPAAIVIAVAAGALVGLASLRLRADYFGIVTIAFSEI
                     VRYVFQNAAFAGGNQGIIGYDAEWRAFQSWASERLAAIGLGEAAEVPLLVAVWATLLL
                     CLAALRALQLSPWGRVLKAIREDEDAAAALGKNVFAFKLQSLGIAAALATIAGFFLAM
                     NVTYLYPSEFDPSFTFFGYAVLILGGFASYGGVVVGAVLLCALTEGLRFLDLPLSAGQ
                     AGSLRFIIVGLILICLSMFRPQGLLGSKAEMQLRA"
     gene            3513..4310
                     /locus_tag="VGP50_07495"
     CDS             3513..4310
                     /locus_tag="VGP50_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027259.1"
                     /translation="MTATRKAGEPILAVSDVTKRFGGLLAVDRATFAVAHGSITALIG
                     PNGAGKTTLLDLVSGYDRPDFGAVRFEGRRVDGLASYRVARRGVVRTFQLTRVFAAMS
                     VRDNMMLGAQRHPGEGLHRLAFSRAAVRRAEAAARERAERLLRAFALDAKASEYAGAL
                     SGGQRKLLEFARALMAKPRLVLLDEPMAGVNRALGAKLLDEVERLRREEGMTFLFIEH
                     DMDIVMQRADRVVVMAEGQVVAEGPPEFIRRDQRVIDAYLGRRGNAA"
     gene            4307..5059
                     /locus_tag="VGP50_07500"
     CDS             4307..5059
                     /locus_tag="VGP50_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027260.1"
                     /translation="MSEPSALAVADLVAGYSAEVDILKGVSIALAPREIVTVVGPNGA
                     GKSTLMKAIFGLIAPKAGRVMLAGESITGLRPSVIARRGVGYVPQRENVFPSLTVAEN
                     LEMGLAAAPNRPLNRRFDAMYALFPRLAERRRQKVDTMSGGERQMVAMARALMPEPSV
                     LLLDEPSAGLAPLLVDAMFEQIRAINREGITILMVEQNARAALALSDRGYVLDLGRNA
                     HEGRGREMIDDPRIAELYLGAAVPADNEGHPK"
     gene            5056..6057
                     /locus_tag="VGP50_07505"
     CDS             5056..6057
                     /locus_tag="VGP50_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV8027261.1"
                     /translation="MKAVVFDAHGPLDNMRYRDAPDPKPGPKDVLIRVRALALNGFDP
                     MVLRGIAGLKTPLPMTPGADIAGEIVALSAEVDRAHWKVGDRVTVIPNQDHGMVGETL
                     VGGASELFATESRYLLPVPDAVSFVDAACLPVAYGAALRMMETRAQLKAGEKVLILSA
                     AGGVGTCCVQLAKAAGCEVVACASSGTRAERLKGIGADHVVDTSQQNYVAEIHKLYGK
                     PRIRGGGGVDVIVNYTGGDTWAECFRALGRHGRLLTCGATAGFDPKTDLRYIWSFEFN
                     IIGCNGWTRDDQTELLNRVADGRLKPIKYCELPLERFGEAMTDLMERRVFGKAVLIP"
     gene            5970..6524
                     /locus_tag="VGP50_07510"
     CDS             5970..6524
                     /locus_tag="VGP50_07510"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV8027262.1"
                     /translation="MRAAAGALRRGDDRPDGTSGLRQGGADSMTTRAWKTPRGPAPAL
                     DFERLAAMVENCAFHKWLGVKLRALDDAGIAIEMPWRAEFESDPVVGYTHGGILASLI
                     DLAADYAVAARLGRGVPTVDMHVDYHRAAMPGPLVAHAAVVKLGGTLGTAEARIYDER
                     DNLIASGRALFMTRAPETTKEATT"
     gene            6521..7147
                     /locus_tag="VGP50_07515"
     CDS             6521..7147
                     /locus_tag="VGP50_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV8027263.1"
                     /translation="MIRLDHILWAAPDLDEGTKTIERLTGVTPARGGVHPGLGTRNSL
                     IALDPGVYFEIIAPDPAQDLAGNRGGHIAAQMRPGLMTFAIASDDLASLREAALHEKL
                     GVNGPVAMHRDTPSGGRLDWTILYLEDARFGEAIPFVIDWGKTPHPSASAPVGCRLKS
                     FAVAHPDAAALGRLYAALGVPTVVKGGPYPGFVAELATPKGDVVLTQS"
     gene            7185..>8218
                     /locus_tag="VGP50_07520"
     CDS             7185..>8218
                     /locus_tag="VGP50_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV8027264.1"
                     /translation="MTSADPSAGNLGYWCSASAARFPDKVALIDLSGDRPRETTYAEL
                     ERRLDRVAAMLTRLGLNRGDRFAISIGNCIEFVEIMFGAMRAGIVPVPLNTKLGPDTL
                     RYVIKDSGSAGAAVEPAANPHVADIVDSLGLDVRLLLDGAGLGWLGYEDALAESRADF
                     APPPLGDDDLAFLPYTSGSTGKPKGVALTHAGQCWWIGCLHKYWPTPAESRALAAVPL
                     YHKNAMAGAIKPMLSAGGSVVLLPDFEPRSFLKALAGYRCTHAGGVPAVFTLLLQERD
                     LIESLDFSELKGLKIGSAPTPKELLDAVEAAFGVPASESYGLTEGGPVMIGPPLDGRP
                     TPHGSCGVAWP"
BASE COUNT         1294 a         2833 c         2686 g         1405 t
ORIGIN      
        1 agccacggtt gagccactaa ggcaccgcca gacgaaaggg gcggaaacag acatgctcga
       61 cctaacgcca ccgcgccacg tctcgactct acgcacagcg gacgtcggga gtcggtagag
      121 gtttgcgtcc gcggggcggg aaacatacgt tgccttcgtt gcggcgggct ccttcctgct
      181 cgcccgaaaa gcgtctaagg ttacagccta atcgacaaga cgtcggctga accgctgttg
      241 cggcaagcgc tacttcgaaa ggcgttaggt ggaaacggag gcgcgcgatc gccgcctcat
      301 acgatgcgga gagtggtcag atgatcggac gtcggtccct gctgacggcg atgatggctg
      361 cggcgacggc tgcggcgttg ttgggcggat tgagttcggc tagggcgacc gaccaggttt
      421 cgctgaccat cggcaaactc ctcgaactca ctggacccct ctcagaaacc gggccgtcac
      481 aggacaaggc ggtcagcatc gcgatcgact acgccaacca gtccgccgcc gccgccggtg
      541 tccccctcaa gataaccgcc gtctccgccg acgcgcaggg cgatccgcaa gcggcgctgt
      601 ccgcggcgcg cgccctcgtc gccaaaggcg cctctgccat cgtcggcccg tcgatcacgc
      661 ccgaatcgat cgccgtcgcc aacggcctga ccatccagaa gaaggtcacc ttatggccga
      721 ccggtaccgc catgcgcctg cggacaatca aagacgaggg cacgatcttc cgcacggtgc
      781 cgccggatag cctgcaggcc tacgcgctcg ccgccgccct cgctgacaag atgggcgacc
      841 cgaaagacaa gctgctctcg atcgcctatc gcaacgagcc ctacggcgaa ggcctcgcta
      901 agggcctcgt cgccgcctgg acggccaagg gcggcaaggt acagggaccg gtcgtgttcg
      961 atccggggca ggcgaccttc gattcggaag cgcagcaggt cgtcgacaat aaccccgacg
     1021 cctatgtcgt gatcgactat cccgacacgt tcgccaagtt cggcgcctcc ctggtccgca
     1081 ccggcaagta cgatgcaagc aagctcgtcg tacccgacgc gctcgccttc tcgacggtgc
     1141 caagcaacat tccaccgcag gcgctcgagg gtgcgcgcgg cacgcgcggc ggctcggaca
     1201 tgtccaccga agcctacaag ctgttcgacc agctttggac gaaggcgggc ggggtcgagc
     1261 atttcgcgct cgacgccaac agtttcgact cgacgacgct cgtcgttctg gccgccgccg
     1321 ccgcgcattc ggcggacgcg ggcgccatcg ccggcaagat ccatgacgtc accagatccg
     1381 gcgcgccgaa attcggcccg accaagctcg gcgaggcgct gaaggcggcg tggagcggtg
     1441 agccgatcga ctacgtcggc gtcgccggcg cgttcgaatt cgccgccaac ggcgatccga
     1501 cgatcagccg cttcgacatc tatgaataca agggcggcaa gctcgacgtg attaagcaga
     1561 tcgacgcctc gaagtgacac gccgattgga agcggcgtcc ggcgcgccga cgggcgcgcc
     1621 ggagcgccgc tttgaaagca ttcctgcggc ggcgcggctc gccgccgtgc tgatcgccgc
     1681 cgcgattctg atgagctttg cgcgaggccc gattgcagtc ggccagacga cgctgaacgg
     1741 cctcgtatcg gccggctatt tggcgctcgg cgcggtcggc ttgacgctcg tcttcgggac
     1801 cttgcgtatc atcaatttcg ctcatggcga tctgctgacc ctgggcgcct atctgacctt
     1861 cgggatcaac gcgctcgggc tcccgtactg gccggccgcc gcggtcgcgg tcgccctgac
     1921 ggcgctcatc gcctatgcgc tcgatttcat catctgggag ccaatgcggc gcgcgcgcac
     1981 gagcacgctg cagctcttcc tggtcgccat cgggctcgcc atcgtgatcc gctacaccat
     2041 ccagttcttc gccggcagtc aggtccgtgg ggtcgggcag gacgtgctca cgagcgtgaa
     2101 cctggggccg ttccatctcg gcgcgctgca ggccctcgtg ctggtagtcg gcgtcgtcgc
     2161 catcgccgcg gtcggcctcg cgctcgcgtt cacgcgcttg ggcaagcaga ttcgcgcgct
     2221 ctccgacaac atggcgctgg ccgaggtttg cggcatcgac acgcgccggg ccgtaaggct
     2281 catctggctc atcgccggcg gcctcgcggc gctggcgggc gtgctctacg ctgccgcggt
     2341 cggctcggtg aacccgaact ttggcttctt catcctgctc ggcctcttca cagccgcggt
     2401 gctcggggga atcggcagcg cttacggcgc gcttgccggc gggatcgtca tcggcctcgc
     2461 gcaggaatgg tcgacgctat tcttcaacac tcgctggaag ccggttgtcg gcttcgccat
     2521 cctgatcgtc atgctgctcg tcatgccgcg cggcatattc ggcggcgggc gcgcgcgcag
     2581 ttgagtttct tcgacctcga cttctgggtc ggagtcggca tcgtcgccgg catctacggc
     2641 attttcgcgc tcgggctaca actcaatgtc ggcttcaccg gcctcataaa tctcggccag
     2701 gcggggttca tgtgcgtcgg ggcctacgcg atgggcatgc tggtcgtcga cgcaggttgg
     2761 ccgctcgggc tcgcctttcc cgcggcgatc gtcatcgccg tcgcggccgg cgcgctcgtc
     2821 ggcctggcca gcctgcgcct gcgcgccgac tatttcggca tcgttaccat cgccttctcc
     2881 gagatcgtgc gctatgtctt tcagaacgcc gccttcgccg gcggcaacca gggcatcatc
     2941 ggctacgacg cggagtggcg cgcgttccag tcctgggcct ctgagcgact cgccgcgatc
     3001 ggcctcggcg aggcggcgga ggttccgctg ctggtcgcgg tgtgggccac gctgctcctc
     3061 tgcctagccg cgctcagggc gcttcagctc agcccctggg gacgcgtgct caaggcgatc
     3121 cgcgaagacg aggacgctgc ggcggcgctc ggcaagaacg ttttcgcctt caagctgcag
     3181 tccctcggca tcgcggcggc gctggcgacg atcgccgggt tcttcctcgc catgaacgtc
     3241 acctatctct acccaagtga gttcgatccg agcttcacct tcttcggcta cgccgttctg
     3301 atcctcggcg gtttcgcgag ctacggaggc gtcgtcgtcg gtgcggtcct cctctgcgcg
     3361 ctgaccgaag gcctgcgctt cctcgaccta ccgctgtccg cgggccaggc gggctcgctc
     3421 cgattcatca tagtcggcct catcctcatc tgcctcagca tgttccgccc acaagggctg
     3481 ctcggcagca aggccgaaat gcagttgcgc gcatgacggc gacccggaaa gcgggcgagc
     3541 cgatcctcgc cgtgagcgac gtgaccaaac gcttcggcgg cctgctcgcc gtcgaccgcg
     3601 ctaccttcgc ggtcgcacac ggctcgatca ccgcgctcat cggccccaac ggcgcgggca
     3661 agacgacgct gctcgaccta gtcagcggct acgaccggcc cgattttggc gccgtgcgtt
     3721 tcgagggccg ccgcgtcgac ggcctcgcca gctaccgtgt cgcgcggcgc ggcgtcgtgc
     3781 gcactttcca gctcacgcgc gtcttcgctg cgatgagcgt gcgcgacaac atgatgctcg
     3841 gggcacagcg ccaccccggc gagggcctcc atcgcctcgc gttcagccgt gcggccgtga
     3901 gacgcgcgga ggccgcggct cgcgagcgcg ccgagcgcct tttgcgcgcc ttcgccctcg
     3961 acgccaaggc gagcgaatac gccggggcgc tgtcgggcgg gcagcgcaag ctgctcgaat
     4021 tcgcgcgggc gctgatggca aagccgcggc tcgtgctgct cgacgagccg atggcggggg
     4081 tcaaccgggc gctcggggcc aagctcctcg acgaagtcga gcgcctacgg cgcgaggagg
     4141 gcatgacgtt cctcttcatc gagcatgata tggacatcgt catgcagcgc gccgaccggg
     4201 tcgtcgtcat ggcggagggt caggtcgtcg ccgaaggccc tcccgaattc atccgtcgcg
     4261 atcagcgggt catcgacgcc tatctcggcc gcaggggaaa tgcggcgtga gcgaaccgtc
     4321 agcgcttgcg gttgcggatc tcgtcgccgg ctattccgcg gaggtcgaca tcctcaaggg
     4381 cgtatcgatc gcgctcgcgc cgcgagagat cgtcactgtc gtcggaccca acggcgcggg
     4441 caagtcgaca ctgatgaaag cgatcttcgg cctgatcgcg cccaaggcgg gacgggtcat
     4501 gctcgccgga gagtccatca ccggcctgcg tcccagcgtt atcgcgcggc gcggcgtcgg
     4561 ctacgtgccg cagcgggaga atgtttttcc cagtcttacg gttgcggaaa atctggagat
     4621 gggcttggct gccgctccga accgaccgct caatcgccgc tttgacgcaa tgtacgccct
     4681 ctttcccaga ctcgccgagc gccgccgcca aaaggtcgac acgatgtccg gcggcgagcg
     4741 ccagatggtg gcgatggcgc gcgcgctgat gcccgagccc tcggtgctgc tcctcgacga
     4801 gccctcggcc ggcctcgccc cgctcctggt tgatgcgatg ttcgagcaga tccgcgccat
     4861 aaaccgcgaa ggcatcacga ttctcatggt cgaacagaac gcacgggcag cgctcgcgct
     4921 ctccgaccgc ggatatgtcc tcgatctcgg ccgcaacgcc cacgaggggc gcgggcgaga
     4981 gatgatcgac gatccacgca tcgcggaact ctatctcggc gccgcggtgc ccgctgacaa
     5041 cgagggccat ccgaaatgaa agccgtcgtg ttcgacgccc acgggccgct cgataacatg
     5101 cgctatcggg atgcgccgga tccgaaacca ggtccgaaag acgtcttgat ccgggtgcgc
     5161 gccctggcgc tcaatgggtt cgaccccatg gtcctgcgag gcattgcggg cctgaagacg
     5221 ccgttgccca tgacgcctgg cgccgacata gccggcgaga tcgtcgcgct cagcgccgaa
     5281 gtggatcggg cgcactggaa ggttggggac cgcgtcacgg tcattcctaa ccaggaccac
     5341 ggcatggtcg gcgagaccct cgtcggcggc gcgagcgaac tcttcgcaac cgagtcgcgc
     5401 tatctcttgc ccgtacccga cgcggtgagc ttcgtcgacg cggcgtgcct gcccgtcgct
     5461 tacggcgccg ccctgcgcat gatggagaca cgggcgcaac tcaaggccgg cgagaaggtg
     5521 ctgatcctct cggcggcggg cggcgtcggc acttgctgcg tccaactcgc caaggcggcc
     5581 ggttgcgagg tcgtcgcctg cgccagctcg ggcacgcgcg ccgagcggct caagggtatt
     5641 ggcgccgatc acgtcgtcga tacttcccag cagaactatg tcgccgagat ccacaaactc
     5701 tatggcaagc cgcggatccg cggcggcggc ggcgtcgacg tcatcgtcaa ctatactggc
     5761 ggcgacacct gggccgagtg cttccgcgcg ctcggccgcc acggccgtct gctgacctgc
     5821 ggcgcgacgg cgggtttcga tccaaagacg gacttgcgct atatctggtc cttcgagttc
     5881 aatatcatcg gctgtaacgg ctggacgcgt gacgaccaga ccgaattgct taatcgtgtt
     5941 gcggatggcc gcctcaaacc gatcaagtat tgcgagctgc cgctggagcg cttcggcgag
     6001 gcgatgaccg acctgatgga acgtcgggtc ttcggcaagg cggtgctgat tccatgacca
     6061 cgcgcgcctg gaagactccg cgcggcccgg cgccggcgct cgatttcgaa cggctcgccg
     6121 ctatggtcga gaactgcgcc ttccacaaat ggctcggcgt taagctgagg gcgctcgacg
     6181 acgccggcat cgccatcgag atgccgtggc gcgcggagtt cgagtccgat ccggtagtcg
     6241 gctacaccca cggcggcatt ctcgcttcgc tgatcgacct cgccgccgac tacgccgtcg
     6301 cggcgcggct cgggcgcggc gttccgacgg tcgacatgca cgtcgattat catcgcgccg
     6361 ctatgccggg gccgctggtc gcgcacgccg cggtcgtaaa gctcggcggc acactgggaa
     6421 cagcggaggc gcgcatctac gacgagcgcg acaacctcat cgccagcggc cgcgcgctgt
     6481 ttatgacccg cgcgcccgag accaccaagg aggcgacgac gtgatccggc tggaccatat
     6541 cctctgggcg gcccccgatc tcgacgaggg cacgaagacg atcgagcgcc tcacgggggt
     6601 cactccggcg cgcggcggtg tccatcctgg ccttggcacg cgcaacagcc tcatcgccct
     6661 cgaccccggc gtctatttcg agatcattgc gccagatccg gcgcaggacc ttgccggcaa
     6721 tcgcggcggg cacatcgccg cacagatgcg cccgggcctg atgaccttcg ccatcgcctc
     6781 agacgacctc gcttcgctgc gcgaagcggc gctgcacgaa aagctcggcg tcaatggccc
     6841 agtcgctatg catcgcgaca cgccgagcgg cggacgtctc gactggacga tcctctatct
     6901 cgaggacgcg cgcttcggcg aagcgattcc cttcgtcatc gactggggga agacgccgca
     6961 tccctccgcg agcgcgccgg tcggttgccg tctcaaatct ttcgcagtgg ctcaccccga
     7021 tgctgccgcg ctggggcgtc tctacgccgc gcttggcgtt cccacagtgg tcaaaggcgg
     7081 gccctacccc ggattcgtcg cggagttggc gacgccgaag ggcgacgtcg tgctcaccca
     7141 gtcgtgacgc tgaggccgcc gacaagccaa acaaggatgc ggtcatgact tccgccgatc
     7201 ccagcgcagg aaatctcggc tactggtgca gcgcttcggc ggcacgcttc cccgacaagg
     7261 tcgcgctcat cgacctctcg ggcgatcggc cgcgcgagac gacctacgcc gaacttgagc
     7321 ggcgcctcga ccgcgtcgcg gccatgctga cgcgccttgg gctcaacagg ggcgatcgtt
     7381 tcgccatctc gatcggcaac tgcatcgagt tcgtcgaaat catgttcggc gcgatgcgag
     7441 cgggaattgt gcctgtgccg ctcaacacca agctcgggcc cgacactttg cgctatgtga
     7501 tcaaggactc gggctctgcc ggcgcggccg tcgagcccgc ggccaacccg catgtcgccg
     7561 acatcgtcga ctccctcggg ctcgacgtgc ggctcctcct cgacggcgcc gggctgggct
     7621 ggctcgggta cgaggacgca ctcgccgaga gccgggccga cttcgcgccg ccgccgcttg
     7681 gcgacgatga tctcgccttc ctgccctata cctcaggctc aaccggcaag cccaaaggcg
     7741 tcgccctgac gcacgccgga caatgctggt ggatcggctg cctgcacaaa tactggccga
     7801 cgcccgcgga gagccgcgct cttgccgcag tgccgctgta ccacaagaat gccatggccg
     7861 gcgcgatcaa gccgatgctg agcgccggcg gctccgtcgt gctgctgcca gattttgaac
     7921 cgcgaagttt tctgaaggcg ctcgcgggct atcgctgcac gcatgcgggc ggcgtgccgg
     7981 cggtgtttac gctgctcctt caggaacgcg accttatcga gagcctcgac ttctccgagc
     8041 tcaagggcct gaagatcggc tcggcaccga cgccgaaaga gctgctcgac gcggtcgagg
     8101 cggccttcgg cgttccggcc tccgagagct acggcctcac ggaggggggg ccggtgatga
     8161 tcgggccgcc gctggacggc cggccgacgc cgcacggcag ctgcggcgtt gcctggcc
//
LOCUS       DASYNS010000240         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1460207, whole genome shotgun sequence.
ACCESSION   DASYNS010000240 DASYNS010000000
VERSION     DASYNS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460207"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /gene="folK"
                     /locus_tag="VGP50_07525"
     CDS             complement(<1..343)
                     /gene="folK"
                     /locus_tag="VGP50_07525"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV8027265.1"
                     /translation="MILLGLGANLPSATHGPPPATLAAAIDALAADGVGVERRSSWYR
                     SAPVPAADQPWFVNGVIAVRTRLAPAELLALLHRTEQRFGRHRRHRNEPRVLDLDLLD
                     YQGRVESGASPV"
     gene            441..1004
                     /locus_tag="VGP50_07530"
     CDS             441..1004
                     /locus_tag="VGP50_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEV8027266.1"
                     /translation="MIFYPQEKIALFIDGANLYAAARALQFDIDYKRLLDVFGSKGRL
                     IRAFYYTALVEDQEYSPIRPLVDWLDYNGYTMVTKPTKEYTDATGRRKIKGNMDIELA
                     IDVMEMAQHLDHIVLFSGDGDFRRLVEAVQRKGVRVTVVSTIRSSPPMVADELRRQAD
                     MFVELQDLASSIMRVHSRDDQRQAASV"
     gene            1099..1740
                     /locus_tag="VGP50_07535"
     CDS             1099..1740
                     /locus_tag="VGP50_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV8027267.1"
                     /translation="MAEPGGDCPLCPRLAMFRTIQRAAYPDWHNAPVPSFGGEEARLL
                     IVGLAPGLRGANRTGRPFTGDFAGHLLYATLAKFGYARGRYGERADDGLTLVNCRITN
                     AVRCVPPENRPDLKEISACNPFLVGELKRLWRLKAVLALGSIAHQAVLVALGERRAAW
                     PFAHGAVHALSSGLLLADSYHCSRLNTNTGRLTPAMFEAVFEELGRRLGAPAS"
     gene            complement(1744..2247)
                     /locus_tag="VGP50_07540"
     CDS             complement(1744..2247)
                     /locus_tag="VGP50_07540"
                     /inference="COORDINATES: protein motif:HMM:NF023123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HEV8027268.1"
                     /translation="MTDWHVAQINVATALFPFDDPRIAEFMALLDAVNALAEQSPGFV
                     WRLKSDSGNATDLRVTEDPNFIVNMSVWNSVEALFAFVYKSSHRLVMAKRRQWFEPPV
                     RSYQALWWVAPGVFPAPQEGLDRLRHLDAHGPTAQAFTFKHKFPPPGVSGTPEDLRPD
                     PYCVGWE"
     gene            complement(2305..2766)
                     /gene="smpB"
                     /locus_tag="VGP50_07545"
     CDS             complement(2305..2766)
                     /gene="smpB"
                     /locus_tag="VGP50_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV8027269.1"
                     /translation="MALRGDERFVAQNRRARHDYHIESTLEAGIMLAGTEVKVLRKGL
                     ASINEAYATEREGDLYLVNAHFPEYPSARFNHEPGRPRKLLLRRREINRLMGAIKREG
                     ITLVPLAIYFNARGFAKLELGLAKGKRKADKRETEKQRDWQRQKARILRGD"
BASE COUNT          498 a         1021 c         1025 g          523 t
ORIGIN      
        1 ccacgggcga cgcgccgctt tcgacgcgac cctgatagtc gagaagatcg aggtcgagca
       61 cgcgcggctc attgcggtgg cggcggtgcc ggccgaagcg ttgctcggtc cgatgcaaca
      121 gcgccaagag ctcggccggc gccaatcgcg tcctgacggc gataacgccg ttgacgaacc
      181 aaggctgatc ggccgccggc accggcgccg agcgatacca ggacgagcgc cgctcgacgc
      241 cgacgccgtc cgccgcgagt gcgtcgatgg ccgcagccag cgtcgcgggt ggcgggccat
      301 gcgtcgcgct gggcaggttg gcacccaggc ccagcaggat catgaaacgg tcatggtggc
      361 gcttgacgac ttcgcgacat tacggacaaa tgctaaggaa ttgtgaagca cggctttgca
      421 cgtgtttgtg gagttgttgg atgatatttt atcctcaaga gaagatcgcc ttgttcatcg
      481 acggcgccaa cctctacgcg gcggcgcgcg ccttgcaatt cgacatcgat tataagcgtc
      541 tcctcgacgt gttcgggtcc aaagggcgcc tcatccgcgc cttctattat acggcgctgg
      601 tggaggatca ggagtactcg ccgatcaggc cgctggtcga ttggctcgac tacaacggct
      661 acaccatggt gaccaagccg accaaggagt acacggacgc tacggggcgg cgcaagatca
      721 agggcaacat ggacatcgag ctcgccatcg acgtgatgga gatggcgcag cacctcgacc
      781 acatcgtgct gttctcgggc gacggcgatt ttcgccggct ggtcgaggcc gtgcagcgca
      841 aaggcgtgcg cgtcaccgtc gtctcgacca tccgctcatc gccgcccatg gtcgcggacg
      901 aattgcgccg ccaggccgac atgttcgtcg agctgcagga tctggcgtcc tcgatcatgc
      961 gcgtccatag ccgcgacgac cagcgccagg cggcttccgt ctaaggcttg cacggacgac
     1021 accgcgccta tattccctct cccctcggga aagaggcgag acggggggca gccggtgacc
     1081 gcgaccacga ccgaggcaat ggcggagccg ggcggcgatt gtccgctctg cccgcgcctc
     1141 gccatgttcc gcaccattca acgcgcggcc tatccggact ggcacaacgc gcccgtccct
     1201 tccttcggcg gcgaagaggc gcggctgctg atcgtcgggc tggcgcccgg cctgcgcggc
     1261 gccaaccgca cgggacgacc tttcaccggc gattttgccg ggcacctcct ttatgcaacg
     1321 ctcgccaagt tcggctatgc ccgcggccgc tatggcgagc gtgccgatga cggcctgacg
     1381 ctcgtcaact gccgcatcac caacgcggtg cgctgcgtcc cgccggagaa ccggcccgac
     1441 ctcaaggaga tcagcgcctg caaccccttc ctcgtgggcg agctgaagcg cctctggcgc
     1501 ttgaaggcgg tgctggcgct gggctcgatc gcgcatcagg cggtgctggt ggcgctgggc
     1561 gagcggcgcg ctgcctggcc cttcgcgcat ggcgccgtcc atgcgctgtc gagcggcctc
     1621 ctgctcgccg acagctatca ctgctcgcgg ctcaacacca acaccggccg gctcacgccc
     1681 gcgatgttcg aggcggtgtt cgaggagctc ggccgccggc tcggcgcccc ggcgagctga
     1741 accctattcc cagccgacgc agtagggatc gggacgcagg tcctcgggcg tgccggacac
     1801 gccgggcggc gggaatttat gcttgaaggt aaaggcctgc gccgtcggcc cgtgcgcgtc
     1861 gagatggcgg agccggtcga gcccctcttg cggcgccggg aagacgccgg gcgccaccca
     1921 ccacagcgcc tggtaggagc ggaccggcgg ctcgaaccat tgccggcgct tcgccatgac
     1981 gaggcggtgc gacgacttgt agacgaacgc gaagagggcc tcgacgctgt tccacaccga
     2041 catgttgacg atgaagttcg gatcctccgt cacccgcaga tcggtggcgt tgccgctgtc
     2101 ggatttaagg cgccagacaa agccgggcga ctgctcggcc agggcgttga ccgcatcgag
     2161 cagcgccatg aactcggcga ttcgcgggtc atcaaaggga aacagggcgg ttgcgacatt
     2221 gatctgcgcg acatgccagt cggtcatcgg agcctccgcg gcgctggtgt gattttagcc
     2281 tgcctgccga cccggcgcac ccgctcagtc gccgcggagg atgcgcgcct tctggcgctg
     2341 ccagtcgcgc tgcttctcgg tttcgcgctt gtcggctttg cgcttgcctt tggcgaggcc
     2401 gagctcgagc ttggcgaagc cgcgcgcatt gaaatagatg gccagcggca ccagcgtgat
     2461 accctcgcgc ttgatcgcac ccatgaggcg gttgatctcg cggcggcgca gcaggagctt
     2521 gcgcggccgt cccggctcat gattgaagcg cgccgaggga tactcgggaa aatgcgcgtt
     2581 gacgaggtag agatcgccct cccgctcggt ggcgtaggcc tcgttgatgc tggccagccc
     2641 tttgcgcagc accttcactt cggtgccggc gagcattatc ccggcctcga gcgtgctctc
     2701 gatgtggtag tcgtgccgcg cccgccgatt ctgcgcgacg aaacgttcgt ccccgcgcag
     2761 tgccatctta gcccggattt ctcccacgga catgcgctgc gacggcgatc tttggcgcca
     2821 gggtaggagc gcggcgcgca gcgatgcggg acatcggaag cggcgcgcga cgccccctgg
     2881 cgccaaagag cgccgtccct ccgggttgca cagtgttggc gcgctgccgc gtcgcgaagc
     2941 cttgacgatg tcccgcatcg cctgcggctt cgctcctaac atcgcgccaa cactgcgcaa
     3001 cgcagcgcgt gtccgtggga gaaatccggg ctaagcctac tggtgacgcc agcgctcgat
     3061 gatcttg
//
LOCUS       DASYNS010000241         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3646200, whole genome shotgun sequence.
ACCESSION   DASYNS010000241 DASYNS010000000
VERSION     DASYNS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3646200"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            698..1690
                     /locus_tag="VGP50_07550"
     CDS             698..1690
                     /locus_tag="VGP50_07550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8027270.1"
                     /translation="MKDLSGKIAVITGGGTGMGRELARQLVAEGCNVAICDISADTMA
                     ETKRLSESDRPPQGVRITTHIADVSDEAQILRFRDEVAEQQSTGEIHLLFNNAGIGGG
                     GSMLTDTREQWDKTFTVCWGGVYLTTRAFLPMLQKAREARIVNTSSVNGFWASLGPNV
                     PHTAYSAAKFAVKGFTEALMNDLKINAPHIKCSLVMPGHIGTSIVANSRKILSGGGDA
                     LTPAEIERARLRMSTNGTDVSRLSDQDIQNRWDERVRRFQEEAPTTAAAAAAIILDGV
                     KAGRWRILVGGDAQRLDELVRRDPEHAYDDDFFETMAAETGWQLGTGARGGGQR"
     gene            complement(1712..2509)
                     /locus_tag="VGP50_07555"
     CDS             complement(1712..2509)
                     /locus_tag="VGP50_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV8027271.1"
                     /translation="MSSTYTATDAEAYERLMGRWSRLLAEDFLAFAGLARGESVLDLG
                     CGTGALSLALAARAEPRSILGIDIAERYVDHARRRSSDPRLGFAVGDAAALDFADDRF
                     ERSLSLLALNFVPQPARALAEMRRVTREGGIVAAAVWDFAGGLVYQRIFWDTAAALDP
                     EADRARARHFASPLTREGELEAAFAATGLRDVESRALSIRMRYADFADYWEPIANAQG
                     PVGDYVKRLPAAALATLAAALEHAYRSGGADGPRSMAATAWIARGRV"
BASE COUNT          496 a          881 c          896 g          392 t
ORIGIN      
        1 caatccgcag ggagtagctt tgcggattct ccacggcgca ggcatagatc ggagtcccgc
       61 aattcccgca gaaggcggcc agcgagatcg ccactcgccg gcggtcgatg ctcatgaaga
      121 gtagggaggg ctcaagggga agaagggccg ccctcgggcg gcccttccct tgtcagatag
      181 cggccagtca gcgaccgcga ctcatcgctt caagcgacgg cgatccctgc atgggcagcg
      241 atcccggtca cggtgctcga ccgcaatctt tgacaatatg acacgggatc acctcctttc
      301 gtggttacgg ggaaaaccac cctaaacgcc gcggcaccgc aaaacaagag gcagatcccg
      361 gtccgccggc ggcgtcagcc tttgtcggcg gaaggcactg cggcgaggct gtccccccaa
      421 ggcagtgccg ggggagtagc tctgcggatt ctccacggcg caggcataga tcgtagtccc
      481 gcaattccca cagaaagcgt ggcggcgctt gttgccgcgg cagatgcccg ccgccgcagg
      541 atcgacctcc gcctcgaagg tgatctcgcc gcaatggcag cgtccatcga tcttcatggg
      601 accctccgtc ggcccggagc ttgccgttta ccgtccagca ccgtagcgca tccagcgccc
      661 ttgggctaag actgggcgca tcgaggagat cgacgccatg aaggatcttt ccggcaagat
      721 cgccgtcatc accggcggcg gcacgggcat gggacgtgag ctggcgcgcc agctcgtcgc
      781 cgagggctgc aatgtcgcca tatgcgatat ctccgcggac acgatggcgg aaacgaagcg
      841 cctcagcgag agcgaccggc cgccgcaagg cgtgcgcatc accacgcaca tcgccgacgt
      901 atcggacgag gcgcagatcc ttcgcttccg cgatgaggtc gccgaacagc agagcaccgg
      961 cgagattcat ttgctcttca acaatgcagg catcggcggc ggcggcagta tgctcaccga
     1021 cacgcgcgag cagtgggaca agaccttcac cgtctgttgg ggtggcgtct atctcacgac
     1081 tcgcgctttc ctgcccatgc tgcagaaggc ccgcgaagcg cgcattgtca acacgagcag
     1141 cgtcaacggc ttctgggcga gcctcggccc caacgtgccg cacaccgcct atagcgcggc
     1201 gaaattcgcg gtgaagggtt tcaccgaggc gctgatgaac gatctcaaga tcaacgcgcc
     1261 gcatatcaag tgctcgctcg tcatgccggg gcatatcggc acctcgatcg tcgccaattc
     1321 gcgcaagatc ctgagtggcg gcggcgacgc cttgaccccg gcggagatcg agcgcgcgcg
     1381 gctgcgcatg agcaccaatg gcacggatgt gtcgcgcctc tcggaccagg acatccagaa
     1441 ccgctgggac gagcgggtgc gccgcttcca ggaggaagcg ccgaccactg ccgccgcggc
     1501 tgcggcgatc attctcgacg gagtcaaggc gggacgctgg cgcattctcg tgggcgggga
     1561 cgcgcagcga ctcgacgagt tggtccggcg ggatccggaa cacgcctatg acgacgattt
     1621 cttcgagacc atggccgctg agaccggttg gcagcttgga acgggagcgc ggggtggcgg
     1681 gcagcgctga gcccgcgctc agagccgcgc gtcagacgcg gcctcttgca atccaggcgg
     1741 tcgccgccat gctgcgcggg ccgtctgcgc cgcccgagcg gtaggcgtgc tccagcgcgg
     1801 cggcgagagt ggcgagagcg gctgccggca gccgcttcac atagtcgccg accggcccct
     1861 gggcgttggc gatcggttcc caatagtcgg cgaaatcggc atagcgcatg cggatcgaca
     1921 gcgcgcgcga ctcgacatcg cgcagcccgg tcgccgcgaa ggccgcttcg agttcgccct
     1981 ctcgggtcag cgggctcgcg aagtgacgag cgcgggcgcg atcggcctcg ggatcgagag
     2041 cggcggcggt atcccagaag atacgttgat agacgagccc tccggcgaag tcccacacgg
     2101 cagcggcaac gatgccgccc tcgcgcgtga cccgccgcat ttcggcgagc gcgcgcgcgg
     2161 gctgcggaac gaagttgagg gcgaggagcg acagcgagcg ctcgaaacga tcgtcggcga
     2221 agtccagagc agcggcgtcg ccgactgcaa agcccaatcg cggatcactc gagcggcgcc
     2281 gcgcatgatc gacatagcgc tctgcgatgt cgatgccgag gatgctgcgc ggctcggcgc
     2341 gggcggcgag cgccagtgac aacgcaccgg taccgcagcc gagatcgagg acgctttcgc
     2401 cccgagcaag tcccgcgaag gcgaggaaat cctcggcgag cagccggctc cagcgtccca
     2461 tcagccgctc gtaagcttcc gcatccgtgg ccgtatatgt cgaggacatc gctgcacccg
     2521 tcgacgcaag gaaccttgtg cagcaagatc gccgacaccg gcggggccgt caaatccatc
     2581 gatcccgcgg acgcgataac gtgacagctc acgcaacggc agataacgga cggcccggcg
     2641 ctgccgctca tgcgcggcgt cgccg
//
LOCUS       DASYNS010000242         8147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_680838, whole genome shotgun sequence.
ACCESSION   DASYNS010000242 DASYNS010000000
VERSION     DASYNS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8147
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680838"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGP50_07560"
     CDS             complement(<1..206)
                     /locus_tag="VGP50_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027272.1"
                     /translation="MALVKTSKIAAATTKSRQPTTMVEPAAPAVKDNGNRAHMPPRRA
                     GATARVGVATPPWSAKPQAQPPIVV"
     gene            complement(516..2651)
                     /locus_tag="VGP50_07565"
     CDS             complement(516..2651)
                     /locus_tag="VGP50_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV8027273.1"
                     /translation="MIDYEVGADGIAVIAWNMADRPMNVLNDASVAAFAERVNAAIGD
                     ATVKGVVITSKKRDFLAGADLTVMGRDVDAASLTTFTQGFHALLRGMEKSGKPFCAGI
                     NGTALGGGYEIALACHRRIAADLPKTEIGLPEVTIGLLPGGGGTQRLPRLIGIRNALP
                     LLLEGRKLDPRKALTAGLVDEVVPAERLLDAAKAWLLGDGVKNPTQPWDRKGYQIPDG
                     PVLGRKGNETFVVGNAMLHARTYGNYPAARNIASCVYEGLIVDIDNGLKIESRYFVNC
                     ALSPEARNMIRSLFFSIGDANKLASRPKAVPTQVWRRIGVLGAGMMGAGIAHVSALAG
                     IEVVLLDTTLAAAEKGKAHSRGLLDKRVQQQRMNEAQRDEVLARIAPTTDFAALAGCE
                     MVVEAVFEERKIKADVTKRAEAVLGKDAIFASNTSTLPITGLAEASIRPENFIGLHFF
                     SPVDRMALVEIIRGGRTSDACLARSMDYIKQIRKTPITVNDARGFYTSRVFGTYVSEG
                     LTLLAEGVSPALIDNAGRIAGMPVGPLALADEVSLDLMVKVRRQTKADLGAAYEGNAR
                     SEALIDLMVDKLGRLGKKGGKGFYDYPADGGKKRLWPGLAKHFPPAAEQPDLDEVKRR
                     LLYVQSIETARCLEENVVIDPRDADVGAILGWGFPPFLGGTVSQMHSIGVDRFVAECD
                     RLAQRHGKRFAPPKLLRDMAAGGRAFYTA"
     gene            complement(2654..3862)
                     /locus_tag="VGP50_07570"
     CDS             complement(2654..3862)
                     /locus_tag="VGP50_07570"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEV8027274.1"
                     /translation="MTETFIYDAVRTPRGRGKASGSLYSVTPMQLAVTALQAVRDRNA
                     LDTSAVDDVVLGCVEPVGEQGADIARVAVLEAGFAETTAGVQINRFCASGLEACNMAA
                     AQVMSGQSDMAIGGGVESMSRVPMGSSGGAWPSDPQVAFHTYFVPQGISADLIATKYG
                     MSRTDVDAYAVESQKRAVRAWAERRFARSVVPVKDINGLTVLDRDEHIRADTTMQSLA
                     SLEPSFAGPGENFGFDAVAIQRYPEVERIEHVHHAGNSSGIVDGAAAVLIANKAIGDK
                     LGLKPRARIRAFASIGSEPAIMLTGPSYSAEKALKRAGMSVGDIDLFELNEAFAAVVL
                     RFMQVLDIPHDKINVNGGAIAMGHPLGATGAMILGTLLDELERTGKSTGLATLCVGAG
                     MGTATIIERV"
     gene            4154..5296
                     /locus_tag="VGP50_07575"
     CDS             4154..5296
                     /locus_tag="VGP50_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEV8027275.1"
                     /translation="MGPLAGLKIIEMAGIGPAPMCAMLLADLGATVLRIERREPSGLG
                     LPGHPRFQILNRSRHAIAVDLKQAEGVGLLLRLVEAADALIEGFRPGVMERLGLGPEA
                     CFARNPKLVFGRMTGWGQEGPLAGAAGHDLNYIALAGVLHSIGRAGQPPTPPLNLVGD
                     FGGGALYLAMGLLAALLEAQRSGKGQVVDAAMVDGAASLMAIFYAMHAAGMFSLNRGE
                     NVLDSGAFFYDVYECADGRYVALAPIEDKFLEEFLRRVEIDAAAMPPKLDRSRWPEAK
                     AKLAARFKTKSREEWCRLLEGSDACFAPVLSLAEAPSHPHNRARGTFIEIDGIVQPAP
                     APRFSRTPPAAPTPPETPGARGIASLARWGIAADTIGRLQNAGVLS"
     gene            5313..5750
                     /locus_tag="VGP50_07580"
     CDS             5313..5750
                     /locus_tag="VGP50_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV8027276.1"
                     /translation="MTLTLAQAALIVDKALEHGRALKLKPLTVAVLDAGGHLVAFKRE
                     DGASILRPQIAQGKAWGVLGMGFGGRELARRAAAAPVFVSALMDASQGRVIPVPGGVL
                     IRGGEGGIVGAVGISGDTSEQDEACAVYGIKAAGLAADTGDPG"
     gene            6064..7521
                     /locus_tag="VGP50_07585"
     CDS             6064..7521
                     /locus_tag="VGP50_07585"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HEV8027277.1"
                     /translation="MSQISLSGPTRIGTRRTRDGSRIIHHEALSLSIGLLRAIDIAGV
                     PVAGLIAYWTRYQQLTVEFRHALIIILGMAVVANVMTIVGAYDIAELRAPRTQLFKVV
                     GGWGVSIAILLAIAFFDKITDQYSRLWIGYWFVIGVALSGAARVAVGTYLGRRRRAGN
                     LSIDVAIVGCEPFARQVMQQITWPGELEVRVVGTFAPYLSAPPGTGLAADATVDGLLR
                     LARKTHIDEIIVHLPEKRDAEFATMVHKLGELPVNVNLCPDLSDLPISPRKLTILRDA
                     FMINVFERPLSGWSSVLKRAEDILLSGALLLFCLPLMLLVALAIKFDSKGPVFFRQLR
                     FGFNNNPITVLKFRTMVTDAANDPTARQARRGDPRVTRVGRFLRQTSLDELPQLVNVL
                     MGDMSLIGPRPHAIAHNEYYAAIIDGYLGRHRVKPGITGWAQVNGLRGETATVDAMHD
                     RVKHDLYYIENWSLALDCWILVRTLAVGFVHRNAY"
     gene            7655..7837
                     /locus_tag="VGP50_07590"
     CDS             7655..7837
                     /locus_tag="VGP50_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027278.1"
                     /translation="MTGPRLQLDASAMALMVVLCACRGVKQVANSGISPVLQAGLRAA
                     RANRAPLVATAVAPAP"
     gene            7834..>8147
                     /locus_tag="VGP50_07595"
     CDS             7834..>8147
                     /locus_tag="VGP50_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027279.1"
                     /translation="MIVADAAPRFRWSAAALYRLLLLWLAGIDLRLTILAVPPVLPLI
                     HRDLALDEKSVAALTGLPVLLFGILAVPGSLLIARIGARRALILGLLTVAIASALRGV
                     GPS"
BASE COUNT         1227 a         2766 c         2770 g         1384 t
ORIGIN      
        1 actactatcg gtggttgcgc ctgcggtttg gcggaccatg ggggcgtcgc cacgcccacg
       61 cgcgcggtag cccctgcgcg acgcggcggc atgtgagcgc ggttgccgtt atccttaacc
      121 gccggagccg cgggctcgac catcgtcgtc ggctgtcgtg acttggtcgt cgcggcagca
      181 atcttcgacg tctttaccag cgccatcggt ttctccggga gttgcgatgg cgcagcttaa
      241 accttggcga cggggagcgc gagatatcaa tcgggtgtga cggaaggcaa cttctgcgat
      301 taacgatgcc gcgctgcggg gattgtaatg accggccgat tgcaatgatc ggccgcggct
      361 cgttgcattg tcggcggtgc catgcggccg ttcgtaccgt cgaaggactt cgagacgagc
      421 aagcgtttct ataccgatct cggcctcagc cctcgtccgc tcggcgcgga tctggcgaaa
      481 gcggcgttgg gatgggatag aagctctgcc cgtcatcacg ccgtgtagaa agcgcgcccc
      541 ccggccgcca tgtcgcgcag cagcttcggc ggcgcgaagc gcttgccgtg gcgctgcgcg
      601 aggcggtcgc attcggcgac gaagcggtcg acaccgatgc tgtgcatctg actcaccgtg
      661 ccgccgagga acggcgggaa accccagccg aggatggcgc cgacatcggc gtcgcgggga
      721 tcgatgacga cattctcctc gaggcagcgc gcggtctcga tcgattgcac atagaggagc
      781 cgccgcttca cttcgtccag gtccggctgc tcggcggcgg gcgggaaatg tttggcgaga
      841 ccgggccaga ggcgcttctt cccgccatcg gcgggataat cgtagaagcc ctttccacct
      901 ttcttgccga gccggccgag cttgtcgacc atgaggtcga tcagcgcctc ggatctggca
      961 ttgccctcat aggcggcgcc gagatccgcc ttggtctggc ggcgcacctt gaccatgaga
     1021 tcgagcgaga cttcgtcggc gagcgccagc ggcccgaccg gcattccggc gatgcggccg
     1081 gcattgtcga tgagcgccgg gctcacgcct tcggcgagca gcgtcaggcc ttcgctgaca
     1141 tacgtgccga agacgcggct cgtatagaag ccgcgggcat cgttcacggt gatcggcgtc
     1201 ttacggatct gcttgatgta atccatcgag cgggcgaggc aggcatcgct ggtgcggccg
     1261 ccgcggatga tctcgaccag cgccatgcga tcgaccggag agaagaagtg gaggccgatg
     1321 aaattctccg ggcggatgct cgcctcggca aggcctgtga tcggcagcgt cgaggtgttg
     1381 gaggcgaaga tcgcgtcttt gccgagcacg gcctcggcgc gcttcgtcac gtcggctttg
     1441 atcttgcgct cctcgaacac cgcctcgacc accatctcgc agccggcgag agcggcgaaa
     1501 tcggtggttg gtgcgatacg cgccaggact tcgtcgcgct gcgcctcgtt catgcgctgc
     1561 tgctggacgc gcttgtcgag aaggccgcgc gaatgcgcct tgcctttctc ggccgcggca
     1621 agcgtcgtgt cgagcagcac cacctcgatg ccggcgagcg cgctcacatg ggcgatgccg
     1681 gcgcccatca tgccggcgcc gagcacgccg atcctgcgcc agacctgtgt cggcacggct
     1741 ttcggccgcg acgccagctt gttggcgtcg ccgatcgaga agaagaggct gcggatcatg
     1801 ttgcgcgcct cgggcgagag cgcgcaattg acgaaatagc ggctctcgat cttgaggccg
     1861 ttgtcgatgt cgacgatcag gccctcatag acgcaggacg cgatgttccg cgccgccgga
     1921 taattgccat aagtgcgggc gtgcagcatg gcattgccga cgacgaaggt ctcgttgccc
     1981 ttgcgcccga gcacgggacc gtccgggatc tggtagccct tccggtccca aggctgcgtc
     2041 gggttcttga caccgtcgcc gagcagccag gccttggccg cgtcgagcag gcgctccgcc
     2101 ggcaccactt cgtcgacgag gccggcggtg agcgcctttc tgggatcgag cttgcgtccc
     2161 tcgagcagga gcggcagcgc gttgcggatg ccgatgaggc gcggcaggcg ctgcgtgccg
     2221 ccgccgccgg gcagcaggcc gatggtcacc tcgggcaggc cgatctcggt cttcgggaga
     2281 tcggcagcga tccggcgatg gcaggcgagc gcgatctcgt agccgccgcc cagtgccgtg
     2341 ccgttgatgc ccgcgcaaaa gggcttgccc gacttctcca tgccgcgcag cagcgcatgg
     2401 aaaccttgag tgaaggtggt gagagacgcg gcgtcgacgt cgcggcccat gacggtgaga
     2461 tcggcgccgg cgaggaagtc gcgcttcttc gaggtgatga cgacgccttt gacggtcgca
     2521 tcgccgatcg ccgcattgac gcgctccgcg aacgccgcga ccgaggcgtc gttcaagacg
     2581 ttcatcggcc ggtccgccat gttccaggca atgacggcga tgccgtctgc gccgacctca
     2641 tagtcgatca tggctatacc cgctcgatga tcgtcgcggt gcccatgccg gcgccgacgc
     2701 agagcgtggc aaggccggtg ctcttgccgg tgcgctcgag ctcgtcgagc agcgtgccga
     2761 ggatcatggc gccggtggcg cccaagggat ggcccatggc gatggcgccg ccattgacgt
     2821 tgatcttgtc atgggggatg tcgagcacct gcatgaagcg cagcacgacg gcggcgaagg
     2881 cctcgttgag ctcgaagagg tcgatgtcgc cgacgctcat gccggcgcgc ttcagcgcct
     2941 tctcggccga ataggagggg cccgtgagca tgatagcggg ctcgctgccg atcgaggcga
     3001 aggcacggat gcgggcgcgc ggcttcaggc ccagcttgtc gccgatcgcc ttgttggcga
     3061 tcagcaccgc ggcggcgccg tcgacgatgc cggaggaatt gccggcgtga tggacgtgct
     3121 cgatgcgctc gacctcggga tagcgctgga tcgccaccgc atcgaagccg aaattctcgc
     3181 ccggtccggc gaaggagggc tcgagcgaag ccagggactg catggtggtg tcggcgcgga
     3241 tatgctcgtc gcggtcgagc acggtgaggc cgttgatgtc cttcaccggc accaccgacc
     3301 tggcgaagcg ccgctccgcc caggcgcgca cggcgcgctt ctggctctcg acggcgtagg
     3361 cgtcgacgtc ggtgcggctc atgccgtatt tggtggcgat gaggtcggcc gaaatgcctt
     3421 gcggcacaaa ataggtgtgg aaggcgacct gcggatcgct gggccaggcg ccgcccgacg
     3481 agcccatggg cacgcggctc atgctctcga cgccgccgcc gattgccatg tcgctctgcc
     3541 cggacatgac ttgcgccgcg gccatgttgc aggcctcgag gccgcttgcg cagaagcggt
     3601 tgatctggac gccggccgtg gtctcggcga agcccgcctc gagcacggcg acgcgagcga
     3661 tatcggcgcc ctgctcgccc accggctcga cgcaaccgag cacgacatcg tcgacggcgg
     3721 aagtgtcgag cgcgttgcgg tcgcgcaccg cctggagcgc ggtcacggca agctgcatcg
     3781 gcgtcacgct gtagagcgag cccgaggcct tgccgcggcc gcggggcgtg cgcacggcgt
     3841 catagatgaa ggtttcggtc atggcgtcct ccgcacggtt tctgccgagc atagccgcca
     3901 gcgaatgcgg cgccaacgca accgcagaca tgcctcccat gaatccgctc gcggaggatc
     3961 gggctgcgcg ataaggtcgg cgtcgcaatc catggctgac gctccctcac cctcccactg
     4021 cttcgcagtg ggtccctccc tctcccgcat tgcgggagag gggctatttt tccctcgccc
     4081 gcgagagcgg gagagggagg ggcccggcgc gaagcgccgg gagggtgagg gaacgcctcc
     4141 gagcatctga ccagtgggac cgctcgccgg actgaagatc atcgagatgg ccgggatcgg
     4201 gccggcgccg atgtgcgcga tgctgctcgc ggatctcggc gccacggtgc tcaggatcga
     4261 gcggcgtgag ccgagcggcc ttggactccc cggccatccg cgcttccaga tcctcaatcg
     4321 cagccggcat gcgatcgccg tcgacctgaa gcaggcggag ggcgtcggct tgttgctgcg
     4381 cctcgtcgag gccgcggatg cgctcatcga gggcttccgc cccggcgtga tggagcggct
     4441 gggcctgggt cccgaggctt gcttcgcgcg gaacccgaag ctcgtcttcg gccgcatgac
     4501 cggctggggc caggaggggc cgctcgccgg ggcggccggg catgatctca actacatcgc
     4561 gctcgccggc gtgctccact cgatcggccg cgccggccag ccgccgacgc cgcccctcaa
     4621 cctggtcggc gatttcggcg gcggcgcgct ctatctcgcc atgggcctcc tcgctgcgct
     4681 tctcgaggcg cagcgctcgg gcaagggtca ggtcgtcgac gccgcgatgg tggacggcgc
     4741 ggcatcgctg atggcgatct tttacgcgat gcatgcggcc ggcatgttct cgctcaaccg
     4801 gggcgagaac gtgctcgaca gcggcgcatt cttctacgac gtctacgaat gcgccgacgg
     4861 ccgctatgtc gcgctggccc cgatcgagga caagttcctc gaggagttcc tgcgccgcgt
     4921 ggagatcgac gcggcggcga tgccgcccaa gctcgaccgc agccgttggc ccgaggcgaa
     4981 ggccaagctc gcggcgcgct tcaagacgaa gagccgcgag gaatggtgcc gattgctcga
     5041 aggcagcgac gcctgcttcg cgcccgtgct ctcgctggca gaggcgccga gccacccgca
     5101 taatcgcgcg cgcggcacct tcatcgagat cgacggcatc gtgcagccgg cgccggcgcc
     5161 gcgcttcagc cgcacgcccc cggcggcgcc gacaccgccc gagacaccgg gcgcgcgcgg
     5221 catcgcctcg ctcgcccgat ggggcatcgc cgccgatacc atcggtcggc tgcagaatgc
     5281 cggcgtgctg agctgacccc cgggagcaac ctatgacact caccctcgcc caggccgcgc
     5341 tcatcgtcga caaggcgctc gagcacgggc gcgccctcaa gctgaagccg ctgacggtgg
     5401 cggtgctcga cgccggcggt catctcgtcg cgttcaagcg cgaggacggc gcgagcatcc
     5461 tccggccgca gatcgcgcaa ggcaaagcct ggggcgtgct gggcatgggc ttcggcgggc
     5521 gcgagctcgc ccgccgcgcc gcggccgctc ccgtcttcgt gagcgcgctc atggacgcgt
     5581 cacaaggacg cgtcatccct gtgccaggcg gcgtgctgat ccgcggcggc gaaggcggca
     5641 tcgtcggcgc cgtcggcatc agcggcgaca cgtccgagca ggacgaggcc tgcgcggtct
     5701 acggcatcaa ggcggcgggc ctcgccgccg acaccggcga tcccggctga agaaggtcaa
     5761 cgcggtctcg acgcgagcgc cgcatcaccg ggccgcgtga cgcgttgtca ctcgggagtc
     5821 agcagcgtgc tcgcgatcac gcgctgcggt tgcgcagcat catcgctgat ttcgtcaatc
     5881 ggaacttttg tcgcccagcg gtgttggaga agcgttgcca ttcggccaca gctcgcgcct
     5941 ttccacccaa tcgatcatcg ccgcgacgac ggagacgtgc cttgtagcgt tcgcacatcg
     6001 gccgagctga gacaatgcgc ggcgatcgat ttcactgaag cgaaggacgg gtggacagaa
     6061 tccatgtcgc aaatttctct ctcgggtccg acccgcatcg gcacaaggcg cacgagagac
     6121 ggctcgcgca tcatccacca cgaggccctg tcgctctcga tcgggctttt gcgcgcgatc
     6181 gacatcgccg gcgtgccggt cgccggcctc atcgcctatt ggacacgtta ccagcaactc
     6241 acagtcgaat tccgccacgc cttgatcatc attctgggca tggcggtcgt cgccaacgtc
     6301 atgaccatcg tcggcgccta tgacatcgcc gagctgcgtg cgccgcgcac ccagctcttc
     6361 aaggtggtcg gcggctgggg cgtcagcatc gccatcctcc tggcgatcgc ctttttcgac
     6421 aagatcaccg atcagtattc gcgtctttgg atcggctact ggttcgtcat cggcgtcgcg
     6481 ctgtcgggcg cggcgcgagt ggcggtcggc acctatctcg gccggcgccg gcgcgcgggc
     6541 aacctctcga tcgacgtcgc catcgtcggc tgcgagccct tcgcccgcca ggtgatgcag
     6601 cagatcacct ggccgggcga gctcgaggtg cgcgtcgtcg gcaccttcgc gccctatctc
     6661 tcggcaccgc ccggcaccgg ccttgccgcc gacgcgaccg tggacgggct gctgcggctc
     6721 gcgcgcaaga cgcatatcga cgagatcatc gtgcacttgc ccgagaagcg cgacgccgag
     6781 ttcgccacga tggtgcacaa gctcggcgag ctcccggtca acgtcaatct ctgtcccgac
     6841 ctttccgacc tgccgatctc gccgcggaag ctcaccattt tgcgcgacgc cttcatgatc
     6901 aacgtgttcg agcgcccgct ctccggctgg tcgtccgtgc tgaagcgggc cgaggacatc
     6961 ctcctgagcg gcgcgctgct gctcttctgc ctgccgctga tgctgctggt ggcgctcgcc
     7021 atcaagttcg acagcaaagg gccggtgttc ttccgccagc tgcgcttcgg cttcaacaac
     7081 aatccgatta ccgtgctcaa attccgcacc atggtgacgg acgcggcaaa cgatcctacc
     7141 gcgcgccagg cgcgccgcgg cgatccgcgc gtgacgcggg tcgggcgctt cctgcgccaa
     7201 accagcctcg acgagttgcc ccagctcgtc aatgtgctga tgggcgacat gtcgctgatc
     7261 gggccgcggc cccacgccat cgcccataac gagtattacg ccgccatcat cgacggctat
     7321 ctggggcgtc accgcgtcaa gcccggcatc accggctggg cgcaggtgaa cggcctgcgc
     7381 ggcgagaccg ccacggtcga cgccatgcat gaccgcgtca agcacgacct ctactacatc
     7441 gagaactggt cgctcgccct cgattgctgg atcctggtcc gcaccctcgc cgtcggcttc
     7501 gtccaccgca acgcctactg agggcggtcc cctcgccctc ccaacgcttc gcgttgggcc
     7561 cctccctctc ccacaagggg agagggattt cttcgatgct ggcgcggcaa ggaaacggtt
     7621 gcgccggacg gcgcggcggg cgaggttgcg ccgcatgacg ggaccacggc tgcaactcga
     7681 tgcgagcgcg atggcgctga tggtggtgct ctgcgcctgc cgcggcgtca agcaggtcgc
     7741 caatagcggc atttcgcccg tcctccaggc aggattgcgc gccgcccgag cgaatcgcgc
     7801 tcctctcgtg gcaaccgcgg tcgcgccggc gccatgattg ttgccgatgc cgcgccgcgg
     7861 ttccgctgga gcgccgccgc cctctatcgc ttgttgctac tgtggctcgc cggcatcgac
     7921 ctgcgcctga cgattctggc ggtgccgccg gtgctgccgc tgatccaccg cgatctcgcg
     7981 ctcgacgaga aatccgtcgc cgcgctcacc ggactgccgg tgctgctctt cgggatcctc
     8041 gccgtcccgg gatcgctgct gatcgcgcgg atcggcgcgc gccgcgcgct catcctgggg
     8101 ctgctgaccg tcgccatcgc ctcggcgctc cgcggcgtcg gcccgtc
//
LOCUS       DASYNS010000243         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1928642, whole genome shotgun sequence.
ACCESSION   DASYNS010000243 DASYNS010000000
VERSION     DASYNS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928642"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1971
                     /locus_tag="VGP50_07600"
     CDS             <1..1971
                     /locus_tag="VGP50_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8027280.1"
                     /translation="EPGVGKTAIVEGLALRIVNGDVPESLHDKKLLALDLGALIAGAK
                     YRGEFEERLKAVLQEVTGAAGAYILFIDEMHTLIGAGKADGAMDASNLLKPALARGEL
                     HCIGATTLDEYKKHVEKDAALARRFQPIFVEEPSVEDTISILRGLKDKYEAHHGVRIT
                     DGALVAAATLSHRYITDRFLPDKAIDLVDEASARLKMQVDSKPEELDTIDREVIRLKI
                     EQEALKKERDPGSRARLGGLETELAALEKKSADLTARWKSEKEKLSDAQKLRNELDQL
                     RVELANAQRRGEFQRAGEISYGRIPEIEKKLKAMEAGEGKGAMVEEAVTPNHIAQVVS
                     RWTGIPVDRMLEGEKEKLLRMEEQIGKRVVGQAEAVKAVSSAVRRARAGLQDPHRPIG
                     SFMFLGPTGVGKTELTKALAEFLFDDEAALLRIDMSEYMEKHSVARLIGAPPGYVGYE
                     EGGALTEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGHTVDFRNTLIV
                     MTSNLGAELLVNQPEGQDTDAVRDQVMAIVRASFRPEFLNRVDEIILFHRLQRAEMGH
                     IVDIQMGRLAKLLEDRKIAITLEPKAREWLADKGWDPAYGARPLKRVIQKSVQDPLAE
                     LILAGRIKDGEKVVISAGKQGLKFNGEVAAAA"
     gene            complement(2134..>2433)
                     /locus_tag="VGP50_07605"
     CDS             complement(2134..>2433)
                     /locus_tag="VGP50_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027281.1"
                     /translation="RLLFPARMIADHIGISTNIVEFWLTPWRRTDEHLPMSHIAEVAH
                     TRGFVWDTISVESSGGLNPLVVDGLPKAPARYFVEHVRERINEAAAATPPLQPRH"
BASE COUNT          462 a          797 c          822 g          352 t
ORIGIN      
        1 gagccgggcg tcggcaagac cgcgatcgtc gagggcttgg cgctgcgcat cgtcaatggc
       61 gacgtgccgg aaagcctcca cgacaagaag ctcctggcgc tcgatctcgg cgcgctcatc
      121 gccggcgcca aatatcgcgg cgaattcgag gagcggctca aggcggtgct gcaagaagtc
      181 accggagcag ccggcgccta catcctcttc atcgacgaga tgcacacgct catcggcgcc
      241 ggcaaggccg atggcgccat ggacgcttcg aacctcctta agcccgcgct cgcccgcggc
      301 gagctgcatt gcatcggcgc caccacgctc gacgaatata aaaagcacgt cgagaaggac
      361 gcggcgctcg cccggcgctt ccagccgatc tttgtcgagg agccgagcgt cgaggacacg
      421 atctcgatcc tgcgcggcct caaggacaaa tacgaggcgc atcacggcgt gcgtatcacc
      481 gacggcgcgc tggtcgcggc ggcgacgctc tcgcaccgct acatcaccga ccggttcctg
      541 cccgacaagg cgatcgacct ggtcgatgag gcctcggcgc ggctgaagat gcaggtcgat
      601 tccaagccgg aagagctcga taccatcgat cgcgaggtca tccggctcaa gatcgagcag
      661 gaggcgttga agaaggagcg cgatcccggc tcgcgcgccc gcctcggcgg gctcgagacc
      721 gagctcgcag cgctggagaa gaaatccgcc gatctcaccg cgcgctggaa gtcggagaag
      781 gaaaagctct ccgacgcgca gaagctgcgg aacgagctcg accagctgcg cgtcgagctc
      841 gccaacgccc agcgccgcgg cgaattccag cgcgccggcg agatctccta tggccgcatc
      901 cccgagatcg agaagaagct caaggcgatg gaggcggggg agggcaaggg cgcgatggtc
      961 gaggaggcgg tcacgcccaa tcacatcgcc caagtggtct cgcgctggac cggcattccg
     1021 gtcgaccgca tgctcgaagg cgagaaggaa aaactcctgc gcatggagga gcagatcggc
     1081 aagcgcgtcg tcggccaggc cgaggccgtc aaagccgtgt cgagcgcggt gcgccgcgcc
     1141 cgcgccggct tgcaggaccc gcaccggccg atcggctcgt tcatgttcct cgggccgacc
     1201 ggcgtcggca agaccgagct caccaaggcg ctcgccgaat tcctgttcga cgacgaggcg
     1261 gcgctgctgc gcatcgatat gtccgaatac atggagaagc actcggtcgc gcggctgatc
     1321 ggcgcgccgc ccggttatgt cggttacgag gaaggcggcg cgctcaccga agccgtgcgc
     1381 cggcggccct accaggtggt gctgttcgac gagatcgaga aggcgcaccc ggacgtgttc
     1441 aacgtgctgc tgcaggtgct cgacgacggc cggctcaccg acgggcaggg ccacaccgtg
     1501 gatttccgca acacgctgat cgtgatgacc tcgaatctcg gcgccgagct tttggtcaac
     1561 cagccggaag gccaggacac cgacgcggtg cgcgatcagg tgatggcgat cgtgcgcgca
     1621 agcttccgcc cggaattctt aaaccgcgtc gacgagatca tcctgttcca ccggctgcaa
     1681 cgggcggaga tggggcacat cgtcgacatc cagatggggc ggctcgccaa gctcctggaa
     1741 gaccgcaaga tcgcgatcac gctcgagccg aaagcgcgcg aatggctcgc cgacaagggc
     1801 tgggatccgg cctatggcgc gcgcccgctc aagcgcgtga tccagaagtc ggtgcaggac
     1861 ccgctcgccg agctcattct cgccgggcgc atcaaggacg gcgagaaggt ggtgatctcg
     1921 gccggcaagc agggtctcaa attcaacggc gaggtcgccg ccgcggcgta gcctgctatc
     1981 tcgtcatgcg cgggctcgac ccgcgcatcc atgccgactt gcggtttact taaagcgcgc
     2041 caacgcgatg agagtgccaa cgcgatcggc gcaagcgtcc gcgccgcatg gattgccggg
     2101 tcaagcccgg ccatgacgcc ctgagacaga cgatcaatgg cgcggctgca gcggcggcgt
     2161 ggcggccgcc gcttcgttga tccgctcgcg cacgtgctcg acgaaatagc gcgccggcgc
     2221 tttcggcagc ccgtcgacga ccagcggatt gagcccgccc gagctttcga cgctgatcgt
     2281 gtcccagacg aagccacggg tgtgcgccac ctcggcgatg tgcgacatcg gcaaatgctc
     2341 gtcggtgcgc cgccacggcg tcagccagaa ctcgacgatg ttggtcgaga tgccgatatg
     2401 gtcggcgatc atccgggcgg ggaacaggag ccg
//
LOCUS       DASYNS010000244         5908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1150257, whole genome shotgun sequence.
ACCESSION   DASYNS010000244 DASYNS010000000
VERSION     DASYNS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150257"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /gene="nuoF"
                     /locus_tag="VGP50_07610"
     CDS             <1..1276
                     /gene="nuoF"
                     /locus_tag="VGP50_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEV8027282.1"
                     /translation="KDRIFTNLYGLHDWRLAGARARGDWDTTRDLVLKGRDWITTEVK
                     NSGLRGRGGAGFPTGVKWSFMPKESARPTYLVVNADESEPGTCKDRDIIRHDPHKLVE
                     GCLLASVGMAVHHCYIYIRGEFYNEAQNLQAAIDEAYAAGLIGKNACGSGYDFELYLH
                     RGAGAYICGEETALLESLEGKKGMPRLKPPFPAAVGLYGCPTTVNNVETIAVVPTILR
                     RGASWFAGIGRPKNEGTKVYCISGHVEKPCNVEEAMGIPLKELLERYAGGVRGGWDNL
                     LGVIPGGSSVPVIPKSVSETVLMDFDSLRDAKSGLGTAAVIVMDKSTDIIKAIARLSK
                     FYKHESCGQCTPCREGAGWMWRVMERMVKGNASVEEIDMLLEVSYEIEGHTICALGDA
                     AAWPVQGLIRHFRPEMERRIREYREAASRRAA"
     gene            1438..3507
                     /gene="nuoG"
                     /locus_tag="VGP50_07615"
     CDS             1438..3507
                     /gene="nuoG"
                     /locus_tag="VGP50_07615"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEV8027283.1"
                     /translation="MPKLTVDGIQVEVPQGATVLQACQLAGVEVPHFCYHERLAIAGN
                     CRMCLVEQEKAPKPIASCAMPAGEGMVIHTESEKAKKARHGVMEFLLINHPLDCPICD
                     QGGECDLQDQAMAYGFDRGRYLENKRAVKDKDFGPLVATSMNRCIHCTRCVRFLTDVA
                     GVEELGAVGRGENMEIDTYIEHSLTSELSANIIDLCPVGALTSKPYAFAARSWELGKT
                     ESVDVLDAVGSNIRVDSRGAQVMRVLPRLNEEINEEWISDKTRFAVDGLTRRRLDRPY
                     VRKSGKLVEVEWGEAFAVIARHLKGVPGSRIAALAGDLADCEAVMALKDLMAALGSPN
                     LDCRQDGAVLDASCRASYLFNTTIAGIDRADACLLVGTNPRIEAPIIHARLRKRWLQG
                     GFKVAAIGPELHLALPVTPLGLGPETFREVVEGRHPWAETLKAAKNPMLVLGQGALAR
                     KDGAAILDLARRLADSGNMVRPEWNGFNVLQVAAGRVGGLDLGFVPGAGGRDIAGILA
                     GCESGEIAALYLLGIDEIDESWCGKTFVIYQGHHGDKGAGRADVVLPGAAYTEKDATY
                     VNTEGRPQLARRAVFPPGDARDDWKILRALSEALGHRLPYDSLAQLRRRMTEVNPVFA
                     ATDRVLPAAWGSFGKAGSLEPAPFEYAFAEFYRTDPISRASPTMAACAETMAAGGRPL
                     AKTGTHG"
     gene            3500..4522
                     /gene="nuoH"
                     /locus_tag="VGP50_07620"
     CDS             3500..4522
                     /gene="nuoH"
                     /locus_tag="VGP50_07620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEV8027284.1"
                     /translation="MAELWTGYGRPTAITIAEILAIVVPLLLAIAYLTYAERKVLAAA
                     QLRKGPNVVGPFGLLQPLADGAKLLFKETVIPSGANRVVFIAAPIITFTLSLVAWAVI
                     PFDAGVVVANINVGILYLFAISSLGVYGIIMAGWSSNSKYAFLGALRSAAQMVSYEVS
                     MGFVIVTVLLCAGSLNLSAIVAAQRHVWFCIPLFPMFIVFFVSALAETGRSPFDLPEG
                     ESELVAGYFVEYSSMSFALFYLGEYGNMILMSAMTSILFFGGWLAPFNLQPFTGALAS
                     LWGMAWFALKIAFVLFCFLWVRATFPRFRYDQLMRLGWKVFLPLSLGWLVVTAGFLVG
                     MGWLPA"
     gene            4737..5225
                     /gene="nuoI"
                     /locus_tag="VGP50_07625"
     CDS             4737..5225
                     /gene="nuoI"
                     /locus_tag="VGP50_07625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEV8027285.1"
                     /translation="MAFLDRGARSVLLWELVQGLWLTLKYMFQPPVTVNYPYEKGPLS
                     PRFRGEHALRRYPNGEERCIACKLCEAICPALAITIEAEPREDGSRRTTRYDIDMTKC
                     IYCGLCQEACPVDAIVEGPNFEFATETREELFYDKDKLLANGDRWEAELAKNIALDAP
                     YR"
     gene            5233..5847
                     /locus_tag="VGP50_07630"
     CDS             5233..5847
                     /locus_tag="VGP50_07630"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEV8027286.1"
                     /translation="MIIQAICFWLFSFVLVGCSVMVVSARNPVHSVLFLILAFFNAAA
                     LFLLLGAEFLALILVIVYVGAVAVLFLFVVMMLDIDFAELRRGFITYLPIGALIGLML
                     FLELFLAFAAWTVAPDVAKSIAAPTPALSGLTNTQALGALIYTRYVYAFQTAGMVLLV
                     AMVGAIVLTLRARVGVRRQKIAVQLARRRQETLAIVKVQPRQGI"
BASE COUNT          937 a         1977 c         1910 g         1084 t
ORIGIN      
        1 caaggaccgg atcttcacga atctctacgg cttgcatgac tggcggctcg ccggcgcgcg
       61 cgcgcgcggc gactgggata cgaccaggga tttggtcctc aaggggcgcg attggatcac
      121 caccgaggtg aagaattcgg ggctgcgcgg gcgcggcggc gccggctttc cgaccggcgt
      181 caaatggtcc ttcatgccca aggagagcgc gcggccgacc tatctcgtgg tcaatgccga
      241 tgaatccgag ccgggcacct gcaaggaccg cgacatcatc cgccacgatc cgcacaagct
      301 cgtcgagggc tgcttgctgg cgagcgtcgg catggccgtc catcattgct acatctatat
      361 ccgcggcgag ttctacaacg aggcgcagaa cctccaggcg gcgatcgacg aggcctatgc
      421 ggccgggctc atcggcaaga atgcctgcgg ctcgggctat gatttcgagc tctatctcca
      481 tcgcggcgcc ggcgcctata tctgcggcga ggagacggcg ctgctcgaga gcctcgaggg
      541 caagaagggc atgccgcgcc tgaagccgcc cttcccggcg gcggtcggcc tttacggctg
      601 cccgaccacg gtcaacaacg tcgagacgat cgcggtggtg ccgaccatcc tgaggcgcgg
      661 cgcctcgtgg ttcgccggca tcgggcggcc caagaacgaa ggcacgaagg tctattgcat
      721 ctcgggccat gtcgagaagc cctgcaacgt cgaagaggcc atgggcattc cgctgaaaga
      781 gctgctcgag cgctatgcgg gcggcgtgcg cggcggttgg gacaatcttc tcggcgtcat
      841 ccccggcggg tcgtcggtgc cggtaatccc gaaatcggtc tcggagaccg tgctcatgga
      901 tttcgactcg ctgcgcgatg ccaagagcgg cctcggcacc gccgcggtga tcgtcatgga
      961 caagtccacc gacatcatca aagcgatcgc gcggctctcg aaattctaca agcatgagag
     1021 ctgcggccaa tgcacgcctt gccgcgaagg cgccggctgg atgtggcggg tgatggagcg
     1081 catggtgaaa ggcaacgcgt cggtcgagga gatcgacatg ctgctcgagg tctcctacga
     1141 gatcgaaggc catacgatct gcgctttggg cgacgccgcg gcatggcccg tgcaaggcct
     1201 gatccgccac ttccggccgg agatggagcg gcgcatccgc gagtaccggg aggcggcatc
     1261 acgccgggct gcttagtgct tacctccgca aatccgagcc atacccccca ccccaaccct
     1321 ccccctcaag gggggagggg aaaacgccgt cagctcccat cttccccctc cccccttgag
     1381 ggggagggag gggtgggggg tggcgccccg ctttctccag gtcgcgcgta ggacgccatg
     1441 ccgaagctca ccgtcgatgg gatccaggtg gaggtgccgc aaggcgccac cgtgctccag
     1501 gcctgccaac tcgcgggcgt cgaggtcccg catttctgct accacgagcg gctcgcgatc
     1561 gccggcaatt gccgcatgtg cctcgtcgag caggagaagg cgccgaagcc gatcgcgtcc
     1621 tgcgcgatgc cggcgggcga agggatggtc attcatacgg aaagcgagaa ggccaagaag
     1681 gcccgtcacg gggtgatgga gttcctgctc atcaatcacc cactcgactg ccccatctgc
     1741 gatcagggcg gcgaatgcga tctgcaggat caggccatgg cctatggctt cgaccgcggc
     1801 cgctacctgg agaacaagcg ggcggtcaag gacaaggatt tcggccctct ggttgccacc
     1861 agcatgaacc gctgcatcca ttgcacgcgc tgcgtccggt ttctgaccga tgtggcggga
     1921 gtcgaggagc tgggtgccgt cggccgtggc gagaacatgg aaatcgacac gtatatcgag
     1981 cattcgctca cttccgagct ctcggccaac atcatcgacc tctgcccggt gggcgctctg
     2041 acctcgaaac cttatgcctt cgccgcgcgc tcctgggagc tcggcaagac ggaatccgtc
     2101 gacgtgctcg acgccgtcgg cagcaacatc cgcgtcgaca gccggggtgc ccaggtgatg
     2161 cgcgtgctgc cgcgcctcaa cgaggagatc aacgaggagt ggatctccga caagacgcgc
     2221 tttgccgtcg acggcttgac gcgccgccgc ctcgatcggc cctatgtgcg caagagcggc
     2281 aagctcgtcg aggtcgaatg gggcgaggcc ttcgcggtca tcgcccggca tctcaaaggc
     2341 gtgccgggaa gccgcatcgc ggcgctcgcc ggcgatctcg ccgattgcga agcggtcatg
     2401 gcgctcaagg acctcatggc ggcgctgggc tcgcccaatc tcgattgccg ccaggacggc
     2461 gccgtgctcg acgcgtcctg tcgcgcgagc tacctcttca acaccaccat cgccggcatc
     2521 gatcgcgccg atgcctgcct ccttgtcggc accaatcccc gcatcgaggc gccgatcatc
     2581 catgcgcggc tgcgcaagcg ctggctccag ggcggcttca aagtcgcggc catcggcccc
     2641 gagctccatc tcgctttgcc ggtcacgccg ctcggcctcg gacccgagac cttccgcgag
     2701 gtcgtcgagg ggcgccatcc ctgggccgaa acgctcaaag cggcaaagaa tccgatgctg
     2761 gtgctggggc agggggcgct cgcgcgaaag gatggcgctg ccatcctcga cctcgcgcgt
     2821 cgtctcgccg attccggcaa catggtgcgc ccggagtgga acggcttcaa cgtgctccag
     2881 gtcgcggcag ggcgcgtcgg cgggctcgac ctgggcttcg ttccgggcgc cggcggtcgc
     2941 gacatcgccg gcattctcgc cggctgcgag agcggcgaga tcgcggcact ctatctcctc
     3001 ggcatcgacg agatcgacga gagctggtgc ggcaagacct tcgtcatcta ccagggccat
     3061 cacggcgaca agggcgcggg tcgcgccgac gtcgtgctcc cgggcgccgc ctataccgag
     3121 aaggacgcga cttacgtcaa caccgagggg cgtccccagc tcgctcgccg cgccgtcttc
     3181 ccgccgggcg atgcgcgcga cgactggaag atcctgcgtg ccctctccga agcgctgggg
     3241 catcggctgc cctatgattc gctggcgcag ctgcgccgcc gcatgaccga ggtcaacccg
     3301 gtcttcgccg ccaccgaccg cgtcctgccg gcggcctggg ggagcttcgg caaggcgggg
     3361 agcctcgaac ccgcgccgtt cgaatacgct ttcgccgaat tctatcgcac cgaccccatc
     3421 agccgcgcat cgccgaccat ggccgcctgc gccgagacga tggccgcggg cgggcggccg
     3481 ctagcgaaga cgggcacgca tggctgagct ctggactggc tacggccgcc cgacggccat
     3541 caccatcgcc gagatccttg cgatcgtcgt gccgctgctg ctggccatcg cctatctcac
     3601 ctatgccgag cgcaaagtgc tggcggcggc gcagctgcgt aaaggaccga atgtcgtcgg
     3661 gcctttcggc ctgctgcagc ctctcgccga cggcgccaag ctcctcttca aggagacggt
     3721 gatcccgagc ggcgccaacc gcgtcgtctt catcgccgcg ccgatcatca ccttcacttt
     3781 gagcctcgtc gcctgggccg tcattccctt cgatgccggc gtcgtcgtcg ccaacatcaa
     3841 tgtcggcatc ctctatctct tcgccatcag ctcgctcggc gtctacggca tcatcatggc
     3901 gggctggtcg tcgaactcga aatacgcctt cctgggcgcg ttgcgctcgg ctgcgcagat
     3961 ggtctcctac gaagtctcca tgggcttcgt catcgtcacg gtgctgctct gcgccgggtc
     4021 gctcaatctc tcggcgatcg tcgcggccca gcgccacgtc tggttctgca tcccgctctt
     4081 tccgatgttc atcgtcttct tcgtctcggc gctggccgag acgggccgct cgcccttcga
     4141 cctgccggaa ggcgaatcgg agctggtggc ggggtacttc gtcgaatact cgtcgatgtc
     4201 ctttgcgctc ttctacctcg gcgaatacgg caacatgatc ctgatgagcg cgatgaccag
     4261 catcctgttc ttcggcggct ggctcgcgcc cttcaacctc cagcccttca ccggcgcgct
     4321 cgcctcgctt tggggcatgg cctggttcgc actcaagatt gccttcgttc tcttctgttt
     4381 cctctgggtg cgggcgacct ttccgcgctt ccgctacgac cagctgatgc ggctcggctg
     4441 gaaggtgttc ctgccgctgt cgctgggctg gctggtggtg acggcagggt tcctcgtcgg
     4501 catgggctgg ctgccggcat aggaccgcaa tcaaatgcaa cgccgatccc ttccatcgtc
     4561 attcccgcga aagcgggaat ccagcgccgt cggtacgtcg cttgcccttg gatccccgct
     4621 ttcgcgggga cgacgtgttt gtgcgatgtc agccgaaaga accagggaac gtcggcttgc
     4681 gaaccacgca gggggcacta tggtagcgcc gcagaggcgg ggggtcgggg actgacatgg
     4741 cgtttctgga tcgcggcgcg cgctcggtgc tgctctggga gctggtccag ggcctgtggc
     4801 tcacgctcaa atacatgttc cagccgcctg tcacggtgaa ctatccctat gagaaggggc
     4861 cgctgtcgcc gcgctttcgc ggcgaacacg cgctccggcg ctatcccaat ggcgaggagc
     4921 gctgcatcgc ctgcaagctc tgcgaggcca tctgtcccgc gcttgccatc accatcgagg
     4981 ccgagccccg cgaggacggc agccggcgca cgacgcgcta cgacatcgac atgaccaaat
     5041 gcatctattg cgggctctgc caggaggctt gcccggtcga cgcgatcgtc gaagggccga
     5101 acttcgagtt cgccaccgag acgcgcgagg agcttttcta cgacaaggac aagctcctcg
     5161 ccaatggcga ccgctgggag gcggagctcg ccaagaatat cgcgctcgac gcgccctatc
     5221 gatgagcggc acatgatcat tcaggcgatc tgcttctggc tcttctcctt cgtgctggtg
     5281 ggatgctccg tcatggtggt gtccgcgcgc aatccggtcc attccgtgct gttcttgatt
     5341 ttggcgttct tcaatgcggc cgctctcttc ctgctgctcg gcgccgagtt cctcgccttg
     5401 atcctggtca tcgtctatgt cggcgccgtc gccgtgctgt tcctcttcgt ggtgatgatg
     5461 ctcgacatcg acttcgccga actcaggcgc gggttcatca cgtatctgcc gatcggcgca
     5521 ctcatcggat tgatgctgtt cctcgagctg ttcctggcct tcgccgcgtg gacggtggcg
     5581 cccgacgtcg cgaagagcat cgccgcgccg acgcccgcgc tttccggcct caccaacacc
     5641 caggcgctgg gcgcgctgat ctacacgcgc tatgtctatg ccttccagac cgcaggcatg
     5701 gtgctgctcg tcgccatggt gggcgcgatc gtgctgacgc tgcgcgcccg tgtcggggtg
     5761 cgccggcaga agattgcggt gcagctggcg cgccggcgcc aggagacgct cgccatcgtc
     5821 aaggtccagc cgcgccaggg gatttaggag cctgtctgag taatggaacg ggtctgcgac
     5881 attggcattt accgctgggc taggagcg
//
LOCUS       DASYNS010000245        12097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1774642, whole genome shotgun sequence.
ACCESSION   DASYNS010000245 DASYNS010000000
VERSION     DASYNS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 12097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12097
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774642"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1739)
                     /locus_tag="VGP50_07635"
     CDS             complement(177..1739)
                     /locus_tag="VGP50_07635"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEV8027287.1"
                     /translation="MNDTVMAEAPAATGTAATPPREPVLRVVRGSKIYGGVHAIEAVD
                     FDLYPGEVHAVVGENGAGKSTLCKAIAGAIQLTSGEYHVDGRPARFERPGDALRAGIA
                     MVYQETSLVPTMTAAQNIELGNEKLLTRFRSLNIQAQQLLQSLNFQVDPSLPVAFLGT
                     AKRQMVEIARAIYNEARVIIFDEPTASLTPEEIVHFFHLVRDLRSRGVAIIYISHALE
                     ESLQISDRVTVLRDGKLVVTAKTADMTREKLVRAMVGRDITQTHYVRRERAADKRGQP
                     REKVLAVENVTMGNVVKNMSFSVYAGEVVGIAGLIGSGRTEIAKIIYGALKRNLINGG
                     MIYLKGRPIRYRVPKQAINDGIVYITEDRKLNGYFETMVIDDNIHVGRLATRMGWRFL
                     LSRGERKKLADRWVERLKIGALQRKAKIAELSGGNQQKVVVAKSLVQDPSIVIFDEPT
                     RGVDVATIPEIHAAIRKLADEGKAVVVISSYLPEILSISDRILVAQRGRIVAEFNAAD
                     ATEDKIMYAAIH"
     gene            complement(1902..3011)
                     /locus_tag="VGP50_07640"
     CDS             complement(1902..3011)
                     /locus_tag="VGP50_07640"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEV8027288.1"
                     /translation="MSFLPHLMRDIRQIVFRDVDQHAIPAMDGALSPNDRLDACRPIG
                     APIPGADDVVAAPDGALYVSGDRQVLRLEGEGYERRSVFASFEGKAGGLALHPDGRLL
                     VCVAGRGLAAVDRGGRQSWLSQAADRPLGCLTGVAAGPDGTIFLSDGSARYVADDWCR
                     DLMEKNRLGRLVACAPGLEAPQVLLDQLAYPHGVALSRDGKQLWFTESWSHRVSRAPI
                     EGSGIGRPEIVIRNLPGYPARLGKAESGFWLSIFARRTHLIELVLREDEYRAEMTRTI
                     EPALWIAPALASAGHFLEPMQMGNIKALGIHKPWAPPRSYGLVALIDEQGDAIESLHS
                     RVGGRYHGITAACETKQGLVIVSKGHGRVLLEPRA"
     gene            complement(3008..5143)
                     /locus_tag="VGP50_07645"
     CDS             complement(3008..5143)
                     /locus_tag="VGP50_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEV8027289.1"
                     /translation="MSLTTWETFRGSVERLRYRWSLRLLLSELLQKQWFEPAIPFAVM
                     VGLWAYFASVLPNYASIASTESLARLYAEFGFVALGMAFSIIAGGIDLSVGAIFAFCN
                     FAALAYVNILELPIWLVVPATLATGAAVGAGNGFLIGYLKARPFLTTLVTLIIVRAVV
                     NILNEHYATVFATNAVDSDAWDFVATGSVLGIPINAAALLAVLFIGHIFLSRSRYGWH
                     IAALGASRKAARHAGIGVERMLFWTYVLSGALCGAAGLFYAARQGSTDSTTGFGWEFQ
                     AVTGVVLGGVLFRGTVWRAMIGALIIFLLNNGLVRMGVPGYVSSAAVGLILLAAVGID
                     VKWSRNRGKAIQKIYLNPTVVEFAPAPSLDRGSGSPYAENDGLLGAEAIGLNQVEGPE
                     DVILDRQGRLYGSTRDGNIIRFSGPGFAKREVFAHIGGRPYGMAFDKDENLIICIGGM
                     GLYGVRPDGQVFKLADETNRTWYKLNDDSRIRMADDLDIAPDGKIYFSDATTRYEATT
                     WALDGIEGRGNGRIVCHDPATGKTWTLLRHIPFPNGVCMAHDGRSFLFASTWLCKIYR
                     YWLDGPKKGKVEIFVDNLPGNPDNINRASDGTYWLALVGIRTPTFDLASRMPGFRLRM
                     VKQIPGDEWLSAGVNHGCVVRLSEDGQPIETLWDPSGAAHSTITSMREHQGYLYLGGL
                     ENNRIGRVKLKRGNPSWVSWESYWGSMRR"
     gene            complement(5369..6388)
                     /locus_tag="VGP50_07650"
     CDS             complement(5369..6388)
                     /locus_tag="VGP50_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV8027290.1"
                     /translation="MNRSIIGLIATMLVAVPAIAFAQEEGGTKTAHELRAAYDKDVKG
                     KTIAFLPIALGVPLSDEWAYVIKTQAEWRGMKFELRDPNANMAQMQQALTALVDEKPD
                     VLVVQNPSVTLLMKELKRAEGEGIHVIQMNMSSNYKSDVFVGADWFEIGRMLAEDVVK
                     ECGTGSGKSGKVQIVQGELTAAASVDQIAGVMDVLNKDKAINVVSNQAANWDANAAGN
                     ITATVIQQNPDLCASIGFWGIMQSGAAQAIRNAGKIDQVKVYASGEGSQLDCDQVSSG
                     NFYKFLSYTAGLQGQDVFDAALMVLQSGDKAGTKHHELYTRPIWLDKDNAKGANCFPL
                     PKVTG"
     gene            complement(6429..7454)
                     /locus_tag="VGP50_07655"
     CDS             complement(6429..7454)
                     /locus_tag="VGP50_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8027291.1"
                     /translation="MSSEASTPSFLRPQRGATRRRSFKLTQEQIVLLITLALLAVFSL
                     TLPGFATTANLLNLLRSVSILGILGLGLGVIVISRGIDLSEVAIMSGPWIVSLAEIQA
                     GVPVFWALALGLALALAIGFVNGVLVAFVEAPALFVTLATAYVVFGLALWYVGVLIAY
                     APQGPSDMMVLGQGKLLGVPMPIVVFAAAAIVVHLFLSQTSIGRFIYAQGDNTEAARL
                     SGVAIRPLIILEYLIVAALAWLAGWVWVGSNGSMSMVVTQQGTLIYDVILVVVLGGIS
                     LVGGRGSVLSVIAGCALIGTLLNGFTIMDVNSEVQNIIRGVVLLGAIVLDNRLHPRDE
                     ETARQGD"
     gene            7763..8140
                     /locus_tag="VGP50_07660"
     CDS             7763..8140
                     /locus_tag="VGP50_07660"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEV8027292.1"
                     /translation="MGQGDKDDALRNLQERLDKARRAAGLPTDGAPHSSGASVPGNAL
                     AIGFRIGIELVVAVFVGVGLGWAFDKWLGTKPWGFILFLFLGFGAGVANVFRVALGNQ
                     RAVGYAPRKSGTQEPVNWSDDED"
     gene            8142..8876
                     /locus_tag="VGP50_07665"
     CDS             8142..8876
                     /locus_tag="VGP50_07665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV8027293.1"
                     /translation="MASQLNPLKQFEIKPLIELPHIGGIDFSYTNSALFMTLAVVLAT
                     AFIVLSTRRAALVPGRWQSMAEFIYEFIANMIEENAGTEGMQYFPYIFTLFMFVLFGN
                     LLGMVPYAFTYTSHIIVTFAMAAVIFVAVTVIGFKRHGLHFFTLFAPKGVPWVVLFLL
                     VPIELISYFIRPFTLSIRLFANMLAGHAILAVFAGFVAALGIFGIGPLAMDVGLILLE
                     FLVAVLQAYIFTILTCLYLNDAIHLH"
     gene            8929..9156
                     /locus_tag="VGP50_07670"
     CDS             8929..9156
                     /locus_tag="VGP50_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV8027294.1"
                     /translation="MEIASAKLIGAGIACIALVGAGIGIGIIFAALINALARNPAAAA
                     QVTTPAYVGFALAEATGLFGFAVALILIFVL"
     gene            9167..9658
                     /locus_tag="VGP50_07675"
     CDS             9167..9658
                     /locus_tag="VGP50_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HEV8027295.1"
                     /translation="MPQLDFSTYPGQLFWLALSFIALYLLMSRLGLPRISGIIAARRQ
                     RVTADLEGAGQMKTQAEAVIAAYERALAEARAEAQRTLKETTERLNAQAAERLREIAQ
                     KLAGETAAAERRIAEAKAAALGNVRAVAVDVARAATAKLTGIAVDAERTAAAVDAALR
                     ERV"
     gene            9658..10152
                     /locus_tag="VGP50_07680"
     CDS             9658..10152
                     /locus_tag="VGP50_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV8027296.1"
                     /translation="MLAEIIHDPEFWVGVAFVIVVIGLAWKGGPVIAKSLDDRAVKIK
                     TEIAEAARLRDEAERMLADYQRKQRDALKEAELIIAQARREAEDAAAQGARDLAAALD
                     RRQRTALEKIALAESKATAEVRNAAVDVAIAAVRHILSEAIDPVRKSKLTDDAIADLP
                     HRLN"
     gene            10486..10707
                     /locus_tag="VGP50_07685"
     CDS             10486..10707
                     /locus_tag="VGP50_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEV8027297.1"
                     /translation="MQKIPGGWSWWYLLFIVQFVVALWPPFYNKAEPSFIGIPFFYWY
                     QLLWVLIGATFTALVYFMTERRHGGGKRQ"
     gene            10744..>12097
                     /locus_tag="VGP50_07690"
     CDS             10744..>12097
                     /locus_tag="VGP50_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV8027298.1"
                     /translation="MADVNWVAFIIFVLLFAVITWVGFAAAHWRKGDLDQLHEWGLGG
                     RRFGTVITWFLVGGDVYTAYTFIAVPALAFGAGAVAFFAVPYTIMIYPILFLAFPRLW
                     YVCHKHGYITAGDFVRGRYGNRWLALAVTITGIVATMPYIALQLVGIQVVIGALGISG
                     TGIWADLPLIIAFIILAAFTYSSGLRAPASIAIIKDILIYITAFAAVIVVPIELGGFG
                     KIFAAVPPAKLLLAVPGANTTGAYGNYATLALGSALALFLYPHSMTGILSASSGRAIR
                     RNAALLPGYSFMLGLLALVGFFAIAAGVKELPEFAAGFKQFGNNFAVPALLLHSFPSW
                     FVGIAFAAIGIGALVPAAIMSIAAANLYTRNIHREFINPNPTDKQESQMAKVVSLVVK
                     FGALVFILFVPTQYAIQLQLLGGVWIIQTMPSVMLGAYTRWFNDWALLVGWAAGTAAG
                     T"
BASE COUNT         1981 a         4130 c         3879 g         2107 t
ORIGIN      
        1 agccgccttg attctaaaat tgcgtaccag accgctcggc acgccatatc gaggcagcgc
       61 taccctcaat cgccctctcg accccgcgag tcgccgatgc cccagaaaca ccgccgacca
      121 aaagttaacg cctatgaggg ttggggtgag gggtgcggcg cccgggtgcg cgcgcctcag
      181 tgaatcgccg cgtacatgat cttgtcctcg gtggcgtcgg ccgcgttgaa ctcggcgacg
      241 atgcggcctc tctgtgccac gaggatgcga tcggagatgc tgaggatctc cggcaggtat
      301 gacgagatga cgaccaccgc cttgccttcg tcggcgagct tgcggatggc ggcgtggatc
      361 tccgggatcg tcgccacgtc gacgccgcgc gtcggctcgt cgaagatgac gatcgagggg
      421 tcctgcacca gcgatttcgc cacgacgacc ttctgctggt tgccgcccga gagctcggcg
      481 atcttcgcct tgcgctgcag cgccccgatc ttcagccgct cgacccagcg gtcggccagc
      541 ttcttgcgct cgccccgcga caggaggaag cgccacccca tccgggtggc gagccggccg
      601 acatggatgt tgtcgtcgat caccatcgtc tcgaaatacc cgttgagctt ccgatcctcg
      661 gtgatgtaga cgatgccgtc attgatcgcc tgcttgggca cgcgatagcg gatgggcctg
      721 cctttgagat agatcatccc gccattgatg aggttccgct tcaaggcgcc ataaatgatc
      781 ttggcgatct cggtccggcc cgagccgatg aggccggcaa tgccgacgac ctcgccggca
      841 tagaccgaga aggacatgtt cttgacgacg ttgcccatcg tcacattctc gaccgcgagc
      901 accttttcgc gcggctgacc gcgtttgtcg gcggcgcgct cacgccgcac gtaatgggtt
      961 tgcgtgatgt cgcgcccgac catggcccgc accagcttct cgcgcgtcat gtcggcggtc
     1021 ttcgccgtca ccaccagctt gccgtcgcgc agcaccgtga cgcggtcgga aatctgcagc
     1081 gattcctcga gcgcatgaga gatatagatg atggcgacgc cgcgactcct tagatcgcgc
     1141 accagatgga agaagtgcac gatctcctcg ggcgtgaggc tggccgtcgg ctcgtcgaag
     1201 atgatgacgc gggcctcgtt gtagatggcg cgcgcgatct ccaccatctg ccgcttggcg
     1261 gtgccgagaa acgccacggg caggctggga tcgacctgaa aattcagcga ttgcagcagt
     1321 tgctgcgcct ggatgttgag cgagcggaag cgcgtcagca gcttctcgtt gccgagctcg
     1381 atgttctgcg ccgccgtcat ggtcggcacc aggctcgtct cctgatagac catggcgatg
     1441 ccggcacgga gcgcatcgcc cgggcgctcg aagcgcgcgg gcctgccgtc gacgtgatat
     1501 tcgccggagg tgagctggat cgcgcccgcg atcgccttgc acaaggtcga cttcccagcg
     1561 ccgttctcgc ccaccacggc atgcacttcg ccgggataga gatcgaaatc caccgcctcg
     1621 atcgcgtgga cgccgccata gatcttgctg ccgcgcacca cgcgcagcac cggctcgcgc
     1681 gggggagtcg cggcggtgcc ggttgcagcc ggagcttcgg ccatcacggt atcgttcatg
     1741 cgcgcaccct cttacccctc accctcccag cgctgcgcgc tgggcccctc cctctcccgc
     1801 agggggagag ggattcttgc cgccgctcgg tgaactccct ctccccttgc gggagagggt
     1861 aggggtgagc ggccgccgcg cgctgcgcgt tcgggacgaa ctcatgcgcg cggctccagc
     1921 agcacccggc catgcccctt ggagacgatg accagcccct gcttcgtctc gcacgccgcg
     1981 gtgatgccgt gatagcgccc gccgacgcgg ctgtgcaggc tctcaatggc gtcgccctgc
     2041 tcgtcgatca gcgccacgag cccgtaagac cgcggcggcg cccacggctt gtggatgccc
     2101 agcgccttga tgttgcccat ctgcatcggc tcgaggaaat ggccggccga agcgagcgcc
     2161 ggcgcgatcc agagcgccgg ttcgatcgtc cgcgtcatct ccgcgcggta ctcgtcctcg
     2221 cgcaggacca gctcgatcag atgcgtgcgt cgcgcaaaga tgctgagcca gaagccgctc
     2281 tccgccttgc cgagacgcgc gggatagccc ggcagattgc ggatcacgat ctcgggccgg
     2341 ccgatgccgc ttccctcgat cggcgcgcgg ctcacgcgat ggctccagct ctcggtgaac
     2401 cagagctgct tgccgtcgcg cgagagtgcc acgccgtggg gataggcgag ctgatcgaga
     2461 agcacctgcg gcgcctcgag gccgggcgcg caggcgacga gccggcccag ccggttcttc
     2521 tccatgagat cccggcacca gtcgtcggcg acatacctcg cgctgccgtc gctcagaaag
     2581 atcgtgccgt ccggtcctgc cgccacgccg gtgagacagc ccagcggccg gtccgcggcc
     2641 tggctcagcc agctctgccg gcccccgcga tcgaccgcgg caaggccgcg cccggcgacg
     2701 cagacgagaa gccgtccgtc gggatgaaga gcgaggccgc ccgccttccc ctcgaagctg
     2761 gcgaagaccg agcggcgctc atagccctcg ccctcgagcc gcagcacctg gcgatcgccc
     2821 gagacataga gcgcgccgtc cggcgcggcg acgacgtcgt cggcgccggg aatgggcgcg
     2881 ccgatcggac ggcaggcgtc gaggcgatca ttggggctga gcgcgccgtc catcgcggga
     2941 atcgcgtgct gatcgacgtc gcgaaagacg atctggcgga tgtcgcgcat gagatggggg
     3001 agaaagctca tcggcgcatg ctgccccaat aggactccca gctcacccag ctcgggttgc
     3061 cgcgcttcag cttgacgcgc ccgatgcgat tgttctcgag cccgccgaga tagagataac
     3121 cctggtgctc gcgcatcgag gtgatcgtcg aatgcgccgc cccgctcggg tcccacagcg
     3181 tctcgatggg ctggccgtcc tcgctgagac ggaccacgca gccatgattg acgcccgccg
     3241 agagccattc gtcgccgggg atctgcttca ccatccgcag ccggaagccg ggcatgcgcg
     3301 aggcgagatc gaaggtcggc gtgcggatgc cgacgagcgc cagccaatag gtcccgtcgg
     3361 acgcgcggtt gatgttgtcg ggattgcccg ggaggttgtc gacgaagatc tcgaccttcc
     3421 ccttcttcgg cccgtcgagc cagtagcgat agatcttgca gagccaggtg ctggcgaaga
     3481 ggaaggacct cccgtcatgc gccatgcaga caccattggg gaagggaatg tggcgcagca
     3541 gcgtccaggt cttgcccgtc gcgggatcgt ggcagacgat gcggccattg ccgcgcccct
     3601 cgatgccgtc gagcgcccag gtcgtcgcct cgtagcgcgt cgtggcgtcg ctgaaataga
     3661 tcttgccgtc gggcgcgatg tcgaggtcat cggccatgcg gatgcgcgaa tcgtcgttga
     3721 gcttgtacca ggtgcggttg gtctcgtcgg cgagcttgaa tacctgcccg tcgggccgca
     3781 cgccgtaaag ccccatgccg ccgatgcaga tgatgaggtt ctcgtccttg tcgaaggcca
     3841 tgccataggg gcggccgccg atatgggcga agacctcgcg cttcgcgaag ccgggcccgg
     3901 agaagcggat gatgttgccg tcccgcgtgc tgccatagag ccggccctga cggtcaagaa
     3961 tgacgtcctc gggcccctcg acctggttga ggccgatcgc ttcggcgccg agcagcccgt
     4021 cattctccgc gtaaggcgag ccgctgccgc gatcgaggga cggcgccggc gcgaactcca
     4081 ccaccgtcgg gttgagatag atcttctgga tcgccttgcc gcggttcctc gaccatttga
     4141 cgtcgatgcc gacggccgcc agcaggatga ggccgaccgc cgccgagctg acatagccgg
     4201 gcacgcccat gcgcacgagg ccgttgttca aaaggaagat gatgagcgcg ccgatcatcg
     4261 cccgccacac cgtgccgcgg aacaggacgc cgcccagcac gacgccggtc acggcctgga
     4321 actcccagcc gaagccggtc gtcgaatcgg tgctgccctg gcgggcggca tagaacaatc
     4381 cggccgcccc gcagagcgct cccgacagca cataggtcca gaacagcatc cgctcgacgc
     4441 cgatgccggc atgacgcgcc gccttgcggc tcgcgccgag cgcggcgatg tgccagccgt
     4501 agcgggagcg gctcaagaag atgtggccga tgaacagcac cgcgagcagc gccgccgcgt
     4561 tgatcgggat gcccagcacc gagccggtgg caacgaagtc ccaggcgtcg ctgtcgacgg
     4621 cattggtcgc gaagaccgtg gcgtagtgct cgttgagaat attgacgacc gcgcggacga
     4681 tgatcagcgt caccagcgtg gtgagaaacg gccgcgcctt tagatagccg atgaggaagc
     4741 cgttgccggc gcccaccgcc gcgccggtcg ccagcgtcgc cggcacgacg agccagatcg
     4801 gcagctcgag aatgttgaca taagcgagcg ctgcgaaatt gcagaacgcg aagatcgcgc
     4861 cgacgctcag atcgatgccg ccggcgatga tcgagaaggc catgcccagc gcgacgaagc
     4921 cgaactccgc atagagccgc gccagcgact cggtgctggc gatgctcgca tagttcggca
     4981 gcacggaagc gaaataggcc cagagcccga ccatcaccgc aaagggaatg gccggctcga
     5041 accattgctt ctgcagcagc tccgacagca ggagcctgag cgaccagcga tagcgcagcc
     5101 gctcgacgga ccctctgaac gtttcccagg tggtaagcga cattgcgctc cctctaccgg
     5161 cttgcgcgct tccacccctc tcccgcattg cgggagaggg tggcgcgcgc ggagcgcgcg
     5221 ccgggtgagg gggcgtcaac ggacgaagct cgcttgcgcc ctcccctcgc cctcccggcg
     5281 cttcgcgccg ggcccctccc tctcccgcat tgcgggagag gggcaagttg ctgcggcgtg
     5341 gtcacacgca actgtccacg agcacgcgct agcccgtcac cttcggcagc gggaagcaat
     5401 tcgcgccttt ggcattgtcc ttgtcgagcc agatcgggcg ggtatagagc tcgtggtgct
     5461 tcgttcccgc cttgtcgccg ctttgcagga ccatgagcgc cgcgtcgaac acgtcctggc
     5521 cttggaggcc ggcggtgtag ctcaggaact tgtagaagtt gcccgagctc acctggtcgc
     5581 aatcgagctg cgagccttcg cccgaggcat agaccttgac ctgatcgatc ttgccggcgt
     5641 tgcggatcgc ctgggcggcg cccgactgca tgatgcccca gaagccgatg ctggcgcaga
     5701 ggtcgggatt ctgctggatg accgtcgcgg tgatgttgcc cgcggcattg gcgtcccagt
     5761 tggcggcctg gttcgagacc acgttgatgg ccttgtcctt gttgaggacg tccatgaccc
     5821 cggcgatctg gtcgacgctg gcggcggcgg tgagctcgcc ctgcacgatc tgcaccttgc
     5881 cggacttgcc gctgccggtg ccgcactcct tcacgacgtc ctcggcgagc atgcggccga
     5941 tctcgaacca gtcggcgccc acgaacacgt ccgatttgta gttggaggac atgttcatct
     6001 ggatgacatg gatgccctcg ccctccgcgc gcttcagctc cttcatcagc agcgtcacgc
     6061 tcggattctg caccaccagc acgtcgggct tctcgtcgac cagcgccgtc agcgcctgct
     6121 gcatctgcgc catgttggca ttgggatcgc gcagctcgaa cttcatgccg cgccattccg
     6181 cctgcgtctt gatgacatag gcccattcgt cggacagcgg cacgcccagc gcgatcggca
     6241 ggaaggcgat ggtcttgccc ttgacgtcct tgtcataggc ggcgcgcagc tcatgcgcgg
     6301 tcttggtccc gccttcctct tgcgcgaagg cgatggccgg caccgcgacg agcatcgtcg
     6361 cgatgaggcc gatgatcgat ctgttcatgt tgatgtcctc cctctttact cttcgtgaat
     6421 cggtggcgtc aatcgccttg gcgcgccgtc tcctcgtcgc gcggatggag ccggttgtcg
     6481 agcacgatcg cgcccagcag gacgacgccg cggatgatgt tctgcacctc gctgttgacg
     6541 tccatgatgg tgaagccgtt caagagcgtg ccgatgaggg cgcagccggc gatcaccgac
     6601 agcacgctgc cgcgtccgcc gacgaggctg atgccgccca gcacgaccac gaggatcacg
     6661 tcgtagatca gcgtcccctg ctgggtcacg accatgctca tgctgccgtt cgagccgacc
     6721 cacacccagc cggcgagcca ggccaacgcc gccacgatca gatattcgag gatgatcagc
     6781 ggccggattg ccacgcccga aagccgcgcc gcctcggtat tgtcgccttg ggcataaatg
     6841 aagcgtccga tcgaggtctg cgacaggaac aggtgaacga cgatcgccgc ggcggcgaag
     6901 accacgatcg gcatcggcac gccgaggagc ttgccctgcc ccagcaccat catgtcgctc
     6961 ggtccttgcg gcgcataggc gatcagcacg ccgacgtacc agagtgcgag cccgaacacg
     7021 acataggccg tcgccagcgt gacgaacaag gccggcgctt cgacgaaggc cacgagcacg
     7081 ccattgacga agccgatcgc cagcgcgagc gccaggccca gcgccagcgc ccagaacacc
     7141 ggcacgcccg cctggatctc ggcgagagag acgatccacg ggcccgacat gatcgcgacc
     7201 tcgctgaggt cgatgccgcg gctgatgacg atgacgccga gaccgagacc gagaatcccg
     7261 aggatcgaga cgctgcgcag caggttgagc aggttggcgg tggtggcgaa gccgggcagc
     7321 gtcaggctga acaccgccag aagcgccagc gtgatgagaa ggacgatctg ctcttgcgtg
     7381 agcttgaaag agcgccggcg tgtcgcgccc cgttgcggcc gcaggaacga gggcgtcgaa
     7441 gcttcgctgc tcatcgccgc cgctgccgcg cgcggcgggc gctaccggcc gttgcgcgcg
     7501 gagtccaccc tgcaaccacc ttttcgatcc tcccggtgcg cctctctgta cgatggaggc
     7561 ttttcgatga ccaaaactat accggcatct gcgcgagcgt caacaatcag cgactcaacg
     7621 tctccgagta ttgtccgaca aatgccgatg ctgcgggcgt tcttgacact cccgccatgc
     7681 gcccctatgg ttcgcgccgt tttggcgccg aggcggccgg gatcttgagg gggagtcgca
     7741 acgcgtcgcg ggtggttggc cgatgggcca aggcgacaaa gacgatgcac tgcgaaacct
     7801 ccaggagcgc ctcgacaagg cgcgccgcgc ggcgggtctg ccgacggacg gcgcgcccca
     7861 ttcgagcggc gcttccgtcc ccggcaacgc gctggcgatc ggatttcgca tcggcatcga
     7921 gctggtggtc gccgtgttcg tgggcgtcgg tcttggctgg gccttcgaca agtggctcgg
     7981 caccaagccc tggggattca tccttttcct gttcctcggg tttggcgcgg gcgtcgcgaa
     8041 tgtcttccgc gtcgcgcttg gcaatcagcg ggcagtcggc tacgccccgc gcaagagcgg
     8101 cacgcaggag ccggtgaatt ggtcggacga cgaggattga cgtggcgtcg caactcaacc
     8161 cgctgaagca gttcgagata aagccgctga tcgaacttcc ccatatcggc ggaatcgact
     8221 tctcctacac caactccgcg ctcttcatga cgctcgccgt cgtgctggcg acggcgttta
     8281 tcgtgctcag cacgcgccgc gccgcgctcg tgccggggcg ctggcaatcc atggcggagt
     8341 tcatctacga gttcatcgcc aacatgatcg aggagaatgc cggcaccgag gggatgcagt
     8401 acttccccta catcttcacc ctcttcatgt tcgtgctctt cggcaatctc ctgggcatgg
     8461 tcccctacgc cttcacctac acgagccaca tcatcgtcac cttcgccatg gcggcggtga
     8521 tcttcgtcgc cgtgacggtg atcgggttca agcgccacgg cttgcacttc ttcacgctct
     8581 tcgcgcccaa aggggtgccg tgggtcgtgt tgttcctgct ggtgccgatc gagctcatct
     8641 cctatttcat tcgccccttc acgctgtcga tccggctctt cgcgaacatg ctcgcgggtc
     8701 acgccattct cgccgtgttc gcgggcttcg tggccgcgct gggcattttc ggcatcggcc
     8761 cgctcgccat ggatgtcggc ctgatcttgc tggagttcct ggtcgccgtc cttcaagcct
     8821 acatcttcac cattctcacg tgtctctatc tgaacgacgc catccacctg cactgactct
     8881 cgaccaacca acccgccctc cccaactcaa cctccgagga ccaagcaaat ggaaatcgca
     8941 tcggccaagc tgatcggcgc cggcatcgcc tgcatcgcgc tcgtcggcgc cggcatcggc
     9001 atcggcatca tcttcgcggc cctcatcaac gcgctcgccc gcaaccccgc cgcggcagcg
     9061 caagtgacga cgccggccta tgtcggtttc gcgctcgccg aagcgacggg cctcttcggc
     9121 ttcgccgtgg ccttgatcct catcttcgtg ctctgaccag gggccaatgc cgcagctcga
     9181 cttctccacc tatccgggcc agctcttctg gctggcgctc agcttcatcg cgctctacct
     9241 gctgatgtcg cggctcggct tgccgcgcat cagcgggatc atcgccgcgc ggcggcagcg
     9301 cgtgaccgcc gatctggagg gggccgggca gatgaagacc caggcggagg ccgtgatcgc
     9361 ggcctatgag cgggcgctgg ccgaggcgcg tgccgaggcg caacgcaccc tcaaggagac
     9421 caccgagcgc ctcaacgccc aagccgccga gcgcctgcgc gagatcgcgc agaagctcgc
     9481 gggcgagacg gcggcggccg agcggcggat cgccgaggcc aaggcggcgg cgctcggcaa
     9541 tgtgcgggcc gtcgccgtcg acgtggcgcg ggccgccacg gcaaagctca ccgggatcgc
     9601 ggtcgatgcg gagcgcaccg ccgcggcggt cgacgcggcg ctgagggagc gggtctgatg
     9661 ctggcggaga tcatccacga cccggaattc tgggtcggcg tggccttcgt catcgtcgtc
     9721 atcggcctcg cctggaaggg cgggcccgtg atcgccaagt cgctcgacga ccgcgcggtc
     9781 aagatcaaga ccgagatcgc agaagcggca cgcctgcgcg acgaggcgga gcgcatgctc
     9841 gccgactacc agaggaagca gcgcgacgcc ctcaaagagg ccgagctgat catcgcccag
     9901 gcgcgccgcg aggcggaaga cgccgccgcc caaggggcgc gcgacctcgc cgccgcgctc
     9961 gaccgccgcc agcgcacggc cctcgagaag atcgcgcttg ccgagagcaa ggcaacggcc
    10021 gaggttcgca acgccgccgt cgacgtggcg atcgccgccg tacgccacat cctttccgag
    10081 gcgatcgacc ccgtgcgcaa gagcaagctc accgacgacg ccatcgccga cctgccgcac
    10141 cgcctcaact gaagcgcgcc gaacatctcc ctctcccgca ttgcgggaga gggagggacc
    10201 caacgcgaag cgttgggagg gtgagggcgc gcctcaggca aagcaccctt acttgatgcg
    10261 cccccacccg gcgcgggctc cgcccgcacc cccctctccc gcgatgcggg agaggggcaa
    10321 ggacgcgcct tcacgcggga gtatatttgt cgcaagcgcg aacgctcgca cgatgccgac
    10381 tcgcggcatt gccgatgctg gcgggcgatg aagcgcgcgt gtatcatttc gcccgaaaac
    10441 tgccggagaa ccggcgcctg atcgggggag atcgccgtga acaacatgca aaagattccg
    10501 gggggatgga gctggtggta cctgctcttc atcgtccaat tcgtcgtcgc cctctggccg
    10561 cccttctaca acaaggccga gccctctttc atcggcattc ccttcttcta ctggtatcag
    10621 ctcctgtggg tgctcatcgg cgccacgttc actgccctcg tctatttcat gacggagcgc
    10681 cggcacggcg gcggcaagcg ccagtagcgg aaacaggggg aaacgaatcg gggggaggac
    10741 gtcgtggcag acgtaaattg ggtcgcattc atcatcttcg ttctgttgtt cgccgtcatc
    10801 acctgggtcg gcttcgccgc cgcccattgg cgcaagggcg atctcgacca gctgcatgaa
    10861 tgggggctcg gcggccggcg tttcggcacg gtcatcacct ggttcctggt cggtggcgat
    10921 gtctacacgg cctatacctt catcgccgtt ccggcccttg ccttcggcgc cggcgcggtc
    10981 gccttcttcg ccgtgcccta caccatcatg atctatccga tcctgttcct ggcgttcccc
    11041 aggctctggt atgtctgcca caagcacggc tacatcacgg cgggcgactt cgtgcgcggc
    11101 cgctacggca accgctggct cgcgctggcc gtgacgatca ccgggatcgt cgccaccatg
    11161 ccctatatcg cgctccagct cgtgggcatc caggtggtga tcggcgcgct cggcatttcc
    11221 gggacgggaa tctgggccga tctgccgttg atcatcgcct tcatcatcct cgctgccttc
    11281 acctattcga gcggcctgcg cgctccggcc tcgatcgcca tcatcaagga tatcctgatc
    11341 tacatcaccg ccttcgcggc ggtcatcgtc gtgccgatcg agctcggcgg cttcggcaag
    11401 atcttcgcgg ccgtgccgcc ggcgaagctg ctgctcgcgg tgcccggggc caacaccacc
    11461 ggcgcctatg gcaactatgc aaccctggct ttgggctccg ctctggcgct cttcctctat
    11521 ccccattcca tgacgggcat cctgagcgcc agcagcggcc gcgccattcg ccgcaacgcg
    11581 gcgctgctgc cgggctattc cttcatgctc ggccttctgg cgctggtcgg cttcttcgcc
    11641 atcgccgccg gcgtcaagga gctccccgaa tttgccgcgg gcttcaagca gttcggcaac
    11701 aacttcgcgg tgccggcgct gctgctccac agcttcccgt catggttcgt cggcatcgcc
    11761 ttcgccgcca tcggcatcgg cgcgctggtg ccggccgcga tcatgtcgat cgccgccgcc
    11821 aatctctata cgcgcaacat ccaccgcgaa ttcatcaacc ccaacccgac cgacaagcag
    11881 gaatcgcaga tggcgaaggt ggtgtcgctg gtcgtcaaat tcggcgccct cgtcttcatc
    11941 ctcttcgtgc cgacgcaata cgccatccag cttcagcttc tgggtggcgt ctggatcatc
    12001 cagacgatgc cctcggtcat gctcggcgcc tatacgcgct ggttcaacga ctgggcgctg
    12061 ctggtgggat gggccgccgg gacggccgcc gggacgg
//
LOCUS       DASYNS010000246         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2398749, whole genome shotgun sequence.
ACCESSION   DASYNS010000246 DASYNS010000000
VERSION     DASYNS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2398749"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /gene="cysQ"
                     /locus_tag="VGP50_07695"
     CDS             complement(<1..682)
                     /gene="cysQ"
                     /locus_tag="VGP50_07695"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEV8027299.1"
                     /translation="MSVDYRGLLELADAAAMEAGAAILRVYAGDFTVEQKADASPVTL
                     ADQLAEAVVLAKLGAATPEVPVIAEERAAKHGLPGAAPPLFWLVDPLDGTKEFIKRNG
                     EFTVNIALVERGRTVLGVVHVPAQGVTYAASGAGTATRRRGNGPAEPIAARAAPVRRP
                     ILVHSRSHADEQRIAQYVAALPEPECRAAGSSVKFCLIAAGEADLYPRFGPTMEWDTG
                     AAQAVLEAA"
     gene            complement(772..1521)
                     /locus_tag="VGP50_07700"
     CDS             complement(772..1521)
                     /locus_tag="VGP50_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEV8027300.1"
                     /translation="MLSFFRPALAVLLLFAVALALPARADEPLSAEQKEAVEQVIHDY
                     LMKNPQFLIEVLKAAQEKAKEAKADDARQAIAAKHDELSRDPGSPVGGNPKGDVTIVE
                     FFDYSCPYCKQVEPQIEALLKEDPRIRIVYKEFPVLGEGSVYASSMALAAVKQGKYAA
                     FHDAMIATKGHATEEMVLKVAAGVGIDVAKAKTTMNAPEIQDILKRNYALAESLEING
                     TPAFVIGNVLVPGATTVEHLKQLVADARKQG"
     gene            complement(1735..>2146)
                     /locus_tag="VGP50_07705"
     CDS             complement(1735..>2146)
                     /locus_tag="VGP50_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8027301.1"
                     /translation="LKGQMLFENGRIGEAVAPYEEAVRLDPQSALLRVEVAQVQLETN
                     DDALVPKALANLNEAVRFEDRNPEAWRFLAIAHGRSNNMGMMALALAEEGIADGNYGE
                     ARQQAARALKLLPAGPAKQRALDIQEEAKREGKP"
BASE COUNT          317 a          716 c          733 g          380 t
ORIGIN      
        1 cggccgcttc gagcaccgct tgggccgcgc ccgtgtccca ctccatcgtc ggcccgaacc
       61 gcggatagag atcggcctcg cccgcggcga tgagacagaa tttgacggag cttccggcgg
      121 cgcggcattc gggctcgggg agcgccgcga catattgcgc gatgcgctgc tcgtcggcat
      181 gcgagcggct gtggacgagg atgggacggc gcaccggcgc cgcgcgcgcg gcgatgggct
      241 cggccggtcc gttgccgcgt cggcgcgtcg cggtgccggc gccggaagcg gcgtaggtca
      301 cgccttgcgc cggcacatgc accacgccga gcacggtacg gccgcgctcg acgagcgcga
      361 tgttgacggt gaactcgccg ttgcgcttga tgaactcctt cgtgccgtcg agcggatcga
      421 cgagccagaa aagcggcggc gccgcgccgg ggaggccgtg tttcgcggcg cgctcctcgg
      481 caatgacggg aacctccggc gtcgccgcgc cgagcttcgc cagcacgacg gcttccgcga
      541 gctggtcggc gagcgtcacc ggcgatgcgt cggctttctg ctcgactgtg aagtcgccgg
      601 catagacgcg caggatcgca gcgccggctt ccatcgccgc ggcatcggca agctcgagca
      661 aaccgcgata atcaacgctc atctttgtct ttcgcgcggc ggcgcccgct tcgaggatga
      721 cggcgaactc attgagccgc agggcccgag atctgccgga gcggctgacg ctcagccttg
      781 cttgcgcgcg tcggcgacca gctgcttcag atgctcgacc gtggtggcgc cgggcacgag
      841 cacgttgccg atgacgaaag ccggcgtgcc gttgatctcg agggactccg ccagcgcgta
      901 attgcgcttc aagatgtcct ggatctcggg cgcgttcatc gtcgtctttg ccttggcgac
      961 gtcgatgccg acaccggcgg cgaccttcag caccatctct tcggtcgcgt gtcccttggt
     1021 cgcgatcatg gcgtcatgga aggcggcgta tttgccctgc ttcaccgcgg cgagcgccat
     1081 gctcgaggcg tagacggagc cctcgccgag cacgggaaat tccttgtaga cgatgcggat
     1141 cctgggatcc tccttgagca gcgcctcgat ctgcggctcg acctgcttgc agtaggggca
     1201 gctgtaatcg aagaactcga caatcgtcac gtcgcccttg ggattgccgc cgaccggcga
     1261 gcccggatcg cgagagagct cgtcatgctt cgccgcgatg gcctggcgcg catcgtccgc
     1321 cttcgcctcc ttggctttct cctgcgccgc cttcaacact tcgatgagga attgcgggtt
     1381 cttcatcagg tagtcgtgaa tgacctgctc gaccgcctct ttctgctccg ccgagagcgg
     1441 ctcgtcggcg cgggccggca gcgccagggc gacggcgaac agcagcagga cggcaagggc
     1501 ggggcggaaa aaggaaagca tgaacggggt ccctcgggtg ctgtggcgac gcgcgatcat
     1561 gcgtcagctt gcggccgagc acaactgtta cgcactccgg gcgggaatcg ccaattcggg
     1621 gcaggggcac ccctaatttg ccatccaagg agttcgcgcc tgctcggtgc ggctcccgcg
     1681 attgcaagag caatcgctgc cgccgccagc cttagacgag cgctacgcgc ccgtctaagg
     1741 cttgccttcg cgcttcgctt cttcctggat gtcgagcgcg cgctgcttgg cggggccggc
     1801 cgggagcagc ttgagggcgc gcgccgcctg ctggcgcgcc tcgccatagt tgccgtcggc
     1861 gatgccttcc tcggcgagcg ccagggccat catccccatg ttgttgctcc ggccatgggc
     1921 aatggcgagg aagcgccagg cctcgggatt gcggtcctcg aagcgcacgg cctcgttgag
     1981 attggcgagc gccttcggca cgagcgcgtc gtcattcgtc tcgagctgga cctgcgcgac
     2041 ctcgacgcgc aagagcgccg attgcgggtc gagcctgacc gcttcctcat aaggtgcgac
     2101 cgcctcgccg atgcggccat tctcgaagag catctgcccc ttgagc
//
LOCUS       DASYNS010000247        15386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4737439, whole genome shotgun sequence.
ACCESSION   DASYNS010000247 DASYNS010000000
VERSION     DASYNS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 15386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15386
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4737439"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VGP50_07710"
     CDS             complement(<1..732)
                     /locus_tag="VGP50_07710"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV8027302.1"
                     /translation="MRHAIIPLLALLVAACSSVGPETATVEDLSIPSMTPAVGRDWPD
                     KPLFVAGKLTVASQPLNLPGELYLPKNAPTPMPAVVLVHGSGGLDYEGTHIREWAGRL
                     NAWGVAALVIDTYGPRGITETVSDQQQISIFAQVADAYAALKQLAADPRIDRDHIAIM
                     GFSRGAIVTVDTAFETLRQGFGGGNLHFAAHVALYSGCNVAFADHATDKAPMLFLHGE
                     GDDFTPMAPCKEYATWLQSMGNAVTF"
     gene            944..1918
                     /locus_tag="VGP50_07715"
     CDS             944..1918
                     /locus_tag="VGP50_07715"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEV8027303.1"
                     /translation="MAKKPDWEIPILGQPKPEDFAFDLDRALLSVVGLQANIPEDAST
                     ASILGTERSGSGVVIRESGLVLTIGYLIIEAESLWLSTADGRAIPGHVLAHDWETGFG
                     LVQALGRLGVPALPLGRSSELAANDPVILAGGGRRQSVRASVIGKQEYAGYWEYLLEE
                     AIFTAPAHPFWGGTGLIGRDGTLLGIGSLNVQQVTDRGAPRKINMVVPIDVLKPVLDE
                     LLTYGRRNRAARPWLGLYSAESDGKVVIAALTERGPAAAAGMRVGDVVWAVRDASVES
                     LADFYRKVWASGAAGAEIPIEVLRDDRTVWVRVKSVDRQALLKSPRLQ"
     gene            complement(1943..3505)
                     /locus_tag="VGP50_07720"
     CDS             complement(1943..3505)
                     /locus_tag="VGP50_07720"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEV8027304.1"
                     /translation="MSLRGHLAGGAALGAAALLLIRPAAAQTSADQIEQLQQQIQQLR
                     EQSQQQIDRLQEQLQDLQSQMQKNQAFPAQAPAASAPPAAAAPTQAAAPPSTTVPGEP
                     RLTLYPAKGPAFTTADGQTSIQLASRVQFDLGDYFNVKPQSKFSSPSDLTSGVNARRA
                     RLGIVGKFYGDWDYALIYDFGGSADSLVNSGANTSGIENAYITYTGFRPLAIDAGYQN
                     VPWTLDEATSSTDILFLERASSAAVAFNIAGGDFRSAVDVRYINDRYFAGLWATGPVS
                     GQPHNVGEQFGALGRFSVQALQSSAGSLHLGVDVESLLKAQNVNGIPTLTLSDRPELR
                     IDPTVVLNTGSLLEVTNAEVYGAELAAAYENLFFQSEYYHYIIERNALAKLNFDGGYA
                     EASWVLTGEPHVYIPATGAYAGILPDHPMSFTGDGWGAFEVAARYSVVDLNDRFTPGV
                     AAATTQGVAGGKQTVYTLGLNWYVDTYVRLSLNYLHGIIDKQSGSVTTGPLGIGTGLK
                     FDAVAARWQVAF"
     gene            3834..4277
                     /locus_tag="VGP50_07725"
     CDS             3834..4277
                     /locus_tag="VGP50_07725"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV8027305.1"
                     /translation="MSEHVSVTITRQDKYRFLVDFGPGIEKAVTDEPAPLGDGAGPSP
                     QQLLAAAMANCLSASLVFAIAKFKGDPGPLTTTALCELGRNERNRLRVTAISVTMTLG
                     TAPESLEHLDRALAQFEDFCTVSQSVRAGIPFSVTVKGPDGRLLK"
     gene            complement(4286..4846)
                     /locus_tag="VGP50_07730"
     CDS             complement(4286..4846)
                     /locus_tag="VGP50_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027306.1"
                     /translation="MKAKIGTASMVVAAFALAVTLADAPSAAAFGGGGGGGHGGGGGG
                     RGSWGGHGGWGGRGFRGGFHDGRFHNGFHRRFFGGFAFGGFYDPLWWGYPAYAYGYPY
                     PYYGYPAYAYPAPAYAYPAPGEDDDSAPMAEAPPAAPPPAAAAPDDTGAPPAGYWYFC
                     QSAQAYYPQVSSCPEPWLQVPPRPTP"
     gene            complement(5257..5976)
                     /locus_tag="VGP50_07735"
     CDS             complement(5257..5976)
                     /locus_tag="VGP50_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HEV8027307.1"
                     /translation="MSFSHLHYLPLALPFFSMLVAVFLVLVALLQIGVLRYAYLRLGI
                     SPGAAMLLLLGSLIGSYFNIPVAELRGHQLAAGREITFYGMRYVVPVLVDSPGTVIAV
                     NVGGALIPGAMSLYLLARHRLWVRGAMATACVAVMCHVLADPVPGLGIALPVFVPALA
                     AAVVALLLSPRRAAPLAYIGGSLGTLIGADLLNLDKIQGLGAPVASIGGAGTFDGIFL
                     AGVLAVLIASLTGSRSAVAAR"
     gene            6132..6692
                     /locus_tag="VGP50_07740"
     CDS             6132..6692
                     /locus_tag="VGP50_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEV8027308.1"
                     /translation="MLVLGASSCVAPPYAEVTDCADFVQTPSEYHMEIAGRAGMLTQQ
                     EHSFVCYAGVLGRAQVTSAMMAQQRGVSPAVTRFAAITAGDQEMLNRRLAATAEQHAG
                     LIPPDGLDAPHLAMLDQLSQLSGEAFDRAYIEDQLQEAQATIAVLQEEMAEGSEPTLR
                     SFARDVLPYVEERAREAQSIIEQLPG"
     gene            7221..8507
                     /locus_tag="VGP50_07745"
     CDS             7221..8507
                     /locus_tag="VGP50_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8027309.1"
                     /translation="MKGLARLLTPHLHYAWVVAGVAFLTLLAAVAVRATPSVLIVPLE
                     QAFGWSSGTISFAIAINIVLYGLMGPFSGALMQRFGIRRTVLVALTLLAASVGASSFM
                     TQPWQLVLSWGIAVGLGSGATALVLGATIVNRWFAERQGVVMGFITASTATGQLVFLP
                     MLASVADKAGWRPVVWIAAAVAAAMIPIVALLLPDSPADIGLKRYGGEEESDAAVTTR
                     TNPLGIALSALGRASRSGGFWLLFASFFICGASTNGLIGTHLISYCIDHGMPAVEGAT
                     LLAAMGICDIIGTTGSGWLSDRYDSRWLLFWYYGLRGLSLLYLPYSGFSLSGLSIFAV
                     FYGLDWLATVPPTVRLTRDLFGKTEAPIVFGWLLTGHQLGAGLTAFEAGTLRAALGSY
                     LVPVLISGALCIIAALLVLRVGRNRSTLEPAAGTAF"
     gene            complement(8525..9412)
                     /locus_tag="VGP50_07750"
     CDS             complement(8525..9412)
                     /locus_tag="VGP50_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8027310.1"
                     /translation="MSDDIKQVRAFLGALPPLDSLPLAERRTRYDRAKHLFPLPAGVA
                     VARVDAGGVAAECIAMPASRAGRVVLYLHGGGYVMGSADSHRHLAAAIAEAAQASVLL
                     PAYRLAPEHPFPAALDDALAAYRGLLDGGQAPTSLVIAGDSAGGGLAVATLLAARDRG
                     LPLPAGAALISPWVDLSCSLVSLETLAARDPIVKKDSLAECARHYLVGGDLRNPLASP
                     LYGDLRGLPPLLIQVGSEEVLLDDARELDRRAREQGVRTTLEIWPEMIHVWHWFAAQL
                     REGRDAIARLGEFVRMMTG"
     gene            9597..10133
                     /locus_tag="VGP50_07755"
     CDS             9597..10133
                     /locus_tag="VGP50_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV8027311.1"
                     /translation="MTPRAIAIDWPADIHAALSAADIRQVASVPDAGHARLMTLCSAD
                     PRMRMVPLTSEQEGIGLAAGAWLGGQRSVLLMQSSGVGNCINALALIESCRFPFLTLI
                     TMRGEFGEFNPWQVPMGHAAGPSLELANIIVHRVSGAGEAGATVAGAARLAFASYRGV
                     AVLLSQQLIGAKSFGQQR"
     gene            10139..10723
                     /locus_tag="VGP50_07760"
     CDS             10139..10723
                     /locus_tag="VGP50_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV8027312.1"
                     /translation="MALDRRAVAGALLAQRGDLLVVAGLGAPAWDATAAGDHPLNFPL
                     WGAMGSAAMVGLGVALAQPSRPVLVLTGDGEMLMGLGSLATIAVQRPPNLAIVVLDNE
                     RYGETGMQPTHTAHGVDLAAVAAACGFAASTTIRTMTEVAALGATLRDGRGPRFAQVK
                     IAADNPPLVLPPRDGGLLKDRFRAALLGPDAALI"
     gene            complement(10724..11656)
                     /locus_tag="VGP50_07765"
     CDS             complement(10724..11656)
                     /locus_tag="VGP50_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEV8027313.1"
                     /translation="MAVFVLDPISPRALARLGSGVVDWRDPAIALWPEKADAVITRTS
                     PVGAADIERARHLKIIGKHGVGVDNIAVAAARARGIPVVNTPGANGDAVAELVLGLAL
                     AAARHIVTGDRALRLGNLAAAALPNGRELAGRRLGIVGFGQVGRRVAALFARALGMSV
                     AAYDAAVPGETIRAGGAAPAASFEALLRESDLLSLHVPLTRATLGLIGARELALMTEG
                     SILVNTARGGVVDEAALADALRRGRPAVAASDVFALEPPPPDHPLLELPNFIATPHLG
                     AATEEALERVGFLVVDQVLEVLAGRPARHPVIPQ"
     gene            11913..12524
                     /locus_tag="VGP50_07770"
     CDS             11913..12524
                     /locus_tag="VGP50_07770"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8027314.1"
                     /translation="MTAIPLPPSAVDWRRVEHWTAEIAGGSLRALRKTFGQGRGGPSI
                     LWRPPLSRLAAPQLHFLMGYWNGLAGARTMPEAREIDALEMRPALGYVSLLEVIEDGH
                     DFRYRVFGSAIAAVVGFDMTGRPASALKARAEVAEFALAIYRAVLARGEPLLAEHGPP
                     STISSVAWHSLVLPLADPGGAVVRLLAGAVPVARDGRALLPRL"
     gene            12667..13350
                     /locus_tag="VGP50_07775"
     CDS             12667..13350
                     /locus_tag="VGP50_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027315.1"
                     /translation="MTAFSTLSRLTRIGAFGIACSVIVACAQGNAETNSEPMAMHHAM
                     TAASSKAADLRSALDELLSEHVILAAEATNAALAGRQPDFQAAAAALDQNSIAISKAV
                     GSVYGAEAEAQFLPLWRSHINLVVDYTVGTASNDPAKAQQAVNKLLAYSGDLAGFFSG
                     ANPNLSKEAVADMVKEHIVTLKAVIDAQAKKDYAAEYTAQRTAYHHMGMMALALADGI
                     AKQFPQKFS"
     gene            13421..13981
                     /locus_tag="VGP50_07780"
     CDS             13421..13981
                     /locus_tag="VGP50_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV8027316.1"
                     /translation="MVMDNEIVALSSLEALVEAVARGSQPALRRIYEQESRRLYGVAL
                     RIARRPALAADALQDAFVQVWQKAGSFAAERGSAEAWLTGIVRYRALDAVRKTSREEP
                     SDDPALGDRAEDPDQIEQIDRLLSRDALRRCLGALDENQRRSILLAFVDGLSHAEISK
                     RLPAPLGSVKSWVRRGLLSLRSCLDS"
     gene            13978..14652
                     /locus_tag="VGP50_07785"
     CDS             13978..14652
                     /locus_tag="VGP50_07785"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEV8027317.1"
                     /translation="MIPDDLDDLHALAGEYVLGTLDPPAAREIDDALVANAALRRAVI
                     FWEERLHGLSRLAAPGEPPPEIWQKIAARLETAAQPRTRLWNSLTLWRWSTAAATAAA
                     ACLALYIALAPPAGPRLVAILHAPQQQQPAWLATAGSGGLLVRALATETAPVDRSFEL
                     WAIAPGATPVSLGLIPASGRLELGRSTSIRDGDLLAITIEPEGGSPSGQPTGPVVFAG
                     TLVAAK"
     gene            complement(14663..>15386)
                     /locus_tag="VGP50_07790"
     CDS             complement(14663..>15386)
                     /locus_tag="VGP50_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEV8027318.1"
                     /translation="DVLRAWLPFIVLVIVVVLWTGPWSGVPKYIPFKISVAAGSSIEA
                     GKTVSAAFNWAPGIGGTAILVSWIIVAFLVGLTGAQLREVFRRTWHQMWGACLVGVFI
                     FGLAFVFNYSGMANSLASGFSKIGVWFIVVAPVLGWIGVALSGSNTSTNAMFGAFQAS
                     VGRLLGFPLLLLPSLNSVGAEVGKPVAPQTASVGVSTSRFVRNEGAVIRHNMGWTFVL
                     LIYLVVIGVAFYYFAPGVLILH"
BASE COUNT         2567 a         5203 c         5143 g         2473 t
ORIGIN      
        1 gaaggtcacc gcattgccca tgctctggag ccacgtcgcg tattccttgc agggcgccat
       61 cggcgtgaag tcgtcccctt cgccgtgcag gaacagcatc ggcgccttgt cggtcgcgtg
      121 atcggcgaaa gcgacattgc agccggaata gagcgcgaca tgcgcggcga agtggagatt
      181 gccgccgcca aacccctggc gaagcgtctc gaacgcggtg tcgacggtga cgatcgcgcc
      241 gcgcgagaag cccatgatgg cgatgtggtc gcggtcgatg cgcgggtcgg cggcgagttg
      301 cttcagcgcg gcataggcgt cggcgacctg cgcaaaaatg gaaatctgct gttgatcgct
      361 caccgtctcg gtgatgccgc gcggcccgta ggtgtcgatc accagggccg ccacgcccca
      421 ggcattcaac cggccagccc attcgcgaat gtgggtaccc tcgtaatcga ggccgccgct
      481 gccatggacc aggaccacgg ccggcatcgg cgtgggtgcg ttcttgggca gataaagctc
      541 tcccggcaga ttgagcggct gcgacgccac cgtcagcttg ccggcgacga acaacggctt
      601 atccggccag tcccggccga ccgccggcgt catcgagggg atcgagagat cttcgaccgt
      661 ggctgtctcg gggccgacgg aagagcaggc cgcaaccagc agcgccagca gcggaatgat
      721 cgcgtgccgc atcaaagtct cctttgatcc aaacgcgccg ctcggatggg cgcagctttc
      781 tgtttatcac aatttgttaa gaaattggtt tcccctaacg gcggcatcgc ttcctccccg
      841 gcagccggat taacctcccg gaggccggcc gctgcgcgcc cctggcgcgg ccggcactga
      901 tccgcgatag tggatcaaac gagccgcccg cgaggtcgtc accatggcca agaagcccga
      961 ttgggaaata ccgatcctgg ggcagcccaa gcccgaggat ttcgccttcg acctcgatcg
     1021 ggcgcttctc tcggtggtgg gtcttcaggc gaatataccg gaggacgcgt ccaccgcctc
     1081 gatcctcggc acggagcgca gcggcagcgg cgtggtgatc cgcgaaagcg gtctcgtgct
     1141 caccatcggc tatctcatca tcgaggccga gagcctctgg ctcagcacgg ccgacggccg
     1201 cgccatcccc gggcacgtgc tcgcccatga ctgggagacc gggttcggcc tggtgcaggc
     1261 gctgggtcgg ctcggcgtgc cggcgctccc actcggccgc tcgagcgagc ttgcggcgaa
     1321 cgatcccgtg atccttgccg gtggcggccg ccggcaatcc gtccgcgctt cggtgatcgg
     1381 caagcaggag tacgcgggat attgggaata tctgctcgag gaggcgatct tcaccgcgcc
     1441 cgctcatccc ttctggggtg gcaccgggct catcggccgc gacggcacgc ttctcggcat
     1501 cggctcactc aacgtgcagc aggtgaccga ccgcggcgcg ccgcgcaaga tcaacatggt
     1561 ggtgccgatc gatgtgctga agccggtgct cgacgagctc ttgacctatg ggcggcgcaa
     1621 tcgcgcggca cggccgtggc tcggcctcta ctccgccgag agcgacggca aagtggtgat
     1681 cgccgcactg accgagcgcg gacctgcggc cgcagcaggc atgcgcgtcg gcgacgtcgt
     1741 ctgggccgtg cgcgacgcca gcgtcgaaag tctcgccgac ttctatcgca aggtctgggc
     1801 aagcggcgcc gccggcgccg agattccgat cgaagttctg cgtgacgacc gcaccgtctg
     1861 ggttcgcgtg aagtcggtcg accgtcaggc gctcctcaaa tcgccgcggc tgcaatagct
     1921 cgtctcttcg cgtgcgcctc gatcagaagg cgacttgcca gcgagcggca acggcgtcga
     1981 atttgaggcc cgtgccgatg ccgagcggcc ccgtcgtgac gcttccgctc tgtttgtcga
     2041 tgatgccgtg caagtagttg agcgagagac ggacataggt atcgacgtac caattaaggc
     2101 ccaaagtata gaccgtctgt ttgccgccag cgaccccttg tgtcgtggcc gcggcaacgc
     2161 cgggcgtgaa gcggtcgttg agatcgacga cgctgtagcg cgcggcaact tcgaaagcgc
     2221 cccacccgtc gccggtgaac gacatcggat ggtccggcag gataccggcg taagcgccgg
     2281 ttgcggggat gtagacgtgc ggctccccgg tgagcaccca gctcgcctcg gcgtagccgc
     2341 cgtcgaaatt gagtttcgcc aaggcgttgc gctcgatgat gtagtggtaa tactcgctct
     2401 gaaagaacag gttctcgtag gctgccgcca gctcggcgcc atacacttct gcgtttgtca
     2461 cttcgagcag cgatccggtg ttgagcacga cggtcggatc gatgcgcaat tccggccggt
     2521 cgctcaaggt caaggtcggt atgccattga cgttctgtgc cttgagcaag ctctcgacgt
     2581 cgacgccgag atgcagcgac cccgcggatg attgcagagc ctgaacggaa aatcggccca
     2641 aggcgccgaa ctgctcgccc acattgtgcg gctggcccga taccggcccg gtcgcccaga
     2701 ggccggcgaa atagcggtcg ttgatgtagc gaacgtcgac cgccgaacgg aagtcgccgc
     2761 cggcgatatt gaatgcgacg gcggccgagg acgcccgctc gaggaacagg atatcggtcg
     2821 acgacgtcgc ctcgtcgagc gtccagggca cgttttgata gccggcatcg atggcgagcg
     2881 gccgaaagcc tgtgtaggtg atataggcgt tctcgatccc ggacgtgttc gcacccgagt
     2941 tgacgaggct gtcggccgag ccgccgaaat catagatcaa cgcatagtcc caatcgccat
     3001 agaacttgcc gacgattccg agacgggccc ggcgcgcatt gacgccgctc gtgagatcgg
     3061 atggcgagct gaatttcgac tgtggcttta cgttgaaata gtcgccgaga tcgaactgca
     3121 cgcggcttgc cagctggatc gaggtttgcc cgtcggcggt ggtgaaggcc ggaccttttg
     3181 ccgggtagag cgtgagccgc ggctctccgg gaacggtcgt gctcggaggc gcggctgcct
     3241 gcgtcggcgc ggccgccgcc gggggcgcgc ttgccgccgg ggcctgggca ggaaaggctt
     3301 ggtttttctg catctgactc tggagatcct gcagctgctc ctgcagccgg tcgatctgtt
     3361 gctgcgattg ctcccgcagc tgctggatct gctgctgcag ctgctcgatc tgatccgccg
     3421 atgtttgtgc ggcggctggc ctgatcagaa gaagcgcggc agcgccaagc gcggctccgc
     3481 cggcaagatg acccctcagc gacatcgatt ccccccttca atcggcacgc agcagcgtgc
     3541 gcggcataaa tacatatata acacgtctat tggcaataaa atactagaca gaatgctggt
     3601 tcattctatt tctacaatag tcattagttg tatatcggcg gccacgcacc caccctgcgc
     3661 aagaagctcg gcgtcgcatt tccgcaatcg caatccgcac gctcgctcgc gaggcgttgc
     3721 agctcgagga caagatcggc accatgtgcc cgacaacgtc gtcgtgcagg aactcgacga
     3781 ggacagaatc ggggtcgtgt cgatcggccc tagggaacgc ggggaaagtc acgatgagcg
     3841 agcacgtctc cgtcaccatc acgcgccagg acaagtatcg gttcctggtg gatttcgggc
     3901 caggcatcga gaaggcggtg acggacgagc cagcgccgct cggcgacggg gccggtcctt
     3961 cgccgcagca actgctcgcc gccgcgatgg cgaattgcct ttccgccagc ctcgttttcg
     4021 cgatcgccaa gttcaagggc gatccgggac cgctgacgac gacagctttg tgcgagctcg
     4081 gacggaacga gaggaaccgg ctgcgcgtca ccgcgatcag cgtcaccatg acgctcggca
     4141 cggcccccga atccctcgaa cacctcgacc gagccttggc gcaattcgag gatttctgca
     4201 ccgtctctca gagcgttcgc gccggcatcc ccttcagcgt gaccgtcaaa ggcccggacg
     4261 gccgtctcct gaagtagccg tcacgtcacg gcgtgggtcg cggcggtacc tgcagccaag
     4321 gctcgggaca ggacgagacc tgggggtagt aggcctgtgc cgactggcag aaataccagt
     4381 accccgccgg cggcgcgccc gtgtcatcag gcgctgccgc agccggcggc ggcgcagcag
     4441 gcggcgcctc ggccatcgga gcgctgtcgt cgtcctcgcc cggtgcggga tacgcgtagg
     4501 caggcgcggg atacgcgtag gccggatagc cgtaataggg gtagggatag ccataggcat
     4561 aggccggata gccccaccac aacggatcgt agaagccgcc gaacgcgaag ccgccgaaga
     4621 accggcgatg gaagccgttg tgaaatcggc cgtcatgaaa accgccgcgg aagccgcggc
     4681 cgccccagcc gccatggcca ccccaactgc cgcgaccacc gccaccgccc ccatggccgc
     4741 cgccgccacc gccgccgaaa gccgcggccg atggggcgtc ggcgagcgtc accgccagcg
     4801 cgaaggctgc tacgaccatc gaggccgttc cgatcttggc tttcatggcc tcctcctgtg
     4861 tcgtgtagag cacaaaacaa acagatgggc ctcgttcaat gatgccacaa gtatgcccgg
     4921 aggacgtctc aaatcgtctc gacggcaact tgcgacaacg gggcaaggcg ccggcgcgtg
     4981 gcgcggcacg tcccgcggcg gccctcgccg atgatcgtct aagctgttga ttgccttgcg
     5041 ccaaagcgac cccgccatga gatccccggg ccgaagagtc gcgacacttc aacgcgtacc
     5101 cgccctaccg acgcaaatat tgcgtgccca gccacgatgc ggcggttaac aaattttaca
     5161 catcgcagaa ttgtgatcgt ccgctcattg gacgatggtg accgcgcggc ggcgatccct
     5221 gccgccacga ggttctgctt cgcggcatag gcgcggctac cgcgccgcga ccgctgagcg
     5281 cgaccccgtg aggctggcga tgagtaccgc cagcacgccc gccaagaaga tgccatcgaa
     5341 cgtgccggcg ccgccgatcg aggcgaccgg cgcccccagt ccctggatct tgtcgagatt
     5401 gagcaggtcg gcgccgatga gcgtgccgag gctgccgccg atataagcga gcggcgcggc
     5461 acggcgcggc gacagcagca gcgcgacgac cgctgcggcc agcgccggca cgaagacggg
     5521 cagtgcgatg cctagtcctg gaacgggatc cgccaggaca tggcacatga cagcgacgca
     5581 ggcggtcgcc atcgcgccgc ggacccacag ccgatgcctg gcaagcagat agagcgacat
     5641 ggcgccggga atgagggcgc cgccgacatt gaccgcgatc accgtgcccg gcgaatcgac
     5701 caggaccggc acgacatagc gcatcccgta gaaagtgatc tcccgtcccg ccgcgagctg
     5761 gtgcccgcgc agctcggcca ccgggatatt gaagtagctt ccgatgagcg acccgagaag
     5821 caggagcatc gctgcgccgg ggctgatgcc gaggcgcaga taggcgtagc gcagcactcc
     5881 gatctgcagc agcgcgacca gcacgaggaa cacggcgacc agcatcgaga agaacggcag
     5941 ggcgagcggc aggtaatgga gatgactgaa agacatcgct ggcgctccgc ggcatcgcgc
     6001 tctgctggag gggaacctat atattgatcg acatggcgct ggccggtaat atggcgggag
     6061 ttgcgcggct cggccatacg cggacatgat ggaggccggc aatgaagtgc gcagtaaggt
     6121 gtctctcgtc catgctcgtc ctcggcgcgt cgtcctgcgt cgcgccgccc tacgccgaag
     6181 tgaccgactg cgcggatttc gtccagacgc ccagcgagta ccacatggaa atcgccgggc
     6241 gcgccggcat gctgacccag caggaacaca gtttcgtctg ctacgccggc gttctcggcc
     6301 gggcgcaagt gacgtcggcg atgatggcgc agcaacgcgg cgtgagcccg gccgtgacgc
     6361 gcttcgcggc gatcactgcc ggagaccagg agatgctcaa ccggcgcctc gccgccaccg
     6421 ccgagcagca tgccgggctc attccgccgg acggcctcga cgcgcctcat cttgccatgc
     6481 tcgaccagct gagccagctc tcgggtgagg ccttcgaccg ggcctatatc gaggatcagc
     6541 ttcaggaggc gcaggcgacg atcgccgtcc tgcaggaaga aatggccgaa ggcagcgagc
     6601 cgacgctgcg cagcttcgcg cgcgacgtcc ttccttatgt cgaagagcgc gcacgcgaag
     6661 cgcagagcat catcgagcaa ttgcccggtt gacgcttgcc ggccatcgcg ccgccgctat
     6721 gtcaccatcg cggcgctatt gaccggcgtc accggcggga ggcagggccc cggactgcag
     6781 ggaccgcccc tggcccgcgg atgaagtcgt cgcgggcgcc atggcgcagg cgccgagcaa
     6841 ggacacgctc cccaacagaa ccgcgacgaa cagcaacgat gtcgatgatt tgcgatacat
     6901 ggctcgcctc cgaaatatga acgaggccgc cttcccccgg cgcatgaacg ttgccgcgct
     6961 ctggcgcccg agctgccgat gaatcgaaga tggaatggcg cggatcgctc ttggaggcct
     7021 ctcgaccaca tgaccgtttt tttatggtcg tcggattgtc gagaattttc gcctgttcct
     7081 gctgagcgcg ctttcatggc gcgagtcatg aggcggcgga ataggcgaag cgcgcccgct
     7141 cgcgtttaca cggacgacat cggtccatat cgtgcggagc gacggacagc gctctgttcc
     7201 gctcgaggaa aggcagctcg atgaaaggcc tggcgcggct tcttacgcca cacctccact
     7261 atgcgtgggt cgtcgccggc gtggcgttcc tcaccttgct ggccgccgtg gctgtccggg
     7321 cgacgcccag cgtgctcatc gtgccgctgg agcaggcctt cggctggagc tccggcacga
     7381 tctccttcgc catcgccatc aacatcgtgc tctacgggct gatggggccg ttctcgggcg
     7441 cgttgatgca gcgtttcggc atacgccgca ccgtccttgt cgccttgacg ctcctcgccg
     7501 ccagcgtcgg cgccagcagt ttcatgaccc agccctggca gctcgtgctg agctggggca
     7561 tcgccgtcgg tctcggcagc ggcgccacgg cgctggtgct gggcgcgacc atcgtcaatc
     7621 gctggttcgc cgagcggcag ggcgtggtga tgggattcat cactgcgagc acggccaccg
     7681 gccagctcgt cttcctgccg atgctggcgt ccgtggccga caaggccggc tggcgcccgg
     7741 tcgtgtggat cgcggcggcg gtcgccgccg ccatgatccc gatcgtagcg ctgctcctgc
     7801 cggacagccc cgcagacatc ggcctcaagc gctacggcgg ggaggaggaa agcgacgccg
     7861 ccgtcacgac ccggacgaat cccctgggca tcgcgctctc ggcgctcgga cgggccagcc
     7921 ggtccggcgg tttctggctg ctcttcgcca gcttcttcat ctgcggcgcc agcaccaacg
     7981 gactgatcgg cacgcatctc atttcttatt gcatcgacca cggcatgccc gcggtcgaag
     8041 gcgcgacgct gctggcggcg atggggatct gcgacatcat cggcaccacc ggctcgggct
     8101 ggctctccga ccgctacgac agccgatggc tgctcttctg gtattacggg ctgcgcggcc
     8161 tgtcgctgct ctacctcccc tattccggct tctcgctttc gggattgtcg atcttcgcgg
     8221 tgttctacgg cctcgattgg ctcgcgaccg tgccgccgac ggtgcgcctc acccgcgatc
     8281 tcttcggcaa gacggaagcg cccatcgtct tcggctggct gctaacggga catcagctcg
     8341 gtgccggtct caccgccttc gaggcgggga cgctgcgcgc ggcactcggc agctatctcg
     8401 tgccggtcct catttcaggg gcgctctgca tcatcgccgc ccttctggtg ctgcgtgtcg
     8461 gacgcaaccg ttcgacactc gagccggcgg cgggaaccgc gttctgaggc tcgcgccgcc
     8521 gcaatcagcc ggtcatcatc cgcacgaatt cgcccagtct ggcaatcgcg tcccggccct
     8581 cgcgaagctg ggctgcgaac cagtgccaga catggatcat ctccggccat atctcgagcg
     8641 tcgtgcgcac gccctgctcg cgcgcgcgac ggtcgagctc gcgcgcatcg tcgagcagca
     8701 cctcttcgct tccgacctga atcagcaagg gcggcaggcc gcgcagatcc ccatagagcg
     8761 gcgaggcgag cgggttgcgc agatcgcccc cgaccagata atgacgggcg cactcggcga
     8821 gagagtcttt cttgacgatc ggatctctgg cggcgagcgt ctcgaggctg acgaggctgc
     8881 agctgagatc gacccaaggc gagatcagcg ccgcgccggc cggaagcggc aggccgcggt
     8941 cgcgcgccgc caggagcgtc gcaaccgcga gcccgccgcc ggcggaatcg ccggcgatca
     9001 ccaggctcgt tggcgcctgc ccgccatcga gcagcccgcg ataggccgcc agcgcatcgt
     9061 cgagcgccgc cgggaacgga tgctcggggg cgaggcgata ggcgggcaac agcacactcg
     9121 cctgcgccgc ttcggcgatc gccgccgcca gatggcgatg ggaatcggcc gaccccatga
     9181 catagccgcc gccatggaga tacagcacca cgcgccctgc tcggctggcg ggcatcgcga
     9241 tgcactccgc cgcgacgccg cctgcgtcca cccgcgcgac ggcgacgccg gcaggcagcg
     9301 ggaagaggtg cttcgcgcga tcatagcgcg tgcgtcgctc ggccagcggc aggctgtcga
     9361 gcggcggcaa tgcgccgaga aaagcccgaa cttgcttgat gtcgtcgctc acggcgcggc
     9421 tccggcagat tgagaaatgc ccgacaacca taagcggcgg gggtgacggc gagcaacatc
     9481 gatcgcgtgc cccgcggcgc gatccgggct aaattcccgg ccatgaagaa cgaacgctgc
     9541 gcggcgccgc gatcgacgct acgtctcgcc gcgacgaaca ccggagaatc tccatcgtga
     9601 cgccgcgcgc cattgccatc gactggcccg cggacattca cgcggcgctg agcgcggccg
     9661 acattcggca agtcgcctct gtgcccgatg ccggccatgc gcggctcatg acgctctgct
     9721 cggccgatcc gcgcatgcgc atggtgccgc tcaccagcga gcaggaaggg atcggcctcg
     9781 ccgccggtgc ctggctcggc ggccaacgtt ccgtgctgct catgcagtcg agcggcgtcg
     9841 gcaactgcat caatgcgctg gcgctgatcg agagctgccg cttccccttc ctcaccctga
     9901 tcacgatgcg cggcgagttc ggcgagttca acccgtggca agtgccgatg gggcatgccg
     9961 cgggcccgag cctcgagctc gccaatatca tcgtccatcg cgtgagcggc gccggcgagg
    10021 ccggcgctac cgtcgcgggc gcggcgcggc tcgctttcgc gagctatcgc ggcgtcgccg
    10081 tgctgctgtc gcagcagctc atcggcgcca agtcgttcgg ccagcagcga tgacaacggt
    10141 ggctctcgat cgccgcgccg tcgctggggc gctgcttgcg caacgtggcg atctgctcgt
    10201 cgtcgcgggg ctgggggcgc ccgcgtggga cgcgacggcg gccggcgatc atccgctgaa
    10261 tttcccgctc tggggcgcga tgggcagcgc cgccatggtc ggacttggtg tcgccttggc
    10321 gcagccgtcg cgtccggtgc tggtgctgac gggcgacggc gagatgctga tgggtctcgg
    10381 cagcctcgcc accatcgccg tgcagcggcc tccgaacctc gccatcgtcg tcctcgacaa
    10441 tgagcgctat ggcgagacgg gcatgcagcc gacccacacc gcccatggcg tcgatcttgc
    10501 cgcggtcgcc gccgcctgtg gcttcgcggc gagcacgacc atccggacga tgaccgaggt
    10561 ggcggcactg ggcgcaacgt tgcgcgacgg ccgtggaccg cgcttcgcac aagtgaagat
    10621 cgcggcggac aatccgccat tggtgttgcc gccgcgcgac ggcggccttc tcaaggaccg
    10681 ctttcgcgcg gcgctgctcg gcccggacgc ggcgcttatt taactattgt gggatcaccg
    10741 ggtggcgcgc cggacgcccc gccagcactt ccaggacctg gtccacgacc aggaagccga
    10801 cgcgctcgag cgcctcttcg gtggcggcac cgagatgcgg cgtggcgatg aagttcggca
    10861 gctccagcaa gggatgatcg ggaggcggcg gctcgagcgc gaagacgtcg ctcgccgcta
    10921 cggccggccg gccgcgccgc agcgcgtcgg cgagcgccgc ttcatcgacg accccgcccc
    10981 gcgcggtgtt gacgaggatg ctgccttcgg tcatgagcgc cagctcgcgc gcgccgatga
    11041 ggccgagcgt tgccctcgtc agcggcacat gcaggctcag caggtcgctc tctctgagca
    11101 acgcctcgaa cgaggccgcc ggcgcagcac cgccggcgcg gatcgtctcg cccggtaccg
    11161 ccgcgtcgta agccgccacg gacatgccca gcgcgcgggc gaagagcgcc gcgacgcggc
    11221 ggccgacctg cccgaagccg acgatgccga gccgccgtcc cgcaagttcg cggccattgg
    11281 gcaatgctgc cgccgcgaga ttgcccaggc gcagcgcgcg atcgcccgtg acgatgtggc
    11341 gcgccgcggc gagggcgagg ccaagcacga gctcggccac ggcatcgcca ttggcacccg
    11401 gcgtattgac gacggggatg ccgcgcgccc gcgctgcggc gaccgcgatg ttgtcgacgc
    11461 ccactccgtg cttgccgatg atcttcaagt gccgcgcgcg ctcgatgtcg gcagcaccga
    11521 ccgggctggt gcgagtgatg acggcgtcgg ccttttccgg ccagagcgca atggcagggt
    11581 cgcgccagtc gacgacgccg ctcccgagtc gggcgagtgc ccgcggactg atcgggtcga
    11641 gcacgaatac cgccacgaat cctcgtcctc cacgtcggcg cccacggcgc cacgtcacag
    11701 aagcatagcc ggagtctgat tcggcgcgaa agcgccaagc cgacggcgcc aagccgattc
    11761 ggaagtgctc tcgccttccg cgagatgagc gcgtaaggta agtgggctgc gctggggcgg
    11821 cgcggctgct gcgtgagaga tgtacgcagt ctttcggggg tcgctggggg atccattgca
    11881 tctgctctcg gtcgcgcgga tggcgacgta gcgtgacggc gattccgcta ccgccgtcgg
    11941 ccgtcgattg gcggcgcgtc gagcactgga ccgccgagat tgcgggcggt tctctgcgcg
    12001 cgctgcgaaa aacctttggc cagggtcggg gcgggccgag catcctctgg cgcccgccgc
    12061 tgtcgcggct cgcggcgccg cagttgcatt tcctgatggg ctattggaat ggactcgccg
    12121 gggcacgaac gatgccagag gcgcgcgaga tcgacgcctt ggagatgcgg cccgcgctcg
    12181 gctatgtgag cttgctcgag gtcatcgagg acggtcatga tttccgttat cgagtcttcg
    12241 gcagcgccat tgccgccgtc gtcggttttg acatgacggg acggccggcg tccgccctca
    12301 aggcgagagc cgaggtggca gaattcgccc tcgccatcta ccgcgccgtg ctggcccggg
    12361 gcgaaccgct tctcgcggag cacgggccgc cgtccaccat cagcagcgtg gcctggcaca
    12421 gcctcgtctt gccgctggcc gaccccggcg gcgccgtcgt ccgtctgctc gccggcgcgg
    12481 tgcctgtggc acgcgacggc cgggcgctgc tgccgcgact ctgacaacga tcaactttct
    12541 gtgattttgc gcgccgccgc atccacgcgg tcgcgtcgcg agtatttccc gaagcggcgt
    12601 ccattccggt agcggtaaag ggcgctttcg atgccgccgc gccgaccgcc gcccagagga
    12661 ccaacaatga ccgcgttctc cacactctcc cgcctgactc ggatcggcgc cttcggcatc
    12721 gcttgcagcg tcatcgtcgc ttgcgcgcaa ggcaatgccg agacgaattc cgagcccatg
    12781 gcgatgcacc acgccatgac cgccgcgagc tccaaggcgg ccgatctgcg cagcgcgctc
    12841 gacgaattgt tgagcgagca cgtcatcctt gccgcagagg ccaccaacgc cgccctggcc
    12901 gggcggcagc ccgatttcca ggccgcggcg gcggcgctcg accagaactc gatcgccatc
    12961 tccaaggcgg tgggctccgt ctacggcgcc gaggccgagg cgcagttcct gccgctctgg
    13021 cgcagccata tcaacctcgt cgtcgactac accgtcggca cggcgtccaa cgacccggca
    13081 aaagcgcagc aggccgtcaa caagcttctc gcctatagcg gcgatcttgc cggcttcttc
    13141 agcggcgcca atcccaatct gtccaaggag gcggtcgccg acatggtgaa ggagcacatc
    13201 gtcaccctca aggcggtgat cgatgctcag gcgaagaagg actacgccgc cgaatacacg
    13261 gcgcagcgca ccgcctatca tcacatgggc atgatggcgc ttgccctcgc cgacggcatc
    13321 gccaagcagt tcccgcagaa attctcctga agcagcgcgc ggcgggcggc ccttggccgc
    13381 ccgtccgcca tggacaccgc cttgctttcc ggccacgctg atggtgatgg ataacgagat
    13441 cgtcgccttg tcctcgctcg aggccctggt ggaggcggtt gcgcggggca gccagcctgc
    13501 cttgcggcgg atctatgagc aggagtcgcg tcgcctctat ggcgttgcgc tccgcatcgc
    13561 ccgccgcccc gcgctggcgg ccgacgcgct ccaggacgct ttcgtgcagg tctggcaaaa
    13621 ggcgggaagc ttcgccgccg agcgcggcag cgccgaggcc tggcttacgg ggatcgtccg
    13681 gtatcgcgcg ctcgacgccg tgcgcaagac gagccgcgag gagccgtccg acgatccggc
    13741 gctgggcgac cgcgccgagg acccggacca gatcgagcag atcgaccgcc tgctgagccg
    13801 cgacgcgtta cggcgctgcc tcggcgcgct cgacgagaat cagcggcgct cgatcctgct
    13861 ggccttcgtc gacgggctct cccatgctga gatttccaaa cggttgccgg cgcccctcgg
    13921 cagcgtgaag agttgggtgc ggcgagggct tctctcgctg cggagctgtc tcgattcatg
    13981 atccccgacg atctggacga cctgcatgcg ctcgccggcg agtatgttct gggaacactc
    14041 gatccgccgg cggcgcgcga gatcgacgac gcgctcgttg ccaacgcggc actgcgccgc
    14101 gcggtgatct tctgggaaga gcggttgcac ggcttgtcgc gcctggcagc gcccggcgag
    14161 ccgccacccg agatctggca gaagatcgcc gcacggctcg agactgccgc gcaaccccgg
    14221 acgcgcctct ggaactcgct cacgctgtgg cggtggtcga cggccgcggc aacggccgct
    14281 gccgcctgcc tcgccctcta tatcgctctc gcgccgccgg cgggcccgcg tctcgtcgcc
    14341 attcttcacg ccccgcagca gcagcaaccg gcttggctcg cgacggcggg aagtggcgga
    14401 ttgctggtgc gcgcgctggc gacggaaacg gcgcccgtcg accgctcttt cgagctctgg
    14461 gcgatcgcgc ccggggcaac gcctgtgtcg ctgggcctca tccctgccag cgggcggctc
    14521 gagctcggcc ggtccacttc catccgcgac ggcgatctgc tcgcgattac gatcgagccc
    14581 gagggcggct caccctcggg acagccgacg ggacctgtgg tgttcgccgg gacgctcgtc
    14641 gccgcgaaat agcgccggtg cgtcagtgca ggatcaggac ccccggcgcg aagtagtaga
    14701 acgcgacgcc gatgaccacg agatagatca ggagcacgaa ggtccagccc atgttgtggc
    14761 ggatcaccgc gccctcgttg cgcacgaagc ggctggttga gacgccgacg ctggcggtct
    14821 gcggcgccac cggcttgccc acctcggcac cgaccgagtt gagcgagggc aggaggagca
    14881 atgggaagcc gagcagcctg ccgaccgacg cctgaaacgc gccgaacatc gcattggtgg
    14941 acgtattgct gcccgacagc gccacgccga tccagccgag gaccggcgcc acgacgatga
    15001 accagacacc gatcttggag aagccgctgg ctagcgaatt ggccatcccg gaatagttga
    15061 agacgaaggc aaggccgaag atgaagacac cgaccaggca cgcgccccac atctggtgcc
    15121 aggtccttct gaagacttcc ctgagctggg cgccggtgag gccgaccagg aacgcgacga
    15181 tgatccacga caccaggatc gccgtgccgc caatgccggg cgcccagttg aaggcggcgc
    15241 ttaccgtctt accggcctcg atcgacgatc cggcagcgac ggagatcttg aacggaatgt
    15301 atttcggcac gcccgaccac ggtcccgtcc acagcacgac gacgatcacg agaacgatga
    15361 acggaagcca ggcccgcagc acatcg
//
LOCUS       DASYNS010000248        12171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_837374, whole genome shotgun sequence.
ACCESSION   DASYNS010000248 DASYNS010000000
VERSION     DASYNS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837374"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="VGP50_07795"
     CDS             complement(<1..1488)
                     /locus_tag="VGP50_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEV8027319.1"
                     /translation="MSRVQDPYWAAQGLGGPLRLEDHYGARRMLCFSERPETLASMFA
                     DLVRRFPRRPALIEQRTLTYAELDGIVASIAAGLAREGIGHGDRVALLLGNRWEFLAI
                     LLACARLGAIVVPIGTRQRRAEVEFLLDDCGAKALIFEAEFAAAVPQPGALRQKLGLF
                     VLHGAALGAAPFADLLAEGSATAPLHPTAEEDTAVILYTSGTTGRPKGAQLTHLGIIH
                     SALTLARCHGLTAEDRGLVAVPLSHVTGLVGVSLATMIVGGCVVLMQQAYKTPDFLEL
                     ASRQRITYSLLVPAIYTLCALSPELGKHDLSVWRIGCFGGAPMPVSTIELLAQKLPRL
                     ELLNAYGATETTSPATLMPRRCWREHMDSVGQVVPCGAIKVLDESGQPVPAGEPGELW
                     ISGPMVVPGYWGRPDANRSEFSDGFWRSGDIGSIDRDGFVRVFDRRKDMINRGGFKIF
                     SAEVENVLSGLAGVRECAVIGYPDPVLGERVHAFVVTDDRSGLTPE"
     gene            complement(1485..2153)
                     /locus_tag="VGP50_07800"
     CDS             complement(1485..2153)
                     /locus_tag="VGP50_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8027320.1"
                     /translation="MTSGLAVVDLRVSIQSVEVLRGLSLAVAPGEMIGLVGRNGAGKT
                     TTLRAVMGLLPADGEIRFAGVDLARQPTHGRAGLGIGYMPEDRRLVPLLSVEENILLP
                     SWATRDPEGKARLAFVYGLMPEVAAMRARKALLLSGGQQKLVALARALMAGRRLLLLD
                     EPFEGVAPALSQRLAEVIAALKGTELGVLIAQSDLNHSARLVDREYLIERGANRARVA
                     EATA"
     gene            complement(2150..2899)
                     /locus_tag="VGP50_07805"
     CDS             complement(2150..2899)
                     /locus_tag="VGP50_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027321.1"
                     /translation="MTALIEAKGLHKAFGAVIAAKDVSVAARRGERVGLIGNNGAGKT
                     TFVNMVTGYIKPDRGSILLDGVDITGLAPRRVSRHGVSRSFQMPQLFASLTPEENLLM
                     ALGIGRPEGLSAWRPARTRALVAELEELLARFRLAEYRGHAIAALPGGVRKLVDIAMA
                     VTRRPKLLLLDEPTSGVAAEEKFPLMNVIMAALAGADVTVLFVEHDMEIVERYAERVL
                     AFHDGRVIADDAPAAVLGNADVQRHVTGIVQ"
     gene            complement(2896..3867)
                     /locus_tag="VGP50_07810"
     CDS             complement(2896..3867)
                     /locus_tag="VGP50_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8027322.1"
                     /translation="MRRFAPFAIALTSFALLALLGRAAPSWVVFLATMAFAKGLVALG
                     LVLLMRGGLVSFGQGLFYCAGAYAAGLAMRWTGDALILVAIGGIAALLLGLAIAPLLA
                     GYRSIFFATLTLALSMMAYGVLNKTYALGGSDGINLHAPSFLSYRPGTSQALGYALFL
                     LSAASTVAAAVLCRVHFDSMRGLLSLAARDNEIRVEYLGASVRRVAFGNFVAAAALGG
                     VGGALAALALGHVDPDFAFWTSSGEFVFLAILAGHQSVVAVFLASILLELVRSFSSQY
                     FPNSWQMALGLFLLLVILFLPEGLGSLLRRRRGAAAESVTRPLESLR"
     gene            complement(3867..4763)
                     /locus_tag="VGP50_07815"
     CDS             complement(3867..4763)
                     /locus_tag="VGP50_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8027323.1"
                     /translation="MELAATIVIDGVIDASWLFIVAVGLTLVFGVLNILNIAHGNLYA
                     IGAYTAASAVGWYYSGSGPPLLGYIVMLGAALLVAVVVGPLLERGILRLFYRRDEIVL
                     VLVTYALFLILEDATTLIWGVNPYYAYEPYGLLGNVNLLGLTYVGYDLLLVALAGITG
                     VLLWLGLNRTITGKIVLAVIHDREMSRAMGVNVTRVFVLAFTVGVFLAALGGAFTAPK
                     ISVQPGISITVILLSFAVVVIGGLGSVEGAAIAALLVGLARSAAIHLMPSAELFIVYL
                     VMAGVLALRPEGIFQRIALRKI"
     gene            complement(4801..6111)
                     /locus_tag="VGP50_07820"
     CDS             complement(4801..6111)
                     /locus_tag="VGP50_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027324.1"
                     /translation="MSYRKSMLGFAAIAAAAAAMTAGEAAADENSFKLGVVTFLSGQA
                     AESFGVPAWNSGKMVIDALNKGGVLPKPYDKPGFGGLKIEAVVIDENGGATKQVQELR
                     NAYERDQVDAIVGYVGSGDCLAVSPAAEELKKLLILYDCGTPRIFEENKFHYVFRTAA
                     HGAMDNVAMARYIAKRKLNIGDMGAINQDYAWGQDSWKDFKASMAQLDPDAKATAELW
                     PKFGAGQYGTEISTLLQEKPSLVYSSLWGGDLQALVLQAVPRGLFKDRYVVLSASDHV
                     LQPLGDKMPDGVIIGARGANGQFAERSPLNDWFVDAYKKAYPGTYPVQPQYRMIQALL
                     GIKTAVETAMAANGGKKPSTEELAAAMTGLEWDAPSGHIKMALGDGHQAIQPNAVGRT
                     QWDAQAKMVKLVDIEHFIAECVNPPANMKAEEWISKGFPGAQCE"
     gene            6507..8243
                     /gene="poxB"
                     /locus_tag="VGP50_07825"
     CDS             6507..8243
                     /gene="poxB"
                     /locus_tag="VGP50_07825"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011633049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HEV8027325.1"
                     /translation="MARSVADQIAETLALAGVKRIYGIVGDSLNGLTDAIRRQAKIEW
                     LHVRHEEVAAFAAGAEAHLTGDLAVCAGSCGPGNLHLINGLFDCHRSRVPVLAIAAQI
                     PSPEIGSGYFQETHPQSLFQECSHYCELVSSVNQMPRVLEVAIREAVGKRGVSVIVIP
                     GDVALQAAGDAPAAKLGGLLPPRPVVTPDPRDLDRLAALLNGDGRVTLLCGAGCQGAH
                     DQLLALGERLKAPMVHAMRGKEHVEWENPYDVGMTGLIGFSSGYYAMLDCDVLLMLGT
                     DFPYRQFYPERPGVRIAQVDLRAENIGRRAPVDLGVVGDVRATIAALLPLLQQKRDAT
                     HLAQAREHYAKARKELDRLAMGTPGKRLIHPQQIAKAVSDQAAGDAIFACDVGLPTVW
                     SARYLAMNGKRRLLGSFLHGSMANAMAQAIGAQAAFPGRQVVSLSGDGGFTMLMGDFL
                     SLKQLSLPVKVVVFNNGALGFVELEQKSTGFLDFGTELQNPNFAAMAEAVGVRGIRIE
                     DAADVEAGIAAAFAHDGPVLVDAVVNRTELAMPPSITVEMAKGFTLYMVKAVFNGRAD
                     EIIDLAKTNLWR"
     gene            complement(8518..9765)
                     /locus_tag="VGP50_07830"
     CDS             complement(8518..9765)
                     /locus_tag="VGP50_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV8027326.1"
                     /translation="MTLPAVPSGHRDLQDLRKLSHSPILTYLRKQAPPPAPRIKGLRA
                     RRPSPFPSMAAFSPSRIWTWFCQYLGHRIGRRHPFPTYAKGDSDQGIYPLDGDGGEIR
                     VALAADWGTGTDEAAAVANRIADFKPHYAIHLGDVYYVGTPTEVNANFLGIRNPATHY
                     VPCRWPSGSRGTFALNANHEMYALGYGYFDGILPAMGLVRDGKAQGQRASFFCLENEH
                     WRVIALDTGYNSIGIPIIEYFRQPSSALPPETVEWLREVVRPRADDPRGLVILSHHPY
                     FSCFDYCYPTPARQAAAFVKGPVLWFWGHEHRLAIYEEFDKPGGLRTFGRCIGHGGMP
                     VDLPPAKPLHPEFGVEFTDKRLYPNDENLTVGFNGFARLTFEGALLTVQYVDLRGTVV
                     FSETWAVEHGNLNRVRYSHLEAS"
     gene            10055..11383
                     /locus_tag="VGP50_07835"
     CDS             10055..11383
                     /locus_tag="VGP50_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV8027327.1"
                     /translation="MARVSNSFRLIATLTLALTVAAGDAVAQAETAALSSSAEQLYGA
                     ARPKLLQIRTLLVATGRQSSIGSGFLAGEDALAITNYHVVSQAALEPDTYRLEYVGAD
                     GHKGELKLLAIDLPNDLALVRLDQSGAGFFELGDTAGEPPKGERLYSMGDPLDLGFTI
                     VEGTYSGLVERSYNERIHFTGALNPGMSGGPAVTGDGRVAGVNVAKQQGGELVSFLVP
                     VRFAAALLARARGAEPLEPAAFHAEIGRQLAAWQARLYASFAERGFRAAGFGPYQAAE
                     STAPWFTCWSQTNAGQVPKPRASVDVTNCNSDTQLFIAGDLTTGLIHIGHSFVRALDL
                     NAFQFAAFLTQQAQTPWLGGFGRKWLTQRRCHEDFVTATPEGEHPALRAVWCARAYRE
                     FAGLYDVSVTAVTEDRGSEALVSRLTLQAIDYDDALKITRLFLEDMQWTR"
     gene            11371..>12171
                     /locus_tag="VGP50_07840"
     CDS             11371..>12171
                     /locus_tag="VGP50_07840"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV8027328.1"
                     /translation="MDQVIWVEILSRHREVVARHRFAAAELHIGRGYDNDLVLDDPHV
                     AVNHIRIYRGEAGDLMAEDIGSANGLFIDRDQRRYERVALDGDRVIRIGGVQLRVRER
                     GYAVPRERVQHPERRLWPVALALALAILGIQALGLWLAETGEVRASRYVVPLLSIAGP
                     ALVWTAVWTVLSRVFAGRAAFERNLITALAGLLAYSLYDEFAQFAAFGLSWRLPIAYE
                     YVAMWAVVALVCYRHIETLGARRRLVAAAGAGVALLGIATQSLTQSEAR"
BASE COUNT         2064 a         4136 c         4065 g         1906 t
ORIGIN      
        1 ctccggtgtc agaccggagc gatcgtcggt cacgacgaag gcgtggacgc gctcaccgag
       61 caccgggtcg ggatagccga tgacggcgca ttcgcggacg cccgcaagcc cgctcagcac
      121 gttctcgacc tcggcgctga agatcttgaa gccgccgcgg ttgatcatgt ccttgcggcg
      181 gtcgaagacg cgcacgaagc cgtcccgatc tatcgaaccg atgtcgcccg accgccagaa
      241 tccgtcgctg aattcgctgc ggttggcgtc gggacggccc caatagccgg gcacgaccat
      301 cgggccgcta atccataatt ctcccggctc ccccgccggc acgggctggc cgctctcgtc
      361 caggactttg atcgcgccgc acggcaccac ctggccgacg ctgtccatgt gctcgcgcca
      421 gcagcgccgc ggcatgaggg tcgcgggcga cgtggtctcc gtcgcgccgt aggcgttcag
      481 cagctcgaga cgcggcagct tctgtgcgag cagctcgatc gtgctcaccg gcatcggcgc
      541 gccgccgaag cagccgatcc gccacaccga gagatcgtgt ttgccgagct cgggcgacag
      601 ggcgcagagc gtgtagatcg ccggcacgag gagcgaatag gtgatccgct gccggctcgc
      661 cagctccaga aaatccggcg tcttgtaggc ctgctgcatc agcacgacac agccgccgac
      721 gatcatcgtc gccagggaga cgccgaccag gcccgtgaca tgggaaagcg gcaccgcgac
      781 gaggccgcga tcctccgcgg tgagcccatg gcagcgcgcg agggtgagcg ccgaatgaat
      841 gatgccgaga tgcgtgagct gggcgccttt gggccgtccc gtcgtccccg aggtgtagag
      901 aatgacggcc gtgtcctcct ccgcggtcgg gtgcagcggc gccgttgccg atccttcggc
      961 cagcagatct gcgaaaggcg ccgcgcccag cgccgcgcca tgaagcacga agagaccgag
     1021 cttctggcga agcgccccgg gctgcggcac cgccgcggcg aactcggctt cgaagatcag
     1081 cgccttggcg ccgcaatcgt cgagcaggaa ctcgacctcg gcgcggcgct gccttgtgcc
     1141 gatcggcacg acgatcgcgc caagacgcgc acaagcgagc aagatggcga gaaattccca
     1201 gcgattgccg agcagcagcg cgacgcggtc gccgtgccct atgccctcgc gagcaagtcc
     1261 cgcggcgatc gacgcgacga tcccgtcgag ctccgcatag gtgagcgtcc gctgctcgat
     1321 gagcgccggc cgccgcggaa agcgccgcac cagatcggca aacatcgacg ccaaggtttc
     1381 cggccgctcc gagaagcaga gcatgcggcg cgcgccgtag tgatcttcca gccgaagcgg
     1441 tccgcccaac ccctgggccg cccaatacgg atcctggact ctgctcacgc cgtcgcctcc
     1501 gcgacgcgcg cccggttggc gccgcgctcg atcagatact cgcgatcgac gagccgcgcc
     1561 gaatgattga ggtccgactg ggcaatgagc acgccgagct ccgtgccttt gagcgcggcg
     1621 atcacttcgg cgaggcgctg cgacagcgcc ggtgccaccc cttcgaaggg ctcgtcgagg
     1681 agcaggagcc gccgtcctgc catgagcgcc cgggccagcg ccaccagctt ctgctgtccg
     1741 ccgctcagga gcagcgcctt ccgcgcccgc atggcggcga cctcgggcat caggccatag
     1801 acgaaagcga gccgagcctt gccctcggga tcgcgcgtcg cccagctcgg caggaggata
     1861 ttctcctcga ccgagaggag cggcacgagc cggcgatcct cgggcatgta gccgatgccg
     1921 aggccggcgc ggccatgggt cggctgccgc gcgagatcga cgccggcgaa gcggatctcg
     1981 ccgtccgccg gcagcagccc catgaccgcg cgcaaggtcg tagtcttgcc ggcgccgttg
     2041 cgcccgacca atccgatcat ctcgccgggc gcgaccgcga ggctcaggcc gcgcagcacc
     2101 tcgaccgatt gtatggacac gcggagatcg accacggcca gtccgctcgt cattggacga
     2161 tccccgtgac atggcgctgc acgtcggcgt tgcccagcac cgccgccggc gcatcgtcgg
     2221 cgatcacgcg gccgtcatgg aaagcgagaa cgcgctcggc atagcgctcg acgatctcca
     2281 tgtcatgctc gacgaagagc accgtgacgt cggcgcccgc cagcgccgcc atgatcacgt
     2341 tcatcagcgg gaacttctcc tccgcggcga cgccgctggt gggctcgtcc agcagcagca
     2401 gcttgggccg ccgcgtgacc gccatggcga tgtcgacgag cttgcgcacg ccgcccggca
     2461 acgccgcgat cgcgtggccg cgatattcgg cgaggcgaaa ccgcgccagc aattcctcga
     2521 gctcggccac gagcgcgcgc gtccgtgccg gccgccaggc ggagaggccc tcgggtcgtc
     2581 cgatgcccag cgccatcaac agattctcct cgggcgtcag gctggcgaag agctgcggca
     2641 tctggaacga gcggctcacg ccatggcggc tcacccggcg cggcgcgagc ccggtgatgt
     2701 cgacgccgtc gagcaggatc gagccgcggt ctggcttgat atagccggtc accatgttga
     2761 cgaaggtcgt cttgcccgcg ccgttgttgc cgatgaggcc gacgcgctcg ccgcgccgcg
     2821 ccgcgaccga cacgtccttg gcggcgatca ccgcgccgaa ggccttgtgc aagcccttgg
     2881 cctcgatgag cgcggtcatc tgaggctctc gagcggacgc gtcaccgatt cggcggccgc
     2941 gccgcggcgc cggcgcaaca acgagccgag cccctccggc aggaacagaa tgacgagcag
     3001 cagaaagagg ccgagcgcca tctgccagct gttgggaaaa tactggctcg aaaaagagcg
     3061 tacgagctcg agcaggatcg acgccaggaa gacggcgacg acactctgat ggccggcgag
     3121 gatggcgagg aacacgaact cgcccgagct ggtccagaaa gcgaaatccg gatcgacatg
     3181 gccaagggcg agcgccgcca gcgcgccgcc gacaccgccc agcgccgccg cggcgacgaa
     3241 attgccgaac gccacacgcc ggaccgaggc gcccagatat tcgacgcgga tctcgttgtc
     3301 gcgcgcggcg agcgacagca atccgcgcat cgaatcgaaa tggacgcggc agagcaccgc
     3361 cgccgccacg gtcgacgcgg cactcaacag aaacaacgca tagccgagcg cctgggatgt
     3421 ccccggccga taggagagaa agctcggcgc gtgaagattg atcccgtcgg agccgcccag
     3481 cgcataggtc ttgttgagaa cgccataggc catcatcgac agcgcgagcg tcagcgtcgc
     3541 aaagaagatg ctgcgatatc ccgccaaaag cggcgcgatg gcgaggccga gcagcagcgc
     3601 cgcgatgccg ccgattgcga cgagaatcag cgcgtcgccg gtccagcgca tggccagccc
     3661 cgccgcatag gcgccggcgc aatagaagag cccctggccg aaggagacga gcccgccccg
     3721 catcagcagc accaggccga gcgcgaccaa tcctttggcg aaggccatgg tcgccaggaa
     3781 cacgacccag ctcggggcgg cgcggccgag cagcgccagg agcgcgaagc tcgtgagcgc
     3841 gatggcgaaa ggcgcgaaac ggcgcatcag atcttccgca gcgctatgcg ctggaagatc
     3901 ccctcgggac gcagcgccag cacgccggcc atcacgagat agacgatgaa gagctcggcc
     3961 gagggcatga ggtggatcgc cgccgagcgc gcgaggccga cgagtagcgc cgcgatcgcc
     4021 gcgccctcga cgctccccag accgccgatg acgacgacgg cgaagctcaa gaggatgacg
     4081 gtgatgctga tgcccggctg gaccgagatc ttgggcgcgg tgaacgcgcc gcccagcgcc
     4141 gccaggaaga cgccgacggt gaaggcgaga acgaacaccc gcgtgacgtt gacgcccatg
     4201 gcgcggctca tctcgcggtc gtggatgacg gcgagtacga tcttgcccgt gatcgtccga
     4261 ttgaggccga gccacaacag cacgccggtg atccccgcga gcgcgaccag cagcaggtca
     4321 tagccgacat aagtgaggcc gagcagattg acgttgccga ggaggccata gggctcatag
     4381 gcgtaatagg gattgacgcc ccagatcagc gtggtggcgt cctcgagaat gaggaagagg
     4441 gcataggtca ccaggaccag cacgatctcg tcgcggcgat agaacaggcg aaggatgccg
     4501 cgctcgagca gcggtcccac caccacggcg acgagcagcg ccgcccccag catgacgata
     4561 tagccgagga gcggcggccc gcttcccgaa taataccagc cgaccgcgct cgccgcggta
     4621 taggcgccga tggcgtagag gttgccatgg gcgatgttga gaatgttgag cacgccaaag
     4681 acgagcgtca gcccgaccgc gacgatgaac agccaggacg cgtcgatcac cccgtcgatg
     4741 acgatggtcg cggcaagttc cacggggcgg gcttgccgct atgggcaaag ggacaagtgg
     4801 tcactcgcac tgcgccccgg ggaagccttt gctgatccac tcctccgcct tcatgttggc
     4861 gggcggattg acgcattccg cgatgaagtg ctcgatgtcc accagcttca ccatctttgc
     4921 ctgggcatcc cattgggtcc gcccgaccgc gttcggctgg atcgcctgat ggccgtcgcc
     4981 cagtgccatt ttgatatggc ctgagggcgc atcccattcg agcccggtca tcgccgcggc
     5041 cagttcctcg gtcgagggct tcttgccgcc attggcggcc attgccgtct cgaccgcggt
     5101 cttgatgccc agcagcgcct gaatcatgcg gtattgcggc tgcacgggat aggtcccggg
     5161 ataggctttc ttgtaagcgt cgacgaacca gtcgttgagc gggcttcgct cggcgaattg
     5221 gccgttggcg ccgcgcgcgc cgatgatcac gccgtccggc atcttgtcgc cgagcggctg
     5281 cagcacatgg tcggacgcgc tcaggacgac gtagcggtcc ttgaagaggc cgcgcggcac
     5341 cgcctgaagc accaaggcct ggagatcgcc gccccagagg ctcgaataga cgaggctggg
     5401 cttctcctgc agcagggtcg agatctcggt gccatattgg cccgcgccga atttcggcca
     5461 gagctcggcc gtggctttgg catcggggtc gagctgcgcc atgctcgcct tgaaatcctt
     5521 ccaggaatcc tggccccagg cataatcctg gttgatggcg cccatatcgc cgatattgag
     5581 cttgcgcttg gcgatgtagc gggccatcgc gacattatcc atcgcgccat gggccgcggt
     5641 gcggaagacg tagtggaact tgttctcttc gaagatgcgg ggcgtgccgc aatcgtagag
     5701 gatcagcagc ttcttgagct cctcggcggc aggcgacacc gccaggcaat cgccggagcc
     5761 gacatagccg acgatggcgt cgacctggtc gcgctcatag gcgttgcgca gctcctgcac
     5821 ctgcttggtg gcgccgccat tctcatcgat caccaccgcc tcgatcttga ggccgccgaa
     5881 gccgggcttg tcatagggct tgggcaggac gccccccttg ttcagggcgt cgatgaccat
     5941 cttgccgctg ttccaggccg gcacgccgaa ggattcggcc gcctgtcccg acaggaaggt
     6001 gaccaccccg agcttgaagc tgttctcgtc cgcggcagcc tctcccgccg tcatcgcggc
     6061 cgccgccgca gcgatcgcgg cgaagccaag catgcttttg cgatagctca tatcggtcct
     6121 cccggtacca ttcttgttcg ctcggaccac cccgccctcg gcccggcctg ctcctgaaac
     6181 tgtgcctgaa ttcccccgtg gagtccacgt cgccgcccgc cgcgacacgc gggaagggag
     6241 gccgatgcgt gccgatcccc tgagcgccgc gccgctaaac gaaggtgtat gcgtcaccat
     6301 accgtcgtga gatggcggcg aaacgcaggt gcaatggagg cgctctggcg ccgccgctag
     6361 cgtcgcttgg cttccgttac attcgtgctg ccgccgcaac cgcgacggga cgatgcggcg
     6421 ccaaggaacg acttggtgtt ctcagcttca tggatggccg cgccgtccgg cgcgacccga
     6481 cacgactcaa ggaaggggtt cgatccatgg cgagaagcgt cgctgaccaa atcgcggaaa
     6541 ctctggctct cgccggcgtc aagcggatct acgggatcgt cggcgacagt ctgaacggcc
     6601 tgaccgacgc cattcgccgc caggccaaga tcgagtggct gcatgtccgt cacgaggagg
     6661 tcgccgcttt cgccgccggt gccgaggcgc atctgacggg cgatctcgcc gtctgcgctg
     6721 ggagctgcgg accgggcaac ctccatctca tcaacggcct cttcgattgc caccggtccc
     6781 gcgtgcctgt gctcgccatc gctgcgcaga tcccctcgcc cgagatcggc agcggctatt
     6841 tccaggaaac ccatccgcaa agcctgtttc aggagtgcag ccactattgc gagctcgtct
     6901 ctagcgtgaa ccagatgccg cgcgtgctcg aggtggcgat ccgcgaggcc gtcggcaagc
     6961 gcggcgtgtc ggtgatcgtg atccccggcg acgtggcgct gcaggcggcg ggcgacgcgc
     7021 cagcggcaaa gctcggcggc cttttgccgc cacgaccggt cgtcaccccc gacccccggg
     7081 atctcgaccg cctggcagcg ctgctcaacg gcgacggccg tgtcactctg ctctgcggcg
     7141 cgggttgcca aggcgcgcat gaccaactcc tggcgctagg cgagcggctc aaagcgccca
     7201 tggtccacgc catgcgcggg aaggaacatg tcgagtggga aaacccctac gacgtcggca
     7261 tgaccgggct gatcggcttc tcctcaggct actacgcgat gctcgactgc gatgtgctgt
     7321 tgatgctggg gaccgacttt ccgtaccggc aattctatcc ggagagaccc ggcgtgcgga
     7381 tcgcgcaagt ggacctccgt gccgagaaca tcgggcggcg cgcgccggtc gatctcggcg
     7441 tcgtcggcga cgtccgcgcg acgatcgccg cgttgctgcc gctgctgcag cagaagcgcg
     7501 acgccacgca tctggcgcag gcgcgcgagc attacgccaa ggcgcgcaag gagctcgaca
     7561 gattggcgat gggcacgccg ggcaagcggc tgatccaccc gcagcagatc gccaaggcgg
     7621 tgagcgatca ggcggcgggc gatgccattt tcgcctgcga tgtcggcctt ccgacggtgt
     7681 ggtctgctcg ttatctcgcc atgaatggca agcggcggct gctcggctcc ttcttgcacg
     7741 gctcgatggc caacgcgatg gcccaggcga tcggcgccca ggcagccttt cccggccgcc
     7801 aggtggtctc gctctcgggc gacggcggct tcaccatgct gatgggcgat ttcctcagcc
     7861 tcaagcagct cagtctgcca gtcaaggtcg tcgtcttcaa caacggcgcg ctcggcttcg
     7921 tcgagctcga gcagaaatcg accggcttcc tggatttcgg cacggagctg caaaatccca
     7981 acttcgcggc catggccgaa gccgtcggcg tgcgcggcat ccggatcgag gacgcggccg
     8041 atgtcgaggc cggcatcgcc gccgcctttg cgcatgacgg gcccgtgctg gtcgatgcgg
     8101 tcgtcaaccg gaccgagctc gccatgccgc cctcgatcac cgtcgagatg gcgaagggct
     8161 tcacgctcta catggtgaaa gccgtcttca acggccgcgc cgacgagatc atcgatctcg
     8221 cgaaaaccaa tctctggcgc tagcctcgcg tggacgttcg cgtctactct aaccaagcta
     8281 aatatcccct ctcccgcatc gcgggagagg gagggaccca ctgcgaagct tcagcgaagc
     8341 agtgggaggg tgaggggacg tttcaagcaa agctgctttt gcctgaagcg tcccctcacc
     8401 cggcgcgggc tgcgcccgcg ccaccctctc ccgcaatgcg ggagagggga ggaatgcgca
     8461 tcattgagaa tgtccgcgcg gcctaacccg cggcgggctc acccgttgcg gggacggtca
     8521 gctcgcttcg agatggctgt atcggacgcg gttgagattg ccgtgctcga cggcccaggt
     8581 ttcgctgaac acgacggtgc cgcggaggtc gacatattgc accgtcagca gcgccccctc
     8641 gaaggtgagg cgggcgaagc cgttgaaacc gacggtcagg ttctcgtcat tcggatagag
     8701 ccgcttgtcg gtgaactcga cgccgaattc gggatggagc ggtttcgcgg gcggcaaatc
     8761 cacgggcatg ccaccatggc cgatgcaccg gccgaaggtg cgaaggccgc cgggcttgtc
     8821 gaactcctcg tagatcgcga gacggtgctc atgcccccag aaccacagca cggggccttt
     8881 gacgaacgcc gccgcttggc gcgccggcgt cggatagcaa tagtcgaagc aggagaaata
     8941 gggatggtgg ctcaagatga cgagaccgcg cggatcgtcg gcgcgtgggc gcacgacctc
     9001 gcgcagccat tcgaccgtct ctggcggcaa ggcggaactg ggctgacgga agtattcgat
     9061 gatcgggatg ccgatcgagt tgtacccggt gtcgagggcg atgacgcgcc aatgctcgtt
     9121 ctcgagacag aagaagctcg cgcgctgacc ctgtgccttg ccgtcgcgga cgagacccat
     9181 cgccggcagg atgccatcga aataaccgta tcccaacgca tacatctcgt gattggcgtt
     9241 gagcgcgaag gtgccgcgcg agccgctggg ccagcggcag gggacgtagt gcgtcgcggg
     9301 attgcggatg ccgaggaaat tcgcattcac ctcggtgggt gtgccgacat agtagacgtc
     9361 gcccaggtga atcgcgtaat gcggcttgaa gtccgcgatc cgatttgcga cggcggcggc
     9421 ctcgtcggtc ccggtccccc aatcggcggc gagcgccacg cgaatctcgc ctccgtcgcc
     9481 gtcgagcgga tagatgccct gatcgctgtc gcctttcgca taggtcggaa acgggtggcg
     9541 tcgtccgatc cggtgcccga gatactggca gaaccaggtc cagatgcgtg acggcgagaa
     9601 cgccgccatc gacggaaacg gtgagggccg ccgagcgcga aggcctttga tccgcggcgc
     9661 cggcggcggc gcctgtttgc gcaaataggt caggatcggg ctgtgcgaaa gcttgcgcag
     9721 atcctgcaga tccctgtggc ccgaaggcac ggctggcagt gtcatgatct tgtgcgctcg
     9781 cccccttccc ccgtggcacg agtcgaccga cgaatcatgg caggatggga acgcgccgac
     9841 aagcgctgcg cgcgccggaa atcgtcgcga tgtgacgtag ttcacagccg acgttgcggc
     9901 ggatgcgcgt ggaaggacgc agcgggaatt cgcagactgc gacggtatgg tcgcggcccc
     9961 gccgtgtgac ctcgccgcac ccgcggccgt ttccctcgtc aggcaccttg ggctatggtc
    10021 gcgcctgccg ttgttgaacc accgaacctc tcgggtggct cgggtgtcga attccttccg
    10081 gctcatcgcg accttgaccc tggcgctcac cgtcgccgcg ggcgacgctg tggcgcaggc
    10141 cgagaccgcg gcgctctctt cgagcgcgga gcagctttat ggcgcggcgc ggccgaagct
    10201 gctgcagatc cgcacgctgc tcgtcgccac ggggcggcag tcctcgatcg gttcggggtt
    10261 tctcgccggc gaggacgcgc tcgccatcac caactaccac gtggtctcgc aagcggcgct
    10321 cgagcccgat acctaccgcc tcgaatatgt cggggcggac ggccataagg gcgaactcaa
    10381 gctgctcgcc atcgacctgc cgaacgatct ggcgctggtg cgtctcgacc agagcggcgc
    10441 cgggttcttc gagctcggcg acacggccgg cgagccgccc aaaggcgagc gtctctattc
    10501 catgggcgac ccgctcgatc tcggcttcac catcgtcgaa ggcacctata gcgggctcgt
    10561 cgagcgcagc tacaacgagc gcatccactt cacgggcgcc cttaatcccg gcatgagcgg
    10621 cggccccgcg gtcaccggcg acggccgcgt cgccggggtc aacgtcgcca agcagcaggg
    10681 cggcgagctc gtgagcttcc tcgtgccggt tcggtttgcc gcggcgctgc tcgcgcgcgc
    10741 ccgcggcgcc gagccgctcg agcccgcggc cttccacgcc gagatcggcc gccagctcgc
    10801 ggcgtggcag gcgcggcttt acgcgagctt cgccgagcgc ggctttcgcg cggccggctt
    10861 cggcccttac caggcggccg agagcaccgc gccctggttc acctgctggt cccagaccaa
    10921 tgccggccag gtgccgaagc cgcgggccag cgtcgacgtc accaactgca acagcgacac
    10981 gcagctcttc atcgccggcg acctcaccac cggtctcatc catatcggcc attccttcgt
    11041 gcgcgcgctc gatctcaacg cgttccaatt cgccgccttc ctgacgcagc aggcgcagac
    11101 gccctggctc ggcggcttcg gccggaaatg gctgacgcag cggcgctgtc atgaggattt
    11161 cgtcacggcg acgccggaag gcgagcatcc ggcgctgcgc gcggtgtggt gcgcccgggc
    11221 ttatcgcgaa ttcgccgggc tctacgacgt gtcggtcacg gccgtgaccg aggatcgcgg
    11281 cagcgaggcg ctggtctcgc ggctcacgct gcaggcgatc gattacgacg acgccctgaa
    11341 gatcacccgg ctcttcctcg aagacatgca atggaccagg tgatctgggt cgagatcctg
    11401 tcgcgccatc gcgaggtcgt cgcccgccac cgcttcgctg ccgccgagct gcatatcggg
    11461 cgaggctatg acaacgatct cgtgctcgac gatccgcatg tcgccgtcaa ccacatccgc
    11521 atctatcgcg gcgaagccgg cgatctcatg gccgaggaca tcggcagcgc caacggcctg
    11581 ttcatcgacc gcgaccagcg ccggtacgag cgcgtcgccc tcgacggcga ccgcgtcatc
    11641 cgcatcggcg gcgtgcagct gcgcgtgcgc gagcgcggct acgccgtgcc acgcgagcgc
    11701 gtgcagcatc cggagcggcg gctctggccg gtggcgctcg ctctggcgct ggccatcctc
    11761 ggcatccagg cgctgggcct ctggctcgcc gagacgggcg aggtaagggc gtcgcgctac
    11821 gtcgtgccgt tgctctcgat cgccggcccg gcgctcgtct ggaccgcggt ctggacggtg
    11881 ctgtcgcgcg tcttcgcggg acgggcggcc ttcgagcgca acctcatcac cgcgctcgcg
    11941 gggctcctcg cctactcgct ctatgacgag ttcgcccagt tcgccgcctt cggcctctcc
    12001 tggcgcctgc cgatcgctta tgagtatgtg gcgatgtggg ccgtcgtcgc gctcgtctgc
    12061 tatcgccaca tcgagacgct gggcgcgcgc cggcgcctcg tcgcggccgc cggcgccggc
    12121 gtcgcgctgc tcggcatcgc cacgcaatcg ctgacgcagt cggaggcacg c
//
LOCUS       DASYNS010000249         7035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1775237, whole genome shotgun sequence.
ACCESSION   DASYNS010000249 DASYNS010000000
VERSION     DASYNS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775237"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VGP50_07845"
     CDS             complement(<1..357)
                     /locus_tag="VGP50_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein"
                     /protein_id="HEV8027329.1"
                     /translation="MSAGGREGLAALRHRLEALEREGRHPRAVLPFGVPAIDGALPGG
                     GLALGALHEVAGDGGDGEDGAGAAAFLAGILARLRPPRPVLWCLPAADLHGPGLAACG
                     LAPERLILAQPREDQVL"
     gene            complement(453..1040)
                     /locus_tag="VGP50_07850"
     CDS             complement(453..1040)
                     /locus_tag="VGP50_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027330.1"
                     /translation="MRRNLRPLLALLLLVLAGCSTTAVSLDYTRATAPLAGAQPQVAV
                     GSFADQRRDNDPRQLGAIRGGMGQPLKTLVLSEDASVVVQKAFAQALDKRGLLAPPGS
                     GRYTLSGVINKLDCSQYIRREAHADIVVSLTDNATGKVVLQQTAHADGVTEPSGADYS
                     IFGKVEDLRVVAERTLQDAIDKALSDARFAQAVAG"
     gene            complement(1149..2753)
                     /locus_tag="VGP50_07855"
     CDS             complement(1149..2753)
                     /locus_tag="VGP50_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEV8027331.1"
                     /translation="MIQTIETLVLLLVVIAAVAVVAARSKIPPAILLVLTGVILALVP
                     GLPSLELAPELVLLLVLPPLVYDSAVAMSWREFRFNLRPISLLAVGSVVFTTVTVAAA
                     THWLLAFPWPIGFLLGAIVSPPDAVAPLSIARRMQIPRRILVVLEGEGLANDATALIF
                     YRFALAAASTGLFSLGQAVGTFAAIVVGEILWGIGVGWLMLRLRHWVRDPRIELTLSM
                     VTPFLAYWPPEHLGGSGVLATVTTGLYISWNGLRLISAATRLQGIFFWGFFIYLVEGM
                     VFLITGLQARTLIARIGTYPATELAISAVIVAAVVIVARFVWTYPAIYVPRWLLPRLK
                     RRDPSPPWQWAFALSFTGIRGIVSLAAALAIPFVTAAGQPFPDRDLILFLTFSVILVT
                     LVGQGLMLPAVIRALGLANAGRRERAHERAEEQAARRAAIATALERLEQLAGERHLPD
                     EVVRPLRARHRERLRHAEQRSDGDDGHRKLTELHEEIEGLLIDAERQHVNDLFRDGGL
                     NDEARRRIERELDLREASLANRREEE"
     gene            2940..3554
                     /locus_tag="VGP50_07860"
     CDS             2940..3554
                     /locus_tag="VGP50_07860"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEV8027332.1"
                     /translation="MLPGFFGEVQRVVGDYGYLAVALGILLEDFGLPTPGETMLITGA
                     ILASGGALNIYILLPLAWLGAVIGDNIGYVIGYYGGHRLMLRHGARIGITEERLQYVE
                     RFFERYGGWVIVFARFVIVLRQFNGIVAGTLEMHWLHFVVLNAIGAALWVGFWGMLAY
                     WLGKGVLVYVADIQRLAPVMLALVAAALLGGAAYLWWRYRRRGR"
     gene            3600..4025
                     /locus_tag="VGP50_07865"
     CDS             3600..4025
                     /locus_tag="VGP50_07865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV8027333.1"
                     /translation="MPASIFDTMKAPPTAQLLGWTLLELDVEKKSIKVGFDGKADFLN
                     PTGHVQGGILSAMLDDAMGPIVVAATGGAAFPTTIDLHVHFIRPVKPGAISVTAEITN
                     IGKQLVFLEGRLFDSAGKLCARATAAALLSALDGIRKSR"
     gene            complement(3991..4260)
                     /locus_tag="VGP50_07870"
     CDS             complement(3991..4260)
                     /locus_tag="VGP50_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027334.1"
                     /translation="MSSSIRFAIRGRAPLLLALLASCATPSALDAVTADSDEIVFSYT
                     ADRAAEAERQASLYCANLGRAARLRGETRGASDRLIATFECRPAR"
     gene            4335..5258
                     /locus_tag="VGP50_07875"
     CDS             4335..5258
                     /locus_tag="VGP50_07875"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV8027335.1"
                     /translation="MRIAIVGQQAFGKAVLEAFLARGDVVAGVFVAPEPPGSRPDPLH
                     AAAVEKGVPVFLTAHYGAPEAIESLRRLAVDLGIMAYVTAFVPERFCRVPRLGTIQFH
                     PSLLPQHRGPSSINWPIILGRRRTGLSIFRPVKGLDEGAVLLQKEVDILPDDTLGSVY
                     FDKIFPLGVAALLEAADLVAAGKAREIAQDESQATYESWVREAEARIDWGHHVETIYN
                     LIRGCNPAPGAWTTLGGRRFYLFDAKKHVARTFAAVKGHRSGEVVAATAASIAIHAPG
                     GSIELLRCRFDDGKKIAAGEAGIAVGDILGA"
     gene            complement(5574..6896)
                     /locus_tag="VGP50_07880"
     CDS             complement(5574..6896)
                     /locus_tag="VGP50_07880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV8027336.1"
                     /translation="MSDASIALPEESTDVLVVGGGPAGSTIAALLAERGEQVVLVEKE
                     KHPRFHIGESLLPFNLPLLERLGVKQAVEKIGMPKYGAEFFSTRLGKTSVYAFADAWD
                     KGFPFAYQVRRSEFDHLLLENAAAKGAAVVEECRITEVDFPPQGGAVATGRDAEGRVR
                     RWRAKFFVDASGRDALLASRFGIKRRNPRNNSAAIFGHFTGAHRSPGKAEGNIGIFWF
                     DHGWFWFIPLADGTTSVGAVCRPAYLKSRKTDMTAFFMATIALCPPLAQRLEDARLVL
                     PATGTGNYSYRSKRMMGQSYIMVGDAYAFIDPMFSTGVYVAMSSAFLGADVVSACLRE
                     PQKSRRAFKTFDAKIRHALHAYSWYIYRINMPALRALLSTTENPFRLQEAFLSVMAGD
                     VFRPSPVHARLIVFKAIYFLSSLRRFKSSFLAWSKRRSDARASTREAT"
BASE COUNT         1228 a         2376 c         2335 g         1096 t
ORIGIN      
        1 gagcacctgg tcctcgcgcg gctgcgccag gatcagccgc tcgggcgcga ggccgcaggc
       61 ggcaaggccg ggaccgtgga gatcggcggc gggcaggcac cacagcaccg gccgcggcgg
      121 ccggagccgc gccaggatgc ccgcgaggaa agcggcagcg ccggcgccat cctccccatc
      181 ccctccgtcg cccgcgacct catggagcgc gcccagggcc aagccgccgc cgggcagcgc
      241 cccgtcgatg gccggcacgc caaagggcag gaccgcccgc ggatgccgcc cctctctctc
      301 cagcgcctcg agccgatgcc tcagcgccgc caggccttcc cttccgccag cggacatggc
      361 ttcccattca acgccaccca ttcaacacat ggggttcttg ttttgttccg aaaaaggagg
      421 gctgtcaagc cgagcgcgag acgacaatgc ctctagccgg ccactgcctg cgcgaagcgc
      481 gcgtcggaca aagccttgtc gatggcgtcc tgcaatgtcc gttccgccac cacgcgcaga
      541 tcctccacct taccaaagat ggagtaatcc gcccccgacg gctccgtgac cccgtctgca
      601 tgggcggtct gctgcagcac caccttgccg gttgcattgt cggtcaagct cacgacgatg
      661 tcggcatggg cttcccggcg aatatactgg ctgcaatcga gcttgttgat gacgccgctg
      721 agcgtgtagc gcccgcttcc cggcggcgcc agaaggccgc gcttgtcgag tgcttgggcg
      781 aaggccttct gcacgacgac gctcgcgtcc tccgagagca cgagcgtctt cagcggctgg
      841 cccatgccgc cgcgaatggc gccgagctgg cgcggatcgt tgtcgcggcg ctgatcggcg
      901 aagctgccga ccgccacttg cggctgcgct cccgccagcg gcgccgtcgc gcgggtgtag
      961 tccagcgaca cggccgtggt cgagcagccg gcgagcacga gaaggagcaa ggccaggagc
     1021 ggccggagat ttctgcgcat gttctgctgt tgcctcggtt tgctttgcgt tgcggggatg
     1081 cccgcgatgg cctgaaatct agcagaccgc gacgaagcgc tccacgagca acgcagctac
     1141 gatttccctc actcctcttc gcgccgattg gcgagactcg cttcgcgcag gtcgagctct
     1201 ctctcgattc ggcgccgcgc ctcgtcgttc aatccgccgt cgcgaaaaag atcgttcaca
     1261 tgctgccgct cggcatcgat cagcaggcct tcgatctcct cgtgaagctc ggtcagcttc
     1321 cggtggccgt catcgccgtc gctccgctgc tcggcatggc gcagccgctc gcggtggcgc
     1381 gcgcggagcg gccggacgac ctcgtccggg agatggcgtt ccccggcgag ttgctccagc
     1441 cgctcgagcg ccgtcgctat ggccgcgcgc cgcgccgcct gctcctcggc tctttcatgc
     1501 gcgcgctcgc gccgcccggc attggccagt cccagcgcac ggatcacggc gggcagcatc
     1561 aggccctggc ccaccagcgt caccagaatg acggagaagg tgaggaagag aatgaggtcg
     1621 cggtcgggaa agggctggcc ggcagccgtg acaaagggaa tcgccaacgc cgcggccagc
     1681 gagacgatgc cgcgaatgcc ggtgaacgac aatgcgaagg cccattgcca gggcggcgac
     1741 ggatcgcggc gcttcagtcg aggcaagagc cagcgtggca catagatcgc cggataggtc
     1801 cagacgaagc gcgcgacgat gaccaccgcg gcgacgatca cggccgagat cgcgagctcg
     1861 gtcgccggat aagtcccgat ccgggcgatc agcgtgcgcg cctgcaggcc cgtaatgagg
     1921 aaaaccatgc cctcgaccag gtagatgaag aaaccccaga agaagatccc ctggaggcgt
     1981 gtcgccgcgc tgatcagccg cagcccgttc caactgatgt agagaccggt ggtcacggtc
     2041 gcgagcacgc ccgagccgcc cagatgctcg ggcggccaat aggcgaggaa cggcgtcacc
     2101 atcgaaagcg tgagctcgat gcgtgggtcg cgaacccaat gcctgaggcg cagcatcagc
     2161 cagccgacgc cgatgcccca caagatttcg ccgacgacga tggcggcgaa agtcccgacc
     2221 gcctgaccga gcgagaacag gcccgtgctc gccgcggcga gcgcgaagcg atagaaaatg
     2281 agtgccgtgg cgtcgttcgc cagcccctcg ccttcgagca cgacgaggat gcgccgcggg
     2341 atctgcatcc ggcgcgcgat cgagagcggc gccaccgcgt cgggcggcga gacgatggcg
     2401 cccagcagga agccgatggg ccaagggaac gccaggagcc aatgggtcgc cgccgccacg
     2461 gtcacggtcg tgaacacgac gctgccgacg gcgagcagcg aaatcgggcg gagattgaag
     2521 cgaaactcgc gccagctcat cgccacggcg gaatcataga cgagcggcgg cagcaccagg
     2581 agcagcacca gctccggcgc cagctccaac gagggcagcc cggggacgag cgccaggatc
     2641 acgcccgtca gcacgagcag aatggccggc gggatcttcg accgcgcggc gaccaccgcg
     2701 acggcggcga tcacgacgag cagcaggacc agcgtctcaa tcgtctggat catcgagcat
     2761 accccctcgg cgatgatcgc cgctgatgcg ctcgagtcaa cttgggacgg ccggtgcgga
     2821 cggatcgcgg tcgcgattcg gctcctccgt cgattgaccg ctttccgctc tcaagcaaag
     2881 atggcgcgat ccacgagggg gaggacgccg tggcgcgaag tgcactgacc gtcgattaag
     2941 tgcttcccgg attcttcggc gaggtgcagc gcgtcgtcgg cgactacggc tatctcgccg
     3001 tcgcgctcgg gatcctgctc gaggatttcg gcctgccgac gccgggcgag acgatgctca
     3061 tcaccggggc gatcctcgct tcgggcggcg cgctgaacat ctacatcctg ctgccgctcg
     3121 cctggctcgg cgccgtcatc ggcgacaaca tcggctatgt catcggctat tacggcggcc
     3181 atcgcctgat gctgcgccat ggcgcgcgca tcggcatcac cgaggagcgc ttgcagtatg
     3241 tcgagcgatt cttcgagcgt tatggcggat gggtcatcgt cttcgcgcgc ttcgtcatcg
     3301 tgctgcggca gttcaacggc atcgtcgccg gcacgctcga aatgcactgg ctgcattttg
     3361 tcgtgctgaa cgccatcggt gccgcgctct gggtcgggtt ctggggcatg ctcgcgtatt
     3421 ggctcggcaa gggcgtcctc gtctatgtcg cggacatcca acggctggcg ccggtcatgc
     3481 tcgcgctcgt cgcggccgcg ttgctcggag gtgccgctta tctctggtgg cgctatcgcc
     3541 ggcgcgggcg ctagaacgcc acggcgatcg tgcgtcctca ttttggcgag gagaatgcga
     3601 tgccggcctc gatcttcgac accatgaagg cgccgcccac ggcgcagctc ctggggtgga
     3661 cgctgctcga gctcgacgtc gagaagaaga gcatcaaggt cggtttcgac ggcaaggcgg
     3721 acttcctcaa ccccaccggc catgttcaag gcggaatcct gtcggcgatg ctcgacgacg
     3781 ccatggggcc gatcgtcgtc gcggctacag gcggtgctgc gtttccgacg accatcgacc
     3841 tccatgtgca tttcatccgt cccgtgaagc cgggagcgat ctcggtgacc gccgagatca
     3901 ccaacatcgg aaagcagctc gtcttcctcg aaggccggct cttcgattcc gccggcaaac
     3961 tatgcgcccg ggccaccgcc gcggcgctgc tcagcgcgct ggacggcatt cgaaagtcgc
     4021 gatgaggcga tcgctcgcgc cgcgcgtctc gccgcgcaag cgggcggcac ggccgagatt
     4081 ggcgcagtag agcgacgcct ggcgctcggc ctcggcagcg cgatcggccg tataggagaa
     4141 gacgatctcg tcgctatcgg cggtcacggc gtccagcgcg gagggcgtcg cgcaggacgc
     4201 gaggagcgcc agcagcagcg gcgcgcgccc gcgtatggca aagcggatgc tcgagctcat
     4261 gggccggggt tggcggcacc cgacgggtct gctacccatt cgcctcgcgc gaagaggggg
     4321 agcaagagac caacatgcgc atcgcgatcg tcggtcagca ggccttcggc aaggccgtgc
     4381 tcgaggcgtt tctcgcccgc ggcgacgtcg tcgccggcgt cttcgtcgcg cccgagcccc
     4441 cgggcagccg gcccgatccg ctccacgccg ccgccgtcga gaagggcgtg ccggtgttcc
     4501 tgacggcgca ttacggcgcg cccgaggcga tcgaatcgct gcgccggctg gcggtcgatc
     4561 tcggcatcat ggcctatgtg acggccttcg tgcccgagcg cttctgccgg gtgccgcggc
     4621 tcggcacgat ccagtttcac ccctcgctgc tgccgcagca tcgcggcccc tcctccatca
     4681 attggccgat catcctgggg cgccgccgca ccggcctcag catcttccgc ccagtgaagg
     4741 gactcgacga aggtgccgtc ctcctgcaga aggaagtcga catcctcccc gacgacacgc
     4801 tgggcagcgt ctatttcgac aagatctttc cgctcggcgt ggcggcgctg ctcgaggccg
     4861 ccgatcttgt cgccgcgggc aaggcgcgcg aaatagcgca ggacgaaagc caggcgacct
     4921 acgaaagctg ggtgcgcgaa gcggaagcgc gcatcgactg gggccatcat gtcgagacca
     4981 tctacaatct gatccgcggc tgcaacccgg cgcccggcgc ctggacgacg ctcggcggcc
     5041 gccggttcta cctcttcgac gcgaagaagc atgtcgcccg caccttcgcc gcggtgaagg
     5101 gccatcgctc cggcgaggtc gtcgcggcga cggcggcgag catcgccatc cacgcgcccg
     5161 gcggctccat cgagctgctg cgctgccgct tcgatgacgg caagaagatc gccgcgggcg
     5221 aggccggcat cgccgtcggc gacattctcg gcgcgtaagg ggctcaaggc ggccttcgtc
     5281 agcggtttag aaaagatcga gggcccgacg gatgacatcc cacctttcgg aagcggccgc
     5341 gtcgtcatac cctttccgtg cgggctccgt tcgcagccga cgtccgagaa atatccagag
     5401 cgctaccaat atcggccagg gcagcacgac gagcgccccc gctggccaaa gttcgccgac
     5461 aggggcgcca ttctggatcc ccacgatgac gaacgcgccc gccataaaca gtactggtgt
     5521 cgctgccacc aaaagacacg acaagatgat caggatccag acaacggtcg tcttcacgtc
     5581 gcttctctcg tcgacgcccg ggcgtcgctt cgacgcttgc tccacgccag gaagctcgat
     5641 ttgaagcggc gcaggctgga aagaaaatag atcgctttga agacgatcag ccgcgcatgg
     5701 accggcgagg ggcgaaagac gtcccccgcc atgacggaca aaaacgcctc ttgcagccga
     5761 aacgggttct ccgtcgtcga cagcagcgcg cgcagcgccg gcatgttgat gcggtagatg
     5821 taccaggaat aggcgtggag cgcgtgccgg atcttggcat cgaaggtctt gaaggcccgc
     5881 cgtgatttct gcggctcgcg caagcaggcg ctcacgacgt cggcgccgag gaacgcgctg
     5941 ctcatggcga cgtagacgcc ggtggaaaac atcgggtcga tgaaggcata ggcatcgccc
     6001 accatgatgt agctctgtcc catcatgcgc ttcgagcgat aggaataatt gccggtcccc
     6061 gtcgccggca aaacgaggcg cgcgtcctcg agccgctggg cgagcggcgg acagagcgcg
     6121 atcgtcgcca tgaaaaaggc ggtcatgtcc gttttgcgcg acttgaggta agccgggcgg
     6181 cacacggcac cgacactggt cgtgccatcg gccagcggga tgaaccagaa ccagccgtgg
     6241 tcgaaccaga agatgccgat attgccctcg gccttgcccg gcgatcggtg cgcgccggtg
     6301 aaatggccga agatggccgc gctgttgttg cggggattgc ggcgcttgat gccgaagcgg
     6361 ctcgccagca gcgcatcgcg tcccgatgcg tcgacgaaga actttgcccg ccagcggcgc
     6421 acccgcccct ccgcgtcgcg cccggttgcc acggcgcctc cttgcggcgg gaaatcgacc
     6481 tcggtgatcc ggcattcctc gacgacggcc gcccctttgg cggccgcgtt ctcgagcagc
     6541 agatgatcga actcggagcg gcgcacctga taagcgaaag ggaagccctt gtcccacgca
     6601 tcggcaaacg cgtagaccga cgtcttgccc agacgggtcg agaagaattc ggcgccgtat
     6661 ttcggcatgc cgatcttctc gaccgcttgc ttgaccccca gccgctcgag gagcggcaag
     6721 ttgaagggca agagcgattc gccgatatgg aatcgcggat gcttctcctt ttcgacgagc
     6781 accacctgct cgccgcgctc ggcgagcaat gctgcgatgg tcgagccggc cgggccgccg
     6841 ccaacgacca ggacatcggt ggattcctcc ggtagagcga tggacgcgtc actcatgtcg
     6901 cacctccgac ccttggcaaa aatcgtcgcg atcgggcggt tcattcgaga aacgcgccga
     6961 tggcctcgag aaacgcgtcc ggctgctcga gcggcgggca atgcccgcag cccggcaaca
     7021 ggacgaagcg cgcgc
//
LOCUS       DASYNS010000250         3087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2867435, whole genome shotgun sequence.
ACCESSION   DASYNS010000250 DASYNS010000000
VERSION     DASYNS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2867435"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1604
                     /locus_tag="VGP50_07885"
                     /pseudo
     CDS             1..1604
                     /locus_tag="VGP50_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620398.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase"
     gene            1686..2213
                     /locus_tag="VGP50_07890"
     CDS             1686..2213
                     /locus_tag="VGP50_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV8027337.1"
                     /translation="MAQDIDLAALARGDKASWDAFVRRYAGLVIAAVRGVARDGTDFD
                     DLAQEVFLRLCKDGFRLIKTYDAARASLSTWLTIVARSTARDAMRRRQLPVTPLDAVP
                     EALLATDGRPPEKLRLPEGLLSPRQKLVLSMLYDRDMEVAEIAQTLGVDPQTVRSTHH
                     KAMLKLRSHFATEES"
     gene            2292..2798
                     /locus_tag="VGP50_07895"
     CDS             2292..2798
                     /locus_tag="VGP50_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027338.1"
                     /translation="MGEPQHSTEEAALWRRWRSAAGRDAPVALDPLLLAAYAENRLEA
                     AEIDRVEEWLAAHPAAVEDILAARRAAHSALPDSPPALVTRATALLHHGDGQAQVFAF
                     RRPVPRWRAVATWSCMAASMLIAGVVGFNLGSDVYASLAGASSGALGQELLDPSGFLT
                     NTDEDSAI"
     gene            2795..>3087
                     /locus_tag="VGP50_07900"
     CDS             2795..>3087
                     /locus_tag="VGP50_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027339.1"
                     /translation="MTSLAEVEAKSGIRNRLLWVAFALSLTLNAFVVAGLVWSQAANP
                     RPANFADRLVESGASLNLSPTQRDNFNQFAQTIQQRTKDMREANLPLFRQIWD"
BASE COUNT          529 a         1037 c         1001 g          520 t
ORIGIN      
        1 tgggcgagtt cgtgttccat gacatgttct agctgggcga ggacgatacg ccctatcgca
       61 agctctcggc cgactacgtc gagacggcca gctttgccgg cgagcggctc gtcaaggtcg
      121 atcggcgcgc gctgacgctg ctcgcgcagc aggccttcgt cgattgccag cacctgttgc
      181 gcccaggcca tcttgtgcag ctgcgccgga tccttgagga tccggaagcc tccgacaatg
      241 acaagttcgt cgccttcgac ctcctcaaga acgccaacat cgccgccggc aaggtgttgc
      301 cgatgtgcca ggacaccggc acggcgatcg tcatgggcaa gaagggccag cggatctgga
      361 ccggcggcgg cgatgccgga gcactcgccg ccggtatccg ccgcacctac accgagacca
      421 atctgcgcta cagccaggtg gcgccgctct cgatgtatga agaagtcaac accggcgaca
      481 atcttccggc gcagatcgag ctctatgccg aggagggcga cgcctacaaa ttcctgttca
      541 tcgccaaagg cggcggctcg gccaataaga gcttcctcta tcagcagaca aaggctgtcc
      601 tcaacccgaa gtcgctgctg aaattcctcg acgagaagat ccgcacgctc ggcaccgccg
      661 cctgtccgcc ctatcacctc gccatcgtcg tcggcggcac gtcggccgag ttcaacctca
      721 agaccgtcaa gctggcgagc tgccgctatc tcgacacgct gccccgcgcc ggcgacgcgc
      781 aaggacgggc attccgcgac cacgccctcg aggaggaggt tctggcgctc acccggacga
      841 tgggcatcgg cgcgcaattc ggcggcaaat atttctgcca cgacgtgcgg gtgatccgct
      901 tgccgcgcca cggcgccagc gtgcccatcg gcatcggcgt ctcctgcgcc gccgaccgcc
      961 agatcctcgg caagatcgat gccgacggcg tgttcctcga ggagctcgag accaaccctg
     1021 ccaagtacct ccccgacatc gcggagggga gccttggcgg cccggtggtg cggatcgacc
     1081 tgaagcggcc catggccgag atccgccgca ccctctcgca atatccgatc aagacgcggc
     1141 tcgcgctcag cgggccgctg atcgtcgcgc gcgacatcgc ccatgcccgg ctcaaggagc
     1201 agctcgaccg cggcgcggcg ctgccctcgt atatttgcga ccatgcgatc tactatgctg
     1261 gcccggccaa gacgcccacg ggctacgcct ccggctcgtt cgggccgacc acggccggac
     1321 gcatggattc ctatgtcgac cagttcatgg cggcgggcgg cagtttcgtc accctcgcca
     1381 agggcaatcg tggcgccgag gtgcgggctg cctgcaagaa gcatggcggc ttctatctcg
     1441 gctcgatcgg cgggccggcg gcgcggctcg cccaggactg catcaagaag gtcgatgttc
     1501 tggcgtatcc cgagctcggc atggaagcga tctggcgcat cgaagtggtg gattttccgg
     1561 cgttcatcgt catcgacgac aaaggcaacg acttcttcgc ctgaagcaag attgcggcac
     1621 cggggaagcg gaacgccggc ttccccgttg tgaccgcagc gtggcgagga tgaaagggtg
     1681 agtgcgtggc gcaggacatc gaccttgcgg cgctggcgcg gggcgacaaa gcgtcctggg
     1741 atgctttcgt gcgccgctat gccgggttgg tcattgccgc cgtccgcggc gtggcccgcg
     1801 acgggacgga cttcgacgac ctcgcccagg aagtgttcct gcgcctctgc aaggacggct
     1861 tccggctgat caagacctac gacgcggcgc gcgcatcgct ctcgacctgg ctcaccatcg
     1921 tggcgcgcag cacggcgcgc gatgccatgc ggcggcgcca gttgccggtc accccgctcg
     1981 acgccgtgcc ggaggcgctg ctcgccaccg acgggaggcc gcccgagaag ctgcgcttgc
     2041 ccgagggcct gctctcgccg cggcagaagc tcgtgttaag catgctctac gatcgcgaca
     2101 tggaagtggc cgagatcgcc caaactctgg gcgtcgaccc gcagacggtg cggagcaccc
     2161 atcacaaggc aatgctcaag ctgcgtagcc atttcgcgac cgaggaatcc tgatcagaag
     2221 tttaatgaaa gaagtatcgc gggggatgaa atcgctgtta gatgcctaaa agggagggag
     2281 gagacgagga gatgggtgaa ccgcagcaca gcacggagga ggcggccctt tggcgccgtt
     2341 ggcgctcggc cgccggccgg gacgcgccgg tcgcgctcga tccgctgctt ttggccgcgt
     2401 atgccgagaa ccgtctcgag gcggccgaga ttgaccgcgt cgaggaatgg ctcgctgccc
     2461 atcctgcggc ggtcgaggat atcctggccg cccggcgcgc cgcccactcg gcccttcccg
     2521 attcgccccc cgccctggtg actcgggcga cggcgctcct gcaccatggc gacgggcagg
     2581 cgcaggtatt cgccttccgg cgccccgttc cgcgctggcg tgccgtggca acctggagtt
     2641 gcatggcggc gagtatgttg attgcgggtg tcgtcggctt caacctcggc agcgacgtct
     2701 atgcgagcct tgccggagcg agctccggag cgctgggcca ggagctgctc gatccctccg
     2761 gtttcctcac caacaccgat gaggattcgg ccatatgacg agtctggccg aagtcgaagc
     2821 caagtccggg atccgcaatc ggctcctctg ggtggcgttc gcgctttcgc tcacgctcaa
     2881 tgcctttgtc gtggccggcc tcgtctggtc gcaagcggcg aatccgcgtc cggcaaattt
     2941 cgccgaccgc ctcgtcgaga gcggagcgtc gctcaatctc tcgccgacgc agcgcgacaa
     3001 cttcaaccag tttgctcaga ccatccagca gcgcaccaag gacatgcggg aggccaatct
     3061 gccgctgttc cggcagatct gggacga
//
LOCUS       DASYNS010000251         5152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2243804, whole genome shotgun sequence.
ACCESSION   DASYNS010000251 DASYNS010000000
VERSION     DASYNS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2243804"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..236
                     /locus_tag="VGP50_07905"
     CDS             36..236
                     /locus_tag="VGP50_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027340.1"
                     /translation="MDDGFRRSIRPDPQLLRQRARRLCALAKQIAESGNHLIASQLLR
                     VVGMLEGMALMIEGPVETGLPN"
     gene            269..925
                     /locus_tag="VGP50_07910"
     CDS             269..925
                     /locus_tag="VGP50_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027341.1"
                     /translation="MIRRAALLAVLLLAAAPHAGARAALSCPTPTPPAVAVSVEAGAP
                     AIDNTLTQPALQQVAGTMPHGDLALGYYRANLEVRWRTFTAWRSAGTEACHWIERVAI
                     EIALVDRKIYIIRERRPGSCPYDSVLGHERKHQAVDDAVVAEYRPLLVRAAERAVAGL
                     PPPTPDSIDRGDAVEARLTGTVETALQSIVKAMLAVRAARQAAVDTPAEYRRIGAACG
                     "
     gene            967..1281
                     /locus_tag="VGP50_07915"
     CDS             967..1281
                     /locus_tag="VGP50_07915"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase"
                     /protein_id="HEV8027342.1"
                     /translation="MRGHHDMGGLPAGPVERSEHDHALWEKRVDALMLLLSAPSRRLL
                     RVDELRRNIEALPPDAYDRMTYYERWISAIANTLLQRGVITADELGRKMAAVAARGKD
                     AP"
     gene            1278..1580
                     /locus_tag="VGP50_07920"
     CDS             1278..1580
                     /locus_tag="VGP50_07920"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEV8027343.1"
                     /translation="MSETATLAPRFAPGARVSVRRADPPGHLRTPWYIRGHVGVIERL
                     CGAFANPEELAYNRPGVPKQPLYRVRFRQREVWPDYRGSAEDKIEVEIYQHWLEPA"
     gene            1582..2202
                     /locus_tag="VGP50_07925"
     CDS             1582..2202
                     /locus_tag="VGP50_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEV8027344.1"
                     /translation="MTTHSHPHPHETLPEHDERLGYYQTMEIAVRELLIAKAVFTADD
                     MRRAVEAMDARTPAQGARVVARAWCDPAFKARLIEDGTAAVGELGIDMGETRLLVVEN
                     RPGVHNLVVCTLCSCYPRMLLGLPPDWYKSRAYRSRAVREPRAVLAEFGTVLPEDVAV
                     RVHDSTADMRYLVLPAQPPGTAGWSEERLAALVGRDAMIGVTRPVL"
     gene            2388..>5152
                     /locus_tag="VGP50_07930"
     CDS             2388..>5152
                     /locus_tag="VGP50_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEV8027345.1"
                     /translation="MAIRAEQQKDDAVESVVALAAKRLGGDRAGDVARFLRLFYSHVP
                     PADILERAPEDLYGAAVSLWRFAETRQPGRAKLRVINPRIEEQGWRSDRTVVEIVNDD
                     MPFLVDSVTAALAGEGFTVHLVIHPVLRVERDAEGRLAALLDSGGGNGNGNGAGGGLR
                     ESVMHVEVSEERDPARLDALAAFLQHVLDEVRAAVQDWMRMRERLRAIAAELAAQPPP
                     LVEAERREGLDFLAWLDDDNFTFLGFREYRFGGGQESGLDILPGSGLGILRDEGHTVF
                     DGLRHFATLPPDVRQFLREPRLLMITKSNRRSCVHRAVYMDAICIKSFDASGNAIGER
                     LFVGLFTSLAYSRSPRAIPLLRRKVETALKRSGFESQSHDGKALLHILDTYPRDELFQ
                     IGEDELFETVLGILHLQERQRIALFLRRDPFERFVSCLVYVPRERYDTALRERFVHIL
                     EDALAGKVDSFNAQLDESVLARIHFIVRTIPGRLAQIDVGALERRLAEAGRSWADRLA
                     EALAEAKGEGPSRALLRRYAEAFPTGYRERFPAAAAVYDIERLEELAQGAPIAVTLYR
                     PLEAESRELRFKIYRQGAPVPLSDVLPVLEHLGLRVIAEIPYAVAPMGEPAASLQDFS
                     LTVPSGEVDVARDRARFEEAFLRLWAGVMESDGLNRLVLAAGLDWRQVVMLRLYVKVL
                     RQAGSAYSQAYMEDALAGHAAIARQLAQLFERRFDPKRERGRDAETAKLVQASERALE
                     DVASLDEDRILRSMLLLVTKSLRTNYFQRDAGGEAKPYLSVKLASREIDLLPLPRPLY
                     EIYVLSPRVEGVHLRGGKVARGGIRWSDRKEDFRTEILGLMKAQMVKNAVIVPTGSKG
                     GFVVKRPPATRDALQAEGIECYKTLLRGLLDLTDNIVGAKIVPPRDVVRHDGDDPYLV
                     VAADKGTA"
BASE COUNT          755 a         1801 c         1754 g          842 t
ORIGIN      
        1 ttgatggttc tgctcgacca cgaatggagg tcacgatgga cgacggtttt cgccgatcca
       61 tccgtcccga tccgcagctg ctccggcaac gggcacgccg gctctgtgcg ctcgcgaagc
      121 agatcgccga gagcggcaat catctcattg cctcgcagct gctgcgcgtc gtcggcatgc
      181 tcgagggcat ggcgctgatg atcgaggggc ctgtcgagac cggcctgcca aactagccgc
      241 cgtacctgcc gccgctatag ttgcggcgat gattcgacgc gccgcccttc ttgctgttct
      301 gctcctcgcc gcggcaccgc atgccggcgc gcgcgccgcc ctttcctgtc cgacgccgac
      361 gccaccggcc gtcgcggtca gcgtcgaggc cggcgcgccg gccatcgaca atacgttgac
      421 ccagccggcg ctgcagcagg tcgccggcac gatgcctcac ggcgacctgg ctctcggcta
      481 ctatcgcgcc aacctcgagg tccgctggcg caccttcacc gcctggcgca gcgccgggac
      541 tgaagcgtgt cactggatcg agcgcgtcgc gatcgagatc gcgctcgtcg atcgcaagat
      601 ctacatcatc cgcgagcgcc ggcccggcag ctgtccttat gacagcgtgc tcggccacga
      661 gcgcaagcat caggccgtgg acgacgccgt ggttgcggag tatcggccgc tccttgtgcg
      721 cgcggccgag cgcgccgtcg ccgggctgcc gccgcccaca cccgactcca tcgaccgggg
      781 cgacgccgtc gaggcgcgac tgacggggac cgtcgagacc gcgctccaga gcattgtcaa
      841 ggctatgctt gcggtgcgcg ccgcacgcca ggcggcggtc gacacgccgg ccgagtaccg
      901 ccgcatcggc gccgcttgcg gctgagcgac cggccgcata ggttgcccgg agggagggaa
      961 tccgggatgc gcggccatca cgatatggga gggctgccgg cggggccggt cgagcgcagc
     1021 gagcacgacc atgcgctttg ggagaagcgc gtcgacgcgc tcatgctgct gctgtcggcg
     1081 ccgagccggc ggctgctgcg tgtcgacgag ctgcgccgca acattgaagc gctgccaccc
     1141 gatgcctatg accgcatgac gtactacgag cgctggatct cggcgattgc caacacgctc
     1201 ctccagcgcg gcgtcatcac cgccgatgag ctcggccgca aaatggccgc ggtcgccgcg
     1261 cgcgggaagg acgcgccgtg agcgagaccg cgacactggc gccgcgcttc gcccccggcg
     1321 cgcgcgtgtc ggtgcggcgc gccgatccgc cggggcattt gcgcacgccc tggtatatcc
     1381 ggggtcatgt cggcgtgatc gagcgcctgt gcggcgcttt tgccaatccg gaggagcttg
     1441 cctacaaccg ccccggcgtg ccgaagcagc cgctctatcg cgtgcgcttc cgccagcgcg
     1501 aggtttggcc cgactatcgc ggctccgccg aagacaagat cgaagtcgag atctatcagc
     1561 attggctcga gcccgcgtga catgacgacg cattcgcatc cgcatccgca cgagacgctg
     1621 ccggagcacg acgagcgcct cggctactac cagacgatgg agatcgcggt ccgcgagctg
     1681 ctcatcgcca aagcggtctt caccgccgat gacatgcgcc gcgctgtcga ggccatggat
     1741 gcgcgaacgc cggcgcaggg cgcgcgcgtg gtggcccgtg cgtggtgcga tccggctttc
     1801 aaggcgcggc tcatcgagga cggcacggcc gcggtcggcg agctcggcat cgacatggga
     1861 gagacgcgcc tcctcgttgt cgagaatcgg ccgggtgtgc acaacctcgt cgtctgcacg
     1921 ctgtgctcct gctatccccg catgctgctg ggcctgccgc ctgactggta caagagccgc
     1981 gcctatcgca gccgcgccgt gcgcgagccg cgcgccgtcc tggccgagtt cggcaccgtg
     2041 cttcccgaag acgtcgctgt ccgcgtccat gattcgacgg ccgacatgcg ttacctcgtg
     2101 ctgccggcgc agccgcccgg gaccgcgggc tggagcgagg agcgccttgc cgcgctggtc
     2161 ggccgcgacg ccatgatcgg cgtcacgcgc cccgttctgt gaagcctttc acagacgccg
     2221 ggcgccgacg gcagctaggg tcgctttcgt tatcgcgctt tcctcccaaa ctcgagcccc
     2281 ggcctcgcgc cggggctttt ttgctgcgcg caatcgggat gcaggaccgt tctgcggccg
     2341 atgattgctg ctaatatgcg cggcaaatag cgagggcgag gggagggatg gcaatccgcg
     2401 ccgagcagca gaaggacgac gccgtcgaaa gcgtggtggc gctggctgcc aagcgcctcg
     2461 gcggcgatcg cgccggggat gtcgcgcgtt tcttgcgact gttctacagc catgttccgc
     2521 cggccgacat cctcgagcgc gcgccggaag atctctatgg cgcggcggtg tcgctgtggc
     2581 ggttcgccga gacgcggcag cccggccgcg ccaagctgcg cgtcatcaat ccgcgcatcg
     2641 aggagcaggg gtggcgctcg gaccggacgg tcgtcgagat cgtcaatgac gacatgccgt
     2701 tcctcgtcga ttccgtcacg gcggcgctcg ccggcgaagg cttcaccgtc catctcgtga
     2761 tccaccccgt cctgcgcgtc gagcgcgacg ccgagggccg cctcgcggcg ctgctcgaca
     2821 gcggcggcgg caatggcaat ggcaatggcg cgggcggcgg cttgcgcgaa tccgtcatgc
     2881 atgtcgaggt gagcgaggag cgcgatcccg cgcgcctgga tgcgctggcg gcgttcttgc
     2941 agcacgtgct cgacgaggtg cgtgccgcgg tccaggactg gatgcgcatg cgcgagcggc
     3001 tgcgtgcgat cgccgccgag ctcgccgccc agccaccgcc gctcgtcgag gcggagcgcc
     3061 gcgaggggct cgatttcctg gcctggctcg acgacgacaa cttcaccttc ctcggcttcc
     3121 gcgagtaccg tttcggcggc ggccaggaga gcggcctcga catcctgccc gggagcggcc
     3181 tcggcatcct ccgcgacgaa ggccacaccg tcttcgacgg gctccgccac ttcgcgacac
     3241 tgccgcccga tgtgcgccag ttcctgcgcg agccgcggct cctgatgatc accaagtcga
     3301 accgccgctc ctgcgtccac cgcgcggtct atatggacgc gatctgcatc aagagcttcg
     3361 acgcgtcggg caatgccatc ggcgagcggc ttttcgtcgg tctcttcacc tcgctcgcct
     3421 atagccggag cccgcgcgcc attccgctgc tgcggcgcaa ggtcgagacc gcgctcaagc
     3481 gctccggctt cgagtcgcag agccatgacg gcaaggcgtt gctccacatt ctcgatacct
     3541 atccgcgcga cgagctcttc cagatcggcg aggacgagct gttcgagaca gtgctcggga
     3601 tcctccatct tcaggagcgc cagcgcatcg cgctcttctt gcgccgcgat cctttcgagc
     3661 gcttcgtctc ctgtctcgtc tatgtgccgc gcgagcgtta cgacacggcg ttgcgcgagc
     3721 gtttcgtcca catcctcgag gacgcccttg ccggcaaggt cgacagcttc aacgcccagc
     3781 tcgatgaatc cgtcctcgcc cgcattcatt tcatcgtgcg gacgattccc ggccggctcg
     3841 cccagatcga tgtgggcgct ctcgagcgcc gtcttgccga ggcgggacgg agctgggccg
     3901 accgcctcgc cgaggcgctt gccgaggcga aaggcgaagg gccgagccgc gcccttttgc
     3961 gacgctatgc cgaggccttt cccaccggct accgcgagcg cttccccgcg gcggccgcgg
     4021 tctacgacat cgagcgcctc gaggagctgg cgcaaggcgc gcccatcgcc gtgacgctct
     4081 accgtccgct cgaggccgaa tcgcgcgagc tgcgcttcaa gatctatcgc cagggcgcgc
     4141 cggtgccgct gtcggacgtg ttgccggtcc tggagcatct ggggctcagg gtcatcgccg
     4201 aaatccccta tgccgtcgcc cccatgggcg agcccgcggc gtcgcttcag gatttcagct
     4261 tgacggtgcc gtcgggcgag gtcgacgtgg cgcgcgaccg cgcccgcttc gaggaggcct
     4321 tcctccggct ctgggcgggc gtcatggaga gcgacggcct caaccgcctg gtgctcgccg
     4381 ccggcctcga ctggcggcag gtggtgatgc tgcggctcta cgtgaaagtg ctgcgccagg
     4441 ccggcagcgc gtatagccag gcctatatgg aggacgcgct cgcgggccat gcggcgatcg
     4501 cgcgccagct cgcccagctg ttcgagcggc gcttcgatcc caaacgcgag cgcggccgcg
     4561 acgccgagac ggcgaagctg gtgcaggcga gcgagcgcgc gctcgaggat gtcgcgagtc
     4621 tcgacgagga ccgcatcctc cgcagcatgc tgctgctcgt gacgaagtcg ctgcgcacca
     4681 actacttcca gcgcgacgcg ggcggcgagg cgaagcccta tctctccgtg aagctggcga
     4741 gccgcgagat cgatctgctg ccgctgccgc ggccgctcta tgagatctac gtgctgagcc
     4801 cccgggtcga gggcgtgcat ttgcgcggcg gcaaggtggc gcgcggcggc atccgctggt
     4861 ccgatcgcaa ggaggatttc cgcaccgaga tcctgggcct gatgaaggcg cagatggtca
     4921 agaacgcggt gatcgtgccg acgggctcga aaggcggttt cgtggtgaag cgaccgcccg
     4981 cgacgcgcga cgcgctccag gccgaaggca tcgagtgcta caagaccttg ctgcgcggcc
     5041 tcctcgatct cacggacaac atcgtcggag cgaagatcgt gccgccgcgc gacgtcgtcc
     5101 gtcatgacgg cgatgatccc tatctcgtcg tcgccgccga caagggaacc gc
//
LOCUS       DASYNS010000252         8079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3959766, whole genome shotgun sequence.
ACCESSION   DASYNS010000252 DASYNS010000000
VERSION     DASYNS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3959766"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..301
                     /locus_tag="VGP50_07935"
     CDS             83..301
                     /locus_tag="VGP50_07935"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEV8027346.1"
                     /translation="MLNSIGNLLHRTADAMARRRELRRAHAELSALDDRSLADIGISR
                     SEIAYALTHSTRAHTPATPVAAELRHAA"
     gene            431..1345
                     /locus_tag="VGP50_07940"
     CDS             431..1345
                     /locus_tag="VGP50_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV8027347.1"
                     /translation="MTEEAHRAGAGFALAAFIGVLLNLGFVVIETIYGVVGHSVALLA
                     DAGHNLGDVLGLAAAGIATLLAQRRPSHRYTYGLRSSSILAALFNAIVLLIAVGAIAV
                     EAIGRLLEPEPVATGTVMAVAAIGILVNGATALVLRHGHGHGHAHDLNIRAAFLHMIG
                     DTAVSLGVVLASAAILVTGWLWLDPAASLVIALVIVAGTWQLLRQALDLALHAVPSGI
                     EAAAVRDHLARSAGVCAVHDLHIWAMSTTEAALTCHLVMPSGHPGDASLAHLAEELHD
                     RFGIAHATIQVETGDPDHPCKLEADHVV"
     gene            1373..3058
                     /locus_tag="VGP50_07945"
     CDS             1373..3058
                     /locus_tag="VGP50_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEV8027348.1"
                     /translation="MQASDGGQLHAVLILLLAAIVAVPLAQRLRAPPMLGYLAAGLAV
                     GPFGLGLVENTAEAEQLSPFGVVFLLFTIGLDLPLARLQAMWRYIFGLGMVQMIVTAA
                     LVAAIAYALGADIRAALVIGSALAFSSTAAVVQLLAERGELASRAGRIAIAVLLLQDL
                     SVVPLLALLPLLRPGGGGIVAALAIALGKGAAALILILLLGRLVLRPLFRLVARTRIA
                     ETFAATVLLVVLGTGWATAQAGMSMALGAFLAGLLLAETEYRHQVEVDIQPFRGLFLG
                     LFFITVGMVIDIPVLVGHWVAVLVIAGALLALKSLLLAVLARLFGLVWPLAVRTALLL
                     SQGGEFAFVVFAMAMALSPPILGHDTGEVLIAAVTLTMATTPAMAALGRHAAARLQAT
                     PAPESQRMSEETEDLSGHVVIAGYGRVGQIVGQLLAAQQIPYVAIEQDVALVEAHRRH
                     DRPVYFGDASRPELLEAAGLRRASGAVITLNQPAAAERLVAALRRAHPTLHIIARSHD
                     VEQQRRLQEAGADAVILEALEPGLQMGAAVLRGTGAPSDAVDHALEAFRRSNQ"
     gene            complement(3104..3709)
                     /locus_tag="VGP50_07950"
     CDS             complement(3104..3709)
                     /locus_tag="VGP50_07950"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEV8027349.1"
                     /translation="MAMNDPDEDLNSRAKRRAVSEPSADSGKSFRFALIMVGACVVVS
                     LVVGRLLPELAPAPAKVSLTAAAVTPPARGAPKTVANKISYPADPSGHFFVDAMVNGV
                     EIRFLVDTGATFVALSPDDAAAAGIAPQNLSFDEATNTANGVSHVARTSLRSIRLGQL
                     EITDVAAVVVEQSMPVSLLGMSFLNRVDGYSIRDGVLTIEW"
     gene            complement(3773..3985)
                     /locus_tag="VGP50_07955"
     CDS             complement(3773..3985)
                     /locus_tag="VGP50_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027350.1"
                     /translation="MSVTTVVDFQPSREVDLSSIRRISGRVRIRRAIGMALMLLAVTA
                     GIQGGMINLQRQFEAARHASVAPLAR"
     gene            4254..5078
                     /locus_tag="VGP50_07960"
     CDS             4254..5078
                     /locus_tag="VGP50_07960"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEV8027351.1"
                     /translation="MSGPLSGRRIVVPETRELDLFARMLEDRGAEVLRCPMVAIVDAP
                     DPAPIDAWLRRVIAGACDDLILFTGEGLRRLLGSARRSGLDQVFVAALTRLRKITRGP
                     KPVRALREIGLDADLAAQPPTTEGVIATLAALDLRGRRVGVQLYPDSTHDALLGFLQA
                     AGAAADPVLPYAYVAAADDRQVIGIIDRMADGEVDAIAFTSTPQVRRLYKVAVAAGYE
                     ARLGAALLRTKVASVGPVVGDELRRIGARVDIATDGSYFMKPLVNEIVARLGQATT"
     gene            5198..5797
                     /locus_tag="VGP50_07965"
     CDS             5198..5797
                     /locus_tag="VGP50_07965"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV8027352.1"
                     /translation="MAWLEPATLRGRHATLEPLAQHHHDGLVEAVKDGELWKLWYTSI
                     PAPEKMAAEIDRRLALQASGTMLPFTVIDQEARRPIGMTTYMNVDRANRRVEIGSTWY
                     ARSAQRTPINTECKLLLLTHAFEALDCIAVEFRTHFFNHRSRRGIERLGAKLDGVLRN
                     HQIVANGTLRDTCVYSIIVGEWPAVKSHLAWQMTKPGDD"
     gene            5824..7992
                     /locus_tag="VGP50_07970"
     CDS             5824..7992
                     /locus_tag="VGP50_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027353.1"
                     /translation="MIGNRPIAASLIAIGLAMLLAGAPGARGAEPLSNAAIAIGDNDL
                     GGTVRGANGPEAGVWVIAETTDLPTKFAKIVVTDERGRYVLPDLPKASYSVWVRGYGL
                     VDSPKVWSVPGKILDLTAVPAPNAAAAAEYYPAIYWYSMLKVPDKSAFPGTGPNGNGM
                     PENLKSQAQWLDVVKTNGCYTCHQLGDKATRTIPSELGHFESSIDAWERRIQSGQAMT
                     SMVNNIGRLDTKRALSLFADWTDRIAGGELPATQPPRPQGVERNIVITLWDWAGPKDY
                     LHDEISTDKRNPSVNANGPIFGTTEESRDLFPILDPGANKATQVKMPVRDPDTPSSKT
                     LPMAPSPYWGDAPIWDSQASMHNPMLDAKGRVWFTSRVRGPETPAFCRKGSDHPSAKF
                     FPLDQSNRQLAMYDPKTGKIALIDTCFPTHHLVFASDADDTLWMSAGGPASGVVGWFN
                     TKLFDETGDAQKAQGWTALVLDTNGNGKRDDYVGPGDAVDPTKDKRVNAALYGIAVSP
                     VDGTVWGSVLGFPGYVIHVIPGSNPPSTALAEIFEPPFPAGYGPRGMDIDRNGVVWTP
                     LSSGHLASFDRRKCTGPLNGPNATGRQCPEGWTLYPFPGPQLRNVTESGSAEASYYTW
                     VDQFNTSGLGRNVPIATGNANDALLALVDGKFVVLRVPYPMGFYAKGMDGRIDDAGAG
                     WKGKALWATYGTRTPFHAEGGKGTTSKVVKFQLRPDPLAR"
BASE COUNT         1288 a         2775 c         2673 g         1343 t
ORIGIN      
        1 agagaattaa tcttcgatca accatgacca cattattgtg catgagcgag attatttcgc
       61 agttgcgttc ggagttacta agatgctgaa ttcaattggt aatctgctgc accgcacggc
      121 cgatgccatg gccaggcggc gcgagttgcg gcgcgcccat gccgagctct cggcgctcga
      181 tgaccgctcg ctcgccgata tcggcatcag ccgctccgag atcgcctatg ccctgacgca
      241 cagcacgcgg gcgcacaccc ccgcgacgcc ggttgccgcc gagcttcgcc acgccgcctg
      301 acgcgggcgt tgcccgcaac gacttgcacg gcgaagccgc cgcggcgatg ttgattgccg
      361 tagaggcagt cacagggact gatcccgcgg actcgcgggt cggttggatg caatgccgca
      421 tggcgaggca atgaccgagg aagcacaccg cgcgggcgcc ggattcgcgc tggcggcctt
      481 catcggcgtg ctcctcaatc ttggcttcgt cgtcatcgag acgatctatg gcgtcgtcgg
      541 acattcggtg gcattgctcg ccgacgccgg tcacaatctt ggcgacgtgc tgggcctggc
      601 ggccgcggga atagcgactc tgctggcgca gcggcggccg agccatcgct acacttatgg
      661 cctgcgctcg agctcgattc tcgccgcgct cttcaatgcc atcgtcctgc tgatcgcggt
      721 cggcgccatc gccgtcgaag cgatcgggcg gctcctcgag ccggagccgg tggcgaccgg
      781 caccgtcatg gccgtcgccg ccatcggcat tctcgtcaac ggcgccacgg cgctggtcct
      841 gcgccatggc catggccatg gtcatgcgca tgacctcaac atccgcgccg cattcctgca
      901 tatgatcggc gataccgcgg tgtctctcgg cgtggttctg gccagcgcgg cgattctcgt
      961 caccggctgg ctttggctcg atccggcggc gagcctcgtc attgcgctgg tcatcgtcgc
     1021 gggcacgtgg cagctcttgc gtcaagcgct cgatctggcg ctgcatgccg tgccgtccgg
     1081 catcgaggcg gccgcggtac gcgaccatct cgcgaggagc gccggggttt gcgccgtaca
     1141 cgaccttcat atttgggcga tgagcaccac cgaagcggcg ctgacctgcc atctcgtgat
     1201 gccctcggga catcccggcg acgcatcgct ggcgcatctc gctgaggagc tgcatgaccg
     1261 cttcggcatc gcgcatgcga cgatccaggt cgagaccggc gaccccgatc atccttgcaa
     1321 gctcgaagca gatcacgtcg tataggccgg gctcccaaga ggtggcggcc gcgtgcaagc
     1381 cagcgatggt ggacagctcc acgccgttct gatcctgctg cttgcggcga tcgtcgcggt
     1441 gccgctggcg caacggctgc gcgcgccgcc catgctgggc tacctcgcag cgggtctggc
     1501 ggtcggtccg ttcggactgg gcctcgtcga gaacacggcc gaggccgagc agctctcgcc
     1561 tttcggcgtg gtcttcctgc tgttcaccat cggcctcgac ctgccgctgg cgcggctgca
     1621 ggcgatgtgg cgctacatct tcggcctcgg catggtgcag atgatcgtga ccgcggcgct
     1681 cgtcgccgcg atcgcctatg cgctcggcgc cgacatccgc gccgccctcg tcatcggcag
     1741 cgccctcgcc ttctcgtcca cggccgcagt ggtacaactg ctcgccgagc gcggcgagct
     1801 ggcgagccgc gccggccgca tcgcgatcgc cgtcctgctg ctccaggatc tctcggtcgt
     1861 gccgctcctc gcgctgctgc cgctgcttcg tccgggcggc ggcggcatcg tcgcggcgct
     1921 cgcgatcgcg ctcggcaagg gcgcggcggc gctgatcctg attctgctgc tgggccggct
     1981 tgtgttgcgt ccgctcttcc ggctcgtggc acgcacgcgc atcgccgaga ctttcgcggc
     2041 gaccgtcctc ctggtggtgc tcggcaccgg ctgggcgacg gcgcaggcgg gaatgtcgat
     2101 ggcgctgggc gccttcctcg ccggcctgct gctcgccgag accgagtacc gccaccaggt
     2161 cgaagtcgac attcagccct tccgcggtct gttcctcggc ctcttcttca tcacggtcgg
     2221 catggtcatc gacatccccg tgctcgtcgg ccactgggtc gccgtgctgg tgattgccgg
     2281 ggcgttgctc gcgctcaaat cgctgctgct cgccgttctc gcgcggctct tcgggctcgt
     2341 ctggccgctg gcggtacgga ccgccttgct gctgtcgcaa ggcggcgagt tcgccttcgt
     2401 cgttttcgcg atggcgatgg cgctgtcgcc gcccatcctc ggccatgaca caggcgaggt
     2461 cctgatcgcc gcagtgaccc tgaccatggc gacgacaccc gccatggccg cgctcgggcg
     2521 gcacgcggcg gcgcggctgc aggcaacccc ggcgcccgag tcgcagcgga tgagcgagga
     2581 aacggaggat ctctcgggcc atgtggtgat cgccggttat gggcgcgtcg gccagatcgt
     2641 cggacagctg ctcgcagcgc agcagatccc ctatgtcgcg atcgagcagg acgtggcgct
     2701 cgtcgaggcg catcgccgcc atgaccgtcc tgtgtatttc ggcgatgcga gccggccgga
     2761 gctgctcgag gcggcagggc tccggcgcgc gagcggcgcc gtcatcaccc tcaaccagcc
     2821 cgccgccgcc gagcggctgg tcgcggcgct caggcgcgcc catccgacgc tgcacatcat
     2881 cgcgcgcagt cacgatgtcg agcaacagcg ccggctccaa gaggccggtg ccgatgcggt
     2941 gatcctcgag gcgctggaac ccgggctgca gatgggcgcc gccgtgctgc gcggcaccgg
     3001 cgcgccctcc gatgccgtcg accacgccct cgaagcattc cggcgcagca atcagtagac
     3061 aatcggcggt cgacaggagc ggacaaggtc ctgttgactg tcactaccac tcgatcgtca
     3121 gaacgccatc gcggatgctg taaccgtcga cccggttgag gaagctcatg ccgagcagcg
     3181 ataccggcat cgactgttcc accaccaccg cggcgacatc ggtgatctcg agctggccaa
     3241 ggcggatgct gcgcaggctg gtgcgcgcca catgcgacac gccgttggcc gtgttggtcg
     3301 cctcgtcgaa actcaaattc tgcggtgcga tcccggcggc agcggcgtcg tccggcgaga
     3361 gcgccacgaa ggtcgcgccc gtatcgacga gaaagcgtat ttccacgccg ttgaccatgg
     3421 cgtcgacgaa gaaatggccg ctgggatccg cgggatagct gatcttgttg gcgacggtct
     3481 ttggcgcgcc gcgcgcgggc ggcgtgacgg ccgcagcggt gagcgacact ttggccggcg
     3541 ccggcgcgag ctccggcagc agccgtccga cgaccaggct tacaacgacg caggcgccga
     3601 ccatgatgag cgcgaagcgg aagctcttgc ctgagtccgc cgacggctcg ctgaccgcgc
     3661 gacgcttggc gcgggagttc agatcctcgt cggggtcgtt cattgccatc gcgggacgct
     3721 ggcccgtgcc ggcgcgtcgg aagaggacga gatgctcgga atgcgctggg tcctacctcg
     3781 cgaggggcgc gacggaggca tgccgggcag cctcgaactg ccgttgcaga ttgatcatgc
     3841 cgccctgtat gccggccgtc accgcgagca gcatcaacgc catcccgatg gcgcggcgga
     3901 ttcgcacacg cccggatata cggcggatgc tcgagaggtc gacctctcgc gagggctgga
     3961 aatcgacgac ggtcgtgacc gacatggaca tctccttgcg cttcgtcctc cacattgcgg
     4021 caccttcgcg atccgcaata gtcggcgcgt cgtctcgttt gtttggctcg gccaatccgg
     4081 tggccgcttg gccggatggc ctatgccgtc gcgatgccaa gaaccattcg cgtgaagcaa
     4141 atcgccattt tactccggcg gatagcgggg tcccccctcg ggagcggaaa cgcgccaaat
     4201 agtcctcgtt gcgctacact cgcggtcatg agcgtcgccg agcgtgatca ccaatgtccg
     4261 ggcctctgag cggacgccgg atcgtcgttc ccgagacgcg cgagctggac ctgttcgcgc
     4321 gcatgcttga agaccgcggc gccgaggttc tgcgctgccc gatggtcgcc attgtcgatg
     4381 ctcccgatcc cgcgccaatt gacgcctggc tgcgccgcgt gatcgccggc gcctgcgacg
     4441 acctcatcct gttcacggga gagggcttgc ggcgcctcct cggctctgcg cgacggtcag
     4501 gcctggacca ggtcttcgtc gccgcgctga cgcggttgcg caagatcacg cgcggcccta
     4561 agccggtgcg cgcgttgcgc gagatcggcc tcgatgccga ccttgccgcg cagccgccga
     4621 cgaccgaggg cgtcatcgcc acgctcgccg ccctcgatct caggggtcgc cgggtcggcg
     4681 tgcagcttta tccggacagc acgcatgacg cgctgctcgg ctttcttcaa gccgccggcg
     4741 cggcagccga tcccgtcctg ccctatgctt acgtcgcggc cgccgacgac cggcaagtca
     4801 tcggcatcat cgaccggatg gccgacgggg aggtcgacgc gattgccttc acgagcacgc
     4861 cgcaagtgcg gcgcctctac aaggtcgccg tcgctgcggg ttatgaggcc cggttgggcg
     4921 cggcgcttct ccgcaccaag gtggcgagcg tcggaccggt ggtcggggac gagttgcgcc
     4981 ggatcggtgc gcgcgtcgac atcgccaccg acggcagcta cttcatgaag ccgctggtca
     5041 acgagatcgt cgcccggctc ggccaggcca cgacctagcg cgccgcgacc ggcttctctc
     5101 catgatcgct tgattgccgt gcggtatggg gggtggactc tccgccgccg tgggtcgaga
     5161 ctcgcccacg ggcgaggaga tggcgggagg acgagcaatg gcatggctcg agccggcgac
     5221 gttgcgcggt cgacacgcga cgctggagcc gctggcgcag caccatcatg acggtcttgt
     5281 cgaggcggtg aaggacggcg agctctggaa gctctggtat acgagcatcc cggcgccgga
     5341 aaagatggcc gcggagatcg atcgtcgcct cgcgcttcag gcgagcggca ccatgctgcc
     5401 cttcaccgtc atcgaccagg aggcgcgccg gccgatcggc atgacgacct acatgaacgt
     5461 cgatcgcgcc aatcgccgcg tcgagatcgg ctcgacatgg tatgcccgga gcgcgcagcg
     5521 gacgcccatc aacaccgaat gcaagctgct cctgctgacg cacgccttcg aggcgctcga
     5581 ctgcatcgcc gtcgagttcc gcacgcattt cttcaatcac cggagccggc gcggcatcga
     5641 gcgtctcggc gcgaagctcg acggcgtcct ccgaaaccat cagatcgtcg cgaacgggac
     5701 actgcgcgat acttgcgttt acagcatcat cgtcggtgaa tggccggcgg tgaagtcgca
     5761 tctcgcctgg cagatgacca agccgggcga cgactgaaac aacgaaacgg gaggagcgcc
     5821 agcatgatcg gcaaccgtcc aattgctgcg agcctgatcg cgatcggtct tgccatgctc
     5881 ctggcgggcg cgcccggcgc ccgcggcgcg gagccgctta gcaatgccgc catcgcgatc
     5941 ggcgacaacg acctcggcgg caccgtcaga ggcgccaacg ggccggaggc cggcgtctgg
     6001 gtcatcgccg agacgacgga cctgccgacg aaattcgcca agatcgtcgt caccgacgag
     6061 cgcggccgct atgtgcttcc cgatcttccg aaggccagct acagcgtctg ggtgcgcggc
     6121 tatggtctcg tcgattcgcc caaagtctgg agcgtccccg gcaagatcct cgatctgacg
     6181 gcagtgcccg cgccgaatgc agcggccgcc gcggaatatt atccggccat ttactggtat
     6241 tcgatgctga aggtgcccga caaaagcgcg ttccccggca ccggacccaa cggcaacggc
     6301 atgccggaaa atttgaagag ccaggcgcaa tggctcgacg tcgtcaagac caatggctgc
     6361 tacacctgcc accagctcgg cgacaaggcg acgcgcacca tcccgagcga gctcgggcat
     6421 ttcgagagct cgatcgacgc gtgggagcgg cgcatccagt cgggccaggc gatgaccagc
     6481 atggtcaaca acatcggccg gctcgacacc aagcgcgcgc tgagtctgtt cgccgactgg
     6541 acggaccgca tcgccggcgg cgaattgccg gcgacacagc cgccgcgccc gcagggcgtc
     6601 gagcgcaaca tcgtcatcac gctctgggac tgggcggggc ccaaagacta tctccacgac
     6661 gagatctcga cggacaagcg caacccctcc gtcaatgcca atggcccgat cttcggcacg
     6721 accgaggaga gccgcgatct cttcccaatc ctcgatcctg gcgccaacaa ggcgacgcag
     6781 gtgaagatgc ccgtgcgcga cccggacacg ccgtcgtcca agaccctgcc gatggcgccc
     6841 tcaccctatt ggggcgacgc gcccatctgg gacagccagg cgagcatgca caatccgatg
     6901 ctcgacgcga aaggacgggt ctggttcact tcgcgggttc gcgggccgga gactccggcg
     6961 ttttgccgaa agggatcgga ccatccgtca gcgaagttct tcccgctcga ccagtcgaac
     7021 cgccagctcg cgatgtacga cccgaagacc ggcaagatcg cgctgatcga cacatgcttt
     7081 cccacgcatc atctggtttt cgcgtccgac gccgacgaca cgctctggat gagcgccggc
     7141 gggccggcga gcggcgtcgt cggctggttc aacacgaagc tcttcgacga gaccggcgac
     7201 gcgcagaagg cacagggctg gacggcgctg gtgctcgata ccaacggcaa cggcaagcgc
     7261 gacgactatg tcgggccggg cgacgcggtc gatccgacca aggacaagcg cgtcaacgcc
     7321 gccttgtacg gcatcgccgt cagccccgtc gacggcacgg tgtggggctc ggtgctcggc
     7381 ttcccgggct atgtgatcca tgtcattccc ggatctaacc cgccatcgac cgctctcgcc
     7441 gagatcttcg agccgccctt ccccgccggc tacggcccgc gcggcatgga catcgatcgc
     7501 aacggcgtcg tctggacgcc gctgtcgagc ggtcatctcg ccagcttcga ccggcgcaaa
     7561 tgcacgggac cgctcaacgg gccaaacgcc accggcaggc aatgtccgga gggctggacg
     7621 ctctatccct tccccggacc gcaactccgg aacgtgacgg agtcgggcag cgcggaggcc
     7681 agctactaca cctgggtcga ccagttcaac acctccggtc tcggccgcaa cgtgccgatc
     7741 gccaccggca acgctaatga cgcgctgctg gcgctggtcg acggcaagtt cgtggtgctg
     7801 cgcgtcccct atcccatggg cttctacgcc aagggcatgg atgggcgcat cgacgatgcc
     7861 ggcgcgggct ggaagggcaa ggcgctctgg gcgacctatg gcacgcggac acccttccac
     7921 gcggaaggcg gcaaaggcac gaccagcaag gtggtgaagt tccagctccg gcccgatccg
     7981 ctggcgcggt gagcccgacg cgaccgcctc aattcgccgc gggcgccagc gacagaaagc
     8041 gcgacagcag cgccgaccag cgctcgtggt cgaagcccg
//
LOCUS       DASYNS010000253         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3023784, whole genome shotgun sequence.
ACCESSION   DASYNS010000253 DASYNS010000000
VERSION     DASYNS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3023784"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1617)
                     /locus_tag="VGP50_07975"
     CDS             complement(118..1617)
                     /locus_tag="VGP50_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV8027354.1"
                     /translation="MLQNRLEPVLDPRALARMLSNKLLIDGALAPAATGRTFPVISPA
                     TQEHIADAAEGLAADVDIAVKAAAKAQKAWAKLASRERGRLVYDCGRLLNEHVEEIAR
                     LITLETGKAIRTESRVEAGVFADVLIYYGGLGSELKGESVPFKPGMLAITQREPIGVV
                     GAIIPWNVPLLLMALKIAPALVAGNGVVVKSAEEAPLAVLRACQIMNQVLPPGILNIL
                     SGDGPGCGAPLVAHKGVGKVTFTGSVETGKLVYRMASEKLIPVTLELGGKSPMIVMGD
                     ADLDKAIDGAVIGMRFTRQGQSCTAASRIFVHDSIHDAFVQKLKAKVDAMRMGDPLDE
                     ATDIGSIISKQQFDKVKNYIRIGEETPGAKALRCSAMPADQKLAQGYFVQPVIFTGIA
                     NDNRICREEIFGPVTCVIKFRDYEDAIRQANDSDYGLAATIWTRDLKTALDATQRLEA
                     GLVQVNQNLVVQANMSYGGVKQSGLGKEASLEAMLEHFTIKKTIILNLG"
     gene            complement(1773..2930)
                     /locus_tag="VGP50_07980"
     CDS             complement(1773..2930)
                     /locus_tag="VGP50_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEV8027355.1"
                     /translation="MAVRGPLSGVTVVDLSRILAGPYCTLLMAELGARVIKVEPPGKG
                     DDAREYGPFRNGKSTYFSSVNRGKESIALDLKSPADKRIFGQLLERADVLVENFRPGT
                     LEKLGYGWETLHRAFPRLIYAAASGFGHTGPHSRHPAYDMVVQGVGGIMSITGHPGQP
                     PVRIGTSIGDIGAGLYTAVAVNAALLHREKTGEATKVDVAMFDCQLALLENAIMRYTV
                     TGEIPGPLGARHPSIAPFEAFATADGAMIIAAGNDGLFQKLCDALGRPELARNADYGT
                     NDLRCQNEPALKREIEAVLRLKPTAAWIAVLEAAGIPCGPINNVAEALRHPQVVPRNM
                     LVTVEDPVSGTLELAGNPLKFSAFDDPATRPPAPELDADRARLLRELGIEG"
     gene            complement(3159..3335)
                     /locus_tag="VGP50_07985"
     CDS             complement(3159..3335)
                     /locus_tag="VGP50_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027356.1"
                     /translation="MISEIPLTCPHCGKATVKTVDWVQQNTFFTCVCCGAAVMIDKDM
                     AAQLLAQLELAQRR"
     gene            complement(3418..>3853)
                     /locus_tag="VGP50_07990"
     CDS             complement(3418..>3853)
                     /locus_tag="VGP50_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8027357.1"
                     /translation="PRAEDQADWRRLYDGYAAFYKSPMDDAIAGRVWGWLLDPQHPLE
                     ALVGRTAAGRVVGLAHFRAMPRPLSGSMAGFLDDLFVDPEWRGRRVADRLIAAVAVLG
                     RQRQWTRLRWITADDNYRGRAVYDRVAKRTMWITYEMDLATS"
BASE COUNT          623 a         1267 c         1319 g          644 t
ORIGIN      
        1 aaccctcccc ctcaaggggg gaggggagaa atgcgtaggg cgagagcgat cttccccctc
       61 cccccttgag ggggagggag gggtgggggg tgatcgcacg gaccacaacc atcatgctca
      121 ccccagattc aaaatgatcg tcttcttgat cgtgaaatgc tcgagcatcg cttcgagcga
      181 cgcctctttg ccgagccccg attgcttcac gccgccatag gacatgttgg cttggaccac
      241 gagattctgg ttcacctgca cgaggcccgc ctcgaggcgc tgggtcgcat cgagcgccgt
      301 cttgaggtcg cgcgtccaga tggtggcggc gagcccgtag tcgctgtcat tggcctggcg
      361 gatcgcgtcc tcgtagtcgc ggaacttgat gacgcaggtg acggggccga agatctcctc
      421 gcggcagatc cggttgtcgt tggcgatgcc ggtgaagatc acgggctgta cgaaataccc
      481 ctgggcgagc ttctggtccg ccggcatggc cgagcagcgc agcgccttgg cgcccggcgt
      541 ctcttcgccg atccggatat agttcttcac cttgtcgaat tgctgcttcg agatgatcga
      601 gccgatatcg gtcgcctcgt cgagcggatc gcccatccgc atggcgtcga ccttcgcctt
      661 cagcttctgg acgaaggcgt cgtggatcga gtcgtggacg aagatgcggc tcgctgcggt
      721 gcagctctgc ccctggcgcg tgaagcgcat gccgatgacg gcgccgtcga tggccttgtc
      781 gagatccgcg tcgcccatga cgatcatcgg gctcttgccg ccgagctcga gcgtgacggg
      841 gatgagcttc tccgatgcca tcctgtagac gagcttgccg gtctcgacgg agcccgtgaa
      901 ggtgaccttg ccgacgccct tatgcgcgac cagcggcgcg ccgcagccgg gcccatcgcc
      961 cgacaggatg ttgaggatgc cgggcggcag cacctggttc atgatctggc aggcgcgcag
     1021 caccgcgagc ggcgcttcct cggccgactt cacgacgacg ccattgccgg cgaccagcgc
     1081 gggcgcgatc ttgagcgcca tcagcaagag cggcacgttc cagggaatga tggcgccgac
     1141 cacgccgatc ggctcgcgct gggtgatggc gagcatgccc ggcttgaagg gcacgctctc
     1201 gcccttgagc tcggagccga ggccgccata gtagatcagc acatcggcga agacgcccgc
     1261 ctcgacgcgg ctctcggtgc ggatcgcctt gccggtctcg agggtgatga gccgcgcgat
     1321 ctcctcgaca tgctcgttga gcagccggcc gcagtcataa acgagccggc cgcgctcgcg
     1381 cgacgcgagc ttcgcccagg ccttctgcgc tttggcggcg gctttcacgg cgatgtcgac
     1441 atcggccgcg agaccctcgg cggcatcggc gatgtgctcc tgcgtggccg ggctgatcac
     1501 cgggaaggtc cgccccgtcg ccgccggcgc caacgcgccg tcgatcaaaa gcttgttcga
     1561 cagcatgcgc gccagcgcac gcggatcgag gacgggctca agccggttct gcagcatcga
     1621 cgtctcccag gatactcgcg gtttggcgag aggttataca catccgcggc catgtcgcca
     1681 ccctccgggt ccgcggatgc gtcgatcacg acatgcgttc ctttgaggcc gaacacgacg
     1741 tgccggcggc gtgcgcggcg ccgcgccgcg tctcagccct cgatcccgag ctcgcgcaga
     1801 agccgcgcgc gatcggcgtc gagctcgggg gcgggtgggc gtgtcgccgg gtcgtcgaag
     1861 gccgagaatt tgagcgggtt gccggcgagc tcgagcgtgc cggagacggg atcctcgacc
     1921 gtcaccagca tgttccgcgg gacgacttgc gggtggcgca gggcctcggc gacgttgttg
     1981 atgggaccgc aagggatgcc ggctgcctcg aggaccgcga tccaagccgc cgtcggcttc
     2041 aggcgcagca ccgcttcgat ctcgcgcttg agcgccggct cgttctggca gcgcagatcg
     2101 ttcgttccgt agtcggcgtt acgggccagc tcgggacgcc cgagcgcgtc gcagagcttc
     2161 tggaacaacc cgtcattgcc ggcggcgatg atcatggcgc cgtcggcagt ggcgaaggcc
     2221 tcgaagggcg cgatcgaagg atgccgcgca cccaaaggcc cggggatctc gcccgtaacg
     2281 gtgtagcgca tgatggcatt ctcgaggagc gccaactggc agtcgaacat ggcgacgtcg
     2341 accttggtgg cttcgcccgt cttctcgcga tggagcagcg ccgcattgac ggcgacggct
     2401 gtgtagaggc cggcgccgat atcgccgatc gacgtgccga tgcgcacggg cggctggccc
     2461 ggatggccgg tgatgctcat gatgccgccc acgccctgca ccaccatgtc gtaagcggga
     2521 tggcgcgaat gcggtccggt atggccgaag ccggacgcgg ccgcgtagat caggcgcgga
     2581 aaggcgcgat gcagcgtctc ccagccatag ccgagcttct cgagcgtgcc gggccggaaa
     2641 ttctccacca gcacatcggc gcgttccagc agctgcccga agatgcgctt gtcggcgggc
     2701 gacttgaggt cgagcgcgat cgattccttg ccgcggttga ccgaggagaa ataggtcgat
     2761 ttgccattgc ggaagggccc gtattcgcgc gcgtcgtcgc ccttgccggg cggctcgacc
     2821 ttgatgacgc gggcgcccag ctcggccatg agcaaggtgc aataaggccc tgccagaatg
     2881 cgcgacaggt cgacgacggt tactcccgac aacggcccgc gcaccgccat ggctcacctt
     2941 gaagattcgc tacggtcgcg ggcgtcttag cgcagggccg ccggcgcgac aatccaagcc
     3001 gccgggcgca gcggaacccg tccgcgccgg gcgtgttcaa tgccgtgctg acgctcgcgg
     3061 cggacatgcc ccaggttcag acgcatgatc gaggttcgaa ggacgaagaa tgaccagccg
     3121 ggcagcgggt ccggccctcg aagcgggtcc ttcgccggtc aacgccgttg cgccagctcg
     3181 agctgggcga gcaactgcgc ggccatgtcc ttgtcgatca tcaccgcggc cccgcagcag
     3241 acgcaggtga aaaacgtatt ctgctgaacc cagtccaccg tcttgaccgt cgccttgccg
     3301 caatgcgggc aagtgagcgg tatctcgctg atcactggcg gtgccttccc cgaccgtccc
     3361 tcatcgtgaa cgcgcacgcg ttccgaccag cgatcaacag ccgccgcgcg caaaggttca
     3421 cgaagtcgcg aggtccattt cgtaggtgat ccacatcgtc cgtttggcga cgcggtcata
     3481 gacggcacga ccgcgataat tgtcgtcggc ggtgatccag cgaagtcgcg tccattgccg
     3541 ctggcgcccg agcacggcga cggccgcgat caatcggtcg gcgacgcgcc ggccgcgcca
     3601 ctccggatcg acgaagaggt cgtcgaggaa gcccgccatg ctgccgctga gcggccgcgg
     3661 catggcgcga aaatgcgcaa gcccgacgac gcggccggcg gcggtccgtc ccacgagcgc
     3721 ttcgagcggg tgctgcgggt cgaggagcca gccccacacc cgtccggcga tcgcgtcatc
     3781 catcggtgat ttgtagaagg cggcgtagcc gtcgtagagg cggcgccaat cggcctggtc
     3841 ctcggcgcgc ggc
//
LOCUS       DASYNS010000254        10101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4584350, whole genome shotgun sequence.
ACCESSION   DASYNS010000254 DASYNS010000000
VERSION     DASYNS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10101
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4584350"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1804
                     /gene="terL"
                     /locus_tag="VGP50_07995"
     CDS             206..1804
                     /gene="terL"
                     /locus_tag="VGP50_07995"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEV8027358.1"
                     /translation="MRGGSSSATEVPERGQIVKALSQADAEESLLDFVRLTWHVIEPS
                     RDFVEGWVIEAICDHLEAVSNGEISRLLINVPPGFTKSFLTDVFWPAWEWGPRDEPTL
                     RYLCFSYSDALTKRDNGRFKNLITSEVYRTLWGDRFGPGEQFGRRKIENDRTGWKFAS
                     SVGGVGTGERGDRVIIDDPNNVKKSESRAVLESTNLWLTEVVPDRLNDLATSAIILIQ
                     QRTNERDCSGTLLDPKLGAEYVHLSIPNEYDPRRHYVNALGWSDPRSSEGALAWPERL
                     PAAETEKLRRIKGSYAYSGQYQQSPAPRGGGIIKRLHWQLWPRGGESFDANGKPAKPL
                     EYPPFDYIVASLDTALGAKEENDWSALTVWGSWRDAYDVPKLMLMEAWQARLEFYPLV
                     QKIIKSCRKRQVDRLLVEAKANGISVAQEIVRLLGEEEFGVTLIDPKKAGGDKVARAY
                     AVQHLFENGVVYAPERAWSETVISECEVFPKGTHDDLVDTVTQALWFLRKSRIALLGD
                     ERASDGRERQRVPGDPRRVPIYDV"
     gene            1953..4766
                     /locus_tag="VGP50_08000"
     CDS             1953..4766
                     /locus_tag="VGP50_08000"
                     /inference="COORDINATES: protein motif:HMM:NF037911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027359.1"
                     /translation="MMAARYARQRLDGHAVVNRQTGARIGLDWERGLKNATAPGMPPA
                     LLLAVPAIPAMLAQARYLRALVPRPPYPPHVLRYRAFAARAEIGGRRVDAILIVQEDR
                     QHRMYLDRVLGGEGPPRRDAGGANPDAPSSTMGPADDAAGADWMAVAPRAPSIGALPF
                     GAGDWAEPIPLSPVDWPRGEESPTNDLVDRFMRRPTPEEAEKFLQPLSMASPRVGPGL
                     SSDAAPQPASTAIGPSATDNGDPPPVASSAQDKAPPSPLAPIPLSDIQKPIQFRGADY
                     EAMGQRLRYLNGKYDPQSWGDQITSAFTGGLNDPSRVMTDSERAEQAALKDKLNQVIK
                     DHYDPQPTPDEARLDNMASSPLGTAAWLGARALGASQQQQDATLGTVGALENIGTAAV
                     GPRTPVEPVLNVPAVAAARAAVATPETLSRTAPPQDGEGMASSQPITSLQAPAAGAQD
                     PERDTLASSELRSSSGGGSGGRSRLYDRLDDFKDFRHDGAYADDPLSQNNAARDYVIQ
                     RQQDIGAEHFIALDTRANDATHAVTSLRQYGTAMPEDLEDAMRDPNAQFNLHHSHSQN
                     TALSDSDVSQLGLPGARWVVAHASAGDISAARLTPEAKAALGSAEKAGQDRARDLLGD
                     LWNKAYDVADSPIQKAVDRGEFTEAEGARIKTEIANRALADSGVTEYVTTHSLPSHPL
                     VDEMQQLANTQMRQDVGQQIPELANVHSPQTRQLGIGEAMARISRGDADAAAGRPGRA
                     TGGRSGTGRAGRPRRQEDPRRDQRAAGRPDSGKYEVYPREPGEGAGGEAREEVAPRIA
                     RASGREFEMPPTSYGHLIILAARYARLRLDGHAVVNRETRVPIALTWERGLKEIVAPG
                     TPPEMLLAVPAIPAMLAGARYLGSAPDPLHRPDIANVHGFASAVEVGGRRIDVLMVVR
                     ENRQGRLFLDRVERRAAAGARAA"
     gene            5203..7896
                     /locus_tag="VGP50_08005"
     CDS             5203..7896
                     /locus_tag="VGP50_08005"
                     /inference="COORDINATES: protein motif:HMM:NF037911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027360.1"
                     /translation="MMAARYARERLDGHAVVNRQTGARIGLDWERGLKNATAPGLPPM
                     LLLAVPAIPAMLAAARYLGALAPRPPYPPHVLRYHAFAAGVEVGGRRVDAVLIVQEDR
                     QHRLYLDRVLGGEARPQRDAGGANPDAPSSTMGPADDAAEVDGPAAPVSTAAAPSAGD
                     NATSAPPPLPQNEGWFHSLLASVGLAGSGDKGPPAPTAPSDDQKSVNYPPPPPPKPAF
                     SRVPDFDEQGFPNYLPGDPYPRYEPTRPPPEPGIGERAWDGFEEGLRQSDTEDVASKA
                     FNYVTTPIRAGFGAVEGALDMPQGAIMDVLPGIVGADAPHVPEAAPPAVLENIAEGKP
                     AAAAGPETMSRTAPPQDGERTATPLSTTPSEGPLPVGAPRVDTSSTAPPLNELGVAAR
                     ERPSSGSVGSDAAPEGPPTALRGPSGEESTQGSLPYYDRLDDFEQYRREAGRDGSDPL
                     SVHGAARAYVMRRQVEGKAEHIAAYDADANAITHVGTSLEKDEVSLPPGLEPKLTDPD
                     ARITLHHSHSDSAGLSRGDISVFSYPGVGWIAGHGIGDELAAARLTPIAREALARPDE
                     AGQRRAQNILQNLWDRAEAACRAPMDEAVGRGEFTEEEGRRIRMEVINRGLADAGVTE
                     YVTSHSLPSRPVVEQMKALANAQLRMNVPRTIPELANYVPDSRTEPVGIRQGMARISR
                     EDDEAAAKRPVRAGGGRISAGRTWHSQRQDYTRGGDGSASGTYGRSYQVPSGAPSRTA
                     QDETGQEVRPSVPRASGADLEMPPTSYGHLIILAARYARLRLDGHAVVNRETRVPIAL
                     TWERGLKEIVAPGTPPEMLLAVPAIPGMLAGARYLGSAPDPLRRPDIANVHGFASAVE
                     VGGRRIDVVMVVRQNRQGRLFLDRMERCAVAGARAAQVLMV"
     gene            8255..>10101
                     /locus_tag="VGP50_08010"
     CDS             8255..>10101
                     /locus_tag="VGP50_08010"
                     /inference="COORDINATES: protein motif:HMM:NF037911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027361.1"
                     /translation="MSSPVAARVAVNALGMAPRNYGHLVMMAARYARERLDGHAVVNR
                     QTGAAIKLDWERGLKNATAPGMPPLLLLAVPAIPAMLAAARYLRALAPRPPHPPHVLR
                     YHAFAATAEIAGRRIKAVLIVQEDRQHRLFLDRVLGGEVPPQWDVGGANPATPNPIPA
                     ADAAASAVATTAPLGEQSIAGSAQKSAMALPAAARVAGDALGMTPRSYGHLIQMAARY
                     ARFRLDGRSVVNHETRAPIMLAWSRGLETGMPPVLMLAVPAIPAMVAAAHYIGAFTRP
                     SPPPDVVRYHAFATAVDVAGRRVNVALIVRENRRGMLFLDRAIGHVAVPRRQQGGANP
                     NDTSVAPPGAPQDIAPTENSGDDNSADDHNPAEDNGASGERTATAGAVAPLAASNGDD
                     HDWLTRQVIGANDWFSRQTQPLRDFLGNLRAPPGPNDPPPTPKDELGLFPSRVFPGGD
                     APTKADGTIVETPDRKAASDALEKRRQELATSGVDPKTDPEYRRLQVAKSRADMNDID
                     DAALMFTPFAGGKGLAGLVERGMSRIGGAGEGAPIAGSAATTPLAKVPPAGELPPSSS
                     GVKDATPAATTPSTPPPSGGAPAPRLASKNAAGVPSAQDLERAIGDLPE"
BASE COUNT         1811 a         3259 c         3492 g         1539 t
ORIGIN      
        1 gcggctgcgc ggatgaagtc gagttcctgt gcggtcaaga ttgaccacgg aggcacggag
       61 agagcacgga gaagcgaagg atggcgcact tcgcgcgccg aacccctctt ctccgtgtcc
      121 gccgtgcctc cgtggtgaac cccatatgcg atagccctcg gggagagggg gatcgtgcgc
      181 gacgcatcga cagaggaggc gtccgatgcg tggcggaagc tcctcggcga cggaggtgcc
      241 cgagagaggg cagatcgtca aagcgctctc tcaggccgat gccgaagaga gcctgctcga
      301 tttcgtgcgg ctgacctggc atgtgatcga gccgtcgcgc gacttcgtcg agggctgggt
      361 catcgaagcg atctgcgatc acctcgaggc cgtgagcaac ggcgagatca gccgcctcct
      421 catcaatgtg ccgccgggct tcacgaagtc cttcctgacg gatgtgttct ggccggcctg
      481 ggaatggggg ccgcgcgacg agccgacgct gcgctatctc tgcttctcct attcggacgc
      541 gctcaccaag cgcgacaacg gccggttcaa gaacctcatc acgtcggagg tctatcgcac
      601 gctctggggc gaccgcttcg ggccgggtga gcagttcggg cggcgcaaga tcgagaacga
      661 ccgcacgggc tggaaattcg cgagctcggt cggcggcgtc ggcaccggcg agcgcggcga
      721 ccgcgtcatc atcgacgacc ccaacaacgt caagaaatcg gaatcgcggg cggtgctgga
      781 gagcaccaat ctgtggctca ccgaagtggt gcccgaccgg ctcaacgacc tcgcgacctc
      841 ggccatcatc ctgatccagc agcgcaccaa cgagcgcgac tgcagcggca cgctgctcga
      901 tccgaagctc ggcgccgagt atgtccatct ctcgatcccc aacgaatacg atccgcgccg
      961 gcattatgtg aatgcgctgg gctggagcga cccgcggagc agcgagggcg cgctcgcctg
     1021 gcccgagcgg ctgcctgcgg ccgagaccga gaagctcagg cgcatcaagg gctcctacgc
     1081 ttattcgggg caataccagc aatcgccggc gccgcgcggc ggcggcataa tcaagcggct
     1141 gcactggcag ctctggccgc gcggcggcga gagcttcgat gcgaatggca agccggcgaa
     1201 gccgctcgaa tatccgccct tcgactatat cgtcgcgtcg ctcgacacgg cgctcggtgc
     1261 gaaagaggag aatgactggt cggcgctgac ggtgtggggc tcgtggcgcg acgcctacga
     1321 cgtgccgaag ctgatgctga tggaggcgtg gcaggcgcgg ctcgaattct atccgctggt
     1381 ccagaagatc atcaagagct gtcgcaagcg ccaggtggac cggctgctgg tcgaagccaa
     1441 ggccaacggc atctcggtgg cgcaagagat cgtccggctc ctcggcgagg aggagttcgg
     1501 cgtgacgctg atcgatccga agaaggccgg cggcgacaaa gtggcgcgcg cctatgcggt
     1561 gcagcatttg ttcgagaacg gcgtcgtcta tgcgccggag cgggcgtggt cggagacggt
     1621 gatcagcgaa tgcgaggtct tccccaaagg gacgcatgac gatctcgtcg acaccgtgac
     1681 gcaggcgctg tggttcctgc gcaagtcgcg catcgcgctg ctgggcgacg agcgcgcgag
     1741 cgacggccgc gagcggcaga gggtgccggg cgacccgcgg cgggtgccga tctacgacgt
     1801 ctgagtgcgc ggccgccgca cgcgccagcg atatcgatcc gaaaattcgg ttttccacaa
     1861 gcataggagc gagcgccatg tcttcgccgg tcgcggcgcg cgtcgccgtc aacgcgctgg
     1921 gcatggcgcc caggaattat ggccatctcg tgatgatggc ggcgcgctac gcgcggcagc
     1981 ggctcgacgg ccatgccgtc gtcaatcggc agaccggcgc gcggatcggg ctcgactggg
     2041 agcgcggcct gaagaatgcg acggcgccgg gcatgccgcc ggcgctgctg ctggcggtgc
     2101 cggcgatccc cgcaatgctg gcgcaggcgc gctatctccg tgcgctcgtg ccgcggccgc
     2161 cctatccgcc gcatgtgctg cgataccgcg cgtttgcggc cagggcggag atcggcggac
     2221 ggcgcgtcga cgcgatccta atcgtgcaag aggaccggca acaccggatg tatctcgatc
     2281 gcgtgctcgg cggcgagggg ccgccgcggc gggacgcggg cggcgcgaac ccggacgcgc
     2341 ccagctcgac gatggggccg gccgatgatg cggcgggcgc ggattggatg gccgttgcgc
     2401 cgcgtgcgcc atcgatcgga gcgttgccgt tcggcgccgg agactgggcc gaaccgatcc
     2461 ctctaagccc agtcgattgg cccaggggtg aagagtcgcc gacgaacgac ctcgtcgaca
     2521 gattcatgcg gcgcccgacc ccggaagaag ccgagaagtt tctccagccc ttgtcgatgg
     2581 cttcgccgcg tgtcggtccg ggtctgtcca gcgacgccgc accgcagccg gcatcgacag
     2641 cgatcggccc ttctgcgaca gacaatggcg atccaccccc tgttgcgtcg tcggcgcaag
     2701 acaaagcgcc gccttcgccg ctcgctccga tcccgctgtc cgacattcag aagcccatcc
     2761 agttccgtgg tgccgattac gaggccatgg gccagcggct ccggtacctg aacggcaaat
     2821 acgatccgca gagctggggc gaccagatca cgagcgcttt caccggcgga ctcaatgatc
     2881 catcgagggt gatgaccgac agcgagcgcg ccgaacaagc cgcgctgaaa gacaagctca
     2941 accaggtaat caaagaccac tacgatccgc agcccacgcc ggacgaagct cggctcgaca
     3001 acatggcatc gagcccgctc ggcacggccg cctggctcgg ggcgagagca ctcggagcaa
     3061 gccagcaaca gcaggatgcg acgctcggga ctgtcggagc attggaaaac atcgggacgg
     3121 cggcggtcgg tccgcgcacg cctgtagagc cggtactcaa cgtgcctgcc gtggccgccg
     3181 cgcgcgcagc cgtcgcgaca ccggaaacgc tgtcccggac ggcgccgccg caggacggcg
     3241 aaggaatggc gagttcgcag ccaatcacat cgctgcaggc gcctgctgct ggtgcgcaag
     3301 atccagagcg ggacacgctc gcgtcgagcg agctccggtc gtcaagcggt ggaggaagcg
     3361 gcgggcgctc ccggctctat gaccgcttag acgatttcaa ggattttcgc catgatggcg
     3421 catacgccga tgatcctcta agccagaaca acgctgcgcg cgactatgtg attcagcgcc
     3481 agcaggatat cggcgccgag catttcatcg ccttagacac tcgtgcaaat gacgccactc
     3541 acgccgtaac ttcgctaagg caatatggca cggcgatgcc ggaagacctg gaagacgcga
     3601 tgcgtgatcc gaacgcgcaa ttcaatttgc atcacagcca ttcacagaat acggcactga
     3661 gcgacagcga tgtttcgcag cttgggcttc ctggggcgcg ttgggttgtc gcgcatgcca
     3721 gtgcagggga tatctcggcg gcccggctga cgccggaggc caaagcagcg ctcggctccg
     3781 ccgagaaagc agggcaggat cgggcgcgtg atcttctggg cgacctgtgg aacaaggctt
     3841 atgacgtcgc cgacagtccg atccagaagg ccgtggatcg gggcgagttc acggaggcgg
     3901 aaggagcgcg tatcaagacg gaaattgcaa accgcgccct tgcagattcg ggtgtcaccg
     3961 agtatgtgac gacgcacagc ttgccgtctc atcctttggt cgacgagatg cagcagctgg
     4021 cgaatactca aatgcgacaa gacgttggcc aacaaatacc ggagctggcg aatgtacata
     4081 gtccacaaac ccgacagctc ggcatcggag aagcgatggc gagaatttct cgaggagatg
     4141 ctgacgctgc cgcaggacga cctggacgtg cgacgggcgg tcgaagcggc acgggacgag
     4201 ctggacgtcc gcgccggcaa gaagacccgc gccgagatca gcgcgcggcg ggacgaccgg
     4261 atagcggaaa atatgaagtt tatccgagag agccgggcga gggagcaggg ggcgaagcaa
     4321 gagaagaagt agctccgcgc atcgcccggg cgtcggggcg agagttcgaa atgccgccca
     4381 cgagctacgg ccatctcatc atcctggcgg cgcgctatgc gcggctgcgg ctcgatggcc
     4441 atgccgtcgt caatcgcgag acgcgcgtgc cgatcgcgct cacctgggag cgcgggctca
     4501 aggagatcgt cgcgccgggg acgccgcccg agatgctgct ggcggtgccg gcgattcccg
     4561 ccatgctcgc cggggcgcgg tatctgggat cggcgcccga tccgctgcac cgcccggata
     4621 tcgccaacgt gcacggcttc gcgtcggcgg tcgaggtcgg cgggcggcgc atcgatgtcc
     4681 tgatggtggt gcgcgagaac cggcaggggc ggttgttcct ggaccgggtg gagcggcgcg
     4741 cggccgcggg tgctcgcgcg gcgtaattct cgatcgtctg gcgccgcggc ggcggcaccc
     4801 ctcaccctca ctgcttcgct aaagcttcgc agtgtttccc cactgcttcg ctaaagcttc
     4861 gcagtgggtc cctccctctc ccgcaagggg agaggggaag aagtcaatcg tcaataacct
     4921 cgtcgatgat gtaatccagc gctgtggttc ctgtgcaagt cgcgcatcgc ttttttgcgc
     4981 gaggagcgcg cgagcgatgg gcgcgcgcgg caaagggcgc caagcgatcg gccgggtcgt
     5041 cgatctacga cgtccgatgc gacgcggacg cggcggcgaa ggccatccga caatccggtt
     5101 ttcaacgagc tcaggagagc gagcgccatg tcttcgccgg tcgcggcgcg cgtcgccgtc
     5161 aacgcgctgg gcatggcgcc gaggaattat ggccatctcg tgatgatggc ggcgcgctac
     5221 gcgcgggagc ggctcgacgg ccatgccgtc gtcaatcggc agaccggcgc gcggatcggg
     5281 ctcgactggg agcgcggcct gaagaacgcg acggcaccgg gcttgccgcc gatgctgctg
     5341 ctggcggtgc cggcgatccc ggcgatgctg gcggcggcgc gctatctcgg cgcgctcgcg
     5401 ccgcgaccgc cctatccgcc gcatgtgctg cgctatcacg cgttcgcggc cggggtggag
     5461 gtcggcggac ggcgcgtcga cgcagttctg atcgtgcaag aggaccggca gcaccggttg
     5521 tatctcgatc gcgtgctcgg cggtgaggcg cggccgcaac gggacgcagg cggcgcaaac
     5581 ccggacgcgc ccagctcgac gatggggccc gccgatgacg cggcggaggt ggatgggccg
     5641 gcggcgcccg tgtcgacggc ggcggcccct tcggccggag acaatgccac ttcagcgcct
     5701 ccgccgttgc cgcaaaatga gggatggttc cattcgctgc tcgcatcggt cggacttgca
     5761 ggctcgggcg acaaaggccc cccggcgccg accgcgccgt ccgacgatca gaaatctgtc
     5821 aactacccgc cgcctccgcc gcccaagccc gcatttagcc gcgtcccgga tttcgacgaa
     5881 cagggtttcc caaattatct gccgggcgat ccttacccga ggtatgagcc gacaaggccc
     5941 ccgcccgagc caggcatcgg cgagcgcgca tgggacggat tcgaggaagg cctgcgccag
     6001 tccgacacgg aagacgtcgc cagcaaggcc ttcaactacg tcacgacgcc aataagggcc
     6061 ggcttcggcg ctgtcgaggg cgcgctcgac atgccgcaag gggcgatcat ggacgtgctg
     6121 cccgggatcg tcggtgcgga cgcgccgcat gtgcctgagg cagcgccgcc cgccgtcctc
     6181 gagaatatcg ctgaaggcaa gcctgcggct gccgctggtc cggaaacgat gtcccggaca
     6241 gcgccgccgc aggatggcga aagaacggcg acgccgctgt cgaccacgcc ttccgaagga
     6301 ccactgcccg tcggggcacc gcgggtcgac acctcatcca cggcgccgcc gctcaacgag
     6361 cttggggtcg ccgcgcggga acggccgtcc tcaggttcgg tcggctccga tgcggcgccc
     6421 gagggaccgc cgaccgctct tcgcgggccg tccggcgaag aatcgaccca gggatcgctc
     6481 ccatattatg accgcctcga tgacttcgag cagtatcgac gggaggcagg acgtgacggc
     6541 agcgatccgc tgagcgttca tggagctgcg cgcgcttatg tgatgaggcg ccaagtcgaa
     6601 ggtaaagccg agcacatcgc ggcttatgac gccgatgcga atgcaatcac ccatgtcggc
     6661 acttcgcttg aaaaggatga ggtgagcctc cctccggggc tcgaacccaa gctgaccgac
     6721 cctgatgcga gaattacgct ccaccatagt cactcggaca gcgcgggctt gagcagggga
     6781 gacatcagcg ttttctctta tcccggcgtc ggttggatcg ccggtcatgg catcggcgac
     6841 gagctcgcag ccgctcgact gacgcccata gcgagagagg cgctggcgag gcccgatgaa
     6901 gccggccaac gtagagcgca gaacattctg cagaacctct gggaccgagc tgaggctgcg
     6961 tgtcgcgctc cgatggacga ggctgtgggg cgcggtgagt tcacagagga ggaagggcgc
     7021 cggattcgca tggaggtcat caaccgtggc cttgcggatg caggggtgac ggaatacgta
     7081 acgtctcaca gcctgccttc gcgtcccgtg gttgagcaga tgaaagcgct ggcgaatgcc
     7141 caactgcgga tgaacgttcc cagaacaata ccggagctag ccaactatgt acctgattca
     7201 cgaaccgagc ccgtgggcat ccgacaagga atggcaagaa tttctcgcga ggatgatgaa
     7261 gctgccgcaa aacgaccggt tcgtgcaggc ggcggtcgaa ttagcgcagg acgaacttgg
     7321 cattcgcagc ggcaagatta cacgagagga ggtgatggct cggcgtcagg cacgtatgga
     7381 cgaagctatc aagtaccttc gggagcaccc agccgaactg cgcaagacga aacaggacaa
     7441 gaagtaaggc cgagcgtccc gcgggcgtcg ggggccgatc tagaaatgcc ccccacgagc
     7501 tacggccatc tcatcatcct ggcggcgcgc tatgcgcggc tgcggctcga tggccatgcc
     7561 gtcgtcaatc gcgagacgcg cgtgccgatc gcgctcacct gggagcgcgg gctcaaggaa
     7621 atcgtcgcgc cggggacgcc gccggagatg ctgctggcgg tgccggcgat tcctggcatg
     7681 ctcgccgggg cgcggtatct gggatcggcg cccgatccgc tgagacgccc ggatattgcc
     7741 aatgtgcacg gcttcgcgtc ggcggtcgag gtcggcggac ggcgcatcga tgtggtgatg
     7801 gtggtgcgcc agaaccggca ggggcgcttg ttcctggacc ggatggagcg gtgcgcggtc
     7861 gcgggggcgc gcgcggctca ggttttgatg gtctgacgcc gcgggctgcg gcaccctcac
     7921 cctcactgct tcgctgaagc ttcgcagtgt ttccccactg cttcgctaaa gcttcgcagt
     7981 gggtccctcc ctcttccacc ccttcgacaa gctcaggagg gagaggggaa gaagtcgatc
     8041 gtcgataacc tcgtcgatga tgtaacccag ctccgtggtt cctgcgcaag tcgcgcatcg
     8101 cgctgctcgg cgacggccgc gagcggcaga gggtaccgcg cgccgccgcg tcggcatcca
     8161 tcgttcctcg aaaatacctt tcgacaaact catccttcga caggctcatc cttcgacagg
     8221 ctcatccttc gacaggcgca ggagagcgag cgccatgtct tcgccggtcg cggcgcgcgt
     8281 cgccgtcaac gcgctgggca tggcgcccag gaattatggc catctcgtga tgatggcggc
     8341 gcgctacgcg cgggagcggc tcgacggcca tgccgtcgtc aatcggcaga ccggcgcagc
     8401 gatcaagctc gactgggagc gcggcctgaa gaatgcgacg gcgccaggca tgccgccgct
     8461 gctgctgctg gcggtgccgg cgatcccggc gatgctggcg gcggcgcgct atctccgcgc
     8521 gctcgcgccg cgaccgcccc atccgccgca tgtgctgcgc tatcacgcct tcgcggcgac
     8581 ggcagagatc gccggccgcc gcatcaaggc cgtcctgatc gtgcaagagg accggcaaca
     8641 ccggttgttt ctcgatcgcg tactcggtgg cgaggtgccc ccgcaatggg acgtcggtgg
     8701 cgcaaacccg gcgacgccca atccgattcc agcggccgat gctgcggcaa gcgcggttgc
     8761 cacgaccgcg ccactcggcg agcaatcgat tgccggctcg gcgcagaaga gcgccatggc
     8821 tttgccggct gctgcgcgcg tcgctggcga cgcgctgggc atgacgccga ggagctacgg
     8881 ccatctcata cagatggcgg cgcgctacgc gcgctttcgg ctcgatgggc gcagcgtcgt
     8941 caatcacgag acgcgtgcgc cgatcatgct tgcctggtcg cgtgggttag agaccgggat
     9001 gccgccggtg ctgatgctgg ccgtgccggc gattccggcg atggtcgcgg cggcgcacta
     9061 tatcggcgcg ttcacccgcc cgtcgccgcc gccggacgtg gtgcgctatc acgccttcgc
     9121 gactgcggtc gacgtcgctg ggcggcgggt caatgtcgcg ctcatcgtgc gcgagaaccg
     9181 gcgaggcatg ctcttcctcg accgggcgat aggccatgtg gccgtaccga ggcggcagca
     9241 gggcggcgcc aatccgaatg atacgagcgt ggcgccgccg ggcgcgccgc aagacatcgc
     9301 tccgactgaa aacagcggcg atgacaattc cgccgacgat cacaatcccg ccgaagataa
     9361 cggtgccagc ggcgaacgga ccgccaccgc gggcgccgta gcaccgctcg ctgcaagcaa
     9421 cggcgacgac catgactggc tgacgcggca ggtcatcggc gcaaacgatt ggttcagccg
     9481 gcagacgcag ccactgcgcg atttcctcgg caatctcaga gccccgccgg ggccgaatga
     9541 tccaccgccc acgcccaagg acgagctggg cctcttcccg tcgcgcgtct tcccaggggg
     9601 cgatgcgccg accaaggccg acgggacgat cgtcgaaacg cccgaccgaa aggccgcatc
     9661 cgacgcgctg gagaagcggc gacaggagct ggcaacgtcc ggcgtcgatc cgaagaccga
     9721 ccccgagtat cgccggctgc aagtcgcgaa gtcgcgcgcc gacatgaacg acatcgatga
     9781 tgcggcgctg atgttcacgc cgttcgccgg cggcaagggg ctggcaggac tcgtcgagcg
     9841 gggcatgtcg cggatcggcg gcgcaggcga aggggcgccc atcgccggat ctgccgcgac
     9901 gacgccactc gccaaggtgc cacccgccgg cgaattgccg ccgtcgtcct ccggcgtgaa
     9961 ggatgcgaca cccgctgcca cgaccccgag tacgcccccg ccttccggcg gagcaccagc
    10021 gccgcgtctc gcctcaaaaa acgccgcggg tgtccccagc gctcaagatc tggagcgggc
    10081 gataggcgac ctgccggagg a
//
LOCUS       DASYNS010000255         3268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_839393, whole genome shotgun sequence.
ACCESSION   DASYNS010000255 DASYNS010000000
VERSION     DASYNS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839393"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..2255
                     /locus_tag="VGP50_08015"
     CDS             105..2255
                     /locus_tag="VGP50_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV8027362.1"
                     /translation="MVRSIASRRGLGIRGKLRSAFIAVAGLTVVASGYAYWSYEDMGQ
                     TLDNIADRNLPAMSLSLQLARSSAEIVSVAPKLLAAADLDERKKALAKLDENQRELTR
                     AIDALAATPGGREDTASLRGAADQMAANLAELARNVEQRLALRQQRLSTDAGVRATYG
                     TLSQALAPMIDDAYFDLRRALATASEAPDAKTMKAHLAELTGGKFATLQSLSDIRADS
                     NLVLGLLTEAANLPSEDLMPPLHDRFKATAHHLEQSLEQLKNDGAAKTLSGPTSELMS
                     YGQGNTTIFDVRGRELATLAAGEALLATNGDLARNLEQEVSTLVDRSEGAAKAAATST
                     GTMIAQGRIVLIATAVASVLVAIAIGVFYVGGSVMRRLGLVQRSMAELAAGNLDAAIP
                     QGGADEIAEMASALLVFRDTGRAAKSADDVASAERRKNAEQRRIDLLSLADGLESSVK
                     SVVETVSSAASAMQSTAGNMATTAEETSHQAAAVSDASAAASGNVHSVAAAADELSRS
                     TAEIGQRILESARVAGRAVRETQRTTTTMQGLAASARKIGDVVQLINVIASQTNLLAL
                     NATIEAARAGEAGKGFAVVASEVKTLANQTAKATDEIATQIREIQDATNGAVGAIEEI
                     SQTIARVNEIATAVAAAVEQQEATTRNIAHSVQQAAEGTASVSANIAGVTRSASETGQ
                     AAHMVLKSAGDLAAQAERLRYGVDRFLSGVRATR"
     gene            2571..3152
                     /gene="pdxH"
                     /locus_tag="VGP50_08020"
     CDS             2571..3152
                     /gene="pdxH"
                     /locus_tag="VGP50_08020"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043743428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEV8027363.1"
                     /translation="MAATPEPIEQFARWFAEAAAKEPEPNAMSVATVAAGGAPSARMI
                     LLKGVDDRGFVFYTNLDSRKAAELSENPHVALCFHWKSLGRQVRVEGVVEAVSEREAD
                     AYFATRPRESQLGAWASDQSRRLASRSVLDERVAAVEKRFAHGPIPRPPHWSGYRVLP
                     SRVEFWEAQPFRLHDRLVYVRDGNAWRSERLYP"
BASE COUNT          561 a         1123 c         1144 g          440 t
ORIGIN      
        1 cggctcgaac caggtgttcc taaccgtcat ccagccggac ggcaagctgc atccggtcgc
       61 gcggctcgcg aaggcgagcg gctgagtaag gagagagcaa gctcatggta cgcagtatcg
      121 cctcgcgccg cggccttggc atccgcggca agttgcggtc cgccttcatt gccgtcgccg
      181 gcttgaccgt cgtggcgagc ggctacgcct actggtccta tgaggacatg gggcagacgc
      241 tcgacaacat cgccgaccgg aacctcccgg cgatgagcct ttccttgcag ctggcgcgaa
      301 gcagcgccga gatcgtctcg gtcgcgccga agctgctggc cgccgcagat ctcgacgagc
      361 gcaagaaggc gttggcgaaa ctcgatgaaa atcagcgaga gctgacccgc gccatcgacg
      421 ctctcgcggc gacgccaggc ggccgtgaag atacggcatc gctgcggggt gctgcggacc
      481 agatggcggc gaaccttgcc gagctggcga ggaacgtcga gcagcggctc gcgttgcgcc
      541 agcagcgcct gtccaccgac gctggcgtcc gcgcgaccta cggcacgctc tctcaggcac
      601 tggcgccgat gatcgatgac gcctatttcg acctccgtcg cgcactcgcg accgcgagcg
      661 aggctcccga cgccaagacc atgaaggcgc atctcgcgga gctcaccggc ggcaagttcg
      721 ccacgctgca aagcttgtct gacatccgcg ccgacagcaa tctcgtgcta gggctgctca
      781 ccgaggcggc caacctgccg agcgaggatc tgatgccgcc gctgcacgac cgcttcaagg
      841 cgacggcgca tcacctcgag caatcgctcg agcagctcaa gaatgacggc gccgcaaaaa
      901 cgctcagcgg cccgaccagc gagctgatga gctacggcca aggcaacacc accatcttcg
      961 atgtccgcgg acgagagctg gcgacgctgg cggccggcga agcattgctc gcgaccaacg
     1021 gagacctcgc ccgcaatctc gagcaggagg tgtcgacgct ggtggatcgc agcgaaggcg
     1081 ccgccaaggc cgccgcgacc agcaccggga cgatgatcgc gcagggacgc atcgtgctga
     1141 tcgcgaccgc ggtcgccagc gttctcgtcg cgatcgcgat cggcgtcttc tatgtcggcg
     1201 gcagcgtcat gcgccggctt gggctcgtgc agcgcagcat ggcggaactc gccgccggca
     1261 acctcgacgc cgcaatccct cagggtggtg ccgacgagat cgccgagatg gcctcggcgc
     1321 tcctcgtgtt ccgtgatacc gggcgggcgg ccaagtctgc cgacgacgtg gcctcagcgg
     1381 agcggcgaaa gaatgccgag cagcgccgga tcgacctgtt gtcgctggcg gacggcctcg
     1441 agagcagcgt caagagcgtg gtcgagacgg tgtcgagcgc ggcgagcgcc atgcagagca
     1501 cggccggcaa catggcgacg accgccgagg agaccagcca tcaggccgcc gccgtgtccg
     1561 acgcctccgc cgcggcatcg gggaacgtgc acagcgtcgc cgcggcagcc gacgagctgt
     1621 cgcgctcgac cgcagagatc ggccagcgca tcctggaatc ggcgagggtt gccggccgcg
     1681 ccgtgcggga gacgcagcgc acgacgacga cgatgcaggg cctcgcggcg tcggcgcgca
     1741 agatcggcga cgtggtgcag ctcatcaatg tcatcgcctc gcagaccaac ctgctggcgc
     1801 ttaacgccac catcgaggcg gcgcgcgccg gcgaggccgg caagggcttc gcggtggtcg
     1861 cgtcggaagt gaagacgctg gccaatcaga ccgcgaaagc gaccgacgag atcgccacgc
     1921 agatccgcga gatccaagac gcgacgaatg gcgccgtcgg cgccatcgag gagatttcgc
     1981 agaccattgc ccgcgtcaac gagatcgcga ccgcggtcgc cgcggcggtc gagcagcagg
     2041 aagcgacgac gcgcaacatc gcgcacagcg tccagcaggc ggccgaaggc acggccagcg
     2101 tctcggcgaa catcgccggc gtgacgcgga gcgccagcga gaccggacag gcggcccata
     2161 tggtgctgaa atcggccggc gacctcgccg cgcaagcgga gcggctgcgc tacggcgtcg
     2221 accgcttcct gagcggcgtc cgcgcgacac gctaggcagc gcgacgcgca gcgtcgtcgc
     2281 tggcgggcgg cagcggttgc gaagcaaccg cgagagcccg caccgagcag acgagccgat
     2341 gtcgtcgacc ttcgaaccgc tgtcgcccgc tcgcgacaac cgctacgcgg ttgcgcgggg
     2401 cactggcgcg gaccgcagat attggctaat ccgcagggag cgggctttcg cggttgctgg
     2461 cgcaaccgct gtcgcccgct cgcgacaacc gctacgcggt tgcgcggggc gcaaccgctg
     2521 gcgcccgccg cggacgaagg cgcttcgcgc cttcgccaag aggaccgagg atggccgcca
     2581 cgcctgagcc gatcgagcag ttcgcccgct ggtttgccga ggccgcggca aaggagcctg
     2641 agcccaacgc catgagcgtc gcgacggtgg cggcgggcgg cgcgccctcg gcgcggatga
     2701 tcctattgaa aggcgtcgac gaccggggct tcgtcttcta caccaatctc gacagtcgca
     2761 aggcggcgga gctgagcgaa aatccccatg tcgcgctgtg ctttcactgg aagtcgctgg
     2821 gccggcaagt gcgcgtcgaa ggcgtggtcg aggcggtgag cgagcgcgaa gcggacgcct
     2881 atttcgcaac gcggccgcgg gaaagccagc tcggcgcctg ggcgtccgac cagtcgcggc
     2941 ggctggcgag ccgctcggtt ctcgatgagc gcgtcgccgc cgtggagaag cgcttcgccc
     3001 acggacccat tccacggccg ccgcattggt cgggctaccg cgtgctgccg tcgcgggtcg
     3061 agttctggga ggcccagccc ttccgcctgc atgaccggct cgtctatgtc cgtgacggca
     3121 atgcctggcg cagcgagcgg ctctacccat gacggccgac caagccgccc cgtcgccggc
     3181 cgtcacgggc ctcctcatgc gccgcgccac gcgcgcatcg atcgccgtcg ccgtcgtgat
     3241 ggtggcggtg aagctcggcg cctggctg
//
LOCUS       DASYNS010000256         3581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2402017, whole genome shotgun sequence.
ACCESSION   DASYNS010000256 DASYNS010000000
VERSION     DASYNS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402017"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VGP50_08025"
     CDS             complement(<1..181)
                     /locus_tag="VGP50_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027364.1"
                     /translation="MSRQDLLSRVRRGLAHILSPDEEVKVPARKARPAAAAGNDLDAS
                     VNFAMQLKKLFAEGSF"
     gene            complement(293..421)
                     /locus_tag="VGP50_08030"
     CDS             complement(293..421)
                     /locus_tag="VGP50_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027365.1"
                     /translation="MKAARLRELAKVHGDDPVGRKLWRLAQDMDTLALDRERRLAP"
     gene            907..1137
                     /locus_tag="VGP50_08035"
     CDS             907..1137
                     /locus_tag="VGP50_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027366.1"
                     /translation="MAMPGHPSLDPSTEVEFAYFMLAMIVAGVLIYALYTETFPLRVR
                     GINRAEEPFSYWGIFALYFGMFVMAVVKSLGF"
     gene            1206..1493
                     /locus_tag="VGP50_08040"
     CDS             1206..1493
                     /locus_tag="VGP50_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027367.1"
                     /translation="MPARRSLKYLFTELPQLRWHERFWVGLPFGLVLVGGAMGGACGA
                     AAAACNLRLMRGERPLFQKYLLTALISLAAVVAYVALAALFLSLAGRRAAA"
     gene            1631..2623
                     /locus_tag="VGP50_08045"
     CDS             1631..2623
                     /locus_tag="VGP50_08045"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8027368.1"
                     /translation="MQHILVIGGEPAVSHLVRSALQQDGRYRVTQASSSKEALPLLGH
                     DRPSLAIIDSSLPDMTGIELAERARRQDVPVLLLTDRPAAERADRGFVTLGKPIVLDD
                     LLQRSRALIAEAKQQHHLLRESLRRLKAGCGGVYGALKQASATIAQISGERSDTGRGD
                     KPPYDFRSDPILHEALAYWREKRATRAMPMRRDIDPAELPKHLLPHLLLIDVVDGGER
                     FHFRLAGTAIVDAFGAEFTGKHADELIKAERIAHLHRYYRMVIEARRPVLIRSRYLTT
                     KAVDMTANRLLLPLSQDGVQVNMILGALTFEFMGACATERGEPPDETTTYIDVA"
     gene            2731..3252
                     /locus_tag="VGP50_08050"
     CDS             2731..3252
                     /locus_tag="VGP50_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027369.1"
                     /translation="MPLVPQDLDEVRTLCAARRHDDALKLCGEFIAENRDHPHGYHMR
                     AVVRVLMGEPHLALADRDKVVSLCPREPGAYMARADDEIRLRDFAAAAADLNRAEKLD
                     DGHYWPMIPLLRAQCRARLGHFADALADLARVPDDYLLPGFGADAAGSKRRVMAEITE
                     LQELAAANEDSQA"
     gene            complement(3359..>3581)
                     /locus_tag="VGP50_08055"
     CDS             complement(3359..>3581)
                     /locus_tag="VGP50_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dicarboxylate--CoA ligase PimA"
                     /protein_id="HEV8027370.1"
                     /translation="YRGQSAKAFIALKPDTPPFTLEQLKSFLADKLAKYELPTEIEFR
                     PDLPKTPVGKLSKKELVAEEMTKRGKAAE"
BASE COUNT          564 a         1206 c         1142 g          669 t
ORIGIN      
        1 cgaacgagcc ttcggcgaac agtttcttca gctgcatcgc gaagttcacg ctggcgtcca
       61 aatcattgcc cgcggcagcg gccggcctgg ctttgcgcgc gggaaccttc acttcctcgt
      121 cgggactgag gatgtgggcg aggccgcggc ggacgcggga gagcaaatcc tgccgactca
      181 tctgcttcgt ccttcgagca cccggatcac gagcgcaacg cgcgaggctg cgttctttat
      241 ccgcttggaa gatgcgtcgt cgccgacaat cgacatggcg ccgggccgcc gcttatggtg
      301 cgagccggcg ttcccggtcg agagcgagcg tgtccatgtc ctgcgcgagc cgccacagtt
      361 tccgcccgac gggatcgtcg ccatggactt tcgccaattc gcgcaagcgc gcggctttca
      421 tgcgaaggta ttcgggatct atcatcggca tcaggagagg ctagagccgc ggcgttaagg
      481 cgacctttcc cacgcgcgga tttcgccacg cgcggcgcgt tttggtgcgg cgttctcatt
      541 cttcgcttcg ccgatttgcc actgatgcgc aagccccgac cttatcacaa gtgacgaaga
      601 cgcggcggct tcgaacgttc gccccgtctt caatcgtcgc gatttgccga ttgagcgcgt
      661 tcggaagatg acattcgtct cggcgacaga ctgtgccgcc gcggttcatc atcgcttcga
      721 tatccccgca tgggcgcgat gcgctcgcat ggtgcggccg atgatcggga tcggtccctt
      781 cgggtcggct gccgtgcggt ggcgttcccg gcgagcgcgg ggctaacccc atgaaatggt
      841 tcgccgggcg ccctcgggag gaggcaacca agctttaagc ctcgctgaca taaacttgac
      901 actgacatgg caatgcccgg gcacccttcg ctcgacccgt cgaccgaggt cgagttcgcc
      961 tatttcatgc tggcgatgat cgtcgcgggc gtcctcatct atgcgctcta tacagagact
     1021 tttccgctgc gcgtgcgcgg catcaaccgc gctgaggagc ccttctccta ctggggcatc
     1081 ttcgccctct acttcgggat gttcgtcatg gccgtcgtca agtcgctggg cttttagccc
     1141 ttggcgccgc gacggtaaca cttcgttagc cacgcggctg ctagcgtcgc cgcgggaggg
     1201 cgatcatgcc cgcgcgacgc agccttaaat atctcttcac cgagctgccg cagcttcgct
     1261 ggcatgagcg attctgggtc ggcctgccct tcggcctcgt cctggtgggc ggcgccatgg
     1321 gcggcgcctg cggcgccgcg gccgcggcct gcaacttgcg gctgatgcgc ggcgagcgac
     1381 cccttttcca gaaatatctc ctcaccgcgc tgatctcgct cgccgcggtg gtcgcctatg
     1441 tcgcgctcgc cgcgctcttc ctgtcgctcg ccggccggcg tgccgccgcg tgaccatcga
     1501 gcacccatta tctcccttga gggctcgagc ccgttttttt gccacttacc ctttgctcta
     1561 tttcagggta ttcagcgata gtgtttcagg tacgagagcg ctttcctcgc ccgctacgcg
     1621 ggagggtctc gtgcagcata ttctggtgat cggcggcgag ccggccgtct ctcatctcgt
     1681 gcggtcggcc ctgcaacaag acggtcgata cagagtgacg caggcgtcct cgagcaaaga
     1741 ggcgctgccg ctcttgggtc atgaccgtcc gagcctcgct atcatcgatt catcgctccc
     1801 ggacatgacg gggatcgagc tcgccgagcg ggcgcggcgg caggacgtgc cggtcctgct
     1861 gctcacggac cggccggcgg cggagcgcgc cgatcgcgga ttcgtcacgc tgggcaagcc
     1921 gatcgtgctc gacgatctcc tccagcgcag ccgagcgctg atcgcggaag caaagcagca
     1981 gcatcatctg ctccgcgagt cgctgcggcg gctcaaggcc ggctgcggcg gcgtctacgg
     2041 cgcgctgaag caggcgagcg cgacgatcgc gcagatcagc ggcgaacgca gcgatacagg
     2101 gcgcggcgac aagccgccct atgatttccg cagcgacccc atcctgcacg aggccctcgc
     2161 ctattggcgc gagaagcgcg ccacgcgcgc catgccgatg cggcgcgaca tcgatccggc
     2221 ggagctgccg aagcatctcc tgccgcatct gctgctcatc gacgtcgtcg atggcggcga
     2281 gcgcttccat tttcgtctcg cgggcacggc gatcgtcgac gccttcggcg ccgagttcac
     2341 cggcaaacat gccgacgagc tgatcaaggc cgagcgcatc gcccatctgc atcgatacta
     2401 tcgtatggtg atcgaggcgc gacggccggt ccttatccgc agccgctatc tcaccaccaa
     2461 ggccgtcgac atgaccgcga accgcctgct cctgccgctc tcgcaagacg gcgtgcaggt
     2521 caacatgatc ctgggcgcgc tcaccttcga gttcatgggc gcctgcgcca cggagcgcgg
     2581 cgagccgccc gacgagacga cgacctatat cgacgtcgcc taaaattcca gtctctaaaa
     2641 tcccgagcga agagctgcgc ggcgtcctcg acagccggcc ctccgaaggc gcacaatccg
     2701 cgcctcgcgt ttgccgcgca aggagccgcg atgccgctcg tcccccagga tctcgacgaa
     2761 gtccgcacgc tctgcgcggc acggcgccat gacgacgcgc tgaagctctg cggcgagttc
     2821 atcgccgaga accgcgacca tccccatggc tatcacatgc gcgccgtcgt gcgcgtgctc
     2881 atgggcgagc cgcatctggc gctcgcggac cgcgacaagg tggtgtcgct ctgcccgcgc
     2941 gagcccggcg cctatatggc gcgggccgac gacgagatac ggctccgcga tttcgccgcg
     3001 gcggcggccg atctcaaccg cgccgagaag ctcgacgacg gccattactg gccgatgatc
     3061 ccgctgctgc gcgcgcagtg ccgggcgcgg ctcgggcact ttgccgacgc gctggccgac
     3121 ctcgcgcgcg tgccggacga ctacctgctg ccggggttcg gcgccgacgc ggccggctcg
     3181 aagcggcgag tgatggcgga gatcacggag ctccaggagc tggcggccgc gaacgaggat
     3241 tcgcaggcct gaagatcaac ctttcgacgg acgcggctcg agataaatca gcgaacgcag
     3301 aggacgagtc ttcctctgcg tcctccgcgt aacccccgcg ttccttgtct cttgacactc
     3361 actcggccgc tttgccgcgc ttcgtcatct cctcggcgac gagctctttc ttcgagagct
     3421 tgccgacggg ggtcttaggg aggtccggcc ggaactcgat ctcggtcggc aactcatatt
     3481 tggcgagctt gtcggcgagg aaggatttga gctgctcgag ggtgaagggc ggggtatcgg
     3541 gcttcaaggc aatgaaagcc ttggccgact ggccacgata g
//
LOCUS       DASYNS010000257         4568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3494115, whole genome shotgun sequence.
ACCESSION   DASYNS010000257 DASYNS010000000
VERSION     DASYNS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3494115"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VGP50_08060"
     CDS             complement(<1..530)
                     /locus_tag="VGP50_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8027371.1"
                     /translation="MATLTPILRARIAEKRYPGIGGAPAHLALREFVVNVPAGEFVAL
                     VGPSGCGKTTFLNIVAGLDGDFTGELALLPAENGREPRIGYVFQNPRLLPWRTVRENL
                     TLVLRPGQDPGIVDRLLRAVELEAARDVYPGRLSGGMSRRAAIARAFAVDPDLLLMDE
                     PFVSLDEDSAGRLRQLL"
     gene            complement(518..1285)
                     /locus_tag="VGP50_08065"
     CDS             complement(518..1285)
                     /locus_tag="VGP50_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV8027372.1"
                     /translation="MRRMKLPQLGGSILRRTLSLVLLLALWEAVALFAQSRLVPTVGA
                     VFRALAKEVVSGPLPHHLGVTLLRVAAAFFIAMVIGSVIGVVMGRSRRLDAFFDSWLV
                     LLLNLPALVVIVLAYVWFGLTEAAAIGAVAVNKIPTVAVTLREGARALDRDLADVARS
                     FRIGRLSNLRHFVLPQLYPFLLAAARSGLALVWKIVLVAELLGRSDGVGFAIQLHFQQ
                     FDVPEILAYTVAFTAVVQAIEWGILQPLEQRSSRWRR"
     gene            complement(1292..2272)
                     /locus_tag="VGP50_08070"
     CDS             complement(1292..2272)
                     /locus_tag="VGP50_08070"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027373.1"
                     /translation="MRRIAAAGAALVLAVLALAGNARAADPLRVGVLKFGTVSWELDV
                     IRQHGLDTAEGFAVEPVELASTPATLVALQAGSVDTVVSDWFWVSRQRASGADWTFFP
                     FSTAVGALVAAPQSPVRTLADLKGRRLGIAGSAVDKSWLIMRAAAAQQSGLDLERDTD
                     KSFAAPPLLDAELAAGRLDAVLTYWPFAARLEARGMRQIMSVGDAMHALGFESAVPMV
                     GYVVAEHWAAAHRPLLAAFVRASRRAEELLAGSDEEWTRIAPRTGAHDAAELVKLRDA
                     YRAGIPRHWGDAERRDAERLYVLLAKIGGEALAGPVPLLQPGTFLDDVRY"
     gene            2432..3646
                     /locus_tag="VGP50_08075"
     CDS             2432..3646
                     /locus_tag="VGP50_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027374.1"
                     /translation="MLGPYTTRALLALSLFATTLPGSAAGAADEAAPAALRIGFLSVA
                     VKQPAPEPYLDPPPADEGVAGARLGIDDDNTTGRFTGQQFALEEEMVPAEGDVAAGFR
                     RLVGSGIRLVVTDLPKAALLAIADLPDAKDVLLLDAGTEDDELRAEKCRANVLHTLPS
                     RAMLADGLVQYLVAKQWRRVLLVVGPTEADGAYAAAVKRAITKFQAKLVDEKPWTYQP
                     GARRSDTGHYSIAAEVARFTQGISYDVLVVADEDDEFGDYLSYATTDPRPVAGTHGLV
                     AGAWARPHQEWGATQLQDRFLHRAKRWMTDRDYAAWLAVRAAGEGATRAQSVEPAKLT
                     TYMRSDKFELGGYKGLPLSFRPWDGQMRQAILLYDSRALVSVSPQPGFLHQFSTLDTL
                     GLDRPESKCHLP"
     gene            3631..>4568
                     /locus_tag="VGP50_08080"
     CDS             3631..>4568
                     /locus_tag="VGP50_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HEV8027375.1"
                     /translation="MPSAVSKHHRSRAIAALALASALWPAGPLRAESVLVSNEKGNSI
                     TVLDAETLTVQKTIPVGQRPRGIVLSKDNRTLYICASDDDEIEVLDLDDFTLKPPLPS
                     GADPETFALHPDGRHLYVSNENNSLVSIIDIAQRAIVGEIPVGVEPEGIGISPDGKWV
                     VNTSETTNMAHFIDSDTHKIVANVLVDPRPRRAVWTADGKEVWVSSEIGGTVSVIDAT
                     SHAIAHKIAFAVPGVPSEAIQAVGIALAKDGKLGFVALGPANRVAVVDAQSFEVKKYL
                     LVGQRVWNLAFNHDETRLFTTNGISNDISEIDVPGLR"
BASE COUNT          795 a         1576 c         1560 g          637 t
ORIGIN      
        1 agaagctggc gcagccgccc ggcgctgtcc tcgtcgagcg agacgaaggg ctcgtccatc
       61 agcagcagat cgggatcgac cgcgaaggcg cgggcaatgg cggcgcgccg gctcatgccg
      121 cccgagaggc ggcccggata gacgtcgcgc gcggcctcaa gctcgaccgc gcgcagcagg
      181 cggtcgacga tgcccggatc ctggcccggc cgcagcacca gcgtgagatt ctcgcgcacg
      241 gtgcgccagg gcaggagccg cggattctgg aagacatagc cgatgcgcgg ctcgcgtccg
      301 ttctcggccg gcaacagcgc gagttcgccc gtgaaatcgc cgtcgagccc ggcgacgatg
      361 ttgaggaagg tcgtcttgcc gcacccggag ggaccgacca gcgccacgaa ctcgccggcg
      421 gggacgttca cgacgaactc cctgagcgcc agatgcgccg gcgcgccgcc gatcccggga
      481 tagcgcttct cggcgatccg cgcgcgaagg atcggcgtca gcgtcgccat cggctgctgc
      541 gctgctcgag cggctgaagg atgccccatt cgatcgcttg aaccacggcc gtgaacgcga
      601 cggtataagc caggatctcg ggcacgtcga attgctggaa gtggagctgg atggcgaagc
      661 ccacgccgtc gcttcggccc aggagttcgg cgacgagaac gatcttccag acgagagcga
      721 ggccggagcg cgcggcggcg aggaggaacg ggtagagctg cggcaggacg aagtggcgca
      781 gattgctgag gcggccgatg cgaaagctgc gcgcgacgtc ggccagatcg cggtcgagcg
      841 cgcgggcgcc ctcgcgcaag gtgacggcga cggtcgggat cttgttgacg gcgacggcgc
      901 cgatcgccgc cgcctcggtc aggccgaacc agacataagc caggacgatg acaacgaggg
      961 ccggcagatt cagcagcagc acgagccagc tgtcgaagaa cgcatcgagc cgtcgcgacc
     1021 gccccatgac aacgccgata acgcttccga tcaccatcgc gataaaaaac gcggcggcga
     1081 cgcgcagcag ggtcacgccg agatgatgcg gcagcggacc gcttaccact tccttcgcca
     1141 aggcccggaa gacggcgccc accgtcggca caagacggct ctgcgcaaag agcgcgaccg
     1201 cctcccagag cgccagcagc aagacgagag acagggtgcg gcgcaggatc gagccgccga
     1261 gctgcggcag cttcatccga cgcatgcggc gtcagtatcg aacatcgtcg aggaaagtcc
     1321 cgggctgcag cagcgggacc gggcccgcca gcgcttcgcc gccgatcttc gccagaagca
     1381 catagagccg ctcggcatcg cgccgctccg catcgcccca atggcgggga atgccggcgc
     1441 gatatgcatc gcgcagcttc acgagctcgg cggcgtcatg ggcgccggtg cgcggagcga
     1501 tccgcgtcca ttcctcgtcg gagccggcca gcagctcctc ggcgcggcgg cttgcgcgca
     1561 cgaaagcggc aaggagcggc cggtgcgccg cggcccagtg ctcggcaacg acatagccga
     1621 ccatgggcac ggcgctctcg aatcccagcg catgcatggc gtcgccgacc gacatgatct
     1681 gccgcatgcc gcgcgcctcg agccgtgccg cgaagggcca gtaggtgaga accgcatcga
     1741 gtcgtccggc cgccagctcc gcgtcgagca gcggcggcgc cgcgaaggat ttgtcggtat
     1801 cgcgctcgag atcgaggccg ctctgctgcg ccgccgccgc gcgcatgatg agccagctct
     1861 tgtcgacggc gctgccggcg atcccgagcc ggcgcccttt caggtcggca agcgtgcgca
     1921 ccggcgattg cggcgccgcc acgagcgcgc cgactgcggt cgagaaggga aagaaggtcc
     1981 agtcggcacc gctggcgcgc tgccgcgaca cccagaacca gtcgctgacg acggtgtcga
     2041 cgctgcccgc ctgcagcgcc accagcgtcg ccggcgtgct tgccagctcg acgggctcga
     2101 cggcgaagcc ttccgcggtg tcgaggccgt gctggcggat aacatcgagc tcccaactca
     2161 cggtgccgaa tttgaggacg ccgacgcgca agggatcggc ggcccgcgca ttccccgcga
     2221 gcgcgaggac ggcgaggacc aatgccgcgc ccgccgcggc aatcctccgc atggcttcct
     2281 ccctttctcg agcgcttgcc gcgccggtgg cgccgatgtt gcgtcggcag cgcccgcgac
     2341 acaagcccgc ccgcgatttg cactggcagc accggcgctt tgccgccaga atgccgccaa
     2401 acctcggcac tccgccgccg gagaggaaac gttgctcggc ccgtacacca cccgcgcgct
     2461 cctggcgctg agcctgtttg cgacgacgct gcccggcagc gccgccggag cggccgatga
     2521 agcggcaccc gccgcgctgc ggatcggatt tctctctgtg gcggtgaagc agccggcgcc
     2581 cgagccttat ctcgatccgc cgcccgcgga tgaaggcgtc gccggcgccc ggctcgggat
     2641 cgacgacgac aacaccacgg gtcgcttcac cgggcagcaa ttcgccctcg aggaggagat
     2701 ggtgccggcc gagggcgacg tcgccgccgg attccgccgc ctcgtcggca gcggcattcg
     2761 tctggtcgtg actgatctgc cgaaggcggc gctgctcgcc atcgccgatt tgccggacgc
     2821 gaaggacgtc ctgctgctcg atgcggggac ggaggatgac gagctccgcg cagagaagtg
     2881 ccgcgctaat gtgctgcaca cgctgccgag ccgcgccatg ctggcggacg ggctcgtcca
     2941 gtatctcgtg gcgaagcagt ggcggcgcgt gctcctggtc gtcggcccga ccgaggcgga
     3001 cggcgcctat gccgccgcag tcaaacgcgc gatcacgaag ttccaggcga agctggtcga
     3061 cgagaagccc tggacctatc agcccggcgc gcggcgctcc gatacgggcc attactcgat
     3121 tgccgccgag gtcgcgcgct tcacgcaagg catcagctat gacgtgctcg tcgtcgccga
     3181 cgaggatgac gaattcggcg actatctgag ctatgccacg accgacccgc ggcccgtcgc
     3241 cgggacccac ggccttgtcg ccggcgcctg ggcgcgaccg catcaggaat ggggcgcgac
     3301 gcagctccag gaccgattcc tccaccgcgc caagcgctgg atgacggatc gcgattatgc
     3361 cgcctggctg gcggtgcgcg cagcgggaga gggagcgacg cgcgcacagt cggtcgagcc
     3421 ggcgaagctc acgacgtaca tgcgcagcga caagttcgag cttgggggat acaagggact
     3481 gccgctcagc ttccgccctt gggacgggca gatgcgccag gcgatcctgc tctatgattc
     3541 gcgcgcgctc gtctcggtgt cgccgcaacc gggcttcctg caccagttct cgacgctcga
     3601 cacgctcggc ctcgaccgcc ccgagtcaaa atgccatctg ccgtgagcaa gcaccatcga
     3661 tctcgcgcga tcgctgcgct ggcactggcg tcggcgctgt ggccggcggg tccgctccgc
     3721 gcagagtcgg tgctcgtctc gaacgagaaa ggaaattcca tcaccgtcct cgatgccgag
     3781 acgctgacgg tacagaagac catcccggtc gggcagaggc cgcgcggcat cgtcctctcc
     3841 aaggacaatc gcacactcta catctgcgct agcgacgacg acgagatcga ggtgctcgac
     3901 ctcgacgact tcacgctgaa gccgccgctg ccgagcggcg ccgatcccga gacctttgcg
     3961 ctccatccgg acgggcgcca tctctatgtc tcgaacgaga acaacagcct ggtctcgatc
     4021 atcgacatcg cgcagcgtgc gatcgtcggc gagattccag tgggggtcga acccgaaggc
     4081 atcggcatca gcccggacgg gaaatgggtg gtgaacacct ccgagaccac caacatggcg
     4141 catttcatcg atagcgacac gcataagatc gtcgccaacg tgctggtcga cccgcggccg
     4201 cgccgcgcgg tctggaccgc ggacggcaag gaggtctggg tctcctccga aatcggcggc
     4261 accgtcagcg tcatcgatgc gacgtcgcat gcgatcgcgc acaagatcgc tttcgccgtg
     4321 ccgggcgtcc cgagcgaagc gatccaggcc gtcggcatcg cgctcgccaa ggacgggaag
     4381 ctcggcttcg tcgccctcgg tccggccaac cgcgtcgccg tcgtggacgc tcagagcttc
     4441 gaggtgaaga agtatctgct ggtggggcag cgcgtgtgga atctcgcctt caatcacgac
     4501 gagacgcggc tcttcaccac gaacggcatc agcaacgaca tctccgagat cgacgtgccg
     4561 ggcttgcg
//
LOCUS       DASYNS010000258         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2089798, whole genome shotgun sequence.
ACCESSION   DASYNS010000258 DASYNS010000000
VERSION     DASYNS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2089798"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VGP50_08085"
     CDS             complement(<1..317)
                     /locus_tag="VGP50_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8027376.1"
                     /translation="MDLVAQLFVDGVINGSHYALVGLGFGLIFGPTRITHFAFGPLYA
                     LAAYACWTAAVPLNLPLPVAAAVGVAAGAVSGVVTYRVLYRPFERRQSASLVVLIASL
                     GLF"
     gene            complement(473..1627)
                     /locus_tag="VGP50_08090"
     CDS             complement(473..1627)
                     /locus_tag="VGP50_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027377.1"
                     /translation="MRTLVAALAALALLAFGESRAAEPPKDPPVFAIGAILAMTGQAS
                     SIGQTMSQGMLQAVDEINAKGGIDGVKLEADIEDHKGGGGQDGVAAMNRLISIRHVQA
                     LLTSFSAPTIAVAPIADQQHVLMLNGGAVSAKLVGLTPYLFNTRSLATDLSRIAIAYA
                     HEIGAKKLAQLQWQNDVGDNVVKLADPLWTSLGGNVVATEAVAQGTTSMDTQIAKIRA
                     ANPDVIALWMFTPEAGLALKRIRDFGLKQPIIGIEYTENDATIAGPAADGYHFANDYF
                     LPSDDNPWSKQFAADYKKRYGADPDFYAANYYENTYVIAECIRRARAKGGDYWNGAKL
                     RDALLANPRFASVYGGDMVFQPNGVALKRTALFTVEGGKRKFVKFFETTQ"
BASE COUNT          277 a          524 c          618 g          287 t
ORIGIN      
        1 atgaagaggc cgagcgaggc gatgagcacg acgaggctgg cgctctgccg gcgctcgaag
       61 ggacgataga ggacgcgata ggtgacgacg cccgagaccg cgccggcggc gacgccgacc
      121 gccgccgcga ccggcagcgg caggttgagc gggaccgcgg cggtccagca ggcataagcc
      181 gcgagcgcat agagcggccc gaaggcgaaa tgcgtgatgc gcgtcggccc gaagatgagg
      241 ccgaagccga ggccgacaag cgcgtagtgg ctgccgttga tgacgccgtc gacgaagagc
      301 tgagcgacga gatccatggg tcagcgctcg ctgtcgagga gcgatgcggc ctcacccccc
      361 accctagcgc ccccctcaag gggggagggg cgtgttgacg tggcgcgata ttccccctcc
      421 ccccttgagg gggagggccg gggtgggggg tgacgcagca cggacggcgc agttactgcg
      481 tcgtctcgaa gaacttcacg aatttgcgct taccgccttc gacggtgaag agcgcggtgc
      541 gcttcagcgc gacgccgttg ggctggaaca ccatgtcgcc gccatagacg ctcgcgaaac
      601 gcggattggc gagcagcgcg tcgcgcagct tcgcgccgtt ccagtagtcg ccgcctttgg
      661 cgcgcgcgcg cctgatgcat tcggcgatga cgtaggtatt ctcatagtag ttcgccgcat
      721 agaaatcggg atcggcgccg tagcgtttct tgtagtcggc ggcgaattgc ttcgaccagg
      781 gattgtcatc cgacggcagg aagtagtcgt tggcgaagtg atagccgtcg gccgccggcc
      841 cggcgatggt cgcgtcgttc tcggtgtatt cgatgccgat gatcggctgc ttcagcccga
      901 aatcgcggat gcgcttcaag gcgagtcccg cctcgggcgt gaacatccag agcgcgatca
      961 cgtcgggatt ggcggcgcgg atcttggcga tctgcgtgtc catgctggtc gtgccctgcg
     1021 ccaccgcctc ggtggcgacg acgttgccgc cgagcgaggt ccagagcgga tcggcgagct
     1081 tgacgacgtt gtcgcccacg tcgttctgcc attggagctg cgccagcttc ttggcgccga
     1141 tctcatgggc gtaggcaatg gcgatgcggc tcagatccgt ggcgagcgag cgcgtgttga
     1201 agaggtaggg ggtgagcccg accagcttgg cgctgaccgc gccgccattc agcatcagca
     1261 catgctgctg atcggcgatg ggggcaacgg cgatggtggg cgccgagaag gaggtgagga
     1321 gcgcctgcac atggcggatc gagatgaggc ggttcatcgc ggcgacgccg tcctggccgc
     1381 cgccgccctt gtggtcctcg atgtcggcct cgagcttgac gccgtcgatt ccgcccttgg
     1441 cgttgatctc gtccacggcc tgcagcatgc cctggctcat cgtctggccg atcgagctcg
     1501 cttgtccggt catggcgagg atcgcgccga tggcgaagac cggcggatcc ttgggcggct
     1561 ccgctgcacg gctctcgccg aaagccagca aggcgagcgc ggcaagcgcc gcgacgagcg
     1621 ttctcatgat ctgcctcccc cgggtcggtc cttctttgcc gaccagattg gcagcgccgc
     1681 ggcgagggag tcaaacggcg cgagat
//
LOCUS       DASYNS010000259        10919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2558258, whole genome shotgun sequence.
ACCESSION   DASYNS010000259 DASYNS010000000
VERSION     DASYNS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10919
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2558258"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VGP50_08095"
     CDS             complement(<1..503)
                     /locus_tag="VGP50_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027378.1"
                     /translation="MAAGLDAIYSFGVKTDGRARIAMKTLLAVVGDGTAVPVLETALL
                     VARRFGSRIVGLNALTTEYAVVFGGEMGFSISSEVDRTLEREGHDRREEARSLFASFM
                     REHGVPLGAVAGEGPSAEWREEAGRQNAVVGAFGRVFDLIVVERPAKLASLAEATLED
                     ALFESGRP"
     gene            587..1111
                     /locus_tag="VGP50_08100"
     CDS             587..1111
                     /locus_tag="VGP50_08100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8027379.1"
                     /translation="MSLRIEVRPGDDSWRDIQPLFALVYPPEILAGLVWRDVTWANAD
                     SRVLVYEGERLVSAVGLYRREGRHDGMPVRLGGVGGVMTLPKHRRHGFASAGMHHARR
                     LFAEARIDFGLLFCEPKNIAFYGRRGWSVFPEPVVVEQPARRGPFTLMTAMVLGLARA
                     TPTAGMIDLCGLPW"
     gene            1179..2879
                     /locus_tag="VGP50_08105"
     CDS             1179..2879
                     /locus_tag="VGP50_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV8027380.1"
                     /translation="MTDATGLARGLTNYGDRDFALYLRRSFARSMGYSTALLERPVIG
                     IANTASGFNNCHRLVPELVEAVKRGVLAAGALPLEFPTISLGEVFLHPTSLMFRNLMA
                     MDTEEMIRAQPMDAVVLVGGCDKTVPAQLMAAVSANLPAIQLVTGPMMTGRFRGERLG
                     ACTDCRRFWARFRAGEIDRAKIDEVEGGLATTAGTCAVMGTASTMAALAEALGMMLPG
                     TAAIPAVHADRLRAAEATGAVAVALARAPIAPDRVITEKSVENARRVLLAIGGSTNAV
                     LHLTAIAGRAGIDVSLETLNTLSDTTPLLVDLKPTGPYYMEDLFAAGGIGAVLRELRP
                     LLHLDCVTVTGETLGERLAADGAHYVDRTVIRTLGAPLQPQGGLVALFGSLAPRGALL
                     KRAAADPRLFEQTGRAVVFTSLEDVARRIDDPELAVTPDDFLVLQNAGPASAAAMPEA
                     GYLPIPAKLARAGVKDMVRISDARMSGTAYGTVVLHVTPDAASGGPLARVRTGDSIRL
                     SIRERRLDLLVDPAELSARQVSIAAAPARGYARLYHREILQADRGCDFDFLRRTPLQR
                     "
     gene            complement(2947..3579)
                     /gene="bluB"
                     /locus_tag="VGP50_08110"
     CDS             complement(2947..3579)
                     /gene="bluB"
                     /locus_tag="VGP50_08110"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HEV8027381.1"
                     /translation="MTAFDAAFREQLCELIRWRRDVRHFRRDPLPSGTLEHLLELASL
                     APSVGLSQPWRFVLVDDAARRAAVRDNFRRANAEALGAYAGEKAERYAKLKLAGLEEA
                     PLQLAVFADRGTEQGHGLGRRTMPEMAEYSAVMAIHTLWLVARAEGLGLGWVSILEPA
                     EMSRILETPPVWIFLGYFCLGYPQRADVRPELEREGWERRRGWESSLLRR"
     gene            complement(3576..4721)
                     /locus_tag="VGP50_08115"
     CDS             complement(3576..4721)
                     /locus_tag="VGP50_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEV8027382.1"
                     /translation="MAATLGPNAGLVGKPGSRFALDTPALLLERDALDRNIAVMAAHA
                     LAAGVALRPHAKTHKSLRIAERQIAAGALGICCATLGEAEVMVAGGVPGVHITSPQVT
                     PAKIARLAALNAASHRGLSVVVDHPANLAALDLAAQQAERPIDVLVDFSAGQGRTGCA
                     DEADLTALVRAAVAASGVAFRGIQSYSGNLQHIPDRAERRAKALAQLERLRGIVRRLG
                     EAGIKVPIVSGAGTGTFDIDPESRVLTELQVGSYVFMDVQYHQALADGRNAPPFETAL
                     FVATSVVSINATGYVTTDGGLKCFATDGPLPEVASGAPPGSRYEFFGDEHGKLVLPTG
                     ALRPALGATIECVTPHCDPTVNLHDFYHVVRGDTLVDIWPVEARGKR"
     gene            4973..5989
                     /locus_tag="VGP50_08120"
     CDS             4973..5989
                     /locus_tag="VGP50_08120"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEV8027383.1"
                     /translation="MGRAAALGLALTMFLAGRAFAADQPIELKLSTWLPPAHPLNPSL
                     QAWATDVEKASGGTIKSTLFTSEQLGKAFDHYDMARDGIADFTYVNPGYQPGRFPVAA
                     AAELPFLMANAKGGSAAFDAWYRKYAAQEMKDVHYCMGFVLDPGTFHARKKIMRPEDI
                     SGVKVRPAGATMAAFVTLLGGANVQASAPEARDALERGVADAITFPWGSLVLFGIDKV
                     VKYHMDAALYVSDFVWVMNKTKYETLSSAQRDVIDAHCNTEWAEKIASPWADFEAAGR
                     EKVRNEPGHEVYKISPDDLAAWRKAAEPLTAKWADGVRKAGVDPDKAMAELRATLEQH
                     QAMY"
     gene            6116..6607
                     /locus_tag="VGP50_08125"
     CDS             6116..6607
                     /locus_tag="VGP50_08125"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEV8027384.1"
                     /translation="MDRFIDGIELLAAIFVGLVAADIFLSVLLRKLFTISIPDGYDFG
                     RFLLAILIFWGIAATSYRGTHITVDLVWSAAGPRVKRAIDVFATLVLLFVVTVHTAML
                     FDKVRTTRADHVLTFDLNLPVWPFFAVAWLGDVSAVLLIAVRTFRLITRPQPFPASVE
                     PVE"
     gene            6615..7916
                     /locus_tag="VGP50_08130"
     CDS             6615..7916
                     /locus_tag="VGP50_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEV8027385.1"
                     /translation="MSTPAVAVTGFIALFALMLLRVPVGMAMGLVAVTGYGYLNGAVP
                     ALKMVGQTSMRTVTEYTFGIIPMFLLMGAIVAASGMSRELFRAGNTMVGHFKGGLGIA
                     TIAACAGFAAISGSSVATAATFSTVAYPEMRQYRYPQSFATGVIAAGGTLGAMFPPST
                     VLAVYGVITQQDIGKLFIAGILPGLLAVSMYIGTIAVIGLVRPGYLPEGPRSSWRERI
                     AATLDVWAPLLLFVIVIGGLYGGWFTPTEAGGMGAGGAFLISTARRRLSPAQLLQSLL
                     HATRTAAAIFTVLIGAILFGYFLTITQTPQQVTAFLTGLGLGRYGVLALIMVMYLVLG
                     CLMDALAMVILTVPIVFPVVTALGFDPIWFGVIIVMTVELGLIHPPVGMNVFVIKTVI
                     PEVSFLTIFAGVLPFILTDILRLVILISFPVIATYLPDHMR"
     gene            7927..10062
                     /locus_tag="VGP50_08135"
     CDS             7927..10062
                     /locus_tag="VGP50_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HEV8027386.1"
                     /translation="MERSFKKEVEALRLGAGATFRGEGILAVTKALLQSGVSYVGGYQ
                     GAPVSHLLDVLIEAEDIIGELGVHLETCTNEAAAAAMLGASINYPLRGVVTWKSIVGT
                     NVASDALSNLASPGVMGGALIIVGEDYGEGASVIQERTHAFAMKSSIWLLDPRPDLPA
                     IVHLVEKAFELSEASHAPVMMELRIRACHVTGAFTAKDNVAPAHSRLKPIAGPPRFSY
                     ERLAHPPATFAQERLKVEERLPAAQAFIRTQRLNEFFAGDLADIGVIVQGGLYNSMLR
                     ALERVGLADIFGASRVPLYVLNVVYPLVSEELTQFCTGKRAVLVVEEGHPDYIEQQIN
                     VLLRRHDLQTRILGKGCLPATGEYTAEALLSGLAAFLEQAGPEGLALAPIVDTVGRVT
                     VARRAAAAALGDLPPRPPNFCTGCPERPVFSALKLMQREIGPTHISADIGCHSFATFA
                     PFSMGNSILGYGMSLASAAGVAPNLARRPIAVMGDGGFWHNGVITGVASNLFNGGDGV
                     LLIMQNGYTSATGQQFMPSSTTNRRGTAPGMSIEATLKAMGVRWLRTVRSYSVSVMLA
                     TLKDAMRSAERGLKVIIADGECQLARQRRLRVEERQKLERGERLIKTRYGVDDEICTG
                     DHSCIRLSGCPSLTLKPNPDPLRSDPVASVIESCVGCGLCGEVAHAAVLCPSFYRAEL
                     IRNPNGWDRVLHRLRRGIIKRIAGTATAA"
     gene            10059..>10919
                     /locus_tag="VGP50_08140"
     CDS             10059..>10919
                     /locus_tag="VGP50_08140"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HEV8027387.1"
                     /translation="MSAAPRPLTLLIAALGGEGGGVLTNWIVTAADALGFPVQSTSIP
                     GVAQRTGATTYYIEMLPLPRDALGGREPVLSLVPGAGDIDVVVASELLEAGRTIAGGF
                     VTPDRTTLIASTHRIFAMTERTAMGDGRLDIGRLLRAAEEQAKSRVLFDMEAAAQDSE
                     SAINAVMLGALAGAGCLPIPAEAFAAAIRAEGKSVEVNLRGFSAGLAAARRGRGAEAL
                     PSLKRTKPPTPTLASLESAADAMPKVARDIIVEGLRRLVAYQDLRYAQLYLDRLAPIR
                     DADIRTAAGGR"
BASE COUNT         1724 a         3766 c         3534 g         1895 t
ORIGIN      
        1 ggtctcccgc tctcgaacag cgcgtcctcg agggttgctt ccgcgagcga ggcgagcttg
       61 gcgggccgct cgacgacgat caggtcgaag acgcggccga acgcgcccac cacggcattc
      121 tgccgccctg cctcctcgcg ccattcggcg cttggtcctt cgcccgccac cgcgcccagg
      181 gggacaccgt gctcgcgcat gaatgacgcg aagagcgacc gggcctcctc gcgccgatcg
      241 tgaccctcgc gctcgagggt gcggtccacc tccgaagaga tggagaagcc catctcgccc
      301 ccgaatacaa ccgcgtattc ggtggtgagc gcgttcaggc cgacaatccg gctgccgaag
      361 cgccgtgcca cgagcagcgc cgtttcgagg accggcaccg ccgtcccgtc gcccaccacc
      421 gccaacaggg tcttcatggc gatcctcgct cgcccgtcgg ttttcactcc aaaggagtat
      481 atcgcgtcca gcccggcggc caagcggcag cgctggcgtt gacacagccc gcactgcggc
      541 ggttagttta ccgacccgaa gcgtgcgtcg cccgatggac gggacaatga gtctgcgaat
      601 cgaagtgcgg cctggtgacg acagctggcg cgacatccag ccgctctttg cgctggtcta
      661 tccccccgag atccttgccg ggctggtctg gcgcgacgtt acttgggcca atgcggattc
      721 gcgggtgctc gtatacgaag gtgagcgact ggtttccgcc gtagggcttt atcgacggga
      781 gggccggcat gacggcatgc ccgtccgtct tggtggcgtc gggggagtga tgacgcttcc
      841 caagcaccgc cgtcacggct tcgccagcgc cgggatgcac cacgcccggc gtctcttcgc
      901 cgaagcgaga atcgatttcg gccttctctt ctgtgagccg aagaatatcg ctttctatgg
      961 ccggcgcggt tggagcgtct ttcccgagcc cgtcgtcgtc gagcagcccg cccgtcgcgg
     1021 gccgttcacg ctcatgacgg cgatggttct ggggcttgca cgcgcaacgc cgactgccgg
     1081 catgatcgat ctctgcggcc tgccatggta gcgccgcgcg ctcggcacgg cgcctttcgg
     1141 cccgatatgt cgacacgaca gcgtcctcgg agcttgccat gaccgacgcg accggcctcg
     1201 cccgcggcct caccaactac ggggaccgcg attttgcgct ttatctgcgc cgttccttcg
     1261 cgcgctcgat gggctattcc acagcattgc tggagcgtcc ggtcatcggc atcgccaaca
     1321 ccgcgagcgg cttcaacaat tgccatcgcc tcgtgcccga gctggtcgag gccgtgaagc
     1381 gcggcgtgct ggccgccggt gccttgccgc tcgagttccc cactatttcc ctgggcgagg
     1441 tcttcctcca cccgacgagc ctgatgttcc gcaacctcat ggcgatggac acggaggaga
     1501 tgatccgagc ccagcccatg gatgcggtcg tgctggtcgg cggctgcgac aagacggtgc
     1561 cggcgcagct catggccgcg gtctcggcga atctgccggc gatccagctc gtcacggggc
     1621 cgatgatgac ggggcgcttt cgcggcgagc ggctgggcgc ctgcacggat tgccggcgct
     1681 tctgggcacg ctttcgcgcc ggcgagatcg accgcgccaa gatcgacgag gtcgagggcg
     1741 ggctcgccac cacggcagga acctgcgccg tcatgggaac cgccagcacc atggccgcgc
     1801 tggccgaggc gctcggcatg atgctgccgg gcacggcggc gattcccgcg gtccatgccg
     1861 accgcctgcg cgccgccgag gcgaccggcg ccgtcgcggt ggcgctcgcg cgcgcgccga
     1921 tcgcgcccga tcgcgtcatc accgaaaaat ccgtcgagaa tgcgcgccgc gtgctcctcg
     1981 ccatcggcgg ctcgaccaat gccgtgctgc atctcacggc aatcgccggc cgtgcgggca
     2041 tcgatgtctc gctcgagacg ctcaataccc tcagcgacac gacgccgctg ctcgtcgatc
     2101 tgaagccgac gggtccgtac tacatggagg acctcttcgc ggcgggcggc attggcgcag
     2161 tgctgcgcga gctgcgtccg cttcttcatc tcgattgcgt taccgtcacc ggcgagacgc
     2221 ttggcgaacg cttggcggcg gacggcgccc actacgtcga ccgcaccgtc attcgcacgc
     2281 tcggcgcgcc gctgcagccg caaggcggcc tggtcgcgct cttcggctcg ctcgcaccgc
     2341 gcggcgccct gctcaagcgc gccgcggccg atccgcgtct tttcgagcag acgggacgtg
     2401 ccgtcgtctt cacctcgctc gaagatgtcg cgcggcgcat cgacgatccg gaactcgcgg
     2461 taacgccgga cgatttcctg gttttgcaga acgccggccc cgcgagcgcc gcggccatgc
     2521 ccgaggcagg ctacttgccg ataccggcga agctcgcgcg cgccggcgtc aaggacatgg
     2581 ttcgcatctc cgatgcgcgc atgagcggca cggcctatgg cacggtggtg ctgcatgtca
     2641 cgcccgatgc ggcaagcggc ggtcccttgg cgcgcgtgcg aacgggcgat tccatccgtc
     2701 tcagcattcg tgagcggcgt ctcgatctgc tggtcgatcc ggcagaactt tcggcgcgac
     2761 aggtgtccat cgccgcagca cccgcgcgcg gctatgcgcg gctctatcac cgcgagatcc
     2821 tccaggcgga tcgtggctgc gacttcgatt tcctgcgacg cacgccgctg cagcgatgat
     2881 cctctgcgaa atcttatgtc accggtgatc aggaacgctg cccggatgtc acggatctaa
     2941 tagtcctcaa cggcgcagta gggagctctc ccaacctcgg cggcgttccc agccctcgcg
     3001 ctcgagctct ggcctcacgt cggcgcgttg cggatagccc aggcagaaat agccgaggaa
     3061 aatccacact ggcggcgttt cgaggatgcg tgacatttcg gccggctcga ggatcgacac
     3121 ccagccgagg cccaggcctt cggcacgggc gaccagccaa agcgtgtgga tcgccatgac
     3181 cgcggaatac tccgccatct ccggcatggt gcggcggccg agcccatggc cttgctcagt
     3241 gccgcggtcg gcgaagaccg cgagctgcag cggcgcctcc tcgagaccgg cgagcttgag
     3301 cttggcatag cgctccgcct tctccccggc ataggcgccg agcgcctccg cattggcgcg
     3361 gcggaaattg tcgcgcacgg ccgcgcgccg tgccgcatcg tcgacgagca cgaagcgcca
     3421 gggttggctc aagcccaccg atggcgcaag cgacgcgagc tcgagaagat gctcgagcgt
     3481 gccggacggc agcggatcgc ggcggaaatg gcggacatcg cgccgccatc ggatgagctc
     3541 gcagagctgt tcgcggaagg cggcatcgaa cgcggtcatc gcttgccgcg cgcctcgacc
     3601 ggccagatgt cgacgagcgt gtcgccgcgg acgacgtgat aaaaatcgtg aaggttcacc
     3661 gtcggatcgc agtggggggt cacgcattcg atggtggcgc cgagcgccgg ccgcagcgcg
     3721 ccggtcggca ggacgagctt gccatgctca tcgccgaaga actcgtagcg gctgcccggc
     3781 ggcgcgcccg atgccacttc cggcagcggc ccgtccgtgg cgaagcattt gagaccgccg
     3841 tcggtggtga cataacccgt agcattgatg ctcacgaccg acgtcgccac gaagagcgcc
     3901 gtctcgaaag gcggggcatt gcggccgtcg gcgagcgctt ggtggtactg gacgtccatg
     3961 aagacatacg agccgacttg cagctccgtg agcacccgcg actcgggatc gatgtcgaaa
     4021 gtgccggtcc cggcgccgct cacgatcggc actttgatgc cggcctcgcc gagacgccgg
     4081 acgatgccgc ggagacgctc gagctgcgcc aatgccttgg cacggcgctc ggcacggtct
     4141 ggaatatgct gcagattgcc cgagtaggac tggatgccgc ggaaggccac ccccgaagcg
     4201 gcgactgcgg cgcgcaccag cgccgtgagg tcggcctcgt cggcacagcc ggtgcgtccc
     4261 tgcccggccg aaaagtcgac gagcacgtca atgggcctct cggcctgctg cgccgcaagg
     4321 tcgagagcgg ccagattcgc gggatggtcg actacgacgc tcaagccccg atgcgaggcc
     4381 gcgttgagcg cggcaaggcg ggcgatcttt gccggcgtca cctgcggcga agtgatgtgc
     4441 acgccgggca caccgcccgc caccatcact tccgcctcgc ccagcgtcgc gcagcagatg
     4501 ccgagcgcgc cggccgcgat ttgccgctcg gcgatgcgca gcgacttgtg cgtcttggca
     4561 tgcggccgga gcgccacgcc tgcggccagc gcatgcgccg ccatcaccgc gatgttgcgg
     4621 tcgagcgcgt cgcgctcgag aaggagcgcg ggcgtatcga gcgcgaagcg gctgccgggc
     4681 ttgccgacga gccccgcatt gggaccgagg gtcgctgcca ttgccgatat catcggcggc
     4741 aaaccgccgc ctgtcgagcc ggtaacgcgg aaaccctccc tcaggagaga gccggagtga
     4801 caggtattgg ggggagtgag gagcgcagag aacggaccgg atgagcaagg gtgcgagcac
     4861 cgacggagca gcggcgcggt cggtcattgc cctggcgcca tgactgactc taacattcgt
     4921 ttgcaggcca aacagttggt gcggcaacca ccgcagcaaa gggaggggag ccatggggcg
     4981 ggcggcggca ctgggtctcg cattgacgat gttcttggcc ggccgcgcgt ttgccgccga
     5041 tcagccgatc gagctcaagc tctccacctg gctcccgccg gcgcatccgc tcaatccgtc
     5101 gctccaggcc tgggcgaccg atgtcgagaa ggcatccggc ggcacgatca agtcgacgct
     5161 cttcacctcc gagcagctgg gcaaggcatt cgatcactat gacatggcgc gcgacggcat
     5221 cgccgatttc acctatgtca atcccggcta tcagcccggc cgcttcccgg tcgccgccgc
     5281 cgccgagttg cccttcctga tggccaatgc caaaggcggc tcggccgcct tcgatgcgtg
     5341 gtaccgcaaa tatgccgcgc aggagatgaa ggacgtccat tactgcatgg gcttcgtgct
     5401 cgaccccggt accttccatg cgcgcaagaa gatcatgcgg cccgaggaca tcagcggcgt
     5461 caaggtgcgg ccggccggcg ctaccatggc cgctttcgta acgctcctcg gcggcgccaa
     5521 tgttcaggcc tcggcgcccg aggcacgcga tgccctggag cgcggcgtcg ccgacgccat
     5581 caccttcccc tggggatcgc tggtgctctt cggcatcgac aaggtggtga agtaccacat
     5641 ggacgcggcg ctttatgtca gcgacttcgt ctgggtgatg aacaagacca aatacgaaac
     5701 cctgtcgtcc gcgcaacgcg acgtgatcga cgctcattgc aacaccgaat gggccgagaa
     5761 gatcgccagt ccgtgggccg atttcgaggc cgccggacgc gagaaggtcc gcaacgagcc
     5821 gggacacgag gtctataaga tctcccctga cgacctcgcg gcttggcgca aggcggcgga
     5881 gccgctgacc gcgaaatggg ccgacggggt gcgcaaggct ggcgtcgatc ccgacaaggc
     5941 gatggccgag ctcagggcca cgctcgagca gcaccaagcg atgtattgag cggatcgcag
     6001 gtgcgtcgct tcgcgagtgg tctccttctc ctgcctcgcg agaggaatca atggcgctga
     6061 aatcgacggc gacatgagct cgtcaggagc aaaggcggca gcgaagcgcg gtccgatgga
     6121 ccgtttcatc gacgggatcg aattgctcgc cgcgatcttc gtcggactgg tcgccgccga
     6181 cattttcctg tcggtgctgc tgcgcaagct cttcacgatc tcgatccccg atggctacga
     6241 cttcggacgg ttcctcctgg ccatcctcat cttctggggc attgccgcaa ccagctatcg
     6301 cggcacacac atcaccgttg acctcgtctg gtccgcggcc gggccccgcg tgaagcgcgc
     6361 gatcgacgtc ttcgccacgc tggttctgct gttcgtggtg accgtgcaca cggctatgct
     6421 gttcgacaag gtccgcacca cccgcgccga ccatgtgctg accttcgatc tcaacctgcc
     6481 ggtctggccg ttctttgccg tggcatggct gggcgacgtc tccgcggtgc tgctgattgc
     6541 cgtgcggacg ttccgcctga tcacccggcc gcagcctttc ccggcttcgg tggaaccggt
     6601 cgaatgagct gtcgatgagc acccctgccg tcgctgtcac cggcttcatc gccctttttg
     6661 cgctgatgct gctgcgcgtg ccggtcggca tggcgatggg attggtcgcc gtcaccggct
     6721 atggctatct caacggggcc gtgccggcgc tgaagatggt cgggcagacc tcgatgcgga
     6781 cggtgacgga atacaccttc ggcatcattc ccatgttcct gctgatggga gcgatcgtcg
     6841 ccgcctcggg catgagccgc gagctgtttc gcgccggcaa caccatggtc gggcacttca
     6901 agggcggcct cggcattgcc accatcgcgg cctgcgccgg ctttgcggcg atcagcggct
     6961 cgtcggtggc gacggcggcc accttctcga ccgtggcgta tccggagatg cgtcaatacc
     7021 gctatccgca gtccttcgcg accggcgtta tcgccgcggg cggcacgctc ggcgcgatgt
     7081 ttccgccctc gaccgtgctc gcggtctatg gcgtcatcac gcaacaggac atcggcaaac
     7141 tgttcattgc gggcatactc ccgggactgc tcgccgtctc catgtatatc ggcaccatcg
     7201 ccgtgatcgg gctggtccgt ccgggctatc tgccggaggg accacgcagc tcctggcgtg
     7261 agcgcatcgc ggcgaccctc gatgtctggg ctccgctgct gctttttgtc atcgtcatcg
     7321 gcggactcta tggcggctgg ttcacgccga ccgaggccgg cggcatgggt gcgggcggcg
     7381 cctttctcat cagcaccgcg cgccggcggc tctcgccggc ccagctgctg caatcgctgc
     7441 tccatgcgac acgcaccgcg gcggcaatct tcacggtgct gatcggcgcg attctcttcg
     7501 gctacttcct cactatcact cagacgccgc aacaggtaac ggcattcctc accggacttg
     7561 gcctcggacg ttacggcgtg ctggcgctga tcatggtgat gtatctggtc cttggatgcc
     7621 tcatggatgc gttggcgatg gtcatcctga cggtgccgat cgtctttccg gtggtgacgg
     7681 cactgggctt cgaccccatc tggttcggcg tgatcatcgt catgacggtc gagcttggcc
     7741 tcatccatcc gccggtcggc atgaatgtgt tcgtcatcaa gacggtgatc cccgaggtca
     7801 gtttcctgac gatcttcgcc ggggttctgc ccttcatcct caccgacatc ctccgcctcg
     7861 tgatcctcat cagctttccc gtcatcgcga cgtatctgcc ggaccatatg cgttaggagc
     7921 cacgccatgg aacgctcgtt caagaaggag gtggaagcgc tgagactcgg cgccggcgcg
     7981 accttccgcg gcgaaggcat tctcgcggtc accaaggcat tgctgcaatc gggcgtcagc
     8041 tatgtcggcg gatatcaggg agcgccggtg tcgcatcttc tcgacgtgct catcgaggcc
     8101 gaagacatca tcggcgaact cggcgtccac ctcgagacct gcaccaacga ggcagcggcg
     8161 gcagcgatgc tcggcgcctc gatcaactat ccgctgcgcg gcgtcgtcac atggaagtcc
     8221 atcgtcggca ccaatgtcgc ctcggacgcg ctctccaacc tcgcctcgcc cggcgtgatg
     8281 ggcggcgcgc tcatcatcgt cggcgaggat tacggcgaag gcgccagcgt catccaggag
     8341 cgtacacacg ccttcgccat gaaatcgtcg atctggttgc tcgacccgcg ccccgatctg
     8401 ccggcgatcg tgcatctggt cgagaaggcc ttcgaactgt cggaggccag ccacgccccc
     8461 gtgatgatgg agctgcgcat ccgcgcctgc cacgtgaccg gcgcgttcac cgcaaaagac
     8521 aacgtcgcgc cggcgcattc gcgcctcaag cccatcgccg gcccgccgcg cttctcctat
     8581 gagcgtctcg cccatccgcc ggccactttc gctcaggagc ggctcaaggt cgaggaacgg
     8641 ctgccggccg cccaggcctt catccgcacg cagcggctca acgaattctt tgccggcgat
     8701 ctcgccgaca tcggcgtcat cgtccaaggc ggactttaca acagcatgct gcgcgcgctg
     8761 gagcgcgtcg gtcttgccga catcttcggc gccagccgag tgccactcta tgtcctcaat
     8821 gtcgtctatc cgctggtgtc cgaagagctg acgcaattct gcaccggcaa gcgcgccgtg
     8881 ctcgtcgtcg aggaagggca ccccgactac atcgagcagc agatcaatgt cttgctgcgg
     8941 cgccacgact tgcaaacgcg cattctcggc aagggatgcc tgccggccac cggtgaatac
     9001 acggccgagg ctctcctttc cggcctcgcg gccttcctcg agcaagcggg gccagagggt
     9061 ctcgccctcg cacccatcgt cgatacggtc ggccgcgtca cggtcgcccg gcgcgccgct
     9121 gctgccgcgc ttggcgacct gccgccgcgg ccgccgaatt tctgcaccgg ttgcccagag
     9181 cgtccggtgt tcagcgcctt gaagctcatg cagcgcgaga ttggtccgac ccatatcagc
     9241 gccgacatcg gttgtcattc cttcgcgact tttgcgccct tcagcatggg caattcgatc
     9301 ctcggctatg gcatgagcct cgcgagcgcc gccggcgtcg cgcccaacct ggcgcggcgc
     9361 ccgatcgccg tgatgggcga tggcggcttc tggcacaatg gtgtcatcac cggcgtcgcc
     9421 agcaatctct tcaacggcgg cgacggcgtg ctgctgatca tgcagaacgg ctacacctcg
     9481 gccaccggcc agcaattcat gccctcgagc acgacgaacc gccgtggcac ggcgccaggc
     9541 atgagcatcg aggcgacgct caaggcgatg ggcgtacgct ggctcagaac ggtgcgcagc
     9601 tacagcgtat ccgtgatgct ggcgactctc aaagacgcca tgcgcagcgc cgagcgcggc
     9661 ttgaaagtga tcatcgccga cggcgagtgc cagctcgcgc gccagcgccg cctccgcgtc
     9721 gaagaacggc agaagctcga gcgcggcgag cgcctgatca agacgcgcta tggcgtcgac
     9781 gacgagatct gcaccggcga tcactcctgc atccgcctgt cgggctgtcc ttcgctcacc
     9841 ctcaagccca atcccgatcc gctgcgcagc gatcccgtgg caagcgtcat cgagagctgc
     9901 gtcggctgcg gtctctgcgg cgaggtcgcg catgcggcgg tgctgtgtcc gtccttctac
     9961 cgcgccgagc tcatccgcaa cccaaacggc tgggatcgcg ttctccaccg cttgcgccgc
    10021 gggatcatca agcgcatcgc cggcacggcg acggccgcat gagtgccgcg cctcgaccgc
    10081 tgacactact cattgccgcg ctgggcggcg aaggcggcgg cgtgttgacg aactggatcg
    10141 tcaccgccgc ggatgccctt ggtttcccgg tgcagagcac gtccatcccg ggtgtcgccc
    10201 agcgcacggg tgccaccacc tactacatcg agatgctgcc gctaccgcgc gacgcgctcg
    10261 gcggcaggga gcccgtgctt tccctcgtgc ccggcgcggg cgatatcgac gtggtcgtgg
    10321 cgagcgagct cctggaagcc ggccgcacga tcgccggcgg cttcgtcaca ccggatcgca
    10381 cgacgctcat cgcttcgact catcggatct ttgccatgac cgagcgcacc gccatgggcg
    10441 acggccgctt ggatatcggc cgcttgttgc gcgcggccga agagcaggcg aagagccgcg
    10501 ttcttttcga catggaagcg gccgcgcaag actccgagag cgcgatcaat gccgtgatgc
    10561 tcggcgcact cgccggcgcc ggctgcctgc cgattccggc cgaagccttc gccgcggcaa
    10621 ttcgcgccga aggcaaatcc gtcgaggtca atctccgcgg cttctcggcc gggctcgcgg
    10681 cggcgcgacg cggccgcggc gcagaagcgc tgccgtccct caagcggacg aagccgccca
    10741 cccccaccct cgcgtcgctc gagagcgccg ccgatgctat gcccaaagtc gcgcgcgaca
    10801 tcatcgtcga aggattgcgg cgcctcgtcg cgtatcagga tctgcgctat gcccagcttt
    10861 atctcgaccg cctcgcgccg atccgcgatg ccgacatccg gacggccgcc ggcggccgg
//
LOCUS       DASYNS010000260         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2558289, whole genome shotgun sequence.
ACCESSION   DASYNS010000260 DASYNS010000000
VERSION     DASYNS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2558289"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..>1692
                     /locus_tag="VGP50_08145"
     CDS             14..>1692
                     /locus_tag="VGP50_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEV8027388.1"
                     /translation="MTDNSDNSEHEQKRPLSLVRPGSRLELRKSIETSQVRQSFPHGR
                     SKTVQVEIVRKPRAAPGVIERRETPPPKDATKPTTAATQPGRRPQVIPRGLTDKEKAG
                     RVRALQGAIRADEDARRQAVIEADREREEEQRRAAEAQHRAEEEARRRTDEETKRKSE
                     DEVRRRTDEETKRKSEEEARRQMAERAGKAAADKVAALTAAGKVKVVEEETEEETPAR
                     RGARAEVKKAPATTRRDEPRRRTGKLTITRVLNEEDGERMRSLASVRRQREREKQRAL
                     QALQEPTKIVRDVVVPETITVQELANRMAERGADVIKTLMRMGVMATINQAIDADTAE
                     LIVGEFGHRIKRVSESDVEIGLVGDTDPEDQLVPRPPVVTIMGHVDHGKTSLLDALRD
                     TDVASGEAGGITQHIGAYQVQLASGQRITFIDTPGHQAFTAMRARGAQVTDIVVLVVA
                     ADDGIKEQTVEAIRHAKEAKVPMIIAINKMDKPDAKPDRVRQELLQHEIVVEKLGGDV
                     LDVEVSATKRTNLDKLLEAILLQAEILDLRANPDRPADGTVIEAKIERGRGP"
BASE COUNT          373 a          555 c          592 g          172 t
ORIGIN      
        1 gtatcgagga agaatgaccg ataacagcga caacagcgag cacgaacaga agcgtccgct
       61 cagcctcgtg cggccgggca gccggcttga gctgcgcaag tcgatcgaga ccagccaagt
      121 ccgccagagc ttccctcacg gacggtcgaa gacggtgcag gtcgagatcg tccgcaagcc
      181 ccgcgcggca ccgggcgtca tcgagcggcg ggagacgccg ccgccgaaag acgcgacgaa
      241 acccacgacc gccgcgaccc agccgggacg gcggccgcag gtgattccgc gcggcctcac
      301 cgacaaagag aaggccgggc gcgtgcgcgc gctacaaggc gcgatccgag cggatgaaga
      361 tgcgcgccgc caggcggtca tcgaggccga tcgcgagcga gaggaggaac agcgtcgcgc
      421 agccgaagcg cagcaccgcg ccgaagagga ggcgcgccgc cgcaccgacg aagaaaccaa
      481 gcgcaagagc gaagacgagg tgcgccgccg caccgacgaa gaaaccaagc gcaagagcga
      541 ggaagaggcg cgccgccaga tggcagagcg cgccggcaag gcagccgcag acaaggtcgc
      601 ggcgctgacc gccgccggca aggtcaaagt ggtcgaggag gagaccgagg aggagacgcc
      661 ggcccgtcgc ggcgcgcgcg ccgaggtcaa gaaagcgccg gcgacgacac gtcgcgacga
      721 gccgcgccgg cgcaccggca agcttaccat tacccgcgtc ctcaacgagg aggacggcga
      781 gcggatgcgg agcctcgctt cggtacgacg ccagcgcgag cgcgagaagc agcgcgcctt
      841 gcaggcgctg caggagccga ccaaaatcgt gcgcgacgtg gtcgtgcctg agacgatcac
      901 cgtacaggag ctcgccaacc gcatggcaga gcgcggggcc gacgtaatca agacgctgat
      961 gcggatgggc gtgatggcga ccatcaatca ggcgatcgac gccgacaccg ccgagctcat
     1021 cgtcggcgaa tttggacacc ggatcaagcg cgtctcggaa tcggacgtgg agatcggcct
     1081 ggtcggcgac accgaccccg aggaccagct cgtgccgcgg ccgccggtgg tcacgatcat
     1141 gggccatgtc gaccacggca agacctcgtt gctcgacgcg ctgcgcgaca ccgacgtcgc
     1201 gagcggcgaa gcaggcggca tcacccagca catcggcgcg tatcaggtgc agcttgcgag
     1261 cgggcagcgc atcaccttca tcgacacgcc gggccaccag gcctttaccg ccatgcgggc
     1321 gcgcggagcc caggtcaccg acatcgtcgt gctggtggtg gcagccgatg acggcatcaa
     1381 ggagcagacg gtcgaggcga tccggcacgc caaagaggcc aaggtgccga tgatcattgc
     1441 catcaacaag atggacaagc ccgacgccaa acccgaccgc gtgcgccagg agctgctcca
     1501 gcacgagatc gtggtcgaga agcttggcgg cgacgtgctc gacgtcgagg tatcggcaac
     1561 aaaaaggacg aatctcgaca aacttctgga agcgatcctg ctgcaggccg agatcctcga
     1621 tctcagggcc aatccggacc ggccggccga cggtaccgtc atcgaggcga agatcgagcg
     1681 cggccgcggc cc
//
LOCUS       DASYNS010000261         5068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_686484, whole genome shotgun sequence.
ACCESSION   DASYNS010000261 DASYNS010000000
VERSION     DASYNS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686484"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VGP50_08150"
     CDS             complement(<1..230)
                     /locus_tag="VGP50_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV8027389.1"
                     /translation="MAENATGAAPPQANGAVHQQLIVNVQYIKDLSFENPRAPHSLLQ
                     QKEPPEVQLSVDVKAQGLGQSVYEVLLTVSAKA"
     gene            436..1353
                     /locus_tag="VGP50_08155"
     CDS             436..1353
                     /locus_tag="VGP50_08155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEV8027390.1"
                     /translation="MERLAVLAAAIAPALVLLGYGVAKGRGSWQNHALWTAFLAGAAV
                     AIAPLAAQGWLEDTLQLGRLSPTLDAASTALLVAAIPEETAKFLVVVAIAGRWRDARR
                     IQDIIVLALGAALGFAFVENLAFVTSPGDWQTVALMRASIAVPGHGIDGLTMGALLTM
                     ARLNPERRWLFWVAALLLPIALHASYDFPLLLLEGGAAPSWVGVAWLIIIGFSAVVAI
                     SLTNRVLAVTLSRRFAAERRAEMSAAALILGGAALFLAGPLLGASLYLLSRGRLPFSG
                     AAPIILPAALGGDLLRSGLQRRRGPLALL"
     gene            1430..2080
                     /locus_tag="VGP50_08160"
     CDS             1430..2080
                     /locus_tag="VGP50_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HEV8027391.1"
                     /translation="MSDGFQYLDILLFAGIAGFLIVRLWSVLGRRTGHERPRDSMLPR
                     APIETPPAPDPVVALPPRGIAAPADAGGLARLKGADRSFDEAAFLRGARAAFEIIVNA
                     FAKGDTAALQPLLSESVYASFADAIRQRQAAHETLATNLISIKTVEIAGAEIEGETAF
                     VTVKFVSDQTNVTRAGDGSVVDGSPDHVVEKTDLWTFARAVRARDPNWTLAATHSA"
     gene            2087..3253
                     /locus_tag="VGP50_08165"
     CDS             2087..3253
                     /locus_tag="VGP50_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEV8027392.1"
                     /translation="MILLRRAAGGALAVALLLGGCAPPQPDRLTLAASRFADLPGWSE
                     DGLADAMTALRRSCAPLLAAKPEPATPLGNGNAGTAGDWLEPCAAANAVDSGDRAALH
                     AYFETWFQPWKAGNNGNSQGLFTGYYQPELRGARQPDAVYATPLLRRPPDLVTVDLHL
                     FRPALHDRIAGRVADGRLLPYESRDKIERGALDRYGLAFLWVDDPVAAFFLQIQGSGR
                     IRLPDGSMVQVGYDGQNGHPYVAIGRLLAERGLMDRDSVSLQTISAWIKAHPAEGAAL
                     MDENPSYVFFREIAGDGPLGSQGEVLTPGRSLAVDRDFIPLGVPIWLDVDNAGERLRR
                     LMVAQDTGGAIRGPVRGDVFWGFGEEAEAHAGPMKARGEYYLLLPKTVTPATAR"
     gene            3437..3865
                     /locus_tag="VGP50_08170"
     CDS             3437..3865
                     /locus_tag="VGP50_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HEV8027393.1"
                     /translation="MRKIISTLVVLCTALCACAQPAQPGEGEPATVGVNKTSGVSQVG
                     AGAGGLIGSQIGASLDRGDQAALHQTTQTALETAPANQALPWRNPNSGNYGTVIPGAV
                     YQNQGQYCREFQQTLVVGGQQQQGDGKACRQPDGSWKVVQ"
     gene            3963..4742
                     /locus_tag="VGP50_08175"
     CDS             3963..4742
                     /locus_tag="VGP50_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV8027394.1"
                     /translation="MADTPLRVGLFVTCLVDLFRPSVAFAAVKLLEAAGCVVEVPRGQ
                     TCCGQPAYNSGDRADAQAIAQGVIEAFEGFDYVVAPSGSCGGMLREHYPQLFADDPAL
                     FQRAQHLAKRSYELVSFLVDVLGLGRVAARYAGVVAYHDACSGLRELGVKQQPRTLLS
                     RVAGLTLKELPGAEICCGFGGTFCVKYPEISERMVSQKVADIAATGADTLLAGDLGCL
                     LNIAGRLTRLGSPVAVRHVAEVLADMTDALPPIGAAADGAR"
     gene            4742..>5068
                     /locus_tag="VGP50_08180"
     CDS             4742..>5068
                     /locus_tag="VGP50_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV8027395.1"
                     /translation="MQPTSHAFAENVHRALGDATLQKALQRMRTGFPGKRAQAIARLP
                     EFEALRDQAKAIKDHVLAHLDFYLETFEQKVLAQGGAVHWCRTPEEACATVLAICRRL
                     GAKTVTK"
BASE COUNT          728 a         1733 c         1702 g          905 t
ORIGIN      
        1 gctttcgccg acaccgtcag cagcacctcg taaacgcttt ggccgaggcc ttgcgccttc
       61 acgtcgacgc tgagctggac ctcgggcggc tctttctgct gcagcaggct gtgcggcgcg
      121 cgcgggttct cgaaggagag gtccttgatg tactgcacgt tgacgatgag ctgctggtgg
      181 accgcgccat ttgcctgcgg cggagcggcg ccggtcgcat tctcggccat gaagcactcc
      241 ctttggtatg atggctggct agcacggatc gcccgtcatg cacaaccgtg ctgcgcccgg
      301 cggccgccac accccgtccg gttgccgaca acctccggtt aacccttacg ctctatatgg
      361 tatatggggc aagcgctgcc caacgagagt ccgtgtgacc atctccgccg atttcaaccc
      421 caccgaggac cgctgatgga acggctcgcc gttctcgctg cggcgatcgc cccggcgctg
      481 gtgcttttgg ggtatggcgt ggccaaggga cgcggcagct ggcagaacca cgcgctttgg
      541 acggcgttcc tcgcaggcgc tgccgttgct atcgcgcccc tcgccgccca gggctggctc
      601 gaggatacgc tccagctggg ccggctgtcg ccgacgctcg atgccgcaag cacggcgctg
      661 ctggtggcgg ccatccccga ggaaacggcg aagttcctcg tcgtcgtcgc catcgccgga
      721 cggtggcgcg atgcgcggcg cattcaggac atcatcgtgc tggcgctggg tgcggccctc
      781 ggtttcgctt tcgtcgagaa cctggctttc gtcaccagcc cgggcgactg gcagacggtg
      841 gcgctgatgc gcgcctcgat cgccgtgccg gggcatggga tcgacggcct caccatgggg
      901 gcgctcctga ccatggcgcg gctcaacccg gagcggaggt ggctgttctg ggtcgcggcc
      961 ctgctgctgc cgattgcctt gcacgcctcc tacgactttc ccctcctgct gctcgaaggc
     1021 ggcgcggcgc cgtcctgggt cggcgtcgcc tggttgatca tcatcggctt ctcggccgtc
     1081 gtcgccatca gcttgaccaa tcgggtgctg gccgtgacgc tgtcgcggcg gttcgctgcc
     1141 gagcgccgcg ccgaaatgtc ggcggcagcg ctgattctgg gcggcgcggc cctctttctc
     1201 gccgggccgt tgctgggcgc gtcgctctac ctgctgagcc gcgggcgcct gccgttttcg
     1261 ggcgccgcgc cgatcatctt gccggcagcc ttgggcggcg atctcctgcg cagtggcctg
     1321 cagcgccggc gcgggcctct cgcgctcctc tgacggcgga gtacgccggc ttgccctttg
     1381 ggtgcagcgg ccctatttgt ggtagggatc ccgccaccgg actcggcaga tgagcgacgg
     1441 ttttcaatac ctcgatatcc ttctctttgc cggcattgcg ggcttcctca tcgtgcgttt
     1501 gtggagcgtg ctgggccggc gcaccggcca tgagcggccg cgggacagca tgctgccgcg
     1561 ggcgccgatc gaaaccccgc cggcgccgga tcccgtcgtt gctttgccgc cgcgcggcat
     1621 cgcagccccg gcggatgccg gcgggctggc gcgcctgaag ggcgccgatc gcagcttcga
     1681 cgaagccgcc ttcctcaggg gcgcgcgcgc tgcgttcgag atcatcgtca atgccttcgc
     1741 caagggcgac acggcggcgc tccagccgct tttgagtgaa agcgtctatg cgtccttcgc
     1801 ggacgcgatc cgccagcgcc aggcggcgca cgagaccctc gccaccaatc tcatctcgat
     1861 caagacggtg gagatcgcgg gcgccgagat cgagggcgag accgcgttcg tcaccgtcaa
     1921 attcgtctcc gaccagacca acgtcacacg cgccggcgac ggtagcgtcg tcgacggatc
     1981 gccggatcat gtggtcgaga agaccgatct ttggaccttc gcccgcgccg tccgcgcgcg
     2041 cgatcccaat tggacgctgg ccgccacgca tagcgcttag tcgcccatga tcctgcttcg
     2101 ccgggccgct ggcggcgccc tcgctgttgc tctgctgctg ggcggttgtg ctccgccgca
     2161 gcccgaccgg ctcacattgg cggcgagccg cttcgccgac ctgcccggtt ggtcggagga
     2221 tggtctcgcc gatgcgatga cggccctgcg ccgctcttgc gcgcctcttc ttgcggcgaa
     2281 gccggaaccg gcgacgccgc tcggaaatgg caatgcggga acggcggggg attggctcga
     2341 gccctgtgcc gcggccaatg ccgtggattc cggcgatcga gccgcgctgc acgcctattt
     2401 cgaaacctgg ttccagcctt ggaaggccgg caacaatggc aacagccagg gtctcttcac
     2461 gggctactac cagcccgagc tgcgcggcgc gcggcaaccg gacgcggtct acgcgacacc
     2521 gctgctgcgc cgcccgccgg atctggtgac cgtcgacctc catctcttcc gtccggcgct
     2581 gcacgatcgc atcgccggcc gcgtcgccga cggacggctg ctcccttatg agagccgcga
     2641 caagatcgag cgcggggcgc tcgaccgcta cggccttgct ttcctttggg tcgatgatcc
     2701 cgtcgccgcc ttcttcctgc agatccaggg atcggggcgg atccggctgc ccgacggctc
     2761 gatggtgcag gtcggatatg acgggcagaa cggtcacccc tatgtggcga tcggccggct
     2821 gctggcggag cgcgggctca tggaccgcga cagtgtctcg ctgcagacga tcagcgcctg
     2881 gatcaaggcg catcccgcgg agggcgccgc gctgatggac gagaacccgt cctacgtctt
     2941 tttccgcgag attgccggcg acggcccgct cggcagccag ggcgaggtgc tcacgcccgg
     3001 ccgcagcctt gccgtcgacc gcgatttcat cccgctgggc gtgccgatct ggctcgacgt
     3061 cgacaatgcc ggcgagcgcc tgcggcggct gatggtcgcg caagacaccg gcggcgccat
     3121 ccggggcccg gtgcgcggcg acgtcttctg gggcttcggc gaggaagccg aggcgcatgc
     3181 cgggccgatg aaggcgcgcg gcgaatacta tcttctgttg cccaagaccg ttacaccggc
     3241 gacggcgcgt tgatctctcg gattggcgtc gaacccggaa aatgtcgcaa gggccaatcc
     3301 cagaattgat tctttaactg gcccgttgta acgccgactt aatcccgtgt gcgtcacaac
     3361 acaggtgagc tgcggcggac agcggcgccc gatgcagcaa agctcaggcg accgcctgga
     3421 cacagagagg gcaaaaatgc gtaagattat ttccaccctg gtggtgcttt gcaccgctct
     3481 ctgtgcttgt gcgcagccgg cgcagccggg cgagggcgag ccggccacgg tcggcgtgaa
     3541 caagacgtcg ggcgtctcgc aggtcggcgc tggtgcgggc ggcctcatcg gctcgcagat
     3601 cggtgcttcg ctcgatcgcg gcgatcaggc ggcactgcat caaaccacgc agacagcgct
     3661 cgagaccgcg ccggcgaatc aggcactgcc ctggcgcaac cccaattccg gcaattacgg
     3721 cacggtgata ccaggggccg tctatcagaa tcaggggcaa tactgccgcg agttccagca
     3781 aacgctcgtc gtcggcggcc agcagcagca gggcgatggc aaagcctgcc ggcaaccgga
     3841 cggcagttgg aaggtcgttc agtaaaccgc tcgatcgtcg tctcgaagtc ctttgcgatt
     3901 gctttccacg ggccggctga ccatgctccg ggcacgtggt tgccctgaga gaagcatcgc
     3961 tgatggccga caccccgttg cgcgtgggcc tcttcgtcac ctgcctcgtc gatctgttcc
     4021 gcccctcggt cgccttcgcc gcggtcaagc tgctggaagc ggctggttgc gtcgtcgagg
     4081 tgccgcgcgg gcagacctgc tgcggccagc ccgcctataa ttccggcgat cgcgccgacg
     4141 cccaggcgat cgctcaaggc gtcatcgaag cgttcgaggg gttcgactac gtggtagcgc
     4201 cgtcgggctc ctgtggcggc atgctgcgcg aacactatcc gcagctcttc gccgacgatc
     4261 ccgccctctt ccagcgcgcc cagcatttgg cgaaacgcag ctacgagctc gtctcctttc
     4321 tcgtcgacgt gctgggcctc ggccgtgtcg ccgcgcgcta cgccggcgtt gttgcctatc
     4381 acgatgcctg ctcgggcctg cgcgagctcg gcgtcaagca gcagccgcgg acgctcttgt
     4441 ctcgcgtcgc cggcctgacc ttgaaggagc tgccgggcgc cgagatatgc tgcggcttcg
     4501 gcggcacgtt ctgcgtcaaa tatccggaga tctccgagcg gatggtctcg cagaaggtcg
     4561 ccgacatcgc ggcgaccggc gccgatacgc tgctcgccgg cgatctcggc tgtctcctca
     4621 acatagccgg acggctgacg cggctcggca gcccggtcgc cgtgcgtcac gtcgccgagg
     4681 tgctggcgga catgaccgat gcgctgccgc cgatcggcgc tgcggccgat ggggcgcgct
     4741 gatgcagccg acgagccacg ccttcgccga gaatgtgcat cgcgcgctcg gcgatgcgac
     4801 gctgcaaaag gcgttgcagc gcatgcgcac gggatttccc ggaaagcgcg cccaggcgat
     4861 cgcgcgactg cctgagttcg aggccttgcg cgaccaggcg aaagcgatca aggaccacgt
     4921 cctcgcgcat ctcgatttct atctcgagac gttcgagcag aaggtcctgg ctcagggtgg
     4981 cgccgtccat tggtgccgca cgcccgagga ggcgtgcgcg accgtgctcg cgatctgccg
     5041 ccggctcggc gccaaaacgg tcaccaaa
//
LOCUS       DASYNS010000262         6725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3182925, whole genome shotgun sequence.
ACCESSION   DASYNS010000262 DASYNS010000000
VERSION     DASYNS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3182925"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VGP50_08185"
     CDS             <1..391
                     /locus_tag="VGP50_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8027396.1"
                     /translation="PMPGKTASFGMVFPEHPQRVFAAAELSDGTLRYLALAGALLAYR
                     LPAFVALNEPETSLHPELLPLLPALGRLIARAASRTQVWVVTHSEILASAIAAHGNVA
                     PLTVIKRDGETWIEGLTLAGEFREDEE"
     gene            complement(397..729)
                     /gene="hisE"
                     /locus_tag="VGP50_08190"
     CDS             complement(397..729)
                     /gene="hisE"
                     /locus_tag="VGP50_08190"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEV8027397.1"
                     /translation="MSRSAIERLHAAVLSRGSDGLQFPRTAKLLRAGSSQIAKKLAEE
                     AVEVALDTVKNDRAAVIAESADLLYHLVVLWAALGVWPVEVWEEMERREDILGIAGKM
                     PKSSESAG"
     gene            complement(726..2447)
                     /locus_tag="VGP50_08195"
     CDS             complement(726..2447)
                     /locus_tag="VGP50_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV8027398.1"
                     /translation="MAGMILLDLIGGVALLLWGLHMVQSGVLRAFGGDLRWFLGKALS
                     NRFRGFAFGLAVTAVLQSSTATALMVSSFAAGGFIALVPALAIMLGANVGTTLLVQLL
                     SFNVMSLAPVLLAGGVLAFKRAPKTRIRDLGRVAIGLGLILLSLHILLDTLAPAEDAP
                     AVRAVLAGITGEPVLNLLAGALFAWAAHSSVAAVVVVMSLSFSHFVTPAAALALALGA
                     NLGSALNPVMEASGSNPASQRVPVGNLINRLVGCAIVLPFLGPIADALAVVDPSPVRQ
                     VADFHTAFNLVMAALFILPLRSYARLLTFLFPERKKAADPAAPLYLDPSVLSTPTLAL
                     TSAAREVLHMGDIVESMLSRTMTAFLSDDRKLVAEIERMDNVVDKLYEAVKQYVTQLT
                     RESLDEQSARRAIEIIAFSINLEHIGDIIDKNLMQLAGKKIKNRLKFSAEGSAELEAL
                     HRQVLDNLKLALGVFMSGDVAIARRLLGEKVHIREAERAAADSHLGRLREGRPESVET
                     SSLHLDILRDLKRIHSHICSVAYPVLETTGELEPSRLRHWDAQAEAAAAIPPGQEPAG
                     GIAEEPS"
     gene            complement(2607..2786)
                     /locus_tag="VGP50_08200"
     CDS             complement(2607..2786)
                     /locus_tag="VGP50_08200"
                     /inference="COORDINATES: protein motif:HMM:NF025146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEV8027399.1"
                     /translation="MYWVIGGEYTDLSFKTLVPGAREERHGPFATYKDAYDVWQSRAR
                     ATIDDAMVRFRIIEQ"
     gene            2867..4375
                     /locus_tag="VGP50_08205"
     CDS             2867..4375
                     /locus_tag="VGP50_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEV8027400.1"
                     /translation="MPYASLRDFIARLEAAERLVRVRTPVSPVLEITEIHTRLLAEKG
                     PAALFESVSRPEGGRYGMPVLTNLFGTVERVAWGMDREPHQLREIGETLAFLKQPEPP
                     GGWREALEMLPLLKTVLAMKPKTVGSAPCQDVVLTGTDIDLGRFPIQTCWPGEPAPLI
                     TWPLVVTKGPGKRREDDYNLGIYRMQVIGRDTTLMRWLKHRGGAQHHQRWSQQKREPL
                     PAAAVIGADPGTILAAVTPVPDTLSEYQFAGLLRGRKVELVDCRTIPLKVPAQAEIVL
                     EGHVSLDDDRDEGPYGDHTGYYNAVERFPVFRISAITMRRDPIYLTTFTGRPPDEPSV
                     LGEALNEVFIPLLTQQFPEIVDFWLPPEGCSYRIAVVSMKKAYAGHAKRVMMGIWSFL
                     RQFVYTKFVIVVDDDIDARDWKDVMWAVSTRMDPARDITVIDGTPIDYLDFASPEAGL
                     GGKIGLDATNKWPGETKRQWGRKIRMTDDVIAQVTAKWPTLGLPGTGKAIWK"
     gene            4701..5693
                     /locus_tag="VGP50_08210"
     CDS             4701..5693
                     /locus_tag="VGP50_08210"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEV8027401.1"
                     /translation="MARGLRSALKKHPLMMMAGALMIAATLAAAAFYAYAGAYGFNVV
                     LRRGSTIWVTVRADDPRLSPSMRRALQQPPPLATPGPFAWDDVAAGFAVGELPVLADG
                     HEVDRLLLARIDPQRFRFVLRNAPAGNRALGNWMAALHAVLVVNGSYFATDGTPATPF
                     RSDGIALGPRDYKAAHGAFVASPRFVGIRDLAGEDWRSAFDDAPDALVSYPLLLAADG
                     SSRVVAGAHWLANRSFVAADGAGRIILGTTADAFFSLDRLAGFLRESPLNLTLALNLD
                     GGPVACQGIALNGYRRDFCGSWELAERDGALKLWQPLLGKGRAALPIVLAVLPK"
     gene            5797..6549
                     /locus_tag="VGP50_08215"
     CDS             5797..6549
                     /locus_tag="VGP50_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027402.1"
                     /translation="MGDFILETEGLTKEFAGFTAVRGVDLKIGRGTIHALIGPNGAGK
                     TTVFNLLTKFLEPSAGRILYKGHDITRHRAADVARLGMVRSFQISAVFPHLTVRENLR
                     VALQRPLGISFHFWRSAKSLRALDPRADALMAAVGLADDAEIPATELPYGRKRALEIA
                     TTLALDPEMLLLDEPMAGLGHEDIGRISGLIKDVARQRTVLMVEHNLSVVADLCNRIT
                     VLTRGHVLAEGSYAEVSANAEVREAYIGTGNA"
     gene            6542..>6725
                     /locus_tag="VGP50_08220"
     CDS             6542..>6725
                     /locus_tag="VGP50_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8027403.1"
                     /translation="MPDAAAPPSEMLRIEGLEAWYGESHVLHGMSFAVEQGQVVTLLG
                     RNGVGKTTTLRAVMGIL"
BASE COUNT         1098 a         2363 c         2177 g         1087 t
ORIGIN      
        1 ccccatgccc gggaagaccg cttctttcgg gatggtcttt cccgagcatc cgcagcgcgt
       61 cttcgcggcg gcggagctct ccgacgggac tctgcgctat ctcgctttgg ccggcgcgct
      121 gctcgcttat cgcctgccgg ccttcgtcgc cctcaacgag cccgagacga gcctccatcc
      181 cgagctgctg ccgctgctgc cggcgctggg acggctgatc gcccgcgccg cttcgcgcac
      241 gcaggtgtgg gtggtcaccc attcggagat cctggcaagc gccatcgccg cgcatggcaa
      301 tgtggcgccg ctgacggtga tcaagcgcga cggcgagacc tggatcgagg gcctgacgct
      361 cgcgggcgaa ttccgcgaag acgaggagtg atcccgctag ccggcgctct ccgaggattt
      421 cggcatcttc ccggcgatgc cgaggatgtc ctcgcgccgc tccatctcct cccacacctc
      481 gaccggccag acgcccagag cggcccacag cacgacgagg tgatagagga gatcggcgct
      541 ctcggcgatg acggccgcgc ggtcgttttt caccgtgtcc aacgcgacct ccaccgcctc
      601 ttccgcaagc ttcttggcga tctggctgct gcccgcccgc agcaatttgg ccgtgcgcgg
      661 gaactggaga ccgtcgctgc cgcgggaaag gactgcggcg tggagccgct cgatcgccga
      721 ccggctcaac ttggctcctc cgcgattccg ccggcaggct cctggcccgg cgggatcgcc
      781 gccgctgcct ccgcctgcgc gtcccaatgc ctgagccggc tcggctcgag ttcgcccgtc
      841 gtctcgagga ccggataggc cacggagcag atatgcgagt ggatgcgctt caagtcgcgc
      901 aggatgtcga ggtggagcga gctcgtctcg acgctttccg ggcgcccctc gcgcagccgg
      961 cccagatggc tgtcggcggc cgcgcgttcc gcttcgcgaa tatgaacctt ctcgcccagc
     1021 agccggcgcg cgatcgcgac atcgcccgac atgaacacgc cgagcgccag cttcaggttg
     1081 tccagcacct ggcggtgcag cgcctccagc tccgcgcttc cctcggcaga gaatttgagc
     1141 cggttcttga tcttcttccc ggcgagctgc atcaggttct tgtcgatgat gtcgccgata
     1201 tgctcgaggt tgatcgagaa ggcgatgatc tcgatggcac gtctggcgct ctgctcgtcg
     1261 aggctctccc gcgtcagctg cgtgacatat tgcttcaccg cctcgtacaa tttgtcgacc
     1321 acattgtcca ttctttcgat ctcggcgacg agcttgcggt cgtccgacag gaaggcggtc
     1381 atcgtccggc tcagcatgct ctcgacgatg tcgcccatat gcagcacttc gcgcgcggca
     1441 ctggtgagcg ccagcgtcgg cgtcgagagc acggagggat cgagatagag cggcgcggcc
     1501 ggatccgcgg ccttcttccg ctccggaaac agaaaggtca gcagacgcgc atagctgcgc
     1561 agcggcagaa tgaacaatgc cgccatgacc aggttgaagg ccgtatggaa atcggccacc
     1621 tgccgaaccg gactcggatc gacgacggcg agagcatcgg cgatcggacc gaggaagggc
     1681 agaacgatgg cgcagccgac gagccggttg atcaggtttc cgaccgggac ccgttgcgac
     1741 gccgggttgc ttccgctggc ctccatgacc gggttgagcg cgctgccgag attggcgcca
     1801 agggcgagcg ccagcgccgc ggctggcgtc acgaaatgag agaagctcag cgacatcacc
     1861 acgaccacgg cggcgacgct cgaatgggcc gcccaggcga aaagggcgcc ggcgagcaga
     1921 ttgagcaccg gttcccccgt gatccccgcc aagaccgcgc ggacggccgg cgcatcctcg
     1981 gccggggcca gcgtatccag caggatatgg agggacagca ggatcaggcc gaggccgatc
     2041 gccacgcggc cgagatcgcg gatgcgcgtc ttgggtgccc gcttgaaggc gagaacgccg
     2101 cccgccagca aaacgggcgc cagcgacatg acattgaagg acaggagctg caccagcagc
     2161 gtcgtgccga cattggcacc gagcatgatg gcgagcgcgg gcacgagggc gatgaagccg
     2221 ccggccgcga aggaggagac catcagcgcg gtcgccgtgc tgctttgcag gacggcggtg
     2281 acggcgaggc cgaaggcgaa gccgcggaag cggttgctca gcgccttgcc gaggaaccag
     2341 cgcaggtcgc cgccgaaggc gcgcagcacg ccgctctgga ccatgtgcag gccccagagg
     2401 agcagtgcga cgccgccgat cagatccagc aggatcatcc ctgccatgac tcgtccttcc
     2461 tcgtcgacga cagaacctcg gcgaatctta ggcccgggct tgcagggata acagcgcttt
     2521 ctgccgattc gatcgacgga tcgccgggat atcgggtgat atcgacggtt accgcgacac
     2581 ggaacgcggg agtatcgccc ggcggatcac tgctcgatga tgcggaagcg caccatggcg
     2641 tcgtcgatgg tggcgcgggc gcgcgactgc cacacgtcat aggcgtcttt ataggtcgcg
     2701 aacggaccgt gacgctcctc gcgcgcgccg ggcacgagcg tcttgaagct cagatccgtg
     2761 tactcgccgc cgatcaccca atacatacgg cgctcgtcgt ccctgtggtg gcccgatgcg
     2821 gcggagcgac ctatagtcag cgcccttcct cgacgcaagg tagccgatgc cttacgcttc
     2881 gcttcgcgat ttcatcgccc gcctcgaagc ggcggagcgg ctcgtgcgcg tcaggacgcc
     2941 ggtctcgccg gtgctggaga tcaccgagat ccacacgcgg cttctcgccg agaaagggcc
     3001 ggcggcgctg ttcgaatcgg tatcgcggcc cgagggcggg cgctacggca tgcccgtgct
     3061 caccaacctc ttcggcaccg tcgagcgcgt cgcctggggc atggaccgcg agccgcatca
     3121 gctgcgcgaa atcggcgaga cgctcgcttt cctgaagcag cccgagccgc ccggcggctg
     3181 gcgcgaagcg ctcgagatgc tgccgctgct caagaccgtg ctggcgatga agcccaagac
     3241 cgtcggcagc gctccctgcc aggacgtcgt gctcaccggc accgacatcg atctcggccg
     3301 cttccccatc cagacctgct ggccgggcga gccggcgccg ctcatcacct ggccgctcgt
     3361 ggtcaccaag ggccccggca agcggcgcga ggacgactac aatctcggca tctatcgcat
     3421 gcaggtgatc ggccgcgaca cgacgttaat gcgctggctc aagcatcgcg gcggcgcgca
     3481 gcatcaccag cgctggtcgc agcagaagcg cgagccgctg ccggccgcgg ccgtgatcgg
     3541 cgccgacccc ggcaccatcc tcgccgcggt gacgccggtg cccgacacgc tctcggagta
     3601 tcagttcgcc ggccttttgc gcggtcgcaa agtcgagctc gtcgattgcc gcaccatccc
     3661 gctcaaggtg ccggcgcagg ccgagatcgt gctcgagggt catgtgtcgc tcgacgacga
     3721 ccgcgacgaa ggcccttacg gcgaccacac cggctactac aacgcggtcg agcgctttcc
     3781 ggtgttccgc atctccgcca tcaccatgcg ccgcgacccg atctatctca ccaccttcac
     3841 cggccgtccc ccggacgagc cttcggtgct gggcgaggcc ctcaacgagg tgttcatccc
     3901 gctgctcacg cagcaattcc ccgagatcgt cgatttctgg ctgccgcccg aaggctgctc
     3961 ttaccgcatc gccgtagtgt cgatgaagaa ggcctatgcc ggccacgcca agcgcgtgat
     4021 gatgggcatc tggtccttcc tgcgccagtt cgtctacacg aaattcgtca tcgtcgtcga
     4081 cgacgacatc gacgcgcgcg actggaagga cgtgatgtgg gcggtgtcga cgcgcatgga
     4141 tccggcgcgc gacatcaccg tcatcgacgg gacgcccatc gactatctcg acttcgcctc
     4201 gcccgaagcc ggcctcggcg gcaaaatcgg cctcgacgcc accaacaaat ggccgggcga
     4261 gaccaagcgc caatgggggc gcaagatccg catgaccgac gacgtcatcg ctcaggtcac
     4321 cgccaaatgg ccaaccctcg gcctccccgg caccggcaaa gccatctgga agtgacggag
     4381 agcagcaggc gtacttaccc ctcgcccgca tcgcgggaga gggaggggcc cactgcgaag
     4441 cttcagcgaa gcagtgggag ggtgagggaa tgcccaccac cacccttacc cgacgcgggc
     4501 ttcgcgcgct gtcagatcaa gaatggacct cgccgaggac gcggatgcgg ccctgcgttc
     4561 tattacacgc ctatcgacca tctcgacttc gcgttgcccg aaactgcaac cgggcgacag
     4621 gttttcatcg ctcgctcacg ccgccgcaat ctcgccacgg acggctccca ccatgagcgc
     4681 gtcgggagca tggatcggcc atggcacgcg gcttgcgctc tgcgctcaag aaacatccgc
     4741 tgatgatgat ggcaggcgcc ctcatgatcg cggcaaccct cgccgccgcc gcgttctacg
     4801 cctatgccgg cgcctacggc ttcaacgtcg tgctgcggcg cggctcgacc atctgggtca
     4861 ccgtccgcgc ggacgacccg cgcctctcgc cgtcgatgcg ccgggccttg cagcagcccc
     4921 cgccgctcgc gacacccggg ccgttcgcct gggacgacgt ggccgccggt ttcgcggtgg
     4981 gcgagctccc tgttctcgcc gacggccacg aggtcgatcg cctcctcctc gcccgcatcg
     5041 acccgcagcg cttccgcttc gtgctgcgga acgcgccggc cggcaaccgc gcgctcggca
     5101 actggatggc cgcgctccac gccgtgctcg tcgtcaacgg cagctacttc gcgacggacg
     5161 ggacgccggc gacgccgttt cgcagcgacg gcatcgccct gggcccgcgc gattacaagg
     5221 ccgcccatgg cgcgttcgtc gcgtcgccgc gtttcgtcgg catccgcgat ctggccgggg
     5281 aagactggcg ctcggccttc gacgatgcgc cggacgccct cgtctcctac ccgctccttt
     5341 tggccgccga cggctcgagc cgcgtcgtgg cgggcgcgca ttggctcgcc aaccgcagct
     5401 tcgtcgccgc ggacggcgcc ggacgaatca tcctcggcac gaccgcggac gcctttttct
     5461 cgctcgaccg cctcgccggc tttcttcgcg aatcaccatt gaatctgacg ctcgcgctca
     5521 acctggacgg ggggcccgtc gcatgtcagg gcatcgcgct caacggatat cggcgcgact
     5581 tctgcggcag ctgggagctc gccgagcgcg acggcgcctt gaagctgtgg cagcccctcc
     5641 tcggcaaagg gcgcgccgcc ctgccgatcg tgctcgcggt gctcccgaaa tagccgcggc
     5701 ttagcttgtc agcgttccgt cgcgccgtta gcctctaagc gggcgcgtta acaaggaagg
     5761 cgggcgacgc acccgccttc gtcggggagt gcaggcttgg gcgacttcat cctggagacg
     5821 gagggtctca ccaaggagtt tgccggcttc accgcggtgc gcggcgtcga cctcaagatc
     5881 gggcgcggca cgatccatgc gctgatcgga cccaacggcg ccggcaagac caccgtcttc
     5941 aacctcctca cgaagttcct cgagcccagc gccgggcgca tcctctacaa agggcatgac
     6001 atcacccgcc accgcgccgc cgacgtggcg cgcctcggca tggtccgctc ctttcagatc
     6061 tcggcggtgt tcccgcatct gacggtgcgc gagaatctgc gggtcgcgct gcagcggccg
     6121 ctcggcatct ccttccactt ctggcgctcg gcgaaaagcc tccgcgcgct cgatccgcgc
     6181 gccgatgcgc tgatggccgc ggtcggcctc gccgacgacg ccgagattcc cgcaaccgag
     6241 ctgccttacg gccggaagcg cgcgctcgag atcgcgacca cgctggcgct cgaccctgag
     6301 atgctcctcc tcgacgaacc catggcaggg ctggggcacg aggatatcgg ccgcatctcc
     6361 ggcctcatca aagacgtagc gcgccagcgc accgtcttga tggtcgagca caatctctcc
     6421 gtcgtcgccg atctctgcaa ccggatcacg gtcctgacgc ggggccatgt cctggccgaa
     6481 ggaagctacg ccgaggtctc ggccaatgcc gaagtgcgcg aggcctatat tggcacgggc
     6541 aatgcctgac gccgccgcac cgccctcgga gatgttgcgc atcgaggggc tcgaggcctg
     6601 gtacggcgaa tcccacgtgc tgcacggcat gagcttcgcc gtcgagcaag gtcaggtggt
     6661 gacgctgttg ggacggaacg gcgtcggaaa gaccaccacc ttgcgcgcgg tgatgggcat
     6721 cctgc
//
LOCUS       DASYNS010000263         6286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1466190, whole genome shotgun sequence.
ACCESSION   DASYNS010000263 DASYNS010000000
VERSION     DASYNS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466190"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VGP50_08225"
     CDS             complement(<1..469)
                     /locus_tag="VGP50_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8027404.1"
                     /translation="MLEVRDLHAYYGKSHILQGVNLDVGDGEIVSILGRNGVGRSTTL
                     KAIMGGVPPQGSITFKGRPIAGLKPYEIAHLGLGYVPEDRAIFPTLTVAQNLLLGVKS
                     GRKATRWGLEDGYRLFPQLRERADTPAGVLSGGEQQMLTICRTLMGDPDLVMVD"
     gene            complement(469..1218)
                     /locus_tag="VGP50_08230"
     CDS             complement(469..1218)
                     /locus_tag="VGP50_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027405.1"
                     /translation="MTPALELRDLEKRFGRTEIIRGISLDVPTGERHAIIGPNGAGKS
                     TLFNLISGRLAPSAGTIRLKGEEIAGLHPHQVNRRGLSRSFQVTNIFPSLTVWENVRC
                     AVLWSQGYGYSFWHAVDRLGDVRARTEDILGQINLLDRCRLPAGVLTYAEQRALEIGI
                     TIAGGAELILLDEPTAGMSRGESEHAVALIRKVSEGKTLVMIEHDMGVVFDLADRISV
                     LVYGQIIASDAPSRIKADAAVQSAYLGQAVA"
     gene            complement(1215..2522)
                     /locus_tag="VGP50_08235"
     CDS             complement(1215..2522)
                     /locus_tag="VGP50_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8027406.1"
                     /translation="MSEISKPVLETGLAAAARGMLRDYGIWVATAVVFAALPRIFTSG
                     TALTMMSLMGIMIIFALSYNMLIGQTGMLSFGHAVYYGLGGFLVVHAMNAVAHDHLPV
                     PVPLLPLAGAAAGLFFGFIFGSVSTRRGGTAFAMISLGLGELVASSALILRGFFGGEE
                     GITTNRTKLAPFFGYHFGPQIQVYYLIAAWCFVAMLAMYAITRTPFGRICNAVRDNPE
                     RAEFVGYNPYAVRFIAFCLSGFFAGIAGGLTAINFEIMNSVNVAAGQSGVVLLMTYIG
                     GVGNFIGPVLGAVLITYLQVMLSDVTPVWQLYFGLLFIGMVIFAPGGIAGLLMMHAPL
                     WHAGVLHRVAPAYVLALAPGLMTLAGVSLAIEMTFRLTARGSGDTAMSFLHVPLDAAS
                     LLPWAAAALLAGGGFWLFRRSWPAVGAAWSDALAAARGGSGRA"
     gene            complement(2519..3469)
                     /locus_tag="VGP50_08240"
     CDS             complement(2519..3469)
                     /locus_tag="VGP50_08240"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8027407.1"
                     /translation="MLELIIVSTLNGLLYGMLLFLMASGLTLIFSMMGVLNFAHASFY
                     MLGAFFGFQIAQWLGFWPALLVAPVLVGLIGALIERYGLRMVHRHGHVAELLFTFGLA
                     FVIEEVVQMIWGTAPADYRVPASLDFTAFTIFSTNYPFYKMFELLTAIAIFIALLALL
                     TRTRVGLIIRAALTHPQMVGMLGHNVPRIFMMVFGVGSALAAVAGVIAGPALVTQSNM
                     AELLGPILFVVIVVGGLGSLSGAFVASLLIGLVQTFAIALDISLGQIFGLIGIDIGPG
                     SIFADVWHVTIAQIGPIIPYLLLVLVLIFRPTGLLGTRQT"
     gene            complement(3547..4794)
                     /locus_tag="VGP50_08245"
     CDS             complement(3547..4794)
                     /locus_tag="VGP50_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV8027408.1"
                     /translation="MTWRVSFAAVLVAVAGAAALAAPAAADSLRIAYVDPLSGGAASV
                     GEDGLRHFRYIADQFNAKGGVLGMRVEIVPYDDKLNPQESLVQVQKAIDAGIRIITQG
                     NGSSVAAAIEDFVAKYNDRNPGKEMIYLNYAAVDPALTNEKCSYWHFRWDANSDVKME
                     ALTNYMQSRESIKKVYLINQDYSFGQAVRAEAVKMLKEKRPDLQIVGDELHPLQKVTD
                     FAPYIAKIRASGADSVITGNWGQDFALLVKAAADAGLQVDWYTYYAAGVGGPTAIKQA
                     NLKDRVFEITEGVANDPYAPAQETEKAFRAKLNQSNHYPRAFNQMEMLLKAIEQTKST
                     DPRKIAQALEGMKLAVLNGGEGFMRSDDHQFFQPMYVAVFGPRNPVQPFDEESTDWGW
                     KTVARIDTENTMVPSSCQMAHPS"
     gene            4970..5431
                     /locus_tag="VGP50_08250"
     CDS             4970..5431
                     /locus_tag="VGP50_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV8027409.1"
                     /translation="MRRFADIAELRTAVGQVLGESDWVTVTQETIDLFAEATGDHQWI
                     HVDRERAKASPFGTTIAHGFLTLSLIPRLIYRIYGVGKIAASLNYGSNKVRFITPVPS
                     GGRIRARLKLLSAEDTPRGVRITSEATVELEGSERPACTAELVAVLIPTEA"
     gene            5445..>6286
                     /locus_tag="VGP50_08255"
     CDS             5445..>6286
                     /locus_tag="VGP50_08255"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV8027410.1"
                     /translation="MTLLDESARGVYVISATPFTDAGAMDWASTDRLMDFYLDCGVDG
                     VTILGAMGEAPKLSESEAEAFVTRVLTRIAGRVPVIVGVSSPGLKLLADFARRAMDLG
                     AAGVMVSPATGLKTDDQIHGYVASAVRELATIPVVLQDYPQLTQVFMSASLVNRCFAD
                     FPTLKMLKHEEAPGLRKLSQVRAADAARGRRISILVGNGGIHLPQELARGADGAMTGF
                     AFPEMLVEVCRRFAAGDAEGAEDVYDAFLPVVRHEQQPGIGLALRKETLRRRGILASA
                     RTRAP"
BASE COUNT         1157 a         2048 c         2091 g          990 t
ORIGIN      
        1 cgtcgaccat aacgagatcg ggatcgccca tcagcgtgcg gcagatggtg agcatctgct
       61 gctcgccgcc cgacagcacg ccggccgggg tatcggcgcg ctcgcggagc tgggggaaga
      121 ggcggtagcc atcctcgagg ccccagcgcg tcgccttgcg tccgctcttg acgccgagca
      181 gcagattctg cgccacggtc agcgtcggga agatggcgcg gtcctcgggt acatagccga
      241 gcccgagatg ggcgatctca tagggcttga ggccggcgat cggccggccc ttgaaggtga
      301 tcgagccctg gggcggcacg ccgcccatga tggccttcag cgtcgtcgag cggccgacgc
      361 cgttgcgccc taggatgctg acgatctcgc catcgccgac atcgaggttc acgccttgga
      421 ggatgtggct cttgccgtaa taagcgtgga gatcccgcac ctccagcatc aggccaccgc
      481 ctggccaaga taggcgctct gcacggcggc atccgccttg atccgcgacg gcgcgtcgct
      541 ggcgatgatc tggccataga ccagcaccga gatgcggtcg gcgaggtcga agaccacgcc
      601 catgtcgtgc tcgatcatca ccagcgtctt gccttcgctc accttgcgga tcagcgccac
      661 ggcatgctcg ctctcgcccc ggctcatgcc ggccgtaggc tcatcgagca ggatgagctc
      721 ggcgccgccg gcgatggtga tgccgatctc gagcgcccgc tgctctgcgt aagtcaaaac
      781 acccgccggc agccggcaac ggtcgaggag attgatctgg ccgaggatat cctcggtgcg
      841 cgcccgcaca tccccgagcc ggtcgaccgc gtgccagaag gaatagccat aaccctgaga
      901 ccacagcacg gcgcaacgca cattctccca caccgtcaag ctcggaaaga tgttggtgac
      961 ctggaagctg cgcgagaggc cgcggcgatt gacctggtgc ggatggaggc cggcgatctc
     1021 ctcgcccttg aggcgaatgg tgccggcgct gggcgcgagg cggccgctga tcaggttgaa
     1081 gagggtggat ttgccggcgc cgttcgggcc gatgatggcg tggcgctcgc cggtcggcac
     1141 atcgaggctg atgccgcgga tgatctcggt gcgaccgaag cgcttctcga gatcgcggag
     1201 ctcgagcgcg ggcgtcatgc ccggcccgat ccgccgcgcg ccgcggcgag cgcgtcgctc
     1261 caggccgcgc ccaccgccgg ccagctgcgg cggaacagcc agaagccgcc gccggcgagg
     1321 agcgccgctg ccgcccacgg caggagcgag gcggcgtcga gcggcacatg caggaaggac
     1381 atcgcggtat cgcccgagcc gcgcgccgtc aggcggaagg tcatctcgat cgcgaggctg
     1441 acaccagcga gcgtcatgag tcccggcgcc agcgccagga cataagcggg agccacgcgg
     1501 tgcagaacgc cggcatgcca gagcggcgcg tgcatcatca gaagaccggc gatcccgccc
     1561 ggcgcgaaga tgaccatgcc gatgaagagc aggccgaaat agagctgcca gaccggcgtc
     1621 acgtcgctca gcatcacctg gagataggtg atgagcacgg cgcccagcac cggcccgatg
     1681 aaattgccga cgccgccgat ataggtcatc agcagcacga cgccggactg ccccgcggcg
     1741 acattcaccg agttcatgat ctcgaaattg atggcggtga ggccgccggc gatgccggca
     1801 aagaacccgg agaggcagaa ggcgatgaag cgtacggcgt aggggttgta gccgacgaac
     1861 tccgcgcgct cgggattgtc gcgcacagcg ttgcagatgc ggccgaacgg cgtgcgcgtg
     1921 atcgcgtaca tggcgagcat ggcgacgaag caccacgcgg cgatgaggta atagacctgg
     1981 atctgcggcc cgaaatgata gccgaagaac ggcgcaagct tggtgcggtt ggtggtgatg
     2041 ccttcctcgc cgccgaagaa accgcgcagg atcagcgcgc tcgacgccac gagctcgccc
     2101 aggcccagcg agatcatggc gaaggcggtg ccgccgcgcc gcgtcgacac gctgccgaag
     2161 atgaagccga agaacagccc ggcggcggcg ccggccaggg gcagcaacgg caccggcacc
     2221 ggcagatggt catgcgcgac agcgttcatg gcatgcacga ccaggaagcc gccaagccca
     2281 taatagacgg cgtgaccgaa agacagcatg ccggtctggc cgatcagcat gttgtaggag
     2341 agggcgaaga tgatcatgat gcccatgagg ctcatcatcg tcagggccgt tcccgaggtg
     2401 aagatgcgcg gcagggccgc gaagacgaca gcggtcgcga cccagatgcc gtaatctcgg
     2461 agcatgccgc gcgccgccgc ggcgagtcct gtctcgagca cgggtttcga gatctcgctc
     2521 acgtctgacg ggtccccaaa agcccggtcg gacggaagat cagcacaagc accagcagca
     2581 gataagggat gatcggcccg atctgggcga tggtcacgtg ccagacgtcg gcgaagatcg
     2641 acccgggacc gatgtcgatg ccgatgagcc cgaatatctg gccgagcgag atgtcgagag
     2701 cgatggcgaa ggtctgcacc agcccgatca ggagcgaggc gacgaaggcg cccgagagcg
     2761 agccgaggcc gcccacgacg atgacgacaa acaaaatcgg cccgagcagc tccgccatgt
     2821 tggactgcgt caccagcgcg gggccggcga tcacgccggc gacggcggcg agcgccgagc
     2881 ccacgccgaa gaccatcatg aagatgcgcg gcacgttgtg gccgagcatg ccgaccatct
     2941 gcggatgggt gagcgccgcg cggataatga ggccgacccg cgtccgcgtg agcagcgcca
     3001 gcagcgcgat gaagatggcg atcgccgtca gcagctcgaa catcttgtag aagggatagt
     3061 tggtcgagaa gatggtgaag gcggtgaagt cgagcgatgc cggcactcgg taatccgccg
     3121 gcgccgtgcc ccagatcatc tgcacgacct cttcgatcac gaaggcgagg ccgaaggtga
     3181 agagcagttc cgccacatgg ccgtggcgat ggaccatgcg caggccatag cgctcgatca
     3241 gcgcgccgat gaggccgacg agcaccggcg ccaccaatag cgccggccag aatccgagcc
     3301 actgcgcgat ctggaaaccg aagaacgcgc cgagcatgta gaagctcgca tgggcgaagt
     3361 tgagcacgcc catcatgctg aagatcagtg tgagcccgct cgccatcaaa aagagcagca
     3421 tgccgtagag caggccgttg agggtcgaga cgatgatgag ctcaagcacg cgtcaaccgc
     3481 cccgaagatc gcttcacgcc gctccggcgc atcctggcca gagcggccgc cggcatatcc
     3541 cttcgatcag ctcggatgcg ccatctggca actgctggga accatcgtgt tctcggtatc
     3601 gatccgcgcc accgtcttcc agccccaatc agtgctctcc tcgtcgaagg gctggaccgg
     3661 gttgcgcggc ccgaacaccg cgacatacat cggctggaag aactggtggt cgtcggagcg
     3721 catgaagccc tcgccgccat tgagcacggc gagcttcatc ccctcgagcg cctgagcgat
     3781 cttgcgcgga tcggtcgact tggtttgctc gatcgccttc agcagcatct ccatctgatt
     3841 gaaggcgcgc ggatagtggt tgctctgatt gagcttggcg cggaacgcct tctcggtctc
     3901 ctgcgcgggc gcataaggat cattggcgac gccctcggtg atctcgaaga cgcggtcttt
     3961 gaggttggcc tgcttgatcg ccgtcggccc gccgacaccc gccgcatagt aggtgtacca
     4021 gtcgacctgg aggccggcat cggccgccgc cttcaccagc agcgcgaaat cctggcccca
     4081 attgccggtg atgacgctgt cggcgcccga cgcacggatc ttcgcgatat agggcgcgaa
     4141 atcggtgacc ttctgtagcg gatgcagctc gtcgccgacg atctgcagat cggggcgctt
     4201 ctccttcagc atcttgaccg cttcggcgcg caccgcctgg ccgaaagaat agtcttgatt
     4261 gatgaggtac acttttttga tgctctcgcg cgactgcata tagttggtca gcgcctccat
     4321 cttgacgtcg gagttcgcat cccagcggaa atgccaatag ctgcatttct cgttggtgag
     4381 cgcgggatcg acggcggcat agttgaggta gatcatttct ttgccgggat tgcggtcgtt
     4441 gtatttggcg acgaaatcct cgatcgccgc ggcgacactc gacccgttgc cttgggtgat
     4501 gatgcggatg ccggcgtcga tcgccttctg cacctgcacc aagctttcct gcgggttgag
     4561 cttgtcgtca taggggacga tctcgactcg catgccgagc acgccaccct tggcattgaa
     4621 ctgatcggcg atgtaccgga aatgcctcaa gccatcctca ccgacgctgg cggcgccgcc
     4681 ggagagtgga tcgacatagg caatcctgag gctatcggcg gctgcgggcg ccgccagggc
     4741 ggccgcgccg gcaacggcca ccaggaccgc ggcaaaactc acgcgccaag tcattccaac
     4801 ctcccttgcc agcgtctgat tcgctgcctt agaccggtgg cagcgatgac acattttctt
     4861 atttcaaagg tcgcccaccc cccccatgaa gtcaagcgcc gggtgccgcc gcgctggacg
     4921 cgcccgccgc ggcagggaac aatgccaccg ccaagacacg ggagggggga tgcgccgctt
     4981 tgccgatatc gccgaactcc gcactgcggt cggccaggtc ctgggcgaga gcgactgggt
     5041 gaccgtcacg caggagacga tcgatctctt cgccgaggcg accggcgacc accaatggat
     5101 ccatgtcgac cgcgagcggg cgaaggcctc gcccttcggc accaccatcg cccacgggtt
     5161 tctgacgctg tcgctgatcc cgcgcctgat ctatcgcata tacggcgtcg gcaagatcgc
     5221 ggcgagcctc aactacggca gcaacaaggt acgcttcatc acgccggtgc cgagcggcgg
     5281 ccgcatccgc gcccgcctca agctgctctc tgccgaggac acgccgcgcg gcgtgcgcat
     5341 cacctcggag gcgacggtcg agctcgaagg atcggagcgc cccgcctgca ccgccgagct
     5401 cgtcgccgtc ctgatcccga ccgaggcatg agcaaggaga tgttatgacg ctcctcgatg
     5461 aatccgcccg cggcgtttat gtgatctcgg ccacgccctt caccgatgcc ggcgccatgg
     5521 actgggcgtc gaccgaccgg ttgatggatt tttatctcga ttgcggtgtc gacggcgtga
     5581 ccatcctcgg cgcgatgggc gaggcgccca agctttcgga gagcgaggcc gaggccttcg
     5641 tcacgcgcgt gctgacgcgc attgcgggcc gcgtccccgt gatcgtcggg gtgagcagtc
     5701 ccggcctcaa gctgctcgcc gacttcgccc gccgcgccat ggatctgggc gccgcgggcg
     5761 tcatggtctc gcccgccacc ggcctcaaga cggacgacca gatccatggc tatgtcgcga
     5821 gcgccgtgcg ggagctcgcc acgatcccag tggtgcttca ggattatccg caactcaccc
     5881 aggtgttcat gtcggcaagc ctcgtcaatc gctgcttcgc cgatttcccg acgctcaaga
     5941 tgctgaagca tgaagaggcg ccgggcctgc gcaaactctc gcaggtgcgc gccgccgatg
     6001 cggcgcgcgg ccggcgcatc agcatcctcg tcggcaatgg cggcattcac ctgccgcagg
     6061 agttggcgcg cggcgccgat ggcgcgatga cgggcttcgc cttcccggag atgctggtcg
     6121 aggtctgccg ccgcttcgcc gcgggcgatg ccgaaggtgc cgaggacgtc tacgacgcct
     6181 ttctcccggt ggtgcgccat gagcagcagc cggggatcgg ccttgcgctc cgcaaggaga
     6241 cgctgcgccg gcgcggcatc ctcgccagcg cgcgcacgcg ggcgcc
//
LOCUS       DASYNS010000264         4199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3651853, whole genome shotgun sequence.
ACCESSION   DASYNS010000264 DASYNS010000000
VERSION     DASYNS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3651853"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..985)
                     /locus_tag="VGP50_08260"
     CDS             complement(143..985)
                     /locus_tag="VGP50_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8027411.1"
                     /translation="MNKKMLALYGLKWNPFAPDVPVEALHVSRRIESFCWRVEQLVGE
                     GGFALVTGAPGTGKSVTLRILAERLGGQRDVKIGIVSRPQAGLADFYREMGDLFGVEL
                     APHNRWAGAKVLRDRWQSHIDAALSRPVLIVDEAQEMLPAVLAELRLLSSARLDSHIL
                     LTVVLGGDGRLLERLRSEELAPLGSRMRVRLALERATPDELQELLLHGLQKAGAVKLM
                     TPELIATLADHAQGNPRALMTIAGELLAVAAQREARHIDEKLFLETCAPPAAETKAAS
                     ARRR"
     gene            complement(982..2460)
                     /locus_tag="VGP50_08265"
     CDS             complement(982..2460)
                     /locus_tag="VGP50_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEV8027412.1"
                     /translation="MIQQARGSRVHERWAHLRFSVIGQLLAAPPAKGELKAAIMALAA
                     RTWQHPITGDPVQFGFATIERWYYRALKERADPVGVLRRKLRADAGRQVAIGDVVRQA
                     VLAQYAAHRSWSVQLHHDNLVALAETRPELKPVPSYATLRRFMSHNGLAKRRRVTARR
                     TDGAERAEARIADREVRSYEAEYVNALWHWDCHHGSRKVLTQRGEWVTPILFGVLDDR
                     SRLACHLQWYLAETAEVIAHGLSQAFQKRGLPRSALSDNGAAMTAAEIAEGLTRLGVL
                     HHTTLPYSPYQNAKQEAFWGPVEGRLIAMLEHVPDLTLTFLNEATQAWVEHEYNRKPH
                     AEIGEAPISRFLAGPEVTRPCPDSDALRLAFTRSAHRTHKKSDGTIVIEGRRFEVPNR
                     YRHLNRIEVRYASWNLGLVHLVDERTGHVLARLYPQDKTQNASGLRRSLDPVSSASPA
                     AEPPAVTPASTIAPLLARLLDRQAAAGLPPAYLPKNEEGDDT"
     gene            complement(2550..3104)
                     /locus_tag="VGP50_08270"
     CDS             complement(2550..3104)
                     /locus_tag="VGP50_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027413.1"
                     /translation="MADATFHQLLLACDADLADAARAGRCALCGGALHSACYPRKPRG
                     RPCRLGPEHDRRFSFCCAVDGCRSRATPPSLRFLGRKVYLAAIVALVAIMRHGVTTSR
                     MEHLSQAVGVDRRTVERWRMWWRDSFTVTPFWRVARAAFMPPIDRERLPASLLERFTG
                     DVADRLVALLRFIGPVTGGRVHAR"
     gene            3158..3700
                     /locus_tag="VGP50_08275"
     CDS             3158..3700
                     /locus_tag="VGP50_08275"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEV8027414.1"
                     /translation="MAKPAPKPGRNDPCHCGSGNKYKKCCLAKDEAAERDSLVKAQAR
                     REQPIVAHRHERAAAHRLPVDELMAEVAARLSMAEKEDAYEDELDAASNAVVRLVRAA
                     RLDEAEAAARDLLVRFPDVHDGWDRLGMVHEARGDSRQAADCYRKVIDFIRQHPDDYD
                     AEVAEQFANLVEKLDPPLAT"
BASE COUNT          702 a         1395 c         1450 g          652 t
ORIGIN      
        1 tcgagggcga ggaacgactt gcagcacttg ggctcgccgc cgacgatgcc gacggcctgc
       61 tcggaccaca ggccggcaat gagccagcgg tgctcctcct tgcgctcggc gaggcgccat
      121 gcgggctcga cgggcagcag gctcatcggc gccgtgccga tgccgccttg gtctcggcgg
      181 cgggcggagc gcaggtctcc aggaacagct tctcgtcgat atggcgggcc tcgcgctgcg
      241 ccgcgaccgc gagcagctcg ccggcgatgg tcatcaatgc gcgcgggttg ccctgggcgt
      301 gatcggcgag cgtggcgatc aactccggcg tcatcagctt gacggcgccg gccttctgga
      361 ggccatgcag cagcagttcc tgcaactcgt ccggcgtagc gcgctccagc gccaggcgca
      421 cccgcatgcg gctgccgagc ggggcaagct cctcggagcg caggcgctcc agcagccgac
      481 catcgccgcc gagcacgacg gtcaacagga tgtggctgtc gaggcgggcg gatgagagca
      541 gccgcagctc ggcgagcacg gcgggcagca tctcctgggc ctcgtcgacg atcagcacgg
      601 gtcgcgacag tgcggcgtcg atatggctct gccagcgatc gcgcagcacc ttggcgccgg
      661 cccaccggtt gtgcggggcg agttcgacgc cgaacaggtc gcccatctcg cggtagaagt
      721 cggcgaggcc ggcctggggc cggctgacga tgccgatctt gacgtcgcgc tgtccgccga
      781 gccgctccgc gagaatgcgc agcgtgaccg acttgccagt gccgggcgcg ccggtgacga
      841 gggcgaagcc gccctcgccg acgagctgct cgacgcgcca gcagaacgac tcgatgcgtc
      901 ggctgacgtg gagcgcctcg accggcacgt cgggagcgaa cgggttccac ttgaggccat
      961 agagggccag catcttcttg ttcacgtgtc gtctccttct tcgttcttcg gcagataagc
     1021 cggcggcagg ccggcagcgg cctgccggtc gagcagacgc gcgagcaatg gcgcgatggt
     1081 ggacgccggc gttaccgcgg gcggctcggc ggcgggcgaa gcggaggaga ccggatcgag
     1141 cgaccggcgc aggccgctgg cgttctgcgt cttgtcctgc ggatagagac gcgccagcac
     1201 gtgcccggtc cgctcgtcga cgagatggac gaggccgaga ttccagctgg cgtagcggac
     1261 ctcgatccgg ttgagatgcc gataacggtt cggcacctcg aagcggcgcc cctcgatgac
     1321 gatggtgccg tcgctcttct tgtgggtgcg atgagcgctg cgcgtgaagg cgaggcgcag
     1381 cgcatcgctg tcgggacacg gccgtgtcac ctcgggaccc gccaggaagc gcgagatcgg
     1441 cgcctcgccg atctcggcgt ggggcttgcg gttgtactcg tgctcgaccc aggcctgggt
     1501 ggcctcgttc aggaacgtca aggtcaggtc cggcacatgc tccagcatgg cgatcagccg
     1561 gccctcgacc gggccccaga acgcctcctg cttggcattc tgataggggc tgtaaggcaa
     1621 cgtggtgtgg tgcaggacgc ccaaccgcgt gagcccctcg gcgatctccg ccgccgtcat
     1681 cgcggcgccg ttgtcgctga gcgccgagcg tggcaggccg cgcttctgga acgcctggct
     1741 gagcccgtgc gcgatgacct cggcggtctc ggccagatac cattgcaggt gacaggcaag
     1801 ccgcgatctg tcgtcgagga cgccgaatag gatgggcgtt acccactcgc cccgctgggt
     1861 gagcaccttg cgcgagccgt gatggcagtc ccagtgccac agagcgttga cgtactcggc
     1921 ctcgtagctg cgcacctcgc gatcggcgat gcgggcctcg gcacgctcgg caccgtcggt
     1981 tcggcgcgct gtcacgcgcc gccgcttggc gagaccgttg tggctcatga accggcgcag
     2041 cgtggcgtag gacggcaccg gcttgagctc gggcctcgtc tcggccagcg cgacgaggtt
     2101 gtcgtgatgg agctggacgc tccagctcct atgcgcggcg tattgcgcga ggacggcttg
     2161 gcgcactaca tcgccgatcg caacctgccg tccggcgtcg gcgcgcagct tgcggcggag
     2221 cacgccgacc ggatcggcgc gctccttcaa ggcccggtaa taccatcgct cgatggtcgc
     2281 gaagccaaac tggaccggat cgccggtgat cggatgctgc catgtgcggg cggcgagcgc
     2341 catgatcgcc gccttcagtt cgcctttggc aggtggcgcc gccaacagct ggccgatcac
     2401 ggaaaagcgc agatgcgccc accgctcgtg aacacgtgaa cctcgtgcct gctggatcat
     2461 gccggcgctc ctgcgatcgg acaacaccga ccgtagaagc agccgcaaca cgctggcgac
     2521 gagcatcctg tgcggatgtc ggaacgccgc tatcgagcgt gcacgcggcc tccggtaacc
     2581 ggtccgatga agcgcaacag cgcgacaagg cggtcggcga cgtcaccggt gaagcgttcg
     2641 aggagcgatg ctggcagacg ctcccgatcg atcggcggca tgaacgcggc gcgagccacc
     2701 cgccagaacg gcgttaccgt gaagctgtcg cgccaccaca tccgccagcg ctccacggtg
     2761 cggcggtcga cgccgacggc ttgcgacagg tgctccatcc gggaggtcgt cacgccgtgc
     2821 cgcatgatcg ccaccagcgc cacgatcgcg gcgagataga ccttgcggcc gaggaagcgc
     2881 agcgagggcg gcgtcgcccg cgaccggcag ccgtcgaccg cacagcagaa gctgaagcgc
     2941 cggtcgtgct caggcccgag ccggcagggc cggccccgcg gcttgcgcgg ataacaggcg
     3001 gaatgcagcg cgccgccgca aagcgcgcat cgtccggcgc gcgccgcgtc ggccagatcg
     3061 gcatcgcagg cgagcaacaa ctgatggaat gtggcgtcgg ccaacaggtc tcgatacaac
     3121 ggatgcgccc tccctcgtcg cgacggtgcc gaccaccatg gccaagcccg ccccaaagcc
     3181 cggacgcaat gatccctgcc actgcggcag cggcaacaaa tataagaagt gctgtctagc
     3241 caaggacgag gcggccgagc gcgatagcct tgtcaaagcc caggcgcggc gcgagcagcc
     3301 catcgttgct catcgccatg agcgcgccgc ggcacatcgc ctgcccgtcg atgagttgat
     3361 ggcagaggtc gccgcgcgcc tcagcatggc cgagaaggaa gacgcctatg aggacgagct
     3421 tgatgccgcc tccaatgccg tggtgcgcct cgtccgcgcc gccaggctcg acgaggccga
     3481 ggcggccgca cgcgacctgc tggtgcgctt ccccgacgtg cacgacggct gggaccgcct
     3541 cggaatggtg cacgaggcgc gcggcgacag ccgacaggcg gccgactgct atcgcaaggt
     3601 gatcgacttc atccgacagc atcccgacga ctacgacgcc gaagtcgccg aacagttcgc
     3661 caaccttgtt gaaaagctcg atccccccct ggcgacctga ccgttccgct ttgctcgcac
     3721 tccgcgaggg cctcggccat cccaccatcc gcgaccgtga ttagattgcc cagcccccac
     3781 cctcagatag cgtgatcagg cttaggcgac ggcggttccg ggtaattgag ggcggtaaac
     3841 aatgaaggtt gcgatggttc gctcagatgt taaaattggg tagcgtcatc ggtggagcga
     3901 gtggttgttg ccggctgact gaaaatcggc caaaggggcc agcccctttc gtgggttcga
     3961 atcccacccg atccgccaag gggggctgcg caagcaagcc cccttggggt ttatagagac
     4021 gccttatttt gcggcggctg gcgtcgtctg atcggcgaat aaatccaatt cgccgatcgc
     4081 cggcatgagg tcgttatgtt cctcaatgcg cagcatccga atgtcagtga gcactttggc
     4141 aaaccaaggg tagcgctttt ggaccgcctg gactgccatg acgcagatta ccggcgtta
//
LOCUS       DASYNS010000265         7737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1779250, whole genome shotgun sequence.
ACCESSION   DASYNS010000265 DASYNS010000000
VERSION     DASYNS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1779250"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VGP50_08280"
     CDS             <1..450
                     /locus_tag="VGP50_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEV8027415.1"
                     /translation="PPSCAEALARSLEMALDDLTRRYGAEMKDWRWGRAHEAAFTNQL
                     LSRVPVLRGIFATRIAADGGDDTLNRAATFTWDEKEPFADVHGPTLRMIVDLSDIAAT
                     RFMIAPGQSGNPLSPHYADLMGRWREVGYLTLDGGAEGGTLELTPAP"
     gene            447..1682
                     /locus_tag="VGP50_08285"
     CDS             447..1682
                     /locus_tag="VGP50_08285"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEV8027416.1"
                     /translation="MTALPVTLADIRAAAAAIAGTVLRTPSVSAPALGNLLGAEVVLK
                     LETLHPTGSFKERGALYKLLSLTPEERRAGVIAMSAGNHAQGLAYHARRLGIPVTIVM
                     PEGTPFTKIERTESYGATVVLRGAGLGEAKQAAAALAAAGHLTLVHPYDDARIIAGQG
                     TIALELLADHPELDTLVVPVGGGGLIAGIAIAAKALKPEITIIGVETTLFPSMLHALG
                     RCAAPPCGQTLAEGIAVLEPGQFTRPVVAALVDEILLADETALERAVALLLDVAKLVV
                     EGAGAAPLAALMSERERCRGRRIGLVLSGGNIDARLLASILQRGLVRAGRLVRLRIEI
                     SDVPGALSKIAGIIGEQGGNIVEVHHQRLFHDVPVKLAEVDAVIETRNPIHVGELVAA
                     LIAAGFPTRLLSSTAMDTR"
     gene            1720..2439
                     /locus_tag="VGP50_08290"
     CDS             1720..2439
                     /locus_tag="VGP50_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8027417.1"
                     /translation="MIEEARRTAIVVGASSGIGEALARQLTAEGWRVCLMARRLSRLE
                     AIAETLSRDALARHIDLSDAETAAATLTAVIDELGGADLVVMSSGTGYPNTALDWPPD
                     RETLAVNVVGFAAVAQAAMRHFIARGRGHLVGITSIAALRGFAPTAAYAGSKALQSLY
                     LESLRDLARLRRLPIAVTEAQPGFVATAMMQSERVFWVASAAVAAQQIMRAIRRRRKH
                     AYITRRWAIIALLLRCLPRPG"
     gene            complement(2436..2729)
                     /locus_tag="VGP50_08295"
     CDS             complement(2436..2729)
                     /locus_tag="VGP50_08295"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEV8027418.1"
                     /translation="MTAYVIAEIDVTDRDAYKIYETAGADSVARHGGRLLARGGRSAG
                     LEGAAPKRIMVIEFATMEAAQRWHDSPDYRDARAIRAIAGKSRLVIVDGAPGA"
     gene            2888..4252
                     /locus_tag="VGP50_08300"
     CDS             2888..4252
                     /locus_tag="VGP50_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEV8027419.1"
                     /translation="MIVHQNVLDSIGNTPLVELTRFDTGRCRLFAKLENQNPAGSIKD
                     RIGLSLIEAAERDGKVAPGGTLIEATAGNTGLGLALVAAQKGYRLILVIPDKMSQEKV
                     LHLKALGAEVRITRSDVGKGHHDYYQDMAERLAAEMGAFYVNQFANPANPLAHETTTG
                     PEIWRQMGHQLDAVVVGVGSGGTLTGLARFFERVAPAAEMVLADPEGSILAPLVRTGR
                     KVEAGSWLVEGVGEDFVPPNCDLSRVRTAYAVSDRDSFAAARDLLRREGILGGSSTGT
                     LLAAALRYCREQTEAKRVVTLVCDSGNKYLSKMYNDFWMFDQGLLTRSEQGDLTDLIA
                     RRHAERSVVTVTPEDTLITVHRRMKLYEVSQLPVLEGERLVGIIDESDLLLAVNADPA
                     AFREPVRRAMVTRVETVAPDAPIAALLPIFAKDHVAVIEKDGKFLGLITRIDFINHLR
                     RRVR"
     gene            4267..5439
                     /locus_tag="VGP50_08305"
     CDS             4267..5439
                     /locus_tag="VGP50_08305"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV8027420.1"
                     /translation="MTPTKSNRRGFATRGIHAGQKPDPSTGAIMTPIYATSTYVQESP
                     GVHKGYEYSRTQNPTRMAYERCIADLESGTRGFAFASGMAATATVLELLDHGAHVVAS
                     DDLYGGTYRLFTRVRERSAGLAASYVDMRDRAALAAAIRPETRMIWVETPSNPMLKLI
                     DLSMVAEIGRERGILTVADNTFASPYVQRPLELGFDLVMHSATKYLNGHSDMVGGIVV
                     VGDNKELADRLAFLQNAVGAVAGPFDSFLALRGLKTLALRMERHCANAAAIADHLAHH
                     RRVARVHYPGLKSHPQHDLARRQMHGYGGMVTVLLDGGLAEARRFLERVEVFALAESL
                     GGVESLIEHPGIMTHASLPAAQRQALGIGDGLVRLSVGIEDVADLIADLDQALASA"
     gene            5546..6409
                     /gene="motA"
                     /locus_tag="VGP50_08310"
     CDS             5546..6409
                     /gene="motA"
                     /locus_tag="VGP50_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEV8027421.1"
                     /translation="MFLIVGSVVVLASVFGGYMAGGGHMVVLFQPFEYIIIFGAAIGA
                     YITSNRKPVLTGTLHAITGLLKSERFDKDAYLELLSLLYTLFKLAKSKGALALESHCE
                     NPHESTLFERFPQFAGDEHSVVFLCDYLRLLTLGTDNSHEMETLIDEDIATYHEEHVA
                     VASAVQTMADGLPALGIVAAVLGVIHTMGSITEPPAVLGHLIGGALVGTFMGVLLAYG
                     FVGPMASSIRGRGEAEQRYFLCIKAAILAYMQGYAPSVAVEFARKSLNTDVRPTFYEV
                     EDAVGALPAPT"
     gene            6418..7398
                     /locus_tag="VGP50_08315"
     CDS             6418..7398
                     /locus_tag="VGP50_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEV8027422.1"
                     /translation="MMAKQSNAPIIIKKVKKGGHDAPHGGAWKVAYADFVTAMMAFFL
                     LLWLLNVTTDIQKRGIADYFQPTIASKSQSGAGGVLGGVTIGQPGAQQAASVEPSLTL
                     SQPMLRQLNDSDDEDQDNAAGRDDVSDNGGAGPNRDRQRPKLSEAELERQVAEREDRR
                     FAAAEFALHQALEQMPDLKGLAENLIIDKTPEGLRIQLVDQDKSAMFASGSAEPDENA
                     RKLIAVVAQVVLRLPNKVSITGHTDAVSYMTNRKYSNWELSADRANASRREFEADGVP
                     PDRIDRVVGLAEHDLLVPDDPTSPRNRRISIVLLRSAKEKETASDPAAPR"
     gene            7463..>7737
                     /locus_tag="VGP50_08320"
     CDS             7463..>7737
                     /locus_tag="VGP50_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEV8027423.1"
                     /translation="MTEGTMPDSNGSPTPQTRARPIDSTPPLFAPPQHFYRTAALPCP
                     YVAGRIERKLITELTGQEATGLYNALSRAGFRRSHHLAYRPACAGCAA"
BASE COUNT         1258 a         2775 c         2473 g         1231 t
ORIGIN      
        1 ccgccgagct gtgccgaggc gctcgcccgc tcgctcgaga tggcgctcga cgatctgacc
       61 cggcgctacg gcgccgagat gaaggattgg cgctggggcc gcgcgcatga agccgcattc
      121 accaaccagc tgctgtcgcg cgtcccggtg ctgcgcggca ttttcgcgac gcggatcgcc
      181 gccgatggcg gcgatgacac gctcaatcgc gcggcgacgt tcacgtggga cgagaaagag
      241 cccttcgccg atgtccacgg cccaacgctg cgcatgatcg tcgatctctc ggacatcgcc
      301 gcgacgcgct tcatgatcgc gcccgggcag tccggaaatc cgctgtcgcc gcattatgcg
      361 gacctgatgg gacggtggcg cgaggtcggc tacctgacgc tggacggcgg cgccgagggc
      421 ggcacgctgg agctgacgcc cgcgccgtga ccgcgctccc ggtcactctc gccgacattc
      481 gcgccgctgc cgctgccatc gccggcaccg tgctgcgcac gccgtccgtc tcggcgccgg
      541 cgctcggcaa cctcctcggg gcggaggtcg tgctgaagct cgagacgctg catccgaccg
      601 gctccttcaa ggagcgcggc gccctctaca agctgctcag cctcacgccc gaggagcgcc
      661 gcgccggcgt cattgccatg tccgccggca accacgccca aggtctggct taccatgcgc
      721 gccggctcgg cattcccgtc accatcgtca tgcccgaggg cacgcccttc accaagatcg
      781 agcgcaccga aagctatggc gcgaccgtgg tgctgcgcgg cgccgggctc ggcgaggcaa
      841 agcaggcggc cgccgcgctc gcggccgccg gccacctcac gctcgtccac ccttacgacg
      901 acgcgcgcat catcgccggc caaggcacga tcgcgctcga gctcctcgcc gaccatccgg
      961 agctcgacac tctggtggtg ccggtcggcg gcggcggatt gatcgccggc atcgccattg
     1021 ccgccaaggc gctcaagccc gagatcacca tcatcggcgt cgagacaacg ctctttccct
     1081 cgatgcttca cgctctgggc cgctgcgccg cgccgccctg cgggcagacg ctcgccgaag
     1141 gcattgccgt gctggagccg gggcagttca cccgcccggt ggtggcggcc ctcgtggacg
     1201 agatcctgct cgccgacgaa acggcgctcg agcgcgcggt ggcgctgctg ctcgacgtcg
     1261 cgaagctggt ggtcgagggc gccggcgccg cgccgctcgc ggcgctcatg agcgagcgcg
     1321 agcgctgccg cggccggcgc atcggcctcg tcctctccgg cggcaacatc gatgcgcgcc
     1381 tcctcgcctc gatcctgcag cgcgggctgg tgcgcgccgg gcggctggtg cggctcagga
     1441 tcgagatcag cgacgtcccg ggcgcgctca gcaagatcgc cggcatcatc ggggagcagg
     1501 gcggcaacat cgtcgaggtg catcaccagc gcctcttcca cgacgtcccc gtgaagctcg
     1561 ccgaggtcga cgccgtcatc gagacgcgca accccatcca tgtgggcgaa ctcgtcgccg
     1621 ccctgatcgc cgccggtttc ccgacccgcc tcttgtccag cacggcgatg gacacgcgct
     1681 gaggagccct ccgatgctgc tctctcaccg accgtgccga tgatcgaaga ggcgcggcgc
     1741 acggccatcg tcgtcggagc gtcatcgggc attggcgaag cgctggcgcg acaattgacc
     1801 gcggagggat ggcgcgtgtg cctcatggct cgccgcctct cccggctcga agcgatcgcc
     1861 gagaccttga gccgcgacgc cctcgcgcgc catatcgatc tgagcgacgc ggaaaccgcg
     1921 gcggcgaccc tcacggccgt gatcgacgaa cttggtggcg ccgacctcgt cgtcatgagc
     1981 tcgggtaccg gctatcccaa tacggcgttg gactggccgc cggaccgcga aacgctggcg
     2041 gtcaacgtcg tcggttttgc cgcggtcgcg caggcggcca tgcggcattt catcgcgcgt
     2101 ggccgcggcc acctcgtggg catcacgtca atcgctgcct tgcgcggctt cgcgccgacc
     2161 gccgcctacg ccggctcgaa ggcgttacaa tcgctctatc tcgagtccct ccgggacctc
     2221 gcgcgtctgc gtcgcctgcc gatcgccgtg accgaggcac agccgggctt cgtcgccacg
     2281 gcgatgatgc aatcggagcg cgtgttctgg gtcgcttccg ccgccgtcgc cgcgcaacag
     2341 atcatgcggg cgatcaggcg ccgcaggaaa cacgcctaca tcacccggcg atgggcgatt
     2401 atcgcccttc ttctccgttg cctgccgcga cccggctaag cgcccggcgc gccgtcgacg
     2461 atgacgaggc gcgatttgcc ggcaatggcg cggatcgctc gcgcgtcgcg gtagtcgggc
     2521 gagtcgtgcc agcgctgtgc cgcttccatg gtggcgaact cgatgaccat gatgcgcttg
     2581 ggcgccgcgc cttcgagccc ggcgctgcgg ccgccccgcg ccagaagccg tccgccatgg
     2641 cgggccacgg aatcggcccc ggcggtctcg tagatcttgt acgcgtcgcg atcggtgacg
     2701 tcgatttctg caatcacata ggccgtcatg acttaagtcc ccgccaaaac acgcggcgaa
     2761 gatagcgcag gcatcgtcat ctgccattat ctaatgtata aaatcacaaa tgttcgccga
     2821 gcgcgcgagt cgccaggacg gccttgccgc tcaccgccgc cgggcgtagg taagggcagg
     2881 aggcgccatg atcgttcatc agaacgtgct ggactcgatc ggcaacacac cgctggtcga
     2941 gctgacgcgc ttcgacacgg gccgctgccg gctcttcgcc aagctcgaga accagaaccc
     3001 ggccggctcg atcaaggacc gcatcggcct ctccctcatc gaagcggccg agcgcgacgg
     3061 caaggtcgca ccgggcggca cgctcatcga agcgaccgcg ggcaataccg ggctcggcct
     3121 ggcgctggtc gcggcgcaga agggctatcg gctgatcctc gtcattccgg acaagatgag
     3181 tcaggagaaa gtgttgcatt tgaaggccct gggcgccgaa gttcgcatca cgcgctcgga
     3241 cgtcggcaag ggtcatcacg attattacca ggacatggcg gagcgcctcg ccgccgagat
     3301 gggtgcgttc tacgtcaacc agttcgccaa ccctgccaat cccctggccc atgagacgac
     3361 gacgggaccc gagatctggc ggcagatggg ccaccagctc gatgccgtcg tagtgggcgt
     3421 gggctcgggc ggcacgctca ccggccttgc ccgtttcttc gagcgggtcg ccccggccgc
     3481 ggagatggtt ctggcggatc ccgaaggctc gatcctggcc ccgctcgtcc gcaccggccg
     3541 caaggtcgag gccggctcgt ggctggtcga aggggtgggc gaggatttcg tgccaccgaa
     3601 ctgcgacttg agccgggtcc gcaccgccta cgccgtaagc gaccgcgaca gcttcgccgc
     3661 ggcccgcgac ttgctgcgcc gcgagggcat cctcggcggc tcctcgaccg ggactttgct
     3721 ggccgcggcg ttgcgctatt gccgcgaaca aacagaagca aagcgcgtgg tcacgctggt
     3781 ttgcgacagc ggcaacaagt acctgtcgaa aatgtacaac gatttctgga tgttcgatca
     3841 aggacttctc acgcgttctg aacaaggcga tttgaccgac ctgatcgcgc ggcgccatgc
     3901 cgaacgcagt gtcgtcaccg tgacccccga ggacacgctg atcacggttc atcgccggat
     3961 gaagctctac gaggtctcgc agctgcccgt gctcgagggc gagcgcctgg tcggcatcat
     4021 cgacgaatcg gatttgctgc tcgccgtcaa tgccgatccg gcggcgttcc gcgagccggt
     4081 gcgccgcgcc atggtgacgc gggtcgagac cgtggcgccc gacgcgccga tcgcggcgct
     4141 gctgccgatc ttcgccaagg accatgtcgc cgtgatcgag aaggacggca agttcctcgg
     4201 cctcatcacc cgcattgact tcatcaatca cttgcgccgg cgcgtccgct gacgaggaag
     4261 tttccgatga ccccgacaaa gagcaaccgc cgcggcttcg cgacgcgcgg catccatgcc
     4321 ggccagaagc cggacccgtc gaccggcgcc atcatgacgc cgatctacgc cacctcgacc
     4381 tatgtccagg agagcccggg cgtccacaag ggctacgagt actcgcgcac gcagaacccg
     4441 acgcgcatgg cctatgagcg ctgcatcgcc gatctcgaaa gcggcacgcg cggcttcgcc
     4501 ttcgcgtccg gcatggcggc gaccgcgacc gtgctcgagc tgctcgacca tggcgcccat
     4561 gtggtcgcct ccgacgatct ctatggcggc acctatcggc tcttcacgcg cgtgcgcgag
     4621 cgctcggccg gccttgccgc gagctatgtc gacatgcgcg accgcgcggc gctggcggcg
     4681 gcgatccgcc ccgagacgcg catgatctgg gtggagacgc cgtccaaccc catgctcaag
     4741 ctcatcgacc tttccatggt cgccgagatc ggccgcgagc gcggcattct cactgtcgcc
     4801 gacaacacct tcgccagccc ctatgtgcag cgcccgctcg aattgggttt cgacctcgtc
     4861 atgcattctg cgaccaagta tctcaacggc cattccgaca tggtgggcgg catcgtcgtg
     4921 gtaggcgaca acaaggagct cgccgaccgc ctggcctttc tccagaacgc cgtgggcgcg
     4981 gtcgccggac ccttcgacag cttcctcgcc ctccgcggcc tcaagacgct ggcgctgcgc
     5041 atggagcggc actgcgccaa cgccgcggcc atagccgacc acctcgccca ccatcgccgg
     5101 gtcgcgcggg tgcattatcc cggcctcaaa agccaccctc agcacgacct ggcgcgccgc
     5161 cagatgcacg gctatggcgg catggtgacg gtcctgctcg acggcggact cgccgaggcg
     5221 cggcgcttcc tcgagcgcgt cgaggtcttc gcgctcgccg agagcctggg cggcgtcgag
     5281 agcctcatcg agcatcccgg gatcatgacc catgcctcgc tgccggcggc gcagcgccag
     5341 gcgctcggca ttggcgacgg cctggtccgg ctttccgtcg gcatcgagga cgtcgcggac
     5401 ctcattgccg acctcgatca ggccctcgcg agcgcgtagc cctcgcgagc gctgcacgct
     5461 cccagggaaa acagagcacg atcggcaacc atatcttaac gcgactcccg cagcctagcg
     5521 ggctagtgaa ggcccgcacc cccgcatgtt cctcatcgtc ggctctgtcg tcgtcctggc
     5581 cagcgtcttc ggcggatata tggccggcgg cgggcacatg gtcgtgctct tccagccctt
     5641 cgagtacatc atcatcttcg gcgcagcgat cggcgcctac atcacctcga accgcaagcc
     5701 cgtactgacg ggaacgctgc acgccatcac cggtctcctc aagagcgagc ggttcgacaa
     5761 ggacgcctat ctcgagcttc tgagcctgct ctacaccctc ttcaagctgg ccaagagcaa
     5821 gggcgcgctc gccctcgaaa gccattgcga gaatccgcac gaaagcacgc tcttcgagcg
     5881 ctttcctcaa ttcgccggcg acgagcactc ggtggtgttc ctgtgcgact acctgcgtct
     5941 cctcaccctc ggcaccgaca actctcacga gatggagacg ctgatcgacg aggacatcgc
     6001 gacataccat gaggagcatg tggcggtggc gtcggccgtg cagaccatgg ccgacggcct
     6061 gcccgcgctc ggcatcgtcg ccgccgtgct gggcgtcatc cacaccatgg gctcgatcac
     6121 cgagccaccg gcggtactgg gccatctcat cggcggcgcg ctggtcggca ccttcatggg
     6181 cgtgctcctc gcctacggct tcgtcggccc catggcgtcg tcgatccgcg gacggggcga
     6241 agccgagcag cgctattttc tttgcatcaa ggcggcgatc ctcgcctaca tgcagggata
     6301 tgctccttcg gtcgccgtcg agttcgcgcg caaatcgctc aatacggacg tccggccgac
     6361 cttctacgag gtcgaggatg ccgtgggcgc cttgcccgcg ccaacctgat cggttgaatg
     6421 atggcgaagc agagcaacgc ccccatcatc atcaagaagg tcaagaaggg cggccatgac
     6481 gccccacatg gcggcgcgtg gaaggtcgcc tacgccgact tcgtcacggc gatgatggcc
     6541 ttcttcctat tgctgtggct gctcaacgtc accacggaca tccaaaagcg cggcatcgcc
     6601 gactactttc agccgaccat cgcgtcgaag tcgcaaagcg gcgccggcgg cgttctgggc
     6661 ggcgtgacaa tcggccagcc gggcgcccag caggccgcgt cggtcgagcc gtcgcttacg
     6721 ctgagccagc cgatgctgcg tcagctcaac gacagcgacg acgaggacca ggacaatgcc
     6781 gccggacgcg atgacgtctc cgacaatggc ggcgcgggac ccaatcgtga ccgccagcgc
     6841 ccgaagctca gcgaggccga gctcgagcgc caggtcgccg agcgcgagga ccgccgcttc
     6901 gccgccgccg agttcgcgct ccaccaggcg ctggagcaaa tgcccgacct caagggactc
     6961 gccgagaatc tgatcatcga taagacgccc gaaggcttgc gcatccagct cgtcgaccag
     7021 gacaagtcgg ccatgttcgc ctcgggctcg gccgagccgg acgagaacgc gcgcaagctc
     7081 attgccgtcg tggcccaggt ggtgctgcgc ctgcccaaca aggtctcgat caccggccac
     7141 accgacgcgg tctcctatat gaccaaccgc aaatacagca attgggagct gtcggcggac
     7201 cgcgccaatg caagccggcg cgaattcgag gccgacgggg tgccgcccga tcggatcgac
     7261 cgcgtggtcg gcctcgccga gcacgacctg ctggtgccgg acgacccgac ctcgccgcgc
     7321 aaccggcgga tcagtatcgt cctgctgcgc tcggcgaaag agaaagagac ggcttccgac
     7381 cccgccgcgc ctcgctaacc ctgttgcccc ctggcgcctg accggcgatc tcggtcataa
     7441 tcactcgaag agagggcgcc ccgtgaccga aggaacgatg cccgacagca atggctcgcc
     7501 gacgccgcag acgcgggcgc ggccgatcga cagcacaccc cctcttttcg cgccgccgca
     7561 gcatttctat cgcacggccg cgctcccctg cccttacgtg gccggccgca tcgagcgcaa
     7621 gctcatcacc gagctcaccg ggcaggaggc cacgggcttg tacaacgcgc tgtcgcgcgc
     7681 cggcttccgc cgcagccatc acttggccta tcgcccggcc tgcgccggct gcgccgc
//
LOCUS       DASYNS010000266         7614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_218672, whole genome shotgun sequence.
ACCESSION   DASYNS010000266 DASYNS010000000
VERSION     DASYNS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7614
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218672"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VGP50_08325"
     CDS             <1..469
                     /locus_tag="VGP50_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027424.1"
                     /translation="GVRTMGVNDFTFTDHTDVRPTILSLTGLTDDYAHDGRVVFEILE
                     DEAVPATLLANEGLLSVLASSYKAINAPVGPLALKTLQLSTAGLAGNDATFAQATAQI
                     ESITASRNAIASQMIAILEGAAFANQPVNQLQAIELIDQADALQFGTPVPSFP"
     gene            complement(636..2129)
                     /locus_tag="VGP50_08330"
     CDS             complement(636..2129)
                     /locus_tag="VGP50_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8027425.1"
                     /translation="MSLAEQYKAALESHLRGEGGEGALNTAYELGRKAYVDGLGILEM
                     AAVHHGALSRLVESGALPLDRPHMERASEFFAESISPFEMSFRVHLDTNAKLAALNET
                     LRLANDKLVAEIADRQRAEIALRQAQKLQAVGRLAGGVAHNFNNLLTVVLGNLDAARD
                     RAADDETLAQALATAARAADRGAKVTKQLLAFSREQMLRPEVIEPAKRLREIASLLGA
                     TLRGDIAIETEIGADLCTVEIDQLEFELAFLNLGFNARDAMPRGGLLRISAANRTMRD
                     ARLALDGDYLVISVADTGQGIRPEVLPRVFEPFFTTKGVGAGSGLGLSQVYGFANQSG
                     GAVDIESRVGGGTTVHLYLPARRAQPTAEGAVEHLQPHEHRAAGTVLVVDDDVDVANL
                     AAQLLTRCGFKVKLAYRAHAALDLLRKGEGVDLIFTDILMPDGMNGIQLAEEVKRRYP
                     ELPVLLATGYSDAAMDAGARGLQIIAKPYRARELCASVTALLGESDA"
     gene            complement(2164..2568)
                     /locus_tag="VGP50_08335"
     CDS             complement(2164..2568)
                     /locus_tag="VGP50_08335"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEV8027426.1"
                     /translation="MLAHETRVVIDKDSDVVTARQKGRDLALQAGLSGTTLMLVATAI
                     SEVARNIVVYAKCGEIVLGITQHAGRRGIMVIARDNGPGFPDIGGATRDGYSTGRSLG
                     LGLPGARRLMDDFDIVSEVGKGTTITMKKWGR"
     gene            complement(2555..2713)
                     /locus_tag="VGP50_08340"
     CDS             complement(2555..2713)
                     /locus_tag="VGP50_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027427.1"
                     /translation="MAVPILEQSAVLIASVQSAPTDADLMLLQQNLREEGLELLASDL
                     GEDGDAGA"
     gene            complement(2727..2852)
                     /locus_tag="VGP50_08345"
     CDS             complement(2727..2852)
                     /locus_tag="VGP50_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027428.1"
                     /translation="MAIRIAHSEFDAEDVETLKGLNEIACEVVRRKEPVGKRRPA"
     gene            complement(3008..3667)
                     /locus_tag="VGP50_08350"
     CDS             complement(3008..3667)
                     /locus_tag="VGP50_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027429.1"
                     /translation="MPAGPARERPFIFVLAGVNGAGKSSVGGGILSAHGFPWYNPDSF
                     ARELMVRNGSSPDEANADAWAYGKAQLEVAMAERTNFAFETTLGGTTIPRLLGEAART
                     HDIVMMFVGLASVDMHLGRVKLRVAQGGHDIPEERIRQRWISSRQNVIALLPRLAQLQ
                     VFDNSAEAGLGEDVPFPLLVLEMKDGRVLYPGRDDVQALEATPDWAKPIVAAAFRCQA
                     D"
     gene            complement(3667..4014)
                     /locus_tag="VGP50_08355"
     CDS             complement(3667..4014)
                     /locus_tag="VGP50_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027430.1"
                     /translation="MVPNIRQVEDLPRRNATEVKNKWGDLVREVRAHGSVAVTNHDKV
                     ELVVVEVGRYREMAALVEGAQGRQQAALAELAAEFDRRLASLKAPDARGRLDAAMASQ
                     GRVKPRPKAGSSF"
     gene            4409..6487
                     /locus_tag="VGP50_08360"
     CDS             4409..6487
                     /locus_tag="VGP50_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027431.1"
                     /translation="MLVARAEFARWQRRLLATGAALAALVGAPIGAPAATPDPGPTGV
                     GATSFLTLPFSSQVYQQSWGSSPTLLPGNLFWESGTFFTDPSQTAMAFQSANTIAQKG
                     IVPFGGAQLWEQTYILSQPVSNFYDPPSWVNAQYANQREFEAWVQWVQARPQLEDVAY
                     DDGTMPPYYRSFGGSWGHISPLTPLDAADWPPGVSNATFGDWYAYKWGQTAAQSGAYG
                     IELSDFSDSQPHFPTQWHDFNPRIVASFATYLGSAVPGSSTQTQYNWIAANAFNQWTD
                     FTAQGYAKFYAALAGQLAQNTGHDSLVIDQNDFWPGLLRLRGIDPRILLATVPTKNIV
                     FEWNTHTLEAGRGGAPAIYAMGQAVIGAAREPNARIGANLSSNDQDFWGGVAEYWSTL
                     SASDQQERGLKELKRLWLEAGWSMVADRSGSPRRALAYVQRDYFDTGSIDAAVSGLIQ
                     SIIPVTPFGPALYYPVSIERQVEKLIGAAGAQESAAYFNPNDLVTLKNAGAPVGYYVS
                     DAALNTLQPGAKPSAWVVLDRASPSDGSDLLPATELQALQAIAPVVGSASALASLPNQ
                     PLAYGAGLTGIGFYDQNGRLILTATNLGDASIDGTIALKTLASGIYAATDLFSDQVTT
                     FTVGQGQGFLPVSLSRWDTTAFAIEPMAAAPASAPASTWTPIPAPAPTPTPAWYNSQW
                     GNVYIYTYQP"
     gene            complement(6528..>7614)
                     /locus_tag="VGP50_08365"
     CDS             complement(6528..>7614)
                     /locus_tag="VGP50_08365"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEV8027432.1"
                     /translation="RLSLSARAIALAALVILLLSPESATGPSFQMSFAAVAGLIAFYE
                     ALRGRLAAWHTHAGLVRRVGLYVLGIAFTTMVTTVATMPFTIYHFNRFPLYSVTANAL
                     AVPITGFWIMPWALVACLLMPFGAEALALTPMGWGIDAVTAIAQHVTAWPGAVLAVPS
                     MPPGGLVLLALGGLWLCIWRRRWRWLGLAPAAAGAMTLLFVRPPDIIVAEDSRLVAVR
                     AADGSYLLSSARAEKFVEETWTRRAAAEAGAAWPVYGASADGRLSCGALACLYRAAGQ
                     RVALIRDGAALAADCGEADLVVSPMPAHRRCPAPLVIDRDDTGTKGSHAIWLDRHGIR
                     IETVSDWRGTRAWTPVSAPLSRERRLN"
BASE COUNT         1276 a         2605 c         2457 g         1276 t
ORIGIN      
        1 gggcgtgcgc acgatgggcg tcaacgactt tacctttacc gatcatacgg acgtccgccc
       61 gaccatcctg agcctcaccg ggctgaccga cgactacgcc catgacggcc gcgtcgtgtt
      121 cgagatcctc gaagacgaag cggtgccggc caccctccta gcgaatgaag gcctgctctc
      181 ggtcctcgcc agctcttaca aggcgatcaa tgcaccggtc ggccccctcg cgctcaagac
      241 gctgcagctc tcgaccgccg gcctcgccgg caatgacgcg accttcgccc aggcgacggc
      301 gcagatcgag agcatcacgg cgagccgcaa tgcgatcgcc tcgcagatga tcgccatcct
      361 cgaaggcgcg gctttcgcca accagccggt caatcagctc caggcgatcg agctgatcga
      421 tcaggccgac gcactgcagt tcgggacccc cgtcccatcg ttcccataac ctgccccctg
      481 actgaagcgc ccgcccgggt ccccgcccgg gcgggctttt tttgcgcccg ccccgcgcgc
      541 ttcggggcct cctcagcttg ttcaacatga tcggcgatgt cgacgcgcgc ctcgacgtga
      601 acactgatac cgcgcgccag cgcggggggc ggcgcctacg cgtcggactc gcccagcagc
      661 gccgtgacgg aggcacagag ctccctggcg cgatagggtt tggcgatgat ctgcaggccg
      721 cgcgcgccgg catccatcgc ggcgtcgctg tagcccgtcg ccagcaggac cggcagctcc
      781 ggatagcggc gcttgacctc ctcggccagc tgaatgccgt tcatgccgtc gggcatcaat
      841 atatcggtga agatcaagtc cacgccctcc cccttgcgca ggagatcgag agcggcatgg
      901 gcgcggtagg caagcttgac cttgaagccg cagcgggtca gcagctgggc cgcgagattc
      961 gccacgtcca catcgtcatc gaccacgaga acggttccgg ccgcgcgatg ctcatgaggc
     1021 tgcagatgct cgaccgcccc ctccgcggtc ggctgcgcac gccgcgccgg caaatagaga
     1081 tgcaccgtcg tgcctccgcc gacccggctc tcgatgtcga ccgcgccgcc cgactggttg
     1141 gcaaagccat agacctggct caagccgaga ccgctgccgg caccgacacc cttggtggtg
     1201 aagaaaggtt cgaagactcg cggcagcact tccggccgaa tgccctggcc ggtgtcggcg
     1261 acggagatga cgagatagtc gccgtcgagg gcgagccgag cgtcacgcat cgttcggttc
     1321 gccgccgaga tgcgcagcag gccgcccctg ggcatggcgt cgcgcgcgtt gaagccgaga
     1381 ttgaggaagg cgagctcgaa ctcgagctgg tcgatctcga ccgtacagag atcggcgccg
     1441 atctcggtct cgatcgcgat gtcgccgcgc agcgtcgccc cgagcagcga ggcgatctcg
     1501 cgcagccgct tggcaggctc gatgacctcg ggccgcagca tctgctcgcg cgagaaggcg
     1561 aggagctgct tcgtgacctt tgcgccgcga tcggccgcgc gcgcggccgt cgcgagcgct
     1621 tgcgccagcg tctcatcgtc ggcggcccgg tcccgcgctg catcgaggtt gccgagcacg
     1681 accgtgagga gattgttgaa gttatgggcg acgccgccgg ccagtcgccc gaccgcctgc
     1741 agtttctgcg cttgccggag ggcgatctcg gcccgctggc ggtctgcgat ctccgcgacc
     1801 agcttgtcgt tggccagccg caaagtctcg ttcagcgcgg cgagcttcgc attggtatcg
     1861 agatgcacgc gaaaggacat ttcgaacggc gagatgctct cggcaaagaa ctcggaggct
     1921 ctctccatat gcgggcgatc gagcggcagc gctccgctct cgaccaagcg gctcagcgcg
     1981 ccgtgatgga ccgccgccat ctcgagtatc ccgaggccgt cgacataggc tttgcggcca
     2041 agctcatagg cggtgttgag tgcgccctcg ccgccctcgc cgcggaggtg gctctcgagc
     2101 gccgccttat attgctcggc gaggctcatt gcgtggcgcc gccgatgggc gcggacggcg
     2161 ccatcagcgt ccccatttct tcatggtgat ggtggtgccc ttccccacct cggaaacgat
     2221 gtcgaaatcg tccatgaggc gccgggcgcc gggcagcccg aggccgaggc tgcgcccggt
     2281 cgagtaaccg tcacgcgtcg cgcccccgat gtcggggaag ccgggaccgt tgtcgcgcgc
     2341 gatcaccatg atgccgcggc gccccgcgtg ctgcgtgatg ccgagcacga tctcgccaca
     2401 cttggcatag acgacgatgt tccgcgccac ctccgagatc gccgtcgcca ccagcatcag
     2461 cgtcgtgccg gagaggccgg cctgcagcgc gaggtcgcgg cccttctggc gagccgtcac
     2521 gacgtcgctg tccttgtcga tgacgacgcg cgtttcatgc gccagcatcg ccgtcctccc
     2581 cgagatccga tgccagcagc tcgaggcctt cctcgcggag attctgctgg agcagcatga
     2641 gatcggcatc ggtcggcgcc gattgaaccg aggcgatgag gacggcgctc tgctcgagga
     2701 ttggaaccgc catcggagcg cttccctcag gcggggcgtc gtttgccgac cggctccttc
     2761 cgtcgcacga cctcgcaggc gatttcgttc aggcccttga gcgtctcgac gtcctcggca
     2821 tcgaattcgc tatgcgcgat tctgatcgcc atgtcgtttc tcccccgcgc gcggcaaatg
     2881 ctggagccat ggaccagcgc caccgcaatc gccgatggaa tagtatcatg gatcttcccg
     2941 tttcgctgcg gagctttgcc gctcgcgcga ttggcaggat tgcgacattc gcgcgtctgc
     3001 gcgggcctca gtccgcctgg caccgaaaag cagcggcgac gatcggcttc gcccagtcgg
     3061 gcgtcgcctc gagcgcttgg acgtcgtcgc ggccgggata aagcacgcga ccgtccttca
     3121 tctcgaggac cagcagcgga aacggcacat cctctcccaa acccgcttcg gcgctgttgt
     3181 cgaatacttg cagctgcgcc aggcgcggca gcagcgcgat cacgttctgc cgcgagctga
     3241 tccagcgctg tctgattcgt tcttccggaa tgtcgtggcc gccctgggca acccgcagct
     3301 tgacgcggcc gagatgcata tcgaccgagg ccagcccgac gaacatcatc acgatgtcgt
     3361 gtgttcgtgc ggcctcgccg aggaggcgcg ggatggtggt gccgccgagc gtggtctcga
     3421 aggcgaagtt ggttcgctcg gccatggcca cttcgagctg cgctttgccg taggcccagg
     3481 catcggcgtt ggcttcgtcc gggctcgatc cgttcctcac catcagctcg cgcgcgaagg
     3541 agtcggggtt gtaccaggga aagccatggg cgctgaggat gccgccgccg acagagcttt
     3601 tgccggcgcc gttgacgccg gcgagcacga agatgaaggg acgttcccgg gccggcccgg
     3661 cgggcatcag aaggaggagc cggccttcgg acgcggcttg acccggccct gcgacgccat
     3721 ggccgcgtca agccggccgc gggcgtccgg cgccttcaag ctggccagcc ggcgatcgaa
     3781 ctccgccgca agctcggcca gggccgcttg ctggcgcccc tgcgcgccct cgaccagcgc
     3841 cgccatttcc cgataccggc cgacctccac caccacgagc tcgaccttgt cgtggttggt
     3901 gacggcgacg ctgccatggg cgcggacctc gcgcaccaga tcgccccact tgttcttgac
     3961 ctcggtcgca ttcctgcggg ggagatcttc gacctgccgg atgttcggga ccatggcagc
     4021 tcctttgaga ggatctgagg gtcagagtat cggccaaaat ggccggtttg tccacaaggg
     4081 ctatatatgc cattttagct acaacagacg gttaacgaac gccgtcccaa agcgaaaaat
     4141 ccttcattgg tccatcacga ccatcgacgg cggagaaagc gtagcgctct gcacacggac
     4201 gcatccgccg cgcgcctgcg accgcgtcac agcccgcttg ccacattgtt cacaccacgt
     4261 gattcgggtg cggtagcaac tcgttgacga aacttataga cgatacgtcg tctgcggtcc
     4321 tgcgcacgac cgccaaagag cttcccctca ttgcgatagg acagatgaaa tgccgcgagt
     4381 tcgcgaggca tcttcgaaca tttcgcccat gctggtggcc cgcgccgaat tcgcgcggtg
     4441 gcagcgaaga cttttggcga ccggcgccgc attggccgct cttgtcggcg ccccgatcgg
     4501 cgcgccggca gccacgcccg atcctggacc gacgggcgtc ggagcgacga gctttctgac
     4561 gctgccgttt tcaagtcagg tctatcagca gtcctggggc tcatcgccga cgctgctgcc
     4621 gggaaacctt ttctgggaat ccggcacctt ctttaccgat ccaagccaga cggcgatggc
     4681 ttttcagtcg gccaacacca tcgcgcagaa ggggatcgtt ccgttcgggg gcgctcagct
     4741 ctgggagcag acctacatcc tgtcgcagcc ggtgagcaac ttctacgacc cgccgtcatg
     4801 ggttaacgct caatacgcca atcaacgcga attcgaagca tgggtgcaat gggtgcaggc
     4861 gcgtccacag ctcgaggacg tcgcctatga cgacgggacg atgcctccct attaccggag
     4921 cttcggcggc tcttggggac acatcagccc gctcacgccg ctcgatgcgg ccgattggcc
     4981 gcccggcgtc agcaacgcca ccttcggcga ctggtacgca tacaagtggg gacaaacggc
     5041 ggcgcagagc ggcgcctatg gcatcgagct ttccgatttc agcgattcgc agccgcattt
     5101 cccgacccag tggcatgatt tcaatccgcg gatcgtcgcg tccttcgcga cctatctcgg
     5161 cagcgcggtt ccgggttcct cgacccagac tcagtacaac tggatcgccg ccaacgcgtt
     5221 caatcagtgg accgacttta cggcgcaggg ctatgcgaag ttctatgccg cgctggctgg
     5281 ccagctcgcg cagaacacgg gccatgattc tctcgtcatt gaccagaacg atttctggcc
     5341 cggcctcctc cgcttgcgcg gcatcgatcc gcggatcctg ctcgcgaccg tcccgaccaa
     5401 gaacatcgtt tttgaatgga atacgcacac cctcgaggcg gggcgcggcg gcgcgcccgc
     5461 gatctacgcg atgggtcagg cggtcatcgg cgcggcgcgc gagccgaatg ctcggatcgg
     5521 cgccaatctg agctcgaacg accaggattt ctggggcggc gttgcggagt actggagcac
     5581 tctgagcgct agcgatcagc aggagagagg cctcaaggag ctcaagcgcc tgtggctcga
     5641 ggctggatgg tcgatggtgg ccgaccgctc cggcagcccg cgtcgcgccc tcgcctatgt
     5701 tcagcgcgac tatttcgata ccggcagcat cgatgccgcc gtcagcggct tgattcagag
     5761 catcataccg gtgacacctt tcggtccggc cctttattat ccggtgtcca tcgagcgtca
     5821 ggtcgagaag ctcatcggtg cagcgggcgc ccaggaaagc gccgcctact tcaatccgaa
     5881 tgatctcgtg acgctcaaga acgccggcgc tcccgtcggc tattacgtca gcgacgcggc
     5941 gttgaacact cttcagccag gcgccaaacc ttcggcatgg gtcgtcctcg atcgcgcgag
     6001 tccgagcgac ggctcggacc ttctgccggc aaccgagctc caggccctgc aggcgatcgc
     6061 tcccgtggtg ggttcggcga gcgctctggc gtctttgccc aaccagcctc tcgcttatgg
     6121 cgcgggactg accggcatcg gcttctacga tcagaacggg cggctcatct tgacggccac
     6181 caacttgggc gacgcgtcga tcgacggcac gatcgccttg aagacgctcg ccagcggcat
     6241 ctacgcggcg acggatctgt tttcagacca ggtgacgacc ttcaccgtcg gtcagggcca
     6301 gggctttctc cccgtctcgc tctcgcgctg ggacacgacg gcctttgcga ttgagccgat
     6361 ggcggcggca cccgcatcgg ctccggcttc gacatggaca ccgataccgg ctccggcgcc
     6421 gacgccgaca ccggcctggt acaattccca atgggggaac gtctatatct atacctatca
     6481 gccctagccc gtatcccgat aggtgagatc acccggggta ggtgagatca attgaggcgt
     6541 cgttcccgcg aaagcggcgc cgacacgggc gtccacgcgc gcgtaccgcg ccagtcgctc
     6601 accgtctcga tgcggatgcc gtggcggtcg agccagatcg cgtggctgcc tttcgtcccg
     6661 gtgtcgtcgc ggtcgatgac cagcggcgcc gggcagcggc gatgcgccgg cattgggctc
     6721 acgaccaggt cggcctcgcc gcaatcggcg gcgagcgcgg cgccgtcgcg gatcagcgcc
     6781 acccgctggc ctgccgcgcg gtagagacag gcaagcgcgc cgcaggagag gcggccatcc
     6841 gcgctcgcgc catagacggg ccaggccgcg cccgcctcgg cggccgcgcg ccgcgtccag
     6901 gtctcctcga cgaatttctc ggcgcgcgcg ctcgacagca gatagctgcc gtcggcggcg
     6961 cgcaccgcga cgagccgcga atcctccgcg acgatgatgt cgggcgggcg tacgaacagc
     7021 agcgtcatgg cgcccgcggc ggccggcgcg agccccagcc accgccagcg ccggcgccag
     7081 atgcacagcc acaagccgcc aagggcgagg agcacgagac cgcccggcgg catgctcggc
     7141 accgccagca cggcgcctgg ccaggccgtc acatgctgcg cgatcgcggt cacggcatcg
     7201 atgccccagc ccatgggcgt gagcgccagc gcctcagccc cgaacggcat cagcaggcag
     7261 gcgacgagcg cccagggcat gatccaaaaa cccgtgatcg gcacggcgag cgcgttggcg
     7321 gtcaccgagt agagcgggaa gcgattgaag tgataaatcg tgaacggcat cgtcgcgacc
     7381 gtcgtcacca tcgtcgtgaa cgcgatgccc aggacataaa ggccgacgcg gcggacgaga
     7441 ccggcatggg tgtgccaggc ggcgagccgc ccgcgcagcg cctcgtagaa ggcgatgagg
     7501 ccggcgaccg cggcgaagga catctggaag ctgggccccg tggcgctttc cggcgaaagc
     7561 agcaggatca cgagcgccgc cagcgcgatc gcgcgcgccg agaggctcaa gcgg
//
LOCUS       DASYNS010000267         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_62800, whole genome shotgun sequence.
ACCESSION   DASYNS010000267 DASYNS010000000
VERSION     DASYNS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62800"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..403
                     /locus_tag="VGP50_08370"
                     /pseudo
     CDS             6..403
                     /locus_tag="VGP50_08370"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
     gene            complement(410..1081)
                     /locus_tag="VGP50_08375"
     CDS             complement(410..1081)
                     /locus_tag="VGP50_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEV8027433.1"
                     /translation="MIIVHHLNNSRSQRILWLLEELGVPYEIKRYERDAATMLAPAAL
                     RAIHPLGKSPVITDGNQTIAESGAIVEYLVERHGGGRLAPPAGTPERLRYIYWLHYAE
                     GSAMPPLLMKLVFQRIAQGAAPALLRPLIRRIAQRTQASFVDPQLKLHLDYLEGELGK
                     SEWFAGNSFTAADIQMSFPLEAAAARGGLDASRPKLMAFLERIHARPAYKRALERGGA
                     YALLR"
     gene            complement(1170..2558)
                     /locus_tag="VGP50_08380"
     CDS             complement(1170..2558)
                     /locus_tag="VGP50_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8027434.1"
                     /translation="MKAIGWRLKRSYRAGAVALGVLTASYGSIEAATPQPDALALARL
                     LKDSQFATLEATIVGYQKRFEADHQTEWVIVAASDAFANSDPELEPRLNEWVAAMPES
                     YAAPLARGVFYDHLAWLSRGSGYAGRTDQRRFAEMERFFALAQPDLKRALDRNPNLIA
                     AYETLAEIAKATGDGEDAKRILNAGLAIAPESGTLHAEFLGGLQPKWGGQPGEIRAYL
                     ADVAERHPSVAALGWFKSYLDIEAGDQLFEEKKYEEALAQYDRVLQSAPQNPSVMSSR
                     AAVLTSLGRYDEAVAALEQARAGSPAWAGYHFQMAKIHYLQKHGPEALASLDAAIAFD
                     RLNPNYLYWRALTLQALGRFKEAARDLDDAETYGAFDISVQIERSHALSRFSARLDDA
                     IAAGRRATELRPESSLAWINYAAALAVKQDCKAKEALQTFHAVCEKEGQCQLEGTLSA
                     PSIIRYMHCDRT"
     gene            complement(2813..>3022)
                     /locus_tag="VGP50_08385"
     CDS             complement(2813..>3022)
                     /locus_tag="VGP50_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV8027435.1"
                     /translation="ATLILHGAQDWVVHVAAAYQLDAFLNTIGATHETHIYPDAGHGF
                     RDTVGADAVRRTIAFLDRHVGERAG"
BASE COUNT          514 a          957 c         1037 g          514 t
ORIGIN      
        1 cgctcgtgtt tgccgcctgg gcgtcgcccg aggctaagtc gcgctggttc cacggccccc
       61 gtggcgaatg gacggagaac ttgcgcgagc tcgattttcg cgtcggcggg cgcgaacgcc
      121 tgctcggcac gcgcatgagc acgggccacg tccacgcttt cgatgcccgc tattacgcat
      181 cgtgcccgat gagcgcatcg tctacgccta cgagatgcac atggacgacg tcaaaatatc
      241 ggtctctctg gcgacggtgg agttcaagcc cgacggtgcc ggtacgcgga tgatcttcac
      301 cgagcagggc gtcttccttg acagcttcga ggatggcggc ggccgcgagc aggggacccg
      361 cgtgctgctc gatcagctcg gcgcagcgct gagccagcgc tgaggcgcct cagcgcaaca
      421 gcgcgtaggc gccgcctcgt tcgagcgcgc gcttgtaggc gggccgcgcg tggatgcgct
      481 cgaggaaggc catgagcttg gggcggctcg cgtcgaggcc gcccctcgcc gcggcagcct
      541 cgagcgggaa gctcatctgg atgtcggcgg cggtgaaact gttgccggca aaccattcgc
      601 tcttgccgag ctccccctcg aggtagtcga gatggagctt gagctgggga tcgacgaagc
      661 tcgcttgcgt gcgctgcgcg atgcggcgga tgagcggccg cagcagcgcc ggcgccgccc
      721 cttgcgcgat gcgctggaac accagcttca tgagcagcgg cggcatggcc gatccctcgg
      781 cgtaatggag ccagtagatg tagcgcagcc gctcgggcgt tccggcgggc ggcgcgaggc
      841 ggccgccgcc atgacgctcg accagatact cgacgatggc gccggattcg gcgatcgtct
      901 ggttgccgtc ggtgatgacc ggggacttgc cgagcggatg gatcgcgcgg agcgctgccg
      961 gcgccagcat ggtcgcggca tcgcgctcat accgcttgat ctcgtaaggc acgcccagct
     1021 cctcgagcag ccagagaatg cgctgcgagc gcgaattgtt gagatgatga acaatgatca
     1081 tcaccattcc ccctgcgttt gcgcgcgcat catagggcaa atcagcggtg cagaaagacc
     1141 gagcgagaaa atccctcgca ccacctcggc tatgtcctgt cgcaatgcat gtagcggatg
     1201 atcgacggcg cggagagcgt cccttccagc tgacactgac cctccttctc gcagaccgcg
     1261 tggaaggtct gcagcgcttc cttggccttg caatcctgct tgacggcaag cgctgccgca
     1321 tagttgatcc acgcgagaga gctttcgggc cggagctcgg tggcgcggcg gccggcggcg
     1381 atcgcatcgt cgaggcgagc cgagaaacgg gacaaggcgt ggctgcgctc gatctggacc
     1441 gatatgtcga aggcgccata ggtctcggca tcgtcgaggt cgcgggcggc ctctttgaag
     1501 cgtccgagcg cctgaagggt gagcgcgcgc cagtagagat aattcggatt gagccggtcg
     1561 aacgcgatcg ccgcgtcgag gctcgcgagc gcttccggcc cgtgcttctg cagatagtga
     1621 atcttggcca tctggaaatg gtaaccggcc caggccggtg accccgcgcg cgcctgctcg
     1681 agcgccgcga cggcctcatc gtagcggccg agagaggtca gaacggcagc tcgggacgac
     1741 attacgctgg ggttctgcgg tgccgactga agcactcgat cgtattgcgc gagtgcctct
     1801 tcatatttct tttcctcgaa gagctggtcg cccgcttcga tatcgaggta gctcttgaac
     1861 cagccgaggg cggcgacgct gggatggcgt tctgcgacgt cggcaaggta ggcgcggatc
     1921 tcgcccggct ggccgcccca tttcggctga aggccgccaa ggaactccgc gtgcagcgtg
     1981 ccggactccg gcgcgatggc gaggccggcg ttgaggatac gcttcgcgtc ctcaccgtcc
     2041 cccgtggcct tggcgatctc ggcgagcgtc tcataggcgg cgatcagatt cgggttgcgg
     2101 tcgagcgcgc gcttcaggtc gggttgcgcc agcgcaaaaa agcgctccat ctctgcgaag
     2161 cgccgctgat cggtccgccc ggcatagccg ctgccgcggc tcagccaggc gagatgatcg
     2221 taaaagacgc cgcgcgccag cggcgcggca taggactccg gcatcgcggc cacccattcg
     2281 ttcagccgcg gctcgagttc cggatccgaa ttggcgaagg cgtcagacgc cgcgacgatc
     2341 acccattctg tctggtgatc cgcctcgaag cgcttctgat agcccacgat cgtcgcctcg
     2401 agggtggcga actggctatc tttgagcagc cgggcaaggg ccagcgcgtc cggctgaggc
     2461 gtcgccgcct cgatcgatcc gtacgacgcg gtcaggacgc cgagcgccac ggcaccggcg
     2521 cgataggacc gtttcaatcg ccaaccgatc gctttcatct gcttgccccc gcaatttgcg
     2581 tcactgaaag acgccggagg catcacagca gaaagcgatt gcggctcgat taagtcgtcc
     2641 ggccgcgcat tctctgaata ttcctaaaaa gcaagcgagt tcagcccgat gaacgggcgg
     2701 aaagagcgag cgcgagaaag ccctcgcgcg catcgcggga gacggaaagg gtcagtgcgc
     2761 agcgctggga ggagagaggt cgtctaaagc gcggcttgcc ggcgccgtgc cctcacccgg
     2821 cgcgctcgcc gacgtgccgg tcgagaaatg cgatcgtgcg gcgcaccgcg tcggcgccca
     2881 cggtgtcgcg gaagccgtgg cccgcatcgg gataaatgtg cgtctcgtgc gtcgcaccga
     2941 tcgtgttgag aaaggcgtcg agctgatagg ccgcggcgac gtgcacgacc cagtcctggg
     3001 cgccatggag gatcagcgtc gc
//
LOCUS       DASYNS010000268         3768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4433120, whole genome shotgun sequence.
ACCESSION   DASYNS010000268 DASYNS010000000
VERSION     DASYNS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4433120"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1687)
                     /locus_tag="VGP50_08390"
     CDS             complement(113..1687)
                     /locus_tag="VGP50_08390"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HEV8027436.1"
                     /translation="MALEDHSDLGSSAAPDLELKLAVTPEQMRRLRHHPLVASAHPSR
                     RSLARTYFDTPDLALHRRSLDLRVGKEGHRYVQTLTGSNGKASIPPTALAWQSPVPGA
                     APDLSRLAHYHAVSDIDASQLQPVFVSRIRRSTRVVHPDDATTVEVSCDEGHIETAGG
                     LTLPVCELELGLRAGPRQALYELARALNEAAPLRIETRSKADRGYALLAGDEHRHPRE
                     RGFGKVELTPELPAEEALAAILRHALWHMLANDQAALHGEAEGVHQMRVALRRLRATF
                     SLFRPMIPGEQRLWATGEMKWIATALGPARNWDVFAEHVEPLEQHFPEARGIAVLSRA
                     AQESRQAAYGLVQQTITAPRYTDFVLRMLTWTETRGWRQQSVSETSVHLLAPLGNLAA
                     ELLERRHRKARRLARRFGELAAAERHELRIAFKKLRYAADSLQELYRAKAVKRYVRRL
                     AVLQDDLGIVNDVATMDGLLEQLKETAGPGDGLRHGAALMQGWYGHVMAACEARLAKK
                     LDRFMDMRLFWRGAGS"
     gene            1865..3475
                     /locus_tag="VGP50_08395"
     CDS             1865..3475
                     /locus_tag="VGP50_08395"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEV8027437.1"
                     /translation="MARRRNRGNAPSLALRRRSVLSGMAGALLVPRAFAQSPAAPAIV
                     PSEKGRPLLPCGVASGDVTAHRAIIWSKTDRPARMVVELADDESFRRARRITGPTALE
                     DSDFTARLDVDGLTPGETVFYRVSFQDLADLGSVSAPLTGRFRAAPDRAQDISFAFSG
                     DEAGQGWGINPEIGGYRIYESMRRAKPDFFVHSGDQIYADNPIVAEAKTDDGRVYKNL
                     TVPAKAQVAQSLADYRGNFAYNFLDENKRRFNAEVPLLIQWDDHETRNNWYPGQILGD
                     ERYTQRSASLLSAWARRALFENNPMRFDRDDPERIYRSFSFGPALEVFMLDERSYRGP
                     NSPNRQMVLDADSAFVGAAQLTWLQRALERSKATWKLIASDMPLSLVVPDLNPDVPKG
                     TFEAWANGDNGAPAGRELEVARLLSFIKRKAIKNVVWVTADVHYAAAYRYAPEKARFT
                     DFAPFWEFVAGPINAGTFGPNALDMTFGPEEKFLSIPRDMKQNRSPLDGRQYFGLGRI
                     DGHSRALVVTLHDIDGKELYKVELTPEA"
BASE COUNT          606 a         1315 c         1249 g          598 t
ORIGIN      
        1 gctcccgaaa tcgcgacagc cgcttgcaaa agcgcacatc gcaaggtgag gaaaagccgt
       61 cgatcgcacg ccgagccgtc ctcatcctga ccgcaacggc gccgatccag tcctagctac
      121 ccgctccgcg ccagaacagc ctcatatcca tgaagcggtc gagcttcttg gcgagcctcg
      181 cttcgcaggc cgccatgaca tgcccgtacc agccctgcat cagcgccgct ccgtggcgca
      241 gcccgtcgcc gggtcccgcc gtttccttga gttgttccaa taatccgtcc atggtcgcga
      301 catcgttgac gattccgaga tcgtcctgga gcacggcgag gcgccggacg tagcgtttca
      361 cggctttcgc ccggtagagc tcctggagcg aatcggcggc gtagcggagc ttcttgaagg
      421 cgatgcgcaa ctcgtggcgc tctgcggccg ccagctcgcc aaagcgcctg gccagccggc
      481 gcgccttgcg atgccgccgc tccagcagct cggccgcgag attgccgagg ggcgcgagca
      541 gatggaccga ggtctccgac accgattgct ggcgccagcc gcgggtctcg gtccaggtca
      601 gcatgcgcag cacgaaatcg gtatagcgcg gcgccgtgat ggtctgctga acgagcccgt
      661 aggctgcctg ccggctctcc tgggctgcgc gcgacaagac ggcgatgccg cgtgcctcgg
      721 ggaaatgctg ctcgagcggc tccacgtgct cggcgaagac gtcccagttg cgtgccggac
      781 cgagcgccgt cgcgatccat ttcatctcgc ccgtcgccca gaggcgctgt tccccgggaa
      841 tcatcggccg gaacaggctg aaagtggcgc gcaggcggcg gagcgcgacg cgcatctggt
      901 gcacgccctc ggcctccccg tggagcgccg cctggtcatt ggcgagcatg tgccacagcg
      961 catggcgcag gatggcggcg agcgcctctt cggccggcag ctcaggggtt agctcgacct
     1021 tgccgaagcc gcgctcgcgc ggatggcggt gctcgtcacc ggcgagcagc gcgtagccgc
     1081 ggtccgcctt gctgcgggtt tcgatgcgca gcggcgccgc ttcgttgagc gcgcgcgcca
     1141 gctcgtaaag cgcttgccgc ggcccggccc tgagcccgag ctccaattcg cagaccggca
     1201 gggtgaggcc tcccgccgtc tcgatatggc cttcatcgca ggagacctcg accgtcgtcg
     1261 cgtcgtccgg atggaccacg cgcgtgctgc ggcggatgcg gctgacgaag accggctgga
     1321 gctggcttgc gtcgatgtcg ctcacggcat ggtaatgcgc cagccgcgag agatcgggcg
     1381 ccgcgcccgg caccggcgat tgccacgcca acgccgtcgg cggaatgctc gccttgccgt
     1441 tgcttccggt cagcgtctgg acgtagcggt ggccctcctt gccgacgcgc aagtccaatg
     1501 accgccgatg cagcgcgagg tcgggcgtgt cgaaataggt ccgggcaagc gagcggcggc
     1561 tcggatgagc cgacgccacg agcggatggt ggcgcaggcg ccgcatctgc tcgggcgtga
     1621 cggcgagctt gagttcgaga tccggagctg cgctgctgcc gaggtcggag tgatcctcga
     1681 gtgccatcgg ctgagcatat cccagcacgg cggcagcgca atgtcgggcc tcgtcgacac
     1741 gttcggaggg tagcgggggc ggccccgtgt cgccggtcgc tcgatgggat cccggcctgc
     1801 aatgctgtcg cgatgaggtc accatggcgt gctataccgg tgccggcccg agaggagaga
     1861 cgccatggcg cgccgccgga accggggcaa tgccccatcg ctcgcccttc gacgccgctc
     1921 cgtcctgtcc gggatggcgg gcgcgttgct cgtgccgcgg gccttcgccc agagcccggc
     1981 cgcgccggcc atcgttccgt ccgagaaggg gcggccgctc ctcccctgcg gcgtcgcgag
     2041 cggcgacgtg acggcacacc gcgccatcat ctggagcaag accgaccggc ccgcgcggat
     2101 ggtggtggag ctggccgacg acgaaagctt ccgccgtgcg cggcgcatca ccggtccgac
     2161 cgctctcgag gacagcgact tcaccgcgcg gctcgatgtc gacgggctca cgccgggcga
     2221 gaccgtgttc taccgcgtca gcttccagga cctcgccgat ctcggcagcg tcagcgcgcc
     2281 gctgacgggg cggtttcgcg ccgccccgga ccgggcgcaa gacatcagct tcgccttctc
     2341 gggcgatgag gccgggcagg gctggggcat caaccccgag atcggcggct accgcatcta
     2401 cgaatccatg cgccgcgcga agcccgattt cttcgtacac tcgggcgatc agatctacgc
     2461 cgacaacccg atcgtcgccg aggccaagac cgatgatggc cgcgtctaca agaatctgac
     2521 cgtccccgcc aaggcgcagg tcgcacagag ccttgccgat tatcgcggca acttcgccta
     2581 caacttcctc gacgagaaca agcgccgctt caacgccgag gtgccgctcc tcatccaatg
     2641 ggacgaccat gagacgcgca acaactggta tccgggccag attctgggcg acgagcgcta
     2701 cacgcagcgg agcgcatcgc tgctctccgc ctgggcccgg cgcgcgctct tcgaaaacaa
     2761 tccgatgcgt ttcgaccgcg acgatcccga gcggatctat cgcagcttct ctttcgggcc
     2821 cgcgctcgag gtcttcatgc tcgacgagcg cagctatcgg ggacccaact cgccgaaccg
     2881 ccagatggtg ctcgacgcgg actccgcctt cgtcggcgcc gcccagctca cctggctcca
     2941 gcgagcgttg gagcggtcga aggcgacctg gaagctcatc gccagcgaca tgcccttgag
     3001 cctcgtcgtt cccgatctca atcccgacgt acccaagggc acgttcgaag cctgggccaa
     3061 tggcgacaac ggagcgcccg ccggccgcga gctcgaggtc gcccgcctcc tgtccttcat
     3121 caagcgcaag gcgatcaaga acgtcgtctg ggtgaccgcc gacgtgcatt atgccgccgc
     3181 ttaccgctac gcgcccgaaa aggcgcgatt caccgacttc gcgccgttct gggaattcgt
     3241 cgccgggccg atcaatgccg gcactttcgg gcccaacgcg ctcgacatga cgttcgggcc
     3301 cgaagagaaa ttcctgagta ttccccgcga catgaagcag aatcgctcgc cgctcgacgg
     3361 gcgccagtat ttcggcctcg gccggatcga cgggcacagc cgcgccctcg tcgtgacgct
     3421 ccacgacatc gacggcaagg agctctacaa ggtcgagctc acgcccgaag cttagaggac
     3481 gctccgcgct tcagcgctgg accccggccg aacgcatgcg cgcatcgcaa aagcggcggc
     3541 tttgcgctaa agcagatctc gagcatgtgg aatcggtact tcccatctcg tcgtccccgc
     3601 gaaagcgggg atccaggggc taccagtacg tcgcttgccc tgggtccccg ctttcgcggg
     3661 gacgacgcaa cgggcagggt caagcgcgat ggcgacgagg attccgccgc ttcgggccgg
     3721 tgaatgtcag gtctggtggg cgcgccggaa ggccgcgtcg cgcccgct
//
LOCUS       DASYNS010000269         4355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2559204, whole genome shotgun sequence.
ACCESSION   DASYNS010000269 DASYNS010000000
VERSION     DASYNS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2559204"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..374
                     /locus_tag="VGP50_08400"
     CDS             252..374
                     /locus_tag="VGP50_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027438.1"
                     /translation="MPAGSPRELGRGYAALYQQEILQADYGCDFDFLRHTPLRR"
     gene            400..768
                     /locus_tag="VGP50_08405"
     CDS             400..768
                     /locus_tag="VGP50_08405"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV8027439.1"
                     /translation="MKIHNSNTVAAPGGPYRHGIETPPNARWLHIAGQIGVAPEDFDG
                     QADQCWRNIKAILAAAGMGVENLVKVTHFLTRAEDIAAYGRVRSRHLGEARPASTLLV
                     ISALARPGLLVEVEAIAAKA"
     gene            773..2233
                     /locus_tag="VGP50_08410"
     CDS             773..2233
                     /locus_tag="VGP50_08410"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HEV8027440.1"
                     /translation="MARYDLVLRGGSVLDGTGAAAVSADVALDGDRIAAIGTLAAGSG
                     REEVDVAGLTVAPGFIDVHTHDDRALLARPDMAPKASQGVATVITGNCGVSLAPLPAG
                     DLPPPLDLVANGESGRFRRFADYLDALEARPAAVNAACLVGHSALRVGAMDDLDRPAS
                     EGEISTMRRELTAALDAGAIGMSSGLWYAPAIAATKSELVSLARVLHDADALYTTHMR
                     DEADHVLDSLDESFAVGREANVPVVISHHKVLGKLNFGRSAETLPKIAAAITKQPLGL
                     DAYPYVASSTVLRKQNIGISLRILVTWSKAHPDVAGRELSAIAADWGVSQEEACDRLQ
                     PAGAIYFTMSEEDVRRILAFEHTMIGSDGLPHDTHPHPRLWGTFPRVLGHYSRELGLF
                     PLAEAVRRMTSLPAARFGLSRRGRLEPGAYADITVFDALTIIDRATFQVPAMPAAGIH
                     LVLVNGKTVWRDGMTIDNRPGRVLRRQAMRSEARSS"
     gene            complement(2237..2867)
                     /gene="bluB"
                     /locus_tag="VGP50_08415"
                     /pseudo
     CDS             complement(2237..2867)
                     /gene="bluB"
                     /locus_tag="VGP50_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
     gene            complement(2864..4009)
                     /locus_tag="VGP50_08420"
     CDS             complement(2864..4009)
                     /locus_tag="VGP50_08420"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEV8027441.1"
                     /translation="MTQALSPNAPLIGQAGSRYRLDTPALLIDLDALERNIAAMAVHA
                     RAVSVALRPHAKTHKSVRIARMQVDAGAIGICCATLGEAEVMVDAGIPGVHITSPQVT
                     PPKIARLIALNLRAHRGLSVVVDYPENLAALDRAALVAGNRLDVLVDFSAGHHRTGCA
                     TEADLAALASAAVAAEGLTFRGVQSYSGNLQHIPVRVERRAKALAQLDRLRGMIARLR
                     DGGLEVPIVTGAGTGTFDLDPEARVFTELQVGSYVFMDVDYLRAMREGRNAPPFETAL
                     FVATAVVSTNARDYVTTDGGLKCFATDGPQPEVASGAPPGSRYEFFGDEHGRLIVSEG
                     SPRPALGTIIECVTPHCDPTVNLHDWYHVVRGDTLVDVWPVDARGRR"
     gene            complement(4015..>4355)
                     /locus_tag="VGP50_08425"
     CDS             complement(4015..>4355)
                     /locus_tag="VGP50_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027442.1"
                     /translation="FVAHDLAVVENISHRIAVMYLGKIVELTDRQSLFSAPLHPYTEA
                     LLSAVPLPDPDAPRRRIILSGDVPSPINPPTGCRFHTRCPYAEARCRVEEPAMREVKP
                     GHLVACHLRG"
BASE COUNT          785 a         1426 c         1433 g          711 t
ORIGIN      
        1 gaatgcgcaa ggatagatca caccggcgtc gacataattg ccgccgcgcg agacggaacg
       61 cgaatgggga tttcggttca atgccgttct cgcactcccg gcacggaaaa ctcaagcgtc
      121 aatcttcacg agtttaaaat ggcgcgcgta gcgtgcgggc cttgtggttg tgtgccttac
      181 tcggcaatcg ttgtagatcg cgctggcacc atcagttgct atttgctgtc actcgattac
      241 ttggagagca tttgccggcg ggaagtccac gcgaacttgg ccgcggctac gccgcgctct
      301 atcaacaaga gatcctgcaa gcggattacg gctgcgactt cgatttcctg cgccacacgc
      361 cattgcggcg ctaacgatcc accgaggagg aaaaacacca tgaagattca caattccaat
      421 accgtcgccg cgcccggcgg accctatagg cacggcatcg agacgccacc aaacgctcgc
      481 tggctgcaca tcgccggcca gatcggcgtc gccccggagg atttcgacgg ccaggcggat
      541 caatgctggc gcaacatcaa ggcgatcctc gcggccgctg gcatgggcgt cgagaatctc
      601 gtgaaggtca cgcattttct gacgcgcgcg gaggacatcg ccgcctatgg cagggttcgc
      661 tcccgccatc tcggcgaggc acggccggcc tcgacactcc tcgtcatcag cgccctcgcg
      721 cggccggggc ttctcgtcga ggtcgaggcg atcgccgcca aggcttagtt ccgtggcgcg
      781 ctacgacctt gtgctgcgcg gcggcagtgt actcgacggc accggtgccg ccgccgtcag
      841 cgccgatgta gctctcgatg gcgaccgcat tgccgcgatc ggcacgctcg ccgcgggcag
      901 cgggcgggag gaggtcgatg tcgccgggct cacggtcgcg cccggcttca tcgatgttca
      961 cacgcatgac gaccgcgctc tgctcgcgcg tccggatatg gcgccgaagg cgagccaggg
     1021 agtcgccacg gtgatcaccg gcaattgcgg cgtgagcctc gcgccgcttc ccgccggcga
     1081 cttgccgccg ccgctggatc tcgtcgcgaa cggggagagc ggacgcttcc gccgtttcgc
     1141 tgattatctc gatgcgctcg aggctcggcc cgcggcggtg aatgccgcct gtctcgtcgg
     1201 ccattccgcg ctccgcgtcg gtgccatgga tgatctcgat cgccccgcga gtgagggcga
     1261 gatttcgacc atgcgtcggg aactcacggc cgccctcgat gcgggtgcca tcggcatgtc
     1321 gagtggtctc tggtatgcgc cggcgattgc ggcgacgaag agcgagcttg tctcgctcgc
     1381 gagagtcctg cacgacgccg acgcgctcta cacgacgcac atgcgcgatg aggccgacca
     1441 cgttctcgac agcctcgacg agagttttgc cgtggggcgc gaggccaacg taccggtagt
     1501 gatctcgcac cacaaggtgt tgggcaagct gaatttcggc cgcagcgccg agacattgcc
     1561 aaagatcgcg gctgccatca ccaaacaacc cctggggctc gacgcctatc cctacgtcgc
     1621 ctcgtcgacg gtgctgcgca agcagaacat aggcatatcg ctcaggatcc tcgtgacctg
     1681 gtcgaaagcc catcccgatg tcgccgggcg cgagctgtct gcgatcgcgg cggattgggg
     1741 cgtgagccag gaggaggcct gcgaccggct ccagccggcg ggcgccatct acttcaccat
     1801 gagcgaggaa gatgtccgcc gcatcctcgc attcgagcac acgatgatcg ggtcggatgg
     1861 attgccgcac gacacgcatc cacatccgcg gctgtggggc accttcccgc gcgtcctcgg
     1921 ccactacagc cgcgagctcg ggctctttcc gctggcggaa gccgtacggc gcatgacgag
     1981 cttgccggcc gcaaggtttg gcctcagccg gcgcggacgg ctggaacccg gcgcctacgc
     2041 cgacatcacc gtcttcgatg ccttgacgat catcgaccgc gccaccttcc aggtgccggc
     2101 gatgcccgcc gccggcattc atctcgtgct cgtgaacggc aagaccgtgt ggcgcgacgg
     2161 tatgacgatc gacaatcgcc cgggccgggt gctgcgccgc caagcgatgc ggagcgaggc
     2221 gcgatcgtcc tgagcctcat cgacgcagca cgaattcctc ccaaggccgc cgctgttccc
     2281 agccctcgcg cgcgagctcg ggtgtaaccg tctcgctctg gggtagccta agcaaaaata
     2341 tcccaagaag atccagtcgc cgggcacttc gaggatccgg ctcacggcaa ccggatcgag
     2401 aatcgagacc catcaggccc aagccttcag cgcgcgccac gagccaaagg gtgtgaaccg
     2461 ccatgacggc ggagtactcg gccatttccg gcatcgtgcg ccgccccagg ccgtgccctt
     2521 gcgcggtacc gcggtcggcg aagagcgcca aatgcacggg cgcctagtcg agaccggcga
     2581 gcttcagccg ggcgtagagc tgtgcgcgct cgccggcata ggaggagagt gccgcgtcat
     2641 tgcagcggca gaattccgct cgcactgctg ccgcgccgcg cgacaccgtc gacgatgaca
     2701 aagcgccagg gctggctcaa cccgaccgag ggtgcgagcg atgcgagtgc cagaagacga
     2761 tcgacggtgc ccgtcggcaa agcaccctcg cggaagcggc gcacatcgcg ccggcagatg
     2821 atcagctcgc gcagcttggt gcgaaaatcg ggatcgaaag cggtcaccgc ctccctcggg
     2881 catcgacggg ccaaacgtcc acgagcgtat cgccgcggac gacatggtac cagtcgtgaa
     2941 gattgacggt cggatcgcaa tgcggcgtca cgcattcgat gattgtgccg agcgctggac
     3001 gtggcgaccc ttccgatacg atcaaccggc catgctcgtc gccaaagaat tcatagcggc
     3061 ttccgggcgg cgcgcctgaa gcgacctccg gctgcgggcc gtcggtcgcg aagcatttaa
     3121 ggccgccatc ggtggtgacg tagtcgcgcg cattggtcga cacgaccgca gttgccacga
     3181 acagcgccgt ctcaaagggt ggcgcgttgc ggccctcgcg catggcgcgg agatagtcga
     3241 catccatgaa gacgtaggag ccgacctgca gctcggtgaa aacacgcgct tcgggatcga
     3301 ggtcgaaggt cccggtgccg gcacccgtga cgatcgggac ttcaagaccg ccgtcgcgca
     3361 agcgcgcgat catgccccgg agccgatcga gctgcgccaa cgccttggca cggcgctcga
     3421 cacgaacggg aatgtgctgg agattgccgg agtaagattg aacgccgcga aacgtcagcc
     3481 cttctgccgc caccgcggcg cttgccaacg ctgcgaggtc tgcctccgta gcgcaaccgg
     3541 tgcgatgatg tccggccgag aaatcgacca gaacatcgag ccggtttccc gccaccagcg
     3601 ccgctcggtc gagcgccgcc agattctcgg gatagtcgac gacgacactc aggccacgat
     3661 gggcccgaag gttcaacgcg atcagccgag cgatcttggg tggcgtcact tgcggcgagg
     3721 tgatgtggac gccgggaatg cccgcatcca ccatcacctc ggcctcgccc aaggtcgcgc
     3781 aacagatgcc gatcgcaccg gcgtccactt gcatccgagc gatgcggacg gatttgtgtg
     3841 tcttcgcgtg cgggcggagc gccacgctta cggcgcgcgc atgcaccgcc atcgccgcga
     3901 tgttgcgctc gagcgcgtcg agatcgatga gcagcgccgg cgtgtccaga cggtagcggc
     3961 tgccggcttg accgatgaga ggggcgttgg ggctcagagc ttgtgtcatg gctgctatcc
     4021 ccgcaaatgg caggcgacga gatgccccgg tttcacttcg cgcatcgccg gttcctcgac
     4081 gcggcagcgc gcctcggcgt aggggcagcg cgtgtggaag cgacagccag tgggcggatt
     4141 gatcgggctc ggcacatcac ccgagagaat gatacgccga cgcggcgcgt cgggatcggg
     4201 cagcggcacg gccgagagca gcgcctccgt gtagggatgg agcggcgccg agaagaggga
     4261 ctggcgatcg gtcagctcga cgatcttgcc cagatacatc acggcgatgc ggtgactgat
     4321 gttctcgacg accgcgagat catgcgcgac gaaga
//
LOCUS       DASYNS010000270        20213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2872157, whole genome shotgun sequence.
ACCESSION   DASYNS010000270 DASYNS010000000
VERSION     DASYNS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 20213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20213
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2872157"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VGP50_08430"
     CDS             complement(<1..572)
                     /locus_tag="VGP50_08430"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEV8027443.1"
                     /translation="MATSALPSIASEGNLARYLQEIRKFPMLAPEEEYMLAKRWREHA
                     DPDAAHRLVTSHLRLVAKIAMGYRGYGLPLSELISEGNVGMMQAVKRFDPDRGFRLAT
                     YAMWWIRAAIQEYILHSWSLVKMGTTAAQKKLFFNLRKLKGQLQAIEEGDLSPENVKR
                     IANELGVSEPDVVSMNRRLAAPDHSLNAPL"
     gene            complement(676..1653)
                     /locus_tag="VGP50_08435"
     CDS             complement(676..1653)
                     /locus_tag="VGP50_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV8027444.1"
                     /translation="MSESETTRRVTAEPADAGERLDRLLAARLDDLSRTRVKNLVEAG
                     RVTADGATVRDPSLRVKPGQSFVLTVPAAVDDTPVPQAMALTVVYEDPHLLVIDKPAG
                     LVVHPAPGNPDRTLVNALLAHCGASLAGIGGVKRPGIVHRLDKDTSGLMVVAKDELAQ
                     AKLAADFAARRITRAYQGVVWGVPEPRAGEIASNIGRSPRDRKKMAVLRQGGKPAVTR
                     YRVLRAFKDVAALVECRLATGRTHQIRVHLSAAGHPLIGDPVYGNPRSPGRLRALSLE
                     PRAQLAAFPRQALHAVLLGFRHPSTGENIEFSSKLPSDIKELVHLLDTV"
     gene            1839..2900
                     /locus_tag="VGP50_08440"
     CDS             1839..2900
                     /locus_tag="VGP50_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HEV8027445.1"
                     /translation="MNFRSDNEAGVSPQILAAIAAASHGTAPSYGEDEITARLARRFA
                     EIFERDVVVFPVATGTAANALALAALTPPWGVVFCHENSHVATDECGAPEFYTGGAKL
                     IGLAGGEGKLAPASVEPHLVGIDVPHHALPAAISLSEATEAGTVYSPEQVGALGAVAR
                     RHRLGLHMDGARFANAVAALGCAPADLTWRAGVDVLSFGATKNGALAAEAVIFFDPEK
                     AADFAFRRKRGGHLLSKMRFCSAQLDAYLADGLWLENARHANRMAARLGAGLARVPLA
                     RLRHPVEANEVFVELPEAVIQALAAAGFAFHRWRSAASSCLRLVTAFDTRAADVDAFI
                     AAMLASAPPDGSSEHKSTL"
     gene            3061..5061
                     /locus_tag="VGP50_08445"
     CDS             3061..5061
                     /locus_tag="VGP50_08445"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV8027446.1"
                     /translation="MAKRNGDGNGTAANGAPKPLPKKAAAKNGVARTLGPVSDLERHL
                     PTEWWRTLFNSLYLETDGDVVENDRNTEQEIDLLVRSAGLEPNDRILDLCCGQGRHAL
                     ELARRGFKNVMGIDRSRYLVRLARKRARQRGLPVSFHEGDARKFRVPESSVHCVCILG
                     NSFGYFDREEDDIAVLGAVKRALASGGTLVMDLTDGEWMRSHFEARSWEWIDQNHFVC
                     RERALAGDGERLVSREVVVHAERGVIADQFYAERLYSRERLQTLVESVGFANLRFHAF
                     LSPDSPRNQDLGMMAHRMFLTCEAPRKVERQRHEVIFPEVTVLLGDPRLPDPVKRNGQ
                     FNTEDLDTVARLKSALAEIPGYRFRFLDNHASLLADLRAQRPDFVLNLCDEGYNNDAF
                     MELHVPAVLELVDAPYSGAAPSCLGLCYNKSLVRAIAASLDVPVPLETYFSSDDQAAT
                     IPSVFPALIKPNFGDSSIGITQEAVVYSWEQAINYLSGLREQMPGRPILIQEFLTGAE
                     YSIGIIGNPGMALRALPPLEVDYSQLDRDLPQLLAYESKWIPDSPYWSQIKYREAQID
                     EDTRRRLVDHSNILFERLGCRDYARFDFRADAEGEIKLLEVNPNPGWCWDGKLNMMAT
                     YAGLRYSDMLKLILEAAQERIAAQRPQVVAAATPERLAVGQR"
     gene            5604..6764
                     /locus_tag="VGP50_08450"
     CDS             5604..6764
                     /locus_tag="VGP50_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEV8027447.1"
                     /translation="MKKKTDKKADRLRRIDRFLAERQPETPCLVVDLEVVADAYQALR
                     TYLPLARVFYAVKANPASEIVDLLHGLGSSFDVASRGEIELCLGRGVAAERISFGNTI
                     KKETDIAYAHQRGVRLFAFDSAAELEKLARAAPGARVFCRILVDGEGAEWPLSRKFGC
                     APEMAVELLRRAKAQGLDPYGVSFHVGSQQTNLEQWDGAVGRAARMFSLLAQTDINLR
                     MVNIGGGFPAHYRGDVPGVERYAAAVMAAITRHFGNDLPEIIIEPGRSMVGDAGIIQS
                     EVVLISQKAFGDEKRWVYLDVGKFNGLAETMDESIKYRIKTPADGGPTGPTVIAGPTC
                     DSADILYERTEYRLPMALKVGDKVQILSTGAYTSSYASVGFNGFSPIKTYCI"
     gene            complement(6752..7471)
                     /locus_tag="VGP50_08455"
     CDS             complement(6752..7471)
                     /locus_tag="VGP50_08455"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEV8027448.1"
                     /translation="MPVARPTAALMLASLLLAPLAARAATVDLALVFAVDVSESVDAE
                     EYELQHEGIARAFESQPLLDAIKGGAHGAIDVLVLEWSDPEKQVVTVDWMRVSDKASA
                     ADFTAKLRASKRSSSGLTAIGNALLAAQAAFRLLPDKAARQVVDVSGDGIANIGPPPQ
                     AVRDEMVAAGITINGLAILKTEPWLSSYYDQYVVGGPGGFLMQVEDFASFAQAMQQKL
                     LAEVAAARRERHAATFQSQMQ"
     gene            complement(7616..8686)
                     /locus_tag="VGP50_08460"
     CDS             complement(7616..8686)
                     /locus_tag="VGP50_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8027449.1"
                     /translation="MRFLAVAIVAALLAGCAAAIAPHDAPPPAPRSAAANAPELTGDR
                     FVAADGAALPLRRWLPPGSPAAVILALHGFNDYSNAFAGPGAAWARLGIATYAYDQRG
                     FGAAPGRGRWVGGQRLAEDAAGAARLLRQRYPSVPLYLLGESMGGAVAVLAASGKSGA
                     PVPAVDGVILVAPAVWGRETMTLLERVSLWFAGLMPDASFSPDIIPIEIMPSDNIAML
                     RAFNADPLVIVETRADAINGLVDLMSAALHAGAWFDAPALLLYGEHDEIVPRAPMQRF
                     VRALPPQAELRQRVALYPRGYHMLLRDLDASLPVGDVAAWVADHAAPLPSHADRDARA
                     RLTGRSEPLSAAGRSLDAGKTN"
     gene            complement(8791..10377)
                     /locus_tag="VGP50_08465"
     CDS             complement(8791..10377)
                     /locus_tag="VGP50_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV8027450.1"
                     /translation="MQTSPSKAPTTPLRHGIAGSAAGAAALLAILALASCGGAPPSGA
                     IANDTTRFYQEAYSGIAKYYIEPVQPGALAIAGLRNLTSIDATLSVEWSGSEVVAHHG
                     LSSTHFSAPDARDAEQWAELTETVLNWARERSPAVAGLSPDGLDATVVEGSMTILDRF
                     SHYAAPELARERRASRDGFGGIGVTLNAEGDGIRVVQIMPETPAAEAGLQVDDRILAI
                     DGVETTALTREEVVRRLRGPNESLIALAVTRAPSPKRLNFSMRRAHIVPPTVTLREEN
                     GIAHLRLSGFNQQTAPSVADLLQQAHRDMGASLRGLILDLRGNPGGLLDQSVEVASLF
                     LDATPVSSTIGRVPESIQYFAAPHRDVERLPMVVLVNGGSASASEIVAAALQDTGRAV
                     VVGTASFGKGTVQNVQHLSNDAEMTVTWARLLTPGGYVLHEHGVVPTVCTANLPEDGA
                     GLGGALRDESTGLVRPRASLDEAGWRRLRALCPGDREDHDVEVMAAKRVLDDPALYAR
                     ALGTSPAAALRPVATAGMVR"
     gene            complement(10444..12714)
                     /gene="rnr"
                     /locus_tag="VGP50_08470"
     CDS             complement(10444..12714)
                     /gene="rnr"
                     /locus_tag="VGP50_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV8027451.1"
                     /translation="MPRRAPAKRRKHGRRAKPPSNDQGEAAAKNKRPRAAGRLPTREE
                     LLEFIAASAIPVGKRDIVRAFRVPPADRIALKELVREIERAGSAERGRKRRLQPVGSP
                     PEIGVIEITDIDLDGEMHGKPVASGDAQNAPILFIVTDRGAPALGVGERAVARFVRAD
                     DGTYEAHVIRAIGAAPDRVLGVFRRDRDGGRIEPTDRKLRTEFRVAHVDTADAQDGEI
                     VLAEVLPARRLGLPQARVVQRVGDSADPRAFSLIAIHAHGIPTDFRPEALTLAEKARP
                     VDLGNRTDLRAIPLVTIDGADARDFDDAVWAEPDPERQSGWHILVAIADVAWYVRPGD
                     ALDHAARERGNSVYFPDRVVPMLPEALSNELCSLKPGVERACMAVHLWLDAEGKVHRH
                     RFLRGLMRSAARLTYEAVQDAVDGRPNDTAGSVLEVLRPLYGAYHALERARRTRGTLD
                     LDLPERRILLDPSGRVARIEPRQRLDSHKLIEEFMIAANVAAAQELERLRRPCMYRVH
                     DAPDPAKLSALREFLEGIGIPGLKLAKGQLVRPHHFNEILRRAAGTPYQSLVNELVLR
                     SQAQAIYSPENIGHFGLGLTRYAHFTSPIRRYADLLVHRALIAGLDAGAGGLPPTEPK
                     EFAASGDHISMTERRAAAAERSASDRYTAAFLAERVGASFPGRINGVTRAGLFVTLAE
                     SGADGLLPMRSLPDDYYNHDEARHRLVGRRFGRVYTLGDAVTVRLAEANTVTGSLLFA
                     LEETDRPPGRRSSTRR"
     gene            complement(12653..15334)
                     /gene="topA"
                     /locus_tag="VGP50_08475"
     CDS             complement(12653..15334)
                     /gene="topA"
                     /locus_tag="VGP50_08475"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV8027452.1"
                     /translation="MNVVIVESPAKAKTINKYLGKGYKVLASYGHVRDLPAKDGSVRP
                     DEGFAMSWEVQDRAEKRMSAIAKAVEGAEHLYLATDPDREGEAISWHVREVLKNRKAL
                     KGVDVKRVVFNEVTKSAVLEAFRHPRELNRELVDAYLARRALDYLVGFTLSPVLWRKL
                     PGSRSAGRVQSVALRLICERESEIEAFKPREYWGIEVEFQTPSGERFTARLTHIDGKR
                     LDKFDIADEATARAAAERILRQQSYAVATVEKKQIRRNPPAPFTTSTIQQEASRKLGF
                     GASRTMRIAQRLYEGVDIAGDTVGLITYMRTDGVTMSSEAITAARDLIEKQYGAAYRP
                     DEPRVYKTQAKNAQEAHEAIRPTDMFGRPEKVARDLEPEQLQLYELIWKRAMASQMSS
                     ALIDQVAVDIASADGQLQFRATGSTIVFDGFLTLYREDEDDTEQDDENRRLPPLNERD
                     KLGRGTVDPQQHFTQPPPRYTEASLVKKLEELGIGRPSTYASIIQVLQDRDYVRLDKK
                     RFIPEDRGRVVTAFLESYFRRYVEYNFTADLENQLDDISDGRIAWQKVLEDFWRDFSL
                     AVAGTKDLSITQVLDTLDEDLGPHFFPPAADGRDPRLCPGCNNGRLSLKLGKYGAFIG
                     CAHYPECRYTRALAIENANGNGQELTGPRELGVDPEINLAVTVRKGPYGYYVQRDAAS
                     EDEKPKRMGLPKGVSPTEIDLAHALKLLSLPREVGLHPETGKPIIAAIGMYGPYIKHE
                     KLYKSVRGDDDVLTIGLNRAVSLLAEPKKGGRRGPTPLRVIGNHPEDGAPVNLFDGKY
                     GRYVSHDGINATLPEETEPEAVTLEQALELLAARRKVKGGKKAKAKAKVVAAPAAKPN
                     KVAAASAAKPEKKGTSTRAKAGARKKTQARPTRKAAE"
     gene            complement(15384..16505)
                     /gene="dprA"
                     /locus_tag="VGP50_08480"
     CDS             complement(15384..16505)
                     /gene="dprA"
                     /locus_tag="VGP50_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEV8027453.1"
                     /translation="MSAAPRVLNPAEKLDWLRLIRSENVGPVTFYQLLARFGSAAAAL
                     DALPDIARRGGRDRPITVFTRGAAERELQSLDRLGARLVAWGEPDYPSALAAVEDAPP
                     LLSVLGQAALLKRPAIAVVGARNASTNGRRFARDLALQLGQKGLIVVSGFARGIDTAA
                     HEGALPTGTVAVLAGGIDILYPEENRALRDAIIERGALLAEMPVGTVPKGRHFPRRNR
                     IISGCSLGVVVVEGALRSGSLITARFALEQGREVFAVPGSPLDPRCRGTNDLIRRGAT
                     LTETAEDVTAQLQGPLSPRAPARRSAWSDAPPEGPAGGSELETARRRVLERLSPTPVG
                     IDDLVRECQLPQSLTMSVLLELELAGRLERQPGNLVALL"
     gene            17127..18476
                     /locus_tag="VGP50_08485"
     CDS             17127..18476
                     /locus_tag="VGP50_08485"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV8027454.1"
                     /translation="MTRTLIKSGMIVTLDRRIGDLAQGDVLIDGERIAAVAPAIAADD
                     AQVIDARDSIVMPGLVNAHIHTWQTGLRGVAGDWTILEYLHHMHASIAPHFKPDDVYV
                     ANLVGALNQLNSGVTTMVDWCHNNPTPAHSDAAIDGLKEAGLRAVFLHGSPKPDVKPG
                     QKHFSEIPHPRVEIERLARDRLPSRDGLVTLGMAVLGPAYSTYEVSRHDLALARALGM
                     LCSMHVGGGAMRTPDGFVRLAGEGLIDRRVNIVHGNNLAGDHLRALLDNGASVTVTPE
                     VELQMGFGDCLTGRLREAGRAPSLGSDVESSMGGDMFTTMRMALQAQRAIDNAKVIAR
                     SGGAPEHLTIGCREALEWATIEGARMAGLDDRIGSLTPGKQADIVLLRADDLNLCPVV
                     DPVRSIVLHAGAANVDTVIVAGSVLKRDGALLRNDLARRKAELVSSSRRILAAPVTL"
     gene            18500..19753
                     /locus_tag="VGP50_08490"
     CDS             18500..19753
                     /locus_tag="VGP50_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV8027455.1"
                     /translation="MRGNIWGLAGLLLVSIAIAAPPARAAEPLKIGFSMSLTGNLASG
                     GNSALVAMQIWAADANAKGGLLGRPVQLVYYDDQSSSANVPGIYAKLIEVDKVDLVVS
                     GYATNQIAPAIPIVKQKNMVFMALFGTGVNDRFNYDKYFQILPNGKETRFGPSLGFLE
                     TAVTMTPKPKTIALVGADAEYAQTVLSGARDKAKELGLTIVYDKSYPPATVDFVPILR
                     AIQATAPDVVYVASYPQDSVGMVRASHEVGLKTKMFGGGMIGLAFGPIKQQLGPLLNG
                     IVSYDVYVPEPTMKFPGIEAFLEHYREKAIAAGIDPLGFYLPPFAYAEMQILGQAVSD
                     VGSLDQAKIAEHIHATAFDTIVGRVKFAENGEWETSRILFVQYQGIQGNDVEQFKQAG
                     KQVILYPPELQSGKFLYPYTEFQQP"
     gene            complement(19757..>20213)
                     /locus_tag="VGP50_08495"
     CDS             complement(19757..>20213)
                     /locus_tag="VGP50_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV8027456.1"
                     /translation="LRTGSRVLAALTLLLDAGKGAAAVLIAGAFSPDMAALAAGAAFL
                     GHVFPVWLGFKGGKGVATAGGILLAYAWPVGLAALGTWLLVAVLTRYSSLAALAAAVL
                     APLYAWLITREIQPTEVVLFLAIVILLRHAANIRRLLKGEESTIRLGKS"
BASE COUNT         3229 a         6725 c         6976 g         3283 t
ORIGIN      
        1 ttgagcggcg cgttgaggga atggtcggga gcggcaaggc ggcggttcat gctcaccacg
       61 tccggctccg acacacccag ctcgttggcg atgcgcttga cgttctcggg cgagagatcg
      121 ccttcctcga tcgcctgcag ctggcccttg agcttgcgca gattgaagaa gagcttcttc
      181 tgcgcagcag tggtgcccat ctttaccagc gaccaggagt gcaggatgta ctcctggatg
      241 gccgcgcgga tccaccacat ggcgtaggtg gcgaggcgga agccgcggtc gggatcgaag
      301 cgcttcaccg cctgcatcat gccgacattg ccttcggaga tgagttccga gagtgggagc
      361 ccatagccgc gatagcccat agcgatcttg gcgacgagcc gcaaatggct ggtgacgagg
      421 cgatgcgcgg cgtccggatc ggcatgctcg cgccagcgct tggcgagcat gtattcctct
      481 tcgggtgcca gcatcgggaa tttgcggatc tcctggagat accgcgccag attcccttct
      541 gaggcaatgc tcgggagcgc cgaagtcgcc atggcgttct taagtccccc tggcgctgct
      601 tatcgtctcg tggcccaccg gccgcagcgc gatcactaca acacatctta gaccaggaac
      661 ggttttctgt ccaatttaga cagtatctaa taagtgtacc aactccttta tatcgctagg
      721 caacttacta ctaaattcaa tattttctcc ggttgaagga tggcggaagc cgagcagcac
      781 cgcgtgcagc gcctgacgcg ggaacgcggc gagctgagcg cgcggctcga gcgagagggc
      841 gcgcaagcgg ccggggctgc gcggattgcc gtagacgggg tcgccgatca gcgggtgacc
      901 cgcggcgctc agatgcacac ggatctgatg cgtgcggccc gtcgccaggc ggcattcgac
      961 gagcgcagcc acgtccttga aagcgcggag cacgcggtaa cgtgtgaccg ctggctttcc
     1021 gccttggcgc agcacggcca tcttcttgcg gtcgcgcggc gagcggccga tgttgctcgc
     1081 gatctcgccc gcacgcggct cgggcacgcc ccagaccact ccctgatagg cccgcgtgat
     1141 gcggcgcgcg gcgaaatcgg cggcgagctt cgcttgcgcc agctcgtcct tggccacgac
     1201 catgagcccg ctcgtgtcct tgtcgaggcg atgcacgatg ccggggcgct tgacgccacc
     1261 gatcccggcg aggctggcgc cgcaatgggc gaggagcgcg ttcaccagcg tccgatcggg
     1321 attcccgggc gctggatgaa ccaccagccc cgccggcttg tcgatcacca ggagatgtgg
     1381 gtcctcatag acgacggtga gcgccatcgc ctggggcacc ggcgtgtcgt cgaccgctgc
     1441 cgggaccgtc agtacgaagc tctggcccgg cttgacgcgg agcgacgggt cgcggaccgt
     1501 cgcgccgtcc gccgtgacgc ggcccgcctc gaccaggttc ttcacgcgcg tgcgcgagag
     1561 atcgtcgagc cgcgccgcca gcagccgatc gagccgctcc cccgcatcgg ctggctccgc
     1621 cgtgacacgg cgagtcgtct cgctctcgct catcgagcgc gccgccgaga accatgcggt
     1681 tcggcgcggc gcccccgacc ctcaagggga tgatccaccg catgtccgga ttccctcccc
     1741 cctcgagggg gaagggcggg gaggggggta tgttgccggt ccgccattgt ctgggagagt
     1801 gtatcgcttc ccgcgattgc tttacatttg ccgagaccat gaacttccgc agcgacaacg
     1861 aagccggcgt ctcgccgcag atcctcgcgg cgatcgccgc ggccagtcac ggcacggcgc
     1921 cgtcttacgg cgaggatgag atcacggcgc gtctggcgcg tcgcttcgcc gaaatcttcg
     1981 agcgcgacgt cgtcgtcttt cccgtggcga cgggcaccgc cgccaacgcg ctggcgctgg
     2041 cggcgctgac gccgccctgg ggcgtcgtct tctgccacga gaactcgcat gtggcgaccg
     2101 acgaatgcgg cgcgcccgaa ttctacactg ggggcgccaa gctgatcggt ctcgccggcg
     2161 gcgagggcaa gctcgcgccc gcttcagtcg agccgcatct cgtcggcatc gacgttccgc
     2221 accatgcgct tccggccgcg atcagtctgt cggaggcgac cgaggcgggc accgtctaca
     2281 gtccggagca ggtcggagcg ctgggcgccg tcgcccggcg ccaccgcctc ggcctccaca
     2341 tggacggcgc gcgcttcgcc aatgccgtgg cggcgctggg ctgcgcgccg gcggacctca
     2401 cctggcgcgc cggtgtcgac gtcttgtctt tcggcgcgac gaagaacgga gcgctcgctg
     2461 ccgaagcggt catcttcttc gatccggaga aagccgccga tttcgcgttc cgccgcaagc
     2521 gcggtggcca tcttttgtcg aaaatgcgtt tctgctcggc ccagctcgac gcctatctgg
     2581 ccgacggcct ctggctcgag aatgcgcgcc acgccaaccg catggcggcg cgactcggcg
     2641 ctggcctcgc gcgcgtgccg ctggcgcgcc tgcgccaccc ggtggaggcc aacgaagtct
     2701 tcgtggagct tcccgaagcg gtgatccaag ccctcgccgc cgccggtttc gcctttcatc
     2761 gctggcgcag cgcggcatcc tcctgcctcc gcctcgtcac cgctttcgac acgcgggcgg
     2821 cagatgtcga cgccttcatc gcggcgatgc tcgcttccgc acccccggac ggctcctcgg
     2881 agcacaagtc aacgctttaa taacccggac gcgctattca ttccggcttg agcggcattt
     2941 ttttcctccg gaggccgtag cttttcttga cggcgatggc acgtcgccgc acattttgcc
     3001 ctcgcgaaca accgggccgg agcggccgtt tcgcagagcg caggagtggt tgaccggacg
     3061 atggccaagc gaaacggcga tgggaacggt actgcggcga acggcgcgcc gaagccgctg
     3121 cccaagaagg cagccgccaa gaacggcgtt gcccggacct tgggaccggt ctccgacctc
     3181 gagcgccatc tgcccaccga gtggtggcgc acgctcttca attccctcta tctcgagacc
     3241 gacggcgacg tcgtcgagaa cgaccgcaac accgagcagg agatcgatct cctcgtcagg
     3301 agcgccgggc tcgagccgaa cgaccgcatc ctcgatctct gctgcggcca gggccgccac
     3361 gccctcgagc tggcgcggcg cggcttcaag aacgtcatgg gcatcgaccg ctcgcgctat
     3421 ctggtgcggc tggcgcgcaa gcgcgcccgc cagcgcgggc tccctgtctc cttccacgag
     3481 ggtgacgccc gcaaattccg cgtgcccgag agcagcgtcc attgcgtctg catcctcggc
     3541 aactccttcg gctacttcga ccgcgaggag gacgacatcg ccgtcctcgg cgcggtgaag
     3601 cgggcgctcg cctcgggcgg cacgctggtg atggatctca ccgacggcga gtggatgcgc
     3661 agtcacttcg aagcccgctc ctgggagtgg atcgaccaga accacttcgt ctgccgcgag
     3721 cgggcgctag ccggcgacgg cgagcggctg gtcagccgcg aggtggtcgt ccatgccgag
     3781 cgcggcgtga tcgccgacca attctacgcc gagcgcctct attcccggga gcgcctccag
     3841 acgctggtcg agagcgtcgg cttcgccaac ctccgcttcc acgccttcct ctccccggat
     3901 tcgccgcgca accaggatct ggggatgatg gcgcaccgca tgttcctgac ctgcgaggcg
     3961 ccgcgcaaag tcgagcgcca gcgccacgag gtcatcttcc ccgaagtaac cgtgctgctg
     4021 ggcgatccgc gtctccccga tccggttaag cgcaacggcc agttcaacac cgaggacctc
     4081 gacacggtcg cgcgcctcaa gagcgcgctc gccgagattc ccggctaccg cttccgcttc
     4141 ctcgacaacc acgcctcgct gctcgccgat ctgcgcgccc agcggccgga cttcgtgctc
     4201 aatctctgcg atgaaggcta caacaacgac gccttcatgg agctccacgt gccggcggtg
     4261 ctggagctgg tcgacgcgcc ctatagcggc gccgcgccct cctgcctcgg cctctgctac
     4321 aacaagtcgc tggtgcgggc gatcgccgcc tcgctcgacg tgccggtgcc gctcgagacc
     4381 tatttcagct ccgacgacca ggcggcgacg atcccctcgg tcttcccggc gctgatcaag
     4441 cccaatttcg gcgacagctc catcggcatc acccaggagg ccgtggtcta cagctgggag
     4501 caggcgatca actacctgag cgggctgcgc gagcagatgc ccggccggcc gatcctgatc
     4561 caggagttcc tcaccggtgc cgagtactcg atcggcatca tcggcaatcc cggcatggcg
     4621 ctccgcgcgc tgccgccgct cgaggtcgac tactcgcagc tcgaccgcga cctgccgcag
     4681 ctcctggcct atgagtcgaa gtggatcccg gactcgccct actggtcgca gatcaagtat
     4741 cgcgaggcgc agatcgacga ggacacgcgc cgccgcctcg tcgaccactc gaacatcctc
     4801 ttcgagcgcc tcggctgccg cgactacgcc cgcttcgact tccgcgccga tgccgagggc
     4861 gagatcaagc tgctcgaggt caatcccaat cccggctggt gctgggacgg taagcttaac
     4921 atgatggcga cctacgccgg gcttcgctac tccgacatgc tgaagctcat cctcgaggcg
     4981 gcacaggaac gcattgcggc gcagcgtccg caggtcgttg ccgccgccac gcccgagcgg
     5041 ctcgccgtcg gccaacgctg aacggccgac atctcaaatc ccatgtatcg agtggagcgc
     5101 ggctcctcgg gagcggtctt ctggcgcgct gcgtcgcgaa cagcgcgcgg cgcgcgacgg
     5161 ttgcacggca ttgtctcaaa aaaaattcgc cgctgtgaaa ttttcccttg catcgccgcg
     5221 cagtcagcgt atttatcgga atcacgacgc actcgcacgt gcctatggcc cttgtggtta
     5281 tgcaacgacg taacgcgtcc tttttggaag atccggtcga cagtgggccg gtcccgagag
     5341 ggaggtcgat atggttgcac tacgactcag ggccgcagcg ccctggcccg acgatacacc
     5401 tcggctgatc agggaagcgc gcggcagcgg tggtcgagct tccggcgaac ccagcaatct
     5461 aatctgaggc gctcgtccag cgcacggcgg atcgcccccg tgactggact acgccctttg
     5521 gcttcatcgc cctcgcggtt cgcgggggca gcggcgcagc gcaccatctg cgcgcccgcg
     5581 gctcgctagg aaagaccacg aaaatgaaga agaaaaccga caaaaaggcc gatcgtttgc
     5641 gaagaatcga ccgctttctc gccgaaaggc agccggaaac gccctgtctt gtcgtcgacc
     5701 tcgaagtggt ggccgacgcc taccaggcgc tccgcaccta cttgccgctg gccagggtgt
     5761 tctacgcggt gaaggcgaat ccggcgagcg agatcgtcga cctgctgcac ggcctgggct
     5821 cgagcttcga cgtcgcaagc cgcggcgaga tcgagctctg tctcggccgc ggcgtcgcag
     5881 ccgagcgcat ctccttcggc aacaccatca agaaagagac ggacatcgcc tacgcgcatc
     5941 agcgcggtgt gcggctcttc gccttcgaca gcgccgcgga gctcgagaag ctcgcgcgcg
     6001 cggcgccggg tgcgcgcgtc ttctgccgca tcctcgtcga cggcgagggc gccgagtggc
     6061 cgctgtcgcg caaattcggc tgcgcgcccg agatggccgt cgagctcctg cgccgcgcca
     6121 aggcgcaggg cctcgacccc tacggcgtct ccttccatgt cggctcgcag cagacgaatc
     6181 tggagcaatg ggacggcgcg gtcggccgtg cggcgcgcat gttctcgctg ctggcccaga
     6241 cggacatcaa cctgcgcatg gtgaacatcg gcggcggctt ccccgcgcat taccggggcg
     6301 acgtgccggg cgtcgagcgc tatgcggcgg cggtgatggc ggcgatcacc cggcatttcg
     6361 gcaacgacct gccggagatc atcatcgagc cgggccgctc gatggtgggc gatgccggca
     6421 tcatccagag cgaggtggtg ctgatctcgc agaaggcctt cggcgacgag aagcgctggg
     6481 tctatctcga cgtcggcaag ttcaacggcc tcgccgagac gatggacgag agcatcaagt
     6541 accgcatcaa gacgccggcc gatggcggtc ccaccgggcc gacagtgatt gcgggcccga
     6601 cctgcgacag cgcagacatc ctctatgagc ggacggagta ccgcctgccc atggccttga
     6661 aggttggcga caaggtgcag atcctctcga ccggggccta cacgtcgagc tacgcgtcgg
     6721 tcggcttcaa cggcttctcg ccgatcaaga cctactgcat ctgagactga aacgtggcgg
     6781 cgtggcgctc gcgccgcgcc gccgcgactt ccgccagcag cttctgctgc atggcctggg
     6841 caaaggacgc gaagtcctcg acctgcatca ggaagccgcc gggaccgccc accacatact
     6901 ggtcgtaata gctgctgagc caaggctcgg tcttgaggat ggcgaggccg ttgatggtga
     6961 tgcccgctgc caccatctcg tcgcgcaccg cttgcggcgg cggcccgata ttggcgatgc
     7021 cgtcgcccga gacatccacc acctgacgcg ccgccttgtc cggaagcaac cggaaggcgg
     7081 cttgcgctgc cagcagcgca ttgccgatgg cggtaaggcc gctcgagctg cgcttgctcg
     7141 cgcgaagctt ggccgtgaag tccgcggcgc tcgccttgtc ggagacgcgc atccagtcga
     7201 ccgtcacgac ctgcttttcg ggatccgacc actcaaggac gagaacgtcg atcgcgccat
     7261 gggcgccgcc cttgatcgcg tcgagcagcg gctggctctc gaaggcgcgg gcgatccctt
     7321 cgtgttgcag ctcgtactcc tccgcatcga cgctctccga gacgtcgacg gcgaagacca
     7381 gggcgagatc gaccgttgcg gcgcgcgcgg ccagcggcgc gagcagcaag ctggcgagca
     7441 tgagcgcggc ggtggggcga gcgaccggca ttggacctcc tgacgagacg gagcaggaga
     7501 cgcggccgcc tcccgtcacg atggctcgaa atataggtga tccaggacgg cggttcagcg
     7561 ccacgaaccg cctgattgcc cccgcggacg ggcgcggagg gcccgctcgc cgcggctaat
     7621 tcgtcttccc cgcgtcgagc gaacggccgg cggcggagag cggttcggag cggcccgtca
     7681 ggcgggcccg ggcgtcgcgg tcggcatgcg acggcagcgg cgccgcatga tcggcgaccc
     7741 aggcggcgac gtcgccaacg ggcagcgatg cgtcgagatc gcgcagcagc atgtgatagc
     7801 cacgaggata gagcgcaacg cgctgccgca gctccgcctg tggcggaagg gcgcggacga
     7861 agcgctgcat cggcgcgcgg ggcacgatct cgtcatgctc gccatagagc aggagggccg
     7921 gcgcgtcgaa ccaggcgccg gcatgaaggg ccgcgctcat cagatcgacg aggccgttga
     7981 tcgcgtcggc ccgcgtctcc acgatcacca gcggatcggc attgaaggcg cgcagcatgg
     8041 cgatattgtc ggagggcatg atctcgatcg gaatgatgtc gggagagaag ctggcgtccg
     8101 gcatcaggcc ggcgaaccag aggctgacgc gctcgaggag agtcatggtc tcgcgacccc
     8161 atacggccgg cgccaccagg atcacgccgt cgaccgccgg caccggcgcg ccgctcttgc
     8221 ccgacgcggc cagcaccgcg accgcaccac ccatgctctc gccgagcaga taaagaggca
     8281 cgctcggata gcgctggcgc aagaggcgcg ccgcacccgc ggcgtcttcg gcgaggcgct
     8341 gcccgccgac ccagcgtccg cgaccgggcg ccgcgccgaa gccgcgttga tcataggcat
     8401 aggtcgcgat gccgagccgc gcccacgccg cgccaggccc ggcaaaggcg ttcgagtagt
     8461 cgttgaagcc gtggagcgcc agaatgacgg cggcaggcga gcccggcggc agccatcgcc
     8521 gcagcggcag agcagcgcca tcggcggcga cgaaccggtc gccggtgagc tcgggagcgt
     8581 tcgcagcggc gctgcgcggg gcgggaggcg gcgcgtcgtg gggagcgatt gcagcagcgc
     8641 agccggccag cagcgcggcg acgatcgcga cggcaagaaa gcgcatggcg gctccgacca
     8701 aggaggctgg aagcatcgcc ccaccgtggt taaccggaag taaaggcgcg ccgcgccggc
     8761 ggtcttggat gatcggaaac gcgggacggc tcagcgcacc atgccggcgg tagcgacggg
     8821 gcgcagcgca gcggccggcg atgtgccgag cgcacgcgcg tagagcgccg ggtcgtcgag
     8881 cacgcgtttt gccgccatga cctcgacatc gtggtcctcg cggtcgccgg ggcagagcgc
     8941 gcggaggcga cgccagccgg cctcgtcgag actcgcgcgc ggacgcacca gccctgtgct
     9001 ttcatcacgc agcgctccgc cgaggcctgc cccatcctcc ggcaggttcg ccgtgcacac
     9061 cgtcggaacg acgccgtgct catgcaggac atagccgccg ggcgtgagaa ggcgggccca
     9121 ggtcacggtc atctccgcgt cgttgctgag atgctggacg ttctgcaccg tgcccttgcc
     9181 gaaggaggca gtgccgacga cgacggcgcg gccggtgtcc tgcagcgccg cggcgacgat
     9241 ctccgaagcc gaggccgagc cgccattgac cagcacgacc atgggcaatc gctccacgtc
     9301 gcgatgcggc gcggcgaagt attggatgct ttcggggacg cggccgatgg tcgagctcac
     9361 cggcgtcgcg tcgaggaaga ggctcgccac ctcgaccgac tggtcgagca gcccgccggg
     9421 attgccgcgg agatcgagga tgagcccgcg caggctggcg cccatgtcgc gatgcgcctg
     9481 ctggagcaga tcggcaacgc tcggcgcggt ttgctgattg aagccggaaa gccgcagatg
     9541 cgcgatgccg ttctcttccc ggagcgtgac ggtgggcggc acgatatgcg cgcgccgcat
     9601 ggagaaattg aggcgcttgg gcgagggcgc gcgcgtcact gcgagagcga tgaggctttc
     9661 gttgggtccg cgcagtcggc ggacgacctc ctcgcgcgtc agggccgtgg tctcgacgcc
     9721 gtcgatcgcc aggatgcggt catcgacctg caagcccgcc tccgcggcgg gtgtctccgg
     9781 catgatctgg acgacgcgga tgccgtcgcc ctcggcgttg agcgtgacgc cgatgccgcc
     9841 gaagccgtcg cgcgacgcgc gccgctcgcg cgccaactcg ggcgcggcgt agtgggaaaa
     9901 gcggtcgaga atcgtcatgc tgccttcgac gaccgtcgcg tcgaggccgt cgggtgacaa
     9961 ccccgcgacc gcgggcgagc gctcgcgcgc ccagttcagg accgtctcgg tgagctcggc
    10021 ccattgctcg gcgtcgcgcg catcgggggc cgaaaaatgc gtgctcgaca ggccatgatg
    10081 ggcgacgacc tcgctgcccg accattcgac cgacagcgtc gcatcgatgc tcgtgagatt
    10141 gcgcaggccg gcgatcgcca gcgcccccgg ctgcaccggc tcgatatagt atttggcgat
    10201 accgctatag gcttcctggt agaagcgcgt ggtgtcgttg gcgatggcgc ccgagggcgg
    10261 cgcaccgccg caactggcca gcgccaggat cgccagcagg gccgcggcac cggcggcaga
    10321 tccggcgatg ccgtgtcgga ggggcgtcgt gggggctttc gacggtgacg tctgcaagcg
    10381 acctccctgg cccagccgac aagccgagct cgcctaacct taggcgatta tcgcaaaatt
    10441 acgttaacgt ctagttgagg aacggcgacc cggaggccgg tccgtttcct cgagcgcgaa
    10501 gagcaggctg ccggtgaccg tgttggcctc ggcgagccgc accgtcacgg catcgcccag
    10561 cgtatagacg cgaccgaagc ggcggccgac gagacgatgg cgcgcttcgt cgtgattata
    10621 gtagtcgtcc ggcaggctgc gcatcggcag gaggccatcg gcgccgctct cggccagcgt
    10681 cacgaacagc cccgcgcgcg tcacgccgtt gatccgaccg gggaagctcg cgccaacccg
    10741 ctcggcgagg aaagcggccg tgtagcggtc gctcgcgctg cgctcggcgg cggcggcgcg
    10801 gcgctcggtc atcgagatgt gatcgccgct cgcggcgaac tccttgggct cggtcggcgg
    10861 caggccgccg gcgccagcgt cgagtccggc gatcagcgcg cggtggacca ggagatcggc
    10921 ataacggcga atcggcgacg taaaatgggc gtatcgcgtc aggcccaggc cgaaatggcc
    10981 gatgttctcg gggctgtaga tggcctgggc ctggctgcgg agcaccagct cgttcaccag
    11041 gctctgatag ggcgtgccgg cggcgcggcg caggatctcg ttgaaatggt gcggacgaac
    11101 cagctggccc ttggcgagct tgaggccggg gatgccgatg ccttcgagga attcgcgcaa
    11161 cgccgagagc ttcgccgggt cgggagcgtc atgcacgcgg tacatgcagg gccggcgcag
    11221 ccgctccagt tcctgcgcgg cggcgacgtt ggccgcgatc atgaattcct cgatcagctt
    11281 gtggctgtcg aggcgctggc gcggctcgat gcgcgccacg cgtcccgacg gatcgagcag
    11341 gatgcgccgc tcgggcaggt cgaggtcgag cgtcccgcgg gtgcggcgcg cgcgctccaa
    11401 cgcgtgatag gcgccgtaaa gcggccgcaa cacctcgagc acggatccgg cggtgtcgtt
    11461 gggccggcca tcgaccgcgt cctgcacggc ctcataggtg agccgcgcgg ccgagcgcat
    11521 gaggccgcgg aggaagcggt ggcggtggac cttgccttcg gcgtcgagcc agagatggac
    11581 ggccatgcag gcccgctcga cgcccggctt caacgagcag agttcgttgg acagcgcctc
    11641 cggcagcatc ggcacaacgc gatcgggaaa atagaccgaa ttgccgcgct cccgcgccgc
    11701 gtggtcgagc gcgtcgccgg gacgcacata ccaggcaacg tcggcgatgg cgaccaggat
    11761 atgccagccg ctttggcgct cgggatcggg ctcggcccag accgcgtcat cgaagtcgcg
    11821 cgcgtcggcg ccgtcgatgg tgacgagcgg gatcgcgcgc agatcggtgc ggttgccgag
    11881 atccacgggc cgcgccttct cggcgagcgt tagggcctcg ggccggaagt cggtgggaat
    11941 accgtgggca tgaatggcga tgaggctgaa ggcgcgcgga tcggcgctgt cgccgacgcg
    12001 ctggacgacg cgcgcttggg gcagaccgag gcgccgcgcc ggcagcacct cagcaagtac
    12061 gatctcgccg tcctgggcat cggccgtgtc gacatgggcg acgcggaact cggtccgcag
    12121 cttgcggtcg gtgggctcga tgcggccgcc gtcgcggtcg cgtcggaaaa cgccgaggac
    12181 gcgatcgggc gccgcgccga tcgcgcgaat gacgtgtgcc tcgtaggtgc cgtcgtcggc
    12241 ccgcacgaag cgcgcgacgg cgcgttcgcc gacgcccagg gcaggggcgc cgcggtcggt
    12301 gacgataaag aggatcggcg cgttttgcgc gtcgcccgag gcgacgggct tgccgtgcat
    12361 ctcgccatcg aggtcgatat cggtgatctc gatgacgccg atctccggcg ggctgccgac
    12421 cggctggagc cgccgcttgc ggccgcgctc ggccgaaccg gcgcgctcga tctcgcggac
    12481 gagctccttg agggcgatgc ggtcggcggg cggcacgcgg aacgcgcgca cgatgtcgcg
    12541 cttgccgacc gggatcgcac tcgctgcaat gaattcgagg agctcttcgc gggtcggcag
    12601 tcgtcccgcg gcacggggcc gcttgttctt cgccgccgcc tcgccctggt cgttactcgg
    12661 cggctttgcg cgtcggccgt gcttgcgtct ttttgcgggc gcccgccttg gcacgggtcg
    12721 atgttccttt cttctcgggc ttggcggccg aggccgccgc gaccttgttg ggcttggcgg
    12781 ccggggccgc cacgacctta gctttggcct tggccttctt gccgcctttg accttgcggc
    12841 gggcggccag cagctcgagc gcctgctcga gcgtcaccgc ctccggctca gtctcctcgg
    12901 gtagggtggc attgatgccg tcgtggctga catagcgccc gtacttgccg tcgaacaggt
    12961 tgaccggcgc gccatcctcc ggatggttgc cgataacgcg aagcggcgtc ggcccgcgac
    13021 gcccgccctt cttgggctcg gcgagcaggg agacggcgcg attgaggccg atggtcagca
    13081 cgtcgtcgtc gccgcgcacc gacttataga gcttctcatg cttgatgtag gggccgtaca
    13141 tgccgatggc ggcgatgatg ggcttgcccg tctccggatg caggccgacc tcgcgcggca
    13201 gcgacagcag cttcagcgca tgggcgaggt cgatttccgt cggtgatacg cccttgggca
    13261 agcccatgcg cttgggcttc tcgtcttcgc tcgccgcgtc gcgctggacg tagtagccgt
    13321 aagggccctt gcgcacagtg acggcgaggt tgatttcggg gtcgacgccg agctcgcgcg
    13381 ggccggtcag ctcctgcccg ttgccgttgg cgttctcgat ggcgagcgcc cgggtatagc
    13441 ggcattccgg gtagtgcgcg cagccgatga aggcgccgta cttgcccagc ttcagactca
    13501 agcgcccgtt gttgcagccg gggcagagcc gggggtcgcg gccatccgcg gccggcggga
    13561 agaaatgcgg gccgaggtcc tcgtcgagag tgtccagcac ctgggtgatg gagagatcct
    13621 tggtgccggc gacagcgagg gagaaatcgc gccagaaatc ctcgagcacc ttctgccagg
    13681 caatgcgtcc gtcggagatg tcgtcgagct ggttctcgag atcggccgtg aagttgtatt
    13741 cgacatagcg ccggaagtag ctctcgagga acgcggtcac gacgcggccg cggtcctcgg
    13801 ggatgaagcg ctttttgtcg agccgcacat agtcgcggtc ctgcagcacc tggatgatcg
    13861 acgcataggt cgagggccgg ccgatgccga gctcctcgag cttcttgacg aggcttgcct
    13921 cggtgtagcg gggcggcggc tgggtgaaat gctgttgcgg atcgaccgtg ccgcggccga
    13981 gcttgtcgcg ctcgttgagc ggcggcaggc ggcggttctc gtcgtcctgc tcggtgtcgt
    14041 cctcgtcctc gcgatagagg gtgagaaagc cgtcgaagac gatggtcgag ccggtggcgc
    14101 ggaattgcag ctggccgtcg gcggacgcaa tgtcgaccgc gacctgatcg atgagcgccg
    14161 aggacatctg gctcgccatg gcgcgcttcc agatcagctc gtagagctga agctgctccg
    14221 gctcgagatc gcgcgcgacc ttctcggggc ggccgaacat gtcggtcgga cggatcgcct
    14281 catgggcttc ctgcgcgttc ttcgcctggg tcttgtagac gcgcggctcg tcggggcggt
    14341 aggccgcgcc gtattgcttc tcgatgagat cgcgcgccgc ggtgatggcc tcgctcgaca
    14401 tggtgacgcc gtcggtgcgc atataggtga tgaggccgac cgtgtcgccg gcgatgtcga
    14461 cgccctcgta gaggcgctgg gcgatgcgca tggtgcggct cgcgccgaaa cccagcttgc
    14521 gcgacgcctc ctgctgaatg gtcgaggtgg tgaagggcgc cggcgggttg cggcggatct
    14581 gcttcttctc gaccgtggcc accgcgtagc tctgctggcg caggatgcgc tcggcggcgg
    14641 cgcgtgcggt cgcctcgtcg gcgatgtcga atttgtcgag ccgcttgccg tcgatatggg
    14701 tgaggcgcgc cgtgaagcgc tcgccggacg gcgtctggaa ctcgacctcg atgccccagt
    14761 attcacgcgg cttgaaggcc tcgatctccg attcacgctc gcagatgagg cggagggcga
    14821 ccgactgcac gcggccggcc gagcggcttc cgggaagctt gcgccacagc acgggcgaga
    14881 gcgtaaagcc caccagatag tcgagggcgc ggcgggcgag gtaggcgtcc accagctcgc
    14941 ggttgagctc gcgcggatgg cggaaggctt cgagcaccgc cgacttggtc acttcgttga
    15001 agacgacgcg cttgacgtcg acgcctttca gcgccttgcg gttcttcagg acctcgcgca
    15061 catgccagga gatggcctcg ccttcgcgat cggggtcggt ggcgaggtag aggtgctcgg
    15121 cgccttcgac cgccttcgcg atggcgctca tgcgcttctc ggcgcggtcc tggacttccc
    15181 aggacatggc gaaaccctcg tccggccgca ccgacccgtc cttggccggg agatcgcgga
    15241 catggccgta gctcgccagg accttgtagc ccttgccgag gtacttattg atggttttcg
    15301 cctttgcggg cgattcgacg atgacgacgt tcattgccta ggactggtga ataaggccgg
    15361 ggacagatag ttccctaagg tcgttaaagc aaggctacca ggttccccgg ttggcgttcg
    15421 agccgtccgg cgagctcgag ctcgagcagg acgctcatcg taagtgattg aggtaattga
    15481 cattcccgca ccaggtcgtc gatccccacc ggggtgggtg acagcctttc gaggacccgg
    15541 cggcgggccg tctcgagctc gctaccccct gccggccctt ccggaggggc gtccgaccag
    15601 gcgctccggc gggccggcgc cctcggcgaa agcggcccct ggagctgggc ggtgacgtct
    15661 tcggcggtct cggtgagggt cgcgccgcga cggatcaggt cattggtgcc tcggcatcgc
    15721 gggtcgaggg gcgagccggg cacggcgaag acctcgcggc cctgctccaa ggcgaagcgc
    15781 gccgtgatca gcgagcccga ccggagcgcg ccttcgacca cgacgacgcc gagcgagcag
    15841 cccgagatga tgcgattgcg gcgcgggaag tggcggccct tcggcacggt gccgaccggc
    15901 atctcggcca gaagcgcgcc ccgctcgatg atggcgtcgc gcagcgcgcg gttttcctcg
    15961 ggatagagga tgtcgatgcc gccggcgagc acggcgacag tgccggtcgg cagcgcgcct
    16021 tcatgggcgg cggtatcgat gccgcgggcg aagcccgaga ccacgatcag gcctttctgg
    16081 ccgagctgca gcgccagatc ccgcgcgaag cggcggccgt tggtcgaggc attgcgcgcg
    16141 ccgacgaccg cgatggcggg acgcttcagc agcgcggctt ggcccaggac cgacaagagc
    16201 ggcggcgcat cctcgaccgc ggccaacgca gagggatagt cgggctcgcc ccaggcgacg
    16261 agacgggcgc ccaggcgatc gaggctttgg agctcgcgct cggcggcgcc tctggtgaag
    16321 accgtgatcg gccgatcgcg cccgccgcgg cgcgcaatgt cggggagcgc gtccaaggcc
    16381 gccgcggccg agccgaagcg cgccaatagc tggtagaacg tgacgggacc gacattctcg
    16441 ctgcggatca agcgcagcca gtcgagtttc tccgccggat tgaggacgcg cggggccgcg
    16501 gacatggccg ccaccatgtc gcgaaccaag cggaagcgtc aacggcccag agcttcgttg
    16561 atatatttcg ggttgccaag caatcggggg agcccgtcca tgccagcatc gccggtcggc
    16621 gtcgggcggt cggcgcgatg tcgtcaaatt tctctcccct tgcgggggag cggtagtgcc
    16681 tcagttcgca caagcgcctt aaggggctgg acccggcgac ggctgcgggt gtgtcccaat
    16741 cgctttggtc agtgggtgat atcgtggact tgctcgagcg cactgaagcg atggaagatg
    16801 cctctttgcg ggtcggatag ctatggaact cggctggggg cctaagttcg ttctacatgc
    16861 catgcggttc atcgctgctt ggatcatcgc cggctgggct ctcctcctac tttgcgtcct
    16921 tgtggattgg tttgccaaac ggggatgggg ttatccgtgg tggtcagcgc cgttcttgct
    16981 cggcgggatc gcgattgccg tcttgattcg gcgcgcagcg gcatgggcgc tcgcccgctt
    17041 ttagccgctg ttacaatcaa ctgaggcact ctcggaaaag cgagaaactg gaccaatccg
    17101 tgcgatcctg cttagtgtct agtgcgatga cgcgcaccct catcaaatcc ggaatgatcg
    17161 tcacgctcga ccgccggatc ggcgatctgg cgcaaggcga cgtgctcatc gacggtgagc
    17221 gcatcgccgc cgtggcaccg gccatcgccg ccgacgatgc gcaagtgatc gacgcccgcg
    17281 acagcatcgt catgcccggt ctcgtcaatg cgcatatcca tacctggcag acgggcttgc
    17341 gcggcgtcgc cggggactgg acgatcctcg agtatcttca tcacatgcac gcgtcgatcg
    17401 cgccgcattt caaacccgac gacgtctacg tcgccaatct cgtgggtgcg ctcaatcagc
    17461 tcaattccgg cgtcaccacc atggtcgatt ggtgccacaa caatccgacc ccggcgcata
    17521 gcgatgccgc gatcgacggc ttgaaagaag cgggcctcag ggcggtcttc ctgcatggct
    17581 cgcccaagcc cgacgtgaag ccggggcaga agcatttcag cgagattcct catccgcgcg
    17641 tcgagatcga gcgcctcgcc cgtgatcgcc tgccgtcgcg cgatggcctg gtgacgctcg
    17701 gcatggccgt cctcggcccc gcctactcca cctatgaggt gagccggcat gacctggcgc
    17761 tcgcgcgcgc gctcggcatg ctgtgcagca tgcatgtggg cggcggcgcg atgcggacgc
    17821 ccgacggctt tgtccgtctc gcgggcgagg ggctcatcga ccggcgcgtc aacatcgtgc
    17881 atggcaacaa tctcgccggc gatcatctcc gcgcgctgct cgacaacggc gcgagcgtca
    17941 cggtgactcc cgaagtcgag ctgcagatgg ggttcggcga ttgcctgacg gggcggctgc
    18001 gcgaggccgg gcgcgcgccc tcgctcggct ccgatgtcga atcgagcatg ggcggcgaca
    18061 tgttcaccac catgcgcatg gcgctgcagg cgcagcgcgc catcgacaac gcgaaagtca
    18121 tcgcgcggag cggcggcgcg cccgagcatc tgacgatcgg ctgccgcgag gcgctcgaat
    18181 gggcgaccat cgagggcgcg cgcatggccg ggctcgacga ccggatcggc tcgctcacgc
    18241 cgggcaagca ggccgatatc gtcctgctgc gggccgacga cctcaatctc tgtccggtgg
    18301 tcgatccggt gcggagcatc gtcctccacg ccggcgccgc caatgtcgat accgtcatcg
    18361 tcgcgggaag cgtcttgaag cgcgacggcg cgctgctgcg gaacgacctc gcgcgccgca
    18421 aggccgagct cgtgagctcg agccggcgca tcctggcggc gccagtcacg ctctaaaaaa
    18481 gacgggaagg gagcacatca tgcgcggcaa catctgggga ttggccggtc tcctcctcgt
    18541 ttcgatcgcg atcgcggcac cgcccgcacg agcggccgag ccgctcaaga tcggcttcag
    18601 catgtccttg accggcaacc tcgcgagcgg cggcaacagc gcgctcgtgg ccatgcagat
    18661 ctgggccgcg gacgccaacg ccaagggcgg gctgctgggc cggccggtgc agctcgtcta
    18721 ctacgacgac cagagcagct cggccaacgt gcccggcatc tatgcgaagc tcatcgaggt
    18781 cgacaaggtc gacctcgtgg tctcgggcta cgccaccaac cagatcgcgc cggcgatacc
    18841 catcgtcaag cagaagaaca tggtgttcat ggcgctcttc ggcacgggcg tgaacgaccg
    18901 gttcaactac gacaagtatt tccagatcct gccgaacggc aaggaaacgc gcttcggccc
    18961 gtcgctcgga ttcctcgaga ccgcagtcac gatgactccg aagcccaaga ccatcgccct
    19021 cgtcggcgcc gatgccgaat atgcgcagac cgtgctctcg ggcgcccgcg acaaggcgaa
    19081 ggagctcggc ctcaccatcg tctatgacaa gagctatccg ccggccacgg tcgatttcgt
    19141 gccgatcctg cgggcgatcc aggcgaccgc gcccgacgtc gtctatgtcg cctcctatcc
    19201 gcaggatagc gtcggcatgg tgcgggcgag ccacgaggtg gggctcaaga cgaagatgtt
    19261 cggcggcggc atgatcgggc tcgccttcgg gccgatcaag cagcagctcg ggccgctgct
    19321 caacggcatc gtgtcctacg acgtctatgt ccccgagccc accatgaagt tccccggcat
    19381 cgaggccttc ctcgagcatt atcgcgaaaa ggcgatcgcc gcgggcatcg atccgctggg
    19441 cttctacctg ccgcctttcg cttacgccga gatgcagatc ctggggcagg cggtcagcga
    19501 cgtcggcagc ctcgaccagg cgaagatcgc cgagcacatc catgcaaccg ccttcgacac
    19561 catcgtcggc cgcgtgaaat tcgccgagaa cggcgagtgg gagacatcgc gcatcctctt
    19621 cgtccaatat caggggatcc agggcaacga cgtcgagcag ttcaagcagg cgggcaagca
    19681 ggtgattctc tatccgccgg aactccaatc cggcaagttc ctctatccct acacggagtt
    19741 ccagcagccg tgagcctcag ctcttgccaa ggcggatcgt gctttcctcg cccttcagca
    19801 gccggcgaat gttggcggcg tggcgcagca ggatcacgat ggcgaggaag aggacgactt
    19861 cggtcggctg gatctcgcgc gtgatgagcc aggcatagag cggtgccagc accgccgctg
    19921 ccagcgccgc cagcgaggag tagcgggtga ggacggcgac caggagccag gtgccgagcg
    19981 cggcgaggcc gacgggccag gcataagcga gaaggatgcc gcccgccgtt gcgacaccct
    20041 tgccgccttt gaatccgagc cagaccggga agacgtggcc gaggaaggcg gcgccggcgg
    20101 cgagcgctgc catgtcgggg ctgaaggcgc cggcgatcag cacagcggcg gcgcccttgc
    20161 cggcatcgag cagcagcgtc aacgccgcca gcacccggct gcccgtgcgc agc
//
LOCUS       DASYNS010000271         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3184409, whole genome shotgun sequence.
ACCESSION   DASYNS010000271 DASYNS010000000
VERSION     DASYNS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3184409"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VGP50_08500"
     CDS             <1..489
                     /locus_tag="VGP50_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027457.1"
                     /translation="FEQALRDMAQLRAQRRQAREAASQQPQTPGSPFDRLSQRADNMS
                     KFSAALKRVADTGAPLYTSLNDAQKDRFKMLSHMLRGHHHMHASGDGRGFGRDGGGWR
                     GGHRFGGDGGGWRGEQRFGGDGRWREGQGFGNEGHRFGHDRGRIHELMGNDNGDEQES
                     QL"
     gene            complement(618..1655)
                     /locus_tag="VGP50_08505"
     CDS             complement(618..1655)
                     /locus_tag="VGP50_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV8027458.1"
                     /translation="MIFRQLFDSTSGTYTYLLASRHGGEALIIDPVLERVDRYLQLVR
                     ELDLRLVKAVDTHLHADHVTGLGALRDRTHCITVMGEATHADVVSMRVAEGDRVEIEG
                     VRLDVLYTPGHTDDSYSFLLADRVFTGDTLLIRGTGRTDFQNGNARAQYDSIFNKLMK
                     LPDETLVYPAHDYKGDTVSTIGEEKQFNPRLRVKSIDEYVDLMNNLKLPNPKMMDVAV
                     PANMRVGLRQEEVARKGWALSAAEAMALRGRTDVAIVDLREKSEREKHGVIPGSLHAP
                     YPSLPEHIAAGGMLHELAAATGKRIVFYCAFGERSAMAVEAAQNAGLLTACHIEGGID
                     AWKKAEGPLAR"
     gene            complement(1773..>2113)
                     /locus_tag="VGP50_08510"
     CDS             complement(1773..>2113)
                     /locus_tag="VGP50_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8027459.1"
                     /translation="VTIASTRASMSEPNTESYSATKGGILALTHALAISLGPEVRANC
                     VSPGWIETKDYGALRRKDHTQHPAGRVGRPQDIAEIVAWLLDGERSGFVTGANFVIDG
                     GMTRKMIYEE"
BASE COUNT          339 a          688 c          723 g          363 t
ORIGIN      
        1 ttcgagcaag cgctgcgcga catggcgcag ctgcgtgccc agcgccggca agcgcgtgaa
       61 gccgcgagcc agcagccgca gactccgggc tcgccgttcg atcgcctgtc gcagcgcgcc
      121 gacaacatgt ccaagttcag cgccgcgctc aagcgcgtcg ccgacaccgg cgcgccgctc
      181 tacacgagtc tcaacgacgc gcagaaagat cgcttcaaga tgctgtcgca catgttgcgc
      241 gggcaccatc acatgcacgc gtcaggcgat ggccgcggtt ttgggcgcga cggcggcggc
      301 tggcggggcg ggcaccgctt cggcggtgac ggcggcggtt ggcgcggcga acagcgattc
      361 ggcggcgacg gccgttggcg cgaaggtcag ggcttcggaa acgagggtca ccggttcggg
      421 cacgatcgcg gccgcatcca cgagctgatg ggcaatgaca acggcgacga acaggagtcg
      481 cagctctgag ccgcccagtc gccggcaagt cccggccatt gcagagcgtc tgctgatcct
      541 cccttcagcg ggcgtaaaag cggaagccgc aggctcgccc ctgcggcttt cacgtttccg
      601 agcggacggc gccgcagcta gcgcgcgagc ggtccttcgg ccttcttcca ggcgtcgatg
      661 ccgccctcga tgtggcaggc agtgaggagg ccggcgttct gcgccgcctc gaccgccatc
      721 gccgagcgct cgccgaaggc gcagtagaac acgatgcgct tgccggtcgc cgccgccaat
      781 tcgtgcagca tgccgccggc ggcaatgtgc tcgggcaagc tcggataggg ggcgtgcagc
      841 gagccgggaa tgacgccgtg cttctcgcgt tcgcttttct cgcgcagatc gacgatcgcc
      901 acgtcggtac ggccgcgcag cgccatcgcc tccgccgccg acaacgccca gcccttgcgc
      961 gcgacctctt cctggcgaag gccgacgcgc atattggccg gcacggccac atccatcatc
     1021 ttcggattgg gcagcttcag attgttcatc aggtcgacat attcgtcgat cgacttgacc
     1081 ctgagccgcg ggttgaactg tttctcctcg ccgatggtcg agacggtgtc gcctttgtag
     1141 tcgtgcgccg ggtagaccag cgtctcgtcg ggcagcttca tcagcttgtt gaagatcgaa
     1201 tcatattgcg ctcgcgcgtt gccgttctgg aaatcggtgc ggccggtgcc gcggatcagc
     1261 aatgtgtcgc cggtgaacac gcggtcggcg agaaggaacg agtaggaatc gtcggtgtgg
     1321 cccggtgtgt agaggacgtc gaggcgcacg ccctcgatct cgacgcgatc gccctcggcg
     1381 acgcgcatcg agacgacgtc ggcgtgcgtc gcctcgccca tgacggtgat gcagtgggtt
     1441 cggtcgcgca gcgcgccgag cccggtgacg tggtcggcat gcagatgcgt gtccacggcc
     1501 ttgaccagcc gtagatcgag ctcgcgcacg agctgcaggt agcggtcgac ccgctccagc
     1561 accggatcga tgatcagcgc ctcgcccccg tgccggctgg cgagaaggta ggtataggtg
     1621 ccggacgtgc tgtcgaagag ttggcgaaag atcatgcgcg ccctccttat acgcatcgta
     1681 gcccgggttg agcgctagcg aaacccgggg gctttcaagc cggtttgacg ctgccctgga
     1741 tttcgcccgg cgcgatccgg gctactccgc tgctactcct cgtagatcat cttgcgggtc
     1801 atgccgccgt cgatcacgaa attggcgccg gtgacgaagc cggaacgctc gccgtcaagg
     1861 agccaggcga caatctcggc gatgtcctgc ggcctgccga cgcggccggc gggatgctgg
     1921 gtgtgatcct tgcggcgcaa ggcgccataa tccttggttt cgatccagcc cggactgacg
     1981 cagttcgccc gcacctccgg gccgaggctg atcgccagcg cgtgggtgag cgcgaggatg
     2041 ccgcctttcg tcgccgaata ggactcggta ttcggctccg acatcgacgc gcgcgtcgag
     2101 gcgatggtga cga
//
LOCUS       DASYNS010000272         9568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5367550, whole genome shotgun sequence.
ACCESSION   DASYNS010000272 DASYNS010000000
VERSION     DASYNS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9568
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5367550"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VGP50_08515"
     CDS             <1..187
                     /locus_tag="VGP50_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8027460.1"
                     /translation="KELIAAMETAEIDSPRGKFTLSKAHNPVQDMYLRVAQGMENRYV
                     SIAVKALADPAKGCRMA"
     gene            314..1189
                     /locus_tag="VGP50_08520"
     CDS             314..1189
                     /locus_tag="VGP50_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8027461.1"
                     /translation="MPDTLIFFLIQVLNGVQEGLLLFLVTGGLTLIFGIMRIINLAHG
                     SFYMLGAYLAFALAQSLGSFFLAVLIALPIAALLGLLLEAVLFRFLYGRDHLYQVLMT
                     YGLILVLEETRSLLFGDDVHGVAVPAILDRALPLTDTLGYPYYRLALSVCCLGVAAAL
                     YLLIRRTRLGMIIRAGAVDREMVQSLGIDIGRVYRFVFALGVALAAFAGMIAAPVASV
                     YPGMGNQVLILSFVVVVIGGIGSIKGAFLGSLLIGLADTFGQVYAPEIAGMTVYMLMA
                     AVLLWRPAGLFGRAV"
     gene            1189..2178
                     /locus_tag="VGP50_08525"
     CDS             1189..2178
                     /locus_tag="VGP50_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8027462.1"
                     /translation="MVGSAEPGRIPGRHWPALLLLLCVLALAAFPAVANRFYLQHIPT
                     IMIMAIFAMSLDLLVGFTGQVSFGHAAFFGIGAYALFLASPAELAPSLWLSLALALAA
                     AAAAAFVIGLLVLRSAGVYFIMATLAFSQMLFYYAARAKWLGGSDGAYIYLKPDTGLA
                     FFDLADKRHFYYLVLALMVAVYAFLRVLLRSPFGHALRGIKDNEPRMRSLGFATFRLK
                     LLSFVIAGTLAGLAGYLDAAQFGVVNPDLLGWRLSGTVLMMVILGGMGTLYGPVLGAF
                     ALVLLQDFLSDQTKHWLLPMGLFIILAVLVLPDGLGGLIAWASRARRSAVPRG"
     gene            2171..2929
                     /locus_tag="VGP50_08530"
     CDS             2171..2929
                     /locus_tag="VGP50_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027463.1"
                     /translation="MAEPVLGVEGLSKHFGGLAAVSDVSLELHQGAIHAVIGPNGAGK
                     TTLLNLLSGELTPSSGRVRHEGRDIAGLSPERIARLGIARSYQKSNIFGGFTVFENCR
                     LAAQAALAPGLRCFRPAHSYGAVNAAASRALAEVGLAGRGAAVAATLSHGEQRQLELA
                     MVLATAPKVLLLDEPLAGMGAEESARMTALIARLEASHAILLVEHDMDAVFSLARRLT
                     VMVNGRVLESGTPEAIRRSPAVREAYLGAAEDVS"
     gene            2926..3642
                     /locus_tag="VGP50_08535"
     CDS             2926..3642
                     /locus_tag="VGP50_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027464.1"
                     /translation="MSAAPLLEARGLHTFYGSSHVIDGVDFTIGRGETVALMGRNGMG
                     KTTLLRSMLGLTPPRAGEVRVKGALMTGVKPHRIARQGIAFVPEGRGIFATLSVREHL
                     VMAARPGSDGRRDWTLPRVLETFPRLAERLDHGGTQLSGGEQQMLTIARALMTNPDLL
                     MLDEATEGLAPLIAREIWSILGRVRASGIAAVVVDKNVAKLAAIADRIVVLVKGRVAF
                     AGTAAALKAQPELMHRHLGV"
     gene            complement(3648..5360)
                     /locus_tag="VGP50_08540"
     CDS             complement(3648..5360)
                     /locus_tag="VGP50_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEV8027465.1"
                     /translation="MPRSLSPNSNIDALRRDAKRWLKSVAAGDPEAAARFRRVFPDHA
                     GVPKLREMQQALARQYGFPSWAALKQEIEDRGRGAADRVRLFLEKSVNRYGVDPSTRK
                     WGDYERDGFARGAVAARLLARHPEIAPESIHTAVAAHDLDALRALLSKDPGLVSDRCA
                     FDGWTPLLRLAYARLPMNTVATNALAIATLLLDAGADPNAFWSDGANAFTVLVGVIGG
                     GEAGQTAHPAAEAFVRLLIARGADPFAPQALYNTSLGPDSTFWLDLLWAESEKRGETR
                     KWTGPAPGELGGDRSTSALAYLLGNAVPDHIRRIRWLLEHGADASGINFYSREPVIKH
                     ALLAGREEVTELLLRHGATRPQLSDRESFLAAAAGGDLAAMRGLARTHPELLEQHEAM
                     FAAIRRHRADIAEALLDLSMSPDIGDDKNFRALHYTTHCGAPEIARLLLAHRAEIDPI
                     ELRHGGTPLTHALHHERADMIELLAPHSRHFRGLCFAGAVERLRELLGEEPERANRQD
                     RPGEPALFCLPDDEEKAVAVAELLLSFGADPSFRNPLGQTPAQAARRRGLDEAAAVLE
                     DAET"
     gene            5615..6346
                     /locus_tag="VGP50_08545"
     CDS             5615..6346
                     /locus_tag="VGP50_08545"
                     /inference="COORDINATES: protein motif:HMM:NF013882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEV8027466.1"
                     /translation="MQISLINLDRTPDRLAEFHRRNEHLEEVSRFRAVDGRTVDVGAL
                     TRIRLLHPSVVSTYTKGALGCALSHLALWDRAVTRDEAITICEDDAIFNRGFVEVAER
                     LIGTLAADWDVILWGWNFDSVVCFEMLPGVSPCCLSFDQDALRAGVQSFQDQRVAPQL
                     FKLHRALGLVCYTISPKGARVLRDRCLPLRQMDVHFPGLNRALYNTGIDIPMNDAYPE
                     IEAYVGFPPLVVTKNEHETTTVQRD"
     gene            complement(6362..7747)
                     /locus_tag="VGP50_08550"
     CDS             complement(6362..7747)
                     /locus_tag="VGP50_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV8027467.1"
                     /translation="MSSTTFDVSSPAKQHLPAPRLADLAHIPGSDGAPLVGDTFRFLA
                     DPYGWTKAMADRYGLVYRNRVFGVRSIALLGPEAVGLVLFDQSRLFSSLEGWGPFLGL
                     LFPRGLMLRDFDDHRLHRRALSVAFKVGPLKSYLAALNAGIAHRLAAWRERPCDFLFY
                     PAIKQLTLDLAAASFLGAALGSEVEAIKGAFIDMVAASIAVIRRPLPFTAMRRGVTGR
                     ARIVEYFGRQVPLRRERGGEDLFSQLCRATHEDGSLMSVADIADHMSFLMMAAHDTLT
                     SSLSAFVYFLAAHPEWQERLRAEVQGLGLAPAEPLPFERLEELRLTEMAFKEAMRLIP
                     PVISMPRRAMRDFEYAGYRIPAGTPVNASVLFTHRMAEHWPDPQRFDPLRFSEENARG
                     RHKFAFAPFGAGAHMCLGLHFAYMQAKCFARHLLERFDVSLAPGYAPKWQMAPIPKPK
                     DGLKVTFTRRD"
     gene            7931..8707
                     /locus_tag="VGP50_08555"
     CDS             7931..8707
                     /locus_tag="VGP50_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8027468.1"
                     /translation="MQREFVTAGGHRLEYQRIDGRADAPCLVFLHEGLGSVALWRDFP
                     AKLAQAAGAPAVVYSRHGYGASDRLTAPRDVDYMHREALDTLPALRAALGLGEVILIG
                     HSDGASIALIHAGSGRWPVRALILEAPHVFVEDVSVESIAAARTAYAATDLRRRLERY
                     HGDVDGAFRGWNDIWLAPAFRRWNIEEYLPGVRTPVLAIQGADDEYGTLAQIEAIERG
                     VAGPFERLVLPHCRHSPHRDQEDAVLAAMAGFIARLADHR"
     gene            8736..9485
                     /locus_tag="VGP50_08560"
     CDS             8736..9485
                     /locus_tag="VGP50_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8027469.1"
                     /translation="MRNRLVVVTGGSRGIGAAVARQAAASGYAVLVNYRDDSDGALRV
                     VREIRGAGATAEAVRGDVGVERDVLALFAAADRTNLPLAGLVNNAGMTGGFARLQDLA
                     AETLARVLTVNVVGAFLCAREAAKRMSTERGGQGGAIVNMSSRAAQLGGAGEWIHYAA
                     SKGAVDTLTIGLAKELGREGIRVNAVAPGLIDTELHGAAGDPDRVRRLASGVPMGRPG
                     LAEEVAEAVLWLISPAAAYVTGAIVPVAGGR"
BASE COUNT         1460 a         3199 c         3278 g         1631 t
ORIGIN      
        1 caaggagctg atcgccgcca tggagacggc cgagatcgac agcccgcgcg gcaaattcac
       61 gctctccaaa gcgcacaacc cggtgcagga catgtatctg cgcgtggccc agggcatgga
      121 gaaccgatat gtgagcatcg ccgtcaaggc actggccgac ccggcgaagg ggtgcaggat
      181 ggcttgagaa gccgtcagcg gtcagctatc agccgtcagc tttttgcggg atgcggcgaa
      241 cgcggggaca agctgtgtgt ctgttgcacg ttcgtttcag ctgaaagctg acggctgaga
      301 gctgacagct tccatgcccg acaccctcat cttctttctc atccaggtct tgaacggcgt
      361 gcaggaaggg ctgttgctgt tcctggtgac gggcgggctc acgctcatct tcggcatcat
      421 gcgcattatc aatctcgctc atggcagttt ctatatgctg ggcgcctatc tcgctttcgc
      481 gctggcgcag agcctcggta gtttctttct cgcggtgctg atcgcgctgc cgatcgcggc
      541 actgctgggc ctcctgctcg aagccgtgct gttccggttc ctctacggcc gcgatcacct
      601 ctaccaggtg ctgatgacct acgggctcat cctcgtcctc gaggagacgc gcagcctgct
      661 gttcggcgac gacgtccacg gcgtggcggt gccggccatc ctcgatcgcg cgctgccgct
      721 caccgatacg ctgggctatc cctattatcg tctggccctc tcggtctgtt gtctcggcgt
      781 cgcggccgcg ctctatctgc tgatccggcg cacgcgactg ggcatgatca tccgcgcggg
      841 cgcggtcgat cgcgagatgg tgcagtcgct cggcatcgat atcggccggg tctatcgttt
      901 cgtcttcgca ctgggcgtgg cgctcgcggc cttcgccgga atgattgcgg caccggtcgc
      961 ctcggtctat cccggcatgg gcaaccaggt cctcatcctc tccttcgtcg tcgtggtgat
     1021 cggcggcatc ggctccatca aaggcgcgtt cctgggctcg ctgctgatcg ggcttgccga
     1081 caccttcggc caggtctatg cgcccgagat cgcgggcatg accgtctaca tgctcatggc
     1141 cgcggtgctg ttgtggcggc cggcgggact cttcggacgg gcggtgtagt ggtcggcagc
     1201 gcagagccag gacgtatccc cggtcggcac tggcccgcgc ttctgcttct cttgtgcgtt
     1261 ctcgcgctgg cggcgtttcc ggcggtcgcc aaccgattct atctgcagca catccccacc
     1321 atcatgatca tggcgatctt tgccatgagc ctcgacctgc tggtcgggtt cacggggcag
     1381 gtcagcttcg gccatgccgc gttcttcggg atcggtgcct atgcgctgtt tctggcatcg
     1441 ccggcggagc tggcgccgag cctgtggctg tcgctcgcgc tcgccctcgc cgcggccgcg
     1501 gccgcggcct tcgtcatcgg cctcctggtt ctgcgcagcg ccggcgtcta tttcatcatg
     1561 gcgacactcg ccttcagcca gatgctcttc tactacgccg cccgcgccaa atggctgggc
     1621 ggctcggacg gtgcctatat ctatttgaag cccgacaccg ggctcgcgtt cttcgatctc
     1681 gccgacaagc gtcatttcta ttacctggtc ctggcgctca tggtcgcggt ctacgcgttt
     1741 ttgcgcgtcc tgctgcgctc gcctttcggc catgcgctcc gcgggatcaa ggataacgag
     1801 ccgcgcatgc gctctttggg cttcgccacc tttcgcctga agctcctgag cttcgtcatc
     1861 gccggcacgc tggcgggcct cgcaggatac ctcgatgcgg cgcagttcgg cgtcgtcaac
     1921 cccgatctcc tcggctggcg gctctccggc acggtgctga tgatggtgat actgggcggc
     1981 atgggcacgc tctatggtcc cgtgctcggc gccttcgcgc tggtgctgct gcaggatttt
     2041 ttgagcgacc agacgaagca ttggctgctg cccatggggc tcttcatcat cctggccgtg
     2101 ctggtgctgc ccgacgggct tggcggcctc atcgcctggg caagccgggc gcggcgcagc
     2161 gcggtgcctc gtggctgagc cggtgctcgg cgtcgagggc ctctccaagc atttcggcgg
     2221 cctcgccgcg gtgagcgacg tctcgctcga gctccatcaa ggcgccatcc atgccgtgat
     2281 cgggccgaac ggcgccggca agacgacgct gctcaacctg ctctcgggcg agctcacgcc
     2341 gagcagcggc cgcgtgcgcc atgaaggacg cgacatcgcc ggcctctcgc ccgagcgcat
     2401 cgcccggctc ggcatcgctc gcagctatca gaagagcaac atcttcggcg gcttcaccgt
     2461 cttcgagaac tgccggctcg cagcccaggc cgccttggca cccgggctgc gctgcttccg
     2521 cccggcgcat agctatggcg ccgtcaacgc cgcggcctcg cgtgcgcttg ccgaggtcgg
     2581 cctcgcggga cgcggcgccg ctgtcgccgc gacgctctct cacggcgagc agcgccagct
     2641 cgagctcgcc atggtcctgg cgacggcgcc caaggtgctg ctgctcgacg agccgctcgc
     2701 cggcatgggc gccgaggaga gcgcgcgcat gacggcgctg atcgcgcggc tcgaggcgag
     2761 ccatgccatc ctgctcgtcg aacatgacat ggacgcggtc ttcagcctcg cccggcgcct
     2821 cacggtgatg gtcaatggcc gcgtgctcga gagcgggacg ccggaggcga tccggcgcag
     2881 tccggcagta cgggaagcct atctcggcgc ggcggaggac gtgtcgtgag cgcggcgccg
     2941 ctgctcgagg cgcgggggct tcataccttc tatggctcga gccatgtcat cgacggcgtc
     3001 gacttcacga tcggccgcgg cgagaccgtc gcgctcatgg gccggaacgg gatgggcaag
     3061 acgacgctgc tgcgctcgat gctgggtctg acgccgcccc gcgccggcga ggtgcgggtc
     3121 aagggcgcgc tcatgaccgg cgtcaagccc catcgtatcg cgcgccaggg catcgccttc
     3181 gtgccggaag gacgcggcat cttcgcgacg ctcagcgtgc gcgagcatct cgtcatggcg
     3241 gcgcggcccg gcagcgacgg gcggcgcgat tggacactgc cgcgcgtgct cgagaccttc
     3301 ccgcgtctcg ccgagcggct cgaccatggc ggcacgcagc tttcgggcgg cgagcagcag
     3361 atgctcacca tcgcccgcgc gctcatgacc aatcccgacc tgctgatgct cgacgaggcg
     3421 accgaggggc tggcgccgct catcgcgcgg gagatctggt cgattctcgg ccgcgtccgc
     3481 gccagcggca tcgccgccgt cgtcgtcgac aagaacgtcg ccaagctcgc cgctatcgcc
     3541 gaccgcatcg ttgtcctcgt gaaaggacgc gtcgccttcg ccgggaccgc cgcagcgctc
     3601 aaggcgcaac cggagctgat gcatcgccat ctcggcgttt gattgactca cgtctccgcg
     3661 tcttccagca cggctgcggc ctcatcgagg ccgcggcgac gcgccgcctg tgcaggcgtt
     3721 tggcccaagg gattgcggaa gctcggatcc gcgccgaacg acagcagcag ctcggcaacc
     3781 gcgaccgcct tctcctcgtc atccggcagg cagaagagcg ccggttcgcc ggggcggtcc
     3841 tggcgattgg cccgctccgg ttcctcgccc agcaattcgc gtaagcgctc cacggcgccg
     3901 gcaaagcaaa gcccccggaa gtggcggctg tgcggcgcga ggagctcgat catgtcggcc
     3961 cgctcatgat ggagggcgtg ggtcagaggt gtgccgccat ggcgcagctc gatcgggtcg
     4021 atctcggcac ggtgcgcgag cagcagccgc gcgatctccg gcgcgccgca atgggtggtg
     4081 tagtggaggg cgcgaaagtt cttgtcgtcg ccgatatcgg gcgacatgct gaggtcgagc
     4141 agggcttcgg cgatgtcggc gcggtgccga cggatggcgg cgaacatcgc ttcgtgctgt
     4201 tcgagaagct ccgggtgcgt ccgagccagg cctcgcatgg cggctaaatc gccgccggcg
     4261 gcggcggcga ggaagctttc gcgatcggag agctggggcc tggttgcgcc gtggcgcagc
     4321 agaagttcgg tcacctcctc gcggccggcg agcagagcgt gcttgatgac cggttcgcgc
     4381 gaatagaagt tgatcccgct tgcatccgcg ccgtgctcca ggagccagcg tattcgccgg
     4441 atgtggtcgg gcacggcgtt gccgagaagg taggcgagcg cgctcgtact ccgatcgccg
     4501 ccgagttccc caggtgccgg acccgtccat ttacgagtct cgccgcgttt ctcggattcc
     4561 gcccagagca ggtcgagcca gaaggtggaa tccggcccga gcgacgtatt atagagcgcc
     4621 tgcggtgcga agggatcggc gccgcgcgcg atcagcaagc gcacgaaggc ttccgccgcc
     4681 ggatgcgccg tctgtcccgc ctcgccgccg ccgatgacgc cgaccagcac ggtgaacgcg
     4741 ttggcgccat ccgaccagaa ggcgttggga tcggcgcccg catcgagcag gagggttgcg
     4801 atcgccagcg cgttcgtggc gacggtgttc atgggcaaac gggcataggc gaggcgcagc
     4861 agcggcgtcc acccgtcgaa ggcacagcga tcgctaacga ggccgggatc tttcgagagc
     4921 aaggcgcgga gggcgtccaa gtcgtgcgca gcgacggcgg tgtggatgct ctcgggggcg
     4981 atctcgggat ggcgggcgag aagacgggcg gcaacggcgc cgcgcgcgaa cccgtcgcgc
     5041 tcgtagtcgc cccatttccg tgtcgacgga tcgacgccgt agcggttgac cgacttttca
     5101 aggaagagac ggacgcgatc tgcggcgccg cggccgcgat cctcgatctc ctgtttcagc
     5161 gcggcccagg aagggaaacc gtactggcgc gccagtgcct gctgcatctc acgaagcttc
     5221 gggactccgg cgtggtcggg aaacacccgc cgaaagcgcg cagcggcttc gggatcgcct
     5281 gcggctacgg atttgagcca gcgcttcgcg tcacggcgca gggcgtcgat atttgaattg
     5341 ggcgaaaggg agcggggcat ggaaccttcc ttccgaaact cgcctgatgt ccgcttcgca
     5401 ggctggaaga aggtctacga cgatgtcggt cgatccgagt gggtgggagc aggctccctt
     5461 gccgcggaca ggtggcgccc cgagcgcact gcctggatca aacagccggc gccgccggat
     5521 gtcaacattc gcgcgctccc atcatgagta cacgacataa ccaaaaattg aactcgccgg
     5581 catcgcgcat tcttctagag tgtttgcgtc gcggatgcag atttccctca tcaatctcga
     5641 tcggactccc gaccggctcg cagaattcca ccgccgcaac gagcatctcg aggaggtctc
     5701 acgattccgt gccgtcgacg gccgcacggt cgacgtcggc gcgctcacgc gcatccgcct
     5761 gctccatccg agcgtcgtct cgacctacac caagggcgcc ttgggatgcg ccctctcgca
     5821 tctggcgctc tgggacaggg ccgtgacgcg ggacgaggcc attacgatct gcgaggacga
     5881 cgcgatcttc aaccgcggct tcgtcgaagt ggccgagcgc ctcatcggca cactcgccgc
     5941 cgactgggac gtcatccttt ggggttggaa cttcgattcc gtggtctgtt tcgagatgct
     6001 gcccggcgtc tctccctgct gcctgtcttt cgaccaggac gcccttcgcg ccggcgtcca
     6061 aagcttccag gaccaacgcg tcgcgccgca gctgttcaag ctgcaccggg cgctggggct
     6121 cgtctgctat acgatctcgc ccaagggcgc gcgcgtgctc cgcgaccgct gcctgccgtt
     6181 gcggcagatg gacgtccatt tcccgggcct caaccgcgcg ctctacaaca ccggcatcga
     6241 cattcccatg aacgatgcct atcccgagat cgaagcctat gtcggcttcc cgccgctggt
     6301 cgtcaccaag aacgagcatg agacgaccac ggtccagcgc gactgacgcc gtcttgggcg
     6361 gtcagtcgcg ccgcgtgaac gtgactttaa gtccgtcctt cggtttgggg atcggcgcca
     6421 tctgccattt gggcgcatag cccggcgcca gcgagacatc gaaacgctcg agcagatggc
     6481 gcgcgaagca cttggcctgc atataggcga agtgaaggcc gaggcacata tgcgcgccgg
     6541 cgccgaaggg cgcgaaggcg aatttgtgac ggccgcgcgc attctcctcc gagaaacgca
     6601 gggggtcgaa gcgctgggga tccggccaat gttcggccat gcggtgcgtg aagaggacgc
     6661 tggcattgac gggggtgccg gccgggatgc gataaccggc gtactcgaag tcgcgcatcg
     6721 cccggcgcgg catggagatc accggcggga tgaggcgcat cgcctccttg aaggccatct
     6781 ccgtcaagcg cagctcctcg agccgctcga acggcaaggg ctcggccggc gcgagtccaa
     6841 gcccctgcac ctccgcgcgc agccgctcct gccattccgg gtgagccgcg aggaaataga
     6901 cgaaggccga gagcgacgac gtcaaagtgt catgcgccgc catcatcagg aagctcatat
     6961 ggtcggcgat gtccgcgacg ctcatcagcg acccgtcctc atgggtcgcg cggcagagct
     7021 gcgagaaaag gtcctcgccg ccgcgctcgc ggcgcagcgg cacctgccga ccgaaatatt
     7081 cgacgatgcg cgcccggccc gtgacgccgc gccgcatcgc cgtaaagggc agcggccggc
     7141 ggatgacggc gatggacgcc gcgaccatgt caatgaaggc gcctttgatc gcctcgacct
     7201 cggagcccag cgccgcgccg agaaaggacg cggcggcgag atcgagggtg agctgtttga
     7261 tggcgggata gaagagaaaa tcgcacgggc gctcgcgcca cgcggccagg cggtgggcga
     7321 tgccggcatt gagcgccgcc agataggatt tgagcggccc gaccttgaac gcgaccgaca
     7381 gcgcgcggcg gtggaggcga tggtcgtcga aatcgcgcag catcagcccg cgtgggaaga
     7441 gcaatcccag aaatggcccc cagccctcga gcgacgagaa gagccgcgat tgatcgaaca
     7501 gcacgaggcc gacggcttcg gggcccaaga gcgcgatcga gcggacgccg aacacccggt
     7561 tgcgatagac gagcccgtag cgatcggcca tcgccttcgt ccagccgtaa ggatcggcca
     7621 ggaacctgaa ggtgtcgccc acaagcggcg cgccgtcgct gccggggata tgcgccagat
     7681 ctgcaaggcg tggcgccggc aagtgttgct tggccggtga cgagacgtcg aacgtcgtcg
     7741 aggacatggt cttgcctctt cgctcccagc agcaaagcgc gacgctgcgg tcaagactaa
     7801 ctccgctccg gcccgagcgt ccagccgcgc gacaagctcc ccgccacgtc aacggcccgg
     7861 cggctcggcg tcgatgtcgg tcaagcgacg gtgttgcgga agaacggtcc cccggagatg
     7921 atgccgcgtc atgcagcgag aattcgtgac ggcaggcggc catcgcctcg aatatcagcg
     7981 catcgatggg cgcgccgacg cgccctgtct cgtctttctt cacgaagggc tgggctcggt
     8041 cgcgctctgg cgcgacttcc cggcgaagct cgcgcaagcc gccggcgcgc ccgctgttgt
     8101 ctattcgcgc catggctatg gcgcgtccga ccgcctgacg gcgccgcgcg acgtcgacta
     8161 catgcatcgc gaagcgctgg acacgctgcc ggcgctgcgc gcggccctcg gcctcgggga
     8221 ggtgatcctg atcggccaca gcgatggcgc ctcgatcgcg ctcatccatg cgggaagcgg
     8281 gcgctggccg gtgcgcgccc tcatcctcga ggcgccccat gtcttcgtcg aggacgtctc
     8341 ggtcgagagc atcgccgcgg cgcgcacggc ttacgccgcc acggacctgc gccggcgcct
     8401 cgagcgctat cacggcgacg tcgacggcgc ctttcgcggc tggaacgaca tctggctcgc
     8461 ccccgccttc cgccgctgga acatcgagga atatctgccg ggcgtgcgca cgccggtcct
     8521 ggcgatccag ggcgctgatg acgagtatgg cacgctcgcg cagatcgagg cgatcgaacg
     8581 cggcgtcgcc ggtcctttcg agcggctggt gctcccgcac tgcaggcact cgccccatcg
     8641 cgaccaggaa gacgccgtgc tggcggcgat ggcgggcttc atcgcgcggc tcgcggatca
     8701 ccggtgaaga tcgaaaaagg cagcggaggg cgatcgtgag gaaccgactg gtcgtggtca
     8761 ccggcggatc acgcggcatc ggcgcggcgg tcgcgcggca ggcggccgcc agcggctatg
     8821 cggtgctcgt gaattatcgc gacgacagcg acggcgcgct gcgcgtcgtc cgagagatcc
     8881 gcggcgccgg cgcgacggcc gaggccgtgc gcggcgacgt cggcgtcgag cgcgacgtgc
     8941 tcgcgctctt cgctgcggcc gatcggacca atctcccttt ggccgggctc gtcaacaatg
     9001 ccggcatgac cggcggcttc gcgcggctgc aggacctcgc cgcggagacc ctggcgcgtg
     9061 ttctgacggt gaatgtcgtc ggcgctttcc tgtgcgcgcg cgaagcggcg aaacgcatgt
     9121 cgaccgagcg cggcgggcag ggcggcgcga tcgtgaacat gtcgtcgcgc gcggcccagc
     9181 tcggcggggc cggcgagtgg attcactacg cagcgagcaa gggcgccgtc gatacgctga
     9241 cgatcggcct cgcgaaggag ctgggtcgcg aagggatccg cgtcaacgcc gtcgctccgg
     9301 gtctgattga tacggagctt cacggcgcgg cgggcgatcc cgaccgcgtg cggcgcctcg
     9361 cctccggcgt ccccatggga cggcccgggc ttgcggagga agtggcggag gccgtgctct
     9421 ggctcatctc gccggcggcg gcctatgtca ccggcgcgat cgtgccggtg gccggagggc
     9481 gctaaggctc gggcttctcg ccgggacagt gcgtctccaa cacgcggccg caggcttcga
     9541 tggcggcaat gaagccgtcg gcgaggcg
//
LOCUS       DASYNS010000273         4949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5524341, whole genome shotgun sequence.
ACCESSION   DASYNS010000273 DASYNS010000000
VERSION     DASYNS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5524341"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VGP50_08565"
     CDS             complement(<1..321)
                     /locus_tag="VGP50_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HEV8027470.1"
                     /translation="MLTQPQTRQLWDDPKPTALLVLADGTVLEGLGFGATGHAVGEVC
                     FNTAMTGYEEILTDPSYAGQIITFTFPHIGNVGTNEEDIETVNLAATPGARGVVLHSA
                     ITAPS"
     gene            397..864
                     /locus_tag="VGP50_08570"
     CDS             397..864
                     /locus_tag="VGP50_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEV8027471.1"
                     /translation="MLRDDINNALKAAMKAADKRRVSTLRLINAEILKRETSAAERLT
                     LGDAEILDVMGKMIKQRQESLDIYEKAGRADLAAQEREEIAIISAYLPQHLSDLEAGE
                     AISSLIKELEAATLKDMGRTMTALKERFSGRMDFSKASAMVKRLLAGKGAAAG"
     gene            963..2975
                     /gene="dnaG"
                     /locus_tag="VGP50_08575"
     CDS             963..2975
                     /gene="dnaG"
                     /locus_tag="VGP50_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV8027472.1"
                     /translation="MRFSPQFLDELRARLPVSEVVGRRVKLRKAGREWKGLSPFNKEK
                     TPSFFVNDQKAMWFDFSSGKNGNIFDFVMLTEGVSFPEAVERLAGQAGVAVPVMTREA
                     EEREERRKTLHDIVELAAKFFEATLAARAGARGRGYLADRGIQSSTQLKFRLGYAPGE
                     QYALKEHLGAAKVSVEDMVAAGLLVAGDDIPVPYDRFRDRIMFPIADWRGRVIAFGGR
                     ALEKEVAAKYLNSPETPLFHKGATLYNIAGARQAAHDAAQKLGPSVPPAADNFSARVI
                     AVEGYVDVIAMVSAGFEATVAPLGTALTADQLALMWRMADEPILCFDGDDAGRRAAYR
                     ALDLALPLLKPGKSLRLAALPDGQDPDDLVRSGGREAVNDVLAAARPLADMLWARESG
                     AGPFDTPERRAAFEARIIEVTTAVGDETVRRYYRQDFAARLRRLFAPAEITTGRREWE
                     QRGRRTGAGAGSRLNSPRSRFGGPESYVVASPQLAAGPLHRGHRTAIPRREALILQAA
                     LNHPWLLHDRMEELSEAEFRHAETKKLKAALIDVLAHRSGHEFSHEEEVERAEVIAEL
                     TRQGFADLIARIERAITTPSVWGARPQAGQADVLLTWRQLLALHRQWHSLIRELKDAE
                     LALGEDNSETNYARLQDVKARLSSIDGTEALIEGFGASSGRQSRSL"
     gene            3355..>4949
                     /locus_tag="VGP50_08580"
     CDS             3355..>4949
                     /locus_tag="VGP50_08580"
                     /inference="COORDINATES: protein motif:HMM:NF016433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV8027473.1"
                     /translation="MASKSAGTKTVAIKSKAAPQKEGETPEKEGPETPDAPLPLLDLS
                     DAAVKKMIKQAKKRGYVTHEQLNSVMPAEEVTSEQIEDVLAMMSEMGINVVETEEAEA
                     DEEAREEPEEEETEAGELVEVTPKTPAKSEAKEPAERTDDPVRMYLREMGTVELLSRE
                     GEIAIAKRIEAGREAMIAGLCESPLTFQAVIIWRDELNEGKVFLRDIIDLEATYAGPD
                     AKQVAPGAMPPGDGGAAAAAAGVPGFAPQPPQAVAPPSAPPAATPFKPQAAEAGDGEE
                     ALPEGALGEPDLDEDDLENSMSLAAIEAELKPKVLETFDTIADTFKRLRRLQEIDIQN
                     KLSNEKLSLSPNQERKYRKLKEEIIQEVKSLRLNQARIDALVEQLYDINKRLVSNEGR
                     LMRLSESHGVIREDFLKNYQGFELDPRWLNRVSKLSAKGWKNFIGRDKDRIKELRAQI
                     HALATETGLEIPEFRKIVHMVQKGEREARQAKKEMVEANLRLVISIAKKYTNRGLQFL
                     DLIQEGNIGLMKAVDKFEYRRGY"
BASE COUNT          909 a         1620 c         1672 g          748 t
ORIGIN      
        1 cgacggcgcc gtgatcgccg agtgcagcac cacgccgcgc gcgcccggcg tcgccgcgag
       61 gttgacggtc tcgatgtcct cctcgttggt gccgacgttg ccgatgtggg ggaatgtgaa
      121 ggtgatgatc tggccggcat aggagggatc ggtgaggatc tcctcgtagc cggtcatcgc
      181 ggtattgaag cagacctcgc cgacggcgtg gccggtcgcg ccgaagccga ggccttcgag
      241 cacggtgccg tcggccagga ccaggagcgc ggtcggcttg ggatcgtccc agagttgccg
      301 agtctgcggt tgtgtcagca tcgccgaggg cctacatctc ccgctcaccc gccgcgccac
      361 cgcgcccgcg cggacgaatt gcgagaggcc atagccgtgt tgcgtgacga tatcaacaat
      421 gcgctcaagg cggccatgaa ggcggcggac aagcgccgcg tctcgacgct gcgcctcatc
      481 aacgccgaga tattgaagcg cgagaccagc gccgccgaac ggctgacgct cggcgacgcc
      541 gagatcctcg acgtgatggg caagatgatc aagcagcgcc aggaatcgct cgacatctac
      601 gagaaagccg gccgcgccga tcttgccgcc caagagcgcg aggagatcgc gatcatctcc
      661 gcctatctgc cgcagcacct ctccgacctc gaggccggcg aggcgatctc ctcgctgatc
      721 aaggaactcg aagccgccac gctcaaggac atgggccgca ccatgacggc gctcaaagag
      781 cgcttttccg ggcggatgga tttctccaag gcgagcgcga tggtgaagag gctgctcgca
      841 ggcaaaggcg ctgccgccgg ctgagccgga ctgtggataa ccgggacaat tgctgcgccc
      901 atcattcctg aggcggggcg ccctatcttc gcacctgatc cgcgctacct tgccgaatca
      961 ccatgcgatt ttcgccgcaa ttcctcgacg agctgcgcgc gcggcttccg gtttcggagg
     1021 tcgtgggccg gcgcgtaaaa ttgcgcaagg cgggccggga atggaagggg ctttcgccgt
     1081 tcaacaagga gaagacaccg tcgttcttcg tcaatgacca gaaggcgatg tggttcgact
     1141 tctcctcggg caagaacggc aacatcttcg atttcgtcat gctcaccgaa ggggtgagct
     1201 tccccgaggc ggtcgagcgc ttggccgggc aggcgggtgt cgcggtcccg gtcatgacgc
     1261 gggaggccga ggaacgcgag gaacgccgca agacgctgca cgacattgtc gagctcgccg
     1321 ccaaattctt cgaggcgacg cttgccgcgc gcgccggcgc gcgcgggcgc ggctatctcg
     1381 ccgaccgcgg cattcaatcc tcgacgcagc tcaagttccg gctcggctac gcgcccggcg
     1441 agcaatacgc gctcaaggaa catctcggcg ccgcgaaggt ctcggtcgag gacatggtcg
     1501 cggccgggct cctcgttgcc ggcgacgata tcccggtgcc ctatgaccgc ttccgcgacc
     1561 gcatcatgtt tccgatcgcc gactggcgcg ggcgcgtcat cgcctttggc ggccgcgcgc
     1621 tcgagaaaga ggttgccgct aaatatctca attcgccgga gacgccgctc ttccacaaag
     1681 gcgcgacgct ctacaacatc gccggcgcgc gccaggcggc ccacgatgcc gcgcaaaagc
     1741 tcggcccgtc ggtgccgccc gccgccgaca atttctccgc ccgcgtgatc gcggtcgagg
     1801 gctatgtcga cgtgatcgcc atggtgagcg ccggcttcga ggcgacggtc gcgccgctcg
     1861 gcactgcgct gaccgccgac cagctggcgc tgatgtggcg gatggccgac gagccgatcc
     1921 tgtgcttcga cggcgacgat gccggccggc gcgcggccta tcgcgcgctc gatctcgctc
     1981 tgccgctcct caagccgggc aagagcctgc gcctcgccgc gctgccggac ggccaggacc
     2041 cggacgatct cgttcgttcc ggcggccgcg aggcggtgaa cgatgttctc gccgcggcgc
     2101 ggcccctggc ggacatgctc tgggcgcgcg agagcggggc gggtccgttc gacacccccg
     2161 agcggcgcgc cgccttcgag gcgcgcatca tcgaagtgac gacggccgtc ggcgatgaaa
     2221 ccgtgcgccg ctattaccgc caggattttg ccgcgcggct tcgccggctc ttcgcgcccg
     2281 cggagattac gaccggccgg cgcgagtggg agcagagggg ccgccgcacc ggcgccggcg
     2341 ccggttcacg gctcaattcg cctcgatcaa gattcggcgg ccccgagagc tacgtggtcg
     2401 cgagcccgca actggcggca ggcccgttgc atcgcggcca tcgcaccgcg atcccgcgcc
     2461 gcgaggccct gatcctgcaa gccgcgctca accatccctg gctgctgcac gaccggatgg
     2521 aggagctatc cgaggccgag ttccgccacg ccgagaccaa aaagctcaag gccgcgctga
     2581 tcgatgtcct cgcccatcgc tccggccatg aattcagcca cgaggaggag gtcgagcgtg
     2641 ccgaggtcat cgccgagctc acccggcaag gctttgccga cctgatcgcc cgcatcgagc
     2701 gcgccatcac cacgccttcg gtctgggggg cgcgcccgca ggcggggcaa gccgatgttt
     2761 tgctgacctg gcggcagctt cttgccttgc atcggcaatg gcattcccta attagggaac
     2821 ttaaggacgc ggagttggcg ctcggcgagg acaattccga aacgaactac gcgcggcttc
     2881 aggacgtcaa ggctcggctc tccagcatcg acgggaccga ggccctgatc gaggggtttg
     2941 gcgcctcttc cggccgccag tcgcggtcgc tgtaagcgtt cggcccgcga cccagcgcct
     3001 tatcggtgga cggtccggca gcgaaggaat aagggttcac ggcgccgtcg gcatagttag
     3061 tcggagctta acggagttcc gcggatactg cggccctcga ttcgggggcg gtgccgcgct
     3121 ggcgctgccc tttttggtgg atggccgggt tgagccgacc cctggcgggc cgtttcgtcc
     3181 cgggcgtatc gagtcgccat agggtagggg gacgccgttg tccgtctgcc gggtgtaggc
     3241 gttgccctgg ttgcttagaa ggtaacgggg taaggggtgg ccgtccaacc accccggcgt
     3301 tggaagtgca taaggcaggg acgcggtctg cgacgggcgt tggcggagaa gaacatggca
     3361 agcaagagcg ctggcaccaa gacggtagcg atcaaatcca aggcggcccc gcaaaaagag
     3421 ggcgagacgc cggagaaaga ggggcccgag accccggacg cgccgctgcc gctgctcgac
     3481 ctttccgatg ccgccgtcaa gaagatgatc aagcaggcca agaagcgcgg ctatgtcacc
     3541 catgagcagc tcaactcggt gatgccggcg gaggaggtga cctccgagca gatcgaggac
     3601 gtgctggcga tgatgagcga gatgggcatc aacgtggtgg agaccgagga ggcggaggcc
     3661 gacgaggaag cccgcgagga gcccgaagaa gaggagaccg aagcgggcga actggtcgag
     3721 gtcacgccga agacgccggc aaaatccgaa gcgaaggagc ccgccgagcg caccgacgat
     3781 ccggtgcgga tgtatctgcg cgagatgggc acggtcgagc ttctctcccg cgagggcgag
     3841 atcgcgatcg ccaagcgcat cgaggccggc cgcgaggcga tgatcgccgg gctgtgcgaa
     3901 agcccgctca ccttccaggc ggtcatcatc tggcgcgacg aactgaacga aggaaaagtc
     3961 ttcctgcgcg acatcatcga cctggaagcg acctatgccg gccccgacgc caagcaggtg
     4021 gcgccggggg cgatgccgcc gggcgacggc ggagccgcgg cggccgcagc cggcgtgccc
     4081 ggctttgcgc cgcagccgcc gcaggcggtg gcgccgcctt cggcgccgcc ggcagcgacg
     4141 ccgttcaagc cgcaggcggc cgaggccggc gacggcgagg aggcgctccc cgagggcgcg
     4201 ctcggcgagc ccgacctcga cgaggacgat ctcgagaact cgatgtcgct cgcggccatc
     4261 gaggccgagc tcaagcccaa ggtgctggag accttcgaca ccatcgccga caccttcaag
     4321 cggctccgcc gcctgcaaga aatcgatatc cagaacaagc tgagcaatga aaagctctcg
     4381 ctctcgccca atcaggagcg caaatatcga aagctgaaag aagagatcat ccaggaggtc
     4441 aaatcgctgc gcctcaatca ggcgcgcatc gacgccctgg tcgagcagct ctacgacatc
     4501 aacaagcgcc tggtcagcaa tgagggccgg ctgatgcggc tctccgagag ccacggcgtc
     4561 atccgcgagg acttcctcaa gaattatcag ggcttcgagc tcgacccgcg ctggctcaac
     4621 cgcgtctcca agctctccgc caagggctgg aagaatttca tcggccgcga caaggaccgc
     4681 atcaaggagc tgcgcgcgca gatccacgcg ctggccaccg aaactggcct cgaaatcccc
     4741 gagttccgca agatcgtgca tatggtgcag aagggcgagc gcgaggcccg ccaggcgaag
     4801 aaggaaatgg tcgaggccaa tctccgcctc gtcatctcca tcgccaagaa atacaccaat
     4861 cgcggcctgc agttcctcga cctgatccag gaaggcaata tcggcctgat gaaagcggtc
     4921 gataagttcg aataccgccg cggctacaa
//
LOCUS       DASYNS010000274         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1780999, whole genome shotgun sequence.
ACCESSION   DASYNS010000274 DASYNS010000000
VERSION     DASYNS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780999"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1676
                     /locus_tag="VGP50_08585"
     CDS             <1..1676
                     /locus_tag="VGP50_08585"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8027474.1"
                     /translation="KRHRTAEAAACYREVLRLRPEDAQACNALGELFANATRCEEAVD
                     CFRAAVRLEPNFADAWNSLALMLSILGRQDEAVACYEELIRLKPDSLRPKVNRCIARL
                     PIIYRSDAEVESARARFAAELEEICRQPARQVSLEAITGLPPFYLAYQGRSDRDLQAR
                     YGRFVAEVMAAHYPAWTTPPEVTPPGPGEPIRVGFLSSFFYSHSNWKIPLKGWLSGLD
                     KRRFQIFAYHLDHTADQETAVARELCHRFVHDLPPMLDPWAKAIRADRLHVLVIPGIG
                     LDALTHRLAALRLAPVQVASWGHPDTTGLPTIDHYLSSALMEPADGDEHYTEQLIRLP
                     NLSIAYEPLALAPVSVSRAEIGVPDDAIFYWCCQSLFKYLPRYDWVFPRIAAAVPAAR
                     FVFIEHNRGDTVTQIFRERLAAAFAAAGLDAARHCVFLPPMDMARFAAIGRRADLFLD
                     SLGWSGCNSTLEALGADLPVVTMAGDLMRGRHSAAILTMLGMPELVADTPEAFAALAI
                     TLGRDPEARQALRARIARDKHRLYGDQAAVEGLSRYLEDAASGALRGRP"
     gene            1690..>2037
                     /locus_tag="VGP50_08590"
     CDS             1690..>2037
                     /locus_tag="VGP50_08590"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8027475.1"
                     /translation="MAEPGDGQSFAPRNAAAAASAGGTASPADIAALFETGIGHHMAG
                     RLEQAQVIYRDILAVDPGNADCRFRMGLLAAQAGRPDLAIQHLRETIARGPEAATYHL
                     HLGMILSGLGRLDE"
BASE COUNT          298 a          755 c          646 g          338 t
ORIGIN      
        1 cgaagcgcca tcggacggcg gaggcggcgg cctgctatcg cgaagtcttg cgcctcaggc
       61 ccgaggatgc acaggcctgc aacgccttgg gcgagctgtt cgcgaatgcc acgcgctgcg
      121 aggaagccgt cgattgcttt cgcgcagcgg tgaggctcga gcccaatttt gccgacgcct
      181 ggaacagtct ggcgctgatg ctgagcattc taggacgcca ggacgaagcc gtcgcctgct
      241 atgaagagct gatccgcctc aagccggact cgctgcggcc caaggtcaac cgctgcatcg
      301 cccgcctccc gatcatctat cgcagcgacg ccgaggtgga gagcgcccgc gcccgcttcg
      361 ccgcggagct cgaggagatc tgccgccagc ccgcccggca ggtgagtctc gaggcgatca
      421 ccggccttcc ccccttctat ctcgcctatc aggggcgctc ggaccgcgat ctccaggcgc
      481 gctacggccg cttcgtcgcc gaggtcatgg ccgcccacta cccggcctgg accacgccgc
      541 cggaggtaac gccgccgggg cccggcgagc cgatccgggt cggttttctg tcgtcgttct
      601 tctacagcca ctccaactgg aagatcccgc tcaagggttg gctttccggc ctggacaagc
      661 gccgcttcca gatcttcgcc taccacctcg accacacggc ggaccaggaa accgccgtcg
      721 cgagagagct ctgccaccgc ttcgtccacg acttgccgcc gatgctcgat ccctgggcca
      781 aggccatccg ggccgatcgc ctccatgtcc tggtgatccc cggcatcggg ctcgacgccc
      841 tcacccaccg gctcgcggcg ctcaggcttg cgccggtgca ggtggcgtcc tggggacatc
      901 cggacacgac cggcctgccg accatcgatc attacctcag cagcgcgctg atggagccgg
      961 ccgacggcga cgagcactat acggagcagc tgatccggct gcccaatctc tcgatcgcct
     1021 acgagccgct ggcgctggcg cccgtctccg tgtcgagggc cgagatcggc gtcccggacg
     1081 acgcgatctt ctattggtgc tgccagtcct tgttcaagta tctgccgcgc tacgactggg
     1141 tctttccccg gatcgccgct gccgttccgg ccgcgcgctt cgtcttcatc gagcacaacc
     1201 gcggcgatac ggtgacgcag atcttccgcg agcgcctcgc tgcggccttt gcggctgccg
     1261 gcctcgatgc cgcccggcat tgcgtcttcc tgccgccgat ggacatggcc cgctttgccg
     1321 ccatcggccg tcgcgccgac ctgtttctcg acagcctcgg ctggtccggc tgcaactcga
     1381 cgctggaagc gctgggcgcc gacctgccgg tcgtcaccat ggccggcgac ctcatgcgcg
     1441 gccggcacag cgcggcgatc ctgacgatgc tgggcatgcc ggagctcgtc gccgacacgc
     1501 cggaggcctt cgcggccctg gcgatcaccc tcggacgcga ccctgaggca cgccaggcgc
     1561 ttcgcgcacg gatcgctcgc gacaagcatc gcctctacgg cgatcaggcg gcggtcgaag
     1621 gcctttcccg ctatctcgag gacgccgcca gcggcgcgct ccgtggacgg ccctagtctg
     1681 ccacgcccga tggccgagcc cggcgacggt caatccttcg cgccgcggaa cgcggccgcc
     1741 gccgcgagcg caggcggcac ggcctcgccg gccgacatcg ctgcattgtt cgagacgggt
     1801 atcggccacc atatggcggg gcgtcttgag caggcgcagg tgatttatcg tgacattctt
     1861 gccgtcgatc ccggcaacgc cgactgtcgc ttcagaatgg gattgctcgc ggcgcaagcc
     1921 ggacgcccgg atctcgcgat ccagcacctc agagagacga tcgcgcgcgg ccccgaggcg
     1981 gccacctacc accttcatct cggcatgatc ctgagcggcc tcggccgcct cgacgag
//
LOCUS       DASYNS010000275         2998 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2092486, whole genome shotgun sequence.
ACCESSION   DASYNS010000275 DASYNS010000000
VERSION     DASYNS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2092486"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..742)
                     /locus_tag="VGP50_08595"
     CDS             complement(29..742)
                     /locus_tag="VGP50_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV8027476.1"
                     /translation="MKRIAAAAALILFACAACSGGGATAPDDSAPAQTEYLPPAGKGR
                     VVVVLSGHSGPYQYKPIAAEVAKLGYYTVLLDGKDILDADQHGGERLKAAILRAQQTP
                     NALPGKVAVIGFSQGGGGALTYATRLPDLVAIVVAWYPQTNFITRYTDYKTYVGRMKV
                     PTLILAGEKDNYHDCCLIESMRVLEASARELGKPLDLVVYPEANHGFNLPAGLGYRAA
                     DTADAWGRSTALLRQYLGG"
     gene            complement(834..2510)
                     /locus_tag="VGP50_08600"
     CDS             complement(834..2510)
                     /locus_tag="VGP50_08600"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV8027477.1"
                     /translation="MMVRPLDSFIEQRRDQLYPKLDPLEIERMRRFGELRSFAAGEHL
                     VTVGDVASGLAVILSGRVAVSDRDPLGEPRLIITHREGNFMGELAQLTGRPALVDVVA
                     EGPVEALVIPPERLRALMIAEADLGERIMRALILRRMRILQTGTGATVIIGPARNGDV
                     LRLQEFLRRSGQPQQLLDPETDPSAKALTERFRVDPGRLPIVLCPGGQLLRNPGQDEL
                     ARCLGLVRPIDPDRLYDVVVVGAGPAGLATAVYAASEGLAVLVLDCRAFGGQAGASAR
                     IENYLGFPTGITGMALMARAWNQAQKFGAEMAIPDEVRSLAAPDGAGGPFTLTLHTDE
                     RVRARSVVVATGARYRRLDVANLEAFEATSVHYWASPIEGKLCANQDVALVGAGNSAG
                     QAAVYLASQGAKVSMLARRDLGATMSRYLVERISGLANVEVVAGATVSGLEGEDGALK
                     AVRWRLVATGEEIRRPLGHLFLFIGADPNTDWLGGSGVALDAKGFILTGDQAGAGRHA
                     LETAIPGVFAIGDVRARSVKRVAAAVGEGAQVVAALHAFLTERVSAQPQS"
     gene            2670..>2998
                     /locus_tag="VGP50_08605"
     CDS             2670..>2998
                     /locus_tag="VGP50_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV8027478.1"
                     /translation="MSENVRVEIADGVCTVTIARPEKKNSLTQAMYAGLADAIERAES
                     ERSVGCLLLTGAGGVFCAGNDIADFRAPRPAESRDAPSPSRRFYFGLTNFAKPIVAAV
                     PGLAIGIG"
BASE COUNT          492 a         1015 c         1028 g          463 t
ORIGIN      
        1 tccgcgccct ccgcgctcct ctttcttttc acccgccgag atactgccgc agcagcgcgg
       61 tgctgcggcc ccaggcatcg gcggtgtcgg cggcgcgata gcccaggccg gccggcagat
      121 tgaagccgtg attggcttcg ggatagacca ccagatcgag cggcttcccc agctcccgtg
      181 ccgatgcttc gaggacgcgc atgctctcga tgaggcagca gtcgtggtag ttgtccttct
      241 cgccggcaag gatgagcgtc ggcactttca tccgcccgac ataggtcttg tagtcggtgt
      301 agcgcgtgat gaagttggtc tgcggatacc acgcgaccac gatggcgacg agatccggca
      361 ggcgcgtcgc ataggtgagg gcaccgccgc ccccttgcga gaagccgatc actgcaacct
      421 tgcccggaag cgcgttcggt gtctgctgcg cacgcaggat cgcggccttg agcctctcgc
      481 cgccatgctg gtccgcgtcg agaatgtcct tgccgtccag cagcaccgtg taatagccga
      541 gcttcgccac ctctgccgca atgggcttgt actgataggg tccgctgtga cccgagagaa
      601 cgacgacgac ccgtcccttg cccgccggcg gcagatactc cgtctgcgcc ggagccgaat
      661 cgtccggcgc ggtggcaccg ccgccgctac aggcggcgca ggcgaacagg atgagagccg
      721 ccgcggcggc aatgcgcttc atcggaacat cacccctcgg tgacgaattt gacggcgggg
      781 caggccgggg tcaagcgacg taccggccat cacgaccatt tagagaacgg tcttcaactt
      841 tgcggctgcg cgctgactct ctccgtcaag aaggcatgca gtgccgcaac cacctgcgcg
      901 ccctcgccga ccgcggcggc gacgcgcttc accgatcttg cgcgcacatc gccgatggcg
      961 aagacgccgg gaatcgccgt ttccaaggca tggcgcccgg cgccggcctg atcgccggtg
     1021 aggatgaagc ccttggcgtc gagagcgacg ccggatcctc cgagccagtc ggtgttgggg
     1081 tcggcgccga tgaagagaaa gagatgcccg agcggccgcc ttatctcctc gcccgtcgcg
     1141 acgagccgcc agcgcacggc cttgagcgcg ccatcctcgc cttcgaggcc gctgaccgtg
     1201 gcgcccgcca ccacttccac attggcgaga ccgctgatgc gctcgaccag ataacgcgac
     1261 atcgtcgcgc caaggtcgcg gcgcgccagc atcgagacct tggcaccctg gctcgccaga
     1321 tatacggccg cctggccggc cgagttgccg gcacccacca gcgccacgtc ctgattggca
     1381 cagagcttcc cttcgatcgg cgatgcccag taatgcacgc tcgtcgcctc gaaggcttcg
     1441 aggttggcaa catcgagccg ccgatagcgc gccccggtgg cgacgaccac ggagcgggcg
     1501 cgcacgcgct catctgtgtg aagggtcaag gtgaaaggac cgccggcgcc gtcgggcgcg
     1561 gcgaggctcc tcacctcgtc cgggatcgcc atctcggcgc cgaatttctg cgcctgattc
     1621 caggcgcgcg ccatcagcgc catgccggtg atgccggtgg ggaagcccag atagttctcg
     1681 atcctggcgg aggcgccggc ctggccgcca aaggcgcggc aatcgaggac gaggacggcg
     1741 aggccttccg atgccgcata gacggccgtg gcaaggccgg ccggcccggc gccgacgacg
     1801 acgacgtcat agaggcggtc cggatcgatg ggccgcacca ggccgaggca gcgcgccagc
     1861 tcgtcctggc cgggattgcg cagcaattgt ccgccgggac acagcacgat ggggagtcgt
     1921 ccgggatcga cacggaagcg ctcggtcagc gctttggccg aggggtcggt ctcgggatcg
     1981 agcaattgct ggggctggcc gctgcggcgg aggaactcct gcagccggag cacgtcgccg
     2041 ttccgcgcgg gaccgatgat gaccgtggcg cccgttccgg tttgcaggat gcgcatgcgg
     2101 cgcaggatca gcgcgcgcat gatgcgctcg cccagatcgg cctccgcgat catgagcgcg
     2161 cggagccgct ccggcggtat gacgagcgcc tcgaccggcc cctccgcgac cacatccacc
     2221 agggccggac gtcccgtcaa ctgcgcgagc tcgcccatga agttgccttc gcggtgggtg
     2281 atgatgagcc tgggctcgcc cagaggatcg cggtcgctga cggcgacccg gccggagagg
     2341 atgaccgcaa gacccgacgc gacgtcgccc acggtgacga gatgctcgcc cgccgcaaag
     2401 gagcgaagct cgccgaagcg ccgcatccgc tcgatctcga gcggatcgag cttgggatag
     2461 agctggtcgc gccgctgctc gatgaaggag tccaaaggcc gcaccatcat gcatgctgca
     2521 ctgcagcggt taggtggggc attctgcggt gatgcgcaag gcgttcggca acggccggcg
     2581 gttcagcacc tgcaggggga tgagctattt ctcaccttgc ggcggcccgt gtactctcgc
     2641 cgccgggaag gcgaggaggg gagatcgaaa tgagcgagaa tgttcgagtc gagatcgcgg
     2701 acggcgtctg caccgtgacc atcgcgcggc ccgagaagaa gaacagcctg acccaggcga
     2761 tgtatgcggg tctcgccgac gccatcgagc gggccgagag cgagcggagc gtcggctgcc
     2821 tgctgctgac cggcgccggc ggcgtcttct gcgccggaaa cgacatcgcc gatttccggg
     2881 cgccgcgccc ggcggagtcg cgggatgccc cgtcgcccag ccggcgcttc tatttcgggc
     2941 tcacgaattt cgcgaagccg atcgtcgccg ccgtgccggg cctcgccatc ggcatcgg
//
LOCUS       DASYNS010000276         8831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3965700, whole genome shotgun sequence.
ACCESSION   DASYNS010000276 DASYNS010000000
VERSION     DASYNS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8831
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3965700"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VGP50_08610"
     CDS             <1..293
                     /locus_tag="VGP50_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8027479.1"
                     /translation="DGLHGCFIALTVTDTGSGMSPEVLARAFEPYFTTKPDGRGTGLG
                     LSTVRDFAKRVRGAVAIESALGRGTTVTVYLPRGSSSKEGVGNKPAESDGDR"
     gene            488..1711
                     /locus_tag="VGP50_08615"
     CDS             488..1711
                     /locus_tag="VGP50_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV8027480.1"
                     /translation="MASIGIRWRIYGAFGVLMILGMAIAGLGAANLPMYVELVAQALA
                     FLAGAIIAISVGRSIVLPIAAMTEAMAKIAAGHSEVQIPSRDSSDEIGAMAQAVEVFK
                     RNTGEHARLEQSEKQRQTQAAQERQRAMAALAESFERSIGQIVNTVASSATEMQVSAQ
                     SMASTAEEASRQAAAVSAASTAAHGNARAVAEEGEQLTLSIVEIGQRVERSSLIAAKA
                     VEDARRTDATVEGLTTAADKIGAVVNLIQNIAGQTNLLALNATIEAARAGAAGKGFAV
                     VASEVKTLANQTAKATGDIATQVAAIQNVTGEAVSAIRVIGATIDHMNDISMEIAAAV
                     EEQSVVARDIAGHVRQVADRTEEVSGNISGMSNASTDVGRAATQVLNASQALARLSER
                     LRAEVDGFLAGIRAA"
     gene            1920..3230
                     /locus_tag="VGP50_08620"
     CDS             1920..3230
                     /locus_tag="VGP50_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV8027481.1"
                     /translation="MLWLQIAFIFLLIVLNGFFAMAEMAVVSARKARLQHAADLGRDG
                     ARVALELKRDPGRFLSTVQIGITVISVLASVFGGATLADTFTQYLENLPDPIGRYAKS
                     ISFAVVVILIAYFTLILGELVPKRLALGRPEYIAARLSRLLLAMSRLATPVERLLSAT
                     SNLVLRLLPVRPGEPPPVTEEEITLMMREATAAGHFQAAETAMVEMVFRLGDRRVSTV
                     MTPRTQVEWLDLTDSEEENRRKLHDSAYSRFPVVDGGPQQVVGIVQVKDLLTAALSGR
                     PFDLRAVVKAPIYVPSTVTALRALEIFKKSGAPMALVVDEYGDFEGVVTLNDILQSLV
                     GDIADPGDAGAQAVIKRDDGSWLVDGMVTIDAIKDLIGAPNLPGEDSGDFQTLGGFMM
                     ARINRVPMVGDRIIVKGFRFEVVNMDGRRVERVLIAPPKAVTRR"
     gene            3359..4156
                     /locus_tag="VGP50_08625"
     CDS             3359..4156
                     /locus_tag="VGP50_08625"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HEV8027482.1"
                     /translation="MADTFPTFFVSHGAPTLALDPGATGAFWQRLAAELPRPEAVLCI
                     SAHWTTAEPEVSAAAQNDTIHDFYGFPEPLYRIAYPAPGAPALAARVAELLGGGGAAT
                     RIDRARGLDHGAWVPLRSMYPEADVPALQLSVQPQLDAAWHRRLGAALAPLRSEGVLI
                     LASGGAVHNLRALDWSGEGPAPAWARAFDDWLAATLAQGNTAALLDWRNAAPDARQAQ
                     PTTEHFVPLFVALGAAGEGARGERLHQGFTYGSLSMAAFRFARGEGA"
     gene            complement(4467..4805)
                     /gene="grxD"
                     /locus_tag="VGP50_08630"
     CDS             complement(4467..4805)
                     /gene="grxD"
                     /locus_tag="VGP50_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEV8027483.1"
                     /translation="MSVNNTVTDRIKQDIADNAVLLYMKGTPVFPQCGFSAAVVQILT
                     HLGVKFKGVDVLADPEIRQGVKEFSSWPTIPQLYVKGEFVGGCDIVREMFESGELGEL
                     LKDKGVTRAA"
     gene            complement(4836..5072)
                     /locus_tag="VGP50_08635"
     CDS             complement(4836..5072)
                     /locus_tag="VGP50_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEV8027484.1"
                     /translation="MAMDAATIERLIRQSLPDAQVTIEDLRGDGDHYAAHIVSTAFKG
                     KTRVQQHQMVYQALGGRMGGELHALAIQTSAPED"
     gene            complement(5096..7300)
                     /gene="purL"
                     /locus_tag="VGP50_08640"
     CDS             complement(5096..7300)
                     /gene="purL"
                     /locus_tag="VGP50_08640"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014416306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEV8027485.1"
                     /translation="MTGPGAPPKIDPDIVAQHGLTPEEFARAVKIMEREPNLTELGIF
                     SVMWSEHCSYKSSRKWLKRLPTTGPRVICGPGENAGVIDIGDGQAAIFKMESHNHPSF
                     IEPYQGAATGVGGILRDVFTMGARPIANLNALRFGSIDHPKTRHLLAGVVAGIGGYGN
                     CVGVPTVGGECNFHPSYNGNILVNAMTVGVADARRIFYSAAAGPGNPVIYVGAKTGRD
                     GIHGATMASAEFNEDAEAKRPTVQVGDPFTEKLLIEACLELMAGDAIVAIQDMGAAGL
                     TSSSVEMAGKGGLGIELDLDRVPVREDGMTAYEIMLSESQERMLMILKPGSEDEARRI
                     FEKWELDFAIIGRVTETGRLVLRRDGVVAANMPVGPLVTEAPLYDRPWIETPPQRDVH
                     PTELRVEMDVLQLLKMLLGTPDLSSKRWIWEQYDHLVMGHTVQRPGGDAAVVRIPGHQ
                     KALALVTDCTPRYCAADPRRGGAQAVAETWRNLTAVGATPLAITDNMNFGNPERPEIM
                     GQFVGCIEGMREACLALDYPVVSGNVSLYNETNGKGILPTPVIGGVGLIEDAATSVTL
                     ALKRPGEAILLIGETKGHVGSSLFLRAVTGADSGAPPPLDLAAERRNGDAVRAAILGG
                     AVSACHDVSDGGILVAIAEMAMAGNLGATVAAPNGASPYAFWFGEDQSRYVLTTNDPA
                     SVTGRLTAAGVPVARLGTTGGPSLTVEGVGAISTAELRRINEAWLPGYMATP"
     gene            complement(7297..7986)
                     /gene="purQ"
                     /locus_tag="VGP50_08645"
     CDS             complement(7297..7986)
                     /gene="purQ"
                     /locus_tag="VGP50_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEV8027486.1"
                     /translation="MQAAIIIFPGSNREQDVHDALARAAGRAPLKVWHRDSALPKADL
                     IVIPGGFAYGDYLRCGAMAAHSPIMRDVKAAAARGTPVLGICNGFQILTEAGMLPGVL
                     LANKTLKFHCKNVDLRVETSQSIFTCRYETGQVIRAPVAHHDGNYFADAETLDRLEDK
                     GLVAFRYCTPEGEVSEAGNANGSARNIAGIFNESKTVLGLMPHPEDATDPLLGGTDGC
                     ALFDGLVEALA"
     gene            complement(8186..8395)
                     /locus_tag="VGP50_08650"
     CDS             complement(8186..8395)
                     /locus_tag="VGP50_08650"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEV8027487.1"
                     /translation="MASPKQRRKVKRVMREYKEGELKSGGRRKVKSRRQAIAIAMSES
                     GQSRNRRGRKKAANGRRKPARRKKQ"
     gene            complement(8553..8795)
                     /gene="purS"
                     /locus_tag="VGP50_08655"
     CDS             complement(8553..8795)
                     /gene="purS"
                     /locus_tag="VGP50_08655"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEV8027488.1"
                     /translation="MKARVHVMLKNGVLDPQGKAIGHALASLGFAGVNEVRQGKVIDL
                     DLAEADATRARAAVEQMCQKLLANTVIESYQIELRD"
BASE COUNT         1422 a         2924 c         2960 g         1525 t
ORIGIN      
        1 atgacgggct tcatggctgc ttcattgcat tgaccgtgac cgacacgggc tcagggatgt
       61 cgcccgaggt gctcgctcgc gccttcgagc cctacttcac cacgaagccc gacggcagag
      121 gcacgggtct cgggctgtcg acggtgcgcg acttcgccaa gcgtgtccgc ggcgccgtgg
      181 cgattgagag cgcgctcgga agaggcacca ccgtcacggt ctacctgccg cgcggctcat
      241 cctcgaaaga aggcgtcggc aacaaaccgg ccgagagcga cggcgatcga tgagccttcg
      301 gttccctgcg cgagcttcgg ggtttgctat tttccgttct gggcggcgtc ttgcggcgaa
      361 accgctgctg gccgcccgcg atgacgcttc gcgtcgcgcg tattaaatca aaattttagc
      421 attcccctgc taggatcgcg tccgcgccga cactcctcgg tgattcgctc cctacaggga
      481 tgtgtccatg gcaagcatcg gaattcgttg gcgaatctat ggcgcgttcg gcgtcctgat
      541 gatcctcggc atggccatcg cgggtctggg cgcggccaac ctgcccatgt atgtcgagct
      601 ggtcgcccaa gcgctggcct tcctggcggg cgcgatcatc gcgatatccg tcgggcgcag
      661 catcgtcctg ccgatcgccg ccatgacgga ggccatggcg aagatcgccg ccggccacag
      721 cgaggtccaa atcccgtccc gcgacagcag cgacgaaatc ggcgcaatgg cgcaagcggt
      781 cgaggtgttc aagcgcaaca ccggcgagca cgcgcgcctc gagcagtccg agaagcaacg
      841 ccaaacgcag gcggcgcaag agcggcagcg cgccatggcg gcgctggcgg agagcttcga
      901 gcgcagcatc ggacagatcg tcaacacggt ggcttcgtcg gcgaccgaga tgcaagtctc
      961 ggcgcaatcc atggcgagca cggccgagga agcgtcgcgt caggcggcgg ccgtctccgc
     1021 ggcgagcacg gccgcccatg gcaacgcgcg cgccgtggcc gaggagggcg agcagctcac
     1081 cctctcgatc gtcgagatcg gacaacgcgt cgagcgctcg tccctgatcg cggccaaagc
     1141 ggtcgaggat gcgcggcgga cggacgccac cgtcgaaggc ctcaccacgg ccgccgacaa
     1201 gatcggcgcg gtggtgaacc tcattcagaa catcgccggc cagaccaatc tgctcgcgct
     1261 caacgcaacg atcgaggcgg cgcgtgccgg cgcggcgggc aagggcttcg cggtagtcgc
     1321 ctccgaagtg aaaaccctcg ccaaccagac ggcgaaggcg accggcgaca tcgccacgca
     1381 ggtcgccgcc atccagaacg tgaccggcga ggcggtgtcg gcgatccgcg tcatcggcgc
     1441 gaccatcgat cacatgaacg acatatcgat ggagatcgcg gccgccgtcg aggagcaaag
     1501 cgtcgtggcg cgcgacatcg ccggccatgt gcgccaggtc gccgacagga ccgaagaggt
     1561 ctcgggcaac atttccggca tgtccaacgc ctcgaccgat gtcggcaggg ccgcgaccca
     1621 ggtgctgaac gcgagccagg cgctcgcgcg cctctccgag cggctgcgcg ccgaggtcga
     1681 cggttttctc gccggaatcc gcgcggcctg agccgaccac ttcagcatcg gcgtgtccat
     1741 cgatcctctc tcccctcggg gagagggtta gaatgaaggg gctacagagg gatcgtgccg
     1801 gcggccccct caccccgacc ctctccccaa gggggagagg gggaaaggcg cgccgggcct
     1861 gcgcaggcgg cgcattgcca ctaaactagg gccgcgttcg ccaaccgagc gggtccgcca
     1921 tgctgtggct gcagattgcc ttcatctttc tcctcatcgt cttgaacggc ttcttcgcca
     1981 tggcggagat ggccgtcgtc tcggcgcgca aggcgcggct acagcatgcc gccgatctcg
     2041 gccgcgacgg cgcgcgggtg gcgctcgagc tcaaacgcga tcccggccgg ttcctctcga
     2101 cagtgcagat cggcatcacc gtgatcagcg tgctggcgag cgtgttcggc ggcgccacgc
     2161 tggccgacac gttcacgcag tatctggaga atcttcccga tccgatcggc cgttacgcga
     2221 agtcgatcag cttcgccgtc gtggtgatcc tgatcgccta tttcacgttg atcctgggcg
     2281 aactggtgcc gaagcgcctg gcgctgggcc ggcccgaata catcgcggcg cggctgtcgc
     2341 gcctgcttct ggcgatgtcg cggctggcga cgccggtcga gcggctgctg agcgccacca
     2401 gcaacctcgt gctgcgcctg ctgccggtgc ggcccggcga gccgccgcct gtcaccgaag
     2461 aagagatcac gctgatgatg cgcgaggcca cggctgccgg ccattttcag gcggcggaga
     2521 cggccatggt ggagatggtg ttccgcctgg gcgatcgccg cgtcagcacg gtgatgacgc
     2581 cgcgcacaca agtcgagtgg ctcgacctca ccgacagcga ggaggagaac cgccgcaagc
     2641 tccatgacag cgcctattcg cgctttcccg tcgtcgatgg cgggccgcag caggtggttg
     2701 gcatcgtcca ggtgaaggat ctgctgaccg cggcgctctc gggccggccc ttcgatctcc
     2761 gcgccgtcgt gaaggcgccg atctatgtgc cgagcacggt cacggcgctg cgcgccctcg
     2821 agatcttcaa gaagtccggc gcgcccatgg cgctggtcgt ggatgaatac ggcgatttcg
     2881 aaggcgtcgt gacactgaac gacatcctcc agtcgctggt cggcgatatc gccgatcccg
     2941 gcgacgccgg cgcgcaggcc gtgatcaaac gcgatgacgg gtcatggctg gtggacggca
     3001 tggtgacgat cgacgcgatc aaggacctga tcggggcgcc aaatcttccg ggcgaagaca
     3061 gcggcgattt ccagacgctt ggcggcttca tgatggcgcg catcaaccgc gtgccgatgg
     3121 tgggcgaccg catcatcgtc aagggcttcc gcttcgaggt ggtgaacatg gatggccgcc
     3181 gcgtcgagcg cgtcctgatc gcgcccccga aggcggtgac gcggcgctga gaaagttgtc
     3241 agtcgtcagt cgtcagtcgt cagtcgtcag tggtcagttc tcagtcgatg acagactgaa
     3301 aactgataac tgataacttc ggttgcggcg cggcgacctc ggccccactt aagtcggcat
     3361 ggccgacact ttccccacat tcttcgtgtc ccatggggcg ccgacgctgg cgctggatcc
     3421 cggagcgacc ggcgccttct ggcagcggct tgccgccgag ttgccgcggc ccgaggcagt
     3481 gctgtgcatc tccgcccatt ggacgacagc agagcccgag gtgagcgccg ccgcacagaa
     3541 cgacaccatc catgacttct acggctttcc cgagccgctc tatcgcatcg cctatccggc
     3601 gccgggcgca cccgcgctcg cggcgcgcgt tgccgagctg ctcggtgggg gcggcgcggc
     3661 gactcggatc gatcgcgcgc gcgggctcga tcatggcgcc tgggttccgt tgcggtccat
     3721 gtatcccgag gcggatgtgc cggcactcca gctctcggtg cagccgcagc ttgacgccgc
     3781 ctggcatcgt cgattgggtg cggcgctggc gccgctccgg tccgaaggcg tgctgatcct
     3841 cgccagcggc ggcgcagtgc acaatcttcg cgcgctcgac tggagcgggg agggtcctgc
     3901 gccggcctgg gcgcgggcgt tcgacgattg gctcgcggcg acgctcgctc aaggcaatac
     3961 tgcggcgctg ctcgattggc ggaacgccgc ccccgacgcg cgccaggcgc agccgacgac
     4021 cgagcatttc gtgccgcttt tcgtggcgct gggcgccgcc ggcgagggcg cccgcggcga
     4081 gcgcctccat cagggcttca cctatggcag cctgagcatg gcggcgttca ggttcgcgcg
     4141 cggcgaaggc gcgtagcgct ttcgtccgcg gcgggcggca gcgattgcgc agcaatcgcg
     4201 agagcccgca cggagcagaa ctaggtcgaa tggacattcg cagtgatgcg cgtctctgcc
     4261 cctctcccgc atcgcgggag agggtggtgc gcgcaaagcg cgcgccgggt gagggggcgg
     4321 ttcaagcaaa ggcgctttgc ctgagccgtc ccctcaccct cccactgctt cgctgaagct
     4381 tcgcagtggg cccctccctc tcccgcgatg cgggcgaggg gatgttaacc ccggcttggt
     4441 gcggacgaac gcgcgaagcg cattcgctag gcggcgcgcg tgacgccctt gtccttgagg
     4501 agctcgccga gctcgcccga ttcgaacatc tcgcggacga tatcgcagcc gccgacgaac
     4561 tcgcccttca catagagctg cgggatggtc ggccagctcg agaattcctt gacgccctgg
     4621 cggatctccg gatcggccag cacgtcgacg cctttgaact tcacgccgag atgcgtgagg
     4681 atctgcacca cggccgccga gaagccgcat tgcggaaata ccggcgtgcc tttcatgtag
     4741 agcaggacgg cattgtccgc gatgtcctgc ttgatccggt ccgtcaccgt gttgttgacg
     4801 ctcatgggga aatttccttc gcgatgaagc tcgatctaat cttcgggggc gctcgtctgg
     4861 atcgcgagag cgtgcagctc gccgcccatg cggccaccca gcgcctggta aaccatctga
     4921 tgctgctgca cccgtgtctt gcccttgaag gcggtcgaga cgatatgggc cgcataatgg
     4981 tcgccgtcac cgcgcagatc ctcgatggtg acttgcgcgt ccggcaagct ctgccggatc
     5041 agccgctcga tggtcgccgc atccatggcc atgcgcgtgt ctccggtcgc gatccctaag
     5101 gcgtcgccat gtagccgggc agccacgcct cgttgatccg ccgcaactct gcggtggata
     5161 tggcaccgac tccctcgact gtcaacgaag gcccgcccgt cgtgcccaga cgcgcgaccg
     5221 gcacaccggc tgcggtcaac cggcccgtca cgctcgccgg atcgttcgtt gtgaggacgt
     5281 aacgactctg gtcctcgccg aaccagaaag cgtaaggaga agcgccgttc ggcgccgcca
     5341 ccgtcgcgcc cagattgccg gccatggcca tctccgcgat agccacgagg atgccgccgt
     5401 cggaaacatc atggcaggcg ctcaccgcgc ccccgaggat ggcggcgcgg accgcatcgc
     5461 cgttgcgccg ctcggcggcg agatcgagcg gtggcggcgc gccgctatcg gcgccggtca
     5521 ccgcgcgcag gaacagcgag ctgccgacat gccccttcgt ctcgccgatg agcagaatgg
     5581 cctcgcccgg gcgttttaaa gccaaagtga ccgatgtcgc ggcgtcctcg atcagcccga
     5641 cgccgccgat cacgggcgtc ggcaggatgc ccttgccgtt ggtttcgttg taaagcgaga
     5701 cattgccgga gacgacgggg tagtcgagcg ccaggcaggc ctcgcgcatg ccctcgatgc
     5761 agccgacaaa ttggcccatg atctcgggac gctcgggatt gccgaaattc atattgtcgg
     5821 tgatggcgag cggcgtggcg cccacggcgg tgagattgcg ccaggtttcc gccacggcct
     5881 gagcgccgcc gcgccgggga tcggcggcgc agtagcgcgg cgtgcaatcc gtgacgaggg
     5941 ccagggcctt ttgatggccg gggatgcgca cgaccgcggc atcgccgccg ggccgctgca
     6001 cggtgtggcc catcacgaga tggtcgtact gctcccagat ccagcgcttc gaggaaaggt
     6061 cgggcgtgcc cagcagcatc ttcaagagct gaagaacgtc catctcgacc cggagctctg
     6121 tgggatgaac atcgcgctgc ggcggcgtct cgatccaggg acggtcgtag agcggtgctt
     6181 ccgtgaccag cgggcccact ggcatattcg ccgccacgac gccgtccctg cgcagcacga
     6241 gccgccccgt ctccgtcacg cggccgatga tcgcgaagtc caactcccat ttctcgaaga
     6301 tgcgccgcgc ttcgtcctcg ctgccgggct tgaggatcat gagcatgcgc tcctggctct
     6361 cggagagcat gatctcgtag gcggtcatgc cgtcttctcg caccggcacg cgatcgaggt
     6421 cgagctcgat gccgagtccg cccttgccgg ccatctcaac cgaggacgag gtgaggccgg
     6481 cggcgcccat gtcctggatc gcgacgatag cgtcgcccgc catcagctcg agacaagcct
     6541 cgatcagcag cttctccgtg aagggatcgc cgacctggac ggtcgggcgc tttgcctcgg
     6601 cgtcctcgtt gaattcggcg gatgccatgg tcgcgccgtg gatgccgtcg cgcccggtct
     6661 tggcgccgac atagatcacc ggattgcctg ggccggcggc ggccgaatag aagatgcgcc
     6721 tggcatcggc gacgccgacc gtcatggcgt tgacgaggat gttgccattg taggaggggt
     6781 ggaaattgca ttcgccgccg acggtcggca cgccgacgca attgccgtaa ccgccgatcc
     6841 cggcgaccac gcccgcgagc agatggcgcg tcttcggatg atcgatgctg ccgaagcgca
     6901 gcgcgttcag attggcgatg ggccgcgcgc ccatggtgaa gacgtcgcgc aggatgccgc
     6961 cgacgccggt ggccgcaccc tgatagggct cgatgaaaga cggatgattg tggctctcca
     7021 tcttgaagat ggcggcctgg ccgtcgccga tgtcgatgac gccggcattc tcgcccgggc
     7081 cgcagatgac gcgcggcccc gttgtcggca ggcgcttcag ccatttcctc gaggatttgt
     7141 aggagcagtg ctcgctccac atcaccgaga agatgccgag ctcggtgagg ttgggctctc
     7201 gctccatgat cttgaccgcc cgggcgaact cctcgggcgt caatccatgc tgcgcgacga
     7261 tatcggggtc gattttcggt ggggcgccgg gcccggtcaa gccagcgcct ccaccagccc
     7321 gtcgaagagc gcgcagccat ccgtgccgcc gaggagcgga tcggtcgcgt cctcgggatg
     7381 cggcatcagc ccgaggaccg tcttgctctc gttgaagatg ccggcgatgt tgcgcgccga
     7441 gccattggcg ttgccggcct cgctcacctc gccttcgggc gtgcaatagc ggaacgcgac
     7501 gaggcccttg tcttcgagcc gatcgagggt ctcggcatcg gcaaaatagt tgccgtcgtg
     7561 atgcgccacg ggcgcgcgga tcacttgtcc ggtctcatag cggcaggtga agatgctctg
     7621 gctggtctcg acccggagat cgacgttctt gcagtggaat ttgagcgtct tgttggcgag
     7681 gagcaccccc ggcagcattc ccgcctcggt cagaatctgg aagccgttgc agatgccgag
     7741 caccggcgtg ccgcgcgcgg ccgcggcctt gacgtcgcgc atgatcggcg aatgcgccgc
     7801 catggcgccg cagcgcagat agtcgccgta ggcgaagccg ccgggaatga cgatgagatc
     7861 cgccttgggc aaggcgctgt cgcgatgcca gaccttgaga ggcgcccgtc cggcggcgcg
     7921 agcgagcgcg tcatggacgt cctgctcgcg gttggacccg ggaaagatga tgatggcggc
     7981 ttgcatcggc agggctgaga taacgcgccc gggcgtcgcg atcaagccac gtcgagctgc
     8041 ggaacggccg tgcgtccttc gacaagctca ggatgaggaa gaccggatat ggcaccgacc
     8101 ggtgcccgac tcgctctacc tcatcctgag cttgtcgaag gacgcacggc cgcacagcat
     8161 cgaatcgttg gctctctgac taactctact gcttcttgcg gcgtgcgggt ttgcgccggc
     8221 cgtttgctgc tttcttgcgg ccgcgccggt tgcgcgactg gcccgactcg gacatggcga
     8281 tggcgatcgc ctggcgccgg ctcttcacct tgcgccgtcc gccgctcttg agctcgcctt
     8341 ccttgtattc acgcatcact ctcttgacct tgcgtcgctg ttttggactc gccatcgagc
     8401 aactccctgt cagtgcttct atggcgagaa acgacccatc gcgcgccgcg ttcccgcgca
     8461 acgcgccatc cgactgtgtc cttcatcgaa aagcgagagg gctggcgcaa acgaccgcgc
     8521 cgcaccaatt gccatgggcg ctgaacgcag tgtcagtcgc ggagctcgat ctggtagctc
     8581 tcgatgacgg tgttggcgag gagcttctgg cacatctgct cgacagcggc gcgggcgcgc
     8641 gtcgcgtcgg cctcggcaag atcgagatcg atcactttgc cttggcgcac ctcgtttacg
     8701 ccggcaaagc cgagcgatgc caaggcatgc ccgatcgcct tgccctgggg atcgagcacg
     8761 ccgttcttca gcatgacatg gacgcgcgct ttcattgcac cgtctgcggg cccttgatgt
     8821 ccgtcggtcc g
//
LOCUS       DASYNS010000277         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4902629, whole genome shotgun sequence.
ACCESSION   DASYNS010000277 DASYNS010000000
VERSION     DASYNS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4902629"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VGP50_08660"
     CDS             complement(<1..204)
                     /locus_tag="VGP50_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV8027489.1"
                     /translation="MLEGFARREVRTSGATIVTVVGGSGPPLLLLHGNPFTHLSWHAV
                     APVLARDFTVVCTDLRGYGDSSKP"
     gene            complement(215..1927)
                     /locus_tag="VGP50_08665"
     CDS             complement(215..1927)
                     /locus_tag="VGP50_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV8027490.1"
                     /translation="MAPAAMNGAVLPRARVDLSGLVFASLALLLCALVLLPLAWLVWF
                     GITDKSGAATLGNFTTLAADPSFTRPFVLTLGVALAVSLLSCLVATPLAWLVARTDMP
                     GRRTVRLLVMASFVTPPFLGAIAWEILAAPNSGALNQWYRALFDLEADAHVFDIYTVE
                     GLVFVMTCYAFPYVFVLAANALERIPADLEDAAAILGSGRWQSFRRISLPLVMPSLLA
                     GALVAFLQAMTQFGAPAILALPAGFHVITTRIWSLFQYPPEPHLAAAASLPLLLLTVL
                     LLRGQHSLLRGRGYVVIAGKGGAMRRISLGRWRWPAFAAALAVLALPVLLPYAALFKT
                     ALVPTASDALTWSTLTLRNFRFVFEEFSATRLALANTLILGLAAATAGSALALVSAFL
                     AARRVVPGAALLGALASAPAAIPGIVLGVGLFLAYTRPPFVLYGSLAILLLAFVTIEL
                     PAAYQQLRSALAGLHPELEEASRILGASRGRALWQITTPLLGSSVIATWCFIFIGAIR
                     ELSATIMLTTADTKLVSVIIYDLNESGDLGAISVLGITLLAITFIVVAIANRLPRRRG
                     DAMT"
     gene            complement(1974..3038)
                     /locus_tag="VGP50_08670"
     CDS             complement(1974..3038)
                     /locus_tag="VGP50_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027491.1"
                     /translation="MAAVEVEHLTKRFGARPAVDDLTLKVEHGLFACLLGPSGCGKTT
                     ALRLIAGFLAPDDGAIRVGERLLSSRNGVVPPEKRNMSMIFQSYALWPHMTVHENVAY
                     GLRLRRLARTEIARRVDDILAVTRLRAEADRYPADISGGQQQRVALARALVVEPEILL
                     LDEPLSNLDASLREEMRFEIRRLHDAYRYTTLYVTHDQAEAMTTADLIVVMNQGRIEQ
                     LGSPEEVYERPRTEFVARFLGGTNILRGRRIAEGLVDCGSVVLRCGTGELGHREETIV
                     AIRLHHVDLSIDGGRALPVNEANGTVARRIYLGAQRDYLVALADGQQLRVTTPPSVDI
                     PVGAAVKLRLPPEHCRALPE"
BASE COUNT          569 a          985 c         1145 g          409 t
ORIGIN      
        1 cggcttcgag ctgtcgccgt agccgcggag gtcggtgcaa accacggtga agtcgcgcgc
       61 cagaacgggg gcgacggcat gccaggacaa atgcgtgaag ggattgccgt ggagcagcag
      121 cagcggcggt ccgctgccgc cgaccacggt gacgatggtg gcgccgctgg tgcggacctc
      181 gcggcgggca aagccttcga gcatggcgct cccctcaagt catggcgtcg ccgcgtcggc
      241 gcggcaggcg gttggcgatg gcgacgacga tgaaggtgat ggcgaggagc gtgatgccga
      301 gcacggagat cgcgcccaga tcgccgctct cgttcaggtc atagatgatc accgagacga
      361 gcttcgtgtc ggcggtggtc agcatgatcg ttgccgagag ctcgcggatc gcgccgatga
      421 agatgaagca ccaggtggcg atgacgctgc tgccgagcag cggcgtggtg atctgccaga
      481 gcgcccgccc gcggcttgcg ccgaggatgc ggctcgcctc ctcgagctcg ggatggaggc
      541 cggcgagtgc cgagcgaagc tgctgatagg cggcgggcag ctcgatggtg acgaaagcaa
      601 ggagcaagat cgcgaggctg ccatagagca cgaagggcgg ccgggtataa gccaggaaca
      661 ggccgacgcc gaggacgatc ccggggatcg ccgcgggcgc gctggcgaga gcgccgagga
      721 gcgccgcccc gggaacgacg cggcgcgccg cgaggaacgc gctgacgaga gcaagcgcgc
      781 tgccggccgt cgccgcggcc agcccgagga tcagcgtgtt ggccagcgcc agccgcgtcg
      841 ccgagaattc ctcgaagacg aagcggaagt tgcgcagcgt cagcgtgctc caggtcagcg
      901 cgtcggatgc cgtcggcacc agcgctgtct tgaagagcgc cgcgtaaggc agcagcaccg
      961 gcagcgccag cacggcgagc gcggcggcga acgcgggcca gcgccagcgg ccgaggctga
     1021 tgcgccgcat cgcgccaccc ttgccggcga tgacgacata gccgcgaccc cgtagcagcg
     1081 agtgctggcc gcgcagcaag agcaccgtca ggagaagcag cggcagcgag gcggcggctg
     1141 ccagatgcgg ttcgggcgga tactggaaaa ggctccagat tcgcgtggtg atgacgtgga
     1201 acccggccgg cagcgccagg atcgccggcg caccgaactg cgtcatcgcc tgaaggaagg
     1261 cgacgagcgc gcccgccagc agcgatggca tgacaagcgg caggctgatg cggcggaagc
     1321 tctgccagcg gccgctgccg aggatggccg ccgcatcctc gagatccgcc ggtatccgct
     1381 cgagcgcatt cgcggcgagc acgaagacat acgggaaggc atagcaggtc atgacgaaga
     1441 cgaggccctc gaccgtatag atgtcgaaga catgcgcgtc ggcctcgaga tcgaagagcg
     1501 cgcgatacca ttggttcagc gcgccactgt tgggtgccgc gagaatctcc cacgcgatgg
     1561 cgccgaggaa gggcggcgtc acgaaggacg ccatgacgag aagccgtacc gtccgccgtc
     1621 cgggcatgtc ggtgcgcgcg accagccagg cgagcggggt cgccaccagg caagagagca
     1681 gcgacacggc gagcgctaca cccagcgtga ggacgaaagg ccgcgtgaag ctcgggtcgg
     1741 cggcaagcgt cgtgaaattc cccagcgttg ccgcgccgct cttgtcggtg attccgaacc
     1801 agacaagcca agccaacggc agcaaaacga gcgcgcagag aagaagtgcc agcgatgcaa
     1861 agacgaggcc cgagagatcg actcgagcgc gcggcagaac ggcgccgttc atcgcggccg
     1921 gcgccaatga cgccctctcc tcttgcggga gggtgagggg tgacgtgccg ctctcattcc
     1981 ggcaaggcgc ggcaatgctc gggcggcagg cggagcttga ccgcggcgcc gacgggaatg
     2041 tcgacgctgg gcggcgtcgt gacgcggagc tgctggccgt ccgccagcgc tacgagatag
     2101 tcgcgctgcg cgccgagata gatgcggcgc gcgaccgtgc cgttcgcctc gttgaccggc
     2161 agagcgcggc cgccatcgat ggagagatcg acgtgatgga gccggatggc gacgatcgtt
     2221 tcctcgcggt ggccgagttc gccggtgccg cagcgcagca caacgctgcc gcaatcgacg
     2281 agcccctcgg cgatacggcg cccgcgcagg atgttggtgc cgcccaggaa gcgcgcgacg
     2341 aattccgtgc gcggccgctc atagacctct tcgggcgaac ccaactgctc gatgcggccc
     2401 tggttcatca ccacgatgag gtcggccgtc gtcatcgcct cggcctggtc gtgggtgaca
     2461 tagagcgtcg tatagcgata ggcgtcgtga agtcggcgga tctcgaagcg catctcctcg
     2521 cgcagactgg catcgagatt ggagagcggc tcgtcgagga gcaggatctc gggctcgacc
     2581 acgagagcgc gggcgagcgc cacgcgctgc tgctgcccgc cggagatgtc ggcgggatag
     2641 cgatcggcct ctgctctgag ccgggtcacc gccaggatgt cgtcgacccg tcgcgcgatc
     2701 tcggtcctgg cgaggcgacg cagccgcagg ccgtacgcga cgttctcgtg gaccgtcata
     2761 tgcggccaga gcgcgtagct ctggaagatc atcgacatgt tgcgcttctc gggcggcacc
     2821 acgccgtttc gcgatgagag caggcgctcg ccgacgcgga tcgccccgtc atcgggcgcg
     2881 agaaagcccg cgatgagccg gagcgccgtc gtcttgccgc agcccgacgg cccaagcagg
     2941 caggcgaaga ggccatgctc gaccttgagc gtgaggtcgt cgaccgcggg ccgcgcgccg
     3001 aagcgcttcg tcagatgctc gacctcgacc gccgccatcc tccctactca aaagcgatag
     3061 cggacgccga gcgagacggc gtggctcgtg aattccgagt tgaagctg
//
LOCUS       DASYNS010000278         4493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_65967, whole genome shotgun sequence.
ACCESSION   DASYNS010000278 DASYNS010000000
VERSION     DASYNS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65967"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..317)
                     /locus_tag="VGP50_08675"
     CDS             complement(105..317)
                     /locus_tag="VGP50_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027492.1"
                     /translation="MAPPAVDHSLAFHRLQEARQELAQARSGFPAMLPRDRARLVARC
                     EANVTRMKRWLARTSGVDVPSLPPET"
     gene            complement(370..570)
                     /locus_tag="VGP50_08680"
     CDS             complement(370..570)
                     /locus_tag="VGP50_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027493.1"
                     /translation="MQYRCYFLDQSDAISCHDDIECAGDGEAAQLALGMLSERSRDHA
                     VEVWERARRILYQVRAMSRREQ"
     gene            complement(645..878)
                     /locus_tag="VGP50_08685"
     CDS             complement(645..878)
                     /locus_tag="VGP50_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027494.1"
                     /translation="MVEKRTGAEHHVLAAEHHEHAARHHRQASEHYGEKDFAHAAHES
                     LIAHDHTRRAIHHSNEAGKYHVERHDNAKLCEL"
     gene            complement(897..1076)
                     /locus_tag="VGP50_08690"
     CDS             complement(897..1076)
                     /locus_tag="VGP50_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027495.1"
                     /translation="MITAVEVIECADDGDAQQGALRLLRQRSPYAVEVWDDSRKVFHA
                     RGRDVEADMRAVSAG"
     gene            1546..2472
                     /locus_tag="VGP50_08695"
     CDS             1546..2472
                     /locus_tag="VGP50_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEV8027496.1"
                     /translation="MRLLTVRHVTVYRYAQPVALGEHRMMFRPRESHDLRLIASRLEI
                     LPRPTQLRLVHDVFDNSVAVATFSKRTAELRFDSTVTLEHIETELPDYALERAAATYP
                     FAYAQDDLPDLAQARRRHSPAADVDHWAASFVPRSGSTDTVALLRAMTVGIREQLAYT
                     RRTERGVQTPSETLRRGSGSCRDFAWLMIEAVRSLGFAARFVSGYIFVPDFTPSDVLG
                     GGATHAWLQIYLAGAGWIDFDPTNRIIGNRNLIRVAVAWNPAQALPLWGTFIGSAASC
                     LGMEVAVTVIEETRPAQARPSARRRPRTASGI"
     gene            2534..2791
                     /locus_tag="VGP50_08700"
     CDS             2534..2791
                     /locus_tag="VGP50_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEV8027497.1"
                     /translation="MITAHVPGAMADKIDEIAARLERPRGWIVKQALAAWIATEEEHR
                     RLPHAALSDVDAGRVIDHQAVEAWAMNLGTDHPLSPPRPPE"
     gene            complement(2808..3761)
                     /locus_tag="VGP50_08705"
     CDS             complement(2808..3761)
                     /locus_tag="VGP50_08705"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV8027498.1"
                     /translation="MDSQALLTFVTIHRAGGLSAAAERLSRTQPAISRRLALLEGEVG
                     APLFERIAGGVVLSEAGRALLPHAERVLSAVRDAESAMHALRAEGGGPVSLAAVGTLA
                     GANLTAVLRIFGAEHPNADLSIRTATSAEVSDIVRRGDATIGLRYLLDPASDLVCDLI
                     GTETLRVVCRADYKLAGKAIKSLATLRDETWLAFPSAYVRRETYADNIFAQFQAAGVG
                     DIKWRRVDSLSAQKRLIEAGFGLALLPDSAVLEEVRAGTLATVDVLDLRAGNPVYAVT
                     RKGGYLSPASERLLALLRQLGLAQPRSGVSSSARSRRSKRR"
     gene            4025..>4493
                     /locus_tag="VGP50_08710"
     CDS             4025..>4493
                     /locus_tag="VGP50_08710"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEV8027499.1"
                     /translation="MTSLPRDAHARAFKALHEATPGFIMPNAWDAGSAIVLADSGFKA
                     IATTSGGIAFSLGKPDYTPSAAGFAVSRAAMFARIREIVAAVDLPVNGDLEAGYGDSP
                     EAVAETIRMAIAAGLAGGNIEDRRPDAKALYDEGLAVERIAAAREAIAAAGSAF"
BASE COUNT          732 a         1528 c         1476 g          757 t
ORIGIN      
        1 cgtcgctcgt cgcttacgat gcgcatggca tcgctgcgct cctcgctcct agcccagcgg
       61 caaatgccga tgtcgcagac cccttccatt actcagacag gctcctaggt ctccggaggg
      121 agggatggca cgtcgacccc cgaggtgcgc gccagccacc gcttcattcg cgtgacgttt
      181 gcctcgcaac gagccacgag gcgggcgcga tcgcgcggca gcatggccgg aaagccggag
      241 cgtgcctgtg cgagctcctg gcgcgcctcc tggaggcggt gaaacgccag ggaatgatcc
      301 actgcgggcg gtgccatgat cccctctccg gttgggcgcg cggtggccat tggtcgcgtg
      361 gcgcggctgc tactgttcac gccgggacat tgcccgaacc tggtagagga tccggcgggc
      421 ccgctcccat acctcgacgg cgtggtcgcg tgatcgctcg ctcagcatcc cgagcgcgag
      481 ttgcgccgcc tcgccgtcgc cggcgcattc gatatcgtca tggcacgaaa tggcgtcgga
      541 ttggtcgagg aaatagcagc ggtattgcac gatgcccctc catggtcccg tctcagcggc
      601 agcccgcgtg gccgccgcgc ccaggtggct cgcaaggtgc tccatcaaag ctcgcaaagc
      661 ttggcgttgt cgtgccgttc gacatggtat ttgcctgcct cgttgctgtg atggatggct
      721 cggcgcgtat ggtcatgcgc gatgagcgac tcatgcgccg catgggcgaa gtccttctcc
      781 ccgtagtgct ccgaggcttg ccggtgatga cgcgccgcgt gctcgtgatg ctctgcggcg
      841 agcacgtgat gttctgcgcc ggtgcgcttc tcgaccatga ttcctccgct gtcgcctcag
      901 ccggctgaaa cggcgcgcat gtcggcttcg acgtcgcgac cgcgggcatg aaagaccttt
      961 ctggagtcgt cccagacctc gacggcgtag ggagaccgct ggcgcagcag ccgcagcgct
     1021 ccttgctgcg cgtcgccgtc gtcagcgcac tcgatgacct cgaccgccgt gatcattttg
     1081 tgcgtattca tgagaaagca ctgataagcg cgcattcggc gcctcctccg tctgataagc
     1141 cctcgcgcgc aggagaggac gccgcaatgt ccgccgcagg tcgccgtatc tggcgggtcg
     1201 cgcagctcct cgggaccgag ctgccgcatt cattacatgt gggcgccacg cggcgctttt
     1261 acatcacggt aaaaaacaat atttccgctc gtcgcgcaga tttcggcaat gggcgcaaaa
     1321 tctcgaccac agccgcgcgg cggcgttcgc gcgtttttcg tcgcgttcga ttgccgccgc
     1381 attcgcttat ccatgggcgc cggcgaattt ctgggcggct gcttggatcg ctcctatatg
     1441 accattcgct cgcgcagcgt cccgatggta ggttgtgagt gcggcgcggc tcgcgtgacc
     1501 ggaagaccgg gtgcagaagc ggccagcgca gcggatcgga tcggtatgag actcctcacc
     1561 gtgcggcacg tcaccgtcta ccgctatgcc cagccggtcg cgctgggcga gcatcgcatg
     1621 atgttccggc cgcgcgagag ccacgacctc cgcctgatcg cgtcgcggct cgagatcctg
     1681 ccgcggccga cccagctgcg cttggtccat gacgtcttcg acaactcggt cgccgtcgcg
     1741 accttctcca agcgaacggc cgagctccgt ttcgacagca ccgtcacgct tgagcatatc
     1801 gaaaccgagc tgcccgatta cgccctcgag cgcgcagcgg cgacctatcc cttcgcctat
     1861 gcgcaggacg acctgcccga cctcgcccag gcgcggcggc ggcactcccc tgccgccgac
     1921 gtcgatcatt gggcggcgag cttcgtgccg cgatccggct cgaccgacac ggtcgcgctg
     1981 ctgcgcgcga tgacggtcgg catccgggag caactcgctt atacacgccg caccgagcgc
     2041 ggcgtgcaga cgccgagcga gaccttgcgg cgcggcagcg gctcctgtcg cgacttcgcc
     2101 tggctcatga tcgaagccgt gcgctccctc ggctttgccg cgcgcttcgt cagcggctac
     2161 atcttcgtgc ccgacttcac gccgtcggac gtgctgggcg gcggggcgac ccatgcgtgg
     2221 ttgcagatct acctggcggg cgccggatgg atcgatttcg atccgaccaa ccgcatcatc
     2281 ggcaaccgca acctcattcg cgtcgcggtg gcctggaacc cggcgcaggc cctgccgctc
     2341 tggggcacct tcatcggctc cgccgcgtcc tgcctcggca tggaggttgc cgtgaccgtc
     2401 atcgaggaga cgcgccccgc gcaagcgcgc ccgagcgcaa gaagacggcc gaggacagca
     2461 tcgggcatat agacggcgcc gagcagctat cgcacaagga tgtcgagaca tggccgccaa
     2521 acgcgacacc cggatgataa cggcacatgt accgggcgcc atggccgaca agatcgacga
     2581 gatcgccgca cgactcgagc ggccgcgcgg ctggatcgtg aagcaggccc tggcagcgtg
     2641 gatcgccacc gaagaggagc accgccggct gccccacgcg gcgctctccg atgtcgacgc
     2701 cggacgggtg atcgaccatc aggcggtcga ggcctgggcg atgaacctcg ggaccgatca
     2761 tccgctttcg ccgccccgac cgcccgaatg aggctcccat ggaccagtca tcggcgcttt
     2821 gatcgccgac ttcgcgcgga tgaggaaacg ccggaccgcg gctgagcgag tccgagctgt
     2881 ctcaggaggg cgagcaggcg ctcgctggcc gggctgagat acccgccttt ccgggtgacg
     2941 gcgtataccg ggttgccggc gcgcagatcg aggacatcga ccgttgcgag cgttccggcc
     3001 cgcacttcct cgagaacggc gctatcgggc aggagcgcga gaccgaaacc ggcctcgatg
     3061 aggcgctttt gcgcgcttag gctgtcaacc cgccgccatt tgatatcacc cacgcccgct
     3121 gcctgaaatt gggcaaagat attgtcggca taggtttcgc gccgcacata ggcgctcgga
     3181 aatgccagcc aggtttcgtc gcgcagcgtc gccagggatt tgattgcctt gcccgcgagc
     3241 ttgtaatcgg cgcggcagac gacgcgcagc gtctccgttc cgatgagatc gcatacgaga
     3301 tcggacgcgg ggtcgaggag atagcgcagc ccgatcgttg cgtcgccgcg ccggacgatg
     3361 tcgctgacct ccgcgctcgt cgcggtgcga atcgaaagat cggcattcgg gtgctcggcg
     3421 ccgaagatcc gcagcaccgc ggtgagattg gcgccggcga gcgtgccgac ggcggcgagc
     3481 gagaccgggc cgccgccctc cgcgcgcagc gcatgcatcg cgctctctgc gtcccgaacg
     3541 gccgacagca cccgttcggc atggggcagc agcgcgcggc cggcttcgct cagcaccacg
     3601 ccgcccgcaa tgcgctcgaa cagcggggcg ccgacttcgc cctccagcaa tgccagtcgc
     3661 cggctgatcg ccggttgagt tcgtgaaagt ctttcggctg ccgcggaaag gccgccggcg
     3721 cgatggatgg taacaaaggt gagcagcgcc tgagaatcca tgaggaccca taccgcaagc
     3781 ggtgcgggag gcggccggtg ccaatccggc gttcgacctt tccgcgatgc aaaatcctaa
     3841 tggtagtcag aagatacatc aattggacgc ataggtccag gcgtgacagg gtcttgcctc
     3901 aagccaggag gaacgccgac atggcagagt gtctcgcagg cgggccggtg catgccgagc
     3961 tcgagagcaa cgtcgtctat cgcccatccg atcatctccg cataccggca ttcgaggcct
     4021 gacgatgacc tcccttccgc gcgacgcgca cgcccgcgcc ttcaaggcgc tgcacgaagc
     4081 caccccgggc ttcatcatgc caaacgcctg ggacgccggc agcgccattg tccttgccga
     4141 ttccgggttc aaggcgatcg cgacgacgag cggcggcatc gccttctcgc tcggcaaacc
     4201 cgactacacg ccgagcgccg cgggcttcgc cgtatcgcgc gcggcaatgt tcgcccgcat
     4261 ccgcgagatc gtggccgccg tcgatctgcc ggtgaacggc gacctcgagg ccggctatgg
     4321 cgattcgcca gaggcggtgg cggagaccat ccgcatggcc atcgcggccg gcctcgccgg
     4381 gggcaatatc gaggataggc ggccggacgc gaaggcgctt tatgacgagg ggctggccgt
     4441 cgagcgcatc gccgcggcac gcgaagcgat cgccgccgcc ggcagcgcct tcg
//
LOCUS       DASYNS010000279         5731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3343091, whole genome shotgun sequence.
ACCESSION   DASYNS010000279 DASYNS010000000
VERSION     DASYNS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3343091"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /gene="rpsP"
                     /locus_tag="VGP50_08715"
     CDS             complement(<1..248)
                     /gene="rpsP"
                     /locus_tag="VGP50_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV8027500.1"
                     /translation="MSLKIRLTRGGTKKRPFYSIVVADSRSPRDGRFIEKLGTYNPML
                     DKGHADRVVLKAERIQHWIKVGALPSERVQRFLGDAGLG"
     gene            complement(274..663)
                     /locus_tag="VGP50_08720"
     CDS             complement(274..663)
                     /locus_tag="VGP50_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV8027501.1"
                     /translation="MIDHLSLGVADLGRSRAFYDAALAPLGYRRLFDVDDASGYGAAL
                     PAPLREQALPFWVGQDTGRIGLSGHVCFTAARRAAVDAFYEAALAAGGRDNGKPGLRP
                     IYHPNYYAAFVVDPDGHRIEAVCHQPE"
     gene            complement(660..2033)
                     /gene="ffh"
                     /locus_tag="VGP50_08725"
     CDS             complement(660..2033)
                     /gene="ffh"
                     /locus_tag="VGP50_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV8027502.1"
                     /translation="MFESLSNRLSDVFDRLRRHGALSEDDVTAALREIRIALLEGDVA
                     LPVVKDFVNAVREKALGQEVLRSVSPAQMVVKIVHDHLVETLGGDAVEGLNLNAVPPV
                     VIMMVGLQGSGKTTTAAKLARLLKLRERKKVLLASLDVQRPAAQEQLAVLARQVEVAS
                     LPVVVGEKPVAIARRALDVGRREGYDVVILDTAGRLHIDEPLMQEVAAVRDATQPTES
                     LLVADAMTGQDAVNVAKSFAERVGLTGIVLTRVDGDARGGAALSMRAVTGRPIKFMGT
                     GEKLDALEPFHARRVAGRILDLGDIVSLVEKAQETIDQDEAEALAKKLQKGGFDLDDL
                     AAQLRQLRKMGGMGGVMGMLPGIGKIKKQLEEARIDDGMIKRQEAIISSMTRAERKNP
                     KLLNASRRIRIASGSGTSVPEINRLLKQYQDMSSMMRKMNKLGKKGLMRHGLGALLPG
                     GGGGFGR"
     gene            2258..3406
                     /locus_tag="VGP50_08730"
     CDS             2258..3406
                     /locus_tag="VGP50_08730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEV8027503.1"
                     /translation="MNTDKAVNIADLKRMAKRHMPRVVFDFVEGGLEDEHGLQHNEDA
                     FKRHRLLPRYLVDTGKRELATTLFGRSYALPFGIAPTGLAALCRPGADLMLAEAAAAA
                     NVPFIMSGASTASIEAAARVAPQHCWYQLYAARDPKISEDLIRRTRDAGLAALVLTVD
                     VPVNSKRERNIRNGFVRPYKLKPAVLLEALRHPSWIADYLRHGKPVFANWAPYAPPGA
                     GSDEVADFLQSQTPSVQTWRDLETYRRLWPRTLIVKGILHPDDARRAADLGVDGIMVS
                     NHGGRQLDRAPSPLEMLLPIKQAVGARLTVMFDSGIRRGSDIVIARCLGAQFIFLGRA
                     TLYGAAAGGVAGAKRAIDILRDEVDRALIQLGCPNFNELGPNWLVNES"
     gene            3523..3849
                     /locus_tag="VGP50_08735"
     CDS             3523..3849
                     /locus_tag="VGP50_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027504.1"
                     /translation="MSTNLSLFSRAVAVAGVLALSLPAFAQTNQPAAAPTSQPASPVA
                     SPASPAPAVNKGTDKQSSTKTTKDKHAAKKPLVKTAKNVDTHKAVKPAAVTKTEQQPA
                     TGGVAK"
     gene            4118..4951
                     /gene="dapF"
                     /locus_tag="VGP50_08740"
     CDS             4118..4951
                     /gene="dapF"
                     /locus_tag="VGP50_08740"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEV8027505.1"
                     /translation="MRGIPFIKMHGLGNDFVVLDGRDGKIALDDKAARAIADRHTGVG
                     CDQLIILEPPRTRGADLFMRIHNADGGEVEACGNAARCIADLVMRDKGKDSTVIETAA
                     GLLEAHAAGARRVAVDMGAPRTGWREIPLARECDTLHVTLALGPLADPVCTSIGNPHA
                     TFFVANADAIDLAALGPKLEHDPLFPERANIGIAQVVSPQRLRLRVWERGAGITPACG
                     TGACAALVAAHRRGLAERKAEVIADGGALEIEWRTDGHVLMTGPVAVSFTGRLDPSLL
                     G"
     gene            4956..>5731
                     /locus_tag="VGP50_08745"
     CDS             4956..>5731
                     /locus_tag="VGP50_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV8027506.1"
                     /translation="MGQLDVVTFGCRLNAFESEVIRRQAEAAGLGDAVIVNTCAVTAE
                     AERQARQAIRRARRERPEARIIVTGCAAQIDPARYAAMPEVDQVLGNEAKLRAESYAR
                     SAPPLLVDDILAAKETAAHLVDGFEGRARAFLQVQQGCDHRCTFCVIPLGRGNNRSVP
                     MGAIAEQLRRLVAQGYREVVLTGVDITGFGADLPGRPSLGQLVRRLLAAVPELERLRL
                     SSLDPVEIDDALWRLIAEEPRLMPHLHLSLQAGDDLVLKR"
BASE COUNT          915 a         1993 c         1918 g          905 t
ORIGIN      
        1 ccgaggccgg catcgcccag gaagcgctgc acccgctcgc tcggcaaagc gccgaccttg
       61 atccaatgct ggatgcgctc cgccttcagc acgacgcggt cggcatgccc cttgtcgagc
      121 atcgggttgt aggtcccgag cttctcgatg aagcggccgt cgcgagggct gcgggaatcg
      181 gcgacgacga tgctgtagaa ggggcgcttc ttggtaccgc cgcgggtcaa acggatcttg
      241 agggacatgc ttctcctttc acgcgatgct cggtcattcg ggctgatggc agaccgcctc
      301 gatccgatga ccgtcgggat cgacgacgaa ggcggcgtaa tagttgggat gatagatcgg
      361 gcgcaggccc ggcttgccgt tgtcgcggcc gccggcggcg agcgccgcct cgtagaaggc
      421 gtcgaccgcc gcgcgccgcg ccgccgtgaa gcagacatgg ccgctgagcc cgatgcgccc
      481 ggtgtcctgg ccgacccaga acggcaaggc ctgctcgcgc agcggcgcgg ggagtgctgc
      541 gccgtagccc gaagcatcgt cgacgtcgaa gagccggcga tagccgagcg gggccagcgc
      601 cgcgtcgtag aaggcgcggc tccggccgag atcggcgacg cccagggaaa gatggtcgat
      661 catcgcccga acccgccccc gccgcccggg agcagcgcgc ccaagccgtg gcgcatcagc
      721 cctttcttcc cgagcttgtt catcttgcgc atcatgctcg acatgtcctg atactgcttc
      781 agcaggcgat tgatctcggg caccgacgtg ccggagccgg aggcgatgcg aatccggcgc
      841 gacgcgttga gcagcttcgg attcttgcgc tcggcccgcg tcatcgagga gatgatcgcc
      901 tcctggcgct tgatcatgcc gtcgtcgatc cgcgcctcct cgagctgctt tttgatcttg
      961 ccgatgcccg gcagcatgcc catgacgccg cccatgccgc ccatcttgcg cagctggcgg
     1021 agctgcgccg cgaggtcgtc gagatcgaag ccgcctttct gcagcttctt ggcgagcgcc
     1081 tcggcttcgt cctgatcgat cgtctcctgc gccttctcga ccaggctcac aatgtcgccg
     1141 agatcgagga tgcggccggc gacgcgccgc gcatggaaag gctcgagcgc gtcgagcttc
     1201 tcgccggtgc ccatgaactt gatcggacgc ccggtgaccg cgcgcatgga gagcgcagcg
     1261 ccgccccgcg catcgccatc gacgcgcgtc agcacgatgc cggtgaggcc gacgcgctcg
     1321 gcgaagctct tggcgacatt gaccgcgtcc tgcccggtca tggcgtcggc aacgagcagg
     1381 ctctccgtcg gctgcgtggc gtcgcgcacg gcggccactt cctgcatcag gggctcgtcg
     1441 atatggagcc ggccggcggt atcgaggatg acgacgtcat aaccttcgcg ccgcccgaca
     1501 tcgagcgcgc gccgcgcgat cgccaccggc ttctcgccga caaccacggg gagcgacgcg
     1561 acctcgacct ggcgcgccag cacggcgagc tgctcttgcg ccgccggccg ctgcacgtcg
     1621 agcgaggcga gcagcacttt cttgcgctcg cgcagcttca aaagacgggc gagcttcgcc
     1681 gcggtggtgg tcttacccga gccctgcagc ccgaccatca tgatcacgac cggcggcacg
     1741 gcgttgaggt tgaggccttc gaccgcgtcg ccgcccagcg tctcgacgag atggtcgtgg
     1801 acgatcttca ccaccatctg cgccgggctc accgagcgca gcacttcctg gcccagcgcc
     1861 ttctcacgca cggcgttgac gaagtccttg accaccggca atgccacatc gccctcgagg
     1921 agggcgatgc ggatctcgcg cagcgcggcg gtgacgtcgt cctcggagag cgcgccatgg
     1981 cggcgcagcc ggtcgaagac atcggagaga cggttgctca ggctctcgaa catggccgac
     2041 ctcgacggca aaaccgagaa ggtcagtgcg cgacactcgc ggactgaccg gccccatcgg
     2101 ggcgaattcg ttaaggctgc ttccggggaa gcgacttgaa actagggaac gccgcggttt
     2161 ctgtcaaata tgcagcgggc aacgtagcca ctcccgcgat gcgggcgagg ggtgcggaca
     2221 ctggcgcagc cagggcagtt tggctacatg agcgggcatg aataccgaca aagcggtcaa
     2281 catcgcggac ctgaagcgca tggcgaagcg gcatatgccg cgcgttgtct tcgattttgt
     2341 cgagggcgga ctcgaggacg agcatggcct tcagcacaac gaggatgcgt tcaagcgcca
     2401 ccggctgttg ccgcgctacc tcgtcgatac cggcaagcgc gagctggcga cgacgctgtt
     2461 cggccgctcc tacgccctgc ccttcggcat cgcgccgacc gggctggccg cgctgtgccg
     2521 gccgggcgcg gatctcatgc tggccgaggc cgccgcggcg gcgaatgttc ccttcatcat
     2581 gtccggcgcg agcaccgctt cgatcgaagc cgcggcgcgg gtggcgccgc agcactgctg
     2641 gtaccagctc tatgccgcgc gcgatcccaa gatctcggag gatctcatcc gccgcacgcg
     2701 ggacgccgga ctcgccgcgc tggtgctgac ggtagacgtg cccgtgaact cgaagcgcga
     2761 gcgcaacatc cgcaacggct tcgtgcggcc ctacaagctc aagccggcgg tgctgctgga
     2821 agcgttgcgg catccgagct ggatcgccga ctatctgcgc catggcaagc cggtcttcgc
     2881 gaattgggcg ccctatgcgc cgcccggtgc cggctccgac gaggtcgcgg atttccttca
     2941 atcgcagacg ccttcggtgc agacctggcg cgacctcgag acctatcggc ggctctggcc
     3001 gcgcacgctc atcgtcaaag gcatcctgca tcccgacgat gcgcgccgcg ccgccgatct
     3061 cggtgtcgac ggcatcatgg tgtcgaacca tggcggccgc cagctcgacc gcgcgccgtc
     3121 gccgctcgag atgctgctgc cgatcaagca ggcggtgggc gcccggctca cggtgatgtt
     3181 cgacagcggc atacggcgcg gctccgacat cgtcatcgcg cgctgcctcg gcgcgcaatt
     3241 catcttcctc ggccgcgcca cgctctacgg cgcagcggcg ggcggcgtcg ccggcgccaa
     3301 gcgcgcgatc gacattttgc gcgacgaggt cgaccgcgcg ctcatccagc tcggatgtcc
     3361 gaatttcaac gagctcgggc cgaactggtt ggtgaacgaa agttaaccca gccacaattc
     3421 cgccgcagaa gatcacaggt tttccacgga ctgtccgcgt gcaagggtcc gttgtcctgc
     3481 tgtctcgctc cggagacgca caaacacacg ggacctccca tcatgagcac caacctttct
     3541 ctcttctcgc gcgctgtcgc cgtcgccggc gttctcgcgc tgagcctgcc ggcctttgcg
     3601 cagaccaacc aacctgccgc ggcgccgacg agccagcccg cttctcccgt cgccagcccg
     3661 gcttcgcccg cgcccgccgt caataagggc accgacaagc agagctcgac caagaccacg
     3721 aaggacaagc acgcggccaa gaagccgctg gtgaagacgg cgaagaacgt cgacacccac
     3781 aaggcggtga agcccgctgc cgtgaccaag accgagcagc agccggcgac cggcggcgtc
     3841 gcgaagtaag cgagcgcctg caaaaaagct gcaagaaacg gcggcattcc ctccccggta
     3901 cggggaggga atgcgcgttt tcggccaacc cgcatgggtg cgggctttcg cggttgctgg
     3961 cgcaaccgct gccgcccgcc ggcgacgacg caagagcgtc gcgcagagct ttcgcggttg
     4021 ctggcgcaac cgctgacgcc cgctcgcgac gacgctccgc gtcgctcggg cgtcgcgcgg
     4081 attgtgcctg cagaggaacg gacctatata ccgcgccatg cgcggcattc ccttcatcaa
     4141 gatgcacggg ctcggcaacg atttcgtcgt gctcgacggg cgcgacggga agatcgcgct
     4201 cgacgacaag gccgcgcgcg ccatcgccga tcggcacacg ggcgtcggtt gcgatcagct
     4261 catcattctg gagccgccgc gcacgcgcgg cgccgacctt ttcatgcgca tccacaatgc
     4321 cgatggcggc gaggtcgagg cttgcggcaa tgccgcgcgc tgcatcgccg atctcgtcat
     4381 gcgcgacaag ggaaaggaca gcacggtcat cgagacggcg gccgggctgc tcgaggccca
     4441 tgcggcggga gcgcgccgcg tcgccgtcga catgggcgcg ccacgcacgg gatggcgcga
     4501 gattccgctg gcgcgggaat gcgacacgct gcatgtgacg ctggcgctgg gcccgctcgc
     4561 cgaccccgtc tgcacgagca tcggcaatcc ccacgcgacg ttcttcgtcg cgaatgccga
     4621 cgcaatcgat ctcgcggcgc tcggccccaa gctcgagcat gatccgctct ttcccgaacg
     4681 cgccaatatc ggcatcgcgc aggtggtctc gccgcagcgg ttgcgcctcc gcgtctggga
     4741 gcgcggcgcc ggcatcacgc cggcttgcgg caccggcgcc tgcgccgctc tcgtcgccgc
     4801 gcatcgccgc ggcctcgcgg agcgcaaggc cgaagtgatc gccgatggcg gcgcgttgga
     4861 gatcgagtgg cgcaccgacg gccatgtgct gatgacgggg ccggtggcgg tgagcttcac
     4921 cggcaggctc gatccatcgc tccttggttg acgcgatggg acagctcgac gtcgtcactt
     4981 ttggctgccg gctcaacgcc ttcgaatcgg aggtgatccg gcgccaggcc gaagcggcag
     5041 ggctcggcga tgcggtcatc gtcaacactt gcgccgtgac ggcggaggca gagcgtcagg
     5101 cgcgccaggc gatccgccgc gcgcgccgcg agcggccaga ggcgcgaatc atcgtcacgg
     5161 gctgcgcggc gcagatcgat ccggcgcgct acgccgccat gcccgaagtc gatcaggtgc
     5221 tcggcaacga ggcgaagctg cgcgccgaga gctatgcgcg ctccgcaccg ccgcttctcg
     5281 tcgatgacat tctcgccgca aaagagacgg cggcgcatct ggtcgacggc ttcgaggggc
     5341 gcgcccgcgc tttcctccaa gtgcagcaag gctgcgacca tcgctgcact ttctgcgtca
     5401 ttccgctggg ccgcggcaac aaccgttcgg tgccgatggg cgcgatcgcc gagcagctcc
     5461 gccggctggt ggcgcagggc tatcgcgaag tcgtgctcac cggcgtcgac atcaccggct
     5521 tcggcgcgga tctgccggga cggccgagcc tcggccagct ggtgcgccgt ctcctcgcgg
     5581 cagtgccgga gctcgagcgg ctcaggctgt cctcgctcga ccccgtggag atcgatgacg
     5641 cgctctggcg tttgatcgcc gaggagccgc gcctcatgcc ccatctccat ctctcgctcc
     5701 aggcgggcga cgacctcgtg ctgaagcgca t
//
LOCUS       DASYNS010000280         4610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_533998, whole genome shotgun sequence.
ACCESSION   DASYNS010000280 DASYNS010000000
VERSION     DASYNS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533998"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VGP50_08750"
     CDS             <1..488
                     /locus_tag="VGP50_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027507.1"
                     /translation="SPVAARVAVDALGMTPRNYGHLVMMAARYARERLDGHAVVNRQT
                     RAPVMLDWERGRKNATAPGIPPLLLLAVPAIPAMLAAGALSRRAPAATALSAACAALS
                     RLRGGSGDRRAACRRDSDRARGLAAPVVPRSCARPRSAAATRGRRRKPGCAQLGDGAS
                     R"
     gene            532..2661
                     /locus_tag="VGP50_08755"
     CDS             532..2661
                     /locus_tag="VGP50_08755"
                     /inference="COORDINATES: protein motif:HMM:NF037911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027508.1"
                     /translation="MPTTAALSVGDNATPALPPSPQDEGWFHSLLESVGLAGSTDKEP
                     PAPIPPSDNQKPVDDPPPPPPKPEFRRVPDFDEQGFPNYLPGDPYPRYEPTRPSPEPG
                     VAERVLDAAVEGAQEGWGPGRLGLSPESEQWLQDTGQFNKPGETNPIRTLNEAWMKPL
                     AAGADAILRGGSALFRGAQAAGVAAGLPRDIVAMPEAFLGSPDPLGMPRAPADVSAPE
                     MALRDSAEGKPAEAPQPNEAPPPPEGSFHDRLDDFKQFRREKGLDGEDPLSHAEAARA
                     YVMDRQGHTDDEHFVAYDANGNAITHAGTSFQKFESARPPDLEAMERDPTAKMTLHHS
                     HPNDAGLSKDDVASLPRQGMEWVAAHGVGDELSAARLTPQATTALRFGDEVGQMRAKK
                     LLRGFWNDTDDALSLPMNHAVVRGELTRDEATRISTEIVNRSLADAGIMEYVTTRTIP
                     STPFIDGMRTLANAQVRDSIRNKLPELVNHVPDSPTEPMGLRQGMARISGQDVGASSG
                     QPGRRRGGRASAGRAGPAQRQSDLGRDQSSKTSEIRRAGAHPAAVADRDGQERAGREE
                     KEVAPLPRVTGHELEMAPTSYGHLVILAARYERLRLDGHAVVNRETRVPIALTRERGL
                     KEIVAPGTPPEMLLAVPAIPAMLAGARYLGAAHDPLHRPDIAKVHGFASAVEVGGRRI
                     DVLMVVRENRQGRLFLDRVERAAARAA"
     gene            2931..4094
                     /locus_tag="VGP50_08760"
     CDS             2931..4094
                     /locus_tag="VGP50_08760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV8027509.1"
                     /translation="MKASLFYLPTVGSRRDIEAGRAGLNGDLYQQMLREVLAQVQLAD
                     ALGYDSVSFTEHHFHVEGFELSNNPVLLDLYVGLQTKRIRVGQLGIVLPASNPIRVAE
                     DIAMLDHMTGGRACAGFARGYQRRWVDVMAQQTHGIHGALPHQHDAIDAANRAAFEEC
                     FAIIKKAWTEPMLAFAGKYWKIPAGETPWALEATLKWGAGVENGLLRSVGVVPKPVQK
                     PHPPIFQPFASSERSIRWCAKEGVTAILPAMHATLEETLFDVYAEVSGRPRGQGMGLL
                     RDVIIADSDAEAVALWKDSSVFAGRAWFEPFGFRQGLIDPVTGAAPSAEESLALGYAL
                     VGTVDTVTRHLERIRRRVPLDWLFCWTFNSLVPHGVLMTSIERFWTEVMPRVA"
BASE COUNT          812 a         1513 c         1524 g          761 t
ORIGIN      
        1 ggtcgccggt cgccgcgcgc gtcgctgtcg acgcgctcgg catgacgccc aggaattacg
       61 ggcatctcgt gatgatggcg gcgcgctatg cgcgcgagcg gctcgacggg catgccgtgg
      121 tcaatcggca gacccgagcg ccggtgatgc tcgactggga gcgcggccgg aagaacgcga
      181 cggcgcccgg catcccgccg ctgctgctgc tggcggtgcc ggcgatcccg gcgatgctgg
      241 cggcgggggc gctatctcgg cgcgctcccg ccgcgaccgc cctatccgcc gcatgtgctg
      301 cgctatcacg ccttcgcggc ggcagcggag atcggcgggc ggcgtgtcga cgcgattctg
      361 atcgcgcaag aggactggca gcgccggttg ttcctcgatc gtgtgctcgg ccgcgaagcg
      421 ctgccgcaac gcggggtagg cggcgcaaac ccggatgcgc ccagctcggc gatggcgcca
      481 gccgatgatg cggcgacgcc ggatgggctg gcggcatcgc cgccggcgcc catgccgaca
      541 acggcggccc tttcggtcgg agacaatgcc actccagcgc ttccgccgtc gccgcaagac
      601 gaggggtggt tccattcgct gcttgaatcg gttgggctcg ccggttcgac cgacaaagag
      661 ccccccgcgc cgatcccgcc ctccgacaat cagaagcctg tcgacgaccc gccgccgccg
      721 ccgcccaagc ccgaatttcg ccgcgttccg gatttcgacg aacagggctt tccaaattat
      781 ctgccgggcg atccttaccc gcggtatgag ccgacgcgac catcccctga gccgggcgtc
      841 gccgagcgcg tccttgatgc agcggtcgag ggcgcacaag aaggatgggg gccgggacgt
      901 ctcggcctta gtccagagag cgagcaatgg ctgcaggaca ccggccaatt caataagcca
      961 ggtgaaacaa atcccatccg cacgctcaac gaagcgtgga tgaagccgct tgccgccggt
     1021 gccgatgcaa tcctgcgcgg cggtagcgcg cttttccggg gcgcgcaggc ggcgggcgtg
     1081 gcggccggac tgccgcgcga catcgtcgcg atgcccgaag cgtttctcgg atctcccgat
     1141 cctctcggca tgccgcgcgc acccgccgac gtttcggcgc cagagatggc gctacgagat
     1201 agtgcggagg gcaaaccggc ggaagccccg caacccaacg aggcgccgcc tccgcctgag
     1261 ggctcgttcc acgaccgcct ggacgatttc aagcaatttc gccgcgagaa ggggctcgac
     1321 ggcgaagacc ccttgagcca cgccgaagcc gcgcgcgcct atgtcatgga ccgccagggc
     1381 cataccgatg acgagcattt cgtcgcttac gacgcgaatg gaaacgccat cacccatgcc
     1441 gggacctcat tccaaaagtt cgagagtgcg cgcccgccgg acctcgaggc catggagcgc
     1501 gaccccactg cgaaaatgac gcttcatcac agtcatccga acgatgccgg cctcagcaag
     1561 gacgacgtgg cgtccctccc tcgtcaaggg atggagtggg tggccgcgca tggcgtgggt
     1621 gatgagcttt ccgccgcgcg gttgacaccg caggccacga cggctctacg gtttggcgac
     1681 gaggtcggac agatgcgcgc gaagaagctg cttcgcgggt tttggaacga tacagatgac
     1741 gctcttagcc tgccgatgaa ccatgccgtg gttcgcggcg aactcacgcg agacgaagcg
     1801 acgcggatct cgacagagat cgtgaatcgt tcgctcgcgg atgccgggat catggaatac
     1861 gtaaccacgc gcacgatccc gtccacacct ttcatcgacg ggatgcgaac gttggcaaac
     1921 gcgcaagttc gcgatagcat tcgcaacaag ctaccggagc tggtcaacca tgtacctgat
     1981 tcgcccaccg agccgatggg cctccgacaa ggaatggcaa gaatttctgg ccaagatgtt
     2041 ggagcgtcct caggacaacc cggacgtcgc agaggaggtc gagcgagcgc gggaagagct
     2101 ggacctgcgc agcggcaaag cgacctgggc cgagatcaga gctcgaagac gagcgagatt
     2161 cgacgcgctg gagcccatcc tgccgccgtc gccgaccgag acggacaaga gcgagcagga
     2221 cgggaagaaa aagaagtagc gccgctgcct cgtgtgacgg gacacgagct ggagatggcg
     2281 ccaacgagct acggccatct cgtcatcttg gcggcgcgct acgagcgcct ccggctcgat
     2341 ggtcatgccg tcgtcaatcg cgagacacgt gtgccgatcg cgctcacccg ggagcgcggg
     2401 ctcaaggaga tcgtcgcgcc ggggacgccg ccggagatgc tgctggcggt gccggcgatt
     2461 cccgccatgc tcgccggggc gcggtatctc ggcgcagcac acgatccact gcatcgcccc
     2521 gacatcgcaa aagtgcacgg cttcgcatcg gcggtcgagg tcggcggacg gcgcatcgat
     2581 gtgctgatgg tggtgcgcga gaaccggcag gggcgattgt tcctggaccg ggtggagcgc
     2641 gcggccgctc gcgcggcgta gctctcgatc gtctgacgcc gcgtcggcgg cacccctcac
     2701 cctcactgct tcgctgaagc ttcgcagtgt ttccccactg cttcgctgaa gcttcgcagt
     2761 gggtccctcc ctctctcacg aaggggagag gggaagatgc gctggccggt gctgcgcatc
     2821 atggaggcct tcgccagcgt caggcgctga gcaaccggcc ttcgctgcta tacagcgatg
     2881 gcgatctttg ccatcctttc cgcagaacaa aatgcgggag gaggcgtgac gtgaaggctt
     2941 cgctgttcta tctgccgacg gtcggcagcc gccgcgacat cgaggcgggc cgggccggac
     3001 tcaacggcga tctctatcag cagatgctgc gcgaggtgct ggcgcaggtg cagctcgccg
     3061 acgcgctcgg ctatgacagc gtgagcttca ccgagcatca cttccacgtc gaaggcttcg
     3121 agctctccaa caacccggtg ctgctcgatc tctatgtcgg cctgcagacc aaacgcatcc
     3181 gcgtcggcca gctcggcatc gtgctgccgg cgtcgaaccc gatccgcgtc gccgaagaca
     3241 tcgccatgct cgaccacatg accggcgggc gcgcctgcgc cggcttcgcg cggggctatc
     3301 agcggcgctg ggtcgacgtc atggcgcagc agacccacgg catccacggc gcgctgccac
     3361 atcagcacga cgccatcgac gcagcgaacc gcgccgcttt cgaggaatgc ttcgcgatca
     3421 tcaagaaggc gtggaccgag ccgatgctcg ccttcgcggg caagtattgg aagattcccg
     3481 cgggcgagac gccctgggcg ctcgaagcga cgctcaaatg gggcgcgggc gtcgagaacg
     3541 gcctcttgcg gtccgtcggc gtcgtgccga agccggtgca gaagccgcat ccaccgatct
     3601 tccagccttt cgccagctcc gagcgcagca tccgctggtg cgcgaaagag ggcgtgacgg
     3661 cgatcctgcc ggcgatgcat gcgactctcg aggagacgct cttcgacgtt tacgccgagg
     3721 tctcgggccg gccgcgcggc cagggcatgg ggctgctgcg cgacgtcatc atcgccgaca
     3781 gcgacgccga ggccgtcgcg ctgtggaagg attcgagcgt cttcgccggc cgcgcctggt
     3841 tcgagccctt cggcttccgc caggggctga tcgatccggt caccggcgcc gcacccagcg
     3901 ccgaggaatc gctcgccctg ggttatgcgt tggtcggcac ggtcgatacc gtcacgcgcc
     3961 atctcgagcg catccgccgc cgcgtgccgc tcgactggct cttctgctgg accttcaaca
     4021 gcctcgtgcc acacggcgtg ctgatgacgt cgatcgagcg gttctggacc gaagtgatgc
     4081 cgcgcgtcgc ttaggtcgtg tactgataat tcgggcctga cgcgacggtt cgaatgtcgg
     4141 caatgcgccc ttgccggccg ttcttggcga atgcttctgc cgctctagag cggacatcgg
     4201 aggcgcagcg ccatcggtag gatttgaact ttaagcctac gaccttcagg ttatgagttt
     4261 gactacgttt ggctcatggg actcgcggaa tcgatcctgc cgtaatggtc acctggaacg
     4321 tcgtagcgat ggcagatgtc aatcggcgag gccgaatcac acccgtcgcg gattgaacag
     4381 gttgtgagat tcttcacagc cggtcacccg acggcgtgct acttggtggg ggttgcgcgt
     4441 tgccgagttc tctcaaacac aagtcgcgcg acgaggccgc tgtcatccga tacccagctt
     4501 ttcccgctgt ccacgcgact tcgtgagacc tgttcgattc cgggaacgcc caactcgatc
     4561 taacagagac aacacatgag cataaacata ctccgcgtcc ggcggcagag
//
LOCUS       DASYNS010000281         4937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1001665, whole genome shotgun sequence.
ACCESSION   DASYNS010000281 DASYNS010000000
VERSION     DASYNS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001665"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..981)
                     /gene="lpxA"
                     /locus_tag="VGP50_08765"
     CDS             complement(178..981)
                     /gene="lpxA"
                     /locus_tag="VGP50_08765"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEV8027510.1"
                     /translation="MAQIHPTAIIEPGAKLAESVRIGPYCVVGARVELAAEVELLSHV
                     VVAGRTSIGEGTRIFPFASIGHQPQDLKYHGEDSALVIGRRNIIREHVTMNPGTAGGG
                     MVTRIGDDCLFSTSAHVAHDCTVGDHVILTNNATIGGHVVLGDYVVISGLSAVHQFVR
                     VGQHAFVGGMTGVERDVIPYGMVMGDRARLVGLNIVGLQRRGFSREDIQALRAAYDVL
                     FSQESGTLADRAASLPERFPGVKPVRDIVDFIRADNSRGLVQPKTGNGG"
     gene            complement(992..1459)
                     /gene="fabZ"
                     /locus_tag="VGP50_08770"
     CDS             complement(992..1459)
                     /gene="fabZ"
                     /locus_tag="VGP50_08770"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV8027511.1"
                     /translation="MDTTPPGGTAAEIDIQRIMQMIPHRYPFLLIDRVVDVISGESAI
                     GIKNVSINESFFQGHFPQRPVMPGVLVVEAMAQTAAVLVVHTLGPGSERKLVYFMSID
                     DARFRRPVVPGDVIRVHVTKKQHRGNVWKFEGKAKVDDKLVAEATFAAMIMDS"
     gene            complement(1460..2482)
                     /gene="lpxD"
                     /locus_tag="VGP50_08775"
     CDS             complement(1460..2482)
                     /gene="lpxD"
                     /locus_tag="VGP50_08775"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEV8027512.1"
                     /translation="MADPRFFRVAGPFTAAEIARRTGAVLGGAADGDRVLRDVAALDA
                     AGPDALSFLDNRKYVPAFRQTRAGAAFVLPALAGEAPPGLTLLMTEQPYRGFALAAQA
                     FYPEAAPEPGIAASAVIDPTARLDEGCAVEANAAIGPRVEIGRRCHIGANSVIGAGCV
                     LGDDVRVGPNVSISHSIIGSRVRLYPGARIGQDGFGFAPDPGGHVKIPQLGRVIIEDD
                     VEIGANSAIDRGAGLDTVIGAGSMIDNLVQIGHNVQMGRGCVLCGQAGIAGSTRLGDY
                     VFIAAQGGLAGHLSIGSAARIGAQAGVMRDVPPGETVIGAPAVPQKEFFRQVATLQRL
                     ARKKGE"
     gene            complement(2485..3090)
                     /locus_tag="VGP50_08780"
     CDS             complement(2485..3090)
                     /locus_tag="VGP50_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEV8027513.1"
                     /translation="MKARHVLGIAVLLVSALLPAARLWAQQPLAPLILIVDMQQILQD
                     AKAAKGVQSVISQQYNVYSKEVAQQEDELQKARAELERQRTVLTPDAFSLRARELQQR
                     YDDLDQTVQGRRQALQQSLNEAMGKVRNSALEVVAGIVQERRANLVIEKQAIVFEAEG
                     LDVTPEAIERLDQKLSSVPVNLPKPEGEAAKSGAAASDKKN"
     gene            complement(3467..>4937)
                     /gene="bamA"
                     /locus_tag="VGP50_08785"
     CDS             complement(3467..>4937)
                     /gene="bamA"
                     /locus_tag="VGP50_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEV8027514.1"
                     /translation="VDLTSQLTAVSAQDLKALVTVHEGDWYNADGVDKTISALTDALG
                     NRGYAFVKIEPHTKRNADTHTIDVSFDVGEGPRVYVERVDIAGNVRTLDKVIRREMRL
                     IEGDAFNTSRLQRSEQRIKNLGYFKKVDVTTNPGSSPDKTIIGVNVEEQSTGEFSVGA
                     GFSTAEGILGDVSIRERNLLGSGQDLRIGATIAQLVQQVDLSYTEPYFLDRNLAAGFD
                     LFEINQDNQLIGQFNQFTLGGTLRLGYQINEALRQTLKYTLRKDTINDVQSTASPFIQ
                     EEAGTRNTSAVGQVLLYDKRDNRLDPSGGFFAQLSTDLAGLGGDVRYGRVVLSGGYFY
                     SVAPTWVLSFTGEGGYIHGIDDIVRIEDRFFVGGDNLRGFAVGGIGPRDLTTGDALGG
                     NTYYVGSVTLGFPLGLPPELGITGRVFSDFGSLFSIDQSGANTTDTLADPNAIRVSAG
                     FGVSWRSPLGPIKLDLALPIIKQPFDKRQIFLVGFGTRF"
BASE COUNT          823 a         1614 c         1655 g          845 t
ORIGIN      
        1 ctctcccgcg agagcgggag agggagggac ccaccgcgca gcgatgggag ggtgagggag
       61 cgcttcagaa ttgagtgctt cgccagcaac tgccccctca tcctcccgtc gcttcgcgac
      121 gggcccctcc ctctcccgct ctcgcgggag agggattgcg agcggctctt cgaaacgcta
      181 cccgccattc cccgtcttcg gctggacgag gccgcgcgaa ttgtcggcgc ggatgaaatc
      241 gacgatgtcg cgcaccggct tgacgccggg aaagcgctcc ggcagcgatg ccgcgcggtc
      301 ggccagcgtg ccgctctctt gcgagaaaag gacgtcatag gcggcgcgaa gcgcctggat
      361 gtcctcgcgt gagaaaccgc ggcgctgtag tccgacgatg ttgaggccca cgagccgcgc
      421 gcgatcgccc atgaccattc cataagggat gacgtcgcgc tcgacgccgg tcatgccgcc
      481 gacgaaggcg tgctgtccga cgcggacaaa ttggtggacg gcggaaagac cgctaatcac
      541 cacataatcg ccgagaacga catgcccgcc gatggtcgcg ttgttggtca ggatcacgtg
      601 atcgccgacg gtgcagtcat gcgccacatg ggcgctcgtg gaaaagaggc agtcgtcgcc
      661 gatgcgtgtc accatgccgc caccggccgt gccgggattc atcgtgacat gctcgcggat
      721 gatgttacgg cggccgatga cgagagccga atcctcaccg tggtacttca agtcctgcgg
      781 ctgatggccg atcgaggcga acgggaagat acgcgttccc tcgccgatgc tggtgcgtcc
      841 ggcgaccacg acatgggaga gaagctcgac ctcggcggca agctcgaccc tggcgcccac
      901 gacgcaataa gggccgatgc ggacgctctc ggcgagcttg gcacccggct cgatgatggc
      961 ggtcggatgg atctgcgcca cggggcgcgc ctcagctgtc catgatcatc gcggcgaagg
     1021 tcgcctcggc gacgagctta tcatcgacct tggctttgcc ctcgaacttc cagacattgc
     1081 cgcgatgctg cttctttgtg acatggacgc ggatcacgtc gccggggacg accggacggc
     1141 ggaaacgcgc gtcatcgatg ctcatgaaat agacgagctt gcgttcggag cccgggccaa
     1201 gcgtatgcac caccagcacg gccgccgttt gcgccatcgc ctcgacgacg aggacgcccg
     1261 gcatcaccgg gcgctgcggg aagtggccct ggaagaagga ctcgttgatc gatacgttct
     1321 ttatgccgat ggcgctctcg cccgagatca cgtcgaccac gcgatcgatc agcaggaacg
     1381 ggtagcggtg cgggatcatc tgcatgatcc gctggatgtc gatctctgcg gccgtcccgc
     1441 ccggcggggt cgtgtccatc tactcgccct tcttgcgcgc caggcgctgc agggtcgcga
     1501 cttggcggaa gaattccttc tgcggcaccg ccggcgcacc gatcacggtc tcgcccggcg
     1561 ggacgtcgcg catgaccccc gcctgcgcgc cgatacgggc ggcgctgccg atgctgaggt
     1621 ggccggccaa cccgccttgc gcggcgatga agacgtaatc gccgagccgg gtactgccgg
     1681 cgatccccgc ctgcccgcac agcacgcagc cgcggcccat ctgtacgttg tgcccgattt
     1741 gcaccagatt atcaatcatc gagcccgcgc cgatcacggt atcgaggccg gcgccccggt
     1801 cgatggcgct gttggcgccg atctcgacgt cgtcctcgat gatcacgcgc cccagctgcg
     1861 ggatcttcac atgcccgccg gggtcggggg cgaagccgaa gccgtcctga ccgatgcgcg
     1921 cgcccggata aagtcgcacc cgcgagccaa tgatgctgtg gctgatgctg acattggggc
     1981 cgacgcgtac gtcgtcgccg agaacgcatc cggcgccgat cacgctgttg gcgccgatat
     2041 ggcagcgccg gccgatctcg acgcgggggc cgatcgcggc attggcctcg acggcacagc
     2101 cctcatcgag acgcgccgtc ggatcgatca ccgcgctcgc cgcgatccct ggctcgggcg
     2161 ccgcttcagg atagaaggcc tgagccgcca gggcgaaccc gcgatagggc tgctcggtca
     2221 tgagcagcgt caagccggga ggcgcctccc ccgccagcgc cggcaggacg aaggccgcgc
     2281 cggcgcgcgt ctggcggaat gctggcacat acttacggtt gtcgagaaag ctcagcgcgt
     2341 ccggaccggc cgcatcgagt gccgcaacgt cgcgcaggac gcggtcgcca tcggcggcgc
     2401 cgcccaggac ggcgccggtc cgccgggcga tctcggccgc agtgaaaggg cccgcgacgc
     2461 gaaagaagcg cggatcggcc atctctagtt cttcttgtcg gacgcggcag cgccgctttt
     2521 cgccgcttcc ccctcgggct tcgggaggtt cacgggcacg gaggaaagct tctggtcgag
     2581 ccgctcgatc gcttccggcg tcacgtcgag gccctccgcc tcgaacacga tcgcctgctt
     2641 ctcgatcacc agattggcgc gacgctcctg gacgatgccg gcgacgacct caagggccga
     2701 atttcgtacc ttgcccatgg cttcgttgag gctctgctgg agcgcctggc gccgcccttg
     2761 aaccgtctgg tcgaggtcgt catagcgctg ctgcagctca cgcgccctga ggctgaaggc
     2821 gtcgggcgtg aggaccgtgc gctggcgctc gagctcggca cgcgccttct gcagttcgtc
     2881 ctcctgctga gcgacttcct tgctgtagac attatactgc tgactgatga ccgattgtac
     2941 gcccttggcg gccttcgcgt cctgcaggat ctgctgcatg tcgacgatga ggatgagcgg
     3001 cgcgagcggt tgctgggccc agaggcgcgc cgcgggaagc agcgcagaca ccagcaggac
     3061 ggcaatgccg aggacgtgac gtgctttcat cggacgcaag ctcctccgaa acacgatatc
     3121 agatcgtccc atccggccag gagcctgtct gagcaatgga acgggtctgc gttgcacggg
     3181 gatggggcgc ggttaggagc ggcggctctc ggcgaagctt cgcttcgcct gacgccgaca
     3241 gcgcaagctt cgcttgcgcg agaggcgcca gcgatgcctt gcgcatcggt ggcaacggcg
     3301 cgacgcggca gcgccccatc cccgcgcaac cctccgggac attggcattt gccgctgggc
     3361 gtcgtcgctc gtcgcttacg atgcgcatgg catcgctgcg ctcctcgctc ctagcccagc
     3421 ggcaaatgcc aatgtcgcag acccgttcca ttgctcagac aggctcctaa aacctcgtgc
     3481 cgaagccgac caggaagatc tgccgcttgt cgaagggctg cttgatgatc ggcagcgcca
     3541 agtcgagctt gatggggccg agcggcgagc gccaggagac gccgaagccc gccgagacgc
     3601 gaatcgcgtt cggatcggca agtgtgtcgg tcgtgttcgc gcccgactga tcgatgctga
     3661 acaagctgcc gaagtccgag aagacgcggc cggtgatccc gagttccggc ggcagcccca
     3721 gcgggaagcc cagcgtcacc gacccgacat agtaagtgtt gccgccgagc gcatcgccgg
     3781 tcgtcagatc gcgcgggccg atgccaccca ccgcgaaccc gcggagattg tcgccgccga
     3841 cgaagaaccg gtcctcgatc cgcacgatgt cgtcgatgcc gtggatatag ccgccttcgc
     3901 cggtgaagct cagcacccag gtcggcgcga ccgagtagaa atagccgccc gaaagcacca
     3961 cacggccata gcgcacgtcg ccgccgaggc cggccagatc ggtggagagc tgggcgaaga
     4021 agccgccgct cggatcgagg cggttgtcgc gcttgtcgta gagcagcacc tggccgacag
     4081 ccgaggtgtt gcgcgtgccg gcctcctcct gaatgaaggg cgacgccgtc gactggacgt
     4141 cgttgatggt atccttgcgc agcgtgtatt tgagggtctg ccgcagcgcc tcgttgatct
     4201 gatagccgag acgcaaggtg ccgcccagcg tgaactggtt gaactggccg atgagctggt
     4261 tgtcctggtt gatctcgaag agatcgaagc cggcggcgag attgcggtcg aggaaatacg
     4321 gctcggtgta gctcagatcg acctgctgga cgagctgggc gatggtcgcg ccgatgcgca
     4381 ggtcctggcc gctgcccaag aggtttcgtt cacggatcga gacgtcgccc aggatgcctt
     4441 ccgccgtcga gaagccggcg ccgaccgaga actcgccggt cgactgctcc tcgacattga
     4501 cgccgatgat cgtcttgtcg gggctgctgc ccgggttggt cgtgacgtcg accttcttga
     4561 agtagccgag attcttgatg cgttgctcgg agcgctgcag gcgcgaggta ttgaaggcgt
     4621 cgccctcgat cagtcgcatc tcgcggcgga tcaccttgtc gagggtgcgc acattgccgg
     4681 cgatgtcgac gcgctcgaca tagacccgcg gcccttcgcc gacatcgaaa gagacgtcga
     4741 tggtgtgcgt gtcggcgttg cgcttggtgt gcggctcgat cttgacgaac gcgtagcccc
     4801 ggttgccgag cgcgtcggtg agcgcgctga tcgtcttgtc cacgccatcg gcgttgtacc
     4861 aatcgccttc gtgcaccgtc accagcgcct tcaggtcctg ggcgctgacg gccgtgagct
     4921 ggctcgtgag atcgacc
//
LOCUS       DASYNS010000282         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3656165, whole genome shotgun sequence.
ACCESSION   DASYNS010000282 DASYNS010000000
VERSION     DASYNS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3656165"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..2367
                     /locus_tag="VGP50_08790"
     CDS             178..2367
                     /locus_tag="VGP50_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEV8027515.1"
                     /translation="MAATLSEYGPTPDEPISPFYIPASSAAGIARANVLKHGDCFAVL
                     DDFGNAQATGPAAEGLFFEDTRYLSQLALTLDGMRPLLLSSTVSQDNALLEADLTNPD
                     LVEDGRLRSARDTVHIWSTTTLGEDALFARLELRNFAMTSAGFRLAFHFEADFCDIFE
                     LRGSVRRQRGRLLRDERTSDGVVLGYLGLDRAIRRTRLIAEPSPDRVEPRQAEWLIVL
                     APGATQTIQLTARCERDERPSRGKSRQASLATVAQGRVDYARRAASLTSSNEAFNELL
                     SRSRADLDMLTTETPQGLYAYAGIPWFSTAFGRDGLITALECLWLDPTLAAGTLRFLA
                     ARQATSLDPKADAEPGKILHETRKGEMSMLGEVPFRCYYGSVDATLLFIVLAAAYHAR
                     TGDLDLVRTLWPNIEAALGWMARYGDSDGDGFMEYDRKSVNGLVNQGWKDSGDAIFHA
                     DGRLAEAPIALAEVQGYAHAAYVGAAKLAAALGQEQRAAELRALGERLGQRFEAAFWS
                     EELGTYAIALDGQKQPCCVRSSNAGQVLFGGICSAERAERVAANLMTPEFFSGWGIRT
                     IAESEARYNPMSYHNGSVWPHDNALIGMGFGRYGLRRPLIALLTGLFEAALVLELKRL
                     PELFCGFTRRHATGPTSYPVACNPQAWSSASVIAVLGALLGIAFDPAGRRVAFHQPAL
                     PPWLDTLKLTNLQLGGASLDLLLERRGERIGFDVLARRGDIELAVMP"
     gene            2364..>3425
                     /locus_tag="VGP50_08795"
     CDS             2364..>3425
                     /locus_tag="VGP50_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027516.1"
                     /translation="MSTDSDATDLPRLASRGPVLFLWHYVRRRLARHLVVLLAVLTAV
                     SCAVGAQYAVKNLVDVLVAGDPSDWELWGAVAILLGLVAGDNLLWRLAGWIATSAFVA
                     VGGDLRLDLFEHLSGHGTHYFADRFPGALAGRITTVATAASTIENSLTWSTIPPATAV
                     IISIAMLGSIDWRITLVLAIIVVALGALIARLASRGQYLHRRFAGRAAAVTGSLTDVV
                     SNIGLVRAFGAARREKQRLSEEIGREMTAQRESLLALERLRLLHALAVFAVTAGILAW
                     SVSFWRLGLVTAGDVVLTTSLGFTVLHASRDLAMAMVELVQHFAKLGEAVQVLGLPHE
                     MEDSSDAKPLIRLGGSVSFL"
BASE COUNT          461 a         1229 c         1149 g          586 t
ORIGIN      
        1 cgaggaccag gccgtggcgg cggtgagcaa gctgcattcc ctcgaccgcg cccgcgtgcg
       61 gcgcatcttc gaggagcgct tcaccgcgcg gcgcatggcg gacgactatc tcgcggtcta
      121 tcgcggcctg atcgcgcgcg agcggccgct cgaacggccg ctcaaagccg tcgtgtgatg
      181 gcggcgacgc tttcggagta cgggccaacg ccggacgagc cgatctcgcc gttctacatc
      241 cctgcgagct cggccgccgg catcgctcgc gccaatgtgc tgaagcacgg cgattgcttt
      301 gccgtgctcg acgatttcgg caacgcgcag gcgaccggac ccgccgccga ggggctgttc
      361 ttcgaggaca cgcgatacct ctcgcagctc gcgttgacgc tcgacgggat gcggcctttg
      421 ctgctgtcct cgacggtcag tcaggacaac gctctcctcg aggcggacct caccaacccc
      481 gacctcgtcg aggacggccg gctgcgctcc gcgcgcgata ccgtccatat ctggagcacc
      541 acgacgctcg gcgaggacgc gttgttcgcg cggctggagc tgcgcaactt cgccatgacc
      601 agcgccggct ttcgcctcgc cttccacttc gaggccgatt tctgcgacat cttcgagctg
      661 cgcggcagcg tgcggcgcca gcgcggccgg ctgctccgcg acgagcgcac gagcgacggc
      721 gtggttctcg gctatctcgg cctcgaccgg gcgatccgcc gcacgcggct catcgccgag
      781 ccgtcgccgg accgggtcga gcctcgccag gcggaatggc tgatcgtcct tgcaccgggc
      841 gccacccaaa ccatccagct gacggcgcgc tgcgagcgcg acgagcggcc gtcgcgcggg
      901 aaatcgcgcc aggcgagcct tgccaccgtg gcccagggac gcgtcgacta cgcgcgccgc
      961 gccgcgtcgc tcaccagcag caacgaagcg ttcaacgagc tgctgagccg ctcgcgcgcc
     1021 gatctcgaca tgctgaccac cgagacgccg caagggctct atgcctacgc cggcattccc
     1081 tggttctcca ccgccttcgg tcgcgacggg ctcatcaccg cgctcgaatg cctctggctc
     1141 gacccgacgc tggcggccgg gaccttgcgc tttctcgcgg ctcgccaggc gacgtcgctc
     1201 gatccaaagg ccgacgccga gcccggcaag atcctgcacg agacgcgtaa gggcgagatg
     1261 tccatgctcg gcgaggtgcc gttccgctgc tactacggca gcgtcgacgc cacgctgctc
     1321 ttcatcgtgc tcgccgccgc ctatcacgcg cgcaccggcg atctcgatct cgtccgcacg
     1381 ctctggccga acatcgaggc ggcgctcggc tggatggccc gctacggcga ctccgatggc
     1441 gacgggttca tggaatacga ccggaaatcg gtgaacgggc tggtcaatca aggctggaag
     1501 gacagcggcg acgcgatttt ccacgccgac ggcaggctgg ccgaggcgcc catcgctctg
     1561 gccgaggtcc agggctacgc ccatgccgcc tatgtcggtg cagcgaagct cgcggcggcg
     1621 ctgggccagg agcagcgggc ggcggagctg cgggcgctcg gcgagcggct ggggcagcgt
     1681 ttcgaggccg ccttctggag cgaggagctc ggcacctacg cgatcgcgct cgacgggcag
     1741 aagcagccct gttgcgtgcg cagctccaat gccgggcagg tgctgttcgg cggtatctgc
     1801 tcggccgagc gcgcggagcg tgtcgccgcc aatctgatga cgcccgaatt cttttccggc
     1861 tggggcatcc gcaccatcgc cgaaagcgag gcgcgctaca acccgatgtc ctaccacaac
     1921 ggctcggtct ggccgcacga caacgcgctc atcggcatgg gctttggccg ctatggcctg
     1981 agacggccgc tcatcgcgct gctcaccggc ctcttcgagg ccgcgctcgt gctcgaattg
     2041 aagcggttgc ccgagctctt ctgcggcttt acgcgccggc acgcgacggg tccgaccagc
     2101 tatcccgtcg cgtgcaatcc ccaggcctgg tcctcggcct cggttatcgc cgtgttgggc
     2161 gcgctgctcg gcatcgcctt cgatccggcg gggcgccgcg tcgctttcca ccagccggcg
     2221 ctgccgccct ggctcgatac gctgaagctc accaatctcc agctcggcgg ggcctcgctc
     2281 gatctcctgc tcgagcggcg cggcgagcgg atcggcttcg atgtcctggc ccgccgcggc
     2341 gacatcgagc tcgcggtgat gccgtgagca ccgacagcga cgcaacggat ctgccgcgtc
     2401 tcgcctcgcg cggccccgtc ctttttctct ggcactatgt gcgccgccgc ctggcccgcc
     2461 atctcgtggt gctgctggcg gtgctgacgg ccgtgagctg cgccgtcggc gcgcaatatg
     2521 cggtgaagaa cctcgtcgat gtgctggtcg cgggcgaccc cagcgactgg gagctctggg
     2581 gcgcggtcgc catcctgctc ggcctcgtcg ccggcgacaa tctgctgtgg cggcttgccg
     2641 gctggatcgc gacgtcggcg ttcgtcgccg tcggcggtga tctgcgcctc gacttgttcg
     2701 agcacctctc gggtcacggt acgcattatt ttgccgatcg cttccccggc gcgctcgccg
     2761 gccgcatcac gaccgtcgcc acggcggcat cgacgatcga gaactcgctg acctggagca
     2821 ccattccgcc ggcgacggcg gtgatcatca gcattgccat gctgggctcg atcgattggc
     2881 ggatcacgct cgtgctggcg atcatcgtcg tcgcgctcgg cgcactcatc gcgcggctgg
     2941 cgtcgcgcgg ccagtatctg catcgccgct tcgccgggcg cgccgccgcc gtcaccggca
     3001 gcctcaccga cgtcgtgtcc aatatcggcc tggtccgtgc cttcggcgcc gcgcggcgcg
     3061 agaagcagcg gctttccgag gagatcggcc gcgagatgac ggcgcagcgg gagagcctgc
     3121 tcgccctcga gcggctgcgg ctgcttcacg cgcttgctgt ctttgcggtg accgccggca
     3181 tcctcgcttg gtcggtgagc ttctggcgcc tgggcttggt gaccgcgggc gacgtcgtgc
     3241 tgacgacgtc gctgggcttc accgtgctcc acgcctcgcg cgatctcgcc atggcgatgg
     3301 tcgagctggt gcagcatttc gccaagctcg gcgaagcggt gcaggtgctc ggattgccgc
     3361 acgagatgga agactcgtcc gacgccaagc cgctcatccg cctcggcggc tccgtcagct
     3421 tcctc
//
LOCUS       DASYNS010000283         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_534271, whole genome shotgun sequence.
ACCESSION   DASYNS010000283 DASYNS010000000
VERSION     DASYNS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534271"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VGP50_08800"
     CDS             <1..207
                     /locus_tag="VGP50_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8027517.1"
                     /translation="PGLKKKPSTSELLDWLKLLMVEDVAPEVLRTRDPAKLIPPLHGA
                     LLKNEQDVHLFERLAFLNRRERGG"
     gene            207..1382
                     /locus_tag="VGP50_08805"
     CDS             207..1382
                     /locus_tag="VGP50_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV8027518.1"
                     /translation="MFAGFFLELREAKVPVSLREYLTLMEAMKQNLAAYSVDDFYYLS
                     RAALVKDERNLDKFDRVFGHHFKGLETVAEPGAAIPEEWLRKLAEKLLTEEEKKQIEA
                     LGGWEKLMETLRQRLAEQKERHQGGSKWIGTAGTSPFGAYGYNPEGVRVGQEANRNNR
                     AVKVWDKREFKNLDDRIELGTRNIKVALRRLRQFAREGAADELDLDDTIRSTARNAGW
                     LDIKMVPERHNAVKVLLLLDIGGSMDGHVRTCEELFSAARAEFKHLEYFYFHNCPYEA
                     MWRDAWRRHVERTPTMQLLHTYDASYKLIFVGDASMSPYEIMQPGGSVEHWNEEAGEV
                     WMRRLLATYSKAIWLNPVPERYWEGAPSIQMMRQLMEGRMFPLTLDGLDRATRRLTR"
     gene            complement(1447..>1731)
                     /locus_tag="VGP50_08810"
     CDS             complement(1447..>1731)
                     /locus_tag="VGP50_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEV8027519.1"
                     /translation="LSTAEIGRRLGVSKNAVVGKAHRLSLDPRPSPLKRPPVRRVVGI
                     AGPTCSWPSGHPGERDFHFCGKRPVPGKPYCAEHAELAYVKPKVEKSNAA"
BASE COUNT          327 a          561 c          566 g          277 t
ORIGIN      
        1 cccggcctca agaagaagcc gtcgacgtcg gagctgctgg actggctgaa gctcctcatg
       61 gtcgaggatg tcgcgcccga ggtgctgcgc acccgcgacc cggcgaagct cattccgccg
      121 ctccatggcg cgctcctcaa gaacgagcag gacgtgcatc tcttcgagcg cctcgccttc
      181 ctcaatcggc gcgagcgggg cggctgatgt tcgccggctt cttcctcgag ctgcgcgagg
      241 ccaaggtgcc cgtgtcgctg cgcgagtatc tgactctgat ggaggcgatg aagcagaacc
      301 tcgccgccta cagcgtcgac gatttctact atctatcccg cgccgcgctg gtgaaggacg
      361 agcgcaatct cgacaagttc gaccgcgtgt tcggccatca tttcaaaggc ctcgagaccg
      421 tggccgagcc cggcgccgcg attcccgagg agtggctcag aaagcttgcg gagaagctgc
      481 tgaccgagga ggagaagaag cagatcgagg cgctgggcgg ctgggagaag ctgatggaga
      541 ccttgcgcca gcgcctcgcc gagcagaaag agcgccatca gggcggcagc aaatggatcg
      601 gcactgccgg cacctcgccc ttcggcgcct atggctacaa tcccgagggc gtgcgcgtcg
      661 gccaggaggc aaaccgcaac aaccgggcgg tcaaggtgtg ggacaagcgc gagttcaaga
      721 acctcgacga ccgcatcgag ctgggcacgc gcaacatcaa agtcgcgctg cgacgcctgc
      781 ggcaattcgc gcgcgaaggc gccgccgacg agctcgatct cgacgacacc atccgctcga
      841 ccgcgcgcaa tgccggctgg ctcgacatca agatggtgcc ggagcggcac aatgcggtga
      901 aggtgctgct gctcctcgac atcggcggct cgatggacgg ccacgtccgc acctgcgagg
      961 aactcttctc ggcggcgcgc gccgagttca agcacctcga atatttctat tttcacaatt
     1021 gcccctacga ggcgatgtgg cgcgacgcgt ggcgccgcca tgtcgagcgc acgccgacga
     1081 tgcagctgct gcacacctat gacgcgagct acaagctcat cttcgtcggc gacgccagca
     1141 tgagccccta cgagatcatg cagcccggcg gcagcgtcga gcattggaac gaagaagcgg
     1201 gcgaggtctg gatgcgccgc ctgctcgcga catattcgaa agcaatctgg ctcaatccgg
     1261 tgccggagcg ctactgggaa ggcgctccgt cgatccagat gatgcgtcag ctcatggaag
     1321 gccgcatgtt cccgctcacc ctcgacgggc tcgaccgggc gacgcggcgg ctgacaagat
     1381 agttgtcagt tatcagtgcg tggtggcaca tgactgacga ctgataactg aggactgacc
     1441 actttcctaa gccgcgttcg atttctcgac cttcggcttg acgtaagcga gctcggcgtg
     1501 ctccgcgcaa tagggcttac ccggcaccgg ccgcttgccg cagaaatgga agtcccgctc
     1561 gcccggatgg ccgctgggcc aggagcaggt cgggccggcg atgccgacga cgcggcgcac
     1621 cggcggccgc ttcaggggcg acgggcgcgg atcgagcgac aggcgatgcg ccttgcccac
     1681 cacggcattc ttgctcacgc ccaggcgccg accgatctcg gcggtcgaca g
//
LOCUS       DASYNS010000284         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1159140, whole genome shotgun sequence.
ACCESSION   DASYNS010000284 DASYNS010000000
VERSION     DASYNS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159140"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VGP50_08815"
     CDS             complement(<1..418)
                     /locus_tag="VGP50_08815"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEV8027520.1"
                     /translation="MMMDLEALVRRAAGGDVEAFVELTRRFQHFAFGSALALIGDFQE
                     AENAAQEAFVAAWSALPSLADPAAFPGWLRGIVRHQAFRVLRRRRPEPLPLSAAEGIA
                     SDEASPEMRLDERRRAAAVLAAMAELPAALREPATLF"
     gene            complement(496..1017)
                     /locus_tag="VGP50_08820"
     CDS             complement(496..1017)
                     /locus_tag="VGP50_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEV8027521.1"
                     /translation="MAIQAQYLGMPLDVSELDVENLEYFRYCAQHEFHLQACARCGLL
                     RYPPTTACPWCAHPESRWVPVEGSGTVHSYTEVHHAIQPAFRSRLPYLVVLVELDTQK
                     GKPSAEEALRIVGNLVTPDGALAPAEMVRRVGIGSRLRMVFSDVAPGLALPQWTIDET
                     RPQPATVWRYPQE"
     gene            complement(1033..2283)
                     /locus_tag="VGP50_08825"
     CDS             complement(1033..2283)
                     /locus_tag="VGP50_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027522.1"
                     /translation="MNKEVDATTPRYLRDKYAIAGVGETTYTRGSGRTTRALASWAVR
                     NAILDAGLTPADIDGMLSYQSGDSTFAPFVAGDLGIRLNFYMDVFGGGSSTEALIGIA
                     LGVIEAGMCKTVVIFRAMNGYSQVRIGGTGARAVVPVSGDMLHSRAYGWQSAGQMFSP
                     TFMRHMYDYGTRPEQVAAVKAIHSEHAANNPKALYKERVTVEDVLQSRIICKPLHLLD
                     CCVETDNGNAIVVTTAERARDCRHPLVLIRGVVGRCSKPRADMHYQAGPISTVAGRYA
                     REILWPNSGVSPAEIDVTGSYDAFTFTTMLQLEDYGFCKKGEGGDYVSDGTIRLGGKR
                     PNNTSGGHLCEGYTHGINMVIENVRQLRHDADDSCPVDADGRRRHSHDYRAGGCRQVR
                     DVELTANLGWAMPGTGSAMVMRRG"
     gene            complement(2602..>3015)
                     /locus_tag="VGP50_08830"
     CDS             complement(2602..>3015)
                     /locus_tag="VGP50_08830"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8027523.1"
                     /translation="FNKAFAPTRTPADWLTRDPAEVDKYVADPLCGFIATTQTWVDLL
                     DALRQIARPELQALIPKRLPIHIISGSTDPVSDGTKRLQQLLAAYRGAGLERVTHRFY
                     EGARHELFNETNRDEVTRDLILWLDDVIAARADPA"
BASE COUNT          491 a          995 c         1028 g          501 t
ORIGIN      
        1 agaagagggt cgccggctct cggagcgcgg cgggcagctc cgccatcgcc gccagcacgg
       61 cagcggcccg ccggcgctcg tcgagccgca tctccgggga tgcttcgtcc gacgctatcc
      121 cttcggcggc gctcaggggc aacggctcgg gtcgccggcg cctcagcacc cggaacgcct
      181 ggtgccgcac gatgccgcgg agccatcccg gaaacgccgc cggatcggca aggctcggca
      241 acgccgacca cgcggcgacg aaggcttcct gagcggcgtt ctcggcctcc tggaaatcgc
      301 cgatcagcgc cagtgccgaa ccgaaggcaa aatgctggaa ccgccgcgtc agctcgacga
      361 aggcctcgac atcgccgccc gcggcccggc gtaccaaggc ttccagatcc atcatcatcc
      421 cctctcgctc ctaaagtgcc agaaaggggc ggccgccttt aacccggatc aggattaagc
      481 gcgagcgcgg cggcgctatt cctgcggata gcgccagacc gtcgccggct gcggccgcgt
      541 ctcgtcgatc gtccattgcg gcaaggcgag accgggcgcg acgtcgctga agaccatgcg
      601 caggcgcgag cctataccga cgcggcgcac catctccgcc ggggcgagag cgccgtcggg
      661 cgtcacgaga ttgccgacga tgcgcaacgc ctcttccgcg ctcggctttc ccttctgcgt
      721 gtcgagctcg accagcacga ccagataggg aagcctgctc cggaaggccg gctgaatcgc
      781 gtgatgcacc tcggtatagg aatggacggt gccgcttccc tcgaccggga cccagcgcga
      841 ttcgggatga gcacaccacg ggcaggcggt ggtcggcggg taacgcagca ggccgcagcg
      901 cgcgcaggcc tggagatgga attcatgctg cgcgcaataa cgaaaatatt cgagattctc
      961 gacatcgagc tcgctcacat cgagcggcat gccgagatat tgggcttgaa tggccatgga
     1021 tcgtccctcc cctcagccgc gccgcatcac catcgccgag ccggtgcccg gcatcgccca
     1081 gccgagattg gcggtgagct cgacgtcccg cacctggcgg catccgcctg cgcggtagtc
     1141 gtggctgtgc cggcgccgcc cgtccgcgtc caccgggcag gaatcgtcgg cgtcgtggcg
     1201 gagctggcgg acattctcga tcaccatgtt gatgccgtgg gtgtagccct cgcagagatg
     1261 gccgccgctc gtgttgttgg ggcgcttgcc gcccaggcgg atggtgccgt cgctgacata
     1321 gtcgccgcct tcgcccttct tgcagaagcc gtaatcctcg agctgcagca tcgtcgtgaa
     1381 ggtgaaggcg tcgtaggagc cggtgacgtc gatctccgcg ggcgagacgc cggaattcgg
     1441 ccagaggatc tcgcgcgcgt agcggccggc gacggtcgag atgggtcccg cctggtaatg
     1501 catgtcggcg cgcggcttgc tgcagcggcc gaccacgccg cggatcagca cgagcggatg
     1561 gcggcagtcg cgcgcgcgct cggccgtggt cacgacgatg gcgttgccgt tgtcggtctc
     1621 gacgcagcaa tcgaggagat gaagcggttt gcagatgatg cggctttgaa gcacatcttc
     1681 gaccgtcacc cgttccttgt agagcgcctt cggattgttc gcggcatgct cgctgtggat
     1741 cgccttgacc gccgcgacct gctcgggacg cgtgccgtag tcgtacatgt ggcgcatgaa
     1801 ggtcgggctg aacatctggc cggcgctctg ccagccatag gcgcggctgt gcagcatgtc
     1861 gccggagacc ggcaccacgg cgcgcgcgcc ggtgccgccg atgcgcactt gcgagtagcc
     1921 gttcatggcg cggaagatca ccaccgtctt gcacatgccg gcctcgatca cgcccaaggc
     1981 gatgccgatc agcgcctcgg tcgaggagcc gccgccgaac acgtccatgt agaaattgag
     2041 gcggatgccg agatcgccgg cgacgaaggg cgcgaaggtc gaatcacccg attggtagga
     2101 cagcatgccg tcgatatcgg cgggggtgag cccggcgtcg aggatcgcgt tgcgcaccgc
     2161 ccagctggcg agcgcgcgcg tcgtccgccc cgagccgcgc gtgtatgtcg tttcgcccac
     2221 gcccgcgatc gcgtatttat cgcgcagata tctgggcgtc gtggcgtcga cttccttgtt
     2281 catcggcacc gctgcttctc ccctttttcc gtgaaccgcg cgcgtcctag catgaccgga
     2341 cggcgcgcgc caatcgcgct tcgatcgcgc gcattgcgcg aaaaggtccg acaaattccc
     2401 ggcgaaaggc gttgccccga atgggggcga gcatatgctg acccgcggtc cattgtggca
     2461 ctcgggagag aagcaccaat gggcgtcgcc cgtctcaagg ggggagcatt atttcgagtc
     2521 agccccggtc aaaaaagccc cctcccccct tgagcgcgag cgtcggatgc ggcgggacca
     2581 gcgccgctgc agtcgcgtcc ctcacgcggg gtcggcgcgc gccgcgatca cgtcgtcgag
     2641 ccagaggatc aggtcgcgcg tgacctcgtc gcggttggtc tcgttgaaga gctcatgccg
     2701 ggcaccctcg tagaagcgat gcgtcacccg ttcgaggccg gcgccgcgat acgcggcgag
     2761 aagctgctgg agccgtttgg tgccgtcact gacgggatcg gtgctgccgc tgatgatgtg
     2821 gatgggcaga cgcttcggaa tgagcgcctg gagttccggc ctggcgattt gccgcaaggc
     2881 atcgaggaga tcgacccagg tttgtgtcgt ggcgatgaag ccgcagagcg gatcggcaac
     2941 atatttgtcg acctccgccg gatcgcgcgt cagccagtcc gccggcgtgc gcgtcggcgc
     3001 gaaagccttg ttgaa
//
LOCUS       DASYNS010000285         4673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5059115, whole genome shotgun sequence.
ACCESSION   DASYNS010000285 DASYNS010000000
VERSION     DASYNS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5059115"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..742)
                     /locus_tag="VGP50_08835"
     CDS             complement(326..742)
                     /locus_tag="VGP50_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEV8027524.1"
                     /translation="MKFLLDTNVISEIRKRDRAHQNVVDWVARTPIEEIGTSVLVLAE
                     IRHGIELKRRNDPEQAKSLDRWFSRMRIRLGDRVLPIDEPVAEAWALLGIPDPLPLID
                     GLLAATAKVHDLTLVTRNVADIAVVGVSLLDPFSVG"
     gene            complement(739..975)
                     /locus_tag="VGP50_08840"
     CDS             complement(739..975)
                     /locus_tag="VGP50_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027525.1"
                     /translation="MRVEVQAPKKDASLIRALAETLRGGTEKAEALRSSLAKALMHPE
                     AKTAFDIFGSELPDETFAGVFDRLRQGSWREVDL"
     gene            complement(1192..1267)
                     /locus_tag="VGP50_08845"
     tRNA            complement(1192..1267)
                     /locus_tag="VGP50_08845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1232..1234),aa:Arg,seq:tct)
     gene            complement(1387..1683)
                     /locus_tag="VGP50_08850"
     CDS             complement(1387..1683)
                     /locus_tag="VGP50_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEV8027526.1"
                     /translation="MAQARIYQPVKTAMQSGRAGTRKWRLDYEQATPRLPDPLMGWTS
                     AGDTLNQIHLHFDTLEEARAFAEKHGLDYVVIATHERIFKPKAYADNFRTDRPR"
     gene            complement(1722..1798)
                     /locus_tag="VGP50_08855"
     tRNA            complement(1722..1798)
                     /locus_tag="VGP50_08855"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1762..1764),aa:Pro,seq:tgg)
     gene            1939..2601
                     /locus_tag="VGP50_08860"
     CDS             1939..2601
                     /locus_tag="VGP50_08860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8027527.1"
                     /translation="MTKRSIGLSAKLHDYILSVSLREPEILRQLREETAKLPLAVMQV
                     APDQGQFMQLLVRLTGARSYLEIGTFTGYSTLAVALALPPDGRILACDINPKTTEVAQ
                     RYWTAAGVASKIELVLAPALETLDKQLTQGQAGRFDLAFIDADKENYDGYYERALRLV
                     RPGGLILLDNVLWDGAVADPKKNDADTKALRAVNKKIQGDERVDVSMLPLADGLTLAR
                     KR"
     gene            complement(2769..3233)
                     /locus_tag="VGP50_08865"
     CDS             complement(2769..3233)
                     /locus_tag="VGP50_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEV8027528.1"
                     /translation="MLRLRIAALMLLVSLAPAAAARADEVASFDKSTITIDTIAGPHH
                     FAVELALTPEQQELGLMFRPSLAPDAGMLFDFGATKPASFWMKNTLIPLDMLFITADG
                     RVADIHEHAVPLSEDVIQSSVPVRAVLEVKGDTVAQLGIRVGDIIHNPIFHN"
     gene            3400..4656
                     /locus_tag="VGP50_08870"
     CDS             3400..4656
                     /locus_tag="VGP50_08870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEV8027529.1"
                     /translation="MTDLLPTTAELGTPAPARGFMGALRSGSWLDRRRIVGYCVALLI
                     FEVVAFCIAVVATHGWVVPLNEPTTTDFASFYAAGVLANAGTPGLAYDSVRHFAVEHQ
                     VTGGVNYQFFFYPPVYLLVCAGLARLPYMVAFILFEAATAALYLVVARGILGEKNGAA
                     SLVLLSFPAVFWTIGLGQNALLTASLFGAATLLVDRRPVLAGLLFGALCYKPHVGLLI
                     PLALAGGGRWRCFAAAAASASLLVLLSLALFGVQTWIDFLAAVTNAPDTYQSGRISLF
                     AMISPFGAVRLMGGGPVLAYGAQLAAVAAMAGIVAVIWRLRLSEPVRAAILAAATPIA
                     MPVALIYDMMLAGIAMAWLARAARATGFLPWEKMALAGLFAGALLSFTLSGPLHIPFA
                     LLSLLGLVAVIVARARWELTRARDPA"
BASE COUNT          740 a         1558 c         1499 g          876 t
ORIGIN      
        1 ctgccgctgc cggcggcgtt ctgcacggcc gcgcgatcct gcgcctcggt atcggccgcg
       61 agcgcgggca gcgtgcgcgg cgccagtgtg cagatggcta aggcactgac gattaacgtg
      121 agcaaagggc gcatcggcag cctcatcgga atagatcatt cccgcgaagc cacagtgtcg
      181 catgtcggtc gttgaggact ttctaatgct caggcgctca cgcgctcgcc ccgcgccggt
      241 ttggccgcgt tcgacggcaa ttatcttccc ggaaacgggc gcgatcggcg cgagtattcg
      301 cgcggcgttc ttgctccgcg acctgtcagc cgacagaaaa tggatcgagc agcgacacgc
      361 cgacgacagc aatgtctgcg acatttcggg tcaccagcgt caggtcatgc actttggctg
      421 tggccgccag gagcccgtcg atcagcggca atggatcagg aattcccaag agcgcccagg
      481 cttccgcgac cggctcatcg ataggcagga cgcgatctcc gagccggatc cgcattcgcg
      541 agaaccatcg atcgagagat ttagcctgtt ccgggtcgtt ccgtcgtttc aactcgatgc
      601 catgacggat ttcggcgagc accaagacgc tcgtcccgat ctcctcgata ggcgtccggg
      661 cgacccaatc gacaacgttc tgatgggctc ggtcgcgctt gcggatttcg gaaatgacgt
      721 ttgtatcgag caggaatttc aaagatcgac ctcgcgccaa ctcccctggc gcaagcgatc
      781 aaaaacgccg gcgaaggtct cgtcgggaag ctccgagccg aagatatcga aggcggtctt
      841 cgcctcggga tgcatcagcg ctttcgccag cgaggatcgg agcgcctcag ccttttcggt
      901 cccgccgcgc agcgtctcgg ccaacgctcg gatcaggctc gcatctttct tgggcgcctg
      961 aacctcaacg cgcacgagac ctcgtgctgc cgcgcgacgc cggtgcgtac gggtcgcctt
     1021 ccgttgggat ttggtggcca tggtcttgct cgacgtcgca tggggctgct gttccggtta
     1081 tataaccgga gatactgtaa agcccaagcg aattctttcc cggagcacag cgcccgggtt
     1141 agaggccgac gtctaaaaat cggcgggttc tggcctagtt ttgtaccgaa gtggcgcgcc
     1201 ctactggaat cgaaccagtg acctaccgct tagaaggcgg ttgctctatc cgctgagcta
     1261 agggcgcccc gtggctccga cattagcaag cgggatgagc ggcggcgagg tcccctcacc
     1321 ctcccactgc tacgctgacg cttcgcagtg ggcccctccc tctcccgcaa tgcgggcgag
     1381 ggtttttcag cggggacggt cggtgcggaa gttgtcggcg taggctttgg gcttgaagat
     1441 gcgctcatgc gtcgcgatga cgacgtagtc gaggccgtgc ttctcggcga aggcgcgcgc
     1501 ctcctcgagc gtgtcgaaat gcagatggat ctggttgagc gtgtcgcccg cgctggtcca
     1561 gcccatcagc ggatcgggca ggcgcggcgt cgcctgctcg tagtcgagcc gccatttccg
     1621 cgtccctgcc cgccccgact gcatcgccgt cttgacaggt tgatagatgc gcgcttgtgc
     1681 catggctgct cttcggcctt ctttccgctt gcgctctcgg atggtcgggg cgagaggatt
     1741 cgaacctccg gcatccagct cccaaagctg gcgctctacc aggctgagct acgccccgag
     1801 agacccccga cggaacgctg gcggttctat ccgctcatgc ccgaaagcgc aagcgtcgcg
     1861 cggaaatggc cggcaccctc ttccctttcg gcagcgtctt ctgcaggatc gggcccttcg
     1921 ctccagagga cgcttcccat gaccaaacgc tcgatcgggc tcagcgccaa gctccatgac
     1981 tacattctga gcgtgtcgct gcgcgagccc gagatcctgc gccagctgcg ggaagaaacc
     2041 gcgaagctgc cgctggccgt catgcaggtg gcgccggacc agggccagtt catgcagctg
     2101 ctcgtgcgcc tcaccggcgc gcggagctat ctcgagatcg gcaccttcac cggctacagc
     2161 acgctggccg tggcgctggc gctgccgccc gacggccgca tcctcgcctg cgacatcaat
     2221 cccaagacca ccgaggtggc gcagcgctat tggaccgccg ccggcgtcgc ctccaagatc
     2281 gagctggtcc tggcgccggc gctcgagacc ctcgacaagc agctgaccca gggacaggcc
     2341 ggacgcttcg acctcgcttt catcgatgcc gacaaggaga attacgacgg ctattacgag
     2401 cgggcgctgc ggctggtccg gccgggcggg ctcatcctgc tcgacaacgt gctgtgggac
     2461 ggcgccgtcg ccgacccgaa gaagaacgac gccgacacca aggcgctgcg cgcggtgaac
     2521 aagaagatcc agggcgacga gcgggtggat gtgagcatgc tgccgttggc ggatggactc
     2581 actttggcgc gaaagcgcta ggcgaacgcg cgaagcgcgt tcgtccgcgg cggcgccagc
     2641 gattgctctt gcaatcgcgg aagccgcacc ccgacggcta gcctcctctg tgcgggctct
     2701 cgcggttgca agagcaaccg ctgccgcccg ccaaactaac gcgacgcttc gctatgcgaa
     2761 gcgtcgcgtt agttatgaaa tatcgggtta tggatgatgt cgccgacgcg gatgccgagc
     2821 tgcgccaccg tgtcgccttt gacctcgagc acggcgcgga ccgggacact ggattgaatc
     2881 acgtcctccg agagcggcac ggcatgctcg tggatgtcgg cgacgcgccc atccgcggtg
     2941 atgaacagca tgtccagcgg gatgagcgtg ttcttcatcc agaagcttgc cggctttgtc
     3001 gcgccgaaat cgaacagcat gccggcatcg ggcgcgagcg aggggcggaa catcagcccg
     3061 agctcctgct gctccggcgt cagggcgagc tcgacggcga aatggtgcgg cccggcaatc
     3121 gtgtcgatgg tgatcgtgga tttgtcgaag gacgcgacct catcggcgcg ggccgcggcg
     3181 gccggcgcca gcgacacgag gagcatgaga gcggcaatgc ggagacggag catggcggaa
     3241 tgtgcccaca gatagaacaa gttacgacgc ttctagcatc tcgatcgcag ggccggcaac
     3301 tcccgaaacc atcacttttc ggtcaatcgc ccgtaatcga atgtaaagga gcccccgata
     3361 ggctttcgcc ctttgccttg ggcggagcga tagggctcga tgacggattt gctacccacc
     3421 accgccgagc tgggcacccc tgcgccggcg cgcgggttca tgggcgcctt gcgcagcgga
     3481 agctggctcg accgtcgccg catcgtcggc tattgcgttg ccctgctgat cttcgaggtc
     3541 gtcgcctttt gcatcgcggt ggtagcgacc catggctggg tcgtgccgct gaacgagccg
     3601 accaccaccg atttcgccag cttctatgcc gcgggtgtcc tcgccaatgc cgggacgccc
     3661 gggctcgcct atgactcggt ccggcatttc gccgtcgagc atcaggtgac cggcggcgtc
     3721 aattatcagt tcttcttcta tccgccggtc tatctgctgg tctgtgccgg gctggcgcgg
     3781 ctaccttata tggtcgcctt catcctcttc gaggcggcga cggcggcgct ctatctcgtg
     3841 gtggcccgcg gcatcctcgg cgagaagaac ggcgcggcga gcctcgtcct cctctccttt
     3901 ccggcagtgt tctggaccat cggcctcggc cagaacgcgc tgctgacggc atctctcttc
     3961 ggcgcggcga cgcttctcgt ggaccgccgc ccggtgctgg cgggcctgct cttcggcgcg
     4021 ctctgctaca agccccatgt cggcctgctc atcccgctgg cgctggccgg cggcgggcgc
     4081 tggcgctgct tcgccgcggc ggccgcgagc gccagcctgc tcgtgctgct gtcgctggcg
     4141 ctgttcggcg tccagacctg gatcgatttc ctcgctgccg tcaccaacgc gcccgacacc
     4201 taccagtccg gccgcatctc gcttttcgcc atgatcagcc ccttcggcgc cgtgcggctg
     4261 atgggcggcg gcccggtgct cgcctatggc gcccagctcg ccgccgtcgc cgccatggcg
     4321 gggatcgtcg ccgtgatctg gcgcctgcgc ctcagcgagc cggtgagagc cgctattctc
     4381 gccgcggcga cgccgatcgc catgccggtc gcgctgatct acgacatgat gctcgccggg
     4441 atcgccatgg cctggctcgc ccgcgccgcc cgcgcgaccg gcttcctgcc ctgggagaag
     4501 atggctctgg cgggcttgtt cgccggcgcg ctcctgtcct tcaccttgag cggcccgctc
     4561 cacattccct tcgcgttgct atcgcttctc ggtcttgtcg cggtcatcgt cgcccgcgcc
     4621 cgctgggagc tgacccgcgc ccgcgatccc gcctgaagaa aaaggaagaa gtt
//
LOCUS       DASYNS010000286         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1159301, whole genome shotgun sequence.
ACCESSION   DASYNS010000286 DASYNS010000000
VERSION     DASYNS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159301"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..993)
                     /locus_tag="VGP50_08875"
     CDS             complement(67..993)
                     /locus_tag="VGP50_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027530.1"
                     /translation="MGPGRMVAPKVSLQGVLVDSRGKAWPDDSPDLAQRYGYGPPEAA
                     FVPYAIRERGCIHLVRQADSVRITLRAGKFSQRTLLGALLALETWPARILLSICDSAA
                     WSHEMFMSIGTFAEYAEDLAADKPYGTRQPWLASELGTEALGQPRLAALKPLFQVWQS
                     ARGRIPDDFRGTIESLGLGHRAILLRQMSESDRLVHEMVPTGVPYMKPCEWLTLIGRD
                     VENLPDQRYAAWAKRGYLEVLSNRAPRVDAVRATIRTIDGTMLRSRYYRLAIPWYCAG
                     DLFISVVSVRRELSISPRRNAEAISARTLARS"
     gene            complement(998..1426)
                     /locus_tag="VGP50_08880"
     CDS             complement(998..1426)
                     /locus_tag="VGP50_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV8027531.1"
                     /translation="MAQDKLRREPRVVARPRKSSKGRGTELDFLVGRRLREARLLTGI
                     TQGQLGQELGVSFQAVQKYESGENRLAVGRLAKAAKVLERPVSYFFTEEETASGSVVG
                     LTAQEAELIRSFRGIERPDLRESLLKLARQINELHAQLRE"
     gene            complement(1625..3043)
                     /locus_tag="VGP50_08885"
     CDS             complement(1625..3043)
                     /locus_tag="VGP50_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV8027532.1"
                     /translation="MTAQSHPLVLLVEDTEPLASLYIAYLMKEPVRTRHVATLEAARD
                     ALLTLRPAALLLDLELPDGNGLELIQTARSAGLDPAVIVITAHGSVNAAVEAMRAGAH
                     DFLVKPFNADRLLTTLRNALERRRLASIVETLGTEINRPSFEGFIGGDLAMHAIYRVI
                     ELAARSRSTVFITGESGTGKEVCAEAIHRRSPRKNGPFVALNCGAVPKELMESEIFGH
                     VKGAFTGATADRDGAALRAHGGTLFLDELCEMDPALQVKLLRFVQTGAVQPVGGDQTR
                     KVDARIICATNRDPLAEMEAGRFREDLYYRLHVLPIQMPALRERGGDVVLLARHFLAA
                     FSREEGRAFRGFAPDAEARLCASAWPGNVRQLQNVIRQVVVVQDGELVTAGMLPPEID
                     AERATESERAASPAAAATSPTHPHAAAQDEVLPLATVERQAIERAIALCGGNMTEAAA
                     RLGVNVSTIYRKRQNWGGKAAE"
     gene            3119..>3554
                     /locus_tag="VGP50_08890"
     CDS             3119..>3554
                     /locus_tag="VGP50_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV8027533.1"
                     /translation="MGMTALAPGDRDRLFAEIRGCTRCAAELPRGPRPVLRGLPSARL
                     LIISQAPGTRVHETGLSFNDRSGDRLRQWLGIDREAFYDENRVAILPMGFCYPGRHPQ
                     GGDLPPRPECAPLWHRRALAAWPGIALTLLVGSYAIDYYLAGR"
BASE COUNT          563 a         1215 c         1184 g          592 t
ORIGIN      
        1 ggtcgaggcc gaagaaggcg tggcgcttcg cctgggggcg cgcgatcttc cggcggtgct
       61 cagctatcag gaccgcgcca gcgttctcgc ggagatcgct tcggcgttgc ggcgcggcga
      121 gatcgagagc tctcgcctca ccgacacgac gctgatgaac aggtcgccgg cgcagtacca
      181 gggaatcgcc agccgatagt atcgcgagcg gagcattgtg ccgtcgatgg tgcggatcgt
      241 cgcgcgcacg gcatcgacgc ggggcgcgcg gttcgaaagc acctcgagat agcctcgctt
      301 cgcccaggcg gcatagcgct ggtccggcag gttctcgacg tcgcgcccga tgagagtcag
      361 ccactcgcag ggtttcatat agggcacacc cgtcggcacc atctcgtgca cgagacggtc
      421 gctctcggac atctgtcgca gcaggatcgc gcggtggccg agcccgagcg attcgatcgt
      481 gccgcggaaa tcgtccggta tgcggccgcg cgcggactgc cacacctgga agagcggctt
      541 cagcgctgcg agcctgggct ggcccagcgc ctcggtgccg agttcgctcg cgagccacgg
      601 ctggcgcgtc ccgtaaggct tgtcggcggc gagatcttcg gcgtattcgg cgaaggtgcc
      661 gatgctcatg aacatctcgt gcgaccaggc cgcgctgtcg cagatagaca gaagaatgcg
      721 cgccggccag gtctcgagcg caagcaatgc gcccagcagc gtgcgttgcg agaatttccc
      781 ggcacgcaac gtgatgcgca cgctgtccgc ttgccgcacc aggtgaatgc agccgcgctc
      841 gcggatcgca tagggcacga atgccgcctc gggcgggccg taaccatagc gttgcgcaag
      901 gtccggtgaa tcgtccggcc aagccttgcc gcgactgtcc accaggacgc cttgcaggct
      961 caccttcggc gcaaccatgc ggccgggacc catccgttta ttctcggagc tgggcgtgaa
     1021 gctcgttgat ctgacgcgcc agcttcaaca ggctctcgcg gaggtccggg cgctcgatgc
     1081 cgcggaagct gcggatgagc tctgcctcct gcgcggtcag gccgacgacg ctgcccgatg
     1141 ccgtctcctc ttccgtgaag aaataggaga ccggccgctc cagcaccttg gcggccttgg
     1201 ccagccgtcc cacggccaag cggttctcgc cggactcgta tttctgcacg gcctggaagg
     1261 acacgcccaa ctcctggccg agctggcctt gcgtgatccc cgtcaggaga cgcgcctcgc
     1321 gcaggcgtcg gccgacgagg aaatcgagct cggttccgcg tcctttgctg ctcttgcgcg
     1381 gtctcgcgac gacccgcggc tcccttctca acttgtcttg cgccatatcg ccctcggttc
     1441 ccttgattat ccacctataa cctagcaccg aacgctcctg ggcaacccta gataatggca
     1501 gttgccgctc tacacgggag tgtgcgcccg aatacttgct ccgagcacag tgttacattg
     1561 tcggccgatc gtcaacaaaa gcaaagtggg atggttcaca tcgcggacgg gtgccgcgca
     1621 ggggctattc ggcagccttt ccgccccaat tttgccgctt gcgatagatc gtcgatacgt
     1681 tgacgccgag ccgcgccgcc gcctcggtca tgttgccgcc gcagagggcg atagcccgct
     1741 cgatcgcctg ccgttcgacc gtcgcgagag ggagcacctc atcttgggcc gcggcatgcg
     1801 ggtgtgtggg cgatgtcgcc gcggccgcag ggctagccgc gcgctcgctt tccgttgcgc
     1861 gctcggcgtc gatttccggc ggcagcatcc cggccgtcac cagctcgccg tcctgaacga
     1921 ccacgacctg gcggatcacg ttctgcagct gccgcacgtt gccgggccag gccgaggcgc
     1981 agagtcgcgc ttccgcgtcg ggcgcgaagc cgcggaaggc gcggccttcc tcgcggctga
     2041 aggcggcgag gaaatgccgc gccagcagca cgacatcacc gccgcgctcg cgcagcgccg
     2101 gcatctgtat cggcagcaca tggaggcggt aatagagatc ctcgcggaag cgtcccgctt
     2161 ccatttcggc cagcggatcg cgattggtgg cgcagatgat gcgcgcatcg accttgcgcg
     2221 tctgatcgcc gccaaccggc tgcacggcac cggtctgcac gaaacgcagc agcttcactt
     2281 gcagcgccgg gtccatctcg cacagctcgt cgaggaagag cgtgccgcca tgggcgcgaa
     2341 gcgccgcgcc gtcgcggtcg gcggtggcgc cggtgaaggc gcccttcaca tggccgaaga
     2401 tctcgctctc catcagctct ttcggcacgg cgccgcaatt gagcgcgacg aaggggccgt
     2461 tcttgcgcgg gctgcggcgg tgaatcgctt cggcgcagac ctccttgccg gtgccgcttt
     2521 cgcccgtgat gaagacggtg gagcggctgc gcgcggcgag ctcgatgacg cggtagatgg
     2581 cgtgcatcgc aagatcgccg ccgatgaagc cctcgaagga aggacgattg atctcggtgc
     2641 cgagggtctc gacgatgctg gcgagacgcc gccgctcgag cgcattgcgc aacgtggtga
     2701 gcaggcgatc ggcattgaag ggcttcacca ggaaatcatg cgccccggcg cgcatcgcct
     2761 cgaccgcggc attgaccgag ccatgagcgg tgatgacgat caccgccggg tcgagcccgg
     2821 ccgaacgcgc cgtctggatg agttccagcc cgttcccatc ggggagctcg agatcgagca
     2881 gcagcgccgc cggccgcagc gtgaggagcg catcacgcgc cgcctccagc gtcgcgacat
     2941 ggcgcgtccg caccggctcc ttcatcaggt aagcgatgta gaggctggcg agcggctcgg
     3001 tatcctcgac caggaggacc aggggatgcg actgcgcggt cacgcgagcg acgctgccac
     3061 ggagccgtta acatccgaca aagcctcacg ccttgacggg tccgctagtc tcccgcccat
     3121 gggaatgacg gcgcttgcac ccggagaccg cgatcggctc tttgccgaga tccgcggctg
     3181 cacccgctgc gcagccgagc tgccgcgcgg gccgcggccg gtcctgcgcg gcctgccctc
     3241 ggcgcggctc ctcatcatca gccaggcccc cggcacgcgg gtgcatgaaa ccggcctctc
     3301 tttcaacgac cggagcggcg accggctacg gcaatggctg ggcatcgacc gcgaagcctt
     3361 ctacgacgag aaccgcgtcg ccatcctgcc gatgggcttc tgctatcccg gccgccatcc
     3421 ccaaggcggc gaccttccgc cgcggcctga atgcgcgccg ctctggcata ggcgcgccct
     3481 tgccgcgtgg ccggggatcg cgctgaccct gctggtcggt tcttatgcca tcgactatta
     3541 cctcgccggc cggg
//
LOCUS       DASYNS010000287         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1784501, whole genome shotgun sequence.
ACCESSION   DASYNS010000287 DASYNS010000000
VERSION     DASYNS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1784501"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VGP50_08895"
     CDS             complement(<1..831)
                     /locus_tag="VGP50_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8027534.1"
                     /translation="MKTSLESLPLVDRVAVPALVIGANGRVVAANASLAQVLGVDIAA
                     LRDSALADWLIDAAPLEAFLRGAPGTCGEFVFRGGDGAARRLALTRALADAGGDLFTV
                     FDLTPLAAAEQQLREEMRRYQDMTGATSDFFFELDATMTRLRILRRARDGRLELLEQQ
                     RKWPDEVIDLTYDLDAFAKVIRKMLAREPVRDYIHRQRDADGSERFLRSGGVPYYDAQ
                     GVFQGYRGVSVDVTAQVLAERAVRDSEARLRRSQQHLDQAQRIAATGSVERDLATGAE
                     E"
     gene            1017..2399
                     /locus_tag="VGP50_08900"
     CDS             1017..2399
                     /locus_tag="VGP50_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8027535.1"
                     /translation="MEAVRAEKVKAAELRLLAISPVTMVGATITLAFALIILWSAIDH
                     HLLFGWSAAVVTVLALRAGLWARFRRIRDDDAQVIRWAWPVTIVVTMSGILWGLFGIS
                     FYLVEDAEVRGAIMLILASMLASGTIFYSAYLPAHAGYVLGCALPIAAAAFWHGSLIS
                     TLFGCLTLGYITLISAGARGFNRGIVGAIRLQLENAALVDSLRSAKEAAEAANRTKSQ
                     FLAHMSHELRTPLNAVIGYSEILLEDADGDRGEEHFSDLKRINSAGRHLLSLVNDVLD
                     LSKIEAGRMELVMAPIDPDRFIQQVVETCRALVAEHGNKLVIERGSDLGSFIGDATKL
                     RQVVLNLVANAAKFTENGVITLSVARKRQADGDWLALAVRDTGIGISPENLEKLFTNY
                     TQADPLIGQKYGGTGLGLALSQRFCRLMGGEIVSESVIDRGSCFTIRIPVAMTGATPA
                     FDRRPVEHVG"
BASE COUNT          426 a          884 c          852 g          424 t
ORIGIN      
        1 ttcctcggcg cccgtggcga ggtcgcgctc gacgctgccg gtggcggcga tgcgctgcgc
       61 ctgatcgaga tgctgctggc tgcgccggag ccgtgcctcg ctgtcgcgca cggcgcgctc
      121 ggccagcacc tgcgccgtca cgtcgacgct gacgccgcga tagccctgga agacgccttg
      181 cgcatcgtaa taagggacgc cgcccgaacg caggaagcgc tcgctgccat cagcgtcgcg
      241 ctggcggtgg atgtagtcgc gcaccggctc gcgcgccagc atcttgcgga tgaccttggc
      301 gaaggcgtcg aggtcgtagg tgaggtcgat gacctcgtcc ggccacttgc gctgctgctc
      361 caggagctcg aggcggccgt cgcgggcgcg gcgcaggatc cgcagccgcg tcatggtggc
      421 gtcgagctcg aagaaaaagt cggaggtggc gccggtcatg tcctgatagc gccgcatctc
      481 ctcgcggagc tgctgttcgg cggcagcaag cggcgtcaga tcgaagacgg tgaagaggtc
      541 gccgccggca tcagcgagtg cgcgcgtcag cgcgagacgc cgcgcggcgc cgtcgccgcc
      601 gcgaaaaacg aactcgccgc acgtcccggg agcaccgcgc agaaacgcct cgagcggcgc
      661 cgcgtcgatg agccaatcgg cgagcgcgct gtcgcgcagc gcggcgatgt cgacgccaag
      721 cacctgcgcc agcgaggcat tggccgcgac gacgcgcccg ttggcgccga tcaccagcgc
      781 cgggacggcg acgcggtcga ccaacggcag ggactcgaga ctggtcttca aagcggctcc
      841 ggcagcccta gaacgcaacc ttcgatggta cgaccctatc cttgaccaac cgttagtttt
      901 ttatcattcg ggcgccggcc ccgagggacc gacgtcttaa gccgcatggt catggtgaac
      961 cgccgcacag gaaaaattaa gcctcccttc ctaggctcta ccccaaggag ggttccgtgg
     1021 aggcagtgcg ggcggagaag gttaaggcgg cagagctcag gctcctcgcc atctcgccgg
     1081 tgacgatggt cggcgcgacc atcacccttg cctttgcttt gatcatcctg tggtcggcga
     1141 tcgaccacca cctgcttttc ggctggtcgg cggccgtggt gaccgtcctc gccctccgcg
     1201 cggggctgtg ggcgcgcttt cggcgcatcc gcgacgacga cgcgcaagtc atccgctggg
     1261 cgtggcccgt cacgatcgtc gtgaccatgt cgggtatcct ctgggggctt ttcggcatca
     1321 gcttctatct ggtggaagac gccgaggtca gaggcgccat catgctcatc ctggcgagca
     1381 tgctggcgtc gggcacgatc ttctactcgg cctatctgcc tgcccatgcc ggctatgtgc
     1441 tgggctgcgc actgccgatc gccgcggccg ccttctggca tggcagcttg atatcgacgc
     1501 tcttcggctg cctcaccctc ggctatatca cgctgatctc cgccggcgcg cgcggcttca
     1561 atcgcggcat cgtcggcgcg atccgcctcc agctcgagaa cgcggcgctg gtcgacagcc
     1621 tgcgcagcgc caaagaggcg gccgaggcgg cgaaccgcac caagagccag ttcctcgccc
     1681 atatgagcca tgagctgcgc acgccgttga acgctgtgat cggctacagc gagatcctgc
     1741 tcgaagatgc cgacggcgac cgcggcgagg agcatttctc ggatctgaag cgcatcaaca
     1801 gcgccggccg gcacctgctg tcgctggtca acgacgtgct cgacctgtcg aagatcgagg
     1861 cgggacgcat ggagctggtg atggcgccga tcgacccgga ccgcttcatc caacaggtcg
     1921 tcgagacctg ccgcgcgctc gtcgccgagc atggcaacaa gctcgtgatc gagcgcggca
     1981 gcgatctcgg ctccttcatc ggcgacgcca ccaagctgcg ccaggtcgtg ctgaacctcg
     2041 tggccaacgc cgcgaaattc accgagaacg gcgtcatcac gctgagcgtc gcgcggaagc
     2101 gccaggccga cggcgactgg ctcgccctcg cggtgcgcga caccggcatc ggcatcagcc
     2161 ccgagaacct cgagaaactt ttcaccaact acacccaggc cgacccgctg atcggccaga
     2221 aatatggcgg gaccggcctc ggcctcgcct tgagccagcg gttctgccga ctcatgggcg
     2281 gcgagatcgt ctccgagagc gtcatcgacc gcggctcctg cttcaccatc cgcattcccg
     2341 tcgccatgac cggtgccacg ccggcattcg accgccgtcc cgtcgagcac gtcggatagc
     2401 cggctagcga agtcgtccgt tatccgttat cagtcgtcgg tcgggcgcaa cggacatctg
     2461 acaaccgatg gctggcttat ccatctccga agcgaccgcg gcgctgacgg cgccgggcgc
     2521 tcccttcgca atggcagaga aagagatcgg cggcatcaag ctgcgggtgt ggcggaacgc
     2581 gccgcc
//
LOCUS       DASYNS010000288         4659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3657242, whole genome shotgun sequence.
ACCESSION   DASYNS010000288 DASYNS010000000
VERSION     DASYNS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3657242"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VGP50_08905"
     CDS             <1..1037
                     /locus_tag="VGP50_08905"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEV8027536.1"
                     /translation="REARFIDTSGGGADGGVHDLFVITGRTDATNQPNANTGTNTTCT
                     VPQTNFPQQIASNNIIFRIPISVFGDGLVENIGELTLKSTPSSGSASASASSLGITVG
                     KFNHSPNDGTITRFGWKAQNKSLLIFAGEAYNVEQGVTNELFTNERAEQFQDGFESET
                     AEAIKDCFFNATPEDATAFENTRNSNSPASDFSSDIVNFAGFMRLLNQPTPAASTTAT
                     EEGETAFLNIGCGICHTPTFTTDASIFTAQSEVAVSPFSDFALHAMGTVLQDQVKQGQ
                     ANGEEFRSAPLFGVGQRAFLLHDGRTQNLVTAIEDHASSGSEASQVITNFNNLTATEQ
                     QNIVTFLRSL"
     gene            complement(1106..1999)
                     /locus_tag="VGP50_08910"
     CDS             complement(1106..1999)
                     /locus_tag="VGP50_08910"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8027537.1"
                     /translation="MTTVGVIGVGIMGSAIAGNLLGPDMTVIGFDSAADRRAALAAMG
                     GQAAASAAAVAGAAEIVLTSLPSSAALEETVAAILTRPPGRELVVAELSTLPIETKLR
                     CRDALAGAGIAMLDCPLSGTGAQALNRDLVVYASGDRAAFDRSAPVFARFSRSSHYLG
                     AFGNGSKMKFVANLLVAIHNVASAEAMVLGMKAGLDPADLVKVIADGAGTSRVFELRA
                     PMMARADYQPPSMKIGIWQKDMAVIGDFARNLGVATPLFAASEPIYNAAVGLGLGGDD
                     TAAVCAVLERMAGIARGSTSR"
     gene            complement(1996..2595)
                     /locus_tag="VGP50_08915"
     CDS             complement(1996..2595)
                     /locus_tag="VGP50_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8027538.1"
                     /translation="MTEAVRFFAEFGFNGQTRELAQRLGITQPLLYRYFPTKQDLIER
                     VFDELFLKRIDPQWTTLITDRSRPLGERLVDFYQRYARITYTYEWIRIYMFSALRGND
                     LNRRYIRIVEDRILRPICAEIRHYCALPIDESTPISEAELEHVWVMHGGLFYYAVRKY
                     IYHSRVGRDFNRTIQLTVDAMLAGVKAIGPAAGPQVRKP"
     gene            complement(2673..4421)
                     /locus_tag="VGP50_08920"
     CDS             complement(2673..4421)
                     /locus_tag="VGP50_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEV8027539.1"
                     /translation="MTPRGSEIRDGMRIDWDVPIPMDDGVVLRADVFRPVGDGRYPVI
                     LSHGPYGKGLAFQDGYPSAWERMASAHPDVTASSSNKYQNWEAVDPEKWVPDGYACVR
                     VDSRGTGRSPGRIDPFSPRETRDFHDCIEWAGVQPWSNGKVGLNGISYYAINQWHVAA
                     LAPPHLAAICVWEGAADWYRDMTHHGGILCTFWANWYDMQVKTVQHGLGERGPRSRAT
                     GDLVCGPETLTEDELASHRCDLAAEIRAHPLDDAYHRARSAEWSKVRVPLLSAANWGG
                     QGLHPRGNFEGFLRAASRYKWLEVHGIEHWTEFYTDYGVALQKRFFDHYLRNADNGWE
                     RQPRIQLQIRHIDRFVERHESEWPLARTRWTPFYLDPTGPGLVDAPRTEAQRITFDAQ
                     GDGVTFFSSPLAAATEITGPAAARLFVSSSTSDADLFLILRVFAPDGEEIVFQGALDP
                     HTPIGQGWLRASHRKLDAALSTLYRPYHCHDEKQPLVPGEPVTLDIEIWPTSIVVPAG
                     YRLGLTVRGRDYEHKGPPARLSNMKNDFRGCGPFLHDDPRDRPPEVFAGRTTLHMGPG
                     SPAYLLLPIIPEGGRR"
     gene            complement(4429..>4659)
                     /locus_tag="VGP50_08925"
     CDS             complement(4429..>4659)
                     /locus_tag="VGP50_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027540.1"
                     /translation="SAVTLDYVLDALVICGTVNSVVDRLLAFREQVGDFGTLLYAGHD
                     WVEPRLARRSMELMANEVMPRVNAAIKKTAAE"
BASE COUNT          851 a         1513 c         1546 g          749 t
ORIGIN      
        1 ttcgcgaggc gcgcttcatc gacacctcgg gcggcggcgc cgatggtggc gtgcacgacc
       61 tctttgtcat caccggccgc acggatgcca ccaatcagcc aaacgccaac acgggcacca
      121 acaccacctg cactgttccg caaaccaact tcccgcagca gattgccagc aacaacatca
      181 tcttccgcat cccgatcagc gtcttcggcg acgggctcgt ggaaaacatc ggcgagctga
      241 ccctgaagag cacgccctcc tcgggcagcg ccagcgcgtc ggccagcagt ctcggcatca
      301 ccgtgggcaa gttcaaccac agccccaatg acggcacgat cacccgcttc ggctggaagg
      361 cgcagaacaa gtcgctgctg atattcgccg gcgaggccta taacgtcgag cagggcgtca
      421 ccaacgagct cttcaccaac gagcgcgcgg agcagttcca ggacggcttc gagagcgaga
      481 cggccgaggc catcaaggac tgtttcttca atgcgacgcc cgaggacgcg accgccttcg
      541 aaaacacccg caacagcaac agcccggcct ccgacttctc gtcggacatc gtcaacttcg
      601 ccggcttcat gcgcctcttg aaccagccga cgccggcagc gtccacaacc gcgaccgaag
      661 aaggcgagac cgccttcctc aacatcggct gcggcatctg ccatacgccg accttcacga
      721 ccgacgcctc gatcttcacg gctcagagtg aagtcgcggt cagtccgttt agcgacttcg
      781 ccctccatgc aatgggcacc gtgctccagg accaagtgaa gcaagggcag gccaacggcg
      841 aagagttccg ctcagcgccg ctgttcggcg tggggcagcg cgcgttcctc ctccatgacg
      901 gccgcaccca gaatctggtg acggcgatcg aggatcatgc gagcagcggc tcggaagcca
      961 gtcaggtgat caccaacttc aacaatctca cggcgacgga gcagcagaac atcgtcacct
     1021 tcctccgctc gctctaagcc gagatcgatt tcctggagca gcggcaggcc gggccggccg
     1081 ctgctccttc ttcgtgacga taccgctagc gcgacgtcga gccgcgggcg atgccggcca
     1141 tccgctcgag aacggcgcag accgccgccg tgtcgtcgcc acccagtccc aggccgaccg
     1201 cggcgttata gatgggctcg cttgccgcaa agagcggcgt cgcgacccca agattccgcg
     1261 cgaaatcgcc gatgaccgcc atgtccttct gccagatgcc gatcttcatc gagggcggct
     1321 ggtagtcggc acgcgccatc atcggcgcgc gcagctcgaa cacgcgcgac gtgccggcgc
     1381 cgtcggcgat cactttgacg agatccgcgg ggtcgagccc ggccttcatg cccagcacca
     1441 tggcttcggc gcttgcgacg ttgtggatgg cgaccagcag attggcgacg aacttcatct
     1501 tgctgccgtt gccgaaggcg ccgagataat ggctcgagcg cgaaaagcgg gcgaagacgg
     1561 gcgcggaacg atcgaaggcg gcgcgatcgc ccgacgcata gacgaccaga tcgcgattga
     1621 gcgcctgtgc gccggtgccg ctgagcgggc aatcgagcat cgcgatgccg gcgccggcaa
     1681 gggcgtcgcg acagcgaagc ttcgtctcga tgggcaaggt gctgagctcg gcgacgacga
     1741 gctcacgccc cggcggacgc gtgagaatgg cggccaccgt ctcctcgagg gcggcgctgc
     1801 tgggcagcga tgtgaggacg atctccgccg cgccggcgac cgccgcggcc gaagccgcgg
     1861 cctggccgcc catcgccgcg agcgccgccc ggcggtcggc ggcactatcg aagccgatca
     1921 ccgtcatgtc cgggccaagc aaattgccgg cgatcgccga gcccatgatg cccaccccga
     1981 tcacgccgac cgtggtcatg gctttctcac ctgcggcccg gccgccgggc cgatcgcttt
     2041 gacgccggcg agcatggcgt cgaccgtgag ctgaatagtc cggttgaaat cgcggccgac
     2101 gcggctgtgg tagatgtatt tgcggacggc gtaataaaag agtccgccat gcatgaccca
     2161 gacatgctcg agctcggcct cgctgatcgg cgtggactcg tcgatgggga gcgcacagta
     2221 gtggcggatc tcggcgcaga tgggcctgag tatccggtcc tcgacgatcc tgatatagcg
     2281 ccggttgagg tcgttgccgc ggagcgcgga gaacatgtag atgcggatcc attcataggt
     2341 ataggttatc cgcgcatagc gctgatagaa gtcgacgaga cgctcgccga gcggccggga
     2401 gcggtcggtg atgagggtgg tccattgcgg atcgattcgc ttcaggaaga gctcgtcgaa
     2461 gacccgctcg atcaggtcct gcttcgtggg aaaatagcgg tagagcagcg gctgcgttat
     2521 gccgagccgc tgcgccagct cgcgcgtctg cccgttgaag ccgaactcgg caaagaagcg
     2581 caccgcctcg gtcacgatct gctgctcgcg gaccgctgcc gcaagccgct tgttggactt
     2641 gagcggtcgc agcggtcgcc gtttggggga agctatcgcc ggcctccctc ggggatgatc
     2701 ggcagaagca gataggcggg agagccgggt cccatatgaa gggtggtccg gccggcgaag
     2761 acctccggcg ggcggtcgcg cggatcgtcg tgcaggaacg gtccgcaacc cctgaagtcg
     2821 ttcttcatat tggagagtcg cgcgggcgga cccttgtgct cgtagtcgcg cccgcgcacg
     2881 gtgagaccga gacgataacc cgccggcacc acgatcgacg ttggccagat ctcgatatcg
     2941 agtgtcaccg gctcccccgg taccagcggc tgcttctcgt cgtgacagtg ataaggacga
     3001 taaagcgtgc tgagcgccgc gtcgagcttg cgatgcgacg cgcgaagcca accttggccg
     3061 atgggcgtgt gcggatcgag agccccctgg aagacgattt cttcgccatc gggcgcgaag
     3121 acgcgcagga tgaggaacag atcggcatcg ctggtcgagg acgacacgaa gagccgtgcc
     3181 gccgcggggc ctgtgatctc ggtcgccgcg gcgagcgggc tggagaaaaa ggtgaccccg
     3241 tcgccctgag cgtcgaacgt gatgcgttgc gcttcggtgc gtggggcatc gacgaggccc
     3301 ggcccagtgg gatcgagata gaacggcgtc caacgggtgc gcgcgagcgg ccattcgctc
     3361 tcgtgacgct cgacgaagcg gtcgatgtgg cgaatctgga gctggatgcg cggctggcgc
     3421 tcccagccat tgtcggcgtt cctgagatag tggtcgaaga aacgcttctg cagcgcgacg
     3481 ccatagtcgg tgtagaactc ggtccaatgc tcgatgccat gcacctcgag ccatttatac
     3541 cgcgacgccg cgcgaaggaa cccctcgaaa ttgccgcgcg ggtgcagacc ctgtccgccc
     3601 cagttggcag ccgagagcag cggcacccgg accttggacc attcggccga gcgagcccgg
     3661 tggtaggcgt cgtcgagcgg atgggcgcgg atctcggcag cgagatcgca acgatggctg
     3721 gcgagctcgt cctcggtcag ggtctccggc ccgcagacga ggtcgccggt ggcgcggctc
     3781 cgggggccgc gttcacccaa accatgctgc acggtcttca cctgcatgtc gtaccaattg
     3841 gcccagaagg tgcagaggat gccgccatgg tgggtcatgt cgcgatacca gtccgcggca
     3901 ccttcccaga cgcagatcgc ggcgagatgc ggcggggcga gcgccgccac gtgccactga
     3961 ttgatggcgt agtaggaaat gccgttcagt ccgaccttgc cattgctcca gggctgcacg
     4021 ccggcccatt cgatgcagtc gtgaaagtcc ctggtctcgc gcggcgagaa ggggtcgatg
     4081 cggccgggcg agcgcccggt gccgcgcgag tcgacgcgga cgcaggcgta gccgtcaggc
     4141 acccatttct cggggtccac ggcctcccag ttctgatact tgttcgacga gctggcggtg
     4201 acgtcgggat gcgcgctcgc catccgctcc caggccgagg gataaccgtc ctgaaaggcg
     4261 agacccttgc catagggtcc atgactcagg atcacgggat agcggccatc gccgacgggg
     4321 cggaacacgt cggcgcggag gacgacgccg tcatccatgg ggatcggcac gtcccaatcg
     4381 atgcgcatgc cgtcgcgtat ttcgctcccg cgaggggtca tcgcatgcct attcggcggc
     4441 ggtcttcttg atcgcggcgt tgacgcgcgg catgacctcg ttcgccatca gctccatcga
     4501 gcgccgggcg agtcgcggct cgacccaatc atgtccggcg tagagcagcg tgccgaaatc
     4561 gccgacctgc tcgcgaaagg ccagaagccg gtcgaccacg ctgttgaccg tgccgcagat
     4621 gacgagcgca tcgagcacgt agtccagggt gacggcgga
//
LOCUS       DASYNS010000289         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2409303, whole genome shotgun sequence.
ACCESSION   DASYNS010000289 DASYNS010000000
VERSION     DASYNS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2409303"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="VGP50_08930"
     CDS             <1..1624
                     /locus_tag="VGP50_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV8027541.1"
                     /translation="LGGAAPIIATAHHDNHAWFSFAVSPFAKSERPVMVAAIDGFGDR
                     GAITLYVCDGGVMRELYCNDSMFDSLGVFYAVISSTQGGWTLLSSEGRYMGAAAWGNY
                     DRKTNPFYAPLRAILQLAPDGKIFVNRSLANWPRNMAKPYTKDLIRILGEPIALKDMW
                     NPDAMLRVEDIRHKEDTQERLDKAAATQMVFEDALIHIIDHLIRTTGSDQLVLTGGTA
                     LNAVASMRLLEHFDEAYYQRQFGRRARLHLWVPPTPNDAGVAMGAAFMGAYLAGAGPG
                     EPLAHAFYCGSAPSDAAIRDAFAAAPDVSWAPIGSVTSAAGREAIADLMAYMTAQDGI
                     IALYQGAAETGPRALGHRSILANPRNPRTREFLNARVKYREAIRPLAPMMTREAALKW
                     FELSDGAADADYNGYNYMVLTAQAKPGAAARIPAVIHADGTGRLQIVREATDPLTHAY
                     LKALGRRNGEEVAVNTSFNVAGPIAQTPEQAIETLRRSKGMDAVVMIAEEGAAYAMWR
                     KGQDGNRFEHWHAEWKDDGRRTTDDGGRIGIR"
     gene            complement(1721..>2164)
                     /locus_tag="VGP50_08935"
     CDS             complement(1721..>2164)
                     /locus_tag="VGP50_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEV8027542.1"
                     /translation="AMLIVFIALVYLANAMLGLLPDLFGAPVTLQRTLGWVMAPICWL
                     MGIPWAQAVTAGGLMGIKTILNELVAYLQLAALPADALDPRSRLIMLYAMCGFANFGS
                     LGIMIAGLSTMCPEKRDEVVSLGLKSIVSGTLTTCLLGAIVGVLS"
BASE COUNT          403 a          724 c          700 g          337 t
ORIGIN      
        1 gctcggcggc gcggcgccca tcatcgccac ggcgcatcac gacaatcacg cctggttctc
       61 gtttgcggtg tcgccgtttg ccaagagcga acgcccggtg atggtcgcgg ccatcgacgg
      121 ctttggcgat cgcggcgcga ttacgcttta tgtctgcgac ggcggcgtga tgcgggaact
      181 ctactgcaac gacagcatgt tcgactcgct cggcgtcttc tatgcggtca tctcgtcgac
      241 ccaaggcggc tggacgctgc tctccagcga aggccgctac atgggcgccg cggcttgggg
      301 caattacgac cgcaagacca atccatttta tgcgccgctg cgcgcgatcc tgcagctggc
      361 gcccgacggc aaaatttttg tcaatcgctc gctggcgaat tggccgcgca atatggcgaa
      421 gccctacacc aaagacttga tccgcatcct cggcgagccg attgcgctca aggacatgtg
      481 gaatcccgat gcgatgctcc gcgtcgagga catccgccac aaggaggaca cccaggagcg
      541 gctcgacaag gccgccgcca cccaaatggt gttcgaggat gcgctgatcc acatcatcga
      601 ccacctgatc cgcacgaccg gcagcgacca attggtgctg accggcggca ccgcgctcaa
      661 cgccgtcgcc agcatgcggc tcctcgagca tttcgacgag gcctattacc agcgccagtt
      721 cggccgccgc gcgcgcttgc atctgtgggt gccgccgacg ccgaacgacg ccggagtggc
      781 catgggcgca gcctttatgg gggcctacct cgccggcgct ggcccgggcg agccgctcgc
      841 gcacgccttc tattgcggct cggcgccgag cgacgccgcg atccgcgacg cgttcgccgc
      901 cgcgcccgac gtgtcgtggg ccccgatcgg tagtgtgacc agcgccgcgg gccgcgaggc
      961 gatcgccgat ctcatggcgt atatgacggc gcaggacggc atcatcgcgc tctaccaggg
     1021 cgccgccgag accggaccgc gcgcgctcgg gcaccgctcg atcctggcca atccgcgcaa
     1081 tccgcgcacc cgcgagtttc tcaacgcgcg cgtaaaatac cgcgaagcga tccgcccgct
     1141 ggcgccgatg atgacgcgtg aggcggcgtt gaagtggttc gagctgtcgg acggcgccgc
     1201 cgacgccgac tacaacggct acaactacat ggtgctgacc gcgcaggcga agcccggcgc
     1261 ggcggcgcgc attcccgccg tgatccacgc cgacggcact ggccgcctgc agatcgtgcg
     1321 cgaagcgacc gatccgctga cgcatgctta tttgaaagcg ctcggccgcc ggaacggcga
     1381 agaggtcgcg gtcaacacct cgttcaacgt cgcgggcccg atcgcgcaga cgcccgagca
     1441 ggccatcgaa acgctgcggc gctcgaaggg catggatgcg gtggtgatga tcgcggagga
     1501 gggcgccgct tacgcgatgt ggcgcaaagg gcaggacggg aatcgcttcg agcactggca
     1561 cgcggagtgg aaggacgacg gacgacggac gacggatgac ggaggacgga tcgggatcag
     1621 atgacggacg acggacgaca gaggacagat cgcggcattg acgcttgagc cttaatccgt
     1681 tctccgtttt ctgtcctccg tcgtctgtcc tccgtcgtcc tcaactcagg actccgacaa
     1741 tggctccgag caaacacgtc gtcagcgtcc ccgaaacgat cgacttcaag cccagcgaca
     1801 ccacctcgtc gcgcttttcc gggcacatgg tggagagccc cgcgatcatg atgccgagcg
     1861 agccgaaatt ggcgaagccg cacatggcgt agagcatgat caggcgcgaa cggggatcga
     1921 gcgcatcggc cggcagcgcc gcgagctgca gataggcgac gagctcgttc aagatcgttt
     1981 tgatccccat cagcccgccc gcggtcaccg cttgcgccca cggaatgccc atcagccagc
     2041 agatcggcgc catcacccag ccgagcgtgc gctgcaaggt gactggcgcg ccgaacaaat
     2101 ctggcagaag cccgagcatg gcgttggcga gataaaccag cgcgatgaac acgatcagca
     2161 tggc
//
LOCUS       DASYNS010000290         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5528103, whole genome shotgun sequence.
ACCESSION   DASYNS010000290 DASYNS010000000
VERSION     DASYNS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5528103"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VGP50_08940"
     CDS             complement(<1..1430)
                     /locus_tag="VGP50_08940"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEV8027543.1"
                     /translation="MLLDTQRQARAAELIDSAAIVADLEALAATHAGRERELRAAVVP
                     RLRSALTQARARADELLLKDRHGRRCAERLCQTQDEIIRILFEFATGVLYPAQVRSQA
                     ERMTVIATGGYGRGLLAPGSDIDLLFVLPYKQTAWGESVAEALLYCLWDMGLKVGHAT
                     RSVNECIRQAKADMTIRTALLESRYLLGDATLYAELTARFDKEVVQGTAPEFVAAKLA
                     EREERHRRAGQSRYLVEPNVKDGKGGLRDLHTLFWIAKYVYRVREPEELIERGVFDRD
                     EYRLFCRCEDFLWSVRCNMHFLAGRAEERLSFDIQREIAVRLGYTEHPGLRDVERFMK
                     HYFLVAKDVGDLTAILCAGLEERQAKPAPVLSRVIARLRPARRQQRLTETDDFVIDNN
                     RINIAGPDVFKRDPVNLIRLFHLAQKHNLAIRPEAMRAATRSLTLIDLKLRENKEANR
                     LFLEILTSNNEAETVLRRMNEAGVLG"
BASE COUNT          265 a          523 c          570 g          280 t
ORIGIN      
        1 ttgccgagca cgccggcctc gttcatccgc cggagcacgg tttccgcctc gttgttcgag
       61 gtcagaatct cgaggaacag gcggttcgct tccttgttct cgcgcagctt cagatcgatc
      121 agggtgagcg agcgggtcgc cgcgcgcatg gcttcgggac ggatcgcgag attgtgcttc
      181 tgcgccaggt ggaacagccg gatcaagttg accgggtcgc gcttgaagac atcgggcccg
      241 gcgatgttga tgcggttgtt gtcgatgacg aaatcgtcgg tctcggtcag ccgctgctgg
      301 cggcgcgccg ggcgcagccg ggcgatgacc cggctcagca ccggcgccgg tttcgcctgg
      361 cgttcctcca ggccggcgca gagaatggcg gtgagatcgc cgacgtcctt ggcgacgagg
      421 aagtagtgct tcatgaagcg ctcgacgtcg cgcagacccg gatgctcggt gtagccgagc
      481 cgcaccgcga tctcgcgctg gatgtcgaac gacaggcgct cctcggcgcg gccggcgagg
      541 aaatgcatgt tgcagcggac cgaccagagg aaatcctcgc agcggcagaa caaccggtac
      601 tcgtcgcggt cgaacacgcc gcgctcgatc agctcctcgg gctcgcgcac gcggtagacg
      661 tatttggcga tccagaacag cgtgtgcagg tcgcgcagcc cgcccttgcc gtccttgacg
      721 ttgggctcga cgagatagcg cgactggccg gcgcggcggt gccgctcttc gcgctcggcg
      781 agcttggcgg cgacgaattc cggcgccgtc ccctgcacca cctccttgtc gaagcgcgcc
      841 gtcagctcgg cgtagagcgt ggcgtcgccg agcaggtagc gcgattccag aagcgccgtg
      901 cggatggtca tgtcggcttt ggcctggcgg atgcattcgt tgaccgaacg cgtcgcgtga
      961 ccgaccttga ggcccatgtc ccacaggcag tagagcaagg cctcggccac cgactcgccc
     1021 caggcggtct gcttgtacgg cagcacgaac aggagatcga tgtcggagcc cggcgccagc
     1081 agcccgcggc catagccgcc ggttgcgatc accgtcatgc gttcggcctg cgaacggact
     1141 tgcgccggat agagcacgcc cgtggcgaac tcgaagagga tgcggatgat ctcgtcctgg
     1201 gtctggcaca accgctcggc gcagcggcgg ccgtgacggt ccttgagcaa cagctcgtcg
     1261 gcgcgggcgc gcgcctgggt cagcgccgat ctgagacgcg gcacgacggc ggcgcgcaat
     1321 tcccgctcgc gcccggcgtg ggtcgcggcg agcgcttcga gatcggcgac gatcgcagcg
     1381 ctgtcgatga gttcggcggc gcgcgcctgg cgttgggtgt ccagcagcat ctcgtgtagc
     1441 ctggagacgg tcctatgcta ctattcttaa tacctccatt cgaaaagcgg caaccgccac
     1501 agggaaggat cgtccgatga aaagctatga tgtctgcgaa tgtggcgcgc cgctgcgact
     1561 gatggaacgg ccgacgccga agccgaccgg cgccgaggtc ctgctcaagg tcattgccgc
     1621 cggcgtctgc catagcga
//
LOCUS       DASYNS010000291         3702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2720373, whole genome shotgun sequence.
ACCESSION   DASYNS010000291 DASYNS010000000
VERSION     DASYNS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2720373"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VGP50_08945"
     CDS             complement(<1..1322)
                     /locus_tag="VGP50_08945"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8027544.1"
                     /translation="MSDDQKSVGAATTVELADILFSSSAPILGNWLITLIGGAALLYE
                     RYSPFILLWTIANSVVCVVRLRQWRSYRRQRQSMTDPQAWMRRNARLVVATGILWGML
                     GLVTVSGTDMMNSVMVPLMIFSISASIASTHSCYVPAVRAFIFPAYFLTGLGEIIGGD
                     APHLVIALCTLIYLANLELVARRGHRVVVQTLLSRFAKERSQEQLARAQRVAATGSTE
                     YDLATGVEECSEEMYHLLGIAGPRSFSLTEEENILAAIHPQDRERVRAMRVVQRAGHR
                     TRPGEFRVVRPDGEVKTLYAETDIQRDSAGRPLRVLTIVKDVTALRAAEQRQKEMQQQ
                     LLQAQKLEAMGTLAGGVAHELNNTLVPVLALAKLTIKRLPEGSREQNNLITILQAGEK
                     ARDLVARVVAFSRKEMPTRVEVDLATLTRDTLSLMRHSLPATIQLAER"
     gene            1659..3173
                     /locus_tag="VGP50_08950"
     CDS             1659..3173
                     /locus_tag="VGP50_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEV8027545.1"
                     /translation="MTVERQAGEGSAFSHREILHILSGVLLGMMLAALDQSIVATALP
                     AIASDLHGLADLSWIVAGYLLTSTAATPIYGKLSDLYGRRALLQFAIVVFLLASLLCA
                     AAQNIAELVAARALQGVGGGGLISMAQAVIADVISPRERGRYQAYMSGVWATASVGGP
                     VLGGFFVDHLSWRWVFWINLPLGIAAFVLCRRALARLATPRSHRRIDYLGAVLLTLAV
                     ADLLLLVTWGGSTLPWTSPQFLFLMMAAAPLVFAFIVQELRAVDPILPPRLFADPLIG
                     CANLASFIVSMAMFGGVLLLPVFLQLVTGVGAGHSGVLLIPLMGGSVAGAFTAGQLMR
                     RTGHYKRFALIGLVMSTLAFALLATMDEATRPAVAMGYMALLGIGVGSSMPVMLVAVQ
                     NAAEPRDIGVATASVAFCRSLGGAFGAAILWSVLLGALGHRLGGAALAGNLLEGADAT
                     ALTAAQRAVMVPALVPSFHLVFAIAALIAAASVVATLFLRELPLRTTPRGSAQR"
     gene            complement(3501..>3702)
                     /locus_tag="VGP50_08955"
     CDS             complement(3501..>3702)
                     /locus_tag="VGP50_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027546.1"
                     /translation="RDYLRIEMFNDHPLTTHGLVLKVLGQARENVVHGVAERPPVPAS
                     PPVGAWWRGSLKPWNQLRRVFG"
BASE COUNT          564 a         1230 c         1263 g          645 t
ORIGIN      
        1 atccgctcgg cgagctgaat ggtggcgggc aggctgtggc gcatcaagct cagcgtgtcg
       61 cgggtgaggg tggcgaggtc gacctcgaca cgggtcggca tctccttgcg cgagaaggca
      121 acgacgcggg cgacgagatc gcgggccttc tcgcccgcct ggagaatggt gatgagattg
      181 ttctgctcgc ggctgccctc gggcaggcgc ttgatcgtca gcttggcgag ggccagcact
      241 ggcacgagcg tgttgttgag ctcatgggcg acgccgccgg ccagcgtgcc catcgcctcg
      301 agcttctgcg cctgaagcag ctgctgctgc atctcctttt gccgctgctc ggcggcgcgg
      361 agcgcggtca cgtccttgac gatggtcagc acgcggagcg gccgtccggc gctgtcgcgc
      421 tggatgtcgg tttccgcgta gagcgtcttc acctccccgt cgggacggac gacgcggaac
      481 tccccggggc gcgtccgatg ccccgcgcgt tggacgacgc gcatggcgcg cacgcgctcg
      541 cggtcctgcg gatggatggc ggcgagaatg ttctcctcct ccgtcagaga aaaggatcgc
      601 gggccggcga tgcccagcag gtgatacatt tcctccgagc attcttcgac gccggtggcg
      661 aggtcgtact cggtgctccc cgtcgccgcg acccgctgag cgcgcgccaa ctgctcctgg
      721 ctgcgctcct tcgcgaaccg ggacagcaac gtctggacga cgacgcgatg accgcgacgc
      781 gccaccagct ccaggttggc gagatagatg agcgtgcaca gcgcgatcac gagatggggc
      841 gcgtcgccgc cgattatctc gcccagaccc gtaaggaagt aggccggaaa gatgaaggcc
      901 cggacggctg gcacatagca gctatgcgtc gaggcgatgc tcgccgagat cgagaagatc
      961 atcagcggca ccatcacgct gttcatcatg tcggtgcccg acacggtcac caaacccagc
     1021 atgccccaga gaatgccggt ggccacgacc agccgggcgt tgcggcgcat ccaggcctga
     1081 gggtcggtca tgctctgccg ctgccgccga taagagcgcc attggcgcag acgcacgacg
     1141 cagacgacgc tgttggcgat ggtccagagc aggatgaagg gcgagtagcg ctcgtaaaga
     1201 agcgctgcgc cgccgatgag cgtgatcagc caattcccca ggatcggcgc gcttgacgaa
     1261 aacagaatat ccgcaagctc gacggtggtg gcggcgccta ccgatttctg atcgtcgctc
     1321 atcacctcgt cccgccggac actcggccat gccagccctg cggcagacgt tccgccagac
     1381 aacggggcac cgaacaagcg atgtccgcgg gtcgggagtc ctggaccatg gacgcaaacc
     1441 tatgccgaag ggatttataa acctttaccc ccgtcgcatc cgggcgagcg cgctgccgaa
     1501 ctcgctatgc atggcaagtc cccgttcgat gcatgagggg gaaacaaatt gacgaaaact
     1561 ccagccgatc atcggcggaa gccacgccct cttccgatct gctatgaccg cgctgcgcgc
     1621 ggcgttagcg tcgcgcgaca atggcaggtg gagagggcat gacggtcgag cggcaggcgg
     1681 gggaggggag cgctttctcc catcgcgaga tcctccatat cctgagcggg gtccttctcg
     1741 ggatgatgct ggcggcgctc gaccagagca tcgtcgcgac cgcgctcccg gccatcgcca
     1801 gcgatctcca cggtctcgcc gatctctcct ggatcgtcgc cggctacctg ctgacctcga
     1861 cggcggcgac gccgatctac ggcaagctca gcgatctcta cgggcgccgc gcgctgctgc
     1921 aattcgccat cgtcgtgttc ctcctggcat cgctgctctg cgccgcggcg cagaacatcg
     1981 ccgagctcgt cgcggcgcgc gcgctgcaag gcgtgggcgg cggcggtctc atcagcatgg
     2041 cgcaggcggt catcgccgac gtcatctcgc cgcgcgagcg cgggcgctac caggcttaca
     2101 tgagcggcgt ctgggcgacg gcaagcgtcg gcgggccggt gctcggcggc ttcttcgtcg
     2161 atcatctaag ctggcgctgg gtgttctgga tcaacctgcc gctcggcatc gccgcgttcg
     2221 tgctgtgccg gcgcgcgctg gcgcggctcg cgacgccgcg ctcccatcgc cgcatcgatt
     2281 acctgggcgc ggtgctgctg acgctggccg tcgccgatct ccttctgctg gtgacttggg
     2341 gcggaagcac gctgccctgg acgtcgccgc aatttctctt tctgatgatg gcggcggcgc
     2401 cgctcgtctt cgccttcatc gtgcaggagc tgcgcgccgt cgatcccatc ctgccgccgc
     2461 ggctcttcgc cgatccgctg atcggctgcg ccaatctcgc gagcttcatc gtctcgatgg
     2521 cgatgttcgg cggcgttctg ctgctgcccg tgttcctcca gctcgtgacc ggcgtcggcg
     2581 ccgggcattc gggcgttctc cttatcccct tgatgggcgg aagcgtggcg ggcgctttta
     2641 cggcggggca gctcatgcgg cgcaccggcc attacaagcg ctttgcgctg atcggcctgg
     2701 tcatgagcac gctcgccttc gcgctgctcg cgaccatgga cgaggcgaca cggccggcgg
     2761 tagcgatggg ctacatggcg ctgctcggca tcggcgtcgg cagttcgatg ccggtgatgc
     2821 tggtcgcggt gcagaacgcc gcggagccgc gcgacatcgg cgtcgccacc gcgagcgtcg
     2881 ccttctgccg ctcgctgggc ggcgcgttcg gcgcggccat cctgtggtcg gtgctgctgg
     2941 gcgccttggg gcaccggctc ggtggcgcag cactcgccgg caatctgctc gaaggcgccg
     3001 atgcgacggc gctcacggcg gcgcaacgcg cggtgatggt gccggcgctg gtgccgtcct
     3061 tccacctcgt cttcgcaatc gccgcgctca tcgccgcggc gagcgtggtt gcgacgctgt
     3121 tcctgcggga gctgccgctg cgcacgacgc cgcgtgggtc ggcgcagcgt tagagcgcgt
     3181 cgtgctcgtc ggcgccgcaa ttcgtttgtg cgtccttcga caagctcagg atgaggaaga
     3241 tcggggacgg catcttccgt cgatccgagt atgaacaatc ctcatcctga gcttgtcgaa
     3301 ggacgcacag cagctactgc agcggtctat tcaaaatcga atccggaaga cccctgccct
     3361 ctcccccagg tgggggagag ggggattgca gagtttgcaa gcggggatgc tcgcaatccg
     3421 ctcggcaagc ggggcggtca tacgcgaccg cgccctccgg cgccgtggcc ggagcgatca
     3481 agcgtcgttc gagcggcggc tcagccgaag acgcggcgca actggttcca cggcttgagc
     3541 gagccgcgcc accaggcgcc aacgggcggc gaggccggaa ccggcggccg ctcggcgacg
     3601 ccatgcacga cattctcgcg cgcctggccc agcaccttga gcaccaggcc atgcgtcgtc
     3661 agcggatgat cgttgaacat ctcgatccgc agataatcgc gg
//
LOCUS       DASYNS010000292        12309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4747313, whole genome shotgun sequence.
ACCESSION   DASYNS010000292 DASYNS010000000
VERSION     DASYNS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12309
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4747313"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..286
                     /locus_tag="VGP50_08960"
     CDS             68..286
                     /locus_tag="VGP50_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027547.1"
                     /translation="MDRFGGEPTLEEVLSDRIVHLVMKADGVDMLRLCETLLKARCYA
                     ATASQDPQAMRTHAPDAETTFRRRIQTL"
     gene            complement(473..769)
                     /locus_tag="VGP50_08965"
     CDS             complement(473..769)
                     /locus_tag="VGP50_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027548.1"
                     /translation="MRPAIGVAFLALALLGAPLAAVTPVAARDDVKVSIDPNSVAFAY
                     DDGYWDRDHHWHAWRSPDEADWYRSHYHEHYTARKHDADHDQGWRDADRWWDHR"
     gene            903..1964
                     /locus_tag="VGP50_08970"
     CDS             903..1964
                     /locus_tag="VGP50_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8027549.1"
                     /translation="MQGTPGSLHERFVGDLFRRTMRLARSALFVLLGASPALATEAPT
                     YGPELEGFSYPFPVQHYAFTSQGNALRMAYMDVRPRTPNGHTVVLLHGKNFCAATWEE
                     TISILRDAGYRVIAPDQIGFCKSSKPQGYQFSFQQLAGNTRALLQSLGVEKAVISGHS
                     TGGMLAIRYALMYPESTEQLVLTNPIGLEDWKAKGVPWQSVDQWYEREKQTTDARIRA
                     YEQTTYYAGAWRPDYERWVQMLAGMYRGPGRDAVAWDSALLYDMIYTQPVYYELEQIK
                     VPTLLLIGDKDTTAIGKDLAPPEIRASLGNYPLLAKEAAKRMPKARLVEFPDLGHAPQ
                     IQDPPAYHKALLEGLTAAR"
     gene            2022..4103
                     /locus_tag="VGP50_08975"
     CDS             2022..4103
                     /locus_tag="VGP50_08975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV8027550.1"
                     /translation="MTEALHTRHAGEAISADLPSRDLGRAWPWLGLSGVVVLALTLRC
                     WALERNGYGNEYYSAGVRSMLESWHNFFFNSFDPAGFVSLDKPPVAFWIQAASAKLFG
                     FSGLSVLLPQALEGVAAIVLLFHIVARRFGAACGLLAALFLALTPVSVAVDRSTNTES
                     CLVLVLLLSGWALSLAAERGSWRLLLLSMALLGIGFNVKMLAALVVVPSFLLVYGLGA
                     PLALRTRFVHGVAALLVLAAVSLSWSVAVDATPAAARPFAGSSQGNSMLELAFDHNGL
                     QRFIHGSRRLLGGAPTVASAQPGAAAPLPASPPAAGQAAPTTRQRPDTVPAGPLRLVS
                     PFLAGQVGWLLPLAVLGLIVAVPKSRPVLPLLPPAQAILLWGGWAASYAAIFSGAGGI
                     FHAYYLATLAPPLAALAALGVTSLWRRHRSGGRSSLVLPGALLATAAWQYAVAARFPG
                     ATDVAWWPWLGLALIFGVVLSILALLAPVPGQARLAGFGVGVVAILAMPAAWASSNVV
                     FASHVEFPYAGAWRSTGPDDPALRPVRARQIADLLQFLREQRRGERFLVATPDARRAA
                     PLIIASGEAVMAMGGYMGADSILTPEALSLMVQRRDVRFVMVVPEDEAAQRTDHGTAL
                     AAWVRQNGKLVDPTLWHAVRDQPADIDPGAPPRERSARHSPRNLASLEIYDLSPPDDE
                     PARPSVRGGER"
     gene            4318..4791
                     /locus_tag="VGP50_08980"
     CDS             4318..4791
                     /locus_tag="VGP50_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV8027551.1"
                     /translation="MSGISHNSKTSAVSTLSAAFDRLAELSWPLVRISTGLLLLPHGS
                     QKLFGWFGGYGLAGTGQFFDTLGLHPGFVIALLAGVVEFFGGLALVFGLLTRPASLAV
                     AVLMGVAIFRVHLPNGFFWTDGGFEYPLLWGLIAIAIFLRGGGRFSLDARFRLPL"
     gene            4838..5536
                     /locus_tag="VGP50_08985"
     CDS             4838..5536
                     /locus_tag="VGP50_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEV8027552.1"
                     /translation="MIIVRPSEERGKADHGWLDSRHTFSFADYDDRRHRGFGALRVIN
                     EDRVAPGAGFGTHGHRDMEIISYVLDGAIEHKDSIGTGSVIRPGDVQRMTAGTGVRHS
                     EFNASKTEPVHFLQIWIIPDTVGLKPGYEQKSFPLADRTDRLRLVASQDGRDGSLTVH
                     QDVSLYATVLTPDAKVRLSLPKDRHVWVQVTRGDATANGQTLAQGDGAAIFDQQEIEF
                     SSLGGGEILVFDLA"
     gene            complement(5607..6377)
                     /locus_tag="VGP50_08990"
     CDS             complement(5607..6377)
                     /locus_tag="VGP50_08990"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEV8027553.1"
                     /translation="MWARIDRELAAIEQQHHVRILFAVESGSRAWRFPSIDSDYDVRF
                     VYAHAAAAYLSIEPPDDVIERPTDGILDVNGWDVRKLLQLLVRSNAVVLEWLTSSIRY
                     RDGGPIAAQLLALARETCYLPALAYHYDRLARNSFGEITSSPGAVRFRAYCYALRPAL
                     ALLWVRRGEAPPMDLWSLLAGVAIPEALRHTVAEFVERKAAADERGMTARLPRLDAFL
                     DEVLSEPENRFVLPEREAVRLSADELFRSILRLSLSSD"
     gene            complement(6411..7511)
                     /locus_tag="VGP50_08995"
     CDS             complement(6411..7511)
                     /locus_tag="VGP50_08995"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEV8027554.1"
                     /translation="MEGPGAQRREGEGAPAARPPFVYSDTSRHLTCIVPSQKKTSMDL
                     IVEMRFGSHLYGTDTAQSDLDLKAVYLPSADDILLQRVKASIVGPHSKALGEKNAPGD
                     VDRETYSLQKYLELLAEGQTVALDMLFAPDSAMTVPPKPLWREIQANSDRLVSRRAGA
                     FVRYCRQQANKYGIKGSRVASARKALAILAAAEARIGTTAKLERIAAELADFAGTEHI
                     ALLDLPMPGGRLIRHLEVCGRKISFTASMKSACDIVRRLVDEYGQRALEAERNEGIDW
                     KALSHAVRVGREAIELFSTGRITLPLNDADHIRRIKSGEIPYEAVAAEIERLLDDVVA
                     AAAVSRLQEEADRAVIDDIVARAYRDKVVGRV"
     gene            complement(7546..8589)
                     /locus_tag="VGP50_09000"
     CDS             complement(7546..8589)
                     /locus_tag="VGP50_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEV8027555.1"
                     /translation="MSETPRWKHRPSDSTWGDFGADDQLGRLNLLTPQKVLQGLAEVK
                     HGLAFCLSLPLDYPGDNKLNPRRYAPELRPTMRGDNPIMNYPIAQNDPRFTDVVSDDA
                     VLLHLQYSTQWDSLAHVGQMFDADGDGKPEMVYYNGYRAGEDVIGPVDYATGKKVEAP
                     VGARRLGIENMAAKAVQGRAVMIDLEAHFGRDRKLVGFAELMQVLETDDVTVEPGDMV
                     CLRTGFAQVVLDMKRKPDEKVLHNSCAVLDGRDARLLDWITRSGLSVLVADNYAVEGL
                     PASPCTEDRCAALPLHEHCLFKLGVHLGELWHLSELADWLRERRRSRFLLTAPPLRLP
                     GAVGSPVTPVATV"
     gene            complement(8599..9168)
                     /locus_tag="VGP50_09005"
     CDS             complement(8599..9168)
                     /locus_tag="VGP50_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8027556.1"
                     /translation="MRALFGLIAPRAGRVLFAERDVTGRRPAENVADGLAFIPQGHGI
                     FRTLSVRDNLELGAFALKERSRLAERLDTVFALFPVLAERARQRGGTLSGGQQQMLAI
                     GMALMHAPRLMILDEPSLGLAPNLVDKVMEAIRTVNRRFGTTILMVEQNVRATLPIAD
                     RVCVLKTGLKIYDGPPGPLHDRVELMKFF"
     gene            complement(9307..10992)
                     /locus_tag="VGP50_09010"
     CDS             complement(9307..10992)
                     /locus_tag="VGP50_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEV8027557.1"
                     /translation="MGYLLHILVMVSLYAILAVSFNLLIGFAGLFALSQAAFFGVGAY
                     TTAILATKLDLAFPLPLLAGVLLTAGLGILVALPALRIGGDHLVVVTLALQIIAVAVM
                     VNWRSVTGGTDGIAGIPKIVLWGTALDSPGRFLPFALAVAAASVALAWRLAASPFARA
                     VRAMRENELAAQAVGKNVVAIKLAVFAFSAALAAVAGGLYAHYIGFVSAETFTLDLTI
                     YVLAMVILGGTGNLWGSLLGALILTALPELLKFVALAPDIADKSRQILYGLALIAILL
                     LRPQGLLGEIPTRRRRVVVEGHGPAPLGATAPADGRVVVEGRGLMRRFGGIVAVSGLD
                     IELRRGRIVGLIGPNGAGKTTAFNLLTGFLRPDAGSVTLNGVPLAGARPHQIVRRGMA
                     RSFQDLKLFSRMTVLDNVMVALPRQKGDNPLYVYLAPWIVAAEERANRQRALAILDFV
                     GLGERAGDIAENLSYAEEKLLVIARLVATGAEVLLFDEPLSGLAPNTLEAIFPIIRRL
                     AESGKAICIIEHNLDVIRGLCHTAVFLDEGRKLVEDAPEKLIADPALAARYFG"
     gene            complement(11003..11872)
                     /locus_tag="VGP50_09015"
     CDS             complement(11003..11872)
                     /locus_tag="VGP50_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8027558.1"
                     /translation="MDLVAQLFVDGVINGSHYTLLGLGFGLIFGPTRITHFAFGPLYA
                     LAAYACWTAAVPLNLPLPVAAAVGVAAGALSGVVTYRVLYRPFERRQSASLVVLIASL
                     GLFIVLENAIGVVFGSDTKVVARFNADVILWGDVVFTSVQAWQVAALVVIGTLLALFL
                     RYSDYGKAIVAMTDNPEMARIVGIDTVRLTTLVFALGSAIAAVPAVLILLKEGATPYM
                     GFSAVFMGFVAVIVGGIGSLRGAVIGGLALGLVENLGAWQIPTEWQSTIAFVVLFLVL
                     LLKPRGLFGRGAR"
     gene            complement(12029..>12309)
                     /locus_tag="VGP50_09020"
     CDS             complement(12029..>12309)
                     /locus_tag="VGP50_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027559.1"
                     /translation="KKRYGADPDFYAANYYENTYVIAECIRRARAKGGDYWDGAKLRD
                     ALLANPRFASVYGGDMVFQPNGVALKRTALFTVEGGKRKFVKFFETTQ"
BASE COUNT         2086 a         4061 c         4137 g         2025 t
ORIGIN      
        1 ggtaacaggg atatcggatt gcccctccgc ctataacggc agagacgtac aacccggata
       61 agcagcaatg gaccggttcg gtggtgagcc aacgctcgaa gaggtcctct ccgatcgcat
      121 cgttcatctg gtcatgaagg ccgatggggt ggacatgctg cgcctttgcg aaacgctgtt
      181 gaaagcccgc tgctacgcgg ccaccgcgtc gcaggatcct caagccatgc ggacacacgc
      241 gccagatgcc gagacgactt tccggcgcag aattcagact ttatgacact ggtggtcaat
      301 cggtcaggat gaggtgactc gccgagccgg cgggaccgtg gcaccgttga acggcgtgtg
      361 gccgggtgcc gctctgtccg tcaccgggtc tcgttctcgc ccgtggcgct ctcggcgcgc
      421 gcaaaaaaag cccgggatga tcccgggctt ttgccaatgg tctggtgttg cgtcagcggt
      481 gatcccacca gcggtccgcg tcgcgccacc cctgatcgtg atccgcatca tgcttccggg
      541 cggtgtagtg ctcgtggtaa tgcgagcggt accagtcggc ttcgtcgggg ctgcgccagg
      601 cgtgccagtg atgatcgcgg tcccagtagc cgtcgtcgta ggcgaaggcg acgctattgg
      661 gatcgatcga gaccttgacg tcgtcgcgcg ccgccaccgg cgtgacggca gccaagggcg
      721 ccccgagcag ggcaagagca agaaaagcca ctccaatcgc gggtctcatg acgttctctc
      781 cttagagctc aagaatgaac atcaaacagg agcggctgga tgcccgttcc cgcgccacag
      841 acagatgcca caaggatcat ccggtggctg gctgccactt cccttgccat agcgtgacga
      901 gaatgcaagg aacacctggc tcgctccatg agcgctttgt cggagacctc tttcgcagga
      961 cgatgcgcct cgcccgatcc gcgctcttcg tgcttctcgg cgcgtcgccg gcattggcca
     1021 cggaagcgcc gacctacggc cccgagctcg aaggcttctc ctatcccttc cccgtgcagc
     1081 actatgcctt cacctcgcag ggcaacgcgc tgcgcatggc ttacatggac gtgcgtcccc
     1141 ggaccccgaa cgggcacacg gtcgttttgc tgcacggcaa gaatttctgt gccgcgacct
     1201 gggaggagac gatcagcatt ctgcgggatg ccgggtatcg cgtgatcgcg cccgatcaga
     1261 tcggcttttg caaatcctcg aagccgcagg gataccagtt cagcttccag cagcttgccg
     1321 gcaatacgcg ggcgctgctc cagtcgctgg gcgtcgagaa ggcggtgata tcgggccatt
     1381 cgaccggcgg catgctggcc atccgctacg cgctcatgta cccggagagc accgagcagc
     1441 tggtcctgac caatccgatc gggctcgagg attggaaggc gaagggcgtt ccctggcaga
     1501 gcgtcgacca atggtacgag cgggagaagc agacgaccga tgcgcgcatc cgcgcctatg
     1561 agcagaccac atattatgcc ggcgcgtggc gccccgacta tgagcgctgg gtgcagatgc
     1621 tggccggcat gtatcgcggc cccggccgcg acgcggtcgc ctgggattcg gcactgctct
     1681 acgacatgat ctacacgcaa ccggtctatt acgagctcga gcagatcaag gtgccgacgc
     1741 tgctcctgat cggcgacaag gacacgaccg cgatcggcaa ggatctggcg ccgcccgaga
     1801 ttcgcgcaag cctcggcaat tatccgctgt tggccaagga ggcggcaaag cgcatgccaa
     1861 aggcccgcct cgtcgagttc cccgatttgg gccacgcgcc gcagatccag gatccgcccg
     1921 cctatcacaa ggccctgctc gagggactga cggccgcgcg ataggcatcc gcgccgggcg
     1981 gtgccaggga tgcatctcgc gcggcggccc tatcaccggc gatgacggaa gccctgcaca
     2041 cccggcacgc aggcgaggcc atctccgccg acctgccgtc gcgcgacctt ggccgcgcgt
     2101 ggccttggct cggcctctcc ggggtcgttg tgctcgcctt gacgctccgc tgctgggcgc
     2161 tcgagcgcaa cggctacggc aatgaatatt acagtgccgg cgtgcgcagc atgctcgaga
     2221 gctggcacaa tttcttcttc aattccttcg accccgccgg cttcgtctcg ctcgacaaac
     2281 cgccggtcgc cttctggatc caagcggcga gcgccaagct cttcggcttc agcgggctca
     2341 gcgtgctgct gccgcaggcc ctggaagggg tcgccgcgat cgtcttgctc tttcatatcg
     2401 tcgcgcgccg cttcggcgcg gcctgcgggc tgctcgccgc gctctttctc gcgctcacgc
     2461 cggtcagcgt cgccgtcgat cgttcgacca acaccgaaag ctgtctcgtc ctggttctgc
     2521 tgctgtcggg ctgggcactg agtctggccg cggagcgcgg cagctggcgg ctgctcctgc
     2581 tgtcgatggc gctcctcggc atcggcttca atgtcaaaat gctggcggca ctcgtcgtcg
     2641 tgccatcctt tctgctggtc tatgggctgg gcgcgccgct ggcgctgcgg acccggtttg
     2701 tccatggggt ggcggcgctg ctggtcctgg cggcggtgtc gctgtcctgg tccgtcgccg
     2761 tcgacgcgac gcccgccgcg gcgcggccat tcgccggcag cagccagggc aattcaatgc
     2821 tggagctcgc cttcgaccac aatggcttgc aacgtttcat ccacggcagc cggcggctcc
     2881 ttggcggcgc gccgaccgtg gcatcggcac agcccggcgc cgccgcccct ttgccggcat
     2941 cgccgcccgc tgcgggacaa gcggccccaa cgacgcgcca acggccggac accgtccctg
     3001 cggggccgct gcgcctggtg tcgccgttcc tggcgggaca ggtcggctgg ctgctgccgc
     3061 tcgccgttct cgggctgatc gtggcagtgc cgaagtcgcg acccgtacta ccgctgttgc
     3121 cgccggcaca ggcgatcctc ctatggggcg ggtgggcggc gagctacgcc gcgatcttca
     3181 gcggtgccgg cggcattttc catgcgtatt acctcgcgac cctggcgccg cccctcgccg
     3241 cgctcgccgc gctcggcgtc accagcctct ggcgccggca tcgttccggc ggccggagct
     3301 ctctggtgct gccgggcgcg ctcctggcga cggcggcgtg gcaatatgcc gtcgccgcgc
     3361 ggtttccggg tgcgaccgat gtcgcctggt ggccgtggct cggcctcgcg ctgatctttg
     3421 gcgtggtgct gtcgatcctg gcgctgctcg cgccagtgcc agggcaagcg cggttggcgg
     3481 ggttcggcgt cggtgtcgtc gccatactgg ccatgccggc ggcatgggcg tcgagcaacg
     3541 tcgtcttcgc gagtcacgtg gagttccctt atgccggagc ctggcgcagc accgggcctg
     3601 atgatccggc gctccgcccc gtccgcgccc ggcagatcgc cgatctgctt cagttccttc
     3661 gggaacagcg tcggggcgag cgttttctcg tggcaacgcc cgacgcgcgc cgcgccgcgc
     3721 cgctcatcat cgcgagcggc gaagcagtca tggcgatggg cggctatatg ggcgcggact
     3781 ccatcctgac gccggaggcg ctgtcgctca tggtccagcg ccgagacgtg cgcttcgtca
     3841 tggtcgtccc cgaagacgag gcggcccagc gcaccgatca cggtacggcg ctggccgcct
     3901 gggtgcgcca gaacggcaaa ctcgtcgacc ccacgctctg gcatgccgtg cgcgaccagc
     3961 cggcagacat cgatccaggg gcgccaccgc gggaaagatc cgcccgccat tcgccacgca
     4021 acctggcatc gctcgaaatc tacgatctgt ccccgcccga cgacgagccc gcgcgaccct
     4081 ctgtccgcgg cggagagcga tagtatctta cgggctcgtc cggccgactg cggaaacgcc
     4141 gcccgtatcg ctgcggcgac cctgctccgg gtcacggcag ctcgattgat ctatcattta
     4201 taccgatgga tgcgatcaca atttgcccag ttcaaatgat ggaccggccg acccacattt
     4261 caagagcgcc gggggtgcac agcgcattgc ggcgcgtttc cctccaaggg ggtccccatg
     4321 agcggcatct cgcataattc caaaacctcc gccgtttcga cgctgtccgc cgctttcgac
     4381 aggctcgccg agctgagctg gccgctcgtc cgcatttcga cgggcctcct gctcctcccg
     4441 catggttcgc agaagctgtt cggctggttc ggcggctacg ggctggcggg cacggggcag
     4501 tttttcgaca cgctcggact gcatccgggc ttcgtgattg ccttgctcgc cggcgtggtc
     4561 gagttcttcg gcggcctggc gctggtcttc ggccttctga cgcgacccgc ttcgctcgcc
     4621 gttgccgtcc tgatgggcgt cgccatcttc cgcgtccatc tgcccaacgg cttcttctgg
     4681 acggacggtg gcttcgagta tccgctcctc tggggcctca tcgccatcgc tatcttcttg
     4741 cgcggcggcg gccggttttc gctcgacgcc cgcttccgcc ttcctctatg acccaccctc
     4801 agacggcggc gcgtccggcg ccgccccgga gttccccatg atcatcgtcc gtccctccga
     4861 agagcgcggc aaggccgatc acggctggct cgacagccgt cataccttct ccttcgccga
     4921 ttatgacgat cgccgccaca ggggcttcgg cgcgctgcgc gtcatcaacg aagaccgcgt
     4981 cgcgccgggc gccggctttg gcacgcatgg ccaccgcgac atggagatca tctcctatgt
     5041 cctcgacggc gcgatcgagc acaaggacag catcggcacg ggctcggtga tccgccccgg
     5101 cgacgtgcag cgcatgacgg cgggcaccgg cgtccgccac agcgagttca acgcgtccaa
     5161 aaccgagccg gtgcatttcc tgcagatctg gatcattccc gacacggtcg ggctcaagcc
     5221 cggctacgag cagaagagct tcccgctcgc ggaccggacc gaccggctcc gactcgtcgc
     5281 ctcgcaagat ggacgcgacg gcagcctcac cgtgcatcag gacgtgagcc tctacgccac
     5341 cgtgctgaca cccgatgcca aagtgcggct gtcgttgccg aaagacaggc atgtctgggt
     5401 gcaggtcacg cggggcgacg cgacggcgaa cggccagacg ctcgcgcaag gcgacggcgc
     5461 cgccatcttc gaccagcagg agatcgaatt ctccagcctc ggcggcggcg agatcctcgt
     5521 cttcgacctc gcataggaga cctcagcgaa gaccgagtcc ggcggcttgg cgctcttgcg
     5581 ggcgagggat agcggtgtcg cctctatcag tcggacgaga gcgagaggcg aagaatcgac
     5641 ctgaatagct cgtccgcgct aagacgaacg gcctctcgct ccggcaacac gaaccgattt
     5701 tccggttcgg acaggacctc gtcgagaaag gcatccagtc gcggcaagcg ggctgtcatg
     5761 ccgcgctcgt cggcggccgc cttgcgctcg acgaactccg cgaccgtgtg acgcaaagct
     5821 tccgggatcg caacgccggc caacaagctc caaagatcca tgggcggcgc ttcgccgcgt
     5881 cgcacccaca gcagggccag tgccggtcga agcgcataac aatacgctct gaaccgcacc
     5941 gcgccggggg acgaggtgat ctcgccaaag ctgttgcggg caaggcgatc gtagtgatag
     6001 gccagagccg gcaggtaaca ggtttcgcgg gcgagcgcga gaagctgcgc tgcgatcggg
     6061 cctccatcgc gataccggat ggacgaggtc agccactcca gcaccacggc attcgagcgg
     6121 acgagaagct ggagcagctt gcggacgtcc cagccgttga cgtccaaaat gccgtccgtc
     6181 gggcgctcga tcacgtcatc aggcggttcg atcgaaagat acgccgccgc cgcatgcgca
     6241 tagacgaagc ggacgtcgta gtcgctgtcg atggagggaa atcgccatgc gcgactgccc
     6301 gattcgacag cgaagaggat gcgaacgtga tgctgctgct cgatcgcggc gagctcgcga
     6361 tcgatgcgcg cccacatcgc ttcgctgatg catgggtcat gggacatcgg tcaaaccctg
     6421 cccacgactt tgtcgcgata cgcacgagca acgatgtcat cgatcacggc gcgatccgct
     6481 tcctcctgca ggcgcgaaac agcggcggcc gccacgacgt cgtcaagcag gcgctcgatt
     6541 tctgccgcga cggcctcgta aggaatctct ccggacttga tacggcgaat gtgatctgcg
     6601 tcgttcagcg gtaaggtgat tcgcccggtt gaaaagagct cgatggcttc tcggccaacc
     6661 cgtacggcat gggacagcgc cttccagtcg attccctcgt tccgctcggc ctcgagcgcg
     6721 cgttgcccat actcgtcgac gagacgccgg acgatgtcgc acgcgctctt catcgacgcc
     6781 gtgaaagata tcttgcgccc acaaacttcc aagtggcgaa tcaagcggcc accaggcatc
     6841 ggaagatcga gcagagcgat atgctcggta ccggcaaaat ccgcgagttc agccgcaatc
     6901 cgctcgagct ttgccgtcgt gccgatgcgc gcctcagcgg cagccagtat tgccaaggcc
     6961 ttccgcgcgc tggcaacgcg cgaacccttg atcccgtatt tgtttgcttg ttgccggcag
     7021 tagcgaacga aggcgccggc gcgccgcgag accagccggt ccgaattcgc ctggatctcg
     7081 cgccatagcg gcttcggcgg cacggtcatc gccgagtccg gcgcgaacag catgtcgagt
     7141 gccaccgtct gaccctcggc gagaagttca agatatttct ggaggctata agtctcgcga
     7201 tcgacgtcac ccggcgcgtt cttttcgccg agagccttcg agtgcggccc cacgatgctc
     7261 gccttcaccc gttgaagcaa gatgtcgtcg gcggatggaa ggtacacggc ttttaagtcg
     7321 aggtcggatt gagccgtgtc ggtgccataa aggtgcgaac cgaaacgcat ctcgacaatc
     7381 aggtccatcg acgtcttctt ctgcgaaggc actatgcatg ttagatgacg gctggtgtcg
     7441 ctgtacacga aggggggccg agcggcgggc gccccctcac cttcccggcg ctgcgcgccg
     7501 ggcccctcca tctcccgcaa tgcgggagag ggagattggc cgccgtcaca ccgtcgcgac
     7561 gggggtgaca ggggagccga cggcgccggg gaggcgcagc ggcggcgcgg tcaagagaaa
     7621 gcgggagcgg cggcgctcgc gcagccagtc ggcgagctcc gagagatgcc agagctcgcc
     7681 gagatggacg ccgagcttga acaggcaatg ctcgtgcaag ggcagtgcgg cgcagcgatc
     7741 ttccgtgcag gggctcgcgg gcaagccttc gacggcgtag ttgtcggcga cgagaacgga
     7801 aagcccgctg cgcgtgatcc agtcgagcag ccgcgcgtcg cggccgtcga ggacggcgca
     7861 ggaattgtgc agcactttct cgtcgggctt ccgcttcatg tcgagcacca cctgggcgaa
     7921 gccggtgcgc aggcacacca tgtcacccgg ctcgaccgtg acgtcgtccg tctccagcac
     7981 ctgcatcagc tcggcgaagc cgacaagctt gcggtcgcgg ccgaaatgcg cctcgaggtc
     8041 gatcatcacc gcgcggccct gcaccgcttt cgccgccatg ttctcgatgc cgagccggcg
     8101 cgcgccgacc ggcgcctcca ccttcttgcc cgtcgcatag tcgacggggc cgatgacatc
     8161 ctcgcccgcg cggtagccgt tgtagtagac catctcgggc ttgccgtcgc cgtcggcgtc
     8221 gaacatctgg ccgacatggg cgaggctgtc ccattgcgtc gagtattgga gatggagaag
     8281 caccgcgtcg tcggagacga cgtcggtgaa gcgcgggtcg ttctgcgcga tcgggtaatt
     8341 catgatcggg ttgtcgccgc gcatggtcgg ccggagctcg ggcgcgtagc ggcgcggatt
     8401 gagcttgttg tcgccggggt aatcgagcgg aagcgacagg cagaaggcga ggccatgctt
     8461 cacttccgcc aagccttgca ggaccttctg cggcgtcaag agattgagcc gcccgagctg
     8521 gtcgtcggcg ccgaaatcgc cccaggtcga gtccgaaggg cgatgcttcc agcgcggtgt
     8581 ctcgctcata aatcctcctc agaaaaactt catcaattcg acgcggtcgt ggagcggccc
     8641 gggcggccca tcatagatct tgagcccggt cttgagcacg cagacgcgat cggcgatggg
     8701 caaggtcgca cgcacattct gctcgaccat caggatggtc gtgccgaaac gccggttgac
     8761 ggtgcggatc gcctccatga ccttgtcgac gagattgggc gcaagcccga gcgacggctc
     8821 gtcgaggatc atcaatcgcg gcgcatgcat cagcgccatg ccgatcgcca gcatctgctg
     8881 ctggccgccc gagagcgtgc cgcccctctg gcgcgcgcgc tcggccagca cgggaaagag
     8941 cgcgaagacg gtgtcgaggc gctcggcaag acggctgcgc tccttcaagg cgaaggcgcc
     9001 gagctcgaga ttgtcgcgca ccgacagcgt ccggaagatg ccatggccct gcggaatgaa
     9061 ggcgaggccg tcggcgacat tctcggcggg acgccgtccc gtgacgtcgc gctcggcaaa
     9121 gaggacgcgg ccggcgcggg gcgcgatgag gccgaagaga gcgcgcatca gcgtcgtctt
     9181 gccggcaccg ttggccgagg acgatcacca cctcgcgctc ggcaatggcg aggtcgatgc
     9241 cgcgcacgat ttccttcttg ccatagccgg cggcgagcgc ggtggcggcg aggagcggcg
     9301 tcgcgctcat ccgaaatacc gcgccgcgag cgcgggatcg gcgatgagct tctccggggc
     9361 gtcctcgacg agcttccgtc cttcgtcgag gaagacggcg gtgtggcaga ggccgcggat
     9421 cacgtcgaga ttgtgctcga tgatgcagat cgccttgccg ctctcagcga ggcgccggat
     9481 gatcggaaag atcgcctcga gcgtgttggg cgcaagcccc gaaagcggct cgtcgaagag
     9541 caggacctcg gcgccggtgg cgacgaggcg ggcgatgacg agaagcttct cctcggcata
     9601 ggagagattc tcggcgatgt cgccggcgcg ctcgccgagg ccgacgaagt ccaggatggc
     9661 gagcgcgcgc tggcgattgg cccgctcctc ggctgcgacg atccagggcg ccagatagac
     9721 gtagagcggg ttgtcgccct tctgccgcgg cagcgcgacc atgacgttgt cgagcacggt
     9781 catgcgcgag aagagcttca ggtcctggaa agagcgcgcc atgccgcggc gcacgatctg
     9841 atgcggccgc gcgcccgcaa gcggcacgcc gttgagggtc acgctccccg catcggggcg
     9901 gaggaacccg gtgagcagat tgaaggcggt ggtcttgccg gcgccattcg ggccgatgag
     9961 gccgacgatc cgcccgcgcc gaagctcgat gtcgaggccg gagaccgcga cgatgccgcc
    10021 gaagcggcgc atcaacccgc gcccttcgac gaccaccctg ccgtccgccg gcgccgtcgc
    10081 cccgagcggc gccgggccgt ggccctcgac cacgacgcgg cggcgccgtg tcgggatctc
    10141 gccgaggagc ccttgcgggc gcagcagaag gatggcgatg agcgccaggc catagaggat
    10201 ctggcggctc ttgtcggcga tgtcgggagc gagcgccacg aatttcaaca gctcgggcag
    10261 cgccgtcaga atgagcgcgc cgagcagcga gccccagaga ttgccggtgc cgccgaggat
    10321 caccatggcg agcacataga tggtgaggtc gagggtgaag gtctcggcgc tgacgaaacc
    10381 gatgtaatgg gcgtaaagac cgccggcgac cgccgccagc gccgccgaga aggcaaagac
    10441 cgcgagcttg atggcgacaa cgttcttgcc gacggcctgc gcggcgagct cgttctcgcg
    10501 catggcgcgc acggcgcggg cgaagggcga ggcggcgagc cgccaggcga gcgccacgct
    10561 cgccgcggcg acagcgagcg caaagggcag gaagcggccc gggctgtcga gggcggtgcc
    10621 ccagaggacg attttcggga tgccggcgat gccgtcggtc ccgcccgtga cgctccgcca
    10681 gttgaccatg acggcgacgg cgatgatctg cagcgcgagc gtgacgacga cgaggtgatc
    10741 gccgccgatc ctgagcgcgg gcagcgccac cagtatgccg agcccggccg tcagcagcac
    10801 gcctgccagc aagggcagcg gaaaggcgag gtcgagcttg gtcgcgagga tcgcggtggt
    10861 gtaggcgccg acaccgaaga acgccgcttg cgacaaggcg aagagcccgg caaagccgat
    10921 cagcagattg aaggagacgg cgaggatcgc atagagcgac accatcacca ggatgtggag
    10981 gagatagccc atgcccgtgc gctcagcgcg cgccgcggcc gaacaggccg cgcggcttca
    11041 gcagcaacac gagaaagagc acgacgaagg cgatggtgct ctgccattcg gtcggaatct
    11101 gccaggcgcc cagattctcg acgagcccca gcgccaggcc gccgatcacc gcgccccgca
    11161 gcgagccgat gccgccgacg atgacggcga cgaagcccat gaagacggcg gagaagccca
    11221 tataaggggt ggcgccttcc ttgagcagaa tcagcacggc cggcacggcc gcgatcgccg
    11281 agcccaaggc gaagacgagc gtcgtcaacc gcacggtgtc gatgccgacg atgcgcgcca
    11341 tctcgggatt gtcggtcatc gccacgatcg ccttgccgta atcgctgtag cggaggaaga
    11401 gtgcgaggag cgtcccgatc acgacgagcg ctgcgacctg ccacgcctgg acggaggtga
    11461 agacgacgtc gccccagagg atcacatcgg cattgaagcg cgccacgacc ttggtgtcgc
    11521 tgccgaagac gacgccgatc gcgttctcga gcacgatgaa gaggccgagc gaggcgatga
    11581 gcacgacgag gctggcgctc tgccggcgct cgaagggacg atagaggacg cgataggtga
    11641 cgacgcccga gagcgcgccg gcggcgacgc cgaccgccgc cgcgaccggc agcggcaggt
    11701 tgagcgggac cgcggcggtc cagcaggcat aggccgcgag cgcatagagc ggcccgaagg
    11761 cgaaatgcgt gatgcgcgtc ggcccgaaga tgaggccgaa gccgaggccg agaagcgtat
    11821 agtggctgcc gttgatgacg ccgtcgacga agagctgagc gacgaggtcc atcgggccta
    11881 gcttagttcg cgaggggcgg cgcgttgtca ccccccaccc taccctcccc gtcaaggggg
    11941 gagggaaatg gtgacgtcgc gcgatattcc ccctcccccc ttgaggggga gggttggggt
    12001 ggggggtgac gcggcacgga cggcgcagtt actgcgtcgt ctcgaagaac ttcacgaatt
    12061 tgcgcttgcc gccttcgacg gtgaagagcg cggtgcgctt cagcgcgacg ccgttgggct
    12121 gaaacaccat gtcgccgcca tagacgctcg cgaagcgcgg attggcgagc agcgcgtcgc
    12181 gcagcttcgc gccgtcccag tagtcgccac ctttggcgcg cgcgcgcctg atgcattcgg
    12241 cgatgacgta cgtattctcg tagtagttcg ccgcgtagaa atcgggatcg gcgccatagc
    12301 gcttcttgt
//
LOCUS       DASYNS010000293        11298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2253445, whole genome shotgun sequence.
ACCESSION   DASYNS010000293 DASYNS010000000
VERSION     DASYNS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11298
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2253445"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1272
                     /locus_tag="VGP50_09025"
     CDS             <1..1272
                     /locus_tag="VGP50_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027560.1"
                     /translation="IDWRITLVLVIIVVGLGVLIARLASRRQYLHRRFAGRAAAVTGS
                     LTDVVSNIGLVRAFGAARREKQRLSEEIGREMTAQRESLLALERLRLLHALAVFAVTA
                     GILAWSVSFWRLGLVTAGDVVLTTSLGFTVLHASRDLAMAMVELVQHFAKLGEAVQVL
                     GLPHEMEDSSDAKPLIRLGGSVSFLNVCFSYPEGERVLQDFTLHIPAGQKVGLVGRSG
                     AGKSTLMALVQRLYDPESGRVLVDEQDLTKVTQESMRQSTAVVHQDISLFHRSLLENL
                     RYGRPDASDEDVHAAAKAAHCVEFIERLPSRYDTIVGERGLKLSGGQRQRLAIARAFL
                     RHAQLILLDEATSSLDSESEQLIQEALAQLMKGRTVIAIAHRLSTLDSFDRIIVLERG
                     HIVEDGSPAELVRKKGVYAEMYRRQLAAAKR"
     gene            1272..1814
                     /locus_tag="VGP50_09030"
     CDS             1272..1814
                     /locus_tag="VGP50_09030"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEV8027561.1"
                     /translation="MRRALAATVLAALLTLGVVGARSEPPGGNAPASQAPEAAAPPNP
                     APAPPPEAKPEPEPKPPQNLEEIDAGTTVAILGKKVRDASGKDMGLVVEVLVDRDANV
                     RAAVIDFGGFLGVGSRKIAIDWRLLQFDPADRDAPIVLSLDRAEVQAAPEYKPGGQPG
                     PLVLVGPPPAVGTSAPPGGQ"
     gene            1822..3045
                     /locus_tag="VGP50_09035"
     CDS             1822..3045
                     /locus_tag="VGP50_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8027562.1"
                     /translation="MAVSERVQEREIGSTASPSAERALDWLNLFVANIQTGFGPFIAV
                     YLTTQGWTQTAIGIALSVGTVTSMASQVPAGALVDATARKSTAAAFSILAFTLSALMF
                     AFWPVPLSVYLAEVLHGFSSCTLGPAIAAISIALAGRAALGQRLGRNARYASIGSALG
                     AGLMGLCGYYVSNAAIFFLTAALTLPALAALVPLSRIDERALGPDRHSAPQRERIAHL
                     LADRRLWIFACCAMLFTLGNAGVLPLASTEITRRASSVASLLIAACIVLPQLIVALIS
                     PRVGAFAESRGRRWVMLVGFSMLPLRCLLLAFVTSPPLMVLVQALDGVAAASFGVLVP
                     LVTSDVAGRSGHYNLSLGFVGFAIGIGGTFGTPLAGFMADRFGDPVAFASLGLIGFVV
                     AILVWAAMPETRSPR"
     gene            3289..4197
                     /locus_tag="VGP50_09040"
     CDS             3289..4197
                     /locus_tag="VGP50_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV8027563.1"
                     /translation="MRGLARALAMLALIAGLPVVAGGARAGERIDRAFRECPDCPEMV
                     AIPAGKFVMGSPATERGRFDAEGPQHAVAVRAFAIGKYDVTNAQFLAFLQETAYQPAP
                     CDPVLDLTWHSPGRGIAYPPGRTDSPLEPAVCLSWNDAQAYLVWLNRKVGASLPKKRE
                     GPYRLPSEAEWEYAARAGSTTARWWGEAIGGGKANCHGCGSKWDGSLIAPVGSFGPNP
                     FGVYDMLGNVWQWVDDCWNESYVGAPADGSPWLTGDCGKRVLRGGSWSNVPEFVRSAS
                     RSRAEETGRGFDYSSYAGFRVARTLP"
     gene            complement(4205..5068)
                     /locus_tag="VGP50_09045"
     CDS             complement(4205..5068)
                     /locus_tag="VGP50_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027564.1"
                     /translation="MDRRPDRISGPLVGSALLHVLVLLLIIFGLPWPGSPVEQVIPID
                     LVFLGEKTASPPAEAVATLPQERATEASELTPDQAVPAEQAPPPQAAQRRAEERAAPD
                     LSAPDKAKPKPEAPRPVKSTKLDRQPAAKLKQQPLPADDLSVRLQQLARLRQPAPPMP
                     PSPRQQKAPGSSDVTATATDAAPALDASYGVKDFIRAQVERRWNPDSKMLKGSDWVAA
                     IHVVLRPDGSVERAEIVDDPRHHAENAYRDFAQSARNAVLLSSPLTVPPGEYDLAKDI
                     VLDFHSRQVAQ"
     gene            5277..6053
                     /locus_tag="VGP50_09050"
     CDS             5277..6053
                     /locus_tag="VGP50_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027565.1"
                     /translation="MTRSDGDVVAKVPEVTLTFWIIKIAATTLGETGGDTVTMTMNLG
                     YLTGTAIFLSALVVLAALQIAAKRFHPALYWATIIASTTAGTTMADFADRSLGIGYTG
                     GSSVLFLCVMAVLGLWYWSEGTISVTTVNRPKVEAFYWAAITFSQTLGTALGDWMADS
                     TGLGYEGGALVFAAGLAILAGLYFWTNASRVLLFWAAFILTRPLGATVGDFLDKPVSD
                     GGLALSRPIATAVIGVFIIACILLLPQRAGVHPAQAGSQR"
     gene            6081..6635
                     /locus_tag="VGP50_09055"
     CDS             6081..6635
                     /locus_tag="VGP50_09055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEV8027566.1"
                     /translation="MRILAISGSLRANSSNTAVLMAAARLAPAGTEIVLYDGLAGLPH
                     FNPDLDTDDAPAPVRALRRDIGQSDGLLISSPEYARGVPGSLKNALDWLVSSLEFPEK
                     PVALINASQRSTHADAQLRVTLTTMSARLIEAASITLPLLGRGLDADGIVADAGLSAQ
                     LRDALAQLADAIRGSADAKSEEER"
     gene            complement(6709..7353)
                     /locus_tag="VGP50_09060"
     CDS             complement(6709..7353)
                     /locus_tag="VGP50_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027567.1"
                     /translation="MSEMISVAFDEQMSGFVAFGPPLDYKEGYARGKADETSCGVKLT
                     ITVPDIAVFTTDPKTEARAEGQVLCPLLGGACVIERGTFNLLVGVIPHRPAIKDMRYR
                     LVFRTPSGRVMTLVGVKFVDDDGIFRMWRDTTTLYTKIWEGEVAPVDERAAVPVASGI
                     LRLHPLPFARQLLSFRSNGPTLVSRLVAIFRFVKLFAWRLWQVYGPKFLQTKAG"
     gene            complement(7367..9082)
                     /locus_tag="VGP50_09065"
     CDS             complement(7367..9082)
                     /locus_tag="VGP50_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEV8027568.1"
                     /translation="MSERYEAIIVGSGFGGAINACRLAKQWPGRVLVLERGKRYGLGS
                     FPRTPHAMANNFWNIGFEKRTRPSMLPAIEQRGLFDVRNFKNMDVILSAGLGGGSLIY
                     ANVFLEPPDAVFGERWPRSCRKDELQPYYRVAKSVLGSRPIPDMTDPRRRILRTEMFQ
                     RVARELGRSSTLVDINVFFGNDFKKPLDLGVQDRNRYGALQTSCVYCAECDIGCNYQA
                     KNTLDLNYLYRAENAYHAAIRTEHLVDRIVPVNARDEDDPAADGRNGYRVYYRDLSAN
                     GTARDALANRVVVSAGSLGSTELLLRCRDVFKTLPLISWQLGKHFSGNGDFLGFVVGT
                     KEPAEPNYGPVITQATDFNLFHGFERDQAFILEDASYPAFAAWFVEGVKPAIFRLPSL
                     WRALRDIWARIRGHSAGGPIGYALHDILTGDLSYNTAVLLSMGHDNSNGLMTLDGGWL
                     DLAWPTNENRRLYDATLAAGEAFRKATKGKLYFAIPTWWWPMRKNVSVHPLGGCVLSD
                     TPETGVTSAARPTFGQVHGYPNLYVADGAIVPNAVGANPTATISALSEMVAQGITGVS
                     PTADL"
     gene            complement(9090..9704)
                     /locus_tag="VGP50_09070"
     CDS             complement(9090..9704)
                     /locus_tag="VGP50_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027569.1"
                     /translation="MSATTIAAATPKTEGYGIGLLEFTLAFVLFGFFLGAALLAAETT
                     MDLDLYRTVYTIWATTALVTPALCAFALPGQSARKGALWLLFWSFAFVVYIVHAGYAY
                     FGVYDGSVREFLDGQGLFPAANNVLFTLWWAFDLFLAWFYRGSAAWVQKQRIGAHVYI
                     GGVFVVSTVVLKHGFINVLGIILTLSVLLCLVIRLDAWRRSRAG"
     gene            complement(9865..>11298)
                     /locus_tag="VGP50_09075"
     CDS             complement(9865..>11298)
                     /locus_tag="VGP50_09075"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV8027570.1"
                     /translation="QGSTGTEIMPLAVFQVLPTLFPEEFQPAGPAAGDWVEQFGFLRG
                     DPSVNEGLPYGVNISHYRPKSGAPSPIAFVGFNCAICHSAKVHTGDDNGPVILGMGNA
                     AIDLVGFGDAVKSSLLNETRLTVSSIASTYETVTHKKLGLVDKLVIDVWLSGARTAIR
                     GDFPMRGTPFSGPDVRSAALMGSGPARNEPIKETVRFLIDETPAPDGGYSKIPCLYRQ
                     DRREWAQYDGSVHDPLTRNALAALGVGASVYNLRMPGILHTIQETYLFVSALEGPHYA
                     DLVTDREGAVDTERAQRGRQVYQQYCSDCHGGPEGSDWAKGKREEQVIPASEVGTDNA
                     RVTFRYYDKLAQFILDFFPDGHPLKPKPEDLRTLPPAERGYITEPLEAVFTRAPYLHN
                     GSVPTLAQLINLTPRLPVFYRGKSEFDAAEIGITVTTKPDDKHYFRFDTSVYGNANRG
                     HDYPWAYGAPGWDKEALRDLLEYLKTI"
BASE COUNT         1742 a         3710 c         3927 g         1919 t
ORIGIN      
        1 atcgattggc ggatcacgct ggtgctggtg atcatcgtcg tcggacttgg ggtcctcatc
       61 gcgcggctgg cgtcgcgccg ccagtatctg catcgccgct tcgccgggcg cgccgcggcc
      121 gtcaccggca gtctcaccga cgtcgtctcc aatatcggcc tggtccgcgc cttcggcgcc
      181 gcgcggcgcg agaagcagcg gctctccgag gagatcggcc gcgagatgac ggcgcagcgg
      241 gagagcctcc tcgctctcga gcggctgagg ctgcttcacg cgcttgccgt cttcgcggtg
      301 accgccggca tcctcgcctg gtcggtgagc ttctggcgct tgggcctggt caccgcgggc
      361 gacgtcgtgc tgacgacgtc gctgggcttc accgtgctcc acgcctcgcg cgacctcgcc
      421 atggcgatgg tcgagctggt gcagcatttc gccaagctcg gcgaagcggt gcaggtgctg
      481 gggctgcccc atgagatgga agactcgtcc gacgccaagc cgctcatccg cctcggcggc
      541 tcggtcagct tcctcaatgt ctgcttcagc tatcccgagg gcgagcgggt gctccaggat
      601 ttcacgctcc acattccggc gggccagaag gtcgggttgg tcgggcgctc gggcgccggc
      661 aagtcgacgc tcatggcgct cgtgcagcgg ctctacgatc ccgagagcgg ccgcgtcctg
      721 gtcgacgagc aggacctgac caaggtcacg caggagagca tgcgccagtc gaccgccgtg
      781 gtgcatcagg acatctcgct cttccatcgc tcgctcctcg agaatctgcg ctatggccgg
      841 cccgatgcga gcgacgagga cgtccatgcc gcggccaagg ccgcgcattg cgtcgagttc
      901 atcgagcgct tgccgagccg ctacgacacc atcgtcggcg agcgcggcct caagctttcg
      961 ggcggccagc gtcagcgcct cgccattgcc cgcgctttcc tgcgccatgc ccagctcatc
     1021 ctcctcgacg aggcgacctc ctcgctcgac agcgagtccg agcaactgat ccaggaagcg
     1081 ctggcgcagc tcatgaaagg gcggaccgtg atcgccattg cccatcgcct gtcgacgctc
     1141 gactcattcg atcgcatcat cgtgctcgag cgcggacaca tcgtcgagga cggctcgccc
     1201 gccgagctgg tgcgaaaaaa gggcgtctat gccgagatgt atcgccgcca gctggcggcg
     1261 gcaaaacgct aatgagacgc gcgctcgccg ccaccgtcct cgcggcgctg ctgacgctgg
     1321 gcgtcgtcgg cgcgcgttcg gaaccgccgg gcggcaacgc gccggcgtcg caagcgcccg
     1381 aggctgccgc gccgccgaac cctgcgccgg caccgccccc ggaggcgaag ccggagccgg
     1441 agccgaagcc gccgcaaaat ctcgaggaga tcgatgcggg cacgaccgtc gccattctcg
     1501 gcaagaaggt gcgcgacgcc tcgggcaagg acatggggct ggtcgtcgaa gtgctcgtcg
     1561 atcgcgatgc caatgtgcgc gccgccgtga tcgatttcgg cggcttcctc ggcgtcggca
     1621 gccgcaagat cgccatcgac tggcggctcc ttcagttcga ccccgccgac cgcgacgcgc
     1681 cgatcgtgct cagtctcgat cgcgccgagg tccaggcggc gcccgaatac aagcccgggg
     1741 gccagcccgg gccgctcgtt ctcgtcgggc cgccgccggc ggtcgggacc agcgctccgc
     1801 cgggaggaca atgatcgcgg catggctgtt tcggagcggg tgcaggagcg cgagatcggc
     1861 tcgacggcgt cgccatccgc cgagcgcgcc ctcgactggc tcaatctctt cgtcgccaac
     1921 atccagaccg gcttcgggcc gttcattgcc gtctatctga cgacgcaagg gtggacgcag
     1981 acggcgatcg gtatcgccct cagcgtcggc acggtcacct cgatggcaag ccaggtgccg
     2041 gccggcgcgc tggtcgacgc cacggcgcgc aagagcaccg cggccgcctt cagcatcctc
     2101 gccttcacgc tgagcgcgct catgttcgcg ttctggccgg tgccgctctc ggtctatctc
     2161 gccgaggtgc tgcacggctt ctcgagctgc accttgggcc cagcgatcgc cgccatcagc
     2221 atcgccctgg ccggccgcgc ggccctcggt cagcgcctcg gccgcaacgc gcggtatgcc
     2281 tcgatcggca gcgctctcgg cgcgggcctg atgggcctct gcgggtacta tgtgtcgaac
     2341 gccgcgatct tcttcctgac cgccgccctg acgctcccgg cgctcgccgc cttggtgccg
     2401 ctgtcgcgca tcgacgagcg ggcgctgggg cccgaccgcc attctgcgcc gcagcgggag
     2461 cgcatcgccc atctgctcgc ggaccggcgt ctctggatct tcgcctgttg cgcgatgctg
     2521 ttcacgctcg gcaatgccgg cgtgctgccg ctcgcgagca ccgagatcac gcggcgggcg
     2581 agcagcgttg ccagcctgct gatcgccgcc tgcatcgtgc tgccgcagct gattgtggcg
     2641 ctgatctcgc cgcgcgtcgg cgccttcgcc gagagtcgcg gccggcgctg ggtgatgctg
     2701 gtcggcttct cgatgctgcc gctgcgctgc ctgctcctgg ccttcgtgac gagtccaccg
     2761 ctcatggtgc tggtacaggc gctcgacggc gtcgccgccg cctccttcgg cgtgctggtg
     2821 ccgttggtca cgagcgacgt cgccggccgc tccggccatt acaacctctc gctcggtttc
     2881 gtcggcttcg ccatcggcat cggcggcacc ttcggcacgc cgctcgccgg cttcatggcc
     2941 gaccgcttcg gcgatcccgt cgccttcgcc agcctcggcc tgatcggctt cgtcgtcgcg
     3001 atcctggtct gggccgccat gccggagaca aggtccccac gctgaggcga acgcgcttcg
     3061 cgttcgtcct cggcgggcgg cagcggttgc tcttccaacc gcgagagccc gcacagcgca
     3121 ggaataggtc ctgatgggag cggcttccgc gatcgcaaga gcaatcgctg gcgccgccgc
     3181 ggacgaacgc gcgattcgcg ttcgccgtcg cgcgttcgcc gttgcaatag ccgccgccgg
     3241 acatcatggt ggcgcgcgca tggcgggcct actggcgacg gcaatcgaat gagagggctt
     3301 gcacgcgccc tggcgatgct cgcgctcatc gccggcctcc ctgtcgtggc gggcggcgcg
     3361 cgtgccggcg agcggatcga ccgcgcgttc cgggaatgcc cggattgccc ggagatggtg
     3421 gcgatccccg cggggaaatt cgtcatgggg agtccggcga ccgagcgcgg gcgcttcgat
     3481 gccgaaggtc ctcagcacgc cgttgccgtg cgtgccttcg cgatcggcaa gtatgacgtc
     3541 acgaacgccc agttcctggc ctttctgcag gagaccgcct accagccggc gccatgcgat
     3601 cccgtcctcg acctgacctg gcactcgccg ggccgcggca ttgcctatcc gcccggtcgg
     3661 accgattcgc cactcgagcc ggcggtctgt ttgagctgga acgatgccca ggcttatctg
     3721 gtctggctca atcgcaaagt cggggcgtca ctgcccaaga agcgcgaagg cccgtaccgg
     3781 ctgcccagcg aagcggaatg ggaatatgcg gcgagggcgg ggtcgacgac agcgcgctgg
     3841 tggggcgagg cgatcggtgg cggcaaggcc aattgtcacg gctgcggcag caaatgggac
     3901 ggctcgctga tcgcgccggt cggcagcttc ggccccaatc ccttcggcgt ctacgacatg
     3961 ctgggaaatg tctggcaatg ggtggatgac tgctggaacg agagctatgt cggagcgccg
     4021 gcggatggca gcccgtggct caccggcgat tgcggcaagc gcgtgctgcg cggtggctcc
     4081 tggagcaacg tgcccgaatt cgtgcgctcg gccagccgca gcagagcgga ggagaccggc
     4141 cgcgggttcg actattcgag ctatgccggc ttccgcgtcg cgcggacgct tccctgaccg
     4201 ccgatcactg cgcgacttgc cgtgaatgga agtcgaggac gatgtccttg gcgagatcgt
     4261 attcgccagg cggcacggtg agaggggagg acagcaagac ggcattgcgc gcgctctgcg
     4321 cgaagtcacg ataggcgttt tcggcatggt gacgcggatc gtcgacgatc tcggcgcgct
     4381 cgacgctgcc atccgggcgc agcacgacgt ggatcgccgc cacccagtcg ctgcccttca
     4441 gcatcttgct gtccggattc cagcgacgct cgacctgggc gcggatgaag tccttgacgc
     4501 cgtagctcgc gtcgagcgcc ggcgcagcat cggtcgccgt cgccgtcacg tcggacgagc
     4561 ccggcgcctt ctgctggcgc gggctgggcg gcatcggcgg ggccggctgc cgcaggcgcg
     4621 cgagctgctg gagccggacg ctcaaatcgt cggccggcaa gggctgctgc ttcagcttgg
     4681 ctgccggctg ccggtcgagc ttggtcgact tgaccggccg gggagcctcg ggcttgggct
     4741 tcgccttgtc cggcgccgac agatcgggcg cggctctttc ctcggcgcgg cgttgcgccg
     4801 cctggggcgg cggcgcttgt tcggccggaa cggcctggtc gggcgtcagc tccgacgcct
     4861 ccgtcgctct ctcctgaggc agcgtcgcga ccgcttccgc cggaggcgag gccgtcttct
     4921 cgccgaggaa gacgaggtcg atcggtatga cctgctcgac gggcgatccc ggccagggca
     4981 agccgaagat gatcaggagc aacaccagga cgtgcaggag agcggagccg acgagaggcc
     5041 cgcttatccg gtccggtcgg cgatccacct ccggactcgt gtcacttccg gtcgccatgt
     5101 cgtcgtcgga catctgatgc gttgcaccgc ctcttgcaag cgcaggaacg ctcgccgctg
     5161 cccgccatcg ccctgtaggt tggagtatgc catgaatgcg ctaccggccg gcgtgccatg
     5221 cgcgcgtcag gtccgcggat cgttctggcg acggaccggg tcgcaggaga ggacacatga
     5281 cacgctctga cggtgacgtt gtggcgaaag ttcctgaagt cacgctcacc ttctggatca
     5341 tcaagatcgc cgcgacgacc ctcggcgaga cgggcggcga caccgtcacc atgacgatga
     5401 atctgggcta tctgacgggc acggcgatct ttctctcggc gctcgtcgtg ctggcggccc
     5461 tgcagatcgc ggcgaagcga ttccacccgg ccctctactg ggccaccatc atcgcctcca
     5521 ccacggccgg cacgaccatg gccgattttg ccgaccgctc ccttggtatc ggctacaccg
     5581 gcggatcatc ggtcctgttt ctgtgcgtga tggccgtgct cggcctctgg tactggtcgg
     5641 aggggacgat ctcggtcacc acggtcaaca ggccgaaagt ggaggccttc tactgggccg
     5701 ccatcacctt ctcgcaaacc ctgggcacgg ctctcggcga ctggatggcc gactcgaccg
     5761 gtctcggcta tgagggcggc gcgctcgtct tcgccgccgg cctcgcgatc ctcgccggtc
     5821 tctacttctg gaccaacgcg tcgcgggtgc tgctgttctg ggcggcgttc atcctgacgc
     5881 ggccgctcgg tgccacggtc ggcgacttcc tcgacaaacc ggtcagcgac ggtggcctcg
     5941 cgctcagtcg tccgatcgcc accgcggtca tcggcgtctt catcatcgct tgcatcctgc
     6001 tcctgccgca gagagcgggc gtgcatcccg cgcaagcggg gtcgcagcga tagatccggc
     6061 gtcgggctat catggcgggg atgcgtattc tcgcgatctc ggggagcctc cgggcgaact
     6121 cctcgaacac cgcagttcta atggcagcgg caaggctcgc accagccggc acggagatcg
     6181 tcctctatga cgggctcgcc ggcctgccgc atttcaaccc cgatctcgat acggacgatg
     6241 cgccggcgcc ggtgcgcgcg ctgcggcgcg acatcgggca gagcgacggg ctcttgatct
     6301 cgagcccgga atatgcccgc ggcgtgccgg gcagcctgaa gaacgcgctc gactggctgg
     6361 tgagcagcct cgaatttccc gagaagccgg tggcgctcat caatgcgtcg cagcggtcga
     6421 ctcatgccga tgcgcaattg cgcgtgacat tgacgacgat gtcggcgcgg ctgatcgaag
     6481 cggcatcgat cacgcttccc ttgctcgggc gcggtctcga tgccgacggc atcgtcgcgg
     6541 atgccgggct ctcggcgcag cttcgcgacg cgcttgcgca gcttgccgac gcgatccgcg
     6601 ggagcgccga cgcaaaaagc gaagaggaac gctgagggcg ctgaggtttc gccgaggacg
     6661 cagaggcaga atttcctttg cgcgctggat gtttgatcga gtcccgcgct atccggcctt
     6721 cgtctgcaga aacttcggtc cgtagacctg ccacagccgc catgcgaaga gcttgacgaa
     6781 gcggaagatg gcgacgaggc gcgacaccag agtcggcccg ttggagcgga aggagaggag
     6841 ctggcgcgca aaggggaggg ggtgcaagcg cagaatgccc gacgccaccg gaacggccgc
     6901 gcgctcgtcg accggtgcga cctcgccttc ccagatcttg gtgtagagcg tcgtcgtgtc
     6961 gcgccacatg cggaagatgc cgtcatcgtc gacgaatttg acgccgacca gcgtcatcac
     7021 ccgccccgac ggtgtgcgga agaccaggcg atagcgcatg tccttgatgg ccgggcggtg
     7081 cgggatgacg ccgacgagca gattgaacgt gccgcgctcg atgacgcagg cgccgcccag
     7141 cagcgggcac agcacctggc cctcggcgcg cgcctcggtt ttggggtcgg tcgtgaagac
     7201 ggcgatgtcg ggcaccgtga tggtgagctt gacgccgcag ctcgtctcgt ccgccttgcc
     7261 gcgcgcgtag ccttccttgt aatcgagcgg cgggccgaag gcgacgaagc cgctcatctg
     7321 ctcgtcgaag gccacggaaa tcatctcgct cacggacaga cctccgtcag aggtcggcgg
     7381 tcggcgacac gccggtgatg ccttgggcga ccatctcgga gagcgccgag atcgtcgccg
     7441 tcgggttggc gccgacggca ttgggcacga tcgcgccgtc cgcgacatag agattcggat
     7501 agccatggac ctggccgaag gtcggtcgcg ccgcgctggt gacgccggtc tcgggcgtgt
     7561 cgctcagcac gcagccgccc aagggatgga ccgagacatt cttgcgcatc ggccaccacc
     7621 aggtcggtat ggcgaaatag agcttgccct tggtcgcctt gcggaaggcc tcgcccgcgg
     7681 ccagcgtcgc gtcatagagg cgccggttct cgttggtcgg ccaagccaga tcgagccagc
     7741 cgccatcgag cgtcatcagg ccgttcgaat tgtcatggcc catggagagc agcacggcgg
     7801 tgttgtagga gagatcgccc gtcaggatgt cgtgaagcgc atagccgatc ggcccgccgg
     7861 cgctgtggcc gcggatccgc gcccagatgt cgcgcagcgc gcgccacagg ctcggcaagc
     7921 ggaagatcgc cggcttgacg ccctcgacga accaggcagc gaaagcggga tagctcgcgt
     7981 cctcgaggat gaaggcctgg tcgcgctcga agccgtggaa gagattgaag tcggtcgcct
     8041 gggtgatgac cgggccgtaa ttgggctcgg ccggctcttt ggtgccgacg acgaagccga
     8101 gaaagtcgcc attgcccgaa aagtgcttgc cgagttgcca ggagatcagc ggcagcgtct
     8161 tgaagacgtc gcggcagcgc agcagcagct cggtcgagcc gaggcttccg gcggacacga
     8221 cgacgcgatt ggcgagggcg tcgcgggcgg taccgttcgc ggagaggtcg cggtagtaga
     8281 cgcggtagcc gttgcggccg tcggccgccg ggtcgtcctc gtcgcgcgcg ttgacgggaa
     8341 cgatgcggtc gacgagatgc tcggtccgga tcgccgcgtg gtaggcgttc tcggcgcggt
     8401 aaaggtagtt gaggtcgagc gtgtttttgg cctggtagtt gcagccgatg tcgcattcgg
     8461 cgcagtagac gcaggaggtc tgcagcgcgc cgtagcggtt ccggtcctgg acgccgaggt
     8521 cgagcggctt cttgaagtcg ttgccgaaaa agacgttgat gtcgaccagc gtcgagctgc
     8581 ggccgagctc gcgcgccact ctctggaaca tttcggtgcg caggatgcgc cggcgcggat
     8641 cggtcatgtc gggaatgggc cgcgacccca gcactgactt ggcgacgcgg taatagggct
     8701 ggagctcgtc cttgcggcag gagcgcggcc agcgctcgcc gaagacggca tcgggcggct
     8761 cgagaaagac attggcatag atcagcgacc cgccgcccag cccggcggag aggatgacgt
     8821 ccatgttctt gaagttgcgc acgtcgaaga ggccgcgctg ctcgatcgcc gggagcatgc
     8881 tcggccgcgt ccgcttctcg aagccgatgt tccagaaatt attcgccatg gcgtgcggtg
     8941 tgcgcgggaa ggagccgagc ccgtagcgct tgccgcgctc gaggacgagc acgcgccccg
     9001 gccattgctt ggcgagccgg caggcgttga tggcgccgcc gaaaccgctg ccgacgatga
     9061 tcgcctcgta gcgctcgctc atcgcgtctt cagccggccc ggctgcgtcg ccacgcgtcg
     9121 aggcggatga cgaggcagag gaggacgctg agcgtcagga tgatgcccag cacattgatg
     9181 aagccgtgct tcaggacgac cgtcgagacg acgaacacgc cgccgatata gacatgcgcg
     9241 ccgatccgct gcttctgcac ccaggcggca gagccgcgat agaaccaggc gaggaaaaga
     9301 tcgaaggccc accacagcgt gaacagaaca ttgttcgccg cggggaagag gccctggccg
     9361 tcgaggaatt cgcggacgct gccgtcatag acgccgaaat aggcatatcc cgcatggacg
     9421 atgtagacga cgaaggcgaa gctccagaag agcagccaga gcgctccctt gcgcgcggat
     9481 tggccgggga gcgcgaaagc gcagagcgcc ggcgtgacca gcgccgtcgt cgcccagatg
     9541 gtgtagacgg tgcgatagag atcgagatcc atcgtcgtct cggcggcgag caaggcggcg
     9601 ccaaggaaga agccgaagag cacgaaagcc agcgtgaact cgagcaggcc gataccgtag
     9661 ccttccgtct tcggcgtcgc tgcagcgatg gtcgttgcgc tcatggtggg ttccccaggg
     9721 ccaagtctcg cgcgagcgag cacatacccc ccaccctgac cctccccctc aaggggggag
     9781 ggaaatgcaa gcctggccgc tctttcttct ccctcccccc ttgaggggga gggatggggt
     9841 ggggggtcgg ccgtggcttc attgctagat cgtcttcaga tattcgagca ggtcgcggag
     9901 cgcttctttg tcccagcccg gggcgccata ggcccaggga tagtcgtggc cgcggttggc
     9961 attgccatag acgctcgtgt cgaagcggaa ataatgcttg tcgtcgggct tggtggtgac
    10021 ggtgatgccg atttcggccg cgtcgaactc gctcttgccg cgatagaaca cggggagccg
    10081 cggcgtcagg ttgatgagct gcgccagtgt cggcacgctg ccattgtgca gatagggggc
    10141 gcgcgtgaag accgcctcga gcggctcggt gatgtagccg cgctcggccg gcggcagggt
    10201 tcgcaggtct tcgggcttcg gcttcagcgg atggccgtcg gggaagaagt cgagaatgaa
    10261 ctgcgccagc ttgtcgtagt agcggaacgt gacgcgcgca ttgtcggtgc cgacttcgct
    10321 ggcgggaatg acttgctcct cgcgtttccc cttggcccag tcgctgccct cgggtccgcc
    10381 atgacagtcg ctgcagtatt gctgatagac ctggcggccg cgctgagcgc gctcggtgtc
    10441 gacggcgccc tcgcggtcgg tgacgagatc ggcgtaatgc ggcccttcga gcgcgctgac
    10501 aaagagatac gtctcctgga tcgtatgcag gatgccgggc atgcggagat tgtagaccga
    10561 ggcgccgacg cccagcgccg ccagcgcgtt acgcgtcagg ggatcgtgga cgctgccgtc
    10621 atattgcgcc cattcgcgcc ggtcctggcg atagaggcag gggatcttcg agtagccgcc
    10681 gtcgggcgcc ggcgtctcgt cgatgaggaa gcgcaccgtc tccttgatcg gctcgttgcg
    10741 cgccgggccg ctgcccatca aggcggcgct tctgacgtcg ggcccgctga agggcgtgcc
    10801 gcgcatggga aagtcgccgc ggatggcggt gcgcgcgccg ctcagccaga catcgatgac
    10861 gagcttgtcg acgaggccga gcttcttgtg cgtcaccgtc tcataggttg acgcgatgct
    10921 cgagaccgtg agccgcgtct cgttgagcag cgagctcttg acggcatcgc cgaagccgac
    10981 gaggtcgatc gccgcattgc ccatgccgag aatgacaggc ccgttgtcgt cgccggtatg
    11041 gaccttcgcg ctgtgacaga tcgcacaatt gaagccgacg aaggcgatgg gagaaggtgc
    11101 cccggatttc ggccggtaat ggctgatgtt gacgccgtaa ggcagcccct cgttgacgct
    11161 ggggtcgccg cgcaaaaagc cgaactgctc gacccagtcg cccgccgccg gacccgcggg
    11221 ctggaactct tcggggaaga gcgtcggcag gacctggaag acggcgagcg gcatgatctc
    11281 ggtgccggtc gagccctg
//
LOCUS       DASYNS010000294         4711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4593666, whole genome shotgun sequence.
ACCESSION   DASYNS010000294 DASYNS010000000
VERSION     DASYNS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4593666"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VGP50_09080"
     CDS             <1..516
                     /locus_tag="VGP50_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV8027571.1"
                     /translation="RFIYALGIPQVGQQTAKLLARHYRSFARWQEAMTAAQDQESDAW
                     RELNDIHGIGEDMAADIVGFFAERHNRALLDELRREVDVTDYASGPSAANAPLTGKTI
                     VFTGTLTAMSRSEAKARAEALGANVAASVSAKTDYLVVGADAGSKAEKARALGVTTMT
                     EEEWLALARGG"
     gene            complement(585..758)
                     /locus_tag="VGP50_09085"
     CDS             complement(585..758)
                     /locus_tag="VGP50_09085"
                     /inference="COORDINATES: protein motif:HMM:NF027514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV8027572.1"
                     /translation="MTLAPIDRGLVDAALLDAAEIAWLDAYHARVAAEIAPLVDAATR
                     RWLAAVTRPIRAA"
     gene            977..1507
                     /locus_tag="VGP50_09090"
     CDS             977..1507
                     /locus_tag="VGP50_09090"
                     /inference="COORDINATES: protein motif:HMM:NF040504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family B"
                     /protein_id="HEV8027573.1"
                     /translation="MVRIATEADADAIASIYEPYVRETAISFEEMPPTPVEMAHRLRS
                     TRDTYPYLVFDDGDGVLGYAYGSVHRAKPAYRWSVETTLYVARSAHRRGIGRALYTEL
                     LHLLTRQGFHSAFAGIVPPNEKSVGLHESMGFVYLGTFVEIGYKHGELHHLGWWRRTL
                     KEGRPQHEPIPFTSLV"
     gene            complement(1832..3871)
                     /locus_tag="VGP50_09095"
     CDS             complement(1832..3871)
                     /locus_tag="VGP50_09095"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV8027574.1"
                     /translation="MDDVSQAARGGQGGDARLAALLQREGSAYDVAGVRQLLAGVRAA
                     PPGHDPEAWMTLVVAVPSPALRRELSSLAAEADAASAAALSPAERLARLRAELARRGV
                     HGFLVPRADEHQNEYVPSHAQRLAWLTGFTGSAGLAVVLPGTAAIFVDGRYTLQAQAE
                     VDDTLYVRRHLTEQPASEWVAATLKPGETLAYDPHLHTAGEVERFRAAAEKAGGRLAP
                     LPDNPLDAVWADRPPPPLAPVMRQDLRFAGESAEQKRQALAMGLAAEQVDAVVLTAPD
                     SIAWLLNIRGGDVPHTPLPLSFALLHADAGVDLFIDRRKLVPGLIEYLGNSVRIASPD
                     SFGEALEQLGAARKRVQVDVASASAWVVHQLAEASAQIHRAADPCQLPKARKNPTELA
                     GTRAAHRRDGAALTQFLAWIAETAPKGGLGEIAASDRLEAFRRHGEHFRDLSFPTISG
                     AGANGAIVHYRASPRSERRLEPGTLYLVDSGAQYLDGTTDVTRTVAIGMPSAEMRDRF
                     TRVLKGHIALATCRFPHGTTGSQLDALARRALWEVGLDYDHGTGHGVGSYLAVHEGPQ
                     RISKLPNTQALLPGMIVSDEPGYYKAGAYGIRIENLVVVTPVAATDGERELLGFETLT
                     LAPIDRSLIDTALLDAAEIAWLDAYHARVAAELAPLVDAATRQWLTAATRPIRAA"
     gene            4058..4285
                     /locus_tag="VGP50_09100"
     CDS             4058..4285
                     /locus_tag="VGP50_09100"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEV8027575.1"
                     /translation="MLNSIGNLLHRTADAMARRRELRRAYAELSALDDRSLADIGISR
                     SEIAYALTHSTRAHTPATSATPVAAELRHAA"
     gene            4416..>4711
                     /locus_tag="VGP50_09105"
     CDS             4416..>4711
                     /locus_tag="VGP50_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV8027576.1"
                     /translation="MTEEAHRAGAGFALAAFLGVLLNLGFVVVETTYGVVGHSVALLA
                     DAGHNLGDVLGLAAAGVAALLARRRPSHRYTYGLRSSSILAALFNAIVLLIAVGA"
BASE COUNT          789 a         1571 c         1606 g          745 t
ORIGIN      
        1 cgattcatct atgcgctggg aattccgcag gtcgggcaac agacggcgaa gctcctcgcc
       61 cgtcattacc gcagtttcgc gcgctggcag gaggcgatga cggcagcgca ggaccaggag
      121 agcgacgcct ggcgcgagct caacgacatc catggcatcg gcgaggacat ggccgccgac
      181 atcgtcggct tcttcgccga gcgccacaac cgcgcgcttc tcgacgagct gcgccgcgag
      241 gtcgatgtca ccgactatgc cagcgggccg agcgccgcca acgcgccgtt gaccggcaag
      301 accatcgtct tcaccggcac gctgaccgcg atgtcgcgct ccgaggcgaa ggcacgggcg
      361 gaagcgctcg gcgccaacgt cgcggcgagc gtatcggcca agaccgacta tctcgtcgtc
      421 ggtgccgatg ccggctcgaa ggccgagaaa gcccgcgcgc tcggcgtcac gaccatgacc
      481 gaggaagaat ggctggcgct ggcgcgcggc ggctgacctt atgccgattc gctgagcccc
      541 ctctcccctt gtgggagcgg gtcgggtggg gggtgcggcg gacgctaagc tgcgcggatc
      601 ggccgcgtta ccgccgccaa ccaccggcgt gtcgcggcgt cgaccagcgg cgcgatctcc
      661 gcggcgacac gcgcgtggta agcgtcgagc caggcgatct cggccgcgtc gagcagcgcc
      721 gcatcgacga ggccgcggtc gatcggggcg agcgtcagcg caccgactga ctccgccgct
      781 cttcattaaa ctccacaaga tgccacgccg aatagtgctg cggctttgtg ccagattcgg
      841 aagcgatcgc ctctcgaatg acgtggcgga tcgcgttgct ggcgcggcat gttgaggatt
      901 atattgattc tgcgacatcg aggcacggaa gacgtagcat cagtccggcg aaatccaagg
      961 agaagcaaag gtgggtatgg ttcgcatcgc aaccgaggcg gacgccgacg cgatagcttc
     1021 tatttatgag ccgtatgtgc gcgagacggc gatctcattc gaggaaatgc cgcccacgcc
     1081 ggtcgagatg gcgcatcggc tgaggtcgac aagagatacc tatccctacc tggtgttcga
     1141 cgatggcgac ggcgtgcttg gctacgccta tggcagcgtt caccgcgcca agcccgctta
     1201 ccgatggtcc gtagaaacta ctttgtacgt ggcccgtagc gcgcatcgca ggggaattgg
     1261 ccgggcgctc tataccgaac tcctccactt gctgacccgg caaggattcc actcggcctt
     1321 cgccggcatc gttccgccga acgagaagag cgttgggcta catgaatcca tgggctttgt
     1381 gtatctcggc acatttgtcg agataggtta caagcacggt gaattgcatc acctcggctg
     1441 gtggcgtcga acgctgaagg agggaaggcc gcagcacgag cccatcccgt tcacgtcgct
     1501 tgtgtaacag aattgggatt agacccaggg ggtgactatt cggagatcta tcatcgggaa
     1561 aattcttaag atgcacgttt ccactccaac ccaatgccta caaggtcacc gactggtgcg
     1621 catctccgag agcgttcgac ggccccgggt tcctaccgct ctgggtcaac gacggaaggc
     1681 gccaggcgtg ggcaacaccg ccgctagcaa cggaagcacc cgcgctgcct gagccgcacg
     1741 agcatcatgt ggcggatggg ggatgttggc actgactcgc tgagtacccg ctccccttgt
     1801 gggagggtcg atgtgatggg tgccgcggac gctaagctgc gcggatgggc cgcgttgccg
     1861 ccgtcagcca ctgacgtgtc gcggcgtcga ccagcggggc gagctcggcg gcgacgcgcg
     1921 cgtgataggc gtcgagccag gcgatctcgg ccgcgtcgag cagcgccgtg tcgatgaggc
     1981 tgcggtcgat gggggcgagc gtcagcgtct cgaagcccag gagctcgcgc tcgccgtccg
     2041 tcgccgcgac cggcgtcacg acgacgaggt tctcgatccg gatgccgtac gcgccggcct
     2101 tgtaatagcc tggctcgtcc gagacgatca tccccggcag cagcgcctgc gtgttcggca
     2161 gcttcgagat gcgctgcggt ccttcatgga cagcgagata gctgccgacg ccatggccgg
     2221 tgccgtgatc gtaatcgagc ccgacctccc agagggcgcg gcgcgccagc gcatcgagct
     2281 gcgagccggt cgtaccgtgg ggaaagcggc aggtggcgag cgcgatatgc cctttgagca
     2341 cgcgggtgaa gcgatcgcgc atctcggcgc tcggcatgcc gatcgccacc gtgcgcgtga
     2401 cgtcggtggt gccgtcgaga tactgcgcgc cggaatcgac gagataaagc gtgccgggct
     2461 cgagccggcg ctcgctccgg ggcgaggcgc ggtaatgcac gatcgcgcca ttggcgccgg
     2521 cgcccgagat ggtcggaaag ctcagatcgc ggaaatgctc gccgtggcgg cgaaacgcct
     2581 cgaggcggtc ggacgcggcg atttcgccga gcccgccctt gggcgccgtc tcggcgatcc
     2641 aggcgaggaa ctgcgtcagt gccgcaccgt cgcgccgatg cgccgcgcgc gtcccggcga
     2701 gctcggtcgg gttcttgcgg gccttcggca gctgacaggg atcggcggcg cgatggatct
     2761 gtgcgctcgc ctccgcgagc tgatgcacca cccacgctga ggcgctcgcg acatcgacct
     2821 gcacgcgctt gcgggcggcg cccagctgct ccagcgcctc gccgaaagag tcgggcgaag
     2881 cgatgcgcac gctgttgccc agatactcga tgagccccgg aaccagcttg cggcgatcga
     2941 tgaagagatc gacgccggca tcggcgtgga gcagcgcgaa agagagcggc agcggcgtgt
     3001 gcggcacgtc cccgccacgg atattgagaa gccacgcgat cgaatcgggc gccgtcagta
     3061 ccaccgcgtc gacctgctcc gccgcgaggc ccatggccag agcctgccgc ttctgctcgg
     3121 cgctctcgcc ggcgaaacgc agatcctgcc gcatcaccgg cgccagcggc ggcggcggcc
     3181 ggtctgccca gacggcgtcg agcggattgt caggaagcgg cgccagacgg ccgccggcct
     3241 tctcggccgc cgcgcggaag cgctcgacct cgccggccgt gtggagatgc gggtcatagg
     3301 cgagcgtctc gccgggcttc agcgttgccg cgacccattc gctcgccggc tgctcggtga
     3361 gatgacgccg aacatagagc gtgtcgtcca cttcggcctg cgcctggagc gtatagcggc
     3421 catcgacgaa gatcgcggcg gtgcccggca gcacgacggc gaggccggcc gacccggtga
     3481 agccggtgag ccaggcgagg cgctgcgcat ggctcggcac atactcgttc tggtgctcat
     3541 cggcgcgggg cacaaggaag ccatggacgc cgcgtcgcgc cagctcggcc cggagccgcg
     3601 ccagacgctc ggccggcgag agcgccgctg ccgacgctgc gtccgcctcc gccgccagcg
     3661 acgagagctc gcgccggagc gccggcgagg gcaccgcaac caccagcgtc atccaggcct
     3721 cgggatcatg ccccggcggt gcggcgcgca cccccgccag caattgacgc acgccggcga
     3781 cgtcataggc cgagccctcg cgctgcaaca gcgcggccag ccgggcatcg ccgccctgcc
     3841 cgccgcgcgc cgcctgcgac acatcgtcca ttctcgcctc cctgggccga agctaaggcg
     3901 ccatctcccg ccagacaagg gcgccgccgt cgtgaccggc gccatgcgca tgacgtaagg
     3961 ctgaattgcg gcacgagaga attaatcttc gatcaaccat gaccacatta ttgtgcatga
     4021 gcgagatcat ttcgcagttg cgttcggagt tactaagatg ctgaattcaa ttggtaatct
     4081 gctgcaccgc acggccgatg ccatggccag gcggcgcgag ttgcggcgcg cctatgccga
     4141 gctctcggcg cttgacgacc gctcgctcgc cgacatcggc atcagccgct ccgaaatcgc
     4201 ctatgccctg acgcacagca cgcgggcgca cacgcccgcg acgtccgcga cgccggttgc
     4261 cgccgagctt cgccacgccg cctgacgcgg gcgttgcccg caacgacttg cacagcgaag
     4321 ccaccgcggc aatgttgatt gccgtagagg cagtcacagg gaactgatcc cgcggactcg
     4381 cgggtcggtt ggatgcaatg ccgcatggcg aggcaatgac cgaggaagca caccgcgcgg
     4441 gcgccggctt cgcgctggcg gccttcctcg gcgtgctcct caatcttggc ttcgtcgtcg
     4501 tcgagacgac ctacggcgtc gtcggacatt cggtggcatt gctcgccgac gccggtcaca
     4561 atctcggcga cgtgctgggc ctggcggccg cgggggtagc ggctctgctg gcacggcggc
     4621 ggccgagcca tcgctacacc tatggcctgc gctcgagctc gattcttgcc gcgctcttca
     4681 atgccatcgt cctgctgatc gcggtcggcg c
//
LOCUS       DASYNS010000295        12084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1004884, whole genome shotgun sequence.
ACCESSION   DASYNS010000295 DASYNS010000000
VERSION     DASYNS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 12084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12084
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004884"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VGP50_09110"
     CDS             <1..1229
                     /locus_tag="VGP50_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8027577.1"
                     /translation="ARRMGPGRDLHGLKKDGSEFPVEIGLNPIETEDGTMVLATILDV
                     TARKRMEERFRQVVEAAPNAMVMIDRNGRIELVNVEAERLFSYARAELLAQPVEMLVP
                     ARFRSAHPGLRTAFFAAPKARRMGAGRDLYGLKKDGSEFRVEIGLNPIETDDGMMVLA
                     AIVDISERIRAEERLKEQSEELLRSNNELEQFAYIASHDLKAPLRAIKSLAEWIAEDV
                     AATGSKETHENLALLQARTERLENLLSGLLEYSRVGITKADPERVDTGRLVAEIIEYL
                     APPPAFVVECRSFMPILRAAKAPLEHVFQNLISNALKHHDRDRGTVVVSARDLGALAE
                     FTVRDDGPGIDPAFHERIFGMFQTLKPRDEVEGSGVGLAIVKKAVEKNGGNIKVESAP
                     PLRGTEFVFTWRKSAE"
     gene            1226..1657
                     /locus_tag="VGP50_09115"
     CDS             1226..1657
                     /locus_tag="VGP50_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8027578.1"
                     /translation="MKTETVTILLVEDDKVDVMAVKRAFKDLRIANPLVVAKDGVAAL
                     DLLRGTNGCEKLPPPHLIMLDLNMPRMGGIEFLQVLRSDPALSKTVVFVLTTSDAPED
                     RNAAYEKYISGYVLKMQAGESFIEAISMIEHYWKIIEFPAT"
     gene            1712..4195
                     /locus_tag="VGP50_09120"
     CDS             1712..4195
                     /locus_tag="VGP50_09120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8027579.1"
                     /translation="MEKSVRKILIVDDSSDDHRIFKRMLRQAERHSYEIVSATEAAGG
                     LAAVRLHNFDCILLDINLPGQSGLDLLTELAQQYGFGLCAVVVITGGGNESVAVEAMQ
                     RGAQDYLSKVGLGAGTLERAIDRASEKRILQAQLECSLQVSTEMNRLLQLEIDERKKA
                     ELGAAAAKEQAERANEAKTAFLTNMSHELRTPMNGILGMTSLLLETALAEEQRQFATA
                     IRHSANALLTLLNDILDVSKLEAGQLELETIDFDLEELIDDALATVSVTAADKDVELC
                     ALVEESPLHQYHGDPTRLRQVMLNLIGNAVKFTKAGSVTVRAHVVSPDTSGTPGPAHL
                     RIDVTDTGIGISNDGLSRLFQKFHQADSSITRRFGGSGLGLAISRELAELMGGRIEVA
                     STLGEGSMFSLVIGLPQGAPLASPPPWTKRLANRRILIVDDLDMARRALCRRLQRMGI
                     EVTEAHDGPSALAALQRASAAGHGFDAAFIDQVMPGMSGEEVAQEIDEIPALASVKIV
                     LMSLLGIAAKTGGASRVRIDAVMTKPQSRKAVAACLAHLLFPGETLIAKENTSFLADQ
                     NVQDRHGNVQRILLAEDNVINQQVASGILRRAGYTVDVVDDGVAAVAAAKRATYDIIL
                     MDLQMPEMGGVEATDIIRKLAGFQYTPIIAMTAYAMQGTREECVRAGMDDYVAKPFDP
                     RGFLAVVRRWASVTDARAAADTLQAAPESSDPLLIDENHIAALRDTMVASDFDALMAG
                     VPARLQDRVGRLQSVFATGDLAALEREAHKLISGAGNVGATALSMLAREIETSASTQD
                     RSTVDSLMAAIGGKMSATLAALSARDLAA"
     gene            4538..7216
                     /locus_tag="VGP50_09125"
     CDS             4538..7216
                     /locus_tag="VGP50_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8027580.1"
                     /translation="MIALFGRAADIPLLYSLHQGWASMKPNTAAGFVAAGMALYLSSL
                     DRRDRFRRAGAACAAFAALIGLLTLVEYAFAVDLGIGQILFAVSGGSHAARMSPASAS
                     CLVIYGAALLLGTSARLRLRLVSENLSILVLLAAYVAILGYAYDVSVLYAVGPYNSVA
                     LPTAILFVVLATGLELADAERGLMRVLTSPYAGGVAARRLLPLALLAPTAIGWLRLEG
                     QHLGWYDTEFGVALMVVANATIFAAVILGTAGPLDNADQARRQEEALRKSEERYRLIA
                     DNVTDVIVQLDLDGIRRYVSPSARRALGYAPEELVGQPIFAILNPEHEARMRQVLEAM
                     RAGMDYATLSIKIRHKDGGERWVEAQLHLVRDAITDEPRDIVVTLRDVSSRKALEEQL
                     SREKERAEAANRAKSEFLSRMSHELRTPMNGVIGFANILLGGALDPQQRRHVVMLKEA
                     GHALLAIINDILDVAKIEAGKLELFSKPLNPAVIAERCLALVQPQAAAKGITLTCDRA
                     PDLPAWVAGDASRLQQILLNLVGNAAKFTERGGVTVTVRRAPGGTGIRFAVTDTGPGI
                     SADSQQLLFKEFSQVGQGAEHRYGGTGLGLAISKRLTEAMGGSIGVESTPGVGSTFWF
                     VVDLPPTAAPAVVEPEPEIRSATRPARILVAEDVPMNQIVVGTLLRGAGHDVTFANNG
                     AEAVRAIQDADYDLVLMDMEMPEMDGIEATRAIRALDQRGRDVPIIALSANAMSEEVE
                     RCLVAGMNGHLAKPVAPEELERTVARWMASTPAPARARKHEGVFEGATVDALEKRLGR
                     DRFASLVRMFRDQLDNAMRVMATSDHAAIAHEAHAMVSSAGNLGMTELKNSSLALMTA
                     IKKKHAAEVPALVARMEAAAARAYAAIDARQAAAIA"
     gene            complement(7282..7680)
                     /gene="acpS"
                     /locus_tag="VGP50_09130"
     CDS             complement(7282..7680)
                     /gene="acpS"
                     /locus_tag="VGP50_09130"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV8027581.1"
                     /translation="MILGIGSDLIDIRRIERTIERYGDRFLSRIFTAAERERSDRRAH
                     RVASYAKRYAAKEACSKALGTGFRRGVYWRDLGVVNLASGRPTLVLTGGALARLEAMT
                     PAGMIARIDLTLTDEPPIAQAFVIITAEAG"
     gene            complement(7946..8692)
                     /locus_tag="VGP50_09135"
     CDS             complement(7946..8692)
                     /locus_tag="VGP50_09135"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEV8027582.1"
                     /translation="MAPQRQLRLGVNIDHVATVRNARGGRHPDPLRAALLAADAGADG
                     ITAHLREDRRHIVDDDIARLVREIGLPLNLEMAATAEMLAIALRHKPHAACLVPEKRT
                     EITTEGGLDVVGGHNHLAPFVRALGGGGIRVSLFIDPEPRQLDMAKAVGAPVVELHTG
                     AYCDAVGDARARELERLVTAAAYAEAIGLECHAGHGLGYDTVGPVAAISTIVELNIGH
                     FLVGEAIFIGLDAAIRRMRALMNEARGRAT"
     gene            complement(8697..10832)
                     /locus_tag="VGP50_09140"
     CDS             complement(8697..10832)
                     /locus_tag="VGP50_09140"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV8027583.1"
                     /translation="MMRQFELVERVRAYDPSADEDALNRAYVFSMKAHGSQLRASGDP
                     YFSHPVEVAGILARMKLDSASIVTGLLHDTVEDTVATLEDIERLFGPDIARLVDGVTK
                     LSRLELQSDQTKQAENFRKLVLAMSEDIRVLLVKLADRLHNMRTLHFIKDEEKRRRIA
                     RETMEIYAPLAERIGMHDMKDELEDLAFAELYADARQSIMARLAFLRQKGGDLVERII
                     EELNETLREAGVTAEVSGREKTAYSVWRKMQQKNVGFEQLSDIMAFRVVVDTVEQCYQ
                     ALGVIHSRYPVVPGRFKDYISTPKPNNYSSLHTGVIGPERQRIEMQIRTHEMHEVAEL
                     GVAAHWVYKQGAPKTDGRQYRWLRELLDILEHASDPQEFLEHTKLEMFQDQVFCFTPK
                     GDLIALPRGATPVDFAYAVHSVIGDTCVGAKVNGRLAPLRTQLSNGDQVDIITSKAQT
                     PSPTWERFVVTGKARARIRRFVRTQQRQQYLDLGKAILQKAFRHEGHDFQEKALEGAL
                     KSFNAAGVEDLYVAVGEGLATGREVVHAIHPPRPDAKPAKVVPLSSARGKKVSENAVP
                     IRGLIPGMALHFAGCCHPLPGDRIVGIVTTGKGVTIHTIDCDALANFTETPERWLDVA
                     WNSKGDDAAHVGRISVTLGNEPGSLGSLTTVIGKHHGNITNLKITNRSVDFFEILVDI
                     EVSDLKHLTNIIAALRATPVINSVERARS"
     gene            complement(10915..11388)
                     /gene="rpoZ"
                     /locus_tag="VGP50_09145"
     CDS             complement(10915..11388)
                     /gene="rpoZ"
                     /locus_tag="VGP50_09145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV8027584.1"
                     /translation="MARVTVEDCVLKVPNRFELVLLAAQRARDVTAGAQITLERDNDK
                     NPVVALREIADETISLDNLQNALIKGMQKHVEIDEPEEDQEIEIGAQQWPTDLAPAAG
                     TEEEEGNLDEELNEDILFVAEDDAVGLTDDVNPDVNPDPDLAEGATESPAPKRPG"
     gene            complement(11514..12008)
                     /gene="folK"
                     /locus_tag="VGP50_09150"
     CDS             complement(11514..12008)
                     /gene="folK"
                     /locus_tag="VGP50_09150"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV8027585.1"
                     /translation="MILLGLGANLPSATHGPPPATLAAAIDALAAEGVVVERRSSWYR
                     SAPLPAADQPWFVNGVIAVTTRLAPAELLALLHRTEQRFGRHRRHRNEPRVLDLDLLD
                     YQGRVESGASPVALPHPRLHERAFVLLPLRDVAPDWRHPVSGMTIDALIAALPPGQQI
                     ERVD"
BASE COUNT         2109 a         3891 c         4030 g         2054 t
ORIGIN      
        1 aggcgcgccg gatgggtccg ggtcgcgatc tccatggcct caagaaggac ggcagcgaat
       61 tccccgtcga gatcgggctc aacccgatcg agaccgaaga cggaacgatg gtcctagcga
      121 cgatcctcga cgtgaccgcc cgcaagcgga tggaagagcg tttccggcaa gtggtggaag
      181 cggcacccaa cgcgatggtg atgatcgacc gcaatggccg gatcgagctg gtgaatgtcg
      241 aggcggagcg gctcttcagc tacgcgcggg cggagcttct ggcgcagccg gtggagatgc
      301 tcgtgccggc gcgcttccgc tcggctcatc ccgggttgcg aacggcgttc ttcgccgcac
      361 ccaaggcgcg ccggatgggc gcgggacgcg atctctacgg cctcaagaag gacggcagcg
      421 aatttcgcgt cgagatcggg ctcaacccga tcgagaccga cgacggcatg atggtcctcg
      481 cggcgatcgt cgacatcagc gagcgcatcc gcgcggagga gcgactcaag gagcaatcgg
      541 aggagctcct gcgatccaac aatgagctcg agcagttcgc ctacatcgcc tcgcatgatc
      601 tgaaggcgcc gttgcgcgcc atcaaaagcc ttgccgaatg gattgccgaa gacgtcgccg
      661 ccaccggctc gaaagagacc cacgagaatc ttgcgctgct ccaggcacgg acggaacgcc
      721 tcgagaattt actgtcgggt ttgctggaat attcccgtgt cgggatcacc aaggccgacc
      781 cggaaagggt cgataccgga cgcctcgtcg ccgagatcat cgaatacctg gcgccgccgc
      841 cggccttcgt ggtcgagtgc cgcagcttca tgccgatttt gcgtgccgcc aaggcgccgc
      901 tcgaacacgt ctttcagaac ctcatcagca acgccctgaa gcatcacgat cgcgaccgcg
      961 gcacggtcgt cgtctcggcg cgggatttgg gagcgctcgc ggaatttacc gttcgggatg
     1021 acgggccggg catcgatccg gcgtttcacg agcggatctt cggcatgttt cagaccctga
     1081 agccgcgcga cgaggtggag gggagcggcg tcggcctcgc catcgtcaag aaagcggtcg
     1141 agaagaacgg cggtaacatc aaggtcgaaa gcgctccccc gctccgcggc actgaatttg
     1201 ttttcacctg gaggaaatca gccgaatgaa gacggaaacg gtcacgatcc tgctcgtcga
     1261 agacgacaag gtcgacgtca tggcggtgaa acgcgctttc aaggatttgc ggatcgccaa
     1321 cccgctggtg gtcgcgaaag acggcgtcgc cgccctggat ctgctccgtg gcacgaatgg
     1381 ctgcgagaaa ctgccgcccc ctcatctcat catgctcgac ctcaatatgc cgcgcatggg
     1441 tggaatcgag tttctccagg ttttgagaag cgatcccgcg ctcagcaaga ccgtcgtctt
     1501 cgtgctgacg acgtcggacg caccggagga tcgcaacgcc gcgtacgaaa aatacatctc
     1561 gggctatgtg ctcaaaatgc aggccggcga gtcgttcatt gaagccatct ccatgatcga
     1621 gcactactgg aagatcatcg aattcccggc cacgtaacgc ctcgcgggca gatacgaata
     1681 tggcgatcac agcggctttc gaggcatgtt gatggaaaag tcggtccgca agatactcat
     1741 cgtcgacgac tcttccgatg accaccgcat cttcaagaga atgctcaggc aggcagagcg
     1801 ccacagctac gagatcgtct ccgccaccga agccgcgggc ggattggcag cggtgcgctt
     1861 gcacaacttc gactgtatcc tgctcgacat caatctgccg ggccagagcg gcctcgatct
     1921 gctgaccgag ctcgcgcagc aatatggctt tgggttgtgc gccgtcgtgg tcatcaccgg
     1981 cggcggaaac gaaagcgtcg cagttgaagc aatgcagcgc ggcgcccagg attatctcag
     2041 caaggtcggc ctcggcgccg ggacgttgga gcgcgccatc gaccgcgcca gcgagaagcg
     2101 gatactccag gcccagctcg aatgcagcct tcaggtctcg accgagatga accggctgct
     2161 tcagctcgag atcgatgagc gcaagaaggc ggagcttggc gccgccgcgg ccaaggagca
     2221 ggccgaacgg gcgaacgagg ccaagaccgc cttcctcacc aacatgagtc acgagctccg
     2281 aacgccgatg aacggcattc tgggaatgac cagcctgctt ctggagaccg cgctcgccga
     2341 ggagcaacgg caattcgcca ccgccatccg ccattcggcc aatgccctgc tgacactgct
     2401 gaacgatatt ctcgacgtct cgaagctcga ggccgggcag ctcgagctcg agaccatcga
     2461 cttcgacctc gaggagctga tcgacgacgc cttggcaacg gtgtccgtca cggccgccga
     2521 caaggacgtc gagctctgcg ccttggtcga ggagagtccc ctgcatcaat atcatggcga
     2581 tcccacgcgg cttcgccagg tcatgctcaa cttgatcggc aacgccgtca aattcaccaa
     2641 ggccggctcc gtcacggtgc gcgcgcacgt ggtttcgccg gataccagcg ggacgcccgg
     2701 tccggcgcac ctgcgcatcg acgtgaccga taccgggatt ggcatctcga atgatggctt
     2761 gtcgcggctc tttcaaaaat tccatcaagc ggacagctcc atcacgcggc gctttggcgg
     2821 ctcgggtctc ggccttgcca tctcgcgcga gctcgccgag ctgatggggg ggcggatcga
     2881 ggtcgctagc acgctgggcg agggctcgat gttcagcctc gtgatcggat tgccgcaagg
     2941 ggcgccgctg gcttcgccac cgccatggac aaaacgcctc gccaaccgcc gcattctgat
     3001 cgtcgacgac ctcgacatgg cgcgccgcgc tttgtgccgt cggctgcagc gcatgggcat
     3061 cgaggtgacg gaagcccatg atgggccttc ggccttggcg gcgttgcaac gcgcaagcgc
     3121 ggccggccat ggcttcgatg cggcgtttat cgatcaggtc atgccgggca tgtcgggaga
     3181 agaggtcgcg caggagatcg acgagattcc cgccttggcg agcgtgaaaa tcgtcttgat
     3241 gagcctgttg gggattgccg cgaaaacggg tggtgcaagc cgggtccgga tcgacgctgt
     3301 catgaccaag ccgcaaagcc gcaaagccgt ggccgcgtgt ttggcgcatc tgctgtttcc
     3361 cggcgagacc ctcattgcga aggaaaacac gagcttcctc gcggatcaga acgtccaaga
     3421 ccggcacgga aacgtgcagc ggatcctgct tgccgaagac aacgtcatca atcagcaagt
     3481 cgcctccggc attctgaggc gtgccggcta caccgtcgac gtggtcgatg acggcgtggc
     3541 tgccgtcgcc gcggccaagc gtgcgacata cgacatcatc ctgatggatc tccagatgcc
     3601 ggaaatgggc ggcgtcgagg cgaccgatat catccggaaa ctcgctggct tccagtacac
     3661 gccgatcatc gccatgaccg cgtacgccat gcagggaacg cgcgaggaat gcgtccgggc
     3721 cgggatggac gactacgtcg caaagccctt cgacccgcgc ggattccttg cggtggtgcg
     3781 gcgatgggcg agcgtcacgg atgcgcgcgc ggcggccgac acgctgcaag cggctccaga
     3841 gtcttcggac ccgctgctca tcgatgagaa ccacatcgcg gcgcttcgcg acacgatggt
     3901 agcgagcgac ttcgacgccc tgatggccgg cgtgccggca cgtctgcagg accgcgtcgg
     3961 tcggttgcag agcgtctttg cgacgggtga tctggcggcg ctcgagcgcg aggcgcacaa
     4021 gctgatcagc ggcgccggca acgtcggcgc gacggcactc tcgatgctcg cccgcgaaat
     4081 cgagacgagt gcctcgacac aagatcgcag cacggtcgac agtctcatgg ccgcgatcgg
     4141 cggcaagatg tcggccacgc tcgccgccct gagcgccagg gaccttgcgg cctagccccc
     4201 ggagttgccc ttctaattcg tcatggccgc tatcagccaa caagggtccg atctggtcat
     4261 ctggaactga atttcgttac gggccgccgg cacaagacat agcgccgcga ggcccgacgc
     4321 ccccacgctc aagtgtttgt taatcgctgg cagcttaggc tcacagactc ataaaagtcg
     4381 tgtaccaggt cgtcggcccg cggcaggaga gacttctgcc agcggttcct cagctctgcc
     4441 gcgctcgaaa gaccaggagc cgaccacgaa aacagatttt gcggcagcag ggcggagagc
     4501 agcgcgggcg gcgggcgcgg ccgtcagcat ctccggcttg atcgccctct tcggccgggc
     4561 ggccgatatc ccgcttctct actcgctcca tcagggctgg gcgtcgatga agccgaatac
     4621 ggccgcaggc ttcgtcgcgg cgggaatggc gctctacttg tcgagcttgg atcggcgcga
     4681 tcgcttccgc cgggccggcg cggcctgcgc cgcctttgcg gcgctcatcg ggctgcttac
     4741 gctggtcgaa tacgcgtttg ccgtcgacct tggcatcggt cagattctct tcgccgtttc
     4801 cgggggcagc catgccgcgc ggatgtcgcc ggcaagcgcg tcatgcctcg tcatctatgg
     4861 cgcagccctc ctcctcggca cttccgcccg gctccgcctt cgcctcgtat cggaaaacct
     4921 gagcatcctc gtgctgttgg ctgcctatgt cgccatcctc ggctatgcct acgacgtctc
     4981 tgtgctctac gccgtcggcc cctacaattc cgtcgcgttg ccgaccgcca tcctgttcgt
     5041 cgtgctcgcc accggcctgg agcttgcgga cgcggagcgc ggcctgatgc gcgtgttgac
     5101 gagcccctat gccggaggtg tcgcggcgcg ccggctgctg ccgctggccc ttctggcccc
     5161 gacggcgatc ggttggctgc ggctcgaggg gcagcacctt ggctggtacg ataccgagtt
     5221 tggcgtggcg ctgatggtcg tcgcaaacgc caccatcttt gcagcggtga ttctggggac
     5281 cgcgggcccc ctcgacaacg ccgaccaggc gcgccgccag gaggaagcgc tgcgcaagag
     5341 cgaggagcga taccggctga tcgccgacaa cgtaaccgat gtcatcgtgc agctcgatct
     5401 cgatgggatt cgccgctacg tgtccccctc ggcacgccgc gcgctgggct acgcgcccga
     5461 agagctggtc gggcaaccca tcttcgcgat cctcaatccg gagcatgagg cccgcatgcg
     5521 ccaggtgctg gaggcaatgc gcgccggcat ggattacgcg accctctcga tcaagatccg
     5581 gcacaaggac ggcggcgagc ggtgggtcga ggcccagctc catctggttc gcgacgccat
     5641 caccgacgag ccgcgggaca tcgtggtcac gctacgcgac gtttcgtcgc gcaaggcgct
     5701 cgaggaacag ctgagccggg agaaggagcg cgccgaggcc gccaatcgcg ccaagtccga
     5761 attcctgtcg cgcatgagcc acgagctgcg gacgccgatg aacggcgtca tcggctttgc
     5821 caatatcctg ctcggcggcg cgctcgaccc gcagcagcgg cgccatgtgg tcatgctgaa
     5881 ggaagcggga cacgcgctgc ttgccatcat caacgacatt ctcgacgtcg ccaagatcga
     5941 ggcggggaag ctcgagctct tctcgaagcc gctcaatccc gctgtcatcg ccgagcggtg
     6001 cctcgcgctc gtgcagcccc aagcggcggc caagggcatc acgctgacct gcgaccgcgc
     6061 gcccgacctt ccggcctggg tcgccggcga cgcctcgcga ttgcagcaga ttctcctgaa
     6121 cctggtcggc aatgccgcga aattcaccga gcgcggcggc gtgacggtga cggtgcggcg
     6181 ggcgcccggc ggcacgggaa ttcgcttcgc cgtgaccgac accggaccag gcatctccgc
     6241 cgactctcag cagctcttgt tcaaagagtt ctcgcaggtc gggcaaggcg ccgagcaccg
     6301 ttatggcggt accggcctgg gtctcgccat cagcaaacgc ctgacggagg cgatgggcgg
     6361 atcgatcggc gtcgaaagca cgccgggcgt cggcagcacc ttctggttcg tggtcgacct
     6421 gccgccgacg gcggcgcccg cggtggtcga gcccgagccc gaaattcgct cagccacgcg
     6481 acccgccagg attctcgtcg cggaggacgt gccgatgaat caaatcgtag tcggcacatt
     6541 gctacgcggc gcgggccacg acgtcacctt cgcgaacaat ggcgccgagg cggtccgcgc
     6601 gatccaggat gcggactacg atctcgtctt gatggacatg gagatgccgg agatggacgg
     6661 catcgaggcg acgcgcgcca tccgtgctct cgaccagcgc ggacgcgacg tcccgatcat
     6721 cgcactcagc gccaatgcga tgtccgaaga ggtcgagcgc tgcctggtgg ccgggatgaa
     6781 cggtcatctc gcgaagcctg tcgctccgga ggagctcgaa aggacggtag cgcgctggat
     6841 ggcgagcacg ccggcgccgg cacgggcgcg aaagcacgag ggtgtgttcg agggtgccac
     6901 cgtcgacgcg ctcgagaagc ggctcggccg tgatcgattc gcttcgctcg ttcggatgtt
     6961 tcgcgatcag ctcgacaacg ccatgcgcgt gatggccacg tccgaccacg cggccatcgc
     7021 gcatgaggcg catgcgatgg tctcgtccgc cggcaatctt gggatgaccg agctgaagaa
     7081 ttccagcctg gcgctgatga cggcgatcaa gaagaaacac gcggcggagg tcccggcgct
     7141 cgtcgctcgc atggaagcgg cagcggcgcg agcgtacgcc gcgatcgacg cgcggcaggc
     7201 ggcggccatc gcatagccag cgattcactc ctcagccgcc accttttatc ggttttgacc
     7261 ctaacccttc tgcgcgatgc gtcaccccgc ctcggcggtg atgatgacga aggcctgggc
     7321 gatcggcggc tcgtcggtga gggtcagatc gatccgcgcg atcatgccgg cgggcgtcat
     7381 cgcctcgagc cgggcaagcg cgcccccggt caggacgagg gtcggacgcc cggaggcgag
     7441 attgacgacg cccagatccc gccagtagac gccgcgccga aagccggtgc cgagcgcctt
     7501 cgagcaagcc tccttggcgg cgtagcgctt cgcatagctc gccacgcgat gggcgcggcg
     7561 atccgagcgc tcgcgctccg ccgccgtgaa gatccggctg agaaaacggt cgccgtagcg
     7621 ctcgatggtg cgctcgatac ggcggatgtc gatgaggtcg ctgccgatgc cgaggatcat
     7681 gcgcggttcc tgcaacagcg agcagcgtcg ccccccaccc taccctcccc ctcaaagggc
     7741 agggcaatcg catgtgctgg cgagacgcca ataggaaaga ttgaccacgg aggcacggag
     7801 gacacggaga agcgaaggat ggcgcgcttc gcgcgccgag cccttcttct ccgtgtcctc
     7861 cgtgcctccg tggtgaaccc aatatgcgat agccctgtct caagggggca ggggcaagaa
     7921 ggagacgcaa gggtccggtg cagcgtcacg ttgcgcgtcc acgggcctcg ttcatcagcg
     7981 cgcgcatgcg gcggatcgca gcgtcgaggc cgatgaatat cgcttcgccg acgaggaagt
     8041 gaccgatgtt gagctcgacg atcgtggaaa tggcggcgac aggcccgacc gtatcgtagc
     8101 ccagcccatg tccggcatgg cactcgagac cgatggcttc ggcataggcc gccgcggtca
     8161 cgaggcgctc gagctcgcgc gcgcgggcgt cgcccacggc gtcgcaatag gcgccggtgt
     8221 gcagctcgac gacgggggcc ccgaccgcct tcgccatgtc gagctggcgc ggctcgggat
     8281 cgatgaagag agagacgcgg atcccgccgc cgccgagcgc gcgcacgaag ggcgcgaggt
     8341 gattgtggcc gccgacgaca tcgagcccgc cctcggtggt gatctcggtg cgcttttccg
     8401 gcaccagaca ggcggcgtgc ggcttgtggc ggagcgcgat cgccagcatc tccgcggtcg
     8461 cggccatctc gaggttgagc ggcagcccga tctcccggac gaggcgcgcg atgtcgtcgt
     8521 cgacgatatg acggcgatcc tcgcgcagat gcgcggtaat gccgtccgcg ccggcatcgg
     8581 cggcgagaag cgccgcgcgc aatggatcgg gatgccggcc gccgcgggcg ttacggacgg
     8641 tcgccacatg gtcgatgttg acgccgaggc gaagctgtcg ttgaggcgcg atcacgtcag
     8701 ctgcgcgccc gctcgaccga gttgatcacc ggcgtcgcgc gcagcgcggc gatgatattg
     8761 gtcagatgct tcaagtcgct cacctcgata tcgacgagga tctcgaaaaa atcgacagag
     8821 cggttggtga tcttgagatt ggtgatgttg ccatggtgct tgccgatgac ggtcgtcagg
     8881 ctgccgaggc tgccgggctc gttgccgagg gtgacgctga tgcgcccgac atgcgcggcg
     8941 tcgtcgccct tgctgttcca ggcgacgtcg agccagcgtt cgggcgtctc ggtgaagttg
     9001 gcgagcgcat cgcagtcgat ggtgtggatg gtgacgccct tgccggtggt gacgatgccg
     9061 acgatgcggt cgccgggcaa agggtggcag cagccggcga agtgcagcgc catgcccggg
     9121 atgaggccgc ggatcggcac ggcattctcc gagaccttct tgccgcgcgc gctggagagc
     9181 ggcaccacct tggcgggctt ggcatcgggc cgcggcggat ggatcgcgtg caccacttcg
     9241 cgccccgtgg cgagcccctc gcccaccgcg acatagagat cctcgacgcc ggccgcgttg
     9301 aacgacttga gcgcgccctc gagcgctttc tcctggaagt cgtggccctc gtggcggaat
     9361 gccttctgca ggatggcctt gccgagatcg agatattgct gccgctgctg ggtgcggacg
     9421 aagcggcgga tccgcgcgcg cgccttgccg gtgacgacga agcgctccca ggtcggcgac
     9481 ggcgtctgcg ccttcgacgt gatgatatcg acctggtcgc cgttggaaag ctgcgtgcgc
     9541 aacggcgcga gtcggccatt gaccttggcg ccgacgcagg tatcgccgat cacggaatgg
     9601 acggcatagg cgaaatcgac cggcgtcgcg ccccgcggca gcgcgatcag atcgcccttg
     9661 ggcgtgaagc agaacacctg gtcctggaac atctcgagct tggtgtgctc gaggaactcc
     9721 tgcgggtccg aggcatgctc gaggatgtcc agaagctcgc gcagccagcg atactgccgg
     9781 ccatcggttt tgggcgcccc ttgcttgtag acccaatgcg cggcgacacc gagctcggcc
     9841 acctcgtgca tctcgtgggt gcggatctgc atctcgatgc gctggcgctc cgggccgatg
     9901 acgccggtat ggagcgagct gtagttgttg ggtttcggcg tcgagatgta atccttgaag
     9961 cggccgggca ccacgggata gcggctgtgg atcacgccca gcgcctggta gcactgctcg
    10021 acggtgtcga cgacgacgcg gaacgccatg atgtcggaga gctgctcgaa gccgacattc
    10081 ttctgctgca tcttgcgcca gacggaatag gcggtcttct cgcgccccga tacctcggcg
    10141 gtgaccccgg cctcgcgcag cgtctcgttg agctcctcga tgatgcgctc gaccaggtct
    10201 ccgcccttct gccgcaggaa ggcgagccgc gccatgatgc tctggcgtgc atcggcatag
    10261 agctcggcaa aggccaggtc ctcgagctcg tccttcatgt catgcatgcc gatgcgctcg
    10321 gcgagcggcg catagatctc catggtctcg cgcgcgatgc gccggcgctt ctcctcgtcc
    10381 ttgatgaagt gcagcgtgcg catgttgtgg aggcgatcgg caagcttcac caggaggacg
    10441 cggatgtcct ccgacatggc gagcacgagc ttgcggaagt tctcggcctg cttcgtctgg
    10501 tccgattgca gctcgagccg cgacagcttg gtaacgccgt cgaccagcct ggcgatgtcg
    10561 ggtccgaaca gccgctcgat gtcctcgagc gtcgccaccg tgtcctcgac ggtgtcatgc
    10621 aggagaccgg tgacgatcga ggccgaatcg agcttcatcc gggcaaggat gccggcgacc
    10681 tcgacgggat gggagaagta agggtcgccc gaagcgcgca gctgcgagcc atgcgccttc
    10741 atcgagaaga cgtaagcccg gttgagcgcg tcctcgtcgg ccgaaggatc gtaagccctg
    10801 acgcgctcga ccagctcgaa ttggcgcatc atgacgggtc accccgccct gcgcgctgac
    10861 gcgcgcagca gaatttcggc ggcgcgatcc ctcgaagccg ggcgccgccc ggcatcaccc
    10921 cggtcgcttg ggcgccggac tctcggtcgc cccttcggcc aggtccgggt ccgggttcac
    10981 gtccgggttc acgtcgtcgg tgaggcccac ggcatcgtcc tcggcgacga acaggatgtc
    11041 ctcgttgagc tcctcgtcga ggttgccctc ttcctcctcg gtgcccgcgg ccggcgcgag
    11101 atcggtcggc cattgctgcg caccgatctc gatctcctgg tcctcctcgg gctcatcgat
    11161 ctcgacgtgc ttctgcatgc ccttgatgag ggcattctgc aggttgtcga gcgagatcgt
    11221 ctcgtcggcg atctcgcgca gggccacgac gggattcttg tcgttgtcgc gctcgagggt
    11281 gatctgggcg ccggcggtga cgtcgcgcgc acgctgcgcc gccaggagca cgagctcaaa
    11341 acgattcggt accttgagga cgcaatcttc gacggttacg cgagccatgc tgacactttc
    11401 tccggatgag acttagtctt tgactatata aggacttgcg gggcggccgc aatatccgtc
    11461 cgattctccg ccgccccgtc cttaatgatg cttaagcggc gccgcgcgcc tgctcagtcc
    11521 acgcgctcga tctgctgccc cggcggcagg gcggcgatca gcgcatcgat ggtcatgccc
    11581 gagaccggat gacgccaatc gggcgccacg tcgcgcaacg gcagcaagac aaaagcccgc
    11641 tcgtggagtc gcggatgagg cagcgccacg ggcgacgcgc cgctttcgac gcgaccctga
    11701 tagtcgagaa gatcgaggtc gagcacgcgc ggctcattgc ggtggcggcg gtgccggccg
    11761 aagcgttgct cggtccgatg cagcagcgcc aagagctcgg ccggtgccaa tcgcgtcgtg
    11821 acggcgatga cgccgttgac gaaccaaggc tgatcggccg ccggcagcgg cgccgagcgg
    11881 taccaggacg agcgccgctc gacgacgacg ccctccgccg cgagggcgtc gatggccgca
    11941 gccagcgtcg cgggtggcgg gccatgcgtc gcgctgggca ggttggcacc caggcccagc
    12001 aggatcatga aacggtcatg gtggcgcttg acggcttcgc gacattacgg acaaatgcta
    12061 aggaattgtg aagcacggct ttgc
//
LOCUS       DASYNS010000296         3804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2721757, whole genome shotgun sequence.
ACCESSION   DASYNS010000296 DASYNS010000000
VERSION     DASYNS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721757"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /gene="zwf"
                     /locus_tag="VGP50_09155"
     CDS             complement(<1..1432)
                     /gene="zwf"
                     /locus_tag="VGP50_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV8027586.1"
                     /translation="MEAVTLEKDDAAVAPCGAVPAPPCAMVIFGAAGDLTKRLLAPAL
                     YDLVLAGRLAPEFRLIGVATNPLTTADWKQSLTDMMHEFVGQSDAEFHADRLDEKAWR
                     WLTDRMTYVAGDLSDPQMYRRLGQLLSELDKTAGTAGNYLFYLAVPDRLFGEAVKRLG
                     EAGLVEETENHWRRVVIEKPFGHDLASARALNAEILKTLKEHQIYRIDHFLGKETVQN
                     VMALRFANGLFEPIWNRDHIDHIEITASETVGVERRGKFYESTGALKDMVPNHMFQLL
                     AMTAMEPPASFEAEAVRAKKAEVIEAIHPMSEADALRDAVRGQYGPGAVLGRRVNGYR
                     QEPDVAPNSNTETFAALKLNIDNWRWAGVPFYIRTGKYMSRRRTEIAIRFHQAPYSIF
                     RGTDVERMHPNWMILRIQPDEGIGLEFAAKRPGPTVTLGTVSMDFAYKSYFKVVPSTG
                     YETLIYDCMIGDQTLFQRADNIEAGWR"
     gene            complement(1420..3480)
                     /gene="tkt"
                     /locus_tag="VGP50_09160"
     CDS             complement(1420..3480)
                     /gene="tkt"
                     /locus_tag="VGP50_09160"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011253225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV8027587.1"
                     /translation="MTDEQLDQLAINTIRTLSIDAVQQANSGHPGAPMALAPLVYTIW
                     NRAMRFDPQDPIWPDRDRFVLSNGHASMLLWSVLYLTETVAVNAEYERVGKPAVTLDD
                     IKRFRQIDSKAPGHPEYHLVSGVEATTGPLGQGIANSVGMAMARKWLAQRYNRPGFEI
                     FDYNIYVTCGDGDLMEGVGSEAASLAGHLGLDDLCWIYDNNHITLDAPTSVTFPEDVT
                     ARFLGYHWNVLRVGDANDTESIENALAVFRKTSGRPTLIILDSHIGYGAPDVVDTPAA
                     HGEPLGEKEVRLAKRAYGWPEDAKFLVPDGVLEHFAQGIGARGAAARAKWEALFAAYR
                     RQFPELAAEIEKMQRRELPEGWDRNLPRFPADAKGMAGRDASGQVLNVLAQNIPWLLG
                     GSADLASSNRTALKFAGAGDFQAATPGGRNIHFGVREHAMAAIVNGLSLSKLRAFGAT
                     FFIFSDYARPSIRLSALMELPAIWVFTHDAMGDGEDGPTHQPVEQLISLRAVPGLTMF
                     RPADANEVVEAYRYIMQLRHRPAALALSRQALPIFDRSRYASAEGVTRGAYVMADPPK
                     GSPEIIFIATGSEVAIAVGAHETLTAEGVRSRVVSMPSWDVFEAQPQSYRDSVLPPQV
                     TARIAIEQGSVIGWDRYVGAAGKVVGMKTFGGSAPLKDLLHKFGFEPEKVVGLAKEML
                     QWKR"
     gene            complement(3640..>3804)
                     /locus_tag="VGP50_09165"
     CDS             complement(3640..>3804)
                     /locus_tag="VGP50_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase
                     (decarboxylating)"
                     /protein_id="HEV8027588.1"
                     /translation="EGRWTIKAAIDEGVAAHVLSSALYERFSSRGQSDFADKLLSAMR
                     YEFGGHVEKA"
BASE COUNT          639 a         1248 c         1248 g          669 t
ORIGIN      
        1 cccgccaccc cgcctcgata ttgtcggcgc gctggaacaa ggtctggtcg ccaatcatgc
       61 aatcatagat cagcgtctcg tagcccgtgc tcggcaccac cttgaaatag ctcttgtaag
      121 cgaagtccat ggagaccgtg ccgagcgtga ccgtcgggcc cgggcgcttg gcggcgaatt
      181 cgaggccgat cccctcgtcg ggctggatgc gcaggatcat ccaattggga tgcatgcgct
      241 cgacatcggt gccgcgaaag atcgaataag gcgcctggtg aaagcggatc gcgatctcgg
      301 tccgccggcg gctcatatat ttgccggtgc ggatatagaa gggcacgccg gcccagcgcc
      361 agttgtcgat gttgagcttg agcgccgcga aggtctcggt gttcgagttc ggcgccacgt
      421 cgggctcttg gcgatacccg ttcacgcgtc ggcccagcac cgcgcccggt ccgtattggc
      481 cgcgcaccgc atccctcaag gcgtccgcct ccgacatggg atggatggct tcgatgactt
      541 cggccttctt cgcgcggacg gcttccgctt cgaaggaggc cggcggctcc atcgccgtca
      601 tcgccaggag ctggaacata tggttgggca ccatgtcctt gagcgcaccg gtgctctcgt
      661 agaacttgcc gcgccgctcg acgccgaccg tctctgacgc ggtgatctct atgtggtcga
      721 tatggtcgcg attccagatc ggctcgaaca ggccattggc gaagcgcagc gccatgacgt
      781 tctgcaccgt ctccttgccg aggaaatgat cgatgcgata gatctggtgc tccttcagag
      841 tcttcagaat ctcggcattg agcgcccggg ccgaagcgag gtcgtggccg aacggcttct
      901 cgatcaccac gcggcgccag tgattctccg tctcctcgac gagaccggct tctcccagtc
      961 ttttcaccgc ttcgccgaac aagcgatcgg ggaccgccaa atagaagaga tagttgcccg
     1021 cggtgccggc ggtcttgtcg agttcggaca ggagctggcc aagccggcga tacatctgag
     1081 gatcgctgag gtcgcccgcg acataggtca ttcggtcggt gagccagcgc caggccttct
     1141 catcgagtct gtcggcatgg aactcggcat cgctctgccc gacgaactcg tgcatcatgt
     1201 ccgtcaggct ctgcttccaa tcggccgtcg tcagcgggtt ggtggcgacg ccgatgagcc
     1261 gaaactccgg cgcgaggcgg cccgcgagca ccaggtcgta gagcgccggc gccagcaggc
     1321 gcttcgtcag gtcgccggcg gcgccgaaaa tgaccatcgc gcagggcggc gccggcacgg
     1381 cgccacaggg cgcgaccgcg gcgtcgtcct tctccaatgt caccgcttcc attgcagcat
     1441 ctccttcgca aggccgacga ccttctccgg ctcgaagccg aatttgtgca gcagatcctt
     1501 gagcggcgcc gagccgccga aggtcttcat gcccaccacc ttgcccgccg cgccgacata
     1561 gcggtcccag ccgatcaccg agccttgctc gatggcgatg cgcgccgtga cctgcggcgg
     1621 cagcacactg tcgcgatagg attgaggctg cgcctcgaag acgtcccagg atggcatcga
     1681 cacgacgcgc gagcgcacac cctcggccgt cagcgtctca tgcgcgccga ccgcgattgc
     1741 gacctcactg ccggtggcga tgaaaatgat ctcgggcgag cccttgggcg gatcggccat
     1801 gacatacgcg ccgcgcgtca cgccttcggc cgaggcgtag cggctgcgat cgaagatcgg
     1861 cagagcctgc cgcgacagcg cgagcgccgc cgggcggtgg cgcagctgca tgatatagcg
     1921 ataggcctcg accacctcat tggcatcggc gggccggaac atagtgaggc ccggaacggc
     1981 acgcagcgag atcagctgct cgaccggctg atgcgtcggt ccgtcctcgc cgtcgcccat
     2041 ggcgtcatgg gtgaagaccc agatcgccgg cagctccatc agcgccgaga ggcggatcga
     2101 cggccgggcg tagtcgctga agatgaagaa ggtcgcgccg aaagcgcgca gcttcgagag
     2161 cgacagcccg ttgacgatcg ccgccatggc gtgctcgcgg acgccgaaat gaatgttccg
     2221 cccgccaggg gtcgctgctt ggaaatcgcc ggcgccggcg aatttgagcg ccgtccggtt
     2281 ggacgacgcg agatcggccg agccgccgag gagccaggga atgttctgcg ccagcacgtt
     2341 cagcacctgc cccgacgcgt cgcgccccgc catgcccttg gcatcggccg ggaagcgcgg
     2401 caggtttcgg tcccatccct cggggagctc gcggcgctgc atcttctcga tctctgcggc
     2461 gagctccggg aattgccggc ggtaagcggc gaacagcgct tcccatttcg cccgcgcggc
     2521 ggcgccccgc gcgccgatgc cttgtgcgaa atgctcgagc acgccgtcgg gcacgaggaa
     2581 tttcgcatcc tcgggccagc cataggcgcg cttcgcgagg cgtacttctt tctcgcccag
     2641 gggctcgcca tgggccgcgg gggtgtcgac gacatcgggc gcgccgtagc cgatatggct
     2701 gtcgaggatg atgagcgtcg gccgaccgga cgtcttgcgg aagaccgcga gcgcattctc
     2761 gatgctctcg gtgtcgttgg cgtcgccgac gcgcagcacg ttccagtgat atccgaggaa
     2821 ccgcgccgtc acgtcctcgg ggaaggtgac gctcgtcggg gcgtcgaggg tgatgtggtt
     2881 gttgtcgtag atccagcaga gatcgtcgag gccgagatgc cccgcgagcg acgccgcttc
     2941 ggagcccacg ccctccatga ggtcgccatc gccgcaggtc acgtagatgt tgtagtcgaa
     3001 gatctcgaag cccggccggt tgtagcgctg cgccagccac ttccgcgcca tcgccatgcc
     3061 gacgctgttg gcgatgcctt gacccaaggg gcccgtggtg gcctcgacgc cagagaccaa
     3121 atgatattcc gggtgccccg gcgccttgct gtcgatctgg cggaagcgct tgatgtcgtc
     3181 gagcgtaacc gccggcttgc cgacccgttc atactcggca ttcaccgcca ccgtctcagt
     3241 caggtagagc accgaccaga gcagcatcga ggcatggccg ttggagagca cgaagcggtc
     3301 gcggtcgggc cagatcggat cctgcgggtc gaagcgcatc gcccggttcc agatcgtgta
     3361 gacgagcggc gccagcgcca tgggcgcgcc gggatggccc gaattcgcct gctgcaccgc
     3421 gtcgatcgac agcgtgcgga tcgtgttgat cgcgagctgg tcgagctgtt cgtcggtcat
     3481 gcctctttct cccggaaatc gaaccacgct tcgtcatccc cgcgaaagcg gacccagggc
     3541 aagtgacgtg ctgatggccc ctgggtcccc gctttcgcgg ggatgacgat gtcggagaat
     3601 cgacgattgg acccgatggg catccatcac ggccatctct caagccttct ccacatggcc
     3661 gccgaactcg tagcgcatgg ccgagagcag cttgtcggcg aaatcggact ggccgcgcga
     3721 gctgaagcgc tcatacaagg ccgatgagag gacatgggcc gcaacgcctt catcgatcgc
     3781 ggccttgatg gtccagcgtc cctc
//
LOCUS       DASYNS010000297         6578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1005248, whole genome shotgun sequence.
ACCESSION   DASYNS010000297 DASYNS010000000
VERSION     DASYNS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005248"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1144
                     /locus_tag="VGP50_09170"
     CDS             257..1144
                     /locus_tag="VGP50_09170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEV8027589.1"
                     /translation="MTVSSMTGFARADGHEDGVSWVWEVKSVNGKSLDLRFRLPPGLE
                     SLELPLRAGIARRLKRGALSLTLTLTRAGAAGGLRVNREALAQVVALAQELVAQGRAA
                     PPRADGLLALRGVLESGDEKEEEGDRERREASLLASSETALDRLVAMRLAEGARLIPL
                     LEARLADIARLVDLAENSAGARPEALKARLQELVAALLEASPSLPPERLAQEAALLVA
                     KADVREELDRLSGHIIAAQELLAEGGAIGRRLDFLCQEFNREANTLCAKSGDMALTRI
                     GLDVKAAIDQLREQAQNVE"
     gene            1153..1797
                     /gene="gmk"
                     /locus_tag="VGP50_09175"
     CDS             1153..1797
                     /gene="gmk"
                     /locus_tag="VGP50_09175"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV8027590.1"
                     /translation="MSASEITRRGILLVLSSPSGAGKTTITRELVARDPLLRISVSVT
                     TRPRRRDEVDGQHYHFIAPAQYDAMVAKGELLEHATVFGHCYGTPRAPVEAALAAGRD
                     IVTDIDWQGTQQLNGTIRADLVAVFVLPPSMAALEQRLRTRAQDSDDVVRGRMAKSAD
                     EMSHWPEYDYVFVNRDIAESVRQVQSILSAERIRRERLLGLADFVNRLRAGSQA"
     gene            1794..3221
                     /locus_tag="VGP50_09180"
     CDS             1794..3221
                     /locus_tag="VGP50_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV8027591.1"
                     /translation="MTLREDYERYDGLGLAALVRQREVSPGELLDAAIERVERRNPAI
                     NAVIDRFYDRAKAAIAAGLPSGPFTGVPYLLKDYGVYLAGTRTTGGSRLFRDAVADHD
                     SEIVLRMKRAGLVIFGKTNTSELGLSTSVEPLLFGPTRNPWQRDHSAGGSSGGAAASV
                     ASGMVPMAHATDGGGSIRIPASCCGLFGLKPTRARTPLGPDLGESWNGAGVGHAVTRS
                     VRDSAALLDATAGPDIGDPYWAPPLRGSFLAEIGADPGRLKIALATRPGTGDAVDPEC
                     VAAATAAARLCESLGHEVVEAAPEIAEPERSAAARVIATAHTSVTLELRAKALGRALR
                     PDDVEPQTWAAAEFGRQESASAYANAIRVLHRAGRVVGRFFKRYDVLLTPTMCRPPHK
                     IGVIAASNRDQRAFVEALLGTIAFTSPFNGTGNPAMSVPLHWSPDGLPIGVQFVAGFG
                     EEATLFRLAAQLEKAQPWADRRPSI"
     gene            complement(3467..4804)
                     /locus_tag="VGP50_09185"
     CDS             complement(3467..4804)
                     /locus_tag="VGP50_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEV8027592.1"
                     /translation="MRSLLLTAAALLASLLLAGCGDEASQPLSAGVGPDPKLPPPHHT
                     LIPTVNIAPAKGWAAGESPTPAPGLAVAVFADKLDHPRWLFVLPNGDVLVAETNAPDR
                     PEDDTGIFGFAAQLIMKRAGARVPSANRITLLRSDGGGTAETSSVFLEGLTSPFGMAL
                     IGDALYVADTDALLRFPYHAGDTKITAAGVKVADLPAGPINHHWTKNVIAGADGKRLY
                     VTVGSNSNVGDHGIAAEEGRAAIWEIDPRTGDHRIFASGLRNPNGLAWEAATDALWTV
                     VNERDELGSDLVPDYLTSVRDGGFYGWPYSYYGQHVDERMTPPRPDLVAQAIAPDYAL
                     GPHVAALGLTFADRAKLGIGFASGVFIGEHGSWNRRPASGYKVVFVPFAGGRPSGEPI
                     DILTGFLDGDGNARGRPVGVAIDHGGALLVADDVGNCIWRVTASATASADRPH"
     gene            complement(4842..5675)
                     /gene="rsmA"
                     /locus_tag="VGP50_09190"
     CDS             complement(4842..5675)
                     /gene="rsmA"
                     /locus_tag="VGP50_09190"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV8027593.1"
                     /translation="MAEPDRALPPLREVIRRYELGARKALGQNFLLDLNLTRRIAREA
                     APLADAGIIEIGPGPGGLTRALLLEGARHVTAIERDRRCIAALEELAAAFPGRLAVVE
                     GDALEIDPRDLVPEPRKIVANLPYNIATALLLRWLPTIGAYESLTLMFQKEVAQRLAA
                     APRSRDYGRLSVMVQWCCQVRALFDIPPQAFVPPPKVTSTLVQLLPRAAPLAPCRFRD
                     LERVVAAAFGQRRKMLRQSLRSLGVDVEALLAATGIAATARAEELGVEQFCALARAFG
                     G"
     gene            complement(6124..>6578)
                     /locus_tag="VGP50_09195"
     CDS             complement(6124..>6578)
                     /locus_tag="VGP50_09195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8027594.1"
                     /translation="LEYETDRKYDAVVIMGVIEHLPDYQRVIEKFMSLLKPGAPIFLD
                     GSAATRKYELSSFMVKYVYGGNHSFLVLHDFLDKLARTPLRVVELFNDRESYFLTFQQ
                     WARNLDHNREAVIARFGEFNYRRFRFYLWGAAYEFLSGSLDCYRMIIR"
BASE COUNT         1059 a         2185 c         2256 g         1078 t
ORIGIN      
        1 aagcgcatcg ccgccgccct caccgctctc cccccaatcc cctgccatct ccccgatccc
       61 cgatcccttt caatggcgga aattctaaga tcgaaaggag cctacgttaa cgcctatgca
      121 aaatgggtcg acttgaacgg aaagcgctct agtgaaccgg agggggctcg ccgtcaacag
      181 gctcatagat cccatcggtt gttgcgccct gcagcgaggg ctataacccg agccttcgcg
      241 gcgggaggtg tagggcgtga ccgtgtcgag catgaccgga tttgctcgtg ctgacgggca
      301 cgaggacggc gtttcctggg tctgggaagt aaagagcgtg aacggcaagt cgctcgatct
      361 gcgcttccgt ctgcccccgg gactcgagtc gctcgaattg ccgttgcgcg ccggcatcgc
      421 caggcgcttg aagcgcggcg cgctgtccct gacgctgacg ctcacccgcg ccggcgcggc
      481 cgggggcttg cgcgtcaacc gcgaggcgct cgcgcaagtg gtggcgctgg cgcaggagct
      541 ggtggcgcag gggcgcgccg cgccgccgcg tgccgacggg cttctggcgc tgcgcggcgt
      601 cctcgaaagc ggcgacgaaa aagaagagga aggcgaccgc gagcggcgcg aggcgtcgct
      661 gctcgcgagt tccgagaccg cgctcgaccg gctcgtggcg atgcggctcg ccgagggcgc
      721 gcgcctcatt ccgctcctcg aagcgcgtct tgcggacatc gcccggctgg tcgatctggc
      781 ggagaacagc gccggcgccc ggcccgaggc attgaaggcg cggcttcagg aactggttgc
      841 cgcgctgctc gaggccagcc cgtctttgcc gccggagcgg ctcgcccagg aagcagcgct
      901 gctcgtcgcc aaagcggacg tccgcgagga gctcgatcgg ctctcggggc atatcatcgc
      961 ggctcaggag ttgctggccg agggcggtgc catcgggcgc cggctcgatt tcctttgtca
     1021 ggagttcaac cgcgaggcca atacgctctg tgccaagtcg ggcgacatgg cgctgacgcg
     1081 catcggcctc gacgtcaagg cggcgatcga tcagttgcgc gagcaggcgc aaaacgtcga
     1141 ataggccacg cgatgagcgc aagcgagatt acgaggcgcg gtattttgct ggtcctgtcc
     1201 tcgccgtcgg gtgccggcaa gaccaccatc acgcgcgagc tggtggccag ggatccgctg
     1261 ctccggatct ccgtctcggt gacgacgcgg ccgcggcggc gcgacgaggt cgatggccag
     1321 cactatcatt tcattgcgcc ggcgcaatac gatgcgatgg tcgcaaaagg cgagctcctc
     1381 gagcatgcga ccgtcttcgg ccattgctat ggcacgcccc gcgctccggt cgaggcggca
     1441 ttggccgccg gccgcgatat cgtcaccgac atcgattggc aggggacgca gcagctcaac
     1501 gggacgattc gtgccgatct ggtggcggtc ttcgtcctgc cgcccagcat ggccgcgctc
     1561 gagcagcgcc tgcggacgcg ggcccaggac agcgatgacg tcgtgcgcgg ccggatggcg
     1621 aagtcggccg acgagatgag ccattggccc gaatacgact atgtcttcgt caaccgcgac
     1681 atcgccgaga gcgtgcgcca ggtgcaatcg atcctgtcgg ccgagcgtat ccgccgcgaa
     1741 cgcctcctcg ggcttgccga cttcgtcaat cgcttgcgtg ccgggagcca agcatgaccc
     1801 ttcgcgagga ctatgagcgc tatgacgggc tcggccttgc cgcgctggtg cggcagcgcg
     1861 aggtcagtcc cggcgagttg ctcgatgccg cgatcgagcg cgtcgagcgc cgcaatcccg
     1921 caatcaatgc cgttatcgac cgcttctatg atcgggccaa ggcggccatc gcggcgggct
     1981 tgccgtcggg tccgtttacc ggcgttccct atctgctcaa ggattatggc gtctatctcg
     2041 cgggcacgcg cacgaccggc ggctcgcgct tgttccgtga cgcggtggcg gaccacgaca
     2101 gcgagatcgt cctgcgcatg aagcgcgccg gcctcgtcat cttcggcaag acgaacacgt
     2161 cggagctcgg cctctcgacc tcggtcgagc cgttgctctt cggaccgacg cgaaacccct
     2221 ggcagcgcga ccacagcgcc ggcggctcga gcggcggcgc ggccgcttcc gtcgcttcgg
     2281 gcatggtgcc gatggcccat gccacggatg gcggcggctc cattcgcatc ccggcatcct
     2341 gttgcggcct cttcggcctg aagccgacgc gcgcgcggac gccgctgggg ccggatctcg
     2401 gcgaatcctg gaacggtgcc ggcgtcgggc atgcggtcac gcgctccgta cgcgacagcg
     2461 cggcgctgct cgacgcgacg gccggtcccg atatcggcga tccctattgg gcgccgccgc
     2521 tgcgcggctc ctttctggcc gagatcggtg ccgatccggg ccggctcaag atcgccctcg
     2581 cgacgcgccc cggcaccggc gacgccgtcg atccggaatg cgtcgcggcc gccaccgctg
     2641 cggcccggct ctgcgagagc ctcgggcatg aggtggtcga ggcggcgccc gagatcgcgg
     2701 agccggagcg cagcgcggcg gcccgcgtca ttgccacggc gcacaccagc gtcactctgg
     2761 aattgcgcgc caaggcgctc ggccgcgcgc ttaggcccga tgacgtcgag ccacagacct
     2821 gggcggcagc cgagttcggc cgccaggaat cggccagcgc ttatgccaac gcgatccgcg
     2881 tgctgcaccg ggcaggccgc gtcgtcggac gcttcttcaa gcgatacgat gtactgctca
     2941 cgccgacaat gtgccgcccg ccgcacaaaa tcggcgtgat tgccgcgagc aaccgcgatc
     3001 aacgggcctt tgtcgaggcc ttgctcggca cgatcgcttt cacgtcgccc ttcaacggca
     3061 ccggcaatcc cgcgatgtcg gtgccgctcc attggtcgcc ggacggtctc cccatcggcg
     3121 tgcagttcgt ggccgggttc ggcgaagaag cgactctctt ccgtctggcg gcgcagctcg
     3181 agaaagccca gccctgggcc gaccgccgac cctcgattta gatcgcgcgc ttcttcttcc
     3241 cctctcccgc gaggagcggg agagggtggc gcgcgcgtag cgcgcgccgg gtgaggggac
     3301 ggctgcagat gaatctcgct tccgatatcc cctcaccctc ccagcgcttc gcgccgggtc
     3361 cctccctctc ccgctcatcg cgggagaggg gcaatgtgta ctcacgcggc gtcagagttg
     3421 tagcgcagct tcgcgcgccg ccacaccgcg acagggttga tcggcctcag tgtggccggt
     3481 cggcgctcgc cgtagcgctt gccgtcacgc gccagatgca gttgccgaca tcgtcggcga
     3541 cgagcagcgc gccaccgtgg tcgatggcga ctccgacggg acggccgcgc gcgttgccgt
     3601 cgccgtcgag gaacccggtg aggatgtcga tgggctcgcc cgagggacgt ccgcccgcaa
     3661 acggcacgaa gacgaccttg tagccgctgg cgggacgacg gttccaggag ccgtgctcgc
     3721 cgatgaacac gccgctcgcg aatccgatgc cgagcttggc gcggtcggcg aaggtgagcc
     3781 cgagcgccgc cacatgaggc ccgagcgcat agtccggagc gatcgcctgc gcgacgaggt
     3841 cggggcgggg cggcgtcatg cgctcatcca catgctggcc gtaatagctg tagggccagc
     3901 catagaagcc gccgtccctg accgaggtca gataatccgg caccaggtcg ctgcccagct
     3961 cgtcccgctc gttgacgacg gtccacagcg cgtcggtggc ggcctcccag gcaaggccgt
     4021 tcgggttgcg caggccggac gcgaagatcc gatggtcgcc ggtgcgcgga tcgatttccc
     4081 agatcgccgc ccggccttcc tcggcggcga tgccgtggtc gccgacattg ctgttcgagc
     4141 cgaccgtgac atagagccgc ttgccgtccg cgccggcgat gacgttcttg gtccagtgat
     4201 ggttgatcgg cccggctggc aaatccgcga ctttgacgcc cgctgcggtg atcttcgtgt
     4261 cgcccgcgtg atagggaaag cgcagcaatg cgtcggtgtc ggcgacgtag agcgcgtcgc
     4321 cgatgagcgc catgccgaag ggcgaggtca gtccttcgag gaagaccgag cttgtctctg
     4381 ccgtgccgcc gccgtcgctg cgcagcagcg tgatgcgatt ggcgctcggc acgcgcgcgc
     4441 cggcgcgctt catgatgagc tgcgcagcga agccgaaaat gccggtgtcg tcctcgggcc
     4501 ggtccggcgc attggtttcc gccaccagga cgtcgccatt gggcaggacg aagagccagc
     4561 gcggatggtc gagcttgtcg gcgaagacgg cgaccgcgag gccgggcgcc ggcgtcggcg
     4621 actcgcctgc tgcccagcct ttggccggtg cgatgttcac cgtcgggatc agcgtatggt
     4681 gcggcggcgg cagcttcgga tcgggcccga cgccggccga cagcggctgg ctcgcttcgt
     4741 cgccgcagcc ggcgaggagg agcgatgcca gcaaggccgc ggcggtcaga agaaggctgc
     4801 gcatgctctc ctcttagcac gagagcggcc ggcaatcgac ctcatcctcc gaaggcgcgg
     4861 gcgagtgcgc agaattgctc gacgccgagc tcctctgcgc gcgccgtggc agcgataccg
     4921 gtcgcggcga gcagcgcctc gacgtcgaca ccgagcgacc tgaggctctg gcgcagcatc
     4981 ttgcggcgct ggccgaaggc ggcggcgacg acgcgctcga gatccctgaa gcggcagggc
     5041 gcgaggggcg ccgcgcgcgg cagcagctgc accagcgtcg acgtcacttt gggcggcggg
     5101 acgaaggcct gcggtggaat gtcgaagagc gcgcgaacct ggcagcacca ctgcaccatg
     5161 accgagaggc ggccatagtc cctgctgcgc ggtgcggcgg cgaggcgctg cgccacctcc
     5221 ttctgaaaca tgagcgtgag gctctcatac gcgccgatcg tcgggagcca gcgcaggagc
     5281 agcgccgtgg cgatgttgta gggcagattc gcgacgatct tgcgcggctc cggcaccagg
     5341 tctctcggat cgatctcgag cgcgtcgcct tcgaccacgg cgagacgccc gggaaacgcg
     5401 gcggcgagct cttcgagagc ggcgatgcag cgccgatcgc gctcgatcgc cgtcacatgg
     5461 cgcgcgccct cgagcagcag cgcgcgggtg agcccgcccg gccccggacc gatctcgatg
     5521 atgccggcgt cggcgagcgg cgcggcttcc cgcgcgatgc ggcgggtgag gttgaggtcg
     5581 agaaggaagt tctggccgag cgccttgcgc gcaccgagct cgtagcgccg gatcacctca
     5641 cgcaggggcg gcagcgcgcg atcaggctcc gccacggaca cgatgctcct cccgcaggaa
     5701 gcgacagcaa gcgaatcggc gcgagacgac gcgcgtgaca ccattcacgc gatctcgacc
     5761 ttcgatcctg cgtctactcg cgtcggtcgg aatattcggc ttgccgctca gccttttgcc
     5821 tcttgaggaa cgccgcccgg gcgtgagcca gcctctctcg cgctgccttg gccttattct
     5881 taaaccggtg cgcgagcgct gtcacccgtt cgtagtccag atgcggctcg atcgacgatc
     5941 tggcgctccg aagaagtcga gccgccttca ggcggaaata agacgccgac atgtcaacct
     6001 cccatggcgt ctattctaga gaccgtgacg cctggcacgt acttatatgg tgacgatctt
     6061 tgaaatttac acgggtaaca aattgaaaat ccgcgcgtgc ttcggcacga atgtccacgc
     6121 gacctagcga atgatcatgc ggtagcaatc caggctgccg ctcaggaatt cataggcggc
     6181 gccccagaga taaaagcgga agcggcgata attgaactcg ccgaaacgcg cgatcactgc
     6241 ctcgcgattg tggtcgagat ttcgcgccca ttgctgaaac gtcaggaagt agctctcgcg
     6301 atcattgaag agctcgacca cccgaagcgg cgtgcgcgca agcttgtcga ggaaatcgtg
     6361 caggacgagg aacgagtggt ttccgccgta aacgtatttg accatgaagg aggatagttc
     6421 atattttctg gtcgcggcgc tgccgtcgag gaagatgggc gcgccgggct tgagcaacga
     6481 catgaatttt tcgataacac gctggtaatc cggcaggtgc tcgatcacgc ccatgatgac
     6541 gaccgcgtcg tatttgcgat cggtctcgta ttcgagca
//
LOCUS       DASYNS010000298         3419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1787159, whole genome shotgun sequence.
ACCESSION   DASYNS010000298 DASYNS010000000
VERSION     DASYNS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787159"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VGP50_09200"
     CDS             <1..241
                     /locus_tag="VGP50_09200"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015074185.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HEV8027595.1"
                     /translation="VQLEEALRLSVDAVLLDNMSLEELRRAVALVDRRAITEASGRIT
                     PETAPPIAATGVDLISVGWLTHSAAVLDIGLDYRG"
     gene            complement(434..856)
                     /locus_tag="VGP50_09205"
     CDS             complement(434..856)
                     /locus_tag="VGP50_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV8027596.1"
                     /translation="MLRMHQPGEAAFRAILPEDIDWKPFPAFPPSVRLAVLVGDPSQA
                     GPYVVRVKVPAGEKLMPHRHPEDRIYTVMCGVFYIGLGDHFDGDKVEAYPPGSVIVLP
                     GDTAHFHWAKSGGYVTQVNAIGPLGLDYLDSADDPRAT"
     gene            complement(898..1320)
                     /locus_tag="VGP50_09210"
     CDS             complement(898..1320)
                     /locus_tag="VGP50_09210"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV8027597.1"
                     /translation="MIRKAKAVWRGTGRAGSGALSTDSGVLAETPYSFRTRFENEKGT
                     NPEELIAAAHAGCFTMALAFQLQGAGYTPLELATEAAVTLAPEGQGFRISRSALTLRA
                     QVPELDESTFLRLAGEAEKNCPVSKVLNAEITLDAKLM"
     gene            complement(1575..2618)
                     /locus_tag="VGP50_09215"
     CDS             complement(1575..2618)
                     /locus_tag="VGP50_09215"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV8027598.1"
                     /translation="MGFKVAVVGATGVVGHEMLSILAERQFPVSEVYAVASERSVGAE
                     VSFGEDKVLKVQDLDRFDFKGIDIGLFSPGANVSAVHAPRAAKAGTVVIDNTSQFRMD
                     PDVPLVVTEVNPQAIAQYKKRGIIANPNCSTMQLVVALKPLHDLARIKRVVVATYQSV
                     SGAGREAMDELFNQTRSVYVNDPVKKEHFTKQIAFNVIPHIDVFMDDGSTKEEWKMAA
                     ETRKILDPDIAVTATCVRVPVFIGHAEAVNVEFERPITEGEARAALKKAPGIIVIDHR
                     ADEGYVTPQESAGEDAVYVSRIRKDPTVPHGLNLWVVADNLRKGAALNAVQIAEILAQ
                     DYLGASKKATAAQ"
     gene            complement(2709..3119)
                     /locus_tag="VGP50_09220"
     CDS             complement(2709..3119)
                     /locus_tag="VGP50_09220"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV8027599.1"
                     /translation="MKTSLKLSLALLFGIGLGIGGTQVLQAEQPAITRNVLQHADLTG
                     VPGKEVYMTVIDAAPGASFAPHIHHGDEFTYVLQGSLDLDVDGEGQRTVKAGDSIHVE
                     RDKVHGGKVAGGTPVKLLAIHIVDKGKPLADPVK"
BASE COUNT          551 a         1105 c         1146 g          617 t
ORIGIN      
        1 cgtgcagctc gaagaagcgc tgaggctcag cgtcgatgcg gtgctgctcg acaatatgag
       61 tctcgaggag ctgcgccgcg cggtggcgct ggtcgaccgc cgcgccatca ccgaggcctc
      121 gggacggatc acgccggaga cggcgccgcc gatcgccgcg acgggcgtcg atctcatctc
      181 cgtcggctgg ctcacgcaca gcgcagccgt gctcgacatc ggtctcgatt atcgcgggtg
      241 agaccctctc gccctcccgc cctctagagc agatcccgag caggtggaat tcgcactttc
      301 ccaacatcgt catccccgcg aaagcggggc ccaggggcca ccggcgcgtc gcccgccctg
      361 ggtccccgct ttcgcgggga tgacgatatg gtaggcatcg attccgtttg atcgggatgc
      421 gctctagcgc ggcctatgtt gcgcgcgggt cgtcggcgga atcgaggtag tcgaggccga
      481 gcgggccgat ggcgttgacc tgagtcacgt agccgcccga cttcgcccag tggaagtggg
      541 cggtgtcgcc ggggagaacg atgacgctgc ccggcggata cgcttccacc ttgtcgccgt
      601 cgaagtgatc gccgaggccg atatagaaga cgccgcacat gaccgtgtag atgcggtcct
      661 cgggatggcg gtgcggcatc agcttctcgc ccgccggcac tttgacccgg acgacatagg
      721 ggccggcttg cgaggggtcg ccgacaagga cggcgaggcg gacggacggc gggaacgccg
      781 gaaacggctt ccagtcgata tcttccggca ggatcgccct gaaagcggcc tcgccgggct
      841 ggtgcatccg aagcatggat gtcctccttg atcgatcgct gccggcgcgc gagcgcctta
      901 catcaacttc gcgtccaacg tgatctctgc gttgaggacc ttggagacgg gacagttctt
      961 ctcggcctcg ccggccagcc gaagaaacgt cgattcgtcg agctccggca cttgggcgcg
     1021 gagcgtcagc gccgaccggc tgatgcgaaa tccctggcct tcgggcgcga gcgtgaccgc
     1081 ggcctcggtc gcgagctcca gcggtgtata gccggcaccc tggagctgaa aggcgagcgc
     1141 catggtgaag cagccggcat gggcggcggc gatcagctct tcgggattgg tgcccttctc
     1201 gttctcgaag cgggtcctga aggaataggg cgtctcggcg agcacgcccg aatccgtgga
     1261 aagagcaccg ctgccggcgc ggcccgtgcc gcgccaaacc gccttcgcct tgcgaatcat
     1321 ccgatatctc cttgctgtcg cgtgtcgcgc cgcgtccaga ggagcggcgc atcgccatcg
     1381 tcatgaacgg agccggtgag cgccggcgat gcacgcatcg ccgcgtcgtc gggactgctt
     1441 tggaaccctt cgacgacggc ggaactttgc tgccgtcgcg atctattagc gccgacggcg
     1501 ccgtcggtcg caattgaccc agagtgtcgc cgtggttatt cgaacgtatc gccggggtgg
     1561 cgcgcggatt gccgctactg agcggccgtc gctttcttgc tcgcgccgag atagtcctgg
     1621 gcgaggatct cggcgatctg gacggcgttg agggcggcgc ctttgcgcag attgtcggcg
     1681 acgacccaca gattcaaacc gtgcggcacc gtcgggtcct tgcggatgcg gctcacatag
     1741 accgcgtcct cgccggcgct ctcctggggc gtgacatagc cttcatcggc gcggtgatcg
     1801 atgacgatga tgccgggcgc cttcttgagc gcggcgcgcg cctcgccctc ggtgatcgga
     1861 cgctcgaact cgacattgac ggcctcggca tggccgatga acacgggcac gcgcacgcag
     1921 gtagcggtaa ccgcgatgtc gggatcgagg atcttgcgtg tctcggccgc catcttccat
     1981 tcctccttgg tcgagccgtc atccatgaag acgtcgatgt gaggaatgac gttgaaggcg
     2041 atctgcttgg tgaaatgctc cttcttgacg ggatcgttca catagaccga gcgcgtctgg
     2101 ttgaagagct cgtccatggc ttcgcggccc gcgcccgaca cggattgata ggtcgcgacc
     2161 acgacccgct tgatgcgcgc cagatcgtga agcggcttca gcgccaccac cagctgcatg
     2221 gtggagcagt tgggattggc gatgatgccg cgcttcttgt attgcgcgat ggcctgcggg
     2281 ttgacctcgg tgacgacgag cggcacgtcg gggtccatgc ggaattgcga ggtgttgtcg
     2341 atcaccactg tgccggcctt ggcggcgcgc ggcgcgtgca ccgcggacac gttggcgccc
     2401 ggcgagaaaa ggccgatgtc gatgcccttg aagtcgaaac ggtcgaggtc ctgcaccttc
     2461 agcaccttgt cctcgccgaa agagacttcg gcgccgaccg accgctcgga ggcgacagca
     2521 tagacctcgg acaccgggaa ctggcgctcg gcgaggatac tcagcatctc gtggccgacg
     2581 acgcccgtgg cgccgacgac ggcaaccttg aaacccatgt catactccgg caaacgtgaa
     2641 aaacacgccc tggcgcgctt cagttacggt ctcgggcgcc ggttgacaag gggcgcccga
     2701 cgggcgggct atttcaccgg gtcggccagg ggcttgccct tgtccacgat gtggatggcg
     2761 aggagcttca ccggcgtgcc gccggccacc ttgccgccat ggaccttgtc gcgctcgaca
     2821 tggatgctgt cgccggcctt caccgtcctt tgtccctcgc cgtccacatc gaggtcgagc
     2881 gagccctgca acacataggt gaattcatcg ccatgatgaa tatgcggcgc gaagctggcg
     2941 ccgggcgccg cgtcgatgac ggtcatatac acttccttgc cgggaacgcc ggtgagatcg
     3001 gcatgctgca ggacgttgcg cgtgatcgcc ggctgctcgg cctggagaac ctgcgttcca
     3061 ccgatcccga ggccgatgcc gaacagcagg gcgagcgaca gtttcagcga cgtcttcatt
     3121 gcttcttccc cttcgtcggg tgcgtttcgg acgctagtat ggcgcccgcc cgtccgccgg
     3181 cgtagcggaa agtagaaacg cgggcggagg gatcgccgtg tatccatcgt cgtcgctcag
     3241 gaagctctga ccgtgtccgc cgacccgctc gacccgggct cgctcgcgcc gctcttccgc
     3301 ccgtcctcgg tggcggtgat cggcgcctcg accgagccca ccaagctcgg cagcgtgccc
     3361 atcgggcaca tgaaggcgag cggctttgcc ggagcgctct atcccatcaa ccccaaggc
//
LOCUS       DASYNS010000299         3615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1474307, whole genome shotgun sequence.
ACCESSION   DASYNS010000299 DASYNS010000000
VERSION     DASYNS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474307"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VGP50_09225"
     CDS             <1..483
                     /locus_tag="VGP50_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV8027600.1"
                     /translation="AFPAVGRISPDYYCVDPTIPRKRLPDVLTRMSALAAQHGLRVAN
                     VFHAGDGNLHPLILYDANKPGELAAAEACGADILRYCVEIGGVLTGEHGVGVEKRDLM
                     GTMFGAVDLEQQLRVKCAFDPEGLLNPGKQFPELHRCAELGHLHVHRGEVPFPDIERL
                     "
     gene            493..1680
                     /gene="glcE"
                     /locus_tag="VGP50_09230"
     CDS             493..1680
                     /gene="glcE"
                     /locus_tag="VGP50_09230"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEV8027601.1"
                     /translation="MTHFRPQDEQELVAIVQQALAAEEPLELIAGGSKRGIGRPLQAP
                     HVLDLGAFSGIGDYEPAELVLTAGAATPLDEIEAALDKARQMLAFEPGDWRALLGTQD
                     KAPTLGGALACNLAGPRRIRQGAARDHFLGFKAVSGRAEAFKAGGRVVKNVTGYDLCK
                     LMAGSYGTLAALTEVSVKVLPRPEARRTVALFGLDATRAVAAMTAALNSAHELTGAAH
                     LPADVARGGKAMTLARVEGPVPSVEARATALRRELAPFGDAEILGDDASLPLWRDLRD
                     VVPLAAPAEAAVWRVSIAPSAAPALIATLSRALDLGYFLDWGGGLVWVAVPQAQDGGA
                     AAIRAALGSGHATLIRAGDALRASVPVFQPQAPQLAALAARVKESFDPRRIFNRGRMV
                     AEL"
     gene            1680..3044
                     /gene="glcF"
                     /locus_tag="VGP50_09235"
     CDS             1680..3044
                     /gene="glcF"
                     /locus_tag="VGP50_09235"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEV8027602.1"
                     /translation="MQTSFSLAQLADSDTQEADKILRSCVHCGFCNATCPTYLELGDE
                     LDGPRGRIYLIKDMLEGDKPASPEVVTHIDCCLSCLSCMTTCPSGVNYMHLVDHARAY
                     IEATYRRPFGDRFIRTLLAATLPHRGRFRAAVKAARLARPLKGFLGSLPFLGARLAAM
                     LDLAPASLPAQEEITRPRVFPAAGIRKARVALLDGCVQPVLEPSINEATIRVLNRLGV
                     EVVLAKGEGCCGALVHHMGKQQAAHAYAARNIEAWHGEIEGQGLDAILITASGCGTTI
                     KDYGFMFRDDAKLAEKAAKVSALAKDICEYLTGLELPAPSRSSGLTVAYHSACSMQHG
                     QKITDEPKRLLQAAGFKVKDVAEGHICCGSAGVYNILQPEIAGKLRDRKVGHIARTKP
                     DIVATGNIGCITQIAKGFADRAKAGGPASPPIVHTIELLDWALGGPRPAGVPAAPGGT
                     GELR"
     gene            complement(3127..3531)
                     /locus_tag="VGP50_09240"
     CDS             complement(3127..3531)
                     /locus_tag="VGP50_09240"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV8027603.1"
                     /translation="MQIIDHEHETCEEWRSGVTTRMRVSSLTGTAQLCLFEQWCAPGS
                     GAPTHLHAVEEVLSVVAGRAEVWVEEERGMLGANQSLIIPAGRRHGFRNVGDTVLHVQ
                     ATLAASSFEASFDDPRELSRRWLPRSPTPELR"
BASE COUNT          560 a         1280 c         1181 g          594 t
ORIGIN      
        1 gcgttcccgg cggtcggccg catctcgccc gattactact gcgtcgaccc gaccataccg
       61 agaaagcggt tgccggatgt cctgacgcgg atgagcgcgc tcgccgcgca gcacgggctg
      121 cgcgtcgcca acgtgttcca cgccggcgac ggcaatctgc atccgctgat cctttatgac
      181 gccaacaagc cgggcgagct cgccgccgcc gaagcctgcg gcgccgacat cttgcgctat
      241 tgcgtcgaaa tcggcggcgt gctgaccggc gagcacggcg tcggcgtcga gaagcgcgac
      301 ctcatgggca cgatgttcgg cgcggtcgat ctcgagcagc agctgcgcgt caaatgcgcc
      361 ttcgatccgg aaggcttgct caatcccggc aagcaattcc cggagctgca tcgctgcgcc
      421 gagctcggcc atctccatgt ccatcgcggc gaggttccgt tcccggacat cgagcggctg
      481 taggcacccg ccgtgactca ctttcgcccg caagatgagc aagagctcgt cgccatcgtg
      541 cagcaagcgc tcgccgccga agagccgctg gaactcatcg ccggcggcag caagcgcggc
      601 atcggccgcc cgctgcaggc gccgcatgtg ctcgacctcg gcgccttttc cggcatcggc
      661 gattatgagc ccgccgagct ggtgctgacc gccggcgcgg cgacgccgct cgacgagatc
      721 gaggccgcgc tcgacaaggc gcggcagatg ctggctttcg agcccggcga ttggcgcgcg
      781 ctgctcggca cgcaggacaa agcgccgacg ctcggcggcg cgctcgcttg caacctcgcc
      841 gggccgcgcc gtatccgcca aggcgcggcg cgcgatcatt tcctcggctt caaggcggtg
      901 agcgggcgcg ccgaggcgtt caaggccggc ggccgcgtcg tcaagaacgt caccggctac
      961 gatctctgca agctgatggc cggctcctac ggcacgctcg cggcgttgac cgaggtgagc
     1021 gtcaaggtgc tgccgcggcc cgaggcgagg cgcacggtcg cgctcttcgg tctcgatgcg
     1081 acgcgcgcgg tggccgcgat gaccgcggcg ctcaactcgg cgcatgagct gaccggtgcg
     1141 gcgcatctgc cggcggatgt cgcgcgcggc ggcaaggcga tgaccctggc gcgtgtcgaa
     1201 gggccggtgc cgtcggtcga agcgcgcgcg acggcgctgc gccgcgagct cgctcccttc
     1261 ggcgatgccg aaattctcgg cgacgacgcg tcgctgccac tgtggcgcga tctccgcgat
     1321 gtcgtgccgc tcgcggcgcc cgcggaagcg gcagtgtggc gggtctcgat cgccccgagc
     1381 gccgcgccgg cgttgatcgc gacgctgagc cgcgctctcg atctcggcta tttcctcgat
     1441 tggggcggcg ggctcgtctg ggtcgccgtg ccgcaagcgc aagacggcgg cgcagccgcg
     1501 atccgcgccg cgctcggtag cggccatgcg acgctcatcc gcgccggcga tgcgttgcgc
     1561 gccagcgttc cggtgttcca gccgcaagcg ccacaactcg ccgcgctcgc ggcgcgcgtc
     1621 aaggagagct tcgatcccag gcgcatcttc aaccgcggcc gcatggtcgc ggagctctga
     1681 tgcagaccag cttttccctc gcgcagctcg cggattccga cacgcaggaa gccgacaaga
     1741 tcctgcgcag ctgcgtccat tgcggtttct gcaatgcgac ctgcccgacc tatctcgagc
     1801 tcggcgacga gctcgacggg ccgcgcggcc gcatctacct catcaaggac atgctcgaag
     1861 gcgacaagcc ggcctctccg gaggtggtca cgcatatcga ctgctgcctg tcttgccttt
     1921 cctgcatgac gacctgccct tcgggcgtca actacatgca cctcgtcgat catgcgcgcg
     1981 cctatatcga agcgacctat cggcgcccct tcggtgaccg ctttatccgc acgctgctcg
     2041 ccgcgaccct gccgcatcgc gggcgcttcc gggccgccgt caaggccgca aggctcgcaa
     2101 ggcccctcaa ggggttcttg ggctcgctcc cctttctggg tgcgcggctc gccgccatgc
     2161 tcgacctcgc tcccgcctcg ctgccggcgc aggaggagat cacgcggcca cgcgtctttc
     2221 cggccgccgg catccgcaag gcgcgtgtcg cgcttctcga cggctgcgtc cagccggtcc
     2281 tcgagccctc catcaacgag gccacgatcc gcgtcttgaa ccggctcggt gtcgaggtgg
     2341 tgctcgccaa aggcgaaggc tgctgcggcg ccctcgtgca tcacatggga aagcaacaag
     2401 ccgcccatgc ctatgcggcc cgcaacatcg aggcttggca tggggagatc gagggccaag
     2461 gcctcgatgc catcctgatc acggcgtcgg gttgcggcac cacgatcaag gattacggct
     2521 tcatgttccg cgatgacgcg aagctcgccg aaaaggccgc gaaggtctcg gcgctcgcca
     2581 aggacatttg cgagtatctc acggggctcg agctgccggc gccgagccgc tcaagcggcc
     2641 tcaccgtcgc ctatcattcg gcctgctcga tgcagcacgg ccagaagatc accgacgagc
     2701 cgaagaggct cttgcaggcg gcggggttca aggtgaagga cgtggccgag ggccatatct
     2761 gctgcggctc ggccggcgtc tacaacatcc tgcagcccga gatcgccgga aagctgcgcg
     2821 acaggaaggt cggacatatc gcgcgcacga aacccgatat cgtcgccacc ggcaacatcg
     2881 gctgcatcac ccagatcgcg aagggcttcg ccgatcgggc gaaagccggc gggccggcct
     2941 ccccgccgat cgtgcatacg atcgaactgc tcgactgggc gctgggcgga ccgcgcccgg
     3001 cgggcgtccc cgccgctccc ggaggcaccg gtgaattacg gtgacagtgc acttaattcc
     3061 aggaattccc ctttacctcc gggtgttgcc agaattaagt gcactgtcac cgtaattcac
     3121 accccatcac cttaattccg gcgtcgggct tcgcggcagc cagcggcgcg acagctcgcg
     3181 cggatcgtcg aaggaggcct cgaagctcga ggcggcgagc gtcgcttgca cgtgcagaac
     3241 ggtgtccccg acattccgga agccgtgccg gcgtcccgcc gggatgatca gcgactggtt
     3301 ggcgccgagc attccccttt cctcctccac ccagacctcg gctcgcccgg cgacgacgct
     3361 cagcacctcc tcgaccgcat gcagatgggt cggggctccg cttcccggcg cgcaccattg
     3421 ctcgaagagg caaagctgcg cggtgcctgt gagcgaggag acgcgcatgc gcgtggtgac
     3481 gcccgaacgc cattcttcac aggtctcatg ctcgtgatcg atgatttgca tgcgtcatcc
     3541 ccttcctcgt cgcgggccga agccttgcct gtaaagcctt gcctgtttcg tcaccttcgc
     3601 ctgcgcccaa aggag
//
LOCUS       DASYNS010000300         2563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1318059, whole genome shotgun sequence.
ACCESSION   DASYNS010000300 DASYNS010000000
VERSION     DASYNS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318059"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2221
                     /locus_tag="VGP50_09245"
     CDS             <1..2221
                     /locus_tag="VGP50_09245"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEV8027604.1"
                     /translation="RRVATALAAVEREPQPWAERFYEALEGFKFLPAGRLVAGAAAGR
                     NVTLFNCFVMGEIPDDMSGIFRHLREAALTMQQGGGIGYDFSTLRPKGAPVKGVGADA
                     SGPLSFMDVWDAMCRTIMSAGHRRGAMMATLRCDHPDIEAFIEAKREAGRLRMFNLSV
                     LATDAFMTAVKEDAPWELAFAGTTYKVVPARELWDKIMRSTYAYAEPGVIFIDRINRR
                     NNLWYCETISATNPCGEQPLPPYGACLLGSINLAALVRDPFAPQARLDMDELERLVPL
                     AVRMLDNAIDVSRFPLEEQAKEAKAKRRIGLGITGLADALILCGARYGSSDAVALTEQ
                     WMKAIQREAYLASAALAAEKGAFSLFDKEQYLAGETVAALDVDLRSAIAAHGIRNALL
                     TSVAPTGTISLFADNVSSGLEPVFSFTYTRRVLMPDGSRKEEEVSDYAYRLFRRSKGT
                     GAPLPAAFVDAQQLSPADHLVMQATVQKYVDSSISKTINIPADFPFERFKDVYLQAYE
                     LGCKGCTTYRPNEVTGAVLETRKETRGERQAQLPLPAPAAAKAARPADVYDGGVVYMT
                     QPLDRPEELPGKTYKIRWPESEHALYITLNDIIQDGRRRPFEIFINSKNMEHYAWTVA
                     LTRMVSAVFRRGGDVSFVVEELKAVFDPRGGAWMEGRYVPSLLAAIGDVIERHLIDIG
                     FLPAKRSATPELIERKVVNLPEHEGRRLAQCPKCGEAALIRVEGCDQCTSCDYSKCG"
     gene            complement(2364..>2563)
                     /locus_tag="VGP50_09250"
     CDS             complement(2364..>2563)
                     /locus_tag="VGP50_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027605.1"
                     /translation="RRVEAVGLDKAQRVVILGEAQLLIVQELAQGLADQGMIIGNQDF
                     HRDHNLYVFLALLTLYHGISR"
BASE COUNT          455 a          861 c          818 g          429 t
ORIGIN      
        1 gcgtcgcgtc gccaccgcct tggccgcggt cgagcgcgag ccccagccct gggccgagcg
       61 cttctacgag gcgctcgagg ggttcaagtt cctcccggcc ggccgcctcg tggccggagc
      121 cgctgccggg cgcaacgtca cactcttcaa ctgcttcgtc atgggcgaga tcccggacga
      181 catgtcgggc atcttccgcc atttgcgcga ggccgcgctc accatgcagc aggggggcgg
      241 catcggctac gacttctcca cgctgcgtcc caagggtgcg ccggtgaagg gcgtcggtgc
      301 cgacgcctcg ggcccgctct cgttcatgga tgtgtgggac gccatgtgtc ggaccatcat
      361 gagcgccggc catcgtcgcg gcgcgatgat ggcgacgtta cgttgcgacc atccggacat
      421 cgaggccttc atcgaggcga agcgcgaggc cgggcggctc cgcatgttca atctgtcggt
      481 gctggcgacc gacgctttca tgacggccgt caaagaagac gcgccctggg agctggcctt
      541 cgccggcacg acctacaagg tggttccggc gcgcgagctc tgggacaaga tcatgcgctc
      601 gacctatgcc tatgccgagc cgggcgtgat cttcatcgac cgcatcaacc gccgcaataa
      661 tctctggtac tgcgagacta tcagcgcgac caatccctgc ggcgagcaac cgctgccgcc
      721 ctatggcgcc tgcctcttgg gctcgatcaa tctggcggcg ctggtacgcg acccctttgc
      781 cccgcaggca cgcctcgaca tggacgagct cgagcgtctg gtgccgctcg ccgtgcgcat
      841 gctcgacaac gccatcgacg tctcgcgctt tccactcgag gagcaggcga aggaggcgaa
      901 ggcgaagcgg cgtatcggcc tcggcatcac cggcctcgcc gatgcgctca tcctgtgcgg
      961 cgcgcgctac ggctcgagcg acgccgtggc gctcaccgag caatggatga aagcgatcca
     1021 gcgcgaggcc tatctcgcct cggccgcgct cgccgccgag aaaggcgcct tctcgctctt
     1081 cgacaaggag caatacctcg ccggcgaaac ggtggcggcg ctcgatgtgg atctgcgcag
     1141 cgcgatcgcc gcgcatggca tccgcaacgc gctcctcacc tcggtggcgc ccaccggcac
     1201 catctcgctc ttcgccgaca atgtctcctc cgggctcgag ccggtgttca gcttcaccta
     1261 tacgcggcgc gtgctcatgc ccgacggcag ccgcaaggag gaggaggtga gcgactacgc
     1321 ctatcgtctc ttccgccgct cgaagggcac gggcgcgccg ctgccggccg ccttcgtcga
     1381 tgcgcagcag ctttcgcccg ccgatcacct ggtgatgcag gcgacggtgc agaaatacgt
     1441 cgacagctcg atctcgaaga ccatcaacat tcccgccgac ttccccttcg agcgcttcaa
     1501 ggacgtctat ctccaggcct atgagctcgg ctgcaaaggc tgcaccacct accgccccaa
     1561 cgaagtgacc ggcgccgtgc tcgagacgcg gaaagagacg agaggcgagc gccaggcgca
     1621 gctgccgctg ccggcgcccg ccgcagcgaa ggcggcgcgg ccggccgacg tctatgatgg
     1681 cggcgtcgtc tacatgacac agccgctcga tcggcccgag gagctgccgg gcaagaccta
     1741 caagatccgc tggcccgaga gcgagcatgc gctctacatc accttgaacg acatcatcca
     1801 ggacggccgc cgccgcccct tcgagatctt catcaactcg aagaacatgg agcattacgc
     1861 gtggaccgtc gccttgacgc gcatggtgag cgccgtgttc cggcgcggcg gcgatgtgag
     1921 cttcgtcgtc gaggaattga aggcggtgtt cgacccgcgc ggcggcgcct ggatggaggg
     1981 gcgctacgtg ccctcgctgc tcgccgcgat cggcgacgtc atcgagcgac atcttatcga
     2041 catcggcttc ctgccggcca agcgcagcgc cacgcccgag ctgatcgagc gtaaagtggt
     2101 gaacctgccg gagcacgagg gccgtcgcct cgcgcaatgc cccaaatgcg gcgaggccgc
     2161 cctcatccgc gtcgagggct gcgaccagtg cacgagctgc gactattcga aatgcgggtg
     2221 aggcgagcgg gtaagcctgc accgaatcga tgcacgcgaa tgcatgcatt cccccagtaa
     2281 agaatgctgc gttgtaagcg aagcgttaaa tcacaccgaa tagtgtaagc cgatcgagcg
     2341 ggctgtccta agccgatagg atgtcagcga gatatgccat gataaagtgt caatagcgct
     2401 aaaaacacgt ataggttgtg gtcgcgatga aagtcttgat tgccgatgat catgccttgg
     2461 tccgccaggc cctgcgccag ctcctggacg atgaggagct gggcctcgcc aagaatgacc
     2521 acgcgctgag ctttatcgag gccgacggct tcgacgcggc gct
//
LOCUS       DASYNS010000301         4656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4750496, whole genome shotgun sequence.
ACCESSION   DASYNS010000301 DASYNS010000000
VERSION     DASYNS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4750496"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3294
                     /gene="tssM"
                     /locus_tag="VGP50_09255"
     CDS             <1..3294
                     /gene="tssM"
                     /locus_tag="VGP50_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEV8027606.1"
                     /translation="EADEEITHLRKRLEESLGLLKKGAGNKGRGRYLYQLPWYMMIGP
                     PGSGKTTALQNSGLNFPLAAKYGKDPVKGVGGTRNCDWWLTDEAVLLDTAGRYTTQDS
                     HASTDQAGWKGFLGLLKKFRPRQPINGALVAISVSDVVALSSGERMAHARAIRQRLRE
                     LSDEFGIRFPVYVFLTKADLIAGFVEFFDDLGKEEREQVWGTTLVMDKGGEDEPPAVA
                     GFAAGFDALVQRLNERQLARIQQETDLNKRAMIYGFPAQVASLKDLLKEFLDEIFMPN
                     RYEGRPLLRGVYFTSGTQEGTPIDRIMAGVAANFGLDRQRLSAFSGAGRGYFLSTLLR
                     NVIFSEASVASADSKHERRQALLRMGAYGAAALVFVAALVAWTVSYFGNRSLVADIDN
                     QVAQYAQAVQPLAQTRVVDDDVARLVPTLDLLRNIPAGYGARNDSVPWSVSFGLYQGD
                     KLGAQEILAYHRALNAVFLPRLLVRLGAQIGTNLDKPDYIHEALKTYLMLGSAGPLDK
                     DLVHAWMDLDWQARYPGPANEPLRNDLMSHLNALLDAPLQQIALDGNVIDAARRALLQ
                     APLASRAYNSMKASAAAHKIQDWRISANAGPSADRVFVRRSGKLLSDGIPGFYTYEGY
                     YKVFLPGLADVALTMARERWVLGSAAPQGSEASLSSSLQQDIAKLYTADYIRQWDGLL
                     ADLTIVPFRGLQSAADATNVLSSPTLSPLKLLWTAIAKETQLSRPPDLGAAGQLAAAA
                     QQASAAATAAANKLSAVVGTPAVAALTPSYGQAVDDHFRRLQEFVGLGAPPGQPAPID
                     ALVARLNEVYVQLNTVAQNPSAPPPAGGAVANLATDATRLPPPLDALMAGVAGGALGQ
                     SSSRTHAQLNEQWQSQVVPLCQQALENRYPMVRGSQVDVTLDDFAKLFAPNGLIDGFF
                     KTNLKPFVDTSRTPWQWQRVDNLDLGISPDVLVQFQRAAAIRDGFFAAGAAPGVKFEM
                     VPVELDANATQVLIDINGQELSYAHGPTRPTLVQWPGAPGSEGGRVAFSPAAADKPAT
                     ITKQGPWSLFRLFDEARIQRSGSGLSDKLEVTFSIGGRTAVYEIQANSVKNPFLMPEL
                     HEFRCPARL"
     gene            3291..4001
                     /gene="tagF"
                     /locus_tag="VGP50_09260"
     CDS             3291..4001
                     /gene="tagF"
                     /locus_tag="VGP50_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR03373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HEV8027607.1"
                     /translation="MNEVTVAPRLGFFGKLPARGDFVCRRLPRDFTDPWDGWLQDAVA
                     MSSQQLGQAWLDTYLTAPIWRFLLARGVCGERPMLGVMMPSVDRVGRYFPLTMAVAIG
                     ESAAPARTMLTGGAWFEEVEKLALSALEDDADLDRLDQEAESIAPPAGDESSGAAYHA
                     ERAVSVVLSSDRGVAASAADVMDGLLALIAGRYTLWWTSGSEQFAPSLVVAEGLPAVE
                     RFAALLDGRWEQWGWKTR"
     gene            3986..>4656
                     /locus_tag="VGP50_09265"
     CDS             3986..>4656
                     /locus_tag="VGP50_09265"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEV8027608.1"
                     /translation="MEDTVRHSIAPHPVAAPGFRFASAAVTDVGKVRKVNEDAFLERP
                     DLGLWAVADGVGGASQGDRASRLVVEALGRVHKPAAAASFLTEVCDQLKTVNNQLQQE
                     AEQQGGHRVSASTVVTLLVFGGHFACAWAGDSRLYQMRDYRLRQISRDHSEVQELVDQ
                     GLLTAEQARTHPRANVVTRAVGAHEKLAIDMVQDRVRQDDIFLLCSDGLTKMLEDREI
                     AALLGA"
BASE COUNT          753 a         1588 c         1544 g          771 t
ORIGIN      
        1 gaggcggatg aggagatcac gcatctgcgc aagcggctcg aggaatcgct ggggctgctc
       61 aaaaagggcg cgggcaacaa ggggcgcggt cgttacctct atcagcttcc ctggtacatg
      121 atgatcggac cgcctggctc gggcaaaacg acggcgttgc agaattcggg gctgaacttt
      181 ccgctcgcgg cgaaatacgg caaggatccg gtcaaaggtg tcggcggcac gcgcaattgc
      241 gactggtggc tgactgatga ggccgtgctg ctcgacacgg ccggccgcta caccacgcag
      301 gacagccacg ccagcaccga ccaggcggga tggaagggct ttctcggcct gctcaagaag
      361 ttccggccgc gccagccgat caacggcgcg ctcgtcgcga tcagcgtctc cgacgttgtc
      421 gcgctgtcga gcggcgagcg gatggcgcat gcccgcgcca ttcgccagcg cctgcgcgag
      481 ctcagcgacg agttcggcat ccgtttcccg gtctatgtct tcctcaccaa ggccgacctg
      541 atcgcgggct tcgtcgaatt cttcgacgac ctcggcaagg aggagcgcga gcaggtctgg
      601 ggcacgacgc tggtgatgga caaaggcggc gaggacgagc cgccggccgt cgccggcttc
      661 gccgcgggct tcgacgcgct ggtgcagcgg ctcaacgagc gccagctcgc gcgcatccag
      721 caggagaccg atctcaacaa gcgggcgatg atctatggct tcccggcgca agtagcgtcg
      781 ctcaaggacc tgctgaagga gttcctcgac gagatcttca tgcccaaccg ctatgagggg
      841 cggccgttgc tgcgcggcgt ctacttcacc agcggcactc aggaaggcac gccgatcgac
      901 cgcatcatgg ccggcgtggc cgcgaatttc ggtcttgacc ggcagcgtct ctcggctttc
      961 agcggcgccg gacgcggtta ttttttgtcg acgctgctgc ggaacgtgat tttctcggaa
     1021 gcgagcgtcg ccagcgccga ttcgaagcat gaacggcggc aggccttgct gcgcatgggc
     1081 gcctatggcg cggccgccct cgtcttcgtc gctgcgttgg tcgcctggac ggtgagctat
     1141 ttcggcaacc gcagtctcgt cgccgatatc gacaatcagg tcgcgcaata tgcccaggcg
     1201 gtgcagcccc tcgcccagac gcgcgtggtc gacgacgatg tcgcgcgcct ggtgccgacg
     1261 ctcgacctgc tgcgcaacat cccggcgggc tacggtgcgc gcaacgacag cgtgccttgg
     1321 tcggtgagct tcggcctcta tcaaggcgac aagctcggcg cgcaggagat cctcgcttat
     1381 caccgcgctc tcaacgccgt cttcctgccg cgccttctcg tgcggctcgg cgcgcagatc
     1441 ggcaccaatc tcgacaagcc cgattacatc catgaggcac tcaagaccta tttgatgctc
     1501 ggcagcgccg gcccgctcga caaggacttg gtgcatgcct ggatggatct cgattggcag
     1561 gcgcggtacc cgggtcccgc caacgagccg ctgcgcaacg acctcatgag tcatctgaac
     1621 gcgctgctcg atgcaccgct ccagcagatc gcgctcgacg gcaatgtcat cgacgccgcg
     1681 cggcgcgcct tgctgcaggc gccgctggcg agccgcgcct acaattcgat gaaggcaagc
     1741 gccgcggcgc acaagattca ggactggcgg atctccgcca atgccggtcc ctcggccgat
     1801 cgcgttttcg tgcgccgctc cggcaagctg ctgtcggacg gaattcccgg cttctatacc
     1861 tatgagggct attacaaggt cttcctgccc ggcctcgccg acgtcgccct gactatggcg
     1921 cgggagcgct gggtgctcgg cagcgcggca cctcaaggca gcgaggccag cctctcgagc
     1981 agcctccagc aggacatcgc caagctctac accgctgact acatccgaca gtgggacggg
     2041 ctccttgccg acctcaccat cgtgcctttc cgcgggctcc agagcgcggc ggacgcgacc
     2101 aacgtgctct cgagcccgac gctgtcgccc ttgaagctgc tctggacggc catcgccaag
     2161 gagacgcagc tctcgcggcc gcccgatctc ggtgccgcgg gccagctcgc cgcagcggcg
     2221 cagcaagcga gcgccgccgc cacggcggcg gccaacaagc tctcggccgt cgtcggcacg
     2281 cccgcggtcg cggcgttgac gccgagctac ggccaggcgg tggacgatca cttccgtcgc
     2341 ctccaggaat tcgtcgggct gggcgccccg ccgggtcagc ccgcgcccat cgatgcgttg
     2401 gtcgcccggc tcaacgaagt ttatgtgcaa ctcaataccg tcgctcagaa tccctcggca
     2461 cctcccccgg ccggtggcgc ggtggcgaat ttggcgaccg acgcgacgcg tctcccgccg
     2521 ccgctcgacg ccctcatggc cggcgtggcg ggcggtgcgc tcgggcagag cagcagccgc
     2581 acccatgcgc agctcaacga gcaatggcaa tcgcaggtgg tgcccctctg ccagcaggcg
     2641 ctcgagaatc gctatccgat ggtccgcggc agccaagtcg atgtgacgct cgacgatttc
     2701 gccaagctct ttgcgcccaa cggcctcatc gacggcttct tcaagaccaa tctcaagccc
     2761 ttcgtcgaca cctcgcgcac accctggcag tggcagaggg tcgacaacct cgatctcggc
     2821 atctcgccgg acgtgctcgt ccagttccag cgcgccgcgg cgatccgcga cggcttcttc
     2881 gccgccggcg ccgcgcccgg cgtgaagttc gagatggtgc cggtcgagct cgacgccaac
     2941 gccacgcagg tgctgatcga catcaacggg caagagctct cctatgcgca cgggccgacg
     3001 cgtccgacgc tcgtgcaatg gccgggggcg ccggggagcg agggcggccg cgtcgcgttc
     3061 tcgccggcag cggcggacaa gcccgcgacg atcaccaagc aagggccgtg gtcgctgttc
     3121 cgcctcttcg acgaagccag gattcagcgc agcggcagcg gcctttccga taagctcgag
     3181 gtgacattct cgatcggcgg ccgcaccgcg gtctatgaga tccaggcgaa cagcgtcaag
     3241 aatccgttcc tcatgcccga acttcacgaa tttcggtgtc ccgcccgcct atgaatgaag
     3301 tcacggtcgc tccgcggctc ggcttcttcg gcaaattgcc cgcgcgcggc gatttcgtct
     3361 gccgccgact gccgcgcgat ttcaccgatc cctgggacgg ctggctgcag gacgccgtcg
     3421 ccatgagttc gcagcagctc gggcaggcct ggctcgacac atatctgacg gcgccgatct
     3481 ggcggttcct gctcgcgcgc ggtgtctgcg gcgagcggcc gatgctgggc gtgatgatgc
     3541 ccagcgtcga tcgagtcggc cgctacttcc cgctgaccat ggccgtcgcc atcggcgaga
     3601 gcgcggcgcc ggccagaacc atgttgacgg gcggcgcctg gttcgaggag gtggagaagc
     3661 tcgcgctctc ggcgctcgag gacgatgccg acctcgatcg gctcgatcag gaagcggaga
     3721 gcatcgcgcc gcccgcgggc gatgaatcga gcggcgccgc ctaccatgcg gagcgcgccg
     3781 tgtcggtcgt cctgagcagc gaccgtggcg tcgccgcgtc ggccgcggat gtcatggatg
     3841 gtttgcttgc gctgatcgcc ggccgctata cgttgtggtg gaccagcggg tccgagcagt
     3901 tcgcaccgtc gctggtcgtc gccgaagggc tgccggctgt cgaacgcttc gccgctctcc
     3961 tggatggacg atgggagcag tggggatgga agacacggta aggcactcga tcgctccgca
     4021 ccccgtggcg gcccccggtt tccgcttcgc ctcggctgcc gtcaccgatg tcggcaaggt
     4081 ccggaaggtc aacgaggacg ccttcctcga gcgcccggat ctcggcttgt gggcggtcgc
     4141 cgacggtgtc ggtggcgcga gccagggcga ccgcgcgagc cggctcgtcg tcgaggcgct
     4201 cggccgcgtc cacaagccgg cggccgccgc atccttcctc accgaggttt gcgaccagct
     4261 gaaaaccgtg aacaaccagc tccaacaaga agcggagcag cagggcggcc accgggtgag
     4321 cgcgagcacg gtcgtgacgc tgctcgtctt cggcgggcat ttcgcctgcg cctgggcagg
     4381 cgacagccgg ctctaccaga tgcgcgacta ccgccttcgt caaatcagcc gcgatcacag
     4441 cgaggtccag gagctggtcg atcaagggtt gctgacggcc gagcaggcac ggactcatcc
     4501 gcgggcgaat gtggtaactc gggcggtcgg cgctcacgag aagctcgcca tcgacatggt
     4561 tcaagaccgc gtgcgccagg atgacatctt cctgctgtgc agcgacggcc tcaccaagat
     4621 gctggaggac cgcgagatcg ccgcgctgct cggcgc
//
LOCUS       DASYNS010000302         6948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1789070, whole genome shotgun sequence.
ACCESSION   DASYNS010000302 DASYNS010000000
VERSION     DASYNS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1789070"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1610)
                     /locus_tag="VGP50_09270"
     CDS             complement(216..1610)
                     /locus_tag="VGP50_09270"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEV8027609.1"
                     /translation="MRLRAVLMTAAAMLALAIGAVRADPRGEGGVLPAGSDIDNDSFY
                     QPREVFRSEAIHGRKPYLVVLGDIAFGAPSLLGGAARQAGMSCGTCHIAGTNNPALYI
                     PGLSTRGGNFDTTNALFNAKADNGLLDPVAIPSLRGARQFGPYGHDGRMASLRDFVRQ
                     VIVVEFAGPEPAPATLDALVAYIQDIDILANRRLGEDGKLLAPVSEAEKRGEALFSKP
                     FPHDPSLSCAACHRPSAGFVDHVQHDVGSSGLFKTPTLLDANFNAPYFHDGRYRSYGE
                     VVAHFDRLFYLGLTAGDRADLVAYLQAIGGADEPYVADSIELRLAEIDDFTGALDTAM
                     PEHDTTAVALTVDAVGADLRELGENFPERKDTTVSGGVAERRAARGAVRELVLSLDRL
                     GALAGDGQFAEAAAALADYRAKRAQALPLLKAAESWSLFNPTIHEQRRAALRQVTKAG
                     VDPRLAARPRLDND"
     gene            complement(1622..2410)
                     /locus_tag="VGP50_09275"
     CDS             complement(1622..2410)
                     /locus_tag="VGP50_09275"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HEV8027610.1"
                     /translation="MRSPFRAALLAVGLAGTGLSSGSAAAAVPALDQAAQSFKPYVAK
                     RVEECLAAAQELRERMAAADLAGAQHAWIAARAGWESVETVSDEFFPELDAAIDAWPD
                     ATSGFHAIEARLFGAHRTDALPQAETLIANLKAFEEKLRGTALSAQGVLNGTARLAYE
                     MGESKADGGESPFSGNSYAEIGDNIAGIEAAYSTVFAAALKDADAASDKAVEGAVGAL
                     AALVKASDSRSIDRTRLRRLGEDLVLALTAAAPALGLEKPILGD"
     gene            complement(2579..2797)
                     /locus_tag="VGP50_09280"
     CDS             complement(2579..2797)
                     /locus_tag="VGP50_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027611.1"
                     /translation="MLSRPEGSLVSSERDVIFEFVQVGGVVKVTALDTASGIEASIVG
                     DPSAGDAALKRLARKKLDYVLAKRGAQI"
     gene            2883..4091
                     /locus_tag="VGP50_09285"
     CDS             2883..4091
                     /locus_tag="VGP50_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV8027612.1"
                     /translation="MSPPEARHTDQPRAKAVFDWQDPLLLEEELSEDERMVRDAARAY
                     AQGRLMPRVKSAFREERFDREIMSEMGELGLLGPTIDGYGCTNVGYVCYGLIAREVER
                     VDSGYRSAMSVQSSLVMYPIYDFGTEAQRQKYLPKLARGEWIGCFGLTEPDHGSDAGG
                     MKSRARKVDGGYLLKGAKMWISNSPVADVMVVWAKTEEEGIRGFILERGMKGLTTPKI
                     EGKFSLRASITGEIVMEDVFVPEENLLPGVSGLKGPFSCLNNARYGIAWGALGAAEFC
                     WHAARDYTLSRTAFGKPLAANQLIQKKLADMQTEITIGLHACLRLGRLMDEHRATPEM
                     VSMLKRNSCGKALDIARMARDMHGGNGIVDEYHVIRHVLNLETVNTYEGTHDIHALIL
                     GRAQTGLAAF"
     gene            4174..4968
                     /locus_tag="VGP50_09290"
     CDS             4174..4968
                     /locus_tag="VGP50_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027613.1"
                     /translation="MVDRTACVAFLLLALALSAMPERATAGPPYLTDDPDPVDYGHWE
                     AYLFSTGTAVHGDAAGVLPAVEVNYGALPNLHLHIQIPVAYNSQSLTGTQFGMGDASF
                     GAKYRFITADKQDWWPEVATFPMIAVPTGDEARSLGTGRTHVFLPLWALKSFGLWTTY
                     GGGGYWINPGPGNRDFWFAGWLLQRQVTDDFALGVELFHQSASATGGPGSVGFPLGSK
                     DTTGFNIGGIYDLSEHYHLLMSAGRGFQNASDSNELSYYVALQWTF"
     gene            complement(5108..5587)
                     /locus_tag="VGP50_09295"
     CDS             complement(5108..5587)
                     /locus_tag="VGP50_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEV8027614.1"
                     /translation="MRTAYLDIIALVERSHRQYLEVVKLELDAQGVHDINNVQAMILF
                     NIGDLEMTVGELTLRGCYLGSNVSYNVKKMVEADYLVQERSAHDRRSVRVRLSEKGQA
                     LRQKIVKMHERQIDALSKGPLTEQDLTAAFAALRRLDRFWTTTVELGARAVQFPPAA"
     gene            complement(5678..6580)
                     /gene="mmsB"
                     /locus_tag="VGP50_09300"
     CDS             complement(5678..6580)
                     /gene="mmsB"
                     /locus_tag="VGP50_09300"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEV8027615.1"
                     /translation="MKIGFIGLGNMGGPMAANLVKAGYDVTGFDLAEPAVAALAKAGG
                     KPAANIAATVAAADMVLTMLPAGPQVRAAHGGAAGVLAAAKPGALLVDSSTIDVESAR
                     ALIKAAAARGFDMLDAPVSGGVAGAAAGTLTFMVGGSASGFERALPVLQAMGKTIVHA
                     GPAGNGQAAKICNNMMLAVSMIGVCEGFALADRLGLDRQKLFDISSKSSGQCWAMTSY
                     CPVPGPVPASPANRDYEAGFTADMMLKDLKLAQQAAQAAGAATPLGAEAGALYQMLCN
                     AGLGPKDFSAIFGLVAGTPSTIGG"
     regulatory      6737..6798
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
BASE COUNT         1187 a         2289 c         2299 g         1173 t
ORIGIN      
        1 agcgccgccg ccatccacgg cttgctcctc cgcctcacgg atcgaaatct catggtgctc
       61 tctccctcct gtgctgctgg tgctcggaca cccgggacgg aactccgccc gagattccga
      121 gcgttgatac cctcgggtct aaagaaaaat tccgtaacga atatttgagt tcattgtcac
      181 aatacagtaa ttctccaata aatagtccgt ctccctcagt cattgtccag acgcggccgc
      241 gccgcaaggc gcgggtctac gcctgccttc gtcacctgcc gcagcgccgc gcgccgctgc
      301 tcatggatcg tcgggttgaa caaggaccag gactcggccg ccttgagcag cggcaaggct
      361 tgtgcacgct tcgcgcgata atcggcgagc gccgcggcgg cctcggcgaa ttgcccgtcg
      421 ccggcgagag cgccgaggcg gtcgaggctc agcaccagct cgcggacggc gccccgcgcc
      481 gcgcgccgtt ccgcgacgcc gcccgagacg gtggtgtcct tgcgctcggg gaaattctcg
      541 ccgagctcgc gcaaatcggc gcccaccgcg tcgacggtca gcgccacggc ggtcgtgtca
      601 tgctccggca tcgcggtgtc gagcgcgccg gtgaaatcgt cgatctcggc caggcgcagc
      661 tcgatgctgt cggcgacgta gggctcgtcg gcgccgccga tcgcttgcag ataggcgacg
      721 agatccgcgc ggtcgcccgc cgtcagcccg agatagaaca ggcgatcgaa atgcgcgacg
      781 acctcgccat agctgcgata gcgcccgtca tggaaatagg gcgcgttgaa attcgcatcg
      841 agcagcgtcg gcgtcttgaa caggcccgag ctcccgacat cgtgctggac atgatcgacg
      901 aagccggcgg aggggcgatg gcaggcggcg cagctcaggc tcggatcgtg cgggaagggc
      961 ttcgagaaca gcgcctcgcc gcgcttttcc gcctcgctta cgggcgcgag cagcttgccg
     1021 tcctcgccga gccggcgatt ggccaggatg tcgatgtcct ggatataggc gacgagcgcg
     1081 tcgagggtcg ccggcgcggg ctcggggccg gcgaattcga cgacgatgac ctggcgcacg
     1141 aaatcgcgca gcgacgccat gcgcccgtca tggccgtaag gcccgaattg ccgcgcgccg
     1201 cgcaggctcg ggatggcgac gggatcgagc aagccattgt cggccttggc gttgaagagc
     1261 gcgttggtcg tgtcgaaatt gccgccgcgc gtcgacaacc ccgggatata gagcgcggga
     1321 ttgttggtgc cggcgatatg gcacgtgccg cagctcatgc cggcctggcg cgcggcgccg
     1381 cccaggagcg acggcgcgcc gaaggcgatg tcgccgagga cgaccagata gggcttccgg
     1441 ccatggatcg cctcggagcg gaagacctcg cgcggctgat agaacgaatc gttgtcgatg
     1501 tcgctgccgg ccggaagcac gcccccttcc ccgcggggat cggcgcgcac ggcgccgatc
     1561 gcgagcgcga gcatggcggc ggcggtcatc aggacggcgc gaaggcgcat ggctcggccc
     1621 atcaatcgcc gaggatgggc ttttcgagcc cgagagcggg cgcggcggcg gtcaaggcca
     1681 ggacgaggtc ctcgcccagc cggcgcagcc gcgtgcggtc gatgctccgg gagtcgcttg
     1741 ccttgacgag cgcggcgagc gcgccgacgg caccctcgac cgccttgtcg gaggctgcat
     1801 cggcgtcctt gagcgccgcg gcgaagacgg tgctgtaggc cgcctcgatg ccggcgatgt
     1861 tgtcgccgat ctcggcatag gagttgccgc tgaacggcga ctcgccgcca tcggccttgc
     1921 tctcgcccat ctcgtaggcg agtcgcgccg tgccgttgag gacgccttgc gcggagagcg
     1981 cggtgccgcg cagcttctcc tcgaaggctt tcagattggc gatcaacgtc tcggcctgcg
     2041 gcagcgcatc ggtgcgatgg gcgccgaaaa gccgcgcctc gatggcgtga aagccgctcg
     2101 tcgcatcggg ccaggcgtcg atcgcggcat cgagctcggg aaagaattcg tcggacacgg
     2161 tctcgacgct ttcccagccg gcgcgcgcgg cgatccaggc atgctgcgct cccgcgaggt
     2221 cggcggcggc catccgctcg cgcagctcct gcgccgcggc gaggcactcc tcgacgcgct
     2281 tggcgacata gggcttgaag gactgggcag cctgatcgag cgcgggaaca gcggcggccg
     2341 cgctcccgct cgaaagcccg gtacccgcga gcccgaccgc tagaagggcc gcgcggaaag
     2401 gtgaacgcaa ccgtaagctc ctgtcatgca ccagtgattg cctcgagcgc cgagtttttt
     2461 tctgggcgag cgatggcggg agttagacag gttatctttg caggtttatg acagaccgat
     2521 gacgaattgg tcctcgtccg ggctccggtt ttcacagccg cgcgagatgc gatcttcgct
     2581 agatttgggc gccccgtttc gccagcacat agtcgagctt tttgcgcgcg aggcgcttca
     2641 gcgcggcgtc gccggcggag gggtcgccga cgatcgacgc ctcgatgccg ctcgccgtgt
     2701 cgagcgccgt caccttgacg acgccgccga cctggacgaa ttcgaagatg acgtcgcgct
     2761 cgctgctcac aagggacccc tcggggcggc tcagcatagc cgcctcgccc gcctcgcggg
     2821 agggttgccc gatgcggcgt ttgcttctat gaccgcgtcg ccgagcctca aggagccgac
     2881 agatgagccc gccggaagcc cgccacacgg accagccccg cgccaaagcg gtcttcgact
     2941 ggcaggatcc gctgctcctc gaggaggagc tcagcgaaga cgagcgcatg gtgcgcgatg
     3001 ccgcccgcgc ctacgcgcag ggcaggctca tgccgcgggt caagagcgcc ttccgcgagg
     3061 agcggttcga ccgcgagatc atgagcgaga tgggcgaact cggtctcctt ggaccgacca
     3121 tcgatggcta tggctgcacc aatgtcggct atgtctgcta cggcctcatc gcgcgcgagg
     3181 tcgagcgcgt cgacagcggc taccgctcgg ccatgagcgt gcaatcctcg ctggtgatgt
     3241 acccgatcta cgacttcggc acggaggcgc agcgccagaa atatctgccg aagctcgctc
     3301 gcggcgaatg gatcggctgc ttcggcctga ccgagcccga tcacggctcc gatgccggcg
     3361 gcatgaagag ccgggcgcgg aaggtcgatg gcggctatct cctcaagggc gccaaaatgt
     3421 ggatcagcaa ttcgcccgtt gccgacgtca tggtggtgtg ggcgaagacc gaggaggaag
     3481 gcatccgcgg cttcatcctc gagcgcggca tgaagggact gacgacgccc aagatcgagg
     3541 gcaagttctc gctgcgcgcg tcgatcaccg gcgagatcgt catggaggac gtgttcgtgc
     3601 ccgaggagaa tctgctgccc ggcgtctcgg ggctcaaagg gccgttctcc tgcctcaaca
     3661 acgcgcgcta cggcatcgcc tggggagcgc tcggcgctgc cgagttctgc tggcacgcgg
     3721 cgcgcgacta cacgctctcg cgcaccgctt tcggcaagcc gctcgccgcg aaccagctca
     3781 tccagaagaa actcgcggac atgcagaccg agatcaccat cggactccac gcctgcctgc
     3841 gcctgggccg gctcatggac gagcatcgcg cgacgcccga gatggtatcg atgctgaagc
     3901 gcaattcctg cggcaaggcg ctcgacatcg cccgcatggc gcgcgacatg cacggcggca
     3961 acggcatcgt cgacgaatat cacgtgatcc gccacgtgct gaatctcgag accgtcaaca
     4021 cctatgaggg cacgcacgac atccacgccc tcattctcgg acgcgcgcaa accggcctcg
     4081 ccgcgtttta gcgacgctcc gatcggagcg gaatccttgt ttcatcgccc cctctttcgc
     4141 ggggacgacg atggaagaat atcccgctcc tgcttggtcg atcgcaccgc ttgcgtcgcg
     4201 ttcctgctgc tcgcacttgc actctcagcg atgccggagc gcgcgaccgc cggaccgccc
     4261 tatctcaccg acgatccgga ccccgtcgac tacggtcact gggaggccta ccttttctcc
     4321 accggaaccg ccgtccatgg ggatgccgcg ggcgtccttc cggcagtcga ggtgaattac
     4381 ggcgcgctgc cgaatttgca tctgcacatc cagatccccg tcgcttacaa cagccaaagc
     4441 ctcaccggta cgcaattcgg catgggcgac gcctccttcg gcgcgaaata ccgcttcatc
     4501 accgccgaca agcaggattg gtggccggag gtcgcgacct ttcccatgat cgcggtgccg
     4561 accggcgatg aggcacggag cctcggcact ggacgcacgc atgtcttcct gccgctctgg
     4621 gcgctgaaga gcttcgggct gtggacgacc tatggcggcg gcgggtactg gatcaatcca
     4681 ggacccggca atcgcgactt ctggttcgcc ggctggctgc tgcagcgtca ggtcaccgac
     4741 gatttcgcgc tcggcgtgga gctcttccat cagtcggcat cggccacggg cggccccggc
     4801 agcgtcggct tcccgctcgg cagcaaggac accaccggat tcaatatcgg cggcatctat
     4861 gacctgagcg agcattatca tttgctgatg tcagccggtc gcggcttcca gaacgcgtcg
     4921 gactcgaacg agctctcgta ttacgttgcg ttgcagtgga cgttctgaga agaagtcgtc
     4981 agttatcagt tgtcagtggc agcgcattcg tgactgagaa ctgacaactg agaactgacg
     5041 actttaaaaa tagaaacgcc gcgctcgggg acccgagcgc ggcgtctggt ggaccccagg
     5101 aggaaaacta ggcggcgggc gggaactgta cggcgcgggc gccgagctcg acggtggtcg
     5161 tccagaagcg gtcgaggcga cgcagcgcgg cgaaagcggc cgtgagatcc tgctcggtga
     5221 gcgggccctt cgacagcgcg tcgatctgcc gttcatgcat cttgacgatc ttctggcgca
     5281 gcgcctggcc cttctcggag aggcggacgc gcacggagcg gcggtcatgg gccgagcgct
     5341 cctggacgag gtagtcggcc tcgaccatct tcttgacatt gtaggagacg ttcgagccga
     5401 ggtagcagcc gcggagcgtc aactcgccga ccgtcatctc gagatcgccg atgttgaaga
     5461 ggatcatggc ctgcacgttg ttgatgtcgt gcacgccctg agcatcgagc tcaagcttca
     5521 ccacctcgag atattggcgg tgggagcgct cgacgagcgc gatgatgtcg agataggcgg
     5581 tcctcatgct acccccggat cgagatggtg ctagtacggc tccggccaaa attaggggtc
     5641 ccgggttaaa aagtccttta agcgaatttg cgccgcttta gccaccaatg gtggagggtg
     5701 tgccggcgac gagcccaaag attgccgaga agtccttcgg ccccagaccg gcgttgcaaa
     5761 gcatttgata gagcgcgccc gcctcggcgc cgagcggcgt cgccgcaccc gcagcctgcg
     5821 ccgcctgctg cgccagcttc aggtccttga gcatcatgtc ggcggtgaag cccgcctcgt
     5881 aatcgcgatt ggcgggcgag gccggcaccg gtcccggcac cgggcaatag ctcgtcatcg
     5941 cccagcattg gcccgaagat ttcgagctga tgtcgaagag cttctgccgg tcgaggccga
     6001 gccggtcggc cagcgcgaag ccctcgcaca cgccgatcat cgacaccgcg agcatcatgt
     6061 tgttgcagat cttggccgcc tgaccattgc cggccggccc cgcatggacg atggtcttgc
     6121 ccatcgcctg cagcaccggc aaggcgcgct cgaagccgga cgcgctgcca ccgaccatga
     6181 atgtcagcgt ccccgccgcc gcgccggcga ctccgcccga caccggcgca tcgagcatgt
     6241 cgaagccgcg cgctgccgcc gccttgatga gcgcgcgcgc cgactcgaca tcgatggtcg
     6301 aggaatcaac gagaagggcg cccggcttcg ccgccgcaag cacgcccgcg gcaccaccat
     6361 gggccgcacg cacttgcggc cccgccggca gcatggtgag gaccatgtcg gctgccgcca
     6421 ccgtcgcggc gatgttggcc gccggcttgc cgccggcctt ggcgagcgcc gccacggcgg
     6481 gctcggcgag atcgaaaccg gtcacgtcat agccggcctt cacgagattg gccgccatcg
     6541 gcccgcccat attgccgagc ccgataaaac cgatcttcat gctcgtcgcc cctcgccgct
     6601 ttttccctct cccctgtcgg gagaaaggga ttttgcactt aaatccgatt ctgcacaaaa
     6661 acggtcaagg gtgcaccccc gcaaaagcgc cgaatgaacc ttcagtttga cgctcaggca
     6721 agagggtcct aaggttggac cctgagggga tggagttccc cataaccgcc cacgagggct
     6781 gatgactcct accgcgtcag aacgacggtg ggagtgtctc tgcgacccgc cgtgtggcgg
     6841 gttttttgtt ggccgcctca cgctcgggtt ggctttgagg agtgccgaga tgacggaaat
     6901 ctgggcgcaa gccgcattgt ggttgggcct ggcgctgatc gcgacgct
//
LOCUS       DASYNS010000303         2511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5219131, whole genome shotgun sequence.
ACCESSION   DASYNS010000303 DASYNS010000000
VERSION     DASYNS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5219131"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..523)
                     /gene="tatB"
                     /locus_tag="VGP50_09305"
     CDS             complement(110..523)
                     /gene="tatB"
                     /locus_tag="VGP50_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEV8027616.1"
                     /translation="MFDIAWSHLAVIALVALLVIGPKDLPRVLRTVGTWVGKARAVAR
                     EFQSSLDQMVREAELDEVKRQVESASSVNLERELENSIDPGGEMERSLKAPLLTAPPS
                     PLPQAEPTVARPPPAEHGPVEPPAAAEPAKSAAHD"
     gene            complement(818..1501)
                     /locus_tag="VGP50_09310"
     CDS             complement(818..1501)
                     /locus_tag="VGP50_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEV8027617.1"
                     /translation="MTPSETESDLRADQLRLLEALLFAASAPLDDESIAQRLPQGADI
                     PGLIAELGEHYRARGVNLVNVAGGWSFRTAPDLGPRLKLEQVVTRKLSRAGIETLAII
                     AYHQPVTRAEIEEIRGVVVSSGTLDVLMEAGWIAPKGRRETPGRPVTWATTDAFLSHF
                     GLADRSDLPGIDELKAAGLIGPRPDFTLSESITPLERPEEDEEESEDAEVEAEVEVEE
                     EAEGKDDGG"
     gene            complement(1498..2274)
                     /locus_tag="VGP50_09315"
     CDS             complement(1498..2274)
                     /locus_tag="VGP50_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEV8027618.1"
                     /translation="MTAPEPFDLPKPAAAAAELVVDLEGYEGPIDMLLALAREQKVDL
                     TKISILALADQYLAYIAAARSLRLEIAADYLVMAAWLAYLKSRMLLPEPETADEPSGA
                     DLAAALAHQLQRLAAMQQVGARLMARPLLGRDVFPRGAPEGLPRVLRPVWEATLYDLL
                     SAYGEQRQRKEISVLHIEAPELYSMDDALHRLERVIGRVPEWRTLASFLPAGLRGVIG
                     RSAVAATFAASLELARSGKLQLRQDTPFGPIYLRSLPETP"
     gene            complement(2271..>2511)
                     /locus_tag="VGP50_09320"
     CDS             complement(2271..>2511)
                     /locus_tag="VGP50_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV8027619.1"
                     /translation="MLPLPPLDGGRVAVGILPDALAFRLAGMERYGLVLIVGLLILMP
                     MLGQRLGMDLDVFGYLVGIPSAYAKYGILWLTGHV"
BASE COUNT          401 a          832 c          891 g          387 t
ORIGIN      
        1 catgaggagc caggcgccga tcgaataaag aagacgcgtc cgcagctcga tcagatggtc
       61 gagcagcggc atcttgcctt cttcgaggtc ctcgtctttg gccacggctt cagtcatgcg
      121 cggcggattt tgcgggctcg gcggccgccg gcggctcgac ggggccgtgc tcagccggcg
      181 gcggtctggc gacggtcggc tcggcttgcg gcaatggcga cggcggcgcc gtgagcaggg
      241 gcgccttgag gctgcgctcc atctcgccgc ccggatcgat gctgttctcg agctcgcgct
      301 cgagattgac ggagctggcc gattcgacct gccgcttcac ctcgtcgagc tcggcttcgc
      361 gcaccatctg gtcgaggctg ctctggaact cgcgggcgac ggcgcgcgcc ttgcccaccc
      421 aggtgccgac cgtgcgcagc acgcgcggca gatccttggg gccgatcacc agcagcgcca
      481 ccagcgcaat gaccgccaga tgcgaccagg cgatatcaaa catcggaacc ccgtgacctc
      541 cgccctgagc gagaggcgcg gatcatgaca aatgcccgcg ggtgccgcaa tgcgcggcgg
      601 acgcgcatgt cgtgtcggat catgtcggag tcgcgcacga atatccctct ccccttgcgg
      661 gagagggagg gacccgttgc gaagcaacgg gagggtgagg ggtgctgcgc gggatgttcg
      721 gtccagcggt atgggcggtg gacccctcac cctccccagc gctgcgcgct gggtcccctc
      781 cctctcccgc aaggggagag ggagaagatg cgcgagctta gccgccgtcg tcttttcctt
      841 cggcttcctc ttccacttcg acttcagctt cgacttccgc gtcctccgac tcctcctcgt
      901 cctcttccgg gcgctcgagc ggcgtgatcg actcggacag ggtgaaatcg gggcgcgggc
      961 cgatgaggcc ggcggctttg agctcgtcga tgccgggaag atcgctgcgg tcggcgagac
     1021 cgaaatggct gaggaaggcg tccgtcgttg cccaggtcac ggggcgcccc ggcgtctcgc
     1081 ggcggccctt gggtgcgatc cagccggcct ccatcaacac gtcgagcgtg ccggagctca
     1141 cgacgacgcc gcgaatctcc tcgatctcgg cccgcgtcac cggctggtga taggcgatga
     1201 tggcgagcgt ctcgatgccg gcgcgcgaga gcttgcgcgt caccacctgc tcgagcttga
     1261 gcctgggacc cagatcgggc gccgtgcgga agctccatcc gcccgcgaca ttgaccaggt
     1321 tgacgccgcg cgcgcggtaa tgctcgccga gctcggcgat gaggccgggg atgtcggcgc
     1381 cttgcggcag gcgctgggca atgctttcgt catcgagcgg tgcggatgcg gcgaagagca
     1441 gcgcctcgag caggcgcagc tgatcggcgc ggagatcgct ctcggtctcg gacggcgtca
     1501 tggtgtctcc ggaaggctcc gcaagtagat cggaccgaag ggcgtatcct ggcgcagctg
     1561 cagcttgccg gagcgcgcga gctcgaggct cgccgcgaaa gtcgcggcca ccgccgagcg
     1621 cccgatgacg ccgcgcaagc ctgccggcaa gaagctcgcg agcgtgcgcc actcgggaac
     1681 gcggccgatc acgcgctcga ggcgatggag cgcgtcatcc atggaataga gctcgggcgc
     1741 ctcgatatgc aggacggaga tctccttgcg ctggcgctgc tcgccatagg cgctgaggag
     1801 atcatagagc gtcgcctccc agaccgggcg cagcaccctg ggcaggcctt cgggcgcgcc
     1861 ccgcggaaag acgtcgcggc cgagaagcgg ccgcgccatg aggcgcgcgc ccacctgctg
     1921 catggcggcg aggcgctgca gctgatgcgc cagcgccgcg gcgaggtcgg cgcccgaggg
     1981 ctcgtcggcg gtttcgggct cgggcagcag catccgtgat ttgagatagg ccagccacgc
     2041 ggccatcacg agatagtcgg cggcgatctc gagccgcagg ctgcgcgccg cggcgatata
     2101 ggcgaggtac tgatcggcca gggccaggat cgagatcttg gtgagatcga ccttctgctc
     2161 gcgcgccagc gccagcagca tgtcgatcgg cccttcatag ccctcgagat cgaccaccag
     2221 ctcggcagcg gccgccgccg gcttcggcag atcgaaaggc tcgggtgctg tcatacgtgc
     2281 cccgtcagcc agaggatgcc gtatttcgca taggccgaag ggatgccgac gagatagccg
     2341 aagacatcga gatccatgcc gagacgctgg cccagcatcg gcatcaggat cagcaatccg
     2401 acgatgagca cgagcccgta gcgctccatg ccggcgaggc gaaacgccag cgcgtccggg
     2461 aggatgccga cggcgacgcg cccgccgtcg agcggcggca agggcagcat g
//
LOCUS       DASYNS010000304        14266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4751405, whole genome shotgun sequence.
ACCESSION   DASYNS010000304 DASYNS010000000
VERSION     DASYNS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 14266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14266
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4751405"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VGP50_09325"
     CDS             <1..197
                     /locus_tag="VGP50_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEV8027620.1"
                     /translation="LSHFGLADRSDLPGIDELKAAGLIGPRPDFTLAETVAPLERPEE
                     DDASEDAEVVAEAEGKDDGG"
     gene            482..895
                     /gene="tatB"
                     /locus_tag="VGP50_09330"
     CDS             482..895
                     /gene="tatB"
                     /locus_tag="VGP50_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEV8027621.1"
                     /translation="MFDIAWSHLAVIALVALLVIGPKDLPRVLRTVGTWVGKARAVAR
                     EFQSSLDQMVREAELDEVKRQVEAASSVNLERELENSIDPGGEMERSLKAPLLTAPSS
                     PLPQAEPTVAAPPPAEHGPVEPPAAAEPAKSAAHD"
     gene            888..1709
                     /gene="tatC"
                     /locus_tag="VGP50_09335"
     CDS             888..1709
                     /gene="tatC"
                     /locus_tag="VGP50_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEV8027622.1"
                     /translation="MTEGVAKDEDLEEGKMPLLDHLIELRTRLLYSIGAWLLMFAPCW
                     YLSKPIYNFLARPLADILSGGRLIYTDLTEAFFTDLRVAFWASFCLAFPMIAFQLWRF
                     VAPGLYRDERRAFFPYLFASPVLFIIGGALAYYVIFPVAFRFFASFQVAGGEGAPAIE
                     LLPKISEYLTLVMRLIFAFGITFQMPVLLTLMARVGMVTAAGLGAKRRYAWVAIAVAA
                     GVLTPPDLFSMFALMAPLVLLYEISIICCRFVEKRRAAREAADEAEPADGTSGKG"
     gene            1801..3078
                     /gene="serS"
                     /locus_tag="VGP50_09340"
     CDS             1801..3078
                     /gene="serS"
                     /locus_tag="VGP50_09340"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV8027623.1"
                     /translation="MFDLKWIREEPDAFDRGLARRGLPPQSPAILALDREWREAQTGA
                     EQLQAERNRLSKEIGVAKAQKRDAGELLQQVALGKERQTQLEARAAELRETVDTALAA
                     LPNIPADDVPDGLDETHNRLVRQHGTPPSFAFAPKEHAAIGEGLGQMDFARAGKLSGA
                     RFVVLSGALARLERALGQFMLDLHTSEFGYREVSPPLLVRDETMFGTAQLPKFAEDQF
                     HTTTGLWLIPTAEVPLTNLVAGEIIDETALPLRFTAWTPCFRAEAGAAGKDTRGMIRQ
                     HQFEKVELVSIAHPDQSAEEHERMTRCAEEVLKRLGLAYRIMLLCAGDMGFAARKTYD
                     LEVWLPGQNAYREISSCSHCGDFQARRMKARFRPKEGKGTRFVHTLNGSGLAVGRTLI
                     AVIENYQQGNGTVAVPEALRPYMGGLEVIGGDV"
     gene            3071..3853
                     /gene="surE"
                     /locus_tag="VGP50_09345"
     CDS             3071..3853
                     /gene="surE"
                     /locus_tag="VGP50_09345"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEV8027624.1"
                     /translation="MFETPIDLAAARILVSNDDGINAPGIRLLARIARSLSPDVWVVA
                     PEQEQSGASHSLTLIRPLRVRQLGRRRFAVDGTPTDCVLLAVRTILRDKPPTLVFSGV
                     NAGVNLGEDVTYSGTVAAAMEATLLGVPAIAFSQHYSDRGAIRWATAAAHAPALVRRL
                     AALPWARNTLINVNFPDVPPEEVAGIAATRQGRRKIGEGVSERLDPRGRPYFWIGPVR
                     SEWPDLPGTDLHAVANGKVSVTPIYLDLTNGPALAELKKVFP"
     gene            3859..4503
                     /locus_tag="VGP50_09350"
     CDS             3859..4503
                     /locus_tag="VGP50_09350"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEV8027625.1"
                     /translation="MATPKVLRLLMELRGAGISDARVLGAIERVPRDRFVPAAFRDQA
                     YENVALPIGHGQTISQPLVVALMTEALEVGERHKVLEIGTGSGYQAAVLARLCRRVFT
                     IERHRALLKEAEQRFAELTLRNITTRFGDGTKGWPEQAPFDRILVTAAAPDLPRKLLD
                     SLAPDGILVAPVGEERRDQQLLRIRRKDGETVTEELGGVRFVPLVEGLPRKNRE"
     gene            4672..5814
                     /locus_tag="VGP50_09355"
     CDS             4672..5814
                     /locus_tag="VGP50_09355"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEV8027626.1"
                     /translation="MRRPVAHIPLPLALALAACVRHAPPAPVMEGQSARPPPVVVASA
                     APAVVPDTPPQLAVAPLPSVARVPVEVTPLEPLPVAAPPQPRLAASSIGAGSSIPHPP
                     VPHPDRVVVQTGDTLYAIARRVDVPVRALIEANGLTAPYSVAAGRSLIVPKLRQHVVQ
                     PGETLYAVARLFGVDVATLARANDLVPPYTVRVGEVLALPEAGPVLAAAEPKPRPPAE
                     IEPAAGPQPSPPQQSQQEAVATPPSVPSRGGRSFLWPVHGRLIAGYGPGDGGTHNDGV
                     NIAAPAGTVVLAADAGTVAYAGNELRGYGNLVLIKHAGGWMTAYAHNSKLLVKRGDKV
                     KRGQPIARVGKSGTVSEPQLHFEIRRGGKALDPADYLPATPATVSG"
     gene            5874..6260
                     /locus_tag="VGP50_09360"
     CDS             5874..6260
                     /locus_tag="VGP50_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027627.1"
                     /translation="MTRRRIYLVAAGLLVAIGLGVAGFHFRGQRAEIPAEAMPFVEEG
                     PERAAFVTSAMSSCAREMVGKEALGRHLSDDEIETYCRCYANGMADRVTADDVRQMVA
                     GGEPMAILRDKAVRVLQSCGEFLPAK"
     gene            complement(6317..7186)
                     /locus_tag="VGP50_09365"
     CDS             complement(6317..7186)
                     /locus_tag="VGP50_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8027628.1"
                     /translation="MTETPKLAPLLRRVAEALERLAPPASPKNDLDGADAFVWHAERD
                     WLEPVPRVNRVEIDLLQGIDRVRDILLENTRRFAQGLPANNVLLWGARGMGKSSLVKA
                     AHAAVNAEAPRSLVLVEIHREDIPTLPRLLSLLRDTPRRCLLFCDDLSFDQQDTSYKS
                     LKAVLEGGIEGRPAHVVLYATSNRRHLLSRDMIENERSTALNPGEAVEEKVSLSDRFG
                     LWLGFHACDQETYHGMVRGYAARYGLDLAPEQLRAEASEWAITRGARSGRVAWQYIQD
                     LAGRLGKQIGGVD"
     gene            complement(7362..7748)
                     /locus_tag="VGP50_09370"
     CDS             complement(7362..7748)
                     /locus_tag="VGP50_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027629.1"
                     /translation="MADIIGKSLTTFDVAPDGTFFRMSFVDVGGRPTTVALPAECLGE
                     LVMTIPRIITEAMRARYSDSSLRLVYPLGDWQVETAANDGRLILTLKTTDGFQVAFAI
                     GRDDLQAMVDATGDPDRAIEAALPIQ"
     gene            7693..8931
                     /locus_tag="VGP50_09375"
     CDS             7693..8931
                     /locus_tag="VGP50_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027630.1"
                     /translation="MPSGATSKVVRLLPMMSATLPPVFASTARSLDDAPPPGPRARSL
                     TRPRHTLYFSKYNGPSQGPRAFARGGKRVDIGEIRQKGRNTMNNAESHPARSRFGQLL
                     GLCAFVFSLTASGLSLAAPGEIQIPGDHVFPESLTATADGTIFIGSLADGMVFRVAPG
                     AAAAEPWIKPGTNGLLSVLGVLADERSGTLWVCSTDLSGAGVVVPGEKQTALKSFDLA
                     TGSAKGSVPLPGTGSLCNDIAIGPDGSTFVTDSLHPRILRLKPGAAAFDIWVESPIFG
                     TMGPNLDGIAFAGKGRLYVNSYQGGKLFRVGVRKDGSAGKVTEIKTSAALDHPDGLRM
                     LGHNSFLMIEGAGRLDRVTVKGDRAQIEVLKAGLNAPVAVARVGTTAWALEGQLNLLF
                     DPAKKETKPEPFRAVAVSLK"
     gene            complement(8963..9571)
                     /locus_tag="VGP50_09380"
     CDS             complement(8963..9571)
                     /locus_tag="VGP50_09380"
                     /inference="COORDINATES: protein motif:HMM:NF022115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2478 domain-containing protein"
                     /protein_id="HEV8027631.1"
                     /translation="MGVRAGHEPHSARFDAESDVAAIVYGSADNPDRVLTEFAADLQM
                     SGARVAGLIQHARDRELRSAAGLSALLLPSGREIVVPHRHDPVTNACCLEPRGLARVA
                     AALASAIGAGADLLVINRYGTLERDGGGLLRAIGLALEADIPILIAVPEGNFRAWTTY
                     SAGMSVKLACQRQDLAAWWRSVATIPLDAPRARTTTLCETIK"
     gene            complement(9682..11181)
                     /locus_tag="VGP50_09385"
     CDS             complement(9682..11181)
                     /locus_tag="VGP50_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV8027632.1"
                     /translation="MDTIALDNRLGTPARAFLSGRTRLLIDGKLVDAASGKTFAVYNP
                     ATGTLLAQVAEADAEDVNRAVAAARRAFDEGPWSKMSPSDRGKLLWKLADLVEQNSDE
                     LAEIESIDNGKPVAVARMADVPLAVDMFRYMAGWATKITGSTIPLSFPGEFLSYTMRE
                     PVGVVGQIIPWNFPLLMAAWKLAPALAAGCTVVLKAAEQTPLSALRLAELVLEAGFPA
                     GVVNILAGFGETAGAALAAHDQVDKVAFTGSTEVGKLIVKAAAGNLKKVSLELGGKSP
                     AIVFPDADLERAIPGTAMGIFFNQGETCCAGSRLFAHKDVFDRVLDGVCGVAQSIKVG
                     PGLDPATQMGPLVSDEQFTRVTGYIDSGRKDGAQIVTGGTRAGNSGYFVTPTVITNTN
                     PGMKVVREEIFGPVLIAEAFDDDDLDRIAKEANDTIYGLAASVWTRDGGIAHKMARRL
                     RAGTVWVNCHNVFDAALPFGGYKQSGWGREMGEEVLHNYTEVKAVTTAL"
     gene            complement(11345..12364)
                     /locus_tag="VGP50_09390"
     CDS             complement(11345..12364)
                     /locus_tag="VGP50_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HEV8027633.1"
                     /translation="MRRIAAVVTGILLLAAPPLAARQFTDSAGRVVDVPERVAHVFPA
                     GPPAAIILYMLAPDRLIGWTRAPTAAERAFLPPAYADLPELGRLTGRGNTTSLEDVVR
                     RAPDLVLDIGSTAETYASLADRVQQQTRIPDVLIDGRLADAAATFRRVGVLLDVGARA
                     EELARYAEETLAEVRQRTDQVPQERRPKVYFARGPKGLETGAQGSINMEALDFVAARN
                     VAAENLGPGGIVTVSAEQVLAWQPEVIVTFNREFYASVGSDPLWQGVAAVRDKRVYLA
                     PDLPFGWVDAPPSANRLIGIRWLAKMLYPDLFPADLRAETRRFYTLFYHQAPTDQQLD
                     VLLGR"
     gene            12521..13849
                     /locus_tag="VGP50_09395"
     CDS             12521..13849
                     /locus_tag="VGP50_09395"
                     /inference="COORDINATES: protein motif:HMM:NF021593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HEV8027634.1"
                     /translation="MLRDDDVFAIRRAAGRLAPPAHRRPAVPGLRETAPAALGLALRQ
                     AAPGDAVPELPQETRRRRIWELDATLHCSIVGTCLSAGELRQLLARLDTAVGEPASDH
                     DLHGRGVIAARQRQGGGKQLNKALDRRHHVAINQFAKARSDTAVGALWADAVKRGDIP
                     GAYWAVLTHPATSEALRRKVFGEVHMLSHLVGAANRADIRRLHQLEQENAALQAKVER
                     QQGQLRDAVVSREAKLRELEAALSERIAADADSRPQSEIPAAALDALVAGLERRLAAE
                     AARRERLECRLEQSVAARAAEDERSRQALCERDALRVELAAAEQNLAALFADEGEGQP
                     LDLAGLSLLYVGGRPHQVSRLRSLAERASARFLHHDGGIDERSGVLPGLISQADIAFF
                     PVDCVSHEAALMVKRLCRQSGKPWIPLRSASITAFLAAVTAALGAARAAE"
BASE COUNT         2256 a         4901 c         4852 g         2257 t
ORIGIN      
        1 tcctcagcca tttcggtctc gccgaccgca gcgatcttcc cggcatcgac gagctcaaag
       61 ccgccggcct catcggcccg cgccccgatt tcaccttggc cgagacggtc gcgccgctcg
      121 agcgcccgga agaggacgac gcgtcggagg acgcggaagt cgtagctgaa gctgaaggga
      181 aagacgacgg cggctaacgc cgcgcatcgt ctccctctcc ccttgcggga gagggagggg
      241 acccagcgcg cagcgctggg gagggtgagg ggtccaccgc ccgtacctct ggaccgaaca
      301 tcccgcgcag cacccctcac cctcccgttg cttcgcaacg gctccctccc tctcccgcaa
      361 ggggagaggg atatttcgtg cgcgttcgac atagagacgc gcgcccgccg cgcattgcga
      421 caccggcggg catttgtcat gatccgcgcc tctcgctcag ggcggaggtc acggggttcc
      481 gatgtttgat atcgcctggt cgcatctggc ggtcattgcg ctggtggcgc tgctggtgat
      541 cggccccaag gatctgccgc gtgtgctgcg caccgtcggc acctgggtcg gcaaggcgcg
      601 cgccgtcgcc cgcgagttcc agagcagcct cgaccagatg gtgcgcgaag ccgagctcga
      661 tgaggtgaag cggcaggtcg aggccgcgag cagcgtcaac ctcgagcgcg agctcgagaa
      721 cagcatcgat ccgggcggcg agatggagcg cagcctcaag gcgcccctgc tcacggcgcc
      781 gtcgtcgcca ttgccgcaag ccgagccgac cgtcgccgcg ccgccgccgg ccgagcacgg
      841 ccccgtcgag ccgcccgcgg cggccgagcc cgcgaaatcc gccgcgcatg actgaaggcg
      901 tggccaagga cgaggatctc gaagaaggca agatgccgct gctcgatcat ctgatcgagc
      961 tgcggacgcg tctcctttat tcgatcggcg cctggctcct catgtttgcg ccgtgctggt
     1021 atctcagcaa gccgatctac aatttcctgg cgcggcctct ggccgacatc ctcagcggcg
     1081 ggcggctcat ctacacggat ctgaccgagg ccttcttcac cgatctgcgc gtcgccttct
     1141 gggcgtcgtt ctgcctcgcc ttcccgatga tcgctttcca gctctggcgc ttcgtggcgc
     1201 ccggcctcta ccgcgacgag cgccgcgcct tctttcccta cctcttcgcc tcgccggtcc
     1261 tcttcatcat cggcggcgcg cttgcctact acgtgatctt cccggtcgcc ttccgtttct
     1321 ttgcgagctt ccaggtcgcc ggcggcgagg gcgcgccggc catcgagctc ctgcccaaga
     1381 tcagcgagta tctgacgctg gtgatgcgcc tcatcttcgc cttcggcatc accttccaga
     1441 tgcccgtgct gctgacgctg atggcgcggg tgggcatggt gacggccgcc gggctcggcg
     1501 caaagcggcg ctatgcctgg gtggcgattg ccgtcgccgc gggcgtgctg acgccgcccg
     1561 acctcttcag catgttcgct ctcatggcgc cgctcgtgct cctctacgag atctcgatca
     1621 tctgctgccg cttcgtcgag aagcgccgcg ccgcgcgcga agctgcagac gaagcggagc
     1681 ccgccgacgg cacgtcgggg aaggggtaga cgcgaaaaca cgcgatggcc gggtgagggg
     1741 atcgcgccgc gaagagtggg atagatcaac aatgcacatt tgcttcgagc atcgccttcc
     1801 atgttcgacc tgaagtggat acgcgaggag ccggacgcgt tcgatcgcgg cttggcgcgg
     1861 cgcggcttgc cgccgcaatc gccggccatc ctcgcgctcg atcgcgaatg gcgcgaggcg
     1921 cagaccggcg ccgagcagct tcaggctgag cgcaacaggc tctcgaagga gatcggcgtc
     1981 gccaaggcgc agaagcgcga tgccggcgag ctcctgcagc aggtcgccct cggcaaggag
     2041 cgccagacgc agctcgaggc gcgggcggcg gagctgcgcg agacggtcga cacggcgctg
     2101 gcggcgctgc ccaacatccc cgccgatgac gtgcccgacg gcctcgacga gacccataac
     2161 cgcctcgtgc gccagcacgg cacgccgccc agcttcgcct tcgcgcccaa ggagcacgcg
     2221 gccatcggcg aaggcctcgg ccagatggac tttgcccgcg ccggcaagct ctcgggcgcg
     2281 cgctttgtcg tgctgtcggg cgcgctggcg cggctcgagc gcgcgctcgg gcagttcatg
     2341 ctcgatctgc acaccagcga attcggctat cgcgaggtga gcccgccgct cctggtgcgc
     2401 gacgagacca tgttcggcac cgcccagctc ccgaaattcg ccgaggacca gttccacacc
     2461 acgaccgggc tctggctcat cccgaccgcg gaagtgccgc tcaccaacct cgtcgccggc
     2521 gagatcatcg atgaaacggc cctgccgctg cgcttcacgg cctggacgcc atgcttccgc
     2581 gccgaggcgg gcgccgccgg caaagacacg cgcggcatga tccgccagca tcagttcgag
     2641 aaagtcgagc tggtgtcgat cgcgcatccg gatcagtccg ccgaagagca tgagcgcatg
     2701 acgcgctgcg ccgaggaagt gctgaagcgc ttgggcctcg cctatcgcat catgctgctc
     2761 tgcgccggcg atatgggttt cgcggcgcgc aagacctacg atctcgaggt ctggctaccg
     2821 ggacagaatg cgtatcgcga gatctcgagc tgctcccatt gcggggactt ccaggcgcgg
     2881 cgcatgaaag cgcgcttccg tccgaaggaa gggaagggca cccgtttcgt ccataccttg
     2941 aacggctcgg gtctcgcggt cgggcgcacg ctcatcgccg tgatcgagaa ttaccagcaa
     3001 ggcaacggca ccgtcgccgt gcccgaggcg ctccggcctt acatgggagg gctcgaggtg
     3061 atcggtggcg atgtttgaga cgcccatcga tctcgctgcg gcgcgaatcc tggtctccaa
     3121 cgacgacggc atcaacgctc cgggcatccg cctcctggcg cggatcgcgc gctcgctctc
     3181 gcccgatgtc tgggtcgtgg cccccgagca ggagcagagc ggtgccagcc attcgctcac
     3241 cctgatccgg ccgctgcgcg tgcggcagct cggccgccgc cgcttcgccg tcgacggcac
     3301 gccgaccgat tgcgtgctgc tggcggtgcg cacgattctc cgcgacaagc cgccgaccct
     3361 ggtgttctcg ggcgtcaatg ccggcgtcaa tctcggcgag gacgtaacct attcgggcac
     3421 tgtcgccgcg gccatggaag cgacgctgct cggcgtgccg gcgatcgcct tcagccagca
     3481 ctattccgac cgcggcgcca tacgctgggc gaccgccgcc gcccatgcgc cggcgctggt
     3541 gcgccggctg gcggcgctgc cctgggcgcg caataccctc atcaatgtga acttccccga
     3601 cgtgccgccg gaggaggtcg cgggcatcgc cgcgacgcgg cagggacggc gcaagatcgg
     3661 cgagggcgtg agcgagcgcc tcgatccgcg cgggcgccct tatttctgga tcgggccggt
     3721 gcgcagcgag tggccggacc tgcccggcac cgatctccac gccgtcgcca atggaaaggt
     3781 gtccgtcacg ccgatctatc tcgacctcac caacgggccg gcgctcgccg agctcaagaa
     3841 ggtgttcccg tgaacgaggt ggcgacgccc aaggtgctgc ggctgctgat ggagctgcgc
     3901 ggcgccggca tcagcgacgc gcgcgtgctg ggtgccatcg agcgggtgcc gcgcgaccgc
     3961 ttcgtgccgg ccgctttcag ggatcaggcc tatgagaatg tcgcgctgcc catcggccat
     4021 ggccagacca tcagccagcc cttggtggtg gcgctgatga cggaagcgct cgaggtcggc
     4081 gagcgtcaca aggtgctcga gatcggtacc ggctcgggtt atcaggccgc cgtgctggcg
     4141 cggctctgcc gccgcgtctt caccatcgag cgtcatcgcg ccctcctcaa ggaggccgag
     4201 cagcgcttcg ccgagctcac gctccgcaac atcaccaccc gcttcggcga cggcaccaag
     4261 ggctggccgg agcaagcgcc cttcgaccgc atcctggtga cggcggcggc gcccgatctg
     4321 ccaagaaagc tgctcgacag cctggccccc gacggcatcc tcgtggcgcc cgtcggcgag
     4381 gagcgccgcg accagcaatt gctgcgcatc cgccgcaagg acggcgagac cgtcaccgag
     4441 gagctgggcg gcgtccgctt cgttccgctg gtcgaaggac tgccgcgaaa gaaccgcgaa
     4501 tgattgcgag cgttcgtgct gtcgatcgaa tagactcagg cacgcaatga gtatgcccag
     4561 cgccgcgacc cccctccctg accctccccc tcaagggggg aggggacgca gtgaagctgc
     4621 cggaaaagcc acgaccccct ccccccttga gggggaggga tggggtgggg ggtgaggcgc
     4681 cccgtcgctc atatcccgct cccgctcgcc ctcgcgcttg cggcctgcgt ccgccatgcg
     4741 cccccggcgc ccgtgatgga ggggcaatcg gcgcggccgc cgcccgttgt cgtcgccagc
     4801 gcggcccccg cggtggtgcc ggacacgccg ccgcagctcg cggtggcacc tttgcccagc
     4861 gtcgcgcggg tgccggtcga ggtgacgccg ctcgagccgc tgcccgtcgc ggcgccgccg
     4921 caaccgcgcc tggccgcgtc gtcgatcggc gccggcagca gcatcccgca tccgccggtg
     4981 ccccatcccg atcgcgtcgt cgtccagacc ggcgacacgc tctatgcgat cgcccgccgc
     5041 gtcgacgtgc cggtgcgcgc gctcatcgag gccaacgggc tgacggcgcc ctacagcgtc
     5101 gccgccgggc gcagcctcat cgttcccaag ctgcgccagc atgtggtgca gccgggcgag
     5161 acgctctacg cggtcgcgcg cctcttcggg gtcgatgtcg cgaccctcgc ccgcgcgaac
     5221 gacctggtcc cgccctacac cgtgcgggtc ggcgaggtgc tcgctttgcc cgaggcgggg
     5281 ccagtgctgg ccgccgcgga gccgaagccg cggccgccgg cggagatcga gccggcggcg
     5341 ggcccgcagc cgtcgccgcc ccagcaatcc caacaagagg ccgtcgccac gccaccgtcg
     5401 gtgccgtcgc gcggcggccg cagcttcctc tggccggtcc atggccgcct catcgccggc
     5461 tacggcccgg gcgacggcgg cacgcataat gacggcgtca acatcgcggc gcccgccggc
     5521 acggtcgtgc tcgccgccga cgccggcacc gtcgcctatg ccggcaatga gctgcgcggc
     5581 tatggcaatc tcgtgctcat caagcatgcg ggcggctgga tgaccgccta cgcgcataat
     5641 tcgaagctgc tggtgaagcg cggcgacaag gtgaagcgcg gccagcccat cgcccgcgtc
     5701 ggcaagagcg gcaccgtcag cgagccgcag ctccacttcg agatccgacg cgggggcaag
     5761 gcgctcgacc ccgccgacta tctccccgcc acgcccgcga cggtgagcgg gtgagacgtc
     5821 gtcgcggcac cgacaacatc gcccgtcatc gaccgagcgc ctgagatcgt gcgatgacgc
     5881 gccgccggat ttatctcgtg gccgccggat tgctggtcgc gatcgggctc ggtgttgctg
     5941 gttttcactt ccgcgggcaa agggcggaga ttcccgccga ggccatgccc ttcgtcgagg
     6001 aaggcccgga gcgggccgcc ttcgtcacca gtgccatgtc atcttgcgcg cgggagatgg
     6061 tcggaaagga ggcccttgga cggcacctct ccgacgacga gatcgagact tactgccgct
     6121 gctacgcgaa cgggatggcc gatcgggtca cggcggatga tgtgaggcag atggtggccg
     6181 gcggcgagcc gatggccatc ctgcgcgaca aagcggtgcg cgttctccaa tcctgcggcg
     6241 agttcttgcc ggcgaaatga ggcacggagt cgtcccggcc tcgcgcctct ctcgcgcgaa
     6301 agcgagagat cctcgcctaa tccacgccgc cgatctgctt gccgaggcgc ccggccaagt
     6361 cctgaatata ttgccaggcg acgcggcccg agcgggcgcc gcgcgtgatt gcccattcgc
     6421 tggcctcggc gcgcagctgc tccggcgcga ggtccagccc atagcgcgcg gcatagccgc
     6481 gcaccatgcc gtgataggtt tcctggtcgc aggcgtggaa gcccagccac aggccgaagc
     6541 gatcggagag cgagactttc tcctcgacgg cttcgcccgg attgagcgcg gtcgagcgct
     6601 cgttctcgat catgtcgcgc gacagcagat ggcgccgatt cgaggtcgcg tagagcacga
     6661 catgggcggg ccgcccctcg atgccgcctt cgagcacggc cttgagcgac ttgtagctgg
     6721 tgtcctgctg gtcgaaggag aggtcatcgc agaataggag gcagcgccgc ggcgtatcgc
     6781 gcaacagcga cagcaggcgc ggcagcgtcg ggatgtcctc gcggtgaatc tcgaccagga
     6841 cgagcgagcg cggcgcctcg gcattgaccg ccgcatgcgc ggccttgacg agcgagctct
     6901 tgcccatgcc gcgcgcgccc cacagcagca cgttgttggc cggcaatccc tgggcgaagc
     6961 ggcgcgtgtt ctcgagcagg atgtcgcgca cgcggtcgat gccctggagg agatcgatct
     7021 cgacgcggtt cacccgcggc acgggctcga gccaatcgcg ttcggcgtgc cagacgaaag
     7081 cgtcggcgcc gtcgaggtcg ttcttcggcg acgcgggcgg cgcgaggcgc tccagcgcct
     7141 ccgcgacgcg ccgcagcagg ggtgccagct tgggggtctc cgtcatcgaa attcgctgat
     7201 cgctccgggc tcgagggtca ggaaggcggc gcgaccctac ggacctccca ccgcagcgtc
     7261 aatctcggcg gatggatcga tatcaagagc ttcggaagta ttcgcgccac aggctgggtc
     7321 gacggacgcg gtgtccacga ccatcgcgct tgcggacgca tctactggat cggcagagcg
     7381 gcttcgatgg cccggtcggg atcgcccgtc gcatcgacca tcgcttgcag atcgtcgcgc
     7441 ccgatggcga acgcgacctg gaacccgtcg gtggttttca atgtcagaat gagtcgcccg
     7501 tcattcgccg ccgtctcgac ttgccaatcg cccagcgggt agaccagtcg caaagaactg
     7561 tcgctgtacc tcgcacgcat cgcttcagtg atgatgcgcg gtatcgtcat cacgagttcg
     7621 ccgagacatt cggcagggag cgcgacggtt gtcggccggc cgccgacatc aacgaagctc
     7681 atgcgaaaga aggtgccgtc cggcgccacg tcaaaggtgg tcagactttt accgatgatg
     7741 tcggccacat tgcctccggt ttttgcgtcg acggctcgga gtctcgatga cgcgccgcca
     7801 cctggtccac gggcacggtc gttgacgcgc ccgcgtcaca cgctatattt cagcaaatat
     7861 aacggaccgt cgcaaggtcc gcgcgctttt gcacgaggcg gcaagcgcgt cgacattggg
     7921 gaaattcggc aaaaagggag gaacaccatg aacaatgcgg aaagccatcc ggcgcgctcg
     7981 cgctttggac agttgctcgg tctctgcgct tttgtatttt cgctcaccgc ttcgggcctt
     8041 tccctggcgg cgccgggcga gatccagatt cccggcgatc acgtgtttcc ggaaagcctg
     8101 accgcgacag ccgacgggac gatcttcatc ggcagccttg ccgacggcat ggtctttcgc
     8161 gtcgccccgg gcgccgctgc ggcagaaccc tggatcaagc cgggaacgaa cgggctcttg
     8221 tccgtgctgg gtgtcctcgc cgatgagcgg tcgggcacgc tctgggtctg ctcgacggat
     8281 ttgtcggggg cgggcgtcgt cgttcccgga gagaagcaga ccgcactcaa atccttcgat
     8341 ctcgcgaccg gttcagcaaa gggcagcgtc ccgctaccgg gcaccgggtc gctgtgcaac
     8401 gacatcgcca tcggccccga cggcagcacc ttcgtgaccg acagcctcca cccgcgcatc
     8461 ctccgtctca agccgggcgc cgctgcgttc gacatctggg tggagagccc gatcttcggc
     8521 accatgggtc cgaatctgga cggtatcgcg tttgccggca aaggccggct ctatgtcaat
     8581 tcctatcagg gcggaaaact gttccgcgtc ggtgtgcgaa aggacggcag cgccgggaaa
     8641 gtgacggaaa tcaagacgtc cgcggcgctc gatcatcccg acggcctgag aatgctggga
     8701 cacaacagtt tcctgatgat cgagggtgcc ggccgtcttg atcgtgtcac ggtcaagggc
     8761 gatcgagcgc agatcgaggt gctgaaagcc ggcttgaacg cgccggtggc ggtggcgcgg
     8821 gtcggaacga ccgcatgggc gctggaaggc cagctcaacc ttctcttcga tcccgcgaag
     8881 aaagagacca agccggaacc gttccgcgcg gtcgccgtat cgctgaaatg agagcacgcg
     8941 gcaccgcggc gccgcgtgcg cgctacttga tggtctcgca cagggttgtc gtgcgggctc
     9001 gcggcgcgtc gagcgggatg gtggcgaccg accgccacca cgccgcgaga tcctggcgct
     9061 ggcacgcgag cttgacgctc atgcctgcgg aatatgtcgt ccaagcgcgg aaattgcctt
     9121 ccggcacggc gatgaggatc gggatgtccg cctcgagggc caagccgatc gctcgcagca
     9181 gcccgccgcc gtcgcgttcg agcgtgccat agcggttgat cacgagcagg tcggcgcccg
     9241 cgccgatcgc cgatgctagg gcggcggcca cccgagccaa gccacgcggc tcgaggcaac
     9301 acgcattcgt caccggatcg tgccggtgcg gcacgacgat ctcgcggccg gaaggaagca
     9361 gaagagcgga taaaccggcg gcggagcgca gctctcgatc ccgggcatgt tgaatgaggc
     9421 cggcaactct cgcgccggac atctgaagat cggcggcaaa ttccgtcaga acgcggtcgg
     9481 gattgtcggc gctgccataa acgatggccg cgacatcgct ctcggcgtcg aagcgggcgg
     9541 agtggggctc atggccagcc ctcacgccca tcggcaacct ctggccatca aattaaacct
     9601 cccgcgcacg gcacggaact catacgggcg acctcggctc ggcgcaggcg gcggcgctaa
     9661 cgcgccgccg ccctctcggc ctcagagcgc cgtcgtcacc gccttcacct cggtgtagtt
     9721 gtgcagaacc tcttcgccca tttcgcggcc ccagcccgac tgcttgtagc cgccgaaggg
     9781 cagcgccgcg tcgaagacgt tgtggcagtt cacccagacg gtgccggcgc gcagacgcct
     9841 cgccatcttg tgcgcaatgc cgccgtcgcg ggtccacacg ctcgccgcga gaccgtagat
     9901 cgtgtcgttg gcttccttgg cgatacggtc gaggtcgtca tcatcgaacg cctcggcgat
     9961 caagaccggc ccgaagatct cttcgcgcac gaccttcatg ccgggattcg tgttcgtgat
    10021 gaccgtgggc gtcacgaaat agccgctgtt gcccgctctg gtgccgccgg tcacgatctg
    10081 agcgccgtct ttcctgccgg agtcgatgta cccggtgacg cgcgtgaatt gctcgtccga
    10141 caccagcggg cccatttgcg tcgccggatc gagacccggg ccgaccttga tcgactgggc
    10201 gacgccgcag acgccgtcga gcacgcgatc gaagacgtcc ttatgcgcga agaggcgcga
    10261 tccggcgcag caggtctcgc cctggttgaa gaagatcccc atcgcggtgc cgggaatggc
    10321 ccgctcgagg tcggcgtcgg ggaacacgat cgccggcgac ttgccgccga gctccaacga
    10381 caccttcttc aggttgccgg ccgccgcctt gacgatcagc ttgcccactt cggtcgagcc
    10441 ggtgaaggcg accttgtcga cctggtcatg ggcggcgagg gcggcaccgg ccgtctcgcc
    10501 aaagccggcg aggatgttga cgacgccggc ggggaaaccc gcctcgagca cgagctcggc
    10561 aagccggagc gcgctgagcg gggtctgctc cgccgccttg agcaccacgg tgcagcctgc
    10621 cgccaacgcc ggtgcgagct tccatgccgc catcagcaac ggaaagttcc acgggatgat
    10681 ctggccgacc acgcccacgg gctcgcgcat cgtgtaggag aggaactcgc ccgggaaaga
    10741 gagcgggatg gtcgagccgg tgatcttggt cgcccagccc gccatgtagc ggaacatgtc
    10801 gaccgcaagc ggaacgtccg ccatgcgtgc caccgcgacg ggcttgccgt tgtcgatcga
    10861 ctcgatctcg gccagttcgt cgctgttctg ctcgacgagg tcggcgagct tccacagcag
    10921 cttgccgcga tcggagggcg acatcttgct ccatggcccc tcgtcgaagg cgcggcgcgc
    10981 ggccgcgacc gcgcggttga cgtcctccgc atccgcttcg gccacctgcg ccaggagcgt
    11041 gcccgtcgcc gggttgtaga cggcgaaggt cttgcccgag gcggcatcga ccagcttgcc
    11101 gtcgatgagc aggcgggtgc gcccgctcag aaacgcgcgc gccggcgtgc cgagacgatt
    11161 gtcgagtgcg atcgtgtcca tgctttcctc ctcgttgttg tgattcgcgc tattcccaac
    11221 cgggaatagc gtcattcgcc gccaccgcgg ccggtacacc acgcccggct ctcttcccgg
    11281 tcgcgttata ttttgtcaga tacagcgacg ctaccagaga gccgcggccg cgacaaggtc
    11341 cggctcaccg tccgagcagg acgtcgagct gctgatccgt cggcgcctga tggtagaaga
    11401 gggtgtagaa gcgccgcgtc tccgcccgca gatccgcagg aaacaagtcg ggatagagca
    11461 tcttcgcgag ccagcggatg ccgatcaggc gattggccga gggcggcgcg tcgacccacc
    11521 cgaagggcag gtcgggcgcg aggtagacgc gcttgtcgcg gaccgccgcc accccctgcc
    11581 agagcggatc gctcccgacc gatgcgtaga actcgcggtt gaaggtgacg atgacctcgg
    11641 gctgccacgc caggacctgt tcggccgaga cggtaacgat gccgcccggg cccagatttt
    11701 cggccgcgac gttgcgtgcc gcgacgaaat cgagcgcctc catgttgatc gacccctgcg
    11761 cgccggtctc gaggcccttg ggtccccgcg caaagtaaac cttgggccgc cgctcctgcg
    11821 gtacctggtc ggtacgctgg cgcacctcgg caagcgtctc ttcggcgtag cgcgcgagct
    11881 cctcggcgcg cgcgccgaca tccagcagga caccgacgcg gcggaacgtc gccgcggcgt
    11941 cggccagccg cccgtcgatc agcacgtcgg ggatcctcgt ctgctgttgc acgcgatcgg
    12001 ccagcgaggc gtaggtctcc gccgtgctgc cgatatcgag gacgaggtca ggcgcgcggc
    12061 ggacgacgtc ttcgaggctc gtcgtgttgc cgcggccggt gaggcggccg agctcgggca
    12121 ggtcggcata ggccggcgga aggaaggcgc gctcggcggc cgtcggcgcg cgcgtccaac
    12181 cgatgaggcg atcgggcgcc agcatgtaga gaatgatcgc cgccggcggt cccgcgggga
    12241 agacgtgagc gacccgctcg gggacgtcga cgacgcggcc tgccgaatcc gtgaactgcc
    12301 gcgccgcgag cggcggggcc gccagcaaca gaatgccggt cacgacagcg gcgatccgtc
    12361 tcatcggctg gggctcccgg ctggtcgggc gtcggcgagg acgcccgcgt ggtatatcat
    12421 ggactatagc gaaacggact atagggaaaa ggtgcggccg ttgacagcgc tgcgctatat
    12481 cctgtgaaat acaacggctg acttgccgcg ggagagagtg atgctgcgcg acgatgacgt
    12541 ctttgcgatc cgccgtgccg caggcaggct cgcccccccc gcgcaccgcc ggccggccgt
    12601 gcccgggctg cgcgaaaccg cgcccgcagc gctcggactg gccctgcgac aggccgcccc
    12661 cggcgacgcg gtgccggaac tcccgcagga gacccgccgg cgcaggatct gggagctcga
    12721 cgccaccttg cattgctcga tcgtcggcac ctgcctttcc gcgggcgagc tgcggcagct
    12781 tctggcgcgg ctcgacaccg ccgtcggcga gccggcgtcg gaccacgacc tgcatggccg
    12841 cggcgtgatc gcggcgcggc agcggcaggg cgggggcaag cagctcaaca aggccctgga
    12901 ccggcgacat cacgtcgcga tcaaccagtt cgcgaaagcg cggagcgata ccgccgtcgg
    12961 ggctctctgg gccgacgccg tcaagcgcgg cgacatcccc ggcgcctatt gggcggtgct
    13021 gacgcacccg gcgaccagcg aggcactgcg gcgcaaggtc ttcggcgaag ttcacatgct
    13081 gtcgcatctg gtgggcgccg ccaaccgggc cgacatccgc cgcctccacc agctcgagca
    13141 ggagaacgcc gcgctccaag cgaaggtcga gcgccagcag ggacagctgc gcgacgccgt
    13201 cgtgtcgcgc gaggcgaagc tccgcgagct cgaggcggcg ctctcggaac ggatcgcagc
    13261 cgatgccgac agccggccgc agagcgagat accggccgcg gcgctcgatg ccctcgtcgc
    13321 cgggctcgag cggcggctgg cggcggaggc cgcgcgccgc gagcggctcg aatgccgcct
    13381 ggagcagagc gtggcggcgc gcgcggcgga ggacgagaga agccggcaag ccttgtgcga
    13441 gcgcgatgcg cttcgcgtcg agctggcggc ggccgagcaa aaccttgccg cgttgtttgc
    13501 cgacgaaggg gaggggcagc cgctcgatct cgccggcctc tcgcttctct acgtcggcgg
    13561 cagaccgcat caggtgagcc gcctccgctc gctcgcggag cgcgcttccg cgcggttcct
    13621 gcatcatgac ggcggcatcg acgagcgcag cggcgtattg cccgggctga tcagccaggc
    13681 cgatatcgcg ttcttcccgg tcgactgcgt cagccacgaa gcggcactga tggtgaagcg
    13741 cctctgccgc cagtcgggca aaccgtggat tcccctgcgc agtgccagca ttacggcctt
    13801 tctggcggcc gtcaccgcgg cgttgggcgc cgcgcgggcg gcggaatgag ggtcggcccc
    13861 ggcgcgggct ccggcactca ttcgtggttg atgcggccca tcggcggtta gaaatcgccg
    13921 gacgctgatc gccccacagc cccggcttga gacgctgcaa tggccgcgcg tgctcttgat
    13981 cggggtgctg cttagaaatt gataatgtgg gtcgctccgc ccgacatggc cgaacgacga
    14041 acgtccggcg cgcgaaggcc aggagaccgt ctgttgcgcg agccgctgga aacgaattga
    14101 caagcgatat atcggtatgg gtataacgaa cagagtattt ttggggggaa ctcgtgagca
    14161 agctgagcaa gtacgttttt gcgggattct cgatttgtgc cgcgctctgg tgtctgtcaa
    14221 ttcccgcgca cgcgcaagac cgcttcgacc agctctccca tcccga
//
LOCUS       DASYNS010000305         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3193546, whole genome shotgun sequence.
ACCESSION   DASYNS010000305 DASYNS010000000
VERSION     DASYNS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3193546"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="VGP50_09400"
     CDS             <1..992
                     /locus_tag="VGP50_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV8027635.1"
                     /translation="HHATPRTVGEADAALYLALTGTRFAVNSADTFAQSIGLPRAPLD
                     DFLAFHVVFGKTVPDVSINAVANLGYASGIFGTPLYPGDTVTARSEVIGLRENSNRQT
                     GSVYVRSQGFNQHGAVVLDYVRWVMVRKRDPAAPAPPPSVPALPDAVSPEALVLPAGL
                     ALARYDRALAGGQSAWNDYAVGERIDHVDGMTLEEAEHAMATRLYQNTAKVHFNLHSE
                     SKGRFGRRLVYGGHVISLARALSFNGLANAFRIAALNAGRHVAPCFAGDTIYAWSEVL
                     AKAEIAGRRDIGALRLRSIATKDQPCAEFPGKSGDVYHPSVVLDLDYWVLMPR"
     gene            1675..2064
                     /gene="sdhC"
                     /locus_tag="VGP50_09405"
     CDS             1675..2064
                     /gene="sdhC"
                     /locus_tag="VGP50_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEV8027636.1"
                     /translation="MTPAERPLSPHLQIYKPQLTSMLSIGHRAAGVALALGTPFLVWW
                     LIAAASAGDAFRLAQSFFGSWLGLVLLFGWSFAFFYHLCNGIRHLMWDTGHGLDLRST
                     YVTGWMVLAASGGLTLIAWIAGLAARG"
     gene            2069..2455
                     /gene="sdhD"
                     /locus_tag="VGP50_09410"
     CDS             2069..2455
                     /gene="sdhD"
                     /locus_tag="VGP50_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEV8027637.1"
                     /translation="MKRELRSPFARAVGLGSAKEGVDHWWAQRVSSVALVLLGLWFVA
                     SLVALAGADRARVVAWLHRPLAALLLMLTLATAFYHSALGLQVVIEDYAHSEWVKIPA
                     IVAMRLLCLALGVAGIFAVLRISFGG"
     gene            2457..>3046
                     /locus_tag="VGP50_09415"
     CDS             2457..>3046
                     /locus_tag="VGP50_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV8027638.1"
                     /translation="MPQAYPITDHEYDVVVVGGGGSGLRATLGMGAAGLRTACVSKVF
                     PTRSHTVAAQGGIGAALGNMGEDDWRWHMYDTVKGSDWLGDQDAIEYMCRQGIPAIIE
                     LEHFGVPFSRLENGKIYQRAFGGMTTHYGKGIAQRTAAAADRTGHAIIHTLYQQCLKH
                     NAEFFVEYVALDLIMDEDGACRGVLAWNLEDGTLHR"
BASE COUNT          455 a         1079 c          970 g          542 t
ORIGIN      
        1 tgcatcacgc gacgccgcgc accgtgggcg aggccgacgc cgcgctctat ctggcgctca
       61 ccggcacgcg ctttgccgtc aattccgccg ataccttcgc gcaatccatc ggcctgccgc
      121 gcgcaccgct cgacgatttc ctcgcctttc atgtcgtctt cggcaagacc gtgccggatg
      181 tgtcgatcaa cgccgtcgcc aatctcggct acgcctcggg catcttcggc actccgctct
      241 atccgggcga cacggtcacg gcgcgctcgg aggtgatcgg cttgcgcgag aactcgaacc
      301 gccagaccgg cagcgtctat gtgcgctcgc aaggcttcaa tcagcacggc gcagtggtgc
      361 tcgactatgt ccgctgggtg atggtgcgga agcgcgaccc ggcggcgccg gcaccgccgc
      421 ccagcgtgcc ggcgctgccc gacgcggtgt cgcccgaagc cctcgtcttg ccggccggac
      481 tcgcgcttgc gcgctatgat cgcgcgctgg ccggcgggca gtccgcctgg aacgactacg
      541 cggtcggcga gcgcatcgat catgtcgacg gcatgaccct cgaagaagcc gagcatgcca
      601 tggcgacgcg gctctatcag aacaccgcca aggttcattt caatcttcac agcgagagca
      661 agggtcgctt cggccgccgc ctcgtctatg gcggacacgt catcagcctg gcgcgcgcgt
      721 tgagcttcaa cgggctggcc aatgccttcc gcatcgccgc gctcaatgcc ggccgccatg
      781 tcgcgccgtg cttcgccggc gacacgatct acgcctggag cgaagtgctt gccaaagcgg
      841 agatcgcggg ccggcgcgac atcggcgcgc tgcggctgcg cagcatcgcg acgaaggacc
      901 agccttgcgc ggaatttccc ggcaagagcg gcgacgtcta tcatccgtcg gtggtgctcg
      961 atctcgatta ctgggtgctc atgccgcgct gagccgttcg ccatcaaagg cgaggggcgt
     1021 cgaagagcct cgcagctcct cgacgcccct ctggaagccg ctgccggccc cctggctcaa
     1081 cgagcggatg ggctctcccc accgcttacg gcgtccgggc gggacaccgc cttcttcgcg
     1141 ttggtcccgg tgacgggcac caaccagcgc gcacccccag catccccacc gaccgcgacc
     1201 gaagctcagt ccttgcgaac cgcttccggc ctggcgccta ggcgaccacc cgaaggcagt
     1261 cgacgtcgcc caaagatcag ggcggagcct gcgagcgccc gccgcgatgc agctgccgct
     1321 gtgacacgcc aaccgcacca ctcggagaac ccgcctgagg cgggattgcg ccggtcactg
     1381 cactctccaa ccatccaccc accgccagat gggcatccga tcctcgatgc aagggcccgg
     1441 cgctctcgcg agccatgcgc agatgattga tcagaaatgc ctgggccaca agcacttctg
     1501 tgtcgcgcgc cgaagttttc tgtgcataaa gttgcacgat ccgcacagat tgtgcgccga
     1561 tcgtccccag gtttttccac aagccgccgc gcgacgttga ctcgtggaaa tgcgcgggct
     1621 aaatcatcgc cctgtgattc cgcgcgttct gcgcgaattg ccgaggcttt ccggatgacg
     1681 cctgccgaac ggccgctctc accgcatctg caaatctaca agccgcagct gacctcgatg
     1741 ctctcgatcg gtcatcgcgc ggccggcgtc gctctcgcct tgggcacgcc gtttctcgtc
     1801 tggtggctga tcgcggcagc gagcgcgggc gatgccttcc ggctggcgca gtccttcttc
     1861 ggctcgtggc tcgggctggt gttgttgttc ggctggagct tcgcgttctt ctatcatctc
     1921 tgcaacggca tccggcacct catgtgggac acgggccacg gcctcgacct gcgcagcact
     1981 tacgtcaccg gctggatggt gctggcggcg agcggcggct tgacgctgat cgcctggatc
     2041 gccggcctcg cggcccgggg ttagcgcgat gaagcgggag ctgcgctcgc cctttgcccg
     2101 cgccgtcggc ctcggctcgg caaaggaagg agtcgaccat tggtgggcgc agcgcgtgag
     2161 ctcggtcgcg ctggtcctgc tcggcctctg gttcgtcgcg tcgctggtgg cgctcgccgg
     2221 cgccgaccgc gcgcgcgtcg tcgcctggct gcatcggccg ctggccgccc tccttctcat
     2281 gctgaccttg gcgacggcct tctatcacag cgcgctcggc cttcaggtcg tgatcgagga
     2341 ctatgcccat tccgagtggg tgaagattcc cgccatcgtc gccatgcggc tcttgtgcct
     2401 ggcgctcggc gtggccggca ttttcgccgt gctgcgcatc agctttggag gttgagatgc
     2461 ctcaggccta tccgatcacc gatcacgaat acgatgtcgt cgtcgtcggc ggcggcggct
     2521 cgggtctgcg cgcgaccctc ggcatgggcg cggccggtct ccgcaccgcc tgcgtcagca
     2581 aggtgttccc gacgcgcagc cataccgtgg cggcgcaggg cggcatcggc gcggcgctcg
     2641 gcaacatggg cgaggacgac tggcgctggc acatgtacga cacggtcaag ggctccgact
     2701 ggctcggcga ccaggacgcg atcgagtata tgtgccgcca gggcatcccg gcgatcatcg
     2761 agctcgagca tttcggcgtg cccttctcgc gcctcgagaa cggcaagatc tatcagcgcg
     2821 ccttcggcgg catgaccacg cattacggca agggcatcgc gcagcgcacc gccgccgccg
     2881 ccgatcgcac cggccacgcc atcatccaca cgctctatca gcagtgcctg aagcacaacg
     2941 ccgagttctt cgtcgagtat gtcgcgctcg acctcatcat ggacgaggac ggcgcctgcc
     3001 gcggcgtgct cgcctggaat ctcgaggacg gcacgctcca ccgctt
//
LOCUS       DASYNS010000306        10350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4130720, whole genome shotgun sequence.
ACCESSION   DASYNS010000306 DASYNS010000000
VERSION     DASYNS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10350
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4130720"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1610
                     /locus_tag="VGP50_09420"
     CDS             <1..1610
                     /locus_tag="VGP50_09420"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEV8027639.1"
                     /translation="ASGADVFFDVTTPKFTIQAIRTLPQIGWKPLHIINSVSNSPDGV
                     LKPAGLDNAQGILSGYYIEDVANPVFKDHPGVKEWNAFAEKNLPPAVQSSPFAVYGYL
                     LAETLVEVLKQCGNDLTRDNVMRQAASLKGLKLPLLVAGVAINTSASDYAPIEQMQIA
                     RVVGERFEPFGAVLSGVDPGAVSEGLKSIFRFGSATQETATSLNKNTVAMMTGTLGGT
                     YEEIGADLATVLDNGDKLRVLPILGRGSVQGVADILYLKGVDIGIVRGDTLDYLERNG
                     YAQNIRPQFAYITKLFNEEMHIIAPKTIDSIGALDGKTVAVGLSSGGTFVTAINVFER
                     LGIRPHFLYVEQRVALDKLRKGEIDALIAVEAKPVASISDISDANLHFVPVDYSTALQ
                     ADYLPGTLTPDDYPGLIPKGGRVHTISVASVLAAYNWPAKTDHYRRLEHFVDAFFSKL
                     PELQQPPFHPKWRDVSLNAKMAGWTRFQPAEEWLQRHPVVAEPVAAESSGSERQPVAA
                     ANPAQEREALFREFLEWRKSKSTSQPQ"
     gene            complement(1632..2417)
                     /locus_tag="VGP50_09425"
     CDS             complement(1632..2417)
                     /locus_tag="VGP50_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV8027640.1"
                     /translation="MPPLPLAALEWGSGAPLLILHGVFGSGRNWATVGQHLAASRRVL
                     ALDARNHGASPWADSMSYPEMAEDVAAFIAERRLERVALMGHSMGGKTAMLHALLRPD
                     AIGRLVVVDVAPVAYRPTLQAYARAMLAIPLAGISRRAEVDQRLAAAIPDPAERAFLV
                     QNLVLEAGGARWRLNLPVIERSMPVLSSFPEPAVGAAFRGPALFVAGERSGYLRPEHH
                     PAIKRLFPNAEIVTIAGAGHWVHAERREAFLDVVAPFLAERAG"
     gene            complement(2417..3259)
                     /locus_tag="VGP50_09430"
     CDS             complement(2417..3259)
                     /locus_tag="VGP50_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV8027641.1"
                     /translation="MSSRLLRLYPAPPQEVPLAGAYLARDLPALGSAVAPFVYGDFVA
                     SLDGRIALHDPGTGTSHVPQVLRSAGDFRLLLELIAQADCLVTHGGYLRAVAEGRLDD
                     IMQVGLGEGTGDLARWRERHGLAPQPAVAVASASLDFPLPASLARQGQRVLIATGAAA
                     PAARRERFSAAGFEVIIAGADRFVEGATLTRALGERGFRSLFLLAGPRMLETMLRDGM
                     LDRLYLTLVHRLLGGEHFHSLIAGPELGPAGRLRLAALYCDAAAGTGEWFAEFDVPRE
                     NVVR"
     gene            3468..3671
                     /locus_tag="VGP50_09435"
     CDS             3468..3671
                     /locus_tag="VGP50_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027642.1"
                     /translation="MTTITAPSLRIELTADGYQVQWRVERQYGRNTVVDLGTSQSFPT
                     RSEAQLWRAAFASALADEEPAPR"
     gene            3824..4393
                     /locus_tag="VGP50_09440"
     CDS             3824..4393
                     /locus_tag="VGP50_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027643.1"
                     /translation="MMNECCAEPSLEEVLKDAATRLLMRSDGVSESDVRGLLRRVAAK
                     LSPRTSDPAASRLPGRRLRPTWLIGLLAVAFIGVPEAHQALRAPSATIRSELTAAAVE
                     LARPVAARPTEGQLSAIRAHFPARDATVDASAWPQIAVTLQHLDQGTCGEVLGAARRI
                     EGLVVVELERYRSAADCGQDNDMTWRITP"
     gene            4534..6222
                     /locus_tag="VGP50_09445"
     CDS             4534..6222
                     /locus_tag="VGP50_09445"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV8027644.1"
                     /translation="MAFTVPQFIESRRDQVFPILDASEIDRLRRFGVVRHYRPGEAVT
                     RAGEVGPGMAIMLSGHINIIRHDTAGHEAVMITFGPGALLGEFAQLAGRPSLMDASAQ
                     DAVEALVIPPEKLRALLIVEAELGERIMRAVILRRTGLIQSGGVGPVIVGGDGNADVL
                     RLENFLRRNGHPHQRLNPETDAEARALVERFQIEPGQLPIVLCAGGQVLRNPSDMELA
                     RCIGLMAPIDPNRIYDVAIVGAGPAGLATAVYAASEGLSVVVLDCRGFGGQAGASARI
                     ENYLGFPTGITGMALMARAYSQAQKFGVEMVIPCEAIALEAASAGDGGYSILRLAADE
                     RVCARSVVIASGARYRQLAVEGLGSFGGACVHYWVSPLEGKLCAKQEVALVGAGNSAG
                     QAIVYMAGQASKVWVLARGKDLGASMSRYLVDRIAGLSNVEVLTETEVTGLEGDDGML
                     EAIRWRQRGSREDVRRAIRHLFLFIGAEPNTDWLSGSGVALDAKGFVLSGSDVSPDRR
                     LLETSRRGVFAIGDVRSGSAKRVAAAVGEGAQVAALLHGTVAAVGHRSPEAITR"
     gene            complement(6248..7345)
                     /locus_tag="VGP50_09450"
     CDS             complement(6248..7345)
                     /locus_tag="VGP50_09450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV8027645.1"
                     /translation="MGQSLGKTARAADWTLLPPGESGFAADLAPQLDKAIAEKRIWNV
                     HGVVILRKGGLAFERYFDGEDNVRGQPLGTVVFKVDTLHDLRSVSKSIVGLLYGIALA
                     DHKVPPPEAPLFASFPEYADLAADPARGRWTIQHVLTMTMGTDWDELSIPYTDPANSE
                     IAMDRAPDRYRFILERPIVREPGTHWTYSGGATALLARIIAKGTGRPLHDFARERLFD
                     PLALGPTEWLADRNNEPIAASGLRMTPRDLAHIGMTTLEGGMSGGRQIVPAEWIKRST
                     TAMVDCDEIRSYGYHWYLGRFEFTVSTGPKASRARLEHYWAAIGNGGQRLFVFPGLEM
                     VVAVTAGNYDTPDQWVPPTRLVREVVLPSLS"
     gene            7558..8322
                     /locus_tag="VGP50_09455"
     CDS             7558..8322
                     /locus_tag="VGP50_09455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8027646.1"
                     /translation="MNHLDRVRAEFTRQADRFGAAAATTRRDLAQRIVDAVPAESMVL
                     DLACGPGIVSAALAAKVRNVVALDLTWRMLANARQRCAAARLANVAFVEGSADDLAFA
                     AAAFDAVVTRLSIHHFREPARVLDEVGRVLRPGGWIVLADVVSSDDPAKSALHNALEV
                     LRDPSHVRMLPATELAALIGGAGFSIEAQDDWDQPRELEEWLAIVDDPERAMPLRPIL
                     HALAAHGEDAGIGLSLDGETIRFVHRWQLIVARRPN"
     gene            8388..8852
                     /locus_tag="VGP50_09460"
     CDS             8388..8852
                     /locus_tag="VGP50_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV8027647.1"
                     /translation="MAGRVEARLKELKIDLPQAAAPAANYVPTVRSGDLLFVAGQICQ
                     WNGERRFIGKLGRDISLEQGQEAARLCALNILAQAKAALGDLDRVLRCVRLGAFINGT
                     DDFTKQPQVANGASDLFVEVLGDAGHHARAAVGVNALPGGVAVEIDAVFEVR"
     gene            8867..10042
                     /locus_tag="VGP50_09465"
     CDS             8867..10042
                     /locus_tag="VGP50_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8027648.1"
                     /translation="MADGAEHVTVKVVSRLADIPASEWDACAGDDNPFLSHAFLDALE
                     QSGSATGETGWQPQHLALEDADGRLLAAVPLYLKDHSYGEYVFDWGWASAYEGAGGRY
                     YPKLQCAVPFTPVTGRRMLLRPDAPASAGDALIAAMIELARRHKVSSLHVTFPTEVEW
                     ERLGAAGLLQRVGQQFHWQNDGYRSFDDFLEALNARKRKQIRRERRDALADGIEIETL
                     TGGALEERHWDAFYRFYRSTSDRKWGDAYLTRDFFSLIGERLAGRIVLMLAKKSGRYV
                     AGALNLLGRDTLYGRNWGCIGDFPFLHFEACYYRAIDFAIAHGLKRVEAGAQGPHKIQ
                     RGYLPMPTYSAHWIRDRGFAEAVADFLRREKRALAGEMAHLEETLSPFKHQPDQGDP"
     gene            10039..>10350
                     /locus_tag="VGP50_09470"
     CDS             10039..>10350
                     /locus_tag="VGP50_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027649.1"
                     /translation="MTLTLQPLGGALGVAAEGIDLDRPLDGATTETLRRALLDNLVLC
                     VRRQSLTPQSYLAAAIAFGRPITQLGVASRHPEIAEIMILSSEDRDAMGDGRPLVVGA
                     HW"
BASE COUNT         1705 a         3496 c         3514 g         1635 t
ORIGIN      
        1 aggcctcggg cgccgacgtc ttctttgacg tcaccacgcc gaaattcacc atccaggcca
       61 tccggacgct gccgcagatc ggctggaagc cgctgcacat catcaactcg gtgtccaact
      121 cgcctgacgg cgtgctcaag ccggccggct tggacaatgc gcaaggcatc ctgtcgggct
      181 actacatcga ggacgtcgca aatcccgtct tcaaggatca cccgggcgtc aaggaatgga
      241 acgcctttgc cgagaagaac ttgccgcccg ccgtgcaaag cagccccttc gcggtctacg
      301 gctatctgct ggccgagacc ctggtcgagg tgctgaagca atgcggcaac gacctgacgc
      361 gcgacaacgt catgcggcag gccgcgtcgc tcaaggggct gaagctcccg ctgctggtgg
      421 ccggcgttgc catcaacacc agcgccagcg attatgcgcc gatcgagcag atgcagatcg
      481 cgcgcgtcgt cggcgagcgc ttcgagccct ttggcgcggt cctgagcggc gtcgatccgg
      541 gtgcggtcag cgagggcctc aagtcgatct tccgcttcgg cagcgccacc caagagaccg
      601 cgaccagcct caacaagaat accgtcgcca tgatgaccgg gaccctcggc ggcacctatg
      661 aggagatcgg cgccgatctc gccaccgtcc tcgacaacgg cgacaagctc cgcgtgctgc
      721 cgatcctcgg ccgcgggtcc gtccagggcg tcgccgacat tctctatctg aaaggcgtcg
      781 atatcggcat cgtccgcggc gacacgctcg attatctcga gcgcaacggc tacgcccaga
      841 acatccggcc gcaattcgcc tacatcacca agctcttcaa cgaggagatg catatcatcg
      901 cgccgaagac gatcgacagc atcggcgcgc tcgacggcaa gacggtggcc gtcggcctct
      961 cgagcggcgg caccttcgtg accgccatca acgtgttcga gcggctcggc attcgcccgc
     1021 acttcctcta tgtcgagcag cgcgtagcgc tcgacaagct gcgcaagggc gagatcgatg
     1081 cgttgatcgc cgtcgaggcg aagcccgtcg cctcgatcag cgacatcagc gacgccaatc
     1141 tccatttcgt gccggtcgac tattcgacgg cgctccaggc ggactacctg ccggggacgc
     1201 tgacgccgga cgattatccg ggcctcatcc ccaagggcgg ccgcgtccac accatctcgg
     1261 ttgcctcggt gctcgcggcc tataactggc ccgccaagac cgatcactat cggcgcctcg
     1321 agcatttcgt cgatgccttc ttctcgaagc tgccggagct gcaacagccg cctttccacc
     1381 cgaaatggcg cgacgtctcg ctcaatgcga agatggcggg ctggacacgg ttccagccgg
     1441 ccgaggaatg gctgcagcgc cacccggtcg tcgccgagcc ggtggccgcg gagtcgagcg
     1501 gctccgagcg tcagcccgtc gctgcggcca acccggcgca agagcgggaa gcgctcttcc
     1561 gcgaattcct cgaatggcga aagagcaaaa gcacgagcca gccgcagtaa ctctcggtcg
     1621 gtgcgcgctg ctcaaccggc gcgttccgcc aggaagggcg cgaccacgtc gagaaacgcc
     1681 tcccggcgct cggcatgcac ccaatgcccg gcgccggcga tggtgacgat ctccgcgttg
     1741 gggaaaaggc gcttgatcgc cgggtggtgc tcgggccgca agtagcccga gcgctcgccg
     1801 gcgacgaaga gcgccggtcc ccggaaagcc gctccgaccg ccggctcggg aaagctcgaa
     1861 agaaccggca tcgagcgttc gatcaccggc agattgaggc gccagcgcgc accccccgcc
     1921 tcgagcacga gattctgaac gagaaaggcg cgctcggccg gatccgggat ggccgcggcg
     1981 agacgctgat cgacctcggc gcgccgggag atccccgcga gagggatggc gagcatggca
     2041 cgggcgtaag cctggagcgt cggccgatag gcaaccggcg ccacgtcgac caccaccagc
     2101 cgcccgatcg cgtccggccg aagaagcgcg tgcagcatcg cggtcttgcc gcccatgctg
     2161 tggcccatga gcgcaacgcg ctcaaggcgg cgctccgcga tgaaagcggc aacgtcctcg
     2221 gccatctcgg gataggacat gctgtccgcc cagggcgagg cgccgtggtt gcgcgcatcg
     2281 agcgccagca cgcggcgcga tgccgcaagg tgctgcccca cggtcgccca attgcggccc
     2341 gagccgaaga cgccgtgaag gatgagaagc ggcgcgccgc tgccccattc gagcgccgcc
     2401 agcggcaggg gcggcatcag cgcaccacat tctcgcgcgg cacgtcgaac tcggcgaacc
     2461 actcgccggt acccgccgct gcgtcgcagt agagcgccgc aaggcgcagg cgccccgccg
     2521 ggccgagctc aggaccggcg atgaggctgt ggaaatgctc gccgccgagc aggcggtgga
     2581 ccaaagtgag atagagtcga tcgagcatgc cgtcgcgcag catggtctcg agcatgcgcg
     2641 gcccggcgag caagaagagg ctgcggaagc cgcgctcgcc cagcgcgcgc gtcagcgtcg
     2701 cgccttcgac gaaacgatcc gcgccggcga tgatcacctc gaagcccgcc gcgctgaagc
     2761 gctcgcgcct ggcggccggt gccgccgcgc cggtggcgat gagcacgcgc tgcccttggc
     2821 gcgcgagcga ggccggcaaa ggaaagtcca ggctggcgct ggcgaccgcc accgccggct
     2881 gcggggcgag accgtgccgc tcgcgccagc gcgccaggtc gccggtgcct tcaccaagcc
     2941 cgacctgcat gatatcgtcg aggcggccct cggcgacggc gcggagatag ccgccatggg
     3001 tcacgaggca atcggcctgc gcgatgagct cgagcagaag gcggaaatcg ccggcgctgc
     3061 gcaggacctg cggcacatga ctcgttccgg tgccggggtc atggagcgcg atccggccat
     3121 cgaggctcgc gacgaaatcg ccataaacga agggcgcgac cgcgctgccg agcgccggaa
     3181 ggtcgcgcgc gagataagcg cccgccagcg gcacttcctg cggcggcgcg ggatagaggc
     3241 gaaggagccg cgaactcatc gcgaaatgat agcgccgccg accgggcgct gcagcagcgc
     3301 gggccaacgg gcgcgcgcgg ccgtgccgcg agcaacgtga tgaccatgct cttgatttga
     3361 gtcatggtgt gcggtgcagc aatcgcgcat ggtcgagaag tccaatgttt ccagcgggcg
     3421 ccggatcagc ctaacgaata ggcagcgcgc cgcaacaaca ggcaatcgtg acgacgataa
     3481 ccgccccatc gctcagaatc gagcttacgg ccgacggata tcaggtccaa tggcgtgtcg
     3541 aacgccagta tggccgcaac accgtggtcg atctcggcac cagccaaagc ttcccgacga
     3601 gatcggaggc gcagctctgg cgcgcggcct tcgccagtgc ccttgccgac gaagaaccag
     3661 cgccgcggtg acgcgtcagc ggggcggcct gggaaatcgc ggacgcagtc cgccgtgctt
     3721 tgatcatttt cgctgaacaa tccattcatt gcgcgaccca tagtcacagg gcggcggcga
     3781 gtgcatctta cggtagcggt cgatgccgtg atggaggccc gccatgatga acgagtgttg
     3841 cgccgagccg tcgctcgagg aagtgctgaa ggacgccgcg acgcgcctcc tgatgcgcag
     3901 cgacggagtg agcgagagcg acgttcgcgg cctgttgcgg cgcgtggccg ccaagttgtc
     3961 gccgcggacc tcggaccccg ccgcttcccg cctgcccgga cgccggctgc gcccgacatg
     4021 gctcatcggt ctcctcgccg tcgctttcat cggggttccc gaggcgcatc aggccttgcg
     4081 ggcgccaagc gcgacgatca ggagcgagct gacggcggct gcggtcgagc ttgcccggcc
     4141 ggtggccgcg cgcccgacag aggggcagct gagcgcgata cgggcgcact tcccggcccg
     4201 cgatgccacg gtcgatgcgt cggcctggcc gcagatcgcc gtcaccctcc agcatctcga
     4261 ccaaggtacc tgcggcgagg tgctgggcgc ggcgcggcgg atcgaaggcc tggtcgtcgt
     4321 ggaactcgag cgctaccgct cggccgccga ttgcggccag gacaacgaca tgacctggcg
     4381 gatcacgccg taaccccggc cgtcacacgc ctctgcttcc acgatggacg acggggcgcg
     4441 agacatggac agtcgacgcg tcggcgctaa gctgcggccg gtgactccga acgaggttgg
     4501 atcgatctcc cgcatggcaa ggggcgtgta gcgatggctt ttacggtgcc gcaattcatc
     4561 gagtcgcggc gcgaccaggt attcccgatc ctcgacgcct cggagatcga tcggctccgc
     4621 cgcttcggcg tcgtgcgcca ctaccggccg ggcgaggccg tgacgcgcgc cggcgaggtc
     4681 ggaccgggga tggcgatcat gttgtccgga cacatcaaca tcatccggca tgacaccgcg
     4741 ggccatgagg ccgtgatgat caccttcggc ccgggcgcct tgctgggcga gttcgcgcag
     4801 ctcgccgggc ggccatcgct gatggatgct tcggcgcagg acgcggtgga agcgctcgtc
     4861 atcccgccgg aaaagctccg cgctctgctg atcgtggagg cggagctcgg cgagcgcatc
     4921 atgcgggcgg tgattcttcg ccgcaccggc ctcatccagt cgggcggcgt cgggccagtc
     4981 atcgtcggcg gcgacggtaa cgccgatgtg ctgcgcctcg agaattttct ccgccgcaac
     5041 ggccatcccc atcaacggct caatcccgag accgatgcgg aagcaagggc gctggtcgag
     5101 cgcttccaga tcgagcccgg ccagctcccg atcgtgctct gcgccggtgg gcaggtgctg
     5161 cgcaatccga gcgatatgga gcttgcgcgc tgcatcggcc tcatggcgcc gatcgacccg
     5221 aaccgcatct atgacgtggc catcgtcggg gccggtccgg ccggcctcgc gacggcggtc
     5281 tatgccgcgt ccgaggggct gagcgtcgtg gtgctcgatt gccgcggctt cggcggccag
     5341 gccggcgctt cggcgcggat cgagaactac ctgggattcc cgaccggcat caccggcatg
     5401 gcgctcatgg cacgcgccta ctcccaggcg cagaaattcg gcgtcgaaat ggtgattccc
     5461 tgcgaggcga tcgcgcttga agcggcgagc gccggcgatg gcggctattc catcctcagg
     5521 ctcgccgcgg acgagcgcgt gtgtgcccgc tccgtcgtga tcgccagcgg cgcgcgctat
     5581 cgccagctcg ccgtggaggg tctcggttcc ttcggcggcg cttgcgtgca ttactgggtg
     5641 tcgccgctcg aagggaagct ctgcgccaag caggaagtcg cgctggtggg cgcgggtaat
     5701 tcggccggcc aggccatcgt ctatatggcg ggccaagcct ccaaggtatg ggtgctggcg
     5761 cgcgggaagg atctgggcgc gagcatgtcg cggtatcttg tcgaccgcat tgccggcctc
     5821 tccaatgtgg aggtgttgac ggaaaccgag gttaccggcc tcgagggcga cgatggcatg
     5881 ttggaagcga ttcgctggcg gcagcgtgga tcgcgcgagg atgttcggcg cgcgatccgg
     5941 catctcttcc tcttcatcgg cgccgagccc aatacggatt ggctgtcagg atcgggcgtc
     6001 gccctcgacg ccaagggctt cgtcttgagc ggctcggatg tgagcccgga ccgccgtctc
     6061 ctggagacca gccgacgcgg cgtgttcgcc attggcgatg tccgctcggg ctcggcgaag
     6121 cgggtggccg ccgccgtcgg cgaaggggcg caagtggcgg ccctgctgca cggcaccgtg
     6181 gccgctgtcg gccaccggtc gcccgaagcc atcacccgct gagactgccg gccgaccctc
     6241 tcacaattca cgagagggag gggagcacga cctcgcggac gagacgcgtc ggcggaaccc
     6301 attggtccgg cgtgtcgtag ttgccggcgg tgacggcgac caccatctcg agtccgggaa
     6361 agacgaagag ccgctggccg ccattgccga tcgccgccca gtagtgctcg agccgcgcgc
     6421 ggcttgcttt tggccccgtc gaaacggtga attcgaagcg gcccaaatac cagtgatagc
     6481 cgtagctgcg gatttcgtcg caatcgacca tcgccgtggt cgaccgcttg atccattcgg
     6541 ccggcacgat ctgtcgtcca ccgctcatgc cgccctccag cgtcgtcatg ccgatatgcg
     6601 cgaggtcgcg cggcgtcatg cgcaggcccg atgcggcgat gggctcgttg ttgcgatccg
     6661 ccagccactc ggtcgggccg agagccagag gatcgaagag gcgctcgcgg gcgaaatcgt
     6721 gcaaaggcct gcccgtgccc ttggcgatga tccgcgccag caaagccgtc gcgccgccgc
     6781 tataggtcca gtgcgtgccc ggctccctca cgatcggccg ctcgaggatg aaccggtagc
     6841 ggtcgggtgc cctatccatc gcgatttcgc tgttcgccgg atcggtatag gggatgctga
     6901 gctcgtccca atccgttccc atggtcatcg tcagcacatg ctggatcgtc catcggccgc
     6961 gcgcgggatc ggccgccaga tcggcgtatt ccgggaatga ggcaaagaga ggagcttccg
     7021 gcggcggcac cttgtggtcc gcgagggcga tgccgtagag caggccgacg atgcttttcg
     7081 agaccgaccg gaggtcatgc aacgtgtcga ccttgaacac gacggtgccc agaggctggc
     7141 cgcgcacatt gtcctcgccg tcgaagtaac gctcgaaggc gaggccgcct ttgcgcagga
     7201 tgaccacgcc atggacattc cagatgcgtt tctcggcaat cgccttgtcg agctgcggcg
     7261 cgagatcggc ggcgaaaccg gattcgcctg gcggaagcag agtccagtcg gcggcgcgcg
     7321 cggtctttcc cagcgattgt cccatcagca gagctccgat tcctccgagg agatcgcgcc
     7381 ggctcggtct tggcatgctc gccatcaagc ccctcccgat gtcatgattc ggacagatcg
     7441 tggttcgccg aacgtggcgg cgatgctggc atgcacgatc cgagaccgca acgggctcga
     7501 cgtgacctcg cggccgcccc gggtctataa tcgctctgcc tcgcgaggag gccagcgatg
     7561 aatcatctcg accgggtgcg cgcggaattc acgcgacagg ctgatcgctt cggcgccgcc
     7621 gccgcgacga cgcgccgcga cctggcgcaa cgcatcgtcg acgccgtccc ggctgagagc
     7681 atggtcctcg acctcgcctg cggcccgggc atcgtcagcg ccgcgcttgc cgcgaaagta
     7741 cgcaacgtcg tggccctcga cctgacgtgg cgcatgctcg cgaatgcgcg gcagcgctgc
     7801 gccgcggcga ggctcgccaa tgtcgcgttc gtcgagggaa gcgccgatga tctggccttc
     7861 gccgcggccg ctttcgacgc cgtcgtgacg cggctctcga tccatcattt ccgagagccg
     7921 gcgcgcgtcc tcgacgaagt cgggcgcgtg ctgcggcccg gcggctggat cgtcctcgcc
     7981 gatgtggtga gctccgacga tccggccaaa tcggcgctgc acaacgcgct cgaggtgctg
     8041 cgcgatcctt cccatgtgag aatgctgccc gccaccgagc tcgcggcgct gatcggcggc
     8101 gccggcttct cgatcgaagc ccaggatgac tgggaccagc cgcgcgagct ggaggaatgg
     8161 ctcgccatcg tcgatgatcc cgagcgggcg atgccgctgc gtccgattct gcatgccctc
     8221 gccgcacacg gtgaggacgc cggcatcggc ttatcgctcg atggcgaaac catccgcttc
     8281 gtccaccgct ggcagctcat cgtcgccaga cggccaaatt gagcgggtcg cctccgccgc
     8341 aggggcgggc tatgctggcc agtcactctg acggagcggg gctggccatg gcgggacgcg
     8401 tcgaagcgcg actgaaggag ctgaagatcg acttgccgca ggcggcggca ccggcggcca
     8461 attacgtccc gacggtgcgc agcggcgacc tcctgttcgt tgccggccag atctgccagt
     8521 ggaacggcga gcggcgcttc atcggcaagc tcggccgcga tatcagcctc gagcagggcc
     8581 aggaggcggc gcggctctgc gcgctcaata tcctggcgca agccaaggcc gcgctcggcg
     8641 acctcgatcg cgtgctccgt tgcgtgcgcc tcggtgcttt catcaacggc accgacgatt
     8701 tcaccaagca gccgcaagtg gcgaacggcg cctcggacct tttcgtcgag gtgctgggcg
     8761 atgccggcca ccacgcgcgc gccgccgtcg gcgtcaatgc cttgcccggg ggcgtcgccg
     8821 tcgaaatcga cgccgtgttc gaggtgcggt gaggaattga gcatcgatgg ctgacggcgc
     8881 cgagcatgtg acggtcaagg tggtctcccg gcttgccgac attccggcga gcgaatggga
     8941 cgcctgcgcc ggcgacgaca atccctttct ctcccatgcc ttcctcgacg cgctcgagca
     9001 aagcggctcg gcgaccggcg agacgggatg gcagccgcag catctggcgc tcgaggacgc
     9061 ggatggccgg cttctggccg ccgtgccgct ctatctcaag gaccattcct acggcgaata
     9121 tgtcttcgac tggggctggg catcggccta tgagggcgcc ggcggccgct attatccgaa
     9181 gctccaatgc gccgtgcctt tcacgcccgt gacggggcgg cggatgctgc tgcggcccga
     9241 cgcgcccgct agcgccggcg atgcgctgat cgcagcgatg atcgagctgg cacggcggca
     9301 caaggtgtcg tcgctccatg tcaccttccc gaccgaggtc gaatgggagc gtctcggcgc
     9361 ggcggggctg ctgcagcgcg tgggccagca attccattgg cagaatgacg gctatcggag
     9421 cttcgacgat ttcctcgagg cgctcaatgc gcggaagcgc aagcagatcc gccgcgagcg
     9481 gcgcgacgcg ctcgccgacg gcatcgagat cgagacgctg accggcggcg ccctcgagga
     9541 gcgccattgg gatgccttct accgcttcta ccgctcgacc tcggatcgca aatggggcga
     9601 tgcctatctc acccgcgact tcttcagcct gatcggcgag cgtctcgccg gacgcatcgt
     9661 gctcatgctg gcgaagaaga gcggccgcta tgtcgcaggc gcgctcaatc tcctcggccg
     9721 cgacacgctt tacggacgca actggggctg cattggcgat ttccccttct tgcatttcga
     9781 ggcctgctat taccgggcta tcgacttcgc catcgctcat ggcttgaagc gcgtcgaggc
     9841 gggagcgcaa ggaccgcaca agatccagcg cggatatctg ccgatgccga cctacagcgc
     9901 gcattggatc cgcgaccggg gcttcgccga ggcggttgcc gatttcctcc ggcgcgaaaa
     9961 acgggcgctc gccggcgaga tggcccatct cgaggagacg ctgtcgccgt tcaagcacca
    10021 gccggaccag ggagacccgt gactctgacg ctgcaacctt tgggtggtgc gctcggtgtc
    10081 gcggccgagg ggatcgacct cgaccgcccg ctcgacggcg ccacgaccga gacgctgaga
    10141 cgcgcgctcc tggacaatct cgtcttgtgc gtgcgtcgcc agtcgctgac gccgcagagc
    10201 tacctcgctg ccgcgatcgc tttcggccgc ccgatcactc agctcggcgt cgcctcgcgc
    10261 catccggaga ttgccgagat catgatcctc tcgagcgagg accgcgacgc catgggggat
    10321 ggccgcccgc tggtggtggg cgcgcattgg
//
LOCUS       DASYNS010000307         6379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4285578, whole genome shotgun sequence.
ACCESSION   DASYNS010000307 DASYNS010000000
VERSION     DASYNS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4285578"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VGP50_09475"
     CDS             <1..365
                     /locus_tag="VGP50_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEV8027650.1"
                     /translation="HTESDMASMTFGDFRAMVEDSPVPCRLLELRHEGRGLLGTCLID
                     LLDDGLSAVYSFFDPEEPRRSFGTFLIMALIAEAQRRALPYAYLGYWIAESPKMAYKA
                     RFRPLEGLGSEGWRRLAL"
     gene            453..1859
                     /locus_tag="VGP50_09480"
     CDS             453..1859
                     /locus_tag="VGP50_09480"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV8027651.1"
                     /translation="MSDRSSGTRAATARDVAALALLLAAIAALLAASGMAGEVALAKA
                     GIVVALVIASWAFGLFGEPVTTLIFFLLVVLFHVAKPETVFSGFASTAWWLVFGGSIT
                     AIAVQSTGLGRRLADLLLARVSGAYWHAVTAVAVATVALAFLMPSTNGRILLLMPIVL
                     AFAERLGLAPGRPGHTGLVLTAAAGSYMPPTTILPANVPNSILLGAADSLYGVKLTYG
                     PYFLLHFPILGALKTVLLVWLICRLCPEPQPLMLMRERDQGPPSRAERRLLMLLGLSL
                     LLFATDSLHGVSPAWVALGTGLLCLLPAVGVLSVKSFAERANIVLLLYIAGILGLGAV
                     VADSGLGARVSAWLMHLAPLAPGHTLRNVAILTAIGTGLSFLTTVTGVPAVLTPLAGQ
                     FATATGLPLLSVLMLQVVVFSTVLLPFQSPPMMVALQLGGVRLHEATRLCLALAALTI
                     LLLWPLDYLWWRLLGYLP"
     gene            1951..3639
                     /locus_tag="VGP50_09485"
     CDS             1951..3639
                     /locus_tag="VGP50_09485"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEV8027652.1"
                     /translation="MASETAVTEAGRDFIRDIIEADIKSGRHARPVTRFPPEPNGYLH
                     IGHAKSIALNFGIAQEFGGRCHLRFDDTNPAREEQEYIDAIERDVRWLGFDWGQNLHH
                     ASDYFAQLYEWAEHLIGTGKAYVDDSTPEQIRTLRGTLTEPGRDSPYRDRGAAENLNL
                     FRRMRAGEFPNGARVLRAKIDMAAPNINLRDPVLYRILHAAHPRTGTAWCIYPTYDFA
                     HGQSDAIEHVTHSICTLEFEDHRPLYDWFLDNLPVPSRPRQYEFARLNITYTVLSKRF
                     LTELVRDGRVSGWDDPRMPTLAGLRRRGVPPAALREFVGRVGVARANSVVDVQMLEHA
                     IREDLNRTAARRLAVLRPLKLVIENYPAERSEELTAANHPDDPAKGTRPLPFGRELYI
                     ERDDFMENPPKKFFRLAPGREVRLRYAYFVTCREVVKNAAGEVVELRCTYDPATRGGN
                     APDGRKVQATIHWVAAAAALPAEIRIYNPLFTRPDPGAAGDLLADLNPDSLERLGDCR
                     VEPALAASPPEGEAVQFERQGYFCRDRDAAPGRLVFNRTIGLRDSWAKAQAGKG"
     gene            3727..3978
                     /locus_tag="VGP50_09490"
     CDS             3727..3978
                     /locus_tag="VGP50_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027653.1"
                     /translation="MRLGKLGTVVVVALALGACGVTPSDRAATGAIVGGTTGALIGIA
                     FGGVGIGVGAFVGAAVGAGVGVVTNDKQVNLPPGKPVWR"
     gene            4094..4873
                     /locus_tag="VGP50_09495"
     CDS             4094..4873
                     /locus_tag="VGP50_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV8027654.1"
                     /translation="MSDDLLYELRDGIARVTFNRPQARNALTFDMYERLAAICAEVDK
                     DRSVRAMLLTGAGDKAFAAGTDISQFRAFTTPEDALAYEARIDRVIGAVEECRVPTIA
                     AIAGACTGGGAGIAAACDLRIGASNAKFGFPIARTLGNCLSMSNYARLSALIGMARAK
                     EILFTARLVEAAEAQSIGLLSEVVGEPASLMARAEDLAKRVASHAPLTLHVTKEALRR
                     LRPKIPPGEGNDLILLCYMSRDFREGMEAFLEKRAPQWRGE"
     gene            4946..5509
                     /locus_tag="VGP50_09500"
     CDS             4946..5509
                     /locus_tag="VGP50_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV8027655.1"
                     /translation="MADEAETERRWRGLMAAAQDGDRAAYERLLRETLPFLRALVAYQ
                     HRAPDRAEEVVQEVLLTVHRVRHTYDPTRPFRPWLAAIARRRSIDGLRRRTRTLAHEV
                     FDETAYETFADPEANRSMEVSAATEGLGEAIASLSPPQREAIELLKLRELSLAEAATA
                     SGRSIAALKVNVHRAIKSLRKRLAKET"
     gene            5506..6165
                     /locus_tag="VGP50_09505"
     CDS             5506..6165
                     /locus_tag="VGP50_09505"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HEV8027656.1"
                     /translation="MKSADTDKLIERLTATAGPVRRLRPPALRAALWLIAVAAVLSAA
                     ILGFADLGVFTARAADPKLALELAATLLTGILAVVAAFELGVPDRSPAWALLPLPTLA
                     LWILGSGYSCWRHLLVHGPDGWIIGDSANCFRFILGVSLPLALTLVPLLRRAAPLAPV
                     RVAALGGLGIAALSAFALQFFHPFDVTFMDLGVHAVAVALVIASLSLMERAGAARRMR
                     A"
BASE COUNT          882 a         2325 c         2097 g         1075 t
ORIGIN      
        1 gccataccga aagcgacatg gcctcgatga ccttcggcga tttccgcgcc atggtcgagg
       61 acagcccggt gccgtgccga ttgctcgagc ttcgccacga agggcgcggg ctgctcggca
      121 cctgcctcat cgacctgctc gatgacgggc tctccgccgt ctacagcttc ttcgatcccg
      181 aggagccgcg gcgcagcttc ggcaccttcc tgatcatggc gctcatcgcc gaagcgcagc
      241 gccgcgcgct cccctacgcc tatctcggct actggatcgc cgagagcccg aagatggcct
      301 acaaggcccg tttccgcccg ctcgaaggcc tcggcagcga gggctggcgc cgcctcgcgt
      361 tgtagatccc cctcccccga cgcaagatcc cgatctcgac cgcaagcccg ccgcgctgct
      421 atccctctgc cgcgggggag ggatcttcgg ccgtgagcga tcgatcgagc ggaacgcgcg
      481 ccgcgacggc gcgggacgtg gcggcgcttg ccctgctcct cgctgcgatc gccgcgttgc
      541 tcgccgcgtc cggcatggcg ggcgaggtcg cgctcgccaa ggccggcatc gtcgtcgccc
      601 tggtcatcgc ctcttgggcc ttcggcctct tcggcgagcc ggtcacgacg ttgatctttt
      661 tcctgctggt cgtgcttttt cacgtcgcga agcccgagac cgtcttttcc ggattcgcct
      721 cgaccgcgtg gtggctcgtc ttcggcggct cgatcaccgc catcgccgtt caaagcaccg
      781 gcctcggccg gcgcctcgcc gatctgctgc tggcccgggt gagcggcgcc tattggcacg
      841 cggtcacggc cgtcgccgtg gcgacggtgg ccctcgcctt tctcatgcca tcgaccaacg
      901 gacgcatcct cctgctgatg ccgatcgtcc tcgccttcgc tgagcggctg ggcctcgcgc
      961 cggggcggcc cggccatacc ggcctcgtgc tgacggccgc cgcgggcagc tacatgccgc
     1021 ccaccaccat cctgccggca aacgtgccga acagcattct gctgggtgcg gccgacagcc
     1081 tctatggcgt aaagctcacc tatgggcctt atttcctcct gcacttcccg atcctgggcg
     1141 cgctcaagac ggtgctgctg gtctggttga tctgccgtct ctgtcccgag ccgcagccgc
     1201 tcatgctgat gcgcgaacgc gaccagggtc cgccgagccg cgccgaacgc cggctcctca
     1261 tgctgctcgg cttgtcgctc ctgctgttcg ccaccgattc cctccacggt gtctcgcccg
     1321 cctgggtcgc gctcggcacg ggcctcctct gcctcctgcc ggcggtcggc gtgctctcgg
     1381 tcaagagttt cgccgagcgc gcgaacatcg tgctcctgct ctatatcgcc ggcattctcg
     1441 gcttgggcgc cgtggtcgcc gacagcggtc tcggcgcgcg cgtgagcgcg tggctcatgc
     1501 atctggcgcc gctcgcgccc gggcacacgc tccggaatgt cgccatcctg accgcgatcg
     1561 gcaccggcct ctcattcctg accacggtca ccggcgtgcc ggccgtgctg acgccgctcg
     1621 ccggccaatt cgcgacggcg accgggctgc cgctcttgag cgtcctcatg ctgcaggtgg
     1681 tcgtgttctc gacggtgctg ctgccgttcc agtcgccgcc catgatggtc gcgctgcagc
     1741 tcggcggcgt gcggctccat gaggcgacgc ggctctgcct ggcgctggcc gcgctcacca
     1801 tcctgctgct gtggccgctc gattatctgt ggtggcgatt gctgggatat ctgccgtgac
     1861 gtctcccggc gaatgcccat cttttgcgca tatgcttggc atgtcggggc ggcgtcgtta
     1921 gtctccgcct cgcttgagcg aggagcagga atggcaagcg agacggcggt gaccgaggcg
     1981 ggccgcgatt tcatccgcga catcatcgag gccgacatca agtcggggcg ccacgcccgg
     2041 ccggtgacgc gttttccgcc ggagcccaac ggctatctcc acatcggcca tgcgaagtcg
     2101 atcgccctga acttcggaat cgcgcaggaa ttcggcgggc gctgccattt gcgcttcgac
     2161 gacaccaacc cggccaggga agagcaggaa tacatcgacg ccatcgagcg cgacgtacgc
     2221 tggctgggct tcgactgggg ccagaatctc catcacgcct cggactattt cgcgcagctc
     2281 tatgagtggg ccgagcactt gatcggcacg ggcaaggcct atgtcgacga ttccacgccc
     2341 gagcagatcc gcaccctgcg cggcacgctg accgagcccg gccgcgacag cccctaccgc
     2401 gaccgcggcg ccgccgagaa tctcaatctc ttccgccgca tgcgcgcggg cgagttcccg
     2461 aacggcgcgc gcgtgctccg cgccaagatc gacatggccg ccccgaacat caatctgcgc
     2521 gatcccgtgc tctaccgcat cctccatgcg gcgcatccgc gcaccggcac cgcgtggtgc
     2581 atctatccga cctacgactt cgcgcatggg cagtcggacg cgatcgagca cgtcacccat
     2641 tcgatctgca cgctcgaatt cgaggatcat cgcccgctct atgactggtt cctcgacaac
     2701 ctgcccgtgc cgtcgcggcc ccggcaatac gagttcgcgc ggctcaacat cacctacacc
     2761 gtgctgtcga agcgcttcct caccgagctc gtgcgcgacg ggcgcgtctc cggctgggac
     2821 gatccgcgca tgccgacgct cgccggcctg cgccggcgcg gcgtgccgcc cgcggcgctc
     2881 cgcgagttcg tcggccgcgt cggcgtcgcg cgcgccaaca gcgtcgtcga cgtccagatg
     2941 ctcgagcacg cgatccgcga ggacctcaat cggaccgcgg cacggcgcct ggcggtgctc
     3001 aggccgctca agctggtgat cgagaattat ccggccgagc gcagcgaaga gctcaccgcg
     3061 gccaaccatc ccgacgatcc cgcgaaggga acgcggccgc tccccttcgg gcgcgagctc
     3121 tatatcgagc gcgacgactt catggagaac ccgccgaaga aattctttcg cctggcgccg
     3181 gggcgcgagg tgcggctgcg ctacgcctat ttcgtcacct gccgcgaggt ggtgaagaat
     3241 gcggcgggcg aggtcgtcga gctgcgctgc acctacgacc cggcgacgcg cggcggcaac
     3301 gcgcccgacg gccgcaaggt gcaggcgacg atccattggg tcgccgccgc cgccgcgctg
     3361 ccggcggaga tccgcatcta taatccgctg ttcacccgtc ccgatcccgg cgccgcgggc
     3421 gatctgctcg ccgacctcaa tccggactcg ctcgagcggc tcggcgactg ccgcgtcgag
     3481 cccgcgcttg ccgcatctcc tcccgaaggc gaggcggtgc agttcgagcg ccagggctat
     3541 ttctgccgcg accgcgacgc cgcccccggc cgcctcgtct tcaaccgcac aatcgggctc
     3601 cgcgacagct gggcaaaggc gcaggcggga aaggggtagc caggaatgcc cctgaaggac
     3661 gcttgaccga ctcgacccgc ttgtgacagt cacatcgccc ggaatgcagc attcggggga
     3721 gagaacatga ggctgggaaa actgggcacg gtcgtcgtcg tggcgttggc gctcggcgcc
     3781 tgcggcgtta cgccgagcga tcgcgccgcc accggcgcca tcgtcggcgg cacgaccggc
     3841 gcgctcatcg gcatcgcgtt cggcggcgtc gggatcggcg tgggcgcgtt cgtcggcgcc
     3901 gccgtcggcg ccggcgtcgg tgtcgtcacc aacgacaagc aggtcaacct gccgcccggc
     3961 aaacccgtct ggcgctgatg ctgctcgaat agaatggcgg cggcgcgctt cgctgcctcg
     4021 gggtcactgc ttttccggcc gctgccgcgg tgctaccgat gagcacggga tcagcaaaag
     4081 gaaagggtgc cccttgtccg acgaccttct ctacgagctt cgcgacggga tcgcgcgcgt
     4141 caccttcaat cgtccgcagg cgcggaacgc gctcaccttc gacatgtatg agcggctcgc
     4201 cgccatctgc gccgaggtcg acaaggaccg ctcggtccgc gcgatgctgc tgacgggtgc
     4261 cggcgacaag gcctttgccg ccggcaccga catctcgcag ttccgcgcct ttacgacgcc
     4321 cgaggacgcg ctcgcctatg aggcgcgcat cgaccgcgtc atcggcgccg tcgaggagtg
     4381 ccgggtcccg accatcgccg ccattgccgg cgcctgcacc ggcggcggcg ccggcatcgc
     4441 cgcggcctgc gacctgcgca tcggcgcgag caatgccaaa ttcggctttc ccatcgcgcg
     4501 cacgctcggc aactgcctct cgatgagcaa ttacgcgcgg ctttccgccc tcatcggcat
     4561 ggcgcgggcc aaggagatcc tcttcaccgc gcgcctcgtc gaggccgccg aggcgcagtc
     4621 gatcggcctc ttgagcgagg tggtgggcga gcccgcgtcg ctcatggcgc gcgccgagga
     4681 tctcgcgaag cgggtggcga gccacgcgcc gctgacgctg cacgtgacca aggaggcatt
     4741 gcggcggctg cggccgaaaa tcccgcccgg cgaaggcaat gacctcatcc tcctctgcta
     4801 catgagccgg gacttccgcg agggcatgga ggccttcctc gagaagcgcg cgccgcaatg
     4861 gcggggggag tgaccgggcg cctcgcttgt gcgccacgtc ccctctttgg ttagactggc
     4921 gaccgaggct tccgggagct ttcgcgtggc ggacgaggcg gagaccgagc ggcgctggcg
     4981 cggcctcatg gcggcggcgc aggacggcga ccgtgccgct tatgagcggc tgctgcgcga
     5041 gaccttgccc ttcctgcgcg cgctcgtcgc ttaccagcac cgcgcgccgg accgcgccga
     5101 ggaggtcgtg caggaagttc tgctgacggt gcaccgcgtg cgccatacct atgatccgac
     5161 gcgtcctttc cggccctggc tcgcggcgat cgcgcggcgg cggtcgatcg atgggctgcg
     5221 gcgccggacg cggacgctgg cgcatgaggt gttcgacgag accgcctatg aaacctttgc
     5281 cgaccccgaa gcgaacaggt ccatggaagt gtccgcggca accgaagggc tgggcgaggc
     5341 gatcgccagc ctctcgccgc cgcagcgcga ggccatcgag ctcctgaaac tgcgcgagct
     5401 cagcctggcc gaggcggcga cggcgagcgg gaggtcgatc gcggcgctca aggtcaatgt
     5461 ccaccgtgcg atcaagtcat tgaggaagcg gctcgcgaaa gaaacatgaa gagcgccgac
     5521 accgacaagc tgatcgagcg gctgaccgca acggccggcc cggtgcggcg cctgcgcccg
     5581 ccggcgctcc gcgccgcact ctggctgatc gccgtcgccg ccgtcctgtc ggcggcgatc
     5641 cttggcttcg ccgatctcgg tgtcttcacc gcccgcgccg ccgatccgaa gctcgcgctc
     5701 gagctcgccg ccacgctgct caccggcatc ctggcggtgg tcgcggcctt cgagctgggc
     5761 gtgcccgacc ggtcgccggc ctgggcgctg ctgccgctgc cgacgctggc gctgtggatc
     5821 ctcggcagcg gctacagctg ctggcggcat ctgctcgtcc atggcccgga cggctggatc
     5881 atcggcgaca gcgccaattg tttccgcttc atcctcggcg tcagccttcc gctggcgctg
     5941 accctcgtgc cgctgctgcg ccgcgcggcg ccgctcgcgc cggtgcgagt cgccgcgctc
     6001 ggcggcctcg gcatcgccgc tctgtccgcc ttcgccctcc agttcttcca tcccttcgac
     6061 gtcaccttca tggatctcgg cgtccatgcc gtcgccgtgg cgctcgtcat cgccagcctc
     6121 tccctgatgg aacgcgccgg cgcggcgcgg cgcatgcggg cttaggagta tccccggttc
     6181 gccgcggcgc ttggctgaaa cgcagggtag gggtattcgc agaggtccgc tccgcgccca
     6241 aatcggtcac cggtgcaccg cggtcgactt cccgaaagcc gacacggcgt ctcaggggcc
     6301 gtcgaccggg atgggtggaa accggccttt gccccttgca gctcagggtc tgctcttccc
     6361 ctcaggccaa tgacccaag
//
LOCUS       DASYNS010000308         5215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2102079, whole genome shotgun sequence.
ACCESSION   DASYNS010000308 DASYNS010000000
VERSION     DASYNS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102079"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VGP50_09510"
     CDS             <1..945
                     /locus_tag="VGP50_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV8027657.1"
                     /translation="LARSLEVFRDHALRLDAAERERRSAEAGLAAERHKAAIGIADAF
                     EAQVTAAVEALAAGSAEMRGVAEAMRSTAAETSQRSNVVADASAQASANVQTVATAAE
                     ELSGSVGEITRQVNHAAAIAERAVSETQKTDRMIQGLAAAAARIGEVVQLINAVASQT
                     NLLALNATIEAARAGEAGKGFAVVASEVKALANQTGKATDEIAAQITAIQSETNAAVA
                     AIKGIGATIVEINHVSTSIASAVEEQSAATREITRNTHEAAHGTGQVTSNISSVSESA
                     NKTGSLAATVLSAATGLGAQSETLRNEIDRFLKRIRAA"
     gene            1022..1510
                     /locus_tag="VGP50_09515"
     CDS             1022..1510
                     /locus_tag="VGP50_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV8027658.1"
                     /translation="MAGMTYDPERSFGFLLHDIARLLRKRFDQRARALGLSRSQWQVL
                     VHLERHEGINQSGLAEILEIENITLGRLIDRLEEAGWVERRPDRKDRRVRLLYTTKKV
                     APVMESMHKLAEETRNEALAGLAPGQRDALLDALVHVRGNLSERNGRNAEAEDDAPYA
                     ER"
     gene            complement(1546..1809)
                     /locus_tag="VGP50_09520"
     CDS             complement(1546..1809)
                     /locus_tag="VGP50_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027659.1"
                     /translation="MAVVAAASALAACAAGTDTTRGTALSVSSSDLPSIPICRDEMRA
                     YVELTRLAKLHGEGWEVFAPAVDALKQQILDCVDDKTGAFHPL"
     gene            1925..3073
                     /locus_tag="VGP50_09525"
     CDS             1925..3073
                     /locus_tag="VGP50_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8027660.1"
                     /translation="MALDSETLRQLLDTIARFVRERLVPAEASVADADKVPPALIDEM
                     RRLGLFGLTIPEGYGGLGLSMSEEVEVLFELCQTSPAFRSVHGTNNGIGSQGIVIDGT
                     EEQKRKYLPRLASGEIIASFALTEPEAGSDAASLKTSARRSGNGWVLNGTKRFITNAP
                     EASLFTVMARTDPKVAGAGGISAFLVEAPNPGIKLGAFDRKMGQRGAHTCDVIFEDCF
                     VPADALLGSREGVGFKTAMKVLDRGRLHLAAVSVGIAERLIRDMLRYAKERVQFGQPI
                     GEFQLVQAMLADSRTEAYAARSMVRDAARRKDAGEIISTEAACCKYYATEMVGRVADR
                     AVQVHGGSGYIADFGIERFYRDVRLFRLYEGTSQIQQLVIARNMLRDT"
     gene            complement(3168..3413)
                     /locus_tag="VGP50_09530"
     CDS             complement(3168..3413)
                     /locus_tag="VGP50_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027661.1"
                     /translation="MSSFEMLEAMTQDTDLRRIPVVMCSGSTRERDKERSRSLGAIGY
                     LIKPVRFEHLRPMIAKAPGTGLPQEDAAGPPVLMRKG"
     gene            complement(3586..>5215)
                     /locus_tag="VGP50_09535"
     CDS             complement(3586..>5215)
                     /locus_tag="VGP50_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027662.1"
                     /translation="GGIPLVLAPLLSPYRRHGQLSVRVERLPNGARFSKGQNNGDRTW
                     SLRPDELDDLLYQPPAGLEETHTLAVRILSLDGDHGATLAVLDLPVSPAGARAIVPPP
                     VAASVAPAGDAPVPNDAELRRLRDQLTELTARLAVREAELAQARQKLEHAQGAGSKQA
                     IEGELAAARVLWEAELKERLAAAAAEATTTLVTSRDSWQAEQGGRFAELEKRADEQIQ
                     LAQQRWQQDAKAAMSQAEASWRAGEAARLAAAEARWREEWTRALAEAEARVQRAETAL
                     VQLRPEEQIQRARDAWQQEARTALARAEESWKANEAARHAASEARWREEHRRSLAETE
                     IRAERAEAALAQARQENAAPPLPEKSAKTDEAVRLAATETRRREEVERALAETRLRAE
                     RTEAALAQAQTRSSGDAIEIHVLHDELRDVKAALALRETQLADARSAVERRGASATIS
                     KQRQLSDQLIAGLKNENRIAAPEPARAGFGRRLIGMVALLLIVTAGVMYYPQIEAAVL
                     DLWQPNAPPRASDSSAAKAKAPLHRPSLDGPRLP"
BASE COUNT          828 a         1782 c         1757 g          848 t
ORIGIN      
        1 cttgcccggt cgctcgaggt ctttcgtgac cacgcgttgc gcctcgacgc ggctgagcga
       61 gagcgccgct cggccgaggc cggcctcgcc gcggaaaggc acaaggcggc gatcggcatt
      121 gccgacgcgt tcgaggcgca ggtcaccgcg gccgtcgagg cgctcgccgc cggctcggcg
      181 gaaatgcgcg gcgtcgccga ggccatgcgc agcaccgccg cggaaacaag ccagcgctcg
      241 aacgtcgtcg cggacgcgtc ggcgcaggct tcggccaatg tgcagacggt cgccacggcc
      301 gccgaggagc tttcgggatc ggtcggcgaa attacgcgcc aggtcaatca tgccgcggcc
      361 atcgccgagc gggccgtcag cgagacacag aagacggatc gcatgatcca gggcctggcc
      421 gcggcagcgg caaggatcgg agaggtggtg cagttgatca acgccgtcgc cagccagacc
      481 aatctcttgg cgctcaatgc cacgatcgag gcggcgcgtg ccggcgaggc cgggaagggc
      541 tttgccgtcg tcgcctccga ggtgaaggcg ctggccaatc agaccggcaa agcgaccgac
      601 gagatcgccg cgcagatcac ggcgatccag tccgagacga atgccgcggt cgcggcgatc
      661 aaaggcatcg gcgcgacgat cgtcgagatc aaccatgtat cgacctccat cgcctcggct
      721 gtcgaagagc aaagcgcggc gacgcgcgag atcacgcgca acacgcacga agctgcccac
      781 ggtaccgggc aggtcacgtc caacatctcg agcgtcagcg agagcgccaa caagactggc
      841 agtctcgctg caaccgtgct gtccgcggcg accgggctcg gcgcgcagag cgagacgctg
      901 cgaaatgaga tcgaccggtt cctcaagagg atccgcgcgg cctagggccg cgtcaccaag
      961 gtgaacactt cgggttgcgc tcgtattaat aagcttgctt aacgaatgcc ggcttatcat
     1021 catggcgggc atgacatacg accccgagcg cagcttcggc ttcctcctcc acgacatcgc
     1081 gcgcctcttg cgcaagcgct tcgaccagcg ggcgcgggcg ctcggcctga gccgctcgca
     1141 atggcaggtg ctcgtccacc tcgagcgcca tgagggcatc aatcaaagcg ggctcgccga
     1201 gatcctcgag atcgaaaaca tcacgctcgg ccgtctcatc gatcgactgg aggaagcagg
     1261 ctgggtcgag cgtcgccccg accgcaagga tcgtcgcgtg cggctgctct acaccacgaa
     1321 gaaagtcgcg ccggtcatgg agagcatgca caagctcgcc gaggagacgc gcaacgaggc
     1381 gctggctggc cttgcgcccg ggcagcgcga cgcgctcctg gacgcgctcg tccatgtgcg
     1441 cggcaatctc tccgagcgca acggccggaa cgccgaggcg gaagacgacg ccccttatgc
     1501 cgagagataa cggccgccat tgatcgctga cgacggacag cgtcctcaaa gcggatggaa
     1561 cgcgccggtc ttgtcgtcga cgcaatcgag gatctgctgc ttcaaggcgt cgaccgccgg
     1621 cgcgaagacc tcccagcctt cgccgtggag cttggcgagc cgggtgagct ccacataggc
     1681 gcgcatctcg tcgcgacaga tcgggatcga cggcagatcg ctcgaggaga ccgacagcgc
     1741 cgtcccgcgc gttgtgtccg tgccggcggc gcaagccgca agcgccgacg cggcggccac
     1801 gacagccaag cggcgcaatc gcaggtctcg ggtgggcatc atgaaagata tgaacgctcg
     1861 gcgcatcatt tggttgattc tcgatccact gtcgcctagg gaatgcccct aaaggaggag
     1921 atccttggcc ctcgattccg agaccttgcg tcagttgctc gacaccatcg cccgcttcgt
     1981 tcgcgagcgc ctcgtcccgg ccgaggcgag cgtggcggac gccgacaaag tgccgcccgc
     2041 cctcatcgac gagatgcgcc ggctcggcct cttcggcctg accatccccg agggctatgg
     2101 cgggctcggc ctctccatga gcgaggaggt ggaggtcctg ttcgagctgt gccagacgtc
     2161 gccggccttc cgctcggtgc acggcaccaa caacggcatc ggcagccagg gcatcgtcat
     2221 cgacggcacc gaggagcaga agcgcaaata cctgccgcgc ctcgcctcgg gggagatcat
     2281 cgcgtccttc gccctgaccg agcccgaggc cggctcggac gccgctagcc tcaagacctc
     2341 ggcgcggcgc tccggcaatg gctgggtgtt gaacggcacc aagcgcttca tcaccaatgc
     2401 gcccgaggcg tcgctcttca ccgtgatggc gcgcaccgat ccgaaagtgg cgggtgccgg
     2461 cggaatctcc gccttcctgg tcgaggcacc taatccggga atcaagctcg gcgccttcga
     2521 tcgcaaaatg gggcagcgcg gcgcgcatac ctgcgacgtg attttcgagg actgcttcgt
     2581 gcccgccgat gcgctgctcg gcagcaggga aggggtgggc ttcaagacgg cgatgaaggt
     2641 gctcgatcgc ggccggctgc acctcgccgc ggtcagcgtc ggcatcgccg agcggctcat
     2701 ccgcgacatg ctgcgctatg ccaaggagcg cgtgcagttc ggtcagccga ttggcgagtt
     2761 ccagttggtg caggcgatgc tcgccgacag ccgcaccgag gcctatgccg cgcgctccat
     2821 ggtacgcgat gcggcgcggc gcaaggatgc aggcgagatc atctccacgg aagccgcgtg
     2881 ctgcaaatat tacgcgaccg agatggtcgg ccgcgtcgcc gatcgcgccg tgcaggtcca
     2941 tggcggctcg ggttacatcg ccgatttcgg catcgagcgc ttctatcgcg acgttcgcct
     3001 cttccgcctc tatgagggca cgagccagat ccagcagctc gtcatcgccc gcaacatgct
     3061 cagagatacg tagagccgac gctcgtcgac ggagccttgc cggaagatca ccgctttttg
     3121 cgcggacccg cgcggcgcga aggccgccac gacgccgcga ttttgcatca gcccttgcgc
     3181 atgaggacgg gcggcccggc cgcgtcctct tgggggagac cggtgccggg cgccttggcg
     3241 atcatcggtc gcagatgctc gaagcgcacc ggcttgatga gatagccaat cgcacccaga
     3301 gagcgtgacc gttccttgtc cctttcccgc gtcgagcccg agcacatcac cacggggatc
     3361 cgccgaagat cggtatcctg ggtcatcgct tccagcatct cgaagctgct cacctttctg
     3421 ccccgattga cgcacatcgc ggcagatgcg gccgcgcacg gagcaatagg tctcgccgac
     3481 cggcatgacg gcgccggcgg cggccggccg gatgatgccg ttgagcggca gccacgacac
     3541 gcgcctgccg ctcgccgcca cggcgggcgc cgcgcgctac gctaactaag gcaggcgtgg
     3601 gccatccagc gatggacggt gcagcggtgc tttcgctttc gcagcggatg agtcgctcgc
     3661 gcgcggcggg gcgttgggct gccacagatc gagaaccgcc gcctcgattt gcggatagta
     3721 catcacgccg gccgtcacga tcagaagcag ggcgaccatg ccgatgaggc ggcggccgaa
     3781 gccggcgcgc gcaggctcag gggcggcgat gcgattctcg ttcttcaggc cggcgatgag
     3841 ttggtcggac agttgccgtt gcttggagat ggtggcgctc gcgccgcgcc gttccactgc
     3901 ggatcgcgcg tccgccaatt gcgtctcgcg aagcgcaagg gcggctttca cgtcgcgcag
     3961 ctcgtcgtgc agcacgtgaa tctcgatggc atcgcccgag gatcgggtct gcgcctgcgc
     4021 cagggccgct tcggtgcgtt cggcgcgtag cctggtctcg gccagcgccc gctcgacctc
     4081 ttcgcgccgg cgtgtttcgg tcgcggcgag ccggaccgct tcgtcggttt ttgcgctctt
     4141 ctcgggcagc ggcggcgcgg cgttttcctg ccgtgcctgc gccaaggccg cttcagcccg
     4201 ctcggcgcgt atctccgtct ccgccagcga gcgtctgtgt tcttcccgcc atcgcgcttc
     4261 ggacgcggcg tgccgcgccg cctcgttggc cttccagctt tcctccgccc ttgccagcgc
     4321 ggttctcgct tcctgctgcc aagcgtcgcg cgccctttgg atttgctcct cgggccgcag
     4381 ttgcaccagt gccgtctcgg cgcgttggac gcgggcctcg gcctcggcga gggcgcgcgt
     4441 ccattcctcg cgccaccgcg cctcggccgc ggccagccgg gcggcttctc ccgcccgcca
     4501 gcttgcttcc gcctgcgaca ttgccgcctt ggcgtcttgc tgccagcgtt gttgcgccag
     4561 ctggatctgc tcgtccgccc gcttttcgag ctcggcgaag cggccaccct gttccgcctg
     4621 ccaggaatcg cggctcgtca ccagtgtcgt cgtcgcctcc gccgccgctg cggcgagccg
     4681 ttccttgagc tcggcttccc aaagaacacg agccgctgcg agctcgccct cgatcgcctg
     4741 cttcgaccct gcgccctggg cgtgttcgag tttttgccgc gcttgggcca attccgcctc
     4801 gcggaccgcg aggcgcgccg tcagctccgt cagctggtcg cggagtcggc gtagttcggc
     4861 gtcattgggg accggcgcat cgccggcagg cgccaccgac gccgccacag ggggtggtac
     4921 gattgcccgc gcaccggcgg gcgagaccgg caaatcgagc acggccaggg tggcgccgtg
     4981 gtcgccgtcc aggctcagga tccgaacggc gagggtatgc gtttcttcca agccagcggg
     5041 gggctggtag aggaggtcgt cgagctcgtc gggccgcagc gaccaggtgc gatcgccgtt
     5101 gttctgtcct ttagagaaac gggctccgtt gggcaggcgc tcgacgcgga cggaaagctg
     5161 cccgtgtcga cgatagggcg acagcagcgg agccagcacg agcggaatac cgccc
//
LOCUS       DASYNS010000309         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2415469, whole genome shotgun sequence.
ACCESSION   DASYNS010000309 DASYNS010000000
VERSION     DASYNS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2415469"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VGP50_09540"
     CDS             <1..511
                     /locus_tag="VGP50_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEV8027663.1"
                     /translation="LLKPGARIVDLGCAPGGWTQVASARVKAEAGKGRVVGIDLLATD
                     PVPGATLLREDFLAPAAPEALRAALGGAADVVLSDMAAPATGHAPTDHIRVMALAEAA
                     YDFARAVLGPGGSFVAKVLQGGSERVLLDAMKRDFTTVRHAKPPASRADSAEIYVVAQ
                     GFRGDREAQ"
     gene            641..2104
                     /gene="guaB"
                     /locus_tag="VGP50_09545"
     CDS             641..2104
                     /gene="guaB"
                     /locus_tag="VGP50_09545"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV8027664.1"
                     /translation="MEIRQALTFDDVLLVPAASSILPTETDTRTRLTREVTLDIPLIS
                     AAMDTVTESALAIAMAQAGGLGVIHRNLSIAAQADEVRKVKKFEAGMVVNPVTIGPDE
                     TLADALKLMADNRISGVPVVEPRSKRLVGIVTNRDVRFATNAKQKIAELMTKDQLVTV
                     RENVARDEAMRLLHQHRIEKLLVVDDAYRCVGLITVKDIEKSQKFPHACKDDQGRLRV
                     AAATGTGDDGVERGDALFDAGVDVLVVDTAHGHSERVLDAVRRIRRLSNYTQIVAGNV
                     ATAEGAAALIEAGADAVKVGIGPGSICTTRIVAGVGVPQLTAILDVVAACRKRDVPVI
                     ADGGIKFSGDLAKAIAAGADCAMLGSLFAGTDESPGEVFLYQGRTYKSYRGMGSVGAM
                     ARGSADRYFQQEVTSTLKLVPEGVEGRVPHKGPVHNIVHQLVGGLRAAMGYTGNRSIA
                     EMQKNCRFVQITGAGLRESHVHDIAITREAPNYRQDF"
     gene            2327..>3100
                     /locus_tag="VGP50_09550"
     CDS             2327..>3100
                     /locus_tag="VGP50_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methylase"
                     /protein_id="HEV8027665.1"
                     /translation="MQEQPLAPARDDAVRRAVTPGARIAAAIELLQAVDAGRGPADDI
                     VGEYFRRHRFAGAKDRAAISEHIYAVLRRRAALDWWIAREGSGLSADARRRMLAALVL
                     VEEWSPEAIARACDGDRFRPSPLDAAEQRLVAGVTGEKLEQRDMPPPVRGNYPEWLEP
                     HLEAVFGRALPREMAALNGAAALDLRLNRLKTNDREKLRTLLMHEGVEAARTPLSPLG
                     LRVFGRIPLGTLDVFREGLIEVQDEGSQLAALLADARPGM"
BASE COUNT          482 a         1076 c         1080 g          462 t
ORIGIN      
        1 cttgctgaag ccgggcgccc gaatcgtcga cctcggctgt gcgccgggcg gctggaccca
       61 ggtggcgagc gcccgcgtca aggcagaggc ggggaagggg cgcgtcgtcg gcatcgatct
      121 cctggccacc gaccccgtgc cgggcgccac cctgctgcgc gaggatttcc tggcgccggc
      181 cgcgcccgag gccttgcgcg cggcgctggg cggcgcggcg gatgtggtgc tgagcgacat
      241 ggcggcgcct gccaccggcc atgccccgac cgaccatatc cgcgtcatgg cgctggccga
      301 ggcagcctac gacttcgcgc gcgcggtgct ggggcccggc ggcagcttcg tcgccaaggt
      361 cctccaaggc ggcagcgagc gcgtcctgct ggacgcgatg aagcgcgact ttacgacggt
      421 ccgccatgcc aagccgccgg cgagccgcgc cgactccgcc gagatctacg tcgtggccca
      481 ggggttccgg ggagaccggg aagctcagtg atgatttccc tctccccttg cgggagaggg
      541 agggtatgcg cgtcgcgcgg cctcgggtgc ttcccttaac gcctccaatg tgtatagtgg
      601 cgcgccaacc ttcgatcccc caagggaagg gatggcacgc atggaaatcc gacaggcgct
      661 cacctttgac gacgtcctgc tggtgcctgc ggcctcgtcg atcctgccca ccgagaccga
      721 taccaggacg cgcctcaccc gcgaggtgac gctcgacatc ccgctcatct cggcggcgat
      781 ggacaccgtc accgagagcg cgctggccat cgccatggcg caagccggcg ggctcggtgt
      841 catccaccgc aacctctcga ttgcggcgca ggccgacgaa gtgcgcaagg tcaagaagtt
      901 cgaggccggc atggtggtca atcccgtcac catcggcccc gacgagacgc tggcggacgc
      961 gctcaagctg atggcggata accgcatctc cggcgttccc gtggtcgagc cgcgctccaa
     1021 gaggctcgtc ggcatcgtca ccaaccgcga cgtgcgcttt gccaccaacg ccaagcagaa
     1081 gatcgcggag ctcatgacca aggaccagct cgtcaccgtg cgcgagaacg tggcgcgcga
     1141 cgaggcgatg cggctccttc atcagcaccg catcgagaag ctgctggtgg tcgacgacgc
     1201 ctatcgctgc gtcggcctca tcaccgtcaa ggacatcgag aagtcgcaga aattcccgca
     1261 tgcctgcaag gacgaccagg ggcgcctgcg cgtcgccgcg gcgaccggca cgggcgatga
     1321 tggcgtcgag cgcggcgacg cgctcttcga tgcgggcgtc gacgtgctgg tggtggacac
     1381 ggcgcatggc cattcggagc gcgtgctcga cgccgtcagg cggatccgcc gcctcagcaa
     1441 ctacacgcag atcgtcgccg gcaatgtggc gacggccgaa ggcgccgcgg cgctcatcga
     1501 agcgggcgcg gacgccgtca aggtcggcat cggcccgggc tcgatctgca cgacgcgcat
     1561 cgtcgccggc gtcggcgtgc cgcagctcac ggcgatcctc gatgtcgtcg ccgcctgccg
     1621 caagcgtgac gtgccggtga tcgccgatgg cggcatcaaa ttctcgggcg acctcgccaa
     1681 ggcgatcgcg gccggcgccg attgcgccat gctgggctcg cttttcgccg gcaccgacga
     1741 gagcccgggc gaggtcttcc tctatcaggg ccgcacctac aaatcctacc gcggcatggg
     1801 ctcggtcggc gccatggcgc gcggctcggc cgatcgctat ttccagcagg aagtgacgag
     1861 cacgctgaag ctggtgcccg agggcgtcga aggccgtgtg ccgcacaaag ggccggtgca
     1921 caacatcgtc caccagctgg tgggcggctt gcgcgccgcc atgggctaca ccggcaaccg
     1981 cagcatcgcc gagatgcaaa agaattgccg cttcgtgcag atcaccggcg ccggtctgcg
     2041 cgaaagccac gtccacgaca tcgccatcac ccgcgaggcg ccgaattacc gccaggactt
     2101 ctaagcgccg cggcgaaagc aatcgcgaga gccgcatcgg gcggattatc aagtccttct
     2161 ccgcccttca gggcggagaa ggatttagga tgaggtgggt cgtgccgcgc gtgtcgtcgg
     2221 cgacaatcta tttgcatcat caaggaaccc ccacctcacc ccggccctct ccgccccgag
     2281 gggcggagag ggggagattt gctgtcatgg gtgcgggctc tcgcggttgc aagagcaacc
     2341 gctggcgccc gcccgcgacg acgcggtgcg tcgcgcggtg actcccgggg cgcgcatcgc
     2401 ggcggcgatc gagctgcttc aggcggtcga cgcggggagg gggcccgcgg acgacatcgt
     2461 cggcgagtat ttccgccgtc atcgcttcgc cggcgccaag gaccgcgccg ccatctccga
     2521 gcacatctat gccgtgctgc gccggcgcgc ggcgctcgac tggtggatcg cgcgcgaggg
     2581 gtccggtctg tccgccgatg cgcgccgccg gatgctggct gctctcgtgc tggtcgagga
     2641 atggtcgccc gaggcgatcg cgcgcgcctg cgacggcgac cgcttccgac cgtcgccgct
     2701 cgatgcagcg gaacagcgac tggtcgctgg ggtcaccggc gaaaagctcg agcagcgcga
     2761 catgccgccg ccggtgcgcg gcaactaccc ggagtggctc gagccgcatc tcgaggcggt
     2821 gttcggccgc gccttgccgc gggaaatggc ggcgctcaac ggcgcggccg ccctcgatct
     2881 gcgtctcaac cgcctcaaga ccaacgatcg cgagaagctg cgcacgcttc tcatgcacga
     2941 aggcgtcgag gccgcgcgaa cgccgctctc gcctctgggg cttcgcgttt tcgggcgcat
     3001 cccgctgggg acgctcgacg tcttccgcga ggggctcatc gaggtgcagg acgagggctc
     3061 gcagctcgcg gcgctgctcg ccgatgcgcg cccgggcatg
//
LOCUS       DASYNS010000310         4331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5221431, whole genome shotgun sequence.
ACCESSION   DASYNS010000310 DASYNS010000000
VERSION     DASYNS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5221431"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..944
                     /gene="trpA"
                     /locus_tag="VGP50_09555"
     CDS             57..944
                     /gene="trpA"
                     /locus_tag="VGP50_09555"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV8027666.1"
                     /translation="MSAAVGAGPEGRIARRFAALKAAGRAGLIPYFVCGDPDAETFAR
                     LLAALPEAGADLIEVGMPFSDPMADGPAIQAAGQRALRAGMTMAKTLAILRAFRARDA
                     ATPIVLMGYYNPIHRYGVARFLADAKAAGADGLIIVDMPPEHDEELCLPALAAGLDFI
                     RLATPTTDERRLPAVLAHAAGFIYYVAIAGITGTKSADAETVGNAVARLKRHSTLPVA
                     AGFGIRTPEQAAAVARIADAAAVGTALVQRVAQHVDADGKARPGLVDAVLADVRALAQ
                     GVRGARLLATAPARSGGAS"
     gene            941..1831
                     /gene="accD"
                     /locus_tag="VGP50_09560"
     CDS             941..1831
                     /gene="accD"
                     /locus_tag="VGP50_09560"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEV8027667.1"
                     /translation="MNWLTNFVLPKIRAVVAKKDVPDNLWHKCPGCGQMLFHRELEAN
                     LYVCGSCGHHLRLPPDKRLSLLFDDGRYLRIELPRTIIDPLKFRDRKRYADRLKESQA
                     KNSGSRDALVVAHGQIGGVPAVMAVFDFDFMGGSMGIAVGEGLLSAARLAVLQEAALI
                     VVPSSGGARMQEGILSLMQMPRSTLAVEMVKEAKLPYIVLLADPTTGGVSASFAMLGD
                     ITLAEPGALIGFTGARVIEETIRQKLPEGFQRAEYLLEHGMVDMVVPRRELRDTVVRI
                     IGLLRRREPNAQVVSLPARM"
     gene            1828..3135
                     /locus_tag="VGP50_09565"
     CDS             1828..3135
                     /locus_tag="VGP50_09565"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEV8027668.1"
                     /translation="MTSPLDPILRRLLQLHPKLIDLSLDRVRRLLAAIGDPQDQLPPV
                     IHVAGTNGKGSTLAFLRAALEAAGYRVNVFTKPHLVNFNERVRLNGRLIEDDALIALL
                     EECETANRGRPITYFEITTAAAFLAFARMPSDILLLETGLGGRFDATNVIAAPAVTAI
                     TPVSLDHQQFLGDTVAAIAGEKAGILKPGRPAVLAPQLPDAQAVIERRATALGAPLFR
                     YGAEWRVEPMGSGLRYEGRRWPALDLPPPGLLGRHQYDNAGTALACLDRLDGFTLSPE
                     ALARGLREVTWPARLELLAGGALAALLPAGWELWLDGGHNPAGGTVLAAMAREWEDRP
                     LHLVFGMLKGHDAAGFLRPLAPYIAGLRAVAVPGEATTRSAEEAAIVAREAGIAAVPC
                     GDVAAALKAASSSGSRGRVLICGSLYLAGHVLAENGRGTIGIG"
     gene            3302..>4331
                     /locus_tag="VGP50_09570"
     CDS             3302..>4331
                     /locus_tag="VGP50_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8027669.1"
                     /translation="MTDVALSAAAPLRRTSPAASVVALKSAVQALHHSEPHFRQLLEA
                     LPAAVYTTDAAGRITFYNEAAVALAGRRPELGTDHWCVTWRLYRPDGTPMRHDECPMA
                     IALREGRAIRGAEAVAERPDGSRVPFIPYPTPLFDDAGTLIGAVNMLVDISDRKKAEV
                     VLQQLNDTLEQRIEARTQQVRETFTKLYESERRFRLLVDGVVDYAIFMLDPDGFVTNW
                     NSGAQRIKGYSVSEIVGRHFSCFYTDEDRARGVPEAALQTASRTGRFEAEGWRRRKDG
                     TIFWANVVMDAIHDETGKLARFAKVTRDLTEKRATDEQRRQAQKMEAIGQLTGGVAHD
                     FNNLLTAII"
BASE COUNT          672 a         1528 c         1429 g          702 t
ORIGIN      
        1 tccggccgcg gcgacaagga cgtcgacagc gtcgcccagg cgctcggggt cgcgttgtga
       61 gcgccgcagt cggcgccgga ccggagggcc gcatcgcccg ccgctttgcc gcgctcaagg
      121 ccgcggggcg cgccggactc atcccctatt tcgtctgcgg cgatcccgac gccgagacct
      181 tcgcgcggct cctggcggcg ctgcccgagg ccggcgccga tctcatcgag gtcggcatgc
      241 ccttttccga tcccatggcc gacggcccgg ccatccaggc ggcaggccag cgcgcgctca
      301 gagccggcat gacaatggcg aagacgctcg ccatcctgcg cgcctttcgc gcccgcgatg
      361 cggcaacccc gatcgtgctc atggggtatt acaacccgat ccatcgctat ggcgtcgccc
      421 gttttctcgc cgacgcgaaa gcggccggcg ccgacggtct gatcatcgtc gacatgccgc
      481 ccgagcatga cgaggagctg tgcctgcccg ctcttgccgc aggcctcgat ttcattcgcc
      541 tggcgacgcc gacgaccgac gagcggcgcc tgccggcggt gctcgcccat gcggccggct
      601 tcatctatta cgtcgcgatc gccggaatca cgggcacaaa gtcggctgac gcggagaccg
      661 tggggaatgc cgtggcgcgg ctcaaacgcc actcgacctt gccggtcgcc gcgggcttcg
      721 gcattcgcac ccctgagcag gcggccgcgg tggcgcgcat cgccgacgcc gcggcggtcg
      781 gcacggcact ggtgcagcgc gtggcccagc atgtcgacgc cgacggcaag gcgcggccgg
      841 gcctcgtcga tgcggtgctc gccgatgtgc gcgcactggc ccagggcgtg cgcggcgcgc
      901 ggttgcttgc aacggcgccc gcccggagcg gaggagcctc atgaactggc tgaccaattt
      961 cgtgttgccg aaaatccgcg cggtcgtcgc caagaaggac gtgcccgaca atctctggca
     1021 caaatgtccg gggtgcggac aaatgctgtt ccaccgcgag ctcgaggcca atctctatgt
     1081 ctgcggcagc tgcggccacc atctgcgcct ccctccggat aagcgcctct ccctgctctt
     1141 cgatgatggc cgctatcttc ggatcgagct gccgcgcacc atcatcgatc ccttgaagtt
     1201 ccgcgaccgc aagcgctacg cggatcgcct caaggaatcc caggccaaga acagcgggtc
     1261 ccgcgacgcg ctcgtcgtcg cgcacggcca gatcggcggc gtgcccgccg tcatggccgt
     1321 gttcgacttc gatttcatgg gcggctcgat gggcatcgcc gtcggcgaag ggctgctctc
     1381 cgcggcccgg ctcgccgtgc ttcaggaagc ggcgctcatc gtcgtgccgt cctcgggcgg
     1441 cgcccgcatg caggaaggca tcctgtcgct gatgcagatg ccgcgcagca cgctcgcggt
     1501 cgagatggtg aaggaagcaa agcttcccta catcgtgctg ctcgccgatc cgacgaccgg
     1561 cggcgtctcg gcatctttcg ccatgcttgg cgacatcacc ctggccgagc ccggcgcgct
     1621 catcggcttc accggcgcgc gcgtcatcga ggagacgatt cgccagaagc tgccagaagg
     1681 ctttcagcgc gccgagtatc tgctcgagca tggcatggtc gacatggtcg tgccgcgtcg
     1741 cgagctgcgc gacacggtgg tacgcatcat cggcctgctg cgccgccgcg agcccaatgc
     1801 gcaggtcgtg tcgctccccg cccgaatgtg acgtcgcccc tcgatccgat cctgcggcga
     1861 ctgctgcagc tccaccccaa gctcatcgat ctgtcgctcg atcgcgtgcg ccgtttgctc
     1921 gccgcgatcg gcgatccgca ggaccagctg ccgccggtca tccatgtcgc gggcaccaac
     1981 ggcaagggct cgaccctcgc tttcctgcgg gcggcgctcg aagccgccgg atatcgcgtc
     2041 aatgtcttca ccaagccgca tctcgtgaac ttcaacgagc gtgtcaggct caacggccgg
     2101 ctcatcgagg acgacgcgct catcgcattg ctcgaggaat gcgagactgc caaccgcggc
     2161 cggcccatca cctatttcga gatcaccacg gccgcagcct ttctcgcttt tgcccgcatg
     2221 ccgtccgata tcctgctgct cgagaccggg cttggcggcc gcttcgacgc gacgaatgtc
     2281 atcgccgccc cggcggtcac cgcgattact cccgtctcgc tcgaccatca gcagttcctc
     2341 ggcgatacgg tcgcggcgat cgccggcgag aaggccggca ttctgaagcc cggccggccg
     2401 gccgtgctgg caccgcagct gcccgacgcg caagccgtga tcgagcggcg cgccacggcg
     2461 ctcggcgcgc cgctcttccg gtacggcgca gaatggcgcg tcgagccgat gggaagcggt
     2521 ttgcgctacg aaggccggcg ttggccggcg ctcgacctgc cgccgccggg cctgctcggc
     2581 cgccaccaat atgacaatgc cggcaccgca ctggcctgcc tcgatcgtct cgacgggttc
     2641 accctctcgc cggaggcgct ggcgcgcggg ctgcgcgagg tcacctggcc cgcgcgactc
     2701 gaacttctgg cggggggcgc gctggcggcg ctgctgccgg ctggctggga gttgtggctc
     2761 gatggcggcc ataaccctgc cggcggcacc gtgcttgccg cgatggcccg ggaatgggag
     2821 gaccggcccc tgcatctcgt cttcggcatg ctcaagggcc atgacgcggc cggcttcctg
     2881 cggccgctcg cgccctatat cgctggcctc cgcgccgtag ccgttccagg cgaggcgacc
     2941 acgcggagcg ctgaggaggc cgccatcgtc gcccgtgagg ccggtatcgc cgcggtaccc
     3001 tgcggcgacg tcgccgcggc gctcaaggca gcgagctcga gcggatcccg cggccgcgtc
     3061 ttgatctgcg gctcgcttta tctcgccggc catgtgctgg cagagaatgg gcgcggcaca
     3121 atcggcatcg gatagttctg ccgccgccct acaacttggg cggacggtgc ctcgtaccaa
     3181 agagcgactg gccttaaaag acgacagcag gcattggatc accggtgtat gttcatattc
     3241 gtgtaaggtt gccgggtccg ggggcagtgt ttcggggatg ggactgacgg aggagacgcc
     3301 aatgaccgat gtcgctttga gcgcagccgc gcctctgcgg cggacatcgc ccgccgcgag
     3361 cgtcgtcgct ttgaagagcg ccgtccaggc gctccaccac agcgagccgc atttccgcca
     3421 gctcttggag gcactgccgg ccgccgtcta cacgaccgat gcggccgggc ggatcacgtt
     3481 ctacaacgag gcagccgtcg cgctggccgg ccgcaggccg gaattgggta cggatcactg
     3541 gtgcgtcact tggcgcctct accggccgga cggcacgccc atgcgccacg acgagtgtcc
     3601 gatggcgatc gcgctgcgcg agggccgggc cattcgcggc gccgaagccg tcgcggaacg
     3661 cccggacggc agccgcgtgc ctttcatccc ttatccgacc ccgctcttcg atgatgccgg
     3721 gacgctgatc ggcgccgtca acatgctggt cgacatcagc gaccgcaaga aggccgaggt
     3781 ggtgcttcag cagctgaacg acacgctcga gcagcgcatc gaagcgcgca cgcagcaagt
     3841 cagggaaacc ttcacaaaac tctatgaaag cgaacggcgc ttccgcctcc tggtggacgg
     3901 ggtcgtcgac tatgccatct tcatgctcga tcccgacggc ttcgtcacca actggaacag
     3961 cggcgcccaa cgcatcaagg gctatagcgt cagcgagatc gtcggccggc atttctcgtg
     4021 cttctacacc gacgaggacc gggcccgcgg cgtcccggag gcggcgctgc agaccgcctc
     4081 gcgcaccggc cgattcgaag ccgaaggctg gcgccggcgc aaggatggca cgattttctg
     4141 ggcaaacgtg gtcatggacg cgatccacga cgaaacgggc aaactcgcgc ggtttgccaa
     4201 ggtcacgcgc gacttgactg aaaagcgcgc cacggacgag cagcgccgcc aggcgcagaa
     4261 gatggaagcg atcggccagc tcaccggcgg cgtcgctcac gacttcaaca atctgctgac
     4321 ggcaatcatc g
//
LOCUS       DASYNS010000311        14886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2102953, whole genome shotgun sequence.
ACCESSION   DASYNS010000311 DASYNS010000000
VERSION     DASYNS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2102953"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3456
                     /locus_tag="VGP50_09575"
     CDS             <1..3456
                     /locus_tag="VGP50_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEV8027670.1"
                     /translation="DDKYTLASGRVYLSGVQALVRLPMLQRERDKAAGLNTGGFISGY
                     RGSPLGTYDTALWQAKRFLKQHDIHFQPGINEDLAATSLWGTQQVTSFPGATVDGVFG
                     IWYGKGPGVDRSTDPLKHANLAGTSRHGGVIAVAGDDHGCQSSTLAHQSEHIFQAAMI
                     PILNPATVQEYIDLGLYGFALSRFSGCWVGFKAIAETVESSASIDIDPRRVEILLPQD
                     FAMPPDGLNLRSPDPPLDQERRLHGPKMAAIAAFARANRIDRLEINPREARLGIITTG
                     KAYLDVRQALGDLGLDDRAAAALGIRLYKVGLTWPLEPSGARSFAEGLRDVLVVEEKR
                     GFIEDQLMRILYNMDAARRPSVVGKTDESGAPLLPSAGELTPTVVARAIVARLNRQGE
                     ASPPLVQRLARLESFEKMVDAPPPKTQRTPFFCSGCPHNTSTRVPEGSRALAGIGCHA
                     MAMYVPARRTATITHMGGEGANWIGQAPFTEENHIFQNLGDGTYSHSGLLAIRAAAAS
                     GVNITYKILYNDAVAMTGGQPLEGQLSVPQITQQVASEGAKRVVVVTDEPDKYPAGTN
                     FAPGVEIHHRDALDTVQKSLRDTPGLTVLVYDQTCAAEKRRRRKRGLYPDPPRRVFIN
                     ELVCEGCGDCSEQSNCVSVKPVETEFGRKRVIDQSNCNKDYSCLKGFCPSFVTVHGGS
                     LRKLQRPAVADDPLSALPLPALRPLGEPYGILITGIGGTGVITLGALLGMAAHLEGKG
                     CSVLDFTGLSQKNGAVMSHVRLAPSPAALHAVRIAAGGADLLLGCDMVVAASPAALSR
                     VENGVTHTIINSYLAPTAAFVINPDIDFETLTMQRALKAAAGDGHADFIDGTGLATAL
                     MGDSIATNLFMLGYAFQQGLVPLGLAAIERAIELNGVTVESAKRSFGWGRLAAHDRAT
                     VEAAVRPSLREDAPPPESLPALVERRAVFLTEYQDAAYAARYRALVAQVEAAEKTRAR
                     GRSGFAEAVARGLFKLMAYKDEYEVARLYTSGPFLERLGQQFDGDFTLEFHLAPPLVA
                     RPDPYSSTPKKIGFGPWMFRAFTLLARLRRLRGTPFDIFGRTAERRLERQLIVDYEAV
                     IRDIAASLGPDNHALAVEIAEIPQQIRGFGHVKLRNLNAARERETLLFAAFRNPASAA
                     TAAAE"
     gene            3602..4126
                     /locus_tag="VGP50_09580"
     CDS             3602..4126
                     /locus_tag="VGP50_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027671.1"
                     /translation="MPAPFKPVVLLFPILALAACTRSYDRPVVATQPPPPPMLNGALG
                     APIAPDVPLSGSTTPPGPIAIPGAPVRMSAPEITAAFVNNTAEGVTTNGMPYAMYFTA
                     DGSERFKEGNYRDTGTWRVLPDGRFCSSLSEISNGNQECYYMYRTGNAISFERPDGVT
                     VGNVTVVAGDPGAF"
     gene            4447..5814
                     /locus_tag="VGP50_09585"
     CDS             4447..5814
                     /locus_tag="VGP50_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV8027672.1"
                     /translation="MRLLKIAVSAALIGVVPFAAAVTATPDRNDVSVGLFTEVFEQVR
                     SNYVEPVSNKQLVEGAIKGMLGALDPHSSYMDANEYREMMVQTRGEFGGLGMQVTMEN
                     GIVKIISPIDDTPAAHAGLKPGDLILAIDDNPVTDMTLSDAVERLRGPVGTQVKLTMR
                     REGTDAFDVKLTRAEIKVEPVKSHMEGSDIGYVRITNFSERTSTALEAAISSLKSQAG
                     DKLSGVVLDLRNNPGGLLDQAVSVSNAFLDQGEIVSIRGRRAQDNRRFEAEPNHDLLH
                     GLPVVVLINGGSASASEIVAGALHDHKRATLIGTRSFGKGSVQTVLPVKESGGAIRLT
                     TALYFTPSGRSIQATGIEPDVVVEAAKVEKVAQAARPREADLRGALKNTDAEAKAPSG
                     KPASAVEKTTTAPAPNVPPGTTPVARPDALLDANLLGTDADYQLVRAVDLLRGVTLFR
                     HLAAQ"
     gene            complement(6010..6516)
                     /locus_tag="VGP50_09590"
     CDS             complement(6010..6516)
                     /locus_tag="VGP50_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027673.1"
                     /translation="MKSRDKATGKSGRKAGASSLAASAAAPKRIAAEKRSAPSRKSVP
                     DKKGAAKKAAPTPKKAPAAKKPALAPKATPVAAKPAAGAPAPAQAGAKAPPSGTGPSA
                     EERRRAATRKFFARIRSLEAIIGADLRKRGSVVLMQAVDQWYSETINANATEVSITNA
                     AKAVIAQL"
     gene            6941..7939
                     /locus_tag="VGP50_09595"
     CDS             6941..7939
                     /locus_tag="VGP50_09595"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV8027674.1"
                     /translation="MDRVTSMTAFATVVVAGSFAGAAQRLKMSPAMVTNHVRSLEERL
                     GARLLNRTTRKLSLTEAGRSYYEQCALILAQIEAADTSVSALTSTPRGTLRVNAAPIL
                     TLNTAPLIAAYSAAYPEVTLEFTTTDRMVDLVEEGIDMAIRFNQAPDSSLIVRRLGSF
                     RIILCASPTYLAEHGTPREPADLSRHQCVAYMYQGFERLTREWKVTGPKDETLVPITS
                     RIQTNSVETMVAAATEGRGIAMGLSCAVEAAIRAGKLVRVLPEHHLGEYPIIALYPHR
                     QHISAKVRSFLDFAARHFAEMPDCTDAVAAAERAVARFAPAPANAKRSSAAATAVA"
     gene            complement(8190..9128)
                     /locus_tag="VGP50_09600"
     CDS             complement(8190..9128)
                     /locus_tag="VGP50_09600"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV8027675.1"
                     /translation="MLVGGCATEQGAPPAATRAVNTFSLVQGQTEVGSVGTYVTREAD
                     TLLDVARAYDLGYTQLMAANPGIDPWLPGAGRRVTLPSRYLLPEGPRRGIVVNLVQQR
                     LFYFSPDGRTVETYPVGVAVQGRSTPAGTTRVIAKQAHPIWRPPPSIREEQPDLPAMV
                     GPGPDNPLGDYAMRLGWGDYLIHGTNKPDGVGRNSSHGCLRLYPEDIARLFSEVPVGT
                     PVRVVSDEVQAAAIDGELYLAVYPSKDQAEELDTNRKMTPAIPPELKKRLAAAAGPGR
                     ARRIDWEKVAQAARERTGIPVRVSAPTSASVSALMP"
     gene            complement(9432..10715)
                     /gene="urtA"
                     /locus_tag="VGP50_09605"
     CDS             complement(9432..10715)
                     /gene="urtA"
                     /locus_tag="VGP50_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003624681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEV8027676.1"
                     /translation="MRPKLPSLARLAAAAFLLPAIATARAEDTIKVGILHSLSGTMAI
                     SETILKDLMLMQIDELNAHGGLLGKKIEPVVVDPASDWPLFAEKARELLSKDKVAAVF
                     GCWTSVSRKSVLPVFEELNGLLFYPLEYEGEEASYNVFYGGSVPSNKAVPAIEYLMSN
                     EGGAVKRWVLEGTDYVYPRTSNKIIRAFLKSKEVSDDDIRENYTPFGFSDWQTEVAEI
                     KRFAAAGKKTAVISTINGDANVPFYKELANEDVKATDVPVMGFSVGEEELAGIDTKNL
                     IGHLAGWAYFQSVKSAENKTFIDKWRAYTKDPKRVTNDPMEAEYILFHMWAHAVQQAG
                     TTDVDAVRQAMIGQKIDAPSGFVEVMNNNHHLSKPVMIGEIQANGQFAIVFQSKTVLQ
                     PQPWSKYLEEDKGKVADWSYPWRCGNCTAAKYSDF"
     gene            11057..13681
                     /locus_tag="VGP50_09610"
     CDS             11057..13681
                     /locus_tag="VGP50_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV8027677.1"
                     /translation="MRTPIPLLLDRSRSETLIAQLVDQLREAICRGRIAPGERLPSSR
                     SLADQLAIARNTAMRAYEALVIEGYAEARPASGIFAAASLPAPPAPAAPSAPSTAPAD
                     GMHAPLPLPAVPVPGGATPPRGGISFDFSPSAPGAAVFPLKTWRRLLQGALTRGGASG
                     LSQVGDPGGLAALRVALAQHLAAARGIAADPAQILVLSGVREGVALAARLLLASGRRV
                     VLENPVYGAAAAAFAMSGAELLRVSVDQEGLRSEALPELPATLLYVTPSHQYPTGHTL
                     SLARRRAVADWARRQGCYILEDDCGGDFRFEGAPLPAIAALAPDCTIHVGSFARALGA
                     GLRLGYMVAPPPLVEAMRAAKLLLTGSPPWLEQTALADMIRSGSYSAHLARVRAHYRA
                     SRDCLLASLRRHFGEVTVSGESAGQHLLWQLPAGVPDAGTLEALARRARVGVYSLAAA
                     GAGEHPPSLLTRRGLVLGYAALSQRQIEQGIARLSDAVDDTLDRRHEFVDELLLSEPP
                     PAQVARPAPRFRQRPALRPAGAARAFSPRSARREGRAVMPMLRGIYRYPVKGLSPQPL
                     PGIELAAGKPFPHDRTFAFARPGSPIDPDAPRWGKKGLFVMLMLDEALARVQTHLDVE
                     TLAFTVSQDNRQLLRADLGDPASVAELEAFFHRLVPTLRAPPRLVRAPDGHFMDKPDN
                     VLSLINLATLRSLEERWGYAIDPLRFRANFYIDGARPWEEFDWLGGDIVIGDAVFRVD
                     RRNGRCGATNVNPATGQRDLDLPGSLRATFGHKDLGIYLVVRDGGKVVVGDAVTVPRH
                     VTMPADAAPASALAADQRRFICRGCYFVYEETMGAPQSGVAPGTPFAELAAAWRCPDC
                     GTDKSTFRPYVELTAPRV"
     gene            complement(13821..>14886)
                     /locus_tag="VGP50_09615"
     CDS             complement(13821..>14886)
                     /locus_tag="VGP50_09615"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027678.1"
                     /translation="YAVIGKADAAYFRMINEQGGVNGRKIVLLSEDDGYNPAKAVEAT
                     RKLVESDHVAFIFDSLGTPSNTAIQKYLNDRKIPQILLATGADKWGNYKDFPWTMGYQ
                     PSYRTEAQIYAKYILKIRPNAKVAVLYQNDDFGKDYIAGLKDVLGSRYEQMVVKEASY
                     AVTDTTLDTQVITLQASGADTLVTAATPKFAAQVIRKVYSLGWKPTHFLTNVSASIST
                     VIEPAGLEKSIGIISASFQKDPIDPSFKDDADMNQWRDFMRHYMPEADPNDANYVTGY
                     SVSRILVQVLKQCGNDLSRENIIRQAANLKDLSVPTLLPGIKVNTGPMNYHPLRQLQL
                     IRWDGRIWVRFGDVISGAQS"
BASE COUNT         2353 a         5183 c         4847 g         2503 t
ORIGIN      
        1 gacgacaaat acacgctggc gtcgggccgc gtctatctct cgggcgtgca ggcgctggtg
       61 cggctgccga tgctgcagcg cgagcgcgac aaggcggcgg gcctcaacac cggcggcttc
      121 atctcgggtt atcgcggctc gcccttgggc acttacgata ccgctctctg gcaggcgaag
      181 cgcttcctga agcagcacga catccatttc cagccgggca tcaacgaaga cctcgcggcc
      241 acctcgctct ggggcaccca gcaggtcacg tcgtttccgg gcgccacggt cgacggcgtc
      301 ttcggcatct ggtacggcaa ggggccgggc gtcgaccgtt cgaccgatcc gctgaagcac
      361 gcgaatctcg ccggcacgtc gcgccatggc ggcgtcatcg ccgtcgccgg cgacgaccat
      421 ggctgccaat cctcgacgct ggcgcatcag agcgagcaca tcttccaggc ggcgatgatc
      481 ccgatcctga acccggcgac ggttcaggaa tacatcgatc tcgggctcta cggctttgcg
      541 ctgtcgcgct tctcgggctg ctgggtcggc ttcaaggcca tcgccgagac ggtcgagagc
      601 agcgcctcga tcgacatcga tcccaggcgc gtcgaaatcc tgctgccgca ggatttcgcg
      661 atgccgcccg acgggctcaa tctgcgctcg ccggacccgc cgctcgacca ggagcgccgg
      721 ctccatgggc cgaagatggc ggcgattgcg gccttcgccc gcgccaaccg catcgaccgc
      781 ctggagatca atccgcgcga ggcgcgcctc ggcatcatca ccaccggcaa ggcctatctc
      841 gacgtgcgcc aggcgctggg cgatctcggg ctcgacgacc gcgccgcggc agcgctgggc
      901 atccgccttt acaaggtcgg cctcacctgg ccgctcgagc cgtcgggcgc acggtccttc
      961 gccgagggct tgcgcgacgt tctggtggtc gaggagaagc gcggcttcat cgaggatcag
     1021 ctgatgcgga tcctctacaa catggacgcg gcgcgccgcc cgtcggtcgt cggcaagacc
     1081 gatgagagcg gggcgcccct cctgccgagc gccggcgagc tcacgccgac cgtcgtcgcc
     1141 cgcgccatcg tcgcccgcct caaccggcag ggcgaggcga gcccgccgct ggtgcagcgc
     1201 ctggcacgcc tcgaatcctt cgagaagatg gtcgacgcgc cgccgccgaa gacgcagcgc
     1261 acacctttct tctgctcggg ctgtccccac aacacctcga cgcgggtgcc tgaaggcagc
     1321 cgcgcgctcg cgggcatcgg ctgtcacgcc atggcgatgt atgtgccggc gcggcgcacg
     1381 gcgaccatca cgcatatggg cggcgaaggc gccaactgga tcggccaggc gccgttcacg
     1441 gaagagaatc atatcttcca gaatctgggc gacggcacct actcgcatag cgggcttctc
     1501 gcgatccgcg cggcggccgc gtccggcgtc aacatcactt acaagatcct ctacaacgac
     1561 gccgtcgcca tgaccggcgg ccagccgctc gaaggccagc tcagcgtgcc gcagatcacg
     1621 cagcaggtgg cctccgaagg ggcaaagcgc gtcgtcgtgg tgaccgacga gcccgacaag
     1681 tatcccgccg gcacgaactt cgcgccgggc gtcgagatcc accatcgcga cgccctcgat
     1741 accgtgcaga agagcctgcg cgacacgccc ggtctcaccg tgctcgtcta cgaccagacc
     1801 tgcgccgcgg agaagcgccg gcggcgcaag cgcggcctct accccgatcc gccgcgccgc
     1861 gtcttcatca acgagctcgt ctgcgaaggc tgcggcgatt gctccgagca atcgaactgc
     1921 gtgtcggtga aaccggtcga gaccgagttc ggcaggaagc gcgtcatcga ccagtccaac
     1981 tgcaacaagg attactcctg cctcaaaggc ttttgcccga gcttcgtcac cgtgcatggc
     2041 ggcagcctgc gcaagctgca gcgcccggcg gttgccgatg accccctgag cgcgctgccg
     2101 ctgccggcgc tgcgcccctt gggcgagcct tatggcattc tcatcaccgg catcggcggc
     2161 accggcgtca tcaccctggg cgccctcctc ggcatggccg cgcatctcga gggcaagggc
     2221 tgcagcgtcc tcgacttcac cggcctttcg cagaagaacg gcgcggtgat gagccatgtc
     2281 cggctcgcgc cctcgcccgc cgcactccac gcggtgcgca tcgccgcggg cggcgccgat
     2341 ttgctcctcg gctgcgacat ggtggtggcg gcgagcccgg cggcgctgtc gcgcgtcgag
     2401 aacggcgtca cgcacacgat catcaacagc tatctggcac cgaccgctgc gttcgtcatc
     2461 aatcccgaca tcgatttcga gacgctgacg atgcagcggg cgctgaaagc ggcggcgggc
     2521 gacggccacg ccgacttcat cgacggcacc gggcttgcca ccgcgctcat gggcgattcg
     2581 atcgccacca acctcttcat gctgggctac gccttccagc aaggcctggt gccgctcggc
     2641 ctggccgcca tcgagcgcgc aatcgagctc aacggcgtca ccgtcgagag cgcgaagcgc
     2701 agcttcggct ggggccgcct cgccgctcac gaccgcgcga cggtcgaggc ggcggtgcgg
     2761 cccagcttgc gcgaggacgc gccgccgccc gagagcctcc ccgccctcgt cgagcgccgc
     2821 gccgtcttcc tcaccgagta ccaggacgcg gcctacgcgg cgcgctatcg cgcgctcgtg
     2881 gcgcaggtcg aagcggcgga gaagacgcgg gcgcgcggcc gcagcggttt cgccgaagcg
     2941 gtcgcgcgcg gcctcttcaa gctcatggcc tacaaggacg agtacgaggt ggcgcggctc
     3001 tatacgagcg gcccgttcct cgagcgcctc ggccagcaat tcgacggcga tttcacgctc
     3061 gagttccatc tggcgccgcc gctcgtcgcg cgccccgatc cttatagcag cacgccgaag
     3121 aagatcggct tcgggccctg gatgttccgc gccttcacgc tgctggcgcg gctgcgccgg
     3181 ctgcgcggca cgcccttcga catcttcggc cgcaccgccg agcggcgctt ggagaggcag
     3241 ctcattgtcg attacgaggc cgtgatccgc gacatcgcgg cgtcgctcgg ccccgacaac
     3301 cacgcgctgg ccgtcgagat cgccgagatc ccgcagcaga tccgcggttt cggccatgtg
     3361 aagctacgca acctcaatgc ggcgcgcgag cgcgagaccc tcctcttcgc cgctttccgc
     3421 aacccggcgt cagccgcgac cgccgccgcg gaataataac tggctcggtg cgggctctcg
     3481 ccgttgcaag agcaacggct gccgcccgcc gcggacgaag gtgctccgca ccttcgctcg
     3541 gcggaactgc acgccgcgcc gtcgtgttgg cagaacagac agtccgccat gaggtgccaa
     3601 catgcctgcc ccgttcaagc ccgtggtgct gctgttcccg atcctcgcgc ttgcggcctg
     3661 cacgcgctcc tatgaccggc cggtcgtcgc cacacagccg ccgcccccgc cgatgctgaa
     3721 cggcgcgctg ggcgctccga tcgcccccga tgtgccgctc tccggcagca cgacgccgcc
     3781 cggaccgatc gcgattccgg gagcgcccgt ccgcatgtcg gcccccgaga tcacggccgc
     3841 gttcgtcaac aacacggccg aaggggtgac caccaacggc atgccgtatg ccatgtactt
     3901 caccgccgac ggcagcgagc gcttcaagga aggcaattat cgcgacaccg gcacttggcg
     3961 cgtgctgccg gacggccgtt tctgctcctc gctgagcgag atcagcaacg gcaaccagga
     4021 atgctattac atgtaccgca ccggcaatgc catcagcttc gagcgtccgg acggcgtcac
     4081 cgtcggcaac gtcaccgtgg tggccggcga tcccggggcg ttttagcccg gatttctccg
     4141 agagggttcg caaggttctc gagaaaaggc cccgctttgc ggggcctttt cgctggccct
     4201 tgcgcgggat aaactcggct cccggaaatt gggccgagga tgcgccgatg gtaggcgtcg
     4261 cgacgccggg aaggatggcg gcccttgcaa attcgccatt ttgcaagaga tcggcgctta
     4321 tgattttaat cctgtcggct gaacggcctc ttggcctccg ttgttaagat ttaccttgat
     4381 catcatatga gtggacagcc gacggccgcc gtaccaatct gtgccaccca ttgaagagcc
     4441 cccgccatgc gcctccttaa aatcgccgtc tcggccgccc tgatcggcgt cgtgcctttt
     4501 gccgccgcgg tgacggccac gcccgaccgc aacgacgtca gcgtcggctt gttcaccgaa
     4561 gtgttcgagc aggtgcgcag caactatgtc gagccggtct ccaacaagca gctcgtcgaa
     4621 ggcgcgatca agggcatgct gggcgcgctc gacccgcatt cgagctacat ggacgccaac
     4681 gaatatcgcg agatgatggt gcagacccgc ggcgagttcg gcgggctcgg catgcaggtc
     4741 acgatggaga acggtatcgt caagatcatc tcgccgatcg acgacacgcc ggccgcgcat
     4801 gcgggcctga agcccggcga cctgatcctg gcgatcgacg acaatccggt gaccgacatg
     4861 accttgagcg acgccgtcga gcggctgcgc ggtcccgtcg gcacgcaggt caagctgacg
     4921 atgcgccgcg agggaaccga cgctttcgac gttaagctca cccgcgccga gatcaaggtc
     4981 gagccggtca agtcgcatat ggaaggcagc gatatcggct acgtccgcat caccaatttc
     5041 agcgagcgca ccagcaccgc gctcgaggcg gcgatcagtt cgctcaagag ccaggcgggc
     5101 gacaagctgt ccggcgtcgt cctcgacctg cgcaacaacc cgggcgggct cctcgaccag
     5161 gcggtcagcg tctccaacgc cttcctcgac cagggcgaga tcgtgtcgat ccgcggccgc
     5221 cgcgcccagg acaatcgccg tttcgaggcc gagccgaacc acgacctgct gcacggcctg
     5281 ccggtggtgg tgctgatcaa cggcggctcg gcatcggcct ccgagatcgt tgccggagcg
     5341 ttgcatgatc acaaacgcgc gaccctgatc gggacccgct cgttcggtaa gggctcggtg
     5401 cagaccgtgc tgccggtcaa ggagagcggc ggcgccatcc gcctcaccac cgcgctctac
     5461 ttcacgccct ccggccgctc gatccaggcg accggcatcg agcccgacgt cgtcgtcgag
     5521 gcggcgaagg tcgagaaagt ggcgcaggcg gcgcggcccc gcgaggccga tctccggggc
     5581 gcgctcaaga acaccgatgc cgaggcgaaa gcaccgtccg gcaagccggc gagtgccgtc
     5641 gagaagacga ccaccgctcc cgcccccaac gtcccgcccg gcacgacccc ggttgcccgg
     5701 cccgacgccc tgctcgacgc caacctgctc ggcaccgacg ccgactatca gctggtccgc
     5761 gcggtcgacc tgctgcgggg agtgacgctg ttccggcatt tagcggccca gtagtagagg
     5821 gcagagcccg tgattgcccc tctcccgcat cgcgggagag ggagggaccc actgcgaagc
     5881 tttagcgaag cagtgggagg gtgagggggc ggttgcccca ggaacctctg cttgctctgc
     5941 gtggcctctc gccgttgcaa gagcaacggc tggcgcccac ctcaacttta ggcgtgcgct
     6001 agcgcacgcc taaagttgtg caatgaccgc cttcgccgcg ttggtgatgc tgacttcggt
     6061 ggcgttggcg ttgatggtct cgctgtacca ctgatcgacg gcctgcatca gcacgacgct
     6121 gccgcgcttc ctcaggtcgg cgccgatgat cgcctcgagc gagcggatcc gggcaaagaa
     6181 tttgcgcgtc gccgcccgcc gccgctcctc ggccgaggga ccggtgccgg agggtggcgc
     6241 cttggccccg gcttgagccg gcgccggtgc gccggcggcc ggtttggccg cgacgggcgt
     6301 ggccttgggc gcgagggccg gttttttggc cgcaggggcc ttcttgggcg tcggcgcagc
     6361 ctttttcgcc gcgcctttct tgtcgggtac agacttccgc gatggggccg agcgcttctc
     6421 ggcggcaatc ctcttgggcg ccgcggcgct ggcggcgagg gagcttgcgc cggccttcct
     6481 gccgctcttg cccgttgctt tatcccgcga tttcatcagc gtcctccaaa ggcgcctgcg
     6541 aacgcgcaat aaaagtggaa cccgcgcccc cgtctcggca agagaaaagc gcatggggtc
     6601 accgtcgccg agaaactgcg cggcgcagca tagccaaagc cgcccaagcg gggcattcgt
     6661 tgctaaaagg ttctctcctt tgaccacagg agcatccgct cctcagactt tgggcgggtg
     6721 ccgcgactgg gcggcgccgc gactgctctt ggcgcggcga atcggcgact ggccggagcg
     6781 ggcgggcgcg ggctaatcct cccaccttcg cggacgacct gcctccggcg caacccagat
     6841 cttctttgat ctctggaacc ggctgcgcta atggctatct aaaggcaaaa acgggaatgt
     6901 ccagagacgg ccgtccaaca gcgaacgaag cgagaccttg atggaccgag tcaccagcat
     6961 gaccgccttc gccaccgtcg tcgtcgccgg cagcttcgcc ggtgcggccc agcgcctgaa
     7021 gatgtcgccg gccatggtga cgaaccacgt gcgctcgctc gaggagcggc tgggcgcccg
     7081 gctcttgaac cggacgacgc gcaagctcag cctcaccgag gccgggcggt cctattacga
     7141 gcaatgcgcg ctgatcctgg cgcagatcga agccgccgac accagtgtca gcgccctcac
     7201 ctcgacgccc cgcggcacct tgcgcgtcaa tgccgcgccc atcctcaccc tcaacaccgc
     7261 gccgctgatc gccgcctata gcgccgccta tcccgaggtc acgctcgaat tcaccaccac
     7321 cgaccgcatg gtcgacctgg tcgaggaagg catcgacatg gcgatccgct tcaaccaagc
     7381 gccggattcg agcctgatcg tccggcgtct cggatccttc cggatcatcc tctgcgcgtc
     7441 ccctacctat ctcgccgagc atggcacgcc gcgcgagccc gccgacctct cgcgccatca
     7501 atgcgtggcc tacatgtatc agggcttcga gaggctgacg cgcgaatgga aggtcaccgg
     7561 acccaaggac gagaccctgg tgccgatcac gagccgcatc cagaccaaca gcgtcgagac
     7621 catggtcgcc gccgccaccg agggccgggg catcgccatg ggattgagct gcgccgtcga
     7681 ggcggcgatc cgggccggca agctggtccg ggttctcccc gagcatcact tgggcgagta
     7741 cccgatcatc gcgctttacc cgcaccgcca gcacatctcg gccaaggtcc ggagcttcct
     7801 cgacttcgcc gcccggcact tcgccgagat gcccgactgc accgacgcgg tcgccgccgc
     7861 ggagcgcgcc gtcgcccgct tcgccccggc cccggcgaac gcgaagcgtt cgtccgcggc
     7921 ggcgacagcg gttgcgtagg cgcggcgaac gcgcaaagcg cgttcgtccg cggtggcgtc
     7981 agcggttgcg aagcaaccgc gggagccaca cccccgcagg cacggaaaga aacccctcgc
     8041 cccttgtggg agagggaggg gcccgtcgcg aagcgacggg agggtgaggg gtcagcgccc
     8101 cgctgaatct ttgcttgctc tgtgcggctc tcgcggttgc tgacgcaacc gctgccgccg
     8161 ccacggacga aggcgctgcg cgccttcgcc tagggcatca gggcggagac cgaagccgac
     8221 gtgggcgccg agacgcgcac gggaatccct gtccgctcgc gggcggcttg cgcgaccttt
     8281 tcccaatcga tgcgccgagc gcggcctggt ccggccgccg cggcgagccg cttcttgagc
     8341 tccggcggga tggcgggggt catcttccgg ttggtgtcga gttcctccgc ctggtccttg
     8401 ctcgggtaga cggcgagata aagctcgcca tcgatcgcgg ccgcctgcac ctcgtcgctc
     8461 accacgcgaa ccggggtgcc caccggcacc tcgctgaaga gccgcgcgat gtcttcggga
     8521 tagaggcgaa ggcagccatg gcttgagttg cggccgacgc catcgggctt gttcgtgccg
     8581 tggatcagat agtcgcccca tccgagccgc atcgcataat ccccgagcgg gttgtcggga
     8641 cccggaccga ccatggccgg cagatccggc tgctcttcgc ggatcgaggg cggcggacgc
     8701 cagatcggat gcgcttgctt ggcgatcacc cgcgtcgtgc cggccggcgt cgaccgcccc
     8761 tggacggcca ccccgaccgg gtaggtctcg acggtcctgc cgtcgggcga gaagtagaag
     8821 agacgctgct gaaccaggtt gacgacgatg ccacggcgcg gtccttcggg cagcaggtag
     8881 cggctcggca gcgtcacgcg ccggccggcg cccggcagcc acggatcgat gcccgggttc
     8941 gcagccatga gctgcgtgta gcccaaatca taggcgcgcg cgacgtcgag cagcgtgtcc
     9001 gcttcgcgcg tcacataggt gccgaccgag cccacttccg tttgaccctg aacgagggag
     9061 aaggtgttga ccgcccgcgt ggcagccggc ggcgcgcctt gctcggtcgc gcagccgccg
     9121 accagcatcg cggctccgag gccgagaacg agaaggtgtt ggtgccgcat tgcgttgacc
     9181 tagcgccaac aggcgcatcg cgagcgtgtc aatttttgtc gagccgggcc gactgaccgg
     9241 gcacggaagc gtcccaggtc tgcgcccgcg gagaaatcgg gtattcaccc gaggttgctc
     9301 caaacggccc accgctcaag gacgatggcg cgcgcttgag ccgagcggcc gagggcggaa
     9361 tgcccgtgca aaaaaagaag ggctggtttc ccggccctcc gccgcgatgg ttcgcgtcga
     9421 tgccttcgcg ttcagaaatc gctgtacttg gcagcggtac aattgccgca gcgccacgga
     9481 tatgaccagt ccgccacctt gcccttgtcc tcctcgaggt atttgctcca gggttgcggc
     9541 tgcagcaccg tcttgctctg aaagacgatg gcgaactgac cattggcctg aatctcgccg
     9601 atcatcaccg gtttcgagag gtggtggttg ttgttcatga cctcgacgaa gccggacggc
     9661 gcatcgatct tctggccgat catcgcctgg cggacggcgt cgacatcggt cgtgccggcc
     9721 tgttggacgg cgtgagccca catgtggaag agaatgtatt ccgcctccat cggatcgttg
     9781 gtcacccgtt tcggatcctt cgtgtaggcg cgccatttat cgatgaaggt cttgttctcc
     9841 gccgacttga ccgactggaa gtaggcccag ccggcgaggt gcccgatcag attcttcgtg
     9901 tcgatgccgg cgagttcttc ctctccgacg gagaagccca tgaccgggac gtccgtcgcc
     9961 ttcacatcct cgttggcaag ctccttatag aagggaacat tggcgtcgcc gttgatcgtc
    10021 gagatcaccg ccgtcttctt gccagccgcg gcgaatcgct tgatctccgc tacttccgtt
    10081 tgccagtcgg agaagccgaa cggcgtgtag ttctcgcgga tgtcgtcgtc gctgacttcc
    10141 ttcgacttga ggaacgcgcg aatgatcttg ttgctggtgc gagggtagac gtagtccgtg
    10201 ccctcgagca cccagcgctt caccgcgccg ccctcgttgc tcatcagata ttcgatcgcc
    10261 gggaccgcct tgttgctcgg caccgagccg ccgtagaaga cgttgtagga cgcttcctcg
    10321 ccctcgtatt cgagcgggta gaagaggagg ccgttcaact cctcgaagac cggcagcacc
    10381 gacttgcgcg acacggaggt ccagcagccg aagacagccg cgaccttgtc tttcgacaac
    10441 agctcgcgcg ccttctcggc gaagagcggc cagtcggagg ccgggtcgac gaccacaggc
    10501 tcgatcttct tgcccaagag gccgccatgg gcgttgagtt cgtcgatctg catcagcatg
    10561 agatccttca agatcgtctc gctgatcgcc atcgtgcccg agagcgaatg caggatgccg
    10621 accttgatcg tgtcctccgc cctcgcggtc gcgatcgctg gcagcaggaa ggcggcggcc
    10681 gcaaggcgcg ccagggaagg aagcttcggt cgcataaggc acactccatg tcgcgtattg
    10741 caatgcggca gatggtgcac gcatacccgc ctgcgccgga gagccattcc gcgaaaaccc
    10801 ggcggcaccg ctgctgccgc gtcggccggg gtcgccttgc gcggaaaatg agcagagaaa
    10861 tctgcgcaca ggcgaatctt gctcaaggtc tgcacaagaa ccgctggaag aactgggctg
    10921 gagtccagtt ggcatcgcct gtgacggagc cagttttgcg cttttcgcca agctggctcc
    10981 gtcgtttgtg cttctctgcc gcgctgcagc atgcctatca ttcgcaccga cgggcattgc
    11041 cggcacgaag atcgcgatgc ggacaccgat ccccttgctg ctcgaccggt cgcgatcgga
    11101 aaccctgatc gcgcaactcg tcgatcagct gcgcgaggcg atctgccgcg gccggatcgc
    11161 tcccggcgag cggcttccct cgtcgcgctc cctcgccgat cagctggcca tcgcccgcaa
    11221 cacggccatg cgggcctatg aggctctcgt gatcgagggc tatgccgagg cgcgtccggc
    11281 ctcgggcatc ttcgcggcgg caagcctgcc ggcgcccccg gctcccgccg caccgagcgc
    11341 gccctcgaca gctccggcag acggaatgca tgcgcccctg ccgcttcccg cggtgcccgt
    11401 tccaggcggc gccaccccgc cgcgcggcgg catctcgttc gatttctcgc cgtcggcccc
    11461 gggcgccgcc gtgtttccgc tcaagacctg gcgccgcctg ctgcagggcg cgctgaccag
    11521 gggcggcgcc agcggcctct cccaggtcgg cgatcccggc ggcctcgcgg cgttgcgcgt
    11581 ggcgctggcc cagcatctgg cggcggcgcg cggcatcgcc gccgaccccg cgcagatcct
    11641 ggtgctgagc ggcgtccgcg aaggcgtggc gctggccgcc cgtctcctgc tcgcatccgg
    11701 ccgacgggtg gtcctcgaaa atcccgtcta tggcgccgcg gccgccgcct tcgccatgtc
    11761 cggtgctgag ctgctgcgcg tcagcgtgga ccaagagggc ctgcgcagcg aagcgctgcc
    11821 ggagctcccg gcgacgctgc tctatgtcac gccctcgcac caatatccga ccgggcacac
    11881 gctgtcgctg gcgcggcgcc gtgccgtcgc ggactgggcg cggcggcagg gctgctacat
    11941 tctggaggac gattgcggcg gcgatttccg cttcgaggga gcgccgctgc cggccatcgc
    12001 cgcgctcgcc cccgactgca cgatccacgt cggcagtttc gccagggcgc tcggcgccgg
    12061 cttgcggctc ggctacatgg tcgcgccgcc gccgctcgtc gaggcgatgc gcgccgccaa
    12121 gctcctgctc accgggagcc cgccctggct cgagcagacg gcgctcgccg acatgatccg
    12181 cagcggcagc tactctgcgc atctcgcccg cgtgcgcgcc cattatcgcg cgagccgcga
    12241 ctgcctgctg gcgtcgctcc ggcgccattt cggcgaggtc actgtcagcg gcgaatccgc
    12301 cggccagcac ctgctctggc agctgccggc cggcgtcccg gatgccggga ctttggaagc
    12361 gctcgcccgc cgcgcccgcg tcggggtcta ttccctcgcc gcggcaggcg ccggcgagca
    12421 tccgccgtcg ctgctgacgc gccgcggcct cgtcctcggc tacgccgcgc tgtcgcagcg
    12481 ccagatcgag caggggatcg cccgcctctc cgacgcggtc gacgacacgc tcgatcgccg
    12541 ccacgaattc gtcgacgagc tgctgctgtc ggagccaccg ccggcgcagg tggcgcgtcc
    12601 ggctccgcgc tttcgtcaac ggccggctct acggccggcg ggggcggctc gggcattctc
    12661 gccacgcagc gcacggcgag agggaagagc cgtaatgccc atgcttcgcg gaatctatcg
    12721 ctatccggtc aagggcttga gcccgcagcc gctgcccggc atcgagctcg cggccgggaa
    12781 gcccttcccg cacgaccgta ccttcgcctt tgcgcggccg ggcagcccga tcgatccgga
    12841 cgcaccgcgc tggggcaaga agggcctctt cgtgatgctg atgctcgacg aggcgctggc
    12901 gcgggtgcag acgcatctcg acgtcgagac actcgccttc acggtcagtc aggataaccg
    12961 acagctgctc cgggccgatc tcggtgaccc cgcatcggtg gcggagctcg aggcgttctt
    13021 tcaccgcctg gtgccgacct tgcgggcacc gccgcggctg gtgcgggcgc cggacggcca
    13081 tttcatggac aagcccgaca acgtgctctc gctcatcaat ctcgcgaccc tgcgcagtct
    13141 cgaggagcgc tggggctatg cgatcgatcc gttgcgattt cgcgcgaact tctacatcga
    13201 cggcgcgcgt ccctgggaag agttcgactg gttgggcggc gacatcgtca tcggcgatgc
    13261 cgtcttccgc gtcgaccgcc gcaacggccg ctgcggcgcg accaatgtca atccggcgac
    13321 cggtcagcgc gatctcgatc tgcccggctc gctgcgcgcg accttcggcc acaaggatct
    13381 cggaatctat ctggtggtgc gggacggcgg caaagtggtg gtcggcgacg ccgtgaccgt
    13441 tccgcgccac gtcacgatgc cagccgatgc ggcacccgca tcggcgctcg cggccgatca
    13501 gcgccgcttc atttgccgcg gctgctattt cgtctacgag gagacgatgg gcgcacccca
    13561 gtccggcgtc gcgcccggca cgcccttcgc cgaactcgca gcggcctggc gctgtcccga
    13621 ttgcggcacc gacaagagca cgttccgccc ctatgtggag ctgacggcgc cccgcgtctg
    13681 acgcgggtcc cgccacgtca cggccgctaa gaacgagtag gcgcagcgcg gcgaacagct
    13741 ccgggtcggc ggcgcggaaa agggggcgcc gcacggcgcc cccttcccga atttaacctc
    13801 aatacgcctg gtagcgagga ctaggattgt gcgccgctga tgacgtcgcc gaagcgcacc
    13861 cagatgcggc catcccagcg gatcagctgc agctgccgca acggatggta gttcatcggg
    13921 ccggtgttga ccttgatgcc tgggagcagg gtgggcacgc tcagatcctt cagatttgct
    13981 gcctgcctga tgatgttctc gcggctgaga tcgttgccgc actgcttcag cacctgcacc
    14041 aggatccggc tgacactgta gccggtgacg taattcgcat cgttcggatc ggcctcgggc
    14101 atgtagtgcc gcatgaagtc gcgccattga ttcatgtcgg catcgtcctt gaaggatgga
    14161 tcgatgggat ccttctggaa ggacgcgctg atgatgccga tcgatttctc gagcccggcc
    14221 ggttcgatca cggtggagat cgacgccgag acgttggtca ggaaatgcgt cggcttccag
    14281 cccagactgt agaccttgcg gatgacctga gctgcgaatt tgggcgtcgc ggccgtgacg
    14341 agcgtgtcgg cacccgaggc ctgcagcgtg atgacttgcg tgtccagcgt cgtgtcggtg
    14401 accgcgtagg acgcttcctt cacgaccatt tgctcatacc ggctgccgag cacgtccttg
    14461 aggccggcga tgtagtcctt accgaaatcg tcattttggt agaggaccgc gaccttcgca
    14521 ttcggccgga tcttgaggat gtatttcgca tagatctgag cttcggtccg ataactgggc
    14581 tgatagccca tcgtccaagg aaaatccttg taattgcccc atttgtcggc gcccgtggcg
    14641 aggaggatct gcgggatctt gcgatcgttg agatacttct ggatcgccgt gttcgatggc
    14701 gtgccgaggc tgtcgaaaat gaaggcgacg tgatcgctct ccacgagctt ccgggtcgcc
    14761 tcgacggcct tggcgggatt gtagccgtcg tcttcgctca agagcacgat cttgcggccg
    14821 ttgacgccgc cctgctcgtt gatcatcctg aaataggcgg catcggcctt gccgatgacc
    14881 gcataa
//
LOCUS       DASYNS010000312         5594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3975541, whole genome shotgun sequence.
ACCESSION   DASYNS010000312 DASYNS010000000
VERSION     DASYNS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3975541"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VGP50_09620"
     CDS             <1..1051
                     /locus_tag="VGP50_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8027679.1"
                     /translation="LLAFGAIAVVLWIGGYDVIAGRMSGGELSAFMFYAVLTAFAVGT
                     ISEVFGDLQRAAGATERLLELLATPSEITAPAHPAALPLPAQGRVAFEGVTFFYPSRP
                     ERPALADVSLAVSPGETVALVGPSGAGKTTIFQLLLRFYDPARGIVRVDGTDARSVDP
                     AELRRRIGVVSQDPVIFGGSAWENIRYGRPEASDRDIRAAAEAAAAAEFVTALPQGFD
                     TFLGEKGVRLSGGQRQRIAIARAILRDAPILLLDEATSALDAESERAVQQALARLSAG
                     RTTLVIAHRLATVLKADRILVMDEGRIIAEGTHQQLLREGGLYARLAALQFTDGGAAR
                     AVFDPLPPPRRSSAV"
     gene            1101..2546
                     /locus_tag="VGP50_09625"
     CDS             1101..2546
                     /locus_tag="VGP50_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8027680.1"
                     /translation="MPSATARPFIVPLIVACALFMENLDSTVIATALPAIARSLSEDP
                     LRLNLAITSYLLSLAVFIPLSGWAADRYGARRVFRAAIVIFTLGSACCGAAHTLPELV
                     AARVLQGMGGAMMVPVGRLVMLRTVAKSELVRAMSYLTVPALVGPVLGPPLGGFIVTY
                     SSWRWIFFINLPIGLLGIALATLFIEDVKEGAEWPLDVWGFVLAGVGLVGLMFGFETA
                     GRGVLPDMLVAVLLAIGVLSLSLYVVHARVHAYPIIDLGLLRIPTFAAAIVGGSIFRI
                     GIGALPFLLPLMLQLGFGLSPFASGLLTFASAAGALSMKMTAAPIIRALGFRRVLIGN
                     AAVSALFLASYSLFRPTTGHWLIFVALLAGGFFRSLEFTGINTLAFADVPPALMSRAT
                     SFQSMAQQLSVSVGVGTGALLLHVTLTITGHGALAADDFYPAFLAVAAISTTSILFFL
                     RLTPQAGAEVSGHRRGGDPPPAARQEPAPRS"
     gene            complement(2547..2870)
                     /locus_tag="VGP50_09630"
     CDS             complement(2547..2870)
                     /locus_tag="VGP50_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEV8027681.1"
                     /translation="MAALPARAQDAGETVFKRNCAICHTTEPGKNKIGPSLAGVVGRK
                     AGSIPDYSYSQANKSSGVTWDEATLDTYLTAPTKFVPGTKMVFAGLKNPDDRTAVIAW
                     LKTQK"
     gene            3154..4503
                     /locus_tag="VGP50_09635"
     CDS             3154..4503
                     /locus_tag="VGP50_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027682.1"
                     /translation="MALIAALLLMPAPAKATPGYARQAGLACEACHTVFPELTPFGRQ
                     FKLNAYTFSNVRQLQDINDKRESTLALSELAPLSIQVQASNTTLGHSIPDQGVANDLS
                     QKNDAQFPQALSLFYTGKIADNFGAFLQLTWTPNNNSVGIDNSELRFADHAAIPAMGV
                     PDFIYGITVNNNPTVQDVWNSTPAWGFPFIASNVTVPFAKTLIDGGLAQESAGLGAYI
                     WAWNHFYLEGTAYRSAQTNFTNGKTGGPGPLDSTAANPRISGVAPYWRAAWEQDFDRH
                     SITFGTFGLLADTHPTGIGEHGPTDRFLDLALDTQYQYIGDVNIFSVLATFIHEKRDF
                     NATVAANPSSHLDTARITGSYFYDRKYGGSVEFFSTTGTTDAVLFAPASVTGSNTGKP
                     DTQGATIELDYLPWLNTKIGVQYTAYGKFNGSSTNYDGFGRSAADNNTLFIFVWAAF"
     gene            4529..5134
                     /locus_tag="VGP50_09640"
     CDS             4529..5134
                     /locus_tag="VGP50_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV8027683.1"
                     /translation="MKPSVFCAALAALLLSAVQASAADKSAALDLVAKECSTCHGAKG
                     ISISSTFPNLAGQQAEYLEVQLKAFRDRSRADPHAQAFMWGMAAQLTDPIIKDIAAYF
                     AAQPPAAGSTADTPEVAAGKKIFDDGIAAQDVPQCQACHGEKAQGNGAFPRLAGQHRS
                     YLEKQLEAFAANLRANDMMHENSKNLTALQISQVAAFLGTQ"
     gene            5144..5482
                     /locus_tag="VGP50_09645"
     CDS             5144..5482
                     /locus_tag="VGP50_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027684.1"
                     /translation="MIHPRHLAFFVAALLAPLPAQAAGGCDKWLSETVQATGSYLPTA
                     EAYSRSFVFALRLDCDGTTELVTVQRPTGNLPVCDAGQSVEVVGRLIWNRTLVDGHYE
                     INDPQGVSCR"
BASE COUNT          850 a         1986 c         1763 g          995 t
ORIGIN      
        1 tctcctcgcc ttcggcgcca tcgcggtggt gctctggatc ggcggctatg acgtgatcgc
       61 cggacggatg agcggcggcg agctctcggc cttcatgttc tatgccgtgc tcacggcctt
      121 tgcggtcggc accatctcgg aggtgttcgg cgatctccag cgcgcggcgg gcgcgaccga
      181 gcggcttctc gagctcctgg ccacgccctc cgagatcacg gcgccggcgc atcccgcggc
      241 gctgccgctt ccggctcagg ggcgcgtcgc cttcgagggc gtcaccttct tctatccctc
      301 gcggccggag cggccggcgc ttgccgatgt ctcgctcgcg gtgtcacccg gcgagacggt
      361 tgcgctggtc gggccttcgg gcgccggcaa gacgacgatc ttccagctcc ttctgcgctt
      421 ctacgatccc gcgcgcggta tcgtgcgcgt cgacggcacc gacgcgcgat cggtcgatcc
      481 cgcggagctc cgccgccgca tcggcgtcgt gtcgcaggat ccggtgatct tcggcggctc
      541 ggcctgggag aacatccgct atggccggcc ggaggcgagc gatcgcgaca tccgcgccgc
      601 cgccgaggcc gcggcggccg ccgagttcgt gaccgctctg ccgcaaggct tcgacacctt
      661 cctcggcgag aagggtgtca ggctctccgg cgggcagcgc caacgcatcg cgattgcccg
      721 cgccattctg cgcgatgcgc cgatcctttt gctggacgag gcgacctccg cgcttgatgc
      781 cgagtccgag cgcgcggtgc agcaggcgct ggcgcggctc tcggcgggac gcaccaccct
      841 cgtcatcgcc catcggctcg ccaccgtgct caaggctgac cgcatcctgg tgatggacga
      901 aggccgcatc atcgccgagg ggacgcatca acagctcctg cgcgaaggcg gcctctatgc
      961 gcgcctcgcg gcgctgcaat tcacggatgg gggcgcggcg cgcgccgtgt tcgacccgct
     1021 tcccccgccg cgccgcagct cggcggtata ggcgaagcac ctgcttttcc gcgcgaccgg
     1081 ctctataacc ttcgcgcctc atgccgtccg cgaccgcccg ccccttcatc gtcccgctga
     1141 tcgtcgcctg cgcgctcttc atggagaatc tcgactccac ggtgatcgcg acggcgctgc
     1201 cggcgatcgc ccgctcgctc tccgaggatc cgctgcggct caacctggcg atcacctcct
     1261 atctgctgag cctcgccgtg ttcattccgt tgagcggctg ggcggccgac cgctacggcg
     1321 caaggcgcgt gttccgcgcg gcgatcgtca tcttcaccct gggctccgcc tgctgcggcg
     1381 ccgcgcatac gctgcccgag ctggtggcgg cacgggtcct gcaagggatg ggcggcgcga
     1441 tgatggtgcc ggtcgggcgg ctcgtgatgc tgcgcaccgt ggccaaatcg gagctggtgc
     1501 gcgccatgtc ctatctcacc gtgccggcgc tggtcgggcc ggtgctcggg ccgccgctcg
     1561 gcgggttcat cgtgacctac agctcctggc gctggatctt cttcatcaat ctgccgatcg
     1621 ggctcctcgg gatcgctctg gccacgctct tcatcgagga cgtgaaggag ggggccgagt
     1681 ggccgctcga cgtctggggc ttcgtcctgg ccggtgtcgg gctcgtcggc ctcatgttcg
     1741 gtttcgagac ggcggggcgg ggcgtgctgc cggacatgct ggtggccgtc ctcctcgcca
     1801 tcggcgttct ctctctgtct ctctatgtcg tccacgcccg cgtccatgcc tatcccatca
     1861 tcgatctggg attgctgcgg atcccgacct tcgccgcggc gatcgtcggc ggctcgatct
     1921 tccgcatcgg catcggcgcc ctgcccttcc tgctgccgct gatgctgcag ctcggattcg
     1981 gcctctcgcc cttcgcctcg ggtctcctta ctttcgccag cgccgccggg gcgctcagca
     2041 tgaagatgac ggcagcgccg atcatccgcg ccttgggatt ccgccgggtg ctcatcggca
     2101 acgccgcggt gagcgcgctc ttcctcgcca gctacagcct attcagaccc acgacggggc
     2161 attggctgat cttcgtggcg ctgctcgccg gcggcttctt ccgctcgttg gaattcacgg
     2221 gcatcaacac gctcgccttc gccgatgtgc cgccggcgct gatgagccgc gccacgagct
     2281 ttcagagcat ggcgcagcag ctctcggtca gcgtcggtgt cggcacgggc gcgctcctcc
     2341 tccatgtcac gctgacgatc accgggcacg gcgcgcttgc cgcggacgat ttctatccgg
     2401 cgtttctcgc cgtggcggcg atctcgacga cgtcgatcct gttcttcctg cgtctgacgc
     2461 cgcaggcggg ggcggaggtc agcggccatc gccgcggcgg ggacccgccg cccgcggcca
     2521 ggcaggagcc ggcgccgcgc tcgtgactac ttctgagtct tgagccaggc gatgacggcc
     2581 gtgcggtcgt cggggttctt gaggccggcg aagaccatct tcgtgcccgg gacgaatttc
     2641 gtcggcgccg tcaaataggt gtcgagtgtc gcctcgtccc aggtgacgcc cgagctcttg
     2701 ttcgcctgcg aatacgagta gtcgggaatg ctgccggcct tgcggccgac gacgccggcg
     2761 aggctcgggc cgatcttgtt cttgccgggc tcggtggtgt ggcagatcgc gcaattcctc
     2821 ttgaacacgg tttcgccggc gtcctgcgcg cgggccggca gcgcggccat cagccccgcc
     2881 agggcgagag aaaggacagc aaggattcgc ttcatggggg tccccgatcg aagtggtcgc
     2941 ttgataaact gagcgccggc ctgttgccgc cgcgccacgc cctagcattc attttttgat
     3001 cgcgatgtcc agacgcggcg attgacatgg atcaaagcga ccgagagcag caaggaatat
     3061 cccagccctc agtagcgcgg atcaagcagc ttctttcttg ccgcgtgcgc gacatagggg
     3121 ggcatgatgc gtccgacacc gccaaagatc gcgatggcat tgatcgccgc gctgctgctg
     3181 atgccggcgc cggcgaaggc gaccccgggc tatgcccgcc aggcgggact cgcttgcgag
     3241 gcctgccaca ccgtcttccc cgagctcacg cccttcggcc ggcaattcaa gctcaacgcc
     3301 tacaccttca gcaacgtcag gcagctgcag gacatcaacg acaagcgcga aagcacgctt
     3361 gcgctcagcg agcttgcgcc gctgtcgatc caggtgcagg cgtccaacac gacgcttggc
     3421 cactcaatcc ccgatcaggg cgtcgccaac gacctctcgc agaagaacga tgcccagttc
     3481 ccgcaggcgc tgagcctctt ctacaccggc aagatcgccg acaatttcgg cgcgttcctg
     3541 cagctgacct ggacgcccaa caacaattcg gtcggcatcg acaattccga gctgcgcttc
     3601 gccgatcacg cggccatccc ggccatgggc gtgccggact tcatctacgg catcaccgtc
     3661 aacaacaatc caaccgtgca ggacgtgtgg aacagcacgc cggcctgggg tttccccttc
     3721 atcgcctcga acgtgaccgt gcccttcgcc aagaccctga tcgatggcgg cctcgcccag
     3781 gaatcggccg ggctcggcgc ctacatctgg gcctggaatc atttttatct ggagggcacc
     3841 gcctatcgct cggcacagac gaacttcacc aacggcaaga ccggagggcc gggaccgctc
     3901 gacagcacgg cggccaatcc gcgcatcagc ggcgtcgcgc cttattggcg cgcggcctgg
     3961 gagcaggact tcgatcgcca ttcgatcacc ttcggcacct tcgggctcct cgcggacacg
     4021 catcccacgg gcatcggcga gcacggcccg accgaccggt tcctcgacct cgcgctcgat
     4081 acgcaatacc agtacatcgg cgacgtgaac atcttctccg tgctcgccac ctttatccat
     4141 gagaagcgcg acttcaatgc gacggtcgcg gccaatccga gcagccatct cgacacggcg
     4201 cgtatcaccg gcagctactt ctacgatcgc aagtatggcg ggagcgtcga gtttttctcg
     4261 accaccggca ccaccgacgc cgtgctcttc gcgccggcct ccgtcaccgg gagcaatacg
     4321 ggaaagcccg acacccaagg cgccacgatc gagctcgact atctgccctg gctcaacacc
     4381 aagatcggcg tgcaatacac cgcctatggg aagttcaacg gcagctcgac caactatgac
     4441 ggcttcggcc gcagcgcggc cgacaacaac acgctcttca tcttcgtctg ggccgcgttc
     4501 tagcgccccc cgcggcagga gtgagatcat gaagccctct gtcttttgtg cggcgctggc
     4561 agccctgctg ctgtccgccg tccaagcttc ggccgcggac aagagcgcgg cgctcgatct
     4621 ggtcgccaag gaatgctcga cctgccacgg ggcgaagggg atcagcatct cgtcgacctt
     4681 ccccaacctc gcgggacagc aggcggagta tctcgaggtg cagctcaagg cgttccgcga
     4741 ccgctcgcgg gccgacccgc acgcccaggc cttcatgtgg ggcatggcgg cgcagctgac
     4801 cgatccgatc atcaaggaca tcgccgccta tttcgccgcg caaccgccgg ccgcgggcag
     4861 caccgccgat acccctgagg tcgccgccgg caagaagatc ttcgacgacg gcatagcggc
     4921 ccaggacgtg cctcagtgcc aggcctgcca tggcgagaag gcgcaaggca acggcgcctt
     4981 cccgcggctc gccggccagc accgcagcta tctcgagaag cagctcgagg cgttcgccgc
     5041 caatctgcgc gccaacgaca tgatgcatga gaactcgaag aacctgacgg cgctgcaaat
     5101 cagtcaggtt gccgccttcc tcggcacgca ataacatcca gcgatgatcc accctcgcca
     5161 cctcgcattc ttcgtcgccg cgctgctcgc gccgctgccg gcccaagcgg cgggcggctg
     5221 cgacaaatgg ctcagcgaga cggtccaggc gaccggttcc tatctgccca cggcggaggc
     5281 ctattcgcgg tccttcgtct tcgccctgcg gctcgattgc gacggcacga cggagctggt
     5341 caccgtccag cgcccgaccg gcaacctgcc cgtctgcgac gccggccagt cggtcgaagt
     5401 ggtgggacgg ctcatctgga atcggacgct ggtcgacggc cattacgaga tcaacgaccc
     5461 gcagggcgta agctgccggt aggcgccgcg ctcgcgagag gggccaaatc cgcgccagca
     5521 tgataatttg acgggccgcg tccctatcac gctcgaggcg agcctctttc atgaccgcca
     5581 tcaacgaaga tatt
//
LOCUS       DASYNS010000313         3069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5068271, whole genome shotgun sequence.
ACCESSION   DASYNS010000313 DASYNS010000000
VERSION     DASYNS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5068271"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..1960
                     /locus_tag="VGP50_09650"
     CDS             314..1960
                     /locus_tag="VGP50_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEV8027685.1"
                     /translation="MDGSEVTKGTSAGPRKAFPRQPGQIGIITAVSGFKVSCLIFGPS
                     TPGGTDGALSAGQIGALVKVPTVSSMAYGFIASLSLAEPTFEAQVAIAEIDLLGEHTG
                     KRSDGSSKFVRGISVYPVLGAPVFATTAEDVGVIYAKPNSWVLQIGTLYQDANTPAFL
                     MSQEFLSKHSAIVGTTGSGKSCALTLILRSLLTEHQNGHVVMLDPHGEYATAFNDIAE
                     LITPDTMQLPYWLLSFEEIVEVLCSEDPTARSREAPILKEVIMAAKQDFLGANANASS
                     LTVDTPTPYRITSVLQRISEQMGKLDRPDGSLPYLRLMNTIENLRKDPRYAFMFEGLT
                     VRDNMAEIISRFLRIPVSGKPITILDISAVPSEIVNVVVSVLARLIFDFALHSEREEA
                     IPVLIVCDEAHRYIPRDESSGFGPTRRAISRVAKEGRKYGVSLCLVTQRPSELSESIL
                     SQCSTVFALRMNNERDQRYVRSMLPDDAAGLLNALPTLRQREAIVVGEGVAHPMRIRF
                     KDLEERHRPQGEAANFPEAWQNDLKGREFVGKIIERWRHQ"
     gene            complement(1962..>3069)
                     /locus_tag="VGP50_09655"
     CDS             complement(1962..>3069)
                     /locus_tag="VGP50_09655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8027686.1"
                     /translation="AFLDLYAPQAVLPRLRDELGVSAGDAGLLVSATTFAIALIAPFT
                     GTVADVLGRKRVIAIAMAALVVPTVLVALATSLPMLVLWRFVQGLLLPPVFAVTIAYI
                     AEEFSPREATGVTGLYISASSLGGFLGRFLTGILAEHLGWRDAFLVLAAVTLACAAGV
                     ALLLPPERGFTRADGVLSAARQMLRHLTDRRLVAIYAVGFGVLFTFVATFTYVNFHLA
                     AAPYRLSPSGLGAIFAVYLMGSLVAPLTGRGVARFGRRRLVVGLIVFWGAGLALTLLP
                     SLGAIVAGLALGVACGFVCQAVATGFVALSVPTGRSSAIGLYATCYYLGGSAGGVLPA
                     LAWNAAGWPGCVALLLGVLLLLAVVVELSWRDVA"
BASE COUNT          611 a         1045 c          982 g          431 t
ORIGIN      
        1 gtgccgccgg ccacctgcgc atctccctcg cggccccgga cagccggttg gccgaagccg
       61 gcggtcgaat cctccgattc acccaggcgg tcgcccggga atcggccgcc acctagtgct
      121 ccacgccgtc tttcgcaagg agacagggaa tttttggcga gtgattcgac tagccgtaca
      181 ggcaaggatt tagccggttt gccgggcaaa tccgccgcga tgcgcaattc gatgattcga
      241 gctggaaagg tttgagcaaa gatttcgcca tagcttcgct gagcgtgcca gcacagccaa
      301 ggcgagggaa ccgatggacg gaagcgaagt cactaaagga acaagtgccg gaccgcgcaa
      361 ggctttcccg cgccaaccgg gccagatcgg catcatcacg gccgtgtccg gcttcaaggt
      421 gtcgtgcctg atcttcgggc cgtcgacgcc gggcggcacg gacggagccc tgtcggccgg
      481 acagatcggc gccctcgtga aggtgccgac ggtgagttcg atggcctacg gcttcattgc
      541 cagcctgagc ctcgccgaac ccaccttcga ggcgcaagtc gccatcgccg agatcgacct
      601 gctgggcgag cacaccggca agcgcagcga tggctcgtcg aaattcgtgc gcggtatttc
      661 ggtgtacccc gttctgggcg caccggtctt cgccaccacg gccgaggacg tcggcgtcat
      721 ctatgccaag ccgaactcct gggtgttgca gatcggcacg ctctaccagg acgccaacac
      781 gcccgccttc ctcatgtcgc aggaattcct gtcgaagcac tcggccatcg tcggcacgac
      841 gggctcgggc aaatcctgcg ccttgacgct gatcctgcgc tcgcttctga ccgagcacca
      901 gaacggccac gtcgtcatgc tcgaccccca cggcgaatat gcgaccgcct tcaacgacat
      961 cgccgaactc atcacgcccg acacgatgca gcttccttac tggctcctga gcttcgagga
     1021 gatcgtcgag gttctttgca gcgaggatcc gaccgcccgc agccgcgagg caccgatcct
     1081 caaggaagtc attatggcgg cgaagcagga cttcctcggc gccaacgcca acgcctcctc
     1141 gctcaccgtc gacacgccga cgccctatcg catcaccagc gtgctccagc gcatctccga
     1201 gcagatgggc aagctcgacc ggcccgacgg ctccctgccc tatctgcgcc tcatgaacac
     1261 catcgagaat ctgcgcaagg acccgcgcta cgccttcatg ttcgagggcc tgaccgtgcg
     1321 cgacaacatg gcggagatca tctcgcgctt cctgcgcatc ccggtctcgg gcaaacccat
     1381 caccatcctc gacatctcgg cggtcccgtc cgagatcgtc aacgtcgtgg tgtcggtcct
     1441 cgcccgtctt atcttcgact tcgcgctgca tagcgagcgc gaggaagcga tccctgtgct
     1501 catcgtctgc gacgaggcgc atcgttacat tccgcgcgac gaaagctcgg gcttcgggcc
     1561 gacacggcgg gccatctcgc gcgtcgccaa ggaagggcgc aaatacggcg tctcgctgtg
     1621 cctcgtcaca cagcgcccgt ccgagctctc ggaatcgatc ctgtcacaat gcagtaccgt
     1681 ctttgcgctg cgcatgaaca acgagcgcga tcagcgctac gtgcgcagca tgctgccgga
     1741 cgatgccgcc ggcctcctga atgcgctgcc gacgctgcgc cagcgcgagg cgatcgtcgt
     1801 cggcgaaggc gtggcgcacc ccatgcgcat ccgcttcaag gacctcgagg aaagacatcg
     1861 cccgcaaggc gaggcagcga acttccccga ggcatggcag aacgacctca agggccggga
     1921 gttcgtcggc aagatcatcg agcgctggcg ccaccaataa gctaagccac gtcgcgccag
     1981 gagagctcga cgacgacggc caacagcaag agcacgccca gcagcagggc gacgcagccg
     2041 ggccagccgg cggcgttcca ggcgagcgcc ggcagcaccc cgccggcgct gccgcccagg
     2101 tagtagcagg tcgcataaag gccgatcgcc gatgagcgcc cagtcggcac gctcagcgcg
     2161 acgaagcccg tggcgacggc ttggcagacg aaaccgcaag caacacccaa ggcgagcccc
     2221 gcgacgatgg cgccgagcga gggcaggagc gtgagcgcaa ggccagcgcc ccagaagaca
     2281 atcagaccga cgacgagccg ccgccggccg aaccgggcaa cgccgcgccc agtgagcggc
     2341 gccaccaggg accccatcag atagacggcg aagatcgcgc cgagcccgct cggcgagagg
     2401 cgatagggcg cagcggccag gtgaaaattt acgtaggtga aggtggcgac gaaggtgaag
     2461 agcacgccga agcccacggc atagatcgcc acgagccgcc ggtcggtcag atgacgcagc
     2521 atctgtcgcg ccgcgctcag cacgccgtcc gcacgggtga agcctcgctc gggcgggagc
     2581 agcagagcaa ctccggcggc gcaggcgagc gtcaccgctg cgagcacgag aaaggcatcc
     2641 cgccagccga gatgttcggc gagaatgccc gtgagaaagc ggccgagaaa gccgccaaga
     2701 ctgctggcgg agatatagag accggtgacg ccagtcgcct cgcgcggcga aaattcctcg
     2761 gcgatatagg cgatggtgac ggcgaagacc ggcggcagca gcaatccctg gacgaagcgc
     2821 cacagaacga gcatcggcaa gctcgtcgcc aacgccacca agaccgtcgg caccacgagc
     2881 gccgccatgg cgatggcgat cacgcgcttg cggccgagca cgtcggcgac ggtgccggta
     2941 aagggggcga tgagcgcgat ggcaaaagtg gtggcgctca cgaggagccc ggcgtcgccg
     3001 gcgctgaccc cgagctcgtc gcggagccgc ggcagcaccg cctgcggtgc gtatagatcg
     3061 aggaaggcg
//
LOCUS       DASYNS010000314         5761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1793589, whole genome shotgun sequence.
ACCESSION   DASYNS010000314 DASYNS010000000
VERSION     DASYNS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793589"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1060
                     /locus_tag="VGP50_09660"
     CDS             179..1060
                     /locus_tag="VGP50_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV8027687.1"
                     /translation="MDAADLRIFEAVARLGGMNRAAAELNTVQSNVTARIRLLEKELE
                     APLFRRHSRGVALTDAGRRLLPYATRIEHLLRDARSAVADDGHPKGTLTVGALETTAA
                     LRLSSHLSSYVAQFPKVDLILRTGTTQELIDDVLDHRLEGAFVCGPVDHPDLEQSHAF
                     REELAILTAPSIRTLDALFREAEVRIVVLRAGCSYRRRLEDLLAKRGILSPRRLEFGS
                     LEAIVGCVAAGLGVTLLPKSLVGAVTRDHDLVVHPLPKGEGRVDTIFIHRRDAYMSSA
                     LTAFLRRVCVPATAAAE"
     gene            1170..2255
                     /locus_tag="VGP50_09665"
     CDS             1170..2255
                     /locus_tag="VGP50_09665"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEV8027688.1"
                     /translation="MTTTPTHPALARAGAFCERFGLRLPILLGPLAGASPPSLSIAVA
                     NAGGLGACGALPMEPQEIIDWAAELRAGSNGAFQLNLWVPDPPPRRDRKHEADLRTFL
                     AQWGPEAPADAGNAAPPDFAAQLEALLAAAPPIVSSVMGLYPPEFVSRLKARKITWFA
                     NVSTVAEAKAAEAAGADVIVAQGMEAGGHRYCFDAARAEREMVGLFALIPAVVDAVKV
                     PVVATGGIADGRGVAAALLLGASAAQIGTAFLRCPEAKIHPAWAAALAAALPEDTVVS
                     RAFSGRAGRSIATDYVRAAMGPGAPPPAPYPVQRGLTAALRAAGGKAGDVHRMQVWAG
                     QAAALARAEPAAAVVNRLWHEATALLC"
     gene            2301..2504
                     /locus_tag="VGP50_09670"
     CDS             2301..2504
                     /locus_tag="VGP50_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027689.1"
                     /translation="MAYARAYNQNSESVAFYEFTRTMQSYKSIISENTTLILSTGSDL
                     FKFLKGMAPDGHVVPALHASDNR"
     gene            complement(2524..2700)
                     /locus_tag="VGP50_09675"
     CDS             complement(2524..2700)
                     /locus_tag="VGP50_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027690.1"
                     /translation="MTGDDAGRIVRGSGFGHIGENVRLYRDDSGTPSALRFGANQLLP
                     EHLLAREMTERYEG"
     gene            3109..3453
                     /locus_tag="VGP50_09680"
     CDS             3109..3453
                     /locus_tag="VGP50_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV8027691.1"
                     /translation="MLREIAQVIAAQGEYRIGEWFTEDFRLIEPTKPNWPRGHEGARK
                     LLEAFRTLRPPIEFVALDMVEEGHRVAVRWQLSATHQGERLPLASIAIYRFEKGLIAE
                     DWGIPIRGEWLD"
     gene            3590..4606
                     /locus_tag="VGP50_09685"
     CDS             3590..4606
                     /locus_tag="VGP50_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV8027692.1"
                     /translation="MRGAVEDVARGRLAGFLAAAAGARRVAIEALRLLPGGAVQENWL
                     IEARLAGGPLEGCQRFVLRKAAPTTLTLSHDAATEFAVLRAVQDAGVAVPEPLFLCRD
                     PAMLGKPFLVMRWVPGVADGALLAADAVNEALAERLGRELALLHRIVPPRAGLAGLGA
                     PPVDAAAARLAQYRDMLDASGDAHPVAEWGMRWLLRHAPPPAAPVLCHGDFRTGNYLV
                     DEGRLTAVLDWEFAGWGDPDEDIGWFCLGCWRFGAYERGAGGIAPRAAFLRGYIAASG
                     RRLDPARLRYWEAMAALRWLVIALGQRDRFLRGGERSLDLALTGRRPAECELEILRLT
                     AEPA"
     gene            4606..4968
                     /locus_tag="VGP50_09690"
     CDS             4606..4968
                     /locus_tag="VGP50_09690"
                     /inference="COORDINATES: protein motif:HMM:NF040046.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HEV8027693.1"
                     /translation="MRDRPDGTQLLALARELEEIAPRLPPEERVDPALLARAQAIAER
                     ERQAGDAPLAACREALVALYGPGDFTDLFERLAREIRAGTYDAAGAERDRVCRVLWAV
                     TLQKLRESNPEYLAASAR"
     gene            complement(5138..>5761)
                     /locus_tag="VGP50_09695"
     CDS             complement(5138..>5761)
                     /locus_tag="VGP50_09695"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEV8027694.1"
                     /translation="YGRVREWDVFLARTVGQLRQTMPEHPALGLLVERALAARRKALP
                     QGDALKASIAAVEAAVEEAPWLHRPTDLLASGWERPLAHYAGELLEKRHRKLRRAVKQ
                     VELADQIAFHELRLRVKKLRYPAELLKSLFDERGAAAYLERLVTLQSVLGGLNDALAD
                     QALVAALRLPLETQHLVLGWLGHEMESLRQRFPDAAHAVRGAKVFWS"
BASE COUNT          886 a         1890 c         2017 g          968 t
ORIGIN      
        1 tgctggcgac ggccgttttg gcgctgacgc gctgaagctg tgccgctgcc gcttcgacgc
       61 gcgccggcaa caggccgcgc tcagactttc gccagtatcg cccgcttgag ctcgccggcg
      121 agcggcaacg acgatcgtgc atgcgacata cgatcgtttt ctatgatatc atcaggcgat
      181 ggacgccgct gacctccgca tcttcgaggc cgtcgcccgg ctgggcggaa tgaaccgcgc
      241 ggccgccgag ctgaacaccg tgcaatcgaa cgtcacggcg cgcatccgct tgctcgagaa
      301 ggaactcgaa gcgcctttat tccgcagaca cagcagaggc gtcgccctca cggatgccgg
      361 gcggcggctg ttgccgtatg cgacacgtat cgagcatctc ttgcgcgacg cgcgaagcgc
      421 ggtcgccgac gacggtcacc ctaaggggac gctgacggtg ggtgccctgg aaacgaccgc
      481 agcgcttcgt ctttcgtcgc atctctcgag ctacgtcgca caatttccga aggttgatct
      541 gatcctccga accggaacga cccaggaatt gatcgacgat gtcctcgatc atcgattgga
      601 gggcgccttc gtctgcggac cggtggacca tccggacctc gagcaaagtc atgcattccg
      661 cgaggagctg gcgatcctga ccgcgccgtc gatacgaact ctggatgcgc tgttcagaga
      721 agcggaggta aggatcgtcg tccttcgcgc gggttgctca tatcgccggc gcttggagga
      781 tcttctggcg aagcgcggca ttctcagccc gcgcaggctc gaattcggct ccctggaggc
      841 cattgtcgga tgtgtcgccg ccggcttggg cgtgaccttg ctgcccaaga gcctcgtcgg
      901 cgcggtgaca cgcgatcacg acctcgtcgt acatccgctt ccgaaagggg aggggcgcgt
      961 ggacacgata ttcatccatc ggcgtgatgc ctatatgtcc agcgcgctca cggcctttct
     1021 gcgtcgcgtc tgtgtccccg cgacggcggc agcggaatag cggcgatatc ttcatttgag
     1081 atcgctgcaa tcgctttaaa tcgcttttga tgattctcac ctccgtatag cattccccga
     1141 tccaaccatg ggcgggagga atgccgcaga tgacgacgac accgacgcat cctgctcttg
     1201 cccgcgcggg tgccttttgc gagcggtttg gtctgcgcct gccgatcttg ctggggccgc
     1261 tggccggcgc ctctccgccg tcgctttcca tcgccgtcgc caacgccggc gggcttggcg
     1321 cctgcggtgc gcttcccatg gagccgcagg agatcatcga ctgggcggca gagttgcgcg
     1381 ccggctcgaa cggcgccttt cagctcaatt tgtgggtgcc cgatccgccg ccgcggcgcg
     1441 atcgaaaaca cgaagcagat ctgcggacgt ttctggcgca atggggcccc gaggcgccgg
     1501 ccgatgccgg caacgcagca ccgcccgatt ttgccgcgca gctcgaggcg ctgctggcgg
     1561 cggccccgcc gattgtctct tcggtgatgg gactataccc gcccgaattc gtgtcgcggc
     1621 tcaaggcccg gaagatcacc tggttcgcga atgtttccac cgtggcggag gcgaaggccg
     1681 ccgaggctgc cggcgccgac gtgatcgtcg cgcagggcat ggaagccggc ggccatcgct
     1741 actgtttcga cgccgctcgg gccgagcgcg agatggtcgg gctcttcgcg ctcattcccg
     1801 ccgtcgtcga cgcggtcaag gttccggtcg ttgcgacggg cggtatcgcc gacgggcgcg
     1861 gcgtcgccgc ggcattgctc ctcggcgcga gcgctgccca gatcgggaca gctttcctgc
     1921 gctgccctga ggcgaagatc catccggcct gggccgcggc cctcgccgcc gccctcccgg
     1981 aagataccgt cgtcagccgg gctttcagcg gacgcgcggg gcgcagcatt gcaacggact
     2041 acgttcgagc ggccatgggc cccggcgcgc cgccgccggc gccctatccg gtgcagcggg
     2101 gcttgacggc cgcgctgcgc gccgccggcg gcaaagcagg cgacgtgcac cgaatgcagg
     2161 tttgggccgg gcaggccgcg gctcttgccc gcgcagagcc ggccgcagca gtcgtcaatc
     2221 gtctttggca tgaggcaacg gcgctgctct gttgatccgc cgatccgtcg acattcagat
     2281 tctcgagatc ggaaccgggc ttggcctacg cgcgggccta caatcagaac tcggaatcgg
     2341 tggcgttcta tgaattcacg cggaccatgc agtcctacaa atccatcatc agcgagaaca
     2401 cgacgctcat cctctccacg ggcagcgacc tcttcaagtt cctgaagggc atggcgccgg
     2461 atggccacgt cgtcccggcc ctgcatgcca gcgacaaccg atgagaatct ccgggcgagg
     2521 gactcacccc tcgtagcgct cggtcatctc gcgggcgagg agatgctcgg gcaagagctg
     2581 gttggcgccg aagcgcaagg cgctgggtgt gccgctgtcg tcacggtaga gccgcacatt
     2641 ttcgccgatg tgtccgaatc cggagccgcg gacgatgcgc ccggcatcgt cgccggtcaa
     2701 ttcgatggtc gtaccgcctt cgaaaggctt gaacgacgcc gggaaagcaa gccgcggtga
     2761 agctcttgga ctgcgagccg atgcgaaagc ggtggcgcgg cgtcagcttc tcgccggtcg
     2821 cgagatccgc ggtcccgaag gcgcgctcga gaacgatccg gccatgctct gccacggcga
     2881 cgacgcaacc gggctgctcg agatggcgga gctgaaactc gagccagcgc gggatgtagt
     2941 cgagggcggc agaaagccat tcgctcaagg atcagctccg ggccatcgcg ggcaccgggg
     3001 cccgcgcgat ggcgtcgatt tcgagattgc tgcaaagtat cggggagact atgagagcgc
     3061 gatgcaggcg tccagtcagg cgaggatgcg gtgtcgcggc aaaaggaaat gttgcgtgag
     3121 attgcgcagg tgatcgccgc ccagggcgaa taccggatcg gcgaatggtt caccgaggat
     3181 ttcagactga tcgagccgac caagccgaac tggccgcgcg gacacgaggg tgcccgcaag
     3241 ctgctcgagg ctttcagaac gctcaggccg cccatcgaat tcgtcgccct cgacatggtc
     3301 gaggaaggcc accgcgtcgc cgtccgctgg cagctatcgg cgacccacca gggcgagcga
     3361 ttgcctctcg catcgatcgc gatctaccga ttcgagaagg gtctcatcgc cgaggattgg
     3421 ggcatcccca ttcgcgggga gtggctcgat tagtcggcgc gtgggagagg atgctgcgcg
     3481 ggcgcgaaaa gatgtggatt gaggcagaaa tcccctcacc ctcccgacgc tatcgcgtcg
     3541 ggcccctccc tctcccgcga tgcgggagag cggttgatcg tgcagtcgga tgagaggggc
     3601 ggtcgaggat gtcgcgcgtg gccggctcgc aggatttctc gccgccgcgg ccggcgcacg
     3661 gagggtcgcg atcgaagcgc tgcggttgtt gccggggggc gcggtccagg agaattggct
     3721 gatcgaggcg aggctcgccg gcgggccgct cgagggatgc caacgcttcg tgctgcgcaa
     3781 ggcggcgccg acgacgctga cgctcagcca cgatgctgcg acggagtttg cggtgctgcg
     3841 cgccgtgcag gacgcaggcg tcgccgttcc cgagccgctg ttcctctgcc gcgaccccgc
     3901 catgctgggc aagccgtttc tcgtcatgcg ctgggttccc ggtgtcgccg acggcgcgct
     3961 tctggcggcg gatgccgtca acgaagcgct ggccgagcgc ctcgggcggg agctggcgct
     4021 gctgcatcgg atcgtgccgc cgcgggccgg tctggcgggg ctcggtgcgc cgcccgtaga
     4081 cgcggccgcg gcgcggctgg cgcaatatcg cgacatgctt gacgcgtccg gcgatgcgca
     4141 tccggtggcg gagtggggga tgcgctggct gctccgccat gcgccgccgc ccgcggcgcc
     4201 ggtgctctgc catggcgact tccgcaccgg aaactacctc gtcgacgagg gccgcctcac
     4261 ggcggtgctc gactgggaat tcgcggggtg gggcgatccc gacgaggaca tcggctggtt
     4321 ctgcctggga tgctggcgct tcggcgctta tgagcgcggg gccggcggca tcgcgccgcg
     4381 cgcggctttc ctgcgcggct acatcgctgc ttcgggccgg cggctcgacc cggcgcggct
     4441 gcgctattgg gaagcgatgg cggcgctgcg ctggctcgtc atcgcgctcg gccagcgcga
     4501 ccgcttcctc agaggcggcg agcgctcgct cgatctcgcg ctcaccgggc ggcggccggc
     4561 cgaatgcgag ctcgagatcc tgcgcctcac cgcggaaccc gcctgatgcg cgaccggccc
     4621 gacggcacgc agttgctcgc cttggcgcgg gagctcgagg aaatcgcgcc gcgcctgccg
     4681 ccggaggagc gcgtggatcc cgcgctcctg gcgcgggcgc aggcgatcgc cgagcgcgag
     4741 cggcaggccg gcgacgcgcc gcttgcggcg tgccgcgagg ctctcgtcgc gctctatggg
     4801 cccggcgatt tcacggatct cttcgagcgc ctcgcgcgcg agatccgcgc cggcacttat
     4861 gacgcggcgg gagcggagcg cgaccgcgtc tgtcgagtgc tctgggccgt gacgctgcag
     4921 aagctgcgcg agagcaatcc tgaatatctt gcggcgagcg cgcgttagcg cgctcgtccg
     4981 cggcggcgac agcgattgcg caagcaatcg cgagagccgc acccggcagg agagtgagag
     5041 gtcgcgtgat ctaggctagc cggatgggtg cggctctcgc ggtttgcttt cgcaaaccgc
     5101 tggcgccgcc aaggattttt tggtcttagg cgttcgccta agaccaaaaa acctttgcgc
     5161 cgcgaacggc gtgggcggcg tcggggaagc gctggcggag gctctccatc tcatggccga
     5221 gccagccgag cacgagatgc tgcgtctcga gcggcaagcg caaggcggcg acgagcgcct
     5281 ggtcggccag cgcgtcgttc aggccgccca gcacgctttg cagcgtgacg agacgctcga
     5341 gataggccgc cgcgccgcgc tcgtcgaaca aggacttcaa taattccgcg ggatagcgga
     5401 gcttcttcac acgaagccgt aattcatgga acgcgatctg atcggcgagc tcgacctgct
     5461 tcactgcgcg gcgcagcttg cgatggcgct tctcgagcag ctccccggcg taatgcgcga
     5521 gcgggcgctc ccagccggaa gcgagcagat cggtcggccg gtgcagccag ggcgcctcct
     5581 cgacggcggc ctcgaccgcg gcgatgctcg ccttgagcgc gtcgccttgc ggcagcgcct
     5641 tgcggcgggc ggcgagcgcg cgctcgacga gcaggcccag cgccggatgc tccggcatcg
     5701 tctggcgcaa ctggccgacc gtgcgggcga ggaacacgtc ccattcgcgg acgcggccgt
     5761 a
//
LOCUS       DASYNS010000315         5928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4134180, whole genome shotgun sequence.
ACCESSION   DASYNS010000315 DASYNS010000000
VERSION     DASYNS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5928
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4134180"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VGP50_09700"
     CDS             <1..1044
                     /locus_tag="VGP50_09700"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HEV8027695.1"
                     /translation="AVAAPPASPSQPLAAAIVVNIPAAGSAPAPVAPAAAATAPVGSA
                     AAATTPVGTAAIGEQTKVRVRAGQPGDDADQDAAPAQATTQGPTDVAAGQQAKLAAGA
                     DPAVKPASDPSPDAANAVNATPPQATDDTTSPSQTQGTAGNGAGHAGTSTAGLSALSS
                     SEGGASGPSGTGTAKTTADGLPNFGFSLGATAPSSAPAANAATTSAAAVPITGLAVAI
                     AARAQAGSNQFEIRLDPPELGRIDVHLDVDRDGQVTSHVTADRADTLALLQSQQPQLE
                     RALEQAGLKTADNGLQFTLRDQSFAGQNDGGGNDPQPRVAQLVIPDTSAPSVDPTQIY
                     ARSGIGGGLDIRV"
     gene            1054..1794
                     /locus_tag="VGP50_09705"
     CDS             1054..1794
                     /locus_tag="VGP50_09705"
                     /inference="COORDINATES: protein motif:HMM:NF025230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HEV8027696.1"
                     /translation="MSTLSDTLSQVGTSALSQLGAASSATTPTTTPTTSTTASGTAAT
                     GMQSLAGNFDTFLQLLTTQLQNQDPLDPLDTNQFTQQLVEFASVEQQVNMNTNLQTLI
                     SLQQTTEATSALQFLGSTVTASGNTATLSNATGSPATWSLNAAAPATANITITNSSGQ
                     TAFTGTTTLNAGTQTFSWSGQGNSSVIWPDGNYTMTISAVGANGQPVTVSTQVQGVVT
                     GVNLSQNPPLLTVGGQNIALSQIQSISR"
     gene            1966..2241
                     /locus_tag="VGP50_09710"
     CDS             1966..2241
                     /locus_tag="VGP50_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEV8027697.1"
                     /translation="MTEPHRPRVKYVIGPDGSPLTIADLPAPGTKRWVIRRKAEVVAA
                     VRGGLLSLEEACSRYTLTVEEFLSWQYSIDQHGLAGLRTTRIQQYRQ"
     gene            2444..2836
                     /locus_tag="VGP50_09715"
     CDS             2444..2836
                     /locus_tag="VGP50_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027698.1"
                     /translation="MPGYSKTLLVALAGALGIVFCPPVLSQSNQEIRGTDAPAVAPAP
                     RTINLTQEQRFIIREIVLKDLNVAKARADAPETIGDAVPENIDLHPMPPELAAKVPQG
                     RSHTFFVKQDNAIVLVSPSDRRVADVIH"
     gene            2956..4617
                     /gene="fliF"
                     /locus_tag="VGP50_09720"
     CDS             2956..4617
                     /gene="fliF"
                     /locus_tag="VGP50_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HEV8027699.1"
                     /translation="MKGLIDFVRTLGAARIAAMGAVTITLVGFFAFLMLRVTAPQMTP
                     LFTDLTFEDSTAIVKELERQGVPYELRNDSSIVMVPKDRVARLRMGLAEGGLPKGGGV
                     GYEIFDKSDALGTTSFVQNINNLRALEGELARTIRGLDRVQAARVHLVMPERPLFSRD
                     KMEPSASIVVKVRGALEPQQVRAIRHLVASAVNGLKPQRVSIVDEAGHLLADGAGDDP
                     NGTEVSADERQAAFEKRLREQIETIVSSVVGPGRARVELTADFDFNRVTQTSDKYDPE
                     GRVVRSSQTREETSASNEGRENQVTVGNEIPGGNQRQAPAPEAAGTPRDQSRKSEEII
                     NYEISKTSKTEVIEGGRVNRISVAVLVDGTYGKNDKGDATYQPRSKEEIDQIGALVRT
                     AIGFDQKRGDQVQVINLRFAESPAVAPITEPSGWMSALQFTKDDLMRGIEMVVMALLG
                     LVVLLLVVRPLVRRILAPDERAVAALPPADASADAPLAPAIVAPAESVVAKAEPSQTA
                     KMIDIAQVQGQVHAQSVQKVGDLADKNPRETVSIIRQWLNESAAA"
     gene            4629..5729
                     /gene="fliG"
                     /locus_tag="VGP50_09725"
     CDS             4629..5729
                     /gene="fliG"
                     /locus_tag="VGP50_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEV8027700.1"
                     /translation="MPSAYADRSGVAAQLSNALASLSSSGRAANAPTSKRQLTGPERA
                     AVLMLALGEQHGSKVWGMLDDDELREISIVMSTIGTIEAELVENLLLEFVSRMSASGA
                     LMGTYDATERLLQQYLPPERVGNIMEEIRGPAGRNMWEKLSNVQEEVLANYLKNEYPQ
                     TVAVVLSKIGPEHAARVLAILPEELALDVVNRMLRMEAVQKEVLERVELTLRTEFMSN
                     LSQTRRRDAHEVMAEIFNNFDRQTETRFLTALEEENRESAERIKTLMFTFDDLTKLDT
                     ASAQTLMRHIEKDKLGVALKGAAEPVRQFFLSNMSTRAAKMLMDDMQAMGPVRLRDVD
                     EAQVLLVNLAKDLAAKGEIVISKSRGEEELVY"
     gene            5742..>5928
                     /locus_tag="VGP50_09730"
     CDS             5742..>5928
                     /locus_tag="VGP50_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027701.1"
                     /translation="MGAATKFLFDVDFAASDGKPERQAITLAEHALKLAEAETAAHRR
                     GYEDARTDAGIEAERRMA"
BASE COUNT         1117 a         2063 c         1874 g          874 t
ORIGIN      
        1 gcggtcgccg cgccgccggc cagtccatcg cagccgctgg cggccgctat tgtcgtcaac
       61 atccctgccg cagggtcagc cccggcgccg gttgcgcccg cagcggctgc cacggcgccg
      121 gtcgggtccg cagcggctgc cacgacgccg gttgggaccg cggcaatcgg cgagcagacg
      181 aaggtgcgcg tgcgcgccgg tcagcccggc gacgatgcgg accaggatgc ggcgccggca
      241 caagctacga cccaaggccc gaccgacgtc gcagccggcc agcaggccaa attggcggcc
      301 ggcgccgacc ccgcggtcaa gcccgcgtcc gatccctccc cggatgccgc gaacgccgtg
      361 aacgccaccc cgccgcaagc gaccgacgac acgacctcac caagccagac tcagggcact
      421 gccggcaacg gcgccggaca cgccggcaca tcgacggccg ggctttccgc actttcttcc
      481 tctgaaggcg gcgcaagcgg cccaagcggg accggcacgg cgaagacgac cgcagacgga
      541 ttgccgaatt tcggcttctc gctcggcgcc acggcgcctt cgtcagcgcc tgcagcgaac
      601 gccgccacga cctcggcggc cgcggtgccg attaccggcc ttgccgtcgc catcgcggcg
      661 cgggcacagg ccggctccaa tcagttcgag atccgtctcg atccgccgga actcggccgc
      721 atcgatgtgc acctcgacgt cgatcgcgac ggccaggtga cgtcgcatgt gaccgccgac
      781 cgcgccgata cgctggcgct gctgcaaagc cagcagccgc agcttgagcg cgcgctcgag
      841 caagccggat tgaagacggc cgataacggc ctgcagttca cgctgcgcga tcagtccttc
      901 gccggacaga acgatggcgg cggcaacgac ccgcagccga gagtggcgca gcttgtcatt
      961 cccgatacca gcgctccttc cgtcgacccg acgcaaatct atgcgcgctc cggcatcggc
     1021 ggcgggctcg acattcgcgt gtgaggatgg atcatgagca ctcttagcga cacgctttcg
     1081 caggtcggca cctcggcgct gtcccagctc ggcgccgcaa gctcggccac gacgccgacg
     1141 acgacgccga cgacctccac gaccgccagc ggcacggcgg ccaccggcat gcagtcgctc
     1201 gccggcaatt tcgacacctt cctgcagctc ctgaccaccc agctgcagaa ccaggacccg
     1261 ctcgacccgc tcgataccaa tcagttcacc cagcagctcg tcgagttcgc ctcggtcgag
     1321 cagcaggtca acatgaacac caacctgcaa acgttgatct cgctgcagca gaccacggaa
     1381 gcaacctcgg ccttgcaatt cctcggcagt acggtcaccg ccagcggcaa taccgccacc
     1441 ctctccaacg cgaccggcag cccggcgacc tggagcctca acgccgccgc tccggcgacg
     1501 gccaacatca cgatcaccaa ttcgagcggg cagacggcct tcaccggcac aacgacgctc
     1561 aacgccggaa cgcaaacctt cagctggagc ggccagggta acagcagcgt gatctggccg
     1621 gacggcaact acaccatgac gatcagcgcc gtcggcgcca acggccaacc ggtcaccgtc
     1681 tcgacgcagg tgcagggcgt cgtcaccggc gtcaatctga gccagaaccc gccgctgctg
     1741 acggtcggcg gacagaacat cgcgctcagc caaatccagt cgatcagccg ttaacgtctc
     1801 gccgcttcga ctccaggttc cgatcgccgc gcgccttcgc ccagcgaggc gcggcacacg
     1861 tccgctcggg ctaaagacgc caatcgaccg ttaggcttag gcaaatttta agccgcgctg
     1921 cgtaaggtcc aaagctcgta gtgtgtcgag ttcgtgagag ttcctatgac cgagccccat
     1981 cgcccgaggg tcaagtatgt catcgggccg gacggttccc ctctgaccat cgccgatttg
     2041 ccggcgccgg gaaccaaacg ttgggtcatt cgccgtaagg cggaagtcgt agccgcggtc
     2101 cgcggcgggc ttctttcgct cgaagaggcg tgcagccgct atacgctgac ggtcgaggag
     2161 ttcctctcct ggcaatattc gatcgaccaa cacggcctgg cgggcctgcg cacgacccgc
     2221 atccagcaat atcgccagta acgggcgctt aatcggtctt accgaattgc caatggccgc
     2281 tgccgcggcc tgttctgttg cgtgcgcgaa tcagtatgcg tgttgagcgg cttcgctcgc
     2341 agcccggtgg cggcgcgctg atggcgtccg ccaggcgcca tggaacccgg cggcggcccg
     2401 gcgcgttaac gatgaccctc ctctcacacg cggtgatttg cacatgccgg gatattcgaa
     2461 gacgctcctc gtcgccctcg ccggcgcact gggtatcgtg ttctgcccgc cggtgttgag
     2521 ccaaagcaac caggaaatcc gcggaaccga cgcgcccgcg gttgcaccgg cgccgcggac
     2581 catcaacctc actcaggagc agcgctttat catcagggag atcgtgctca aggatctcaa
     2641 cgtcgccaaa gcgcgggcgg acgcgccgga aacgatcggc gacgcggtgc cggagaacat
     2701 cgaccttcac ccgatgccgc ccgagcttgc ggcgaaggtg ccgcaaggcc ggtcccacac
     2761 cttcttcgtc aagcaggaca acgccatcgt cctggtcagt ccgagcgacc gccgcgtggc
     2821 tgacgtgatc cactgacggg catcgccagc ggctttatcg cccgccggcg gcctccatta
     2881 acggagtgct aactatagac cgggcaaata ttgccgagcg agccgcgtgg cgcgctcagc
     2941 gaggaaatcg gcccggtgaa aggtctcatc gacttcgtca ggacgctcgg cgccgcgcgc
     3001 attgccgcga tgggcgccgt caccattacc ctcgtcggct tttttgcctt cctgatgctg
     3061 cgcgtgaccg cgccgcagat gacgccgctt tttaccgacc tcaccttcga ggactccacc
     3121 gctatcgtga aggagctgga gcgccagggc gttccttacg agctgcgcaa cgacagcagc
     3181 atcgtcatgg tcccgaagga ccgtgtggcg cggctgcgca tgggccttgc cgagggcggc
     3241 ctgcccaagg gcggcggcgt cggctacgag atcttcgaca aatccgacgc gctgggcacg
     3301 accagcttcg tacaaaatat caataatttg cgcgcgctcg aaggagagct cgcacgcacc
     3361 attcgcgggc tcgaccgggt gcaggccgcg cgcgtgcatc tggtaatgcc agagcgtccg
     3421 ctgttctcgc gcgacaagat ggagccttcc gcatcgatcg tggtcaaggt tcggggcgcg
     3481 ctcgagccgc agcaggtgcg cgccatccgc cacctcgtcg cctccgccgt caacggatta
     3541 aagccgcaac gggtatcgat cgtcgacgaa gccgggcatc tcctggccga cggcgccggc
     3601 gacgatccga acggcaccga ggtcagcgcc gacgagaggc aggcggcgtt cgagaaacgg
     3661 ttgcgcgagc agatcgagac gatcgtctct tcggtcgtcg ggcccggccg cgcccgcgtc
     3721 gagctcaccg ccgatttcga cttcaaccgg gtcacccaga cgtcggacaa atacgatccg
     3781 gaaggccgcg tcgtgcgctc gagccagacg cgcgaggaaa cctccgcctc gaacgagggc
     3841 cgggagaatc aagtcacggt cggcaacgaa atacccggcg gcaaccagcg ccaagcgccg
     3901 gcgccggagg ccgccggcac gccgcgtgac caaagtcgga aatccgagga gatcatcaac
     3961 tacgagatct ccaagaccag caagaccgaa gtgatcgaag gcggccgcgt caaccgcatc
     4021 tcggtcgccg tcctggtaga cggtacctac gggaagaacg acaagggcga tgccacctat
     4081 cagccgcgca gcaaggagga aatcgatcag atcggagcgc tcgtgcgcac cgcgatcggc
     4141 ttcgaccaaa agcgcggcga ccaggtccag gtcatcaatt tgcgctttgc cgagtcgccc
     4201 gccgtcgctc cgatcaccga gccgagcggc tggatgtcgg cgctgcaatt caccaaggac
     4261 gaccttatgc gcgggatcga gatggtggtc atggccctcc tcggcctggt cgtgctgctg
     4321 ctggtggtgc ggccgctcgt tcgccgcatc ctcgctccgg acgagcgcgc cgtggcggca
     4381 ttgccccccg ccgatgcttc cgccgacgcc cccctggcgc cggcgatcgt cgccccggcg
     4441 gaaagcgtgg tggcgaaggc ggagccgagc cagaccgcaa agatgatcga catcgcccag
     4501 gtccagggcc aggtgcacgc ccaatcggtg cagaaggtcg gcgaccttgc cgacaagaat
     4561 ccccgcgaaa ccgtttccat catccgccaa tggctcaacg agagcgcagc cgcgtgaacg
     4621 cttgaccgat gccttccgct tacgccgata gaagtggagt cgccgcgcaa ctcagcaacg
     4681 cgttggcgag cctttccagc tccggccgcg cggcgaacgc gccgaccagc aagcgccagc
     4741 tcaccggccc cgagcgcgcc gccgtgctca tgctggcgct cggcgagcag cacggctcca
     4801 aggtctgggg catgctcgat gacgacgagc tgcgcgaaat ctcgatcgtc atgtcgacca
     4861 tcggcacgat cgaggcggag ctggtcgaga acctgctgct cgagttcgtc tcgcgcatgt
     4921 cggcctccgg cgcgctcatg ggcacctatg acgcgaccga gcgcctgctg cagcaatatc
     4981 tgccgccgga gcgggtcggc aacatcatgg aggagatccg cggcccggcc ggccgcaaca
     5041 tgtgggagaa gctctccaac gtgcaggagg aggtgctcgc caactatctc aagaacgaat
     5101 atccgcagac ggtcgccgtc gttctttcca agatcgggcc ggagcacgcc gcccgcgtgc
     5161 tcgccatcct gcccgaggag ctcgccctcg acgtggtcaa ccgcatgctg cgcatggagg
     5221 cggtgcagaa ggaagtgctc gagcgcgtcg agctcacgct gcgcaccgag ttcatgtcga
     5281 acctctcgca gacccgccgg cgcgacgcgc acgaggtgat ggccgagatc ttcaacaatt
     5341 tcgaccggca gacggaaaca cgcttcctca ccgcgctcga ggaggagaac cgcgaaagcg
     5401 ccgagcgcat caagacgctg atgttcacct tcgacgatct gaccaagctc gacaccgcct
     5461 cggcgcaaac gctcatgcgc catatcgaga aggacaagct cggggtcgcg ctcaagggcg
     5521 cggccgagcc ggtgcggcaa ttcttcctgt ccaacatgtc gacccgcgcc gccaagatgc
     5581 tgatggacga catgcaggcg atggggccgg tgcggctgcg cgacgtcgac gaagcgcagg
     5641 tgctgctcgt caatctggcc aaggacctcg ccgccaaggg cgagatcgtc atctccaagt
     5701 cgcgcggcga agaagaactg gtctactgag gaacggacag gatgggcgca gcaacaaaat
     5761 tcctgttcga tgtcgatttc gccgcgagcg acggcaagcc ggagcggcag gcgatcacgc
     5821 tcgccgagca tgcgctgaag ctcgccgagg cggaaaccgc agcgcaccgc cgcggctacg
     5881 aggacgccag gaccgacgcc gggatcgagg cggagcggag aatggccg
//
LOCUS       DASYNS010000316         2988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_544364, whole genome shotgun sequence.
ACCESSION   DASYNS010000316 DASYNS010000000
VERSION     DASYNS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544364"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2644
                     /locus_tag="VGP50_09735"
     CDS             <1..2644
                     /locus_tag="VGP50_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV8027702.1"
                     /translation="RSLSFIEDHDLLDPQRPNRNPAIEWRRFAMAALGRAGGTFDHSL
                     KSGGGSTLATQIEKFRHSPAGRTEGVVEKLLQMASATARAYLDGPETLLAQQRILTTY
                     LNATPLSSRPGYGEVIGLADGLAAWYGTDFVEANHALASPPPEDVTPARRAEIYKQAL
                     SLLIAERRPSWYLNGGRAELEGVTNGYLRRLAAADVIEPRLRDDALAVKLHVALSPPP
                     LPSSSFIERKAVDAVRGELMTALGASDLYGLDRMDIVADSTIDATTQGRVADKLAQLD
                     SPEVVRALGLVGDKLLGGADPGRVAYSVVLYERGADRNYVRVHADSLDQPFDINSGAK
                     MILGSTAKLRTLATYLGIVTDLHDELAPSPLPKLRHMAATSEDPLRRWAAEYLTGTSD
                     RSLQPMLDAAMQRRYSGSPGEEFFTGGGVHVFHNFERNEDYQAPTVEDAFAHSVNLSF
                     IRLLRDVVRHYEAEGSESESDSDGAAVADRDSSAREDYLRRFADQEGRVYLGRYYNEY
                     RSLSPDAALAHLAARAHGLPRRLAVVYRTVRPEASPAEFQAFLAAALPGTEFDDDDVA
                     ELYEKYAPKLFPLNDRAFLARVNPLELWTVAYLQQRPAASRGEVMDASAAIRQEAYSW
                     LFKTHNARKQDIRIRTLREEDAFDRMLQDWHRQGYPFDHLVPSLATAIGSSGDRPDAL
                     AQLMGIIVNDGVRLPTTDLQRLQFAAGTPYETVMGYEPPAPVRVMAPEVASTIRRALT
                     GVVATGTATRLKGAYAASDGALLPVGGKTGTGDNRFETFSNGHALVSSRVVDRTATFV
                     FFLGDRFFGTVTAYVPGRDAADYHFTSALAVSLLKALSPELTPLIATPQAEASLGEIP
                     QPRAALSTVSTSVALPAPPATKPN"
     gene            complement(2646..>2988)
                     /locus_tag="VGP50_09740"
     CDS             complement(2646..>2988)
                     /locus_tag="VGP50_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV8027703.1"
                     /translation="VAGCAPDEMGIGPVFAVPKLLEKHGLKIDDIDLWELNEAFACQV
                     IYCRDRLGIPHDKLNVNGGAISIGHPYGMSGARMTGHILIEGKRRGIKYGVVTMCIGG
                     GMGAAGLFEIF"
BASE COUNT          483 a         1104 c          937 g          464 t
ORIGIN      
        1 acgctcgctc tccttcatcg aggaccacga cctcctcgat ccgcagcgcc ccaaccggaa
       61 cccggcgatc gagtggcggc gtttcgccat ggcggcgctc ggacgcgccg gcggcacctt
      121 cgaccacagc ctcaagagcg gcggtggcag cacgctcgcg acgcagatcg agaaattccg
      181 tcactcgccc gccggccgca cggagggcgt ggtggagaag ctgctgcaga tggcgtccgc
      241 cacggcgcgg gcctatctcg acggaccgga gacgctcctc gcccagcagc ggattctcac
      301 cacgtacctg aacgccacac ccttgagctc aagacccggc tatggcgagg tgatcgggct
      361 ggccgacggg ctcgccgcct ggtacggaac cgacttcgtc gaggcgaatc atgcgctggc
      421 atcgccgccg cccgaggacg tcacgccggc gcggcgcgcc gagatttaca agcaagccct
      481 cagtctgctg atcgccgagc gccgcccgtc ctggtacctg aacggcggcc gcgccgagct
      541 cgaaggagtg accaacggct atctgcgccg cctcgccgcg gcggacgtga tcgagccgcg
      601 gctgcgcgac gatgcgctcg cagtcaagct gcatgtcgcc ctgtcaccgc cgccgctgcc
      661 gtcctcctct ttcatcgagc gcaaggccgt cgacgccgtg cgcggcgagc tgatgacggc
      721 cctcggcgcc agcgatctct atggtctcga tcggatggat atcgtcgccg actcgacgat
      781 cgacgcgacg acgcagggcc gcgtcgccga caagctggcg cagctcgaca gccccgaggt
      841 ggtgcgcgcg ctcggcctcg tcggcgacaa gctgctcggc ggcgccgacc ccggacgcgt
      901 cgcctatagc gtcgtcctct acgagcgtgg cgccgaccgc aactatgtgc gcgtccatgc
      961 cgacagcctc gatcagccct tcgacatcaa ttccggcgcg aagatgatcc tgggctcgac
     1021 cgccaagctc cggacgctcg cgacatatct cggcatcgtg acggacctcc acgacgaact
     1081 ggcgccgagc ccgctgccga agctgcggca tatggcggcg acgagcgagg acccgctgcg
     1141 ccgctgggcg gccgaatatc tcacggggac gagcgaccgc agcctccagc cgatgctcga
     1201 tgcggcgatg cagcgccgct actcgggtag tcccggcgag gagttcttca ccggcggcgg
     1261 tgtccacgtc ttccacaatt tcgagcgcaa cgaggattat caggcgccga ccgtcgagga
     1321 tgcgttcgcg cattcggtga acctctcctt catccgcctg ttgcgcgacg tcgttcgcca
     1381 ttacgaggcc gaaggcagcg agagcgaaag cgacagtgac ggcgccgccg tcgccgaccg
     1441 cgatagcagc gcccgcgagg attacctgcg tcgctttgcc gatcaggaag gccgcgtcta
     1501 tctcggccgc tactacaacg agtaccgcag tctttcgccc gacgcggcgc tggcccatct
     1561 cgcggcccgc gcccacggcc tgccgcgccg cctcgccgtc gtataccgca ccgtccggcc
     1621 cgaggcatcg ccagcggagt tccaggcctt cctcgctgcg gcgctgcccg gcaccgagtt
     1681 cgatgacgac gatgtcgccg agctttacga gaaatacgcg cccaaactct tcccgctcaa
     1741 cgaccgcgcc ttcctcgccc gcgtcaatcc gctcgagctc tggaccgtgg cctatctgca
     1801 gcagcgtccc gcggcctcgc gcggcgaggt catggatgcg agcgccgcga tccgccagga
     1861 ggcctattcc tggctcttca agacgcacaa cgcccggaag caggacatcc gcatccgcac
     1921 gctgcgcgag gaagacgcgt tcgaccggat gctgcaggat tggcaccgcc agggctatcc
     1981 cttcgatcac ctcgtgccgt cgctggcgac cgcgatcggc agctccggcg accggcccga
     2041 tgcgctggcc cagctcatgg gcatcatcgt caatgacggc gtgaggctgc cgacgaccga
     2101 cctgcagcgc ctgcaattcg ccgccggaac gccttacgag acggtgatgg gctatgagcc
     2161 gcccgccccg gtgcgggtca tggcgcccga ggtcgcgtct accatccgcc gcgcccttac
     2221 cggcgtggtc gccaccggca ccgcgacgcg cctcaaaggc gcctatgccg cgagcgacgg
     2281 cgcgctgctt ccggtcggcg gcaagaccgg caccggcgac aaccggttcg agaccttcag
     2341 caacggccat gccctcgtct cgtcgcgcgt cgtcgaccgc acggcgacct tcgtcttctt
     2401 cctgggggac cgcttcttcg gcacggtcac cgcctatgtt ccgggccgtg acgccgccga
     2461 ctatcacttc accagcgcgc tcgccgtgtc gctgctgaag gcgctctcgc ccgagctcac
     2521 gccgctcatt gcgacgccgc aggccgaggc gagcctcggc gagataccgc agccgcgcgc
     2581 cgcgctcagc accgtctcga ccagcgtcgc cctccccgcg ccgcccgcga ccaagccgaa
     2641 ctgacctaga aaatctcgaa cagtcccgcc gcccccatgc cgccgccgat gcacatggtg
     2701 acgacgccgt atttgatgcc gcggcgcttg ccttcgatga ggatatggcc ggtcatgcgc
     2761 gcgcccgaca tgccgtaagg gtggccgatc gagatcgcgc cgccattgac gttgagcttg
     2821 tcgtgcggaa tgccgagacg gtcgcggcag tagatgacct ggcaggcaaa cgcctcgttg
     2881 agttcccaaa gatcgatgtc gtcgatcttc aacccgtgct tttctaataa cttcggcacg
     2941 gcaaagaccg ggccgatacc catctcgtca ggcgcgcagc cggcgacc
//
LOCUS       DASYNS010000317        11183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3198185, whole genome shotgun sequence.
ACCESSION   DASYNS010000317 DASYNS010000000
VERSION     DASYNS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11183
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3198185"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VGP50_09745"
     CDS             <1..343
                     /locus_tag="VGP50_09745"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEV8027704.1"
                     /translation="ILGAMQVAENGDLANWMIPGKMVKGMGGAMDLVAGVKKVVVVME
                     HTAKDEPKLLKRCNLPLTGAGVVDMVITDLGVFAIDKKGHSGMALIELAPGVTFDEIR
                     VKTEASFRLKN"
     gene            455..1024
                     /locus_tag="VGP50_09750"
     CDS             455..1024
                     /locus_tag="VGP50_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEV8027705.1"
                     /translation="MTKGLSGKKVAILATDGFEQVELTKPRDALKSEGATVEIVAPHG
                     GEIQGFKHLDKGDKVTVDRTLDEARPDDYDAIMLPGGVANPDQLRMLPKAVAFARHFY
                     DAQKPIAVICHGPWTLIDAGVVKDLEITSWPSLKTDLKNAGARWVDRDVVVNSGVVSS
                     RRPDDLPVFCKKMLEEFREGRHIRRQAAE"
     gene            complement(1204..2088)
                     /gene="folD"
                     /locus_tag="VGP50_09755"
     CDS             complement(1204..2088)
                     /gene="folD"
                     /locus_tag="VGP50_09755"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEV8027706.1"
                     /translation="MVARLIDGKAEAAALRARVAAAAAELKAQHGFVPGLAAVLVGDD
                     PASDIYVRGKGKAAIAAGIASFEHRLPASTSERQLLDLVLRLNNDPAIDGILVQLPLP
                     PQIAPQRVIETVDPAKDVDGFHPQNVGRLWSGGRALVPGTPYGCLMLLQATLGSLAGA
                     EAVVIGRSNIVGKPMAALLLAENCTVTLAHSKSRDLPLIVRRADIVVAAAGRAELVRG
                     AWIKPGATVIDVGTTRLPAEGGKTRLVGDVAFAEAQAVAGAITPVPGGVGPMTIACLL
                     RNTAIAACRRRGLAGPAL"
     gene            complement(2073..2399)
                     /locus_tag="VGP50_09760"
     CDS             complement(2073..2399)
                     /locus_tag="VGP50_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEV8027707.1"
                     /translation="MVSPFARAADGVRLAVRLAPKASAERILGLADEADGGVALKIAV
                     TAAPEAGKANDALLAFLARLLRVPKRDLTLAQGASDRRKLVHVAGDPALLAQRLEEAL
                     GPWSRG"
     gene            complement(2393..2698)
                     /locus_tag="VGP50_09765"
     CDS             complement(2393..2698)
                     /locus_tag="VGP50_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV8027708.1"
                     /translation="MGRFLSPVIEVIILAIDIYWWIIIVSAVMSWLIAFNVINTYNRT
                     VAMIGDFFYRATEPALRPIRRFLPNLGGLDISPIILLLILWFVERELIELAFYVRQW"
     gene            2821..2896
                     /locus_tag="VGP50_09770"
     tRNA            2821..2896
                     /locus_tag="VGP50_09770"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2854..2856,aa:Ala,seq:cgc)
     gene            complement(2980..3411)
                     /locus_tag="VGP50_09775"
     CDS             complement(2980..3411)
                     /locus_tag="VGP50_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV8027709.1"
                     /translation="MNVEAILRDKGRNVTTIAPDASIGEAAALLRRLGIGALVVSRDN
                     VGVDGILSERDIVHGLADRGAALLDVKVEQLMTRRVSTCTPRESIGDLMSMMTERRIR
                     HIPVLRDGALVGIVSIGDVVKHRLDEMEYETSSLRSFIAGA"
     gene            3790..4317
                     /locus_tag="VGP50_09780"
     CDS             3790..4317
                     /locus_tag="VGP50_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV8027710.1"
                     /translation="MRPIAIAVLLAAFLATASAPRPGLAEQAQPSEEAHWPAVAQKKW
                     PPNNSMPRHHVAMAWGVPAPYAQASNPLERSSATVRRGAKVYAAHCVSCHGPSGQGDG
                     KAGRNLSPPAGNLAWLAQLPASRWDPYIYWTVIEGGSAFGTAMPAFKDALSNDDIWAV
                     IAYVEARLPPKPKSR"
     gene            complement(4314..5525)
                     /locus_tag="VGP50_09785"
     CDS             complement(4314..5525)
                     /locus_tag="VGP50_09785"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV8027711.1"
                     /translation="MGGTVKKIVLAYSGGLDTSVILRWLQETYECEVVTFTADLGQGE
                     ELEPARKKAELLGVKQIFIEDLREEFVRDFVFPMFRANALYEGQYLLGTSIARPLIAK
                     RQIEIARQVGADAVAHGATGKGNDQVRFELSYYALQPHIKVIAPWREWDLASRTKLVE
                     YAETHQIPIPRDKHGEAPFSTDSNLLHISAEGKVLEDPWIEPEEFVYSRTVAPEAAPD
                     KPEYVEIEFRRGDPIAVDGAALSPAQLLAKLNELGGQHGIGRLDLVENRFVGMKSRGV
                     YETPGGTILHAAHRGIESITLDRGAAHLKDELMPRYAELIYNGFWFAPEREMLQALID
                     KSQDNVEGTVRLKLYKGSAHVVGRKSPKSLYSLAHVTFEADQVYDQRDAQGFIKLNAL
                     RLRLAKALRKN"
     gene            5685..6257
                     /locus_tag="VGP50_09790"
     CDS             5685..6257
                     /locus_tag="VGP50_09790"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEV8027712.1"
                     /translation="MKRTVRYVSAVSAVMLLPILGGQAAQAAEILTLKSPALKDNGVL
                     AKKYAGNDTSNPNCVGENISPPLGWSHPPEGTKSFALIMYDPEGRSGLGVVHWVAYGI
                     PAEVTGFAEGEVSKPSDKFVGGKSTRGLATYFGPCTPPGDWHHYTFTLIATDLDPKAL
                     QPGMTRDELLAALQGHGKGAAGLIGRFKHK"
     gene            complement(6411..7310)
                     /locus_tag="VGP50_09795"
     CDS             complement(6411..7310)
                     /locus_tag="VGP50_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8027713.1"
                     /translation="MSWQNVVLSQLLRWRTKPLSKREPDPARAREFVAKTFRDPLVPK
                     GWRVRQGTDAALAGDWIEPADGAPAARTLLYFHGGGYCFCSPATHRPITFALATGADA
                     RAVAPVYRLAPEHRFPAAVEDAVAAYRRLVALGTPPSRIVVGGDSAGGGLALAALLSL
                     RDAGDTLPAGAVLFSPWTDLAATGASLVTNDRRDVMFHGYSVAKGARVYLGDAPATHP
                     LASPLYGDLRALPPLFVQVSDSEVILDDSTRLAAKATAAGVTVDLKQWHRLPHVWQIF
                     APRLPEARAALAEAAAFIRRVTP"
     gene            7385..8422
                     /locus_tag="VGP50_09800"
     CDS             7385..8422
                     /locus_tag="VGP50_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HEV8027714.1"
                     /translation="MMTAATTMRQVWIPRHGPPEVLEVRDAPIPEPKSGEVLVKVAAA
                     GINFADIMARLGLYPDAPKPPCVVGYEVAGEVMALGPSASGVRAGDRVIAMTAFGGYS
                     SHVSVPQAQVFPLPAGMTLEQGAALPVTYLTAYQLMIAMGRLRSGESVLVHSAAGGVG
                     LSAIEIAAIIGVKVIGVASAAKHEYLRSRGVMGLIESSSSDLPSRVKELTAGRGVDLA
                     LDARGGSSWRQSYDSLAPTGRLAMFGLSTAVGGNPWLGTARALLGVPWLRFNPLTLMS
                     DNRGIFGVNIGHLWGHQELLGEWMRQILAWQSQGKIAPVVARSFRFDEAAAAHRYIEN
                     RSNVGKVLLVP"
     gene            complement(8433..9260)
                     /locus_tag="VGP50_09805"
     CDS             complement(8433..9260)
                     /locus_tag="VGP50_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8027715.1"
                     /translation="MQKLEGAVAVVTGAASGIGRSLALRLARAGCQLALADIDAGGLG
                     ETVAAARQAGSRATAHRVDVSALDQMTAFRDTVLGEHGGVDLLINNAGVALLGTVEEV
                     SLDDIRWLVDINFWGVVHGAKLFLPALKQRPEAHIVNISSVFGLIAPSGNAAYCASKF
                     AVRGFSESLAHELAGSNVRVSTVHPGGIRTPIVTHARAAAGVGNAQRDDVITRFEQAA
                     RTTPDAAAERILRGIRRNEKRILIGGDARFLDGLQRLLPVRYWNVLGRLFPSDAAPP"
     gene            9444..10079
                     /locus_tag="VGP50_09810"
     CDS             9444..10079
                     /locus_tag="VGP50_09810"
                     /inference="COORDINATES: protein motif:HMM:NF025338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8027716.1"
                     /translation="MKTRDRILKTALALFNESGEAGVSLAQIAAALDISEGNLWYHFR
                     TKRDLIAALLDELEQTIEQQHERPPADPGNVSTYADYSRQCFRTMWAYRFLYRARFDP
                     GKDRELAERRLAITVRGHHHSERHLTEMARCKLLDASPSEIAELVTADRIVSRYWFDY
                     LQERYGLVQLSEADLQSGVKQIFALYRPYLSAAARAQIAAAMPPGRKAARR"
     gene            complement(10112..>11183)
                     /locus_tag="VGP50_09815"
     CDS             complement(10112..>11183)
                     /locus_tag="VGP50_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8027717.1"
                     /translation="NNLLTAIAGNLDLVAAGIGNNQSLHRLIDAAQRAAQRGGRLTQQ
                     LLAFARQQTLRPQIVDLNTLIQEFRIIVNRAAGETIDVTFNPEGRLWPALVDPAQFQS
                     ALLNLVVNARDAMPDGGQIVIETRNVRIRNDAAMPELASGQYVMLAVSDNGIGMTTEI
                     VGRAFDPFFTTKEIGKGTGLGLSQVYGFARQSEGDAAIESAPGVGTTVRIYLPRAEGV
                     VDMDNAAGSMPESRNAQATILVVEDNAPVREMVVSSVTALGYRTLVAGTAREAMSILL
                     SGKPVDLMFSDIVMPGGMSGVELAREAGTLRPSLKILLTTGYAAVGREAALGDHGPFP
                     ILNKPYRMADLIEKLREVIGAS"
BASE COUNT         1876 a         3698 c         3812 g         1797 t
ORIGIN      
        1 gatcctgggc gcgatgcagg tggcggagaa tggcgatctc gccaactgga tgatcccggg
       61 caagatggtg aagggcatgg gcggcgccat ggacctcgtc gccggcgtca agaaggtcgt
      121 ggtggtgatg gagcacaccg cgaaggacga gcccaagctg ttgaagcgct gcaacctgcc
      181 gctcaccggg gccggcgtcg tcgacatggt gatcaccgat ctcggcgtct tcgccatcga
      241 caagaaaggc catagcggca tggccctgat cgagctcgcg cccggcgtca ccttcgacga
      301 gatccgcgtc aagaccgagg cgtcgttccg gctgaagaac tgaacgcgcc cgggatgacc
      361 tcgccggacg cggcggcaac tccaggcagc gctcggaaca tcggcgactc cgcattgttg
      421 ggaggagaag tttcccagac tgacggaggt cgacatgact aagggtcttt cgggcaagaa
      481 ggtcgcgatc ctcgcgacgg acggtttcga gcaggtcgag ctcaccaaac cgcgggacgc
      541 gttgaaaagc gaaggcgcca ccgtcgagat cgtggcgccg catggcggcg agatacaggg
      601 cttcaagcat ctcgacaagg gcgacaaagt gacggtcgat cgcacgctcg acgaagcaag
      661 gcccgacgac tacgacgcga tcatgctgcc gggtggcgtc gccaaccccg accagctccg
      721 catgttgccg aaggcggtgg ccttcgcgcg ccatttctat gacgcgcaga agccgatcgc
      781 cgtgatctgc cacggtccgt ggacgctcat cgacgccggc gtcgtcaagg acctcgagat
      841 cacgtcctgg ccgtcgctca agaccgacct caagaacgcc ggcgcgcgct gggtcgaccg
      901 ggacgtggtc gtcaacagcg gcgtcgtcag cagccgccgg cccgatgacc tccccgtctt
      961 ctgcaagaag atgctggagg agttccgaga aggtcgtcac atccgtcgtc aggcggcgga
     1021 gtgatcgctc actgatcaag cgcaacaact atcgatcgat ttttctctgc cagttccgag
     1081 agtcgtgtgc gggcggaacg aaaacgtacc gcccgcacca acgagttcgc gacccaggcc
     1141 agcaggcccg acgcgttcat ggccgtgatg ctggcgctca ggttcgagga gcgcctgagc
     1201 gggtcagagc gccggcccgg cgaggccgcg gcggcggcag gcggcgattg ccgtgttgcg
     1261 cagcaggcag gcgatggtca tcggcccgac gccgcccggc accggcgtga tcgcgccggc
     1321 gaccgcctgc gcctcggcga aggcgacgtc gccgacgagc cgcgtcttgc cgccctcggc
     1381 tggtagccgc gtcgtgccga catcgatcac ggtggcgccg ggcttgatcc aggcgccgcg
     1441 caccagctcc gcccgtcccg ccgccgccac cacgatgtcg gcgcggcgca cgatcaacgg
     1501 cagatcgcgg ctcttggaat gggcgagcgt gacggtgcag ttctcggcca gaagcaacgc
     1561 cgccatgggc ttgccgacga tgttcgagcg gccgatcacc accgcttcgg cgccggcgag
     1621 gctgcccagc gtcgcctgca gcagcatgag gcagccatag ggcgtgcccg gcaccaacgc
     1681 gcgtccgccg ctccagaggc gcccgacatt ctgcggatgg aagccgtcga cgtccttggc
     1741 gggatcgacg gtctcgatca cgcgctgcgg cgcgatctgg ggcggcagcg ggagctgcac
     1801 gaggatgccg tcgatcgcgg gatcgttgtt gaggcgcagc acgagatcga gcagctgccg
     1861 ctcgctggtg gacgccggca ggcgatgctc gaagctggcg atcccggcgg cgatcgccgc
     1921 cttgcccttg ccgcgcacat agatgtcgct cgcgggatcg tcgccgacga gcacggcggc
     1981 aagcccgggc acgaagccgt gttgcgcctt gagctcggcc gccgccgccg cgacgcgcgc
     2041 gcgcagcgct gccgcttcgg ccttgccgtc gatcagccgc gcgaccatgg gccgagggcc
     2101 tcctcgagcc gctgcgccag cagcgccgga tcccccgcca cgtgaacgag cttgcggcgg
     2161 tcgctggcac cctgcgccag cgtcaggtcg cgcttgggca cgcgcaacag gcgcgcgaga
     2221 aatgccagga gcgcgtcatt cgctttgccc gcttcgggcg cggcggtcac cgcgatcttg
     2281 agcgcgacgc cgccatccgc ttcgtcggca aggccgagga tccgctcggc tgacgccttg
     2341 ggcgccagcc gcaccgcgag gcgcacgccg tccgcggcgc gcgcgaacgg actcaccatt
     2401 gcctgacgta gaacgcgagc tcgatgagct cccgctcgac gaaccagagg atcaacaaga
     2461 ggatgatcgg cgagatgtcg agcccgccga ggttgggcag gaatctccgg atgggccgga
     2521 gcgccggctc ggtggcgcga tagaagaagt cgccgatcat cgccaccgtc cggttgtagg
     2581 tgttgatgac gttgaacgcg atcagccagc tcatcaccgc cgagacgatg atgatccacc
     2641 aatagatgtc gatcgccaag atgatcactt cgatcaccgg actgaggaat ctgcccatgg
     2701 ttcggttgga gcccccttgc gaagcttcgg cacggtagcg gcgtgaccaa ggccgtgcaa
     2761 gcgcggccgg cccaaccttg acaccccgcc atcgccgctt ccatagtcgc cgccgctcgc
     2821 ggggcagtag ctcagttggg agagcgcgac gttcgcaatg tcgaggtcag gggttcgatc
     2881 cccctctgct ccaccaaatc aaagggcccg gccggccaac ggccgggccc tttatttccg
     2941 tgatttccct ctcccgctcg cgggagacgc attcctcgct caggcgccgg cgataaaact
     3001 cctgagcgag ctcgtctcgt attccatctc gtcgaggcga tgcttcacca cgtcgccgat
     3061 gctgacgatg ccgacgagcg cgccgtcgcg cagcaccggg atgtgccgga tgcggcgctc
     3121 ggtcatcatc gacatcagat caccgatgct ctcgcgcggc gtgcaggtgg agacgcgccg
     3181 cgtcatgagc tgctccactt tgacatcgag cagcgccgcg ccgcggtcgg cgaggccatg
     3241 gacgatgtcg cgctcggaaa gaatgccgtc gacaccgaca ttgtctcgac tcaccaccag
     3301 ggcgccgatg ccgaggcgcc gcaacagcgc ggccgcctcg ccgatgctgg catcgggcgc
     3361 aatcgtcgtc acgttccgtc ccttgtcgcg tagaattgct tcaacattca tatgcgcctc
     3421 ctccaattgg cgcgggcgcc tgtcgccggt gcggcgcagg ccgccctttc tgagcgccag
     3481 tctacgacga aatcgccgtc ccgacggaga aaaaccgcgc gccacgccga ggacccgaat
     3541 gaattcgcgt cgtcaggcat accggggcga gaccgaaaat ttggtcgccg gcgcgcgagc
     3601 acgatgggca attgacgacg aatcatggac actattaacg atcgcgcgaa ccgggcggat
     3661 cgcgattcac gattgtgcaa tgcggcatgc agtaagacat tgccgcagag ccgatcgctg
     3721 cgcaggatgc cctcgggccg cctgagccgc tcttgctctc gttgctgctg cgtcgcggga
     3781 gaaacccagg tgaggccgat cgctatcgca gtgctccttg ctgcatttct tgccacggcc
     3841 agcgcgcccc gtcccggcct ggccgagcaa gcccagcctt ccgaggaagc gcattggccg
     3901 gccgtcgcgc agaagaagtg gccgcccaac aacagcatgc cgcggcatca tgtggcgatg
     3961 gcgtggggtg ttcccgcccc ttatgcccaa gcgagcaatc cgttggagcg aagcagtgcg
     4021 accgttcgcc ggggtgccaa ggtctacgcg gcacattgcg tctcatgcca cggcccaagc
     4081 gggcaaggcg acggcaaggc ggggcgcaat ctctcgccgc cggcgggcaa cctcgcctgg
     4141 ctcgcgcaat tgccggcgtc gcgttgggat ccctacatct attggacggt cattgaaggc
     4201 ggcagcgcct tcggcacggc gatgccggcc ttcaaggacg cgctgtcgaa cgacgatatc
     4261 tgggccgtca tcgcctatgt cgaggcgcgc ctgccgccca agcccaagag tcgctagttt
     4321 ttgcgcagcg ccttcgccag gcgcagacgc agcgcattga gcttgatgaa gccttgggcg
     4381 tcgcgctggt catagacctg gtcggcctcg aaggtgacat gagcgaggct gtagaggctc
     4441 ttgggcgact tgcggccgac cacgtgggcg ctgcccttgt agagcttgag ccgcaccgtg
     4501 ccttcgacat tatcctggct cttgtcgatg agggcctgaa gcatctcgcg ctcgggcgcg
     4561 aaccagaagc cgttatagat gagctcggcg tagcgcggca tgagctcgtc cttgagatgc
     4621 gcggcgccgc ggtcgagcgt gatgctctcg atgccgcgat gcgccgcatg caaaatggtg
     4681 ccgccggggg tctcgtagac gccgcgcgac ttcatgccga cgaagcgatt ctcgacgaga
     4741 tcgagccggc cgatgccgtg ctggccgccg agctcgttga gctttgccag aagctgcgcc
     4801 ggcgacagcg ccgcgccgtc gacggcgatc gggtcgccgc ggcggaactc gatctcgaca
     4861 tactcgggct tgtcgggcgc ggcctcgggc gccaccgtgc ggctatagac gaattcttcg
     4921 ggctcgatcc agggatcctc gaggaccttg ccctcggccg agatgtgcaa aagattggag
     4981 tcggtcgaga agggcgcctc gccgtgcttg tcgcgcggga tcggaatctg gtgcgtctcg
     5041 gcatattcga cgagcttggt gcgcgacgcg agatcccatt cgcgccaggg cgcaatcacc
     5101 ttgatgtgcg gctgcaaggc gtaatagctg agctcgaagc gcacctggtc gttgcctttg
     5161 ccggtcgccc catgcgcgac ggcatcggcg cccacttgac gtgcgatctc gatctggcgc
     5221 ttggcgatca gcggccgcgc gatcgacgtg ccgaggaggt attggccttc ataaagcgcg
     5281 ttagcgcgga acatcggaaa gacgaagtcg cgcacgaact cctcgcgcag atcctcgatg
     5341 aagatctgtt tgacgccgag cagctcggcc ttcttgcgcg ccggctcgag ctcctcgcct
     5401 tgcccgagat ccgcggtaaa ggtcacgacc tcgcattcat aggtctcttg cagccagcgc
     5461 aggatcaccg aggtgtcgag cccgcccgaa taagccagaa cgatcttttt taccgtgccg
     5521 cccatgacgc gatgacctct tgcgcgtgcg tgaaaaatgc gcgggcgagt atagggaagc
     5581 ggccggtcgc ggcaaagccg gacgcctgct cctccgttgt gcgccggcac cgcgtcgcgt
     5641 aaggtcgggc gccgatttcg gcgcgaagca gggagggaca cgcggtgaaa cgaacggtcc
     5701 ggtatgtgtc ggcggtaagc gcggtcatgt tgctgccgat attgggcggc caagcggcgc
     5761 aagcggcgga gatcctcacg ctgaaatcgc cggcgctcaa ggacaatggc gtgctcgcca
     5821 agaaatacgc gggaaacgac acgtcgaatc cgaactgcgt cggcgaaaac atctcgccgc
     5881 cgctcggctg gtcgcacccg cccgagggca cgaagagctt cgcgctcatc atgtatgatc
     5941 ccgaaggccg ctctggcctc ggcgtcgtgc attgggtcgc ctatggcatt cccgccgagg
     6001 tgaccggctt cgccgaaggc gaggtgagca aaccctcgga caagttcgtc ggcggcaaga
     6061 gcacgcgcgg gcttgcgacg tatttcgggc cctgcacgcc gcccggcgac tggcaccact
     6121 atacctttac gctcatcgcc accgacctcg accccaaggc gctccagccg ggcatgacgc
     6181 gcgacgagct gctggcggcg ctccagggac acggcaaggg cgcggcaggc ttgatcggcc
     6241 gcttcaagca taaataaacc cgcgcgacgc ctgcgcgacg cgaaagcgtc gtcgcgaccg
     6301 ggcgccagcg gttgctcttg caactgcgag agcccggatc ggtgcggctt tcgcgattgc
     6361 tgcgcaatcg ctgtcgccgc ccgccctaag gaacgcgcga cgcgcgttcc ttagggcgtg
     6421 acccgccgaa tgaaagccgc cgcctcggcg agggccgcgc gggcctcggg gagccgcggc
     6481 gcgaagatct gccagacatg cggcaggcgg tgccactgct tcagatcgac ggtgacgccg
     6541 gcggcggtcg ccttcgcggc gagccgcgtc gaatcatcga ggatcacctc gctgtcgctc
     6601 acctggacga agagcggcgg cagcgcgcgc agatcgccat agagcggcga ggccagggga
     6661 tgggtcgccg gcgcgtcgcc aagatagacg cgtgcgccct tggcgacgga atagccatga
     6721 aacatcacgt cgcggcggtc gttcgtcacg agcgatgcgc cggtcgcggc gagatccgtc
     6781 cagggcgaga acagcaccgc gccggccggc aaggtgtcgc cggcatcacg cagcgacagc
     6841 agcgcggcga gcgcaagccc gccgcccgcc gaatcgccgc cgacgacgat acggctgggc
     6901 ggcgtgccga gcgcgacgag gcggcgatag gccgccaccg catcctcgac cgccgccggg
     6961 aagcgatgct cgggcgcgag gcggtagacc ggcgcgacgg ctcgcgcatc ggcgccggtc
     7021 gcaagcgcga atgtgatcgg acggtgcgtt gccggagagc agaagcaata gccgccgccg
     7081 tgaaaataga ggagggtgcg cgccgcgggt gcgccgtccg caggctcgat ccagtcgccg
     7141 gccagcgccg cgtccgtgcc ctggcggacg cgccagcctt tcggcacgag cggatcgcgg
     7201 aaggtcttcg ccacgaattc gcgcgccctc gcgggatcgg gctcgcgttt gctgagcggc
     7261 ttcgtccgcc agcgcagcag ctgcgagaga acgacattct gccaactcat gaaaacaccc
     7321 cggctcgcct cgtcccttgc tcgatgctag catcgcgatt ccgggaaaga ggcgggagat
     7381 cctcatgatg acagccgcca cgacgatgcg ccaagtgtgg atcccgcggc acgggccgcc
     7441 cgaggtgctc gaggtccgcg acgctcccat ccccgagccg aagagcggcg aagtgctggt
     7501 caaggtcgcc gctgccggca tcaacttcgc cgacatcatg gcgcgcctcg gcctctaccc
     7561 cgatgcgccc aagccgccct gcgtcgtcgg ctacgaggtg gcgggcgagg tgatggccct
     7621 gggtccatcg gcgagcggcg ttcgcgccgg cgaccgcgtc atcgcgatga ccgcgttcgg
     7681 cggctattcg agccatgtga gcgtgcccca agcgcaggtc tttccgctgc ctgccggcat
     7741 gaccttggag cagggcgcgg cactccccgt gacttatctc acggcatatc agctcatgat
     7801 cgccatgggt cggctgagaa gcggcgagtc cgtgctcgtc cattccgcgg cgggcggtgt
     7861 cggtctctcg gcgatcgaga tcgcggccat catcggcgtc aaggtgatcg gcgtggcctc
     7921 tgcggcgaag catgagtatc tgcggagccg cggcgtaatg gggctgatcg agtccagttc
     7981 cagcgatctt ccgagccgcg tcaaggagct gacggccggt cggggcgtcg accttgcgct
     8041 cgacgcgcgc ggcggctcct cttggcggca aagctatgat tcgctcgccc cgaccggccg
     8101 gcttgcgatg ttcggcctgt ccacggcggt cggcggcaat ccctggctgg gcacggcgcg
     8161 ggcgcttctg ggcgtgccat ggctgcgctt caacccgctc accttgatga gcgacaatcg
     8221 cggcatcttc ggcgttaata tcggccatct ctggggccat caggaattgc tcggcgaatg
     8281 gatgagacag atcctggcct ggcaaagtca aggcaagatc gcgccggtcg tggcgcgaag
     8341 cttccgtttc gacgaggctg ccgccgccca tcgctacatc gagaaccgca gcaacgtcgg
     8401 caaggttctg ctggtgccct gaggccggga cgtcagggcg gtgcagcgtc gcttgggaag
     8461 agccggccga ggacattcca gtagcgcacc ggcaggaggc gctgcagccc gtcgagaaac
     8521 cgcgcatcgc cgccgatgag aatgcgcttc tcgttgcgcc ggatgccgcg caggatgcgc
     8581 tcggcggccg cgtcgggggt ggtgcgcgcc gcctgctcga accgcgtgat gacgtcgtcg
     8641 cgctgggcgt tgccgacgcc cgccgccgcg cgcgcatggg taacgatcgg cgtgcggatg
     8701 ccgcccgggt ggacggtgga gacgcggacg ttgctgccgg cgagctcgtg cgccagcgac
     8761 tcgctgaagc cgcgcacggc gaatttgctg gcgcaatagg cggcgttgcc ggacggagcg
     8821 atcagcccga agacgctcga aatgttgacg atatgcgcct cgggacgctg cttgagcgcc
     8881 ggcaggaaca gcttcgcgcc atggacgacg ccccagaagt tgatgtcgac cagccagcgg
     8941 atgtcgtcga ggctcacctc ctcgaccgtg cccaggagcg cgacaccggc attgttgatg
     9001 aggagatcga cgccgccatg ctcgccgagg acggtgtcgc ggaatgcggt catctggtcg
     9061 agcgcggaga cgtcgacgcg atgggcggtc gcgcgcgacc cggcctgccg cgccgccgcg
     9121 actgtttcgc cgaggccccc cgcgtcgata tccgcgagcg ccaattggca gcccgcgcgt
     9181 gccaggcgaa gcgccaggct gcggccgatg ccgcttgcgg cacccgtcac gacggcgacg
     9241 gccccttcga gcttctgcat aggacgcttt ctagccgacc ggacggcgcg cggcgaccgg
     9301 caagagggtc gccagcggcc atttttcggc accccacgtc gagtggctag gcaacgcctt
     9361 cggcatacgc taaggttttt gcaacgaatc tagggtcaac cccctagaag ttaaagggaa
     9421 atccgcggcg ggccagtgcg gcaatgaaga cgcgcgacag gatcttgaag acggcgctcg
     9481 cgctcttcaa cgagagcggt gaggccggcg tgtcgctggc gcagatcgcc gccgcgctcg
     9541 acatcagcga aggcaacctc tggtaccact tccgcaccaa gcgcgacttg atcgcggcgc
     9601 tcctcgacga gcttgagcag acgatcgagc agcaacacga gcgtcccccg gcagatcccg
     9661 gcaatgtcag tacttacgcc gactactcgc ggcaatgctt ccgcacgatg tgggcctatc
     9721 gcttcctcta tcgcgcccgc ttcgatccgg ggaaggaccg ggagctggcg gagcgccgcc
     9781 tcgccatcac ggtgcgcggc catcaccata gcgagcgcca tctcaccgag atggcgcgct
     9841 gcaagctgct cgatgcgagc ccgtccgaga ttgccgagct cgtcaccgcc gatcgcatcg
     9901 tcagtcgcta ctggttcgac tatctgcagg agcgctacgg cctggtgcag ctttccgagg
     9961 ccgaccttca gtcgggggtg aagcagattt ttgcgcttta ccggccgtat ctctcagcgg
    10021 cggcgcgagc gcaaatcgct gcggccatgc cacccgggcg caaggcggcg cgccgttagt
    10081 ctaggtccgc ggcgcgtgat cctgttccag ctcaggacgc gccgatcacc tcgcgcagct
    10141 tctcgatcag atcggccatc cggtagggct tgttcagaat tgggaaaggg ccgtggtcgc
    10201 cgagcgcggc ctcgcggccc acggccgcgt agccggtggt cagcaggatc ttcagcgagg
    10261 ggcgaagcgt gccggcttcg cgggcgagct cgacgccgct catgccgccc ggcatcacga
    10321 tgtcgctgaa catgagatcg acgggcttgc ccgacagcag gatgctcatc gcctcgcgcg
    10381 cggttccggc cacaagggtc cggtatccca gcgccgtgac cgacgacacc accatctcgc
    10441 gcacaggcgc attgtcctcg acgacgagaa tcgtcgcctg ggcgttgcgt gactccggca
    10501 tgctcccggc cgcgttgtcc atgtcgacga cgccttcggc gcgcggcaga tagatgcgca
    10561 ccgtggtgcc gacgcccggc gcgctctcga tcgcggcgtc gccttccgat tgccgggcga
    10621 agccatagac ctggctcaac ccgaggccgg tgcccttgcc gatctccttg gtggtaaaga
    10681 aggggtcgaa ggcgcggccg acgatctccg tggtcatgcc gatgccgttg tcgctgaccg
    10741 ccagcatgac atattggccg gacgcgagct ccggcatcgc tgcgtcgttt ctgatgcgca
    10801 cattgcgcgt ctcgatcacg atctggccgc cgtcgggcat ggcgtcgcgc gcgttgacca
    10861 cgaggttgag cagcgccgac tggaactgcg ccggatcgac cagcgccggc cacaagcgcc
    10921 cttcgggatt gaaggtgacg tcgatggtct cgccggccgc tcggttgacg atgatgcgga
    10981 actcctggat gagcgtgttg aggtcgacga tttgcgggcg cagcgtctgc tggcgcgcga
    11041 aggcgaggag ctgctgggtc agccgcccgc cgcgttgcgc cgcgcgctgc gccgcgtcga
    11101 tcaggcgatg caggctctga ttgttgccga tgccggcggc gacgaggtcg agattgccgg
    11161 cgatcgccgt cagcaggttg ttg
//
LOCUS       DASYNS010000318         4950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4756467, whole genome shotgun sequence.
ACCESSION   DASYNS010000318 DASYNS010000000
VERSION     DASYNS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4756467"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VGP50_09820"
     CDS             complement(<1..704)
                     /locus_tag="VGP50_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8027718.1"
                     /translation="MAERALRGISFRGGGFAPQPVKRAASIVFALILVVWQVAISSGL
                     VSAVFLPSPWSILLALRDLAVSGELWRDLSISLLRIAAGWALGTAAGLGVGLAMGLQS
                     VARSIGVPLVSALFPIPKIALLPLLILWLGIGEAPKIATIALGVFFPTAIAAASGIDS
                     VPRGLIRMAQSFNMPFAAILAKVVLPGALPSIIAGFRISASTALLLVVAAEMIGAQDG
                     IGAFVLAAGNLMQTDRL"
     gene            complement(697..1470)
                     /locus_tag="VGP50_09825"
     CDS             complement(697..1470)
                     /locus_tag="VGP50_09825"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027719.1"
                     /translation="MELVAEGVAHAYGSLEVLSGIGLRVAPGEIVVVIGPSGCGKSTL
                     LAILGGLIAPDSGAVLTEGAPPADCLNALTFVFQDSALLPWRTVAGNVALPLEHHGLV
                     AAERSERVAAALARLGLAEFRDAFPRQLSGGMRQRVGIARALVVRPAVLLMDEPLSAL
                     DAQTRELVMEDFLGLWLDQRVASVYVTHNLDEALRLADRIVVLSRRPGRVKTVMPIAI
                     PQRERRAAAARGELARLGDTLWQLLRDEAVEADREVVHG"
     gene            complement(1473..2471)
                     /locus_tag="VGP50_09830"
     CDS             complement(1473..2471)
                     /locus_tag="VGP50_09830"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027720.1"
                     /translation="MIRRGWAGLLALALLALAVPAEAAEKLRVGVLRLASSGPVFIAS
                     ERGYFTAEGVEAELRFFDAAQPIAVAAVAGDIDVGVTGLTAGFYNLAGKGALRIIGAQ
                     SREEPGYHLVAYLASNQAYESGLKSLKELPGHSVAITQVGSTFHYSLGLLAEKLGFAL
                     SQVRLVPLQSMGSMSAALESGQVDAALIPATLALPMTANGKAKMLGWVGDETPWQLGA
                     IFTGARTIAERRGALTHFIAAYRHGARDFHDAFLAHKVDGAEAADLLAIIAKYTAQSP
                     EQLRPGIPYVEPDAKLQVPDIYRQVAWYQGQGLVDAAVDARAILDLSFTDPSPEPR"
     gene            2614..3039
                     /locus_tag="VGP50_09835"
     CDS             2614..3039
                     /locus_tag="VGP50_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027721.1"
                     /translation="MTAEKNLDNLSGFWTGVYDYPVTHREPVPFNAVIEDVGGALSGE
                     IVEPNTFSQAKERELFANISGTRQGTAVHFIKTYERVPRGGHSLVYQGTLDGTGTRID
                     GTWRAHAGWSGPFVMNRSSGKKAKIEDTIERALDLVKSR"
     gene            complement(3177..4211)
                     /locus_tag="VGP50_09840"
     CDS             complement(3177..4211)
                     /locus_tag="VGP50_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV8027722.1"
                     /translation="MSRSALSRGEPPVLVEVVRGEWVESGHRGAVAVVDAAGKVARSL
                     GDIDRLVYPRSALKPIQALPLLETGAADGFALGDEELALACGSHRGELMHVEKVAAWL
                     ARIGLSAADLECGAHPPRDEPSYQALLRRGDPASPLHNNCSGKHAGFLTTARWLKEPT
                     RGYIGADHPVQQRVARIAGEMTGLDLARAPRGRDGCGIPVIAMPLRALATAMARLADP
                     SALGEHRRAAVQRILAAMAAAPLLVDGTGGMTTRVMTIASATVRLKPGAEGVFCAALP
                     ALGLGVAIKIDDGAGRAADLAMAAVLDRLGCFTAEQRAALEPFLVPVIKTVAGKDAGR
                     LQPGAALQSF"
     gene            complement(4256..4867)
                     /locus_tag="VGP50_09845"
     CDS             complement(4256..4867)
                     /locus_tag="VGP50_09845"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEV8027723.1"
                     /translation="MDVQRLSVEYGAWFYLLTFAWTFIEGETFVLVAGFAAAQGLLNA
                     PLLLLAAWLGSFAGDQFYFWIGRNFGLRLLSRRPVWRNRVDSALGWVKRYDKWFILSF
                     RFIYGIRNFSSFALGISGIAWQRFLVLNLTAALLWATTFVGAGFFCGRTFDRALDRML
                     GERADRFSLLMLVAFALVLVAGGLVYRFRRRRRVAPAATQTSV"
BASE COUNT          932 a         1703 c         1681 g          634 t
ORIGIN      
        1 agccgatccg tctgcatgag attgccggcg gccaggacga aagcgccgat gccgtcctga
       61 gcgccgatca tctctgccgc caccaccagc agcagcgccg tcgaggcgga gatgcggaag
      121 ccggcgatga tgctcggcaa cgcgccgggc aagacgacct tggcgaggat cgcggcgaac
      181 ggcatgttga agctctgcgc catgcggatg agaccccgcg gcacgctatc gatgccgctc
      241 gccgcggcga tcgccgtcgg aaagaacacg cccagcgcga tcgtcgcgat cttcggcgcc
      301 tcgccgatgc cgagccagag aatcagcagc ggcagcagcg cgatcttggg aatcggaaag
      361 agcgcagaga ccagcggcac gccgatcgag cgcgcgaccg actgcagtcc catggcgagg
      421 ccgacgccaa gtcctgcggc cgtgcccaag gcccagccgg cggcgatgcg cagcagcgaa
      481 atcgacagat cccgccagag ctcgcccgaa accgcaagat cgcgcaacgc caggagaatg
      541 ctccacgggc tcggcaggaa gaccgcactc acgagaccgc tcgagatcgc cacctgccag
      601 acgacgagga tcagcgcgaa gacgatcgac gcggcgcgct tcaccggctg cggcgcaaag
      661 ccgccgccgc ggaaggagat gccgcgcaac gcgcgctcag ccatggacga cctcgcggtc
      721 ggcttcgacg gcttcgtcgc gcaggagctg ccacagcgtg tcgccgaggc gcgcaagctc
      781 gccacgcgcc gcggcggcgc ggcgctcgcg ctgcggaatg gcgatcggca tcaccgtctt
      841 cacgcgaccc ggccgccgcg acagcacgac gatgcgatcg gcaaggcgca gcgcctcgtc
      901 gagattatgc gtgacataga ccgaagcgac acgctgatcg agccagagcc ccaggaagtc
      961 ctccatgacg agttcgcgcg tctgcgcatc gagcgccgac agcggctcgt ccatcagcag
     1021 cactgccggg cgcacgacca gcgcgcgggc gatgccgacg cgctggcgca tgccgcccga
     1081 gagctgccgc ggaaaagcgt cgcggaactc ggcgaggccg agccgcgcga gcgctgcggc
     1141 gacgcgctcg ctgcgctctg cggcgacgag cccgtgatgc tcgagcggca gcgccacatt
     1201 gccggcaacc gtgcgccagg gcagcagcgc cgaatcctgg aagacgaaag tgagcgcgtt
     1261 gagacagtcg gccggcggcg cgccttcggt cagcacggcg ccgctgtcgg gggcaatgag
     1321 cccgcccagg atggcgagaa gggtcgattt gccgcagccc gagggcccga tgacgacgac
     1381 gatctcgccg ggcgcgacgc gcagtccgat gccgctcagc acctcgagcg aaccataggc
     1441 atgagcgacc ccttccgcga cgagttccat cgctaacgcg gctcgggcga cgggtcagta
     1501 aaggaaagat cgagaatcgc acgcgcgtcg accgctgcat ccaccagccc ttgcccctgg
     1561 taccaggcga cctggcggta gatgtccggc acctggagct tcgcatcggg ctcgacatag
     1621 gggatgccgg gtcgcagctg ctcgggggat tgcgccgtgt acttggcgat gatggccagg
     1681 agatcggcgg cttccgcacc gtcgaccttg tgcgcgagga aggcgtcgtg gaaatcgcgg
     1741 gcaccgtggc gataggcggc gatgaaatgc gtgagcgcgc cgcgcctttc ggcgatcgtg
     1801 cgtgcgccgg tgaagatcgc gcccagctgc cacggcgtct cgtcgccgac ccagcccagc
     1861 atcttcgcct tgccgttcgc cgtcatcggc agcgccagcg tcgccgggat cagcgccgca
     1921 tcgacctggc cgctctcgag cgcggccgac atgctgccca tcgactggag cggcaccagc
     1981 ctcacctgtg acaaggcaaa accgagcttt tcggcgagaa ggccgaggct gtagtggaac
     2041 gtcgacccga cctgggtgat cgccaccgaa tgccccggca gctccttgag gctcttgagc
     2101 cccgactcat aggcctggtt cgaggcgaga taggcgacga gatgatagcc cggctcctcg
     2161 cggctctgcg cgccgatgat gcgcagcgcg cctttgccag cgagattgta gaagccggcg
     2221 gtgaggccgg tgacgccgac atcgatgtcg ccggcgaccg cggccacggc gatcggctgc
     2281 gccgcgtcga agaagcgcag ctccgcctcg acgccctccg cggtgaaata gccccgctcg
     2341 gaggcgatga agaccggacc cgaggaggcc agccggagca cgccgacgcg cagcttttcc
     2401 gcagcctcgg ccgggacggc cagcgccagc agggccaagg ccaggagacc cgcccaccct
     2461 cgtcgaatca tgtcggttcc tcccctcggc gcgggaggat gcggctccgg tggcacgttg
     2521 gcaaccctgg cgctgccgat atccggcggc ctatagtgcg cgcaagcgcc gcactagccg
     2581 gctccgcctc ttcaagcaaa ttgctccgcc gccgtgaccg ccgaaaagaa cttggacaac
     2641 ctctccggct tctggaccgg cgtttatgac tatcccgtca cgcatcgcga gccggtgccc
     2701 ttcaatgccg tgatcgagga tgtcggcggc gccctgagcg gcgagatcgt cgagcccaac
     2761 accttcagcc aggccaagga gcgagagctg ttcgccaaca tctccggcac ccggcaaggc
     2821 accgccgtgc atttcatcaa gacctatgag cgcgtgccgc ggggcggcca ttcgctggtc
     2881 taccagggaa cgctcgatgg caccggcacg cggatcgacg gaacatggcg cgcccatgcc
     2941 ggatggtcgg gcccgttcgt gatgaaccgc tcgagcggca agaaggccaa gatcgaagac
     3001 accatcgagc gcgcgctcga cctcgttaaa tcgcgctgac aacccctttc caactcagga
     3061 gcacccatga gccgatccgc caagtccagc gtccagattc gtcagaaccg gcgcaaggca
     3121 aagcgcaaag cgaaacggcg gcggcagcgc gcgcgggcga cgaagtagcg gccgcttcag
     3181 aaggactgaa gcgcagcgcc gggctgtagc cgcccggcat ccttgccggc aacggtcttg
     3241 atcaccggca cgaggaacgg ctcgagcgcc gcgcgttgtt ccgcggtgaa gcagccgagc
     3301 cggtcgagca cggcggccat ggcgagatcc gccgcgcgcc cggcgccgtc atcgatcttg
     3361 atggcgacgc cgagaccgag cgcgggcagc gccgcgcaga aaacgccctc ggcgccgggc
     3421 ttgagccgaa ccgtggcgct cgcgatcgtc atgacgcgcg tcgtcatgcc gccggtcccg
     3481 tccaccagga gcggcgccgc agccatcgca gcaaggatcc tctggaccgc ggcgcggcga
     3541 tgctcgccga gcgccgaagg atcggcaagc cgcgccatcg ccgtcgccaa cgcacggagc
     3601 ggcatggcga tcaccgggat gccgcagccg tcgcgcccgc gcggcgcccg cgcaaggtcg
     3661 aggccggtca tctcgccggc tatgcgcgcg acgcgttgct gcaccgggtg gtccgccccg
     3721 atatagccgc gcgtcggctc tttgagccag cgtgccgtcg tcagaaagcc ggcatgcttt
     3781 ccggagcaat tgttgtggag cggcgaagcc ggatcgccgc ggcgcagcag cgcctgatag
     3841 gagggctcat cccggggtgg atgcgcgccg cattcgagat ccgccgcgct gaggccgatc
     3901 cgcgcaagcc acgccgccac tttctccaca tgcatgagct cgccgcgatg agagccgcag
     3961 gccagggcca attcctcgtc gccgagagcg aatccgtcgg ccgcgcccgt ctccagcagc
     4021 ggcagcgcct ggatcggctt cagcgccgag cggggataga cgagccggtc gatatcaccc
     4081 agcgagcgcg ccaccttgcc ggcggcatcg acgacggcga cggcgccgcg atggccgctc
     4141 tcgacccatt cgccgcgaac cacttcgacg agaaccggcg gttcgccccg agaaagcgct
     4201 gatcgcgaca tgggcagcgg ccctccgcgg ccgcgaaaga gcgaaaagcc gacggctaga
     4261 cggaggtttg cgtcgcggcc ggggccactc tgcgccggcg ccggaaacga tagacgagac
     4321 cgccggcgac cagcacgagc gcaaaggcga cgagcatcag caggctgaag cggtcggcgc
     4381 gctcgcccag catgcgatcc aaagcgcgat cgaacgtgcg gccacagaaa aagccggcgc
     4441 cgacgaaggt cgtggcccag agcagcgccg ccgtcaggtt gagaaccagg aaccgttgcc
     4501 aggcgatgcc gctgatgccg agggcaaagg aagagaaatt gcgaatgccg tagatgaagc
     4561 ggaagctcag gatgaaccac ttgtcatagc gcttcaccca accgagcgcc gaatcgaccc
     4621 ggttgcgcca gacgggccgg cgcgacagga ggcgcaggcc gaaattgcgg ccgatccaga
     4681 aatagaactg gtcgccggcg aagctcccca gccaggcggc aagcaacagc agcggcgcgt
     4741 tcaggagacc ttgcgcggcg gcaaagccgg cgacgagaac gaaggtctcg ccctcgatga
     4801 aggtccaagc gaaggtcagg agatagaacc acgcgccata ttctacgctg aggcgttgca
     4861 catccatctt cttgtttcct ccccccggag gggcagacga tcctacgcgc tcggagttac
     4921 gaagtcgaga atcggccaac ccgctatggg
//
LOCUS       DASYNS010000319         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1013364, whole genome shotgun sequence.
ACCESSION   DASYNS010000319 DASYNS010000000
VERSION     DASYNS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013364"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VGP50_09850"
     CDS             <1..427
                     /locus_tag="VGP50_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8027724.1"
                     /translation="RGSDLGSFIGDATKLRQVVLNLVANAAKFTENGVITLSVARKRQ
                     ADGDWLAIAVRDTGIGISPENLEKLFTNYTQADPLIGQKYGGTGLGLALSQRFCRLMG
                     GEIVSESIIDRGSCFTIRIPVAMTGATPALERRPVEQVR"
     gene            496..2217
                     /locus_tag="VGP50_09855"
     CDS             496..2217
                     /locus_tag="VGP50_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEV8027725.1"
                     /translation="MAGLSISEATAALTAPGAPFAMAEKEIGGIKLRVWRNAPPSLRA
                     VLEASRGHGALPFLVYEDERLSYVEHYRRSAALAHAMIERFGIRKGERVAIAMRNFPE
                     WSIAFWATVAIGAIAVPLNAWMTGAELEYCLADSEAALLIADGERAERLLPHLPRLPL
                     RATLVARPGAPPRPPLLDLEEVAAAAGDVTLPEATIDPDDDATIFYTSGTTGKPKGAL
                     GTHRNVCTTITSASFGRARALLRRGEPLPEPGTAPKRSSLLSVPFFHATGCHSVLCPT
                     VHAGNKIVLMRRWEPERAMQLIERERITAFGGVPAMAWQVLEHPAFDRYDLSSIEGVS
                     YGGAPAASELVARILQKFPKVQPSQGYGLTETSAVLTGISGEDYRQKPDSVGPSIPVC
                     DVRVMDDAGHDVAVGAAGELWVQGPNVVKGYWRNPEATAAGFGGGWLRTGDLARIDAE
                     GFVYVLDRLKDMLIRGGENIYCVEVEDALYSHPAVMDAAVVGIPHRVLGEEVGAVVQL
                     KPGASATEEDLKRHVAARLAAFKVPVRIVLEREPLPRNASGKIMKRELRGVVGGQSST
                     VSRGSER"
     gene            2247..>2863
                     /locus_tag="VGP50_09860"
     CDS             2247..>2863
                     /locus_tag="VGP50_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027726.1"
                     /translation="METTRVRPRNAATLILLRDGARGLEVLIGRRGAGARFMPGRYVF
                     PGGRVMSSDALPWCGEPGGTEARALLALKRAALRETFEETGLVVGRPADATADGPAGS
                     AIEHAYAARHLAPAPDLLRLVGRAITPAASPIRFHARFFLADGRHAAGELAPSEELDH
                     VYWHALEEPPPMPMQSVTRFMLTCAIDAWKGQPAAEPPLYWHRGNR"
BASE COUNT          442 a         1024 c          969 g          428 t
ORIGIN      
        1 gcgcggcagc gatctcggct ccttcatcgg cgacgccacc aagctgcgcc aggtcgtgct
       61 gaacctcgtg gccaacgccg cgaaattcac cgagaacggc gtcatcacgc tgagcgtcgc
      121 gcggaagcgc caagccgacg gcgactggct cgccatcgcg gtgcgcgaca ccggcatcgg
      181 catcagcccc gagaacctgg agaaactttt caccaactac acccaggccg acccgctcat
      241 cggccagaaa tatggcggga ccggcctcgg cctcgccttg agccagcggt tctgccggct
      301 catgggcggc gagatcgtct ccgagagcat catcgaccgc ggctcctgct tcaccatccg
      361 cattcccgtc gccatgaccg gtgccacgcc ggcactcgag cgccgtcccg tcgagcaggt
      421 aagatagccg gctagcgaag tcgccagtta tcagttatca gtcgtcggtc gggcgcaacg
      481 gacaactgac aactgatggc tggcttatcc atctccgaag cgaccgcggc gctgacggcg
      541 ccgggcgctc ccttcgcaat ggcagagaaa gagatcggcg gcatcaagct gcgggtgtgg
      601 cggaacgcgc cgccatcgct gcgcgccgtg ctcgaggcga gccgcggcca tggcgcgctc
      661 cccttcctcg tctatgagga cgagcggctc tcctatgtcg agcattaccg ccgctctgcc
      721 gctctcgccc atgcgatgat cgagcgcttc ggcatcagga agggcgagcg ggtcgccatt
      781 gccatgcgca actttcccga atggtcgatc gccttctggg ccaccgtggc catcggcgcg
      841 atcgccgtgc cgctcaatgc ctggatgacc ggggccgaac tcgaatactg cctcgccgat
      901 tctgaggccg cgttgctcat cgccgatggc gagcgcgccg agcggctgct gccgcatctg
      961 ccgcgcctgc cgcttcgcgc cacgctggtg gcgcgccccg gcgcgccgcc gcggccgccg
     1021 ctcctcgacc tggaggaggt cgccgccgcg gccggcgacg tcacgctccc ggaggccacc
     1081 atcgaccctg acgacgatgc gacgatcttc tacacctcgg gcacgacggg aaagcccaaa
     1141 ggcgcactgg gtacacatcg taacgtctgc acgaccatca ccagcgccag ctttgggcgc
     1201 gcgcgtgcgc tgctgcggcg cggcgagccc ttgcccgagc ccggcacggc gcccaagcgc
     1261 agctctctgc tctcggtgcc cttcttccac gcgacgggtt gccactcggt cttgtgcccg
     1321 acggtccatg ccggcaacaa gatcgtgctc atgcgccgct gggagcccga gcgggcgatg
     1381 cagctcatcg agcgcgagcg cataaccgca ttcggcggcg tgcccgcgat ggcgtggcaa
     1441 gtgctcgagc accccgcctt cgaccgctac gacctgtcga gcatcgaggg ggtctcctat
     1501 ggcggcgcgc ccgcggcctc cgagctggtg gcacgcatcc tccagaagtt tccgaaggta
     1561 cagccgagcc agggctacgg cctcaccgag acctcggccg tcctcaccgg catctcgggc
     1621 gaagactacc ggcagaagcc cgacagcgtc ggccccagca tccccgtctg cgacgtccgc
     1681 gtcatggacg acgccggcca tgacgtggcg gtgggcgccg cgggcgagct ctgggtgcaa
     1741 gggccgaacg tggtcaaagg ctactggcgc aatcccgagg cgaccgcggc ggggttcggc
     1801 ggcggctggc tgcgcacggg cgatctcgcg cgcatcgacg ccgaaggctt cgtctacgtg
     1861 ctcgaccggc tcaaggacat gctgatccgc ggcggcgaga acatctactg cgtcgaggtc
     1921 gaggatgcgc tctacagcca ccccgctgtg atggacgcgg cggtggtggg aatcccccat
     1981 cgcgtgctgg gggaggaagt cggagcggtc gtgcagctga agcccggcgc gtcggcgacc
     2041 gaggaggatc tgaagcgcca cgtcgcggcg cggctcgccg ctttcaaggt gccggtccgg
     2101 atcgtgctcg agcgcgagcc gttgccgcgc aacgccagcg gcaagatcat gaagcgggag
     2161 ctgagaggag ttgtcggtgg tcaatcctcg actgtcagcc gcgggagcga acgctgatcc
     2221 tgggacccga gaagcgggat gcgaagatgg aaacgacccg cgtccggccg cgcaacgcgg
     2281 cgactctgat cctgctgcgc gacggcgcgc gcgggctcga ggtgctgatc ggccggcgtg
     2341 gcgcgggcgc gcgcttcatg cccgggcgct acgtcttccc cggcggccgc gtcatgtcga
     2401 gcgacgcgct tccctggtgc ggcgagcccg gcgggaccga ggcccgcgct ctcctggcgc
     2461 tcaaacgcgc ggcgctgcgc gagaccttcg aagagaccgg gctcgtcgtc ggacggccgg
     2521 cggacgccac ggccgatggg cccgctggct ccgccatcga gcatgcctat gcggcgcgcc
     2581 acctggcgcc ggcgcccgat ctcctgcgcc tcgtcggccg cgccatcacg ccggcagcct
     2641 cgccgatccg cttccacgcg cgcttctttc tcgcggacgg gcgccatgcc gcgggcgagc
     2701 tcgcgccgtc ggaagagcta gaccatgtct attggcatgc gctcgaggaa ccgccgccaa
     2761 tgccgatgca aagcgtcacg cgcttcatgc tgacctgcgc gatcgacgcc tggaagggtc
     2821 aacccgcggc cgagccgccg ctctattggc accgcggcaa ccg
//
LOCUS       DASYNS010000320        18207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4290335, whole genome shotgun sequence.
ACCESSION   DASYNS010000320 DASYNS010000000
VERSION     DASYNS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 18207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18207
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4290335"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VGP50_09865"
     CDS             <1..1207
                     /locus_tag="VGP50_09865"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEV8027727.1"
                     /translation="RVAIADTAGAAWAVARCGSAPAAVVPEGGVRAVLAPLPVLALRL
                     DPALAGELERLGLRRIGDLYALPRAALVTRFGETATLRLDQALGVAPEPLSPLPPAPA
                     RWTRRRFAEPIGTAEAIAAATRVLLDALCRRLADEGLGLRRLALRLYRVDGSSAEAAI
                     GTARPSRDVRHLLRLLEEKLPAIDPGLGIEDMLLEAARVERLAAAQLSFRNALHHFPV
                     VPAKAGTQGERRAVALGPRFRGGDDGEKAKGSVDETEVAALVDRLANRLGPGTVFAIL
                     PRESHVPERAVRRAPVFTTTKAAAWDPQRLRPIRLLPRPEPIEAVAPIPDDPPILFRW
                     RRLQHQIRRADGPERILGEWWRSAAEAEELRDYYRVEDEAGRRFWLYRLGLYRPEAPP
                     RWFLHGLFA"
     gene            1191..4592
                     /locus_tag="VGP50_09870"
     CDS             1191..4592
                     /locus_tag="VGP50_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEV8027728.1"
                     /translation="MDSSPEVMAQYAELQVTSNFSFLRGASHPHELVAEAKAIGHGAI
                     AIADRNTLGGVVRAHAAAKELGFRLVIGCRLDLLDAPSLLCFPTDRAAYGRLARLLTL
                     GKRRAEKGGCRLAYGDVVAHGAGQVVVAVAPEDAPDFADFLKRVAGDFPDAAYLAAQH
                     LYGGDDGRRVHRLAALAERARLPLLATNDVLYHRRERRALQDVMTCIREGCTIAEAGL
                     RLNAHAERHLKPAAEMARLFASYPDAVARSLEIVERCCFSLDELRYEYPDEAGNDGTP
                     AQPRLERLTWEGARRRYPQGVPEKIRKQAETELRLIGELGYASYFLTVQDIVAFARGE
                     GILCQGRGSAANSTVCYCLGVTAVDPEQVDLLFERFVSTERKEPPDIDVDFEHERREE
                     VIQYIYRKYTRDRAGLTAVVSSYRSRGAIREVGKALGLSEDVTAALAGTVWGWSDDDV
                     HEEQVKSVGLDLADPTLRRALELAGELIGFPRHLSQHVGGFVITRGRLDELVPIENAA
                     MEERTVIEWDKDDLNVLGILKVDVLGLGMLSCLRRGFALLKQHKGEDLSLATIPKERP
                     EVYKMLGHADSIGVFQVESRAQMSMLPRLQPKNLFDLVVEVAIVRPGPIQGNMVHPYL
                     RRRTGQDPDTCPPALEEVLKPTHGVPLFQEQAMRIAMIAAGFSAVEADGLRRAMATFR
                     RNGDIHRFEEKFIKGMLDNNYERDFAERCFQQIKGFSDYGFPLSHAASFALLVYASAW
                     MKCFHPEVFAAALLNSQPMGFYAPAQILRDAETHGVVLQPVDVNHSDWDCTLARREAS
                     SRAGDSGCSCNRESPHRGCECTEPHCSNSLPLSPTAWGRGQGEGVSQPTRSQPAHVET
                     PLTLPSPPARAGGEGEIGGKARVGARPTHALRLGSRLVKGLAKEEAEKISEKRGSGYR
                     SVAELQYRAKLHRGALERLAEADALQSLGLDRRRALWAVKGLRDEQELPLFTPLAGVG
                     VKEAAAVLPPMGLGEEVVEDYAALGLSLKCHPVALLRDALDARRILRNGCLEVTPAEK
                     RVRVAGLVLVRQQPGTASGIIFMTLEDETGIANIVVWRRVFERYRRVVMGAKLVECQG
                     RVQREGEVIHVVAERLIDLSPLLRQLWDPEARERPPLRLHSRDFR"
     gene            4663..5610
                     /locus_tag="VGP50_09875"
     CDS             4663..5610
                     /locus_tag="VGP50_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV8027729.1"
                     /translation="MPVIKVTDIAHGRLRAPDLDVMEEFLTHFGMVRAERTPAALYMR
                     GTGPARHIHVTEKGEPAFIGLAWHAADEGDLRRLATLPGASAVEQVDEPGGGKRVRLR
                     EPNGYQIDVLAGVTPLAPLAVARQAVNTGAETLKRKGELYRLRAGPSQVKRIGHGVMC
                     TPRLAETVRWFRETLGLIASDDLYAGSKDNLIGAFSRCDRGDEYVDHHVFFCLKHAKA
                     GLNHLSFEVQDMDDVFAGHHYLKRVGEYEHMWGIGRHLLGSNVYDYWADPWGRIHEHW
                     SDSDRLNARSGANLMPAEEGLVSQWGEPPPQKLLDRVSA"
     gene            complement(5734..6198)
                     /locus_tag="VGP50_09880"
     CDS             complement(5734..6198)
                     /locus_tag="VGP50_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027730.1"
                     /translation="MSKYRFASRILFAAVVALLPLAARADLPGEHPYYLHALSDLRTA
                     RWMLEHRPGDAAVSGQEDVAVQQIDAALGEIKRASIDDGKDLKDHPAVDVPKDHPGRL
                     HKAAELLRKVHQDVDREEDDPTAQGLKHRSLEHVEAALHATDQAIKDAETHK"
     gene            complement(6419..7558)
                     /gene="ugpC"
                     /locus_tag="VGP50_09885"
     CDS             complement(6419..7558)
                     /gene="ugpC"
                     /locus_tag="VGP50_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV8027731.1"
                     /translation="MAQVVLRSVAKKFDQTEVVRGIDLDIADEEFVVLVGPSGCGKST
                     TLRMIAGLEETSAGEILIGGQVVNDLPPKDRDIAMVFQNYALYPHMTVFENMSFGLRL
                     KKFPKHEIRTRVEHAARILDIIDLLARRPRQLSGGQRQRVAMGRAIVRNPKVFLFDEP
                     LSNLDAKLRVQMRTEIKKVHQKVRTTTIYVTHDQVEAMTLADRVVVMNGGRIEQIGTP
                     HDLYHRPQTRFVAGFIGSPAMNFLSCRLVEAAGGLSVRLADDLSLPVPAERTAAYRQH
                     LERPLVLGLRPEHVTETRSYGTGAEFAMPLDVVEPMGMETMVYFLVDGVEVCARVSPE
                     AAAAPGERMRFMADMRHMHLIDPATDRVIAAPAAASATGSAKALA"
     gene            complement(7563..8453)
                     /locus_tag="VGP50_09890"
     CDS             complement(7563..8453)
                     /locus_tag="VGP50_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV8027732.1"
                     /translation="MAALAADTPRRRSSGSLRRNRRWALVASYFFLVLFAIFFLIPPY
                     YQLVTATKSSAQISNQTTDPWLPAIPVALDNIATLWNKTDFPIYLENTAIVTVCVVII
                     TMLIAVPAAFALGRMSFWGSGVLATGVFLTYLVPDSLLFLPLFKIVGGLGLLDSYWAL
                     VLVYPTLTVPFCTWIMIGYFQSIPKELDEAALIDGANHLQMLFKIFIPVALPGLIAAT
                     IFAFTVCWAAFVYPMAFIYSSDQLVLTAGTVTTLVKGDVYQWGMIMTAALIAAAPPVV
                     IYAFLMDYYIAGLTAGATKG"
     gene            complement(8453..9385)
                     /locus_tag="VGP50_09895"
     CDS             complement(8453..9385)
                     /locus_tag="VGP50_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV8027733.1"
                     /translation="MTDVLSRRLPIPRAEARPGGLRRFARRRSTIAFLFAVPLLAVVL
                     GLIVYPGLYAIYLSLLNKTQTRFVGLANYRFLFGRETFWMVVEQSAIFAGTAVLFKAL
                     IGIVTAHLVNNLPGKGQRKWRGMLLVPWVIPLSLSTLGWLWLLDPTYSAFNWFFAQIG
                     APQIPWLSAPAWARLSTILVNVWYGSPFFLIMYLAALKSVPEQLYEAASIDGANAWQK
                     FIHITLPMMRNIIAITVLFSLIVTFANYDIVRVLTGGGPRDSTHVFATWAFQLGIASG
                     DIPLGAAVSLFMFPILGISAIFILRGVRRRGREI"
     gene            complement(9608..10954)
                     /locus_tag="VGP50_09900"
     CDS             complement(9608..10954)
                     /locus_tag="VGP50_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027734.1"
                     /translation="MKTALTRRRFLKRAVLATTAAIAAPYMRSSYSAGRLTLGCWDHW
                     VPGANEVLAAICNEWGEKNHVEVHIDFITSQGDKDIMTASAEAQARTGHDVIAHRSWQ
                     VQVHRTVLEPMNDVVDRLVKEHGPIAPISEYLARDEDSWKAVPAIAGSQVKPCCSRID
                     LYKQHCGLDVTKVFPFDDKRDPALIDSWNWDLYLATAEKLFKAGYPVGLPMGQTSDSI
                     DWVGALFRSFGAVFVDDKDNIRVDSPETRAVLEYARKLMAQNPPEVYAWDDAGNNKWL
                     ISGKGASIMNPPSAWAVAKRDNPKVAEQCWTHDIPRGANGRFAPYLPWFYGAWAFGQN
                     KSAAKELIHHIVDKPQAKRQVAASSGYDLPSFKSYYDFDTWRTVEPPLGTVFNYPPRG
                     DEELSVAGYPARPDVAAQLYNQALQPVMIARVAQGGEKIDDVVKWAARECEGYLRS"
     gene            complement(11058..11762)
                     /locus_tag="VGP50_09905"
     CDS             complement(11058..11762)
                     /locus_tag="VGP50_09905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEV8027735.1"
                     /translation="MADVEALKSTIEVCMFDQYGTVVDIQTGLTQAVTPFLEAKGWAG
                     NPSSLVTWWRRTHFENSMIDALLHKEHTPYREIGQRALAYVLDRAGIPYRMEDVRALV
                     AEIERLRPFPEVPDALARLRRRYRLAVLSNGDPDMLETAKPHHGLAFDKLISVAVANS
                     FKPHVATYTKAAEIIGVERSRVLFVANHAFDCIGAKAAGMRTAFIDRRRRPFGATPHQ
                     PDLLVPTLRELADLMA"
     gene            11843..14995
                     /gene="putA"
                     /locus_tag="VGP50_09910"
     CDS             11843..14995
                     /gene="putA"
                     /locus_tag="VGP50_09910"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEV8027736.1"
                     /translation="MAFVLPAPLPAPDPLRRTLRAVYRADETETVERIVAAATLPEEI
                     NARISARARDLVAEARRRRLGKGGIDALLHEYALSTPEGVALMCLAEALLRIPDAATV
                     DRLIRDKLAPADWARHLGHSDSLLVNASTWGLMLTGRLLGSNADDGDLAAALRRLLAR
                     SGESVVRQAVTAAMRVLARQFVMGRTIAEALERAEAPEREGYRHSYDMLGEAARTAAD
                     ARRYFESYDAAIAAIGATVAGRDIAAAPGISVKLSALHPRYEEAQRARVLRELTPVLT
                     ALALRAKKAGIGFTIDAEEAERLELSLDLIAALALDPALAGWNGLGLAVQAYQKRALP
                     LLDWLADLARRSRRRLMLRLVKGAYWDSEIKRSQERGLGGYPVFTRKMSTDVSYLACA
                     RHILAAPDAFFAQFATHNAHTLAAVIELAGERSDYEFQRLHGMGEALYEQVVGAEKLG
                     RPCRVYAPVGSHEDLLAYLVRRLLENGANTSFVNRLVDEQAPTEEIVADPVARVAALK
                     EKPHPRIPLPRDLYGPERRNARGIDLSNPARQAALAEAMEAAAGPWQAAPLIGGTERP
                     GPARAALDPADRRREIGTVSETTPEHIETALAQAAAAAPGWDAVPAPARAAALERAAD
                     LLETRLADFMALIVREGGRTIPDALAELREAVDYCRYYALCARADFAAPIVLPGPTGE
                     RNTLALAGRGVFACIAPWNFPLAIFMSQVVAALAAGNAVIAKPAEQTPLVAAAAVRLL
                     HEAGIPGDVLHLLPGDGASVGATLVRDPRVAGIAFTGSTETARAINRALAERPGPIIP
                     FIAETGGQNALIVDSSALPEQVVGDAVASAFNSAGQRCSALRVLFLQDDIAPRVLAML
                     EGAMRELAIGDPGLLSTDVGPVIDDAARSALLAHAARMSRDGRLIAELPLPAGTEHGF
                     FFAPRAFEIDRLDLLEREVFGPILHVIRWRADALDPVLDAIAKTGYGLTLGIHSRIDD
                     TVRRIHQRLRVGNTYVNRNMIGAVVGVQPFGGEGLSGTGPKAGGPHYLHRFATERTLS
                     IDTTASGGNATLLSLQEEAG"
     gene            15037..16716
                     /locus_tag="VGP50_09915"
     CDS             15037..16716
                     /locus_tag="VGP50_09915"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV8027737.1"
                     /translation="MHDLVIKNALVFDGSKAPGRHVAVAVAQGRIAAVDADVGAGRRV
                     IDADGLALAPGIIDTHTHYDAQITWDAGVHPSPELGVSTVVIGNCGFTIAPCRAEHRD
                     LTLRNLTHVEGMSLAVLRSGIRWEFETFPQYLDVIERSGSVPNVAAFIGHSSLRVWVM
                     GRAATERAASAAEIDAMRRLLCEAVDRGAVGFATSTAPQHNGEGGTPMPSRLADESEL
                     RALVGVLGDAGRGVFMLTKGMSTPVPFLETLAAASGRPVVIAALLHNPALPEASFNDL
                     RAIGAAQARGRRLYGQVSCCALTNEFTLRSPYPFEGMAAWLPAMQAEGDALKRVYADP
                     AFRRRVSAELATPAAIRLFNGEWDKLRLLAAARPENRRHEGRSVAELAAASGQSPLDW
                     LLDFSLAEDLDTLFIATLLNSDEEAVGRMLRDPYASIALSDAGAHLTFFCDAGFGLHL
                     LGHWVRERGVLTMEEAVWQLSGRPAEIFGIRDRGRIAAGLAADLLLFDPATVGRGPSR
                     RVFDLPGGAPRLTTPALGVHGLWINGTQVADATGLIADEERSGRLLRDFAG"
     gene            complement(16728..17297)
                     /locus_tag="VGP50_09920"
     CDS             complement(16728..17297)
                     /locus_tag="VGP50_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEV8027738.1"
                     /translation="MGSAAIFAALTRGAPAAFRRRMAWRGPTIAGILILAFAFGGAAL
                     LRALGITLPAFRIAGGVLLFLLAVDMVFARPSGIRNPTAPEEAEAEHRADISVFPLAF
                     PLLAGPGALTSIVLLMGRAQGALAAAGVIVALLAVMLLTLLLLRAAPQVLRVLGVTGA
                     NVVSRVLGIILAALAAQLIIDGVSEALRR"
     gene            complement(17375..>18207)
                     /locus_tag="VGP50_09925"
     CDS             complement(17375..>18207)
                     /locus_tag="VGP50_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV8027739.1"
                     /translation="TEIRPGLIDILELPAWLSRLTFAPRGDAMLHEFDKVIDRLITSR
                     ERAPADGQRKDLLGRLIAARDEETGGGMSAQEVRDQVVTIFMAGHETTALALTWTWYL
                     LSQHPAAEAKLHAELDQVLGGRPPRHEDLAKLPYTRMVIEEAMRLYPPAHTLSRTAVK
                     EDVVSGHRIRPGAQIFIVPWLLHRNPKLWDRPDRFEPERFAPERAASRHRFAYIPFGA
                     GPRICIGAAFAMAEAMLLLATIAQRYRLRLKPGFAVEPQGLITLRARYGMRMLLERRG
                     "
BASE COUNT         2862 a         6043 c         6374 g         2928 t
ORIGIN      
        1 ccgtgtggct atcgccgata ccgccggcgc cgcctgggcg gtggcacgct gcggcagcgc
       61 cccggcggca gtggtgccgg aaggcggcgt gcgcgcggtg ctggcgccgc tgccggtgct
      121 ggccttgcgc ctcgatccgg cactggcggg cgaactcgag cggctgggcc tgcgccgcat
      181 cggcgatctc tatgccttgc cgcgcgcggc gctggtcacg cgcttcggcg agacggcgac
      241 gttgcgcctg gaccaggcgc tgggcgtcgc gccggagccg ctctcgccgc tgccgccggc
      301 gcccgcccgc tggacgcgcc gccgcttcgc cgagccgatc ggcacggccg aggcgatcgc
      361 cgcggcgacg cgcgtcttgc tggatgcgct gtgccggcgg cttgccgatg aggggctggg
      421 cttgcgccgg ctcgcgctcc ggctttaccg cgtcgatggc agtagcgcgg aagcggcaat
      481 cggcacggca cggccgagcc gtgatgtccg ccatctcttg cggttgctcg aggagaagct
      541 gcccgcgatc gatcccggcc tcggtatcga ggacatgctg ctcgaagcgg cccgggtcga
      601 gcggctggcc gcggcgcagc tgtcgttcag aaatgcgctc catcatttcc ccgtcgtccc
      661 tgcgaaagca gggacccagg gcgagcgacg tgcggttgcc ctgggccccc gctttcgcgg
      721 gggcgacgat ggagagaaag cgaaagggag cgtcgacgaa accgaggtcg cggcgctggt
      781 ggatcggctg gccaatcggc tggggccggg gacggtgttc gcgatcctgc cgcgggagag
      841 ccatgtgccg gagcgggcgg tgcgccgcgc gcctgtcttc accacgacga aagcggcggc
      901 ctgggatccg cagcgcctgc gccccatccg cctgctgccg cggcccgagc cgatcgaagc
      961 ggtggcgccg atcccggacg atccgccgat cctcttccgc tggcgtcgcc tccagcacca
     1021 gatccgccgt gccgacgggc ccgagcggat tctcggcgag tggtggcgca gtgccgcgga
     1081 agcggaggag ctgcgcgact attaccgcgt cgaggacgag gcgggccgcc gcttctggct
     1141 ctatcgcctc ggcctctatc gcccggaagc gccgccccgc tggttcctcc atggactctt
     1201 cgcctgaggt catggcccaa tacgccgagc ttcaggtcac cagcaatttc tccttcctgc
     1261 gcggggcgtc gcatccccat gagctggtgg cggaagcgaa ggcgatcggc catggcgcga
     1321 tcgccattgc cgatcgcaat acgctgggcg gcgtggtgcg cgcccatgcc gcggccaagg
     1381 agctgggctt ccgcctggtg atcggctgcc ggctcgatct cctggatgcg ccgagcctcc
     1441 tgtgctttcc caccgaccgt gccgcttatg gccggctcgc gcggctgctc acgctcggca
     1501 agcggcgcgc ggagaaaggc gggtgtcgcc tcgcctatgg cgacgtggtc gcgcatggcg
     1561 cggggcaggt ggtcgtcgcc gtggcgccag aggacgcacc ggatttcgcc gacttcctga
     1621 agcgcgtcgc cggcgatttt ccggacgcgg cctatctcgc ggcccagcat ctctatggcg
     1681 gcgacgatgg ccggcgggtg catcgcctgg cggcgctcgc ggagcgcgcg cggctgccgc
     1741 tgctcgccac caacgacgtg ctctaccacc ggcgggaacg ccgcgcgctg caggatgtga
     1801 tgacctgcat ccgcgaaggc tgcaccatcg ccgaagccgg cttgcgcttg aacgctcatg
     1861 cggagcggca tctgaagccg gcggccgaga tggcacggct cttcgcgagt tatcccgatg
     1921 cggtggcgcg cagcctcgag atcgtcgagc gctgctgctt cagcctcgac gaactgcgct
     1981 atgaatatcc cgacgaggcc ggaaacgacg ggacgccggc acagccgcgg ctcgaacgcc
     2041 tgacttggga aggggcccgg cggcgctatc cccaaggcgt gccggagaag atccgcaagc
     2101 aggccgagac ggagctcagg ctcatcggcg agctcggcta cgcgtcttat ttcctcaccg
     2161 tccaggacat cgtcgccttt gcacggggtg agggcattct ctgccagggc cgcggctcgg
     2221 cagcgaactc gacggtgtgc tactgcctcg gcgtcaccgc ggtcgatccc gagcaggtcg
     2281 atcttctctt cgagcgcttc gtcagcaccg agcgcaagga gccgcccgac atcgatgtcg
     2341 atttcgagca tgagcggcgc gaggaggtaa tccaatacat ctatcggaaa tacacacgcg
     2401 accgtgccgg cctaacggcc gtcgtcagct cctatcgctc gcgcggcgcg atccgcgagg
     2461 tcggcaaggc gctgggcctt tccgaggatg tgacggcggc gctcgccggc accgtctggg
     2521 gctggagcga cgacgacgtc catgaggagc aggtgaaaag cgtcggcctc gatctcgccg
     2581 atccgacgct gcgccgcgcc ctcgagctcg cgggcgagct catcggcttt ccgcgccatc
     2641 tctcgcagca tgtgggcgga tttgtcatca cgcgcggccg gctcgacgag ctggtgccga
     2701 tcgagaatgc ggcgatggag gagcgcaccg tcatcgaatg ggacaaggac gatctgaacg
     2761 tcctcggcat tctcaaggtc gatgtgctgg ggctcggcat gctgagctgc ctccggcgcg
     2821 gcttcgcgct cctgaagcag cacaaaggcg aggatctatc gctggccacg attcccaagg
     2881 agcggcccga ggtctacaag atgctgggcc atgccgattc gatcggcgtc ttccaggtcg
     2941 agagccgcgc gcagatgagc atgctgccgc gcctccagcc caagaacctc ttcgatctcg
     3001 tcgtcgaggt ggcgatcgtc cggcccggtc cgatccaggg caacatggtg catccctatc
     3061 tccgccgccg caccgggcag gatccggaca cgtgtccgcc ggcgctcgaa gaggtgttga
     3121 agccgactca tggcgtgccg cttttccagg agcaggcgat gcgcatcgcc atgatcgccg
     3181 cgggtttctc ggcggtcgag gccgatggct tgcgccgtgc catggcgacc ttccgccgga
     3241 acggcgacat ccatcgcttc gaggagaagt tcatcaaggg catgctcgac aacaactacg
     3301 agcgcgattt cgccgagcgc tgcttccagc aaatcaaggg cttcagcgat tacggcttcc
     3361 cgctctcgca tgccgcgagc ttcgcgcttc tcgtctatgc ctcggcctgg atgaaatgct
     3421 tccatcccga ggtcttcgcc gcggcgctcc tcaacagcca gcccatgggc ttctacgccc
     3481 cggcgcagat cctgcgcgac gccgagacgc acggcgtcgt gttgcagccg gtcgacgtca
     3541 accacagcga ttgggattgc acgctcgcgc gacgcgaagc gtcgtcgcgg gcgggcgaca
     3601 gcggttgctc ttgcaaccgc gaaagcccgc acagagggtg cgaatgcacc gagcctcact
     3661 gctcaaattc tcttcccctc tcccccacag cgtgggggag agggcagggt gagggggtgt
     3721 ctcagccaac tcggagccag cctgcgcatg tcgagacccc cctcacccta ccctctcccc
     3781 ccgcaagagc ggggggagag ggggaaattg gagggaaggc gagagttggc gcgcgtccga
     3841 ctcacgcgct ccgccttggc tctcgcctgg tgaaaggcct ggcgaaagag gaagccgaga
     3901 agatctcgga gaagcgtggc tcaggctatc gcagcgtggc ggagctgcag tatcgggcga
     3961 agctccatcg cggcgcgctc gagcggcttg ccgaggcgga tgcgctgcaa tccctgggtc
     4021 tcgaccggcg ccgcgcgctc tgggcggtga agggcttgcg cgacgaacag gagctgcccc
     4081 tcttcacgcc gctggccggc gtcggggtca aagaggcggc ggccgtcctg ccgcccatgg
     4141 ggctgggcga ggaagtggtc gaggattatg cggcgctcgg gctctcgctc aaatgccatc
     4201 ccgtggcgct gctgcgggat gcgctcgacg cgcggcgcat tctccggaac gggtgcctcg
     4261 aggtgacgcc agcggagaag cgggtgaggg tggcggggct cgtgctcgtg cgccagcagc
     4321 ccggcaccgc cagcggcatc atcttcatga cgctcgagga cgagactggg atcgccaaca
     4381 tcgtggtgtg gcgccgcgtc ttcgagcgtt atcgccgcgt cgtcatgggc gcgaagctcg
     4441 tcgaatgcca gggccgcgtg cagcgcgaag gcgaggtgat ccatgtcgtc gccgagcgcc
     4501 tcatcgatct ctcgccgctg ctccgccagc tctgggaccc cgaagcgcgc gagcgcccgc
     4561 cgctccgctt gcacagccgc gatttccgct gacgccgcgc gatattgccg ggcggccggc
     4621 ttcgtcttac gctttcgtcc aagaacaagc agggagggac gaatgccggt gatcaaggtg
     4681 acggacatcg ctcacggccg gctgcgcgcg ccggatctcg acgtcatgga agagttcctg
     4741 acgcatttcg gcatggtgag ggccgagcgc acgcccgccg cgctctatat gcgcggcacc
     4801 ggtcctgccc gccacatcca tgtgacggag aaaggcgagc ccgccttcat cggcctcgcc
     4861 tggcatgcgg ccgatgaggg cgatctgcgg cggcttgcga cattgccggg cgcgtcggcg
     4921 gtggagcagg tcgacgagcc cggcggcggc aaacgcgtcc gcctcaggga gcccaatggc
     4981 taccagatcg acgtgctcgc gggcgtgacg ccgctcgcgc cgcttgccgt ggcacgccag
     5041 gcggtgaaca ccggcgccga gacgctgaag cgcaagggcg agctctatcg cttgcgcgcc
     5101 ggcccgtcgc aggtgaagcg catcggccat ggcgtgatgt gcacgccgcg tctcgcggag
     5161 acggtgcgct ggttccgcga gacgctgggc ctcatcgcct cggacgacct ctatgccgga
     5221 agcaaggaca atctcatcgg cgccttcagc cgctgcgacc gcggcgacga gtatgtcgac
     5281 caccacgtct tcttctgttt gaagcacgcc aaggccgggc tcaatcatct gtccttcgaa
     5341 gtccaggaca tggacgacgt gttcgccggc catcactatc tgaagcgcgt cggcgaatac
     5401 gagcatatgt ggggcatcgg ccgccatctc ctcggcagca atgtctatga ctactgggcc
     5461 gatccctggg gccgcatcca tgagcattgg tcggatagcg atcggctcaa cgcgagaagc
     5521 ggcgcgaatt tgatgccggc ggaagagggg ctggtgtcgc aatggggtga gccgccgccg
     5581 cagaagctgc tcgaccgcgt cagcgcgtag ttttcgagtt gtcagtcgtc agttgtcagt
     5641 tcactgacga ccgaaaacag agcccgatcc ctgccgcctc ttcggagacg gagcggcagg
     5701 gacgggtcca cgcgtcaata gtgaagcagc cggctatttg tgagtctcgg cgtccttgat
     5761 cgcttgatcg gtcgcgtgca gggcggcctc gacatgctcg agcgaacggt gcttcaagcc
     5821 ttgcgcggtc ggatcgtcct cctcgcggtc gacgtcctga tggaccttgc gcaagagctc
     5881 cgccgccttg tggagacggc ccggatgatc tttcgggacg tcgacggccg gatggtcttt
     5941 gaggtccttg ccgtcatcaa tggaggcgcg cttgatctcg ccgagggcgg cgtcgatctg
     6001 ctggacggcg acgtcctcct ggccgctcac tgcggcgtct cctggacggt gctcgagcat
     6061 ccagcgcgcc gtgcgcaagt cgctgagcgc atggagatag tagggatgct cgcccggcaa
     6121 atccgcgcgc gccgcaagcg gcagcagcgc tacgacggca gcgaatagta tacgtgatgc
     6181 gaatcggtat ttcgacatgt cctatctctc ctgttcctgc tttgacccgc gttgagccgc
     6241 ggtgccggcg aaacttcagg agaacggagc agccgcagcg tttcatccgc gcgcggcgtg
     6301 gtatttttcg gccgcgcgta acggcggaga cgtcgctccg tcggtgcgtg agtatgcgca
     6361 catagatcgt cgtctttgct ttcgcgcgcg aaagcgagga cgacgaccaa aatgattctt
     6421 acgcgagcgc cttggcggag ccggtcgcgc tcgcggcggc gggcgcggcg atgacgcggt
     6481 cggtcgcggg atcgatgagg tgcatgtggc gcatgtcggc catgaagcgc atgcgctcgc
     6541 cgggtgcggc ggcggcctcg gggctgacgc gggcgcagac ctcgacgcca tcgaccagga
     6601 aatagaccat ggtctccatg cccatgggct ctaccacgtc gagcggcatg gcgaattcgg
     6661 cgccggtgcc gtagctgcgc gtttcggtga cgtgctcggg ccgcaagccg aggacgagag
     6721 ggcgctccag atgttggcga taagcggcgg ttcgctccgc cggcacgggg agcgacagat
     6781 cgtcggcgag gcgcaccgac aagccgcccg ccgcttcgac gagacggcag gagagaaaat
     6841 tcatcgcggg cgagccgatg aagccggcga cgaagcgggt ctgcggccgg tggtagaggt
     6901 cgtgcggcgt gccgatctgc tcgatgcggc cgccattcat gacgaccacg cgatcggcga
     6961 gcgtcatggc ttcgacctgg tcatgggtga catagatcgt ggtggtgcgc accttctggt
     7021 gcactttctt gatctccgtg cgcatctgca cgcgcagctt ggcgtcgaga ttcgacaagg
     7081 gctcgtcgaa aaggaagacc ttgggattgc ggacgatggc gcgccccatg gcgacgcgct
     7141 ggcgctggcc gcccgagagc tggcgcggcc gccttgcaag gagatcgatg atgtcgagaa
     7201 tgcgcgccgc gtgctcgacc cgcgtcctga tctcgtgctt ggggaatttc ttgaggcgca
     7261 gcccgaagga catgttctcg aacacggtca tatgcgggta gagcgcgtag ttctggaaca
     7321 ccatggcgat gtcgcggtcc ttggggggca ggtcgttgac cacctggccg ccgatcagga
     7381 tctcgcccgc gctcgtctct tcgaggccgg cgatcatgcg caaggtcgtg ctcttgccgc
     7441 agccggatgg accgacgagc acgacgaact cctcatcggc gatgtcgagg tcgatgccgc
     7501 gcaccacctc ggtctggtcg aatttcttgg cgacgctgcg cagcaccacc tgggccatcg
     7561 cttcatccct tggtcgcgcc ggcggtgagg ccggcgatgt aatagtccat gaggaaagca
     7621 tagatgacga ccggcggcgc cgccgcgatc agcgcggccg tcatgatcat gccccattga
     7681 tagacgtcgc ctttgaccag cgtcgtcacc gtgccggccg tgagcacgag ttggtcggag
     7741 ctgtagatga aggccatggg atagacgaag gccgcccagc agacggtgaa ggcgaagatg
     7801 gtggcggcga tgaggcccgg cagggcgacc gggatgaaga tcttgaacag catctgcaag
     7861 tgattggcgc cgtcgatcag cgcggcttcg tcgagctcct tggggatgct ctggaaatag
     7921 ccgatcatga tccaggtgca gaagggcacg gtgagcgtcg gatagacgag caccagcgcc
     7981 cagtaggaat cgaggaggcc gagcccgccc acgatcttga agagcggcag gaagagcagg
     8041 gagtccggca cgaggtaggt gaggaacacg cccgtcgcca gcacgcccga gccccagaag
     8101 ctcatgcgcc ccagagcgaa ggccgccggc accgcgatca gcatggtgat gatgacgacg
     8161 cagaccgtga cgatcgcagt gttctcgagg tagatcggaa agtcggtctt gttccagagc
     8221 gtcgcgatgt tgtcgagcgc caccggaatc gccggcagcc acggatccgt cgtctggttc
     8281 gagatctgcg cgctcgactt ggtcgcggtg acgagttggt aatagggcgg gatgagaaag
     8341 aagatcgcga agaggacgag gaagaaatag gaggcgacga gggcccaccg gcgattgcgc
     8401 cggaggctgc cgctcgagcg gcggcgcggc gtgtcggcgg cgagcgcggc catcagatct
     8461 ccctgccgcg ccggcgcacg ccgcgcagga tgaagatcgc ggagatgccg aggatcggga
     8521 acatgaagag cgacacggcg gcgcccaagg gaatgtcgcc gctggcaatg ccgagctgga
     8581 acgcccaggt ggcgaagaca tgcgtgctgt cgcgcgggcc cccgccggtc agtacgcgga
     8641 cgatgtcgta attcgcgaaa gtgacgatca gcgagaacag cacggtgatg gcgatgatgt
     8701 tgcgcatcat cggcagcgtg atgtggatga atttctgcca ggcgttggcg ccgtcgatgg
     8761 acgccgcctc atagagctgc tccggcaccg atttgagcgc cgccagatac ataattagaa
     8821 agaagggcga gccgtaccag acattgacga ggatggtcga gaggcgtgcc caggccggcg
     8881 cgctgagcca gggaatctgc ggcgcgccga tttgcgcaaa gaaccagttg aaggcgctgt
     8941 aggtggggtc gagcagccag agccaaccga gggtcgagag cgagagcggg atcacccagg
     9001 gcacgagcag catgccgcgc catttgcgtt gtcccttgcc cggcaaattg ttgacgagat
     9061 gcgccgtgac gatgccgatc agcgctttga acagaacggc ggtccccgcg aaaatcgcgg
     9121 attgctcgac caccatccag aacgtctcgc gcccgaagag gaagcggtaa ttggcgagcc
     9181 ccacgaatct ggtctgagtc ttgttgagga gcgagagata gatcgcgtaa agaccgggat
     9241 agacgatgag cccgagcacc accgccagaa gcggcaccgc gaacaggaag gcgatcgtcg
     9301 agcgccgccg cgcaaaacgc cgcagcccac ccggccgcgc ttcggcgcga gggatgggga
     9361 ggcggcggga gaggacgtcg gtcataccag agacaacgca aagcattccc tctcccctcg
     9421 cgggagaggg aggggcccgg cgcgccagcg ccgggagggt gagggggctg gcgctggatc
     9481 gagagctcgg cgtcgatgcg tctgcgtctt acccctcacc ctcccatcgc ttcgcgatgg
     9541 gtccctccct ctcccgcaag gggagagggg aagaggacgg ggccgggtgg gcgcggacat
     9601 aagcgcctca gcttctcaga tacccctcgc attctcgcgc ggcccatttg acgacgtcgt
     9661 cgatcttctc gccgccctgg gccaccctgg cgatcatgac cggctgcagt gcctggttgt
     9721 agagctgcgc ggccacgtcg ggtcgcgccg gatatccggc gacgctgagc tcctcgtcgc
     9781 cgcgcggcgg gtagttgaag acggtgccga gcggcggctc gacggtgcgc caggtgtcga
     9841 aatcgtaata gctcttgaag gaagggagat cgtagcccga ggaggccgcg acctggcgtt
     9901 tcgcctgcgg cttgtcgacg atgtggtgga tgagctcctt ggccgcgctt ttgttctggc
     9961 cgaaggccca ggcgccatag aaccagggca gatagggtgc aaagcggccg ttggcaccgc
    10021 gtgggatgtc atgggtccag cattgctcgg cgaccttggg gttgtcgcgc ttcgccacgg
    10081 cccaggcgct gggcgggttc atgatgctcg cgcccttgcc ggagatcagc catttgttgt
    10141 tgccggcatc gtcccaggca tagacctcgg gcgggttctg cgccatgagc ttccgcgcgt
    10201 attcgaggac ggcgcgggtc tcgggggagt cgacgcggat gttgtccttg tcgtcgacga
    10261 agacggcgcc gaaggagcgg aagagggcgc ccacccagtc gatcgaatca ctggtctggc
    10321 ccatgggcag gcccacagga taccccgcct tgaacagctt ctcggccgtc gcgagataga
    10381 gatcccagtt ccagctgtcg atcagcgccg gatcgcgctt gtcgtcgaag gggaagacct
    10441 tcgtcacatc gaggccgcaa tgctgcttgt agaggtcgat gcgcgagcag caaggcttga
    10501 cctgactgcc ggcgatggcc ggcacggcct tccagctgtc ctcgtctcgc gccaggtact
    10561 cgctgatcgg cgcaatcggt ccatgctctt tgaccagcct gtcgacaacg tcgttcatcg
    10621 gctccagcac ggtgcggtgg acttggacct gccaggagcg atgggcgatg acgtcgtggc
    10681 cggtgcgcgc ctgcgcttcg gcgctcgccg tcatgatgtc cttgtcgccc tgcgaggtga
    10741 tgaaatcgat gtgcacctcg acgtggttct tctcgcccca ttcgttgcag atggccgcca
    10801 gcacctcgtt ggcacccggc acccaatggt cccagcagcc gagcgtcagc cgtcccgccg
    10861 aataggagct gcgcatatag ggcgcggcga tggcggccgt ggtcgccagg acggcccgct
    10921 tcaggaaccg gcggcgcgtg agcgcggttt tcatgtgtcg aacctcccgg cgagaccgct
    10981 cttggcggcg gtccttcgat tgctgacgcc atcatgatcc ttgtgttgcc ggttgggcaa
    11041 gggtgccgtc ggaccgctca ggccatgagg tcggcgagct cccgcagggt cggcaccagc
    11101 agatcgggct gatggggcgt ggctccgaag ggccgccggc ggcggtcgat gaaagccgtg
    11161 cgcatccccg ctgccttggc gccaatgcag tcgaaggcat ggttggcgac gaacagcacg
    11221 cggctccgct cgacgccgat gatctccgcg gctttcgtgt aggtcgccac atgcggtttg
    11281 aagctgttgg cgacggcaac ggagatcagc ttgtcgaaag caaggccgtg atgcggtttc
    11341 gccgtttcga gcatgtcggg atcgccgttc gacagcacgg ccaggcggta gcgccggcgc
    11401 agccgcgcca gcgcgtccgg cacttcgggg aagggccgca ggcgctcgat ctcggcgacc
    11461 agggcgcgca cgtcctccat gcggtagggg atgcccgccc ggtccagcac ataagcgagc
    11521 gcccgctggc cgatctcgcg atagggcgta tgctccttgt gcagcagcgc gtcgatcatc
    11581 gaattctcga aatgcgtgcg ccgccaccac gtcacgagag acgatgggtt gcccgcccag
    11641 cccttggctt cgaggaaggg cgtcacggcc tgggtgagcc cggtctggat gtcgaccacg
    11701 gtgccgtatt ggtcgaacat gcagacctcg atcgtcgatt tcagcgcctc cacatccgcc
    11761 atcgcttgcc ctcccatgtg gcgttaaccc acctatgata ggggatcggg ttccgagcgt
    11821 cggagcccgc cgaggatcgc ccatggcttt tgtattgccc gcgccactgc ccgctccgga
    11881 tccgctgcgc cgcacgctgc gcgccgtcta tcgcgcggac gagacggaga cggtggagcg
    11941 catcgtcgca gcagccaccc tgccggagga gataaacgcg cgcatctccg cgcgtgcgcg
    12001 tgacctggtc gccgaggcgc ggcggcgccg gctcggcaag ggtggtatcg atgccttgct
    12061 ccatgaatat gcgctctcga cgcccgaggg cgtcgccctc atgtgcctcg ccgaggcttt
    12121 gctgcgcatt cccgacgccg ccaccgtcga ccggctgatc cgcgacaagc tcgcgccggc
    12181 cgattgggcg cgtcatctcg gccattcgga ttcgctgctc gtcaacgcgt cgacctgggg
    12241 cctcatgctc accggacggc tcctcggcag caatgccgat gacggcgatc tcgcggcggc
    12301 gctgcgccgc ctcctcgccc gctcgggcga gtcggtggtg cgccaggcgg tgaccgcggc
    12361 gatgcgcgtg ctggcgcggc aattcgtcat gggccgcacc atcgcggaag cgctcgagcg
    12421 ggccgaggcg cccgagcgcg agggctatcg ccattcctac gacatgctgg gcgaagcggc
    12481 gcggaccgcg gccgatgcgc ggcgctattt cgagagctac gacgcggcga tcgcggccat
    12541 cggcgcgacc gtcgccggcc gcgacatcgc ggcggcgccg ggcatctcgg tgaagctgtc
    12601 ggcgctccat ccgcgctacg aggaggcgca gcgggcgcgg gtgctgcgcg agctcacgcc
    12661 ggtgctgacg gcgctggcgc tccgcgccaa aaaggccggc atcggcttca ccatcgacgc
    12721 ggaggaggcc gaacggctcg agctttcgct cgatctcatc gcggcgctcg cgctcgatcc
    12781 cgcgctcgcc ggctggaacg ggctcggcct tgccgtccag gcctatcaga agcgtgcgct
    12841 gccgctcctc gactggctcg cggatctcgc gcgccggagc cggcggcggc tgatgctgcg
    12901 gctggtcaag ggcgcctact gggacagcga gatcaagcgc agccaggagc gcggcctcgg
    12961 cggctatccc gtcttcaccc gcaagatgtc gacggatgtt tcctatctcg cctgcgctcg
    13021 gcacatcctt gccgcgcccg acgctttctt cgcgcaattc gccacccata acgcgcacac
    13081 gctggccgcg gtgatcgagc tggccggcga gcggagcgac tacgagttcc agcggcttca
    13141 cggcatgggc gaggcgcttt acgagcaggt ggtcggcgcc gagaagctcg gccggccctg
    13201 ccgcgtctac gcgcccgtcg gcagccatga ggatctgctc gcgtatctgg tgcggcgcct
    13261 cctcgagaat ggcgccaata cgtccttcgt gaaccggctc gtcgacgagc aggcgccgac
    13321 cgaggagatc gtcgccgatc ccgtggcgcg cgtcgcggcg ctgaaggaaa agccgcatcc
    13381 acgcatcccc ttgccgcgcg acctctacgg acccgagcgg cgcaacgccc gcggcatcga
    13441 tctttccaat cccgcgcggc aggcggcgct ggccgaggcg atggaagcgg cggctgggcc
    13501 gtggcaagcg gcgccgctca tcggcggcac cgagcgcccg ggcccagccc gcgcggcgct
    13561 cgatccggcc gatcgccgcc gcgagatcgg cacggtcagc gagaccacgc cggagcatat
    13621 cgagacagcg ctggcccagg ccgccgcggc ggcgcccggt tgggatgccg tgccggcgcc
    13681 ggcgcgcgcg gccgctctcg agcgcgccgc cgatctcctc gagacgcgcc tggccgattt
    13741 catggcgctc atcgtgcgcg agggcgggcg cactatcccc gacgcactgg ccgagctgcg
    13801 ggaggcggtc gattattgcc gctactacgc gctctgcgcg cgcgccgact tcgcggcgcc
    13861 gatcgtgctg ccgggaccga ccggcgagcg caacacgctc gcactggcgg ggcggggagt
    13921 cttcgcctgc atcgcgccct ggaatttccc actcgccatc ttcatgagcc aggtcgtcgc
    13981 cgcactcgcc gccggcaatg ccgtgatcgc gaaaccggcc gagcagacgc cgctcgtcgc
    14041 cgccgcggcg gtgcgccttc tgcacgaagc gggcatcccg ggcgatgtgc ttcacctttt
    14101 gcccggcgac ggcgccagcg tcggcgcaac gctggtccgc gatccgcgcg tcgccggcat
    14161 cgccttcacc ggctcgaccg agacggcgcg ggcgatcaat cgggcgctcg cggagcggcc
    14221 gggcccgatc atcccgttca tcgccgagac cggcggccag aacgcgctca tcgtcgattc
    14281 atcggcgctc cccgagcagg tggtgggcga cgccgtcgcc tcggccttca acagcgccgg
    14341 ccagcgctgc tcggcgctcc gcgtcctctt tctccaggac gacatcgcgc cgcgcgttct
    14401 cgccatgctg gagggcgcca tgcgcgagct cgcgatcggc gatccgggcc tgctctccac
    14461 cgatgtcggg ccggtgatcg acgatgctgc gcgctcagca ctcctcgcgc atgcggcgcg
    14521 gatgtcgcgc gacggccggc tcatcgccga gctgccgctg cccgccggca ccgagcacgg
    14581 tttcttcttc gcgccgcgcg ccttcgagat cgatcgcctc gatcttctcg agcgcgaggt
    14641 gttcggcccg atcctccatg tcatccgctg gcgcgccgac gcgctcgacc cggtgctcga
    14701 tgccatcgcc aagaccggct atggcctcac gctcggcatc cacagccgca tcgacgacac
    14761 cgtgcggcgc attcatcagc ggcttcgcgt cggcaacact tacgtcaacc gcaacatgat
    14821 cggcgccgtg gtcggcgtgc agcccttcgg cggcgagggc ctctccggca ccggccccaa
    14881 ggccggcggt ccgcattatc tccaccgctt cgccaccgag cgcaccctca gcatcgacac
    14941 gaccgcctcg ggcggcaacg ccacgctgct gtcgctgcag gaggaggcgg gttaagaacc
    15001 tctccgggat tgtccggcgc gtgaggcgca gcaagcatgc atgatctcgt catcaagaat
    15061 gccctcgtct tcgacggcag caaagcgcca ggccggcatg tcgcggtggc cgtggcgcaa
    15121 ggccgcatcg ccgccgtcga cgcggatgtc ggcgccggcc gccgcgtcat cgacgccgac
    15181 ggtctggcac tcgcgcccgg gatcatcgac acgcataccc actacgatgc ccaaatcacc
    15241 tgggatgccg gcgttcaccc gtcgcccgag ctcggcgtca gcacggtcgt gatcggcaat
    15301 tgcggcttca ccatcgcgcc ctgccgcgcc gagcaccgcg acctcaccct gcgcaacctg
    15361 acccatgtcg agggcatgtc gctcgcggtg ctgcgaagcg gcatccgttg ggagttcgag
    15421 accttcccgc aatacctcga cgtcatcgag cgttcgggca gcgtccccaa cgtcgccgct
    15481 ttcatcggcc attcctcgct gcgcgtctgg gtgatgggcc gcgccgcgac cgaacgcgcg
    15541 gcgagcgcgg ccgagatcga cgcgatgcgg cgcctgttgt gcgaagcggt cgatcggggc
    15601 gccgtcggct tcgcgacctc gacggcgccg caacacaatg gcgagggcgg cacgcccatg
    15661 ccgtcgcggc tggccgatga aagcgagctg cgcgcgctcg tcggtgtgct cggcgatgcg
    15721 ggtcgcggtg tcttcatgct gacgaagggc atgagcacac ccgtgccgtt cctcgagacg
    15781 ctggcggcag cgagcggccg ccccgtggtc atcgccgcgc tcctgcacaa cccagcgctg
    15841 cccgaggcca gtttcaacga cctgcgcgcc atcggcgcgg cgcaggcccg gggacggcgg
    15901 ctctacggcc aggtgtcgtg ctgcgcgctc accaacgagt tcaccctgcg ctcgccctat
    15961 cccttcgaag gcatggcggc gtggttgccg gcgatgcagg ccgagggcga tgcgctgaag
    16021 cgcgtctatg ccgatcccgc cttccgccgc cgcgtcagcg cggagctcgc gacgcccgcc
    16081 gccattcgcc tgttcaacgg cgaatgggac aagctgcgcc ttttggcggc ggcacggccc
    16141 gagaaccggc gccatgaagg ccgcagtgtt gccgagcttg ccgccgcttc gggccagtcg
    16201 ccgctcgact ggctgctcga tttcagcctc gccgaggatc tcgacacgct cttcatcgcg
    16261 acgctcctca acagtgacga ggaagccgtc ggccgcatgc tgcgcgaccc ctatgccagc
    16321 attgccttgt ccgatgccgg cgcgcatctc accttcttct gcgatgccgg gttcgggctc
    16381 catctcctcg gtcattgggt gcgcgagcgg ggcgtgctca ccatggagga ggcggtgtgg
    16441 cagctctccg ggcgaccggc cgagatcttc ggcatccgag accgcggccg catcgcggcc
    16501 gggctcgccg cggatcttct gctgttcgat ccggcgacgg tcggacgtgg cccgagccgc
    16561 cgcgtcttcg acctgccggg cggcgcaccg cgcctcacca cgcccgcgct cggcgtccac
    16621 ggcctctgga tcaacggcac acaggtggcg gatgcgacgg gcctcatcgc cgacgaggag
    16681 cggtcagggc ggctgctgcg cgacttcgcg ggctagcgct tcgtgctcta tcgccgcagc
    16741 gcttcgctga caccgtcgat gatgagctgc gcggcgagcg cggcgaggat gatgccgagg
    16801 acgcggctca cgacattggc gccggtcacg ccgaggacgc gcaacacctg cggcgctgcg
    16861 cggaggagca gcagcgtcag cagcatcacg gcaagcagcg ccacgatgac gccggcggcc
    16921 gcgagcgctc cttgcgcgcg ccccatcagc agcacgatcg aggtgagcgc gccaggcccg
    16981 gcgagcagcg ggaaggcgag cgggaagacc gagatgtcgg cgcgatgctc ggcctcggcc
    17041 tcctcgggcg cggtcgggtt gcggatgccg gacggccgcg cgaagaccat gtcgaccgcg
    17101 agcaaaaaga gcagaacgcc gccggcgatg cggaaggcgg ggagggtgat gccgagcgcg
    17161 cgcagcagag cggcgccgcc gaaggcgaaa gcgaggatca ggatcccggc gatggtcggg
    17221 ccgcgccacg ccatgcgccg ccggaacgcg gcaggggcgc cgcgcgtcag cgccgcgaaa
    17281 atcgccgcgc tgcccacggg gatcgatgat cacgaaaagc gtgacgaagc tgtagagaac
    17341 ctgttccagc acggggcgcc cgacgccgcg gcggtcagcc gcgccgctcg agcagcatgc
    17401 gcatgccgta gcgcgcgcga agcgtgatga gcccctgcgg ctcgacggcg aagccgggct
    17461 tgagccgcag acggtagcgc tgcgcgatgg tggcgagaag cagcatcgcc tcggccatgg
    17521 cgaaggccgc accgatgcag atgcgcggac ccgcgccgaa ggggatgtag gcgaagcggt
    17581 ggcgcgaagc ggcgcgctcc ggcgcgaagc gctcgggctc gaagcggtcg gggcgatccc
    17641 agagcttcgg attgcgatgg agcagccagg ggacgatgaa gatctgcgcg ccgggccgga
    17701 tccggtggcc ggagaccacg tcctctttca ccgcggtgcg cgacagcgta tgcgccggcg
    17761 gatagaggcg catcgcttcc tcgatgacca tccgcgtata gggaagcttc gccagatcct
    17821 cgtgccgcgg tgggcgtccg ccgagcactt gatcgagctc ggcatggagc ttggcttcgg
    17881 ccgccgggtg ctgcgacagc aagtaccagg tccaggtgag cgccagcgcc gtcgtctcgt
    17941 ggccggccat gaagatggtg acgacctggt cgcgcacctc ctgcgcgctc attccgccgc
    18001 ccgtctcctc gtcgcgcgcg gcgatgagac ggcccagcag gtccttgcgc tgaccgtccg
    18061 ccggcgcgcg ctcgcgcgag gtgatgaggc gatcgatgac cttgtcgaac tcatgcagca
    18121 tggcgtcgcc gcgcggcgcg aaggtgaggc gcgagagcca ggccggcagc tccaggatgt
    18181 cgatgagccc tgggcggatc tcggtct
//
LOCUS       DASYNS010000321        10406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5070365, whole genome shotgun sequence.
ACCESSION   DASYNS010000321 DASYNS010000000
VERSION     DASYNS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10406
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5070365"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VGP50_09930"
     CDS             complement(<1..318)
                     /locus_tag="VGP50_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027740.1"
                     /translation="MRELGVQLKVSADFDRLIALNRQHRDSWPLLSPIFNPKFNDLAA
                     GKAFLIEGIDRAGDTVITSAGRLYDHGTRSLADDLRSLRVFYDHPAPHVAAGARVEVD
                     ASSA"
     gene            712..1467
                     /locus_tag="VGP50_09935"
     CDS             712..1467
                     /locus_tag="VGP50_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV8027741.1"
                     /translation="MSKFSASISGRLEQERRLAGVSQESLAEVLRVSAETIGRYEAGE
                     EWLPADQLATAVAFLGISLSALFYDCASAKSRKEIASDRWIAIRRPLNILSTGDFDEV
                     APLVSLWEDKRGELTEDIEDALLISGMLDRVVILRRPRKSARLVAHHFGSRIVFARPA
                     DIVDRDIQHLPDRDYGALVADAYDEALWHGTPLVEANRAIIGTWDGKTIGARYDRVLL
                     PWRWRSSDQFVMCISLRRGQLAQMDSAAAVLPP"
     gene            1715..2449
                     /locus_tag="VGP50_09940"
     CDS             1715..2449
                     /locus_tag="VGP50_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027742.1"
                     /translation="MREERRLAGATRETLGEALGVSSEIIEQCESSGKLLPAHHLATA
                     AAVLRVSVSALFYNGAERGAPAAKRREYQRWLAISRPPKFLYRPDFAGLAPLLAFWQE
                     RRGEFSAEVDSALSAGGILERMILVRRSPDSSRLETEHFGAKFMFLRPSDIVGREFQA
                     QPDQEYAAWMIEAYEQLLDRPELELRVEACRAIIHAWDNTTICARYDRVLIPWRRRGS
                     DQFLMCIALRRGEPAAVPSDTDALSP"
     gene            2446..3732
                     /locus_tag="VGP50_09945"
     CDS             2446..3732
                     /locus_tag="VGP50_09945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8027743.1"
                     /translation="MTSGPRSAESAARFRRGWRERFDWHARRGGAAGIIAVLTATQWL
                     NWSSTYYLMTVLARPIAAETGWSLAFVVTGLSLGLVTAGLVSPRVGRAIERHGGRPML
                     ALGSLLLAVGLLGVGLSRHPIGYLAAWIVIGVAMAASLSDAAFATLGRFYGLRAKTII
                     SNLMVLIAFSATFSWPFTALLTEALGWRGTCFCYAAMHLAIGLPMHLFLLPKTATLPV
                     ELSPSVPVRAETASFQAVQGAGPDRRTLLVWLVGTNITLQIAIGSVLAVHLLSLLETL
                     GVAYAAAVGFGSVIWLSQAGGRVIDALFGRRFHPVLEGVVASLLVLLGIALLLAAPPS
                     ALLVGIVLFGVGNGVRGILKGTLPLVLFGAEGYATLIGRLGLPTLIAQAAGPALGAVA
                     LAQWGAMPLLAILVALAALNLGLAAALWMATPRLAR"
     gene            complement(3749..4003)
                     /locus_tag="VGP50_09950"
     CDS             complement(3749..4003)
                     /locus_tag="VGP50_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027744.1"
                     /translation="MQGKRSTVRDVAAGIFVFAALAMPFASMLWDSAARMKVHFRTSE
                     METPRAFSTETSRSVCTVMAAALSYEDPSTGEYTHVACSE"
     gene            complement(4136..4654)
                     /locus_tag="VGP50_09955"
     CDS             complement(4136..4654)
                     /locus_tag="VGP50_09955"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV8027745.1"
                     /translation="MMKAEDLREMQAPFKALYREAPEKAVITLKAKGRLGEEGITCKV
                     ETGRALAEAGLHPATGGDGLSLCSGDMLLEALVACAGVTMKAVATALGLPLKGGAVSA
                     EGDLDFRGTLGVAKDAPVGFREIRLSFDIDCPATPEEIATLLRLTERYCVVFQTLAKP
                     PALKIAHRIGTA"
     gene            4768..5997
                     /locus_tag="VGP50_09960"
     CDS             4768..5997
                     /locus_tag="VGP50_09960"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEV8027746.1"
                     /translation="MQEEFYRIKRLPPYVFAEVNALKAKARAAGRDVIDLGMGNPDGA
                     TPEHIVKKLLEAVQNPKSHGYSVSRGITGLRRAYAAYYARRFDVTLDPEREVITTLGS
                     KEGLANLAQAITAPGDIVLVPNPCYPIHAFGFIMAGASLRHVPIGPSVDFMGELKKAV
                     MHSVPPPIAIVLNFPANPTAQVVGLDFYAEVVDFCRHHNIFVLSDVAYAEIYFDGNPP
                     PSVLQVPGARDIAVEFSSLSKTYSMAGWRIGFAVGNARLIGALARVKSYLDYGAFTPI
                     QVAATAALNGPQDCIAEIRERYKARRDVLIEGLRQAGWEVPSPPASMFAWAPLPPRFQ
                     EMGSLAFSKLLLEQANVAVSPGVGFGEYGEGYVRIALVENRQRLRQATRNIKQFLSGR
                     NSAAGQTRAQPIAATPA"
     gene            6334..7104
                     /locus_tag="VGP50_09965"
     CDS             6334..7104
                     /locus_tag="VGP50_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV8027747.1"
                     /translation="MPPLDAVTIRQWLWGRGFYLPGDARHVLELVRPLALAPAMTLLD
                     VAAGLGGPAWAIADEFGTTVSALERDHELTRRGETIASGMAKPAGVKVFDPERFELRG
                     GAFDRVLAREATYMVDDKERFLRVLSLALRPDGRLLMTDFVIEPSNAEQPAQAAWAAL
                     QPHRPRLWGLRQYSDCLRGLGLDLRAPEDMTAAYKAQILRGWDNLLQTVDLRSLPRAH
                     RTAVVDEAERWMRTVAALDAGTLKFYRFTALAGSALRR"
     gene            7205..7330
                     /gene="ykgO"
                     /locus_tag="VGP50_09970"
     CDS             7205..7330
                     /gene="ykgO"
                     /locus_tag="VGP50_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEV8027748.1"
                     /translation="MKIANSLKSLKKRDKNCRIVRRKGRVYVINKTNPRFKARQG"
     gene            7446..8933
                     /locus_tag="VGP50_09975"
     CDS             7446..8933
                     /locus_tag="VGP50_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV8027749.1"
                     /translation="MRMPVPDAALIARRAEIAAALARMVPPEAVIQNEDERRVYESDG
                     LTAYRQPPLLAVLPSTTEQVARILRYCRENAVKVVPRGAGTSLSGGALPLADGIILGL
                     GKFNRILDIDFANRCVVAQPGVTNLAVTTAVQHQGFFYAPDPSSQIACTIGGNVAENS
                     GGVHCLKYGLTTNNLLGIEMVLMSGEVLRLGGKHMEAEGYDLLGVMTGSEGLLGVVTE
                     VTLRILRKPATARAILIGFPSNESAGNCVAAIIAAGIIPGGMEMMDKPAIHAAEDFVH
                     VGYPLDVEALLIVELDGPAVECDHLMGRVEAIAREQGAVSLRVSNSEAERLAFWAGRK
                     AAFPAVGRISPDYYCVDGTIPRKRLPDVLTRMRELSQKHGLRVANVFHAGDGNLHPLI
                     LYDANNEGELERAEAFGADILRFCVEVGGVLTGEHGVGVEKRDLMPVMFDEVDLNQQQ
                     RLKCAFDPDGLLNPGKVFPELHRCAELGRVHIHRGATRFPDLPRF"
     gene            8942..10135
                     /gene="glcE"
                     /locus_tag="VGP50_09980"
     CDS             8942..10135
                     /gene="glcE"
                     /locus_tag="VGP50_09980"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEV8027750.1"
                     /translation="MTRFRPSDQRALTEIVQAAVAEEEPLEVIAGGSKRGLGRPIQVP
                     HILDVSAFSGISAYEPEELVLTAGAATPLAEIERMLAAAGQMLAFEPPDWRGLLGSDA
                     AAPTLGGTIAANLAGPRRVRQGAARDHFLGFHGVSGRGEAFKAGGRVVKNVTGYDLCK
                     LLAGSHGTLAVLTEVTVKVLPRAEAARSVALHGLDDAAALRAMTVALGSPHEVSGAAH
                     LPKGENGAGAPLTLLRLEGPEPSVKARAAALRQELAGAGAVEELSDAESARLWMAVRD
                     VAPLVAPRDRAVWRISVPPSAGPALAAGVARAVDLDYVFDWGGGLVWLAVKGAEDGGA
                     AEIRRRVAQSGGHATLIRAPDALRLAVPVFQPLPSALAALTARVKDGFDPKRILNRGR
                     MYSEL"
BASE COUNT         1629 a         3445 c         3490 g         1842 t
ORIGIN      
        1 agcggaggat gcatccacct cgacgcgcgc accggcggcg acatgaggcg cgggatggtc
       61 atagaagacg cgcaatgacc gcaggtcgtc ggcgaggctc cgggtaccgt gatcgtagag
      121 ccggcccgca ctggtgatca cggtatcgcc agccctgtcg attccctcga tcaagaatgc
      181 cttgccggcg gcgaggtcgt tgaatttggg gttgaagatc ggcgagagca gcggccagct
      241 gtcgcggtgc tgcctgttga gcgcaatcag gcgatcgaag tccgcggaaa ccttgagctg
      301 cacacccagc tcgcgcattt gctcctcggc gaatgcgaca tagcgaacga gaaggtcacg
      361 cggcccgtgg acaaaagtga tctgatcgaa cagcgtcggt cggccatcat acatagcaca
      421 cctccggggc atgtcgtggg agaggcgcca ttgggcctct tcggaaaggc gacatcgtag
      481 agagacgttg gtgaacgacc gcttacaagt gcataaaaat cgtctactac tattaggaga
      541 ggcgagattt ctaattatcc gccgatcgca acgagggttt gattgcgccc ggcggcagcg
      601 agcaacggct ggtggtatcg gtgcagtgcg aatgctaggc tgctggtcgg gtgcgtgcag
      661 ttcggggatg gcgatggtgg cggagcggcg agaagacgcg gtgaacttca cgtgagcaaa
      721 tttagtgcat cgattagcgg tcgcctcgag caggagcgcc gtctcgccgg agtgagccaa
      781 gagtccttgg cagaagtgct gcgtgtcagc gcggagacca ttggacgata tgaggcaggc
      841 gaagagtggc ttccggccga tcagctcgcc accgccgtgg cgttcctcgg catctcacta
      901 tcggccctct tttacgattg cgcatccgcc aaatcgcgca aagagatagc gtctgatcga
      961 tggatcgcta tacgccggcc gctcaacatt ctctccactg gcgatttcga tgaagtggcg
     1021 ccgctcgtga gcttgtggga agacaagcgc ggcgaattga ccgaggacat cgaggacgcc
     1081 ctgctgatca gcggcatgtt agatcgcgtg gtcattttgc ggcggccgcg gaaatcggct
     1141 cggctcgtcg cccatcactt tggctctcgg atcgtctttg cgcgcccggc ggatattgtc
     1201 gaccgagata tccagcattt gccggaccgc gactatggag cgttggtcgc agacgcctat
     1261 gacgaggcgc tatggcatgg tacgcccctc gtcgaagcca accgcgccat catcggtacc
     1321 tgggatggga agacaattgg cgcgcggtac gaccgcgtac ttcttccctg gcgttggcgc
     1381 agctccgatc agttcgtgat gtgcatctcg ctcagacgcg gccagcttgc gcagatggat
     1441 tccgccgcgg ctgtgttgcc gccgtgacta tctcgtgcct actaccgtcc ggtaattcga
     1501 tccttgtcgc gggcaaggtg ctggcgcaat ccgctccatc gcttcaggcg cacgcgatgg
     1561 agtcgcagag gcgacgatgt gtgatccgag aacgcttgcg tatatgatcg gcgaccgcaa
     1621 cattgagggg tgaccgcggg agggcggggt ggaacagcac gaacaaggcg gccgaccggg
     1681 ttcccgcggg tttggactgt tcgtcggttc tcggttgcgg gaagaacggc ggcttgccgg
     1741 cgcgacgcgg gagacgctgg gcgaggcgct cggcgtcagc agcgagatca tcgagcagtg
     1801 tgaaagcagc ggcaagctgt tgccggcgca tcacctcgcc accgcggctg cggttctgcg
     1861 cgtctctgtg tccgcgctct tctacaatgg cgcggagcgt ggagcgcccg ctgcaaagag
     1921 gcgcgaatac cagcgatggc tcgcgatcag ccggccgccg aagttcctgt atcggcccga
     1981 cttcgccggg ctcgcgccgc ttttggcctt ctggcaggaa agacgcggcg agttcagcgc
     2041 ggaggtcgac agcgccctgt cggccggcgg cattctggaa cgcatgatcc tggtgcggcg
     2101 atcgcctgat tcttcacggc tcgagacaga gcatttcggg gcgaagttca tgttcctgcg
     2161 tccctcggac atcgtcgggc gtgagtttca ggcgcagccg gaccaggaat acgccgcctg
     2221 gatgatcgag gcctatgagc aattgctcga tcgcccggag ctggagctgc gggtcgaagc
     2281 ctgtcgcgcc atcattcacg cctgggacaa tacgaccatc tgtgcccgct atgatcgcgt
     2341 gctcattccc tggcgtcgac gcggttcgga tcaattcttg atgtgcatcg cgctccgccg
     2401 cggcgagccg gcggcagtgc cctcggacac ggacgcgctc tctccgtgac ctcggggccg
     2461 cgctcggccg agtccgcggc gcggtttcgc cgcggctggc gggagcgatt cgattggcat
     2521 gcgcggcgcg gcggcgcggc cggcatcatt gccgtgctca ccgccacgca atggctgaac
     2581 tggagctcga cctattatct gatgacggtg ctcgcccgac ccattgccgc cgaaaccggg
     2641 tggtccttgg cgttcgtggt gaccggcctg tcgctcgggc tcgtgacggc cggattggtc
     2701 tcgccgcgag tcggacgcgc catcgagcgc catggcggac ggcctatgct cgcgctcgga
     2761 tccctgctgc tggcggttgg gttgctgggt gtcgggctgt cgcgtcaccc catcggatac
     2821 ctggcagcct ggatcgtcat cggcgtcgct atggcggcgt ccctgagcga tgccgccttc
     2881 gcgacactgg gcaggtttta tggtctgcgc gcgaagacga tcatcagcaa cctgatggtg
     2941 ctgatcgcct tcagcgccac tttctcttgg ccgttcaccg cgttgctgac ggaagcgctg
     3001 ggctggcgtg ggacgtgctt ctgctacgcg gccatgcatc tcgcgatcgg gctgcccatg
     3061 catttgttcc tgctaccgaa gacagcgaca ttgcccgtcg agctctcgcc atcggtcccg
     3121 gtgcgcgcag aaacggcttc ctttcaggcg gtgcagggcg ccgggccgga tcggcgcacc
     3181 ctgctggtct ggctggtcgg gacgaacatt acactccaga tcgcgatcgg ctcggttctc
     3241 gcggttcatt tgctctcgct tctggagact ctcggcgtcg cctatgcggc ggccgtcgga
     3301 ttcggcagcg tgatctggct gtcgcaggcc ggcggacgcg tgatcgacgc ccttttcggc
     3361 cggcgctttc atccggtgct ggaaggcgtg gttgcaagcc tgctggtgct gctcggcatc
     3421 gctctgctcc tcgccgcgcc gccctcggcc ctgctcgtcg gcatcgtgct gttcggcgtc
     3481 ggaaacggcg tgagaggaat tctcaaagga acgctgccgc tggttctgtt cggcgccgaa
     3541 ggctatgcga cgctgatcgg gcggctcggc cttccgacgc tgatcgccca ggccgcggga
     3601 ccggcgctgg gcgctgtcgc tcttgcgcaa tggggcgcca tgcccttgct ggccatcctc
     3661 gtcgcgcttg cggcgctcaa cctcggcctt gccgccgcgt tgtggatggc aacgccgcgg
     3721 ctcgcgcgct gaaggccggc gcgaccccct actcgctgca ggcgacgtgc gtgtactcgc
     3781 cggtgctggg gtcctcgtag gagagcgctg ccgccatcac cgtacagacg ctgcgggagg
     3841 tctcggtcga gaaggcgcgg ggcgtctcca tctcgctcgt acggaaatgc actttcatgc
     3901 gcgcggcact gtcccaaagc atcgacgcga acggcatcgc cagagccgcg aacacgaaga
     3961 ttccggcagc cacgtcgcga accgtagagc gcttgccctg catgaagcac ctccgcaaga
     4021 ccaccagaca cgctcttcaa ggcgaagcgc gttgcccggc ttcgtggcaa atcacctgct
     4081 ttgatctacg caagttccgt gccgttcatc cccgcgctcg aacgagcgca gccgctcagg
     4141 ccgttccgat gcgatgcgcg atcttgaggg cgggcggctt ggcaagggtc tgaaatacca
     4201 cgcaatagcg ttcggtgagc cgcaggagcg tggcgatctc ctccggggtt gcagggcaat
     4261 cgatgtcgaa gctgagacgg atctcgcgga agccgaccgg cgcgtccttt gcgacgccga
     4321 gcgtgccgcg aaaatcgaga tcgccctcgg cgctcacggc gcctcctttg agcggcaggc
     4381 cgagcgccgt cgccaccgcc ttcatggtga cgcccgcgca agcgacgagc gcttcgagaa
     4441 gcatgtcgcc cgagcagagg ctgagcccgt cgccgccggt ggccgggtgg aggcccgctt
     4501 cggcgagagc gcggccggtc tcgaccttgc aagtgatgcc ttcctcgccg agcctgcctt
     4561 tcgccttgag cgtgatcacg gccttctccg gcgcttcgcg atagagcgcc ttgaacggcg
     4621 cctgcatctc gcgcaaatcc tcggccttca tcatcccgtc cctttcccga tcgttaacct
     4681 tggcgcggcg gtgccttcct tgcccctcgc ttcgcttcca gcgagggcct agaatgcggc
     4741 cgcctgctca ttccggacga tgccaccatg caagaagagt tctatcgcat caagagactg
     4801 ccgccctatg tcttcgccga ggtcaacgcg ctcaaggcca aggcgcgagc ggccggtcgc
     4861 gatgtgatcg atctcggcat gggcaatccc gacggcgcca cgcccgagca catcgtcaag
     4921 aagctcctcg aagccgtgca gaacccgaag agccatggct attcggtgtc gcgcggcatc
     4981 accgggttgc gccgcgctta cgccgcctat tacgcgcgcc gcttcgacgt gacgctcgat
     5041 cccgagcgcg aggtcatcac cacgctgggc tcgaaggagg ggctcgctaa tctggcgcag
     5101 gcgatcactg cgccgggcga catcgtgctg gtgcccaatc cctgctatcc cattcacgct
     5161 ttcggcttca tcatggcggg cgcgtcgctg cgccatgtgc cgatcggacc gagcgtcgac
     5221 ttcatgggcg agctcaagaa agcggtgatg cacagcgtgc cgccgccgat cgctatcgtg
     5281 ctcaacttcc cggccaatcc gacggcgcag gtggtggggc tcgacttcta tgccgaagtc
     5341 gtcgacttct gtcgtcacca caacatcttc gtgctgtcgg acgtggccta tgccgagatc
     5401 tatttcgacg gcaacccgcc gccatcggtg ctgcaggtac ccggcgcgcg cgacatcgcg
     5461 gtcgagttct cctcgctcag caagacctac tcgatggccg gctggcgcat cggcttcgct
     5521 gtgggcaatg cgcgactgat cggcgcattg gcgcgcgtca agtcgtacct cgattacggt
     5581 gccttcacgc cgatccaggt ggcggcgacc gccgcgctca acgggccgca ggactgcatc
     5641 gccgagatcc gcgaacgcta caaggcaagg cgcgacgtcc tcatcgaagg cttgcgccag
     5701 gcgggatggg aagtcccgag cccgcccgcc agcatgttcg cctgggcgcc gcttccgccg
     5761 cgcttccagg agatgggcag cctcgccttc tcgaagctgt tgctcgagca agccaatgtc
     5821 gcggtctctc ccggcgtcgg cttcggcgag tatggcgaag gctatgttcg catcgcgctc
     5881 gtggagaacc gccagcggct gcgtcaggcc acgcgcaaca tcaagcagtt cctgagcggc
     5941 cgcaacagcg ccgccggcca gacccgcgcg caaccgatcg ctgcgacccc cgcctgaccg
     6001 gaattgccgc ctcctttcgg tggacggacg gctgtgggtg ttcgacacgt acggccaggc
     6061 ggcctcccgg gggctctttc caggcgtttc aatgccataa gcggcacaaa tgatgcttga
     6121 ccgcagcttg cggccgcgtc agcctgccta acgatgcggc accgcccgcg cggcacagtc
     6181 agaaccatcg atcgggacgg cgctccggca gccgagtcgt ccccgccggg ccgggttgtt
     6241 tccggtcgca gagcggatac cggaaagacc atcgatctcg cagaccttcc cccggccgaa
     6301 gtcgtcgcag cgcatctggc gccgcgcgag ccggtgccgc cgctcgatgc cgtcacgatc
     6361 cgccaatggc tctggggccg cggcttctac ctccccggcg atgcgcgcca tgtgctggag
     6421 ctcgttcggc cgcttgcgct cgcgccggcg atgacgctgc tcgacgtcgc tgcgggcctg
     6481 ggcgggcccg cctgggcgat cgccgatgaa ttcggcacga ccgtgagcgc gctcgagcgc
     6541 gaccacgagc tcacccggcg cggcgagacg atcgccagcg gcatggcgaa gccggcaggc
     6601 gtgaaggtct tcgatcccga gaggttcgag ctgcgcggcg gcgcgttcga ccgcgtgctt
     6661 gcgcgagaag cgacctatat ggtcgacgac aaagagcgct tcctgcgcgt gctctcgctt
     6721 gcgctgaggc ccgacgggcg gcttctcatg accgatttcg tgatcgagcc gtcgaatgcc
     6781 gagcagccgg cgcaggccgc ctgggcggcg ctccagccgc atcgcccgcg gctctggggc
     6841 ttgcgccaat acagcgattg cctgcgcggc ctcggtctcg atctccgggc gcccgaggac
     6901 atgacggcgg cctacaaggc gcagatcctg cgcggctggg acaatctcct tcagaccgtc
     6961 gatctccgct cgctgccgcg cgcccatcgc accgccgtcg tcgacgaggc cgagcgctgg
     7021 atgcgcacgg tcgccgccct cgacgcgggc accctcaaat tctatcgctt caccgccctt
     7081 gccgggtccg cgctgcggcg ctaacgcgcg ttgatttccc cacgccccgt gtgggagagg
     7141 gcattgacgt tgacagagtc gcccctgttc cctatggtgc gcggccttcg tccagggatc
     7201 cgccatgaaa atcgccaatt cgttgaagtc actgaagaag cgcgacaaga actgccgcat
     7261 cgtgcgccgc aagggacggg tctatgtcat caacaagacc aaccctcgct tcaaggcgcg
     7321 ccaaggctga tccccggccg ttccgagggg tgtcaaaccg cgccgtgccg gcgcggtttt
     7381 tcgttggccg ctcttgttgt tcggcgaacg cggccctgct tagatagcgc cgggagcctc
     7441 tggccatgcg catgccggtg cccgatgccg cgctgatcgc ccgccgcgcc gagatcgcgg
     7501 ccgcgctggc gcggatggtg ccgcccgagg cggtgatcca gaacgaggat gagcggcgcg
     7561 tctacgagag cgatgggctc accgcctatc gccagccgcc gctgcttgcg gtgctgccgt
     7621 ccacgacgga gcaggtcgcg cgcatcctcc gttattgccg cgagaacgcc gtcaaggtgg
     7681 tgccgagggg cgccggcacg tcgctgtcgg gtggcgcact gccgctcgcc gacggcatca
     7741 tcctcggcct gggcaagttc aaccgcatcc tcgacatcga tttcgccaat cgctgcgtcg
     7801 tggcgcagcc cggcgttacc aacctcgccg tgacgacggc cgtgcagcat cagggattct
     7861 tttacgcgcc cgatccgtcg agccagatcg cctgcaccat cggcggcaac gtcgccgaga
     7921 actcgggcgg cgttcattgc ctcaaatacg gcctcaccac caacaacctg ctcggcatcg
     7981 agatggtgct gatgagtggc gaagtgctgc ggctcggcgg caagcacatg gaggccgagg
     8041 gctacgatct cctcggcgtc atgaccggat cggagggact gctcggcgtc gtcaccgaag
     8101 tgacgctgcg catcctgcgc aagccggcga cggcgcgcgc catcctcatc ggctttccct
     8161 ccaacgagag cgcgggcaac tgcgtcgccg ccatcatcgc cgccggcatc attccgggcg
     8221 gcatggagat gatggacaag cccgccattc acgccgccga ggacttcgtg catgtgggct
     8281 acccgctcga cgtcgaagcg ttgctcatcg tcgagctcga cggacccgcg gtcgagtgcg
     8341 atcacctgat gggacgggtc gaagccattg cgcgcgagca aggggcggtg tcgctgcgtg
     8401 tctcgaacag cgaggcggag cggctcgcct tctgggccgg gcgcaaagcg gcctttcccg
     8461 ccgtcgggcg catctcgccc gactattact gcgtcgacgg cacgattccg cgcaagcgcc
     8521 tgcccgacgt gctgacgcgc atgcgcgagc tctcgcagaa gcacggcctt cgcgtcgcca
     8581 atgtcttcca tgccggcgac ggcaacctgc atccgctcat cctctacgac gccaacaacg
     8641 agggcgagct cgagcgcgcc gaggctttcg gcgcggacat cctgcgcttc tgcgtcgagg
     8701 tcggcggcgt tctcacgggc gaacacggcg tcggcgtcga gaagcgcgat ctcatgccgg
     8761 tgatgttcga cgaggtcgac ctgaaccagc agcagcgcct caaatgcgcc ttcgaccccg
     8821 acggactgct caatccgggg aaggtattcc ctgagcttca tcgctgcgcc gaactggggc
     8881 gggtccacat ccaccgcggc gcgacgcggt tcccggacct gccgcgattc tgaggcatgg
     8941 cgtgacccgc tttcgcccga gcgaccagcg cgcgctgacc gagatcgtcc aggcggcggt
     9001 agcggaggag gaaccgctgg aggtgatcgc cggcggcagt aagcgcggcc tcggccggcc
     9061 gatccaagtg ccgcatatcc tcgacgtctc ggccttttcg ggcatcagcg cctacgagcc
     9121 cgaagagctg gtgctcaccg cgggcgcagc cacgccgctc gccgagatcg agcgcatgct
     9181 cgcggccgcc gggcagatgc tggccttcga gccacccgat tggcgcgggc tcctgggcag
     9241 cgacgccgcc gcgccgacgc ttggcggcac gatcgccgcc aaccttgccg gaccgcggcg
     9301 ggtccgccaa ggcgccgcgc gcgaccactt cctgggcttt cacggggtga gcgggcgggg
     9361 agaggcgttc aaggcgggcg gccgtgtcgt caagaacgtc accggctatg acctctgcaa
     9421 gctgctggcg ggctcgcacg gcacgctggc ggtgctcacg gaagtgacgg tgaaagtgct
     9481 gccgcgcgcc gaggccgcgc gcagcgtcgc gctccatggg ctcgacgacg cggcggcgct
     9541 ccgggccatg acggtggcgc tgggcagccc gcacgaggtc agcggcgcgg cccatctgcc
     9601 gaagggcgag aatggggcgg gcgcgccgct cacgctgctc cggctcgaag ggccggagcc
     9661 atcggtcaag gcgcgcgccg cggcgctacg gcaggagctc gccggtgccg gggcggtcga
     9721 ggagctgagc gatgccgaat cggcgcggct gtggatggcg gtccgcgacg tcgcgcccct
     9781 cgtggcgccg agggatcgcg cggtctggcg tatatcggtg ccaccgagcg cgggtccggc
     9841 gctcgccgcc ggcgtggcgc gcgccgtcga tctggactat gtcttcgact ggggcggcgg
     9901 cctcgtctgg ctggccgtga aaggcgcgga agatgggggt gcagcggaga tccgtcggcg
     9961 cgtcgcccag agtggcggcc atgccaccct cattcgcgcg cccgatgcgc tgcgccttgc
    10021 ggtgccggtg ttccagccgc tgccatcggc gctggcggcg ctcacggcgc gcgtcaagga
    10081 cggcttcgac cccaagcgca tcttgaatcg cggccgcatg tattcggagc tgtgatgcgt
    10141 gtttgtatcc ggcaagcatt tcaatcccct ctcccgcttg cgggagaggg ccagggtgag
    10201 ggggcggtga agttggcacc gccgccgcag atcctgcacc gctccctcac cccgaccctc
    10261 tcccgcaagc gggagaggga gaagaaaaat tcgcggacgt aaatcatgca gacgcatttc
    10321 tcgctgacgc aactggccga tcccgatctt cgcgaggccg acaagattct ccgcgcttgc
    10381 gtccattgcg gcttctgcac ggcgac
//
LOCUS       DASYNS010000322         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_235051, whole genome shotgun sequence.
ACCESSION   DASYNS010000322 DASYNS010000000
VERSION     DASYNS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235051"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /locus_tag="VGP50_09985"
     CDS             complement(<1..1760)
                     /locus_tag="VGP50_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8027751.1"
                     /translation="MTEISRAALFGKLNSLGYRAIESATVFCKMRGNPYVELVHWLHQ
                     ILQQQDSDIHRIVKYFNLDAARLAKDVVDALDRLPRGATSISDLSSHVEDAVERGWVF
                     GTLMFGDSQVRTGHLIVGMMKTSTLRNALLAISREFAKIKPEALTEDFDRIVAGSPEE
                     ALRPTDGSVVGGGAVPGEASGAMAPAQMGKQDALKRFSVDLTEKARKGEIDPIVGRDQ
                     EIRQIVDILMRRRQNNPILTGEAGVGKTAVVEGFALRIAAGDVPPPLRDVSLRTLDVG
                     LLQAGASMKGEFEQRLRQVIDEVQASPKPIILFIDEAHTLIGAGGAAGTGDAANLLKP
                     ALARGTLRTVAATTWAEYKKYFEKDPALTRRFQVIQVDEPSEEKAILMMRGMVSTMEK
                     HHRVQVMDEALEASVKLSHRYIPARQLPDKAVSLLDTACARVAISQHAVPAEVDDSRR
                     RISALDTELEIIGREKAVGVETAQREGQANEKLTAEHERLKALEARWQEEKALVDRIL
                     EMRAKLRGSSGTVEGTSSALEKGAAVAKTGEPDPAAAAAPLTGLDRERLLSKLYDAQA
                     QLAELQGETPLILPSVDAQAV"
     gene            complement(1827..>2085)
                     /locus_tag="VGP50_09990"
     CDS             complement(1827..>2085)
                     /locus_tag="VGP50_09990"
                     /inference="COORDINATES: protein motif:HMM:NF018675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEV8027752.1"
                     /translation="FGPLSLAQYLRLLPGGESLRRLVPLVRSYVGDELDWDVNLILKR
                     EEIPPLELGRQGRLGWTTWLTAPLRTGDAGDLRLNPLAAAR"
BASE COUNT          316 a          694 c          703 g          372 t
ORIGIN      
        1 acggcctggg catcgacgct cggcaggatg agcggtgtct cgccctggag ctcggcgagc
       61 tgcgcctgcg cgtcgtagag cttggagagc aggcgctcgc gatcaaggcc ggtgagcggg
      121 gccgcggccg cggcgggatc gggctcgccg gtcttggcga ccgcggctcc cttctcgagg
      181 gcgctgctcg taccctcgac ggtgccggag ctgccgcgca gcttggcgcg catctcgagg
      241 atgcggtcga ccagcgcttt ctcttcctgc cagcgtgctt cgagagcctt gagccgctca
      301 tgctcggcgg tgagcttctc attggcctgc ccctcgcgct gcgccgtttc gacgccgacg
      361 gccttctcgc ggccgatgat ctcgagctcg gtgtcgagcg cggagatccg ccgccggctg
      421 tcgtcgactt cggccggaac cgcatgctgg ctgatggcga cgcgggcaca cgccgtgtcg
      481 agcaggctca ccgccttgtc cggcagctgc cgcgccggga tgtagcgatg cgacagcttg
      541 accgaggcct cgagggcctc gtccatgacc tggacgcgat gatgcttctc catggtcgac
      601 accatgccgc gcatcatgag gatcgccttc tcttccgaag gctcgtccac ctggatgacc
      661 tggaagcggc gggtgagcgc cggatctttc tcgaaatact tcttatattc ggcccaggtg
      721 gtggcggcga cggtgcgcag cgttccgcgc gccagcgccg gcttcagcag attggcggca
      781 tcgccggtgc cggcagcgcc gcccgcgccg atcagcgtat gtgcctcgtc gatgaagagg
      841 atgatgggct tgggcgacgc ttgcacctcg tcgatcacct gacgaagccg ctgctcgaac
      901 tcgcccttca tgctggcgcc ggcctggagc aggccgacat cgagcgtgcg caggctcaca
      961 tcgcgtaatg gcggcggcac gtcgcccgcg gcaatgcgca gcgcgaagcc ctcgaccacc
     1021 gctgtcttgc cgacgccggc ttcaccggtg aggattgggt tgttctgccg gcgccgcatg
     1081 aggatatcga cgatctggcg aatttcctgg tcgcgtccga cgatcggatc gatctcgccc
     1141 ttccgcgcct tctcggtgag gtcgacggag aagcgcttca gcgcgtcctg cttgcccatt
     1201 tgcgccggcg ccatggcgcc gctggcttcg cccggcaccg cgccgccgcc gacaacggag
     1261 ccgtcggtcg ggcggagcgc ttcctcgggc gagccggcga cgattcggtc gaagtcttcg
     1321 gtaagcgcct ccggcttgat cttggcgaat tcgcgcgaga tggcgaggag cgcgttgcgc
     1381 aaggtcgagg tcttcatcat gccgacgatc agatgcccgg tccgcacctg ggaatcgccg
     1441 aacatcaagg tgccgaagac ccagccgcgc tctaccgcat cttcgacatg cgaggagaga
     1501 tccgagatcg aggtggcgcc ccgcggcagc cggtcgagcg cgtcgaccac atccttcgcc
     1561 agccgcgcgg catcgagatt gaaatacttg acgatgcggt ggatatcgga gtcctgctgc
     1621 tgcaggatct ggtgcagcca atgcaccagc tcgacatagg ggttgccccg catcttgcag
     1681 aagacggtgg cgctctcgat ggcacggtag cccaggctgt tgagcttgcc gaacaaggcg
     1741 gcgcggctga tctcggtcat aacgtactcc tcaactcatg aatggccgga acggatggcc
     1801 ccactctttt gctcgagtca tggcgctcac cttgcggccg cgagcggatt gagccgcagg
     1861 tcgccggcgt cgcccgtgcg cagcggcgcc gtcaaccatg tcgtccagcc gaggcgccct
     1921 tgccgaccga gctcgagcgg cgggatctcc tcgcgcttca agatgagatt cacatcccaa
     1981 tcgagctcgt cgccgacata gctgcgcacc agcggcacga ggcggcgcag gctttcgccc
     2041 ccgggcagca ggcgcagata ttgcgcgagg ctcaaagggc cgaag
//
LOCUS       DASYNS010000323         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3668805, whole genome shotgun sequence.
ACCESSION   DASYNS010000323 DASYNS010000000
VERSION     DASYNS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3668805"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..677)
                     /locus_tag="VGP50_09995"
     CDS             complement(81..677)
                     /locus_tag="VGP50_09995"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEV8027753.1"
                     /translation="MRRCNSRSGRTPRPIDIVAERFDAGIALRERVAADMIAVRVTGP
                     LKLAVVAAPTYLARRRAPRTPDDLARHSCIQFRVAVNGPIVEWPFQIKGRSRRIAVKG
                     PVIVNSIPLILRAAVDGLGIALTAEADAKPFLSSGQLVSVLENWTPSLEGLFLYYSGR
                     RQVPGALRAFIDMVGAAGKPKSGRALKNPFAEGVKGAS"
     gene            1531..1833
                     /locus_tag="VGP50_10000"
     CDS             1531..1833
                     /locus_tag="VGP50_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027754.1"
                     /translation="MIEPGEHRDWLSPISGQQEVLMSTSVAPSRSRLSISEAADLAAA
                     MFATLPRGVAEALADPIVQALMAADRVDPRGLAALMCLIAARLADKTPATGRDFSW"
     gene            1827..>2807
                     /locus_tag="VGP50_10005"
     CDS             1827..>2807
                     /locus_tag="VGP50_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV8027755.1"
                     /translation="MVKFFIERPIFSSAVAIVMVLAGLIAFELLPVAEFPDITPPQVV
                     VSATYPGASAQVVADTVTTPLEQQINGVAGMIYMSSVSADDGSATITISFDVGYPLNI
                     AAVDVQNRVAQAAPQLPAIVNQGGVLVQKKNPSILLAINLYSPDNSVDLVTLSNYAYL
                     QLVDPLKRLPGVADVTIFGERRYSMRVWLDPDKLAQLGITAVDVQNAIAEQNVQVAAG
                     RIGAAPAPPGTQFEVQINALGRLKDPQQFGDIVVRAGAGSSATVRLRDVARIELGALQ
                     YTSSSKLDDKPSIFVAVQQQPGSNALDLDKAVRAKMAALAARFPKGIGYGI"
BASE COUNT          473 a          967 c          899 g          468 t
ORIGIN      
        1 aaggccgctt ctctaccatc gcgcaggttc acagcaggcc tctcggccgg ccggcgcgtg
       61 ctcgctgcgc tgtggtggtt tcagctcgcg cccttgaccc cttccgcgaa cgggttcttg
      121 agcgcgcgcc cggactttgg cttgccggcc gcgccgacca tgtcgatgaa agcgcgcagc
      181 gcgcccggca cctgtcggcg tccggaataa taaaggaaga gcccctcgag ggacggcgtc
      241 cagttctcca gcacgctgac gagttgtccc gaactcagga atggcttggc gtcggcctcg
      301 gcggtaagcg cgatgccgag cccatcgacg gccgcccgca ggatcaaggg gatgctgttc
      361 acgattaccg gccccttgac cgcaatccgt cgcgaccttc ccttgatctg gaacggccat
      421 tcgacgatgg ggccattgac ggcgacgcgg aattgaatgc agctgtgacg cgcgagatca
      481 tcgggcgtgc gcggcgcgcg gcggcgcgcg aggtatgtcg gcgcggcgac caccgccagc
      541 ttcagcggcc ccgtgactcg cacggcgatc atgtcggccg caacccgctc tctgagcgcg
      601 atcccggcat cgaatctttc ggcgacgatg tcgatgggcc tcggggtcct cccagacctc
      661 gagttgcacc tccggatagg tcatcaagaa gcgggcccat accggcgcga tcacggcgcc
      721 ggcagcgatc ggagccacgt tgatccgcaa gcgcccgaac ggccgctgtc gctcctggtc
      781 caaatcccgg agcgctccgc cgatctggtc gatggcgggg cggagccgct cgaggaggcg
      841 caggccggca tcggtcagcg agacgctgcg ggtcgtgcgg ttaagcaggc ggacgccgag
      901 gcgttcctcg agctgtcgga tcgtgtggct gagcgccgag ggcgtgacgt cgagccgcgt
      961 cgccgccgcc cggaagctga gatgttccgc gaccgcgaca aaggctgtca gatcggcgag
     1021 atcgctgcgg cgcattgctt gagtcccctt catccctgag cacgagaata tgggatttcg
     1081 ccaggccgct ctgtcaaatg acgacgcatc gtcctgcggg attagtgaac tccgctcagc
     1141 actgaataga ggcgcgcccc gattttcatt gtcgccgacg atacgcacat attggaggca
     1201 actggttggc cgggacgggg ttgacggcag agcggcagcc tccgagccag gcaagacaaa
     1261 ccgagaaggc atccggcatg acattcccca tccaccccgg ctcgtcctag gccccactca
     1321 tgacgagccg gcggccctgg cgcctgcccc cacggcgcca gggccttccc tctgcctctg
     1381 atcacaagac gcgagaaaga tgtcggcgcc cgcaggagaa agggcgcgat gacggcctcc
     1441 ccggcaaacg actccttgcg atcggccggc aatcgctgca tcggctggct gcgttttttc
     1501 ttgtcctgaa cgctgggcga cgagcccgcg atgattgaac cgggagaaca cagagattgg
     1561 ctttcgccga tttcgggaca acaggaggtc cttatgtcca catccgttgc gccgtcgcgc
     1621 tcacgccttt ccatcagtga ggccgcggat ctcgccgccg cgatgttcgc cactctgccg
     1681 cgcggcgtgg ccgaggccct cgcggatccg atcgttcagg cgctcatggc ggccgaccgt
     1741 gtcgacccgc gcgggctggc ggcgctcatg tgcctgatcg cggcccggct tgccgacaag
     1801 acgcccgcca cggggagaga tttttcatgg tgaagttctt catcgagcgg ccgatcttct
     1861 cgtcggccgt cgccatcgtc atggtgttgg ccgggctcat cgccttcgag ctgctgccgg
     1921 tcgccgagtt tcccgatatc acgccgccgc aggtggtggt gagcgcgaca tatcccggtg
     1981 ccagcgcgca agtcgttgcc gacacggtca cgacgccgct cgagcagcag atcaacggcg
     2041 tcgccggcat gatctacatg tcctcggtca gcgccgatga cggcagcgcc accatcacca
     2101 tcagcttcga tgtcggctat ccgctcaaca tcgccgcggt cgacgtgcag aaccgggtcg
     2161 ctcaggcggc gccacagctc ccggcgatcg tcaatcaggg cggggtcctc gtccagaaga
     2221 aaaatccgag catcctgctg gcgatcaatc tctattcgcc ggacaactcg gtcgacctcg
     2281 tcacgctcag caactacgcc tatctccagc tcgtcgatcc cttgaagcgc ctccccggcg
     2341 tcgccgacgt caccatcttc ggcgagcggc gctactcgat gcgcgtctgg ctggatcccg
     2401 acaagctggc tcagctcggc atcaccgccg tcgacgtgca gaacgccatc gccgagcaga
     2461 acgtccaggt cgccgccggc aggatcggcg cggcgccggc gccgcccggc acccaattcg
     2521 aagtccagat caacgcgctg gggcgactca aggacccgca gcaattcggc gacatcgtgg
     2581 tgcgcgccgg ggcgggcagc agcgccacgg tgcggctccg cgacgtcgcc cgcatcgagc
     2641 tgggcgcgct ccaatacacg tcgagcagca agctcgacga caagcccagc atcttcgtcg
     2701 cggtccagca gcagcccgga tcgaacgcgc tcgatctcga caaagccgtc cgcgccaaga
     2761 tggcggcgct cgccgcgcgt ttccccaagg gcatcggcta cggcatc
//
LOCUS       DASYNS010000324         6076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1950416, whole genome shotgun sequence.
ACCESSION   DASYNS010000324 DASYNS010000000
VERSION     DASYNS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950416"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VGP50_10010"
     CDS             <1..1075
                     /locus_tag="VGP50_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8027756.1"
                     /translation="MQDPKLIIMDEPTSVLTPQEAELLFETLARLAGEGRSILYITHR
                     LEEVGRLCDRATVLRHGRVVATVAPREETAASLARHMVGTEVKPVEHRPSRAAAPLLE
                     LRSLSLPAHDIMGVPLSEMSLTVRAGEVVGIAGVAGNGQSELFAALSGERLSRRDAVA
                     IAGRPVGDRGINQRRRLGAAFVPEERLGHGAVPRLSLSANVMLSRHATGALTRFGVVL
                     QKRARQMADQISAAFDVRKSGRDPEAGSLSGGNLQKFLVGREILARPQVLVVDQPSWG
                     VDVRAAVTIRQALIDLAAQGSAVLVISQDLDEILEIADRIAVISHGRLSEAQPTRSLS
                     RERIGLLMGGAHPERARETVDAA"
     gene            1065..2153
                     /locus_tag="VGP50_10015"
     CDS             1065..2153
                     /locus_tag="VGP50_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8027757.1"
                     /translation="MPLKLERRATPSRALLLTSPLIAVALTVATAALLLAVEGVDPWR
                     GIVIYFVQPLESGWSLQELAVKASPLVLIAVGLSVCFIANAWNIGAEGQYIAGAIFGG
                     WVALTFQDTGGWWVLPLMLLLGAVGGMAYALIPALLRVYFGASEILTSLMLVYVAQLG
                     LDYLVRGPWRDPHGYNFPQTVSFEPAATLPLLFEGGRLHLGVVLAVVAVILGAVLLGA
                     TLRGFELRVVGMAPRAARFAGFEYRRDILLAFAISGALAGLAGIIEAAGPIGQLLPTI
                     SPGYGFAAIIVAFLGRLNPVGILLAGLALALSYLGGEAAQIALHVPLDFAQVLQGVLL
                     FYVLACDTLIAYRVRFVASRQGMAYRAS"
     gene            2140..3054
                     /locus_tag="VGP50_10020"
     CDS             2140..3054
                     /locus_tag="VGP50_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8027758.1"
                     /translation="MVLLEAILLTVITASTPLLIAALGELVVERAGVLNLGVEGMMVM
                     GASCGFAVAVLSGSTTLGACAGIAAGMALAALFALMTVGLAANQVASGLALTILGLGL
                     SGLIGADFVGARRDPAPKLHLDGLSDVPFLGPILFGQDAFVYLSVALLLGVSWFLWRT
                     RGGLVLTAIGDNPGSAHALGHKVRFVRLGAILFGGACSGLAGAYLSLAYTPFWVRGMT
                     AGRGWIALALVVFSSWIPWRLAVGAYLFGAVTILQLHVQGFGLGISPQLMSSLPYLAT
                     ILVLVAISRSRRRGASPASLGIPFVPDR"
     gene            3147..4220
                     /locus_tag="VGP50_10025"
     CDS             3147..4220
                     /locus_tag="VGP50_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV8027759.1"
                     /translation="MRRDFLKGAAAAASLPLVSTGAGAAQKLKAGFIYVGPIGDFGWS
                     YQHDVARKALAKALGDSVETSFVENVPEGPDAERAIEGLVRDGAGIVFATSFGFMDAT
                     VKVAKKYPDVKFEHATGYKRAANLATYSGRFYEGRYVIGQIAGKMSKKGVVGYVASFP
                     IPEVVSGINAFMLGAWSVRPDMKVKIVWVNSWFDPGKEADAAKALIDQGADIITQHTD
                     SPAAIETAEQRGVHCFGQDSDMIKFGPKSQLSAIVDNWSDYYVARAKAVIDGSWKSGD
                     VWGGLGSKVVVMAPFTNMPGEVASTAEATTAAIASGKLHPFHGPIYKQDGTLAVKEGA
                     VMPDPEILGMNWYVKGIDEKPPG"
     gene            complement(4280..5287)
                     /locus_tag="VGP50_10030"
     CDS             complement(4280..5287)
                     /locus_tag="VGP50_10030"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEV8027760.1"
                     /translation="MMDLASFVRGLPKAELHLHLEGTLEPAMLFALAKRNRIPLAARS
                     PEELAAAYGFADLQAFLDLYYLGCRVLVEPQDFHDLAAAYFARCRSENILHTEVFFDP
                     QTHTARGIPIQHVFEGFFAAAQEAERAFGLSVGFIMCFLRHLDAAEGFKLLDEAAPYF
                     DKLVAVGLDSSERDHPPAKYAGVFAEARRRGLRTVAHAGEEGPASYIWDALRFLDVAR
                     IDHGVRAIEDPELLDHLAREQVPLTVCPLSNIKLRVFSSMADHSFGALYRRGLQVTIN
                     SDDPAYFGGYLSDNYLACAEAFALDPDDLRRIARNSFEASFLSAEEKRRLIERVDAFQ
                     G"
     gene            5404..5874
                     /locus_tag="VGP50_10035"
     CDS             5404..5874
                     /locus_tag="VGP50_10035"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV8027761.1"
                     /translation="MNPDDFMRRAIALSREKMDAGAGGPFGAVIVADGRIIAEGWNQV
                     TSANDPTAHAEIVAIRAACAALHRFSLEGCEIYASCEPCPMCLAAIYWARLDRIYFAN
                     DRNDAARIGFDDDAIYREVALPIAARSIPTARLLPAEGRAAFDAWEQKTDKVRY"
BASE COUNT          920 a         2039 c         2054 g         1063 t
ORIGIN      
        1 gatgcaggat ccgaagctca tcatcatgga cgagccgacc tcggtgctga cgccgcaaga
       61 ggccgagctc ctgttcgaga cgctggcgcg cctcgccggc gaaggccggt cgatcctcta
      121 catcacgcat cggctggagg aggtgggacg gctctgcgat cgcgccacgg tgctccgcca
      181 cggacgcgtc gttgccaccg tcgcgccgcg cgaggagacc gctgccagcc ttgcgcgcca
      241 catggtcggc acggaagtga agccggtcga gcatcgtccg tcgcgtgccg ccgcaccgct
      301 tctcgagctg cggtccctgt cgttgcccgc gcatgacatc atgggcgtac cgttgagcga
      361 gatgtcgctc accgttcgcg ccggcgaagt cgtgggcatc gccggcgttg ccggcaacgg
      421 gcaaagcgag ctctttgccg cgctctccgg cgagcggctc agccggcgcg acgccgtggc
      481 gatcgccggc cggcccgtgg gcgatcgcgg catcaatcag cggcgccgcc tcggcgcggc
      541 gttcgttccg gaagagcggc tggggcacgg ggcggtgccg cgcctcagcc tctcggcgaa
      601 tgtgatgctc tcgcgccacg ccaccggcgc gctgacgcgg ttcggtgtcg tgctgcagaa
      661 gcgcgcgcgg cagatggccg atcagatcag cgccgccttc gacgtgcgca agagcggccg
      721 cgatcccgaa gcgggcagcc tctccggcgg caatctccag aaattcctgg tcggccgcga
      781 gatcctcgcc aggccgcagg tgctggtggt ggatcagccg agctggggcg tcgatgtgcg
      841 cgcggcggtg acgatccgcc aggcgctgat cgaccttgcc gcccagggga gcgccgtgct
      901 cgtgatcagc caggatctcg acgagatcct ggagatcgcc gaccgcattg ccgtgatctc
      961 gcatggccgg ctgtcggaag cccagccgac gcgaagcctc tcgcgcgagc gcatcggcct
     1021 gctcatgggc ggcgcgcatc ccgagcgcgc ccgggagacg gtcgatgccg cttaagctcg
     1081 agcgccgcgc cacgccgtcc cgcgccctgc tcctgacatc gccgctcatc gccgtggcgc
     1141 tgacggtggc gacggcggcg ttgctgctcg ccgtcgaagg cgtcgatccc tggcggggca
     1201 tcgtcatcta tttcgtccag ccgctcgaga gcggatggag cctgcaggag ctggcggtga
     1261 aggcctcgcc gctggttctg atcgccgtgg gcctctcggt ctgcttcatc gccaatgcct
     1321 ggaacatcgg cgcggagggg cagtacatcg cgggcgcgat cttcggcggc tgggtcgcgc
     1381 tgacgtttca ggacacgggc ggctggtggg tcctgccgct catgctgctg ctgggcgcgg
     1441 tcggcggcat ggcttatgcg ctcattccgg cgctgctccg ggtttacttc ggcgcgagcg
     1501 agatcctgac cagcctcatg ctggtctatg tcgcccagct cggcctcgac tatctcgtgc
     1561 gcgggccctg gcgcgatccg catggctaca acttcccgca gaccgtgagc ttcgagccgg
     1621 cggcgaccct gccgctgctg ttcgagggcg gccggttgca tctcggcgtg gtgctggcgg
     1681 tggtcgcggt catcctcggc gccgttctgc tcggcgcgac cctgcgcggc ttcgagctgc
     1741 gcgtcgtcgg catggcgccg cgcgccgcgc gctttgccgg cttcgagtat cgccgcgaca
     1801 tcctcctcgc cttcgcgata tcgggcgcgc tcgccggtct cgccggcatc atcgaggccg
     1861 ccgggccgat cggccagctc ctgccgacga tctcgccggg ctatggcttc gccgcgatca
     1921 tcgtcgcctt tctcggccgg ctcaatccgg tcggcatcct gctggccggg cttgccctcg
     1981 ccttgtccta tctcggcggc gaggccgcgc agatcgcgtt gcatgtgccg ctcgacttcg
     2041 cccaggtcct ccagggcgtg ctgctcttct acgtgctcgc ctgcgacacg ctcatcgcct
     2101 atcgcgtccg cttcgtcgca tctcggcagg ggatggcgta tcgtgcttct tgaagcgatc
     2161 ctgctcaccg tcatcacggc gtcgacgccg ctcctcatcg cggcgctggg cgaactcgtc
     2221 gtcgagcgcg ccggcgtgct caatctcggc gttgaaggga tgatggtgat gggcgcgtcc
     2281 tgcggtttcg ccgtggcggt cctgagcggc agcacgacgc tcggtgcctg cgccgggatc
     2341 gccgccggca tggcgctagc ggcgctcttc gcgctcatga cggtgggcct cgccgccaat
     2401 caggtcgcct ctggcctcgc gctcaccatc ctcggcctcg gcctctccgg gctcatcggc
     2461 gcggatttcg tcggcgcgcg ccgcgacccg gcgcccaagc ttcatctcga cggcctttcg
     2521 gacgtgccct tcctcgggcc gatcctcttc ggccaggatg ccttcgttta tctctcggtg
     2581 gcccttctgc tcggcgtctc ctggtttctc tggcgcacgc gtggcggcct ggtgctgacg
     2641 gcgatcggcg acaatcccgg ctcggcgcat gcgctcggcc acaaggtgcg gttcgtccgg
     2701 ttgggggcga tcctcttcgg cggtgcctgc tcgggcctcg ccggggccta tctgtcgctg
     2761 gcctatacgc ccttctgggt gcgcggcatg accgccgggc gcggctggat cgcgctggcg
     2821 ctcgtcgtgt tctcgtcctg gattccctgg cgcctcgcgg tcggcgccta tctcttcggc
     2881 gccgtcacca tcctgcagct gcatgtccag ggattcggcc tcgggatttc gccgcagctc
     2941 atgtcgtcgc tgccttacct cgcgacgatc ctggtgctcg tcgccatctc gcgcagccgg
     3001 cgccgcggcg cgtcaccggc gtcgctgggc ataccctttg ttccggaccg gtagcatgtc
     3061 cctctccgcg gcggcgaccc gcgcggaacg ggaattgcag gggtcgcggc gcgcggtttt
     3121 cacccgaggc acggaggata gacgtgatca gacgcgattt cctgaaaggc gcagcggccg
     3181 ccgccagcct tccgctggta tcgaccggcg ccggagccgc ccagaagctc aaggcaggct
     3241 tcatctatgt cgggccgatc ggcgatttcg gctggagcta tcagcacgac gtcgcccgca
     3301 aggcgctcgc caaggcgctc ggcgatagcg tcgagaccag cttcgtcgag aatgtgccgg
     3361 aggggccgga cgcggagcgc gcgatcgagg gcctcgtacg cgacggggcg ggcatcgtct
     3421 tcgccacctc cttcggtttc atggacgcga cggtgaaggt cgcgaagaaa tatcccgacg
     3481 taaagttcga gcatgcgacc ggctacaagc gcgccgccaa cctcgcgacc tattccgggc
     3541 gtttctacga gggccgctac gtcatcggcc agatcgccgg caagatgtcg aaaaagggcg
     3601 tcgtcggcta tgtcgcgtcc ttccccatcc ccgaggtcgt gtcggggata aacgccttca
     3661 tgctcggcgc ctggtcggtg cggcccgaca tgaaagtgaa gatcgtctgg gtcaattcct
     3721 ggttcgatcc gggcaaggaa gcggatgccg ccaaggcgct catcgaccag ggcgccgaca
     3781 tcatcaccca gcataccgac agcccagccg ccatcgagac cgcggagcag cgcggcgtcc
     3841 attgcttcgg ccaggattcc gacatgatca agttcgggcc gaaaagccag ctctcggcca
     3901 tcgtcgacaa ctggtccgac tattatgtcg cgcgcgccaa ggcggtgatc gacggcagct
     3961 ggaaatcggg cgacgtctgg ggcggcctcg gcagcaaggt ggtggtgatg gcgcccttca
     4021 ccaacatgcc cggagaggtc gcgagcacgg ccgaagcgac gacggcggcc atcgcctcgg
     4081 gcaagctcca tcccttccac gggccgatct acaagcagga cgggacgctg gccgtgaaag
     4141 aaggcgccgt gatgcccgat cccgagatcc tcggcatgaa ctggtatgtc aaaggcatcg
     4201 acgagaagcc ccccggctga aaattccctc tcccgcaagg ggagagggat ttatgatccg
     4261 gctcaaacga cgctgctgct cacccctgga acgcgtcgac gcgctcgatc agccggcgct
     4321 tctcttcggc ggagaggaag ctcgcttcga agctgttgcg cgcgatgcgc cggagatcgt
     4381 ccgggtcaag cgcgaacgcc tcggcgcaag cgaggtagtt gtcgctgaga tagccgccga
     4441 aataagcggg gtcgtcgctg ttgatggtga cttggaggcc gcggcgatag agcgccccga
     4501 agctgtggtc cgccatcgag gagaagacac gcagcttgat gttcgacaag gggcagacgg
     4561 tgagtggcac ctgttcgcgc gcgagatgat cgagcagctc cggatcctcg atggcgcgca
     4621 cgccgtgatc gatgcgcgct acgtcgagaa accgcaacgc gtcccagata taggacgccg
     4681 gtccttcctc gccggcatgg gcgacggtgc gcagcccgcg gcgccgcgcc tccgcgaaga
     4741 cgccggcata tttcgccggc ggatggtcgc gctccgagga atcgagtccg acggccacga
     4801 gcttgtcgaa gtagggggcg gcctcgtcca gcagcttgaa accctccgcc gcatcgagat
     4861 ggcgcaggaa gcacatgatg aatccgacgg agaggccgaa ggcgcgctcg gcctcctgtg
     4921 cagcggcgaa gaagccctcg aagacatgct ggatcgggat gccgcgcgcg gtgtgggtct
     4981 gcgggtcgaa gaacacctcg gtatgaagga tgttctcgct gcggcagcgc gcgaaatagg
     5041 cggcggcgag gtcgtggaaa tcctgcggct ccaccagcac ccggcagccg aggtaataga
     5101 ggtcgaggaa tgcctggaga tcggcgaagc cgtacgctgc cgccagctct tcggggctgc
     5161 gggccgcgag cgggatgcgg ttgcgcttcg ccagcgcaaa cagcatggcc ggttccaacg
     5221 tgccttcgag atgcaggtga agctcggcct tcggcagtcc gcgaacgaag ctcgcgagat
     5281 ccatcatggc gcgaccgtcc tctcccatga atgccaggat aacgcggcga tcacccgctg
     5341 ctgctggaaa agtggtagca ggcctgcgca taatccagag cgatgcatcg ggagtacgac
     5401 gtgatgaacc ctgacgattt catgcgccgg gcgatcgcgc tctcgcgcga gaagatggac
     5461 gcgggcgccg ggggaccctt cggcgcggtc attgtcgcgg acggccggat catcgccgaa
     5521 gggtggaacc aggtcacctc ggccaacgac ccgaccgctc atgccgaaat cgtcgccatc
     5581 cgcgccgcct gcgcggcact ccaccgcttc agcctcgagg gctgcgagat ctatgcaagc
     5641 tgcgagcctt gtccgatgtg cctcgcggcc atctactggg cgcgcctcga ccgcatctat
     5701 ttcgccaacg accgcaacga tgcggcgcgg atcggcttcg acgacgacgc catctaccgc
     5761 gaagtcgcac tgccgatcgc ggcgcgcagc atcccgacgg cacgcctgct gccggcggaa
     5821 ggccgcgccg ccttcgatgc gtgggagcaa aagacggaca aggtgcggta ttgagcgaga
     5881 tcttttagcg acgcatccac ggcgatggca tgttgcccga caatcttttc gtatcgcgcg
     5941 actttcggcc gcctcaccca cccctgagac tccgcgatga tcgacgaaaa aacaacagaa
     6001 gcgaaagatc cgaatgaagg attttaccgt aagcgtcatt ctttccccac ggctttgccg
     6061 ggttgagggt tcgggc
//
LOCUS       DASYNS010000325         3373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1641803, whole genome shotgun sequence.
ACCESSION   DASYNS010000325 DASYNS010000000
VERSION     DASYNS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641803"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..>3373)
                     /locus_tag="VGP50_10040"
     CDS             complement(136..>3373)
                     /locus_tag="VGP50_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027762.1"
                     /translation="VAGKVGKVAQDIETRATKATEFLANRIGSVAQEIETRTNVAAAF
                     ASGKIDKSVLEIEARTNSAVEFATGRFGQSAQELELRASSAAETAAGKISKVAQDIEV
                     RAAAAADSAVSKVGKLAQDIETRTSAAADSAVGKVGRLVQDIETRAHAAADGAVGRIG
                     KAAQDIETRTGASADLAVNRIAKLTHEVEARTNAAAENAVVKIDRISHEIGARAGAAV
                     EAATLMIGKASHDIETRAQAAAEFAAGRLGSAIEDMEARAASAGEEVSQRIATVTQHI
                     GTRAAEAGSALAERAYSATHDIEARTTAATEFLATRIGAIAGEIEVKSRTAADLLQAR
                     AIDVAGSLKANTSDASRLLIELSSSTAEALRSASSDVERTLTTVSSGTSAMLKQNAAE
                     VERSLLGVSTEVSKTFVGKADEMSIAMRQNSTELARLLDDKSSGLITAIATKGGQFSM
                     EIERAAESAVKAIDTRAFAFGQTMMDNSTELARIINDASASATSIVNRTIKDLQDSTK
                     SSVAQATMAVSQTLKDLQQTTVGAVEESKQTAVATVSELLETHGMLRSDTTALFERLR
                     DANNLLQEVLGGAQTNLSSIEQVLSARVAEFVSTMGDLLERANSTSGKMDEHVGSFYG
                     LTSKVLGDLGELAVQFDGHGRSLSDAVEMLEKSNKHSMVAVTDRHATIENLVTTLNER
                     TEDLDQRLKRFSGMLDESLHVAEDRARDIARLVAEATSEGARSIAEQHATIRSATEEQ
                     SRRTLDSLRNVYEQVTTDSKGLFQETATEAHQLLAQATDRFSDVMQTMKMMSAEMQRE
                     LEATRQELRRGVLELPEETAESTAQMRRVIVDQMEALAELNRIVARHGRNLDTVSTAA
                     PRPEPARPVAQRAEMSAAPAPLSAAATRTRVEAPSVQPNGQNGATPNGAASPNGAGPG
                     RGGWLSELLTRASRDEDAAPPRTEDRQAFAQPRQEERPARGEDRNDRNVRHTIESLDS
                     LSVDIARMIDHDAAAELWDRYNRGERNVFTRRLYTMQGQKAFEEVRRRYKSDREFRQT
                     VDRYIGEFERLLNEVARDDRGQVVARTYLTSETGKVYTMLAHAAGRFE"
BASE COUNT          429 a         1128 c         1183 g          633 t
ORIGIN      
        1 gatcggccaa cgctcgctgc gcacggtcag ggtgcagatg tcagccattg ccgacgatca
       61 gcaatcagtg cgccgggcat cgacggtcag gaatcaacgg ccactgccat tgtgattgct
      121 gatccctgac cactgtcatt cgaaccgtcc ggcggcatgg gcgagcatcg tatagacctt
      181 gccggtttcg gaggtgaggt aggtacgcgc caccacctgg ccgcggtcgt cgcgcgcaac
      241 ctcgttgagc agacgctcga attcgccgat gtagcgatcg accgtctgcc ggaattcgcg
      301 gtcggacttg tagcgccgcc gcacttcttc gaacgccttc tgtccctgca tcgtgtagag
      361 ccgccgggtg aagacgttgc gctcgccgcg attgtagcgg tcccacagct ctgcagcggc
      421 gtcgtgatcg atcatgcggg cgatgtccac cgacagcgaa tccagcgact cgatggtgtg
      481 gcggacattg cgatcgttgc ggtcctcgcc ccgtgccggg cgctcctcct ggcgcggctg
      541 cgcgaaggcc tggcgatcct cggtgcgcgg cggcgcagcg tcctcgtccc gcgatgcgcg
      601 cgtcagcagc tcggagagcc agccgccgcg gccgggcccg gcgccgttcg gactggcggc
      661 tccattcggc gtcgctccgt tctggccgtt cggctgcacc gagggcgctt cgacccgggt
      721 gcgcgtcgcg gcggccgaca gcggcgccgg cgcggcagac atctcggcgc gctgggccac
      781 cggccgggcc ggctccggcc gcggcgcggc agtgctcacg gtatcgaggt tacggccgtg
      841 gcgggccacg atgcggttga gttcggcgag cgcctccatc tggtcgacga tcacccgccg
      901 catctgcgcg gtgctttcgg cggtctcctc gggcagctcg agcacgccgc ggcgcagctc
      961 ctggcgggtc gcttccaatt cgcgctgcat ctccgccgac atcatcttca tggtctgcat
     1021 cacgtcggag aagcggtcgg tggcctgggc cagcaactgg tgcgcctcgg tcgcggtctc
     1081 ctggaacagg cccttgctgt cggtggtcac ctgctcatag acgttgcgga gcgaatccag
     1141 cgtgcgccgg ctctgctcct cggtggcgga gcggatggtc gcatgctgct cggcgatcga
     1201 gcgggcgcct tccgaggtcg cttcagcgac cagccgggcg atgtcgcggg cgcggtcctc
     1261 ggcgacatgc agggactcgt cgagcatgcc cgagaagcgc ttgaggcgct gatcgaggtc
     1321 ctcggtgcgc tcgttgagcg tcgtgaccag gttctcgatc gtcgcatgcc ggtcggtgac
     1381 cgcgaccatg ctgtgcttgt tgctcttttc gagcatctcg accgcatcgc tgagcgagcg
     1441 gccgtggccg tcgaactgca ccgcgagctc gccgaggtcg cccagcacct tggaggtcag
     1501 gccgtagaac gagccgacat gctcgtccat ctttccggac gtcgaattgg cgcgctccaa
     1561 gaggtcgccc atggtggaga cgaactcggc gacccgcgcc gaaagcacct gctcgatcga
     1621 gctgagattg gtctgggcgc cgcccaggac ctcctgcagg aggttgttgg cgtcgcgcag
     1681 ccgctcgaac aacgcggtcg tatcggaacg cagcatgccg tgggtctcga gaagctcgga
     1741 aaccgtggcg accgcggtct gcttggactc ctcgaccgcg ccgacggtgg tctgctgaag
     1801 gtccttgagc gtctgcgata ccgccatggt cgcctgggcg accgagctct tggtggaatc
     1861 ctgcagatcc ttgatggtgc ggttgacgat ggaggtcgcg ctcgcgctcg catcattgat
     1921 gatgcgggcg agctcggtgc tgttgtccat catcgtctgg ccgaacgcga atgcccgggt
     1981 gtcgatcgcc ttcacggcgc tttccgccgc gcgctcgatt tccatcgaga actggccgcc
     2041 cttggtggcg atcgcggtga tgaggccgct gctcttgtca tcgagcaggc gggcgagctc
     2101 ggtcgaattc tgccgcatcg cgatggacat ctcgtcggcc ttgccgacga aggtcttgct
     2161 cacctcggtg ctcaccccca acaggctgcg ctccacctcg gcggcgttct gcttgagcat
     2221 cgcgctggtg ccgctcgaca ccgtcgtcag ggtgcgctcg acgtcggagg aggcggagcg
     2281 gagcgcctcg gcggtcgaac tggacagctc gatcagcaag cggctcgcgt cggacgtatt
     2341 ggccttgagg ctgcccgcga cgtcgatcgc gcgcgcctgc agcaggtcgg cggcggtgcg
     2401 gctcttgacc tcgatctcgc cggcgatcgc accgatccgg gtggccagga attcggtggc
     2461 cgcggtggtg cgcgcctcga tatcgtgggt ggcgctgtaa gcccgctcgg ccagcgcgct
     2521 gccggcttcc gccgcgcggg tgccgatgtg ctgggtgacg gtggcgatgc gctgcgacac
     2581 ttcctcgccg gcactcgccg cgcgggcttc catgtcctcg atcgcactgc cgagacggcc
     2641 ggcggcgaat tcggccgccg cctgcgcgcg cgtctcgatg tcgtgcgagg ccttgccgat
     2701 catgagggtc gcggcctcca ccgcggcgcc ggcgcgggcg ccgatctcat gggaaatccg
     2761 gtcgatcttg acgaccgcgt tttccgcggc ggcattggtc cgggcctcca cctcgtgcgt
     2821 cagcttggcg atccggttga ccgcgagatc cgccgacgca ccggtgcgcg tctcgatgtc
     2881 ctgggcggcc ttgccgatcc tgccgacggc gccgtcggcg gccgcatggg cgcgcgtttc
     2941 gatgtcctgg acgagcctgc cgaccttgcc gacggcgctg tcggcggcgg cactggtgcg
     3001 cgtctcgatg tcctgggcga gcttgccgac cttgctgacg gcgctgtccg cggcggcggc
     3061 ggcgcgcact tcgatgtcct gggcgacctt ggagatcttg ccggccgcgg tctcggccgc
     3121 ggaactggcg cgcaattcga gttcctgtgc ggattggccg aaccggccgg tcgcgaactc
     3181 caccgcgcta ttggtacgcg cctcgatctc gaggaccgac ttgtcgatct tgccgctggc
     3241 gaacgcggcc gcgacattgg tgcgggtctc gatctcctgg gccacgctgc cgatccggtt
     3301 ggcgaggaac tcggtcgcct tggtggcgcg ggtctcgatg tcctgggcga ctttgccgac
     3361 cttgccggcc acc
//
LOCUS       DASYNS010000326        11087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1796721, whole genome shotgun sequence.
ACCESSION   DASYNS010000326 DASYNS010000000
VERSION     DASYNS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796721"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /gene="gor"
                     /locus_tag="VGP50_10045"
     CDS             <1..1367
                     /gene="gor"
                     /locus_tag="VGP50_10045"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV8027763.1"
                     /translation="DFDMFTIGAGSGGVASSRRAGSYGARVAICENSRVGGTCVIRGC
                     VPKKLLAYGAQFADAFADATGYGWRVAPPVHDWPSLIAAKNVEIARLEGVYHRLLRDS
                     GVMLIEGRGRLVDPHTVEVGGKRYTAANILVATGGHPVLPAIPGIEHAISSNEALELG
                     ALPHHIVIVGGGYIAVEFAGIFRGLGAEVTLAIRGEDLLSGFDDDVRVTLAQELRKRG
                     IEIRARTQIARIDKRGHGCDLTTLHGETLSADLVMYATGRRPNTRGLGLAEAGVTLDE
                     AGAVVVDEWSRSSVPNIYAIGDVTDGLNLTPVAIAEGRALAETLFHDNPIKLDHANVP
                     TAVFSQPPVGAVGLTERAARQSHSPIDIYRARFTPLKNTLSGRPETTMMKLVVERQTD
                     RVLGCHMVGVDGPEIIQGIAIAVKCGATKRQFDQTLGIHPTAAEEFVTMRDKLPMPEA
                     EAAE"
     gene            complement(1687..1902)
                     /locus_tag="VGP50_10050"
     CDS             complement(1687..1902)
                     /locus_tag="VGP50_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027764.1"
                     /translation="MPLHIALYWISFIVFSLIVLSPIIRILKRAGYSEWWSLLAFVPV
                     VNLMGLWWFAYARWPKLAPLHPTAPGA"
     gene            2146..3102
                     /locus_tag="VGP50_10055"
     CDS             2146..3102
                     /locus_tag="VGP50_10055"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV8027765.1"
                     /translation="MTVTIGIALGSGVARGWGHIGVLRVLESEGIVPDIVAGSSIGAL
                     IGAVYVGGHLDDLETWARKLTKVRLSRLFDFDFGQGGIIAGRRVLQILHPSLLETTIE
                     RLRSPFVCVATDLNTGHEVWLREGSLIEALRASYAIPGLFPPVALGGRWLVDGAMVNP
                     VPVSVCRALGARLTIAINLNADVFGPAPTGEDALELEQRAVRTEEKDGGGFMRAYFRR
                     RNGSPSTFSVLARSLHILQDRISRSRLAGDPPDVMIAPRLGHIGYLEFYRAEECIAAG
                     EEAARRAVPEIREAIRQLGTSSAWNRNARLRAGESGDADGSE"
     gene            3187..3732
                     /locus_tag="VGP50_10060"
     CDS             3187..3732
                     /locus_tag="VGP50_10060"
                     /inference="COORDINATES: protein motif:HMM:NF022605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2937 family protein"
                     /protein_id="HEV8027766.1"
                     /translation="MSFIGRWLGNSINIALALVFAIVAMQAPAFTRDYAEALLQVAQE
                     SRRDIEQRKDSARRFYAIPAEGDEQVIAALKPFEPSNAETLALSVDRARRLQAAYDKV
                     TGTNPLLQPIAALSDAVEDEHGYKTAVLRTLFSTYVPHLDFGVAAAAYGLAGLVTGSF
                     VAQLLAALGRRIGGRRLAREG"
     gene            complement(4109..5344)
                     /locus_tag="VGP50_10065"
     CDS             complement(4109..5344)
                     /locus_tag="VGP50_10065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8027767.1"
                     /translation="MSAPARSIEERPRDAAVIALAGMAHYSSHVMQLALAPLFPLLHD
                     HFAVSFADLGLVMGIFYASSGLGQAAAGVLVDRYGARRLLLGGLVLLSVAIGLMGFAT
                     SYRMLLPLALLGGLGNSVFHPADLSILSHRVGVSRLGRAYAVHGICGSLGFATAPVLV
                     ALIAAFAGWRVALTAIGAGGLAVAALLYANRALLDYRRAVPAPAPGAARPHLAYWRVV
                     GSPVVLMGFTYFALTAFAGTGIQTFSITALVSGYGLELPIATLGLTLYLIGSAIGMMA
                     GGFLAERTRQHHRVAMSGIALAAALMLLIAGLPGGAAVPLMMLAGMASGITAPSRDVL
                     IRQAAAGSGMGSVFGFVYSGFDLGSSIAPLLFGALIDRGAPHAVFLAIACSFAAGVPA
                     VMQVAQRRASRAQAPAGAD"
     gene            complement(5544..6866)
                     /locus_tag="VGP50_10070"
     CDS             complement(5544..6866)
                     /locus_tag="VGP50_10070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV8027768.1"
                     /translation="MENAVDCDVLVVGGGPAGSTAAALLARQGKHVVLLEKEFFPRFH
                     IGESLLPLNLPVFDRLGVADDVRRLGVYKPGAQVFSDEHGKTTVFRFGANPHLTVKHA
                     YQVKRADFDKLLLDNSRRCGATVEEGVRVTDMAFDAGRRATVTAVKADGAVAQWRPRF
                     VVDASGRDTLLATRFETKESDKRNNTAALFGHFRDVQRRDGNAEGMIAVHLFPQGWFW
                     VIPLPDGITSVGMVGTQAFFKSRTGDLDAFFTRAVAATPSLAERMATAQPVGPLVSAA
                     NFSYRSTRVTGDGYILIGDAAAFIDPLFSSGVMLAMSSGAFAVEAVAAQLDGKPEAAT
                     LMREYERRVRRGLDAFAWLIYRINTPILRDMLMEEFDLFDTRNGLIAILAGDFYAPRW
                     FLSPLRRVQLAHAVLVLCAKLGFRLHSRDGRIGLRRASGRLSSDIHAG"
     gene            complement(6960..8039)
                     /locus_tag="VGP50_10075"
     CDS             complement(6960..8039)
                     /locus_tag="VGP50_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV8027769.1"
                     /translation="MSEAAPVAIAAAGRAPLLCVENLTVAYRTGNYRVQAVSDVSFDI
                     ARGETLALVGESGCGKSTLGRAVLQLVRPNAGRVLFDGIDLTRERGERLRRLRRRLQL
                     IFQDPIASLNPRRRVGDIVAEPLIIAGVKDAATRERRVRATLAAVGLDADLVMRRRPH
                     EFSGGQCQRISIARALVLEPDLIICDEPVSSLDVSIRAQILNLLEEMKQRYGLTLLFI
                     AHDLAVVKAISDRVAVMYLGKLCEIGPADEVFRRPGHPYTAALRAAIPVPDPEAPLPE
                     RVVTGEPPSPMAPPSGCRFRTRCPMAAPRCAAEEPAMRAIGPGHFVACHFALGEALGE
                     RAPARSSAAAAAVCAANCESRIEQN"
     gene            complement(8036..8911)
                     /locus_tag="VGP50_10080"
     CDS             complement(8036..8911)
                     /locus_tag="VGP50_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8027770.1"
                     /translation="MATLDAALDREVPVAAAERRRPGLGALFWLAVAWIAIVLLLALA
                     ADLLPLASPFKQTLMARRASPSARHLFGTDGLGRDVLSRLIHGARISLAVGFTAPLLG
                     TVVGTVLGVLAGYFRGRFETLAIAATDVLLAFPPLVLALAIIAYLGASVVNLIIVLGI
                     LTIPAVTRVARATTLSLREREFVIAARALGASHARILRREILPNVLVPLGAFFLLLAA
                     VTIIAEGILSYLGLGVPPPAPSWGSMIAEGRDSLAVAPHVAFIPAAAMFLTVISINLI
                     GDTLRASIDPRRSAL"
     gene            complement(8898..9848)
                     /locus_tag="VGP50_10085"
     CDS             complement(8898..9848)
                     /locus_tag="VGP50_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8027771.1"
                     /translation="MLGFVGRRLLYLIPVVLAVTVLTFFIASLLPGDLAVTMLGDQAT
                     PEAVAALHKKLGLDLPLWQQYLHWLGGALSGDFGTSQRTGETVLHAIAGRLPVSLELM
                     VIAELGGLLIGVPLAILCAVRAGSELDRVASAAAFGTLSVPPFLLAILLIFLFAVQLN
                     WLPATGYVPLSEDILGNLRAMALPGLTLALLEWPAIMRILRSDMISTLQENYIAMAKA
                     KGLSQRRILLVHALKPSSLTLVTIVGLNIGRLIGGALLVETIFALPGVGRLLVEAIFL
                     RDFIILQGGVVLVAIGFVVVNFAVDLLYAALDPRIRHGHA"
     gene            complement(9862..>11087)
                     /locus_tag="VGP50_10090"
     CDS             complement(9862..>11087)
                     /locus_tag="VGP50_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027772.1"
                     /translation="FQDGSPFNAQAVAFNFQRMRNPDNHCRCAAYITAINTIEAADDL
                     TVVYHLNAPQPNWAALIAPKTVTTVIHSPKAIEEMKEGYNRHPVGTGAFRLKSWQSGD
                     QIVLERNPDYWDKDHVYLDEVSVRPLTDAQARYASLQSGESDVIWDDNADNIADARKN
                     ANFAVREYAGSGAVVYVFNMRQPPFDDVRVRQAMRMAMDMPAFADAVASGVSKAAKDP
                     YGPGSFVQCKDTGLLPYDVAKAKALLADYGKPVDFKLIVTATPRGRTYGQIFQEFWSA
                     VGAKVSIDQIDQSTLGARVFSHNFQVAPWGIIDSADPDPQMYANFHTGSPVNLAGYSN
                     PDVDRLLEDARSSGDLEKRSDDYCEIARILNKDVPWFWALEIHSFAIAKAHLEGIPQQ
                     RSGVIDLTTAWWDKK"
BASE COUNT         1949 a         3674 c         3834 g         1630 t
ORIGIN      
        1 acgacttcga catgttcacg atcggcgcgg gctcgggcgg cgtcgcgtcg tcgcggcgcg
       61 ccggctccta cggcgcgcgg gtcgccatct gcgagaacag ccgcgtcggc ggcacctgcg
      121 tgatccgcgg ctgcgtgccg aagaaactcc tggcctatgg cgcgcaattc gccgacgcct
      181 tcgccgacgc cacgggatat ggctggcggg tggcgccgcc ggttcacgac tggccgagcc
      241 tgatcgccgc caagaatgtg gagatcgcgc ggctcgaagg cgtctatcac cgccttctcc
      301 gggattcggg cgtgatgctc atcgaaggcc gcggccggct cgtcgatcca cacaccgtcg
      361 aggtcggcgg caagcgctac accgcggcca acatcctcgt cgccacgggc ggccatccgg
      421 tgctgccggc catccccggg atcgagcacg ccatctcctc gaacgaggcg ctcgagctcg
      481 gcgccttgcc gcaccacatc gtcatcgtcg gcggcggcta catcgccgtc gaattcgccg
      541 gcatcttccg cgggctcggc gccgaggtga cgctggcgat tcgcggcgag gatctgctct
      601 cgggcttcga cgacgacgtc cgcgtgacgc tggcgcagga gctgcgcaag cgcggcatcg
      661 agatccgcgc ccgcacccag atcgcccgca tcgacaagcg cggccatggc tgcgatctca
      721 cgactcttca cggcgagacg ctttccgccg atctcgtcat gtacgccacc gggcggcgcc
      781 ccaacacgcg gggcctcggc cttgccgagg cgggcgtgac gctcgacgag gccggcgccg
      841 tcgtcgtcga tgaatggtcg cgcagcagcg tccccaacat ctatgcgatc ggcgacgtga
      901 ccgacggcct caacctgacg ccggtggcga tcgccgaagg ccgcgcgctc gccgagacgc
      961 tgtttcacga caatccgatc aagctcgacc acgccaatgt gccgacggcg gtcttcagcc
     1021 agccgccggt cggcgccgtc ggcctcaccg agcgcgcggc gcgtcagagc cacagcccga
     1081 tcgacatcta tcgcgcgcgc ttcacgccgt tgaagaacac gctctcgggc cgcccggaga
     1141 cgacgatgat gaagctcgtg gtggagcgtc agaccgaccg cgtgctcggc tgccacatgg
     1201 tcggcgtcga cgggcccgag atcatccagg gcattgccat cgccgtcaaa tgcggcgcca
     1261 ccaagcgcca gttcgaccag accctcggca tccacccgac ggcggcggag gagttcgtga
     1321 cgatgcgcga caagctgccg atgccggagg ccgaagcggc ggagtagggt cgttttccgg
     1381 cagccgcacc agaagcagaa gaggaacgcg gaggccgcgg aggttgcgca gaggacggtg
     1441 agcaacagtc atcctcggcg gaagacgcag ataggaaaga ttcaccccgg aggcacggag
     1501 aaaagaagaa tggcgcgcgt cgcgcgccga ccccgtcttc tccgtgtcct ccgtgcctcc
     1561 gtggtgaaac cattatgcga tggcccggtc gtagagggtt acccagggga gctgcgcgag
     1621 caacggtcgt cctccgcgcc ctccgcgaaa cctcggcgcc ctccgcgttc gctaacgaaa
     1681 agaagcttac gcgcccgggg cggtgggatg gagcggcgcg agcttcggcc agcgggcata
     1741 ggcgaaccac cagaggccca tcaggttcac caccggtacg aaggcgagga gggaccacca
     1801 ctcgctgtat ccggcgcgtt tcaggatacg gatgatcggg ctcagcacga tgaggctgaa
     1861 gacgatgaag ctgatccagt agagcgcgat gtggagcggc atgcgtcccc cgttgcctca
     1921 cgcgaccgcg ccggcgccac gcgcgtccag gcgccggccc gcgagggccg tcacccgaac
     1981 gcttggaggc accgacgttg cctatagtct caaagccatc atttacagat gataaagagc
     2041 tctgtggcca ttttgggcta gcgcctgcga ccaatcgcca attcgacgta atcaggggct
     2101 aaggggttgc cggtaaccat tccgcgcggt tagatcggcg gcgagatgac agtcaccatc
     2161 ggcattgcac tcggcagcgg cgtggcgcgc ggctggggac atatcggcgt tcttcgcgtg
     2221 ctcgaaagcg aaggcatcgt tcccgatatc gtcgccggct cgtccatcgg cgccttgatc
     2281 ggcgccgtct atgtgggcgg ccatctggac gacctcgaga cctgggcgcg caagctcacc
     2341 aaggtacgct tgagccgcct cttcgatttc gacttcggcc agggcggcat catcgccggc
     2401 cgccgcgtgc tgcagatcct ccatccctcg ctgctcgaga cgacgatcga gcgcttgagg
     2461 agccccttcg tctgcgtcgc caccgatctc aacaccgggc atgaggtatg gctgcgcgaa
     2521 ggcagcctca tcgaggcgct ccgcgcctcc tatgccatcc ccggcctctt cccgccggtg
     2581 gccctgggcg gccgttggct ggtcgacggg gccatggtga acccggtgcc ggtctcggtg
     2641 tgtcgcgcgc tgggcgcgcg gctgaccatc gccatcaatc tcaacgccga cgtcttcggg
     2701 ccggcgccca ccggcgagga tgcactcgag ctcgagcagc gtgccgtgcg cacggaggag
     2761 aaagacggcg gcggcttcat gcgcgcctat tttcgccgcc gcaacggctc gcccagcacg
     2821 ttcagcgtgc tcgcgcgctc gctgcacatc ctgcaggacc ggatcagccg gtcgcgtctg
     2881 gccggcgatc ccccggatgt catgatcgcc cccaggctcg gccatatcgg ctatctcgaa
     2941 ttctatcgcg ccgaggaatg catcgccgcc ggcgaagagg ccgcgcgccg cgccgtgccc
     3001 gagattcgcg aggcgattcg tcagctcggg acgagcagcg cctggaaccg caacgcgcga
     3061 ctccgtgccg gcgaaagcgg cgacgcggac ggctcggaat agaagtttgt ggttcccgat
     3121 gccggccgag tattgtcgcg cgcagatgac accgcctcgc cccacccgcg tcgccgaggc
     3181 cgctcgatga gcttcatcgg gcgctggctc ggcaattcga tcaatatcgc gctggcgctg
     3241 gtctttgcca tcgtcgcgat gcaggccccg gccttcacgc gcgactacgc cgaggcgctg
     3301 ctgcaggtgg cgcaggagtc gcgacgcgac atcgagcagc gcaaggactc ggcgcggcga
     3361 ttctatgcca tcccggccga gggggacgag caggtcatcg ccgccctcaa gcccttcgag
     3421 ccctccaatg ccgagaccct ggccctctcg gtcgatcgcg cgcggcgcct gcaggcggcc
     3481 tatgacaagg tcaccggaac caacccgctg ctgcagccga tcgcggcgtt gagcgatgcc
     3541 gtcgaggatg agcatggcta taagaccgcc gtcttgagga cgctgttcag cacctacgtc
     3601 ccgcatctgg atttcggcgt ggccgccgcg gcctacggac tggccggcct ggtgacgggc
     3661 tctttcgtgg cgcagcttct tgcggcgttg ggccggagga tcggcggccg gcgcctggcg
     3721 cgcgagggct gacgtcgcgg ccaataaggc gcgggcacaa gaaaagagcc ccgccctgag
     3781 ggcggggccc cgctcaacta cccggctcgc tctctagaga aggcatgact cttccgcacc
     3841 gggcgatttc gatcctcggg ccgaaacggt taatttttgg ttacgccgcg agccctctgc
     3901 ggaaccggta cgagcgtgtg ttgtgcttgc aacgacgaga gccgacgagc ggctaagatg
     3961 gagccgcgcc gtgtttcgca cgggtttcac cacggaggca cggagaagag gggttcggcg
     4021 cgcgaagcgc gccactcttc gcttctccgt gcctccgtgg tcgatctttc ctatcggcgt
     4081 ctcggcagga tatgcgattg ccctgggatc agtcggcgcc ggctggggcc tgggcccgag
     4141 aggcccggcg ctgcgccact tgcatcacgg cgggcacgcc ggcggcgaaa ctgcaggcaa
     4201 tggcgaggaa cacggcgtgc ggcgcgccgc gatcgatgag ggcgccgaac agcaacgggg
     4261 cgatcgacga gccgagatcg aagcccgaat agacgaagcc gaagacgctg cccatgcccg
     4321 agccggccgc cgcctggcgg atcagtacgt cgcgcgacgg cgcggtgatg ccgctcgcca
     4381 tgccggccag catcatgagc ggcacggcgg ctccccccgg caggcccgcg atcagcagca
     4441 tcagcgcggc ggcgagcgcg atgccgctca tcgccacgcg atgatgctgc cgcgtgcgct
     4501 cggcgaggaa gcccccggcc atcatgccga tggcgctgcc gatcaggtaa agggtcaagc
     4561 ccagcgtcgc gatgggcagt tcgaggccat agccggagac gagcgctgtg atcgagaagg
     4621 tctggatgcc cgtgccggca aaagcggtga gggcaaaata ggtgaagccc atcagcacca
     4681 cgggcgagcc gacgacgcgc caataggcga gatggggccg cgccgcgccg ggggcgggcg
     4741 ccggcacggc gcgccgataa tcgagaaggg cccggttggc gtagaggaga gccgccaccg
     4801 cgagaccgcc ggcgccgatt gccgtcagcg cgacgcgcca acccgcaaag gccgcgatca
     4861 aagccaccag gaccggcgcg gtcgcgaagc ccaggctgcc gcagatgccg tggaccgcat
     4921 aggcgcggcc gagccggctg acgccgacgc gatggctcag gatcgacaga tcggcgggat
     4981 gaaagacgct gttgccgagg ccgccgagca gcgcgagcgg cagcagcatc cgatagctgg
     5041 tcgcgaaccc catcaggccg atggcgaccg agagcagcac gaggccgccg aggaggagcc
     5101 ggcgcgcccc gtagcggtcg acgagaacgc cggcagcggc ctgacccagg cccgaactcg
     5161 catagaagat acccatgacg aggccgagat cggcaaagct caccgcgaaa tggtcatgga
     5221 gcagcgggaa cagcggcgcc agcgccagct gcatgacatg gctcgaataa tgcgccatgc
     5281 cggccaaggc gatgacggcc gcgtcgcggg gtcgctcttc gatggatcgg gctggggcgg
     5341 acatggccgg agacattacg ccggagacat tacctcaaca ccaacgctga gcagccatcc
     5401 ggcaccggaa tggctgccat gcaacacggc aggtggccgg ttcatgccgt gccagtcctg
     5461 ctcggtgcgg ctctcgcgtc cccgctttaa gcgggcacgc tgacgccgcc gcggacgagc
     5521 gcgcaggcgc gcgctcgccg gtgctatccc gcatggatgt cggaactgag cctgcccgat
     5581 gcgcgccgca ggccgatcct gccatcgcgg ctatgcagcc gaaagcccag cttggcgcag
     5641 aggacgagca cggcgtgagc gagctggacc cggcgcaggg gcgacaggaa ccagcgcggc
     5701 gcatagaaat cgccggccag gatcgcgatc aacccgttgc gcgtatcgaa caggtcgaat
     5761 tcctccatca acatgtcgcg caagatgggc gtattgatcc ggtagatcag ccaggcgaag
     5821 gcatcgagcc cgcgccgcac gcggcgctca tattcgcgca tcagcgtcgc cgcttccggc
     5881 ttgccatcga gctgcgccgc gaccgcctcg accgcgaagg cgccgctgct catcgccagc
     5941 atgacgccgc tggagaaaag cgggtcgatg aaggcagcgg catcgccgat gaggatgtag
     6001 ccgtcgccgg tgacacgggt tgaacgatac gaaaaattcg ccgctgagac gagcggcccg
     6061 accggctgcg ccgttgccat ccgctcggcc aggctcggcg tcgccgcaac ggcccgcgtg
     6121 aagaatgcgt cgagatcgcc ggtccgggat ttgaagaagg cctgggtccc gaccatcccg
     6181 acgctggtga tgccgtccgg cagcgggatc acccagaacc aaccctgcgg gaagagatgc
     6241 acggcgatca tgccttcggc attgccgtcg cgccgttgca catcgcggaa atggccgaac
     6301 agcgccgccg tattgttgcg cttatcggat tccttggtct cgaagcgggt ggccagcagc
     6361 gtgtctcggc cggaggcgtc gacaacgaaa cgcggccgcc actgtgcgac cgcgccgtcg
     6421 gccttgaccg ccgtcaccgt cgcgcggcgc ccggcgtcaa acgccatatc cgtgacgcgc
     6481 acgccttcct cgacggtcgc cccgcagcgg cggctgttgt cgagcaatag tttgtcgaaa
     6541 tcggcccgct tcacctgata ggcgtgcttg acggtcaagt gcggattggc gccgaaacgg
     6601 aagaccgtcg tcttgccgtg ctcgtcggaa aagacttgtg cgccgggctt gtacacgccg
     6661 agccgccgca cgtcgtcggc gacgccgagc cgatcgaaca cgggcaggtt cagcggcagc
     6721 agcgattcgc cgatatggaa gcgcgggaag aactcctttt ccagcagcac gacatgcttt
     6781 ccctgccgtg ccagcagcgc cgcggccgtc gacccggccg gcccgccgcc gacgacgagg
     6841 acatcgcaat cgacggcatt ttccatcgac agcctccgac gctgttccaa cgaatgaccc
     6901 gacgcgaacc gacgttattc ctaagattct gctcgtcctc agtgcggctc caagccgtgc
     6961 tagttctgct cgatgcggct ctcgcagttt gctgcgcaaa ctgctgccgc cgccgcggac
     7021 gagcgcgctg gggcgcgctc gccgagcgcc tcgccgaggg cgaagtggca ggcgacgaag
     7081 tggccgggtc caatcgcccg catcgccggt tcttcggcgg cacagcgcgg cgcggccatg
     7141 gggcagcggg tgcgaaaacg gcagccggat ggcggggcca tcggcgaggg cggctccccc
     7201 gtcaccacgc gctccggcag cggcgcctcg gggtcgggga cgggaatggc cgcccgcagt
     7261 gccgccgtat aggggtgacc cggcctgcgg aagacctcgt cggccggtcc gatctcgcag
     7321 agcttgccga gatacatgac ggcgacgcga tcgctgatcg ctttcacgac ggcgaggtca
     7381 tgggcgatga acagcagggt caggccgtag cgctgcttca tctcctcgag caagttcagg
     7441 atctgcgcgc ggatcgagac gtcgagcgag gagaccggct cgtcgcagat gatgagatcg
     7501 ggctcgagca ccagcgcccg cgcgatcgag atgcgctgac attgtccgcc cgagaactcg
     7561 tgcgggcgcc gccgcatcac gaggtcggca tcgagcccga cggcggcgag cgtcgcgcgg
     7621 acgcggcgct cgcgcgtcgc cgcatctttg acgccggcga tgatgagcgg ctcggcgacg
     7681 atgtcgccga cgcgccggcg cgggttgagc gaggcgatcg ggtcctggaa gatgagctgc
     7741 aggcggcggc gcaggcgccg caacctttcg ccgcgctcgc gggtgaggtc gatgccgtcg
     7801 aacagcacgc gcccggcatt cggccgcacg agctgcagca cggcgcggcc cagggtcgac
     7861 ttgccgcagc cggactcgcc gacaagcgcc agcgtctcgc cgcgcgcgat atcgaagctc
     7921 acatccgaga cggcctggac gcggtaattt ccggtgcggt acgcgaccgt cagattctcg
     7981 acgcagagca gtggggctcg cccggccgcc gcgatggcga cgggtgccgc ctcgctcata
     8041 gcgcgctgcg ccggggatcg atcgaggcgc gcaaggtatc gccgatcaga ttgatcgaga
     8101 tcacggtgag gaacatggcg gcggccggaa tgaacgccac atgcggcgcc acggcgaggc
     8161 tgtcacgccc ttcggcgatc atgctgcccc agctcggcgc cggcggcggc acgcccaggc
     8221 cgagatagct cagaatgccc tcggcgatga tcgtcaccgc ggccagcagc aggaaaaaag
     8281 cgccgagcgg cacgaggaca ttgggcagga tctcgcgccg caggatgcgc gcgtggctgg
     8341 cgcccaacgc gcgcgcggcg atgacgaatt cgcgctcgcg cagcgacagc gtcgttgccc
     8401 gtgccacgcg tgtcacggcc gggatcgtca ggatgccgag cacgatgatc aggttcacga
     8461 ccgacgcgcc gagataggcg atgatggcga gcgcgagcac cagcggcggg aaggcgagca
     8521 gcacgtcggt cgcggcgatg gcgagggtct cgaaccggcc gcggaaatag cccgcgagaa
     8581 cgccgagcac cgttccgacg acggtgccca ggagcggcgc cgtgaagccc acggccaagg
     8641 agatgcgggc gccatggatg aggcgggaga ggacgtcgcg gcccagcccg tccgtgccga
     8701 agaggtgccg agcgctcggg ctggcgcggc gtgccatcaa ggtctgcttg aaggggctgg
     8761 cgagcggcaa gaggtctgcg gccaatgcca gcaacaggac gatcgcgatc caggcgacgg
     8821 ccagccagaa cagggcaccg aggccggggc gccgccgttc cgccgcggcg acaggcactt
     8881 cgcggtcgag ggcggcgtca agcgtggcca tggcgaatcc gcggatcgag cgcggcatag
     8941 agcaagtcga ccgcgaagtt gacgacgacg aagccaatgg cgacgagcac gacgccgccc
     9001 tggaggatga tgaagtcgcg caggaagatc gcctcgacca gcaggcgccc gacgccgggc
     9061 aacgcgaaga tcgtctcgac cagcagcgcg ccgccgatga gccgtccgat attgaggccg
     9121 acgatcgtga cgagcgtcag cgacgagggc ttcagcgcat gcacgagaag gatgcgccgc
     9181 tggctcagcc ccttcgcctt ggccatggcg atgtagttct cctgcagcgt cgagatcatg
     9241 tcgctgcgca ggatgcgcat gatcgccggc cattcgagga gcgccaaggt caggccgggc
     9301 agcgccatcg cgcgcagatt gccgaggata tcctccgaca gcggcacgta gcccgtcgcc
     9361 ggcagccagt tgagctgtac cgcaaagaga aagatcagca ggatggccag caggaagggc
     9421 ggcaccgaga gcgtgccgaa agcggcagca gacgcgacgc gatcgagctc cgagccggct
     9481 cgcaccgcgc agaggatcgc cagcggcacg ccgatcagga gaccgcccag ctcggcgatc
     9541 accatgagct cgagcgagac ggggagccgg cccgcgatcg catgcagcac ggtctcgccg
     9601 gtccgctgcg aggtgccgaa atcgccggag agcgcaccgc cgagccaatg gagatattgc
     9661 tgccagagcg gcaggtcgag gccgagcttc ttgtgcagcg ccgccactgc ctcgggcgtt
     9721 gcctggtcgc caagcatggt gaccgcgagg tcgcccggca gcagcgaggc gatgaagaag
     9781 gtgaggacgg tcacggccag gacgaccgga atcagataga gcaaccggcg gccgacgaag
     9841 ccgagcatcg cgggccggcc tctatttctt gtcccaccat gcggtggtca agtcgatgac
     9901 accgctgcgc tgctgcggaa tcccttcgag atgcgccttg gcgatggcga aggaatggat
     9961 ctcgagcgcc cagaaccacg gcacgtcctt gttcaggatc cgggcgatct cgcaataatc
    10021 gtcgcttctc ttctccaggt cacccgaact acgggcgtcc tcgagcagcc gatccacgtc
    10081 gggattcgaa tagccggcga gattgaccgg gctcccggtg tggaaattcg cgtacatctg
    10141 cggatcgggg tcggcggaat cgatgatgcc ccagggagcg acctggaagt tgtggctgaa
    10201 gaccctggcc ccgagcgtgc tctggtcgat ctggtcgatg ctcaccttgg cgccgaccgc
    10261 gctccagaac tcctggaaga tctggccata ggtgcggccg cgcggcgtcg ccgtcacgat
    10321 caacttgaaa tcgacgggct tgccatagtc cgcgagcagc gcctttgcct tggccacatc
    10381 gtaaggcagg agcccggtgt ccttgcactg cacgaacgag cccggaccat aaggatcctt
    10441 ggcggccttc gacacgccgc tcgccacggc gtcggcaaag gccggcatgt ccatcgccat
    10501 gcgcatcgcc tggcgcacgc gcacatcgtc gaagggcggc tgcctcatgt tgaagacata
    10561 gaccaccgcg cccgagcccg cgtattcgcg cacggcaaag tttgcgttct tgcgcgcgtc
    10621 ggcgatgttg tcggcattgt cgtcccagat cacgtcgctc tcgccggatt gcaggctggc
    10681 gtagcgtgcc tgggcatcgg tcagcgggcg caccgacact tcgtcgagat agacgtgatc
    10741 cttgtcccag tagtcgggat tgcgctcgag cacgatctgg tcgcccgatt gccaggactt
    10801 gagccggaac gcgccagtgc cgacgggatg gcggttatag ccctccttca tctcttcgat
    10861 ggccttgggc gagtggatca cggtggtgac ggtcttcggc gcaatcaagg cggcccagtt
    10921 gggctgcggc gcgttcaggt gatagacgac cgtcagatcg tccgcggctt cgatggtgtt
    10981 gatcgcggtg atgtaggcag cgcagcggca gtgattgtcg gggttgcgca tgcgttgaaa
    11041 gttgaaggcg accgcctggg cattgaaggg cgagccgtcc tggaact
//
LOCUS       DASYNS010000327         4700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4291581, whole genome shotgun sequence.
ACCESSION   DASYNS010000327 DASYNS010000000
VERSION     DASYNS010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4291581"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VGP50_10095"
     CDS             complement(<1..478)
                     /locus_tag="VGP50_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-4 component E"
                     /protein_id="HEV8027773.1"
                     /translation="MPTSPNISYDTAHVLDSAVLVLSFALLYQRRLFAVLNIYAYQAL
                     FLAVAAAWQAYAQQAPHLFITAAIALVFKAAVIPVALHRIVLRLDIHGGIETALGVGA
                     TLVVGVALVALSVLLVLPVTTTSGAVTRESLAIALSVVLLGLLMMITRRNAVTQVVG"
     gene            complement(488..1435)
                     /locus_tag="VGP50_10100"
     CDS             complement(488..1435)
                     /locus_tag="VGP50_10100"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEV8027774.1"
                     /translation="MTAVLLELLQLTLLVALAPLLTGIIRKVKARLNARIGPALLQPY
                     RDLWRLLRKEVVLAPNASWLFRTSPYLIFTAIWIAAAIVPTFASGLLLSRTADLIALV
                     ALLGTARFALALAAMDVGTSFGGIGASREMMIASLAEPAMLMVTFTVALVVRSTSLPH
                     IAEFLLTHDVGIRVSLLLGLLSLVMVAIAENARIPVDNPATHLELTMVHEAMVLEYSG
                     RHLALIEAASMLKLLLYLSLIASVFFPWGMAIGPLGTAGLAVGLAAYIAKLITGSILL
                     AVFETSIAKMRVFRVAEFLGGALLLGLLAAIFLYVSQQV"
     gene            complement(1432..3426)
                     /gene="hyfB"
                     /locus_tag="VGP50_10105"
     CDS             complement(1432..3426)
                     /gene="hyfB"
                     /locus_tag="VGP50_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEV8027775.1"
                     /translation="MLSLVAIVLATYGAAALLGMTGRRALVAVYGLSALAAAATAVAA
                     ARFLASGASPEAAVLPLGLPWIGTHLRIDALSGFFLLVIGVAGTLISIFALEYGRHDT
                     EPQRVLPFYPAFLAGMTLVLVADDAFTFLLSWEFMSLASWLLVLANHRETGTSSAALL
                     YLVMASFGVGALLLAFGLLAGGTGDYAFEALRGQSLAPWESGLLFALALVGAGSKAGV
                     VPLHVWLPPAHAAAPSHVSALMSGVMTKVALYGLVRLLFDLAGPPPWWWGVTVLLLGG
                     ITALMGVLYAVMEHDLKRLLAYSSVENIGIIVIGLGLALAFRANRLDDLAVLALAAAL
                     FHIFNHAMFKSLLFLGAGAVLVATEDRNMEHLGGLIQRMPATAFVFLIGAVAISGLPP
                     FNGFVSEWLAFQAILNGSLLVQWLLKFAVPVVGAMLALATALAAVTFVKAFGIVFLSR
                     PRRPAAANAHEVGLLMLVPMAVLAAICTALGAVPQVLLLVLPPVARSLWPDSPPLMLS
                     NWLWLAPLGPDHSSYSGLVILVAVGLLAVLLVFVIHRFASNRVRRSAPWDCGFPDPRP
                     ETQYTSSSFAQPIRRIFGSAAFAAREHIDMPLPGDTRPARFEVSLRDPVWDNLYAPVT
                     FAIAWLADRLNVLQFQTIRRYLSLMFAALVLLLLVVAVSQ"
     gene            complement(3612..3980)
                     /locus_tag="VGP50_10110"
     CDS             complement(3612..3980)
                     /locus_tag="VGP50_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV8027776.1"
                     /translation="MKRTTSRTQDIDRHVGARIRERRIMLGLTQQQLADLIGVTYQQA
                     HKYERGINRVSAGRLFEIARVLSVPVSYFFEGLDNQSGRPVSPRERMCLELARNFAQI
                     PNERHQEALSQLARALATQH"
BASE COUNT          949 a         1449 c         1647 g          655 t
ORIGIN      
        1 atcccaccac ctgggtcacc gcgttccgcc gcgtgatcat catgaggaga ccgagaagca
       61 cgaccgacaa ggcgatcgcc agactctcgc gggtgacggc gcccgaagtg gtcgtcaccg
      121 gcaacaccag caagacggag agcgcgacga gcgcgacgcc cacgacgagc gtcgcgccga
      181 cgccgagcgc ggtctcgatg ccgccgtgga tgtcgaggcg tagaacgatg cgatggagcg
      241 ccaccggaat tacggccgcc ttgaacacga gggcgatcgc ggccgtgatg aagaggtgcg
      301 gcgcttgctg cgcataggcc tgccaggcgg cggcgacggc gaggaacagc gcctgatagg
      361 cgtagatgtt cagaacggcg aaaaggcggc gctggtagag aagcgcgaag ctgaggacga
      421 ggacggcgga atcgagcaca tgtgccgtgt cataggagat gttcggggac gtcggcatcg
      481 ttgtgcccta cacctgctgc gacacgtaga ggaagattgc agcgaggagg ccgaggagca
      541 gcgcgccgcc caggaattcg gcgacgcgga agacgcgcat cttggcgatg ctcgtttcga
      601 agacggcgag gagaatgctg ccggtgatca gcttcgcgat ataggcggca aggccgacgg
      661 cgagcccggc ggtgcccagc gggccgatcg ccatgcccca ggggaagaag acgctggcga
      721 tgagcgagag gtagagcagc agcttcagca tggaagccgc ttcgatgagc gccagatggc
      781 ggcccgaata ttcgaggacc atcgcctcat gcaccatcgt cagctcgaga tgcgtcgccg
      841 gattgtcgac ggggatgcgc gcattctcgg cgatcgccac catcaccagc gaaagcaggc
      901 ccaggagcag cgagacgcgg atgccgacat catgcgtcag caggaattcc gcgatatgcg
      961 gcagcgaggt cgagcgcacg accagggcca ctgtaaaggt caccatgagc atcgccggct
     1021 cggcgaggga ggcgatcatc atctcgcggc tcgcgccgat gccgccgaag ctggtgccga
     1081 catccatggc ggcgagcgcc aaggcgaagc gcgcggtccc gagcaacgcg acaagagcga
     1141 tgaggtcggc cgtgcggctc agcagcagtc cgctcgcgaa agtcgggacg atcgccgcgg
     1201 cgatccagat cgcggtgaag atcagataag ggctggtgcg gaagagccag gacgcgttgg
     1261 gcgcgagcac cacctccttg cggaggagcc gccagagatc gcgatacggc tgcaacaggg
     1321 ccggcccgat ccgagcattg agccgcgcct tgaccttgcg aatgatgccg gtgagcagcg
     1381 gcgccagcgc caccagcagc gtcaactgca ggagctccag cagcacggcg gtcattggct
     1441 caccgccacc accaggagca gcaacaccag tgctgcgaac atcaggctca gataacggcg
     1501 gatggtctgg aactgcagga cgttcagccg gtcggcgagc caggcgatcg cgaatgtcac
     1561 cggcgcataa aggttgtccc atacgggatc gcgaaggctc acctcgaacc gggccgggcg
     1621 cgtatcgccg ggaagtggca tgtcgatgtg ttcgcgcgcg gcgaaggcgg ccgaaccgaa
     1681 gatgcggcgg atcggttggg cgaaactgct cgacgtgtat tgcgtctcgg gccgcggatc
     1741 ggggaaaccg caatcccacg gcgccgagcg gcgcacgcgg ttcgacgcga agcgatgaat
     1801 gacgaagacg aggagcaccg cgagcagccc gaccgcgacc agaatgacga gcccgctata
     1861 ggagctgtgg tcgggaccga gcggcgcgag ccagagccag ttcgagagca tgagcggcgg
     1921 gctgtcgggc cataggctcc tcgccaccgg aggcagcacc aggagcagca cctgcggcac
     1981 cgcgccgagg gcggtgcaaa tcgccgccag cacggccatc ggcaccagca tcaggagacc
     2041 gacttcgtgg gcattggccg ccgccggtcg gcgcggtcgg ctgagaaaaa cgataccgaa
     2101 cgccttgacg aaggtcacgg ccgcgagggc cgtcgccagc gccagcatcg cacccaccac
     2161 cggcaccgcg aatttgagca gccattgcac caggagcgag ccgttgagga tggcttggaa
     2221 agcgagccat tccgagacga agccgttgaa gggtggaagc cccgagatgg ccacggcgcc
     2281 gatcaggaag acgaaggccg tcgcgggcat gcgttggatg agcccgccca gatgctccat
     2341 gttgcgatcc tctgtcgcca ccaggacggc gccggcgccg aggaagagca gactcttgaa
     2401 catggcgtgg ttgaagatgt ggaagagggc ggcggcgagc gccagcacgg cgaggtcgtc
     2461 gaggcgattg gcgcggaagg cgagagcgag gcccaagccg atgacaatga tgccgatgtt
     2521 ctcgacgctc gaataggcga gcagccgctt caggtcatgc tccatgaccg cgtagaggac
     2581 gcccatcaac gcggtgattc cgccgagcag gagcaccgtg acgccccacc accagggcgg
     2641 cgggccggcg agatcgaaaa ggaggcggac gagcccatag agcgccacct tggtcatgac
     2701 gccgctcatc agtgccgaga catgactggg tgcggcggca tgcgccggcg gcagccagac
     2761 atgcaacggg acgacaccgg ccttggagcc ggcgccgacc agcgccagcg caaacaggag
     2821 gccgctttcc cacggcgcga gcgactggcc gcgcagggct tcgaaggcat agtcgcccgt
     2881 cccgcccgcc agcagtccga aagccaggag cagcgcgccc acgccgaaac tcgccatgac
     2941 caaataaagg agagcggcgc tcgatgtccc ggtctcgcgg tgattggcga gcaccagaag
     3001 ccatgatgcc agcgacatga actcccagct cagcaggaaa gtaaaggcat cgtcggcgac
     3061 aagcaccagg gtcatgccgg cgagaaaggc cggatagaag ggcagcacgc gctggggctc
     3121 ggtgtcgtgg cgtccatatt cgagggcgaa gatgctgatg agcgtgccgg cgacgccgat
     3181 caccagcagg aagaagcccg agagcgcgtc gatgcggaga tgcgtgccga tccagggaag
     3241 tcccaagggc agcacggcgg cttcggggct tgcgccgctg gcgaggaacc gcgcagcggc
     3301 caccgccgtc gcggcggcgg caagcgcgct caagccatag acggcgacga gcgcgcgccg
     3361 tccggtcatg cccaggagag cggctgcgcc ataagtcgcg aggacgatcg cgacgaggct
     3421 cagcatatga gaaaagaagg cccggacgtc gggcttggag cggcggggct agcgggcgct
     3481 gccttgaacc ggcagcccgc ggcggacgga gccatcaggg gtgagcgcgc gacctgaaaa
     3541 aggcattcta tcagccgccg ctcacggggc gcaaaccgct ccgccgccca ggggtgcggc
     3601 ccctcgccgc gtcagtgctg cgtcgccagg gcgcgcgcga gctggctcag cgcttcctga
     3661 tgccgctcgt tgggaatttg cgcgaagttc cgcgccagct ccaagcacat gcgttcgcgt
     3721 gggctgaccg gacggccgct ctggttgtcg aggccctcga agaaatagct gacgggcacg
     3781 ctcagcaccc gcgcgatctc aaagagtcgt ccggccgaca cgcgattgat gccgcgctcg
     3841 tatttgtggg cttgctggta ggtaacgccg atcaggtcgg cgagctgctg ttgcgtcaag
     3901 cccagcatga ttcggcgttc gcggatccgg gcgccgacat ggcgatcgat gtcctgcgtc
     3961 cggctcgtcg ttctcttcat ttcggtcgca ctcatggctt ttgacacgcc gtcaaaggga
     4021 ggggtcggtg tgccggacgg cgcccgacaa gcccatcagg gaacgtgcga tcgtgtgaag
     4081 gtgcgcgccg tcggacaaag ggaaaccaaa ggtttccctg ggtcgtctcg gcataaaaca
     4141 aaactatgtg ctgatcgtcc ggatttcaac cagtttttcg ccgaaaatgc gaaagacgag
     4201 cgggattgga aatttcgagt acatcgggaa ctccacagca gggccggaga aatattggcg
     4261 gggtcgacct ggcttgtctt gcggaacgcg ctcgaatact ggcaccgccc gatggggacc
     4321 ggacagcgac cgggatcggg gagaagcacc gcgccggcta ggctttccac ggccattccg
     4381 catccttcgc taagctcggc cgcagccgcc tgcccactgt cgacataggc gccgccaaca
     4441 gaattttttg gttcggccgc tcgatcgcta acactcgttg cgtgatgagg ttccgctact
     4501 acggcgcgac ctccgattgt cgcagcgcgc cgctcgctgt tccgaaggcg tcgcgatcgt
     4561 cgtgccgagc ccttgcatcg tcccccggca ggctcgtttc gacgctgccg cgcgtcggcg
     4621 ggattgtcgc gtgtcaaggg gccaggcccg catccctgcc gctcgcgcat gactgcatat
     4681 cctgcgcgat gtttgacagc
//
LOCUS       DASYNS010000328         7864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3044481, whole genome shotgun sequence.
ACCESSION   DASYNS010000328 DASYNS010000000
VERSION     DASYNS010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3044481"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VGP50_10115"
     CDS             <1..700
                     /locus_tag="VGP50_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027777.1"
                     /translation="SGQGGFDGGINNWVPKAGGILGQTNTTEVQGDVVTDWDKFSGPN
                     QGCPVPMLPLQDATTTAGQTAVQNTINAMWPRDAGGTQVPIGMIWGWRMLSANGPFPQ
                     NNGHPLTYANQATLAWKKAVILMTDGTEEWPSTQQDTGLSYISDGKIGTTNNTNTAQN
                     NINTRLTTLCNNMKATGNFVIYTIGLGSDGASNTQLQQCATSTGGFYVATPSNLNTVF
                     QSIANSLLTLRLSQ"
     gene            complement(793..2703)
                     /gene="nhaA"
                     /locus_tag="VGP50_10120"
     CDS             complement(793..2703)
                     /gene="nhaA"
                     /locus_tag="VGP50_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEV8027778.1"
                     /translation="MADESGAAPNDAVRLKRAIDPVRDHMRGAQVADGVASVVFYGDY
                     LCPYCRGLGPVLARLRQVFGNRLAYVFRHFPNERAHPGATFIHRAAEAAGRQGRFWEM
                     HDRLYDEEPQLDESRVLAITGELGLDMECFRRDLASDDTARRVDDDRAEGRRNGVTGT
                     PTIFVDGLRYDGAWDFYSMLEALERPVAARLQRSARVFASLPASGGLVLILAAAAALF
                     CANTPLAPFYRLFVDAPFSIGPPGGALTLTVGDWFSQGLLAFFFLLVGLEIRREMTAG
                     ALATPRAALLPVIAALGGMLAPAAIYLALNRGPTAPGWSVPTATDIAFALGILALLGD
                     RVPVGLRVFVAALAVVDDLLSMLTLAVFYPHVFDATWLVAAALAAALLFALNRARVYA
                     AWPYVATTLTLWFCFHAAGVHAALAGVALAAFPPTRPAPAAGPLLAQAATALAALEDV
                     ENEAKQRGGEARRIEQEPIWDWASRNLSAASQRFLSPADRAERAVAPWSAHLVLPLFA
                     FSATGIAFDVDLSSPGARAIFSGVVLGLLVGKPLGVALASLLAVKARLALTPEGVTLR
                     QFIGGACLCGIGDTVALLMADQAFPQGLDAAVAKIGVLAGSVLAAALGAAILAGGAKA
                     RDATPASAASSG"
     gene            complement(2840..3643)
                     /locus_tag="VGP50_10125"
     CDS             complement(2840..3643)
                     /locus_tag="VGP50_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027779.1"
                     /translation="MTVSALGPYHVTALNTAKHSENLIHDDAVARRFGFAGGLVPGVE
                     VYAYMTHLPVARWGRAWLERGTAECRLLKPVYDGDALTLTASATADGLELHLAKGGEL
                     CASGRAALPTAASPVPDAFAAAPLPPVPPANRPPADEATLAPGLWLAIHPFRVGADAA
                     RQYLKDVGETLPVYESAALVHPGTILHIGNWALRHNVVLGPWMHVGSRIEHVRAARIG
                     EELSARALVTANYERKGHRFVELDVLVYASGSTPLARIAHTAIYRPRGA"
     gene            3757..5118
                     /locus_tag="VGP50_10130"
     CDS             3757..5118
                     /locus_tag="VGP50_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEV8027780.1"
                     /translation="MQLRDFPGTLALGEGSATEMMQGQLRIWLERIGLFGPGVVLTLA
                     AFAVAYYFVQPAPPRHIVMATGDADGVYYYYGQIYRDLMAREGVEVTLAETAGSIDNI
                     ALLTSGKADVGFVQGGTRNDSGHTELRSLASLYFEPIWVFVRRDLKALRLADLRGHRV
                     AVDREGSGTRVMALQLLADTGLDGTNVEISAQGHSDAAKAMHEGALDAAFFIAPANAP
                     FLRDLFVDRRLRLLHIDEAAAYRLQHRFLSVLTLPQGAIDLAARVPDADTVLLAPAAN
                     LVVREDFHPALAELMLIEARRIHRDPGVFEDAGQFPSREHLEYPIADAAQRFFDSGPS
                     LLRRYLPFWAANLADRLKIMLVPLVALAYPLFKIIPPTYDWRMRSRIDRWYKELQVIE
                     RKIEATTTKDELSRRLSELTALEHNVRRLTMPVAYGNPLYSLRQHIVMLRAELESAVE
                     RGT"
     gene            5131..6237
                     /locus_tag="VGP50_10135"
     CDS             5131..6237
                     /locus_tag="VGP50_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027781.1"
                     /translation="MFACSSLALPPNAAIQRDIARAAEFRPADFMDKFDAHTLFYDFI
                     ATGKEREHSWLLIAPPLLNLLPLLAQASINGQRIGALVTQYYARYLCCDVWMINVSSE
                     SVLWKFAFGACPIEAQPSQNHLYEGKRVLYTLSKDNDLRWIADWVRFHVRNHGADAVL
                     IYDNASTRYSGEDLEQALRSENPSLEVNVVNWPFKYGPQGIDDRWWDSNFCQSGALHD
                     ARFRFLERARSVLNCDIDELVVSETGGSVFAATEKADGGYLLLGGRWMASTAETPAGA
                     LPRHADFRYLDAAAAACPTKWCVVPAKCALERQWHTHGVGAKPVELNRTFSYRHFRSI
                     STDWKYPRTPLAAYDSRIHERDGPLSEAMRRAGL"
     gene            6377..7573
                     /locus_tag="VGP50_10140"
     CDS             6377..7573
                     /locus_tag="VGP50_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV8027782.1"
                     /translation="MRIHLMAMTALLAGAASAAVAEEKPIKIALIADKTGPLEAYAKQ
                     TSAGLAMGLQYATGGTMTVAGHKIEVIEKDSQTKPDVGRTLLGEAYNDDGADIAIGGT
                     SSAVALAMLPVAEDNKKIFIVEPAVADSITGDKWNRYIFRTGRNSTQDAVSNALAVGK
                     PGTVVATLAPDYAFGHDFVTAFGTALAPTGAKLVYEEYAPPGTTDFTAPYQRILDALA
                     KEQGRKIIFINWAGGGNPMSALRALDPERLGIALATGSNILPALVAYKDFPGMEGAGY
                     YYYENPKNAVNDWLIAENRKRFDGAPPDFFTCGGFIAGEAVVEALKRTQGSTDTEKLI
                     TAMEGMAFDSPKGRVMFRKEDHQLLQPMYAFKIKVDPKVPWAIPELVREIPISEMKIP
                     IMNHRP"
     gene            7599..>7864
                     /locus_tag="VGP50_10145"
     CDS             7599..>7864
                     /locus_tag="VGP50_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8027783.1"
                     /translation="MTGERAPVLATSDLTIRFGGQIAVNGVSCAFAEGSLTAIVGPNG
                     AGKTTYFNLISGQLRPSAGSVRFLGRDVTPLGAPERTRLGLGRS"
BASE COUNT         1397 a         2624 c         2672 g         1171 t
ORIGIN      
        1 gtcgggccaa ggcggcttcg acggcggcat caacaactgg gtccccaagg ccggcggcat
       61 ccttggccag acgaacacga ccgaggtgca aggcgacgtg gtcacggact gggacaagtt
      121 cagcggcccg aaccagggct gcccggtgcc gatgctgccc ttgcaggacg cgacgacgac
      181 cgccggccag acggcggtgc agaacaccat caacgcgatg tggccgcgcg acgccggcgg
      241 cacccaggtg ccgatcggca tgatctgggg ctggcgcatg ctgtcggcca acgggccctt
      301 cccgcagaac aacggccacc cgctgaccta cgccaaccaa gcgacgctgg cctggaagaa
      361 ggccgtcatc ctcatgaccg acggcaccga ggaatggccg agcacgcagc aggataccgg
      421 tctcagctat atcagcgacg gcaagatcgg cacgaccaac aacacgaaca cggcccaaaa
      481 caacatcaac acccggctca ccaccttgtg caacaacatg aaggccaccg gcaatttcgt
      541 gatctatacg atcggcctcg gcagcgacgg cgcgagcaac acgcagctgc aacaatgcgc
      601 gacatcgacc ggcggcttct acgtggcgac gccgagcaac ctcaacacgg tgttccagtc
      661 gatcgcgaac tcgctgctga cgttgcgcct gagccaatag cgcgacggct ttcaaaaacg
      721 tcgagctcga gcagggcgcc gcaaggcgcc ctgcttgcgt ttgggcgaga agatcgtccg
      781 cggcggcgag gatcaacccg atgaagcggc ggaggccggc gtcgcgtcgc gggctttggc
      841 gccgccggcg agaatggccg cacccagcgc ggccgcgagc acggagccgg cgagcacgcc
      901 gatcttggcc accgccgcgt cgagtccttg gggaaaggcc tggtcggcca tcagcagcgc
      961 gaccgtatcg ccgatgccgc agagacaggc gccgccgatg aattggcgga gcgtcacgcc
     1021 ttcgggcgtc agcgccagcc gcgccttgac ggccagcagc gacgcgagtg cgacgcccaa
     1081 gggcttgccg acgaggaggc cgaggacgac gcccgagaaa atcgcgcgcg ccccgggcga
     1141 cgacagatcg acatcgaaag cgatcccggt tgcggagaag gcgaagagcg gcaagaccag
     1201 atgcgcgctc caaggcgcca ccgcgcgctc ggcacggtcg gccggcgaca ggaagcgctg
     1261 gctcgcggcg gaaagatttc ggctcgccca atcccagatc ggctcctgct cgatgcggcg
     1321 cgcctcgccg ccgcgctgct tcgcctcgtt ctccacatcc tcgagagcgg cgagcgcggt
     1381 cgcggcctga gcgagcaagg ggcccgcggc cggcgcgggc cgcgtcggcg gaaaggcggc
     1441 aagcgccacg cccgccagcg cggcgtgaac accggcggcg tgaaagcaaa accacagggt
     1501 gagcgtggtc gcgacatacg gccaggcggc atagacgcgc gcgcgattga gggcaaagag
     1561 cagcgccgcg gcaagcgcgg cggcgacgag ccaggtcgcg tcgaagacat gcgggtagaa
     1621 gacggccagc gtcagcatgg acaagaggtc atcgaccacg gccagcgccg ccacgaagac
     1681 ccgcaagccc accggcacgc ggtcgccgag caaagcgaga atgccgagcg cgaaagcgat
     1741 gtccgtcgcc gtcggcaccg accagccggg cgcggtcggt ccccgattga gcgccagata
     1801 gatcgccgcc ggcgcgagca tgccgcccag cgcggcgatg acgggcaaca gcgccgcccg
     1861 cggcgtcgcc agcgctcccg cggtcatctc gcggcgaatc tcgaggccga caaggagaaa
     1921 gaagaaggcg agcaggccct gggagaacca gtcgcccacg gtcagcgtga gcgccccgcc
     1981 gggcgggccg atgctgaagg gggcatcgac gaagagccgg taaaagggcg cgagcggcgt
     2041 gttcgcgcag aaaagcgccg ccgccgcggc aaggatcagg acgaggccgc ccgacgccgg
     2101 caggctggcg aagacgcgcg ccgagcgctg cagccgcgcc gccaccggcc gctccaacgc
     2161 ctcgagcatc gaatagaaat cccaggcgcc gtcgtagcgc aacccgtcga cgaagatggt
     2221 cggcgtgccc gtgacgccgt tgcgccggcc ttcggcgcgg tcgtcatcga cgcgacgcgc
     2281 cgtgtcgtcg ctggccaggt cgcggcggaa gcactccata tcgaggccaa gctcgcccgt
     2341 gatggcgaga acccgcgact cgtcgagctg cggctcttcg tcgtagagcc ggtcatgcat
     2401 ctcccagaag cggccctgac gccccgcggc ctcggcggcg cgatggatga aggtcgcgcc
     2461 gggatgcgcg cgctcgttgg gaaagtgacg gaagacatag gcgaggcggt tgccgaagac
     2521 ctgccgcaaa cgcgcgagga ccggaccaag gccgcggcaa taggggcaga gataatcgcc
     2581 atagaagacc acgctggcga cgccgtcagc gacttgcgcg ccgcgcatat ggtcgcggac
     2641 gggatcaatg gcgcgcttga gccttaccgc gtcgttgggt gctgcaccgc tctcgtccgc
     2701 catcgtgtca gtgacccgga tatgctcgga ggtgacgcgg tctgtgtatc cgaagcaccg
     2761 cgccccgtcg agagtctcgc cccgaggttt gtgccgatgg cgcgcggcgg ctactgtccg
     2821 ctccgtcgag ccgccaggct catgcgccgc gcggccgata gatcgcggta tgggcgatgc
     2881 gggcgagggg ggtgctgccg ctcgcataga cgagcacatc gagctcgacg aagcgatggc
     2941 ccttgcgctc gtagttggcg gtgacgagcg cgcgcgccga aagctcctcg ccgatgcgcg
     3001 ccgcccgcac atgctcgatg cggctgccga catgcatcca tggcccgagc acgacattgt
     3061 ggcgcaaggc ccaattgccg atgtgcagga tcgtccccgg atggacgagc gctgcggatt
     3121 cgtagacggg cagggtctcg cccacgtcct tcaggtattg gcgggctgcg tcggcgccga
     3181 cgcgaaaggg atggatcgcg agccagaggc cgggcgcgag cgtcgcctcg tcggccggcg
     3241 gccggttcgc gggtggaacc ggcggcagcg gcgctgcggc gaaggcgtcg ggtacgggcg
     3301 atgcggcggt ggggagcgcg gcgcggccgc tggcgcagag ctcgccgcct ttcgcgaggt
     3361 gaagctcgag cccatccgct gtcgcgctcg ccgtgagcgt gagcgcgtcg ccgtcataga
     3421 cgggcttcag caaccgacac tccgccgtgc cgcgctcgag ccaggcgcgg ccccaacgcg
     3481 cgacgggcag atgcgtcatg taggcataga cctcgacgcc gggcacgagc ccgcccgcga
     3541 agccgaagcg gcgggcgacg gcatcgtcat ggatcaggtt ctcgctgtgc ttcgccgtat
     3601 tgagggcggt gacgtgatag gggccgagag ccgaaacggt catcactggg tgttccctgc
     3661 ggcggattgc gacggtcggc gctaggcttg caccacgccg ggtgcctgac aagccttgct
     3721 ttctgccgcg atgcggcagg ggggtcgttg cgccgcatgc aattgcgaga tttccccggt
     3781 acactcgcgc tcggcgaggg gagcgcgacg gagatgatgc aggggcagct ccgcatctgg
     3841 ctggagcgga tcggcctgtt cgggccgggc gtggtgttga cccttgccgc cttcgccgtc
     3901 gcgtattact tcgtccagcc ggcgccgccg cgccatatcg tgatggcgac gggcgacgcg
     3961 gacggcgttt attactatta cgggcagatc tatcgcgacc tcatggcgcg cgaaggggtc
     4021 gaggtcacgc tcgcggagac ggcgggctcg atcgacaata tcgcgctcct cacctccggc
     4081 aaggcggatg tcggcttcgt ccagggcggc acgcgcaacg atagcggcca caccgagctc
     4141 cgctcgctgg cgagccttta tttcgagccg atctgggtct tcgtccgccg cgatctgaag
     4201 gcgctacgtc tcgccgatct tcggggacat cgcgtcgcgg tcgaccgcga gggcagcggc
     4261 acgcgcgtca tggcgctgca attgctggcc gacaccgggc tcgacggcac gaatgtcgag
     4321 atttcggcgc agggccatag cgatgccgcc aaggcgatgc acgagggcgc gctcgacgcg
     4381 gcgttcttca tcgccccggc gaatgcgcct ttcctgcgcg atctcttcgt ggaccgccgc
     4441 cttcgcctgc tgcatatcga cgaggcggcg gcctatcgcc tgcagcaccg gttcctctcg
     4501 gtgctgacgc tgccgcaagg cgcgatcgat ctcgccgccc gcgtgccgga cgccgacacg
     4561 gtgctgctgg cgcccgcggc caatctcgtg gtgcgcgagg attttcatcc ggcgctggcc
     4621 gagctcatgc tgatcgaggc gcgccggatt caccgcgatc ccggcgtctt cgaggacgcg
     4681 ggccagttcc cctcgcgcga gcatctcgaa tatccgatcg ccgatgcggc gcagcgattc
     4741 ttcgactcgg ggccgtcgtt gctgcggcgc tatctgccgt tctgggcggc caatctcgcc
     4801 gaccgcctca agatcatgct cgtgccgctg gtggcgctcg cctatccgct cttcaagatc
     4861 attccgccga cctatgactg gcgcatgcgc tcgcgcatcg accgctggta caaggagctg
     4921 caggtgatcg agcgcaagat cgaggcgacg acgacgaagg acgagctgag ccggcggctc
     4981 tccgagctca cggcgctcga gcacaatgtt cggcgcctga ccatgccggt cgcctatggc
     5041 aatccgctct acagcctgcg ccagcacatc gtcatgctgc gcgcggagct cgagagcgcc
     5101 gtcgagcgcg gcacgtaacg gaggcgtttc ttgttcgcgt gcagctccct ggccctgcct
     5161 ccgaacgccg cgatccagcg cgacatcgcg cgtgccgccg aattccggcc cgccgatttc
     5221 atggacaagt tcgatgccca cacgctcttc tacgatttca tcgcgaccgg caaggagagg
     5281 gagcacagct ggctgctgat cgcgccgccg ctcctcaacc tcttgccgct gctggcccag
     5341 gcctcgatca acggtcagcg gatcggggcg ctcgtgacgc agtattacgc ccgctatctc
     5401 tgctgcgacg tctggatgat caacgtcagc agcgaaagcg ttctgtggaa gtttgccttc
     5461 ggcgcctgcc cgatcgaggc gcagccgtca cagaaccacc tctacgaagg aaaacgggtc
     5521 ctctatacgc tgtcgaagga caacgacctt cgctggatcg ccgactgggt ccgcttccat
     5581 gtgcgcaatc atggcgccga tgccgtcttg atctacgaca atgcgtcgac ccgctacagc
     5641 ggggaagatc tggaacaggc gctcagaagc gaaaacccct cgctcgaagt gaacgtcgtg
     5701 aactggccgt tcaaatacgg accgcaaggc atcgacgatc gatggtggga ctccaatttc
     5761 tgtcagtcgg gcgcccttca cgatgcgcgg ttccgcttcc tcgaacgcgc ccggagcgtc
     5821 ctcaattgcg acatcgacga gctggtggtg tcggagaccg ggggaagcgt tttcgcggcg
     5881 acggagaagg ccgatggcgg ctatttgctg ctcggcggca gatggatggc gagcacggcg
     5941 gagacgccgg cgggcgcgct gccaagacat gccgacttcc gctatctcga cgccgccgcg
     6001 gccgcttgtc cgaccaaatg gtgcgtcgtc cccgccaaat gtgccttgga gcgtcaatgg
     6061 cacacgcatg gcgtgggggc gaagccggtc gagctcaatc gcacgttctc ctaccggcat
     6121 tttcgcagca tatcgacgga ttggaagtat ccgaggacgc cattggcagc ctatgactcc
     6181 cgcatccatg agcgcgacgg gccactctcg gaggccatga ggcgggcggg actgtgatcg
     6241 cgaccgcggc ttctctctct cgcgaccgtt tccctggcgt gaagtttgag ctagatcagg
     6301 actcgaggtc gcattcgggc gtcgccgggc aaggcaacgg tatgatcgtc gccggcgaaa
     6361 gacaaaggga gctcccatga gaattcatct gatggcgatg acggcgctgc tggcgggtgc
     6421 ggcaagcgcg gccgtggccg aggagaagcc gatcaagatc gccttgatcg ccgacaagac
     6481 cgggccgctc gaggcctatg ccaagcagac ctcggccggc ctcgccatgg ggctccaata
     6541 cgccacggga ggcacgatga cggtggccgg ccacaagatc gaggtcatcg agaaggacag
     6601 ccagaccaag cccgatgtcg gccgcaccct gctgggcgaa gcctataacg atgacggcgc
     6661 cgatatcgcc atcggtggca ccagctcggc ggtggcgctg gcgatgctcc cggtggccga
     6721 ggacaacaag aagatcttca tcgtcgagcc ggcggtcgcc gattccatca ccggcgacaa
     6781 atggaaccgc tacatcttcc gcaccgggcg caactcgacc caggacgcgg tctcgaatgc
     6841 gctcgccgtc ggcaagcccg gcaccgtggt ggcgacgctg gcgccggact atgccttcgg
     6901 ccacgatttc gtcaccgctt tcggtacggc gcttgctccg accggcgcca agctcgtcta
     6961 tgaggaatac gcgccgccgg gcaccaccga cttcaccgcg ccctatcagc gcatcctcga
     7021 tgcgctggcc aaggagcaag ggcgcaagat catcttcatc aactgggcgg gtggcggcaa
     7081 tccgatgtcg gcgcttcggg cgctcgatcc cgagcggctc ggaatcgcgc tcgcgacggg
     7141 cagcaacatc ctgccggcgc tcgtcgccta caaggacttc ccgggcatgg aaggcgccgg
     7201 ctactattac tacgaaaatc ccaagaacgc ggtgaatgat tggctgatcg ccgagaaccg
     7261 gaagcgcttc gacggcgccc cgcccgactt cttcacctgc ggcggcttca tcgccggcga
     7321 ggccgtggtc gaggcgctga agcgcacgca gggctcgacg gatacggaga agctgatcac
     7381 ggctatggaa ggcatggcct tcgactcgcc caagggacgc gtcatgttcc gcaaggagga
     7441 ccaccagctc ctgcagccga tgtatgcctt caagatcaag gtcgacccca aagtgccctg
     7501 ggccattccc gagctcgtcc gcgagattcc catcagcgag atgaagatcc cgatcatgaa
     7561 ccaccgcccg tgagacagcg ggccgcgagc gggccgccat gacgggggaa cgcgcacccg
     7621 tcctcgcgac gagcgatctc accattcgct tcggcggaca gatcgcggtc aatggcgtct
     7681 cctgcgcctt cgcggagggc agcctgacgg cgatcgtcgg ccccaatggc gcgggcaaga
     7741 ccacctattt caatctcatc tcgggtcagc tgcggccatc ggccgggagc gtgcgcttcc
     7801 tcggccggga cgtcacccct ctcggcgcgc ccgagcgcac gcggctcggg ctcggccgct
     7861 cgtt
//
LOCUS       DASYNS010000329        22064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3981830, whole genome shotgun sequence.
ACCESSION   DASYNS010000329 DASYNS010000000
VERSION     DASYNS010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 22064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22064
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3981830"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..804
                     /locus_tag="VGP50_10150"
     CDS             103..804
                     /locus_tag="VGP50_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEV8027784.1"
                     /translation="MIHLYYWPTPNGHKITLFLEEADLPYELHGVNIGKGQQFEPDFL
                     KISPNNRMPAMTDDDPPGGGAPVSIFESGAILVYLAEKTGKFLPKDLRGRFEVLQWLF
                     WQMGGLGPMAGQNHHFSQYAPEKIAYAIDRYVKETSRLYAVLDKRLAGRAFIAGDYSI
                     ADMASYPWVVPYERQGQKLEDFANLKRWFEAIKARPATARAYERAKEFQTLPPNSEEA
                     RKVLFGQSAATVRGR"
     gene            complement(809..1777)
                     /locus_tag="VGP50_10155"
     CDS             complement(809..1777)
                     /locus_tag="VGP50_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV8027785.1"
                     /translation="MNFPTSLDENVIAAIAGILVFITVIIFAQALLPQDPMAGRLRSH
                     KKRRDALRGEMLGGGAVNNARRAAQISWVKGWLDKLKLLRGEEARKTSEQLSQAGWRS
                     RDALVIYVGLRLAMPFIGGIVTFVLLWVLWPDVPAMKKLMILLLGVIGIGFSPVFGLK
                     QAIKKRAKNLTKQLPDALDLLVICTEAGLSLDAAITRVGREMGFAAPHLADELGLTAV
                     ELGFLPNRRQALMNLAKRTNLPAIRGVTNTLIQTERYGTPLAHSLRVLSTEFREERML
                     KAEEKAHKLPAILTVPMIVFILPCLFVVLLGPAIIQVVATFGTGGK"
     gene            complement(1774..2748)
                     /locus_tag="VGP50_10160"
     CDS             complement(1774..2748)
                     /locus_tag="VGP50_10160"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV8027786.1"
                     /translation="MIELLTQPVVLIAIVGVGVALVLLFFAFADEGQRKQSKRVDRLR
                     TRGTEAANPDLQLRKDSGNKKTLDVMVRRLMPNPDQFRQKLYRTGKSITLGTYGIICA
                     VVAVVAILGSLIAGVSLLIAIPVGLLSGLWLPYLAIKYLGNRRAGQFMKLFPEAIGLM
                     VRGLKSGLPITETFQIVAQEISDPVGIEFRQVTDQIRLGHPLEQALLDAAKRVGTPEL
                     KFLTVTLSIQRETGGNLAETLANLDSVLRKRHTMKLKVKAMSSEARASAMIIGALPFI
                     MMALLSLINPGYIALLFTTTGGHKALAGGAFWMSTGIAVMAKMVRFEI"
     gene            complement(2745..4496)
                     /locus_tag="VGP50_10165"
     CDS             complement(2745..4496)
                     /locus_tag="VGP50_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEV8027787.1"
                     /translation="MFGKRDKEDSLQARLQALNARREQKPGAPAAAPPVRPPAPAAPP
                     AAERSPAPVATIGPKRIELSLEEARLLVDPAIAAAIDSGTARTKSRTELARKIDEVVR
                     ATLEEKKATLSALNQRDIVTTVLNSLVEKAQREAPQPPALTQAQSQIPVAAAAPPPAK
                     VSASRASIDQAIPRVYPLVMERIDPEVASKLQRAELARQLQAVVGEILIEQKIQLNQL
                     EQRDLVTVLLDDMLGLGPLEPLLSDESISDIMVNGPKQVYIERKGKLELTNVQFRDNG
                     HVMSIATRIVTAIGRRIDESTPLVDARLADGSRVNIIAPPLAIDGTSISIRKFSKKTI
                     NLDSMAKSGSISPEMAVVLKIAARARLNILISGGTGSGKTTTLNAMSQMIDEGERICT
                     IEDAAELQLQQPHVVRLETRPPNLEGKGEVTMRDLVKNTLRMRPDRIILGEIRGAEAL
                     DVLQAMNTGHDGSLATIHANRPREALTRLENLVGMSGVQLSPKAVRQQIAAAVHMILQ
                     ISRMRDGSRRIVSVTEVVGMEGEIITTQDLFTFQYDGENADGKLNGRFVSSGLRPHFT
                     PKAEYFGLDKALLEAMG"
     gene            complement(4496..5704)
                     /locus_tag="VGP50_10170"
     CDS             complement(4496..5704)
                     /locus_tag="VGP50_10170"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8027788.1"
                     /translation="MSEAAHMEPAASGSIRFVACVADDVTRETVSRAVAHLGWSNAKV
                     KAGGIEAALGIASQGSPPTLMLVDVTEASDPVNELAQLAETIGPDTTILAIGAVNDVS
                     LYRQLTAFGVADYLVKPVSSEVLCQALTASLRVYSAPGTARTTQLFAFIGARGGVGTT
                     TMALSTAWLLAHEFKLRTTLIDLDLHFGNLALSLDLEPGRGLREALEHPERTDSMLLA
                     AAMVSHAEKLPILATEEPLEEHLQFDGGAVAPLLTALAEDYDCLVVDLPRTLDPAARQ
                     VIAAADSTIIVTDLSLSALRDTHRLIDLAKSLEARAKPLIVANQVGASHRGEIGRPEF
                     ERGIGGPVDLVVPFDVKAAVAAAQSGKAMVAAAATSKATIEMRKFASRLAGREAEKSA
                     KTGFFSRFKR"
     gene            complement(5742..6437)
                     /locus_tag="VGP50_10175"
     CDS             complement(5742..6437)
                     /locus_tag="VGP50_10175"
                     /inference="COORDINATES: protein motif:HMM:NF021025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly lipoprotein"
                     /protein_id="HEV8027789.1"
                     /translation="MKNRLFSTLCLGFGLALAGCTPPTSEWSDVQAQKKLHIDFVRME
                     HPAAFAPGTAELAPGEVVELVSFLEQSQVDGDDHLYLQAAVDDSLAAQRIASLTRQLS
                     PRGIGASILPASAKDVPADHMLVVLERYVVTPPNCPDWTKPAYGAQHSNAMPSNWSCA
                     DTTNLGLMVADPRDLVVGRTLGPAEGNPATAGILRYRECKTKPISSVTASTAYSAAPQ
                     ASGGDDCDKMTSQ"
     gene            complement(6447..7955)
                     /locus_tag="VGP50_10180"
     CDS             complement(6447..7955)
                     /locus_tag="VGP50_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEV8027790.1"
                     /translation="MIRAIALILALAAFVASNARAEEHDAAGIVAANGTAIKIESGRG
                     VLVRLEHAATSIFVADPDVADVQVKSQSLLYLVAKKAGTTALFAVNAQDKVVLNVPIE
                     VNYNLTRIQEAIHELVPNAAISVSTIENSVVLDGTVYAASEADDILGVVKRFLPDIQV
                     GAARGGTTGDTKMIVNRIKVAAPNQINLRVRVAEVSRDLIKQFGFNWDAAIQKGNMVF
                     GLAGNNATALATNATTGATAATAAGGLFQTRSLSTDLKTTVNSLFGQAAVGNVDLSSV
                     IDALDNNGLATILAEPNLTAVSGEPASFLVGGEFPIPTAATSTLGIQTVEVSWKKFGV
                     SLNFVATIASGNRINLHVAPEVSQVSNTFAVNIGGIQLPSITTSRAETTVELASGQSI
                     AIAGLLQNDVTETLKNFPFLGDVPILGQLFRSDQFRRQETELVIIVTPYIVRPIAAAN
                     KLALPTDGYVAPSDGDMLSTGAEYKAQTLNKSSAPVTRSGSGLIGPVGFDLD"
     gene            complement(7962..8834)
                     /gene="cpaB"
                     /locus_tag="VGP50_10185"
     CDS             complement(7962..8834)
                     /gene="cpaB"
                     /locus_tag="VGP50_10185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEV8027791.1"
                     /translation="MSPRRIIFIVVALSIMGFTIFLVRGWLNAQRAELEAQRQQQPVV
                     QVQPSTMVLVAKGPLAAGQFLRPENLRWQAWPNDGIAPTYMVQGQHSLEEFIGAVVRS
                     SLSDGDPVTAARVVKPGDRGFLAVVLDPGHRAVTVNLTPSAGVAGFILPGDHVDLIAT
                     MTIPNVDTSKKGGGGSSRQNMASETVLKDVRVLALDQRLDDQNKEVVVAKTATLEVTP
                     KEAEIIAVVSDLGKLSLSLRSLATNVGDEVAAAQVAKGKLSFTYDSEATRLFPPPNAA
                     HGVLVVRGTTSSQE"
     gene            complement(8850..9353)
                     /locus_tag="VGP50_10190"
     CDS             complement(8850..9353)
                     /locus_tag="VGP50_10190"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV8027792.1"
                     /translation="MPYAASLIDWFALFVLTAALIAAALSDAVTYLIPNRYSAVIVLA
                     FFVYAIGKPVSFSLQGLAVGGLLFVVGAVLFARGMLGGGDVKLLAATGLWTGFDQIVL
                     LGVSTAIAACVIAVAQLTPLRRLLPAHPGGAPAGTDFRSKLRQPMPFGVAVALGGICV
                     ALARLVS"
     gene            complement(9469..9639)
                     /locus_tag="VGP50_10195"
     CDS             complement(9469..9639)
                     /locus_tag="VGP50_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEV8027793.1"
                     /translation="MRHFIQNLISDRSGATAIEYGLIAALISVVIIGAVTLVGTNLSS
                     VFNSIATSLKAA"
     gene            9930..10205
                     /locus_tag="VGP50_10200"
     CDS             9930..10205
                     /locus_tag="VGP50_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027794.1"
                     /translation="MFGGLAALLLAGAVLPPMATGSRAEDRVPGEDSDTVPRAERGGE
                     PQPLRSYTDEQGRTCRVYERRVVIDGGPATALATVCRDVSGRWVLSR"
     gene            10475..10669
                     /locus_tag="VGP50_10205"
     CDS             10475..10669
                     /locus_tag="VGP50_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027795.1"
                     /translation="MMKALITAFALLSFVAASTIPLVARAADPTTESTTPKKATKKAP
                     KKAAAHKKKTTTKMKKPNPT"
     gene            complement(10735..11970)
                     /locus_tag="VGP50_10210"
     CDS             complement(10735..11970)
                     /locus_tag="VGP50_10210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEV8027796.1"
                     /translation="MALALLSDARWLDKRRLRDYATMLIIVYVASALWALAGHGIDDP
                     LGHPVGTDFLSFWTVSSALHTGESHAIYAPLELAALERGLSGNDGFFAWAYPPIALLL
                     VYPLAFLPYLWSLAAWLAFGLGTYLSALWRILPRPLTLWAGLAFPAVFVNLSHGQNGL
                     LSTALLGWALLLLKERGALAGVLLGLLTFKPQLGLLIPFALIAGGHWRALAAAALTAL
                     ALTGLSLALFGTALWSDFVASLPFARAMLEQELVPYYKMQSIFAASRLVGAPAALAYT
                     LQGFVALSALWITISTWRRPVDPELKNAVLVTAGTLAAPFILDYDLMLLALPMAWLAC
                     RGLRSGFLPWEKITIAAVCVIPLTARMIAYGTDLALAPLAEMALLAVLMTRIRVERAA
                     PGRAPRPDEATGSVAPGIA"
     gene            12124..13629
                     /locus_tag="VGP50_10215"
     CDS             12124..13629
                     /locus_tag="VGP50_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027797.1"
                     /translation="MAISAGTETAPGEAGTGRAWHWPLLSWPLIAALVALARPLSDRM
                     ALLNDPDTYLHVAAGRWMIDHLALPFHDPFSHSLQGATWIPHEWLAEVALALAYGALG
                     WSGVVLLAMLCFALAMALLTRQLLAHFEPFSGLILAGLSSVLLLQHLLARPHVLALPL
                     LVAWPAALFAARDKGRVPSLLILPLMTLWANLHGSFMVGLALTGFVAAEAVLTTPGRA
                     CWTSLRQWAVFTLLAALAALVTPNGIDGVLLPFRFMGMATLQSSFNEWLSPNFQTFQP
                     LELWLLVTMFVGFASGVRLPLLRLVLLLGLFHLALAHARHADFVAIIAPLAIAASLGP
                     QLAARIHPTGPSPLSQRLMALARPANALGIAVAAAILVAISAAMLLRPIERPESPATP
                     VAAVAAAERLGLSGPVLNSEIFGGYLIFSGVPTFIDGRIEMYGDAFLARYVAIENGDG
                     ATLQDVLAADHITWTLLDPRQRAAQVMATLPGWRRVYDDAYAVIDVRATSP"
     gene            complement(13788..15032)
                     /locus_tag="VGP50_10220"
     CDS             complement(13788..15032)
                     /locus_tag="VGP50_10220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEV8027798.1"
                     /translation="MVPLSRQGLVVIGGTCVLFGLYGWAIFLTTFGHDGAIGPRYNAP
                     GADWVVFYSAAQAWLGGDVSLIFDGQRFTDYQNAAFAGWLSGPMPFAPWLYPPHFLLL
                     ILPFGLLPFAWSYAVFMAVTFAALLAAVWRHFAPDERRLQVISLLLCPATSITLVTGQ
                     NAFLSGALLFGGFSALDRYPVVAGAMLGALSFKPSLALMVPVALIAARQWRALAAAGA
                     TALVLALASLAVLGIEPWREWLLMMVSPSDDYRHWLDAGRSWGLSLYTCATLLGASAG
                     AANLVQMAATASAAAVVFWSFRQPLPADRRLVLLLAAAIFAAPHVSTYDTILLALATG
                     LLFRRVLVAPLSLPLAAVILAAWLLPLINPPRATPLGLATPLVVALVMVAAIAGARAK
                     APLIPSPFAGEAGPSHMLPRHR"
     gene            complement(15080..15442)
                     /locus_tag="VGP50_10225"
     CDS             complement(15080..15442)
                     /locus_tag="VGP50_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027799.1"
                     /translation="MIKHCTPAADERVFGTVTRLARSALPMLLLLGLARCGEWLPPFE
                     TVPRPAPPADTSGTIRVGVCYNFLTTTADEVRGIAAVSCGPTATPQPVERDLSLNYCP
                     LLVPSRATFTCVAPDNQQ"
     gene            15478..15846
                     /locus_tag="VGP50_10230"
     CDS             15478..15846
                     /locus_tag="VGP50_10230"
                     /inference="COORDINATES: protein motif:HMM:NF020457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HEV8027800.1"
                     /translation="MTEEFADTAGIEGYHAHVYYEPATRGVAERVRQALGERFPVRLG
                     RWHDVPVGPHPGAMYQVAFAVDAFPLLVPWLMLNRGGLSVLVHPLTGDDYEDHARFAL
                     WLGAPLPLRLEMLRRAPASD"
     gene            complement(16164..16628)
                     /locus_tag="VGP50_10235"
     CDS             complement(16164..16628)
                     /locus_tag="VGP50_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027801.1"
                     /translation="MSSSSIQARASSMSMATLGMAFVAGVLATLVFHQITLWILHVAG
                     IVPFAPFNMAPTKPFGVPDVVSLAFWGGVWGVLFQLTLARWFRGTTYWIAAIVAGGIF
                     PTLVFLFVVAPLKFGGVPSDVAGFFVIGFILNAMWGLGWALFQMLFRKMRAA"
     regulatory      16744..16862
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16947..18782
                     /gene="thiC"
                     /locus_tag="VGP50_10240"
     CDS             16947..18782
                     /gene="thiC"
                     /locus_tag="VGP50_10240"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEV8027802.1"
                     /translation="MPEGTVTGLTVSTGALPASRKIHVHGQRHAELRVALREIDLSPA
                     AKEPPVRVYDPSGPYTDPAIAIDIGAGLAGLRDDWIRARGDVEVRQGRSTRPEDNGLR
                     PGEVTSVPLFDRGRRAVLRAKAGGAATQLAYARAGIVTPEMEYVAIRETLGREGRADV
                     AARHDGESFGAAIPSHVTPEFVRDEVARGRAIIPANINHPESEPMIIGRNFLVKINAN
                     IGNSIITSSVAEEVEKMVWAIRWGADTVMDLSTGKNIHTIREWIIRNAPVPIGTVPIY
                     QALEKVGGRAEELSWEIFRDTLVEQAEQGVDYFTIHAGVRLPFIPLTARRVTGIVSRG
                     GSIMAKWCLAHHQENFLYTRFAEICEIMRAYDVSFSLGDGLRPGSIADANDEAQFAEL
                     KTLGELTKIAWRHDVQVMIEGPGHVPMHKIKENMDLQLEHCHEAPFYTLGPLATDIAP
                     GYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKTGVIAYKIAAHAADLAKG
                     HPAARARDDALSRARFEFRWRVQFNLSLDPETAEKFHDQTLPAEGAKLAHFCSMCGPK
                     FCSMKISQEVRDFAQSGMADKSAEFRQLGSQLYKVVEGRAAPIEP"
     gene            18779..19231
                     /locus_tag="VGP50_10245"
     CDS             18779..19231
                     /locus_tag="VGP50_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV8027803.1"
                     /translation="MKDVSSRLYPDRPMVGCIAVVRRGGRVLLAQRSVPPGVGFWGFP
                     GGLLELGETVHACARRELEEETGIIADPIATLGVIDRIAHDEERRVKTHFALVVVALE
                     WRSGEGEPIEDATALGWYTPEEVRTKRLELFPQAEALMAQALRWPTGS"
     gene            19380..19502
                     /locus_tag="VGP50_10250"
     CDS             19380..19502
                     /locus_tag="VGP50_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027804.1"
                     /translation="MRFGHGGFTSIDVPLGGSYKFYKQLSVGDFHRIYRGRSLL"
     gene            complement(19562..20401)
                     /locus_tag="VGP50_10255"
     CDS             complement(19562..20401)
                     /locus_tag="VGP50_10255"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8027805.1"
                     /translation="MSKVWFVTGAGRGLGIDIAQKALAAGHSVVATGRKPEAVLKAIG
                     AHDNLVTVALDITDPEAAKRAAKAAVDRFGRVDVLVNNAGNFYAGYFENISPAHFRAQ
                     IETNFFGPVNVTRAILPIMRAQRGGHVITVTSLAGLIGQEFVVAYAASKFALEGWMES
                     LRFDVAPFGIKTMNVEPGFFRTELLVEGSSTIWPDLKVDDYAERTKNTITAWSGMNGL
                     QGGDPAKLAAALVTLSDSGELPLRFIAGTDAMAAVEAKLKTIQEQINAHRDLSASLVI
                     DKC"
     gene            20552..21250
                     /locus_tag="VGP50_10260"
     CDS             20552..21250
                     /locus_tag="VGP50_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027806.1"
                     /translation="MIEEGRGLCVQSLDRTHIFIAQFEVENREVLNHPIFIHGFRDHY
                     DVSLDMPAKNDLRDGFAVLGSGRDQRVFLKDVTLPLCERSPALGLNLKLLMDHLSINS
                     LIEGIALDLIHCGRGLIVQSEIHNAVGIKIADADGLERAFLAKFLHRAPRSINIAVGL
                     VKEVEVEIVELEPLKRVLRGALCPLKCQAPCFLWGYGQSPPIAKGPAIPARSRTSAAH
                     IKMTSVAAIIRAER"
     gene            complement(21412..21819)
                     /locus_tag="VGP50_10265"
     CDS             complement(21412..21819)
                     /locus_tag="VGP50_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEV8027807.1"
                     /translation="MSERDFVDLFAIARAAPDPGVLLATLVGWKVAGLPGAAVASLAL
                     FVPSSILYYAIAKRWSDPRGQPWHSALEQGLAPVAAGLILGGSLLILRAGGGDPFLWA
                     IAAASTLVLLCRPRLNPLWVLAAGGLVAALAGA"
BASE COUNT         3877 a         6950 c         7579 g         3658 t
ORIGIN      
        1 gcggctcatc agagttgtcg ccgcgcgctt gccgcgcggg gcgggcgagg cgatagtggc
       61 cgggcccggc gcgtcccgcc gcacgacgga aggagaggaa acgtgatcca tctctattac
      121 tggcccaccc ccaacggcca caagatcacc ctctttctcg aagaggcgga tctcccctac
      181 gagctccacg gcgtcaatat cggcaagggc cagcagttcg agcccgattt cctcaagatc
      241 tcgccgaaca accgcatgcc ggcaatgacc gatgacgatc cgccgggcgg cggcgcaccg
      301 gtgagcattt tcgaatcggg cgccatcctc gtctatctgg cagagaagac cggcaaattc
      361 ctgcccaagg acctgcgcgg ccgcttcgag gtgctgcaat ggctgttctg gcagatgggc
      421 gggctcgggc cgatggccgg gcagaaccat catttcagcc aatatgcgcc ggagaagatc
      481 gcttacgcca tcgatcgcta tgtcaaggaa acgagccggc tctatgccgt gctcgacaag
      541 cggctcgccg gccgcgcctt catcgccggc gactactcga tcgccgacat ggcgagctat
      601 ccctgggtcg tgccctatga gcgccagggc cagaagctcg aggactttgc caatctgaag
      661 cgctggttcg aggcgatcaa ggcgcggccg gcaacggcgc gcgcctatga gcgggcgaag
      721 gagttccaga ctttgccgcc caacagcgag gaagcgcgca aagtcctgtt cggccagtcg
      781 gccgcgacgg tgcgcggccg ctgaagcgct atttgccgcc ggtgccgaag gtcgcgacga
      841 cctggatgat cgcggggccc agcagcacga cgaagagaca gggcagaatg aagacgatca
      901 tgggcacggt gaggatcgcc ggcagcttgt gagccttttc ctcggccttg agcatccgct
      961 cctcgcggaa ctcggtcgag agcacgcgca gcgaatgcgc gagcggcgtg ccgtagcgct
     1021 cggtctggat cagcgtgttg gtgacgccgc ggatggccgg cagattggtg cgcttcgcca
     1081 ggttcatgag cgcctggcgg cggttcggca ggaagcccag ctcgacggcg gtgaggccga
     1141 gttcgtcggc gagatgcggc gcggcgaagc ccatttcgcg ccccacgcgg gttatggccg
     1201 cgtcgaggct cagtccggcc tcggtgcaga tgaccaggag gtcgagcgcg tcgggaagct
     1261 gcttcgtcag atttttcgcc cgcttcttga tcgcctgctt gagcccgaac accggcgaga
     1321 agccgatgcc gatgacgccg aggagcagga tcatcagctt cttcatcgcc ggcacgtcgg
     1381 gccagaggac ccagagaaga acgaaggtga cgatgccgcc gatgaagggc atcgccaggc
     1441 gcaggccgac atagatgacg agggcgtcgc gcgagcgcca gccggcttgc gacagctgct
     1501 cgctggtctt gcgcgcttcc tcgccgcgca gcagcttcag cttgtcgagc cagcccttca
     1561 cccagctgat ctgcgccgcg cggcgcgcat tgttgacggc gccgcccccg agcatctcgc
     1621 cgcgcagggc gtcgcggcgc ttcttgtggc tgcgcagccg ccccgccatc gggtcttgcg
     1681 gcaagagcgc ctgggcgaag atgatcacgg tgatgaagac gaggatgccg gcgatcgcgg
     1741 cgatgacgtt ctcgtcgagg ctggtgggaa aattcatatc tcgaacctca ccatcttggc
     1801 catgacggcg atgccggtcg acatccagaa cgcgccgccc gccagcgcct tgtggccgcc
     1861 cgtcgtcgtg aagagcagcg cgatatagcc gggattgatg agcgacagca gcgccatcat
     1921 gatgaagggc agcgcgccga tgatcatggc gctggcgcgt gcctccgagg acatggcctt
     1981 gaccttgagc ttcatcgtgt ggcgcttgcg cagcacgcta tcgaggttgg cgagcgtctc
     2041 ggcgagattg ccgccggtct cgcgctggat cgagagcgtg acggtgagga atttgagctc
     2101 gggcgtgccg acgcgcttgg cggcgtcgag gagcgcctgc tcgagcgggt ggccgaggcg
     2161 gatctgatcg gtcacctggc ggaactcgat gccgacgggg tcggagattt cctgggcgac
     2221 gatctggaaa gtctcggtga tcggcaggcc cgatttgagg ccgcgcacca tgaggccgat
     2281 cgcctcgggg aagagcttca tgaactggcc ggcgcgccgg ttgccgagat atttgatggc
     2341 gagatagggc agccacaggc cgctcaggag cccgaccgga atggcgatca gcagcgacac
     2401 gccggcgatc agggagccga ggatggcgac gacggccacc accgcgcaga tgatgccgta
     2461 ggtgccgagc gtgatcgact tgccggtgcg gtagagcttc tggcggaatt ggtccgggtt
     2521 cggcatcaag cgccgcacca tcacgtcgag cgtctttttg ttgccgctgt ccttgcgcag
     2581 ctgcagatcg gggttcgccg cctcggtgcc gcgggtgcgc agccggtcga cgcgtttcga
     2641 ctgcttgcgc tggccctcgt cggcgaaagc gaagaagagg aggacgaggg cgacgccgac
     2701 gccgacgatg gcgatgagga cgacgggctg ggtgagaagc tcgatcatcc catcgcctcc
     2761 aggagtgcct tgtcgaggcc gaagtattcg gctttcggcg tgaaatgggg ccggagaccg
     2821 gacgagacga accgtccgtt gagcttcccg tccgcgttct cgccgtcgta ctggaaggtg
     2881 aagaggtcct gcgtggtgat gatctcgcct tccatgccca ccacctcggt gacggagacg
     2941 atgcggcgcg agccgtcgcg catgcggctt atctgcaaga tcatgtgaac ggcggcggcg
     3001 atctgctggc gcacggcctt gggcgagagc tgcacgcccg acatgccgac caggttctcg
     3061 agacgggtca gcgcctcgcg cggccggttg gcgtggatgg tggcgagcga gccgtcatgg
     3121 ccggtgttca tcgcctgcaa cacgtcgagc gcttcggcgc cgcggatctc accgaggatg
     3181 atgcggtcgg gacgcatacg cagcgtgttc ttgacgagat cgcgcatggt gacctcgccc
     3241 ttgccttcga ggttgggcgg gcgcgtctcc aggcgcacca cgtgcggctg ctggagctgc
     3301 aactcggccg cgtcctcgat ggtacagatg cgctcgccct cgtcgatcat ctgcgacatg
     3361 gcgttgaggg tcgtcgtctt accggaaccg gtaccgccgg agatgaggat gttgaggcgg
     3421 gcgcgcgcgg cgatcttgag caccaccgcc atttccggcg agatgctgcc gctcttcgcc
     3481 atggagtcga ggttgattgt cttcttggag aatttgcgga tcgagatcga ggtgccgtcg
     3541 atggccagcg gcggggcgat gatgttgacg cggctgccgt cggcgaggcg cgcgtcgacc
     3601 agcggcgtcg actcgtcgat gcgccggccg atcgccgtga cgatgcgggt ggcgatgctc
     3661 atcacatggc cgttgtcgcg gaactggacg ttggtcagct cgagcttgcc cttgcgctcg
     3721 atgtagacct gcttcggccc gttgaccatg atgtcgctga tcgactcgtc ggacagcagc
     3781 ggctcgaggg gcccgaggcc cagcatgtcg tcgagcagca cggtgacgag gtcgcgctgc
     3841 tcgagctggt tgagctggat cttctgctcg atcaggatct cgcccaccac cgcctggagc
     3901 tggcgggcga gctcggcgcg ctgcagcttc gaggcgactt cggggtcgat gcgctccatc
     3961 accagcggat agacgcgggg gatggcctga tcgatgctgg cgcggctggc gctgaccttc
     4021 gccggcggcg gtgcggccgc tgccaccggg atctgcgact gcgcctgggt tagcgccggc
     4081 ggctgcggcg cctcgcgctg cgccttctcg acgagcgagt tgagaacggt cgtgacgatg
     4141 tcgcgctgat tgagcgccga cagcgtcgcc ttcttctcct cgagcgtggc gcgcaccact
     4201 tcgtcgatct tgcgcgcgag ctcggtgcgc gacttggtgc gcgcggtgcc gctgtcgatg
     4261 gcggcggcga tcgccggatc gaccaggagg cgcgcctcct cgagcgacag ctcgatacgc
     4321 ttgggcccga tggtggcgac cggcgcgggc gaacgttcgg cggccggcgg cgccgcgggc
     4381 gcgggcgggc ggacaggcgg cgccgcggcg ggcgcgccag gcttctgctc acgccgcgcg
     4441 ttcagcgcct gaagacgcgc ctggagggag tcttccttgt cgcgttttcc gaacattagc
     4501 gtttgaacct gctgaagaat ccggttttcg ccgacttctc ggcctcgcgg ccggcgagac
     4561 gcgaggcgaa tttgcgcatc tcgatggtcg ccttgctggt ggcggcggcg gcgaccatcg
     4621 ccttgccgct ctgcgccgcg gcgacggcgg ccttgacgtc gaagggcacg acgaggtcta
     4681 ccgggccgcc gatgccgcgc tcgaattcgg gccggccgat ctcgccgcga tggctggcgc
     4741 ccacctggtt ggcgacgatc agcggcttgg cgcgcgcctc caaggatttc gcgagatcga
     4801 tcaggcggtg cgtgtcgcgc agcgccgaga gcgagagatc ggtgacgatg atcgtcgaat
     4861 ccgcagcagc gatcacctgg cgcgccgccg gatcgagcgt gcgcggcaag tcgacgacga
     4921 ggcagtcata gtcttcggcc agcgccgtga gcagcggcgc gaccgcgccg ccgtcgaatt
     4981 gcagatgctc ctcgagcggc tcttcggtgg cgaggatcgg cagtttctcg gcgtggctca
     5041 ccatggcggc ggcgagcagc atcgaatccg tgcgctccgg atgctcgagc gcctcgcgca
     5101 ggccgcgacc gggctcgaga tcgaggctca aggcgagatt gccgaaatgc aggtcgagat
     5161 cgatgagcgt ggtgcgcagc ttgaattcat gggcgaggag ccacgccgtc gaaagcgcca
     5221 tcgtcgtcgt gccgacgccg ccgcgcgcgc cgatgaaggc gaagagctgg gtggtgcgcg
     5281 ccgtgcccgg agcgctgtag acgcgcaacg acgcggtgag cgcctggcaa agcacctcgc
     5341 tcgacaccgg cttcaccagg tagtcggcga cgccgaaggc ggtcaactgg cgatagaggc
     5401 tgacgtcgtt gacggcgccg atcgccagga tcgtcgtgtc ggggccgatc gtctcggcga
     5461 gctgcgccag ctcgttcacg ggatcgctgg cttcggtgac gtcgacgagc atcagcgtgg
     5521 gcggggagcc ctggctggcg atgccgagcg cggcctcgat gccgccggct ttcaccttcg
     5581 cgttcgacca cccgagatgc gcgacggcgc gactcaccgt ctcgcgcgtc acgtcgtcgg
     5641 cgacgcaggc gacgaagcgg atgctgccac tggcagcggg ctccatgtgt gccgcttcgc
     5701 tcattatccg acctgctgct tcaaccatta caccactgct atcactgaga ggtcatcttg
     5761 tcgcaatcgt cgccgccgct ggcttgcgga gctgccgaat aggcggtgga cgcggtcacg
     5821 gacgagattg gtttcgtctt gcactcgcgg taacgcaaga tccccgcggt tgccgggttg
     5881 ccttccgccg ggccgagcgt ccgaccgacg accaggtcgc gcggatcggc gaccatgagg
     5941 ccgagattcg tcgtgtcggc gcagctccag ttacttggca ttgcgttgct gtgctgggcg
     6001 ccataagcgg gcttcgtcca atcgggacag ttgggcggag tcacgacgta gcgctcgagc
     6061 acgacgagca tgtgatcggc cggcacatcc ttcgccgagg ctggaaggat gctggcgcca
     6121 atgccgcggg gggagagctg acgggtcagg ctggcgatgc gctgcgccgc caggctgtcg
     6181 tcgaccgcgg cctggagata gagatgatcg tcgccgtcga cttgcgactg ctcgaggaac
     6241 gacaccagct cgacgacctc gcccggagcg agctcggcgg tgccgggcgc gaaggccgca
     6301 gggtgctcca tccgcacaaa gtcgatgtgc agctttttct gggcctgaac gtccgaccat
     6361 tcggaggtgg gcggcgtgca accggcgagc gcgaggccga agccgaggca gagcgtggaa
     6421 aaaaggcggt ttttcatgtg cagtcctcag tcgagatcga agccgacggg gccgatcaag
     6481 ccggacccgg agcgggtgac gggggcggaa ctcttgttca gcgtctgagc cttgtactcc
     6541 gcaccggtag acagcatgtc gccatcggac ggagcgacat agccgtcggt cgggagcgcc
     6601 agcttgttgg cagccgcgat ggggcggacg atatagggcg tcacgatgat gacgagctcc
     6661 gtctcctggc ggcggaactg atccgagcgg aagagctgcc cgaggatcgg cacgtcgccg
     6721 aggaagggaa agttcttgag cgtctcggtc acgtcgttct gcaagaggcc ggcgatggcg
     6781 atgctctggc cgctggccag ctcgacggtg gtttcggcgc gcgacgtcgt gatcgagggc
     6841 agctgaatgc cgccgatgtt gacggcaaag gtgttcgaga cctggctcac ctcaggcgcg
     6901 acgtgaaggt tgatgcggtt gccggacgcg atcgtcgcga cgaaattcaa gctgacgccg
     6961 aacttcttcc agctcacctc gaccgtctgg atgcccaggg tggaggtggc ggccgtcggg
     7021 atcgggaact cgccgccgac gaggaagctc gcgggctcgc ccgaaacggc ggtgagattg
     7081 ggctcggcca ggatcgtggc gaggccgttg ttgtcgagag catcgatcac cgagctcaga
     7141 tcgacgttgc cgaccgcggc ttggccgaag aggctgttca ccgtggtctt gaggtcggtc
     7201 gagagcgagc gcgtctggaa caggccgcct gcggcggtgg cagcggtcgc gccggtggtc
     7261 gcgttggtag ccagcgcggt ggcgttgttg ccggcaaggc cgaagaccat gttgcccttc
     7321 tgtatcgcgg cgtcccagtt gaagccgaac tgcttgatga ggtcgcgcga gacctcggcg
     7381 acgcggacgc gcagattgat ctggttgggc gccgcgacct tgatccggtt gacgatcatc
     7441 ttggtgtcgc ccgtcgtacc tccgcgtgca gccccgacct ggatgtcggg caggaagcgc
     7501 ttgacgactc ccaggatgtc atcggcctcc gaggccgcgt agaccgtgcc gtcgagcacc
     7561 acggagttct cgatcgtgct gaccgagatc gcggcgttcg gcaccagttc gtggatcgct
     7621 tcctggatgc gcgtcaggtt gtagttgacc tcgatgggga cgttgagcac gaccttgtcc
     7681 tgcgcattga cggcaaagag ggcggtcgtg ccggccttct tggccaccag gtagagcagc
     7741 gactgcgact tcacctgcac atcggcgacg tcgggatcgg cgacgaagat gctggtggcc
     7801 gcgtgctcga ggcgcacgag cacgcctcga ccgctctcga tcttgatcgc agtaccgttc
     7861 gcggcaacaa tgccggccgc atcgtgttct tcggcgcgcg cattcgatgc cacgaaggcg
     7921 gcgagcgcga gtatgagggc gatagcgcgg atcatcgtcg tctattcctg cgaagaggtg
     7981 gtgccgcgca cgacaagcac accatgcgct gcgttcggcg gtgggaagag ccttgttgct
     8041 tcgctgtcgt aggtgaagct gagcttgcct ttggcgacct gcgcggcagc gacctcgtcg
     8101 ccgacattgg tggcgaggct gcgcaggctc agcgagagct tgcccaggtc gctcaccacc
     8161 gcgatgatct cggcttcctt gggagtcact tcgagagtgg cggtcttggc gaccacgact
     8221 tccttgttct gatcgtcgag gcgctggtcg agggcgagca cacgcacgtc cttgagcacg
     8281 gtctcgctcg ccatgttctg ccgcgagctg ccgccgccgc ccttcttgct ggtgtcgacg
     8341 ttggggatcg tcatcgtcgc aatgaggtcg acatggtcgc cgggcaggat gaagccggcg
     8401 acgccggcgc tcggcgtcag gttgaccgtg acggcgcgat ggccgggatc gagaaccacg
     8461 gcgaggaagc cgcgatcgcc cggcttcacc acgcgcgcgg ccgtgacggg atcgccgtcg
     8521 ctcaggctgc tgcgcacgac ggcgccgatg aactcctcga gcgaatgctg gccctgcacc
     8581 atgtaggtcg gcgcgatgcc gtcattgggc caggcctgcc agcgcagatt ctcgggccgg
     8641 aggaactggc ctgccgcgag cgggcctttg gcgacgagca ccatcgtcga gggctgcacc
     8701 tggacgacgg gctgttgctg gcgctgcgct tcgagctcgg cgcgctgggc attgagccac
     8761 cctctcacca agaagatggt aaagcccatg atcgacaggg caacgacgat gaagatgatc
     8821 cggcggggcg acatgagaag ctccgatgat tacgagacga gtcgcgcgag cgcgacgcag
     8881 atgccgccga gagcgacggc aacgccgaaa ggcatcggct gacgcaattt gctgcggaag
     8941 tcggtgccgg cgggagcgcc gcccggatgg gcgggcagaa gacggcgtag cggcgtgagc
     9001 tgcgccaccg cgatgacgca ggcggcgatg gcggtgctga cacccagcag cacgatctgg
     9061 tcgaacccgg tccagagacc cgtggccgcc agaagcttga cgtcgccgcc gccgagcatg
     9121 ccgcgtgcga aaaggaccgc accgacgaca aacaagagcc cgccgacggc gaggccctgc
     9181 agcgagaagg agaccggctt gccgatggcg tagacgaaaa aggcgaggac gatgaccgcg
     9241 gaatagcggt ttggaatgag gtaggtcacc gcgtcgctca gggctgcggc aatgagcgcg
     9301 gccgtgagga cgaagagagc gaaccaatcg atcaggcttg cggcataagg catgaggttc
     9361 tccgtcaccc cgccgcgagc agcttcccgc tgccttctcg cccgaccgaa acgggagccg
     9421 cccgagggcg gctcccggtc agtcgagatt gtcgcgacga aggcgcgatt aggcggcctt
     9481 caagctggtc gcgatcgagt tgaagacgct gctgaggttc gtaccaacca gcgtcaccgc
     9541 accgatgatc acgacggaga tgagcgcggc gatcaggccg tactcgatcg cagtcgcacc
     9601 agagcgatcg ctgatgaggt tctggataaa gtggcgcata agagtcatgc ctcctggtca
     9661 agtgagcgac acggcaattc tggtatctac gtgttaatgt accattaata gccaagatga
     9721 gatccataca aggcgcgtca ttacatgatt aattcggctt taacgccagt ctttattaag
     9781 aggatattgc gaaaacgtgc gagaaactta attaaaattg agatgattgg ccgaattgtc
     9841 ccggaaccgg aaaccggcat tcgggtttgc tcaggcgaaa cagggtgagg gaagcaggta
     9901 aagatatggc gaaggcggca ggaaggcggg tgttcggagg attggcggcg ctgctgctcg
     9961 caggcgccgt gcttccgccc atggccactg gcagccgcgc cgaggatcgc gtgccaggcg
    10021 aggacagcga cacggtgccg cgcgccgagc ggggtggcga gccccaaccg ctgcgctcct
    10081 acaccgacga gcaaggacgg acctgccgcg tctatgagcg gcgcgtcgtc atcgatggcg
    10141 gaccggccac ggcgctcgcc accgtgtgcc gcgacgtgag cgggcgttgg gttctgtcgc
    10201 ggtgactggg gaagcgcgca gttccttgcg tcattttgcc gcataaactc cgcgatttct
    10261 tgtcccttct ttgcctcatc taggcaggcg caaatgagcg ctaggcatca tcgcatctcg
    10321 acaggaaaaa agccgcgttc tctttgctca aatcttccaa agccgtgggg aaccatgcta
    10381 gggtgcgcgc cgtccgttgc cggtcgtttc ctcgtctcgc aatccgacac ctcaccctaa
    10441 ccggtgcaaa gcaaatccca gaacccagga gcttgtgatg aaggcgctca ttaccgcatt
    10501 tgccctgctg tctttcgtcg cggcgtcgac cataccgctg gtcgcacgcg cggccgatcc
    10561 gaccaccgaa agcacgacac cgaagaaggc cacgaagaaa gcgccgaaga aggccgcggc
    10621 gcacaagaag aagaccacca ccaagatgaa gaagccgaat ccgacctgat tcgcgttgcc
    10681 agcaagtaac gaggcctccg gggagaccgc ttcccggagg ccttttcgtt gtggtcaggc
    10741 gattcccggc gcgacgctgc ccgtggcttc gtccgggcgc ggcgcccggc caggcgccgc
    10801 ccgctcgacc cggatccgcg tcatcagcac ggccagcagc gccatctcgg ccaaaggagc
    10861 gagcgcgaga tcggtgccat aggcaatcat gcgcgccgtc agtgggatga cgcagacggc
    10921 cgcgatggtg atcttctccc agggcaggaa gccgctccgc aagccgcgac aagcgagcca
    10981 cgccatcggc agcgccagca gcatgaggtc gtaatcgagg atgaagggcg cggcgagcgt
    11041 gccggcggtc accagcacgg cgttcttgag ctcgggatcg accggccggc gccaggtcga
    11101 gatggtgatc cagagagcgc tgagggcaac gaagccttga agcgtataag cgagcgcggc
    11161 cggagcgccg acgaggcgcg aagcggcaaa gatcgactgc atcttgtaat agggcaccag
    11221 ctcctgctcc agcatcgcgc gcgcgaaggg gaggctcgcc acgaaatccg accagagcgc
    11281 ggtgccgaag agggcgagcg acagtcccgt cagggccagc gccgtcaatg ccgccgcggc
    11341 gagggcgcgc caatgcccgc cggcgatgag cgcgaacggg atcaacaggc cgagctgcgg
    11401 cttgaaggtg agaagaccga gcagcacgcc ggccagcgcc ccgcgctcct tcagcagcag
    11461 cagcgcccag ccgagaagcg cggtgctgag cagcccgttc tgcccatggc tcagattgac
    11521 gaacaccgcc gggaaggcga ggcccgccca cagcgtgagc ggccgcggca ggatgcgcca
    11581 gagcgcgctc agataggtgc cgagcccaaa ggcgagccat gccgccagcg accatagata
    11641 gggcaagaac gccagcggat agacgagaag caaggcgatg ggtggatagg cccaggcgaa
    11701 aaaaccgtcg ttgccgctca ggccgcgctc caaggcagcg agctcgagcg gcgcgtagat
    11761 cgcgtggctc tcgcctgtgt ggagcgccga ggagacggtc cagaagctca ggaaatcggt
    11821 gccgacggga tgtccgagcg gatcgtcgat gccatggccc gcgagcgccc agagcgcgga
    11881 cgcgacatag acgatgatca gcatcgtcgc gtagtcgcgc aggcgccgct tgtccaacca
    11941 tcgcgcatcg ctgagcagcg ccagagccaa gagcacctcc gcagattgcc ctggactatc
    12001 ggcgatggtt ctttagcgaa tgatttattt cgccgcgggc ttcagccggc gggcggcgcg
    12061 taaggacttg taaaggatgc gccgcaacaa tccacccgac accgatcgcg agacttccag
    12121 cacatggcga tatccgccgg gacggagacg gcgccgggag aggcgggcac ggggcgcgcc
    12181 tggcactggc cgctgctgtc ctggccgctc atcgccgcgc tggtcgcgct ggcgcgcccg
    12241 ctcagcgatc ggatggcgct cctcaacgat cccgacacct atctgcacgt cgccgccggg
    12301 cgctggatga tcgatcatct ggcgctgccc tttcatgatc ctttctcgca ttcgctccag
    12361 ggcgcgacgt ggatccccca tgagtggctg gcggaagttg cgctcgcgct cgcttacggc
    12421 gcgctcggct ggagcggcgt cgtgctcctg gcgatgctgt gctttgcctt ggccatggcg
    12481 ctgctgacgc ggcagctcct cgctcatttc gagcccttct ccggcctgat cctcgccggg
    12541 ctgagcagcg tgctgctgct gcagcatctg ctggcgcgcc cgcatgtcct ggcgctgccg
    12601 ctgctcgtgg cctggcccgc ggcgctcttc gcggcgcgcg acaagggacg cgtgccctcg
    12661 ctgctgatcc tgccgctcat gacgctctgg gccaatcttc acggtagctt catggtcggc
    12721 ctggccctga ccggcttcgt cgccgccgag gcggtgctga cgacgcccgg ccgggcgtgc
    12781 tggacgagcc tccggcaatg ggccgtgttc acgcttctcg ccgcgctcgc ggcgcttgtc
    12841 acgcccaacg gcatcgacgg cgtgctgctg cccttccgct tcatgggcat ggcgacgctg
    12901 caaagcagct tcaacgagtg gctgagcccg aatttccaga ccttccagcc gctcgagctc
    12961 tggctcctcg tcacgatgtt cgtgggcttc gcttcgggcg tgcgcctgcc gctgctgcgg
    13021 ctcgtgctgc tgctcggact cttccatctg gcgctggcgc atgcgcggca cgccgacttc
    13081 gtggcgatca tcgcgccgct cgcgatagcg gcatcgctcg ggccgcagct cgccgcgcgc
    13141 atccacccga ccggcccgtc accgctgagc cagcgcctca tggctctggc gcggccggcg
    13201 aatgcgctcg gcatcgccgt cgctgccgcg atcctcgtcg cgatcagcgc cgcgatgctg
    13261 ctgcggccga tcgagcgccc cgaaagcccg gccacgcccg tcgctgccgt cgccgcggca
    13321 gagcgcctgg gcttgtccgg gccggtgctc aacagcgaga tcttcggcgg ctatctcatt
    13381 ttcagcggcg tgcccacctt catcgacggg cgcatcgaga tgtatggcga cgccttcctc
    13441 gcgcgctatg tcgcgatcga gaatggcgac ggggcgacgc tgcaggatgt gctcgccgcg
    13501 gatcacatca cctggacctt gctcgatccg cggcagcgcg cggcgcaggt gatggcgacc
    13561 ttgccgggct ggcggcgcgt ctacgacgac gcttatgcgg tgatcgacgt ccgggcaact
    13621 tcgccctgaa gcggggcgcg cgagtcccgc ttctccctct ccccacaggg ctatggcaga
    13681 tggggttcac cacggaggca cggaggacac ggagaagaga ggttgggcgc gcgaagcgcg
    13741 ccatcttcgc ttctccgtgc tctccgtgcc tctgtgttta atctttccta tcggtgtctc
    13801 ggcagcatat gcgacggccc tgcctctccc gcaaaaggag aggggataag gggcgccttg
    13861 gcgcgcgcgc cggcgatcgc cgcgaccatg accagagcga cgacgagcgg ggtcgccaag
    13921 ccgagtggcg tggcgcgcgg cggattgatc aacggcaaga gccaggcggc gaggatcacg
    13981 gcggccagcg gcaaggacaa cggcgccacg agcacgcggc ggaagagcag gccggttgcc
    14041 agcgcgagga ggatggtgtc gtaggtcgag acatggggcg ccgcgaagat tgccgccgcg
    14101 agcagcagga cgagccggcg atccgccggg agcggctggc gaaagctcca gaagacgacc
    14161 gctgccgccg acgctgtcgc ggccatctgg acgagattgg cggcaccggc ggaggcgccc
    14221 agcagcgtgg cgcaggtgta gaggctcaag ccccaggagc ggccggcgtc gagccagtgg
    14281 cgataatcgt ccgacgggct caccatcatc aggagccatt cccgccaggg ctcgataccg
    14341 aggacggcga ggctcgcgag cgcgagaacc agcgccgtcg cgccggcagc ggcgagcgcc
    14401 cgccactgcc gcgcggcgat caaagccacg ggaaccatga gcgcgagcga cggcttgaag
    14461 ctgagcgcgc ccagcatcgc gccggcgacg acgggatacc ggtccaaggc cgagaacccg
    14521 ccgaacagca gcgcgcccga gaggaaggcg ttctgtccgg tcaccagcgt gatggaggtg
    14581 gcggggcaga gcagcagcga gatcacctga aggcgccgct cgtccggcgc gaaatgccgc
    14641 cagaccgcag cgagcagcgc ggcgaacgtc accgccatga acaccgcgta ggaccaggcg
    14701 aagggaagta gcccgaaagg caggatcagc agcaggaaat gcggcggata gagccagggc
    14761 gcgaagggca tggggcccga cagccaaccg gcgaaggcgg cgttctggta gtcggtgaaa
    14821 cgctgcccgt cgaagatgag cgagacatcg ccgccgagcc aggcctgtgc cgcgctgtag
    14881 aagacgaccc aatcggcgcc cggcgcgttg tagcgggggc cgatggcgcc gtcatggccg
    14941 aaggtcgtga ggaagatggc ccagccatag agcccgaaga gcacgcaagt gccgccgatg
    15001 acgaccaagc cctgccgcga cagcggtacc atgggatgct ccgagcgcgc gaacgcggct
    15061 catcctgcgg cagagccctt tattgttggt tatcgggcgc gacgcaggtg aaggtggcgc
    15121 ggctcggaac caggagcggg cagtaattga ggctgaggtc gcgctcgacc ggctgcggcg
    15181 tcgccgtcgg gccgcagctc acggcggcga tgccgcgcac ttcgtcggcg gtggtggtga
    15241 ggaagttgta gcagacgccg acacggatcg tgccgctcgt gtccgccggc ggggctggcc
    15301 gcggcaccgt ctcgaatggc ggcagccatt cgccgcaacg ggcgaggccg agcagcagca
    15361 gcatgggcag ggcggagcgg gcgaggcgcg tcacggtgcc gaagactcgc tcatcggcgg
    15421 ccggcgtgca atgcttgatc acggggcttg atcgcgggcc gatgcatggg gcgcggaatg
    15481 accgaggagt ttgccgatac cgcggggatc gagggctatc acgcccatgt ctattacgag
    15541 ccggcgacgc ggggcgtggc ggaacgcgtg cgccaggccc ttggcgagcg cttcccggtg
    15601 cggcttggcc gctggcacga cgtgccggtc gggccgcatc cgggcgcgat gtatcaagtc
    15661 gcattcgctg tcgacgcgtt cccgcttctc gtgccgtggc tcatgctcaa ccgcggcggc
    15721 ctcagcgtcc tcgtccatcc cctgacgggc gatgattacg aggatcacgc gcgctttgcg
    15781 ctctggctcg gcgcgcccct gccgctgcgc ctcgaaatgc tccgccgcgc tccggcttcg
    15841 gactaggtcg cgtggacatt cgcagtgatg cgcgtctctg cccctctccc gcatcgcggg
    15901 gtggcgagcg cggagcgcgc gcgccgggtt aggggacggt tcaagcaaat gcgctttgcc
    15961 tgacaggtcc cctcaccctc ccactgcttc gctgaagctt cgcagtgggt ccctccctct
    16021 cccgtgatgc gggagagggg atgtttggcg gatgtttagg cacgaatgtc cacagcgcct
    16081 aggcagcgtt gccttgatcg cacaacgcaa tcccctctcc ccgaggggag aggggattgg
    16141 cgcggcggag gggacggacg gcgtcaggcc gcgcgcatct tccggaacag catctggaag
    16201 agcgcccagc ccaaccccca catggcgttc agaatgaagc cgatgacgaa gaacccggcg
    16261 acgtcggacg ggacgccgcc gaatttgagc ggggcgacga cgaagaggaa gaccagcgtc
    16321 gggaagatgc cgccggcgac gatcgccgcg atccaatacg tggtccccct gaaccagcgc
    16381 gccagagtca gctgaaagag caccccccag acgccgcccc aaaacgccag cgagacgacg
    16441 tcggggacgc cgaagggctt ggtcggcgcc atgttgaagg gcgcgaacgg gacgatgccc
    16501 gcaacatgca ggatccacaa ggtgatctgg tgaaaaacca gcgtcgcgag gacgccggcc
    16561 acgaacgcca tgccgagcgt ggccatcgac atcgacgacg cgcgcgcctg aatggacgat
    16621 gaactcatct ggcttctccc cctgttcttt cggctgcggc gctatcgccg cggccaccat
    16681 cctgcgcgcg tcgggcaaga gcggcaagcg gcttgacggc ggccggcatg ggtcacgata
    16741 ctgaagcatt ccaaggggag ccccggtaag gggctgagat accacatagc ctcgggcgac
    16801 gtgggaaccc tttgaacctg atccgggtcg taccggcgta gggacaggaa cagcgcggct
    16861 cgaagctttt gggcgagagc cgcccctcaa ctccgcaggc accggatctc ctgttgcacc
    16921 cgatggttcg ggaggaggag atcaccatgc cagaaggcac tgtcacaggt ctgacggtct
    16981 cgacgggcgc tctgccggcg tcgcggaaga ttcacgtcca cgggcagcgt catgccgagc
    17041 tgcgcgtcgc gctccgcgag atcgatctct cgccggcggc gaaggagccg ccggtccggg
    17101 tctacgatcc gtcgggaccc tataccgatc ccgctatcgc catcgatatc ggcgccgggc
    17161 tcgcaggcct gcgcgacgac tggatccgcg cacgcggcga cgtcgaggtg cgccagggcc
    17221 gctcgacgcg ccccgaggat aacgggctca ggccgggcga ggtcacgagc gtgccgctgt
    17281 tcgatcgcgg ccggcgcgcc gtgctgcggg cgaaggccgg cggcgccgcg acgcagctcg
    17341 cttatgcccg cgccggcatc gtcacgcccg agatggagta tgtcgcgatc cgcgagactc
    17401 tggggcgcga agggcgcgcg gacgtggcgg cgcggcacga cggcgaatcc ttcggcgcgg
    17461 cgatcccgag ccatgtcacg cccgaattcg tgcgcgacga agtggcgcgc ggccgcgcca
    17521 tcatccccgc caacatcaac catccggaaa gcgagccgat gatcatcggc cgcaatttcc
    17581 tggtgaagat caacgccaat atcggcaact cgatcatcac gtcgtcggtg gcggaagagg
    17641 tcgagaagat ggtgtgggcg atccgctggg gcgccgacac ggtgatggat ctctcgacgg
    17701 gcaagaacat ccacaccatc cgcgaatgga tcatccgcaa cgcgccggtg ccgatcggca
    17761 cggtgccgat ctatcaggcg ctcgagaagg tcggcggcag ggccgaggag ctgagctggg
    17821 agatcttccg cgacacgctc gtcgagcagg cggagcaggg cgttgattat ttcaccatcc
    17881 atgccggcgt gcggctgccc ttcatcccgc tcaccgcgcg gcgcgtcacc ggcatcgtct
    17941 cgcgcggcgg ctcgatcatg gcgaaatggt gcctcgcgca tcaccaggag aatttcctct
    18001 acacgcgctt tgccgagatc tgcgagatca tgcgcgccta tgacgtgagc ttctcgctgg
    18061 gcgacggctt gcgccccggc tcgatcgccg acgccaatga cgaggcgcaa ttcgccgagc
    18121 tcaagactct gggcgagctc acgaaaattg cgtggcggca cgacgtgcag gtgatgatcg
    18181 aagggccggg ccatgtgccc atgcacaaga tcaaggagaa catggatctc cagctcgagc
    18241 attgccacga ggcgccgttc tacacgctgg ggccgctcgc caccgacatc gcgccgggct
    18301 acgaccacat cacctcggcg atcggcgcgg cgatgatcgg ctggttcggc acggcgatgc
    18361 tctgctacgt cacgcccaag gagcatctgg ggttgccgga ccgcgacgac gtcaagacgg
    18421 gggtcatcgc ctacaagatc gcggcccatg ccgccgacct cgccaagggc catcccgccg
    18481 cgcgcgcgcg cgacgacgcg ctctcgcgcg cccgcttcga gttccgctgg cgcgtccagt
    18541 tcaacctctc gctcgacccc gagacggcgg agaagttcca cgaccagacg ctccccgccg
    18601 agggcgccaa gctcgcgcat ttctgctcga tgtgcggccc caaattctgc tcgatgaaga
    18661 tctcgcagga ggtgcgcgac ttcgcgcagt cgggcatggc cgacaaatcg gcggagttcc
    18721 gccagctcgg cagccagctc tacaaagtgg tcgaaggacg ggcggcgccg atcgagccgt
    18781 gaaggacgtc tcgagccggc tctatcccga ccggccgatg gtcggctgca tcgccgtggt
    18841 gcggcgcggc ggccgcgtgc tgctggcgca gcgctcggta ccgccaggcg tcggattctg
    18901 gggttttccg ggcggcctcc tcgagctcgg cgagacggtc catgcctgcg ccaggcgcga
    18961 gcttgaggaa gaaacgggca tcatcgccga tcccatcgcc acgctcggcg tcatcgaccg
    19021 catcgcccac gatgaagagc ggcgcgtaaa gacacatttc gcgctcgtgg tggtggcgct
    19081 cgaatggcgc tcgggcgagg gcgagccgat cgaggacgcg accgcgctcg gctggtacac
    19141 gccggaggag gtgaggacga agcggctcga gcttttcccc caggcggagg cgctgatggc
    19201 gcaggcgcta cggtggccaa ctggttcatg agtcgatcga gaatcgaccg tgtccggtcg
    19261 gccggccatg ttcgtctgaa gctcggtgct cacggtcaga agacggaatc acccccgatc
    19321 gctctcttca actgactttc gcaagactat ctgccaacgc cagcataaat gcagaaatag
    19381 tgcgtttcgg acatggcggc ttcacctcga tcgatgtccc tctgggcggc agttacaagt
    19441 tctacaagca actctcggtc ggcgatttcc atcgcatcta ccgcggccgg tctttgctgt
    19501 gaccaaacta gaacgcgtag agggaagtcc agtcccccaa actgttgctg ggttaaccgc
    19561 tttagcattt atcgatcacg agcgatgcag acaggtcgcg atgtgcgttg atctgttcct
    19621 gaatggtctt gagcttggcc tcgactgctg ccatcgcgtc tgtgccggca ataaatcgga
    19681 gcgggagttc gccggaatca gacagggtaa ccaatgctgc ggcgagcttg gcggggtcgc
    19741 cgccctgcaa gccgttcatg cccgaccacg ctgtgatagt gttcttcgtg cgctcggcat
    19801 aatcgtcaac cttcaggtcc ggccatatcg tcgaggaacc ttcgaccaaa agttcggttc
    19861 ggaagaagcc gggttcgacg ttcattgtct tgatcccaaa gggcgcaacg tcgaagcgaa
    19921 gcgactccat ccatccctca agcgcgaact tcgacgcggc ataggcaacg acgaactcct
    19981 gaccgatcag accggcgagc gaggtgacgg taatcacgtg gccaccgcgt tgcgctcgca
    20041 tgatggggag aattgcgcgg gtgacgttca cgggaccgaa aaaattagtc tctatctgcg
    20101 cccggaaatg cgcggggctg atgttctcga aatagcccgc gtagaagttg cccgcattat
    20161 tcacgagaac gtcgacccgg ccaaagcggt ctaccgcagc cttggcggct ctcttcgcag
    20221 cttctggatc ggtgatgtcg agtgcgaccg tcaccaggtt atcatgagcg ccgatggctt
    20281 tcaggacggc ttccggtttg cggcctgttg ccactaccga atgcccggct gcgagcgcct
    20341 tttgcgcgat gtcgatgcca agcccacggc cagcgccggt gacgaaccaa actttgctca
    20401 ttgcatcctc tatcccgacg aaacaagaat tctcgggaag cggacctctt cgcctgaggc
    20461 cgctcccgca tcaaacaggg cggaagaacc catacgtcac ttagacatcc agtctgcgcg
    20521 tgctcagttt ctcgacccat tcaggatcgc ggtgatcgaa gaaggccgtg gtctttgtgt
    20581 ccagagcctt gatcgcactc atatcttcat tgctcagttc gaagtcgaaa accgtgaagt
    20641 tctgaatcat cctatcttta ttcacggatt tcgggatcac tacgatgttt ctttggatat
    20701 gccagcgaag aatgacctgc gcgacggatt tgccgtactt ggctccggta gagaccaacg
    20761 tgtctttctt aaagatgtca cgctgcccct ctgcgaaagg agcccagccc tcggtttgaa
    20821 tcttaaattg ctcatggatc acttgagcat caattcgctg attgaaggga ttgccctcga
    20881 tctgattcac tgcgggcggg gccttattgt gcagagcgaa atccacaacg cggtcggtat
    20941 aaaaattgct gacgccgatg gcctagagcg tgccttcttg gcgaagttcc tccatcgcgc
    21001 gccacgctcc ataaacatcg ccgtagggct ggtgaaggag gtagaggtcg agatagtcga
    21061 gctggagcct cttaagcgag ttcttcgcgg cgcgctttgc cccctcaaat gccaggcccc
    21121 ttgtttctta tggggttatg gccaatcgcc cccgatcgcg aaaggccccg ctattcccgc
    21181 gcggtcccgc accagcgccg cgcatatcaa aatgacaagc gtcgcggcga ttatcagagc
    21241 cgagaggtag ctgcggtctg gggcatgaaa acgccttcct cgatgaagca catttttgga
    21301 aagacgaaag cgcaagaagt cgttgcacga ctttcgcgaa aggaatgctc ggattcctga
    21361 cacggcaggc gccttccact tgaatgcccc tcaaagggag gaaatcttga gtcaggcgcc
    21421 ggccaaagcc gcaaccaggc cgcccgccgc cagcacccag agcgggttga ggcgggggcg
    21481 acacaggagc accagcgtcg atgcggcggc gatggcccag aggaaagggt cgccgccgcc
    21541 ggcgcggagg atgagcagag agccgccgag gatgaggccg gcggcgaccg gcgccaggcc
    21601 ttgctcgagg gcgctgtgcc agggctggcc gcgagggtcg ctccagcgct tggcgatggc
    21661 gtaatagagg atcgaggagg gcacgaagag agcgagcgat gccactgccg cgcccggcag
    21721 tcccgcgacc ttccagccga ccagcgtcgc cagcagcacg cccgggtcgg gcgcggcgcg
    21781 ggcgatggcg aagagatcga cgaagtcgcg ctcgctcatc caatgccgcg ccgcggtgcc
    21841 cgccccgctt cagaacggtt cgatagggca attcccactt gttcccaaat cgggaacatg
    21901 ctacattgtg atttgtgaat gtcattgctc gccgcacctt ggtggccttc tggagagcgc
    21961 acccgcaccc gcacccgcac ccgcacccgc acccgcaccc gcacccgcac ccgcacccgc
    22021 acccgcaccc ggagacgaga cgccccttgg agcagtggtt gaaa
//
LOCUS       DASYNS010000330         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2576814, whole genome shotgun sequence.
ACCESSION   DASYNS010000330 DASYNS010000000
VERSION     DASYNS010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2576814"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VGP50_10270"
     CDS             <1..373
                     /locus_tag="VGP50_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV8027808.1"
                     /translation="FRLPSIRIEQIRTGRHALVNLTAVTPLGPGESEINHAIYWTLPW
                     LGPLKPLLRPFVRAFLRQDRDVMVLQQEGLRHDPPLLLINDADTQAKWYYRLKNEYAR
                     ARAENRPFVNPVKERVLRWRS"
     gene            509..1471
                     /locus_tag="VGP50_10275"
     CDS             509..1471
                     /locus_tag="VGP50_10275"
                     /inference="COORDINATES: protein motif:HMM:NF021640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061810 - NAD glycohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEV8027809.1"
                     /translation="MNIASAPSASLPDHSRLQLGIAKLRHRIAELEVFHPDQIENRND
                     PAIARLTKGIEDTLVEVFGADTDDYRQYSNAKALDTAPYMLGGTPIGLVRHGLEQGKA
                     TAISLLQQAAASLEQRIGPSQAAPPPIAPKPVPPEHPAVVAPPPPVAAPPPVARPAAP
                     PPLPAAAPAPATVAGTAMSLLVAYAAGDAAGEEIARFLAKTELGAVSRYESAVAGPTD
                     IEAMERHAGIGFAIVVMPPEDTPGASILRQPSGRQKALAELFYFIGKLGRAKVCVLMK
                     GEGEASPVTGMTSASYDRYEGWQKVVLRGLEAAGYRIDWGKALR"
     gene            complement(1438..1614)
                     /locus_tag="VGP50_10280"
     CDS             complement(1438..1614)
                     /locus_tag="VGP50_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027810.1"
                     /translation="MLLLAASCFIAGIAVAFDAVATALQNQRFSAIRVRSLSARHGLP
                     ERPSQRSALPQSMR"
     gene            complement(1855..>2073)
                     /locus_tag="VGP50_10285"
     CDS             complement(1855..>2073)
                     /locus_tag="VGP50_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027811.1"
                     /translation="AVQQHFSGQPTTIDTRYWPQLAVTVQGVDKSSCIEAAQIAGRMD
                     GLVVVQLEKYRSAAECGESNDMTWWILP"
BASE COUNT          341 a          713 c          676 g          343 t
ORIGIN      
        1 ctttcgcttg ccgagcatcc gcatcgagca gatccgcacc ggccgccatg cgctcgtcaa
       61 ccttaccgcg gtgacgccgc tgggtccggg cgagagcgag atcaaccacg cgatctattg
      121 gacgctgccg tggctcggcc cgttgaagcc gctgctccgg ccctttgtgc gcgcgttcct
      181 gcgccaggac cgcgacgtca tggtgctgca gcaggaaggc ttgcgccacg atccgccgct
      241 gcttctcatc aacgacgccg acacccaggc gaagtggtat tatcggctca agaacgaata
      301 cgcccgtgcc cgcgccgaga accgtccctt cgtgaacccg gtgaaggagc gcgtgctgcg
      361 ctggcggagc tagcggattt cgtcgcgaaa tttccgattt ccggcggcta tttccgatta
      421 atttaccaat tccttgacaa agtattatgg aatcgcgcgc catgcggcgg tcttcggatg
      481 atgtcggacg attttggtga cggttctcat gaacatcgcg tctgcgccct cagcttctct
      541 gccggatcac tcccgcctcc agctcggcat cgccaagctg cgtcatcgca tcgccgagct
      601 cgaggtcttc catcccgacc agatcgagaa ccgcaacgat ccggcgatcg cccggctgac
      661 gaaggggatc gaagacacgc tcgtcgaagt gttcggcgcc gacaccgacg actaccggca
      721 atacagcaac gcgaaggcgc tcgacacggc cccctacatg ctcggcggaa cgcccatcgg
      781 cctcgtgcgc cacggcctcg agcagggcaa ggccacggcc atttcgctgc ttcagcaagc
      841 ggcggcatcg ctcgagcaac gaataggtcc gagccaggcc gcaccgccgc ccatcgcgcc
      901 caaacctgtg ccgcccgagc acccggccgt tgtggcgccg cctccgcccg tcgccgcgcc
      961 gccgcctgtc gcccggccgg cggccccgcc gccgctgcct gccgccgcgc ccgctccggc
     1021 gactgtcgcg gggacggcga tgtcgctgct ggtggcgtat gccgcgggcg atgcggccgg
     1081 cgaggagatc gcgcggttcc tggccaagac cgagctcggc gctgtcagcc gctatgagtc
     1141 ggccgtcgcg gggccgaccg acatcgaggc gatggagcgt catgccggga tcggcttcgc
     1201 cattgtcgtg atgccgcccg aggacacccc gggcgccagc atcctgcgcc agccaagcgg
     1261 acggcagaag gcgctggccg agctcttcta cttcatcggc aagctgggtc gcgccaaggt
     1321 ctgcgtcctg atgaagggcg aaggcgaggc ctcgcccgtc accggcatga cctccgcatc
     1381 ttacgatcgc tacgaaggct ggcagaaggt cgtgctgcgc ggcctcgaag cggccggcta
     1441 tcgcatcgac tggggcaagg cgctgcgctg agacggccgc tccggcaggc cgtgacgcgc
     1501 cgacagcgag cggacgcgta tcgccgagaa gcgctgattc tggagcgcgg tcgccaccgc
     1561 gtcgaaggcc acggcaatgc cagcgatgaa gcagctggcg gcgagaagca acatgatgaa
     1621 acctcctggc gggttaaaat tcggccgccc gtccgtgggg gcgtcgggcg ggcggccgaa
     1681 ggagaagacg gtcggatcga gacgaattct cggcggagaa tgacatcccg ggccgccccg
     1741 tcgacccctt gtcctcagac ctaagcagcg ttgctttggc cgcgccacac atccccctct
     1801 ccccggaggg gagaggggat ttgtgcatac gtgtcgcgat ctgtgcaact ctgcttaggg
     1861 caggatccac caggtcatgt cgttgctctc gccgcactcg gcggccgagc gatatttctc
     1921 gagctgcacg acgaccaggc cgtccatgcg gccggcgatc tgcgccgcct cgatgcagct
     1981 gctcttgtcg acgccctgga ccgttacggc gagctgcggc cagtagcgcg tgtcgatcgt
     2041 cgtgggctgg ccgctgaagt gctgctgcac ggc
//
LOCUS       DASYNS010000331         9384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3826029, whole genome shotgun sequence.
ACCESSION   DASYNS010000331 DASYNS010000000
VERSION     DASYNS010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3826029"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..156)
                     /locus_tag="VGP50_10290"
     tRNA            complement(80..156)
                     /locus_tag="VGP50_10290"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(120..122),aa:Met,seq:cat)
     gene            complement(196..1326)
                     /gene="mnmA"
                     /locus_tag="VGP50_10295"
     CDS             complement(196..1326)
                     /gene="mnmA"
                     /locus_tag="VGP50_10295"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV8027812.1"
                     /translation="MNSLGLPKPVRECRVVVAMSGGVDSAVTAALLVEEGFDVVGITL
                     QLYDQGAALGRKGACCAGQDIHDAARVAELLGIPHYVLDYERRFRDAVIEDFADSYVR
                     GETPVPCVRCNQTVKFRDLLAVARDLGADALATGHYARRVAGEHGPELHRAVDESRDQ
                     SYFLFATTLAQLELLRFPLGGRAKTETRALAERFALPVAAKPDSQDICFVPSGHYAAV
                     VSKLRPGAAAPGDVVDEAGRVLGTHPGVIHFTVGQRRGLGVAAAEPLYVLRLEPEARR
                     VVVGPRAALATRRVAVAGINWLGPTPPRDGLPVGVKLRSAQPPVKARFHLDDGSGGEA
                     LLDEPAFGVAAGQACVVYDGTRVLGGGWIRRTDAKAAPAAIA"
     gene            complement(1337..1669)
                     /locus_tag="VGP50_10300"
     CDS             complement(1337..1669)
                     /locus_tag="VGP50_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family 2Fe-2S iron-sulfur cluster
                     binding protein"
                     /protein_id="HEV8027813.1"
                     /translation="MAKMTFIERDGKRREVDAPLGLSVLEIAHKHGVDIEGACEGSLA
                     CSTCHVIVDPEWFELLKDASEDEEDMLDLAFGLTQTSRLGCQIIMTEELDGLTVKLPQ
                     ATRNMMNG"
     gene            complement(1685..2167)
                     /locus_tag="VGP50_10305"
     CDS             complement(1685..2167)
                     /locus_tag="VGP50_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV8027814.1"
                     /translation="MKLFQIEEPDGAPLTGDGPGAAVGIALSPAGAAVAIALGGNAEL
                     LPGADGALRLPMTGVQSRDGAWDRAALGRLLLSLRERAEKALARPVTDAVIALASPDD
                     GARAALTAAAQDAGLGVLRIMEIAAAAALAGGAAADAAALGAAVQAEDDAAARAARAT
                     "
     gene            complement(2198..2536)
                     /locus_tag="VGP50_10310"
     CDS             complement(2198..2536)
                     /locus_tag="VGP50_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEV8027815.1"
                     /translation="MALRQAMTVTDAAAERIQAMLARRGKPSVGIRVGVRSRGCSGLT
                     YTLEYADEKGKFDEIVQYKGVTILIDPKATMFIIGTEMDYVEDKLQSGFTFRNPNEKG
                     RCGCGESFHV"
     gene            complement(2590..3801)
                     /locus_tag="VGP50_10315"
     CDS             complement(2590..3801)
                     /locus_tag="VGP50_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV8027816.1"
                     /translation="MNVSLARFQPANAAPVYLDYQATTPLDRRVLDAMLPFFTEKFGN
                     PHSVTHAYGREAEAAVEEARAEVAALIGAEPREIIFTSGATEANNLAIKGAARFHRDF
                     ARDHVVTVVTEHKCVLESAAALEREGFRVTRLPVDAQGLVDLAALAAVIDERTSVVSV
                     MAANNEIGVLQPLAEIGALCRARGVLFHSDGAQAVGKVPVDVEAMGLDLLSISGHKVY
                     GPKGIGALYVRRRPRARLAPLFDGGGQERGLRSGTLPTPLCVGLGVAARIAGAEMAPE
                     AERLLGLRRRFLGGIRARLPEVTLNGDAERRLPGNLNLSFPGAPALEVMAACPSLALS
                     TGSACTAAAVEPSYVLRALGVSEALAGASLRIGFGRYTIDSEVDFAVDALAAAVRRVA
                     SRMTEAAGAPT"
     gene            complement(3798..4904)
                     /locus_tag="VGP50_10320"
     CDS             complement(3798..4904)
                     /locus_tag="VGP50_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEV8027817.1"
                     /translation="MRLAYLDWNATAPLRPEAAAAMTESLALCGNPSSVHRFGREARR
                     ALEAARAQVAALIGAAAAAIVFTSGGTEANQLALRGVAGRRLLIAAIEHDSVRDAAPA
                     TVVPVTPQGVVDLDALERMLAADPRPALVSLMLANNETGVIQPVAEAARLAHAHGALL
                     HCDAVQAAGKLPLDVGRLGADLLTLSAHKLGGPQGVGALVVVADVPLRALQTGGGQER
                     GRRAGTENLPGIVGFGVACAIAARELDGYAAVATLRDAAQRRLTAMVPEARVYGGNTA
                     RLPNTLCLAMPGVPAATQVMALDLAGVMVSAGSACSSGKVRRSHVLDAMGVPAAAAES
                     AIRISLGWSTTPADIDQLAEAWGALYARRRASAA"
     gene            complement(4924..5391)
                     /locus_tag="VGP50_10325"
     CDS             complement(4924..5391)
                     /locus_tag="VGP50_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV8027818.1"
                     /translation="MRLSTKGRYAVMAMVDLAKHSQGDPIALAEIAERQEISLSYLEQ
                     LFAKLRVGGLVKSVRGPGGGYLLGHPADETRISDVILAVDEPIRATRCTPGAPMGCRG
                     SKARCLTHDLWEELGNQIHLYLSSVTLADVVAQRVLGRSGALHCEQRTALAAQ"
     gene            complement(5414..6133)
                     /gene="cysE"
                     /locus_tag="VGP50_10330"
     CDS             complement(5414..6133)
                     /gene="cysE"
                     /locus_tag="VGP50_10330"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV8027819.1"
                     /translation="MILRNLRDEVQTVLRLDPAARSAWEVVLCYPGFHALLFYRAAHW
                     LWERQWLVLGRWVSYLGRMMTGVEIHPGAKIGRRLFIDHGVGVVIGETAEIGDDVTLY
                     QGVTLGGTKLNRGKRHPTLEDGVIVGAGAKVLGGFTVGKGARIGPNAVVRTEVPPGAR
                     VVAPRSTVVTRERRDARTETQEFMPYGTPGDEIPDPVARALNGLVDEVKALRARIAEL
                     EGERAEERSGLARKTASGGGT"
     gene            6363..7025
                     /locus_tag="VGP50_10335"
     CDS             6363..7025
                     /locus_tag="VGP50_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8027820.1"
                     /translation="MPEVIINGPEGRLEGRYHHSKESNAPIALLLHPHPHHGGTMNNK
                     VVYTLYHAFVRRGFSALRFNFRGVGRSQGSFDRGEGELSDAASALDWLQTYNPNASAC
                     WIGGFSFGAWIGMQLLMRRPEIDGFISVAPPANLFDFSFLAPCPASGLIIMGERDQLV
                     PVEAVQKLVNKLMHQRDIQIDHRIIAGADHFFGGQLESLAAVAEEYVATSHRPRAAAA
                     AR"
     gene            complement(7036..7230)
                     /locus_tag="VGP50_10340"
     CDS             complement(7036..7230)
                     /locus_tag="VGP50_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027821.1"
                     /translation="MAGDKVLFVNDLVLISEKEPEGDIRSITYKIEVDTKPARQVMML
                     TPDVAQALLLGLEHVLKGDE"
     gene            complement(7239..8531)
                     /locus_tag="VGP50_10345"
     CDS             complement(7239..8531)
                     /locus_tag="VGP50_10345"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV8027822.1"
                     /translation="MANVAVIGAQWGDEGKGKIVDWLSQRADVVVRFQGGHNAGHTLV
                     IGNVEYRLSLLPSGVVRPDKLSIIGNGVVIDPWALIAEIEAIGKKGVTVTPRNLRIAE
                     NAALILPLHAEIDRARELALGDKKIGTTGRGIGPAYEDRVGRRAIRLCDLADPASLSD
                     KIDRLLLHHNALLRGLGAPEVTRAPVLEALLAVAPKVLPFADTVWRRLDEARRAGKRI
                     LFEGAQGVMLDVDHGTYPYVTSSTTHPGQAAAGSGVGPATVGYVLGITKAYTTRVGSG
                     PFPTELKDEIGAMLGERGKEFGVVTGRKRRCGWFDAVMVRQAVKVAGITGIALTKLDV
                     LDGFREIKVCTAYRWRGELLDHLPASATVQAELEPVYETVEGWSESTRGARSWAQLSA
                     FAIKYIRRIEELIEAPVALFSTSPERDDTVLVRDPFED"
     gene            complement(8578..>9384)
                     /locus_tag="VGP50_10350"
     CDS             complement(8578..>9384)
                     /locus_tag="VGP50_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEV8027823.1"
                     /translation="RVKGSQLHPDRQVGQVGAELIGSDETSADVEVVALAADALAASG
                     VGALSVDLGLPTLVPALCTALGLAPEAAEAVRRALDHKDAAAVAAVGGRAAALLGKLL
                     DSAGAAEKALKVLAALDLPAEAASERTRLTKVVERLGQSVPDLHVTIDAVENRGFEYH
                     TGVSFTFFAKGVRGELGRGGRYRTGDGAGEPATGFTLYTDTVLRAIARDTPSRRLYLP
                     VGIDPTLARRLRRDGWTTVAGLGPVADAEAEARRLGCGHRLEGEKIVALA"
BASE COUNT         1507 a         3366 c         3100 g         1411 t
ORIGIN      
        1 caaaccgcgg gaccaaacag cattcgacag ccgtcacgaa gctcgagggc aagcatcaga
       61 gtctctagag attacaaggt ggtagcgggg gagggacttg aacccccgac cttgggatta
      121 tgattcccct gctctaacca gctgagctac cccgccaccg agagcgtcgc ggtataagag
      181 agcgccgcca aggcgtcaag caatcgccgc cggcgcggcc ttcgcatccg tgcggcggat
      241 ccacccgccg ccgaggacgc gggtgccgtc gtagacgacg caggcctgcc ctgcggcgac
      301 gccgaacgcc ggctcgtcca gcagggcttc gccgccgctt ccgtcgtcga gatggaagcg
      361 tgccttcacc ggcggctgcg ccgagcggag cttgacgccc accggcagcc cgtcgcgggg
      421 cggtgtcggg ccgagccagt tgatcccggc cacggcgaca cggcgcgtcg ccagtgccgc
      481 gcgcgggccg accacgacgc ggcgtgcctc gggctcgagc cggagcacat aaagcggctc
      541 ggccgcggcg accccgaggc cgcggcgctg gccgacggta aagtggatga cgcccggatg
      601 cgtgccgagc acgcgccccg cttcgtcgac cacgtcgccc ggcgccgcgg cgccgggacg
      661 cagcttgctc acgaccgccg catagtggcc gctcggcacg aagcagatgt cctggctgtc
      721 gggctttgcc gccacgggaa gcgcaaagcg ctcggcgagc gcgcgcgttt ccgtcttggc
      781 gcggccgccc agcggaaagc gcagaagctc gagctgcgcc agggtggtgg cgaagaggaa
      841 atagctctgg tcgcggctct catcgactgc acgatggagc tcgggcccgt gttcgcccgc
      901 gacgcggcgg gcgtaatgac ccgtggcgag cgcatcggcg ccgagatcgc gcgccaccgc
      961 caggaggtcg cggaatttca cggtctgatt gcagcgcacg caagggaccg gcgtctcgcc
     1021 gcggacatag gagtcggcga aatcctcgat caccgcgtcg cggaaacggc gctcataatc
     1081 gagcacgtaa tgcgggatgc cgagaagctc cgccacgcgc gccgcatcgt ggatgtcctg
     1141 gccggcgcag cacgcgcctt tgcggccgag cgctgcgccc tgatcataga gctgaagcgt
     1201 gatgccgacg acgtcgaagc cttcttcgac aagcaacgcc gccgtcaccg cgctgtcgac
     1261 gccgccggac atcgccacca cgacgcggca ctcgcgcacc ggcttcggca gcccgagaga
     1321 attcatgaag gcgcgcctag ccgttcatca tgttgcgcgt cgcctgcggc agcttcaccg
     1381 tgaggccgtc gagctcctcg gtcatgatga tctggcagcc gagccgggac gtttgcgtca
     1441 ggccgaaggc gaggtcgagc atgtcctcct cgtcctcgct cgcatccttc agaagctcga
     1501 accattcggg atcgacgatg acatggcagg tcgagcaggc gagcgagccc tcgcaagcgc
     1561 cttcgatgtc gacgccgtgc ttatgggcga tctcgagcac cgagagcccg agcggcgcgt
     1621 ccacttcgcg acgcttgccg tcgcgctcga tgaaagtcat cttcgccatg agccccctac
     1681 cccgtcacgt cgcgcgcgcg gcccgggccg ccgcatcgtc ctcggcctga actgccgcgc
     1741 cgagcgcggc cgcgtccgcc gcggcgcccc ccgccaacgc cgccgccgca gcgatttcca
     1801 tgatgcgcaa cacaccgagc ccggcgtcct gcgcggccgc cgtgagcgct gcgcgggcgc
     1861 catcgtcggg agacgccagc gcaatcaccg catccgtgac cgggcgcgcc agcgctttct
     1921 ccgcgcgctc gcgcagcgag agcagcaatc ggccaagcgc cgcacgatcc caggcgccgt
     1981 cgcgcgactg gacgccggtc atcggcagac gcagcgcgcc atcggcgccc ggcagaagct
     2041 cggcattgcc gcccaacgcg atggcgacgg ccgcaccggc cggcgagagg gcgatgccga
     2101 ccgccgcgcc cggcccgtcg cccgtgagcg gcgcgccgtc cggctcctcg atctggaaca
     2161 acttcatggc ggcaatcggt cggaactcgc ggagcgatca aacgtggaag gactcgccgc
     2221 agccgcagcg gcccttctcg ttgggattgc ggaaggtgaa gccggactgg agcttgtcct
     2281 ccacataatc catctcggtg ccgatgatga acattgtcgc cttgggatcg atgaggatgg
     2341 tgacgccctt atactggacg atctcgtcga acttgccctt ctcgtcggcg tattcgagcg
     2401 tataagtcag gccggagcag ccgcggctgc gcacgccgac gcggatgccg accgacggct
     2461 tgccacgcct ggccagcatc gcctggatgc gctctgccgc cgcgtccgtc accgtcattg
     2521 cctgcctgag cgccatggtc ttgccactcc tcgttcgtcc ttttagccga tccgtcgttt
     2581 acgcgacctt tatgtaggag cgccggccgc ctctgtcatc ctcgaagcca cccggcggac
     2641 cgccgccgcg agcgcgtcta cggcaaaatc gacttctgaa tcaatggtat agcggccaaa
     2701 tccgatgcgc aaggaggccc cggcgagcgc ctcggaaacg ccgagcgcgc ggagcacgta
     2761 agagggctcg accgcggctg ccgtgcaggc cgacccggtc gacagcgcca ggctcgggca
     2821 ggccgccatg acctcgagcg cgggcgctcc gggaaagctc agattgagat tgcccggcag
     2881 ccgcctttcg gcatcgccgt tgagcgtcac ctccggcagg cgcgcgcgga tgccgccgag
     2941 gaaccggcgc cggaggccga ggagccgctc ggcctccggc gccatctccg ctccggcgat
     3001 gcgcgccgcc acgccgaggc cgacgcaaag cggcgtcggc aaggtgccgg agcgcaagcc
     3061 gcgctcctgc ccgccgccgt cgaagagcgg cgcaagccgc gcccgcggtc ggcgccgcac
     3121 ataaagcgcg ccgatgccct tgggtccgta gaccttgtgg cccgagatcg acaagaggtc
     3181 gagccccatc gcctcgacat cgaccggcac cttgccgacg gcctgtgcgc catcgctgtg
     3241 gaacagcacg ccccgcgcgc ggcagagcgc gccgatctcg gcgagcggct gcaggacccc
     3301 gatctcgttg ttggccgcca tcaccgagac gacagacgtg cgctcgtcga tcaccgccgc
     3361 cagcgccgct aagtcgacga gcccctgcgc gtcgaccggc agccgcgtga cgcggaatcc
     3421 ttcgcgctcc aaggcggccg cgctctcgag cacgcatttg tgctcggtca cgaccgtgac
     3481 gacgtgatcc ctagcgaagt cacggtggaa gcgcgccgcg cccttgatcg cgaggttgtt
     3541 ggcctcggtg gcgcccgagg tgaagatgat ctcgcgcggc tcggcaccga tcagcgccgc
     3601 cacttccgcg cgcgcctcct ccaccgccgc ctcggcctcg cggccataag cgtgggtgac
     3661 ggaatgcggg ttgccgaatt tctcggtgaa gaaaggcagc atggcatcga gcacgcgccg
     3721 gtcgagcggc gtcgtcgcct gatagtcgag atagaccggc gccgcattcg ccggctgaaa
     3781 gcgagccaag gagacgttca tgccgcactc gcccggcgcc gcgcatagag cgcgccccag
     3841 gcctccgcca gttggtcgat atcggcgggc gtcgtgctcc agccgaggct gatgcggatc
     3901 gcgctctcgg ccgcggcggc gggcacaccc atggcgtcga gcacatggct ccgtcgcact
     3961 ttgcccgagg agcaggccga tcccgcgctc accatcacgc cggccaggtc gagcgccatc
     4021 acttgcgtcg ccgcgggcac gcccggcatg gcgagacaca gcgtgttcgg cagccgcgcg
     4081 gtgttgccgc catagacacg ggcctcgggc accatcgccg tgagccggcg ctgcgccgca
     4141 tcgcgcaacg tcgccacggc ggcatagcca tcgagctcgc gcgcggcgat ggcgcaagcg
     4201 acgccgaagc cgacgatgcc cggcagattc tcggtgccgg cacgccggcc gcgctcctgg
     4261 ccgccgccgg tctgcagcgc ccgcagcgga acatcggcca cgaccaccag agctccgacg
     4321 ccctgcgggc cgccgagctt gtgcgccgag agcgtgagga gatcggcccc gagccggccg
     4381 acatcgagcg gcagctttcc cgccgcttgc acggcgtcgc aatgaagaag cgcgccatgc
     4441 gcgtgggcga gacgcgccgc ttcggcaacc ggctggatga cgcccgtctc attgttggcg
     4501 agcatgagcg ataccagcgc cgggcgcgga tcggcggcaa gcatgcgctc gagcgcatcg
     4561 agatcgacga cgccctgcgg cgtcaccggg acgacggtcg ccggggctgc gtcgcgaacc
     4621 gaatcatgct cgatcgcggc gatcaagagc cgccgtccgg caacaccgcg caacgccaac
     4681 tggttcgctt ccgtgccgcc gctggtgaag acgatcgccg ccgccgcggc gccgatgagt
     4741 gccgccactt gcgcgcgcgc cgcctcgagg gcgcggcggg cctcgcgccc gaagcgatgc
     4801 accgaggacg gattgccgca gagcgccagt gactccgtca tggccgccgc cgcttccggg
     4861 cgaagcggcg ccgtcgcgtt ccagtcgaga taggcgaggc gcatgggcaa aggccgtggg
     4921 cgactattga gcggcgagcg ccgtgcgctg ctcgcaatga agcgcgccgc tgcggccgag
     4981 cacgcgctgc gccacgacat cggccagcgt caccgagctc agatagagat gaatctgatt
     5041 gcccagctcc tcccagagat catgggtgag gcagcgggcc ttgctgccgc gacagcccat
     5101 cggtgcgccc ggcgtgcagc gcgtcgcgcg gatcggctcg tcgacggcca ggatcacatc
     5161 cgagatgcgt gtctcatcgg cggggtggcc caggagatag ccgccgcctg gtccgcgcac
     5221 gctcttgacg aggccgccga cgcgcagctt cgcaaagagc tgctcgagat aggaaagcga
     5281 aatctcctgg cgctcggcga tctcggcgag cgcgatcggg tctccctggc tgtgtttggc
     5341 gagatcgacc atcgccatca ccgcatagcg acccttggtg ctgagcctca tgatgccgcc
     5401 ccttcccgtg tcttcaagtc ccaccgccag acgccgtctt gcgcgccagc ccgctccgct
     5461 cctccgcgcg ctcgccttcg agctcggcga tccgggcgcg caacgccttc acctcgtcga
     5521 ccagaccgtt gagggcgcgg gcgaccggat cgggaatctc gtctccgggc gtgccataag
     5581 gcatgaactc ctgcgtctcc gtccgcgcgt cccggcgctc gcgcgtcacg acggtcgagc
     5641 gtggcgcgac cacgcgggcc ccgggcggca cttcggtgcg caccacggcg ttgggaccga
     5701 tgcgcgcccc cttgccaacg gtgaagccgc ccaggacctt ggcgccggca cccacgatga
     5761 ccccgtcctc gagggtcgga tgccgcttgc cgcgattgag cttcgtccca ccgagcgtca
     5821 ccccctgata gagggtcacg tcatcgccga tctcggccgt ctcgccgatc accacgccaa
     5881 cgccatgatc gatgaaaagc cgccggccga tcttggcgcc gggatgaatc tcgacgcctg
     5941 tcatcatgcg gccgagatag gaaacccagc gtccgagcac cagccattgc cgctcccaga
     6001 gccaatgcgc cgcgcgatag aagagcagcg catgaaagcc cggatagcac agcacgacct
     6061 cccaggccga ccgcgccgcc ggatcgagcc tgagcacggt ctggacttca tcgcgcaggt
     6121 ttctcaagat catgggagcc gtcatgcccg ctgtggtatg atgagcgcta attgccggcc
     6181 ctgtcgtctt cccgcgcccc gacatcgaac gcgacagacg gcggccgctc cgtgaccgtc
     6241 gtcgatatat cttaccccag tagaatagtc aagaatagcg agctggaaat ggccaattca
     6301 ggcaaggaag tcctagtggg ttactgggac aaaaatcggc agtcccggtc agaggagcgg
     6361 gcatgccgga ggtgatcatc aacgggccgg aaggccggct cgagggtcgt taccaccact
     6421 cgaaggagag caatgcgccg atcgcgctgc tgctgcatcc gcatccgcat cacggcggca
     6481 cgatgaacaa taaggtcgtc tacaccctct accacgcttt cgtgcgccgc ggcttctcgg
     6541 cccttcgctt caattttcgc ggggtcgggc gcagccaggg cagcttcgat cgcggcgagg
     6601 gcgagctttc ggacgcagcc tcggcgctcg attggctgca gacctacaac cccaacgcct
     6661 cggcctgctg gatcggcggc ttctccttcg gcgcgtggat cggcatgcag ctgctgatgc
     6721 gccggccgga gatcgacggc ttcatttccg tcgcgccgcc cgccaatctc ttcgacttca
     6781 gcttcctcgc cccctgcccc gcttccggcc tcatcatcat gggtgagcgc gaccagctcg
     6841 tcccggtcga ggcggtgcag aagctggtga acaagctgat gcatcagcgc gacatccaga
     6901 tcgaccaccg catcatcgcc ggcgccgatc acttcttcgg cggccagctc gaatcgctcg
     6961 cggccgtggc cgaggaatat gtcgcgacca gccaccgtcc gcgcgccgcc gccgcggcgc
     7021 gatgaaggcg cgcgcctact cgtcgccctt caggacgtgc tcgagcccca gcagcaatgc
     7081 ctgcgcgacg tccggcgtca gcatcatgac ttgccgcgcg ggtttggtat cgacctcgat
     7141 cttgtaggtg atcgatcgga tgtcgccttc gggctctttc tcgctgatca gcacgagatc
     7201 gttgacgaag agcaccttgt ccccagccat ggttcctctc agtcttcgaa gggatcgcgc
     7261 accaacacgg tatcgtctcg ctccgggctc gtcgagaaca gcgcgaccgg cgcctcgatc
     7321 aattcctcga tgcggcgaat gtatttgatg gcgaacgccg acagctgcgc ccaggatcgc
     7381 gccccgcgcg tgctttcgct ccacccctcg accgtctcgt agaccggctc gagctccgcc
     7441 tgcacggtgg cgctcgccgg cagatgatcg agtagctcgc cgcgccagcg ataggccgtg
     7501 cacaccttga tctcgcggaa gccgtccagc acgtcgagct tcgtaagggc gatgccggtg
     7561 atgccggcga ccttgacggc ctggcgcacc atcaccgcgt cgaaccagcc gcagcgacgc
     7621 ttccggcccg tgaccacgcc gaactccttg ccgcgctcgc cgagcatcgc gccgatctcg
     7681 tccttgagct cggtcggaaa aggaccgctg ccgacacgcg tcgtataggc cttggtgatg
     7741 cccagcacgt agccgacggt cgcgggtccg acgccggaac ccgcggccgc ctgtcccgga
     7801 tgcgtcgtgg aagaggtcac gtaggggtag gtcccgtgat cgacgtcgag catcacccct
     7861 tgcgcgcctt cgaagaggat tcgcttgccg gcgcggcgcg cctcgtcgag acgccgccag
     7921 accgtgtcgg cgaagggcaa tactttcggc gccaccgcga gcagcgcctc gagcactggc
     7981 gcgcgcgtca cctccggcgc gccaaggccg cgcagcagcg cgttgtgatg gagcaacaat
     8041 cggtcgatct tgtcgctgag cgacgcaggg tcggcgaggt cacagaggcg gatggcgcgg
     8101 cggccgaccc tgtcctcgta agccgggccg atgccgcggc cggtggtgcc gatcttcttg
     8161 tcgccgagcg ccagttcgcg ggcgcggtcg atctcggcgt gcagcggcag aatgagggcg
     8221 gcgttctcgg cgatccgcag attgcgaggc gtgaccgtca cgcctttctt gccgatggcc
     8281 tcgatctcgg cgatgagggc ccagggatcg atgacgacgc cattgccgat gatcgacagc
     8341 ttgtccggcc gcaccacgcc cgacggcagc agactcaggc ggtattcgac attgccgatg
     8401 accagagtgt ggccggcatt gtggccgccc tgaaaacgca ccacgacgtc ggcgcgctgc
     8461 gacagccagt cgacgatctt gcccttgccc tcgtcgcccc attgggcgcc gatcaccgcc
     8521 acattggcca tgtcgcgaac gtctcctgca tctccgggcg cttctgcccg ccgcccctca
     8581 cgcgagcgct acgatcttct caccttcgag acgatggccg caaccgagcc gccgcgcttc
     8641 agcctcggcg tcggcgaccg gcccaagccc ggccaccgtc gtccagccat cgcgccgcaa
     8701 ccgtcgtgcc agcgtgggat cgatgccgac gggaagatag agccgccgcg acggcgtatc
     8761 cctggcgatc gcgcggagca cggtgtcggt atagagcgtg aagccggtcg cgggctcgcc
     8821 ggcgccgtcg ccggtgcgat agcgcccgcc gcggccgagt tcaccgcgaa cgcctttggc
     8881 gaaaaacgtg aagctgaccc cggtgtgata ctcgaagccg cgattctcga cggcatcaat
     8941 cgtgacgtgc aggtccggga ccgattggcc cagccgttcg acgaccttgg tgaggcgcgt
     9001 ccgctcgctg gccgcttccg ccggaaggtc gagcgccgcc aggaccttga gcgccttctc
     9061 cgccgcgccg gcgctgtcga gcagcttgcc cagcagcgcg gcagcgcggc cgccgaccgc
     9121 ggccactgct gcggcatcct tgtgatcgag cgcgcgccga accgcctccg ccgcctccgg
     9181 cgcaaggccg agcgccgtgc acagcgccgg caccagagtc ggcaggccga gatcgacgga
     9241 gagcgcgccg acaccgcttg ccgccagtgc gtcggccgcc agcgccacca cctcgacatc
     9301 ggcgctggtt tcgtccgagc cgatgagctc ggcgccgacc tgtcccacct ggcgatccgg
     9361 atgaagctga ctgcctttga cgcg
//
LOCUS       DASYNS010000332         8983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4606326, whole genome shotgun sequence.
ACCESSION   DASYNS010000332 DASYNS010000000
VERSION     DASYNS010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4606326"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..700)
                     /locus_tag="VGP50_10355"
     CDS             complement(158..700)
                     /locus_tag="VGP50_10355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8027824.1"
                     /translation="MTAPPGAAGIVIRDAADADFAAITDIYAHHVRFSLGSFEEIPPT
                     LEEMARRRGDVLARNLPYLVAVNPSGAVRGYAYAAPYRARTAYRFSVEDSVYVAPDAG
                     RQGIGRRLVAELIERCAKTGCRQMVAVIGDSGNIASISLHEQLGFRHVGILTAIGFKQ
                     GRWVDSVLMQRALGEGADRP"
     gene            complement(697..1587)
                     /locus_tag="VGP50_10360"
     CDS             complement(697..1587)
                     /locus_tag="VGP50_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEV8027825.1"
                     /translation="MEGIFERRAAVEPPIDVLAPAEQLLPLVFASPHSGSDYPSEFLA
                     ASRLDPLTLRRSEDSYVDEIFGAVPTLGAPLLRARFPRAYLDPNREPFELDPAMFEDE
                     LPAFVNTRSPRVRVGLGTIARVVATGEDIYARKLQVSEAMMRIDRLYRPYHRALRQLV
                     TTTRERFGNYLLIDCHSMPSAVMPGERGARRHRTDIVLGDCHGTACHPLVMETAHRLL
                     SGKGYAIARNSPYAGGFTTAHYGKPAANGHCLQIEIGRALYMDERSFERKPFLAQLIE
                     DMRDLAAALADIDLALLAAA"
     gene            1731..2090
                     /locus_tag="VGP50_10365"
     CDS             1731..2090
                     /locus_tag="VGP50_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8027826.1"
                     /translation="MARILLAEDDDNMRIFLARALRRAGHDVHAVPDGDAAFAWVRER
                     PVDLLLADVVMPGLDGIELARRANDLWPDLRVMFITGFAAVALRQRGATKREAAVLAK
                     PFHLRQLVTEIDRFLAA"
     gene            2167..2241
                     /locus_tag="VGP50_10370"
     tRNA            2167..2241
                     /locus_tag="VGP50_10370"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Val,seq:gac)
     gene            complement(2292..4079)
                     /locus_tag="VGP50_10375"
     CDS             complement(2292..4079)
                     /locus_tag="VGP50_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV8027827.1"
                     /translation="MSRPIQDYALISDCRSAALVSRDGSIDWLCWPRFDSGACFAALL
                     GTAENGRWKIAPADAAARVARRYRDGTMILETEFETAAGAATLIDFMPPGGDTSAVVR
                     LVVGRRGRVDFFTEMVIRFDYGISVPWVSRLPDDALSAIAGPNFVVLRTPVPLRGADL
                     RTVGEFSVAAGDTLPFVLAHGASHRSPPQPIDAGAALAATEAFWREWSGRCPSLGVWT
                     EPVKRSLMVLKSLTYAPTGGIVAAVTTSLPEQIGGPRNWDYRYCWLRDASLVLLAFMK
                     LGYYDEARDWRDWLLRSLAGSPEQMRIMYGIAGERYLPEWELPWLPGYENSRPVRVGN
                     AAASQIQIDVYGELADAMFTAVTGGFPPAPRQLAFRDVVLPHVERVWKEPDSGIWEVR
                     GPLRHFTHSKVMAWVAFDRSARLLDMSGDSRAAAHWRGVAQQIHDDVCAKGFDPQLGS
                     FVQSYGSKLLDASLLQLPLVGFLPPSDPRIRGTIVAIEQRLRDESGLVRRYDTETGID
                     GLPPGEGAFLACSFWLADNYALQGRLAEARALFENLLAKQNDVGLLAEQCDPRTGRML
                     GNFPQAFSHVGLITTALNLVRAEAPREER"
     gene            4518..5708
                     /locus_tag="VGP50_10380"
     CDS             4518..5708
                     /locus_tag="VGP50_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027828.1"
                     /translation="MGKTRLTKIGGMVAMLALSLVIAGDEARAGDTVKIGLILTYSGS
                     FADNARQIDNGVQLYMKQHGDSVAGKKIEIIRRDSTGAAPDVAKRLAQELVTRDKVDF
                     LAGFVLTPNALAVADVATEAKVPMVIMNAATSIITTKSPYVARVSFTLPQVTVPLAQW
                     AAKSGIKQVYLMTSDYGPGHDAEAAFEKAFTAAGGSIVGTVRMPLASPDFAAYVQKVN
                     DLHPQAVFLFVPAGQQPAALMRTFAEQGLTRSSLQILATGDVTDDAVLQRMGDAALGI
                     ITTHHYSYAHDSALNHAFVKAYAEAYGEDLRPNFMAVGGYDGMAAICRVIAELKGEMN
                     ADKAMAVLKGMAIESPRGAIEIDPETRDVVQTVYVRRVEKVGDTLVNVEFDQVAKVKD
                     PGKP"
     gene            complement(5717..6106)
                     /locus_tag="VGP50_10385"
     CDS             complement(5717..6106)
                     /locus_tag="VGP50_10385"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEV8027829.1"
                     /translation="MSMEIVKEFRFDAAHFLPTAPEGHPYRRLHGHSFRVAVTIAGSP
                     DPVTGWLADFAEIERALLRLRDGLDHRLLNEVAGLAVPTLENLAFWIHGALAGALPGL
                     AAVTIHRESAGESCTYRPILDSNPSTE"
     gene            complement(6103..6738)
                     /gene="queE"
                     /locus_tag="VGP50_10390"
     CDS             complement(6103..6738)
                     /gene="queE"
                     /locus_tag="VGP50_10390"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEV8027830.1"
                     /translation="MTYVVKEIFYTLQGEGANSGRPAVFCRFAGCNLWSGREADRTDA
                     VCRFCDTDFVGTDGPGGGRFAGARELAAGVRRRWPAESGARPFVVCTGGEPLLQLDAA
                     LIDALHAEGCAVAVETNGSCPAPDGLDWICVSPKASAPLALARGDELKIVFPQEGLDP
                     RAFEHLDFRHFFLQPMDGPEREGNTALAVRFCLAHPRWRLSLQTHKLIGIP"
     gene            complement(6735..7475)
                     /gene="queC"
                     /locus_tag="VGP50_10395"
     CDS             complement(6735..7475)
                     /gene="queC"
                     /locus_tag="VGP50_10395"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018980502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEV8027831.1"
                     /translation="MAAQTIERPGGAAAEKAIVLFSGGQDSTTCLAWALERFAEVETV
                     GFDYGQRNSIELACRETVLAEMRQGFAPWALRLGDDHKVDLSALGRLSETALTREVAI
                     VLGENGLPTTFVPGRNLLFLTFAAAIAYRRGARHLVAGVCETDFSGYPDCRDDTIKAM
                     QVALNLGMERRFVIDTPLMWIDKPATWALAERLGGMALVDLIREHTHSCYLGERGRRW
                     AWGYGCGACPACTLRASGYERYRDAPRR"
     gene            7772..8626
                     /locus_tag="VGP50_10400"
     CDS             7772..8626
                     /locus_tag="VGP50_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027832.1"
                     /translation="MGGGMLIDPRGRVWPDDSWDVARRVGYNDVRDDVATFAVRERGF
                     VHLRPRTDGMHVALREGRFNLTAFAGLMLELGRTRPPRVLLRVLADDGPKFQLFTELH
                     DLCAHLEALSRDKPVQIRIPRLAERRNLRVLTLPTFAPARPIVDLWERTRGELTEEVH
                     GALQSGDLLPRTILVRQRAEGSRLVIQHFGTGIMIMQPDEALLAVGRDLEDQPDREYG
                     EWMAKAYADTLLRRCVRVDSCRALVRTSAATTLRTRYDRVLMPWRSGNDLLVMCLSLQ
                     REMPIAVG"
     gene            complement(8547..>8983)
                     /locus_tag="VGP50_10405"
     CDS             complement(8547..>8983)
                     /locus_tag="VGP50_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027833.1"
                     /translation="VAAGDRVEVDASSAEHFYGRIMFSGAVWVRPDYRRHGVTKIVPR
                     LTRSYALARWNTPVFWAYIDHDLDKIGVTRAYGSWHVEDGIMTHMPSWRGDHRILFLS
                     MGQTSLIRDIAGSIARASQRRSASPSARRGTSRADHFRSATA"
BASE COUNT         1571 a         2908 c         3053 g         1451 t
ORIGIN      
        1 gagggtcata ttgtgcagtg cggcattggt cgtcgcccga cggcctgccg tacgtcttct
       61 aggcgtttcc tccctaaact tgggccgtgt gcaacatcac ggcccatttt ttttgcgctg
      121 caaacggcgc cgcgccgggc cgccgaggcc aaccgcttca ggggcggtca gcaccctcgc
      181 cgagcgcacg ctgcatcagc acagaatcta cccaacggcc ctgcttgaag ccgatcgcgg
      241 tgagaattcc gacatggcgg aagcccagct gctcatggag ggagatcgag gcgatgttgc
      301 cgctgtcgcc gatcaccgcc accatctggc ggcagcccgt cttggcgcag cgctcgatca
      361 gctcggcgac gagcctgcgg ccgatcccct ggcgtccggc gtcgggcgcc acatagacgg
      421 agtcctcgac gctgaagcga taggcggtgc gggcgcgata gggggccgca taggcatagc
      481 cgcggaccgc cccggagggg ttgacggcga ccagataggg aaggttgcgg gcgagcacgt
      541 cgccgcgccg ccgggccatc tcctcgagag tggggggtat ttcctcgaaa gagccgaggc
      601 tgaaacggac gtgatgggca taaatgtcgg taatggccgc gaaatccgcg tcggcggcgt
      661 cgcgaatgac gatgccggcc gcgcccggcg gcgcggtcat gccgcggcga gcagcgccag
      721 gtcgatatcc gccagcgccg cagcgagatc gcgcatatcc tcgatcagct gggcgaggaa
      781 gggcttccgc tcgaagctgc gctcatccat gtagagcgcc cgcccgatct cgatctgcag
      841 gcaatggccg ttggcggccg gcttgccata atgggcggtg gtgaagccgc cggcgtaagg
      901 gctgttgcgc gcgatggcgt agcccttgcc gctcaagagg cgatgcgccg tctccatgac
      961 gagcggatgg cacgcggtgc cgtggcaatc gccgagcacg atatcggtgc gatgccggcg
     1021 cgcaccgcgc tcgccgggca tgacggcgga gggcatcgag tggcagtcga tcagcagata
     1081 gttgccgaag cgctcgcgcg tcgtcgtcac gagctggcgc agcgcgcgat gataggggcg
     1141 atagagccga tcgatccgca tcatcgcttc gctgacctga agcttccgcg cgtagatgtc
     1201 ctcgcccgtg gctaccaccc gtgcgatggt cccgagcccg acgcgcacgc gcggggagcg
     1261 cgtgttgacg aaggccggga gctcgtcctc gaacatcgcg ggatcgagct cgaagggctc
     1321 gcggttggga tcgagatagg cccggggaaa ccgcgcgcgc aggagcggcg cgccgagtgt
     1381 cggaaccgcg ccgaagatct cgtcgacata gctgtcttcc gagcgtctta gggttaacgg
     1441 gtcgagccgc gacgccgcca ggaactcgct cgggtaatcg ctgccgctgt ggggcgaagc
     1501 gaagacgagc ggcagcagct gctccgccgg cgccagcacg tcgatcggcg gttcgacggc
     1561 cgctcttcgc tcgaatatcc cttccatcgg tccgagatta gggggccgcg gcttctctgt
     1621 cacctgctaa gcagtacatg ccgaaatctt cttggtgaac gcgcgtgatc ttgtcgcgcg
     1681 ccgcgcgcag gacgagtaga gtgcgccctg ggtaggggcc cggagcgttt atggcgcgta
     1741 ttctccttgc cgaggacgac gacaacatgc ggatttttct ggcccgcgcg ttgcgccgtg
     1801 ccggtcatga tgtgcatgcg gtcccggacg gcgacgcggc cttcgcctgg gtgcgcgagc
     1861 gacccgtcga cctcttgctg gccgacgtcg tcatgcccgg cctcgacggc atcgagctgg
     1921 cccggcgcgc caacgatctc tggcccgacc tgcgcgtgat gttcatcacc ggcttcgccg
     1981 cggtggcgct gcgccagcgc ggcgccacga agcgcgaggc ggccgtgctc gcgaagcctt
     2041 tccatctgcg tcagctcgtg accgagatcg accgctttct cgcggcgtga ggctgcggcg
     2101 cgcagagttc gcggcgcttc ttgccaggac cggcgatttg gtttacatcg ctcgtcgcga
     2161 gcgcgtgggc acgtagctca gcgggagagc accgccttga catggcgggg gtcacaggtt
     2221 caatccctgt cgtgcccacc acgcatcaac gcccgcgcat cccgttagcg ccgaccatcg
     2281 cccatcacat tctatcgctc ttcgcgcggg gcttcggccc ggacgagatt gagggcggtg
     2341 gtgatgaggc cgacatggct gaacgcctgc ggaaaattgc cgagcatccg gccggtgcgg
     2401 ggatcgcatt gctcggcgag aagcccgacg tcgttttgct tggcgagcaa gttttcgaag
     2461 agcgcccggg cctcggcgag gcggccctgg agcgcgtagt tgtcggcaag ccagaagctg
     2521 caagcgagaa acgcgccttc gcccggcggc agcccatcga tgccggtttc ggtgtcatag
     2581 cgccgcacca gcccgctctc atcgcgcaga cgctgctcga tcgccacgat cgtcccgcga
     2641 atccggggat cgctcggcgg caggaagccg accaggggga gctggagcag gctcgcatcg
     2701 agaagcttcg atccatagga ctgaacgaag ctgccgagct gaggatcgaa gcccttggcg
     2761 cagacgtcgt catgaatctg ttgcgcgacg ccgcgccaat gcgcagcggc gcgactgtcg
     2821 ccgctcatgt cgagcagcct ggcggaccgg tcgaacgcca cccaggccat gaccttcgaa
     2881 tgcgtgaaat gccgcagcgg accgcgcact tcccagatgc cgctatcggg ctctttccag
     2941 acccgctcga catgaggaag cacgacatca cgaaaggcca gctgccgcgg ggccggcggg
     3001 aaccccccgg tgaccgccgt gaacatggca tcggcgagtt cgccataaac gtcgatctgg
     3061 atttggctcg ccgcggcgtt gccgacgcgg acaggacgcg agttctcata gcccgggagc
     3121 cagggcagtt cccattccgg caagtaccgc tctccggcga ttccatacat gatgcgcatc
     3181 tgctccggac tgccggcgag cgagcgcagc agccagtcgc gccaatcgcg cgcttcgtcg
     3241 tagtaaccga gcttcatgaa ggcgagcagc accaagctcg catcgcgcag ccagcagtag
     3301 cgatagtccc aattgcgcgg cccaccgatc tgctcgggaa gcgaggtcgt caccgccgcg
     3361 acgatgccgc cggtcggcgc gtaggtcagc gacttcagga ccatgagcga ccgcttcacc
     3421 ggctcggtcc agacgcccag ggacgggcag cggcccgacc attcgcgcca gaacgcctcg
     3481 gtcgccgcca atgccgcgcc ggcgtcgatc ggctggggcg gggagcgatg ggaagcgcca
     3541 tgcgcgagca cgaagggcag cgtgtctccg gcggcgacgg agaattcgcc gaccgtgcgt
     3601 aaatcggcgc cgcgcaaagg cacgggcgtg cgcagaacca cgaaattggg tccggcgatg
     3661 gccgagagcg cgtcgtcggg caggcggctc acccagggga cgctgatgcc gtaatcgaag
     3721 cggatgacca tctcggtgaa gaagtcgacc cggccgcgcc gcccgaccac gagacggacc
     3781 acggcggagg tgtcgccgcc gggcggcatg aagtcgatga gggtggccgc gccggcagcc
     3841 gtctcgaact cggtctcgag aatcatcgtg ccgtcgcgat agcggcgcgc gacccgcgcc
     3901 gccgcatcgg cgggcgcgat cttccagcgg ccattctcgg cggtgccgag cagcgccgcg
     3961 aagcatgctc cggaatcgaa gcgcggccag cacagccaat cgatcgaacc gtcgcggctc
     4021 acgagagcgg cggatcggca gtcgctgatg agcgcatagt cctggatcgg tctcgacatg
     4081 tacgaccttt cgcggtggtc atgcccggca gagccggacg ctccacgatc gacaaagaac
     4141 gtggggccac cgacatcctc cggggcgccg aaaacagccg gaacgcaaac gccgcttcat
     4201 gacgagcgtt gcgatccgat gaaagggcct atttggaaca agtccaaaga tacacggcac
     4261 gtctggagtc ctgtgtcata actgcggcct ccgccctgcc gcgtgccgcg tcggggaatt
     4321 gaagacggca accgggtgtt cctctaaggg tggccccggc gcggccacct cctccccgga
     4381 cttgggccgc gtatcgctgc gcggccaatt tttcaccgcc cggcgcggcg cctgcgtcgc
     4441 ttgtcgcggg cgagccggcg gcttattgct gggcggacaa gaaaggccgc ggtgcgcgtg
     4501 ccaatcggga gggggctatg ggaaaaacgc gtttgacgaa gatcggcggc atggtcgcca
     4561 tgctggcctt gagtttggtg atcgcgggcg acgaagcacg cgcaggcgac accgtgaaga
     4621 tcggcttgat cctcacctat tcgggatcct tcgccgacaa cgcgcggcag atcgacaacg
     4681 gcgtccagct ctatatgaag cagcatggcg atagcgtcgc cggaaagaag atcgagatca
     4741 tccgtcgcga tagcaccggg gccgcgcccg atgtggccaa gcgcctggcg caagaactgg
     4801 ttacgcgcga caaggtcgat ttcctcgcgg gcttcgtgct gacgcccaac gcgctcgccg
     4861 tcgccgacgt cgcgaccgag gcgaaggtgc cgatggtgat catgaatgcc gcaacctcga
     4921 tcatcaccac caagtcgccc tacgtcgcac gcgtctcctt caccttgccg caggtgaccg
     4981 tcccactcgc gcaatgggcg gccaagagcg gaatcaagca ggtctatctg atgacctcgg
     5041 actacgggcc gggtcacgac gcggaggcgg cgttcgagaa agccttcacc gcagccggcg
     5101 gatcgattgt cgggacggtg cgcatgccgc tcgccagccc cgatttcgcc gcctatgtgc
     5161 aaaaggtcaa cgaccttcat ccgcaagcgg tctttctctt cgtgccggcg ggacagcagc
     5221 cggcggcgct gatgaggact ttcgccgagc agggactgac gcggtcgagc ctgcagatcc
     5281 tcgccaccgg cgacgtcacc gacgatgccg tgctccagag aatgggtgat gccgccctcg
     5341 gcatcatcac cacgcatcac tactcctatg cgcacgactc cgcgctcaac cacgccttcg
     5401 tcaaagctta tgccgaggcc tacggcgagg atctccggcc gaatttcatg gcggtcggcg
     5461 gctatgacgg gatggcggcg atctgtcgcg tgatcgccga gctcaagggc gagatgaacg
     5521 ccgacaaggc gatggcggtg ctcaagggca tggcgatcga gagcccgcgc ggtgccatcg
     5581 agatcgatcc cgagacccgc gatgtcgtcc agaccgtcta tgtgcggcgc gtcgagaaag
     5641 tcggcgacac gctcgtcaat gtcgagttcg accaggtggc gaaggtgaaa gatccgggca
     5701 agccgtagac ggccgcttac tctgtcgacg gattactgtc gagtatcggc cggtatgtgc
     5761 agctttcgcc ggccgactcg cggtggatgg tgacggccgc gagaccgggc agcgcgccgg
     5821 ccagcgcccc atggatccag aaagcgaggt tctcgagggt cggcaccgcg agacccgcga
     5881 cctcgttgag cagacgatgg tcgaggccgt cgcgcagccg cagcagcgcg cgctcgattt
     5941 cggcgaaatc cgcgagccag cccgtgaccg gatcgggcga accggcgatc gtcacggcga
     6001 cgcggaagga atggccgtga aggcggcgat agggatggcc ttcgggcgcg gtggggagga
     6061 aatgcgcggc gtcgaagcgg aattccttga cgatctccat actcacggga tgccgatcag
     6121 cttgtgggtt tggaggctga gccgccagcg gggatgggcg agacagaagc gcacggccaa
     6181 ggcggtgttg ccttcccgct ccgggccgtc catcggctga aggaagaagt ggcggaaatc
     6241 gaggtgctcg aacgcccggg gatcgagccc ttcttgcgga aagacgatct tcaactcgtc
     6301 gccccgagcg agcgccagcg gtgcactcgc cttcgggctg acgcagatcc agtcaaggcc
     6361 gtccggcgca gggcagctac cgttcgtctc gacggcgact gcgcagcctt cggcatgaag
     6421 cgcgtcgatc agcgccgcat cgagctggag caagggctcg cctcccgtgc agacgacgaa
     6481 cggacgcgct ccactctctg cgggccagcg gcggcgcacg cctgccgcga gctcgcgggc
     6541 accggcgaag cggccgcccc cggggccatc ggtgccgacg aagtcggtgt cgcagaagcg
     6601 gcagaccgcg tccgttcgat cggcctcgcg gccgctccag aggttgcagc cggcaaagcg
     6661 gcagaagacg gcagggcggc cgctattcgc tccctcgccc tggagcgtgt agaagatctc
     6721 cttcacgacg taggtcatcg gcgaggcgcg tcgcgatagc gctcataccc gctagcgcgc
     6781 agcgtgcaag ccgggcacgc gccgcagcca tagccccacg cccatcgccg cccgcgctcg
     6841 cccagatagc agctatgggt gtgctcgcgg atgaggtcga cgagcgccat gccgcccagc
     6901 cgctcggcca gcgcccaggt cgccggcttg tcgatccaca tcagcggcgt gtcgatgacg
     6961 aagcggcgct ccatgccgag attgagcgcc acctgcatgg ccttgatggt gtcgtcgcgg
     7021 cagtcgggat agcccgagaa atcggtctcg cacacgcctg cgaccagatg ccgggcaccg
     7081 cggcgatacg cgatcgccgc agcgaaggtg aggaacagca ggttcctccc cggcacgaaa
     7141 gtcgtgggca agccgttctc gcccaggacg atggcgacct cgcgggtgag cgcggtctcg
     7201 ctgagcctgc cgagcgcgct cagatcgact ttgtgatcgt cgccgagccg gagagcccaa
     7261 ggcgcgaaac cttgtcgcat ctcggcgagc acggtctcgc ggcaagcaag ctctatgctg
     7321 ttgcgctggc cgtagtcgaa cccgacggtc tcgacctcag cgaagcgttc gagagcccaa
     7381 gcgagacagg tggtcgagtc ctgaccaccg gaaaagagaa cgatcgcctt ttccgccgcg
     7441 gcgccgcccg gccgttctat cgtctgtgct gccaccgggc tttttaggac gccccggcgt
     7501 cgcggctcaa ggtccgacct cgggcggccg cacgatctgg atcaatgcag cgcggtagcg
     7561 cgcacccaga tgcgcaaccc taggatgtat tagaaaagga gttcctcgcg aagtacgcag
     7621 caagcaatgg atggaattcg cagcgcgcgc ccgcatctat gaagagtaat tcaatttgcg
     7681 ggatgattct agaaaaaatg gtttatagta ctcacgctga gttaacggct ccggccggaa
     7741 acgacgtctg gccgggagcc tggaggcggg catgggcggc ggcatgttga tcgatcccag
     7801 aggccgagtc tggccggatg attcctggga tgtcgcgcgc cgcgtcggct acaacgacgt
     7861 ccgcgacgac gtcgccactt tcgccgtccg ggaacgcggc ttcgtccatc tccgcccgcg
     7921 caccgacggg atgcatgtcg cgttgcgcga gggccgcttc aatctcacgg cgttcgcggg
     7981 tctcatgctc gagctcggcc gtacgcggcc gccgcgcgtt ttgcttcgcg tgctggccga
     8041 tgacgggccc aagttccagc tcttcaccga gctgcacgac ctctgcgccc atctcgaggc
     8101 tctttcccgg gacaagcccg tccagatccg cattccgcgc ctcgctgagc ggcgcaacct
     8161 tcgcgtcctg acgctgccga ccttcgcgcc ggcgcggccg atcgttgatc tctgggagcg
     8221 gacgcgcggc gagttgacgg aggaggtcca tggcgccttg cagtccggtg acttgctccc
     8281 gcgcacgatc ctggtgcggc agcgggcaga gggttcgcgt ctcgtgatcc aacacttcgg
     8341 caccggcatc atgatcatgc agccggacga ggctctgctc gctgtcggcc gcgatctcga
     8401 ggatcagccg gatagagaat atggcgagtg gatggcgaaa gcctacgcgg acaccctgtt
     8461 gcgtcggtgc gttcgcgtgg attcctgccg agcgttggtt cgcacctccg cggcgacgac
     8521 gttgcgcacg cgctatgaca gggtcctcat gccgtggcgc tccggaaatg atctgctcgt
     8581 gatgtgcctc tccttgcaga gggagatgcc gatcgccgtt ggctagcgcg ggcgatggag
     8641 ccggcgatgt cgcggatcag cgacgtctgc cccatcgaca agaacaggat gcggtgatcg
     8701 ccgcgccagg acggcatgtg cgtcatgatg ccgtcctcga cgtgccacga gccataggcg
     8761 cgcgtgacgc cgattttgtc gagatcgtgg tcgatgtagg cccagaacac cggcgtgttc
     8821 catcgtgcca gagcgtagct gcgcgtcagg cgtggaacga tcttggtcac gccatgtcgg
     8881 cgataatcgg gccgcaccca aacggcgccc gaaaacatga tccgtccata gaaatgctca
     8941 gcggaggacg catccacctc gacgcgatcg ccggcggcga cat
//
LOCUS       DASYNS010000333         7240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3203354, whole genome shotgun sequence.
ACCESSION   DASYNS010000333 DASYNS010000000
VERSION     DASYNS010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7240
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3203354"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..980
                     /locus_tag="VGP50_10410"
     CDS             141..980
                     /locus_tag="VGP50_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027834.1"
                     /translation="MAGDPERNIIARRLTGGYRLLVLMAAALIGSGLLGLRGKPGEPL
                     FNSALAQSPTPPLKPKGASPMEIVRNCPAGHRIEFALPATTLYLDIHWLGSGTIIDLF
                     DAYGINCPSGPVKVGRVEFRGAALRLLDLHSGLGEGAGVFRLYAGGDPDLPGRPRVKP
                     SRSTEYHSEPWVEDDTMLKRIPDVRSYRLYYPGASGGPDYMIRITCSGEFSHRECATD
                     DSFDGSSSYEAFYISYHLPQNKLPIPPMSQLYPTDPTTEPGALLQFNAHFRAWLATLK
                     QKP"
     gene            complement(1106..2011)
                     /gene="nudC"
                     /locus_tag="VGP50_10415"
     CDS             complement(1106..2011)
                     /gene="nudC"
                     /locus_tag="VGP50_10415"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEV8027835.1"
                     /translation="MKEPNFYAAGGIDRAGHHRKDKAWLADRLADPSSRFLPVWRSQN
                     LIIAAESARPRAALLARQEIAAEGETVLLSLIEDCAYFVVDLSALEAPLDVLRTKAPA
                     AFTDLRRIGPLLGRLEGALLAYARGIAYWHGRHRFCGICGSATVSEEGGHVRRCTNPS
                     CNAQHFPRTDPAVIMLVHDGERCLLGRQRAWPTGMHSTLAGFVEPGESLEEAVAREVF
                     EETGIGVDEVAYHSSQPWPFPSSIMLGFHARARDREIRVDHSELQDARWFERSFILAH
                     QDDDAFRLPRRDSIARRLIEDWLRR"
     gene            2109..2205
                     /gene="ffs"
                     /locus_tag="VGP50_10420"
     ncRNA           2109..2205
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGP50_10420"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2309..4048
                     /locus_tag="VGP50_10425"
     CDS             2309..4048
                     /locus_tag="VGP50_10425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV8027836.1"
                     /translation="MTEPGAAYRVLARKYRPSRFDALIGQEAMVRTLTNAIATGRIAH
                     AFILTGVRGVGKTTTARILARALNCVGADGKGGPTVAPCGVCEPCRTIAEDRHVDVLE
                     MDAASRTGVDDIRELTDGVRYKPVSARYKIYIIDEVHMLSKNAFNALLKTLEEPPGDV
                     IFIFATTEIHKVPITVLSRCQRFSLRRVPVELLAEHYRGIAVAEGVTAEPAAIALIAR
                     AADGSVRDGLSLLDQAIALGGPSITEDAVRTMLGVADRGLVFDLLEAVLRGDAAGALR
                     RMAALYEGGGDPLMVLQDLLELVHFLTRLKITAGAGEGEPALEGERSRGLALVPKLAL
                     PVLARAWQMLLKGITEAQTAPSPLQAAEMVLVRLAYAADLPAPGDLVKQLTSRADAPS
                     AAPSAPSVPRAAPAAPRFGHSGEASGSAAARLMPEDAAVAPAMPQPQSFLDVVELFDR
                     QREAVLRAHLHAHVHLVHFEPGRIEFRPADGAPRDLANRLGKMLGEWTGMRWVVSVSR
                     EEGAPTLRDQAESRALSLRSEAAHDPLVRAVLEAFPGARIDAVRELVTNEPQAESEPL
                     DGSEARSEGDDVR"
     gene            4045..4368
                     /locus_tag="VGP50_10430"
     CDS             4045..4368
                     /locus_tag="VGP50_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV8027837.1"
                     /translation="MKNIGQMMKQAQEMQAKMAEMQARLDQVEVAGASGGGLVQVTLT
                     GKGDMRQVKIDKSLLDPNEVEVLEDLIRAAFSDAKARVESHMAEEMSKLTGGLKLPPG
                     MKLPF"
     gene            4473..5069
                     /gene="recR"
                     /locus_tag="VGP50_10435"
     CDS             4473..5069
                     /gene="recR"
                     /locus_tag="VGP50_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEV8027838.1"
                     /translation="MSDLDRLIQLLAKLPGLGPRSARRAALFLLKRREVLMEPLAAAL
                     AETAKRIRPCTVCGNLDTLDPCALCRDPRRDMSLVCVVEDVADLWAVERSGAYRGRYH
                     VLGGTLSALDGVGPDDLHIDRLVRRVADGGVREVILAMSATVEGQTTAHYIMDRLAES
                     GTQISRLAHGVPVGGELDYLDEGTLSAALAARQPIKNH"
     gene            5081..5803
                     /locus_tag="VGP50_10440"
     CDS             5081..5803
                     /locus_tag="VGP50_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV8027839.1"
                     /translation="MTMIGDRGTRVDAIADGIYRIATPLTTVPGGFSYNQYLVVDEEP
                     LLFHTGPRRLFPLVREAVGFVLPLGRLRHIAFSHFEADECGALNEFLEASPAAAPLCG
                     KIAAMTSVNDVADRPPRVLGDGESISLGAHEVQWIDTPHLPHAWECGLLFEKRTRTLL
                     CSDLFTCGGAVHPPLTEADILEPSEAFRHQLDYFSHAPNAPAMIERLAELAPTTLALM
                     HGPAWRGDGATLLRALGGRLAH"
     gene            complement(6098..>7240)
                     /locus_tag="VGP50_10445"
     CDS             complement(6098..>7240)
                     /locus_tag="VGP50_10445"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV8027840.1"
                     /translation="AHEASRQGDFERLKAESLQATQAAVLTTAQQISSKLLDDHKREN
                     AEAKKDAEERVRQVNERLVQQVDEIAKAVHQLSGQLQDKARVLDTLWRALSSPGGAGH
                     LAEVGLANTLKSFGLEHGRDFVLQHTTHDEDTGRKLRPDAVVFLPGNSALVIDCKASK
                     FLLEIARAQGSAEEAEAFRNLARTMNQHLRALAEKDYKGAILAAWRQAGRSDEIARIL
                     SVMYLPNEAALEKLNRADPDFLQKARTAQIIPAGPAGLHCAISLASVEINLLRQVENQ
                     ERIVETTQALIDGIAVALGHAGAIGKSIKAAAESFETFTNSVNRRLLPRARSLAKLGL
                     QPNKPLPGNLAAYTIVSSESAIIDAEVAEVAEIEELATRRAAISVR"
BASE COUNT         1141 a         2421 c         2426 g         1252 t
ORIGIN      
        1 ccctctcccc tctgtgggag agggtcgggg tgaggggtcg tccagccgtt cggttatcga
       61 agcaatttcc tgcgcgcgta ggcccttccg tcgctccgcg acgggccgtg tgtgatccgg
      121 gtacggggtt gtagtgaatc atggcaggtg atcccgagcg aaatatcatc gcacgccgct
      181 tgacgggcgg ctatcggctc ctggttctaa tggcggcggc actcatcggc agcggtcttc
      241 ttgggctgcg cggcaaacca ggtgagccgc ttttcaactc ggccctggcg caaagcccga
      301 ccccgccact gaaacccaag ggagcgtcgc cgatggagat cgtccgaaac tgtccggcag
      361 ggcaccgcat cgaattcgct ctccctgcga cgacgctata cctcgatatc cattggctcg
      421 gcagcggaac gatcatcgac ctatttgatg cctatggcat caattgtcca tcaggaccgg
      481 tgaaggtggg tcgggtcgag ttccgcgggg ccgcgctgcg cctactcgac ctccatagcg
      541 gcctcggcga gggtgccggc gtctttcggt tgtacgccgg cggagaccct gaccttccag
      601 gccgtccgcg cgtgaagccg agccgcagca ccgaatatca ttcagaacct tgggtcgagg
      661 acgatacgat gttgaagcgt ataccggacg tgcgctcgta ccgtctttat tatccaggcg
      721 caagcggtgg tcccgactat atgattagaa ttacttgctc aggcgagttt agtcataggg
      781 agtgcgccac agacgatagc tttgatggca gctcgtccta tgaagctttc tacatcagct
      841 accaccttcc tcaaaataag ttgccgatac ctcccatgtc acaactgtat ccgacggatc
      901 cgacaacaga gcctggggcg ctgcttcagt ttaatgcgca tttccgcgcg tggctcgcca
      961 cgttgaagca gaagccgtga ttctcataat caggttgcgg tttccagggc aggttcaaag
     1021 agtgggatga cgacgaggca gcggcgaaca tcaatggcga taatccctct cccacagggg
     1081 agagggattt ttcccaccca ggtcgtcagc gccgcagcca atcctcgatg agacggcgcg
     1141 cgatcgagtc gcggcgcggc agccggaacg cgtcgtcgtc ctgatgcgcg agaatgaagc
     1201 tgcgctcgaa ccagcgcgcg tcctggagct cgctatggtc gacgcggatc tcgcggtcgc
     1261 gcgcccgggc atggaagccc agcatgatcg aggacggaaa cggccagggc tgcgaggaat
     1321 ggtaggcgac ctcgtcaacg ccgataccgg tctcctcgaa gacctcgcgc gccaccgcct
     1381 cttcgaggct ctcgccgggc tcgacgaagc cggcgagcgt cgaatgcatg ccggtgggcc
     1441 aggcacgctg gcggccgaga agacagcgct cgccgtcgtg caccagcatg atcaccgccg
     1501 ggtcggtgcg cgggaaatgc tgggcgttgc atgagggatt ggtgcagcgc cgcacatgcc
     1561 cgccctcctc gctcaccgtg gcgctgccgc agatgccgca gaagcggtgg cggccatgcc
     1621 aataggcgat gccgcgcgca taggcgagga gcgcgccctc gagacgcccg agcagcgggc
     1681 cgatgcggcg gagatcggtg aacgcggcgg gcgccttggt ccgaagcacg tcgagcggcg
     1741 cctccagcgc cgagaggtcg acgacgaagt aggcacagtc ctcgatgagg ctgagcagca
     1801 ccgtctcgcc ttcggcggcg atctcctgcc gtgccagaag ggcggcccga ggcctggcgc
     1861 tttcggcggc gatgatcaga ttctggctgc gccagaccgg caggaagcgg ctcgacggat
     1921 ccgccaggcg gtcggcgagc caggctttgt ccttgcggtg gtggccggcg cggtcgatac
     1981 cgccggcagc atagaaattg ggctctttca tcttgggcgc gacactggca cagggcggcg
     2041 gccgcgggca aggattggcg aggatcggcc ttgccgcggt gccatatgga cctgatatag
     2101 tggccccggg aggctggcag cgggcaggtc cctcgccaac ccggtcaggt ccggaaggaa
     2161 gcagccgtaa cgagtttcga tctgggtcgc cgtccagtct cccgcctcat ctgctcttgt
     2221 cgctgggccg tccaggggga ggccggcgcg ccgccaggga acccccggat gacgacacca
     2281 tgcgccatcg cttctcgatg attttgccat gaccgagccc ggcgccgcct atcgcgtgct
     2341 ggcgcgcaaa taccgcccga gccgcttcga cgcgctgatc ggccaggaag cgatggtgcg
     2401 cacgctcacc aatgccatcg ccaccggccg aatcgcgcat gccttcatcc tcacgggcgt
     2461 gcgcggcgtc ggcaagacga cgaccgcgcg catcctggcc cgcgccttga actgcgtcgg
     2521 cgccgacggc aagggcgggc cgaccgtcgc accttgcggc gtatgcgagc cctgccgcac
     2581 catcgccgag gatcgccatg tcgatgtcct cgagatggac gcggcctcgc gcaccggcgt
     2641 cgacgacatc cgcgagctca cggacggcgt gcgctacaag ccggtgtcgg cacgctacaa
     2701 gatctacatc atcgatgaag tccacatgct ctccaagaac gcgttcaacg cgctcttgaa
     2761 gacgctcgag gagccgccgg gggacgtcat cttcatcttc gcgaccaccg agatccacaa
     2821 agtgccgatc acggtgctct cgcgctgcca gcgcttctcg ctgcgccgcg tgcccgtcga
     2881 gctgctggcc gagcactatc gcggcatcgc cgtggccgaa ggcgtcacgg ccgagcccgc
     2941 ggcgatcgcg ctcatcgccc gcgcggccga cggttccgtg cgtgacggcc tctcgctgct
     3001 cgatcaggcg atcgcgctcg gcgggcccag catcaccgag gatgcggtgc ggacgatgct
     3061 gggcgtcgcc gatcgcggtc tcgtcttcga cctgctcgaa gcggtgctcc ggggcgatgc
     3121 ggccggtgcg ctccgccgca tggccgcgct ctatgaaggc ggcggcgatc cgctcatggt
     3181 gctgcaggat ctgctcgagc tcgtgcattt cctgacgcgg ctcaagatca cggcgggtgc
     3241 cggcgagggc gagccggcgc tcgaaggcga gcgcagccgg ggcctcgctc tcgtgccgaa
     3301 gctcgcgctg ccggtgctgg cgcgcgcctg gcagatgctg ctcaagggca ttaccgaggc
     3361 gcagaccgcg ccgtcgccgc tccaggccgc cgagatggtg ctggtccggc ttgcctatgc
     3421 ggccgacttg ccggcgcccg gcgatctcgt caagcagctc acgtcccgcg ccgacgcccc
     3481 ttctgcggca ccgagcgcgc ccagcgtgcc ccgtgctgcg cctgcggcgc cgcgcttcgg
     3541 gcattcgggc gaggcctcgg gcagcgccgc ggcgcgcctc atgccggaag acgccgccgt
     3601 tgcgcctgcg atgccgcagc cgcagagttt cctcgatgtg gtggagcttt tcgatcgcca
     3661 gcgcgaagcg gtgcttcgcg cccatctcca tgcccatgtt catctcgtgc atttcgagcc
     3721 cggacgcatc gagttccgtc cggctgacgg cgcgccgcgc gatctcgcca accgtctggg
     3781 caaaatgctc ggcgagtgga cgggcatgcg ctgggtggtg agcgtgtcgc gcgaggaggg
     3841 ggcgccgacg ctgcgcgatc aggccgagtc gcgcgcgctc tcgctccgca gcgaggcggc
     3901 gcatgatccc ctggtccgcg ccgtgctgga ggcgttcccc ggcgcccgca tcgacgcggt
     3961 gcgcgaactg gtcacgaacg agccgcaagc tgaatccgag ccgctggatg gcagcgaggc
     4021 gcgcagcgaa ggagacgacg ttcgatgaag aatatcggtc agatgatgaa gcaggcccag
     4081 gagatgcagg ccaagatggc cgagatgcag gcgcgcctcg accaggtcga ggtcgccggc
     4141 gcttcgggcg gcggcctcgt ccaagttacg ctcacgggca aaggcgacat gcgccaggtc
     4201 aagatcgaca agtcgctgct cgatcccaac gaggtcgaag tgctcgaaga cctcatccgc
     4261 gccgccttca gcgacgccaa ggcgcgcgtc gaaagccata tggcggaaga gatgagcaag
     4321 ctcaccggcg gcctcaagct gccgcccggg atgaagctgc cgttctaaga agtcgtcagt
     4381 tttcagttgt cagttttcag gctgtcgatc ccgcgtgcta cgctggcggg tccactgacg
     4441 actgagaact gagaactgac gacttcttcc cgatgtccga cctcgaccgt ctgatccagc
     4501 tcctggcgaa gctgcccggc ctcgggccgc gctcggcgcg gcgcgcggcg ctgtttctgc
     4561 tcaagcggcg cgaggtgctg atggagcctc tcgccgcggc attggcggag acggcgaagc
     4621 gcatccgtcc ctgcaccgtc tgcggcaatc tcgacacgct cgatccttgc gccctctgcc
     4681 gcgatccgcg ccgcgacatg tccctcgtct gtgtcgtcga ggacgtcgcc gatctctggg
     4741 cggtcgagcg ctcgggcgcc tatcgcggcc gctaccatgt cctgggcggc acgctctcgg
     4801 cgctcgacgg cgtcgggccc gacgaccttc acatcgatcg gctcgtgcgc cgcgttgcgg
     4861 atggcggcgt gcgcgaggtg atcctcgcca tgagcgccac cgtcgaaggc cagaccaccg
     4921 cgcattacat catggaccgc ctggccgaga gcggcaccca gatctcgcgg ctcgcccacg
     4981 gcgtccccgt cggcggcgag ctcgactacc tcgacgaagg cacgctctcg gcggcgcttg
     5041 ccgcgcgtca gccgatcaaa aaccactgag gaggcaaccc atgaccatga tcggcgacag
     5101 ggggacgcgc gtcgatgcca ttgccgacgg catctatcgc atcgccacgc cgctcacgac
     5161 ggttcccggc ggcttcagct acaaccagta tctggtcgtc gatgaggagc cgctcctctt
     5221 ccacaccggg ccgcgtcgcc tctttccgct ggtgcgcgag gccgttggtt tcgtgctgcc
     5281 gctcggacgg ctgcgccaca tcgcgttctc gcatttcgag gccgatgaat gcggcgcact
     5341 caacgaattc ctcgaggcgt caccagctgc cgcgccgctc tgcggcaaga tcgcggcgat
     5401 gacctcggtg aacgacgttg ccgatcgtcc gccgcgcgtg cttggcgatg gcgagagcat
     5461 cagcctcggc gcgcatgagg tgcagtggat cgatacgccg catctgcccc atgcctggga
     5521 atgcggcctc ctcttcgaga agcgtacgcg cacgcttttg tgcagcgatc tcttcacgtg
     5581 cggcggcgcc gtgcatccgc cgctcaccga ggcggacatc ctcgagccga gcgaagcttt
     5641 tcgccatcag ctcgattact tctcccatgc gccgaacgcc ccggcgatga tcgagcgtct
     5701 cgcagagctc gcgccgacga cgctcgcgct catgcacggc ccggcgtggc gcggcgacgg
     5761 cgcgaccctt ctgcgagcgt taggcgggcg gctggcgcac tgaactcttc cctctcccgc
     5821 accgcgctac ggggatttac acatctggca aagctgctcc ctctccgccc ctcaggggcg
     5881 gagagggtca gggtgaggtg gggcgcgtcg cgtccgagcg ctgcagaacg catccgatag
     5941 cgacgctcca atcgggaaat cttgttcccg accaaggcat caacattccg aatttgcggc
     6001 agcgccccac ctcaccccgg ccctctacgc ccctgagggg cggagaggga gtcatgcgcg
     6061 attgtgaatc cccgagcgcg ccgcgggaga gggtttgcta tcgcacggag atcgccgcgc
     6121 gtcgcgtcgc gagttcttcg atctcggcga cttccgcgac ctcggcgtcg atgatcgcgc
     6181 tctcgctgct cacgatggtg taggcggcga gattgccggg cagcggcttg ttgggctgga
     6241 ggccgagctt ggcgagactg cgggcgcggg gcaggaggcg gcggttgacc gagttggtga
     6301 aggtctcgaa gctctcggcg gccgccttga tgctcttgcc gatggcgccg gcatgaccca
     6361 gcgccacggc aatgccgtcg atgagcgcct gggtcgtctc gacgatgcgt tcctggttct
     6421 ccacctggcg cagcaggttg atctcgaccg aggcgagcga gatcgcgcaa tggaggccgg
     6481 ccgggccggc ggggatgatc tgcgccgtcc gcgccttctg aaggaagtcg ggatcggcgc
     6541 ggttgagctt ctcgagcgcc gcttcgttcg gcagatacat caccgagaga atgcgcgcga
     6601 tctcgtcgct gcgaccggcc tgccgccagg ccgcgaggat ggcgcccttg tagtccttct
     6661 ccgccagcgc gcgcagatgc tggttcatgg tccgcgccag gttgcggaag gcttcggcct
     6721 cttcggccga gccctgggcg cgcgcgatct cgagcaggaa cttggacgcc ttgcaatcga
     6781 tgaccagcgc gctgttgccg ggcaggaaca cgacggcgtc gggtcgcagc ttccgcccgg
     6841 tatcctcgtc atgggtggtg tgctgcagca cgaagtcgcg gccgtgttcg aggccgaagg
     6901 atttgagggt attggcaagt cccacctcgg cgagatggcc ggccccgccc gggctcgaca
     6961 aggcgcgcca tagcgtgtcg agcacgcggg ccttgtcctg aagctggccc gagagctggt
     7021 gcaccgcctt ggcgatctcg tccacctgct gcaccagccg ctcattcact tgccgcacgc
     7081 gctcctcggc gtcctttttc gcctcggcat tctcgcgctt gtggtcgtcg agcagcttcg
     7141 aggagatctg ctgcgccgtc gtcaggacgg ctgcctgtgt cgcctgcagg ctctcggcct
     7201 tcaaccgctc gaaatcgccc tgccgcgacg cttcatgggc
//
LOCUS       DASYNS010000334        12599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3047111, whole genome shotgun sequence.
ACCESSION   DASYNS010000334 DASYNS010000000
VERSION     DASYNS010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 12599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12599
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3047111"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1251
                     /locus_tag="VGP50_10450"
     CDS             163..1251
                     /locus_tag="VGP50_10450"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEV8027841.1"
                     /translation="MPTVFDLALPLIRRLPPELAHRTTIHALRAGLAPRRRRVDPPSL
                     ALRLWGRDFANPVGLAAGFDKNAEAPDALLRFGFGFVEVGTVTPRPQAGNPRPRLFRL
                     PGDEALINRLGFNNDGLPAATARLTARRGRGGIVGANIGKNRETLDDTADYVQGVTAL
                     APLVDYLVVNVSSPNTPGLRELQRKNRVTALLEKLLAARASVMPSNPPPLLVKIAPDL
                     TAAERADLAAAALATRIDGLIIANTTVARPATLVSPEAHEPGGLSGRPLFAPSTALLA
                     EMYRLTSGKVPIIGVGGIASGADAYEKIRAGASLVQLYTALVYRGPGLVQRIKLELAA
                     LLVRDGFASLAEAIGSAAHSPRTPAHGR"
     gene            1279..2151
                     /locus_tag="VGP50_10455"
     CDS             1279..2151
                     /locus_tag="VGP50_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEV8027842.1"
                     /translation="MTLRLLGGIGEIAPQYDGFILDLWGVLHDGTAPFPGVLDALAHL
                     RSAGKRVAILSNAPRRATLVAERLREIGIPPALYDHIYSSGEEAWRHLQQRDDAFHAA
                     LGRVCYHIGPARDDNMLAGIGLTRVADIDAADFILNTGPGRWEETVADYEPMLVRAHA
                     RDLPMVCANPDLVVMHQNHRAICAGALAQRYEEMGGRVRWHGKPFAPVYETCFALLGI
                     ADRRRILAVGDSLRTDIAGAATVGIDSVLVAGGIHGEEFGIAEHEIPDLERLAAGVAA
                     SGHRPNAVIARFRW"
     gene            complement(2314..2529)
                     /locus_tag="VGP50_10460"
     CDS             complement(2314..2529)
                     /locus_tag="VGP50_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027843.1"
                     /translation="MTAKKRLFVSAEETATSEAQTLRVLEFIAHYLDRIDDHLERLAA
                     QVESGQAGGARIVQGLEQITKALRSKT"
     gene            complement(2526..3077)
                     /locus_tag="VGP50_10465"
     CDS             complement(2526..3077)
                     /locus_tag="VGP50_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027844.1"
                     /translation="MQHPLGADTQAKIQTAVAGRPEVVRPTIEPKKIIVSRVDNSETS
                     EVETYSHADVVARKWVAIGNTAERLSLHVVVVFGDLFVWRSSLSVSREDAAGKHFIQR
                     AIQADWEFNAGVRPRWWPNDLEAERIWGVHCREDRENGRAELARVRLERYDLKPIALA
                     ELPPRDSWSSAIILSPYNRRNGR"
     gene            3215..3835
                     /locus_tag="VGP50_10470"
     CDS             3215..3835
                     /locus_tag="VGP50_10470"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEV8027845.1"
                     /translation="MVDIEPGNREGPPARLKDRPRDIVVIGASRGGVEALKDIVAVLP
                     PGLQSALLIVLHIDPTLTSRLPGILSSSGPLPAEHGRNGMHIERGRIYVAPPNHHMTV
                     GPIGFIGLNRDPKENYTRPAADPLFRSAASVYGSRVIGIVLTGGGRDGTKGLIAIEQA
                     GGLAIVQDPDEARDRSMPTSALVHDNPDLCLPLSEIAGVIMRLSPT"
     gene            3944..4501
                     /locus_tag="VGP50_10475"
     CDS             3944..4501
                     /locus_tag="VGP50_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein B"
                     /protein_id="HEV8027846.1"
                     /translation="MLIQALTSCLVRPSPRRPERRGTGQHRQSARVAPATPAPVSIEK
                     ILTEAFLKDLHRRMCGAVWKWAGQYRRSDKNIGSPCYQVPMDMRMLLGDAVTWRTAKV
                     YGPDEFAVRFAHRLVAIHPWPNVNGRHSRLTADLLAAALGGAPFTWGGGADLALKGGT
                     RDHYITALRRADAGSMGELIAFARS"
     gene            complement(4643..5893)
                     /locus_tag="VGP50_10480"
     CDS             complement(4643..5893)
                     /locus_tag="VGP50_10480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02679 family protein"
                     /protein_id="HEV8027847.1"
                     /translation="MSNDADARLRRLLGGDMLASLRKRLRRHFERAEPSTSSGAVRLG
                     RLSAEEREALASLMGRPPRFSNSMAVDIDAIDTALQRAGIAGSLREALERLDGAIVHA
                     ATARSEREARWSAIAVAARHPGLATCLRTPAGLGLLKRLSRQDHVAAAHLREGADAVL
                     QRLPAHGLPRAQLAAETLGDAHALDAGEAMATLVLTAWRMHDSPSEGDIASNPDESRK
                     EERARDIWARAGVLVNELAKPALFLNLPLVGDDFWLGVPGEPSYASLRSLVRTPPAFA
                     VAGRQVYVCENPNLLAIAADGLGRRCAPMVCTDGMPAAAQRILLTELARAGARLLYHG
                     DFDWPGVRIANHVVRAYGAQPWRFGVADYEDAVPHSPSQRQTLAGPSVAASWNAELAP
                     RMQMHGIAIPEEGLAASLLEDLRR"
     gene            complement(5890..10065)
                     /locus_tag="VGP50_10485"
     CDS             complement(5890..10065)
                     /locus_tag="VGP50_10485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02680 family protein"
                     /protein_id="HEV8027848.1"
                     /translation="MDDLIAPVEIVPGRPALPEPTRLRWQPLRVGLIELFHYDSEEFW
                     FRDGHLLLRGNNGTGKSKVLALTLPFLLDAQLRPSRIEPDGDPDKRMAWNLLMQSYER
                     RVGYAWIEFGRRTEHGLPRYLTLGAGLSAVAARNDVDSWFFIVEGDESGPRLGADLSL
                     IGKHRTVLTRERLREEIAGRGGQTFETAASYRRAVDDRLFRLGPKRYDALIDTLIQLR
                     QPQLSRRPDESRLSIALTEALPPLAIELVGDVAEALNQLEDDRRQIEEFQQLSQAVDR
                     FDQRYRFYAGILTRRQARELRQSQTDFDNASRERNDALAKLVEAKDIELQCQGAYTQA
                     DLALAGGRTRLETLQSDPTMQDANRLEQRQQDMKRRQDDAQTLAETQRRTGERLARED
                     HAVEQCVTHAREVEEELGAARLDCASGAKEVGLTAPMGEHPFNRLEPIPLSEMPAGDF
                     EAAQGALRRMLAARREEVALVRRRQTVFADAMAVTERRRQAMSDKQQELDVAARRRAE
                     ADAAVEREGVALIEAWDTYSAGLRQLRGDMAVPLSALREWVATPDGDNPVQLILQALQ
                     QQAALRLASLQHEIDIEGAGLRDERAALEAERDAVAAGKDGVPPMPYTRSADARCDRP
                     GAPLWQLIDFRDHVDALSRAGIEAAIEASGLLDAWVTPAGSLETPEGRVLRYDSQVVA
                     RARVSTASLADWLCASVAATNPVPAAFVDLLLAGIAGGEDDPGDVEAWVSPAGQFRLG
                     TLAGRWAKPQAVYIGFAARAAARANRLDEIAVRLKELAELQTLLDARSEALAQDRRQA
                     DEEWRAMPSDTPLRQAGLAVRLAAVAFTEAKQRLDQAATAWREAEQSAATARLTLERD
                     AADLNLPAAPDVLTAVESALGRLNDSHYRLAQAVREYRRAWPDLLRQRARAVEAREEL
                     KRSDEQLADARRQAEEARNRFEVLREAVGVKVDSLREQLAAARGAADAAEKALKKASE
                     NLRRAGESRAIAGKDAEAASTLLDQRNEARANAVMRLQRFAESGLLSSALPGIEVPDT
                     RVAWTIDPALGLARRAEQTLAQVKDDDGTWTRTQRHIAEDLQELQRALSALGHQAQSE
                     PSDWGFIVRIIYQNRPERPDVLVARLGEEIAERGELLTAREREVLENHLQAEIAAEIQ
                     RLLQSAEKQLLAVNKELHKRPTSTGVRYRLQWLPLTEEQGAPVGLEAARRKLLNTSAD
                     LWSAEDRRAIGLMLQQRITAERERAEVASNRDGGGTLIDQLARALDYRQWHQFRVQRF
                     QDGQWRKLSGPASSGERALGLTVPLFAAVSSFYGQSTYQHAPRLILLDEAFVGIDDAA
                     RAHCMGLIREFDLDFVMTSEREWACYAELPGVSICHLQRREGIDAVHVSRWTWDGRAK
                     RREEDPDRRFASS"
     gene            complement(10071..11315)
                     /locus_tag="VGP50_10490"
     CDS             complement(10071..11315)
                     /locus_tag="VGP50_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02678 family protein"
                     /protein_id="HEV8027849.1"
                     /translation="MAGSFAIDDLQQRYQREEFRIAVRALLMQPLMSPAHEDFPAIRR
                     QADKLREWFARETGWIVHIGRDGARLYKRPADLGDSTRGLPGYDRRRYVLLCLACAVL
                     ERADPQITLRLLGDRLLLLASEPALTARGFSFTLSTAAERRDLVTICRTLLRVGVLQR
                     VAGDEESFVLGTGGQADALYDVQRRALAGMLAAVRGPSTWTPENAPATLDDRLRALTA
                     EHVADSEEGRRAALRHRLARRLIDDPVVYVDALDPEAQAYFANQRGVMAARLCEATGL
                     TAEQRAEGTALTDEAGILTDVALPAEGTEAHATLLLTEHLAQLVRGKPAQTAMTGEDD
                     VLSFLRDAQERYGRYWRKSAREPGAERELAEIALDRLQKLQLVAREQGIVRPRPALAR
                     FSLGETDFRRKRPQADLLSRSR"
     gene            complement(11327..>12599)
                     /locus_tag="VGP50_10495"
     CDS             complement(11327..>12599)
                     /locus_tag="VGP50_10495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR02677 family protein"
                     /protein_id="HEV8027850.1"
                     /translation="SSLSDFYKARFLYRLSLGGEAVEAGLAAFVQMLRRRAELQTVAL
                     EDIASRLLALKTLVENDVESAIGDVAKIHETLRDLVRVFEGLAENAQAFMAGVARSIE
                     LQQAEVNAVVNYKRRLIDYLERFMGDLVRRSDTIARTILAVSPDIDPILRSVADREAR
                     DAAPGDSSDQADERDRRWQEWRERWKGLSGWFLPAGHDPPQAELLRARARAAIPQLLN
                     AVAALNERRSGRSDRSADFRALAGWFLDCEDDAAAHRLARAAFALNAARHFSLNPQVD
                     DDLPASTRWADAPPLQIHPRLREYGEVAPRGPLASVRDRREDRARLERELTEESQQVE
                     AARQRLATGEPIRLSEIGHLDSHAFGLFLSLLGEALGEQCGPDTAVERLTGDGLLRVR
                     LDPLAAASHARIKTSAGIFAGRDHLITITRT"
BASE COUNT         2011 a         4282 c         4135 g         2171 t
ORIGIN      
        1 ccacgccgcg tgcttgacgc cggcgacgag cgccggcgcc tccggcaggg cgacgggttt
       61 cccggctgcg gagctcatcc acgcccggta taaactgcgg cagggttaac gtcacgcctt
      121 gcccttgtgg tcgcgggtgc gcttgcggta gcgttctccg cgatgcccac cgtctttgac
      181 ctcgcgctgc cgttgatccg ccgcttgccg ccggagctgg cgcaccggac gacgatccac
      241 gcgctccgcg ccgggcttgc gccgcggcgt cggcgcgtcg atccgccgtc gcttgcgctc
      301 aggctgtggg gacgcgactt cgctaacccg gtcggtctcg cggcgggctt cgacaagaac
      361 gcagaggcgc ccgatgcctt gctgcgcttc ggcttcggtt tcgtcgaagt cgggacggtg
      421 acgccgcgac cgcaagccgg caacccgcgt ccgcggctct tccgcctgcc cggggacgaa
      481 gcgctgatca accgtctcgg cttcaacaac gacggactcc cggccgcgac cgcgcggctg
      541 accgcgcggc gcgggcgcgg cggcatcgtc ggcgccaata tcggcaagaa ccgcgagacc
      601 ctcgacgata ccgccgatta cgtgcagggc gtgaccgcgc tggcaccgct tgtcgactat
      661 ctcgtcgtga acgtgtcctc gccgaacacg ccgggcttgc gcgagctgca gcgcaagaac
      721 cgggtcaccg cgctcctcga gaagctgctc gcggcgcgcg cctcggtcat gcccagcaat
      781 ccgccgccgc tcctggtgaa gatcgcgccc gatctcaccg cggccgagcg cgcggatctt
      841 gcggcggccg cgctggcgac gcgcatcgac gggctcatca tcgccaacac gacggtcgcg
      901 cggccggcaa cgctcgtgag ccccgaagcg catgagcccg gcggcctgag cggccgcccg
      961 ctcttcgcgc cgtcgacggc cctcctcgcc gagatgtacc ggctcacttc gggaaaagtg
     1021 ccgatcatcg gcgtcggcgg catcgcctcg ggcgccgacg cctatgagaa gattcgcgcc
     1081 ggcgcgagtt tggtccagct ctacacggcg ctcgtctatc gcggccccgg actcgtccag
     1141 cgcatcaagc tcgagctcgc ggcgctcctc gtccgcgacg gcttcgcctc gctcgccgaa
     1201 gcgatcggca gcgccgccca ttcgccgcgg acgcccgcgc atgggcgtta ggagcctgtc
     1261 tcctcgccga catcgcgcat gacgcttcgc ctgctcggcg ggatcggcga gatcgcgccg
     1321 cagtatgacg gcttcatcct cgacctctgg ggcgtgctgc atgacggcac ggcgccgttt
     1381 cccggcgttc tcgacgcgct ggcgcacctc agatcggccg gcaagcgcgt cgccatcctg
     1441 tcgaacgcgc cgcggcgcgc gacgttggtg gcggagcgcc tgcgcgagat cggcatcccg
     1501 ccggcgctct acgatcacat ttattcctcg ggcgaagaag cctggcggca tctgcagcag
     1561 cgcgacgacg ccttccacgc cgcgctcggc cgcgtctgct accacatcgg tcctgcgcgc
     1621 gacgacaata tgctcgcggg gatcggcctg acgcgcgtcg ccgacatcga tgccgccgac
     1681 ttcattctca acaccggtcc cgggcgatgg gaggaaacgg ttgccgatta cgagccgatg
     1741 ctggtccggg cgcacgcccg cgatctgccg atggtgtgcg ccaatccgga cctcgtcgtg
     1801 atgcaccaga atcaccgcgc catctgcgcc ggcgcgctgg cgcagcgcta cgaagagatg
     1861 ggcggccgcg tgcgctggca cgggaaaccc ttcgcgcccg tctatgagac gtgcttcgcc
     1921 ttgctcggca tcgccgaccg ccggcgcatc ctcgccgtcg gcgacagcct gcgcaccgac
     1981 atcgccggcg ccgcgaccgt cggcatcgac agcgtgctgg tcgccggcgg tattcacggc
     2041 gaagaattcg gcatcgccga gcacgaaata cccgatctcg agcggctcgc tgccggcgtc
     2101 gcggcgagcg gccatcgacc gaatgcggtg atcgcgcgct ttcgttggtg atcagcgatc
     2161 ggcgcgattg aggatcgcac cgagactctc ggagcttgcc gcaacgctga aaaatgcggc
     2221 gcccgcggcg agcgctacgc cctggcgcac gtcacggcga gcacctcgaa acgtggaatt
     2281 cgttgacggg agtggagcgt gccgagctcg gattcaggtc ttcgagcgca gggcctttgt
     2341 tatctgctcc aacccctgaa caatcctcgc tccgcccgcc tggcccgact cgacctgcgc
     2401 tgcaagtctt tcaaggtggt cgtcaatccg gtccagatag tgagcaatga attcgagcac
     2461 gcgcagcgtc tgggcttccg acgtggcggt ctcttcagca gatacaaata gtcttttctt
     2521 ggcggtcatc gtccgttcct tcggttgtac ggactcagga ttattgcact agaccacgaa
     2581 tcgcgcggcg gcagttcggc caaggcaatc ggtttcaggt cataacgctc caatcttacg
     2641 cgggccaact cggcgcggcc gttctcacgg tcttcgcggc aatgcacgcc ccaaatgcgc
     2701 tcggcctcca gatcgttcgg ccaccatcgc gggcgcacgc cggcgttgaa ctcccaatcg
     2761 gcctggatcg ctcgctggat gaaatgtttc cctgccgcgt cctctcggct caccgagagg
     2821 gacgaccgcc acacgaagag atcgccgaac acgacgacaa catgaaggct cagcctctct
     2881 gccgtgttgc caatcgcaac ccacttccgc gccaccacat cggcgtgcga ataggtttcc
     2941 acctccgacg tctcggaatt gtcgacgcgg ctgacgatga tcttcttagg ctcaatcgtc
     3001 ggccgcacga cctcgggtcg tccggcgaca gccgtctgaa ttttggcctg ggtgtcggcc
     3061 ccgagggggt gttgcatgtc ctcaccgatt ttccaggggc gtggccttcc agcgtcaatt
     3121 tgcacggggg gggggtggag ataaggttcg gaatctacct aacggggcat aaatcgcggg
     3181 atcaaccccg gtgggtcgcg aggaggggaa tcggatggtc gacatcgagc ctgggaatcg
     3241 agaaggccct cccgcacggc tgaaggatcg tccgcgagac attgtggtca ttggagcctc
     3301 gcggggaggc gtcgaggccc ttaaggatat tgtcgcggtt ttgccgcccg gcctgcagtc
     3361 cgcgctcctc atcgtgcttc acatcgaccc gacgcttacg agcagacttc ctggcatcct
     3421 gtccagctcc ggcccacttc cggccgagca cggaagaaat ggcatgcaca ttgagcgcgg
     3481 ccgcatttac gtggcgccgc cgaaccatca catgacagtc ggcccgatcg gcttcatcgg
     3541 cctgaaccga gatcctaagg agaattacac caggccggcc gccgatccac tcttccgatc
     3601 ggcggcgagc gtatacggct cgcgcgtcat cggaatcgta ctgacggggg gcggtcgcga
     3661 tggcacaaaa gggctcattg cgatcgagca ggccggcggc ctcgccatcg tgcaggatcc
     3721 ggacgaggct cgggatcgca gtatgccgac gagcgctctt gtccatgata acccggacct
     3781 ctgcttgcca ttgagcgaga tagcgggcgt catcatgcgg cttagcccaa cttgagccgt
     3841 tgggaggggg ttggcgagtg ttgggcgggg ccggaggcca gattaggcac ttgggtgacg
     3901 gcggccgatt cggtcgtgta gtgccgatct tgggtaagtc aaaatgctca tacaggcttt
     3961 gacatcctgc ttggtgcggc cctccccgcg ccgacctgaa cgccgcggaa cgggacaaca
     4021 tcggcaaagc gcgcgcgtcg ctcctgcgac gcctgcgcca gtttcgatcg aaaagatcct
     4081 gaccgaggcc ttcttgaagg atcttcaccg ccggatgtgc ggcgccgtct ggaagtgggc
     4141 cggacagtac cggcgcagcg acaagaatat cgggtcgccc tgttatcagg tgccaatgga
     4201 catgcgcatg ctgctcggcg acgcagtgac atggcgcacc gcgaaggtct atgggccgga
     4261 tgagttcgcc gtgcggttcg cgcatcggct ggttgccatc catccctggc cgaatgtgaa
     4321 cggccgccat tccaggctga cggcggacct tctcgccgcc gcgcttggcg gcgcgccatt
     4381 cacctggggc ggcggcgccg atctcgcgct gaagggtggg acgcgagacc actatatcac
     4441 agcgctccgg cgtgccgacg cgggctccat gggagagctg atcgccttcg ctcgttcgtg
     4501 agctcgccat ccgcggtctt tggtcgcgaa ccgcggggct ctatccgacc gcgcaattgt
     4561 ggcatcaccg acgccgtcca cgggggtgat gaagccggca ggcataggat ggctcggttc
     4621 cgcggcgacg ggtcgacggc agctaacgcc gaaggtcctc caacagcgag gcggcgaggc
     4681 cctcctctgg gattgctatg ccatgcattt gcatccgcgg tgccaactct gcgttccagg
     4741 aggctgcgac tgatgggccg gcgagcgttt gtctctgact tggagagtgg ggcactgcgt
     4801 cctcgtaatc ggccacaccg aagcgccacg gttgcgcgcc atacgcgcgc accacgtggt
     4861 tggcgattcg cacgcccggc cagtcaaagt ccccatgata gagcagtcgg gcacccgccc
     4921 gcgccagctc ggtcagcaag atgcgctgcg cagcggccgg catgccgtcg gtacacacca
     4981 tcggcgcaca gcgccgacca aggccgtcgg ccgcgatcgc gagcaagttt gggttctcgc
     5041 agacatagac ctgacggccg gcgacggcaa atgccggcgg cgtacggacc agggagcgca
     5101 aggacgcata gctcggctct cccggcacgc cgagccagaa gtcgtcgccc acgagcggga
     5161 ggttcagaaa caacgccggt ttagccagct cattgaccag cacgccggcg cgcgcccaga
     5221 tgtcgcgtgc ccgctcctct tttcggcttt cgtccggatt gctcgcgata tcgccctcag
     5281 agggcgaatc gtgcatccgc catgcggtca ggacgagggt cgccatcgct tcgccggcgt
     5341 cgagcgcgtg ggcatcgccc agcgtctccg ccgccagttg ggcgcgcggc aggccgtggg
     5401 cggggagccg ctgcaaaacg gcatcagcgc cctcgcgcag atgcgccgcc gccacatggt
     5461 cctgtcggct gagccgtttc agaagtccca gtcccgccgg cgtccgcagg caagtggcga
     5521 gaccggggtg ccgggcggcc acggcaatgg ctgaccagcg cgcctctcgc tcggaacgcg
     5581 ccgttgcggc atggacgatc gctccatcga gccgctccag cgcttcgcgc aacgagcccg
     5641 cgatcccggc tcgctgcaaa gccgtgtcga tggcgtcgat gtcgaccgcc atggaattgg
     5701 aaaaacgcgg cggtcgcccc atcaacgaag ccagcgcctc ccgttcctct gccgagagtc
     5761 gcccgagccg aacggcgccc gatgacgtgg acggctccgc ccgttcgaaa tgacgacgca
     5821 atcgcttgcg caaggaggcg agcatgtcgc cgccaaggag acgtctgagc cgcgcgtcgg
     5881 catcgttgct catgacgaag cgaaccgacg gtctggatcc tcctctcgac gcttggcgcg
     5941 gccgtcccac gtccagcgcg aaacatggac ggcatcgatg ccttcgcgcc tctgcagatg
     6001 acagatcgag acaccgggca attcggcata gcaagcccac tcgcgctcgc tcgtcatgac
     6061 gaagtcgaga tcgaattcgc ggatcaggcc catgcaatgc gcccgagcgg catcgtcgat
     6121 gccgacaaag gcctcgtcga gtaggatcag ccgcggcgca tgttgatagg tgctctggcc
     6181 gtagaagctt gagacggcgg cgaagagggg aacggtgaga cccaacgcgc gctcgccgct
     6241 ggacgccggt ccggacagct tgcgccattg tccgtcctga aaacgctgga cgcggaattg
     6301 gtgccattgg cggtaatcga gggcacgggc gagctggtcg atcagtgtcc cgccgccatc
     6361 ccgattcgag gctacctcgg cccgctcgcg ctctgcggtg attcgctgct gcagcatcag
     6421 tccgatggcg cgccggtcct ccgcagacca tagatccgcg ctggtgttga gcagtttccg
     6481 ccgagcggct tcgaggccga cgggcgcgcc ctgctcctcg gtcagcggca accactgcaa
     6541 gcgatagcgc acgccggtgg aagtgggacg cttgtgcagc tctttgttga ccgcgagcag
     6601 ctgcttttcc gcgctctgaa gcaggcgctg aatttcggcg gcaatctccg cctgcagatg
     6661 gttctccagt acttcgcgtt cgcgcgccgt gagcagttcg ccgcgctcgg caatctcctc
     6721 gccgagccgc gcaacgagga cgtcgggccg ctccggccgg ttttggtaga tgatccgaac
     6781 gataaagccc cagtcgctcg gctcggactg ggcctgatgg ccgagagcgc tcagtgcccg
     6841 ctgcagctcc tggaggtctt cggcgatgtg ccgttgcgtg cgcgtccagg tgccgtcgtc
     6901 atccttgact tgcgcgagcg tttgctcggc acggcgcgcg agaccgagcg cgggatcgat
     6961 cgtccaggcg acgcgcgtgt cgggcacctc aatgcctggc aaggcggaag acagcaggcc
     7021 gctctcggcg aagcgttgca agcgcatcac ggcattggct cttgcctcgt ttcgctgatc
     7081 gagtagcgtc gacgcggcct cggcgtcttt gccggcgatg gcacgacttt cgccggctcg
     7141 gcgcagattc tcgctggcct ttttgagtgc tttttccgcc gcgtccgcag cgcctcgcgc
     7201 ggcggcgagc tgctcgcgta agctgtcgac cttgacgccg accgcctcgc gcagcacctc
     7261 gaagcgattg cgcgcctctt ctgcttggcg acgagcgtca gcaagctgct cgtcgctcct
     7321 cttaagctct tcccgcgcct ccaccgcgcg agcacgttgc cgtagcaggt cgggccaagc
     7381 ccgccggtat tcccggacgg cttgtgcgag ccggtagtgg ctgtcgttga ggcgcccgag
     7441 cgcgctctcg acggcagtaa gaacgtccgg agccgccggc aggttcagat cggctgcgtc
     7501 gcgctcgagc gtgaggcgcg cggtcgccgc ggactgctcg gcttcgcgcc aagccgttgc
     7561 cgcctgatcc aaccgttgtt tcgcctcggt gaacgctacc gctgccagcc tcacggcaag
     7621 gcccgcctga cgcaagggcg tgtccgacgg catggcgcgc cactcttcgt cggcttgccg
     7681 acggtcctgg gcgagggcct ccgatcgcgc gtcgagcaga gtttgcagct ctgccagttc
     7741 cttgaggcgc acggcgattt cgtcgagccg attcgcgcga gcggccgctc gggcggcaaa
     7801 tccgatgtag acggcctgag gcttggccca acgcccagca agagtcccaa gccgaaactg
     7861 ccctgccggc gagacccacg cctcgacgtc gcccggatcg tcttcgccgc cagcgatccc
     7921 ggcgagcagc agatcgacga aggcggctgg cacagggttc gtggcagcta cgctcgcgca
     7981 tagccaatct gccagcgagg cggttgatac gcgggcgcgc gcgaccactt ggctgtcgta
     8041 gcgcaagacg cggccttccg gcgtctcgag cgagccggcc ggcgtgaccc aggcgtcgag
     8101 gagcccggac gcttcgatcg cggcttctat gccggcgcgc gaaagagcgt cgacatgatc
     8161 gcggaagtcg atgagttgcc acagcggagc accggggcgg tcgcatcgcg catccgcgct
     8221 gcgcgtgtaa ggcatgggcg gaacgccgtc tttgcccgct gcgacggcat cgcgctccgc
     8281 ttccagtgcc gctcgctcgt cccgcagccc cgcgccctcg atatcgatct cgtgctgcaa
     8341 cgatgcgaga cgcagggcag cctgctgctg cagcgcttgc aagatcagtt gcaccggatt
     8401 gtcgccgtcc ggcgtcgcga cccattcgcg gagagcggag agcggcacgg ccatgtcgcc
     8461 gcgcaactgc ctcagtccgg cgctatatgt atcccaggcc tcgatgagcg ccacgccctc
     8521 gcgctccacg gcagcatccg cctctgcccg acggcgggct gccacatcca gctcctgctg
     8581 tttgtcgctc attgcttggc gacggcgctc ggtgactgcc atcgcatcgg caaagacggt
     8641 ttgtcgccgc cgcacgaggg cgacttcctc ccgccgggcc gcgagcatcc gccggagcgc
     8701 cccttgcgcc gcctcgaaat cgccggcggg catctccgat aaagggatcg gctcgaggcg
     8761 gttgaacgga tgctcgccca tcggggcggt caggcctact tccttggctc cgctcgcgca
     8821 atccagccgc gcggcgccca attcctcttc gacttcccgc gcatgggtca cgcactgctc
     8881 gacggcatgg tcctcgcgcg cgagccgctc gcctgtcctt ctctgggtct ccgccagcgt
     8941 ctgcgcgtcg tcctggcggc gcttcatgtc ctgttggcgc tgttcgagcc gattggcgtc
     9001 ttgcatcgtc gggtccgact gcagcgtctc cagccgtgtc cgtcctccgg ccagcgccag
     9061 atccgcttgc gtgtatgcgc cctggcactg caactctatg tccttggcct ccaccagctt
     9121 ggcgagcgcg tcattccgct cgcggctcgc gttatcgaaa tcagtctgcg actgcctcag
     9181 ctctcgggct tgacgccgcg tcaagatacc cgcgtagaag cgatagcgct ggtcaaaccg
     9241 gtccaccgct tgcgagagct gctgaaattc ctcgatttgg cgacggtcgt cctcgagctg
     9301 gttgagcgct tcggccacgt cgccgacgag ctcgatcgcc aggggcggca aagcttcggt
     9361 caacgcgatc gagagccggc tttcgtccgg tctcctggat agctgcggct ggcggagttg
     9421 gatcaacgtg tcgatgagag cgtcgtagcg cttgggccca aggcgaaaga ggcgatcgtc
     9481 gacggcgcgg cggtaggatg cagcggtctc gaaggtctgg ccgccgcgtc cggcgatctc
     9541 ttcgcgcagg cgctcccgag tgagtaccgt gcgatgcttg ccgatgagcg ataggtccgc
     9601 tccgagtctc ggcccgctct cgtctccctc gacgatgaag aaccagctat cgacatcgtt
     9661 gcgggcagcg accgccgaca ggccggcgcc gagggtcaga taacgtggca acccgtgctc
     9721 cgtccgccgc ccgaactcga tccaggcata gccgacgcgt ctctcgtagc tctgcatcag
     9781 cagattccac gccatgcgct tgtcgggatc gccgtcgggc tcgatccgcg aaggccgcag
     9841 ctgcgcgtcg agcaggaacg gcagagtcag tgccaggact ttcgatttcc cggttccgtt
     9901 gttcccgcga agcagcagat ggccgtcgcg gaaccagaat tcttcgctgt cgtagtgaaa
     9961 cagctcgatc agaccgaccc tgagaggctg ccagcgcagc cgggtcggct ccggcagcgc
    10021 cggccgcccg gggacgattt cgaccggtgc gatgaggtca tccatgtcga tcaacgggac
    10081 cggctgagca aatcggcttg cggccgcttg cggcgaaaat cggtctcccc cagagagaag
    10141 cgcgcgagcg ctggccgagg ccggacaatg ccctgctcgc gtgcgaccaa ctggagcttc
    10201 tgcaagcgat caagggcgat ctcggccaat tcgcgctcag cgccgggctc gcgcgccgac
    10261 ttgcgccaat agcggccata tcgctcctgg gcatcgcgca ggaagctcag cacgtcgtcc
    10321 tctccggtca tggcggtctg agccggcttt ccccggacga gctgcgcgag gtgttcggtc
    10381 aagagcaagg tcgcatgcgc ttccgtgccc tcggctggca gcgcgacgtc ggtcaaaatg
    10441 ccggcttcgt cggtgagcgc tgtcccctcg gcacgctgtt ccgccgtcag gcctgtcgcc
    10501 tcgcacagac gggccgccat gacgccacgc tgattggcga aataagcttg cgcttcagga
    10561 tccaaagcgt cgacatagac gacgggatcg tcgatcaacc gtcgcgcaag acgatggcgc
    10621 aacgccgcgc ggcgaccctc ctcgctgtct gccacatgtt cggccgtcaa agcgcgcagc
    10681 cggtcgtcga gggtggcagg cgcgttttcc ggcgtccagg tggacggccc gcgcacagcg
    10741 gccagcatgc ccgccagcgc tcggcgctgc acgtcataaa gggcgtcggc ttggccaccc
    10801 gtgccgagca cgaagctttc ctcgtcgccg gccacgcgct gcaggacgcc gacgcgcaac
    10861 aacgttcggc agatcgtcac gaggtcacgc cgctcggccg ccgtgctcaa ggtgaaactg
    10921 aatccgcgcg ccgtgagcgc cggctcgctc gcgaggagca gaaggcggtc gccgagaagt
    10981 cgcaaggtga tctgcggatc ggcccgctcc aacacggcgc aggccaggca cagcagaacg
    11041 tagcggcggc ggtcataccc cggtagcccg cgcgtggagt cgccgaggtc ggccggccgt
    11101 ttgtaaaggc gcgcgccatc ccgccctata tgaacgatcc agcccgtctc gcgcgcgaac
    11161 cattcgcgta gcttgtcggc ttgccggcgt atggcgggaa agtcctcatg cgccggactc
    11221 atcaacggct gcatcagcag ggcccgcacc gcaattcgaa actcctcgcg ctgatagcgt
    11281 tgctgcaggt cgtcgatggc aaagctgccc gccatgatct cgcgtcctag gtccgcgtga
    11341 ttgtaataag gtgatcgcgg ccggcgaaga tcccggccga agtctttatc cgggcgtggc
    11401 tcgcggccgc gaggggatcg aggcgaacgc gcagcaatcc atcgccggtg aggcgctcga
    11461 cagcggtatc gggtccgcac tgctcgccca gcgcctcgcc cagcaggcta agaaacaggc
    11521 caaacgcgtg cgagtcgaga tggccgatct ccgacaggcg aatcggctcg cctgtcgcga
    11581 gacgctggcg cgcggcctcg acttgctgcg attcctcggt caattcgcgc tccaggcgcg
    11641 cccggtcctc gcgccggtcc cgcacgctcg ccagcggtcc gcgaggcgcg acctcgccat
    11701 attcgcgcaa gcgcggatga atctgtagcg ggggggcgtc cgcccatcgg gtgctggccg
    11761 gcaggtcgtc gtccacctgt ggattgagcg agaaatggcg tgccgcattg agtgcaaaag
    11821 ccgcgcgcgc cagccgatgc gccgcggcat catcctcgca gtcgaggaac catcccgcaa
    11881 gcgcgcggaa gtcggcggag cggtcgctac ggccgctgcg ccgctcgttg agcgccgcca
    11941 ccgcattgag aagctgtggg atcgctgcgc gggcccgtgc tcgaagcaat tcggcctgcg
    12001 gcgggtcatg ccccgccggc aggaaccaac cgctgagacc tttccatctc tcgcgccatt
    12061 cttgccagcg ccgatcccgt tcatctgcct gatccgacga atctcccgga gcagcatccc
    12121 gcgcctcccg gtcggccacc gatcgcaaga tgggatcgat atccggcgag acggccagga
    12181 tggtgcgggc gatagtgtct gagcggcgga caagatcgcc catgaagcgc tcgaggtagt
    12241 cgatcaggcg gcgcttatag ttgaccaccg cattgacctc ggcttgctgc agttcgatgc
    12301 tgcgggcgac gccagccatg aaggcctggg cgttctcggc gagtccctcg aagacgcgga
    12361 caagatcgcg cagagtttcg tggattttcg ccacgtcgcc gattgcggac tccacatcgt
    12421 tctcgaccaa ggtcttgagg gcgagcagcc ggctggcgat gtcttccaat gccacggtct
    12481 gcagctcggc tcggcggcgc agcatctgga cgaaagcggc caggcccgcc tcgacggctt
    12541 cgccgccgag cgatagtcga tagaggaagc gggccttgta gaagtcgctg aggctcgac
//
LOCUS       DASYNS010000335         3704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1330605, whole genome shotgun sequence.
ACCESSION   DASYNS010000335 DASYNS010000000
VERSION     DASYNS010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330605"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..943)
                     /locus_tag="VGP50_10500"
     CDS             complement(155..943)
                     /locus_tag="VGP50_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV8027851.1"
                     /translation="MTDEIKYEVDQGVATITLNRPEVMNAFSTEMLDAWYEALRSAQG
                     DDAVHVIIVTGAGKAFCSGGDIKRMGDRAQSTALDRKTYLAGTIQRIPLLLETIDKPV
                     IAAINGAATGAGMDFALHCDLRYAAESARLGETYVRVGLVPGGGGTWFLPRLVGTAKA
                     LELFWTGDLISAQEAERLGIVNKVLPDAELMAHVRSVALRIAQGPPQSIRFIKRAVYQ
                     GMRIDLRTSLDLISSHFAVVSSTADHKEAVEAWQEKRKPKFTGR"
     gene            complement(955..1995)
                     /locus_tag="VGP50_10505"
     CDS             complement(955..1995)
                     /locus_tag="VGP50_10505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8027852.1"
                     /translation="MTDTLVEESAGRLFADLVSPALVNAAEAGTWPADLWRAVAAAGF
                     PDALAAGAESPGDLSGMADAAAILREAGRHAAPLPLAETMLARWLLAGAGIAAPDGPL
                     TVAPVDHPSGFVLGWEGAIRRVSGRAAGVPWARLAQGIVVCIEDHVALIAPGQAELRN
                     GKSLAGEPRDDLSLDAVVETKRAPAGVDAVLAFRLGALTRSVMMAGALDQVLALTVQY
                     ANDRVQFGRPIGKFQAIQQQLSILAENVAAAGVVAGAAVEAAVGKGDLGFAVAVAKSR
                     IGEAAGKVAEIAHQVHGAIGFTHEHRLHHLTRRLWSWRDEFGVESEWSSELGRLVASR
                     GGGGLWPLLTGW"
     gene            complement(1992..3146)
                     /locus_tag="VGP50_10510"
     CDS             complement(1992..3146)
                     /locus_tag="VGP50_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8027853.1"
                     /translation="MAGVFRFDPLTLPPECESLRQDVRAFLVEEKAAGRWRAGGDFSQ
                     GHSPEFSRRLGARGWIGITWPKKYGGQEKSFLERYVLTEELLAAGAPVGAHWVADRQS
                     GPLILRFGSESQREQILPRITRGECYFCIGMSEPDSGSDLASIRTKAVPVEGGWQLDG
                     RKIWTSNAHKAHFMIALCRTGAVDSADRHKGLSQLVVDLKSPGISIRPIKNLAGAEHF
                     NEVLFESVFIPADRMIGAPGDGWKQVTSELGYERSGPERFLSTFRLLTEFVRRAGPSP
                     DEREAEAIGRFVAHLATLRRMSLSVAGMLQRGETVNLEAAVVKDLGTNLEKEIPEIVR
                     LVARRGRSPNQADLMEEALAVGVLNAPSFTIRGGTREILRGIVARGLGLR"
     gene            3324..>3704
                     /locus_tag="VGP50_10515"
     CDS             3324..>3704
                     /locus_tag="VGP50_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027854.1"
                     /translation="MKMTSLRTEFLAFFALCFALASGTAAAQSQPSAMFKVDQFYAGI
                     AVGPIWSQDVSVQLSGPITGSGRLLFNPAGLIGGYAGYELNDQLAAEFEVDWSLYDPF
                     SLSGSFVGPGGRFAGLALDGDFDTV"
BASE COUNT          569 a         1277 c         1268 g          590 t
ORIGIN      
        1 tgtcgacttg gcgatgagcc tcggcctggt cgtcccgaaa ctggtctttg accatgttgc
       61 ggatcgcctg gaacggctcg cggcgaccaa ctcaggctct cggtccgggc gatagaccgg
      121 gtcggccgcg ccgtgcgttt accggtacgg aagctcagcg ccccgtgaat ttcggcttcc
      181 gcttctcctg ccaggcctcg accgcttcct tgtgatccgc cgtcgacgac accacggcga
      241 aatgcgagga gatgaggtcg aggctggtgc ggaggtcgat gcgcattccc tgatagaccg
      301 cgcgcttgat gaagcggatc gactggggcg gaccctgggc gatgcggagc gccacgctgc
      361 gcacatgcgc catgagctcg gcatcgggca ggaccttgtt gacgatgccg agccgctctg
      421 cctcttgcgc cgagatgaga tcgccggtcc agaagagctc gagcgccttc gccgtgccga
      481 cgaggcgcgg caggaaccac gtgccgccgc cgcccggcac caatcccacg cgcacatagg
      541 tctcaccgag ccgcgcgctc tcggcggcgt agcgcaggtc gcagtggagc gcgaaatcca
      601 tgccggcgcc cgtcgccgcg ccgttgatgg cggcgatcac cggcttgtcg atggtctcga
      661 gcaagagcgg gatgcgctga atcgtgccgg cgagataagt cttgcggtcg agcgccgtcg
      721 actgggcgcg gtcgcccatg cgcttgatgt cgccgccgct gcagaaggcc ttgccggcgc
      781 cggtgacgat gatgacgtga acggcatcgt caccttgagc gctacgcagc gcctcgtacc
      841 aggcatcgag catctcggtg ctgaaggcgt tcatcacctc gggacggttg agggtgatgg
      901 tagcgacccc ttggtcgacc tcgtatttga tctcgtcggt catgctgtcc tcgctcacca
      961 ccccgtcagc agcggccaca gaccgccgcc gccgcgcgac gcgacgaggc ggcccaattc
     1021 gctcgaccat tcgctctcga cgccgaactc gtcgcgccac gaccacagcc gccgcgtcag
     1081 gtgatggagg cggtgctcgt gggtgaagcc gatggcgccg tgcacctgat gcgcgatctc
     1141 cgccaccttg cccgcggcct cgccgatgcg gctcttggcg acggcgaccg cgaagccgag
     1201 gtcgcctttc cctacggccg cctcgaccgc ggcaccggcg acgacgccgg ccgccgcgac
     1261 attctcggcg aggatcgaga gctgctgctg gatcgcctgg aacttgccga tgggccggcc
     1321 gaactggacg cggtcattgg catattgcac cgtgagcgcc agcacttggt cgagcgcgcc
     1381 cgccatcatc accgagcgcg tgagcgcgcc caggcgaaaa gcgagcaccg catcgacgcc
     1441 ggcgggcgcg cgttttgtct cgacgacggc atcgagcgag aggtcgtcgc gtggctcgcc
     1501 ggcgaggctc ttgccgttgc gcagctcggc ctgcccaggg gcgatgagcg ccacatggtc
     1561 ttcgatacag accacgatgc cctgggcgag gcgcgcccag ggcacgcccg cggcgcggcc
     1621 ggacacccgc cggatcgcgc cctcccagcc cagcacgaag ccgctcggat gatcgaccgg
     1681 cgcgacggtc agcggcccgt ccggcgccgc gatgccggcg ccggcgagca gccaccgcgc
     1741 cagcatggtc tcggcgagcg gaaggggcgc agcgtgccgg ccggcctcgc ggaggatcgc
     1801 cgcggcatcc gccatgcccg agaggtcgcc gggcgattcg gcgcctgcgg cgagcgcgtc
     1861 ggggaagccg gcagcggcga cggcgcgcca gagatcggcg ggccaggtgc ccgcctcggc
     1921 ggcgttcacc agcgccggcg agacgagatc ggcgaagagc cggccggcgc tttcctcgac
     1981 gagcgtgtcg gtcatcggag gcccaggccg cgggcaacga tgccgcgcag gatctcgcgc
     2041 gtgccgccgc ggatggtgaa ggagggcgcg ttgagcacgc ccacagccag cgcttcctcc
     2101 atgagatcgg cctggttcgg cgagcggccc cgccgcgcga cgagccgcac gatctcgggg
     2161 atctccttct cgagattggt gccgaggtcc ttcaccacgg ccgcctcgag attgaccgtc
     2221 tcgccccgct gcagcatgcc cgccaccgag agcgacatcc ggcgcaacgt cgccagatgg
     2281 gcaacgaatc ggccgattgc ctcggcctcg cgctcgtccg gcgacggacc ggcgcggcgc
     2341 acgaactcgg tgaggaggcg gaaggtgctg aggaagcgct cggggccgct ccgctcatag
     2401 ccgagctcgc tcgtcacctg cttccagcca tcgccgggcg cgccgatcat gcggtcggcg
     2461 gggatgaaca cgctctcgaa cagcacttcg ttgaaatgct cggcaccggc gagattcttg
     2521 atcggccgga tcgagatgcc cggcgatttg aggtcgacga cgagctgcga gaggcccttg
     2581 tggcgatcgg cagaatcgac cgcgccggtg cggcagagcg cgatcatgaa atgcgccttg
     2641 tgggcgttcg aggtccagat cttgcgcccg tcgagctgcc agccgccttc gaccggtacg
     2701 gccttggtgc gaatcgaggc gaggtcggag ccgctgtcgg gctcgctcat gccgatgcag
     2761 aagtagcatt cgccgcgggt aatgcgcggt aggatctgct cgcgctggct ctcgctgccg
     2821 aagcggagga tgaggggtcc gctctggcgg tcggccaccc aatgggcgcc gaccggcgcg
     2881 cccgcggcga gcaactcctc ggtcagcaca tagcgctcga ggaagctctt ctcctggccg
     2941 ccgtattttt tgggccaggt aatgccgatc cagccgcgcg cgccgaggcg gcggctgaac
     3001 tccggcgaat gaccctggct gaagtcgccg cccgcgcgcc agcggccggc ggccttctcc
     3061 tcgacgagaa aggcgcgcac atcctggcgc agcgactcgc attcgggcgg caaggtgagc
     3121 ggatcgaagc gaaagacccc ggccacggtc tccctccccc ggtttttggc catttttccg
     3181 gatcgtagtg accggcatcg cggccgtcaa atgaccgcgc tccgacaggg cgcgacgtca
     3241 catttccgtg cgttgctcga ccgctgggct cccggcacga tgagctgtct caacatcact
     3301 acccaacaat gagaaccatc ccaatgaaga tgaccagctt gcgcacggaa tttcttgctt
     3361 tcttcgcgct ttgctttgcg ctcgcctccg gcaccgccgc cgcgcagagt cagccgagcg
     3421 cgatgttcaa ggtcgaccag ttctatgccg gcatcgccgt cggccccatc tggtcgcagg
     3481 acgtgagcgt ccagttgagc ggtccgatca ccggcagcgg ccggctgctc ttcaatcccg
     3541 ccgggctcat cggcggctat gcaggttatg agctgaacga ccagctcgcg gcggagttcg
     3601 aggtcgactg gtcgctctac gatcccttca gcctctccgg cagcttcgtg ggaccaggcg
     3661 gccgattcgc cggcctcgcc ctcgacggcg acttcgacac ggtg
//
LOCUS       DASYNS010000336         4699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4454174, whole genome shotgun sequence.
ACCESSION   DASYNS010000336 DASYNS010000000
VERSION     DASYNS010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4454174"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VGP50_10520"
     CDS             complement(<1..1258)
                     /locus_tag="VGP50_10520"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV8027855.1"
                     /translation="MLDKTYRPGDVEAVIYAAWEASGAFAAHPDSPAEPYCIMMPPPN
                     VTGSLHIGHALNHTLQDILIRYHRMRGRDALWQPGTDHAGIATQMMVERQLALEQSSR
                     HTLGRAAFIERVWQWKAESGGTITRQLRALGASPDWSRERFTMDEGLSAAVRRVFVRL
                     HKEGLIYRDKRLVNWDPELHTGLSDLEVEFHETKGSLWYFKYPVEGEEGRFITVATTR
                     PETMLGDSGVAVNPKDERYQDLIGKHAILPLVGRRIQIVGDDYADPTAGSGAVKITPA
                     HDFNDFQVGRRHNLALINIFDKDAKLNEATPEAYRGLDRFDARKKIVADLEAQGLVEK
                     IVPHTLMVPHGDRSKVVLEPYLSDQWYCDAATLAKAAIAAVETGRTVFVPKSWENTFF
                     EWMRNIQPWNISRQLWWGHQIPAWYGP"
     gene            complement(1266..2009)
                     /locus_tag="VGP50_10525"
     CDS             complement(1266..2009)
                     /locus_tag="VGP50_10525"
                     /inference="COORDINATES: protein motif:HMM:NF022155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEV8027856.1"
                     /translation="MPQAESQPEQPSMEEILASIRRIIAEDAATPPAAPAAPAEAAAE
                     EVLELTEMVKDDGTVVSLSAGAKAPAAPSPPDLKTAAGSMAQKKPASAEAPRVEPPPP
                     AAPLVEAPPPRVDASIELGTRHKQPEPKPAPPPIAAGDMSLELTEVEPEAPADTPPDA
                     AAAAEEHLISGATAATSMAALSRLADLGQHGIVSKEPLDESGRTLESLVRELLRPMLK
                     HWLDDHLPPMIERIVREEIQRMARDAKVR"
     gene            complement(2179..3546)
                     /locus_tag="VGP50_10530"
     CDS             complement(2179..3546)
                     /locus_tag="VGP50_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEV8027857.1"
                     /translation="MRPSLPVSCAIASLLAAAPLGLRAETLGEALVDAYANNPQILAE
                     RALLRATDENVPQALAGWRPTVQFTGSGGREHQDNNPPSVISPGGTNLTPLAADVNVT
                     QPIFNGGRTVALTAQAEKTVEAERARYVATEEATFLSVAQAYLDVVRDQATLELSINN
                     EQVLRRQLEATSDQFRVGAVTRTDVAQAEARLAGATASRLQAEGNLQVSRANYERAVG
                     HPPEALAQPSLRPTLPVTRDDAVAMAAGKNPNVIAALFTEDAARDFVDATRAQLLPSL
                     ALVGDANRSRETSFRGLETTTYSVIARVTVPLYEGGAIYSQTRQATETVGQRLGQTDD
                     ARRAAIQGATQAFEQVTSGRAQVVSLQSTIRAAEIALDGVRQEATVGSRTVLDVLNAE
                     QELFTDQVQLVTAQHDVAVAEFTLASQIGQLTAADLKLPVALYDVDRHYRSVREKWIG
                     FGAKD"
     gene            complement(3573..4229)
                     /locus_tag="VGP50_10535"
     CDS             complement(3573..4229)
                     /locus_tag="VGP50_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEV8027858.1"
                     /translation="MIDYAAARLNMVESQLRTNTVTDRAVLDAFLAVPRERFVPPAVQ
                     RTAYVDERLPLGGRRWLMEPMVLARLIQLAAIGEDDIVLDVGCGSGYAAAVLARLARQ
                     VVAVDSEPLLVAQAVARLRELGAGNVLAVEAPMAEGYPARAPYDAILIEGGVTQLPEA
                     MSRQLAEGGRLVTLMGEGNGVGHAVIMSKSGGVLSRRPVFDAASPLLPGFERAPSFQF
                     "
     gene            4369..4442
                     /locus_tag="VGP50_10540"
     tRNA            4369..4442
                     /locus_tag="VGP50_10540"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4401..4403,aa:Cys,seq:gca)
BASE COUNT          692 a         1562 c         1657 g          788 t
ORIGIN      
        1 cgggcccata ccaggccggg atctgatgac cccaccagag ctggcgcgag atgttccagg
       61 gctggatgtt gcgcatccac tcgaagaagg tgttctccca gctcttcggc acgaacaccg
      121 tgcgcccggt ctcgaccgcg gcgatcgccg ccttggcgag cgtcgcggca tcgcaatacc
      181 actggtccga caggtagggc tcgagcacca ccttggagcg gtcgccatgg ggcaccatga
      241 gggtgtgcgg cacgatcttt tcgacgaggc cctgcgcttc gagatcggcg acgatcttct
      301 tgcgcgcatc gaagcgatcg aggccgcgat acgcctcggg cgtcgcctcg ttcagcttcg
      361 cgtccttgtc gaagatgttg atgagcgcga gattgtggcg gcggccgacc tggaaatcgt
      421 tgaagtcgtg cgccggcgtg atcttgaccg cgccgctgcc ggccgtcgga tcggcatagt
      481 cgtcgccgac gatctgaatg cgccgcccca ccagcggcag gatcgcatgc ttgccgatca
      541 gatcctggta gcgctcgtcc ttggggttca ccgcgacgcc gctgtcgccg agcatggtct
      601 cgggccgcgt cgtcgccacg gtgatgaagc gcccctcctc gccctcgacc ggatatttga
      661 agtaccagag cgagcctttg gtctcgtgga actcgacctc gagatcggag aggccggtat
      721 gcagctccgg atcccagttg acgaggcgct tgtcgcgata gatgagccct tccttgtgga
      781 gccgaacgaa gacgcggcgc acggcggcgg agaggccttc gtccatggtg aagcgctcgc
      841 ggctccaatc gggtgaggcg ccgagcgcgc ggagctggcg ggtgatggtg ccgccggatt
      901 cggctttcca ctgccagacg cgctcgatga aagcggcgcg gccgagcgtg tggcggctcg
      961 actgctcgag cgcgagttga cgctccacca tcatctgcgt ggcaatgccg gcatggtcgg
     1021 ttccgggctg ccacagcgcg tcgcgtccgc gcatgcgatg gtagcggatc aggatgtcct
     1081 gcagcgtgtg gttgagcgcg tgcccgatat gcaagctgcc cgtcacgttg ggcggcggca
     1141 tcatgatgca atagggctcg gcgggagagt cgggatgggc ggcgaacgcg cccgaggctt
     1201 cccaggcggc gtagatcacc gcctcgacat cgcccggccg ataggtcttg tccagcatcg
     1261 ccccgtcagc ggaccttggc gtcgcgcgcc atgcgctgga tctcttcgcg cacaatccgc
     1321 tcgatcatcg gcggcagatg gtcgtcgagc cagtgcttga gcatgggccg cagcaattcg
     1381 cgcacgaggc tttcgagcgt gcgcccggat tcgtcgagcg gctccttgct gacgatgccg
     1441 tgctgtccga gatcggcgag acgcgagagc gccgccatcg atgtggcagc ggtggcgccg
     1501 gagatgaggt gctcctccgc tgcggcggct gcgtcgggcg gcgtgtcggc cggcgcctcc
     1561 ggctccacct cggtcagctc gagcgacatg tccccggcag cgatgggcgg gggtgcgggc
     1621 ttgggctccg gctgcttgtg ccgcgttccc aactcgatcg acgcatcgac ccgcggcggc
     1681 ggggcttcga ccagcggcgc ggctggcggc ggcggctcga cccgcggcgc ttccgcgctc
     1741 gccggttttt tctgcgccat gctgccggcg gccgtcttca ggtccggcgg cgatggtgcc
     1801 gccggcgctt tcgctcccgc ggagaggctc accacggtgc cgtcgtcctt caccatctcg
     1861 gtgagctcga gcacttcttc cgccgccgct tcggccggcg ctgcgggtgc agccggaggc
     1921 gtcgccgcgt cctcggcgat gatgcgccgg atggaggcga gaatttcctc catcgatggc
     1981 tgttcgggct ggctctctgc ttgcggcatg acgcttcgac cggatccggc gtgaaaacgc
     2041 cggctacgtt aggcgaaaca acgccaaaac gccagcggcc ggcaaacaag agattgacca
     2101 atgaggccct gaggcggtca gagaacgacg gcgccgagcc tcaccgtctc actgcctcgc
     2161 tggtgaatct tgtttgtctt agtctttggc gccgaagccg atccacttct cgcggacgga
     2221 ccggtagtgg cggtcgacgt cgtagagcgc aaccggcaat ttaaggtcgg cggccgtgag
     2281 ctggccgatc tgcgacgcca gcgtgaactc ggcgacggcc acatcgtgct gcgccgtcac
     2341 cagctgcacc tggtcggtga acagctcctg ctcggcgttg agcacgtcga ggacggtgcg
     2401 cgagccgacc gttgcctcct ggcggacgcc gtcgagcgcg atctcggcgg cgcggatcgt
     2461 cgattgcagc gacacgacct gagcgcggcc cgacgtcacc tgctcgaagg cctgggtcgc
     2521 gccctggatc gccgcgcgcc gcgcgtcatc ggtctggccc aggcgctgac ccacggtctc
     2581 ggtcgcctgg cgcgtctgcg agtagattgc accgccctcg taaagcggca ccgtgacgcg
     2641 cgcgatcacc gagtaggtgg tggtctcgag gccgcggaaa ctcgtctcgc ggctgcggtt
     2701 ggcgtcgccg acgagagcga ggctcggcag cagctgggcg cgcgtggcat cgacgaagtc
     2761 gcgcgcggcg tcctcggtga aaagcgcggc gatgacgttg ggattcttgc ctgcggccat
     2821 cgccacggcg tcatcgcgcg tcacgggcag cgtcggacgc aggctcggct gggcgagcgc
     2881 ctcgggcgga tggccgacgg cgcgctcgta attggcgcgg ctcacctgca gattgccctc
     2941 ggcctgaagc cgggacgcgg tcgctccggc gaggcgcgct tccgcctgcg ccacgtcggt
     3001 gcgcgtcacc gcgccgaccc ggaactggtc gctggtcgcc tcgagctgcc ggcgcagcac
     3061 ctgttcattg ttgatcgaga gctcgagcgt cgcctggtcg cgcaccacgt cgagataggc
     3121 ctgggcgacg ctcaggaagg tcgcctcctc ggtcgcgaca tagcgcgccc gctcggcctc
     3181 gacggttttt tccgcctggg cggtcagcgc cacggtgcgg ccgccgttga agatcggctg
     3241 cgtcacgttg acgtcggcgg caagcggcgt gaggttcgtc ccgcccggcg agatcaccga
     3301 aggcgggttg ttgtcctggt gctcacggcc gccggagccg gtgaactgca cggtggggcg
     3361 ccagccggcg agggcctgcg gcacgttctc gtcggtggcg cggagcagcg cacgctcggc
     3421 gaggatctgc ggattgttgg cataggcatc gaccagcgcc tcgcccagcg tctctgcccg
     3481 gagcccgagc ggcgccgcgg cgaggagaga agcgatggcg caggagacgg gcaatgaggg
     3541 gcgcatgggt gcctgatgca ggggccgggg cgctagaatt ggaagctcgg cgcgcgctcg
     3601 aagccgggga gcagcggcga tgcggcatcg aagaccgggc gccgcgacaa cacgccgccg
     3661 ctcttcgaca tgatcactgc gtggccgacg ccgttcccct cgcccatcag ggtcacaagg
     3721 cgcccgccct cggcgagctg gcggctcatc gcctccggga gctgcgtcac gccgccttcg
     3781 atgaggatcg cgtcataagg cgcccgcgcc ggatatccct cggccatcgg cgcttcgacg
     3841 gcgagcacat tgccggcgcc cagctcgcgc agccgtgcca ccgcctgcgc gaccagaagc
     3901 ggctcggaat cgacggcgac gacctggcgc gccagccgcg ccagcaccgc cgcggcatag
     3961 ccgctgccgc agccgacatc gaggacgatg tcgtcctcgc cgatggcggc gagctggatg
     4021 agccgcgcca gcaccatcgg ctccatcagc cagcggcggc cgccgagcgg caggcgctcg
     4081 tcgacatagg cggtgcgctg caccgccggc ggcacgaagc gctcgcgcgg cacggcgaga
     4141 aacgcgtcga gcaccgcccg gtcggtcacc gtgttggtgc ggagctggct ctcgaccatg
     4201 ttgaggcgcg ccgcggcgta gtcgatcatg ggcactcagg tgcaaagagc gggccaagga
     4261 cgcgccgttt atatcgtcgc gcggcggcga agacaatcgg gaggctcgaa gcgcacgccc
     4321 cggcaacctt gaccctccgc gcaggccgcg cctataaggc gcgcgcgcgg ccggatggca
     4381 gagtggctat gcagaggact gcaaatcctc gtacagcggt tcgattccgc ttccggcctc
     4441 cacccaataa acccttgata aataggcatt tttggccgcc cgcgagcggc cttcttgtgt
     4501 ccgaatcgtg ttgcaacacg ctgccaacag aaacggcgga tttcctggcg ttaccacgca
     4561 ttccatttgt tccatgcaac acgcgtgtaa cacggacgag caggttttgt tcccgcggga
     4621 ctgcgggtgc ttatgtcgga cccgtgccgc gccgttatct aacctgcacg gggccggctc
     4681 gcggtctctg cttgtcgtg
//
LOCUS       DASYNS010000337         5876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_863297, whole genome shotgun sequence.
ACCESSION   DASYNS010000337 DASYNS010000000
VERSION     DASYNS010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863297"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..694)
                     /locus_tag="VGP50_10545"
     CDS             complement(272..694)
                     /locus_tag="VGP50_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027859.1"
                     /translation="MSGAIARRMLLLGALLIAQGSLFGAAAAGDDGMPRAKRVPPPNV
                     APITIDDVEFAAIPFGKARGLGQNGGYVAARDPATGKEIWMLKVYDVVYDPKLEGDVQ
                     DVFIRSLQKSASGHELVVTDERGRRYLVDPRTRIVRQQ"
     gene            complement(691..1026)
                     /locus_tag="VGP50_10550"
     CDS             complement(691..1026)
                     /locus_tag="VGP50_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027860.1"
                     /translation="MSGAPKAKRVAPAKVLPVTVGGLRFEAIHWGKARDLGQNGGYIA
                     AIDPATDKELWTLKVYDVVYDPKLEGDVQDVFITTISKTLFGQKLNIRDEKGRGYIVD
                     PATRAVRTR"
     gene            complement(1023..2108)
                     /locus_tag="VGP50_10555"
     CDS             complement(1023..2108)
                     /locus_tag="VGP50_10555"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF025874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M35 family metallo-endopeptidase"
                     /protein_id="HEV8027861.1"
                     /translation="MSSTFTPVYQKAMAVLTGQKFAEPDWQKFLAETCGAIRLLGPAG
                     FDPAYAGGLDSIRTKIKGDSKHSNSVAIFFLGGGPGEVIYNAAQNKSSGGSWNDRAAT
                     LKMLRHVYRARKSGGQDVWVYAPPKDHGRQVFDELAGADAAQAKKKLGRDSEIFDEAE
                     RGLMCDALQLARKIAMDCSIKVTAKSAGAKTAVRRWFMDEKCTKTELDNALAKLATGM
                     NKIAAACNSPSLVFTDYLDWRKARNSYFGGAIPGGEGGGFPVIYLEGAFTRLKGNSGK
                     LWLCAETIIHELSHHEVGTDDHFYDNDGLKPNSGTFPYSKAIDNADSWGYFAVDLAGY
                     LTSTDRLGTGVDDGPEISEDEPKRKVA"
     gene            2371..3363
                     /gene="nadA"
                     /locus_tag="VGP50_10560"
     CDS             2371..3363
                     /gene="nadA"
                     /locus_tag="VGP50_10560"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEV8027862.1"
                     /translation="MQAVSLAQDVIARTAPLYERVRRVIPPIEWPVFADDIDAILRLK
                     RAKNAVILAHNYQTPEIFHCVADIVGDSLALAREAMTAKADVIVLAGVHFMAETAKLL
                     NPEKRVLIPDLEAGCSLAASITAADVKLLRERYPGAPVVTYVNTSAAVKAESDICCTS
                     GNAKKVVEALGVPRVIMIPDEYLAQNIARETGVEIVTWAGHCEVHERFTAAEIRELRA
                     RHPGVIVLAHPECPPDVVAEADFAGSTAAMSDYVGKRKPARVVLVTECSMSDNLAVQH
                     PAIEFVRPCNLCPHMKRITLPKIRRALETMTHEVTIDPAVAVRARQAVERMLAI"
     gene            3374..4870
                     /locus_tag="VGP50_10565"
     CDS             3374..4870
                     /locus_tag="VGP50_10565"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF005701.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEV8027863.1"
                     /translation="MDSNDRPVVVGAGLAGLMTALHLAPEPVVVLARTPLGSGIASAF
                     AQGGIAAALGDGDDPFLHAADTVAAGDGLADPDVVERITAAAPAAIEELVRRGVAFDR
                     GADGRFLLGLEAAHCRRRIVHAGGDRTGGEIMRALVEAVRRTPSITVLEGVEARRLLV
                     AEGGIAGVLAAGASGAQTLAASRVVIATGGIGGLYRHTTNPLGALGQGVALAARAGAL
                     LADMEFVQFHPTALDVGLDPMPLVSEAVRGEGAVLVDETGARFTDELQPRDQVSRAVW
                     RHQAEGHRVFLDARSALGGRFAERFPAIAARCRAAGVDPAREPIPVRPAAHYHMGGIA
                     VDAAGRSSVAGLWACGEAAATGLHGANRLASNSLLEAAACARWVAGSVAGAPERRLPL
                     PSLPALPPAAAAGAVRQIMSAGLGVLRDRAGLSKAIAALRPLAFAGGAAADAAAVGLV
                     IAVAALAREESRGGHWRSDFPFRASGAARRSKLRLSDALRVLAEVAGG"
     gene            4875..5726
                     /gene="nadC"
                     /locus_tag="VGP50_10570"
     CDS             4875..5726
                     /gene="nadC"
                     /locus_tag="VGP50_10570"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003617937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV8027864.1"
                     /translation="MTVPPLPTIMIEPLVRAALLEDLGRAGDITTDAIVPPEARAETA
                     LTARQKGVLAGIDLAVTAFRLIDPAIGMRLECRDGDRLSPGDVIAIVEGPARGILTAE
                     RVALNFLCHLSGVASATAGIVDTIKGHRARVVCTRKTMPGLRAVQKYAVRIGGGSNHR
                     FGLDDAVLIKDNHIAVAGGLAPAVERARAAIGHLVKVEVEVDTFVQLEEALRLSVDAV
                     LLDNMSLEELRRAVALVDRRAITEASGRITPETAPAIAATGVDLISVGWLTHSAAVLD
                     IGLDYRG"
BASE COUNT          894 a         2006 c         1996 g          980 t
ORIGIN      
        1 gggactggtc gaagagaccg gcgccatctc cacgggcacc ggcggccgtc ccgccaagct
       61 cttccgcttc cgccgcgaag tgctgcgcga gcgcgccgtt tccggctcga agctgccgct
      121 gtcgcgctcg gcctaggact ggtgacggca cttcccagaa gaggaacgcc gagggcgcgg
      181 aggtctcgca aaggacgcgc agagaaggat cgttactctg cgtcctccgc gcatcctcgg
      241 cgtcctctgc gttcctctaa tagttgcgcc gctattgctg ccggacgatg cgcgtccttg
      301 gatcgacgag gtagcgccgg ccgcgctcgt cggtcacgac gagctcgtgg cccgacgcgc
      361 tcttctggag agaccggatg aacacgtcct gcacgtcgcc ctccagtttc ggatcataga
      421 cgacgtcata gaccttgagc atccagatct ccttgccggt cgccggatcg cgcgcggcga
      481 catagccgcc attctggccg agcccgcgcg ccttcccgaa ggggatcgcg gcgaattcga
      541 cgtcgtcgat ggtgatggga gcgacgttgg gcggcggcac gcgtttcgcg cgcggcatcc
      601 cgtcatcacc ggccgcagcg gcgccgaaga gcgatccttg cgcgatgaga agcgcgccga
      661 gcagcagcat tcgccgggca atggcaccgc tcaacgagtc ctcaccgcgc gtgtcgcggg
      721 atcgacgatg taaccacgtc ctttctcatc cctgatgttc agtttctggc caaaaagcgt
      781 tttggatatg gtggtgatga acacatcctg cacatcgccc tcgagcttcg gatcgtagac
      841 gacgtcatac accttcaacg tccaaagctc cttgtcggtc gcaggatcga tcgccgcgat
      901 atagccgccg ttctggccga ggtcgcgcgc cttgccccaa tggatcgcct cgaagcgcaa
      961 gccgccgacg gtgaccggca agaccttcgc cggcgccacg cgctttgctt tgggcgcgcc
     1021 gctcatgcga ccttccgctt cggctcgtct tccgagatct cgggcccgtc atcgaccccg
     1081 gtgccgaggc ggtccgtcga ggtgagatat cccgcgagat cgacggcgaa atagccccag
     1141 ctgtcggcgt tgtcgatcgc cttgctgtaa gggaaggtgc cgctgttggg cttgagcccg
     1201 tcattgtcgt agaaatgatc gtccgtgccg acctcgtgat gcgacagctc gtgaatgatg
     1261 gtctcggcac agagccaaag cttgccgctg ttgcccttga gacgcgtaaa ggcgccttcg
     1321 agatagatca ccgggaagcc gccgccctca ccgcccggga tagcgccgcc gaaataagaa
     1381 ttgcgcgcct tgcgccagtc gaggtagtcg gtgaagacca gcgagggcga gttgcaggcg
     1441 gccgcgatct tgttcatgcc cgtcgcgagc ttcgcgagcg cgttgtcgag ctcggtcttg
     1501 gtgcatttct catccatgaa ccagcgccgc accgcagtct tggcaccggc gctttttgcc
     1561 gtgaccttga tcgagcaatc cattgcgatc ttgcgcgcaa gctgcagcgc atcgcacatc
     1621 agcccgcgct cggcctcgtc gaagatctcc gagtcccggc cgagcttctt cttggcttgc
     1681 gcggcatcgg cgccggcgag ctcgtcaaag acttgcctgc cgtggtcctt cggcggcgca
     1741 tagacccaga cgtcctggcc tccgctcttg cgcgcgcgat agacgtggcg gagcatcttc
     1801 agcgtcgcgg cgcgatcgtt ccatgagccg ccggaactct tgttctgcgc ggcgttgtag
     1861 atcacctcgc ccggcccgcc gccgagaaag aagatcgcca ccgaattgct gtgcttgctg
     1921 tcgcctttga tcttggtgcg gatgctgtcg agcccgcccg cataggccgg gtcgaagcca
     1981 gcggggccga gcagcctgat ggcgccgcag gtctctgcca ggaatttctg ccagtcgggc
     2041 tcggcgaatt tctggccggt gaggaccgcc atcgccttct gatagaccgg cgtgaatgtg
     2101 ctgctcatgt caaagccccc cgttcttccc cgccccgcgc ggcggcagcg ccgacccgct
     2161 gaaggaaagc ttagcagggg ccgcagacgc gtcgaggaga aatcccgttt cagcgatact
     2221 tttacgcgag cgtgcgcgcc gcgcacccct gccatgccgc gcgcgaatcg caagaatcgt
     2281 acgacgaaca cccttgaccc ggcttatgct catccatagc ataatgggga ggaaatgctc
     2341 aacttgagta taaaatgagg taggtgggtc atgcaggctg tctctctcgc ccaggacgtc
     2401 atcgcccgga ccgcgccgct ctacgagcgc gtccgccggg tgatcccgcc gatcgaatgg
     2461 ccggtcttcg ccgacgacat cgacgccatc ctgcgcctga agcgcgcgaa gaacgccgtg
     2521 atcctcgcgc acaactatca gacgccggag atcttccact gcgtcgccga catcgtcggc
     2581 gacagcctgg cgctcgcgcg cgaggcgatg acggcaaagg ccgatgtcat cgttctggcc
     2641 ggtgtccatt tcatggcgga gacggcaaag ctgctcaatc cggaaaagcg cgtgctgatc
     2701 cccgatctcg aggcgggctg ctcgctcgct gcgtcgatca ctgccgccga cgtgaagctg
     2761 ttgcgtgagc gctatccggg cgcgccggtc gtgacttacg tcaacacttc ggccgcggtc
     2821 aaggccgagt ccgacatctg ctgcacctcg ggcaacgcca agaaggtggt cgaggcgctg
     2881 ggcgtaccgc gcgtgatcat gatcccggac gaatatctgg cgcagaacat cgcgcgcgag
     2941 acgggcgtcg agatcgtcac ctgggccggt cattgcgaag tgcatgagcg cttcaccgcc
     3001 gccgagatcc gcgagctgcg cgcgcgccat ccgggcgtca tcgtgctcgc ccatcccgaa
     3061 tgcccgcccg acgtggtggc ggaagcggac ttcgccggct cgaccgcggc aatgagcgac
     3121 tatgtcggca agcgcaagcc ggcgcgcgtc gtgctggtga cggagtgctc gatgagcgac
     3181 aacctcgcgg tccagcatcc cgcgatcgaa ttcgtgcgtc cgtgcaatct ctgcccgcat
     3241 atgaagcgga tcacgctgcc gaagatccgc cgcgcgctcg agacgatgac gcatgaagtg
     3301 acgatcgacc cggccgtcgc ggtgcgcgcg cggcaggcgg tcgagcgcat gctggcgatc
     3361 taggagcaag cccgtggaca gcaacgaccg tcccgtcgtc gtcggcgccg gcctcgccgg
     3421 gctgatgacg gcattgcatc tggcgcctga gccggtggtt gtgctggcgc ggacgccgct
     3481 tggctccggc atcgccagcg ccttcgcgca aggcgggatc gccgcggcgc tcggcgatgg
     3541 cgacgatccc ttcctccatg cggccgatac cgtggcggca ggcgacggcc tcgcggatcc
     3601 cgacgtcgtc gagcgcatca cggcggccgc gccggccgcg atcgaggaat tggtgcggcg
     3661 cggcgtggcc ttcgaccgcg gcgcggacgg ccgctttctg ctgggactcg aagccgcgca
     3721 ttgccggcgc cgcatcgtcc atgccggcgg cgaccgcacc ggcggcgaga tcatgcgcgc
     3781 gctggtcgaa gcggtgcggc gcacgccctc gatcaccgtt ctcgaaggcg tcgaagcgcg
     3841 ccggctcctg gtcgcggagg gcggcattgc cggcgtgctc gccgccggtg cttcgggcgc
     3901 ccagactctg gcggcaagcc gcgtcgtgat cgccacgggc ggtatcggcg gactctatcg
     3961 ccacaccacc aacccgctgg gcgcgctggg ccagggcgtg gcgctggcgg cgcgcgccgg
     4021 ggcactcctc gccgacatgg agttcgtgca gttccacccg accgctctcg atgtcggcct
     4081 cgaccccatg ccgctcgtga gcgaagcggt gcgcggcgaa ggcgcggtgc tcgtcgacga
     4141 gacgggcgcg cgcttcaccg acgagctcca gccgcgcgat caagtgtcgc gcgcggtgtg
     4201 gcggcaccag gccgaggggc accgcgtctt cctcgatgcg cggagcgcgc tgggcgggcg
     4261 cttcgccgag cgctttccgg caatcgccgc gcgctgccgc gcggccggcg tcgatccggc
     4321 gcgcgagccg atcccggtgc ggccggcagc gcattaccac atgggcggca tcgccgtcga
     4381 cgcagccggg cggagcagcg tggccggact ctgggcctgc ggcgaagcgg cggcgacggg
     4441 cctccacggc gccaatcgcc tcgccagcaa ttccctgctc gaggccgccg cttgcgcgcg
     4501 ctgggtcgcc gggagcgtcg ccggagcgcc ggagcggcgc ctgcccttgc cgtcgctgcc
     4561 ggccctgccg ccggccgcgg cagccggagc ggtgcggcag atcatgtcag ccggtctcgg
     4621 cgtgctccgg gaccgcgccg gcttaagcaa ggcgatcgcg gcgctgcggc cgctcgcctt
     4681 cgctggcggc gctgcggcgg atgcggcggc ggtgggcctc gtcatcgcgg tggcggcgct
     4741 tgcgcgcgaa gagagccgcg gcgggcattg gcgcagcgat tttccgttcc gcgcgagcgg
     4801 agcggcgcgc cgctcgaagc tgcggctttc cgatgccctg cgcgttcttg ccgaggtcgc
     4861 gggaggatga agcgatgacc gtgccgccgc tgccgaccat catgatcgag ccgctggtgc
     4921 gcgccgcgct gctggaggat ctcggccgtg ccggcgacat cacgaccgac gccattgtgc
     4981 cgccggaggc gcgcgccgag acggcgctga cggcgcggca gaagggcgtg ctggccggga
     5041 tcgatctggc ggtgacggcg ttccgcctga tcgatcccgc gatcggtatg cggctcgaat
     5101 gccgcgacgg cgaccgcctg tcgccgggcg acgtcatcgc gatcgtcgag gggccggcgc
     5161 gcggcatcct caccgccgag cgcgtcgcgc tcaattttct ctgccatctc tcgggcgtcg
     5221 catcggccac cgccggcatc gtcgacacga tcaaaggcca tcgcgcgcgc gtcgtctgca
     5281 cacggaagac catgccgggc ttgcgcgccg tgcagaaata cgcggtgcga atcggcggcg
     5341 gcagcaatca ccgcttcggc ctcgatgacg ccgtgctcat caaggacaat cacatcgccg
     5401 tggccggcgg cctcgcgccg gcggtcgagc gcgcccgcgc cgccatcggc catctcgtca
     5461 aggtcgaggt cgaggtcgac acgttcgtgc agctcgaaga agcgctgagg ctcagcgtcg
     5521 atgcggtgct gctcgacaat atgagtctcg aggagctgcg ccgcgcggtg gcgctggtcg
     5581 accgccgcgc catcaccgag gcctcgggac gcatcacgcc ggagacggcg ccggcgatcg
     5641 ccgcgacggg cgtcgatctc atctccgtcg gctggctcac gcacagcgcc gcggttctcg
     5701 acatcggtct cgattatcgc gggtgaggcc ccctcaccct cccgccctcg agagaaaatc
     5761 ccgagcaggt ggaatcggga tgcgctctaa gcagcgttgc ttagactaag atacgaatac
     5821 atcaatcccc tctcccatcg gggagagggt cagggtgagg gggccgcgcc acgatg
//
LOCUS       DASYNS010000338         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5231258, whole genome shotgun sequence.
ACCESSION   DASYNS010000338 DASYNS010000000
VERSION     DASYNS010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5231258"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VGP50_10575"
     CDS             <1..551
                     /locus_tag="VGP50_10575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEV8027865.1"
                     /translation="KNLELVRREALTDALTGIPNRKFFDTRLRAAVLEANNTGEPLSL
                     VITDIDHFKHFNDTYGHQMGDQVLRLVARTLTDSVKGRDTPARYGGEEFALILPQTRL
                     ADALVLAEQIRRTIMRRHIVRRDTGDDFGTITLSMGVAAYRPTEHLTDTLGRADAGLY
                     QAKRSGRNRVVSEEALEKHAAE"
     gene            744..965
                     /locus_tag="VGP50_10580"
     CDS             744..965
                     /locus_tag="VGP50_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027866.1"
                     /translation="MGRTAVAESHADWGPGGAWSIGAALALFILPWSAMICSIRLWRR
                     NLTPGWMSHVLNNIVAYLVLPSLHLGQHP"
     gene            962..1522
                     /locus_tag="VGP50_10585"
     CDS             962..1522
                     /locus_tag="VGP50_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027867.1"
                     /translation="MTIDPREAASSLDDIAGIERRMREALFYGGSSNILIAWGIICAI
                     GHCITQFSPRNAGLAWLLLDAVGVVAAVVISVRRARQRRRDWNWRFAAGITVTVAFGL
                     LWQWLLGDGQWREVSAFWATLIMFTYILAGLWVGRFFVVCGIVVTALTLVGYFWSGAW
                     FELWMAAVFGGSLVAGGLWLRRVGAR"
     gene            1522..1830
                     /locus_tag="VGP50_10590"
     CDS             1522..1830
                     /locus_tag="VGP50_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV8027868.1"
                     /translation="MAAPNDVIHQSMRLKIMAALNVLNADAQLDFGRLRALVGATDGN
                     LGAHLETLEKAGYVAIEKLFVGKKPQTRVRATPSGRHAFGEHVAYLRHILNGTGERPS
                     "
     gene            1827..>2260
                     /locus_tag="VGP50_10595"
     CDS             1827..>2260
                     /locus_tag="VGP50_10595"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV8027869.1"
                     /translation="MSRKKAIVLLVAAIVAAPVLTLGGMLAFGTAAPPPSLASVYDNV
                     RRIDQSGLPPLARFTARDGSALAYRTWPGGGERVAVLVHGSSGRSSNMNPLAKMLVAD
                     GATVYAMDMRGHGESGRRGDIDYIGQMDDDMADFMALIKPHHP"
BASE COUNT          374 a          778 c          731 g          377 t
ORIGIN      
        1 gcaagaacct cgagctggtg cggcgcgagg cgctgaccga cgcgctcacc ggcattccca
       61 atcgcaaatt cttcgacacc cggctgcgcg ccgccgtgct cgaagccaac aacaccggcg
      121 agccgctctc gctggtcatc accgacatcg atcatttcaa gcatttcaac gacacctacg
      181 gccatcagat gggcgatcag gtgctgcgcc tcgttgcgcg gacgctgacc gacagcgtca
      241 agggccgcga cacgccggcg cgctacggcg gcgaggaatt cgctctgatc ctgccgcaga
      301 cccggctcgc ggatgcgctg gtgctcgccg agcagatccg ccgcaccatc atgcgccgcc
      361 acatcgtgcg ccgcgacacc ggcgacgatt ttggcaccat cacgctctcg atgggcgtcg
      421 ccgcctatcg tcccaccgag catctgacgg acacgctcgg ccgcgccgat gccgggctct
      481 atcaggcgaa gcgctcgggc cgcaaccgcg tcgtctccga agaagccctc gagaagcacg
      541 cggcggagtg aggcgagcgc gcaaagcgcg cccgcctcac tccgtccgcg gcggcggcag
      601 cggtttgcgt cagcaaaccg cgagagccgc accaagcaga accacctcca ttacgccgcg
      661 cgcgcctcgc ccgctggcaa cgccttgaca aaccagagca ctctgtgata cagaatattc
      721 tggatggtgc gatgatgagc gaaatggggc ggacggccgt cgcggaaagc cacgcggatt
      781 ggggcccggg cggcgcttgg agcatcggcg ccgccctggc gctcttcatc ctgccgtggt
      841 cggcgatgat ctgcagcata cggctgtggc gccgaaacct gacccccggc tggatgagcc
      901 acgtcctcaa caacatcgtc gcctatctcg tccttccctc gctgcacctc ggacaacacc
      961 catgaccatc gatccgcgcg aagccgcaag ctcactcgac gacatcgccg gcatcgagcg
     1021 gcgcatgcgc gaggcgctgt tctatggcgg cagcagcaac attctcatcg cgtggggcat
     1081 catctgcgcg atcgggcact gcatcactca gttcagtccg cgcaacgccg gtctcgcctg
     1141 gctgctgctt gatgccgtcg gtgtcgtggc cgccgtcgtc atcagcgtcc ggcgcgcccg
     1201 ccagcggcgg cgcgactgga actggcgctt cgccgccggc atcaccgtca ccgtcgcttt
     1261 cgggctgctc tggcaatggc tgctcgggga cgggcaatgg cgcgaggtca gcgccttctg
     1321 ggcgacgctg atcatgttca cttacatcct cgcaggattg tgggtcggcc gcttcttcgt
     1381 cgtctgcggc atcgtcgtca cggcgctgac gctggtcgga tatttttgga gcggcgcctg
     1441 gttcgagctg tggatggccg cggtcttcgg cggctcgctg gtggcgggcg gcctgtggct
     1501 gcgccgggtc ggagcgcgct gatggcggcg cccaacgacg tcattcatca gtcgatgcgg
     1561 ctcaagatca tggccgcgct caatgtgctc aatgccgacg cgcagctcga tttcggacgg
     1621 ctcagagcgc tggtcggcgc gaccgatggc aatctcggcg cccatctcga gacgctggag
     1681 aaagccggct atgtcgccat cgaaaagctg ttcgtcggca agaagccgca aacacgggtg
     1741 agggcgaccc catcgggccg gcacgccttc ggcgagcatg tcgcttacct ccgccacatc
     1801 ctcaacggaa cgggagagag gccatcatga gcaggaaaaa ggcgatcgtc ttgctggtcg
     1861 cggcgattgt cgccgcgccc gttctgacgc tcggcggcat gctggctttc ggcacggccg
     1921 cgccgccgcc gtccttggca tcggtctatg acaatgtgcg ccgcatcgat cagagcggtc
     1981 tgccgccgct cgcgcgcttc accgcgcgcg acggcagcgc gctcgcctat cgcacctggc
     2041 cgggcggggg cgaacgcgtc gccgtcctcg tccatggctc gagcggcagg agctccaaca
     2101 tgaacccgct ggcgaagatg ctggtcgccg acggcgccac cgtctatgcg atggacatgc
     2161 gcggccatgg cgagagcggg cggcgcggcg acatcgacta tatcggccag atggatgacg
     2221 acatggccga cttcatggcg ctgatcaagc cgcatcatcc
//
LOCUS       DASYNS010000339         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4922354, whole genome shotgun sequence.
ACCESSION   DASYNS010000339 DASYNS010000000
VERSION     DASYNS010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4922354"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="VGP50_10600"
     CDS             complement(<1..983)
                     /locus_tag="VGP50_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEV8027870.1"
                     /translation="MALTRETADGSDKLSPVPADSATAPQTERPKLRSFRPLLHLLQF
                     LRPYRRHLAGATIALVISAAAVLMVGQGLRHVVDQGLGAGNPDLLDRTVAALGLIVVV
                     LAVSTYSRFSLVSWIGERVVADLRIAVFERIVSLSPAYFETTQTGEILSRLTTDTTLL
                     QTVIGSSVSMALRNALILIGGLIMLVVTSVKLAGIVLVIVPLVVVPILVFGRRVRTLS
                     RRSQDRIADVGAYAEEMINAIRTVQAFTHEPIDRARFATRVEESFTTAVSRIRVRAIL
                     TAIVILLAFGAIAVVLWIGGYDVIAGRMSGGELSAFMFYAVLTAFAVGTIS"
     gene            1259..1642
                     /locus_tag="VGP50_10605"
     CDS             1259..1642
                     /locus_tag="VGP50_10605"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV8027871.1"
                     /translation="MVGRGIVFSGEIKSCDCLVVEGTVKANIADCHDINIAEGGLFTG
                     SAAVDRAEIRGRFEGTLAVAEHLLIHSTGRVAAEARYNQIEIERGGEISGTIQAQAQA
                     QGTTPVVKPAPEATQPAAVAMPRRA"
     gene            complement(1690..2031)
                     /locus_tag="VGP50_10610"
     CDS             complement(1690..2031)
                     /locus_tag="VGP50_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027872.1"
                     /translation="MAAPIDCARIRPKIKGMDLETFKASLTGEKPPAGIGRLTSGLWW
                     DAKGNWDKAHDCAQAQDDRDGAWLHAYLHGKEGDAENAGYWYRRAGKPVATEPLALEW
                     ETIARALLGAD"
     gene            2121..2471
                     /locus_tag="VGP50_10615"
     CDS             2121..2471
                     /locus_tag="VGP50_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027873.1"
                     /translation="MYCLYASDYGPADPAFDPFVDNGHLLEGFESHVYELATARNFAQ
                     LQAMERQLQRGQEIAWCDQGYGWAYRQPVNDFFIVAMIAVVLPYVVLRLVPRIGAAFT
                     AVAATRFNRPGDAS"
     gene            2468..>3340
                     /gene="secF"
                     /locus_tag="VGP50_10620"
     CDS             2468..>3340
                     /gene="secF"
                     /locus_tag="VGP50_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEV8027874.1"
                     /translation="MMRPLRLVPDGTAIKFMRGRFAGLITSAVLSLASVVLFFYPGLN
                     YGVDFKGGAIVEIRLPAPADLAAMRAAFDGLGFGEVQLQQFGAPEDVLVRLGQQAQGE
                     TARKSIQDMLALNFPGSEIRRLEIVGARVSDELFRNGMLATALALAAVLAYIWVRFDW
                     QFGVGVVGTLLLDVTKTVGFLALARLQFDLNAVAAILLLIGYSVTDKVVVYDRIRENL
                     RKFKTMPLRELIDLSINQTLGRTVATSLTVLLSILPLAVIGGEAIRGFALIIIFGIVV
                     GTSSSIFIAAPILLF"
BASE COUNT          559 a         1140 c         1050 g          591 t
ORIGIN      
        1 tccgagatgg tgccgaccgc aaaggccgtg agcacggcat agaacatgaa ggccgagagc
       61 tcgccgccgc tcatccgtcc ggcgatcacg tcatagccgc cgatccagag caccaccgcg
      121 atggcgccga aggcgaggag aatgacgatc gccgtcagga tggcgcgcac gcggatgcgt
      181 gacaccgccg tcgtgaagga ctcttcaacg cgtgtggcaa aacgcgcgcg gtcgatcggc
      241 tcatgcgtga aggcctggac ggtgcggatg gcgttgatca tctcctcggc ataggcgccg
      301 acatcggcga tgcggtcctg ggagcggcgc gagagggtgc gcacgcgccg cccgaaaacg
      361 aggatcggca ccaccaccag tggcacgatg acgagcacga tgccggcgag cttgacgctc
      421 gtcactacca gcatgatgag gccgccgatg aggatcagcg cattgcgcag cgccatcgac
      481 acgctggagc cgatgacagt ctgcagcagc gtcgtatcgg tggtgaggcg cgacaggatc
      541 tcgccggtct gggtggtctc gaaataggcc gggctcagcg acacgatgcg ctcgaacacg
      601 gcgattctga ggtcggcgac gacgcgctcg ccgatccagg agacgagcga gaagcggcta
      661 taggtggaga cggcaagcac gaccacgatc aggccgagcg cggccaccgt ccggtcgagg
      721 agatcggggt tgccggcacc caacccctgg tcgacgacat ggcgcaagcc ctgtccgacc
      781 atcagcacgg cggccgcgga aatgacgagc gcgatcgtgg cgccggcaag gtgccgccgg
      841 tagggcctga ggaactgaag caggtgcagc agcggccgga agctgcgcag cttcggccgc
      901 tcggtttgcg gcgccgtcgc gctatcggcc ggcactgggg acaatttgtc gctgccgtcg
      961 gcggtctctc gtgtcagcgc catggcggca gggcctcgat gaagtggaat ctgggcctcc
     1021 gatatgggct ttctgctggt cgcgcacaag gcgcgcccct ttcccgccac aatcccatct
     1081 caaatttgcc tccgacgcgc gggttctcag agagtacact cgcgccgatc tttctctagc
     1141 tcgcccggac gggagtggtg tgacgatgag tagttggaaa cctgcaagca cgatggcgca
     1201 caatcctgtg ccgcctcaaa agcctgaatc ggcgtctcag accgcgacgc gcaagctggt
     1261 ggtaggaagg ggcatcgtct tctcgggcga gatcaaatcc tgcgactgtc tcgtggtcga
     1321 gggcacggtc aaggccaaca tcgccgattg ccacgacatc aacatcgccg aaggcggcct
     1381 cttcaccggc tcggccgcgg tcgaccgcgc cgagattcgc ggccgtttcg agggcacgct
     1441 cgccgtcgcc gagcatctcc tcattcattc gaccggccga gtcgccgccg aggcccgcta
     1501 taaccagatc gagatcgagc gcggcggcga gatctccggc acaatacagg cgcaggcgca
     1561 ggcgcaaggc accacacccg tggtgaagcc cgcgcccgaa gcgacgcagc ctgctgccgt
     1621 cgctatgccg cgccgcgctt aatgttttgt taaccctctc ccgcattgcg ggagagggca
     1681 aagtttcggc taatccgcgc ccagcaacgc gcgcgcgatc gtttcccact ccagggcgag
     1741 cggctccgtc gccaccggtt tgccggcgcg gcggtaccag tatccggcat tctccgcgtc
     1801 gccttccttg ccatgaagat aggcgtgaag ccaggcgccg tcgcggtcgt cctgcgcttg
     1861 cgcacagtcg tgcgccttgt cccagttgcc cttcgcgtcc caccataacc cggaggtgag
     1921 gcggccgatt ccggcgggcg gcttttcgcc tgtgagcgag gctttgaagg tttcgagatc
     1981 catgcccttg atcttggggc gaatgcgggc gcaatcaata ggcgcggcca cggcggctct
     2041 cgactttacg acttccttac accgaaggcc ttcggcccat atcgaaactt tacgcgccgc
     2101 gaccctagtc tcagcggccc atgtattgcc tctatgccag cgactacgga ccggccgatc
     2161 ccgccttcga ccctttcgtc gataacgggc atcttctcga gggattcgag tcccatgtct
     2221 atgagctcgc gacggcgcga aatttcgccc agctccaggc gatggagcgc cagctccagc
     2281 gcggccagga gattgcctgg tgcgatcagg gctatggctg ggcctatcgg cagccggtca
     2341 acgacttctt catcgtcgcc atgatcgccg tggtcctgcc ttacgtcgtc ctccgcctcg
     2401 tgccgcgcat cggcgccgct ttcaccgccg tcgccgccac ccgcttcaac cgcccgggag
     2461 acgcatcatg atgcgcccgc ttcgcctcgt gcccgacggc accgccatca aattcatgcg
     2521 cggacgcttc gccgggctca tcacctcggc cgttctgagc ctcgcctcgg tggtcctgtt
     2581 cttctatccc ggcctcaact atggcgtcga cttcaagggc ggcgccatcg tcgagatccg
     2641 cttgcccgcg ccggccgatc tcgccgccat gcgcgccgct ttcgacggcc tcggattcgg
     2701 cgaggtccag ctccagcaat tcggcgcacc cgaggatgtg ctggtgcggc tcggccagca
     2761 ggcgcagggc gagacggcgc ggaagagcat ccaggacatg ctcgccctga actttcccgg
     2821 cagcgagatc cgccgtctcg agatcgtcgg cgcgcgggtg agcgacgagc tcttccgcaa
     2881 cggcatgctc gccaccgccc tcgctctcgc cgcggtgctg gcctatatct gggtcaggtt
     2941 cgactggcag ttcggcgtgg gcgtcgtcgg cacgttgctg ctcgacgtca ccaagacggt
     3001 gggcttcctc gcgctcgcac ggctgcaatt cgacctcaac gccgtcgccg ccatcctgct
     3061 gctgatcggc tattccgtca ccgacaaggt cgtcgtctac gaccgcatcc gcgagaacct
     3121 gcgcaagttc aagaccatgc cgctacgcga gctgatcgac ctcagcatca atcagacgct
     3181 cggccgcacc gtcgccacgt cgctcaccgt gctgctctcg atcctgccgc tcgcggtgat
     3241 cggcggcgag gccatccgcg gctttgccct catcatcatc ttcggcatcg tcgtcggcac
     3301 ctcgtcctcg atcttcatcg ccgcgccgat cctcttgttc
//
LOCUS       DASYNS010000340         6440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1647835, whole genome shotgun sequence.
ACCESSION   DASYNS010000340 DASYNS010000000
VERSION     DASYNS010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1647835"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VGP50_10625"
     CDS             complement(<1..1087)
                     /locus_tag="VGP50_10625"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEV8027875.1"
                     /translation="MMTSIFGGSSWFGWRMRAAPLALLLITGVPAERSDAVPVSGAQT
                     SLPRGTELGEDALDRPREAFRSEAAGGAKSYLVDLGDLAFNAPTLLGGAARQAGMSCG
                     TCHVNGVTNPRFYVPGLSTRPGTFDTTGHLFNPKADDGMLDALAIPSLRGARALAPYG
                     HDGRTASLRDFVRGVIVIEFAGPEPAPAILDAMVAYIDDIDFLPNPRLAADSRLTPNA
                     SAAERRGEALFARPFPQDPSLSCAACHVPPGAFVDHRRHDVGSGGFFKTPTLRNANFN
                     APYFHDGRYATYDEVVGHFDRAFALALSAQDKSDLVAYLSAIGDGVRPYEREGSTAAL
                     KEIGDFASVLATAIPARDRDVIALAVET"
     gene            complement(1288..3168)
                     /locus_tag="VGP50_10630"
     CDS             complement(1288..3168)
                     /locus_tag="VGP50_10630"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV8027876.1"
                     /translation="MSARSSARELKQGRPRRIARCVLAAGLLVIASCSPSPDRDHETA
                     TPIKHLIVLIGENRSFDHVFGLYRPLPGQTIHNLLSEGIVDASGAPGPNFARAAQFQA
                     APQPSYYVAAPAKTPYVTLPTPDLAGTPVAPSDAAPPFPSAAAVAAIEPALDAADRVL
                     LTTGASGLAARRGPDTRIARDGALPNGPFQLTGPRLPYDSYAGDTTHRFFQMWQQSDC
                     QVAQATPANPSGCLDDLYPFIASGVARRPNGNAMAFYNVNAGDAPFLKQLADTFTMSD
                     NFHQPLMGGTGANHQMLGLGDTLFWTDGHGNPVPPPAALIADPNPLPGTNDYTVDGNW
                     TNCSDAAQPGVGPIVSYLAALGLSANCAPGRFYMINNVAPAFLPNGAARSTGIFAPPS
                     ALRTIGDALNDRSISFVYYAGGFDAAVRAANGSTDPVDAIAAVYCQTCNFLQYATAIM
                     GDPAQRDAHLKDITDFFADLAAGTLPAVSFVKPDGFLDGHPASSKLDLFEALAKRILD
                     RLGAHPALERDTAVFIVFDEGGGYYDSGYIQPLDFFGDGPRIPFLVASPFARGGRVVH
                     TYEDHVSILKFIERNWHLAPLTARSRDNLPNPIPRPGNPYAPRNTPAIGDLFDMFDFS
                     SE"
     gene            complement(3262..3684)
                     /locus_tag="VGP50_10635"
     CDS             complement(3262..3684)
                     /locus_tag="VGP50_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8027877.1"
                     /translation="MAEPSVASAASNRSKIVVGVDDMPESLGLIDHIISDAGYIFMGA
                     AGGTECLALVARIVPQLILLDVEMPKMDGFATCKLLRADGRLTHVPIAFLTARKTSED
                     VRKGMAAGGNDFVLKPFDRAKLIERLNYWTRHRVNKRW"
     gene            complement(3697..5880)
                     /locus_tag="VGP50_10640"
     CDS             complement(3697..5880)
                     /locus_tag="VGP50_10640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8027878.1"
                     /translation="MAKQPVIRPDEVSEEIGVVFKTPLGLLASTANAGLCVAVLWHTI
                     PTALLLGWAVALAACLTARGWLWRAFHRRRPDAAGTARWGRWYALGAFVTGCLWGALA
                     LVVLMTDDVLYHGFVVMVLAGMGAGSVAADVAYLPAFYAFLLPSGLPLALALLMRGGV
                     VYTAMSIMVVVFLLTTMVFGRGIHRSLAANIRLISDKTRVVDELARARDAAETAHRAK
                     SAFLANMSHEIRTPMHGIIGMINLLLQSPLDDRQREHAELVRELTDSLLTIINDILDV
                     SKLEAGRLDLEVIDLDIAEVVRQVVDLMGPKAVQKGLVIRSDIDERARRTLRGDPTRI
                     RQILLNLVSNAIKFTETGGIIVRVECSELYSDEVALRIEVTDTGIGIPDQVVGNLFTK
                     FTQADQSVARRFGGTGLGLAISRQLVEAMGGKIGVESRPGAGSKFWLTLRLAVAVAPT
                     SQALAAQALAAPPPSAAPKSGSGKRVLLAEDILINQIIAIEMLKSAGYAVDVADNGIE
                     AVDAVQHQPYDVVLMDVHMPSMDGLDATRKIRELPAPRGRVPIVALTADAVAGVREQY
                     LAAGMDDFLSKPFNRKELLTVVERWCAERPAPASRPASAMPPESGAILDHAKIRELEE
                     VMPREDFQNLLTAWLDATRDRVGQIVKLAQGDNLPEIRLHAHNLVSTAGGVGAMQLSG
                     LARQLEEACHGGKTEAAQDLARRLGEVAGPSRDAIRAYLAPINVA"
     gene            complement(6205..>6440)
                     /locus_tag="VGP50_10645"
     CDS             complement(6205..>6440)
                     /locus_tag="VGP50_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEV8027879.1"
                     /translation="RGLAQSLARELAPKNIHVGHFVIDGGIRQAGDTRGGQPGDDALL
                     DPEAIAASYLHVHRQHRSAWTWEMELRPWVEKF"
BASE COUNT         1069 a         2027 c         2277 g         1067 t
ORIGIN      
        1 tcgtctccac cgcaagagca atgacgtccc ggtcccgcgc gggaatcgcg gtggccagca
       61 cgctcgcgaa atcgccgatc tccttgaggg ccgcggtcga accttcgcgc tcatagggcc
      121 tcacgccgtc gccgatcgcg ctgagatagg cgacgagatc gctcttgtcc tgagccgaga
      181 gtgcgagagc gaacgctcga tcgaaatgcc cgaccacctc atcgtatgtt gcatagcgcc
      241 cgtcatgaaa atagggggcg ttgaagttcg cgttgcgcaa tgtcggtgtc ttgaagaagc
      301 cccccgagcc gacgtcgtgc cggcggtgat cgacgaaagc gccgggaggc acatgacacg
      361 cggcgcagct caagcttgga tcttgcggga aaggtctcgc aaagagcgcc tcgccgcggc
      421 gctcggcggc gctcgcgttt ggcgtgagcc ggctatcggc ggcgagtcgc gggttcggca
      481 ggaagtcgat gtcatctata taggcgacca tggcgtcgag aatcgccggc gccggctccg
      541 gcccggcaaa ctcgatgacg atgacgccgc ggacgaagtc gcgaagcgac gcggtgcgcc
      601 cgtcatggcc gtaaggcgcg agcgcgcgcg ctccgcgcag gctcgggatc gccaacgcgt
      661 cgagcatgcc gtcatcggct ttgggattga agaggtgccc ggtcgtatcg aaggtccccg
      721 gccgagtcga gagtcccggc acatagaatc tcgggttcgt gacgccgttg acatggcagg
      781 tgccgcagct catgccggcc tggcgcgcgg cgccgcccaa cagcgtcggc gcattgaagg
      841 cgaggtcccc caggtcgacg agataggact tggcgccgcc cgccgcttcg gaccggaaag
      901 cctcgcgcgg gcggtcgagc gcatcttcgc cgagctcggt tcctcgcggc agagaggtct
      961 gcgcgccgct taccggaacg gcatcgctac gctcggccgg cacgcccgtg atcagcaaca
     1021 gggccaacgg cgctgcacgc atccgccagc caaaccagct gctgccgccg aaaatgctcg
     1081 tcatcatccg ccctgcgtgt ccgcggccgc acgccggccg cttcggcacg cagatttagc
     1141 ctcggcggct tttcgttttt atgacagcgg tcgagacatt cgccagatgg tgcccgcgac
     1201 ccggcgtcgg gtaaggcgtg cccaagcccg aaaactactc tcgtgggcgc atacgcccgt
     1261 cagggttgcg gcgcatggcg ctcacaatca ttcgctactg aagtcgaaca tgtcgaagag
     1321 atcgccgatc gccggcgtgt tgcgcggcgc atagggattg ccgggccgcg gaatcggatt
     1381 cggcaagttg tcacggctgc gcgcggtgag cggtgcgaga tgccagttgc gctcaatgaa
     1441 tttcagaatc gacacatggt cttcataggt atgcacgacg cgtccgccgc gagcgaaggg
     1501 cgacgcgaca aggaagggaa tgcgcggccc gtctccgaag aagtcgagcg gctgaatgta
     1561 gcccgaatcg taatagccgc cgccctcgtc gaagacgatg aagacggcgg tatcgcgctc
     1621 gagcgccgga tgggcgccga gccgatcgag aatacgcttt gccagcgctt cgaaaaggtc
     1681 gagcttcgag ctcgcgggat gtccgtcgag gaagccgtcg ggcttgacga aggacaccgc
     1741 gggcagcgtg ccggcggcga gatcggcgaa gaaatcggtg atgtccttga gatgagcgtc
     1801 gcgttgcgcc ggatcgccca tgatcgcggt cgcatattgg agaaagttgc aggtctggca
     1861 atagaccgcg gcgatcgcat cgaccggatc cgtggagccg ttcgccgctc tgacggcggc
     1921 gtcgaagccg cccgcgtagt agacgaagga gatgctcctg tcgttgagcg cgtcgccgat
     1981 ggtgcggagc gccgaggggg gcgcgaagat accggtgctt cgcgctgcgc cattcggcag
     2041 aaacgccgga gcgacattgt tgatcatata gaagcgaccc ggcgcgcaat tggctgagag
     2101 ccccagcgcg gcgaggtagc tcacgatcgg cccgacaccc ggctgcgccg cgtcggagca
     2161 gttggtccag ttgccgtcga ccgtgtagtc gttggtcccg gggagcgggt tgggatcggc
     2221 gatgagggcg gcgggcggcg ggaccgggtt cccgtggccg tcggtccaga agagcgtgtc
     2281 gccgaggccc agcatctgat ggttggcgcc ggtgccgccc atcagcggct ggtggaagtt
     2341 gtcgctcatc gtgaaggtgt cggcgagctg cttgaggaag ggcgcgtcgc cggcgttcac
     2401 gttgtagaac gccatggcgt tgccattcgg ccgccgcgcg acgccgctgg cgatgaaggg
     2461 atagagatcg tcgaggcagc cgctgggatt ggcaggagtc gcctgggcga cctggcaatc
     2521 ggattgttgc cacatctgaa aaaagcggtg cgtggtgtcg ccggcatagg aatcgtaagg
     2581 caggcgcggg ccggtcagtt ggaacggccc gttgggaaga gccccgtccc tggcgatgcg
     2641 tgtgtcaggc ccgcgccttg ccgcaagccc gctggcgccg gtggtgagca gcacccggtc
     2701 cgcggcgtcg agcgcgggct cgatggccgc gacagcggcg gcgctgggga agggcggtgc
     2761 ggcgtcgctc ggcgcgaccg gcgtgccggc gaggtcgggc gtcggcagcg tgacgtaagg
     2821 cgtcttcgcc ggcgccgcga cgtaatagct cggctgcggc gcggcctgga attgtgccgc
     2881 gcgcgcgaag ttgggtcccg gcgcgccact ggcgtcgacg atgccttcgg agagaaggtt
     2941 gtgaatcgtc tggccgggga gcggccgata gaggccgaag acatggtcga agctgcggtt
     3001 ctcgccaatc agcacgatga ggtgcttgat cggggtcgcg gtctcgtgat cccgatcggg
     3061 cgatggagag caggacgcga tgacgagaag tcccgcggcc aagacgcagc gtgcaatgcg
     3121 ccgcggccgt ccttgcttca gttctcgtgc cgaagagcgt gccgacatcc tctccccgcc
     3181 gggagcgtat cgcgcccgaa acggccgcgc acgccccctt tgggagcggg aacatagcag
     3241 ggttcggcgg gacgcccggc gctaccagcg cttgttgacg cgatgccgcg tccagtagtt
     3301 gaggcgctcg atcagcttgg cgcggtcgaa gggcttgagg acgaaatcgt tgccacccgc
     3361 ggccatgccc ttgcgcacgt cctcgctggt cttgcgggcc gtcaggaacg caatcggcac
     3421 atgggtgagc cgcccgtcgg cgcgcagcag cttgcaggtc gcgaagccgt ccattttcgg
     3481 catctcgacg tcgagcagta tcagctgcgg cacgatgcgc gcgaccagcg ccaggcattc
     3541 ggtgccgccc gccgcgccca tgaatatgta gccggcgtcg ctgatgatgt ggtcgatcag
     3601 accgaggctt tccggcatgt cgtcgacgcc gacgacgatc ttgcttcgat tgcttgccgc
     3661 tgacgcgacg gacggttcgg ccatggcgga tctcggctat gcgacgttga tcggcgcgag
     3721 ataggcgcgg atcgcgtcgc ggctggggcc ggcgacctca ccgagtcggc gcgcgagatc
     3781 ctgtgctgcc tccgtcttgc cgccgtggca tgcttcctcg agctgacgcg caaggcccga
     3841 gagctgcatc gccccgacgc cgcctgccgt gctgacgagg ttatgggcat gcaggcggat
     3901 ctcgggaagg ttgtctccct gcgccagctt cacgatctgg ccgacccggt cgcgcgtggc
     3961 atcgagccac gcggtaagaa gattctggaa gtcctcgcgc ggcatgactt cctcgagctc
     4021 gcggatcttc gcatgatcga ggatggcgcc gctctcgggc ggcatcgcgc tcgctggcct
     4081 ggacgctggc gccggacgct cggcgcacca gcgttcgacg accgtcagga gctctttccg
     4141 gttgaaaggc ttcgacagga agtcgtccat gccggcagcg aggtattgct cgcgcacgcc
     4201 ggcgacggca tcggcggtga gcgcgacgat gggcacccgc ccgcgcggcg ccggcagctc
     4261 gcggatcttg cgcgtcgcgt cgaggccgtc catcgacggc atgtggacgt ccatcaggac
     4321 gacgtcatag ggctgatgct gcacggcatc gaccgcctcg atgccattgt cggcgacatc
     4381 gacggcgtag ccggcgctct tcagcatctc gatggcgatg atctggttga tcaggatgtc
     4441 ctcggccaga agcacgcgct tcccgctgcc cgacttcggc gcggccgatg gcggcggcgc
     4501 ggccagtgcc tgtgcggcga gcgcctgcga ggtcggggcg acggcgacgg ccagccgcag
     4561 cgtcagccag aacttgctgc ccgcgcccgg ccggctctcg acgccgatct tgccgcccat
     4621 ggcctcgacc agctgccgcg agatggcgag gccgaggccc gtgccgccaa agcgccgggc
     4681 gacgctctgg tcggcctgcg tgaacttggt gaagaggttg ccgaccacct ggtccggaat
     4741 gccgatgccg gtgtcagtga cctcgatgcg cagcgcgacc tcgtcgctgt agagctcgct
     4801 gcactcgacc cgtacgatga tgccgccggt ctcggtgaac ttgatcgcgt tgctcacgag
     4861 gttgagcagg atctgccgga tccgcgtcgg atcgccgcgc aaggtacggc gggcccgctc
     4921 gtcgatgtcg gagcggatca cgaggccctt ttgcaccgcc ttcgggccca tgaggtcgac
     4981 gacctggcgc acgacttcgg cgatgtcgag gtcgatcact tcgagatcga gccgtcccgc
     5041 ttccagcttc gagacgtcga gaatgtcgtt gatgatggtg agcaggctgt cggtcagctc
     5101 gcggacgagt tcggcgtgct cgcgctggcg atcgtcgagc gggctctgaa gcagcaggtt
     5161 gatcatgccg atgatgccgt gcatcggcgt gcggatctca tgactcatgt tggcgaggaa
     5221 ggcggatttg gcgcgatgcg ccgtctccgc cgcgtcccgc gcccgggcga gctcgtcgac
     5281 cacccgggtc ttgtcggaga tgaggcgaat attggcggcg agcgagcggt ggatcccgcg
     5341 tccgaatacc atcgtcgtca gcaggaacac gacgaccatt atgctcatgg cggtgtagac
     5401 gacgccgccc cgcattagca aggcgagggc gagaggcagt ccggagggca ggaggaaggc
     5461 gtagaaggcc ggcagatagg ctacgtcggc ggccaccgag cccgctccca tcccggcaag
     5521 caccatgacg acgaagccat gatagagcac gtcatcggtc atcagcacga cgagagccag
     5581 cgcgccccat aagcagccgg tcacgaaagc gccgagcgcg taccaccgcc cccagcgcgc
     5641 cgtgccggca gcatccggcc ggcgccggtg gaaggcgcgc cacaaccatc cgcgggcggt
     5701 gaggcaggca gcaagcgcaa ccgcccagcc gagaagcagc gccgtcggaa tcgtatgcca
     5761 cagcacggcg acgcacaggc cagcgttggc ggtcgacgcc aacagcccga gcggggtctt
     5821 gaagacgacg ccgatctcct cggatacctc gtcgggtcgg attaccggtt gcttggccat
     5881 gacgaatcca gcgcacacca cgcccactgg ggcaccctcg atataagtga attcgtttaa
     5941 tggctgctta cgttcggacc cgatgccacg atttggcgca ggaatgccgc caaagttgca
     6001 caatttgtaa ccttgaggag cggttggcgc gggccgcgga aggtcgtcct cgctgcaacg
     6061 cagatatgcg ctcggcgcac ttgctgcact gcggtcacgc ggccatatgc ggcgcgaacg
     6121 ccttccccta ggcgtttcct ccctgaactt gggccgtgcc agcgcacggc ccttttttct
     6181 tcggcgctgc ggcgtcgcgc ggcgtcaaaa tttctcgacc cagggccgca gctccatctc
     6241 ccaggtccag gcgctgcggt gctggcgatg gacatggaga taggaggcgg cgatggcctc
     6301 ggggtcgagg agcgcatcgt ctcccggctg tcccccgcgg gtgtcgccgg cctggcggat
     6361 gccgccgtcg atgacgaaat ggccgacatg gatgtttttg ggcgcaagct cgcgggcaag
     6421 gctttgcgcg aggccgcgca
//
LOCUS       DASYNS010000341         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5232144, whole genome shotgun sequence.
ACCESSION   DASYNS010000341 DASYNS010000000
VERSION     DASYNS010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5232144"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VGP50_10650"
     CDS             <1..410
                     /locus_tag="VGP50_10650"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEV8027880.1"
                     /translation="NAPAFSAAILTARAAEKGNSTDYDTKVGAQQKQGYPDLAPVTTT
                     LAPAQAYERALAAAQGMSGWRIVTNDPADGRIEASQSTLFMGFTDDVVIRVTPEGSGS
                     RIDMRSESRQGISDFGVNAGRVRRYVAALKAQL"
     gene            545..1294
                     /locus_tag="VGP50_10655"
     CDS             545..1294
                     /locus_tag="VGP50_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8027881.1"
                     /translation="MTGRLAGKKALVTAAAQGIGRAAALAFAAEGASVVATDINLAKL
                     EELGPAGAGKIEIRRLDVTDAAAIAVLAQTLGAIDVLFNCAGFVHHGTILDCAETDWD
                     ATLDVNVRSMYRMIRAFLPAMLQRRQGASIVNMASAVSSVKGAPNRFVYGTSKAAVIG
                     LTKSVAIDFIKDGIRCNAICPGTVETPSLGERIAAQGGGPEVRQAFIARQPVGRLGTP
                     EEIAALALYLASDESRFTTGAIHIIDGGWTI"
     gene            1315..>1707
                     /locus_tag="VGP50_10660"
     CDS             1315..>1707
                     /locus_tag="VGP50_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV8027882.1"
                     /translation="MKLLRWGPRGQEKPGMLDRDGVLRDLSGAVADINPETLAPAALD
                     KLRKLDPAKLPAVPGKPRLGPCVAQVPKLVCVGLNYVDHAKEAGMQVPGEPVLFMKAT
                     TAISGPNDEVMLPKGAQKGDWEVELGIVI"
BASE COUNT          291 a          568 c          585 g          263 t
ORIGIN      
        1 agaacgcgcc ggccttctcc gccgcgatcc tgacggcgcg cgcagccgag aagggcaaca
       61 gcaccgatta cgacaccaag gtcggcgcgc agcagaagca gggctatccc gatctcgcgc
      121 cggtgacgac gacgctggcg ccggcgcagg cctatgagcg cgcgctcgcc gcggcgcaag
      181 gcatgtcggg ctggcgcatc gtcaccaatg atcccgcgga cgggcgcatc gaggcgagcc
      241 agtcgacgct gttcatgggc tttaccgacg acgtcgtcat ccgcgtcacg cccgagggat
      301 cgggaagccg catcgacatg cgttccgaga gccgccaggg catcagtgat ttcggcgtga
      361 acgccgggcg cgtccgtaga tatgtggccg cgttgaaggc gcagttgtag ccgggtctcg
      421 atcaggtgga attctgcgcg gtcccctcac cctcccactg cttcgctgaa gcttcgcagt
      481 gggtccctcc ctctcccgcg gtgcgggaga ggggctattt gcagccgtga tggagaggcg
      541 agcgatgacg gggagactcg ccggcaagaa ggcgctggtg acggcggcgg cgcagggcat
      601 cggccgtgcc gcggcgctcg cctttgcggc cgaaggggcg agtgtcgttg ctaccgatat
      661 caacctcgcg aagctcgagg agctggggcc ggcgggtgcg ggaaagatcg agatccggcg
      721 tctcgacgtc accgatgcgg cggcgatcgc cgtgctcgcg caaacgctgg gcgcgatcga
      781 cgtgctgttc aattgtgccg gcttcgtcca tcacggcacc atcctcgatt gcgccgagac
      841 ggactgggac gcgacgctcg acgtcaatgt ccgctccatg tatcgcatga tccgcgcttt
      901 tctcccggcc atgctgcagc gccgccaggg cgcttcgatc gtcaacatgg catcggcggt
      961 ctcgtcggtg aaaggcgcgc ccaaccgctt cgtttacggc accagcaagg cggcggtcat
     1021 cggcctcacc aagtcggtcg ccatcgattt catcaaggac ggcatccgct gcaacgccat
     1081 ctgccccggc accgtcgaga cgccgtcgct cggcgagcgc atcgccgcgc agggcggcgg
     1141 tcccgaagtg cgccaggcct tcatcgcgcg ccagccggtg ggccgcctcg gcacgccgga
     1201 agagatcgcg gcgctcgccc tttacctcgc gagcgatgaa tcacgcttca ccaccggcgc
     1261 catccacatc atcgacggcg gctggaccat ctgacaaagg ggaggaaccg agagatgaag
     1321 ttgcttcgct ggggcccgcg cggccaggag aaaccgggca tgctcgaccg cgacggcgtg
     1381 ctgcgcgatc tctcgggggc cgttgccgac atcaatcccg agacgctggc gccggccgcg
     1441 ctcgacaagc tcagaaagct cgacccggca aagctgccgg cggtccccgg caagccgcgg
     1501 ctcggtcctt gcgtcgcgca ggtgccgaag ctcgtctgcg tcggcctcaa ctatgtcgac
     1561 cacgccaagg aagccggcat gcaggtgcca ggcgagccgg tgctgttcat gaaagcgacg
     1621 acggcgatca gcgggcccaa tgacgaggtc atgctgccca aaggcgcgca gaagggcgat
     1681 tgggaggtcg agctcggcat cgtcatc
//
LOCUS       DASYNS010000342         7407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5544623, whole genome shotgun sequence.
ACCESSION   DASYNS010000342 DASYNS010000000
VERSION     DASYNS010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5544623"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VGP50_10665"
     CDS             complement(<1..240)
                     /locus_tag="VGP50_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV8027883.1"
                     /translation="MTGAFKAACIQLSSGREVEPNIAALGDLVRRARAAGAELIMTPE
                     VSDMMEPKRALRLAKAKDEATHPMLAACRALARETG"
     gene            complement(237..503)
                     /gene="grxC"
                     /locus_tag="VGP50_10670"
     CDS             complement(237..503)
                     /gene="grxC"
                     /locus_tag="VGP50_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEV8027884.1"
                     /translation="MARVEIYSTMFCGYCARAKSLIEKKGVDYFDIDIIEDASKRDEM
                     VQRSGGRQTVPQIFIDGEHIGGSDDLYALERAGKLDAKLGIERP"
     gene            complement(583..1386)
                     /locus_tag="VGP50_10675"
     CDS             complement(583..1386)
                     /locus_tag="VGP50_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV8027885.1"
                     /translation="MRPRWQARVATADHTDDWLMTYADMITLLLCFFAIFLSISITKK
                     DAAPKVEMAQPAPAPAPVRQVIFLNGRLPFPDLERSDEATEPPPAAKTTPADAAPELE
                     IVSPFEVAAMPAASEASDAKPEGDRITMLEFGSAAFFESGSATLSRSGEAILRKVAAD
                     LGAGAFDDYRITVEGHTDDTPIKTAQFPSNWELSTARASAVVHFLLDHGIPAARLRAA
                     GYADTFPKAPNRDAAGNAMPQNQAQNRRVVIKLEKIDKAEPSAAMRRPT"
     gene            complement(1383..2258)
                     /locus_tag="VGP50_10680"
     CDS             complement(1383..2258)
                     /locus_tag="VGP50_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEV8027886.1"
                     /translation="MPNFCRRFIKSHACAPVMGAVFGFLFILGAIVLSTRSLLAFFSI
                     EGLAIVVGGVIAVAFMSFEAEDVRKALDVVAGMFRRPADAAPDTLHHDMIEIIGWAYM
                     VKEKGMRRLESSLAKRGIADPFVKYGLNMVVSGYTPEEVRAMMETAADAALERESIPA
                     DVLQSMASHAPAFGMIGTLVGMVAMLCALSDNVANIGSSLSVSFLSTLYGVVSARMVY
                     MPAAAKLRQSISKRRFRNQLITEGMVMLVSDKTLMYIQDRLNSFLRPELHNYLDQFRG
                     AAATAAPPRLKVVRA"
     gene            complement(2520..3221)
                     /locus_tag="VGP50_10685"
     CDS             complement(2520..3221)
                     /locus_tag="VGP50_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV8027887.1"
                     /translation="MLDVLLPPRCLSCGTEVSAAGTLCAECWRGITFLGAPCCACCGL
                     PFEFALGPGTLCGACAQAHPPFARARAVMRYDEASRPLVLAFKHGDRLHLAPALGAWM
                     QRAGAELLAAADFLMPVPLHWTRLFARRYNQSAVLAHAIHAAGGPPVAPDWLIRRRRT
                     PSQGKRNALARARNVRSAFALRHDRDVRDRHLVLIDDVFATGATVGECARVLRRAGAR
                     QIDVLTLARTVRQEG"
     gene            complement(3302..4201)
                     /locus_tag="VGP50_10690"
     CDS             complement(3302..4201)
                     /locus_tag="VGP50_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV8027888.1"
                     /translation="MDWDDLRLVLAIARGRSLSGAARALGVTHSTVFRRLDAIERRTG
                     ARLFERFRDGYAPTAAGDAMATVAARLAEEVLALERRLAGQDLRPSGSVRLTTTDTLA
                     LAVVMPHLAAFQEAYPEIRLELAITNVVADLTRRDADVALRPTPDPPATLMGRRLAAI
                     AYAIYGAPEYLSRHRGREWSSHDWIGREDALGSTVAARWMRENVPEERIACRLDAVSV
                     MREAARAGMGLVVLPCYFGDGAPGLRRAQEKPLADARSALWLLTHRDLRHTARIRALM
                     DFLASRLTAERPRIEGRQPAGSR"
     gene            4306..4497
                     /locus_tag="VGP50_10695"
     CDS             4306..4497
                     /locus_tag="VGP50_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027889.1"
                     /translation="MRRLNSRDIDGGCAEVAVPPVWWPLVIVAVGAAAMLLLLGSVSF
                     EGLDRLVPEGQPFLPYFTT"
     gene            4550..5473
                     /locus_tag="VGP50_10700"
     CDS             4550..5473
                     /locus_tag="VGP50_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV8027890.1"
                     /translation="MPLETDPLRVFDLRALRRHRARASRRAEAADFLFAESAERLTDR
                     LDDVARRFPLALDLGSRHGVLARALGARGGIEMLVRADRTLELLRQGPEPARAVVVEA
                     EALPFAPGSFDLVLSNLDLHWVNDLPGALAQLRRMLKPDGLLLAALLGGGTLGELRRA
                     LLEAELAEEQGASPRVSPFADPADLGALLQRAGFALPVVDSDIIEVTYPDAFALMRDL
                     RAMGETNAVVERRRRFTRRATLMRAATAYGALFGRADGRIPATFEIVTLTAWAPHESQ
                     PKPLPRGSAKTRLAAALEAADPRPAGGDKSP"
     gene            5543..6172
                     /locus_tag="VGP50_10705"
     CDS             5543..6172
                     /locus_tag="VGP50_10705"
                     /inference="COORDINATES: protein motif:HMM:NF040005.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HEV8027891.1"
                     /translation="MLASLTLAAAAAGAPAHAAPQVYTYRVIHAVYGEIGTYTNVVEQ
                     TGEDTRVDSELHIAVKMLGITMFREEARRSEYWRNQRLVSFDGVTVTNGDKTEIRGEA
                     RDGGFAVITASGTYLAPAEIHPSNPWCPMILNADVMMSTRTGKVEHVRVTGGEKEPVA
                     FNGDTQRLHQYEIVSDQHQFVWLDDNGVTVAFRTEEDGSPVDFVLVSKS"
     gene            complement(6272..6694)
                     /locus_tag="VGP50_10710"
     CDS             complement(6272..6694)
                     /locus_tag="VGP50_10710"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEV8027892.1"
                     /translation="MAATEAEPLPVILVVAVALVDTDGRVLLAQRPPGKAMAGLWEFP
                     GGKVQANEAPEAALIRELNEELGIDVAEVCLAPLTFASHRYERFHLLMPLYVCRRWNG
                     TVTAREGQALAWVRPLKLAGYPMPPADKPLVAMLQDLL"
     gene            complement(6675..>7407)
                     /locus_tag="VGP50_10715"
     CDS             complement(6675..>7407)
                     /locus_tag="VGP50_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HEV8027893.1"
                     /translation="RRAEIDGAPVTINGFAKGSGMIAPDMATMLAFLFTDAALPAKLL
                     QPLLAAANERSFNSITVDGDTSTSDTALLFATGKAGHKRVTRPADPRLKSFRRALDEV
                     MIDLAQQIVRDGEGAEKFVTIEVKGAASAKAGRRIGLAIGNSPLVKTAIAAGDANWGR
                     IVMAVGKSGEKADRDTLAVSVGGVRIAEKGGPVPGYDEAPVAKHMAGREIHIQVDLGI
                     GNGRATVWSCDLTHRYIDINGSYRS"
BASE COUNT         1173 a         2480 c         2534 g         1220 t
ORIGIN      
        1 gccggtctca cgcgccagcg cgcggcaggc ggcgagcatg ggatgcgtgg cctcgtcctt
       61 cgccttggcg aggcgcagcg cgcgcttcgg ctccatcatg tcgctcactt cgggcgtcat
      121 gatgagctcg gcgccggccg ctcgcgcgcg gcggacgaga tcgccgagcg cggcgatatt
      181 gggctcgacc tctcggcccg aggagagctg gatgcaggcc gccttgaagg cgccagtcac
      241 gggcgttcga tccccagctt ggcgtcgagc ttgccggcgc gctccagagc ataaaggtcg
      301 tcgctgccgc cgatatgctc gccatcgatg aagatttgcg gcaccgtctg ccgtccgccc
      361 gagcgctgga ccatctcgtc gcgcttcgag gcgtcctcga tgatgtcgat gtcgaagtag
      421 tcgacgcctt tcttctcgat caggctcttc gcgcgcgcac aatagccgca gaacatcgtc
      481 gaatagattt ccacccgcgc catgaccgtt ctcgctgtcc agtccgccgt cccaccctcc
      541 agcatttagc caaaaaggcg cggaatcaag cgtgttctcg ccctatgtgg gccgccgcat
      601 cgcggcgctc ggttccgcct tgtcgatctt ctcgagcttg atgacgacgc ggcggttctg
      661 agcctggttt tgtggcatgg cgttgccggc ggcatcgcgg ttgggcgcct tggggaaggt
      721 gtcggcatag ccggcggcgc gcagccgcgc tgcgggaatg ccgtggtcca gcaggaaatg
      781 caccaccgcc gaggcgcgcg ccgtcgacaa ttcccagttc gagggaaatt gcgcggtctt
      841 gatcggcgtg tcgtcggtgt ggccctcgac ggtgatccga taatcgtcga acgcccccgc
      901 gccgaggtcg gccgcgacct tcctcaggat cgcttcgccg gaccggctga gcgtggccga
      961 gccgctctcg aagaacgcgg cgctgccgaa ttcgagcatc gtgatccgat cgccttcggg
     1021 tttggcgtcg ctcgcttccg acgccgcggg catggcggcg acctcgaacg gcgacacgat
     1081 ttcaagctcc ggagcggcgt cggcgggcgt ggttttcgcg gcggggggcg gctccgtcgc
     1141 ttcatcgctg cgctcgagat ccgggaaagg cagccttccg ttcaggaaga tcacttgccg
     1201 caccggcgcc ggagccggtg cgggttgtgc catctcgact ttcggcgcgg cgtccttctt
     1261 cgtgatcgag atcgacagaa agatggcgaa gaagcacagc agcagcgtga tcatgtcggc
     1321 ataggtcatg agccagtcat cggtatgatc ggccgtcgcg accctggcct gccagcgcgg
     1381 tctcatgcgc ggacgacctt gagacgcggc ggcgctgccg tcgcggccgc gccgcggaac
     1441 tgatcgaggt agttgtgcag ctcgggacgc aggaagctgt tcaggcgatc ctggatgtac
     1501 atgagcgtct tgtcgctgac cagcatcacc atcccctcgg tgatgagctg gttgcgaaac
     1561 cggcgcttcg agatgctttg gcggagcttc gctgcggccg gcatatagac cattcgcgcc
     1621 gagacgacgc cgtagagcgt cgagaggaag gatacggaga ggctcgagcc gatgttcgcg
     1681 acgttgtcac tgagcgcgca aagcatggcc accatgccga ccaaagtgcc gatcatgccg
     1741 aaggccggcg catggctcgc catgctctgc aacacatcgg ccggaatgct ctcgcgctcc
     1801 agggcggcat ccgcggccgt ctccatcatg gcgcggactt cctcgggggt gtagccgctc
     1861 accaccatgt tgaggccgta tttgacgaag ggatcggcaa tgccgcgctt ggcgaggctc
     1921 gattcgagcc ggcgcatgcc tttttccttg accatgtagg cccagccgat gatctcgatc
     1981 atgtcgtgat gaagcgtgtc cggcgccgca tcggccggtc gcctgaacat tccggcgacg
     2041 acgtcgagcg ccttgcggac gtcctcggcc tcgaagctca tgaaggcgac ggcgatcacg
     2101 ccgcccacga cgatcgccag gccctcgatg ctgaagaagg ctaaaaggct gcgcgtgctc
     2161 agcacgatcg cgccgaggat gaacaggaag ccgaacaccg cccccatgac gggggcgcag
     2221 gcgtgggact tgatgaagcg ccgacagaag ttgggcatga ggttcctttg aggcgagacg
     2281 accggcgagc cttctcaagc aaccgtcatg ccatcggcgc gcttccgtta aggcattgat
     2341 ttgttgagca tttatttgcc cgacgtcccg ggccgcgggg gcgccgctcc gccctgagga
     2401 aattgtcgcc gggcggtccg acaaaatctg ccgggagcgc agatgcgcct tcggttacat
     2461 tttgcccgag ctggtggtct gtccctcgcg atcgatccgc gaccggcgcg ccgcagcgac
     2521 tacccttcct gccggaccgt gcgggcgagc gtcaggacgt cgatctggcg cgcgccggcg
     2581 cggcggagca cgcgcgcgca ttcgccgacg gtggcgccgg tggcgaagac atcgtcgatc
     2641 agcacgaggt gacggtcgcg cacatcgcgg tcgtgtcgga gcgcgaaggc gctcctgacg
     2701 ttgcgcgccc gtgcgagcgc gttccgcttg ccctgcgacg gcgtgcgccg gcgccggatg
     2761 agccaatcag gcgccaccgg cggtccgccg gccgcatgga tggcatgggc cagcaccgcc
     2821 gactggttgt agcggcgcgc gaaaagccgc gtccaatgca gcggcaccgg catgaggaag
     2881 tcggccgcgg ccaggagctc ggccccggcg cgctgcatcc aggcgccgag cgcgggagcg
     2941 agatggagcc ggtcgccatg cttgaaggcg agcaccagag gccggctcgc ctcgtcgtag
     3001 cgcatcacgg cgcgcgcccg ggcaaaaggc ggatgcgctt gcgcgcaggc gccacagagc
     3061 gtgccggggc cgagcgcgaa ttcaaaaggc aggccgcagc aggcgcaaca gggcgcgcca
     3121 aggaatgtga tgccgcgcca gcactcggcg cagagcgtac cggccgcgct cacctccgtg
     3181 ccgcaactga gacagcgcgg cggcagcagg acatccagca ccgcgccagc gtagcgtctc
     3241 agcgtcctcg tgcgggccag gctcgacatc gtcgcgatgt cagcccggcg ccgctctgct
     3301 gtcaacggct tcccgccggc tgccggcctt cgatccgcgg gcgctccgcc gtgagccggc
     3361 tggcgaggaa atccatgagg gcgcgaatac gcgcggtgtg acgaagatcc cgatgggtga
     3421 gcagccagag cgccgagcgt gcgtccgcga gcggcttttc ctgagcgcgg cggagtcccg
     3481 gggcgccgtc gccgaaatag cagggcagca ccacgaggcc catgccggcc cgcgccgcct
     3541 cgcgcatgac actcaccgcg tcgagccggc aggcgatgcg ctcctctggc acgttctctc
     3601 gcatccagcg cgccgcgacc gtgctaccga gcgcgtcctc gcgcccgatc cagtcgtggg
     3661 aggaccattc tctcccgcga tgccgtgaga gatactcggg cgcgccatag atcgcgtaag
     3721 cgatcgcggc aaggcgccgc cccatcagcg tggcgggcgg gtcgggcgtc ggacgaagcg
     3781 ccacatcggc atcgcggcgc gtgaggtcgg ccaccacatt cgtgatcgcg agctccagac
     3841 ggatttccgg ataggcctcc tggaaggcgg ccagatgcgg catcacgacc gccagcgcca
     3901 gcgtgtcggt ggtggtgagc cgcacgctgc ccgagggccg caaatcctgc ccggcgaggc
     3961 ggcgctcgag ggccagcacc tcttcggcga ggcgggcggc gaccgtggcc atggcatcgc
     4021 cggcggcggt cggcgcatag ccatcgcgga agcgctcgaa cagacgggcg ccggtcctgc
     4081 gctcgatggc atcgaggcgg cggaacaccg tcgaatgcgt gacgccgagg gcgcgcgcgg
     4141 cgcccgaaag cgagcggccg cgtgcgatgg ccagcacgag gcgcagatcg tcccaatcca
     4201 tcccgttgtg catttgcgca aactctgtgt gcataaatcg ccaatagctg ttcgataatc
     4261 gacaggctac atggcaacga tggagacggc aaaggaggat ttcccatgcg gcgcctgaat
     4321 tcgcgcgata tcgacggcgg ctgcgccgaa gtcgcggtcc ccccggtctg gtggccgctc
     4381 gtcatcgtcg ccgtgggcgc ggcggcgatg ctgctgctgc tcggcagcgt cagtttcgag
     4441 ggtctcgacc ggctggtgcc cgagggccaa cctttcctgc cctacttcac gacctgacgc
     4501 cgcggctccg gcgctggccc gagcggcgcg gccgtgccat atagaggcca tgccgctcga
     4561 gacggatccg ctgcgcgtct tcgacctgag ggcactgcgt cggcaccgcg cgcgcgcctc
     4621 tcgccgcgcc gaggcggccg acttcctctt cgccgagagt gcggagcggc tgaccgaccg
     4681 gctcgacgac gtggcacgcc gctttccgct tgccctcgat ctcggcagcc gccacggcgt
     4741 cctcgcccgc gcgctcggcg cacgcggcgg catcgagatg ctggtccgtg ccgaccggac
     4801 gctcgaactt ctgcgccaag gcccggagcc ggcgcgcgcc gtcgttgtcg aggcggaagc
     4861 gcttcctttt gcgccgggct ctttcgatct cgtgctcagc aatctcgatc tgcattgggt
     4921 caacgatttg ccgggcgcgc tcgcgcagct tcgccgcatg ctgaaacccg atggcctctt
     4981 gctggccgcg ctgctggggg gcggcacgct gggcgagctg cgccgcgcgc ttctcgaggc
     5041 cgagcttgcg gaggagcaag gcgcgagccc tcgcgtctcg cccttcgccg accctgccga
     5101 tctcggcgcg ctgctgcagc gcgccggctt tgcgctgccg gtggtcgaca gcgacatcat
     5161 cgaggtcacc tatcccgatg cgttcgcatt gatgcgcgac ctccgcgcga tgggcgagac
     5221 caatgccgtg gtcgagcggc gccgccgctt cacccgacgc gccacgctga tgcgtgccgc
     5281 caccgcctat ggcgcgcttt tcggccgcgc cgacgggcgc attcccgcaa ccttcgagat
     5341 cgtcacgctg accgcctggg cgccgcacga gagccagccg aagccgctgc cgcggggcag
     5401 cgcgaagacg cggctggcgg ccgctctcga agccgcggac ccgcggcccg ccggcggcga
     5461 caaatcgcca tgagcgccgg tgatttcgct cgcctttgac gcagcgcctc gatattttcg
     5521 aatcatggca agggtactcg ccatgctggc ctctctgacg cttgccgccg cggcggcggg
     5581 cgctcctgcc catgccgccc ctcaggtcta tacctatcgt gtgatccacg ccgtctacgg
     5641 cgagatcggc acctacacca acgtggtcga gcagaccggc gaagacacgc gcgtcgacag
     5701 cgagttgcat atcgccgtga agatgctggg catcaccatg ttccgagagg aggcgcggcg
     5761 tagcgagtat tggcgcaatc agcggctcgt ctccttcgac ggcgtgactg tcaccaacgg
     5821 cgacaagacc gagattcgcg gcgaggcgcg cgacggcggc tttgccgtca tcacggccag
     5881 cggcacctac ctggcgccgg cggagatcca tccgtcgaat ccctggtgcc cgatgatcct
     5941 caatgccgat gtcatgatgt cgacgcgcac cggcaaggtg gagcatgtgc gcgtgacggg
     6001 cggcgagaag gagccggtgg cgttcaacgg cgatacgcag cggcttcatc aatacgagat
     6061 cgtcagcgac cagcaccaat tcgtctggct cgacgacaac ggcgtgacgg tcgcttttcg
     6121 caccgaagag gacggcagcc cggtcgactt cgtgttggtc agcaagtcgt aggggaaagt
     6181 tgtcagttat cagtcatcag tttgtcgcag agccgtgccg cgggcttcac cactgatcac
     6241 tgacaactga caactgataa ctgacaactt cctaaagcag atcctgcagc atcgcgacga
     6301 gcggcttgtc ggcgggcggc atcgggtacc cagcgagctt caagggtcgc acccaggcga
     6361 gcgcctggcc ttcgcgcgcc gtgaccgttc cgttccagcg tcggcagaca tagagcggca
     6421 tcagcaggtg gaagcgctcg tagcgatggg acgcgaaagt gagcggcgcg aggcagactt
     6481 cggccacgtc gatcccgagc tcctcgttga gctcacggat cagtgccgcc tccggggctt
     6541 cgttcgcttg caccttgccg cccgggaatt cccaaagccc ggccatcgcc ttgccgggcg
     6601 gccgctgcgc cagcaacacc cggccatcgg tatcgacgag cgcaaccgcg acgacgagga
     6661 tgacgggaag cggctcagct tcggtagctg ccattgatgt cgatatagcg atgcgtcagg
     6721 tcgcagctcc agaccgtggc gcggccattg ccgatgccga gatcgacctg gatgtggatc
     6781 tcgcgcccgg ccatgtgctt ggccaccggc gcctcgtcgt agccgggcac cggcccgccc
     6841 ttctcggcga tgcggacgcc gccgacgctg accgcgagcg tatcgcgatc ggctttctcg
     6901 ccggacttgc cgacggccat gacgatgcgg ccccaattgg cgtcgccggc ggcgatcgcc
     6961 gtcttcacca ggggcgagtt gccgatggcg aggccgatgc gccggcccgc cttcgccgag
     7021 gcagcgccct tcacctcgat ggtgacgaat ttctcggcgc cttcgccgtc gcggacgatc
     7081 tgctgggcaa ggtcgatcat gacctcgtcg agcgcgcgtc gaaaggattt gaggcgaggg
     7141 tcggcaggcc gcgtgacgcg cttgtgcccc gctttgccgg ttgcgaaaag aagggcggtg
     7201 tcgctggtgg aggtgtcgcc atcgacagta atcgaattga acgagcgctc attggccgcg
     7261 gccagcagcg gctgaagcag cttcgccggg agcgccgcgt cggtgaagag gaaagcgagc
     7321 atcgtcgcca tgtccggcgc gatcatgccg gagcccttgg cgaagccgtt gatcgtcacc
     7381 ggcgcgccgt cgatctccgc gcgccgc
//
LOCUS       DASYNS010000343         3333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2269876, whole genome shotgun sequence.
ACCESSION   DASYNS010000343 DASYNS010000000
VERSION     DASYNS010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2269876"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VGP50_10720"
     CDS             complement(<1..605)
                     /locus_tag="VGP50_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8027894.1"
                     /translation="MLSLLPHIPSTQALFAQLTLGLINGAFYAMLSLGLAVIFGLLNI
                     INFAHGAQYMLGAFCTWLMLNYLGVGYWPALILAPIVVGLFGIALERLLLRHIYRLDH
                     LYGLLLTFGIALIVEGLFRQAYGTSGQPYDIPDQLSGAQHLGFMNLPNYRAWVIVASL
                     AVCFGTWYLIERTRLGAYLRAATENPQLVQAFGINVPRMVTL"
     gene            complement(881..2083)
                     /locus_tag="VGP50_10725"
     CDS             complement(881..2083)
                     /locus_tag="VGP50_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8027895.1"
                     /translation="MTLKILWTAVAAVALGAAGAAAQTAPVKIGVLTDMASLYADIGG
                     KGSLEAAKMAAEDAGPVLGQPVQVIGGDHQDKPDVGSSIARQWYDQEGVDVITDVPNS
                     GVALAVNEVSREKKKIALWTGPASSDLTGPKCNAYAASWVYDTYALAHVTGSAVVKSG
                     GDSWFFLTADYAFGHALERDTTAVVEASGGKVLGDVSVPLNNADFSSFLVQAQSSKAK
                     VIGLANAGGDTINSIKQGAEFGIVEGGQKFAALLMFISDVHSLGLKTAQGLELSAPFY
                     WDMNDETRAFSKRFSDRVGRPPTFTQAGVYSAIHHYLNAVKAINSKDPDKVMAKMRET
                     PINDFMTKNGKLREDGRVIRDFYLFEVKKPEESKAPWDYYKLLQSVSGDDAFRPLDKG
                     GCPLVTKG"
     gene            complement(2110..2832)
                     /locus_tag="VGP50_10730"
     CDS             complement(2110..2832)
                     /locus_tag="VGP50_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8027896.1"
                     /translation="MPDAAAPPPEMLRVEGLEAWYGESHVLHGMSFAVEQGQVVTLLG
                     RNGVGKTTTLRAVMGILPKRRGAVRFNGQELIHLPSNRIARAGIAYCPEERGIFASLS
                     VEENLLLPPIVQPGGMSVDEIYAIFPNLSERRKSQGTKLSGGEQQMLAIGRILRTGAR
                     LLLLDEPTEGLAPVIVQQIGALIRTLKTKGFTILLVEQNFRFAATVADRHYVVEHGRV
                     VDVIANDRLNDNIEKLQEYLGV"
     gene            complement(2825..>3333)
                     /locus_tag="VGP50_10735"
     CDS             complement(2825..>3333)
                     /locus_tag="VGP50_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8027897.1"
                     /translation="VRSFQISAVFPHLTVRENLRVALQRPLGISFHFWRSAKSLRALD
                     PRADALMTAVGLADDAESPATELPYGRKRALEIATTLALDPEMLLLDEPMAGLGHEDI
                     GRISDLIKDVARQRTVLMVEHNLSVVADLSHKITVLTRGHVLAEGSYAEVSANAEVRE
                     AYMGTGHA"
BASE COUNT          593 a         1025 c         1161 g          554 t
ORIGIN      
        1 agcgtcacca tgcgcggcac gttgatgccg aaggcctgca ccagctgcgg gttctccgtg
       61 gcggcgcgga gataagcgcc aagccgcgtc cgctcgatga ggtaccaggt gccgaaacag
      121 acggcgagcg aggcgacgat cacccaggcg cggtaattcg gcaaattcat aaagccgaga
      181 tgctgcgcac cggaaagctg gtcgggaatg tcatagggct ggcccgaggt gccataggcc
      241 tggcggaaca agccttcgac gatgagcgcg atgccgaagg tcagaagaag cccgtagaga
      301 tggtcgaggc gatagatgtg acgcagcaag aggcgctcga gggcgatacc gaagagaccg
      361 acgacgatcg gtgcaaggat cagcgccggc caatagccga cgccgagata gttcagcatc
      421 agccaggtgc agaaggcgcc cagcatgtac tgcgcgccat gggcgaagtt gatgatgttg
      481 agcaggccga agatgaccgc gaggccgagg ctcagcatgg cgtagaaggc gccgttgatg
      541 aggccgagcg tgagctgcgc gaacagcgcc tgggtcgagg ggatatgcgg caagagcgag
      601 agcatgctaa ggcactcgtg ccctcgcgcc agccctctcg atcacacgcc gaccgtgact
      661 ccctctccgc ccctcagggg cggagagggc cggggtgagg tggggcgccg acactggcag
      721 aatgccggcg tccttgccgg gagagaaaga gaaatcgagc aacgatatcg agcttattgc
      781 gcggcaccga ggcacggcac gccccacctc accccaaccc tctccgcccc tgaggggcgg
      841 agagggagaa gagttgtcgg ggagggggag cggcaaaggt tcagcctttt gtcaccagcg
      901 ggcagccgcc cttgtcgagg gggcggaagg cgtcgtcgcc ggagacgctc tggaggagct
      961 tgtagtagtc ccagggcgct ttcgactcct cgggcttctt gacctcgaag agatagaagt
     1021 cgcggatgac gcggccgtcc tcgcgcagct tgccgttctt ggtcatgaag tcgttgatcg
     1081 gcgtctcgcg catcttcgcc atgaccttgt cggggtcctt gctgttgatg gctttgacgg
     1141 cgttcagata gtgatggatc gcgctgtaga cgccggcctg ggtgaaggtg gggggacggc
     1201 cgacgcggtc ggagaagcgc ttcgagaagg cgcgcgtctc gtcattcatg tcccagtaga
     1261 agggggcgga gagctcgagg ccctgcgccg tcttgagacc gaggctgtgc acgtcgctga
     1321 tgaacatgag gagcgcggcg aatttctggc cgccctcgac gatgccgaac tcggcgccct
     1381 gcttgatcga gttgatggtg tcgccgccgg cattggcgag gccgatcacc ttggccttcg
     1441 acgactgcgc ctgaaccagg aaagacgaga agtcggcgtt gttgagcggc acgctcacat
     1501 cgcccaggac cttgccgccg ctggcttcga cgaccgctgt ggtatcgcgc tcgagcgcgt
     1561 ggccgaaggc gtagtcggcg gtgaggaaga accagctgtc gccgccgctc ttgaccaccg
     1621 ccgagcccgt cacatgggcg agcgcataag tatcatagac ccagctcgcg gcgtaggcat
     1681 tgcatttggg gccggtaagg tcggaggacg ccgggcccgt ccagagggcg atcttctttt
     1741 tctcgcgcga cacctcgttg acggcgagcg ccacgccgga attgggcacg tcggtgatga
     1801 cgtcgacgcc ttcctggtca taccactggc gggcgatgct ggagccgaca tcgggcttgt
     1861 cctggtgatc gccgccgatc acctgcaccg gctggcccag caccgggccg gcatcctcgg
     1921 ccgccatctt ggcggcctcg agagagcctt tgccgccgat atcggcatag agcgaggcca
     1981 tgtcggtgag cacgccgatc ttgacgggcg cggtctgcgc cgccgcgccg gcggcgccga
     2041 gcgccaccgc cgctaccgcc gtccatagga ttttgagcgt catccggaac ctccattctc
     2101 caaggcgtct cagactccga gatattcctg caacttctcg atattgtcgt tgagccggtc
     2161 gttggcgatg acgtcgacga cgcggccatg ctccacgaca taatggcggt cggcgacggt
     2221 ggcggcaaag cgaaagttct gctccaccag gaggatggtg aagccctttg tcttgagcgt
     2281 gcgaatgagg gcgccgatct gctgcacgat gacgggcgcc aggccctcgg tcggctcgtc
     2341 gagcagcagg agcctcgcgc cggtgcgcag gatgcggccg atcgccagca tctgctgctc
     2401 gccgcccgaa agcttggtgc cctggctctt ccggcgctcg ctcagattgg gaaagatcgc
     2461 atagatctcg tcgacgctca tgccgccggg ctgcacgatg ggcggcagca gcagattctc
     2521 ctcgacgctc aagctcgcga agatgccgcg ctcctcgggg caataggcga tgccggcgcg
     2581 ggcgatgcgg ttcgacggca ggtgaatgag ctcctgaccg ttgaagcgca cggcgccgcg
     2641 ccgcttgggc aggatgccca tcaccgcgcg caaggtggtg gtcttgccga cgccgttccg
     2701 tcccaacagc gtcaccacct ggccttgctc gacggcgaag ctcatgccgt gcagcacgtg
     2761 ggattcgcca taccaggcct cgagcccctc gacgcgcaac atctccggcg gcggcgcggc
     2821 ggcgtcaggc atggcccgtg cccatatagg cctcgcgcac ttcggcattg gccgagacct
     2881 cggcgtagct tccttcggcc aggacatggc cgcgcgtcag gaccgtgatc ttgtgggaga
     2941 gatcggcgac gaccgagaga ttgtgctcga ccatcagcac ggtgcgctgg cgcgcgacgt
     3001 ccttgatgag gtcggagatg cggccgatat cctcgtgccc cagccctgcc atgggctcat
     3061 cgaggagcag catctcgggg tcgagcgcga gcgtggtcgc gatctcgagc gcgcgcttcc
     3121 ggccgtaagg cagctccgtt gcgggactct cggcgtcgtc ggcgaggccg accgcggtca
     3181 tcagcgcgtc agcgcgcgga tcgagtgcgc ggaggctttt cgccgagcgc cagaagtgga
     3241 aggagatgcc gagcggccgc tgcagcgcga cccgcagatt ctcgcgcacc gtcagatgcg
     3301 ggaacaccgc cgagatctga aaggagcgga cca
//
LOCUS       DASYNS010000344         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1956448, whole genome shotgun sequence.
ACCESSION   DASYNS010000344 DASYNS010000000
VERSION     DASYNS010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956448"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /gene="boxB"
                     /locus_tag="VGP50_10740"
     CDS             <1..1161
                     /gene="boxB"
                     /locus_tag="VGP50_10740"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEV8027898.1"
                     /translation="GVGDEYGKPVWQQVPGEHRSSLRRLIVTQGDTEPASVEQQRLLG
                     HSCPSLYDLRNLFQVNVEEGRHLWAMVYLLHAYFGRDGREEAEELLSRHSGDADKPRI
                     LGTFNEPISDWLSFFMFTYFTDRDGKFQLKSLAESGFDPLARSCQFMLTEEAHHMFVG
                     DTGISRVVRRTLEVMKELGSEDPEAVRAKGAVDLPTIQRYLNFWFSSSLDLFGAEISS
                     NAASYFATGIKGRPDEANFADHLCKEGAMALDVPDGKGGVTSDSVPLRNAMNEAVRSA
                     YVRDCEIGVKRWNRLIVKAGYSCELTLPSPRFRRGIGAWAGQPVDPAGHVIGAAEWQA
                     KLPGWIPSDTDRAFVKNLMQRVTEPGKMAAWIAPPERGINNLPVEYEYVHLH"
     gene            1365..>2492
                     /locus_tag="VGP50_10745"
     CDS             1365..>2492
                     /locus_tag="VGP50_10745"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV8027899.1"
                     /translation="MSRFIIIGAGPAGMRAAEMLRSQSAEAEIILIGDEPYPPYDRPP
                     LSKAFITNGVAPASLSLKPESFYAEQRIELKLGHAALAIDRGAKRVALAGGAHVAYDK
                     LLLATGCGSRRLPPSLAPPSVHYLRSLEDAARIRAALAPGISLVLIGGGFIGLELAAS
                     ATKLGVEVTVLEAMPRLLTRGLPAPVCDFVHRLHESHGVRFELDARLRSIAGEAGDFV
                     VSTERQTYRCGIVVAGIGSVPHTRLAEAAGLAVEDGIRVDASGRTGDADIFAAGDATR
                     HDNPLLGRAIRVESWQVALNQAAVVARAMLGGSDVYAEMPWLWTDQYDCNIQVLGCFD
                     PSLELLLRGDAASGAFTLLGLGAGGRITLAVTVNNGRDMAVL"
BASE COUNT          401 a          857 c          820 g          414 t
ORIGIN      
        1 ggcgtcggcg acgagtacgg caagccggtc tggcagcaag tgccgggcga gcatcgctcg
       61 agcttgcgcc gcctcatcgt cacccaaggc gataccgagc ccgcttcggt cgagcagcag
      121 cgcctgctcg gccattcctg cccgtcgctc tacgacctgc gcaacctgtt ccaggtgaat
      181 gtggaggagg ggcggcacct ctgggcgatg gtctatctgc tgcacgccta tttcggccgc
      241 gacgggcgcg aggaggcgga ggagctgctg tcgcgccatt ccggcgatgc cgacaagccg
      301 cgcatcctcg gcaccttcaa cgagccgatc agcgactggc tctccttctt catgttcacg
      361 tatttcaccg accgcgacgg caagttccag ctgaagagcc tcgccgagag cggtttcgat
      421 ccactcgcgc gcagctgcca gttcatgctg accgaggaag cgcatcacat gttcgtcggc
      481 gacaccggca tctcgcgggt cgtgcgccgc acgctcgaag tgatgaagga gctcggcagc
      541 gaggatccgg aggcggtgcg cgccaagggc gcggtcgatc ttccgaccat ccagcgctat
      601 ctcaatttct ggttcagctc ctcgctcgac ctcttcggcg ccgagatctc ctcgaacgcc
      661 gccagctatt tcgcgacggg catcaagggt cggccggacg aggcgaattt cgccgaccat
      721 ctctgcaagg aaggcgcgat ggcgctcgac gtgccggacg gaaagggcgg cgtcaccagc
      781 gactccgtac cgctgcgcaa tgccatgaac gaagcggtgc gcagcgccta tgtccgcgac
      841 tgcgagatcg gcgtcaagcg ctggaaccgc ctcatcgtca aggcgggcta cagctgcgaa
      901 ttgacgctgc cgagtccgcg cttccgccgc ggcatcggcg cctgggcggg ccagcccgtc
      961 gatcccgcgg gccatgtcat cggcgccgcg gaatggcagg cgaagcttcc gggctggatc
     1021 ccgtcagaca ccgatcgcgc cttcgtcaaa aacctgatgc agcgcgtgac cgagcccggc
     1081 aagatggccg catggatcgc gccgcccgag cgcggcatca acaatctgcc ggtcgagtac
     1141 gaatacgttc atctgcattg agacttgagg acgcgcaagc gcgcttctcc ctctcccctt
     1201 gcgggagagg gaggggccca tgcgaagcat gggagggtga ggggtgaagc ggcaggtcat
     1261 cttctcagaa tgcgcagccg ggccgcaccc ctcacccggc gacgctgcgc gtcgccaccc
     1321 tctcccgcaa ggggagaggg ttggtctgcg tgtcgcctag aaccatgtcc cgcttcatca
     1381 tcatcggcgc cggcccggcg gggatgcgcg cggcagaaat gctgcgcagc caatcggccg
     1441 aagccgagat tatcctgatc ggcgacgaac cctatccgcc ctatgaccgg ccgccgctct
     1501 cgaaggcgtt catcaccaac ggcgtcgcgc cggcaagcct ctccctgaaa ccggagagct
     1561 tctatgccga gcagcggatc gaactgaagc tcggccacgc cgcgctcgcc atcgaccgcg
     1621 gcgcaaaacg cgtcgctctc gccggcggcg cacatgtcgc ttacgacaaa ctgctgctgg
     1681 cgacgggctg cggcagccgg cgattgccgc cgtccctggc gccgccttcg gtgcactacc
     1741 tgcgcagcct cgaggatgcg gcacgaatca gggcggcgct cgccccaggc atatcgctcg
     1801 ttctcatcgg cggcggcttc atcggcctcg agctcgcggc gtcggccacc aagctcggcg
     1861 tcgaggtgac ggtgctcgaa gcgatgccgc gccttttgac gcgcgggctc cccgcgccgg
     1921 tctgcgattt cgtgcatcgc ctgcatgaga gccatggcgt ccgtttcgag ctcgatgcgc
     1981 ggctcaggag catcgcgggt gaagcgggcg atttcgtcgt gagcacggag cgtcagacct
     2041 atcgctgcgg catcgtcgtt gcgggcatcg gctccgttcc ccatacgcgc ctcgccgaag
     2101 cggcgggcct tgccgtcgag gacggcattc gcgtcgacgc gagcggccgc accggcgatg
     2161 ccgacatctt cgcggccggc gatgcgacgc ggcacgacaa tccgctgctc ggccgcgcca
     2221 ttcgcgtcga atcctggcag gtcgcgctca accaggccgc tgtggtcgcg cgcgccatgc
     2281 tcggcggcag cgacgtctat gccgagatgc cctggctctg gaccgaccaa tatgattgca
     2341 acatccaggt gctcggctgc ttcgatccga gcctcgagct gctgttgcgc ggcgacgccg
     2401 cgtccggcgc cttcacgctg ctcggcttgg gcgcgggcgg gcgaatcacc cttgcggtca
     2461 cggtgaacaa cggccgcgac atggccgtgc tg
//
LOCUS       DASYNS010000345         6337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3985862, whole genome shotgun sequence.
ACCESSION   DASYNS010000345 DASYNS010000000
VERSION     DASYNS010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3985862"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="VGP50_10750"
     CDS             <1..148
                     /locus_tag="VGP50_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV8027900.1"
                     /translation="LLDHGADLRSVQEMLGHADIATTQIYTHVQGERLQRLVAEHHPL
                     ARKR"
     gene            complement(232..1128)
                     /locus_tag="VGP50_10755"
     CDS             complement(232..1128)
                     /locus_tag="VGP50_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEV8027901.1"
                     /translation="MRLASLFLLVTVLLGPLGPEARAQSDAPVPPIRFYRAESADGRV
                     TFFYPSFHLRDSRVPRPPLAMLDRVKRLVLEADIAETKAHPEVLTRYILSPKPLDLAR
                     LFTPAEIAGIRSRASCNGIAPMLESLRLSFIAVIIALPCPKADGGSYEEEVEQAARER
                     GLEVTALESAEEEFAVMAALPDRLFIAEIREYAGHLDTEDRFLERMIALYNAGDLDAL
                     YDLTMKQDMSTAADRKLFTDKILVERNRRMVGRLADVLARGDALVIIGALHFPGKDGI
                     AALLKRRGFKVTALDVENGELH"
     gene            1287..2267
                     /locus_tag="VGP50_10760"
     CDS             1287..2267
                     /locus_tag="VGP50_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEV8027902.1"
                     /translation="MSFTLIENAAPRLNRSELAVPGSQPQFFEKAAKGAADIIFLDLE
                     DAVAPDEKEKARKNIIAGLNDIDWRGKTLSLRINGLDTHYMYRDVVDVVEQAGSKLHL
                     IMIPKVGTAADVYALDMLVTQIETAKGLKRRIGFELIIETALGMQNVHAVAGASKRIE
                     SLHFGVADYAASTRARTTNIGGANPDYTVLTDKDEAGGRTLHWGDMWHYALARMVVAA
                     RANGLRPIDGPFGDFSDAEGYKAAARRAAVLGCDGKWAIHPSQVALANEVFTPSEKEV
                     TRARRILAAMEQAKREGRGAVALDGRLLDIASIRQAAALVQTAEAIAPGR"
     gene            2324..3277
                     /locus_tag="VGP50_10765"
     CDS             2324..3277
                     /locus_tag="VGP50_10765"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEV8027903.1"
                     /translation="MRHFLDFEKPLAELEGKIEELRHLSTSSEINIADEVARLEAKVD
                     RLLRQTYAKLSPWQKVQLARHPDRPHGKEYIAGLISEFTPLAGDRAFGEDRAIIGGLG
                     RFRGRSVVVIAHEKGSDTESRVRHNFGMARPEGYRKARRLMELAERFGLPVLTFVDTA
                     GAYPGIDAEERGQAEAIARSIEACLQLQVPMVASIIGEGGSGGAIALAAGNKVLMLEH
                     AVYSVITPEGCASILWRSGENAQDAAEALKLTAQDLLRLGVIDAVVPEPLGAAHRERE
                     PTIVALGDAIDAALRPLLGMDGPELRAHRREKFLAMGNRAL"
     gene            3340..4089
                     /locus_tag="VGP50_10770"
     CDS             3340..4089
                     /locus_tag="VGP50_10770"
                     /inference="COORDINATES: protein motif:HMM:NF021376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 domain-containing protein"
                     /protein_id="HEV8027904.1"
                     /translation="MTPQERDLVTSLLERLKAQGGQPKDPEADALIRRAVGEQPDAAY
                     LLAQTVIIEDMALNAAQSRIAELERQLAAATASPPAAPASFLPSAARGSVPSAGWTTS
                     RQPPTSPASSPVWSQSGATAAGPGPVAAPAAPAMAAPFASGVAGSGFLRQAATTAAGI
                     AGGALLFQGIESLFGPHYGSGGLAGLPAQPGISETVTNNYYGEPGSRQADADQDDRRD
                     SDGDAADQDVPDTDLADDGGDDLDSGDYDDV"
     gene            complement(4074..4775)
                     /locus_tag="VGP50_10775"
     CDS             complement(4074..4775)
                     /locus_tag="VGP50_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV8027905.1"
                     /translation="MRILVVEDDREAAAYLVKGLAESGHHVELAADGHDALDRASRDS
                     FDAMIIDRMLPGIDGLSIIAALRAGRDGTPVLVLSALGDVEDRVKGLRAGCDDYLPKP
                     FAFSELLARLEALTRRGNAETRLVLADLELDLLSRTVTRGGRAIDLLPREFRLLEYLM
                     RHAGHIVTRTMLLEKVWDYHFDPQTNVIDVHVSRLRQKLDKGFAVPLLHTVRGAGYTL
                     RAPPGDPLPAAQTSS"
     gene            complement(4832..>6337)
                     /locus_tag="VGP50_10780"
     CDS             complement(4832..>6337)
                     /locus_tag="VGP50_10780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEV8027906.1"
                     /translation="AAPANAQLAAPTAPVIVQLPTLAPLVKKVLPAVVNVSVSIKPGT
                     TADEDEQDQDSPGDQDQDQDQDQTGPQRNFPASPFDEFLRKFFEQQGQRFPGTPRPNL
                     GAQRVALGSGFIIDPTGYVVTNNHVVANADKVTVIFQDNSKHPAKVIGRDSKTDLALL
                     KIDTTDALPYVSWGDSNATQVGDWVLAVGNPFGLGGTVSSGIISARGRDIHSGPYDDF
                     LQIDASINRGNSGGPTFSLDGQVIGINTAIYSPNGGSVGIGFAIPSSLAKPVIEQLRE
                     HGKVERGWLGVQIQEVTPEIAKSLGLPAPEGAMVAEVNDGSPAAKGGVKQGDVILSFN
                     GHDIHKVRDLPLIVAQTPIGEKAPVKLWRDGKDVTEDVAIGQMPENPQVAKNDQGGSG
                     DPEQTSGTSVLGLKLAPLNDNVRRRADVPKNVKGVVVTGIADDSPLAALGIQPGDVIQ
                     SINQQPVTTPQQAEAKLKEAQASKNKQVLLLINRHGTSEYLALSIANNDKG"
BASE COUNT         1023 a         2102 c         2177 g         1035 t
ORIGIN      
        1 tctcctcgac catggcgcgg atctcagaag cgtgcaggag atgctgggcc atgccgatat
       61 cgcgacgacg cagatctata cccatgtgca gggggagcgc ctgcagcgtc tcgtcgccga
      121 gcatcacccg ctggcgcgga aacggtgacg cggacgcccg gataaccgct gcattcgcgc
      181 tgcgatcacg agcgccgaca gctatccggc aatgcggcgc cttggctagt gctagtgcag
      241 ctcgccgttt tcaacgtcga gcgcggtgac cttgaagccg cgccgtttca ggagagcggc
      301 gatgccatcc ttgccgggaa agtgcagagc cccgatgatc acgagggcgt cgcctcgggc
      361 caggacgtcg gcgagcctcc cgaccatcct gcgattacgt tccacgagta tcttgtcggt
      421 gaaaagcttg cggtcggccg ccgtgctcat gtcctgcttc atcgtgaggt cgtagagggc
      481 gtcgagatcg ccggcgttgt agagggcaat catccgttcg aggaatcggt cctcggtgtc
      541 gagatggccg gcatattccc tgatctccgc gatgaaaagg cggtcgggta gcgccgccat
      601 gaccgcgaac tcctcctcgg cgctctcgag cgccgtgacc tcgaggccgc gctcccgcgc
      661 ggcctgctcg acctcctcct cgtagctgcc accatcggct ttcgggcaag gcaaggcgat
      721 gatcacggcg atgaaggaga gccgcaggct ctcgagcatc ggagcgatgc cgttgcagga
      781 ggcgcgcgag cggatgccgg cgatctcggc cggcgtgaag agccgcgcca agtcgagcgg
      841 cttggggctg agaatgtagc gcgtcagcac ctccggatgc gccttcgtct cggcgatgtc
      901 ggcctcgagc accaggcgct tcacccgatc cagcatggcc aggggcgggc gcggaacgcg
      961 gctgtcgcgc aggtggaagg atggatagaa aaaggttacg cgaccgtcgg cgctttccgc
     1021 gcgatagaag cggatcggcg gcaccggcgc atccgactgc gcgcgtgctt cgggaccgag
     1081 gggtccgaga agcaccgtga ccaggagaaa caggctggct aagcgcaatt gccgctccct
     1141 aggcgttccg agagtagccc gtaacttgca tgagcgtcgt taaccggcgg ttacggtggt
     1201 gccgccggcc cccagcggat ggagcattgc acttttcgcg cggctgggaa attctccacc
     1261 gccacgccat cgccacgagg aaggtcatga gcttcactct tatcgagaac gccgctcccc
     1321 ggctgaaccg cagcgagctc gcggtgcccg gcagtcagcc gcaattcttc gagaaggcgg
     1381 ccaagggcgc cgccgacatc atttttctcg acctcgagga cgccgtggcg cccgacgaga
     1441 aggaaaaggc gcgcaagaac atcatcgccg ggctcaacga catcgattgg cgcggcaaga
     1501 ccttgtcact gcgcatcaat ggcctcgaca cgcactatat gtaccgcgac gtggtggacg
     1561 tggtcgagca ggccggaagc aagcttcact tgatcatgat cccgaaggtg gggacggcgg
     1621 ccgacgtcta tgcgctcgac atgctggtga cgcagatcga gaccgcaaag ggcttgaagc
     1681 ggcgcatcgg cttcgagctc atcatcgaga cggcgctcgg catgcagaac gtccacgccg
     1741 tcgccggcgc gtcgaagcgc atcgagtcgc tgcatttcgg cgttgccgat tacgccgcct
     1801 cgacccgcgc ccgcaccacc aacatcggcg gcgccaatcc cgactatacg gtcctcaccg
     1861 acaaggacga ggccggcggc cgcacgctcc actggggcga catgtggcac tatgcgctcg
     1921 cgcgcatggt cgtggcggcg cgcgccaatg gcttgcgccc gatcgacggc ccctttggcg
     1981 acttctccga tgccgagggc tacaaggcgg cggcgcggcg tgccgccgtg ctgggctgcg
     2041 acggcaaatg ggcgatccat ccctcgcagg tggccctcgc caacgaggtg ttcacgccgt
     2101 ccgagaagga agtgacgcgt gcccgccgta tcctcgccgc catggagcag gccaagcgcg
     2161 agggccgggg cgcggtcgcg ctcgacggcc ggctcctcga catcgcctcg atccgccagg
     2221 cggcggcgct ggtgcagacg gctgaagcga tcgcccccgg acgctgaccg ggatcgcggc
     2281 cccagtggcg ccgggcggcg cgctgtggta ggcatcgcgc ccaatgcgcc acttcctcga
     2341 tttcgagaag ccgctggccg aactcgaagg caagatcgag gagctgcggc atctctcgac
     2401 gagcagcgag atcaacattg ccgacgaggt cgcacgcctc gaggcgaagg tcgatcggct
     2461 gctgcgacag acctacgcca agctctcgcc atggcagaag gtccagctcg cgcgccatcc
     2521 cgaccggccg cacggcaagg agtatatcgc cgggctgatc agcgagttca cgccgctggc
     2581 gggcgatcgc gccttcggcg aggaccgcgc gatcatcggt gggctcggcc gcttccgcgg
     2641 ccgcagcgtc gtggtcatcg cccatgaaaa aggctcggat accgaatcgc gcgtccggca
     2701 caatttcggg atggcgcggc ccgaaggcta tcgcaaggcg cgccggctca tggagctcgc
     2761 cgagcgcttt ggcctgcccg tcttgacctt cgtcgacacc gccggagcgt atcccggaat
     2821 cgatgccgaa gagcgcggcc aggcggaagc gattgcgcgc tcgatcgagg cttgtctcca
     2881 gctccaggtg ccgatggtcg cgtcgatcat cggcgagggc ggctcgggag gcgcgatcgc
     2941 gcttgccgcc ggcaacaagg tgctcatgct ggagcatgcg gtctattcgg tgatcacccc
     3001 cgaaggctgc gcctcgatcc tgtggcgcag cggcgaaaac gcccaggacg ccgccgaggc
     3061 cctgaagctc acggcgcagg atctgctgcg gctgggcgtc atcgacgccg tcgtgccgga
     3121 gccgctgggc gccgcgcatc gcgagcgcga gccgaccatt gtcgcgctgg gcgatgccat
     3181 cgacgcggcg ctgcggccgc tcttgggcat ggacggcccc gagctccgcg ctcaccgccg
     3241 cgagaagttc ctcgccatgg gcaaccgcgc cctctgacgc atctggcaac gcgcgatcgt
     3301 cattcccatc tatgggccgg accgacgcga ggtgctgcta tgacgccgca ggaacgcgat
     3361 ctcgtcacgt cgctgctcga acgcctcaag gcacaaggcg gccagcccaa agaccccgag
     3421 gcggacgcat tgatccgccg tgccgtgggc gagcagccgg acgcggctta tcttctcgcg
     3481 cagaccgtta tcatcgagga catggccctc aatgccgccc agagccgcat cgccgagctc
     3541 gagcggcagc tcgccgcagc gacggcgtcg ccgccggccg cgccggcgag cttcctgccg
     3601 tccgcggcgc gcggcagcgt tccttcggcc ggctggacga catcgagaca gccgccgaca
     3661 tcgccggcgt catctccggt ctggagccag tctggcgcaa ccgctgcggg ccccgggcct
     3721 gtggcggctc ctgcggcgcc ggcaatggcg gcgccctttg ccagcggcgt cgcgggctcc
     3781 gggttcctgc gccaggcggc gacaacggct gccgggatcg cgggcggcgc cttgctgttc
     3841 caaggcatcg agtccttgtt cgggccgcat tacggcagcg gcgggctcgc cggcctcccg
     3901 gcgcagcccg gaatcagcga gacggtgacc aacaattact atggcgagcc gggctcgcgc
     3961 caagccgatg cggaccagga tgaccgtcgc gattcggacg gcgacgcggc cgatcaggac
     4021 gtacccgaca ccgatctcgc cgacgatggc ggcgacgatc tcgacagcgg cgattacgac
     4081 gacgtctgag ccgcgggcaa gggatcgccg ggcggcgcgc ggagcgtgta gccggcgccg
     4141 cgcacggtgt ggaggagagg cacggcgaag cccttgtcga gcttctgccg caagcggctc
     4201 acatgcacgt cgatgacgtt ggtctgggga tcgaaatgat agtcccagac cttctccagc
     4261 agcatggtgc gcgtgacgat atggccggca tgccgcatca gatattcgag caggcggaac
     4321 tcgcgcggga ggagatcgat ggcgcggccg ccgcgcgtga ccgtgcgtga gagcaggtcc
     4381 agctcgagat cggccaacac cagccgcgtt tccgcattgc cgcgccgcgt cagcgcctcg
     4441 agccgcgcca gcagctccga gaaggcaaag ggcttcggca ggtagtcgtc gcagccggca
     4501 cgcaatccct tgacccgatc ctcgacgtca ccgagcgcgc tcagcaccag gacgggcgtc
     4561 ccgtctcgcc ccgctctgag cgcggcgatg atcgacaatc cgtcgatgcc gggcagcatg
     4621 cgatcgatga tcatcgcgtc gaagctgtcg cggctggcgc gatcgagggc gtcgtgcccg
     4681 tcggcagcga gctcgacatg atggccgctc tcggccaatc ccttgacgag ataggcagcg
     4741 gcctcgcgat cgtcttcgac caccaggatg cgcatgctcg gtcccggagg atgacgaagc
     4801 gccggcgggc aaggcccgcc ggcgcgaatc gtcaaccctt gtcgttgttc gcgatcgaga
     4861 gggcgaggta ctcgctggtg ccgtggcgat tgatcagcaa cagcacctgc ttgttcttgc
     4921 tcgcctgcgc ctctttgagc ttcgcctcgg cctgctgcgg tgtcgtgacc ggctgctgat
     4981 tgatcgactg gatgacgtcg cccggctgaa tgccgagagc ggcgagcggg ctgtcatcgg
     5041 cgatgccggt gaccaccacg cccttgacgt tcttcggtac gtcggcgcgg cgacgcacat
     5101 tgtcgttgag cggtgcgagc ttcagaccga gcacggatgt gccgctcgtc tgctccggat
     5161 cgccgctgcc gccctggtcg ttcttcgcca cctgcggatt ctcgggcatc tggccgatgg
     5221 ccacgtcctc cgtgacgtcc ttgccgtcgc gccacagctt gaccggggcc ttctcgccga
     5281 tcggcgtctg cgcgacgatc agcggcaggt cacgcacctt gtggatgtcg tggccgttga
     5341 acgacaggat gacgtcgcct tgcttgacgc cgcccttggc agccgggctg ccgtcattga
     5401 cctcggccac catggcgcct tcgggcgcgg gcaggcccag gctcttggcg atctccggcg
     5461 tcacttcctg gatctggacg ccgagccagc cgcgctcgac cttgccatgc tcgcggagct
     5521 gctcgatcac cggcttggcg aggctcgagg ggatggcgaa gccgatgccg accgagccgc
     5581 cattgggcga atagatcgcc gtgttgatgc cgatcacctg gccgtcgaga ctgaaggtcg
     5641 ggccgcccga attaccgcgg ttgatcgagg cgtcaatctg caggaagtcg tcataagggc
     5701 ccgaatggat gtcgcggccg cgcgccgaga tgatgccact gctgacggtg ccgccgaggc
     5761 cgaagggatt gccgacggcc agcacccagt cgccgacctg agtggcgttg ctgtcgcccc
     5821 agctcacata aggcagcgcg tccgtggtgt cgatcttgag caaggcgaga tcggtcttgc
     5881 tgtcgcgccc gatcaccttc gcgggatgct tgctgttgtc ctggaagatg accgtcacct
     5941 tgtcggcgtt ggcgacgaca tggttgttgg tcacgacgta gccggtcgga tcgatgatga
     6001 agcccgagcc cagcgcgacg cgctgcgcgc cgaggttggg ccgcggcgtg cctgggaagc
     6061 gctggccctg ctgctcgaag aacttgcgca ggaactcgtc gaagggcgag gccgggaaat
     6121 tccgctgtgg accggtctgg tcctggtctt gatcctggtc ctggtcgccc gggctgtcct
     6181 ggtcctgctc gtcctcgtcg gcggtggtgc cgggcttgat cgacaccgat acgttgacca
     6241 cggcgggcag caccttcttc accagcggcg ccagggtcgg gagctggacg atgacgggcg
     6301 cggtcggcgc cgcaagctgg gcgtttgccg gggcggc
//
LOCUS       DASYNS010000346         8377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3986424, whole genome shotgun sequence.
ACCESSION   DASYNS010000346 DASYNS010000000
VERSION     DASYNS010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3986424"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VGP50_10785"
     CDS             complement(<1..693)
                     /locus_tag="VGP50_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV8027907.1"
                     /translation="MNFADLGLSSEVLKAVSDAGYTAPTPIQEQAIPHVLTGRDVLGC
                     AQTGTGKTAGFTLPMIDILAAGRARARMPRSLILEPTRELAAQVSNAFETYGKHHKLS
                     MALLIGGESFTDQERKLDRGVDVLIATPGRLIDLFERGKILLSDVKILVIDEADRMLD
                     MGFIPDVERIVGLLPRIRQTLFFSATMPPEIRRLADAFLMNPKEISVTAPASPAERVA
                     QHLITVDPADKRE"
     gene            846..1547
                     /locus_tag="VGP50_10790"
     CDS             846..1547
                     /locus_tag="VGP50_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV8027908.1"
                     /translation="MIRKTPLLLLPGLLCDGALWQAQMTALESVAEIEIADLTRDDSL
                     PAMAQRVLAAAPPRFALAGLSMGGYVAQEIMRQAPERVSRLALLGSSARADTPEQTAR
                     RRTLTELARKDDIVAVPRLLWPFLVHPERQDDRRLLDTLIIMAQRVGRDAFLRQEKAI
                     IARPDGRADLARIAVPSLVLCGREDHVTLLAWNEEMASLIPGAELEVIEHCGHLPTLE
                     QPEEVNAALRRWLLA"
     gene            complement(1557..2474)
                     /locus_tag="VGP50_10795"
     CDS             complement(1557..2474)
                     /locus_tag="VGP50_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3445 domain-containing protein"
                     /protein_id="HEV8027909.1"
                     /translation="MPDPTAFDDRSLRYLPFNGGQFRLAMGLMPLAAAQWIEIDGRLA
                     ADLAGKRALLETRHAEVFAALAAADAPAAELLRLLGEHLPRHHADVFRRDGQHLHNAA
                     TGETWDIEHPRLHPLDVAGRLVQEDLCLLQSTGGPYRLIAASLCAPARWRLADKIGQP
                     LTAVHEPVPGYAAALAQPVDRFFAALKAGRIVWRLNWSVFDDPAPFQPLALPSAGTIT
                     AENAGERLWLRVERQTLSRLPETGAVVFTIRTHITALASAIAAPVSAAELASLIRAMP
                     EEMRRYKVIAPIASALLAWLDARAGARSV"
     gene            2576..2983
                     /locus_tag="VGP50_10800"
     CDS             2576..2983
                     /locus_tag="VGP50_10800"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEV8027910.1"
                     /translation="MKGDIAMPEDKKKPLDCARCLAFCCKMAGYVEVSSHDIRRLAKH
                     LGLSVRDFEAKHIVKKTRAGRKRIKAGDEACQFLGEDRSCTVYAARPRDCRGYVCWDQ
                     DDNTVYDFARFTQFGVDELRKIEEKEEAEARQK"
     gene            3031..3345
                     /locus_tag="VGP50_10805"
     CDS             3031..3345
                     /locus_tag="VGP50_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEV8027911.1"
                     /translation="MMAARTKRAPAKRKYWSAEVSRKSDALDLDNRAFTWRDPQRIAR
                     SLKQSAERSRRRKARPFQSAMSMLNFYVNRAGRNLPAGRKRVLERAKDELRRLFGKEP
                     PG"
     gene            3415..3639
                     /locus_tag="VGP50_10810"
     CDS             3415..3639
                     /locus_tag="VGP50_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEV8027912.1"
                     /translation="MLALKITKIGNSSGLLLPKEALAKLRVEQGDTVYLTETPDGFRI
                     TPYDPEFERQMTLARKVMRKHRNALRKLAK"
     gene            3636..4022
                     /locus_tag="VGP50_10815"
     CDS             3636..4022
                     /locus_tag="VGP50_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV8027913.1"
                     /translation="MTEWRWLGELLVIAVHDEQLAEHGGRAGLRDLALLQSALARPLN
                     LAAYGDPDAFDLAAAYAFGIARNHPFADGNKRTAFVAAAVFLLDNGHEIEATDEAIVT
                     VMLSVSAGSTSERELAAWLRASVVQG"
     gene            4112..4960
                     /locus_tag="VGP50_10820"
     CDS             4112..4960
                     /locus_tag="VGP50_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEV8027914.1"
                     /translation="MKRAVDSSYEDRIGRVLAYVFDHLDDDLDLNRLAEIACLSPWHW
                     HRVYAAMRGETIAATVKRLRLDRAASALVQTSLPIDAIAKRSGYPNLQSFTRIFRSVY
                     GVPPATYRKSGSHTRFRPATEGSCEAMYDLSIKKVPSMTAVTIEHIGPYMEINRAFDR
                     LLGWLAPRQLLGGEFRLFGIFYDDVATVPADKLRSRAAAVISRSVAVDVPVERTEIAA
                     GDYAVLRHKGPYADMKAAYDWLYGEWLPRSGREPADGPNLEEYLNSPRETPPTELLSD
                     LYLPLR"
     gene            4973..5431
                     /locus_tag="VGP50_10825"
     CDS             4973..5431
                     /locus_tag="VGP50_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027915.1"
                     /translation="MARSMKSWADKLAATTPHEIKRMPRDLAGMKKGEIVLVPSVRLI
                     DEFIRVLPRGARISVKDMRKTLARRHGAEVTCPVYTGYHLRTVAEAACEARAAGARVD
                     EITPVWRVLDESAPTMSKLSRANAAFITRQRTRERGSDETRDARDVGTDR"
     gene            5415..5645
                     /locus_tag="VGP50_10830"
     CDS             5415..5645
                     /locus_tag="VGP50_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027916.1"
                     /translation="MERIDDLLEQLRRLPGLKEKSRGAFYVKGGAFLHFHEDPAGLFA
                     DLKENGDWVRYPVNTTVEKKRLVEQARMVLNR"
     gene            complement(5655..7640)
                     /gene="parE"
                     /locus_tag="VGP50_10835"
     CDS             complement(5655..7640)
                     /gene="parE"
                     /locus_tag="VGP50_10835"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV8027917.1"
                     /translation="MVARTPDLFDGPPKKRGESYSAKDIEVLEGLEPVRRRPGMYIGG
                     TDEGALHHLVAEVLDNAMDEAVAGHASRIELELHKDGSVVVRDNGRGIPVDPHPKFPK
                     TSALEIILTTLHSGGKFSGDAYKTSGGLHGVGVSVVNALSDRLWVEVARDKRLWTQEY
                     SRGKPKTKLKDAGAVSNRRGSTIGFHPDPQIFGEKARFKPALLYRLARSKAYLFRGVE
                     IRWWSDPELPRPEDVPQEERLHFPGGLADYLASILEGRATLTPQPFIGEHEFGKEGRV
                     EWAIAWPEDEEGFVSFYCNTVPTPLGGTHEQGLRSGLTRSLKAYGELTNNRRAQQVTA
                     DDVMTGAVVLLSLFIENPQFQGQTKERLASPEATRLVEGALKDHFDHWLSGDPEAARL
                     LIDQVIERAEERQRRRQQKDIDRKSATRKLRLPGKLADCSRGGAVGTEIFLVEGDSAG
                     GSAKQARDRETQAILPLRGKILNVASASADKLRQNQEITDLIQALGCGSGERYAEDKL
                     RYERVIIMTDADVDGAHIASLLMTFFFREMPKLVATGRLFLAAPPLYRLHQGDTTLYA
                     RDDAHKDALMKSAFNGKGKIEISRFKGLGEMPPPQLRATTMNAATRMLLRVALPSEDK
                     REFTDTVELVESLMGRKPELRYQFIQEHAAFVRDLDV"
     gene            8062..>8377
                     /locus_tag="VGP50_10840"
     CDS             8062..>8377
                     /locus_tag="VGP50_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEV8027918.1"
                     /translation="MTDAIQADAPPAIEPAIRTVAMPADANAHDDIFGGWVLSQMDLA
                     GGGVAAQRAGGRVATVAVTAMTFHRPVLVGDEVSCYGRVVKVGRTSIAVHVEVWVRRD
                     VTA"
BASE COUNT         1422 a         2703 c         2853 g         1399 t
ORIGIN      
        1 ctcgcgcttg tcggcgggat cgacggtgat gagatgctgc gccaccctct cggccggcga
       61 ggccggcgcc gtcaccgaga tttccttggg attcatcagg aaggcgtcgg cgagccgccg
      121 gatctccggc ggcatggtcg ccgagaagaa gagcgtctgg cggatccggg gcaggaggcc
      181 gacgatgcgt tcgacatcgg ggatgaagcc catgtcgagc atgcggtcgg cctcgtcgat
      241 cacgaggatc ttgacgtccg agagcaggat cttgccgcgc tcgaagagat cgataaggcg
      301 gcccggcgta gcgatcagca cgtcgacgcc gcgatcgagc ttccgctcct ggtcggtgaa
      361 gctttcaccg ccgatgagca gcgccatgct gagcttgtgg tgcttgccat aggtctcgaa
      421 agcattcgag acctgggccg cgagctcgcg cgtcggttcg aggatgagcg agcgcggcat
      481 gcgcgcacga gcgcggccgg cagcaaggat gtcgatcatc gggagggtga agccggcggt
      541 cttgcccgta ccggtttgcg cacaaccgag cacgtcgcgc ccggtaagca catgaggaat
      601 ggcctgttcc tggatcgggg tcggggcggt gtatccagcg tcggaaaccg cctttagaac
      661 ctccgaagag aggccgagat cagcaaaatt catcgagtgt gttactttgt ctttctcagt
      721 tctgactttg cgtcgccctt taattggggg tatcggctgg aaagtcaaat tatttcgcgc
      781 cgacgcgcca gagcaacagg gtgagtgtcg ccccgaatgg ttaacatctc gtagagagac
      841 cccgaatgat cagaaagacg ccactgctcc tgctccccgg tctcctctgc gatggtgcct
      901 tgtggcaggc gcaaatgacc gcgctcgaga gcgtcgcgga gatcgagatc gccgatctga
      961 cgcgcgacga ttcgctaccc gccatggcgc agcgggtcct ggccgcggcg ccgccgcgct
     1021 tcgcgctcgc cggcctgtcg atgggcggct atgtcgccca ggagatcatg cgccaggcgc
     1081 ccgaaagagt atcgcgcctc gctcttctcg ggagctcggc gcgggccgat acgccggagc
     1141 agacggcacg ccgccgcacg ctcaccgagc tcgcccgcaa ggacgatatc gtcgccgtcc
     1201 cgcggctgct ctggccgttt ctcgttcacc ccgaacgaca ggacgatcgc cgtctgctcg
     1261 atacgttgat catcatggcc cagcgcgtcg ggcgcgacgc ctttctgcgc caggagaagg
     1321 cgatcatcgc gcgtcccgac ggccgcgcgg atctcgcgcg catcgccgtg ccgtcgctgg
     1381 tgctgtgcgg acgcgaagat cacgtgacgc tgcttgcctg gaacgaggag atggcatcgc
     1441 tcattcccgg cgccgagctc gaggtgatcg agcattgcgg ccatctcccc accctggagc
     1501 agccggaaga ggtcaacgcg gcgctccggc gctggctgct cgcgtagcgc ttgggctcag
     1561 accgaacgcg cgccggcgcg cgcatcgagc caggcgagaa gcgccgacgc gatcggcgca
     1621 atgaccttgt agcggcgcat ctcttccggc atcgcgcgga tcagtgatgc gagctcggcc
     1681 gcgctcaccg gcgcggcaat agcgctcgcg agcgctgtga tatgcgtgcg gatggtaaag
     1741 accacggcac cggtctcggg cagccggctg agcgtctgcc gctcgacgcg cagccagagc
     1801 cgctctcccg cattctccgc ggtgatggtt cccgcgctcg gcagcgccag cggctgaaag
     1861 ggcgcggggt cgtcgaacac cgaccagttg agccgccaga cgatgcggcc ggccttgagc
     1921 gccgcgaaga accggtcgac cggctgagcg agcgcggcgg cgtatccggg gaccggctca
     1981 tgcaccgccg tgagcggctg gccgatcttg tcggcgagcc gccagcgcgc gggtgcgcag
     2041 agcgacgcgg cgatgagacg gtagggaccg cccgtcgact gcaacaggca gagatcctcc
     2101 tggacaagcc tgcccgcgac atcgagcgga tgaagccgcg ggtgctcgat gtcccaggtc
     2161 tcgcccgtcg ccgcgttgtg caggtgctgg ccgtcgcggc ggaacacgtc ggcatgatgg
     2221 cgcggcagat gctcgcccaa gagccgcagc agctcggctg ccggcgcatc ggccgcggcg
     2281 agcgcggcaa agacctcggc gtggcgcgtc tccaacagcg cgcgcttccc cgcgaggtcg
     2341 gcggcgagcc gaccatcgat ctcgatccat tgcgcggccg cgagcggcat gaggcccatg
     2401 gccagccgga actggccgcc gttgaaaggc aggtagcgga gggagcggtc gtcgaaggct
     2461 gtcggatcgg gcatggcggc gggtgcggat gaacggcatc gcacgcttat cattagacac
     2521 cccgcgcttc tgttgctatg gctttatcgc cgccgcagcg accgcggcga aatgcatgaa
     2581 gggcgatatc gcgatgcccg aagacaagaa gaagccgctc gattgcgcgc gctgcctggc
     2641 cttctgctgc aagatggcgg gatatgtcga ggtcagctcg catgacatcc gccggctggc
     2701 caagcatctc ggcctcagcg tgcgcgactt cgaggcgaag cacatcgtca agaagacccg
     2761 cgccggcagg aagcgcatca aggccggcga cgaggcctgc cagttcctcg gcgaggaccg
     2821 ctcctgcacc gtctatgcgg cgcgcccgcg ggattgccgc ggctatgtct gctgggacca
     2881 ggacgacaac accgtctatg atttcgcgcg cttcacgcaa ttcggcgtcg acgagttgcg
     2941 caagatcgag gaaaaggaag aggcggaggc gcggcagaag tagcgggaac ctccgtgagc
     3001 gccgtcgtgt tgggagcgac gagggctcga atgatggcag ccagaacgaa gcgcgcaccc
     3061 gccaagcgaa aatactggtc ggccgaggtg agccggaaaa gcgacgcgct cgacctcgac
     3121 aaccgcgcct tcacctggcg cgatccccag cgcattgcgc gctcgctcaa gcagtcggcg
     3181 gagcgcagcc ggcggcgcaa ggctcgccct ttccaatccg cgatgtcgat gctgaatttc
     3241 tacgtcaacc gcgccggccg caatctgccg gccgggcgca agcgcgtgct ggaacgcgcc
     3301 aaggacgagc tgcggcgtct cttcggcaag gaaccgccgg gctgacgctt gcgccgcggt
     3361 cttgacgccg ggctctttat gttataacat atgttatacc atggatgaat ggccatgctg
     3421 gcgctcaaaa tcacgaagat cggaaattcg tcgggcttgt tgttgcccaa ggaagcgctg
     3481 gcgaagctcc gcgtcgagca aggcgatacc gtctatctga cggaaacgcc ggacgggttt
     3541 cgcatcacgc cttacgatcc tgagttcgag cgccagatga cgctggcgcg caaggtcatg
     3601 agaaagcacc gcaacgcgct gcgcaagctg gcgaagtgac ggagtggcgc tggctcggcg
     3661 aactgttggt tatcgccgtc catgacgagc agctcgccga gcatggcggg cgggccggcc
     3721 tgcgggatct ggcgcttctg cagtcggccc tggcgcggcc gctcaatttg gcggcttacg
     3781 gcgatcccga cgccttcgat ctcgcggcgg cctacgcttt tggcatcgcc cgcaatcatc
     3841 ccttcgccga cggcaacaag cgcacggcat tcgtcgcagc cgcggtgttc ctgctcgaca
     3901 atggtcatga gatcgaggct accgacgagg cgatcgtcac ggtcatgctg agtgtctccg
     3961 cgggctcgac gagcgaacgg gagctggcgg cctggctccg cgcaagcgtc gtccaggggt
     4021 agaccggacg tcgcacagct tcgcaagatt tgtcaaatcg cgccgcctat aatcctccgt
     4081 cgacggcgtt gccacggaag gcgggcggaa cgtgaagcgg gcggtcgaca gcagctatga
     4141 agatcggatc ggccgtgtcc tcgcctatgt cttcgatcat ctcgacgacg acctcgatct
     4201 gaaccggctg gccgagatcg cctgcctctc gccctggcac tggcaccgcg tctacgccgc
     4261 catgcggggc gagaccatcg ccgccacggt caagcggctc cgcctcgacc gcgccgcgtc
     4321 cgcgcttgtg cagacatcgt tgcccatcga cgcgatcgcg aagcgctcgg gctatccgaa
     4381 cctgcaatcc ttcacgcgca tcttcagatc cgtctatggc gtgccgccgg cgacctaccg
     4441 caagagcggc agccacacgc gatttcgccc agccaccgaa gggagctgcg aagccatgta
     4501 tgacctcagc atcaagaaag tgccgtcgat gacggcggtc acgatcgagc acatcggtcc
     4561 ctatatggaa atcaatcgtg ccttcgatcg attgctcggc tggctggcgc cgcgacaatt
     4621 gctgggcggc gagtttcggc tcttcggcat cttctatgac gacgtggcaa ccgtgccggc
     4681 ggacaagctc cgctcgcgcg cggccgccgt catcagccga agcgtcgccg tcgacgttcc
     4741 agtcgaacgg acggagatcg ccgccggcga ctatgcggtg ctgcggcaca agggacccta
     4801 tgccgacatg aaggccgctt acgactggct ttacggcgaa tggctgccgc gctcgggccg
     4861 cgagcctgcc gacgggccca atctcgagga gtatctcaac agtccccgcg agacgccgcc
     4921 cacggaattg ctgtccgacc tgtatctgcc tttgcgctga aggagatatc ccgtggcgcg
     4981 atcgatgaag agctgggccg acaagctggc ggcgaccacc ccgcacgaga tcaagcgcat
     5041 gccgagggac ctcgccggca tgaagaaagg cgagatcgtg ctcgttccct cggtgcgact
     5101 catcgacgag ttcatccggg tgcttccgcg cggggcgcgc atcagcgtca aggacatgcg
     5161 caagacgctc gcccggcgcc atggcgccga ggtgacctgc ccggtctata ccggctatca
     5221 tctgcggacg gtggcggagg cggcgtgcga agcgcgcgcg gccggcgcgc gcgtggacga
     5281 gatcacgccg gtctggcggg tcctcgacga gagcgcgccg acgatgagca agctgtcccg
     5341 cgcaaacgcc gcgttcatca cccggcagcg cactcgggaa cgggggagcg atgaaacacg
     5401 cgacgcaagg gacgttggaa cggatcgatg atctcttgga gcagctccgg cggcttcccg
     5461 gcctcaagga gaagtcgcgc ggtgccttct acgtgaaggg tggcgcgttc ctgcatttcc
     5521 atgaggaccc ggccgggctt tttgccgatc tgaaggagaa cggcgactgg gtccgttacc
     5581 ccgtcaacac gacggtggag aagaagcggc tggtcgagca ggcccggatg gtcctgaacc
     5641 gctaggcgac agcttcacac gtcgaggtcg cggacaaagg cggcgtgctc ctggatgaac
     5701 tggtagcgca actcgggctt gcggcccatc aggctctcga ccagctcgac cgtgtccgtg
     5761 aactcgcgct tgtcttcgga cggcagcgcc acgcgcagaa gcatgcgcgt cgccgcattc
     5821 atggtggtgg cgcgaagctg cggcggcggc atctcgccca gacccttgaa gcggctgatc
     5881 tcgatcttgc ccttgccgtt gaaggcgctt ttcatcagcg cgtccttgtg cgcgtcgtcg
     5941 cgcgcgtaaa gcgtcgtgtc gccctgatgc aagcggtaga gcggcggtgc cgcgaggaag
     6001 agacgccccg tcgccaccag cttcggcatc tcgcggaaga agaacgtcat caacaacgag
     6061 gcgatatgcg cgccgtcgac atcggcatcg gtcatgatga tgacgcgctc gtagcgcagc
     6121 ttgtcctcgg catagcgctc gcccgagccg cagcctaggg cctggatgag atcggtgatc
     6181 tcctggttct ggcggagctt gtcggcgctg gcggaggcga cgttcaagat cttgccgcgc
     6241 aacggaagga ttgcctgggt ctcgcggtcg cgcgcctgct tcgccgagcc gccggcgctg
     6301 tcgccttcca ccaggaagat ctcggtgccc accgcaccgc cccgcgagca atcggcgagc
     6361 ttgccgggga ggcggagctt gcgcgtggcg ctcttgcggt cgatgtcctt ttgctggcgc
     6421 cggcgctggc gctcttcggc gcgctcgatc acctggtcga tgagaagccg cgccgcttcc
     6481 ggatcgcccg agagccaatg gtcgaaatga tccttgagcg cgccttcgac cagccgtgtc
     6541 gcctcgggcg aggcgagccg ctctttggtc tgtccctgga attgcggatt ttcgatgaag
     6601 agcgagagca gcacgacggc accggtcatg acatcgtcgg cggtgacctg ctgcgcgcgg
     6661 cggttgttgg tgagctcgcc ataggccttt aggctgcgcg tcaggcccga ccgcaagcct
     6721 tgctcatgcg tcccgcccaa cggcgtcggc acggtgttgc agtagaagga cacgaagccc
     6781 tcttcgtcct cgggccaggc gatcgcccat tcgacgcggc cttccttgcc gaactcgtgc
     6841 tcgccgatga aaggctgggg cgtcagcgtc gcgcgcccct cgaggatcga ggcgagataa
     6901 tcggcaagtc cgcccgggaa gtggagacgc tcttcctgcg gcacatcctc ggggcgcggc
     6961 agttccggat cgctccacca gcggatctcg acgccgcgga agagataggc cttggagcgg
     7021 gcgagccggt agagcagcgc cggcttgaac cgcgccttct cgccgaagat ctgcggatcg
     7081 ggatggaagc cgatggtgct gccgcggcgg ttggaaacgg cgccggcgtc cttgagcttg
     7141 gtcttgggct tgccgcggct gtattcctgc gtccagagcc gcttgtcgcg cgccacctcg
     7201 acccagagcc ggtcggagag cgcgttgacg acggagacgc cgacgccgtg caggccgccc
     7261 gaagtcttgt acgcatcgcc gctgaatttg ccgccggaat gcaaggtcgt gaggatgatc
     7321 tcgagtgccg aggtcttggg gaatttcgga tggggatcga cggggatgcc gcggccattg
     7381 tcgcgcacca cgacgctgcc gtccttgtgc agctcgagct cgatccggct cgcatggccc
     7441 gccaccgcct catccatggc gttgtcgagc acttcggcga cgaggtgatg cagcgcgcct
     7501 tcatcggtcc cgccgatata catgccggga cggcgccgca ccggctctag cccttcgagg
     7561 acctcgatgt ccttggcgga atagctctcg ccgcgcttct tcggcgggcc gtcgaacagg
     7621 tcgggcgtgc gcgctaccat cgtggagcgc caatggacat acaatgaatg cggtagagca
     7681 tccccgagca cctacaacat cttgatcgga gccgaaggac catagcgaga tttccgggtg
     7741 aaaggaacac cgcatggcgc cgcggctcac gcgacgcgaa gcgtcgttgc gacctccatc
     7801 gtcttccccc tccccccttg agggggaggg taggggtggg gggtgggggg tcgccggccg
     7861 ccgggtatcg aacaacgagc gcgcggccac cccccaccct atccctcccc ctcaaggggg
     7921 gaggggacat ttagccaaag tgggcagcgc tatgtcgcgc cgcttccgca gcactcgttg
     7981 gcgacgggtc gcttgcgccg ggccgtgctc gcgccggatc atggccgccg acgctgccgg
     8041 ccatttccga aggggaccgc gatgacggac gccattcaag ctgacgcgcc gccggcgatc
     8101 gagccggcga ttcgcacggt cgccatgcca gccgacgcca atgcccatga cgacatcttc
     8161 ggcggctggg tgctctcgca gatggacctg gcagggggtg gcgtggcggc ccaacgcgcc
     8221 ggcggtcgcg tcgcgaccgt cgccgtgacc gccatgacct tccaccggcc cgtgctggta
     8281 ggcgacgagg tgagctgcta cggcagggtg gtgaaggtcg gccgcacctc catcgcggtt
     8341 catgtcgagg tctgggtcag gcgcgacgtg acggcgc
//
LOCUS       DASYNS010000347         5392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3206938, whole genome shotgun sequence.
ACCESSION   DASYNS010000347 DASYNS010000000
VERSION     DASYNS010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3206938"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..844)
                     /locus_tag="VGP50_10845"
     CDS             complement(218..844)
                     /locus_tag="VGP50_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027919.1"
                     /translation="MILTPIDSDRLPAMLDGFTASLDGPARVLFRRIAASSAERAHGP
                     QAVTHHGDACTVLGTLGMVLKPGPPQADFSWNGQAARRDTEAYVLLHEGAHFQLASPE
                     RRRRIDFGLGPGPETGARGCAERAAMLHGLAREREEAMASLLGIFWEVELGHPALASF
                     LDQNWLEGAPRPGAAAHFAAIAAALRAGGFIDADGQPTRQLRRSPDRL"
     gene            complement(894..1931)
                     /locus_tag="VGP50_10850"
     CDS             complement(894..1931)
                     /locus_tag="VGP50_10850"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEV8027920.1"
                     /translation="MAQHSLSSVPSRIFTRKPSLAARILSASLLALSLLVTLAGLGTA
                     IYLEPAPDAVAVTPQAAQPAALPVATNSEPIPFVPTAAPASPEAPPAVATPPAADAPR
                     PADPPAEPPSAGSSAPLSPSAPPQVAALEAPAAMAPALGETGRYWVEYGVFVGERYAQ
                     RLQQRLADHGLAASLVATHAPDGRPLMRVRSVALADYVEAREVAERARQALGIGPLVH
                     RSVGEPTRTPATVPVTSSTRGDQRYYWVQFGAFPRAEQAARVKNALSDSGVDTIVATA
                     HAASGRLLFLVRSVELADRDSALALAHRGQEAANVDFLVGRSREPRHGADAAASQLAA
                     SDQAKESSPRQ"
     gene            2217..2399
                     /locus_tag="VGP50_10855"
     CDS             2217..2399
                     /locus_tag="VGP50_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027921.1"
                     /translation="MTDRNEADQPSRVMAVFQEAALSFNLPRETTLGELAEELSALGK
                     IYGGMPLYVDIRLPSA"
     gene            2596..2769
                     /locus_tag="VGP50_10860"
     CDS             2596..2769
                     /locus_tag="VGP50_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027922.1"
                     /translation="MGAHILSAAEETRSQRIRDLARRCRELSEMTALPELIKELNDIA
                     ENLDNEAEMALEE"
     gene            2820..4607
                     /locus_tag="VGP50_10865"
     CDS             2820..4607
                     /locus_tag="VGP50_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV8027923.1"
                     /translation="MKPTDVRAPEYFHKVVDCQWACPAHTPVPEYIRLIAAGRYTDAY
                     MVNWKSNVFPGILGRTCDRPCEPACRRARVEKEPVAICRLKRVAADNKDDVRALLPEI
                     PTTKNGKRIALVGAGPASLTVARDLMPLGYHCVVFDSDKQAGGMIRSQIPRFRLPESV
                     IDEEVGYIADMGIEFRPGQRIDSLKALLAEGYDAIFVGSGAPRGRDLEIPGRQEAAAN
                     IHIGIDWLSSVSFGHIDKIGKRVIVLGGGNTAMDCCRSSMRLGGEQVTVVVRSGFEEM
                     KASPWEKEDAMHEGIPILNYLVPKEFTHDKGKLTGVLFEKVAVKRDEKGRRNLVPTGE
                     PDTHIPCDDVLVAVGQENAFPWIERDIGLDFDKWGMPKIDPQTMRSTIPNVFFGGDSA
                     FGPKNIIWAVAHGHDAAVSIDKLCNGEDIQVRPPPLVNLISQKMGIHEWSYDNDITLD
                     QRYRVPLRDQAITLKDIRVEVEIGFDRKVGYAEAQRCLNCDVETVFTTSLCIECDACV
                     DICPMDCITFTANGEEAELRERLAAPAGNLTQDLYISETLRTGRIMAKDEDVCLHCGL
                     CAERCPTGAWDMQKFLLEMTPAGPACRSR"
     gene            4592..>5392
                     /locus_tag="VGP50_10870"
     CDS             4592..>5392
                     /locus_tag="VGP50_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEV8027924.1"
                     /translation="MPQPITATNDFVVKFANVNGSGSASANQLFAKSILRMGVPVASR
                     NIFPSNIQGLPTWFEVRVSEAGHLGRRGGVDLMVAMNPQTWDQDVKSIEPGGYLFYDS
                     TRPMPLAKFRDDINVVGMPLTEICNAEYTDARQRQLFKNIIYVGALSALLDMDVPAIE
                     TLIGEQFQGKDKLIKPNLHALHMGRDYAKEHLKPIGLKLKPADAVGDRIFIEGNSAAA
                     LGAVYGGATVCAWYPITPSSSLAEAFSRHCRRFRTDPVTGKSRYAILQA"
BASE COUNT          950 a         1791 c         1809 g          842 t
ORIGIN      
        1 ctttcagatg gagaaaatta tgcctgcgac tgttgcctcc cttcgcctcc cgcccgcttc
       61 gcgcgttgcg ccgcccgagc gccgtcgcgt gcagccaagc gcacgagtct cgtggctccg
      121 gcgctggttc gcgcggcgcg agccgaccgc gtatcagcgc tgtctcgccg tccacatcca
      181 ctacgcatcg ccgcgcagcg cgctgtcctg aaccggctca aagccggtcg ggcgagcggc
      241 ggagctgccg cgtcggctgc ccatcagcgt cgatgaagcc gccggcgcgg agcgccgccg
      301 cgatggccgc gaaatgcgcg gccgcacccg gccgtggcgc gccctcgagc caattctggt
      361 cgaggaagga agcgagcgcc ggatggccga gctcgacctc ccaaaagatg ccgagaagcg
      421 acgccatcgc ctcttcccgc tcgcgcgcga ggccgtggag cattgccgcc cgctcggcac
      481 agccgcgggc accggtctcg ggcccgggcc cgagaccgaa atcgatgcgc cgccgccgct
      541 cgggcgacgc gagctggaaa tgcgcgccct cgtgcagcag gacataggcc tcggtgtcgc
      601 ggcgcgccgc ctggccgttc cagctgaaat ccgcctgcgg tgggccgggc ttcagcacca
      661 tgccgagagt cccgagaacg gtacaggcgt cgccatggtg cgtgacggcc tgtggaccat
      721 gcgcgcgctc ggcgcttgac gcagcgatgc ggcggaagag gacgcgtgcc ggtccatcga
      781 ggctcgcggt gaagccgtcg agcatcgcgg gcagacgatc gctatcgatc ggtgtcagga
      841 tcatggcgcg aggtttaccg cggcgcgccg ccaaaggtcg agtcgctgcg cggtcactgc
      901 cgcggcgacg actccttcgc ttgatccgat gccgccaatt gggacgcggc ggcgtccgcg
      961 ccgtggcgcg gctcgcggct gcggccgacc aggaagtcga cattggcggc ttcctggccg
     1021 cgatgggcga gggccaaggc gctgtcgcga tcggcgagct caaccgagcg gaccaggaac
     1081 aacagccgtc cgctcgccgc gtgcgcggtc gcgacgatcg tgtcgacgcc gctgtcgctc
     1141 agcgcattct tgacgcgcgc cgcctgctcg gcgcgcggga aagcgccgaa ctgcacccag
     1201 tagtagcgct gatcgccgcg ggtcgatgac gtcaccggca ctgtcgcggg ggtccgcgtc
     1261 ggttcgccga cagagcggtg caccagcgga ccgatgccga gcgcctggcg cgctctctcg
     1321 gccacttccc gggcttcgac atagtcggcg agcgcgaccg agcgtacgcg catcaaggga
     1381 cggccatcgg gcgcatgagt cgcgacgagg ctggcggcga ggccgtgatc ggcgagcctc
     1441 tgctgcaggc gctgcgcata gcgctcaccg acgaagacac cgtattcgac ccaataacgg
     1501 ccggtctcgc cgagcgcggg cgccatcgcg gccggtgcct cgagcgccgc gacctgcggc
     1561 ggcgcgctcg gcgacagcgg cgccgagctg ccggcgctgg gcggctccgc cggcggatcg
     1621 gcggggcgcg gagcatcagc ggcgggcggc gtggcgaccg cgggcggagc ctcaggtgac
     1681 gccggagcgg cggtcggtac gaatgggatc ggttcgctgt tcgttgcgac cgggagggcg
     1741 gccggctgag ccgcttgcgg cgtcaccgct acggcatccg gtgcgggctc gagataaatg
     1801 gccgtgccga ggcccgccaa agtgacgagg agactgagcg ccagaaggga cgcgctgaga
     1861 atccgcgccg caagcgacgg cttccgcgtg aagatgcgcg atggcaccga tgacaggctg
     1921 tgttgcgcca tatttgcccc cggaaaacgc ggcgccgcgt ctctcgaccg atgaaaaatc
     1981 tatcatggcc ggtgtaaacg tcacaatgcc ggggcatgta ttatcgtcga caacgcgatg
     2041 aaaatattgt ggatatccaa ggcttcgccg ctgctcttct caagtcgcgc gagtcgcgcg
     2101 gcggggacgg catcgactcg gcttcgtaac gaaggtgtcg ccgctctgat accaaatgtt
     2161 acgggacagc aacggcgacc cggggtttac tgagggccag gaggcatgga gggacgatga
     2221 ccgacagaaa cgaagcagac cagccgagtc gcgtcatggc cgtgttccag gaagccgcgc
     2281 tgtccttcaa cttgccgcgc gagacgacgc tcggcgagct ggccgaggag ctctctgcac
     2341 tcggcaagat ctatggcggc atgccgctct acgtcgacat tcgcctgccg tcggcctagc
     2401 gaaagctcgc gactcgcggg agccggatgg cggagcgccg ccgtcctttt tggcatatct
     2461 ggtgcattcc ccagggctgt tgcggtcggc cggccgatgg ggcagattac gctgctgctc
     2521 cagccgctgc tgccgagatt gagtgaagtc gggcagctcc ggcggtcaag gcgcggcaga
     2581 gcgggagacc ggcccatggg cgcccatatc ctcagcgcgg cggaagagac gcggtcgcag
     2641 cggatcagag atttggcccg gcgctgccgg gagctgtcgg aaatgacggc gctccctgag
     2701 ctcataaaag aattgaacga catcgccgaa aaccttgata acgaagcaga aatggcgttg
     2761 gaggagtgag ccttgagacg ggtggcggcc atcccggccc gccatgaagg agaggtgatt
     2821 tgaagcccac cgacgttcgg gcgcctgaat actttcataa agtcgtcgat tgccagtggg
     2881 cctgccccgc ccataccccg gtgccggagt atatccggtt gatcgccgcg ggccggtaca
     2941 ccgacgccta tatggtcaat tggaaatcga acgtcttccc gggaatcctc gggcggacct
     3001 gcgaccggcc ctgcgagccg gcctgccgcc gcgcgcgcgt cgagaaggag ccggtggcga
     3061 tctgccgctt gaagcgcgtc gccgccgaca acaaggacga tgtccgcgcg ctcctgccgg
     3121 agattccgac gacgaagaac ggcaagcgca ttgccctcgt cggcgccggc ccggcctcgc
     3181 tcaccgtcgc ccgcgacttg atgcccctgg gctatcactg cgtcgtcttc gacagcgaca
     3241 agcaggcggg cggcatgatc cgcagccaga tcccgagatt ccgcctgccc gaatcggtga
     3301 tcgacgaaga ggtcggctac atcgccgaca tggggatcga gttccggccg ggtcaacgca
     3361 tcgacagcct gaaggcgctc ctggccgaag gctatgacgc gatcttcgtc ggcagcggcg
     3421 cgccgcgtgg ccgcgatctc gagattcccg gccgccagga agcggccgcc aacatccata
     3481 tcggcatcga ctggctgtcg agcgtatctt tcggccacat cgacaagatc ggcaagcgcg
     3541 tcatcgtgct gggcggcggc aacaccgcca tggattgctg ccgctcgtcg atgcggctcg
     3601 gcggcgagca ggtgacggtc gtcgtgcgca gcggcttcga ggagatgaag gcctcgccct
     3661 gggagaaaga ggacgcgatg catgaaggca tcccgatcct caattatctc gtgcccaagg
     3721 aattcaccca cgacaagggc aagctcacgg gcgtgctctt cgagaaggtc gcggtaaagc
     3781 gcgacgagaa ggggcggcgc aacctggtgc ccacgggcga gcccgacacc cacatcccct
     3841 gcgacgacgt gctggtggcg gtcggccagg agaacgcgtt tccctggatc gagcgcgaca
     3901 tcggcctcga cttcgacaaa tggggcatgc cgaagatcga tccccagacc atgcgttcga
     3961 ccattcccaa tgtcttcttc ggcggcgact cggcgttcgg cccgaagaac atcatctggg
     4021 cggtggccca tggccatgac gccgccgtgt cgatcgacaa gctctgcaac ggcgaggaca
     4081 tccaggtccg cccgccgcca ctcgtgaacc tcatcagcca gaagatgggc atccacgaat
     4141 ggagctatga caacgacatc acgctcgacc agcgctatcg cgtgccgctg cgcgaccagg
     4201 cgatcacgct caaggacatc agggtcgagg tcgagatcgg cttcgaccgc aaggtcggct
     4261 atgccgaggc gcagcgctgc ctcaattgcg acgtcgagac cgtgttcacg acgtcgctct
     4321 gtatcgaatg cgacgcttgc gtcgacatct gcccgatgga ctgcatcacc ttcaccgcca
     4381 atggcgaaga agcggagctg cgcgagcggc tcgccgcgcc tgccggcaac ctgacgcagg
     4441 atctctatat ctccgagacc ttgcgcaccg gccgcatcat ggcgaaggac gaggatgtat
     4501 gccttcactg cgggctgtgc gccgagcgct gcccgaccgg agcctgggac atgcagaagt
     4561 tccttctcga gatgacacca gcggggccgg catgccgcag ccgataaccg cgaccaacga
     4621 tttcgtcgtc aaattcgcca acgtcaacgg ctcgggctcg gcgagtgcca accagctctt
     4681 cgccaagtcg atcctgcgca tgggtgtgcc ggtggcgtcg cgcaacatct tcccctccaa
     4741 catccaggga ctgccgacct ggttcgaggt gcgcgtgtcg gaagcggggc atctcgggcg
     4801 ccgcggcggc gtcgacctca tggtcgcgat gaacccgcaa acctgggatc aggacgtcaa
     4861 aagcatcgag cccggcggct atctcttcta tgattcgacg cggccgatgc cgctcgcgaa
     4921 gttccgcgac gacatcaatg tcgtcggcat gcccttgacc gagatctgca acgccgaata
     4981 caccgacgcc cgccagcgtc agctcttcaa gaacatcatc tatgtgggcg cgctctcggc
     5041 gctgctcgac atggacgtcc cggccatcga gacgctgatc ggcgagcagt tccagggcaa
     5101 ggacaagctg atcaagccca atctccacgc cctccatatg ggccgcgact atgccaagga
     5161 gcatctgaag ccgatcggct tgaagctcaa gccagccgac gccgtgggcg accgcatctt
     5221 tatcgaaggc aacagcgcgg cggcgctggg tgccgtctat ggcggcgcca cggtctgcgc
     5281 ctggtatccg atcacgccgt cctcctcgct cgccgaggcg ttctcgcgcc attgccggcg
     5341 cttccgcacc gatcccgtga ccggcaagag ccgctacgcc atcctccagg cc
//
LOCUS       DASYNS010000348         8864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2738603, whole genome shotgun sequence.
ACCESSION   DASYNS010000348 DASYNS010000000
VERSION     DASYNS010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2738603"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /gene="gnd"
                     /locus_tag="VGP50_10875"
     CDS             complement(<1..987)
                     /gene="gnd"
                     /locus_tag="VGP50_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEV8027925.1"
                     /translation="MQLGMIGLGRMGANMVRRLLKGGHQCVVYNKTPQPIAELEKENA
                     IGADSPADFARRLTKPRVAWLMVPAAAVDQTIAGLLPHLERGDVIIDGGNSYYIDDIR
                     RAKELAAKGIHYVDVGTSGGVWGLERGYCMMIGGETEVVRRLDPIFATLAPGPGDVPP
                     TPGREKRGGTATQGYLHSGPNGAGHFVKMVHNGIEYGLMAAYAEGLSVLKAANIGKIA
                     GEVDAETTPLRDPEHYQYDLNLTDITEVWRRGSVIASWLLDLTAVALARDPALSGFTG
                     RVSDSGEGRWTIKAAIDEGVAAHVLSSALYERFSSRGQSDFADKLLSAMRYEF"
     gene            complement(1039..1968)
                     /locus_tag="VGP50_10880"
     CDS             complement(1039..1968)
                     /locus_tag="VGP50_10880"
                     /inference="COORDINATES: protein motif:HMM:NF021389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HEV8027926.1"
                     /translation="MPRGYEHALYVQPFDQRHTFEAKMFGWTPPLSAAQTADIAEAKR
                     VIYDGFKTALAAGVPKDKAAILVDEQFGAAILRDATADGVITACPAEKSGQDEFDFEY
                     GEDFARHIEAFHPTFCKVLVRYNPEGDRALNRRQAARLKRLSDYLADAQRSRFMFELL
                     VPAEKAQLEKLGGDNNAYQRELRPRLMAETVAELQSAGVEPDVWKIEGLDRREDCERM
                     VAVVRAGGRERVGCIILGGGESDQRVREWLATAASVKGFIGFAVGRTVFWDPVVDWRA
                     GKKTREAAVAEMARHYSEFVSIFEAARRSASGA"
     gene            complement(1968..3617)
                     /gene="pgi"
                     /locus_tag="VGP50_10885"
     CDS             complement(1968..3617)
                     /gene="pgi"
                     /locus_tag="VGP50_10885"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV8027927.1"
                     /translation="MTSSSSDAPLRRRPAWAALEAHVQKMKRVHLRQLFAEDRGRGER
                     LAVEAAGLYLDYSKNRITGETIELLLRLAQESGLRQRIDAMFNGDVINVSEKRAVLHV
                     ALRAPRGATIMEGGRNVVPDVHAVLDRMADFADRLRGGSWTGGTGKRIRNVINIGIGG
                     SDLGPVMAYEALRHYSDRSLTCRFVSNVDGTDFAEAVRDLDAAETLFIVSSKTFTTLE
                     TMTNAHSARDWLLARLGGAKDPIARHFVAVSTNAEEVAKFGIDTANMFGFWDWVGGRY
                     SMDSAIGLSTMIAIGPENFRAMLDGFHAIDEHFRTAPFERNLPVLLGLIAVWYNDFFG
                     AETQAVLPYEQYLKRFPAYLQQLTMESNGKHVTLDGTAVDYNTGPIYWGEPGTNGQHS
                     FYQLIHQGTRLIPCDFIAFAQALNPLGRHHDLLLANVFAQTEALAFGKTAEEVKKEGT
                     PDWLVPHRVFEGNRPSNTILAERLTPGALGKLVALYEHSVFTQGTIWQIDSFDQWGVE
                     LGKVLAQRIIPELESAKEPAPAHDSSTNALIRRYRKLRGSA"
     gene            complement(3641..4726)
                     /gene="tal"
                     /locus_tag="VGP50_10890"
     CDS             complement(3641..4726)
                     /gene="tal"
                     /locus_tag="VGP50_10890"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEV8027928.1"
                     /translation="MTATGKLHDLGQSLWLDNITRGLLESGTLRHYIDALSVTGLTSN
                     PTIFDQAISKSADYDDDLARAASGARSTEDLFFSVALADLKRAAELFDPVFARTDGVD
                     GWVSLEVSPLLAHDTERTVAAALDLHRRAGKRNLFIKIPGTPEGLPAIEEAIFAGIPV
                     NVTLLFSTEQYLAAADAYLKGVERRIAAGLNPAVGSVASLFISRWDVAVSGKVPSTLT
                     NRLGIAVSKRTYREYQEGLASRRFQRLANSGARAQRLLWASTGTKDPKAPDTLYVEAL
                     AAPFTVNTMPEGTLKAFAEHGGVAEPLPPDGGDAEEVLAAFGKAGIDIGALAKRLQDE
                     GAQSFVKSWTDLMERVDQKSAAIRRAS"
     gene            complement(4803..7157)
                     /locus_tag="VGP50_10895"
     CDS             complement(4803..7157)
                     /locus_tag="VGP50_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV8027929.1"
                     /translation="MPNVSSAPGGPGIRPTWTSSAKDMVTTALGVSRVWVTVGYGIIN
                     EVYAPETGMPQLRDLGFIVAGPSGWYEVKRVNNYHISLPEPYIPLPHIVHQSDQYRLV
                     LEVVPDPRRDVVLVSFRLIGEGVKLYTLLAPHLRNSGEHNNALAGDEHLAAWRHEGGA
                     VVLLSSTGFSRTSAGYVGTSDGWQDFAQNGRMTWTYGEALDGNVALLGELQENHGTLA
                     LALSVDITGARTKARSSLSEGYASIRQRFVAGWEEWGKRLEIPEAPADVLREAYLSAA
                     VLKVHQDRSYPGSIVASLSVPWGNSNDTSGGYHLVWARDCVEAGLALLAVGQVDDARS
                     MLCYLVAIQNADGSWPQNCFPDGHPFWTGIQLDEVAFPIILAAKLAEEDCLDGLSGVD
                     DMIRRAARYILHKGPVTPQDRWEENAGVSPFTLAVEIVALIAASQWFEGDEREYMLSL
                     ADCWNDRIEDWTYTTDGPLAAQFGVDGYYVRIGPDPAQCGLRGRVVVANRSGDTEPAV
                     AVVGMEYLHLVRLGLRSAQDRRIQNTSKITEALLKVETPLGIAYHRYNGDGYGEQADG
                     RPYDGNGIGRAWPLLTGERAHFELQAGHDPLRYLRMMARMTGPTGLIPEQVWDGPPLP
                     DRLLEPGKPTGSAMPLVWAHAEFLKLLCARKENRPLELLSSVENHLRGKAAGHGVWRW
                     RSETPFETLPADRDLVVEGEGPFLLHFGFDGWQDIQDRGSAPLPFGRHGVRLAKSDLA
                     RGGVLDFTRYFSDETRWEGSDHHVRVAPQERSPDELREPAEAEA"
     gene            complement(7145..8665)
                     /gene="glgA"
                     /locus_tag="VGP50_10900"
     CDS             complement(7145..8665)
                     /gene="glgA"
                     /locus_tag="VGP50_10900"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV8027930.1"
                     /translation="MSAETAKRVLFVTAEIFPMAKTGGLADVGAALPATLAELGVDIR
                     LMMPAYPQALDTAIRKRVVAERPGWQGAPPSRIISALTPDTQLPLFLLDCPPLYRRGG
                     GPYQDSDGRDWPDNAQRFALLSRAAAELALGAILPDWRADVVHGNDWHAGLVPAYLAD
                     RSGQRPATLLTIHNLAFQGVFSGETFASLGLPAPMFSPDGVEFHGQVSFLKAGIVYSD
                     GLTTVSPTYAKEILTPDAGCGLDGLLRTREADLVGILNGIDEQVWSPANDRHLPERYD
                     AKDLAGKQACKSHVRGELRLDDDERPLIAILSRLTKQKMADMVAESVDRITAQGAQLA
                     LIGNGESEIESSFVSVAHRHPGRVAVRIGYQEPIAHRLFAGADIVLAPARFEPCGLTP
                     IYGMRYGTLPVVRRTGGMADAVVPANQQTTSEGTATGFAFERATGEDMLASIAEALAT
                     YKDAGRWRRMQLQAMHRESGWMNSARQYLFLYDALVRKARSDRRSRDDLVSLQCRM"
BASE COUNT         1429 a         2936 c         2982 g         1517 t
ORIGIN      
        1 gaactcgtag cgcatggccg agagcagctt gtcggcgaaa tcggactggc cgcgcgagct
       61 gaagcgctca tacaaggccg atgagaggac atgggccgca acgccttcat cgatcgcggc
      121 cttgatggtc cagcgtccct ctcccgaatc cgagacccgc cccgtgaagc ccgacagcgc
      181 cggatcgcgc gccagcgcca ccgccgtgag gtcgagaagc cacgacgcga tgacgctgcc
      241 gcggcgccag acctcggtga tgtcggtgag gttgaggtcg tattggtaat gctcaggatc
      301 gcgcagcggc gtcgtctcgg catcgacctc gcccgcaatc ttgccgatat tcgccgcctt
      361 cagcaccgag aggccctcgg cataggcagc catgaggccg tattcgatgc cgttatgcac
      421 catcttgacg aaatggccgg cgccgttggg tccgctatgg agatagccct gcgtcgccgt
      481 gccgccgcgc ttctcgcgtc ccggcgtcgg cgggacgtcg cccggccccg gtgccagcgt
      541 cgcgaagatc ggatcgaggc ggcgcaccac ctccgtctcg ccgccgatca tcatgcaata
      601 gccgcgctcg aggccccaga caccgccgct ggtgccgaca tcgacataat ggatgccctt
      661 ggccgcgagc tccttggcgc gccggatgtc gtcgatgtaa taggaattgc cgccatcgat
      721 gatgacgtcg ccgcgctcga gatggggcag cagaccggcg atggtctgat ccaccgcggc
      781 cgcgggcacc atcagccagg cgacgcgcgg cttcgtcagg cgccgcgcga aatccgccgg
      841 cgagtccgcg ccgatggcgt tctccttctc cagctccgcg atcggctgcg gcgtcttgtt
      901 gtagacgacg cattgatggc cgcccttcag gaggcggcgc accatgttcg ctcccattcg
      961 cccaaggccg atcattccga gttgcacgag ctgatcctcc ggtcctggcc gatactccgg
     1021 cccgcttcgt tcaagccgtc acgcgccgga cgccgagcgt cgcgccgcct cgaagatgct
     1081 gacgaactcg ctgtagtgcc gcgccatctc cgcgaccgcc gcttcccgcg tcttcttccc
     1141 ggcccgccag tccaccaccg gatcccagaa gacggtgcgg cccacggcaa agccgatgaa
     1201 gcccttcacg ctggctgccg tcgcgagcca ctcgcgcacc cgctggtcgc tctcaccgcc
     1261 cccgaggatg atgcagccga cgcgctcgcg cccacccgcg cgcaccaccg cgaccatccg
     1321 ctcgcaatcc tcgcggcggt cgaggccttc gatcttccac acatcgggct cgaccccggc
     1381 gctctgcagc tcggcgacgg tctcggccat gaggcggggc cggagctccc gttgataggc
     1441 gttgttgtcg ccgccgagct tctccagctg cgccttctcg gccggcacca gcagctcgaa
     1501 catgaagcgg ctgcgctgtg cgtcggccag atagtcggag aggcgcttca gccgcgcggc
     1561 ctgccgccgg ttcaaggcgc ggtcgccttc gggattgtag cggaccagca ctttgcagaa
     1621 cgtcggatgg aacgcttcga tgtggcgcgc gaaatcctcg ccatactcga agtcgaattc
     1681 gtcctgtccg cttttctcgg ccgggcaggc cgtgatcacg ccgtcggccg tggcatcgcg
     1741 aaggatcgcg gcgccgaact gctcgtccac caggatcgcg gccttgtcct tgggcacgcc
     1801 cgcggcgagc gccgttttga agccgtcata gatgacgcgc ttggcttcgg cgatgtccgc
     1861 ggtctgcgcc gcgctcagcg gcggcgtcca gccgaacatc ttcgcctcga aggtgtggcg
     1921 ctggtcgaag ggctggacgt agagcgcgtg ctcatatcct ctgggcatca ggcggatcct
     1981 ctgagcttgc ggtagcgccg gatcagcgcg ttggtcgagc tgtcatgggc gggcgccggc
     2041 tccttcgcgc tctcgagctc ggggatgatg cgctgcgcca gcaccttccc cagctccacg
     2101 ccccattggt cgaaggagtc gatctgccag atcgtgccct gggtgaagac actgtgctcg
     2161 taaagcgcga ccagcttgcc cagcgccccc ggcgtcagcc tttccgcgag aatggtgttc
     2221 gaggggcggt tgccttcgaa gacgcgatgc ggcacgagcc agtcgggcgt gccttccttc
     2281 ttgacctcct ccgcggtctt gccgaacgcc agcgcctcgg tctgggcgaa gacattggcg
     2341 agcagcagat cgtgatgccg gcccagcggg ttcaaggcct gggcgaacgc aatgaagtcg
     2401 cacgggatga gccgcgtccc ctgatggatg agctgataga aggaatgctg gccgttggtc
     2461 ccgggctcgc cccaatagat ggggccggtg ttgtagtcga ccgcggtgcc gtcgagcgtg
     2521 acgtgcttgc cgttgctctc catggtgagc tgctggagat acgcgggaaa gcgcttcaga
     2581 tactgctcat agggcagcac ggcttgcgtc tcggcgccga agaaatcgtt gtaccagacg
     2641 gcgatgaggc ccagcagcac cggcaggttg cgctcgaagg gcgcggtgcg gaaatgctcg
     2701 tcgatggcgt ggaagccgtc gagcatggcg cggaaatttt cggggccgat ggcgatcatg
     2761 gtcgagaggc cgatcgctga atccatcgaa tagcgcccgc ctacccaatc ccagaagccg
     2821 aacatgttgg cggtgtcgat gccgaacttc gccacttcct cggcattggt ggagacggcg
     2881 acgaaatgcc gggcgatggg atctttggcg ccgccgaggc gggcgagcag ccagtcgcgc
     2941 gcgctatgcg cgttggtcat ggtctcgagc gtcgtgaagg tcttcgacga cacgatgaag
     3001 agcgtctccg ccgcatcgag gtcgcgcacg gcctcggcga aatcggtgcc gtcgacattg
     3061 gagacgaagc ggcaggtgag ggaccggtcg ctgtagtggc gcagcgcctc ataagccatg
     3121 accggcccga gatcggagcc gccgatgccg atgttgatga cgttgcggat gcgtttgccg
     3181 gtgccgcctg tccagcttcc gccgcgcagc cggtcggcga aatcggccat ccggtcgagc
     3241 acggcatgca cgtcggggac gacgttgcgg ccgccttcca tgatggtggc gccgcgcggc
     3301 gcgcgcagcg ccacatgcag aaccgcgcgc ttctccgaga cattgatcac gtcgccgttg
     3361 aacatcgcgt cgatgcgctg ccgcagcccc gattcttgcg ccagccgcag cagaagctcg
     3421 atcgtctcac cggtgatccg gttcttcgaa tagtcgagat agagccccgc ggcctcgacc
     3481 gccagccgct cgccgcgccc gcgatcttcg gcgaagagct gccggagatg gacgcgcttc
     3541 atcttctgga catgcgcctc gagcgccgcc caggcggggc gccggcgcaa cggggcatcg
     3601 ctcgacgatg acgtcatctt ggcacctgcc tcactttgcc tcagctcgcc cggcggatcg
     3661 cggcgctctt ctgatcgacg cgctccatca ggtcggtcca ggacttgaca aaggactggg
     3721 cgccctcgtc ctggagacgc ttcgccagtg cgccgatgtc gatgcccgcc ttgccgaacg
     3781 ccgccagcac ctcttcggca tcgccgccgt cgggcggcag aggttcggcg acgccgccat
     3841 gctcggcaaa agccttgagc gtgccttccg gcatggtgtt gacggtgaag ggtgcggcga
     3901 gcgcctcgac atagagcgta tcgggcgcct tcggatcttt cgtgccggtg ctggcccaga
     3961 gcaggcgctg ggcgcgggcg ccggaattcg cgagccgctg gaagcggcgc gatgccagcc
     4021 cttcctgata ctcgcggtaa gtacgcttgc tgacggcgat gccgaggcga ttggtcagcg
     4081 tgctcggcac tttgccgctg acggcgacgt cccagcggct gatgaagagc gaggcgacgg
     4141 agccgacggc cggattgagc cccgccgcga tgcgccgctc gacgcccttc agataggcgt
     4201 ccgcggcagc gaggtattgc tcggtcgaga agagcagcgt gacgttgacg ggaatgccgg
     4261 cgaagatcgc ttcctcgatg gccggcaaac cttccggcgt gccaggaatc ttgatgaaga
     4321 gattgcgctt gccggcccgg cggtggaggt cgagcgccgc tgcaaccgtg cgttcggtgt
     4381 catgggcgag gagcggcgac acctcgagcg atacccagcc atccacgccg tcggtccgcg
     4441 cgaagaccgg gtcgaacagc tcggccgcgc gcttgaggtc cgcgagcgct accgagaaga
     4501 acagatcttc tgtcgatcgc gcgccggacg cggctctggc gaggtcgtcg tcatagtcgg
     4561 cgctcttgct gatcgcctga tcgaaaatgg tggggttgga ggtgaggccc gtcaccgaga
     4621 gtgcgtcgat gtagtgtcga agcgtgccgc tctcgagaag cccgcgggta atgttgtcga
     4681 gccagaggct ctggccgaga tcgtggagtt tgccggttgc tgtcatcgca tcacccttca
     4741 acaccttaaa cgtcagagca gcgtcgaatg ttcgttctgc gcgcgccggt cgattgccgc
     4801 cttcatgcct cggcttcggc gggttcacgc agctcgtcgg ggctgcgctc ctgcggtgcg
     4861 acccggacat ggtggtcgct cccttcccat cgcgtctcgt cggagaagta gcgggtgaaa
     4921 tccaggactc cgccgcgcgc gagatcgctc ttggccaagc gcacgccatg gcggccgaag
     4981 ggcagcggcg ccgatccgcg gtcctggatg tcctgccatc cgtcgaagcc gaaatgcaga
     5041 aggaacggcc cttcgccctc gaccacgaga tcgcgatccg cgggcaacgt ctcgaagggc
     5101 gtctcgctcc gccaacgcca tacaccatgc cctgcggcct tgccgcgcaa atgattctcg
     5161 acgctgctca gcagctcgag cggacggttt tccttgcggg cgcagagcag cttgaggaac
     5221 tcggcatggg cccagaccag gggcatcgcg cttcccgtcg gcttgcccgg ctcgagcagg
     5281 cggtcgggaa gcggcgggcc gtcccacacc tgctcgggaa tgagcccggt ggggccggtc
     5341 atccgcgcca tcatcctgag atagcgcagc ggatcatggc cggcctgcag ctcgaaatgg
     5401 gcgcgctcgc cggtcaagag cggccaggcg cggccgatcc cgttgccgtc atagggacgg
     5461 ccatcggcct gctcgccata gccgtcgccg ttgtagcgat gataggcgat gcccaacggc
     5521 gtctcgacct tgagcaaggc ttcggtgatc ttgctcgtgt tctggatccg tcgatcctgg
     5581 gcggagcgca ggccgaggcg gacgagatgg agatactcca tgccgacgac ggcgaccgca
     5641 ggctcggtgt cacccgatcg attggccacg acgacgcgtc ctctgaggcc gcactgcgcc
     5701 gggtcgggcc cgatccgcac atagtagccg tcgacgccga actgggcggc gagaggtccg
     5761 tcggtggtgt aggtccagtc ttcgatgcgg tcgttccagc agtcggcgag cgagagcatg
     5821 tactcccgct cgtcgccctc gaaccattgc gacgcggcga tgagcgcgac gatctcgacc
     5881 gcgagggtga aggggctgac gcccgcattc tcttcccagc gatcctgggg cgtgaccggg
     5941 cccttgtgca ggatgtaccg cgccgcgcgg cgaatcatgt cgtcgacgcc gctcagcccg
     6001 tccaggcaat cttcctctgc gagcttggcg gcgaggatga tgggaaaggc cacctcgtcg
     6061 agctgaatgc ccgtccagaa gggatggccg tcggggaagc agttctgcgg ccagcttccg
     6121 tccgcgttct gtatggcgac gagatagcac agcatcgagc gcgcgtcgtc gacctggccc
     6181 acggccagca gcgccagacc cgcttcgacg caatcccgcg cccacaccag atgatagccg
     6241 cccgaggtgt cgttgctgtt tccccagggc acgctcaggc tcgcgacgat cgagccggga
     6301 tagctgcgat cctgatgcac tttgagaacg gcggccgaga gataggcttc gcgcaggacg
     6361 tcggcaggag cctcggggat ctccaggcgc tttccccact cctcccatcc cgcgacgaag
     6421 cgctgccgga tagaggcata gccttcgctc aagctcgagc gcgctttggt gcgggcgccg
     6481 gtgatgtcca ccgagagggc cagcgcgaga gtgccgtggt tttcctggag ctcgcccagg
     6541 agggcgacgt tgccgtcgag cgcctcgccg taggtccagg tcatccgccc gttctgcgca
     6601 aaatcctgcc agccgtcgga ggtgccgaca tagccggcgc tcgtccgcga gaagccggtg
     6661 ctgctcagca ggacgaccgc accgccctcg tgacgccaag ccgccaggtg ctcatcgcct
     6721 gcgagagcat tgttgtgttc gccgctgttg cgaagatgcg gcgcgaggag ggtatagagc
     6781 ttgacccctt cgccgatcag gcggaacgag acgagcacca cgtcgcggcg cgggtcaggg
     6841 accacctcca gcacgagccg gtactgatcg ctctgatgca cgatgtgggg cagcggaata
     6901 tagggctcgg gcagcgagat gtggtagttg ttgacgcgct tgacctcgta ccagccggac
     6961 ggtccggcga cgatgaagcc caggtcccgc agctgcggca tgccagtctc gggcgcgtag
     7021 acctcgttga tgatgccgta gcccaccgtg acccagaccc ggctgacgcc gagcgcggtc
     7081 gtcaccatgt ccttggcgct cgacgtccag gtcgggcgaa tgccgggacc acccggcgcg
     7141 ctgctcacat tcggcattgc aggctcacga ggtcatcacg ggatcggcgg tcggaccgcg
     7201 ccttccgcac cagcgcgtca taaaggaaca ggtattggcg ggcggagttc atccaaccgc
     7261 tctcgcgatg catcgcctga agctgcatgc ggcgccagcg tccggcgtct ttgtaggtgg
     7321 cgagcgcctc ggcgatcgag gcgagcatgt cctcgccggt cgcgcgctcg aaagcaaacc
     7381 cggtagcggt gccctcgctt gtcgtctgct gattggcggg caccaccgcg tccgccatgc
     7441 cgccggtgcg gcgcacgacc ggcagcgtgc catagcgcat gccatagatc ggcgtcaggc
     7501 cgcagggctc gaagcgcgcg ggcgcgagga cgatgtcggc gccggcaaag aggcgatggg
     7561 cgatgggctc ttgatagccg atgcgcaccg ccacgcggcc cggatggcgg tgagcgacgc
     7621 tcacgaacga ggattcgatc tcgctctcgc cattgccgat gagcgcgagc tgcgcgccct
     7681 gggcggtgat cctgtcgacg ctctcggcga ccatgtcggc cattttctgc ttcgtcaggc
     7741 ggctcaagat ggcgatgagg ggacgttcgt cgtcgtcgag acgcagctcg ccgcggacat
     7801 gactcttgca ggcctgcttc ccggcgaggt ccttggcgtc gtagcgttcc ggcaaatggc
     7861 ggtcattcgc gggactccag acctgctcgt cgatgccatt gaggataccg acgagatcgg
     7921 cttcgcgcgt ccgcagcagc ccatcgagcc cgcatccggc gtctggcgtc aatatctctt
     7981 tcgcgtaggt cgggctgacc gtggtcaacc cgtcgctgta gacgatgccg gccttgagaa
     8041 aggaaacctg gccgtgaaat tcgacgccgt cgggcgagaa catcggcgcc ggcagtccca
     8101 gcgaggcgaa ggtctcgccg gaaaagactc cttggaaggc gagattgtgg atcgtcagca
     8161 gcgtcgccgg acgctgcccg cttctgtcgg cgagataggc agggaccagg cccgcatgcc
     8221 agtcattgcc atgcacgaca tcggcgcgcc agtccggcag gatcgcgccc agcgccagct
     8281 ccgccgctgc ccggctcagc agcgcgaagc gctgggcatt gtccggccag tcgcgcccgt
     8341 cgctgtcctg atacgggccg ccgccgcgcc gataaagcgg cgggcaatcc agcagaaaga
     8401 gcggcagctg cgtgtcgggt gtcagtgcgc tgatgatgcg gctcggcggc gcgccctgcc
     8461 atcccggccg ctcggcgacg acgcgtttcc ggatcgcggt gtcgagcgcc tgcggatagg
     8521 ccggcatcat cagccggatg tcgacgccga gctcggcaag cgtggccggc aatgccgcac
     8581 ccacatcggc aagcccgccg gtcttggcca tcgggaagat ttccgccgtg acgaacagaa
     8641 cgcgctttgc ggtttccgca gacatcagaa aaagcccccg acgctgttgc cagcctgtac
     8701 ccgcgcatcc gaatgactgt agggaggcta acccggcccc tgagatgccg gccatcgcac
     8761 ctgcgtaaag gtgtagcggc gctcagcatc attccgccta taccttggtt taagcccgga
     8821 cgcgcccagc cgccgctatc cgcggcacag cggcgccgcg gtgg
//
LOCUS       DASYNS010000349         5571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_86618, whole genome shotgun sequence.
ACCESSION   DASYNS010000349 DASYNS010000000
VERSION     DASYNS010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86618"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1156)
                     /locus_tag="VGP50_10905"
     CDS             complement(56..1156)
                     /locus_tag="VGP50_10905"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV8027931.1"
                     /translation="MDQKCCGGDRLPRRRLLQGGLAAAAIGAVGADTTRRASAAEGTA
                     ARAIDIHAHYFPEAYLDVLAVEGKRYGYDCEKRGDGFVIGPVHYAQKFTDLEQRLAEM
                     DEIGVAMHALSLTAPMVYFAEPEAQELLARTWNDAASAAHEAHPGRFVALATLPMGDP
                     DRALAELERAAKLPGMRGVYLGTNVAGRDLDDSRFTAIFAEIEAKGLPVFLHPLNTLA
                     SERIGKAYYLSNLIGNPLDTAIAAAHLIFGGVLDRFPRLDIALPHSGGVMPILMGRWD
                     HGRNVRPELKHLAQPPSAYLRRFTYDTVCHAEPILKFVIAQVGIERVMLGSDYCFDMG
                     DAHPVDVVDSLGLDAAQRQQVLRGTAARLLKI"
     gene            1454..2233
                     /locus_tag="VGP50_10910"
     CDS             1454..2233
                     /locus_tag="VGP50_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV8027932.1"
                     /translation="MYKCFSLAAVAALALATAAAAQGPPAPDPALVDDLVAANHILSS
                     EGVVDGFGHVSARHDKDPRYFLLARSMAPGLVTKSDILVFDLDGNAVAADGAALYLER
                     FIHSEIYKAHPEVKAVVHSHSPSVIPFGVTAVPLRPIYHMSSFLGTGVPIFEIRGAGG
                     DATDMLVRNATLGAALAKDLGHSSVILMRGHGDVVVGDTVKQVVFRAIYTEVNARLEA
                     EALRLGDGKVTFLNDQEAAASGATNAPLVGRAWDLWKAKAK"
     gene            complement(2280..3530)
                     /locus_tag="VGP50_10915"
     CDS             complement(2280..3530)
                     /locus_tag="VGP50_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HEV8027933.1"
                     /translation="MQFAALTMPGVPSGELAVELIVVGGGLTGLTLGIACAGAGVEVA
                     VIDRESPATMLAEPFDGRTTAIAHGSQQVLEAIGLWRRVAEGAEPIREIRVADGGSPL
                     FLHYDHRELGDAPLGYIVENRKLRQALMAEAASLPRLRHLAPAAVERVEREEARAVAH
                     LADGGRVTGALVAACDGARSPLRGAAGIKTIAWNYPQVSIVCTVRHALPHRGVAVEHF
                     LPGGPFAILPMTGNRSSIVWTESAALAPALLALDEADFMQELGRRFGDYLGALALEGP
                     RWSHPLSLLHAERYAAPRLALVGDSAHVIHPIAGQGFNLGIRDVAVLAELIVDARRLG
                     LDIGGALLLERYERWRRFDNVMLAAVTDGLNRLFSNALPGITLARDLGLAAVDRLPPV
                     KRFLMRHAMGVIGDLPRLVRGEPL"
     gene            complement(3568..3990)
                     /locus_tag="VGP50_10920"
     CDS             complement(3568..3990)
                     /locus_tag="VGP50_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027934.1"
                     /translation="MDISKSMVRYYLDAITVPYFKTHDAPPRNLDAYLPQLLAMKRKL
                     MVHKDEDALKLAFEHIIGNPHIDASELAGGGYALSENEIRVLIRHVWQQLWPDAAAVK
                     SGGPADVKLVPMALDDWWASRKAGAAPGKKSDAPKAAG"
     gene            4124..4747
                     /locus_tag="VGP50_10925"
     CDS             4124..4747
                     /locus_tag="VGP50_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8027935.1"
                     /translation="MGRRGYHHGNLREALIRAALALIAEKGPFGFTVAEAARAAGVSP
                     AAPYRHFQDRNDLLADVALRGFERFEATLAAAWDEGRPDPSSAFERMGKAYLGFARGE
                     PAYYAAMFEGGIPLERHPELIEAGERVFAILRHAAETLSAQLPAGKRPPALMMGLHVW
                     ALSHGIASLFARGDAAARQLPMSAEELLEAGVLVYLQGLGLSADPGA"
     gene            4810..5208
                     /locus_tag="VGP50_10930"
     CDS             4810..5208
                     /locus_tag="VGP50_10930"
                     /inference="COORDINATES: protein motif:HMM:NF022467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HEV8027936.1"
                     /translation="MGIVAARLDEIGKPAWIALMVLSFIVFWPLGLAMLAFLIGSGRM
                     ACWKHRGHWRRGHEGFHDVTNRWFAGERPSGNRAFDAYRAETLKRLEEEQREFKDFVD
                     RLRFAKDKSEFDEFLADRRRRSENPQPPQS"
BASE COUNT          911 a         1846 c         1860 g          954 t
ORIGIN      
        1 agatcctctg cgacctctgc gtaagcctcc gcgacctccg cgttcctctt tgtccttaaa
       61 tcttcagcag ccgtgcggcc gtgccgcgga gcacctgctg ccgctgtgcg gcatcgagac
      121 cgaggctgtc gacgacgtcg accggatgcg catcacccat gtcgaagcaa tagtcgctgc
      181 cgagcatgac gcgctcgatg ccgacctggg cgatgacgaa tttgaggatc ggctcggcgt
      241 ggcaaacggt gtcgtaggtg aagcggcgga gataagcgct gggcggctgc gccagatgct
      301 tcagctccgg cctgacattg cgcccatggt cccagcggcc catcaggatc ggcatgaccc
      361 cgcccgaatg cggcagcgcg atgtcgagcc tgggaaagcg atcgagcacg ccgccgaaaa
      421 tgagatgtgc cgcggcgatc gccgtgtcga gcggattgcc gatgaggttc gagaggtaat
      481 aggccttgcc gatgcgctcg ctggcgaggg tgttgagcgg gtggaggaac accggcaggc
      541 cctttgcctc gatctccgca aagatcgccg tgaaacggga atcgtcgagg tcgcggccgg
      601 cgacgttggt gccaagatag acgccgcgca tgccgggcag cttggccgcg cgctcgagct
      661 cggccaacgc gcggtcgggg tcgcccatgg gcagcgtcgc cagcgcaacg aagcggccgg
      721 gatgcgcctc atgcgcggcg ctcgccgcgt cgttccaggt gcgcgccagg agctcctgcg
      781 cctcgggctc ggcgaaatag accatgggcg ccgtgagcga gagcgcatgc atcgcgacgc
      841 cgatctcgtc catctcggcg aggcgctgct cgaggtcggt gaatttctgc gcgtagtgga
      901 cggggccgat gacgaaaccg tcgccccgct tctcgcaatc gtagccataa cgcttgccct
      961 cgaccgcgag gacatcgaga taggcctctg gaaagtaatg cgcgtgaatg tcgatggccc
     1021 gcgccgccgt gccctcggct gcggatgctc gccgagtcgt gtctgcgccc acggcgccga
     1081 tggccgcggc agcgagcccg ccctgcaaca accgccggcg cggcagccga tcgccgccgc
     1141 agcacttctg gtccatggtg tttcctccat ccctcgtgca gcctcatcgg gccgcttgcg
     1201 agagagttta gcagatgaaa aacaccaggc ggagtctatc ttccgcgttc atctttcatc
     1261 ttccgtcttt cgccggaacg aatagactga tcggcgagca gaggtgtcgc atatggggtt
     1321 caccacggag gcacggagga cacggagaag agggttcggc gcgcgaagcg cgccgtcctt
     1381 cgcttctccg tgctctccgt gcctccgtgg tcgatctttc ctatctgcgt ctcggcgagg
     1441 ggggatccgc cgcatgtaca agtgtttttc gctggccgcc gttgccgctc tcgcgctcgc
     1501 caccgccgcc gcggcgcagg ggccgcccgc gcccgatccg gcgctggtcg acgacctcgt
     1561 ggcggcgaac cacatcctct ccagcgaggg tgtggtcgac ggcttcggcc atgtgagcgc
     1621 gcgccacgac aaggatccga ggtatttcct gctggcgcgc agcatggcac ccggcctggt
     1681 gacgaagagc gacatcctcg tcttcgatct cgacggcaac gccgtcgccg cggacggcgc
     1741 cgcgctctat ctcgagcgct tcatccacag cgagatctac aaggcgcatc ccgaggtgaa
     1801 ggcggtggtg cacagccatt cgccgtcggt catccccttc ggcgtcacgg ccgttccgct
     1861 ccgccccatc tatcacatga gctccttcct cgggaccggc gtgccgatct tcgagatccg
     1921 cggcgccggt ggcgatgcga cggacatgct cgtgcgcaac gcgacgctgg gcgcggcgct
     1981 ggcgaaggat ctcggccatt cctcggtgat cctgatgcgc ggccatggcg acgtggtcgt
     2041 cggcgacacc gtgaagcagg tggtgttccg cgccatctac accgaggtca atgccagact
     2101 cgaggccgag gcgctccgcc tcggcgacgg caaggtcact ttcctcaacg accaggaggc
     2161 cgccgcctcc ggcgccacca acgcgccgct cgtcggccgc gcctgggatc tgtggaaggc
     2221 gaaggcgaag tgagttatca gttgtcagtt tctcactgaa aactgaaaac tgacgacttc
     2281 tacaacggtt cgccccgcac caatcgcggc aggtcgccga tgacgcccat agcgtggcgc
     2341 atgaggaacc gtttcaccgg cggcagccgg tcgaccgcgg cgaggccgag atcgcgcgcc
     2401 agcgtgatcc ccggcaatgc gttcgagaag aggcggttga ggccgtcggt gaccgcggca
     2461 agcatgacgt tgtcgaagcg ccgccagcgc tcatagcgct cgagcagcag cgcgccgccg
     2521 atgtcgaggc cgaggcgccg cgcatcgacg atgagctcgg cgagcacggc gacgtcgcgg
     2581 atgccgagat tgaacccctg gccggcgatc ggatgaatca catgcgctga atcgcccacg
     2641 agtgcgagac gcggcgcggc gtagcgctcg gcgtggagca gcgacagcgg atgggaccag
     2701 cgcgggcctt caagggcgag cgcgcccagg tagtcgccga agcggcggcc aagctcctgc
     2761 atgaaatccg cttcgtcgag cgccagcagc gcgggcgcca gggccgcgct ctcggtccac
     2821 acgatggacg agcggttccc ggtcatcggc aggatggcga aaggtccgcc gggcaggaag
     2881 tgctcgaccg cgacgccgcg atgcggcaac gcgtggcgca cggtgcagac gatgctgacc
     2941 tgcggataat tccaggcgat cgtcttgatg ccggcggcgc cccgcagcgg cgagcgcgcg
     3001 ccgtcgcaag ccgccaccag cgcgcccgtg acgcgtccgc catcggcgag atgcgccacg
     3061 gcgcgcgctt cctcgcgctc gacgcgctcg accgcggccg gcgccagatg ccgcagcctc
     3121 ggcaaggatg cggcctccgc catcagcgcc tgacgcagct tccggttctc gacgatatag
     3181 cccagtggcg cgtcgccgag ctcgcggtga tcgtagtgaa ggaagagcgg cgagccgcca
     3241 tcggcgacgc ggatctcgcg aatcggctcc gcgccctcgg caacgcgccg ccagagaccg
     3301 atcgcctcca gcacctgctg cgagccatgg gcgatggccg tggtgcggcc gtcgaaaggt
     3361 tcggcgagca tcgtcgccgg cgattcgcga tcgatgacgg cgacctcgac accggcgccg
     3421 gcacaggcga tgcccagcgt gagccccgtg agcccgccgc cgacgacgat gagttcgacc
     3481 gcgagctctc ctgatggcac gcccggcatg gtcagcgcgg cgaattgcat tccgcccctg
     3541 caggcccagg agaggcggag agggaattca tcccgcggcc ttgggggcgt cgctcttctt
     3601 gcccggcgca gccccggctt ttcggctcgc ccaccagtcg tcgagcgcca tcggcacgag
     3661 cttgacgtcg gccggtccgc ccgatttcac ggccgcggca tcgggccaga gctgctgcca
     3721 gacatggcgg atgaggacgc ggatctcgtt ctcggagagg gcatagccgc cgcccgccag
     3781 ctctgacgcg tcgatatgcg gattgccgat gatgtgctcg aacgccagct tcaacgcgtc
     3841 ctcgtcctta tgcaccatga gcttgcgctt catggcgagg agctgcggca gataggcatc
     3901 gagattgcgc ggcggggcgt catgcgtctt gaagtaaggg acggtgatgg catccaggta
     3961 gtagcgcacc atcgacttgc tgatgtccaa ggcgatccct ctttcagagc gggttgttca
     4021 ttcgataaca cgccggagtg gtcccgtgaa agtcctttgc atgccgcggt ttgcctcgcg
     4081 cggccgcgtc atgtaagaag ctttcacatc ggcgagggca gggatggggc gccgcggcta
     4141 tcaccatggc aatttgcgcg aagcgctgat ccgcgcggcg ctcgcgctga tcgccgaaaa
     4201 agggcccttc ggcttcaccg tcgccgaggc ggcgcgcgcc gcgggcgtca gccccgccgc
     4261 gccctatcgc catttccagg accgcaacga tctcttggcg gatgtcgcgc tgcgcggctt
     4321 cgagcgcttc gaagcgacgc tggcagccgc gtgggacgag ggccggcccg acccttcgag
     4381 cgccttcgag cggatgggca aggcttatct gggctttgcc cgcggcgagc cggcctatta
     4441 cgcggcaatg ttcgaaggcg gcatcccgct cgagcgccat cccgagctga tcgaggcggg
     4501 cgagcgcgtt ttcgccatcc tgcgccatgc cgcggagacg ctctccgccc agcttcccgc
     4561 gggaaaacgg ccgcccgcgc tgatgatggg tcttcacgtc tgggcgcttt cccacggcat
     4621 cgcttctctc ttcgcgcgcg gcgatgccgc ggcgcgacag ctgcccatgt ctgccgagga
     4681 actgctcgag gccggtgttc tcgtctatct ccaaggcctc ggcctctcgg ccgacccggg
     4741 ggcttgacag cagccgggcg ctgcccacat tatgtaaatg taagctacat ttacatccgg
     4801 agaggccgca tgggcatcgt cgctgcgaga ctggatgaga tcggcaagcc ggcttggatt
     4861 gccctgatgg ttctcagctt catcgtgttt tggccacttg ggctggcgat gctggccttt
     4921 ctcatcggga gcggacgaat ggcatgttgg aaacaccgcg ggcattggcg ccgcggccat
     4981 gagggctttc acgacgtgac gaaccgctgg ttcgccggcg agcggccgag cggcaaccgc
     5041 gccttcgacg cctatcgcgc cgagacgctg aagcgactcg aggaagagca gcgcgaattc
     5101 aaagacttcg tcgaccgcct gcgctttgcc aaggacaaat ccgagttcga tgaattcctg
     5161 gccgatcggc gccgccgcag cgaaaatccg caacccccgc agagctgaat tgattatatt
     5221 ttaggcgcca cctagctccc tgccttcgaa gcgggcatga ccgctcgcag gatctcgggt
     5281 ctccctcaag gagagttcct tggcccttct tctccgttga gggggaagcg cgatcacaac
     5341 ggcaccgata cgaactccct ctctccttgc gagagaggga gggccgaaat catctcattc
     5401 cgattctgat catcggcggc tctcccggcc ggcgcgacct tcctccctgg catgattctt
     5461 gagttcagcg acccggccgc cgggcgaatc ggcccggccg gcatgctcgt tttagcggga
     5521 ggtagctcat gcgttttgcg tctttgaaaa agcggctggt gcgtctgttg c
//
LOCUS       DASYNS010000350         4065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1334294, whole genome shotgun sequence.
ACCESSION   DASYNS010000350 DASYNS010000000
VERSION     DASYNS010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334294"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VGP50_10935"
     CDS             <1..544
                     /locus_tag="VGP50_10935"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEV8027937.1"
                     /translation="VPPMSAVDLPRVYLYVTIVGALVGIPKTLLRQRRSESEDAGTPP
                     PAASPERPSMPPPGAPFLRRIPPKLGTDLLYIATEDHYLRVTTKLGSDLILFRLSDAI
                     AELGPAAGQQVHRSYWVARDAVAAIERNGQRTSLTLVNGAEIPVSRSYLRNLRDVGWL
                     GVPTVRPLADLTSQPPAAPF"
     gene            561..1184
                     /locus_tag="VGP50_10940"
     CDS             561..1184
                     /locus_tag="VGP50_10940"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006558816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV8027938.1"
                     /translation="MSEIAPADGKPRCPWPGDIPIYVAYHDEEWGVPEYDDRALFEKL
                     VLDGFQAGLSWITILRKRDNFRRAFDGFEPAKIARYTPKRVERLMQDEGIVRNRLKIE
                     GTIVSARAWLEMMEKGPGFSNFLWEFVGGRPQINHLRSRKDVPAETELSRKISKELGR
                     RGFKFCGPTIVYAFMQAVGMVDDHLVTCFRHPDFATPPQPAARPSAR"
     gene            complement(1198..1602)
                     /locus_tag="VGP50_10945"
     CDS             complement(1198..1602)
                     /locus_tag="VGP50_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027939.1"
                     /translation="MSPLDEKQDDWQQRVARFRRFAALLGEDEATAPLRALAREMERR
                     FLERYALGARYEELKRIGDVLIAEIDVVFAWARSSLVRAPGFTAADLREESRLCREEA
                     NAALDEATRRAFAKRALDLAMLGEEITRDKDS"
     gene            1790..2659
                     /locus_tag="VGP50_10950"
     CDS             1790..2659
                     /locus_tag="VGP50_10950"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8027940.1"
                     /translation="MSSDLSDAVITGASTGIGRGAVKVLIGRGWRVFAGVRKSADAAS
                     LRQEFGDKVVPLVFDVTDGMGVRSAAAEVRKQLAGRTLKGLVNNAGMGIGGPLMHQPI
                     DEIRRVLEVNVLGPVRVAQAFLPQLGADQSLSGGPGRIINITSVGGKIAPPFLGDYAM
                     SKHALEAFSESLRRELMIYGIDVIAIGPGAVVTPIWDKAEQTDDRPYAKTDYAAALKA
                     FKQRFIAEGRKGLPPERIGEAIHHALTAPKPRARYAVLRGRFINWTLPNFLPKRVVDR
                     LIAAQFGLTRRSA"
     gene            complement(2682..2996)
                     /locus_tag="VGP50_10955"
     CDS             complement(2682..2996)
                     /locus_tag="VGP50_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027941.1"
                     /translation="MIDNDGVEAPAASDEREARRRRSERLTRIMWAYICAAITLLSFV
                     VGLGPSFIPLGFGILGCVLAGQLLRENERRHSVIAGALALGGIMIWLTYNWPTIQRHI
                     AG"
     gene            3197..>4065
                     /locus_tag="VGP50_10960"
     CDS             3197..>4065
                     /locus_tag="VGP50_10960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV8027942.1"
                     /translation="MKFGIFYELQLPRPWTAGAELELYHNALDQLALADTLGYDYAWE
                     VEHHFLEEYSHSPAPEVFLGAASQRTRRIRLGHGILQLTTSHPARVAERVAVLDLLSR
                     GRVEFGMGESASITELEPFGRSFEEKRAVWEDAVRALIPMFREGGVRYDGPYFKFPLR
                     NVVPKPVQKPHPPLWVACSQLDTIEMAGRRGMGALGFQFVSADAAHAWLHAYYNAMTK
                     RLDRLTDYPVNPNIALVSMFMCAETDEEARRRADGATFFQFALRYYGASQDRQRPPPG
                     TVNMWELYESWKR"
BASE COUNT          655 a         1381 c         1349 g          680 t
ORIGIN      
        1 cgtgccgccg atgtccgcgg tggacctccc gcgagtctat ctctacgtca ccatcgtcgg
       61 cgcccttgtc ggcattccca agacgctgct ccggcagcgc cggagcgaga gcgaggatgc
      121 cggcacgccg ccgccggccg cttctcccga gcggccgtcg atgccgccgc cgggcgcccc
      181 gttcctgcgg cggatcccgc ccaagctcgg caccgacctg ctctatatcg ccaccgagga
      241 tcactatctc agggtcacga ccaagctcgg cagcgacctg atcctgttcc gcctctccga
      301 tgcgatcgcc gagctcgggc cggcggcggg gcagcaggtg catcgctcct actgggtcgc
      361 gcgcgacgcg gtcgccgcga tcgagcgcaa cgggcagcgc acgtcgctga cgctggtcaa
      421 cggcgcggag atacccgtca gccgcagcta tctacggaat ctgcgcgacg tgggctggct
      481 cggcgtgccg acagtccggc cgctcgccga cctcacgtct cagccgccgg cggcaccctt
      541 ctgagaggcg caggtcgcga atgagtgaaa tcgctccggc agatggaaag ccgcgctgcc
      601 cctggcccgg cgacattccg atctatgtcg cttatcacga cgaggaatgg ggcgtgcccg
      661 aatacgacga ccgcgcgctc ttcgagaagt tggtgctcga cggatttcag gccgggctct
      721 cctggatcac catcctgcgc aagcgcgata attttcgccg cgccttcgat ggcttcgagc
      781 ccgcgaagat cgcgcgctac acgccgaaga gggtcgagcg gctgatgcag gacgaaggca
      841 tcgtgcgcaa ccgcctgaag atcgaaggca cgatcgtgtc ggcgcgcgcc tggctcgaga
      901 tgatggagaa ggggccgggc ttctcgaatt ttctctggga gttcgtcggc ggccgcccgc
      961 agatcaatca cctgcgcagc cgcaaggacg tgccggccga gacggagctg tcgcgcaaga
     1021 tctcgaagga gctcggccgc cgcggcttca aattctgcgg gccgacgatc gtctatgcct
     1081 tcatgcaggc ggtggggatg gtcgacgacc atctcgtgac ctgcttccgc catcccgatt
     1141 tcgcgacccc gccgcagccc gccgcaagac catccgcgcg ctgagcacga cgacgcttta
     1201 cgagtcttta tcccgcgtga tctcctcgcc cagcatcgca aggtcgaggg cgcgcttggc
     1261 gaaggcgcgc cgcgtcgcct cgtccagcgc agcgttcgcc tcctctcggc agagccgtga
     1321 ctcctcgcgt agatccgcgg cggtgaaacc gggcgcgcgc accaggctgc tgcgcgccca
     1381 cgcgaatacc acgtcgatct cggcgatgag cacgtcgccg atccgtttca gctcttcata
     1441 ccgcgcgccg agcgcgtatc gctcgaggaa gcgccgttcc atctctcgcg cgagagcgcg
     1501 caacggcgcc gtcgcctcgt cctcgcccag caacgctgcg aatctgcgaa agcgcgccac
     1561 gcgttgctgc caatcgtcct gcttctcgtc gagtggcgac atttgtcgag gccccctgct
     1621 gtgccgaaat gataggcgtt gttctccgat ctgtcatgca gttagggctt cccgcctaga
     1681 tattcggcgc ggtaacagat ttgcatgcat gcgtagtcgc acgcgccgtg tcttctgttc
     1741 gtcgcgacgc gcgcgctgga ttatccttat gaagtgaagg gagcgagata tgagcagcga
     1801 cttgagcgat gcggtgatca ccggggcgtc gaccggcatc ggccgcggcg cggtcaaggt
     1861 tctcatcggg cgcggctggc gcgttttcgc cggcgtgcgg aagtcggccg atgccgcgtc
     1921 gcttcgccag gagttcggcg acaaggtcgt gccgctggtc ttcgacgtga ccgatgggat
     1981 gggcgtgcgc tccgccgcgg cggaggtgcg caagcagctc gcggggcgga cgctcaaggg
     2041 gctggtgaac aacgccggca tgggcatcgg cgggccgctc atgcaccagc cgatcgacga
     2101 gatccggcgt gtgctcgagg tcaatgtgct gggcccggtc agagtggcgc aagcctttct
     2161 gccgcagctc ggcgccgatc agagtctctc gggcgggccc ggtcgcatca tcaacatcac
     2221 gtcggtcggc ggcaagatcg caccgccttt cctcggcgac tatgcgatgt cgaagcatgc
     2281 gctcgaggcc ttcagcgaaa gcttgcgccg cgagctgatg atctacggca tcgacgtgat
     2341 cgcgatcggt ccgggcgcgg tcgtgacgcc gatctgggac aaggccgagc agacggacga
     2401 caggccctat gccaagaccg actacgccgc cgctctcaag gccttcaagc aacgctttat
     2461 cgcggagggt cgaaagggct tgccgcccga gcgcatcggc gaggcgatcc accacgcgct
     2521 caccgcgccc aagccgcgcg cacgctatgc cgtgctgcgt ggccggttca tcaattggac
     2581 gctgcccaat tttctgccga agcgcgtcgt cgatcgtctc atcgccgctc agttcggcct
     2641 gacgcggcga tcggcctgag gcggcgcgat ctcgggcgcc gtcagccggc gatgtggcgc
     2701 tggatcgtcg gccaattgta agtgagccag atcatgatgc cgccgagcgc cagcgcgccg
     2761 gcgatcacgc tgtgccggcg ctcgttctcg cgcaggagct ggccggcgag cacgcagcct
     2821 aggatgccga agcccagcgg gatgaagctc gggcccaggc cgacgacgaa ggacaggagc
     2881 gtgatggcgg cgcagatata ggcccacatg atgcgcgtca gccgctccga gcgccgacgt
     2941 cgcgcctcgc gctcatcgct ggcggctggc gcctcgacgc cgtcattgtc gatcattcgg
     3001 agcccctttc gcgggatggc ttccggcgca ggatagcggg gctgacccct tccgcccagg
     3061 atattgatcc tgctgccgga atcagggatg tttggagcgc cgaaagaacg aaatcagcga
     3121 ccagcatcgg cgccggacgc gcaagggcgc gttcgccggc agacgccgaa ggggccggtc
     3181 gacaggagga gcgggaatga aattcgggat cttctatgag ctgcaactgc cgcgtccctg
     3241 gacggccggc gccgagctcg agctctatca caatgccctc gaccagctcg ccctggccga
     3301 taccctcggc tacgactatg cctgggaggt cgagcaccac ttcctcgagg agtattcgca
     3361 ctcgccggcc ccggaagtct ttctcggcgc cgccagccag cgcacgcggc ggatccgcct
     3421 cggccacggc atcctccagc tcaccaccag tcatcccgcg cgcgttgccg agcgcgttgc
     3481 cgtgctcgac ctcctgagcc gcggccgagt cgagttcggc atgggcgaga gcgccagcat
     3541 caccgagctc gagcccttcg ggcgcagctt cgaggagaag cgcgcggtct gggaggacgc
     3601 ggtccgcgcc ctgatcccga tgttccgcga gggtggcgtc cgctatgacg ggccctattt
     3661 caagttcccg ctgcgcaacg tcgtgccgaa gccggtgcag aagccgcacc cgccgctctg
     3721 ggtcgcctgt tcgcagctcg acacgatcga gatggcgggc cgccgcggca tgggcgcgct
     3781 gggcttccag ttcgtcagcg ccgatgcggc ccacgcctgg ttgcatgcct attacaatgc
     3841 catgacgaag cggctcgaca ggctcacgga ttatcccgtc aatccgaaca tcgcgctggt
     3901 cagcatgttc atgtgcgccg agaccgacga ggaagcgcgc cggcgcgccg acggggcgac
     3961 cttcttccag ttcgcgctgc ggtattacgg cgcctcgcag gaccgccagc gcccgccgcc
     4021 gggcacggtc aacatgtggg agctctacga gagctggaag cgcga
//
LOCUS       DASYNS010000351         7230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2115511, whole genome shotgun sequence.
ACCESSION   DASYNS010000351 DASYNS010000000
VERSION     DASYNS010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2115511"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VGP50_10965"
     CDS             <1..660
                     /locus_tag="VGP50_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEV8027943.1"
                     /translation="AETALIGFAGAPWTVATYMVEGGTSRDFAAVKGWAFADADGFGR
                     LIDRVVEATLVYLKGQVAAGAEALQLFDSWAGVLPEAAFRRWVIEPTRKLVTALREQY
                     PKLPIIGFPRGAGLMYRAYFIETGVTAVGLDTGVPPAVARKTLQGIGPVQGNLDPLLL
                     VAGGTAMEEGVAEILGALRDGPFVFNLGHGIVPETPLEHVARLVELVRRPVDAGNRAS
                     Q"
     gene            657..1703
                     /gene="hemH"
                     /locus_tag="VGP50_10970"
     CDS             657..1703
                     /gene="hemH"
                     /locus_tag="VGP50_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV8027944.1"
                     /translation="MTKTAVVLFNLGGPDSPAAVRPFLFNLFNDPAIITLPRPLRWLL
                     AQFIALRRAPIARAIYDKIGGGSPLLANTEAQAQALEAALGEGYRVFVAMRYWHPQSR
                     VAAAAVRDFAPEDVVLLPLYPQFSTTTTASSLAAWRDAATAVGLTAPSRALCCYPVEA
                     GFIEPIATAIRRVLGRWPRGARMRLLFSAHGLPEKTVAAGDPYRWQVEQTVAALRRAL
                     GDPTLDCVTCYQSRVGRLAWIGPGTDAEIRRAGAEGVGLVVVPVAFVSEHSETLVELD
                     IEYRHLAEAAGVPTYVRVPTVAAAPEFIAALARLVRDVQGRRPATPCSGDGGRLCPPS
                     FTRCPCLAAMPENA"
     gene            1700..2146
                     /gene="hemJ"
                     /locus_tag="VGP50_10975"
     CDS             1700..2146
                     /gene="hemJ"
                     /locus_tag="VGP50_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HEV8027945.1"
                     /translation="MSWLLPAYPWVKALHIVSVVAWMAGLLYLPRLFVYHASARIGSE
                     TSETFKVMERRLLRGIMNPAMIAAYAFGILLLLTPGVVDWRMGWIYVKLASVVALTLV
                     HHRLAVWCKDFAADANHRPARLFRIVNEVPTLLLLLIVVMVVVKPF"
     gene            2414..3670
                     /gene="rho"
                     /locus_tag="VGP50_10980"
     CDS             2414..3670
                     /gene="rho"
                     /locus_tag="VGP50_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEV8027946.1"
                     /translation="MNLQELKRKTPAELLNFAEQQQIENASSLRKQELMFAILKQLAD
                     NDQAIYGDGVLEILQDGFGFLRSPEANYLPGPDDIYVSPSQVRRFGLRTGDTVEGQIR
                     APKDGERYFALLKVNQINFDEPDKLRHRINFDNLTPLYPDEKLRLELDDPTKKDLTTR
                     VIDLITPLGKGQRALIVSPPRTGKTVMLQNIAHSVAANHPEVYLLVLLIDERPEEVTD
                     MARTVKGEVVSSTFDEPAVRHVQVAEMVIEKAKRLVEHKRDVVILLDSITRLARAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEE
                     FKGTGNSEIILDRKVADKRVFPAIDITKSGTRKEELLVDKAVLAKMWVLRRVLMPMGT
                     MDGLEFLIDKLKQSKNNADFFEAMNT"
     gene            complement(3882..4856)
                     /locus_tag="VGP50_10985"
     CDS             complement(3882..4856)
                     /locus_tag="VGP50_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEV8027947.1"
                     /translation="MPHAIRFHKAGGPEVLQWEDVSVGKPGPGEARVRHTAVGLNYVD
                     TYVRSGLYPAPLPSGVGNEAAGIVEEIGPGVGDVKAGDRVAYAGGGPGSYSEVRVMPA
                     DRLVVLPKGITDQQAAAMMLKGMTTQYLIRQIYKVKQGDVILFHAAAGGVGLIACQWA
                     KALGATVIGTVGSDEKATLAKAHGCDHPIVYTREDFVERVKELTKGEKVPVVYDAVGK
                     DTFMKSLDCLKPLGMMVSFGQASGPVAPIDLGIFAQKGSLFFTRPTLNTYAAKRADML
                     AMARDLFEVVESGAVKIEIHQTYPLKDAAKAHRDLQSRKTTGSTIFTV"
     gene            complement(4875..5570)
                     /locus_tag="VGP50_10990"
     CDS             complement(4875..5570)
                     /locus_tag="VGP50_10990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV8027948.1"
                     /translation="MTDVTIRATDGGSFSAYIATPKSGKGPGIVLIQEIFGVNKVMRD
                     LANGYAAQGYTVMCPDLFWRQEPGIQITDKTEVEWKRAFELFQGFNVDKGVEDLKAAL
                     SSLRRHQACSGKVGSVGYCLGGKLAYLMATRSDSDGNVGYYGVGIQDQLDEAKTIKKP
                     LLLHVAEKDQFVPPPAQAKVKETLAANNLVTIYSYPGADHAFARIGGQHYDKTAADLA
                     NQRTADFFKKHLS"
     gene            5673..7061
                     /locus_tag="VGP50_10995"
     CDS             5673..7061
                     /locus_tag="VGP50_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8027949.1"
                     /translation="MPNAAAFELTNAGPDAADGLAAPQRSWAIVTVVLAVFMSVLDSA
                     IANVALPTIARDLNASAAGSIWVVNAYQLAITVLLLPLASLGEILGYRRVYLVGLVVF
                     TIASLCCALAASLLTLTLARVLQGVGAAGIMSVNGALVRFIYPRHLLGRGIGMVAIVV
                     AVAAALGPTVAAAILAVASWHWLFAVNLPPALLALAAARALPRNRGVKRRFDLPSALL
                     NAVFFGLLIFAIDGAAHGQGAALIAGEAVGALAVGVALVLRQLPQTAPLLPVDLLRIP
                     LFALSIATSVCSFAAQMLAYVALPFYLQDMLGRTHVETGLLMTPWPLTTGAMALLAGR
                     LADRFSAGILGGIGLAVFASGLALLASLPAMPSSADIVWRMMVCGFGFGLFQTPNNRA
                     IITAAPPERSGGASGMLPTARLLGQTTGAALAALVFALFAERATTVALVVAAAVAAVA
                     AGVSFVRVIERA"
BASE COUNT         1104 a         2411 c         2361 g         1354 t
ORIGIN      
        1 gccgagacgg cgctgatcgg cttcgccggc gcgccttgga ccgtcgcgac ctacatggtc
       61 gaaggcggga ccagtcgcga ctttgccgcc gtgaagggct gggcctttgc ggatgccgac
      121 ggcttcggcc ggctcatcga ccgcgtggtc gaggcaaccc tggtgtatct caaaggccag
      181 gtcgcggccg gggccgaggc gctgcagctc ttcgacagtt gggcgggggt cctgccggaa
      241 gccgctttcc ggcgctgggt gatcgagccg acgcgaaagc tggtgacggc actcagggag
      301 caatacccga agctgccgat catcggcttt ccgcgcggcg ccgggctgat gtatcgcgcc
      361 tatttcatcg agacgggcgt gaccgccgtc ggtctcgaca ccggcgtccc gccggccgtc
      421 gcgcgcaaga cgctgcaagg catcggcccc gtgcagggca atctcgatcc gctgctgctg
      481 gtcgccggcg gcacggccat ggaggagggc gtggctgaga tcctcggggc gttgcgcgac
      541 ggtcccttcg tgttcaatct cgggcacggc atcgtgccgg agacgccgct cgagcatgtc
      601 gcccgcctcg tcgagctggt gcgccgcccc gtcgacgccg ggaaccgcgc gtcgcaatga
      661 ccaagacggc cgtggttctg ttcaatcttg gcggcccgga ctcgccggcg gccgtgcggc
      721 cctttctgtt caatctgttc aatgatcctg ccatcatcac tctgcctagg ccgctgcgtt
      781 ggcttctcgc gcagttcatc gcattgcggc gcgcgccgat cgcgcgcgcc atctatgaca
      841 agatcggcgg cggctcgccg ctgctcgcca acaccgaagc tcaggcacag gcactggagg
      901 cggcgctcgg tgaaggctat cgcgtcttcg tcgcgatgcg ctattggcat ccgcagagcc
      961 gcgtcgctgc agcggcggtc cgcgatttcg ctcccgagga cgtcgttctc ctgccgctct
     1021 atcctcaatt ctcgacgacg accacggcat cgtcgctcgc ggcctggcgc gacgccgcga
     1081 cagcggtcgg gctgaccgca ccttcccgcg cgctctgctg ctacccggtc gaagccggat
     1141 tcatcgaacc gatcgccacc gcgatccgcc gcgtgctggg gcgctggccg cgcggcgcga
     1201 ggatgcgtct cctgttttcg gcgcatggac tgcccgagaa gaccgtggct gcgggcgacc
     1261 cctatcgctg gcaggtcgag cagaccgttg cggcgctgcg gcgggcgctg ggcgacccga
     1321 cactcgattg cgtcacctgc tatcagagcc gcgtcggacg gttggcatgg atcggacccg
     1381 gcaccgacgc cgagatccgc cgcgccggtg ccgagggcgt cggcctcgtc gtcgtgccgg
     1441 tggccttcgt ctccgagcat tccgagactc tcgtcgagct cgacatcgaa tatcgacacc
     1501 ttgcggaagc agcgggtgtg ccgacatatg tgcgcgtgcc gacggtcgct gccgcgcccg
     1561 aattcatcgc cgcgctggcg cgtctcgtgc gtgatgtgca gggccggcgt ccggcaacgc
     1621 cgtgctcggg tgacggcggt cggctctgtc ctccctcctt cacgcgctgt ccttgccttg
     1681 ccgccatgcc ggagaatgca tgagctggtt gctgccggcc tatccctggg tcaaagcgct
     1741 gcacatcgtc agcgtggtgg cctggatggc cggactcctc tatctgccgc ggctcttcgt
     1801 ctatcatgcg tccgcgcgca tcggctccga gacatcggag accttcaagg tcatggagcg
     1861 ccggctgctg cgcggcatca tgaacccggc aatgatcgcg gcctatgcct tcggcattct
     1921 gctgctgctg acgccggggg tcgtcgactg gcggatgggc tggatctacg tcaagctcgc
     1981 cagcgtcgtc gcactcacgc tcgtccatca ccggcttgcc gtctggtgca aggatttcgc
     2041 ggccgacgcc aaccatcgcc cggccagact cttccggatc gtgaacgagg tgccgacgct
     2101 gctcctcctt ctcattgtcg tgatggtggt ggtgaagccg ttctgagccg cggcgagaaa
     2161 tcttgttgcg ctgcggcgca gcaatcccca tatcggcacc aagcccatcg aatggcattt
     2221 gactcgcgcg ccgcttttcg ctaaggcttg ggtcgcccga tagcggcgtc ttcaacgtct
     2281 cttcaagaaa aatccggaaa acagcgttcg gtcccgcgcc aaagacccgt tcgccattcc
     2341 ggtccggccc tgaaacgatc ggggcctccc caacttcccg cgccaaaaac ctacctccaa
     2401 ggccctcatc ccgatgaatc tccaggaact gaaacgcaag accccggcgg agctgttgaa
     2461 tttcgccgag cagcagcaga tcgagaacgc ctcatccctg cgcaagcagg agctgatgtt
     2521 cgcgatcctc aagcagctcg ccgacaatga ccaggcgatc tacggcgatg gcgtgctcga
     2581 gatcctgcag gacggtttcg gcttcctgcg ctcgccggag gccaattacc tgccgggtcc
     2641 cgatgacatt tacgtgagcc cgagccaggt gcgccgcttc ggcctgcgca ccggcgacac
     2701 ggtcgagggc cagatccgcg cgcccaagga cggcgagcgc tacttcgccc tcctcaaggt
     2761 gaaccagatc aatttcgacg agcccgacaa gctccgccac cgcatcaact tcgacaacct
     2821 gacgccgctc tatcccgacg agaagctcag gctcgagctc gacgatccaa ctaaaaagga
     2881 tctgacgacg cgcgtgatcg acctcatcac gccgctgggc aagggccagc gcgcgctcat
     2941 cgtgtcgccg ccgcgcaccg gcaagacggt gatgctgcag aacatcgcgc attcggtcgc
     3001 ggccaatcat cccgaggtct atctcctcgt gctgctcatc gacgagcggc cggaggaggt
     3061 gaccgacatg gcgcgcacgg tgaagggcga ggtggtgagc tccaccttcg acgagccggc
     3121 ggtgcgccat gtgcaagtgg cggagatggt gatcgagaag gcgaagcgcc tggtcgagca
     3181 caagcgcgac gtggtgatcc ttctcgattc gatcacgcgt ctcgcgcgcg cctacaacac
     3241 ggtggtgccg tcctcgggca aggtgctgac gggcggcgtc gacgccaatg cgctgcagcg
     3301 gccgaagcgc ttcttcggcg ccgcgcgcaa catcgaggaa ggcggctcgc tcaccatcat
     3361 cgcgacggcg ctcatcgaca ccggctcgcg catggacgaa gtgatcttcg aggagttcaa
     3421 aggcaccggc aattccgaaa tcattctcga tcgcaaggtg gcggacaagc gcgtcttccc
     3481 cgcgatcgac atcaccaagt cgggcacgcg caaagaggag ctcctcgtcg acaaggcggt
     3541 gctggcgaag atgtgggtgc tgcgccgcgt gctcatgccc atgggcacga tggacggcct
     3601 cgaattcctc atcgacaagc tcaagcagtc gaagaacaac gccgatttct tcgaggcgat
     3661 gaatacgtag cgagcgcgta gcgctcgtct acagcggcgc cagcggtttg cgcagcaaac
     3721 cgcgagagcc gcatcgcgcg gggtagctgg cactgcggcc gtgcgtcctt cgacaagctc
     3781 aggatgaggg aggctgagtc tggcattggc tctcgatgcc gtccaagccc tccctcatcc
     3841 tgagcttgtc gaaggacgca cgatcgtatt gcagcctgcc gtcacaccgt gaagatcgtc
     3901 gagcccgtgg tcttgcgcga ttgcagatcg cgatgggcct tggccgcgtc cttcagcgga
     3961 taggtctggt ggatctcgat cttgacggcg ccggattcga cgacctcgaa gagatcccgc
     4021 gccatcgcca gcatgtcggc gcgcttggcg gcataggtgt tgagcgtcgg ccgcgtgaag
     4081 aagagcgaac ccttctgcgc gaagataccg agatcgatcg gcgcgacggg cccggaagcc
     4141 tggccgaagc ttaccatcat gccaagcggc ttcaggcaat cgagcgactt catgaaggtg
     4201 tccttgccga cggcgtcata gacgaccggc actttctcgc ctttcgtcag ctccttgacg
     4261 cgctcgacga agtcctcgcg cgtgtagacg atgggatggt cgcagccatg ggccttggcg
     4321 agcgtcgcct tctcgtcgct gccgacggtg ccgatgacgg tggcacccaa agccttcgcc
     4381 cattggcacg cgatgagccc gacgccgccg gcggcggcgt ggaacaggat cacgtcgcct
     4441 tgcttcacct tgtagatctg gcgaatgaga tattgcgtgg tcatgccctt gagcatcatc
     4501 gccgccgctt gctgatcggt gatgcctttg ggcagcacca ccaggcggtc ggcgggcatc
     4561 acgcgcactt cgctgtaaga gccgggaccg ccgccggcat aagcgacgcg atcgccggcc
     4621 ttgacgtcgc cgacgccggg gccgatctcc tcgacgatgc ccgccgcctc attgccgaca
     4681 ccgctcggca gcggcgccgg atagaggccg ctgcgcacat aggtgtcgac atagttgaga
     4741 ccgaccgcgg tgtggcggac gcgcgcctcg ccggggcccg gcttaccgac ggaaacgtcc
     4801 tcccattgca ggacctcggg accgccggcc ttgtggaagc ggatggcatg gggcatgggg
     4861 cgattctcct ctcgtcagct cagatgtttc ttgaagaagt cggcggtgcg ctggttggcg
     4921 agatcggccg ccgtcttgtc gtagtgctgt ccgccgatgc gggcaaaggc gtgatcggcg
     4981 ccgggatagc tgtagatcgt gacgaggttg ttggcggcca gagtttcctt gaccttggcc
     5041 tgcgccgggg gcggcacgaa ctggtccttc tcggcgacgt ggaggagaag cggcttcttg
     5101 atggtcttgg cttcgtcgag ctgatcctgg atgccgacgc cgtagtagcc gacattgcca
     5161 tcgctgtcgc tgcgcgtcgc catgaggtag gcgagcttgc cgcccaggca gtagccgacc
     5221 gagccgacct tgccgctgca ggcctgatgc cggcgcaagc tcgacagcgc cgccttcaaa
     5281 tcctcgacgc ccttgtcgac attgaagccc tggaaaagct cgaaggccct cttccactcg
     5341 acctcggtct tgtcggtgat ctggataccc ggctcctggc gccagaaaag atcggggcac
     5401 atcacggtgt agccttgcgc cgcgtagccg ttggcgagat cgcgcatgac cttgttgacg
     5461 ccgaagatct cctggatgag cacgatgccc ggacccttgc cgctcttggg cgtcgcgatg
     5521 taggcggaaa agctgccgcc gtcggtggcg cggatcgtca cgtcggtcat ggcccctccc
     5581 gaggttgtga gaaatctgta aattagattg cccagcttag cggtggcttc ggggcttgtc
     5641 acgctggccc cgcccctcta ctcagagcgc gcatgccgaa cgccgcggct ttcgagctga
     5701 cgaatgccgg gcccgacgcg gccgacgggc tcgctgcgcc acaacgcagc tgggcgatcg
     5761 tcaccgttgt gctcgccgtt ttcatgtcgg tactcgacag cgcgattgcc aatgtcgcgc
     5821 ttccgaccat cgcgcgcgat ctgaacgcga gcgcggcggg atcgatctgg gtggtcaacg
     5881 cctatcagct cgccatcacg gtcctgctgc tgccgctcgc gtcgctcggc gagatcctgg
     5941 ggtatcggcg cgtctatctc gtggggctcg tcgtctttac catcgcgtcg ctctgctgtg
     6001 cgcttgccgc gtcactgttg acgctgacct tggcgcgtgt gctccagggc gtcggcgccg
     6061 ccggcatcat gagcgtcaac ggcgcgctgg tccggttcat ctatccgcgc catttgctgg
     6121 ggcgtggcat cggcatggtc gcgatcgtcg tcgccgtcgc cgccgcactc gggccgaccg
     6181 tggcggccgc gattcttgcg gtggcatcct ggcactggct cttcgccgtc aacctgccgc
     6241 ccgcgctgct ggcactcgcg gcggcgcgcg ccttgccgcg caatcgcggt gtgaagcggc
     6301 ggtttgatct gccgagcgcg ctgttgaatg ccgtcttctt cggcctcctc atcttcgcga
     6361 tcgatggcgc cgcgcatggc caaggcgccg ctctcatcgc cggcgaagcg gtcggcgcgc
     6421 tggccgtcgg cgtggcgctc gtgctgcggc aattgccgca gacggcgccg ctgttgcccg
     6481 tcgacctgct gcgcattccc ctcttcgcct tgtcgatcgc cacctcggtc tgctcttttg
     6541 cggcgcagat gctggcctat gtcgcgctgc ctttttattt gcaggacatg ctcggtcgca
     6601 cccatgtcga aaccgggctt ttgatgacac cctggccgct cacgacggga gcgatggcgc
     6661 tgctcgccgg ccgtctcgcg gatcgcttct cggccggcat actcggcggc atcggcctcg
     6721 cggtgttcgc gtccggcctc gcgctcctgg cgtcgctgcc ggcaatgccg agcagtgccg
     6781 acatcgtctg gcgcatgatg gtttgcggct tcggtttcgg cttgtttcag acgcccaaca
     6841 accgcgccat catcaccgcg gcgccgcccg agcgcagcgg cggtgccagc ggcatgctgc
     6901 cgaccgcacg gcttctcggc cagacgacgg gcgccgcgct cgccgcgctc gtgttcgcgc
     6961 tgttcgcgga acgagcgacg acggtcgcct tggtggtggc cgccgcggtc gctgcggttg
     7021 ccgcgggtgt cagcttcgtg cgcgtcatcg agcgcgcatg atccgccggc gcgacactag
     7081 accctatgga cattcgcaga gatgcgcatc cttcgcctct cccgcatcgc gggagaggga
     7141 gggacccatt gcgcagcaat gggagggttg agggggcgcg tcaggcaaag cgcctttgct
     7201 tgaaccttcc ctcacccggc gcgcgctccg
//
LOCUS       DASYNS010000352         4980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3051615, whole genome shotgun sequence.
ACCESSION   DASYNS010000352 DASYNS010000000
VERSION     DASYNS010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3051615"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VGP50_11000"
     CDS             <1..1059
                     /locus_tag="VGP50_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV8027950.1"
                     /translation="PPPPVTVSAPLQREVADWATFTGQFSAVDDVEIRAQVSGYLTEI
                     HFTDGQIVHKGDLLFVIDPRPFEIQLQQAQAQQQTAAAQLELANKQLSRAVELRHGDV
                     VSADTLDQRVQQQRAAQAALEQGKAAIRAAELNLEFSRITAPITGRISTHRVSVGNLV
                     SGGPSAGSTTLLTTVVSLDPIYLDFDMSENDYLAYQRFAQSPAGHGADHAIDASLSDE
                     RGWPRQGRLDFIDNQLDRGSGTMHARATVANADLFIAPGQFARLRLPISAPRPVLLVP
                     DDALTIDQSRRLVMSVAPDGTVVPKVVAIGQLSDGLRVITEGLDANDRIVINGLARAR
                     PGAKVAPQPGSIATAARG"
     gene            1069..4263
                     /locus_tag="VGP50_11005"
     CDS             1069..4263
                     /locus_tag="VGP50_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEV8027951.1"
                     /translation="MRLSHFFIDRPIFATVLSLFITILGAIAYVALPVAQYPEIAPPT
                     IQVTASYPGASADVVSETVATPLEQQINGVQDMLYMSSQATGDGKLTLTVTFALGTNL
                     DTAQVLTQNRVSVAVPRLPDVVQRLGVTVKKNSPDMLMVVHLYSPDGSRDQLYMSNYA
                     TLQVKDVLSRLDGVGDVNIFGARDYSMRIWLDPDKVASRNLTAGDVVAALRAQNVQVS
                     SGVLGQPPLPLPGAFQLNVQTLGRLIDPAQFGDIIIKSDGAGRVTRVRDIGRVELGAQ
                     DYGANGYLDARNAVPLLIYQQPGSNALATADHVRSTIQELSQHFPQGLKYDIVYDPTR
                     FIAESVREVVKTIAAAVALVVLVVILFLQTWRASIIPIVAIPISLIGTFAVLAALGFS
                     LNSLSLFGLVLAVGIVVDDAIVVVENVERNLSAGMSPREAAHRTMDEVGGALIAIALT
                     LCAVFVPATFMSGISGQFFRQFAATIAASTVISCFISLTLSPALCALLLKPHLRAADQ
                     PRRPLLARPLFAFFRGFNRAFAAVAERYGSLTARLVRMAAIVLVAYAGLIGLTAWQFG
                     RAPTGFIPEQDQGYLITVIQLPPGASLARTDAVVRKAAEIILATPGVRHAVPFAGFDG
                     ATFTNAPNAGAIFFSYGTLALGPFEERPARGVSGDAMIAQMRQRLAAIQDAFIITIKP
                     PPVRGIGTAGGFKMMVEDRQDQGSSALDAAVQDLAGAANQVPGLVGVFSLFNTRTPAV
                     YADIDRERAEKLGVSPDKVFDALQVYLGSAYVNDFNYLGRTYQVMAQADGQFRADAQD
                     IARLKTRNDAGAMVPLGSVAQFRDVTGPYRVPRYNLYPAAELQGNTAPGVSSGYAIAA
                     MEQLAAEHLPNGFSFDWTDLAFQQKQAGDTALLIFAASVVFVFLVLAAQYESWTLPLS
                     VVLIVPMCLLAAVTGLMLRSMSVDILAQIGFVVLVGLAAKNAILIVEFARQDEEAGES
                     VAEAAVHAARTRLRPILMTSFAFILGVVPLVTASGAGAEMRQSLGTAVFAGMLGVTAF
                     GLLFTPVFYTVMRRLSRTRSRGGAEAASQSGSPA"
     gene            4430..>4980
                     /locus_tag="VGP50_11010"
     CDS             4430..>4980
                     /locus_tag="VGP50_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEV8027952.1"
                     /translation="MTLKLYAMTCGHLTCQLGPMLEGGEGEVVLPIPAYLIEHPKGTA
                     LFDTGMHPDCRRDPAARVGPDVARFFRFDYRPDEDIGARLRSIGRDPARIDFIVNSHF
                     HFDHVGGNALIPNATMVVQKAEWEAGMDAELGPRLGLDRRDFDLGHRLRRIAGEHDLF
                     GDGSVVCFPTYGHTPGHQSLRLRL"
BASE COUNT          750 a         1804 c         1563 g          863 t
ORIGIN      
        1 ccaccgccgc cagtgaccgt cagcgcgccc ctccagcggg aagtcgccga ctgggcgacc
       61 ttcaccggcc agttctccgc cgtcgacgac gtcgagatcc gcgcgcaggt gagcggctac
      121 ctgaccgaga tccatttcac cgacggccag atcgtgcata agggcgacct gctcttcgtc
      181 atcgatcccc gtcccttcga gattcagctc cagcaggcgc aggcgcagca gcagacggcc
      241 gccgcgcagc tcgagctcgc caacaagcag ctgtcgcgcg ccgtcgagct gcggcacggc
      301 gacgtcgtct ccgccgacac cctcgatcag cgcgtgcagc agcaacgcgc cgcgcaggca
      361 gcgctcgagc agggcaaggc ggccatccgc gccgccgagc tcaatctcga attctcccgc
      421 attacggcgc cgatcaccgg ccgcatcagc acccatcgcg tctcggtggg caacctggtg
      481 agcggcgggc cgagtgcggg gtcgaccacg ctgctgacga ccgtcgtctc gctggacccg
      541 atttatctcg acttcgacat gagcgagaac gactacctcg cctatcagcg cttcgcccag
      601 agcccggccg gccacggcgc cgaccacgcg atcgacgcga gcctcagcga cgagcgcggc
      661 tggccgcgcc aagggcggct cgacttcatc gacaaccagc tcgatcgcgg cagcggcaca
      721 atgcatgcgc gggcgacggt ggccaatgcg gacctcttca tcgcgccggg gcagttcgcc
      781 cggctcaggc tgccgatctc agcgccgcgg ccggtactgc tggtgcccga tgacgccttg
      841 accatcgacc agtcgcgccg gctggtgatg tcggtcgcgc cggacggcac cgtcgtgccc
      901 aaggtcgtcg cgatcggcca gctcagcgac gggctgcgcg tgatcaccga agggctcgat
      961 gccaacgacc ggatcgtgat caacggcctg gcgcgggcgc ggcccggcgc caaggtggcg
     1021 ccgcagcccg gcagcatcgc caccgccgcg cgcggctagg actctcccat gcgcctctcc
     1081 catttcttca tcgaccggcc gatcttcgcg acggtgctga gccttttcat caccatcctc
     1141 ggcgccatcg cctatgtcgc gctgcccgtc gcgcaatatc ccgagatcgc gccgccgacc
     1201 atccaggtca ccgccagcta tccgggcgcc tccgccgacg tcgtcagcga gaccgtggcg
     1261 acgccgctcg agcagcagat caacggcgtc caggacatgc tctacatgag cagccaggcg
     1321 acgggcgatg gcaagctgac gctcaccgtg accttcgcgc tcggcaccaa tctcgacacc
     1381 gcgcaggtgc tgacgcagaa ccgcgtctcg gtcgccgtgc cgcggcttcc cgacgtggtg
     1441 cagcgcttgg gcgtcacggt gaagaagaac tcgcctgaca tgctgatggt ggtccatctc
     1501 tattcgcccg acgggtcgcg cgaccagctc tacatgtcga actacgcgac cttgcaggtg
     1561 aaggacgtgc tctcgcgcct cgacggcgtc ggcgacgtca atatcttcgg ggcgcgcgac
     1621 tattcgatgc gcatctggct cgaccccgac aaggtggcgt cgcgcaacct gaccgccggc
     1681 gacgtcgtgg cggcgctccg tgcgcagaat gtgcaggtct cgtcgggcgt gctcggccag
     1741 ccgcccctgc cgttgcccgg cgccttccag ctcaatgtgc agacgctggg acggctcatc
     1801 gacccggcgc aattcggcga tatcatcatc aagtccgacg gcgctggccg tgtcacgcgg
     1861 gtgcgcgaca tcggacgcgt cgagctcggt gctcaggact acggcgccaa cggctatctc
     1921 gacgcgcgga acgccgtgcc gctgctgatc tatcagcagc cgggatcgaa cgcgctggcg
     1981 accgccgacc atgtccgctc gacgattcag gaattgtcgc agcatttccc gcagggcctc
     2041 aaatacgaca tcgtctacga tcccacgcgc ttcatcgccg agtccgtgcg cgaagtcgtg
     2101 aagacgatcg ccgctgccgt cgccctcgtg gtcctcgtcg tgatcctctt cctgcagacc
     2161 tggcgcgcct cgatcatccc gatcgtggcc attccgatct cgctcatcgg cactttcgcg
     2221 gtcctcgccg cgctcggatt ctccttgaac agcctctcgc tcttcggcct cgtgctcgcc
     2281 gtcggcatcg tcgtcgacga cgccatcgtc gtcgtcgaga acgtcgagcg caatttgagc
     2341 gccggcatga gcccgcgcga agccgcgcac cgcaccatgg acgaggtcgg cggcgcgctc
     2401 atcgccatcg ccttgacgct ctgcgcggtg ttcgtgccgg cgaccttcat gagcggcatc
     2461 tcggggcagt tcttccgcca attcgccgcg accatcgccg cctcgacggt aatctcgtgc
     2521 ttcatctcgc tgacgctgag cccggcgctt tgcgccttgc tgttgaagcc gcatctcagg
     2581 gctgcggatc agccgcgccg cccgctgctc gcgcggccgc tcttcgcctt ttttcgcggc
     2641 ttcaatcgcg ccttcgccgc ggtcgcggag cgctacggca gcctcaccgc gcggctcgtg
     2701 cgcatggccg cgatcgtgct cgtcgcttat gcggggctga tcggccttac cgcctggcag
     2761 ttcggccgcg cgccgaccgg cttcatcccc gaacaggacc agggatacct gatcaccgtc
     2821 atccagctgc cgccgggcgc ctcactggcg cggacagatg cggttgtccg caaggcagcg
     2881 gagatcatcc tggcgacgcc cggcgtgcgt cacgccgttc ccttcgccgg cttcgatggc
     2941 gccacgttca ccaacgcgcc caatgccggc gccatcttct tttcctacgg cacattggcg
     3001 ctcggcccct tcgaggagcg ccccgcccga ggcgtgagcg gggatgcgat gattgcgcag
     3061 atgcgccagc gccttgccgc gatccaggat gccttcatca tcaccatcaa gccgccgccg
     3121 gtgcgcggca tcggcaccgc cggcggcttc aagatgatgg tcgaggaccg ccaggaccag
     3181 gggtcgagcg cactcgatgc ggcggttcag gacctcgccg gcgccgccaa ccaggtgccg
     3241 ggcctcgtcg gcgtgttctc gctcttcaac acgcgcactc ccgccgtcta tgccgacatc
     3301 gatcgcgagc gggcagagaa gctgggcgtc tcgcccgaca aggtgttcga cgcgctccag
     3361 gtttatctcg gctcggccta cgtcaacgac ttcaactatc tcggccggac ctaccaggtg
     3421 atggcgcaag ccgacgggca gttccgcgcg gacgcccagg acatcgcgcg gctcaagacg
     3481 cgcaacgacg cgggggccat ggtgccgctg ggctcggtgg cgcaattccg cgacgtcacc
     3541 gggccttatc gcgtgccgcg ctacaacctc tatcccgccg ccgagctgca aggcaacacg
     3601 gcgcccggcg tctcctcggg ctatgcgatc gccgcgatgg agcagctcgc cgccgagcac
     3661 ctgccgaacg gcttctcctt cgactggacc gatctcgcct tccagcagaa gcaggcgggc
     3721 gacaccgcgc tcctcatctt cgccgcctcc gtcgtcttcg tctttctcgt gctggccgcg
     3781 caatacgaga gctggacgct gccgctctcg gtcgtgctca tcgtgccgat gtgcctcctg
     3841 gcggcggtca cgggccttat gctgcgcagc atgagcgtcg acatcctggc gcagatcggt
     3901 ttcgtcgttc tcgtcgggct cgccgccaag aacgccatcc tcatcgtcga attcgccagg
     3961 caggacgagg aagcgggcga gagcgtcgcc gaggcggcgg ttcatgccgc gcgcacgcgg
     4021 ctccggccga tcctgatgac gtccttcgcc ttcatcctcg gcgtcgtgcc gctggtgacc
     4081 gccagcggcg cgggcgccga gatgcggcag tcgttgggga cggcggtctt cgccggcatg
     4141 ctcggcgtca ccgccttcgg cctcctcttc acgcccgtct tctacaccgt catgcgccgc
     4201 ctttcccgca cccgctcgcg cggaggcgcg gaggcggctt cccaatcagg gtcgccagcg
     4261 tgacgcggta gcctgtcctt cggaagccga gccccgaaac gattacccgg cgaaacctat
     4321 gcgcgatggt catcacgcca agcagaatct gcgcgtggcg ttggcctgac gcgggcgata
     4381 tggtcgcgct tcgccacgac tcgggccgga gccttggaga gcgagcgcca tgaccttgaa
     4441 actctacgcc atgacctgcg gtcatctcac ctgccagctc ggcccgatgc tggagggcgg
     4501 cgaaggcgag gtggtgctgc cgattccggc ctacctcatc gagcatccga agggaacggc
     4561 gctgttcgac accggcatgc atcccgattg caggagagac cccgccgctc gtgtcggtcc
     4621 ggatgtcgcc cggttcttcc gtttcgacta tcgcccagat gaggatatcg gcgcgcggct
     4681 ccgatcgatc ggccgagatc ctgcgcgcat cgatttcatc gtcaactcgc atttccattt
     4741 cgaccatgtc ggtggcaacg cgctcattcc caatgcgacg atggtggtcc agaaggccga
     4801 atgggaggcg ggaatggatg cggagctcgg cccgcgtctc ggcctcgacc gtcgcgattt
     4861 cgacctcggt catcgcctgc gcaggatcgc gggcgagcac gacctgttcg gtgatggcag
     4921 cgtggtctgt ttcccgactt acgggcacac ccccggccat caatccttgc ggctgcgcct
//
LOCUS       DASYNS010000353         8727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2583489, whole genome shotgun sequence.
ACCESSION   DASYNS010000353 DASYNS010000000
VERSION     DASYNS010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2583489"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..622
                     /locus_tag="VGP50_11015"
     CDS             47..622
                     /locus_tag="VGP50_11015"
                     /inference="COORDINATES: protein motif:HMM:NF033827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV8027953.1"
                     /translation="MNIASVWLLGGDDHEHGHGHHHAHDEEHDDDDHDHDHEHHAEDH
                     AGDGHGAAHRDNNMRAAFVHVIADAAVSVLAIIGLSAGKFLGWVFMDPIMGIMGALVI
                     ANWSYGLVRDTGAILLDMTPDRALTEKLRQAIEGEGDTLTDLHLWRLGPGHLGAILAV
                     TTGQARGPDFYRRRLGGFRALSHVTIEVHSR"
     gene            814..2772
                     /locus_tag="VGP50_11020"
     CDS             814..2772
                     /locus_tag="VGP50_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEV8027954.1"
                     /translation="MAKTQMVDLLGERALVLPALLTAAIAANERAKYILTLLQVAASQ
                     AENPQRPAPSLRAEREACGIDAPEFDRVVARTEYDGGGSYHIPGASRLIALLDDALRA
                     MIAPLAVAAKDGRNRATAHAAFRDRLATLMETRPAIVEDMLTGDLIAALTSARPKAGD
                     GVHAFVMDLHQEINRLQSAVSIMDIAGAKAYGLRDGDRPAVTAFMTGVRRTAPLKFDH
                     PGLDTIAARAGETLLIQNDIGTTEAHVLVVKAEGLDVTVTYSDVHLPRLRFLQALCAE
                     AGIAWEEVRSRPAAGIADGDLFYLAFGRYKAPDAAALNAFLERLGSRIVFLIDWNKAR
                     KRLGLLVPSETAVEILRWAADHDVGHRGFLSLGGERLIYDALEQAVKTPLRYGEPLHE
                     MIGADVARDYLCFVLQITATGLLAGQSEALIRDRVRAELFSHFRSAEQRLLAACSGHA
                     EIIAQLANGLRSALRQGAGDAPDALAQHAARAKLWESRADVVVRDMRSTVRRIAGTEI
                     FCRIVEAADDAADGLEDATFLTRLLFESAPPRELPEPLLALADLLADGAQAFCRTMET
                     AQYVHRGSERDAVQRFLEAADHVVGVEHQTDECERAVTGALMRTPLDSRQLFMLSGIA
                     HHLEAAADALMHASLKLRDHVLGDVMFA"
     gene            3328..4104
                     /locus_tag="VGP50_11025"
     CDS             3328..4104
                     /locus_tag="VGP50_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027955.1"
                     /translation="MSIDPSNIYGAASAALPAPSAMSAAPTHTHAWKDGGPGFHDVLD
                     AINPLQHIPVVSAIYRWLTGDVPGNVAQLAGDTLYGGPVGLATGLFGVAFKEETGKDP
                     GAMAIALLGGGSGAPAAAAAPEPPAAPILAADASAGPATPSLAPAQPPIPLVKAPAAI
                     PLPSAASNFLAQSALHQRSLVGARPVTQAIPLKLSGQTIPLAPLHMPPAAAAPAMTAT
                     APTAAADVPPTADVPQRMLDALDKYRALEQQRGGAVDLRQ"
     gene            complement(4111..4806)
                     /locus_tag="VGP50_11030"
     CDS             complement(4111..4806)
                     /locus_tag="VGP50_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV8027956.1"
                     /translation="MGTGPAPPIAENLAAVRARMAQCAAGAGRDSRDITLVAVSKTHD
                     AGAVAAAIAAGQRVFGENRVQEAQTKYPALRASHADLELRLIGPLQTNKVKEAVALFD
                     VIETLDRPRLAEALKQEFARQHRAPLLFVQVNTGEEPQKAGVPPEDADAFIADCRQRV
                     GLEIAGLMCIPPLDDEPAPHFALLREIARRNGIRSLSMGMSADYEIAIRFGATHVRIG
                     TAIFGARESVAER"
     gene            5144..5758
                     /locus_tag="VGP50_11035"
     CDS             5144..5758
                     /locus_tag="VGP50_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HEV8027957.1"
                     /translation="MHIGRVGLTLILAGLLGGCGLFGGSSQSQQQQSQAQLPPVDAPK
                     HPEDPYHPRADGGDNYTLMNVLFGTGSNSKTNSNSGNNSSSNGPSNGPATGVGVNTYL
                     WHASLDTLSFMPLASADPFGGVIITDWYSPADSQSERFKLNVLILGRELRADAVRCTV
                     FRQKRDATGQWADTPVDTKTGVDLENAILTRARQMRLSTAAVTK"
     gene            5802..8363
                     /gene="leuS"
                     /locus_tag="VGP50_11040"
     CDS             5802..8363
                     /gene="leuS"
                     /locus_tag="VGP50_11040"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV8027958.1"
                     /translation="MSARYNFKEAEARWQEVWRERGTFAVETDPSRPKYYVLEMFPYP
                     SGKLHVGHVRNYTMGDMVARYKRAQGFNVLHPMGWDAFGLPAENAAMASHVHPAEWTS
                     ANIATMRAQLVRMGLSYDWRREIATCHPEYYRHEQKMFLDFLKADLAYRRESWVNWDP
                     VENTVLANEQVIDGRGWRSGALVEKRLLAQWFLRITRYADDLLDALKTLDRWPERVRV
                     MQENWIGRSEGARVLFALKGRNDKLEIFTTRPDTLFGASFCAISPNHPLAQELAAKNP
                     ALAGFIAECNRMGTSEAVLETAEKQGFDTGLKALHPLVPGWELPVYAANFVLMEYGTG
                     AIFGCPGHDQRDLDFARKYRLPVKPVVLPPGADPAHFSIASEAYVGDGTIINSDFLDG
                     LPVAAAKRKVTERLATLNTGQGTVAYRLRDWGVSRQRYWGCPIPVIHCGKCGIVPVPE
                     RDLPVALPQDVTFDRPGNPLDHHPTWKHTTCPSCGGPALRETDTFDTFFESSWYFERF
                     CSPRAANAFDRPDVDYWMPVDQYIGGIEHAVLHLLYSRFFTRALKDCGYLDLKEPFAG
                     LFTQGMVCHETYRDEDGNWLFPEEVTRDTAARLIDAKNRPVTVGRNEKMSKSKKNVVG
                     LETIVDAYGADTARLYLLSDSPPERDLEWTDSGIEGAWRYVSRLHRLVTSPPVPLAPK
                     GTSLPAALSPAAQKARRAIHRAIATVSEQIETFRFNSAVARVRELTNLLEEMTDGPGA
                     SAVMREGVETAVRLVNPMMPHLGEELWHALGHDRLLADEPWPKADPVLLIDETVTVAV
                     QVNGKLRATIELARDADQGTAEAAALADPAVQRALDGKTPRKIIVVPNRIINLVV"
BASE COUNT         1484 a         2983 c         2775 g         1485 t
ORIGIN      
        1 catcgatttc aacgaggcga tcccgattgc cgtggtcggc ctcggcgtga acatcgccag
       61 cgtctggctc ctcggcggcg acgatcacga acacgggcat ggccatcatc acgcgcacga
      121 tgaggaacat gacgacgacg accatgatca tgatcacgag catcatgccg aagatcacgc
      181 cggcgacggc cacggggcgg cgcaccgcga taacaacatg cgcgccgcct tcgtccatgt
      241 gatcgccgat gcggcagtgt cggtcctggc gatcatcggc ctcagcgccg gaaaatttct
      301 cggttgggtg ttcatggatc cgatcatggg catcatgggc gcgctcgtca tcgccaactg
      361 gtcctacggc ctcgtcagag acaccggcgc gatcctgctc gacatgacgc ccgatcgcgc
      421 gctgacggag aagctgcggc aggcgatcga gggcgagggc gacacgctga ccgatcttca
      481 tctgtggcgg ctcggccccg gacatctcgg cgccatcctc gcggtgacga cggggcaagc
      541 gcgcggtccg gatttctatc gccggcggct cggcggcttt cgcgcgctgt cgcacgtcac
      601 gatcgaagtg cattcgcgct gacatccggc actgccgtcg taaaactgtc acgaagcgtg
      661 tgagctgcgg tgtcgttgtg ccgtgaaatg ttgcgcgcct tgcggcagat caaagccacg
      721 ccacgccgtt gtcggcatca tcgacatcgc tcaccgacga accatgctcg tcgcggcgag
      781 accgtgaccg gcaaaacagg cgagacaccg caaatggcga aaacacagat ggtcgatctt
      841 ctgggcgagc gtgccctggt gctgccggcg ctgcttaccg ccgccatcgc cgccaacgag
      901 cgcgcgaagt acattctgac gctgctgcaa gtggcggcaa gccaggcgga gaatccgcag
      961 cgcccggcgc cttccttgcg cgccgagcgc gaagcctgcg gcatcgatgc gccggagttc
     1021 gaccgcgtgg tggcgcgcac cgagtatgac ggcggcggca gctatcatat tcccggcgca
     1081 agccgcctca tcgccctgct cgacgatgcc ttgcgcgcga tgatcgcgcc gctggccgtg
     1141 gcggcgaagg atggacggaa ccgcgcgacg gcccacgccg ccttccgcga tcgtctggcg
     1201 acgctgatgg aaacgcggcc ggcgatcgtc gaggacatgc tgaccggcga tctcatcgcg
     1261 gcgctgacct ccgctcggcc gaaagcgggc gacggcgttc atgccttcgt catggatctg
     1321 catcaggaga tcaaccggct gcaaagcgcc gtctcgatca tggatatcgc cggcgccaag
     1381 gcctatggcc ttcgcgacgg cgaccgcccg gccgtgacgg cgttcatgac cggcgtccgc
     1441 cggacggcgc cgctcaaatt cgaccatccc gggctcgaca ccatcgccgc gcgcgctggc
     1501 gagacgctgc tcatccagaa cgacatcggc acgaccgaag cgcatgtgct cgtcgtcaaa
     1561 gccgaaggtc tcgacgtcac cgtgacctac agcgacgtgc acctgccgcg cttgcgtttc
     1621 ctgcaggcgc tctgtgccga agccggcatc gcctgggagg aggtgcgctc gcgcccggcc
     1681 gcgggcatcg ccgatggcga tctcttctat ctggccttcg gacgatataa ggcgcccgat
     1741 gccgcggcgc tcaacgcgtt tctcgagcga ctggggtcgc gcatcgtctt tctcatcgat
     1801 tggaacaagg cgcgcaaacg cctcgggctt ctcgtgccga gcgagacagc ggtcgaaatc
     1861 ctccgctggg cggcggacca cgatgtcggt caccgcggat ttctctcgct cggcggcgag
     1921 cggctcatct acgacgcgct ggagcaggcc gtgaagacgc cgttgcgcta tggcgagccg
     1981 cttcacgaga tgatcggcgc cgatgtggca cgcgactatc tctgcttcgt gctgcagatc
     2041 acggcgaccg gtctgctggc gggacaatcc gaagcgctca ttcgcgatcg cgttcgcgcc
     2101 gagctgttca gccattttcg ctccgccgag cagcgcctgc tcgccgcgtg ctcgggtcat
     2161 gccgagatca tcgcgcagct cgcgaacgga ctgcgcagcg ctttgcggca aggcgcgggg
     2221 gatgcacccg atgcactcgc gcagcacgcg gcgcgggcca agctctggga gagccgcgcc
     2281 gatgtggtgg tgcgcgacat gagatcgacc gttcgccgca tcgccgggac cgagatcttc
     2341 tgccgcatcg tcgaggcggc cgacgatgcc gccgacggtc tcgaggacgc gacttttctc
     2401 acgcgcctgt tgttcgagag cgcgccgccg cgcgaattgc ccgagccgtt gctggcactc
     2461 gccgacctcc ttgccgacgg cgcgcaggcc ttctgccgga cgatggagac ggcgcaatat
     2521 gtgcaccgcg gcagtgagcg cgacgccgtt cagcgttttc tcgaagccgc cgaccacgtc
     2581 gtcggcgtcg agcaccagac cgacgaatgc gagcgcgccg tgacgggcgc tctcatgaga
     2641 acgccgctcg attcccggca gctcttcatg ctgagcggca tcgcccatca tctcgaggcg
     2701 gcggccgacg ccctgatgca tgcgagcctg aagcttcgcg atcacgtgtt gggcgacgtc
     2761 atgttcgcct gagcggcaag cgcctcgaat ttattttgcc gacgagtttc gacaaattat
     2821 cgcgatgccg ctcgacagcc gcgcgaagcc gcgtgtgcgg cggcgggcgc cggcgtggta
     2881 acggagctgt cacgatttgc gcctagcgtt ttgccaggag ggtcttcggc atgcgttgga
     2941 agaacagcga cgaagaagtg acggggctcc ttgccatctt catgatcctc gcgacgctcg
     3001 cggtcatcgg ctcctatctc tggcgtttgg cccactagag cacgtcccat ttacgtggaa
     3061 ccgctccttc gtcgtcgtcc ccgcgaaagc ggggacccag gggcgagcga cgtgccggtg
     3121 gccctggatt cccgctttcg cgggaatgac ggtgttatca ggtatcgatt ccacctgctc
     3181 gagatctaat ctatcgagcg acggcgccag acgcgttttg ccgcgcctgc cggcagttct
     3241 tgccgagcgc ggcgtctcct ctttccgaat ctccccgaag atccgccagt ttcgattggc
     3301 atcaagcttg cgtagcagct tgcgccaatg tcgatcgatc cttccaacat ctatggggcg
     3361 gccagcgcag ccctccccgc cccgtccgcc atgtccgcgg cgccgacgca cacgcacgcc
     3421 tggaaagacg gcgggcccgg gttccacgac gtcctcgacg cgatcaatcc gctgcagcac
     3481 atccccgtgg tgagcgcgat ctatcgttgg ctgaccggcg acgtgccggg caatgtcgcg
     3541 cagctcgccg gcgacacgct ctacggcgga cccgtcggcc tcgccaccgg gctcttcggc
     3601 gtcgccttca aggaggagac cggaaaggat cccggcgcaa tggccattgc tctgctcggc
     3661 ggcggcagcg gcgcgccagc ggcggcagcc gcgcccgagc cgcccgctgc accgatcctg
     3721 gcggccgacg cttcggccgg gcccgccacg ccgtcgctcg cgccggcgca gccaccgatc
     3781 ccgctcgtca aggcgccggc cgcaataccg cttccctccg cggcaagcaa ttttctcgcg
     3841 cagagcgcct tgcaccagcg cagcctcgtg ggtgcgcgtc ccgtcaccca ggcgatcccg
     3901 ctcaagctca gcggccagac catcccgctc gcgcccttgc atatgccgcc cgccgccgcc
     3961 gcgcccgcga tgacggccac agcgccaacg gccgcggccg atgtcccgcc caccgccgac
     4021 gttccgcaac gcatgctcga tgcgctcgac aagtatcgcg cgctcgagca gcaacggggc
     4081 ggcgccgtcg atttgcgcca atagtatcgc tcatcgctct gcgacggact cgcgcgcgcc
     4141 gaagatggcc gtgccgattc tcacatgcgt ggcgccgaag cggatggcga tctcgtaatc
     4201 ggcgctcatg cccatgctga gcgagcgaat gccgttgcgc cgcgcgatct cgcgcagcaa
     4261 tgcgaaatgc ggcgccggct cgtcgtcgag cggcgggatg cacatcaggc cggcgatctc
     4321 gagaccgacg cgctgccggc aatcggcgat gaaggcatcg gcatcttccg gcggcacgcc
     4381 cgccttctgc ggctcctcgc cggtattgac ctggacgaag agcagcggcg cacggtgctg
     4441 gcgcgcgaat tcctgcttca gcgcttcggc gaggcgcggc cggtcgagag tctcgatgac
     4501 gtcgaagagc gcgaccgctt ccttcacctt gttggtctgc aaaggaccga tgaggcggag
     4561 ctcgagatcg gcatggctgg cgcgcagcgc cggatatttc gtttgcgcct cttgtacgcg
     4621 attctcgccg aagacgcgct ggcccgcggc gatcgccgca gcgaccgcgc cggcatcatg
     4681 ggtcttgctg acggcaacga gcgtgatgtc gcggctgtcg cggccggcgc ctgccgcgca
     4741 ttgcgccatc cgcgcccgca cggccgcgag gttctccgcg atcggcggtg ctggcccggt
     4801 ccccatggcc gcgcgaccat ggcccgagcc ggcgggcagc gtcaagggac gaagggacgc
     4861 gcgccgcctc ttccccaagg ccagggctat cgcatatggg tttcaccacg gaggcacgga
     4921 gaagaggggt tcggcgcgcg aagcgcgcca ttcttcgctt ctccgtgctc tccgtgcctc
     4981 cgtggtcgat ctttgctatc gccgtcccgg caggctatgc gattgccgta ttcccaagct
     5041 ggagccaaga tttagcagca tttcctgacc ctgtcggtgt agtatcggcc ggcctttcgg
     5101 ggggcggggg ctccttcgac ggaaaagtgc aggagagtat gaaatgcata tcggccgtgt
     5161 cggtttgacg ttgattcttg ccggcttgct cggggggtgc ggcttgtttg gcgggtcttc
     5221 ccagagccag cagcagcagt cgcaggccca gcttcctccg gtcgacgcgc cgaagcaccc
     5281 cgaggatccc taccacccgc gtgccgacgg cggcgataac tatacgctga tgaacgtcct
     5341 cttcggcacc ggcagcaaca gcaagaccaa cagcaacagc ggcaacaaca gcagcagcaa
     5401 cgggccgagc aatggccccg ccaccggcgt cggcgtcaac acctatctct ggcacgccag
     5461 cctcgacacg ctctccttca tgccgctggc atcggccgat cccttcggcg gcgtcatcat
     5521 caccgactgg tacagcccgg ccgacagcca gagcgagcgc ttcaagctca acgtcctgat
     5581 cctcgggcgc gagctgcgcg ccgatgccgt gcgctgcacg gtcttccgcc agaagcgcga
     5641 tgcgaccggc cagtgggccg ataccccggt cgacaccaag accggcgtcg acctcgagaa
     5701 tgcgatcctg acccgcgcgc ggcagatgcg gctcagcacg gcggcggtga cgaagtagag
     5761 ccgcgcgcgg cgttattgcg ggagttcgag agcggagcct gatgtcggcg cgctacaatt
     5821 tcaaggaagc cgaggcgcgg tggcaggagg tgtggcgcga gcgcggcacc ttcgctgtcg
     5881 agaccgaccc gagccggccg aaatactacg tgctggagat gtttccctac ccctccggca
     5941 agcttcatgt cggccacgtc cgcaactaca ccatgggcga catggtggcg cgctacaagc
     6001 gcgcgcaggg cttcaacgta ctccatccga tgggctggga cgccttcggt ctccccgccg
     6061 agaacgccgc catggcgagc catgtccatc cagccgaatg gaccagcgcc aacatcgcca
     6121 ccatgcgcgc ccagctcgtc cgcatggggc tctcttacga ctggcggcgc gagatcgcca
     6181 cctgccatcc ggagtattac cggcatgagc agaagatgtt cctcgacttc ctcaaggccg
     6241 acctcgctta tcgccgtgag tcctgggtca actgggatcc ggtcgagaat accgttctcg
     6301 ccaacgagca ggtcatcgac gggcgcggct ggcgctctgg cgccctggtc gagaagcggc
     6361 tgctcgcgca gtggttcctg cgcatcacgc gctacgccga cgatctcctc gatgcgctga
     6421 agacgctcga ccgctggccc gagcgggtcc gcgtcatgca ggagaattgg atcggccgct
     6481 cggaaggagc gcgcgtgctc ttcgccctca aagggcgcaa cgacaagctc gagatcttca
     6541 ccacccgtcc cgacacgctc ttcggcgcct ccttttgtgc gatctcgccc aatcaccccc
     6601 tggcccagga gctcgcggcg aagaacccgg cgctggctgg gttcatcgcc gaatgcaacc
     6661 gcatgggaac gagcgaagcc gtgctggaga cggccgagaa gcagggcttc gacaccgggc
     6721 tgaaggcgct tcacccgctg gttccaggct gggaactgcc ggtctacgcc gccaatttcg
     6781 tgctcatgga gtatggcacg ggcgccatct tcggctgccc cggccatgac cagcgcgatc
     6841 tggatttcgc ccgcaaatac cgcctgccgg tcaagccggt ggtgctgcct cccggcgcag
     6901 acccggcgca tttttccatc gccagcgagg cttatgtcgg agacggaacg atcatcaact
     6961 cggattttct cgatgggctc cccgtcgccg cggcgaagcg caaggtgacg gagcggcttg
     7021 cgacgctcaa caccggccaa ggcaccgtcg cctatcgcct gcgcgactgg ggcgtctcgc
     7081 gtcagcgcta ctggggctgc ccgatccccg tcattcactg cggtaaatgc ggtatcgttc
     7141 cggtaccgga acgagatctg ccggtagctt tgccgcagga cgtgaccttc gatcggccgg
     7201 gtaacccgct tgaccaccac ccgacctgga agcacacgac ctgcccaagc tgcggcggcc
     7261 ccgccctgcg cgagaccgac accttcgaca cgtttttcga atcctcctgg tatttcgagc
     7321 gcttctgctc gccgcgggcg gccaatgcct tcgatcgccc cgatgtcgat tactggatgc
     7381 cggtcgacca gtatatcggc ggcatcgagc acgccgttct ccacctgctt tactcgcgct
     7441 tcttcacacg ggctctcaaa gattgcggct atctcgacct gaaggagccg ttcgccggcc
     7501 tcttcacgca agggatggtg tgccacgaga cctatcgcga cgaggacggc aactggctct
     7561 tccccgaaga ggtgacgcgc gacaccgccg cgcgcctgat cgacgcaaag aaccgtccgg
     7621 taaccgtcgg ccgcaacgaa aagatgagca aatccaagaa gaacgtcgtc ggcctcgaaa
     7681 ccatcgtcga tgcctatggg gcggacaccg cccgcctcta tctgctgtcg gacagcccgc
     7741 ccgagcgcga cctcgaatgg accgattccg gcatcgaagg ggcgtggcgc tatgtcagcc
     7801 ggctccaccg cctggtcacg tcaccgccgg tgccgctcgc gcccaaaggc acgagcctgc
     7861 cggccgcctt gtcgcccgcc gcgcagaagg cccgccgcgc aatccatcgt gcgattgcca
     7921 cggtcagcga gcagatcgag acctttcgct tcaacagcgc cgtcgcgcgc gtgcgcgagc
     7981 tcaccaacct gctcgaggag atgaccgatg ggccgggcgc atccgcggtg atgcgcgaag
     8041 gcgtcgagac ggcggtgcgc ctcgtcaacc cgatgatgcc ccatctcggc gaagagctct
     8101 ggcacgcgct cggccatgac cggctgctcg ccgacgagcc ttggccgaaa gccgatcccg
     8161 tgctactcat cgacgagacc gtgacagtag cggttcaagt gaatggcaag ctgcgcgcca
     8221 cgatcgagct tgcacgcgac gcggaccaag gcaccgccga agccgccgcg ctggcggatc
     8281 ctgccgtgca gcgcgcgctc gacggcaaga cgccgcgcaa gatcatcgtc gtccccaacc
     8341 gcatcatcaa cctcgtcgtt tgagggaagc tttgaccacg aaggcacaga gagaagagga
     8401 acgcagaggc cgcggaggtt gcgcggaggg cgcggaggat gcgcttcctc cgcgtcctct
     8461 gcggcgcctc cgcgttcctc ttctaagcag agttgcacag atcgcgacac atatgcacaa
     8521 atcccctctc cccggagggg agagggggga atgtgtgcgc ggtcaaagca atgctgctta
     8581 gttattcttc ctctctgtct ctctgtcgct tgaggaaaaa agattgacca cagagacaat
     8641 gagacatgga gaaaagaaga agggggtgct tcgcgtgccg agaattcttc ctcactgtct
     8701 caccgtctct gtggtcaatc ttcttat
//
LOCUS       DASYNS010000354         6213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3988282, whole genome shotgun sequence.
ACCESSION   DASYNS010000354 DASYNS010000000
VERSION     DASYNS010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3988282"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(500..742)
                     /locus_tag="VGP50_11045"
     CDS             complement(500..742)
                     /locus_tag="VGP50_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027959.1"
                     /translation="MAESEGLAIAGYMLTYTLIRKLEAKNVLTRLEAEELIEGAIRQL
                     QAGGPREPALQQAHSILKETLDIVHEGAEPPSEGGH"
     gene            complement(890..2467)
                     /locus_tag="VGP50_11050"
     CDS             complement(890..2467)
                     /locus_tag="VGP50_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEV8027960.1"
                     /translation="MPRLLKAGAALMLLGACAVGPDFAKPAPPPVAGYTREPLAAETS
                     ATADISGGAGQRLVQGLDLPGQWWTLFHSEALDRLIERALAANPDLQAAQAALRVANE
                     NVYAQEGAYFPTVAGSFTPSRQKTATGSLSPASASGNPYFSLYTAQLSVSYVPDVFGL
                     NRRTVESLAAQAENQRFQVEATYLTLTSNVVTAAVQEAALRGQIAATQEIIRFETELL
                     QLLQRQLALGQIAEVDVAAQDAALAQAEAALPPLLKQLAQQRDLLTALTGAFPSEEPA
                     ETFDLSGLQLPEDLPVSLPARLVEQRPDVRAAEANLHAQSALIGVAVANRLPNLTLTA
                     SAGSAANTISRMFTTGTGFWSVAGSLTQPIFEGGTLLHRERAARAAYEQSAALYRSTV
                     VAALQNVADALRALEFDADALKAAARSERAAQRSLELVRAQLRLGAVNYLALLNAQTT
                     YQQALINLVQAQAARFADTAALFQAMGGGWWNRGDVAATADPEVTTAAAQPAAAVPGD
                     APPTPARRDGLANASGL"
     gene            complement(2547..5729)
                     /locus_tag="VGP50_11055"
     CDS             complement(2547..5729)
                     /locus_tag="VGP50_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEV8027961.1"
                     /translation="MNAVVGFALKQRILMVVLLFFLLGAGVISFLKLNIEAYPDPVPP
                     LVDIITQSSGQSAEEIERYITIPIEVQMAGIPHITAIRTISLFGLSDVKVQFTYDFTY
                     EQAEQWVINRLSQLPPLPNGAQPQISPTSPIGEIYRYRVAGPPGYSVTDLKTIQDWIL
                     ERRFKAVPGVIDVNGWGGKTKTYAVTVDLNKLIAYGLTLPQMLQVLNNSNINVGGQTV
                     NFGAQSAVVRGVGLIHSMDDIRDTMLTANNGSPVLVKDVAAVTVDHEPRLGIAGQDND
                     DDIVQGIVLMRRGAESLPTLKLVEAEIERINAGNILPPGVHIEKIYNRTDLINVTTHT
                     VLHNMIVGILLIFAVQWIFLGDLRSAIIVATTIPFALFFAIIILLVRGESANLLSVGA
                     IDFGLIVDATVIMVENIFRHLAQRPEERFDRSPLLHRVRSAVEFRGKFAVIANSAVEV
                     NQAIFFSAAIIIAGFVPLFTLSGIEGHIFGPMAKTYAYAIAGGLIATFTVSPALAALL
                     LPDRVEETETFIVRWLHRAYRPALEFALANRVLTLGAAALLFVLAALGVRTLGLEFLP
                     KLEEGNLWIRASMPPSISLEAGNSYVNEMRRVIKGFPEVVTVISQNGRPDDGTDATGF
                     FNAEFYVPLKPFDTWPAGVDKDKLVDEVNEALEARFPGVEFNFSQTIEDNVEEAASGV
                     KGENSVKLFGNDLEGLERTAAKIKDVMATVPGVTDLAIFNSLGQPTVRIDVDRARAAR
                     FGLAPGDVNATVQTAVGGQAAGNLYEDGSDRNFPIMVRLAPEYRQSLEAIRQITIGAQ
                     NPSGNGVVQIPLSDVAQVSLVSGASFIYREQQERYIPIKFSVRDRDLGSAVLEAQQKV
                     AQQVPLAGYRLDWAGEFGNLQEAVQRLAIVVPISLLLICILLYVNFASVSDMLLAASV
                     MPMSLVGGIFALYVTGTPFSVSAAIGFVALFGIAAMDGIIVLSYFNRMIADGLDRASA
                     LLRTAEVQMRPVVMTCVAACVGLLPAAISTGIGSQVQKPLALVVVGGILVAPVLILIV
                     LPVLIDLFSRRGQEAEGGDHTSSMQTKPAE"
     gene            complement(5748..>6213)
                     /locus_tag="VGP50_11060"
     CDS             complement(5748..>6213)
                     /locus_tag="VGP50_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV8027962.1"
                     /translation="NLSTVWLVANVREADAPLMRVGEAVEVHVLAFPGRVFSAKIAYV
                     APSVDPNTHRLMVRAEVDNADGALKPEMFARFSIITDKDSAAPSVPADAVVYEGEAAR
                     VWIARDDKSLALRDIRVGRIEDGAVEVLAGLEAGETVVTSGSLFIDRAAKGD"
BASE COUNT         1060 a         2018 c         2108 g         1027 t
ORIGIN      
        1 ggcgagcgcg gccgcgccga cggccaggct aagcaccaaa ccacgcatcg aagattctcc
       61 ccctcgttaa tgtccgcggc gttcagaccg ctgtgggctc gatccatcgc tggcaacgcc
      121 ggctgtaccc cgcgttgcgc cccccgatgc ggcgcccgcc aatcgatgta agtcccctgt
      181 aacatgatga tcaataaaga ggcgagatcg atgtttgcag tgcagcatta aaacggtata
      241 tagattttaa ccaggatata tttgctgcct acagatcatg acttgaggtt gattttcagt
      301 agttttctgc tatggataat ttattgtctc cagaacaaag atatatgagt ctttaatgct
      361 ccccgtagcg gcgtacggcc tcgcctgccg ccccgttatc agggagcaga gaaagtgcat
      421 cctgcgacgg tatgtgcctt cacatcgggc gcggtctcga cgggccggga ggggacgcgc
      481 gtaagtcccg cgcggtcgtt cagtgcccgc cctcggatgg cggctcggcg ccttcatgca
      541 cgatgtcgag cgtctccttc aggatcgagt gcgcctgctg cagcgccggc tcgcgcgggc
      601 cgccggcctg aagctggcgg atcgctccct cgatcagctc ttccgcctcg agccgcgtca
      661 ggacgttctt ggcctcgagc ttgcggatca gcgtataggt cagcatgtag ccggcgatcg
      721 ccagcccctc ggattccgcc atgatgattt gcctctctca ggccgccgtc aggcagcaaa
      781 gccgcgcaaa ataacgcgcc cctgcataaa gaaacgagac ggatcggccc gcatcgccgc
      841 tgcggcggag cgccgccaca acgccgctcc ccttgatcga gatctgccgc taaagcccgg
      901 atgcgttggc gagcccgtcg cgccgagcgg gcgtcggagg agcgtcgccc ggcacggccg
      961 cggcgggctg cgccgcggcg gtggtcacct cggggtcggc cgtcgccgcg acatcgccgc
     1021 ggttccacca gccaccgccc atcgcctgga acagcgccgc cgtatcggca aagcgcgcgg
     1081 cctgcgcctg cacgagattg atcagtgcct gctgatacgt ggtttgcgca ttgagaaggg
     1141 ccagatagtt gacggcgccg aggcggagct gcgcgcgcac cagctcgagg cttcgttgcg
     1201 cggcccgctc cgaccgcgcc gccgctttca gtgcgtcggc atcgaattcg agcgctctca
     1261 gcgcatcagc gacattctgc agcgccgcca cgacggtgct gcgatacagc gccgccgact
     1321 gctcgtaggc ggcgcgcgcg gcgcgctcgc ggtgcagcag cgtgccgcct tcgaagatcg
     1381 gctgcgtcaa gctgccggca acactccaga atccggtgcc ggtcgtgaac atgcggctga
     1441 tcgtgttcgc ggcgctgccc gcgctcgccg tcaaagtgag attcggcagc cggttggcga
     1501 cggcgacgcc gatgagtgcg ctctgggcgt gcagattggc ctcggccgcg cgcacatccg
     1561 ggcgctgttc cacgagacgc gccggcaggc tgaccggcag atcttccggc agctgcaagc
     1621 ccgagagatc gaaggtctcc gccggctcct cgctgggaaa ggcgccagtg agcgccgtca
     1681 gcaggtcgcg ctgttgcgcc agctgtttca agagcggcgg cagcgccgcc tcggcttgcg
     1741 ccagcgccgc gtcctgggcc gcgacgtcga cctcggcgat ctggcccaac gccaactgcc
     1801 gctgcaacag ctgcaacagc tcggtctcga agcggatgat ctcctgcgtc gccgcgatct
     1861 gcccgcgcag cgccgcctcc tgcactgccg ccgtcaccac attcgaggtc aaggtgagat
     1921 aggtcgcctc gacctggaag cgctggttct cggcctgcgc cgcgagcgac tccacggtcc
     1981 gccggttgag gccgaacacg tcgggcacat aggagacgct gagctgcgcg gtatagaggc
     2041 tgaaataagg attgccggag gccgaggcgg gagaaaggct gccggtcgcc gtcttctgcc
     2101 ggctcggcgt gaaactgccg gccaccgtcg ggaaataggc gccctcctgg gcgtagacat
     2161 tctcgttggc gacgcgaagc gccgcctgcg ccgcctgcag gtccgggttg gccgcgagcg
     2221 cgcgctcgat gagccggtcg agcgcctccg agtggaacag cgtccaccac tgtcccggaa
     2281 ggtcgaggcc ttggacgagg cgttgccccg ccccgccaga aatgtcggcg gtcgccgatg
     2341 tctcggcagc gagcggctct cgcgtgtaac cggcgaccgg cggcggtgcc ggcttggcga
     2401 aatcgggccc gaccgcgcaa gccccgagca gcatcagcgc ggcgccagcc ttcagcaatc
     2461 gtggcatcac ccccctcctc cccccttgaa aggaaggatt cgggaagggg gtggacgccg
     2521 aatgtgcgat tccgaccacg cgacgcctac tccgccggct tcgtctgcat cgacgacgta
     2581 tggtccccgc cctcggcctc ttggccgcgg cgcgagaaga gatcgatcag caccggcagc
     2641 acgatgagga tgagcaccgg cgccaccagg atcccgccga ccacgacgag cgccagcggc
     2701 ttctgcacct gcgagccgat gccggtggag atggccgcgg gcagcagccc gacgcaggcc
     2761 gcgacgcagg tcatgaccac cggccgcatc tgcacctcgg ccgtgcgcag aagggcgctc
     2821 gcccggtcga gcccgtcggc gatcatgcgg ttgaagtagg aaagcacgat gatgccgtcc
     2881 atcgccgcga tgccgaaaag ggcgacgaag ccgatcgccg ccgagacgct gaaaggggtg
     2941 cccgtgacat agagtgcgaa gatgccgccg accagcgaca tcggcatgac gctggcggcg
     3001 agcagcatgt cgctgaccga agcgaaattc acatagagca ggatgcagat gagcagcagg
     3061 ctgatcggca cgacgatggc gaggcgctgc accgcctcct gcagattgcc gaactcgccg
     3121 gcccagtcca ggcgatagcc ggcgagcggc acctgctgcg ccaccttctg ctgcgcctcg
     3181 aggacggcgc tgccgaggtc gcggtcgcgc acgctgaact tgatcgggat atagcgctcc
     3241 tgctgctcgc ggtagatgaa cgaggcgccg gacaccagcg agacctgggc gacgtcgctc
     3301 aaggggatct ggacgacgcc attgccgctc gggttttgcg cgccgatcgt gatctggcga
     3361 atcgcctcga ggctctgccg gtattcgggc gcgaggcgga ccatgatcgg gaaattgcgg
     3421 tcgctgccgt cctcatagag gttgcccgcg gcctggccac cgaccgcggt ttggaccgtg
     3481 gcgttgacgt cgcccggcgc caggccgaag cgcgcggcgc gcgcacggtc gacgtcgatg
     3541 cgcacggtcg gctggcccag tgaattgaag atcgcgaggt cggtgacacc gggcaccgtc
     3601 gccatcacat ccttgatctt ggcggcggtc cgctcaaggc cctcgaggtc gttgccgaag
     3661 agcttcaccg agttctcgcc tttgacgccc gaggcggcct cctcgacgtt gtcctcgatg
     3721 gtctgcgaga aattgaactc gacgcccggg aagcgcgctt ccagcgcctc gttcacctcg
     3781 tcgacgagct tgtccttgtc gacgccggcg ggccaggtgt cgaagggttt gagcggcacg
     3841 tagaactcgg cattgaagaa ccccgtggcg tcggtgccgt cgtcggggcg gccattctgc
     3901 gagatgacgg tgacgacctc ggggaatccc ttgatcacgc gccgcatctc gttgacgtag
     3961 ctgttgcccg cttcgagcga gatcgagggc ggcatgctgg cgcggatcca gaggttgccc
     4021 tcctcgagct tgggcaggaa ctcgaggccg agggtgcgca cgccgagcgc ggcaaggacg
     4081 aacagcagcg ccgcggcgcc cagcgtcagc acgcggttcg cgagcgcgaa ttcaagggcg
     4141 gggcggtagg cgcggtgcag ccagcgcacg atgaaggtct cggtctcctc gacgcggtcc
     4201 ggcagcagca gcgccgcgag cgccggcgac accgtgaagg tggcgatgag gccgccggcg
     4261 atggcatagg cataggtctt cgccatcggc ccgaagatgt gcccttcgat tcccgacagc
     4321 gtgaagagcg gcacgaagcc ggcgatgatg atggccgccg agaagaagat cgcctgattc
     4381 acctcgacgg ccgaattggc gatgacggcg aatttgccgc gaaactcgac ggcgctccgg
     4441 acccggtgca gaagcggcga ccggtcgaag cgctcctccg gccgctgcgc cagatgccgg
     4501 aagatgttct cgaccatgat cacagtcgcg tcgacgatga ggccgaaatc gatggcgccg
     4561 accgacagca ggttcgccga ttcaccgcgc accagcagga tgatgatggc gaagaacagc
     4621 gcgaagggaa tggtggtggc gacgatgatg gcgctgcgca aatcgccgag gaagatccac
     4681 tgcaccgcga agatcagcag gatgccgacg atcatgttgt gcagcacggt gtgcgtcgtg
     4741 acgttgatga ggtcggtgcg attgtaaatc ttctcgatat ggacgccggg cggcaggatg
     4801 ttgcccgcgt tgatccgctc gatctcggcc tcgaccagtt tcagcgtcgg caggctctcc
     4861 gcgccgcggc gcatcagcac gatgccttgg acgatgtcgt cgtcgttgtc ctggccggcg
     4921 atgccgaggc gcggctcatg gtccaccgtc accgcggcga cgtccttgac cagcaccggc
     4981 gagccgttgt tggccgtcag catggtgtcg cggatgtcgt ccatggagtg gatgagcccg
     5041 acgccgcgga cgaccgccga ttgcgcgccg aaattcaccg tctggccgcc gacattgatg
     5101 ttgctgttgt tgaggacctg cagcatttgc ggcagcgtca ggccgtaggc gatgagcttg
     5161 ttgaggtcga cggtcaccgc ataggtcttg gtcttgccgc cccagccatt gacgtcgatg
     5221 accccgggca cggccttgaa gcgacgctcc aggatccagt cctggatggt cttgaggtcg
     5281 gtgacggaat agccgggcgg tcccgccacg cggtagcggt agatctcgcc gatcgggctc
     5341 gtcggcgaga tctgcggctg cgcgccgttc ggcagcggcg gaagctgcga caggcggttg
     5401 atgacccatt gctcggcctg ctcataggtg aagtcgtagg tgaactgcac cttgacgtcg
     5461 ctgaggccga agagcgagat cgtgcggatc gcggtgatgt gcggtatgcc cgccatctgc
     5521 acctcgatcg gaatggtgat gtagcgctcg atctcctcgg ccgactggcc cgagctctgc
     5581 gtgatgatgt cgacgagcgg cggcaccggg tcgggatagg cctcgatgtt gagcttgagg
     5641 aagctgatga cgcctgcgcc caacaggaag aagagcagca cgaccatcag gatccgctgc
     5701 ttcagggcaa agccgacgac ggcattcatg tcgaaggttc cggacgctca gtcgcctttg
     5761 gcggcgcggt cgatgaagag gctgccgctg gtcaccacgg tctccccggc ctccagcccc
     5821 gcgagcacct cgaccgcgcc gtcttcgatc cgcccgacgc ggatgtcgcg gagcgccagc
     5881 gacttgtcgt cgcgcgcgat ccagacccgg gccgcttcgc cctcgtagac cacggcatcg
     5941 gcgggcacgg aaggcgccgc cgagtcctta tcggtgatga tgctgaagcg cgcgaacatc
     6001 tcgggcttga gcgcgccgtc ggcattgtcg acctccgccc gaaccatcag gcgatgggtg
     6061 ttgggatcga ccgagggcgc gacatacgcg atcttggcgc tgaagacgcg gcccggaaag
     6121 gccagcacat gcacctcgac cgcctcgccg acgcgcatca gcggcgcgtc cgcctcgcgc
     6181 acattggcga cgagccagac ggtcgacagg ttg
//
LOCUS       DASYNS010000355        15192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_244234, whole genome shotgun sequence.
ACCESSION   DASYNS010000355 DASYNS010000000
VERSION     DASYNS010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 15192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15192
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244234"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VGP50_11065"
     CDS             complement(<1..769)
                     /locus_tag="VGP50_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8027963.1"
                     /translation="MRDNGRIAEIARPPLRLSIDPRLLAAAESVAIPLAALAAALLVF
                     GVFIALCRVNPLSAYGLMYKGAFGSWFSWQNSLIRAAPLVLTALCTALPAQLGLVVIG
                     GEGALVVGGLAAVAAALSLQGMAPLGVQLAMAAAGMAAGGAWIALVGALRHYRGVNET
                     ISSLLLVYIAIALLNHLVEGPMRDPASLNKPSSPGIGDANMIGMIPGTEVHWGLVFGL
                     VAAVLAYVFLYRTTFGLAARVVGGNVRAAKMVGLPVGR"
     gene            complement(766..1884)
                     /locus_tag="VGP50_11070"
     CDS             complement(766..1884)
                     /locus_tag="VGP50_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV8027964.1"
                     /translation="MEYRISRRDLLLGASALGAAGTLAGLLPARRALAANLVVGFIYV
                     GPRDDYGYNQAHAQGAAAVKKMAGVSVLEEENVPETVAVEKTMESMINLDGATLLFPT
                     SFGYYNPYMVKIAAKTPNVRFEHCGGLWSEKDPTNAGSYFGYIDECQHLSGIVAGYTS
                     KTGKLGFVAAKPIPQVLRNINAFTLGARMVNPDITTQVIFTGDWALPIKEAEATNSLA
                     NQGIDVVTCHVDSPKVVVETAERRGIMVGGYHANQSTLAPKGYLTGAEWNWEELYPRF
                     IKMFMAGETIPNMYRGGLKEGLVKTSAYGPLVGDKARQHADAVKAKFMAGDYVIFKGP
                     IKDNKGNAVIAEGTAHGQTDLWLEQMNWLVDGVIGSAA"
     gene            2111..3943
                     /gene="atzF"
                     /locus_tag="VGP50_11075"
     CDS             2111..3943
                     /gene="atzF"
                     /locus_tag="VGP50_11075"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HEV8027965.1"
                     /translation="MARNRPSGAPSLDLIRLREHFERGGLTVAALIEEIIARLTAAGD
                     DKVWISRASDDALRVRAAALDARRAVDPQLLARLPLFGVPFGVKDNIDAAGFATTAAC
                     PAFSYLPAASAPSVARLLEAGAVLVGKTNLDQFATGLVGTRSPYGVPRNPFDDRYIPG
                     GSSSGSAVAVAKGLVSFALGTDTAGSGRVPAAFNNIVGLKPSRGVISARGVVPACRTL
                     DCVSIFALTADDAQRILAICAGYDEADPYSRPMPVPAPAFGRPFRFAVPRAQDREFFG
                     DAENALLFEQALSALEALGGTPVPADFSPFRAAGALLYGGPWVAERLEASEALLRRDP
                     DAVLPVTREIIAGGGTYGAVDVFRAQYRLAELRRESEAVWREADLLVVPTAATIYTLA
                     EIAADPIGRNANLGRYTTFTNLLDLAAVAVPAGFRRDGLPFGLTFLAPAFSDLALCAI
                     AQAYQHSVALPLGATPLRLPPPSAASARPETDGLIRVAVAGAHLTGMPLNGKLLALGA
                     TCVAAAETAPVYRMFLLPQDPPRPGLVRVGEGGRGFAIEIWAMSVAAFGRFVDDVAEP
                     LAIGTVLLADGSKVKGFLCEASAAAEAEDISRFGSWRSFIATRE"
     gene            complement(4011..4715)
                     /locus_tag="VGP50_11080"
     CDS             complement(4011..4715)
                     /locus_tag="VGP50_11080"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV8027966.1"
                     /translation="MINEAPSKAAAGGKAPRRRADLVRDTLADDIIAGRLRAGTRLDE
                     TSLAKRFAVSRTPVREALRELAASGLAESRAHVGAVVTPMTEARFHDIFEAVAELEAV
                     CARLSALKMSAPERYRLEELHRQCGRLVQSGDTELYHAANADFHAAIRQGGQNSVLQE
                     IVGTLRGRFAPLSRSQFRSAGRLAASYAEHDQVVRAILRGDGEQAFQTMRRHHAGVRR
                     SFAEYAASLRPADAAE"
     gene            complement(4712..5458)
                     /locus_tag="VGP50_11085"
     CDS             complement(4712..5458)
                     /locus_tag="VGP50_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HEV8027967.1"
                     /translation="MTYCVGLDLKEGLVLLSDTRTNAGVDNISTFSKMHVTEVPGERV
                     IVLLAAGNLAIAQSVVNLLHEGIDRDGAPETLLTVKSMFRAAQLVGEAVRRIYHIDGA
                     AMQAQNVSFDISFLLGGQIKGRSLRLFQVYAAGNFIEATPDTPFLQVGEHKYGKPILD
                     RAARYDTDLYDGVKLALVSMDSTLRSNLTVGLPIDLLVYRRDALRVELRRRITEDDPY
                     FAMIRERWSQALREAYRAIPRPDWAFAAKP"
     gene            complement(5483..6298)
                     /locus_tag="VGP50_11090"
     CDS             complement(5483..6298)
                     /locus_tag="VGP50_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEV8027968.1"
                     /translation="MRLQVRHQTAYRYETPVAAATQTLRLIPRSYEGLTVHRWMVRSD
                     LKDDLPSFIDGYGNVVHCHSINQPHLAAAIFVEGEVETMNTHGIVRGAPEPLPPLFFL
                     RRTPLTMPDAALEALAGSVARQGSVLKQLHHLVRLVRDKVEYRPGATDEATSAAAALA
                     AGVGVCQDHAHIFISAARLLGIPARYVGGYLWTSDDVREYQATHAWAEAFVHDLGWVG
                     FDPSNRICPTEAYIRTSVGLDYASAALVRGGRRGSAAETLAVKVKITQAGSDQ"
     gene            complement(6317..7261)
                     /locus_tag="VGP50_11095"
     CDS             complement(6317..7261)
                     /locus_tag="VGP50_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEV8027969.1"
                     /translation="MLSRTADSLFWLARYMERAENVARIMMVGHRMASMARSLENPGN
                     EWRSTLIAAGCEEGFFAKYTEPEAAAVSDYLVRDLDNPSSIFSCLETARRNARAVRTA
                     LTVDMWDALNETWLKTRSPDAGAYAPERIMALLEWVKERSLLFNGAYANTMLRNDAYY
                     FTRLGTFIERADNTARILDVKYHVLLPRHEGVGGALDYYHWQAILRSVSALRSYHWVY
                     HDRLKPWLVAELLILRPEMPRSLLSCCEQIVRYLDLLADAYGGLRGECHRIAGEMHAR
                     LRQGRVQDIFQSGLHEFLTEFIGRSVVLGGEISRLYLL"
     gene            complement(7264..8658)
                     /locus_tag="VGP50_11100"
     CDS             complement(7264..8658)
                     /locus_tag="VGP50_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEV8027970.1"
                     /translation="MGAGEARRSHYELIHRWLEETPAEALSLKRREAELLFRRIGITF
                     AVYAEGGDPERLIPFDIIPRVLDWAEWTVLARGLTQRVRALNAFIADVYHRRQILRAG
                     HIPPELVLHNEGFRPEMQDFEPAQGVYTHIAGIDIVRLGPRDFYVLEDNCRTPSGVSY
                     MIENREAMMRLFPDLVARHRIAPVAHYPEDLLDTLKSVAPANCEDEPVVVLMTPGSHN
                     SAYYEHSFLADEMGIELVEGADLFIEDNIVYMRTTEGPRRVDVIYRRVDDDFIDPLTF
                     RPESVLGTPGLFNAYRAGNVTLANGVGAGIADDKSVYRFVPQMIRFYLGEEPILNNVP
                     TYCCSKREDLDYVLEHLGELVVKEARGSGGYGMLIGPQATREQLAAFSDKIRAKPGDY
                     IAQPTLALSTCPTFVETGVAPRHVDLRPFVLCGKEIRIVPGGLTRVALREGSLVVNSS
                     QGGGTKDTWVLENP"
     gene            8796..9320
                     /locus_tag="VGP50_11105"
     CDS             8796..9320
                     /locus_tag="VGP50_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027971.1"
                     /translation="MTRLDFATADAAGAGRSQRRLPKKWANPSEVDRRPRNIPRMPRG
                     GFDRRQRDMLAPRAGDDWTPTGSPALLVVDSVGGSPHQVERPLAAHLLLASTRSASRA
                     PCLRHLASIDGDVTAASGETESTLNSRREDSGRHIAFRRRKARRSNVIYFTRSITRMD
                     YTLAHLPSFWLKSS"
     gene            9390..11147
                     /locus_tag="VGP50_11110"
     CDS             9390..11147
                     /locus_tag="VGP50_11110"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEV8027972.1"
                     /translation="MFVARAFRETRSVRALVSGGGTALFLTFAVTGTTFAQAVAQGAG
                     QVAPPTSPIERAVPVPTPQAVPPLLPTPAPGAPEVPPGPPVAIQSVVVQGATAYPESE
                     LKPYYAGLVGQSVPLAQISSVLQQIQSKYRNDGYVLTVVRGAIEPGTGGSTLRIRVVE
                     GFISDVKIDGDIGPAGVLVFNFLNKLTTIRPVNISDIERALLLAQDIPGVSVRAVLRP
                     GTGEAGAVELVGQVGRKAFGGYVQYDNRASNFAGPNELLVGAQANSFTSAGERTEVEI
                     YDTPFTTEQLFGQGSIEGFVGSSGLKLRGYAGYGYSQPGGVLSAVDFHSRLTLAGVSA
                     SYPIIRTRPLSLYTSLAFDTEHAVTTESGGTGLRNVSDLRVLRLSSTVDSQDQTLGTG
                     LIGANTIILTAHEGLNGLGATSDARIGNRVDFFKLTGEITRVQNLFSFGTNTVALKLS
                     AGGQWTNDVLPPTEEYLLGGTRYGRGFFAGEVIGDNALGATAELQLNTAIERPLPLGL
                     QPYIFYDNGQTWNVAPAGQPPDPSQRIESAGVGVRVTVTPQLSVDVEDAHRFTRTPTG
                     AAVSREGADVIFVTVIARF"
     gene            11233..>15192
                     /locus_tag="VGP50_11115"
     CDS             11233..>15192
                     /locus_tag="VGP50_11115"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEV8027973.1"
                     /translation="MQKPETRLSAPLALNVPHAARRASAKGITRQLRASTALRLLFRA
                     TAWTGVSISIISSATANPTGGKVVQGQATINQSDPNATVITQTTQNATINWNSFGIDA
                     GQRTSIYVPNKSAVEVERVTGGDPSKISGLLESRVGTPGAPGAYGGTIVLINPNGIVF
                     SGGSQVNVGGLIASTADLAANLRDNDPVQRLNFNKPSPNPNATIVNQGTITVADHGLA
                     ALVAPGVQNSGVITAKFGKVMLAGAQTFTVDLYGDGLLSFDVTSKIKGAIAINDGQIN
                     APGGVIELSASAIDDVVAGVINIDGKPVVNGVYVGGTVSADAQGAAGGTIIADAGSGH
                     LSVSGQISASGVNPGETGGSIALTGNSVEVQSGATIDASGSAGGGSIKVGGGFPGTDT
                     SIRNAKTTTIAAGATLDASALDKGNGGSVVVWSDQKTDFAGTIVAKGGAQGGDGGKAE
                     VSSKDVLNFTGFADLRAPFGKAGTLLLDPLELDICATTCTATTSDTQSDLSVSMLLAE
                     LGSGTNVQLSADVIDFQSSIPYTGATAASLSATATQSITLESNVSISSTAAPLAVNLN
                     AGPTGSVTFNSGSSILTDGGQLSVAAGTGGLSFADNTVQLNTTGTLSDGNLSLSAPSL
                     LLTGTINAGAATLSLTASAGDITENAATVTAGTLTGSSGGSLTLAGAGGSTNSIGDLN
                     TFTVGTSGNFSLADATALTIDGTVTVPGTFTLNAGGAISETGAATINAGTLTGSSVGT
                     ADLTATNTIADLNTFTVNGSGDLAINDLGQTGALTIDGTVTVPGTFNIAAGGTISETG
                     SINAATLQGRSAGTANLSGNNTIADLNRFSVTSGDLVLNDLGDTGTLTIDGAVTVPGT
                     FTLNAGGAISETGAATINAGTLTGSSVGTANLTTTNTIADLNTFTVNGSGDLAINDLG
                     QTGALTIDGTVTVPGTFNIAAGGTISETGSISAATLKGRSGGTAALNGTNTIADLNTF
                     TVTSGNFSLTDATALTIDGAVAAPGTVNLAIGGTITEETGGSISAGTLSGSSVGTTSL
                     AQTNTIADLGSFTVTSSGDFVLADSTALAINGAVAAPGTVNLAVNGTITEETGGSISA
                     ATLTGSSVGTTSLAQTNSIADLNTFTVNSTGDFVLADATSLSIDGNVATNNGNIALTI
                     GGTSLSITGTVNSGAGTTTITAADEALAAGDGVSGGLAISDGSLGHIIAGGGLTLATL
                     GNNDITVGTVTGHAGFSQLTFNSGQDITLDGPLTVATGGNGTLRLIAAGAITENSGGT
                     ITAGTLTGSSGGSTTLGQTNTIADLNTFTVGGDLALNDLGETALTIDGTVTVPGVFTV
                     "
BASE COUNT         2639 a         5222 c         4867 g         2464 t
ORIGIN      
        1 ggcggcccac cggcaagccc accatcttcg ccgcgcggac attgccgccg accacccgcg
       61 cggcgaggcc gaaggtcgtc cgataaagga agacataggc gagtaccgcc gccaccagcc
      121 cgaacaccag gccccaatgc acctcagtgc cggggatcat gccgatcatg ttggcgtcgc
      181 cgatgccggg cgatgacggc ttgttgaggc tcgccggatc gcgcatcggt ccttcgacaa
      241 gatggttgag cagcgcgatc gcgatataga cgaggagcag gctcgagatg gtctcgttga
      301 cgccgcgata gtgccgcaac gcgccgacaa gggcgatcca cgcgccgccc gcggccatcc
      361 cggcggctgc catggcgagc tgcacgccga gcggtgccat cccttgaagc gagagagcgg
      421 ccgcgacggc cgcgaggccg ccgaccacca gcgccccctc gccgccgatc accacgagac
      481 cgagctgcgc cgggagcgcc gtgcagagcg ccgtcaggac caggggcgcg gcgcggatga
      541 ggctgttctg ccacgagaac caggagccga acgcgccctt gtacatgagg ccataggcgc
      601 tgagcggatt gacgcggcag agggcgatga agacgccgaa caccagcaag gccgccgcga
      661 gcgccgcgag cggtatggcg acgctctctg cggcggcaag aaggcgcgga tcgatcgaca
      721 ggcgaagcgg cggacgcgcg atctccgcta tccggccatt gtccctcatg cggccgagcc
      781 gatcactccg tcgacgagcc agttcatctg ctcgagccag aggtcggtct ggccgtgcgc
      841 cgtgccttcg gcaatgacgg cgttgccttt gttgtccttg atcggtcctt tgaagatgac
      901 gtagtcgccg gccatgaact tcgccttgac ggcatcggca tgctgacgcg ctttgtcacc
      961 gaccaacggg ccataggccg aggtcttcac cagcccttcc ttgagcccgc cgcgatacat
     1021 gttcggaatg gtctcgcccg ccatgaacat cttgatgaag cgcgggtaga gctcttccca
     1081 attccactcg gcgccggtga gatagccttt gggcgcgagc gtcgactggt tggcgtgata
     1141 gccgccgacc atgatgccgc ggcgctccgc cgtctcgacg accaccttcg ggctgtcgac
     1201 atggcaggtg acgacgtcga tgccctggtt cgccaggctg ttggtcgcct ccgcctcttt
     1261 gatcggcagc gcccagtcgc cggtgaagat gacttgcgtg gtgatatccg gattgaccat
     1321 ccgcgccccc agggtgaagg cgttgatgtt gcgcagcacc tgcgggatcg gtttcgccgc
     1381 gacgaagccg agcttgcccg ttttgctggt gtatccggca acgatgccgg agagatgctg
     1441 gcactcgtcg atatagccga aatagctgcc ggcgttcgtc ggatccttct cgctccagag
     1501 gccgccgcaa tgctcgaaac gaacattcgg agtcttcgcc gctatcttca ccatgtacgg
     1561 gttgtagtag ccgaaggagg tggggaagag cagcgtcgcg ccgtcgaggt tgatcatcga
     1621 ctccatggtc ttctcgacag cgaccgtctc cggcacattc tcttcctcga gcacgctgac
     1681 tccggccatc ttcttcaccg cggccgcgcc ctgcgcatgc gcctggttat agccatagtc
     1741 gtcgcgcggc ccgacataga tgaagccgac cacgaggttg gcggccaagg cgcggcgggc
     1801 ggggaggagg cccgccagcg tccccgccgc gcccaacgcg ctggcgccca gcaaaagatc
     1861 gcgacgcgag atcctgtatt ccatcggccc cctccgtctt tggtctggtc tcgtcggctc
     1921 ggatgtcgca aaccccatgc cagccgcatt gcacgatccg atccgttatc gcgacgtgac
     1981 gattgccgag tcctgctcat aaaacaggca tcaatgcgct tgttgtatgc ggtcgcctgg
     2041 cgctgcggca gcccgcgcct agaatggcgt tggcaccgac tctgcagagc cgacgggaaa
     2101 ggagcgggag atggcacgga atcgcccaag cggagcgcca agcctcgatc tgatccgcct
     2161 aagggaacac ttcgagcggg gcgggctcac ggtcgcggcg ctgatcgagg agatcatcgc
     2221 gcggcttacg gctgccggcg atgacaaggt gtggatctcc cgcgcttcgg acgacgcgtt
     2281 gcgcgtgcgt gcggctgctc tcgacgcgcg gcgcgccgtc gaccctcagc tgcttgcgcg
     2341 gctgccgctc ttcggcgtgc ccttcggggt caaggacaac atcgacgccg cgggcttcgc
     2401 gacgacggcc gcttgtccgg ccttctctta tctgccggcc gcgtcggcac cgtcggtcgc
     2461 acgcctgctc gaggccggcg cggtgctggt tggcaagacc aatctcgatc agttcgccac
     2521 gggcctggtc ggcacgcgct cgccctatgg ggtgccgcgc aatcccttcg acgaccgcta
     2581 tattcccggc ggatcgagct ccggctcggc cgtcgccgta gccaaaggcc tcgtcagctt
     2641 cgccctcggc accgatacgg ccggatcggg ccgcgtgccc gccgccttca acaacatcgt
     2701 cggcctgaag ccgagccgcg gcgtgatcag tgcgcgcggc gtcgtgccgg cctgccgcac
     2761 gctcgattgc gtctcgatct tcgcgctcac cgctgacgac gcgcagcgga ttctcgccat
     2821 ctgcgccggc tacgacgagg ccgaccctta ttcacggccg atgccggtgc ccgcgccggc
     2881 tttcggacgt ccgttccgct tcgcggtgcc gcgcgcgcag gatcgcgaat tcttcggcga
     2941 tgcggagaac gcgctgctct tcgagcaggc gctctcggcg ctcgaagctt tgggcggcac
     3001 gcccgtgccg gccgattttt cgcctttccg cgccgcgggc gcgcttcttt atggcggccc
     3061 ctgggtcgcc gagcggctcg aggcgagcga ggcgctgctg cgccgcgatc ccgatgccgt
     3121 cctgcccgtc acgcgcgaga tcatcgccgg cggcggaacc tatggcgcgg tagacgtctt
     3181 tcgcgcgcag tatcgcctgg ccgagctgcg gcgcgaaagc gaggcggtct ggcgcgaagc
     3241 cgatctgctg gtcgtgccga cggccgcgac gatctacacg ctggcggaga tcgcggccga
     3301 tcccatcggc cgcaacgcca atctcggccg ctacaccacc ttcaccaatc tgctcgacct
     3361 cgccgccgtc gctgtccctg cggggttccg ccgcgatggc ctgcccttcg gcctcacctt
     3421 cctcgctccc gccttcagcg atctcgcttt gtgcgccatc gcccaagcgt atcagcacag
     3481 cgtcgccttg ccgctcggcg ccacgccgtt gcgtctaccg ccgccgtccg cggcgagcgc
     3541 caggccggag accgacggcc tcattcgtgt ggccgtcgcc ggggcgcatc tgacgggaat
     3601 gccgctcaac ggcaagctgc tggcgctcgg cgcgacctgc gtcgctgccg ccgagacggc
     3661 gccggtctat cgcatgtttc ttctgcccca ggatccgccg cgcccggggc tcgtgcgcgt
     3721 cggcgagggc ggtcgcggct tcgcgatcga gatctgggcc atgagcgtcg ccgccttcgg
     3781 ccgcttcgtc gatgacgtgg ccgagccttt ggcgatcggc acagtgctgc tcgcagatgg
     3841 cagcaaggta aaaggctttc tttgcgaagc aagcgccgcc gccgaggccg aggacatctc
     3901 gcgcttcggc agctggcgga gcttcatcgc gacgagggag tgagcggagg acgcgccccg
     3961 cgccgaaaac ctcgatgtca cgctccgccc gccctcacga gacggatgcg ctactccgcc
     4021 gcgtcggcgg gccgtagcga cgcggcgtat tcggcaaaag agcggcgcac gcccgcgtga
     4081 tggcggcgca tggtttggaa agcctgctcg ccgtcgccac gcaggatggc gcgcacgacc
     4141 tgatcatgct cggcatagga ggcggcgaga cggccggcgc tgcggaattg gctccggctc
     4201 agcggcgcga aacggccgcg tagcgtccct acgatctcct gcagcaccga gttttgaccg
     4261 ccctggcgaa tggcggcatg aaaatcggca ttggcggcgt ggtagagctc agtatcgccg
     4321 ctctgcacca gccgcccgca ttggcggtgg agctcttcga gtcgatagcg ctcgggcgcc
     4381 gacatcttga gtgccgaaag ccgcgcgcag accgcctcca gctcggccac cgcctcgaaa
     4441 atgtcgtgga accgcgcctc cgtcatcggc gtgacgacgg cacccacatg cgcccggctc
     4501 tcggcgaggc cgctcgccgc gagttcgcgc agcgcctcgc gcaccggcgt gcgcgacacc
     4561 gcaaagcgct tggcgagcga cgtctcgtcg aggcgggtgc cggcgcgcag gcgccccgcg
     4621 atgatgtcgt cggcaagcgt atcgcgcacc agatcggccc gccgccgcgg cgctttcccg
     4681 ccggcggcgg ccttcgatgg agcctcgttg atcatggttt cgctgcgaac gcccaatcgg
     4741 gccgcggaat ggcgcggtag gcctcccgca gcgcctgcga ccagcgctcg cggatcatgg
     4801 cgaaataggg atcgtcctcg gtgatgcggc ggcgcagctc gaccctgagc gcgtcgcggc
     4861 gatagaccag gagatcgatg ggcagcccga cggtgagatt gctgcgcagg gtcgaatcca
     4921 tcgacaccag cgccagcttc acgccgtcat agaggtcggt atcgtagcgc gccgcgcgat
     4981 cgaggatggg cttgccgtat ttatgctcgc ccacctgcag aaagggagtg tcgggcgtcg
     5041 cttcgatgaa attccccgcg gcgtagacct ggaagaggcg cagcgagcgc cccttgatct
     5101 ggccacccag caggaaggat atgtcgaaac tcacgttctg cgcctgcatc gcagcaccgt
     5161 cgatgtggta gatccggcgc accgcctcgc cgacgagctg cgccgcgcgg aacatgctct
     5221 tgaccgtcag cagcgtctcc ggcgcaccgt cgcggtcgat accctcgtgc agcagattga
     5281 cgaccgactg cgcgatcgcg agattgccgg cggcgagcag cacgatcacc cgctcgcccg
     5341 gcacctcggt gacatgcatc ttcgagaagg tcgagatgtt gtcgaccccg gcattggtgc
     5401 gcgtgtccga gagcagcacc aagccctcct tgagatcgag cccgacacaa taagtcatcg
     5461 cagcatttcc tcgccggaac atttattgat cgctgccggc ttgcgtgatc ttcaccttga
     5521 ccgccagcgt ctcggcagcg ctgccgcggc gcccgccgcg caccagtgcc gccgaggcat
     5581 agtcgaggcc gacgctcgtg cggatatagg cttccgtcgg gcagatccgg ttcgacggat
     5641 cgaagccgac ccagcccaga tcatgcacga aggcctcggc ccaggcatgc gtcgcttgat
     5701 actcgcgcac atcgtccgat gtccacaaat atccgcccac ataccgcgcg ggaatgccga
     5761 ggagccgcgc cgccgaaatg aagatatgcg cgtgatcctg gcagacgccg acgccggccg
     5821 ccaacgcggc ggccgcggag gtggcctcgt ccgtggcgcc cggccgatac tcgaccttgt
     5881 cgcgcaccag cctcacgaga tgatggagct gcttcaggac cgagccctga cgcgcgacgg
     5941 aacccgccag cgcctcgagc gcggcatcgg gcatggtgag cggcgtgcgg cgcaggaaga
     6001 agagcggcgg cagcggctcc ggtgcgccgc ggacgatgcc atgagtgttc atggtttcca
     6061 cttcgccttc gacgaagatt gctgcggcga gatgcggctg gttgatcgaa tggcaatgga
     6121 cgacgttgcc gtaaccgtcg atgaagctcg gcagatcgtc cttcaagtcg ctgcgcacca
     6181 tccagcgatg cacggtcagc ccctcgtatg agcggggtat caggcgcagc gtctgcgtcg
     6241 ccgcggcgac cggcgtctcg tagcgataag ccgtctgatg tcggacctgg aggcgcatcg
     6301 gttcggccgg gatctcttac aggagataaa gccggctgat ctcgccgccc agcaccaccg
     6361 agcgaccgat gaactcggtg aggaattcgt ggaggcccga ctgaaagatg tcctgaaccc
     6421 ggccctggcg cagccgcgca tgcatctctc cggcgatgcg atggcattcg ccgcgcaacc
     6481 cgccataggc gtcggccagc aggtcgagat aacgcacgat ctgctcgcag cagctgagca
     6541 gcgagcgggg catctccggc cgcaggatga gcagctcggc aacgagccag ggcttcaggc
     6601 gatcgtgata gacccagtgg tagctgcgca gcgccgagac cgagcgcagg atcgcctgcc
     6661 agtgataata atcgagcgcg ccgcccacac cctcatgacg cggcagcagc acatgatatt
     6721 tcacatcgag gatgcgcgcc gtgttgtcgg cccgctcgat gaacgtgccg aggcgcgtga
     6781 agtaataggc gtcgttgcgc agcatggtgt tggcataggc gccattgaac agcagggacc
     6841 gctccttcac ccattcgagg agcgccatga tgcgctcggg cgcataggcg ccggcatcgg
     6901 ggctgcgcgt cttgagccag gtctcgttga gcgcgtccca catgtcgacg gtgagcgcgg
     6961 tgcgcacggc gcgcgcattg cgccgcgccg tctcgaggca ggagaagatg ctcgagggat
     7021 tgtcgaggtc gcgcaccaga tagtcgctga ccgccgccgc ctcgggctcg gtgtatttgg
     7081 cgaagaaccc ttcctcgcag cccgccgcaa tgagcgtcga gcgccattcg ttgccgggat
     7141 tctcgagcga gcgggccatg ctcgccatgc gatggccgac catcatgatg cgcgcgacat
     7201 tctccgcgcg ttccatgtag cgcgccagcc agaagagact gtcggccgtg cggctcagca
     7261 tcgctaggga ttctccagta cccaggtgtc cttcgttccg ccgccctggc tcgaattgac
     7321 gacgagcgag ccttcgcgca gcgccacgcg ggtgaggccg ccggggacga tgcggatctc
     7381 cttgccgcaa agcacgaagg gacggagatc gacatggcgc ggggcgacgc cggtctcgac
     7441 gaaagtcggg caggtcgaga gcgcgagcgt cggttgcgcg atgtaatcgc ccggcttcgc
     7501 gcggatcttg tcggagaagg cggcgagctg ctcgcgcgtc gcctgcgggc cgatcagcat
     7561 gccgtagccg ccggagccgc gcgcctcctt caccacgagc tcgcccagat gttccagcac
     7621 gtagtcgaga tcctcgcgct tcgagcagca ataagtcggc acgttgttga ggatcggctc
     7681 ctcgcccaga taaaagcgga tcatctgggg cacgaagcga tagaccgact tgtcgtcggc
     7741 gatcccggcg ccgacgccgt tggcgagcgt gacatttccg gcgcgataag cgttgaagag
     7801 gccgggcgtt ccgagcaccg attccggccg gaaggtcaag ggatcgatga agtcgtcatc
     7861 gacgcgccgg tagatcacgt cgacgcggcg cggcccctcg gtcgtgcgca tgtagacgat
     7921 attgtcctcg atgaagagat cggcgccttc caccagctcg attcccatct cgtcagcgag
     7981 gaaggaatgc tcgtaatagg cactgttgtg ggagccgggc gtcatcagca cgacgaccgg
     8041 ctcgtcctcg caattggccg gggccaccga tttcagcgta tcgagcagat cctcgggata
     8101 gtgggcgacg ggcgcgatgc gatggcgcgc gacgaggtcg ggaaagagcc gcatcatggc
     8161 ctcccggttc tcgatcatgt aggagacgcc cgaaggtgtg cggcaattgt cctcgagcac
     8221 gtagaagtcg cgcggaccga ggcgcacgat gtcgatgccg gcgatgtggg tgtagacgcc
     8281 ttgcgccggc tcgaaatcct gcatctccgg acggaagccc tcgttgtgga ggacgagttc
     8341 cggcgggatg tgtccggcgc gcaggatctg ccggcgatga tagacatcgg caatgaaggc
     8401 gttgagcgcg cggacgcgct gcgtcagtcc gcgcgccagg accgtccact cggcccagtc
     8461 gaggacgcgc gggatgatgt cgaagggaat gaggcgctcg ggatcgccgc cttcggcata
     8521 gacggcaaag gtgatgccga tccgccggaa cagcagctcg gcctcgcgcc gcttcagcga
     8581 caatgcttcc gccggtgtct cctcgagcca gcggtggatc agctcgtaat gtgatcggcg
     8641 cgcctcgcct gcgcccatct cgtcgaaggc ggtggaggcc ctgactactg gtgtattcgt
     8701 cacaagagct cccgctgctc cgcgagcgag gaccgctccg cttccaagtc tttccctgca
     8761 agcgatgcgc cagtcgggcc gagccgcgca agcgcttgac ccggctggac ttcgcgaccg
     8821 ctgacgccgc cggcgccggt cgttcccaac ggcggctgcc gaaaaaatgg gcaaatccct
     8881 cggaagttga tcggcgtccg cggaacatcc cgcgcatgcc gcgcggcggc ttcgatcgcc
     8941 gccagcgcga catgctcgcg ccacgggcgg gcgatgactg gacgccgacc ggaagtccgg
     9001 cgctgctcgt tgtcgacagt gtaggcggct cgccgcacca ggtcgagcga ccgctcgcgg
     9061 ctcatctcct cctcgcctcg acgcgctctg catcgcgcgc gccatgctta cgccatttgg
     9121 caagtatcga cggcgacgtc acggcagcat caggcgagac ggagtccacc ctgaacagta
     9181 gaagagaaga ttcgggacgc cacatcgcat ttcgccggcg aaaagctcgg cgaagcaatg
     9241 tgatctattt cacacggtcg atcaccagaa tggattatac gcttgcgcat cttccctcat
     9301 tttggctaaa aagtagctag aaattagcga caatttaaac cattcggggg aatgggcggt
     9361 tcgatctcac acccgtgggc gtccgaagaa tgttcgttgc ccgagctttc cgtgaaacac
     9421 gcagtgtgcg cgccctcgtc tccggcggtg gcactgcgct gtttctgacg ttcgccgtca
     9481 cgggaaccac ctttgcccaa gccgtcgcgc aaggcgcggg gcaggtggcg ccgccgacgt
     9541 ccccgatcga gcgcgccgtg cccgtgccga cgccccaggc agtgccgccg ctgttgccga
     9601 ccccggcgcc gggcgcgccg gaagtgcccc ccggaccgcc ggtggcgatc cagtcggtcg
     9661 tggtccaagg agcgacggcc tatcccgaga gcgagctcaa gccctactac gccggcctcg
     9721 tcggtcagag cgtgccgctc gcccaaatct cgtccgtcct gcagcagatc cagagcaagt
     9781 atcgcaatga cggctatgtg ctgacggtgg tgcgcggcgc catcgagccg gggaccggcg
     9841 gctcgacgct gcgcatccgc gtcgtcgagg gcttcatcag cgacgtcaag atcgacggcg
     9901 acatcggtcc tgcgggcgtt ctggtcttca acttcctcaa caagctcacg accatccgcc
     9961 cggtcaacat ctcggacatc gagcgcgcgc tcctgctggc gcaggacatt ccgggcgtct
    10021 cggtgcgcgc cgtgctgcgt cccgggaccg gcgaggcggg tgcggtggag ctggtcgggc
    10081 aggtcggccg caaggcgttc ggcggctatg tgcaatatga caatcgcgcg tcaaacttcg
    10141 ccggccccaa tgaattgctg gtcggtgccc aggccaactc cttcaccagt gccggcgagc
    10201 gcaccgaagt tgaaatctac gacacgccct ttaccaccga gcagcttttc ggccagggct
    10261 cgatcgaagg cttcgtcgga tcgagtgggc tcaagctgcg cggctatgcc ggctatggct
    10321 actcgcagcc gggcggcgtg ctgtccgcgg tcgatttcca ttcccggctg acgctcgccg
    10381 gcgtatcggc ctcctacccg atcatccgca cgcggccgct ctctctttat acgtccctcg
    10441 ccttcgacac cgagcacgcg gtaaccacag aatcgggcgg cacgggtttg cgcaacgtca
    10501 gcgatctgcg cgtgctgcgc ctgagcagca cggtcgactc gcaggaccag acgctcggca
    10561 ccggccttat cggcgccaac accataatcc tgacggcgca tgaaggactg aacggtctcg
    10621 gcgccaccag cgatgcgcgg atcggcaacc gcgtcgattt cttcaagctg acgggcgaga
    10681 tcacgcgcgt tcagaacctt ttcagtttcg gcaccaacac ggttgctttg aagctgtccg
    10741 ccggcggtca atggaccaac gacgtcctgc cgccgaccga agaatacctc ttgggcggca
    10801 cgcgctatgg gcgcggtttc ttcgccggcg aagtgatcgg cgacaatgcg ctcggcgcga
    10861 cggcggagct gcagctcaac acggccatcg agcggccgct cccgctcggg cttcagcctt
    10921 acatctttta cgataacggt cagacgtgga atgtggcgcc ggcgggccaa ccgccggacc
    10981 ccagccagcg catcgaatct gctggcgtcg gggtccgagt gacggtgacg ccccagcttt
    11041 cggtcgatgt cgaggacgcc catcgcttca cgcggacgcc gaccggcgcc gcggtgtcgc
    11101 gtgagggggc ggatgtcatc ttcgtcacgg tgattgcaag gttctagtcg ctcggttccg
    11161 agtcgttcgt cgaatttctg tcgaagagtc cgagtcaatc ggaccgtcag gcagaggagg
    11221 caaaggaagc acatgcaaaa gcccgaaacg cgtctttcgg cgccgttggc gctgaacgtc
    11281 ccgcacgccg cccgccgcgc atccgccaag ggcatcacgc gtcagttgcg cgcctcgacg
    11341 gcgctgcgcc tattgttccg cgcgacggcc tggaccggcg tctccatcag catcatttcc
    11401 tcggcgacgg ccaatccgac cggcggcaaa gtcgttcagg gacaagcgac catcaaccaa
    11461 agcgacccga acgcgacggt catcacacag acgacgcaaa acgccacgat caactggaac
    11521 agcttcggca tcgacgccgg ccagcgcacc tcgatctacg taccgaacaa gagcgcggtc
    11581 gaagtcgaac gggtgaccgg cggcgatccc tcgaagatca gcggccttct cgaatcgcga
    11641 gttggcaccc cgggcgcgcc cggcgcttat ggcggcacga tcgtcctcat caacccgaac
    11701 ggcatcgtgt tctcgggcgg ctcgcaggtc aacgtcggcg gcctcatcgc ctcgacggcg
    11761 gacctcgccg ccaacctccg cgacaacgat cccgtccagc gcctgaattt caacaagccg
    11821 tcgcccaacc cgaacgccac aatcgtcaat cagggcacca tcaccgtcgc cgatcatggc
    11881 ctcgccgctc tcgtcgcgcc gggcgtgcag aactccggcg tcatcactgc gaaatttggc
    11941 aaggtgatgc tggccggcgc ccaaaccttc accgtcgatc tctatggcga cggtctcttg
    12001 tccttcgacg tcaccagcaa gatcaagggc gccatcgcga tcaacgatgg acagatcaac
    12061 gccccgggcg gcgtcattga gctttcggca agcgccatcg acgatgtcgt ggccggcgtc
    12121 atcaacatcg acggcaagcc ggtggtgaac ggcgtctatg tcggcggcac ggtgagcgcc
    12181 gatgcgcagg gcgcggcggg cggcaccatc atcgccgatg ccggcagcgg ccatctcagc
    12241 gtgtcgggcc aaatttcggc gagcggcgta aatcccggcg agaccggcgg cagcatcgcg
    12301 ctcaccggca acagcgtcga ggtgcaaagc ggcgcgacca tcgacgcgag cggcagcgcg
    12361 ggcggcggca gcatcaaggt cggcggcggc ttccctggga cggatacgag catccgcaac
    12421 gcgaagacga cgaccatcgc cgcgggcgcc acgctcgacg cgagcgcgct cgacaagggc
    12481 aatggcggct ccgtcgtcgt gtggtcggac cagaagaccg atttcgccgg aacgatcgtc
    12541 gccaaaggcg gcgctcaggg tggcgacggc ggcaaagccg aggtctcgag caaggatgta
    12601 ctgaacttca ccggatttgc cgatctgcgc gcgccgtttg gcaaagcggg aacgctgttg
    12661 ctcgatccgc tcgagctcga catatgcgcg acgacctgca cggcaaccac tagcgatacg
    12721 cagtcagatc tatccgtcag catgcttctc gcagaactcg gcagcggcac gaacgtgcag
    12781 ctgagcgccg atgtcatcga cttccaaagc agcattccct acaccggcgc gacagccgca
    12841 agcctttccg ctaccgcaac acagtccatc acgcttgaat cgaatgtctc gataagctcg
    12901 accgccgcgc ctctggcggt gaatctaaac gccgggccga cgggcagcgt tacatttaac
    12961 tcaggctcct cgatcctgac ggatggtggc cagctgtcgg tcgccgctgg tacgggcggc
    13021 ctctcgtttg cggacaatac ggttcagcta aatacgacgg gcaccctctc ggacggcaac
    13081 ctgtcgcttt ccgccccgag cctgttgctc accggcacca taaacgccgg tgccgccacg
    13141 ctgtcactca cggcttcggc aggagacatt acggagaacg ccgccaccgt cacggcaggc
    13201 acgctaacgg gctcgtccgg cggttcgctg acattggccg gcgccggcgg cagcaccaac
    13261 agcatcggcg acctcaacac ctttacggtc ggcaccagcg ggaatttcag cctcgccgac
    13321 gcgacggccc tgacgatcga cggcacggtc accgtgcccg gcaccttcac gctcaatgcc
    13381 ggcggcgcga tcagcgagac cggcgccgcc acgatcaatg ccggcacctt aaccggctcg
    13441 tcggtcggca ccgccgatct caccgcgacc aacaccatcg ccgacctcaa cacctttacc
    13501 gtcaacggca gcggcgacct cgccatcaac gatctcggcc agaccggggc gctcaccatc
    13561 gacggcacgg tcaccgtgcc gggcacgttc aacatcgccg ccggcggcac catcagcgag
    13621 accggctcga tcaacgccgc cacgcttcag ggccgctcgg ccggcacggc gaatctcagc
    13681 ggcaacaaca cgatcgccga cctcaaccgc ttcagcgtca ccagcggcga cctcgtcctc
    13741 aacgacctcg gtgatacggg aacactcacc atcgacggcg cggtcaccgt gcccggcacc
    13801 ttcacgctca atgccggcgg cgcgatcagc gagaccggcg ccgccacgat caatgccggc
    13861 accttgaccg gctcttcggt cggcaccgcg aatctgacca cgaccaacac gatcgccgac
    13921 ctcaacacct ttaccgtcaa cggcagcggc gacctcgcca tcaacgatct cggccagacc
    13981 ggggcgctca ccatcgacgg cacggtcacc gtgccaggca cgttcaacat cgccgccggc
    14041 ggcaccatca gcgagaccgg ttcgatcagc gccgccacgc ttaagggccg ctcgggcggc
    14101 accgccgcgc tcaacggcac caacaccatt gccgacctca acaccttcac tgtcaccagc
    14161 ggcaatttca gcctcaccga cgcgacggcc ctgacgatcg acggcgccgt cgcggcgccg
    14221 ggcacggtga acctcgccat cggcggcacc atcaccgaag agaccggcgg ctcgatcagt
    14281 gccggaaccc tcagcggctc ctcggtcggc accaccagcc tcgcgcagac caacaccatc
    14341 gccgatctcg gcagtttcac cgtcaccagc agcggcgact tcgtcctcgc cgacagcacg
    14401 gcgctcgcca tcaacggcgc cgtcgcggcg ccgggcacgg tcaacctcgc cgtcaacggg
    14461 accatcaccg aagagaccgg cggctcgatc agcgccgcca ccctcacggg atcttcggtc
    14521 ggcacgacca gcctcgctca gaccaacagc atcgccgacc tcaacacctt caccgtcaac
    14581 agcacgggcg acttcgtcct ggccgacgcc acgtcgctct cgatcgacgg caatgtcgcg
    14641 accaacaacg gcaacatcgc cctgacgatc ggcggtacgt cgctgtcgat caccgggacc
    14701 gtcaacagcg gcgccggaac gacgacgatt actgcggccg acgaagcact ggccgcgggc
    14761 gacggcgtga gcggcggcct cgccatcagc gacggctcgc tcggtcacat catcgcgggc
    14821 ggcgggttga ccctggcgac gctggggaac aacgacatca ccgtcggcac ggtgaccggc
    14881 catgccggct tcagccagct cactttcaat tccggccaag acatcaccct ggacggcccg
    14941 ctgacggtcg cgacgggcgg caacgggacc ttgcgcctga tcgccgccgg cgcgatcacc
    15001 gagaattccg gcggcacgat cacggccggg acgcttaccg gctcctcggg tggcagcacc
    15061 acgctcggcc agaccaacac catcgccgat ctcaacacct tcaccgtcgg cggcgacctc
    15121 gccctcaacg atctcggcga gacggcgctc accatcgacg gcaccgtcac ggtgccgggc
    15181 gtcttcaccg tc
//
LOCUS       DASYNS010000356         3501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4612494, whole genome shotgun sequence.
ACCESSION   DASYNS010000356 DASYNS010000000
VERSION     DASYNS010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4612494"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VGP50_11120"
     CDS             complement(<1..500)
                     /locus_tag="VGP50_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027974.1"
                     /translation="MARGPLLWGACGGGLAGVIVLVGLVWLRSADPQEQSRAPDRGLA
                     PTNAAAALPAGAKPGIEDMPCWFAVPAGRSARCGSLAVPERWTGSPSRLLHLRFVVFR
                     GDPGGAEDPVIYLAGGPGEPAQIDAESIGHWWEWIARAPWLRTRDLVVFDSRGVGLSE
                     PKMGCP"
     gene            complement(571..1158)
                     /locus_tag="VGP50_11125"
     CDS             complement(571..1158)
                     /locus_tag="VGP50_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV8027975.1"
                     /translation="MRGYLFPTVIAFAFTAGMTIAQAEDAKPADNAPASDQKPADKNV
                     SSDQLIKELTPPPGTPPLRFRGLHVLNSQPAGAGSTEMPAVGLDIKFKLGSAELTDDA
                     KDQVRQLAEAMNSDQLSKYHFLVEGHTDSTGQPDRNLALSKRRAEAVRSYLVSAYNVP
                     RGRLEAVGRGQTKPINASNPADPANRRVQVVNLGQ"
     gene            complement(1323..3269)
                     /locus_tag="VGP50_11130"
     CDS             complement(1323..3269)
                     /locus_tag="VGP50_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV8027976.1"
                     /translation="MADDRTRVPGTAGGAGAGPDSTRVSATPAPPMAPTSAPATSAFG
                     APRHAGPPPVQVGEVLGHTYRIEAFLAKGGMGAVYRARHVVLESEHAIKVILSELAED
                     PQVIALMNQEARTLHTVKNDAVVEYQGLMLDEFGRRYLVMEFVDGPSLASVIKDRRLG
                     PSEVRVLRDRLARGLGAAHEKGVYHRDVSPDNVILPGGNIDNAKIIDFGIAKSTATGE
                     KTLIGNDFAGKYSYASPEQAGMYGGKVDARSDIYSLGLVLASAAIGFGGKLDMGSTLG
                     GMIEARQKLPDLSRVPAELRDELAAMLRPKPEDRVQTMQAVVARGGSGRSATAPAKGA
                     ELPARGGRQGASTSVVIVAAVSFAFVVVAGAAYFLLVPSRGPTTVAHPSSPTTSSPEL
                     AQPQPPALTEPQPPAQPPSQIGLVSPPPPAALSLAERQTQIGEATKGFTCAELKPTVT
                     DAADIRIEGFVSSANDLDRLRSTLIGMPALGPVPLDRVQVYGWPHCEVIKLIDETATP
                     ARPDMAPRLSFNIPSLVYKDGDKLIIQASASPGFDGYLYVDYLDSEGTVLHMLPAPEH
                     SDNHVRAEQSLRLGGDLYAIGAPFGPNLVVAISSQKRLFPPRAEQQESARTYLPVLTK
                     ALEALTSSSTRPVATYTLINTVAR"
     gene            complement(3262..>3501)
                     /locus_tag="VGP50_11135"
     CDS             complement(3262..>3501)
                     /locus_tag="VGP50_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027977.1"
                     /translation="EKLAIDMVQDRVRQDDIFLLCSDGLTKMLEDREIVALLGADRPI
                     EMSVEMLVDKALERGATDNVTVVGVQLRSTAPSDG"
BASE COUNT          567 a         1169 c         1188 g          577 t
ORIGIN      
        1 tcggggcagc ccatcttcgg ttccgacaag ccgacgccgc gggaatcgaa gacgacgaga
       61 tcgcgcgttc tgagccacgg cgcgcgggcg atccactccc accaatggcc gatcgactcg
      121 gcatcgatct gcgccggctc gcccgggccg ccggcgagat agatcacggg atcctcggca
      181 ccgccgggat cgccgcggaa tacgacgaag cgcaaatgga gcaagcgcga cggcgatccc
      241 gtccagcgct ccggcacggc gagactgccg caacgcgcgc tccgcccggc aggcaccgcg
      301 aaccagcaag gcatgtcctc gatgccgggc ttggcgccgg ccggcagcgc ggcagcggca
      361 ttggtcggtg ccaggccccg gtccggggct cggctctgtt cctgtggatc cgcgctgcgc
      421 aaccagacca ggccgaccag cacgatcacg ccggccaatc cgccgccgca agctccccac
      481 agcagggggc cgcgcgccat caacgtccct tcaagcgatc aggcggcgcc ctctccgctc
      541 tgcagaagca gagagggcgc tgcgacacat ttattggccg agattgacca cctgcacccg
      601 ccggttcgcc ggatccgccg ggttcgacgc attgatcggc ttggtctgtc cgcggccgac
      661 ggcttccaag cggccgcgcg gcacgttgta ggcgctgacg agatagctcc tcaccgcctc
      721 cgcgcgccgc ttcgacaagg cgagattgcg gtccggctgg ccggtgctgt cggtatggcc
      781 ttcgaccagg aagtgatact tcgacagctg atccgagttc atggcctcgg cgagttgccg
      841 cacctggtcc ttggcatcgt ccgtcagctc ggcggagccg agcttgaact tgatgtcgag
      901 cccgaccgcg ggcatctccg tcgatccggc gcctgccggc tgcgagttca gcacatggag
      961 tccgcggaag cgcagcggcg gcgtgcctgg cggcggcgtc agctccttga tcagctgatc
     1021 ggaggagacg ttcttgtcgg caggcttctg gtcggatgcg ggcgcgttgt cggccggctt
     1081 cgcgtcctcg gcttgcgcga tcgtcatccc ggcggtgaat gcgaaagcaa tcacggtcgg
     1141 aaaaaggtac cctctcatcg gataagtctc cctgaagtgc gcggttcaca aggtcgcggc
     1201 gcgcatctct accacaaggc acatattttg ccagtaattc tcggcagggg aaagccgttt
     1261 gtcgctcgcg atgtgacaga gatcacaggc ctcatcagac ttccccgggc atagccgcag
     1321 tattaacgcg cgactgtatt gatcagggta taggtcgcga ccggacgcgt actgctcgat
     1381 gtcagcgcct cgagcgcctt ggtcaggact ggcagatagg ttcgggcact ttcctgttgc
     1441 tcggcgcgcg gcgggaaaag ccgcttctgc gaggagatcg ccacgaccag gttggggccg
     1501 aacggcgcgc cgatggcgta gagatcaccg cccaggcgca gcgactgctc ggcccgcacg
     1561 tgattgtcgg aatgctccgg cgccggcagc atgtgcaaga ccgtcccctc gctgtcgagg
     1621 tagtcgacat agaggtagcc gtcaaagccg gggctggcac tcgcctggat gatcagcttg
     1681 tcaccgtcct tgtagacgag gctcgggatg ttgaacgaca gccgcggcgc catgtcggga
     1741 cgcgcgggtg tggcggtctc gtcgatgagc ttgatcactt cgcaatgcgg ccaaccatag
     1801 acctggacgc gatcgagagg cacggggccg agggccggca tgccgatgag cgtgctgcgc
     1861 agccgatcga gatcattcgc gctcgagaca aagccttcga tccggatgtc ggctgcgtcg
     1921 gtcacggtcg gcttgagctc ggcgcaggta aagcccttcg tcgcctcgcc gatctgcgtc
     1981 tgcctctcgg cgaggctcaa agcggcggga ggcggcggcg acacgagacc gatctggctc
     2041 ggcggctgag ccggcggctg cggctccgtc agtgcgggag gctgcggctg ggcaagctcg
     2101 ggagacgatg tcgtcggcga agacgggtgg gcgaccgtcg tcgggccgcg cgagggcacc
     2161 agcaggaaat aggcggcgcc cgccaccacg acgaaggcga atgagacggc agccacaatc
     2221 accacggacg tgctcgcgcc ctggcgcccg ccgcgcgccg gcagttcggc gcctttcgcc
     2281 ggtgcggttg ccgaccgccc gctcccgccg cgcgcgacga cagcctgcat ggtctggacc
     2341 cgatcctccg gcttcggccg cagcatcgcc gcgagctcgt cgcgcagctc ggcaggaacg
     2401 cgcgacaggt cggggagctt ctggcgcgct tcgatcatcc cgcccagggt gctgcccatg
     2461 tcgagcttgc cgccgaagcc gatggccgca ctcgccagta cgaggccaag gctgtagatg
     2521 tcggagcgcg catcgacctt gccgccatac atgccggctt gctcgggcga ggcataggag
     2581 tacttgccgg cgaagtcgtt gccgatcagc gtcttctcgc ccgtggccgt cgatttggcg
     2641 atgccgaaat cgatgatctt ggcattgtcg atgttcccgc ctggaaggat cacattgtcg
     2701 ggcgaaacgt cccggtgata gacgcccttc tcatgcgccg cgcccagacc ccgcgccagg
     2761 cgatcgcgca agacccgaac ctcgctcgga ccgagacgcc ggtccttgat gacgctggcg
     2821 agcgacggac cgtcgacgaa ttccatcacg agatagcgcc ggccgaactc gtcgagcatc
     2881 aatccctgat attcgacgac ggcgtcgttc ttcaccgtat gcagcgtccg cgcttcctga
     2941 ttcatcagcg caatgacctg cgggtcttcc gccagctcgg agaggatgac cttgatggcg
     3001 tgctcgctct cgagcacgac atggcgcgcg cgatagaccg cgcccatgcc gcctttggca
     3061 aggaacgcct cgatccggta ggtgtggccg aggacctcgc ccacctgcac cggcggaggg
     3121 cccgcgtggc gaggggcgcc gaacgcgctg gtggcggggg cgctcgttgg cgccatcggc
     3181 ggcgccggcg tcgcggacac gcgggtggaa tccgggccgg cgcccgcgcc gccggccgtt
     3241 cccggcacgc gcgtgcgatc gtcagccatc gctgggcgcc gtggaccgaa gctggacgcc
     3301 cacgacggtc acattgtcgg tggcgccgcg ttcgagcgct ttgtccacca gcatctcgac
     3361 cgacatctcg atcgggcggt cggcgccgag cagcgcgacg atctcgcggt cctccagcat
     3421 cttggtgagg ccgtcgctgc acagcaggaa gatgtcatcc tggcgcacgc ggtcttgaac
     3481 catgtcgatg gcgagcttct c
//
LOCUS       DASYNS010000357         4871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2897803, whole genome shotgun sequence.
ACCESSION   DASYNS010000357 DASYNS010000000
VERSION     DASYNS010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2897803"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VGP50_11140"
     CDS             complement(<1..806)
                     /locus_tag="VGP50_11140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV8027978.1"
                     /translation="MYRGARYAIVTPYYREDRSLLERCIASVRKQSIAADHILVADGF
                     PQAWLDEAGVRHFKLDRHHGDFGNTPRGVGALIAIGEEYDAIGLLDADNWLEPDHIAL
                     CLAAARVTPAPCDYVVARRTFRRPDETIMPIPEEPGHVDTNCFFFLRGSFGVIPHWTT
                     MPKNLSSFCDRYFYYMLCRQPFTAAAVSKPTVNYHCLWGWMYRALGETTPPDAKPSLD
                     VDKPGRWLRSLNRRELEIASRLVGVSAVPIPAQSAPANDLECPCGSGKRA"
     gene            complement(955..1398)
                     /locus_tag="VGP50_11145"
     CDS             complement(955..1398)
                     /locus_tag="VGP50_11145"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV8027979.1"
                     /translation="MAALDAAPLPAATERSSYALWTSDDIRYADTDRQGHVNNAVYAT
                     FCETARVGFLYDGALDLHGPSASFAIVRLELDFRAELFYPATVDIGTRVLAVGQRSFR
                     LGHGIFSGALCAATAECVMVLIDNATRKATALTPQARTWLEARRP"
     gene            complement(1398..2612)
                     /locus_tag="VGP50_11150"
     CDS             complement(1398..2612)
                     /locus_tag="VGP50_11150"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV8027980.1"
                     /translation="MTDTIPAGALAGLKVIDLSRVLGGPFCTQMLGDHGADVIKVEPP
                     QGDETRGWGPPFLDGAASYYIGVNRNKRGIALDLTKPEGCAVLFRLLEGADVLVENFK
                     TGTLERWGLGYDAVLKQRFPRLIHCRISGFGADGPLGGYPGYDAAVQGMSGLMSLNGE
                     AEGEPLRVGIPVVDIATGLNAAIAILMALQERTRSGSGQFVEATLYDCALALLHPYAA
                     NWFLSKKPPKRTGNQHPNVAPYDQFRTKTKRIFLAIGNNRQFQRFCAEIGRSALSEDA
                     RFRDNKDRTQNRVALRAELEAALADVDGDALTMRLLDRGVPCGVVQELPDALTHPHTL
                     HRGMIYDDGAYRGVGNPIKMSRTPPALRRPPPHFGEASRAVLKEAGYGDAEIERLIQS
                     GVVATMPQKDEE"
     gene            complement(2734..3162)
                     /locus_tag="VGP50_11155"
     CDS             complement(2734..3162)
                     /locus_tag="VGP50_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV8027981.1"
                     /translation="MKRHSKIGLLVAVGAAVVLSAAGVAATVRTVQEAALSQSKAGQG
                     RVLFAQVCRSCHTGEPDKHKAGPSLYGVVGRPAGSASGFDYSDAMKLASVTWTDESLD
                     RYLADPKHFVPGNRMPYSLLMGAENGKRREQVIAYLHTLQ"
     gene            3506..4117
                     /locus_tag="VGP50_11160"
     CDS             3506..4117
                     /locus_tag="VGP50_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEV8027982.1"
                     /translation="MSIDFLATSLIVVVSPGTGVLYTLAAGLARGSRASVVAAFGCTL
                     GIVPHMAAAILGLAAILHTSALAFQSFKYLGVAYLLYMAWNALRERGALRVETQVGAR
                     SALQVTVNAVLINILNPKLSIFFFAFLPQFVGADEAHPLSRMLVLSGVFMLMTFVVFV
                     AYGLFAAAIRNHVIARPRVLTWMRRAFAGAFVALGAKLALADR"
     gene            complement(4200..>4871)
                     /locus_tag="VGP50_11165"
     CDS             complement(4200..>4871)
                     /locus_tag="VGP50_11165"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEV8027983.1"
                     /translation="QLLSPDGALVSLQQSPEHNVFTDGGRELFDSAGQGRIDVFFLSG
                     AQIDGAGNINLTVIGDYARPSRRFGGAYGSAYLYFMVPRVILFRLEHSRRSLVERVDF
                     ITAPGTSPPHVFRRGGPAALVTPLSVFAFDKAKARFRLESIHRGHSLEEVLDQTGFVF
                     ERPEIVPETPLPSPATLSLLRERVAPQLAQIYPRFAQAVFGLPLGCSSSERGGRRGCA
                     VDAEG"
BASE COUNT          795 a         1551 c         1707 g          818 t
ORIGIN      
        1 tcggccctct tgccggagcc gcaggggcat tcgaggtcat tcgccggcgc ggactgggcg
       61 gggatcggta ccgccgatac gccgaccagg cgactggcga tctccagctc gcggcggttc
      121 agcgagcgca gccagcgccc cggtttgtcg acatcgagcg acggcttcgc gtccggcggc
      181 gtcgtttcgc cgagcgctcg atacatccat ccccagaggc aatgataatt caccgtgggc
      241 ttcgacaccg ccgccgcggt gaagggctgg cggcacagca tgtaatagaa atagcggtcg
      301 cagaaggacg agagattctt cggcatcgtc gtccagtgcg ggatgacgcc gaaggagccg
      361 cgcaggaaga agaagcagtt ggtgtcgacg tgaccgggct cctcgggaat cggcatgatg
      421 gtctcgtcgg ggcggcgaaa ggtccgtcgc gcaaccacat agtcgcaggg cgcgggcgtg
      481 actctcgccg cggcgaggca gagggcgatg tggtcgggct cgagccagtt gtcggcgtcg
      541 agcaggccga tggcgtcgta ttcctcgccg atcgcgatca gcgcgccgac gccgcgcggc
      601 gtgttgccga agtcgccgtg gtggcgatcg agcttgaaat ggcgcacgcc cgcttcgtcg
      661 agccaggctt gcggaaagcc gtcggcgacg agaatgtgat ccgcagcgat cgattgcttg
      721 cgaacgctgg cgatgcaacg ttcgagcagc gaccgatctt ctcgatagta gggtgtgacg
      781 attgcgtaac gcgcgccgcg atacatagag tctcttttgt ttgtgcccgc catgatagcg
      841 cggccatggg cggcaccgag atcacgttct tgcgaacttc ggcggccgct ttcttgagca
      901 agacaccgag actttcgagc aagtgtttcc gtctttagag ggagtgcacg gcactcacgg
      961 gcggcgtgcc tcgagccatg tgcgggcttg cggcgtcagc gcggtcgcct tgcgcgtcgc
     1021 gttgtcgatc aggaccatca cgcattccgc cgtagcggcg cagagcgctc cgctgaagat
     1081 gccgtgtccc aggcgaaacg agcgctggcc gaccgcgagc acgcgcgtgc cgatgtcgac
     1141 ggtcgcggga tagaagagct cggcgcggaa gtcgagctcg aggcggacga tggcgaagct
     1201 ggcggagggg ccgtgcaggt cgagcgcgcc gtcgtaaagg aagccgaccc gcgcggtctc
     1261 gcagaacgtc gcgtagacgg cgttgttgac atggccctgg cggtcggtgt cggcatagcg
     1321 gatgtcgtcc gacgtccaca gcgcatagga cgagcgctcg gtcgccgccg gcagcggcgc
     1381 cgcatcgagg gccgccatta ctcctcgtcc ttctgcggca tcgtcgcgac gacgcccgat
     1441 tggatcaggc gttcgatctc ggcatcgccg tacccggcct ccttcagcac ggcgcggctc
     1501 gcctcgccga aatgcggtgg cggccggcgc agcgccggcg gcgtgcgcga catcttgatc
     1561 gggttgccga cgccgcggta agcgccgtcg tcatagatca tgccgcgatg aagcgtgtgc
     1621 ggatgggtca gcgcgtccgg cagctcctgc acgacgccgc agggcacgcc ccggtccagg
     1681 aggcgcatgg tcaaggcatc gccgtcgacg tcggcgagcg ccgcctcgag ctcggcgcgg
     1741 agtgcgacgc gattctgcgt ccggtccttg ttgtcgcgaa agcgtgcgtc ctcgctgagc
     1801 gcgctccggc cgatctcggc gcagaagcgc tggaattgcc ggttgttgcc aatcgccagg
     1861 aagatgcgct tggtcttggt gcggaactgg tcgtagggcg cgacattggg atgctggttg
     1921 ccggtgcgct tcggcggctt cttcgacagg aaccagttgg cggcgtaggg gtggaggagg
     1981 gccagcgcgc aatcgtagag cgtcgcttcg acgaactggc cgctccccga tcgcgtgcgc
     2041 tcttgcagcg ccatcaggat ggcgatagcg gcgttgaggc cggtcgcgat gtcgaccacg
     2101 ggaatgccga cgcgcagcgg ctcgccctcg gcctcgccat tgaggctcat gagccctgac
     2161 atgccctgca cggcggcgtc gtagccggga tacccgccga gcggcccgtc cgcgccgaag
     2221 cccgagatgc ggcagtggat gaggcgcgga aagcgctgct tcagcacggc gtcatagccc
     2281 aggccccacc gctcgagcgt gccggtcttg aagttctcca ccagcacgtc ggcgccctcg
     2341 agcagccgaa acagcacggc gcatccttcg ggcttggtga ggtcgagcgc gatcccgcgc
     2401 ttgttgcggt tgacgccgat gtaataggag gcggcgccgt cgaggaaggg cggcccccaa
     2461 ccgcgcgtct catcgccttg cggcggctcg accttgatga cgtccgcgcc gtggtcgccc
     2521 agcatctgcg tgcagaaggg accgcccagc acgcgggaga gatcgatcac tttgagcccg
     2581 gcgagcgcgc cagcgggtat ggtgtcggtc atggcccttg tccctacagc caagtgaagc
     2641 tgggacgcaa gtcctgaccg gacgcttgtt cattgccgca tccaccgcgg gccggctttc
     2701 gcacccgtac gagtgagcgt cgtcacgcct gattcactgg agcgtatgca gataggcgat
     2761 gacctgctcg cggcgtttgc cgttctcggc gcccatcagt agcgaatagg gcatgcggtt
     2821 gccgggcacg aagtgcttgg gatccgcgag atagcggtcg agcgactcgt cggtccaggt
     2881 cacgctcgcg agcttcatcg cgtccgagta gtcgaagccg gacgccgatc ctgcaggacg
     2941 cccgaccaca ccatagagcg aggggcccgc cttgtgcttg tccggctcgc cggtatggca
     3001 ggaccggcag acctgcgcga agagcacgcg gccctgcccg gccttgcttt gcgacagcgc
     3061 cgcctcttgt accgtgcgca ccgtggccgc tacgccggca gccgagagaa caacggcagc
     3121 accgaccgcc acgagcaggc caatttttga gtgtcgcttc attcttggaa actccctttc
     3181 gaaccacgcc cgcttcgcat atcagcgcac cgggtcgctt gattcgacaa tgacagcgaa
     3241 ccggcgagtg atggtgtcac gtcacataac aactaagtga cggcgaggtg atttgggcgc
     3301 aacgacaaaa ccgagcttcg tcgaccgagc gtgcgcggcg gcaaggaaag atgaatgccg
     3361 aggacgcgga gggcaccgcc gagggcgcgg aggagtggtg gtccattgcg ccctccgcgt
     3421 tcgctaacgc tatagtgtgg agcagaggcg agggcggcgc ttggctctga agtcgccatc
     3481 gccatcgcaa tcgcgtgagg gcgccgtgag tatcgacttc ctggcaactt cgctcatcgt
     3541 cgtcgtgtcg cccggaacgg gcgtgctcta tacgctggcg gcggggctcg cccgcggctc
     3601 gcgtgccagc gtcgtcgcgg ctttcggctg cacgctcggc atcgtgccgc acatggcggc
     3661 cgccatcctg gggctggcgg cgatccttca caccagcgcg ctcgcgttcc agagcttcaa
     3721 atatctgggc gtcgcgtatc tgctctacat ggcatggaac gcgctccgcg agcggggagc
     3781 gctccgcgtc gaaacgcagg tcggcgcccg gtcggcgctt caggtgaccg tcaacgccgt
     3841 cctgatcaac attctcaatc cgaagctgtc gatcttcttc ttcgccttcc tgccgcaatt
     3901 cgtcggcgcc gacgaagcgc acccgctgtc acgcatgctg gtgctgagcg gcgtcttcat
     3961 gctgatgacc ttcgtcgtct tcgtcgccta cggcctcttt gccgcggcga tccgcaacca
     4021 cgtcatcgcc cgcccgcggg tccttacatg gatgcgtcgc gctttcgccg gcgccttcgt
     4081 cgcgctcggc gccaagctcg cgctggccga ccgctagccg cacttagaag cggagcatgg
     4141 cgaaaaagga agatgaacgc ggaggccgcg gaggttgcgc ggtggacgcg gaggagcggt
     4201 cacccctctg cgtccaccgc gcaacctctg cgccctccgc gttcgctgga cgagcagccc
     4261 aggggaaggc cgaaaacggc ttgcgcgaag cgaggataga tctgcgccag ctgcggcgcc
     4321 acgcgctcgc gcagcagcga cagcgtcgcg ggtgagggga gaggcgtctc gggcacgatc
     4381 tccggtcgct cgaagacgaa gcccgtctgg tcgagcactt cctcgaggct gtggccgcga
     4441 tggatgctct cgaggcggaa gcgcgctttg gccttgtcga aggcgaagac cgagagcggc
     4501 gtcaccagcg ccgccgggcc gccgcggcgg aagacatggg gcgggctggt gcccggcgcg
     4561 gtgatgaagt cgacgcgctc gacgaggctg cgccgcgaat gctcgagccg gaacaggatc
     4621 acgcgcggca ccatgaaata gagataggcc gagccatagg cgccgccgaa gcgccggctg
     4681 ggccgcgcgt agtcgccgat gacggtgagg ttgatgttgc cggcgccgtc gatctgcgcg
     4741 cccgagagga agaagacatc gatgcgcccc tggccggcgc tgtcgaagag ctcgcggccg
     4801 ccatcggtga agacgttgtg ctccggactc tgctgcagcg ataccagggc gccgtcgggc
     4861 gagagcagct g
//
LOCUS       DASYNS010000358         4006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2273184, whole genome shotgun sequence.
ACCESSION   DASYNS010000358 DASYNS010000000
VERSION     DASYNS010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2273184"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VGP50_11170"
     CDS             complement(<1..196)
                     /locus_tag="VGP50_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027984.1"
                     /translation="MDGFAARATLDALIAEVETELSVGREIERFLDGESDGHVLLNAL
                     YGDVLDEPVPERLLATLRENC"
     gene            483..2177
                     /locus_tag="VGP50_11175"
     CDS             483..2177
                     /locus_tag="VGP50_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV8027985.1"
                     /translation="MNFLNLLRIKAKLAIIVAICALALPVALAFAASLQRERMMDDRL
                     AELRALVDVAYGLADSLEKEAVAGTITREDAIARFRSDLRAMWYDNHENYLVMGSMDN
                     RWMLHPAVPQVEGTEGSKDIDKPGTYLITYFTEALASADDAIIHYRYARPGQEEKLAK
                     TTFARKFKPWNAFIASGVYVGDIDDAYRAVLLRLSLLSAIVLAIAALMAYGVSRNISG
                     PLASLKAKMEKLAAGDLAFDASETQRRDEIGEMARTVKVFKDNALAMQRLRIDQEELK
                     KSAERERKQTLELMAGNFERQVRGVVAAVSSAATQMQSSARTMSASADQTREQTLAVA
                     AGAEQATGNVHTVAVASQELSASISDIGRQVSAAATVARQAADEGQRTNAAISGLSDA
                     AHKIGEVVALINDIASQTNLLALNATIEAARAGDAGKGFAVVASEVKALATQTARATD
                     DIRAQIGAIQSETGSAVTAIQSISKTILTVNQISSAIAAAVEEQAAATHEITRNVQQA
                     ASSTQDVSHNIAGVNSAVQNAGAIADAVLGAANELARQAQLLSREVDGFLASVRAA"
     gene            2264..2677
                     /locus_tag="VGP50_11180"
     CDS             2264..2677
                     /locus_tag="VGP50_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV8027986.1"
                     /translation="MTEQDAQAFVDRFAAAWAGRDGAAFLALWHPDGQLHYPFASRIL
                     KGSEIGKLNDLQVAVAPHLTWTLLGWTSRGNVVVIEWESSNRYGERTLRWRGVDKMTL
                     VDGKIAEEIVYADTAPLQAVRQGRNFDALIQIPWP"
     gene            complement(2900..3859)
                     /locus_tag="VGP50_11185"
     CDS             complement(2900..3859)
                     /locus_tag="VGP50_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019085477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEV8027987.1"
                     /translation="MGLHSLLRMKPDIVLTIPIYASAVAQLDDAFTVHRLWQAKHHAA
                     FFAEIGDRARAIVTGAYPVTAELIDALPKTEIIATQSVGLDHIDLKAAKARGIAVTNT
                     PDVLTDDVADLGMALLLAVARRIVVGDRHVRNGLWLKNGLPLASKVGGTTLGILGLGR
                     IGLAIARRAEAFGMTVVYHGPRPKADIPYRYDADLAAMARASDYLMVSCPGGAATRHL
                     VTAEVLAALGPAGVVINVARGSVIDEEAMVKLLVEGKLGGAGLDVFADEPRVPEALFA
                     LDNVVLQPHVGSATHETRGAMGQLVVDNLKAHFAGRPLLTPVG"
BASE COUNT          717 a         1346 c         1318 g          625 t
ORIGIN      
        1 ggcagttctc gcgtagcgtc gccagcagcc gctcgggaac gggctcgtcg agcacgtcgc
       61 cgtaaagggc gttgaggagg acgtggccgt cgctctcgcc gtcgaggaag cgctcgatct
      121 cccgcccgac ggaaagctcc gtttccactt cggcaatgag agcgtcgagc gtcgcgcgcg
      181 cagcaaatcc gtccatgtgc ccgatatcct tcgatctatg ccaacccctt gggggcctag
      241 gcggtggttt gccttacaaa cggttacggt ctgttacggt tccaacccgg accgacattt
      301 ttgtccccaa agcgcgggat cgggcgcttt cggttttctc ggcgaagagc cccggaccac
      361 ccgggcgacg gccgacgagg ctaggcgatt gaccgtccgt ttaccacgcc tttagcaatt
      421 gctgcttcaa ttcaccccga tgggtgccgg ccggaggccg cggtgggggg tgaggcaacg
      481 ccatgaattt tctcaatctt ctcaggatca aggcgaagct cgccatcatc gtcgccatct
      541 gcgcgctggc gctccccgtc gccttggcct tcgccgcatc gctgcagcgc gagcgcatga
      601 tggacgaccg gctggccgag ctccgcgccc tggtcgacgt cgcctatggt ctcgccgact
      661 ccctcgagaa ggaagcggtg gctggaacca tcacacgcga ggacgcgatc gcgcgcttcc
      721 gcagcgatct ccgcgccatg tggtacgaca accatgagaa ctacctcgtc atgggctcga
      781 tggacaatcg ctggatgctt catccggcgg tcccccaagt cgaaggcacc gagggcagca
      841 aggacatcga caagcccggc acgtatctca tcacctattt cacggaggcg ctcgcctccg
      901 cggatgacgc catcatccac taccgctatg ccaggccggg acaggaagaa aaactggcga
      961 agaccacgtt tgccaggaaa ttcaagccgt ggaacgcatt catcgccagc ggcgtctatg
     1021 tcggcgatat cgatgatgcc taccgcgcgg tgctgctgcg gctcagcctg ctcagcgcga
     1081 tcgtcctcgc catcgcggcg ctgatggcct atggcgtcag ccgcaacatc agcggtccgc
     1141 tcgcgagcct caaggccaag atggagaagc tcgcggccgg cgatctcgcc ttcgacgcca
     1201 gcgagacgca gcggcgcgac gagatcggcg agatggcgcg gacggtcaag gtattcaagg
     1261 acaatgcgct cgccatgcag cgcctccgca tcgaccagga ggagctcaag aagagcgccg
     1321 agcgcgagcg caagcagacg ctggaattga tggccggcaa tttcgagcgc caggtgcgcg
     1381 gcgtcgtcgc cgccgtctcc tcggcggcga cgcagatgca gtcgagcgcg cgcaccatgt
     1441 cggcgagcgc cgatcagacg cgcgagcaga ccctcgccgt cgccgccggc gccgagcagg
     1501 ccaccggcaa cgtccacacc gtcgccgtcg cctcgcagga gctctcggcc tcgatcagcg
     1561 acatcggccg ccaggtgagc gcggccgcga ccgtcgcgcg ccaggccgcc gacgagggcc
     1621 agcgcaccaa cgcggcgatc tccggcctct cggacgctgc gcacaagatc ggcgaagtgg
     1681 tggcgctcat caacgacatc gccagccaga ccaacctcct cgccttgaac gcgacgatcg
     1741 aggccgcgcg cgccggcgat gccggcaagg gcttcgccgt ggtggcgagc gaggtcaagg
     1801 cgcttgccac tcagacggcg cgggccaccg acgacatccg cgcgcagatc ggcgccattc
     1861 agagcgagac gggatcggcc gtgacggcga tccagagcat ctccaagacc attctcacgg
     1921 tgaaccagat ctcctcggcg atcgccgccg cggtcgagga gcaggccgcc gccacccatg
     1981 agatcacgcg caacgtgcag caggccgctt ccagcacgca ggacgtctcg cacaacatcg
     2041 cgggcgtgaa cagcgccgtg cagaacgccg gcgcgatcgc cgacgccgtg ctgggcgccg
     2101 ccaacgagct cgcccgccag gcccagctcc tgagccgcga agtcgacggc ttcctcgcga
     2161 gcgtccgggc ggcgtaacag tcgtcacgat gaccgcgtcc gatctccgcc agtaaccccg
     2221 gccgcggctc gttataagga agcatcaact tcggggccga gacatgaccg agcaagacgc
     2281 ccaagcgttc gtcgaccggt ttgccgcggc ctgggccggc cgcgacggcg ctgccttcct
     2341 cgccctttgg catcccgacg gccagcttca ctatcccttc gcgagccgca tcttgaaagg
     2401 cagcgagatc gggaagctca acgatcttca ggtcgccgta gcgccgcatc tgacttggac
     2461 gctgctcggt tggacgtcgc gtggaaatgt ggtcgtgatc gagtgggaga gctccaatcg
     2521 ctatggcgag cggacgctgc gctggcgcgg cgtcgacaag atgacattgg tcgacggcaa
     2581 gatcgccgag gagatcgtct acgccgacac cgcgccgctg caagccgtgc gccagggccg
     2641 caactttgac gccttgatcc agatcccgtg gccttaagca gacaacgaca gtcccctttc
     2701 tcgggcttcg gcacatcgca ccgcgttctg tttgcaaaat tattgggaga aatgcggcgc
     2761 cgaagattgt gcgccgctga tccgcgacgg tcatcgcgtc accgtcgatc tggcggggcg
     2821 acaggaatag gagggaccct atcccgtccc gccgcgggag aaggccgaca accgataact
     2881 gacaactaag accttacaac tacccgaccg gcgtcagcag cggccgcccc gcgaaatgcg
     2941 ccttcagatt gtcgacgacg agctggccca tggcgccgcg cgtctcatgg gtcgcgctgc
     3001 ccacatgcgg ctgcagcacg acattgtcga gagcgaagag cgcctcgggc acgcgcggct
     3061 cgtcggcgaa gacgtcgagg ccggcaccgc ccagcttgcc ctcgaccagc agcttcacca
     3121 tggcctcctc gtcgatcacc gagccgcgcg ccacgttgat gacgacgccc gcggggccga
     3181 gcgccgccag cacctcggcc gtgacgaggt gacgcgtggc ggcgccgccg gggcagctca
     3241 ccatgaggta atcgctcgcg cgcgccatgg cggcgagatc cgcgtcgtag cgatagggta
     3301 tgtccgcctt gggacgcggc ccgtgataga cgaccgtcat gccgaaggcc tcggcgcggc
     3361 gcgcgatggc gaggccgatg cggccgaggc cgagaatgcc gagcgtcgtg cccccgactt
     3421 tgctggcgag cggcagcccg ttcttgagcc agagcccatt gcgcacatgg cggtcgccga
     3481 cgacgatgcg ccgcgccacc gcgagcagca gcgccatgcc gagatcggcc acgtcgtcgg
     3541 tgagcacatc gggcgtgttg gtgacggcga tgccgcgcgc cttcgcggcc ttgaggtcga
     3601 tgtgatcgag gccgacgctt tgcgtggcga tgatctcggt cttgggcagc gcgtcgatca
     3661 gctccgcggt taccggataa gcaccggtga cgatggcgcg cgcgcggtcg ccgatctctg
     3721 cgaagaaggc cgcgtgatgc ttcgcctgcc atagccgatg gacggtgaag gcgtcgtcga
     3781 gctgcgccac ggccgaggcg tagatcggga tcgtcaggac gatgtcgggc ttcatgcgca
     3841 agagagaatg cagacccaac ggcgaacgcg caagcggtcg cctgtggcgc ccggactatc
     3901 tgtcgttccg tcctgcctct tgcgctgcgg cggtaattgc cattaagtgt catatttaag
     3961 aaggacggga ggggggcatg gcggtaccga gaggtgccgg ccgacg
//
LOCUS       DASYNS010000359        14975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2898223, whole genome shotgun sequence.
ACCESSION   DASYNS010000359 DASYNS010000000
VERSION     DASYNS010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 14975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14975
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2898223"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VGP50_11190"
     CDS             <1..1049
                     /locus_tag="VGP50_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEV8027988.1"
                     /translation="DPRLQAAAEAAVEATLSRQGEKAGVEQAALVAMSPDGAVRAMVG
                     GRNYGESQFNRVTQALRQPGSAFKPFVYLAAFEHGLGPNDRFVDQPIRIGNWQPHNYE
                     GTYRGEITVTDALAHSVNTVAAQVLQRVGVDNVIATAHRLGITSELARDPSLALGTSE
                     VSLIDLTSAYCAFASGGVGALPYGIFEIRDRDGTVLYKRHGDGGARVISPELAGMMND
                     MLAEVLSRGTGRAAALDRPAAGKTGTTQDFRDAWFVGYTADLVAGVWLGNDDDTPTKH
                     VIGGTLPARTWRAFMVAASEGTAARPLPSAPQVALTGSSSGGWLSRLLHNIFGSDSRA
                     TPQHRDPSAPRPNG"
     gene            1152..3836
                     /locus_tag="VGP50_11195"
     CDS             1152..3836
                     /locus_tag="VGP50_11195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEV8027989.1"
                     /translation="MSVRNLDKLMKPKSLALIGATPRTGAVGAVVLRNLRRAGFQGPL
                     MLVNPKHHALDGMPVYRDVQSLPETPDLAVIVTPPEAVPGLIATLGARGTRAAVVITA
                     GFGELGERGRALQQATLEAAQPHLLRLVGPNCIGVMVPGAGLDASFSHIAPKAGDLAF
                     VSQSGAMITAVLDWAAPRGIGFSHVVSLGDMADVDFGDMLDYLAEDGDTRAILLYVEA
                     ITHARKFMSAARAAARAKPVLAVKVGRFAESARAAASHTGVLAGSDAVYDAAFRRAGM
                     LRVDTMAELFDAVETLALTRPQQGERLAIVTNGGGPGVLATDALIAAGGRLAELSPDI
                     VAKLDAVLPRSWSHANPVDIIGDAPGKRYADTLRILMAEPAIDAVLALNAPTAMARPG
                     EAAQAVIEEFKATPQSRLVGRNLLTAWLGEHSAAPARRRFAEARIASYETPDGAVRGF
                     MHRVQYRRNQDLLMETPAARPDDFTPDATTAHQAIARTVAAGGGWLAAEDAAAVLGAY
                     GIPLPASLVVADAAGAADAASAIGFPVALKIRSPDLTHKSDVGGVVLNLGTAEGVRTE
                     ATLMLERVKAAQPRARIDGFLVQAMVQRPGALELILGLIDDPTFGPVVMFGQGGTAVE
                     LLHDTTLELPPLNAALARAQMARTGVWRLLQGYRGQPPAAIDAIADTLIRVAQLAADH
                     PEIRELDINPLLADQKGVLALDARIGVAPAHIPGAARLAISPYPKEFESTATLRDGTV
                     IRLRPVRPEDEPLLQDLARHMNADDLRLRFFTPVTTLSHQLLARLSQIDYDRAMALIA
                     RPAVEGTALGIVRFAADPDNRRGEFAVSVRSDWKGRGLGYLLMTRIIEVARQRDLGEI
                     FGEVLRENGAMLRVARALGFSVTTHPQEAHLLRLTKPL"
     gene            complement(3859..4716)
                     /locus_tag="VGP50_11200"
     CDS             complement(3859..4716)
                     /locus_tag="VGP50_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027990.1"
                     /translation="MAALEGESGRVKVPIDRVAAAAYRVVFGRPGRVLEVAWLPMLVL
                     LAVELLPRLISDYFWPDDTAAAASLPGADIAETVIVMLCLSAFAVRWHHLVLAADRPL
                     PLRRFLAVWARFLGYSLPFYLVMLAVAAGAPVALHALSAAITDHPIAGLSELFTENTA
                     DALVAVIALAISLVVARCSLVFPAVVIGQPLGLAGAWRHMRGNTWRFLAATLLVTTPI
                     LLLVWLLLDLILEGAHLPLPDPLAPPPTELFLLNGVLTIVLLFIFTALGATVLADFYR
                     RLVLHVRSN"
     gene            complement(4795..5487)
                     /gene="phoB"
                     /locus_tag="VGP50_11205"
     CDS             complement(4795..5487)
                     /gene="phoB"
                     /locus_tag="VGP50_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEV8027991.1"
                     /translation="MRPLVLVVEDEPALVTLLRYNLEREGFAVNEAHDGEEALLQLRE
                     SKPDAVLLDWMLPRISGLEVCRQIRRAPAWRDLPVIMLTARGEEGDRVRGLDSGADDY
                     VVKPFSPGELIARLRAVIRRVRPAASAETLRFGDIAMDLSAHRVTRGDSAVHLGPTEF
                     RLLRFLLERPGRVFSREQLLDGVWGRDAEVELRTVDVHIRRLRKALNNGSRPDVIRTV
                     RAAGYALDLVAA"
     gene            complement(5487..6236)
                     /gene="phoU"
                     /locus_tag="VGP50_11210"
     CDS             complement(5487..6236)
                     /gene="phoU"
                     /locus_tag="VGP50_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV8027992.1"
                     /translation="MVSEHIIKSYDEELQRLDNIITQMGGLAESQLAAAIDAVVKRDS
                     DFASEVIENDARIDALERDTESLVMRLLALRQPMARDLRQSIAALKIASDLERVGDYA
                     ANVAKRAIALNQTPPVRPVYAIPRMGRLGQAMIKDILDAYVERDADKALAVWLRDEEL
                     DEMYTSLFRELLTYMIEDPRNITACTHLLFIAKNLERIGDHTTNIAETLYFLVHGTPL
                     AQTRPKGDRSSLAVVRGAPPPIEHDDHQEDE"
     gene            complement(6248..7102)
                     /gene="pstB"
                     /locus_tag="VGP50_11215"
     CDS             complement(6248..7102)
                     /gene="pstB"
                     /locus_tag="VGP50_11215"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEV8027993.1"
                     /translation="MTADFALTASEAARPPTLGRPIVGPRGPVAAAQTAPKVVIKDVR
                     FYYKQFEALKGINLVLHDKRVTAFIGPSGCGKSTLLRILNRIYQLYPGQRATGEVLVD
                     GRNILEPGQDLNLLRAKIGMVFQKPTPFPMSIYDNVAFGIRLYERLTPGALDERVEEA
                     LRRAALWEEVKDKLRQSGLSLSGGQQQRLCIARAVAVKPEILLLDEPASALDPISTQR
                     IEELIAELKSDYCIAIVTHNMQQAARSSDYTAFMYLGELVEFSETEALFTHPREKRTE
                     DYITGRFG"
     gene            complement(7099..7953)
                     /gene="pstA"
                     /locus_tag="VGP50_11220"
     CDS             complement(7099..7953)
                     /gene="pstA"
                     /locus_tag="VGP50_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV8027994.1"
                     /translation="MQSGGLYARRRAVNAVYLTLSWTATGIGLAVLFLILGELLWRGI
                     PALSVDFFTKETPGPGSTGGGMANAIIGSVFMTGLATLVGTPVGILTGTYLAEFGRGS
                     RIAPAVRFVNDILLSAPSIIIGVFVYGLIVVPMHGFSAWAGSAALAIIVVPVVVRTTE
                     NMLGLVPGSLREAAVALGAPQWKVIVMVAYRAALQGMLTGVMLAVARIAGETAPLLFT
                     VVSNQFTSLDMNREMASLPVVIFQFANSPYDNWVDLAWGGAMLITLSILLLNIVARLL
                     ASWSNRTP"
     gene            complement(7953..8918)
                     /gene="pstC"
                     /locus_tag="VGP50_11225"
     CDS             complement(7953..8918)
                     /gene="pstC"
                     /locus_tag="VGP50_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV8027995.1"
                     /translation="MASALTAATRRRLDTQHLQDAAFRSATQLFAVGVLVLLGAILLA
                     LAVGAAPAFEKFGWRFLINQSWNPVTENFGALPAIYGTLVTSLIAMVIAVPLGFGIAV
                     FLTELCPPPFRRPIGTLIELLAGIPSIIYGIWGFFVMRPIMAAYVQPALTDSLGQVPV
                     IGALFSGPPFGLGVLTAGIILAFMVLPFVAAVTRDVFLTVPPMLRESAYGVGATTTEV
                     VRDIVLPYTRTGVIGGIMLALGRALGETMAVTFVIGNANRLQPSLLSPGTTISATLAN
                     QFAEAKDDMYVSALFALGLILFVITFLVLAAAKLMLLELEQRAAK"
     gene            complement(8903..9964)
                     /gene="pstS"
                     /locus_tag="VGP50_11230"
     CDS             complement(8903..9964)
                     /gene="pstS"
                     /locus_tag="VGP50_11230"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV8027996.1"
                     /translation="MQIRSKTTAWLGAIALLGAAVLSAPASAIEISGAGATFPYPIYA
                     KWAEAYKAKTGVSLNYQSIGSGGGIKQINAKTVDFGASDMPLKPEELEKADLTQWPMV
                     MGGVVLVVNLPNVKPGDLTLDGPTIADIYLGKIAKWDDPAIKKLNPGAKLPATAIAPL
                     YRSDGSGTNFIFTNYLSKVSPEFKSKVGENTSVEWPTGVGGKGNEGISALAATTPGAI
                     GYVEYAFALQKKLAYTKLINKEGSTVAPDSKSFQTAAANADWRNAPGFYLLLTDQSGK
                     ESWPITGATFILIHKVQDKPEVAKQVLDFFAWSYQNGSTMAEELDYVPLPEGVIKMVE
                     ASWAANIKDSSGKPVWQAH"
     gene            complement(10110..11501)
                     /locus_tag="VGP50_11235"
     CDS             complement(10110..11501)
                     /locus_tag="VGP50_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8027997.1"
                     /translation="MSATARWLLGAVILSSPAFVVLGVIAAGGFIGASGAALAALAVY
                     AAVLVLLRPLVMGIAKVQAAVDALAAGDGAAPDVATLSPAVRELWLAVGRWARASRQK
                     LAASAVELGAAQAVLAALPDPLILLDDRRRILRANAAANELLGMRLVERDLAVAIRHP
                     AVLDAADAVLRGGASRVVDFDVTTPVDRHLSARLAPLRPHTPEGAAAVLTLHDLTAAK
                     RSERLRADFAANASHELRTPLASLTGFIETLRGPARDDGAARSRFLAIMAEQAQRMAR
                     LVDDLLSLSRIEMNEHRLPTDRVHIGRVLHAVGDLLEQRAEARNMQLILELPEGLPEV
                     QGDPDELAQVFQNLLDNAIKYSRPGTPIEVTARPSSRFLPGARAGEKPAALAIAVRDH
                     GDGIAREHLPRLTERFYRVDPARSRELGGTGLGLAIVKHIVNRHRGALEIDSEIGEGS
                     TFTVYLPLPMSAP"
     gene            complement(11643..13913)
                     /locus_tag="VGP50_11240"
     CDS             complement(11643..13913)
                     /locus_tag="VGP50_11240"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEV8027998.1"
                     /translation="MDKPDARVTEEEALQFHAEGRPGKLEVTPTKALTTQHDLSLAYS
                     PGVAFPCLHIARDASLAYDYTAKGNLVAVISNGTAVLGLGDLGALAAKPVMEGKAVLF
                     KRFAGIDGVDLEIDTKDVDAFVNCVRYLHPSFGGINLEDIKAPECFIIEQRLRELLDI
                     PVFHDDQHGTAIIATAALLNALDLTGRDLKTIKMVVNGAGAASIACVELLKSMGLAHD
                     NVILCDTKGVVYQGRSEGMNQWKSAHAINTKRRTLTEALDGADVFFGLSVKGAVSQAM
                     VKGMAPRPMIAAMANPDPEITPEDVRAVRPDAIICTGRSDYPNQINNVLGFPYIFRGA
                     LDVRASTINEAMKIAAAHALAKLAREDVPDEVAAAYSGQRLQYGPDYIVPTPFDPRLI
                     WAVPSAVAKAAMDSGVARRPIANLDAYRATLRGRLDPTAATLQRTFDAVRASPKRVVF
                     AEGEEEKTIRAALSFRDSGYGTPVLIGREERIRERLRQMGLAAAGELEIHNARLSHAN
                     KTYTQFLYGRLQREGALYRDCQRMVNQDRNTFAACMVACGDADALVTGATRSYFTAFE
                     EVTRVIRAKPGHRICGYALLLSRGRSVFIADTTEHALPDAEELADIAVQSAHRARQMM
                     GQQPRVALLSYSTFGNPPGEAGRRAHEAVELLDARQVDFEYDGEMSAEVALNLALMRE
                     RYPFCRLTGPANVLIMPGLNSANIASQLLEQLGGGTRVGPLLMGLERPAQIVEMGATV
                     SDLVNLAALAAHEAIE"
     gene            complement(14092..14415)
                     /locus_tag="VGP50_11245"
     CDS             complement(14092..14415)
                     /locus_tag="VGP50_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8027999.1"
                     /translation="MHAFDTVDAFLLATYRLRLEDVLCEGCAMGRLSVVLAERATIVP
                     RLVQSLQEEDGGIANDYEGILVLDGVWYRFRCHLFIDPSGQRFLTDVTGFEAVEWRAR
                     MAMPA"
     gene            14698..>14975
                     /locus_tag="VGP50_11250"
     CDS             14698..>14975
                     /locus_tag="VGP50_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028000.1"
                     /translation="MKTRKTATPALRRMLHGSILALALAAASSTLVAPAFADEWRHDG
                     DGAREHEWREHDEHERRPDIEHGYRWYAPGYAYNYGYVAPSYVPAPTY"
BASE COUNT         2567 a         4899 c         5097 g         2412 t
ORIGIN      
        1 tcgatccgcg gcttcaggca gcagccgagg ccgcggtcga ggcgacactc tcgcgccagg
       61 gcgagaaggc gggcgtcgag caggcggcgc tggtggcgat gtctcccgat ggcgcggtgc
      121 gggccatggt tgggggacgc aactacggcg aaagccagtt caaccgcgtg acgcaggcgc
      181 tgcgccagcc gggctcggcg ttcaagcctt tcgtctatct tgccgccttc gagcacggcc
      241 tcggtcccaa tgaccgcttc gtcgaccagc cgatccggat cggcaactgg cagccgcaca
      301 attacgaagg cacttatcgc ggcgagatca cggtcaccga cgcgctcgcc cattcggtga
      361 atacggtggc ggcgcaagtg ctgcagcgcg tcggcgtcga caacgtcatc gcgaccgccc
      421 atcgcctcgg catcacctcg gagctcgcgc gcgatccgag cctcgcactc ggcaccagcg
      481 aggtgtcgct gatcgatctc accagcgcct attgcgcctt cgcctcgggc ggcgtcggcg
      541 cgctgcccta cggcatcttc gagatccgcg accgcgacgg gacggtgctc tacaagcgcc
      601 acggcgatgg cggcgcccgg gtcatctcgc ccgagctcgc cggcatgatg aacgacatgc
      661 tggccgaggt cctgagccgc ggcaccggtc gcgccgcggc gctcgaccgg ccggcggccg
      721 gcaagacggg caccactcag gatttccgcg acgcctggtt cgtcggctat acggccgatc
      781 tggtcgccgg cgtctggctc ggcaacgacg acgacacgcc gaccaaacac gtcatcggcg
      841 gcactttgcc ggcacgcacc tggcgggcct tcatggtagc cgcaagcgaa ggcaccgcgg
      901 cgcggccgct acccagcgca ccgcaggtgg cgctcacggg ttcctcttcg ggcggctggc
      961 tctcgcgtct cctccacaac atattcggca gcgacagccg cgcgacgccg cagcaccgcg
     1021 atccttccgc cccccggccc aacgggtagc ggcattgcat cggcgcttgc ccgcgtcctt
     1081 tccgctctgc cccgccggga gagaggatcc gcgggcctgc acccgaaatt ccgccaaata
     1141 ggcgcgcgat catgtcggtg cgcaatctcg acaagctgat gaagccgaag tcgctggcct
     1201 tgatcggcgc gacgccgcgg accggcgcgg tcggtgcggt ggtgctacgc aatctgcgcc
     1261 gcgccggctt tcaaggaccg ctgatgctgg tcaatcccaa gcatcacgcg cttgacggca
     1321 tgcccgtcta tcgcgacgtc caaagcctgc cggagacacc ggacctcgcc gtcatcgtga
     1381 ccccgcctga ggccgttccc ggcctcattg ccacactggg cgcgcgcggt acgcgcgcag
     1441 ccgtcgtcat cacggccggc ttcggtgagc tgggcgagcg cgggcgtgcg cttcagcagg
     1501 cgacgctcga agcggcgcag ccgcatctgc tgcgcctcgt cgggccgaac tgcatcggcg
     1561 tcatggtgcc cggcgccggc ctcgacgcga gcttctccca catcgcgccc aaggccggcg
     1621 atctcgcctt cgtcagccag tccggagcga tgatcacggc cgtgctcgat tgggcggcgc
     1681 cgcgcggcat cggcttctcg catgtggttt ccttgggcga catggccgat gtcgattttg
     1741 gcgacatgct cgactacctg gcggaggacg gcgacacgcg cgccattctc ctctatgtcg
     1801 aagcgatcac ccatgcccgc aaattcatgt cggcggcgcg cgctgcggcg cgcgcgaagc
     1861 cggtgctcgc ggtcaaggtc ggccgctttg ccgaaagcgc ccgcgccgcc gcgtcgcaca
     1921 ccggcgtcct cgccggctcg gatgccgtct atgacgccgc gttccgtcgc gccggcatgc
     1981 tgcgtgtcga caccatggcg gagcttttcg acgccgtgga gacgctggca ctgacacgac
     2041 cgcagcaggg cgagcgcctc gccatcgtca ccaatggcgg cggaccgggc gtgctcgcga
     2101 ccgacgcgct catcgccgcg ggtggccgcc tcgccgagct gtcccccgac atcgtcgcaa
     2161 agctcgatgc ggtgctgccg cgcagctgga gccacgccaa ccccgtcgac atcatcggcg
     2221 acgcgccggg caagcgctat gcggacacgc tacgcatcct gatggcggag ccggcgatcg
     2281 atgccgttct ggcgctcaac gcaccgaccg ccatggcccg accgggcgaa gcggcacaag
     2341 cggtgatcga ggaattcaag gccaccccgc aatcccggct ggtggggcgc aatctgctca
     2401 ccgcatggct tggcgagcac tcggcggcgc cggcaaggcg ccgcttcgcc gaggcgcgga
     2461 tcgccagcta cgagacgccc gacggcgcgg tgcgcggctt catgcatcgc gtccagtacc
     2521 gccgcaatca ggacctgctg atggagacgc cggcggcgcg gcccgacgat ttcacgcccg
     2581 atgccacgac ggcccatcag gcgatcgccc gcacggtcgc cgcgggcggc ggctggctcg
     2641 ctgccgaaga cgccgcagcc gtgctcggcg cctatggcat tccgttgccg gcgagcctcg
     2701 tcgtcgccga tgccgcgggc gcagcggacg ccgcttccgc catcggcttt cctgtcgcgc
     2761 tcaagatccg ttcccccgac ctcacgcata aaagcgatgt cggcggcgtc gttctcaacc
     2821 tcggtacggc agagggtgtg cgcaccgagg caacgctgat gctcgagcgg gtgaaggcag
     2881 cgcagcctcg ggctcggatc gatggtttcc tcgtccaagc gatggtacag cgcccgggcg
     2941 cgctcgagct catcctcggt ctcatcgacg atcccacctt cgggccggtc gtcatgttcg
     3001 gacagggcgg cacggccgtc gagctgctgc acgacacgac gctcgagctg ccgcccctca
     3061 acgccgcgtt ggcgcgcgcg cagatggcgc gcaccggcgt ctggcggctg cttcaaggat
     3121 atcgtggcca accgcccgcg gcgatcgatg ccatcgccga tacgctgatc cgcgtggcgc
     3181 agcttgccgc ggaccacccg gagatccgcg agctcgatat caacccgttg ctggccgacc
     3241 agaaaggcgt gctggccttg gacgcgcgca tcggcgtcgc gccggcgcac atccccggcg
     3301 cggcgcggct cgccatctcg ccttacccga aggagttcga gagcaccgcg acgcttcgcg
     3361 acgggacggt catccgcctc cgtcctgtgc gccccgagga cgagccgctg ctgcaggatc
     3421 tcgcccgtca catgaacgcc gacgatctca ggctacgctt tttcacgccg gtgacgacgc
     3481 tgtcgcacca gctgttggcg cggctgtcgc agatcgacta cgaccgcgcc atggcgctga
     3541 tcgcgcggcc ggccgtcgag gggacggcgc tcgggatcgt gcgctttgcc gcggatcccg
     3601 acaatcgccg cggcgaattc gcggtgtccg tacgcagcga ctggaagggg cgaggcttgg
     3661 gttatctgct gatgacgcgc attatcgagg tcgcccgcca gcgcgacctc ggcgagatct
     3721 tcggcgaggt gctgcgcgag aacggtgcga tgctgcgggt ggctcgcgcc ctcggcttct
     3781 ccgtcacgac ccacccgcag gaggctcatc tcctgcgcct tacgaagccg ctttagaaga
     3841 ggccgatcga cgtctggctc aattgctgcg cacgtggagc acgaggcggc ggtagaaatc
     3901 ggcgaggacc gtggcgccga gtgctgtgaa gatgaacagc agcacgatgg tgagcacgcc
     3961 gttcaggaga aaaagctcgg tcggcggagg cgccagcgga tcggggagcg gcagatgcgc
     4021 gccctcgagg atcagatcga ggagcagcca gacgagcagc agaatcggtg tggtgaccag
     4081 cagcgtcgcc gcgagaaagc gccaggtgtt gccgcgcatg tgccgccagg cgccggcaag
     4141 gcccagcggc tgaccgatga ccaccgccgg aaagaccagc gaacagcggg cgacgacgag
     4201 ggagatggcg agcgcgatga cggcgacgag ggcatcggcg gtgttttccg tgaacagctc
     4261 ggagagcccc gcaatcggat gatccgtgat cgcggcggac agggcatgaa gagcgaccgg
     4321 cgctcccgcc gcgactgcca gcatcaccag atagaacggg agcgaatagc ccaggaatcg
     4381 cgcccacacc gcaagaaagc gccgcagcgg cagcggccga tccgccgcca gcaccaagtg
     4441 gtgccagcgc acggcaaaag cggagaggca cagcatcacg atcacggttt cggcgatgtc
     4501 ggcgcccggc aggctggctg ccgccgccgt atcgtcgggc cagaaatagt cgctgatcag
     4561 tctggggagc agctcgaccg ccagaagaac cagcatcggc agccaagcga cctcaagcac
     4621 gcggccgggc cgcccgaaca cgacgcgata ggccgccgcc gcgacgcggt cgatcggcac
     4681 tttgacccgc cccgattcgc cctcgagcgc cgccattgcc cctccttgga gatcgccgcg
     4741 gaagctagcg gggattcctc ggaggaacaa gcgctctgcc ggcctcgtaa gccctcacgc
     4801 agcgacgaga tcgagcgcgt agccggccgc gcgcaccgtg cggatgacgt cggggcggct
     4861 gccgttgttg agcgccttgc gcaggcgacg gatgtgcacg tcgacggtgc gcagttcgac
     4921 ttcggcatcg cgtccccaga cgccgtccag cagctgctcg cgcgagaaga cgcggccagg
     4981 acgctcgaga aggaagcgca gcaggcggaa ttcggtcgga cccagatgga ccgccgaatc
     5041 gccacgcgtc acgcgatggg cagagagatc catggcgatg tcgccgaagc gcagggtctc
     5101 ggccgacgcg gccggccgca cgcggcggat gaccgccctt aaccgcgcga tgagctcgcc
     5161 cgggctgaag ggttttacga cataatcgtc ggcgccgctg tcgagaccgc gcacgcggtc
     5221 gccttcctcg ccacgcgccg tcaacatgat gaccggcagg tcccgccacg ccggcgcccg
     5281 acggatctgg cggcagacct cgagacccga gatgcgcggc agcatccagt cgaggagcac
     5341 cgcatcgggc ttgctctcgc gcagctgcag caaggcctct tcgccgtcat gcgcctcgtt
     5401 cacggcgaat ccctcgcgct cgaggttata gcgcaagagc gtgacgagcg ccggctcgtc
     5461 ctcaaccacc agtaccagcg gccgcattac tcatcctcct gatggtcgtc atgttcgatg
     5521 gggggcggcg cgccacgcac cacggcgaga ctcgagcggt cgccctttgg ccgcgtctgg
     5581 gcaagcggcg tgccatgcac gaggaaataa agcgtctcgg cgatgttggt cgtgtggtcg
     5641 ccgatgcgct cgagattctt ggcgatgaag aggagatggg tgcaggcggt gatgttgcgc
     5701 gggtcctcga tcatgtaggt caggagctcg cggaacagac tcgtatacat ctcgtccagt
     5761 tcttcatcgc gcaaccacac ggcgagcgcc ttgtcggcgt cgcgctcgac ataagcgtcg
     5821 aggatgtcct tgatcatcgc ctggccgagc ctgcccatgc gcggaatggc atagaccggc
     5881 cgcaccggcg gcgtctggtt gagcgcgatc gcgcgtttgg ccacattggc ggcgtagtcg
     5941 ccgacgcgct cgaggtcgct cgcgatcttg agcgcggcga tgctctggcg cagatcgcgc
     6001 gccatcggct ggcgcagcgc gagaagccgc atgaccaggc tctcggtgtc gcgctcgagc
     6061 gcgtcgatcc gcgcatcgtt ctcgatcacc tcgctggcga aatcgctgtc gcgcttcacc
     6121 actgcgtcga tggcggcagc gagctggctc tcggcgagcc cgcccatctg cgtgatgatg
     6181 ttgtcgaggc gttgcagctc ttcgtcatag ctcttgatga tatgctcgga aaccaatgcg
     6241 tcctccatca gccgaagcgg ccggtgatgt agtcctcggt ccgtttctcc cggggatggg
     6301 tgaaaagcgc ctccgtctcg ctgaactcga cgagctcacc gagatacatg aaggcggtgt
     6361 agtcggagct gcgcgccgcc tgctgcatgt tgtgcgtcac aatggcgatg caatagtcgg
     6421 atttgagctc ggcgatgagt tcctcgatgc gctgcgtcga gatcggatcg agcgccgagg
     6481 cgggctcgtc gaggagcagg atctccggct tgacggcgac ggcgcgcgct atgcagagac
     6541 gctgctgctg gccgcccgag agcgagagcc cgctctggcg gagcttgtcc ttgacctctt
     6601 cccagagcgc cgcgcgcctc agcgcctctt cgacgcgctc gtcgagcgcg ccgggcgtga
     6661 gccgctcgta gaggcggatg ccgaaagcga cattgtcgta aatcgacatc gggaagggcg
     6721 tcggcttctg gaataccatg ccgatctttg cgcgcagcag gttcagatcc tgtccgggct
     6781 cgaggatgtt gcggccatcg accagcacct cgccggtggc gcgctgtccc ggatagagct
     6841 gatagatgcg gttcaggatg cggagcagcg tcgacttgcc gcatcccgat gggccgatga
     6901 aggcggtgac acgcttgtcg tgcaggacga ggttgatgcc cttcagcgcc tcgaattgct
     6961 tgtaatagaa acgcacgtcc ttgatcacga ccttcggcgc ggtctgcgca gccgccaccg
     7021 gcccgcgcgg cccgacaatt ggcctgccaa gcgtcggcgg gcgtgcggct tccgacgcgg
     7081 tcaatgcgaa atcggcggtc atggcgtgcg gttgctccag ctggcgagaa ggcgtgcaac
     7141 aatgttcaac agcaggatcg atagcgttat cagcatcgcg ccgccccacg caagatcgac
     7201 ccagttgtca taggggctgt tggcaaactg gaagatgacg acggggaggc tggccatctc
     7261 ccggttcatg tcgagcgagg tgaactggtt gctgaccacg gtgaaaagca gcggcgcggt
     7321 ctcgccggcg atccgcgcca ccgcgagcat gacgccggtc agcatcccct gaagggccgc
     7381 ccggtaggcg accatgacga tgactttcca ttgcggcgcg cccagcgcga cggccgcctc
     7441 gcgcaggctg ccgggcacga ggccgagcat gttctcggtg gtgcgaacca cgaccggcac
     7501 gacgatgatg gcgagcgcgg ccgatcccgc ccaggcggag aagccgtgca tgggcaccac
     7561 gatgagccca tagacgaaga cgccgatgat gatcgacggg gcggagagca ggatatcgtt
     7621 gacgaagcgc accgcgggcg cgatccggct gccgcggcca aattcggcga gatacgtgcc
     7681 ggtaagtatg ccgacgggtg tgccgaccag cgtcgcgagt ccggtcatga agacgctgcc
     7741 gatgatggcg ttggccatgc cgcccccggt gctgccgggt cccggggtct ctttggtgaa
     7801 gaagtccacg ctgagggccg gtatgcccct ccacagcaac tcgcccagaa tgagaaagag
     7861 aacggcgaga ccaatcccgg tcgcggtcca ggagagggtc agatagacgg cgttgacggc
     7921 gcgccggcgc gcgtagaggc cgcccgattg catcacttgg ccgcccgctg ctcgagttcc
     7981 agcagcatca gcttggcggc ggcgagcacg aggaaggtga tgacgaagag gatgaggccc
     8041 agcgcgaaca gcgccgagac gtacatgtcg tccttggcct cggcaaattg attggcgagt
     8101 gtcgccgaga tggtggtgcc gggcgagagg agggatggct ggaggcggtt ggcattgccg
     8161 atgacgaagg tgacggccat cgtctcgccg agcgcgcggc ccagggccag catgatgccg
     8221 ccaatgacgc cggtgcgggt gtagggcaat acgatgtcgc gcacgacctc cgtggtggtg
     8281 gcgccgacgc cataggcgct ttcacgcagc atgggcggca cggtcaggaa gacgtcgcgc
     8341 gtcaccgcgg cgacgaaggg cagcaccatg aaggcaagaa tgataccggc cgtaagcaca
     8401 cccagaccga agggtgggcc gctgaacagc gcgccaatga caggcacctg gccaaggctg
     8461 tcggtgagcg ccggctggac ataggcggcc atgatcgggc gcatgacgaa aaaaccccag
     8521 atgccgtaga tgatgctggg gattcccgcc agcagctcga tcagcgtgcc gatcgggcgg
     8581 cggaacggcg gcggacagag ctcggtcaag aagaccgcga tgccgaagcc gagcggcacg
     8641 gcgatcacca tggcgatgag cgaggtgacc agcgtcccat aaattgccgg aagcgccccg
     8701 aaattctcag taacggggtt ccaggactga ttgatgagga agcgccagcc gaacttctcg
     8761 aacgccggcg ccgcgccgac ggcgagcgcc aggaggatgg cgccgagcag gacgagaacg
     8821 ccgacggcaa agagctgcgt cgcgctgcgg aaagcggcgt cctggaggtg ctgggtgtcg
     8881 agccggcggc gagtcgcggc cgtcagtgcg cttgccatac aggcttgccg ctagagtcct
     8941 tgatgttggc cgcccaggag gcctcgacca tcttgatcac gccctccggc agcggcacgt
     9001 agtcgagttc ctcggccatc gtcgagccgt tctgatagga ccaggcgaag aaatcgagga
     9061 cctgcttggc gacctccggc ttgtcctgaa ccttgtggat caagatgaag gtggcgccgg
     9121 tgatcggcca gctctccttg cccgactggt ccgtcagcag cagatagaag ccaggcgcgt
     9181 tccgccaatc cgcattggcg gccgccgtct ggaaactctt gctgtcgggc gcaacggtgc
     9241 tcccttcctt atttatcagc ttggtatagg caagcttctt ctgcagcgcg aaggcgtatt
     9301 cgacatagcc gatggccccc ggcgtggtcg ccgcgagcgc cgagatgcct tcgtttccct
     9361 tccccccgac gcccgtcggc cactcgaccg aagtgttctc gccgaccttg cttttgaatt
     9421 cggggctgac cttcgagaga tagtttgtga agatgaaatt ggtgccggag ccgtcggagc
     9481 ggtagagcgg cgcgatcgcg gtcgccggca gcttggcgcc aggattgagc ttcttgatcg
     9541 ccggatcgtc ccatttggcg atcttgccca gatagatatc ggcgattgtc gggccatcga
     9601 gcgtgagatc gcccggcttg acattcggca ggttgacgac gaggacgacg ccgcccatga
     9661 ccattggcca ctgggtcagg tcggcctttt caagctcttc gggcttcaaa ggcatgtcgg
     9721 aagccccgaa gtcgacggtc ttcgcattga tctgcttgat tccgccgccc gagccgatcg
     9781 actggtagtt caggctgacc ccggtcttgg ccttataggc ctcggcccat ttggcataga
     9841 tcggataggg gaaggttgcg ccggcgccgg aaatctcgat ggcgctggcc ggcgccgata
     9901 ggacagcagc gccgaggagc gcgatggcgc cgagccaagc cgtcgttttg ctgcgaatct
     9961 gcacgggtaa tgcctcctcg gatcgttttt tgatcgaagc tcggccgctc ctgccccctt
    10021 cggagaagct gccacgcgct ttcgcccccc ctggctaccg aggtggagtt acacgagtac
    10081 gacattttaa ttacaatttt atgacaattt catggggcgg acatcggcag gggcaaatag
    10141 accgtaaagg ttgagccttc tccgatctcg ctgtcgattt cgagagcgcc tcgatgccgg
    10201 ttgacgatgt gcttgacgat ggcgaggcca agaccggtgc cgcccagctc gcgcgaccgc
    10261 gccgggtcga cccggtaaaa gcgctccgtc agccgcggca gatgctcgcg cgcgatgccg
    10321 tcgccgtggt cgcgcacggc aatcgcgagg gccgccggct tctcgcccgc ccgggcgccg
    10381 ggcaggaaac gcgaggaagg ccgggccgtg acctcgatcg gcgtgcccgg ccggctgtat
    10441 ttgatggcgt tgtcgagcag attctgaaag acctgggcga gctcgtcggg gtcgccttgc
    10501 acctcgggaa ggccctccgg cagctcgagg atgagctgca tattgcgggc ctcggcgcgc
    10561 tgctcgagga ggtcgccgac ggcatggagg acgcggccga tatgcacccg gtccgtcggc
    10621 agccggtgct cgttcatctc gatgcgcgac agcgacaaga gatcgtcgac gagacgggcc
    10681 atgcgctgcg cttgctcggc catgatggcg aggaagcggc tgcgcgccgc gccgtcgtcg
    10741 cgcgccgggc cgcggagcgt ctcgatgaaa ccggtcagcg aggcgagcgg cgtccggagc
    10801 tcatgactcg catttgcggc gaagtcggcg cgcagccgct cgctgcgctt ggctgcggtg
    10861 agatcgtgca gcgtcagcac cgccgcggcg ccttcgggcg tatgcggccg gagcggtgcg
    10921 agcctcgccg acaggtgacg gtcgacgggg gtggtgacgt cgaagtccac aacgcgcgac
    10981 gccccgccgc gcagcactgc gtcggcggcg tccagaaccg ccggatggcg gatcgcgacg
    11041 gcaagatcgc gctccacgag ccgcatgccc aggagctcgt tggcggcggc attggcacgc
    11101 aggatccgcc ggcgatcgtc gagcaagatc agcggatcgg ggagcgccgc cagcacggcc
    11161 tgagcggcgc cgagctcgac agcgctggcg gcgagcttct ggcgcgatgc gcgtgcccaa
    11221 cgaccgacgg caagccacaa ctcgcgcacc gccggactta gcgttgccac atcgggagcc
    11281 gccccgtcgc cggctgccag cgcgtcgacc gccgcctgca ccttggcgat gcccatgacc
    11341 aacgggcgca gcagcacgag aacagcggca tagaccgcaa gcgccgcaag cgctgcgccg
    11401 ctcgcgccga tgaagcctcc cgccgcgatg accccaagga cgacaaaggc cgggctcgac
    11461 agaatcaccg cgccgagcag ccatcgcgcg gtggcactca tcagcaaccc gccgggggcc
    11521 aactctcccg ccgagggaga aggtctgggg gagagcgtcg tccggcgcga tgatcgccga
    11581 tgaacatctg gacctctctc tcgcggtcaa cgctccctgt cgccgcgctg cgctatgccc
    11641 tcttactcga tcgcttcgtg ggcggcgagg gcggcgagat tgacgagatc cgagacggtg
    11701 gcacccattt cgacgatctg cgccggccgc tcgagcccca tcagcagagg acccacgcgc
    11761 gttccgccgc cgagctgctc caagagctgc gacgcgatgt tggcgctgtt gaggccgggc
    11821 atgatcagga cattggccgg accggtgagc cggcaaaagg gatagcgctc gcgcatcagc
    11881 gccaggttga gcgcaacctc cgcgctcatc tcgccgtcat attcgaaatc gacctgccgc
    11941 gcatcgagga gctcgaccgc ctcatgcgcg cgccggcccg cctcgccggg cggattgccg
    12001 aaggtcgaat atgaaaggag cgcgacacgc ggctgctgtc ccatcatctg tcgcgcgcgg
    12061 tgtgccgact gaacggcgat gtcggcgagc tcctcggcgt cgggaagcgc gtgctcggtg
    12121 gtatcggcga tgaaaacgga gcgtccgcgc gacagcagca gcgcgtagcc gcagatgcgg
    12181 tgtccgggct tcgcccggat cacgcgcgtc acctcctcga atgcagtgaa atagctgcgc
    12241 gtcgcaccgg tcacgagcgc atcggcatcg ccgcaggcga ccatgcaggc agcgaaggta
    12301 ttgcgatcct gattgaccat ccgctggcag tcgcgataga gcgcgccctc gcgctgcaga
    12361 cggccgtaga ggaattgcgt gtaggtcttg ttggcgtgcg acaggcgcgc attgtggatc
    12421 tcgagctcgc ccgcggcggc caggcccatc tggcgcagcc gctcgcggat acgctcctcg
    12481 cggccgatca gtacgggcgt gccatagccg ctgtcgcgga aggagagcgc ggcgcgaatc
    12541 gtcttctctt cctcgccctc ggcgaagacg acgcgcttgg gtgaagcgcg cacggcatcg
    12601 aaggtgcgct gcagggtggc ggccgtcggg tcgagccggc cgcgcagggt tgcgcgatag
    12661 gcatcgagat tggcgatcgg acggcgcgcc acgcccgaat ccatggccgc cttggccacg
    12721 gccgaaggca cggcccagat gagacgtgga tcgaaaggtg tcggcacgat gtagtcgggc
    12781 ccatattgga gacgctggcc cgaataagcc gccgccacct cgtccggtac gtcctcgcgc
    12841 gcgagtttcg ccagcgcatg cgcggccgcg atcttcatcg cttcattgat ggtggaggcg
    12901 cgcacatcga gggcaccgcg gaaaatgtag ggaaagccca agacattgtt gatctggttc
    12961 gggtagtccg agcgtccggt gcagatgatg gcgtcaggcc ggacggcgcg gacgtcctcc
    13021 ggtgtaattt cgggatcggg gttcgccatg gcggcgatca tgggccgcgg cgccatcccc
    13081 ttcaccatgg cctggctgac ggcgcccttg actgaaaggc cgaagaacac atcggctccg
    13141 tcgagcgcct cggtcagcgt ccggcgcttg gtattgatcg catgcgccga tttccactgg
    13201 ttcatgcctt cgctgcgccc ctggtagacg acgcccttgg tgtcgcagag aatgacattg
    13261 tcgtgcgcga gccccatcga tttcaggagt tcgacgcagg cgatcgaggc ggcgcccgca
    13321 ccattcacca ccatcttgat ggtcttgaga tcacgtccgg tgagatcgag cgcgttcaag
    13381 agcgcggcgg tggcgatgat agcggtgccg tgctggtcgt cgtggaatac cggaatgtcg
    13441 aggagttcgc gcaggcgctg ttcgatgatg aagcattcgg gcgccttgat gtcctcgaga
    13501 ttgatgccgc cgaaggaggg gtggagatag cggacgcagt tgacgaaggc gtcgacatcc
    13561 ttggtgtcga tctcgagatc gaccccgtcg atcccggcga agcgcttgaa gaggaccgcc
    13621 ttgccctcca tcacgggctt ggccgcgagc gcccccaggt cgcccagtcc caggacggcg
    13681 gtgccattcg agatcaccgc caccagattg ccctttgccg tatagtcgta agcgagcgac
    13741 gcgtcgcgcg caatgtggag acagggaaag gccacaccgg gcgagtaggc gagcgatagg
    13801 tcgtgctgcg tggtgagcgc cttggtgggc gtgacctcga gtttgcccgg tcgtccctcg
    13861 gcgtggaatt gaagggcttc ctcctcggtg acccgggcgt cgggcttatc catggacgac
    13921 aaggcgattt cctcgtgctg gaaggaaact ccaaggttcc cttctccttt atggcggggg
    13981 gcggcagtct aaagagcgcc gctccgcccg tcaaagcacg ccggcgggtg gccaacgatc
    14041 cacctcgccc ctcgcgggcg gggacgggag aaaatccccg accgggtttc gtcaggcggg
    14101 catcgccatg cgggcacgcc attcgaccgc ttcgaagccg gtcacgtccg taaggaatcg
    14161 ctggccgctc ggatcgataa agaggtggca gcggaagcga taccagacgc cgtcgaggac
    14221 gaggatgcct tcatagtcgt tggcgatccc gccatcctct tcctggagcg actggacgag
    14281 acgcggcacg atggtcgcgc gctccgcgag aacgacgctg agccgcccca tggcgcagcc
    14341 ctcgcaaagc acgtcttcaa gccgcaaccg gtaggtcgcc aggaggaagg catcgaccgt
    14401 gtcgaaggcg tgcatgagat gagctcctga tcgcaatcgg ggccatccat tatggcggcg
    14461 gcctccgaac atatcaagaa catttgccca cccaggcttg ctcgcctggg ggccaagatg
    14521 acctggcgtt gagtccttcg atgttctttt tgcgcccccg ttccgttcag gggccgagcc
    14581 aaataatgag ccgccgaagc cgcgtcgcag gtgaaacctt tgtcatgggc acggagaaag
    14641 gtgagcttgc ttatcatcta gcataagtcc ctgtagcctc acggaaagga gaggcccatg
    14701 aagacccgca agacggcgac ccccgccctt cgccgcatgc tccacggcag catcctggcg
    14761 ctcgcgcttg ccgcggctag cagcacgctc gtcgccccgg cctttgccga tgaatggcgc
    14821 catgacggcg atggtgcccg cgagcacgaa tggcgcgagc atgacgagca cgagcgacgc
    14881 cccgacatcg agcacggcta tcgctggtac gcccctggct acgcctacaa ttacggctat
    14941 gtcgcgccga gctatgtgcc ggctccgacc tacag
//
LOCUS       DASYNS010000360         3450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1493293, whole genome shotgun sequence.
ACCESSION   DASYNS010000360 DASYNS010000000
VERSION     DASYNS010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493293"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VGP50_11255"
     CDS             <1..353
                     /locus_tag="VGP50_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028001.1"
                     /translation="ALAYAILALAAREAGDGKASWLWLLGYFVVITVAELYLSPIGLS
                     LVTKVAPASILSLMMGLWLATSFVGNLMAGWLGGFWSGMDRPDFFLMIAGVSALGAAA
                     IWSVRSTLRSALER"
     gene            472..2271
                     /locus_tag="VGP50_11260"
     CDS             472..2271
                     /locus_tag="VGP50_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV8028002.1"
                     /translation="MDYQTCPNLAAMFFDWAAERGERPFLWAKRDGAYHAITWTEATR
                     RVSMLARGLRALGVKAGDRVALIAENRPEWTIADLAIMTAGAITVPAYTTHTAEDFRH
                     VLANSGARGVIVSTASLANRVMPAADQVSTIASIITMEPLTRGQLSHAEIYLWDDVMA
                     RGAALPDEVASWVKAMGRDDIACLIYTSGTGGVPKGVMTTHANILANCYGAFRLLETI
                     GLGDDVFLCFLPLSHAYEHTAGMMFAISVGAEIYFAERAETLAANMPEARPTIMTAVP
                     RLYETLHQRIISGIERQGGFRARLFRAAVDIGRKRYDHPETLTWRDRVLDVLLNLLVR
                     RRIAARFGGRLKAMVAGGAPLNPEIGRFFLALGVPLLQGYGQTEASPVISVNPPKRIK
                     IDTVGPPLEGVEVRIGEDGEILARGPMVMKGYWNDPEATARALADGWLHTGDIGEIDS
                     DGYLRITDRKRDFIKNSGGDMVSPARVEGYLSLQPDIGQVMVHGDRRPYLVAVIVPAA
                     ELLERRAGGRPSAAASDGADLIVDPELHKVLAAAVARVNAELKPIERVRRFIIAAEPF
                     TVANGRMTPTLKIKRHAIRHAYGAALQALYEAK"
     gene            2268..2813
                     /locus_tag="VGP50_11265"
     CDS             2268..2813
                     /locus_tag="VGP50_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028003.1"
                     /translation="MTEVARRVLLFALLGVGSGAAAATAFELADPESFFYVFLFYLAP
                     GLVFGVALGPTLWRCRVWRAAGAGFYAIAAALAHGCAMFTAIVLDDRLQRVLNGHIPV
                     LIVSGVIAGALGASLIGGVTRLLAPVPRWPVLAAPGALLGALLPIAVEGDTPGFFVFY
                     VLWQAGYAATLALILPRIETV"
     gene            complement(2810..>3450)
                     /locus_tag="VGP50_11270"
     CDS             complement(2810..>3450)
                     /locus_tag="VGP50_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV8028004.1"
                     /translation="PRLAHLVFASSSSVYGGNTKLPFAISDPVDTPQSLYAASKKADE
                     LMTYCYAHLYRLPSTGLRFFTVYGPWGRPDMAAWLFTDAILKGEPIRVFNNGAMRRDF
                     TYIDDIVAGVLAALDHPPGDSGKEPPYRIYNLGNHRSEELKRFIAVLEQATGRQAIIE
                     LAPMQPGDVSATYADISETTRDFGFVPKTTIDEGIPRFVEWYRRYHRIERGP"
BASE COUNT          519 a         1153 c         1151 g          627 t
ORIGIN      
        1 gcgcgctcgc gtatgcgatc ctggcgctcg ctgcccgcga ggcgggagac ggcaaagcga
       61 gctggttgtg gctcctcggc tatttcgtgg tcatcaccgt ggccgagctc tacctctcgc
      121 cgatcggcct gtcgctcgtc accaaggtcg ctccggcctc cattctctcg ctgatgatgg
      181 gtctgtggct ggcgacgagc ttcgtcggca acctcatggc ggggtggctc ggcggtttct
      241 ggagcggtat ggaccgaccg gacttcttcc tgatgatcgc cggggtctcc gcgctgggcg
      301 ccgccgcgat ctggtccgtc cgctcgaccc tccgctcggc cctcgagcga tagagcggcg
      361 ctacgcaaga agtcgtgcgc cgggctattt cgccacgccg tcaaacgccc tacaacgccg
      421 ccaaagacac ggccgaccgg gcgggtcagg cggtgtcgag cagggaggcg catggattac
      481 caaacctgcc ccaacctcgc cgccatgttt ttcgactggg cggccgagcg cggcgagcgg
      541 ccgttcctct gggcgaagcg cgacggcgcc tatcacgcga tcacctggac cgaggcgacg
      601 cgacgcgtga gcatgctggc gcggggtttg cgcgcgctcg gcgtcaaagc cggcgaccgt
      661 gtcgctctca ttgccgagaa ccggcccgaa tggacgatcg ccgatctcgc catcatgacg
      721 gcgggcgcca tcaccgtgcc ggcctacacg acccacacgg ccgaggattt tcgtcacgtt
      781 ctcgccaaca gcggcgcgcg cggcgtgatc gtctcgacgg cgagcctggc gaatcgcgtg
      841 atgcccgctg ccgaccaggt ctcgaccatc gccagcatca tcaccatgga gccgctgacc
      901 cgagggcagc tctcccacgc cgagatctat ctctgggacg atgtgatggc gcgcggcgcg
      961 gcgctgcccg acgaggtcgc gtcctgggtc aaggcgatgg gccgcgacga tatcgcctgc
     1021 ctcatctaca cctcgggcac cggcggcgtg cccaagggag tgatgacgac gcacgccaac
     1081 attctcgcga attgctatgg cgccttccgt ctcctcgaga cgatcgggct cggcgacgat
     1141 gtgttcctgt gcttcctgcc gctctctcac gcctatgagc acacggcggg catgatgttc
     1201 gcgatctccg tcggggccga gatctatttc gccgaacgcg ccgagacgct cgccgccaat
     1261 atgccggagg cgcgcccgac catcatgacg gcggtgccgc gcctctacga gacgctgcat
     1321 cagcggatca tcagcggtat cgagcgccag ggcggctttc gcgcgcggct cttccgcgct
     1381 gccgtcgaca tcggccgcaa gcgctatgac catcccgaaa ctcttacctg gcgcgaccgc
     1441 gtcctcgacg tcttgctcaa tctcctggtc cgacgccgca tcgccgcgcg cttcggcggg
     1501 cggctcaagg cgatggtggc agggggcgcg ccgctcaatc cggagatcgg gcgcttcttc
     1561 ctcgccttgg gcgtgccgtt gctgcagggc tatggccaga cggaggcatc gccggtcatc
     1621 tcggtcaatc cgcccaagcg catcaaaatc gacacggtgg gcccgccgct cgaaggcgtc
     1681 gaggtcagaa tcggcgagga cggcgagatc ctggcgcgcg gtccgatggt gatgaagggc
     1741 tattggaatg atcccgaggc gaccgcccgg gcgctcgccg atggctggct ccataccggc
     1801 gatatcggcg agatcgacag cgacggctat ctgcgcatca ccgacaggaa gcgcgacttc
     1861 atcaagaatt ccggcggcga catggtgtcg ccggcgcgcg tcgaaggcta tctctcgctg
     1921 caacccgaca tcggccaggt catggtgcat ggcgaccggc gcccgtacct ggttgcggtg
     1981 atcgtccccg ctgccgagct tctcgagcgc cgcgccggcg gccggcccag cgccgccgcg
     2041 tccgacggcg ccgatctcat cgtcgatccc gagctgcaca aggtgctcgc cgcggcggtg
     2101 gcgcgcgtca atgccgagct caagccgatc gagcgcgtgc gccgcttcat cattgcggcc
     2161 gagcccttca ccgtcgccaa cgggcggatg acgccgaccc tcaagatcaa gcgccatgcc
     2221 atccgccacg cctatggcgc ggcgctgcaa gcgctctacg aagcgaaatg acggaggtgg
     2281 cccgacgcgt tctcctgttt gcgctgctcg gcgtcggttc gggcgcggct gccgcaacgg
     2341 ctttcgagct cgccgatccg gagagcttct tctatgtctt cttattctat ctcgcgcccg
     2401 gactggtctt cggcgttgct ctcgggccca ccctatggcg atgtcgtgta tggcgagcgg
     2461 caggcgcggg cttttatgcg atcgctgcgg cgctagcgca tggctgtgcc atgttcacgg
     2521 caatcgtcct agacgaccgt cttcagcgcg ttctcaatgg tcacatcccg gtgctgatcg
     2581 tctccggcgt gatcgcgggc gcattgggcg ccagtctgat cggcggagtc acccgcctcc
     2641 tggcgcctgt ccctcgctgg ccggtgctcg ctgccccggg cgcgctcctg ggcgcgctgc
     2701 tgccgatcgc ggtggaaggc gatacacccg gcttcttcgt cttctacgtg ttgtggcaag
     2761 ccggctatgc cgcaaccctc gcgctgatcc ttccgcggat cgagacggtc taaggtccgc
     2821 gctcgattcg atggtatcgt cgataccatt cgacaaagcg cggaatgccc tcgtcgatcg
     2881 tcgtcttcgg cacgaatccg aaatcgcgcg tcgtctcgct gatgtcggcg taagtcgcgg
     2941 acacgtcgcc cggctgcatc ggcgcgagct cgatgatcgc ttgtcgcccg gtcgcctgct
     3001 cgagcacggc gatgaagcgc ttgagctcct cgctccggtg attgccgaga ttgtagatcc
     3061 gatagggcgg ctccttgccg gaatcgccgg gcggatggtc gagggccgcg agcacgccgg
     3121 cgacgatgtc gtcgatgtag gtgaagtcgc ggcgcatcgc gccattgttg aagacgcgga
     3181 tcggctctcc cttgaggatc gcgtcggtga agagccaggc cgccatgtcg ggccgccccc
     3241 aggggccata gacggtgaag aagcgcaagc ccgtgctcgg caggcggtag agatgggcgt
     3301 agcaataggt cattagctcg tcggccttct tgctggcggc gtagagcgac tgcggcgtgt
     3361 cgaccggatc gctgatggcg aagggtagct tggtgttgcc gccatagacg gaggacgagc
     3421 tggcgaagac gagatgggcg aggcgcggca
//
LOCUS       DASYNS010000361         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2585267, whole genome shotgun sequence.
ACCESSION   DASYNS010000361 DASYNS010000000
VERSION     DASYNS010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2585267"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            459..1967
                     /gene="glgA"
                     /locus_tag="VGP50_11275"
     CDS             459..1967
                     /gene="glgA"
                     /locus_tag="VGP50_11275"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV8028005.1"
                     /translation="MRVLFVSSEIYPLAKTGGLADVSAALPNALARLGVEMHLLMPGY
                     PRAIDAAVQKSVAVALDDFMGAGPLRLIAGCIPETGLPLWLIDCPALFRRPGGLYQDE
                     YGQNWPDNALRFAVLNHIAAQLSLGQLLPDWRADIVHANDWHAGLVPAILAGTPGQRP
                     ASVFTMHNLAYQGVFPAAVYPQLGLPADAFSADGVEFYGQVSFLKAGIRYSDRLTTVS
                     PTYAREILTPEYGFGLDGLLRHRAADLVGILNGADYRIWDPALDGNLAANFDAKHIAL
                     KRVCKAALQDEMGLDVAPEAPLFVHVSRIAEQKMADVVAEVLPALVEKGAQCALLGDG
                     ERGLEARFVAAARQHPGRIATRIGYEEPLAHRFLASGDVLLHPARFEPCGLTQLYAMR
                     YGTLPVVRRTGGLRDTVVDADDQGLRQGIATGFAFESATAADLLFATERAIALYRQPL
                     AWRKVQRQAMAQNFDWSESACRYLALYRDLVPEPALISDPVIVEPMRQEKAG"
BASE COUNT          315 a          708 c          660 g          350 t
ORIGIN      
        1 ggcgttctct gaaaagaggg cgaaattcac gcccttgccg ttccaggtcg cgccgagcgg
       61 aaacggctct cccggccaaa cacgggtccg ctgccctttc gtcatgcagg tcctatgcgt
      121 ctaggcgttt cgcgggagag cagccccggt cgtgcagtgc catcgagagc gcgcccggga
      181 ctggggcccg tcctccgcgc gcagcaccct atactggaaa cgcctctttg ggaccagggc
      241 gggacgccgc ggctcggcgc gctcgccgct tcccgcgacc cgcggctgca atgcattcct
      301 gtcatgatca gaaccgggat cgacggggca gcacaagacc accacatcgc cctcgatttg
      361 catcaacgct ccacgcgtcc gtccggcgga ttttcccatg agcggctacc cgccgctctt
      421 cgcggcccgc gacaaaaagc gcggcagggg agatagtctt gcgtgtcctc ttcgtatcct
      481 cggagatcta tcccctcgcc aagaccggcg gccttgccga tgtcagcgcg gcgctgccga
      541 acgcgctggc caggttgggc gtcgagatgc atcttcttat gccgggctat ccccgagcga
      601 tcgacgccgc ggtgcagaaa tcggtggccg tcgcgctcga cgatttcatg ggggcgggac
      661 cgctgcgcct catcgcgggc tgcatccccg agaccggcct ccctctctgg ctcattgatt
      721 gcccggcgct gtttcgccgg ccgggaggtc tctaccagga cgaatatggc cagaattggc
      781 cggacaatgc gctgcgcttc gccgtgttga accatatcgc ggcgcagctg tcgctcggcc
      841 aattgctgcc ggactggcgc gccgacatcg tccatgccaa tgactggcat gctgggctag
      901 tgccggcgat tctggccgga accccgggac agagacccgc gagcgtcttc acgatgcaca
      961 atctcgccta tcaaggcgtc tttccggccg cggtctatcc ccagcttggg cttcccgccg
     1021 atgctttcag cgccgacggc gtcgaattct atggccaggt ttcctttctg aaggccggca
     1081 tccgctacag cgaccgcctg accaccgtga gtccgaccta cgcgcgcgag atcctcaccc
     1141 ccgaatacgg cttcggcctc gacggcctgc tgcgacatcg cgcggcggat ctcgtcggca
     1201 tcctcaatgg cgcggactac cgcatctggg acccggcgct cgacggcaat ctcgccgcca
     1261 acttcgatgc gaagcacatc gccctgaagc gcgtgtgcaa ggccgcgctg caggacgaga
     1321 tgggcctcga cgtcgctccg gaggcgccgc tcttcgtcca tgtgagccgg atcgcggagc
     1381 agaagatggc ggacgtggtc gccgaggtgc tgccggcgct cgtcgagaaa ggcgcgcaat
     1441 gcgcgctgct cggcgacggc gagcgcggcc tcgaggcgcg cttcgtggcc gccgcgcggc
     1501 agcatccggg ccgcatcgcg acgcgcatcg gctatgagga gccgctggcg catcgcttcc
     1561 tggcgagcgg cgacgtcctg cttcaccccg cgcgcttcga gccctgcggg ctgacgcagc
     1621 tctatgcgat gcgctatggc accttgcccg tcgtccggcg caccggcgga ctccgcgaca
     1681 ccgtcgtcga tgccgacgac caggggcttc gtcagggaat cgccacgggc ttcgccttcg
     1741 agagcgcgac ggccgccgac ctgctgttcg cgaccgagcg ggcgatcgcg ctctaccggc
     1801 agccgctcgc ctggcgcaag gtgcagcggc aggcgatggc ccagaatttc gactggtcgg
     1861 aatcggcctg ccgctacctg gcgctctatc gggatctcgt gcccgagccc gccttgatat
     1921 ccgatcccgt catcgtcgag ccgatgcgcc aggaaaaggc aggataggag aggaggcctg
     1981 gatgacaccc gagcggtatg ccgagatcgc caagcgcgcc tatgcgctgt ggg
//
LOCUS       DASYNS010000362         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2898834, whole genome shotgun sequence.
ACCESSION   DASYNS010000362 DASYNS010000000
VERSION     DASYNS010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2898834"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VGP50_11280"
     CDS             complement(<1..1199)
                     /locus_tag="VGP50_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEV8028006.1"
                     /translation="MPHILDYLADRVLLCDGAMGTQMQARTLDVERDYLGHENCTEIL
                     CESRPDLVREIHQGYLQAGADAVQTNSFGGSPITLGEFGIADKAFALNKRAGELAREA
                     VELFGGDGRARFVIGSVGPGTRLPSLGHADYQTIEDALTIQCRGLIAGGVDAILVETC
                     QDTLQIKAAVNGAKRARAEAAKDTPIFVQATVETTGTLLVGADIAAAATVIHALDVNM
                     IGLNCATGPLEMAEHVRWLGENWPGLISVQPNAGLPELMHGHTHYPLSAPELAQWQER
                     FVTEDGVNLVGGCCGTDIPHIAALDAMLRRIAPDGFRPLPKPRRSVWVPALASLYGQV
                     SLRQENAYLSIGERCNANGSKQFRQLQEKGEWDGCVEMAREQVKEGSHALDVCTAFVG
                     RDEIADMT"
     gene            complement(1225..2163)
                     /gene="metF"
                     /locus_tag="VGP50_11285"
     CDS             complement(1225..2163)
                     /gene="metF"
                     /locus_tag="VGP50_11285"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV8028007.1"
                     /translation="MSRAPALLGAEPLRPGVAPPLVSFEFFPPKTPELEATLWQTVKR
                     LEPLAPRFVSVTYGAGGSTRERTHATVLRIRNETKLEPAAHLTCVGSACDEIDTVARR
                     YWDAGIRHLVALRGDPPVGERYSPHRGGYAYAADLVAGLRRVADFELSVAAYPETHPE
                     APSPGRDLDNLKRKIDAGATRAITQCFFDVDVYLRFVDSAAAAGITVPIVPGILPATS
                     LAQARKFATTVPGWMAGLFEGLEEDPETRRLVAASITAEQCRRLQAEGVSEFHFYTLN
                     RADLIVAICHMIGVRGCASQPARAAGGDSLPTRATF"
     gene            complement(2160..>2433)
                     /locus_tag="VGP50_11290"
     CDS             complement(2160..>2433)
                     /locus_tag="VGP50_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEV8028008.1"
                     /translation="AEAARVLRPEGRLVIVDFAPHALEFLRSEHAHRRLGFADAEVTA
                     WCIAAGLEPEPARLLPGNPLTVALWQARRRARADVAAPAPALAEAS"
BASE COUNT          392 a          821 c          852 g          368 t
ORIGIN      
        1 gtcatgtcgg cgatttcgtc gcggccgacg aaggcggtgc agacatcgag ggcgtgcgag
       61 ccttccttca cctgctcgcg cgccatctcg acgcagccgt cccactcgcc tttctcctgg
      121 agctggcgga attgcttcga cccgttggcg ttgcagcgtt cgccgatcga gagataggcg
      181 ttctcctggc gcagcgatac ctgaccgtag agcgacgcga gcgccgggac ccagacgctg
      241 cgccgcggct tcggaagcgg ccggaacccg tccggggcaa tgcggcgcag catggcgtcg
      301 agcgccgcga tatgcggaat atcggtgccg cagcagccgc cgacgagatt gacgccgtcc
      361 tcggtgacga agcgctcctg ccattgggca agctcgggcg cgctcagggg ataatgcgtg
      421 tggccatgca tcagctcggg caacccggca ttgggctgga ccgagatgag gcccggccag
      481 ttctcgccga gccatcgcac atgctcggcc atctcgagcg gcccggtcgc gcaattgagg
      541 ccgatcatgt tcacgtcgag ggcatggatg acggtcgccg ccgccgcgat atcggcaccc
      601 acgagcaagg tgccggtggt ctcgaccgtc gcctgcacga agatcggcgt atccttggcc
      661 gcttccgcgc gggcgcgctt ggcgccgttc acggccgcct tgatctgcag cgtgtcctgg
      721 cacgtctcga cgaggatggc gtcgacgccg ccggcgatca ggccgcggca ctggatggtg
      781 agcgcgtcct cgatcgtctg gtagtcggca tggccgagcg agggaagccg tgtccccggc
      841 ccgaccgagc cgatgacgaa acgcgcgcgg ccgtcgccgc cgaacagctc caccgcctcg
      901 cgcgcgagct cgccggcgcg cttgttgagg gcgaaagcct tgtcggcgat gccgaactca
      961 cccaacgtta tgggcgagcc gccgaagctg ttggtctgca cggcatcggc gccggcttgc
     1021 agatatccct gatggatctc gcgcacgaga tcgggacgac tctcgcacag gatctcggtg
     1081 cagttctcgt gccccaggta atcgcgctcg acatcgagcg tgcgcgcctg catctgcgtt
     1141 cccatggcgc cgtcgcatag aaggacgcgg tcggcgagat aatcgaggat atgcggcatc
     1201 tgcgactctg aaatatcggg tgggttaaaa cgtggcgcgg gtcgggagcg agtctccgcc
     1261 ggcggcgcgc gcgggctgag acgcgcaacc gcgcacgccg atcatatggc agatggcgac
     1321 gatgagatcg gcacggttga gcgtatagaa atggaactcg ctcacgcctt ccgcctgaag
     1381 ccgccggcat tgctcggccg tgatcgacgc ggcgacgagg cggcgcgtct ccggatcttc
     1441 ctcgagcccc tcgaagaggc cggccatcca gccgggcacg gtggtcgcaa acttccttgc
     1501 ttgcgccaag ctggtggccg ggaggattcc cgggacgatc gggacggtga tcccggccgc
     1561 cgccgcgctg tcgacaaagc gcagatagac gtcgacatcg aaaaagcatt gcgtgatggc
     1621 gcgcgtggca cccgcatcga tcttgcgctt gaggttgtcg agatcgcgcc ccgggctcgg
     1681 cgcctcggga tgcgtctcgg ggtaggccgc aacgctgagc tcgaaatcgg cgacgcgcct
     1741 caacccggcg acaaggtccg ccgcgtaggc atagccgccg cgatggggcg aatagcgctc
     1801 gcccaccggc ggatcgccgc gcagcgcgac gagatggcgg atacccgcat cccaatagcg
     1861 gcgggccacg gtatcgatct cgtcgcaggc ggatccgacg caggtgagat gggccgcggg
     1921 ctcgagcttc gtctcgtttc ggatgcggag caccgtcgca tgggtccgct cgcgcgtcga
     1981 gccaccggcg ccatacgtca ccgacacgaa gcgcggcgcc aggggctcga gccgcttcac
     2041 ggtctgccag agcgtcgctt ccaactccgg cgtcttcggc gggaagaatt cgaaagagac
     2101 gagcggcggc gccacgcccg gacgcagcgg ctccgcaccg agaagtgccg gcgcgcggct
     2161 catgatgctt ccgccagggc cggcgccggc gcggcgacat cggcccgcgc gcgacgccgt
     2221 gcctgccaca gcgccaccgt cagcgggttc ccgggcagga gccgtgccgg ctcaggctcg
     2281 agaccggccg cgatgcacca cgcggtcacc tcggcatcgg cgaagccgag ccggcgatgg
     2341 gcatgctcgc tgcgcaggaa ctcgagcgca tgcggcgcga aatcgacgat caccagccgg
     2401 ccctccgggc ggagcacccg cgccgcctcg gcg
//
LOCUS       DASYNS010000363        19587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1806997, whole genome shotgun sequence.
ACCESSION   DASYNS010000363 DASYNS010000000
VERSION     DASYNS010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 19587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19587
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1806997"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VGP50_11295"
     CDS             complement(<1..207)
                     /locus_tag="VGP50_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028009.1"
                     /translation="MTTMLPGGDFAAVPELGYEEWRAVVRATAGRYSPDCVDPGAFAG
                     RVRARAIFGIGADRFDHNAHRIVRT"
     gene            442..1797
                     /locus_tag="VGP50_11300"
     CDS             442..1797
                     /locus_tag="VGP50_11300"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV8028010.1"
                     /translation="MITRGAFLKTRFAGYLHRDVPAEDAELAHVGPGTPCGEYLRRFW
                     QPVCFSDELRDVPERIKVLGEELVLFRDGSGAVGLLELHCPHRGASLEFGLVDCKGLR
                     CCYHGWLFATDGAILETPGEPAASTLKDRLYHGAYPVHEYGGIVFAYMGPPDRHPPFP
                     IYDSFVRPGYRLIPGQKYFYPCNWLQIMENAMDPVHTAFLHTIVSGAVFTDEFGILPE
                     LEFIETPVGMSYIATRRVGLNLWARMIDAILPNLQQVAPIWEDGQREHGFSGPMMSRW
                     IVPVDNTQTMLIEFRHVSESEGVAPRWWADRTIMLAGQLAADSYEAGQRHPGDYEAQV
                     SQRAVAIHGLEHLAATDRGVTMFRNLTRRGIRAVQAGGDPAGLSRDAGVVIPTYCNDT
                     VQRLPPDMDSAIDRQRLRQTGRRLAERYLEHPPLLAEAGTAPATNDVCMGPRERSPSM
                     T"
     gene            complement(1835..3664)
                     /locus_tag="VGP50_11305"
     CDS             complement(1835..3664)
                     /locus_tag="VGP50_11305"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEV8028011.1"
                     /translation="MNSKTIRIGGASGYWGDTASAPRQLVEKGDVHYLVFDYLAEVTM
                     SILAKQRQRSPDAGYATDFIALAMRPIISDIARKKIKIVANAGGVNLAACRTALEHLA
                     AEAGVALKIGTVEGDDLLPRAAALRAMDLRDMASGSPLPETLLSVNAYLGAFPIAAAL
                     AAGADIVVTGRCVDSALVLGPLIHEFGWAPDDYDRLAAGSLAGHLLECGAQVTGGNFT
                     DWQDVADGWDDMGFPVAAVSADGSFVVTKPEGTGGLVAPLTVAEQMLYEIGDPGAYVL
                     PDVVCDFTEVDMREDGRDRVRITGARGTPPTATYKVSATYPGGFGSTAAMVVAGFDTA
                     AKARRTGAAILAKTRRLLAAAGLGDFSLTAMQLIGAESLYGANARESAAASRELVLRL
                     AIRHAKKEGAELFARECVGSGLSMTTGRCGITPGRPDVSTAVLLHSSLIDKRKLDVTV
                     RVGGAAVPLPHAVTIGTDAPLPLARRREGEGTAPATLGRQIEVPLIRLAVARSGDKGN
                     DANIGVIARRPEYLPAIRAALTPEAVKRYFAHFVEGEVERFDLPGIHALNFVLHASLG
                     GGGTASLRLDTQAKTYAQLLLGMAVPVPEAWSNALGVASSHDG"
     gene            complement(3768..4913)
                     /locus_tag="VGP50_11310"
     CDS             complement(3768..4913)
                     /locus_tag="VGP50_11310"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8028012.1"
                     /translation="MTPAGSPYYTAEHEAFRETLRRFVAKEIEPYAAAWDEAETFPRE
                     LYVKAAGIGLTALGFPEEFGGVQTDSFYGIIAAQELARAGAGGIAASLQSHTIGAPPI
                     AFAGSAELKRRVLPEILSGRKISALAITEPGAGSDVASLETAARREGEHYVVKGSKTF
                     ITSGMRADYYTVAVRTGGPGRGGISLLVIERDTPGFARTPLKKMGWWASDTATLYFDD
                     CRVPAGNLLGEENQGFALIMRNFNRERLGMAAACIGYARVCFDDAVTYSRERRTFGQR
                     LVEHQAIRHKLVDMAMRVNAAQAYLESTAWRVDRGENPVADICMLKNQATLTMEFCAR
                     EAVQIFGGAGFMRGPKVERIYREVRVNAIGGGAEEIMRDLAARQLGF"
     gene            complement(4919..6880)
                     /locus_tag="VGP50_11315"
     CDS             complement(4919..6880)
                     /locus_tag="VGP50_11315"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV8028013.1"
                     /translation="MTAFAKILIANRGEIACRVARTARAMGYRTVAVYSDADRHAPHV
                     AAADQAVPIGPPQAAQSYLAIDKIIAAARRSGADAIHPGYGFLSENADFAAACEAADI
                     TFIGPSPDAIRAMGHKAAARRQMSEIGIRCVPGYDGAEQSEHVLARAAAQIGFPVMVK
                     AAAGGGGKGIRLVATPDRLAGALTAARSEAAKAFGSDELILEKAIAAPRHVEIQIFAD
                     RHGNVIHLFERECSIQRRHQKVIEEAPSPAMTPALRSAMGEAAVAVARAIGYVGAGTV
                     EFLLTPDGHFYFLEMNTRLQVEHPVTEMITGLDLVEWQLHVAAGEALPLRQDAVTLGG
                     HAIEARLYAEDAHADFLPQAGTVLAWEPPSGAGLRVDHGIAPGLTVSPYYDPLLAKIV
                     AHGATRQEARRRLASALQDTLVLGIETNRRFLIDCLAHPAFVAGDTSTAFIERHLPPA
                     SRRRPAADARMIALAAALIAGTAKPGLGHWHSSGKASSPMLLRSGDAKTAIDFVEDEA
                     GSCRVAWNGTSQALEILAVDGTSVRFLADGLVETARFAWQEGALLLSLDGVTERFEDV
                     LLAPRDGGAAAGAAAALAPMTGMIAAVRVKAGDAVRKGQCLVILEAMKMEHEILAPRD
                     GTVSSVLVTPGEQVKTRKLLVELAPLAAE"
     gene            complement(6877..7689)
                     /locus_tag="VGP50_11320"
     CDS             complement(6877..7689)
                     /locus_tag="VGP50_11320"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV8028014.1"
                     /translation="MLPSCKTLLLAQDGPRLAITLNRPDTRNALDSEMVGELTAVVDC
                     LESARDIGCVVLRGAGGTFCAGGDVRGFMAQFASAPPPPGERDPIALNNRRFGAFLAR
                     FDNLPQTIVVAVEGAAFGGGLGLAAIGDVVIAMADARFAMSETGLGVIPAQIAPFVAV
                     RVGVSAARRLALTGLRFDGREAARIGLVHQVADDKAGFAAALAKTLADIDRCAPGANA
                     ATKRLLLSSRTTPLDALLDDAADAFAAALRGAEAKEGVAAFLEKRPAAWSRR"
     gene            complement(7784..8956)
                     /locus_tag="VGP50_11325"
     CDS             complement(7784..8956)
                     /locus_tag="VGP50_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8028015.1"
                     /translation="MIYTEQHHELMRSVAKFCEAEINPHVEEWEKAGIFPAHELFKKM
                     GNQGFLGINKPEKFAGLGLDYSYAVAFCEALGASRSASVKMAIGVQTDMATPALARHG
                     SDALREEFLRPSITGDYVACLGVSEVGAGSDVASIKTRARKDGGDYVIDGGKMWTTNG
                     TQADWICLLANTGDGPVHRNKSLICVPMKTKGVEIARKLDKLGMRASDTAQIFFDGVR
                     VPQRHRIGEEGMGFIYQMEQFQEERLWAAAGALVGMEHTIQDTIDYTRNRVAFGQSIL
                     DNQVVHFRLAELQTKIELLRSLVYRAVEDYLAGADVTNLASMAKLTVGRLQREVADSC
                     LQYWGGMGYMSESAISRVFRDSRLGSIGGGADEVMLGIIAKHMGTLPGKKRPGANE"
     gene            complement(8964..10583)
                     /locus_tag="VGP50_11330"
     CDS             complement(8964..10583)
                     /locus_tag="VGP50_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV8028016.1"
                     /translation="MPVIESRIDPDGESFRANRRAMLGLVEEFRALEDRVRRTSNAKK
                     EQFAQRGQLLPRERVALLLDRGSSFLEISTLAGLGMHDDDGKENPLGAGGIDGIGYVS
                     GVRCLIAASDSAIKGGTTAPMGLKKRLRCQEISRENKLPMINLVESGGANLNYQAEIF
                     VEGGQTFRNQARLSAMGVPQVTIVHGSSTAGGAYLPGLSDYVVMIKGQAKVFLAGPPL
                     LRAATGERASDEELGGAEMHSTISGVSDYMAEDDADGIRLCREIMAKLRWNDRLPPEP
                     RRSFREPRYSPDELCGVVPVDYRKPYDVHEVVARIVDGSDFLDFKPLYGGHTICGQGE
                     IEGLPVGIIANNGPIDADGSVKAAQFIQLMCQSGTPIVYLQNTTGYMVGTEAERSGIV
                     KHGSKMIQAVSNASVPQITLHVGASFGAGNYGMCGRAYGPRFIFAWPNNRIAVMGGEQ
                     AAKVLSIITEDRMARAGKPIDRAQLQAMEQKIVEQFDRESTALFATARLWDDGLIDPR
                     DSRKVLAFCLSICREAEIRPLRATSFGVARM"
     gene            complement(10583..11464)
                     /locus_tag="VGP50_11335"
     CDS             complement(10583..11464)
                     /locus_tag="VGP50_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8028017.1"
                     /translation="MSYRSVFRGGLFAGRHVVVTGGGSGIGRCTAHELAALGAAVTLI
                     GRKAARLAAVAAEIADAGCPRPGVHVCDIRSEEEVERTVAAVLATYSPIDGLVNNAGG
                     QFSAPLKDISQKGWETVVRTNLTGGFLMARECFRRSMAEHGGAIVNIIADMWASMPGM
                     GHSGAARAGMLNFTQTAALEWASSGVRVNAVAPGWIASSGLATYPPEMRKELKELWRH
                     VPLKRLGTESEVSAAIVFLLSEAASFISGACLRVDGAAPNARTNWPMPDHDRSAAYYG
                     FPLESPPGFLRREKGRV"
     gene            11568..12194
                     /locus_tag="VGP50_11340"
     CDS             11568..12194
                     /locus_tag="VGP50_11340"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8028018.1"
                     /translation="MAVTAMKEGVSRFKRALILGAAARLFSERGYAATTIDAITEELK
                     ASRRAIYDHFVGKSDILAAICEQAVRFAAELAERVAREPGDPAAKLSRLVRDFTLIVI
                     ENQDYIAISSREMKYLPEEAQRRIFHLQERFDRILSAMLQEGVRAGTLDTADPEMTGL
                     AISGMIIWVHGWYRPRGRLSPAEIAERMAETALRMAHARKGGQPAAAR"
     gene            12274..13938
                     /locus_tag="VGP50_11345"
     CDS             12274..13938
                     /locus_tag="VGP50_11345"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV8028019.1"
                     /translation="MLEGLQMNLPLAVSCLIDHAAAYHADTEIVARTIEGDVHRTTYG
                     AVGSRTKRLARALLRLGVAAGDRIGSLAWNTHRHFEMFYGVSGTGAVLHTINPRLFAE
                     QLVYIINHAEDRILFIDAATLPVFEQIAPLLTTVRQCVMMAPRERMPAKSAAGDFLCY
                     EELLAAEDDDYQWPEFDERAASTICYTSGTTGNPKGVVYSHRSAMLTTMLINSMVGLG
                     SRNGAREVLMPMAPMFHGNAWQFPYVAPMVGAKLVLPGRNYEPQKLYELIEEEGVTIT
                     CGVPTLWIMLADWLDRTNQRFSKLRLTLSSGSAPPRALVEKLESNYGIEFVQAWGMTE
                     ALGGSNALMEPGSADLPFEERIDRRMNSGRTLWGVKYRIVDDEERVLPHDGKASGHLR
                     VKGPWIASGYFKGEGGSALDRDGWLKTGDVASIDPRGHILLTDRSKDVIKSGGEWISS
                     IALEGVAAGHPEVLQAAVIGVTHPKWQERPLLVVVRKQGSTLSGGALLDFMRDKVATW
                     WLPDDVAFIDAMPMTGTGKVHKLTLRTQFRDYVLPDLERVGRASHG"
     gene            complement(14281..15174)
                     /locus_tag="VGP50_11350"
     CDS             complement(14281..15174)
                     /locus_tag="VGP50_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV8028020.1"
                     /translation="MDWDKLRVFHAVAEAGSFTHAGEALNLSQSAVSRQISALEESLS
                     VPLFHRHARGLILTEQGELLYHTAREIFSKLAMTEAMLSESKDRPKGPLKVTTTVSFG
                     SAWLTPRIREFTDLYPEIQVTLVVDDSELDLSMREADVAIRMSPPRQPDLVQRHLMTV
                     RYRVYASTDYLKRFGTPQKPEDLDKHRLIVYGEDSRQPVRNINWLREVGARAGIDRQP
                     IFMVNNVYAIFRAVESGLGIAALPDFMGQEAASLVRILPELAGPEVEAYFVYPEELRS
                     SKRIAVFRDFLLRKVTEGRNS"
     gene            complement(15208..16179)
                     /gene="trxB"
                     /locus_tag="VGP50_11355"
     CDS             complement(15208..16179)
                     /gene="trxB"
                     /locus_tag="VGP50_11355"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV8028021.1"
                     /translation="MTQRHHTKFLIIGSGPAGFTAAIYAARANLQPILVQGLQPGGQL
                     TITTDVENYPGFGEVIQGPWLMEQMAKQAAHVGTRLMSDLIVSVDLSKRPFVCVGDSG
                     DIYVSDTLALCTGAQARWLGIPSETKFRGAGVSACATCDGFFFRDREVVVVGGGNTAV
                     EEALYLTNHATRVTLIHRRDRLRAEKILQARLFANPKIHIVWDSAVEEILGEGMPPRV
                     TGVRIANVRTGARSVIPCDGFFVAIGHTPVTDIVRGQLALDRDGYILTKPDSTVTDVP
                     GVFAAGDVKDKVFRQAVTAAGMGCMAALEAEKFLAVEEAAAALKAAE"
     gene            complement(16204..18108)
                     /locus_tag="VGP50_11360"
     CDS             complement(16204..18108)
                     /locus_tag="VGP50_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028022.1"
                     /translation="MPLRIETFNNNRGGNAFFKAVTHPLAARAMERLLCRLGGSPLAV
                     YDPDGLADGLSDLYPLSRLDIAGCFVQDVTAIGRQILGQRAQPVTELRESPARRMLVA
                     AFDAGRAVGHIRHLLPPGAEVATLDEIRLPDELLTNRRRYLDPVNFATNFVFFRDQAG
                     LHTRLVTANYWAGYGASSLTLWLLLFDAEGKVLAEWSEPVAAGTASIILDSRAIRARF
                     ALPEFTGQLFLHAIGAVGQDVVKYALDTFGDDAASCFSCTHDANSWPADYYAGLPAPD
                     SGEAVTLWVQNSQPCAIPAGTVGLNLMGDAALAAIDREIAPFASHAVDIASMLPAARW
                     PQQIEVSAGKYVVRPRYEVVRGGRRRIAHVNVERTDLKPDPRLPELGNMLGKGYLLPA
                     PILPRGTWRSLALPTPMARTQEDLPIALVVYDGAGRESARHRFGRLLRRDSVAVDLDG
                     VIDGAALPSGYGHLELVYDFRDGGSGDGWLHGLFRYEDRASGHMAETSFGAHVFNTVL
                     TYKDEPQSYAGRAPGLSTRLFLRLGERPHDTFCHLIYPASMPWHDESATDLVLHDGSG
                     RELARRQVAIPCSGSLFWRYGEMFDQGLRDRAGDGAYIIIRDTTCRLFGYHGLLAKGG
                     AFSLDHMFGF"
     gene            18363..>19587
                     /locus_tag="VGP50_11365"
     CDS             18363..>19587
                     /locus_tag="VGP50_11365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV8028023.1"
                     /translation="MFALTLARLAWLSLGRADLYPDEAQYWLWSLHPALGYYSKPPLV
                     AWLIAATTALFGEDEFAIRLSAPLLHFATSLVIYMLARRLYDARVALWSAVAYATLPG
                     VSASAALISTDAPLLLCWGVALYAFVRAREPGGGRWWLAVGVAAGIGLLAKYAMVFWL
                     MSALLFLLLFRDERRHASRFLGATALALAIYAPNFLWNAANGFVSYRHTSDNAAFGGQ
                     LLHPLHFLEFFGSQFAVFGPIFFAVLIFLALTARRTLVERRAALLAAFALPTLLIMLA
                     ESFLSRAQPNWSAPTFISAVVLVVAWLVIEGRQTWIVASVALHATAAVVCFAAHDVSA
                     ALGAPLPAKYDLLHRLRGWKVLGRSVSQSLASHPGTLLMSDDREDMAALIYYVRPHPF
                     DALKWNGEGGIHDGFD"
BASE COUNT         3233 a         6377 c         6772 g         3205 t
ORIGIN      
        1 cgtcctgacg atgcggtgag cattgtgatc aaagcggtcg gcgccgattc caaatatggc
       61 gcgcgcgcgc accctgccgg cgaaggcacc ggggtcgacg cagtcggggc tgtaccgccc
      121 ggcggtcgcg cgaacgacgg ctcgccattc ctcgtacccc aattccggca cggccgcgaa
      181 atcgccgccc ggaagcatcg tcgtcacgtc gctacctcgt acaaatcgcc aatcagttga
      241 atctgcgggg agtggatgcg accagcgtgg gcatccaaac cgactccgca gctcagcgtc
      301 gcaatatcag atgtggtgcg cggatcgggt tttcccagcg cgctcctggt gcgcgccacg
      361 actactcatg gtgcgcgttc gtctcacgac gtaggcgggt cgatgaacta attgtgtggg
      421 gaatatgcgg ggagccctgc catgatcacc agaggcgctt tcttgaagac ccgatttgcc
      481 ggttaccttc atcgtgatgt gcccgccgag gacgcggaac tcgcgcatgt cggccctggc
      541 acgccatgcg gcgaatatct gcggcgcttc tggcagccgg tctgcttctc ggacgagctg
      601 cgcgatgtcc cggaacggat caaggtcttg ggcgaggagc tggtgctgtt ccgcgatggg
      661 agcggcgccg tcgggctctt ggagttgcat tgtccccatc gcggcgcgtc cttggaattc
      721 gggctcgtcg actgcaaggg cttgagatgc tgctaccatg gctggctgtt cgccacggac
      781 ggcgccatcc tcgagacgcc gggcgagcct gcggccagca ccttgaaaga ccgcctctat
      841 cacggcgctt atcccgtcca tgagtatggc ggcatcgtct tcgcctatat gggtccgccc
      901 gatcggcacc cgccttttcc gatctacgac agctttgtcc ggccgggata tcgtctcatt
      961 ccaggccaga agtacttcta tccatgcaac tggctgcaga tcatggagaa cgccatggat
     1021 cccgtccata cggcgttcct ccacaccatc gtcagcggcg ccgtgttcac cgacgagttc
     1081 gggatactcc cggagctcga gttcatcgag acgcccgtgg ggatgagcta cattgcgacg
     1141 cgacgggtgg ggctcaatct gtgggcgcgg atgatcgacg ccatcctacc caacctgcag
     1201 caggtggcgc cgatctggga ggatggccag cgcgagcatg ggttcagcgg tccgatgatg
     1261 agccgttgga tcgtgccggt ggacaacacc caaaccatgc tcatcgaatt ccgccatgtg
     1321 agcgagagcg aaggcgtcgc gccccgatgg tgggccgatc gcaccatcat gctcgccggc
     1381 cagctcgccg ccgactccta tgaggccggc cagcgtcatc ccggcgacta tgaggcgcaa
     1441 gtctcgcagc gcgccgtcgc catccacgga ttggagcatc tcgccgcgac cgatcgtggc
     1501 gtgacgatgt tccgcaacct gacgcggcgc gggatacggg ccgtgcaagc cggtggcgat
     1561 cccgctggcc tcagccgcga tgccggcgtg gtcattccga cctattgcaa tgacactgtc
     1621 cagcgcttgc cgcccgatat ggattcggca atcgaccggc agcgcctgcg ccaaaccgga
     1681 cggcgcctcg cggagcgata tctcgagcac ccgccgctcc tggcagaagc cggcaccgcg
     1741 ccggcgacca atgacgtatg catgggtccg cgcgagcggt ccccgagcat gacatgacga
     1801 gctttgcgaa ggctgccccg acataagcgc gaggtcagcc gtcgtgcgac gatgcgacgc
     1861 cgagcgcgtt gctccacgct tccgggaccg gcacggccat gccgagcaga agctgcgcgt
     1921 aggtcttggc ttgggtgtcg aggcgcagcg acgcggtgcc gccgccaccc aggctcgcat
     1981 gcagcacgaa gttgagcgcg tggatgccgg ggagatcgaa gcgctcgacc tcgccttcga
     2041 cgaaatgcgc gaagtagcgc ttcaccgctt cgggcgtcag cgcggcgcgg atcgccggga
     2101 gatattccgg gcgccgggcg atcacgccga tattggcgtc gttgcctttg tcgccgctgc
     2161 gcgcgacggc gaggcggatg agcggcacct cgatttgccg gccgagagtc gccggcgccg
     2221 ttccctcacc ctcccgtcgc cgcgcgaggg gtaagggagc gtcagtgccg atggtcacgg
     2281 catggggcaa aggcactgcg gccccgccga cgcgcaccgt gacgtcgagc ttgcgcttgt
     2341 cgatgagcga cgaatggagc aggacggcgg tggacacatc gggccggccg ggcgtgatgc
     2401 cgcaacggcc cgtcgtcatg ctgaggcccg agccgacaca ttcccgggcg aaaagctcgg
     2461 cgccttcttt tttcgcgtga cggatggcga ggcgcagcac gagctcgcgg ctcgcggcgg
     2521 cactctcacg cgcattggca ccatagagcg actcggcgcc gatgagctgc atcgccgtga
     2581 ggctgaaatc gccaagccca gccgctgcca gcaggcgccg cgtcttggcg aggatggccg
     2641 cgccggtgcg ccgcgctttc gctgccgtgt cgaagccggc caccaccatc gcggcggtcg
     2701 agccgaagcc gccggggtat gtggcactca ccttgtaggt ggccgtcggc ggcgtgccgc
     2761 gggcgccggt gatccggacg cgatcgcgcc cgtcttctct catgtcgacc tctgtgaaat
     2821 cgcagacgac gtcgggcagc acataagcgc cgggatcgcc gatctcgtag agcatctgct
     2881 cggccaccgt gagcggcgcg acgaggccgc cggtgccctc gggcttggtg acgacgaagc
     2941 tgccatcggc cgacaccgcg gcgacgggaa agcccatgtc gtcccagcca tcggccacgt
     3001 cctgccaatc ggtgaagttg ccgccggtga cctgcgcgcc gcattcgagg aggtggccgg
     3061 cgaggctgcc ggcggcgagg cggtcgtagt catcgggcgc ccagccgaac tcatggatga
     3121 gcgggcccag gaccagcgcg ctgtcgacgc agcgtccggt gacgacgatg tcggcgccgg
     3181 cggcaagcgc cgcggcaatg ggaaaggcgc cgagataggc attgacgctg agcaaggtct
     3241 ccggcaacgg ggagccactc gccatgtcgc gcaggtccat ggcacgcagc gccgcggcgc
     3301 gcggcagaag gtcgtcgcct tcgacggtgc cgatcttgag cgcgacaccg gcttcggcgg
     3361 cgaggtgctc gagcgccgtg cggcaggcgg cgagattgac gccgccggca ttggcgacga
     3421 ttttgatctt cttgcgcgcg atatcggaga tgatcggcct catcgccagc gcgatgaaat
     3481 cggtggcgta gccggcgtca ggcgagcgct ggcgctgctt ggcgaggatc gacatcgtca
     3541 cttcggcgag atagtcgaag acgaggtagt ggacatcgcc cttctcgacg agctggcgcg
     3601 gcgcggacgc agtgtcgccc caatagccgc tggccccgcc gatgcggatg gttttcgagt
     3661 tcatcgcgag cccacccctc accctcccac tgctgcgcag tgggcccctc cctctcccac
     3721 agaggggaga ggggatgatg tcgccgacgg aaggatgcag ctcatgacta aaaccccagc
     3781 tgccgtgcgg cgaggtcgcg catgatttct tcggcgccgc cgccgatggc gttgacgcgg
     3841 acctcgcggt agatgcgctc gaccttgggg ccgcgcatga agccggcgcc gccgaagatc
     3901 tgcaccgctt cgcgcgcgca gaactccatg gtgagcgtcg cctggttctt gagcatgcag
     3961 atgtcggcca ccgggttctc gccccgatcg acgcgccagg cggtgctctc gagataggct
     4021 tgcgcagcgt tgacgcgcat tgccatgtcg accagcttgt ggcggatcgc ctgatgctcg
     4081 acgaggcgct ggccgaaggt gcggcgctcc cgcgaatagg taacggcgtc gtcgaagcag
     4141 acgcgggcgt agccgatgca ggctgcggcc atgccgaggc gctcgcgatt gaagttgcgc
     4201 atgatcagag cgaagccctg attctcctcg cccagcagat tgccggcggg cacgcggcag
     4261 tcgtcgaaat agagcgtcgc cgtgtcggag gcccaccagc ccatcttctt gagcggcgtg
     4321 cgcgcgaagc ccggggtatc gcgctcgatc accaggagcg agatgccgcc gcggccagga
     4381 ccgccggtgc ggacggcgac ggtgtagtag tcggcgcgca tgcccgaggt gatgaaggtc
     4441 ttcgatccct tgacgacgta gtgctcgccc tcgcgccgcg ccgcggtctc gaggctggcg
     4501 acgtccgagc cggcgccggg ctcggtgatg gcgagcgcgc tgatcttgcg gccgctcaga
     4561 atctccggca ggacgcggcg cttcaattcg gcgcttccgg caaaggcgat gggcggcgcc
     4621 ccgatggtgt ggctctggag actggcggca atgccgccgg cgccggcgcg cgccagctcc
     4681 tgcgcggcga tgatgccgta gaagctgtcg gtctggacac cgccaaactc ctcggggaag
     4741 cccagcgccg tcaggccgat gccggcggct ttgacataga gctcgcgcgg gaaagtctcg
     4801 gcctcgtccc aggctgcggc atagggctcg atctccttgg cgacgaagcg gcgcagagtc
     4861 tcgcggaagg cctcatgctc cgcggtgtag taagggctgc cggccggcgt catcgtcgtc
     4921 actcggcggc caagggcgcc agctcgacca ggagcttgcg cgtcttgacc tgttccccgg
     4981 gcgtcaccag caccgacgac accgtgccgt cgcgcggcgc caggatctca tgctccatct
     5041 tcatggcttc gaggatcacg aggcattggc ccttgcgcac ggcatcgccg gccttgacgc
     5101 gcacggcggc gatcatgccg gtcatcggcg cgagcgccgc agcggctccc gcggcagcgc
     5161 cgccgtcgcg cggcgccagc agcacgtcct cgaagcgctc cgtgacgccg tcgagagaga
     5221 ggagcaaggc gccctcctgc caggcgaagc gcgccgtctc gacgagccca tccgcgagga
     5281 agcgcacgct cgtgccatcg acggcgagga tctcgagcgc ctggctcgtg ccattccagg
     5341 cgacgcggca gcttccggct tcgtcttcga caaagtcgat ggcggtcttc gcgtcgccgc
     5401 tcctaagcag catgggggag gacgccttgc cgctgctgtg ccaatggccg aggccgggct
     5461 tcgccgtgcc ggcgatgagc gcggcagcga gcgcgatcat ccgcgcatcg gccgccggcc
     5521 gacggcgcga agctggcggg aggtggcgtt cgatgaaagc ggtgctggtg tcgccggcaa
     5581 cgaaggccgg gtgggcgagg cagtcgatga ggaagcggcg gttcgtctcg atgccgagca
     5641 ccagcgtgtc ctgaagggcc gaggcgagcc ggcgccgcgc ctcttggcgt gtcgcgccat
     5701 gagcgacgat cttcgccagg agcggatcgt agtagggcga gacggtgagg cccggagcga
     5761 tgccgtgatc gacgcgcaaa cccgcgcccg agggcggctc ccaggcgagc acggtcccgg
     5821 cctggggcag gaaatcggca tgggcgtcct cggcatagag ccgcgcctcg atggcgtggc
     5881 cgccgagcgt cacggcgtcc tggcgcaacg gcagcgcctc cccggcagcg acgtggagct
     5941 gccattcgac gagatcgagg ccggtgatca tctcggtcac gggatgctcg acctggagcc
     6001 gcgtgttcat ctcgaggaaa tagaagtggc cgtcgggcgt cagcaggaac tcgacggtgc
     6061 cggcgccgac atagccgatc gcccgggcga cggcgacagc ggcctcgccc atggcgctgc
     6121 gcagcgccgg cgtcatggcg ggcgacggcg cttcctcgat gaccttctga tggcggcgct
     6181 ggatcgagca ctcgcgctcg aagagatgga tcacgttgcc atggcggtcg gcgaagatct
     6241 ggatctcgac atggcgcggc gcggcgatgg ccttctcgag gatgagctcg tccgaaccga
     6301 acgccttcgc cgcttcggag cgcgcggcgg tcagcgcgcc ggcgaggcgg tccggtgtcg
     6361 cgacgaggcg gatgcctttg ccgccgccgc cggccgcggc cttcaccatc actggaaaac
     6421 cgatctgcgc ggcggcgcgg gccagcacgt gctcgctctg ctcggcgccg tcatagccgg
     6481 ggacgcagcg gatgccgatc tcgctcatct gtcgccgggc cgcggccttg tggcccatgg
     6541 cgcggatcgc gtcgggcgag gggccgatga aggtgatgtc ggcagcttca caggccgcgg
     6601 cgaaatcggc attctcggaa agaaagccgt agcccggatg gatggcgtcg gcgccggagc
     6661 ggcgcgccgc ggcgatgatc ttgtcgatcg cgagatagga ttgcgccgct tgcggcgggc
     6721 cgatcggcac ggcctggtcg gccgcggcca catgcggcgc gtgacgatcg gcatcggaat
     6781 agacggcgac ggtgcgatag cccatggcgc gcgccgtgcg ggcgacgcgg caggcgatct
     6841 cgccgcgatt ggcgatgagg atcttcgcga acgccgtcac cggcggctcc aggcggcagg
     6901 gcgcttctcg agaaaagccg cgacgccttc cttggcctcg gcgccacgca aggccgcggc
     6961 aaaggcatcg gcggcgtcgt cgagcagcgc gtcgagcggc gtggtgcggc tcgacagcag
     7021 caaccgcttg gtggcggcgt tggcaccggg ggcacagcgg tcgatgtcgg cgagagtctt
     7081 cgcgagcgcc gccgcgaagc cggctttgtc gtccgcgacc tgatgcacca ggccgatgcg
     7141 cgcggcctcg cggccgtcga atcgcaagcc ggtgagcgcg aggcgccgcg cggcggagac
     7201 gccgacgcgc acggcgacga agggcgcgat ctgcgccggg atgacgccga ggccggtctc
     7261 cgacatggca aagcgcgcat ccgccatggc gatgacgaca tcgccgatcg ccgcaaggcc
     7321 gagcccgccg ccgaaggccg cgccttcgac ggcaacgacg atggtctgcg gcaggttgtc
     7381 gaaacgcgcg aggaaggcgc cgaagcggcg gttgttgagc gcgatcgggt cacgctcgcc
     7441 gggcgggggc ggcgcgctcg cgaattgcgc catgaagccg cgcacgtcgc cgccggcaca
     7501 aaaggttccg ccggcgccgc gcaggacgac gcagccgatg tcgcgggcgg attcgaggca
     7561 gtcgacgacg gcggtgagct cgccgaccat ctcgctgtca agagcgttgc gcgtgtcggg
     7621 acggttcagg gtgatggcga gacgcgggcc atcctgtgcc agaagcaagg tcttgcagct
     7681 tgggagcatc gtcaccgacc ccccacccct ccctccccct caagagggga gggggaatgc
     7741 gtttcggagg ggtggggagt atggaacgcg cgcgacatcg cggtcactcg ttcgcgcccg
     7801 ggcgcttttt gccggggagg gtgcccatgt gcttcgcgat gatgccgagc atgacctcgt
     7861 cggcgccgcc gccgatcgag cccaggcggc tgtcgcggaa gacgcggctg atcgcgctct
     7921 cgctcatgta gcccatgccg ccccaatatt ggaggcagct gtcggcgacc tcgcgctgga
     7981 gacggccgac ggtgagcttc gccatggagg cgagattggt gacgtcggcg ccagcgagat
     8041 agtcctcgac ggcgcgatag accagcgagc gcagcagctc gatcttggtc tgcagctcgg
     8101 ccaggcggaa atgcacgacc tggttgtcga gaatgctctg gccgaaggcg acgcggttgc
     8161 gcgtataatc gatggtgtcc tggatcgtgt gctccatgcc gacgagcgcg ccggcagcag
     8221 cccagagccg ctcctcctgg aattgctcca tctgatagat gaagcccatg ccttcctcgc
     8281 cgatgcggtg gcgctgcggc acgcggacgc cgtcgaaaaa gatttgcgcc gtgtcggaag
     8341 cgcgcatgcc gagcttgtcg agcttgcggg cgatctcgac gcccttggtt ttcatcggca
     8401 cgcagatcag cgacttgttg cgatggacgg ggccgtcgcc ggtattggcg aggaggcaga
     8461 tccaatcggc ctgcgtgccg ttggtggtcc acatcttgcc gccgtcgatg acatagtcgc
     8521 cgccgtcctt gcgggcgcgc gtcttgatgc tggcgacatc ggagccggcg ccgacttcgg
     8581 acacgccgag acacgcgaca tagtcgccgg tgatgctggg ccgcaggaat tcctcgcgca
     8641 gcgcgtccga gccatgccgc gccagcgccg gcgtcgccat gtccgtctga acgccgatcg
     8701 ccatcttgac cgaagcgctg cgcgacgcgc ccaaggcctc gcaaaaggcc accgcgtagg
     8761 aatagtcgag gccgaggcca gcgaatttct ccggcttgtt gatgccgagg aagccttggt
     8821 tccccatctt tttgaaaagc tcgtgcgccg ggaagattcc ggccttctcc cattcctcga
     8881 catgagggtt gatctcggcc tcgcagaatt tcgcgaccga gcgcatgagc tcgtggtgct
     8941 gttccgtgta gatcatgaga gcctcacatg cgggcgacgc cgaagctggt ggcgcggagc
     9001 gggcggatct ccgcttcgcg gcagatcgag agacaaaagg cgaggacctt gcggctgtcg
     9061 cgcgggtcga tgagtccatc gtcccaaagc cgtgccgtgg cgaaaagcgc cgtcgattcg
     9121 cggtcgaact gttcgacgat cttctgctcc atcgcctgga gctgcgcccg gtcgatcggc
     9181 ttgcccgccc gcgccatgcg gtcctccgtg atgatcgaga gcactttcgc ggcctgctcg
     9241 ccacccatga cggcgatgcg gttattgggc caggcgaaga tgaagcgcgg accataggcg
     9301 cggccgcaca tgccgtaatt gcccgcgccg aagcttgcgc cgacgtgcag ggtgatctgc
     9361 ggcacgctcg cgttgctcac cgcctggatc atcttcgagc cgtgcttgac gatgccgctg
     9421 cgctcggcct ccgtgccgac catgtagccc gtcgtgttct ggagatagac gatcggcgtt
     9481 ccggactggc acatgagctg aatgaactgc gccgccttga ccgagccatc ggcgtcgatc
     9541 gggccgttgt tggcgatgat gccgacggga aggccctcga tctcgccttg gccgcagatg
     9601 gtgtggccgc cgtagagcgg cttgaagtcg aggaaatcgc tgccgtcgac gatgcgcgcg
     9661 acgacctcgt gcacgtcata gggcttgcgg tagtcgacgg gcacgacacc gcagagctcg
     9721 tcggggctgt agcgcggctc gcggaagctg cgccggggct ccggcggaag acgatcgttc
     9781 cagcggagct tcgccatgat ctcgcggcag aggcggatgc cgtcggcatc gtcctcggcc
     9841 atgtagtcgc tgacgccgga gatcgtcgaa tgcatctcgg cgccgccgag ctcctcgtcg
     9901 ctggcgcgct cgccggtggc ggctcggagc agcggcgggc cggcaaggaa caccttcgcc
     9961 tgccccttga tcatgacgac gtagtcggaa aggcccggca gataggcgcc gcccgcggtc
    10021 gacgagccat ggacgatggt cacctgcggc acgcccatgg ccgaaagccg cgcctggttg
    10081 cggaaggtct gcccgccctc gacgaagatt tccgcctggt agttgagatt ggcgccgccg
    10141 gactcgacga ggttgatcat cggcagtttg ttctcgcgcg agatctcctg gcagcgcagc
    10201 cttttcttga gccccatggg cgcggtcgtg ccgcccttga tggcgctgtc ggaggcagcg
    10261 atcaggcagc gcacgcccga gacatagccg atgccgtcga tgccgccggc gcccagcggg
    10321 ttctccttgc cgtcatcgtc atgcatgccg aggccggcga gcgtcgagat ctcgaggaaa
    10381 gagctgccgc gatcgagcag cagcgccacg cgctcgcgcg gcaagagctg gccgcgctgc
    10441 gcgaactgct ccttcttcgc attggaggtg cggcgcacgc ggtcctcgag cgcgcggaac
    10501 tcctcgacga gaccgagcat cgccctgcgg ttggcgcgga agctctcgcc gtcgggatcg
    10561 atgcggctct cgatgacggg catcaaaccc tgcccttctc acgccggagg aacccgggcg
    10621 gcgattcgag agggaagccg taatacgccg ccgagcggtc gtggtccggc atcggccagt
    10681 tggtccgcgc gttgggcgcg gcgccgtcga cgcgcaggca agcgccggaa atgaaggacg
    10741 cggcctcgga gagcagaaag acgatcgccg ccgacacctc gctttcggtg ccgagccgct
    10801 tcagcggcac atggcgccag agctccttga gctctttccg catctccggc gggtatgtcg
    10861 cgaggcccga ggacgcgatc cagccgggcg ccaccgcgtt gacgcggacg ccggacgagg
    10921 cccattcgag cgccgcggtc tgcgtgaaat tgagcatgcc ggcgcgcgcc gcgccggaat
    10981 gccccatgcc gggcatcgac gcccacatat cggcgatgat gttgacgatg gcgccgccat
    11041 gctctgccat cgagcgccgg aagcattcgc gcgccatgag gaagccaccg gtgaggttgg
    11101 tgcgcaccac cgtctcccag cccttctggg agatgtcctt gagcggcgcc gagaattgcc
    11161 cgccggcatt gttgacgagc ccgtcgatcg gggaatacgt cgcaaggacg gccgcgaccg
    11221 tccgctccac ctcttcttcg ctgcggatgt cgcagacatg gacgccgggg cggggacagc
    11281 cggcatcggc gatctcggcg gcgacggcgg cgaggcgcgc cgccttgcgt ccgatgagcg
    11341 tcactgctgc gcccagcgcc gccagctcat gggcggtgca acggccgatg ccgctgccgc
    11401 cgccggtcac gacgacatgg cgtccggcga agagcccgcc gcggaacacc gagcgatagc
    11461 tcatggcgcc tcccagcgct ttggtacttg tcgttctcga aaaggtactg gacagtaccg
    11521 gccgcgtcaa cgcaggatga gagggggtct cgggagaagc gcgcgccatg gccgtcaccg
    11581 cgatgaaaga aggcgtgagc cggttcaagc gggccctgat cctgggcgcg gcggcgaggc
    11641 tcttctccga gcgcggttat gccgcgacga ccatcgacgc catcaccgag gagctcaagg
    11701 cctcgcgccg cgcgatctac gatcatttcg tggggaagtc cgacatcctt gccgcgatct
    11761 gcgagcaggc agtccgcttc gcggccgagc tcgcggagcg cgtcgcccgc gagccgggcg
    11821 acccggcggc gaagctctcg cggcttgtgc gcgacttcac gctgatcgtc atcgagaacc
    11881 aggactacat cgccatctcc agccgcgaga tgaagtatct gccggaagag gcgcagcggc
    11941 gcatcttcca cctgcaggag cgcttcgacc gcatcctctc ggcgatgctt caggaaggcg
    12001 tgcgtgccgg cactctcgac actgccgatc ccgagatgac cggcctcgcc atcagcggca
    12061 tgatcatctg ggtccatggc tggtatcgtc cccgcggccg cctgtcgccg gcagagatcg
    12121 ccgagcgcat ggcggagacg gcgctgcgga tggcgcatgc gcggaaaggc gggcagcccg
    12181 ccgcggcgcg gtgacacgga gttcggcaaa ggcggcgccc ggaagaaaca aggaggcgct
    12241 cgccggcgac cagaaacgtc aagggaggag ggaatgctcg aaggcctgca gatgaatttg
    12301 ccgctcgcgg tgtcgtgtct gatcgaccat gcggcggcgt accacgcgga caccgaaatc
    12361 gtggcgcgca ccatcgaagg cgatgtgcac cgcacgacct atggcgcggt agggagccgc
    12421 accaagcgtc tcgccagagc gctgctgcga ctgggcgtgg cggccggcga ccgcatcggc
    12481 agcctcgcct ggaacaccca tcggcatttc gagatgttct acggcgtctc aggcaccggc
    12541 gccgtcctcc acaccatcaa tccgcggctt ttcgccgagc agctcgttta catcatcaat
    12601 cacgccgagg accggatcct cttcatcgat gcggcgacgc tgccggtctt cgagcagatc
    12661 gcgccgcttc tcaccacggt caggcaatgc gtgatgatgg cgccgcgcga gcgcatgccg
    12721 gcgaagagcg cggcgggcga tttcctttgc tatgaggagc tgctcgccgc cgaggacgac
    12781 gattatcaat ggcccgaatt cgacgagcgc gccgcctcga ccatctgcta cacctcgggc
    12841 accaccggaa atcccaaggg cgtcgtctac tcgcaccgca gcgccatgct gacgacgatg
    12901 ctgatcaaca gcatggtcgg cctgggctcg cgcaacggcg cacgggaggt gctcatgccg
    12961 atggcaccga tgttccacgg caatgcctgg cagttcccct atgtggcgcc gatggtgggc
    13021 gcgaagctgg tcctgcccgg acgcaactac gagccgcaga agctctatga gttgatcgaa
    13081 gaggaagggg tgacgatcac ctgcggcgtt ccgacgctat ggatcatgct cgccgactgg
    13141 ctcgaccgca ccaatcagcg cttctcgaaa ttgcggctga cgctgtcctc gggctcggcg
    13201 ccgccccgag cgctcgtcga aaagctcgag agcaactatg gcatcgaatt cgtccaggcc
    13261 tggggcatga cggaagcgct gggcggcagc aacgccctca tggagcccgg cagcgccgac
    13321 ctgcccttcg aggagcgcat cgaccgtcgc atgaattcgg ggcgtacgct ctggggcgtc
    13381 aagtaccgga tcgtcgacga cgaagagcgg gtcttgcccc atgacgggaa ggcctccggc
    13441 catttgcggg tgaaggggcc gtggatcgcc tccggctatt tcaaaggcga aggcggcagc
    13501 gcgctcgacc gggacggttg gctcaagacc ggcgacgtcg ccagcatcga tccgcgcggc
    13561 catatcctgc tcaccgatcg gtcgaaggac gtgatcaaat ccggcggcga gtggatcagc
    13621 tcgatcgcgc tcgaaggtgt cgccgccggt catcccgaag tgttgcaggc cgcggtgatc
    13681 ggcgtcaccc acccgaaatg gcaggaacgg ccgctgctgg tcgtggtgcg caagcagggc
    13741 agcaccctct ccggcggcgc gctgctcgac ttcatgaggg acaaggtcgc gacctggtgg
    13801 ctgcctgacg acgtcgcttt catcgacgcc atgcccatga ccgggaccgg caaggtgcac
    13861 aagctgacgc tcaggacgca gttcagggac tacgtcctgc cggacctcga gcgcgtgggt
    13921 cgtgcctcgc acggctgaga cgacgtcccc agttcggcga aaccgaccga agacgttgga
    13981 gcagccgcgc gatcccatac gaaaaagagc cccggcgcgc cgccggggct caggatgaac
    14041 aaggaggctt cacgtcaggg agactccagg gtccgaagac cctcttcccg gggctcgccc
    14101 caggaaaccg agaccgttca aaaacggccg aaaaccaacc cctattgcat aaaacgcgcg
    14161 ccgcgaaaac accatgcgag attttcgtcg gcacccctgc aaaaaccgca tcgctgcggg
    14221 gccagcgctg acttttccgc gatttatcag gcgataagct cgcccggatg acgcggcgac
    14281 tcagctgttc cgcccctccg tgaccttgcg cagcaggaaa tcgcggaaga cggcgatgcg
    14341 cttcgaagag cgcagctcct cggggtagac gaaataggcc tccacctcgg ggccggcgag
    14401 ctcgggcagg atacggacaa ggctcgccgc ttcctgaccc atgaaatcgg ggagtgccgc
    14461 gatgccgagc ccgctctcga ccgcgcggaa gatcgcgtag acattgttga ccatgaagat
    14521 gggctgccgg tcgatgccgg cgcgcgcgcc cacctcgcgc agccagttaa tgttgcgcac
    14581 gggctgccgg ctgtcctcgc cgtagacgat gaggcgatgc ttgtcgagat cctcgggttt
    14641 ctgcggcgtg ccgaagcgct tcagatagtc cgtcgaggcg tagacgcgat agcgcaccgt
    14701 catcagatgg cgctgcacca aatcgggctg gcgcggcggc gacatgcgga tcgcgacgtc
    14761 agcttcgcgc atcgacagat cgagctcgct gtcgtcgacg accagggtca cctggatctc
    14821 gggatagaga tcggtgaact cgcggatgcg cggcgtgagc caggccgagc cgaaggagac
    14881 ggtggtcgtc accttgagcg ggcctttcgg ccgatccttc gattccgaca gcatcgcctc
    14941 ggtcatggcg agcttcgaga agatctcgcg ggccgtgtga tagagcagct cgccttgctc
    15001 ggtgaggatc agcccgcggg cgtggcggtg gaaaagcgga acggaaaggc tttcctcgag
    15061 cgcgctgatc tgccggctca cggccgattg gctgaggttg agcgcctcgc cggcatgcgt
    15121 gaagctgccc gcttccgcga ccgcgtgaaa caccctgagc ttgtcccagt ccatgcccct
    15181 atcctgcagc atgtccctgt ccctgcatta ctcggccgct ttgagcgccg ccgccgcttc
    15241 ttccacggcg aggaatttct ccgcttcgag ggctgccatg cagcccatgc cggcggcggt
    15301 gacggcctgg cggaatacct tgtccttgac atcgcccgcg gcgaacacgc ccggcacatc
    15361 ggtgaccgtc gaatcgggtt tcgtcaggat gtagccgtcg cggtcgagag ccagctgacc
    15421 ccgcacgatg tcggtcaccg gcgtgtggcc gatggcgacg aagaaaccgt cgcacgggat
    15481 cacggagcgc gcgcccgtcc tgacattggc gatgcgcaca ccggtgaccc ggggcggcat
    15541 gccttcgccc agaatctcct cgacagcgct gtcccagacg atgtgaatct tgggattggc
    15601 gaagaggcgc gcctggagga tcttctccgc gcgcaaccga tcgcgacggt ggatcaaggt
    15661 gacgcgcgtc gcgtggttgg tgagatagag ggcttcctcg acggcggtgt tgccgccgcc
    15721 caccaccacc acctcgcggt cgcggaagaa gaagccgtcg caggtggcac aggcggagac
    15781 gccggcgccg cgaaacttcg tctcgctggg gatgccgagc cagcgcgcct gggcaccggt
    15841 gcaaagcgcc agcgtatcgc tgacatagat gtcgccggaa tcgccgacgc agacaaaggg
    15901 gcgcttcgag agatcgacgc tgacgataag gtcgctcatg aggcgggtgc cgacatgggc
    15961 ggcctgcttg gccatttgct ccatgagcca aggcccctgg atcacctcgc cgaagccggg
    16021 atagttctcg acatcggtgg tgatggtgag ctggccgccc ggctgcaaac cctgcaccag
    16081 aatcggctga agattggcgc gcgcggcata gatcgctgcc gtgaagccgg ccggcccgga
    16141 gccgatgatg agaaactttg tgtggtgccg ctgggtcatt tcgcaatgtt ccgcaaggcc
    16201 cgctcagaaa ccgaacatgt gatcgaggct gaacgcgccg cccttggcga gcagcccatg
    16261 gtaaccaaag aggcgacagg tcgtgtcccg gattatgata taggcaccgt cgcccgcgcg
    16321 gtcacggagg ccctggtcga acatctcgcc atagcgccag aacagcgacc ccgagcaggg
    16381 aatggcaacc tgccgccgcg ccagctcgcg gccgctgccg tcatggagga cgaggtcggt
    16441 ggcgctctcg tcatgccatg gcatcgatgc cggatagatc agatggcaga acgtgtcgtg
    16501 cggacgctcg ccgagccgca ggaagagccg cgtcgacagc cccggcgcgc gaccggcata
    16561 ggattgcggc tcgtccttgt aggtgagcac ggtattgaag acatgcgcac cgaagctggt
    16621 ctcggccatg tggccgctgg cgcgatcctc gtagcggaag aggccgtgca gccagccgtc
    16681 gccgctgccg ccgtcccgga agtcgtagac cagttcgaga tggccgtagc ccgagggcag
    16741 cgccgcgccg tcgatcacgc catcgaggtc gaccgcgacc gagtctcggc ggagcaagcg
    16801 gccgaagcga tggcgtgccg attcgcggcc cgcgccgtca tagacgacga gcgcgatggg
    16861 gaggtcttcc tgggtccgcg ccatcggcgt cggcagtgcc aggctgcgcc atgtcccccg
    16921 cggcaggatc ggtgccggca ggagatagcc ttttcccagc atgttgccga gctcagggag
    16981 gcgcggatcg ggcttgagat cggtgcgctc gacattgaca tgggcgatgc gccgcctgcc
    17041 gccccgcacg acctcgtaac gcggccgcac cacgtacttg ccggcgctca cctcgatctg
    17101 ctgcggccag cgcgcggccg gcaacatgct tgcaatgtcg accgcgtggc tcgcgaacgg
    17161 cgcgatctcc cgatcgatgg cggccagcgc cgcgtcgccc atgaggttga ggccgaccgt
    17221 ccccgccgga attgcgcagg gttggctgtt ctgcacccaa agcgtcaccg cctcgccaga
    17281 gtcgggcgcc ggcagtccgg catagtaatc ggcgggccac gaattggcgt catgggtgca
    17341 ggagaaacat gaggcggcat cgtcgccgaa ggtgtcgagc gcatatttca cgacgtcctg
    17401 gccgacggcg ccgatcgcat gcaggaacag ctgacccgtg aattccggga gcgcgaagcg
    17461 cgcgcgaatg gcgcggctgt cgagtatgat cgacgcggtc cccgcggcga cgggctcgct
    17521 ccattcggca agcaccttgc cttcggcatc gaacagcagc aaccagagcg tgagcgacga
    17581 cgcgccgtat cccgcccaat aattcgcggt gacgagccgc gtatgcaggc cggcctgatc
    17641 gcggaagaac acgaagttag tggcgaagtt gaccggatcg agatagcgtc ggcgattcgt
    17701 gagaagctcg tccggaagac gaatctcgtc gagagtcgcg acctcggcgc cgggcggcag
    17761 caggtggcgg atgtgaccaa ccgcgcgccc ggcatcgaac gccgcgacga gcatgcggcg
    17821 tgccggcgat tcgcgaagct cggtgacggg ctgcgcgcgc tgccccagga tctgacgtcc
    17881 gatcgccgtc acgtcctgga cgaagcagcc ggcgatgtcg agtcgcgaga gcggataaag
    17941 atcggaaagc ccatcggcga ggccgtccgg gtcatagacc gcgagcggac ttccgccgag
    18001 tcgacacagc agccgttcca tcgcgcgcgc ggccaatgga tgcgtcaccg ccttgaaaaa
    18061 ggcgttgccg ccacgattgt tgttgaaggt ttcgatcctc agaggcatgc gccatgactt
    18121 agcaaggggt ggcgacgaat actaggccgt tgtcaccgca acagcctatc atagaggtcg
    18181 gcccgtcagg actctcggca aggatcaggc ggatctccac gcgcggtcag ccatgacata
    18241 gagcagagcg agtctcctca aggagcgcaa agcacggtag gttcagccgt atggcggtgc
    18301 agagcgatag actgtcgcaa gcagcgatcg aggcgcggtc ctggcggtgg gcgctttggg
    18361 cagtgtttgc actcacgctg gcgcggctgg cgtggctgtc gctcggccgt gcggatctct
    18421 atcccgacga ggcacagtat tggctgtggt cgttgcaccc ggcgctcggc tactactcga
    18481 aaccgccgct ggtggcctgg ctcatcgccg cgacgacggc gttgttcggc gaggacgaat
    18541 tcgccatccg cctttcggcg ccgctgctgc atttcgctac gagcctcgtc atctatatgc
    18601 tggcgcggcg gctctatgac gcgcgtgtgg cgctgtggtc cgcggtggcg tatgcgacgc
    18661 tgccgggcgt ctccgcgtcg gcggcgctca tctccacgga tgcaccgttg ctgttgtgct
    18721 ggggcgtggc actctacgct ttcgtcaggg cgcgggagcc cggcggcggg cgatggtggc
    18781 tcgcggtggg agtcgccgcc ggcatcggcc tcctggccaa atacgccatg gtcttttggc
    18841 tgatgtcggc actgctcttc ctgctcctct tccgcgacga gcggcggcat gcgtcgcgct
    18901 ttctcggcgc caccgcgctc gcgctcgcga tctacgcgcc gaatttccta tggaacgcgg
    18961 caaacggttt cgtcagctat cgtcatacca gcgacaatgc cgcgttcggc gggcagcttc
    19021 tgcaccccct gcacttcctc gaattcttcg gctcgcaatt cgccgtgttc ggcccgatct
    19081 tcttcgcggt gctgatcttc ctggcgctga cggcgcgacg gactctggtg gagcgccgcg
    19141 ccgctctgct cgccgccttc gcgctgccga cgcttctcat catgctcgcg gagagctttc
    19201 tctcgcgcgc tcagcccaat tggtctgcgc cgaccttcat ttcggccgtg gttctggtgg
    19261 tcgcgtggct cgtcatcgag gggcggcaga cctggatcgt cgcgtcggtc gcgctgcacg
    19321 cgacggcagc ggtggtctgc ttcgccgccc atgacgtcag cgctgctctg ggcgcgccgt
    19381 tgccggccaa atatgacctg ctgcatcgcc tgcgcggctg gaaggtgctc gggcgcagcg
    19441 tgagccaaag tctcgccagc catccgggaa cgctgctgat gtcggatgat cgcgaggaca
    19501 tggcggcgct catctactat gtgcggccgc atcctttcga tgccctgaaa tggaacggcg
    19561 aaggcggcat ccatgacggc ttcgacc
//
LOCUS       DASYNS010000364         6668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1961403, whole genome shotgun sequence.
ACCESSION   DASYNS010000364 DASYNS010000000
VERSION     DASYNS010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961403"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VGP50_11370"
     CDS             complement(<1..1148)
                     /locus_tag="VGP50_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8028024.1"
                     /translation="MSKGRRGASGKHPAEGPAQPDGASAGRRRYVRRTDDHRRRDLIA
                     AAADWLWECSADGRINFLSPAFEASTGLQRRSVLGTRLSDLASADRAFAAADAQRAAI
                     AAQKPFRDLVFKLGEAVGPSAWLEIAGTPIVEAGAFRGYCGLGKTVTARIEAALALRN
                     SERRHREFFDVASDWFWETDPEGRVSYLSSNVEAVLGLPVSAYIGKRVAETEGIVIEP
                     KAGRANLAAFKARQPYRDFVYFRKLPNGKIVWVSSSGAPFYGEDGAFLGYRGIARDVT
                     GRIEAERALRGSELEVRQLLEAAADYNWEYDTQHRYVYMSPQFEALTGIPSAAVLGKR
                     LVELPGVSVDPEMGKMVLQAIKARQSFRDFVYSRKYPDGKVRWFKVSGA"
     gene            1395..2324
                     /gene="lpxC"
                     /locus_tag="VGP50_11375"
     CDS             1395..2324
                     /gene="lpxC"
                     /locus_tag="VGP50_11375"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEV8028025.1"
                     /translation="MDDDVGIALQKTLKSAIHCRGVALHSGARVAMTLRPAAPDSGIV
                     FRRSDCAGAEIKAEWRSAVEQPLCTRLDNGEGQSVGTVEHLMAALAGLEIDNAMIELD
                     GPEVPGMDGSAAPFVFLIDCAGVVEQDAPRRAIKVLKPVSVGVAGKSATLSPDDGFSM
                     SFAIDFASGAIGRQDISIAVDPDSFRADISRARTFGFLHEVDQLRAAGLARGSSLDNA
                     VVINGDQVLNREGLRYVDEFVRHKVLDALGDLYLAGAPLIGHFRGERSGHALNLRLIE
                     ALFADPTAWCMTSAPRRLPTPLAWEETAERARA"
     gene            2425..3237
                     /locus_tag="VGP50_11380"
     CDS             2425..3237
                     /locus_tag="VGP50_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEV8028026.1"
                     /translation="MTLAQRVRWLSFASCLLLAACGSTSKDDYVEKPVEDLYNSAMDQ
                     LNDESYDKAAKAFEEVERQHPYSVWATKGQLMAAYSLYLAGKYDDSIVACDRFIQLHP
                     GHRDIAYAYYLKALDYYVQIADVARDQKATQQSLDALSEVVRRFPDSKYGRDARLKID
                     LARDHLAGKEMEIGRYYERNGSPLAAINRFRRVVDEYQTTTHVPEALHRLTECYLALG
                     LTEEAQRTAAVLGYNYPGSPWYDNTYALLKTRAPTAIASSTAAKDDDATRDR"
     gene            3323..4996
                     /gene="recN"
                     /locus_tag="VGP50_11385"
     CDS             3323..4996
                     /gene="recN"
                     /locus_tag="VGP50_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV8028027.1"
                     /translation="MLLGLTIRDVVLIDRLDLAFHGGLCVLTGETGAGKSILLDALGL
                     AVGVRAESGLVRHAAEQAVVTAEFSLGPRHPARRLLADAGLDATGDTLVLRRVVAADG
                     RSRAFIDDQPASVGLLKQVGETLVEIQGQFEQQGLLDPATHREALDAYGVEAARNVAL
                     SAAWRQWRERASARDAAASALAKARADEDYLRHAVAELDALDPVPGEEARLAEERTLR
                     MNREKLGEALESALGELRGERGGEHALHSAQRQLERLKDKAGGRLDAAIAALDRAALE
                     AREAVTQIETASRGLDRDGASLDKIEERLFALKALARKHNVAVAGLPALRAEMTARIA
                     ALDDGGAAALRLARQAEEARREYVAAAERVGKERRAAAKKLDAAVMKEFAPLKLEKAR
                     FATVLTALEESEWGPSGAERVHFEVATNPGTLLGPLARIASGGELSRFMLALKVVMAR
                     TSPVPTLVFDEVDSGIGGAVAAAVGERLHRLGEELQVLVVTHSPQVAARGAHHWRVAK
                     QQAQARTLTKVEELSAEERCEEIARMLSGSAVTAEARAAAASLMAGARP"
     gene            5032..>6668
                     /gene="ligA"
                     /locus_tag="VGP50_11390"
     CDS             5032..>6668
                     /gene="ligA"
                     /locus_tag="VGP50_11390"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV8028028.1"
                     /translation="MSETQAKAELARLAETIAHHDRLYHEKDAPEISDADYDALRVRN
                     AAIERRFPQLVRADSPSRRVGAAPATGFAKVTHAKPMLSLDNAFDEEDVRAFFQSVRN
                     FFRRPADLARVDPATIAVAAEPKIDGLSCSLRYERGALVLAATRGDGVIGEDVTANVR
                     TLKDVPHRLTTRSWPDAIEIRGEIYMERPAFFALNAEREKAGEPVFANPRNFAAGSLR
                     QLDPAMTAQRPLKFFAYAWGESDRPFARTHSEAIRHFREWGLPVNPLSKLCHGVDEVL
                     AFHRAMAAQRASLPYDIDGVVYKVDDLELEERLGFAARAPRWAIAHKFPAEQAQTVLK
                     EILIQVGRQGTLTPVAVLEPITVGGVVVERATLHNEDEIKRRDIHEGDTVVIQRAGDV
                     IPQVVSVVLDRRPDKSAPYILSEVISRRSGKPECPICHSLAIREEGEAAWRCTGGLIC
                     PAQVVERLRHFVSRDAFDIDGLGMKHITAFWEDRLIKTPADIFRLDPAVIAEREGWGE
                     QSARKLHDAIELRRKITLDRFIYALGIPQVGQQTAKLL"
BASE COUNT         1099 a         2230 c         2270 g         1069 t
ORIGIN      
        1 gcaccgctga ccttgaacca gcgaaccttt ccgtcggggt acttccggga gtacacgaag
       61 tcgcggaagg attgccgcgc cttgatcgcc tggagcacca tctttcccat ctcggggtcg
      121 accgaaacgc cggggagctc cacgaggcgc ttgcccagca ctgccgccga cggaatgccg
      181 gtgagtgcct cgaactgggg ggacatgtag acgtagcggt gttgcgtgtc atattcccag
      241 ttgtaatcgg ccgctgcctc gagcagctgc ctgacctcga gctcgctgcc gcgcaaggcc
      301 cgctcggcct cgatccgccc cgtgacatcc cgggcgatgc cgcgatagcc gagaaaagcg
      361 ccatcctcgc catagaaggg cgcgccgctc gaactgaccc agacgatttt gccgttcggc
      421 aacttccgaa aatagacgaa gtcgcggtag ggttgccgcg ccttgaacgc cgcaaggttc
      481 gcccttcccg ccttgggctc gatgacgatg ccttcggtct cggccacacg cttgccgata
      541 taggcggaca ccggcaggcc gaggacggcc tcgacattgg acgagaggta gctgacgcgg
      601 ccctccggat ccgtctccca gaaccagtcg gaggccacat cgaagaactc gcggtggcgc
      661 cgctcgctgt ttcgcagcgc aagagcggct tcgatccggg cggtcacggt cttgccgaga
      721 ccacaatagc cgcggaaggc gccggcctcg acgatgggcg ttcccgcgat ctcaagccac
      781 gccgaagggc cgaccgcttc tcccagcttg aagacgagat cgcggaacgg tttctgcgcg
      841 gcgatggcgg cgcgctgcgc gtctgctgcc gcgaatgcgc ggtcggcgga ggcgaggtcg
      901 gagaggcgcg tgcccaggac agaccgccgc tggagaccgg tggatgcctc gaacgccggt
      961 gagagaaaat tgatgcgccc gtcggcgctg cattcccaaa gccaatccgc ggcggctgcg
     1021 atgagatcac gacggcggtg atcgtcggtg cggcgtacgt agcgtcgccg gccggccgac
     1081 gcgccgtcgg gttgcgccgg tccctcagcc gggtgtttgc cagacgcccc acgccgtccc
     1141 ttggacattc ctcgatccct gatggccgca aagtgcgggt cgtcgatgat gccatcgagt
     1201 gattgcgcga agcttagcga cccctccttt gctgcactgc ggcaaaccga atagttaatg
     1261 gatcgtaacc tgttgattct acggcgtttt taatgaaaca atctgaaaca aagagtgatt
     1321 tgtcgaagcg gccgcgcgtg gttagtttac gcgtgccgca acaggggggc cgcagcatct
     1381 tgactgatat gatcatggac gacgacgtgg ggatcgccct gcagaagacg ctgaaatccg
     1441 cgatccattg ccgtggcgtc gcgcttcact cgggggcgcg cgtcgccatg acacttcgtc
     1501 ccgccgcgcc cgacagcggc atcgtctttc gccgcagcga ctgcgccggg gccgagatca
     1561 aggcggagtg gcgcagcgcg gtcgagcagc ccctctgcac ccgcctcgac aatggcgagg
     1621 gccagtcggt ggggacggtc gagcatctca tggcggcgct cgccggcctc gagatcgaca
     1681 atgccatgat cgagctcgac gggcccgaag tgccgggcat ggatggcagc gcggcgcctt
     1741 tcgtcttcct gatcgattgc gccggcgtgg tcgagcagga cgcgccgcgc cgggccatca
     1801 aagtgctgaa gcccgtgagc gtcggcgtcg ccgggaaatc ggcaacactc tcgcccgatg
     1861 acggcttctc catgagcttc gccatcgatt tcgccagcgg cgccatcggc cggcaggaca
     1921 tctcgatcgc cgtggatccc gacagcttcc gggccgacat cagccgcgcc cgcaccttcg
     1981 ggttcctcca tgaggtcgac cagctgcggg cggcgggcct cgcgcgcggc agttcgctcg
     2041 acaacgcggt ggtcatcaat ggcgaccagg tcctcaatcg cgaggggctg cgctatgtcg
     2101 acgagttcgt gcgccacaag gtgctcgacg ctttgggcga tctctatctg gccggcgcgc
     2161 cgctgatcgg tcatttccgc ggcgagcgct cgggccatgc gctcaatctc cgcctcatcg
     2221 aggctctctt cgcggatccg acggcttggt gcatgaccag cgccccgcgc cggctgccga
     2281 cgccgctcgc ctgggaagag accgccgagc gcgctcgcgc ctgacctttt cgtcgcgatt
     2341 tccaagagca aaggtggtcg tccgacccgg cgccatgcta taagggcgcg tgcgtctctt
     2401 ttgggtgctg gtcgggtgcg aataatgacg ctggctcagc gtgttcgatg gctctccttc
     2461 gcttcttgct tgttgctcgc cgcctgcggc agcacgagca aggacgacta cgtcgagaag
     2521 ccggtcgaag acctctacaa ctccgcgatg gaccagctca acgacgagag ctacgacaag
     2581 gcggccaagg ccttcgagga agtcgagcgc cagcatcctt actcggtgtg ggccaccaag
     2641 gggcagttga tggcggccta ttcgctctat ctggcgggga aatacgacga ctcgatcgtc
     2701 gcctgcgacc gcttcatcca gcttcatccg ggccaccgcg acatcgccta cgcctattac
     2761 ttgaaggcgc tcgactatta cgtacagatc gccgacgtgg cgcgcgacca gaaggcgacc
     2821 cagcagtcgc tcgacgcgct cagcgaagtc gtccggcgct tccccgacag caagtatggc
     2881 cgcgacgcgc ggctaaagat cgatctcgcg cgcgaccatc ttgccggcaa ggagatggag
     2941 atcggccgct attacgagcg gaacgggtcg cctctcgccg cgatcaatcg cttcaggcgc
     3001 gtggtcgacg agtaccagac gaccacccat gtgccggagg cgctgcatcg cctcacggaa
     3061 tgctatctgg cactcggcct gaccgaggag gcgcagcgga cggcggcagt gctcggctac
     3121 aactatccgg gcagtccgtg gtacgacaac acctacgcct tgctgaagac gcgcgctccc
     3181 acggcaattg cgagttcgac ggccgccaag gacgacgacg ccacccgcga ccgttagcgg
     3241 cgccagttcc gctgttctct ctatcctgtt gcctgcgcgc gcaagttgac cccgtgccgc
     3301 cgtctcggca tggtgctggc cgatgctgct cggcctgacc atccgcgacg tcgtgctgat
     3361 cgatcggctc gatctcgcct ttcacggcgg gctgtgcgtc ctcaccgggg aaaccggcgc
     3421 cggcaagtcg atcctgctcg acgccttggg cttggcggtg ggcgtccgcg ccgagagcgg
     3481 cctcgtgcgc catgccgccg agcaggccgt ggtcaccgcc gaattctcgc ttggcccccg
     3541 ccacccggcg cggcgcctcc tcgccgatgc cggtctcgac gccacgggcg acaccctcgt
     3601 gttgcggcgc gtggtcgcgg ccgacggacg cagccgcgcg ttcatcgacg accagcccgc
     3661 gagcgtgggt ctcttgaagc aagtcggcga gacgctggtc gagatccagg gtcagttcga
     3721 gcagcagggt ctgctcgatc cggcgaccca tcgcgaagcg ctcgacgcct atggcgtcga
     3781 ggcggcgcgg aacgtggcat tgtcggcggc atggcggcag tggcgggagc gcgcaagcgc
     3841 gcgtgacgcc gcggcgtcgg cgctcgccaa ggcgcgcgcc gacgaggact atctgcgcca
     3901 cgccgtggcg gagctcgacg cgctcgatcc ggttcccggc gaagaggcgc ggttggcgga
     3961 ggagcgcacg ctccgcatga atcgcgagaa gctcggcgag gcgctcgagt ctgcgttggg
     4021 cgagcttcgg ggcgagcggg gcggcgagca cgcgcttcat tcggcgcagc gccagctcga
     4081 gcggctcaag gacaaggccg gggggcggct cgacgcggcg atcgcggccc tcgaccgcgc
     4141 cgctctcgag gcccgcgagg ccgtgacgca gatcgagacg gcgtcgcgcg gcctcgaccg
     4201 ggatggcgcg agcctggaca agatcgagga gcggctgttt gcgctgaagg cgctggcccg
     4261 caagcacaat gtggccgtcg cgggcctccc ggcgctgcgt gccgaaatga cggcgcggat
     4321 cgccgcgctc gacgacggcg gcgctgctgc cctgcgcctc gcgcgtcagg ccgaggaggc
     4381 acggcgggaa tatgtggccg cggccgagcg cgtcggcaag gagcggcgcg ccgcggcgaa
     4441 aaaactcgac gctgccgtga tgaaggagtt tgctcctttg aagctcgaga aggcgcggtt
     4501 cgcgacggta ctgacggcgc tcgaggagag cgaatggggc ccgtccggcg ctgagcgcgt
     4561 gcatttcgag gtcgcgacca atccggggac gctgctcggc ccgctcgcgc ggatcgcctc
     4621 gggcggggag ctgtcgcgct tcatgctcgc gctcaaggtg gtcatggcca ggacctcacc
     4681 ggtcccgacg ctggtcttcg acgaggtcga cagcggcatc ggcggtgccg tggccgcggc
     4741 cgtcggcgag cgtctccacc gccttggcga ggagctgcag gtgcttgtgg tcacgcattc
     4801 gccgcaagtc gccgcgcgcg gcgcacatca ctggcgcgtc gccaagcagc aggcccaggc
     4861 gcgcaccttg acgaaggtcg aggagctgtc ggccgaggag cgctgcgagg agatcgcgcg
     4921 catgctgtcg ggaagcgctg tcacggccga ggcgcgcgcc gccgcggcga gcctgatggc
     4981 gggagcgcgg ccgtgacgcg tgggcggagc gcgccgcccc cggtcgagga gatgagcgag
     5041 acccaggcca aggccgagct cgcgcgactg gccgagacca tcgcccatca cgaccggctc
     5101 taccacgaga aggacgcgcc cgagatctcg gacgcggatt atgacgcgct gcgcgtgcgc
     5161 aatgcggcga tcgagcggcg gttcccgcag ctcgtgcgcg cggacagccc gtcccgccgc
     5221 gtcggcgccg cgcccgcgac cggcttcgcc aaggtcacgc atgcgaaacc catgctgtcg
     5281 ctcgacaatg ccttcgacga ggaagacgtc cgcgccttct ttcagagcgt gcgaaatttc
     5341 ttccgccggc cggcggacct cgcgcgcgtc gatccggcga cgattgccgt cgcggccgag
     5401 cccaagatcg acgggctctc ctgctcgctg cgctacgagc gcggcgcgct ggtgctggcg
     5461 gcgacgcgcg gcgacggcgt catcggcgag gatgtcaccg ccaatgtgcg aacgctgaaa
     5521 gacgtgccgc accgcctcac gacccgttcc tggcctgacg ccatcgagat ccgcggcgaa
     5581 atctatatgg aacggccggc gttcttcgcc ctcaacgccg agcgcgagaa ggcgggcgag
     5641 ccggtcttcg ccaatccgcg caatttcgcc gcgggatcgc tgcgccagct cgatcccgcc
     5701 atgacggcgc agcggccgct caaattcttc gcctatgcct ggggcgagag cgaccgcccc
     5761 ttcgcccgga cgcacagcga ggcgatccgg catttccggg aatggggcct ccccgtcaat
     5821 ccgctgtcga agctttgtca cggcgtcgac gaagtgctgg cgtttcaccg cgccatggcc
     5881 gcgcaacggg cgtcattgcc ttatgacatc gacggcgtgg tctacaaggt cgacgacctc
     5941 gagctcgaag agcggctggg cttcgccgcg cgcgcgccgc gctgggcgat cgcgcacaaa
     6001 ttccccgccg agcaggcgca gaccgtgctc aaggagatcc tcatccaagt cggacgccaa
     6061 ggcaccttga cgcccgtcgc cgtgctcgag ccgatcacgg tcggcggcgt tgtcgtcgaa
     6121 cgcgcgacgt tgcacaacga ggacgagatc aagcgcaggg acatccacga gggcgatacc
     6181 gtcgtgatac agcgcgccgg cgacgtgatc cctcaagtcg tctcggtcgt ccttgaccgc
     6241 cgtcccgaca aaagcgcgcc ctacatattg tcggaggtga tatcacgacg cagcggcaag
     6301 ccggaatgtc ccatctgtca tagcctcgcc atacgcgaag aaggagaggc ggcgtggcgg
     6361 tgtaccggtg gcctgatctg cccggcccag gtcgtggagc gcttgcggca tttcgtatca
     6421 cgcgacgcct tcgacatcga cggcctcggg atgaagcaca tcacggcgtt ctgggaagac
     6481 aggctcatca agacgccggc cgacatcttc cgcctcgatc cggcggtaat cgccgagcgc
     6541 gaaggctggg gcgagcagag cgcccggaag ctccacgatg cgatcgagct gcggcgtaag
     6601 attacgctcg accgattcat ctatgcgctg ggaattccgc aggtcgggca acagacggcg
     6661 aagctcct
//
LOCUS       DASYNS010000365         5385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5081972, whole genome shotgun sequence.
ACCESSION   DASYNS010000365 DASYNS010000000
VERSION     DASYNS010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5081972"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VGP50_11395"
     CDS             <1..471
                     /locus_tag="VGP50_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028029.1"
                     /translation="GQVKIESTPGCGTTVRLYLPRLGSGDRAGDMGGDAPETSAQRLD
                     ATILVVEDDEDVRRHAVGILRELGYRVVETADGPAALRLLGAEPRIDMLFADVELPGG
                     LDGHALAERARALRPELKVLLTTGYVRSTDAEAGLIPKPFTHAALAARIGRLLE"
     gene            complement(605..1099)
                     /gene="ssb"
                     /locus_tag="VGP50_11400"
     CDS             complement(605..1099)
                     /gene="ssb"
                     /locus_tag="VGP50_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV8028030.1"
                     /translation="MAGSVNKVILVGNLGRDPEIRSTQDGMRIANLSVATSESWRDKG
                     SGERKERTEWHRVVIFNEKLVEIVEKYLKKGSKIYVEGALQTRKWTDNSGQERYSTEV
                     VLQRFRGDLTMLDTRSGGGGSGGYADEAPAEEFATAGAGTGGSAPRSERRPPVQDLDD
                     EIPF"
     gene            1269..2702
                     /locus_tag="VGP50_11405"
     CDS             1269..2702
                     /locus_tag="VGP50_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV8028031.1"
                     /translation="MSHPPKPSYDQAAENLVGALSRKLGTIGLEVHEAASVINDVATQ
                     FERQEAQAKRLRESAQLMAGANRAIDDATATAHAAAESGQSELESSHRVVAQAVAQVG
                     ALADAVERVEKRLAAIGVSLDEVAGISSSIEAIARQTNLLALNATIEAARAGAAGKGF
                     AVVAGEVKALAGQTRQATLKIGATVDTLSGQIASLLSDSTSAAKGAKATRQGAQVIEQ
                     AVDRVSQSFSKVAELSGTIAASARVNLQHCNTLITEVDTLDADIAASSVKVRKADDRF
                     AEVLDLVAQAVDEIATAGVRTADTPYLDATKELAARIMASFDEALERGEITMAELFDE
                     NFVAIPNTNPQQFLTKYTEMCDRRLPAIIEPYLQLLPNVQYALGIGPQNYLPTHHRQY
                     SKPQGPDPAWNAANCRNRMMHKHQRTFTVAKNRANDGDDKIGSVRLFTRRRDFGAGQY
                     QMVKTAVSPLWIRGRHWGPASIGYTLA"
     gene            2789..3799
                     /locus_tag="VGP50_11410"
     CDS             2789..3799
                     /locus_tag="VGP50_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV8028032.1"
                     /translation="MSDYFPISILDSSDSEAQPAAPAAAPEPELLDAYSRAVVGAAEK
                     VGPAVLHLQIEGANGRHGGAGSGVVFTPDGYVLTNSHVAGGARHIEATFPDGRSVGAR
                     LVGDDPDTDLAVLRLDGEAPAFARLGDARKLRVGQLVIAIGNPFGFQATVTAGVVSAL
                     GRSLRARSGRLIDSVIQTDAALNPGNSGGPLVTAAGEVVGINTAIIAAAQGICFSISA
                     NTVEFVAARLIREGRVRRSYIGLAGQNAPLPRRVVRFHSLERDSGVRVDSTSADGAAR
                     AAGLLSGDIVVGFAGEAVATVDDLHRLLTEERVGKPAVITVLRRAEKREFTITPRDRP
                     QG"
     gene            4003..>5385
                     /locus_tag="VGP50_11415"
     CDS             4003..>5385
                     /locus_tag="VGP50_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HEV8028033.1"
                     /translation="MQFIRVRGAREHNLKNIEVDLPRDSLVVITGLSGSGKSSLAFDT
                     IYAEGQRRYVESLSAYARQFLELMQKPDVDSIEGLSPAISIEQKTTSKNPRSTVGTVT
                     EIYDYMRLLFARIGVPYSPATGLPIESQTVSQMVDRLVALPEGTRIYLLAPIVRGRKG
                     EYKKELADLQKRGFQRVKVDGKMLEIDAVPALNKKLKHDVAVVVDRVVLRADLGTRLA
                     DSLETALELADGLAIAENADTGEETTFSAKFACPVSGFTIPEIEPRLFSFNNPFGACP
                     TCDGLGTKLFFDPELVVPDESKSLREGAVAPWAHSSSQYYAQTLDSLARHYKASTNEP
                     WRDLPEKVRNAILFGSADTPVTMKYDDGVRAHTITRPFEGVIPNMERRFAETDSAWLK
                     EELGRFQNTRACESCHGKRLKPEALAVKIRGIDISVVADLSIADAARWFGELADHLTP
                     KQRDIAQRVLK"
BASE COUNT          903 a         1879 c         1753 g          850 t
ORIGIN      
        1 ggccaggtga agatcgaaag cacgcccggc tgcggcacga cggtgcggct ctatctgccg
       61 cggctcggca gcggcgatcg cgccggcgac atggggggtg atgcgccgga gacatcggcg
      121 cagcgtctgg atgcgacgat cctcgtcgtc gaagacgacg aggacgtgcg gcgccacgcc
      181 gtcggcatcc tgcgcgagct cggctatcgc gtggtcgaga cggccgacgg acctgccgcc
      241 ttgcggctgc tgggcgccga gccgcgcatc gacatgctgt tcgccgatgt cgaacttccc
      301 ggcgggctcg acggccatgc cctggccgag cgcgcccggg cgctccgccc cgagctcaaa
      361 gtgctcctca ccaccggcta tgtccgcagc accgacgccg aggccgggct gatccccaaa
      421 cccttcaccc atgccgctct cgccgcccgg atcgggcggt tgctggagta gcctccgccg
      481 ccattaccaa ggcgaggatc agaagtcagc gcacgcttgg caccgtcccc tcaccctccc
      541 gttgctacgc aacgggtccc tccctctccc gcaagcggga gagggggaag atgcgcctct
      601 ccgattaaaa cggtatctcg tcgtcgaggt cttggacggg ggggcggcgt tcgctgcggg
      661 gagcgctgcc gccggtgccg gcgccggccg tggcaaattc ctccgcgggg gcttcgtcgg
      721 catagccgcc actgccgccg ccgccgctcc gcgtatcgag catggtgaga tcgccgcgga
      781 agcgctgcag caccacttcg gtgctgtagc gctcctggcc ggaattgtcg gtccatttgc
      841 gcgtctggag cgcgccctcg acatagatct tcgagccctt cttcagatac ttctcgacga
      901 tctcgacgag cttctcgttg aagatcacga cgcggtgcca ctcggtgcgc tccttgcgct
      961 cgccgctccc tttgtcgcgc cagctctccg aggtggcgac ggacaaattc gcgatgcgca
     1021 tgccgtcctg agtggagcgg atctccggat cccggccgag attgccgacg agaattactt
     1081 tattaacgct tccggccatg gtgctgcctg ccttccgttg ctgctatcgg acggctctgg
     1141 cgcgccgcat aagccatctt agccgcggcc gatagagaag cccatccctc gcggcttcgg
     1201 aacataacag gaactcatgg gtccggcaaa gggaaaatta aaccgaagtg gctaaaattc
     1261 tctcggtcat gtcacaccct ccgaaaccct cctacgacca agccgcggaa aacctggtcg
     1321 gcgcgctcag ccgcaagctc ggcaccatcg gtctcgaagt gcatgaagcc gccagcgtca
     1381 tcaacgacgt ggccacgcag ttcgagcgcc aggaggcgca agccaagcgg ctgcgcgaga
     1441 gcgcgcagct catggcgggg gccaaccgcg cgatcgacga cgccaccgcc accgcccatg
     1501 cggcagccga gtccgggcag agcgagctcg aaagctccca ccgcgtcgtc gcgcaagcgg
     1561 tcgcccaggt cggggcgctg gcggatgcgg tggagcgcgt cgagaagcgg ctcgcggcga
     1621 tcggcgtttc gctcgacgag gtcgcgggca tctcgagctc gatcgaggcg attgccaggc
     1681 agaccaacct cctcgcgctc aacgccacca tcgaagcggc gcgcgccggg gcggccggca
     1741 agggttttgc cgtggtcgcg ggcgaggtca aggcgctggc gggccagacc cgccaggcga
     1801 cgctcaagat cggcgccacc gtcgacacgc tctcgggaca gatcgcgagc ctgctcagcg
     1861 attcgaccag cgccgccaaa ggcgccaagg cgacgcgcca aggcgctcaa gtcatcgagc
     1921 aggcggtcga ccgcgtcagc cagagcttct cgaaagtggc cgagctcagc ggcaccatcg
     1981 ccgcctcggc ccgcgtcaat ctccagcatt gcaatacgct gatcaccgag gtcgacacgc
     2041 tcgatgccga catcgccgca tcctcggtca aggtgcgcaa ggccgatgac cgcttcgccg
     2101 aggtgctcga tctggtggcg caagcggtcg acgagatcgc aacggccggc gtgcgcacgg
     2161 ccgacacgcc ctatctcgac gcgacaaagg agttggcggc gcggatcatg gcgagcttcg
     2221 acgaggcgct cgagcgcggc gagatcacca tggccgagct tttcgacgag aatttcgtcg
     2281 ccatccccaa taccaatccg cagcagttcc tcacgaaata caccgagatg tgcgaccgcc
     2341 ggctgccggc gatcatcgag ccttatttgc agctcctgcc gaacgtccaa tatgccctcg
     2401 gtatcgggcc gcaaaactac ctgccgacgc accatcggca gtactcgaag ccgcaaggtc
     2461 ccgatcccgc gtggaacgcc gccaactgcc gcaaccggat gatgcacaag catcagcgca
     2521 ctttcacggt ggccaagaac cgggcgaatg acggcgacga caagatcggc tcggtgcgcc
     2581 tcttcacgcg acggcgcgac ttcggcgccg gccaatacca gatggtgaag acggctgtct
     2641 cgccgctctg gatccgcggc cgccactggg ggccggcctc gatcggctac acgctcgcct
     2701 aggccgcctt cgccctgcgc cgtggacgaa ggcgccgtgc gcctccgcct ctgggcagcg
     2761 ggcaccgggc tccctatcta tgggccccat gagcgactat ttcccgatct ccattctcga
     2821 ttcttccgac agcgaggctc agcccgcggc gcctgccgcc gcgccggagc cggagctgct
     2881 cgatgcctat tcgcgcgccg tggtcggcgc cgccgagaaa gtcggtcccg ccgtccttca
     2941 cctccagatc gagggcgcga acggacgcca cggcggcgcc ggctcgggcg tcgtcttcac
     3001 gcccgacggc tatgtgctga ccaacagcca cgtcgcgggc ggtgcccggc atatcgaggc
     3061 gaccttcccc gacggccgca gcgtcggggc gcgcctcgtc ggcgacgatc ccgacaccga
     3121 cctcgcggtg ctgcgcctcg acggcgaggc gccggccttc gcgcggctgg gcgatgcgag
     3181 gaagctccgc gtcgggcagc tcgtcatcgc catcggcaac cccttcggct ttcaggcgac
     3241 cgtgacggcg ggcgtggtga gcgcgctcgg ccgcagcctg cgcgcgcgct cgggccgcct
     3301 catcgacagc gtcatccaga ccgacgccgc gctcaacccc ggcaactccg gcgggccgct
     3361 ggtgacggcg gcgggcgaag tcgtcggcat caacacggcg atcatcgcgg cggcccaggg
     3421 aatctgcttc tcgatctcgg cgaacacggt cgagttcgtc gccgcgcggc tcatccgcga
     3481 aggccgggtg cggcgctcct atatcggcct cgccggccag aacgcgccgc tgccgcgtcg
     3541 cgtcgtgcgt ttccacagcc tcgagcgcga cagcggcgtg cgtgtcgact cgacctcggc
     3601 cgatggcgcg gcgcgcgccg ccggcttgct ctcgggcgac atcgtcgtcg gcttcgccgg
     3661 cgaagccgtt gccacggtcg acgacctgca ccggctgctg accgaggagc gtgtcggcaa
     3721 gcccgcggtg atcaccgttc tgcgccgggc ggagaagcgc gaattcacga tcacgccgcg
     3781 cgaccggccg cagggttgaa agaaaagaga gattgaccag tgaggcggtg aggcagtgag
     3841 aagaagatgt tggcacgctc tgcgcgccga atcgtcttct tttttcctct cactgcctca
     3901 ctgcctctgt ggtcgatctt gcttttctgt ccgcctcgga ggggtggcgt cgccccggcg
     3961 atggaagcta tagtgacggc accagccacc ggctcatccc tcatgcaatt catccgcgtc
     4021 cgcggcgcgc gcgagcacaa tctcaagaat atcgaggtcg acctgccgcg cgacagcctc
     4081 gtcgtgatca cggggctgtc gggctcgggc aagtcgtcgc tcgctttcga cacgatctac
     4141 gccgaggggc agcgccgcta tgtcgagagt ctctcggcgt atgcgcgcca gttcctcgag
     4201 ctgatgcaga agcccgacgt cgattcgatc gagggcctct cgccggcgat ctcgatcgag
     4261 cagaagacga cgtcgaagaa cccgcgctcg acggtgggca cggttaccga gatctatgac
     4321 tatatgcgcc tcctcttcgc gcgcatcggc gtcccctact cgcccgccac gggcttgccg
     4381 atcgaaagcc agacggtgtc gcagatggtc gaccggctgg tcgctttgcc cgaggggacg
     4441 cggatctatc tccttgcgcc gatcgtgcgg ggacgcaagg gcgagtacaa gaaagagctc
     4501 gcggatctgc agaagcgcgg cttccagcgc gtcaaggtcg acggcaagat gctggagatt
     4561 gacgccgtgc cggcgctcaa caagaagctc aagcacgacg tcgccgtggt ggtggaccgc
     4621 gtcgtgctgc gcgccgatct cggcacgcgt ctcgccgatt cgctggagac ggcgctcgag
     4681 ctcgccgacg ggctcgccat cgccgagaac gccgacaccg gcgaggagac gaccttctcg
     4741 gcgaaattcg cctgcccggt ctcgggcttc accatccccg agatcgagcc gcggctcttc
     4801 tccttcaaca atccgttcgg cgcctgcccc acctgcgatg ggctcggcac caagctgttc
     4861 ttcgatccgg agctggtggt gccggacgaa agcaagtcgc tgcgcgaggg cgcggtcgcg
     4921 ccctgggcgc attcgagctc gcaatactac gcgcagacgc tcgacagcct cgcgcgccac
     4981 tacaaggcca gcaccaacga gccgtggcgc gacctgccgg agaaggtgag aaacgccatc
     5041 ctcttcggct cggccgacac gccggtcacg atgaaatacg acgacggcgt ccgcgcgcat
     5101 acgatcacgc gccccttcga gggcgtcatc cccaacatgg agcggcgctt cgcagaaacc
     5161 gacagcgcct ggctcaagga ggagctgggc cgcttccaga atacgcgggc ttgcgaatcc
     5221 tgccacggca agcggctgaa gcccgaggcg ctcgctgtca agatccgcgg catcgacatc
     5281 agcgtggtcg cggatctctc gattgccgac gcggcgcgct ggttcggcga gctcgcggat
     5341 cacttgacgc cgaaacagcg cgacatcgcg cagcgcgtgc tcaag
//
LOCUS       DASYNS010000366         2784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3368813, whole genome shotgun sequence.
ACCESSION   DASYNS010000366 DASYNS010000000
VERSION     DASYNS010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3368813"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1711)
                     /locus_tag="VGP50_11420"
     CDS             complement(89..1711)
                     /locus_tag="VGP50_11420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028034.1"
                     /translation="MSFLDEFFSSAALSPHGFCLLWRPALIWLHVASDTVIGVAYYTI
                     PVALGYFVWKRGDIVFGWMFWMFAAFILACGTTHWFEVWTLWHADYGTQGLIKAVTAV
                     LSLGTATLIWPLLPRAIALPSAADLRRVNEELSVQVRERNQAVEALQREMVERQHAED
                     ALRQSQKLEALGQLTGGVAHDFNNLLLILGTNLYLLREQLGGTEIEPQLASMERAIAR
                     AESLTRHLLTFGRRQALQPRPTELRGQIAKLADLLRRSLREDIEIRLDVPPEIWPIEI
                     DPGEFELAIINLALNARDAMPESGVLSIGASNETLPRNADAAEALEGDFVAITVNDTG
                     LGIPPDIVARVFEPFFTTKDVGKGTGLGLSQVYGFAKQSGGDAIVRSEEGRGTTVTLF
                     LPRATRAPSVTTEIAGSDVAPPHDGKKATVLLVEDNADVAEVSAALLGGLGYIVRVAT
                     SAQTALDRFAAGETADLVFSDVVMPGGMNGLDLARTLRTRYPGLPVLLATGFGTQQQV
                     VQSGFPLLLKPYRAKALASAIETLLHAGRCVS"
     gene            complement(1846..2118)
                     /gene="infA"
                     /locus_tag="VGP50_11425"
     CDS             complement(1846..2118)
                     /gene="infA"
                     /locus_tag="VGP50_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV8028035.1"
                     /translation="MAKEDLIEFDGTVVELLPDARFRVRLDNGHETLAYSSGKMKKNR
                     IRILTGDRVTVEMTPYDLTKGRINFRHKDERAAPASPRPSFPRRGR"
     gene            2375..2761
                     /locus_tag="VGP50_11430"
     CDS             2375..2761
                     /locus_tag="VGP50_11430"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV8028036.1"
                     /translation="MGVALNHIIVHAKDRWASARFLAGILGLAAGPAWGPFVPVRTAN
                     GVTLDFADARDFRPQHCAFLVSEEEFDAALARIKAAGVGFYASFDGTGRGEINRLYGG
                     RGVYFDDPDGHLFELITQPYGAVPEG"
BASE COUNT          468 a          931 c          922 g          463 t
ORIGIN      
        1 tcgccctggt cgcgccacag cacccaattc tcgacgacgt cgcggatgcg ctggcgttgc
       61 gtcatgtcgc cgtccatcgc gccctctctc aagagacgca gcggccggca tgcagcaggg
      121 tctcgatcgc gctcgccagc gccttcgccc gatagggctt gagcagcagc gggaagccgc
      181 tttgcaccac ttgctgctgc gtgccaaagc cggttgcgag cagcaccgga agccccggat
      241 agcgcgtccg gagcgtgcgc gccagatcga ggccgttcat gcctccgggc atcaccacgt
      301 cggagaagac gaggtcggcg gtctcgccgg cggcgaacct gtcgagtgcc gtctgcgccg
      361 atgtcgcgac gcgcacgata taaccgaggc cacccagcag cgcggcgctt acctcggcga
      421 catcggcatt gtcctcgacc agcagcaccg tcgctttctt gccgtcatgg ggcggcgcca
      481 cgtcgcttcc ggcgatttcg gtcgtcaccg acggcgcgcg cgtggcccgc ggcaggaaca
      541 gcgtcaccgt cgtgccgcgc ccctcttcgc tgcgcacgat ggcatcgccg ccggattgct
      601 tggcgaagcc atagacctgg ctcagaccca gccccgtgcc cttgccgaca tccttcgtcg
      661 tgaagaacgg ctcgaagacg cgcgcgacga tgtccggcgg gatgccgaga ccggtgtcgt
      721 tgacggtgat cgcgacgaaa tcgccctcga gcgcctcggc ggcgtcggcg ttccgcggga
      781 gcgtctcgtt gctcgcgccg atcgacagca cgccgctctc gggcatggcg tcccgcgcat
      841 tgagcgcgag attgatgatc gccagctcga actcgccggg atcgatctcg atcggccaga
      901 tctccggcgg cacatcgagg cggatctcga tgtcctcgcg cagcgaccgg cgcagcaggt
      961 ccgccagctt cgcgatctgg ccgcgcagct cggtcgggcg cggctgcagg gcctggcgcc
     1021 ggccgaaggt caggaggtga cgtgtcaggc tctcggcccg ggcgatggcg cgctccatcg
     1081 acgcgagctg tggctcgatt tcggtgccgc ccagttgctc gcgcaggaga taaaggttgg
     1141 tgccgaggat cagcagcaga ttgttgaagt catgcgcgac gccgccggtg agctggccca
     1201 gcgcctcgag cttctgcgac tggcgcagcg catcctcggc gtgctgccgc tcgaccatct
     1261 cacgttgcag cgcttcgacc gcctgattgc gctcgcgcac ctgcacggag agctcctcat
     1321 tgacccgccg cagatcggct gccgagggca gggcgatcgc ccgcggcagc agcggccaga
     1381 tcagcgtggc ggtgccgagc gacagcaccg ccgtcaccgc cttgatcagg ccctgcgttc
     1441 catagtcggc gtgccagagc gtccacacct cgaaccaatg agtcgtgccg caggcgagaa
     1501 tgaaggcggc gaacatccag aacatccagc cgaagacgat gtccccgcgc ttccacacga
     1561 aatagccgag cgccaccggg atggtgtaat aggccacgcc gatgaccgtg tcggaggcca
     1621 catggagcca gatgagtgcg ggccgccaga ggaggcagaa gccatggggc gacaaggctg
     1681 cggacgagaa gaattcatcg aggaacgaca tggccgacct cactccaaga caacttgggg
     1741 tgacctcacc atgccatcaa aggcgcgagg tgccaatatt ggccataata ggcaattgac
     1801 ggctctctcg ccgcggttgt agcgccggag gaagtgctct gcggcctacc gcccgcgccg
     1861 gggaaagctg ggccgtggcg acgccggcgc cgcgcgctcg tccttgtggc ggaaattgat
     1921 gcggcccttg gtgagatcat agggcgtcat ctcgactgtg acgcgatcgc cggtcaaaat
     1981 gcggatgcga ttcttcttca tcttgccgga gctgtaggca agcgtctcat gaccattgtc
     2041 gagcctgacg cggaagcgcg cgtcgggcag cagctcgacg acggtgccgt cgaattcgat
     2101 caaatcttcc tttgccacag agcctccatt cgctgggata acggatgccg cgctgccggc
     2161 caacgccgct acagagcagc gcccggagga taagcgccgg ccggcaaatt gcaaccggcg
     2221 cgtctgcgca agattcggcc catgcgcgag ccctgacacg gtcttgcgct tgggatattc
     2281 tgccgcgtcc gcccggtacc ggcactttca gaagacgcgc ctgccggcgc atgtagagct
     2341 ggcctgcgcc aacgtttcga cggggagatc gaggatgggc gtcgcgctca accacatcat
     2401 cgtccacgcc aaggaccgct gggcgtctgc ccggttcctc gctgggattc tcggattggc
     2461 ggcgggtccc gcctgggggc ccttcgtccc ggtccggacc gccaacggcg tcacgctcga
     2521 tttcgccgat gcgcgggatt tccgtcccca gcactgcgcc ttcctcgtga gcgaggaaga
     2581 gttcgatgct gcgctcgcgc gcatcaaggc cgcgggcgtg ggattctatg ccagcttcga
     2641 cggcaccggc cgcggcgaga tcaaccgtct ttatggcggc cgcggcgtct atttcgacga
     2701 tcccgacggc catcttttcg agctgatcac gcaaccctac ggagccgtgc cggagggctg
     2761 aggaaagatc agggcgacga cgcg
//
LOCUS       DASYNS010000367         2313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3524909, whole genome shotgun sequence.
ACCESSION   DASYNS010000367 DASYNS010000000
VERSION     DASYNS010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3524909"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1513
                     /locus_tag="VGP50_11435"
     CDS             134..1513
                     /locus_tag="VGP50_11435"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEV8028037.1"
                     /translation="MADGDEDSLPGRMRRYARVSTAMGGLAARLAGERYLGFPLDRAR
                     HAADLKAALGGIKGPLMKVAQLLATIPEALPKEYASELAVLQANAPPMGWAFVRRRMA
                     SELGQGWRTKFKRFEHEAARAASLGQVHRAETFDGRLIACKLQYPEMASAVEADLSQL
                     KLVMSIYERYDRAITLAEIHAEIAERLREELDYGREAAHMRLYRRMLAEERAVHVPEP
                     LPELSTTRLLTMTWLEGMPFLEAAKAPAEQRNIIARNMFRAWYVPFYYYGVIHGDPHL
                     GNYTVRTDGSVNLLDFGCVRIFPPPFVKGVIDLYHALDRDDEALAVSAYECWGFRDLS
                     REMIAVLNRWANFLYAPLLDDRKRRIQEKEGAYGRDIAEEVHHEIRRLGGVQPPREFV
                     FMDRAAIGLGSVFMHLKAEINWHRLFHELIEDFDVATLAKRQRQALAAAGVPGYQGEP
                     AAPRKRRRS"
     gene            complement(1510..>2313)
                     /locus_tag="VGP50_11440"
     CDS             complement(1510..>2313)
                     /locus_tag="VGP50_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV8028038.1"
                     /translation="THCYIESSPRNDRLAYLSLAEMQGYLDEIAQDRLPTEEIGFTGG
                     EPFMNPDIMAMLEACLARGFRVLVLTNAMRPMQRLQAKLLDVQRRHGERLTIRVSLDH
                     YTRELHEEERGPDTWQPTIDGLKWLVVHGFTTAVAGRTLWDEPDCAMRQGYELLFAEH
                     GIPIDAADPQRLVIFPEMDIAADVPEITEACWGILHVDPASPMCASSRMVVKRKDAAR
                     PSVVACTLLPYDEAFDLGPTLAGAAGAVPLNHPHCAKFCVLGGGSCSPG"
BASE COUNT          383 a          772 c          781 g          377 t
ORIGIN      
        1 gacgcacggg tgggtgtggg gtgccaacgc acatggtcct gaccaaggcc ccctcaccct
       61 cccgtcgctg cgcgacgggc ccctccctct cccacaaggg gagagggaat actcagcgtc
      121 cgcgtaacga agcatggccg acggcgacga ggactcgctg ccggggcgga tgcggcgcta
      181 tgcgcgggtg tcgacggcca tgggcgggct cgcggcgcgg ctcgccggcg agcgctatct
      241 cggatttccg ctcgatcgcg cccggcatgc ggcggatctg aaagcggcgc tcggcggcat
      301 caaagggccg ctgatgaagg tagcgcagct tctcgcgacc atccccgaag cgctccccaa
      361 ggaatatgcg agcgagctcg cggtgctcca ggccaacgcg ccgccgatgg gctgggcctt
      421 cgtccgccgg cgcatggcga gcgagctcgg ccagggatgg cggacgaaat tcaagagatt
      481 cgagcacgag gcggcgcgcg cggcgtcgtt gggccaggtg catcgcgcgg aaacgttcga
      541 cggcaggctg atcgcctgca agctgcaata tcctgagatg gcctcggcgg tcgaggcgga
      601 tctctcgcag ttgaagctgg tgatgtcgat ctatgagcgc tacgaccgcg ccatcaccct
      661 cgccgagatc catgccgaga tcgcggagcg gctgcgcgag gagctcgatt acggccgcga
      721 agccgcgcac atgcggctct atcgccggat gctggccgag gagcgcgccg tccacgtgcc
      781 ggagccgctg cccgaactgt cgacgacgcg ccttctcaca atgacctggc tcgagggcat
      841 gccgtttctc gaggcggcga aggcgccggc cgagcagcgc aacatcatcg cccgcaacat
      901 gttccgcgcc tggtacgtgc ccttctatta ctacggcgtc atccacggcg atccgcatct
      961 cggcaactac accgtgcgga ccgacggcag cgtcaacctt ctcgatttcg gctgcgtgcg
     1021 catctttccc ccgcccttcg tcaaaggcgt catcgatctc tatcacgcgc tcgaccgcga
     1081 tgacgaggcg ctcgccgtct ccgcctatga atgctggggt tttcgcgacc tgtcgcgcga
     1141 gatgatcgcg gtcctgaacc gctgggcgaa ttttctctac gcgccgctgc tcgacgaccg
     1201 gaagcgccgc atccaggaga aggaaggcgc ctatggccgc gacatcgccg aggaggtcca
     1261 tcacgagatc cgccgcctcg gcggcgtcca gccgccgcgc gaattcgtgt tcatggaccg
     1321 cgccgctatc ggcctcggct cggtcttcat gcatctgaag gccgagatca actggcaccg
     1381 cctcttccac gagctgatcg aggatttcga cgtcgcgacc ttggcgaaac gccagcgcca
     1441 ggcgctcgcg gcggccggtg tgccgggata ccagggcgaa cccgcggcgc cgcggaagcg
     1501 gcgccgctcc taacctgggc tgcaggaccc tccgccgagc acgcagaatt tggcgcaatg
     1561 cggatgattg agcggcacgg cgccggccgc gccggcgagc gtcgggccga ggtcgaaggc
     1621 ttcgtcgtaa ggaaggagcg tgcaggcgac caccgacggc cgcgccgcgt ccttgcgttt
     1681 cacgaccatg cgcgagctgg cgcacatcgg cgaggccggg tccacatgga ggatgcccca
     1741 gcaggcttcc gtgatctcgg gcacgtcggc ggcgatatcc atttccggga agatcacaag
     1801 acgctgggga tctgccgcat cgatagggat gccgtgctcg gcgaagagca gctcatagcc
     1861 ttgccgcatg gcgcaatcgg gctcgtccca gagcgtgcgc cccgccaccg ctgtcgtgaa
     1921 gccgtggacg acgagccatt tgaggccgtc gatcgtcggc tgccaggtgt ccgggccgcg
     1981 ctcctcctca tggagttcgc gcgtgtagtg gtcgagcgag acccggatgg tcagccgctc
     2041 accgtgccgc cgctgcacgt cgagaagctt ggcctgcagc cgctgcatcg gccgcatggc
     2101 gttggtcagc accaggacgc ggaagccgcg cgcgaggcag gcctcgagca tcgccatgat
     2161 gtccgggttc atgaaaggct cgccgccggt gaagccgatt tcttccgtcg gcaggcgatc
     2221 ctgcgcgatc tcgtcgagat atccttgcat ctccgccagc gagagatagg ccaggcgatc
     2281 gttgcgcgga ctcgattcga tatagcaatg cgt
//
LOCUS       DASYNS010000368        16139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4149485, whole genome shotgun sequence.
ACCESSION   DASYNS010000368 DASYNS010000000
VERSION     DASYNS010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 16139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16139
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4149485"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..524)
                     /locus_tag="VGP50_11445"
     CDS             complement(75..524)
                     /locus_tag="VGP50_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEV8028039.1"
                     /translation="MADARDLSKELARGLCRALAQRGFVTLTEVPLATGRRADVLALG
                     REGELVIIEIKSSVADFKSDRKWPEYRQYCDRLYFAVPDGFPRELIPEDCGLIGADPF
                     GGAVLREAPLLPLAAARRRAMLLRFARIAAGRLRRLLDPAIDDPELG"
     gene            608..2332
                     /locus_tag="VGP50_11450"
     CDS             608..2332
                     /locus_tag="VGP50_11450"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEV8028040.1"
                     /translation="MTTSKSSVLREAWRIARPYWFSEERWLARGLLAAVLIMSLAQVW
                     INVRLNSWRNDFYNTLQNYDEPGFLYQVFLFTLLVGAFVALFVFGNYLQQLLQIRWRR
                     WMTTVYLREWLSDKVYYRLQLAGDGTDNPDQRISEDLNWFPAQTLNLSIGLLTNLVQA
                     VSFSFILWDLSGPLVLSVGGQTLSIPGYMFWAVLIYTAAATGLAIYLGRPLIRLNFAQ
                     QRLEADFRFSLVRLRENTESIAFYGGEPREMETFSRRFHGIYQNYLAIMRRMLILGSA
                     QNGTGQAAVVFPYLIMAPRFFGERLLIGAIQQLADAFIQLQSSLAYIVTAYNDPNPLI
                     SITNWLAVVQRLSTFRDRVDEIQAALRAPQPIKMERAGRGITVSGLELDLPDGRMLRK
                     DVDLDVAPSEALLIRGPTGTGKSTLLRAIAGLWPFGRGRIRLDEGRAFFLPQKPYIPL
                     GDLRQALLYPDAGTGVRTEQLVDVLNKVGLGHLAAALDTVDLWAQRLSGGEQQRLAIA
                     RVLLAQPAIIFLDEATASLDEAGQATLYRLLRDLPWRPTIVSVGHRSTLPQFHDRVFD
                     LAVQQPAE"
     gene            complement(2339..2509)
                     /locus_tag="VGP50_11455"
     CDS             complement(2339..2509)
                     /locus_tag="VGP50_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEV8028041.1"
                     /translation="MTALLSNLGLDESGATAIEYSLIAGLISLVVLGWATFTGTTLTS
                     VFENIAQSLALA"
     gene            complement(2736..3479)
                     /locus_tag="VGP50_11460"
     CDS             complement(2736..3479)
                     /locus_tag="VGP50_11460"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028042.1"
                     /translation="MLQIRRLFKQFDDLTALADIDLAIDRGEIVGISGCGKSTLLRIV
                     AGLEAPSLGSVVLDGVAVAGPRPEVGLVFQEPRLMPWLDVRANVEFALARRPRAQRRP
                     LADAALARVGLTAFADALPQLLSGGMAQRAAIARALVARPAVLLLDEPFSALDAFTRF
                     DLQDHLLEIWRADEPTMLFVTHDIEEALVLSDRVVVMRAKPGRVYAQYRIELPRPRRR
                     TEAPLQSWKERILWDLDLAKKGPEPEFAI"
     gene            complement(3442..4287)
                     /locus_tag="VGP50_11465"
     CDS             complement(3442..4287)
                     /locus_tag="VGP50_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8028043.1"
                     /translation="MTEAIARIGRLALRRGALGLVVPALLLALWETLSRSGAAPPNLL
                     PPPSGVLRALRDLWVSGELPGHIAITLLRVLLGFLLGTAAATVLGAATGYSALWRRIL
                     DPLLQALRSIPSIAWVPLFVLWLGIFEASKVTLIGVGAFFPVYLNTMAGIQQVDRKLV
                     EVARVHGYGGVGLVRRVLLPAMLPAYVTGLRGGLGLAWMFVVAAEFMGASEGLGFLLI
                     DGQQTGRPANIIAAILLFAVFGKLSDVALARTARRFVAWQDSFESAEEKKANAANPAP
                     LQAIR"
     gene            complement(4284..5273)
                     /locus_tag="VGP50_11470"
     CDS             complement(4284..5273)
                     /locus_tag="VGP50_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV8028044.1"
                     /translation="MPISVLRSLLALGAALTAALPALAADQPSSVRVDWAYYNPVSLV
                     LRDKGTMEEEFAKDGIKIEWVLSLGSNKALEYLSGNSVDFGSTAGAAALLAKANGVPI
                     KAVYIYSKPEWTALVTGKDSAIRKVEDLRGKRIAVTRGTDPHIFLLRALALHGLKSQD
                     VKMVLLQHPDGKVALERGDVDAWAGLDPYMAQTELEQGSRLFFRDPDLNTYGFLNTRE
                     DFAAAHPDLVERVLAVYEKTRRWALAHPDELRVIVAKAGKLSDAVAAKDLERNDLSSG
                     MIGARQRDAIVAAGDVLKESGVIPADTDIAKTADALIDPQYITRVVARQEAEK"
     gene            5506..6852
                     /locus_tag="VGP50_11475"
                     /pseudo
     CDS             5506..6852
                     /locus_tag="VGP50_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057843149.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            7043..8524
                     /gene="lysS"
                     /locus_tag="VGP50_11480"
     CDS             7043..8524
                     /gene="lysS"
                     /locus_tag="VGP50_11480"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV8028045.1"
                     /translation="MADSDQDNQFRAARLEKLKALRALGVDPYPYAFARNAEAKALEG
                     RYAALPPGAMTEDRVAVAGRIRALRNSGLFIDLHDETGKIQIFSHKDFLPPAELPIVK
                     LLDLGDIIGVEGVVRRTPRGELTVNATHLAVLTKSLLPLPEKYHGLADVELRYRQRYL
                     DLIMNEESRATLRQRSRVVETIRRLLAERGFVEVETPMLQTIPGGAAARPFITHHNAL
                     DIDLYLRISPELYLKRLLVGGLSDKVFELNRNFRNEGLSPRHNPEFTLLELYQAYVDY
                     TAMMEITEFLVTQSAQAVRGALRLSYAGKEIDLTPPWPRRTMAELVQEHAGIDFLAIG
                     DDAGARSAARRIGIELADTTRWGHALYAVFESRVETLLVQPIHVTDFPRDVSPLTKVH
                     RADPRLTERFETFVNGWEIANAYSELNDPQDQLARFAAQMRERELGDEEAQRLDADFV
                     TALEYGMPPAGGLGIGIDRLVMLLTDSPSIRDVIAFPTMRPKE"
     gene            complement(8576..8872)
                     /locus_tag="VGP50_11485"
     CDS             complement(8576..8872)
                     /locus_tag="VGP50_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028046.1"
                     /translation="MNARMSSSGRRRLILGAFATSLALAGLAGGFAAAPARADDRDHR
                     DDARDDHRRDNHRVVRDRRVYVAPRPAYVYAPPPVYAPPPPPVGVDLVFPIHIR"
     gene            complement(8900..9442)
                     /locus_tag="VGP50_11490"
     CDS             complement(8900..9442)
                     /locus_tag="VGP50_11490"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEV8028047.1"
                     /translation="MMQILDPAARMLAAATVLGAMVVAGPLTAVAADQGPSSTVQLAQ
                     ANSTTAPPSASTKKKPRHARADVEDRIKNLHDSLKITPDQEQQWQAVADAMRADDKEM
                     KGVIADRRQHRDEMNAVDDLRNYQKVAETHVQGLQRLIPAFQTLYDSMSPEQKKNADT
                     VFGHHRHARASHASMQKPQQ"
     gene            complement(9554..10444)
                     /locus_tag="VGP50_11495"
     CDS             complement(9554..10444)
                     /locus_tag="VGP50_11495"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEV8028048.1"
                     /translation="MSALFGYLAYFLPAVIVLAVIEAAVLAIRRKGDYDWRAALASLA
                     DGLGREYIVYPFLGFSFAYPFLVWVSAHRLATLPVDGRLAFALLFIGVEFCYYWFHRA
                     SHRVRWFWTTHAIHHSSNELNLSAAYRFGWSIRLTGGGIFYAPLVWVGFPIPAVFAVV
                     SLNLLYQFWLHADWIPKLGWLEYVFNTPSHHRVHHAANPEYLDANYGGTLIVFDRLFG
                     TLIEERADLPCRYGLVKPLLSNNPFVINFHEWRALGRDLWQARSWRERLHYLFDAPGW
                     RPEGRGTTTEDLRRAAVAAE"
     gene            complement(10622..11926)
                     /locus_tag="VGP50_11500"
     CDS             complement(10622..11926)
                     /locus_tag="VGP50_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV8028049.1"
                     /translation="MPDDSRLICAEKVCKTYQTAETAGRLVLDHVDFALHEGEIVAIL
                     GKSGSGKSTFLRVLAGLTPPTEGHVAYRGQTVEGPAHGVAMVFQTFALFPWLTVLGNV
                     ELGLEAQGVPRAERRHRALQAIDLIGLDGFESAYPKELSGGMRQRVGFARALVVNPDV
                     LLLDEPFSALDVLTAETLRGDLMDLWLQRRVPTKGIILVSHNIEEAVEIADRIIIFGS
                     DPGHIRAEIPVPLPRPRDWASPRFRQIVDEVYALLTTAPGREGGKRGQRPEPIGIGYR
                     LPGASIQQLVGMLEALAAPPYSGRADLPHLVESEHIEDEALFVLIEALQLLGFAQTER
                     GDIWETPVGRDFAEADLQKRKQIFAERLKSGIPLAAHIRKVLDERPGHSAPEGRFIAE
                     LEDHLSEEEAREVFETVINWGRYAELFAYDYDRGVLSLENPT"
     gene            complement(11941..13677)
                     /locus_tag="VGP50_11505"
     CDS             complement(11941..13677)
                     /locus_tag="VGP50_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV8028050.1"
                     /translation="MEQRWGLPIVAWPSRLRPNVWDGVALPLVLGAVVLVAWASQQMN
                     VPYRLGEALPISLDPRNLPEYGLRTVLRMALALAASLAFSLSYAALAAKSRRAEKLLI
                     PVLDILQSVPILGFLSITVTGFVALFPGRLLGYECAAIFAIFTSQAWNMTFSLYQSFI
                     AVPQDLREAARMYHLSAWQSFWQLEVPFALPNLIWNMMMSVSGGWFFVVASEAISVAG
                     QDVKLPGIGSYIALATEQRDWGAVGYAILAMLVFILIYDQLLFRPLLAWAQRFKVEAI
                     GADEVEPPWFLTMLQRARLFDLLRWLVDYTALLASRVQRAMPRPARGSRPSLVAPRWL
                     DLAWNTALLAGTLVALGYIIGFVRSEVDVREVAHVVLLGFITLLRVVVLIALASLVWV
                     PIGVWIGLRPGLAHRVQPVVQFLAAFPANLFFPVVVSTIVAFHLNVEIWLSPLMILGT
                     QWYILFNVIAGTLALPTDYQFVASNLGVSRWLWWRRLILPGIFPAFVTGAVTASGGSW
                     NASIVAEIAKWGDTTLVATGLGAYIAQQTEKGDFPRIALGITVLSLYVLLFNRLLWRR
                     LYQLAEERLRLD"
     gene            complement(13834..14136)
                     /locus_tag="VGP50_11510"
     CDS             complement(13834..14136)
                     /locus_tag="VGP50_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEV8028051.1"
                     /translation="MSLEDATAAIRAKATTSPSLGHSIQFDLGADGIIHWDGTQTPPA
                     IDNTARDAETTITITLDDLKRLIAGALNPTMAYMTGKLKIQGSMGIALKMSQLLED"
     gene            14286..15125
                     /gene="cysE"
                     /locus_tag="VGP50_11515"
     CDS             14286..15125
                     /gene="cysE"
                     /locus_tag="VGP50_11515"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV8028052.1"
                     /translation="MSSIERSVHDVEAPQQAIAPQTPGELWLALRAEAVRSGERETAL
                     RDFLELAVLRHRDFAEALAALLAHKLAERSMPLERLEDIVRAAFAEQPQIIAAAIADL
                     AAIRSRDPAAGTPLTPFLYYKGFHALEWHRVGHWLWRGGRRDLAHFLQSRVSEVFAVD
                     IHPAVPVGSGVFIDHGTGLVVGETAVIGDDVSILQEVTLGGTGKERGDRHPKVRDGVL
                     LAAGAKVLGNIEIGRGAKVGAGSVVLYDVPPCATVAGVPARIVGWCDDGPPALAMDQS
                     LPE"
     gene            complement(15197..15724)
                     /gene="sodC"
                     /locus_tag="VGP50_11520"
     CDS             complement(15197..15724)
                     /gene="sodC"
                     /locus_tag="VGP50_11520"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HEV8028053.1"
                     /translation="MRIVLSLVCGLVLCGAAAAEAATAKVTVNKIDENGVGQSIGTLR
                     LKDTAKGLSITPKLKDLPPGPHGFHVHANGDCGPGEQNGKQVAGFAAGGHFDPDKTGK
                     HLGPDSSDGHRGDLPVLVVNDKGHAGKTVVAPRLKVADVKGHAIIIHANGDNYSDQPA
                     PLGGGGARIACGVVK"
BASE COUNT         2781 a         5358 c         5376 g         2624 t
ORIGIN      
        1 cgtgctcgcc gagctgctgc ccggtcatag caccaccgca accatcgccc gcctcgcgcg
       61 ctgaggccgc gcgctcagcc gagttcggga tcgtcgatcg ccgggtcgag cagccgccgc
      121 agccgtccgg cggcgatgcg cgcgaagcgc aggagcatgg cgcggcgccg cgccgcggcc
      181 agcggcagca agggtgcttc gcgcagcacg gcgccgccga acgggtccgc cccaatcagg
      241 ccgcaatcct cggggataag ctcgcgcgga aagccgtcgg ggacggcgaa gtagagccga
      301 tcgcagtatt ggcgatattc cggccatttg cggtcgcttt tgaaatcggc tacggacgac
      361 ttgatctcga tgatgacgag ctccccctcc cgcccgagcg ccagcacatc ggcacgccgt
      421 cccgtcgcga gcggcacttc cgtgagcgtg acgaagccgc gctgcgccag cgcgcggcag
      481 aggccgcgcg ccagctcctt cgagagatcg cgggcatccg ccatgccgca aagttttgcg
      541 cgacccccgc tggccgatca agtcccgcca tgacggtgcg gactcgccga ttgcggagaa
      601 cagcaacatg acaacgtcga agagcagcgt cttgcgcgag gcgtggcgca tcgcccgccc
      661 ctattggttc tcggaagagc gttggctggc gcgcggcctg cttgccgcgg tgctgatcat
      721 gagcctggcc caggtgtgga tcaacgtccg gctcaactcc tggcgcaatg atttctataa
      781 cacgctgcag aattacgacg aacccggctt tctctatcag gtctttttgt tcacgttgct
      841 cgtcggcgcg ttcgtcgcct tgtttgtttt cggcaattat ctgcagcagc tcctgcagat
      901 tcgttggcgt cgctggatga ccacggtcta tctccgcgaa tggctcagcg acaaagtcta
      961 ctatcgcctg cagcttgcgg gtgacggcac cgacaacccc gaccagcgta tttccgagga
     1021 cctgaactgg tttcccgcgc agacgctcaa cctctcgatc ggtcttctga cgaatctcgt
     1081 ccaggcggta tctttctcct tcatcctctg ggatttgtcg ggaccgctcg tcctctcggt
     1141 cggcggacaa acgctcagca tccccggcta catgttctgg gccgtgctca tctacacggc
     1201 agcggcgacc gggctcgcca tatatcttgg ccggccgctg atccggctga atttcgcgca
     1261 gcagcgtctc gaagccgatt tccgcttcag cctcgtgcgc ctgcgcgaga acaccgaaag
     1321 catcgccttc tatggcggcg agccgcgcga gatggagact ttttctcgcc gctttcatgg
     1381 aatctatcag aactacctgg cgatcatgcg gcgcatgctc attctcggtt ctgcgcaaaa
     1441 tggcaccggt caagccgccg tggtgtttcc ctacctgatc atggcgccgc gttttttcgg
     1501 ggaacggctg ctcatcggcg cgatccagca actcgccgac gccttcatcc agctgcagag
     1561 ctcgcttgcc tacatcgtca ccgcttacaa cgaccccaat ccgttgatca gtatcaccaa
     1621 ctggctcgcg gtggtgcagc ggctttcgac cttccgcgac cgcgtcgacg agattcaggc
     1681 ggcgctgcgc gcgccgcagc cgatcaagat ggagcgcgcc ggacgcggca tcaccgtcag
     1741 cggcctcgag ctcgatcttc ccgatgggag gatgctgcgg aaagatgtcg atctcgacgt
     1801 cgcgcccagc gaggcgctgc tcatcagagg gccgaccgga accggcaaga gcacgttgct
     1861 gcgcgccatc gccgggctct ggccgttcgg tcgcgggcgt atccggctcg acgagggccg
     1921 cgccttcttt cttccgcaga agccttacat ccccttgggg gacctgcgcc aagccttgct
     1981 ttatcccgac gccgggaccg gtgtgcggac ggagcagctc gtcgatgtct tgaacaaggt
     2041 ggggctcggc catctcgccg cggcgctcga tacggtcgat ctctgggcgc agcgtctctc
     2101 ggggggcgag cagcaacgtc tcgcgatcgc gcgggtgctt ctggcgcagc cggcgatcat
     2161 cttcctcgac gaggcgacag cctcgctcga cgaagcggga caggcgacgc tttatcgatt
     2221 gctgcgcgat ctaccctggc ggccgacgat cgtcagcgtc gggcaccgca gcaccttgcc
     2281 gcagttccac gaccgcgtgt tcgatctcgc tgtgcagcaa cccgccgaat aaccgggatc
     2341 acgcgagcgc gagactctgg gctatgttct cgaagaccga ggtgagcgtc gtgccagtga
     2401 aggtcgccca gccgaggacg acgagcgaga tcagccccgc gatcaagctg tattcgatgg
     2461 cggtcgcccc gctctcgtcc aaaccgagat tgctcaacag cgccgtcatg gattctgcct
     2521 cctgaggaca atccgccaag cgaaactacg ccccgatctc acgctaacgt tatcatggaa
     2581 ccgtgaccca ttgcagccac gatcagttca atctttatta aagcgccttc cacccctgtg
     2641 tctcagccat aatgtcatga ggtcgtaaac tttcacccaa gacgccgcct tcctttcgcg
     2701 gtgaccttgg taaaaattgc gtcaaatgcg ggagcctaaa tcgcgaattc cggctccggg
     2761 cctttcttgg cgagatcgag atcccagagg atccgttcct tccagctctg gagcggcgct
     2821 tcggtgcggc ggcgcggccg cggcagctcg atccggtatt gcgcatagac gcggcccggc
     2881 ttggcccgca tcacgacgac gcggtcgctc agcaccagcg cctcctcgat gtcgtgcgtg
     2941 acgaacagca tggtgggctc atcggcgcgc cagatctcga ggagatggtc ctgaaggtcg
     3001 aagcgcgtga aggcgtcgag tgcgctgaag ggctcgtcga gcagcagcac cgccggccgc
     3061 gccaccaatg cccgggcgat ggccgcgcgt tgcgccatgc cgcccgagag aagctgcggc
     3121 aaggcgtcgg cgaaagcggt gaggccgacc cgcgcgagcg cggcgtcggc gagcggacgg
     3181 cgctgcgccc gcggccgccg cgccagcgcg aattcgacat tggcgcgcac gtcgagccac
     3241 ggcatcaggc gcggctcctg gaagacgagg ccgacttcgg gccgcggacc cgccacggcg
     3301 acgccgtcga gcacgacgct tcccaaagac ggcgcctcga gtcccgcgac gatgcgcaac
     3361 agcgtgctct tgccgcagcc gctgatgccg acgatctcgc cgcgatcgat cgcgaggtcg
     3421 atgtcggcga gcgccgtcag atcatcgaat tgcttgaaga ggcgccggat ttgcagcatt
     3481 ggctttcttt tcttccgcgc tctcgaagga gtcctgccag gcgacgaagc ggcgcgccgt
     3541 ccgcgccagc gccacgtcgc tgagcttgcc gaaaaccgcg aagagcagga tggcggcgat
     3601 gatgttggcg ggccggcccg tctgctggcc gtcgatcagc aggaatccca atccctcgct
     3661 cgcccccatg aattcggcgg cgacgacgaa catccaggcg agccccagcc cgccgcgcaa
     3721 gccggtgacg taagccggga gcatcgccgg cagcagcacg cggcgcacga ggcccacgcc
     3781 gccatagcca tggacgcgcg ccacttcgac gagcttgcgg tcgacctgct gaatgccggc
     3841 catggtgttg agatagacgg ggaagaaggc gccgaccccg atcagcgtca ccttcgacgc
     3901 ctcgaagatg ccgagccaca gcacgaagag cgggacccag gcgatcgagg ggatgctgcg
     3961 cagcgcctgg agcagcggat cgaggatgcg ccgccacagc gccgaatagc cggtggccgc
     4021 gcccagcacc gtggcggcgg cagtgcccag caggaagccg aggagcacgc gcagcagcgt
     4081 gatcgcgata tggcctggga gctcgcccga gacccagaga tccctgagcg cccgcagcac
     4141 gcccgacggc ggcggcagca ggttgggcgg cgcggcgccc gagcgcgaca gcgtttccca
     4201 caaggcgagg agcagcgccg gcaccacgag cccgagcgcg ccgcggcgca gcgccagcct
     4261 gccgatgcgc gcgatcgcct cggtcatttc tccgcctcct gccgggcgac gacgcgcgtg
     4321 atgtattgcg gatcgatgag cgcatccgcg gtcttggcga tgtcggtatc ggccgggatg
     4381 acgccgctct ctttcagcac gtcacccgcg gcgacgatgg cgtcgcgctg ccgggcgccg
     4441 atcatcccgc tcgagaggtc gttgcgctcg agatccttgg cggccaccgc gtcgctgagc
     4501 ttgccggcct tggcgacgat gacgcgcagc tcatcgggat gggcgagcgc ccagcggcgc
     4561 gtcttttcat aaacggcgag cacgcgctcc accaggtcgg ggtgcgcggc cgcgaaatcc
     4621 tcgcgcgtgt tgaggaagcc ataagtgttg agatcggggt cccgaaagaa caggcgcgaa
     4681 ccctgctcga gctcggtctg cgccatatag gggtcgagcc cggcccaggc atcgacgtca
     4741 ccgcgctcga gcgcgacctt gccatcggga tgctggagca gcaccatctt cacatcctgt
     4801 gacttgagtc cgtggagcgc caacgcccgc agcaggaaga tgtgcggatc ggtgccacgc
     4861 gtcacggcga tgcgcttgcc gcgcagatcc tcgaccttgc ggatggccga gtccttgccc
     4921 gtcacgagcg cggtccattc cggtttcgag taaatgtaga cggccttgat cggcacgcca
     4981 ttggccttgg cgagcagggc cgcggcgccg gccgtcgacc cgaaatccac gctgttgccg
     5041 ctcaggtatt cgagcgcctt gttgctgccc aggctcagca cccattcgat cttgatcccg
     5101 tccttggcga attcctcctc catcgtgccc ttgtcgcgca gcacgaggct caccgggttg
     5161 taataggccc agtcgacgcg cacgctcgac ggttgatcgg ccgccagcgc cggcagcgcg
     5221 gccgtgagcg ccgctcccag cgccaggagc gagcgtagaa ccgagatcgg catcggtcgc
     5281 ctcctcaaac aaccgccgca gcttccgtcg ggcagggtgc cgtcgtcaac aagcgagatg
     5341 gcggttgcgc tttgccgcca aagccctaca accgccgccg agctctcata accctctccc
     5401 cccagtgagc ccgtgaatca tttcgcgggc tctacggatt gtgttgcgcg caccggattt
     5461 cgatgattcc ttgtgggaga ggtgattaag aggggtggtg ccgtgatgac gggtgaggac
     5521 ggacttttcg agggcttgcc cgagcaggtg gcgccgaagt ccagtcgcag cgagggagag
     5581 ccgcggctgc ggcgaccgga gcgtgatcag atcgagttgc gcgcggtcga cctcgatagc
     5641 ctgctggcgc aggaccatcc ggcacggatc atctgggcct atgtcgagcg gttggacatt
     5701 ggcgcgctgt atgcgccgat caaggcgcgg gagggaaccc cgggtcatcc gccgatcgat
     5761 ccgcggctga tgttggcgct gtggctctat gccacggctg acggggtcgg cagcgcacgg
     5821 gcgctggcgc gactgtgcga aagccaagac gcctatcgct ggctgtgtgg tggagtgagc
     5881 gtgaactacc acattctggc agacttccgg ctggcgcatc cgacgcttct cgacgagttg
     5941 ttggcgaaga acgtggcggc gctggcggct gccggcgtga tcgatctgga taccctggcg
     6001 caggacggag ttcgggtgcg cgcggcggcg ggcacctcgt cgtaccgtcg gcgggccagc
     6061 gtggagcggc ggctggagca ggcgcgagcc gtggtcgcgt agctgaagcg cgaggtagag
     6121 caggaccccg aggcaaacgc gcggcgtgtc aaggcggcgc gcgagcgcgc cgcacgcgag
     6181 cgcgcggcgc gtgccgaggc ggcgctggcg aagctcgatg agatcgaggc gcaacgcgcg
     6241 cgtcgcgcga agacgcatgc caagaagacc gccaagcaga gcgagccacg ggtttccctg
     6301 accgatccgg aggcgcgcat tatcaagatg cccgatggcg gtttccggcc ggcctacaac
     6361 atgcagattg tcagcgccgt ggagagtcag atcatcgtcg gcatcgatgt gaccgcgtcg
     6421 ggatcggatg ccggcttggt tcgcccgatg gtcgagaagt tgcagcggga gggcatcgtc
     6481 cctgagtgct accttgccga tggcggcttt accaagaacg atgacatcga atggacccac
     6541 gcccaaggcg tcgaactgct gtgcccaccg ccgaactcga agcatggcac cgatcccttt
     6601 gtggcacgca aggatgacgg cccaggcgtg cgagagtggc gcaaacgcat ggcgagcgaa
     6661 gccggccagg ccctctacaa gcgccgcgcc cagcacgaat gcatcaacgc acgactgcgg
     6721 caatgggacc tgtggcacct cgccgtccgc ggcttgaaca aagcgctcac catcctgcgg
     6781 tggttcgcgc tggctcacaa tctgctcggc gcccacaggc ttatcgctgc cgcagcggct
     6841 cgaccgggat gacgtcttga gcggatccat ggaccgcctg cccatcaccc ggtacaacta
     6901 gcggccacgc atcaatccca gggcctggcc ctctcccctt cgcctgctca tagattcaca
     6961 ggctcagtgg gggagaggga agggtgaggg ggtgcagcgg actcgatttc aacagatcac
     7021 gctcaagaca agggccatcg gcatggcgga cagcgaccag gacaatcaat tccgcgccgc
     7081 gcggctggag aagctgaagg cgctgcgcgc gctgggcgtc gatccctacc cctacgcttt
     7141 cgcccgcaac gccgaggcga aggcgctcga gggccgctat gccgcgctgc cgcccggtgc
     7201 catgacggag gatcgcgtgg cggtcgccgg ccgcatccgc gccttgcgca acagcggtct
     7261 cttcatcgac ctccacgacg agaccggcaa gatccagatc ttctcccaca aggatttctt
     7321 gcctccggcc gagctgccga tcgtcaagct gctcgatctc ggcgacatca tcggcgtcga
     7381 gggcgtagtg cgccgcacgc ccaggggcga gctcacggtg aatgccacgc atctcgccgt
     7441 gctgacgaag tcgctgctgc cgctgccgga gaaatatcac ggccttgccg acgtcgagct
     7501 gcgctaccgc cagcgctatc tcgatctcat catgaacgag gagagccgcg cgaccttgcg
     7561 ccagcgctct cgcgtcgtgg aaaccatccg ccgccttctc gcggagcgtg gtttcgtcga
     7621 ggtcgagacg ccgatgctgc agacgattcc gggcggcgcc gcggcacggc ctttcatcac
     7681 gcatcacaat gcgctcgaca tcgacctcta tctccgcatc tcgcccgagc tctatctgaa
     7741 gcgccttctg gtcggtgggc tctcggacaa ggtcttcgag ctcaaccgca acttccgcaa
     7801 cgaagggttg tcgccgcggc acaatcccga attcacgctg ctcgagctct accaggccta
     7861 tgtcgactac actgccatga tggagatcac cgagtttctc gtcacccaat cggcgcaagc
     7921 ggtgcgcggc gcgcttcgcc tctcctatgc ggggaaggag atcgatctga cgccgccctg
     7981 gccgcggcgg accatggccg agctggtgca ggagcacgcc ggcatcgatt tcctcgcgat
     8041 cggcgacgac gccggcgcgc gcagcgcggc ccggcgcatc ggcatcgagc tcgccgacac
     8101 cacgcgctgg ggccatgcgc tctatgccgt cttcgagtcg cgcgtcgaga cgctcctcgt
     8161 ccagcccatc catgtcacgg attttccgcg ggacgtgtcg ccgctgacca aggtgcaccg
     8221 cgccgatccg cgtctcaccg agcgcttcga gaccttcgtc aatggctggg agatcgccaa
     8281 cgcctattcc gagctcaacg atccccagga tcagcttgcc cgtttcgcgg cgcagatgcg
     8341 cgagcgggaa ctgggcgacg aggaggcgca gcgcctcgac gccgatttcg tcaccgctct
     8401 cgaatacggc atgccacccg cgggcggcct cggcatcggc atcgaccgtc tggtgatgct
     8461 gctcaccgac agcccctcca tccgcgacgt catcgccttc ccgacgatgc gaccgaagga
     8521 gtgaaacctc tccgcccctc gacggggcgg agaggagaga aacgatcgcg gccgctcagc
     8581 gaatatggat cgggaacacg agatcgacac ccacgggcgg cggcggcggc gcatagaccg
     8641 gtggcggggc atagacatag gccggacgcg gtgcgacata gacgcgacgg tcgcggacga
     8701 cgcgatggtt gtcgcgccga tggtcgtcgc gcgcgtcatc gcggtggtcc cggtcgtcgg
     8761 cacgcgccgg tgccgcggca aaaccgccgg ccaggccggc gagcgcgagg cttgtggcga
     8821 aagcgcccaa gatcaggcgt cggcgccctg aagacgacat ccgagcgttc atgaccatct
     8881 cctgttttgg tggcttcggc tattgctgcg gcttctgcat gctggcatgg ctcgcccggg
     8941 catggcgatg atggccgaag accgtgtcgg cattcttctt ctgctcgggc gacatcgagt
     9001 cgtagagcgt ctggaacgcg gggatcaggc gctgcaggcc ctgcacatgg gtctcggcga
     9061 ctttctgata gttgcgcaga tcgtcgaccg cgttcatctc gtcgcggtgc tgccgccggt
     9121 cggcgatcac ccccttcatt tccttgtcgt cggcgcgcat ggcgtcggcg acggcctgcc
     9181 attgctgctc ctggtccggc gtgatcttga gcgagtcatg aagattcttg atccgatcct
     9241 cgacatcggc cctggcatgg cgcggctttt tcttggtcga ggcgctcggc ggcgcggtgg
     9301 tcgagttcgc ctgagcgagt tgcaccgtgg aggacggtcc ctgatcggcg gccaccgccg
     9361 tcaaaggacc ggccacgacc atcgcgccga gcacggtcgc ggccgcgagc atgcgagctg
     9421 cgggatctag aatctgcatc atcgagagga gtcccatggt tggggttgat cgggttgtgg
     9481 gcaacggaaa gcgcgcatta tccccattcc cgagaaagat gaaacccgcc cgtgggaggg
     9541 gtagcctggc ccctcactcc gccgctactg ccgcgcggcg cagatcctcc gtcgtcgtgc
     9601 cgcgtccctc cggccgccag cccggcgcgt cgaagagata atgcagccgc tcgcgccagg
     9661 agcgcgcctg ccaaaggtcc cgcccgagcg cccgccattc gtggaaattg atgacgaagg
     9721 ggttgttcga gagcagcggc ttgacgaggc catagcggca ggggagatcc gcgcgctcct
     9781 cgatcaaggt gccgaacagg cggtcgaaga cgatgagcgt gccgccgtaa ttggcgtcga
     9841 gatattcggg gttcgcggcg tggtggacgc ggtgatgcga cggcgtgttg aagacatatt
     9901 cgagccagcc gagcttcggg atccaatcgg catgaagcca gaactgatag agcaggttca
     9961 gcgacaccac ggcgaacacc gccgggatcg ggaatcccac ccaaaccagc ggcgcgtaga
    10021 agatgccgcc gccggtcagg cggatgctcc agccgaagcg gtacgcggcc gagagattga
    10081 gctcgttcga cgagtgatgg atggcgtggg tcgtccagaa ccagcggacg cggtggctcg
    10141 cgcggtggaa ccagtagtag cagaactcga cgccgatgaa gagcagggca aaagcgagcc
    10201 ggccatccac cggcagcgtc gccaggcgat gtgccgatac ccagacgagg aacggataag
    10261 cgaagctgaa tcccaggaat gggtagacga tgtattcgcg gccgagtccg tcggcgagcg
    10321 acgccaacgc ggcgcgccag tcgtagtcgc ccttgcggcg gatggcgagc accgccgcct
    10381 cgatcaccgc cagcacgatg acagcgggca ggaaataggc gagatagccg aagagtgcgg
    10441 acatggtcga tctcctgttt gcagaccgcc cgcactatgg gaggggatcg ggccgcgggc
    10501 tcgccgcaat cggcgcgccg ctatccgaaa cctcaaccgg cctggccgat ttcgggaatc
    10561 ggcaagccaa ggggatctcc gtccaccccg ccaagcggcc gccgcacccg cgcgccgccg
    10621 ctcacgtcgg attttcgagg ctgagcacgc cgcggtcgta gtcataggcg aagagctcgg
    10681 catagcggcc ccagttgatc accgtctcga acacctcgcg cgcttcctct tcgctcagat
    10741 gatcctcgag ctcggcgatg aagcggcctt cgggtgcact gtggccgggg cgctcgtcga
    10801 gcaccttgcg gatatgcgcg gccaagggaa tgccgctctt gagccgttcg gcgaagatct
    10861 gcttgcgctt ctgcagatcg gcctcggcga agtcgcggcc caccggcgtc tcccagatgt
    10921 cgccgcgctc ggtctgcgcg aagcccagga gctgcagcgc ctcgatcagc acgaagagcg
    10981 cctcgtcctc gatatgctcg ctctcgacca gatgcggcag gtcggcgcgt cccgaatagg
    11041 gtggcgccgc cagcgcctcg agcatgccca cgagctgctg gatcgacgcg cccggcaggc
    11101 gatagccgat gccgatgggc tcgggccgct ggccgcgctt gccgccttcg cggccgggcg
    11161 cggtggtgag gagcgcatag acctcgtcga cgatctggcg gaagcgcggg ctcgcccagt
    11221 cgcgcggccg cggcaacggc accgggatct cggcgcggat atggccggga tcgctgccga
    11281 agatgatgat gcggtcggcg atctcgaccg cctcctcgat gttgtgcgag acgaggatga
    11341 tgcccttggt cggcacgcgg cgctgcagcc agagatccat gaggtcgccg cgcagcgtct
    11401 ccgccgtcag cacgtcgagc gccgagaaag gctcatcgag cagcagcacg tccggattga
    11461 cgacgagcgc ccgggcgaag cccacgcgct ggcgcatgcc gcccgacagc tccttgggat
    11521 aggcggattc gaagccgtcg aggccgatga ggtcgatcgc ctggagggcg cggtggcggc
    11581 gctcggcgcg cggcacgccc tgcgcctcga ggccgagttc gacattgccc agcacggtca
    11641 gccacggaaa gagcgcgaag gtctggaaca ccatggcgac gccgtgggcg ggaccttcga
    11701 ccgtctggcc gcgataggcg acatggccct ccgtcggcgg cgtcagcccg gcgaggacgc
    11761 gcaggaacgt cgacttgccc gagcccgact tgccgaggat ggcgacgatc tcgccttcat
    11821 gcagcgcgaa atcgacatgg tcgagcacga gacggccggc ggtctcggcg gtctggtagg
    11881 tcttgcacac cttttcggca cagatgaggc ggctgtcgtc gggcatggag gcctttccgg
    11941 tcaatcgagg cgcagccgct cttcggcgag ctgatagagc cgccgccaca gcaagcggtt
    12001 gaacagcaac acatagagcg acagtacggt gatgccgagc gcgatgcgcg ggaagtcgcc
    12061 cttctcggtc tgctgcgcga tataagcgcc gagcccggtc gccaccaggg tcgtatcgcc
    12121 ccatttcgct atttcggcga cgatgctggc attccatgaa ccgcccgagg cggtgacggc
    12181 gccggtgacg aaggccggga agatccccgg caggatcagc cgccgccacc acagccagcg
    12241 cgacacgccg agattgctgg cgacgaactg atagtcggtg gggagcgcca gcgtcccggc
    12301 gatgacgttg aagaggatgt accactgcgt gccgaggatc atgagcgggc tcagccagat
    12361 ctcgacattg aggtgaaacg cgacgatggt cgagacgacc accggaaaaa acaggttcgc
    12421 cggaaacgct gcgaggaact gcaccaccgg ctggacgcga tgcgcgagtc ccggcctgag
    12481 cccgatccag acgccgatcg gcacccacac cagcgaggcc agcgcgatca gcaccaccac
    12541 gcgcagcagc gtgatgaatc ccaggagcac gacgtgagcg acctcccgga cgtcgacctc
    12601 gctgcgcaca aagccaatga tgtagccgag cgccaccagc gtgccggcga gaagggcggt
    12661 gttccaggcg agatcgagcc agcgcggggc gaccagcgac gggcgcgagc cgcgggccgg
    12721 gcgcggcatg gcgcgctgca cgcggctcgc caggagcgcc gtgtagtcga caagccagcg
    12781 caagagatcg aagagccgcg cccgctgcag catggtgagg aaccagggcg gctcgacctc
    12841 gtcggcgccg atggcctcga ccttgaagcg ctgcgcccag gccagcagcg gccggaacag
    12901 cagctgatca tagatcagga tgaagacgag catggcgagg atggcatagc cgactgcacc
    12961 ccagtcccgc tgctcggtgg cgagcgcgat ataggagccg atgcccggca gcttcacgtc
    13021 ctggccggcg acgctgatcg cctcggacgc gaccacgaag aaccagccgc ccgacacgga
    13081 catcatcatg ttccagatga gattcggcag cgcgaagggc acctcgagct gccagaagct
    13141 ctgccaggcc gagagatgat acatgcgtgc cgcctcacgc aggtcctgcg gcaccgcgat
    13201 gaaggactgg tagaggctga aggtcatgtt ccaggcttgc gaggtgaaga tcgcgaagat
    13261 cgccgcgcat tcgtagccca ggagccggcc gggaaagagc gcgacgaagc cggtgacggt
    13321 gatcgacagg aatccaagga tcggcaccga ctgcaggatg tcgagcacgg gaatgagcag
    13381 cttctcggcg cgccggctct tggcggcaag cgccgcatag ctcaagctga aggccagcga
    13441 tgccgccagc gcgagcgcca tgcgcagcac cgtgcgcaag ccatattccg gcaggttgcg
    13501 cggatcgagc gagatcggca gcgcctcgcc cagccggtag gggacgttca tctgctggct
    13561 cgcccaggcg acgagcacga ccgcacccag caccagcggc agcgctaccc cgtcccagac
    13621 attgggccgg agccgcgacg gccaagcgac gatcggcagc ccccaacgct gttccatcgc
    13681 tcccccattg tctcgccccc gcggcgcccg acatgtagcg caatcgtccg tccgctgaaa
    13741 gcgcgacggg atcatcgacg gaggaaatcg cgaccctcca gaagcccctc tcccgcatcg
    13801 cgggaggggc aagcactgct gctatcgaga gcgctaatcc tcgagcagct ggctcatctt
    13861 gagcgcgatg cccatcgagc cctggatctt cagcttgccg gtcatatacg ccatcgtcgg
    13921 attgagcgcg ccggcaatca gccgcttgag atcgtcgaga gtgatggtga tggtcgtctc
    13981 ggcgtcgcgc gcggtgttgt cgatcgccgg cggcgtctgg gtcccgtccc aatggatgat
    14041 gccgtccgca ccgaggtcga actggatgct atggccaagc gacggcgagg tcgttgcctt
    14101 ggcgcgaatg gcggccgtgg cgtcctcgag cgacatgagc ttgctccctt tgcgagcggc
    14161 gcgctcatcc gccctcgctt cgatgatagc aacctcgcga tccctgcgca gccctgtcat
    14221 gaagcgggcg tggaagcgcc cttaagcgac gcgtgctatg aagaagggct cggggggagg
    14281 tcgccatgag cagcatcgag cgttcagtcc atgacgtaga ggcaccgcag caggcgatcg
    14341 cgccgcagac cccgggcgag ctgtggctcg cgctgcgcgc cgaagccgtt cgctccggcg
    14401 agcgcgagac ggcgctgcgc gactttctcg aactcgccgt gttgcgccat cgcgatttcg
    14461 ccgaggcgct tgcggcgctc ctcgcccaca agctggccga gcgctccatg ccgctcgagc
    14521 gcctcgagga catcgtccgc gccgccttcg ccgagcagcc gcagatcatc gccgccgcca
    14581 tcgccgacct cgccgcgatc cgcagccgcg acccggcggc agggaccccg ctcacgccct
    14641 tcctctacta caagggcttt catgcgctcg aatggcaccg cgtcggccat tggctctggc
    14701 gcggcgggcg tcgcgacctc gcgcatttcc tccagagccg cgtctcggag gtcttcgccg
    14761 tcgacatcca cccggcggtc ccggtgggaa gcggcgtctt catcgaccac ggcacgggcc
    14821 tcgtggtcgg cgagaccgcc gtgatcggcg acgacgtctc catcctccag gaggtgacgc
    14881 tgggcggcac cggcaaggag cgcggcgacc gtcatcccaa ggtgcgcgac ggcgtgctgc
    14941 tcgccgccgg cgccaaggtg ctgggcaaca tcgagatcgg ccgcggcgcc aaggtcggcg
    15001 ccggtagcgt cgtactttat gatgttccgc cctgcgcgac ggtggcgggc gtgcccgccc
    15061 gcatcgtcgg ctggtgcgac gacgggccgc cggccctggc gatggaccag agcctcccgg
    15121 agtaagttgt cagctgtcag ttatcagtca ggtgaataac tgaaaactga cgactgaaaa
    15181 ctgagcactt cccttcctac ttcaccaccc cgcaggcgat ccgcgcgccg ccgccgccca
    15241 agggcgccgg ctggtcggag tagttgtcgc cattggcatg gatgatgatg gcgtgccctt
    15301 tcacgtccgc gaccttgaga cggggcgcga cgacggtttt gccggcgtgg cccttgtcgt
    15361 tgacgacaag cacggggagg tcgccgcgat ggccgtcgct gctgtcgggg ccgagatgct
    15421 tgccggtctt gtccgggtcg aaatgaccgc ccgcggcgaa gcccgcgact tgcttgccgt
    15481 tctgctcgcc cggcccgcaa tcgccattgg cgtggacatg gaagccatgg ggcccgggcg
    15541 gcaggtcctt gagtttcggc gtgatcgaca gccctttggc ggtgtccttg aggcgcagcg
    15601 tgccgatcga ctggcccacc ccattctcgt cgatcttgtt gacggtgacc ttggccgtcg
    15661 ctgcctcggc ggccgccgca ccacataata ccagcccgca gacaagtgac agaacgatcc
    15721 tcatggtcgt ctccccccgg ttacgaattt gacgcggtcg cgatcctaag ggaatcgggc
    15781 acgggcgcca agcgcctgga gagggcatct ggcctgacgc tctgatggcg tgtgcgcggc
    15841 accgggtgac gctggtcgcg tccgactcgc cgaaatcgcc ccgcttccat ggaacggccg
    15901 atgttctcta tctccggctg cgcggcgctc gagggcctct atgtcagcgg ctatgacgaa
    15961 agcgcgctgg acggttgggc ggcacgcata gctcgctgcg cccggcgcgg ccttccatcg
    16021 cgccacgtct atatacttgc ttcgataatg acgccgaggt gcaggcgcct gccggggtcc
    16081 ggcgctcgcc cgccgcccgg gcgcagcgcc cttgccaagg cgaacgccgg agccggcgc
//
LOCUS       DASYNS010000369         3500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3525036, whole genome shotgun sequence.
ACCESSION   DASYNS010000369 DASYNS010000000
VERSION     DASYNS010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3525036"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VGP50_11525"
     CDS             complement(<1..527)
                     /locus_tag="VGP50_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HEV8028054.1"
                     /translation="MYVTVVPNRKSRPAILLRESYRDGDKIKNRTLKNLSDWPAERIE
                     QLRAVLRGDKLVPAGEGLEIMRALPHGHVAAALGTARRIGLDRLLPRGPERRRDLALA
                     LIVARLLEPSAKLATARALDETTASHSLGSTLGLGAVTAKEIYGTLDWLGKAQPAIEA
                     ALARRHLKDGTLVLY"
     gene            complement(650..1627)
                     /locus_tag="VGP50_11530"
     CDS             complement(650..1627)
                     /locus_tag="VGP50_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEV8028055.1"
                     /translation="MAIEVLKPGLATSVQDAGRPGYYNLGIPLSGALDQYALRVANLL
                     VGNDDGAAVLEAALLGPELLFRKPAIVAVAGAEAMPKVNGEPQPRNQSFAVKADDRLS
                     FGYMKLGARIYIAVAGGIDVKLVLGSRSTYGLGALGGLAGRKLAAGDVLPVGAAAQGA
                     RAGRAVSGDLIGTYPKSLEIRVLPGLYFHRLTDESAASFFADTWTVAPEADRIGYRYR
                     KGRALSFRERKQPFGAGSDPSNIVDAGYPYGSIQVPGGVEPIILHRDAVSGGGYAMIG
                     TVISADMDRIAQMQPNNTCRFVRVDMDAALQARAAYKAQLSRLRAALAG"
     gene            complement(1617..2495)
                     /locus_tag="VGP50_11535"
     CDS             complement(1617..2495)
                     /locus_tag="VGP50_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HEV8028056.1"
                     /translation="MSAVRYSFGGDEHVFVEIDEEMSLEAFFKGMAICGELQKRRIAG
                     VSEICPANASYLVRFDPDVIRPDAMLQILQGIEAEVGGADLELPTRIVEIPVLYNDPW
                     TRETLMRFRERHQDPNSADIEYAARINGYKSVDDFIAAHSGAPWFVSMVGFVAGLPFL
                     FQMVERDQQIEVPKYLRPRTDTPKLTVGHGGCFGSIYSVRGAGGYQMFGITPVPIYDP
                     AQKLPYLRDFMVFFRPGDIVKWKPISREEYDRDVKAVEAGSFNLTTRPVAFSLKSFLK
                     DPGGCSRKLVEVLHGH"
     gene            complement(2492..>3500)
                     /locus_tag="VGP50_11540"
     CDS             complement(2492..>3500)
                     /locus_tag="VGP50_11540"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424640.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV8028057.1"
                     /translation="PIVPGSAGEVQGLDAAREAARDIGYPVMIKASAGGGGRGIRVAE
                     DPAALENHLKTAQAEAAAAFGHGGLYLERFIGKARHIEVQVLGDGARVVHCFERECSL
                     QRRRQKIWEEAPSPALDGATRDALCASAVRLAESASYRGAGTLEYLYDDETGAFFFIE
                     MNTRIQVEHPVTEMITGIDLVREMILIAQGRRLSLAQGDIAMRGAAIEVRINAEDPER
                     NFMPSPGRIASLAWPGGPGVRIDTMLYPGYSVPPYYDSLLAKLIVHAEDRPSALARLA
                     GALGELEIGGIATTAGLHRRLVALDAVRSARFDTGFLERYLASGTPPTSSKSMQIRRT
                     S"
BASE COUNT          600 a         1194 c         1155 g          551 t
ORIGIN      
        1 tcgtagagca ccagcgtgcc gtctttgaga tgccggcgcg ccagcgccgc ctcgatcgcc
       61 ggctgcgcct tgcccagcca atcgagcgtg ccgtagattt ccttggccgt caccgcgccc
      121 aaccccagcg tcgagcccag cgaatggctc gccgtcgtct cgtcgagcgc gcgcgccgtc
      181 gccagcttgg ccgaaggctc aagcagccgt gccacgatca gcgccaacgc caggtcgcgt
      241 cgccgctcgg gaccccgcgg cagcagccga tcgagcccga tccgccgcgc cgttcccagc
      301 gctgccgcca cgtgtccgtg cggcagcgcg cgcatgatct ccagaccctc gcctgccggc
      361 accagcttgt caccgcgcag cacggcgcgc agttgctcga tccgctccgc cggccagtcg
      421 gacaggttct tcagcgtccg gttcttgatc ttgtcgccgt cgcgatagct ctcccgcagc
      481 aagatcgcgg gacgcgattt tcggttcggc accaccgtga cgtacatgcg aagcttgaat
      541 caccgcagca cgcctgtgtc aagcgacaat tcaccagata catgactacg tgccagaacc
      601 gatattcagc aaaatcagct acttggccga aaaccgggca aagttccgtc tagcccgcga
      661 gcgccgcccg cagccgcgag agctgcgcct tgtaggccgc gcgcgcctgc aatgccgcgt
      721 ccatatcgac gcgaacgaag cggcaggtat tgttgggctg catctgcgcg atgcggtcca
      781 tgtccgcgct gatcaccgtg ccgatcatgg catagccgcc acccgagacg gcgtcgcggt
      841 ggaggatgat cggctcgacg ccgcccggca cctggatcga gccataggga taaccggcat
      901 cgacgatgtt cgagggatcg gaacccgcgc cgaagggctg cttgcgctcg cggaaggaga
      961 gggcacgccc ttttcggtag cgatagccga tgcggtccgc ctcgggcgcc acggtccagg
     1021 tgtcggcgaa gaaggacgcg gcggattcgt cggtcagtcg gtggaagtag aggcccggca
     1081 atacgcgaat ctcgagggac ttgggatatg tgccgatgag atcgccggat acggcccggc
     1141 cggcgcgggc accttgcgcc gcagcgccga ccggcagcac gtcacccgcc gcgagcttgc
     1201 ggcccgcgag gccgccgagc gcgcccagcc cataggtcga gcggctcccc agcaccagct
     1261 tcacgtcgat gccgccggcg acggcgatgt agatgcgcgc ccccagcttc atatagccga
     1321 aggacagccg gtcgtcggcc ttcaccgcaa aggattggtt gcgtggctgg ggctcgccgt
     1381 tgaccttggg cattgcctcg gcgccggcaa cggcgacgat cgccggcttg cggaagagaa
     1441 gctccggccc gagcagcgcc gcctccagca cggcggcgcc gtcgtcgttg ccgaccagca
     1501 ggttcgcgac gcgcaaggca tattggtcaa gcgcacccga caagggaatg ccgagattgt
     1561 aatagccggg acggccggcg tcctggacac tcgtcgccag ccccggcttc aacacctcaa
     1621 tggccatgga gcacctccac cagcttgcgg ctgcagccgc cggggtcctt gaggaaggat
     1681 ttgagcgaga acgcgaccgg ccgcgtcgtc aggttgaagc ttcccgcctc gactgccttg
     1741 acgtcgcggt cgtattcctc gcgcgagatc ggtttccatt tgacgatgtc gcccggccgg
     1801 aagaaaacca tgaaatcgcg gagatagggc agcttctgcg ccgggtcgta gatcggcacc
     1861 ggggtgatgc cgaacatctg gtagccaccg gcgccgcgca cggagtagat ggagccgaag
     1921 cagccgccat ggccgaccgt cagcttcggc gtgtcggtgc gcggccgcag atatttcggc
     1981 acctcgatct gctggtcacg ctcgaccatc tggaagagga agggcagccc cgcgacgaag
     2041 cccaccatcg agacgaacca cggcgcgccg gaatgagcgg cgatgaagtc gtcgacggat
     2101 ttgtaaccgt tgatgcgcgc cgcatattcg atgtcggcgc tgttcggatc ctgatggcgc
     2161 tcgcggaagc gcatcagcgt ctcgcgcgtc cagggatcgt tgtagagcac ggggatctcg
     2221 acgatccgcg tcggcagctc gagatcggcg ccgccgactt ccgcctcgat gccctgcaag
     2281 atttggagca tcgcgtccgg gcggatcacg tccgggtcga agcggacgag ataggaggca
     2341 ttcgccgggc agatctcgga gaccccggcg atgcggcgct tctgcagctc gccgcagatc
     2401 gccatgccct tgaagaaggc ttcgagcgac atctcctcgt cgatctcgac aaagacatgc
     2461 tcatcgccgc caaaggaata ccggacagcg ctcatgaagt cctccgtatc tgcatgctct
     2521 tgctcgatgt cggcggcgtc ccgctcgcca ggtaacgctc gaggaagccc gtgtcgaagc
     2581 gcgcgctccg caccgcgtcg agcgcgacga gccgccggtg cagcccggcc gtcgtcgcga
     2641 tgccgccgat ctcgagctcg ccgagcgcgc ccgcgagccg cgccagcgcc gacgggcggt
     2701 cctcggcatg gacgatgagc ttggccagca gcgagtcgta gtaaggcggc acggaatacc
     2761 ccgggtaaag catggtatcg atgcgaacgc cgggaccgcc gggccaggcc agcgacgcga
     2821 tcctgccagg cgacggcatg aaattccgct cgggatcctc ggcattgatg cggacctcga
     2881 tggcggcgcc gcgcatggcg atgtcgccct gcgccaggct cagcctccgc ccttgggcga
     2941 tcaggatcat ctcgcgcaca agatcgatac cggtgatcat ctcggtgacg gggtgctcga
     3001 cctggatgcg ggtgttcatc tcgatgaaga agaacgcacc ggtctcgtcg tcgtaaagat
     3061 attcgagcgt accggcgccg cgataagaag cgctttcggc gagccgcacg gcagaggcgc
     3121 agagcgcgtc gcgcgtcgcg ccatcgagcg caggcgacgg cgcctcctcc cagatcttct
     3181 ggcgccgccg ctgcagcgag cattcgcgct cgaagcaatg gaccacgcgc gcgccgtcgc
     3241 cgaggacctg cacctcgata tgccgtgcct tcccgatgaa gcgctcgaga tagagcccgc
     3301 catggccgaa ggccgccgcc gcttccgcct gcgcggtctt gagatgattc tcgagcgcgg
     3361 cgggatcctc ggcgacgcgg atgccgcgcc cgccgccgcc ggcgctcgcc ttgatcatca
     3421 cgggatagcc gatgtcgcgg gcagcttcgc gcgccgcatc gaggccctgg acctccccgg
     3481 cgctgccggg gacgatcggc
//
LOCUS       DASYNS010000370         2544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2433497, whole genome shotgun sequence.
ACCESSION   DASYNS010000370 DASYNS010000000
VERSION     DASYNS010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2433497"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /gene="cobA"
                     /locus_tag="VGP50_11545"
     CDS             <1..764
                     /gene="cobA"
                     /locus_tag="VGP50_11545"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEV8028058.1"
                     /translation="DLPDFAPGSVWLVGAGPGEPGLLSLLALHALQEADVVVYDALVD
                     ARILELARPGAALDFAGKRGGKPSPQQPDISGRLIRLARAGQRVLRLKGGDPFVFGRG
                     GEEALALAAANIPFRVVPGITAAIAGLAYAGIPATHRDINSAVTFVTGHASDGEVPDG
                     LDWAALAKAAPTLVFYMALKHLDIIARRLMAAGRPADEPVAVISKATTPAQRVLVTTL
                     GRVAAETAAAGIEPPAIVAVGPAVRLRASLAWLET"
     gene            1048..1716
                     /locus_tag="VGP50_11550"
     CDS             1048..1716
                     /locus_tag="VGP50_11550"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015277772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV8028059.1"
                     /translation="MVEFAKIRKAAEKRLGAQGVAARLPPVKSPAQIKKLGDDRYLSL
                     MSLRIFRAGLKHEMVDAKWPAFETAFHGFNPKRVRAMSDEQVEALMKDAQLIRHLSKL
                     KAVHANAATICTLAEEHGSFAAWLAAWPSSEITKLWDALAKRFAQLGGNSGPTFLRMA
                     GKDTFILTPSVVAALNHWGVIEGEPKGKADRAKLQAVFNAWAEETGLKLSHLSMTLAM
                     SVAS"
     gene            1790..>2544
                     /locus_tag="VGP50_11555"
     CDS             1790..>2544
                     /locus_tag="VGP50_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV8028060.1"
                     /translation="MTDLAHIRNFAIIAHIDHGKSTLADRLIQRCGGLAEREMRDQVL
                     DSMDIERERGITIKAQTVRLAYKALNGETYQLNLMDTPGHVDFAYEVSRSLAACEGSL
                     LVVDASQGVEAQTLANVYQAIEANHEIVPVLNKIDLPAAEPQRVKQQIEDVIGIDASG
                     AIEISAKTGRNIDAVLEALVNRLPAPQGDAAAPLKALLVDSWYDAYLGVVTLVRVKDG
                     VLKAGVKIRMMVTGATHQVERVGVFTPKRVLVD"
BASE COUNT          408 a          870 c          853 g          413 t
ORIGIN      
        1 tcgatctgcc ggatttcgcg ccgggctcgg tgtggctcgt cggcgccggc ccgggcgagc
       61 cggggctctt gtcgctgctg gcactccatg cgctgcaaga agccgacgtc gtcgtctatg
      121 acgcgcttgt cgatgcgcgc atcctcgagc tggcgcgccc cggtgccgcg ctcgatttcg
      181 ccggcaagcg cggcggcaag ccgtcgccgc aacagcccga catctcgggg cggctgatcc
      241 gcctggcgcg tgcgggacag cgcgtgctgc gcctcaaggg cggcgatccc ttcgtcttcg
      301 ggcgtggcgg cgaagaggcg ctggcgctgg ccgcggccaa tatccccttc cgcgtcgtgc
      361 ccggcatcac cgcggcgatc gccggcctcg cttatgccgg cattcccgcc acccatcgcg
      421 acatcaactc ggcagtgacc ttcgtcaccg ggcatgcgag cgacggcgag gtgcccgacg
      481 ggctcgattg ggcggcgctc gccaaagccg cgccgacgct ggtgttctac atggcgctga
      541 agcatctcga catcatcgcg cgccgcctga tggcggccgg acgtcccgcc gacgagcccg
      601 tcgcggtgat cagcaaggcg acgacgccgg cgcagcgcgt gctggtgacg acgctcggcc
      661 gcgtcgccgc ggaaaccgcg gccgccggca tcgagccgcc ggccatcgtc gcggtcggcc
      721 ccgcggtgcg gctgcgcgcg agcctcgcat ggctggagac gtgaccggcc aggagcctgt
      781 ctgagtaatg gaacgggtct gcgttgcgcg gggatgggtc gcgctcagga gcggcggctc
      841 tcggcgaagc ttcgcttcgc ctgatgccgg cgcgacgcgg cagcgcccca tccccgcgca
      901 accctccggg acattggcat ttgccgctgg gcgtcgtcgc tcgtcgctta cgatgcgcat
      961 gacatcgctg cgctccttgc tcctagccca gcggcaaatg ccaatgtcgc agacccgttc
     1021 cattactcag acaggctcct gacgggaatg gtggagttcg cgaagatccg caaagcggcg
     1081 gagaagcgcc tcggcgcgca gggcgtggcg gcgcggctgc cgccggtcaa atcgcccgcg
     1141 cagatcaaga agctgggcga tgaccgctac ctctcgctca tgtcgttgcg catctttcgc
     1201 gccgggctca agcacgagat ggtcgatgcc aaatggccgg ccttcgagac ggcgttccac
     1261 ggcttcaacc cgaagcgcgt gcgcgccatg agcgacgagc aggtcgaggc gctgatgaag
     1321 gatgcgcagc tcatccgtca tctcagcaag ctcaaggcgg tgcatgccaa tgccgcgacg
     1381 atctgcacgc tggccgagga gcatggcagc ttcgccgcct ggctcgcggc ctggccctcg
     1441 agcgagatca ccaagctctg ggacgccttg gcgaagcgct tcgcgcagct cggcggcaat
     1501 tcgggaccca ccttcctgcg catggccggc aaggacacct ttatcctgac gccgtcggtc
     1561 gtcgcggcgc tcaatcactg gggcgtcatc gagggcgagc ccaaaggcaa ggccgatcgc
     1621 gccaagctgc aggcggtgtt caacgcctgg gccgaggaaa caggcctgaa gctgtcacat
     1681 ctcagcatga cgctcgccat gtcggtggcg tcatgacgcg gctgccattg cgccgcatcg
     1741 ctcacgtccc tatcttcccg acattcatgc agctcccgat cgtgttccag tgaccgatct
     1801 cgctcacatc cgcaactttg ccatcatcgc ccatatcgac cacggcaagt cgacgctggc
     1861 cgaccgcctg attcagcgct gcggcgggct tgccgagcgc gagatgcgcg atcaggtgct
     1921 cgacagcatg gacatcgagc gcgagcgcgg catcaccatc aaggcgcaga cggtgcggct
     1981 cgcctacaag gcgctgaacg gcgagaccta tcagctcaat ctcatggaca cgcccggaca
     2041 tgtcgatttc gcttatgagg tcagccgctc gctcgcggcc tgcgagggct cgctgctggt
     2101 ggtcgatgcg agccagggcg tcgaagcgca gacgctcgct aatgtctacc aggcgatcga
     2161 ggccaatcac gagatcgttc ccgtgctcaa caagatcgac ttgccggcgg ccgagccgca
     2221 gcgcgtcaag cagcagatcg aggacgtgat cggcatcgac gcctcgggcg cgatcgagat
     2281 ctccgccaag accggacgca acatcgatgc ggtgctggaa gccctggtca atcgcctgcc
     2341 ggcgcctcag ggcgatgccg cggcgcctct gaaggcgctg ctcgtcgaca gctggtacga
     2401 cgcgtatctc ggcgtcgtca ccctcgtgcg cgtcaaggac ggggtgctca aggcaggcgt
     2461 caagatccgc atgatggtga ccggcgcgac gcaccaggtc gagcgggtcg gcgtcttcac
     2521 gccgaagcgg gtgctcgtcg acga
//
LOCUS       DASYNS010000371         6408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_871880, whole genome shotgun sequence.
ACCESSION   DASYNS010000371 DASYNS010000000
VERSION     DASYNS010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871880"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..567)
                     /locus_tag="VGP50_11560"
     CDS             complement(202..567)
                     /locus_tag="VGP50_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028061.1"
                     /translation="MRFRVLVAGVALAACSGGSQLARDLHSAESSCDKDWPTKSQFVA
                     CLDSKDRQVWAKEEPATLDLYDRFARRRDALARDFDQKEIDEHQYLERLHKLKHETRA
                     QMAARRGQNSEPDQAPAQP"
     gene            complement(613..1014)
                     /locus_tag="VGP50_11565"
     CDS             complement(613..1014)
                     /locus_tag="VGP50_11565"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV8028062.1"
                     /translation="MASIRREILTRAHPEEVWAAIRDIGALHTRLVPGFVVDTRLEPG
                     ARIVTFGNRMVVREPIVDINDRDRRLVWSAIGGRTTHYNASVQVFAGADGGARVVWIA
                     DVLPDAVTPDIQAAIEQGMAVMKKTLDRLAE"
     gene            1130..1966
                     /locus_tag="VGP50_11570"
     CDS             1130..1966
                     /locus_tag="VGP50_11570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8028063.1"
                     /translation="MDDPEAGSEPSIERWRAGSIADFLAFWLATPVLAREHQAVFDHY
                     YRSYKRHFGPYLRHWYARQTAELTALIRAGRHASVLEVGCGCGTESLWAALNGARVTA
                     IDISRDLLAVAQQRQAWMEQCTGRTLDCRFLDRSILETDDLAPFDIVYMEQAFHHLEP
                     RAEVVRRTARLVSPGGRLIIAEANGWNPLMQIKLFALRGVKTVITREGRSWGHERVTV
                     PAALIRQFGEHGLERESLDYYRALPNARVADTLLWLDRRLPAALRPLFTHFNLVLRKS
                     ES"
     gene            complement(1989..3698)
                     /locus_tag="VGP50_11575"
     CDS             complement(1989..3698)
                     /locus_tag="VGP50_11575"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV8028064.1"
                     /translation="MRTHWGLFIVGVILILAGALAAHLTQTSRGVRIEDVRFTGTNGV
                     QMSALLYVPPGATAKTPVPGILAVHGYINSRETQDGFAIEFARRGYVVLALDETGHGY
                     SAPPAFANGFGGPDGLKYLRSLDIVDKENIGLEGHSMGGWTVLAAAAAFPNDYKAIVL
                     EGSSTGAPFAAEGTPQYPRNLALVYSRYDEFSELMWQVPTGQMANESKKLLKLFGVAD
                     PVEPGKLYGAIESGTARVLYVPAITHPMDHISPEAIGYSLDWFQRNLTGGTPLPAADQ
                     VWYGKEIGTLVAFAGFIVLLLGTFDVLLTQRYFAELAQVPVALNEARSWRWWLTLALT
                     AFVPPATFFLFFQWGAQWLKASALLPEGITNQIMVWAVLNGVITLVLALVFGKRPAFS
                     GRWLPAVLAALLTVAVGYAALAVVDFVFKVDFRFWIVALRPMSRTQFVYFWVYLLPFV
                     AFFLVALRTLHAELGVKSDGAARQYLSNVAALALGFLVLLAAEYGTLFVTGHLLTPSE
                     PLNTIVAIQFLPLLAIVGVITTFTYRRTNSYVPGALISALFVTWYIVAGQAMQSPVWP
                     GAL"
     gene            complement(3856..5019)
                     /locus_tag="VGP50_11580"
     CDS             complement(3856..5019)
                     /locus_tag="VGP50_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEV8028065.1"
                     /translation="MSREPRIYELHVADEAIADLVQRLARTRFPDQAPGAAWAYGTDV
                     GYMQALVAHWRERIDWRKEEARLNQFPQYRVALDDVDLHFLHVPGKGPAPLPLLLSHG
                     WPGSVFEFLELIPRLTDPARFGGDPADAFTVVAPSLPGYGLSFAPGQKRFNIAAIADC
                     FSELMTGVLGYRRFAAQGGDWGGAITARLGQAHAERMIGIHMNLLPLRRDLKMVAEAT
                     AEEQRYYGELAHWRRDETGYQWIQGTRPQTLAFALTDSPSGLAAWIVEKFRAWSDCVG
                     DLDSIFSKDKILANIGFYWFTGAIGSSFWPYYARMHEPWPIPEGATIDVPTGYAEFPR
                     EILRPPRSLAAHVFKDIRRWSVMKKGGHFAALEQPDALAFELREFFRPLRGAR"
     gene            complement(5184..5309)
                     /locus_tag="VGP50_11585"
     CDS             complement(5184..5309)
                     /locus_tag="VGP50_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028066.1"
                     /translation="MRVAIARVLLSVLLLAGAGAALSACHHTAEGAKEDIHQDTK"
     gene            5552..>6408
                     /locus_tag="VGP50_11590"
     CDS             5552..>6408
                     /locus_tag="VGP50_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV8028067.1"
                     /translation="MLRRFGVAPRTLAAGSLVVRSPIDGRETARLATTSPGDVARVIG
                     EAEAAFLAWRDVPAPKRGELVRLLGEGLRAEKAALGRLVSIEAGKILQEGQGEVQEMI
                     DICDFALGLSRQLYGLTIATERPGHKMMETWHPLGVVGIISAFNFPVAVWSWNAALAL
                     VCGDSLVWKPSEKTPLTALACQAMLERAAARHGGVPAGLSAVIVGGAEIGEALVADRR
                     VALVSATGSTRMGRAVAPRIAERFGRAILELGGNNGMIVAPSADLELALRAILFAAAG
                     TAGQRCTTLR"
BASE COUNT         1083 a         2146 c         2139 g         1040 t
ORIGIN      
        1 cggacatccg gatcatcatc caccaccagt acgacagggc cgctcggcat cgagctacac
       61 acgatattgc ttccaggtga cctgccaaca ccgacaaaac ggtgtcgaga ccgtcttcag
      121 cctgcagcgg cggccacccg agcgactgca actggcgggt ggtgcgcatc ggctcgcctg
      181 aaatcggcca tcggtcgacc gtcagggttg cgccggcgcc tgatcaggct ccgagttttg
      241 gcctcttcga gcggccatct gcgcgcgggt ctcgtgtttg agcttgtgca ggcgctcgag
      301 atattggtgc tcgtcgatct ccttctgatc gaagtcgcgg gcgagcgcgt cgcgccgccg
      361 ggcgaagcgg tcgtaaagat cgagcgtggc gggctcttct tttgcccaga cctgacggtc
      421 cttcgagtcg aggcaggcga cgaactgcga tttggtcggc cagtccttgt cgcaggagga
      481 ttcggccgag tgcagatcgc gggcgagctg gctgccgccg ctgcacgccg cgagcgcaac
      541 gccggcgacg aggacacgga aacgcatgga tcgctccagg tcttttccaa gagcgaacgc
      601 tggcgacgcg ccctattcgg cgaggcgatc caaggtcttt ttcatgaccg ccattccctg
      661 ctcgatcgcc gcctggatgt cgggagtgac cgcatccggc aggacatcgg cgatccacac
      721 gacgcgcgcg ccaccgtccg cgccggcgaa cacctgaacc gaggcgttgt agtgcgtcgt
      781 ccgcccgccg atcgccgacc agacgagccg gcggtcccgg tcgttgatgt cgacgatcgg
      841 ctcgcgcacc accatgcggt tgccgaaggt gacgatgcgg gccccgggct cgagccgcgt
      901 atcgacgacg aagccgggca cgagccgcgt atgcagcgcg ccgatgtcgc gaatggccgc
      961 ccagacctcc tcggggtgcg ctctggtcag gatctcgcgg cgaatcgaag ccatggcgct
     1021 gctctccatc ttgtgaatgc atctctgatc gaggatggcc tcgctccccg ccctgcgctt
     1081 ggggaaaatt gctatctgta ggctccgagc gcgtcgaggg aagtcgacca tggacgatcc
     1141 ggaagccggc agcgagccgt cgatcgagcg ctggcgggcg ggctcgattg cggatttcct
     1201 ggcattctgg ctggcgaccc cggtgctcgc gcgcgagcac caggcggtct tcgatcacta
     1261 ctatcgcagc tacaagcgtc atttcggtcc ctatctccgg cattggtatg cgcggcagac
     1321 ggcggagctc accgccctca tcagggcagg gcggcacgcg agcgtcctcg aggtcggctg
     1381 cggctgcggc accgagtcgc tgtgggcggc gctcaacggc gcccgcgtca ccgcgatcga
     1441 catctcgcgc gacctgctcg ccgtcgcgca gcagcggcag gcgtggatgg agcagtgcac
     1501 gggccggacg ctcgactgcc gctttctcga ccgctcgatc ctcgagaccg acgacctggc
     1561 gcccttcgac atcgtttaca tggagcaggc cttccaccat ctcgagccgc gcgccgaggt
     1621 cgtccgccgg accgcgcgcc tcgtgtcacc gggcgggcgg ctcatcatcg ccgaggcgaa
     1681 tggatggaat ccgctgatgc agatcaagtt gttcgcgctg cgcggcgtca agaccgtcat
     1741 cacgcgcgag ggtcgctcct ggggccatga gcgcgtcacc gtgcccgcgg cgctcatccg
     1801 gcagttcggc gagcacggcc tcgagcgcga gtccctggac tattaccggg cgctgcccaa
     1861 tgcccgcgtc gccgacacgc tcttatggct ggaccgccgg ctgcccgcag cgttgcggcc
     1921 gctcttcacg catttcaatc tcgtgctgcg caagagcgag agctaggagc gatcgtcgcg
     1981 cgctcgcgct acagcgcgcc gggccagacc ggcgactgca tcgcttgccc ggcaacgatg
     2041 taccaagtga cgaagagcgc ggaaatgagc gcccccggta cgtagctgtt ggtgcggcga
     2101 taggtgaagg tggtgatgac gccgacgatc gccagcagcg gcaggaattg gatcgcgacg
     2161 atcgtgttga gcggctcgga cggggtgagc aagtgaccgg tgacgaagag cgtcccgtat
     2221 tcggccgcga ggagcaccag gaatcccaac gccagcgcgg cgacgttact gagatactgt
     2281 cgcgccgcgc cgtcgctttt cacgccaagc tcggcatgaa gcgtgcgcaa ggcgacgagg
     2341 aagaaggcga cgaagggaag cagatagacc cagaaataga cgaattgcgt ccggctcatc
     2401 ggcctgagcg cgacgatcca gaagcggaaa tcgaccttga agacgaagtc cacgaccgcg
     2461 agcgccgcat agccgacggc gacggtgagg agggcggcca gaaccgccgg cagccatcgc
     2521 ccgctgaagg ccggccgctt gccgaagacg agcgcgagca ccagggtgat gacgccgttg
     2581 agcaccgccc agaccatgat ctgattggtg atgccctccg gcaacagggc ggaagccttc
     2641 agccattggg cgccccactg gaagaacagg aagaaggtcg cgggcggaac gaaggcggtg
     2701 agggcgagcg tcagccacca gcgccagctt cgtgcctcgt tgagcgccac gggcacttgc
     2761 gcgagctcgg caaaatagcg ctgcgtcaac agcacatcga aggtgccgag gagcagaacg
     2821 atgaaaccgg cgaacgccac cagcgtgccg atctccttcc cgtaccagac ctggtcggcg
     2881 gcgggaagcg gcgtgccgcc cgtcaaattg cgctggaacc agtcgaggct gtagccgatg
     2941 gcctcgggcg agatgtgatc catgggatgg gtgatcgccg gcacgtacag cacccgcgcc
     3001 gtgccgctct cgatcgcacc atagagcttg ccgggctcga ccggatcggc cacgccgaag
     3061 agcttcaaca gcttcttgct ctcattcgcc atttgcccgg tcggcacctg ccacatcagt
     3121 tccgagaatt cgtcatatct gctgtagacc agcgccaggt tgcgcgggta ttgcggcgtg
     3181 ccctcggccg cgaagggcgc gcccgtgctc gatccttcaa ggacgatggc cttgtaatcg
     3241 ttgggaaacg cggcggcggc ggcgagcacc gtccagccgc ccatgctgtg tccctcgagg
     3301 ccgatattct ctttgtcgac gatgtcgagg ctgcgcaggt atttgagccc gtccggcccg
     3361 ccgaagccgt tggcaaaggc cggcggcgcg ctgtagccgt ggccggtctc gtcgagcgcc
     3421 agcacgacat agccgcgccg cgcgaactcg atcgcaaacc cgtcctgggt ctcgcgggaa
     3481 ttgatgtagc catgcaccgc caggatcccg ggcaccggcg tcttcgccgt ggcgcccggc
     3541 gggacataga ggagggcgct catctgcacc ccgttcgtgc cggtgaaccg cacatcctcg
     3601 attcggacgc cgcggctggt ctgggtcaga tgcgccgcaa gcgcgccggc gagaatcaag
     3661 atcacgccga cgatgaacaa gccccaatgt gtcctcaagg tcgtctccct tacccgatct
     3721 ttttctattc tcgggtcagg atcatcgacc tttcctcggc tgtcaattcc ttggctgtca
     3781 atcgccgcag cccgcagccg ctcgtcatga cgaccaacgg caccccgccc gcaacttctt
     3841 gcgtggtcga gcggctcagc gcgcgccgcg cagcggccgg aagaactcgc gcagctcgaa
     3901 ggccagtgcg tccggctgct ccagggccgc aaaatgtccg cccttcttca tcacgctcca
     3961 gcgccggatg tccttgaaga cgtgcgccgc gagcgagcgc ggcgggcgga ggatctcgcg
     4021 cgggaactcg gcatagcccg tggggacgtc gatggtggcg ccttcgggga tcggccaggg
     4081 ctcatgcatc cgcgcgtaat agggccagaa cgaggagccg atcgcgccgg tgaaccagta
     4141 aaagccgata ttcgcgagga tcttgtcctt gctgaagatg ctgtcgagat cgccgacgca
     4201 atcagaccag gcgcggaact tctcgacgat ccacgcggcg agcccggaag gtgaatccgt
     4261 gagcgcgaag gcaagggtct gcggccgcgt gccctggatc cactgatagc cggtctcgtc
     4321 tctgcgccaa tgcgcgagtt caccgtagta gcgctgttct tccgccgtcg cctcggcgac
     4381 cattttcaga tcgcggcgca gcggcagcag attcatgtga atgccgatca tgcgctcggc
     4441 atgagcctgc ccgagccgcg cggtgatggc gccgccccag tcgccgcctt gcgcggcaaa
     4501 gcggcgatag ccgagcaccc cggtcatcag ttccgagaag cagtcggcga tcgccgcgat
     4561 gttgaagcgc ttctgccccg gcgcgaagga aaggccgtag cccggaagcg acggcgccac
     4621 caccgtgaag gcgtcggcgg gatcgccgcc gaaccgtgcc ggatcggtga gacgcggaat
     4681 gagctcgagg aactcgaaca ccgagcccgg ccagccatgc gacagcagca acggcagcgg
     4741 tgccggccct ttccccggca catggaggaa atgcagatcg acgtcgtcga gcgccacgcg
     4801 gtattgcgga aactggttga ggcgcgcctc ttctttgcgc caatcgatgc gctcgcgcca
     4861 atgagcgacg agcgcctgca tatagccgac atccgtgcca taggcccagg cagcgccggg
     4921 cgcctggtcg gggaagcgcg tgcgcgcgag gcgctgcacc agatcggcga tcgcttcgtc
     4981 cgccacatgg agctcataga tcctgggttc gcggctcatt gtcatcctcc gtccgatccc
     5041 gctcaactca gcagagttgc acagaacgcg atacgtatac acatcccctc tccccggagg
     5101 ggagaggggg atgtgcggcg atgcgtgacg cgcataaaaa aagccccgcc gaagcggggc
     5161 tcgaggatgc aatcaccctg ttgctacttg gtgtcctggt gaatgtcctc cttcgcgcct
     5221 tcggcggtgt ggtggcacgc cgacaatgcg gccccggcgc cggcgaggag gaggacgctg
     5281 agcaaaactc gagcaattgc tacgcgcatg gatgcactcc ctgttggtgc tctcgatctc
     5341 caacacgctc gccattgccg tggttccgcg gtgccgtggg cagccgccgg tcgagagcgc
     5401 cgctctcaat gaacgcggcg cggcccgtcg cgccgctgct gccccggcgc tagaatgcgc
     5461 aggatctctc tcgactccgc gaatggcagg agcttcgccc gtgaccacga ccggaacagc
     5521 gacaattcct tatgccggcg acgtcgccga tctgctgcgc cgcttcggcg tcgctccgcg
     5581 cacgctcgcc gccggcagcc tcgtcgtccg ctcgccgatc gacggccgcg agaccgcccg
     5641 cctcgcgacc acctcgcccg gcgatgtcgc gcgtgtcatc ggtgaggccg aagccgcgtt
     5701 cctcgcctgg cgcgacgtgc cggcaccgaa gcgcggcgag ctcgtgcgcc tcctcggcga
     5761 aggcttgcgc gccgagaagg cggcgctggg ccggctcgtt tccatcgagg ccggcaagat
     5821 cctgcaggaa gggcaaggcg aagtgcagga gatgatcgac atctgcgact tcgcgctcgg
     5881 cctctcgcgt cagctttatg gcctgaccat cgccaccgag cgtcccggcc acaagatgat
     5941 ggagacctgg catccgctcg gcgtcgtcgg catcatcagc gccttcaact ttcccgtcgc
     6001 ggtgtggtcg tggaacgcgg cgctggcgct cgtctgcggc gacagcctcg tgtggaagcc
     6061 ctcggagaag acgccgctca cggcgctcgc ctgccaggcc atgctcgagc gcgcggccgc
     6121 ccggcatggc ggcgtgcccg cgggcctctc ggcggtgatc gtcggcggcg ccgagatcgg
     6181 cgaggcgctg gtcgccgacc gccgcgtggc gctggtgagc gccaccggct cgacgcggat
     6241 gggccgcgcc gtggcaccgc gcatcgccga gcgcttcggc cgggcgatcc tcgagctcgg
     6301 cggcaacaac ggcatgatcg tggcaccgtc ggccgatctc gagctcgcgc tccgtgccat
     6361 tctcttcgcc gccgccggca cggccggcca gcgctgcacg acgctgcg
//
LOCUS       DASYNS010000372         4244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3993817, whole genome shotgun sequence.
ACCESSION   DASYNS010000372 DASYNS010000000
VERSION     DASYNS010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3993817"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..1279)
                     /locus_tag="VGP50_11595"
     CDS             complement(293..1279)
                     /locus_tag="VGP50_11595"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEV8028068.1"
                     /translation="MKLRLVVLAALGVLLGALLVARQDLHALAHALRLAGWTALLAIT
                     LIHLVPMALCGLAWRAQLAQPPASIARYAWFRWMRETGGVTPMGGELLGVRVMALSGI
                     KVGLAIATTVVDVTIEMSAQIVFTLLGLAILVVERPHSALVAWTLAGLVVTLPLLAGL
                     VLAPRLGLFRLLERFADTLAAGRGWHGFGAGDIPAHIHAIYGRHRRILAALAIHLAAW
                     LVGIAEAGAALALMGVPLRLDSLLVLESLSYALRSAAFFVPAAAGVQEGGYVLLGALL
                     GIGPEFALALSLLKRARELLLSGLGLLAWQAAEGGRLWRRLRTPVEAQAGED"
     gene            complement(1276..2148)
                     /gene="hpnK"
                     /locus_tag="VGP50_11600"
     CDS             complement(1276..2148)
                     /gene="hpnK"
                     /locus_tag="VGP50_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HEV8028069.1"
                     /translation="MNGRRLIVTADDFGLAVPVNEAVEQAHRFGILSAASLMVTGSAA
                     KDAVARARRLPKLGVGLHLTLVDGRPALPPEAVSELVAADGRFLADPWRVGVKIYCSA
                     KARRQAEAEIRAQLLAFWRTGLTLDHVNGHHHFHLHPTVQEILLKLAPEFDIRAIRVP
                     REPPLLAWRATGQGLMRRLGTWLFQARMTARMKNRLRAAGIETNDWLLGLGDTGHMRV
                     ERLRGYLAHLPDGISELYFHPAVERWAGHGAWPSGYESRGEFEALTDPDVMALTRGSG
                     IEPVCFARLGERAA"
     gene            complement(2145..3314)
                     /gene="hpnI"
                     /locus_tag="VGP50_11605"
     CDS             complement(2145..3314)
                     /gene="hpnI"
                     /locus_tag="VGP50_11605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HEV8028070.1"
                     /translation="MGAMMAAALGATGVGELAGGVIAAASIVYLLLALWRVMRYRLPA
                     GPSPGGLPPVTVLKPICGAEPGLYDCLRSFCEQDHPRTQLIFGLRDPADPAIPVIRRV
                     IKKFPALDLALVIDPEVHGTNLKVSNLINMHRAAKHDIIVVSDSDTRIGRHDLSRVAA
                     PFDDERTGAVTCLYKAAPLGGMASRLGALFINDWVLSSAIVDAGLRDVAYCFGPLTAI
                     RREALSQIGGFDRLASQLADDFLLGRLIAAKGWKVRLSECIVDTMVAESFGSLLRHEL
                     RWARTVKTMRPAEHFLSVVTEPLPLLLALLLPNASLWGIAALALSMSLRAALHVVVQR
                     RFRPGEALTPWLLPLRECLCFGVWAWSFLGRQVHWRQRRFAIGAGGRLIAVEQRA"
     gene            3507..4106
                     /locus_tag="VGP50_11610"
     CDS             3507..4106
                     /locus_tag="VGP50_11610"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8028071.1"
                     /translation="MRKVNQERFEERQRHIREAALACFRRSGFHQASMADICAEAQMS
                     AGNLYRYYDSKEAIIAAICEHDRQEVTQRFESMQGKPDLFAAMLEMSGEAMDASAQLE
                     KSKFGVEVLSEAMRNERVAEIVRRHHQALLEVCANALAQAQAMKFIDPTLDPKLAATV
                     LIAAGEGLGFKLALHRDLDAKQCAEIFKTLILRFLRPQS"
     gene            complement(4152..>4244)
                     /locus_tag="VGP50_11615"
     CDS             complement(4152..>4244)
                     /locus_tag="VGP50_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HEV8028072.1"
                     /translation="LGTWQGIYLFEHRRAPHRREVVLQLIGESA"
BASE COUNT          776 a         1450 c         1446 g          572 t
ORIGIN      
        1 cgctgaagcg cgtcgtgcaa attgcgacaa gcgcgccatc aagcccctct cccgcaccgc
       61 gggagaggga ggggcccact gcgaagcttc agcgaagcag tgggagggtg aggggccggc
      121 gccgacggaa gtttagaccg agcgaattgc gccgccccct caccctgccc tctcccccac
      181 ttcgtggggg cgaggggaaa aaataaggtg agcgcttcga atgtgtgaat cccctagcgc
      241 gatgcgggag agcggatgtt tggcttggtt tggacgcgaa ggccgacgcg tcctaatcct
      301 ctcccgcttg cgcttcgacc ggcgtgcgga ggcggcgcca gaggcggccg ccttccgccg
      361 cctgccacgc aagcaatccg agaccgctca gcagcagctc tcgcgcgcgc ttcaggagtg
      421 acagggcgag cgcgaattcg gggccgatgc cgaggagcgc gccgagcagc acatagccgc
      481 cttcctgcac gcccgccgcg gccggcacga agaaggcggc gctgcgcaag gcatagctca
      541 ggctctccaa aaccagcaga gaatcgaggc ggagcggcac gcccatcagc gccagcgccg
      601 cgcctgcttc ggcaatgccg acgagccagg ccgccagatg aatcgcaagc gcggcgagaa
      661 tgcgccgatg ccggccatag atcgcgtgga tatgcgcggg gatgtcgccg gcgccgaagc
      721 cgtgccagcc gcggccggcg gcgagcgtat cggcgaaacg ctcgagcagg cggaaaagcc
      781 ccaaacgcgg cgccagcacc aggcccgcca gcagcggcag cgtcaccacc aggccggcca
      841 gcgtccaggc gacgagcgcg ctgtgcggac gctcgaccac caggatcgcg aggccgagca
      901 gcgtgaaaac gatctgcgcg ctcatctcga tcgtgacatc gaccaccgtc gtcgcgatgg
      961 cgaggccgac cttgatgccg gacagtgcca tcacgcgcac gcccaggagc tcgccgccca
     1021 tcggtgtgac gccgccggtc tcgcgcatcc agcggaacca cgcataacgc gcgatgctgg
     1081 ccggcggctg cgcaagctga gcgcgccagg cgagcccgca cagcgccatc ggcacgagat
     1141 ggatgagcgt gatcgcgagg agcgcggtcc agccggcgag gcgcagcgca tgggcgagcg
     1201 catgcagatc ctgccgcgcc accagcagcg cgccgagcag cacgcccagc gcggccagca
     1261 cgacaaggcg cagcttcatg ccgcccgctc cccgagccgc gcgaagcaca cgggctcgat
     1321 gccgctgccg cgcgtcagag ccatcacatc gggatccgtc aacgcctcga actcgccgcg
     1381 gctttcataa cccgagggcc aggccccgtg gcccgcccag cgctcgaccg ccgggtggaa
     1441 gtagagctcg gagatgccgt cgggcagatg ggcgagatag cccctgagcc gctccacacg
     1501 catgtgcccg gtgtcgccga gaccgagaag ccagtcattg gtctcgatgc ccgccgcgcg
     1561 cagccggttc ttcatccgcg ccgtcatccg cgcctggaag agccaggtgc cgagccgccg
     1621 catcagcccc tggccggtgg cgcgccaggc gaggagcggc ggctcgcgcg gcacgcggat
     1681 ggcgcggatg tcgaattccg gcgcgagctt cagcaggatc tcctgcaccg tcggatgcag
     1741 atggaaatga tgatggccgt tgacgtgatc gagcgtgagg ccggtgcgcc agaacgccag
     1801 gagctgggcg cggatctcgg cctcggcctg ccgccgcgcc ttggcgctgc aatagatctt
     1861 gacgccgacg cgccagggat cggcgaggaa gcgcccgtcg gccgccacca gctcggagac
     1921 cgcctcgggc ggcagggcgg gccggccgtc gacgagcgtc aaatgcaggc cgacgccgag
     1981 cttcggcagc cgccgcgccc gcgccaccgc gtccttcgcg gccgaccccg tcaccatgag
     2041 gctcgccgcg ctcaggatgc cgaagcgatg cgcctgctcc acggcttcgt tgaccgggac
     2101 cgcgaggccg aaatcgtcgg cggtgacgat gaggcggcgt ccgttcacgc gcgctgctcc
     2161 acggcgatga ggcgtccgcc cgcgccgatg gcgaagcgac gttgccgcca atgcacctgg
     2221 cggccgagga agctccaggc ccagacgccg aagcacaggc attcgcgcag cggcagcagc
     2281 catggcgtga gcgcctcgcc ggggcggaag cgccgctgca ccacgacatg gagcgccgcg
     2341 cgcaagctca tcgacagcgc gagcgccgcg atgccccaaa gcgaggcgtt gggcagcagc
     2401 agcgccagca gcagcggcag cggctcggtc accaccgaga ggaaatgctc ggccggccgc
     2461 atcgttttca ccgtccgcgc ccagcgcagc tcatggcgaa gcagcgagcc gaagctctcc
     2521 gcgaccatcg tgtcgacgat gcattcggaa agccgcactt tccagccctt ggccgcgatc
     2581 agccggccca gcaggaaatc gtcggcgagc tgcgaggcga gccggtcgaa cccgccgatc
     2641 tggctgagcg cctcgcgccg gatcgcggtc agcggaccga agcaataggc gacgtcgcgc
     2701 aacccggcat cgacgatcgc cgaggacagg acccagtcgt tgatgaaaag cgcgcccagc
     2761 cgggaggcca tgccgccgag cggcgccgcc ttgtaaaggc aggtgaccgc ccccgtccgc
     2821 tcatcgtcga agggcgccgc aacgcgggag agatcatggc ggccgatccg cgtgtcgctg
     2881 tcggaaacga cgatgatgtc gtgcttcgcc gcccggtgca tgttgatgag gttgctgacc
     2941 ttgaggttgg tgccgtggac ctccgggtcg atcacaagcg cgagatcgag ggccgggaat
     3001 ttcttgatca cccgcctgat caccggtatg gcaggatctg cgggatcgcg caggccgaag
     3061 atcaactggg tccgggggtg gtcctgctcg cagaaggagc gcaagcagtc atagagcccc
     3121 ggctcggcgc cgcagatcgg cttgagcacg gtcaccggcg gaagcccgcc ggggctcggc
     3181 ccggctggaa ggcgataccg catcacgcgc caaagcgcga gtaggagata gacgatcgat
     3241 gctgcagcaa tcacccctcc cgccagctcc ccgacgccag tggcgccgag cgcagcggcc
     3301 atcatcgcgc ccatcgattg ctccctccct ttttgccacg cgctatggga ttgagagaac
     3361 gatcgatcat tcttatatta gaaattgtgc gtcgtcaatc accggatgag cgcttgcaga
     3421 gcggccatgc gtccctattt cagagttgag agcgaccgtt cgctcttgta cataactaac
     3481 ctgagttggg ggtgaacgcg ttaaccatgc gcaaggtcaa ccaggagcgt ttcgaggagc
     3541 ggcagcgcca tattcgcgag gcggcgctcg cctgtttccg gcggtcgggc ttccaccagg
     3601 cgagcatggc ggacatttgc gccgaggcgc agatgagcgc cggcaatctc tatcgctact
     3661 acgacagcaa ggaagcgatc atcgcggcca tctgcgagca tgaccgccag gaagtgacgc
     3721 agcgcttcga gtcgatgcaa ggcaagcccg atctcttcgc ggcgatgctc gagatgagcg
     3781 gcgaggccat ggacgccagc gcccagctcg agaagagcaa gttcggcgtc gaggtgctgt
     3841 cggaggccat gcgcaacgag cgcgtcgccg agatcgtgcg ccgccaccac caggcgctgc
     3901 tcgaggtctg cgccaacgcc ctggcgcaag cgcaggcgat gaagttcatc gaccccacgc
     3961 tcgatccgaa gctggccgcc acggtgctca tcgcggccgg cgaaggtctc ggcttcaagc
     4021 tcgcgctcca ccgcgacctc gacgccaagc aatgcgccga gatcttcaag accttgatcc
     4081 tgcggttttt gcggccgcag agctagcgcg acgcgaagcg tcgtcgctgg cgccgccgat
     4141 cggcgaaagc gctaagcgct ttcgccgatc aactgcagca ccacctcacg ccgatgtggc
     4201 gcgcggcggt gctcgaaaag gtagatgccc tgccacgtgc cgag
//
LOCUS       DASYNS010000373         3943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_93441, whole genome shotgun sequence.
ACCESSION   DASYNS010000373 DASYNS010000000
VERSION     DASYNS010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93441"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VGP50_11620"
     CDS             complement(<1..932)
                     /locus_tag="VGP50_11620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV8028073.1"
                     /translation="MKLLWFHLMPYARLPEDFRQRHNSVWVDLDRSLFDPVYAHRLYN
                     EYIDELEYAAALGFDAVCVNEHHANAYGLMPSPNLIAAMLARRGGDAAICVLGNSLAL
                     YNPPTRVAEEMAMLDCISGGRLIAGFPVGTPMDTCYAYGQNPSMLRAKYQEAHDLVLQ
                     AWTRPETFAFNGRFNQLRYVNIWPQPLQKPHPPIWIPGGGSLETWQWCAEMDYVYCYL
                     SYFGYMAGRETMMGFWREMERLGKDRNPYRAGFLQFVGVAESRAEAEKLYKEPAEYFY
                     GRCLHVDSRYASPPGYASEATQRARITSQVTLAA"
     gene            1119..1931
                     /locus_tag="VGP50_11625"
     CDS             1119..1931
                     /locus_tag="VGP50_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028074.1"
                     /translation="MKSMLAALAATVAALTTPVAAQQAPPPPPGPPPAPPPAAYPPGP
                     PPAAYPPVPTIMWLPGAYIRADAGYAFTFDTDFRDVNFAQTLGDGVRIKGDSGNSPFY
                     EAGLGYRFTRFFRADLTANYLPSLKFSGTDNIGLGTVNNAQIHSEAAFANAYFDLPAM
                     LTIFGPFVQPYFDVGVGAARDHVGSFSSTFPGIAGTISPHTTTRPAVAFGLGTAISLG
                     RNSVLDLAYRFMDLGELRTGSAVTAGGVTTGISPIHSELYANVVTLGIRFAF"
     gene            2024..3712
                     /locus_tag="VGP50_11630"
     CDS             2024..3712
                     /locus_tag="VGP50_11630"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV8028075.1"
                     /translation="MQYDLKIAGGSIIDGTGKARFAGDVGISAGKVVALGQAPGDAVE
                     TIDATGRVVAPGFVDIHTHYDAQILWDQRLTISPWHGVTTVVMGNCGFGIAPTRQVDR
                     DRIVRTLEKVEGMSVAALEAGLGAEWGFATFPQYLDAVERKGPAINVAALIGHTPVRL
                     FAMGEAATERAATAEEVAAMRGIVRDGLDAGALGFATSKAITHVGYGGKPVPSRAAAF
                     SEVLALAGALREAGKGVIQATIGPGLQFDEFAALARESGAHVSWTALLAGRPRQGVDH
                     RVQLARSLELARQNYAVFPQVTPRPLNFELQLKEPFVFESMSLFRPISAADFAGKKRL
                     YADADFRKAFRDKMDRAPGEFVLGFRSTMISEYAPDPSLEERRLVDVAAERGVHPVDL
                     MLDLGLATDLDARFRMAVANNNEDDVAELLLDPHMVMGLSDAGAHASQLCDACQATYL
                     LGRWVREKQALSLEQAIRMLTSRPAEVFGIADRGRLAAGWPADIVVFDPATVGAGKLR
                     RVWDFPGGANRLVSDASGIDAVIVNGTVLRQNGRDRVAPDGKLPGKLLRNGRAA"
BASE COUNT          623 a         1356 c         1300 g          664 t
ORIGIN      
        1 tgcgcggcga gcgtcacctg ggaggtgatc cgcgcgcgct gcgtcgcctc gctggcatag
       61 ccgggcgggc tcgcatagcg cgaatcgaca tggaggcagc ggccgtagaa atactcggcc
      121 ggctctttgt agagcttctc cgcctcggcg cggctctcgg cgacgccgac gaattggagg
      181 aagccggcgc ggtagggatt gcggtccttg ccgagccgct ccatctcgcg ccagaagccc
      241 atcatcgtct cgcgcccggc catgtatccg aaatacgaga ggtagcagta gacgtagtcc
      301 atctcggcgc accactgcca ggtctcgagc gagccgccgc ccgggatcca gatcggcgga
      361 tgcggcttct gcaacggctg cggccaaata ttgacatagc gcagctggtt gaagcggccg
      421 ttgaaggcga aggtctcggg ccgtgtccag gcctgaagca cgagatcgtg agcctcctgg
      481 tatttcgccc gcagcatgct cggattttgg ccataggcgt agcaggtatc catcggcgtg
      541 ccgaccggga agccggcgat gagccggccg ccggaaatgc aatcgagcat cgccatttcc
      601 tcggcgaccc gcgtcggcgg gttgtaaagc gcgagcgagt tgccgagcac gcagatcgcc
      661 gcatcgccgc cgcgccgcgc cagcatcgcg gcgatgagat tgggcgacgg catgagccca
      721 taggcgttgg cgtgatgctc gttgacgcag acggcgtcga atcccagggc ggcggcgtat
      781 tcgagctcgt cgatatactc attgtagagg cgatgggcat agacggggtc gaagagactc
      841 cggtcgagat caacccagac cgagttgtga cgctggcgga aatcctccgg cagccttgca
      901 tagggcatga ggtggaacca caggagcttc atggcgtcct tctcccgagc acggtcttgt
      961 gtccccggga tgttcccgcg aatcacattt gcgtgcaacc cgttgcaggc cgttgctcag
     1021 cgagggatgc tggtggcgag cgatgcgtag attggacgtc cacgcgccgc ccgcggcggc
     1081 gagcgcggat cggcgcaacc gatactgcgg agggtagaat gaagtccatg ctcgcggccc
     1141 tagcggccac tgtcgcggcg ttgaccacac cggtcgctgc ccaacaggcg cccccgccgc
     1201 ctccaggacc gccgcccgcg ccgccgcccg ccgcgtatcc gccggggccg ccgccggccg
     1261 cttatccgcc ggtgccgacg atcatgtggc tgcccggcgc ctatatccgc gccgacgccg
     1321 gctacgcctt caccttcgac acggacttcc gcgacgtgaa tttcgctcag accctggggg
     1381 acggcgtcag gatcaagggc gacagcggca actcgccctt ctacgaggcc ggattgggct
     1441 accgcttcac gcgctttttc cgcgccgacc tgaccgcgaa ctatcttccc tcgctcaaat
     1501 tcagcggcac ggacaatatc ggcctcggca ccgtcaacaa cgctcagatc cactccgaag
     1561 cggcctttgc caacgcctat ttcgatctcc cggcgatgct gacgatcttc gggcccttcg
     1621 tgcagcccta tttcgatgtc ggcgtcggcg cggcgcggga tcatgtcggc tccttctcct
     1681 cgaccttccc cggaatcgcc ggcaccatct cgccgcacac cacgacgcgc cccgccgtcg
     1741 ccttcggcct cggcacggcc atctcgctcg gccgcaactc ggtgctcgat ctcgcctatc
     1801 gcttcatgga tctgggcgag ctccggaccg gctctgccgt caccgccggc ggcgtcacga
     1861 ccgggatctc gccgatccat tcggagctct atgcgaacgt cgtgacgctg ggcatccgtt
     1921 tcgccttcta gaggcggcgg cccagatgcg gcggcggccg atgcgcgctt gaccttgagg
     1981 gcggccgcgt atcttaattc gtcggcagca cgacggagcc cgcatgcagt acgacctcaa
     2041 gatcgccggc ggcagcatca tcgacggcac cggcaaggcg cgctttgccg gcgatgtcgg
     2101 catcagcgcc ggcaaggtcg tggcgctggg ccaggcgccg ggggacgcgg tcgagacgat
     2161 cgatgcgacg ggccgcgtcg tcgcgccggg cttcgtcgac atccacaccc attacgatgc
     2221 gcagatcctc tgggaccaga ggctcaccat ctcgccctgg catggcgtaa cgaccgtggt
     2281 gatgggcaat tgcggcttcg gcatcgcgcc gacgcgacag gttgaccgcg accgcatcgt
     2341 ccgcacgctc gaaaaggtcg agggcatgag cgtcgcggca ctcgaagcgg ggctgggcgc
     2401 ggagtggggc ttcgcgacct ttccgcaata tctcgatgcc gtcgagcgca aaggcccggc
     2461 gatcaatgtc gcggcgctca tcggccacac cccggtgcgg ctcttcgcca tgggcgaggc
     2521 cgcgaccgag cgcgccgcga ccgcggagga agttgccgcg atgcgcggca tcgtgcgcga
     2581 cggcctcgac gccggcgcgc tcggcttcgc gacgtcgaaa gcgatcaccc atgtcggcta
     2641 tggcggcaag cccgtgccga gccgagccgc cgcattcagc gaggtcctgg cactcgcggg
     2701 cgcgctgcgc gaggccggca agggcgtgat ccaggcgacg atcggcccgg gcctgcagtt
     2761 cgacgagttc gcggcgctgg cgcgcgagag cggcgcccat gtgagctgga ccgcgctgct
     2821 cgcgggacgg ccgcgccagg gcgtcgacca ccgcgtgcaa ctcgcccgct cgctcgagct
     2881 ggcgcggcag aactacgccg tgttcccgca ggtgacgccg cggccgctga atttcgagct
     2941 tcagctcaag gagcccttcg tcttcgaaag catgtcgctg ttccgcccga tctcggccgc
     3001 cgatttcgcg ggcaagaagc ggctttatgc cgatgccgat ttccgcaagg cgttccgcga
     3061 caagatggac cgcgcgcccg gcgagttcgt gctgggattc cgcagcacga tgatctcgga
     3121 atacgcgccc gatcccagcc tcgaggagcg gcggctggtc gacgtcgccg cggagcgggg
     3181 cgtccatccc gtcgatctca tgctggatct cggcctcgcg accgacctcg acgcgcgctt
     3241 ccgcatggcc gtcgccaaca acaacgagga cgacgtcgcg gagctcctgc tcgacccgca
     3301 tatggtcatg gggttgagcg atgccggcgc gcatgcgagc cagctctgtg acgcctgcca
     3361 ggcgacctat ctcctggggc gctgggtgcg cgagaagcag gcgttgagcc tcgagcaggc
     3421 gatccgcatg ctgacctcgc gcccggccga agtcttcggc atcgccgatc gcggccgtct
     3481 cgccgcaggc tggccggccg atatcgtcgt cttcgatccc gccaccgtcg gcgccgggaa
     3541 gctgcgccgc gtctgggact tccccggcgg cgccaatcgc ctcgtgtccg acgccagcgg
     3601 catcgacgcg gtcatcgtca acggcacggt gctgcgccag aacggccgcg accgcgtcgc
     3661 gcccgacggg aaacttcccg gcaagctcct gcgcaacggc cgcgcggctt agaagaaaga
     3721 ttcaccactg aggcggtgag gcagtgagaa gaagattcgg cgcgcaaagc gcgtcaagtt
     3781 tcgttctctc ttctcactgc ctcaccgtct cggtggtgaa tcttcttctc tcttcttact
     3841 tcagttctcg cgttccggcc gaccgcggtg ccgcatcgcc tcccactctt cttcgctcaa
     3901 gcgctcgaga tgcgagaaag tgccggcgcc tttgagccag cgc
//
LOCUS       DASYNS010000374        17101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4307154, whole genome shotgun sequence.
ACCESSION   DASYNS010000374 DASYNS010000000
VERSION     DASYNS010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 17101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17101
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4307154"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VGP50_11635"
     CDS             complement(<1..936)
                     /locus_tag="VGP50_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028076.1"
                     /translation="MPRTAPRRLVTAAPLLEVGNLSVELATERGPLRAVRGVDLALAP
                     GRALGIVGESGSGKTMLARAILRLLPPAAKTTGRIALDGRDLYALSKKALRQLRWREL
                     AVVFQDPMTSLNPVLTIGSQIVEALTEHLGYSRARAFARAVELLTEVGIPEPERRARQ
                     YPHQMSGGMRQRVAIAIALSCEPKLLIADEPTTALDVTVQAQILDLLARERRMRRMAM
                     ILITHDLGVVAGRTDEVAVMYAGRILEHAPTRLLFKSMRVPYTAALLAALPKLDAPAH
                     TPLAAIAGMPPDPTRLPPGCSFNPRCPPSRERCRRD"
     gene            complement(950..2380)
                     /locus_tag="VGP50_11640"
     CDS             complement(950..2380)
                     /locus_tag="VGP50_11640"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8028077.1"
                     /translation="MTLGVEEDIAGFDPLTVGIYGTGALASAALLFDTLTRIDDEGRV
                     QPRLALSWSASSDFKTWTFKLRPGVKFQDGTPFNAQAVAFNYQRMMNPDNHCRCAFFL
                     VGIDHVEAADDLTVVYHLRVPADLPPLLSPSASVNVFHSPKAIQELGTDYNRHPVGTG
                     PFRLKSWQAGDRIVFERNGDYWDAGHPYLDQVVIRPLPDAGTRFASLLAGDVDIIWDD
                     ITDDVVRARTIATLRVNDYVGSGVAAIVLNTKTPALSDVKVRQALRYAVDMQALSDNF
                     DHGIRKPARDPYGQGSFVTCGDTGVLPYDPSKAEALLKEHGAPVRLSFMVTAEPRGMA
                     LGQIFQEFWSAVGVSVTVDPVDQTTFITKSFQHAFEIGGWRIVDAADPDPQLYADFHT
                     GSAVNTASYSNAEVDHLLDDARANGDREKRVADYCKIARILNQDVPWIWGLENRYFSI
                     AKATLMGVHKQYSDTVDVADAWWEKK"
     gene            2738..2989
                     /locus_tag="VGP50_11645"
     CDS             2738..2989
                     /locus_tag="VGP50_11645"
                     /inference="COORDINATES: protein motif:HMM:NF023267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HEV8028078.1"
                     /translation="MQLLKRALIVLAPVLALQACQGLSDQDRALLTAANQNAEQAKQM
                     AQQALDAARAAQASANQAASDAKAANEKADRIFQRSLRK"
     gene            complement(3075..4598)
                     /locus_tag="VGP50_11650"
     CDS             complement(3075..4598)
                     /locus_tag="VGP50_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8028079.1"
                     /translation="MDDQREIVSFLSAGASYGVPGAPVERIATHISILFLVGDRAYKL
                     KRAICFSYLDYSTLDRRERFARRELELNRRTAPDLYLGLRAITRRPDGTLAFDGNGTV
                     LDWVLEMRRFAQSDLLDRLAESRDLSPQLMRDLTDAIAAFHDGAEITGDHGGRAALDE
                     TVAQNNANLLRSSPPLDRGEIAALEAASRAALTAVGTLPDERRTQGKVRRCHGDLHLR
                     NVVMLEGRPMLFDCIEFNEDFSCIDVLYDLAFLLMDLTHRGLVDLANVVFNRYLDLTA
                     DIHGLPALPLFLSARAGVRAHVLGALNRQTPSAETLKEAESYLTLARALLRSHTPRLI
                     AVGGRSGTGKSTLSQALAGGFPPAPGARVIRSDVLRKRLFNAAPEATLPPHAYDMVTN
                     QRVYDGLRDHALAALAAGYTAIVDAAFLRAEERDRIAAVAAEAGVTFLGLWLDAPGDV
                     LAARVAARRRDASDADRAVLRRQLAMDLGPILWHPVDASGDAAGMLAAARDATGRAG"
     gene            4841..5083
                     /locus_tag="VGP50_11655"
     CDS             4841..5083
                     /locus_tag="VGP50_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028080.1"
                     /translation="MMTSDELARDLRDIDELITALSASDGDLADVDDLHWLLERRRSV
                     SELLAIRRAQRGKKIVSLAMWRCGCATEAEQMAGIA"
     gene            complement(5153..5521)
                     /locus_tag="VGP50_11660"
     CDS             complement(5153..5521)
                     /locus_tag="VGP50_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028081.1"
                     /translation="MAAKVFVVDDDDAVRASLRALLEAYGMEVEDYASTQDFLHDYRQ
                     RRMRGRCLVLDQHLPGATGLDFLASEGAELDLPVILVTGRGDLAIRNRARALGAIGYF
                     EKPLDTDLLITAIAGAAGGA"
     gene            5845..8154
                     /locus_tag="VGP50_11665"
     CDS             5845..8154
                     /locus_tag="VGP50_11665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028082.1"
                     /translation="MYRFGIRTLIIGLAFAGGLLGLLAGGFVLLEFRRSEERIIRELL
                     LETSQAISLAIDGELGRIQQELTVLSRFDPAEVARFSVASSSAANPRDGHGGWVTLLD
                     PAGRQIFNARQATPAASIEPAESELVRSVVATGRPAISNIFVSPLVSRSVAVVGVPVT
                     REGKTKYVLTMTVPTEHFAEVFERVGLPADWIAALVDSRRVTVARLPAPLDQGDRADA
                     SALADATQEGEAAGFEHVEISGVPVDGAVTRSKLSGWSVGIGVPTALIEAPFSRSLIE
                     FGAAACLLALAGLVATWRLGRRVAQSLSSLSRAADALGCREMPLKVAAGVREIEQTAA
                     ALRDAAELMKRQSVDQEKAMELLRRANEDLERKIRDRVGELAVADRQLSQEIAQRRDA
                     EETLAQQRKMEAIGQLTAGIAHDFNNLLTAVIGNLELLRPKLSDGRARRLARDAASAA
                     ERAALLTKQLLAFGRKQRLETRVIAINDLVAGAAPLIEQAAGPFVAVELALDEDAGAV
                     AVDPAQMELALLNLAINARDAMPGGGRLIVETRLLRVAGEHPDVAVGDWVLVSVADTG
                     VGMSAEVTARAFEPFFTTKRAGAGSGLGLSMVYGLVKQLHGEVALVSAPGQGTAVKIY
                     LPRALDEDVQQSGDDAEPKTPSSRARLLVVDDNPPVLSFMAGVLSEAGYDVVRAATGP
                     QALDLFGDGQSFAAVVLDYAMPAMNGVAAAQAMLARRADTPLLLVTGFVDTLTSREWP
                     SEDILRKPFGPESLRQRVDSLVERSRGSR"
     gene            complement(8177..8944)
                     /locus_tag="VGP50_11670"
     CDS             complement(8177..8944)
                     /locus_tag="VGP50_11670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028083.1"
                     /translation="MAATDADGESDAPLNALRRLEVLGELSDGLAHDFNNLLAIILAN
                     LERLMERSQDPELQHQVTTAHDAAKRGERLVRSLLSFAHRKPLGRNRLDLNGVIRDME
                     ALLVHCLGPQIRLVTLLDPAACPIDAEASRTEMAILNLAVNARDAMPKGGILRVETAN
                     RTLSGEHDGLRGAFVALTVADTGCGMSPEVLARAFEPRFTTKPAGTGLGLPMVRSLAK
                     RVCGTVTLESAAGKGTIVTVYLPRCQSPMDAARTARQ"
     gene            9793..10833
                     /locus_tag="VGP50_11675"
     CDS             9793..10833
                     /locus_tag="VGP50_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV8028084.1"
                     /translation="MTGRRAALSAKQQAGPKCYEVKTYGRFGSVIHPISAAPFRSANW
                     QFAWHEPARRVPWVAALTLEGDSRRHRREIAIRLLRTALICCTTGTSRPAPQPFMSLA
                     ACHEEDARRCVGWHRSHPTDDRARIPGPMLRILLADDHQMIREGLRQLLEAHVDWQVC
                     AEASTGREAVELALKLRPQIVVLDLAMPELNGLEATRQIKKAMPNTEVLIFTVHEAEE
                     LTRDVLAAGARAYLLKSDASRQIVAAIEALSEHKPYFTWKVSKTMLEAYLKHGQIDKD
                     KATGFSRLTAREREVVQLLAEGRSNKAVSALLGISVKTVETHRAAVMKKLGVTSIAEL
                     VRYAIRNSVIDA"
     gene            11239..13893
                     /locus_tag="VGP50_11680"
     CDS             11239..13893
                     /locus_tag="VGP50_11680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8028085.1"
                     /translation="MQAGAAWAVLLLVPIALLLLDRAATRRADIEQKAGTLARSSAKS
                     LDGEFAAAKAMLASLAASPALEEDAVARLYAQTVGVPTPSGSWIVLLGRDGTQRFSTL
                     YPYGASLPPPSDAIKRSLAKILEGRHPDSFDLSPAPPHDVGFGVPVLRHGEVALVLAL
                     MMASESLSRLFDEIPLPARWCGVIRDGADAVILRWPAGAGAGTEAGEARERETCGENT
                     VSFLAQSGATGWSAAILVPMSELEAPMRHALALTGGGGVLVIAVMGFAFFAGARVNRP
                     SRGGVTADEERFSMMAETVPSILYVTDAGGRCEYINQRFCEYTGMTPGAALGLGWVDA
                     VHPEEQRRVIENLMRPAPADDLRLTELRLRARDGTYRWFLGRSRPLRDADGSVVKWFG
                     SSTDIDGLKQSGTTLQRINAQLSAVLSGIDECYFTIDAECRITYINANAARFFGGTAD
                     ALLGRVLWEVAPELRDSEVEAEFTRALRERVPIQLERRSLIKPERWLAVNCYPWTEGL
                     SIFFRDITRRKRTEIALRRTQELLQRTMDALSAQIAILDEKGVIIAANSAWRRLSGHM
                     GCGVGRNYLAACATIVPDAGQAQKMTAALSAVLEAEQQEALVYYARPADTGSSWFQMR
                     ATRFDSAQGLRVVVAHEDITEIKQAETGLRDLTSRLLRVQDDERRRMAREVHDTTAQT
                     LTAAILKLDCLRNTVPALDQAPEEVWEELRALIDCALQETRTFSYLLHPPLLDELGLT
                     SALRWYVRGFEGRSRIAVSLTVQENLGRMPGTVESTLFRVVQEALTNIHRHSGSPTAQ
                     IRLTRSGEDVVLEIRDQGRGMARPDGADPALLGVGVSGMRARLHQLGGELAIRSTANG
                     TTVTAAVSLERLRSISAGDELASRAVSE"
     gene            14070..14798
                     /locus_tag="VGP50_11685"
     CDS             14070..14798
                     /locus_tag="VGP50_11685"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV8028086.1"
                     /translation="MPPDRRRTDDGFDNGILSALPRESAERLALNLETVDLSQGATIG
                     HADAPIDYIYFVHRGLVSLVKTLEDGQSIEVRTVGIEGMIGLPVLLSGEHAFTDYIVE
                     VPGFASRIVPRLLQDEVKRDRALAELLQQYAHAAFAQFVHMAACNGLHSVRERCCRWL
                     LVAHDNAGSDGFPLTHETLAALLGVQRASVSNVAHSLRQAGLIDYRHGSVTIENRSGL
                     EREACECHRILRAQFRHLASGAEH"
     gene            15061..15645
                     /locus_tag="VGP50_11690"
     CDS             15061..15645
                     /locus_tag="VGP50_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028087.1"
                     /translation="MEHQASLRGGRDRGPPVPRRRKSSRPLLAGKVMEAFSSVPATAM
                     VVDLDEQASDVVAGVLASLGCRVITTRDGESALATLKTEWPVDLLLTDVLLRGSIDGP
                     ELARLAIETNPSIEVIYSTSYSPMFLLDSEAPRDRLLLRKPWQSGKLKTILSCVLASQ
                     RSAARASADGACRPDMSRAVAAGEEWSLIADHGV"
     gene            complement(15666..16415)
                     /locus_tag="VGP50_11695"
     CDS             complement(15666..16415)
                     /locus_tag="VGP50_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8028088.1"
                     /translation="MIKLRNRVVLVTGAASGVGRKIVRNLLREGANVVIADLDPAAAE
                     AEAAAAELGGAENRVIGVAMDVTNEAQVDAGMQIAVDRFGRLDVLLATAGIRMIAPPD
                     RRVFGERERKLAIHFDGAFLPTRAALRQMCKQGSGGVIVHMGSTHSEDHESIAPANDV
                     AREEARPGIAAAAVCPAFMLMSTADGQIAEQAKGLGILERRAVDMIILKETVDGASTT
                     RDDAPEAVLVIAVLRSNAPTGQSRIVGRFFR"
BASE COUNT         2936 a         5621 c         5653 g         2891 t
ORIGIN      
        1 gtcgcggcga cagcgctcgc ggctcggcgg gcagcgcgga ttgaaggagc agccgggcgg
       61 cagccgcgtc ggatcgggcg gcatgccggc aatggccgcg agcggcgtat gcgccggcgc
      121 atcgagcttc ggcagcgccg ccaggagcgc cgccgtgtag ggcacgcgca tgctcttgaa
      181 gagcagccgt gtcggcgcat gctcgagaat gcgccccgca tacatcaccg cgacctcgtc
      241 ggtccggccg gcaacgacgc ccaggtcatg ggtgatgagg atcatcgcca tgcggcgcat
      301 gcgccgctcc cgcgccagaa gatcgaggat ctgcgcctgc acggtgacgt cgagcgccgt
      361 ggtcggctcg tcggcgatca gcagcttggg ctcgcaggag agcgcgatgg caatggcgac
      421 gcgctggcgc atgccgcccg acatctggtg aggatattgc cgcgcccgcc gctcgggctc
      481 gggaatgccg acctccgtca gcagctcgac ggcgcgggcg aatgcccgag cccgcgagta
      541 tccgagatgc tcggtgagcg cctcgacgat ctggctgccg atggtgagca ccggattgag
      601 cgaagtcatg ggatcctgga agaccaccgc gagctcgcgc cagcgcagtt ggcgtagcgc
      661 cttcttcgag agcgcataga gatcgcggcc gtcgagagcg atgcggcccg tcgtcttcgc
      721 cgcaggcggc agaaggcgca ggatggcgcg ggccagcatc gtcttgcccg agcccgattc
      781 gccgacgatg ccgagcgcgc gtcccggcgc cagcgccaga tcgacgccgc gcaccgcgcg
      841 caacggaccg cgctcggtcg caagctcgac gctgaggttg ccgacctcga gtaaaggcgc
      901 cgccgtcacg agacgccgcg gcgcggttcg aggcatcgcg cttttcgccc tatttctttt
      961 cccaccacgc gtccgccacg tccacggtat cgctgtattg cttgtgcacg cccatgagcg
     1021 tcgccttggc gatcgagaag tagcgattct ccagacccca gatccacggc acgtcctggt
     1081 tcaggatgcg cgcgatcttg cagtagtccg cgacgcgctt ctcccgatcg ccattggcgc
     1141 gcgcatcgtc cagaaggtgg tccacttcgg cattcgaata gctcgcggtg ttgacggcgc
     1201 taccggtgtg aaaatcggca tagagttgcg ggtcggggtc tgccgcgtcg acgatgcgcc
     1261 agccgccgat ctcgaaggcg tgctgaaacg acttcgtgat gaaggtcgtc tgatcgacgg
     1321 gatcgaccgt gacgcttaca cccaccgcgc tccagaattc ctggaagatc tggccgagcg
     1381 ccatgccgcg cggctccgcc gtcaccatga agctgagccg aacgggcgcg ccatgctcct
     1441 tgagaagtgc ttcggccttt gacggatcat aggggaggac gccggtgtcg ccgcaagtga
     1501 cgaaggagcc ctgcccatag ggatcccgcg ccggcttgcg aatgccgtga tcgaaattgt
     1561 ccgagagcgc ctgcatgtcg acggcatagc gcagcgcctg ccgcaccttg acgtcgctca
     1621 gcgccggcgt cttcgtgttg agcacgatcg ccgcgacgcc ggacccgaca tagtcgttga
     1681 cgcgcagcgt cgcgatggtg cgagcccgca cgacgtcgtc cgtgatgtcg tcccagatga
     1741 tgtcgacatc gcccgcgagc aggctcgcaa agcgcgtgcc ggcgtcgggc aagggccgga
     1801 tcaccacctg gtcgagatag ggatggcccg catcccaata atcgccgttg cgctcgaaca
     1861 cgatgcgatc gccggcctgc caggatttca gccggaaggg gcccgtaccc acgggatggc
     1921 ggttgtagtc cgtgcccagc tcctgaatcg ccttgggcga gtggaagacg ttgacggagg
     1981 cggacggcga cagcagcggc ggcaggtcgg cgggaacgcg cagatggtag acgaccgtca
     2041 ggtcgtcggc ggcttccaca tgatcgatgc cgacgaggaa gaacgcgcag cggcaatgat
     2101 tgtcgggatt catcatgcgc tgatagttga aggcgaccgc ctgagcgttg aaaggcgtgc
     2161 cgtcctggaa cttgacgccc ggtctgagct tgaaggtcca ggtcttgaag tcggacgacg
     2221 cgctccacga gagcgccagc cgcggctgca ccctgccctc gtcatcgatg cgcgtgagcg
     2281 tgtcgaagag caacgcggcc gaagcgagcg cgcccgtgcc gtagatgccg acggtgagcg
     2341 gatcgaagcc ggcgatgtcc tcctcgacgc cgagggtcac ctggccaccc cgagtttgcg
     2401 cttcggccac accgcaaagg aggagcacgg ccgcgccggc gaaaaggatc gccgcagaca
     2461 caccacgacc acgacgccgg tgcatcgcgc tcaccctccc ctcgcgcggc ccggttcgat
     2521 gtggccgttt cgccgcagga tacccgaata tgccggcgtt cggcaatcgg gtcgcggcgc
     2581 ttcatattgc ggcttgcggc cggccattac cgatcgcgac acgagagaag cgatgcccaa
     2641 tcaagcgatt ggcagctcgt cgcctgtcgc cttcttggcg attgacgcgg cgccgcacga
     2701 cggcaagatt ccgacgctca tctcaacgga gaacgatatg caactcctga aacgcgccct
     2761 gatcgtcctt gcgcccgtgc tcgcgcttca ggcctgtcag ggcttgagcg accaggaccg
     2821 ggcattgctc accgcggcga atcagaacgc cgagcaggcg aagcagatgg cgcagcaagc
     2881 gctcgacgcg gcccgcgcgg cgcaagcctc ggccaatcag gcggcgagcg acgccaaggc
     2941 cgcgaacgag aaggccgatc gcatcttcca gcgctcgctc agaaagtaag ccggacgggc
     3001 ggggccgccg cctcgccctt cgccggccgc tttgacggcc gtcaaagcgc ggcacccctg
     3061 accttcgaac cttgctagcc cgcgcgcccg gtcgcgtcgc gtgccgccgc gagcatgcca
     3121 gccgcgtcgc cgctcgcatc gacggggtgc cagagaatcg ggccgagatc catggcgagc
     3181 tggcgtcgca gcacggcgcg gtcggcatcc gacgcatcgc ggcggcgggc agcaacgcgc
     3241 gcggcgagga cgtcgccggg cgcgtcgagc cacaagccca ggaacgtcac cccggcttcc
     3301 gccgccacgg cggcgatccg gtcccgctct tccgcgcgca ggaaagcagc gtcgacgatc
     3361 gcggtataac cggcggcgag agccgccagc gcgtgatcgc gcaaaccgtc atagacgcgc
     3421 tggttcgtta ccatgtcata ggcgtgcggc ggaagcgtcg cctcgggcgc cgcgttgaac
     3481 aggcgcttgc gcagaacatc gctgcggatg acgcgggcac cgggcgccgg cgggaagccg
     3541 ccggccagcg cctgtgagag cgtggatttg ccggtgccgc tccggccgcc gacggcgatg
     3601 agccgcggcg tgtgcgagcg gaggagcgcg cgagcgagcg tcaaatagga ctccgcctct
     3661 ttcagcgtct ccgccgacgg ggtttgccgg ttgagcgcgc ccaggacatg ggcccgcacg
     3721 cccgcgcggg cggacaggaa cagcggcagc gccggcagcc catggatgtc cgcggtgaga
     3781 tcgaggtagc ggttgaagac gacattggcg agatcgacaa gaccacgatg cgtcagatcc
     3841 atcagcagaa aggccagatc atagagcacg tcgatgcagg agaagtcttc gttgaactcg
     3901 atgcagtcga agagcatcgg ccgtccctcg agcatgacga cgttgcgcaa atgaagatcg
     3961 ccatggcagc ggcgaacctt tccttgcgtg cgtcgctcgt cgggcagcgt tccgaccgcg
     4021 gtgagcgccg cccgcgacgc ggcctcgagc gccgcgatct cgccgcgatc gagcggcggc
     4081 gaggaccgga gcaggttggc attgttctga gcgacggttt cgtcgagcgc cgcccgtccg
     4141 ccatggtcgc cggtgatttc ggctccgtca tggaacgcgg cgatggcgtc ggtcagatcg
     4201 cgcatgagct gcggcgagag gtcgcgcgat tcggcaagcc ggtcgagcag atcactttgc
     4261 gcgaaacgcc gcatctccag cacccagtcg aggaccgtgc cattgccgtc gaaggccagc
     4321 gtgccgtccg ggcgccgggt gatcgcgcgg agcccgagat agagatcggg cgcggtccgc
     4381 cgattgagct cgagttccct tctggcgaag cgctcgcgcc ggtcgagcgt cgaatagtcg
     4441 agataggaga aacatatggc gcgcttcagc ttgtaggcgc gatcgccgac caggaagagg
     4501 atcgagatat gggtcgcgat ccgctcgacc ggggcgcccg gcacgccata gctcgcgccc
     4561 gcggagagaa aggatacgat ctcccgctgg tcatccatcg cgcgtctccc agcaagcgcc
     4621 tgaacagagg catgaaccaa tgtctattgt cgatcctttc taagggcgca ggtgctggca
     4681 tgcgcccgat cagcagagcc gagcatgatg cgaccggcca cgctttgatc tacctcaacg
     4741 gcgcagcaga tccgacgcgg gcggttgatc tggctcaagg cgcgccagcg gcgacgctca
     4801 ttgaatagga gccgggaaat acccgcaagg gagtgccgcg atgatgacgt ccgacgaact
     4861 ggcccgggat ctccgagaca tcgacgagct gattacggcg ctgagcgcgt ccgacggcga
     4921 cctcgccgat gtcgacgacc tgcattggct cttggagcga cggagatcgg tctcggagct
     4981 gctggccatc cggcgcgcgc agcgcggcaa gaagatcgtc agcctcgcca tgtggcgctg
     5041 tggttgcgcg accgaggccg aacaaatggc cgggatcgcc tgacgcatcg gcgtacccgt
     5101 acgctgcaga tcagggattc tacgtacccc ggaaggggga tggcgggcgc gctcaggcgc
     5161 cgccggccgc accggcaatg gccgtgatca gcagatcggt gtcgagcggc ttttcgaaat
     5221 agccgatggc gcccagagca cgggcgcggt tgcggatcgc gaggtcgcct cgtcccgtga
     5281 ccaggatcac gggaagatcg agctcggcgc cctccgaagc gaggaaatcg agccccgtcg
     5341 cgcccggcag gtgctggtcg agcaccaggc atcggccccg catccgccgc tgccgatagt
     5401 cgtgcaggaa atcctgcgtc gaggcatagt cctcgacttc catgccataa gcctcgagca
     5461 gcgccctgag ggaggcccgg acggcatcgt cgtcatcgac gacaaaaact ttggcggcca
     5521 ttgcctgcat cgcgacgcac ccgctttttc tcagggtttt actgtaggcc gccgcgcgac
     5581 gccgcgatat acggagtaat ccttacaggg ccgacgcgaa gagatgggcg tcgaaagaac
     5641 aatccgcgtg cccgctgtat cgcccgtcgc ccacggggat cgatgcacgg gcggcgcagg
     5701 ggaattccct gaaaagagtg gggagtcctg ctgatgttgc ttcgacccgc gctcgctgac
     5761 actggcatgt atcgcgggcg ccgcgaccgg gacgctgcgc cgtcggcaat gcccgccttc
     5821 ccgcgatccg gcaggaacaa ttggatgtat cgcttcggca tcagaacgct gatcattgga
     5881 ctcgcgttcg ccggtggcct cttgggattg ctggccggcg gcttcgttct cctcgagttc
     5941 cggcgcagcg aggagcgcat catccgagaa ttattgctcg agacgtccca ggcgatctcg
     6001 ctcgcgatcg acggcgagct cgggagaatc cagcaagagc tcaccgtcct ctctcgattc
     6061 gatcccgccg aggtcgcaag attttcggtc gcttcaagct ctgcggcaaa tccgcgcgac
     6121 ggccatggcg gttgggtcac cttgctcgac ccggccgggc ggcagatatt caacgcgcgg
     6181 caagcgacgc cggcggcttc gatcgagcct gccgaaagcg agctcgttcg cagcgtcgtc
     6241 gcgacgggcc ggcctgccat atcgaacatc ttcgtctcgc cactggtctc gcgctctgtc
     6301 gcggtcgtgg gcgtacccgt gacgcgcgag ggcaagacaa aatacgtgct gaccatgacc
     6361 gtgccgaccg agcattttgc cgaagtgttc gagcgcgtcg gtctgccggc ggactggatc
     6421 gcggccctcg tcgacagccg ccgcgtcacg gtggcacgcc tcccggcgcc gctggaccag
     6481 ggagaccgag cagacgcatc cgctctcgcg gacgcgacgc aggagggcga ggcggcgggc
     6541 ttcgagcatg tcgagatctc cggcgttccg gtcgacggcg ccgtcacgcg ctcgaagctt
     6601 tcgggctggt ctgtcgggat tggcgtcccc acggcgctga tcgaggcccc tttctcgcgc
     6661 tccctgatcg agttcggcgc cgccgcttgt ctcctcgcgc tcgccggcct ggtggcgacg
     6721 tggcgcctcg gccggcgcgt cgcgcaatcg ctgtcctcgc tgtcgcgtgc cgccgacgcg
     6781 cttggctgcc gcgagatgcc cctcaaggtc gccgccggcg ttcgcgagat cgagcagacc
     6841 gcagccgccc tgcgcgacgc ggcggaattg atgaaacggc aatccgtgga ccaggaaaaa
     6901 gccatggagc tgctgcgccg ggcgaacgaa gatctcgaac gcaagatcag ggaccgagtg
     6961 ggcgagctgg ccgtcgcgga tcgccaattg tcgcaggaga tcgcccagcg ccgcgacgcc
     7021 gaggaaacat tggcgcaaca gcgaaagatg gaagcgatcg gccagctcac cgccggcatc
     7081 gcccatgact tcaacaatct tctgacggcc gtcatcggca acctcgagct gctgcggccg
     7141 aagctctcgg acgggcgcgc gcgcaggctc gcccgggacg ccgcaagcgc agccgagcgc
     7201 gccgcgctgt tgaccaagca gctcctcgct ttcggccgca agcagcgtct cgagacacgc
     7261 gtcatcgcca tcaacgatct ggtcgccggc gcggcacccc tgatcgagca ggcggccggg
     7321 cctttcgttg ccgtcgagct cgccctcgat gaggatgccg gcgcggtcgc ggtcgatccg
     7381 gcccagatgg agcttgctct gctgaacctc gcgatcaacg cgcgcgacgc gatgcccgga
     7441 ggcggccggc tcatcgtcga gacacggctt cttcgcgtcg ctggcgagca tccggacgtc
     7501 gccgtcggag actgggtgct ggtctccgtc gcggacacgg gcgtcggcat gtcggccgag
     7561 gtcacggcgc gcgccttcga accgttcttc acgaccaagc gagccggcgc gggaagcggc
     7621 ctcgggctga gcatggtcta tggcctcgtg aagcaattgc acggcgaagt ggctctggtc
     7681 agcgcgcccg gccagggcac ggcggtgaag atctatctcc cgcgcgcgct cgacgaggac
     7741 gtgcagcaga gcggcgacga cgccgagcca aagacgccct catcccgggc gaggctgctc
     7801 gtcgtcgacg acaatccgcc ggtgctcagc ttcatggccg gggtcctgag cgaagcgggc
     7861 tacgacgtcg tccgcgccgc gaccggaccg caagcgctcg atctcttcgg cgatgggcag
     7921 agttttgccg ccgttgtcct cgactacgcc atgccggcga tgaacggcgt tgcggccgcg
     7981 caggccatgc tggcgcgccg cgccgatacg cccctcctcc tggtgacggg cttcgtcgat
     8041 acgttgacgt cccgcgagtg gccatcggag gacatcctgc gcaagccatt cgggccagag
     8101 agcctgcggc aacgcgtcga cagtctcgtc gagcggagcc gcgggagtcg atgacgaact
     8161 gtctggccgg atcgcgttac tgcctggccg tcctggcggc atccatcggg gattggcaac
     8221 gcggcaaata gaccgtcacg atcgtgccct tccccgcagc gctctcgagg gtcacggtgc
     8281 cgcagacccg cttggccagg ctgcgcacca tcggcaggcc gaggccggtg cctgcgggct
     8341 ttgtcgtgaa acgcggctcg aacgcgcgag cgagcacctc gggcgacatc ccacagcccg
     8401 tgtcggcgac ggtcaacgca acgaaggcgc cgcgaagccc gtcatgttcc ccggacaatg
     8461 tccgattcgc ggtctcgacg cgcaaaatgc cgcctttcgg catcgcgtcg cgcgcgttca
     8521 cggcaaggtt gaggatcgcc atctcggtcc ggctcgcctc ggcgtcgatc ggacaagcgg
     8581 cgggatcgag cagggtgacc agtcggattt gcggcccgag acaatgaacc agcagcgcct
     8641 ccatatcgcg gatgacgcca ttgagatcga gccggttgcg accgaggggc ttgcggtgcg
     8701 caaacgacag gagagacctg accagccttt cgccgcgttt ggcggcatca tgcgccgtcg
     8761 tgacctgatg ctgcagttcg ggatcctgcg agcgctccat gagccgctcg agattggcca
     8821 ggatgatggc gagcaggttg ttgaaatcat gcgccaggcc gtcgctcagc tcgcccagca
     8881 cctcgagacg gcgcaacgcg ttgagcggcg cgtcgctctc accgtcggcg tccgtcgctg
     8941 ccatcagctc ggatccaaga gccgacggcc gcgatctggc gtcgcgccct tcgggaaggt
     9001 cttcgcgacc aatccgtgcc aagttgtcca tccctgaggc aacgcaaacc ccgcccgatg
     9061 aaccgccaat accggagacc aggcgggcgc ccttggcaaa cattcatgat cgatgactcc
     9121 gcgatcctga agcgccttgc gcgaaagcag ggtcgcgcgc tgcggccggc ccgtgaatcc
     9181 ggtggaaccc tggcggggat gggaaaccgg ctgtacatcg attgcgacat tgcgagcagc
     9241 gtcgcgactg ccgatcgccg tcggcgcagc gcgaaacagg cgacgacgcc gccgctgttt
     9301 gccggcgtcg atctcccgtc gcggtcaccg cggcaacacc ggccgccgca gtcggcatac
     9361 gggcaatttc ttggccagcc cggggagtct cccgatttgc cgcgccaagc ttgtcggtta
     9421 gcgttgctgc gttgtcgtgc cacccctttc ccgcccaagg tggtacgtca acggtgctca
     9481 agaacgccgc gcggagccac aagctggctc cgcgcggatg ttcgcggtgt atcccacgga
     9541 aagtccggca cgccggcgga caaactgagg atttttccag cgccgaacac ccgattcacc
     9601 cgagagaccg gcgttccgga gcaccttatg ttcggcagcg tgcgattgtc atcacccccc
     9661 gccgatcgca cggccatctt ggtggtctgg tcgagagata gctgcgcagg ggtctcgagc
     9721 ccgagcccct gcgcagctgc tcccagtgaa acgcggaaaa agcattgcgc aggccaagcg
     9781 ccattcgcga cgatgacggg acggcgcgcc gcgctttctg ccaagcagca agccggaccg
     9841 aaatgctacg aggtcaaaac ctacggccgg tttggctcag tgattcaccc gatttcggcg
     9901 gcacccttcc gcagtgcaaa ctggcaattc gcctggcacg agcctgctcg gcgtgtaccc
     9961 tgggtcgcgg cgctgacgct ggagggtgat tcccggcggc acagacgcga aatcgccatt
    10021 cgcctgttgc gcaccgcgct catttgttgc acaaccggaa cgagtcgtcc ggcgccccag
    10081 ccgttcatgt cgttggcagc ctgtcatgaa gaagatgctc gacgttgcgt tggttggcat
    10141 cgctcgcatc caaccgacga ccgtgccagg atccccggac ccatgcttcg gatcctgtta
    10201 gccgacgatc atcaaatgat tcgcgagggg ttgcgccaac tcctggaggc gcacgtcgat
    10261 tggcaggtct gcgccgaggc atcgaccggg cgagaggcgg tggagctggc cctgaagctt
    10321 cgaccccaga tcgtggttct cgatctcgcc atgcccgagc tcaacggcct cgaggcaacg
    10381 cgtcagatca agaaagccat gccgaacacc gaggtcctga tcttcaccgt gcacgaggcg
    10441 gaagaactca cgcgggacgt gcttgcggcc ggcgcccgcg cctatctcct gaaatccgac
    10501 gcctcgcgac agatcgtcgc ggcgatcgaa gcgctgtcgg aacacaagcc ttacttcacc
    10561 tggaaagtgt cgaagacaat gctcgaggcc tatctgaagc acgggcagat cgacaaagac
    10621 aaggcgaccg gcttcagccg gctcaccgca cgagagcgcg aggtcgtcca gcttctcgcc
    10681 gagggccgca gcaacaaggc ggtctccgcc ttgctcggca tcagcgtcaa gaccgtcgag
    10741 acgcatcgcg ccgccgtcat gaagaagctc ggcgtaacct cgatcgccga gttggtgcgc
    10801 tatgccattc gcaacagcgt gatcgacgct tgagccgccg acgtcgtcgt ctttgcggtg
    10861 gcgaaactcg acgggcgcct cgtccgatgg cgtcgcgata ctcaccatta agaggggttc
    10921 ctcggcaaga gtatcaatgg tacactggat cggtgaattt ttctggggac ggctcggcag
    10981 gcgcatcgtg gatcgccagc acctgacggc cttggtcgct gtaagtcacc gtcgcgagat
    11041 tttgattctt tccggatgcg ggcgctccca tcgagagctg cctcggccat tgggtctggt
    11101 tgtttgcatt ttcgtattcg gggaaggcgg cggtcgctga tgactgtgga ctccgccctg
    11161 cgaagggatt cgagcggacg ctacgtgatc gttcgcaaag ccgctttgcg cgtgttcgat
    11221 ctgtcacgat cgcgatggat gcaggccggc gccgcctggg ccgttctctt gctggttccg
    11281 atcgctttgc tgcttcttga tcgggcggct acccggcgcg ccgatatcga gcagaaggcc
    11341 ggcacattgg cgcgtagctc cgcgaaatcc ctcgatggcg aatttgccgc ggccaaggcc
    11401 atgcttgcga gcctcgcggc atcgccagcg ctcgaagagg acgccgtcgc acggctctac
    11461 gcgcagacgg tcggcgtgcc aacgccatcc gggtcgtgga tcgtgctgct cggacgagac
    11521 ggcacgcaac gattcagcac gctctatccc tatggcgcat cgctgccgcc gccgagtgac
    11581 gcgatcaagc gatctcttgc caagattctg gaaggccgcc accccgacag cttcgatctt
    11641 tctcccgctc cgccgcacga cgtcggtttc ggcgtccccg tcctgcgcca cggcgaggtg
    11701 gccttggttc tcgccctgat gatggcgagc gagagcctct cgaggctctt cgacgagatt
    11761 cctcttccag cgcgatggtg cggcgtcatt cgcgacgggg ccgatgccgt gatcctgcgc
    11821 tggccggcgg gggcgggtgc cggcacggaa gcgggagagg cacgagagag ggaaacctgc
    11881 ggggagaaca cggtttcctt tctcgctcaa tccggcgcca ccggctggag cgccgctatc
    11941 ctggttccga tgtcggagct cgaagcgccg atgcgccatg cgctcgccct gacaggcggc
    12001 ggcggcgttc tggtgattgc ggtgatgggt ttcgcgttct ttgccggcgc gcgcgtcaac
    12061 cggccgtctc gcggcggcgt caccgccgac gaggagcgct tcagcatgat ggcggagacg
    12121 gtgcccagca tcctctatgt caccgacgcc ggcggcagat gcgagtacat aaaccaacgc
    12181 ttctgcgaat atacggggat gacgccagga gcggcgctgg gcctcggttg ggtcgacgcc
    12241 gtccatccgg aggagcagcg ccgggtcatc gagaatctga tgcgcccggc gccggccgac
    12301 gatctccgcc tcaccgagct gcgcctgcgc gcgcgcgacg gcacctatcg ctggtttctc
    12361 gggcggtctc gccccttgcg cgatgccgac ggctcggtcg tcaaatggtt cggctcgtcg
    12421 accgacatcg acggcctgaa gcaatcgggc acgacgctgc agcggatcaa cgcccagttg
    12481 agcgccgtgc tgtccggcat cgacgaatgc tacttcacga tcgatgcaga gtgccgcatc
    12541 acctacatca acgccaacgc cgcaagattc ttcggcggaa ccgccgatgc cttgcttggc
    12601 cgcgtcctgt gggaagtggc gccggagctc cgcgattccg aggtggaagc ggagttcacc
    12661 cgggcgctgc gcgagcgcgt gccgatccag ttggaacgcc ggtccttgat caagccggag
    12721 cgctggctgg ccgtgaactg ctatccctgg acagagggct tgagcatctt cttccgggac
    12781 atcacgcgac ggaaacgcac cgagatcgcg ctgaggcgca cacaggagct cctgcagcgg
    12841 accatggacg cgctctcggc gcagatcgcc atcctggacg agaaaggggt catcatcgcc
    12901 gccaactcag cctggcggcg cctgagcggg cacatgggct gcggcgtcgg ccgcaactac
    12961 ctcgcagctt gcgcgacgat cgttcccgac gccgggcaag cacagaaaat gaccgctgcg
    13021 ctcagcgccg tgctggaggc ggagcagcag gaggcgctcg tctattatgc gcgccctgcc
    13081 gacacgggct cgagctggtt ccagatgcgg gcgacccggt tcgacagcgc gcaagggctg
    13141 cgcgtcgtcg tcgcgcatga ggatatcacc gagatcaagc aggcggagac cggactgcgc
    13201 gacctgacca gccgcctcct tcgggtccag gacgatgagc gccggcgaat ggcgcgcgaa
    13261 gttcacgata cgaccgcgca aaccctgacc gccgccattc tcaagctcga ttgcttgcgc
    13321 aacaccgtcc cggctctcga tcaagccccg gaagaggtct gggaagaatt gcgcgcgctc
    13381 atcgactgcg ctctccagga gacccgcacc ttttcctatc tccttcatcc gcctcttctc
    13441 gatgagctcg gcctgacatc ggcgcttcgc tggtatgtgc gcggcttcga aggccgcagc
    13501 cgcatcgcgg tgtctttgac cgtccaggag aatctcgggc gaatgccggg gaccgtggaa
    13561 agcacgctct tccgcgtggt tcaggaagct ttgaccaaca tccatcggca ttccgggagt
    13621 ccgaccgcac agattcgctt gacccggtcc ggtgaggacg tggtgctcga gatcagagat
    13681 cagggtcgcg gaatggcacg gccggacggc gccgatcccg cgcttttggg cgtgggcgtc
    13741 tcgggcatgc gcgcgcggct gcatcagctc ggcggcgagc tggcgatccg ctcgaccgcc
    13801 aacggcacga cggtgacggc cgcggtgtcg ctcgagcgcc tgcgatcgat ttcggccggc
    13861 gacgaactcg cctcccgcgc tgtgtccgaa taaattgcct tccagcgtct tcctgcggca
    13921 accgcaggta agcgctgcgg caccgtcgtg ccttctgcag cgatcgacgt cctctcgtct
    13981 ggctcgattt cttttgcggt cacgtaaaaa ttaacattta aatgttgatc agcgatcgat
    14041 ttgcgtggca ctgaattggt gaggaaatcg tgccaccgga tcgaagaagg accgacgacg
    14101 gctttgacaa tggcatcctg tcggccctgc cgcgggagag tgccgagcgg cttgccctca
    14161 atctggaaac cgtcgatctg tcgcagggag cgacgatcgg ccatgcagat gcgccgatcg
    14221 actatatcta tttcgtgcac cgcggtcttg tttcgctcgt caagaccctc gaggacgggc
    14281 aaagcatcga ggtgcgcacg gtcggcatcg agggcatgat cgggttgccg gtgcttctga
    14341 gcggcgagca tgccttcacc gactatatcg tcgaggtgcc gggattcgct tcacgcatcg
    14401 tgccgcgcct gctccaggac gaggtgaagc gagatcgcgc gttggcggag cttttgcagc
    14461 aatacgcgca cgcggcgttc gctcaattcg tccatatggc cgcttgcaac gggctccatt
    14521 cggttcgaga gcgctgctgt cgttggttgt tggtcgctca tgacaatgcc ggatcggacg
    14581 gctttcccct cacccatgag acgctcgccg cgctgctcgg cgtccagcgc gccagcgtct
    14641 cgaacgtcgc tcactctttg cggcaggctg gcctgatcga ctatcgccat ggcagcgtga
    14701 cgatcgagaa tcggagcggc ctcgagcgcg aagcctgcga atgccaccgc atcttacgcg
    14761 cacaattccg tcatcttgcg tccggcgcgg agcactgata ctcgttttgc ggaagaaagt
    14821 ttgcatccga cgcactcgtc gggagaaacg gcacgtgctc gcgcgagaaa agagaaccgt
    14881 caaggagcta tttaatggca ctctgtacgg ttgcgtacag atcgtcgagc catctgcgtt
    14941 ctagagtgac ggcgggtcgt tgcaaggcga cccatcgata tctgcgcggg ccaggatcat
    15001 gccgccagcc tgtttcgcgc accgtgaacg ccggtatccg ccgcccgctc gacggatgcc
    15061 ttggagcatc aagccagttt gcggggtggc cgcgaccgag ggccgcccgt ccctcgccgg
    15121 aggaagagca gtcgaccgct gctcgctggc aaagtcatgg aagcattctc gagcgtcccc
    15181 gcgacagcca tggtggtgga tctcgacgag caggcatcgg acgtcgtcgc cggcgttctc
    15241 gcttcgctgg gctgccgggt aatcacgacg cgcgacggcg agagcgcgct cgcgacactc
    15301 aagaccgaat ggccggtcga tctgctcctg accgacgtcc tgctgcgcgg aagtatcgat
    15361 ggcccggagt tggcccgcct ggcgatcgaa acgaacccga gcatcgaggt catctacagc
    15421 accagctatt cgccgatgtt cctcctcgac agcgaggccc cgcgcgaccg gctgctgctt
    15481 cgaaagccct ggcagagcgg caaattgaaa acgatattgt cgtgcgtctt ggcgtctcag
    15541 cggtctgccg ctcgcgcatc agcggatggc gcttgccgtc ccgatatgtc gcgcgccgtg
    15601 gcggccgggg aagaatggag cctcatcgcc gatcacggcg tatagccccg acgcagcaga
    15661 tccggtcatc tgaagaagcg cccgacgatc cgcgattgcc cggtcggcgc atttgaccgg
    15721 aggaccgcta tgacgagcac cgcctccgga gcgtcgtccc gtgtcgtcga ggcgccgtcc
    15781 acggtctcct tgagtatgat catgtcgacg gcccgccgct ccaagatgcc gagccctttc
    15841 gcctgttccg cgatctgacc atcggcggtc gacatgagca taaaggccgg acagacggcg
    15901 gcggcggcaa tgccgggcct cgcttcttcc cgcgcgacat cattggccgg cgcgatagat
    15961 tcgtggtcct ccgaatgcgt cgagcccata tggacgatga cgcccccgct cccctgcttg
    16021 cacatttggc gcagcgcggc gcgggtcggc agaaatgcgc cgtcgaaatg gatggcgagc
    16081 ttcctctccc gctcgccgaa cacccgtcgg tccggcggcg cgatcatccg gatgccggcc
    16141 gtggcgagaa gcacgtcaag ccggccgaac ctgtctacgg cgatctgcat cccggcatcg
    16201 acttgtgctt cgttcgtaac atccatggcg acgccgatca cgcggttctc cgcgccgccg
    16261 agctccgccg ctgcggcctc ggcctcggcc gcggccgggt cgagatccgc gatcacgaca
    16321 ttggcgccct cgcgcagcag gttccgaacg atctttctcc cgacaccgct cgccgcaccc
    16381 gtcacgagca cgacgcggtt cctcagcttg atcatcgccc cctcttatgc cgcgaccgac
    16441 gtttcggaca cgataagcag caccttccca gcgatcagac attcccgagt gacgggccgc
    16501 ggatggttca ctgcaatttt ctttgtcccg aagcgcgccc gcgcgacatc gcaccaccgc
    16561 cgatatccgc acgaccgcaa caggcttaag ccttcgacga ttcagcggag cggcgaaacg
    16621 gtgtgccggg ggcggaccgt aacccgccgc tgatcgcgtc ggacatcggt gaaatcctga
    16681 tatcgcatgg aagttcacgg tagcccggca cggctattcc catgcgggat ctcggctttt
    16741 gatgcggtcc cgccggcggc ttggggcgcc gaagcgggtt tccgcgcgca cctcccgcat
    16801 ccggaatggc gtcgagcggc cccctttccc cggtcgaacc gccgggaacc cgggccccct
    16861 cttcatgcgc gcatacactc acgcgagcga tttattttcc tctgcccgat gccgcccctg
    16921 cgcggtggtg ctatatagtg tgatgggacg aatgccgatg aagacggacg gtttggtcgg
    16981 cacggcgaca atcgagcggc cttccagctg accagggtcg cgggcgagcg ccgggaagcc
    17041 agtttcgctg aataacagcg ccggtcgggg tcgtcattgc tgcagcggcg atcctcgggc
    17101 t
//
LOCUS       DASYNS010000375         4948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2747086, whole genome shotgun sequence.
ACCESSION   DASYNS010000375 DASYNS010000000
VERSION     DASYNS010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2747086"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="VGP50_11700"
     CDS             <1..1648
                     /locus_tag="VGP50_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV8028089.1"
                     /translation="VVAPDVGGGFGSKIYHYAEEAIVTWAAPKVGKPIKWTADRSESF
                     TSDAQGRDHHTKAELALDKDGKFLAMRVATIANLGAYLSTFGSAVPTYLYGTLLAGTY
                     TTPAVYVEVKSVFTNTVPVDAYRGAGRPEACYVVERLVDAAAREMKIDPVELRRRNFI
                     PKDAYPYQTPVALTYDSGDYFTTLDMALKASDYAGFAKRRDEAAKRGKLRGIGIAAYI
                     EACGIAPSAVAGALGARAGLYEMGEIRVHPTGSVTVFTGTHSHGQGHETTFAQLVADR
                     LGLDIAMVDVVHGDTAKIPFGMGTYGSRSLPVGGSALVKALDKVVNKGKKIAAHLLEA
                     AEADIEFKDGKFAVAGTDKSVPFAQIAFTAYVPHNYPITELEPGLDETAFYDPLNFTY
                     PAGAHICEVEIDRDTGVVRVVNFNAVDDFGNIVNPMIVEGQVHGGLAQGIGQALYENT
                     LYDEESGQLITGSFMDYCMPRADLLPSFTVATHGTPCTHNPLGVKGCGEAGAIGAPAA
                     VMNAVVNALAPLGISDLEMPATSERVWRAIAAHSMPKAAE"
     gene            1663..2460
                     /locus_tag="VGP50_11705"
     CDS             1663..2460
                     /locus_tag="VGP50_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEV8028090.1"
                     /translation="MHDFQYHKPQSVADAAKMIGANDEAKLLAGGMTLIPTLKQRLAR
                     PSDLVDLAKIPELKGIKRDGNAIVIGAMTRHVDVATSDVVKGAIPGLAHLAEGIGDPQ
                     VRNRGTIGGSIANNDPAADYPSAVLALGATVITNKRKIGADDFFKGMFETALAPDEIV
                     TAVSFPIPEKAGYSKFPNPASRYAIVGVFVAKTGNTVRVAVTGAAPCVFRQKDMEAAL
                     SKSFTSDAVAAIKQNQSGLNTDIHASAEYRAHLVTVMAKRAVAAALA"
     gene            2548..2943
                     /locus_tag="VGP50_11710"
     CDS             2548..2943
                     /locus_tag="VGP50_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEV8028091.1"
                     /translation="MDTGEIARRLRRDMTEAEKVMWRILRARQLAGYKFRRQEPIGRY
                     VVDFVCFSPRIVIEIDGGQHNEVTSYEERRSRFLEREGFMVLRFWNNEVLENREGVCE
                     RIVEVLSRITPHPPVASRRAPPSPTRGEG"
     gene            2989..3900
                     /locus_tag="VGP50_11715"
     CDS             2989..3900
                     /locus_tag="VGP50_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV8028092.1"
                     /translation="MSLPSSVSETLALLQRGNYVGDRSLATAVYLALALKRPLFLEGE
                     AGVGKTEIAKVLAATLGRRLIRLQCYEGLDVASAVYEWNYARQMIEIRLAEAESDSRA
                     TVEEEIFSDRFLIKRPLLQALEPDLAGPPVLLIDELDRTDEPFEAYLLEVLSDFQITI
                     PEIGTIKAKEPPVVVITSNRTREIHDALKRRCYYHWIDYPNASRELEILRVKAPGAAE
                     GLSREVVAFVQKLRAANDLFKLPGVAETIDWAHALSQLDVLALTPEAINDTLGVLLKY
                     QDDIVKIRGSEAQRLLTQVKQELAAAQ"
     gene            3897..>4948
                     /locus_tag="VGP50_11720"
     CDS             3897..>4948
                     /locus_tag="VGP50_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV8028093.1"
                     /translation="MSGRLVENVMHFARALRAAGLPIGPGKVLAAIEALGAVGIADRQ
                     DFYWTLHAVFVNRRDQRELFDQAFHIFWRNPQLLEKMLGIMLPTIRSDEPMEEMSRRL
                     AEALHAGDGKTREEPEPEKIELDAVMTFSDREKLQRMDFEKMSGEELRQARAALRRLT
                     LPLAEAPIRRFTLDPRGERADMRASLRAQLRSTGVIALKRKRRQKRPPPLVVLCDISG
                     SMSRYSRLFIQFMHAVTNDRDRVSTFLFGTRLTSITRYLRQKDVDVALDRVAAAVEDW
                     SGGTRIGQSLAEFNRRWSRRVLGQGAVVLLITDGLDREGGQGIAQEMDRLHRSCRRLI
                     WLNPLLRYEGFAPKSQG"
BASE COUNT          875 a         1706 c         1571 g          796 t
ORIGIN      
        1 cgtcgtcgcc cccgatgtcg gcggcggctt cggctcgaag atctaccatt atgccgagga
       61 ggcgatcgtc acctgggccg cccccaaggt cggcaagccg atcaaatgga cggccgaccg
      121 ctccgagagc ttcacctcgg atgcccaggg gcgcgatcat cataccaagg ccgagctcgc
      181 gctcgacaag gacggcaagt tcctggcgat gcgcgtcgcg acgatcgcca atctcggcgc
      241 ctatctctcg accttcggga gcgcggtgcc gacctacctc tacggtacgc tcttggccgg
      301 cacctacacg acgccggcgg tttatgtcga ggtcaagtcg gtcttcacca acacggtgcc
      361 ggtcgacgcc tatcgcggcg ccgggcggcc cgaggcctgc tatgtggtgg agcgcctcgt
      421 cgatgctgcg gcgcgcgaga tgaagatcga tcccgtcgag ttgcgccggc gcaacttcat
      481 cccgaaggac gcctatccct atcagacgcc ggtggcgctc acctatgaca gcggcgatta
      541 cttcacgacg ctcgacatgg cgttgaaggc ctcggattac gccggcttcg ccaagcgccg
      601 cgacgaggcg gccaagcgcg gcaagctgcg cggcatcggc atcgccgcct atatcgaggc
      661 gtgcggcatc gcgccttcgg cggtggcggg cgcgctcggc gcgcgcgccg ggctctacga
      721 gatgggagag atccgcgtcc atccgacggg gtcggtgacg gtgttcaccg gcacccacag
      781 ccacggccag ggtcacgaga ccaccttcgc ccagttggtc gcggaccgcc tcggcctcga
      841 catcgccatg gtcgacgtgg tccatggcga caccgcgaag atccccttcg gcatgggcac
      901 ctatggctcg cgctcgctgc ccgtcggcgg ctcagcgctg gtgaaggcgc tcgacaaggt
      961 cgtgaataag ggcaagaaga tcgccgcgca tctgctcgag gccgccgagg cggatatcga
     1021 gttcaaggac ggcaaattcg cggtcgccgg caccgacaaa agcgtgccct tcgctcagat
     1081 cgccttcacc gcctatgtgc cgcacaacta cccgatcacc gagctcgagc ccggcctcga
     1141 cgagacggcg ttctacgatc cgctgaattt cacctatccg gcgggcgcgc atatctgcga
     1201 ggtcgagatc gaccgcgaca ccggcgtcgt ccgtgtcgtg aacttcaacg ccgtcgacga
     1261 tttcggcaac atcgtcaatc ccatgatcgt cgagggccag gtccatggcg ggctcgccca
     1321 gggcatcggc caggcgctct acgagaacac gctctacgac gaagagagcg ggcagctcat
     1381 caccggctcc ttcatggatt actgcatgcc gcgtgccgat ctgttgccgt ccttcacggt
     1441 cgcgacccat gggacgccgt gcacgcacaa tccgctgggc gtcaaaggct gcggcgaggc
     1501 cggcgccatc ggcgcgccgg cggcggtgat gaacgcggtg gtgaacgcgc tggcgccgct
     1561 cggcatcagc gatctcgaga tgccggcgac gtccgagcgc gtgtggcgcg cgatcgccgc
     1621 gcacagcatg cccaaagcgg cggaataagg gagcgagcag ccatgcatga tttccaatac
     1681 cacaagccgc aaagcgtcgc cgacgccgcc aagatgatcg gcgccaacga cgaagcgaaa
     1741 ctgctggccg gcggcatgac gttgattccg accttgaagc agcgcctggc gcggccttcc
     1801 gatctcgtcg acctcgccaa gatccccgag ctcaagggca tcaagcgtga cggcaacgcc
     1861 atcgtcatcg gcgccatgac gcgccatgtg gatgtcgcga cctccgacgt cgtcaagggc
     1921 gcgatccccg ggctcgcgca tctcgccgag ggcatcggcg atccgcaagt gcgcaaccgc
     1981 ggcaccatcg gcggctcgat cgccaacaac gacccggcag cggactatcc atccgcggtt
     2041 ctggcgctgg gcgcgacggt gatcaccaac aagcgcaaga tcggcgccga cgatttcttt
     2101 aagggcatgt tcgagacggc gctggcgcct gacgagatcg tcacggccgt gagcttcccg
     2161 atcccggaga aggccggcta cagcaagttc ccgaaccccg cgtcgcgcta cgccatcgtc
     2221 ggcgtcttcg tcgccaagac cggcaacacc gtgcgcgtcg ccgtgaccgg cgccgcccct
     2281 tgcgtcttcc gccagaagga tatggaggcg gcactctcga aatccttcac ctccgacgcc
     2341 gtcgccgcca tcaagcaaaa ccagagcggc ctcaataccg acatccacgc cagcgccgaa
     2401 taccgcgccc acctggtaac ggtaatggcc aaacgcgccg tggctgcggc cctcgcctga
     2461 cggtgaagcg cagttttctc cctctcccct ctgtgggaga gggaggggcc catcgcgaag
     2521 cgatgggagg gtgaggggcg acggcgcatg gacaccggag aaatcgcacg ccgcctccgc
     2581 cgcgacatga cggaggccga gaaggtgatg tggcgcatcc tccgcgcccg ccagctcgcc
     2641 ggatacaagt tccgtcgtca ggaaccgatc ggccgctacg tcgtggactt cgtctgcttc
     2701 tcgccgcgga tcgtgatcga gatcgatggt ggccagcaca acgaggtcac ttcgtacgaa
     2761 gagcgccgct cacgcttcct cgaacgcgag ggattcatgg tccttcgttt ttggaacaac
     2821 gaagtgctgg aaaatcgcga aggcgtttgt gagcgcatcg tcgaggtgct ttcgcgcatc
     2881 acccctcacc ctcccgtcgc ttcgcgacgg gcccctccct ctcccacaag gggagagggg
     2941 taagtccgtt tccttgccag agatacggcg atcttctaag aaacgcgcat gtccctcccc
     3001 tcttccgtaa gcgaaacctt ggcgctgttg cagcgcggga attatgtcgg ggatcgcagc
     3061 cttgcgacgg cggtctatct tgcgcttgcg ctgaagcggc cgctgttcct cgagggtgag
     3121 gccggggtcg ggaagaccga gatcgccaag gtgctggcgg cgacactcgg acggcgattg
     3181 atccgtctcc aatgctatga aggcctcgac gtcgcctccg cggtttatga atggaactac
     3241 gcgcggcaga tgatcgagat ccgcctggcc gaggccgaga gcgacagccg cgctaccgtc
     3301 gaggaagaga tcttctccga ccggtttctc atcaagcggc cgctgctcca ggcgctcgag
     3361 cccgatctcg cgggaccgcc cgtgctgctc atcgacgaac tcgaccgcac cgacgagccc
     3421 ttcgaggcct atctcctcga agtcctctcg gacttccaga tcaccattcc cgagatcggc
     3481 acgatcaagg cgaaagagcc acccgtcgtc gtcatcacgt cgaaccgcac ccgcgagatt
     3541 cacgacgcgt tgaaacgccg ctgctactac cactggatcg actatccgaa tgcgtcgcgc
     3601 gagctcgaga tcctgcgcgt caaggcgccg ggcgcggccg agggcttgag ccgcgaggtc
     3661 gtcgccttcg tgcagaagct gcgcgccgcc aacgacctct tcaagctgcc gggtgtcgcc
     3721 gagacgatcg actgggcgca tgcgctctcg cagctcgacg tgctggcgct gacgcccgag
     3781 gccatcaacg acacgctggg cgtactgctc aaataccagg acgacatcgt caagatccgg
     3841 ggctcggaag cgcagcgcct gctcacccag gtgaagcagg agctggccgc ggcgcagtga
     3901 gcggccggct cgtcgagaac gtcatgcatt ttgcgcgggc actgcgcgcc gccggcctgc
     3961 cgatcgggcc gggcaaggtg ctcgccgcga tcgaagctct gggcgccgtc ggcatcgccg
     4021 accgccagga tttctactgg acgctccacg ccgtcttcgt caaccgccgc gaccagcgcg
     4081 agcttttcga ccaggctttc cacatcttct ggcgcaatcc ccagctcctc gagaagatgc
     4141 tgggcattat gctgccgacg atccgcagcg acgagcccat ggaagagatg agccgccgcc
     4201 tcgccgaggc gctgcatgcg ggcgacggca aaacccggga ggagccggag ccggagaaga
     4261 tcgagctcga cgcggtcatg accttctccg atcgcgagaa gctgcagcgc atggatttcg
     4321 agaagatgtc cggcgaggag ctgcgccagg cgcgcgccgc gctccgccgc ctgacgctgc
     4381 cgctggcgga agcgccgatc cgccgcttca cgctcgaccc gcgcggcgag cgcgccgaca
     4441 tgcgcgcgag cctgcgcgcc cagctgcgct cgaccggcgt catcgccttg aagcgcaagc
     4501 gccggcaaaa gcgcccgccg ccgctggtcg tgctctgcga tatctccgga tccatgagcc
     4561 gctacagccg gctcttcatc caattcatgc acgccgtcac caacgaccgc gaccgcgtct
     4621 cgaccttcct gttcggcacg cggctcacca gcatcacgcg ctacctgcgc cagaaggacg
     4681 tcgacgtggc gctcgaccgc gtcgccgccg cggtcgagga ctggtcgggc ggcacgcgca
     4741 tcggccagtc ccttgcggaa ttcaatcggc gctggtcgcg ccgcgtcctg ggccagggcg
     4801 cggtggtgct gctcatcacc gacggcctcg accgcgaggg tggccaaggc atcgcccagg
     4861 agatggaccg cctgcaccgc tcctgccgcc gcctgatctg gctgaacccg ctgttgcgtt
     4921 acgagggttt cgcgccaaaa tcacaggg
//
LOCUS       DASYNS010000376         4498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4932784, whole genome shotgun sequence.
ACCESSION   DASYNS010000376 DASYNS010000000
VERSION     DASYNS010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4932784"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2163
                     /gene="carB"
                     /locus_tag="VGP50_11725"
     CDS             <1..2163
                     /gene="carB"
                     /locus_tag="VGP50_11725"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV8028094.1"
                     /translation="FEKFPGATPLLTTSMKSVGEAMAIGRSFAESVQKALRSLETGLT
                     GFNEVEIPGAAESDRNAVRAALAMPTPDRLLVIAQAYRHGLTTAEIHAACKYDPWFLE
                     EVRRIVLAEAEIRAKGLPENASGLLRLKQQGFADARLAELAGCEEAAVAARRAKLGIH
                     PVYKRIDTCAAEFPSLTPYMYSTYEGDGASPPECEAEPSERRKVVILGGGPNRIGQGI
                     EFDYCCVHAAYSLKEAGFETIMVNCNPETVSTDYDTSDRLYFEPLTAEDVIELVRVEE
                     RRGTLLGVVVQLGGQTPLKLAAALEAARIPILGTSPDAIDLAEDRRRFQELLHRLDLK
                     QPENGIASSVEEAEKVASRIGYPLVIRPSYVLGGRAMEIVHGPAGLTRYMTNAVKVSG
                     KNPVLIDRYLQDAIEVDVDALADGSTVAIAGIMEHIEEAGIHSGDSACSLPPYSLPAP
                     VIADIERQTAALAKALGVVGLMNVQYAVKEQTVYVLEVNPRASRTVPFVAKATGVPIA
                     KIAARVMAGERLDAFKLDRSRRRHVAVKEAVFPFARFPGVDLILGPEMKSTGEVMGLD
                     TDFGRAFAKSQLGSGVDLPLQGTVFVSVRDRDKPAMVQPCRRLVEMGFTLLATAGTGE
                     RLREAGLPVEAINKVLEGRPHIVDRMLSGGVQLVFNTTEGAQAIADSFSLRRTALTNA
                     IPYYTTVAGARAAVEAIAALRAGSLEVASLQSYFVGSF"
     gene            2248..2721
                     /gene="greA"
                     /locus_tag="VGP50_11730"
     CDS             2248..2721
                     /gene="greA"
                     /locus_tag="VGP50_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV8028095.1"
                     /translation="MNKIPMTETGYNRLQEELKRLKSVDRPAVIRAIAEARENGDLSE
                     NAEYHAARERQSFIEGRVLELEDKIARAEVIDVSKLSGKLVKFGATVTLADEETDEKV
                     SYQIVGEDEADISQRRLSVTSPLARALIGKAIGDSVEVSTPRGARSYEIVKVGFR"
     gene            complement(2750..3115)
                     /locus_tag="VGP50_11735"
     CDS             complement(2750..3115)
                     /locus_tag="VGP50_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028096.1"
                     /translation="MEISRRVRDSDDDAFDHAAHEFLEVFYGQPEEREAALADRPEPL
                     DPVRDAYLAAVAEHLARSFHLPTPEWSETHGNNLIRPIFAGDESMIATLTVHSPTAFR
                     RRLLFVSKDTLAPPRRTQD"
     gene            complement(3145..3828)
                     /locus_tag="VGP50_11740"
     CDS             complement(3145..3828)
                     /locus_tag="VGP50_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEV8028097.1"
                     /translation="MSMPKTSGSPFDLLIFDCDGVLIDSETIVCRVEVQALAEIGYDL
                     ELERFMERFVGKAARDGRALIEAELGRKLPAGFEAETARRVAEAFARELKAVAGVDDA
                     LAAIPGRRCVASSSLPERLAYTLGLTGLAKWFGDAVFSVSMVARGKPAPDLFLHAARA
                     MAADPARCLVIEDSAPGITAAKAAGMMALGFMGASHCRPGHDARLAAAGADLVFSDMR
                     ELPRLVATT"
     gene            complement(3809..4294)
                     /locus_tag="VGP50_11745"
     CDS             complement(3809..4294)
                     /locus_tag="VGP50_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEV8028098.1"
                     /translation="MRRIKLDKIDRRILRDLQATGRMTNVDLAKRAGISAPPCLRRVR
                     ALEEAGFIRGYHADVNPEALGFGVTVFAQVGLSSQAEPDLKAFEVLALTWPEVREVHM
                     LAGETDFLLKIVATDWDSYQRFLTTKLTAAPNVAHVRSSLAIRASKYEPGVPIDVDAK
                     D"
BASE COUNT          750 a         1551 c         1486 g          711 t
ORIGIN      
        1 ttcgagaaat tccccggcgc gacgccgctt cttacgacgt cgatgaaatc ggtcggcgag
       61 gccatggcca tcggccgctc cttcgccgag tccgtgcaga aggcgttgcg ctcgctcgag
      121 accgggctca ccggcttcaa cgaggtcgag atcccgggcg ccgccgagag cgaccggaac
      181 gccgtccgcg ccgcgctggc aatgccgacg cccgaccggc tgctggtgat cgcccaggcc
      241 tatcgccacg gcctcaccac cgccgagatc cacgccgcct gcaaatacga tccgtggttc
      301 ctcgaagagg tccgccgcat cgtgctggcc gaggccgaga tccgcgccaa gggcctgccc
      361 gagaacgcga gcggcctctt gcggctgaag cagcagggct tcgccgatgc gcggcttgcc
      421 gagcttgcgg gatgcgaaga agcggcggtc gcggcgcgcc gtgccaagct cggcatccat
      481 cccgtctaca agcgcatcga cacctgcgcc gccgagttcc cgtccctcac gccctacatg
      541 tattcgacct atgagggcga cggcgccagc ccgcccgaat gcgaggcgga gcccagcgag
      601 cgccgcaagg tcgtgatcct gggcggcggc cccaaccgca tcggccaggg catcgagttc
      661 gactattgct gcgtccacgc cgcctacagc ctcaaggagg ctggcttcga gacgatcatg
      721 gtcaattgca atcccgagac cgtctcgacc gactacgaca cctccgaccg gctctatttc
      781 gagccgctca ccgccgagga cgtgatcgag ctggtgcgcg tcgaggagcg ccgcggcacg
      841 ctcctcggcg tcgtcgtaca gctgggcggc cagacgccgc tgaagctcgc cgccgccctc
      901 gaggcggcgc gcattcccat tctcgggacc tcgcccgacg cgatcgacct cgccgaggac
      961 cggcggcgct tccaggagct cctccaccgc ctcgacctga agcagcccga gaacggcatc
     1021 gcctcttcgg tcgaggaggc ggagaaggtc gcttcacgca tcggctatcc cttggtgatc
     1081 cggccctcct atgtgctggg gggacgcgcc atggagatcg tccacggtcc ggccggcctc
     1141 acccgctaca tgaccaacgc cgtcaaggtg tcaggcaaga acccggtgct gatcgaccgc
     1201 tatctccagg acgcgatcga ggtcgacgtc gacgcgctcg ccgatggcag cacggtcgcc
     1261 atcgccggca tcatggagca tatcgaggag gccggcatcc attcgggcga cagcgcctgc
     1321 tcactcccgc cctactcttt gccggcgccg gtcatcgccg acatcgagcg ccagacggcg
     1381 gccctcgcca aggcgctcgg cgtggtcggg ctcatgaatg tccaatacgc cgtgaaggag
     1441 cagaccgtct atgtgctcga ggtgaacccg cgcgcgtcgc gcaccgtgcc tttcgtcgcc
     1501 aaggcgaccg gcgtccccat cgccaagatc gcggcccgcg tcatggccgg cgagcgcctc
     1561 gacgcgttca agctcgaccg ctcgcggcgc cgccatgtgg cggtcaagga ggcggtcttc
     1621 cccttcgccc gcttccccgg cgtcgacctg atcctcggac ccgagatgaa gtcgaccggc
     1681 gaggtcatgg gcctcgacac cgatttcggc cgcgcttttg ccaagtcgca gctcggcagc
     1741 ggcgtcgacc tgccgctcca gggcaccgtc ttcgtctcgg tgcgcgaccg cgacaagccg
     1801 gccatggtcc agccctgccg gcgcctcgtc gaaatgggct ttacgctcct ggccacggcc
     1861 ggcaccggcg agcggctgcg cgaggcgggg ctccccgtcg aggccatcaa caaggtgctg
     1921 gaggggcggc cgcatattgt cgaccggatg ctctcgggcg gcgtccagct ggtcttcaac
     1981 accaccgagg gcgcccaggc gatcgccgac agcttcagtc tgcgccggac ggcccttacc
     2041 aacgccattc cctactacac caccgtcgcc ggcgcccggg cggcggtcga ggcgattgcc
     2101 gcgctcaggg ccggcagcct tgaagtcgct tccctgcagt cctattttgt tggatcgttc
     2161 tagccgtccc ccggcccttc gggtcgggag agggtgttat tgtcgttcca cgaaggctcg
     2221 ccgagcgggg gtggccgaaa tctctccatg aacaagatcc cgatgaccga gaccggctac
     2281 aaccggctgc aggaagagtt gaaacgcctc aaatcggtgg accgtcccgc cgtgatccgc
     2341 gccatcgccg aggcgcgcga gaatggcgac ctgtccgaga acgccgagta tcacgcggcg
     2401 cgcgagcggc aaagcttcat cgagggccgc gtcctcgagc tcgaggacaa gatcgcgcgt
     2461 gccgaggtga tcgacgtctc gaagctttcg ggcaaattgg tcaagttcgg cgccacggtg
     2521 acgctcgccg acgaggagac cgacgagaag gtgtcctatc agatcgtcgg cgaagacgaa
     2581 gccgacatca gccagcggcg gctgtcggtc acttccccgc tcgcccgcgc cctcatcggc
     2641 aaggccatcg gcgacagcgt cgaagtctcg acgccgcgcg gcgcccgctc ttacgagatc
     2701 gtcaaggtcg gcttccgctg agcggtcgag ggaccgattg ctgcttcgcc tagtcctgcg
     2761 tgcgtcgagg cggcgccagc gtatccttgc tgacgaatag caagcgccgc cggaacgccg
     2821 tgggactgtg cacagtcagc gttgcgatca ttgattcatc acccgcgaag atcggccgga
     2881 tgagattgtt gccgtgcgtc tctgaccact ccggcgtggg caagtggaag gatcgcgcga
     2941 ggtgctccgc cacggccgcg agataggcgt ctcgcacggg atcgagcggc tccggacggt
     3001 ccgcgagcgc ggcttcgcgc tcttccggtt gtccgtagaa gacctcaaga aactcgtggg
     3061 cggcgtggtc gaacgcgtca tcgtcgctgt ccctgacgcg ccgggaaatc tccaacaggg
     3121 ttctcggccg cttgctgtct atgctcatgt cgtggcaacc aatctcggta gctctcgcat
     3181 gtccgagaat acgagatcgg cgccggccgc agcgaggcgc gcgtcatggc cgggccggca
     3241 atggctcgcc cccatgaagc ccagcgccat catgccggcc gctttcgctg cggtgatgcc
     3301 gggtgcgctg tcctcgatga cgaggcagcg cgcgggatcg gccgccatcg ccctggcagc
     3361 atggaggaag agatcgggcg caggcttgcc gcgcgcgacc atcgagacgc tgaacacggc
     3421 gtcgccgaac cacttggcga ggccggtcag ccccagcgta taggcgaggc gttcgggcag
     3481 gctgctcgag gcgacgcagc gccggccggg gatcgccgcc agcgcgtcat cgacgccagc
     3541 caccgccttg agctcacgag cgaatgcctc ggcgacgcgg cgtgcggtct cggcctcgaa
     3601 gcccgccggc aatttgcgcc ccagctccgc ctcgatgagc gcgcgcccat cccgcgccgc
     3661 cttgccgacg aagcgctcca tgaagcgctc gagctcgagg tcgtagccga tttcggcgag
     3721 cgcctggacc tcgacgcggc aaacgatggt ctcgctgtcg atcagcacgc cgtcgcaatc
     3781 gaagatcagg agatcgaaag gcgagccgct agtctttggc atcgacatcg atgggcacgc
     3841 ccggctcgta tttcgaggcg cggatcgcga gcgacgaccg tacatgggca acattggggg
     3901 cggcggtgag cttcgtcgtc agaaaccgct gatagctgtc ccagtcggtg gccacgatct
     3961 tcaggagaaa atcggtctca cccgcgagca tgtgcacctc gcgcacctcg ggccaggtga
     4021 gggccaggac ctcgaaggcc ttgagatccg gctccgcctg gctcgagagg ccgacctggg
     4081 cgaagaccgt gacgccgaag cccagcgcct cgggattgac gtcggcgtgg tagccccgaa
     4141 tgaagccggc ctcctcgagc gcgcgcacgc gcctgaggca gggcggcgcc gagatgccgg
     4201 cgcgcttcgc cagatcgacg ttggtcatgc gaccggtcgc ctgcagatcg cgcagaatgc
     4261 gtcgatcgat cttgtcgagc ttgatgcggc gcatcggatc gtctctgaaa ggcgatactc
     4321 ttgcgagctg ggcagggccg ccgcgaccgc cgggaccggc gtcatgaacg gcggggcgcc
     4381 atcgtattac aaggtaataa tattgcaagc cgccctggca tcgggtatcg gcttgccagc
     4441 gcaccacacc ggggctaaag gcggccgcgc cggctcaaca tcgaggacaa aggtaggg
//
LOCUS       DASYNS010000377         9484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4153204, whole genome shotgun sequence.
ACCESSION   DASYNS010000377 DASYNS010000000
VERSION     DASYNS010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4153204"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1026
                     /locus_tag="VGP50_11750"
     CDS             229..1026
                     /locus_tag="VGP50_11750"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEV8028099.1"
                     /translation="MPAIRQYIADAFVAPGLRGNPAAVCLLEAWLPDKTMLMIAEENR
                     YSETAFVVGSGPRFRLRWFTPRVEVDLCGHATLAAAFVLSRFHGHVASDIVFETKSGD
                     LAVSTRDDLYMIDLPRRTIQSTDVSADILQAAVGARPLELAKAGPAIIAVLPSARDVE
                     LAIPRMERIEQIDCDGLIIAAQGGGADFVLRFFGPRLGLGEDPATGSALCGLAPFWSE
                     RLGKTELRARQLSPRGAELLCAVGERTVAIGGRVELFSTGDLFLGSS"
     gene            1129..1368
                     /locus_tag="VGP50_11755"
     CDS             1129..1368
                     /locus_tag="VGP50_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028100.1"
                     /translation="MENLDDRYDPDRWMASGKTCATSILAGGKRNYITQHIQNLANVL
                     RDGAPSRLRKFLKAWKELNTRVSDEELREIWRSIT"
     gene            complement(1549..3834)
                     /locus_tag="VGP50_11760"
     CDS             complement(1549..3834)
                     /locus_tag="VGP50_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV8028101.1"
                     /translation="MKTLLENYSRRGFLRGAAVTGSFVVAAQFLPARALAYATGAEAM
                     PHGVVTDPHVFVSIDKDGLVQIIAARAEMGTGAARTSLPMILADELEADWSRVKIVQS
                     PGNEEKYGNQDTDGSRSVRHFIQPMRQTGAAARLMLETAAAKRWGVAIGDVEAKNHEV
                     VNKKTGAKLGYGALAADAMALPTPAAGQIKTKDPAQFRYIGKGSIKPVDQHDIIMGKA
                     VYGIDAKVPGMKFAVIARTPVLGGKLVSYDAAETMKVPGVEKVVVLDGTPPPAKFQPL
                     GGVAVIAKNTWAAMKGRDALKTTWEDGPHGSYDSAAFKTQMEATARQPGAVLRNDGDA
                     EKALASADKVVTGEYYLPHIAHASMEPVNATANFSGGKCEVWAPVQSPGGTREEVAKN
                     LGLKPDDVVIHTTLLGGGFGRKSKCDYAIEAALLSKAVGAPVKVIWTREDDIQHDYFH
                     TVSVERIDAGLDKSGKVIAWRHRSVAPTILSIFAPGQKHEAPFEQGMGFVDNPFDIAN
                     LRCENGEADAHTRIGWFRSVSNIPHAFAVQTMVAEIAAATNRDPKEMLLELIGKPRIV
                     DPRKSPDVKDFWNYGDPFEVYPIDTARLRGVVELVAAKADWGKPLPPRHGLGIAVHRS
                     FLSYIATVVEAAVDEKGTLTVPRVWTAIDAGFVVNPERIHSQMEGSAVMGYALARYGE
                     ITFKNGRAQQSNFDSYKVTRIDESPARTQVFIVPHGIDVPASGVGEPGLPPFAPALCN
                     AIFAASGKRIRRLPIADQLSA"
     gene            complement(3839..4291)
                     /locus_tag="VGP50_11765"
     CDS             complement(3839..4291)
                     /locus_tag="VGP50_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV8028102.1"
                     /translation="MMKLSVNGEQRTFDGDPSMPLLWYLRDELGLTGTKFGCGIAACG
                     ACTVHVDGDAARACVTAMSDLDGRKVTTIEGLSTDGSHPVQKAWHALNVPQCGYCQAG
                     QIMQASALLAGNKNPSDQEINDTMAGNICRCGCYQRITAAIKAAATGA"
     gene            complement(4590..4871)
                     /locus_tag="VGP50_11770"
     CDS             complement(4590..4871)
                     /locus_tag="VGP50_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV8028103.1"
                     /translation="MAVTAARLVITGRVQGVGFRAWTRREARRRGLRGWVRNRRDGSV
                     EALLIGDAAAVDSLAEACRHGPIMARVDGVLRTPADDDGSTDFAERASV"
     gene            complement(4885..6873)
                     /locus_tag="VGP50_11775"
     CDS             complement(4885..6873)
                     /locus_tag="VGP50_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEV8028104.1"
                     /translation="MFKKILIANRGEIACRVIRTARRMGIATVAVYSDADADALHVRQ
                     ADEAVHIGPPQAAQSYLRIDRIVDACRQTGAEAVHPGYGFLSEKEAFAAALLEAGVVF
                     IGPGPAAIGAMGDKIESKKLAREAGVSTVPGHLDVIPNAEAAIRIARDIGYPVMIKAS
                     AGGGGKGMRIARSDAETRDGFRSASNEARSSFGDDRVFIEKYIEEPRHIEIQVLGDTH
                     GHVIHLGERECSIQRRHQKVIEESPSPFLDAKTRAAMGAQAVALAKAVAYSSAGTVEF
                     IVDKDRHFYFLEMNTRLQVEHPVTELVTGLDLVEWMIRIAAGETLTLKQSDVKLEGWA
                     VEARIYAEDPLRNFLPSIGRLVRYAPPAGEGIRVDTGVFEGAEISVYYDPMIAKLVGY
                     GASRAEASARLAGALDAFHIRGVSHNVSFLAAVLQRPRFAEGRLSTNYIAEEFPAGFA
                     GVTVSPEAVAEAATIAAALQRIVAESEARIAGQLAGHGRVVPEEWVVSVGDISRKVET
                     SRQGDDSVLVMHEEGADRVVTTTWQPGQALFRASLDGTPVVYQVDRDGIGFRLTHGGA
                     TLAVKVLTPFAAELLAQMPAKPVPDLSRFLLSPMPGLLVSLAVGEGQDVKAGEELAVV
                     EAMKMENVLRAERDGQVKKIRAKTGESLAVDQVILEFS"
     gene            complement(7029..8561)
                     /locus_tag="VGP50_11780"
     CDS             complement(7029..8561)
                     /locus_tag="VGP50_11780"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEV8028105.1"
                     /translation="MQDILQQLEEKRAAARQGGGKRRIAAQHAKGKLSARERLELLFD
                     PGSFEEWDMFVEHRCQDFGMADQKIPGDGVVTGYGTVNGRLVFAFSQDFTVFGGALSE
                     AHAEKICKIMDQAINVGAPVIGLNDSGGARIQEGVASLAGYAEVFQRNVLASGVVPQV
                     SLIMGPCAGGAVYSPAMTDFIFMVKDSSYMFVTGPDVVKTVTHETVTQEELGGAVTHT
                     SRSGVADLAFANDVEALLQLRRFIDFLPASNRAKPPVRPTADSPERVEPSLDTLIPAN
                     ANKPYDMRELIEKVVDEGDFFELQPSFAKNIIIGFARMEGSTVGIVANQPMVLAGCLD
                     IDSSRKAARFVRFCDCFNIPIITFVDVPGFLPGTAQEFGGIIKHGAKLLYAFAEATVP
                     KVTVITRKAYGGAYDVMSSKHIRGDVNFAWPSAEIAVMGPKGAVEIIFRGDIGDAAKI
                     EARTEEYRTKFANPFVAGSRGFIDDVIMPRSTRRRLCRSLAMLREKSLGNPWKKHDNI
                     PL"
     gene            complement(8714..9409)
                     /locus_tag="VGP50_11785"
     CDS             complement(8714..9409)
                     /locus_tag="VGP50_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HEV8028106.1"
                     /translation="MKRGYQTVGVAPAESGHGVTLDDKPLRTPAKAPVVVPSRALAEA
                     IAGEWRRQGETLRLETLPLTRLAGTAIDLVTPRRSEVIAEVARYAGTDLVCYRAGHPP
                     ELATRQHEAWQPLIDWASLRYDAPLEVTAGIVPITQAPRTLRAFAAAVGAHPPLELAA
                     LHLATATCGSLVVALALIEGRLDAEGAFAASQLDETFQIEQWGEDAEQMRRRAALKED
                     MALAERFVALLRA"
BASE COUNT         1515 a         3109 c         3191 g         1669 t
ORIGIN      
        1 cgcccgcgcc aagctggccg aagcgcaact ctgataccat caccttctcg gcacaaaaac
       61 gccccgccac tcagcggagc gcttcgctgt cggcgatgcc atgcgctact ctccgaactc
      121 gcctttttgc gccggaaagt aactaggtac aagctccgga gccgagctga acggccgcgg
      181 catcgcgctc atacggcgcg cgagcacaga attggatgag gagaggaaat gcctgcgatt
      241 cgtcaatata tcgctgatgc ctttgtagcg cccggcttgc gcggaaatcc ggcagcggtg
      301 tgcctgctcg aggcgtggtt gccggacaaa accatgctga tgatcgcgga ggagaaccgc
      361 tactccgaga cggccttcgt cgtcggtagc ggcccacgat tccgcttgcg ctggttcacc
      421 ccgcgcgttg aggtggacct ttgcggccac gcgacgctcg ctgcagcctt cgtgctgtcg
      481 cgcttccacg ggcacgtcgc ctcggacatt gtcttcgaga ccaagtcggg cgatctggcg
      541 gtcagcaccc gtgacgatct ctatatgatc gacctgcctc gccgcacgat ccaatcaacc
      601 gacgtcagcg cagacattct ccaggccgca gtgggcgcga ggccgctcga acttgcgaag
      661 gcgggccccg cgatcatcgc ggtgctgccg agcgcccgcg acgtcgagtt ggcgataccc
      721 cggatggaac ggatagagca gatcgattgc gacggcctga tcatcgcggc gcaggggggc
      781 ggcgccgatt tcgttttgcg gttcttcggt ccccgtttag gactgggtga ggatccggcc
      841 accggctcgg cactctgcgg cctagctccc ttctggagtg agcgtctcgg aaagacagaa
      901 ctccgggcac gccagttgtc tccgcgcggc gccgaactgc tctgcgcggt cggcgagcga
      961 acagtcgcca tcggcggtcg cgtcgagctc ttttcgaccg gcgacctgtt cctcggctcg
     1021 tcttagcggg cgatctgcgc cttcgaaatt ccgatcggtc ggaatgatgg ggctgtcccc
     1081 gcggttgggt gaaccctcgc ggcctcggcg ctacgggaac ttacaaccat ggaaaatctc
     1141 gacgaccgat acgaccctga ccgctggatg gcatcaggta aaacctgtgc gacttccatt
     1201 ctggcgggcg ggaagcggaa ctatattacg caacatatcc agaacttggc caacgtgttg
     1261 cgcgatggcg caccatcacg tctgagaaag tttctcaagg cgtggaaaga gctaaacaca
     1321 cgagtaagcg atgaagaact ccgcgagatc tggcggtcga tcacatagga caaagccctt
     1381 tcgggagtct acgatgactc ttgtcgcaca tctctgacga cctgctccat agggccaatg
     1441 tccggaatcg aggcggaagc agccgctata gccaccctct atggattttg acccttgctt
     1501 gggcgatatc gggcgggatc cggtcccgcc cgatatcctt ctacgtgctc aggccgacag
     1561 ctggtcggcg atgggcaagc gccggatacg cttgccggag gcggcgaaga ttgcgttgca
     1621 cagcgccggc gcgaagggcg ggaggcccgg ctcgccgacg ccgctcgccg gcacgtcgat
     1681 gccgtgcggc acgatgaaca cctgggtccg cgccggcgat tcgtcgatac gcgtgacctt
     1741 gtagctgtcg aagttgcttt gctgcgcccg tccgttcttg aaggtgatct cgccatagcg
     1801 agcgagcgca tagcccatca ccgccgagcc ttccatctgc gagtggatgc gctcgggatt
     1861 gacgacgaag ccggcgtcaa tcgccgtcca cacccggggc acggtcagcg tccctttctc
     1921 gtccaccgct gcttcgacga cggttgcgat gtagctgagg aagctgcgat ggacagcgat
     1981 tccgaggccg tgacgcggcg gcaagggctt gccccaatcc gccttcgcgg ccaccagctc
     2041 gaccacgccg cgcagccgcg ccgtgtcgat cggatagacc tcgaacggat cgccgtagtt
     2101 ccagaaatcc ttgacgtccg gcgacttgcg cggatcgacg atgcgcggct tgccgatcag
     2161 ctcgagcagc atctccttcg gatcccggtt cgtcgcggcg gcgatttccg cgaccatcgt
     2221 ctgaacggcg aaggcgtgcg gaatgttcga caccgagcgg aaccagccga tgcgggtatg
     2281 ggcgtccgcc tcgccgttct cgcagcgcag attggcgatg tcgaagggat tgtcgacgaa
     2341 ccccatgccc tgctcgaacg gcgcttcgtg cttctggccc ggcgcaaaga tcgagaggat
     2401 ggtcggagcg acgctgcgat gccgccaggc gatcactttg ccgctcttgt cgaggccggc
     2461 gtcgatccgt tccaccgaga ccgtgtggaa gtagtcgtgc tggatgtcgt cctcgcgcgt
     2521 ccagatgact ttgacgggcg ctcccaccgc tttcgacaac aaggccgctt cgatcgcata
     2581 gtcgcatttc gacttgcgcc cgaagccgcc gcccaggagt gtcgtatgga tcacgacatc
     2641 gtcgggcttg aggccgaggt tcttcgccac ctcctcgcgc gtgccgcccg ggctctgaac
     2701 cggcgcccag acttcgcatt tgccgccgga gaaattggcc gtcgcgttga ccggttccat
     2761 cgaggcgtgt gcgatgtgcg gcaggtagta ttcgcccgtc acgaccttgt ccgccgacgc
     2821 cagcgccttt tcggcatcgc cgtcattgcg caggacggcg ccgggctggc gcgcggttgc
     2881 ttccatttgc gtcttgaagg ccgccgaatc gtaggaaccg tgcggcccgt cctcccaggt
     2941 cgtcttgagg gcgtcacggc ctttcatcgc cgcccaggtg ttcttggcga tgaccgcgac
     3001 gccgcccagc ggctggaatt tggccggcgg cggcgtgccg tcgaggacca cgaccttctc
     3061 gacgcccggc accttcatcg tttcggcggc atcataggaa acgagcttgc cgcccaggac
     3121 cggggtcctg gcgatgacgg cgaacttcat gcccggcact ttggcgtcga tgccgtagac
     3181 ggctttgccc atgatgatgt cgtgctgatc gaccggcttg atcgagccct tgccgatgta
     3241 gcggaactgc gccgggtcct tggtcttgat ctggccggcg gccggcgtcg gcagcgccat
     3301 ggcgtcggcg gcaagcgcgc catagcccag ctttgcgccg gtcttcttgt tgacgacctc
     3361 gtggttcttc gcctcgacgt cgccgatggc gacaccccag cgcttggccg cggccgtttc
     3421 cagcatgagc cgcgcggcgg cgcccgtctg ccgcatcggc tgaatgaaat ggcgaacgct
     3481 gcgcgagccg tcggtgtcct ggttgccgta tttctcctca ttgcccggcg actgcacgat
     3541 cttgacgcgg gaccagtcgg cctcgagctc gtcggcgaga atcatcggca agctggtgcg
     3601 cgccgcgccg gttcccatct cggcgcgggc ggcgatgatc tggaccaacc cgtccttgtc
     3661 gatcgaaacg aacacatggg gatcggtaac gacgccgtgc ggcatcgcct ccgctccggt
     3721 tgcataggcc agcgccctgg ccggaaggaa ctgagccgcg acgacgaagc tgccggtgac
     3781 cgctgctccc ctgagaaagc cgcgccggct gtaattttct agaagggtct tcatgatgtc
     3841 acgcccccgt cgctgcagct ttgatggcgg cggtgatgcg ctgatagcac ccacatcggc
     3901 agatgttgcc ggccatcgtg tcgttgattt cctggtccga cgggttcttg ttgcccgcca
     3961 gcaaagcgct ggcctgcatg atctgccccg cctggcaata gccgcattgc ggcacgttca
     4021 gcgcgtgcca cgccttctgc acgggatggc tgccatcggt cgacaaaccc tcgatggtcg
     4081 tcaccttgcg gccgtcgagg tcgctcatgg ccgtgacgca ggcgcgggcc gcgtcgccgt
     4141 cgacatggac ggtgcatgcg ccgcatgccg cgatgccgca gccgaattta gtgcccgtca
     4201 ggccgagctc gtcgcgcagg taccagagca gcggcatcga cgggtccccg tcgaaggtgc
     4261 gttgctcgcc attgacgctt agcttcatca tcggattctc ctcgttgaga cgcatgggtc
     4321 ggccgtccag gaagggcgac cgctccatgc cttgccgtta cttcttcact gcgaagaccc
     4381 atgcgcctat cgccctgggg ggacgcgccc gcagggctgc cggcacactt gcgcggagaa
     4441 tcaccctccg ctgcggagcg ctcggacttc ctcccatgcc ctagccccgt tcattcggtt
     4501 agcgaaactc tggacccctg tattagttgt gtcaaccggc ggcaatagaa gcgaggtggt
     4561 cgtgccggat cgctggacga tccgggtctc taaacgctgg cccgctccgc gaaatcggtc
     4621 gagccgtcat cgtctgcagg cgtgcgtagg acgccgtcga cccgcgccat gatcgggccg
     4681 tggcggcagg cttcggcaag gctgtcgacg gcagcggcgt cgccgatgag gagcgcctcg
     4741 acgctgccgt cgcggcggtt ccgcacccag ccgcgcaggc cgcgccggcg cgcctcgcgc
     4801 cgcgtccagg cgcgaaatcc gacgccctgc acgcggccgg tgatgacgag gcgagcggcg
     4861 gtgacggcca tcgcccggcg cagatcaaga gaattcgaga atgacctggt cgacggcgag
     4921 gctctcgccg gtcttggcgc ggatcttctt gacctggccg tcgcgctcgg cgcgcagcac
     4981 gttctccatc ttcatcgctt cgaccaccgc gagctcttcg cctgccttca cgtcctggcc
     5041 ttcgcccacc gccagtgaga cgaggaggcc cggcatgggc gacaacagga agcgcgagag
     5101 gtcgggcacc ggttttgcgg gcatctgcgc caggagctcg gcggcgaacg gcgtcagcac
     5161 cttgacggca agcgtggcgc cgccgtgagt caagcggaag ccgatgccgt cgcggtcgac
     5221 ctgatagacg acgggggtgc cgtccagcga cgcgcggaag agcgcttggc ccggctgcca
     5281 cgtcgtcgtg acgacacggt ccgcgccctc ctcatgcatg acgaggacgc tgtcgtcgcc
     5341 ttggcgcgac gtctccacct tgcggctgat atcgccgaca gagaccaccc attcctcggg
     5401 gacgacgcgg ccgtggccgg cgagctggcc cgcgatgcgc gcttcgcttt cggcgacgat
     5461 ccgctgcaaa gcggcggcga tggtcgcggc ttccgccacg gcctcgggcg agaccgtcac
     5521 gccggcgaag cccgccggaa attcctcggc gatgtaattg gtcgagaggc ggccctcggc
     5581 gaagcgcggg cgctggagca ccgcggcgag gaacgagacg ttgtgcgaga cgccgcggat
     5641 gtggaaggcg tcgagcgcgc cggccaggcg ggcgctcgcc tcggcgcgcg atgccccgta
     5701 gcccaccagc ttggcgatca tcgggtcgta gtagacgctg atctcggcgc cctcgaaaac
     5761 gccggtgtcg acgcggatgc cctcgcccgc gggcggcgca tagcgcacga ggcggccgat
     5821 cgagggcagg aagttgcgca ggggatcttc ggcataaatg cgcgcctcga ccgcccagcc
     5881 ctcgagcttg acgtccgact gcttgagcgt gagcgtctcg ccggcggcga tgcggatcat
     5941 ccattcgacg agatcgaggc cggtgacgag ctcggtcacg ggatgctcga cttggagccg
     6001 cgtgttcatc tcgaggaaat agaagtgccg gtccttgtcg acgatgaact cgaccgtccc
     6061 ggctgacgag taggcgaccg ccttggcgag cgccaccgcc tgcgcaccca tggcggcgcg
     6121 cgtcttggcg tcgaggaagg ggctcggcga ctcctcgatg accttctgat gccggcgctg
     6181 gatcgagcat tcgcgctcgc cgagatggat cacatggcca tgggtgtcgc ccagcacctg
     6241 gatctcgata tggcgcggct cctcgatgta cttctcgatg aagacgcggt cgtcgccgaa
     6301 gctcgaccgt gcctcgttgc tggcggagcg gaagccgtcg cgcgtttcgg cgtcgctgcg
     6361 cgcgatgcgc atgcccttgc cgccgccgcc cgccgaggcc ttgatcatca cgggatagcc
     6421 gatgtcgcgg gcgatgcgga ttgcggcctc ggcattggga atgacgtcga gatgtcccgg
     6481 cacggtcgag acgccggcct cgcgggcgag cttcttcgat tcgatcttgt cgcccatggc
     6541 gccgatcgcg gccgggcccg gaccgatgaa gacgaccccg gcctccagca aggcggcggc
     6601 aaaagcttcc ttctccgaga ggaagccgta gccgggatgc accgcctcgg cgccggtctg
     6661 gcggcacgcg tcgacgatgc ggtcgatcct aagataactc tgggccgctt gcggcgggcc
     6721 gatgtgcacg gcctcgtcgg cctggcgcac atggagggcg tcggcatcgg cgtcggaata
     6781 gacggcgacc gtggcaatcc ccatgcgccg cgccgtgcgg atcacccggc aggcgatctc
     6841 gccgcgattg gcaatgagga tcttcttgaa catgcgcgcc tagccgttgc ggcgaagaaa
     6901 agatggacca cagaggcagt gagacagtga gaagagagga atggcgcgcg atgcgcgcca
     6961 agactcggtc tctctgtgcc tcactgtctc tgtggtgaat ccttcttttc gcgacatgcg
     7021 tcaatgcctc agagcgggat gttgtcgtgc ttcttccagg gattgccgag cgacttctcg
     7081 cgcagcatgg cgagcgagcg gcagaggcgg cggcgggtgc tgcgcggcat gatcacgtcg
     7141 tcgatgaagc cgcggctgcc ggcgacgaag ggattggcga atttcgtgcg gtactcctcg
     7201 gtgcgcgcct cgatcttggc ggcatcgccg atgtcgccgc ggaagatgat ctcgaccgcg
     7261 cctttggggc ccatgacggc gatctccgcc gagggccagg caaagttgac gtcgccgcgg
     7321 atgtgcttcg agctcatgac gtcataagcg ccgccatagg ccttgcgcgt gatcaccgtc
     7381 accttgggca cggtcgcttc ggcgaaggcg tagagcagct tggcgccgtg cttgatgatc
     7441 ccgccaaatt cctgcgccgt gccgggcagg aatcccggca cgtcgacgaa ggtgatgatg
     7501 gggatgttga agcagtcgca gaagcgcacg aagcgcgccg ccttgcgcga gctgtcgatg
     7561 tcgaggcagc cggccagcac catcggctgg ttggcgacga tgccgacggt cgagccttcc
     7621 atgcgcgcga agccgatgat gatgttcttg gcgaagctcg gctgcagctc gaagaagtcg
     7681 ccctcgtcga cgactttctc gatcagctcg cgcatgtcat agggcttgtt ggcattggcc
     7741 gggatcagcg tgtcgagcga gggctcgacg cgctcgggcg aatcggcggt tgggcgcacc
     7801 ggcggcttgg cgcggttgga cgccggcagg aaatcgatga agcggcgcag ctgcagcagc
     7861 gcttcgacgt cgttggcaaa ggccagatcg gcgacgccgg agcggctcgt gtgggtgacg
     7921 gcgccgccca actcctcctg cgtcaccgtc tcgtgggtca ccgtcttcac cacgtcgggg
     7981 ccggtgacga acatgtagga gctgtccttg accatgaaga tgaagtcggt catcgccggc
     8041 gagtagacgg cgccgccggc gcagggaccc atgatcagcg acacttgcgg cacgacgccc
     8101 gaggccagca cgttcctctg gaacacctcg gcataaccgg ccagggacgc gacgccttcc
     8161 tggatgcgcg cgccgccgga atcattgagc ccgatcaccg gcgcgccgac gttgatcgcc
     8221 tggtccatga tcttgcagat cttctcggca tgggcctcgg acagcgcgcc gccgaagacg
     8281 gtgaagtcct ggctgaaggc gaagacgagg cggccgttga ccgtgccata gccggtgacg
     8341 acgccgtcgc ccgggatctt ctggtcggcc atgccgaaat cctggcagcg atgctcgacg
     8401 aacatgtccc actcctcgaa ggagccggga tcgaagagca gctcgagccg ctcgcgcgcg
     8461 ctgagcttgc ccttggcgtg ctgggcggcg atgcggcgct taccgccgcc ttgccgtgcc
     8521 gcggcgcgct tctcctcgag ctgctggagg atgtcctgca tgtcgcctct gctccctgcc
     8581 gcccaaccgg cgcgtgtggg gagatatcac caaccggccg ctattgccag agccggggac
     8641 ggcgcggccc cgccggaact ccaatgcatg ctttgcgcca ccatcctctt tctcccgcaa
     8701 ggggagaggg gaatcaggcc cgcagcaagg cgacgaagcg ctcggccagc gccatgtcct
     8761 ccttgagcgc ggcgcggcgg cgcatctgct ccgcgtcctc gccccattgc tcgatctgga
     8821 aggtttcgtc gagctgcgag gcggcaaagg cgccctcggc gtcgagccgc ccctcgatca
     8881 gcgccagcgc gacgaccagc gacccgcacg tcgcggtcgc gagatggagc gcggcgagct
     8941 cgagcggcgg gtgcgcgccg accgcggcgg cgaaggcgcg caaggtgcgc ggcgcctgag
     9001 tgatgggcac gatccctgcg gtgacctcaa gcggcgcgtc atagcgcagg ctcgcccaat
     9061 cgatgagcgg ctgccaggcc tcgtgctggc gcgtggcgag ctccggcggg tggccggcgc
     9121 gatagcagac gaggtcggtg ccggcatagc gggccacctc ggcgatgacc tcggaccggc
     9181 gcggcgtcac gaggtcgatg gccgtgccgg cgagccgggt caaaggcagc gtctcgagcc
     9241 gcagcgtctc cccttggcgg cgccactccc cggcgatcgc ctcggccagc gcgcggctcg
     9301 gcacgacgac cggcgccttg gccggcgtgc gcaacggctt gtcgtcgagg gtcacgccat
     9361 ggccggactc cgccggcgcg acgcccacgg tctgatagcc gcgtttcacg gcaccggcgc
     9421 tagatcggcg aagggatcgg gctcatcggc ttcggcgaag ccgaagaagg cccaggtctc
     9481 cttc
//
LOCUS       DASYNS010000378        16006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1342648, whole genome shotgun sequence.
ACCESSION   DASYNS010000378 DASYNS010000000
VERSION     DASYNS010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 16006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16006
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1342648"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VGP50_11790"
     CDS             <1..225
                     /locus_tag="VGP50_11790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV8028107.1"
                     /translation="AALPKLDAPAHTPLAAIAGMPPDPTRLPPGCSFNPRCPPSRERC
                     RRDAPPLADPDGGGHAYACWYPLPARVAAE"
     gene            complement(306..803)
                     /locus_tag="VGP50_11795"
     CDS             complement(306..803)
                     /locus_tag="VGP50_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028108.1"
                     /translation="MQHVLVVDEPSAGAEIKTLLTPRYRVSLAEDGERAVELLDRDGP
                     DLVLLEAALPGMSGIELAAYVVQRGLPAVVISPKPAICAGLDRLGWPHLRKPFEVDAL
                     LHTVQQTLALAQQRQHVVRQSLRVFARTSGELREAILNLSELRQRVQETLARSRRQPG
                     PREPH"
     gene            complement(845..1258)
                     /locus_tag="VGP50_11800"
     CDS             complement(845..1258)
                     /locus_tag="VGP50_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028109.1"
                     /translation="MGELASPSIAGLKILLVEDESLVAMLVEDALDALQCQVIGSASR
                     MRNARELAGRDGFDLAILDINIGGEPVYPVAEVLARREIPFVFLTGYGVAGVAEPYRG
                     RPILQKPFQLEELRKLLVQALRLRDNARSEERRRH"
     gene            complement(1307..1432)
                     /locus_tag="VGP50_11805"
     CDS             complement(1307..1432)
                     /locus_tag="VGP50_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028110.1"
                     /translation="MTNRDDVTVSAGTECRSMLSQIAQRRRRHSDEYQEQIQAIR"
     gene            complement(1542..5120)
                     /locus_tag="VGP50_11810"
     CDS             complement(1542..5120)
                     /locus_tag="VGP50_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEV8028111.1"
                     /translation="MAARSSKRKSRKPARKSSRRRAASPRPPAASTTISPATCPVVAL
                     GASAGGLEAFEIFFRRMPPDSGIGFVLVPHLDARHKSAMTELVQRYTRMNVVEITDGM
                     PVIPDRVHIIPPNAGLSIHGGHFAVVTPREEPMTIDPFLKSLAEDQGDNAVAVILSGT
                     GSDGALGVKAIKEHGGVAMAQTGRSLRYDSMPHSAVATGLIDFELPVEDMPAKLVEYA
                     SHLRQSQGGREADNFRTEARRHLAKIYMLLRSKTGHDFSRYKDSTFLRRLQRRMQITQ
                     IAKVAAYASLLRKDPREINLLFRELLIGVTHFFRDPRAFEVLDKEVIAKLVAGNKADG
                     VIRVWVAGCSTGEEAYSIAVLLRERLAQSAAPPKAQIFATDIDDHALDIARAGRYSEA
                     IAQDISPERLERFFIRDGNTYRVAKELREMCIFSVHNVISDAPFSRLDLVACRNLLIY
                     LDQSLQNRVIPLFHFALQQGGYLFLGPSENVTHQSKLFTKVDARFRIFKARPARLERP
                     PLEFGISAGSHRNPAAMQRATAASSEETVSRRAMRVMETYAPAYIVVDDRHEVLHFAG
                     RTGKYLQPTPGTATLDIFSILETSLRPDVRTALHRAAATGQRAVQENAYLMANGNAQP
                     VNIIVEPIPAAENGQHYFVVAFQELGGVRAREPGTPQPTDDGARNETIANLEGELLAT
                     RERLQATIEELETSNEEMKASNEEFQAVNEELQSSNEELETSKEELQSVNEELETVNS
                     ELNMKIENLERAMNDRKNLLESTQIATIFLDSELRIKSFTPAITDIFHLIDSDYGRPV
                     THITSRLAYGDLEHDVRRVLRTLTRAEQEVALADGSAVYMMRILPYRTVDNVIDGVVI
                     TFIDLTELKRGAAGLARLAAIVENSQDAIIGMAPDGTITNWNAGAERIYGHRAAEAVG
                     RPLSLVMPAERANELHAIVERLKRSRIAALTETERVAKDSRHLKVLSSVSPVRDSGGK
                     LVAASVIEHDITGRKHAEEHQKMLLAELNHRVKNALTAVQSIAARSLRNSASLEEFRV
                     TFEGRLQALAKAHDLLAASRWQGADLGDIVLTELAPYGEPERVQVRGDKFMLTSNASL
                     ILGMAFHELAVNAVKHGAFSGDQGRLEVSWDKTADGRLVVQWIEQSGPAIKPGPRKSG
                     FGLKLIERGVREDLQGKVTVAFPKTGLRCVLDIPIAEAQESPAGTE"
     gene            complement(5330..5602)
                     /locus_tag="VGP50_11815"
     CDS             complement(5330..5602)
                     /locus_tag="VGP50_11815"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEV8028112.1"
                     /translation="MFTLHEGRRYRLKFRNGSDDIHPLHLHRHSFELTRIAGKPTAGV
                     IKDVVTLGGFQELEFDFVADNPGATLFHCHQQLHMDFGFMALFDYA"
     gene            complement(5801..5962)
                     /locus_tag="VGP50_11820"
     CDS             complement(5801..5962)
                     /locus_tag="VGP50_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028113.1"
                     /translation="MFSVDDPYEDCSIAAAFIETAPISEAARAKVCHGNAERRLGLAT
                     GAAERISAR"
     gene            complement(6048..6395)
                     /locus_tag="VGP50_11825"
     CDS             complement(6048..6395)
                     /locus_tag="VGP50_11825"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEV8028114.1"
                     /translation="MKPVERRPARERKAEISNPVANPYGALLEGNQQAFTRWLQGMFG
                     VFQEISRFTQARMQEDMAAWSALTSCRTPEEAMECQRRFAAKAAEQFSEEMNRLSQLM
                     MKMAVPTLPPPRQ"
     gene            6695..7987
                     /locus_tag="VGP50_11830"
     CDS             6695..7987
                     /locus_tag="VGP50_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEV8028115.1"
                     /translation="MPTSSKHAETVVLHAGYRSDPSTGAVAVPIYQTTSYQFRDTQHA
                     AALFGLAELGNIYTRIMNPTTAVLEERLAALEGGVAALALSSGQSATMMAIQNICHSG
                     DNFVSATDLYGGTWNLFLNTLSTMGIEVRFVDPADPEGFRRATDERTRCYYAETLPNP
                     KLEVFPIAEVARIGRELGVPLIMDNTAAPVLCRPLDHGAAIVMHSTTKYIGGHGTSIG
                     GIVIDGGNFDWEANAKRFPMLNTPDPSYHGAVWTQAVKPLGPIAYILKMRVTLLRDLG
                     AAMSPFNAFLFLQGLETLPLRIRAHCANASRVAKYLSEHSKVSRVIFPGAMEGRAKRR
                     ADTYLRGGYGGLVGFELAGGMESGRRFIDALKMFYHVANIGDARSLAIHPASTTHSQL
                     SPEEQLQTGVSERYVRLSLGIEHEEDILADLSQALDAA"
     gene            8278..9336
                     /locus_tag="VGP50_11835"
     CDS             8278..9336
                     /locus_tag="VGP50_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028116.1"
                     /translation="MPLYESYTTTVVGAHSVPRWYEALDRLVTFGQLASGDFEDAQLR
                     CSQAAILEQELAGIDVITGGEMHRRRHNRNSPPNAMLNYFWQKIPAFQGETRPKPITR
                     HDPNVFHPAAICRGPIAEDIDLGLVEEFKTVSAFAHKPVKVTMTGPHLLAAVAYDEYY
                     NDLPRMMSDLGKLLHRNFKLLAAAGCKHIQIDEPYFTAAEDDEVRAAVEVINMAIEGL
                     PEDVHVLVHICQGNYAMGADYDGQIGHRYFDMGRYKADLICRIECDAYLIEHDMTHHY
                     EGLLGDRQLGVGAVDVQSPNVETGELVADRIGAYRWLAPEQTIITSSCGLNHLPRHVA
                     LGKLRAMTEAKAILQRRR"
     gene            9349..11373
                     /locus_tag="VGP50_11840"
     CDS             9349..11373
                     /locus_tag="VGP50_11840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8028117.1"
                     /translation="MAQNQDDGGSLEARLRATEERLRLAEAASGIAMFDLDPASGEGE
                     RTAQLAALFGIDQQQETSSFADRVKAVFADDVPKLRAALEIAGRTGTYQVEFRVRHAD
                     GSVHWVAGHGALVKDEVGRARLCGAYYEITARKMLEARLLALNETLEARVTERARQLA
                     ANATQLEETERRFRLLVDAVTDYAIFMLDPEGNIVSWNPGAQRIKGYDRDEIVGSHFS
                     RFYTEQDRASGVPGRALATATRTGKFEAEGWRVRKDGSTFWASVVINAIHGRDGSLLG
                     FAKVTRDLTERRSSEERLHQAQKMEAVGQLTGGVAHDFNNLLTVITGNIDSLQRHLPE
                     TADPNLRRFANSALRGAERAALLVHRLLAFSRRQPLAPKSVSVNTLIAGMSELLRRTL
                     GESVAIETVLGGGLWLAFIDANQLESALLNLALNARDAMPDGGKLTIEAANVFLDEAY
                     AANAEILPGQYVGLFVSDTGIGMTPEVAAHAIDPFFTTKEPGQGTGLGLSQVYGFIKQ
                     SGGHLKIYTEAGAGTTVKLYLPRHLASQAETQAVSAAPAIPRGRGEAILVVEDDADVR
                     SFTVDMLRELEYRVIEAHDGASGLRQLDAHRDVALLFTDVGLPGGINGRQLADEARRR
                     RPGLKVLFTSGYARNAIVHHGRLDPGVELLQKPFAFAALAGRVRTALDAR"
     gene            11625..11804
                     /locus_tag="VGP50_11845"
                     /pseudo
     CDS             11625..11804
                     /locus_tag="VGP50_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384943.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
     gene            complement(11824..12255)
                     /locus_tag="VGP50_11850"
     CDS             complement(11824..12255)
                     /locus_tag="VGP50_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028118.1"
                     /translation="MPIPNPEPGLVISYAYLWHHEHQAGREEGRKDRPSVSLLAVERE
                     ADGTTMVTVLPITHGRPADPASAVEIPSAVKRHLGMDDDASWIVVAEGNEFLWPGYDL
                     RKLPHSDRYDYGFVPPRFFKQVLDAFVAYYGTGKRRLAARD"
     gene            complement(12265..12546)
                     /locus_tag="VGP50_11855"
     CDS             complement(12265..12546)
                     /locus_tag="VGP50_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028119.1"
                     /translation="MTIPKIVPASEFTRNFGRYRMRAQREAVAVSSHGQITGYFVGPD
                     EYEEFRRFRESRRSFATADLSEEKVKAIGASRMDARHASLDTMLDPKSK"
     gene            complement(12803..14503)
                     /locus_tag="VGP50_11860"
     CDS             complement(12803..14503)
                     /locus_tag="VGP50_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028120.1"
                     /translation="MTQEPVNILLVDDQPAKLLSYEAILDDLGENILIAGSAQEALEH
                     LLKKNVAVMLVDVRMPEVDGFELATMVRNHPRFETTAIIFVSAVALTDLDRLKGYEHG
                     AVDYVPVPVVPELLRAKVRIFAELYRKSKQLRELNAELERRVAERTAELAQTNADLER
                     RVEERTREREAAIAQVHEMQKLESLGQLTGGVAHDFNNVLMAVLGNLDLLRTLPNDSS
                     AKRLIEGAIRAAERGATLTKRMLAFARRQELRPEAVDVARLINGMGEMLRRSLGPTID
                     IGMAFAEDLALVRVDPNQLELAVLNLALNGRDAMPQGGRLTVGARREEVVSGSVQGPG
                     PGEYIVIVVTDGGTGMDETTLKRAAEPFFTTKGVGKGTGLGLSMVYGMATQSEGAVRI
                     ASRVGVGTTVELWLPVADSTEIHAPQAATAAVQSPVPPRRVLLVDDDVEVTTATVAML
                     EQLGHRVVVASSGAGALELLQAAVPVDLVITDHAMPGMTGSDLADRIRETWPGLPIIL
                     ATGYADVPSSNDQDMARLDKPYRLENLSFAIATVVTKRTGDAANAGSAPASERITNYG
                     "
     gene            complement(14519..>16006)
                     /locus_tag="VGP50_11865"
     CDS             complement(14519..>16006)
                     /locus_tag="VGP50_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028121.1"
                     /translation="EKQKIIFEAFQQADASTSRKYGGTGLGLAISRELANLLGGEIQL
                     RSTPGMGSTFTLYLPTKYAGPTSPQRVAQTAASSVAAVTGARLAEAPPEAIPDDRASI
                     QPGETTLLIVEDDPHYARIMADLAHDSGFKVLVAARGGDALVLAREYQPSAVSLDVFL
                     PDMLGWTVLSQLKQNPTTRHIPVQILTLDEDRQHGLARGAFSFITKPTNKEGLEQALT
                     RIKEYTSRRRRRLLVVEDNAAERLSITELLGHDDIEIVSVESGSEALEALRQGSIDCV
                     VLDLRLPDMSGFQLLEQIRDDVGLADVPVVVFTGRELSPEEDAQLHTMARSVVVKGVE
                     SPERLLDETALFLHRVVSDLPAEKQSLLERLHSSDEDLVGKTVLLVDDDARNIFALSS
                     VLERRGMDVLTATTGSEAIALLESTPNVAIVLMDIMMPEMDGYETMQVIRTKSGFRRL
                     PIIALTAKAMKGDREKCLEAGASDYLAKPVNTEQLLSALRMWLHR"
BASE COUNT         2630 a         5199 c         5352 g         2825 t
ORIGIN      
        1 gcggcgctgc cgaagctcga tgcgccggcg catacgccgc tcgcggccat tgccggcatg
       61 ccgcccgatc cgacgcggct gccgcccggc tgctccttca atccgcgctg cccgccgagc
      121 cgcgagcgct gtcgccgcga cgcgccgccg ctcgccgatc ccgacggcgg cggtcatgcc
      181 tatgcgtgct ggtaccctct gcccgcgcgc gtcgcggcgg agtgagaggc gagagggatt
      241 aaggattttg cgaagcagga tgtgcggatg ctatcgccgc ggcgcggacg ccggcatgca
      301 cgcgcctaat gcggctcgcg cgggcccggt tggcgacgcg agcgcgccag cgtctcctgc
      361 acacgctggc gcaactcgga caagttcagg atcgcctcgc gcagctctcc cgatgtccgg
      421 gcgaagaccc gaagggactg gcggacgacg tgctgtcgct gctgcgccag cgccagggtc
      481 tgttggaccg tgtggagaag cgcgtcgacc tcgaagggct tgcgcagatg aggccagccg
      541 agccggtcga gcccggcgca gattgcgggc ttcggcgaaa tgacgaccgc gggcaacccg
      601 cgctgcacca catacgcggc cagctcgatg ccggacattc ccggcaacgc cgcttcgagg
      661 aggacgagat cggggccatc gcggtcgagc agctcgaccg cacgctcgcc gtcctctgcc
      721 aggctcacgc gataccttgg cgtcaggagc gtctttatct cggcgccggc gctcggctcg
      781 tcgacgacca gaacatgctg catcgcaatg ctccctcgcc tcgcggcgag cgctatgagc
      841 cggctcagtg gcgccggcgc tcctcgctcc gggcattgtc gcgcaagcgc agggcttgta
      901 ccagaagctt gcgcagctcc tcgagctgga acggcttctg caggatcggg cggccacggt
      961 agggctcggc cacgcccgcc acgccatagc ctgtcaggaa gacgaaaggg atctcgcggc
     1021 gcgccaggac ttcggcgacg ggatagaccg gctcgccgcc gatattgatg tcgaggatcg
     1081 cgaggtcgaa cccgtcgcgc ccggcgagct cgcgcgcgtt gcgcatgcgc gaggcggagc
     1141 cgatcacctg gcattgcaac gcgtcgagcg cgtcctccac cagcatggcc accagcgact
     1201 cgtcctcgac gagcagaatc ttcagcccgg cgatgctcgg gctcgctagc tctcccatgc
     1261 gccgctcact cgcgaaaaat cgttctcgag gtgcgtaacc gagacctcag cggatggcct
     1321 ggatctgctc ttggtattcg tcactgtgtc gccgtcggcg ttgcgcgatc tgcgagagca
     1381 tgctgcggca ttcggtgccg gcactgaccg tgacgtcgtc gcgattggtc acggccgctc
     1441 ttgcggcgta aacgacggcg tgaatggccg cgttccgaaa acgatgcggc gaaaagccgc
     1501 ggtccgaccc tggtgcgtgt gcccgtcccc gggcatcgcg atcactcggt gcccgcgggg
     1561 ctttcttgcg cttcggcgat gggaatgtcg aggacgcagc gcagtcccgt cttcgggaat
     1621 gcgacggtca ccttgccctg caggtcttcc ctcacaccgc gctcgatcag cttgaggccg
     1681 aagccgctct tgcgcggacc cggcttgatc gcgggtccgc tttgctcgat ccactggacg
     1741 accagccgtc cgtcggctgt cttgtcccag ctcacctcca gccggccctg gtcgccggag
     1801 aaggcgccgt gcttgacggc gttcacggcg agctcgtgga aggccatgcc gaggatgagg
     1861 gaggcgttcg acgtgagcat gaacttgtcg ccgcgcacct ggacgcgttc cggctcgcca
     1921 taaggagcga gctccgtgag cacgatgtcg ccgaggtcgg cgccctgcca gcggctcgcg
     1981 gccagcaggt catgggcctt ggcgagcgct tggagacgtc cttcgaaggt aacgcgaaat
     2041 tcctcgagcg acgcgctgtt gcgcaggctg cgggcggcga tcgactgcac cgccgtgagc
     2101 gcgttcttga cgcgatgatt gagctcggcc agcagcatct tctggtgctc ttcggcgtgc
     2161 ttgcggccgg tgatgtcatg ctcgatcacg gaggcagcga cgagcttgcc gccggaatcg
     2221 cggaccggcg acacgctcga cagcaccttg agatgccggc tgtccttggc gacgcgctcg
     2281 gtctcggtca gcgccgcgat ccgcgaccgc ttcaggcgct cgacgatcgc gtgaagctcg
     2341 ttggcccgct ccgccggcat caccagcgac aagggccggc cgaccgcctc cgcggcgcga
     2401 tggccgtaga tccgctcggc gccggcgttc cagttcgtga tcgtgccgtc gggcgccatg
     2461 ccgatgatgg cgtcctgcga gttctcgacg atggccgcaa gccgcgccag gcctgccgcg
     2521 ccacgcttca gctcggtcag gtcgatgaac gtgatcacga cgccgtcgat gacattgtcg
     2581 acggtgcgat agggcaggat gcgcatcata tagacggcgg agccgtcggc gagcgccact
     2641 tcctgctcgg cccgcgtcag cgtgcgcagg acgcggcgca cgtcgtgctc gaggtcgcca
     2701 taggcgaggc ggctcgtgat atgcgtgacg ggccgcccgt agtcgctgtc gatcaggtgg
     2761 aagatgtcgg tgatcgccgg cgtgaagctc ttgatgcgga gttcgctgtc gagaaagatc
     2821 gtcgcgatct gcgtgctctc gagcaggttc ttgcggtcgt tcatcgcccg ctcgaggttc
     2881 tcgatcttca tgttgagctc gctgttgacg gtctcgagct cctcgttcac ggattgcagc
     2941 tcttccttcg acgtctcgag ttcctcgttg gaggattgca gctcttcgtt gacggcctgg
     3001 aactcctcgt tcgaggcctt catctcctcg ttcgaggtct cgagctcctc gatggtcgcc
     3061 tgaaggcgct cgcgcgtcgc cagcagctcg ccctcgagat tggcgatggt ctcgttccgg
     3121 gcgccgtcgt cggttggctg cggggtcccg ggctcgcgcg cacgcacgcc gccgagctcc
     3181 tgaaaggcga cgacgaagta atgctgcccg ttttcggcgg cgggaatggg ttcgacgatg
     3241 atgttgaccg gctgggcgtt gccgttcgcc atgagatagg cgttctcctg gaccgcgcgc
     3301 tgccccgtcg ccgccgcccg atgcaaggcc gtgcggacgt cggggcggag actggtctcg
     3361 aggatgctga agatgtcgag cgtcgccgtg cccggcgtcg gctgaaggta ctttccggtg
     3421 cggcccgcga aatgcaacac ctcgtgacgg tcgtcgacga cgatataggc cggcgcataa
     3481 gtctccatga cgcgcatggc gcggcgactg acggtttcct ccgacgaagc cgcggtcgcc
     3541 ctttgcatgg cggcaggatt gcgatgggac cccgcggaga tgccgaattc gagcggcggg
     3601 cgctcgaggc gggcaggccg cgccttgaag atgcggaaac gcgcatcgac cttcgtgaac
     3661 agcttgcttt ggtgggtgac gttctcggac ggtccgagga agagatagcc gccctgctgc
     3721 aacgcgaagt gaaagagcgg aatgacgcga ttctgcaagg attggtcgag atagatgagc
     3781 agattgcggc aggcgacgag atcgagccgc gagaagggcg cgtcgctgat gacgttgtgc
     3841 accgagaaga tgcacatctc gcggagctcc ttggcgacgc gataggtgtt gccgtcgcgg
     3901 atgaagaagc gctcgaggcg ctccggcgag atgtcctggg cgatggcctc ggagtagcgc
     3961 ccggcgcggg cgatatcgag ggcgtggtcg tcgatgtcgg tggcgaagat ctgcgccttg
     4021 ggcggagccg ccgactgcgc cagccgttcg cgcagcagga cggcgatgga ataggcttcc
     4081 tcgcccgtcg agcagcccgc gacccagacg cggatgacgc cgtccgcttt gttcccggcg
     4141 acgagcttcg cgatcacctc tttgtcgagg acctcgaagg cacgtggatc gcggaagaag
     4201 tgggtgacgc cgatcagcag ctcgcggaac aggagattga tctcgcgcgg gtccttgcgc
     4261 aacaggctgg cataggccgc gaccttggcg atctgcgtga tctgcatgcg ccgctgcagc
     4321 cgccgcagga acgtgctgtc cttgtagcgg ctgaaatcat ggcccgtctt ggaacggagc
     4381 agcatgtaga tcttggcgag atgccggcgc gcctccgtgc gaaaattgtc cgcttcccgc
     4441 ccgccctgcg actggcgcag gtgactggcg tattcgacga gcttcgccgg catgtcctcg
     4501 accggcagct cgaaatcgat gaggccggtc gcgacggcgc tgtgcggcat gctgtcatag
     4561 cgcagcgacc gcccggtctg ggccatcgcc acgccgccat gctccttgat cgccttgacg
     4621 ccgagcgcgc cgtcgctgcc tgtgccggac aggatcacgg cgacggcgtt gtcgccctga
     4681 tcctcggcga gcgacttcag aaacgggtcg atggtcatgg gctcttcgcg cggcgtgacg
     4741 accgcaaagt ggccgccatg gatgctcagc ccggcattgg gcggaatgat atggacccgg
     4801 tccgggatga ccggcatccc atcggtgatt tccaccacgt tcatccgcgt gtagcgctgc
     4861 accagctcgg tcatcgcgct tttgtggcgc gcgtcgagat gcgggaccag cacgaagccg
     4921 atgccgctgt ccggcggcat gcgcctgaag aagatctcga aggcttcgag gccgccggcg
     4981 gacgcaccca gcgcgacgac gggacatgtg gcgggagaaa tcgtggtaga ggccgcgggc
     5041 gggcgcggcg aggcggcccg ccgccggctg ctcttccggg ccggcttgcg ggacttccgt
     5101 ttcgacgatc gggccgccat gaggcgcctc gcacctcccc gtgtgacccc actgtagcgc
     5161 ctcgcgccgc gcgcttgcca gcggcgattc ggccgtctgc ctcagaaaga accggcagca
     5221 ccgggtagat catctccgtc gagacgtcgg gagggagctg gcgaaggcgg gaagattaag
     5281 tgttccgcgc gagccggcgg aatatcgccc gatcgccggc cgcgacgcat caggcataat
     5341 cgaagagcgc catgaagccg aaatccatgt gcagttgctg gtgacagtga aagagcgtcg
     5401 ctccggggtt gtcggcgacg aaatcgaatt cgagctcctg gaacccgccc agcgtgacca
     5461 catccttgat cacgccagcg gtcggcttgc cggcgatgcg cgtgagctcg aaactgtggc
     5521 ggtgcagatg cagcgggtgg atgtcgtcgc tgccattgcg gaatttgagg cggtagcgcc
     5581 gcccctcatg cagcgtgaac atcggccgcc gcgtctcctc atcggcggcc agcagcgagc
     5641 gctgtgcggc accgacaaag caaaggcgcc ggcgcctttc aggagcaagc ggcggtcgat
     5701 catgaccgcg ttccgccgtg ggtgagggtg ctcgtcatcc accgccttcg tcgccttcga
     5761 cttttgcggt gtctcgagaa aatcccgtcg atgggaaact ctaccgggcc gaaatccgct
     5821 ccgccgcgcc ggtggcgaga cccaaccgcc gctcggcgtt gccgtggcag accttggcgc
     5881 gcgccgcttc gctgatcggc gcggtttcga tgaacgcggc ggcgatgctg caatcctcat
     5941 agggatcgtc gaccgagaac atgacgctgt cctcgccgag aagagggcgt gatgcgtcaa
     6001 ccgcgcgcgg gcgcatgttc ctcgaccgcg acgtcaacac cgaggcgcta ttgccgtggc
     6061 ggaggcagcg tcgggaccgc catcttcatc atcagctgag acagccggtt catctcttcg
     6121 ctgaactgct ccgccgcctt cgcggcaaag cgtcgctggc attccatcgc ctcttcggga
     6181 gtgcggcagc ttgtcaaagc cgaccacgcg gccatgtctt cctgcatgcg cgcttgcgtg
     6241 aagcgcgaga tctcctggaa gactccgaac atgccctgga gccagcgggt aaaggcttgc
     6301 tgattgccct cgagcagggc gccgtacggg ttcgcgacgg ggttgctgat ctccgccttg
     6361 cgctcccgcg ccgggcgccg ctcgaccggc ttcacctcgg cagttttcat ttcgtccggc
     6421 atgatcggtc ctcccggttt tcgagatgtc gaaatagacc atcggccgcc atatcgggca
     6481 ttgatctagc tcaagcgcgg cggccgcgca aatggcggca aggcggcgcc gaaccgccgc
     6541 gcggcgggca tggaggagga ggattgggcg cgcgaagcgt gccactcttc tcttctccgt
     6601 gacctccgtg tctccgtggt gaatactggg caacggattg cttcctcgga agcgaggtac
     6661 taggacaacg agcagcgaaa acgagggaaa ccccatgccg acttccagca agcacgccga
     6721 gaccgtggtg ctccacgccg gctaccggtc cgatccgtcg accggtgcgg ttgccgttcc
     6781 gatctaccag acgacgagct accagttccg cgacacgcag cacgccgccg cgctcttcgg
     6841 cctcgccgag ctcggcaaca tctatacgcg gatcatgaat ccgacgaccg ccgtgctcga
     6901 ggagcggctt gccgcgctcg agggcggggt cgccgcgctg gcgctctcgt cggggcagtc
     6961 ggcgaccatg atggcgatcc agaacatctg ccactcgggc gacaattttg tctccgcgac
     7021 cgacctctat ggcggcacct ggaacctgtt cctcaatacg ctcagcacca tgggcatcga
     7081 ggtgcgcttc gtcgatccgg ccgatcccga aggcttccgc cgcgccaccg acgagcgcac
     7141 gcggtgctat tacgcggaga ccttgcccaa ccccaagctc gaggtcttcc cgatcgccga
     7201 ggtggcccgg atcgggcgcg agctcggcgt gccgctgatc atggacaata ccgcggcgcc
     7261 ggtgttgtgc cggccgctgg atcatggcgc cgcgatcgtc atgcattcca cgacgaaata
     7321 catcggcggc catggcacgt cgatcggcgg catcgtcatc gacggcggca atttcgattg
     7381 ggaggcgaat gccaagcgct tcccgatgct caacacgccg gacccgagct atcacggggc
     7441 ggtgtggacg caggcggtca agccgctcgg acccatcgcc tatatcctca agatgcgcgt
     7501 caccctgctg cgcgacttgg gcgccgcgat gagcccgttc aacgccttcc tgttcctcca
     7561 gggcctcgag accctgccgc tccgcatccg cgcccattgc gccaacgcgt cacgcgtcgc
     7621 gaaatatctg agcgagcatt ccaaggtttc gcgcgtgata ttccccggcg ccatggaagg
     7681 ccgcgccaag aggcgcgcgg acacctatct gcgcggcggc tatggcggcc tcgtcggctt
     7741 cgagctcgcc ggcggaatgg aatcggggcg acgcttcatc gacgcgctca agatgttcta
     7801 tcacgtcgcc aatatcggcg acgcgcgcag cctcgccatt catccggcct cgaccacgca
     7861 ttcgcagctc tcgcccgaag agcagctcca gaccggcgtc agcgagcgct atgtgcggct
     7921 ctcgctcggc atcgagcatg aggaggacat cctcgccgac ctgtcgcagg cgctggacgc
     7981 ggcctgaggc gggcgggcgc cgcggcactt caccaagcgt tagtcgtggc gaatcgttac
     8041 gtgattgcga tggggggcaa gtttctcggc gcatccgctc tacccgaaag tgcaatggtc
     8101 cgcggcatcc caacggattg cggctggcct gagttctgtc gggcgcattc gccggctcgg
     8161 agaacgcctc cctttgggtg atacaagaga gccgggaaga tccctatgct cgtttgctta
     8221 agtcctccta cagaagcgtg aacggcgcgg gacggaacaa aaccgaaggg ggcggggctg
     8281 ccactttacg agagctatac gaccaccgtc gtcggggcgc atagcgttcc gcgctggtac
     8341 gaggcgctgg accggctggt gaccttcggg cagttggcgt ccggggattt cgaggacgct
     8401 cagttgcgct gttcgcaggc ggccatcttg gaacaggagc tcgcggggat cgacgtcatt
     8461 accggcggcg agatgcatcg gcggcggcac aaccggaatt ccccgcccaa cgccatgttg
     8521 aactatttct ggcagaagat tcccgcgttc cagggcgaga cgcgccccaa gcccatcact
     8581 aggcacgacc ccaacgtctt ccatcccgcc gccatttgcc gggggccgat cgccgaggat
     8641 atcgatctgg gactcgtcga agagttcaag accgtatcgg ctttcgcgca caagccggtc
     8701 aaggtcacta tgacgggccc gcatctcctc gccgcggtcg cctatgacga gtactacaac
     8761 gacctccccc ggatgatgtc cgacctcggc aagctgcttc accggaactt caagcttctc
     8821 gctgcggcgg gatgtaagca catccagatc gacgagccgt attttacggc ggcggaggat
     8881 gacgaggtgc gcgcggcggt cgaggtcatc aacatggcaa tcgaggggct tcccgaggat
     8941 gtccatgtgc tcgtgcatat ctgtcagggc aactacgcca tgggcgccga ctacgacggc
     9001 cagatcggtc atcgctattt cgacatgggc cgctacaaag ccgatctcat ctgccggatc
     9061 gagtgcgacg cctacctgat cgagcacgac atgacgcatc attatgaagg tttgctcggc
     9121 gacaggcagc ttggcgtggg cgcggtcgac gtgcagtcac ccaatgtcga gaccggggag
     9181 ctggtggctg atcgcatcgg ggcctatcgc tggctcgcgc ccgagcagac catcatcacc
     9241 agctcatgcg gcctcaacca cctgcccagg cacgtcgctc tcggcaagct tcgggccatg
     9301 accgaagcaa aagccatctt gcagcgtcgg cggtagagcc ggcggtccat ggctcaaaac
     9361 caagatgacg gcggcagtct cgaagcgcgc ctgcgggcga ccgaggagcg gctacggttg
     9421 gcagaggcag ccagcggtat cgcaatgttc gatctcgacc cagcgtctgg ggaaggggaa
     9481 cggacggctc aactggccgc gctcttcggc atcgatcagc aacaagagac gtcgtccttc
     9541 gccgaccggg taaaggccgt tttcgccgat gacgtgccga agctacgcgc ggccctcgag
     9601 attgccggcc gcaccggcac ctatcaggtg gaatttcggg tgaggcatgc cgacggcagc
     9661 gtccattggg tggccggaca tggcgccctg gtcaaggatg aggtcggccg cgcccgcctg
     9721 tgcggcgctt attacgagat caccgcccgg aagatgcttg aggcgcggtt actggcgctg
     9781 aacgagacgc tcgaagcgcg tgtcaccgag cgtgcgcggc agcttgcggc caacgccacc
     9841 caactcgaag agaccgagcg ccgctttcgg ctcctcgtcg acgcggtcac cgattacgcg
     9901 attttcatgc tcgatcccga gggcaacatc gtcagctgga atccgggcgc ccagcgcatc
     9961 aagggatacg acagggacga gatcgtcggc agccatttct cgcgcttcta cacggagcag
    10021 gatcgtgcga gcggcgtccc gggccgggcg ctcgcgacgg cgacacggac cggcaagttc
    10081 gaagccgaag gctggcgcgt ccgcaaggac ggcagcacct tctgggcgag cgtcgtcatc
    10141 aacgctatcc atggtcggga cggcagcctg ctcggctttg ccaaagtgac gcgcgacctg
    10201 acggagcggc gatccagcga ggagcggctg caccaggcgc agaagatgga agccgtgggt
    10261 cagctgaccg gcggcgtcgc ccatgatttc aacaacctgc tgacggtgat cacgggcaat
    10321 atcgactcgc tccagcgcca tcttccggag acggcggatc cgaaccttcg ccgcttcgcc
    10381 aattcggcgc tgcgtggggc cgagcgcgcg gcgctcctcg tgcacaggct cctggctttc
    10441 tcgcgccgcc agccgctggc gccgaaatcc gtatccgtca atacgctcat cgccggcatg
    10501 tcggagctgc tacgccgcac cctgggcgaa tccgtcgcca tcgagacggt gctcggcggc
    10561 ggcctctggc tggccttcat cgacgcgaac cagctcgaaa gcgcgctgct caacctcgcc
    10621 ctcaatgcac gcgacgcgat gcccgacggc ggtaaattga ccatcgaggc ggcgaatgtc
    10681 ttcctcgacg aggcctatgc ggctaacgcg gagatactac ctggtcagta tgtcggcctt
    10741 ttcgtcagcg acaccgggat cggcatgacg cccgaagtcg cagcgcacgc catcgacccg
    10801 ttcttcacga ccaaggagcc gggacagggg accggcctcg ggctctcgca ggtctatggt
    10861 ttcatcaaac agtccggcgg ccatctgaag atctacactg aggccggggc cggaacgacc
    10921 gtcaagctct atctgccgcg acacctcgca tcgcaggcgg agacgcaagc ggtgtccgcg
    10981 gcgcctgcca ttccgcgcgg gcggggcgaa gctattctcg tcgtggaaga cgatgcggac
    11041 gtccgcagct tcaccgtgga catgctccgg gagctggagt accgcgtcat cgaagcacat
    11101 gacggcgcct ccggtttgcg ccagctcgac gcccatcgcg atgtcgcgtt gctgttcacc
    11161 gatgtcggct tgccgggcgg catcaacggc cggcagctcg ccgacgaggc acggcggcgg
    11221 cggcccggcc tcaaggtgct tttcaccagc ggctatgcac gcaacgccat cgtgcatcac
    11281 ggccggctcg atccgggcgt cgagctgctg cagaaacctt tcgccttcgc tgcgctcgcc
    11341 gggcgcgtcc gcaccgcgct ggatgcacgc tgacgggaaa accggatcgc cggcgctcga
    11401 acgggctgat gcaggtcaag gcggctgctc ctcttccaga gtccattcgg gatcatggaa
    11461 ggcgaaatcg agaagctgca ccgcgacctg gaacgctcac caaggccctt cgctggctgc
    11521 tgggcggcgc caacggcaat agcaacggca acggcaaagg cgaaccgtac cttgtcgagc
    11581 cggctatttt ccggctcgtc ttcgcgatct tcgacggatt cagcgccaat atcggcgacg
    11641 ctcgcagcct cgccattcat ccggcctcga ccacgcattc gcagctctcg cccgaagagc
    11701 agctccagac cggcgtcagc gggcgctatg tgcggctctc gctcggcatc gagcatgagg
    11761 aggacatcct cgccgacctg tcgcaggccc tggacgcggc ctgacccttg cccactcctg
    11821 caatcaatcg cgcgcggcca gtcgccgctt gccggttccg tagtaggcga cgaaagcatc
    11881 gagcacctgc ttgaagaagc gtgggggcac gaagccgtag tcataacggt cgctgtgcgg
    11941 gagctttcgc aggtcgtagc cgggccagag aaactcgtta ccctcggcga cgacgatcca
    12001 tgaggcatcg tcgtccatcc ccagatgccg tttgacggcc gaaggtatct cgaccgccga
    12061 ggctggatcg gctggacggc cgtgggtgat cggcaggact gtgaccatgg tcgtgccgtc
    12121 cgcttcgcgc tcgaccgcca gcaggctgac gctcggcctg tctttgcgcc cctcttcgcg
    12181 cccggcctga tgttcgtggt gccagagata ggcgtaggag atgacgagac ccggttcagg
    12241 attaggaatc ggcatcccga tcttttattt tgattttgga tcgagcatcg tatcgagaga
    12301 ggcgtgccgc gcgtccatgc gggacgcgcc gatcgcttta actttctcct cggacagatc
    12361 cgcggtggcg aagctgcggc ggctttcgcg gaagcgtcgg aactcttcat attcatcggg
    12421 gccgacgaaa tagcccgtga tctggccgtg gctcgacacg gcgacagcct cgcgctgggc
    12481 gcgcatccga taacgcccaa aattgcgagt gaactcagaa gccgggacga tctttggtat
    12541 ggtcatcggg ctaatatacg cgatttgcgc aaattatgca aatagcttga attgcgttca
    12601 atgtcgaccg cgcattcgca gccctcgccc gaagagcagc tcaagactgg cgtcgagcgc
    12661 tatgtgcggc tctcgcttgg cgctggtggc ggcttgagag cgtgcttctc tcgaagcggg
    12721 aagagggcca cgcccgtcga gccggctctc cccccggctt cgtcttcgcg atcttcgacg
    12781 gcttcagaac ggcggtaatc gctcaaccgt agttggtgat ccgctcggat gccggcgcgc
    12841 ttccggcgtt ggcggcgtcg ccggtccgct tcgtcaccac cgtcgcgatc gcaaaggaga
    12901 ggttttcgag acggtagggc ttgtcgaggc gcgccatgtc ctggtcgtta ctgctcggca
    12961 cgtcggcata gccggtggcg agaatgatcg ggagtccggg ccaggtctcg cggatccggt
    13021 cggcaaggtc ggagccggtc atcccgggca tggcatggtc ggtgatgacg agatcgaccg
    13081 gcaccgccgc ctgcagcagt tcgagcgcgc cggcaccgga ggacgccacg accacccgat
    13141 ggccgagctg ctcgagcatc gccaccgtcg ctgtcgtgac ttcgacatcg tcgtcgacca
    13201 gcaggacgcg gcggggcggg accgggcttt gcacggctgc cgtcgctgcc tgcggcgcgt
    13261 gaatttcggt gctgtcggcg accggcagcc acagctcgac ggtcgtgccc actcccacgc
    13321 ggctcgcgat ccgcacggcg ccctccgatt gcgtcgccat gccatagacc atggagaggc
    13381 cgagaccggt gcctttgccc acgcccttgg tggtgaagaa cggctcggcc gcccgcttga
    13441 gcgtcgtctc gtccatgccg gtgcccccat ccgtgacgac gatgacgatg tattcgccgg
    13501 gaccagggcc ttggacgctg ccgctcacca cctcctcgcg gcgtgcgcca acggtgagac
    13561 ggccgccttg cggcatggca tcgcggccgt tgagggcgag gttcagcacc gcgagctcga
    13621 gctggttggg gtcgacgcgc accaaggcga ggtcttccgc gaaggccatg ccgatatcga
    13681 tggtgggccc gagcgagcgg cgcagcatct cgcccatgcc gttgatgagc cgcgccacat
    13741 cgaccgcttc gggcctgagc tcctggcggc gggcgaaggc gagcatgcgc ttcgtcagcg
    13801 tggcgccgcg ctcggccgcg cggatggccc cttcgatgag gcgcttcgcg ctggagtcgt
    13861 tcggcagcgt ccgcagcaga tcgagattgc cgaggacggc catgagcacg ttgttgaagt
    13921 catgcgccac gccgcccgtg agctggccca ggctttcgag cttctgcatc tcgtgcacct
    13981 gggcgatcgc cgcctcgcgc tcgcgcgtcc gctcctcgac gcgccgctcg aggtcggcat
    14041 tggtttgcgc caactcggcc gtccgctcgg cgacgcgtcg ctcgagctcg gcgttgagct
    14101 cgcggagctg cttggatttg cgatagagct ccgcgaagat ccgcaccttg gcgcgcagca
    14161 gctccggcac caccgggacc ggcacgtagt cgacggcgcc atgctcatag cccttgaggc
    14221 ggtcgagatc ggtcagcgct acggccgaga cgaagatgat cgccgtcgtc tcgaagcgcg
    14281 gatggttgcg caccatggtg gcaagctcga agccgtcgac ctccggcatg cggacatcga
    14341 ccagcatcac cgcgacattc ttcttcagaa gatgttcgag cgcctcctgg gccgatccgg
    14401 cgatcaggat gttctcgccg agatcgtcga ggatcgcctc gtagctcaag agcttggcgg
    14461 gctgatcgtc gaccaggaga atgttcaccg gctcttgcgt catgacccca ggttccttct
    14521 agcgatggag ccacatgcgc agcgccgaca gcagctgctc ggtgttcacg ggcttggcaa
    14581 gataatcgga cgcgccggct tcgaggcatt tctcgcggtc gcccttcatg gccttggccg
    14641 tcagggcgat gatgggcagc cgccggaacc cggacttggt gcggatcacc tgcatcgtct
    14701 catagccgtc catctccggc atcatgatgt ccatgagcac gatggcgacg ttcggcgtcg
    14761 attcgaggag agcgatcgcc tcgctgccgg tggtggcggt gagcacgtcc atgccgcgcc
    14821 gctcgagcac gctcgagagc gcgaagatgt tgcgggcatc gtcgtccacc agcagcaccg
    14881 tcttgccgac gaggtcctcg tccgagctgt ggagacgctc gagcagcgac tgcttctccg
    14941 ccggcagatc ggacacgacg cggtggagga agagggcggt ctcgtcgagg agccgctcgg
    15001 gtgattcgac gcccttgacc acgacgctcc gcgccatggt gtggagctgc gcgtcctctt
    15061 cgggcgagag ctcgcgtccg gtgaagacca cgaccggcac gtcggcgagc ccgacgtcgt
    15121 cgcggatctg ctccagcaat tggaaccccg acatgtcggg gagcctgaga tcgagaacga
    15181 cgcaatcgat cgagccttgg cgcaacgctt cgagcgcctc gctgccgctc tcgaccgaca
    15241 cgatctcgat gtcgtcatgc cccaggagct cggtgatgct gagccgctcg gcggcgttgt
    15301 cctcgaccac gaggagccga cggcggcgcc ggctcgtata ttccttgatg cgcgtcagcg
    15361 cctgctcgag cccttccttg ttggtgggct tggtgatgaa ggagaaggcg ccgcgcgcca
    15421 ggccgtgctg gcggtcctcg tcgagggtga ggatttgcac ggggatgtgg cgcgtcgtcg
    15481 ggttctgctt gagctggctc agcaccgtcc agcccagcat gtccggcagg aacacgtcga
    15541 gcgagacggc gctcggctga tactcgcgcg cgagcacgag cgcgtcgccg ccgcgcgccg
    15601 cgaccagcac cttgaagccc gaatcatggg cgaggtcggc catgatgcgc gcatagtgcg
    15661 gatcgtcctc gacgatcagc agcgtggttt cacccggctg gatgctggcg cggtcgtcgg
    15721 ggatggcctc cggcggcgct tcggcgagcc gcgcgcccgt gacggcggcg acagatgagg
    15781 cagcagtctg cgccacgcgt tgcggtgacg tgggccccgc atatttggtc ggcaggtaga
    15841 gcgtgaaagt gctgcccatg cccggggtcg atcgcagctg gatctcgccg ccgaggagat
    15901 tggcgagttc gcggctgatg gcgaggccga gcccggtgcc gccgtatttg cggctggtgc
    15961 tggcatcggc ctgctggaag gcctcgaaga tgatcttctg cttctc
//
LOCUS       DASYNS010000379         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2124018, whole genome shotgun sequence.
ACCESSION   DASYNS010000379 DASYNS010000000
VERSION     DASYNS010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2124018"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VGP50_11870"
     CDS             <1..811
                     /locus_tag="VGP50_11870"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEV8028122.1"
                     /translation="LGVAAEGIDLDRPLDGATTETLRRALLDNLVLCVRSQSLTPRSY
                     LAAASAFGHPITQLGVASRHPEVAEIMILSSEDRDAMGDGRPLVVGAHWHSDDSYRAM
                     PCSLTMLYGIEVPETGGDTQFANMYAAYEDLPPEMKEEIATLRVEHRYESSRRGTRIL
                     TLAADEAALVPEVTHPLVRVHPQTGHKALYLNPNRMERVVGLGRAESDRLLDALVAHA
                     VCERYQYRHKWRRGDVLIWDNRCTMHKANGDYALGARRFMHRIMIEGTVPV"
     gene            complement(849..1856)
                     /gene="dctP"
                     /locus_tag="VGP50_11875"
     CDS             complement(849..1856)
                     /gene="dctP"
                     /locus_tag="VGP50_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEV8028123.1"
                     /translation="MSPRRNRAAAGALLASTLALALCGAAAAATLQASHQFPGGKGDA
                     RDEMVQIIARELKAANVGLDVEVHPNASLYKPNDQWQAMVEGKLDISSFPLDYASGAE
                     HAFGATLMPGLVRNHDRALRLNDSRFMAEIREKIEKAGVIVLSDAWLGGAVASRKGCI
                     RTPADVKGLKIRSAGPTFAAMWQSGGASVVTLPSNEVYAALESGAIDATDTSAGSFVA
                     FHLFDQVKCLTAPGENALWFMYEPVLMSKKTFDGLSKQQQQALLAAGKKAEEYFAKAS
                     KGLDDEMVEIFKLHKVQVVTLTPTEYAAWISLARESSYAEFAKAVPDGKKLIDAALAV
                     Q"
BASE COUNT          315 a          632 c          633 g          315 t
ORIGIN      
        1 gctcggtgtc gcggccgagg ggatcgacct cgatcgcccg ctcgacggcg ccacgaccga
       61 gacactgaga cgcgcactct tggacaatct cgtcttgtgc gtgcgtagcc agtcgctgac
      121 gccgcggagc tacctcgctg ccgcgagcgc cttcggccat ccgatcaccc agctgggcgt
      181 cgcgtcgcgc cacccggagg ttgccgagat catgatcctc tcgagcgagg accgcgacgc
      241 catgggggat ggccgcccgc tggtggtggg cgcgcattgg cacagcgacg acagctatcg
      301 cgccatgccg tgctcgctca cgatgctcta tggcatcgag gtgccggaga ccggcggcga
      361 cacgcaattc gccaatatgt acgcggccta tgaggacctc ccgcccgaga tgaaggagga
      421 aatcgccacg ctgcgcgtcg agcatcgcta cgagtcgagc cggcgcggca cccgcatcct
      481 gacgcttgcc gccgatgaag cggcgctggt gccggaggtg acgcatccgc tggtgcgggt
      541 ccatccccag accggacaca aggcccttta tctgaatccg aaccgcatgg agcgggtcgt
      601 cggcctcggc cgcgccgaga gcgatcggct cctcgacgcg ctcgtcgccc atgcggtctg
      661 cgagcgctac caataccggc acaaatggcg gcggggcgac gtcctgatct gggacaaccg
      721 ctgcaccatg cacaaggcca atggcgacta tgctttggga gcgcggcgct tcatgcaccg
      781 catcatgatc gagggaacgg tcccggtgta agagtttgcc ctcgcccgcc ggcgcgagcg
      841 ggttggagct actgaacggc cagcgccgcg tcgatgagct tcttgccgtc gggcaccgcc
      901 ttggcgaact cggcgtagga gctctcccgg gcaagactga tccaggcggc atattccgtc
      961 ggcgtcagcg tcacgacctg gaccttgtgc agcttgaaga tctcgaccat ctcgtcatcg
     1021 aggcccttgc tggccttggc gaaatactcc tcggccttct tgccggccgc gaggagcgcc
     1081 tgttgctgct gcttgctcag gccatcgaag gtcttcttcg acatcagcac cggctcatac
     1141 atgaaccaga gcgcattctc gcccggcgcc gtcaggcatt tcacctggtc gaagagatgg
     1201 aaggcgacga agctgccggc gctggtgtcg gtggcgtcga tcgcgccgga ctcgagcgcg
     1261 gcgtacacct cgttgctggg caacgtcacc accgaggcgc caccgctctg ccacatcgcg
     1321 gcgaaggtgg ggcccgccga gcggatcttg aggcctttga cgtcggcggg cgtgcggatg
     1381 cagcccttgc gcgacgcgac ggcgccgccg agccaggcgt cagacagcac gatgacgccg
     1441 gccttctcga tcttttcgcg gatctccgcc atgaaacgcg agtcgttgag gcggagcgcg
     1501 cggtcgtgat tgcgcaccag gcccggcatg agcgtggcgc caaaagcgtg ctcggcgccg
     1561 ctcgcgtagt cgagtgggaa cgacgagatg tcgagcttgc cctcgaccat cgcctgccat
     1621 tgatcgttgg gcttgtagag cgaggcattc ggatgcacct cgacgtcgag cccgacattg
     1681 gcggccttca gctcgcgcgc gatgatctgc accatctcgt cgcgggcatc gcccttgccg
     1741 ccgggaaact ggtgcgacgc ctgaagcgtc gctgccgccg ccgcgccgca gagggcgagc
     1801 gcaagcgtcg aagcgagaag cgcgccggcc gcggcgcggt tgcgtctggg actcatgccg
     1861 aactctccca agatggaaca atcgaacggc gaagc
//
LOCUS       DASYNS010000380         3869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3060529, whole genome shotgun sequence.
ACCESSION   DASYNS010000380 DASYNS010000000
VERSION     DASYNS010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3060529"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1442
                     /gene="glcF"
                     /locus_tag="VGP50_11880"
     CDS             138..1442
                     /gene="glcF"
                     /locus_tag="VGP50_11880"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEV8028124.1"
                     /translation="MQTHFSLAQLADPDLREADKILRACVHCGFCTATCPTYVLLGDE
                     LDGPRGRIYLIKEMLEKGRAATEPVVKHIDRCLSCLSCMTTCPSGVNYQHLVDQARHH
                     IEQTYRRPLADRVLRGFLAAVLPRPRLFRLALRAAALARPIAWLLPARLRGIAALAPR
                     RVPAASPVDRPQVFPAQGTRKKRVALLAGCAQQVVAPQINEATIRLLTRHGCEVVIAR
                     GAGCCGGLTHHMGRPSLGFAKANIDAWTRVIEEGGLDALVVNASGCGVTIRDYGYLLR
                     EDAGYRDKAARISALTKDLSELLAEIGLAAPATVTGRRVAYHSACSLQHGLGVREAPK
                     ALLAKAGFVVLDVPEAHLCCGSAGTYNMLQPEIAERLRERKQRNIASTGAELVAAGNV
                     GCITQLAQGALPVLHTAELLDWATGGPMPTALSSGARPASGR"
     gene            complement(1408..2085)
                     /locus_tag="VGP50_11885"
     CDS             complement(1408..2085)
                     /locus_tag="VGP50_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEV8028125.1"
                     /translation="MTPAEDGKSPPLAALRMVGRLALPLLVIAGIGYAIANRHALDAD
                     SLAAAIGRYPAAPLVFLVVHTAASLVFFPRTVLGIAAGVLFGAWWGTLWAALGSTVGA
                     VAGFLLARYLNAGFVDLESMPRFGPILLRAERGGWRAVAVLRLIPVIPHSLSNYALGL
                     TRLNLGAYTLGSFVGQLPMTIACAQFGAAGEQLAAGKAGWVAPTLIGAVALAVTIIVP
                     KLASRRS"
     gene            complement(2082..2825)
                     /locus_tag="VGP50_11890"
     CDS             complement(2082..2825)
                     /locus_tag="VGP50_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV8028126.1"
                     /translation="MNLSVVIPVRNEAPNIAPLVAEIRAALDGRLDYEIIYVDDGSSD
                     DTAAVIRGLARDFPRLKLVRHARSCGQSTAVRTGVKAARAPWIATLDGDGQNDPADIP
                     RLWEIARAAPPTPPLLVAGYRQKRRDSAIKRVSSRLANAVRARLLGDGTPDTGCGLKL
                     FQRELFLELPFFDHIHRFLPALVQREGGRTVSVVVNHRPRARGTSNYGTFDRLWVGIT
                     DLLGVMWLKRRARNPEILAENDDELRRTG"
     gene            2929..3486
                     /locus_tag="VGP50_11895"
     CDS             2929..3486
                     /locus_tag="VGP50_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8028127.1"
                     /translation="MRALAFAAVLASAVVLAHGIASAGQDDPRLDKLFDTLKSTDSDE
                     EARRTEAEIWRVWIAHKNPEIEKLMRRGMAAFNEDDQEGALEAFNGVVQSDKSFAEGW
                     NKRATVEYAMGDFEASIADIERTLALEPRHFGALSGLGQIYLAMEKKALALKAFEAAL
                     KIDPHLSGVREKVEDLRKDLQGSPI"
     gene            3567..3860
                     /locus_tag="VGP50_11900"
     CDS             3567..3860
                     /locus_tag="VGP50_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEV8028128.1"
                     /translation="MSLSHGGVLSERIIKMAIDVHRHLGPGLLESAYEGCLCYELDQG
                     GVAYARQVPLAIVYKEVRLDCGYRMDIVAERELLIEVKAVERLMPIHEAQILT"
BASE COUNT          658 a         1264 c         1339 g          608 t
ORIGIN      
        1 cccaagcgca tcttgaaccg cggccgcatg tatccggagc tgtgatgctg ccaatgtcgc
       61 cactgcgcca aatcctgcgc cgccccctca ccccgaccct ctcccgcaag cgggagaggg
      121 ggaagatata gctacccatg cagacgcatt tctcgctggc gcaactggcc gatcccgatc
      181 ttcgcgaggc cgacaagatt ctccgcgctt gcgtccattg cggcttctgc acggcgacct
      241 gcccgaccta tgtgctgctg ggtgacgaac tcgacgggcc gcgcgggcgc atctatctca
      301 tcaaggagat gctggagaag gggcgggcgg cgacggagcc cgtggtgaag catatcgatc
      361 gctgcctgtc ctgcctgtcc tgcatgacga cctgcccctc gggggtgaat taccagcatc
      421 tggtcgacca ggcgcgccat cacatcgaac agacctatcg gcgtcctctg gcggaccgcg
      481 tcttgcgcgg cttcctcgcg gccgtgttgc cgcggccgcg cctcttccgc ctggccttgc
      541 gcgccgccgc gctggcgcgg ccgatcgcat ggctgctgcc ggcgcgcttg cgcgggatcg
      601 cagcgctcgc gccgcggcgg gttccggccg cctcaccggt cgatcggccg caggtcttcc
      661 cggcgcaagg gacacgcaag aagcgcgtgg cgcttctcgc ggggtgcgcg cagcaggtgg
      721 tggcgccgca gatcaatgaa gcgaccatcc gcctcctgac gcgtcacggc tgcgaggtgg
      781 tgatcgccag gggagcgggg tgctgcggcg ggctcacgca tcatatgggc cgtccctcgc
      841 tcggcttcgc caaagccaat atcgatgcct ggacgcgggt gatcgaggag gggggcctcg
      901 acgcgctggt cgtcaatgcc tcgggctgcg gcgtcaccat ccgggactat ggctacctgc
      961 tgcgcgaaga cgcggggtac cgcgacaagg cggcgcgcat ctcggcgctg accaaggatt
     1021 tgtccgagct tcttgccgag atcggactcg ccgctccggc gaccgtgacc gggcggcgcg
     1081 tcgcttatca ttcggcctgc tcgctgcagc atggcctggg cgtccgcgag gcgcccaagg
     1141 cgctgctcgc caaggcgggc ttcgtcgtgc tcgacgtgcc cgaagcgcat ctctgctgcg
     1201 gctcggccgg cacctacaac atgctgcagc cggagatcgc cgagcgcctg cgcgagcgga
     1261 aacagcgcaa tatcgcgagc accggcgccg agctggtcgc cgcgggcaat gtcggctgca
     1321 tcacgcagct cgcgcagggc gcgctgcccg tgctgcatac ggccgaactc ttggactggg
     1381 cgacgggcgg accgatgccg accgcgctca gctccggcgc gaggccagct tcgggacgat
     1441 gatcgtgacg gcgagcgcaa cggcgccgat cagcgtcggc gcgacccatc ctgccttgcc
     1501 ggcggcgagc tgctcgccgg cggcgccgaa ctgggcgcag gcgatggtca tgggcagctg
     1561 gccgacgaag gaaccgagcg tgtaggcgcc gagattgagg cgtgtcaggc ccagcgcata
     1621 attcgagagc gaatgcggga tgaccgggat gagacgcagc accgctaccg cccgccagcc
     1681 gccgcgctcg gcgcgcagca ggatcgggcc gaagcggggc atgctctcga gatcgacgaa
     1741 gccggcattg aggtagcgcg ccaggagaaa gccggcgacg gcgcccacgg tgctgcccag
     1801 cgctgcccat agggtgcccc accaggcccc gaacagcacg cccgccgcga tgccgagcac
     1861 ggtgcgcgga aagaagacga ggctcgccgc ggtatggacg acgaggaaga ccagcggcgc
     1921 tgccggatat cggccgatgg ccgccgccaa ggaatcggcg tcgagggcat ggcgattggc
     1981 gatcgcgtat ccgatgccgg cgatcaccag cagcgggagc gccagccgtc ccaccatgcg
     2041 cagcgccgcc agcggcggcg atttgccgtc ctcggccggg gtcatccggt gcgcctcagc
     2101 tcatcgtcgt tctcggcgag gatctccggg ttcctggcgc gtcgcttcag ccacatcacg
     2161 cccaggagat cggtgatccc gacccaaagg cggtcgaagg tgccgtaatt ggatgttccg
     2221 cgcgcccggg ggcgatggtt cacgacgacg gagacggtgc gcccgccctc gcgctgcacc
     2281 agcgccggca ggaagcgatg gatgtgatcg aagaaaggca actcgagaaa gagctcgcgc
     2341 tggaagagct tgagcccgca gccagtgtcg ggcgtgccgt cgcccaggag ccgcgctcgc
     2401 accgcgttgg cgagccggga cgacacgcgt ttgatcgcgc tgtcgcgccg cttctggcgg
     2461 tacccggcga cgagcagcgg cggtgtcggc ggcgccgcgc gggcgatctc ccaaagccgc
     2521 gggatatcgg cgggatcgtt ctggccgtcg ccatcgaggg tggcgatcca cggcgcgcgc
     2581 gccgccttga cgcccgtcct gaccgccgtg ctctggccgc agcttcgcgc atggcggacc
     2641 agcttgaggc gggggaaatc gcgggccagg ccgcggatca cggcagcggt gtcatcgctg
     2701 ctgccgtcat cgacgtagat gatctcgtag tcgagccggc cgtcgagcgc cgcacggatc
     2761 tcggcgacca ggggcgcgat gttcggcgct tcgttgcgga cggggatgac gaccgacagg
     2821 ttcatggcgc gaggcatcta gcacagttct cgaggacggc aaaattcgca ggcgggcggc
     2881 ccaacccgcc cattggcggc aagaatgctc cagtctatca tttgttccat gcgcgctctc
     2941 gcctttgccg cggtcctggc ctcggccgtc gtcctcgccc acggcatcgc ttccgccgga
     3001 caggacgatc cgcggctcga caagctcttc gacacgctca aatccacgga ctccgatgaa
     3061 gaagcgcgac gcaccgaggc ggagatctgg cgcgtctgga tcgcgcacaa gaatcccgag
     3121 atcgagaagc tgatgcgccg cggcatggcg gccttcaacg aagacgacca ggagggcgcg
     3181 ctcgaggcgt tcaatggcgt cgtgcaatcg gacaagagtt tcgccgaagg ctggaacaag
     3241 cgcgccaccg tcgaatatgc gatgggcgat ttcgaggcca gcatcgccga tatcgaacgc
     3301 acgctggcgc tcgagcccag gcatttcggc gcactgtcgg gtcttggcca gatctatctc
     3361 gcgatggaga agaaggcgct ggcgctcaag gctttcgaag cggcgctgaa gatcgacccg
     3421 catttgagcg gtgtccgcga gaaggtcgag gacctgcgca aggatctcca gggcagcccg
     3481 atctagcacg gcctttcccg gcgacggcga atagggtgga cggcgggtgt caccacggag
     3541 acacggagga cacggagaag aataacgtga gtctttctca tggcggcgtc ctgagcgagc
     3601 gcatcatcaa gatggcgatc gatgttcatc ggcatctggg acctggattg ctcgaatcgg
     3661 cttacgaggg atgtctgtgc tacgagctgg accagggtgg agttgcctat gcgcgccaag
     3721 tgccgcttgc aattgtctac aaggaagtcc ggctagattg cggctatcgg atggatatcg
     3781 tcgccgaacg agagctcctc atcgaagtca aggcagtcga acgactcatg cctattcacg
     3841 aagcccagat actgacttaa tctgcgcct
//
LOCUS       DASYNS010000381        10027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1659175, whole genome shotgun sequence.
ACCESSION   DASYNS010000381 DASYNS010000000
VERSION     DASYNS010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659175"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..3671)
                     /gene="mfd"
                     /locus_tag="VGP50_11905"
     CDS             complement(276..3671)
                     /gene="mfd"
                     /locus_tag="VGP50_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV8028129.1"
                     /translation="MARIAAALAFFHPSLEVVALPAWDCLPYDRVSPNAEAMSRRIDA
                     LTRLAEPALPAQRRIVLTTVSAALQRVPPRKLFQDRVLRLGRGGRIPLDRLISFLDKN
                     GYGRSDTVREAGEFAVRGGIFDLFPSGAPAPVRLDFFGDTLEAIRSFDALSQRSTGAF
                     DTVVLRPVNEVLLDDAAIQRFRTRYREQFGTVADDDPLYAAISAGRHFIGMEHWLPLY
                     YETLETLFDYLPDAAVTLDHQADDVCRDRIAHIADSYLARVEAMRARSTAAPIYRPVR
                     PDQLYLEDKEWSRALAQRRTAAFTPFTVPADAPLVVDAGARPGHGFAEARANPNLNLF
                     EVVREHIGDLAKAGRRTLIVAYSAGSAERLGSLLRERGLESLETVASWEAFERLGGES
                     AGIAVLALEHGYVFDGRAILTEEDMLGDRLTRPPPRRRANFDQFIAEVSSLQAGDLMV
                     HVEHGIGRYDGLVTLQVAGAPHDCLRVIYAGDDKLFVPVENIEVLSRYGSEEADVQLD
                     RLGGVAWQSRKARVKERIKSIAGELIAVAAQRQLRQGEPMPPPEGLYEEFAARFPYPE
                     TDDQLKAILDTLSDMASGKPMDRLICGDVGFGKTEVALRAAFIAVMAGSQVAVVVPTT
                     LLARQHYRTFSERFAGLAVHVAQLSRLVSARDAAAIKKELAEGRLDIVVGTHALLGKG
                     LKFPHLGLLVVDEEQHFGVTQKERLKQLKANVHVLTLTATPIPRTLQLALAGVREMSV
                     IATPPVDRLAVRSFVMPYDPVVLREAILREHHRGGQIFYVAPRIGDLDDLSLALKELV
                     PEIKVAVAHGRMPAAELERVMGAFDDGAFDLLLSTNIIESGLDIPSANTLIVHRADMF
                     GLGQLYQLRGRVGRSKQRAYAYLTLPTGKTLTATAQRRLEVMQTLDQLGAGFTLASHD
                     LDIRGAGNLLGEEQSGHVREVGVELYQHMLEEAVAAARSGVDQLGEAVEEWTPQITIG
                     TPVLIPESYVADLNVRLGLYRRIAALLDRREIDAFAAELVDRFGPLPAEVDNLLEIIA
                     IKRLCRDAGVEKVEAGPKGAVLAFHDNHFANPAGLVAFLQEQVGTAKLRPDQRLVIMR
                     NWEETRDRLRGINALLRRLSEIAVAPRAALPPAVAAPKRGSAR"
     gene            complement(3830..4099)
                     /locus_tag="VGP50_11910"
     CDS             complement(3830..4099)
                     /locus_tag="VGP50_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEV8028130.1"
                     /translation="MEDALEVRRKRLLFRSWRRGTRESDLLLGSFAGQHVAAWGAAEL
                     DRYEALLEASDAEIFDWIAGRVAPPAERDTDILRLLVNFRYHRSA"
     gene            complement(4176..4556)
                     /locus_tag="VGP50_11915"
     CDS             complement(4176..4556)
                     /locus_tag="VGP50_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEV8028131.1"
                     /translation="MAERRGEDPLTEKLIGFAIEVHRHLGPGLLESAYEECLCYELEH
                     SGVPFKRQVPLPVVYKSVRLDCGYRLDIIIDEKVIVELKTVERLLPVHDAQVLTYLKL
                     SGIRTGLLLNFYSPMLKDGIRRFI"
     gene            4633..6714
                     /gene="recG"
                     /locus_tag="VGP50_11920"
     CDS             4633..6714
                     /gene="recG"
                     /locus_tag="VGP50_11920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV8028132.1"
                     /translation="MRPEVLNPLFASVTSLPGVGPRFGKLIEHLAGPCAVDLLWHLPN
                     GIIDRSFTPKVREAPAGAIATLKVIVDAHLRPHSPRAPYRVRCRDETGFLHLVYFHVK
                     GDYLEKLLPVGSVRIVSGRVEHFNNEIQITHPDHIVDPEAAESLKPIEPVYGLTAGLP
                     QRLVQKAVAAALARLPRLPEWLDRDLVRQRGWQAWADALAAAHAPESEHDLAPESPPR
                     QRLAYDELLAGQLAIALVRAHTRRLAGRAIKAEGKLREKVMAALPFALTNSQRQALAE
                     IGGDMASPLRMLRLLQGDVGSGKTVVALLAMLDAVEAGGQAALMAPTEILARQHFATL
                     SKLGAPAGVTFALLTGREKGGARKSLLERLANGEIGIVVGTHALFQEDVAFKDLTLAV
                     IDEQHRFGVEQRIALSGKGRGVDILVMTATPIPRTLLLTAYGDLDTSRLTEKPAGRKP
                     VATRTLPATRLGEVIEAVRRALAQGAKIYWICPLVEESENVDLAAAEDRAVELKSLFG
                     ERVGLVHGRMKGTDKDRTMEAFASGDVDLLVATTVIEVGVDVADATVMVIEHAERFGL
                     AQLHQLRGRIGRGDRPSACLLVYAVPLGETAKARLTLLRETEDGFRIAEEDLRLRGGG
                     EVLGTRQSGLPELRLADLVAHAELLAVARDDARLVLSRDPELETPRGQALRLLLYLFQ
                     RELAVRYVRSG"
     gene            6853..8121
                     /locus_tag="VGP50_11925"
     CDS             6853..8121
                     /locus_tag="VGP50_11925"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028133.1"
                     /translation="MIQSARLSGPDYRLVFDNAPWPCLVLQPDLVIVAASNAYLDATE
                     MQREAILGRPLLEAFASVADDPSGETLLALRTSLERVLHTRRPDGIAAASQGVRPRSW
                     SLVSSPVLDESGVIAYIVHRIDDTTDMVQLKRAGREHSRMTRRLALRLQAMEHDIDER
                     AQLLDAANERLRATRLGPHEDVMRQLAGAVAHDFNDLMTVIAGSLSVIEDFPGAPSDL
                     LQLAAGMQRAIERGSRLTNQLLAVGRGLAALPDPLALNELLRDFSATAAPPPAMGERS
                     ARRRAAASEPVGAPSAARSSANRLVGRCGRILVVEDDPDVLTSVLGAMTRLGYGTVAA
                     RDGHEALAALGGSEPLDLLFTDIVMPKGMNGIQLARQARELRPNLPVLLTSGYGFHAL
                     SAGADDGFPLLQKPYGRQSLAARLRDLIGS"
     gene            complement(8118..9020)
                     /locus_tag="VGP50_11930"
     CDS             complement(8118..9020)
                     /locus_tag="VGP50_11930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEV8028134.1"
                     /translation="MTLEPKLADPVAVAIPAFVLLVLLEIAVAWRRRLPAYELHDTAA
                     SLAMGLGNLAIGLAGGAVAYVALNWVYQFRLITLGYAWESFVLILFAEDLTYYWFHRV
                     SHRCRFWWAAHINHHSSQHYNLSTALRQSWTGGLFEWVLWLPLTLVGFPPALVVFQRG
                     VSLVYQFWIHTETVGRLPLWIEAVMNTPSHHRVHHATNPRYLDRNYAGVFILWDRLFG
                     TFVAEAPEDPCRYGIVKNIGSFNPLRIALHEWVAIAKDVAAAQSWRERLVYLLGQPGR
                     SFDGSRHTSDAIRAAWQARLARTG"
     gene            complement(9017..9427)
                     /locus_tag="VGP50_11935"
     CDS             complement(9017..9427)
                     /locus_tag="VGP50_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028135.1"
                     /translation="MDPTNPLTLLVPLIGIALILTAVWLTGARPARLDRALVMARLGE
                     DLPDFVADEIVIDAGRTTALAAGADGTLVVVFAAGDRVVVRALERGEVRRVGVDGDRL
                     TVDTDAFTHRCFAMMLPGAASRWARRLLPSAGRA"
     gene            9573..9755
                     /locus_tag="VGP50_11940"
     CDS             9573..9755
                     /locus_tag="VGP50_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028136.1"
                     /translation="MARKSTLCRVTMLATILLLAGCASPHGTYSNPYGPRNGDAMLVD
                     PHTGVVLPGQSDPGGD"
BASE COUNT         1726 a         3354 c         3373 g         1574 t
ORIGIN      
        1 ccggcgccgc ccgcgccggc ggcataggcc ttcttcgaca gcgtcagcgt gtcaccggtt
       61 ttcgagatcg ggagcgccgc gaacagcaac gcgagtacgg cggtcgagac gagcaacgca
      121 gtcttgtggc tctgatgcgc gcgcatgatt tgccccgtaa cgcagagaat ctgcgcaccg
      181 agaaccctcg catttggatt ccgttccatc ggcgcgagaa gcacagctgc cggtttcaca
      241 tcggcgtgcc cgcatcaacc ggcgatgcag cgcggtcagc gcgcgctgcc gcgtttgggc
      301 gctgcgacgg cgggcgggag cgcggcgcgc ggcgcgaccg cgatctccga gagtcggcgc
      361 agcagcgcgt tgattccgcg cagccggtcg cgcgtctctt cccagttgcg catgatgacg
      421 agccgctggt cgggcctgag cttggcggtg cccacctgct cctgtaggaa ggcgacgagc
      481 cccgccggat tggcgaaatg attgtcgtgg aaggcgagaa cggcgccctt gggaccggcc
      541 tccactttct cgacgccggc gtcgcggcag aggcgcttga tggcgatgat ctcgaggagg
      601 ttgtcgacct ccgccggcaa aggaccgaag cggtcgacca gctcggcggc gaaggcgtcg
      661 atctcgcggc ggtcgagcag cgcggcgatg cggcgataga gtccgagccg cacgttgaga
      721 tcggcgacat agctctcggg aatgagcacc ggcgtgccga tggtaatctg cggcgtccat
      781 tcctcgacgg cttcgccgag ctggtcgacg ccgctccgcg cggcggcgac cgcctcttcc
      841 agcatgtgct ggtagagctc gacaccgacc tcgcggacat ggcccgactg ctcctcgccg
      901 agcagattgc cggcgccgcg gatgtcgaga tcgtggctcg ccagcgtgaa gccggcgccg
      961 agctgatcga gcgtctgcat cacttcgagc cggcgttgcg ccgtcgccgt cagcgtcttg
     1021 ccggtgggca gcgtcagata ggcataggcg cgctgcttgc tgcgtccgac ccggccgcgc
     1081 agctgataga gctggcccaa accgaacatg tcggcgcgat ggacgatgag cgtgttggcc
     1141 gagggaatgt cgaggccgga ctcgatgatg ttggtggaca gcagcaggtc gaaggcgccg
     1201 tcgtcgaagg cgcccatgac gcgctcgagc tcggccgccg gcatccggcc atgggccacc
     1261 gcaaccttga tctccggcac gagctccttg agcgcgaggc tcagatcgtc gagatcgccg
     1321 atgcgcggcg ccacatagaa gatctggccg ccgcggtgat gctcgcgcag aatcgcctcg
     1381 cgcagcacca cgggatcgta aggcatgacg aagctgcgca ccgcgaggcg atcgacaggc
     1441 ggcgtggcga tgacgctcat ctcgcgaaca ccggccaagg ccagctgcag cgtgcgcgga
     1501 atcggcgtcg cggtcagcgt cagcacatgg acattggcct tgagctgctt caggcgctcc
     1561 ttctgcgtca cgccgaaatg ctgctcctcg tcgaccacga gcaggccgag atgcggaaat
     1621 ttgaggcctt tgccgaggag cgcgtgagtc ccgacgacga tgtcgaggcg gccctccgcc
     1681 agctccttct tgatcgccgc cgcgtcgcgc gccgagacca gacgagagag ctgggcgacg
     1741 tgcaccgcaa gcccggcgaa gcgctcgctg aaggtgcggt aatgctggcg cgccagcagc
     1801 gtcgtcggca cgacgacggc gacctgggag cctgccatca cagcgatgaa ggccgcgcgc
     1861 agcgccacct ccgtcttgcc gaagccgaca tcgccgcaga tcaggcgatc catcggcttt
     1921 cccgacgcca tgtcggagag cgtgtcgagg atggccttca gctgatcgtc ggtctcggga
     1981 taagggaagc gcgcagcgaa ctcctcatag aggccttcgg gcggcggcat cggctcgccc
     2041 tggcgaagct ggcgctgcgc cgcgacggcg atgagctcgc cggcgatgct cttgatgcgt
     2101 tccttgacgc gcgccttgcg cgactgccag gcgacgccgc ccaggcgatc gagctgcaca
     2161 tcggcctctt ccgagccata gcgcgacagc acctcgatat tctccacggg cacgaagagc
     2221 ttgtcgtcgc cggcatagat gacgcggagg cagtcatggg gcgcccctgc cacctggagc
     2281 gtgacgagcc cgtcatagcg gccgatgccg tgctccacat gcaccatgag atcgccggcc
     2341 tggagcgacg agacctcggc gatgaactgg tcgaaattgg cgcggcgccg cggcggcggg
     2401 cgggtgagcc ggtcgcccag catgtcctct tcggtgagga tggcgcgacc gtcgaagaca
     2461 tagccatgct cgagcgccag cacggcgatg cccgcgctct cgccacccag ccgttcgaac
     2521 gcctcccagc tcgccaccgt ctcgagcgac tcgaggccgc gctcgcgcag cagcgacccg
     2581 aggcgctcgg cggagccggc gctgtaagcg acgatcagtg tgcgccggcc ggccttggcg
     2641 agatcgccga tatgctcccg gaccacctcg aagaggttga gattgggatt ggcccgcgcc
     2701 tcggcgaagc cgtggcccgg ccgcgctccg gcatcgacga cgaggggtgc gtccgccggc
     2761 accgtgaacg gcgtgaacgc tgccgtgcgg cgttgggcga gcgcccgact ccactcctta
     2821 tcctcgagat agagctgatc cggccgcacc gggcgataga tcggggctgc cgtgctcctc
     2881 gcccgcatgg cctcgacacg cgccaggtag gagtcggcga tatgcgcgat gcggtcgcgg
     2941 cacacgtcat ccgcctgatg gtcgagcgtc acggcggcat cgggcagata gtcgaacagg
     3001 gtctcgagcg tctcgtaata gagcggcagc caatgctcca tgccgatgaa gtgacggccg
     3061 gcgctgatcg cggcatagag cggatcgtcg tcggcgaccg tgccgaactg ctcgcgatag
     3121 cgtgtgcgga agcgctggat ggccgcatcg tcgagcagca cctcgttgac ggggcgcagg
     3181 acgaccgtgt cgaacgcgcc ggtgctgcgc tggctcagcg catcgaaact gcggatcgcc
     3241 tcgagcgtgt cgccgaagaa gtcgaggcgg accggcgccg gcgcgcccga aggaaaaaga
     3301 tcgaagatgc cgccgcgcac cgcgaactcg ccggcctcgc gcaccgtgtc gctgcggcca
     3361 taaccgttct tatcgagaaa gctgatgagg cgatcgagcg ggatgcgccc gccgcggccc
     3421 aagcgcagga cgcggtcctg gaagagcttc cgcggcggca cgcgctgcag cgccgcgctc
     3481 accgtggtca gcacgatgcg ccgctgcgcc ggcaatgccg gctccgcgag ccgcgtcaag
     3541 gcgtcgatgc gtcggctcat cgcctcggcg ttgggcgaga cgcggtcgta ggggaggcag
     3601 tcccaggccg gcagcgccac gacctcgagc gacggatgga agaaggcgag cgccgcggcg
     3661 atgcgcgcca tgcgtccttc gtcgcggctc acatggagca ggctcggcac gtgtcctgcc
     3721 gcgacgagcc cgcccaggac gaacgcatca tgcccttcgg gcgcccctga gatggtgagg
     3781 cgtccggcgg gaagcggccg gtcgggcgcg aagaattgca agagggtggt cacgcggagc
     3841 ggtgatagcg gaaattcacg agaaggcgaa ggatgtcggt gtcgcgctcg gcgggcgggg
     3901 caacccggcc ggcgatccag tcgaagatct ccgcatcgct cgcctcgagc agcgcctcat
     3961 agcgatcgag ctccgccgcg ccccatgcgg cgacgtgctg cccggcgaag ctgcccagca
     4021 gcagatcgct ctcgcgcgtg ccgcgccgcc agctgcggaa aaggagccgc ttccggcgga
     4081 cctccaatgc atcctccatg ctgtactccg atatagcgag tcttcgcaag attagaagat
     4141 tgaccacaga ggcgcagaga cacagaggaa gagttctaaa tgaagcgccg aatcccgtcc
     4201 ttgagcatcg gagaatagaa gttgaggagc aatcccgtgc gaatgccgct gagtttcaaa
     4261 taggttagca cttgcgcgtc atggaccggt agcaggcgct ccacggtctt cagttcgacg
     4321 atcacctttt catcaatgat gatatcaagg cgataaccgc aatccagccg aaccgattta
     4381 tagacgacgg ggagaggcac ctgccgtttg aaaggaaccc cgctatgctc aagctcatag
     4441 cacaagcatt cctcataagc agattccaac agaccgggcc cgagatgccg atgcacctct
     4501 atcgcaaagc cgatgagctt ctccgtgagc gggtcttctc cgcgcctctc cgccatggtg
     4561 ctcggtcctc tgtgcctctg tgcctctgtg gtcaatctta gtctttcccg atgtgtcctt
     4621 taacctttcc cgatgcgtcc tgaggttctc aatccgctgt ttgcgtcggt gacgtcgctg
     4681 ccgggcgtcg gaccgcgatt cggcaagctc atcgagcatc tcgccggacc ctgcgcggtc
     4741 gatctgctgt ggcatctgcc gaacggcatc atcgatcgca gtttcacgcc caaagtgcgg
     4801 gaggcgccgg ccggcgccat cgccacgctc aaagtcatcg tcgacgcgca tttgaggccg
     4861 cacagcccgc gcgcgcccta tcgcgtgcgc tgccgcgacg agaccggctt cctccacctc
     4921 gtctatttcc acgtcaaggg cgactatctc gagaagctgc tccccgtggg gtcggtgcgc
     4981 atcgtcagcg gccgcgtcga gcatttcaac aatgagatcc agatcaccca tcccgaccac
     5041 atcgtcgatc ccgaagcggc cgagagcctg aagccgatcg agcccgtcta tggcctcacc
     5101 gccggcctgc cgcagcgcct cgtgcagaaa gccgtcgccg cggcgctcgc gcgcctgccg
     5161 agacttccgg agtggctcga ccgggacctg gtgcgccagc gcggctggca ggcttgggcc
     5221 gacgcgctgg ccgccgctca tgcccccgaa agcgagcacg acctcgcgcc cgagtctccg
     5281 ccgcgccagc gcctcgccta tgacgaattg ctggcgggac aactcgcgat cgcgctggtg
     5341 cgtgcccata cgcggcgcct cgccggccgg gccatcaagg ccgagggcaa gctccgggag
     5401 aaggtgatgg ctgcgctccc cttcgccctc acgaactcgc aacgccaggc gctcgccgag
     5461 atcggtggcg acatggcctc gcccttgcgc atgctgcgcc tcctccaagg cgatgtcggg
     5521 agcggcaaga ccgtggtggc gctgctcgcc atgctcgacg ccgtcgaggc gggcggccaa
     5581 gccgcgctca tggcgccgac cgagatcctg gcgcgccagc atttcgcgac gctgagcaag
     5641 ctcggcgcgc ccgcaggcgt gactttcgcg ctgctcacgg gacgcgagaa aggcggcgcg
     5701 cgcaagagct tgctcgagcg gcttgccaat ggcgagatcg gcatcgtcgt cggcacccac
     5761 gcgctctttc aggaagacgt cgccttcaag gacctcacgc tcgcggtcat cgacgagcag
     5821 caccgcttcg gcgtcgagca gcgcatcgcg ctgtccggca aagggcgcgg cgtcgacatc
     5881 ctcgtgatga cggcgacgcc catcccgcgc acgctcctcc tcacggcgta tggcgatctc
     5941 gatacgtcgc ggctgaccga gaaaccggcc ggccgcaagc cggtggcgac gcgcacgctg
     6001 ccggcgacgc ggctcggcga ggtgatcgag gcggtccgtc gcgccttggc gcagggcgcc
     6061 aagatctatt ggatctgccc gctggtcgag gaatccgaaa acgttgacct cgccgcggcc
     6121 gaggaccggg ccgtcgagct caagagcctt ttcggcgaac gcgtcggcct cgtccatggc
     6181 cgcatgaaag gcaccgacaa ggaccggacg atggaagcct tcgcctcggg cgacgtcgac
     6241 ctgctggtcg cgacgaccgt gatcgaggtc ggcgtcgacg tggcggatgc aaccgtcatg
     6301 gtgatcgagc acgccgagcg tttcggtctc gcgcagttgc atcagctgcg cggccggatc
     6361 ggccggggcg atcggccttc ggcctgcctt ctggtctatg cagtgccgct cggcgagacg
     6421 gcgaaggcgc gactgacgct gttgcgcgag accgaggacg gcttccgcat cgcggaagag
     6481 gatctccggc tccgcggtgg tggcgaggtg ctgggcacgc gccagagcgg ccttccggag
     6541 ctgcggctcg ccgatctcgt tgctcatgcc gagctgctgg ccgtggcgcg cgacgatgcg
     6601 cgcctcgtcc tctcacgaga tcctgaactc gaaaccccgc gcggtcaggc actgcggttg
     6661 ttactctact tgttccaacg cgagcttgcc gtgcgctacg taagatctgg atgactcccg
     6721 gttgtagacg cgatctagcc agagtggtat gttcgtccat aggtatttag gtcgtggccc
     6781 ctagcgtacc atttgttgtg gagccaatga ttgggagccc aacagaagcc aggaaactca
     6841 gggagggaac acatgatcca atctgcgcgg ttgagcggcc cggattaccg gctggtcttc
     6901 gacaacgctc cttggccctg cctcgttctt cagccggacc tggtcatcgt cgccgccagc
     6961 aatgcctatc tcgacgccac cgagatgcag cgggaagcga tcctcggtcg gccattgctc
     7021 gaggccttcg cgagcgttgc cgacgatccc tcgggcgaga ctttgctcgc ccttcgcacc
     7081 tcgctcgagc gcgtgctgca cacgcgccga ccggacggca tcgctgccgc gagtcagggc
     7141 gtacgccccc ggagctggag tctcgtcagt tcgcccgtgc tcgacgagtc cggcgtcatc
     7201 gcatatatcg tccaccgcat cgatgatacg accgacatgg tccagctgaa gcgcgccgga
     7261 cgcgagcatt cgcgcatgac acggcgcctc gccttgcgcc ttcaggcgat ggagcacgac
     7321 atcgacgagc gcgcgcagct gctggacgca gccaatgagc gattgcgcgc caccaggctc
     7381 ggaccccacg aggacgtcat gcgccagctc gccggcgccg tcgctcatga cttcaacgac
     7441 ctcatgacgg tcatcgccgg cagcttgagc gtgatcgagg atttccccgg cgcgcccagc
     7501 gacctgctgc agctcgccgc cgggatgcag cgtgcgatcg agcgcggctc gcggctcacc
     7561 aaccagctcc tcgctgtggg ccgcgggctt gcggcgctgc ccgaccccct cgccttgaat
     7621 gagctgctgc gcgacttctc cgcgaccgct gcgccgccgc ccgccatggg cgagcgctcg
     7681 gcaaggcggc gcgccgccgc ttccgagccg gtcggagcac catcggcagc gcgcagcagc
     7741 gcgaaccggt tggttggccg ctgtggacgg atcctcgtcg tcgaggacga cccggacgtg
     7801 ctcacgtcgg tgctcggcgc catgacgcgg ctggggtacg ggaccgtggc ggcacgcgat
     7861 ggccatgagg cgctggctgc gctcggcggg tccgagccgc tcgatctgct gttcaccgat
     7921 atcgtcatgc cgaagggaat gaacggcatc cagctggcgc gccaggcccg cgagctcagg
     7981 ccgaacctcc cggtgctttt gacctcgggc tatggatttc atgcgctcag cgcgggcgcg
     8041 gacgacggct tccccctcct gcagaagccc tatgggcgcc aatcgctggc ggcgaggctg
     8101 cgcgacctga tcgggtccta gccggtccgg gcgagacgcg cctgccaagc ggcgcgaatc
     8161 gcgtcgctgg tgtggcgcga gccgtcgaag gaccggcccg gctgtcccag cagatagacg
     8221 agccgttcgc gccagctctg cgccgccgcc acatccttgg cgatcgctac ccattcgtgg
     8281 agcgcgatgc gcagcggatt gaagctgccg atgttcttga cgataccgta gcggcacgga
     8341 tcttcgggcg cttcggcgac gaaggtgccg aagagccgat cccagaggat gaagacgccg
     8401 gcatagttgc ggtcgagata gcgcgggttg gtggcgtggt ggacgcggtg gtgggacggc
     8461 gtgttcatca ccgcctcgat ccagagcggc agacggccaa ccgtctcggt gtgaatccag
     8521 aattgataga cgagactcac gccgcgctgg aacacgacga gagccggcgg aaagccgacc
     8581 agcgtcagcg gcagccacag cacccattcg aaaagcccgc cggtccagct ctgccggagc
     8641 gcggtcgaga gattgtaatg ctgcgagctg tgatgattga tatgcgctgc ccaccagaag
     8701 cggcagcgat ggctgacccg gtggaaccag taataggtga ggtcctcggc gaagagaatc
     8761 agcacgaagc tctcccaggc atagccgagc gtgatcaggc ggaactgata gacccagttg
     8821 agcgcgacat aggcgacggc gccccccgcc agaccgatcg caagattgcc gaggcccatg
     8881 gcgagcgatg cggcggtatc gtgcagctca taggcgggaa ggcgccggcg ccaggcaacg
     8941 gcgatctcca gcagaaccag caggacgaaa gcgggaatgg caacggcgac cgggtccgcg
     9001 agcttgggct cgagcgtcat gcgcggcctg ccgaaggcaa aagacgccgc gcccagcgcg
     9061 atgccgcacc cggcagcatc atagcgaagc agcgatgcgt gaaagcgtcc gtatcgaccg
     9121 tcaggcggtc gccatcgacg ccgacgcgcc gcacctcgcc gcgctcgagc gcgcgcacaa
     9181 cgacccggtc gccggcggca aagaccacga cgagcgtccc gtcggccccg gcagcaagcg
     9241 cggtcgtccg accggcgtcg atgacgatct cgtcggcgac aaagtccggc agatcctcgc
     9301 caagccgcgc catcaccagc gcgcgatcga gccgcgcagg gcgcgcgccg gtgagccaca
     9361 cggcggtcag gatgagtgcg atgccgatca gcggaacgag cagagtcagc ggattcgtcg
     9421 ggtccatcgc ggcaatccta atcctgcggc ccggcacgca ccaagcgacg ccaccaatac
     9481 acccatgact tgcagctatc tcgacaacac actaaatttg atatggcaac gcgcggcacc
     9541 cagaaccggt tcgacatctt tcgaggtgac ccatggcacg gaaatccacg ctttgtcgag
     9601 tgacgatgct ggcgacgata cttctgctcg ccggctgcgc ctcgccgcac ggcacgtatt
     9661 ccaacccgta cgggccacgg aatggcgacg cgatgctggt cgacccgcac acgggcgtgg
     9721 tgctcccggg acaatccgat cctggcggag actgacgcgc cggcccgctc ccgcggcgac
     9781 gcaagacgaa gcgacggcgc gacacgcggg gcggcttgcg gcggtcgccg cgatcaagta
     9841 aatccgaatt cgagtccacg tctatcgcgg tggcgcatga cgagaaatgc aacgccctcg
     9901 gcaaagactc ctgtcgcgct cggcgtgacc gtgcttcgcg tgggccgcga ctcgccgcgc
     9961 gggtcccatc cccgctgatt cgcgcagctt gcaatattcc ggggatggcg atgtccgtga
    10021 aattcac
//
LOCUS       DASYNS010000382         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2748164, whole genome shotgun sequence.
ACCESSION   DASYNS010000382 DASYNS010000000
VERSION     DASYNS010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2748164"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..805)
                     /locus_tag="VGP50_11945"
     CDS             complement(185..805)
                     /locus_tag="VGP50_11945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV8028137.1"
                     /translation="MDQVSWLQGNDRSHRQKHRPARSSDHEALLAAVGANRDGEAFAI
                     LFDHFRPRLQIQMVRGGLAPFAAEDVAQDVMETIWRKAHLYDPCKSAAATWVFHIARN
                     RRIDLRRRTREFSVAAEEFLSIADPAAGGETCLDAARRDEQVRTALDALPQEQVTLVR
                     LAFFEGLSHSTIARRLQLPIGTVKSRLRLAFARLRRLLQDVGATGA"
     gene            1069..1734
                     /locus_tag="VGP50_11950"
     CDS             1069..1734
                     /locus_tag="VGP50_11950"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEV8028138.1"
                     /translation="MLLRWTHLRWIDVASLLVAAAFIAPALAQQAAPDAAAPATTPAK
                     KLPKPKPAAAAKPAAGAPAAAAAPPVAGGAKPTLLSQYGEWGAYTAAPGGKKVCFAIA
                     KPSSSETVPSDRPRNPPYMFISTRPAEKVSNEVSIVIGYPFKPNSEATAEVGTTSFAL
                     STQQDGAWVKNATEEAHMVEAMRAGQNAIVKGMSAKGTRSTDTFSLRGLAQALDRVDQ
                     ECK"
     gene            1820..>2262
                     /locus_tag="VGP50_11955"
     CDS             1820..>2262
                     /locus_tag="VGP50_11955"
                     /inference="COORDINATES: protein motif:HMM:NF044960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV8028139.1"
                     /translation="MTTPLAATKPPGSASAAIEKRPLERYVAPEKPSLVGLSRAALAA
                     ALGAIDVPERQRRMRVQQIWHWLYVRGAQDFAAMTTLSKELRLTLAQRFTLARPEIAA
                     EQVSVDGTRKWLIRLPGEVEGRPHEVECVYIPEADRGTLCISSQVG"
BASE COUNT          364 a          758 c          773 g          367 t
ORIGIN      
        1 gtccggcggt cggctgcggg atgtcggcga gctcgagttc gtcgggggtg caatagcggg
       61 tacagaggat ggctttcatg cgggtctcgt catggcggga cgcgcgggac gcgcgagccg
      121 gcaatccata gtcacaaaac accgatgttg gaagccggcg ccggtgcggg gcgggcggcg
      181 cggctcacgc gccggtcgcg ccgacgtcct ggaggaggcg gcgcagccgc gcgaatgcaa
      241 gccgcaggcg cgacttcacc gtcccgatcg gcagctgcag ccgccgcgcg atcgtgctgt
      301 gcgagagccc ctcgaagaag gcgaggcgca ccagcgtgac ctgctcctgc ggcaaggcgt
      361 cgagcgcggt gcgcacctgc tcgtcccgcc gcgccgcgtc gaggcaagtc tcgccgccgg
      421 ccgcggggtc ggcaatgctg aggaattcct cggctgcgac gctgaattcg cgggtgcgtc
      481 gccgcaaatc gatgcggcga ttacgggcga tatggaagac ccaggtggcg gccgccgatt
      541 tgcagggatc atagagatgc gctttgcgcc agatggtttc catcacatcc tgcgccacgt
      601 cctcggcggc aaacggagcg aggccgccgc gcaccatctg tatctgcaag cgcgggcgga
      661 aatggtcgaa caggatggcg aaggcctcgc cgtcacggtt cgcgcccact gcggcgagaa
      721 gagcctcatg gtcgctcgat ctcgccggcc ggtgtttttg ccggtgcgag cgatcatttc
      781 cttgcagcca ggaaacctgg tccacgacac cttctcgccc tggtcagcgc gctcgtaccc
      841 cttgcgtggg gccgaaaggc acgagcagcg cttcgttcac gcagtgcagt atcgagactc
      901 cgacggtgtc gggctgtgct cgccgtcacg ttttgccgcc tgtttgcagg tcgcggcgcg
      961 ctactgtgtc gtttgcgcca tcgcggcttc tgccgatttc gttccctcct cggcagatat
     1021 gggttaaagt tgaagggttt ggattcgcca gcgcgttgac gagggccgat gctccttcgg
     1081 tggacccatc ttcggtggat cgatgtcgct tcgctgctcg ttgccgcggc attcattgct
     1141 ccagcgctcg ctcagcaagc cgcgccggac gccgcggcgc cggcaaccac gcccgcgaag
     1201 aaactcccca agcccaaacc cgctgccgcg gcgaaacctg ccgcgggggc gccggcggcc
     1261 gccgccgcgc cgccggttgc tggcggagca aagcccacgc tgctgagcca gtatggcgag
     1321 tggggcgcct acacggccgc gccgggaggc aaaaaggtct gcttcgcaat cgccaagcct
     1381 tcgtcctcgg agaccgttcc atccgaccgg ccgcgcaacc ctccctatat gttcatttcc
     1441 acgcgcccgg ccgagaaagt gagcaacgag gtgtcgatcg tcatcggcta cccgttcaag
     1501 ccgaattccg aggcgaccgc ggaggtgggc acgacctcgt ttgcgctttc cactcagcaa
     1561 gacggcgcct gggtcaagaa cgcgaccgaa gaggcgcata tggtcgaggc gatgcgcgcc
     1621 ggtcagaacg cgatcgtgaa gggcatgtcg gcgaaaggca cgcgctcgac cgacaccttc
     1681 tcgctgcgcg gcttggccca ggccctcgac cgcgtcgatc aggaatgcaa atagccggac
     1741 ctcggcgtgt ccgctcctgt cgggagggcg gcacgcgccc catctaagct cgaggcgaac
     1801 gcccccaacg gatcggctga tgacgacccc ccttgccgca accaagccgc caggcagtgc
     1861 ttcggccgcg atcgagaaac ggccgctcga gcgctatgtc gcgccggaga agccctcgct
     1921 cgtcggccta tcgcgcgccg cgctcgccgc ggcgctcggc gcgatcgacg tgccggagcg
     1981 gcaacggcgc atgcgcgtgc agcagatctg gcactggctc tacgtgcgcg gcgcgcagga
     2041 tttcgccgcg atgaccacct tgtcgaagga gctgcgcttg acgctagcgc agcgcttcac
     2101 gctggcgcgc ccggagatcg ccgccgaaca ggtctcggtc gacggcacgc gcaaatggct
     2161 gatccgcctt cccggcgagg tcgaaggccg gccgcacgag gtcgagtgcg tgtacattcc
     2221 ggaggccgat cgcggcacgc tgtgcatttc gagccaggtc gg
//
LOCUS       DASYNS010000383         9383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2437925, whole genome shotgun sequence.
ACCESSION   DASYNS010000383 DASYNS010000000
VERSION     DASYNS010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437925"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VGP50_11960"
     CDS             complement(<1..299)
                     /locus_tag="VGP50_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8028140.1"
                     /translation="MLAVSGVHTFYGSIEALRGVDVDVGAEEIVALIGANGAGKSTLL
                     MTICGRPQAARGQIALDGADITRLPTYEIMRRGVAHAPEGRRIFPRMTVLENLQIG"
     gene            complement(490..1296)
                     /locus_tag="VGP50_11965"
     CDS             complement(490..1296)
                     /locus_tag="VGP50_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028141.1"
                     /translation="MRFGGLLAVDDLSFTAPARAITAVIGPNGAGKTTVFNCLTGFYR
                     PSVGRLALHRPDGDFLLERMDGFRIAREAGVARTFQNIRLFAGMTVLENLIVAQHNRL
                     MRQSLYTFGGLIGLPVYRDGERAAVAKATAWLERIGLVDRADWPAASLPYGAQRRLEI
                     ARAMCTDPVLLCLDEPAAGLNARESAELNTLLLEIRDTYGIGLLLIEHDMSVVMGISD
                     HVVVLDYGRKIAAGTPAAVRSDPTVIKAYLGEEEESALPPEIARDLGEMP"
     gene            complement(1341..2654)
                     /gene="livM"
                     /locus_tag="VGP50_11970"
     CDS             complement(1341..2654)
                     /gene="livM"
                     /locus_tag="VGP50_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HEV8028142.1"
                     /translation="MALAAPPARRRALPRLLKDAAAAALVVLALAIPFVGFRAVEVNA
                     ALSIRTRFGAVAVAVAVAFFGRLAIGALSGLRARPAVESRWPTLERRHLAVLAGLGVA
                     FALLLPFLPFASSYVIGIATNVVIYVMLGWGLNVVVGLAGLLDLGYVAFYAVGAYSFA
                     ILAQRFHISFWEGLPIGGILAATFGLILGFPVLRLRGDYLAIVTLGFGEIVHIVLQNW
                     VPLTNGPAGIGNIPPPTLFGLSFTRTAPPGQSTVFQFFHIEFSPLLRLIFVYYIILAL
                     ALITNLFTLRLRKLPIGRAWEALREDEVACRALGINPTTVKLSAFAIGAMLGGFAGTF
                     FAARQNFVSPESFTFTESATILAIVVLGGAGSQLGVVLAAIVLVTLPELGRQFAGVPV
                     IGALGELRTLLFGAAMVGIMIWRPRGLLSRREPSLRLGSGRGGAA"
     gene            complement(2654..3568)
                     /locus_tag="VGP50_11975"
     CDS             complement(2654..3568)
                     /locus_tag="VGP50_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389504.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HEV8028143.1"
                     /translation="MAYFVQQLINGLTLGAIYGLIAIGYTMVYGIIGMINFAHGEIYM
                     IGAFVALITFLVLGALGIGFVPLALLLMLLSAMVFTAVYGWTVERVAYRPLRHSFRLA
                     PLISAIGMSIFLQNYVQLTQGARVKALPPVVTGGFTLMETGGFAVQVSYLQVFIMLLT
                     TVLMIAFSLVIAATPLGRAQRACEQDMTMAALLGIDVDRTISLTFVMGAALAAVAGTM
                     VVLYYGVIDFFIGFLAGIKAFTAAVLGGIGSLPGAMLGGLLIGLIEAFWSGYFSAEYK
                     DVSVFVILVLVLVFRPTGLLGRPDVEKV"
     gene            complement(3684..5408)
                     /locus_tag="VGP50_11980"
     CDS             complement(3684..5408)
                     /locus_tag="VGP50_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV8028144.1"
                     /translation="MTGVAMIRRLRLASGLVMLTYVTTHLLNHALGLVVPVATMDRVL
                     HHVYLVVTDPAVSVLLYGAFALHYSLALWALWQRRSLRMPLADSVQVVLGFLIPFMLV
                     EHVVENRIGDRWFGADHGYYDSLLLDIWVRTPWLGVLQFAVLIVAWVHAMIGLRFWLR
                     LKPWYPRWQPLLYAVALLLPTLALLGFVEAGREVAAAAQDPAWVAALDARHPAAQGAE
                     RLALEQLKIWLLAGFAATLLAVLPAWFVRRAVQRRRGLLRLSYPEGRVVRIVRGLSVL
                     EASRIAGIPHASVCGGRGRCSTCRIRIEGDEDAIPPPAPAELSVLHRIGAGENVRLAC
                     QLRPTGDLRVTPLLPATAQARDGYRRPSYLQGSEREIAILFADLRGFTTLSEHKLPYD
                     LVFLLNRYFAAMGHAVEEAGGRIDKFIGDGVMALFGLDSGAEAGCREALVGARLMAER
                     LAQLNEALVHELPEPLRLGIGVHVGMVIVGEMGYGTAMSVTAVGDTVNTASRLESLTK
                     TYGVELVIADAVARRAGIDLGTAPVHEIEIRGRDERLAVRLIMNAGDLPVSAAVPVQG
                     TLRRAAAD"
     gene            complement(5405..6442)
                     /locus_tag="VGP50_11985"
     CDS             complement(5405..6442)
                     /locus_tag="VGP50_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV8028145.1"
                     /translation="MGETREKKINLALQGGGSHGAFTWGVLDRLLDDERVAIDGISAT
                     SAGAMNGAALCYGYAVGGRVGAKRALAALWRGIASITAPQWAPQTGVQREFSRLWREY
                     LKAMAPFFSAFQRMSYLVSPYEFNPLNINPLKQVLEEAIDFRVLAGGDSAIKLYLSAT
                     NVRTGKIKVFEKKELSADAVLASSCLPLVFHAVEIDGAYYWDGGFMGNPALFPLIYGC
                     DSRDVLVVHINPLERPEIPQSAFEILNRVNEVSFNSSLMREMRAIAFVTKLIDDGTIP
                     AAKAKRMYMHAITADEVLTKLGMVTMMNTDWDFLQDLRDIGRRYAERWLETTWDRIGK
                     ESTIEIEALYM"
     gene            6669..7778
                     /locus_tag="VGP50_11990"
     CDS             6669..7778
                     /locus_tag="VGP50_11990"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV8028146.1"
                     /translation="MHAIRIDRMKPLAQEPHSGHNRYHPEIEPALEVGEGEEVVLETR
                     DGLDGQITPSSTAADFATLNVGAVHPLTGPVFVKGAKPGDMIEIEFLDIVPQPTAFSA
                     IVPGLGFLRDVMTEPFLVHWKIRDGWATSDQIPRVRVPGAPFMGVSATAPSAQKLKEW
                     TAREQRVIDKGGMALPPDPAGAVPAGACGLTGLRTLPPRENGGNFDVKQLTKGAKLYL
                     PVFQEGALFSTGDGHFAQGDGEVCVTAVEMGATVAVRFRLQKGAAAARKFMAPVFTRQ
                     SYFADPKFAAPERFLGVMGMPINAEGEIEAENLTLACRNAVLNMMELLQERGFTRQQA
                     YVICSVAVDLRISNVVDVPNYVVSALLPEGIFSGD"
     gene            complement(7806..>9383)
                     /gene="glgX"
                     /locus_tag="VGP50_11995"
     CDS             complement(7806..>9383)
                     /gene="glgX"
                     /locus_tag="VGP50_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEV8028147.1"
                     /translation="FFAPEPRYMASASVNEVKEVIARFHDAGIEVILDVVYNHTAEGN
                     EKGPTLCFKGIDNATYYRLLPEKRHYINDTGTGNTLNLSHQRVLQMVTDSLRYWAQEM
                     RVDGFRFDLATILGREPYGFDEGGGFLDSCRQDPVLSGVKLIAEPWDCGPGGYQVGGF
                     PPGWAEWNDRFRDTLRAYWKGDDGKLPDLAARLTASSDLFNKRGRKSWAGVNFVTAHD
                     GFTLNDLVSYNDKHNEANGEENRDGNTNNLSWNCGVEGATDDPQIRGLRERQKRNLLA
                     TLLLSQGTPMILGGDEFGRSQGGNNNAYCQDNETTWFDWEGIGEEGKALIDFVRRLIV
                     IRRTFPVLRRGRFFTGALDETLGIKDVTWLTPSATEMAPEQWQDANAKCLGVIFDGRA
                     QATGIRRPASDATLLLVVNSYHDVVLFKLPDVAGGARWELLIDTNLPEQAEIAPFAFG
                     HDYEVTGRSLLLFALKSEVPRGIIRRAQDALKTMAEQPVATPVARRLPEPKPKETERA
                     EEHQPAEAEPQEVEPVS"
BASE COUNT         1678 a         3091 c         3222 g         1392 t
ORIGIN      
        1 ccgatctgca ggttctcgag gaccgtcatg cgcggaaaga tgcgccggcc ttccggcgca
       61 tgcgcgacgc cgcgccgcat gatctcgtag gtcggcagcc gcgtgatgtc cgcgccgtcg
      121 agcgcgatct gcccgcgcgc cgcctgcgga cgcccgcaga tggtcatgag cagcgtcgac
      181 ttcccggcgc cattggcgcc gatcagcgcg acgatttcct cggcgccgac gtcgacatcg
      241 acgccgcgca gcgcctcgat gctgccgtag aatgtgtgta ccccgctgac cgcgagcagc
      301 gccatggcta agcctgcttc cccctggagc cctctccgcc cgcttgcggg gggagagggc
      361 gcgagcgaag cgagcgggtg aggtgggtgc cgatggcggg cccggtgccg tcgcggcgag
      421 acacctcacc ctccccgtcg ctatcgcgac gggcccccac cctctccccg cgtcgcgcgg
      481 agagggacat caaggcatct ccccgagatc gcgcgcgatc tccggcggca gcgcgctttc
      541 ttcctcttcg ccgagatagg ccttgatcac ggtgggatcg ctgcgcacgg cggcgggcgt
      601 gccggcggcg atcttgcggc cgtagtcgag cacgacgaca tggtcggaga tacccatgac
      661 cacgctcatg tcgtgctcga tcagcagaag cccgatgcca taggtgtcgc ggatctcgag
      721 cagcagcgtg ttgagctcgg ccgattcgcg cgcattgaga ccggccgccg gctcgtcgag
      781 acacagcagg accgggtcgg tgcacatggc gcgcgcgatc tcgagccggc gctgcgcgcc
      841 atagggcagg ctcgcggccg gccaatcggc gcgatcgacg aggccgatgc gctcgagcca
      901 ggccgtcgcc ttcgccacgg cggcgcgctc gccgtcgcga tagacgggca gcccgatcag
      961 tccgccgaag gtatagagac tctggcgcat caggcgattg tgctgcgcga cgatgaggtt
     1021 ctccagcacc gtcatgccgg cgaagagacg gatgttctgg aaggtgcgcg ccacgcccgc
     1081 ctcgcgcgcg atgcggaaac cgtccatgcg ttcgagaagg aagtcgccat ccggacgatg
     1141 cagcgcaagc cgtccgacgc tcggccgata gaatccggtg agacagttga acacggtcgt
     1201 cttgccggcg ccgttggggc cgatgacggc ggtaatggcg cgcgccggcg ccgtgaagga
     1261 cagatcgtcg accgcgagca ggccgccgaa gcgcatgctc agatgctcga cttcgagcag
     1321 cggcggcgcc gctcgcgtcg tcacgcggct cctccgcgcc cgctgccgag ccggagcgag
     1381 ggctcgcgtc gcgacaggag gccgcgtggc cgccagatca tgatgccgac catcgccgcg
     1441 ccgaacagca gggtgcgcag ttcgccgagc gcgccgatca ccggcacgcc ggcgaattgc
     1501 cgcccgagct cgggcagcgt taccagcacg atcgccgcaa gcacgacgcc gagctggctc
     1561 ccggcgccgc cgagcacgac gatggcgagg atcgtcgcgc tctcggtgaa tgtgaagctt
     1621 tccgggctga cgaagttctg ccgcgcggca aagaacgtgc cggcgaagcc gccgagcatg
     1681 gcgccgatgg cgaaagccga gagcttgacc gtcgtcggat tgatgccaag cgcgcggcac
     1741 gccacctcgt cctcgcgcaa cgcttcccag gcgcgtccga tcggcagctt gcgcagccgc
     1801 aaggtgaaga gattcgtaat cagcgccagc gcgaggatga tgtagtagac gaagatgagc
     1861 cgcaacagcg ggctgaactc gatgtgaaag aactggaaga ccgtgctctg tcctggcggc
     1921 gcggtccggg tgaagctcag gccgaagagc gtcggcggcg ggatgttgcc gatgccggcg
     1981 ggcccattgg tcaggggcac ccagttctgc aggacgatat ggacgatctc gccgaagccg
     2041 agcgtgacga tggcgagata gtcgccgcgc agccgcagca cgggaaatcc caggatgagc
     2101 ccgaaggtcg cggcgagaat gccgccgatg ggcagccctt cccagaagga gatatggaag
     2161 cgctgagcga ggatcgcgaa gctgtaggcg ccgacggcat aaaaagcgac ataaccgaga
     2221 tcgagcaatc cggcgaggcc gacgacgaca ttgaggcccc agcccagcat cacgtagatc
     2281 acgacattgg tggcgatgcc gatcacatag ctcgaggcga agggcaggaa ggggagcagg
     2341 agggcaaagg caacgccgag cccggcgagg acggccaggt gtcgccgctc gagcgtcggc
     2401 cagcgactct cgaccgccgg gcgggctcgg aggccgctga gcgcgccgat cgccaggcgg
     2461 ccgaagaagg ccacggcgac ggcgacggcc acggcgccga accgcgtgcg aatactgagc
     2521 gcggcgttga cttcgacggc ccggaagccg acgaacggaa tggcgagcgc cagtacgacc
     2581 agcgccgctg cggctgcgtc cttcagaagt cgtggcaacg cgcggcgacg cgccggcggc
     2641 gccgcgagcg ccatcagact ttctccacgt ccggccgccc gagaaggccg gtcgggcgga
     2701 agacgagcac gagaacgagg atgacaaaga ccgagacgtc cttgtactcg gccgagaaat
     2761 agcccgacca gaaggcttcg atgaggccga tgaggaggcc gcccagcatg gcgccgggca
     2821 aggacccgat gccgcccagc acggccgcgg tgaacgcctt aatgccggcg aggaagccga
     2881 tgaagaaatc gatcacgccg taatagagca cgaccatcgt gccggcgacg gcagcgagcg
     2941 ccgcgcccat gacgaaggtg agcgagatcg tgcggtcgac atcgatgccc agcagcgccg
     3001 ccatggtcat gtcctgctcg caggcgcgct gggcgcggcc cagcggcgtg gcggcgatga
     3061 cgaggctgaa cgcgatcatc aacacggtcg tcagcagcat gatgaagacc tggagatagc
     3121 tcacctgcac agcgaatccg ccggtctcca tcagcgtgaa gccgcccgtt accaccggcg
     3181 gcagcgcctt gacgcgcgcg ccctgcgtca gctgcacata gttctgcagg aagatcgaca
     3241 tgccgatggc ggagatgagc ggcgccaggc ggaaggaatg gcgcagcggc cggtaggcca
     3301 cccgctccac cgtccagcca tagacggcgg tgaagaccat tgccgagagc agcatcagga
     3361 gcagcgccag cggcacgaag ccgatgccga gcgcgcccag gacgaggaag gtgatgagcg
     3421 ccacgaaggc gccgatcatg tagatctcgc catgggcgaa gttgatcatg ccgatgatgc
     3481 catagaccat cgtgtagccg atggcgatga ggccatagat cgcgccgagc gtcaggccgt
     3541 tgatcaactg ctgaacgaaa tacgccatgc cccctcgccc ccggacgcgc ccaaggtaag
     3601 cgccaccgct gcggcgggca agccaggggc tgcgacggag cgaagacggc ccacggtcgg
     3661 gcgcccgaaa cttgcttcga tcctcagtcc gccgctgcgc ggcggagggt gccctgcacc
     3721 ggcaccgcgg cggacaccgg cagatcgccc gcattcatga tcagccgcac ggcaagccgc
     3781 tcgtcccggc cacggatctc gatctcgtgg accggcgccg tgccgagatc gatgccggca
     3841 cgccgcgcca ccgcatcggc gatgacgagc tcgacgccat aggtcttggt cagactttcg
     3901 agccggctcg ccgtgttgac ggtatcgccg accgcggtca cggacatggc cgtgccgtag
     3961 cccatctcgc cgacgatgac catgccgaca tggacgccga tgcccaggcg caacggctcc
     4021 ggcagctcat ggaccagcgc ttcgttcagt tgcgcgagcc gctccgccat cagtcgcgcc
     4081 ccgaccagcg cctcgcggca cccggcttcg gcgccgctgt cgagcccgaa cagcgccatg
     4141 acgccgtcgc caatgaattt gtcgatacgc ccgcccgcct cttcgacggc atgccccatc
     4201 gcggcgaaat atcggttgag caggaagacc aggtcatagg gcagcttatg ctccgagagc
     4261 gtcgtgaagc cgcggagatc cgcgaaaagg atcgcgattt cgcgctcgct gccctggaga
     4321 tagctcggcc ggcgataacc gtcgcgcgcc tgggccgttg ccggcagcag cggcgtcacg
     4381 cggagatcgc cggtggggcg aagctggcag gcgagacgga cattctcgcc cgcgccgatg
     4441 cggtggagaa cgctcagctc cgcgggcgcc ggcggcggga tcgcatcctc gtcgccttcg
     4501 atgcggatgc gacaggtgct gcaacggccg cggccgccgc agacggaggc gtgcggaatt
     4561 ccggcgatcc ggctcgcctc cagaaccgac aggccgcgca cgatcctgac gacgcggcct
     4621 tcgggataag acaggcgcaa aagtccgcgc cggcgctgga cggcgcgccg gacgaaccac
     4681 gccggcagca cggccagcag agtcgcggcg aagcccgcga gcagccagat cttgagctgc
     4741 tcgagcgcta gcctctcggc tccttgcgcc gccggatgcc gggcatcgag cgctgccacc
     4801 caggccggat cctgtgccgc cgccgccacc tcgcgcccgg cctcgacaaa gccgagaagc
     4861 gccagcgtcg gcagcagcag agcgaccgca tagagcagcg gctgccagcg cgggtaccag
     4921 ggcttcaagc gcagccagaa gcgcagcccg atcatcgcgt gcacccaggc gacgatgagc
     4981 acggcgaatt gcaacacgcc gagccagggc gtgcgtaccc agatgtcgag cagcagcgag
     5041 tcgtaatagc cgtggtcggc gccgaaccag cggtcgccga tgcggttctc gaccacatgc
     5101 tcgaccagca tgaagggaat gagaaatccg agcacgacct ggacgctgtc ggcgagcggc
     5161 atgcggagcg agcgccgctg ccacaaggcc cagagcgcca ggctgtaatg gagagcgaac
     5221 gcgccgtaga ggaggaccga caccgcggga tcggtgacga cgagatagac gtgatggagc
     5281 acgcggtcca tggtcgcgac cggcaccacg agccccagcg cgtggttgag cagatgggtg
     5341 gtcacatagg tgagcatgac cagccccgag gcgaggcgga gccgtcggat catcgcgacg
     5401 ccggtcacat gtagagcgcc tctatttcga tcgtcgattc cttgccgatg cgatcccagg
     5461 tcgtctcgag ccagcgctcg gcatagcgcc ggccgatgtc gcgcaggtcc tggaggaaat
     5521 cccagtccgt gttcatcatg gtgaccatgc cgagcttggt cagcacctcg tcggcggtga
     5581 tggcatgcat atacatgcgc ttcgccttgg cggcggggat ggtgccgtca tcgatgagct
     5641 tggtgacgaa ggcgatcgcg cgcatctcgc gcatgagcga ggagttgaag ctgacctcgt
     5701 tgacgcggtt caggatctcg aaggccgatt gcgggatctc cgggcgctcg agcggattga
     5761 tgtgaacgac gaggacgtcg cggctgtcgc agccatagat cagcgggaag agcgccggat
     5821 tgcccatgaa gccgccatcc cagtaatacg cgccgtcgat ctcgacggcg tggaaaacca
     5881 gcggcaggca ggacgaggcc agcacggcgt cggccgagag ctccttcttc tcgaagacct
     5941 tgatcttgcc ggtgcgcaca ttggtggcgg aaagatagag cttgatcgcg gaatcgccgc
     6001 cggcgaggac gcggaaatcg atggcttcct cgagcacctg cttcagcggg ttgatgttga
     6061 gcggattgaa ctcgtagggc gagaccagat aagacatgcg ctgaaaggcc gagaagaacg
     6121 gcgccatcgc cttcaggtat tcgcgccaga gcctgctgaa ttccctctgg accccggtct
     6181 gcggcgccca ttgcggggcg gtgatgctgg cgatgccccg ccagagcgcg gcgagcgcgc
     6241 gcttcgcgcc gacgcggccg ccgactgcat agccatagca aagggcggcg ccgttcatgg
     6301 cgccggcact ggtcgcagaa atcccgtcga ttgcgacacg ctcgtcatcg agcagccggt
     6361 cgagcacgcc ccaggtgaag gcgccatgcg agccccctcc ctgaagcgcg agattgattt
     6421 ttttctctct ggtctcgccc atctcgcgcc ccgtcgcagc gcgccccagc gctccggcaa
     6481 agcaatcctg catatttccg gcggattgac aatgcagttg ccgcggccgg gccggacggt
     6541 gcgaggggcc gggcgcaata cgtgatccgt cggattgcgc gggacgggcc attggctcag
     6601 actggtcggt ctcgcaaggc cgaaccaccc aagggggcag tacgacgcat cggaggaggg
     6661 agtcccgcat gcatgcgatc cgcattgatc gaatgaagcc gctggcccag gagccgcatt
     6721 cgggccacaa ccggtatcac cccgagatcg agccggcgct cgaggtcggc gaaggcgaag
     6781 aggtcgtcct cgagacgcgc gacggactgg acggccagat cacgccgagc tcgaccgcgg
     6841 ccgatttcgc gacgctcaac gtcggcgccg tccatcccct gaccggcccc gtcttcgtca
     6901 agggcgccaa gcccggcgac atgatcgaga tcgagttcct cgacatcgtt ccccagccga
     6961 ccgccttcag cgccatcgtt ccgggcctgg ggttcctgcg cgacgtcatg accgagccct
     7021 tcctcgtgca ctggaaaatc cgcgacggct gggcgacgtc ggaccagatc ccacgcgtcc
     7081 gcgtccccgg cgcgcccttc atgggcgtct ccgccaccgc tccttccgcg cagaaactca
     7141 aggaatggac ggcccgcgag cagcgcgtca tcgacaaggg cggcatggcg ctgccgcccg
     7201 atcccgccgg cgccgttccg gccggtgcgt gcgggctcac cggcctccgc acgctgccgc
     7261 cccgcgagaa tggcggcaac ttcgacgtga agcagttgac caagggcgcg aagctctacc
     7321 tgcccgtctt ccaggaaggc gcgctgttct cgaccggcga tgggcatttc gcccaaggcg
     7381 atggcgaggt ctgcgtcacg gcggtcgaga tgggcgccac cgtggccgtg cgcttccggc
     7441 tgcagaaagg cgccgcggcg gcgcggaaat tcatggcgcc ggtcttcacg cggcagagct
     7501 acttcgccga tcccaaattc gccgcccccg agcgcttcct tggagtgatg ggcatgccga
     7561 tcaatgcgga aggcgagatc gaggcggaaa acctgacgct cgcctgccgc aacgccgtgc
     7621 tcaacatgat ggagctgctg caggagcgcg gattcacccg ccagcaggcc tatgtcatct
     7681 gcagcgtcgc cgtggacctg cgcatctcga acgtcgtcga cgtcccgaac tacgtcgtgt
     7741 cggcgctgct gccagagggg atcttcagcg gcgactgagg gctggcgtcg tggcggcgcg
     7801 cggcgtcacg acaccggctc gacctcctgc ggctccgcct ctgccggctg gtgttcttcc
     7861 gcgcgttccg tctctttcgg cttcggctcg ggcaggcggc gcgcgacggg cgtggcgacc
     7921 ggctgctcgg ccatggtctt gagcgcatcc tgggcgcgac ggatgatgcc gcgcggcacc
     7981 tccgacttga gggcgaagag cagcagcgag cggcccgtga cctcgtagtc gtgaccgaag
     8041 gcgaagggag cgatctcggc ctgctccggg agattggtgt cgatcagcag ctcccagcgc
     8101 gcaccacccg cgacgtccgg caatttgaac aacaccacgt cgtgatagct gttgacgacg
     8161 agcagcagcg tcgcgtccga cgccggacgg cggatgccgg tcgcctgcgc acggccgtcg
     8221 aagatcacgc cgagacactt ggcattggcg tcctgccatt gctcgggcgc catctcggtg
     8281 gctgacggcg tgagccacgt cacgtccttg atgccgagcg tttcgtccaa agcaccggtg
     8341 aagaagcggc cgcggcgcag caccgggaag gtgcggcgga tgacgatgag cctgcgcacg
     8401 aagtcgatga gcgccttgcc ctcctcgccg atgccttccc aatcgaacca ggtggtctcg
     8461 ttgtcctgac aataggcgtt gttgttgccg ccctggctgc ggccgaactc gtcgccgccg
     8521 aggatcatcg gcgtgccttg cgagagcagc agcgtcgcca gcagattgcg cttctgccgc
     8581 tcgcgcaagc cgcggatctg cggatcgtcc gtcgcgccct caacgccgca attccacgag
     8641 agattgttgg tgttgccgtc gcggttctct tcgccattcg cctcgttgtg cttgtcgttg
     8701 taggagacga ggtcgttgag cgtgaagccg tcatgggcgg tgacgaagtt gacgccggcc
     8761 caggacttgc gcccgcgctt gttgaagagg tcgctcgagg cggtcagacg cgcggcgagg
     8821 tcgggcagct tgccgtcatc gcccttccaa taggcgcgca gggtgtcgcg gaagcggtcg
     8881 ttccactccg cccagcccgg cgggaagccg ccgacctggt agccgccggg gccgcaatcc
     8941 cagggctcgg cgatgagctt cacgcccgag agcacggggt cctggcggca cgagtcgaga
     9001 aagccgccgc cttcgtcgaa accatagggc tcacgtccga gaatcgtcgc aaggtcgaag
     9061 cggaagccgt cgacgcgcat ctcttgcgcc cagtagcgaa gactgtcggt caccatctgc
     9121 aagacgcgct gatgactgag attgagcgtg ttgccggtgc cggtgtcgtt gatgtagtgg
     9181 cgcttttcgg gcagcagccg gtagtaggtc gcgttgtcga tgcccttgaa gcagagcgtc
     9241 ggccccttct cgttgccttc ggcggtatgg ttgtagacga catcgaggat cacctcgatg
     9301 cccgcgtcgt ggaaacgcgc gatcacttcc ttcacttcgt tgacggaggc actcgccatg
     9361 tagcgcggct cgggcgcgaa gaa
//
LOCUS       DASYNS010000384        10117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1501477, whole genome shotgun sequence.
ACCESSION   DASYNS010000384 DASYNS010000000
VERSION     DASYNS010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1501477"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1133
                     /locus_tag="VGP50_12000"
     CDS             225..1133
                     /locus_tag="VGP50_12000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEV8028148.1"
                     /translation="MRVLVIKLGALGDFVQALGPAAAIRAHHGDAEITLLTTAPYREL
                     ALAAPYFDRVWVDERPGFFAPLGLLRLRRRLREGRFGRVYDLQTSTRSSFYHLLMGRP
                     QWSGIARGASHPHRGAERDRMHTLDRQAEQLRLAGITSVPLPDVSWAARDIGPFGLSG
                     RLLLLVPGGAPHRPEKRWPVDHFAALAVLAARRRMTPVVIGGTAEATLGAVIAERCST
                     ARDLTGRTSFGEIVGLARMAQHAVGNDTGPMHLITAAGCASSVLYSAASDPALTAPRG
                     ASVTILRRATLDSLAVEEVAATLPVG"
     gene            1158..2852
                     /gene="thrS"
                     /locus_tag="VGP50_12005"
                     /pseudo
     CDS             1158..2852
                     /gene="thrS"
                     /locus_tag="VGP50_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
     gene            2874..3818
                     /gene="thrS"
                     /locus_tag="VGP50_12010"
                     /pseudo
     CDS             2874..3818
                     /gene="thrS"
                     /locus_tag="VGP50_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590802.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
     gene            3926..4441
                     /gene="infC"
                     /locus_tag="VGP50_12015"
     CDS             3926..4441
                     /gene="infC"
                     /locus_tag="VGP50_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV8028149.1"
                     /translation="MTPSRDGPRVNQEIVVPRVRLVDERGEMIGVVTRTEALQRATEA
                     GLDLVEVAATADPPVVKILDYGKFKYEEQKRKNEARKKQKVIEVKEIKLRPGIDDHDY
                     EVKMRSMVKFIEEGDKVKVTMRFRGRELAHQEIGMNVLMRVKGDLDEVAKVEQHPRME
                     GRQMTMVMSPK"
     gene            4712..5269
                     /locus_tag="VGP50_12020"
     CDS             4712..5269
                     /locus_tag="VGP50_12020"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEV8028150.1"
                     /translation="MKGHILAAAALAAGSLAMSVAPAKAQEFTATFSGFQETGALNNE
                     SGSILSNGTAKLRLNVNKVAQTATFTLTFSGLSTPITQSHIHFGRVHTPGNVIVFFCS
                     NLNNGPAGTQLCPQNATSGTVTGTITAADIIAVPSQGVPADNFDALTDALNSNTAYGN
                     VHTTQFPNGEIRGQVLVGELGDFEN"
     gene            5494..5697
                     /gene="rpmI"
                     /locus_tag="VGP50_12025"
     CDS             5494..5697
                     /gene="rpmI"
                     /locus_tag="VGP50_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014873788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV8028151.1"
                     /translation="MPKMKTKSGAKKRFSRTATGKIKANVAHKRHRLISKPKKMKRKA
                     RGTFVLEKGDARLVLTYMPYLRG"
     gene            5701..6072
                     /gene="rplT"
                     /locus_tag="VGP50_12030"
     CDS             5701..6072
                     /gene="rplT"
                     /locus_tag="VGP50_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV8028152.1"
                     /translation="MARVKRGVTTHARHKKVLGLAKGYRGRAKNAFRIAIEKVEKGLR
                     YAYRDRRAKKRNFRALWIQRINAGARAEGLTYSEFINGMKRAGIELDRKVLADLAVRE
                     PEAFKAIVAQAQAALSQAAPA"
     gene            6193..7269
                     /gene="pheS"
                     /locus_tag="VGP50_12035"
     CDS             6193..7269
                     /gene="pheS"
                     /locus_tag="VGP50_12035"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV8028153.1"
                     /translation="METLDHIRDEILGLIKAATSRDALEQARVALLGKKGRVTALMKG
                     LAGLDPDARRQQGAALNRLKDEVEEAIGHAARRLESEALTQRLAGERLDVTLPVAAES
                     QGRIHPLSQTIDELVAIFGEMGFVVAEGPDIEDDFHNFTALNIPPEHPARQEQDTFYL
                     KAASESGRKVLRTHTSPVQVRTMLAQKPPIRVICPGRTFRSDHDATHSPMFHQVEGLV
                     IDQTTHMGHLRGCLVEFCRAFFDVDDLPLRFRPSYFPFTEPSAEVDIGCSRAGGELKI
                     GAFGDWLEILGCGMVNPQVLRNCGIDPAIYQGFAFGVGIERIAMLKYGIPDLRTFYES
                     DLRWLRHYGFLPLDVPSMSGGLAR"
     gene            7266..9662
                     /gene="pheT"
                     /locus_tag="VGP50_12040"
     CDS             7266..9662
                     /gene="pheT"
                     /locus_tag="VGP50_12040"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV8028154.1"
                     /translation="MKTTLAWLKTHLETTASLDAIVEKLVMLGLEVDAVENRGKALAP
                     FVVAQVLSAESHPNADKLRVCMVDTGSERLQVVCGAPNARAGMRGVFAPAGTVIPRSG
                     VVLKESMIRGVASKGMLCSGYELGLSEDHEGIIELAADAPVGASFAQLMGLDDAVLDI
                     KVTPNRGDCLGVRGIARDLAASGIGRLLPLDATPVPGRFPSPVAVHLAGPDDKACPLF
                     IGRMIRGLRNGPSPRWLQDRLTAIGLRPISALVDITNFLTFDAGRPLHVFDADKIGGD
                     LVVRGARPGETLPALNGKTYALDPEMAVIADDDEVLSLGGVIGGASSGCSDATTDVFI
                     EAALFDPVRTAATGRKLQLQSDARYRFERGVDPEFTRPGLEIATRMILELCGGEPGEV
                     TTAGAVPKPIPALAFPPALVRSLGGVDVPTGECVGILGALGFVSEAQAGALLVRVPSW
                     RHDIAGNADLVEEILRVHGYDHIPAVPLLRETPLPKPALTPAQRRTGFVRRSLAARGL
                     VEAVTFSFMHGKIAELFGQVPDTLRLANPISADLDVMRPSILPNLLGAAQRNADRGFP
                     DGALFEVGPQYRDDTPEGQDLMAAGMRSGRTGPKRWDDPGHPVDAMMAKADALAAVAA
                     SGLAPESVQVSADPPDWYHPGRAGTLRLGPKVLGYFGELHPGVLEEMDVTGPAVGFEI
                     FLGHVPLPRAKQGRSRPPLKLSPLQPVERDFAFVVPAELPAETLLRAARTVDKRLVRD
                     VRLFDVYHGPGVGEGRKSLAITVVLQPEEATLTDKEIEAFSEKLIAQVEKATGGSLRR
                     "
     gene            9668..>10117
                     /locus_tag="VGP50_12045"
     CDS             9668..>10117
                     /locus_tag="VGP50_12045"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV8028155.1"
                     /translation="MVAFAKIRKAAEKRLGADGVAARLPPVKSPAQLKKLGDDRYLSL
                     MSLRIFRAGLKHEMVDAKWPAFETAFQGFNPKRVRAMSDEQVEALMKDAQLIRHLGKL
                     KAVHANAATMCTLAEEHGSFAAWLAAWPASEITKLWDALAKRFAQLGG"
BASE COUNT         1690 a         3437 c         3320 g         1670 t
ORIGIN      
        1 cccggagcca cggaacagtc gcaagatgaa ccacagaggc acagaggcac agagaagaaa
       61 gaatcaccac agaggcggtg aggcagggag gggtgagaaa ggcgcgcttc gcgcgtccga
      121 tttcttcttc tcacggtctc actgtctctg tggtgaatct tgtttttctt ctctgcgcct
      181 ctgtgcctct gtggtcaatc tttgaagccg cgcgcttagg cgtgatgcgg gttctcgtca
      241 tcaagctggg ggcgctcggc gatttcgtgc aggcgctggg gccggcggcg gcgatccggg
      301 cccatcatgg cgatgccgag atcacgctgc tgacgaccgc gccttaccgg gagctggcgc
      361 tggccgcgcc ctatttcgat cgcgtctggg tcgacgagcg gcccggcttc ttcgcgccgc
      421 tcggtctgct gcggctgcgg cggcgcttgc gggagggccg cttcggccgc gtctacgatc
      481 tccagacctc gacccgctcg agtttctatc atctcctgat ggggcggccg caatggtcag
      541 ggatcgctcg cggtgcgtct catccccatc ggggcgccga gcgcgaccgc atgcatacgc
      601 tcgaccgcca ggccgagcag ctgcgcctgg ccggcatcac gagcgtgccg cttcccgacg
      661 tctcctgggc agcgcgcgac atcggtcctt tcggcctctc cgggcggctt ctgctgctgg
      721 tgcctggcgg cgcgccgcat cgcccggaga aacgctggcc ggtcgatcat ttcgcggccc
      781 tggcggtgct ggcggcacgc cgccgcatga cccccgtggt gatcggcggc acagcggagg
      841 cgacgcttgg ggcggtgatt gcggagcgct gctccaccgc ccgcgatctc accgggcgca
      901 ccagcttcgg cgagatcgtc ggtctcgcgc ggatggcgca gcatgccgtc ggcaacgaca
      961 ccgggccgat gcatctcatc actgccgccg gctgcgcctc gagcgtgctt tattcggcgg
     1021 cgtcggaccc ggccttgacc gcgccgcgcg gtgcttcggt caccattctc cggcgcgcga
     1081 cgctcgacag ccttgcggtg gaggaggttg ccgccaccct gccggtcggg taaacccctc
     1141 accatatgaa agcgcagatg tccccgtccg tcgccatcac gctccccgac ggcaagcagc
     1201 gccatttccc cggcgcggtg accggcgccg agatcgcgca ggcgatcggg cccgggcttg
     1261 ccaaggcggc gctcgccgtc atggtcgatg gcgagccgaa ggacttggcg acacgcatcg
     1321 accacgacgc caagatcgcc atcctgacgc gcgactcgcc ccaggcgctc gagattctgc
     1381 gccacgacgc ggcgcatgtg atggcagagg ccgtcaagga gctctatccc gacgcccagg
     1441 tcaccttcgg cccggcgacc gagagcggct tctactacga tttcgcccgc gaccagccct
     1501 tcacgcccga ggatctcgcg cggatcgagc tgcgcatgca cgcgatcgtc gaccgcgacg
     1561 agccgatcgc gcgcgaggag tgggaacgcg accgcgccgt cgccttcttc cagtcgatcg
     1621 gcgaaaagta caaagcggag tggatcaacg agatcccgcg cgacgaggcg atcagccttt
     1681 atcgccaagg cggcttcatc gatctctgca ccgggccgca tctgccctcg accggcaagc
     1741 tcggccacgc cttcaagctg atgaaggtgg ccggcgccta ttggcgcggc gatgcgaaga
     1801 accagcagct ccagcgcatc tacggcacgg cctggcccga cgagaaagcg ctgaagcagt
     1861 atctcttcca gctcgaggag gcggagaagc gcgaccatcg ccgcctcggc cgcgagctcg
     1921 acctcttcca ccagcaggaa gaggcggtgg gcagcgtgtt ctggcatccc aaggggtgga
     1981 cgctctatcg cgtcatcgag agctatctgc gcgaccgcct ggacaaggcc ggctatctcg
     2041 agatcaagac gccgcagctc ctcgaccgct cgctctggga agcgtcgggc cattgggaga
     2101 agttccgcca cgtcatgttc acggtgcggg acgaggagga ggggaaagag cgcgtcctcg
     2161 ccgtcaagcc gatgagctgc cccggccatg tgcaggtctt ccgccagcat ctgcacagct
     2221 accgcgagct gccgctgcgc ttcgccgagt tcggctcctg tcatcgcaac gagccgtcgg
     2281 gcgcgctcca cggcatcatg cgcgtgcgtg ccttcaccca ggacgatgcg catatcttct
     2341 gcaccgaggc gcagatcact tcggagacgg tggcgttctg cgagctgctg cgctccgtct
     2401 accgcgcctt cgggttcgac gcgttcatga tcaaattcgc cgaccggccg gcgctgcgcg
     2461 ccggcacgga cgaggcctgg gaccgcatgg agggcgccct caaagcggcg accgaagcgg
     2521 ccggcctcag ctatacgctc aatcccggcg agggcgcctt ctacggaccc aagctcgaat
     2581 tcgtgctgcg cgacgccatc ggccgcgact ggcaatgcgg caccctgcag gtcgatttcg
     2641 tgctgcccga gcggctcgag gccagctata tcggcgagga cgggcagcgc catacgccgg
     2701 tcatgctgca ccgcgccatc ttcggctcga tggagcgctt catcgccatc ctcatcgagc
     2761 attatgccgg gcgcttcccg ctgtggctgg cgccggtcca ggcggtggtg gtcaccatca
     2821 cctccgatgc cgatgcctat gccgagacgg tgcgggacga ggaggagggg acagagcgcg
     2881 tccttgccgt caagccgatg agctgccccg gccatgtgca ggtcttccgc cagcatctgc
     2941 acagctaccg cgagctgccg ctgcgcttcg ccgagttcgg ctcctgtcat cgcaacgagc
     3001 cttcgggcgc gctccacggc atcatgcgcg tgcgtgcgtt cactcaggac gatgcgcata
     3061 tcttctgcac cgatgcgcag atcacctcgg agacggtggc gttctgcgag ctgctgcgct
     3121 ccgtctatcg cgcgttcgga ttcgacgaat tcatggtcaa attcgccgac cggccgacgt
     3181 tgcgcgccgg cacggacgag gcctgggacc gcatggaggg cgccctcaaa gcggcgaccg
     3241 aagcggccgg cctcagctat acgctcaatc ccggcgaggg cgccttctat ggacccaagc
     3301 tcgaattcgt cctgcgcgac gccatcggcc gcgactggca atgcggcacc ttgcaggtcg
     3361 atttcgtgct gcccgagcgg ctcgaagcga gctatatcgg cgaggacggg cagcgccata
     3421 cgcccgtcat gctgcaccgc gccatcttcg gctcgatgga gcgcttcatt gccatcctca
     3481 tcgagcatta tgccgggcgc tttccgctgt ggctggcgcc ggtccaagcg gtggtggtca
     3541 ccatcacctc cgacggcgat tcctatgccg agacggtgcg ggaggcctgc gcccgcgccg
     3601 gcctgcgggt cgaggccgat ctccgcaacg agaagatcaa ctacaagatc cgcgagcaca
     3661 gcctggccaa ggttccggcg ctgctggtgg tcggcaagcg cgaggcggag cagggcacgg
     3721 tcgcgctgcg ccgcctggcc ggcaaggacc aagaaattct tgcgctcggc gaggccgtcg
     3781 ctagattgaa agaggaagcg gcggtcccgt cgccttgagc gcgggcgccg ctgggccttg
     3841 acggtaccgg cgagcacggg ccggcgccgc gggtccgctg tgcattttag agaggaggag
     3901 ctacatcgct agactacccc agaatgtgac gccgagccgc gacggcccgc gcgtcaatca
     3961 agagatcgtg gtgccgcgcg tccgcctggt cgacgaacgc ggcgaaatga tcggagtcgt
     4021 tacccggacc gaggcgctgc agcgggcgac cgaggcagga ctcgatctgg tcgaagtcgc
     4081 cgccactgcc gacccgccgg tggtcaagat cctcgattac ggcaagttca aatacgagga
     4141 gcagaagcgg aagaacgaag cgcgcaagaa gcagaaggtc atcgaggtca aggagatcaa
     4201 gctccggccg ggcatcgacg accatgacta cgaagtcaag atgcgcagca tggtcaaatt
     4261 catcgaggaa ggcgacaagg tcaaagtgac catgcgcttc cgcggtcgcg agctcgcgca
     4321 tcaggagatc ggcatgaatg tgctcatgcg cgtcaaaggc gatctcgacg aggtcgccaa
     4381 ggtcgagcag catccgcgca tggaaggccg ccagatgacc atggtgatgt cgccgaaata
     4441 gcagctcgag gcggcgggaa cgagctcgcc gattgtgaca tcgcgggcac tcgcccgcgc
     4501 tgctttttca catcattcgc gggccgtcat gctcccgatt gcgcgaacgg ttgccgtcac
     4561 gcgaacgtga ccaacctgcg cttgtcttcg tccgaaggtc cactttagaa cgattcgcgt
     4621 ccagaggcgc gtcgcggcaa aacgaacgcg cactgggcgg cccgttgggg atcctctgcg
     4681 gcacggactt caaatttacg ggagattgtc catgaagggt cacattctag cggctgccgc
     4741 tctggcagcc gggtctcttg ccatgtcggt ggcgccggcg aaagcacagg aattcactgc
     4801 cacattctcg gggttccagg agaccggcgc cctgaacaat gagtctggct cgattctctc
     4861 gaacggcacg gccaagctca ggctgaacgt caacaaggtc gctcagactg cgaccttcac
     4921 gctcaccttc agcgggctca gcacgccgat cacccagtcg catatccatt tcggccgggt
     4981 gcatacgccg ggcaacgtca tcgtcttctt ctgctccaat ctcaacaatg gcccggccgg
     5041 gacccagctc tgcccgcaga acgcgacgag cgggaccgtc accgggacga taacggcggc
     5101 ggatatcatt gcggtaccct cgcagggcgt ccccgccgat aatttcgacg cgctgacgga
     5161 tgcgctcaac tccaacaccg cctatggcaa cgtccacacg acgcagttcc cgaacggcga
     5221 gattcgcggc caagtcctcg tcggcgagct cggcgacttc gagaactaac acgaacgtcc
     5281 cttgacctgt cgcggctgtc ccttaccggg acagccgcgc gggtgtcagc ggcatctttg
     5341 gaaggaacgc tgcgctctcg cattggcctg cgccgcgctt gcttttccgg ggtcatccct
     5401 ttataaaccg cgccttatcg ggcggctggc tgcgccttcg ggcgctcggg gcatgcctcg
     5461 cgccccaagc catacccctc cgaaaggacc gaaatgccca agatgaagac caagagcggg
     5521 gcgaagaagc gcttcagccg caccgccacc ggcaagatca aggcgaatgt cgctcacaag
     5581 cgccaccgtc tgatcagcaa gcccaagaag atgaagcgca aggcgcgcgg caccttcgtc
     5641 ctcgagaagg gcgacgcgcg gctcgtgctc acgtatatgc cgtatttgcg gggctgagcc
     5701 atggcgcggg tcaaacgggg cgtcacgacg catgcccgtc acaagaaggt gctgggcctc
     5761 gccaagggct atcgcggccg ggcgaagaac gctttccgca tcgccatcga gaaggtcgag
     5821 aaggggctgc gctatgccta tcgcgaccgg cgcgccaaga agcggaactt ccgcgccttg
     5881 tggatccagc gcatcaatgc cggcgcgcgt gccgaagggt tgacctactc cgaattcatc
     5941 aacggcatga agcgcgcagg catcgagctc gaccgcaagg tgctggccga cctcgcggtg
     6001 cgcgagcccg aggccttcaa ggcgatcgtc gcccaggcgc aggcggcgct gtcccaagct
     6061 gcgccggcat gacgacgggc acgcctcgat ttagccgcgg gacttgtcgt taggccccgc
     6121 ggcagcgcgt gttcaaatcg cgagagaaag gggccggagc gccccttttt ttcgtgctga
     6181 tgagattgcc cgatggaaac gctcgatcat atccgcgacg agatccttgg cctgatcaag
     6241 gcggcgacga gccgcgacgc tctggagcag gcgcgcgtcg ccctcctcgg caagaagggc
     6301 cgggtgactg cgctgatgaa gggcctcgcc ggcctcgatc cggacgcacg gcgccagcaa
     6361 ggcgctgctc tgaaccgcct caaggacgag gtcgaggaag cgataggcca tgccgcgcgc
     6421 cgcctcgaga gcgaagccct gacgcagcgg ctcgccggcg agcgcctcga cgtgacgctg
     6481 cccgtcgcgg cagagtcgca gggccgcatc catccgttga gccagaccat cgacgaattg
     6541 gtggcgatct tcggcgagat gggcttcgtc gtcgccgagg gccccgacat cgaggacgat
     6601 ttccacaact tcaccgccct caacatcccg ccggagcatc cggcgcgcca ggagcaggac
     6661 acgttctatc tgaaggcggc gagcgagagc ggccgcaagg tgctgcgcac ccatacctcg
     6721 ccggtgcaag tccgcaccat gctcgcccag aagccgccga tccgcgtcat ctgccccggc
     6781 cgcaccttcc gcagcgacca tgacgcgacg cattcgccga tgttccacca ggtcgagggc
     6841 ctggtgatcg accagacgac ccatatgggg catttgcgcg gctgtctcgt cgagttctgt
     6901 cgcgccttct tcgatgtcga cgatctgccg ctgcgcttcc gtcccagcta cttccccttc
     6961 accgagccat cggcggaagt ggatatcggc tgctcgcgcg ccggcggcga gctcaagatc
     7021 ggcgccttcg gcgattggct cgagatcctc ggctgcggca tggtcaatcc gcaggtgctg
     7081 cgcaattgcg gcatcgaccc cgcgatctat cagggttttg ccttcggcgt cggcatcgag
     7141 cgcatcgcca tgctcaaata cggcatcccc gatctccgca ccttctatga gagcgacctg
     7201 cgctggctca ggcattacgg cttcctgccg ctcgacgtgc cctccatgtc gggaggactc
     7261 gcgcgatgaa gacgacgctc gcctggctca agacgcatct cgagaccacg gcatcgctcg
     7321 acgccatcgt cgagaagctg gtgatgctcg gcctcgaagt cgatgcggtc gagaaccgcg
     7381 gcaaggcact cgcgcctttc gtcgtcgcgc aggtgctttc ggccgagtcc catcccaacg
     7441 ccgacaagct ccgcgtctgc atggtcgaca ccggcagcga gcggctgcag gtggtctgtg
     7501 gcgcgcccaa tgcgcgcgcc gggatgcgcg gcgtcttcgc gcccgccggc accgtcatcc
     7561 cgcgcagcgg cgtggtgctg aaggagagca tgatccgcgg cgtcgcctcg aagggcatgc
     7621 tctgctccgg atacgagctc ggtctttccg aggaccatga ggggatcatc gaattggcag
     7681 cggatgcgcc ggtgggcgcc tccttcgcgc agctgatggg gctcgacgat gccgtgctcg
     7741 atatcaaggt gacgccgaac cgcggcgatt gcctgggcgt caggggcatc gcgcgcgatc
     7801 tcgccgcctc cggcatcggc cggttgctgc cgctcgacgc cacgcccgtg cccggccgct
     7861 tcccctctcc ggtcgccgtt catctggcgg gccccgacga caaggcgtgt ccgctcttca
     7921 tcggccggat gatccgcggc ctgcgcaacg ggccgagccc gcgttggctg caggaccggc
     7981 tgacggcgat tgggctccgg ccgatctcgg cgctggtcga catcaccaat tttctcactt
     8041 tcgacgcggg gcggccgctc catgtcttcg atgccgacaa gatcggcggc gatctcgtcg
     8101 tccgcggcgc ccgcccgggc gagactctgc cggcgctcaa cggcaagacc tatgcgctcg
     8161 atcccgagat ggcggtgatc gccgatgatg acgaagtcct gagtctcggc ggcgtgatcg
     8221 gcggcgcgag cagcggctgc agcgacgcga cgaccgacgt cttcatcgag gcggcgctgt
     8281 tcgatcccgt gcgcaccgcg gccaccgggc gcaagctcca gctgcagagc gacgcgcgct
     8341 accgcttcga gcgcggcgtc gatcccgaat tcacccgtcc cggcctcgag atcgcaacgc
     8401 ggatgatcct cgagctctgc ggcggcgagc caggcgaagt tacgaccgct ggggcagtgc
     8461 cgaagccgat ccccgctctg gcgttcccgc cggcgctcgt gcgcagcctc ggcggcgtcg
     8521 acgtgccgac cggggaatgc gtgggcatcc tcggagcgct cggcttcgtc agcgaggcgc
     8581 aagcgggcgc cttgctggtc cgcgtgccct cctggcggca cgacatcgcc ggcaatgccg
     8641 atctcgtcga ggagatcctg cgcgtccacg gctatgacca cattcccgcg gtgccgctgc
     8701 tgcgcgagac gccgctcccc aaaccggcat tgacgccggc gcagcgccgc accggcttcg
     8761 tccgccgctc gctcgccgcg cgaggcctgg tcgaggccgt gaccttctcc ttcatgcacg
     8821 ggaaaatcgc ggagctcttc ggccaggttc ccgacacgct gcgcctcgcc aatccgatca
     8881 gcgccgatct cgatgtcatg cgtccctcga tcctgccgaa tctgctgggg gcggcgcagc
     8941 gcaatgccga tcgcggcttt cccgacggcg cgctcttcga agtcggcccg caataccgcg
     9001 acgacacgcc cgaaggccag gatctcatgg cggccggcat gcgcagcggt cgcaccggcc
     9061 ccaagcgctg ggacgatccc ggccatcccg tcgacgccat gatggcgaag gccgacgcgc
     9121 tggctgccgt ggccgcttcc ggccttgcgc ccgagagcgt ccaggtgagc gccgacccgc
     9181 ccgactggta ccatcccggc cgcgccggca cgctgcggct cggccccaag gtgctcggat
     9241 atttcggcga gcttcatccg ggggtgctcg aggagatgga cgtgacgggc cccgccgtcg
     9301 gcttcgagat ctttctcggc catgtgccgc tgccgcgcgc gaaacagggt cgctcccgcc
     9361 cgccgctgaa actgtcgccg cttcagccgg tcgagcgcga tttcgccttc gtggtgccgg
     9421 ccgagctgcc cgcggagacg ctgctgcgcg ccgcgcgcac tgtcgacaag cgtctcgtca
     9481 gggatgtgcg gctcttcgac gtctatcacg gtccgggcgt cggcgagggc aggaaatcgc
     9541 tcgccatcac cgtggtcctg cagcccgaag aggcgacgct caccgacaag gagatcgagg
     9601 ccttctcgga aaaactcatc gcgcaggtcg agaaggcgac cggcggcagc ttgcggcgct
     9661 gacaggaatg gtggcgttcg cgaagatccg caaagcggcg gagaaacgtc tcggcgccga
     9721 cggggtggcg gcgcggctgc cgccggtcaa atcgcccgcg cagctcaaga agctgggcga
     9781 tgaccgctat ctctcgctca tgtcgctgcg catctttcgc gccgggctca agcacgagat
     9841 ggtcgatgcc aaatggccgg ctttcgagac ggcgttccag ggcttcaacc cgaagcgcgt
     9901 gcgcgccatg agcgacgagc aggtcgaggc gctgatgaag gatgcgcagc tcatccgtca
     9961 tctaggcaag ctcaaagccg tgcatgccaa cgccgcgacg atgtgcacgc tggccgagga
    10021 gcatggcagt ttcgccgcct ggctcgcggc ttggcccgcg agcgagatca ccaagctctg
    10081 ggacgccttg gcgaagcgtt tcgcgcagct cggcggc
//
LOCUS       DASYNS010000385         5243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3997690, whole genome shotgun sequence.
ACCESSION   DASYNS010000385 DASYNS010000000
VERSION     DASYNS010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3997690"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="VGP50_12050"
     CDS             complement(<1..1484)
                     /locus_tag="VGP50_12050"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HEV8028156.1"
                     /translation="MCAAPADGSDAGRIDDALPAQTYVGQPLRRREDFRLLTGKGRYV
                     DDIKALGMLHMALLRSPHAHARVARVDLAAARAAPRIRLALAGADLVGKIGGIRPNWV
                     IPGTRVPERPVLAIDRVRFVGECVALVVAETREAALDALELIEVDYDTLPAVVDEALA
                     IGDDAPLLHENVPNNIATYYKIGGGDYAEAARRADQVLRLRIVNNRLIPTCLETRAIL
                     AEPGADGLLTVYMPSQVPHMHRRWIAETVGISEHRLRVVAPDIGGGFGAKMHLYPEEL
                     LCAYLALALGAPVKWWESRSESHQATSHGRAHTEQIEVAFRNDGRILGMRVETLGNVG
                     AYLSNMASGGPTMNTVNFGTGNYKIENYEAVAKVIVTNTVPVDAYRGYGRPEGAYIAE
                     RTIEAVARHLDLDPVAVRRINFVPRSAFPYRPYGSRAVIYDSGDYEGCLEKALAAFDY
                     AARREEQRALRGSGRYRGIGVAAYTEMCGMAPSRRLAASGFDRGG"
     gene            complement(1487..2680)
                     /locus_tag="VGP50_12055"
     CDS             complement(1487..2680)
                     /locus_tag="VGP50_12055"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV8028157.1"
                     /translation="MGGPLSGIRVVDFSRVLAGPLCARTLLELGAEVIKIEPPNPDVA
                     RFAFPAKDGMSGYYAQQNAGKLNVSIDLNVPGSLDLALRLCDTADVVVENFRAGTLGF
                     FGLDYATLAGQGGPWRGRMAYAPTVQAEAGFTENSVRHYGDALRELRTDSLSHADVYS
                     GLQAVIAILAALHRRKATGTGQYIDVAMAATLMAVNERAHVDLSEEDLGAEPAILGAT
                     DCPFFRGPNGEEFTVATSIVGSRTFPSYMHAMRRGDLIDDPRFATAAARRRNFAALHA
                     IIQAWILTFRDVASLDAQLDEAKIAMGEVRSIRDLSRSDWSTYWGAVQEVSDRHGGVY
                     CVPGRPWRFSRDTLEPAGAPAFQGEHNRSVFTQLGLTADEIDRHVTSGALVSRVPVGA
                     TQGSS"
     gene            complement(2682..3473)
                     /locus_tag="VGP50_12060"
     CDS             complement(2682..3473)
                     /locus_tag="VGP50_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV8028158.1"
                     /translation="MTVAEEPLVTSEQRGAVAILTMAYRPHNLLGPKLTGAIAREVKL
                     AVETGSRAIVLRSGLRHFCAGADVGLFAERINNAGRSEAKLSGIEFLRLLELLPVPIV
                     ASVHGVCLGGGLELALACDYIIAASSSKLGSVEAALGLHPLMGGIQRQVQRIGAARAK
                     EMSMLARRYDAATLERWGLINLVVADESLEQATLSVAQELAFGPTIAHAATKQLAYLA
                     ANEGVLAADLAMTKIQEPIWASEDLKTGLASFRQNGPGLAKFQGR"
     gene            complement(3605..4198)
                     /locus_tag="VGP50_12065"
     CDS             complement(3605..4198)
                     /locus_tag="VGP50_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8028159.1"
                     /translation="MRYDAGHKQRTRTKVLKEAARAIRSDGPHRVGVAGIMAKAGLTH
                     GGFYAHFDSKDDLVAATMAQMFDEALATFDRLTLGKAPAVALRAYVDFYLSPRHRDAH
                     DTGCPLPALSADLPRLDQAARTGFAQGIERLTAALAGLIAAFGHDAAADVASSALAEM
                     VGAISLARAIPDAKRSNAVLKRSRDALTHRLRIIEKD"
     gene            complement(4299..>5243)
                     /locus_tag="VGP50_12070"
     CDS             complement(4299..>5243)
                     /locus_tag="VGP50_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEV8028160.1"
                     /translation="LMIEATAVEPEGRISLGDLGLYGDENEAALHHVLRACRDFGNTP
                     IGIQLAHAGRKASAQVPWKGGGPLKPEEGAWPTPAPSAVPFVEGWPAPVALDRGGLER
                     IRHAFAAAARRAERLGIDVVEMHAAHGYLLHQFLSPLANQRSDAYGGSRTNRQRFPLE
                     VAQDLRRAWPRALGARISGSDWMAGGVEIEDAIAFAGELKALGFDYVCVSSGGIVPNA
                     PIKIGPGYQVPLAAQVRAAVGIATRAVGLIVEAKQAERIVESGAADLVALARSFIDDP
                     RWVWHAAETLGAKVTYPPQYRRAEPKLWPGAALAHELD"
BASE COUNT          892 a         1762 c         1720 g          869 t
ORIGIN      
        1 ccaccgcgat caaacccgct ggccgccaga cggcgcgacg gcgccatgcc gcacatctcg
       61 gtataggcgg cgacgccgat gccgcgatac cggccgctgc cgcgcagcgc gcgctgctcc
      121 tcgcgccgcg ccgcatagtc gaaggccgcg agcgccttct ccaggcagcc ttcgtaatcg
      181 ccgctgtcgt atatgacggc cctgctgcca tacgggcgat agggaaacgc cgagcgcggc
      241 acgaaattga tgcgccgcac cgcgacggga tcgagatcga gatgccgcgc caccgcctcg
      301 atcgttcgct ccgcgatata agcgccttcc ggccgcccat atccgcgata ggcgtcgacg
      361 ggcacggtgt tcgtcacgat caccttcgcg accgcttcgt agttctcgat cttgtagttg
      421 ccggtcccga agttgacggt gttcatggtc gggccgcccg aagccatgtt cgagagatag
      481 gcgccgacat tgccgagcgt ctcgaccctc ataccgagga tgcggccgtc gttccggaag
      541 gcgacctcga tctgctcggt atgggcccgg ccatggctcg tggcctgatg gctttcggag
      601 cgggactccc accatttgac cggcgcgccc agcgccaacg ccagataggc gcacagcagc
      661 tcttcgggat agagatgcat ctttgccccg aagccgccgc cgatatccgg cgccaccacg
      721 cgcaggcggt gctcagagat gccgacggtc tcggcgatcc agcggcgatg catatgggga
      781 acctggctcg gcatatagac ggtgaggagc ccgtcagcgc ccggctcggc gaggatcgcg
      841 cgcgtctcga gacaggtcgg gatgaggcgg ttgttgacga tgcggaggcg caggacctgg
      901 tccgcccggc gagccgcttc ggcataatcg ccaccaccga tcttgtaata ggttgcgata
      961 ttattcggaa cattctcgtg gagcagcggc gcatcgtcgc cgatcgccag cgcctcgtcg
     1021 accacggcag gcaaggtgtc gtagtcgacc tcgatcagct ccagcgcatc aagggcggcc
     1081 tcgcgcgtct ccgcgacgac gagcgccacg cattcgccga cgaagcgaac ccggtcgatc
     1141 gcgagcactg gccgctccgg gacccttgtc cccgggatca cccagttggg ccggatgcca
     1201 ccgatcttgc cgacgagatc ggcgccggcc aacgccaagc ggatgcgcgg cgccgcgcgc
     1261 gctgccgcga ggtcgacgcg cgcgacgcgg gcatgggcgt gcggcgaacg gagcaacgcc
     1321 atgtgcagca tccccagcgc tttgatgtcg tcgacgtagc gacccttgcc ggtaagcagc
     1381 cggaaatcct cccgccggcg gagcggctgg ccgacatagg tctgcgccgg caaggcgtcg
     1441 tcgatccgcc ctgcgtccga gccatcggct ggcgcggcac acatgatcag gaggaaccct
     1501 gcgtggcccc cacggggaca cgcgatacca aggcacccga cgtgacgtgc cggtcgatct
     1561 cgtctgccgt caagcccaat tgggtgaaga cgctgcggtt gtgttcgccc tggaaagccg
     1621 gcgcgcccgc cggctcgagc gtgtcgcggg aaaagcgcca cgggcgcccg ggcacgcaat
     1681 agacgccgcc atggcgatcc gatacctcct ggacggctcc ccaatacgtg ctccagtccg
     1741 atcgcgagag gtcgcgaatg gaccgcactt cgcccatggc gatctttgcc tcgtcgagct
     1801 gggcatcgag acttgcgacg tcgcggaagg tcaggatcca ggcctgaatg atcgcgtgca
     1861 gcgccgcaaa attccggcgt cgcgcggcgg ccgtcgcaaa acgcggatcg tcgatcagat
     1921 cgccgcggcg catcgcgtgc atgtaggaag ggaaggtgcg actgccgacg atgctggtgg
     1981 caacagtgaa ttcttcgcca ttcggtccgc ggaagaacgg gcaatcggtt gcaccgagaa
     2041 tggccggctc tgcgcccaga tcctcttcgg agagatcgac atgggcgcgt tcattgaccg
     2101 ccatcagcgt cgccgccatc gccacgtcga tatattgccc ggtgccggtc gccttccgcc
     2161 ggtggagcgc ggcgaggatc gcgatcacgg cctgaaggcc cgaatagacg tcggcatgcg
     2221 agaggctgtc cgtccggagc tctctcagcg catcgccata gtggcgaaca ctgttctcgg
     2281 taaatcctgc ctcggcctgc actgtcggcg cataggccat gcgtccccgc caggggccgc
     2341 cctgcccggc gagcgttgca tagtccagcc cgaagaagcc tagcgttccg gcgcgaaagt
     2401 tctcgacgac gacgtccgcc gtatcgcaca ggcggagcgc cagatcgagc gagcccggga
     2461 cgttgagatc gatgctcaca ttgagctttc cagcgttctg ctgggcgtaa taacccgaca
     2521 tgccgtcctt cgcgggaaac gcgaacctgg ccacgtcggg attgggtggc tcgatcttga
     2581 tcacctctgc gcctagctcg aggagcgtcc gcgcgcagag cggcccggcg agaacacggg
     2641 aaaaatcgac gacgcgaatg ccgctcaagg gcccacccat gtcagcgccc ctggaatttg
     2701 gcgagacccg gtccgttctg cctgaacgat gcgaggccgg ttttaagatc ctccgaggcc
     2761 cagatcggtt cttgaatctt cgtcatcgcc aggtcggccg ccagcacgcc ctcattggcc
     2821 gccagatatg ccagctgctt ggttgccgcg tgggcgattg tgggcccgaa agcgagctcc
     2881 tgcgcgaccg acagcgtcgc ttgctcgaga gattcatcgg cgacgacgag gttgatcagg
     2941 ccccagcgct cgagcgtcgc ggcgtcgtag cgccgcgcga gcatcgacat ctccttcgcc
     3001 cgagcggcgc cgatgcgctg gacctgtcgc tggattccac ccatcagcgg atgcagtcca
     3061 agtgccgctt ccaccgatcc aagtttcgat gaggacgccg caatgatgta gtcgcaggcg
     3121 agtgccagct ccaggccgcc gcccaggcac acgccgtgga cgctggcgac gatgggaacg
     3181 gggaggagct cgagcagccg caggaactcg atcccgctca gctttgcctc ggagcggccg
     3241 gcgttgttga tgcgctcggc aaagagaccg acatcggcgc cggcgcagaa atggcgcaag
     3301 ccgctccgca gcacgatggc gcgactgccg gtctccacgg caagcttcac ttcccgcgcg
     3361 attgcaccgg tcagcttggg cccaagcagg ttgtgcggcc gataggccat cgtaaggatt
     3421 gccaccgccc cgcgctgctc gctcgtaacg aggggctctt ccgcgactgt catttttctc
     3481 gtctccgtct cgactcaacg gcctgctggc taccatgata tgacactcat catataaact
     3541 ctgctctcgt ctccgcgctc atgtcaagag ccttgccgcc gccggccgcg ctcaggacag
     3601 agcgtcagtc tttctcgatg atgcgaagac gatgcgtcag cgcgtcgcga gaacgcttga
     3661 gtaccgcatt cgaccgtttg gcgtcgggga tggcgcgcgc caaggatatg gcgccgacca
     3721 tctcggcaag cgccgagctt gcgacgtccg cggcagcgtc gtgcccgaag gcggcgatca
     3781 gacccgcaag cgccgccgtc agccgctcga taccttgcgc aaagccggtt cgagccgctt
     3841 gatcaaggcg cgggagatcg gccgaaagcg cgggcagcgg gcacccggtg tcatgggcat
     3901 cgcggtgtct cggggacaga tagaagtcga cataggcgcg caacgctacc gccggcgctt
     3961 tcccgagggt caaacgatcg aaggtcgcca gcgcttcgtc gaacatctgc gccatcgtgg
     4021 ccgccacgag atcgtccttc gagtcgaaat gggcgtagaa tccgccatgg gtcaacccgg
     4081 ccttggccat gatgccggcg acgcccaccc gatgaggtcc gtcgctgcgg atcgcccggg
     4141 cggcctcttt gagaaccttg gtccgggtcc tttgtttgtg gccggcatca tagcgcatga
     4201 acgttctccg ctccttcgta tgacagatat aatactatcc tatcattccg gcagcgctgg
     4261 caagcgttct tgccgtgccc gcgccgtggc gcagcctctc aatcgagctc atgggcgagc
     4321 gccgcgcccg gccagagctt tggttcggcg cgccgatatt gcggcggata ggtgaccttg
     4381 gcacctaacg tctcggcggc gtgccagacc cagcgcggat cgtcgatgaa gctccgggcg
     4441 agggcgacga gatcggccgc gccgctttcg acgatgcgct ccgcctgctt tgcctcgacg
     4501 atcaggccga cggcgcgcgt cgcgatgccg acggcggcgc gcacctgcgc ggccaagggg
     4561 acctggtagc cggggccgat cttgatcggt gcgtttggaa cgatcccgcc actcgataca
     4621 cagacatagt cgaagccgag cgctttcaat tcgccggcaa aggcgatcgc gtcctcgatc
     4681 tcgactccgc cggccatcca gtcgctgccg ctgatgcggg caccgagcgc gcgcggccag
     4741 gcgcggcgca gatcctgcgc cacctcgagc ggaaagcgct gccgattggt gcggctgccg
     4801 ccatacgcgt cgctgcgctg attggccagc ggcgacagaa attgatgcag cagatagcca
     4861 tgcgccgcat gcatctcgac gacgtcgatg ccgagccgct cggcgcgccg cgccgcggcg
     4921 gcaaaggcgt ggcggatgcg ctcgaggcca ccgcggtcga gcgccaccgg cgccggccag
     4981 ccctcgacga aggggaccgc ggacggcgcc ggcgtcggcc aagcgccctc ttcgggcttg
     5041 agcggcccgc cgcctttcca gggcacttgc gccgaggctt tgcggccggc atgcgcgagt
     5101 tgaatgccga tcggcgtgtt gccgaagtcg cggcaggcgc gcagcacatg atgaagcgcc
     5161 gcctcgttct catcgccata gaggccgaga tcgccgaggc tgatgcggcc ttcgggctcg
     5221 accgccgtcg cctcgatcat gag
//
LOCUS       DASYNS010000386         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2281890, whole genome shotgun sequence.
ACCESSION   DASYNS010000386 DASYNS010000000
VERSION     DASYNS010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281890"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VGP50_12075"
     CDS             <1..883
                     /locus_tag="VGP50_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV8028161.1"
                     /translation="GAGKEVLARVLHAHSRRARGPFVAVNCATMRPERLEIELFGIEA
                     GADGPDSPRKVGTFEQAHGGTLFLDEVADMPLETQGKIVRVLQEQVFERVGGNRVEVD
                     VRVIASTNRDLTHEIAMDRFREDLYYRLAVVPLRVPALRERREDIALFARHFMRRSAE
                     AARLAPREFGEDALAALRAYEWPGNVRQLRNVVDWLLIMAPGEPREPIRAEMLPAEIG
                     SIAPNVVKWDKGGEIMTLPLRDAREVFEREYLLAQVTRFGGNISRTAAFIGMERSALH
                     RKLKSLGVGNSAAKLGA"
     gene            1049..2128
                     /locus_tag="VGP50_12080"
     CDS             1049..2128
                     /locus_tag="VGP50_12080"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEV8028162.1"
                     /translation="MTGVVAATAAYEAWMRRRIAVVGRDLKHKHECMAQSAFEFMRAT
                     FYRFVPLWQEACGELCDAPKLLAVGDLHVENFGTWRDAEGRLVWGVNDFDEAYAMPYA
                     IDLVRLAVSALLAIREDRLRIAGGEACDALLGGYREMIEADGGSGFVLEEKHPALRTM
                     AMGAERDAERFWAKLAANKRVNAPAAVRRMLRHALPEVDGEVEILHRTAGLGSLGRPR
                     YVAIGRARGGMVAREAKAMLPSAFAWANGIAEERFFCGKLAARAIRAPDPFYRTEKAW
                     VLRRLGPHCSAILLADYPKRRDERCLLEAMGRETANIHWGSPEKIAAIRRDLKRRKGP
                     WLRDAAATMAEATLRDWKAWRKARA"
BASE COUNT          360 a          793 c          843 g          364 t
ORIGIN      
        1 cggcgccggc aaggaggtgt tggcgcgggt gctgcatgcc cactcgcggc gcgcgcgggg
       61 gcccttcgtc gcggtcaatt gcgcgacgat gcggcccgag cgcctcgaga tcgagctctt
      121 cggcatcgag gcgggcgccg acgggcccga cagcccgcgc aaggtcggca ccttcgagca
      181 ggcgcatggc ggcaccttgt tcctcgacga agtcgccgac atgccgctcg agacgcaagg
      241 caagatcgtg cgcgtgctgc aggagcaggt gttcgagcgc gtcggcggca accgggtcga
      301 ggtcgacgtc cgggtcatcg cctcgaccaa tcgcgatctc acccatgaga tcgccatgga
      361 ccgctttcgc gaggatcttt actaccgcct cgccgtggtg ccgctgcgcg tgccggcgct
      421 gcgcgagcgg cgcgaggaca tcgcgctctt cgcccggcat ttcatgcgcc gctcggccga
      481 agcggcgcgg ctggcgccgc gcgagttcgg cgaggacgcg ctcgcggcgc tgcgcgccta
      541 tgaatggccg ggcaatgtgc gccagctgcg caatgtcgtc gactggctcc tcatcatggc
      601 gccgggcgag cccagagagc cgatccgcgc cgagatgctg ccggccgaga tcggctcgat
      661 cgcgcccaac gtcgtgaaat gggacaaggg cggcgagatc atgacgctgc cgctgcgcga
      721 cgcgcgcgaa gtgttcgagc gcgaatatct gctggcgcag gtaacgcgct tcggcggcaa
      781 catctcgcgc accgccgcct tcatcggcat ggagcgctcg gcgctccacc gcaagctcaa
      841 atccctgggc gtcggcaaca gcgcggcgaa gctcggcgct tgagttgcgc gtccggcccc
      901 ctcaccctcc cgccgcttcg cggcgggtcc ctccctctcc ccgaggggag aggggaaagg
      961 acgccgccac cgcgtcccct ctccccatcg gggagaggga cagggtgagg gggccgtcga
     1021 tcgcagagcc actgattggc gcgaggccat gaccggcgtc gtcgcggcca ccgccgccta
     1081 tgaagcctgg atgcggcggc gcatcgccgt ggtcgggcgc gatctcaagc acaagcatga
     1141 gtgcatggcg cagtccgcct tcgagttcat gcgcgccact ttctatcgct tcgtgccgct
     1201 gtggcaggag gcgtgcggcg agctctgcga tgcgccgaag ctgctcgccg tcggcgatct
     1261 ccatgtcgag aatttcggta cctggcgcga tgccgagggg aggctggttt ggggcgtcaa
     1321 tgatttcgac gaagcttatg cgatgcccta tgccatcgac ctggtgcggc tcgctgtgag
     1381 cgctctgctg gcgatccgcg aggaccggct ccgcatcgcc ggcggcgagg cctgcgacgc
     1441 gctccttggc ggctaccgcg agatgatcga ggccgatggc ggcagcggct tcgtcctcga
     1501 ggagaagcat ccggcgctcc ggacgatggc gatgggcgcg gagcgcgatg ccgagcgctt
     1561 ctgggcgaag ctcgccgcca acaagcgggt gaacgcgccg gcggcggtgc ggcggatgct
     1621 gcgccacgcg ctccccgagg tggacggaga ggtcgagatc ctgcatcgca ccgccggcct
     1681 cggcagcctg gggcgaccgc gctacgtcgc gatcggccgt gccaggggcg gcatggtcgc
     1741 gcgcgaggcc aaggcgatgc tgccttcggc cttcgcctgg gccaacggca tcgcggagga
     1801 gcgcttcttc tgcggcaagc tggcggcgcg cgccatccgc gcgcccgatc ccttctaccg
     1861 cacggagaag gcctgggtgc tgcgccgcct cgggccccat tgcagcgcga tcctcctcgc
     1921 cgactacccg aagcgccgcg acgagcgctg ccttctcgag gccatgggcc gcgagaccgc
     1981 caatatccat tggggctcgc ccgagaagat cgccgcgatc cgccgcgacc taaagcgccg
     2041 caagggcccc tggctgcgcg acgccgcggc gacgatggcg gaggccacgc tcagggattg
     2101 gaaggcgtgg cgcaaagcgc gggcgtaagg cagagtgctg caaaacggct tctgcgtcct
     2161 tcgacaagct caggatgagg atgatcggag tcgggcatct gctagatgcc agtcccgctg
     2221 ctcctcatcc tgagcttgtc gaaggacgca catcggaatt gcagcgcgta aagggaggat
     2281 gacgtgctga cggccgctga acgcaagcac caggtcctgt cggtgatccg cgtctccagc
     2341 ggcaatttcc tggagatgta
//
LOCUS       DASYNS010000387         1833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1968653, whole genome shotgun sequence.
ACCESSION   DASYNS010000387 DASYNS010000000
VERSION     DASYNS010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1968653"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VGP50_12085"
     CDS             <1..232
                     /locus_tag="VGP50_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028163.1"
                     /translation="STLHQPASVFRMGGPTIDASMLWPLLIMALGFTLAFVALLLIRM
                     RTALIAAKIRALRLAEVERQTRGMVVQQTAWS"
     gene            229..408
                     /gene="ccmD"
                     /locus_tag="VGP50_12090"
     CDS             229..408
                     /gene="ccmD"
                     /locus_tag="VGP50_12090"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEV8028164.1"
                     /translation="MSTLATFLAMGGYARFVWPAYGLAAAVLLGMTVWTLGSYRRRQK
                     ELAQLQREAPEPRRR"
     gene            405..860
                     /gene="ccmE"
                     /locus_tag="VGP50_12095"
     CDS             405..860
                     /gene="ccmE"
                     /locus_tag="VGP50_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEV8028165.1"
                     /translation="MTRKRRRLIGVLAGLGMLGIATALVLTAFSDNLVFFYSPSDLKT
                     KHVAEGRRLRIGGLVENASLKREADGKTFEFSITDGENSVPVTYTGLLPDLFREGQGV
                     VIQGMIGSDGMVRADEVLAKHDERYMPKEVVDALKKSGHWQEGEGTVAR"
     gene            857..>1833
                     /gene="ccsA"
                     /locus_tag="VGP50_12100"
     CDS             857..>1833
                     /gene="ccsA"
                     /locus_tag="VGP50_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEV8028166.1"
                     /translation="MIAELGHFALILALLTALVQSSVPLIGAARRQLSWMAVAKSTAL
                     MQFVLVAIAFAALMYAYVTSDFSVANVYENSHSAKPLLYKITGVWGNHEGSMILWVFM
                     LSLFGAAVAWFGNNLPPALRARVLAVQGMIGLGFLLFILLTSDPFTRLSPAPLDGRGL
                     NPVLQDPGLAFHPPFLYLGYVGFSVAFAFAVAALIEGRVDAAWARWVRPWTLAAWCAL
                     TIGIAMGSWWAYYTLGWGGWWFWDPVENASFMPWLVGTALLHSAIVVEKRDALKSWTI
                     LLAIVTFSLSLIGTFLVRSGVLTSVHAFAVDPRRGLFILLLLFIAIGGSL"
BASE COUNT          248 a          627 c          609 g          349 t
ORIGIN      
        1 gagcacgctg caccagccag cgagcgtctt ccgcatgggc gggccgacca tcgacgcctc
       61 gatgctgtgg ccgctcctga tcatggcgct gggcttcacc ttggccttcg tcgctctctt
      121 gctcatccgc atgcgcacgg ctctcatcgc cgccaagatc cgcgcgctgc gcctcgccga
      181 ggtggagcgc cagacgcgcg gcatggtggt ccagcagacg gcgtggtcgt gagcacgctg
      241 gcgaccttcc tggcgatggg cggctacgcg cgcttcgtgt ggccggccta tgggttggcc
      301 gcggccgtgt tgctgggcat gaccgtctgg acgctcggca gctatcgccg ccgccagaag
      361 gagctcgcgc agctgcagcg cgaggcgccc gagccgcgcc ggcgatgacg cggaaacgcc
      421 gccgcctcat cggcgtcctc gccggcctcg gcatgctcgg gattgccacg gccctggtgc
      481 tgacggcgtt cagcgacaat ctcgtgttct tctacagccc gagcgacctc aagacgaagc
      541 atgtggcgga aggacggcgg ctccggatcg gcggcctcgt cgagaatgcg agcctgaagc
      601 gcgaggccga cggcaagacc ttcgaattca gcatcaccga tggcgagaac tccgttcccg
      661 tgacctatac gggcctcttg cccgacctct tccgcgaagg tcagggcgtg gtgatccaag
      721 gcatgatcgg aagcgacggc atggtgcgcg ccgacgaagt cctcgccaag catgacgagc
      781 gctacatgcc gaaggaagtc gtcgacgcct tgaagaagag cgggcactgg caggaaggcg
      841 aagggacggt ggcacgatga tcgccgagct tgggcatttc gccttgatcc tggcgctgct
      901 gacggcgctc gtgcaatcga gcgtgccgct catcggcgcg gcgcggcggc agctcagctg
      961 gatggccgtt gcgaagagca cggcgctgat gcagttcgtg ctggtggcga tcgcctttgc
     1021 cgcgttgatg tacgcctacg tcacctcgga cttctcggtg gccaacgtct acgagaacag
     1081 ccattcggcg aagccgctgc tctacaagat cacgggcgtc tgggggaacc acgaaggatc
     1141 gatgatcctc tgggtgttca tgctctcgct cttcggcgcg gccgtcgcgt ggttcggcaa
     1201 taacctgccg cccgcgctcc gcgcgcgcgt gctggcggtg cagggcatga tcggcctcgg
     1261 cttcctgctg ttcatcctgc tgacctcgga cccgttcacg cggctctcgc cggcgccgct
     1321 cgacggccgc ggcctcaatc ccgtcctcca ggatccgggg ctcgccttcc atccgccctt
     1381 cctttatctc ggctatgtcg gcttctccgt cgctttcgcc ttcgccgtgg cggccctcat
     1441 cgaggggcgc gtcgatgccg cctgggcgcg ctgggtgcgg ccctggacgc tcgccgcctg
     1501 gtgcgcgctc accatcggca tcgccatggg aagctggtgg gcctattaca cgctgggctg
     1561 gggcggctgg tggttctggg acccggtcga gaacgcgtcc ttcatgccct ggctcgtcgg
     1621 cacggcgctc ctccactccg ccatcgtcgt cgagaagcgc gacgcgctca agagctggac
     1681 gatcctgctg gccatcgtca ccttctcgct gtcgctcatc ggcacgttcc tggtgcgctc
     1741 gggcgtcttg acctcggtgc atgccttcgc cgtcgatccc cggcgcggcc ttttcatcct
     1801 gctgctgctg ttcatcgcca tcggcggctc gct
//
LOCUS       DASYNS010000388        10652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_876869, whole genome shotgun sequence.
ACCESSION   DASYNS010000388 DASYNS010000000
VERSION     DASYNS010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10652
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876869"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1708
                     /locus_tag="VGP50_12105"
     CDS             341..1708
                     /locus_tag="VGP50_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEV8028167.1"
                     /translation="MAIESINPATGDVVARYEEMAPAAVADIVAKTHEAFLGWRRTSF
                     TTRAQAMRKAAEILRSDASHYGKLMAQEMGKPLRDGIAETQKCATGCDFYAEHAARFL
                     APEPVATEARKCFVTFQPLGVVLAVMPWNFPFWQVFRFAAPGLMAGNAAVLKHASNVP
                     GCALAIEDVFRRAGFPENLFRTLMIGSRQVEGIIEHPLIRAATLTGSGPAGRAVASKA
                     GAMLKKTVLELGGSDPYLVLEDADIDQAAALSAKGRLINAGQSCIAAKRFIVLDKVRA
                     AFEERFVERMRAARMGDPLQEGTEVGPLARHDLRDALHQQVEASVAKGARCLLGGRIP
                     KDRGAYYPPTVLTDVRKGMPAYDEEMFGPVASVIAVKSEEEAIAVANDSPFGLGGGIF
                     SRDLARAERIAAEEIESGAVFVNETVRSDPRWPFGGVKESGYGRELSAYGIKEFVNIK
                     SVYIA"
     gene            1864..3231
                     /locus_tag="VGP50_12110"
     CDS             1864..3231
                     /locus_tag="VGP50_12110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV8028168.1"
                     /translation="MSSDYDVIVIGGGSPGEHCAGALAAGGLRVALVERELIGGECSY
                     WACIPSKTLLRPGEAAHGANEAAATAQVDVSATLAWRDFMVSNYSDSGQQRWLAEHGI
                     ALLRGRGRLAGTGVVEVDGARHTADHVVVATGAEPIIPPVPGLRGLEGIWTNREATGM
                     KAVPRRLLVLGGGPVGVEMAQAVRRLGGAVVLVEGAAYVLPHEPRPLGEALADVLRRD
                     GIELALGARATAARRESEHYALDLDDGRTLSGDRLLVATGRRPRVDGLGLETVGVAAN
                     KRGIPVDAQLRAGERLWAIGDVTGIWPLTHVGKYQGRVVAANILGEKREANYEAVPRV
                     TYTDPQAAAVGAAEASYSATARLAEVAKTATYTRAYAQSNGFLTLLSDGARLTGAYAL
                     GPEAGEWLQQATLAIRARVPLAVLRDTIQPFPTFSEIYLDALKALDGQIRTARPAAGA
                     ATKAR"
     gene            complement(3243..3881)
                     /locus_tag="VGP50_12115"
     CDS             complement(3243..3881)
                     /locus_tag="VGP50_12115"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8028169.1"
                     /translation="MNLYDTRLDFARGLIRHWLDIRGGALVPLEAEIDPNELAPIFDS
                     LGMIDLGLPYHMAVEFAGERLRKRFGRDLQQVNWLDLVPPAVRDAGKIARERARRMPC
                     AYYHSFTVSRADAPAVTVETLALPLRRRFAAVPGAVIVVTQDDSAETGWLTASARLAH
                     FSCKFVDLEYPFVRLGWRSCAVHPLLQNSRPSESRKVDRDGNLQIVGTAKTT"
     gene            complement(4333..4914)
                     /locus_tag="VGP50_12120"
     CDS             complement(4333..4914)
                     /locus_tag="VGP50_12120"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEV8028170.1"
                     /translation="MRPASLSRRLLIAGLAFAALTSGCTSPPSRRFLLAPRGAGAATA
                     PSFAGRVAIAGIGIAKYLDQPEIVRHHSGYELAVAEFEVWAEGMADMVGRVLAEDLAA
                     RLPGGTVFVGDSAATVPADTSLELYIDRFDADPDGMVVLNSRWTIRRGSGAPRLGAER
                     ITARPASPGTPDLVAAMSDALAALADRLAAQLA"
     gene            complement(4922..6568)
                     /locus_tag="VGP50_12125"
     CDS             complement(4922..6568)
                     /locus_tag="VGP50_12125"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEV8028171.1"
                     /translation="MKLLPPGLKRRPHVSPIWAVPAVAALVALWLVYTTLAAQGPRIT
                     ITFATADGLEAGKTQIKHNNVQLGVVETVELSQDLSHVVVRARMLKTASRHLNEGTRF
                     WVVRPRLSLTNFSGLETLVSGAHIEMDPGAGAYAEAFKGLELPPVVRADVPGREFVLM
                     TPKLGALGPGSPIYFRGIQVGEVLGYDFAAAEQDIAVHAFVRAPYDTLVREGTRFWSA
                     SGISLTPGGQGFKFQIESLQAVISGGIAFETPDTAQGAEPAKEGTVFALLDDHESVAE
                     SAYTVHARYRVEFEGSVHGLDPGAPVEFKGVKVGRVVDVHLEFDPRSNIVRVPVTLEL
                     ERQHLQQSADTPLPPGASLTAVLIEHGMRAQLRSSSLITGQLYVALDIFPRAPPDKLD
                     TSGPDPRIPTVPGDLEGITTSLTTVLDKISALPLDQLVRDLDATLQSYQRVADMPELR
                     QSMHSLSQALAAAESLMRNADTQAGPLLASLRKTSDAAQDAIAHADETLASVNSGYGD
                     DSQVRRDLTALLRQFQDAARSVKALADSLEQHPEALVRGR"
     gene            complement(6565..7803)
                     /locus_tag="VGP50_12130"
     CDS             complement(6565..7803)
                     /locus_tag="VGP50_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HEV8028172.1"
                     /translation="MTVAPVDDAGHSSMIVECRHCGLRHAAAETPAGHAALCRRCGAL
                     LLPRAAASREVSMALSLTGLILAAIANLMPLMSIRIEGRVEVASLASGALALAADGLW
                     PLAALIMLTTIVVPLARLGGTAYVLLQLSRPAPPHARTLFRWLERWRPWAMIEVYLLG
                     VFVAYVKLGDIAAIEIGAALHALVALMLVMATLDVTLDAEAIWKALAPGVESGGAGKL
                     ARCDACALVSVAAGGHPRCPRCGAALHARKEESLARCWALVIAALIFYLPANYLPVMT
                     VISFGNGAPDTILSGVEHLIVSGMWPLALLVFFASITVPVLKLLSLLLLLVTTQRGSR
                     WRIHERTRLHRIVEGIGRWSMIDIFMLSVLVALVRLGAIASVEPGAGALAFAAVVVVT
                     MLAATAFDPRLMWDSAGENP"
     gene            8187..8726
                     /locus_tag="VGP50_12135"
     CDS             8187..8726
                     /locus_tag="VGP50_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8028173.1"
                     /translation="MKGKRRSFAADACPIARTVDTIGDWWSLLIIRDAALGGKRRFGE
                     FQKSLGLAKNILAVRLRKLVSRGIMQTVPASDGSAYHEYVLTEKGQRLHLVLMAIWQW
                     AETSLFAPGEMQGVLCDQQNGEPVAPMELRSRDGRVLRIGDTILRRDLRTGASPKKHR
                     SGAKRRPPSKRSTALNATR"
     gene            9103..10044
                     /locus_tag="VGP50_12140"
     CDS             9103..10044
                     /locus_tag="VGP50_12140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8028174.1"
                     /translation="MAAVAPSYGWLVASRILLGLGAGTFFPTASGYAATAAAPALRGR
                     ALALIYSGLTIATIVGVPLGTAVAARLGWRATFIGVAVLSVIALVGIAAGLPQVQNPP
                     VASLADRMKVAARSDMVSVLVLTVIGFAGFQVVYPYFAPLLTRMAGFEGDGIAVMLLV
                     YGIAGAAGNFLGGYGADKWNVRRYLLIVLAVLVADYCAFAFFAGRELPIGLARPAAAA
                     LVFIWGLAGWMFPATQQYRLVRLAGPLAPIALSLNSSAIYLGTSIGAVLGSLVLSSRP
                     VASLGLAAAACYVFAFLWLALSREKAAGAVVESRAAE"
     gene            10080..>10652
                     /locus_tag="VGP50_12145"
     CDS             10080..>10652
                     /locus_tag="VGP50_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8028175.1"
                     /translation="MVNLLERRTVLEAFALTAAASAFFGSRRAMADAAPSPTTLSVPN
                     EGKTMPSELTHRTVTLNGLRFHIAEQGQGPLVLLCHGWPESWYSWRHQLPAVADAGFH
                     AVALDMRGFGQTDAPEDMSAYTVVHYVGDLVQLVSALGEKQAIIIGHDWGATVAWTAA
                     LLRPDLFRAVVALSVPYRPRGPVAPLKALRD"
BASE COUNT         1749 a         3483 c         3724 g         1696 t
ORIGIN      
        1 ttcctttctg agtctcgaca accagaaagt acacggcttg tgccgatgtg gccgggcatg
       61 cgcctgcgac ttatcgactc attccccgga cagccctgcg caccgcggga gagggagggg
      121 cccgccgcgc aggcggcggg agggtgaggg gaagtttcaa gcaaaggcgc tttgcctgac
      181 gcgccccctc accttcccac tgcttcgctg aagcttcgca gtgggcccct tcctctcccg
      241 cgatgcggga gaggggatgt ttggcttggc ccagaggaca gggcagcagt cgtgtctccg
      301 ccgcgttaga ctgacgaggg agcttcgcag gagaccgagc atggcgatcg aatcgatcaa
      361 ccccgcgacc ggggacgtgg tggcacgcta cgaggagatg gcgccggcgg ccgtggccga
      421 catcgtcgcc aaaacccatg aggcattcct gggctggcgg cgcacgtctt tcacgacacg
      481 cgcgcaggcg atgcgcaagg cggcggagat cctgcgcagc gacgccagcc actatggaaa
      541 gctgatggcg caggagatgg ggaagccgct ccgcgacggc atcgccgaga cgcagaaatg
      601 cgccaccggc tgcgacttct acgccgagca tgccgcgcgc ttcctggcgc cggagccggt
      661 cgccacagag gcgcgcaaat gcttcgtcac cttccagccg ctgggcgtcg tgctggcggt
      721 gatgccgtgg aacttcccgt tctggcaggt cttccgcttt gccgcgccgg gactgatggc
      781 gggcaacgcg gcggtgctca agcacgcctc gaacgtgccg ggctgcgcgc tggccatcga
      841 ggacgtcttc cgccgcgcgg gattccccga gaacctgttc cgcacgctca tgatcggcag
      901 ccgccaggtc gaggggatca tcgagcatcc gctcatccgc gcggcgacgc tgaccgggag
      961 cgggcccgcg ggccgggcgg tggcgagcaa ggccggcgcg atgctgaaga agaccgtgct
     1021 cgagctcggc ggcagcgatc cctatctcgt gctcgaggac gcggacatcg accaggcggc
     1081 ggcactcagc gccaagggac ggctgatcaa tgcggggcag agctgcatcg ccgccaagcg
     1141 cttcatcgtc ctcgacaagg tccgcgccgc gttcgaagag cggttcgtcg agcgcatgcg
     1201 cgcggcgcga atgggcgacc cgctgcagga aggcacggaa gtcggtccgc tggcgcggca
     1261 tgacctgcgc gacgcgctcc accagcaggt ggaggcgagc gtggccaagg gcgcgcgctg
     1321 cctgctcggc ggccggattc cgaaggatcg cggcgcctac tatccgccga ccgtgctgac
     1381 cgatgtgcgc aaaggcatgc ccgcctatga cgaggagatg ttcggtcccg tcgcttccgt
     1441 catcgccgtc aaaagcgaag aagaggcgat cgccgtggcc aatgattcgc cgttcggact
     1501 gggcggcggc atcttcagcc gcgacctcgc gcgcgccgag cgcatcgccg ccgaggagat
     1561 cgagagcggc gcggtcttcg tcaacgagac cgtgcgctcc gatccacgct ggcccttcgg
     1621 tggcgtcaag gaaagcggct acgggcgcga gctgtcggcc tacggcatca aggagttcgt
     1681 caacatcaag agcgtctata tcgcctgacg gccgccacat cgtcatcccc gcgaggaaga
     1741 cgaaggaaga atggctctgc gtgaatgggg cgtcacccgc aacggctcat tggtcacggg
     1801 caccagggag agaaccatga caagcgcagt cgtccagcag cagaccggca ggaggctgcc
     1861 gtcatgagca gcgattacga cgtcatcgtc atcggcggcg gatcgccggg cgagcactgc
     1921 gccggcgcgc ttgccgcggg cggccttcgc gtcgcactgg tcgagcgcga gctgatcggc
     1981 ggcgagtgct cctactgggc gtgcatcccg tccaagactc tgctgcgacc gggcgaagcc
     2041 gcgcacggcg ccaacgaagc ggcggcgacg gcgcaggtcg acgtcagcgc gacgcttgcc
     2101 tggcgcgact tcatggtctc gaactactcc gattcgggcc agcagcgttg gctcgccgag
     2161 catggcatcg ccctgttgcg cggtcgcggc cggctggccg ggacgggcgt ggtcgaggtc
     2221 gacggcgcgc gccacacggc cgatcacgtg gtggtggcga cgggtgccga gccgatcatc
     2281 ccgcccgtgc cgggcctgcg cggcctcgag ggcatctgga cgaaccgtga ggcgaccggc
     2341 atgaaggcgg ttccccgccg gctgctcgtg ctcggcggtg ggccggtcgg cgtcgagatg
     2401 gcgcaggccg ttcgtcgcct cggcggcgcg gtggttctcg tcgagggtgc tgcgtatgtg
     2461 ctcccccacg agccccggcc tctcggcgag gcactcgccg acgtcttgcg ccgcgacggg
     2521 atcgagctcg cgctgggcgc gcgcgcgacg gcggcgcgcc gcgaaagcga gcactacgcg
     2581 ctcgacctcg acgacggccg gacgctgagc ggcgaccggc tgctcgtcgc caccgggcgg
     2641 cgcccgcgcg tcgacgggct cggcctcgag actgtcggcg tcgcagccaa caagcgcggg
     2701 atccccgtcg atgcgcagct gcgcgcgggc gagcggctct gggcgatcgg cgacgtcacc
     2761 ggcatctggc cgctgaccca tgtcgggaag tatcagggcc gggtcgtcgc ggcgaacatc
     2821 ctgggcgaga agcgcgaggc caactacgag gccgtgccgc gggtgaccta caccgatccg
     2881 caggcggccg ccgtcggcgc ggccgaggcc agctacagcg caaccgcccg cctggcggag
     2941 gtggcgaaga ccgccaccta cacgcgcgcc tacgcccagt cgaacgggtt cctgaccctg
     3001 ctcagcgacg gcgcgcggct gaccggcgcc tatgcgctcg gccccgaggc cggcgaatgg
     3061 ctgcagcagg ccacgctggc gatccgcgcg cgcgtgccgc tcgcggtgct gcgcgacacc
     3121 atccagccct tccccacctt ctccgagatc tatctcgacg cgctgaaagc cctcgatggc
     3181 cagatcagga cagcgcgccc ggcggccggg gcggcgacaa aggcgcgata gctcacatct
     3241 cactatgttg ttttggcggt cccgacaatt tgcagatttc cgtctcggtc cacctttctt
     3301 gattcggaag ggcgggagtt ctgcaacaac ggatgaacgg cacaggacct ccacccaagc
     3361 ctgacgaatg ggtattcgag atcgacgaat ttgcaagaaa aatgggcgag acgggctgac
     3421 gccgtgagcc agcccgtctc ggcgctgtca tcttgggtaa caacaatcac cgcgccggga
     3481 acagcggcga agcgacgacg caatggcagg gcgagcgtct ctaccgtgac ggcaggggcg
     3541 tcggcgcgcg atacggtaaa actgtgataa tacgcgcagg gcatccggcg agcgcgctcg
     3601 cgggcgatct tcccggcatc gcggaccgcc ggcgggacaa ggtccagcca gttgacctgt
     3661 tggaggtctc ggccgaaacg cttgcgcagg cgctcacccg cgaactcgac agccatatga
     3721 tacggcaatc ccagatcgat catgccgagg gagtcgaaga tgggcgccaa ctcgttcgga
     3781 tcgatttcgg cttcgagcgg caccagcgcg ccaccgcgga tatcgagcca atgccgaatc
     3841 aggccgcggg cgaagtcgag ccgcgtatcg tagagattca tgtccgcctc tctttgttgt
     3901 agcggacgcc gaggccgcgg tgacgagcgc ggcctcggct tgctgaaagc cggccagttg
     3961 cgcatcgatc tccgcgatca acggctgcgt ttttccattc atgttcagaa taaaaattag
     4021 aaaccagccg cgaatattga cttatggagt tttagcgcga aatcaagagt ccgccgaaat
     4081 gcggctgggg cgtcacgacc tctcgctcag gcgcgcggcc aaattgaaat tcgagatgga
     4141 tgccagcgtc gttgttgttt cgcgcatccc ccgcccctct cccgcatcgc gggagggtgg
     4201 cgcgggcgaa gcgcgcgctc gcgacgacgc ctatggcgtc gcgcgggggt gaggggcgct
     4261 tcagaaaaaa gcgctgtgcc tgacccgttc cctcaccctc cctctcccgc gatgcgggag
     4321 aggggaattt atttatgcca gctgcgcggc gagccggtcg gcgagtgccg ccagggcgtc
     4381 gctcatcgcg gcgacgagat cgggggtgcc gggcgaggcc gggcgcgcgg tgatccgctc
     4441 cgcaccgaga cggggggcgc cgctgccgcg ccggatggtc cagcgcgaat tcagcacgac
     4501 catcccgtcg ggatcggcat cgaagcggtc gatatagagc tcgaggctgg tatcggcagg
     4561 cacggtcgct gcgctatcgc cgacgaagac ggtgccaccg ggaaggcgcg ccgccaagtc
     4621 ctcggcgagg acccgtccga ccatgtcggc catgccttcg gcccagacct cgaactcggc
     4681 gacggcgagc tcatagccgc tgtggtggcg cacgatctcc ggctgatcaa ggtatttggc
     4741 aatgccgatt ccggcgatgg cgacccgccc ggcgaaggac ggcgccgtgg ccgcgcccgc
     4801 gccgcgcggc gccagcagga agcgtcggga gggcggcgac gtgcagccgc tcgtcagtgc
     4861 cgcgaaagcg aggccggcga tgagaagccg gcgcgacagc gaggctgggc gcatcgtcgc
     4921 tctatcggcc gcggaccagg gcttcaggat gctgctcgag cgagtcggcg agcgccttga
     4981 ccgagcgggc ggcgtcctgg aactggcgca gcagggccgt caagtcgcgg cgcacctgcg
     5041 aatcgtcgcc atagccgctg ttgaccgagg caagcgtttc gtcggcgtgc gcgatcgcgt
     5101 cctgtgccgc gtccgaggtc ttgcgcaggc tggcgagcag cggtcccgcc tgggtgtcgg
     5161 cattgcgcat caggctctcg gcggccgcca gcgcctggct cagcgagtgc atggactggc
     5221 gcagctccgg catgtcggcg acgcgctgat aggattgaag cgtggcatcg agatcgcgca
     5281 ccagttggtc gaggggcaag gcggagatct tgtcgagcac ggtggtgagc gaggtggtga
     5341 tgccctcgag gtcgcccggc accgtcggaa tcctgggatc ggggccgctg gtgtcgagtt
     5401 tgtccggcgg tgcgcgcgga aagatgtcga gcgcgacata gagctgcccg gtgatcagac
     5461 tcgaagagcg cagctgcgcg cgcatcccgt gctcgatgag caccgcggtg agcgacgccc
     5521 ccggcggcaa cggcgtgtcg gcggattgct gcagatgctg gcgctcgagc tcgagcgtga
     5581 ccggaacccg gacgatgttg ctgcgcggat cgaattcgag atgcacatcg acgacgcggc
     5641 cgaccttgac gcccttgaat tcgacgggcg caccgggatc gagcccgtgg accgagcctt
     5701 cgaactcgac gcgatagcgg gcgtgaacgg tgtaggccga ttcggcgacg ctctcgtggt
     5761 cgtcgagcag ggcgaagacg gtgccttcct tggcgggctc ggcgccttgc gccgtgtcgg
     5821 gcgtctcgaa ggcgatgccg cccgagatca ccgcttgcag cgattcgatc tggaacttga
     5881 agccctggcc gccgggcgtc agcgagatcc ccgacgcgct ccagaagcgc gtgccctcgc
     5941 gcaccagggt gtcataaggg gcgcggacga aggcgtggac cgcgatgtct tgctcggctg
     6001 cggcaaaatc gtagccgagc acctcgccca cctggatgcc ccggaaatag atcggcgagc
     6061 ctgggcccag cgcgcccagc ttcggcgtca tcagcacgaa ctcgcggccg gggacgtcgg
     6121 cgcgcaccac cggcggcagc tcgaggccct tgaacgcctc ggcgtaggcg ccggcgccgg
     6181 ggtccatctc gatatgcgcg cccgagacca gcgtctcgag gccggagaaa ttcgtgaggc
     6241 tgaggcgcgg gcgcaccacc cagaagcgcg ttccctcgtt caggtgacgc gacgccgtct
     6301 tcagcatccg cgcgcgcacc acgacatgcg agagatcctg gctgagctcg acggtctcga
     6361 cgacgccgag ctgcacgttg ttgtgcttga tctgggtctt gccggcctcg aggccgtcgg
     6421 cggtggcgaa ggtgatggtg atgcgcggtc cctgagcggc gagcgtcgta taaacgagcc
     6481 agagggcgac caaggcggcc acggcgggga cggcccagat cggcgagacg tggggccggc
     6541 gcttcaggcc gggcggcagg agcttcacgg attctctccg gcgctgtccc acatcaggcg
     6601 gggatcgaag gcggttgccg ccagcatggt gacgacgacg acggcggcga aggcgagagc
     6661 gcccgcgccc ggttcgaccg aggcaattgc gccgagccgc accagcgcca ccagcaccga
     6721 gagcatgaag atgtcgatca tcgaccagcg cccgatgccc tcgacgatgc gatggagccg
     6781 cgtgcgctcg tggatgcgcc agcgcgagcc gcgctgcgtc gtcaccagca acagcaggag
     6841 cgagagcagc ttgagcacgg gaacagtgat gctggcgaag aacacgagca gcgccagcgg
     6901 ccacatcccc gacacgatca gatgctcgac gccgctcagg atggtgtccg gcgcgccatt
     6961 gccgaaggag atcaccgtca tgaccgggag atagttggcc ggcagataga agatcaaggc
     7021 cgcgatgacc agcgcccagc agcgcgcgag gctctcttcc ttgcgcgcat ggagcgccgc
     7081 accgcagcgc ggacagcgcg gatgcccgcc ggccgcgacg ctcaccagcg cgcaagcgtc
     7141 gcagcgtgcg agcttgcccg cgccgccgct ttcgacgccg ggggccagcg ccttccagat
     7201 cgcttcggca tcgagagtga cgtcgagggt cgccatcacc agcatcagtg cgaccagcgc
     7261 atggagcgcg gcgccgatct cgatcgcggc gatgtcgccg agcttcacat aggcgacgaa
     7321 gacgccgagc agatagactt cgatcatcgc ccaggggcgc cagcgctcga gccagcggaa
     7381 gagcgtccgc gcatgcggcg gagcgggccg cgagagctgc agcagcacgt aagccgtacc
     7441 cccgagacgc gccagcggca ccacgatggt ggtgagcatg atgagagccg ccagcggcca
     7501 gagcccatcc gcggccagcg ccagcgcgcc gctcgccaga ctcgccacct cgacccggcc
     7561 ctcgatgcgg atcgacatca gcggcatcag attggcgatc gccgcgagga tcaggccggt
     7621 gaggctcagc gccatgctga cctcgcgcga agcggcggcg cgcggcagca gcagcgcgcc
     7681 gcagcggcga cagagcgccg catgccccgc cggcgtctcg gccgcggcgt ggcgcaaccc
     7741 gcaatggcgg cattcgacga tcatggagga atggccggcg tcatcgaccg gtgcaacggt
     7801 catcgctcct cggacaagcg gttggtctct tcgcgggagt cgcccccgac ggagcttcgc
     7861 gcagaatggg ccggcggcgc aagctgcgct cgctaagcta gccgttcggc taggggagag
     7921 ggagaaagag tggcgaccga tgttacgccg ccttggagaa atgatcgtcc ctccgcgtcc
     7981 tcggcgaagc ctccgcgtcc tccgtgttca tcttttcctg tgctgccgct cctcaccggt
     8041 ctggtcgtcg acgagctcct tgaacgccgc gagcgccatg gcccagcgca cgggcaaagc
     8101 cgcttggact tggcctaggc gatgaccaga tattggtata acattgccac cagtgtcggc
     8161 gagaaggcaa atcgagggac gcggcggtga aagggaaacg caggagtttt gccgcggacg
     8221 cgtgcccgat cgcacggacc gtcgacacga tcggcgattg gtggtcgctg ctcatcatcc
     8281 gcgatgcggc gctcggggga aagcgccgct tcggggagtt tcagaaaagc ttgggactgg
     8341 caaagaacat cttggcggtc cggctgcgta aactggtgtc gcgcggcatc atgcagaccg
     8401 tgccggcatc ggacggcagc gcctatcacg aatatgtcct caccgagaag ggccagcgcc
     8461 tgcatctcgt gctgatggcg atctggcaat gggccgagac ctccctgttc gccccgggtg
     8521 aaatgcaggg tgtcctgtgc gaccagcaga atggcgagcc cgtcgcgccg atggaattgc
     8581 gctcgcggga cgggcgcgtc ctgagaatcg gcgacacgat attgcggcgt gacctgcgaa
     8641 cgggcgcaag tccgaagaag catcgatcgg gcgcgaagag acggccgccg tcaaagcggt
     8701 cgacggccct gaacgcgaca cgataagttc gacgatacgt tcgccacgag gcaattccgg
     8761 ccgtcaaagc ccgaagcgtg ggtccaacca aacataagtt gcatttttaa actgcatgtc
     8821 gcatcctgtc gatggttgca ttattacacc atcgtgaggg tacgatgccg cgctatctgg
     8881 tctggttgat ggtcggcgcc tttgccatcg gcaccgaggg cttcatgatc gcgggcatcc
     8941 tgcctctgat cgccaccgat ttacgtgtct cgaccgctct tgcggggcag ctcgtgaccg
     9001 cgtttgcgct cgcctacgcg atcggctcgc cgaccctgac ggtcgcgacc ggtcgattcg
     9061 atcggaaaaa ggttctcttg tggtcgatac ttttgttccg tcctggcggc ggtcgcgccc
     9121 agctatggct ggctcgtggc ctcacggatt ctgctcggcc tcggcgccgg gaccttcttt
     9181 ccgacggcca gcggctatgc agcgacggcc gcggctccgg cgctgcgcgg acgcgcgctg
     9241 gccctgatct attccggcct gacgatcgcg acgattgtcg gcgtgccgct ggggacggcg
     9301 gtcgccgcgc ggttgggatg gcgtgcgact ttcatcggcg tcgcggtgct gagcgtgatc
     9361 gcgctcgtcg gcatcgccgc cggtcttccg caggtgcaga acccgccggt cgcgagtctc
     9421 gccgaccgga tgaaagtagc ggcccgttcc gacatggtgt cggtcctcgt gctgaccgtc
     9481 atcggctttg ccggattcca ggtcgtctat ccctattttg cgcctctgtt gacgcggatg
     9541 gcgggcttcg agggtgacgg catcgccgtc atgctgctgg tttacgggat cgcgggagcc
     9601 gcgggtaatt ttctcggcgg ctacggtgcc gacaagtgga atgtgcggcg ctacctgttg
     9661 atcgttcttg ccgtcctggt cgccgactac tgcgccttcg cgttcttcgc cggtcgtgaa
     9721 ctgccgatcg gcctcgcccg tcccgccgcc gctgccctcg tcttcatctg gggactcgcc
     9781 ggctggatgt ttccggccac ccagcaatac cgcctcgtcc ggctcgcagg accgctcgca
     9841 cccatcgcgc tctcgctcaa ttcgtcggcg atctatctcg gcacgagcat cggcgcggtg
     9901 ctcggctcgc tcgtgctgtc atcgcggccc gtggcatcgt tgggcttggc ggcggcggcc
     9961 tgctacgtct ttgccttcct gtggctcgcc ttgtcgcgcg agaaggccgc cggcgcggtg
    10021 gtcgaatccc gcgccgccga gtgaacttcc ggcgcggcgt gacccaagga gaccccagga
    10081 tggtcaattt actggaacgc cggacggtgc tagaggcatt tgcgcttacg gcggctgcgt
    10141 cggcattctt tggatcccgt cgcgccatgg ctgacgccgc gccgtcgccg acgaccttgt
    10201 ccgtgccaaa cgaggggaag accatgccaa gcgagctgac gcatcggacg gtaacactca
    10261 acgggctacg ttttcatatc gccgagcagg gccaggggcc gctggtgctc ctttgccacg
    10321 ggtggcccga gagctggtat tcgtggcggc accagcttcc cgccgtcgcc gacgccggct
    10381 ttcacgccgt tgcactggac atgcgcggat ttggccagac cgatgcgccc gaggacatgt
    10441 cagcgtacac ggtcgtgcac tacgtgggcg acttggtgca actcgtctcg gcgcttggcg
    10501 aaaaacaggc catcatcatc ggccatgatt ggggcgcgac ggtggcgtgg acggcggcgc
    10561 tgctccggcc cgacctcttc cgggctgtcg tcgccctgag cgtgccctat cgcccgcgcg
    10621 gccccgtcgc cccgttgaaa gcgctcaggg ac
//
LOCUS       DASYNS010000389         6912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1969131, whole genome shotgun sequence.
ACCESSION   DASYNS010000389 DASYNS010000000
VERSION     DASYNS010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1969131"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VGP50_12150"
     CDS             complement(<1..1206)
                     /locus_tag="VGP50_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV8028176.1"
                     /translation="MQRANSVSSRDIAFTLHPYTNLKKHETEGPLVVTEGRGIYVYDE
                     NGKEYIEGLAGLWCTALGFSEERLVDAATRQLKRLPFYHLFNHKTHDVGAELAERLIA
                     LMPVEMSKVFFNNSGSEANDTAVKLVWYYNNALGRPRKKKIIGRMKGYHGVTVATASL
                     TGLPNNHRDFDLPIANIRHADCPHHYRFAKPGESEEEFATRLAESLEAQILREDPDTV
                     AAFIAEPVMGAGGVIVPPRTYFEKIQRVLRKYDMLLIADEVICGFGRTGNMFGSETFG
                     LSPDIITVAKALSSAYLPISATVISEPIYRALVAQSEKIGTFAHGFTYSGHPVCCAVA
                     LETLKIYEERDIVGHVRAVAPQLQQGLKRFADHPLVGEVRGVGLIAAVELVRDKATKA
                     PFDAKDGVGA"
     gene            1423..1683
                     /locus_tag="VGP50_12155"
     CDS             1423..1683
                     /locus_tag="VGP50_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028177.1"
                     /translation="MRRDFVGWRRRNHLVIVLCNITHRTTGMADNDALAIGGDIDMDR
                     VIIDPDYRRVVIAMLRQTRSRSERDAAAPRAAEPPASARGNG"
     gene            complement(1680..2612)
                     /locus_tag="VGP50_12160"
     CDS             complement(1680..2612)
                     /locus_tag="VGP50_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8028178.1"
                     /translation="MTLQPAAALSPRRLGLLPKHWRRAAAQPRVAVSAGILILLVLGV
                     AAAPLVAAVLGTDPQNVDLYQRLAAPSLAHPLGTDELGRDLLVRLLEGGRVSLLVGIT
                     SALAAAGIGTTVGLLAGYGGGRRDALLMRVTDGFIALPLLPLLVVLAAIDLRKLGLSS
                     GLIQSEDMSLYRIIVLVALVGWTTVGRLVRGATLSLKAQDFVRAALSLGASPARIMLV
                     HILPNLASPIIVATTLSVGQLILLESVLSFLGLGIEPQIASWGNMLMGAQETIELAPQ
                     LAHYPGLLIFVTVIACNLLGDGLQDALDPRAVRR"
     gene            complement(2620..3630)
                     /locus_tag="VGP50_12165"
     CDS             complement(2620..3630)
                     /locus_tag="VGP50_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8028179.1"
                     /translation="MHWPSLSPLEGEGWGGGWPLTRFIAARLLQVLAVLAVMSFIIYG
                     LIGLMPGDPIDLMLTADPHLTSADAARLKALYGLDQPLAKRYATWAQAALSGDFGYSR
                     LYATRVTAALLPRLGNTGLLMGTSFLVALGLALPLGVLAARRPGAAFDVAANLAAFAG
                     ISVPPFWLALMLILVFAAGLGWLPAGGVATIGEGGVVDRLRHLVLPVATLSAASLGSY
                     LRFMRAAMVEAMRQDYVRTARAKGASETRVLWGHALRNALIPVITVVALSFGTLFSGA
                     LITETMFAYPGMGKLIYDAILGNDFNLALAGLLFATLLVLLSNLAADIAYAAADPRIT
                     YR"
     gene            complement(3689..5362)
                     /locus_tag="VGP50_12170"
     CDS             complement(3689..5362)
                     /locus_tag="VGP50_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV8028180.1"
                     /translation="MRRLWAHLLGLLLWAAAHGAAARPADELAIGIIQFPSTLHPSID
                     AMVAKTYVLGMVRRPITTYDAEWRLVCMLCTTLPTIENGLAVPFDLPDGKRGIRVTYT
                     LRADARWGDGVPVTTADVLFTQEVGSNPKSGVGDAELYRRITAIEVKDDKTFTMVIDK
                     LDFDYAAIGDFEVLPAHLERAAFADPAAYRFHTLYDTDPTNPGLYFGPYRITELAPGS
                     HIVLERNPTWWGPPPAFRRIVVWAVENTAALEANLLAGGLDMVAGEIGFSLDQALAFE
                     KRHGDAFTVLYKPSLSYEHIELNLDDPALADKRVRQALLYGIDRDAISRQLFAGRQPV
                     AQSFVSPLDWIFTADVPRYPYDPAKARALLDAAGWRGGDGKVRRNEAGEKLSLELATT
                     AGNRVRELIEQVLQSEWRQIGVEIHLKNQPARLLFGESVAKRHFTMALFAWTSAPENV
                     PRSSLRSDEIPTAASGWRGQNYGGFKNAEADRLIDAIEIELDRHRREALWHRLEALYA
                     EELPALPLFFRADPYVLPKWLKGVTPTGHQFPTTLWIENWHVVGRDTGS"
     gene            5497..5991
                     /locus_tag="VGP50_12175"
     CDS             5497..5991
                     /locus_tag="VGP50_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028181.1"
                     /translation="MKHLLSAIAVATLMSSVPAWAQTSASDPSQQPIPQAGSSGQGDS
                     SAMGSGTSTGKAHHKAAKRGGGAPQDNMAEELNQQELDRVMHNGGEASGSGMQPDGVT
                     GTSGGMSGTTAAPSGTSNGSLPSSGAPQGYQPDNAGSRAGGAEIGQPSPGTLEEHPGA
                     SSKQ"
     gene            6130..>6912
                     /locus_tag="VGP50_12180"
     CDS             6130..>6912
                     /locus_tag="VGP50_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV8028182.1"
                     /translation="MQKHEWWPGAVIYQIYPRSFYDSNGDGVGDLAGVAAKLDYVASL
                     GVDAIWLSPFFVSPMRDFGYDVADHCAVDPVFGTLDDFDRVRSRAHEKGLKLIVDLVC
                     GHTADRHEWFRESRARADDPRSDWYVWADARPDGTPPNNWLSVFGGSAWSWEPRRRQY
                     YLHHFLAAQPTLDLRHPAVIDALLAVAEFWLARGVDGFRLDAIDFLLHDPVLRDNPPL
                     PPADGKMPVKPFALQHHCHDMMQSEVPELLRRLRALADRRGGI"
BASE COUNT         1202 a         2223 c         2428 g         1059 t
ORIGIN      
        1 cgcgccgacc ccgtccttcg cgtcgaaggg cgccttcgtc gccttgtctc tgaccagctc
       61 gacggcggcg atcagcccga cgccgcggac ctcgccgacc agcgggtggt cggcgaagcg
      121 cttcaagccc tgctgcagct gcggcgccac cgcgcgcaca tggccgacga tgtcgcgctc
      181 ttcgtagatc ttgagcgtct cgagcgccac cgcgcagcag acgggatgtc cggaataggt
      241 gaagccatgg gcgaaggtgc cgatcttctc gctctgggcg acgagcgcgc gatagatcgg
      301 ctcgctgatc accgtggccg agatcggcag ataggccgag gacagcgcct tcgccaccgt
      361 gatgatgtcg gggctgagac cgaaggtctc gctgccgaac atgttgccgg tccggccaaa
      421 gccgcagatc acttcgtcgg cgatgaggag catgtcgtat ttccgcagca cccgctggat
      481 cttctcgaaa taggtgcggg gcggcacgat gacgccgccg gcgcccatca ccggctcggc
      541 gatgaaggcg gccacggtgt ccgggtcctc gcgcaggatc tgcgcctcga ggctctcggc
      601 cagccgcgtc gcgaattcct cttcgctctc gccgggcttg gcgaaacggt agtgatgcgg
      661 gcagtcggca tggcggatgt tggcgatcgg caggtcgaag tcgcggtggt tgttgggaag
      721 gcccgtgagg ctcgccgtgg caacggtaac gccgtgatag cccttcatcc gcccgatgat
      781 cttcttcttg cgcggccgcc ccagggcgtt gttgtagtac cagaccagct tcaccgcggt
      841 gtcgttggcc tcggagcccg aattgttgaa gaacaccttc gacatctcga ccggcatgag
      901 ggcgatcagc cgctccgcca gctcggcgcc aacgtcatgg gtcttgtgat tgaagagatg
      961 gtagaaaggg aggcgcttca gctgccgggt ggccgcatcg acaagacgct cttcgctgaa
     1021 gccgagggcg gtgcaccaga gtccggccag gccctcgatg tactccttgc cgttctcgtc
     1081 atagacatag atcccgcgcc cctcggtgac gacgagcggc ccctcggtct cgtgcttctt
     1141 gagattggta taggggtgga gcgtgaaggc gatgtcacgg ctcgagaccg agttggctcg
     1201 ctgcatgggc gcgttcctcg aatgagacca gacgccagta tacaggccgc tcgctgcgtg
     1261 gcacaggcgc tcggtcgaca ggccgctcgg tcgccagcga gggcgagcct gcgaccaccg
     1321 gtcgcacccg ccggcgcaat gccttgatcc gtcatatgcg ctggcaagcc ttcgcggctc
     1381 atcaaccggg atatcaacag ggttttcccc agcgcctaaa aagtgcgcag agatttcgtt
     1441 ggctggcgcc ggcgcaacca tttagtgata gtgctctgca acatcacgca ccggacgaca
     1501 ggcatggcgg acaacgacgc tctcgcgatc ggcggcgaca tcgatatgga tcgcgtcatc
     1561 atcgatcccg actaccgtcg ggtggtgatc gcgatgttgc gccagacgcg ctcgcgcagc
     1621 gagcgagatg cagccgcccc gcgcgcggct gagccgcccg cctcggcgcg gggaaacggt
     1681 taacgccgca ctgcgcgcgg gtcgagcgcg tcctgcaggc cgtcgcccag caggttgcag
     1741 gcgatcaccg tcacgaagat gagaaggccc ggatagtgcg cgagctgggg cgccagctcg
     1801 atcgtttcct gagcgcccat cagcatgttg ccccagctcg cgatctgcgg ctcgatgccg
     1861 aggccgagga agctcagcac ggattcgaga aggatgagct ggccgaccga aagcgtcgtc
     1921 gccacgatga tcggcgaggc gaggttcggc aggatgtgga ccagcatgat ccgtgccgga
     1981 cttgcgccga gcgagagcgc cgcgcgcacg aagtcctggg ccttcagcga cagcgtggcg
     2041 ccgcgcacga gccgccccac cgtcgtccaa ccgacgagcg ccaccaagac gatgatgcga
     2101 tagagactca tgtcttcgga ctggatgagc ccggacgaca ggcccagttt cctcagatcg
     2161 atcgcggcga gcaccaccag cagcggcaac agcggcaggg cgatgaaacc gtcggtgacg
     2221 cgcatcaaca gcgcatcgcg cctgccgccc ccataacccg caagcaggcc caccgtggtg
     2281 ccgatgccgg cggcggcgag cgcgctggtg atgccgacga gcagcgagac gcgtccgcct
     2341 tcgaggagcc gcaccagaag gtcgcgcccg agttcgtccg tgccgagcgg atgggcgagc
     2401 gacggtgcag cgaggcgctg gtaaaggtcg acgttctgcg gatcggtccc gaggaccgcc
     2461 gcgacaagcg gtgccgccgc gacgcccaac acgagcagta tcagaatgcc ggcgctcacg
     2521 gcgacgcgcg gctgggccgc ggcgcggcgc caatgcttcg gcaaaaggcc aaggcgcctc
     2581 ggacttaggg ccgccgcggg ctgcagtgtc atggtccact catcgatagg tgatccgcgg
     2641 gtcggccgcg gcataggcaa tgtcggcggc caggttcgag aggagcacga ggagcgtcgc
     2701 gaagagcagg ccggcgagcg ccaggttgaa gtcgttgcct aggatcgcgt cgtagatcag
     2761 cttgcccatg ccggggtagg cgaacatcgt ctcggtgatg agcgcgcccg agaagagcgt
     2821 gccgaaggac agcgcgacca cggtgatgac ggggatgagc gcgttgcgga gcgcgtggcc
     2881 ccaaagcacg cgcgtctcgc tcgcgccttt ggcgcgcgcc gtgcgcacat agtcctggcg
     2941 catcgcctcg accatggcgg cccgcatgaa gcgcagatag ctgcccaggc tcgccgcgct
     3001 cagcgtcgcg accggcagga caaggtggcg caggcgatcg acgacgccgc cttcgccgat
     3061 ggtggcgacg ccaccggcgg ggagccagcc caggcccgcg gcgaagacga ggatgagcat
     3121 cagcgcgagc cagaagggcg gaaccgagat gccggcgaag gcggcgagat tggcggcgac
     3181 atcgaaggcc gcgcccgggc ggcgtgccgc caggacgccc agcggcagcg ccaagccgag
     3241 cgccaccagg aagctcgtcc ccatcagcag gccggtgttg cccaggcgcg gcagaagcgc
     3301 ggcggtgacc cgtgtcgcat aaagccgcga atagccgaaa tcaccggaga gcgccgcctg
     3361 cgcccaggtg gcgtagcgct tggcgagcgg ttgatcgagg ccgtagagcg ctttcagccg
     3421 ggcggcgtcg gcggatgtca gatgcgggtc cgcggtgagc atgagatcga tgggatcgcc
     3481 cggcatcagg ccgatgagcc catagatgat gaaggacatg acggcgagca ccgccagcac
     3541 ctggagcagc cgtgccgcga tgaaacgagt cagtggccac cccccacccc atccctcccc
     3601 ctcaaggggg gagagggaag gccagtgaat cccctcctcc cttgaggggg agggtcaggg
     3661 tggggggtac attcggtcag aaccgcgctc atgatccggt gtcgcggccg accacgtgcc
     3721 aattctcgat ccagagcgtc gtcgggaatt ggtgccccgt cggcgtcacg cccttgagcc
     3781 atttcggcag cacataggga tcggcgcgga agaacagcgg cagcgccggc agttcttcgg
     3841 cataaagcgc ctcgaggcgg tgccacaggg cttcgcggcg gtggcggtcg agctcgatct
     3901 cgatggcgtc gatgaggcgg tccgcttcgg cgttcttgaa cccgccgtaa ttctgcccgc
     3961 gccagccgga ggccgcggtc ggaatctcgt ccgagcgcaa gctcgagcgc ggcacattct
     4021 cgggcgcgct cgtccaggcg aagagcgcca tggtgaaatg gcgcttggcg acgctctcgc
     4081 cgaagagcag acgcgccggc tggttcttga gatggatttc gacgccgatc tgccgccatt
     4141 cgctctgcag cacctgctcg atgagctcgc ggacgcggtt gccggcggtc gtcgcgagct
     4201 cgagggagag tttctcgccc gcttcattgc ggcgaacctt gccgtcgccg ccgcgccagc
     4261 cggcggcatc gaggagggcg cgcgccttcg ccggatcgta ggggtagcgc ggcacgtccg
     4321 cggtgaagat ccagtcgagc ggcgagacga agctctgcgc cacgggctgg cggccggcga
     4381 aaagctggcg gctgatggcg tcgcgatcga taccgtagag caaggcctgt cgcacccgct
     4441 tgtcggcgag cgcggggtcg tcgagattga gctcgatatg ctcgtaggaa aggctcggct
     4501 tgtagagcac ggtgaaggca tcgccgtgac gcttctcgaa agcgagcgcc tgatccagcg
     4561 aaaaaccgat ctcgcccgcg accatgtcga gacctccggc cagcaaattg gcctcgagcg
     4621 ccgccgtgtt ctccacggcc cagacgacga tgcggcggaa cgccgggggc ggaccccacc
     4681 aggtgggatt gcgctcgagc acgatatggg agcccggcgc cagctcggtg atgcgatagg
     4741 ggccgaaata gagaccgggg ttggtcggat cggtgtcgta gagcgtgtgg aaacgatagg
     4801 cggcgggatc ggcgaaggcg gcgcgctcga gatgcgcagg gagcacctcg aaatcgccga
     4861 tggcggcgta atcgaagtcg agcttgtcga tgaccatggt gaaggtcttg tcgtccttca
     4921 cctcgatcgc cgtgatgcgg cgatagagct cggcatcgcc gacgccgctc ttggggttgc
     4981 tcccgacctc ctgggtgaag agcacgtctg cggtggtcac gggcacgccg tcgccccagc
     5041 gcgcatccgc gcggagcgtg taggtgacgc ggatgccgcg cttgccgtcc ggcagatcga
     5101 acggcactgc gagtccgttc tcgatggtcg gcagcgtggt gcagagcatg cagacgaggc
     5161 gccattcggc gtcataggtg gtgatgggac ggcgcaccat gccgagcaca taggttttcg
     5221 ccaccatggc atcgatgctg gggtggagcg tcgatgggaa ctggatgatg ccgatggcga
     5281 gctcgtcggc cgggcgcgcc gccgcgccat gcgcggcggc ccacagcaga agtccgagga
     5341 gatgtgccca gagccgccgc atgtcgcgcg cgaggataac acggggcggc gttctgtcga
     5401 tcgccgccgg aacaaagcgc agacgtgact gttttgttgg gatcagggcc ggaaacggtt
     5461 cgcccggttt tcaaccgtca aggagaggga acagcaatga aacatctgct cagcgccatc
     5521 gccgtggcca cgctcatgag tagcgtcccg gcatgggcgc agaccagcgc gtccgacccg
     5581 tcgcagcagc cgattcctca ggccggctca tccggccagg gcgattcgag cgcgatgggc
     5641 agcggtacga gcacgggcaa ggcgcatcac aaggccgcga agcgcggcgg cggcgcgccc
     5701 caggacaaca tggccgagga gcttaaccag caggagctcg atcgcgtcat gcacaatggc
     5761 ggcgaagcct ccggcagcgg catgcagccc gatggcgtga ccgggaccag cggcggcatg
     5821 agcgggacga ccgcagcgcc ctccggaacg agcaacggca gcttgccgtc gagcggcgcg
     5881 ccgcagggct atcagcccga caatgccggc agccgcgcag ggggcgcgga gatcggccag
     5941 ccgagccccg gaaccctcga agagcacccg ggcgcgagca gcaagcagta gggaaggcga
     6001 gcgccctgcg gcgcatccgc cgcagggcgc cgccgcatgt tggcggccga tcgcggcgtt
     6061 ttgcagcttt cgaacatcgg agcggcagag cttcacattt tgagaaggaa tttgccgcat
     6121 accggcagaa tgcagaagca cgagtggtgg ccgggcgccg tcatctacca gatctatccg
     6181 cgcagcttct atgacagcaa cggcgacggc gtcggcgacc tcgccggtgt cgcggcgaag
     6241 ctcgactatg tggcgtcgct gggcgtcgac gcgatctggc tctcgccttt cttcgtctcg
     6301 cccatgcgcg atttcggcta cgacgtcgcc gatcactgcg cggtcgaccc ggttttcggg
     6361 acgctcgatg atttcgaccg cgtgcgttcg cgcgctcatg agaagggcct caagctgatc
     6421 gtcgatctcg tctgcgggca caccgcggac cggcacgaat ggtttcgcga gagccgcgcg
     6481 cgcgccgacg atccgcgcag cgactggtat gtgtgggcgg acgccaggcc cgacggcacg
     6541 ccgcccaaca actggctctc ggtcttcggc ggcagcgcct ggagctggga gccgcggcgc
     6601 cggcaatatt atctccacca tttcctcgcg gcgcagccga cgctcgacct gcgccatccg
     6661 gcggtgatcg acgcgctcct cgccgtggcc gagttctggc tcgcccgcgg tgtcgacgga
     6721 ttccgtctcg atgccatcga tttcctgctg cacgaccccg tgctgcgcga caacccgccg
     6781 ctcccgcccg cggacggcaa gatgccggtg aagcctttcg cgctccagca tcattgccac
     6841 gacatgatgc agagcgaagt tcctgagctg ctgcggcgct tgcgcgcgct cgccgaccgc
     6901 cgcggcggca tc
//
LOCUS       DASYNS010000390         5323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4310829, whole genome shotgun sequence.
ACCESSION   DASYNS010000390 DASYNS010000000
VERSION     DASYNS010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4310829"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1138
                     /locus_tag="VGP50_12185"
     CDS             170..1138
                     /locus_tag="VGP50_12185"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8028183.1"
                     /translation="MKLLAGAVALLVLATIPSWMGSYYVNIASQVLIWAILALGLNVL
                     VGYAGLVSLGHASLFGVAAYAVAWLVAAGWGHPGAALMAFGVTVVTSAMFAVLALRAT
                     GIGFLMITLAIGQILWGIAYRWISFTNGDNGINVASRPVLLGWPLGSPIHFYYFTLAV
                     FLFALAAIAVFVASPLGASLKGARDQPRRMIALGFNVWLIRFIAVIFSGVWSGIAGIL
                     FCYYNQFVSPNVLALGSSAEALLMVISGGSGTLLGPVAGAALVVVMKNVASAYIERWN
                     LVLGAIFVAIVLFMPEGLVPGSARLWRQGRAALRTRRPAAPIGSAP"
     gene            1135..1872
                     /locus_tag="VGP50_12190"
     CDS             1135..1872
                     /locus_tag="VGP50_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028184.1"
                     /translation="MTALRLRGLTKFFGGLRVAGGVDLQVEPGERRLIIGPNGAGKTT
                     LFNLIAGELKADAGSIVLFGRDITRVPSRGRAHLGLARTYQIITLFPRETLLRNVELS
                     LLALSPLRWNPLVPLARQAGLAARAREALARIGLEALAERRLADTSYGERRRVEIAMA
                     LAQRPRLLLLDEPFAGLSIDERKDVQKLLLAIPRDVTIVMIEHDMDVALELAERITLM
                     HFGEVIVEGTRAEVVAHPRTREIYLGA"
     gene            1862..2581
                     /locus_tag="VGP50_12195"
     CDS             1862..2581
                     /locus_tag="VGP50_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028185.1"
                     /translation="MAPDALMLGDIDAYYGDSHVLHQVSLRLREGRLLALLGRNGAGK
                     STCMNVAVGLLPPRRGAIELFGTLMTARSPEVIAAQGVALVPQGRRIFRSLTVRENLT
                     VAARKPRAGARHPWALDTVFATFPRLAERRAQVAGFLSGGEQQMLAIGRALMGNPRVL
                     LMDEPSEGLAPQIVAEVVATIRKLKEQGLSILLVEQNAKLAFDLADDVVILNSGRVVL
                     DLPVEQLKRDGSDLHQHLGVY"
     gene            2606..4906
                     /locus_tag="VGP50_12200"
     CDS             2606..4906
                     /locus_tag="VGP50_12200"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV8028186.1"
                     /translation="MTSGRHEAKWRGRVEDESLLRGAGRYAADAHIPGQATAWFVRSP
                     HAFASIRKVDTRAAEAAPGVLAVLTAGDMAATGVGNLSRPVPQSGRDGAPLRGSMRLA
                     LAEDRVRHVGEPVALVVAETRAAAQDAADLVEVEYDPLTPVVELRRAVALGAPQLHRE
                     APGNVALDWGLGATPENLAAIESVFGAAAHVSRISLVNQPIAVASLEPRGATAIYDPA
                     SGGYTLRCGSQGVMGLRDQLVAILGIEQDRLRLITEDVGGGFGMKSGAYPEYPALLVA
                     AKRIGRPVHWMSTRAEAFVTDQLARDTITEAALALDAQGRFLALRVKVLASMGAHVTA
                     VGAFIATSNFARCLSSVYRIPQIAAEIRCVYTNTVPTGPYRGAGRPEANYAIERVIDE
                     AARRTGIDRIDLRRRNLVTPAEMPYKTPIGVTYDSGDFPAIFEEALQRADVAGFAARR
                     KREAAAGKRCGLGISCFLEHAGGPPVEGAALAFPGGRSVALDLATGATGQGHATVFRR
                     LVAERLGLAEAQVTMRAGDTALGVQGSGTIASRNTMATGTASLRAVEAVIEKGLHQAA
                     RLLEAAEADIDYGAGSFRIRGTDRSVSLFDVAEQAKAAGEPLDSNVRVEVPQSFPNGC
                     HIAEIEIDPETGVLEIVAYVAVDDCGVALDPVLVEGQIQGGVAQGIGQVLMEHAVYDG
                     ATGQLLAGSFMDYAMPRADTVPDVQGTLYPVPCRTNPLGVKGAGEAGTTAALAAVMNA
                     IADAVPGIAIDMPATAEKLWRACRNL"
     gene            4962..>5323
                     /locus_tag="VGP50_12205"
     CDS             4962..>5323
                     /locus_tag="VGP50_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028187.1"
                     /translation="MRADAKRARRGAAPRRHFTVDIHCHVFTPEAERIVQSGFHPEME
                     PMFRYANEATRDVNRRQTEAIRGKLTSIETRLGDMDRLGIDVQAISPSPPQYYYWAAP
                     ELGREAARLVNNRIAEIVA"
BASE COUNT          768 a         1844 c         1782 g          929 t
ORIGIN      
        1 tgggcggcgc cggcagcctc ccgggcgcca tgctcggcag cctgatcatc gggttcctct
       61 acaatttcgg tcaggcgctg ctgccggagt tggcctacat cattctgttc ctgccgatgc
      121 ttctcattct cgtcatccgc ccgcaaggcc ttttcggcag gcctctgccg tgaagctgct
      181 ggcaggggcg gttgcactcc tggtgctcgc gaccatcccg tcctggatgg gcagctacta
      241 cgtcaacatc gcgagccagg tcctgatctg ggcgatcctg gcgctcggcc tcaacgttct
      301 tgtcggctat gccggcctcg tgtcgctcgg ccatgcaagc ctcttcgggg ttgccgctta
      361 cgccgtcgcc tggctggtcg cggccggctg gggccatcct ggtgcagcgc tcatggcctt
      421 cggcgtgacg gtcgtgacct cggcgatgtt cgcggtcctg gcgctgcgcg ccaccggtat
      481 cggtttcctc atgatcacgc tggcgatcgg ccagatcctg tggggaattg cctatcgctg
      541 gatcagcttc accaacggcg acaacggcat caacgtcgcg tcgcgtcccg ttcttctcgg
      601 ctggccgctg ggctcgccga tacacttcta ttatttcacg ctggccgtgt ttctgttcgc
      661 gctcgcagcg atcgccgtct tcgtcgcctc gcccttgggc gcgagcctca agggcgcccg
      721 cgaccagccg cgtcgcatga tcgcgctcgg cttcaatgtc tggctcatcc gcttcatcgc
      781 cgtgatcttc tcaggcgtct ggagcggcat cgccggcatc ctcttctgct attacaacca
      841 gttcgtgagc ccgaacgtgc tcgcgctggg aagctcagcc gaagcgctgc tcatggtgat
      901 ctccggcggc agcggcacgc ttttgggtcc cgtggccggt gccgcgctgg tggtcgtcat
      961 gaaaaacgtg gcaagcgcct atatcgagcg ctggaacctc gtgctcggcg cgatcttcgt
     1021 cgcgatcgtg ctgttcatgc cggagggtct ggtgcccggc tcggcgcggc tctggcggca
     1081 gggcagggcg gcgctgcgca caaggcggcc cgcggcgccc atcgggagcg cgccatgacg
     1141 gcgctccgtc tgcgcggcct caccaaattc ttcggcggct tgcgcgtcgc cggtggcgtc
     1201 gacttgcagg tggagcccgg cgagcgccgg ctgatcatcg ggcccaacgg cgcgggcaag
     1261 acgacgctct tcaatctcat cgccggcgag ctcaaggccg atgccggctc gatcgtgctc
     1321 ttcggccgcg acatcacgcg cgtgccgagc cgcggtcgcg cgcatctcgg cctggcgcgc
     1381 acctatcaga tcatcactct ctttccgcgc gagacgcttc tcaggaatgt cgagttgtcg
     1441 ctgctggcgc tctcgccctt gcgctggaac ccgctcgtgc cgctggcgcg ccaggcggga
     1501 ctcgcggccc gcgcccgcga agcgctcgcg cgaatcggcc tcgaggcgct ggccgagcgc
     1561 cggctcgccg acacttctta cggcgagcgg cggcgcgtcg agatcgccat ggcgttggcg
     1621 cagcggccgc gcctgctgct gctcgacgag cctttcgccg gtctctcgat cgacgaacgc
     1681 aaggacgttc aaaagctcct tctcgccatt ccgcgcgacg tcacgattgt catgatagag
     1741 catgacatgg acgtggcgct cgagctcgcg gaacgcatca cgctcatgca tttcggcgaa
     1801 gtgatcgtcg agggcacgcg ggccgaggtc gtcgcgcatc cccggacgcg ggagatctat
     1861 cttggcgcct gacgcgctga tgctcggcga catcgacgcc tactacggcg acagccatgt
     1921 gctgcatcaa gtatcgctgc ggctgcgcga aggccggctg ctcgctctgc tcgggcgcaa
     1981 cggcgccggc aagtcgacct gtatgaatgt cgcagtcggt ttgctgccgc cgcgacgcgg
     2041 cgcgatcgag ctcttcggaa cgctcatgac ggcacgctcg cccgaggtga tcgcggcaca
     2101 aggcgtggcc ctcgtgcccc agggccgccg catcttccgc agcctaacgg tgcgcgagaa
     2161 tttgacggtc gcggcgcgca agccgcgcgc cggtgcccgt catccctggg cactcgatac
     2221 cgtcttcgcc accttcccac gacttgccga gcggcgcgcg caggtggcgg gctttctctc
     2281 cggcggcgaa cagcagatgc tcgccatcgg ccgcgcgctc atgggcaatc cgcgcgttct
     2341 gttgatggac gagccgtcgg agggcctggc gccgcagatc gtggccgagg tcgtcgcgac
     2401 gatccgcaag ttgaaggagc agggcctgtc gattctcctc gtcgagcaga acgcgaaact
     2461 ggccttcgat ctcgcggacg acgtcgtcat tttgaacagc gggcgcgtcg tgctcgacct
     2521 tccggtggag cagctgaagc gcgacggcag cgatttgcac cagcatctcg gcgtgtatta
     2581 gaaccgcggc gcgacaggga gacccatgac gtccggcaga cacgaggcga aatggcgcgg
     2641 gcgcgtggaa gacgagtcgc tgctgcgcgg cgccgggcgc tatgccgccg acgcgcacat
     2701 tcccggtcag gcgacggcgt ggttcgtgcg ttcgccacac gccttcgcca gcattcgcaa
     2761 ggtcgacacg cgcgcggccg aggcggcgcc gggcgtgctc gccgtgctga ccgccggcga
     2821 catggcggcc accggcgtcg gcaatctctc gcgtccggtg ccgcaaagcg gtcgtgatgg
     2881 cgctccgttg cgtgggtcga tgcgcctcgc gctggcggag gaccgcgtcc gccatgtcgg
     2941 cgagccggtg gcgctcgtcg tcgccgagac gcgcgcggcg gcacaggatg ccgccgatct
     3001 ggtcgaggtc gagtacgatc ctctgacgcc ggtcgtggag ctgcgccggg ccgttgcttt
     3061 gggcgcgcct cagctccatc gcgaagcgcc gggcaacgtc gcgctcgact ggggcttggg
     3121 tgcgacgccg gagaatctcg cggccatcga aagcgtgttc ggcgcggcgg cgcatgtgtc
     3181 acgcatctcc cttgtcaatc agcccatcgc cgtcgcttcc ctcgagccgc gcggcgcaac
     3241 ggcgatctat gacccggcaa gtggcggcta cacgctgcgc tgcggctcgc agggtgtcat
     3301 gggcctgcgc gaccagctcg tcgctattct cggcatcgag caagaccgct tgcgcctcat
     3361 caccgaggac gtgggcggcg gcttcgggat gaaatcggga gcctatcccg aatatccagc
     3421 gcttttggtt gccgcgaagc ggatcggccg tcccgtgcat tggatgtcga cgcgcgccga
     3481 ggctttcgtc accgatcagc tggcgcggga tacaatcaca gaggcggcgc tcgcgctcga
     3541 cgcgcagggg cgctttctcg ctcttcgggt gaaagtgctc gcgagcatgg gcgctcatgt
     3601 gacagcggtg ggtgccttca ttgccaccag caatttcgcc cgctgcctca gcagcgtcta
     3661 ccgcatcccc cagatcgctg ccgaaatccg ctgcgtctac accaacactg tgccgaccgg
     3721 cccctatcgt ggcgcgggcc gccccgaagc caattacgcg atcgagcgcg tcatcgacga
     3781 ggccgcgcgg cgcaccggca tcgaccgcat cgatctgcgc cggcgcaatc tcgtgacgcc
     3841 ggccgagatg ccctacaaga cgccgatcgg tgtcacctac gacagcggcg actttcccgc
     3901 catcttcgag gaagcgctac agcgcgccga tgtcgcgggc ttcgccgcgc ggcgcaagcg
     3961 tgaagcggcc gccggcaagc gctgcgggct cggcatctcc tgctttctcg agcatgcggg
     4021 cggcccgccc gtcgagggcg ccgcgctggc ctttccgggc ggccgcagcg tcgcgctcga
     4081 tctcgccacc ggagccaccg gccagggcca cgctacggtc tttcgccgcc tcgtcgccga
     4141 gcgcctcggc ctcgccgagg cgcaagtgac gatgcgcgcc ggcgacactg cgctcggcgt
     4201 ccagggcagc ggcaccatcg cctcgcgcaa caccatggcc acgggcacgg cttccctgcg
     4261 tgccgtcgag gcggttatcg agaagggcct gcaccaggcg gcgcgtcttc tcgaagcggc
     4321 cgaggccgat atcgactatg gcgcaggcag cttccgcatc aggggcaccg accgctccgt
     4381 gtcgctcttc gacgtggccg agcaggcgaa ggccgccggc gagccgctcg acagcaatgt
     4441 gcgcgtcgaa gtgccgcaat ccttccccaa tgggtgccat atcgccgaga tcgagatcga
     4501 tcccgagacc ggcgtgctcg agatcgtcgc ctatgtcgcg gtcgacgatt gcggcgtggc
     4561 gctcgatccg gtgctcgtcg aggggcaaat ccaggggggc gtcgcgcaag ggatcggcca
     4621 ggtgctgatg gagcacgccg tctatgacgg cgcgaccggc cagctcctgg ccggctcctt
     4681 catggactat gccatgccgc gagccgatac cgtgcccgac gtccagggca cgctctaccc
     4741 ggtgccgtgc cggaccaatc cgctgggcgt gaagggcgcc ggcgaggcag ggacgaccgc
     4801 agcgctcgcc gccgtcatga atgcgatcgc cgatgccgtc cccggcatcg ccatcgacat
     4861 gccggcgacc gccgagaagc tgtggcgcgc ctgccgcaat ctctgaacag aaaggaagtt
     4921 tgccgatgct ccatcgatgc ggcccagcga tgcctcatgc catgagggcc gacgccaagc
     4981 gcgcgcggcg cggcgcggcg ccgcgccggc atttcaccgt cgacatccac tgccatgtct
     5041 tcacgcccga agccgagcgc atcgtgcaat ccggctttca tccggagatg gagccgatgt
     5101 tccgctatgc caacgaggcg acgcgcgacg tcaatcgccg gcagacggag gcgatccgcg
     5161 gcaagctcac ctcgatcgag acgaggctcg gcgacatgga ccgtctcggc atcgatgtcc
     5221 aggcgatctc gccttcgccg ccgcaatact attattgggc ggcgcccgag ctcgggcgcg
     5281 aggcggcgcg tctcgtcaac aatcgcatcg ccgagatcgt ggc
//
LOCUS       DASYNS010000391         4714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5245268, whole genome shotgun sequence.
ACCESSION   DASYNS010000391 DASYNS010000000
VERSION     DASYNS010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5245268"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1179
                     /locus_tag="VGP50_12210"
     CDS             28..1179
                     /locus_tag="VGP50_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HEV8028188.1"
                     /translation="MTSTLSKSDVARLLTDSSPETRADVAAKVAREVDGSALTPEERA
                     IAEDILRALSRDAATRVRQALAEELKHSPRLPRDVALALAQDAAELVALPVLAGSAVL
                     TDEDLIAMVRAGSATRQAAIAARRHVSADLAAAILEADQPQALAALVGNDGAELAEPM
                     LQRVLDRHGDDDTVKTPLARRRTLPITILERLVAAASAQLRTVLAERGDLPEHLASDL
                     VLGTRERATASLLSADSLASQALTLAKHLHGNGRLTAPLIVRAICLGDLPFVEAAFAV
                     LADIPLHNARLLIYDAGQLGFKALYERCKLPADFFNLFRVALRVAQQTEFDGGDDDRE
                     RRRRRTLERILTQYENIGSEDLEYLLSRLRAEAAAESAERPRSVAPARS"
     gene            complement(1337..3034)
                     /locus_tag="VGP50_12215"
     CDS             complement(1337..3034)
                     /locus_tag="VGP50_12215"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028189.1"
                     /translation="MPHPRDWFRRRPAVAADGIRPQAAGDAVLDLGQNGTRLREPPPL
                     HDHRPLPLAERRGPVPPPRAPLQRREGVTLDRSAFTVRPELVRARPAETANGASLATF
                     AVRGAAAAQRAVAAAAPQEAPVPETKARIEPLPLPAEAKPRVVDGTTAESAGDAQFIL
                     GTPGGAWKRGRSPLGEAPSAGAQLREAFTPTRPKQDATLFSGRFKQMQRIIAAIEEER
                     AHVVIYGERGSGKTSLANVLAAKSAEAGYFVLRFACSSDLSFADIFRSFLRRMPATLL
                     ADGIGALSRARVENFEQLVPAQFGIPELIAALERVHDRHVILVVDEYDRVTAEETKAK
                     FAELIKNMSDATAPVTLVIIGVADDVRQLLGKHPSLQRTLVTVPMPLMTRREIDGIIA
                     IGEEKSGLRFDPWVRQTIVDFAQGLPYHAQLLSLFAARHALRRQSTRVEREDLRYAVA
                     RVAEEAEPRLKDAYDLAIGPHENASFRDVLFHAARCRTDEFGVFSAADVAAAAAGADS
                     AGVSLLALQYPLKKLTEPERGAVLRRIVAPGGLRYQFVTLMMRHHVLMRQAEQRQLV"
     gene            complement(3181..3558)
                     /locus_tag="VGP50_12220"
     CDS             complement(3181..3558)
                     /locus_tag="VGP50_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028190.1"
                     /translation="MAAAPSRSAVTTLRLPHYLLLIIAAWMVAMTWRVYPQFKDMLKV
                     EDRLVTLDDYVEESCGQRIGPAAASCVEEARRTGRRLVARQQGRSLLFVVAPFLAYLA
                     IHLPAQSMAARRARSHRPAASGE"
     gene            complement(3823..4455)
                     /locus_tag="VGP50_12225"
     CDS             complement(3823..4455)
                     /locus_tag="VGP50_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028191.1"
                     /translation="MWLKSLSVGVLAALIAIPAFAQAPEGTLVRIRGKVEKLDGSTLT
                     VKGREGKNPASDVSVMLAPDVVILAVKKIQLSDIKQGDFIGSAAVPGKDGKLKAEEVL
                     VFPEMARGTGEGHYPWDRDLKSSDETMTNATVSGVASAAKGEVLMVQYKGGEAELVVG
                     PKVPVVTFAPDDKALLKSGATVFIPALKKPDGTIVANRVIAEKDHVKPPM"
     gene            4618..>4714
                     /locus_tag="VGP50_12230"
     CDS             4618..>4714
                     /locus_tag="VGP50_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV8028192.1"
                     /translation="MESLAAEIARRRTFAIISHPDAGKTTLTEKLL"
BASE COUNT          752 a         1615 c         1590 g          757 t
ORIGIN      
        1 gatgctccgc gaggtcgccc gccaaagatg accagtaccc tctcgaaatc cgacgttgcc
       61 cggctgctca cggattcttc gcccgagaca agggcggatg tggcggcaaa ggtggcgcgc
      121 gaggtggatg gaagcgcgct cacccctgaa gagcgtgcga tcgccgagga cattctccgc
      181 gcgctcagcc gcgatgcggc gacgcgcgtg cgccaggcgc tcgccgaaga gctcaagcac
      241 agtccgcgcc tgccgcgcga cgtggcgctg gcacttgctc aggacgccgc cgagctcgtc
      301 gccctgccgg tgctcgcggg ctctgcggtg ctgacggatg aagatctcat cgccatggtg
      361 cgtgccggca gcgccacgcg ccaggcggcg atcgccgcgc ggcgccacgt ctccgccgat
      421 ctcgcggcgg cgatcctcga ggccgatcag ccgcaggccc tggcggcact cgtcggcaat
      481 gacggcgccg agctggccga gccgatgctg cagcgtgtgc tcgaccggca tggcgacgac
      541 gacaccgtga agacgccgct ggcgcggcgg cgaacgctgc cgatcaccat cctcgagcgc
      601 ctggtggccg cggcatcggc gcagctccgc accgtgctgg ccgagcgcgg cgatctgccc
      661 gagcatctcg cgagcgacct cgtgctcggc acgcgcgagc gtgccaccgc gagccttctc
      721 tcggctgact cgctcgcttc ccaggcgctc acgctggcga agcatctgca cggcaacggc
      781 cggctgacgg cgccgctcat cgtgcgcgcc atctgcctcg gcgatctccc tttcgtcgaa
      841 gcggcctttg ccgtgctcgc cgacatcccg ctgcacaatg cgcggctgct gatctacgac
      901 gcaggacagc tcggcttcaa ggcgctctat gagcgctgca agctgccggc ggatttcttc
      961 aacctgttcc gcgtcgccct gcgggtcgcg cagcagaccg aattcgacgg cggcgacgac
     1021 gaccgcgagc gccggcgccg gcgcacgctc gagcgtattc tgacgcaata cgagaacatc
     1081 ggatccgagg atctggaata tctgttgagc cggctgcgcg ccgaggccgc cgcggagtcg
     1141 gcggagcggc cgcgctccgt cgcgccggcc cgttcctaaa gcagatctcg agcaggtgga
     1201 atcgacactt cccaacatcg tcattcccgc gcaagcggga atccagggct gccagcacgt
     1261 cgctcgcccc tggcgtcccc gctttcgcgg ggaagacgaa gaaagaacgg ttccaggtaa
     1321 acgggatgcg ctctaactac accagttggc gctgctccgc ctggcgcatc agcacgtggt
     1381 ggcgcatcat cagcgtcacg aactggtagc ggagaccgcc cggggcgacg atgcgccgca
     1441 acacggcgcc gcgctcgggc tcggtgagct tcttcagcgg gtattgcagg gcgagcagcg
     1501 acacgccggc agaatcggcg ccggctgccg cagcggccac gtcggcggcc gaaaagacgc
     1561 cgaattcgtc cgtccggcac cgcgcagcgt ggaacagcac gtcgcggaag gacgcattct
     1621 catggggacc gatggcgagg tcgtaagcgt ccttgaggcg cggctcggcc tcctcggcga
     1681 cgcgcgcgac ggcgtagcgc aaatcctcgc gctcgacgcg ggtcgactgg cggcggagcg
     1741 catggcgcgc ggcaaacagg ctcagaagct gagcgtggta gggcaggccc tgggcgaaat
     1801 cgacgatcgt ctggcgcacc cagggatcga agcgcagccc ggatttttcc tcgccaatgg
     1861 cgatgatgcc gtcgatctcg cgccgcgtca tcagcggcat cggcacggtg acgagcgtgc
     1921 gctgcaagga ggggtgcttg ccgagaagct ggcggacatc gtcggcgacg ccgatgatca
     1981 ccagcgtcac cggcgccgtg gcgtccgaca tgttcttgat gagctcggcg aacttcgcct
     2041 tggtctcctc ggccgtcacg cggtcgtatt cgtcgacgac gaggatgacg tgccggtcgt
     2101 gaacgcgctc caaggcggcg atgagctcgg ggatgccgaa ctgcgcgggc accagctgct
     2161 cgaaattctc gacgcgggca cggctcaagg caccgatgcc gtcggccagc aacgtcgcgg
     2221 gcatgcgccg caggaaactg cggaagatgt cggcgaagct cagatccgaa ctgcaggcga
     2281 aacgcaggac gaaatatccc gcttccgccg atttggcggc gagcacattg gccagcgagg
     2341 tcttgccgct gccgcgctcg ccatagatga ccacatgggc gcgctcttcc tcgatggcgg
     2401 cgatgatccg ctgcatctgc ttgaagcgcc cggagaagag cgtcgcgtcc tgcttcggcc
     2461 gcgtcggcgt gaaagcctcg cggagctggg cgccggcgct tggggcctcg ccgagcgggg
     2521 agcggccgcg cttccaggcg ccacccggag tgccgaggat gaattgcgca tcgcccgcgc
     2581 tttcggcggt cgtgccgtcg acgacgcgcg gcttcgcctc ggcgggcaaa ggaagcggct
     2641 cgatccgggc tttcgtctcg gggaccgggg cttcctgggg cgccgcagcc gcgacggcgc
     2701 gctgggccgc agccgcgccc ctcaccgcga aggtcgcgag cgaagcgccg ttcgcggtct
     2761 ccgccgggcg tgcgcggacg agctccggcc gtaccgtgaa ggcgctgcgg tcgagcgtca
     2821 cgccttcgcg gcgctggagc ggtgcgcgcg gcggcggcac cggaccgcgg cgctcggcga
     2881 gcggcagcgg cctatggtcg tgcaagggtg gcggctcgcg cagtcgcgtg ccgttctggc
     2941 cgagatccag cacagcgtcg cctgccgctt gcgggcgaat gccatcggcc gcgacagcgg
     3001 gacggcggcg aaaccagtct ctgggatggg gcatggactc tacccatggc ttttggggcg
     3061 caaccggcgc ccgactatga cgcgaatgcg cagccggctc cgtctacgct gtggtttaac
     3121 ggcacgccac ggcaaaggtt tcaccgggcc ccaaaaaagc ccttctgtgt ggtacccgcg
     3181 tcattcgccc gaggcggcgg ggcggtgcga gcgcgcgcgg cgcgcggcca tgctctgcgc
     3241 gggcagatgg atcgcgagat aggcgaggaa cggcgccacg acgaaaagga gggagcgtcc
     3301 ctgctgccgc gccaccaggc gacggccggt gcgacgcgcc tcctcgacac agctcgcggc
     3361 ggccgggccg atccgctgcc cgcaactctc ctcgacatag tcgtccaagg tgacgagacg
     3421 atcctcgacc ttgagcatgt ccttgaactg aggatagacg cgccatgtca tcgcgaccat
     3481 ccacgcggcg atgatgagga ggaggtaatg cggcagccgc agcgtcgtca cggcagagcg
     3541 ggaaggagcg gccgccacgt cgcgtcgttc cggccatggc gcgccaaccc cgccacgtct
     3601 gcaccgatgg cggggttcaa accgggtggc tccgcgagct caccgctcgg tcacgcgacc
     3661 gagccttcat ccgggcgctg tctaacaatg atccggtgga gacatgcttg ttaagcaccc
     3721 tccgtctctc gttgcgccac tctctcattt gtgtcgccac aaaaggtcga tcaagggtgg
     3781 cgcgcggtaa tcttgttgcg ttttcgcgcg ccgcgccccg catcacattg gcggcttcac
     3841 atgatctttc tctgcgatca cgcgattggc gacgatcgtg ccgtccggct tcttgagggc
     3901 cgggatgaac accgtcgcgc cgctcttgag caacgccttg tcgtccggcg cgaacgtgac
     3961 gaccggcacc ttcggaccga cgacgagctc tgcctcgcca cccttgtatt gaaccatcag
     4021 gacttcgccc ttcgccgcac tggcgacgcc ggaaacggtg gcgttggtca tcgtctcgtc
     4081 actgcttttc aagtcccgat cccaggggta atgcccttca cccgtgccgc gcgccatctc
     4141 ggggaagacc agaacctcct cggccttgag cttgccgtct tttccgggca ccgccgccga
     4201 accgatgaag tcaccctgct tgatgtccga cagctgaatc ttcttcacgg cgagaatgac
     4261 gacatccggc gcgagcatca cggagacgtc gctcgccggg ttcttccctt cgcggccctt
     4321 caccgtcagg gtcgagccgt cgagcttctc cacctttccg cgaatgcgca cgagcgtgcc
     4381 ctcgggcgct tgggcgaagg ccgggatcgc gatcagggcg gcaagcaccc cgacgctaag
     4441 cgatttgagc cacatgagtt tttgtactcc cccaccgttt tgcagcgcag gatgcgcgtc
     4501 gcgcttcgta ccatagcgct cggaaggccg cctaagcgcc gggcttcaca ttttcgtgac
     4561 cttcgggacg ctccggccgt tgctttcccg ggcgccctcg attagagacg cggtgccatg
     4621 gagtcgctcg ccgccgaaat cgccagacgg cgcacctttg ccatcatctc ccatcccgac
     4681 gccggcaaga cgacgctcac cgagaagctg ctgc
//
LOCUS       DASYNS010000392         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2126967, whole genome shotgun sequence.
ACCESSION   DASYNS010000392 DASYNS010000000
VERSION     DASYNS010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2126967"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VGP50_12235"
     CDS             <1..879
                     /locus_tag="VGP50_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV8028193.1"
                     /translation="LLTGQLPNRAVPGTRWQGERYDGKRLLIVSEQGFGDAIWIARYL
                     PRVKALGGELIFECRPEAIPLIAAMNVVDRIVPKGDPLPDADLHLYQCSLPGLYTPTL
                     AAIPRAPYLPVETHRLGKFAAAMAKGQGKLKVGIVWSGSTTFGANHDRAVQLRMFLQW
                     FALPGVQLYSLQKGPPEAELTALPAGAPIIDLAPLLGDFADTAAAIAQLDLVLMTDSA
                     VAHLAGALGKPVWVLLSFVPHWLWLLERAESPWYPALRLFRQRAWGDWTGVFDQAAAA
                     LLDLATTKTHPRSTAR"
     gene            1100..1174
                     /locus_tag="VGP50_12240"
     tRNA            1100..1174
                     /locus_tag="VGP50_12240"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1132..1134,aa:Val,seq:cac)
     gene            1388..1705
                     /gene="groES"
                     /locus_tag="VGP50_12245"
     CDS             1388..1705
                     /gene="groES"
                     /locus_tag="VGP50_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV8028194.1"
                     /translation="MQFRPLHDRVVVRRLEQEEKTAGGIIIPDTAKEKPQEGEVIAVG
                     PGARGEDGTLHPLDVKTGDRVLFGKWSGTEVKIDGEDLMIMKESDIMGIIEGKPKAAK
                     KVA"
     gene            1752..3389
                     /gene="groL"
                     /locus_tag="VGP50_12250"
     CDS             1752..3389
                     /gene="groL"
                     /locus_tag="VGP50_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011387923.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV8028195.1"
                     /translation="MAAKDVKFHGDARDRLLRGVDILANAVKVTLGPKGRNVVLDKSF
                     GAPRITKDGVTVAKEIELADKFENMGAQMLREVAQKTNDIAGDGTTTATVLAQAIVRE
                     GVKAVAAGLNPMDLKRGIDLAVEAVVEDVKKRSKKVSTNDEIAQVGTIAANGEADIGQ
                     KIAEAMQKVGNEGVITVEEAKSLETEIDTVEGMQFDRGYLSPYFVTNAEKMLCELENP
                     FILLHEKKLSGLQAMLPVLEAVVQSGRPLLIVAEDVEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAILTGGQLISEDLGIKLENVTLDMLGKAKKVRVEKENTTIID
                     GSGKKKDIEGRIAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKER
                     KDRVDDAMHATRAAVEEGIVPGGGVALLLATKALGKLKPENDDQKVGIDIVRRALQAP
                     VRQIAENAGVDGSIVVGKLLESTTANYGFDAQTGQYVDMLKAGIIDPTKVVRTALQDA
                     ASVAGLLVTTEAMVAEKPEKKAPAMPPGGGGMGDMDF"
BASE COUNT          647 a         1115 c         1136 g          522 t
ORIGIN      
        1 ctgctgacgg gtcagctgcc caaccgcgcg gtccccggca cgcgctggca gggcgagcgc
       61 tatgacggca agcgactcct catcgtcagc gagcagggct tcggcgatgc catctggatc
      121 gcgcgctatc tgccgcgcgt caaggcgctg ggcggcgagc tgatcttcga gtgccggccc
      181 gaggcgatcc cgctcatcgc cgcgatgaat gtggtggacc gcatcgtccc caagggggat
      241 ccgctgcccg acgccgatct tcatctctat caatgcagcc tgccggggct ctacacgccg
      301 acgctcgccg cgattccgcg cgcgccctat cttccggtgg aaactcaccg gctcggcaaa
      361 ttcgcggccg ccatggccaa aggacaaggc aagctgaagg tcggcatcgt ctggtcgggc
      421 agcaccacct tcggcgccaa tcacgaccgc gccgtgcagc tgcgcatgtt cctgcaatgg
      481 ttcgcgctgc cgggcgtgca gctctacagc ttgcagaagg gtccgccgga agcggagctc
      541 acggctttgc ctgcgggtgc gccgatcatc gatttggcgc cgctgctcgg cgatttcgcg
      601 gacaccgcgg ccgccatcgc gcagctcgac ctcgtgctca tgaccgacag cgcggtggcg
      661 catctcgcgg gcgcgctggg caagcccgtg tgggtgctgc tgagcttcgt gccgcactgg
      721 ctctggctgc tcgagcgcgc ggagagcccg tggtatcccg cgctgcggct cttccgccag
      781 cgcgcctggg gcgactggac cggcgtcttc gaccaggccg ccgcggcgct gctcgacctc
      841 gcgacgacga agactcaccc tcgatcgaca gctcgctgag tcgccatcat cggcgacggg
      901 aagccccatc gcgtggccga ggcagccgcg gcgccccgga gcttgagtac gtctcgacgc
      961 agcgcatcgc cgcctcgccc gctgcgatcg ccggcgcctt gccaagccgc aggcgattcg
     1021 ttatctcctg tcggcgtttc tgctcggtcg ggtcgccgtc gtcgaccact gccaggatca
     1081 ctcgacttac gcgggatacg ggcgcttagc tcagcgggag agcacaccct tcacacgggt
     1141 ggggtcgtag gttcaatccc tacagcgccc accatcccgc agaagctaac gtgagacccc
     1201 ttcggccgcg cgcagaaaat ccctgagatc tcagcggctt gcgaaaattc ttaccaattc
     1261 ctctacggcc ctcttgaaag cggcaaaggc ctcgactaac tttctggcac tcacggccgg
     1321 agagtgctaa cagctctgtc cttgtgtccc atagccaatt cgaaatttcg ctgaggagga
     1381 ggattcgatg cagttccgtc ccttgcatga ccgcgtggtg gtgcggcgcc tcgagcaaga
     1441 agagaagacc gccggcggca tcatcatccc cgacaccgcc aaggagaagc cgcaggaagg
     1501 cgaagtgatc gccgtcggcc ccggcgctcg gggcgaggat ggcacgctcc atccgctcga
     1561 cgtcaagacc ggcgaccgcg tgctgttcgg caagtggtcc ggcaccgagg tcaagatcga
     1621 cggcgaggac ctgatgatca tgaaggaaag cgacatcatg ggcatcatcg aaggcaagcc
     1681 caaggcggcc aagaaggtcg cctgatcgct caccccattc ccacgcacga aacttcagag
     1741 agtagacaaa catggctgca aaagacgtca agtttcatgg cgatgcgcgc gaccggctgt
     1801 tgcgcggcgt cgacattctg gcgaacgcgg tgaaagtgac gctcggtccc aagggccgca
     1861 acgtcgtgct cgacaagtcg ttcggcgcac cccgcatcac caaggacggc gtgaccgtcg
     1921 ccaaggagat cgagctcgcc gacaagttcg agaatatggg cgcgcagatg ctgcgcgagg
     1981 tcgcccagaa gaccaacgac atcgccggcg acggcaccac caccgcgacg gtgctggccc
     2041 aggcgatcgt ccgcgagggc gtcaaggcgg tggccgccgg cttgaacccg atggatctga
     2101 agcgcggcat cgacctcgcc gtcgaggcgg tggtcgagga cgtgaagaag cgctcgaaga
     2161 aggtctcgac caacgacgag atcgctcagg tcggcaccat cgccgccaat ggcgaagccg
     2221 atatcggcca gaagatcgcc gaggccatgc agaaggtcgg caacgagggc gtgatcacgg
     2281 tcgaggaggc gaagagcctc gagaccgaga tcgacaccgt cgaaggcatg caattcgacc
     2341 gtggctatct ctcgccctat ttcgtaacga acgccgagaa gatgctgtgc gagctggaga
     2401 atccgttcat cctgctccac gagaagaagc tgtccgggct gcaggcgatg ctgccggtgc
     2461 tcgaggcggt ggtgcagtcg ggccggccgc tcctcatcgt cgccgaggac gtcgagggcg
     2521 aggcgctcgc gacgctcgtc gtcaacaagc tgcgcggcgg cctcaaagtg gctgcggtga
     2581 aggcgccggg cttcggcgat cgccgcaagg cgatgctcga ggacatcgcc atcctcacgg
     2641 gcggccagct catcagcgaa gatctcggga tcaagctcga gaacgtcacg ctcgacatgc
     2701 tgggcaaggc caagaaggtg cgcgtcgaga aggagaacac gacgatcatc gacggctccg
     2761 gcaagaagaa ggacatcgag ggccgcatcg cccagatcaa ggcgcagatc gaggagacca
     2821 cctccgacta tgaccgcgag aagctgcagg agcggcttgc caagctcgcg ggcggcgtcg
     2881 cggtgatccg cgtcggcggt gcgaccgagg tcgaggtgaa ggagcgcaag gaccgcgttg
     2941 acgacgccat gcacgcgacc cgcgccgcgg tcgaggaagg catcgtcccc ggcggcggcg
     3001 tggcgctctt gctcgccacc aaggcgctcg gcaagctcaa gcccgagaat gacgaccaga
     3061 aggtcggcat cgacatcgtc cgccgcgcgc tccaggcgcc ggtgcggcag atcgccgaga
     3121 atgccggcgt cgacggctcg atcgtcgtcg gcaagctgct cgagtcgacc acggcgaatt
     3181 acggcttcga tgcgcagacc ggccagtatg tcgacatgct gaaggccggc atcatcgacc
     3241 cgaccaaggt ggttcgcacc gcgctccagg atgcggcctc ggtcgccggc ctcctcgtca
     3301 ccaccgaggc gatggtcgcc gagaagccgg agaagaaggc gccggcaatg ccgccgggcg
     3361 gtggcggcat gggtgacatg gacttctgat tgacgctcat cgctgcgaaa aaggaggggc
//
LOCUS       DASYNS010000393         4350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_566324, whole genome shotgun sequence.
ACCESSION   DASYNS010000393 DASYNS010000000
VERSION     DASYNS010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566324"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1156)
                     /locus_tag="VGP50_12255"
     CDS             complement(50..1156)
                     /locus_tag="VGP50_12255"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV8028196.1"
                     /translation="MDSIALERATVAPPATRPDLRRRWRRLGLAAALFAAATGLAWYA
                     QDWWRVGRFIESTDDAYVGGNVTPLAPHIDAFIEQVLVTDNERVAAGQLLIRLDQRDY
                     RAALDHAEASLAARLAAAESLRAQSALQQSTIRQQEADLVAKNAGAAFAAEDASRYRF
                     LAQATVGSRQNEQRSAAADQEARAAVVAGRAGLEAAQQQIKVLDAQIAEADAAVAQAN
                     SELRTAELNLGYSEIRAPTDGYVGNRAAQIGAYATRGTYLISVTPADGLWVDANFKED
                     QLARMLPGQSASVTADALPGHVFRGHVASFAPGTGAVFSVIPPENATGNFTKIVQRVA
                     VRIALDGGDPMLATLRPGLSTIVRVETRGEKGAP"
     gene            complement(1184..1648)
                     /locus_tag="VGP50_12260"
     CDS             complement(1184..1648)
                     /locus_tag="VGP50_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEV8028197.1"
                     /translation="MAPESWSTPDVCNCLALRQAARHVTQFYDQFLAGAGLRTTQFSI
                     LAKLRRLGPLSINALAKELVMDRTTLGRNMLPLEREGLIAIVRARGDRRSKELQLTDA
                     GLARLRQALKGWTTAQARFETVFGDKRTAELRSLLHAVSASDLGGAADDAAD"
     gene            1868..2080
                     /locus_tag="VGP50_12265"
     CDS             1868..2080
                     /locus_tag="VGP50_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028198.1"
                     /translation="MATFLSVGWAAVYCTTFGMWCSILAMNRGRDWLIWLLLGASCGL
                     FALIILLCLPNLQTKEDQLHPLVDVD"
     gene            complement(2077..3042)
                     /gene="glk"
                     /locus_tag="VGP50_12270"
     CDS             complement(2077..3042)
                     /gene="glk"
                     /locus_tag="VGP50_12270"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEV8028199.1"
                     /translation="MRRLIGDIGATNARFAWADADGRTDRIRVLACDDYPTIEDALAA
                     YLADDDRPEVPREVALAVASPITGNAVAFTNHPWSFTISGLKRRFALERLLVVNDFTA
                     NALAMPRLSPSERIAIGGGAPVAEAAIGVLGPGSGLGVSGLVWTPGGWVPLSGEGGHA
                     TMAACDARETAVLERMRKRFDHVSAERVLSGPGLVNLYNILAELEGVPAAPFTPAQIA
                     DRHLGEAEPIARAALAMFCAMLGSFAGNLALTLGARGGIYIAGGIVPRLVPSLLDSAF
                     RMRFEAKGRFQPYLATIPTYVVAHPIPAFLGLVALLDEKPARSGV"
     gene            complement(3058..>4350)
                     /locus_tag="VGP50_12275"
     CDS             complement(3058..>4350)
                     /locus_tag="VGP50_12275"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEV8028200.1"
                     /translation="ALRSSGGIWFGWSGEVTATPATAPVLVEGDGFVYATLDLARQDY
                     EQYYNGFANRVLWPLFHYRPGFVDYQRADFAGYMRVNRQFAAQLAPLLRDDDFIWVHD
                     YHLIPLGDELRRLGVKQPIGFFLHTPFPAAEVFRILPTHAKIIRALCAYDLVGFQTED
                     DLSAFRDYLLRWSGAELGRQGEISAFGRRLRAAAFPIGIDVQAIATQAAQSVGARHMR
                     RLHDSLRDRSLMIGVDRLDYSKGLEARFKAFQRLLEMFPETRGRVTLMQIAPPTRSEV
                     PEYLEIRRNLETAAGHINGRFAEFDWMPLRYLNKSFNQRALCGFLRAARIGLVTPIRD
                     GMNLVAKEYVAAQDGGDPGVLVLSCFAGAARELRDALLVNPFDIDGMAEALHEALAMP
                     LGERIERWQSMMGTLKRNDITAWRENFVEALARTEAVH"
BASE COUNT          710 a         1418 c         1545 g          677 t
ORIGIN      
        1 acgcacatga cgacgaaggg caggagcgag ggatgcttcg gttcggcggt catggcgcgc
       61 ctttctcgcc gcgggtctcg acgcgcacga tggtcgagag gccgggcctg agcgtcgcga
      121 gcatcgggtc gccaccatcg agcgcgatgc gcacggccac gcgctgcacg atcttggtga
      181 agttgccggt ggcgttctcc ggcggaatca cgctgaagac ggcgccggtg ccgggcgcga
      241 agctcgccac atggccgcga aagacgtgac cgggcagcgc atcggccgtc acgctggcgg
      301 attggcccgg taacatccgg gcgagctgat cctccttgaa attggcgtcg acccagagcc
      361 cgtcggcggg cgtgacggaa atcaaatagg tgccgcgcgt ggcataagcg ccgatctgag
      421 cggcgcggtt gccgacatag ccgtcggtcg gcgcgcgaat ctcgctgtag ccgagattga
      481 gctccgcggt gcgcagctcg gagttcgctt gcgccaccgc cgcgtccgcc tcggcgatct
      541 gggcgtcgag caccttgatc tgctgctgcg cggcctcgag cccggcgcgg cccgcgacca
      601 cggcggctct cgcctcctgg tcggccgcgg cgctgcgctg ctcgttctgg cgcgagccga
      661 cggtcgcctg cgccaggaag cgatagcggc tcgcgtcctc ggccgcgaag gcggcgccgg
      721 cgttcttcgc gacgagatcg gcttcctgct ggcggatcgt cgattgctgc agcgccgatt
      781 gcgcgcgcag gctctccgcc gccgcgagcc gcgccgcaag cgacgcttcc gcgtggtcga
      841 gggcggcgcg gtagtcccgc tgatcaagcc ggatcagcag ctgaccggcg gccacgcgct
      901 cattgtccgt caccagcacc tgctcgatga aggcatcgat atgcggcgcg agcggggtga
      961 cgttgccacc gacataggcg tcatcggtgc tttcgatgaa gcggcccacc cgccaccaat
     1021 cctgcgcgta ccaggcgagt ccggtcgcgg ccgcgaagag cgcggccgcg aggccgagcc
     1081 gccgccagcg acggcgcaga tcgggcctgg tggcgggagg ggcaaccgtc gcgcgttcga
     1141 gcgcaatgct gtccatgagc ttcactccgc gcgaagcagg gggtcaatcg gctgcgtcat
     1201 cagcggcgcc gcccaggtcg ctcgccgaga ccgcgtgcaa gagagaacgc agctcggccg
     1261 tcctcttgtc gccgaagacg gtctcgaacc gtgcctgggc tgttgtccag cctttgagcg
     1321 cctggcgcag ccgcgccaat ccggcatcgg tcagctgcag ctccttgctg cggcgatcgc
     1381 cccgcgcgcg aacaatggca atgagcccct cgcgctccag cggcagcatg ttgcgtccga
     1441 gtgtcgtgcg atccatcacg agctccttgg cgagcgcgtt gatcgagagc ggccccagcc
     1501 gtcggagctt cgccaggatc gagaattgcg tcgtgcgcag cccggccccc gccaggaact
     1561 ggtcatagaa ttgcgtgaca tggcgcgccg cctgacgcag tgccaggcaa ttgcagacat
     1621 cgggtgtcga ccaggattcg ggtgccatcg tcgagccctc cggaaagccg aataggcgta
     1681 gtgcaccgat ataggagcat atacgcgtaa atcaagctcc ccgcaccgac ggacggtgcg
     1741 gcagcggcga tggcgaccgc catgggcgcc ttgtccgcct agaggcctcc ggaagggcga
     1801 ctatgtcgtt taaccgcgcc tttacgggct cgatgcaaga aaaggggtca gtccggagtg
     1861 tcttgtgatg gccacgttcc tatcggtcgg gtgggcagcc gtctactgca ccacattcgg
     1921 catgtggtgc agcattctcg ccatgaatcg cgggcgcgat tggctcatct ggctcctgct
     1981 cggagccagc tgtggtcttt tcgcgctgat catcctgctc tgccttccca atcttcagac
     2041 gaaagaagac cagctccacc cgctcgtcga cgtcgactag acgcccgacc tcgcgggctt
     2101 ctcgtcgagc agcgccacga ggcccaagaa ggccgggatc gggtgcgcca cgacataggt
     2161 cgggatggtg gcgaggtagg gctggaagcg gcccttggct tcgaagcgca tgcggaaggc
     2221 gctgtcgagg aggctcggca ccagccgcgg aacgataccg cccgcgatgt agatgccgcc
     2281 gcgcgcgccc agagtgaggg cgaggttgcc ggcaaagctg ccgagcatgg cgcagaacat
     2341 ggcgagcgcc gcccgcgcga tcggctccgc ctcgccgaga tggcgatcgg cgatctgcgc
     2401 cggcgtgaag ggcgcggcgg gcacgccctc gagctccgcg aggatgttgt agagattgac
     2461 gagaccgggg cccgagagca cgcgctcggc ggagacgtga tcgaaccgct tgcgcatgcg
     2521 ctcgagcacc gcggtctcgc gcgcgtcgca ggccgccatg gtggcgtggc cgccttcgcc
     2581 cgagagcggg acccagccgc ccggcgtcca gacaagcccg ctcacgccga gcccgctgcc
     2641 gggcccgagg acgccgatgg cggcttcggc gaccggtgcg ccgccgccga tggcgatgcg
     2701 ctcgctcggc gacaggcgcg gcatggccag cgcattggcg gtgaagtcgt tgacgaccag
     2761 caggcgctcg agagcgaaac gccgcttcag gccgctgatc gtgaaggacc agggatggtt
     2821 ggtgaaggcg acggcgttgc cggtgatggg cgaggcgacg gcgagcgcta cctcgcgcgg
     2881 cacctccggc cggtcgtcat cggcgagata ggcggcgagc gcgtcctcga tcgtcggata
     2941 gtcatcgcag gcgagaacgc ggatgcggtc ggtccggccg tcggcgtcgg cccaggcgaa
     3001 gcgcgcgttg gtcgcgccga tgtcgccgat gaggcggcgc atgatcgtct cgctcgacta
     3061 atgtacggct tcggtccggg cgagcgcctc gacgaaattc tcccgccagg ccgtgatgtc
     3121 gttgcgcttc agtgttccca tcatcgattg ccagcgttcg attcgctcgc cgagcggcat
     3181 ggccagggcc tcatgcagcg cttccgccat cccgtcaatg tcgaaggggt tgacgaggag
     3241 cgcatcgcgc agctcgcgcg cggcgccggc gaagcaggac agcaccagca cgcctggatc
     3301 gccgccatcc tgcgccgcca cgtattcctt ggcaacgagg ttcatgccgt cgcggatcgg
     3361 cgtcacgaga ccgatgcgcg ccgcgcgcag gaagccgcac agcgcgcgct ggttgaagct
     3421 cttgttgaga tagcgcagcg gcatccagtc gaactcggcg aagcggccat tgatgtgacc
     3481 ggcggcggtt tcgaggttgc ggcggatctc gagatactcg ggcacttcgc tgcgcgtcgg
     3541 cggcgcgatc tgcatcaagg tgacgcggcc gcgcgtttcc gggaacatct cgaggagacg
     3601 ctggaatgcc ttgaagcgcg cttcgagccc tttcgaataa tcgaggcggt cgacgccgat
     3661 catgaggctg cgatcgcgca ggctgtcatg aaggcgccgc atgtgacgcg cacccacgga
     3721 ctgcgccgct tgcgtcgcga tcgcctgaac atcgataccg atggggaagg ccgccgcgcg
     3781 caaccggcgt ccgaaggcgc tgatctcgcc ctggcggccg agctcggcgc cgctccagcg
     3841 caggagatag tcgcgaaagg cgctgaggtc gtcctcggtc tggaagccga cgagatcgta
     3901 agcacagagc gcgcggatga tcttggcgtg ggtcgggagg atccggaaca cctcggcggc
     3961 cgggaacggc gtgtgcagga agaagccgat cggctgcttc acacccaggc ggcgcagctc
     4021 gtcgccgagc gggatcaggt gatagtcatg cacccagatg aagtcgtcgt cgcgcagcaa
     4081 aggcgcgagc tgcgcggcga actggcggtt gacgcgcatg tagcccgcga aatcggcgcg
     4141 ctggtagtcg acgaaaccgg gacgataatg aaagagcggc cagagaacgc gattggcgaa
     4201 gccgttgtag tattgctcgt agtcctggcg cgcgagatcg agcgtggcgt agacgaagcc
     4261 gtcgccttcc accagcacgg gcgccgtcgc gggcgtcgcc gtcacctcgc cgctccagcc
     4321 gaaccagatg ccgccgctgc tgcgcaaggc
//
LOCUS       DASYNS010000394         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3688303, whole genome shotgun sequence.
ACCESSION   DASYNS010000394 DASYNS010000000
VERSION     DASYNS010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3688303"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..838
                     /locus_tag="VGP50_12280"
     CDS             68..838
                     /locus_tag="VGP50_12280"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEV8028201.1"
                     /translation="MAEMTMAAGASRIGVYGARLGFALAVLSLILLALAPIGWRTGLW
                     HFRTSFWYLMAPAAWVGIAAGVVSLVSLFWWGAIETGGRIMALVGLVIGGVMFYVPWS
                     FYHTLNTVPRIHDITTDTENAPAFSAAILTARAAEKGNSTDYDTKVGAQQKQGYPDLA
                     PVTTTLAPAQAYERALAAAQGMSGWRIVTNDPADGRIEASQSTLFMGFTDDIVIRVTP
                     DGSGSRIDMRSESRQGMSDFGVNAGRVRRYMAALKAQL"
     gene            985..1734
                     /locus_tag="VGP50_12285"
     CDS             985..1734
                     /locus_tag="VGP50_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8028202.1"
                     /translation="MTGRLAGKKALVTAAAQGIGRATALAFAAEGASVVATDINLAKL
                     EELTPAGAGKIEIRRLDVTDAAAIGALAQTLGAIDVLFNCAGFVHHGTILDCGETEWD
                     ATLDVNLRSMYRMIRAFLPAMLQRRQGASIVNMASAVSSVKGAPNRFVYGTSKAAVIG
                     LTKSVAIDFIKDGIRCNAICPGTVETPSLGERIAAQGGGPEVRQAFIARQPVGRLGTA
                     QEVANLALFLASDEASYITGQAHLVDGGMAL"
BASE COUNT          274 a          582 c          625 g          285 t
ORIGIN      
        1 tcgcctctcg ggggagggca tgctaggctt tgctcaggcc tgcgcgacga tgggccggga
       61 gggaagcatg gcggaaatga cgatggccgc cggtgcgagc cgcatcgggg tctatggggc
      121 gcggctgggc tttgcgctcg cggtgctgtc gctgatcctg ctggcgcttg cgccgatcgg
      181 ctggcgcacc gggctctggc attttcgcac gtccttctgg tacctgatgg cgccggccgc
      241 ctgggtcggc atcgccgccg gggtggtctc gctggtgtcg ctcttctggt ggggagcgat
      301 cgagacgggc gggcgcatca tggcgctggt cggtctcgtc atcggcgggg tcatgttcta
      361 cgtgccctgg tccttctacc acacgctcaa caccgtgccg cgcatccacg acatcaccac
      421 cgacaccgag aacgcgccgg ccttctccgc cgcgatcctg acggcgcgcg ccgccgagaa
      481 gggcaacagc accgattacg acaccaaggt cggcgcgcag cagaagcagg gctatcccga
      541 tctcgcgccg gtgacgacga cgctggcgcc ggcgcaggcc tatgagcgcg cgctggccgc
      601 ggcgcaaggc atgtcgggct ggcgcatcgt caccaatgat cccgcggacg ggcgcatcga
      661 ggcgagccag tcgacgctgt tcatgggctt caccgacgac atcgtcatcc gcgtcacgcc
      721 ggacggttcg ggaagccgca tcgacatgcg ctccgagagc cgccagggca tgagcgattt
      781 cggcgtcaac gccgggcgcg tccgcagata catggctgcg ctgaaggcgc agttgtagac
      841 gggtctcggc actttccaag atcgtcatcc ccgcgaaagc ggggacccag gggccaccgg
      901 cccgtcgctc gccctgggtc cccgcttcgc ggggatgacg gaggtagttg aacgagagcc
      961 atactcagga cggagaggcg ggagatgacg gggagactcg ccggcaagaa ggcgctggtg
     1021 acggcggcgg cgcaaggcat cggacgcgcc acggcgctgg cgtttgcggc cgaaggggcg
     1081 agcgtggtcg cgaccgatat caacctcgcg aagctcgagg agctgacgcc ggcgggtgcg
     1141 ggaaagatcg agatccggcg tctcgacgtc accgatgcgg cggcgatcgg cgcgctggcg
     1201 caaacgctgg gcgcgatcga cgtgctgttc aactgcgccg gctttgtcca tcacggcacc
     1261 atcctcgatt gcggcgaaac ggagtgggac gcgacgctcg acgtcaatct ccgctcgatg
     1321 taccgcatga tccgcgcttt tctcccggcg atgctgcagc gccgccaggg cgcttcgatc
     1381 gtcaacatgg catcggcggt ctcgtcggtg aaaggcgcgc ccaaccgctt cgtctacggc
     1441 accagcaagg cggcggtcat cggcctcacc aagtcggtcg ccatcgattt catcaaggac
     1501 ggcatccgct gcaacgccat ctgccccggc accgtcgaga cgccgtcgct tggcgagcgc
     1561 atcgccgcgc agggcggcgg tcccgaagtg cgccaggcct tcatcgcgcg ccagccggtg
     1621 ggccgcctcg gcaccgcgca ggaggtcgcg aacctcgcgc tctttctcgc ctcggacgag
     1681 gcgagctaca tcacgggcca ggcgcatctg gtcgatggcg gcatggcgct gtgaggcgac
     1741 ggcgagccgc ctctttgtga ttttct
//
LOCUS       DASYNS010000395         7425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1346059, whole genome shotgun sequence.
ACCESSION   DASYNS010000395 DASYNS010000000
VERSION     DASYNS010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346059"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VGP50_12290"
     CDS             <1..393
                     /locus_tag="VGP50_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8028203.1"
                     /translation="MLKPGKGFLGETSVPEQMPAWLTEADIAYGVETYKRTGFRGGLN
                     WYRNIDRNWELSASWDGLKIQQPALFIGGEDDGVIKAATSAKALEELSANVPGLKRKY
                     LIPATGHWVQQQRPTEVSAAVLEFLRGI"
     gene            complement(455..1744)
                     /locus_tag="VGP50_12295"
     CDS             complement(455..1744)
                     /locus_tag="VGP50_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007396495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0569 family flavin-dependent
                     oxidoreductase"
                     /protein_id="HEV8028204.1"
                     /translation="MADSIFRAAPLSVPVIIVGGGQAGLSMSWHLRKKGIEHLILERH
                     VACHAWRAERWDTFCLVTPNWQCLLPGFHYSGDDPHGFMLRGEIVDYLDAWIASFDPP
                     LRTGVEVRKLRRGQHGDFLLSTSAGDFVAQQVVVASGGYDEPIVPRYAERLDPDVLQL
                     HSSDYRNPRALPPGEVLVVGSGQSGCQIAEDLHLEGRRVHLCVGNAPRVARRYRGKDV
                     VAWLDLMGYYEMPVEQHPLKEGVRDNTNHYVTGRDGGRDIDLRLRALEGMQLYGRIAG
                     IGEGKLLFETDLRENLDAADTVSENIKRSIDGFIAKQGIAAPAETPYQPVWQPEQETP
                     ELDYRRANITSVVWCIGFRANFRWVEVPVFDGRGAPAHRRGVTREDGLYFLGLPWLHT
                     WGSGRLSGVGRDAAYLADHIAARIDAPAALNVEAMGA"
     gene            complement(1744..2046)
                     /locus_tag="VGP50_12300"
     CDS             complement(1744..2046)
                     /locus_tag="VGP50_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0570 family nitrogen starvation response
                     protein"
                     /protein_id="HEV8028205.1"
                     /translation="MPEMRFHLRWPDGSSTSCYSPSLVVGDYLRPGAAYPLADFVDRS
                     RQALRSASARVAAKYGFACSRALRQLEEIERRAQTFAGTAGAEVVVLGFDSSGEPW"
     gene            complement(2039..3226)
                     /locus_tag="VGP50_12305"
     CDS             complement(2039..3226)
                     /locus_tag="VGP50_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0565 family glycosyltransferase"
                     /protein_id="HEV8028206.1"
                     /translation="MTAERPLRIAMLTHSTNPRGGVVHALEVADALTDLGHEVSVHAP
                     DPLGMGFFRATRCRTVPIPARLVSGDLARLVETHVADYVSYFAAAPRERFDIYHAQDG
                     ISANALAALRDRGMVPGFLRTVHHIDDFADPRLAAQQKRGIDAADRVLCVSRLWQNII
                     ARDFGIAASRVGNGVDTARFSPASGRADAALRARLGWGQGPVYLAVGGIEKRKNTSRI
                     LRAFLRVRQHQPAAQLVVAGGASLLDHSAARASFDAILRESGLAAAGEGAPVVLTGPL
                     PDCDMPALFRLADALVFPSLAEGFGLVVLEAMASGTPAIVPRQAPFTEYLGEDDCLWV
                     DPMSVDAIAAAMVGAADPSTRRQLTAAGLDVARRHGWSAVATAHLQIYAECLRWCREA
                     ADA"
     gene            complement(3223..4233)
                     /locus_tag="VGP50_12310"
     CDS             complement(3223..4233)
                     /locus_tag="VGP50_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007398735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sll0787 family AIR synthase-like protein"
                     /protein_id="HEV8028207.1"
                     /translation="MRAARPRRALDLAALATELRAGRGIAHKRDIAAIARPLSLPAAP
                     VRVGDDCAAIPDGDGYLLFAIEGFINEFVEAEPWFAGWCGVMVNVSDIYAMGGRPVGV
                     VDALWSRNSIRAQPLLEGLAAAAKAYGVPVVGGHTNCQSPRGEQLSVAIIGRARQLLT
                     SFDAQPGDRLVAAIDLRGRYREPHPYWDASTGAAPDGERLRGDLEILPQLAEAGLCRA
                     AKDISMGGLAGTALMLLECSNLGAVIDVAAVPRPPGAPLARWLSSFPSYGFLLAVAHA
                     DVGAVCDRFHQRGIAAADIGLCDASGVVQLAEGSSDNPAMFWDLRQEPLLGISSAARV
                     FA"
     gene            complement(4230..4775)
                     /locus_tag="VGP50_12315"
     CDS             complement(4230..4775)
                     /locus_tag="VGP50_12315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0567/Sll0786 family nitrogen starvation
                     N-acetyltransferase"
                     /protein_id="HEV8028208.1"
                     /translation="MFLDLQDRPRPFLASEFRIHLAAEPWEHAGHARLRREVFCDEQG
                     IFRGDDRDALDGVALPLVAVAMVMNMPDQVVGTVRIHEAEAGLWYGSRLAVHRDFRRL
                     AFLGSGLISLAVGTARARGCIRFLAHVQAANVALFERLHWRSLESLTLHDRPHHVMEA
                     DLSQYPPCWTGELAPLRRSAA"
     gene            complement(4786..5898)
                     /locus_tag="VGP50_12320"
     CDS             complement(4786..5898)
                     /locus_tag="VGP50_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0568 family radical SAM protein"
                     /protein_id="HEV8028209.1"
                     /translation="MPAQPRPAEPQFLITELQSLGLRLAEPSAGAPGRRGGAGPSDHK
                     AVTLGSVTAMVPVHNDPAQASPYVAAAPDARGVSTLLRDGVAVGELRFPRQPRFYRLQ
                     TFDGVPYWKIAVLHGADVLATTVQQTCIRYAARETSCQFCAIGQSLAEDRTIARKTPE
                     QLAEVARAAVLLDGIRHMVLTTGTPNNSDRGAALLCDSAFAIKAAVPDLPIQGQCEPP
                     ADPVWFRRLRASGIDALGMHLEAVTPEIRARIMPGKAKVAISRYLAAFMAAVPVFGRG
                     QVSTYILAGLGDPPEAILAMCERLIAIGVYPFVVPFVPIAGTPLAAHPAPPPAFMRAL
                     LAPLGAMLAAAGLRSADIKAGCGRCGACSSLAGYER"
     gene            complement(5822..6922)
                     /locus_tag="VGP50_12325"
     CDS             complement(5822..6922)
                     /locus_tag="VGP50_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000257 - nitrilase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nit6803 family nitrilase"
                     /protein_id="HEV8028210.1"
                     /translation="MTSSSSAKSIRVAAVQLAPVFDRDGSGTLDKVCRAIRDVASKGA
                     RLVVFPETCVPYYPYFSFVRPPAQMGADHMRLYEGAVSVPGPVTEAVSAASRAQGIVV
                     VLGVNERDHGTLYNAQLIFDADGRLLLKRRKITPTFHERMIWGQGDGSGLRVVETAVG
                     RVGALACWEHYNPLARYALMAMHEEIHCSQFPGSLVGQIFADQMEVTIRHHALESGCF
                     VVNATGWLTEEQIAAVTPDPALQRTLRGGCCTAVVSPEGVHLCPPLREGEGAAIADLD
                     LSLIVKRKRMMDSVGHYARPELLHLTLDARATAPLVASAAATPSFLSEPISENRHGEP
                     FAHTAPADAGATPASGAAIPDHRTAIARVASG"
     gene            complement(6987..>7425)
                     /locus_tag="VGP50_12330"
     CDS             complement(6987..>7425)
                     /locus_tag="VGP50_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003630856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MSMEG_0572/Sll0783 family nitrogen starvation
                     response protein"
                     /protein_id="HEV8028211.1"
                     /translation="VDYEEKVFEDVKAKPGEKALVTFHTVAFEGSIGLVNLLQATRLL
                     RKGFETSVLLYGPGVTLGLQRGFPTLGDEAFPGHQAMNKQVGKFLEEGGKVYACRFAL
                     QALYGHGEPSLIPGIRPISPLDVLDLILIHKRDNAVIINTWTV"
BASE COUNT         1284 a         2476 c         2637 g         1028 t
ORIGIN      
        1 atgctgaagc cgggcaaggg cttccttggc gagaccagcg ttccggagca aatgccggcc
       61 tggttgaccg aggcggatat cgcctatggc gtcgaaacgt acaagcgcac cggcttccgc
      121 ggcgggctca attggtatcg caatatcgat cgcaattggg agctgtccgc gtcctgggac
      181 ggactgaaaa ttcagcagcc cgcgctcttc atcggcggag aagacgatgg cgtgataaag
      241 gccgccacga gcgccaaggc tctcgaggag ctgtcggcca atgtgcccgg gctgaagcgg
      301 aaatacctca tcccggcgac cgggcactgg gtccaacaac agcgcccgac cgaagtaagc
      361 gcggcggtgc tggaattcct gcgagggatt tgaccggtct tttttgcgcc gcggccgcga
      421 cgcaggcgcc cgcgacgcag gcgcttgatc agaactaagc gcccatcgct tcgacgttga
      481 gcgcggcggg ggcgtcgatg cgggcggcga tgtggtcggc gagataggcg gcgtcgcggc
      541 cgacgcccga caatcggccc gacccccagg tatggagcca gggcaggccg aggaagtaga
      601 ggccgtcctc gcgcgtcacg ccgcgcctat gcgcaggcgc gccgcggcca tcgaacactg
      661 gcacttcgac ccagcggaaa ttcgcgcgaa agccgatgca ccagaccacg ctggtgatgt
      721 tcgcgcggcg atagtcaagc tcgggcgttt cctgttccgg ctgccacacc ggctggtaag
      781 gcgtttccgc cggcgccgcg atgccctgct tggcgatgaa gccgtcgatg ctgcgcttga
      841 tgttctcgga gaccgtgtcg gccgcgtcga gattctcgcg cagatcggtc tcgaagagca
      901 gcttcccctc gccgatcccg gcgatacggc cataaagctg catgccttca agcgcgcgca
      961 ggcgcagatc gatgtcccgc ccgccatcgc gcccggtgac gtaatggttg gtgttgtcgc
     1021 gcacgccttc cttgagcggg tgctgctcca ccggcatctc gtaatagccc atgaggtcga
     1081 gccaggcgac gacgtccttg ccgcggtagc gccgcgccac gcgcggtgcg ttgccgacgc
     1141 agagatggac ccgcctgccc tcgagatgca aatcctcggc gatctggcag ccggattgac
     1201 cgctgccgac gaccagcacc tcgccgggcg gcagcgcgcg cggattgcga tagtcggaag
     1261 aatgcagctg cagcacatcc ggatcgagcc gctcggcata gcgggggacg atcggctcgt
     1321 cgtagccgcc cgaggccacg accacctgct gcgccacgaa gtcgccagcg ctggtcgaga
     1381 gcaggaaatc cccatgctgg ccgcggcgca gcttgcgcac ctcgacgccg gtgcggagcg
     1441 gcggatcgaa agaggcgatc caggcatcga gatagtcgac gatctcgccg cgcagcatga
     1501 agccatgggg atcgtcgccg gaataatgga agcccggcaa caggcactgc cagttgggcg
     1561 tcacgaggca gaacgtgtcc cagcgctccg cgcgccaggc gtggcaggcg acatggcgct
     1621 cgaggatcag atgctcgatg cccttcttgc gcagatgcca gctcatggac aggccggcct
     1681 gcccgccgcc gacaatgatc accgggacgg agagcggcgc agcgcggaag atcgaatcgg
     1741 ccatcaccag ggctcccctg aggagtcgaa gccaagcacg acgacttctg ctcccgccgt
     1801 gccggcgaag gtctgggcgc ggcgctcgat ctcttcgagc tggcgcagcg cgcgtgagca
     1861 ggcgaagccg tatttcgccg cgacgcgagc gctggcgctc cgcagcgcct ggcggctccg
     1921 gtcgacgaaa tcggcgagcg gataggcagc gccgggccgc agatagtcgc cgacgacgag
     1981 cgacggggaa tagcagctcg tgctgctgcc atccggccag cgcaggtgaa agcgcatttc
     2041 aggcatcggc cgcctcccgg caccatcgca gacactccgc atagatctgg agatgcgccg
     2101 tcgcgacggc ggaccagccg tgacgtcgcg cgacatcgag ccccgccgcc gtgagctgcc
     2161 ggcgcgtcga cggatccgcg gcgccgacca tcgctgccgc gatcgcgtcg accgacatcg
     2221 ggtcgaccca gaggcaatcg tcctcgccga gatattcggt gaaaggcgcc tggcgcggga
     2281 cgatcgccgg cgtgccgctg gccatggcct cgagaaccac gagaccgaag ccctcggcga
     2341 gcgacggaaa gaccagcgca tcggcgagcc gaaagagcgc cggcatgtcg caatccggca
     2401 gcggaccggt gaggacgacc ggcgccccct caccggccgc cgcgagaccg ctctcgcgca
     2461 ggattgcgtc gaagctggcg cgcgccgcgc tgtggtcgag aagactggca ccgccggcga
     2521 cgacgagctg cgccgccggc tggtgctgcc gcacgcgaag aaacgctcgg agaatgcgcg
     2581 acgtgttctt gcgcttctcg ataccgccca cggcgaggta gaccggcccc tgaccccagc
     2641 cgagacgcgc ccggagggcc gcgtccgcgc gaccggaagc cggcgagaag cgcgcggtgt
     2701 cgacgccatt gccgacgcgc gaagcggcga tgccgaagtc gcgcgcgatg atattctgcc
     2761 agaggcggga gacgcagagc acgcggtccg ccgcgtcgat cccccgcttc tgctgcgccg
     2821 cgaggcgcgg gtcggcgaag tcgtcgatgt gatggaccgt gcgcaggaag cccggaacca
     2881 tgccgcggtc gcgcaaagct gcgagcgcat tggcgctgat gccgtcctgg gcatgataga
     2941 tgtcgaagcg ctcgcgcggc gcggcggcga aatagctgac atagtccgcg acatgcgtct
     3001 ccacgagcct ggcgaggtcg cccgacacca gccgggccgg gatcggcacg gtgcggcacc
     3061 gcgtcgcgcg aaagaaaccc atgccgagcg gatcgggcgc gtgcacggag acctcgtggc
     3121 caagatccgt caaggcgtcg gcgacctcca gcgcatgcac gacgccgccc cgcggattgg
     3181 tggagtgggt cagcatggcg atgcgcagcg gtctttcagc ggtcatgcga aaacccgagc
     3241 ggcgctggag ataccgagca acggctcctg ccgcagatcc cagaacatcg ccgggttgtc
     3301 gctgctgcct tcggcaagct gcaccacgcc cgacgcgtcg cagagaccga tatcggcggc
     3361 ggcgatgccg cgctggtgaa agcggtcgca gacggcgccg acatccgcgt gggccacggc
     3421 gagaaggaag ccatagctcg gaaacgagga aagccagcgc gcaagcggcg cgcccggcgg
     3481 ccgcggcacg gccgccacat cgatgacggc gccgagattc gagcattcga ggagcatcag
     3541 cgccgtgccc gcgagaccgc ccatgctgat gtccttggcg gcacgacaga gcccggcctc
     3601 ggcgagttgc ggcaagatct cgagatcgcc gcgcaagcgc tcgccatcgg gcgccgcgcc
     3661 ggtggacgcg tcccaatagg gatgcggctc gcggtagcgg ccgcgcagat cgatcgccgc
     3721 caccagacgg tcgcccggct gcgcgtcgaa actggtgagg agctgcctgg cacggccgat
     3781 gatggccacc gagagctgct cgccgcgcgg gctctgacaa ttcgtatggc cgccgacgac
     3841 agggacgcca taggcctttg cggctgccgc cagcccctcg agcagcggct gcgcgcggat
     3901 gctgttgcgg ctccaaagcg catcgacgac gccgacggga cggccgccca tggcgtagat
     3961 gtcgctgaca ttcaccatga caccgcacca gccggcgaac cagggctcgg cctcgacgaa
     4021 ttcgttgatg aagccctcga tggcgaagag caggtagccg tcgccatcgg gaattgctgc
     4081 gcagtcatcg ccgacgcgga ccggcgccgc cggcagcgac aggggcctgg cgatcgcagc
     4141 gatatcccgc ttgtgcgcga tgccgcggcc ggcgcgaagc tccgtggcga gcgccgccag
     4201 gtcgagcgcg cgccgcggcc tggcggccct catgcggcag agcgccgcag cggcgcgagc
     4261 tcgcccgtcc agcagggcgg atactgcgaa agatcggcct ccatcacgtg atggggacgg
     4321 tcatgcagcg tcaaggactc gagcgagcgc caatgcagcc gctcgaacag cgccacattg
     4381 gcggcctgca catgggcgag gaagcggatg cagccgcgcg cccgcgccgt gccgacggcg
     4441 aggctgatca agccggagcc gagaaacgcc aggcgccgga agtcgcgatg gacggcgagg
     4501 cgcgacccgt accagagccc ggcttccgct tcgtgaatac gcacggtgcc gacgacctgg
     4561 tccggcatgt tcatcaccat cgccaccgcg accagcggca gggcgacgcc atcgagcgcg
     4621 tcccgatcat cgccgcggaa gatgccctgc tcgtcgcaga agacctcgcg ccgcaatcgc
     4681 gcatggcccg catgttccca gggctcggcg gcgaggtgga tgcggaactc gctcgccagg
     4741 aagggacgcg gccggtcttg cagatcgaga aacatcgcgg cgccctcagc gctcgtagcc
     4801 cgcgagcgac gagcaggcgc cgcaccggcc gcatccggcc ttgatgtccg ccgagcgcag
     4861 acccgcggcc gccagcatgg cgccgagcgg cgctagcagc gcgcgcatga aagcgggcgg
     4921 aggcgccgga tgcgccgcga gcggcgtgcc ggcgatcggc acgaagggca cgacgaaggg
     4981 atagacaccg atggcgatga ggcgctcgca catcgcgagg atcgcttcgg gcggatcgcc
     5041 cagtcccgcg aggatatagg tgctgacctg gccgcggccg aacaccggca cggcggccat
     5101 gaaggcggcg agatagcgcg agatcgcgac cttggccttg cccggcatga tgcgggcgcg
     5161 gatttcgggc gtgaccgctt cgagatgcat gccgagcgcg tcgatgccgg acgcgcgcag
     5221 gcggcggaac cagacgggat cggcgggcgg ctcgcattgt ccctggatcg gcaaatcggg
     5281 aacggcggcc ttgatggcga aggcgctgtc gcacagcagc gccgcgccgc ggtcgctgtt
     5341 gttgggcgtg cccgtggtga gcaccatgtg gcggatgccg tcgagcagca ccgccgcccg
     5401 cgccacctcg gcgagctgct ccggcgtctt gcgcgcgatc gtgcgatcct cggcgagcga
     5461 ctggccgatg gcgcagaact ggcaggaggt ctcgcgggcg gcatagcgga tgcaggtctg
     5521 ctggaccgtc gtcgcgagca cgtcggcgcc gtgaagcacc gcgatcttcc agtagggcac
     5581 gccgtcgaag gtttggaggc gatagaagcg cggctggcgc ggaaagcgga gctcgcccac
     5641 tgcgacgccg tcgcgcagca gcgtgctgac gccgcgcgcg tcaggcgccg ccgcgacata
     5701 aggcgaggcc tgcgccgggt cgttgtgcac cggcaccatc gcggtgacac tgcccagcgt
     5761 caccgccttg tggtcggaag gaccggcgcc gccgcgccgc cccggcgcgc cggccgaggg
     5821 ctcagccaga cgcaacccga gcgattgcag ttcggtgatc aggaattgcg gctccgctgg
     5881 ccggggttgc gccggcatct gccggagcgg tgtgagcaaa gggctctcca tggcggttct
     5941 cgctgatggg ctctgagagg aaggaagggg tggccgccgc gctggcgacg agcggcgcgg
     6001 tagcgcgggc gtcgagcgtg agatgcagca gctcgggccg cgcataatgg ccgacggaat
     6061 ccatcatgcg cttgcgcttg acgataagcg acagatcgag atcggcgatg gccgcgcctt
     6121 cgccttcgcg gagcggcgga cagagatgca cgccttcggg cgagacgacg gcggtgcagc
     6181 agccgccgcg cagcgtgcgc tgcagcgccg ggtcgggcgt cacggcggcg atctgctcct
     6241 ccgtcagcca gccggtggcg ttgacgacga agcagccgga ttcgagcgca tgatggcgga
     6301 tcgtcacttc catctggtcg gcgaagatct gtcccaccag cgagccgggg aactggctgc
     6361 aatggatctc ctcgtgcatt gccatgagcg cgtagcgcgc gagcgggttg taatgctccc
     6421 agcaggcaag cgcgccgacg cggccgaccg cggtctcgac cacgcgcaag ccgctgccgt
     6481 cgccctgccc ccagatcatg cgctcatgga aggtcggcgt gatcttgcgc cgcttgagga
     6541 gcagccttcc gtcggcatcg aagatgagct gcgcgttgta gagcgtgcca tgatcgcgct
     6601 cgttcacgcc gagcacgacg acaatgccct gcgcgcgcga agccgcgctc accgcctcgg
     6661 tgaccggccc cggcacgctg accgcgccct cataaaggcg catatgatcg gcgcccattt
     6721 gcgcgggcgg gcgaacgaag gagaaatagg gatagtaagg gacgcaggtc tccggaaaca
     6781 cgacgaggcg cgcgcccttc gacgcgacgt cgcggatggc gcggcacacc ttgtcgagcg
     6841 tgccgctgcc gtcgcgatcg aagaccggag cgagctgcac cgcagcgacc ctgatggatt
     6901 tcgcgctgct gctggaggtc atggcacttc tcccgcggcg tcagcgccgc ggcagcaagg
     6961 gggttccctt gctgccgcgc gcgcggtcag acggtccagg tgttgatgat cacggcgttg
     7021 tcgcgcttgt ggatcaggat gaggtcgagc acgtcgagcg ggctgatcgg ccgaattccc
     7081 gggatcagcg acggctcgcc atggccgtac agcgcctgaa gcgcgaaacg gcaggcatag
     7141 accttgccgc cttcctcgag gaacttgccg acctgcttgt tcatggcctg atgcccgggg
     7201 aacgcttcgt cgccgagcgt cgggaagccg cgctgcaggc cgagcgtcac gccggggcca
     7261 tagagcaaca ccgaggtctc gaatcccttg cgcaacagcc gcgtcgcctg caacaggttc
     7321 accaatccga tcgatccttc gaaagcgacg gtgtgaaagg tcacgagcgc tttctcgccc
     7381 ggcttggcct tgacgtcctc gaagaccttc tcctcatagt cgacg
//
LOCUS       DASYNS010000396         3838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1503776, whole genome shotgun sequence.
ACCESSION   DASYNS010000396 DASYNS010000000
VERSION     DASYNS010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503776"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VGP50_12335"
     CDS             complement(<1..1314)
                     /locus_tag="VGP50_12335"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HEV8028212.1"
                     /translation="MRNAFIDTLPQFETAYLNRREAIYQSVTTFGSLFNRDYIIRCFR
                     KMLALHTLMGGLPSMVDCAGSEPLKDLVTMGGQAARAGGAIGQPTASTVGHRIGRVRD
                     REGMMSFRAFDRVKDLSVGLRVLIVALLPMLGMLGLSGQILLTKRHEVVEMGKLAQLA
                     ALVNKISAFTHELQKERGMSSVLISSQGAQFRGEVDAQRRTTDAQKAVFEQATTALDA
                     AAFGADFARKTEIATRDVARLAAIRQQISALQLSGPDSFAYYTSTIAELFDIVAEVPV
                     VIKNTEVPRAVMADFLLMEAKESAGQERATGAQGFAAGKFNEALLRRFIFLGSLQDTF
                     FRLFDSYASAEERALLEQTLTPATLAELQRLRKIAAEGGLAGELQGVTGQSWWQAATD
                     RIDLMKKVEDRLAANLATTAQRVSGAAVTEFNVVLAIVLGLLIVSG"
     gene            1298..2506
                     /locus_tag="VGP50_12340"
     CDS             1298..2506
                     /locus_tag="VGP50_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HEV8028213.1"
                     /translation="MKAFLKSGHLPTLFAAFLYFDLSFMVWVMLGPLGVQIARDLGLS
                     AAQKGVMVATPVLAGALIRVVNGVLVDHLKAKLTGTISQMVVICGLLAAWWIGVHSFA
                     QTLLVGIVLGVAGSSFAVALPLASRWYPPEHQGTALGIAGAGNSGTVLAALFAPGLAL
                     AFGWNAVFGLAVLPLVATLAIYLWLAKDSPSCPPPKSLPEYMSVLKIGDAWSFMFFYG
                     VTFGGFVGLASSLTIYFNDQYGLSPVTAGYFTAACVFAGSMVRPVGGYVADRIGGVRS
                     LNIMYVVAAACLVVISFAPPQAWMALGVFIVAMLALGMGNGAVFQLVPQRFGREIGVM
                     TGLIGMTGGVGGFYLSASLGYSKQLTGDYQVGFLVFAILAALALLGLSRVKRRWRAAG
                     HAEVPVAARI"
     gene            2522..>3838
                     /locus_tag="VGP50_12345"
     CDS             2522..>3838
                     /locus_tag="VGP50_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV8028214.1"
                     /translation="MDRHAAARPRLVVVGNGMAGMRTVEELLKRRPDRYDITVFGAEK
                     QVNYDRILLSAVLAGEKELDEIVIHPRRWYDENGITLVADDRVVDIDRQSRTVTSASG
                     RVAGYDRLLLATGSQPLLPEIAGTGLAGVCTFRDVADVDTMIRAAREHRRAIVIGGGL
                     LGLEAANGLRKRGMAVTVVHLMDSLMERQLDQAAAALLRRELEQRGIDIRAGSEPAAI
                     LGSERALGVRLTDGCGLPGDLVVIAVGIRPNVDLARLAGLEIDRGIAVGDDLRSSDPA
                     IFAVGECVEHRGKTYGLVAPLWEMAKICADHLAAESGSPAAYAGSVPATRLKVTGIDV
                     FSAGDFRGDASTEDIVFRDASRRIYKRLVLRDDRLTGVVLYGDSRDAGWYVQLLRDGA
                     ELGELRDLMIFGQDYAAPAASIAVAAPIPLSPRTGEARGRRLAVARR"
BASE COUNT          566 a         1285 c         1284 g          703 t
ORIGIN      
        1 gccggaaacg atgagcaagc cgagaacgat ggcgagcacg acgttgaatt cggtcaccgc
       61 ggcgccgctg acgcgctgcg ccgtggtggc gagattggcg gccaggcgat cctcgacctt
      121 cttcatcagg tcgatccgat ctgtcgccgc ttgccaccag ctttgtccgg tgacgccctg
      181 caactcgccg gcgaggccgc cctccgccgc gatcttgcgc aggcgctgca gctcggccag
      241 ggtcgccggc gtcagcgtct gttcgaggag agcacgctcc tccgcgcttg catagctgtc
      301 gaacaggcgg aagaacgtgt cctgcagcga gccgaggaag atgaagcggc ggagcagcgc
      361 ctcattgaac ttgccggcgg cgaagccttg cgcgccggtg gcgcgctcct gaccggcgct
      421 ctccttcgcc tccatgagca ggaaatcggc catcaccgcc cgcggcacct cggtgttctt
      481 gatgacgacc gggacctcgg cgacgatgtc gaacagctcg gcgatcgtcg acgtatagta
      541 ggcgaacgag tcggggccgg acagctgcag cgcgctgatc tgctgtcgga tcgccgcgag
      601 ccgggcgacg tcgcgcgtgg cgatctcggt cttacgcgcg aagtcggcgc cgaaggccgc
      661 ggcgtcgaga gcggtcgtcg cctgttcgaa gaccgccttc tgcgcatcgg tcgtccggcg
      721 ctgggcgtcg acctcgccgc ggaactgcgc gccttggctg ctgatcagca cgctgctcat
      781 gccgcgctcc ttctgcagct catgcgtgaa agcgctgatc ttgttcacga gggcggccaa
      841 ctgcgcgagc ttgcccatct ccacgacctc atgacgcttg gtcaggagga tctggccgga
      901 gagtccgagc atgcccagca tgggcaacag cgccacgatc aagacgcgca ggccgacgct
      961 gagatccttc acccggtcga acgctctgaa gctcatcatc ccctctctat cccggacgcg
     1021 gccgatgcga tgcccaaccg ttgacgccgt cggctgacca atagcgccgc cggcgcgcgc
     1081 agcttgcccg cccatcgtta ccaaatcttt taggggttca ctgccggcgc aatcgaccat
     1141 tgatggcaga ccccccataa gggtgtgcag cgcaagcatt ttcctaaaac accgaatgat
     1201 ataatctcta ttgaataagc tcccgaacgt ggtgacactc tggtaaatag cctctctgcg
     1261 attgaggtac gcggtttcaa actgggggag cgtatcgatg aaagcgtttc tcaagtcagg
     1321 gcatctcccg acgctcttcg cggccttcct ctatttcgac ctcagcttca tggtgtgggt
     1381 gatgctggga ccgctcggtg tgcagatcgc cagggatctc gggctgagcg cagcgcagaa
     1441 gggcgtgatg gtggcgacgc cggtgctcgc cggcgcgctc atccgcgtcg tcaacggcgt
     1501 cctcgtcgat catctgaagg caaagctgac cggaaccatc agccagatgg tggtgatttg
     1561 cggcctgctc gcggcgtggt ggatcggcgt tcacagcttc gctcagacgc tcctggtcgg
     1621 gatcgtgctc ggcgttgccg gctcgtcctt tgccgtggcg ctgccgctgg cgtcgcgctg
     1681 gtatccgccg gagcatcagg gtacggcgct cggcatcgcc ggcgccggca attcgggcac
     1741 cgtattggcg gcgctcttcg cgcccggtct cgctctcgct ttcggctgga acgccgtctt
     1801 cggcctcgcg gtcctgccgc tcgtcgcgac gctcgcgatc tatctgtggc tggccaagga
     1861 cagcccgagc tgcccgccgc cgaaatcgct gcccgaatat atgagcgtct tgaagatcgg
     1921 cgatgcctgg tcgttcatgt tcttctacgg cgtgaccttc ggcggcttcg tcggcctggc
     1981 ctcgtcgctg accatctatt tcaacgatca atacggcttg agcccggtga cggcgggcta
     2041 tttcaccgcc gcctgcgtgt tcgccggctc catggtgcgg ccggtcgggg gctatgtcgc
     2101 cgaccgcatc ggcggcgtgc gctcgctcaa catcatgtac gtcgtggcgg ctgcgtgcct
     2161 ggtggtcatc agtttcgcgc cgccccaggc ctggatggcg ctcggcgtct tcatcgtcgc
     2221 catgctcgcg ctcggcatgg gcaacggcgc ggtgttccag ctcgtgccgc agcgcttcgg
     2281 ccgcgagatc ggcgtgatga ccggcctcat cggcatgacc ggcggggtcg gcgggttcta
     2341 cctctccgcc agcctcggct attcaaagca gctcaccggc gactaccagg tcggcttcct
     2401 cgtcttcgcg atcctggcgg cgctggcgct gctcgggctg tcccgggtca agcggcgctg
     2461 gcgggcagcc gggcatgccg aggtgcctgt cgcggcgcgg atctgaggcc ggggtagcgt
     2521 cgtggaccgg cacgccgccg cgcgcccgcg cctggtcgtc gtgggcaacg gcatggccgg
     2581 gatgcgcacg gtcgaggagc tgctgaagcg ccggccggat cgctacgaca tcacggtctt
     2641 cggggcagag aagcaggtca actacgatcg gatcctgctg tccgccgtgc tcgccggcga
     2701 gaaggagctc gacgagatcg tcatccatcc gcggcgctgg tatgacgaga acggcatcac
     2761 gctggttgcg gacgaccggg tggtcgacat cgaccgacag tctcgcaccg tcacctccgc
     2821 cagcggaagg gtggcgggct atgaccgcct gcttctcgcc actggatcgc agcccctcct
     2881 gccggagatt gccggcaccg gcctcgccgg cgtctgcacc ttccgcgacg ttgccgatgt
     2941 cgacacgatg atcagggcgg cgcgcgagca tcgccgcgcc atcgtcatcg gcggcggcct
     3001 gctcggtctc gaagcagcca atgggttgcg caagcgcggc atggcggtaa ccgtcgtcca
     3061 tctcatggac agcctgatgg agcgccagct cgaccaggcg gcggcagccc tccttcggcg
     3121 cgagctggag cagcgcggga tcgacatccg cgccggcagc gagcccgccg ccatcctcgg
     3181 cagcgagcgg gcgctgggcg ttcgccttac cgatggttgc gggcttcccg gcgatctcgt
     3241 ggtgattgcc gtcggcatcc gacccaatgt cgatttggcg cgtctggctg gtctcgaaat
     3301 cgatcgcggc atcgccgtgg gcgacgacct gcgcagctcc gaccccgcca tcttcgccgt
     3361 cggcgagtgc gtcgagcacc gcggcaagac ctatggcctg gtcgcgccgc tgtgggagat
     3421 ggccaaaata tgcgccgatc atctcgcggc ggagtcgggc agccccgcgg cctatgccgg
     3481 atcggtgccg gcaacccgat tgaaggtgac cggcatcgat gtgttctcgg ccggggattt
     3541 ccgcggcgac gcgagcaccg aggatatcgt ctttcgcgac gcgtcgcgcc gcatctacaa
     3601 gcgcctggtg ctgcgcgacg atcgacttac cggcgtcgtg ctctatggcg attcccgcga
     3661 cgccggctgg tacgtccaac tcctgcgcga cggcgcggaa ttgggagagc tgcgcgatct
     3721 catgatcttc ggccaggact acgccgcccc ggcggcgagc atcgccgtcg ccgcgccaat
     3781 cccgctctcg ccgcggacgg gcgaggccag aggccggcgg cttgcggtcg cgcgccgt
//
LOCUS       DASYNS010000397         5175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2595676, whole genome shotgun sequence.
ACCESSION   DASYNS010000397 DASYNS010000000
VERSION     DASYNS010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2595676"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..243)
                     /locus_tag="VGP50_12350"
     CDS             complement(28..243)
                     /locus_tag="VGP50_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028215.1"
                     /translation="MQIMTGWGIVPVTRVVSTQPVVILGTASGAPFEPGRRVTPARHG
                     SGSNLASDHGRFARPTYHRGRLLDLSV"
     gene            complement(358..978)
                     /locus_tag="VGP50_12355"
     CDS             complement(358..978)
                     /locus_tag="VGP50_12355"
                     /inference="COORDINATES: protein motif:HMM:NF017820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV8028216.1"
                     /translation="MRRRFGVSLTHRLAAPRERLDSEGDIAAFIARQPPMMRVLRAVA
                     AQRLPDGWVGAGFIRNAVWDALHGVSSPRDHGDVDVIFFDASDVRRERDAHIEGDLAA
                     AYPDVPWSVRNQARMHARNGDAPYADTADALRHWPERCTAIAARAAANGVELLAPFGV
                     GDLVALIVRPTPAFARKMNIYLERLGEKNWQARWPDLKVMEAKDVG"
     gene            complement(951..2147)
                     /locus_tag="VGP50_12360"
     CDS             complement(951..2147)
                     /locus_tag="VGP50_12360"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV8028217.1"
                     /translation="MPKYLTLDDIAPQGKRVLLRADLNVPMEHGQVSDGTRITRVAPT
                     IRALAERGAMVVVMSHFGRPKGKPDPAFSLRPLVAPLAQAIGRPVAFAEDCIGPAAER
                     IVASLRPGGVALLENLRFHPEEEANDRVFAEALARLGDIYVNDAFSAAHRAHASTEAI
                     AHLLPAVAGRLMQAELEALGAALETPQHPLAALVGGAKVSTKLDLLGFIIPKVDALVI
                     GGAMANTLLAAQGVSVGKSLCEHDMADAARRLVDQAKRAGCALLLPVDAVVAPALAAG
                     VASTTVPIDRVPADQMILDIGPRTVGAIADRLAACRTLVWNGPMGAFETPPFDAGTTA
                     VARRVAELTKAKKLLSVAGGGDTVAALAHAGVIDDLSYVSTAGGAFLEWLEGRTLPCV
                     AALACP"
     gene            2299..3339
                     /locus_tag="VGP50_12365"
     CDS             2299..3339
                     /locus_tag="VGP50_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV8028218.1"
                     /translation="MANTLEWLAYRASQGLRISWYFGQKWLSARLTSEMPAPPELKER
                     MPDRARILADLRALLEQDWRNIEEGHYRLPEDAVALRPLDALRKAGLFFSDLPRVDKR
                     RRSEAHQEVLQAAPPGRYPRYYLQNFHYQTDGYLSRHSAELYDHQVEVLFGGGADAMR
                     RQALVPLARILGERGVRGARLLDLACGTGRFLREVKSNYPKLAVTALDLSPYYLDLAR
                     AVLKPWSGTGFVEASAEAVPAADASFDVVTAIFLFHELPAQARQRVAAEAARLLRPGG
                     AVIFVDSIQLGDVPDYDPLVEYFPQAVHEPYYAGYAREDLAGLFAAAGLALEESRVAY
                     FSKIMTFRKAGS"
     gene            complement(3336..3752)
                     /locus_tag="VGP50_12370"
     CDS             complement(3336..3752)
                     /locus_tag="VGP50_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEV8028219.1"
                     /translation="MILFKLRCSAGHEFATWFRDGAAYERQSARSLISCPACGDTGID
                     KAPMAPRLGRRAAAPAETAPSADQLRQMLRALRQHVETHCDDVGDRFAEEARRIERGE
                     AERRGIYGEATEDEARALSDEGIEVGHIPWVPPNDA"
     gene            3818..4372
                     /locus_tag="VGP50_12375"
     CDS             3818..4372
                     /locus_tag="VGP50_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV8028220.1"
                     /translation="MPPVNASAAAAPPSTWVARFLPLIRAGGRVLYLAAGSGRHTQAL
                     RDKGYTVVAVDRDTAALRARFAADGGCAIVALDLEDGGPCRLGGGFDGIVVTNYLHRP
                     LLPALADALAQEGVLIYETFMAGNERLGKPSNPDFLLKPGELLAAFGGSLTVVAFEQG
                     IVLAPRPAAVQRLAAVKGALGQLP"
     gene            complement(4414..>5175)
                     /locus_tag="VGP50_12380"
     CDS             complement(4414..>5175)
                     /locus_tag="VGP50_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEV8028221.1"
                     /translation="AAGAELIMTPEVSDMMEPKRALRLAKAKDEATHPMLAACRALAR
                     ETGAWLLLGSAVVRRDDGSDDRLANRSFLIAPDGGIAARYDKIHMFDVELEGGESYRE
                     SNAFHPGEAAVLASLPWGVLGMTICYDLRFPHLYRALAQAGADFLSIPSAFTVPTGRA
                     HWHVLMRARAIETGCFVFSPAQWGEHAEGRKTYGHSLIVAPWGEILAEADDGTGFITA
                     DVDPVKITESRRKVPSLSHDRPFTAPKRARALAAE"
BASE COUNT          812 a         1781 c         1760 g          822 t
ORIGIN      
        1 aaccgacgcg gcgcggcagc cgccgcgcta gaccgacaga tcgaggagac gtccgcggtg
       61 gtaggtgggt cgggcgaagc ggccgtgatc cgacgcgaga ttggaccccg agccgtggcg
      121 ggcgggcgtg acacgccggc cgggctcgaa cggcgcaccc gatgcagtcc cgagaatgac
      181 gacgggttgc gtcgatacga cccgcgtcac tggaacgatc ccccatccgg tcatgatctg
      241 cacgctcaaa atccccccac ggaataggcc agactatacc acgtggacta agccaaaagc
      301 aatagtcaag tggataaatg gttaacctcc agtaactatg atgtaaaacg cggccggtca
      361 tccgacgtcc ttcgcttcca tgactttcag gtccggccat cgcgcctgcc aattcttttc
      421 accgaggcgc tccagataga tattcatctt gcgggcgaag gcgggggtcg ggcgcacgat
      481 cagcgccacg aggtcgccta ccccgaaggg tgcgagcagc tcgaccccgt ttgccgcggc
      541 gcgggcggcg atcgccgtgc aacgctccgg ccagtgacgc agcgcgtccg ccgtgtcggc
      601 atagggagca tcgccgttcc tggcatgcat gcgcgcttgg tttctcaccg accagggaac
      661 atccgggtat gccgccgcca aatccccctc gatgtgcgcg tcgcgctcgc ggcgcacatc
      721 gctcgcgtcg aagaatatca cgtcgacatc cccatggtcg cggggcgacg acacgccatg
      781 cagcgcatcc cagacggcgt tgcggataaa gcccgcgccg acccacccat cgggcaggcg
      841 ctgcgccgcg acggcgcgaa ggacccgcat catcgggggc tgccgcgcaa tgaaggcggc
      901 gatgtcgcct tcgctgtcga ggcgctcgcg cggcgctgcc aagcggtggg tcaaggacac
      961 gccaaagcgg cgacgcatgg cagggtccgg ccttccagcc attcgagaaa ggcgccgccc
     1021 gccgtcgaga catagctcag atcgtcgatg acccccgcat gggcgagcgc cgccaccgtg
     1081 tcgccgccgc ctgcgacact gagaagcttc ttcgccttgg tgagctcggc gacgcggcgc
     1141 gccaccgccg tggtgccggc atcgaaaggc ggcgtctcga acgcccccat cggcccgttc
     1201 cacaccagcg tccggcacgc ggcgaggcga tcggcgatcg ccccgaccgt gcgcggtccg
     1261 atatcgagga tcatctgatc ggccggcacg cggtcgatcg gcaccgtcgt gctcgcgacg
     1321 cccgccgcga gcgcgggcgc gacgacggcg tcgaccggca gcaggagcgc gcatcccgct
     1381 ctcttcgcct gatcgacgag gcggcgcgct gcgtcggcca tgtcgtgctc gcagagcgac
     1441 ttgccgacgg acaccccctg tgcggccagc aatgtgttgg ccatggcgcc accgatcacg
     1501 agcgcgtcga ccttggggat gatgaagccg agaagatcga gcttggtcga gactttggcg
     1561 ccgccgacga gggcggccag cggatgctgc ggcgtctcaa gcgcggcgcc gagggcctcg
     1621 agctccgcct gcatcagccg cccagccacg gcgggcagca gatgcgcgat cgcctcggtc
     1681 gaggcatggg cgcggtgggc ggcggaaaag gcgtcgttga cgtagatgtc gccgagccgc
     1741 gccagagcct cggcaaatac ccggtcattg gcttcttcct cggggtggaa gcgcaaattc
     1801 tcgaggagcg ccacgccgcc gggcctgagg gatgcgacga tgcgctccgc ggcagggccg
     1861 atgcaatcct cggcgaaggc gaccggccgc ccgatcgcct gcgccagcgg cgccaccagc
     1921 gggcgcagcg agaaggcggg atcgggcttg cccttgggcc ggccgaaatg ggacatgacc
     1981 acgaccatcg cgccgcgctc ggccagcgcg cgaatggtcg gggcaacgcg cgtgatccgg
     2041 gtcccgtcgc tcacctggcc gtgctccatg ggcacgttga gatcggcgcg cagcaacacc
     2101 cgcttcccct ggggcgcgat atcgtcgagg gtcagatact tcggcatggt ggctccgagg
     2161 ccggaaaaac ggggcggcac ctcctactaa cgccgtcgag cggcgtcaat tcgccttcag
     2221 atgtggctcc gcaatcccgg cgcgggctgc gacgtctcgc gtcttgccaa atccgccggc
     2281 cttcgccagc tataggccat ggccaacacg ctcgaatggc tcgcttatcg cgcttcgcag
     2341 gggctgcgca tatcctggta tttcgggcag aaatggttga gcgcgcgtct caccagcgag
     2401 atgccggcgc cccccgagct caaagagcgc atgcccgatc gcgcgcgcat cctcgccgat
     2461 ctgcgcgcgc tcctcgagca ggattggcgc aacatcgagg aaggacatta tcggctgccc
     2521 gaggacgcgg tggcgctgcg cccgctcgat gccttgcgca aggccggcct cttcttctcc
     2581 gatctgccgc gcgtcgacaa gcggcgccgc tcggaagcgc atcaggaggt gctgcaggcg
     2641 gcgccgcccg gtcgctaccc gcgctactac ctgcagaatt tccactatca gaccgacggc
     2701 tatttgagtc gccattcggc ggagctctac gaccatcagg tcgaagtgct gttcgggggc
     2761 ggcgccgacg ccatgcgccg gcaggcattg gtgccgctgg cgcggatcct tggagagcgc
     2821 ggcgtgcgcg gcgcccgtct tctcgatctc gcctgcggca ccggccgctt cctgcgcgag
     2881 gtcaagagca actatccgaa gcttgccgtc accgcgctcg acctcagccc ctattatctc
     2941 gacctggcgc gcgcggtgct caagccctgg tcgggcacgg gcttcgtcga agcctcggcg
     3001 gaagcggtgc ccgcggccga tgcgagcttc gacgtcgtga ccgccatctt cctctttcac
     3061 gagctcccgg cccaggcgcg gcagcgcgtc gccgcggaag cggcgcgact gctgcgcccg
     3121 ggcggcgcgg tcattttcgt cgattcgatc cagctcggcg acgtccccga ctatgatccg
     3181 ctggtcgaat attttccgca ggctgtgcac gagccatact acgcgggcta tgcccgcgag
     3241 gatctcgccg gcctctttgc cgctgccggt ctcgcgctcg aggaaagccg agtggcctat
     3301 ttctcgaaga tcatgacctt ccgcaaagcg ggctcctaag cgtcgttcgg cggaacccag
     3361 ggaatgtggc cgacctcgat cccctcgtcg ctgagcgcgc gcgcctcgtc ttccgtggcc
     3421 tccccgtaga tgccgcgccg ctcagcctcg ccgcgctcga tgcgccgcgc ctcttcggcg
     3481 aagcggtcgc cgacgtcgtc gcagtgagtc tcgacatgct ggcggagcgc gcgcagcatc
     3541 tggcggagct ggtccgccga gggtgcggtt tccgccggcg cagcggcacg tcggccgagc
     3601 cggggggcca tgggcgcctt gtcgatgccg gtgtcgccgc aagccgggca gctgatcagg
     3661 ctacgcgcgc tctggcgttc ataggcggcg ccgtcgcgga accacgtcgc gaactcgtga
     3721 ccggcactgc agcggagctt gaacagaatc atcgattgtc caaccctttc ctgacatctt
     3781 gctccggcga caacggctta gcaatcgccc ctgcgcaatg cccccggtta acgccagcgc
     3841 cgccgcggcg ccgccctcga cctgggtcgc ccgtttcctg ccgctgatcc gggcgggcgg
     3901 ccgcgtgctc tatcttgccg ccggcagcgg acgccatacg caagcgctac gcgacaaggg
     3961 ctacacggtg gtcgcggtcg accgcgatac ggcggcgctg cgcgcccgct tcgccgcgga
     4021 tggcggatgc gcgatcgtcg cgctcgacct cgaggatggc ggaccctgcc ggctcggcgg
     4081 cggcttcgac ggaatcgtcg tcaccaacta tcttcaccgg ccgctgctgc cggcgctggc
     4141 cgatgccctg gctcaggaag gtgtcctgat ctacgagacc ttcatggccg gcaatgagcg
     4201 gctcggcaag ccgagcaatc cggatttcct cctgaagccg ggcgagctgc tcgctgcctt
     4261 tggcgggtcc ttgacggttg ttgccttcga gcagggaatt gtcctggcgc cccgtccggc
     4321 ggcggtccag cgcctggcgg cggttaaggg cgcgctcggc caactgccgt agccgggaac
     4381 cagtctcgtt agcaggggcg acgcttgcgc gtcctattcg gccgccagtg cccgtgcgcg
     4441 cttcggcgcc gtgaagggcc ggtcgtggga gagcgagggt accttgcggc gggattcggt
     4501 gatcttcacc ggatcgacgt cggcggtgat aaagcccgtg ccgtcatccg cctcggcgag
     4561 aatctcgccc caaggggcga cgatcagaga gtggccgtag gtcttgcgtc cctcggcatg
     4621 ctcgccccat tgcgcgggag agaagacgaa gcagccggtc tcgatggcgc gcgcgcgcat
     4681 cagcacatgc caatgggcgc ggcccgtcgg cacggtgaag gccgagggga tcgacaggaa
     4741 gtccgcaccc gcttgcgcga gcgcccgata gagatgggga aagcgcaaat cgtagcagat
     4801 ggtcatgccg agcacgcccc agggcagcga tgccagcacg gccgcttcgc cgggatggaa
     4861 ggcattcgat tcgcgatagc tttcgccgcc ctcgagctcg acgtcgaaca tgtggatctt
     4921 gtcgtagcgc gcggcgatgc cgccatcggg cgcaataagg aaggagcgat tggcaagccg
     4981 gtcgtcgctg ccgtcatcgc gccgcaccac ggccgagccg aggagcagcc aggcgccggt
     5041 ctcacgcgcc agcgcgcggc aggcggcgag catgggatgc gtggcctcgt ccttcgcctt
     5101 ggcgaggcgc agcgcgcgct tcggctccat catgtcgctc acttcgggcg tcatgatgag
     5161 ctcggcgccg gccgc
//
LOCUS       DASYNS010000398         4103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4470344, whole genome shotgun sequence.
ACCESSION   DASYNS010000398 DASYNS010000000
VERSION     DASYNS010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4470344"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2094
                     /gene="parC"
                     /locus_tag="VGP50_12385"
     CDS             <1..2094
                     /gene="parC"
                     /locus_tag="VGP50_12385"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV8028222.1"
                     /translation="LKPVHRRVLYAMRELNLDPKSGYKKCSRVVGDVVGKFHPHGNEA
                     VYETLVRLAQDFAQRYPLVDGQGNFGNIDGDNAAAMRYTESRLTEVAEALLTGIGQDA
                     VDFRDTYDGENSEPTVLPARFPNLLANGASGIAVGMATSIPPHNAGELCDALAHLVKH
                     KRATIEELLALVPGPDFPTGGVLVENADVFRQAYETGRGSFRLRARWEVEKLAHGVYQ
                     IVVTEMPYQVPKARLIEKIAALLEERKLPLLADLRDESTDKVRLVLEPKTRNVDPGVL
                     MESLFRATELETRISLNMNVLDAAGVPRVMNLREVLQAFLDHRRTVLQRVTRFRLAEI
                     ARRSEILKGQMIVYLNLDEVIRIIREHDDAKERMMKRWRLSDVQAEAILNMRLRALRK
                     LEEIEIKKELKTLGEEQKDLSGLLEDDKRQWKAIAAELADIKKAFGGGALGKRRTEIA
                     AAPAPVQVPVEALVEREPVTVLCSAKGWIRAAKGHNVALAEIKYKDGDEERFVVPAAT
                     TDKLLIFATNGRFYTLPVDKLPGGRGHGEPLRLMIDLANEHEVAAILPYTPGAKLLLA
                     SSDGRGFVVPADEAVGQTRNGKAVMTPGEGVAVRVCVPAEGDSVAVIGSNRKLLIFPL
                     SEVPEMARGRGIILQRYHDGELSDAKVFTRADGLTWKLGDRVRTETDLRSWVAPRAGS
                     GRVAPQGFAKNNKFG"
     gene            2100..2330
                     /locus_tag="VGP50_12390"
     CDS             2100..2330
                     /locus_tag="VGP50_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEV8028223.1"
                     /translation="MSEPVILDTKGLKCPLPVLKARKAMRDLPAGGVLRVLATDPGAS
                     KDFAHFCKTTGHRLLASREEGGTLVFDIQKPE"
     gene            2361..3440
                     /locus_tag="VGP50_12395"
     CDS             2361..3440
                     /locus_tag="VGP50_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8028224.1"
                     /translation="MIALLMALRGAAMALQLLLSALLLRDARSVRAGRYGALFAGGAA
                     AAVVVWAPPFPIDPALWLIPLRVLAFGNTAVFWLLATALFDDEFEPSWVHAAAWLFLV
                     ALGLGGLYSGVSRPYLAFNALSLVLVFLALGQVLAGRALDLVESRRRLRVVLVVSVGL
                     FNTVVSVTAITVGGGGQPWFGLAEQLVSVMLSGFFSMMILSVTRTTPLLSLALMESRD
                     AAPRTVAARPPAAVPGDDDDEKLLASLRALMEKEKVYREEGLSIAILAGKLDLPEYRL
                     RRLINQRLGHRNFSTFLNGHRLADAMAALADPTQADVPILTIALDAGFQSIGPFNRAF
                     KAQAGMTPTEFRRQRRGAAAASLRS"
     gene            complement(3483..>4103)
                     /locus_tag="VGP50_12400"
     CDS             complement(3483..>4103)
                     /locus_tag="VGP50_12400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEV8028225.1"
                     /translation="HVVVRAESSIRSLRELKGKRVALGEPESGTLADARLVLEAAGLN
                     ECEIKAEYLRLTEATDALTQGRIDALFMVSGYPVPAITDVAATVPTRLLPIAPDVVDR
                     LVRKFSFMGADTIPGGSYPNIDEATPTVSTRALFIVRDDVKDDLVYAITKALWSDATR
                     RLLETAHPAGKRIKLANAFDGVAIPFHPGAARFYREAGMKLPADTQ"
BASE COUNT          635 a         1359 c         1428 g          681 t
ORIGIN      
        1 ctgaagccgg tgcatcgccg tgtgctctac gccatgcgcg agctcaacct cgacccgaaa
       61 tcgggctaca agaaatgctc gcgcgtcgtc ggcgacgtcg tcggcaaatt ccacccgcat
      121 ggcaacgagg ccgtctacga gaccttggtg cggctcgcgc aggatttcgc gcagcgctat
      181 ccgctggtcg acggccaggg caatttcggc aacatcgacg gcgacaatgc ggcggccatg
      241 cgatacaccg agagccgcct caccgaggtt gccgaggcgc tgctcaccgg cattggtcag
      301 gacgccgtcg atttccgcga cacctatgac ggcgagaaca gcgagccgac ggtgctgccg
      361 gcgcgcttcc cgaacctcct cgccaatggt gcgagcggca tcgccgtcgg catggccacc
      421 agcataccgc cgcacaatgc cggcgagctc tgcgacgcgc tcgctcacct cgtcaagcac
      481 aagcgggcga cgatcgagga gctcctggcg ctggtgcctg gccctgattt cccgacgggc
      541 ggcgtgctgg tcgagaacgc ggatgtcttc cgtcaggcct atgagaccgg gcgcggcagt
      601 ttccgcctgc gcgcgcgctg ggaggtggag aagctcgccc acggcgtcta tcagatcgtc
      661 gtcaccgaga tgccctatca ggtgccgaag gcgcgcctca tcgagaagat cgcggcgctg
      721 ctcgaggagc ggaagctgcc gctcctggcg gatctccgcg acgaatcgac cgacaaggtg
      781 cgtctcgtgc tggagcccaa gacgcgcaac gtcgacccgg gcgtgctcat ggagtcgctc
      841 ttccgcgcga cggagctcga gacgcgcatc agcctcaaca tgaacgtgct cgatgccgcg
      901 ggcgtgccgc gggtgatgaa cctccgggag gtgctgcagg ctttcctcga tcaccgccgc
      961 accgtgctgc agcgggtgac gcgcttccgc ctcgcggaga tcgcgcggcg cagcgagatc
     1021 ctcaaagggc agatgatcgt ctacctcaat ctcgacgagg tgatccgcat catccgcgag
     1081 catgacgacg ccaaggagcg catgatgaag cgctggcgtc tcagcgacgt gcaggccgag
     1141 gcgatcctca acatgcggct gcgcgccttg cgcaagctcg aggagatcga gatcaagaag
     1201 gagctgaaga cgctcggcga ggaacagaag gatttgagcg gcctgctcga ggacgacaag
     1261 cggcagtgga aggcgatcgc ggccgagctt gccgacatca agaaggcctt cggcgggggc
     1321 gcgctcggca agcgccggac cgagatcgct gccgcgcccg cgccggtgca ggtgccggtc
     1381 gaggcgctgg tcgagcgcga gccggtgacc gtgctgtgct cggccaaggg ctggatccgc
     1441 gccgcgaagg gccacaacgt cgcgctcgcc gagatcaagt acaaggacgg cgacgaggag
     1501 cgcttcgtgg tgcccgcggc cacgaccgac aagctcctga tcttcgcgac gaacggccgc
     1561 ttctacacgc tcccggtgga caagctgccg ggtgggcgcg gccatggcga gccgctccgg
     1621 ctgatgatcg atctcgccaa cgagcacgag gtcgcggcga tcctgcctta tacgcccggc
     1681 gccaagctcc tgctggcgtc gagcgacggg cgcggcttcg tcgtgccggc cgacgaggcg
     1741 gtcgggcaga cgcgcaacgg caaggccgtg atgacgccgg gcgagggcgt cgcggtgcgc
     1801 gtctgcgtgc cggccgaggg cgattccgtc gccgtgatcg gcagcaatcg caagctcttg
     1861 atcttcccgc tgagcgaggt gccggaaatg gcgcgcggcc gcggcatcat cctgcagcgc
     1921 tatcacgacg gcgaattgtc ggatgccaag gtgttcacgc gggctgatgg cctcacctgg
     1981 aagctcggcg accgcgtccg caccgagacg gatctccgca gctgggttgc gccgcgcgcc
     2041 ggcagcggcc gcgtggcgcc gcagggcttc gccaagaaca acaagttcgg ctgagtgtca
     2101 tgagcgagcc ggtgatcctc gatacgaaag ggctcaaatg cccgcttccg gtcttgaagg
     2161 cgcgcaaggc gatgcgcgac ctgccggcgg ggggcgtgct gcgcgtgctc gctaccgacc
     2221 ccggcgcgag caaggatttc gcgcatttct gcaagaccac gggacatcgg cttcttgcct
     2281 cgcgcgagga gggcggtacg ctggttttcg atatccagaa gcccgaatag ctgccgcgag
     2341 gccggcaaga aggacgagcg atgatcgctc tcttgatggc tctgcgcggc gccgccatgg
     2401 ccctgcaact gctgctgtcg gcgttgctgc tgcgcgatgc gcgcagcgtc cgggcgggcc
     2461 gctatggcgc gctcttcgcg ggcggagcgg ccgcggccgt cgtcgtctgg gcgccgccct
     2521 ttccgatcga tccggcgctt tggctgatcc cgctgcgtgt cctcgccttc ggcaacaccg
     2581 ccgtcttctg gcttctggca acggcgttgt tcgacgacga gttcgagccg tcatgggtcc
     2641 atgccgcagc ttggctcttt ctcgtcgcgc tcggtttggg cggtctttat agcggtgttt
     2701 cgcggccata cctggccttc aacgcgctgt ccctggtgct ggtattcctg gcgctggggc
     2761 aggtgctggc cgggcgcgcg ctggacctgg tcgagagtcg ccggcggctg cgcgtcgtcc
     2821 tcgtcgtgtc ggtcggactg ttcaacaccg tggtcagcgt caccgcgatc accgtcggcg
     2881 gcggcggcca accgtggttc ggcctcgcgg agcagctcgt ctcggtcatg ttgtccggct
     2941 ttttctccat gatgatcctg tcggtcaccc gcacgacacc gctcttatcg ctggcgctga
     3001 tggagagccg cgacgcggcg ccgcggacgg tcgccgcgcg ccctcccgcc gccgtgcccg
     3061 gcgatgacga cgacgagaag ctgctggcgt cgcttcgcgc gctgatggag aaggagaagg
     3121 tctatcgcga ggaggggctc agcatcgcca tcctggcggg caagctcgat cttcccgagt
     3181 atcgcctgcg gcggctcatc aatcagcgcc tcggccatcg caatttcagc accttcctca
     3241 acgggcaccg cctcgccgac gccatggcgg cgctcgcgga tccgacgcag gcggacgtgc
     3301 cgatcctcac catcgctctc gacgccggct tccagtcgat cgggccgttc aaccgtgcgt
     3361 tcaaggccca ggccgggatg acgccgaccg agttccgccg tcagcgccgc ggcgccgccg
     3421 cggcgagctt gcgcagctaa ttagttcggc ggcgaggggc gcctgctcgc gcccgatgcg
     3481 aattattgcg tgtcggccgg cagcttcatc ccggcctcgc ggtagaagcg cgcggcgccg
     3541 gggtggaagg gaatggcgac gccgtcgaag gcgttggcga gcttgatgcg cttgcccgcc
     3601 ggatgcgccg tctcgaggag gcgccgcgtg gcgtcgctcc acagcgcctt ggtgatggcg
     3661 tagacgagat cgtccttgac gtcatcgcgg acgatgaaga gcgcccgggt gctgaccgtg
     3721 ggggtcgcct cgtcgatgtt ggggtagctg ccgcccggaa tcgtgtcggc gcccatgaag
     3781 gagaatttgc ggacgaggcg gtccaccacg tcgggcgcga tcggcaagag ccgggtcggc
     3841 accgttgcag caacgtcggt gatggccggc acgggatagc cgctcaccat gaagagggcg
     3901 tcgatgcggc cctgcgtcaa cgcatcggtc gcttccgtca agcgaagata ctcggccttt
     3961 atctcgcatt cgttcaggcc ggccgcctcg agcacgaggc gcgcgtcggc gagcgtcccg
     4021 gattcgggct cgcccagcgc cacgcgcttg cccttgagct cgcgcaggct ccggatcgag
     4081 ctttccgccc gcaccacgac atg
//
LOCUS       DASYNS010000399         4227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_410667, whole genome shotgun sequence.
ACCESSION   DASYNS010000399 DASYNS010000000
VERSION     DASYNS010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410667"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VGP50_12405"
     CDS             complement(<1..828)
                     /locus_tag="VGP50_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV8028226.1"
                     /translation="MAREATIVADTYGSDEPGGEPPLIGDLGRRLLFTLGALVIYRLG
                     TYIPLPGIDPHIFQEIFSRNAGGILGPIDMFSGGALGRMTIFAISITPYISASFILQL
                     AIVVSKKLQALKNGGAAGRRTLNQYARIGALFLAAVQAYAMAIGLEGLGGGRAAVTDP
                     GLVFRLSTVVTLTAGTAPLIWLCEQITERGVGNGLALIIFSGIAARMPNAVGQLLEQG
                     RVGSMSYLEIDALLVGAVAVIAFVVFMERAQRRIRVHYPGRQIGQRTFSGKSSYLPLK
                     "
     gene            complement(818..1465)
                     /locus_tag="VGP50_12410"
     CDS             complement(818..1465)
                     /locus_tag="VGP50_12410"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV8028227.1"
                     /translation="MNLILLGPPGAGKGTQAKRLEQKYGLVQLSTGDMLRAECKSGSE
                     LGCKAQEIMDRGELVPDDIMIALIATCIDRHAGQGFILDGFPRTVPQAVALDDMLAKK
                     GLKLDRVVELTVDEAALIDRLAGRFTCAKCGTSYHDRYRRTKVQGVCDVCGSHDFIRR
                     ADDKPEAVKTRLVAYRKQTAPILPYYRQKGILRQVDGMADMDDVTHAIETVIRGP"
     gene            complement(1462..2805)
                     /gene="secY"
                     /locus_tag="VGP50_12415"
     CDS             complement(1462..2805)
                     /gene="secY"
                     /locus_tag="VGP50_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV8028228.1"
                     /translation="MASAAEQLAANINFSAFGKATELKNRIWFTLGALVIYRLGTYIP
                     LPGIDPHIFQDIFSRNAGGILGMIDMFSGGALGRMTIFALNIMPYISASIIVQLMTAV
                     SPTLEALKKEGEAGRKKLNQYTRMGTVLLAAVQAYGMAVGLEGMGGGRSAVIDPGIFF
                     RLSTVVTLAGGTVFLMWLGEQITARGVGNGISLIIFSGIVARIPNALGQLLEQGRVGS
                     MSYIVIILLLVGAVAVITFIVFMERAQRRILVQYPKRQVGNKMFGGEASHLPLKLNTS
                     GVIPPIFASSLLLLPATIAGFGASSSGGGWLAEITSYIGRGHPVYLLLYVALIIFFCF
                     FYTAIVFNPTETADNLRKYGGFVPGIRPGKNTADYLDYVLTRLTVVGALYLSAVCVLP
                     EILNSQLGLTFYFGGTSLLIVVSVTMDTVAQIHSHLLAHQYEGLIKKAKLRGRRG"
     gene            complement(2844..3323)
                     /gene="rplO"
                     /locus_tag="VGP50_12420"
     CDS             complement(2844..3323)
                     /gene="rplO"
                     /locus_tag="VGP50_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV8028229.1"
                     /translation="MKLNEIRDNKGAHYRAKRVGRGIGSGKGKTSARGGKGQTARSGV
                     ALNGFEGGQTPLHRRMPKRGFSHAAFRSAFVVVNLGRLQRALDDGRLKPDVKVDGAAL
                     VEAGVLRRVRDGVRLLAKGELKAPLTIEVTGASKAAIAAVEKLGGKVVVKGASPAAA"
     gene            complement(3491..3658)
                     /gene="rpmD"
                     /locus_tag="VGP50_12425"
     CDS             complement(3491..3658)
                     /gene="rpmD"
                     /locus_tag="VGP50_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV8028230.1"
                     /translation="MTQIASPIGREAYQRATLRGLGLDKLRRSRVLEDTPAVRGMVER
                     VKHLVRVEAAG"
     gene            complement(4013..>4227)
                     /locus_tag="VGP50_12430"
     CDS             complement(4013..>4227)
                     /locus_tag="VGP50_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV8028231.1"
                     /translation="AGGPMRAIFEALGVHDIVAKSVGTSNPHNMIKATFQALSRSVNP
                     RSVAARRGRKVSEILGRRDPAAEVRE"
BASE COUNT          691 a         1412 c         1397 g          727 t
ORIGIN      
        1 cttcagcggc aggtacgagg acttcccgct gaacgtcctc tgaccgatct gtcgtccggg
       61 atagtgcacg cggatgcgtc gctgcgcccg ctccatgaac accacgaagg cgatgaccgc
      121 gaccgcgccc accagcagcg cgtctatctc gagataggac atcgagccga cacgcccttg
      181 ctccagcaac tggcccacgg cattgggcat gcgcgccgcg atgcccgaga agatgatcag
      241 ggcgaggccg ttgcccacgc cccgctcggt gatctgctcg cagagccaga tcaggggcgc
      301 cgtgccggcc gtcagcgtga caaccgtgct gaggcgaaaa acgagcccgg gatcagtcac
      361 cgccgcccgc ccgccgccca gcccttcgag gccgatggcc atggcatagg cctgaacggc
      421 cgcgaggaac agcgcgccga tccgcgcata ctggttgagc gtcctgcgcc cggccgcgcc
      481 gccattcttc agcgcctgga gcttcttgga cacgacgatc gcgagctgca ggatgaacga
      541 ggcggagatg tagggcgtga tgctgatggc gaagatggtc atgcgcccga gcgcgccacc
      601 cgagaacatg tcgatcgggc cgaggatgcc gccggcgttg cggctgaaga tctcctggaa
      661 gatatgcggg tcgatgcccg gcagggggat gtaggtaccg agccgataga tcaccaaggc
      721 gcccagcgtg aacaagagac gccggcccag atcccctatg agtggcggct ctccgccagg
      781 ctcgtccgag ccgtatgtat cggcgacgat tgtggcctca cgggccacgg atgaccgttt
      841 cgatcgcgtg ggtgacgtcg tccatatcgg ccatgccgtc gacctgccgc aggatgcctt
      901 tctgccggta ataggggagg atcggcgcgg tctgcttgcg gtaggccacg agcctggttt
      961 tcaccgcctc cggcttgtcg tcggcgcggc ggatgaaatc atgcgagccg cagacgtcgc
     1021 acacgccttg gaccttggtc cgacgatagc ggtcgtgata gctggtgccg catttggcgc
     1081 aagtgaagcg gccggcgagg cggtcgatga gcgccgcctc gtcgacggtg agctcgacga
     1141 cgcgatcgag cttcagccct ttcttcgcca gcatgtcgtc gagcgccacc gcctgcggca
     1201 cggtgcgcgg gaaaccgtcg agaatgaaac cttggcccgc atgccggtcg atgcaggtgg
     1261 cgatcagcgc gatcatgatg tcgtcgggga cgagttcgcc ccgatccatg atctcctgcg
     1321 ccttgcagcc gagctcgctc cccgatttgc actcggcgcg cagcatatcg ccggtcgaga
     1381 gctggacgag cccgtatttc tgctcgagcc gcttcgcctg agtgccctta ccggcgccgg
     1441 gcggccccag caggatgagg ttcacccacg tctcccgcgc agcttcgcct tcttgatcag
     1501 accttcatac tgatgcgcca ggagatgcga atggatctgc gccacggtgt ccatggtgac
     1561 gctgacgacg atgagaaggc tggtgccgcc gaaatagaag gtgagaccca gttgcgaatt
     1621 gaggatctcc ggcagcacgc agacggcgga aaggtagagc gcgccgacca cggtcaagcg
     1681 ggtcagcacg tagtcgaggt agtccgccgt gttcttgccg ggccggatgc cgggaacgaa
     1741 gccgccgtat tttcgcaggt tgtcggcggt ctccgtcgga ttgaagacga tcgccgtgta
     1801 gaagaagcag aagaagatga tgagggcgac atagagcagc agatagaccg gatggccgcg
     1861 cccgatatag ctcgtgatct cggcgagcca gccgccgccg ctgctggacg cgccgaagcc
     1921 ggcgatggtc gccggcagca gcagcagcga ggacgcgaag atcggcggga tcacgcccga
     1981 ggtgttgagc ttcagcggca gatgcgaggc ttcgccgccg aacatcttgt tgccgacctg
     2041 gcgctttgga tactgcacca ggatccgccg ctgcgcccgc tccatgaaca cgatgaaggt
     2101 gatgaccgcg accgcgccca ccagcagcag gatgatcacg atataagaca tcgagccgac
     2161 gcgcccctgc tccagcagct ggccgagggc attgggtatc cgcgcgacga tgcccgagaa
     2221 gatgatgagc gagatgccgt tgccgacgcc ccgcgccgtg atctgctcgc cgagccacat
     2281 gaggaagacc gtgccgccgg cgagcgtgac gacggtcgag aggcggaaga aaatgcccgg
     2341 gtcgatgacc gcggaacgac cgccgcccat cccttcgagg cccaccgcca tgccataggc
     2401 ctggaccgca gccagcagca ccgtacccat gcgcgtgtac tggttgagct tcttgcgccc
     2461 ggcctcgcct tccttcttga gggcctcgag cgtcggcgag accgcggtca tcagctgcac
     2521 gatgatcgag gcggagatgt agggcatgat gttgagggcg aaaatcgtca tgcgcccgag
     2581 ggcgccgccc gagaacatgt cgatcatgcc gaggatgccg ccggcgttgc ggctgaagat
     2641 gtcctggaag atgtgcggat cgatgcccgg cagcgggatg taggtgccga ggcggtagat
     2701 gaccagcgcg cccaaggtga accagatccg gttcttgagc tcggtcgcct tgccgaaggc
     2761 ggagaaattg atattggccg cgagctgttc ggcggctgat gccatcttaa gccttcctcg
     2821 ggccccggcg ggccctcaag cgttcacgcc gcggcgggac ttgcgccctt gaccacgacc
     2881 ttgcccccca gcttttccac ggcggcaatc gccgctttcg atgctcccgt cacctcgatg
     2941 gtgagcggcg ccttgagctc gcccttggcg agtagccgga cgccgtccct gacacggcgc
     3001 agcacgccgg cctccaccag cgccgcgccg tccaccttca catcgggctt gagcctgccg
     3061 tcgtcaagcg cccgctgcaa ccgcccgaga ttgacgacga cgaaagcgga gcggaacgcc
     3121 gcatggctga agccgcgctt gggcatccgg cggtggagcg gtgtctggcc gccctcgaag
     3181 ccgttcagcg ccacgccgct gcgtgccgtc tggcccttgc cgccccgggc gctggtcttg
     3241 cccttgcccg agccgatgcc gcggccgacc cgtttggcgc ggtaatgggc gcccttgttg
     3301 tcgcgaattt cgttgagctt catcttcccg ctgtcctgaa gcagatctcg atcgagtgga
     3361 atcgacactt tttaacgtcg tcgtccccgc gaaagcgggg acccaggagc caccagtttg
     3421 ccgctcgccc tgggtccccg ctttcgcggg gacgacgacg aagaacggtt ccaggtagaa
     3481 cggacatgct ctaacccgcc gcctcgacac gcaccagatg cttcacccgc tccaccatgc
     3541 cgcggaccgc cggcgtgtcc tcgaggacgc gcgagcggcg cagcttatcg aggccgagcc
     3601 cccggagcgt ggcacgctga taggcctcgc ggccgatcgg gctggcgatc tgcgtcaccc
     3661 ggagcgtctt ctccggagcc gtcttgggct tcggcgcagc cttgggccgc ggcgctacgg
     3721 gcttggccgg tgcgggcgcg gcagccgccg cagccttccg cgcttcgcgc gccgccttct
     3781 tcgcggcctt cttcgcttcc ttcttggccg ctttgcgcgc gaccttcgcc gccgtttcgt
     3841 caggcgcggg cgcggccggt ttcgccttga ccttctcggg cgctgcgaca cccggtttgg
     3901 ccgccttcga cttctcgccc tgaggagaag cgggcttcgc tgccttcggc ttctcggcgg
     3961 cgtccgcctt cgccggtttc ttggccgcct tcttgtcgtc agccatcgcc ccttactccc
     4021 gtacttcggc tgcgggatcg cgccgcccca gaatctcgct caccttgcgg ccgcggcgcg
     4081 ccgcgacgct gcgcggattg accgagcggc tcaaggcctg gaaggtcgcc ttgatcatgt
     4141 tgtgcgggtt cgacgtgccg accgacttcg ccacgatgtc atgcacgccc agcgcctcga
     4201 agatggcgcg catcgggccg cccgcga
//
LOCUS       DASYNS010000400         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1035418, whole genome shotgun sequence.
ACCESSION   DASYNS010000400 DASYNS010000000
VERSION     DASYNS010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035418"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VGP50_12435"
     CDS             complement(<1..895)
                     /locus_tag="VGP50_12435"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV8028232.1"
                     /translation="MKRNNNFDALRLLAAVSVIFSHSFLLAEGRQDHEPLMRLTQGQS
                     ILGVVGVFVFFIISGFLVTESFETTASPLRFLAKRALRIYPGLILCILLCAFVLGPLA
                     TTLPLDAYFASGGLYDFLFSNLVLNVEHNGLPGVRFTGYDIGGIVDGPLWSLPCEVVM
                     YLMVLVLGSLRLLRLAVLLPLLALGLVCLRLDTAASPYFIGTVGWLLAFFVAGMILYK
                     LRDRRIFDGRIALVALAGLVVSIPLGRFLLLFPLFGAYLVLYLALDRRLPVLRAARFG
                     DLSYGLYIYGWPVQQGLLYASG"
     gene            complement(1003..1209)
                     /locus_tag="VGP50_12440"
     CDS             complement(1003..1209)
                     /locus_tag="VGP50_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 family protein"
                     /protein_id="HEV8028233.1"
                     /translation="MSGIRILGIVIAVVGAVVLVLGLNASHSLADQASQTFLGRYTHD
                     TTTYIIIGIAALVAGGLTALAARR"
     gene            complement(1293..1475)
                     /locus_tag="VGP50_12445"
     CDS             complement(1293..1475)
                     /locus_tag="VGP50_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028234.1"
                     /translation="MNDKQSAQNKAQQQPTQDQTAAPVATNEPAARREASERKPSKIE
                     CREWHGSGVCGDRHTD"
     gene            complement(1677..2141)
                     /locus_tag="VGP50_12450"
     CDS             complement(1677..2141)
                     /locus_tag="VGP50_12450"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEV8028235.1"
                     /translation="MTILVGTMTGTAQLVAEEVADALKARGVAAEVLPMDRLDAGVFA
                     RSPAFLICSSTYGQGDVPDNAQALFADLERAKPDLSRVAYGVIALGDRTYAQTYCFGG
                     KRFDGLLQSLGARRIGEPLLHDASAGAVPEEVAVEWIGPWIERFGAERDQAA"
     gene            2253..>2611
                     /locus_tag="VGP50_12455"
     CDS             2253..>2611
                     /locus_tag="VGP50_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8028236.1"
                     /translation="MATSEGADVRPENGEAAYLRLLGERIREARARRGMTRKLLARDS
                     SVSERYLAQLEAGQGNISIALLRQVAQAMGLPLADLVRDEPDRPVELTLLIQTLGRLS
                     SKELHEARRLLADAFGAA"
BASE COUNT          457 a          851 c          902 g          401 t
ORIGIN      
        1 cgccgctcgc atagagcagc ccctgctgca ccggccagcc atagatgtag aggccgtagg
       61 aaagatcgcc gaagcgcgcc gcgcgcagca cggggagccg ccggtcgagg gccagataaa
      121 gcaccagata ggcgccgaac agcgggaaca gcaggaggaa gcggccgagc gggatgctca
      181 cgaccagccc ggcgagcgcc acgagcgcga tgcgtccgtc gaagatccgc cggtcgcgaa
      241 gcttgtagag gatcatcccg gcgacgaaga aggcgagcag ccagccgacc gtgccgatga
      301 aataggggct cgccgccgtg tccagccgga ggcagacgag gcccagcgcc agcagcggca
      361 agagcacggc gaggcgcagc agccgcaagc tgccgaggac gagcaccatc agatacatca
      421 cgacttcgca gggcaggctc cacagcggcc cgtcgacgat gccaccgatg tcgtagccgg
      481 tgaagcgtac gccgggcaga ccgttgtgct cgacattgag cacgaggttc gagaagagga
      541 agtcgtagag cccgccgctg gcgaaatagg cgtcgagcgg cagcgttgtc gcgagcggcc
      601 ccagcacgaa ggcgcagagc aggatgcaca ggatgaggcc gggatagatg cggagcgcgc
      661 gcttcgccag gaaacggagc ggcgacgctg tcgtctcgaa gctctccgtg acgagaaagc
      721 cgctgatgat gaagaacacg aagacgccga cgacgccgag gatgctctgt ccctgcgtca
      781 gccgcatcag cggctcatgg tcctgacggc cctcggccag cagaaaggaa tgcgagaaga
      841 tcaccgagac cgcggcaagg aggcgcagcg cgtcgaagtt gttgttccgc ttcatggccc
      901 ttcgcggtta gtggccgtcg atcttatcga caagccgcac cgcgaagaag cgctaccggc
      961 gcagccgccg gagcgctgac tcggacggac gcttctttcc gactaccggc gtgcggcgag
     1021 agccgtcagg ccgcccgcga ccagcgcggc gatgccgatg atgatatagg tcgtcgtgtc
     1081 gtgggtgtat cggccgagga aggtctgcga cgcctggtcc gccagggaat gcgaggcgtt
     1141 gagcccgagc acgagcacga cggcgccgac caccgcgatc acgataccga ggatccgaat
     1201 gccgctcatc tctcgctccc gtctgcttcc aggggcaaac aacgcagccc ttcggcctgt
     1261 tccgtccggc gcctgggccg tgccgccttc gctcagtcgg tgtggcgatc gccgcagacg
     1321 cctgagccgt gccattcgcg gcactcgatc ttgctgggct tgcgctcgct cgcctcgcgg
     1381 cgggcggctg gctcgttcgt ggcgaccggc gcggccgtct gatcctgcgt cggctgttgc
     1441 tgcgccttgt tctgtgcgct ctgcttatcg ttcatgcgtt cctcctcgat cgaggcgttg
     1501 gatctgccgc ccatccctta tcgggtcttt gtcaacgatg ggccggccgt gtcggcacca
     1561 tcgaacgtcc ttagggttgc ggctgctgcg ctgcgccttg cccgggaata cgatggtccg
     1621 cgtcttcgca agcatcgggc gaaccgggtt aaggtctgat taacctgcgg ttgtgtctac
     1681 gcggcctggt cgcgctcggc gccgaagcgt tcgatccagg ggccgatcca ttcgacggcg
     1741 acctcttccg gcacggcgcc ggcggaggcg tcatgcagca gcggctcgcc gatgcgccgc
     1801 gcgcccaggg actgcagcag cccgtcgaag cgcttgccac cgaagcagta ggtctgggca
     1861 taggtgcggt cgcccagcgc gatcacgcca taggcgaccc gcgacaggtc gggcttggcg
     1921 cgctcgagat cggcgaacag cgcctgcgca ttgtcgggaa cgtcgccctg gccgtaggtc
     1981 gagctgcaga tcagaaaggc ggggctgcgg gcaaagacac cggcgtcgag gcggtccatc
     2041 ggcagcacct cggccgcgac gccgcgcgcc ttcagggcgt ccgccacttc ctcggccacg
     2101 agctgcgcgg tgccggtcat ggtcccgacc agaatggtca agggttgatc caacgcggga
     2161 acctccttcc ggcctggacg gcccggtgga agagactata atgcatgtta tttcctaagc
     2221 aagcattata gtacatacgg tgtggagcgc agatggcgac cagcgaggga gcagatgtcc
     2281 ggccggagaa tggcgaggcc gcctatctcc gcttgctggg cgagcgcatc cgcgaggcgc
     2341 gggcgcgccg cggcatgacg cgcaagctgc tggcgcgcga ttccagcgtc tccgagcgct
     2401 atctggcgca gctcgaagcg gggcaaggca atatctcgat cgcgcttctg cgccaggtcg
     2461 cgcaggccat ggggttgccg ctcgccgatc tcgtgcgcga cgagccggac cggccggtcg
     2521 aactcacgct cctcatccag accctcggcc ggctttcgtc caaggagctg catgaggcgc
     2581 gccgcctcct cgccgacgcc ttcggcgccg c
//
LOCUS       DASYNS010000401         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_100129, whole genome shotgun sequence.
ACCESSION   DASYNS010000401 DASYNS010000000
VERSION     DASYNS010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100129"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VGP50_12460"
     CDS             complement(<1..324)
                     /locus_tag="VGP50_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEV8028237.1"
                     /translation="MPSSAASEPIAPLPTLDDITGAARRLDGHAVLTPLLESAAINAR
                     LGGRLLVKAEPLQRTGSFKFRGAFNALAQLEPQQRRAGVVAYSSGNHAQGVAAAAQAL
                     GIRAAI"
     gene            complement(302..1153)
                     /locus_tag="VGP50_12465"
     CDS             complement(302..1153)
                     /locus_tag="VGP50_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8028238.1"
                     /translation="MTSFRERRLTTQDGLSLYFRDYGDPLSPRLPLLCLAGLVRNSAD
                     FADLAERHAGERRIVCLDYRGRGRSDYDADWRNYHPMTYVRDIAHLLAALDLHRVAIV
                     GTSFGGLLTMGLAALKPTSLAAVVLNDIGPDLVDGGFSRIVNYVGVDQPQPDWESAVS
                     YLQQLLPQMSIRSELGWQKFARATYRSGKDGLLHFDWDVAAAKPLTMQRSEFPDLWPM
                     FRALRPVPTLVCRGASSDVLSTETFERMALAKPDLLRVTVQGSGHTPTLNEPDAAAAV
                     DAFIRRF"
     gene            complement(1173..>2586)
                     /locus_tag="VGP50_12470"
     CDS             complement(1173..>2586)
                     /locus_tag="VGP50_12470"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEV8028239.1"
                     /translation="KRLDRLLWDDQTEAVHRMMPRVDADWRALADARIRMADFSRGAD
                     QALARVPASLQNDPGLLFEHMRWLNRRGHQDQAIEILGQVTDDPVRPVAWATERQSLS
                     RYALATGDLALAYRIAAKHRLTSGPVYAELEFFAGWVALRFQHQSEIAYNHFVGLYEV
                     VKLPVSVARASYWAARAADAMGYNQLAATWYRTAAEHVTTYYGQLAAAALSEPTLVHA
                     LEEPTPTAADTDAFEKHELVQVLRDLADAGASEYMRPFVLRLSELAKTPGEHALITHL
                     ALKIERPDLAITAAKKASYAGVVLLAEGYPLTELPPGGNVEHPLVLAMTRQESAFDRE
                     AVSSAGALGLMQLMPKTAKTVARALRLQFSKRRLTTDRSYNVTIGRAYLNGLLGDFSG
                     SYVLAICAYNAGPARVRQWIRDYGDPRSDKVDVIDWIESIPISETRNYVQRVLENLQL
                     YRLRMGDHGLAFSLVSDLRR"
BASE COUNT          432 a          809 c          945 g          400 t
ORIGIN      
        1 gatggcggcg cggatgccga gcgcctgcgc ggcggcggca acgccctggg cgtggttgcc
       61 cgaggaataa gcgacgacac ccgcgcggcg ctgctgcggt tcgagctgcg ccagcgcgtt
      121 gaaagcgccg cggaacttga aggagccagt ccgctgtaaa ggctccgcct tgacgaggag
      181 gcggccgccg agccttgcgt tgatcgccgc ggattcgagc aacggcgtca gaacggcgtg
      241 gccgtcgagc cgtcgcgccg cgccggtgat gtcgtcgagt gtggggagcg gcgcgatcgg
      301 ctcagaagcg gcggatgaag gcatcgacgg cagcggcggc gtccggttcg ttcaaggtcg
      361 gcgtatggcc cgacccctgc accgtaacac gcaacaggtc gggcttcgca agcgccatgc
      421 gctcgaaggt ctcagtcgag agcacgtccg atgatgcgcc ccggcaaacc agcgtcggga
      481 ccggacggag cgcgcggaac atcggccaaa gatccgggaa ctcgctgcgc tgcatggtga
      541 gcggtttggc cgcggcaaca tcccaatcga aatgaaggag cccgtctttc ccggagcgat
      601 acgtcgcgcg agcgaatttc tgccatccca gctcgctcct gatagacatc tgcggcagca
      661 actgctgcaa atagctcacg gcgctctccc aatccggctg cggctgatcg acgccgacgt
      721 agttgacgat gcgcgagaaa ccgccatcga cgaggtcggg gccgatgtcg ttgagcacga
      781 ccgcggcaag cgaggtcggc ttgagcgcgg ccagacccat ggtcaggaga ccgccgaagg
      841 aggtgccgac gatcgcgacg cggtgcagat cgagcgcggc gaggagatgc gcgatgtccc
      901 gcacataggt catcggatgg tagttgcgcc agtccgcgtc gtaatccgag cggccgcggc
      961 cccgatagtc gaggcagacg atccgccgct cgcccgcgtg gcgctcggcg agatcggcga
     1021 agtccgcgct gttgcgtacc agtccggcaa ggcagaggag cggcaagcgc ggcgacaagg
     1081 gatcgccata gtcgcggaaa tagagcgaaa ggccgtcctg cgtggtgagc cgccgctcgc
     1141 gaaacgaggt catgggaaag ccggtgccgc cgtcagcgtc tcaggtcgct gacgagcgag
     1201 aaagcgaggc cgtggtcgcc catgcgcagg cggtagagct ggaggttctc gagcacgcgc
     1261 tgcacgtagt tgcgggtctc gctgatcgga atggactcga tccagtcgat gacgtcgact
     1321 ttgtcggagc gcgggtcgcc gtagtcgcgg atccactgcc ggacacgggc gggtccggca
     1381 ttgtaggcgc agatcgccag cacataggag cccgagaagt cgcccagcag accgttgaga
     1441 taggctctgc cgatggtgac gttgtagctc cggtcggtgg tgaggcggcg cttcgagaat
     1501 tggaggcgca gcgcgcgcgc cacggtcttt gccgtcttcg gcatgagctg catcaggccg
     1561 agcgcgccgg ccgagctcac ggcttcgcgg tcgaaagcgc tttcctggcg cgtcatcgcc
     1621 agcaccagcg ggtgttcgac attgccgccg ggcggcagtt cggtgagcgg atagccttcc
     1681 gccagcagga cgacgccggc atagctcgcc ttcttggcgg cggtgatggc gaggtcgggg
     1741 cgctcgatct tgagcgccag atgggtgatc agcgcatgct cgcccggcgt cttggcgagc
     1801 tcgctcaagc gcagcacgaa gggccgcata tactcgctgg cgcccgcgtc ggcgaggtcg
     1861 cgcagcactt ggaccagctc atgcttctcg aaggcgtcgg tgtccgccgc ggtgggcgtg
     1921 ggctcttcga gcgcatgcac gagcgtcggc tcgctgagcg cggcggcggc gagctggccg
     1981 taataggtcg tcacatgctc ggcggcggtg cggtaccagg tcgccgccag ctggttgtag
     2041 cccatggcgt cggcggcgcg ggcggcccag taggaggcgc gcgccacgct gaccggcagc
     2101 ttcacgactt cgtagaggcc gacgaagtgg ttgtacgcaa tttctgattg atgctggaag
     2161 cgcagtgcca cccagccggc gaagaactcg agctcggcat agaccggccc gctagtgagg
     2221 cggtgcttgg cggcgatgcg gtaggcgagc gcgagatcgc ccgtggccaa cgcatagcgc
     2281 gacaggctct gccgctccgt ggcccaggcg acgggccgca ccgggtcgtc ggtcacctgt
     2341 ccgaggatct cgatcgcctg atcctgatgt cccctgcggt tcagccagcg catatgctcg
     2401 aagagcaggc cgggatcgtt ctgcagcgat gccggcaccc gcgcgagcgc ctggtcggcg
     2461 ccgcgcgaga agtcggccat gcggatgcgc gcatcggcaa gcgcgcgcca gtcggcgtcg
     2521 acgcgcggca tcatgcggtg caccgcctcc gtctggtcgt cccagagcag gcgatcgagc
     2581 cgcttc
//
LOCUS       DASYNS010000402         6392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4158295, whole genome shotgun sequence.
ACCESSION   DASYNS010000402 DASYNS010000000
VERSION     DASYNS010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4158295"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGP50_12475"
     CDS             <1..312
                     /locus_tag="VGP50_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HEV8028240.1"
                     /translation="RMVEIQAAREAAQPIRARTGGSTFANPPGHKAWELIDRAGCRGL
                     KRGSAMVSEKHANFLINTGTATAADLETLGEDVRRRVRETSGVALEWEIRRIGRSGGA
                     A"
     gene            309..1226
                     /locus_tag="VGP50_12480"
     CDS             309..1226
                     /locus_tag="VGP50_12480"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV8028241.1"
                     /translation="MSRRVAVLMGGWSSERDVSLVSGRECAKALIARGYDAYTIDVTR
                     DLTALAAALTPRPDVVFNALHGRGGEDGTVQGVLELLQIPYTHSGVLASAVAMDKPTA
                     KAVFAAAGLPLVEGRVVTRAQLAAGDPIARPFVVKPTNEGSSVGVRIVREKDNSWGEE
                     ARSWAFELSLVERFIPGRELTVAVMGNRALGVCEIRAHGTFYDYTAKYAAGGSEHLVP
                     APIHSRAYEEALDIGLRAHQALGCRGVTRSDLRYDDTAGEPGRLYLLEINTQPGMTPT
                     SLVPDLARHVGIDFESLVEWIVENAACDS"
     gene            1214..2053
                     /locus_tag="VGP50_12485"
     CDS             1214..2053
                     /locus_tag="VGP50_12485"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEV8028242.1"
                     /translation="MRFLRRERETAQRRRPHAALRGRLVALGGAALALAGAGWWLHHT
                     GAIAHAVAAAGQHVLTLTAHCGLAVTDIEVEGRRRASREAVLDALGVASGTPILAIDA
                     TEAKRRLETIAWVRSASVERRLPDMLYVRLIERQPLALWQRQGKLVLVDHEGVVISGQ
                     RPEAFADLIVLVGNDAPANGAALIDMLQSEKSLAGHVAAAVRVGGRRWNLRFDSGIDV
                     ALPEEDAPGAWRRLAALEESDGILERNVEMIDLRLPDRLVLRTPEPQKPAGKRSRQPG
                     KAT"
     gene            2050..3300
                     /gene="ftsA"
                     /locus_tag="VGP50_12490"
     CDS             2050..3300
                     /gene="ftsA"
                     /locus_tag="VGP50_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV8028243.1"
                     /translation="MKRLAPRPRGTIGIVDIGTSKVCCFIARREDREPRIIGIGHQVS
                     RGVRNGIVVDIDAASQSILAAVHAAEQMAGETLGEVVANLSGGFAGSRIIKTEINLNG
                     REITDTDMRRVLDQGHLLKEPPEREVIHSIPVGFSVDQSRGIRDPRGMYGQRLGVNMH
                     LVTASAGAVRNLATCIGRCHLDIGAVVVSPYAAGLASLVEDETDLGVTVLDMGGGTTG
                     IAVFFDGNLVFADSVPVGGGHVTNDIARGLSTPIAHAERMKTLYGSAIASLADEREVI
                     NVPQVGEEDEGQANHVPKSLLIGIIAPRLEETFELVRDRLDASGFDKIAGRRVVLTGG
                     ASQLPGTRELAALILDKQIRGGRPLRIDGLAEAMAGPAFATSAGLVHFALSERAETPR
                     RGRALTEEPGGLFVRVGHWIKENF"
     gene            3371..4828
                     /gene="ftsZ"
                     /locus_tag="VGP50_12495"
     CDS             3371..4828
                     /gene="ftsZ"
                     /locus_tag="VGP50_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV8028244.1"
                     /translation="MSITVSIPEEQELKPRIAVVGVGGAGGNAVNNMIRSNLIGCEFL
                     VCNTDAQAMKQSLAERKIQLGASITRGLGAGSRPDVGRIAAEETLEEIMTALEGANMV
                     FITAGMGGGTGTGAAPVIARAAREAGILTIGVVTKPFQFEGSHRMRLAEAGIIELQKF
                     VDTLIIIPNQNLFRVANERTTFADAFKMADDVLHSGVRGVTDLMVMPGLINLDFADIR
                     AVMSEMGKAMMGTGEAEGDRRAIDAAEAAISNPLLEDVSMRGARGVLINITGGPDMTL
                     FEVDEAANRIREEVDPDANIIFGSTFDDKLAGRVRISVVATGIDAEVMAQPRPVIQLV
                     SDRGQPAATAADAPAPQVAIEAPPVPAAAQAIESAAVKSGAFIAPKPLETAAKPAHVA
                     QPAFAPDAYAAAAMENGGKAPTKPAKTRSPSLFERVTGVTRGRSTSEVAPLPRSAPPQ
                     QPAQPRLGPLDPADRLGAKEEDLLDIPAFLRRQAN"
     gene            complement(4923..5120)
                     /locus_tag="VGP50_12500"
     CDS             complement(4923..5120)
                     /locus_tag="VGP50_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028245.1"
                     /translation="MTEHRGDDATPASVVERLRESERRYRELLDAAAVESEVGMGSRF
                     DVYLPAAADSSKASASVEGAR"
     gene            complement(5141..>6392)
                     /locus_tag="VGP50_12505"
     CDS             complement(5141..>6392)
                     /locus_tag="VGP50_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028246.1"
                     /translation="SRKREDGTIQWIKTSGAPIFDEDGAFLGYRGVAANVTVQVEAVQ
                     AARLAQRQLHDAVTHVTQPLVLYDSEDHAVAFNQAFTDLYRAPQVTWPVMQGISFRAL
                     AEWQLSVGFYAEDKEQSAVSLDALLEQYESGRPHTYHLRDGRWMLVLYRRLPGGGRVG
                     LWTDVTDIKRAEAERRRLEAQLQHSQRLEALGTLAGGAAHEINNALVPVVALAKLMAA
                     KFPEDSRERRNLMTMLAGAERSRDLVKQILAFSRKDEQEDRRESVDIAAVLRDALRLM
                     RATVPTTIRLWDEIAAVPPVTGDANQLHQVIVNLVTNAAQAIGDAHGTITLGVRAEAD
                     GAALRLWVADTGCGMDEATKARIFEPFFTTKDVGEGTGLGLSVVHGIITEHGGRIEVE
                     SERGCGTRFDILLPLRAAKTGAAA"
BASE COUNT         1038 a         2222 c         2136 g          996 t
ORIGIN      
        1 cgcatggtcg agatccaggc agcgcgcgaa gcggcacagc cgatccgcgc gcgcaccggc
       61 ggctccactt tcgccaatcc gccgggccac aaggcctggg aactcatcga ccgcgccggc
      121 tgccgcgggc tcaagcgcgg cagcgccatg gtctccgaga agcatgccaa cttcctcatc
      181 aataccggga ccgccactgc cgccgatctc gagacgctgg gcgaggacgt gcggcgccgc
      241 gtccgtgaga cgagcggtgt cgcgctcgaa tgggagatcc gccgcatcgg ccgttccgga
      301 ggtgccgcat gagccgtcgc gtcgccgtgc tgatgggcgg gtggtcgtcg gagcgcgatg
      361 tgtctctcgt gagcggacgc gaatgcgcga aggctcttat cgcgcgcgga tatgacgcct
      421 acaccatcga cgtgacccgc gacctgaccg cgctcgcggc cgcgctgacg ccgcgtcccg
      481 atgtcgtctt caatgcgctt catggccgcg gcggagagga tggcacggtg caaggcgttc
      541 tcgagctctt gcaaattccc tacacacatt cgggcgtcct cgcctctgcc gtcgccatgg
      601 acaaaccgac ggcgaaagcc gtcttcgccg ccgccggact gccgctcgtc gaaggcaggg
      661 ttgtcacgcg cgcgcagctt gctgccggcg acccgatcgc caggcctttc gtcgtcaagc
      721 cgacgaacga aggctcgagc gtcggcgtgc gtatcgtgcg cgagaaggac aactcctggg
      781 gcgaagaggc gcgaagctgg gctttcgagc tgtcactggt ggagcgcttt attcccggac
      841 gcgagctcac cgtcgcggtc atgggtaatc gagcgcttgg cgtctgtgag atccgggcgc
      901 atgggacgtt ctacgactac acggcaaaat atgccgcagg cgggtcggag catctcgttc
      961 ccgcacccat ccattcgcgc gcctatgagg aagcgctcga tatcggtctg cgcgcgcatc
     1021 aggcgctcgg atgccgcggg gtgacccgca gcgacctgcg ctatgacgac acggcgggtg
     1081 agccgggccg cctctatctc ctcgaaatca atacacagcc gggcatgacg ccgacgtcgc
     1141 tggtgccgga ccttgcacgc cacgtcggca tcgacttcga gagcctcgtc gagtggatcg
     1201 tggagaacgc cgcatgcgat tcctgaggcg cgagcgcgaa accgcgcagc ggcggcggcc
     1261 gcacgccgct ttgcgcggcc gccttgtcgc tttgggcggg gccgcgctcg cgctcgccgg
     1321 tgccggttgg tggctgcatc acacgggcgc catcgcccat gccgttgcag cggcagggca
     1381 gcacgtgctg accctgaccg cgcattgcgg gctcgccgtt accgacatcg aggtcgaggg
     1441 acggcgccgg gcgagccgcg aggcggttct cgatgcgctg ggcgttgcga gcggtacgcc
     1501 gatcctggcc atcgacgcga ccgaggccaa gcgccggctc gagacgatcg cctgggtacg
     1561 ttcggcctcg gtcgagcgtc ggctccccga catgctctat gtgcggctca tcgagcgcca
     1621 gccgctggcg ctctggcaac gccagggcaa gctggtgctg gtcgaccatg agggcgtggt
     1681 gatctccggc cagcggcccg aagcttttgc cgatctcatc gtgcttgtcg gcaacgacgc
     1741 gccggccaat ggtgccgcgc tcatcgacat gctgcaaagc gagaagtcgc tcgccgggca
     1801 cgtcgctgcc gcggttcgcg tcggcggccg gcgctggaac ctgcgcttcg acagcggcat
     1861 cgacgtggcg cttcccgagg aggacgcgcc gggggcttgg cggcgcctcg ccgcgctcga
     1921 ggagagcgac ggcattctcg agcgcaatgt cgagatgatc gacctgagat tgcccgaccg
     1981 cctggtgctg cgcacgcccg agccgcagaa gccggccggc aaacggtcgc gacagccggg
     2041 gaaagcgaca tgaaacggct cgccccgcgc ccgcgcggca ccatcggcat cgtcgacatc
     2101 ggcacgagca aggtgtgctg cttcatcgcg cgccgcgaag atcgcgagcc gcggatcatc
     2161 ggcatcggcc atcaggtctc gcgcggcgtg cgcaatggca tcgtcgtcga catcgacgcc
     2221 gcgagccagt cgatcctcgc cgccgtgcat gcggccgagc agatggccgg tgagacgctg
     2281 ggcgaggtcg tcgcgaatct ttccggcggc ttcgccggct cgcgcatcat caagaccgag
     2341 atcaacttga atggccgcga gatcaccgac accgacatgc gccgggtgct cgaccagggc
     2401 catctgctca aagagccgcc cgagcgcgag gtcatccatt cgatcccggt cggcttctcg
     2461 gtcgaccaga gccgcggcat ccgcgatccc cgcggcatgt atggccagcg gcttggcgtc
     2521 aacatgcatc tggtgacggc ctcggccggt gcggtgcgca acctcgccac ttgcatcggc
     2581 cgctgccacc tcgacatcgg cgccgtcgtg gtgagccctt acgcggcggg gctcgcctct
     2641 ctcgtcgagg acgagaccga cctcggggtc acggtcctcg atatgggcgg agggacgacc
     2701 gggatcgccg tgttcttcga cggcaatctg gtcttcgccg acagcgtgcc ggtgggcggc
     2761 ggccacgtca ccaacgacat cgcgcgcggc ctctcgacgc cgatcgcgca tgccgagcgc
     2821 atgaagacgc tctatggcag cgccattgcc tctctcgccg acgagcgcga ggtcatcaac
     2881 gtgccgcagg tcggcgaaga ggatgaaggc caggcgaatc acgtgccgaa atcgctgctc
     2941 atcggcatca tcgcgccccg gctcgaggaa accttcgagc tggtgcgcga ccgcctcgac
     3001 gcctcgggct tcgacaagat cgccgggcgc cgcgtcgttc tcacgggcgg cgcctcgcag
     3061 ctgccgggaa cgcgtgagct ggcggcactc atcctcgaca agcagatccg cggcgggcgg
     3121 ccgttgcgga tcgacggtct ggcggaggcg atggcgggtc ccgccttcgc gacctcggcc
     3181 ggactcgtcc acttcgcgct ttccgagcgc gccgagacgc cgcggcgcgg ccgcgcgctc
     3241 acggaggagc cgggcggact tttcgtccgc gtcggtcatt ggatcaagga gaatttctga
     3301 tgctgaaaat cagaagcccg ataacggagg cggcatggca cagcaacgcg agaccttccc
     3361 ggaggatagg atgagcatca ccgtgagcat accggaagaa caggaattga agccgcgcat
     3421 cgccgtcgtc ggcgtcggcg gcgccggcgg caacgccgtc aacaacatga tccgctccaa
     3481 tctcatcggc tgcgagttcc tcgtctgcaa caccgacgcg caggcgatga agcagtcgct
     3541 ggccgagcgc aagatccagc tcggcgccag catcacccgc ggccttggcg ccggctcccg
     3601 gcccgatgtc ggccggatcg ccgccgagga gacgctcgaa gagatcatga ccgcgctcga
     3661 gggcgccaac atggtgttca tcaccgccgg catgggcggc ggcaccggca ccggcgcggc
     3721 gccggtcatc gcccgcgcgg cgcgcgaggc gggcattctg acgatcggcg tcgtcaccaa
     3781 gcccttccag ttcgagggct cgcaccgcat gcgccttgcc gaggccggca tcatcgagct
     3841 gcagaaattc gtcgacaccc tcatcatcat ccccaaccag aacctcttcc gcgtcgccaa
     3901 cgagcgcacg acattcgccg acgccttcaa gatggcggac gacgtgcttc attcgggcgt
     3961 gcgcggcgtc accgatctca tggtgatgcc gggtctcatc aatctcgatt tcgccgatat
     4021 ccgcgccgtg atgagcgaga tgggcaaggc gatgatgggc acgggcgagg ccgagggcga
     4081 ccgccgcgcc atcgacgccg ccgaggccgc gatttccaac ccgctgctcg aggacgtctc
     4141 gatgaggggc gcccgcggcg tcctcatcaa catcaccggc gggccggaca tgacgctctt
     4201 cgaggtcgac gaggccgcga accgcatccg cgaggaggtc gatcccgatg ccaacatcat
     4261 cttcggctcg accttcgacg acaagctcgc gggacgcgtg cgcatctcgg tggtcgcgac
     4321 cggcatcgat gccgaggtca tggcgcagcc caggcccgtg atccaactgg tgagcgaccg
     4381 cggtcagccg gcggcaaccg ccgccgacgc gcccgcgccg caggtcgcca tcgaagcacc
     4441 cccggtgccc gcagccgcgc aggccattga gagcgcggcg gtcaagagcg gcgccttcat
     4501 cgcgccaaag ccgctcgaga cggcggcaaa acccgcgcat gtggcacagc cggccttcgc
     4561 tcccgatgcc tatgccgccg cagccatgga gaatggcggg aaggctccga cgaagccggc
     4621 gaagacgcgt agtccgagcc tcttcgagcg cgtgaccggc gtgacgcgcg gccgcagcac
     4681 cagcgaagtc gcgccgctgc cgcggagcgc gccgccgcaa cagcccgcgc agccgcgcct
     4741 cgggccgctc gatccggccg accggctcgg cgccaaggag gaggatctcc tggacattcc
     4801 ggccttcctg cggcgccaag cgaattgatg ccggtgccca tcgacgccct gcgcgaatcg
     4861 tcgccgccag ggcggccgat ggggatcatc gacgccggca cgagtgccgc agcacggtga
     4921 tgtcagcggg cgccttcgac cgaagcgctc gcctttgacg agtcggccgc tgccggaaga
     4981 tagacgtcga agcgcgaacc catgccgact tcgctctcga cggccgcggc gtcgagcagt
     5041 tcccgatacc gccgttcgct ctcgcgcaag cgctcgacca cgcttgctgg cgtcgcgtcg
     5101 tccccgcgat gttcggtcat tttggccatc atttcgtcgc tcaagcggcc gcgccggttt
     5161 tcgccgcccg tagcggcaac aggatgtcga agcgcgtgcc gcagccgcgc tcgctctcca
     5221 cctcgatgcg gccgccatgc tcggtaatga tgccatgcac caccgaaagc ccgaggcccg
     5281 tgccctcgcc gacgtctttc gtcgtgaaaa acggctcaaa gatgcgcgct ttcgtcgcct
     5341 cgtccatgcc gcagcccgta tcggccaccc agagacgcag tgccgcacca tcggcttcgg
     5401 cgcgcacgcc cagcgtgatc gtgccatgcg cgtcgccgat tgcctgcgcc gcgttggtca
     5461 ccagattcac gatcacctga tgaagctggt tcgcatcgcc cgtcaccggc ggcacggcgg
     5521 cgatctcgtc ccaaagacgg atggtggtcg ggacggtcgc acgcatcagg cgcagcgcgt
     5581 cccgcagtac ggcggcaata tcgacgctct cgcgtcgatc ctcctgctcg tccttgcggc
     5641 tgaatgcaag gatctgcttc actaggtcgc gggagcgctc ggcaccggcg agcatggtca
     5701 tgaggttgcg ccgctcgcgg ctgtcctcgg gaaacttggc cgccatgagc ttggcgagcg
     5761 cgaccaccgg cacgagcgca ttgttgatct cgtgggcggc gccgcccgcc aacgtgccca
     5821 gcgcctcgag ccgctgcgag tgctgcagtt gcgcctcgag gcggcgccgc tccgcctccg
     5881 cgcgcttgat gtcggtgaca tccgtccaaa gtccgacgcg gcccccgccg ggcaggcgac
     5941 ggtagaggac cagcatccag cggccgtcgc ggagatgata ggtatgcgga cgcccgctct
     6001 cgtattgctc gagcaacgca tcgagcgaga cggccgactg ctccttgtcc tccgcgtaga
     6061 atccgacgct cagctgccac tcggcgagcg cgcgaaacga gatcccctgc attaccggcc
     6121 atgtcacctg gggagcgcga tagaggtcgg tgaatgcctg gttgaaggca accgcgtggt
     6181 cttccgagtc gtagaggacg agcggctgcg tcacatgcgt aacggcatca tgcaactgac
     6241 gctgagccag gcgcgccgcc tgaacggcct ccacctgcac cgtgacattg gcggccaccc
     6301 cgcgataccc gaggaacgcg ccatcctcgt cgaaaatcgg cgcgccgctc gtttttatcc
     6361 attgtatcgt gccgtcctcg cgtttccggg aa
//
LOCUS       DASYNS010000403         7859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1348187, whole genome shotgun sequence.
ACCESSION   DASYNS010000403 DASYNS010000000
VERSION     DASYNS010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348187"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1433
                     /locus_tag="VGP50_12510"
     CDS             <1..1433
                     /locus_tag="VGP50_12510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028247.1"
                     /translation="AHAMIREIDGRIRFWSSSLERLYGWKREDAIGQISHDLLSTEFP
                     KPPAEIEAELLATRRWEGELRHRSRDGRTVIVASHWALDRKDLGQPAWVIEVNNDITA
                     LKAAEEERNRFNAALGEAHQRLGLAIDAGQIGTWELELRTGRMRHSARRAALMGLPAL
                     AVEEHYEEFLARVHPDDRQMVARMLSASVEDGREFRGTFRFVWPDGSIHWVESQAAPP
                     RDRSADPVRIMGASIDITERMLREAELRRAKEAAERATQSKSRMLAAVGHDLKQPLHI
                     IGMALSMLQRRVADLDPKRLLIRADRAVDQLAAALDTLLEVAQLEAGVVAPRIEAFPV
                     ERILNDIEDQFRLLAAHKGLSFTVEHSSALVRSDPKMLATILHNLVGNAIKYTEEGRI
                     DVACRQHDAVLSIEVRDTGIGIPPDKLELVFEEFQRLETSSSKGGLGLGLAIVRHTAN
                     VLGHPLRVQSNVGQGSVFSVDIPAGS"
     gene            complement(1671..2735)
                     /locus_tag="VGP50_12515"
     CDS             complement(1671..2735)
                     /locus_tag="VGP50_12515"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV8028248.1"
                     /translation="MKNLAFSRAVGALAGLASLAWAAQCHAVEPGDTDPHQPGAAAGA
                     PLAALPPPGFYFVNNFNYVSGSLDDGNGNVNKAPAHTAVNVTAYVEIPVLTWSSPFHI
                     LGAQYGATLLQPIVTNSATTTSVSPITHSLTSVTTNKTGLDNTAFSPLVLSWHLPAGF
                     FAAISTPIAIDDGDNEAHAKGGTSVTPHNAADYWIFSPSAYLAWFNGHGTQLSVNAEY
                     DIQTKDNNFLNVGGSQISYQSGDFLTLDFGALQTLPGVYHKWSVGIVGSFATQTTDDT
                     LSGNPAALAAVGAAPGRVPANFLNLGNSAGNRFQSLSLGPSVFYEFGPIVMNLYYTRD
                     LFAKNTASTSTVWFAFAIPL"
     gene            complement(3081..4235)
                     /locus_tag="VGP50_12520"
     CDS             complement(3081..4235)
                     /locus_tag="VGP50_12520"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV8028249.1"
                     /translation="MTYTAGRIVYDADSHVMETREWLEPFMDDDLKKKLRPLYSRDPG
                     RIAKLLEQAKARKGDKDAERAAFENPIGGPKGWLAAGAFDPAERSRVLDVFGFSAQLV
                     FGTFSLAPALEAKDEGLMYAGVRARNEAMAAFCRNDKRLFGVAYAPLDNPKRALEEVD
                     AALRIGSRAIMVSAGPAGERSPGHPDLDPLWARLSETDTPFILHIGPGSLNQPAPFRN
                     NGRPRAPDLHGGGENLRFPDYVMLSFAPQIFLTAMVYDGVFERFPKLRGGVIESGAGW
                     VPEFLRELDISSRSFGRTDPYLQAMPMKPSEYIRRAVKFTPFPNEDTGRMIADAGADL
                     FLFSTDYPHPEGTNDPKGRFERMMQNIDEDAKDRFYRRNFLEMMGLVTPA"
     gene            complement(4390..6120)
                     /locus_tag="VGP50_12525"
     CDS             complement(4390..6120)
                     /locus_tag="VGP50_12525"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEV8028250.1"
                     /translation="MPDDAPKKPLRSSHWFGKADRDGFVHRSWMKNQGLPHHLFDGRP
                     VIGICNTWSELTPCNAHFRKIADHVKRGVYEAGGFPLEFPVMSLGETLMRPTTMLFRN
                     LVSMDVEETIRANPLDGVVLLTGCDKTTPALMMGAASCDLPAIVVSGGPMLNGKFRGT
                     DIGSGTNVWSMSEDVRAGKMSLADFMDAESCMSRSAGHCMTMGTASTMASMVEALGMG
                     LPTNAAIPAVDSRRYVLAHMAGRRAVDMVREDLRMSKVLTRPAFENAIRVNAAIGGST
                     NAVVHLLALAGRVGVDLALADFDRLGHDVPLLVNLMPSGRFLMEDFYYAGGLPAVIRE
                     LGDLIHKDALTVNGSSIGENNAEAPCWNREVIHTLAAPLKEKAGIAVLSGNLCPDGAV
                     IKPSAATPALMRHKGGAVVFETIEHFKERVDAPDLAIDETSVMVLKGAGPRGYPGFPE
                     VGNLPLPKKLLKRGITDVVRISDARMSGTAYGTVVLHVSPEAAAGGPLALVQDGDTIE
                     LDVEKRRLHLDVAEAELAKRRQAWKPAPAAFERGYARLYVEHVMQAHQGADLDFLVGK
                     SGAKIARESH"
     gene            6367..7104
                     /locus_tag="VGP50_12530"
     CDS             6367..7104
                     /locus_tag="VGP50_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8028251.1"
                     /translation="MNQIDLANRVAVVTGGARGIGFAAAERCARSGAKVALWDRDGAE
                     AAARRIAGAIGCAVDVTDEAAVAAALALTEKRLGPIDILVASAGITGPNASVAEYPAA
                     AWRQVIEVDLTGVFLCCRAVVPGMTARNYGRIVTIASIAGKEGNPNAAAYSAAKAGVI
                     ALTKSLGKELAATGLRVNCVTPAAVKTELFAQMTESHIQYMLSKIPLGRFGTVEEIAA
                     MIAWLASEECSFTTGGVFDLSGGRETY"
     gene            7128..7655
                     /locus_tag="VGP50_12535"
     CDS             7128..7655
                     /locus_tag="VGP50_12535"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEV8028252.1"
                     /translation="MLAEALRLAGRGFWDELGALLARRPDLATLEDDDGRTLLMRCAG
                     LGGSAMTLRKLVALGADPNHRAKDNSTALAAAITGDAPHGPATLPELATLLDLGADPE
                     AVADGGMPALHWAIAQHRPEHVELLLDYGARLDTLTSGSPPESAQDIARRVQSAASLQ
                     LLDEYRRRRATAGET"
     gene            7659..>7859
                     /locus_tag="VGP50_12540"
     CDS             7659..>7859
                     /locus_tag="VGP50_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028253.1"
                     /translation="MPVRPSLALRAAAGDPAAQFKLARRLWQGRRRGAKRERDQAMTL
                     YRSAARAGDREAQAALAQIHLQS"
BASE COUNT         1323 a         2591 c         2638 g         1307 t
ORIGIN      
        1 tggcccacgc catgatccgc gagatcgacg gacgcattcg cttctggtcg agcagtctcg
       61 agcgccttta cggctggaag cgcgaagacg ccatcgggca aatatcacat gacctgctga
      121 gtacggaatt cccgaagccg ccggcggaga tcgaagcgga gctgctggcg acccgccgct
      181 gggaaggcga gctcaggcac cgcagccgtg acggccgaac cgtcatcgtg gcgagccatt
      241 gggcgctgga ccggaaggac ctcgggcaac ccgcctgggt tatcgaggtc aacaacgaca
      301 tcacggcctt gaaggcggcg gaggaggagc gcaatcgctt caatgccgcg ctcggcgagg
      361 cgcatcagcg gctcgggctt gcgatcgatg ccgggcagat cggtacctgg gagttggagc
      421 tccggaccgg ccggatgcgc cactccgccc gccgcgccgc gctgatgggg ctgcctgccc
      481 tcgccgtcga ggagcattac gaggaattcc tcgcgcgcgt tcaccccgac gaccgccaaa
      541 tggttgcgcg gatgctgtcg gcgtcggtcg aggacggccg cgaattccgc ggcacgttcc
      601 gtttcgtgtg gcccgacggc tcgatccatt gggtggaaag ccaggcggcg ccgccccgcg
      661 accggtcggc ggacccggtt cgcatcatgg gcgcgtccat cgacatcacg gagcgcatgc
      721 tgcgcgaagc cgagctgcgc cgcgccaagg aggcggcgga gcgggcaacg caatcgaaga
      781 gtcgcatgct tgccgccgtg ggacatgacc tgaagcagcc gctgcatatc atcgggatgg
      841 cgctgagcat gttgcaacga cgcgtcgccg acctcgatcc gaaacggctc ctgatccgcg
      901 ccgatcgggc agtggaccag cttgcggccg cgctcgacac gctgctcgag gtcgcgcagc
      961 tcgaggccgg tgtcgtcgcc ccgcggatcg aagcctttcc ggtcgagcgg atcctgaacg
     1021 acatcgaaga ccagttccgc cttcttgccg ctcacaaggg gctgtccttc acggtcgagc
     1081 attcgagcgc actcgtccgc agcgacccca aaatgctcgc gacgatcctg cacaatctcg
     1141 tgggcaacgc catcaaatac accgaggagg gccggatcga cgtcgcctgc cggcaacacg
     1201 acgccgtcct ctcgatcgag gtccgtgaca ccggcatcgg catcccgccg gacaagctcg
     1261 agctcgtatt cgaggagttt caacgcctcg agaccagcag cagcaaaggc ggcctcggcc
     1321 tcgggctggc gatcgtccgg cacaccgcca atgtcctggg ccaccccctc cgcgttcagt
     1381 ccaacgtcgg ccagggctcc gtcttcagcg tcgacattcc cgcgggctcc taggcgaaca
     1441 cgcttcgcgt gctcgcctag gagcccgcag cggcgacagc gattgccctt gcaatcgcga
     1501 gagccccagg cgaacacgca aagcgtgttc gtccgcggcg gcgacagcga ttgctcttgc
     1561 aatcgcgaga gccgcaccgc gcagattagc tgatcgcgat gcccccacac cctgccctct
     1621 ccccgatcgg gggaaagggc aagatcgagt cagcgcgatc ccgattccgc tcagagcgga
     1681 atggcgaagg cgaaccacac cgtgctggtc gacgccgtgt tcttggcgaa gaggtcgcgc
     1741 gtgtaataaa ggttcatgac gatcgggccg aactcgtaga agacgctcgg gccaagagag
     1801 agactctgga agcgattgcc cgcgctgttg cccagattga ggaaattcgc cggcacccgc
     1861 ccgggcgctg cccccacggc ggcgagcgcc gcgggattgc cgctcagcgt gtcatccgtc
     1921 gtctgagtgg cgaaactccc gacgatgccg acactccatt tgtggtagac gcccggaagg
     1981 gtctggagcg cgccaaaatc gagggtcagg aagtcgccgc tctggtagct gatttgtgag
     2041 ccgccgacat tgaggaagtt gttgtccttg gtctggatgt catattcggc attgacggaa
     2101 agctgcgtcc catggccgtt gaaccacgcg agataggccg atggcgagaa gatccagtaa
     2161 tcggcagcgt tgtggggggt gacgctggtg ccgcccttgg cgtgggcctc gttgtcccca
     2221 tcgtcgatgg cgatcggcgt gctgatggcc gcgaagaagc cggccggcaa gtgccacgac
     2281 aagaccagcg gcgagaaggc cgtgttgtcg aggcccgtct tgttggtcgt cacgctcgtc
     2341 aggctgtggg ttatcggcga taccgatgtg gtcgtcgccg agttggtgac gatcggctgc
     2401 agcagggtgg cgccatactg ggcaccgagg atgtgaaacg gcgacgacca ggtgagcacc
     2461 gggatttcga cataggcggt gacgttcacg gcggtgtgcg ccggcgcctt gttgacgttg
     2521 ccattaccgt catcgaggct gcccgacaca tagttgaagt tgttgacgaa gtagaatccg
     2581 ggcggcggca atgcggcgag cggcgcaccg gcggcggctc cgggctgatg aggatcggtg
     2641 tcgcccggct cgaccgcatg gcactgggcc gcccatgcaa gcgaagcgag cccggccaga
     2701 gcgccgacgg cccgcgaaaa tgcgagattc ttcattacgt tgtcccctcc ccgaaatgaa
     2761 tgacggcccc gcccggtcgt aagctccctc caccggacga agacggatga acgtcaccga
     2821 ttgttgtctt ttagtgacta ttcatcgtct ctattaggct tcggctgcgg cgccgagaca
     2881 agatcgggcg gaatgcccgc aaccgcctgg tatcgcggcc ttactttgtc atggcgcggc
     2941 tatcgccttg actcgattga tgaatatgaa tgaaaccccg gcgccgccgc aatgttgcaa
     3001 tccggcaaca tgacgcgggt ttctggcgga cgaggacgtg gaccctgctt ccgccgacgc
     3061 cctctcggcg tttccttggg ctaggctggg gtgacgagac ccatcatctc caggaaattt
     3121 cgccgataga agcgatcctt cgcgtcctcg tcgatgttct gcatcatgcg ctcgaagcgg
     3181 cccttggggt cgttcgtgcc ttcgggatgc ggataatcgg tcgagaacag gaaaagatcg
     3241 gcgccggcat cggcgatcat tcggcccgta tcctcgttcg ggaagggcgt gaatttgacg
     3301 gcgcggcgga tgtattccga cggcttcatc ggcatggcct gcagataggg atcggtccgg
     3361 ccgaagctcc tggagctgat gtccagctcg cgcaggaatt cggggaccca gccggcgccc
     3421 gactcgatga cgccgccccg caatttgggg aaccgctcga agacgccgtc atagaccatg
     3481 gctgtcagga agatctgggg cgcgaaagag agcatgacat agtccgggaa ccgcaggttc
     3541 tcgccgccgc catgcaggtc gggcgcgcgg ggccggccgt tgttgcggaa aggggccggc
     3601 tggttcaggc tgccgggccc gatatgcagg atgaagggcg tgtctgtttc gctgagacgc
     3661 gcccatagcg ggtcgaggtc ggggtgcccc ggggagcgct cgcccgcggg cccggcggaa
     3721 accatgatcg ctcggctgcc gatcctcaag gccgcgtcga cttcttcgag agcgcgcttg
     3781 ggattgtcga gcggcgcgta ggcgacgccg aagagacgct tgtcattgcg gcagaaagcg
     3841 gccatcgctt cattgcgcgc gcgcacgccg gcatacatca acccctcgtc cttcgcctcg
     3901 agcgccggcg cgagcgagaa ggtaccgaac accaattgcg ccgagaagcc gaacacgtcg
     3961 aggacgcgcg agcgctcggc gggatcgaag gcgcccgcgg caagccagcc cttgggaccg
     4021 ccgatcgggt tttcgaacgc tgctctctcg gcgtctttgt cccctttgcg cgcctttgcc
     4081 tgttccagca gcttcgcgat ccgcccgggg tcgcggctat agagcggcct gagcttcttc
     4141 ttgaggtcgt catccatgaa gggctccagc cactcgcgcg tctccatgac atgactgtcg
     4201 gcatcgtaga cgatccgtcc cgcggtatag gtcatggcca gctccgtccc ttgcatgatc
     4261 ggtcatagaa tacgcgcgga ccccttaggg ctgcaagtaa agcggcgagc gcgaagcgct
     4321 cgtccgcggc ggcgtcagcg attgcgcagc aatcgcgaga gccgcaccga tcgggttagc
     4381 cgcgaccgcc taatgcgact cccgtgcgat cttcgcgccg ctcttgccga cgaggaagtc
     4441 gaggtcggcg ccttgatgcg cctgcatcac atgctcgaca tagagcctgg cgtagccgcg
     4501 ctcgaaggcg gcgggcgccg gcttccaggc ctgacggcgc ttcgccagct ccgcctccgc
     4561 cacatcgagg tgcagccggc gcttctcgac gtcgagctcg atcgtgtcgc cgtcctgcac
     4621 cagcgccagc ggcccgccgg ccgccgcttc cggtgacacg tggagcacga ccgtgccata
     4681 ggccgtgccg ctcatccgcg catcggagat gcgcacgacg tcggtgatgc cgcgcttcag
     4741 gagcttcttc ggcagcggca ggttgccgac ttcggggaat cccggataac cccggggccc
     4801 cgcccctttc agcaccatga cgctggtctc gtcgatggca aggtcgggcg cgtcgacgcg
     4861 ctccttgaaa tgctcgatgg tctcgaacac cacggcgccg cccttgtggc gcatgagcgc
     4921 gggcgtcgcg gccgacggct tgatgacggc gccgtcggga cagagattgc ccgacagcac
     4981 ggcgatgccg gccttctcct tgagcggcgc cgcgagcgtg tggatgacct cgcggttcca
     5041 gcagggggcc tcggcattgt tctcgccgat gctgctgccg ttcacggtga gcgcgtcctt
     5101 gtggatgaga tcgcccagct cgcggatcac cgcgggaagc ccgcccgcat aatagaaatc
     5161 ctccatgagg aagcggcccg acggcatcag gttgacgagc agcggcacgt catggccgag
     5221 ccggtcgaaa tccgcgagcg ccaggtccac gccgacgcgg ccggcaagcg ccaggagatg
     5281 caccacggca ttggtcgagc cgccgatcgc ggcattgacg cggatcgcat tctcgaaggc
     5341 cggccgcgtc agcactttcg acatgcggag atcctcgcgc accatgtcga cggcgcgccg
     5401 cccggccata tgcgccagca catagcggcg gctgtcgacc gcggggatcg cggcattggt
     5461 cggcagcccc atgccgagcg cctcgaccat gctggccatg gtcgaggccg tgcccatggt
     5521 catgcaatgc ccggccgagc gcgacatgca ggattccgcg tccatgaagt cggcgagcga
     5581 catcttgccg gcgcgcacat cctcgctcat cgaccacaca ttggtgccgg agccgatatc
     5641 ggtgccgcgg aatttgccgt tgagcatcgg cccgcccgag acgacgatcg ccggcaggtc
     5701 gcagctcgcc gcgcccatca tcagcgccgg cgtggtcttg tcgcagcccg tgagcagcac
     5761 gacgccgtcc agcggattgg cgcggatcgt ctcctcgaca tccatgctca cgaggttgcg
     5821 gaagagcatc gtcgtcggcc gcatcagcgt ctcgcccagg ctcatcaccg ggaattcgag
     5881 cgggaagccg ccggcctcgt agacgccgcg cttcacgtga tcggcgatct tgcggaaatg
     5941 ggcgttgcag ggcgtgagct cggaccaggt gttgcagatg ccgatgaccg ggcgcccgtc
     6001 gaagagatga tgcggcagcc cctggttctt catccaggag cgatggacga agccgtcgcg
     6061 gtcggccttg ccgaaccagt ggctgctgcg cagcggcttc ttgggcgcgt cgtcgggcat
     6121 cgcttcctcc tggtttcgcg cgggttgacg gcgaccgccc ccgccttgtc gaatgttgcc
     6181 gcatcttacc gatacgggcg cgcaagtcac agatgcgcgc atctctataa ctcctctccg
     6241 cccctcgggg cggagagggt cggggtgagg tggggcatct gggtcgcgac cgatggaccc
     6301 aagacccacc tcatcctaaa tccttctccg ccctgaaggg cggagaagga cttgagagga
     6361 gccgcgatga accagatcga tctcgcaaac cgcgtcgccg tcgtcaccgg cggtgcccgc
     6421 ggcatcggct tcgccgcagc cgagcgctgt gcccgctcgg gcgccaaggt cgcgctctgg
     6481 gaccgcgacg gcgccgaagc ggcagcgcgg cgcatcgccg gcgccatcgg ctgcgccgtc
     6541 gacgtgaccg acgaggccgc ggtcgccgcg gcgctggcgc tcaccgagaa gcggctcggg
     6601 cccatcgaca tcctcgtcgc cagcgccggc atcaccggcc ccaacgcgag cgtcgccgaa
     6661 tatcccgcgg cggcctggcg ccaggtgatc gaggtcgatc tgacgggcgt cttcctctgc
     6721 tgccgcgccg tggtgccggg catgacggcg cggaattacg ggcgcatcgt caccatcgcc
     6781 tcgatcgccg gcaaggaagg caatcccaac gccgcggcct attccgccgc caaggccggc
     6841 gtgatcgcgc tcaccaagtc gctgggcaag gagttggcgg cgaccggcct gcgcgtcaat
     6901 tgcgtgaccc ccgccgccgt gaagaccgag ctcttcgcgc agatgacgga gagccacatc
     6961 caatacatgc tgtcgaagat cccgctcggc cgcttcggca cggtcgagga gattgccgcg
     7021 atgatcgcct ggctcgcctc ggaggaatgc tccttcacga cgggcggcgt cttcgatctc
     7081 tccggcgggc gcgagacgta ttgaaggagc gttgagactt ggacacgatg cttgccgaag
     7141 cgctcaggct tgccggaaga ggcttctggg acgagctcgg cgcgctgctc gcgcggcggc
     7201 ccgatctcgc gactctggaa gacgacgacg ggcgaacgct gctgatgcgc tgcgccggtt
     7261 tgggcggctc ggccatgacc ctgcgcaagc ttgtcgcgct cggcgccgat cccaatcatc
     7321 gcgccaaaga caattcgacg gcgcttgcgg cagcgatcac cggcgacgcg cctcatggtc
     7381 cggcgacctt gccggagctc gcgacattgc tcgacctcgg cgccgatccg gaggccgttg
     7441 ccgacggcgg aatgccggcg ctccattggg cgatcgcgca gcaccgtccg gagcatgtgg
     7501 agctcctcct cgactatggc gcccgcttgg acacgctgac gagcggcagc cctccggaat
     7561 cggcgcagga catcgcgcgc cgcgtccagt cggcggcatc gctgcagctc ctcgatgaat
     7621 atcgccggcg ccgcgcgacg gcgggcgaga cttaggagat gcccgtgcga ccctcgctgg
     7681 cgttgcgcgc cgccgcgggt gatcccgcgg cgcaattcaa gctcgcccgc cgcctctggc
     7741 aagggcgacg gcgtggggcc aagcgcgagc gcgaccaggc gatgacgttg tatcgcagcg
     7801 ccgcccgcgc gggcgatcgc gaagctcagg cggcgctcgc acaaatccat cttcagagc
//
LOCUS       DASYNS010000404         3328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4939284, whole genome shotgun sequence.
ACCESSION   DASYNS010000404 DASYNS010000000
VERSION     DASYNS010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4939284"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VGP50_12545"
     CDS             complement(<1..521)
                     /locus_tag="VGP50_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8028254.1"
                     /translation="MVERMQEGALTLTSDGTVLYANQRLGSFLGLALPNIVSQKFEQF
                     VAADDRDSFVELLTRRGPAGGKGEFALRAADGTAVPVYLSVVDLPDEGQGIISGIVTD
                     LRWQKQRMQELAEANAKLVASIAERSRAEAMLRQAQKMEAVGQLTAGIAHDFNNLLMV
                     IGGNLELFIGRTG"
     gene            complement(723..1046)
                     /locus_tag="VGP50_12550"
     CDS             complement(723..1046)
                     /locus_tag="VGP50_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEV8028255.1"
                     /translation="MTGADSLTLAGAERYVLRLYVTGSTKHSTRAIETTRQICDTHLK
                     GRHDLEVIDLYLNPEAAAREQIIAAPTLVKLLPAPLRRIIGDLSDQPRVLAGLGLSIP
                     KAVAS"
     gene            complement(1043..1330)
                     /locus_tag="VGP50_12555"
     CDS             complement(1043..1330)
                     /locus_tag="VGP50_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEV8028256.1"
                     /translation="MDERYNLRLYVAGQTPRSLAAIANLRLLCDTYLAGRHSVEIIDL
                     VDKPHLAILDQILALPTLVRHLPKPLKRIIGDLSRTEHVLLGLDIESRRAL"
     gene            complement(1330..3099)
                     /gene="kaiC"
                     /locus_tag="VGP50_12560"
     CDS             complement(1330..3099)
                     /gene="kaiC"
                     /locus_tag="VGP50_12560"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEV8028257.1"
                     /translation="MKPMETAPAAPHLAKAPTGIPGLDEVLLGGVPAGRPTLICGSAG
                     CGKTLLGLTFLVKGATQFGEPGVFVSFEETSRDLAENVASLGFDVPALIAAKQLAIDH
                     IRVERSEIEETGEYDLEGLFVRLGHAIDQIGAKRVVFDTLEALFSNLSNEAILRAELR
                     RLFAWLRDRGLTAIITAERGHGTLTRHGLEEYISDCVILLDNRVQDQVTTRRLRIVKY
                     RGSAHGTNECPFLLDDQGITVVPLSSAGLGHRVSGEVVSTGVPGLEAMLGPGGVHRGS
                     SVLISGVAGSGKTILGAAFAAAACARGERCMFFSFEESAEQLARNVGSAGIDLQQHVE
                     IGRLRFETKRPTSFGFEMHLAHMQRELDRFGPEAVIVDPVTSFRGPDSEVHALLLRML
                     DILKTRGITTIFTSLTSSEERMAQGDFGLTSLMDTWISLVDIESNGERNRGLYVLKAR
                     GMSHSNQIREYLLTDAGVKLVEACLGAAGVLTGSARLAKEAQERMEVQQQGEELVRRR
                     REIASRRAATERQISEPHAAIAAQDVEVARLVAQEERRETVVERDRAAMGASRGVAYL
                     TGEGKSRPASPMLRETREPARTP"
BASE COUNT          557 a         1099 c         1103 g          569 t
ORIGIN      
        1 tcgcccgtgc gcccgatgaa caattccaga ttgccgccga tcaccatcag gaggttgttg
       61 aagtcgtgcg cgatgccggc ggtgagctga ccgacggcct ccattttctg cgcctggcgc
      121 agcatcgctt ctgcccggct ccgttcggcg atggacgcga ccagcttggc gttggcctcg
      181 gccagctcct gcatccgctg cttctgccag cgcagatcgg tgacgatgcc ggagatgatc
      241 ccttggccct cgtccggaag atcgaccacc gacaggtaca ccgggacggc cgtgccatcc
      301 gccgcgcgca gcgcgaattc gcccttgccg ccggccgggc cgcgccgcgt cagcagctcg
      361 acgaacgagt cccgatcatc ggcggcgacg aattgctcga atttctggct gacgatgttc
      421 ggcagagcaa gaccgaggaa cgatccgagg cgttgattgg cgtagagaac ggtcccgtcc
      481 gacgtcaatg tgagcgcccc ttcctgcatg cgctcgacca tgttccgata gggacggtcg
      541 gcattgagca gcgtataaga cttgcgagcc ggacgcgcct ttgacgacga ccgcgtccac
      601 ctcgccgtgc cggatcgccc ggagcgtctc ctccgcttcc gcgaaccgtc ctttcagctc
      661 gtcgagctcg ccttggagcg gatgaggggc ggcggccaga tccggcgatg caagcagcgt
      721 gctcatgacg ccacggcttt tggaatgctg aggccaaggc cggcgagaac ccgcggctgg
      781 tcggaaagat cgccgatgat gcggcgcagc ggagcgggca gcagcttcac caaagttggc
      841 gccgcaatga tctgctcccg ggcggccgct tccgggttga ggtagaggtc gatgacctcg
      901 aggtcatggc ggcctttgag gtgggtatcg cagatttgcc gcgtcgtctc gatcgcgcgc
      961 gtcgaatgct tcgtgctgcc ggtgacatag agacgcaaca cgtagcgctc ggcgccggcg
     1021 agagtcagcg agtccgcgcc ggtcatagcg ccctccggga ttcgatatcc aacccgagaa
     1081 gaacatgctc cgtccgcgac aggtcgccga tgatccgctt gagcggcttc ggcaagtggc
     1141 gcaccagggt cggcagcgcg aggatctggt ccaggatcgc cagatgcggc ttgtcgacga
     1201 ggtcgatgat ctcgacggaa tggcgtcccg cgaggtaggt gtcgcagagc aatctgagat
     1261 tggcgatggc ggccagcgat ctcggcgttt gccccgccac atagaggcgg agattatatc
     1321 gctcatccat tacggcgtcc tcgcaggctc gcgcgtctcg cgcaacatcg ggctggccgg
     1381 gcggctcttg ccctcgccgg tcaggtacgc gacgccgcgg ctggcgccca tggcggctcg
     1441 gtccctttcg accaccgtct cgcgccgctc ttcctgtgcg acgagccgcg caacctccac
     1501 gtcctgcgcg gcgatcgccg catggggctc ggagatttgc cgctccgtcg ccgcgcgccg
     1561 gctcgcgatc tcccgccggc ggcgcacgag ctcctcgccc tgctgctgga cctccatccg
     1621 ctcctgcgcc tctttcgcca ggcgcgcaga gccggtcagc accccggccg cgccgagaca
     1681 agcctcgacg agcttgacgc cggcgtcggt caacagatat tcgcgaatct gattcgaatg
     1741 gctcataccg cgcgccttga gcacataaag gccgcggttg cgttcgccgt tgctctcgat
     1801 gtcgaccaga gagatccagg tatccatgag cgaggtcagc ccgaaatcgc cctgcgccat
     1861 gcgctcctcg gagctggtga ggctggtgaa gatggtggtg atgccgcgcg tcttcaggat
     1921 gtccagcatg cggagcagaa gggcatgcac ttcgctgtcc ggtccgcgaa agctggttac
     1981 cggatcgacg atgacggcct ccggcccgaa gcggtcgagc tcgcgctgca tatgcgccag
     2041 atgcatctcg aagccgaaag acgtcggacg cttggtttcg aatctcagcc gaccgatctc
     2101 gacgtgctgc tgcaggtcga tcccggccga gccgacgttc cgcgcgagct gctcggcgga
     2161 ctcctcgaag ctgaagaaca tgcagcgctc gccgcgcgcg caggctgcag cggcgaaggc
     2221 cgcgccgagg atggtcttgc ctgagcccgc gacgcccgag atcagcacgc tcgacccgcg
     2281 atggacgccg ccggggccga gcatggcttc gagtccggga acgccggtcg ataccacctc
     2341 gcccgagacc cgatggccga ggcccgcgga cgatagcggc acgaccgtga tgccctggtc
     2401 atccaggaga aagggacact cgttcgtacc gtgcgccgag ccgcgatatt ttacgatgcg
     2461 cagccgccgc gtcgtgacct gatcctggac gcggttgtcc aggaggatga cgcagtccga
     2521 gatatattct tcgagcccgt gccgggtgag cgtgccatgg ccccgctcgg cggtgatgat
     2581 cgcggtgagg ccgcggtcgc gcagccaggc gaagaggcgg cgcagctcgg cgcgcaggat
     2641 cgcctcgttc gacaagttgg agaacagagc ctcgagcgtg tcgaagacga cacgcttggc
     2701 gccgatctgg tcgatggcgt ggccgagacg gacgaagagg ccctcgaggt cgtattcgcc
     2761 ggtctcctcg atctcgctgc gctcgacgcg gatatgatcg atcgcgagtt gtttcgcggc
     2821 gatcagcgcg ggcacgtcga aacccagaga ggcgacattc tctgccagat cgcgggacgt
     2881 ctcctcgaag ctgacgaaga cgccgggctc gccgaactgc gtcgcgccct tgaccaggaa
     2941 agtcaggccc agcagcgtct tgccgcagcc ggcggagccg caaatcagcg tcggccggcc
     3001 ggcggggacg ccaccgagca ggacttcgtc gagaccggga atccccgtcg gcgccttggc
     3061 caagtgcggc gccgccggcg cggtttccat cggcttcatc caatattccc aaacctcagg
     3121 gttgctcgag tcgagcgaat aacgagcgta acgcgacccg ttccagcgcc gctaaagcag
     3181 agttgcgcag atcgcgacac gtatgcacaa atcccctctc ccctcgggga gagggtcagg
     3241 gtgagggggc cgcgccatga tgttattcag cggtggccgc gtgtttctgc acgcgtcatc
     3301 tcactggcgt cgccccctca ccccggcc
//
LOCUS       DASYNS010000405         8063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2129865, whole genome shotgun sequence.
ACCESSION   DASYNS010000405 DASYNS010000000
VERSION     DASYNS010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8063
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129865"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VGP50_12565"
     CDS             complement(<1..231)
                     /locus_tag="VGP50_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA synthetase"
                     /protein_id="HEV8028258.1"
                     /translation="MLIGAMLRLLDGARHIAVGAHSPVPAAAAFLAQLLSPDGALVSL
                     QQSPEHNVFTDGGRELFDSAGQGRIDVFFLSGA"
     gene            complement(254..1069)
                     /locus_tag="VGP50_12570"
     CDS             complement(254..1069)
                     /locus_tag="VGP50_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV8028259.1"
                     /translation="MSRICELEALIGEIEDGVMLAVPADYAGVAMAATRALIRRGVER
                     LHLVTLPTSSLQADLLIGAGAVATIETSAVSFGELGAAPRFTAAVLGGALQLKDATCP
                     ALHAALRASEMGNPFAPLRGLIGSDVLRERPDWRIIDNPFGSNDPIVLLPAIRPDVAL
                     FHAPLADRRGNVWVGRRRDLFTLAHASAKSVATVERLHDGDLLADETLAAATIPSLYI
                     AAAAVAPKGAAPLGFGDLYPEDTAHLLDYLRRAATSEGFARYLDEHVFAHRAA"
     gene            1583..2944
                     /locus_tag="VGP50_12575"
     CDS             1583..2944
                     /locus_tag="VGP50_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028260.1"
                     /translation="MKVFPSRWYLHGFCSAVGAAALAACCGILSIGPARALPSFARQT
                     GLECAVCHVAFPELTPTGRAFKLGGYVRQGGESDLPPLAAVVQGQFTHTQADQPGGAA
                     PHFAANDNFGLEAVSAFYGGAIYSPIGLGAFAQVTYDGAAHNFGWDNTDVRLAHTGEL
                     DDTPIIFGLSLNNNPTVQDVWNTTPAWRFPFVTSNVAPAPAAATLIEGGFAQNVVGLS
                     AYTFWNNLVYGEVGFYRSLGFQTLRKLGADTGVDVIDSVAPYWRFAVEPSWGKHSLEV
                     GTFGLTAATFPGRATTNGTDRRTDFGFDTQYQFIGDRDQISLQASFIHEAGRFNASQA
                     LGAATNLDDHLDSINIKTSYFYDHTYGINLSYFDISGSTDPGLFPGSANGSPNSNGWI
                     IEANYVPFMHGGPSFWPWLNIRFAIQYVIYDKFDGGRSNFDGAGRNASDNNTLFLLAW
                     LAF"
     gene            2967..3380
                     /locus_tag="VGP50_12580"
     CDS             2967..3380
                     /locus_tag="VGP50_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEV8028261.1"
                     /translation="MKQQAMIVLLAAAAIVVGASAWADSSAGVQEVRFDPAQIKEGKT
                     FFNDVCSTCHTDKPNKNKIGPSLFGVIGRHAGSAPGFDYSEAMKVAPVTWTDETLDRY
                     LADPKSVVPGNKMPYQGVKNDERRKEIIAYLHTLQ"
     gene            complement(4119..5153)
                     /locus_tag="VGP50_12585"
     CDS             complement(4119..5153)
                     /locus_tag="VGP50_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV8028262.1"
                     /translation="MTEAAALRFPFAAPPPPGTAVEVAPGVRWLRMPLPFQLNHINLW
                     LVEDGPGWTIVDTGVATDTTRELWEQIFATALDGHPVTRLVCTHFHPDHMGLAGWLTE
                     RLGIELWTTPVEWRHARAAIADAPGTFGGELADFYRCVGLDVTEETVRTSRPTPYRDL
                     VTPLPAEFRPLSEGMALAIGGRSWQVIIGRGHAPEHACLYCAELDVLIAGDQVLPKIS
                     PNVSLWPKAADQDPLGSFVASLDKLEGAVRATALVLPSHNLPFYGLHTRIGELRALHA
                     RRVADIVTACAQPQTGAELMPVLFSRTLDKHQLGFAIGETMAHVARAVALGALLREER
                     ADGVWLFRRT"
     gene            complement(5150..6361)
                     /locus_tag="VGP50_12590"
     CDS             complement(5150..6361)
                     /locus_tag="VGP50_12590"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEV8028263.1"
                     /translation="MQDAYVYDGLRTPIGRHAGGLAPVRPDDLLSGVIKAVVARNPFK
                     PGDYEDAVVGCTNQAGEDARNVARHAALLAGLPTAIGGLTVNRLCGSGLAAIADAARA
                     ASCGQGALFIAGGVESMSRAPFVMAKAESAYSREMKLFDSTIGARFPNPRIAKSFGDD
                     TMPQTADNVAHDHAISREDSDRFALASQEKYARAKAAGFYDSELMPVSVPGAKKGSET
                     VIAQDEHPRPNTTMDRLVELKPLFANGVVTAGNASGINDGAAALIIGARATGERAGKA
                     PRARILSAAIAGVEPRVMGLGPVPASKKALERAGLSLKDMDVIEVNEAFASQVLGCLK
                     LLGIDFDDARVNPNGGAIAIGHPLGASGARLALTAIRQLERSGGRYALVTMCIGVGQG
                     IAAVFERVGTA"
     gene            6960..7424
                     /locus_tag="VGP50_12595"
     CDS             6960..7424
                     /locus_tag="VGP50_12595"
                     /inference="COORDINATES: protein motif:HMM:NF037873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028264.1"
                     /translation="MTDTTGAALYRSVRKQDFPNGPIVDDHAVVGVLYPSFEPTDIGK
                     GRTRPPDVAVITDKHGQWVIPDGGGTSLFDKKVIKVFKEKYWWFFTIPKGTDIPGSLR
                     VRYTGRHEAFEAEHYQIEPAAGRMTVAAYKGALDNLARNAVVKAYEKAHGGA"
     gene            7427..7657
                     /locus_tag="VGP50_12600"
     CDS             7427..7657
                     /locus_tag="VGP50_12600"
                     /inference="COORDINATES: protein motif:HMM:NF044532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028265.1"
                     /translation="MFEVSVQTLMMAVQAVDAEISRIKESVGGDLGELDPDMQEFLLS
                     YGKAAMELKARYIEACRSISNLPPYEELVAGE"
BASE COUNT         1434 a         2605 c         2674 g         1350 t
ORIGIN      
        1 cgcgcccgag aggaagaaga catcgatgcg cccctggccg gcgctgtcga agagctcgcg
       61 gccgccatcg gtgaagacgt tgtgctccgg actctgctgc agcgatacca gggcgccgtc
      121 gggcgagagc agctgggcca ggaaggccgc ggccgcaggg accggcgagt gggcgccgac
      181 ggcgatgtgg cgcgcgccgt cgaggagacg cagcatcgcg ccgatcagca tttcctcgga
      241 tcggatggcc gtctcaggcg gcgcggtggg caaagacatg ctcgtcgaga tagcgggcga
      301 atccttctga tgtcgccgcc cggcgaaggt agtcgagaag atgcgcggtg tcctccggat
      361 agagatcgcc gaagccgagc ggcgcggcgc ccttgggcgc cacggcggcg gcggcgatgt
      421 agagagaggg aatcgtcgcc gccgccagcg tctcgtcggc gagcaggtcg ccgtcatgga
      481 gccgctcgac ggtcgcgacg ctcttggccg acgcatgggc aagggtgaag agatcgcgcc
      541 gccgcccgac ccagacgttg ccgcggcgat cggctaaggg cgcatggaag agcgcgacat
      601 cgggacgaat cgcgggcagc agcacgatcg ggtcgttgct gccgaacgga ttgtcgatga
      661 tccgccaatc cggacgctcg cgcagcacgt ccgagccgat gaggccgcgt agcggcgcga
      721 aggggttgcc catctcgctg gcgcgcagcg cagcgtgaag cgcggggcac gtcgcgtcct
      781 tgagctggag ggcgccgccc agcaccgccg cggtgaagcg gggcgcggct ccgagctcgc
      841 cgaaactcac cgccgacgtc tcgatcgtcg cgacagcgcc ggcgccgatc agcagatcgg
      901 cctggagcga ggatgtcggc agcgtcacca gatgcagccg ctcgacgccg cgccggatca
      961 gcgcccgggt cgctgccatc gcgacgccgg cataatcggc cgggacggcg agcatgacgc
     1021 cgtcctcgat ttcgccgatg agcgcttcca gctcgcaaat cctgctcaac gctcgggtcc
     1081 tcgccagcaa aacgcacaaa gcatgtccgg aaagccgcat gctgatcaaa tcataggcgg
     1141 cggattgttg aaaatccaaa agataccgcg cgagggagcg cgatcgagcg caagcacttc
     1201 cctcggccat tgttctcgcg cttcgccctc actcgaccct cactcagttg ttacatgacg
     1261 tgagacctcc tttcggacat tcgctggcat agtcacgtcg ctgatcgtga cgccgatgcg
     1321 gcgcgaagtc tgtgtgttgg caggagtcaa atgatgagcc acgtcaagtg gacgaacgcg
     1381 gtgcggatcg gcgattaccg ccggcgcctg ttgtggaccg aaagctcgtg agagcgccat
     1441 agcggccgcg acgccgcgtc gcccgccgcg cagcgcgaag tttcgctgct gcgcatagca
     1501 aaaacgtccc ttgcgaaacg agccggtttt ccagtcggcg ctcgaagagg ggacttgcgt
     1561 cgaaagggag tgcaagaggt cgatgaaagt ctttccgtca cgctggtacc ttcatggttt
     1621 ctgttcggcc gtcggggcgg ccgctcttgc cgcgtgttgc ggcattctct cgatcggacc
     1681 ggcgcgtgcg cttccgagct tcgcgcggca gaccggcctc gaatgtgccg tctgccacgt
     1741 cgcctttccc gagctgacgc cgacggggcg ggccttcaag ctcggcggct acgtcagaca
     1801 gggcggcgag tcggacctgc cgcctttggc cgcggtggtg cagggccagt ttacgcacac
     1861 gcaggccgac cagcccggtg gggctgcgcc gcatttcgcg gccaacgaca atttcggcct
     1921 cgaggcggtc agcgcctttt acggcggtgc catctacagt ccgatcgggc tcggcgcctt
     1981 tgcgcaggtc acctatgacg gcgccgccca caatttcggc tgggacaaca ccgacgtccg
     2041 cctggctcat acgggcgagc tcgacgacac gcccatcatc ttcggtctct cgctcaacaa
     2101 caacccgacc gttcaggacg tctggaacac gacgccggcc tggcgcttcc ccttcgtcac
     2161 gtcgaacgtc gcgccggcgc cggcggctgc gacactgatc gagggcgggt tcgcgcagaa
     2221 cgtggtgggc ctcagcgcct acaccttctg gaacaacctc gtctatggcg aggtcggctt
     2281 ttatcgctcg ctcggctttc agaccctgag gaagctgggc gccgacacgg gggtcgacgt
     2341 catcgattcc gtcgcgccct attggcgctt cgccgtcgag ccgagctggg gcaagcattc
     2401 gctcgaggtc ggcaccttcg gcctcacggc agcgacgttc cccggacgcg cgacgaccaa
     2461 cggcaccgat cggcgcaccg acttcggctt cgacacccaa taccagttca tcggcgaccg
     2521 cgaccagatc tcgctgcagg cgagcttcat ccatgaggcc gggcgcttca atgccagcca
     2581 ggcgctcggc gcggccacca atctcgatga tcatctcgac agcatcaaca tcaagacgtc
     2641 gtacttctac gaccacacct acggcatcaa cctctcttat ttcgatatca gcggcagtac
     2701 cgatccgggg ctctttcccg gcagcgccaa tggcagcccc aacagcaacg gctggatcat
     2761 cgaggccaat tacgtgccct tcatgcatgg cggcccatcc ttctggccgt ggctgaacat
     2821 ccgcttcgcg atccaatacg tcatctacga caagttcgac ggcggccgat ccaatttcga
     2881 cggcgcgggg cgcaatgcat ccgacaacaa cacgcttttc ctgctggctt ggctggcgtt
     2941 ctgaaacgac acgatggaga agagacatga aacagcaagc gatgatcgtc ctgctcgcgg
     3001 ccgcggccat cgtcgtcggc gcgtctgctt gggccgactc ctccgccggc gttcaggagg
     3061 tgcgcttcga tcccgcccag atcaaagagg ggaagacctt cttcaacgac gtgtgcagca
     3121 cctgccacac cgacaagccc aataagaata agatcggccc gtcgcttttc ggcgtcattg
     3181 gccgccacgc gggatcggcg ccaggcttcg actattcgga ggcgatgaaa gtcgcgccgg
     3241 tgacctggac cgacgagacg ctcgaccgct atctcgccga tccgaagagc gtcgttcccg
     3301 gcaacaagat gccctatcag ggcgtcaaga acgacgagcg gcgcaaggag atcatcgcct
     3361 atctccacac gctccaatag ccgtcgccgc ggcggcgccg gcgccggcgc tcgcttcgga
     3421 tcgcgcgggc atggcgcggc gtccgtggtc ttggaaccga cctgggaccg gccttgtgcc
     3481 cctcgtttct cgatccgacg ccgtggctcg gccttgttgt agatcaatga ccggacgctg
     3541 cgtctcggct atcttgtatc gatgattggc gcggcgaacg ccacggcacg cgattgtgtg
     3601 cgggcggcaa gaatcgaggc gaattggtgg cggataacga cgactttgac cgaaggtcgt
     3661 gccgcggctt cacgcttaat tattcagccg atgatagacc gaaaggacct cggcccgtgg
     3721 agcagcgatg atgagtgatc ccaacagttc tgtaacgcgg caccgctcgc tcgttcggct
     3781 cgtcgactat tctctcagtg aggcaagcga gctcgggctg ggcgacgtcg ctcaattcct
     3841 ggcgctcgcc tcggcgagtc tcgggaagaa cccgaggccc cgcgcgacta agaagtcgcg
     3901 caagaagcca tccagcggga ccgtggtgag tctcgccgag ttccgtgggc gcgaagaccg
     3961 gatggtgcgg cagaagtagc cgcccggcag ctggctccgc tccgcgtcga aaagtagtat
     4021 cccgatcccc cgagatcggc cagcgcgagc tcttccgcta ttgtcgctac gtcctgaagc
     4081 atgcgacggt cgcgcgcgaa catcgccagc gaccaggtct aggtgcggcg gaagagccag
     4141 acgccgtccg cgcgctcctc gcgcaaaagc gcgcccaatg cgacggcgcg cgcgacatgc
     4201 gccatcgtct cgccgatggc gaatcccagc tggtgcttgt cgagcgtccg gctgaacagc
     4261 acgggcatca attccgcgcc ggtctgcggc tgcgcgcacg ccgtgacgat gtcggcgaca
     4321 cggcgcgcat gaagcgcgcg cagctcgccg atgcgcgtat ggaggccata gaacgggagg
     4381 ttgtgcgacg gcagcaccag ggcggtcgcc cgcaccgcgc cctcgagctt gtcgagcgag
     4441 gcgacgaagc tacccagcgg atcctggtcc gcggctttag gccagagact cacattgggc
     4501 gagatcttcg gcagcacctg gtcgccggcg atcagcacat cgagctcggc gcaatagagg
     4561 caggcatgtt cgggggcgtg gccgcggccg atgatgacct gccacgagcg gccgccgatc
     4621 gcgagcgcca tgccctcgct cagcgggcgg aactccgccg gaagcggcgt cacgagatcg
     4681 cgataaggag tgggccgcga ggtcctgacg gtctcttcgg tgacgtcgag gccgacacat
     4741 cggtagaagt cggcgagctc gccgccgaag gtgccgggcg catcggcgat ggccgcccgc
     4801 gcatggcgcc actcgacggg cgtcgtccag agctcgatgc cgagccgctc ggtgagccag
     4861 ccggcaaggc ccatatggtc gggatggaaa tgcgtgcaga cgagccgcgt gacgggatgg
     4921 ccgtcgagcg ccgtggcgaa gatctgctcc cagagctcgc gcgtcgtgtc ggtggcgacg
     4981 ccggtgtcga caatggtcca gcccggcccg tcctcgacca gccagagatt gatatggttg
     5041 agctggaacg gcagcggcat gcgcagccag cgcacgcccg gcgcgacctc gacggctgtg
     5101 ccgggaggcg gcggcgccgc gaaaggaaaa cgcagcgctg ccgcttccgt catgcggtac
     5161 cgacccgctc gaacaccgcg gcgataccct ggccgacgcc gatgcacatg gtgacgagcg
     5221 catagcgccc gccgctgcgc tcgagctggc ggatggcggt gagcgccagc ctggcgcccg
     5281 aggcgcccag cggatggccg atggcgatgg cgccgccatt gggattgacg cgcgcatcgt
     5341 cgaagtcgat gcccaggagc ttgagacagc cgagcacctg gctcgcgaag gcctcgttga
     5401 cctcgatgac gtccatgtcc ttgaggctca agccggcgcg ctccagcgcc ttcttcgacg
     5461 ccggcaccgg gcccaggccc atgacgcgcg gctcgacccc ggcgatggcg gccgagagga
     5521 tgcgcgcgcg cggcgccttg ccggcgcgct cgccggtggc gcgcgcgccg atgatgagcg
     5581 cggccgcgcc gtcattgatg ccggacgcgt tgcccgccgt gacgacgccg ttggcgaaga
     5641 gcggcttgag ctcgacgagg cggtccatcg tcgtgttggg gcgcggatgc tcgtcctgcg
     5701 cgatcacggt ttcgctgcct ttcttggcgc ccggcacgct caccggcatg agctcgctgt
     5761 cgtagaagcc cgcggccttc gcgcgcgcgt atttctcctg cgaggccagc gcgaagcggt
     5821 cgctgtcctc gcggctgatg gcgtggtcat gcgcgacgtt gtcggcggtc tgcggcatgg
     5881 tgtcgtcgcc gaagctcttg gcgatgcgcg gattggggaa gcgcgcgccg atcgtcgaat
     5941 cgaagagctt catctcgcgg ctataggcgc tctcggcctt ggccatgacg aaaggggcgc
     6001 ggctcatgct ctcgacgccg ccggcgatga agagcgcgcc ctggccacag ctcgcggcgc
     6061 gcgccgcatc ggcgatggcg gccagcccgc tgccgcagag ccggttcacc gtgaggccgc
     6121 cgatcgccgt cggcaggccc gccagcagcg ccgcgtggcg cgccacgttg cgcgcgtcct
     6181 cgcccgcctg gttggtgcag ccgaccacgg cgtcctcgta gtcgccgggc ttgaacggat
     6241 tgcgcgccac caccgccttg atgacgccgc tcagaagatc gtcgggccgc accggcgcta
     6301 gaccgccggc atgacggccg atcggcgtgc gcagcccgtc atagacataa gcgtcctgca
     6361 tgctcatccc ctcgatccct cgggcccgcg ggagtaaacg ccggccgagc cttctatcca
     6421 ttgcgtgcga gacgggcagc ctagggaaac cgcgacggag cgacaagccg tggctggggc
     6481 atggctgcct tgagctagcg tgacggcgat cgcgcgccgg cgcgcgcata cctaggaaat
     6541 actaccgccc atgccatggc ctctagtctc gtcaacatcc gtgattgcct tatcaacgct
     6601 gtccgtggtc acgcggccac tagtctatca cgggtgtgag tgtagcttct gctcgtaccc
     6661 aaccagcgat tgtgctatgt gtgaacttta gccgtaggga agatcaggca tatgattgag
     6721 atcaccgatg tgactagaaa atatcatggc cgaattgtcc caatcatcca tcccggtatc
     6781 gaatccgtga cggactgggg tttcgtgcat gcggccgctg gcggcaaatt ggacgagttg
     6841 aatcgggcca cggcagcctc ggcgagctgc gcaggtgcgc agaggagatt ctcgcagcgg
     6901 agtggtagcc cagaccaccg ctgacaaaaa atgtctgaac gacacgaagg ggtttttcga
     6961 tgacggacac aaccggcgct gcgctgtatc gcagcgtccg caaacaagat ttccctaacg
     7021 gtccgatcgt tgatgaccat gcggtcgttg gcgtactcta cccgtcgttt gagccgaccg
     7081 atatcggtaa agggcggaca aggccaccag acgtcgccgt cattacggat aagcacggac
     7141 aatgggtcat cccggatggt ggcggcactt ccctgttcga caagaaggtc atcaaggttt
     7201 ttaaagagaa gtactggtgg ttcttcacga ttcccaaagg aacggatatc cctggttcgc
     7261 tgcgagtgag gtacaccggc cgtcacgagg cctttgaggc agagcattat cagatagagc
     7321 cggccgctgg aagaatgacg gtcgcggcgt acaaaggcgc cctcgacaac ctggcacgca
     7381 acgcggtcgt gaaggcatac gaaaaggctc acggaggagc ctgacaatgt tcgaagtgag
     7441 tgtacagacc ctcatgatgg cagtgcaggc ggtcgatgcg gaaatctccc gcatcaagga
     7501 atccgtcggc ggcgatctcg gcgagctaga tcctgatatg caggagtttc tactatcata
     7561 tggcaaggct gcgatggagc tgaaggcgcg ctacatcgag gcatgcaggt cgatatcgaa
     7621 tctgcctcct tatgaggagc ttgttgcggg cgagtaagca gcggaatccc tttccgcacg
     7681 gacctctgta tcggctaccg tagcggcgcg acaagccgca gacgacacgc cgccatgaac
     7741 ggccgggtta gcattcgcga acgcggaggt cgcagaggtt ccgcagagga cgcgaggaat
     7801 cactgttcct tcgcgtcctc cgcggaacct ccgcgtcccc cgcgttcccc tttcttttga
     7861 gaccgcggcg gcattgtgct ccgccgcgcg cttcccctag gattgcgccg gtgcggagga
     7921 ccatcacggt gaagcgcatc ttcgtcgatg gcgaggcagg gacgacgggg ctgcagatac
     7981 gtcagcgcct cacgcgccat ggcggcgtcg cgctcatctc gcttcccgag cagcagcgca
     8041 aggacacggc cgcgcgtgcc gag
//
LOCUS       DASYNS010000406         2619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3223818, whole genome shotgun sequence.
ACCESSION   DASYNS010000406 DASYNS010000000
VERSION     DASYNS010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3223818"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..359
                     /locus_tag="VGP50_12605"
     CDS             69..359
                     /locus_tag="VGP50_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028266.1"
                     /translation="MAGPKRLSPRIVQAIFQSTKPAAATAKRYKVSPNLVYLIRGRRI
                     HKATTIGLKSPQITRRESRKPLATPRFDVDQLVDAISKKVVRELVNRLRGKG"
     gene            complement(750..956)
                     /locus_tag="VGP50_12610"
     CDS             complement(750..956)
                     /locus_tag="VGP50_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028267.1"
                     /translation="MKIDDHKKAKPRHKGKDVKTNFDLKRDRERAAVASVGLRHRREA
                     VATAAGASAKPRSPAKQRRRMAAE"
     gene            1200..1742
                     /locus_tag="VGP50_12615"
     CDS             1200..1742
                     /locus_tag="VGP50_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028268.1"
                     /translation="MAYLPAAQRALDVLAPVVIYRQIGRRPSQVYVDDAMTEPTPGTK
                     AQHRLSDRVYWRTEAKPGDQIQDRRGGVILVTASGECYAITLAAPTPLSSQTAFTHAD
                     LSIRADQAEAERLIATGALVESSPRRPKRPLSRAADMLFPDDHPLVVEQLPKGVTLDA
                     DVGNFGRSPGNWSRRRRPQG"
     gene            complement(1951..>2619)
                     /locus_tag="VGP50_12620"
     CDS             complement(1951..>2619)
                     /locus_tag="VGP50_12620"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEV8028269.1"
                     /translation="IGRLESAHRGTIFLDEIGDLPLELQAHLLRFLQERTIDRVGGSK
                     PIEVDARVICATNLDLAKAVREGRFREDLYYRLNVLSLRLPALRERGDDIELLAKFFL
                     RKFAAEHGRAIAGFSDDAWQRIREHSWPGNVRELIGSIRRALVMADSSSIAAHDLGLD
                     DAAVGGLATAAAGRPGRARVDATQLEASLARNGGKVTPTARELALSRMTVYRLMRRYG
                     LTGH"
BASE COUNT          441 a          855 c          895 g          428 t
ORIGIN      
        1 aattgacggg tcacagggaa ggaatagcgt gctaaaaacg ctgctgccca aaatagggag
       61 aaccagatat ggcaggccca aagagactct cgccacgcat cgtccaagcc atctttcaat
      121 caaccaagcc ggctgccgcg acggcgaagc gctacaaggt ttccccaaac ctggtctatt
      181 tgattagagg acgtcgcatc cataaagcca ccacgatcgg gctgaagagc ccacagatca
      241 caagacgcga gagccggaag ccgctggcca ccccgcggtt cgatgtggac cagctcgtgg
      301 atgcgatttc aaagaaagtc gtcagggaac tggtcaaccg actgcgtggg aaggggtgac
      361 aatatccccc gacacaccaa tcgcgcagcg cggcgccagg atgtagacga gcattcctcg
      421 ccgcgccgtt tccgatagca tcgcgcgacg gctcgggatc agccgccgcc cgagggtccg
      481 gatcgggcgc gcgaggtcgg gtatgatggc gacccatccg cgcgcggccg atgcgagggg
      541 cgggtcaagc tgttcgcgcg gacgagtctc gaccgggcga aggcggggaa gcttgcgcaa
      601 agcacgggag cgaaaatcgc tcggcgtcgt cgagataccg cagggccgtc gcgacgtcgc
      661 cagcgatgcc gtcacgatct ggggcaagac cgagactgca gcgccgcatc gcgtcgggcc
      721 gtctcggcgg gacgaccgtt gctgcggcgc tattccgccg ccatgcgccg gcgctgcttg
      781 gccgggctgc gcggctttgc cgaagcaccg gcagcggtcg cgaccgcttc gcggcgatgg
      841 cgcaggccga cgctcgcgac ggcggcgcgc tcgcggtcgc gcttcaggtc gaagttggtc
      901 ttcacgtctt tgcccttgtg ccggggcttc gccttcttat ggtcatcgat cttcatcgcc
      961 ggtcctcggt tgatcgcgaa atgcgccagg gggcgatgct aaggggcgag ttactaacgg
     1021 agggtttccc ggcgctcgtc ccgcccggaa atctcttggt aattccgctg tggcaataat
     1081 cacccccgcg gccgctcgcg gagcccgaag gctctcgcga tttcagcggc ttaccgcgcg
     1141 tggtcgcatt tttgcccggc acaccccatc taatggggcg agaggcgagg gaaatcggca
     1201 tggcttatct acccgcggca cagcgtgcgc tcgacgttct ggcgcccgtc gtcatctatc
     1261 gccagatcgg ccgccggccg agccaggtct atgtcgacga cgcgatgacc gagccgacac
     1321 ccggcaccaa ggcgcagcac cgcttgagcg accgcgtcta ctggcgcacc gaggcgaaac
     1381 ccggcgacca gatccaggac cggcgcggcg gcgtcatcct ggtgaccgct tccggcgagt
     1441 gttacgccat cacgctcgcc gcgccgacgc cgctctcgtc gcaaaccgcc ttcacccacg
     1501 ccgatctgtc gatccgcgcg gatcaggcgg aggccgagcg tctgatcgcc acgggtgcat
     1561 tggtggagtc gtcgccgcgg cgcccgaagc gcccattgtc gcgcgccgcc gatatgctct
     1621 tccccgacga ccatccgctc gtcgtcgagc agcttcccaa gggcgtgacg ctggacgccg
     1681 acgtcggcaa cttcgggcgc tcgcccggca attggagccg ccgtcgccgt ccgcagggat
     1741 aagcgagaga agattcagca gttacatccc aagaacggga aggcgcgcat cgagggcgcc
     1801 gtctttttct cacggtctcg ccgtctcacc gcgaggcttc tgcgtcgccg cgacgtggcg
     1861 atggtcgaca tgtcagcgcg gttgcgccgt tgatgttttg attcgggctg atggtggcaa
     1921 cgccggtggc ggctgccatg gtccgctcgc tcaatgtccc gtcaggccgt agcgccgcat
     1981 caggcgatag acggtcatgc gcgaaagcgc gagctcgcgc gctgtcggcg tgaccttgcc
     2041 gccgttcctg gcgaggctcg cctcgagctg cgtcgcgtcg acgcgtgcgc gacccggcct
     2101 cccggcggcc gccgtggcga ggccgccgac ggcggcgtcg tcgagcccga gatcgtgggc
     2161 cgcgatcgag ctcgaatcgg ccatcaccag cgcgcggcgg atggagccga tgagctcgcg
     2221 gacattgccg ggccaggaat gttcgcggat tcgctgccag gcgtcgtcgc tgaagcccgc
     2281 gatggcgcgg ccatgctcgg cggcgaattt gcgcaggaag aatttggcga gcagctcgat
     2341 gtcgtcgccg cgctcgcgca gcgcggggag acgcaggctc agcacattga ggcggtagta
     2401 gagatcctcg cggaagcggc cttcgcgcac cgccttggcg aggtcgagat tggtggcgca
     2461 gatgacgcgg gcatcgacct cgatgggctt gctgccgccg acgcggtcga tggtgcgctc
     2521 ctggaggaag cgcagcagat gcgcctgcag ctcgagcggc aggtcgccga tctcgtcgag
     2581 gaagatggtg ccgcgatgcg ccgattcgag ccggccgat
//
LOCUS       DASYNS010000407         4715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5405383, whole genome shotgun sequence.
ACCESSION   DASYNS010000407 DASYNS010000000
VERSION     DASYNS010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5405383"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VGP50_12625"
     CDS             complement(<1..508)
                     /locus_tag="VGP50_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028270.1"
                     /translation="MRRFAIDPLRVGAVLLAVFGGIGTSAAQVPSDNPEQPPTQLQTP
                     PPPPEGMPPAAPPAGQPGYAPAPGDATPMQPPGAAPPSYAPGPGAAAPPGYPPGPGAG
                     VPVAPAPPAEMIPPPPDRHWVWQPGGWAWNGYQYVWEPGRYVRPRHHGVWIAGRWVPG
                     PAGWVWVPG"
     gene            complement(622..1914)
                     /locus_tag="VGP50_12630"
     CDS             complement(622..1914)
                     /locus_tag="VGP50_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV8028271.1"
                     /translation="MAESLFEDQAPRPLADKLRPKLLAEVVGQDHLLAPRGAIGRMVA
                     AHRLASMILWGPPGCGKTTIARLLAEATAMHFEAISAVFSGVADLRKVFDAAKKRRGG
                     GQGTLLFVDEIHRFNRAQQDGFLPYVEDGTVVLVGATTENPSFELNGALLSRCQVFVL
                     HRLDDTALETLLARAESALGRTLPLDDGARQALKAMADGDGRFLLNLAEELAQLAQGT
                     LLDSAGLAAAVQKRVPLYDKAQEGHYNLISALHKSVRGSDVDAALYWLARMLAGGEHP
                     EYIARRVLRMAVEDIGLADPQGLVQALAAWQAYERLGSPEGELAIAQAVIYLATAPKS
                     TAAYAAFGEARRAAKESGSLMPPMHSVNAPTRLMKELGYGKGYVYDPDTPEGFAGQNY
                     FPDELPRQTFYAPVDRGFEREIKKRLDYWGKLRQRKPE"
     gene            complement(1916..3325)
                     /locus_tag="VGP50_12635"
     CDS             complement(1916..3325)
                     /locus_tag="VGP50_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEV8028272.1"
                     /translation="MSLRRLFLSLALVLLALSAAAETRVVPENREVLRFSYAPIVKKV
                     APAVVNVFSRRVLRSVGGPALLFNDPFFRRFFGNQFPTGVPRERVENSLGSGVIVEAG
                     GLIVTNHHVIKDAEEVRVVLADRREFEAKILLSDERADLAVLKIDPQGDPLPVLPIGD
                     SDELEVGDLVLAIGNPFGVGQTVTSGIVSGLARSIGAADFRSFIQTDAAINPGNSGGA
                     LVDLDGRLIGINTAIFSQSGGSIGIGFAIPTNLVRTLLQAAANGGRVVRPWLGAAGQA
                     VTSDIAQSLGLSHPQGVLIKDVMAGSPAAQAGLRKGDVILAVDGHEVDDPEALRFRVA
                     TLALGARAQLSLWRGGVHRNAGVTIAAPPEEPPRQITPLVGRQPLAGATIANLNPAFD
                     DELGFDTTQHGVVVTALADGSVAERLGIEPGDIVVSLNKQPVETVAQLQQLLGTSRAP
                     WVVTVRRGDKLLSTPPLRG"
     gene            3579..4610
                     /locus_tag="VGP50_12640"
     CDS             3579..4610
                     /locus_tag="VGP50_12640"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV8028273.1"
                     /translation="MNQDHHLHAHPTVAIVGASGAVGTAFLHCLERRRFPARELRLFA
                     SARSAGKTLRFNGEPLAIHALTDASFGGVDIAFFSASSAVSKRFAPLAVRAGAVVLDN
                     SSAFRMEAQVPLVVPEVNAELIRDHQGIIANPNCVAAIACAALWPIHRRNRIRRLMLA
                     TYQAASGAGAAAMEELRQSTRAFLDGHPFEHRVLRHPYAFNLFSHDSAVDPATGYNGE
                     ETKVMAETRKIFADPELRVLATCIRVPVLRSHAMAVALECERPIAPEEARAMLADASG
                     VRVVDDVERNYFPMSNDASGADDVLVGRIRRDVSDPTGRSLALFVAGDQLLKGAALNA
                     VQIAERLVA"
BASE COUNT          750 a         1589 c         1646 g          730 t
ORIGIN      
        1 ggccgggaac ccagacccag ccggcgggtc ctggcaccca acgcccggcg atccatacgc
       61 catggtggcg cggccgcacg taacgcccgg gctcccacac atattggtag ccgttccatg
      121 cccagccgcc cggctgccag acccagtgcc ggtcgggcgg tggcgggatc atttcggcgg
      181 gaggcgcggg cgcgacgggt accccggcac cgggacccgg cgggtagccc ggcggtgcgg
      241 cggcaccagg acccggcgcg tagctcggcg gcgcggcgcc tggtggctgc atcggcgtcg
      301 catctcctgg tgcgggcgca tagcctggct gcccagcggg aggcgcggcc gggggcatgc
      361 cctccggcgg gggcggcggc gtctgaagct gcgtgggcgg ctgctctggg ttgtcggacg
      421 gcacttgggc ggcgctcgtg ccaatgccgc cgaagacggc caagagcacg gcgccaacgc
      481 gcaacgggtc gatcgcgaaa cggcgcatgg gggcgctcct tagaatgcga gaagatgcga
      541 cgaatttctc cgacggatta taggcgcgaa agcgggtcgg cgtgcccgga gcttagccgc
      601 ccaggcctcg gccggccacc ctcattcggg tttcctctgg cgtaatttcc cccaatagtc
      661 gagccgcttc ttgatctcgc gctcgaagcc gcggtcgacc ggtgcataga aggtctggcg
      721 cggcaattcg tcgggaaagt agttctgtcc tgcgaacccc tcgggcgtgt cggggtcgta
      781 gacatagccc ttgccgtagc ccagctcttt catgagccgc gtcggcgcgt tgacgctgtg
      841 catcggcggc atcagcgagc cgctctcttt cgcggcgcgt cgcgcttcgc cgaaggccgc
      901 gtaagccgcg gtcgatttgg gcgctgtggc gagatagatc accgcctggg cgatggcgag
      961 ctcgccctcg ggcgacccca ggcgctcata ggcctgccag gccgcgagcg cctggaccag
     1021 gccttgcgga tcggcaaggc cgatatcctc gaccgccatg cgcagcacgc ggcgcgcgat
     1081 gtattcggga tgctcgccgc ccgccagcat gcgcgcgagc caatagagcg ccgcgtcgac
     1141 atccgagccg cgcaccgatt tgtgcagcgc gctgatgaga ttgtaatgcc cctcctgcgc
     1201 cttgtcgtag agcggcacgc gcttctgcac ggcggcggca aggccggcgc tgtcgaggag
     1261 cgtgccctgg gcaagctgcg ccagctcctc ggcgaggttc aggagaaagc gcccgtcgcc
     1321 atcggccatc gccttcagcg cctggcgcgc gccgtcgtcg agcggcagcg tccgcccgag
     1381 cgcggactcg gcgcgggcga gcagggtctc gagcgcggtg tcgtcgaggc gatgaagcac
     1441 gaacacctgg cagcgcgaga gcaaggcgcc gttgagctcg aaggaagggt tctccgtcgt
     1501 cgcgccgacc agcaccaccg tgccgtcctc gacataaggc aggaatccat cctgctgcgc
     1561 gcggttgaag cggtggatct cgtcgacgaa gagcagcgtg ccttggccgc cgccgcgccg
     1621 ttttttcgcc gcgtcgaaca ctttgcgcaa atcggcgacg cccgagaaga ccgccgagat
     1681 cgcttcgaaa tgcatcgccg tcgcttcggc aaggagcctg gcaatcgtgg tcttgccgca
     1741 gccgggcggt ccccagagaa tcatcgaggc gaggcgatgc gccgcgacca tgcggccgat
     1801 cgcgccgcgt ggcgccagga gatggtcctg gcccacgacc tcggcgagaa gtttcggccg
     1861 gagcttgtcg gcgagcggcc gtggcgcctg gtcctcgaac aggctctccg ccatctcagc
     1921 ctctgagggg cggcgtgctg agcagcttgt cgccgcgccg caccgtgacg acccagggcg
     1981 cgcgcgacgt tccgagaagc tgctggagct gcgccaccgt ctcgacgggc tgcttgttga
     2041 ggctcaccac gatgtcgccg ggctcgatgc cgaggcgctc cgcgacgctg ccatcggcga
     2101 gcgccgtgac gacgacgccg tgctgtgtcg tgtcgaaccc cagctcgtcg tcgaaggcgg
     2161 gattgagatt ggcaatcgtc gcgccggcga gcggctgccg gccgacgagc ggcgtgatct
     2221 ggcgcggcgg ctcctccggc ggcgcggcaa tggtgacgcc ggcgttgcga tgcacgccgc
     2281 cgcgccacag gctgagctgc gcccgcgcac cgagcgccag cgtcgccacg cgaaagcgca
     2341 gcgcttcggg atcgtcgacc tcgtgtccgt cgaccgcgag gatgacgtcg cccttgcgca
     2401 gccccgcctg cgccgcggga ctgccggcca tcacgtcttt gatgagcacg ccctggggat
     2461 gcgaaagccc gaggctttgc gcgatgtccg aggtgacggc ctggcccgcc gcgccgagcc
     2521 agggacgcac gacgcggccg ccattcgccg ccgcctgtag cagcgtgcgc acgaggttcg
     2581 tcggaatcgc aaagccgatg ccgatcgagc cgcccgattg cgagaagatc gccgtgttga
     2641 tgccgatgag ccggccgtcg agatcgacca gggcgccgcc cgaattgccg ggattgatgg
     2701 cggcgtcggt ctggatgaag gaacggaaat ccgcggcgcc gatcgatcgg gcaaggcccg
     2761 agacaatgcc gctcgtcacg gtctggccga cgccgaaggg gttgccgatg gcaaggacga
     2821 gatcaccgac ctcgagctca tcgctgtcgc cgatcggcag caccggcagc ggatcgccct
     2881 gcggatcgat cttcaggacg gcgagatcgg cgcgctcatc cgagagcaga atctttgcct
     2941 cgaactcccg gcggtccgcc agcacgacgc gcacctcctc ggcgtccttg atgacgtgat
     3001 gattggtgac gataaggccc cccgcctcga cgatcacgcc cgagcccagc gaattctcga
     3061 cgcgctcgcg cggcacgccc gtcgggaact ggttgccgaa gaagcggcgg aagaaaggat
     3121 cgttgaagag cagcgccggc ccgccgacgc tgcgcagcac ccggcgcgag aaaacattca
     3181 ccaccgccgg tgccactttc ttgacgatgg gcgcgtagga gaagcgcagc acctcgcgat
     3241 tttccggcac cacgcgcgtc tcggcagccg ccgacagggc gagaaggacg agtgccagag
     3301 acaagaaaag gcggcgcagc gacatcgcgg taatctagga gagtgagggg tgaggggcca
     3361 gcgtcgcggc tttacaacgg ccgccagcca tgttacctcg tcccgtcatg gatcggcgga
     3421 acgagaagcg caagacgacc ggcaacacgt cccccgcggg gcggctgagg gtctgcttgc
     3481 gttaacgtaa acgccagaga gtttcccaag ggccccgccg gatcggacgg ggccctttcg
     3541 ttttagggcg ctcgtctccg gccagcatgg agaagagcat gaaccaagac catcatctgc
     3601 acgcccatcc gaccgtggcg attgtcggcg ccagcggcgc cgtcggcacc gcgtttctgc
     3661 actgtctcga gcggcgccgt ttcccagcgc gggagctgcg gcttttcgcc tccgcccgct
     3721 ccgccgggaa gactctgcgc ttcaacgggg aaccgctggc cattcacgcg ttgacggatg
     3781 cgagcttcgg tggagtcgac atcgccttct tttcggccag cagcgccgtc tccaagcgct
     3841 tcgcgccgct cgcggtgcga gcgggtgccg tggtgctcga caactcctcg gcattccgca
     3901 tggaggcgca ggtgccgctg gtcgtcccgg aggtcaacgc cgagctcatc cgcgatcatc
     3961 aaggcatcat cgccaatccc aactgcgtcg ccgccatcgc ctgcgcggcg ctctggccga
     4021 tccaccgccg caatcgcatt cgccgtctga tgctcgccac ctatcaggcg gcgtcgggcg
     4081 cgggcgccgc cgcgatggag gagctgcggc aatcgacgcg cgcgtttctc gacggtcatc
     4141 ccttcgagca cagggtgctg cgtcatcctt acgccttcaa tctcttcagc catgacagcg
     4201 ccgtcgaccc ggcgacgggc tacaacggcg aagagacgaa agtgatggcg gagacgcgca
     4261 agatcttcgc cgatccggag ctacgcgtgc tggccacgtg catccgcgtg ccggtgctcc
     4321 gcagccacgc catggcggtc gcgctggagt gcgagcggcc gatcgctccc gaggaggcgc
     4381 gcgcgatgct cgccgatgcg tctggcgtgc gtgtggtcga cgatgtcgag cgcaactact
     4441 tcccgatgtc gaatgatgca tcgggtgccg atgacgtgct ggtagggcgc atccgaaggg
     4501 atgtgagcga tccgaccggc cggtcgctcg cgctcttcgt cgccggcgat cagctcctca
     4561 aaggtgccgc cttgaacgcc gtgcagatcg ccgagcggct cgtggcgtaa tcctgccagg
     4621 cccccgcgtc ggtgagggag acgagggaac gccgaggacg cggagggttc gcagaggacg
     4681 cagagggatg attcttcctc agcgtcctct gcgaa
//
LOCUS       DASYNS010000408         4548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5250453, whole genome shotgun sequence.
ACCESSION   DASYNS010000408 DASYNS010000000
VERSION     DASYNS010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5250453"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VGP50_12645"
     CDS             complement(<1..781)
                     /locus_tag="VGP50_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8028274.1"
                     /translation="MSAPLPLLEVEGLKKHFPVHKGVLASVVGYVHAVDGISFDIAPG
                     ETLGLVGESGCGKSTAGKAILKLIDPTAGTVKLRGERIDHLDRRAMRPYRRELQVVFQ
                     DPYSSLNPRLSVGEIVGEPLANYGVFRGKALEQRVAEIFAKVGLREEAMRRYPHEFSG
                     GQRQRIGIARALVLNPSLIVLDEPVSALDVSVQAQVVNLLRDLQQELKLAYLFIAHDL
                     AVVEHISHRVAVMYLGKIVELAEKDALFRAPLHPYTEALLSA"
     gene            complement(778..1782)
                     /locus_tag="VGP50_12650"
     CDS             complement(778..1782)
                     /locus_tag="VGP50_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028275.1"
                     /translation="MSGAPEPLLAIADLRTHFFTRDGVVRAVDGVSYDVGAGETLAVV
                     GESGCGKSVTALSILRLVPSPPGRIVGGAIRFAGRDLLEASEAEMRSIRGNEIAMIFQ
                     EPMTSLNPVLRISHQIGETLMLHQGLDRAAAAARTVEMLRFVNISEPERRARQYPHEL
                     SGGMRQRVMIAMALACNPKLLIADEPTTALDVTIQAQILDLMRTMKARSGAAIILITH
                     DLGVVAEMASRVVVMYAGRKVEEAQVLDLFLKPRHPYTLGLLASVPRLGATIGRTDVP
                     RLPEIPGVVPSLREEIRGCAFAPRCAFAVERCRRAAPPLEEKNPGHYAACFESARFAA
                     "
     gene            complement(1779..2651)
                     /locus_tag="VGP50_12655"
     CDS             complement(1779..2651)
                     /locus_tag="VGP50_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8028276.1"
                     /translation="MALAPAEIAPIVQRHWLGETARRHPTVMIGVMLLLAMAAMALLA
                     PYLGTVDPLAVAPVKRLRPPSELYWFGTDSYGRDVYSRTLYGARVSLTVGLSVAMFGT
                     ALGLAIGLVTGFVRRLDFVVMRVMDALMSVPPVLLAIALMALTKASLGNVVLAITITE
                     VPRVARLVRSVVLSLREQPFVEAAVAAGTGFWRTLLRHILPNTIAPLLVQATYICAAA
                     MITEAVLSFIGAGTSPEIPSWGNMMAEGRSYFQIKPYLVLFPGAFLSLTVLAVNLVGD
                     GLRDALDPRLARRL"
     gene            complement(2653..3594)
                     /locus_tag="VGP50_12660"
     CDS             complement(2653..3594)
                     /locus_tag="VGP50_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8028277.1"
                     /translation="MLAYVVRRLVATVFVMTFVAFFVFSLLYLTPGDPAATIAGDLAT
                     TDDIARIHRQLGLDDPFLVRFGHWAWGVLQGDLGTSIFTNLPVTTLIGQRVGPTVSLT
                     VIALIITLSFAIPLGVIAASKVGSWIDRLVMVVAVLGFSVPSFVLAYIAILCFSVWLD
                     WLPVQGYASLSEGFAPFIEHLILPSIVLGLIYGALIARITRAAMLEVLSQDYIRTAQA
                     KGLTEGKVLVGHALRNAAIPIVTVIGSGIALLIGGVIVTETVFAIPGIGRLTVDAILR
                     RDYPIIQGVTLVLSAVYVVINLLVDVSYTFFDPRVRF"
     gene            complement(3756..>4548)
                     /locus_tag="VGP50_12665"
     CDS             complement(3756..>4548)
                     /locus_tag="VGP50_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8028278.1"
                     /translation="QLQRNKDITVANVDPLGSIGVMRFNQLQPPFNNEKLRQAMLYIV
                     DQQDVMTTLAGDPANWKTCYSVYTCGTPMASEAGAAPLMGKRDLAKAKELVKESGYNG
                     ERIVLLSATDQPIVNAQANVVAAEMREIGLNVDLQAMDWGTLITRRASREPVDKNGWS
                     VFFTWFVGPDMLNPALSFPLRANGEKAWFGWPTDDKLEALHAQWMEAPDLAAQQKIAQ
                     RLQEEAFASVPFVPTGQFVIPTAFRKNLKGVAIAPVVFLWNVEKN"
BASE COUNT          822 a         1443 c         1602 g          681 t
ORIGIN      
        1 ctgccgagag cagcgcctcg gtatagggat gaaggggcgc ccggaagagt gcgtccttct
       61 ccgcgagctc gacgatcttt ccgagataca tgacggcgac gcggtggctg atgtgctcga
      121 ccaccgcgag atcatgcgca atgaagaggt aggcgagctt cagctcctgc tgcaggtccc
      181 gcagcagatt gacgacttgc gcctggacgg agacgtcgag cgccgagacg ggctcgtcga
      241 gcacgatgag gctcggattg agcaccagag cgcgggcgat gccgatgcgc tggcgctggc
      301 cgccggagaa ctcgtgcgga tagcgccgca tcgcctcctc gcgcaggccc accttggcga
      361 agatctcggc gacgcgctgc tcgagcgcct tgccgcggaa gacgccgtaa ttcgccagcg
      421 gctcgcccac gatctcgccc accgaaagcc gcgggttgag cgaagaataa ggatcctgga
      481 acaccacctg cagctcgcgc cggtagggcc gcatggcgcg gcggtcgaga tggtcgatgc
      541 gctcgccgcg gagcttcacg gtgccggcgg tcggatcgat gagcttcagg atcgcctttc
      601 ccgccgtcga cttgccgcag ccgctctcgc cgacgaggcc cagggtctcg ccaggggcga
      661 tgtcgaagct gatgccatcg acggcgtgga cgtagccgac gacgctcgcg agcacgccct
      721 tgtgcaccgg gaaatgcttc ttgagccctt cgacctccag tagaggcagc ggtgcgctca
      781 tgcggcgaac cgcgcgctct cgaagcaggc ggcgtagtgg cccgggttct tctcctcgag
      841 cgggggcgcc gcgcggcggc agcgctcgac ggcgaaggcg cagcgcggcg caaaggcgca
      901 gccccgtatc tcctcgcgca gggacggcac cacgccggga atctccggaa ggcgaggcac
      961 gtccgttcgg ccgatggtgg cgccgagccg cggcacagaa gcgagcagcc ccagcgtgta
     1021 gggatgacgc ggcttaagga agagatcgag tacctgcgcc tcctcgacct tccgcccggc
     1081 atacatgacg acgacgcgcg acgccatctc cgcgacgacg ccgagatcat gggtgatgag
     1141 gatgatggcg gcgccgcttc gcgccttcat cgtgcgcatc agatcgagga tctgcgcctg
     1201 aatggtgacg tcgagcgcgg tggtcggttc gtcggcgatc aagagcttgg gattgcaggc
     1261 gagcgccatg gcgatcatca cgcgctgacg catgccgccc gagagctcgt gcggatattg
     1321 ccgcgcgcgg cgctcgggct cggaaatgtt gacgaagcgc agcatctcga cggtgcgcgc
     1381 cgccgcggcg gcacggtcga ggccctgatg cagcatcagc gtttcgccga tctggtgcga
     1441 gatacggagc accggattga gcgaggtcat cggctcctgg aagatcatgg cgatctcgtt
     1501 gccgcggatg ctccgcatct cggcctcgct cgcctcgaga agatcgcgcc cggcgaagcg
     1561 gatggcgccg ccgacgatgc ggccgggcgg cgacggcacg agccgcagga tcgagagcgc
     1621 agtgacgctc ttgccacaac cgctttcgcc caccaccgcc agggtctcgc cagcgccgac
     1681 gtcgtaggag acgccgtcca ccgcgcgcac gacgccgtcg cgggtgaaga aatgcgtgcg
     1741 caggtccgcg atggcgagga gcggctcggg cgcgccgctc acagccgccg cgccagccgc
     1801 gggtcgagcg cgtcgcgcaa gccgtcgccg accaggttca ccgccagcac cgtcagcgaa
     1861 aggaaggcgc ctgggaagag caccagatag ggcttgatct gaaaatagct gcggccttcc
     1921 gccatcatat tgccccagct cgggatctcg ggcgaggtac cggcaccgat gaagctcagc
     1981 accgcctcgg tgatcatcgc cgcggcgcag atgtaagtag cctgcaccag cagcggcgcg
     2041 atcgtgttgg ggaggatgtg gcggagcaaa gtgcgccaga atccggtgcc cgccgccacc
     2101 gccgcttcga cgaaaggttg ctcgcggagc gaaagcacga cgctccgcac cagccgtgcc
     2161 acgcgtggca cctcggtgat ggtgatggcg aggacgacgt tgccgaggct cgccttggtg
     2221 agcgccatga gcgcgatcgc gagcagcacc ggcggcaccg acatgagggc gtccatgacg
     2281 cgcatcacca cgaagtcgag gcggcgcacg aagccggtga cgagaccgat ggcaagcccg
     2341 agcgcggtgc cgaacatcgc caccgagaga ccgacggtga gcgagacgcg ggcgccgtag
     2401 agcgtgcggc tgtagacgtc gcggccgtag ctgtcggtcc cgaaccagta gagctcggac
     2461 ggcggccgga gacgcttcac cggcgccacc gccagcggat cgacggtgcc gagatagggc
     2521 gcgagcagcg ccatcgccgc catcgccagg agcagcatga cgccgatcat caccgtcgga
     2581 tgccgccgcg ctgtctcgcc aagccaatgg cgctggacga tcggggcgat ctcggcgggc
     2641 gccagcgcca tgctagaacc gcacgcgggg atcgaagaag gtgtagctca cgtcaacgag
     2701 aagattgata acgacgtaga ccgccgagag gaccaaggtc acgccctgga tgatcgggta
     2761 gtcgcggcgg aggatggcgt cgaccgtgag ccggccgatg ccgggaatgg cgaagaccgt
     2821 ctccgtgacg atgacgccgc cgatcagcag cgcgatgccg ctgccgatca cggtgacgat
     2881 cgggatcgcc gcgttgcgca gcgcgtggcc gaccagcacc ttgccctcgg taaggccctt
     2941 ggcctgcgcc gtgcggatat agtcctggct cagcacctca agcatggcgg cgcgggtgat
     3001 gcgggcgatg agagcgccat agatgaggcc gagcacgatg ctcggcagga tgagatgctc
     3061 gatgaagggc gcgaagccct cgctgaggct ggcgtagccc tggaccggaa gccagtcgag
     3121 ccagaccgag aagcagagga tggcgatata ggcgaggacg aaggacggca cggagaatcc
     3181 cagcacggct acgaccatga caagacgatc gatccagctc ccgaccttcg acgccgcgat
     3241 gacgcccaag ggaatggcga agctcagggt aatgatgaga gcaattaccg tcagcgacac
     3301 ggtcggtccg acgcgctgcc cgatgagcgt cgtcacaggg aggttggtga agatcgaggt
     3361 cccaaggtcg ccctgcagca cgccccacgc ccaatggccg aagcgcacga ggaagggatc
     3421 gtcgagcccg agctggcgat ggatgcgcgc gatgtcgtcg gtcgtcgcga gatcgccggc
     3481 gatcgtcgcc gcgggatcgc ccggcgtcag atagagcagc gagaacacga agaacgccac
     3541 gaaggtcatg acgaagacag tggcgacgag gcggcggacg acgtaagcga gcatgtgcta
     3601 ggccgtccgc gcgttcgggg gactcgtcac gaaatcataa ttgccgttcc cgcgaaagcg
     3661 ggaacccaga gcttcaggtc ttcgtcgctc gccctggttc cccgctcgcg cggggaagga
     3721 agccgcggct ggtttcatcg cgcaataccg aagcgctaat tcttctccac attccacagg
     3781 aagaccaccg gtgcgatcgc gacacccttc aggttcttgc ggaaggccgt cgggatgacg
     3841 aattgcccgg tcggcacgaa cggcacgctg gcgaaggctt cttcctggag ccgctgcgcg
     3901 atcttctgct gcgcggcgag gtcgggcgcc tccatccatt gcgcgtggag tgcctcgagc
     3961 ttgtcgtcgg tgggccagcc gaaccacgcc ttctcgccgt tggctcggag cgggaagctg
     4021 agcgcgggat tgagcatgtc gggaccgacg aaccaggtga agaacacgct ccagccgttc
     4081 ttgtcgacgg gctcgcggct ggcccggcgc gtgatgagcg tcccccaatc catcgcctgc
     4141 aggtcgacgt tgaggccgat ctcgcgcatc tctgcggcga cgacgttggc ctgcgcgttg
     4201 acgatcggtt ggtcggtggc ggagagcagc acgatgcgct cgccgttgta gccgctctcc
     4261 ttgacgagct ccttggcctt ggcgaggtcg cgcttgccca tgagtggcgc cgcgcccgcc
     4321 tcgctcgcca tcggcgtgcc gcaggtgtag acggaatagc aggtcttcca gttcgcggga
     4381 tcgccggcga gcgtcgtcat cacgtcctgc tggtcgacga tgtagagcat cgcctggcgg
     4441 agcttctcgt tgttgaaggg cggctgcagc tggttgaagc gcatgacgcc gatgctgccc
     4501 aagggatcga cgttggcgac ggtgatgtcc ttgttccgct gcagctgc
//
LOCUS       DASYNS010000409         4167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1195133, whole genome shotgun sequence.
ACCESSION   DASYNS010000409 DASYNS010000000
VERSION     DASYNS010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195133"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VGP50_12670"
     CDS             complement(<1..617)
                     /locus_tag="VGP50_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8028279.1"
                     /translation="MSDETMVARLFAAALEHQKRGDREPALKAYRRVVEIAPERADAW
                     NNLGILLGKQGKLRAAIACLKRAIAVPAPSAVHYSNLGEFLRRALRFDEATAAFRQSL
                     ALEPERPETLHNYGLLLRDMTDTPGAVAIFNRLLERAPADASLRWERALALLAAGDLP
                     QGFAAYESRWQAKATMPPFPMPLWQGEPLDAKSILVHAEQGFGDM"
     gene            complement(625..1884)
                     /locus_tag="VGP50_12675"
     CDS             complement(625..1884)
                     /locus_tag="VGP50_12675"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8028280.1"
                     /translation="MAEKHSEAEQLSRAALQLLEQGKVEAAAACMKRAAAASPTSAET
                     WCNLSIALRMAQRFEEARDAIDRALTLDPDRPETVESYGILLHNMGDGKGAIRALDRA
                     LELSPGNAKTRWNRAIVALANGDLRAGFADHEARREIAELAPRRFPMPSWSGERISGR
                     TILVHAEQGFGDTLQFARYLPMLAGKGARVIFAVQPELLRLFQNAPGLAGVISIDGKL
                     PLTDFHVPLLTLPLRFATTLDDVPATVPYITPPQGLQATLVRAPDTRLAVGIAWAGRP
                     THKYDRDRSMAIEEFLALSDLPGVALYSLQTGARSQDLAASGAGVLIRDCSAALGDFA
                     DTATALTQIDLVVCVDTALAHLAGAMGRNAFVLLPFVADWRWLRQRQDSPWYPTLRLF
                     RQEKPGDWKSVIRRVREAVADLLGPRA"
     gene            complement(1957..>4167)
                     /locus_tag="VGP50_12680"
     CDS             complement(1957..>4167)
                     /locus_tag="VGP50_12680"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8028281.1"
                     /translation="MGLLAAQAGRPDLAIQHLRETIARGPEAATYHLHLGMILSGLGR
                     LDEAEACYRTALRLAPDYPEAHNNLANALGQQGRLGDAEIHYRETLKLRPDLPEAYNN
                     LGHVLNCLGRHAEAEITLRRALALRPNSVAALGNLGLALTALGRFAEAEASYRSALGL
                     RADIPELQRNLGNLLAKLRRLSEAADCYRATLRLKPDDAQAYKAMGDLFANASRCEEA
                     AICYREAVRFDATLDDARNNLGLMIQGLGRHDEAIVHYDAVLGRSPGFIAARINRCIA
                     RLLVVYRDEAELVNARALFTEELEAICRLPAGTVSSDAIAGATPFYLAYQGRPDRDLQ
                     ARYGRFVADVMAAHYPAWTSPPAVPPPQPGEPIRVGFLSACFYSHSNWKIPIKGWLTG
                     LDKQRFQIFGYHLGDTTDSETSIAKGLCHRFVQGMPSLEAWAETIRADRLHVLIIPGI
                     GLDGVTHRLAALRLAPVQATSWGHPDTTGLPTIDHYLSSDLMEPPDGDAHYTERLIRL
                     PNLSIAYAPPAPAPAAMSRGDIGVPEDATLYWCCQSLFKYLPRHDWIFARIAAAVPEA
                     RFVFIKYPRGDAVNAIFRERLGAAFAAAGLDATRHCIFMPPLDSPRFAAIGKMADLFL
                     DSLGWSGCNSTLEALALDLPVIAMAGDLMRGRHSAAILTMLGMPELIAPTPEAFAALA
                     IRLGRDGPARQALRARIAQEKHRLYRDQAAIDGLARYLEVAAREPASTASEPAG"
BASE COUNT          658 a         1348 c         1519 g          642 t
ORIGIN      
        1 atcatgtcgc caaagccctg ctccgcgtga accaggatcg acttggcgtc gagcggctcg
       61 ccctgccaca gcggcatcgg aaacggcggc atggtcgcct tggcctgcca gcgggattcg
      121 tacgccgcaa aaccttgcgg caggtcgccc gcggcgagca gcgccagcgc gcgctcccag
      181 cggagcgacg cgtctgccgg cgcgcgttcg agcaggcgat tgaagatcgc gacggcgccc
      241 ggcgtgtccg tcatatcgcg cagtaacagg ccgtaattgt ggagcgtctc cgggcgctcg
      301 ggctcgagcg cgagcgattg ccggaacgcc gccgtcgcct cgtcgaaccg caaggcgcgg
      361 cgcaggaatt cgccgaggtt cgaataatgg acggccgacg gcgccggcac cgcgatcgcg
      421 cgcttgagac aggcgatggc ggcccgcaac ttgccttgct tgcccagcaa tatgcccaaa
      481 ttgttccagg cgtcggcccg ctctggcgcg atctcgacga cgcggcgata ggctttgagc
      541 gccggctcgc ggtctccgcg tttctgatgc tcgagggcgg ccgcgaagag ccgcgccacc
      601 atagtctcgt ccgacatcga ctaactaggc ccgaggcccg agcagatcgg cgaccgcttc
      661 gcgcacgcgg cggatgacgc tcttccagtc gccgggtttc tcttggcgga agagccgcag
      721 cgtcgggtac cagggactgt cctgccgctg gcggagccag cgccagtcgg cgacgaaggg
      781 aagcagcacg aaagcgttgc gccccatcgc gcccgcgaga tgcgcaagcg cggtgtcgac
      841 gcacacgacg agatcgatct gcgtcagcgc cgtcgccgtg tcggcgaaat cgccgagcgc
      901 ggcgctgcaa tcgcggatga ggacgcccgc gccgctcgcg gccaggtcct gcgaccgtgc
      961 cccggtttgc aggctgtaga gggcgacgcc cggcagatcg ctcaaggcga ggaattcctc
     1021 gatcgccatc gatcgatcgc ggtcgtattt gtgggtcggc cgcccggccc aggcgatacc
     1081 cacggcgagc cgcgtgtcgg gcgcgcgcac gagcgtcgcc tgcaggccct ggggcggcgt
     1141 gatgtagggc accgtcgccg gcacgtcgtc gagcgtcgtg gcgaagcgca gcggcagggt
     1201 cagcagcggc acgtggaagt cggtgagcgg cagcttgccg tcgatcgata tcacgcccgc
     1261 cagccccggc gcattctgga agagacgcag caattcgggc tgcacggcga agatcacgcg
     1321 cgctcccttg ccggccagca tcgggagata tcgcgcgaat tgcagcgtat cgccgaagcc
     1381 ttgctcggcg tggacgagaa tggtgcggcc ggagatgcgc tcgccgctcc atgacggcat
     1441 cggaaagcgc cgcggcgcca gctcggcgat ctcgcgccgc gcctcgtgat cggcaaagcc
     1501 ggcgcggaga tcgccattgg cgagcgcgac gatggcacgg ttccaccggg tcttggcgtt
     1561 cccgggcgag agctcgaggg cgcggtccag cgcgcgaatg gcgcctttgc cgtcgcccat
     1621 gttgtgcagc aggatgccgt agctctccac ggtctccggg cgatcgggat cgagggtgag
     1681 cgcgcggtcg atggcgtccc gcgcctcctc gaagcgctgc gccatgcgca gcgcgatcga
     1741 aagattacac caggtttccg cggaggttgg tgacgcggcg gcggcgcgct tcatgcaagc
     1801 ggcggccgcc tccaccttcc cctgctcgag gagctggagc gccgcccggc tcagttgctc
     1861 ggcctcgctg tgcttctcgg ccatggccga tggtaggcgg ttcgatctcc gccagcaacg
     1921 gacgaagcag ccgctaaatg ccgcgcgcga ggacgctcag cccgccggtt ccgaggctgt
     1981 ggaagcgggc tcgcgcgcgg caacctcgag gtagcgggcg aggccgtcga tcgcggcctg
     2041 gtcgcgatag agacggtgct tctcctgggc gatgcgcgcg cgcagcgcct gtcgcgccgg
     2101 gccatcgcgg ccgaggcgga tcgccagcgc ggcgaaggcc tcgggcgtcg gggcgatgag
     2161 ctcgggcatt cccagcatcg tcaggatggc ggcgctgtgc cggccgcgca tgagatcgcc
     2221 ggccatggcg atgacgggga gatcgagcgc cagcgcctcg agcgtcgagt tgcagccgga
     2281 ccagccgagg ctgtcgagaa acaggtcggc catcttgccg atcgcagcga aacgcgggct
     2341 gtcgagcggc ggcatgaaga tgcaatgacg ggtggcgtcg agaccggcag cggcaaaagc
     2401 agcgccgagg cgctcgcgga agatggcgtt cacggcgtcg ccgcgcgggt atttgatgaa
     2461 cacgaagcgc gcctcgggca ccgccgcggc gatccgtgcg aatatccagt cgtggcgcgg
     2521 cagatacttg aacagggact ggcagcacca atagagcgtc gcgtcctcgg gcacgccgat
     2581 atcgcctctc gacatcgcgg ccggcgcggg cgccggcggc gcataggcga tggacaggtt
     2641 ggggaggcgg atcaggcgct cggtgtaatg cgcgtcgccg tccggcggct ccatcagatc
     2701 gctgctgaga tagtggtcga tggtcggcag gcccgtggtg tcgggatgtc cccatgaggt
     2761 cgcctgcacc ggcgccaggc gcaaggccgc caggcggtgc gtcacgccat cgaggccgat
     2821 gccggggatg atcagaacgt gcaggcggtc ggcgcggatc gtctcggccc aggcttcgag
     2881 cgacggcatg ccctggacga agcggtggca gagtcctttg gcgatcgacg tctccgaatc
     2941 cgtggtatcg ccgaggtgat agccgaagat ctgaaaccgc tgcttgtcca gtccggtcag
     3001 ccagcctttg atcggaatct tccagttgga gtggctgtag aagcacgccg agagaaagcc
     3061 gacgcggatc ggctcgcccg gttgcggcgg cggcactgcg ggcggcgatg tccaggcggg
     3121 atagtgggcg gccatgacat cggcgacgaa gcggccgtag cgcgcctgga ggtcgcggtc
     3181 cgggcgtcct tgataggcca gatagaatgg cgtcgccccg gcgatggcgt cgctgctgac
     3241 ggtgccggcc ggcagccggc agatcgcttc cagctcttcg gtgaagagcg cccgcgcgtt
     3301 cacgagctcg gcctcgtcgc gatagacgac cagcagccgg gcaatgcagc gattgatccg
     3361 cgccgcgata aaaccgggac tgcgcccgag caccgcgtca taatgcacga tggcctcgtc
     3421 atggcgaccg aggccctgga tcatcagtcc gagattgttg cgcgcgtcgt cgagcgtcgc
     3481 gtcgaagcgc accgcttcgc gatagcagat cgccgcctcc tcgcaccggc tcgcatttgc
     3541 gaacaagtcg cccatcgcct tgtaggcttg cgcatcgtcg ggcttgaggc gcagcgtcgc
     3601 gcgatagcaa tcggccgcct cgctcaggcg gcggagcttg gccaggaggt tgccgagatt
     3661 gcgctgcagc tccggtatgt cggcgcgcag gccgagggcg ctgcgatagc tcgcttccgc
     3721 ctcggcgaaa cggccgagcg cggtcagcgc gaggccgaga ttgccgaggg ccgcgacgga
     3781 gttcggccgg agcgcaagcg cgcgccgcag cgtgatctcc gcttcggcat gccggccgag
     3841 gcaattcagc acatggccga gattgttgta agcctcaggc aagtccggcc tcagcttgag
     3901 cgtctctcgg tagtggattt cggcgtcgcc cagccgtccc tgctggccga gcgcgttggc
     3961 gagattgttg tgcgcctcgg gataatcggg agccagacgt agcgcggtgc gatagcacgc
     4021 ttcggcctcg tcgaggcggc cgaggccgct caggatcatg ccgagatgaa ggtggtaggt
     4081 ggccgcctcg gggccgcgcg cgatcgtctc tctgaggtgc tggatcgcga gatccgggcg
     4141 tccggcttgc gccgcgagca atcccat
//
LOCUS       DASYNS010000410         3545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5095448, whole genome shotgun sequence.
ACCESSION   DASYNS010000410 DASYNS010000000
VERSION     DASYNS010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5095448"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VGP50_12685"
     CDS             <1..255
                     /locus_tag="VGP50_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8028282.1"
                     /translation="YYRLGDFTKATQTLEHAIELLPEDPTINDHLGDAYWQAGRVAEA
                     RFQWRRALQFKPEAGDVKTIEGKLDRGLAKLPAAAAMRGG"
     gene            255..1109
                     /locus_tag="VGP50_12690"
     CDS             255..1109
                     /locus_tag="VGP50_12690"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEV8028283.1"
                     /translation="MALRRFAPAKLNLYLHVVGRRDDGYHLLDSLVAFADVGDEIIAA
                     PAARLSLAVTGPYAAALAGAPDDNLVWRAAERLAAAAGRPAAAALTLVKNLPVASGIG
                     GGSSDAAATLKALAELWHVDIAPADLMTLAASLGADVPVCLAGRAAWLGGVGERIEPV
                     AGLPTATVLLVNPGVALPTAAVFAARRGPFSAPARFSLPAGAVELARLLEQRRNDLTA
                     PAIERVPAIGRVLDRLAAAEGALIARMSGSGATCFALFTAPEAAAAASARLAAEQPDW
                     WVAAGRLF"
     gene            1299..1511
                     /locus_tag="VGP50_12695"
     CDS             1299..1511
                     /locus_tag="VGP50_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028284.1"
                     /translation="MGKLEDAVARLERAIARLEQAVDRAAHDERSEQAALLAARAEYA
                     ALSAVSDGVAARLDAAIGRLDRVLEG"
     gene            1511..1834
                     /locus_tag="VGP50_12700"
     CDS             1511..1834
                     /locus_tag="VGP50_12700"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV8028285.1"
                     /translation="MTQVTVTINGRSYPIACKEGEEERIGELARYIDGKMRNFAQEFG
                     NVGETRLFLLTALVLADELADAQAASGRARAANGAADGKLATGIDNLAMRLEAIAAKL
                     ETSHI"
     gene            1842..1997
                     /gene="ssrS"
                     /locus_tag="VGP50_12705"
     ncRNA           1842..1997
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VGP50_12705"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2024..2860
                     /locus_tag="VGP50_12710"
     CDS             2024..2860
                     /locus_tag="VGP50_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEV8028286.1"
                     /translation="MRLLLCGDVVGRSGRTVVTTQVPRLRRELALDFVVVNGENAAHG
                     FGITDKICEEFYASGVDVITTGNHVWDRRDIISYIDGDPRLLRPVNFPPGTPGKGHGV
                     FALGDGRSVMVINAMARLFMDAIDDPFTGVERLLAERPLGRVNAILLDFHGEATSEKM
                     SMGHFCDGRVSAVVGTHSHVPTADAQILPKGTAYMTDAGMCGDYDSVIGMQKEAAIAR
                     FLRKMPGDKLQVAEGEATLCAAFIETDDATGLATRIAPLRLGGRLKAHWPLDVPARAF
                     IP"
     gene            2905..3186
                     /locus_tag="VGP50_12715"
     CDS             2905..3186
                     /locus_tag="VGP50_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028287.1"
                     /translation="MDRDSGEPGRGASQRRAIATAGLLIVASLIAIGLYAWFSFSDMA
                     MTTSGYIALALGIAGTMALAIGLMSLVFFSNRFGYDEQVGGGDKQKGPE"
     gene            3243..>3545
                     /locus_tag="VGP50_12720"
     CDS             3243..>3545
                     /locus_tag="VGP50_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEV8028288.1"
                     /translation="MVASPLPLALYGFALGWSVAWPPGPINAEIVRRGLARGFWAACG
                     LGLGAASGDTIWAAAVALGAGALLDFARLRLLLSIVSSALLLLLGALFLRRAARGAA"
BASE COUNT          521 a         1179 c         1248 g          597 t
ORIGIN      
        1 tactaccggc tgggcgactt caccaaggcg acacagacgc tcgagcatgc aatcgagctg
       61 ctgcccgagg atccgaccat caacgatcac ctgggcgacg cctattggca ggcggggcgg
      121 gttgcggagg cgcgcttcca atggcgtcgc gcgctgcagt tcaagcccga ggccggcgac
      181 gtcaagacga tcgaaggcaa gctcgaccgc ggccttgcca aattgccggc ggccgcggcg
      241 atgcgcggcg gctgatggcg ctgcgccgct tcgctccggc gaagctcaat ctctatctgc
      301 atgtcgtcgg ccgccgcgat gacggttatc acttgctcga cagtctcgtc gcgttcgccg
      361 atgtgggcga cgaaatcatt gccgctcccg ccgcgcgatt gtcgctcgcg gtgacggggc
      421 cgtacgcggc ggcgctcgcc ggtgcgcccg acgacaatct ggtctggcgg gcagccgagc
      481 gtcttgccgc cgcggcgggc cggccggcgg cggcggcact caccctggtc aagaacctgc
      541 cggtggcttc gggcatcggc gggggctcga gcgacgcggc ggcgacattg aaggcgctcg
      601 cggaactctg gcacgtcgac atcgctccgg ccgatctcat gaccctcgcg gcgtctctcg
      661 gtgccgacgt gccggtctgc ctcgccggac gcgcggcgtg gctcggcggc gtcggcgagc
      721 ggatcgagcc ggtcgcaggc ctgccgacgg cgacggtgct cctcgtcaat cccggtgtcg
      781 ctcttccgac ggcagcggtg tttgcggcgc ggcgcggacc tttctctgcg ccggcgcgct
      841 tctcgctgcc ggccggcgcc gtcgagctgg cgcggctgtt ggaacagcgc cgcaacgact
      901 tgaccgcgcc ggcgatcgag cgtgtgccgg cgatcggccg cgtgctcgat cgtcttgcgg
      961 cggccgaagg cgcgctcatc gcgcgcatga gcggcagcgg ggccacctgc tttgccctct
     1021 tcaccgcgcc cgaagccgcc gccgctgcaa gcgcccggct cgcggcggag caacccgact
     1081 ggtgggtcgc ggcgggacgg ctgttctagg acgtctgttt ctgccgggca gaacgggcca
     1141 ccgacggttc tcccgcattc agagagccgt cagtcgaccc atcacttcga cagtgcgccc
     1201 acgattttcg tgcacgcgac gcgcgtttgc gccgcgattg acggcccgtt tgctgcgcgc
     1261 gtaaggtgtc gagtcgttcg aaggggggcc gagaaggcat gggcaagctg gaggacgcgg
     1321 tggcgcgcct ggagcgagcg atcgcgcggc tggaacaggc cgtcgaccgc gccgcacatg
     1381 acgagagatc cgagcaggcc gcgttgctcg ccgcccgagc cgaatacgcc gcgttgtcgg
     1441 cggtgagcga tggcgtggcc gcgcgtctcg atgcggcgat cggtcgcctc gaccgcgtgc
     1501 tggagggctg atgacgcagg tgaccgtcac catcaacgga cgcagctacc cgatcgcatg
     1561 caaagagggc gaagaggagc ggatcggcga gctcgcgcgc tatatcgacg gcaagatgcg
     1621 caactttgcc caggaattcg gcaatgtcgg cgagacgcgg ctgttcctgc tgacggcact
     1681 ggtgctggcc gacgaactgg ccgacgccca ggcggcgagc ggccgggcgc gtgccgccaa
     1741 tggcgccgcc gacggcaagc tcgccacggg catcgataat ttggcaatgc gcctcgaggc
     1801 gattgcggcg aagctcgaaa cctcccacat atagggcgag gatggggctg cacggtgcgt
     1861 taggtcgcac ataccccggg gccgatatcg acctctaggg agctgtccct gccggagccc
     1921 tggcttcggt atatggctcc cacctgcgtt agcgggccac gagggtcatg catgccgacg
     1981 gccgcggcgg ctccatcctc acttgggtca tgaaggaaca agaatgcggc ttcttctctg
     2041 cggcgatgtg gtgggacgct ccgggcgcac cgtcgtcacc acgcaggtgc cgcggctgcg
     2101 tcgggaactg gccctcgatt tcgtcgtcgt caatggcgag aacgccgcac atggcttcgg
     2161 catcaccgac aagatctgcg aggaattcta cgcgagtggc gtcgatgtga tcacgacggg
     2221 caaccacgtc tgggaccggc gcgacatcat ctcgtatatc gatggcgacc cgcggctgct
     2281 gcgacccgtc aacttcccgc cgggcacgcc gggcaagggc catggagtct tcgcgttggg
     2341 cgacggccgc agcgtgatgg tgatcaacgc catggcgcgg ctcttcatgg acgccatcga
     2401 cgatcccttc accggcgtcg aacgactgct cgccgagcgc ccgctcggcc gggtcaatgc
     2461 gatcctcctc gattttcacg gcgaagcgac gtccgaaaaa atgtcgatgg gccatttctg
     2521 cgacggccgc gtgtcggccg tggtgggcac gcacagccat gtgccgaccg ccgatgcgca
     2581 gattctcccc aaaggcacgg cctacatgac ggacgcgggc atgtgcggcg attatgattc
     2641 cgtgatcggg atgcagaagg aggcggcgat cgcgcgcttc ctgcgcaaga tgccgggcga
     2701 caagctccag gtcgccgaag gcgaagcgac actctgcgcc gctttcatcg agaccgacga
     2761 tgcgacgggt ctcgcgacgc gcatcgcgcc cctgcggctc ggcggccggc tcaaggcgca
     2821 ttggccgctc gacgtgccgg cgagggcttt cattccctga aggccgcgca gaccggctcg
     2881 ttcgcacagg gaggtggatc gccgatggac cgagattctg gcgagcctgg acgaggtgcg
     2941 tcgcagcgcc gggcgatcgc gaccgccggc ctcctgatcg tcgcctcgct gatcgcgatc
     3001 ggcctctatg cctggttctc cttcagcgac atggcgatga cgacgagcgg gtatatcgcg
     3061 ctcgcgctcg gcatcgccgg cacgatggcg ctcgcgatcg gcctcatgag cctcgtcttt
     3121 ttcagcaatc gcttcggcta tgacgagcag gtcggaggcg gcgataagca gaagggaccc
     3181 gaatagcgcc acgccagcca tgctagggag catggcgctc gtcctttggg gcaatgcacg
     3241 agatggtcgc gtcgccgttg ccgctcgcgc tctacggttt tgccctgggc tggtccgtcg
     3301 cctggccgcc gggtcccatc aacgccgaga tcgtccgtcg tggtctcgcg cgcggcttct
     3361 gggcggcttg cggcctgggt ctcggtgccg cctcgggcga tacaatctgg gccgcggcgg
     3421 tggcgctggg cgccggggcg ctgctcgatt tcgcccggct ccggctgctg ctctccatcg
     3481 tcagttcggc actgctgctc ttgctcggag cgttgttcct gcgccgcgcg gcacggggcg
     3541 ccgcc
//
LOCUS       DASYNS010000411         4975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1195857, whole genome shotgun sequence.
ACCESSION   DASYNS010000411 DASYNS010000000
VERSION     DASYNS010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195857"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="VGP50_12725"
     CDS             complement(<1..1197)
                     /locus_tag="VGP50_12725"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272054.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEV8028289.1"
                     /translation="MRVERRFTVAEQDPYGSIEFRRATSEIRNPDGSTVFHLPDIEVP
                     AAWSQVACDILAQKYFRKAGVPAKLGPVVEADVPDWLWRRTADQAALAALPADERFGS
                     ERSAKQVFDRLAGTWAYWGWKGGYFDREADAQAYYDETRFMLARQMAAPNSPQWFNTG
                     LHWAYGIDGPAQGHYFVDFRTGAVKASDSAYEHPQPHACFIQSVADDLVNEGGIMDLW
                     VREARLFKYGSGTGSNFSKLRGENEKLSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRR
                     AAKMVTVDIDHPDIEAYIDWKVLEEQKVAALVAGSKLAQKHLNLVMAACHSLEAQDLK
                     DGRFDPARNAALKREIKAARRAMIPENYVQRVIQFAEQGYTEIDFRAYDTDWDSDAYL
                     TVSGQNS"
     gene            1531..1908
                     /locus_tag="VGP50_12730"
     CDS             1531..1908
                     /locus_tag="VGP50_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEV8028290.1"
                     /translation="MNLGTSLYTWLHGELVGTDQFGNRYYREKRRRALKRGGGIESRE
                     RRWVLYNGEVEASRVPPLWHGWLHHIFDDVPDEAARKKYPWEKEYVPNLTGTDRAYRP
                     PGSLLRGGHRQRAAGDYEPWKPE"
     gene            1945..2460
                     /gene="mlaD"
                     /locus_tag="VGP50_12735"
     CDS             1945..2460
                     /gene="mlaD"
                     /locus_tag="VGP50_12735"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEV8028291.1"
                     /translation="MNQNAVETVMGAVVLVVAAVFLFFAYSTSRPPSLSGYDVTAKFD
                     RVDGLRDGGDVRISGIKVGTIVSQTLDPQTYLAVVHMTIDNSIKLPVDTIATITASGL
                     LGDNYLALVPGGDEDYIKPNGQITHTEAAINLFGLINKYIGSGANGNGAAPGPAPPPA
                     QNKGGQKAPSK"
     gene            2567..3025
                     /locus_tag="VGP50_12740"
     CDS             2567..3025
                     /locus_tag="VGP50_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990531 - phospholipid-translocating
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005548 - phospholipid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEV8028292.1"
                     /translation="MVNRNAFETAMGGVVLLGAAVFMLFVYTSTRSPAMSGGYNVVAR
                     FTRVDGLRDGSDVRVGGIKVGTVTSQRLDPRTRIPEVRMTIDTALQIPTDTMATIAST
                     GLLGENYVDLELGNDEEQIKADGVIARTEAAINIFSIISKYLSCASRNPT"
     gene            3022..3264
                     /locus_tag="VGP50_12745"
     CDS             3022..3264
                     /locus_tag="VGP50_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028293.1"
                     /translation="MTSTSPHQKLVLPRWLTPRGEPVSCIDKIKVLNKNLEELRDMAQ
                     EAFEDAVLMGCDEAQFRGVLHQLADGLVNPYGKDRP"
     gene            3261..3668
                     /locus_tag="VGP50_12750"
     CDS             3261..3668
                     /locus_tag="VGP50_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HEV8028294.1"
                     /translation="MKRHFLGLAAALIVAATGARAETVAVLQGLDKTTARISKIEAPV
                     GKSVRLGTLVITVRECVKHPPEEEPESAAFLQIDEMRQGDPTTAVSERVFSGWMFASS
                     PALSALENPIYDIGVLDCKSDATAAPASGSRGK"
     gene            complement(3596..4249)
                     /gene="aat"
                     /locus_tag="VGP50_12755"
     CDS             complement(3596..4249)
                     /gene="aat"
                     /locus_tag="VGP50_12755"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEV8028295.1"
                     /translation="MTVLTPDLLLRAYAAGIFPMAESADDPDVFWVDPEQRGILPIEG
                     FHLPRRLRRRLRQDAFELRCNCAFEAVVRGCAEPRAERPKTWINEEIVQLYAALFRTG
                     FAHSVEAWREGELVGGLYGVALGGAFFGESMFSRVSDASKIALAHLVARLRLGGFRLL
                     DTQFVTEHLQQFGAIEIARGQYHRRLAQALTVKAYFPREPLAGAAVASLLQSSTPMS"
     gene            complement(4374..>4975)
                     /locus_tag="VGP50_12760"
     CDS             complement(4374..>4975)
                     /locus_tag="VGP50_12760"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614313.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV8028296.1"
                     /translation="ADARAQIGNTAVAAMAEIGYAGAGTLEFLYQDGEFFFIEMNTRI
                     QVEHPISEMVSGIDLVREQIRIAAGATLGYRQEDIELRGHAIECRINAENSQTFMPSP
                     GRVTSYLAPGGLGVRVDSALYQGYTVPPNYDSLVAKLVVYGTTRNECLMRMRRALQEY
                     VIAGIDTTIPLHQRLVAAPDFINGAYDIHWLEGFVAAIG"
BASE COUNT          893 a         1597 c         1644 g          841 t
ORIGIN      
        1 ggagttttgg cccgagaccg tgagataggc gtcgctgtcc cagtccgtgt cataggcgcg
       61 gaaatcgatc tcggtgtagc cctgctcggc gaactggatg acgcgctgga cgtagttctc
      121 ggggatcatt gcgcggcgcg ctgccttgat ctcgcgcttg agcgcggcgt tcctcgccgg
      181 atcgaagcgg ccgtccttga ggtcttgcgc ctcgagcgag tggcaggccg ccatcaccag
      241 gttcagatgc ttctgcgcca gcttcgatcc ggcgaccagc gccgcgacct tctgctcctc
      301 gagcaccttc cagtcgatat aggcttcgat gtcggggtgg tcgatgtcga cggtcaccat
      361 cttggcggcg cgccgcgtcg tcccgccgga cttgatggcg cccgccgcgc ggtcgccgat
      421 cttcaagaag ctcatcaacc ccgaggagcg gccgccaccc gacagcttct cgttctcgcc
      481 gcgcagtttc gagaaattgg agccggtgcc cgacccatat ttgaagagcc gcgcctcgcg
      541 cacccagaga tccatgatgc cgccctcgtt gacgaggtcg tcggcgacgg attggatgaa
      601 gcaggcgtgc ggctgcgggt gctcgtaggc gctgtccgac gccttcaccg cgccggtccg
      661 gaaatcgacg aagtagtggc cttgcgccgg cccgtcgatg ccgtaggccc agtggagccc
      721 ggtgttgaac cattgcggcg agttgggcgc cgccatctgc cgcgccagca tgaaacgcgt
      781 ctcgtcgtaa taggcttgcg cgtcggcttc gcgatcgaag tagccgccct tccagcccca
      841 gtaggcccag gtgccggcga ggcgatcgaa gacctgcttt gcgctccgtt ccgagccgaa
      901 tcgctcgtcg gcggggagcg ccgcaagcgc cgcctggtcg gcggtgcgcc gccacagcca
      961 gtccggcacg tcggcctcga ccacgggccc gagcttcgcc ggcacgcccg ccttgcggaa
     1021 atatttctgc gcgagaatgt cgcaggcgac ctggctccag gctgccggca cctcgatgtc
     1081 cggcagatgg aagacggtcg agccgtcggg attgcgaatc tcgctggtcg cccggcgaaa
     1141 ttcgatgctg ccgtaaggat cctgctccgc taccgtaaat cgccgctcga cacgcatccc
     1201 catcccccaa cgctaggcgt ttcttgtcct cgttgctttc cccgccggcg gggctctata
     1261 tgtagcgggc ggcccctcac cggaaactag atattggatg aagccgtgcc ggagagcaat
     1321 agattttgtg ggtggtttct cagaggcccc attagtagcg gcgccgagga tgttgcgcgc
     1381 cggcttttgc accccccagg cggaggtcca tggcgcgcgc gagaaagtgg gccacccctg
     1441 gacgcaagcg gcgtgcgatg gcatagtccc gccgctttcg cgtctagata tgacgcttgc
     1501 gttctcgcct gagagtggcg tttttcgcgg atgaatctcg gcacatcgct ctacacatgg
     1561 ctccacggcg agctggtcgg caccgatcag ttcggcaacc gctactatcg cgagaagcgg
     1621 cggcgggcgc tcaagcgcgg cggcgggatc gagagccgcg agcggcgctg ggtgctctat
     1681 aacggcgagg tcgaggcgtc gcgcgtgccg ccgctctggc atggctggct gcatcacatc
     1741 ttcgacgatg tgccggacga agccgcgcga aagaaatacc cgtgggagaa ggagtacgtg
     1801 cccaacctca cgggcaccga ccgcgcttat cgtccgccgg gctccctgct gcgcggcggc
     1861 catcgccagc gcgctgccgg tgactacgag ccctggaaac ccgagtgaac cggcgataat
     1921 cctagatcaa gggagcctcg aagcatgaac caaaacgccg tcgagaccgt gatgggcgcg
     1981 gtcgtgctgg tggtcgccgc cgtcttcctg ttcttcgcct attcgacgag ccggccgccg
     2041 agcctcagcg gctatgacgt gaccgcgaaa ttcgatcgcg tcgacggctt gcgcgacggc
     2101 ggcgatgtgc gcatcagcgg catcaaggtc ggtaccatcg tgtcgcagac gctggatccc
     2161 cagacctatc tcgccgtcgt gcacatgacg atcgacaatt cgatcaagct gccggtcgac
     2221 accattgcca ccatcaccgc gagcgggctc ctgggcgaca actatctggc gctcgtgccc
     2281 ggcggcgacg aggactacat caagccgaac ggccagatca cccacaccga ggccgccatc
     2341 aatctcttcg ggctcatcaa caaatacatc ggcagcggcg ccaacggcaa tggggcagcg
     2401 cccgggccgg cccccccacc ggcacagaac aagggtggtc agaaagctcc atccaagtag
     2461 cgtggcaccg cgaccgactt gtcgccgtcg gcctcgcacc tgacgccgtc ttctgggcgg
     2521 cgatgacggt gcgcaagacg cgcgcgagca gcgcaaagga gtgcatatgg tgaaccgcaa
     2581 tgccttcgag accgccatgg gcggggtcgt gttgttggga gcagcggtct tcatgctttt
     2641 cgtttacacg tccaccagat cgccggcgat gagcggcggc tacaacgtcg tagcccgctt
     2701 cacacgggtc gacgggctgc gggacggcag cgatgtgcgc gtcggcggta taaaggtcgg
     2761 cacggtgaca tcgcagcggc tcgacccgcg aacgcgcatt cccgaggtgc gaatgacaat
     2821 cgacaccgca cttcaaatcc caacggatac gatggcaacc atcgcgtcga cgggtttgct
     2881 gggcgagaac tacgtcgatc ttgaactcgg caatgatgag gaacaaatca aggcagacgg
     2941 cgtcattgct cgcaccgagg ccgctatcaa tatcttctca ataatcagca agtatctatc
     3001 ttgtgcatcg cggaacccga catgaccagc accagcccgc accagaagct cgtgctcccc
     3061 cgttggctca cgccccgggg ggagcccgtc tcctgtatcg acaagatcaa ggtgctgaac
     3121 aagaaccttg aggagctgcg cgacatggca caggaagcgt tcgaggatgc ggtgctgatg
     3181 ggttgcgacg aggcgcagtt ccgtggcgtt ctccatcagc tcgccgatgg cctcgtcaat
     3241 ccctatggaa aggaccggcc atgaagcggc attttctggg gttggcggcg gcgctcatcg
     3301 tcgctgcgac gggcgcacgg gcggaaacgg tcgcagtgct gcaaggcctc gacaagacga
     3361 cggcccgcat ctcgaaaatc gaggcgccgg tcggcaagag cgtgcgcctc ggcacgcttg
     3421 tgatcacggt gcgcgagtgc gtcaagcatc cgcccgaaga ggagccggag agcgcggcct
     3481 tcctgcagat cgacgagatg cgccagggcg acccgaccac cgccgtctcc gagcgcgtct
     3541 tcagcggctg gatgttcgct tcgagcccgg ccttgtccgc gctcgagaat ccgatctacg
     3601 acatcggcgt gctcgactgc aagagcgacg cgaccgcggc gccggcaagc ggctcgcgcg
     3661 ggaaataggc cttcaccgtc agcgcctgcg cgagacggcg gtggtattgc ccgcgcgcga
     3721 tctcgatggc gccgaattgc tggaggtgct cggtgacgaa ttgcgtgtcg agcaggcgga
     3781 agccgccgag cctgagccgc gccaccaaat gcgcgagcgc gatcttgctg gcatcgctga
     3841 cgcggctgaa catgctctcg ccgaaaaagg cgccgcccag cgccacgccg tagagcccgc
     3901 ccaccagctc gccctcgcgc caggcctcga cgctgtgcgc gaagccggtg cggaagagcg
     3961 cagcatagag ctgcacgatc tcctcgttga tccaggtctt gggccgctcg gccctgggct
     4021 cggcgcagcc gcgcaccacc gcttcgaagg cgcagttgca acggagctcg aaggcgtcct
     4081 ggcgtaaccg ccggcgcagc cggcgcggca gatggaaacc ctcgatcggc aaaatgccgc
     4141 gctgctccgg atcgacccag aagacatcgg gatcgtcggc cgactcggcc atcgggaaga
     4201 tgcctgcggc ataggcgcgc aacagaagat cgggcgtcag caccgtcatc gggcgcgagt
     4261 gtacgaaagc cgggagatgg agcaagccgg cacgttgcgc cgacgtgaag atccaccaca
     4321 aagacacaga agtgcagggg tttgcgagga aacggcgcag agcgcggtat cggtcagccg
     4381 atcgccgcga cgaagccttc gagccagtgg atatcgtagg cgccgttgat gaaatcaggc
     4441 gcggcgacga gccgctgatg gagcgggatg gtggtgtcga tcccggcgat cacgtattct
     4501 tggagcgcgc ggcgcatgcg catcaggcac tcattgcgcg tggtgccgta gacgacgagc
     4561 ttggcgacga gactgtcgta attcggcggc acggtgtagc cctgataaag cgcgctgtcg
     4621 acgcgcaccc cgaggccgcc cggcgcgaga tagctggtga cgcggccggg ggacggcatg
     4681 aaggtctggg aattctcggc gttgatgcgg cattcgatgg cgtggccccg cagctcgatg
     4741 tcctcctgcc gatagcccag cgtggcgccg gcggcgatgc ggatctgctc gcgcacgagg
     4801 tcgatgccgc tcaccatctc cgagatcgga tgctcgacct ggatgcgcgt gttcatctcg
     4861 atgaagaaga attcgccgtc ctgatagagg aactcgagcg tgccggcgcc ggcatagccg
     4921 atctccgcca tcgccgcgac ggcggtattg ccaatttggg cgcgggcgtc cgcgt
//
LOCUS       DASYNS010000412         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3069034, whole genome shotgun sequence.
ACCESSION   DASYNS010000412 DASYNS010000000
VERSION     DASYNS010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3069034"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="VGP50_12765"
     CDS             <1..925
                     /locus_tag="VGP50_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028297.1"
                     /translation="PVMLIFWPWAQQSPVLNPLRAIAYFSHEIFPFRTLFAGQYVLAS
                     DLPWAYLPVHIALALPELLLVLLLAAPVTAVVIARRRGWRLERDRALGLFIVGFGIVF
                     PVAYAIAIKAVLFDGMRHFIFVLPLIAALTAVLADRALDVMARLPQRRYAYGLLGIYA
                     LSHVSVMARLHPDEYVYYSGFIGGVEGAAGLFKLDYWANSYAEAVTGLEDHLRAEYGA
                     DFMNHDFTVADCGPPLSAAYYFPSNFIFTSNAREAEFFIAFTKDRCNKTLPGKEIFRV
                     ERMGTLLSVVIDRREVLAREGRYRRVARGGP"
     gene            948..1679
                     /locus_tag="VGP50_12770"
     CDS             948..1679
                     /locus_tag="VGP50_12770"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV8028298.1"
                     /translation="MERREYEKLAAAEEGTWWFGGLHAVLIDALAGATLAPLSTVLDA
                     GCGTGGLLARLKQTLPRAHLVGLDLDVVAAVLARAKSGCPVCIGSIDRLPLGEGTLDA
                     VLSADVLCHRGVDAAAGLTEIRRCLRPGGILVLNLPAYAWLWSAHDAAVDNERRFGRA
                     ELEHLLAAVGFAAVRVFYWNSFLFPLMVLRRKVWRHAGSDVGLLSAPVERLFALIMAL
                     ESRLRRAGLALPFGGSILALAVKPS"
     gene            1676..>1832
                     /locus_tag="VGP50_12775"
     CDS             1676..>1832
                     /locus_tag="VGP50_12775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV8028299.1"
                     /translation="MTPSLALSIVIPVYNGAQSIGEVVAALAGLSVPGGHEIVLVNDC
                     SPDNSLGV"
BASE COUNT          236 a          653 c          588 g          355 t
ORIGIN      
        1 gccggtaatg ctgatcttct ggccctgggc acagcagtcg ccggtcctca acccgctgcg
       61 cgccatcgcc tatttctcgc acgagatctt cccgttccgc acgctcttcg ccgggcaata
      121 tgtcctggcg agcgacctgc cctgggctta tctgccggtt cacatcgcgc tcgctttgcc
      181 cgagctgctg ctcgtactgc tcttggccgc gccggtcacg gccgtggtga tagcgcggcg
      241 ccgaggctgg cgcctggagc gcgaccgcgc gctcgggctc ttcatcgtcg gtttcggcat
      301 cgtctttccc gtcgcctacg ccatcgccat caaggccgtg ctcttcgacg gcatgcgcca
      361 tttcatcttc gttctgccgc tcatcgccgc gctcaccgcc gttctggccg atcgcgcgct
      421 cgacgtcatg gcgcggctgc cgcagcggcg ctatgcctat ggcctgctcg gcatctacgc
      481 cttgagccac gtctcggtga tggcgcggct ccatcccgac gaatatgtct attacagcgg
      541 cttcatcggc ggcgtcgaag gcgccgccgg tctcttcaag ctcgactact gggccaattc
      601 ctatgccgag gccgtcaccg gcctcgagga tcatctccgc gccgaatacg gcgccgattt
      661 catgaaccac gacttcacgg tcgcggattg cgggccgccc ttgtccgcgg cctattactt
      721 cccgagcaac ttcatcttca ccagcaatgc gcgcgaggcc gagttcttca tcgccttcac
      781 caaggatcgt tgcaacaaga ccctgccggg caaggagatc tttcgcgtcg agcggatggg
      841 cacgctgctc tccgtggtga tcgaccggcg cgaagtcctg gcgcgcgaag ggcgctatcg
      901 ccgcgtggcc cgcggcggac cttgacgcag cccggacagg ggacgccgtg gagcgccgcg
      961 aatacgagaa gctggcggcc gccgaggagg gcacgtggtg gttcggcggt cttcatgccg
     1021 tgctcatcga cgcgctggcg ggcgcgacgc tcgcacccct gagcaccgtt ctcgacgccg
     1081 gctgcggcac cggcggattg ctcgcccgtc tcaagcagac gttgccgcgc gcccacctcg
     1141 tcggactcga tctcgacgtc gtggcggcgg tgctcgcgcg cgccaagagc ggctgtccgg
     1201 tctgtatcgg ctcgatcgac cggctgccgc tgggcgaggg cacgctcgac gccgtgctca
     1261 gcgccgacgt gctctgccat cgcggcgtgg acgcggcggc gggattgacc gagatccggc
     1321 gctgcctgcg gccgggcggc atcctcgtgc tcaacctgcc ggcctatgcc tggctctggt
     1381 ccgcccatga cgccgccgtc gacaatgagc gccgcttcgg ccgcgccgag ctcgagcacc
     1441 tcctcgccgc cgtcggcttc gccgcggtcc gggtctttta ctggaacagt tttctctttc
     1501 ccctgatggt gttacggcgc aaggtatggc gccacgctgg gagcgacgtg gggttgctct
     1561 cggcgccggt cgagcgcctc ttcgctctca tcatggcgct cgagagccgg ttgcggcggg
     1621 cgggattggc cttgcccttc ggcggctcca tcctggccct cgcggtgaaa ccctcatgac
     1681 accttcgctg gcgctcagca tcgttatccc ggtctataac ggcgcgcaaa gcatcggcga
     1741 ggtggtggcg gcgctggccg gcctcagcgt gcccggcggc cacgagatcg tgctcgtcaa
     1801 cgactgcagc cccgacaaca gcctcggcgt gt
//
LOCUS       DASYNS010000413         1870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3694354, whole genome shotgun sequence.
ACCESSION   DASYNS010000413 DASYNS010000000
VERSION     DASYNS010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3694354"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VGP50_12780"
     CDS             complement(<1..197)
                     /locus_tag="VGP50_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV8028300.1"
                     /translation="MTNRKTLHPATLAAQALGSTDASTGAVVLPIHTATTFLRDPDNQ
                     YRRGRGYGRADNPSYDQPQALL"
     gene            complement(194..>1870)
                     /locus_tag="VGP50_12785"
     CDS             complement(194..>1870)
                     /locus_tag="VGP50_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV8028301.1"
                     /translation="AGGRRRLAPSNWESLVVTWEQLAFWASSLIFLFAAMRTPELLEK
                     ASWADLGLLAALVAAALAARALTLYGLIPMLSFAGIAKPIDRDHKLVMLWGGMRGAVS
                     LALALAAAENTALPPEVRQFVGILATGFVLFTLLVTAPTMRPLMRLLKMSSLAPAEVA
                     VSQRVIGLSLSSIPQEIETVGRQHQIAPEIVAEVAKAYDGHGREPAGAAVENAELPAE
                     LRERAALYVLADREQEFCLDYFENRTVSRRVAASLLAHAARLRDGARTDGVIGYQKAA
                     ARILAFNRALRLSRAVHHRIGIARPLARLLGDRFEVQLAARFVLQNVIAFNRDQIEEV
                     FGAGPRAAMAEQLDQRFGAIDRAIAALKLQYPTYARQLEVQYLARLAARLEEERYRRL
                     RAESIINQDMYEDLQRKLRPRRRAVEAHPTLDLGLKREDLVGRVAMFAALDAKTRRSV
                     ARMLRPRLAVPDEVIVHKGERGDAMYFISSGAVEVRIAPASVHLGSGDFFGELALLVA
                     DRRTADVVALGYCQLLSLAARDLHRLFGTEPALRDQIHAVAQARTATAET"
BASE COUNT          341 a          614 c          616 g          299 t
ORIGIN      
        1 agcaacgcct gcggctggtc gtagctcggg ttgtcggcgc gtccgtaacc gcggcctcgc
       61 cggtattggt tgtcgggatc gcgcaagaac gtcgtcgccg tgtggatcgg caagacgaca
      121 gcaccggtcg acgcgtcggt cgagcccagc gcctgcgcgg caagggtggc gggatgcagc
      181 gttttacggt tagtcatgtt tccgctgttg cggtgcgcgc ctgggcgacg gcgtggatct
      241 ggtcgcgcaa ggccggctcg gtcccgaaga gacgatgaag atctcgcgcc gccaggctca
      301 acagctggca atatccgagg gccacgacat cggcggtgcg gcggtcggcg accagcaagg
      361 cgagctcgcc aaaaaaatcg ccgctgccga ggtggacgga ggcgggtgcg atccgcactt
      421 ctaccgcgcc cgaagagatg aaatacatcg cgtcgccacg ttcccccttg tggacgatga
      481 cttcgtccgg caccgcaagc cgcggccgca gcatgcgggc gacagagcgc cgggtcttcg
      541 catcgagcgc cgcgaacatc gccacccggc cgaccagatc ctcgcgcttc aggccgagat
      601 cgagggtcgg atgcgcctcg acggcacggc gccgcggccg caacttgcgt tgcaagtcct
      661 cgtacatgtc ttggttgata atcgattcgg cgcgcaatct gcggtaccgc tcctcctcca
      721 ggcgcgccgc cagacgggcg agatactgca cttcgagctg acgcgcataa gtcgggtatt
      781 gcagcttcag cgctgcgatc gcgcgatcga tcgcgccgaa tcgttggtcg agctgttccg
      841 ccattgccgc tctcggcccg gcgccgaaca cctcctcgat ctgatcgcga ttgaacgcga
      901 tcacgttctg caagacgaag cgagccgcca gctggacctc gaaccggtcc ccgagcagcc
      961 gcgcgagagg gcgcgcgatg ccgatccgat gatggaccgc gcgcgacaac cgcaaggccc
     1021 tgttgaaggc aagaatccgg gccgccgcct tctggtaacc gatgacaccg tcggtgcgcg
     1081 cgccgtctcg gagcctcgct gcatgagcga gaaggctggc cgccacccgc ctcgacactg
     1141 tccggttctc gaaatagtcg agacagaatt cctgctcgcg atcggccaga acgtaaagcg
     1201 ctgcccgctc cctcagctcg gcgggaagtt cggcattctc gacggcagct cccgcgggct
     1261 cgcggccatg gccgtcatat gcctttgcga cctcggcgac gatctcgggc gcgatctggt
     1321 gctgccgtcc gacggtctcg atttcctgtg ggatagacga aagggaaagt ccgatcaccc
     1381 gctggctgac tgcaacttcg gccggagcga ggctgctcat cttgaggagg cgcatcaagg
     1441 gccgcatggt cggcgccgtt accagcagcg tgaacaacac aaaaccggtc gcgagaatgc
     1501 cgacgaactg acgaacctca ggagggagcg cagtgttctc ggcggcggcg agcgccagtg
     1561 cgagagaaac cgctccgcgc atgcctcccc ataacatgac gagcttgtgg tcgcggtcga
     1621 tcggctttgc gataccggcg aaactgagca tcggaatcag gccgtagagc gtcagcgccc
     1681 gcgcggcgag agccgcggcg accagcgcgg ccagcaagcc cagatcggcc cagctcgcct
     1741 tttcgagaag ttcgggcgtg cgcatcgccg caaacaagaa gatcagcgag ctcgcccaga
     1801 aggccagctg ctcccatgtg acgacgaggc tttcccaatt cgacggcgct aagcggcgcc
     1861 ggccgccagc
//
LOCUS       DASYNS010000414         2184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1196665, whole genome shotgun sequence.
ACCESSION   DASYNS010000414 DASYNS010000000
VERSION     DASYNS010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196665"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VGP50_12790"
     CDS             <1..1198
                     /locus_tag="VGP50_12790"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEV8028302.1"
                     /translation="VELVIVPPDWAAAAYHAVVPTLSGMDALTLAVAIVGATVMPHAI
                     HVHSGLTHGRIVPRSEGERRKLIRYSNREVLLALGLAGLVNMAMVAMAASVFHHPGLD
                     AVTGLETAYRTLVPLLGFGAAGVFLVSLIASGLSSSAVGTMAGQIIMQDFVGFRIPIW
                     VRRLVTMAPAFVVVALGVDTWRALILSQVVLSLVLPVPMLALLTLTRQRSIMGSAVNG
                     RLTAALGTLAASVVLALNGLLILHLFGMSPIELAQILERRLDLAAALAMAFIFVLLTP
                     LWSSLAFPQLALAKNASMVLTVIERLPNGEALARDSAQSWQPRMILPVGIDAPEDPAT
                     GSLTVPLPADWLDSVGLQVNPPPGASGARVFHMVGTVDGRQVRDIAIMVNAGAGETPL
                     PTPATP"
     gene            1271..2179
                     /locus_tag="VGP50_12795"
     CDS             1271..2179
                     /locus_tag="VGP50_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028303.1"
                     /translation="MTNNAQDAASVELTAVVVAVTGEEPRVLTIQEGRGLPSGPLESA
                     HRTLQMGLRSWVERQTHHPLGYMEQLYTFADSDRMRSGGRVISISYLGLTREAHAPEG
                     QEGSWQDWYRYLPWEDWRAGRPPWLTETVRSLRQWARTASEPATRRDRVRRIDVAFGE
                     DESWNEELVLQRYELLYEGGLVAEAGAPSSLPGQAMILDHRRILATGIARLRAKIKYR
                     PVVFELMPDSFTLLQLQRAVEALAGRRLHKQNFRRLIDQQGLVEETGAISTGTGGRPA
                     KLFRFRREVLRERAVSGSKLPLSRSA"
BASE COUNT          299 a          769 c          745 g          371 t
ORIGIN      
        1 cgtcgagctc gtcatcgtgc cgcccgactg ggccgcggcg gcgtatcacg ccgtggtgcc
       61 gacgctctcg ggcatggacg cgctgaccct cgccgtcgcc atcgtcggcg cgacggtgat
      121 gccgcatgcc attcacgtcc attcgggact gacccatggc cgcatcgtgc cgcgcagcga
      181 gggcgagcgg cgcaagctca ttcgctattc gaaccgcgaa gtgctgctgg cccttggtct
      241 tgcgggtctc gtcaacatgg caatggtggc catggcggca tcggtgttcc accaccccgg
      301 cctcgacgcc gtcaccgggc tcgagaccgc gtatcgcacg ctcgtgccgc tgctcggctt
      361 cggcgccgcc ggcgtgttcc tggtgtcgct gatcgcgtcg ggcctctcga gctcggcggt
      421 ggggaccatg gccggccaga tcatcatgca ggacttcgtc ggtttccgca ttccgatatg
      481 ggtccgccgc ctcgtgacca tggcgcccgc tttcgtggtg gtggcgctcg gcgtcgacac
      541 ctggcgggca ctgatcctga gccaggtcgt gctgagcctg gttttgccgg tgccgatgct
      601 ggcgcttctc acgctgacgc ggcagcggag catcatgggc tcggccgtca atggacgcct
      661 gaccgccgcc ctcggcacgc tcgccgcgag cgtcgtcctc gcgctcaacg gcctgctcat
      721 tctccacctc ttcggcatgt cgccgatcga gctggcgcag atcctggaac ggcgcctcga
      781 cctggccgcg gcgctggcca tggcgttcat tttcgtgctg ctgaccccgc tctggtcgag
      841 cctcgccttt ccccagctcg ccttggccaa gaacgcgtcc atggtgctga cggtgatcga
      901 gcgactgccg aacggcgagg cgctcgcgcg cgacagcgcc caatcatggc agccgaggat
      961 gatcctgccg gtcggcatcg atgcgccgga ggatcccgcg acgggcagcc tgacggtgcc
     1021 gctgccggcc gattggctcg attcggtcgg gctgcaggtg aacccgccgc cgggcgcgtc
     1081 gggagcacgc gtctttcata tggtcgggac ggtcgatgga cggcaggtcc gcgacatcgc
     1141 catcatggtc aatgccggag ccggcgagac cccgcttccc acgccggcga ctccctgacg
     1201 cacccgcccc gggcttcgcc gtttccgcgc cgagcggctg tggcataatg cctccgcttt
     1261 ggacatgccc ttgaccaaca acgcacagga cgccgcgtcc gtcgagctga cggcggtcgt
     1321 ggtcgccgtc accggcgagg agccgcgggt cctgacgatt caggagggcc gagggctccc
     1381 gtcgggcccg ctcgaatcgg cgcatcgcac cctgcagatg ggcctccgct cctgggtcga
     1441 gcggcagacc catcatccgc tgggatatat ggagcagctc tacaccttcg ccgatagcga
     1501 ccgcatgcgc agcggcgggc gcgtcatctc gatcagctat ctcggcctca cgcgcgaggc
     1561 gcatgcgccc gaggggcagg aagggagctg gcaggattgg tatcgctatc tcccctggga
     1621 agattggcgc gcggggcgcc cgccgtggct gacggagacc gtgcggtcct tgcggcaatg
     1681 ggcgcggacg gcgagcgagc ctgcgacgcg gcgcgaccgg gtgcggcgca tcgacgtcgc
     1741 cttcggcgag gacgagagct ggaacgagga gctggtgctg cagcgctacg agctgctcta
     1801 cgaaggcggc ctcgtcgccg aagccggcgc cccgtcgtcg ctgccggggc aagcgatgat
     1861 cctcgaccat cgccgcattc tcgccaccgg gatcgcgcgg ctcagggcca agatcaaata
     1921 ccgtcccgtc gtgttcgagc tgatgccgga cagcttcacg ctgctgcagc tccagcgcgc
     1981 ggtcgaggcg ctggccggac ggcgcctcca caagcagaat ttccgccgcc tcatcgatca
     2041 gcagggactg gtcgaagaga ccggcgccat ctccacgggc accggcggcc gtcccgccaa
     2101 gctcttccgc ttccgccgcg aagtgctgcg cgagcgcgcc gtttccggct cgaagctgcc
     2161 gctgtcgcgc tcggcctagg actg
//
LOCUS       DASYNS010000415         6600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4629941, whole genome shotgun sequence.
ACCESSION   DASYNS010000415 DASYNS010000000
VERSION     DASYNS010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4629941"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VGP50_12800"
     CDS             <1..678
                     /locus_tag="VGP50_12800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV8028304.1"
                     /translation="MKVLIADDHALVRQALRQLLDDEELGLAKNDHALSFIEADGFDA
                     ALEGLAANGADLMLIDLSMPGMAGAASLRALREAYPQTKIVVVTGWEDRATILECLSA
                     GVHGYVLKSFAPDQIVHAIEIILAGGVYVPPEIANLRTADGTRENGVKNGIPAAPAAP
                     ANTRFTKRQLDVLQLLGEGRSTKEIARALELSEGTVKVHLAAIYRCLNARNRTEAVAL
                     AGKLQRQ"
     gene            849..1349
                     /locus_tag="VGP50_12805"
     CDS             849..1349
                     /locus_tag="VGP50_12805"
                     /inference="COORDINATES: protein motif:HMM:NF025710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HEV8028305.1"
                     /translation="MPTVRIISTIALMGAATALLLPADALAATATGHASVTIVAAVAV
                     SEAAPMDFGTVGVTKAGGTIVLSPNGTRSGPATYSFHGSSRPGQFTVTGTPNAAVAIS
                     FSSGDAVTGPGPAIALGSFTHNAGPAPALNSAGTLSLAVGATMAVGAAQPYGSYAGSY
                     TVTVNY"
     gene            1381..1713
                     /locus_tag="VGP50_12810"
     CDS             1381..1713
                     /locus_tag="VGP50_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028306.1"
                     /translation="MSPQNFGGWSSLLQERAEHIRGVRSIAVATRIPVVVQGLDELAI
                     LYGDTASGGMPRRGDPKRDLIVRLQQKAGSFLQQASAVNDAARAEQLRSLSAIFGAEA
                     QRLQSDAA"
     gene            complement(1741..2367)
                     /locus_tag="VGP50_12815"
     CDS             complement(1741..2367)
                     /locus_tag="VGP50_12815"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEV8028307.1"
                     /translation="MQESREREDSPLHVPEIVECLARRSELWIFGYGSLMWDPCFPYS
                     EAQPALLRGYHRSFCVYSRRYRGTPERPGLVLGLDRGGACKGIAFRVPAAEVEAALDR
                     LWRRELTQRTYRLKELRLETAQGALAARALIVDRGHPNYAGRLPLAGVARHILQGMGT
                     RGSCRQYLENTVCELRKLGRVDGPLHRLAEMVTDLAAQERRFSLDKPA"
     gene            2530..3534
                     /locus_tag="VGP50_12820"
     CDS             2530..3534
                     /locus_tag="VGP50_12820"
                     /inference="COORDINATES: protein motif:HMM:NF021423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2125 domain-containing protein"
                     /protein_id="HEV8028308.1"
                     /translation="MRRSILLSGTAIALAAAAYGGYWFTTAERLRSGIERWAAARQAE
                     GRDLHWTSVTSEGFPFAFRLRITGALLDGSRPLPFSAATPILLGEAQPWNLRRWTLSA
                     PSGAQLAMPIEATTLVAAALDGTLLLPASGGMAVTLRAQDVAASGNTQLRIGEAELKL
                     ALPDHAPASHHDTALDAALRLARLTLPHPLPPLGDTVDSLSVAGTLKGTLPQGPLRQS
                     LALWREDGGTIELEDGSIQWGALALSATGTLSLDEGLQPIGALTATVVDQNAVIDAAV
                     AQGSMRAGDANLAKVVLGMMAKPGADGKSRLTVPLSIQNHHLFLGPAQIATLPRFSWE
                     "
     gene            complement(3580..3786)
                     /locus_tag="VGP50_12825"
     CDS             complement(3580..3786)
                     /locus_tag="VGP50_12825"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEV8028309.1"
                     /translation="MDRVVMAVDSPLAEIGARVVVVVALLSCWRDRAFQRRQLARLDA
                     RQRADIGITQCDVAQECAKPFWRA"
     gene            complement(3882..4766)
                     /locus_tag="VGP50_12830"
     CDS             complement(3882..4766)
                     /locus_tag="VGP50_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HEV8028310.1"
                     /translation="MSANGFERVALIGIGHIGSSLARVLRRDKLAREIVACDRRAETL
                     DKVRSLGLAEVVTDDPAEAARGADLVVIATPLSAYAEIGRSIAPVLMSGAILTDVGSV
                     KGAVVRDLQPLLPEGVHLVPGHPVAGTEHSGPESGFAEVFQNRWCILTPLPGGDPAAL
                     ARVRALWESAGMKIVLMDAAHHDMVLAITSHLPHLIAYTIVGTATDLEESLKSEVIKF
                     SAGGFRDFTRIAASDPVMWRDVFLNNREAVLEMLQRFNEDLTALQRAIRWGEGEKLEE
                     LFTRTRAIRRSVIEAKQA"
     gene            complement(4763..5857)
                     /gene="hisC"
                     /locus_tag="VGP50_12835"
     CDS             complement(4763..5857)
                     /gene="hisC"
                     /locus_tag="VGP50_12835"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEV8028311.1"
                     /translation="MAANPTPRPGILDIAPYVGGESKVTGISRVIRLASNESALGPSR
                     RAVEAYHAAASELHRYPDGASHELREALGRRHGLDPRRIVCGTGSDELINLLARCYAG
                     PGDEVLYSRHGFLLYPIAAQAVGALPVAAPEKSLTADVDGLLARVSEKTRLVFIANPN
                     NPTGTFVPRDAIRRLHAGLPRSTVLVIDSAYAEFVTRNDYEPGIELVDAAENVVMLRT
                     FSKIYALGGLRIGWGYFSAPIADVMNRVRGVFNINLPAQAAAVAALEDIAAVDRAREH
                     NDIWRPWLERELEALGLTVTPSVANFVLVRFAAAPRNAEAAFAFLQSRGILVRKTRAY
                     GLGEHLRITIGLEEELRAVVAALAEFLSAP"
     gene            complement(5863..>6600)
                     /locus_tag="VGP50_12840"
     CDS             complement(5863..>6600)
                     /locus_tag="VGP50_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV8028312.1"
                     /translation="TIRPGREALILRRLVERHRGRFPRAVLVRLWRELLSGTIAMQGS
                     FAVAVYMPRSTPDYWDLARDHYGSHTPMMAFHSASELLRTLGEGRAAVGVLPMPAEGE
                     SAPWWPLLTASGAAVPQIIARLPFAGSGNARVDGGEAVVIGHAELDPTGADRSFIALE
                     IGGELSRARLIGALNDAGLMVTLFAAHEPHGSSVLHLVEIDDLVSPGDPRLVRALQPL
                     GDRVTRVAHLGAYARPVAAAVLDGRQA"
BASE COUNT         1103 a         2303 c         2204 g          990 t
ORIGIN      
        1 atgaaagtct tgattgccga tgatcatgcc ttggtccgcc aggccctgcg ccagctcctg
       61 gacgatgagg agctgggcct cgccaagaat gaccacgcgc tgagctttat cgaggccgac
      121 ggcttcgacg cggcgctcga agggctcgcc gccaacggcg cggatctgat gctgatcgac
      181 ctgtcgatgc ccggcatggc cggcgccgcc agtctccggg cactgcgcga ggcttatcct
      241 cagaccaaga tcgtcgtcgt caccggctgg gaggatcgcg ccaccattct tgaatgcctc
      301 tccgccggcg ttcatggcta tgtgctgaaa tccttcgctc ccgaccagat cgttcacgcc
      361 atcgaaatca tcctcgccgg cggcgtctat gtgccgccgg agatcgccaa tctgcgcacg
      421 gccgacggaa cgcgcgagaa cggtgtgaag aatggcattc cggcggcgcc cgccgcgccg
      481 gccaacaccc gcttcacgaa gcgccagctc gacgtgcttc aactcctcgg cgagggccgc
      541 tcgaccaagg agatcgcccg cgcgctcgag ctcagtgaag gaaccgtaaa ggtccatctc
      601 gccgcgatct atcgctgcct caatgcacgc aaccgcaccg aagcggttgc gcttgccggc
      661 aagcttcagc gccaatagcg gcgcctcgcc gcgcttgccc atcggctgat gccctcggcc
      721 taagcctcca ccggacaagc agccgacacc ccgaccgacg cttcccgcct tagccgccgc
      781 gccgactatc tcgcgagacg aaaacccgtc aaattgttta cataaagcgg gagcgcggat
      841 tgaagccaat gccgacggtc agaatcatca gcaccatcgc cctcatgggg gcagccacgg
      901 cgctcctcct tccggcggac gcgctggcgg cgacggcgac gggtcatgcg tccgtgacga
      961 tcgtcgccgc ggtcgcggtg agcgaggccg cccccatgga tttcggcact gtcggcgtga
     1021 ccaaggccgg cggcaccatc gtgctcagcc ccaacggcac ccgcagtggg cccgcaacct
     1081 attccttcca cggctcgtcg cggcccggcc aattcaccgt gacgggcacg cccaacgcgg
     1141 ccgtcgccat ctccttcagc tcgggcgatg ctgtgaccgg gcccggcccc gccatagcgc
     1201 ttggctcctt cacccacaat gccggcccgg ctcccgcgct caacagcgcc ggcactctga
     1261 gcctggccgt cggcgccacc atggcggtcg gcgcagcgca gccttacggc agctatgccg
     1321 ggagctacac cgtcaccgtc aactactgaa cgcgaccgtt gtcgctcatt ggaggctcgg
     1381 atgtcgccgc agaactttgg aggctggtca tcgttgctgc aggagcgggc ggagcatatc
     1441 cgcggcgtgc ggtcgatcgc cgtcgccacg cgcattccgg tcgtggtgca gggcctcgac
     1501 gagctggcga ttctctatgg cgacaccgcc agcggcggga tgccgcggcg gggcgatccc
     1561 aagcgcgacc tgatcgtccg gctgcagcag aaagccggca gcttcctcca gcaggccagc
     1621 gccgtgaacg acgcagccag ggcggagcag ctgcgctcgc tctcggcgat cttcggcgcc
     1681 gaagcccaac ggctgcagtc ggacgccgcc tgacacatcc cgcgcgcagg cgcgagcgcg
     1741 ctatgcgggc ttgtcgaggc tgaaccgacg ctcttgcgcc gccagatctg tcaccatctc
     1801 cgcgaggcga tgcaggggcc cgtcgacgcg gccgagcttg cgcagctcgc acaccgtatt
     1861 ctccagatac tggcggcatg agccgcgcgt gcccatgcct tgcaggatat gccgcgccac
     1921 accggcgagc gggagccggc cggcatagtt gggatggccg cgatcgacga tgagtgcccg
     1981 cgccgccaac gcaccctgcg ccgtctcgag ccgcagttcc ttcagccgat aggtgcgctg
     2041 cgtcagttcc cgccgccaca ggcgatcgag cgcggcctcg acctcggccg ccggcacgcg
     2101 gaacgcgatg cccttgcagg cgccgccgcg atcgaggccg agcaccagtc ccggacgctc
     2161 gggggtgccg cgatagcgcc gcgaatagac gcagaagctg cggtgatatc cccggagcag
     2221 cgccggctgc gcctcgctat aggggaaaca cgggtcccac atcagcgacc catagccgaa
     2281 gatccaaagt tccgagcgcc gcgccaggca ctcgacgatc tcgggcacgt gcaggggcga
     2341 gtcttcccgc tcgcgcgatt cttgcatatg ggtgccgcct cgtcctccgc cgaaacctgg
     2401 tcgccgatgc acagaccggg ccaccatggc gaatattttg ccagggcccg cgcgcgcgca
     2461 agcgccggac gcgcatggcc ggcccgcgtt tcgcatcgac ccgcggccgt cccggcccta
     2521 taaggccgca tgcgtcggag catccttctc tcgggcacgg cgatcgctct cgctgcggcg
     2581 gcctatggcg gctactggtt cacgaccgcc gagcggctgc gctccggtat cgagcgctgg
     2641 gctgccgcgc gccaggcgga ggggcgtgac ctgcactgga cgtcagtgac cagcgaaggg
     2701 tttcccttcg ccttccgcct gcgcatcacc ggcgcactcc tcgacggctc gcggccgctg
     2761 cccttttccg cggccacccc gatcctgctg ggcgaggcgc agccctggaa tctgcgacgc
     2821 tggactctga gcgccccgag cggggcgcag ctcgcgatgc cgatcgaggc aacgacgctc
     2881 gtcgccgcag cgcttgacgg cactttgttg ctgcccgcgt caggcggaat ggcggtcacg
     2941 ctcagagcgc aggacgtggc ggcgagcggc aacacccaac tgcgcatcgg cgaagccgag
     3001 ctcaagctcg cactgcctga ccatgcaccg gccagtcatc atgacacggc gctcgacgca
     3061 gcattgcgcc tcgccaggct gaccttgcct catcccctgc cgccgctcgg cgacacggtc
     3121 gatagcttga gcgtcgcagg cactctgaaa gggacgctgc cgcagggccc gttgcgccag
     3181 tctctggcgc tctggcgcga ggacggcgga acgatcgagc tcgaggacgg ctcgatccaa
     3241 tggggcgcgc tggcactctc ggcgactggc acgctgtcgc tcgacgaggg gctccagccg
     3301 atcggcgcct tgaccgcgac cgtcgtcgac cagaacgccg tcatcgacgc ggcggtagcg
     3361 cagggcagca tgcgcgcggg cgacgccaac cttgcgaagg tcgtcttggg gatgatggcg
     3421 aagcccggcg ccgacgggaa atcgcggctg accgtgccgc tcagcatcca gaaccaccac
     3481 ctttttctcg gcccggcaca gatcgcgact ctgccccgct tctcgtggga gtagcgcccg
     3541 ccgcgccgtc gggttgcgca tcagcttcgc accggcggct caggcgcgcc agaacggctt
     3601 cgcgcattcc tgcgcgacgt cgcactgcgt aatgccgata tccgcgcgct ggcgcgcatc
     3661 cagccgcgcg agctgccgcc gctggaacgc gcgatcgcgc cagcacgaca gcagcgcgac
     3721 gacgacgacg accctcgcgc cgatctcagc caacgggctg tcgaccgcca tcacgactcg
     3781 atccatgatc cacctcctca gtcgttgctg agggcaatat ggcgccgcgc acgtgctgcc
     3841 gtgcgcggaa acgcttcggc cccggccgaa gcgaagccgt gtcaggcctg cttcgcctcg
     3901 atgaccgagc ggcggatggc gcgggtgcgg gtgaagagct cctcgagctt ctcgccctcg
     3961 ccccagcgga tggcgcgttg cagtgccgtc agatcctcgt tgaaacgctg cagcatctcg
     4021 agcacggctt cgcggttgtt gaggaacacg tcacgccaca tcacgggatc ggatgcggca
     4081 atgcgcgtga agtcacggaa gccgccggcc gagaatttga tgacttcgga tttgaggctc
     4141 tcctcgagat ccgtcgcggt gccgacgatg gtgtaggcga tgagatgggg cagatgcgag
     4201 gtgatcgcca gcaccatgtc gtgatgcgcg gcgtccatga ggacgatttt cataccggcg
     4261 gattcccaga gtgcccgtac gcgcgccagc gccgcgggat cgccgccggg cagcggcgtc
     4321 aggatgcacc agcgattctg gaagacctcg gcaaaacccg attcggggcc ggaatgctcg
     4381 gtgccggcga cgggatggcc aggcacgaga tgcacgccct cgggcagcaa tggctgtaga
     4441 tcccgaacca cggcgccctt gaccgagccg acatcggtga ggatggcgcc ggacatcagc
     4501 accggcgcga tggagcggcc gatctcggca taggcggaca gcggcgtcgc gatgaccacg
     4561 agatcggcgc cacgggccgc ttcggccgga tcgtcggtga cgacctcggc aagccccagc
     4621 gatcgcactt tgtcgagcgt ttcggcgcgc cggtcgcaag cgacgatctc gcgcgccagc
     4681 ttgtcgcggc gcagcacgcg ggcgagcgaa gagccgatat ggccgatgcc aatcagcgcc
     4741 acgcgctcga agccgttcgc gctcacggcg cgctcaggaa ttcggcgagc gcggcgacca
     4801 cggcgcgcaa ttcctcctcg aggccgatgg tgatgcggag atgctcgccg agcccgtaag
     4861 cgcgcgtctt gcgcaccagg atgccgcgcg attgcagaaa ggcgaaggcg gcttcggcat
     4921 tccgcggcgc cgccgcgaag cgcacgagga caaagttcgc gacgctcggc gtcacggtga
     4981 gccccaaggc ttcgagctcg cgctcgagcc agggacgcca gatgtcgttg tgctcgcggg
     5041 cgcgatcgac cgccgcaatg tcttcgagcg ccgccaccgc cgccgcttga gccggcaggt
     5101 tgatgttgaa gacgccgcgg acgcggttca tcacgtccgc gatgggcgcg ctgaaatagc
     5161 cccagccgat gcgcaagccg cccagcgcat agatcttcga gaaagtgcgc agcatcacga
     5221 cattctctgc ggcatcgacg agctcgatcc cgggctcgta gtcgttgcgc gtgacgaatt
     5281 ccgcataggc cgagtcgatc accagcaccg tcgagcgcgg cagcccggca tggagacggc
     5341 ggatcgcgtc ccgcggcacg aaggtgccgg tgggattgtt cggattggcg atgaagacca
     5401 gacgcgtctt ctcgctgacc cgggcaagga ggccgtcgac gtcggcggtc agagattttt
     5461 ccggagcggc gacaggcaat gcgccgacgg cctgagcggc gatcggatac aaaagaaagc
     5521 cgtggcggct gtagagcact tcatcgcccg gtcccgcata gcagcgcgcc agaaggttga
     5581 tgagctcgtc cgatcccgtg ccgcagacga tacgacgggg atcgagacca tggcggcgtc
     5641 ccagcgcttc gcgcagctca tgggacgcgc cgtcgggata gcggtggagc tcgctcgccg
     5701 ccgcatggta ggcctcgacg gcgcggcggc tgggcccgag ggcgctctca ttggaagcga
     5761 ggcggatcac gcgagagatt cccgtaacct tcgactcgcc gccgacatag ggcgcgatgt
     5821 cgagaatgcc ggggcgcggc gtcgggttgg cggccatgat gctcacgcct ggcgcccgtc
     5881 gagcaccgct gcggcgacgg gacgcgcata ggcacccaga tgcgccacgc gcgtcacacg
     5941 atcacccagc ggctgcagtg ctcttactag tcgcggatcg cccggcgata cgaggtcgtc
     6001 gatctcgacc agatggagca cgctcgatcc gtgaggctca tgagcggcaa agagcgtgac
     6061 catgagcccg gcatcgttga gcgcgccgat gagccgcgcg cggctcagct caccgccgat
     6121 ctcgagcgcg atgaagctgc gatcggcgcc ggtcggatcg agctcggcgt ggccgatcac
     6181 caccgcctcg ccgccatcga cgcgcgcatt gccgctcccg gcgaaaggca atcgggcgat
     6241 gatctgcggc acggcggcgc cgcttgcggt caacagcggc caccacggcg cgctctcgcc
     6301 ttcagcgggc atcggcaaca cgcccacggc ggctctcccc tcgcccagcg tgcgcagcag
     6361 ctcgctggca gagtgaaagg ccatcatcgg cgtgtggctg ccatagtggt cgcgcgcgag
     6421 atcccaatag tccggcgtgc tccgcggcat gtacacggcg acggcgaagc tgccctgcat
     6481 cgcaatggtg ccgctcagga gctcgcgcca aagacgcacc aggacggcac gcggaaaacg
     6541 gccgcggtgg cgctcgacga gacggcgcag gatcagcgct tcgcgcccgg ggcggatggt
//
LOCUS       DASYNS010000416         8197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_106675, whole genome shotgun sequence.
ACCESSION   DASYNS010000416 DASYNS010000000
VERSION     DASYNS010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106675"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VGP50_12845"
     CDS             complement(<1..963)
                     /locus_tag="VGP50_12845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated RND transporter
                     HpnN"
                     /protein_id="HEV8028313.1"
                     /translation="MLALIAGLVETSRRHAAPLVAALLLLSVIGGYFVARNISLDTNI
                     DKLINPNLPWRQQERKLNQAFPQNADLLAVVIDARTVDEAQDASAALAARLAEQKTLF
                     KSVRQPDGGRFFEQNGLLFLSQADVQIFADQIVAAQPLIGTLAADPSLRGVFDALDLA
                     AQGAVRESDPPPQLEAAFNTAAAATEAALEGRYDPLSWASLLTGQKQEPEELRRIVLA
                     QPILDYRELQPGASATGAIHDAALALGLTPDRGIRVRVTGSVALSDAEFSTLSKGAAL
                     STTLSLSLLCLWLLLALRSLRLLVAIVTTLVVGLVGCGVFAVGVV"
     gene            complement(968..2620)
                     /locus_tag="VGP50_12850"
     CDS             complement(968..2620)
                     /locus_tag="VGP50_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011630752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEV8028314.1"
                     /translation="MALLLDIFGFLSVILRGVVLTAQSLTIGGIVFLSLLLDPLTPRL
                     GSDGAAARRRCHALLRWSAVVLAGVELVFVLTEASVLAGTIDISLADALGADFARLGI
                     VAMAASLAAAALTGRPIATGRFSPLPLLGITILVAQVASSHAAAQLNGRAPLLIADFA
                     HMAGAAAWIGGIPYLLLTLQQMRNPTDEARIGRRFSLIAMGAVAILVGAGIVMAFTYI
                     GSLEAFYGTAYGVMVGTKVLLLVGLLFLGAMNYRVVERLRRDPRTPILRLRRFAEVEI
                     GVGLAVLFAAASLTSQPPAVDLTTDRASLHDIVERLTPHWPRFESPDYASLALSELDA
                     QLRGTVRTDAPRPEAYVPGEGIAPPRNAEDIAWSEYNHHWAGLFVLAIGLLALLERTG
                     RAPWARHWPLVFLGLAAFLFLRSDPEVWPLGPVGFIASLRDPEVVQHRIFVLLTVAFA
                     LFEWRVRTGRLRRPGAALVFPLTTALGGTLLLTHSHALANLKDQLLIEISHVPLALCG
                     ITAGWSRWLELRLDGRGSRIAAWVWPVAFVLVGLILLSYREA"
     gene            complement(2628..2984)
                     /locus_tag="VGP50_12855"
     CDS             complement(2628..2984)
                     /locus_tag="VGP50_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEV8028315.1"
                     /translation="MRLAFAPGLAALLLAAPVSALAHAIIVAAEPAAGAVLHGSKAQV
                     LLRFNSRIDVERSRLTLISPDGETRPLALAPSDGPDTLAATMDGLGSGAYRLRWQVLA
                     VDGHITRGDIPFTVAP"
     gene            complement(3054..3731)
                     /locus_tag="VGP50_12860"
     CDS             complement(3054..3731)
                     /locus_tag="VGP50_12860"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEV8028316.1"
                     /translation="MRDKDDGLAALEPAPLQFHVHLLARHRAARGEGLIHRQQQRAPA
                     GVNWRQAAAMLPLAAILALSRPAQAEGWLDEGKLGLMYHDIPIGGDHREPGPDINGEL
                     LFASPDFLQAIGAPRPHLGGSLNTAGATSYGYFGLTWTLAPWDGPLFFGLGLGGAVHD
                     GDLDKDIPDRKELGSRILFHESVEGGWRISRRFSLSAFLDHMSNADLATHNAGMTNLG
                     LRTGYKF"
     gene            complement(3963..4976)
                     /locus_tag="VGP50_12865"
     CDS             complement(3963..4976)
                     /locus_tag="VGP50_12865"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV8028317.1"
                     /translation="MTKRLEATTSMTRTIEWQGEDVAAKATPRRFAMPVPSREEVRAG
                     ILYMMGAVLVFSAINAGVKWEAARYPLDEVVFLRCVFSLLPCMALIAAHGGFRLMRTR
                     RLREHIGRAVMQFISILSIFAAFAMMPLADAVAISFSSPLFLTLLSIPLLGERVGRHR
                     WAAVLIGFVGVLLMVRGGGGFAGGLASTGALLALMSAAIGASVTIAVRRMTLTEASPA
                     LVTYQALFTTILSAALLPFAWETPGWGDLLIMAGIGLCSGIGQFWWTQAFRFAPAAVL
                     APFSYLSMAWSLLLGYLIWGDVPTAGLIGGALVVAASGLYILYRETVRRAPSSATAPA
                     GSD"
     gene            5153..6007
                     /locus_tag="VGP50_12870"
     CDS             5153..6007
                     /locus_tag="VGP50_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEV8028318.1"
                     /translation="MAPRTTWRGHLVLARLSCPVRVHLAVVRSDRLGLQGLNRATLNR
                     LQMRPHDPETGKEVARDFIVRGYEVEPGQFVLLEDRELAGLQIESSRTLVLERFVERS
                     SLDTAYFDTPHFLVPEGRGADAAFGIIRDAMLRQNLVAISRIVLGSRERAVVIETRGK
                     GMLLTTLRAAHEVRSDESYVAEIDQSVADESATGLAERVISRLVGSFDPRRDVRDRYQ
                     EALFHFLQAKRKGEKPVRPAPSVPVAAVDPREALEASVAAMEQAMAAPPGSGATSRRA
                     SRRTVRSR"
     gene            6090..7457
                     /locus_tag="VGP50_12875"
     CDS             6090..7457
                     /locus_tag="VGP50_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV8028319.1"
                     /translation="MSAQVFHRRPTLMQLAADLGAGRTSSAALVEEALARISDPAGEG
                     RRCFIAVYADQARKAAAAQDALRQAGYAASPLAGLPVSIKDLFDVAGEPTLAGSRALD
                     DAPPALHDAPVVARLRAAGAVLIGRTNMTEFAFSAVGINPHFGTPGNPYDRRCIPGGS
                     SSGAGVSVADGFAAVAIGSDTGGSVRIPAALCGVVGFKPTQRRIPREGAVPLSTTLDS
                     IGPLANSVACCALTDAVLAGEAPVLPPEIPVDAVRLGVPRMLVLDDLDAPTAAAFERA
                     CTLLARAGARLVDLPLGEFSEYMAINAKGGFAPPEAYAWHAGLIARRGSDYDPRVRQR
                     IERGSKMSAAEYVALSWQRRDFIARVETSTAAVDALLMPTTALVAPPIAAFDKDEDYW
                     RLNSRLLRNTAIINFLDGCAVSLPIHPPGTAPVGLMIAARNGQDRRLLGIALGIEATM
                     GRARG"
     gene            complement(7489..7854)
                     /locus_tag="VGP50_12880"
     CDS             complement(7489..7854)
                     /locus_tag="VGP50_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEV8028320.1"
                     /translation="MAAVLRLGEYRRRHKTVFFSRPELNRLLSLYSRQVARGEWRDYA
                     IDQRDDAALFSVFRHTQEAALYTVVKTAPEGGKRSAFALLRGRQLLASGPGLGEVLKK
                     LRPAVLLDGVFGAHGTPGG"
BASE COUNT         1424 a         2707 c         2862 g         1204 t
ORIGIN      
        1 gacgacgccg acggcgaaga cgccacaacc gacgagtccg acgacgaggg tggtgacgat
       61 cgcgaccagg aggcgaagcg agcgcagcgc cagcagcagc cacaagcaga ggaggctcag
      121 cgacagtgtc gtcgagaggg ctgcgccctt ggacaaggtc gagaattcgg cgtccgacag
      181 ggcgaccgag ccggtcacgc gcacgcggat gccgcggtcc ggcgtgagcc cgagcgcgag
      241 cgcggcgtca tggatggcgc cggtggcgct tgctccgggt tggagctcgc ggtagtcgag
      301 gatgggttgc gcaaggacga tgcggcgcag ctcttcgggc tcctgcttct ggccggtcag
      361 caggctggcc caggaaagcg ggtcgtagcg cccctcgagc gccgcctcgg tcgccgccgc
      421 ggccgtgttg aaggcagctt cgagctgagg cggtgggtcg ctttcacgga ccgcgccttg
      481 cgcggcgagg tcgagcgcgt cgaacacgcc gcgcaagctc gggtctgcgg cgagcgtgcc
      541 gattagcggc tgcgccgcga cgatctgatc ggcaaaaatc tgcacgtcgg cttgcgacag
      601 gaagaggagg ccgttctgct cgaagaagcg gccgccatcg ggttgccgca cgctcttgaa
      661 gagcgtcttc tgctccgcga gccgcgcggc cagcgccgcg ctcgcgtcct gggcctcatc
      721 gacggttcga gcatcgatca cgacggcgag caggtccgca ttctgcggga atgcctgatt
      781 gagcttccgc tcctgctgac gccatggcag gttcggattg atcagcttgt cgatattggt
      841 atcgaggctg atgtttcgcg cgacgaagta gccgccgatg accgacagca gcagcagcgc
      901 ggcgaccagc ggcgcggcat gacggcggct cgtctcgacc aggcctgcga tcagcgccag
      961 catccgctca cgcctcgcga tagctcaaga gaatgagacc gacgagcacg aaggccacgg
     1021 gccacaccca ggcagcgatc cggctgccgc gcccgtcgag cctgagctcg agccagcgcg
     1081 accagccggc cgtgatcccg cacagcgcca gcggcacatg gctgatctcg atgagcagct
     1141 ggtccttgag gttggcgagc gcatgggagt gggtgaggag caacgtgccg cccagcgcgg
     1201 ttgtcagcgg aaagacgagc gccgcgccgg gccggcgcaa tcggccggtg cggacgcgcc
     1261 attcgaaaag agcgaaggcg acggtgagga gcacgaagat gcggtgctgc accacctcgg
     1321 gatcgcgcaa gctggcgatg aatccgaccg ggccgagcgg ccacacttcg ggatcggagc
     1381 gcaggaagag aaaagcggcc agacccagga acacgagcgg ccagtgccgc gcccagggcg
     1441 cccgacccgt gcgctcgagc aaggccagca ggccgatcgc cagcacgaag agcccggccc
     1501 aatggtggtt gtattcggac caggcgatat cctcggcgtt gcgcggtgga gcgatgcctt
     1561 cgccggggac gtaagcttcc ggccgcggcg cgtccgtgcg gactgtgcca cggagctgtg
     1621 catcgagttc cgagagagcg aggcttgcat agtcggggct ttcgaaccgc ggccagtgcg
     1681 gcgtcagccg ctcgacgata tcgtggaggc tggcgcggtc cgtcgtgagg tcgacagcgg
     1741 gcggttgcga ggtgagcgaa gcggcggcga agagtacggc caagccgacg ccgatctcga
     1801 cttcggcaaa ccgcctcagg cgcaggatgg gcgtcctcgg atcgcgccgc aaccgctcga
     1861 cgacgcgata attcatcgca ccgaggaaca agaggccgac gagaagcagc accttggtgc
     1921 cgaccatcac gccataggcg gtgccgtaga acgcctcgag cgagccgata tatgtgaacg
     1981 ccatgacgat tccggcgccg acgaggatcg cgacggctcc catggcgatc aacgagaagc
     2041 gccggccgat gcgcgcctcg tcggtggggt tgcgcatttg ctgcaaggtg agcagcaagt
     2101 aaggaatccc gccgatccag gcggcggcgc ccgccatatg cgcgaagtcg gcgatcagca
     2161 gcggcgcgcg gccgttcaac tgcgcggcgg cgtggctgct cgccacctgc gccaccagga
     2221 tggtgatccc gagcagcggc aagggcgaaa agcgaccggt cgcaatcggc cgtcccgtca
     2281 gcgcagccgc ggccaatgac gccgccatgg cgacaatgcc gaggcgcgca aaatcggcgc
     2341 ccaacgcatc ggcgagcgag atgtcgatcg ttccggcaag gacactcgct tcggtgagca
     2401 cgaagaccag ctcgacgccg gcgaggacca cggcggacca gcgcagcaac gcatgacagc
     2461 gccggcgcgc cgccgcaccg tcgctcccga ggcgcggcgt cagcggatcc aggagcaggg
     2521 acaggaacac gatgccgccg atcgtcagcg actgtgccgt cagcacgact ccgcgcagga
     2581 tgacactgag aaagccgaag atgtcgagca gcagcgccat cgatcggtca gggagcgacg
     2641 gtgaagggga tgtcgccgcg cgtaatgtgg ccatcgaccg cgaggacttg ccagcgcagg
     2701 cggtaagcgc cggagccgag cccatccatc gtcgcggcga gcgtatcggg accgtcgctg
     2761 ggcgcgagcg ccagcggccg cgtctcgccg tccggcgata ttagcgtcag ccgcgagcgc
     2821 tcgacgtcga tgcggctgtt gaaacgcagc agcacctgcg ctttcgaccc gtggaggaca
     2881 gcaccggccg caggctcggc ggcgacgatg atggcatggg cgagagccga caccggcgcg
     2941 gcgagcagca aggcagcgag gccgggggca aaagcgagcc gcataggcgc cctttattga
     3001 ggaccgaccg cccgggcgca agctgaaatc gcggacaggg ccgggtccgt gactcagaac
     3061 ttgtagccgg tgcgcaaccc caaattcgtc atgccggcat tgtgcgtggc gagatcggca
     3121 ttcgacatgt ggtcgaggaa ggccgagagg ctgaagcggc ggctgatgcg ccagccgccc
     3181 tcgacgctct cgtgaaagag gatgcgcgaa cccagctcct tgcgatcggg gatatccttg
     3241 tcgagatcgc cgtcatgcac cgcgccgccg aggccgaggc cgaaaaacag cggtccgtcc
     3301 cagggcgcca gcgtccaggt cagcccgaaa tagccgtaac tcgtggcccc cgccgtattg
     3361 agggagccgc cgagatgggg ccggggcgcg ccgatcgcct gcaggaagtc aggcgacgcg
     3421 aatagcagtt cgccgttgat atccggcccg ggctcccggt gatcgccgcc gatggggatg
     3481 tcgtgataca tgaggccgag ctttccttcg tcgagccatc cctctgcctg cgccggtcga
     3541 gacaaggcaa ggatcgcggc caggggcagc atcgccgctg cctggcgcca attgacaccg
     3601 gccggagccc tctgctgctg tcgatggatg agcccttcgc cgcgcgcggc gcgatgacgc
     3661 gcgagcagat gaacatggaa ttgcagcggg gccggctcga gcgccgcaag cccgtcgtct
     3721 ttgtcacgca ttcgacctcc gatgcggtgt tcctggccga tcgtgtgccg gtagtggcct
     3781 cacggccggg tcgcatcgcg gaagtcgtct cgatcaacaa cccacggcta cgcgcgcccg
     3841 gagttttgaa cgctggcgca ttcggcgagt ttcttccagc ctatccagac ggatgtcgag
     3901 gcgacacgaa aggataggat gcgagccatg tcgatggcgt ggccttcggc cggggcggtg
     3961 gcttaatccg agccggccgg agccgttgcg gaggaggggg cgcgacgcac cgtctcgcga
     4021 tagaggatgt agagtccgct tgcggcgacc acgagggcgc cgccgataag gccggcggtc
     4081 ggcacatcgc cccagatcag gtagccgagg agcaacgacc atgccatcga gagatagctg
     4141 aagggcgcca agaccgcagc cggagcgaaa cggaacgcct gagtccacca gaactgaccg
     4201 atccccgagc agagcccgat ccccgccatg atcaagaggt cgccccagcc cggcgtctcc
     4261 caggcgaaag gcagcagggc cgcgcttaga atcgtcgtga agagcgcctg gtaagtaacg
     4321 agggcagggc tcgcctccgt cagcgtcata cggcgaaccg cgatcgtcac ggaggcgccg
     4381 atcgccgcgc tcatcagcgc caggagcgcg ccggtcgagg caaggccccc ggcgaagccg
     4441 ccgccgccgc ggaccatgag aaggacgccg acgaaaccga tcagcaccgc cgcccaacga
     4501 tgccggccga ctcgctcgcc gagcagcggg atcgacaaca gcgtgaggaa aagcggggag
     4561 gagaaggaga tggccaccgc gtcggcaagc ggcatcatgg cgaaggcggc gaagatgctc
     4621 aggatcgaga tgaactgcat gacggcgcgg ccgatatgct cgcgcagccg ccgcgtccgc
     4681 atgagccgga agccgccatg cgcggcgatt agcgccatgc acggcaatag cgaaaagacg
     4741 cagcgcagga agacgacctc gtcgagcggg tagcgcgccg cctcccattt cacgccggca
     4801 ttgatggcgg agaacaccag gacggcgccc atcatgtaga gaatgcctgc ccggacctcc
     4861 tcgcggctcg gcaccggcat ggcaaagcgt cgcggcgtcg ccttcgccgc cacgtcctct
     4921 ccctgccatt cgatcgtccg cgtcatcgag gtcgtcgcct ccaagcgctt cgtcataacg
     4981 tgaagtgctt tacggccgag ttaactggcc ccctaattgg ccgacgaaga tggcgggtct
     5041 tgggcgaaat tgggatgaaa ctcgtgcaac cttcggttcc ctcgcacgtt aacgccagcg
     5101 gttgcccgcc cgtcgggccg ccgcggagaa gacgatcgcg gagaagacga tcatggcgcc
     5161 gcgaacgacc tggcgagggc atctcgtgct cgcccgcctg agctgcccgg tgcgggtcca
     5221 tctcgcggtg gtgcgcagcg atcgcctcgg tctccagggg ctcaatcgcg cgacgctgaa
     5281 ccggctgcag atgcgcccgc atgatcccga gaccggcaag gaagtggccc gcgacttcat
     5341 cgtgcgtggc tacgaggtcg agcctggtca attcgtcctg ctcgaggatc gcgagcttgc
     5401 cgggctgcag atcgagtcgt cgcgcacact cgtgctcgag cggttcgtcg aacggtcctc
     5461 gctcgacacg gcatatttcg atacgcctca tttcctggtt cccgaagggc gcggcgccga
     5521 cgcggcgttc gggatcattc gcgacgcgat gctgcgtcag aatctggtcg ccatcagccg
     5581 gatcgtcctt ggcagccgcg aaagggccgt cgtcatcgag acgcgcggta aaggcatgct
     5641 gctcaccacc ttgcgggcgg ctcatgaggt ccgcagcgac gaatcctatg tcgccgagat
     5701 cgaccagagt gtcgccgacg agtcggcgac gggcctggcc gaacgcgtga tcagccggct
     5761 tgtcggctct ttcgatcccc ggcgcgacgt ccgcgaccgc tatcaggagg cgctcttcca
     5821 cttcctgcag gcgaagcgca agggggagaa gcccgtccgt ccggcaccat cggtcccggt
     5881 cgccgccgtc gatccgcgcg aagcgctcga ggcgagcgtc gccgccatgg agcaagcgat
     5941 ggcggcgccg ccgggaagcg gtgcgacatc gcgccgcgcc agtcgaagga ccgtgcgatc
     6001 gcgctgacac cgggggccaa gaggcgcgga gtcgcagccg cgtatttgcc gcgttaaagt
     6061 gatcctcctc cgcgtcagga ggatcgccga tgtccgccca ggtctttcac cgacgaccga
     6121 cacttatgca gctcgccgcc gacctcggtg ccgggcgcac cagcagtgcc gccctggtcg
     6181 aggaggccct ggcgcggatc tccgatcccg caggcgaggg ccggcgctgc ttcatcgccg
     6241 tctacgccga ccaggcgcgc aaggccgcag cggctcaaga cgcgcttcgc caggcgggat
     6301 atgccgcatc gccgctggca gggctccccg tctccatcaa ggatctgttc gacgtggcgg
     6361 gcgagcccac gctggccggc tcgcgggcgc tcgatgatgc gccgccggct ttgcacgatg
     6421 cgcccgtcgt cgcgcgcctg cgggctgccg gggccgtcct gatcggccgt accaacatga
     6481 ccgaattcgc cttctcggcc gttggcatca acccgcattt cggcacgccg ggcaatccct
     6541 atgaccggcg ttgcatcccg ggcggctcat cctcgggcgc cggcgtttcg gtggcggacg
     6601 gctttgccgc tgtggccatc ggctcggaca ctggcggctc ggtccggata cctgcggcgc
     6661 tctgtggcgt cgtcggcttc aagccgacgc agcgtcgcat cccccgggag ggcgccgttc
     6721 cgctctcgac gactctcgac tcgatcgggc ccctcgccaa cagcgtcgcc tgttgcgccc
     6781 tgacggacgc ggtgcttgcg ggcgaggcgc cggtcctgcc gcccgagatc ccggtcgacg
     6841 ccgtgcgcct cggggtaccg cgcatgctgg tgctcgacga cctcgacgcc ccgaccgccg
     6901 cggccttcga gcgcgcctgt accctgctgg cgcgcgccgg tgcgcgcctc gtcgatctgc
     6961 cgctcggcga gttctccgaa tacatggcga tcaatgccaa gggcggcttc gcgccgcccg
     7021 aggcctatgc ctggcatgcc gggctgatcg cccgccgcgg cagcgactac gatccccgcg
     7081 tccgccagag gatcgagcgc ggcagcaaga tgagcgctgc ggaatacgtc gcgctgtcat
     7141 ggcagcgccg cgacttcatc gcccgggttg agacgagcac ggccgccgtc gatgcgctcc
     7201 tcatgccgac cacggcgctg gtcgcgccgc cgatcgccgc cttcgacaag gacgaggact
     7261 actggcggct caattctcgt ctgctgcgca acacggcgat catcaacttc ctcgatggct
     7321 gcgccgtgag cctgccgatc catcctccgg gaacggcgcc ggtcggcctc atgatcgccg
     7381 cgcgaaacgg ccaggaccgg cggctgctcg gcatcgcgct cggcatcgag gcgacgatgg
     7441 gccgcgcccg cggctgacgg cggccttgat ctcgcagagc ggcagcggtc aaccgccggg
     7501 cgtgccgtga gcgccgaaga cgccgtcgag aagcaccgcc ggccggagct tcttgagcac
     7561 ttcgccgagg cctgggccgc tggccaggag ctggcgcccg cggagcaggg cgaaggcgct
     7621 ccgctttcca ccctctggtg ccgtcttgac gaccgtgtaa agcgccgcct cttgggtgtg
     7681 gcgaaagacc gagaacagcg ctgcgtcgtc gcgctgatcg atcgcataat cgcgccattc
     7741 gccgcgggcg acctgacgac tatagaggct caagagccgg ttcagctcgg gccggctgaa
     7801 aaagacggtc ttgtgccgtc gccgatactc gccgagccgc aggacggccg ccatcggccg
     7861 tgtctcttgg ttggtgctca ttcgttcgat gcggaaataa gtgttcctca tttccggccg
     7921 caggtctagc ccggatttcc ggcacgggga tgctgtgcgt tgcgccgtgt tggcgcgatg
     7981 tcagaagcga agcattttag atgccggacc ccgcgaaggc cgtcgcgacg cggcggaaat
     8041 ccgggcgagc ggtttgccta ggaggggccc agccgctatg gtagcggccg cgtgcgcgcc
     8101 cggggtgttt cgtgagtgat attttccagg aaatcgacga ggaactgcgg cgcgagaact
     8161 tcgccaagct gtggcagcgc tacggcatct atgtgat
//
LOCUS       DASYNS010000417        10286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2916267, whole genome shotgun sequence.
ACCESSION   DASYNS010000417 DASYNS010000000
VERSION     DASYNS010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10286
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2916267"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="VGP50_12885"
     CDS             complement(<1..1511)
                     /locus_tag="VGP50_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEV8028321.1"
                     /translation="MLLFALAYLGGALTIVSPCILPVLPFVFAGADRPFLRRGLPLLA
                     GMAATFAVIASLAAVGGGWAVAANQYGRFAALVLLALFGVTLLVPSLAERMTRPLVAL
                     GASLSQTADQSGGKGGVLPSLLLGVATGLLWAPCAGPILGLILTGAALNGATVGTSVL
                     LLAYAAGAATSLGAALLVGGRLFAAMKRSLGAGEWIRRGLGVAVIAAVVAIALGADTG
                     FLTNVSLAGTAALEQRLVDKLDPAAAPGHPPAAASGGPAMMSGGPATMTGDNPAMMSG
                     GPAMTAADNPAMMMKAKPPAESEALPVEGALPSLAGAVAWLNSPPLSEADLKGKVVLV
                     DFWTYSCINCLRTIPYIRAWAEKYKDQGLAVIGVHAPEFAFEKKIDNVKKAVADLGIA
                     YPVAIDNDYAIWRGFHNEYWPAHYFIDAEGRIRHHHFGEGEYDESERVIQLLLAETGK
                     TALPAGLVTVNASGAEAAPDMSDVRSPETYVGYARAENFVSPGGAVHDMSHVY"
     gene            complement(1531..2025)
                     /gene="msrB"
                     /locus_tag="VGP50_12890"
     CDS             complement(1531..2025)
                     /gene="msrB"
                     /locus_tag="VGP50_12890"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV8028322.1"
                     /translation="MLTRRAVLSGGICGFAVLSGGAVLAPRIAWSAAEDHVTHTDEEW
                     RELLTPDQYAVLRHEGTERPFTSPLLHEARRGDFACAGCDLDLFSSTTKYDSRTGWPS
                     FWAPLENAIVTKPDHSFGTTRTAVMCRRCEGHLGHVFDDGPQPTGLRYCMNGVALTFK
                     PVAA"
     gene            2425..3327
                     /locus_tag="VGP50_12895"
     CDS             2425..3327
                     /locus_tag="VGP50_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEV8028323.1"
                     /translation="MTLEQLRVFVAVAERQHVTRAAAALNLAQSAASAAIAALEAQHG
                     ATLFHRVGRGIELTEAGRLFLVEARAVLARAEAAELVLSELGGLKRGTLTVQASQTIA
                     GYWLPRHLVAFRRAHPGIDIRLAVGNTAQVARAVHDGAADIGFIEGDIDDPLLTVELV
                     AHDQLVIVVGAEHPWARDGGLDSARLTESEWVLREPGSGTRSAFEAALPKLGLTSGML
                     RIALELPSNEAVRAAVEAGLGATAISGSVAAPGIEAGLLHQVPIDLPQRNFCVVHHVE
                     RHRSKAADALLAMVTTLFPPRRGV"
     gene            complement(3755..4474)
                     /locus_tag="VGP50_12900"
     CDS             complement(3755..4474)
                     /locus_tag="VGP50_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012226865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8028324.1"
                     /translation="MTLKDKRIIVLGGTSGIGLAVAAVAAADGARVLVASSDDARVKR
                     ALTTLPVGAEGRVLDLSNATAIAAFFDAVGPFDHLVYTAGESLKLSPLADGDIASARH
                     FFELRYWGAFMAARWGHRQIRTGGSIVFTSGVAGARPRPGWSVAASICAAMEGLTRAL
                     AVELAPVRVNIVSPGIVKTPLWREMPEETRESLYASEARRLPVGHIADPQEIAEGYLY
                     LMRQTFVTGQTLAIDGGGLLA"
     gene            4571..5668
                     /locus_tag="VGP50_12905"
     CDS             4571..5668
                     /locus_tag="VGP50_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HEV8028325.1"
                     /translation="MLDADVRSAVALRCQCDDRRGRSRQLQTAMMPKFRQIQPNRQMQ
                     PASRLIEMVAFEAVQLLDVTGPLQVFATANDTVADTGAPPPYTLRIVARQTPNVTASA
                     GIVLATGDLSSLDTPLDTLMIAGGAGVKAASADPGLVRWVRARAETARRTASVCSGAF
                     LLAAAGVLDGRRAVTHWSFCDELAQCFPAVHVESDPIFVRDGAVWTSAGVTAGIDLAL
                     ALVEKDLGRTVALAVARYLVMFLKRPGGQAQFSAALSLQAGEERFGPLHDWINANLSR
                     DLPLSVLAAQAGMSERSFSRHYTEATGLTPARAVERLRLEAARQQLLESKVPVKRIAQ
                     HCGFGTEETMRRSFLRLLSTTPQDYRARFGM"
     gene            5886..6434
                     /locus_tag="VGP50_12910"
     CDS             5886..6434
                     /locus_tag="VGP50_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028326.1"
                     /translation="MVSFPVYTIDSAPDAAKPALRDLKAAFGMVPNIAGAMATSPVLI
                     NSLVGLFGRVHGGSFTEAQIQTLLLTNAVTNACRWAVAFHTALALKQGLDPADVDAMR
                     AGRSPKDGKLGALSTLARTLIEKRGRIDDQEAEQFLAAGFGKDHLLDVIAVVAASTIT
                     NYTGSVTNPPLEAPFQAHAWGG"
     gene            6463..7119
                     /locus_tag="VGP50_12915"
     CDS             6463..7119
                     /locus_tag="VGP50_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEV8028327.1"
                     /translation="MKIYDWPTGPYPARVRIAVAEKNLQSRVQFERVNLWKGEHKTAE
                     FLAKNYSGTLPVLELDDGTFIAECTAITEYLDALDGAPTLPGRTAREKGLIHMMTKRA
                     ELELLDAISVYFHHATPGLGPDVETYQNAEWGFRQRDKALKGMHYFDAILKRQPFVAG
                     EVFSMADITVIGGLIFVGLLELPVPAACAALQAWYGKMQERPSVKNRVTMSRPTETAV
                     "
     gene            complement(7320..9473)
                     /locus_tag="VGP50_12920"
     CDS             complement(7320..9473)
                     /locus_tag="VGP50_12920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV8028328.1"
                     /translation="MYKDFAGLPSVPASERRPTVCIVSGEVVGPFKNGGIGTSMTGLA
                     ESLAGAGFAVSILYTGGVWVPDVDLGEWRERYRGIGIGFTALGFADMARLDGPLRQRG
                     FGVPLLVYDHLRSNRFDVVHFNDCVGDGYYCLVMKRLGVAFQDSLLCLALHSPSQWVL
                     EHNQRLPDSILYAAYNYAERLSTRAADLLWAPSRYIIEWAKSKGFVLPAATYFQQYVI
                     PTADLFRADPEKFTASPPRSDAIVHPKEIVFFGRLEERKGLRVFCNAMERLDGVLAAK
                     DIAVTFLGKSAPVAGTDSLDYLARRGAGWHFRWQTVTDLGQQEAIRYMLSRPALAVMP
                     SPVDNSPCTIYEALGFGIPFIAARTGGIPELIDPADRDEALFASSATALADKLAQVIA
                     RGARVARPAIAQRIARRRWVDAHRAWRTLLPRPERAAAAARGLVAIVDHGVGSDLDAT
                     LASLRRLPMVRRIVVLNRSPAPVAAALPDPRLREISLFVDEPDTIFAELPDGDSVAVL
                     LLQSGVTVLPEAVPEMLTSLQHEEIDGLVPAARVGGSGAGTIVPPLGGSPAFSFFEGI
                     GFTGGLVIKAERFRRAVMGKLLALETEFLGLADHAVAAGLELWPYVEPVFCHPEGYQR
                     RRAGFGAPARIAAYDQVSPTERYYIAALGYAAVERPGPPATAFRREVRLRLAELHLGW
                     LVAVALRLLSRRQIDAIAPRLRRLRWLLPKRPTRS"
     gene            complement(9481..>10286)
                     /locus_tag="VGP50_12925"
     CDS             complement(9481..>10286)
                     /locus_tag="VGP50_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028329.1"
                     /translation="DPILSDPLHRDSVEHALRCCGVRDAVTLHGAASPAAIAALAAKD
                     GAVWELFVVDGDHEPPMPERDVDSCVAHAADDAAFVLHDLASPEVAAALRLLEARGFN
                     VMVYQTMQIMGIAWRGQVTPVHHTPDPAVAWQLPHHLVGLPVSGAAFSGYPADLRARA
                     IRQEQEIHRLDRGGVQRETEIRRLDEELRQRDRDIERLSEETQRRDRDMQRLSEEGQQ
                     RDQDMRRLAEAIQQRDRDIERLGRELAAANARLTHRLYRRLRRLLSLGQ"
BASE COUNT         1753 a         3391 c         3544 g         1598 t
ORIGIN      
        1 tcatagacat ggctcatgtc gtgcacggcg ccgccgggcg agacgaagtt ctcggcgcga
       61 gcgtagccga cataggtctc gggcgagcgc acatcgctca tgtcgggtgc ggcctcggcg
      121 cccgaggcgt tgaccgtgac cagcccggcc gggagcgcgg tcttgccggt ctcggccagg
      181 agaagctgaa tgacgcgctc cgattcatcg tattcgccct cgccgaaatg gtgatggcga
      241 atgcggccct cggcgtcgat gaaataatgc gccggccaat attcgttgtg gaagccgcgc
      301 cagatcgcat agtcgttgtc gatcgcgacg ggataggcga tgccgagatc ggcgacggcc
      361 ttcttcacgt tgtcgatctt cttctcgaag gcgaattccg gcgcgtggac gccgatcacc
      421 gccagaccct gatccttgta tttctccgcc caggcgcgga tgtaggggat cgtccgcagg
      481 cagttgatgc aggaataggt ccagaagtcg acgaggacga ccttgccctt gaggtccgct
      541 tcgctcaacg gcggcgagtt cagccacgcg accgcgccgg cgagagaggg cagcgcgccc
      601 tcgaccggca gagcctcgga ctccgccggc ggcttcgcct tcatcatcat ggcgggattg
      661 tcggccgccg tcatggccgg accgccgctc atcatggcgg gattgtcgcc cgtcatcgtg
      721 gcggggccgc cgctcatcat cgccgggcca cccgacgccg cggccggcgg gtgccccggc
      781 gctgcggccg gatcgagctt gtcgaccagg cgctgctcga gcgcggccgt gccggcgagc
      841 gacacattcg tcaggaagcc ggtatcggcg ccaagcgcga tcgctaccac ggcggcgatc
      901 accgccacgc cgaggccgcg gcggatccac tcgccggcgc cgagcgagcg cttcatcgcg
      961 gcgaagagcc gccccccgac cagcagcgcc gcacccagcg acgttgccgc gccggcggca
     1021 taagcaagga ggagaacgct ggtcccgaca gtcgcgccat tgagcgccgc gccggtcagg
     1081 atcaggccca ggatcggccc ggcgcagggt gcccagagca gcccggtcgc gacgcccagg
     1141 agcagggagg gcaggacgcc gcctttgccg ccgctctggt ccgccgtttg ggagaggctt
     1201 gcgccgagag cgaccaaggg acgcgtcatc cgctcggcga gcgacggaac gaggagcgtc
     1261 accccgaaaa gcgcgaggag gacgagggcc gcgaagcggc catattggtt ggcagcgacg
     1321 gcccagccgc cgcccactgc cgcgaggctg gcaatgacgg cgaaggtcgc ggccatgccg
     1381 gcgagcagcg gcaagccgcg gcgcaggaac ggcctgtcgg cgccggcgaa gacgaagggc
     1441 agcaccggca ggatgcacgg gctgacgatc gtcagcgcgc cgccgagata ggcgagggcg
     1501 aacaggagca tcgcttccgg cctcgcttcg tcaggcggcg acaggcttga aggtcaacgc
     1561 gacgccgttc atgcaatagc gcaagccggt gggctgcggc ccgtcgtcga agacatggcc
     1621 gagatggcct tcgcagcgcc ggcacatgac ggcggtgcgg gtcgtgccga aagagtgatc
     1681 gggcttggtg acgatggcgt tctccaaggg cgcccagaag ctcggccagc cggtgcggct
     1741 gtcgtatttc gtggtcgagg agaagaggtc gaggtcgcat ccggcgcagg cgaaatcgcc
     1801 gcgccgcgcc tcgtggagca gcgggctggt gaacggccgc tccgtgcctt cgtggcgcag
     1861 cacggcatat tgatcgggcg tgaggagctc gcgccattcc tcgtcggtat gggtgacgtg
     1921 atcctccgcg gcggaccagg cgatccgcgg cgcgagcaca gcgccgcccg acagcaccgc
     1981 aaatccgcag ataccgccgc tcaagacagc tcgacgtgtc agcatgctcc tcactccgtc
     2041 gtgtttggcc ggacgctcgg ctcgtcccac gtcactgttc gctgctgcag ccattttggt
     2101 tacccgggga tggccgtccg cgagactagc acagggaatc cgagaaacga gatgaccgac
     2161 aagcgctgga aagcccgagc tcgatcactc ccgcgtgctc cttgggacgc ggctccggcg
     2221 cggcgctcga tctggccgaa aggggactgg cagctgtcga gagtccgtca ggcgaggccc
     2281 agcagccggg tcagcccacc gatccacgac gcgatcggtg atttcgcggc cattcgccgc
     2341 cgcgatcatt ccgagcacaa agccgagctg acggcaggcc ggcccgtgta gccgccccgc
     2401 gcgccgtgtg gcagcatgca gcgcatgacg ctggagcagc tacgggtctt tgtcgcggtc
     2461 gcggagcggc agcatgtcac gcgtgcggcc gcggcgctga acctcgcgca gtccgcggcg
     2521 agtgccgcca tcgcggcgct cgaggcgcag catggcgcga cgctgttcca ccgggtcggc
     2581 cgcggcattg aattgaccga ggccggcagg ctgtttctcg tcgaggcgcg cgcggtgctg
     2641 gcacgcgccg aagcggctga attggtgctg tcggaactgg gcggcttgaa gcgcggaacc
     2701 ctgacggtac aggccagcca gaccatcgcc ggctactggc tgccacgcca tctggtggcg
     2761 ttccggcgcg cgcatcccgg catcgatatc cgcctggctg tcggcaatac ggcccaggtc
     2821 gcccgcgcgg tgcatgacgg cgccgccgac attggtttca tcgaaggaga catcgacgat
     2881 ccgctgctga cggtcgagct cgtcgcgcat gatcagctgg taatcgtcgt cggggcagag
     2941 catccgtggg cccgggacgg cgggctcgac tccgcacggc tcaccgagag cgagtgggtg
     3001 ctgcgagagc cgggttccgg gacgcggtca gctttcgaag cagcgctgcc gaagctcggc
     3061 ctgacgtccg gcatgctgcg gatcgccctc gagctgccgt ccaatgaagc ggtgcgcgcg
     3121 gcggtcgaag ccggtctggg ggcgaccgcg atctcgggct ccgtcgcggc gccgggcatc
     3181 gaggccggac tgttgcacca ggtaccgatc gaccttccgc aacgcaattt ctgtgtcgta
     3241 caccacgtgg aacgtcaccg cagcaaggcg gcggatgctc tgctggcgat ggtcaccacg
     3301 ctgtttcccc cgcggcgcgg ggtctgaggg cgccgacatc gatcgcggaa agcgatgaaa
     3361 tcgagcgtcc ttttgcgata gagctgggcc aggcccgcga tcactttcca caccgacgcc
     3421 gtccaatggc gcgtggagac cggtctcatg acctcggtgc tccgaccgaa cagaatggtc
     3481 cgcgatcagg tttcatcagg ggatcccgtc tcgggccagg actgtcgcat atggggttca
     3541 ccacggaggc acggaggaca cggagaagag gggttcggcg cgcgaagcgc gccatccttc
     3601 gcttctccgt gctctctccg tgcctccgtg gtcaatcttg accgcacagg aactcgactt
     3661 catccgcgca gccgcgttga ttagggcggc tttccactat cccggtcgac tggccccggg
     3721 gatgtcttgt ccgctttcgg gaagtcgacc gcgatcaggc caggaggccg ccgccgtcga
     3781 tcgccagagt ctggccggtc acgaatgtct ggcgcatcaa atagagatag ccctcggcaa
     3841 tctcctgcgg gtcggcgata tgccccaccg gcaggcgccg cgcttcggac gcatagagcg
     3901 actcccgcgt ctcctccggc atctcacgcc acagcggtgt cttgacgatg cccggggaga
     3961 cgatgttgac gcggaccggc gccagctcta ccgccagggc tcgtgtcagc ccctccatcg
     4021 ccgcgcagat actcgccgcg acagaccagc ccggtcgcgg ccttgccccc gcgacgccgg
     4081 atgtgaacac gatcgagccg cccgtccgga tctggcggtg cccccatcgg gccgccatga
     4141 acgcgcccca ataacgcagt tcgaagaagt gacgggcgct ggcgatgtcc ccgtccgcca
     4201 ggggcgagag cttcagcgac tccccggcgg tgtagacgag atggtcaaaa ggcccgacag
     4261 cgtcaaagaa agccgcgatg gcggtggcgt tcgagaggtc gagcacgcgt ccttccgcgc
     4321 cgacgggaag cgtcgtcagc gctcgcttga cccgcgcgtc gtcgctggac gcgaccagca
     4381 cccgggcgcc gtccgcggcc gcgacggccg cgaccgcgag gccgataccc gatgttccgc
     4441 caagaacgat gatccgcttg tccttcaagg tcatggcagc ctccttttgc gaaggctctt
     4501 tttctcatcc tgcccgcctt tgacacaagc gcatccgagt ccatacaaag atgactcgga
     4561 gtcacgatca atgctcgatg ccgatgttcg aagcgcggtt gctctccggt gtcagtgtga
     4621 tgatcgccgt ggtcgaagcc ggcagcttca gacggcgatg atgccaaagt tccgacagat
     4681 ccagccaaac cggcagatgc aaccggcgtc gcggctgatc gaaatggtcg cgttcgaagc
     4741 cgtgcagctg ctcgacgtca cggggccgct gcaggtgttc gcaacggcca acgacaccgt
     4801 cgccgatacc ggcgcgccgc cgccctacac gctccggatc gtcgcccggc agacgccgaa
     4861 cgtgacggcg tcggccggaa tcgttctcgc gacgggcgat ttgtcgtccc tcgacacgcc
     4921 gctcgacacc ttgatgatcg ccggcggcgc gggcgtcaaa gccgcttctg ccgatcccgg
     4981 gctggtgcgt tgggtccggg cccgcgcgga gacggcgcgg cgcaccgcct cggtctgcag
     5041 cggcgccttc ctgctggcgg cagcgggcgt tctcgacgga cgccgcgcgg taacgcactg
     5101 gtcgttctgc gatgaactcg cgcagtgctt tccagccgtg catgtcgagt ccgatccgat
     5161 tttcgtgcgg gacggcgcgg tctggacatc ggcgggcgtg accgccggga tcgaccttgc
     5221 cttggcactg gtggaaaagg acctcggccg aacggtggcg ctggccgtgg cacggtacct
     5281 ggtgatgttc ctcaagcggc cgggtggaca ggcgcagttc agcgcggccc tctcgctcca
     5341 agccggcgag gagcggttcg gcccgctcca cgactggatc aacgcgaatc tcagccgcga
     5401 tctgccgctg tccgtccttg ccgctcaggc cgggatgagc gagcgcagct tcagccggca
     5461 ctacaccgag gcgaccgggc tcacgccggc ccgggcggtg gagcggctgc ggttggaggc
     5521 ggcgcggcag cagcttttgg agtccaaggt cccggtcaag cgcatcgcgc agcattgcgg
     5581 cttcgggacg gaagagacga tgcggcgcag ctttctgcgc ctgctatcga cgaccccgca
     5641 ggattatcgc gcccgcttcg gcatgtagcg gtcgcgcgat ttggtcgccc tggacgccga
     5701 tgacgccccg tggcgcagaa ggtggggagt ttggcgagca cacatcgccg cgcaggccca
     5761 aataaactgc gtaagacgtg ccggcccaat atcgcttcgg ccgttgtcgc ggttacacgg
     5821 cggcggacgg taaggggcag tcaggcgcgt cgcgcttgct caacccttcg aaaaggcaaa
     5881 atcacatggt ctcctttccc gtctacacga tcgactccgc cccggacgcc gcgaagcccg
     5941 ctttgcgcga cctcaaggcg gcgttcggga tggtccccaa catcgccggt gccatggcga
     6001 catcgccggt gctgatcaac agcctcgtcg gcctgttcgg ccgggtccat ggcggcagtt
     6061 tcaccgaggc gcagatccag accctgctgc tcaccaacgc ggtgacgaac gcctgccgtt
     6121 gggccgtcgc gttccatacc gcactggcgc tgaagcaagg cctggatcca gcggatgtcg
     6181 acgcgatgcg cgccggccgc tcgccgaaag acggtaagct cggcgcgctc tcgacgctgg
     6241 cgcggacgct gatcgaaaag cgcggacgga tcgacgatca agaggccgag cagttcctcg
     6301 ccgccggctt cggcaaagat catctgctcg atgtgatcgc cgtggtcgcc gcctcgacca
     6361 tcaccaacta tacgggcagc gtcacgaacc cgccgttgga ggcgccgttc caggcgcatg
     6421 cgtggggcgg ctgaccggct cgccgggcga aggaggtgga tgatgaagat atacgattgg
     6481 ccgaccggcc cttatccggc gcgtgtccgt atcgctgtgg ccgagaagaa cctgcaatcg
     6541 cgtgtccagt tcgagagggt caatctctgg aaaggcgagc acaagacggc cgagttcctc
     6601 gccaagaact actcaggcac gctgccggtg ctcgagctcg atgatgggac cttcattgcc
     6661 gagtgcacgg cgattaccga ataccttgac gcgctcgatg gcgctcccac gctccccggg
     6721 aggacagcgc gcgaaaaggg cttgatccac atgatgacca agcgcgccga gttggagctg
     6781 ctcgacgcca tcagtgttta tttccaccac gcgacgccgg ggcttggacc cgacgtcgag
     6841 acttatcaga acgccgagtg ggggttccgt cagcgcgaca aagccttgaa gggaatgcac
     6901 tacttcgatg ccattctaaa aagacagccc ttcgtcgccg gcgaggtctt ctcgatggcc
     6961 gacatcacgg tcatcggcgg tttgatcttt gtgggacttc tggagctgcc ggtgcctgcg
     7021 gcgtgcgcgg ctcttcaagc gtggtacggc aagatgcagg agcgtcccag cgtgaagaac
     7081 agggtgacga tgtcacggcc gaccgagacg gccgtttgaa agcgggaatc cagcggcaac
     7141 tatagatccc cgctttcgca aagatgacga gaacacagcg gttatttctc aacagcccct
     7201 tggagtgagc aatcgaacgg cgtgattggg cgcgaaggcg acagccgccg ccgcgctgaa
     7261 tgtccgcttc ccgaaagcac caaggcgtcc gaatggcggt ctcgggcgca atcctgccct
     7321 caagagcgcg tgggccgttt cggcagcagc cagcgcaggc gccgaagccg cggcgcgatc
     7381 gcgtcgatct ggcggcgaga gaggagccgc agcgcgacgg cgaccagcca gccgagatgc
     7441 agctcggcga ggcgcagcct gacctcgcgc ctgaacgccg tcgcgggcgg ccccggccgc
     7501 tcgacggcgg cgtagcccag cgcggcgatg taatagcgct cggtgggcga aacctggtca
     7561 taggcggcga tccgagccgg cgcgccgaag cctgcccgtc gccgctgata gccttcggga
     7621 tggcaaaaga ccggctcgac ataaggccag agctcgaggc cggcagcgac cgcgtgatcg
     7681 gcaaggccga gaaactccgt ctcgagagcg agcagcttgc ccatcactgc ccggcggaaa
     7741 cgctcggcct tgatgacgag gccgccggtg aagccgatac cctcgaagaa gctgaaggcc
     7801 gggctgccgc cgagcggcgg cacgatcgtg ccggcgccgc tcccgccgac ccgggcggcg
     7861 gggaccaggc cgtcgatctc ctcatgctgg agcgaagtca acatctcggg cacggcttcc
     7921 ggcaggacgg tgacgcccga ttgcagcagc agaaccgcta cggaatcgcc gtccggaagc
     7981 tccgcgaaga tcgtgtcggg ctcgtccacg aagaggctga tctcgcgcag ccgcggatcg
     8041 ggaagagccg ccgcgacagg cgccggggag cgattgagca cgacgatgcg ccgcaccatt
     8101 gggaggcgcc ggagcgaagc gagcgtcgca tccaggtcgc tccccacgcc gtggtcgacg
     8161 atcgcgacga gaccgcgggc ggcggcggcg gcgcgttcgg ggcgcggcag cagcgtccgc
     8221 caggcgcggt gggcgtcgac ccagcgccgg cgcgcgatgc gttgggcgat cgccggacgc
     8281 gccacgcgtg cgccccgagc gatgacctgc gccagcttgt ccgcgagcgc cgtggcggag
     8341 cttgcgaaca aggcctcgtc gcgatcggcg gggtcgatga gctcgggaat gccgccggtt
     8401 ctcgcggcga tgaacggaat gccgaaaccc agcgcctcgt agatggtgca gggcgaattg
     8461 tcgaccggcg acggcatcac cgccagtgcc ggccgcgaca gcatgtagcg gatcgcctct
     8521 tgctgtccga ggtcggtcac ggtttgccag cgaaagtgcc agccggcgcc gcggcgcgcg
     8581 agatagtcga ggctgtccgt cccggcaacg ggcgcagact ttcccaggaa ggtgacggcg
     8641 atatcctttg ccgccagcac cccatcgagc cgctccatgg cattgcagaa aacgcgaagg
     8701 cccttgcgct cctcgagccg gccgaagaag acgatctcct tcggatggac gatcgcgtcg
     8761 ctgcgcggcg gactcgcggt gaatttctcg ggatcggcgc ggaagaggtc ggccgtgggt
     8821 atgacgtatt gctggaaata ggttgccgcc ggcagcacga agcctttgct cttcgcccat
     8881 tcgatgatgt agcggctcgg cgcccagagc agatcggccg cgcgtgtgct caagcgctcg
     8941 gcgtagttgt aggccgcgta gaggatgctg tcggggagac gctggttgtg ctcgagcacc
     9001 cattgggacg gactgtgcag cgcgagacaa agcaggctgt cctggaatgc gacaccgagc
     9061 cgcttcatga cgaggcagta gtagccgtcg ccgacgcaat cattgaaatg gaccacgtcg
     9121 aaacggttgc tgcggagatg gtcatagacc aataaaggga cgccgaagcc gcgctgccga
     9181 agcgggccgt cgagccgcgc catgtcggcg aagccgaggg cggtgaagcc gatgccgatg
     9241 ccgcgatagc gctcgcgcca ctcgccgaga tcgacatcgg gcacccacac gccgccggta
     9301 tagaggatac tgaccgcgaa tccggcgccg gccaggctct ccgccaagcc ggtcatcgag
     9361 gtgccgatgc cgccgttttt gaacgggccg acgacttcgc cggagacgat gcacaccgtc
     9421 ggcctgcgct cgctcgccgg cacgctcggc aggccggcga aatccttgta catcagccgg
     9481 tcactgtcca agcgacagca gccggcgaag ccggcgatag agccggtgcg tcaatcgcgc
     9541 gttcgccgcg gcgagttccc gaccgaggcg ttcgatatcc cgatcgcgct gctgaatcgc
     9601 ctcagccagg cgccgcatgt cctggtcgcg ctgctgacct tcctcgctga ggcgctgcat
     9661 gtcccgatcc cgccgctgcg tttcctcgct caggcgctcg atgtcccgat cgcgctgccg
     9721 aagttcctcg tcgaggcgtc gaatctccgt ctcccgctgc acgccgccgc gatccagccg
     9781 gtgaatctcc tgctcctgcc ggatcgctcg cgcgcgcaga tcggcgggat atccggagaa
     9841 tgccgcgccc gacaccggca agccgacgag atgatgcggc agctgccagg cgaccgcggg
     9901 atcgggcgtg tggtggaccg gcgtcacctg gccgcgccag gcgatgccca tgatctgcat
     9961 ggtctggtag accatgacat tgaaaccgcg cgcctcgagc agccgaagcg cggcggcaac
    10021 ctcgggcgat gccaggtcgt ggaggacgaa ggccgcatcg tcagcggcat gcgcgacgca
    10081 agaatcgaca tcgcgttccg gcatgggcgg ctcgtgatcg ccatcgacga cgaacaattc
    10141 ccagaccgca ccgtctttgg ccgcgagcgc ggcgattgcg gcggggctcg ctgcgccatg
    10201 gagcgtgacg gcgtcgcgca cgccgcagca gcggagcgcg tgctcgacgc tgtcgcggtg
    10261 cagcggatcg gaaaggatcg gatcga
//
LOCUS       DASYNS010000418        10820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1042493, whole genome shotgun sequence.
ACCESSION   DASYNS010000418 DASYNS010000000
VERSION     DASYNS010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10820
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042493"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VGP50_12930"
     CDS             <1..184
                     /locus_tag="VGP50_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028330.1"
                     /translation="AREHEWREHDEHERRPDIEHGYRWYAPGYAYNYGYVAPSYVPAP
                     TYSTPVPLFNFGFEFR"
     gene            complement(201..731)
                     /locus_tag="VGP50_12935"
     CDS             complement(201..731)
                     /locus_tag="VGP50_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028331.1"
                     /translation="MADQPPKPPFPMIREAVASFTTRAQLRDAVSRLLAADFSPADIS
                     VLATHDSLEVAGDVPAYRGTPGSSLLAGLTDEVSFLAPLTVAGIVLLTGGPVAVAIAA
                     LVGAGVGTAAIRELLDYYTANRHTSEFARALEAGAVLLWVRVDDPALEASAVRIFEEA
                     GGLNVHIHARPAKAGP"
     gene            889..3489
                     /gene="mutS"
                     /locus_tag="VGP50_12940"
     CDS             889..3489
                     /gene="mutS"
                     /locus_tag="VGP50_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV8028332.1"
                     /translation="MAQYLELKRQHADCLLFYRMGDFYELFFEDAAKAAAALDIQLTK
                     RGRHEGGDIPMCGVPVHAAETYLARLIRQGFRVAVCEQMEEPAEAKKRGSKSAIRRAV
                     VRIVTPGTITEETLLDARRSNYLAALADAGGELALAWLELSTGVFRLQPVPASGLGAV
                     LARLEPGEILLPERLVQRPEFFELLGDWKSALTPLPNARFDSENGRKRLESLFAVRAL
                     DGFGSFARAELAAGGALVDYVSLTQQGKRPLLQPPRRLAAGAVMEIDAATRRNLELTQ
                     TLGGERKGSLLAVIDRTQTGAGARLLAEYLAAPLTDPAAIDERLDAVTFFIADERLRG
                     DMRALLRRCPDIERAMARLALGRGGPRDLAALRDTLGETATLRRFLGERAAAAPPALL
                     AAAERDLGEHGVLVDRLKRALAADLPGIARDGGFIAAGYLAELDELRLLRDESRRMIA
                     NLQARYAGETGIASLKIRHNNVLGYYIEVTPTHAEKLGRDFIHRQSMANAMRYSTVEL
                     SGLESKISSAAERAVALELRLFDDLVAEAGGRAEEIARAAAALARLDVAAALAELAVT
                     ERYCRPVVGDGVGFRVAGGRHPVVEGALRAAGGARFVGNDCALGEGERLWLLTGPNMA
                     GKSTFLRQNALIAILAQAGSYVPAEQAALGVVDRLFSRVGAADDLARGRSTFMVEMVE
                     TAAILNQAGPRALVILDEIGRGTATFDGLSIAWATVEHLHDVNRCRALFATHYHELTS
                     LAAKLPDLACHTMRVKEWQGDVVFLHEVAAGAADRSYGIHVAKLAGLPPAVIARAEAV
                     LAILETGEQSGALARLAEDLPLFSAARAVSPPKRDPLKAALAAIEPDGLTPKAALELL
                     YTLKALAAEG"
     gene            3494..4927
                     /locus_tag="VGP50_12945"
     CDS             3494..4927
                     /locus_tag="VGP50_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010513489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV8028333.1"
                     /translation="MHPFVAQRLGRPGTESGGPTLRRDLGPWGLVALGIGSIIGAGLF
                     ALSGIAAAENAGPAVTLSFLIAALGCAFAGMCYSELATMIPVAGSAYTYAYATMGEMI
                     AWIIGWDLVLEYAVSASAVAVSWTEYLVALLRDLDLEFPARLAQSPFEGSAAGLVNLP
                     AMLVIAALSLLLMRGIRESSAVNTVLVVIKVAIVIIFVALGVGYIDRANYTPFIPANT
                     GRFGEFGWSGVLRGAGVIFFAYIGFDAVSTATQEAKDPQRSVPIGILGSLALCTILYV
                     AFALVLTGMVSYRAMQGDAAPVATAIARTPYRWLRMLIDVGILAGFTSVILVSLLGQS
                     RVFFSMAEDGLLPRVFAAIHPRWRTPWRSNLLFMVFSSVLAGFVPMSLLADMTSIGTL
                     LAFVIVCAGVLVLRRTRPDLPRPYRTPFVPLVPILGIVVCLTLMLALGPENWWRLVVW
                     LVIGLVIYFGYSRRHSHARRAAATETR"
     gene            complement(5036..5959)
                     /gene="rfbD"
                     /locus_tag="VGP50_12950"
     CDS             complement(5036..5959)
                     /gene="rfbD"
                     /locus_tag="VGP50_12950"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV8028334.1"
                     /translation="MNILIVGRRGQLAVELSRANWPLGTRLAIFSREELDVTARGMVE
                     QRVAQAEPDVVINASGYTAVDRAEAEPDAAFAVNCGGVRNLAMAAAKRRISLIHVSTD
                     YVLGGTPGRRPWREDDEPHPAGVYACSKLAGEEAVRESLPTHIILRTSWVFAAHGHNF
                     VRTMLRLGAGRETVRVVDDQIGCPTPAAGLAQVIADMAVACRTGQARFGTYHYAGAGA
                     VSWYDFAKTIFALAGDLVPRPPILQPISTAAFGAQAPRPAYSALDCGKIERDFGIAPA
                     PWLAGLEQVLSELRSEAMRRGKDAEKACARH"
     gene            complement(5993..6640)
                     /gene="rfbC"
                     /locus_tag="VGP50_12955"
     CDS             complement(5993..6640)
                     /gene="rfbC"
                     /locus_tag="VGP50_12955"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015088710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEV8028335.1"
                     /translation="MVISGGGDRRFRPEEQRMQILSSHFRDVKLLRPMRHSDNRGFFS
                     EVYSKRSYAAIDIHNEFVQDNHSLSRKKGVVRGLHFQVPPFSQAKLLRVLRGSVFDVV
                     VDIRAGSPTYGRHIATVLDADDWNQIFIPAGFAHGFCTLEPETEILYKVDNYYSAEHD
                     HGIRWNDPALGIAWPVTGDEAEMSDRDRKLSLLAEEPSVFSVARSDAGRAAVAAA"
     gene            complement(6781..8715)
                     /gene="asnB"
                     /locus_tag="VGP50_12960"
     CDS             complement(6781..8715)
                     /gene="asnB"
                     /locus_tag="VGP50_12960"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV8028336.1"
                     /translation="MCGICGIMLDPSATSADLPRLVLGMTTALAHRGPDGADDWVDPA
                     AGLALGHRRLAIVDLSPGGNQPRISASGRYVITYNGEIYNYRDLRVALAQSGISCHSR
                     SDVEVLVEACAFWGVEATAQRLNGMFAFALWDRQERALTLVRDPLGVKPLYWGRVGSA
                     LLFGSELKALATYPGWAPEIDRDALAAYFRHNYVPAPRTVYRGIFKLEPGHLLVLRRG
                     EAERMSRYWDAESLQGDAASLSDGETTERLDTLLRDAVGAQMMADVPLGAFLSGGIDS
                     STVVALMQAQSTRRVQTFTLGFRAAGYDEAPAARAVAAHLGTEHTELYVGADEARRLI
                     PRIADWFDEPFADSSQIPTYLVAEMTRRHVTVALSGDGGDEVFGGYNRYRLAAGLSRG
                     LRLCPLPLRSAAAAALSALPPAAWDALSRAIPARWRPPQSGDTLHKIAAILTLRDDDA
                     IYRRLVSQWQDPERLVIGAREHESALWNGTLAKRVPDFLARMQYLDLVTYLPDDILTK
                     VDRTSMAVSLEARVPLLDPRVVAFGARLRPRQKIRRGRGKWLLRQVLYRYVPPSLVER
                     PKTGFGIPLDEWLRGPLRGWAEDLLSEARLRSDGILDPPVIRMAWQDHLSGRRNTHYP
                     LWCVLMFQEWQSRWCGARGA"
     gene            8932..9873
                     /locus_tag="VGP50_12965"
     CDS             8932..9873
                     /locus_tag="VGP50_12965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV8028337.1"
                     /translation="MRQYAPALSKADIDVSEYWTGLGSYPPRSPWLRPAWLAGTLAQR
                     VPQVAAGWGADVTWFQREMVSTLVTFEGFTRRPRLLDIDDAVHLFRDGRTARRLARLA
                     DLVVVGNEWLAEVWRRWAPAIEILPTAVATDFAPAPLPERPVIGWIGTASNLRYLTKL
                     APALAEALRRFPHATLAVCCDQAPDLQGLPVTHVAWAPEIERPFLDSLTIGLMPLDDT
                     LWERGKCSYKMLQYMAAARPSVVSPVGMNATILAQGDVGLAATSFEEWVAALSRLLAE
                     PATAEQMGRAGRALALSRYSIAALAPRLAGLLHRLAK"
     gene            9962..>10820
                     /locus_tag="VGP50_12970"
     CDS             9962..>10820
                     /locus_tag="VGP50_12970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV8028338.1"
                     /translation="MRVLHVITDLDIGGTEIMLWQLVAAGVCNASDSTVVSLTDDGPI
                     GARLRSLGVSVCNLGMRRRAGDLLRLRTLAPLVRRMRPQLIQGWMYHGNLAASVAGVC
                     DTGGAPVLWSVRQSLHEIAAERRTTKVAIRLGALLSRHAVSIIYNSETAARQHEALGY
                     HGRRSVVIPNGFDCDRFRPSADARRRIRAELGIAEDAVCIGLVGRLHPVKDHDSFLRA
                     ASVIARRHQQACFVMAGLGVTAQEPSLTGLISAAGLAGRVRLLGVRADMPAVNAALDV
                     ACSSSRNEGF"
BASE COUNT         1626 a         3750 c         3651 g         1793 t
ORIGIN      
        1 tgcccgcgag cacgaatggc gcgagcatga cgagcacgag cgacgccccg acatcgagca
       61 cggctatcgc tggtacgccc ctggctacgc ctacaattac ggctatgtcg cgccgagcta
      121 tgtgccggct ccgacctaca gcacgccggt gccattgttc aacttcggct tcgagttccg
      181 ctgaccccag cgcctgcagc tcaggggccg gctttggccg ggcgggcgtg gatatggacg
      241 ttgagtccgc ccgcctcttc gaagatccgc acggccgatg cctcgagcgc cgggtcgtcg
      301 acccggaccc agagcagcac cgctcccgcc tcgagggcgc gggcgaattc gctggtgtgc
      361 cggttggcgg tgtagtagtc gaggagctcc ctgatggccg ccgtgccgac gccagcgccg
      421 acaagcgccg cgatcgcaac cgcgaccggc ccgccggtca gcagaacgat ccccgccacc
      481 gtcagcggcg ccaggaagct tacctcgtcg gtaagcccgg caagcagcga tgacccgggt
      541 gtcccgcgat aggccgggac gtcgcccgcc acttcaagcg aatcatgggt ggcaagcacg
      601 gagatgtccg ccgggctgaa atctgccgcc aggagtcgcg acacggcgtc gcggagctgc
      661 gcccgcgtcg tgaaggatgc gaccgcttca cgaatcatcg gaaagggcgg cttcggcggc
      721 tggtcggcca tggctcgctc gtttctcctc tgttccagcc gcttcgccag cgtgctagcc
      781 tccgccgcca tcctcggcag cgggcccatg aacgatagcg tattgcatct cccgcgcgat
      841 agcgccgccc ctccatcatc gcccgccgcc gacgcggcga cgccggccat ggcgcaatat
      901 ctcgagttga agcgccagca tgccgattgc ctgctgtttt accgcatggg cgacttctac
      961 gagctcttct tcgaggacgc ggcgaaggcg gccgcggcgc tcgacattca gctcaccaag
     1021 cgcggccggc acgagggcgg ggacatcccg atgtgcggcg tcccggtcca tgccgccgag
     1081 acctatctcg cccgcctcat ccgccagggg tttcgcgtgg ccgtctgcga gcagatggag
     1141 gagccggccg aggccaagaa gcgcggcagc aagtcggcga tccgccgcgc cgtggtgcgt
     1201 atcgtcacgc ccggcaccat caccgaggag acgctgctcg atgcccggcg cagcaattac
     1261 ctggcggcgc tcgccgatgc cggcggcgag ctggcgctgg cctggctcga gctttcgacc
     1321 ggcgtctttc gtctccagcc ggtaccggcc tcgggcctcg gcgcggtgct ggcgcggctc
     1381 gagccgggcg agatcctctt gcccgagcgc ctcgtgcagc gcccggaatt cttcgagctg
     1441 ctgggcgact ggaagagcgc gctgacgccg cttcccaatg cccgcttcga cagcgagaac
     1501 gggaggaagc gcctcgaatc gctcttcgcc gtgcgcgccc tcgacggctt cggcagcttt
     1561 gcccgcgccg agctcgcggc cggcggcgcg ctcgtcgact atgtgagcct gacgcagcag
     1621 ggcaagcggc cgctgctcca gccgccgcgc cgccttgccg ccggcgcggt gatggagatc
     1681 gacgccgcga cgcggcgcaa tctcgagctc acgcagaccc tgggcggcga gcgcaagggc
     1741 agtctgctcg cggtcatcga ccgcacccag acgggcgccg gcgcccggct cctcgccgaa
     1801 tacctggcgg cgccgctcac cgatcccgcc gccatcgacg agcggctcga cgccgtcacc
     1861 ttcttcatcg ccgacgagcg gctgcgcggc gacatgcgcg cgttgctcag gcgctgtccc
     1921 gatatcgagc gcgccatggc gcgcctcgcg ctggggcgcg ggggcccgcg cgatctggcg
     1981 gcgctgcgcg acacgctggg cgagacggcg acgctccgac gcttcctcgg cgagcgcgcg
     2041 gccgccgcgc cgccggcgct cctggccgcg gccgagcgcg atctcggcga gcatggcgtc
     2101 ctggtcgacc ggctgaagcg ggcgctcgcg gccgatctgc cggggatcgc gcgcgacggc
     2161 ggcttcatcg ccgcgggtta tctcgccgag ctcgacgagc tgcgcctgct gcgcgacgag
     2221 agccgtcgca tgatcgccaa tctgcaggcg cgctatgccg gcgagaccgg catcgcctcg
     2281 ctcaagattc gccacaacaa cgtgctcggc tattacatcg aggtgacgcc gacccatgcc
     2341 gagaagctcg gccgggattt catccatcgc cagagcatgg ccaatgccat gcgctattcg
     2401 acggtggagc tgagcggcct cgagagcaag atctcttcgg cggccgagcg cgcggtcgcg
     2461 ctggagctcc ggctgttcga cgatctggtc gcggaggcgg gcgggcgggc cgaggagatc
     2521 gcgcgggcgg cggcggcgct ggcgcggctc gacgtggcgg cggcgctcgc cgagctggcg
     2581 gtgaccgagc gctattgccg cccggtggtc ggcgacggcg tgggattccg ggtggcgggc
     2641 gggcggcatc cggtggtgga gggggcgctc cgggcggcag gcggtgcgcg tttcgtgggc
     2701 aacgactgcg ctttgggcga gggcgagcgg ctctggctgc tcaccggtcc caacatggcg
     2761 ggcaagagca ccttcctgcg ccagaacgcg ctcatcgcca tcctggcgca ggccggctcc
     2821 tatgtgccgg ccgagcaggc ggcgctcggc gtcgtcgatc gcctcttcag ccgggtcggc
     2881 gcggccgacg acctggcgcg gggccgctcg accttcatgg tcgagatggt cgagacggcg
     2941 gcgatcctca accaggccgg cccccgcgcc ctcgtcattc tcgacgagat cggccgcggc
     3001 acggccacct tcgacggcct ctcgatcgcc tgggcgacgg tcgagcacct ccatgacgtg
     3061 aaccgctgcc gcgcgctctt cgccacgcat tatcatgagc tgacatcgct cgcggcgaaa
     3121 ctccccgacc tcgcctgcca caccatgcgc gtcaaggaat ggcagggcga cgtcgtgttc
     3181 ctccacgagg tggcggcggg cgccgccgac cgctcttatg gcatccatgt ggcgaagctc
     3241 gccggcctcc cgccggcggt catcgcgcgc gccgaggcgg tgctggcgat cctcgagacg
     3301 ggcgagcaga gcggcgctct ggcgcggctc gccgaggacc tgccgctctt cagcgcggcg
     3361 cgcgccgtgt caccgccgaa gcgcgatccg cttaaagcgg cgctcgccgc catcgagccg
     3421 gacgggctca cgcccaaagc ggcgctcgag ctcctctaca cgctgaaggc gctggccgcg
     3481 gaaggctgag cccgtgcacc ccttcgtcgc gcagcggctc ggccgcccgg gaacggagag
     3541 cggcgggccg acgctgcgcc gtgatctcgg gccttggggc ctcgtcgcgc tcggcatcgg
     3601 ctcgatcatc ggcgccggcc tcttcgcgct ctcgggcatc gcggcggcgg agaatgccgg
     3661 cccggcggtg acgctctcct tcctcatcgc ggcgctcggc tgcgccttcg ccggcatgtg
     3721 ctacagcgag cttgcgacca tgatcccggt cgcgggcagc gcctacactt atgcttatgc
     3781 gacgatgggc gagatgatcg cctggatcat cggctgggac ctcgtcctcg aatatgccgt
     3841 gagcgcgtcg gcggtcgcgg tgagctggac cgagtatctc gtggcactgc tgcgcgacct
     3901 cgatctcgag ttccctgcgc gcctggcgca gagcccgttc gagggcagcg ccgccggcct
     3961 cgtcaatctg ccggcgatgc tcgtcatcgc cgcgctctcg ctgctcttga tgcgcggcat
     4021 ccgcgaatca tcggcggtca acacggtgct cgtcgtcatc aaggtggcga tcgtcatcat
     4081 cttcgtggcg ctcggcgtgg gctacatcga tcgcgccaac tacacgccgt tcatccccgc
     4141 caataccggc cgcttcggcg aattcggctg gagcggagtc ttgcgcggcg ccggcgtgat
     4201 cttcttcgcc tatatcggct tcgacgccgt gtcgacggcg acgcaggagg cgaaggatcc
     4261 gcagcgcagc gtgccgatcg gcatcctcgg ctcgctcgcc ctctgcacca tcctctatgt
     4321 cgccttcgcg ctggtgctga ccggcatggt gagctaccgc gcgatgcagg gcgacgcggc
     4381 gcccgtcgcg accgcgatcg cgcgcacgcc ctatcgctgg ctcaggatgc tgatcgatgt
     4441 cggcatcctc gccggcttca cgtcggtgat cctggtgtcg ctgctcggcc agtcgcgggt
     4501 cttcttctcg atggccgagg acggattgct gccgcgcgtc ttcgcggcga tccatccgcg
     4561 ctggcgcacg ccctggcgct ccaatctgct gttcatggtg ttttcgagcg tgctcgccgg
     4621 cttcgtgccg atgtcgctcc tcgccgacat gacgagcatc ggcaccctcc tcgccttcgt
     4681 catcgtctgc gccggcgttc tcgtgctccg ccgcacgcgg cccgatttgc cccgccccta
     4741 ccgcacgccc ttcgtgccgc tggtgccgat cctcggcatc gtcgtgtgct tgacgctgat
     4801 gctggcgctc gggcccgaga actggtggcg cctcgtcgtc tggctggtca tcggcctcgt
     4861 catctatttc ggctacagcc ggcggcacag ccatgcccgc cgcgccgccg cgacggagac
     4921 acgctgaaac aacacccaca aggcggcgac gcggcgcagc cccgtcaacg ccggcaagat
     4981 ccgcccccgc tgctcggagc aggcagtgga aagtcggcga gaagggtggt gattctcaat
     5041 ggcgcgcgca tgctttctcg gcgtctttgc cgcgacgcat tgcttcggat cggagctcgc
     5101 tcaacacctg ctccaggccg gcgagccatg gcgcgggcgc gatgccgaag tcgcgctcga
     5161 tcttgccgca atcgagcgcg gaataggccg gccggggcgc ctgcgcgccg aacgccgcgg
     5221 tggagatcgg ctgcaggatc ggcggccgcg gcacgaggtc gccggcaagc gcgaagatgg
     5281 tcttggcgaa atcgtaccag ctgaccgcgc ccgcgccggc ataatggtag gttccgaacc
     5341 gtgcttgtcc cgtccggcaa gcgaccgcca tatcggcgat gacctgtgcg agaccggcgg
     5401 ccggtgtcgg acagccgatc tggtcatcga ccacgcgcac ggtctcgcgt cccgcgccaa
     5461 gacggagcat cgtgcggacg aagttatgac catgcgcggc gaagacccag gaggtgcgca
     5521 ggatgatgtg cgtcgggagg ctctctctga ccgcctcttc cccggcaagc ttcgagcatg
     5581 catagacgcc ggccgggtgc ggctcatcat cttctcgcca gggccgccgc ccgggcgttc
     5641 cgccgaggac gtaatccgtc gagacgtgga tgagcgagat gcggcgcttc gccgccgcca
     5701 tggcgagatt tcgcacgccg ccgcagttga cggcgaaggc tgcatcgggc tccgcctccg
     5761 cgcggtcgac cgccgtgtag cccgatgcat tgatcacgac gtcgggctcg gcctgcgcga
     5821 cacgttgctc caccatgccg cgagccgtga cgtcgagctc ctccctgctg aagatcgcaa
     5881 gccgcgtgcc caagggccaa ttggcacgcg acagctcgac agcgagctgc ccccggcgtc
     5941 cgacgatgag aatgttcatg tcgggcgacg gcgagcgtgc gggtctctga actcaggcgg
     6001 ccgcaacggc agcgcgcccg gcgtcgcttc gggcgacgga aaagacgctg ggctcctcgg
     6061 cgagaagcga cagcttgcgg tcccggtcag acatctcggc ctcgtctccg gtgacgggcc
     6121 atgcgatacc gagcgcggga tcgttccagc ggatcccgtg atcgtgctcg gccgaatagt
     6181 agttgtccac cttgtagaga atctctgttt ctggctcgag cgtgcagaag ccgtgcgcaa
     6241 atccggccgg tatgaaaatc tgattccagt cgtcggcgtc gaggacggtg gcgatgtgac
     6301 ggccatatgt cggagatccc gcgcggatat cgacgaccac gtcaaacacc gagccgcgca
     6361 gaacgcgtag cagcttggct tgagagaagg gcggcacttg aaaatgcaac ccgcgcacga
     6421 cgcccttttt tcgcgacaga gaatgattgt cctggacgaa ttcgttatgg atgtcgatcg
     6481 ccgcataaga ccgtttgctg tacacctcgc tgaagaagcc gcggttgtcg ctgtggcgca
     6541 tcggacgaag cagtttgacg tcgcggaaat gggagctcag gatctgcata cgttgctcct
     6601 cagggcggaa acgcctgtcg ccgccgcccg aaatcaccat caccatacgc cataaggcgg
     6661 ccgcaagagc gagtttgtcg ctgctggctg cgggttttgc gtcgtttctt tcgtgcggca
     6721 cgatacattg ggcacaatac gcagtgacgg gacacgcgcg cgcgtggcaa ggccgccagg
     6781 ctaggcaccg cgcgcaccgc accagcggct ctgccattct tgaaacatca agacgcacca
     6841 gagcgggtag tgcgtgttgc gccgtcctga gagatggtcc tgccaggcca tccgtatgac
     6901 gggcggatcg aggatcccat ccgagcgcag ccgcgcttcg ctcaagagat cctcggccca
     6961 gccgcgcagc ggcccgcgca gccattcatc gagcggaatg ccgaacccgg tcttcggacg
     7021 ctccaccagc gacggcggaa cgtatcggta aagcacttgg cgcagcagcc atttgccgcg
     7081 tccccgacga attttctggc gcggccgcaa tcgcgcgccg aaggccacga cgcgcggatc
     7141 gagcagcggt acccgcgcct cgagggagac cgccatgctc gtgcgatcga ccttggtcag
     7201 gatgtcgtcg ggcaaataag tgacgagatc gagatactgc atgcgcgcga gaaagtcggg
     7261 cacccttttc gccagcgtgc cgttccacag cgcgctctcg tgctcgcgcg cgccgatgac
     7321 caggcgctcc ggatcctgcc attgcgatac caggcgccgg tagatcgcgt cgtcgtcgcg
     7381 aagcgtcaga atcgcggcga tcttgtgcag cgtgtcgccg ctctgcggcg gtcgccatcg
     7441 cgccggtatc gctcgcgaga gcgcgtccca cgcggccggc ggcaatgccg agagcgccgc
     7501 cgccgcggcg ctgcggagcg gcaggggaca gaggcgcaag ccccgtgaca gaccggcggc
     7561 gagccgatac cggttgtagc cgccgaagac ctcgtcgccg ccgtcgccgg acaaggcgac
     7621 ggtgacatgg cggcgcgtca tctcggcgac gagataggtc gggatctgcg acgagtcggc
     7681 gaacggctca tcgaaccaat cggcgatgcg cggaatgagg cggcgcgctt cgtcggcgcc
     7741 gacataaagc tcggtgtgct cggtgccgag atgggcggcg acggcccgcg ccgcgggcgc
     7801 ctcgtcataa cccgccgcgc ggaaacccag ggtgaacgtc tggacgcgtc gcgtgctctg
     7861 cgcctgcatc aacgccacca ccgtcgatga atcgatgccg cccgacagaa atgcgccgag
     7921 cggcacatct gccatcatct gcgcgcccac ggcgtcgcgc agcagtgtgt cgagccgttc
     7981 ggtcgtctcg ccgtcgctca ggctcgcggc atcgccctgg agcgattcgg catcccagta
     8041 gcggctcatg cgctccgcct cgccgcggcg gagcacgagg agatgtccgg gctcgagctt
     8101 gaagatgccg cgatagacgg tgcgcggtgc cggcacgtag ttgtggcgaa aatacgcggc
     8161 cagcgcatcg cgatcgattt ccggcgccca gccgggatat gtcgccagcg ccttcagctc
     8221 cgagccgaag agcagagcgc tgccgacccg gccccaataa agcggcttca cccccaacgg
     8281 gtcgcgcacc aaagtgagcg cgcgctcttg ccgatcccag agggcaaaag cgaacatgcc
     8341 gttgaggcgc tgcgccgtcg cctcgacacc ccagaaggcg caggcttcga cgagaacctc
     8401 cacatccgac cggctgtggc aggagatgcc gctctgcgcg agcgccacgc gcaaatcgcg
     8461 atagttgtag atctcgccat tgtaggtgat gacatagcgc ccgctcgccg agatgcgcgg
     8521 ctgattgccg ccgggcgaga gatcgacgat cgccagcctg cgatgtccga gcgcgaggcc
     8581 ggcggccgga tcgacccaat cgtcggcgcc atcgggcccg cgatgagcga gggccgtcgt
     8641 catgccgagg accagcctcg gcaagtcggc gctcgttgcg gaagggtcca gcatgattcc
     8701 gcaaatgccg cacaccagcc ggccccgcca tcagcgccgc ccgaacacag gaagcagccg
     8761 cttaacgata ccacatcgac gatcgagctc atggcgggtg agcaatcact tgacgcgccg
     8821 tcctggtacg ccttgattgg ccgccgcggg agcacgtcct tggcacttcg ggtctcactt
     8881 cgtgtcgccg cattcacagc gtctgccgtc gcgccggcgt cgcgattccg ggtgcggcag
     8941 tatgcgccgg cgctgagtaa ggccgatatc gatgtcagcg agtactggac cggtctcggt
     9001 tcctacccgc cccgcagccc ttggctgcgg ccggcctggc tcgccggcac actggcgcag
     9061 cgcgtgccgc aggtagcggc cgggtggggc gccgatgtca cttggtttca gcgagagatg
     9121 gtctcgacgc tcgtcacctt cgaagggttc acgcggcgcc cgcggcttct cgacatcgac
     9181 gatgccgtgc atctcttccg cgacggacgc acggcacggc gcctggcgcg gctcgccgac
     9241 ctcgtcgtgg tgggaaacga gtggctcgcc gaggtgtggc gtcgctgggc gccggccatc
     9301 gagatcttgc cgaccgccgt cgccactgat ttcgcgccgg cacctctccc cgagcgtccc
     9361 gtcatcggct ggatcggcac ggcctcgaat ctgcgctacc tgacgaagct ggctcccgcg
     9421 ctcgccgaag cgctgcgacg ctttccccat gccacgctcg ccgtctgttg cgaccaggcc
     9481 ccggacttgc aaggcctgcc cgtcactcac gttgcctggg cgccggagat tgagcggcca
     9541 tttctcgatt cgctgacgat cggcctcatg ccgctcgacg acaccctctg ggagcgcggc
     9601 aaatgcagct acaagatgct gcaatacatg gccgccgcgc ggccttcggt ggtctctccc
     9661 gtgggcatga acgcgacgat cctggcgcag ggcgacgtcg gcctcgcggc gacgtccttt
     9721 gaagaatggg tggcggcgct gtcgcggctc ttggctgagc ccgcgaccgc ggaacagatg
     9781 gggcgggccg gccgcgcctt ggcgctgtct cgctacagca ttgccgcgct cgctccccgc
     9841 ctcgccgggc tcctgcaccg gctcgctaaa tgattgtggt tgactaacaa aatacaactc
     9901 tgtcattgtc gggcagcgaa ctcaccttcg aggtgccatc gcctgcttcg gtcttgcgcc
     9961 aatgcgcgtg ttgcatgtca tcacggacct cgatatcggc ggcacggaga tcatgttgtg
    10021 gcaactggtc gccgcgggcg tatgcaatgc ttccgattcc accgtggtgt cgttgacgga
    10081 tgacggcccc atcggcgccc ggctgagatc gcttggcgtg tcggtttgca acctcggcat
    10141 gcggcggcgt gccggggatc tgctgcggct tcgcacgctg gcgccgctcg tgcggcggat
    10201 gcggccgcaa ctcattcaag gctggatgta tcacggcaat ctcgctgcca gcgtcgccgg
    10261 cgtctgcgac accggcgggg cgccggtcct ttggagcgtg cggcaatcgc tgcacgagat
    10321 cgccgccgaa cgacgcacga caaaggtggc aatccgcctt ggcgcgctgc tgtctcgtca
    10381 tgccgtctcg atcatctaca acagcgagac cgccgcccgg cagcatgagg ccttgggcta
    10441 tcatggccgt cgcagcgtcg tcattccgaa cggtttcgac tgcgatcgtt tccggccgtc
    10501 tgcggacgcg cggcgccgca tccgcgccga gctcggcatc gccgaggatg ccgtgtgcat
    10561 cggcctcgtc ggccgccttc atccggtcaa ggatcatgat tcctttctcc gcgccgcgag
    10621 cgtgatcgcg cgacgccacc agcaagcctg ttttgtcatg gcgggactgg gcgtcaccgc
    10681 ccaggaaccg tcgctgaccg gccttatcag cgccgcggga ttggcaggtc gcgtccgtct
    10741 tcttggcgtg cgcgccgata tgccggcggt caacgccgct ctcgatgtcg cctgctcgag
    10801 ctccaggaac gagggcttcg
//
LOCUS       DASYNS010000419        18977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_106860, whole genome shotgun sequence.
ACCESSION   DASYNS010000419 DASYNS010000000
VERSION     DASYNS010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 18977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18977
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106860"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VGP50_12975"
     CDS             complement(<1..274)
                     /locus_tag="VGP50_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEV8028339.1"
                     /translation="MTALRKRRHPSLLAAREGGAAIEFAMIMPIAVAMLVLMFDLGTG
                     GYMKARVLSAAQAGTTYVEAHGWDRRGIVLAVRAATNLPSVNIQATL"
     gene            complement(271..720)
                     /locus_tag="VGP50_12980"
     CDS             complement(271..720)
                     /locus_tag="VGP50_12980"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEV8028340.1"
                     /translation="MLGKLIRNLSRDRGGAVAVMFALSATVLLGFVGLGAEVALWYID
                     KRAAQGAADTAAYSALIAYHAGDTADFVATARAVAAQYGFIDAAGGVSVTVNQPPQSG
                     SHSSDPTAIEVVISAPEIPLFSKMFLSAPGVAARAVAASAAGAKLVE"
     gene            complement(805..1284)
                     /locus_tag="VGP50_12985"
     CDS             complement(805..1284)
                     /locus_tag="VGP50_12985"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8028341.1"
                     /translation="MQRKRFSDMPCPIARALDIVGERWTLLILRDISCGIRRFDALQE
                     RLGIARNVLTQRLAWLVEQGLLEKRAYEARPPRYEYRPTEKGRGLFPVLVTLMAWGER
                     WSPPDGVTIAVVDKDTGAALEPVLVDARTGRPLEPRAMRLQTAVVPRSRRNRAMRSG"
     gene            1344..1817
                     /locus_tag="VGP50_12990"
     CDS             1344..1817
                     /locus_tag="VGP50_12990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8028342.1"
                     /translation="MTECQRILVPRWGEILIRRLWLPGDGAAYHRFGAALAPDDLRLR
                     FAVPTHWGPATAQRLAPLDGTAFAAFDRGGEILGIGDVVEGEVALAIRSDLKRRGLGR
                     ALLERIREHALERGMTELVGCVLAENRPMLALAQIAGFRAAGFEGSMVSLRLCLP"
     gene            1847..2563
                     /locus_tag="VGP50_12995"
     CDS             1847..2563
                     /locus_tag="VGP50_12995"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8028343.1"
                     /translation="MMRKAVIIGVGPDQGLGAQLCKRFAGEGLHVFVAGRTKSALDAV
                     VDDIASAGGRATPVVADATKEADVVALFNQAGGDLDLAIYNAGNNTPGRIVEMEAEYF
                     ERSWRVVCFGGFLFGREALRRMVPAGGSLLFTGASASLRGRAGYGAFNSAKAGLRALA
                     QAMAKEYAKDGVHVGHVVVDGAIGGEKIRKRFPEMVNLVGEDGLIGIDGIVDAFVFLH
                     KQPRAAWTFELDLRTSKESW"
     gene            complement(2695..4116)
                     /gene="pyk"
                     /locus_tag="VGP50_13000"
     CDS             complement(2695..4116)
                     /gene="pyk"
                     /locus_tag="VGP50_13000"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV8028344.1"
                     /translation="MRRRRSAKIVATLGPASSEPGTIEALFRAGVDVFRLNFSHGAHE
                     QHRARLDAIRALEKETGRPISILADLQGPKLRLGTFAQGPVRLVGGGHFRLDLDPTPG
                     TLERAPLPHPEIFAAVEPGTELLLNDGNVRLRVESCGADFAETTVVFGGELSDRKGVN
                     VPNAVLPLSALTPKDRTDLAYALELGVDWIALSFVQRPEDVAEAKKLITGRAGVMVKL
                     EKPAGVRRLAEIVELTDAVMVARGDLGVEMPPEDVPPVQKQIIHACRVAGKPVVVATQ
                     MLESMVHAPVPTRAEASDVATAIYEGADAVMLSAETAAGEYPIEAVTMMDRIIRRVQA
                     DPLYDANLHAFTQAEPERTTSDAISAAARQVAYTVSAAAIVSYTTSGATALRTARERP
                     GVPILALTSNLQTARRLALVWGLHCVHTSDVTNFGDMVKKASQITREEGFADTGERIV
                     VTAGVPFGTPGATNTLRIAWIAD"
     gene            complement(4131..5423)
                     /locus_tag="VGP50_13005"
     CDS             complement(4131..5423)
                     /locus_tag="VGP50_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV8028345.1"
                     /translation="MDPPRHDNAPRFLRALFDAALAAADPARTLPAHLPPPPRGRTVV
                     LGAGKAAAAMAKAVEEHWQGPLSGIVITRYGHGLPCRDIDVVEAGHPVPDSAGSAAAA
                     RLLASVAGLAADDLVLFLASGGGSALMALPATGITLEDKRAVTRALLRSGATIGEINC
                     VRKHLSAIKGGRLAAAAAPAPVVTLAISDVPGDDPAVIASGPTVADPTTFADARAILA
                     KYAIAPPPSVAAHLAAAHDETPKPGDPRLARSRFVLIATPQEALAAAAAASRAAGVTP
                     IVLGDAIEGEAHQVALVHAAIARQAASRHLLVGDRMLPLPAVLLSGGETTVTVTGRGR
                     GGRNAEFLLALAVALDGHPAIHAIACDSDGIDGTEDNAGAVAGPDTVARAATLGLDLR
                     ARLADNDGYGAFHALGDLVVTGPTRTNVNDFRAILVLA"
     gene            5577..6356
                     /locus_tag="VGP50_13010"
     CDS             5577..6356
                     /locus_tag="VGP50_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEV8028346.1"
                     /translation="MLLAADDPPPVECCNDHGRAPVLLTCDHASAAVPRALNGLGVPP
                     VAIREHIGWDIGAAAVTRLLAMALDAPALLSGYSRLVVDCNRDPDDRTSIPAESDGVA
                     VPGNRALSPEARIRRLEACFAPYHMAIAARLDAFAAAGVAPAFLSVHSFTPVMKGMAR
                     PWHVGILWDKDPRIPKPLLAALGADPSITVGDNEPYSAREPAGFTVRYHAIARGLPHV
                     ALELRQDLIAEAGGAAAWAARLAGVLRPILAQRAIYRVERY"
     gene            complement(6363..6590)
                     /locus_tag="VGP50_13015"
     CDS             complement(6363..6590)
                     /locus_tag="VGP50_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028347.1"
                     /translation="MKTIKNTPPHALWQFPRVWAALLPAPLLGIAWIAGDLLKHRSPE
                     YYVVDSGVLLAILLLTVGGYALVRRSSRHAR"
     gene            6718..7002
                     /locus_tag="VGP50_13020"
     CDS             6718..7002
                     /locus_tag="VGP50_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEV8028348.1"
                     /translation="MDDKTRIELEAGAFRRLVQHLRERTDVQNLALMNLAGFCRNCLA
                     RWYREEAESRGIAVADPEARKIVYGMPYDEWKAKYQKDEKKPASSGGHQH"
     gene            7087..7359
                     /locus_tag="VGP50_13025"
     CDS             7087..7359
                     /locus_tag="VGP50_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015989315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEV8028349.1"
                     /translation="MPDVGGIAGERLRSFIERIERLEEEKRALAQDIKEVYAEAKGTG
                     FDVKIMRQIVKIRKMDQNELDEQETLLDVYKRALGMTVAFAEEAAE"
     gene            complement(7366..7761)
                     /locus_tag="VGP50_13030"
     CDS             complement(7366..7761)
                     /locus_tag="VGP50_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV8028350.1"
                     /translation="MSTETNKQLLRHIYEELSKGNVQTLLDSLAEDVRWTIIGTTALS
                     GTYCGKQEIIDKLLKPLRVRLAGPIVFTQDRLIAEGDAVVMQARGRATSRSGKPYNNT
                     YCIVARIAGGKVTEMTDYADTELVTMALG"
     gene            7854..8336
                     /locus_tag="VGP50_13035"
     CDS             7854..8336
                     /locus_tag="VGP50_13035"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8028351.1"
                     /translation="MQPSRFLYDLENCSIRRVLDLLGEKWTLLVLREAVYGVRRFDDF
                     ARALGCGRGVLSDRLRTLTDADILARVEYQEPNQRARAEYHLTDKGRDLFPALLALSQ
                     WGDRWMPPAGGPVARVTHRKSGRPVAVVMTGDPKVEALTMRDVEIAPGPGAKRIGRAR
                     "
     gene            complement(8450..11914)
                     /locus_tag="VGP50_13040"
     CDS             complement(8450..11914)
                     /locus_tag="VGP50_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEV8028352.1"
                     /translation="MALAAVSLDDKYTLEQGRIYLSGIQALVRLPMMQRRLDRAAGLD
                     TAGFISGYRGSPLGGLDQNLWQAQRFLEQHQIRFQPGINEDLAATAVWGSQQTSMFPG
                     AKYDGVFAMWYGKGPGVDRSGDVFKHANAAGTSRHGGVLVLAGDDHAAKSSTLAHQSE
                     YAFIDASIPVLNPAGIQDILDFGLFGWALSRFSGLWVGLKTITETVESSASVFVDPAR
                     VQIALPEDFALPPGGLNIRWPDHNALEMERRLRDGKLPAVLAFARTNRLDRVLIDGPR
                     PRLGIITTGKSYLDLRQALSDLGIDDAQAAAMGLRLYKVALTWPLEPEGVRRFAQGLE
                     EILVVEEKRPVVEQQVKDILYNRSERPRILGKEDEHGAPLLRSTGELDPVQVARVLAE
                     RIQRFHPTPRLTERLNFLTALQRQQQGKPAIERVPYFCSGCPHNTSTRVPEGSRATGG
                     VGCHYMAIWMDRDTATVTQMGGEGATWIGQAPFTETPHVFANLGDGTYFHSGILAIRA
                     ALAAGVNITYKLLYNDAVAMTGGQPIEGQLTVPQITRQLAAEGVTRIVVVTDEPDKYP
                     VNAGFAPDVAVFHRERLDAVQRDLRETTGVSVLVYDQTCAAEKRRRRKRGTFPDPQKR
                     VFINDLVCEGCGDCSRTSNCLSVVPLETEFGRKRAIDQSSCNKDFSCVEGFCPSFVTV
                     HGGTLRRHHAPQGQDEIPALPEPSLPALAEPYDILVTGVGGTGVVTIGALLGMAAHLE
                     GKGCSILDQTGLSQKGGAVMSHIRLAGAPDDLHAARIALGRARLLLGCDIVVAATPDS
                     LMRLGGRDARAVVNEHEIITGDFTRNPDFAIPGRDLAQKIAGAVGRENADFLDATRLA
                     TALLGDAIATNLFLLGFAYQKGFVPVSATAIERAIALNAVAVDFNHRAFRYGRWAALD
                     REAVERIATPEAAADERLSESVAEMVARRVAFLTDYQDAAYAERYRRLVRRAEAAEEL
                     RAPGHQGLAEAVARNFFKLMAYKDEYEVARLYTDGSFEKKLAQQFDGDFSLEFHLAPP
                     LLAERDPLTGHQKKRRYGAWMMTAFRLLAKLKFLRFTPLDPFGRSEERRVERRLVAEY
                     EALVDELIPGLTPENHGIAVELARIPQQIRGFGHVKAANLADARARQEKLLAAFRSPP
                     APHAIAAE"
     gene            12232..13599
                     /locus_tag="VGP50_13045"
     CDS             12232..13599
                     /locus_tag="VGP50_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV8028353.1"
                     /translation="MSDAVILGLKERFGERVSTAAAVREQHGKDESYHAPRQPDAVVF
                     ARSTDDVVDIVKLCAAHQTPVIPFGTGTSLEGHVAALEGGVCVDLSAMNRVLRVSAAD
                     LDCTVEAGVTRKQLNTHLRDTGLFFPIDPGADASLGGMAATRASGTNAVRYGTMRENV
                     LALTVVLADGRVLRTARRARKSAAGYDLTRLFIGSEGTLGVITEVTLRLYGIPEAIMA
                     AVCPFPSVEAAVDTVVATIQAGVPIARIELLDAKQMEAVNKYAKLDHAVAPTLFFEFH
                     GTTRGVEEQVAQVKAFAAEQGGNDFHWAATAEERSKLWQARHDAYYAALALRPGAKGW
                     ATDVCVPISRLAECIAETRRDVDQSFVTAVLVGHVGDGNFHLIFVLDPTKPEEFAEAS
                     RINDRMVARAIAMDGTCTGEHGIGYGKMDFLIAEHGEGVAVMRSLKHALDPDNIMNPG
                     KIVRM"
     gene            complement(13717..14229)
                     /locus_tag="VGP50_13050"
     CDS             complement(13717..14229)
                     /locus_tag="VGP50_13050"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEV8028354.1"
                     /translation="MSRAFVSESDTDAAADMLPELPQSTHPNYVTGRGLRLLEERLAG
                     AQQQQRRLSARPDDPAKDLEAARTAREIRYLEARIERAIPVDPAQAPADEVAFGAVVR
                     VVDAAGERQEFAIVGEDEADAEHGKVSWVSPLARALIGSGVGDVVLWRRPAGEVELEI
                     LSIRYPERAS"
     gene            14310..14828
                     /locus_tag="VGP50_13055"
     CDS             14310..14828
                     /locus_tag="VGP50_13055"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEV8028355.1"
                     /translation="MAPDPRIALATGDITKLAVDAIVNAANSSLLGGGGVDGAIHDAA
                     GPGLQAECLTLGGCPTGEARITSGHRLRARFVIHTVGPVWRGGRADEWELLARCYRSS
                     FALASSHAIRSIAFPAISTGAYRFPALQACAIAVHETHHFLARNDTLEKVIFVAFDAE
                     IEELYRRALGLL"
     gene            complement(14825..14998)
                     /locus_tag="VGP50_13060"
     CDS             complement(14825..14998)
                     /locus_tag="VGP50_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HEV8028356.1"
                     /translation="MAEIVNLNRYRKQRLRKEAAKSAAENRVRFGQRKLERDRDRREQ
                     ERAEKELEDKRLD"
     gene            15076..18192
                     /locus_tag="VGP50_13065"
     CDS             15076..18192
                     /locus_tag="VGP50_13065"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEV8028357.1"
                     /translation="MRKSAIGLAILLFLALAGLILAPALVDLDRLKPRIAAQLAAETG
                     RSVDLLGPLGLSLLPRLTITAHDIRLANLPGAAAKDMVRLRALEVKPAFWSLLKGAFE
                     IRSARLIQPELELERRADGSDNWHFTPAAGAGDVFALALERCAIEDGTVTYRSPGVLE
                     RFEHIDATTALAGPAGPFDASGTLVTHGASLTVALQSGALDAAEVPLRATVSAKPSAQ
                     LELDAVLSGGAGDRRLSGKLKLTAENFQAVATTLASLSVPAVLAQSFALTGELTGSSR
                     GLALDKLALDLGVAHGEGRLDLDFGTPLALALRLSVGRLDLDRWSASHKPALPPAPGV
                     LRGNGAVAPVDPTAPLPLAPQPPPPSVKTSIALGIDSLFWHGGLVRNARLEASLADEA
                     LTLERLTALLPGGAALSLSGSGRLASDGAQAQGLAEVDADDLRSLLGWLGVDASRVPA
                     DRLRKTTLSTRLSLAGDKLELDDIAVSLDTTRLTGAATVVLRERPGIGLRFAADRFNL
                     DAYLPGDAAPDAAVAIDTMSTALRDLDANVDGRVTALTWRGQPLGDVHLAVSLQNQAL
                     SIHDLSIGDLGGAHGAIGGTIDASNGTPKGQLDFELQGPELEHVLRMLSASLATGRLY
                     GEFHASGALRAESDALSLEAALDLLGGHARLAGKLAGGESDLALDLEHPSVAGLLHLL
                     SPDYQPAGDPGELTLSAHLLGTPAHLTIDSLGFAIGPATAEGRAELRTDEARPHVAVN
                     LKLGDWRLDAFMPRRQAAMIDERGRRAGLRPGVILAGVQNPADASRDLGVLALADVDL
                     DVTAQSVVYDKWRLDQAALSGRSADGSLALTRLSGALLGGSIEATGKVEAGAAPALEL
                     RLALKDADLKQALEQIAGVGAIAGRLDAETHLQSAGDNAAELIAHLSGDGVLKGRDGH
                     ITGIDLVAIEDRLSVPERPPDLLALLRSGAGGQTAFSTLGGSFHITDGVMQSDDLRLA
                     AEAGEGRAGGRVDLRQGTIASRVELRFPSITDAPPLAVTIDGSLAEPRVIFETNALQR
                     FLEQRRAH"
     gene            18367..18534
                     /locus_tag="VGP50_13070"
     CDS             18367..18534
                     /locus_tag="VGP50_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028358.1"
                     /translation="MVILLAFYLLIGLLLAGTWTGTAAVRHFRGLAAFLIVTFAWPLI
                     VLGLIVAVRNY"
     gene            complement(18566..>18977)
                     /locus_tag="VGP50_13075"
     CDS             complement(18566..>18977)
                     /locus_tag="VGP50_13075"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HEV8028359.1"
                     /translation="VPSVAAWMRIGRKIGLAEAFALASAIEALGVIDSVLFPTIAGAL
                     VAAALLGVTFIGLTALGLTAVREGAADPRRALGWMTAAFGIGQIIGPAFAGYVSHLTG
                     DFVAASLCAAAALLIAALLAAASRRAPASSAIED"
BASE COUNT         2920 a         6302 c         6746 g         3009 t
ORIGIN      
        1 tcagcgtcgc ctggatattg accgacggga gattggtggc cgcgcgcacg gccaggacga
       61 tgccgcggcg gtcccagcca tgcgcctcca cgtaagtggt gccagcctgc gccgcgctca
      121 gcacgcgcgc cttcatatag ccgccagtgc caagatcgaa catcagcacc agcatggcga
      181 cggcgatcgg catgatcatc gcgaattcga tcgcagcccc gccttcgcgg gcagcgagca
      241 acgacgggtg ccggcgtttg cgcaaggcgg tcattcgacg agctttgcgc cagcggcgga
      301 cgcggcgacg gcacgagccg cgactccggg cgccgacagg aacatcttgg agaaaagcgg
      361 gatctcgggc gcgctgatga cgacctcgat cgccgtcgga tcgctgctgt gcgagcccga
      421 ttgcggcggc tggttgacgg tgacgctcac gccgcctgcg gcatcgatga agccatactg
      481 cgcggcgacc gctctggcag tggcgacgaa atcggcggtg tcgccggcat ggtaggcgat
      541 gagcgcgcta taggcggcgg tgtcggcggc gccctgggcg gcgcgcttgt cgatgtacca
      601 gagcgcgact tcggcgccca agccgacaaa gccgaggagc acggtggcgg acagcgcgaa
      661 catcacggcg acggcgccgc cacggtcgcg gctgagattt cgaatcaatt ttccgagcat
      721 gcgcgcaata tccccgatac gtcgacgaga agcgcgaaga cataccccgg cgagcaagat
      781 agtacgcgat gtcttgcggt cgaattaacc ggagcgcatc gcccggttgc gccgcgaccg
      841 cgggaccacc gccgtttgca gccgcatcgc ccgcggctcg agcggccggc cggtgcgcgc
      901 atcgaccagc accggctcga gcgcggcccc ggtgtccttg tcgacgaccg cgatggtcac
      961 cccgtcgggc ggcgaccagc gctcgcccca ggccatgagc gtgacgagga cgggaaaaag
     1021 gccgcgccct ttttccgtcg gccggtattc gtagcgaggc ggccgggcct cgtaggcgcg
     1081 cttctcgagc agcccctgct cgaccagcca ggccagacgc tgcgtcagga cgttgcgcgc
     1141 gatgccgagc ctttcctgca aggcgtcgaa gcggcgaatg ccgcaggaga tgtcgcggag
     1201 aatgagcagc gtccagcgct cgccgacgat gtcgagcgcc cgggcgatcg gacagggcat
     1261 gtcggagaag cgctttcgct gcatgggacc aatgtaagaa agtgggttgc atcatgcaac
     1321 tcataagact tagctcctcc gcaatgactg agtgccagcg catccttgtc ccgcgatggg
     1381 gcgaaatcct catacgccgg ctgtggttgc cgggggacgg cgccgcctat caccgcttcg
     1441 gcgcggcgct cgcgcccgac gatctgcgcc tgcgcttcgc ggtgccgacg cactggggac
     1501 cggccacggc gcagcgcctg gcgccgctcg acggcacggc ctttgcggct ttcgatcgtg
     1561 gcggcgagat tctcggcatc ggcgacgtcg tggagggcga ggtcgcgctc gctatccgct
     1621 ctgacttaaa gcgccggggt ctcgggcgag cgctgctgga gcgcatccgg gagcacgcgc
     1681 tcgagcgcgg catgaccgag ctggtgggat gcgtgctcgc cgagaaccgt ccgatgctgg
     1741 cgctcgccca gatcgccggc ttccgcgccg cgggcttcga gggcagcatg gtgtcgctgc
     1801 ggctctgcct gccctgacgg cggcgattgg cagcagaagg agtgcgatga tgcggaaagc
     1861 ggtgatcatc ggcgtcgggc ccgaccaagg gctcggcgcc cagctctgca agcgttttgc
     1921 cggcgaaggc ctccacgtct tcgtcgccgg gcgcacgaaa tcggccctcg acgccgtggt
     1981 cgacgatatc gcctcggcgg gcggccgcgc gacgccggtc gtcgccgatg cgaccaagga
     2041 agccgacgtc gtcgcgctct tcaatcaggc cgggggcgac ctcgacctgg cgatctacaa
     2101 cgccggcaac aacacgccgg gacgcatcgt cgagatggag gcggagtatt tcgagcgcag
     2161 ctggcgcgtc gtctgcttcg gcggctttct cttcggccgg gaggcgctgc gccgcatggt
     2221 gccggcaggc ggaagcctgc ttttcaccgg ggccagcgcc tcgctccggg gacgcgcggg
     2281 ctacggcgcc ttcaattcgg cgaaagccgg cttgcgtgca ctggcccagg ccatggccaa
     2341 ggaatacgca aaggacggcg tccatgtcgg ccatgtggtg gtcgacggcg ccatcggcgg
     2401 cgagaagatc cgcaagcgct tcccggagat ggtcaatctc gtcggcgagg acggcctgat
     2461 cggcatcgac ggcatcgtcg atgccttcgt cttcctccac aagcagccgc gcgccgcctg
     2521 gaccttcgaa ctcgacctgc gcacgtcgaa ggagagctgg tgatccggct ggcacgaaag
     2581 aaagataaac gctgtgggcg cggacgttgc gcggaggccg cagaggatat ttgttcctca
     2641 ttgcgggaga gggcgattcg acgcggcttg ttcatacgca aatgtccacg cgacctaatc
     2701 tgcgatccag gcgatgcgca gcgtgttggt ggcgccgggc gtgccgaagg ggacgccggc
     2761 ggtgacgacg atgcgctcgc cggtatcggc gaagccttcc tcgcgggtga tctgcgaggc
     2821 cttcttcacc atgtcgccga aattggtgac gtcgctggtg tgcacgcaat gaaggcccca
     2881 gacgagcgcg agacggcgcg ccgtctgcag attcgaggtc agcgccagga tcggcacgcc
     2941 cggccgctcg cgcgcggtgc ggagcgcggt cgcgcccgag gtcgtgtagg agacgatggc
     3001 agcggcgctc accgtgtacg ccacctggcg agccgcggcg ctgatggcgt cggacgtggt
     3061 gcgctcgggc tcggcttgcg tgaaggcgtg cagattggcg tcgtagagcg ggtcggcttg
     3121 gacgcggcgg atgatgcggt ccatcatggt gaccgcctcg atcggatact cgcccgccgc
     3181 ggtttccgcc gacagcatca ccgcatcggc gccttcatag atcgccgtcg ccacgtccga
     3241 cgcctcggcg cgcgtcggca ccggcgcatg caccatggat tcgagcatct gcgtcgccac
     3301 caccaccggc tttcccgcga cgcggcaggc gtggatgatc tgcttctgca ccggcggcac
     3361 atcctcgggc ggcatctcga cgccgagatc gccgcgcgcc accatgacgg cgtcggtcag
     3421 ctcgacgatc tcggcgagcc gacggacgcc cgcgggcttc tcgagcttca ccatgacgcc
     3481 ggcgcggccg gtgatgagct tcttcgcctc ggccacgtcc tctgggcgct gcacgaagga
     3541 gagcgctatc cagtcgacgc cgagctcgag cgcgtaggcg agatcggtgc ggtccttggg
     3601 cgtcagcgcc gagagcggca ggaccgcgtt cggcacgttg acgcccttgc ggtcggagag
     3661 ctcgccgccg aacaccaccg tcgtctcggc gaaatccgcg ccgcagctct cgacgcgcag
     3721 ccgcacattg ccgtcattga gcagaagctc ggtcccgggc tcgaccgcgg cgaagatctc
     3781 gggatggggc agcggcgcgc gctccagggt gccgggtgtc ggatcgagat cgaggcggaa
     3841 atgaccgccg ccgacgagcc gcaccgggcc ttgcgcgaaa gtgcccaggc ggagcttcgg
     3901 tccctgcagg tccgcgagga tgctgatcgg ccgtcctgtc tccttctcca gcgcgcggat
     3961 tgcgtcgagg cgcgcgcgat gctgctcgtg ggcgccgtgg ctgaagttga gccggaagac
     4021 gtcgacgccg gcgcggaaca aggcttcgat cgttcccggc tccgagcttg ccgggcccag
     4081 cgtggcgacg atcttggcgc tgcgtcgacg gcgcatccct tgtcccctca tcaagccagc
     4141 accaggatgg cgcggaaatc attaacgttg gttcgcgtcg gcccggtgac caccagatcc
     4201 cccagggcgt ggaacgcgcc atagccgtcg ttgtcggcaa gccgcgcgcg caagtcgagc
     4261 ccgagcgtcg ccgcacgcgc gacggtgtcg gggccggcga cggcgcccgc attatcctcc
     4321 gtaccatcga tcccgtcgct gtcgcaagcg atggcatgga ttgcgggatg gccgtcgagc
     4381 gccacggcca gtgccaggag gaattccgca ttcctgccgc cgcgaccccg gccggtgacg
     4441 gtgaccgtgg tctcgccgcc cgagaggagc acggcgggca gcggcagcat acgatcgccg
     4501 acgagaagat gccgcgaagc ggcctggcgg gcgatcgcgg catggacgag cgccacctga
     4561 tgcgcttcgc cctcgatggc gtcgccgagc acgatcggcg tgacgccggc ggcgcgcgaa
     4621 gcggcggcgg cggcggcgag cgcctcctgc ggcgtcgcga tcagcacgaa gcggctgcgc
     4681 gcgaggcgcg ggtcgcccgg cttcggcgtc tcgtcgtgcg cggcggcgag atgcgccgcg
     4741 acggagggcg gcggcgcgat cgcgtatttc gcgaggatcg cgcgggcatc cgcgaacgtc
     4801 gtcggatcgg ccacggtcgg gcccgaggcg atgacggccg gatcgtcgcc cggcacgtcg
     4861 gaaatggcca gggtgacgac cggcgccgga gcggcggccg cggcgaggcg gccgcccttg
     4921 atcgccgaga gatgctttcg cacgcaattg atctcgccga tcgtcgctcc cgagcgcagc
     4981 agcgcgcgcg tgacggcgcg tttgtcctcg agcgtgatgc cggtcgccgg cagcgccatc
     5041 aaggccgagc cgccgccgga cgcgaggaac agcacgaggt cgtcggccgc aagccccgcg
     5101 acgctggcga ggaggcgcgc ggcggcggcg ctccccgcgc tgtccggcac gggatgaccc
     5161 gcttccacca cgtcgatgtc gcggcaggga aggccatggc catagcgcgt gatgacgatg
     5221 cccgagagcg gcccttgcca atgctcctcg acggctttcg ccatggcggc ggcggctttg
     5281 ccggcgccca ggacgaccgt gcgcccgcgc ggcggcggcg ggagatgcgc cggcagcgtc
     5341 cgcgcgggat cggcggcggc caaggccgcg tcgaagagcg cacgcaagaa gcggggggcg
     5401 ttgtcgtggc gcggtgggtc cactgtgttt ccctggcgtc gggccgccgc ccagtctcgc
     5461 gagcgacgcc gggacaagat aggatgggcc gccgccgggc gacaacccgg cgcggcgacg
     5521 ctccggcgcc gccgctcgta tcccgccgcc taccggtcgt gtcgtgcgct cctctcatgc
     5581 tgcttgctgc tgacgatcca ccgccggtcg agtgctgcaa cgaccatgga cgcgcgcccg
     5641 tcctgctcac ttgcgatcac gcgagcgcag cagtgccgcg cgcgctcaac ggccttggcg
     5701 tgccgccggt tgcgatccgg gagcatatcg gctgggacat cggtgccgcc gcggtgacgc
     5761 ggctcctggc gatggcgctc gacgcgccgg cgctgctgtc cggctattcg cgcctcgtcg
     5821 tcgattgcaa ccgggacccc gacgatcgga cgtcgatccc ggccgagagc gacggcgtcg
     5881 ccgtgccggg caaccgcgct ctctcgcccg aggcgcgcat ccgccggctc gaggcgtgct
     5941 ttgctcctta ccacatggcg atcgcggcgc ggctcgacgc cttcgcggcc gccggcgtcg
     6001 ccccggcgtt tctctcggtc cacagcttca cgccggtcat gaaaggcatg gcgcggccgt
     6061 ggcatgtcgg tatcctctgg gacaaggatc cgcgaatccc caagcccctg ctcgcggctc
     6121 tgggcgccga tccgtcgatc accgtcggcg acaacgagcc ctattcggcg cgcgagccgg
     6181 cgggcttcac ggtgcgttat cacgcgatcg cccgcggcct tccgcatgtg gcgctggagc
     6241 tgcggcagga tctcatcgcc gaggccgggg gcgcggcggc ctgggcggcg cgactcgccg
     6301 gcgtgctgcg gccgatcctc gcgcagcgcg cgatctatcg ggtcgagcgc tactgagggc
     6361 ggctatcgcg cgtggcgaga ggagcgccgc accagggcat agccgccgac cgtcagcaac
     6421 agaatggcga gcagcacgcc gctatcgacc acgtagtatt cgggcgaccg atgcttcaac
     6481 aagtcgccgg cgatccaggc gatgccgagg agcggagcgg gcagcagcgc cgcccagacg
     6541 cgtgggaatt gccaaagcgc atgcggagga gtgtttttta tggtcttcat ggcccctagc
     6601 tgaaccgacg cgggtaaaaa aagcgttagc cgggcgcgcc gccgccgagc gccgccggtc
     6661 tggcaaacgc cgcgccggac cggcaagatg caggggccgc gattcaagga gatagcgatg
     6721 gacgacaaga cgcgaatcga gctcgaggcg ggcgccttcc gccggctcgt gcagcatctg
     6781 cgcgagcgca ccgacgtgca gaacctcgcg ctgatgaacc tcgccggatt ctgccgcaat
     6841 tgcctcgcgc gctggtaccg cgaggaggcc gagtcgcggg gaatcgcggt cgccgacccc
     6901 gaggcgcgca agatcgtcta cggcatgccc tatgacgaat ggaaggcgaa gtaccagaag
     6961 gacgagaaaa agccggcctc cagcgggggc caccagcact gacggtcgca ggcgcggcgc
     7021 tttgccttgc gcctctcgtg gccctccgat acggtcgatg caacatccaa ccacggggga
     7081 gtccacatgc cggatgtcgg cggcattgcc ggtgagcgtt tgcgatcctt catcgagcgc
     7141 atcgaacgac tcgaggaaga gaaacgggcg ctcgcccagg acatcaagga agtctacgcc
     7201 gaggcgaagg gcaccggctt cgacgtcaag atcatgcggc agatcgtcaa gatccggaag
     7261 atggatcaga acgagctcga cgagcaggag actctcctcg acgtctacaa gcgcgcgctc
     7321 ggcatgaccg tcgcattcgc cgaagaggcg gcggagtagc tcacgtcatc ctaatgccat
     7381 cgtgacgagc tcggtgtcgg cgtagtccgt catctccgtc accttgccgc cggcgatgcg
     7441 cgcgacgatg caatacgtat tgttgtaggg cttgccgctc ctcgatgtgg cgcggccgcg
     7501 ggcctgcatg acgacggcgt cgccctcggc gatgagacgg tcctgggtaa agacgatcgg
     7561 gccggccaga cgcacgcgca gcggcttcaa cagtttgtcg atgatctcct gcttgccgca
     7621 gtatgttccg gaaagcgccg tcgtgccgat gatggtccat cgcacatcct cggcgaggct
     7681 gtcgagcagc gtttggacat tgcctttcga gagctcttca tagatgtgcc gcagaagttg
     7741 cttattcgtt tcggtgctca tgcgcttgct ccggtagcgc ggaataggtg ctgggtacgg
     7801 acttagtatg caggcgctgg gtaattttgt caaactcagg taatctggcg cccatgcagc
     7861 cgagccgatt tctttacgat ctggagaact gctccattcg ccgcgtcctc gatctcctcg
     7921 gcgagaagtg gacgttgctg gtgctgcgcg aagctgttta tggcgtgcga aggttcgacg
     7981 atttcgcgcg cgcgctcggc tgcggccggg gcgtgctgag cgaccggctg aggaccctga
     8041 cggatgcgga tatcctggcg cgagtcgaat accaggagcc caatcagcga gcgcgcgccg
     8101 agtatcatct gacggacaaa ggacgcgatc tgtttcccgc ccttctcgcg ctctcccaat
     8161 ggggcgatcg ttggatgccg ccggcgggcg gcccggtcgc gcgcgtgacc catcggaagt
     8221 ccggtcgtcc ggtcgcggtg gtgatgacgg gcgatccgaa agtggaagcg ctgacgatgc
     8281 gcgacgtcga gatcgcgccc ggtcccggcg cgaagcgcat cggtcgagcg cgttgacgcc
     8341 gggcgacgaa cgttacgagg cgaacctcgg gccgcgaatg tcagagtccg acctcaacat
     8401 ccgtcatccc cgctttcgcg gggatgacga tctttaggat gatgtagcgt tactccgccg
     8461 cgatggcgtg gggggcgggg ggtgagcgga aggcggcgag cagcttctcc tggcgcgcgc
     8521 gggcgtcggc gagattggcg gccttcacat ggccgaagcc gcggatctgc tgcgggatgc
     8581 gcgcgagctc gacggcgatg ccgtggttct cgggggtgag gccggggatg agctcgtcca
     8641 ccagcgcttc gtactccgcg acgagacggc gctcgacgcg gcgctcctcg ctccggccga
     8701 agggatcgag cggtgtgaag cgcagaaact tgagcttcgc gagcaggcgg aaggccgtca
     8761 tcatccaggc accgtagcgg cgcttcttct ggtgaccggt caggggatcg cgctctgcca
     8821 gcagcggcgg cgccagatgg aactcgaggc tgaaatcgcc gtcgaattgc tgcgcgagtt
     8881 tcttctcgaa cgagccgtcg gtgtagagcc gcgccacctc gtactcgtcc ttgtaggcca
     8941 tcagcttgaa gaaattgcgg gcgaccgctt cggcgagacc ctggtggccg ggcgcgcgca
     9001 actcctcggc ggcctcggcg cggcgcacga gacggcgata gcgctcggcg taagccgcgt
     9061 cctgatagtc ggtcaggaag gccacgcgcc gcgcgaccat ctccgcgacg ctctccgaca
     9121 ggcgctcgtc ggccgccgct tccggcgtgg cgatccgctc gaccgcttcc cggtcgagcg
     9181 cggcccagcg gccgtagcgg aaagcgcgat gattgaaatc gacggcgacg gcgttgagcg
     9241 cgatggcgcg ctcgatggcg gtggcggaga cggggacgaa gcccttctga taggcgaagc
     9301 ccagaaggaa gagattggtg gcgatggcgt cgccgagaag cgcggtcgcg agccgcgtgg
     9361 catcgaggaa atcagcattc tcgcggccga ccgcgccggc gatcttttgc gcgaggtcgc
     9421 ggccgggaat ggcgaaatcg gggttgcggg taaagtcgcc ggtgatgatc tcgtgctcgt
     9481 tcacgacggc gcgggcatcg cgtccgccga ggcgcatcag actgtcgggc gtcgccgcga
     9541 cgacgatgtc gcagccgagc agcagccgcg cgcggcccag ggcgatgcgc gcggcatgga
     9601 ggtcgtcggg cgcgccggca aggcggatat ggctcatcac ggcgccgccc ttctgcgaca
     9661 gcccggtctg atcgaggatg gagcagcctt tgccttcgag atgcgcggcc atgccgagaa
     9721 gcgcgccgat ggtgacgacg ccggtgccgc cgaccccggt cacgaggatg tcgtaaggct
     9781 ccgccagcgc cggcaaggac ggctcgggca gcgccgggat ctcgtcctgt ccttgaggcg
     9841 catggtggcg gcggagggtt ccgccatgca cggtgacgaa gctcgggcag aagccctcga
     9901 cgcaggagaa gtccttgttg cagctcgact ggtcgatggc gcgcttccgg ccgaactcgg
     9961 tctcgagcgg cacgacggag aggcagttcg aggtgcgcga gcaatcgccg cagccttcgc
    10021 agacgaggtc gttgatgaag acgcgcttct gcgggtcggg gaaggtgccg cgcttgcgcc
    10081 ggcgccgctt ctcggcggcg caggtctggt catagacgag gacgctgacg cctgtggtct
    10141 cgcgcaggtc gcgctgcacc gcgtcgaggc gctcgcgatg gaagacggcg acgtctggag
    10201 cgaatccggc attgaccgga tatttgtcag gctcgtcggt gacgacgacg atgcgcgtca
    10261 cgccctcggc cgcgagctgc cgcgtgatct gcggcacggt gagctgcccc tcgatgggct
    10321 ggccgccggt catggcgacg gcgtcgttgt agagcagctt gtaggtgatg ttgacgcccg
    10381 cggcgagcgc agcgcggatc gcgagaatgc cggaatggaa gtacgtgccg tcgccgagat
    10441 tggcgaagac atgcggcgtc tcggtgaagg gcgcctggcc gatccaggtc gcgccttcgc
    10501 cgcccatctg cgtgacggtg gcggtgtcgc ggtccatcca gatcgccatg tagtggcagc
    10561 cgacgccgcc ggtggcgcgg ctcccttcgg ggacgcgcgt cgaggtgttg tgcggacagc
    10621 ccgagcagaa atagggcacg cgttcgatcg cgggtttgcc ttgctgctgg cgctggagcg
    10681 cggtgaggaa gttcaggcgc tcggtgaggc gcggcgtcgg atggaagcgc tggatgcgct
    10741 cggcgagaac gcgcgccact tgcaccgggt cgagctcgcc ggtcgagcgc aggagcggcg
    10801 cgccgtgctc atcctccttg ccgaggatgc gcgggcgctc gctcctgttg tagaggatgt
    10861 ccttgacctg ctgctcgacg acgggacgct tctcctcgac gaccaggatc tcttcgaggc
    10921 cttgggcgaa gcggcgcacg ccctcgggct cgagcggcca ggtgagcgcg accttgtaaa
    10981 ggcgcaagcc catggccgcg gcttgcgcgt cgtcgatgcc gaggtcggaa agcgcctggc
    11041 gcagatcgag ataggacttg ccggtggtga tgatgccgag acgcgggcgg ggcccatcga
    11101 tgaggacgcg gtcgaggcgg ttggtgcgcg cgaaggcgag cacggccggc agcttcccgt
    11161 cgcgcaggcg gcgctccatc tcaagcgcgt tgtggtcggg ccagcggatg ttgaggccgc
    11221 cgggcggcag cgcgaaatct tccggcagcg cgatctggac gcgggcgggg tcgacgaaga
    11281 cggaagccga gctttcgacc gtctcggtga tggtcttcag gccgacccac aagccgctga
    11341 agcgcgagag cgcccagccg aaaaggccga aatcgagaat gtcctggatg ccggcgggat
    11401 tgagcaccgg gatcgacgcg tcgatgaagg cgtattccga ttggtgcgcc agcgtcgagg
    11461 atttggcggc gtggtcgtcg ccggcgagca cgaggacgcc gccatggcgc gacgtgccgg
    11521 cggcattggc atgcttgaag acgtcgccac tgcggtcgac gcccggacct ttgccgtacc
    11581 acatggcgaa gacgccgtcg tatttggcgc cggggaacat gctggtctgc tggctacccc
    11641 agacggcggt cgccgcgaga tcctcgttga tgccgggctg gaaacggatt tggtgctgct
    11701 cgaggaagcg ctgggcctgc cagagattct ggtcgaggcc gcccaagggc gagccgcgat
    11761 agcccgagat gaagcccgcg gtgtcgagcc cggccgcgcg gtcaagccgc cgctgcatca
    11821 tcggcaggcg caccagcgcc tggattccgg agagatagat ccgcccttgc tcgagggtat
    11881 atttgtcgtc gagactgact gccgccagcg ccatcggccg ctccaggcgt cgttctaagt
    11941 cttcctccga taacaaacac ttaatgcggt cggttcgcgc gctcaattgc gcgcgcggcg
    12001 taagatcgat ttcgcaacga aagtgccgcc gctgcgcgtc cggcacggca aatctgtcgt
    12061 atagcaaccg ttgcgcgcat gattctctct ttgtcgatgg cgcgtcagcg aggcaatcgg
    12121 cgaaaccgcg gttgaatcgc cgcaaagttt ggcgttaggc tcctgcgcca aggggatttt
    12181 gcacggccga aagaatcgtc gatgaccgcg cctttgttgc gccgcaccga aatgagcgac
    12241 gccgtgatcc ttggcctgaa ggagcgcttc ggcgagcgcg tctcgacagc ggccgcggtg
    12301 cgcgagcagc acggcaagga cgagagctat cacgcgccgc ggcagcccga cgcggtggta
    12361 ttcgcccgct cgaccgacga cgtcgtcgac atcgtcaagc tctgtgcggc gcaccagacg
    12421 ccggtcattc ccttcggcac gggcacgtcg ctcgaaggcc atgtggcggc gctcgaaggc
    12481 ggcgtgtgcg tcgacctttc ggcgatgaac cgcgtgctgc gcgtctcggc ggccgatctc
    12541 gactgcacgg tcgaggccgg ggtcacgcgc aagcagctca acacgcatct gcgcgacacc
    12601 gggctcttct ttccgatcga tcccggcgcc gacgcgtcct tgggcggcat ggcggcgacg
    12661 cgcgcctccg gcaccaacgc cgtccgctac ggcaccatgc gcgagaatgt gctggcgctc
    12721 accgtggtgc tggccgatgg gcgcgtgctg cgcacggcgc ggcgcgcccg gaagtcggcg
    12781 gcgggctacg acctgacgcg gcttttcatc ggctcggaag ggacgctcgg cgtcatcacc
    12841 gaagtgacgt tgcgcctcta cggcatcccc gaggcgatca tggcggcggt ctgccccttc
    12901 ccgtcggtcg aagcggcggt cgatacggtg gtcgcgacga tccaggccgg cgtgcccatc
    12961 gcccgcatcg agctcctcga cgcgaagcag atggaggccg tcaacaaata cgcgaagctc
    13021 gaccacgccg tggcgccgac cttgttcttc gagttccacg gcacgacgcg cggcgtcgag
    13081 gagcaggtgg cgcaggtgaa ggccttcgcc gcggagcagg gcggcaacga cttccactgg
    13141 gcggcgaccg ccgaggagcg ctcgaagctc tggcaggcgc ggcacgatgc ctattacgcg
    13201 gccctggcgc tgcgtccggg cgccaagggc tgggcgaccg atgtctgcgt gccgatctcg
    13261 cggctcgccg aatgcatcgc cgagacgcgg cgcgatgtcg atcagtcctt cgtcacggcg
    13321 gtgctggtcg gccatgtcgg cgacggcaat tttcatctca tcttcgtgct cgatccgacg
    13381 aagcccgagg agttcgccga ggcgagccgg atcaacgacc gcatggtggc ccgcgccatc
    13441 gccatggacg gcacctgcac cggcgagcac ggcatcggct acggcaaaat ggatttcctc
    13501 atcgccgagc atggcgaggg cgtcgcggtg atgcgcagcc tcaaacacgc gctcgacccc
    13561 gacaacatca tgaatccggg gaagatcgta cggatgtgat cggtcttgcg agatgcgcgc
    13621 acgcacccct caccctcccg ccgcttcgcg gcgggcccct ccctctccca cgaggggaga
    13681 gggatttttt taacgcgctt gcttaaatcc cctctctcag ctcgcgcgtt cggggtagcg
    13741 gatggagagg atctcgagct cgacttcgcc ggcggggcgg cgccacagca cgacgtcgcc
    13801 gacgccggag ccgatgagcg cgcgggcgag cggcgagacc cagctcacct tgccatgctc
    13861 ggcatccgcc tcgtcctcgc cgacgatggc aaattcctgg cgctcgcccg cggcatcgac
    13921 cacgcgcacg acggcgccga aggcgacctc gtcggccggc gcctgggcag gatcgaccgg
    13981 gatcgcccgc tcgatccgcg cctcgaggta gcggatctcg cgcgccgtgc gcgcggcttc
    14041 gaggtccttt gccgggtcgt cgggtcgcgc ggagaggcgg cgctgctgct gctgcgcgcc
    14101 ggcgaggcgc tcttcgagga ggcgcagacc gcgccccgtg acataattgg ggtgggtgct
    14161 ctgcggcaat tccgggagca tatcggccgc cgcatccgtg tcgctctcac tgacgaaggc
    14221 tcgactcatg acggttctcc tcgaaaacgc tccaagggca acggtgcgac gcctctcagg
    14281 ttccgcggcg gtggcggggg aggggtgaca tggcgcccga cccgcggatc gctctggcga
    14341 ccggcgacat caccaaattg gcggtcgacg ccatcgtcaa tgcggccaac agctcgctcc
    14401 tgggcggcgg cggggtcgac ggcgcgatcc atgacgcggc cggtccggga ctgcaggccg
    14461 aatgcctcac gctgggcggc tgcccgaccg gcgaggcgcg gatcaccagc ggtcatcgcc
    14521 tgcgggcgcg cttcgtgatc catacggttg ggccggtctg gcgtggcggg cgcgcagatg
    14581 agtgggaact cctcgctcgt tgctatcgct cgagcttcgc gctggcgagc tcgcatgcca
    14641 ttcgctccat cgcctttcca gccatttcca cgggagctta tcgttttccc gctctccagg
    14701 cctgcgctat tgccgttcat gagacccacc acttcttggc gaggaacgac acactcgaaa
    14761 aggtgatctt cgtcgccttc gatgccgaga tcgaagaact ctatcggcgc gcgctcggcc
    14821 tcctctaatc gagccgcttg tcctcgagct ccttctcggc gcgctcctgc tcgcgacggt
    14881 cgcggtcgcg ctcgagcttg cgctggccga aacgcacgcg attctcggcg gcgctcttgg
    14941 ccgcctcctt gcgcaggcgc tgcttgcggt agcggttgag attgacgatc tcagccatgg
    15001 cgacctcccc gccggctcgg ctggcggccg aggcgcggca tgctatggtg gaacgacccg
    15061 gtgcggaggc gttccttgcg caaatcggcc atcggcttgg ccatcctttt gttcctcgcc
    15121 ctggccggtc tcatcctcgc ccccgcgctc gtcgatctcg accgcttgaa gccgcggatc
    15181 gcggcacaat tggcggccga gacaggacgc agcgtcgacc ttctggggcc gctcgggctg
    15241 tcgctgctgc cgcgattgac gatcaccgcc cacgacatcc gcctcgccaa tctccccggc
    15301 gcggcggcca aggacatggt acgactgcgc gccctcgaag tgaagccggc cttttggtcg
    15361 ctccttaagg gtgcgttcga gatcaggagc gcccgcctta tccagccgga gctcgagctc
    15421 gagcggcggg cggacggcag cgacaactgg cacttcaccc cggcggcggg cgcgggcgat
    15481 gtcttcgccc tcgcgctcga gcgctgcgcc atcgaggacg gcaccgtcac ctatcgctcg
    15541 cccggcgtgc tcgagcgctt cgagcatatc gatgcgacga ccgcgctcgc cggtcccgcc
    15601 ggtcctttcg atgcaagcgg cacgctcgtc acgcatggcg catcgctgac cgtcgcgctg
    15661 cagagcggcg cgctcgatgc ggccgaggtg ccgctgcgcg cgacggtgag cgccaagccg
    15721 tcggcgcaac tcgagctcga cgcggtgctg agcggcggcg cgggcgatcg tcgcttgagc
    15781 gggaagctca agctcacggc ggagaatttc caggcggtgg cgacgacgct ggcaagcctg
    15841 tcggtgccgg cggtgctggc gcagtccttc gcgctgacgg gcgagctcac gggctcgtcg
    15901 cgcgggcttg cgctcgacaa gctcgcactc gatctcggcg tggcgcatgg cgaaggccgg
    15961 ctcgacctcg atttcgggac gccgctcgcg ctcgcgctca ggctctcggt cggccgactc
    16021 gatctcgatc ggtggagcgc gtcgcacaag ccggcgttgc cgccggcgcc gggggtgctg
    16081 cgcggcaacg gcgccgtcgc accggtcgat cccaccgcgc cgctgccgct cgcgccgcaa
    16141 ccgccgccgc caagcgtcaa gacgagcatc gcgctcggca tcgacagcct gttctggcac
    16201 ggcggcctcg tccgcaatgc gcggctcgag gcaagcctcg ccgatgaggc gctgacgctc
    16261 gagcggttga cggcactcct gcccggcggc gcggctctgt cgctgagcgg cagcggcagg
    16321 ctcgcgtccg acggcgcgca ggcgcaaggc ctggccgagg tcgatgccga cgacttgcgc
    16381 agcctgctgg gttggctcgg cgtcgatgcg tcgcgcgtcc cggccgatcg cttgcgcaag
    16441 acgacgctgt cgacgcgcct ctcgctggcg ggcgacaagc tcgagctcga cgacatcgcg
    16501 gtgagcctcg acacgacacg gctcaccggc gccgccacgg tcgtgctgcg cgagcggccg
    16561 ggcatcgggc tgcggttcgc cgccgaccgc ttcaatctcg atgcctatct gccgggcgac
    16621 gcggcgccgg atgccgccgt cgccatcgac acgatgagca ccgcgttgcg cgacctcgac
    16681 gccaatgtcg atggacgagt cacggcgctg acctggcgcg gtcagccgct gggcgacgtg
    16741 catctcgccg tctcgctcca gaaccaggct ctgagcatcc acgatctcag catcggcgat
    16801 ctcggcgggg cgcatggcgc catcggcggc accatcgatg catcgaacgg aacgcccaag
    16861 ggccagctcg atttcgagct ccaaggaccc gagctcgagc atgtgctgcg catgctgtcg
    16921 gcgagcctcg ccaccggtcg cttgtatggc gaattccacg cgagcggggc cttgcgcgcg
    16981 gagagcgatg cgctctcgct cgaagcggcg ctcgacctgc tgggcggcca tgcgcggctg
    17041 gcgggcaagc tcgccggcgg cgagagcgac ctggcgctcg atctcgagca tccgagcgtt
    17101 gccgggctgc tgcatttgct gagcccggac taccaacccg ccggcgatcc cggcgagctc
    17161 acgctctcgg cgcatctcct cggcacgccc gcccatctca ccatcgactc actcggcttt
    17221 gccatcgggc cggcgacggc ggaggggcgt gccgagctgc gcacggacga ggcgcggccg
    17281 catgtcgcgg tcaatctgaa actaggcgac tggcgcctcg acgcgttcat gccgcggcgg
    17341 caggccgcga tgatcgacga gcgcgggcgg cgtgccgggc tcaggcccgg cgtcatcctc
    17401 gccggggtgc agaaccccgc cgacgcgtca cgcgatctcg gtgtactggc gctcgccgat
    17461 gtcgatctcg atgtgacggc gcaaagcgtc gtctatgata agtggcgcct cgaccaggcc
    17521 gcattgagcg gccggagcgc ggacggctca ctcgcgctca cgcggctgag cggcgccctt
    17581 ctcggcggga gcatcgaggc gaccggcaaa gtcgaggcgg gcgcagcgcc ggcgctcgag
    17641 ttgcgccttg cgctcaagga cgccgatctc aagcaggcgc tcgagcagat cgcgggcgtc
    17701 ggcgcgatcg cgggccggct cgacgccgag acgcatctgc aaagcgcggg cgacaatgcg
    17761 gcggagctga tcgcgcatct ctccggcgac ggcgtgctca agggccgcga cgggcacatc
    17821 accggcatcg atctcgtcgc catcgaggat cggttgagcg tgccggagcg cccgccggac
    17881 ctgctggcgt tgctgcgcag cggcgccggc gggcaaaccg cgttctcgac gctgggcggc
    17941 agcttccaca tcaccgacgg cgtgatgcaa agcgacgatc tgcgcctcgc ggccgaagcc
    18001 ggcgaggggc gggcgggcgg ccgcgtcgat ctgcgccagg ggacgatcgc gagtcgcgtc
    18061 gagctgcgct tccccagcat caccgatgcg ccgccgctgg ccgtcaccat cgacggttcg
    18121 ctcgccgagc cgcgtgtcat tttcgagacg aacgcgctgc agcgcttcct cgagcagcgg
    18181 cgcgcgcatt aacggccgcg cttcggcaat gtcgtttcgg cgctgcgcaa accctttaac
    18241 catcttgcgt cccgatccgg tcagactcac atcggagtga gcaggcggtg ccacaaaacg
    18301 actggaacat tttgcggttt ggtgccgtta taaaatgcaa ccggtggtcg acaggatttg
    18361 cgctccatgg tcatcctttt ggcgttttac cttctcattg gcctgctgct tgccgggacg
    18421 tggacgggaa cggcggcggt ccggcatttc cgcgggctcg ccgccttcct catcgtcact
    18481 tttgcctggc cgctgatcgt gctgggcttg atcgtcgccg tccgcaatta ctgagccgcc
    18541 ggcgggcagc cgcgcccgcc ttggttcaat cctcgatcgc gctcgaggcc ggcgcccgcc
    18601 ggctcgcagc cgccagcagg gcggcgatca gcagcgccgc cgccgcgcag agcgaggccg
    18661 cgacgaaatc gccggtgaga tgcgagacat agccggcaaa ggccgggccg atgatctggc
    18721 cgatgccgaa agcggcagtc atccagccga gcgcgcggcg cggatcggcc gcgccttcac
    18781 gcaccgcggt gaggccgagc gcggtcaggc cgatgaaggt caccccgagc aaggccgcgg
    18841 caacgagcgc tcccgcgatc gtgggaaaga ggacgctgtc gatcacgccg agcgcttcga
    18901 tcgccgaggc gagcgcgaag gcctccgcca ggccgatctt gcggccgatg cgcatccagg
    18961 cggcgaccga tggcacg
//
LOCUS       DASYNS010000420        10171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_731449, whole genome shotgun sequence.
ACCESSION   DASYNS010000420 DASYNS010000000
VERSION     DASYNS010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731449"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VGP50_13080"
     CDS             complement(<1..1124)
                     /locus_tag="VGP50_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8028360.1"
                     /translation="MALALDSDATLLRDTALEFFRERAPVAALRKLRDDNDPTGFSRP
                     LWREMAALGWAGFLVDEEHGGSAFGCLGLGLVMEAAGRTLAATPLLSTALIGASALSL
                     AGSAAQKSAHLPGLVAGERLTALALEEGAHHAPFRIAATAKRSGKGFRLDGEKRFVLD
                     GHVADLLIVVARTAGSGEERQGLTLFLVPADAAGLERRRTDMVDSRNAATVRLDGVTL
                     GADAVLGAVDGGAEILEQILDRARAALAAEMLGGAGEAFERTVQYLKDRQQFGVRIGT
                     FQALKHRAAQMFCEIELARSAVVAALGAIDAGAGNARELASIAKAKAGDAFFLVSNEG
                     IQMHGGIGMTDEHEIGFFLKRSRVAQASFGDSTFHRDRYAA"
     gene            complement(1135..2319)
                     /locus_tag="VGP50_13085"
     CDS             complement(1135..2319)
                     /locus_tag="VGP50_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8028361.1"
                     /translation="MQDLDAFRAEAHAWLEANCPRSMRAPMPEDEVVWGGRRASFKNP
                     DSRIWLERMAEKGWTVPGWPKAYGGGGLSSAETRILEDEMARLGCRAPLLSFGIWMLG
                     PVLLEFGNEAQKQEHLPKIARGEIRWCQGYSEPEAGSDLAGLKTRAVPQGEHFLVNGH
                     KVWTSYANYADWIFCLVRTDALAKKHDGISFILIDMADPGVRTRPIRLISGSSPFCET
                     FIENVRVPMTNLVGGLNQGWTIAKRLLEHERKMIASIGERQRGAERSLGEIGKSYVGA
                     REGKLADATLRDRIAQQEMDAQAFQLTRRRAAEEAKTGTPGPASAMFKYYGTELNKRK
                     FELLLQCVGTQALGWSGDGFNPDELRITREWLRSKGNSIEGGTSEVQLNVIAKRVLGL
                     PD"
     gene            complement(2454..2702)
                     /locus_tag="VGP50_13090"
     CDS             complement(2454..2702)
                     /locus_tag="VGP50_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028362.1"
                     /translation="MGLTDKLRKYARRLSRPMVKQWLSDMLVDSSVHDVQGAKSSLAL
                     RGFGGNAANGDRYVKEASQAIEIRVTFSGGLQQTVTRL"
     gene            2701..3240
                     /locus_tag="VGP50_13095"
     CDS             2701..3240
                     /locus_tag="VGP50_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028363.1"
                     /translation="MSSPSDADDAAPLAPIARLVATIKAERAACAAPAIGGEERDRLG
                     AELRQLIAAREEEQRAWRDAMDVLKTDLDATRAAARAAETRHEQAAQEHRRVMADLEL
                     KHEHQRSIWQLDRRRLEITIAGQETAQRARSSWLAVAVGLLVVGVFGAALVADHRVGP
                     PAEDAAAAQTVFPASGSSR"
     gene            3318..3596
                     /locus_tag="VGP50_13100"
     CDS             3318..3596
                     /locus_tag="VGP50_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028364.1"
                     /translation="MRGRLLLLVVAACLPMTAARADMMGDEFKRNSSVWRNNDLCARQ
                     AFRQFPDYTPEGNAKRDHAMQQCLAATHAPPRDSTNPKPAAAPDQQPQ"
     gene            3602..4597
                     /locus_tag="VGP50_13105"
     CDS             3602..4597
                     /locus_tag="VGP50_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEV8028365.1"
                     /translation="MADGAPLLFPLAGRRVFVAGHRGMVGSALVRRLARAGCTVLTAE
                     RSIDLRRQDATEAWLGAERPEAVFVAAATVGGILANDTRPADFLYDNLMIAGNVIEAA
                     RRSGVRKLLLLGSSCIYPRLAPQPMTEDALLSGPLEPTNQWYAVAKIAGLKLCAAYRR
                     QYGLDFISAQPTNLYGPGDTYDLRASHVVPALIAKTHRAKVTGAGDVEIWGTGAPRRE
                     FLHVDDLADALVFLMERYSDELHLNIGWGEDVSIRELAEVIAEVVGFTGRFRYDRTKP
                     DGAPRKLLDVTRLSALGWRPRIALREGLTDAYRWFLESLSPLAGEGGARREASGG"
     gene            4684..5577
                     /locus_tag="VGP50_13110"
     CDS             4684..5577
                     /locus_tag="VGP50_13110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV8028366.1"
                     /translation="MSASPPRVSVVIPLYNRAHTIARALASVERQRFGDFEIVVVDDA
                     SSDGGAGTIGGRDPRLRILRHEANRGAAAARNTGVDAARGEYIAFLDSDDEWLPEKLA
                     TQIAVLDRRGRGCDAVVSAFILVDEPSGRRHAVRAPFRRDPRRSLLLGCAVSPGSTLV
                     CRRDYLVGGHLFDERLRRLEDWDWLLTYVRERDLALIDEPLAIIHGNAGAHGPFVLAA
                     LETIEDRHGGAIAALGPSARRMFRSTLLLERAAAAYRSRDWRRSASMLWRSLLLYPFR
                     WDFFARMLQRLLPRRHQGPLP"
     gene            5636..6586
                     /locus_tag="VGP50_13115"
     CDS             5636..6586
                     /locus_tag="VGP50_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV8028367.1"
                     /translation="MTVESEDIRRLYRAMKRIRHVEEEIARLYPSDKIKSPVHLSIGQ
                     ESVAVGICDPLRADDVVSGTYRGHAAYLAKGGSLPALMAELYGKVDGCAAGKGGSMHI
                     IDMEHFILGTSAVVGSTIPVAVGYALAMKREGRGRVVACFFGDGATEEGVFAESLNFA
                     ALKHLPVLFVCENNQYAIHTHTSRRWATDQLCARVATYGIPVEQVADGDVLAIRARAG
                     KAYAAMRDGGGPAFLECRTYRWREHVGPGEDYDAGYRRRAELEEWQRRDQIARLAAML
                     APAERGAIDAAIEAEVAAAVDFAEKSPFPPVEQLKTDVFA"
     gene            6573..7637
                     /locus_tag="VGP50_13120"
     CDS             6573..7637
                     /locus_tag="VGP50_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEV8028368.1"
                     /translation="MSSPDGTERVLSYVDALREAVAQEMERDPRVFVIGLDVDDHKAI
                     QGSTRGLLERFGPERVMGTPLSEEAMTGVAIGAAMAGLRPIHVHIRMDFLLLCMNQLI
                     NIAAKARYMYGGQVAVPLVVRSMIGKSWGQGAQHSQGLHSLFMHVPGLKVVAPADAYM
                     AKGCMIAAIRDDNPVIFVEHRLLYFTDAVVPVEPYAVEFGRARLCTEGSDITIVGVSN
                     MLIECLRANELLKSAGISAEVIDPVSLVPLDLATISASVARTGRLLVVDNAWTTCGAS
                     AEIIAGVVEHAAGERIVARRMGYARTSCPTTPSLEQAFYPNPATIAETALHMVRPHAT
                     WRPDPEEARLAYQARFRGPF"
     gene            7792..8961
                     /locus_tag="VGP50_13125"
     CDS             7792..8961
                     /locus_tag="VGP50_13125"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEV8028369.1"
                     /translation="MFYELAASTWGQEEHDAIARVVKSGRFTMGPEVAAFEDAFARYF
                     GHKYAVMVNSGSSANLIAVASLFYKKERPLERGDEVIVPAISWSTTYHPLQQYGLKLR
                     FVDVDLATLNIDASQLERALTRKTRMIVAVSILGNPAPLDVMRRFADKHGLYFIEDNC
                     ESMDAELAGRKTGTFGDVATYSSFFSHHISTMEGGMLATDDPELWELARSLRAHGWTR
                     DLPEGSKLYEKGDDDFFEAYRFILPGYNVRPLELSGAIGIEQLKKLTGMTAARRKNWA
                     TFTSLFAGDERFVIQRENGKSSAFCFTIVLDPKRRPERRRVLAALKDADIGYRIITGG
                     CFTRHEVIRHYDYTTVDDLPNANLAHDLGFFVGNHPFDLTPQIERLRGVLDRVCN"
     gene            8971..9918
                     /locus_tag="VGP50_13130"
     CDS             8971..9918
                     /locus_tag="VGP50_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8028370.1"
                     /translation="MTGERYSILVTGGAGFIGSILVPALLEAGHKVTVLDNFMYRQDS
                     LNHVCADPNFAVARGDARDEAVLRPLVAKADAVIPLAALVGAPLCDRDKIGATSTNRD
                     GVATLVRLLSKEQRVLYPNTNSGYGVGDKGQYCTEESPLRPVSLYGRDKVAAEEIVLG
                     HGNAVVFRLATVFGMAPRMRIDLLVNDFVYRAVTDRFIVLFEGHFKRNYIHIRDVARA
                     FLHGIDHLAEMRGRTYNVGLSDANLSKIELCALIKRFLPQLVYLEAPVGEDPDKRDYI
                     VSNARIEATGFKPKHSLDLGIAELIKGYAMIRNSIYGNM"
     gene            complement(9947..>10171)
                     /locus_tag="VGP50_13135"
     CDS             complement(9947..>10171)
                     /locus_tag="VGP50_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028371.1"
                     /translation="VLVAAPAQARYLARWSRWLCRAPCETRVIADLAQLERSLTEAAP
                     PRRVLIAGELYARFHVRLAADVGMGRLAVA"
BASE COUNT         1595 a         3436 c         3467 g         1673 t
ORIGIN      
        1 gcggcgtagc ggtcgcggtg gaaggtggag tcgccgaagc tcgcctgcgc gacccgcgag
       61 cgcttcagga agaagccgat ctcgtgctcg tcggtcatgc cgatgccgcc atgcatctgg
      121 atgccttcgt tgctgacgag aaaaaacgcg tcgccggcct tggccttggc gatgctcgcg
      181 agttcgcggg cattgccggc cccggcgtcg atcgcgccca gcgccgcgac cacggccgag
      241 cgcgcgagct cgatctcgca gaacatctgc gcggcgcgat gcttcaacgc ctggaaggtg
      301 ccgatgcgca cgccgaattg ctggcggtct ttgagatatt gcacggtgcg ctcgaaggcc
      361 tcgccggcgc cccccagcat ctccgcggca agggctgccc gcgcccggtc gagaatctgc
      421 tccaagatct cggcgccgcc atcgaccgcg ccgagcacgg cgtccgcgcc gagggtgacg
      481 ccgtcgagac ggaccgtggc ggcgttccgg ctgtcgacca tgtcggtgcg gcggcgctcg
      541 aggcctgcgg catcggccgg gacgagaaag agggtgaggc cctgccgctc ttcgccgctt
      601 cccgccgtgc gcgcgacgac gatcagcaga tcggcgacat ggccgtcgag aacgaaccgc
      661 ttctcgccgt cgaggcggaa gcccttgccg ctgcgtttcg ctgtcgcggc gatacgaaag
      721 ggcgcgtgat gcgcgccttc ctcgagggcc agtgccgtga gacgctcgcc cgcgacgagg
      781 ccggggagat gcgccgattt ctgcgcggcg ctgccggcga gcgagagcgc gctcgcgccg
      841 atgagcgcgg tcgacagcaa gggggtcgcg gcgagcgtgc gcccggccgc ctccatgacg
      901 aggccgaggc cgagacagcc gaaggccgag ccgccatgtt cttcgtcgac gaggaacccc
      961 gcccagccca acgcggccat ctcgcgccag agggggcgcg agaatccggt cggatcattg
     1021 tcgtcgcgca gcttgcgcaa cgccgcgacc ggcgcgcgtt cccggaagaa ttcgagcgcg
     1081 gtgtcgcgca gcagcgtcgc gtcgctgtcg agagcaagag ccatcgtcgc gcgctcagtc
     1141 gggcaggcca agcacgcgct tggcgatgac gttgagctgg acctccgagg tgccgccctc
     1201 aatggaattg cctttcgagc gcagccattc gcgcgtgatg cgcagctcgt cgggattgaa
     1261 gccgtcgccg ctccagccga gcgcctgcgt gccgacgcat tgcagcaaga gctcgaattt
     1321 ccgcttgttg agctcggtgc cgtaatactt gaacatggcc gaggcggggc cgggcgtgcc
     1381 ggtcttggcc tcctcggcgg cgcgccggcg cgtcagctgg aaggcctgcg cgtccatctc
     1441 ctgctgcgcg atgcggtcgc gcagcgtcgc gtcggcgagc tttccctcgc gggcgccgac
     1501 ataggatttg ccgatttcgc ccagcgaccg ctcggcgccg cgctggcgtt cgccgatcga
     1561 ggcgatcatc ttgcgctcat gctcgaggag acgcttggcg atggtccagc cctggttgag
     1621 gccgccgacg aggttcgtca tgggcacgcg gacattctcg atgaaggtct cgcagaaggg
     1681 cgaggagccg ctgatgaggc ggatcggccg cgtgcgcacg ccgggatcgg ccatgtcgat
     1741 caggatgaag ctgatgccgt catgcttctt cgccagcgca tcggtgcgga ccaggcagaa
     1801 gatccagtcg gcgtagttgg cgtaagaggt ccagaccttg tggccgttga cgaggaaatg
     1861 ctcgccttgc ggcacggcgc gggtcttgag gccggccagg tccgatccgg cctcgggctc
     1921 gctgtagccc tggcaccagc ggatctcgcc gcgggcgatc ttgggcagat gctcctgctt
     1981 ctgcgcctcg ttgccgaatt cgagcaggac cggccccagc atccagatgc cgaagctgag
     2041 gagcggcgcg cggcagccga ggcgcgccat ctcgtcttcg aggatccgcg tctcggccga
     2101 ggagaggccg ccgccgccat aggccttggg ccagcccggc accgtccacc ccttctcggc
     2161 catgcgctcg agccagatcc tcgagtccgg attcttgaag ctggcgcggc ggccgcccca
     2221 gacgacctcg tcctcgggca tgggggcgcg catgctgcgc ggacaattgg cttcgagcca
     2281 agcatgcgcc tcggcccgga acgcatcgag atcctgcacc gtggtcctcc ctagggtctc
     2341 ccccctatac accagcgctg gcgggccctc aagatcggcg cggttttctg cggcgccgcc
     2401 ggtcttccac agccggggtt gcgccggcat gccatggtgg tgttacagtc ggtttacaag
     2461 cgggtaacag tttgttgcaa gccgccggag aaagtcaccc gtatttcaat ggcttggctg
     2521 gcctctttca catatctgtc gccgttggcc gcatttccgc cgaatccgcg gagggccagg
     2581 gaagacttgg ctccttggac gtcatgcaca gagctatcca caagcatgtc cgacaaccat
     2641 tgcttaacca tggggcgcga tagtcggcga gcatattttc gcagtttatc ggtcaaaccc
     2701 atgtcgtctc cgagcgatgc cgatgacgca gcgccgctgg caccgatcgc gcggctcgtc
     2761 gccacgatca aggccgagcg ggccgcctgc gccgcgcccg ccatcggcgg cgaagagcgc
     2821 gaccgccttg gcgccgagct tcgccagctg atcgccgccc gcgaagagga gcagcgcgcc
     2881 tggcgggatg cgatggacgt gctcaagacc gacctggacg cgacgcgtgc cgccgcacga
     2941 gcggccgaaa cccggcatga gcaggcggcc caggagcacc ggcgcgtcat ggccgatctc
     3001 gagctcaagc acgagcatca gcgctcgatc tggcagctcg accgccggcg cctcgagatc
     3061 accatcgccg gccaggagac ggcgcagcgc gcgcgaagct catggcttgc ggtggcggtg
     3121 gggctgctgg tcgtgggcgt cttcggtgcg gcgctcgtcg ccgaccaccg cgtcggcccg
     3181 cccgcggagg atgcggccgc ggcgcagacg gtttttccgg cgtcgggctc gtcgcgctag
     3241 atcccttgcc gcagtcgttg cggcgtggat ggaaatcgac taaggctgcg tagaatggtc
     3301 tcgaggaggt gccggaaatg agaggcaggc tcctgcttct ggtcgtcgcg gcgtgcctcc
     3361 ccatgacggc ggcgcgcgcc gacatgatgg gcgacgaatt caagcggaat tccagcgtct
     3421 ggcggaacaa cgacctctgc gcgcgccaag cgttcagaca gttcccggac tacacgcccg
     3481 agggcaacgc caagcgcgac catgccatgc agcaatgcct ggcggctacg cacgcgccgc
     3541 cgcgcgactc gacgaacccg aagcccgcag cggcgcccga tcagcagccc caatagctgc
     3601 gatggccgac ggagcaccgc tcctctttcc tctcgccggc cgccgggtgt tcgtggcggg
     3661 ccatcgcggc atggtgggaa gcgcgctggt gcggcggctc gcgcgcgcgg gatgcacggt
     3721 gctcacggcc gagcgcagca tcgatctgcg ccggcaggac gcgaccgagg cgtggctcgg
     3781 cgcggagcgg cccgaggcgg tgttcgtcgc ggcggcgacg gtcggcggca tcctcgccaa
     3841 tgacacgcgc ccggccgatt tcctctacga caacctgatg atcgccggca acgtcatcga
     3901 ggcggcgcgg cggagcggcg tccggaagct gctgctcctc ggctcgtcct gcatttatcc
     3961 gcggttggcg ccgcagccca tgaccgagga cgcgctgctt tccgggccgc tcgagccgac
     4021 gaatcagtgg tatgcggtgg cgaagatcgc gggcctgaag ctctgcgccg cctatcgccg
     4081 gcaatacggg ctcgacttca tttcggcgca gccgaccaat ctctatggcc ccggcgacac
     4141 ctatgatctc cgcgcgagcc atgtcgtgcc ggcgctcatc gccaagacgc accgcgccaa
     4201 ggtgacgggt gccggcgacg tcgagatctg gggcacgggc gcgccgcggc gcgagttcct
     4261 gcatgtggac gaccttgccg acgcgctggt gttcctgatg gagcgctatt cggacgaact
     4321 ccatctcaac atcggctggg gcgaggatgt gagcatccgc gagctcgccg aggtcattgc
     4381 cgaggtcgtg ggcttcaccg gccgcttccg ctacgaccgg acgaagcccg acggcgcgcc
     4441 gcgcaagctc ctcgacgtca cgcgcctgag cgcgctcggc tggcgtcccc gcatcgcctt
     4501 gcgcgaagga ctgacggacg cctatcgctg gttcctcgaa tctctctctc ccctcgcggg
     4561 agagggaggg gcccgacgcg aagcgtcggg agggtgaggg ggctgctgca gctcacccct
     4621 caccctccca gcgctgcgcg ctgggtccct ccctctcccg caaggggaga ggggacatga
     4681 gcgatgtccg cgtcgccgcc gcgcgtcagc gtcgtcattc cgctctacaa tcgcgcacac
     4741 acgatcgcgc gggcgctcgc ctcggttgag cggcaacgct tcggcgattt cgagatcgtc
     4801 gtcgtcgatg acgcgtccag cgatggcggc gccggcacga tcggcgggcg cgatccgaga
     4861 ctccggatct tgcgccacga ggcgaatcgc ggcgcggcgg ctgcgcgcaa caccggcgtc
     4921 gatgccgcgc ggggagaata catcgccttc ctcgactccg atgacgagtg gctgccggag
     4981 aagctcgcga cgcagatcgc cgtgctcgat cggcgcgggc gaggatgcga cgcggtcgtc
     5041 tcggccttta tcctcgtcga cgaaccgagc ggccgccgcc atgcggtgcg tgcgccgttc
     5101 cggcgcgatc cccggcgctc gctgctcttg ggctgcgcgg tgagcccggg atcgacgctg
     5161 gtctgccggc gcgactatct cgtcggggga catctcttcg acgagcggtt gcgccggctc
     5221 gaggattggg actggctgct cacctatgtg cgcgagcgcg atctcgctct catcgacgag
     5281 ccgctcgcca tcatccacgg caatgccggc gcccatgggc ccttcgtgct cgccgctttg
     5341 gagacgatcg aggatcggca tggcggcgcc attgcggcgc tcgggccgag cgctcggcgc
     5401 atgttccgct cgacgctgtt gctcgagcgc gcggcggcgg cttatcgcag ccgagactgg
     5461 cgccgctcgg cttccatgct ctggcgttcg ctgctccttt atccgttccg ctgggatttc
     5521 ttcgcgcgga tgctgcagcg gctcctgccc cggcgtcacc aaggcccctt gccttgacgc
     5581 gggttgccgc gataaggaag tcgcccgaag ccagatcgag gagcggcgct tgtcggtgac
     5641 ggtcgagtcg gaggacattc ggcgcctcta tcgcgcgatg aagcggatcc gccacgtcga
     5701 ggaagagatc gcgcggcttt atcccagcga caagatcaag agccccgtcc atctctcgat
     5761 cggccaggaa tcggtcgccg tcggcatctg cgatccgctt agggcggatg acgtggtctc
     5821 cggcacttat cgcggtcatg cggcctatct cgccaagggc ggctcgctgc cggcgctcat
     5881 ggccgagctc tacggcaagg tcgatggctg cgccgccggc aagggcggct cgatgcatat
     5941 catcgacatg gagcatttca ttctcggcac gtcggcggtc gtcggcagca cgatccccgt
     6001 cgccgtcggc tacgccctcg ccatgaagcg cgagggcagg ggccgcgtcg tcgcctgctt
     6061 cttcggcgac ggcgcgaccg aggaaggtgt gttcgcggag agcctcaatt tcgcggcgct
     6121 gaagcatctc cccgtgctct tcgtctgcga gaacaatcaa tacgctatcc atacgcacac
     6181 cagccggcgc tgggccaccg accagctctg cgcgcgcgtc gccacttacg gcattccggt
     6241 ggagcaggtg gcggatggcg acgtgctggc gatccgcgcg cgcgccggca aagcctatgc
     6301 ggcgatgcgc gacggcggcg gcccggcctt cctcgaatgc cgcacctatc gctggcgcga
     6361 gcatgtcggg ccgggcgagg actacgacgc cggctatcgc cgccgcgccg aactcgaaga
     6421 gtggcagcgc cgcgaccaga tcgcgcggct cgccgccatg ctcgcgccgg ccgaacgcgg
     6481 cgcgatcgat gcggcgatcg aggccgaggt cgcggcggcc gtcgatttcg ccgagaagag
     6541 cccattcccg cccgtcgagc agctcaagac cgatgtcttc gcctgacggt acagagcgcg
     6601 ttctgagcta tgtcgacgca ctgcgcgagg cagtggcgca ggagatggaa cgcgatcccc
     6661 gcgtcttcgt catcggcctc gatgtcgacg accacaaggc gatccagggc tcgacccgcg
     6721 gcctgctcga acgcttcggt ccggagcgcg tcatggggac gccgctctcc gaagaggcga
     6781 tgaccggagt cgccatcggc gcggcgatgg ccggcctgcg cccgatccat gtgcatatcc
     6841 gcatggactt cctgctgctg tgcatgaacc agctcatcaa catcgccgcc aaggcgcgct
     6901 acatgtatgg cggccaggtc gccgtgccgc tggtcgtgcg cagcatgatc ggcaagtcct
     6961 gggggcaggg ggcgcagcac agccaggggc tccattcgct cttcatgcat gtgcccggcc
     7021 tcaaggtggt ggcgcctgcc gatgcctata tggccaaggg ctgcatgatc gcagcgatcc
     7081 gcgacgataa tcccgtgatc ttcgtcgagc atcgcctgct ctatttcacc gacgccgtgg
     7141 tgccggtgga gccttacgcg gtcgagttcg gccgtgcgcg cctctgcacc gaggggagcg
     7201 atatcaccat cgtcggcgtc tccaacatgc tcatcgagtg cctgcgcgcc aacgagctgt
     7261 tgaagagcgc cggcatctct gccgaagtca tcgacccggt ctcgctggtg ccgctcgatc
     7321 tcgcgaccat cagcgcgtcg gtcgcgcgca cggggcggct cctcgtcgtc gacaatgcct
     7381 ggacgacctg cggcgccagc gccgagatca tcgccggcgt cgtcgagcac gccgcggggg
     7441 agcgcatcgt ggcgcggcgc atgggctatg cgcggacgtc ctgtccgacg acgccgtcgc
     7501 tcgagcaggc cttctatcca aacccagcga cgatcgccga gacggcgctc cacatggtgc
     7561 ggccgcacgc gacctggcgg cccgaccccg aagaggcgcg gctcgcctac caggcgcggt
     7621 ttcgcggtcc attttaacac catgcgcaag tcaccgatga ctccaaatac cgtcatcccc
     7681 gcgaaagcgg ggacccaggg gccactcgca cgtcgcttgc ccctgggtcc ccgctttcgc
     7741 ggggatgacg gaaattgggt tgcgccggtg cactagggga cgacgagggg aatgttctac
     7801 gagctcgcag ccagcacctg gggccaggaa gagcacgacg cgatcgcccg cgtggtcaag
     7861 agcggacgct tcaccatggg gcccgaggtg gcggcgttcg aggacgcctt cgcccggtat
     7921 ttcggccaca aatacgcggt gatggtgaat tccggatcct cggccaacct gatcgcggtg
     7981 gcgagcctct tttacaagaa ggagcggccg ctcgagcgcg gcgacgaggt gatcgtgccg
     8041 gcgatctcct ggagcacgac ctatcatccg ctccagcaat atggcttgaa gctgcgcttc
     8101 gtcgacgtcg acctcgcgac gctcaacatc gatgcgagcc agctcgagcg cgcgctgacg
     8161 cgcaagacgc gcatgatcgt cgccgtgagc atcctcggca atcccgcgcc gctcgacgtc
     8221 atgcgccgct tcgccgacaa gcatggcctc tatttcatcg aggacaattg cgagtcgatg
     8281 gacgccgagc tcgcggggcg caagaccggc accttcggcg atgtcgcgac ctacagcagc
     8341 ttcttctcgc accacatctc gaccatggaa ggcgggatgc tggcgaccga cgatcccgag
     8401 ctctgggagc tggcgcggtc gctgcgcgcc cacggctgga cgcgcgacct gccggagggg
     8461 agcaagctct atgagaaagg cgacgatgat ttcttcgagg cctatcgctt catcctgccc
     8521 ggctacaatg tgcggccgct cgagctttcg ggcgccatcg gcatcgagca gctgaagaag
     8581 ctgacgggca tgacggcggc acggcggaag aattgggcga cattcacgtc gcttttcgcc
     8641 ggcgacgagc gcttcgtcat ccagcgcgag aacggcaaga gctcggcctt ctgctttacg
     8701 atcgtgctcg atccgaagcg ccggcccgag cggcgtcgcg tgctggcggc gctcaaggac
     8761 gcggatatcg gctatcgcat catcaccggc ggctgcttca cgcggcacga ggtgatccgg
     8821 cactacgact atacgaccgt cgacgacctg cccaacgcca atctggcgca tgatctcggc
     8881 ttctttgtcg gcaatcaccc tttcgatctg acgccgcaga tcgagcggct gcgcggcgtg
     8941 ctcgatcgcg tctgcaattg aggatcagct atgaccggcg agcgctactc catcctcgtg
     9001 accggcggcg ccggttttat cggctcgatc ctggtgccgg cgctgctcga ggccgggcac
     9061 aaggtgacgg tgctcgacaa tttcatgtac cggcaggaca gcctcaacca cgtctgcgcc
     9121 gatccgaatt tcgcggtcgc gcgcggcgat gcgcgggacg aggcggtgct gcggccgctg
     9181 gttgccaagg ccgatgcggt gatcccgctg gcggcgctgg tgggcgcgcc cttgtgcgac
     9241 cgcgacaaga tcggcgccac ctcgaccaac cgcgatggag tggcgacgct ggtgcggctg
     9301 ctgtcgaaag agcagcgggt gctctacccc aacaccaaca gcggctatgg cgtcggcgac
     9361 aaggggcaat actgcaccga ggagagcccg cttcgcccgg tctcgctcta cggccgcgac
     9421 aaggtggcgg cggaggagat cgtactgggc cacggcaacg ccgtcgtctt ccggctggcg
     9481 acggtgttcg gcatggcgcc gcgcatgcgc atcgacctcc tggtgaatga tttcgtctac
     9541 cgcgccgtca ccgaccgctt catcgtgctc ttcgagggcc atttcaagcg caactacatc
     9601 cacatccgcg acgtcgcccg cgcgttcctg cacggcatcg atcatctcgc ggaaatgcgc
     9661 gggcgcacct acaatgtcgg gctttccgac gccaatctct cgaagatcga gctgtgcgcg
     9721 ctcatcaagc gcttcctgcc gcagctcgtc tatctcgaag cgcccgtcgg cgaggacccc
     9781 gacaagcgcg attacatcgt ctcgaacgcg cgcatcgaag ccacgggctt caagcccaag
     9841 cattcgctcg acctgggcat cgccgagctg atcaagggct acgcgatgat ccgcaacagc
     9901 atttacggca acatgtgagt ggcgctgcgg cgccgcgata tggccgtcaa gcgacggcga
     9961 ggcggcccat gccgacatcc gcggcgagac ggacgtggaa gcgcgcgtag agctcgccgg
    10021 cgatcagcac gcggcgcggc ggggcggctt cggtgaggga gcgctcgagc tgcgccaggt
    10081 cggcgatcac gcgcgtctcg cacggcgcgc ggcagagcca gcgggaccag cgggcgagat
    10141 agcgcgcttg ggcgggggct gccaccagca c
//
LOCUS       DASYNS010000421         9548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4007816, whole genome shotgun sequence.
ACCESSION   DASYNS010000421 DASYNS010000000
VERSION     DASYNS010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 9548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9548
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4007816"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1366
                     /locus_tag="VGP50_13140"
     CDS             65..1366
                     /locus_tag="VGP50_13140"
                     /inference="COORDINATES: protein motif:HMM:NF011656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8028372.1"
                     /translation="MLTAAERKHQVLSVIRVSSGNFLEMYDFFVFGYYAKAIGNAFFP
                     GDSEFFKLMAAFTIFGLGFLMRPVGAIVLGPYIDRHGRRAGLLLTLGLMAIGTLTIAL
                     VPGYATLGLTAPVIVLIGRLIQGFSAGVELGGVSVYLAEIATPGHKGFYVSWQSGSQQ
                     VAVIFVALLGVALSALLPPDTMTAWGWRIPFLIGCLIIPLLFLLRRSMAETAAFATRK
                     QSRNLPTPRQIYQSVARNWAIVLIGMMMVTMTTVSFYFITTYTPTFGREVLKLGDTES
                     LVVTLCVGISNLFWLPVMGAVSDRVGRRPLLILFTSLALVTTYPAMLWLVDAPSFSRL
                     LLVELWLSFLYGSYNGAMVVALTEIMPVEVRTSGFSVAYSLATGIFGGFTPAINTLLI
                     ELTDNKAMPGVWVSCAALLSLGAALLVGNRQQRAALAKPAE"
     gene            1410..2273
                     /locus_tag="VGP50_13145"
     CDS             1410..2273
                     /locus_tag="VGP50_13145"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEV8028373.1"
                     /translation="MSRISYVNGRYLPHRDALVHVEDRGFQFADAVYEVFAIRRGGLV
                     DEALHLARLARSLAELRMAPPMGEAALKLVLREVIRRNRVANGTLYLQVSRGAAPRDF
                     VFPKAARPTLVVTVRPQRRQDPRLLEEGVGVITIPDIRWGRPDIKTVGLLPNALGKQQ
                     ARDTGAFEAWQVDRDGRVTEGTSTNAWIVTADGEVVTRQADHSILNGVTRYGLLQLLG
                     GEGLRLAERPFSVAEAKAAREAFLTSTTNFVLPVVSIDGAPVANGHPGTLTLRLRALY
                     DAYVAAQVAAP"
     gene            2453..2701
                     /gene="hfq"
                     /locus_tag="VGP50_13150"
     CDS             2453..2701
                     /gene="hfq"
                     /locus_tag="VGP50_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEV8028374.1"
                     /translation="MSSEKSQNVQDVFLNHVRKNKTPVTVFLVNGVKLQGIITWFDNF
                     CVLLRRDAHSQLVYKHAISTVMPAQPIQLFEPKEGDEA"
     gene            2774..4030
                     /gene="hflX"
                     /locus_tag="VGP50_13155"
     CDS             2774..4030
                     /gene="hflX"
                     /locus_tag="VGP50_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002731149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV8028375.1"
                     /translation="MLKVAPSGSGDGARSPEARLQEAVGLATAINLEVVHAEIVRVVR
                     WRPATLIGSGAVEKCAGTVEEKAIAVAVIDGTVSPVQQRNLERALKCKVIDRTGLILE
                     IFGARARTREGQLQVELAALSYQRSRLVRSWTHLERQRGGFGFLGGPGESQLEIDRRL
                     IGERIAKLKRELEEVKRTRGLHREARKRVPYPVVALVGYTNAGKSTLFNRLTRSAVVA
                     EDMLFATLDPAMRRLPLPSGRQAILSDTVGFVSELPTHLVAAFRATLEEVTEADLIVH
                     VRDIHHPDSEAQKSDVHAVLGDLGLGETVEHGLIEALNKIDLLEPEQREVLLRQAQRN
                     RDVVSISAVTGEGCGALMRLLDYRVEGDRQLVHLDVPVSDGAALAWLYAHGEVMTRRD
                     DEEEAHVAVRLSEADLGRFRSRRLAH"
     gene            complement(4264..5073)
                     /locus_tag="VGP50_13160"
     CDS             complement(4264..5073)
                     /locus_tag="VGP50_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEV8028376.1"
                     /translation="MAATQAQKAEQFRALHARPGAFVIPNPWDVGSTRILAGLGFEAL
                     ATTSGGFAVSMGKRDGAMSRDDVLAHCRSIAGATDLPVSADLENCFGDDPRAVAETIR
                     LAAETGLVGGSVEDWSGRAIYDFDHAVERVAAAVKAARALPFPFTLTARAENFLRGRR
                     DLDDTIRRLQAFEKAGADVLYAPFLPDLAAIKAVCAALKKPVNVLAGRPPFTVAELAA
                     SGVKRVSLGSLLANVATGALLRAAREIKAQGSFTFAESAASTKEVNELMAS"
     gene            complement(5256..6458)
                     /locus_tag="VGP50_13165"
     CDS             complement(5256..6458)
                     /locus_tag="VGP50_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8028377.1"
                     /translation="MRLTGFAATLLALAASAAPAGAQVKVGVLSDMASLYADITGAGT
                     VAAARMAAADAGPVLGAPVEIVSGDHQNKPDIASALARRWYDADGVDMITDGGSSAVA
                     LAVEEVSREKHKLVLFSGPASSDITGKNCSPYAAHWTYDTYALAHVTGSAIVKAGGDT
                     WFFLGADYAFGHALERDTAAVVEANGGKVLAAVYAPLNSADFSSFLLQAQESKAKVIG
                     LANAGGDTINAIKQGAEFGVAEGGQSFAALLMFITDVHSLGLKTAQGLKFTTAFYWDQ
                     NDGTRAFAKRFFAEIGHMPTMVQAGVYSATHHYLAAIKAAGTKDPEAVMAKMREMPIN
                     DFMTHDGKLRVDGRVIRDMYLLQVKKPEESKAPWDYARVVETVPGEQAFRPLDQGGCP
                     LVTAAAGK"
     gene            complement(6638..6937)
                     /locus_tag="VGP50_13170"
     CDS             complement(6638..6937)
                     /locus_tag="VGP50_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028378.1"
                     /translation="MSIATGISSSQSAYQLYQQQLQQQSQQLEAQQLQQQQQSQQTEA
                     QPHHHHQKPDGAGAAGQTQPSAGSAASGASTLPKAASASSNSSATGTGSSLDLFA"
     gene            7190..8056
                     /locus_tag="VGP50_13175"
     CDS             7190..8056
                     /locus_tag="VGP50_13175"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8028379.1"
                     /translation="MKLAFLGLGVMGYPMAGHLAKAGHQVTVYNRTPAKAEAWAKDHG
                     GSHRPSPMEAAKGADIVFSCVGNDDDIRAVALGPEGAFKGMEPGAIFADHTTASADIA
                     RELAARAVERGLGFLDAPVSGGQAGAVNGKLTIMVGGEPQAFERAAPVMAHYGRAVTL
                     MGPAGSGQLTKMVNQICIAGLVEALAEGLNFAQRAGLDTRQVVEVIAKGAAQSWQMEN
                     RAHTMVEGKFDFGFAVDWMRKDLAICLAEARRNKAALPVTALVDQFYARLQERGGRRW
                     DTSSLIDLLANP"
     gene            8399..9145
                     /locus_tag="VGP50_13180"
     CDS             8399..9145
                     /locus_tag="VGP50_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8028380.1"
                     /translation="MDQERELKGRVALVTGASRNIGRAIALDLAAAGAAVVVNTRQAR
                     AAADAVAREIADAGGQAMSVVADVTDETAVAAMIDAVAQRFGRLDVLVNNAATRPARH
                     IEEMTLAEFRAVVGLILEGAFLCARAALPLLKQSDAASIISIGGMTGHVGGGDRVHVA
                     AGKAGLVGLTKALARELAPHGITANCVVPGLIDTARNAVPSAHAANPSLLGRLGQMGE
                     IAALVRYLAGPAARFITGQAIHVNGGAYMP"
BASE COUNT         1502 a         3212 c         3205 g         1629 t
ORIGIN      
        1 gctcctcatc ctgagcttgt cgaaggacgc acatcggaat tgcagcgcgt aaagggagga
       61 tgacgtgctg acggccgctg aacgcaagca ccaggtcctg tcggtgatcc gcgtctccag
      121 cggcaatttc ctggagatgt atgacttctt cgtcttcggt tactacgcga aggcgatcgg
      181 caacgccttc ttccccggcg acagcgagtt cttcaagctg atggcggcct tcaccatctt
      241 cggcctcggc tttctcatgc gccccgtggg tgcgatcgtg cttggccctt atatcgaccg
      301 tcacggccgc cgcgccggac tcttgctcac cctcggcctc atggccatcg gcacgctcac
      361 catcgcgctg gtgccgggat atgcgacgct ggggttgacc gcgcccgtca tcgtcctcat
      421 cggccgcttg atccagggtt tctcggccgg cgtcgagctg ggcggcgtct ccgtctatct
      481 cgccgagatc gcgacgccgg gacacaaggg cttctatgtg agctggcagt cgggcagcca
      541 gcaggtcgcg gtcatcttcg tcgctctcct cggcgtcgcc ttgagcgcgc tgctgccgcc
      601 cgacaccatg acggcctggg gctggcgcat tcccttcctg atcggctgcc tcatcattcc
      661 gctgctcttc ctgctccgcc gctcgatggc ggagaccgcg gcgttcgcga cgcgaaagca
      721 aagccgcaat cttccgacgc cgcgccagat ctatcagtcg gttgcgcgca actgggcgat
      781 cgtgctcatc ggcatgatga tggtgacgat gaccactgtc tccttctatt tcatcaccac
      841 ctacaccccg actttcgggc gcgaggtgct gaaactcggc gacaccgaga gcctcgtcgt
      901 cacgctctgc gtcggcatct ccaatctctt ctggctgccg gtgatgggcg cggtctccga
      961 ccgcgtcggc cgccgcccgc tgctgatcct cttcaccagc ctcgccttgg tcacgaccta
     1021 tccggcgatg ctgtggctgg tggacgcgcc gtctttctcg cgattgctgc tggtggagct
     1081 gtggctctcg ttcctctacg gcagctacaa cggcgcgatg gtggtggcgc tgaccgagat
     1141 catgccggtc gaagtgcgca cctccggctt ctcggtcgcc tacagcctcg ccaccggaat
     1201 cttcggtgga ttcacgccgg cgatcaacac cctgctgatc gagctcaccg acaacaaggc
     1261 gatgccgggc gtatgggtct cctgcgccgc gctgctgagc ctcggggccg cgctccttgt
     1321 cggcaaccgc cagcagcgcg cggcgctcgc gaagccggcg gagtagccgc ctcgtctttt
     1381 cgcgcggtca cccctatagt cggcgcccca tgtcgcgcat ctcctatgtc aatggccgct
     1441 acctgccgca ccgcgacgcg ctggtgcatg tcgaggatcg cggcttccag ttcgcggacg
     1501 ccgtctacga ggtcttcgcc atccggcgcg gcgggctcgt cgacgaggcg ctgcatctgg
     1561 cgcgcctcgc ccgctcgctg gccgagctca gaatggcccc gcccatgggc gaggcggcgc
     1621 tcaagctggt gctgcgcgag gttatccggc gtaaccgcgt cgcgaacggc accctttacc
     1681 tgcaggtgag ccgcggcgcg gcgccccgcg acttcgtctt ccccaaagcg gcgcggccga
     1741 cgctcgtcgt cacggtgcgc ccgcagcgcc gccaggatcc gcggctgctg gaagagggcg
     1801 tcggcgtcat cacgatcccc gacatccgct gggggcgccc cgacatcaag accgtgggcc
     1861 tgttgcccaa tgcgctgggc aaacagcagg cgcgcgacac gggcgccttt gaggcctggc
     1921 aggtcgatcg cgatggccgt gtcaccgagg gcacctcgac caatgcctgg atcgtcaccg
     1981 cggacggcga ggtggtcacc cgccaggccg atcacagcat cctcaacggc gtgacgcgct
     2041 acggtctgct ccagctcctg gggggcgagg gcctgcgcct tgccgagcgg cccttcagcg
     2101 tcgccgaggc gaaagccgcg cgcgaggcct tcctcaccag caccaccaac ttcgtcctgc
     2161 cggtggtgtc gatcgacggc gctcccgtgg ccaacggcca tccggggacg ctgaccctca
     2221 ggctgcgcgc gctctatgac gcctatgtgg cggcgcaggt ggcggcgccg tgaccggcgc
     2281 tccgctcggg cgacgacgga aggctgcccg gcccggcaca tagggttgtg atttccctcg
     2341 gccgaccgat ttgttacgat cctgccccga gtcgcatgag accgccgccc gctccgggaa
     2401 gcggatttca aggctccgac cgacccaaag aacggaataa ggggcaatac ccatgtcgtc
     2461 ggaaaaatca caaaatgtgc aggacgtctt cctcaaccac gtacgcaaga acaagacgcc
     2521 ggtgacggtg ttcctggtca atggcgtgaa actccagggc atcatcacct ggttcgacaa
     2581 tttctgcgtc ctgttgcggc gggacgcgca ttcgcagctc gtctacaagc acgcgatctc
     2641 gaccgtcatg ccggcgcagc caatccagct cttcgaaccc aaggagggcg atgaagccta
     2701 ggaatctctc ttcttaatgg cgccccggga cgagagcagc ctctcgacgg gccgctgcct
     2761 ggtgctccat ccgatgctga aggtcgcgcc ctcgggcagc ggcgacggcg cgcgctcgcc
     2821 cgaggcgcgg ctccaggagg cggtcggcct ggccaccgcc atcaacctcg aggtcgtcca
     2881 tgccgagatc gtgcgcgtcg tccgctggcg gcccgcgacg ctgatcggca gcggcgcggt
     2941 cgagaaatgc gcggggaccg tcgaggagaa ggcgattgcc gtcgcggtca tcgacggcac
     3001 ggtgagcccg gtgcagcagc gcaacctcga gcgcgccttg aagtgcaagg tcatcgaccg
     3061 cacggggctg atcctcgaga ttttcggcgc ccgcgcccgc acgcgcgagg gtcagcttca
     3121 ggtcgaactc gccgcgctca gctatcagcg ctcgcgcctc gtgcgatcct ggacgcatct
     3181 cgagcgccag cgcggcggct tcggcttcct cggcggcccc ggggagagcc agctcgagat
     3241 cgaccgccgc ctcatcggcg agcgcatcgc caagctcaag cgcgagctgg aagaggtgaa
     3301 gcgcacccgc gggcttcatc gcgaagcgcg caagcgcgtg ccttatcccg tggtcgcgct
     3361 ggtgggctac accaatgccg gcaagtcgac gctgttcaac cgcctgacgc gctcagccgt
     3421 cgtcgccgag gacatgctct tcgccaccct cgatccggcg atgcgccgcc tgccgctgcc
     3481 atcggggcgc caggcgatcc tgtcggacac ggtgggcttc gtctcggagc tgccgacgca
     3541 tctcgtcgcc gccttccgcg cgacgctcga ggaggtcacg gaggccgacc tcatcgtcca
     3601 tgtgcgcgac atccatcatc ccgacagcga ggcccagaag agcgacgtcc acgccgtgct
     3661 gggcgacctt ggcctcggcg agacggtgga gcatgggctc atcgaggcgc tcaacaagat
     3721 cgatctgctc gagcccgagc agcgcgaggt gctgttgcgg caggcacaac gcaaccgcga
     3781 cgtcgtctcg atttccgccg tcaccggcga aggctgcggc gcgctgatga ggctgctcga
     3841 ctaccgcgtc gaaggcgacc gccagctcgt ccatctcgac gtgcccgtga gcgacggcgc
     3901 ggcgctcgcc tggctctatg cccatggcga agtgatgacg cggcgcgacg acgaggagga
     3961 ggcgcatgtc gcggtgcgac tgtcggaagc cgacctcggc cgctttagga gccggcgcct
     4021 cgcgcattaa gaggggtcac cacagaggca cagagacaca gagacacaga gacacagagg
     4081 aaaaaagaag attcaccgct gagacggtga gaagattttt tggcgcgcga agcgcgccat
     4141 tttccttcct cactgtctca ccgcctcacc ggtcaatctt ttttcgaagc cacgagcgag
     4201 gggcgtgccg gactttttcg gcgcctctgt gtctctgcgc ctctgtggtc aatctttctt
     4261 tcttcatgag gccatgagct cgttcacctc tttcgtcgag gcggcgctct cggcgaaggt
     4321 gaagctgcct tgcgccttga tctcgcgcgc cgcgcgcagc aacgcgccgg tggcgacgtt
     4381 ggccagaagg ctgccgaggc tgacgcgctt gacgccggaa gcggccagct cggccacggt
     4441 gaagggcggc cgtcccgcga gcacattgac cggtttcttg agtgcggcgc acaccgcctt
     4501 gatggcggcg aggtcgggca ggaagggcgc atacagcacg tcggcgccgg ccttctcgaa
     4561 ggcctggaga cggcggatgg tgtcgtcgag atcgcgccgt ccgcgcagga aattctcggc
     4621 gcgggccgtg agcgtaaacg ggaaggggag ggcgcgcgcc gctttgaccg cggcggcgac
     4681 gcgctcgacc gcgtgatcga aatcatagat cgcgcggccg ctccagtctt cgaccgagcc
     4741 gcccacgagg ccggtctcgg cggcgagccg gatcgtctcc gcgaccgccc tcgggtcgtc
     4801 gccgaagcaa ttctcgagat cggccgagac gggcagatcg gtcgccccgg cgatgctgcg
     4861 gcaatgggca agcacgtcat cacggctcat ggcgccgtcg cgcttgccca tcgagacggc
     4921 gaagccgccg ctcgtcgtcg ccagagcctc gaagccgaga ccggcgagga tgcgggtgct
     4981 gccgacatcc cagggattgg gaatgacgaa ggctccgggc cgtgcatgga gcgcgcgaaa
     5041 ctgctcggcc ttctgcgctt gtgtcgctgc catcaaacct cctcctcgtc cgatcgcttt
     5101 cagcccgaac cgcgactctc gccccgaatt cctgggcggc gcgagagctt cctgcgcagc
     5161 tcaggcctgc tcggctttca tagattgccc cggagcgcgc gctcgtggct ggcggtaatc
     5221 ggctgcgaat atcgagattt cgcggccgtt gcggtctact tgcccgcggc cgccgtcacc
     5281 agcgggcagc cgccctggtc gagcggacgg aaagcctgct cgcccggcac cgtctcgacg
     5341 acgcgcgcat agtcccaggg cgccttcgat tcctccggct tcttcacctg cagcagatac
     5401 atgtcgcgga tcacgcggcc gtcgacgcgc agcttgccgt cgtgagtcat aaaatcgttg
     5461 atcggcatct cccgcatctt cgccatcacc gcctcgggat ccttggtgcc ggccgccttg
     5521 atcgccgcga gatagtgatg cgtcgcgctg tagacgccgg cctgcaccat ggtcggcatg
     5581 tggccgatct cggcgaagaa acgcttcgcg aaggcacggg tgccgtcgtt ctggtcccag
     5641 tagaaggccg tggtgaattt gaggccctgc gcggtcttga ggccgaggct gtgcacgtcg
     5701 gtgatgaaca tgaggagcgc ggcaaaactc tgcccgccct cggcaacgcc gaactcggcg
     5761 ccctgcttga tggcgttgat ggtgtcgccg ccggcgttgg cgaggccgat gaccttggcc
     5821 ttcgactctt gcgcctgcag caggaaggac gagaagtcgg cgctgttgag cggcgcatag
     5881 accgcggcca gcaccttgcc gccattcgcc tcgaccaccg ccgcggtgtc gcgctcgagg
     5941 gcgtgaccga aggcgtagtc ggcgccgagg aagaaccagg tgtcgccgcc ggccttcacg
     6001 atcgccgagc ccgtgacatg ggcaagcgca taggtgtcgt aggtccaatg cgcggcatag
     6061 ggcgagcagt tcttgccggt gatgtccgag ctcgcggggc cgctgaagag cacgagcttg
     6121 tgcttctcgc gcgacacctc ctcgacggcc agcgccaccg ccgaggagcc gccatcagtg
     6181 atcatgtcga cgccgtccgc gtcgtaccag cgccgcgcca gcgccgaggc gatgtcgggc
     6241 ttgttctgat ggtcgccgga aacgatctcg acgggtgcgc cgagcaccgg gcctgcatcc
     6301 gcggcggcca tgcgcgccgc cgccacggtg ccggcgccgg tgatgtcggc atagagcgag
     6361 gccatgtcgc tgagcacgcc gaccttgact tgcgcgccgg cgggagcagc gcttgcggca
     6421 agcgccagca gggtggccgc gaaaccggtg agacgcattt ttcctccctc gccgcgccgt
     6481 tgtcgcgcgc ggtcgcgcga tcataccgcc gcgcgcagct ccggaacagg gtcgggaaaa
     6541 agggcgaagg cggcatccgg cgccgccatt ctggcggtgc cggacgagct cgtttcacga
     6601 cttcgtgtcg tgagctcgcg caaggatggt ttcttgatca ggcgaagaga tcgaggctgc
     6661 tcccagtccc ggtcgcgctc gaattgctcg aggcggaagc cgctttcggc aaggtcgaag
     6721 cgccggaagc ggccgagccg gccgatggct gcgtctgacc ggcggcgcca gcaccatccg
     6781 gcttctgatg gtgatggtgg ggctgagctt ccgtctgctg gctctgctgc tgctgctgaa
     6841 gttgctgagc ctccagctgc tggctctgct gctggagttg ctgctggtag agctggtagg
     6901 cggattgcga actcgaaatg cccgtagcga tgctcatgat atctccatcg tggttgatgc
     6961 agtgggccat ggatcgcgcg aagtttggcc caagcggttc tggagcaatt ctcgtgcccg
     7021 cgcggggcgg ccgatttccg ccgccgagcc gcgagaaacg tcggagagcc ggcaatattt
     7081 tccttgccgg ccgcaatttt tgccgagatc gggcaagccg gcgcgccgat tgtcagccgc
     7141 ggcggccgcg cggcatagtg ccggcgcgaa acagggagga acggtcgcga tgaagctggc
     7201 attcctgggc ttgggtgtca tgggctatcc gatggcggga catctcgcca aggcgggaca
     7261 ccaggtgacg gtctacaacc gcacgccggc gaaggcggaa gcctgggcca aggatcatgg
     7321 cgggagccac cgccccagcc cgatggaagc ggcgaaaggg gcggacatcg tcttcagctg
     7381 cgtcggcaat gatgacgata tccgcgcggt tgcgctcggt cccgagggcg cgttcaaggg
     7441 catggagccg ggcgccatct tcgccgacca tacgaccgcc tcggccgaca tcgcccgcga
     7501 gctcgccgcg cgcgccgtgg agagggggct gggcttcctc gacgcgccgg tctcgggcgg
     7561 acaggcgggc gcggtcaacg gcaagctcac gatcatggtg ggcggggagc ctcaggcgtt
     7621 cgagcgcgcc gcgccggtga tggcgcatta cggccgcgcc gtcaccttga tgggcccggc
     7681 gggcagcggc cagctcacca agatggtcaa ccagatctgc atcgccggcc tggtggaggc
     7741 actcgccgaa ggcctcaact tcgcgcagcg cgccggcctc gacaccaggc aggtcgtcga
     7801 ggtgatcgcc aagggcgcag cgcaatcgtg gcagatggag aaccgtgcgc acaccatggt
     7861 cgagggaaag ttcgacttcg gctttgccgt cgactggatg cgcaaggatc tcgccatctg
     7921 tctcgccgaa gcgcggcgca acaaggcggc gctgccggtg acggcgctgg tcgatcagtt
     7981 ctatgcgcga ctccaggagc gcggtggccg gcgctgggac acgtcgagtc tcatcgatct
     8041 gctcgccaat ccgtgatcgg cggcccgagt cgcgacctcg cgcgatcaca ttcgcgcgac
     8101 gaaaatcgcg agtccgccag cgcggcggct gcgacgaatc accgggaatt gtcggggcag
     8161 gggatggtcg gcgctgtcgc cgcctatgac tcatggtaga gtgcgacagt tgcgctgatg
     8221 atgacaaacg aatcgccgcg ttccggcaat cgccccgagc gatggctgtc ctggctgacc
     8281 ctggtggcag gcgccgtcat gtgcgccgtc gccgtcctgt tgtcctgaca caggcctccg
     8341 caattcccag gctcggcgcg cgggcatgcg cgcgcgtggc ggctctctcg aggatcgcgt
     8401 ggatcaggaa cgagaactga agggccgcgt cgcgctcgtc acgggcgcgt cgcgcaatat
     8461 cggccgcgcc atcgcgctcg atctcgcggc cgccggcgct gccgtcgtcg tcaacacgcg
     8521 ccaggcgcgc gcagcggccg acgccgtcgc gcgcgagatc gcggatgccg gtggccaggc
     8581 gatgagtgtt gtcgccgacg tcacggacga gacggcggtc gcggccatga tcgacgcggt
     8641 cgcgcagcgc ttcggccgcc tcgacgtcct cgtcaacaac gccgcgactc gtcccgcgcg
     8701 acacatcgaa gagatgacgc tcgcagagtt tcgcgccgtg gtgggcctca tcctcgaagg
     8761 cgctttcttg tgcgcgcgtg ccgcgctgcc gctgttgaag cagagcgacg ccgcttcgat
     8821 catcagcatc ggcggcatga ccggccatgt cggcggcggc gaccgcgtcc atgtcgctgc
     8881 cggcaaggcc ggactcgtcg gcctgacgaa agcgctggcg cgcgagctcg cgccgcatgg
     8941 catcaccgcc aattgcgtcg tgcccgggct gatcgacacg gcgcgcaatg cagtgccatc
     9001 ggctcatgcc gcgaatccgt cgctgctcgg ccgactgggg cagatgggcg agatcgccgc
     9061 gctggtgcgc tacctcgcgg gtcccgcggc gcgcttcatc acggggcagg ccattcacgt
     9121 gaatggcggc gcttatatgc cgtagtcgtg atcgaggagt cgcaaacggc gcggctcccg
     9181 ctccggcaaa atgacaggga attaacttgg tcgaggggcg gtcttcgtcc ctaatgacgt
     9241 cagcggctct gccgcatccc gactgtccaa ccccctttgg cggacgggac ccctgggtcg
     9301 ccccgtctgc gcttcctcct ccaaagtgca ggcggggcat tccatttcag cgcagccgcc
     9361 aaaacgcaac gcagcgaacg cggtgtgtcg tcgcacgacg ataactcaca cccctctccc
     9421 ccgaagggga gagggggaaa gcaacagatc gcttgtcgct agaagctcga accgccgcta
     9481 gagccaccgc cccagctgct gccaggcggc ttcggcggcg tcgacgacga gctgtcggac
     9541 gacggctt
//
LOCUS       DASYNS010000422         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4946020, whole genome shotgun sequence.
ACCESSION   DASYNS010000422 DASYNS010000000
VERSION     DASYNS010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4946020"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /gene="boxC"
                     /locus_tag="VGP50_13185"
     CDS             <1..1543
                     /gene="boxC"
                     /locus_tag="VGP50_13185"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HEV8028381.1"
                     /translation="KLKLNSYDLGVDIELHDALNRVRFEHPEVKTVVLTSGKERMFCA
                     GANIYMLGTSTHAWKVNFCKFTNETRNGIEESSASSGLKFLAALNGTCAGGGYEVALA
                     CDEIVMVDDRSSTVSLPEVPLLGVLPGTGGLTRLVDKRKVRRDLADVFCTTAEGVRAD
                     RARQWKLIDDAAPPARFAALVQERARALAQRSDRPGTGPGVALPPLERKIDDAGYHYR
                     WVDVRFDRAQRNATLTVTGPKEPPPQSGDAMLAAGAQWWPLAMARELDDAILMLRVNE
                     LELGTLVVKTRGAPALMLASDAAMLAQRQHWFVRETLSFLRRSLARIDVSARSVFALI
                     DEGSCFAGTLFELALAADRSYMLAVPESSDGRPNAAAPRIALSDVNFGAFPMVNGLSR
                     LVTRFCGDPAPVAALPAHRGEPLDAAQAMALGLVTFTPDELDWEDEIRVALEERASLS
                     PDALSGLEANLRFAGPETMETRIFGRLSAWQNWIFNRPNAVGEKGALKLFGSGSKPNF
                     NWERV"
     gene            1548..>2099
                     /locus_tag="VGP50_13190"
     CDS             1548..>2099
                     /locus_tag="VGP50_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEV8028382.1"
                     /translation="MTIDYQERIPNNVGLAENRTLQRALEHWQPRFLDWWREMGPSDF
                     LDSDVYLRTATSVDAKGWASYGTVKMPEYRWGIFLADAVPDRTIGFGDEFGKPAWQQV
                     PGEHRSTLRRLIVTQGDTEPASVEQQRLLGHTCPSLYDLRNLFQVNVEEGRHLWAMVY
                     LLHAYFGRDGREEAEGLLMRHSGD"
BASE COUNT          326 a          752 c          687 g          334 t
ORIGIN      
        1 caagctcaag ctcaattcct atgatttggg cgtcgatatc gagctgcacg acgcgctgaa
       61 ccgcgtccgc ttcgagcacc ccgaggtcaa gaccgtggtc ctcaccagcg gcaaggagcg
      121 catgttctgc gccggcgcga atatttacat gctcggcacc tcgacccatg cctggaaggt
      181 caatttctgc aaattcacca acgagacgcg gaacgggatc gaggaatcga gcgcgagctc
      241 cggcctcaaa ttcctcgccg cgctcaacgg cacctgcgcc ggcggcggct atgaggtcgc
      301 gctcgcctgc gacgagatcg tcatggtgga cgaccgctcc tcgacggtga gcttgcccga
      361 agtgccgctg ctcggcgtgc tgcccggcac cggcggcctc acgcgcctcg tcgacaagcg
      421 caaggtcagg cgcgatctcg ccgacgtctt ctgcacgacg gccgaaggcg tgcgcgccga
      481 ccgcgccagg caatggaagc tcatcgatga cgcggcgccg cccgcgcgct tcgccgcgct
      541 ggtgcaggag cgcgcacgag cgctggccca gcggagcgac cggcccggca ctggccccgg
      601 cgtcgcgctt ccgcccttgg agcgcaagat cgacgacgcc ggctatcact atcgctgggt
      661 cgatgtgcgc ttcgaccgcg cgcagcgcaa cgccacgctc accgtcaccg gtcccaaaga
      721 gccgccgccg caaagcggcg acgccatgct cgcggcgggc gcgcaatggt ggccgctcgc
      781 catggcgcgc gagctcgacg atgccatcct catgctgcgc gtcaacgagc tcgaactcgg
      841 cacgctggtg gtgaagacgc gcggggcccc cgcgctcatg ctggcgagcg acgcagcgat
      901 gctggcgcag cgccagcact ggttcgtgcg cgagactctg agcttcctgc gccgcagcct
      961 ggcgcgcatc gacgtgtcgg cgcgcagcgt cttcgccctc atcgacgagg gctcgtgctt
     1021 tgccggcacg ctcttcgagc tcgcgctcgc cgccgaccgc tcctacatgc tcgcggtgcc
     1081 ggagagcagc gacggccggc cgaacgcggc ggcgccccgc atcgcgcttt cggacgtgaa
     1141 tttcggtgcc ttcccgatgg tcaacgggct gtcgcgcctc gtcacccgct tctgcggcga
     1201 cccggcgccc gtggccgcgt tgcccgcgca tcgtggggag ccgctcgatg cggcccaggc
     1261 gatggcgctc ggcctcgtca ccttcacgcc cgacgagctc gactgggagg acgagatccg
     1321 cgtcgcgctc gaggagcgcg cgtcgctctc gcccgatgcc ttgtccggcc tcgaggccaa
     1381 tctccgcttc gccgggcccg agacgatgga gacgcgcatc ttcggccgcc tctcggcctg
     1441 gcagaactgg atcttcaatc ggcccaatgc ggtgggcgag aagggcgcgc tcaagctctt
     1501 cggcagcggt tccaagccga acttcaattg ggaaagggtc tgaccccatg accatcgatt
     1561 atcaggagcg gatcccgaac aatgtcggtc tcgccgagaa ccgcacgctg cagcgcgcgc
     1621 tcgagcattg gcagccgcgc ttcctcgact ggtggcgcga gatggggccg agcgatttcc
     1681 tcgactccga cgtctatctg cgcacggcca cctcggtcga cgccaagggt tgggcgagct
     1741 acggcaccgt caagatgccg gagtatcgct ggggcatctt cctcgccgat gccgtgcccg
     1801 accgcaccat cggcttcggc gacgaattcg gcaagcccgc ctggcagcag gtgccgggcg
     1861 agcaccgctc gacgctgcgg cgcctcatcg tgacgcaagg cgacaccgag cccgcctcgg
     1921 tcgagcagca gcggctcctc ggccatacct gcccctcgct ctacgatctg cgcaacctct
     1981 tccaggtcaa tgtcgaggag ggccgccatc tctgggcgat ggtctatctg ctgcacgctt
     2041 atttcggccg cgacggccgc gaagaggccg aagggctgct gatgcgccat tcgggcgac
//
LOCUS       DASYNS010000423         1860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4479165, whole genome shotgun sequence.
ACCESSION   DASYNS010000423 DASYNS010000000
VERSION     DASYNS010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4479165"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1786)
                     /locus_tag="VGP50_13195"
     CDS             complement(<1..1786)
                     /locus_tag="VGP50_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEV8028383.1"
                     /translation="MMGLNRAKAAFCHDVVMAAASFWLSLYLRIGDDVFDLPPRLLVS
                     YDAAFALIAGAVFWGTGLYRGIWRYASLPDVVALLRAVTIVMLIFFPAMFVATRLEAL
                     PRSLVGINWLVLAALLGGPRFSYRLFKDRSFGRLLERGSARNGAIPVLLIGARDGAAM
                     FIREMASDPARLYRVLGIISNTESRVGRALAGVPVLGTIDDLERVVATLDHRGERPQR
                     LILCAQTFEGATVRRLLDIADALTIPLARLPRITEFKKTLPEDRPAIEPVAIEDLLGR
                     PQAVLDRAGMRALVAGRRVLVTGAGGTIGAELVRQIAAYGPARLVLLDSGEYALYSID
                     RELEEQWKTLPRAALIGDVRDQARVDEVIAAERPELVFHAAALKHVPMVEANPIEGVL
                     TNVVGTRHVAESCRAHGVLAMVMISTDKAVNPTSVMGATKRLAESICQALDLVEKRKP
                     GGTRFVTVRFGNVLGSTGSVVPLFQRQLAAGGPLTVTHPDIERFFMTVREAVELVLAA
                     SALALKEGREDRGKIFVLDMGEPVRIVDLARQMIRLAGLKPDKDVTIEFVGLRPGEKL
                     YEEMFHAKEPLLPTEAAGIRLAAPRVIDH"
BASE COUNT          319 a          620 c          663 g          258 t
ORIGIN      
        1 catggtcgat gacccgtggc gcggcgagcc ggatgcccgc cgcctcggtc ggcagcagcg
       61 gctctttggc gtggaacatc tcctcataga gcttctcgcc ggggcgcagt ccgacgaact
      121 cgatcgtcac gtccttgtcg ggcttcaggc cggcgagacg gatcatctgg cgcgccagat
      181 cgacgatgcg cacgggctcg cccatgtcga gcacgaagat cttgccgcga tcctcgcgcc
      241 cctccttgag ggcgagcgcg gaagcggcga gcaccagctc caccgcctcg cgcaccgtca
      301 tgaagaagcg ctcgatgtcg gggtgtgtca cggtgagcgg accgcccgcc gcgagctggc
      361 gctggaagag cggcaccacc gagccggtcg agcccagcac attgccgaag cgcacggtga
      421 cgaagcgcgt gccgccgggc ttgcgcttct cgacgagatc gagcgcctgg cagatgcttt
      481 cggcaagccg cttcgtggcg cccatgacac tggtcgggtt caccgccttg tcggtcgaga
      541 tcatcaccat cgcgagcacg ccatgcgcgc gacaggattc ggcgacatgg cgcgtgccga
      601 cgacattggt gagtacccct tcgatggggt tggcctcgac catcggcaca tgcttcaagg
      661 cggctgcatg gaagacgagc tccggccgct ccgccgcgat cacctcgtcg acgcgcgcct
      721 ggtcgcgcac gtcgccgatg agcgccgcgc gcggcagcgt cttccactgc tcctcgagct
      781 cgcggtcgat cgaatagagg gcatattcgc cactgtcgag cagcacgagc cgtgccggac
      841 cataggccgc gatctgccgc accagctccg cgccgatggt gccgccggcg ccggtcacca
      901 gcacgcggcg tccggcgacg agcgcgcgca taccggcgcg gtcgagcacg gcctgcggcc
      961 gccccagcag atcctcgatg gcgaccggct cgattgccgg ccggtcctcg gggagtgtct
     1021 tcttgaattc ggtgatacgc ggcaggcgcg ccagcgggat ggtgagcgcg tcggctatgt
     1081 cgagcagccg gcgtacggtc gcgccttcga aggtctgggc gcagaggatg aggcgctgcg
     1141 gccgctcgcc gcggtggtcg agcgtcgcca ccacgcgctc gagatcatcg atcgtgccga
     1201 gcaccggcac gccggcgagc gcgcggccga cgcgggattc ggtgttggag atgatgccga
     1261 ggacgcggta gagccgcgcc ggatcgctgg ccatctcgcg gatgaacatg gcggcgccgt
     1321 cgcgcgcgcc gatcaacagc acggggatcg cgccattgcg cgcgctgccg cgctcgagaa
     1381 gccggccgaa gctccggtcc ttgaacaggc ggtagctgaa gcgcgggccg ccgagaaggg
     1441 cggcgagcac cagccagttg atgccgacga gcgagcgcgg caaggcctcg aggcgggtgg
     1501 cgacgaacat cgccgggaag aagatcagca tcacgatggt gacggcacgc agcagcgcca
     1561 cgacatcggg cagcgaggca tagcgccaga tgccgcgata gagcccggtg ccccagaaga
     1621 ccgcgccggc gatcagggcg aaagcagcgt cgtagctgac gagcagcctt ggcggcaggt
     1681 cgaagacgtc gtcgccgatg cgcaagtaga gcgacagcca gaacgacgcc gcggccatca
     1741 ccacgtcatg gcagaacgcg gctttggcgc ggttcaaacc catcatcggc ggctaccgtt
     1801 catcgtcggg ccggacgtta cgcaagggaa ggcggagcgg caacaggaaa agcggtcgcg
//
LOCUS       DASYNS010000424         4831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1979817, whole genome shotgun sequence.
ACCESSION   DASYNS010000424 DASYNS010000000
VERSION     DASYNS010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1979817"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VGP50_13200"
     CDS             complement(<1..1013)
                     /locus_tag="VGP50_13200"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV8028384.1"
                     /translation="MDFDLTEEQRLLDETVRRLIKDEYDFEKRKTYRASPQGFSKELW
                     SRYAEIGLLGLPFPESEGGFGGTAVETMLVMENFGRGLVLEPYLATVVLGGGIVAAAG
                     NAQQKKEILPAIAEGKLMLAFAHGERQARYALADVETRAVKDGKGYVLSGAKAVVLHG
                     DGADRLVVSARTAGGARERKGITLFLVDAKAKGVERRSYPTMDGLRAAEIKLDKTPVG
                     ADAVLGTVDEGYPVLEHAVDRALAALVAEAVGITETLNTLTLDYLKTRKQFGVPIGSF
                     QALQHRMAEMVVEYEQSKSMSLLAALSADLADAKERRRVISGAKVQIGQSGRFIGQSS
                     IQL"
     gene            complement(1148..2347)
                     /locus_tag="VGP50_13205"
     CDS             complement(1148..2347)
                     /locus_tag="VGP50_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8028385.1"
                     /translation="MELAYTPEEQAFREEVRRFVQENLPPRTKDKVLNGKHLSRDDML
                     GWHRILHKKGWVAPAWPAEYGGTGWSPVQRHIFDEETAEAGAPPVIPFSITMCGPVVI
                     RFGDDWQKSYFLPRMLSAEHVWCQGFSEPGAGSDLASLRTKAVREGDHYIVNGQKTWT
                     TLAHMADWIFCLVRTDPNARKQQEGISFLLMDMKTPGITVRPIVTMDGGREINEVFLE
                     NVKVPAKNLVGEENKGWTYAKFLLGNERTGIARIGASKRELKRLKDIVSSEREDGRSL
                     IESPRFRDKLAMLEVELTALEITNLRMIAAMRGGKGPGAEASILKLKGSEIQQRISEL
                     AMEAIGPYALPFQREALEEGWNEEPIGPDYAAPLAPAYFNNRKVSIYGGSNEIQKNVV
                     AKMMLGL"
     gene            complement(2377..3678)
                     /locus_tag="VGP50_13210"
     CDS             complement(2377..3678)
                     /locus_tag="VGP50_13210"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV8028386.1"
                     /translation="MIHAFFAERASMVVPDVPADTATRLVRSAAVIGAGTMGGGIAMC
                     FANAGIPVTVIETSSEALERGLGIVRGNYAGSVARGRLEQSAMEKRLGLIKGALDLAA
                     IGDADVVIEAVFEEMAIKKEIFARLDALAKPGAVLATNTSSLDIDVIAAQTKRPQDVI
                     GMHFFSPANVMRLLEVVRGKATAKDVVATAMRLGKTFGKVPVLVGVCDGFVGNRMIFQ
                     YTREAEFLLEEGALPWQVDKALGDWGLAMGPFAMSDMAGNDVGWRIRKQQAATRPNDR
                     RYAHLADLICEKGRFGQKTNAGWYRYEKGSRAPLPDPEIEALIVAESKRQGRVRETIS
                     DAEIVKRTIYALVNEGAKILEEGIAQRPGDIDIIYVSGYGFPAWRGGPMFYADTMGLD
                     NVYADMKRFHERHGFFWRPAPLLEKLAREGRRFADLAPSRG"
     gene            complement(3877..4488)
                     /locus_tag="VGP50_13215"
     CDS             complement(3877..4488)
                     /locus_tag="VGP50_13215"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV8028387.1"
                     /translation="MAQVVRYERRGSVGVITIESPPVNALSRAVRQGLVDALLQGLGD
                     GQAKALVLIGAGRSFIAGADIREFAKPLEPPDLALVTSGFESSTKPTIAAISGPALGG
                     GLEVALACHYRIAAAAAQFGFPEVKLGIVPGCGGTQRLPRLVGVRPALDMITTGESLR
                     TTQAKAIGLVDEIVDGDLLQGALRFAERMVASGAPLPRLRDRD"
     gene            4684..>4831
                     /locus_tag="VGP50_13220"
     CDS             4684..>4831
                     /locus_tag="VGP50_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8028388.1"
                     /translation="MSSEGKVAVVTGAGTGIGRAVALALMKEGYGVALAGRRRDALER
                     TAETG"
BASE COUNT          756 a         1560 c         1690 g          825 t
ORIGIN      
        1 tggagctgga tcgaggattg gccgatgaag cggccggact gcccgatctg caccttggcg
       61 ccggaaatga cgcggcggcg ctccttggcg tccgcgaggt cggccgagag cgcggcgagg
      121 agcgacatcg atttcgactg ctcgtactcg acgaccattt ccgccatgcg atgctgcagc
      181 gcctggaaag agccgatggg cacgccgaac tgcttgcgtg tcttgaggta gtcgagagtg
      241 agggtgttga gcgtctccgt gatgccgacg gcctcggcga caagcgcggc gagggcgcgg
      301 tcgacggcgt gctcgagcac cggataaccc tcgtcgaccg ttcccagcac ggcatcggcg
      361 ccgacaggcg tcttgtcgag cttgatctcg gcggcgcgca gcccgtccat ggtgggatag
      421 gagcggcgct cgacgccctt ggccttggcg tccaccagga agagcgtgat gcccttgcgc
      481 tcgcgcgccc cgcccgcggt ccgcgccgag acgacgagcc ggtcggcgcc gtcgccatgg
      541 agcaccacgg ccttggcgcc gctcaacaca tagcccttgc cgtccttgac cgcgcgcgtc
      601 tcgacatcgg ccagcgcata acgcgcctgg cgctcgccat gggcgaaggc gagcatcagc
      661 ttgccctcgg cgatcgcggg aagaatctct ttcttctgct gagcattacc ggcagcggcg
      721 acgatgccgc cgcccaggac gacggtcgcg agatagggct cgagcacgag gccgcggccg
      781 aaattctcca tgacgagcat ggtctcgacg gcggtgccgc cgaaaccgcc ttcgctttcg
      841 ggaaaaggca ggccgagaag gccgatctcg gcatagcgcg accagagctc cttgctgaag
      901 ccttggggcg acgcgcgata ggtcttgcgc ttctcgaaat catactcgtc cttgatcagg
      961 cgccgcaccg tttcgtcgag cagcctttgt tcttcggtca aatcaaaatc catcgtgctt
     1021 tcctcgatct ccctgttccg ggtgcggtgc tcttgatcaa tcgcgaattc cgttcaaatg
     1081 gctccatgcc ccgtcatccc cgctttcgcg gggatgacga agaaagggag atgaacggaa
     1141 catgcctcta gagtcccagc atcatcttgg cgacgacgtt cttctggatc tcgttcgagc
     1201 cgccatagat cgacactttg cggttgttga agtaggcggg cgcgagcggc gcagcataat
     1261 cgggaccgat cggctcctcg ttccagccct cctcgagcgc ttcgcgctgg aagggcagcg
     1321 cgtagggtcc gatggcctcc atggcgagct cgctgatgcg ctgctggatc tcagagccct
     1381 tgagcttgag gatcgaagcc tcggcgcccg gtcccttgcc gccgcgcatg gcggcgatca
     1441 tgcgcaggtt ggtgatctcg agcgcggtca gctcgacctc gagcatggcg agcttgtcgc
     1501 ggaagcgcgg gctctcgatg agggaacggc cgtcctcacg ctcgctcgag acgatgtcct
     1561 tgaggcgctt cagctcgcgc ttcgacgcgc cgatgcgggc gatgccggtg cgctcgttgc
     1621 cgagcaggaa tttggcatag gtccagccct tgttctcctc gcccaccagg ttcttcgccg
     1681 gcaccttgac gttctcgagg aacacctcgt tgatctcgcg cccgccatcc atggtgacga
     1741 tggggcggac ggtgatgccg ggggtcttca tgtccatgag caggaaggag atgccttcct
     1801 gctgcttgcg cgcattggga tcggtgcgca ccaggcagaa gatccagtcc gccatgtggg
     1861 cgagcgtcgt ccaggtcttc tgtccgttga cgatgtagtg gtcgccctcg cgcacggcct
     1921 tggtgcgcag cgaggcgagg tcggagccgg cgccgggctc ggagaaaccc tggcaccaga
     1981 catgctcggc cgagagcatg cgcggcagga aataggattt ctgccagtcg tcgccgaagc
     2041 ggatgaccac cgggccgcac atcgtgatgc tgaaaggaat gaccggcggc gcaccggcct
     2101 ccgcggtctc ctcgtcgaag atgtggcgct gcaccgggct ccagccggtg ccgccatatt
     2161 cggcgggcca ggcgggcgcg acccagccct tcttgtggag gatgcggtgc cagccgagca
     2221 tatcgtcgcg cgagagatgc ttgccgttca ggaccttgtc cttggtccgg gggggcaggt
     2281 tctcctggac gaagcggcgg acctcctcgc ggaacgcctg ctcttccggg gtatatgcca
     2341 gttccatgtc cgggtttcct tctgagatgg cgtctctcaa ccgcgcgacg gcgccagatc
     2401 ggcgaaacgg cggccttcac gggcgagctt ctcaagcagc ggtgcgggcc gccagaagaa
     2461 accgtgccgt tcgtgaaagc gtttcatatc ggcataaaca ttgtccaggc ccatcgtgtc
     2521 cgcgtagaac atcgggccgc cgcgccaggc cgggaaaccg tagccgctga catagatgat
     2581 gtcgatgtcg ccgggccgtt gcgcgatgcc ttcctcgagg atcttggcgc cttcgttgac
     2641 cagcgcgtag atggtgcgct tgacgatctc ggcgtcgctg atcgtctcgc ggacacggcc
     2701 ctggcgcttc gattccgcga cgatcaacgc ctcgatttcg gggtcgggca gcggcgcgcg
     2761 gctgcccttc tcgtagcggt accagccggc gttggtcttc tggccgaagc ggcccttctc
     2821 gcagatgagg tcggcgagat gggcatagcg gcggtcgttg ggccgggtcg ccgcctgctg
     2881 cttgcggatg cgccagccga cgtcgttgcc ggccatgtcg ctcatcgcga agggtcccat
     2941 ggcgaggccc caatccccaa gcgccttgtc cacctgccag ggaagcgcgc cctcctcgag
     3001 aaggaactcg gcctcgcggg tgtattggaa gatcatgcga ttgccgacaa agccgtcgca
     3061 tacgccgacg agaaccggga ccttgccgaa cgtcttgccg aggcgcatgg cggtcgcgac
     3121 gacgtccttg gccgtggcct tgccgcggac cacctcgagg agccgcatga cattggccgg
     3181 gctgaagaaa tgcatgccga tgacgtcctg cggccgcttc gtctgggcgg cgatgacgtc
     3241 gatgtcgagg ctcgaggtgt tggtggcgag taccgcgccg ggcttcgcca gcgcgtcgag
     3301 cctggcgaag atctccttct tgatcgccat ttcctcgaac accgcctcga tgaccacatc
     3361 ggcgtcgccg atagcggcga gatcgagcgc gcccttgatg aggccgagac gcttctccat
     3421 ggccgattgc tcgagacggc cgcgggcgac gctgccggcg taattgccgc ggacgatgcc
     3481 gaggccgcgc tcgagcgcct cgctcgaggt ctcgatgacg gtgacgggaa tgccggcatt
     3541 ggcgaagcac atggcgatgc cgccgcccat ggtgccggcg ccgatcacgg ccgccgaccg
     3601 gaccagccgg gtcgccgtgt ccgccgggac gtcgggaacg accatcgacg cgcgctcggc
     3661 gaagaaggcg tgaatcaagg cgcgcgattg ggccccgccg accagctccg cgaaaatcgc
     3721 gcgctcgcgt ttgaggccat cgccgaaggg gagcgtgacc gcggcttcga ccgcatcgac
     3781 ggcgcggagc ggcgattcct ggccgcgcgc gcgacgcgtg acggcggcac ggtattcggg
     3841 gaagagcccg ggctcggccc gtgctgcggc aagcttctag tcgcggtcgc gcaggcgggg
     3901 caggggagcg ccggacgcga ccatccgctc ggcgaaacgc agcgcgccct ggagcagatc
     3961 gccatcgacg atctcgtcga cgaggccgat cgccttggcc tgggttgtgc gcagggactc
     4021 gcctgtcgtg atcatgtcga gcgccggtct gacgccgacg aggcggggca ggcgctgtgt
     4081 cccgccgcag ccggggacga tgccgagttt gacctcgggg aagccgaact gcgcggcggc
     4141 ggccgcgatc cggtaatggc aggcgagcgc cacttcgagc ccgccgccca gcgccgggcc
     4201 agagatcgcc gcgatggtgg gcttggtgct gctctcgaag cccgaggtca ccagagcgag
     4261 gtcgggcggc tccagcggct tcgcaaattc gcggatgtcg gcgccggcga tgaagctccg
     4321 cccggcgccg atgagaacga gcgccttcgc ctggccgtcg cccagaccct gcaggagtgc
     4381 atcgacgagc ccctgccgca ccgcgcggct cagcgcgttg accggcgggc tctcgatggt
     4441 gatgacgccc acgctgcccc ggcgctcata gcggacgacc tgcgccatct ttcctccgtt
     4501 gttcttgccc gtcccccgca cgcgacgggc gacaatagcc gagcggaccg gagaggcaag
     4561 cgccgccgcg ggttgctgcg ttagagtgct gcggccggcg cacagtctcg gcgagcgctg
     4621 gcgcgcgtgc gggggcacgt ggcagcgggc tagaatgggg atcgacgaca ccgggaggac
     4681 ggtatgagct ccgaaggcaa ggttgcggtg gtcacgggcg cgggcaccgg catcggcagg
     4741 gcggtggcgc tcgccctgat gaaggagggc tatggggtgg cgctcgccgg gcgtcggcgc
     4801 gacgcgctcg agcgcacggc ggagacggga a
//
LOCUS       DASYNS010000425         4316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3852798, whole genome shotgun sequence.
ACCESSION   DASYNS010000425 DASYNS010000000
VERSION     DASYNS010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3852798"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VGP50_13225"
     CDS             <1..385
                     /locus_tag="VGP50_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEV8028389.1"
                     /translation="QGATLIRMARDLCPHDAIDDGPYAKVVEGLDAEAAKDEKTAALL
                     GEGVKKLDASAGGNYAGEPEDKRLAALGAAAGSPFFQKVQSAMIDGFYNNQEVWAKLG
                     YEGSSAEKGGYIHRGFDDIAWLPKS"
     gene            397..1965
                     /locus_tag="VGP50_13230"
     CDS             397..1965
                     /locus_tag="VGP50_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEV8028390.1"
                     /translation="MARYDLKDDSVVVVIGSGAGGGTLANELCRKGIKVVLLEAGKRT
                     ENSDFINDEWASFNQIAWLDPRTTSGSWRVAKDFPSLPTWTCKTVGGTTTHWAGASLR
                     FQEHEFRTKTVYGQIAGANLLDWPLTLAELEPYYAKAEDKMGVTRTNNIPGLPGNNNF
                     KIMEYGATKVGYKTVHTGRMAINSVDRDDRGSCQQIGFCFQGCKSGAKWSTLYTEIPK
                     AEATGRLELRPESHALKILHDDSGKVTSVVYVDASGARQEQKARVVCVACNSIESPRL
                     LLNSESSKFPQGLANSSGVVGRNYMRHTTGSVYSIFPKPVHMYRGTTMAGIVQDEARL
                     DTKRGFVGGYELETLSLGLPFMAAFLNPGAWGREFSDALGGYANMAGMWIVGEDMPQA
                     TNRVTLNADVKDRFGMPVPNVHYDDHENDVKMREHGFRRGEEIHKAAGATKVFHTPPY
                     PSTHNLGTNRMAKNPRDGVVNKWGQSHDIKNLFISDGSQFTTGGGENPTLTIVTLAIR
                     QSDYIAHRMAKNEI"
     gene            complement(2192..2267)
                     /locus_tag="VGP50_13235"
     tRNA            complement(2192..2267)
                     /locus_tag="VGP50_13235"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2232..2234),aa:Phe,seq:gaa)
     gene            complement(2339..2530)
                     /gene="yacG"
                     /locus_tag="VGP50_13240"
     CDS             complement(2339..2530)
                     /gene="yacG"
                     /locus_tag="VGP50_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEV8028391.1"
                     /translation="MSPGSARRACPTCGKPTDPKHRPFCSARCQLIDLGRWLKGDYRI
                     PSDEPPEDGPREDDEEAEP"
     gene            2650..3402
                     /locus_tag="VGP50_13245"
     CDS             2650..3402
                     /locus_tag="VGP50_13245"
                     /inference="COORDINATES: protein motif:HMM:NF025110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyltransferase-related protein"
                     /protein_id="HEV8028392.1"
                     /translation="MTDIPPPERRYDKRLAIVVPYRDRAEHLQKFLPHLKTYFARDKL
                     DRRIAVSIHIVEPSGGGPFNRGMVKNSGYALVRDSSDYVCFHDVDYLPIWADYGWSAQ
                     PARLIWHGLALQEDRQRFFGGVVLFDNAAFARVNGYPNAYWGWGPEDLELGFRCDLSG
                     LGFERRDGTYMALPHPNAGFTAPGVHSEEARRTHVVWAERTPRLERLMAEDGLASLKF
                     TPLRQTRVTLEGQDQPPIFHHLIDIGAPPDRR"
     gene            complement(3399..>4316)
                     /locus_tag="VGP50_13250"
     CDS             complement(3399..>4316)
                     /locus_tag="VGP50_13250"
                     /inference="COORDINATES: protein motif:HMM:NF021653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEV8028393.1"
                     /translation="AAAGAAPAEVVAEAEALRARWRAIVARSAARRAPALLEDAEPAV
                     AMLLAELAAAPLDAIVIDDRAAFAEARQWLARDRPALAGQLALHRETSPLFEHYGIAE
                     DVAAALAPRIALPDGVAVTIEHSAAATLIDVDSGSTAAAGLAANLRAAREVARQIRLR
                     NLAGPIVVDFIGMRDKGQRERVRVLLAELLALDGDSDVLGWTRLGHLELVRKRRHAPL
                     AELLFERVPGGGLRKTAATVALEALRAVARDAAASPGRAPALRVAPEVAAALDGAARA
                     GRQALEARLGRPLAVTAEPGRLRDAFDIG"
BASE COUNT          745 a         1497 c         1425 g          649 t
ORIGIN      
        1 gcaaggcgcg acgctcattc gcatggcgcg cgacctctgc cctcatgacg ccatcgatga
       61 cggtccctac gccaaggtcg tcgagggcct cgatgcggag gcggccaagg acgagaagac
      121 cgctgcgctg ctcggcgaag gcgtgaagaa gctcgatgcc tcggccggcg gaaactatgc
      181 cggcgagccg gaagacaagc gtcttgcggc gcttggcgcc gctgccggca gtccgttctt
      241 tcagaaggtg cagagcgcca tgatcgacgg cttctacaac aaccaggagg tctgggcgaa
      301 gctcggctac gaagggtcct ccgccgaaaa aggcggctac atccatcgtg gcttcgacga
      361 catcgcctgg ctgccgaaga gctgagggag gaagccatgg ccagatacga cctcaaggat
      421 gattcggttg tcgtggtgat cggctcgggc gcgggcggcg gcacgctcgc caacgagctt
      481 tgccggaaag ggatcaaagt ggtcttgctc gaggcgggca agcgcaccga gaacagcgac
      541 ttcatcaacg acgagtgggc gtcgttcaac cagatcgcct ggctcgaccc gcgcacgacc
      601 tcgggcagct ggcgggtcgc caaggacttc ccgagcctcc cgacctggac ctgcaagacg
      661 gtgggcggca cgaccacgca ttgggccggc gcctcgctgc gcttccagga gcacgagttc
      721 cgcaccaaga ccgtctatgg ccagattgcc ggagcgaacc tgctcgattg gccgctcacc
      781 ctcgccgagc tcgagcctta ttacgccaag gccgaggaca agatgggggt gacgcgcacc
      841 aacaacatcc cgggacttcc cgggaacaac aacttcaaga tcatggaata cggcgcgacg
      901 aaggtcggct acaagaccgt gcacaccggc cgcatggcca tcaactcggt cgatcgcgac
      961 gaccgcggct cgtgccagca gatcggcttc tgcttccagg gctgcaagtc cggcgccaaa
     1021 tggtcgactc tttacacaga gataccgaaa gcggaggcga ccggccggct cgagctgcgt
     1081 ccggaaagcc atgcgctgaa gatcctgcat gacgacagcg gaaaagtgac cagtgtcgtc
     1141 tatgtcgacg ccagcggcgc ccgccaggag cagaaggcgc gcgtcgtctg cgtcgcctgc
     1201 aattcgatcg agagtccgcg tctcctgctg aactcggaat cgagcaagtt cccgcagggg
     1261 ctcgcgaact cctcgggcgt ggtgggccgg aactacatgc ggcacaccac ggggtcggtc
     1321 tactcgatct tcccgaagcc cgtgcacatg tatcgcggca cgacgatggc cgggatcgtc
     1381 caggacgagg cccggctcga caccaagcgc ggcttcgtcg gcggctatga gctcgagacg
     1441 ctgtcgctcg gattgccgtt catggccgcg ttcctcaatc ccggcgcctg ggggcgggaa
     1501 ttttccgatg cgctcggtgg ctacgccaac atggcgggca tgtggatcgt cggcgaggac
     1561 atgccgcaag cgacgaaccg cgtgacgctc aacgccgacg tcaaggaccg cttcggcatg
     1621 ccggtcccga acgtgcatta cgacgaccat gagaacgacg tgaagatgcg cgagcacggc
     1681 ttccggcgcg gcgaggagat ccacaaggcg gcgggcgcaa cgaaggtgtt tcacacgccg
     1741 ccctatccct cgacacacaa tctcggcacc aaccgcatgg ccaagaaccc gcgtgacggc
     1801 gtcgtcaata aatggggcca gagccacgac atcaagaacc tgttcatctc ggacggcagc
     1861 cagttcacga ccggaggtgg cgagaacccg acgctcacca tcgtgacctt ggccataagg
     1921 cagtcggact acatcgccca ccgcatggcg aagaacgaga tctgacgctg cgcccgacgc
     1981 ggcggcccgt cgtcggcgct gccgcgtccc ttcgctcgct cgcaatcagc gcgcgacgct
     2041 gcggccgcgg tgttttgtct tcatgctaac aaacggtcgc ttcacccgtc acccgcaccg
     2101 cccccacagc gaggcttgtg ccgcgcgctt accgtgagct tccggcgcgc aagccgccca
     2161 tggcgaccca ctcgtaagtg cctgaaaaga atggtgccca ggggcggaat tgaaccaccg
     2221 acaccgtgat tttcagtcac gtgctctacc aactgagcta cctgggcgcc gtgccggacg
     2281 gggcgcgggc ttatgccaga aaagcccacc cctgtccaga agcggccacc ccgcggtctc
     2341 acggctcggc ttcctcgtca tcctcgcggg gcccgtcttc cggcggctcg tcgctgggaa
     2401 tgcggtagtc gcccttgagc caacggccca gatcgatcag ctggcagcgc gccgagcaga
     2461 agggccgatg tttcgggtcg gtgggcttgc cgcaggtcgg acaggcccgt ctggcggagc
     2521 ctggactcat gtcactcatg ccccataaat agggctctcc gacggggctg tcgaagggcg
     2581 ctctgccgct ctcgcgcttg ttaccgccat gcgacgcgtc cgcagcctag agttggcagc
     2641 agcatgagcg tgacggacat tcccccaccc gagcggcgct atgacaagcg cctcgccatc
     2701 gtcgtcccct atcgggaccg cgccgagcat ctccagaaat tcctccccca cctcaaaacc
     2761 tatttcgcgc gcgacaagct cgatcgccgg atcgccgtct cgatccacat cgtcgagccg
     2821 tccggcggcg ggccgttcaa ccgcggcatg gtgaagaaca gcggctacgc gctcgtcagg
     2881 gacagctccg actatgtctg ctttcacgac gtcgactacc tgccgatctg ggcggattac
     2941 ggctggtcgg cgcagcccgc ccggctgatc tggcacggcc tcgccctcca ggaggatcgg
     3001 cagcggttct tcggcggcgt cgtcctcttc gacaatgcgg cgttcgcgcg cgtcaacggc
     3061 tatcccaatg cctattgggg ctggggaccc gaggatctgg aactgggctt ccgctgcgac
     3121 ctctccggcc ttggcttcga gcgccgcgac ggcacctaca tggctttgcc gcaccccaat
     3181 gccggtttca cggcgccggg cgtccattcg gaagaggcgc gccgcaccca tgtggtctgg
     3241 gcggagcgga cgccgcggct cgagcgcctg atggcggaag acggtctcgc gagcctcaaa
     3301 ttcacgccgc ttcgccagac gcgcgtgacc ctcgagggcc aggaccagcc gccgatcttc
     3361 caccatctca tcgacatcgg cgcgccgccc gaccggcgct agccgatatc gaaggcgtcg
     3421 cggagacggc ccggctcggc ggtgaccgcg aggggccggc cgaggcgcgc ctcgagcgcc
     3481 tggcggccgg cgcgcgccgc gccatcgagc gccgcggcca cctccggagc cacccgcaat
     3541 gctggcgcgc ggccgggcga tgccgcggcg tcgcgggcca cggcgcggag cgcttcgagg
     3601 gcgacggtcg cggccgtctt ccgcaagcct ccgcccggca cgcgctcgaa caacagctcg
     3661 gcgagcggcg cgtggcggcg cttgcgcacc agctcgagat ggccgaggcg cgtccacccc
     3721 agcacgtcgc tatcgccgtc cagagccagg agctcggcga ggagcacgcg cacgcgctcg
     3781 cgctgaccct tgtcgcgcat gccgatgaaa tcgacgacga tcggtccggc aaggttgcgc
     3841 agccggatct gccgcgccac ttcgcgcgcc gcccgcagat tggccgcaag cccggccgcg
     3901 gcagtgctgc cgctgtcgac atcgatgagc gtagcggcgg cgctgtgctc gatcgtcacc
     3961 gcgacgccgt ccggcaacgc gatccgcggc gcgagcgccg cggcgacgtc ctccgcgatc
     4021 ccgtaatgct cgaagagcgg cgaggtttcg cggtgcagag ccaattggcc cgccagcgcc
     4081 ggccgatccc gcgcgagcca ttgccgcgct tcggcgaagg ccgcgcggtc gtcgatgacg
     4141 atcgcgtcga gcggtgcggc cgcgagctcg gcgagcagca tggcgacggc gggctcggcg
     4201 tcctcgagca gcgccggcgc gcggcgtgcg gcactccgtg cgacgatcgc gcgccagcgc
     4261 gcgcgcagcg cttccgcttc ggcgaccacc tccgccggag cggcgccggc ggcggc
//
LOCUS       DASYNS010000426         6322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4166227, whole genome shotgun sequence.
ACCESSION   DASYNS010000426 DASYNS010000000
VERSION     DASYNS010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4166227"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VGP50_13255"
     CDS             complement(<1..176)
                     /locus_tag="VGP50_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEV8028394.1"
                     /translation="MFAKILIANRGEIACRVIRTAQRMGIRTVAVYSEADAGALHVAM
                     ADEAYPIGAAPARE"
     gene            complement(180..968)
                     /locus_tag="VGP50_13260"
     CDS             complement(180..968)
                     /locus_tag="VGP50_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV8028395.1"
                     /translation="MSAPAILVSSDARGVATLTLNRPAVHNAFDDALIAELTSSLRRA
                     AAETAVRVVVLTGSGQSFSAGGDLNWMRRMAQFGDAENRADAMALATLMRTLDELGKP
                     TVARVNGAAFAGGLGLVSCCDIAIAAEEAVFSLSEARLGLVPATIAPYVVAAIGPRAA
                     RRYCLTAERFSASEALRLGLVHEVVPRASLDATVEKIVAALLEAAPGAQARAKRLIAE
                     VQGRPLGDATIALTAQVIADARASAEGREGLASFFEKRKPAWRA"
     gene            complement(973..2580)
                     /locus_tag="VGP50_13265"
     CDS             complement(973..2580)
                     /locus_tag="VGP50_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV8028396.1"
                     /translation="MPVLTTALDSRSESFRSNAKAMQALVADLATQVADIRKGGGEAA
                     RERHLDRGKLLPRDRLRALLDPGAPFLEFSQLAAHGMYGSEVPAAGIITGIGPVIGRE
                     CVVVVNDATVKGGTYYPVTVKKHLRAQEIARQNNLPCLYLVDSGGANLPNQDEVFPDR
                     EHFGRIFYNQATLSAAGIPQIAIVMGSCTAGGAYVPAMSDECIIVRNQGTIFLGGPPL
                     VKAAIGEVVTAEELGGAEVHSRISGVTDHYAQDDAHALGIARRIVANLHRVKRPALDI
                     AEPVEPLYDATELHGVVPTDPRKPYDAREVIARVVDGSVLDEFKPLYGQTLVTGFARI
                     WGYPVGIVANNGILFSESALKGAHFIEICAQRGIPLVFLQNITGFMVGRKYEAGGIAK
                     DGAKMVTAVACANVPKFTMVVGGSFGAGNYAMCGRAYGPRFLWMWPNARISVMGGEQA
                     ASVLAQVRRDNMEARGEAWSGDDEETFKEPIRAQYEKQGHPYYATARLWDDGVIDPAD
                     TRRVLALGLSASLNAPIEKTQFGVFRM"
     gene            complement(2727..3119)
                     /locus_tag="VGP50_13270"
     CDS             complement(2727..3119)
                     /locus_tag="VGP50_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HEV8028397.1"
                     /translation="MPELGINPEKVCAFIEVAREVSALEPSTAGDTMTEGDDGPLAEM
                     IERPRGMDPRRREMVAFVAGLDGGEQVNLLALILLGRGDFAIEEWEDALVAARDSIDD
                     RSADFMVGDSALPGYLLEALEAFDESCA"
     gene            complement(3197..4333)
                     /locus_tag="VGP50_13275"
     CDS             complement(3197..4333)
                     /locus_tag="VGP50_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8028398.1"
                     /translation="MMLSQEQTAIRDMARRFAADRLKPFAAEWDRAARFPAEAVAEMG
                     QLGLLGMLVPEEYDGAAADHVSYALAVEEIAAGDGACSTIMSVHNSVGCVPVLRFGTA
                     QQKERFLKPMARGEMLAAFCLTEPAAGSDASAIKTRARRAGNRYLLSGTKQFITSGAH
                     AKVAIVFAVTAPDSGKKGISAFIVPTDLPGWRVARVEHKLGQRASDTAQIVFDEMALT
                     PDLLLGEEGEGYRIALANLEGGRIGVGAQSVGMARAAFEAATSYARERRSFGVPIADH
                     QAVAFRLADMATRIEAARQLVLHAAALRDAGVPCLKEAAMAKLYASEMAERVCSDAIQ
                     IHGGYGYLQDFPVERIYRDVRVCQIYEGTSDIQRLVISRQILAE"
     gene            4471..6138
                     /locus_tag="VGP50_13280"
     CDS             4471..6138
                     /locus_tag="VGP50_13280"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEV8028399.1"
                     /translation="MSDPESGAPGFTTHEVFNQPPPLADYNVFASDRALVEAVEREGG
                     SWAKDRLSAFGKRLGRAETIDWGFQANRNSPVLQTFDRFGQRRDTVEFHPAYHQLLAL
                     AVDAGVHTAPWAEPRAGAHVARGATVHMQCQIEASVQCPLTMTYGVVPALRHQADIAA
                     EWLPRLYSRRYDPRFLPAGAKTGALMGMGMTEKQGGSDLRSNTTRAEPEGAGGPGAAY
                     RLVGHKWFLSAPMCDAFLVLAQAPKGPSCFFLPRWTPDGQVNALRLQRLKDKLGNRAN
                     ASSEVEFQNARAWLVGEEGRGIPGIIEMATYTRLDCALGTAGLMRQAVAQAIHHARHR
                     TVFQRHLVDQPLMTSVLADLALESEAATALALRLARAFDRQDDPSETAFRRLMTPAAK
                     YWICKRGITLAGEAMEVLGGNGYVEDSLMPRIYREMPVNSIWEGSGNVMCLDVLRALQ
                     RTPEAGEALRAELAPARGTEARLDRFLERLDSTLAARPGEAQARRLAEMLVLAVQGAL
                     LVRFAPPAVADAFCASRLEGDRSGAFGTLPAGIDAAAIVERAAPAIA"
BASE COUNT          998 a         2201 c         2143 g          980 t
ORIGIN      
        1 ctctcgcgcg cgggcgcagc gccgatcgga taggcctcat cggccatggc gacatggagc
       61 gcgccggcat cggcctcgga atagacggcc accgtgcgga tgcccatgcg ctgcgcggta
      121 cggatgaccc ggcaggcgat ctcgccacga ttggcgatga ggatcttcgc aaacatcgct
      181 caggcccgcc aggccggctt gcgcttctcg aagaaggaag cgagcccttc gcgtccctcg
      241 gccgacgcgc gtgcgtcggc gatgacctgt gcggtgagcg cgatggtcgc atcgccgagc
      301 ggacggccct gcacttcggc aatgaggcgc ttggcgcgcg cctgcgcgcc cggcgccgcc
      361 tcgagcagcg ccgcgacgat cttctcgacc gtcgcatcga gcgatgcgcg cggcacgacc
      421 tcgtgcacga ggccgagacg cagcgcttcg ctcgccgaga agcgctccgc ggtgaggcaa
      481 tagcgtcgcg ccgcccgcgg cccgatcgcc gcgacgacat aaggtgcgat ggtcgccggc
      541 accagcccga gccgcgcctc cgacagcgag aacacggcct cctcggcggc gatagcgata
      601 tcgcaacagg agacgagacc gagaccgccg gcaaaggccg cgccgttgac gcgggccacg
      661 gtcggcttcc cgagctcgtc gagggtgcgc atcaatgtcg cgagcgccat ggcgtcggcg
      721 cggttctcag catcgccgaa ttgcgccatg cgccgcatcc agttgaggtc gccgccggcg
      781 gagaagctct gcccgctgcc ggtgagcacg acgacgcgca ccgccgtctc cgccgcggcg
      841 cgccgcagcg atgacgtgag ctcggcgatg agcgcatcgt cgaaggcgtt gtgaaccgcg
      901 ggacggttga gggtcagcgt ggcgacgccg cgggcgtcgc tgctcacgag gatggcgggc
      961 gcgctcattg cgtcacatcc ggaagacgcc gaactgcgtc ttctcgatcg gcgcgttgag
     1021 gctcgccgag agaccgagcg ccagcacgcg gcgcgtgtcg gcggggtcga tgacgccgtc
     1081 gtcccagagc cgggcggtcg cataataggg atggccctgc ttctcatatt gcgcgcggat
     1141 cggctccttg aaggtctcct cgtcgtcgcc gctccatgcc tcgccgcgcg cctccatgtt
     1201 gtcgcgccgc acttgcgcca gcaccgacgc cgcctgctcg ccgcccatga cggagatccg
     1261 cgcattgggc cacatccaaa ggaagcgggg cccgtacgcg cggccgcaca tggcgtagtt
     1321 gccggcgccg aaactgccgc cgaccaccat ggtgaatttc ggcacgttgg cgcaggccac
     1381 cgcggtcacc atcttcgcgc cgtccttggc gatgccgccc gcctcgtatt ttcggcccac
     1441 catgaatccg gtgatattct gcaaaaacac gagcggaatg ccgcgttgcg cgcagatttc
     1501 gatgaaatgc gcgcctttca gcgcgctttc cgagaaaaga atgccgttat tggcgacgat
     1561 tccgacggga tagccccaaa ttcgcgcaaa acccgtgacc agcgtctggc cgtagagcgg
     1621 cttgaactcg tcgagcacgc tgccgtcgac gacgcgggca atgacctcgc gtgcgtcata
     1681 gggcttgcgc ggatccgtcg gcaccacgcc atggagctcg gtcgcgtcat agagcggctc
     1741 cacgggctcg gcgatgtcga gcgccggccg cttcacgcga tggagattgg cgacgatgcg
     1801 ccgcgcgatg ccgagcgcat gcgcgtcatc ctgcgcgtaa tggtcggtga cgccggagat
     1861 ccggctgtgc acctcggcgc cgccgagctc ttcggcggtc accacctcgc cgatcgccgc
     1921 cttcacgagt ggcgggccgc cgagaaagat cgtgccctgg ttgcgcacga tgatgcattc
     1981 gtccgacatc gccggcacat aagcgccgcc cgccgtgcac gagcccatga cgatcgcgat
     2041 ctgcggaatg cccgccgccg agagcgtggc ctggttatag aagatgcggc cgaaatgctc
     2101 gcggtccgga aacacctcgt cctggttcgg caggttggcg ccgccggagt cgacgagata
     2161 gaggcacggc agattgttct gccgcgcgat ctcctgggcg cgcagatgct tcttgacggt
     2221 gacgggataa tacgtcccgc cctttaccgt cgcgtcgttg acgacgacga cgcattcccg
     2281 ccctatgacc gggccgatgc cggtgatgat gccggccgcc ggcacctcgc tgccatacat
     2341 gccatgggcc gcgagctgcg agaattcgag gaagggcgca ccgggatcga ggagcgcccg
     2401 caagcggtcg cgcggcagga gcttgccgcg gtcgagatgc cgctcccgcg ccgcctcgcc
     2461 gccgcctttg cggatgtcgg cgacctgtgt cgcgagatcg gcgaccaggg cctgcatggc
     2521 cttggcattg ctgcggaacg attcggagcg cgaatcgagc gcggtggtga gcaccggcat
     2581 gggaacggac gtccttgagg ccgcggcgcg cgcgggttgc ccgccaccat gcaaaagcgc
     2641 atcgcagggg gcaaggggaa aaaggcggat cccgccgggc cccctcaccc tccctctccc
     2701 acaaggggag agggaattcc ggcgatttag gcgcagctct cgtcgaaggc ctcgagcgcc
     2761 tcgaggagat agccgggaag cgccgagtcg ccgaccatga agtcggcgct gcgatcatcg
     2821 atgctgtcgc gcgccgcgac aagggcgtct tcccactcct cgatggcgaa gtcgccgcgg
     2881 cccagcagga tgagggccag aagattgacc tgctccccgc catcgagacc ggcgacgaag
     2941 gcgaccattt cgcgccgccg cgggtccatc ccgcgcggcc gctcgatcat ctcggcgagc
     3001 gggccgtcgt ctccctcggt catcgtgtca ccggcggttg agggctcgag cgccgacacc
     3061 tcgcgcgcga cttcgatgaa ggcgcagacc ttctctgggt tgataccgag ctcgggcatc
     3121 gcttgcccca ttgctattgg cgatcccttg ctaacggcgc gcgccgccgc tcgatccagg
     3181 cgcggcgcgg cgcaagctac tcggccagga tctgccggct gatcaccagc cgctggatgt
     3241 cgctggtgcc ttcatagatc tggcacacgc gcacatcgcg atagatccgc tccacgggaa
     3301 aatcctggag atagccatag ccgccatgga tctggatggc gtcggagcag acgcgctccg
     3361 ccatctcgga agcatagagc ttggccatgg ccgcttcctt gaggcagggc acgccggcat
     3421 cgcgcaacgc cgccgcgtgc agcacgagct ggcgcgcggc ctcgatccgc gtcgccatgt
     3481 cggcgaggcg gaacgccacc gcctgatggt cggcgatcgg cacgccgaag ctccgccgct
     3541 cgcgcgcgta tgacgtggcg gcctcgaaag ccgcccgcgc catgccgacc gactgcgcgc
     3601 cgacgccgat gcgcccgcct tcgagattgg cgagcgcaat gcgatagcct tcgccttctt
     3661 cgccgagcag gagatcgggc gtgagcgcca tctcatcgaa gacgatttgc gccgtgtcgg
     3721 aggcgcgttg gccgagcttg tgctcgaccc tggcgacgcg ccagccgggc agatcggtcg
     3781 gcacgatgaa ggcgctgata cccttcttgc cggaatcggg cgcggtcacc gcaaagacga
     3841 tcgccacttt cgcgtgcgcg cccgaggtga tgaactgctt ggtgccgctg aggagatagc
     3901 gattgccggc gcgacgcgcg cgcgtcttga tggccgaagc gtccgagccc gccgcgggct
     3961 cggtaaggca gaaggcggcc agcatctcgc cgcgcgccat cggcttgagg aagcgctcct
     4021 tctgctgggc cgtgccgaag cgcaacacgg ggacgcagcc caccgaatta tgcacgctca
     4081 tgatggtcga gcaggcgccg tcgccggcgg caatctcctc gacggcgagt gcatacgaga
     4141 cgtggtcggc tgcggcgccg tcatattctt cgggcacgag catgccgagg agaccgagct
     4201 ggcccatctc cgccacggcc tcggcgggaa aacgcgcggc gcggtcccat tcggcggcaa
     4261 agggcttgag ccggtcggcc gcgaagcgcc gcgccatgtc gcgaatggcc gtctgctcct
     4321 gcgacagcat catgcggcga gcctaccatg ggatgggctt gccgtcctgc gtgcagaagc
     4381 ggccgcggtc cgccggttct cgacagacac ccgcgttcct gcggccgtcc tggccatggt
     4441 gtaagatcgc gcgcacgagg aggatccgcc atgagcgacc ccgagagcgg cgcgcccggc
     4501 ttcacgaccc atgaggtctt caaccagccg ccgccgctcg ccgattacaa cgtcttcgcc
     4561 agcgaccgcg cgctggtcga ggcggtggag cgcgaaggcg ggagctgggc aaaggaccgg
     4621 ctctccgcct tcggcaagcg gctcggccgc gcggagacga tcgactgggg ctttcaggcg
     4681 aaccgcaatt cgccggtgct gcagactttc gaccgcttcg gacagcggcg cgacacggtc
     4741 gagttccacc ccgcctatca ccagctgctg gcgctcgccg tcgatgccgg cgtgcacacc
     4801 gcgccttggg cggagccgcg ggcgggcgct catgtcgcgc gcggcgccac tgtccatatg
     4861 cagtgccaga tcgaggccag cgtgcaatgt ccgctcacca tgacctatgg cgtggtcccc
     4921 gcgctccggc accaggccga catcgccgcc gaatggctgc cccgcctcta ctcgcggcgc
     4981 tacgacccgc ggtttctccc cgccggcgcg aaaaccggtg cgctcatggg catgggcatg
     5041 accgagaagc agggcggctc cgatctccgc agcaacacga cgcgcgccga gcccgaaggc
     5101 gcgggcggac ccggcgccgc ctatcgcctt gtcggccaca aatggtttct ctcggcgccg
     5161 atgtgcgacg ccttcctggt gttggcgcag gcgcccaaag gtccctcctg cttcttcctg
     5221 ccgcgctgga cgcccgatgg ccaggtcaat gcgctgcgcc tgcagcggct caaggacaag
     5281 ctcggcaacc gcgccaatgc ctcgagcgag gtcgagttcc agaacgcgcg cgcctggctc
     5341 gtcggcgagg aaggacgcgg catccccggc atcatcgaga tggcgaccta tacacggctc
     5401 gattgtgcgc tgggtaccgc cgggctgatg cgccaggccg tggcgcaggc aattcaccat
     5461 gcgcgccacc gcacggtctt ccagcgccat ctcgtcgacc agcccttgat gacgagcgtg
     5521 cttgccgatc tcgccctcga atcggaagcg gcgaccgcct tggccttgcg cctcgcccgc
     5581 gccttcgacc gccaggacga tcccagtgag accgccttcc gccgcctgat gacgccggcc
     5641 gccaaatact ggatctgcaa gcgcggcatc acgctcgccg gcgaggccat ggaagtgctg
     5701 ggcggcaacg gctatgtcga ggacagcctg atgccgcgca tctatcgcga gatgcccgtc
     5761 aactcgatct gggaaggctc gggcaacgtc atgtgcctcg atgtcctgcg cgccttgcag
     5821 cgcacgcctg aagcgggcga ggcgctgcgc gccgagctcg cgccggcacg gggcaccgag
     5881 gcgcggctcg atcgcttcct cgagcgcctc gactcgacgc tcgccgcgcg gcccggcgag
     5941 gcacaggcgc ggcgcctcgc cgagatgctg gtgctcgccg tgcaaggcgc cctcctcgtc
     6001 cgcttcgccc ctccggcggt tgccgacgcg ttttgcgcca gccgcctcga gggcgaccgc
     6061 agcggcgcct tcgggactct gccggcggga atcgacgccg ctgcgatcgt cgagcgggcc
     6121 gcgccggcga tcgcttgatt gcttcggacg caaatgaact ggcgatcgcg ctcaatcatt
     6181 ccctctcccg ataggggaga gggagatgag tttggcgttc gtggcaaatg tcggagactt
     6241 cgatggccgg acgcgtctcc gtggtgatcc cgacctcgac gaagccccgc gcatcggcgc
     6301 gccgatcatc gccgtgccta cg
//
LOCUS       DASYNS010000427         3475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5411684, whole genome shotgun sequence.
ACCESSION   DASYNS010000427 DASYNS010000000
VERSION     DASYNS010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5411684"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            374..>3475
                     /locus_tag="VGP50_13285"
     CDS             374..>3475
                     /locus_tag="VGP50_13285"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV8028400.1"
                     /translation="MDLASLFHPAVAAWFKSRFAAPTAAQAEAWPAIKDGRNVLIAAP
                     TGSGKTLAAFLAAIDSLVRQGLEGALANETQIVYVSPLKALSNDIRRNLEAPLAGIGE
                     ALRAQGLPEIEIRSWVRTGDTPQGERDRARRKPPHIVVTTPESLYILLGSEGGRKMLQ
                     TTRTVIVDEIHAVAPNKRGAHLAVSLERLDALVGRKLLRIGLSATQKPIEDVAHFLVG
                     TEGGACRIIDTGHRRARDLALEVPLSPLESVMSGEVWEQVYDRLALLVEAHRTTLIFV
                     NTRRMTERVTRALSERLGAENVAAHHGSLAKERRLDAEQRLKNGQLKALVATASLELG
                     IDIGEVDLVCQLGSPRRIAAFLQRVGRSGHAVDGTPKGRLFPLSRDELVECAALLDSV
                     NRGELDRLAIPDRPLDVLAQQIVAEAAAREWTEDDLFALIRRAWPYRDLPRADFAAVL
                     TTLAEGYASRRGRRGALVHWDAVNHKLRGRRGARLVALTSGGTIPDTADYQVLLEPEN
                     QMIGSVNEDFAVESMQGDVFQLGNSSYRILRVERGTVRVADAEGQAPTIPFWLGEAPG
                     RSDELSRSVSRLRSDFAAHLPAERALPWLIDEVGIAEAAATQLVDYLAAGRAALGVLP
                     THETLVIERFFDEVGGTQLVIHSPYGSRLNRAWGLALRKRFCRKFNFELQAAATEDNI
                     VLSLTQAHSFDLDDVPRYLHTASVRDVLVQALLDAPMFNTRWRWVAAVSLALPRFRGG
                     RKVPPQLARMDAEDLIAAIFPDQIACAENLVGEREIPDHPLIRQTIADCLNEAMDFDG
                     LMGLLRGLESGAIRVVTRDLTEPSPLAQEVLSARPYAYLDDAPLEERRTQAVMSRRWL
                     APEAASDLGRLDAEAIDRVRGEAWPEATNADELHDALVWLGFIAAAEAERASGWGDWL
                     TALLREKRAALLHVPETAAIWIAAERVTLFQAMFPAARLEPAIVAPPALAAQHWSRED
                     ALVEILRGRLEGLGPVTVPALAAPLGVAVDAIAAALAALQSEGFVMRGRFTPGVVEDE
                     WCER"
BASE COUNT          510 a         1233 c         1175 g          557 t
ORIGIN      
        1 taaagatttg tcgaggcaca cgccatgacc gagccgatcg actaccttga gattggacgc
       61 gtagcccatg agcttgctga aaggcacggg cggaacgctc actgctatgc aacgaagcaa
      121 gccgaacttg ccctggtcaa aggcgcgata gacgagcaac gtttctggga gtcggtcacc
      181 gccgcgctaa tgccacgcga gagctcatag agtcactgtg ttcggagctg gcgcgcgtcg
      241 atgtcgaccg aggctgccga ttcgggtcga cgaccgtaac cgcgctcact cggcgattcg
      301 ccggtgttca tgcggagcgc gtgctccgtt gtgcaaggat tgattcacgc ctgaatcccc
      361 ccattattcc cggatggacc tcgcttctct cttccatccg gccgtcgccg cctggttcaa
      421 aagccgcttc gcagccccga ccgcggcgca ggccgaggct tggcccgcca tcaaggacgg
      481 ccgcaacgtc ctgatcgcgg cgcccacggg ctcgggcaag acgctcgccg cctttctggc
      541 ggccatcgat tcgctggtgc ggcaggggct cgaaggcgcg ctcgcgaacg agacgcagat
      601 cgtctacgtg tcgccgctca aggcgctctc gaacgacatc cgccgcaacc tcgaggcgcc
      661 gctggccggg atcggcgagg cgctccgtgc ccagggcctg cccgaaatcg agatccgcag
      721 ctgggtccgc accggggaca ccccgcaagg cgagcgcgac cgcgcgcggc gcaagccacc
      781 gcatatcgtc gtcaccacgc ccgaatccct ctacatcctg ctcggctccg agggcgggcg
      841 taagatgctg cagacgacgc gcacggtgat cgtcgacgag atccatgcgg tggcgcccaa
      901 caaacggggc gcccatctcg cggtctcgct cgagcgtctc gatgcgctcg tcgggcggaa
      961 gctgctgcgc atcggtctct cggcgacgca aaagccgatc gaggacgtcg cgcatttcct
     1021 ggtcggcacc gaaggcggcg cgtgtcgcat catcgatacc ggccatcgcc gcgcccgcga
     1081 cctggcgctc gaagtgccgt tgtcgccgct tgaatcggtg atgtcgggcg aggtctggga
     1141 gcaggtctat gatcggctgg cacttctcgt tgaggcccat cgcacgacgc tgatcttcgt
     1201 caacacgcgg cgcatgaccg agcgtgtgac gcgcgcgctt tcggagcgtc tcggcgccga
     1261 gaatgtcgcg gcgcatcacg gcagcctcgc caaggagcgc cgcctcgacg ccgagcagcg
     1321 cttgaagaac ggccagctca aggcgctggt ggcgacggcc tcgctcgagc tcggcatcga
     1381 tatcggcgag gtcgatctcg tctgccagct cggctcgccg cgccgcatcg ccgccttcct
     1441 gcagcgcgtc ggacgctccg gccatgccgt cgacggcacg cccaagggac gtctgttccc
     1501 gctctcgcgc gacgagctcg tcgaatgcgc ggcgctcctc gacagcgtca accgcggcga
     1561 gctcgaccgt ctcgcaatcc ccgaccgtcc cctcgacgtg ctggcgcagc agatcgtggc
     1621 agaggccgcc gcgcgcgaat ggacggaaga cgatctcttc gcgctcatcc gccgcgcctg
     1681 gccgtatcgc gatctgccgc gcgcggattt cgcggcggtg ctcacgacgc tggccgaagg
     1741 ctatgccagc cgccgcggcc ggcgcggcgc gctcgtccat tgggatgcgg tgaaccacaa
     1801 gctgcgcggg cggcgcggcg cgcggctcgt ggcgctgacc tccggcggca cgatcccgga
     1861 cacggccgat taccaggtgc tgctcgagcc cgagaaccag atgatcggca gcgtcaacga
     1921 ggacttcgcc gtcgagagca tgcagggcga cgtcttccag ctcggcaaca gctcctatcg
     1981 catcctgcgc gtcgagcgcg gcacggtccg cgtcgccgat gcggagggtc aggcgccgac
     2041 gatcccgttc tggctcggcg aagcgcccgg acgcagcgac gagctctcgc gctcggtctc
     2101 gcgcttacgc tcggatttcg ccgcgcatct gccggcggag cgcgctttgc cctggctcat
     2161 cgacgaggtc gggatcgccg aggcggcggc gactcagctc gtcgattatc tcgcggcggg
     2221 acgcgcggcg ctcggcgttc tgccgacgca cgagacgctg gtgatcgagc gcttcttcga
     2281 cgaggtcggc ggcacgcagc tcgtcatcca ctcgccttac ggcagccgcc tcaaccgcgc
     2341 ctggggcctg gcgctgcgca agcgcttctg ccgcaagttc aatttcgagc tgcaggcggc
     2401 cgcgaccgag gacaacatcg tcctgtcgct gacccaggcg cacagcttcg atctcgatga
     2461 cgtgccgcgc tacctccata cggcgagcgt gcgcgacgtg ctggttcagg cgctgctcga
     2521 cgcgccgatg ttcaacacgc gctggcgctg ggtcgccgcc gtgtcgctgg cgctgccgcg
     2581 cttccgcggc ggccgcaagg tgccgccgca gctcgcgcgg atggatgccg aggacctgat
     2641 cgccgccatc ttccccgacc agatcgcctg cgccgagaat ctggtcggcg agcgcgagat
     2701 ccccgaccat ccgctgatcc gccagacgat cgccgactgc ctcaacgaag ccatggattt
     2761 cgacggcctc atgggcctct tgcgcggtct cgaatccggc gcgatccgcg tcgtcacgcg
     2821 cgatctcacc gagccctcgc cgctggcgca ggaggtgctg tcggcgcgtc cctacgccta
     2881 tctcgacgac gcacctctcg aggagcgccg cacgcaagcg gtgatgagcc ggcgctggct
     2941 cgcgcccgag gccgcgtccg atctgggccg tctcgatgcc gaggcgatcg accgtgtccg
     3001 cggcgaagcc tggcccgaag cgaccaatgc cgatgagctg catgacgcgc tggtgtggct
     3061 cggcttcatc gccgccgccg aggccgagcg cgcttccggt tggggcgatt ggctgacggc
     3121 gctcctgcgc gagaagcggg cggcgctgct gcatgtgccg gagaccgccg ccatttggat
     3181 cgccgccgag cgcgtgacgc tgtttcaagc gatgttcccc gccgcgcggc tcgagcccgc
     3241 gatcgtcgcc cctccggcgc tcgcggcgca gcactggtcg cgcgaggacg cgctggtcga
     3301 gatcctgcgc ggccggctcg aagggttggg gcccgtcacc gtcccggcgc tggccgcgcc
     3361 gctcggggtc gctgtcgatg cgatcgcggc ggcgctcgcc gcgctgcagt ccgagggctt
     3421 cgtcatgcgc ggccgcttca cgccgggcgt cgtcgaagac gaatggtgcg agcgc
//
LOCUS       DASYNS010000428         4108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4321484, whole genome shotgun sequence.
ACCESSION   DASYNS010000428 DASYNS010000000
VERSION     DASYNS010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4321484"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="VGP50_13290"
     CDS             <1..961
                     /locus_tag="VGP50_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007399604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV8028401.1"
                     /translation="AGFTENDVATSILDTLSGSFQTSPIFFLNLTNGVNYNLAAQTPQ
                     YKVQSLQDLENISITSPTAAHPGVLADVASISRSQEMAVVNHYNIRRVIDIYASVQGR
                     DLGAAGGEVDRIVAADRKLLPRGSFVSIRGQLQTMQTSYVNLLSGLAFSIVLVYLLIV
                     VNFQSWVDPFIIITALPAALAGIVLCLFFTHTTLSVPALMGAIMCMGVATANSILVVS
                     FGRERLTEHGDAVKAAIEAGFTRFRPVLMTALAMIIGMVPMALGLGEGGEQNAPLGRA
                     VIGGLLLATVATLVFVPAVFALLHKRRPPGTLPAAAAPGYRPS"
     gene            984..2204
                     /locus_tag="VGP50_13295"
     CDS             984..2204
                     /locus_tag="VGP50_13295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV8028402.1"
                     /translation="MPPDDLRETERAPPLLRTLVRPEIRRRRWVAPAVAAAVVLALGL
                     GIYAGIRARAATEAELARETDAAAVPVVDVIRPKPGAPDQSLVLPGNTQAFSDTAIYA
                     RTNGYLKRWYFDIGAHVKRGQLLAEIDTPEVDDQLRQARADLATAQATLKLAAITADR
                     TEVLLKTRSVSTQERDNAVGSLNSDTAIVQSREAAVAQLEQLQSFEKVEAPFDGIVTA
                     RNIDVGALIAAGGAAPARELFHMVATDTLRIYVSVPETYSPAMHADATPKVTLDEYPG
                     QTFDGILVRTSNAIDPASRTLLVEVDVKNDDGRLLPGAYTSVHFTLPGAGRTVTIPAN
                     TLLFRKEGLQVGVLRGDKVELVPVRIGRDYGDIVEIVDGLQPGDEVVLNPADSLASGT
                     AVRVNRQKDAVAAP"
     gene            2201..3718
                     /locus_tag="VGP50_13300"
     CDS             2201..3718
                     /locus_tag="VGP50_13300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEV8028403.1"
                     /translation="MKGGWLRVVGALWALLAGCTVGPDYKQPAVATPSAYKEVPPTGD
                     EAAGIWRPARPEDGMLRGNWWEVFGDGTLDALEDQVTAANQTLKQAEANFRQARALIG
                     LQQANLYPTLAVEPSIQSLKDSGHQPYFVLKHPTPTGEYQLPLDLSYEVDLWGQVRRT
                     ITAAREEAQATSADLATASLSLHAELAIDYVELRSADAQQKLLDDTVAAFADALKLTE
                     DRLAGGGASEADVAQARTQLETTRVQATDVAVGRAQFEHAIAVLIGKPPADFSLPPVP
                     LSLDPPAVPVGVPSELLERRPDIAAAERRVAEANEQIGIAEAAFYPSLTLSAIGGFIG
                     TTATNWFEWQSVAWAVGGTISQTLFDGGRRHAQSEATVAAYDETIAAYRQTTLTAFQE
                     VEDNLAALQILAREAEQQKGAVAAAENSLQIFTNRYIGGLDPYLQVVTAQSIALANER
                     NDVDIRRRRLEADVLLIKGLGGGWSTAEIPALTGVNPHALDITLPGMGSGVVPTE"
     gene            3824..>4108
                     /locus_tag="VGP50_13305"
     CDS             3824..>4108
                     /locus_tag="VGP50_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEV8028404.1"
                     /translation="MPTRNVVLTKRQEKLIETLVESGRYQNASEVLRDGLRLVEQREA
                     EESAKLTVLREAACVGFAALDRGAFKEFENIEDLQSYLNGLSEKVITRTAE"
BASE COUNT          689 a         1395 c         1378 g          646 t
ORIGIN      
        1 ggcgggcttc accgagaacg atgtcgcgac cagcatcctc gacacgctga gcggcagctt
       61 tcagacgtcg ccgatcttct tcctgaacct caccaacggc gtcaactaca acctcgcggc
      121 gcaaaccccg caatacaagg tgcagtcgtt gcaggacctg gagaacattt cgatcacctc
      181 gccgacggcc gcgcatccgg gggtgctcgc cgacgtcgcc tcgatctcgc gctcgcagga
      241 aatggccgtg gtcaaccact acaacatacg ccgcgtgatc gacatttatg cctcggtgca
      301 ggggcgcgat ctcggcgccg ccggcggcga ggtcgaccgc atcgtcgccg ccgaccggaa
      361 gctgctgccg cgcggcagtt tcgtcagcat ccgcggccag ctccagacga tgcagacctc
      421 ctacgtcaac ctcctgagcg ggctcgcctt ctcgatcgtc ctcgtctatc tcctcatcgt
      481 cgtcaacttc caatcctggg ttgatccgtt catcatcatc acggcgctgc cggcggcgct
      541 tgccgggatc gtgctatgct tgttcttcac gcacacgacg ctgagcgtgc cggccctcat
      601 gggggccatc atgtgcatgg gcgtcgcgac cgcgaacagc atcctcgtcg tctccttcgg
      661 ccgcgagcgg ctgaccgagc atggcgacgc ggtcaaggcg gcgatcgaag caggcttcac
      721 gcgcttccgt ccggtgctga tgacggcgct cgccatgatc atcggcatgg tgccgatggc
      781 cctgggcctg ggcgagggcg gcgagcagaa cgcgccgctc ggacgcgcgg tgatcggcgg
      841 cctgctgctg gccaccgtcg caacgctggt gttcgtgccc gcggtcttcg cattgctgca
      901 caagcgccgg ccgcccggca cgctgccggc ggccgcggca cccggttatc gcccttcctg
      961 acgcatccgc ggagaagctc gccatgccgc ccgacgatct tcgagagacg gaacgcgccc
     1021 cgccgctgct gcgcaccctg gtgcgaccgg aaatccggcg ccgtcgctgg gtcgcgccgg
     1081 cggtggcggc agcggtggtg ctggcgctcg gtctcggcat ctacgccggt atccgggcgc
     1141 gcgccgccac cgaggccgag ctggcgcggg agaccgacgc ggcggcggtg ccggtggtcg
     1201 acgtcatccg tccgaagcct ggcgcgcccg accagtcgct ggtcctcccc ggcaacaccc
     1261 aggccttcag cgacaccgcg atctacgccc gcacgaacgg ctatctgaag cgctggtact
     1321 tcgacatcgg cgcccatgtg aagcgcggcc agttgctggc ggagatcgac acgcccgagg
     1381 tcgacgacca gctgcgccag gcgcgcgccg acctcgccac ggcccaagcc acgctgaagc
     1441 tcgcggcgat caccgcggac cggaccgaag tcctgttgaa gacgcgatcg gtctcgaccc
     1501 aggagcgcga caacgcggtg ggttcgctca attccgacac ggcgatcgtg cagtcgcgcg
     1561 aagccgccgt ggcgcagctc gagcagctgc aatccttcga gaaggtcgag gcacctttcg
     1621 acggcatcgt gacggcgcgc aacatcgatg tcggcgcgct gatcgccgcc ggcggtgccg
     1681 cgcccgcgcg cgagctcttc cacatggtgg cgaccgatac gctcaggatc tatgtctccg
     1741 tcccggaaac ctactcgccg gcgatgcatg cggacgccac ccccaaggtg acgctcgacg
     1801 agtatcccgg ccagaccttc gatgggatcc tcgtgcgcac ctccaacgcc atcgatcccg
     1861 cctcgcgcac gcttctggtc gaggtcgacg tcaagaatga cgacggacgg ctgttgcccg
     1921 gcgcctacac ctccgtgcat ttcacgctgc ctggggccgg gcggacggtg acgatcccgg
     1981 ccaacaccct gctgttccgc aaggaaggat tgcaggtggg cgtgctccgg ggcgacaagg
     2041 tggagctggt gccggtgagg atcggccgcg actacggcga tatcgtcgag atcgtcgacg
     2101 ggctgcagcc cggcgacgag gtcgtgctca acccggcgga ttcgctggcc agcggcaccg
     2161 ccgtccgcgt gaaccgccag aaggacgccg tagcagctcc atgaagggcg gatggttgcg
     2221 cgtcgtcggc gcgctctggg cgctgttggc cggctgcacg gtcggtcccg actacaagca
     2281 gccggcggtg gcgacgccgt cggcctacaa ggaggtgccg ccgacgggag acgaggcggc
     2341 cggcatctgg cggccggcgc ggcccgagga cgggatgctg cgcggcaatt ggtgggaggt
     2401 cttcggcgac ggcacgctcg acgcgctcga agaccaggtc accgcggcga accagaccct
     2461 gaagcaggct gaggccaatt tccgccaggc gcgcgccttg atcggcttgc agcaggccaa
     2521 tctatatccg acgctcgcgg tcgagcccag catacagtcg ctgaaggatt cgggacatca
     2581 accttatttc gtcctcaagc atccgacgcc cacgggcgag taccagttgc cgctcgatct
     2641 ctcctatgag gtcgatctct gggggcaggt gcggcgcacc atcaccgccg cccgcgagga
     2701 agcgcaagcg acatccgccg atctcgcgac ggccagcctc agcttgcatg ccgagctcgc
     2761 catcgactat gtcgagctgc gcagcgccga tgcgcagcag aagctgctgg acgacacggt
     2821 cgcggctttt gccgacgcgc tgaagctcac cgaggatcgc cttgccggcg gcggcgcttc
     2881 cgaggccgat gtcgcccagg cgcgcacgca gctcgagacc acgcgcgttc aagccaccga
     2941 cgtcgcggtc gggcgcgctc aattcgagca tgcgatcgcc gtgctcatcg gcaagccgcc
     3001 cgccgatttc agcctgccgc cggtgccgct gtcgctcgat ccgccggcgg tgccggtcgg
     3061 cgtcccctcg gagctgctgg agcgccggcc cgacatcgcg gccgccgagc gccgcgtcgc
     3121 cgaggcgaat gagcagatcg gcattgccga agcggcgttc tatccgtcgc tgacgctgag
     3181 cgccatcggc ggcttcatcg gcaccacggc gaccaactgg ttcgaatggc agagcgtggc
     3241 gtgggccgtg ggcggcacga tcagccagac gctgttcgat ggcggacggc gccacgcgca
     3301 atccgaggcc acggttgcgg cctatgatga gacgatcgcc gcctatcgcc agacgacgct
     3361 cacggcgttt caggaggtcg aggacaatct cgcggcgctg cagatcctcg cgcgcgaggc
     3421 cgagcagcag aagggggcgg tggcggcggc ggagaactcg ctgcagatct tcacaaaccg
     3481 ctatatcggc gggctcgatc cctatttgca ggtggtgacc gcgcaatcca tcgccctggc
     3541 gaacgagcgc aatgacgtcg atatcaggcg gcggcgcctg gaagcggatg tcctgctgat
     3601 caaggggctg ggcggcggct ggagcacggc cgagatcccg gcgctgaccg gcgtcaaccc
     3661 gcacgccctc gacattacgc tgccgggaat gggcagcggc gtcgtgccga cggaataatt
     3721 gaccaacgcg tgctgcggcc tctttccggg gacgggactt gcgccggaaa tggctatggc
     3781 ataatatgcc atagccatcg catgaaactt tgggacgatg ccgatgccga ccagaaatgt
     3841 cgtgctgacg aagcgccagg aaaagctcat cgagaccttg gtcgagtccg gccggtacca
     3901 gaacgccagc gaggtgctcc gcgacggatt gcggctggtg gagcagcgcg aggccgagga
     3961 aagtgcgaag ctgacagtcc tccgggaagc ggcttgcgtc ggcttcgccg cattggaccg
     4021 gggcgcgttc aaggaattcg agaatatcga ggacttgcag agctatctga atggcctctc
     4081 ggagaaggtc attaccagaa ccgccgaa
//
LOCUS       DASYNS010000429         8822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2293275, whole genome shotgun sequence.
ACCESSION   DASYNS010000429 DASYNS010000000
VERSION     DASYNS010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2293275"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VGP50_13310"
     CDS             complement(<1..575)
                     /locus_tag="VGP50_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEV8028405.1"
                     /translation="MSETSSSANAARSAKRRVVILGSTGSVGRNTVELVAADPDAYEV
                     EALVAQRSVDRLAQQARQLGAKLAVIGDETRYRALKALLDGTGIEVAAGAAAIVEAAS
                     RPADWVMAAIVGAAGLAPTLAAVQRGALVALANKEVMVCAGALVTAEARRCGARLLPV
                     DSEHNAIFQVFDVDEAHAIERIMLTASGGPFR"
     gene            complement(680..1387)
                     /locus_tag="VGP50_13315"
     CDS             complement(680..1387)
                     /locus_tag="VGP50_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEV8028406.1"
                     /translation="MSDSAPSFRLTREAILGDSIRAMVKALDPDALLLSPEEHRASLA
                     TLFANRPDKGDVRIFAYGSLMWNPLIHYVEKRIGLVRGHHRCFCLWSRIGRGTAQKPG
                     LTLALERGGSCRGVVYRVAESLAAQELEVIWGREMLTGAYAPRWLEVTTDEGPVHALG
                     FLINRAGVRYAGKLPEERIIAAIAEAHGPLGACATYLFNTVAHLEELGICERKLARLR
                     DLVRARIDAAALATASS"
     gene            1532..2782
                     /locus_tag="VGP50_13320"
     CDS             1532..2782
                     /locus_tag="VGP50_13320"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV8028407.1"
                     /translation="MMRKTLLAWAFGLATIAVAARAAEAPAEIKLGTLYASSGRYASI
                     SMPVHLGLKLWVDQKNAEGGAFIKAFNKKIPLKLVVYDDQSNTGTAATLYNQLITQDK
                     VDVLISDSGSVLTSVAVPIARDNKMLLIDQTGTGAAFFTPDNPYIVLIADPVSSIWPK
                     PLADFLTHDGPPLGIKRVAMIYATNDFTGTQATAVRRFIGEAKSSGVELVYDNGVPTD
                     TANYAVLINNIRAANPDAVIHLGYAPNDIAFLRSLADSGGRFKMLFCIYPGLETELLE
                     KNVGDTGLNGIYTYVTASEIAYQTEWGMSLADFKKAWDAKYADGKVEFGFNSVAGYTT
                     GLVVEKALAVADSLDQLALRKAIFSVSGKLKTLDGTFALDENGAQIGEITPLGQLVSD
                     GGHIKFVTLWPPDVATGKPLYPAP"
     gene            2921..3844
                     /locus_tag="VGP50_13325"
     CDS             2921..3844
                     /locus_tag="VGP50_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8028408.1"
                     /translation="MLTYTLVAGLLFGLYFSLVGLGLNLVFGVMRIVNLAHGDFLMLG
                     AFLAFWLFKLLAFNPLVTIPVAFALFLLLGLPLYYLLVPRLLSARDPEMLSIILFFGV
                     SQVIEAVTTIAFGTSERSIPGMALGNAVKWAASVIGVGYEPGPVAILGQSFPISWVMS
                     GLAGALAVLGIYLYLYCTRLGYLTRAVMVSREEAISTGIDVHRVSAIAFGIGIALAAI
                     AGIFAPFMLNIITPAMGVDATITSFAVIVIGSLGNPLGTVLGGVIYGVSLIFLQQSSY
                     SSWANTLPYLLLIGVLLARPSGLLGRQTRRA"
     gene            3837..4757
                     /locus_tag="VGP50_13330"
     CDS             3837..4757
                     /locus_tag="VGP50_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8028409.1"
                     /translation="MPRLVRHTLLILVPVVAALGVAPFVYGNHLLLFNFIVFMALAQG
                     VNIIYGFTGYLPFGYVGFFGAGAYGFSLVVMHLHTSPFAALLAGGLAAVLLGLLLTPL
                     FRLSGAYFSIANLAAALALLQIVSNPTLEGITKGPYGVSLSGVFNPTLAYNTALAILA
                     ATLFLVIYLRNSRFGMALQAIREDAISAAMAGIDVVRARIIAWLLAALIAGLTGAVFA
                     WYISVFYPETVFSGEVSIFAIVFALFGGTATILGPVLGVLILYGVYNAIGISTPQYFQ
                     LIYGLLIMGLVLFLPNGLVSLLRRWGIDVP"
     gene            4747..5481
                     /locus_tag="VGP50_13335"
     CDS             4747..5481
                     /locus_tag="VGP50_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028410.1"
                     /translation="MCPEAAPLLAVDGLVKRFGGFRALDGLSFHLKPGEILGLVGPNG
                     SGKTTCINVVSGLYAPDGGSLRFGGRAIGGLASHRLVHLGINRTFQVPRPFLTLTVAE
                     NVAVAEAYGGASRQEAMPVGEILSSLDLAAVAGRRAGELNSAQQKMLDLARALATRPR
                     LLLVDELAAGLNPAELERVAARLKELARSGIALLVVEHLMGFIDQITDRVIVMNAGKE
                     IFEGVMADAVRDKRVVEVFLGGEDAG"
     gene            5471..6184
                     /locus_tag="VGP50_13340"
     CDS             5471..6184
                     /locus_tag="VGP50_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8028411.1"
                     /translation="MRAETMLEAQALETGYGRVQVLWGVDLQVQEHETVVLLGANGAG
                     KTTLLKTLLGLLPAWRGAIRFRAEDITRLRTDHRVRRGMVYMSEMAVFPGMTVDENIR
                     IGGQFLDDAALRTRAAELYAVFPALAERRRALASSLSGGQRKMLGIAKALAGNPRLLV
                     MDEPSSGLSPLFVKEVIRVLADFRNHGLSLLVAEQNVKFLEIADRVYTLDGGRVGFTG
                     SVAAMHENDALRRAYFGLK"
     gene            6419..6634
                     /locus_tag="VGP50_13345"
     CDS             6419..6634
                     /locus_tag="VGP50_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028412.1"
                     /translation="MLDQEKLKQLSTHAFAAFGYSEIAYVKRVEADGFVAYVVHAADG
                     SYLCEFFDRAAAFATLREHHFEPCSIH"
     gene            6640..6837
                     /locus_tag="VGP50_13350"
     CDS             6640..6837
                     /locus_tag="VGP50_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028413.1"
                     /translation="MTIRRTSEQPAYAAARRKLARSHVVRDFAADWQRWTPAERVTAS
                     LLMGTITLAVAAVYAAQIFPY"
     gene            complement(6941..7369)
                     /locus_tag="VGP50_13355"
     CDS             complement(6941..7369)
                     /locus_tag="VGP50_13355"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV8028414.1"
                     /translation="MSAADNKKLMEEIFARVADRDSSLFVESLAENVTWCVTGRNSWS
                     RTFEGKSSVLGDLMGHFRSLLVERPRTVAQRFIADGDYVVVEARGDNLTKTGMRYDNE
                     YCLVYRLEDGKIVQIREYCDSALVDAALGPFPASRIPSAG"
     gene            7461..8384
                     /locus_tag="VGP50_13360"
     CDS             7461..8384
                     /locus_tag="VGP50_13360"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV8028415.1"
                     /translation="MNLSAVDLNLLVVLDALLLEANVSRAAMRIGLSQPAASHALQRL
                     RAVLGDPLLVRVGARMELTPRAQALRGPLTQALEQVRGLFVPDEFDAARSERRFRLMM
                     PDLAVELLVPALMERVTALAPNLRVDIVPWRGPAIMTAEFARTLDLVISIGDAFKGFH
                     RRLLYTDSDALATRRGHPDGKRLSRRDVFEKARHVAVVIRGQSEDLIDDWLRSKGIER
                     RIALVVPGYIDALHIAARTDLVAFVPRRLIAALAAPLSLATVEPPFDPGIDQQFMFYP
                     TRAQFDPGSIWLRNLVRDTGRALERPKRKAG"
     gene            8590..>8822
                     /locus_tag="VGP50_13365"
     CDS             8590..>8822
                     /locus_tag="VGP50_13365"
                     /inference="COORDINATES: protein motif:HMM:NF024230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV8028416.1"
                     /translation="MLTPVSRRKHMPFAAIGFAAVVSMVPGVYLFRMASGLLQIAAGS
                     ETTLELLGATAADVLTAAIVVLAMSLGLVVPKLV"
BASE COUNT         1370 a         3047 c         2789 g         1616 t
ORIGIN      
        1 cggaaagggc cgccgctcgc cgtgagcatg atgcgctcga tcgcatgcgc ttcgtcgaca
       61 tcgaagacct ggaagatggc gttgtgctcg ctgtcgacgg gaaggagccg cgcgccgcag
      121 cggcgcgcct cggcggtgac gagcgcgccg gcgcagacca tcacctcctt gttggcgagc
      181 gccaccagcg caccgcgctg caccgcggcc agcgtcggcg cgagcccggc cgcgccgacg
      241 atggccgcca tcacccaatc ggcaggccgc gacgccgctt cgacgatcgc cgccgcaccg
      301 gcagcgacct cgatgccggt gccgtcgagc agcgccttga gggcacggta gcgcgtctcg
      361 tcgccgatca cggcgagctt cgcgccgagc tggcgcgcct gctgcgccag gcgatcgacg
      421 ctccgctgcg ccaccagcgc ctcgacctca taggcatcgg gatccgccgc cacgagctcg
      481 accgtgttgc ggccgacgga gccggtcgag ccgagaatga cgacgcgccg cttcgcgctg
      541 cgggcagcat tggcagaaga actggtctcg ctcatcgagc ctctgatctc ccgagagccg
      601 ccgtcgccgg cagcccgttg ccccttgtcc tattcaaaaa gcgccggcgg cgccagagtc
      661 aaggttgccg caacctaatt taggagctcg ccgtcgccag ggcggccgcg tcgatccgcg
      721 cccgcaccag atcgcggagc cgggcgagct tccgctcgca gatgcccagc tcctcgagat
      781 gggcgacggt gttgaagaga taggtggcgc aggcgccgag cggcccatgc gcctcggcaa
      841 tggcggcgat gatgcgctcc tcggggagct ttccggcata gcggacaccg gcgcggttga
      901 tgaggaagcc caaggcatgg accggccctt cgtccgtggt cacctcgagc cagcgcggcg
      961 cataggcgcc ggtgagcatc tcgcgccccc agatgacttc gagctcctgc gccgcgagcg
     1021 attcggcaac gcgatagacg acgccgcggc aggagccgcc gcgctccaag gccagcgtca
     1081 agccgggctt ctgcgccgtg ccgcggccga tgcgcgacca caggcagaag cagcgatgat
     1141 gtccgcggac gaggccgatg cgcttctcga catagtggat caagggattc cacatgaggg
     1201 agccataggc aaagatccgg acgtccccct tgtccggacg gttggcgaag agcgtcgcaa
     1261 gcgaggcgcg atgctcttcc gggctcagca gcagggcatc gggatcgagc gccttcacca
     1321 tcgcacgaat cgaatcgccg aggatggcct cgcgggtcag gcggaaggac ggcgcgctgt
     1381 cgctcattcg cgccatgata gagaagatac gagcgggttt gtccagtcgg caccgcccct
     1441 cgcgttgaca cccccccgac ccatggttaa ggtcggcgcc aaaatcgcca gcgaaatcac
     1501 ggcgaaatca cgctaagacg gggaacgtcc gatgatgcgc aaaacgctgc tcgcgtgggc
     1561 tttcggcttg gcaaccatcg ctgttgcggc gcgcgcggcc gaagctccgg cggagatcaa
     1621 gctcgggacg ctctacgcct cgtcggggcg ctatgccagc atctcgatgc cggtgcatct
     1681 ggggttgaag ctctgggtcg atcagaagaa tgccgagggc ggcgctttca tcaaagcctt
     1741 caacaagaag atcccgctga agctcgtcgt ctacgacgac cagagcaaca cgggaacggc
     1801 ggccacgctc tacaaccagc tcatcacgca ggacaaggtc gacgtgctga tctcggattc
     1861 gggctcggtg cttacctccg tcgcggtgcc gatcgcgcgc gacaacaaga tgctgctcat
     1921 cgaccagacc ggcacgggcg ccgccttctt cacgcccgac aatccctata tcgtgctgat
     1981 cgccgatccc gtctccagta tctggccgaa gccgctcgcc gatttcctga cgcatgacgg
     2041 gccgccgctc ggcatcaagc gcgtggcgat gatctatgcg acgaacgact tcacggggac
     2101 gcaggcaacc gcggtgcgcc gcttcatcgg cgaggcgaaa tcctcgggcg tcgagctggt
     2161 ctatgacaac ggcgtgccga cggacacggc gaactacgcg gtgctcatca acaacatccg
     2221 cgccgccaat cccgatgccg tgatccatct gggctatgcg cccaacgaca tcgctttcct
     2281 gcgcagcctc gccgacagcg gcggcaggtt caagatgctg ttctgcatct atcccggcct
     2341 cgagaccgag ttgctcgaga agaatgtcgg cgataccggg ctcaacggca tctacaccta
     2401 cgtcaccgcg tccgagatcg cgtaccagac cgagtggggc atgagcctcg ccgacttcaa
     2461 gaaggcctgg gacgccaaat acgccgacgg caaggtcgag ttcggtttca actccgtggc
     2521 gggctacacc acggggctcg tggtggagaa ggcgctcgcc gtcgccgaca gcctcgacca
     2581 gctggcgctg cgcaaggcga tcttcagcgt ctccggcaag ctcaagacgc tcgacggcac
     2641 cttcgcgctc gacgagaacg gcgcgcagat cggggagatc acgcccttgg gccagctcgt
     2701 ctcggacggc ggccacatca agttcgtcac cctctggcca cccgacgtcg ccacgggcaa
     2761 acccctctac ccggcacctt gatcatcttt tggcggcact gaccccccac cctagccctc
     2821 cccctcaagg ggggagggaa ttgcagcgaa caatctgccg aaccccctcc ccccttgagg
     2881 gggagggccg gggtgggggg tgccgcgcca cgatctctcg atgctgacct acacgctggt
     2941 cgcggggttg ctgttcgggc tttacttcag cctggtcggg ctcgggctca atctcgtctt
     3001 cggcgtgatg cgcatcgtca atctggcgca tggcgatttc ctgatgctgg gggcgttcct
     3061 ggcgttctgg ctgttcaagc tcctcgcctt caatccgctc gtcaccattc cggtcgcgtt
     3121 cgcgctgttc ctgctgctgg gactgccgct ctactatctg ctggtgccgc gactcctttc
     3181 ggcgcgcgat ccggagatgc tgtccatcat cctgttcttc ggcgtctcgc aagtgatcga
     3241 ggccgtcacc accatcgcct tcggcaccag cgagcggtcg atcccgggga tggcgctggg
     3301 caatgcggtg aaatgggcgg cgagcgtcat cggcgtcggc tatgagccgg ggccggtcgc
     3361 gatcctcggc cagtcctttc cgatctcctg ggtgatgagc gggctcgccg gcgcgctcgc
     3421 ggtgcttggc atctacctct atctctattg cacgcggctc ggatatctga cgcgcgccgt
     3481 gatggtgagc cgcgaggagg cgatcagcac cggcatcgac gtccatcgcg tctcggccat
     3541 cgccttcggc atcggcatcg cgcttgccgc catcgccggc atcttcgcgc ccttcatgct
     3601 caacatcatc acgccggcca tgggcgtcga cgccacgatc acgtcctttg ccgtcatcgt
     3661 catcggctcg ctgggcaatc ccttagggac cgtgctgggc ggcgtcatct atggcgtgtc
     3721 gctgatcttc ctgcagcaga gcagctacag ctcctgggcc aacactctcc cctatctcct
     3781 gcttatcggc gtgctgctgg cgcggccgag cggcctcctc ggccgccaga cccgccgtgc
     3841 ctaggctcgt ccgccacacg ctcctcatcc tcgtgccggt ggtcgcggcg ctgggcgtgg
     3901 cgcccttcgt ctacggcaat cacctgctgc tcttcaattt catcgtcttc atggcgctgg
     3961 cgcagggcgt gaacatcatc tacggcttca ctgggtacct gcccttcggc tatgtcggct
     4021 tcttcggcgc aggcgcctat ggtttctcgc tggtggtgat gcatctccac acatcgcctt
     4081 tcgcggcgct gttggcgggc gggctcgccg cggtgctgct ggggctgctg ctgacgccgc
     4141 tcttccgcct ctcgggcgct tatttctcga tcgccaatct agcggcggcg ctcgccttgc
     4201 tgcagatcgt gtcgaatccg acgctcgagg gcatcaccaa ggggccttat ggcgtgagcc
     4261 ttagcggcgt cttcaacccg acgctcgcct acaacaccgc gctcgcgatc ctcgcggcga
     4321 cgctctttct ggttatctat cttaggaact cccgcttcgg catggcgctc caggcgatcc
     4381 gcgaggacgc gatcagcgcg gccatggccg gcatcgacgt cgtgcgcgcg cggatcatcg
     4441 cctggctgct ggcggcgctc atcgccggcc tcaccggcgc cgtcttcgcc tggtacatct
     4501 cggtcttcta ccccgagacg gtgttcagcg gcgaggtcag catcttcgcc atcgtcttcg
     4561 cgctcttcgg cggaacggcg acgatcctcg gcccggtgct cggcgtgctc atcctctacg
     4621 gcgtctacaa cgccatcggc atctcgacgc cgcagtattt ccagctcatc tacgggctcc
     4681 tcatcatggg gctggtgctg ttcctgccca acgggctcgt ctcgctgctg cgccgctggg
     4741 gcatcgatgt gccctgaggc ggcgccgctc ctcgcggtcg acgggctggt gaagcgcttc
     4801 ggcggcttcc gcgcgctcga cgggttgagc tttcatctga agccgggcga gatcctgggc
     4861 ctcgtcggcc cgaacggctc gggcaagacc acctgcatca acgtcgtctc gggactctac
     4921 gcgcccgatg gcggcagcct gcgcttcggc gggcgcgcga tcggcgggct cgcctcgcac
     4981 cgcctcgtcc atctcggcat caaccgcacc ttccaggtgc cgcggccgtt cctgacgctg
     5041 acggtggcgg agaatgtcgc cgtcgcggag gcctatggcg gcgcatcgcg gcaagaggca
     5101 atgccggtcg gcgagatcct gtcgtcgctc gatctcgccg ccgtcgccgg gcgccgcgcc
     5161 ggcgagctca acagcgcgca gcagaagatg ctcgatctcg cccgcgcgct cgcgacgcgg
     5221 ccacgcctgc tgctggtcga cgagctggcg gcggggctga acccggccga gctcgagcgc
     5281 gtcgcggcac ggctcaagga gctcgcgcgc tccggtatcg cgctcctcgt cgtcgagcat
     5341 ctcatgggct tcatcgacca gatcaccgac cgcgtcatcg tcatgaatgc cggcaaggag
     5401 atcttcgaag gcgtaatggc cgatgcggtg cgcgacaagc gcgtcgtcga ggtctttctc
     5461 ggaggcgagg atgcgggctg agacgatgct ggaggcacag gcgctcgaga ccggctatgg
     5521 ccgcgtccag gtgctgtggg gcgtcgacct ccaggtgcag gagcatgaga cggtggtgct
     5581 gctcggcgcc aacggcgcgg gcaagacgac gctgctcaag acgctgctcg gactgctgcc
     5641 ggcttggcgc ggcgccatcc gcttccgcgc cgaggacatc acgcgcctca gaaccgacca
     5701 tcgcgtgcgg cgcggcatgg tctacatgtc ggagatggcc gtgttccccg gcatgacggt
     5761 cgacgagaac atccgcatcg gcggccaatt cctcgacgac gccgcgctcc ggacccgcgc
     5821 cgccgagctc tacgccgtgt tccccgcgct cgccgagcgg cgccgcgcgc tggcctcgag
     5881 cctctccggc ggacagcgca agatgctggg catcgccaag gcgcttgccg gcaatccgcg
     5941 actcctcgtg atggacgagc cgtcctcggg cctgtcgccg ctcttcgtca aggaagtaat
     6001 ccgcgtcctc gccgacttcc gcaatcacgg cctgtcgctg ctcgtcgccg agcagaacgt
     6061 gaaatttctc gagatcgccg accgcgtcta cacgctcgac ggcggccgcg tcggcttcac
     6121 cggcagcgtc gccgccatgc acgagaatga cgcgctgcgg cgcgcctatt tcggcctcaa
     6181 gtgagttcag gcggcgctcc attaacggga acgcaatcaa ttcgcactat tcttgttgtc
     6241 gaagcactcc caaatcgcgg cgaggttcga tatgagcagc gaaagcacgc tcaagaagat
     6301 gactcgcgaa ttcaccaagt cgtcgcaatc ggaacaacag gattcttccg accctcaatc
     6361 ggcggttaac caagcccatg ctacggttct tggcaacggc gggacggaag aggttgccat
     6421 gctcgaccag gagaaattga agcagctttc gacgcatgct tttgccgcct tcggctacag
     6481 cgagatcgcc tatgtgaagc gcgtcgaggc cgacggcttc gtcgcctatg tcgttcatgc
     6541 ggccgatggc agctatctct gcgagttctt cgaccgcgcc gccgctttcg cgacgctgcg
     6601 cgagcatcac ttcgaaccct gcagcatcca ctgatcatca tgacgatacg ccgcacctcc
     6661 gagcaacccg cctacgcggc agcgcgccgc aagctcgccc gctcgcacgt ggtgcgcgac
     6721 ttcgccgccg actggcagcg ctggaccccg gccgaacgcg tcaccgccag tctgctcatg
     6781 ggcacgatca cgctcgccgt cgccgccgtc tacgcagcgc agatcttccc gtactgagcg
     6841 cgcgcgacgc tcgcgcttcg gcgctccctg agtttcagct tccgcttctc ccgccaagtc
     6901 tctgccgtct ccctacccgt catttcggct gccgctgctc tcaaccggcg gacggtatcc
     6961 gtgaggccgg aaacgggccc agcgccgcat cgaccagcgc cgagtcgcaa tactcccgga
     7021 tctggacgat cttgccgtcc tcgagccggt agaccaggca gtactcgttg tcgtagcgca
     7081 tgccggtctt cgtcaggttg tcgccccgcg cctcgaccac gacatagtcg ccatcggcaa
     7141 tgaagcgctg cgcgaccgtc cgcggccgct cgaccagcag cgagcggaaa tggcccatca
     7201 gatcgccaag caccgagctc tttccctcga aggtgcgcga ccacgaattc cggccggtca
     7261 cgcaccaagt gacattctcc gccaggctct cgacgaacag gctgctgtcg cggtcggcga
     7321 cgcgggcaaa aatctcttcc atcaacttct tgttgtcggc ggcactcatg cggcttctcc
     7381 ggtcgtgggt tggcgaagcc attgtcgcgc tcggcggatc attcttccaa tcgatagtcc
     7441 atatgatgga tattcttccc atgaatttat ccgccgtcga tctcaacctt ctcgtcgtcc
     7501 tcgacgcgtt gctgctcgaa gccaatgtca gccgcgccgc catgcggatc ggcttgtcgc
     7561 agccggccgc gagccatgcg ctccagcgct tgcgcgccgt tcttggcgat cccttgctcg
     7621 ttcgtgtcgg cgcgcgcatg gaattgacgc cgcgcgccca ggcgctgcgc gggccgctga
     7681 cgcaggcgct tgagcaggtg cgcgggctct tcgttcccga tgaattcgac gccgcgcgga
     7741 gcgagcggcg ttttcgcctg atgatgcccg atcttgccgt cgagcttctg gtccccgcgc
     7801 tgatggagag ggtcaccgcg ctggcgccga accttcgcgt cgacatcgtg ccatggcgcg
     7861 gcccggcgat catgacggcc gagttcgcgc gcactctcga tctggtgatc agcatcggcg
     7921 atgccttcaa gggatttcac cggcggcttc tctataccga cagcgacgcg cttgccacac
     7981 gccgcggtca ccccgacggc aagagactga gtcggcgcga cgttttcgag aaggcgcggc
     8041 acgtcgccgt cgtcatccgc ggccagagcg aggacctgat cgacgattgg ctccgcagca
     8101 agggcataga gcggcggatc gcgctggtgg tgccgggcta tatcgatgcg ctgcatatcg
     8161 ccgcgcgcac cgatctcgtc gctttcgtgc cgcgccgcct cattgccgcg ctggcggcgc
     8221 cgctctcgct cgcgaccgtc gagccgccct ttgatccggg tatcgaccag cagttcatgt
     8281 tctacccgac acgggcgcag ttcgaccccg gctcgatttg gctgcgcaac ctcgtccgcg
     8341 atacgggtcg ggcgctggaa cggccgaagc gaaaggccgg ctgacccggc caaccaaagt
     8401 ttaggtctct tgtcctctcg gccagtcgcg acgcgccctg cgtataatgg agcggccttg
     8461 tcccaccggt aacatggtgt taccgccgcc tcttagggat gacaggctcc tgcatcggcc
     8521 gcacttgtcg gtgatttctg ccttggcgcg gcgggcggag cggtggtcgc ttgtgtcgcc
     8581 gtcgcgcttg tgctcacgcc cgtctcccgg cgaaagcaca tgccgtttgc cgccatcggc
     8641 ttcgccgcgg tggtgtcgat ggtgcccggc gtctatctct tccggatggc cagcggcctc
     8701 ctgcagattg cggccggctc ggagacgaca ttggagctgt tgggggcgac ggccgccgat
     8761 gtcctgaccg cggcgattgt cgtcttggcg atgagcctcg gcctggtcgt cccgaaactg
     8821 gt
//
LOCUS       DASYNS010000430         2339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3698643, whole genome shotgun sequence.
ACCESSION   DASYNS010000430 DASYNS010000000
VERSION     DASYNS010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3698643"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /gene="ppk1"
                     /locus_tag="VGP50_13370"
     CDS             <1..1449
                     /gene="ppk1"
                     /locus_tag="VGP50_13370"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEV8028417.1"
                     /translation="SDIEIEEEAHDLVLMFETALKRRRRGRIIRLTFDAGTPDRLRDF
                     VAREIGARPEDIAVVEDLVGLSDVRQLITADRPELLFPPYAPRFPERIRDFGGDCFAA
                     IRSKDFVVHHPYESFDVVVQFLRQAATDPNVIAIKQTLYRTSEDSPIVAALVAAAEAG
                     KSVTALIELKARFDEERNIGWARRLERAGAQVVYGFVDLKTHAKVSLVARREGDLLRS
                     YVHYGTGNYHPVTARIYTDLSFFSCEPALCRDAARLFNFITGYARPRALEKIAISPKG
                     IRTRLLELIEAETNHAGQGRPAAIWAKMNALVDRELIEALYRASSAGVTIALVIRGMC
                     CLRPGVPGLSEHISVKSVVGRFLEHARIVCFGAGHGLPSPDAAVFISSADWMPRNLDS
                     RVEALIPIENPTVHQQILGQIMTANLDDEAQSWMLRPDGRYERLRPDGSGRSAHVYFM
                     TNPSLSGRGSALTRLNGQAGGRVGHWARSVHD"
     gene            1442..>2339
                     /locus_tag="VGP50_13375"
     CDS             1442..>2339
                     /locus_tag="VGP50_13375"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HEV8028418.1"
                     /translation="MTEVGAAGRDSEVESPPAAPGRGIVACGVYREGRRVEDITIEQC
                     AGHAGRDGSMVWLGLCEPDDALMRSVQEQFGLHDLLVEDAHQAHQRPKVDVYGAVLFI
                     ALRTAQLVAGSIEYGETHLIVGPGFVISIRHGASTSYAPVRRRCEQSPEQLRLGGSFV
                     IYAILDFVADNYFPIIDKITDELEAIEDDIFSPVRRENIERIYRLRAGLLRMRRAVSP
                     MVEICSRLSRHDLPIVSAAILPYLNDVQDHVLQVNEAIVDLRERLAAAFEASLLLSAA
                     RQNEIVKKLASWAAILAVPTAVA"
BASE COUNT          376 a          809 c          744 g          410 t
ORIGIN      
        1 agcgacatcg agatcgaaga ggaagcgcat gatctcgtgc tcatgttcga gacggcgctg
       61 aaacgccgtc gccgcggccg gatcatccgt ctcaccttcg acgccggcac acccgaccgg
      121 ctccgcgact tcgtcgcgcg ggagatcggg gcgcgccccg aggatatcgc ggtggtcgag
      181 gatctcgtcg ggctctccga cgtccggcag ctcattaccg cggatcggcc cgagctgctt
      241 ttccctccct atgcgccgcg ctttcccgag cggatcaggg atttcggcgg cgattgcttc
      301 gccgcgatcc gctccaagga cttcgtcgtc catcatccct acgaatcctt cgacgtggtg
      361 gtgcagttcc tgcggcaggc cgcgaccgac ccgaacgtca ttgccatcaa gcagacgctc
      421 tatcggacct ccgaggactc gccgatcgtc gcggcgctgg tcgccgccgc cgaagcgggg
      481 aagtcggtga ccgcgctcat cgagctcaaa gcgcgcttcg acgaggagcg caatatcggc
      541 tgggcccggc gcctcgagcg cgcaggcgcc caggtcgtct acggcttcgt cgacttgaag
      601 acgcatgcca aggtctctct cgtcgcgcgt cgcgaaggcg acctgctgcg atcctacgtc
      661 cactacggca ccggcaatta ccaccccgtc acggcgcgta tctataccga tctctctttc
      721 ttcagctgcg agccggcgct ttgccgcgac gccgcgcgtc tcttcaactt catcaccggc
      781 tatgcccggc cacgcgcgct cgagaagatc gccatctccc ccaaaggaat caggacgcgt
      841 ctcctcgagc tgatcgaggc cgagaccaac catgccgggc agggccgccc agcggcgatc
      901 tgggcaaaaa tgaacgcgct cgtcgaccgc gagctcatcg aggctctcta ccgcgcctcg
      961 agcgccggcg tgacgatcgc gctcgtcatt cgcggcatgt gctgcctgag gcccggcgtg
     1021 cccggcttgt cggagcatat cagcgtcaag agcgtcgtgg gccggttcct cgagcatgcc
     1081 cgcatcgtct gcttcggcgc cggccatggt cttccgtcac cggacgcggc ggtgttcatc
     1141 tcctccgccg attggatgcc gcgcaacctc gacagccggg tcgaagcgct gatcccgatc
     1201 gagaacccca cggtgcacca gcagatcctg ggccagatca tgacggccaa tctcgatgac
     1261 gaggcgcaga gctggatgct gcgtcccgac gggcgctacg agcggctccg gccggatggc
     1321 agcgggcgca gcgcccatgt ctatttcatg acgaatccga gcctgtccgg ccgcggcagc
     1381 gccttgaccc ggctcaacgg ccaagccggc gggcgcgtcg gccactgggc gcgcagcgtc
     1441 catgactgag gtcggcgcgg ctggtcggga cagcgaagtt gaatcgccac ccgcggcgcc
     1501 gggacgcggc atcgtggcgt gcggcgtcta tcgcgagggc cgccgcgttg aggacatcac
     1561 catcgagcaa tgcgcgggcc atgccggacg cgacgggtcg atggtctggc tcggcctctg
     1621 tgaacccgac gacgcgttga tgcgctcggt tcaggaacaa ttcggcttgc acgacctcct
     1681 cgtcgaagac gcccatcagg ctcatcagcg cccgaaagtc gacgtctatg gcgcggtgct
     1741 ctttatcgcc ttgcggacgg cgcagctcgt cgcgggcagt atcgaatacg gcgagacgca
     1801 tctcatcgtc ggcccgggtt tcgtcatctc gatacggcat ggcgcgtcga cctcctacgc
     1861 gccggtgcgg cgtcgctgcg agcagtcgcc cgagcagctc cggctcggcg ggagcttcgt
     1921 gatctacgcc atcctcgatt tcgtcgccga caactatttt ccgatcatcg acaagatcac
     1981 cgacgagctc gaggcgatcg aggacgacat cttctccccg gtgcgccgcg agaacatcga
     2041 gcgcatctat cgcctgcgcg ccgggctctt gcggatgcgg cgcgcggtct cgccgatggt
     2101 cgagatctgc agccggttga gccggcatga cctgccgatc gtcagcgccg ccatcctgcc
     2161 ttatctgaac gatgtccagg accatgtcct gcaggtgaac gaggcgatcg tcgatctgcg
     2221 cgaaaggctg gccgccgcgt tcgaggcgag cttgctgctt tccgccgccc ggcagaacga
     2281 gatcgtcaag aagctggcga gctgggccgc catcctcgca gttccgaccg cggtcgccg
//
LOCUS       DASYNS010000431         5820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3542385, whole genome shotgun sequence.
ACCESSION   DASYNS010000431 DASYNS010000000
VERSION     DASYNS010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3542385"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..892
                     /gene="trpC"
                     /locus_tag="VGP50_13380"
     CDS             77..892
                     /gene="trpC"
                     /locus_tag="VGP50_13380"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV8028419.1"
                     /translation="MSGLLAEICAKKRAAIAAAQTARPLAMLEREAAAAASPRGFIAA
                     LERKLAAGSYGLIAEIKRASPSRGLIRADFAPAELAGAYAKGGAACLSVLTDEPYFQG
                     CAEHLTAARDAVTLPVLRKDFMLDPWQMAEARAIGADCVLLIMAALTDAEALALEAAA
                     RRWGMDVLVEVHDRDELARALRLEARLIGINNRNLSTLKVDLSVTEELAREIPPDRLP
                     VSESGLRTPADLARMARAGARCFLVGESLVAEADVEAVTRALLALPGGQRLRA"
     gene            889..1368
                     /gene="moaC"
                     /locus_tag="VGP50_13385"
     CDS             889..1368
                     /gene="moaC"
                     /locus_tag="VGP50_13385"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEV8028420.1"
                     /translation="MSGFTHFDEKGAARMVDVSGKAETERAATAGASVIMEKATLERI
                     MAGSVKKGDVLSVARLAGIMAAKRTPDLIPLCHPLALTSVTVELSCDPARNAVDIVAT
                     CKLTGRTGVEMEALTAAAVAALTVYDMCKALDRGMRIVDLRLLHKSGGKSGTYEAQV"
     gene            1365..2579
                     /gene="glp"
                     /locus_tag="VGP50_13390"
     CDS             1365..2579
                     /gene="glp"
                     /locus_tag="VGP50_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEV8028421.1"
                     /translation="MISVEEARRRLLAPLKPLGAEQIALSNAVGRVLAEDVAARRTQP
                     PFAVSAMDGYAVRAADVAEVPARLKVVGAVPAGQSYPGTLGRGQAVRIFTGAPLPDGA
                     DAIVIQEDTARDGDVVLVREAAPAGHYVRPAGLDFREGDLGLRAGRRLTARDIGLAAA
                     MNRPWLLLRRKPRIAILPTGDEVVMPGDPVGPNQIVSSNGLALAALVDQCGGIPVHLP
                     IAADDSRSLQRIAVGAEGVDFLVTTGGASVGEHDLVRDALAETGLALDFWKIAMRPGK
                     PLMVGRYRDTPMMGLPGNPVSSLVCGLLFLKPAIERLLGLDAVAATPSRARLAVPLPA
                     NDRRQDYLRAMLARAADGTLEARPFPRQDSSMMSLLARSDCLVIRQPHAPAAAAGQMV
                     EILPLDDSQGLF"
     gene            2721..3443
                     /gene="lexA"
                     /locus_tag="VGP50_13395"
     CDS             2721..3443
                     /gene="lexA"
                     /locus_tag="VGP50_13395"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEV8028422.1"
                     /translation="MLTRKQYELLVFINRRLSDGGVSPSFEEMKEALGLKSKSGIHRL
                     ISGLEERGFIRRLPHRARALEVVRLPEDTAISSVRGRSTGATERSRFSPTVIRGDFKA
                     ALPGTTTSSSDESVQLPLYGRIAAGTPIEALRDQSNTIGVPAGLLGNGEHYALEVAGD
                     SMVDAGILDGDTVLIQRCDVAESGQIVVALIDSNEVTLKRLRRRGDSIALEPANKDYE
                     TRIFGPDRVKVQGRLVGLMRRY"
     gene            3592..5715
                     /locus_tag="VGP50_13400"
     CDS             3592..5715
                     /locus_tag="VGP50_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028423.1"
                     /translation="MRRSRILIGAVAPVIVTACFLGASPAQAASSGCQLGNGIKHIFY
                     LQFDNVHFTRDNPNVPSDLEQIPNLLNFIEDNGTMLAIHTTPLISHTADDIITTLTGV
                     YGENHGQPVANSYGFFKANGSIGFASSFAYWTDPSPDGTPNMIDQAGKTAPAPWVPFT
                     RAGCNVGAFSTADIEFENVSTDITTVFGASSPEAAEAKANFDKAEADFEGISVHCALN
                     SPVCANGQPDALPDEPGGYVGFKGLFGNKFVAPAINNGSPSVNDINGIPVADDNGNLG
                     FPGFDPTPAQTLGYAAQMLENGVDVVFTYIEDAHDNQSGDGAFGPGETGYVQQLAQYN
                     EAFGKFFARLQADGITKQNTLFIITADENDHFVGKPGQPPNCDGVNIPCTYIRVPANC
                     NGDSIQCTQTNLGEVNIDIRQLLITPPFNNPTVFSVHDDDAPTVYINGNPGQTDAVTR
                     TLEQNVAQVKAFDPVVNANVPVTARLAGVAEMSFLHMITADPARTPTFTLFGNDDFFF
                     VAGSGAETPCTPLAACSDEQPSFNWNHGDFQSDIVHTWCSFVGPGVRTMGVNDFTFTD
                     HTDVRPTILSLAGLTDDYAHDGRVVFEILEDNVLPPAIQANQGLLSVLASSYKAINAP
                     VGPLALKTLQLSTAGLAGNDATFTQVTAQIQSITVTRDAIASQMIAILEGAAFANQPV
                     NQLQVIELIDQADALQFGTPVPSFP"
BASE COUNT          964 a         2090 c         1837 g          929 t
ORIGIN      
        1 aggccgcctc cgcgatcgac agcggcgccg cgcgccgcac cctcgagcgc ctcatcgcca
       61 tcaccaacgg caagccatga gcgggctcct tgccgagatc tgcgccaaga agcgggcggc
      121 gattgccgcg gcgcagacgg cgcgcccgct cgccatgctc gagcgcgagg cggcggccgc
      181 ggcgtcgccc cgcggcttca tcgcggcgct cgagcggaag ctggcagcgg gcagctatgg
      241 gctcatcgcc gagatcaagc gcgcatcgcc gagccgcggc ctgatccgcg ccgacttcgc
      301 gccggccgaa cttgcgggcg cctacgccaa aggcggcgcc gcctgtctct cggtgctcac
      361 cgacgaaccc tatttccagg gttgcgccga gcatctgacg gccgcgcgcg atgccgtcac
      421 gctgcctgtg ctgcgcaagg atttcatgct cgacccctgg cagatggcag aggcgcgggc
      481 gatcggcgcc gattgcgtgc tgctcatcat ggccgcgctc acggacgccg aggcgctcgc
      541 gctcgaagcc gccgcgcgac gctggggaat ggatgtgctg gtcgaggtcc acgaccgcga
      601 cgagctcgcc cgcgcactcc gcctcgaggc ccgcctcatc ggcataaata accgcaatct
      661 cagcacgctg aaggtggatc tttcggtgac ggaagagctc gccagggaga tcccgccgga
      721 ccgtttgccg gtgagcgaga gcggccttcg cacacccgcc gatctggcgc gcatggcgcg
      781 cgcgggcgcg cgttgcttcc tggtcggcga atcgctggtg gccgaagcgg atgtcgaggc
      841 ggtgacgcgc gccttgctgg cgctgccggg cggccagagg ctgcgcgcat gagcggcttc
      901 acgcatttcg acgagaaggg cgcggcgcgg atggtcgacg tctccggcaa ggccgagacg
      961 gagcgcgcgg cgaccgccgg cgccagcgtc atcatggaga aggcgacgct cgagcggatc
     1021 atggcgggca gcgtcaagaa aggcgacgtg ctgtcggtgg cgcggctcgc cggcatcatg
     1081 gcggccaagc gcacgccgga cctgatcccg ctctgccatc ccctcgccct gacgtcggtg
     1141 acggtcgagc tcagctgcga ccccgcccgc aacgccgtcg acatcgtcgc gacctgcaag
     1201 ctcacgggac gcaccggcgt cgagatggag gccctcaccg ccgccgccgt ggccgcgctc
     1261 acggtctatg acatgtgcaa ggcgctcgac cgcggcatgc gcatcgttga cctccgcctg
     1321 cttcataaat cgggcggtaa gtccggtact tacgaggcac aagtgtgatt tcggtagagg
     1381 aagcgcgacg ccgcctcttg gccccgctca agcccttggg cgcggagcag atcgcactca
     1441 gcaacgcggt cggccgcgtc ctcgccgagg atgtggcggc gcggcggacg cagccgccct
     1501 tcgccgtctc ggccatggac ggctatgccg tgcgcgccgc cgatgtcgct gaggtcccgg
     1561 cgcgcctcaa ggtggtcggc gccgttccgg ccggccagtc ctatcccggc acgctcggcc
     1621 gcggccaggc ggtgcgcatc ttcaccgggg cgccgctgcc tgacggcgcc gatgccatcg
     1681 tcatccagga ggacacggcg cgcgacggcg atgtcgtgct cgtccgcgaa gccgcgccgg
     1741 ccggtcacta tgtgcgcccc gccgggctcg atttccgcga aggggatctg ggcttgcgcg
     1801 cgggacgccg gctcacggcg cgcgacatcg gccttgccgc ggcgatgaac cggccgtggc
     1861 tgctgctgcg ccggaagccg cggatcgcga ttctgccgac cggtgacgag gtggtgatgc
     1921 cgggcgatcc cgtcgggccc aaccagatcg tgagctcgaa cgggctggcg ctggcggccc
     1981 tcgtcgatca gtgcggcggc attcccgtgc atctgcccat cgccgccgac gacagccgca
     2041 gcttgcagcg catcgccgtg ggcgccgagg gcgtggattt cctggtcacc accggcggcg
     2101 cctcggtcgg tgagcacgat ctggtgcgcg acgcgctggc cgagaccggc ctcgccctcg
     2161 acttctggaa gatcgccatg cggccgggca agccgctgat ggtcggacgt taccgtgaca
     2221 cgcccatgat gggcctcccc ggcaacccgg tgtcgagcct cgtctgcggc ctcctgttcc
     2281 tgaagccggc gatcgagcgg ttgctcggcc tcgacgccgt cgcggcgaca cccagccggg
     2341 cccggctcgc ggtcccgctg ccggccaatg accgccgtca ggattatctc cgcgcgatgc
     2401 ttgcccgcgc cgctgacggg acgctcgagg cccgcccctt cccacgccag gacagctcga
     2461 tgatgtcgct gctggcacgg tccgattgcc tggtgatccg ccagccccat gcgccggccg
     2521 ccgctgcggg ccagatggtc gagatcctcc ccctcgacga cagccagggc ctcttctaga
     2581 ggcgctcgcg acgcttaaga caccggtaag acaccggaga attgccgctt gacgctgccg
     2641 gcgaaccaaa ctagaacatc cacagttttg gttttgttcc agatgcgttc gttccagacg
     2701 cgagggcgcg gagaccacag atgctgacgc gcaaacagta cgagttgctg gtcttcatca
     2761 atcgtcgtct gtccgatggc ggcgtctctc cttctttcga ggaaatgaaa gaggcgttgg
     2821 gcctcaagtc caagtcgggc atccatcgcc tgatcagcgg actcgaggag cgcggcttca
     2881 tccgccgcct gccgcatcgt gcgcgcgcgc tcgaagtcgt gcggctcccc gaggacaccg
     2941 cgatcagcag cgttcgcggg cgcagcaccg gcgcgacgga gcgcagccga ttctcgccga
     3001 cggtgattcg cggcgacttc aaggcggcgc taccgggcac gacgaccagc agcagcgacg
     3061 agtcggtgca gctgccgctc tatggccgca ttgccgccgg cacgccgatc gaggccttgc
     3121 gcgatcagtc gaacaccatc ggcgtccccg ccggccttct cggcaacggc gagcattacg
     3181 ccctcgaagt ggccggcgac agcatggtcg acgcgggcat tctcgacggc gacacggtgc
     3241 tgatccagcg ctgcgatgtg gcagagagcg gccagatcgt ggtcgccctc atcgacagca
     3301 acgaagtcac gctgaagcgg ctgcgccggc gcggcgattc gatcgcgctc gagcccgcga
     3361 acaaggacta cgagacccgc atcttcggcc ccgaccgcgt caaggtgcag ggccgactcg
     3421 tcggcctcat gcggcggtat tagtccccgc gcccctctca ccttcgccga cggcgcattc
     3481 acgccggctt cacattgcgg tcatctgcgc gtaacgcccc tcacgcctaa tgcgcgccga
     3541 tagagggccc agcggccctc ggtcgactca cgacagatgg gggatcaccg catgaggcgt
     3601 tctcgaatcc tgatcggcgc ggttgcgccg gtgattgtca ccgcctgctt cctgggcgcg
     3661 agcccggcgc aggccgcgag ctccggctgc cagctgggaa atggcatcaa gcacatcttc
     3721 tatctccagt tcgacaacgt gcatttcacg cgtgacaacc cgaatgttcc ctcggacctc
     3781 gagcagattc ccaatctgct gaacttcatc gaggacaatg gcacgatgct tgccattcac
     3841 accacgccgc tgatctcgca taccgctgac gacatcatca cgacgctgac cggcgtctat
     3901 ggcgagaatc acggacagcc ggtggcgaac tcctacggct tcttcaaggc gaatgggagc
     3961 atcggattcg ccagttcgtt cgcttactgg acggacccct cgcccgacgg cacgccgaac
     4021 atgatcgacc aggcgggcaa gacggcgccg gcgccatggg tccccttcac ccgggccggc
     4081 tgcaatgtcg gcgccttctc gacggccgac atcgagttcg agaacgtctc caccgacatc
     4141 accaccgtgt tcggcgcgag ctcgcccgaa gcggctgagg cgaaagcaaa cttcgacaag
     4201 gcggaagccg acttcgaagg catttccgtc cattgcgcgc tgaacagccc ggtctgcgcc
     4261 aatggccagc ccgacgcgct gcccgacgag cccggcggct atgtcggctt caagggactc
     4321 ttcggcaaca agttcgtggc gccggcgatc aacaatggca gcccctcggt caacgacatc
     4381 aacggcattc ccgtcgccga cgacaacggc aacctgggct tccccggatt cgatccgacc
     4441 ccggcgcaga cccttggcta tgccgcgcag atgctcgaga acggcgtcga cgtggtcttc
     4501 acctatatcg aggacgccca cgacaaccaa agcggcgatg gcgccttcgg ccccggcgag
     4561 accggctacg tgcagcagct cgcgcagtac aacgaggcgt tcggcaagtt ctttgcccgc
     4621 ctccaggccg atggcatcac caagcagaat acgctcttca tcatcaccgc cgacgagaac
     4681 gatcatttcg tcggaaagcc gggacaaccg ccgaactgcg acggcgtgaa tatcccctgc
     4741 acctatatcc gcgtcccggc caactgcaac ggcgacagca tccaatgcac gcagaccaat
     4801 ctcggcgaag tcaatatcga catccgccag ctgctgatca cgccgccgtt caacaaccca
     4861 accgtcttct cggtgcatga cgacgacgcg ccgacggtct acatcaatgg caaccccggg
     4921 caaaccgacg ccgtgacccg gacgctcgag cagaacgtcg cgcaggtcaa ggccttcgat
     4981 cccgtggtca acgccaatgt gccggtcacc gcgcggctcg ccggagtggc ggaaatgtcc
     5041 ttcctccaca tgatcaccgc cgatccggcg cgcaccccga cgtttactct cttcggcaac
     5101 gacgacttct tcttcgtcgc cgggtcgggc gcggagacgc cctgcacgcc gctcgctgcc
     5161 tgctccgacg agcagccgag cttcaactgg aaccacggcg acttccagtc ggacatcgtc
     5221 cacacctggt gcagttttgt cggtccgggc gtgcgcacga tgggcgtcaa cgacttcact
     5281 tttaccgatc atacggacgt ccgcccgact atcctgagcc tcgccggcct caccgacgac
     5341 tacgcccatg acggccgcgt cgtcttcgag atcctcgagg acaatgtgct gccgcccgcc
     5401 atccaggcga atcagggcct gctttccgtc ctcgccagct cctacaaggc gatcaatgca
     5461 ccggtcggcc ccctcgcgct caagacgctg cagctctcga ccgccggcct tgccggcaat
     5521 gatgcgacct tcacccaggt gacggcgcag atccagagca tcacggtgac ccgcgatgcc
     5581 atcgcctcgc agatgatcgc catcctcgaa ggcgcggctt tcgccaacca gccggtcaat
     5641 cagctccagg tgattgagct gatcgatcag gccgacgcac tgcagttcgg gaccccggtc
     5701 ccatcattcc cataacctga ccccccccct gcctcgacgc ccgcccgggt ccacgcccgg
     5761 gcgggctttc tttttgcgcc cgccccgcgc tctctgggcc accttacgct ccggactcgc
//
LOCUS       DASYNS010000432         4763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_420748, whole genome shotgun sequence.
ACCESSION   DASYNS010000432 DASYNS010000000
VERSION     DASYNS010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420748"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VGP50_13405"
     CDS             complement(<1..950)
                     /locus_tag="VGP50_13405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEV8028424.1"
                     /translation="MDDAARAVTTDADRAREVLRSVFGYGEFRGQQGEIIGHVIAGGD
                     ALVLMPTGGGKSLCFQIPAIVRPGLGVVVSPLIALMKDQVDALRQAGVRAAYLNSTLD
                     AAEAAEIERDMRQGGLELVYVAPERLVTPRCLELLSRCRLALFAIDEAHCVSQWGHDF
                     RPEYRGLSILHERFPDVPRIALTATADGPTRSDIVERLKLGEARVFAAGFDRPNIRYR
                     VVAKDEAKAQLEAFLAAEHPGDAGIVYCLSRRRVEEIAAFLCTKGRVALPYHAGLDAG
                     TRERNQDRFLREEGVIMVATVAFGMGIDKPNVRFVAHLDAP"
     gene            complement(1422..1871)
                     /locus_tag="VGP50_13410"
     CDS             complement(1422..1871)
                     /locus_tag="VGP50_13410"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV8028425.1"
                     /translation="MAKEIERKFLVRGERWRGLGRRSRRIRQGYLRRDPERVVRVRIA
                     DDGAWLTIKGRPVGAERAEFEYAIPRADANDMLAHLCLPPVIEKTRHEITVDGLIWEI
                     DEFHHPNRGLVLAEVELPRADFPVTLPDWIGEEVTGEPEYYNQNMGL"
     gene            complement(1871..2719)
                     /locus_tag="VGP50_13415"
     CDS             complement(1871..2719)
                     /locus_tag="VGP50_13415"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV8028426.1"
                     /translation="MPVGQPAGAPAWRAIAAGDAWHVGEFTCRLGPEDRPFEERHDFV
                     SIAAVVEGSFQYRSDAGAALLYPGSFLLGNAGAAFECGHDHGTGDRCIAFKYAPALFE
                     EVAASVAGSHRFRFGTAMLPAIRALALPAVAAEARAQGAGSIAGDELALGFLETVLTT
                     LGGTTATVAAPPPRDQRRISRVLRHIEAHADQPLDLDALAAQAFMSKYHFLRTFRRVV
                     GVTPYHYLLDLRMRRAAVELCTTAAPIASVAFDAGFGDLSTFNGHFRDVFGASPSAFR
                     RAQRAA"
     gene            2846..3226
                     /locus_tag="VGP50_13420"
     CDS             2846..3226
                     /locus_tag="VGP50_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV8028427.1"
                     /translation="MINHVSIGVRNLAATKRFYDAALRPLGYACLSDGKESLGYGRDS
                     IAFWISVADRPVPADTASGLHFCITAPTRASVDAFHAAALRTGGRDNGKPGLRADYGP
                     DYYAAFVVDPDGYRIEAYCSRAEA"
     gene            3459..>4763
                     /locus_tag="VGP50_13425"
     CDS             3459..>4763
                     /locus_tag="VGP50_13425"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEV8028428.1"
                     /translation="MGAAVDPPPREIGPVLSDGIEFLHGFGTVDHDPDGVAQIVAQSL
                     CVVRRTGGQRYFQCHLDFPAERGELVACLGDRVVDHAELTRGWQHSAFALEDVPVPAE
                     LRLAFRDAQGAPVEGVRIRSVFLSDTWGRGQRKRLKCSMPFDRAVVVQDFDVFMCSCP
                     EWLKRGGVLGNTKEARLTDIWNGAPYQRMRGLFLEHRYGEVCREEVCPLLLGEIKTPE
                     PSPEVIAAVNDGHTVMAHGPNSIQHDVDRGCNLECVMCRDEKILPREENVERGLRDVQ
                     DAIALDSVIDLTWSGAGEIFAMKKIVQSLESDTYARRGIKLNLVTNLTYFNDKLWQRI
                     GHNRIGIITMSADGCSPAVYNSVRVGADWDAVAANMRLLAKIRRQGRVESIVWNYTVL
                     RRNVCDVAKAIRFAGELGFDRFRLIAQFGELSRTEGNIFESFD"
BASE COUNT          785 a         1629 c         1603 g          746 t
ORIGIN      
        1 ggtgcgtcga gatgggcgac gaagcgtacg ttcggcttgt cgatgcccat gccgaacgcc
       61 accgtcgcca ccatgatcac gccctcctcg cgcaggaagc ggtcctggtt gcgctcgcgc
      121 gtgccggcgt cgaggccggc atggtagggc agcgccacgc gtcctttcgt gcagagaaac
      181 gccgcgatct cctcgacgcg gcggcgcgag aggcaataga cgatgccggc atcgcccgga
      241 tgctcggcgg cgaggaaggc ctcgagctgc gccttggcct cgtccttggc gacgacgcga
      301 tagcggatgt tcgggcggtc gaagcccgcg gcgaagacac gcgcctcgcc cagcttcagc
      361 cgctcgacga tgtcgctgcg tgtcggcccg tcggcggtgg cggtgagcgc gatgcgcggc
      421 acgtccggga agcgctcatg caggatcgag aggccgcgat attcgggccg gaagtcatgg
      481 ccccattgcg agacgcaatg cgcctcgtcg atggcgaaga gcgcgagccg gcagcgcgaa
      541 aggagctcga ggcagcgcgg cgtcacgagc cgctcgggcg cgacatagac gagctcgagc
      601 ccgccctggc gcatgtcgcg ctcgatttcg gcggcttcgg ccgcgtcgag cgtcgagttg
      661 agataggccg cgcgcacgcc ggcctggcgc agcgcgtcga cctggtcctt catcagcgcg
      721 atcaacggcg agacgacgac gccgaggccg ggacggacga tcgccgggat ctggaagcag
      781 agcgacttgc cgccgcccgt cggcatcagc accagcgcat cgccgccggc gatgacatga
      841 ccgatgatct ccccctgctg cccgcggaac tcgccatagc cgaagacgga gcgcagaacc
      901 tcgcgcgcgc gatcggcatc cgtggtcacc gcccgcgctg catcatccat gatcttaaga
      961 ctctccgccg atccgcacgc gcgcatacta atcgcggcgc gcacgcgaag ccaccccgcg
     1021 cgacgcgaag cgtcgtcgca ggcgggcgcc agcggttgct ctcgcaaccc ggcgcgacgc
     1081 gaagcgtcgt cgcgcgagag cccgcacagt gcaggatcgg actcgtctcc ctctccgccc
     1141 ctcagggtgc ggagagggct ggggtgaggt gaggcgcgcg acggcacgaa ccggaatggc
     1201 ggagagaccc acctcatcct aaatccttct ccgccctgaa gggcggagaa ggacttgagc
     1261 cccgcgcgac gcgaagcgtt gtcgcgggcg ggcggcagcg gttgctcttg caacccggcg
     1321 cgacgcgaag cgtcgtcgcg cgagagcccg cacggtgcgg ctctcgcgat tgctgcgcaa
     1381 tcgctgtcgc tgtgtcggac gagcgcgctt cgcgcgctcg cctagagtcc catgttctga
     1441 ttataatact ccggctcccc cgtcacctct tcgccgatcc agtccggcag cgtcaccgga
     1501 aaatccgcgc gcggcagctc gacttccgcc agcacgaggc cgcggttggg gtggtgaaat
     1561 tcgtcgatct cccaaatgag gccgtcgacc gtgatctcgt gccgggtctt ctcgatcacc
     1621 ggcggcagac aaagatgcgc cagcatatcg ttcgcgtcgg cgcgcggaat cgcgtattcg
     1681 aattccgcgc gctccgcccc gaccggccgc cccttgatcg tgagccaggc gccgtcatcg
     1741 gcgatgcgga cacggacgac gcgctcgggg tcgcggcgga gatagccttg acgaatgcga
     1801 cgggagcgtc gaccgagccc gcgccagcgc tcgccgcgca cgaggaattt gcgctcgatc
     1861 tccttggcca tcaggccgct cgctgcgccc ggcggaacgc gctcggactc gcgccgaaga
     1921 cgtcgcggaa atgcccgttg aaggtcgaga ggtcgccgaa gccggcgtcg aacgcgacgc
     1981 tcgcgatcgg cgcggcggtg gtgcagagct cgacggccgc gcggcgcatg cgcaggtcga
     2041 gcaggtaatg atagggcgtc acgccgacaa cgcggcggaa cgtgcggaga aagtggtact
     2101 tgctcatgaa agcctgcgcc gcgagcgcgt cgagatcgag cggctggtcg gcgtgggcct
     2161 cgatatggcg gagcacgcgg ctgatccgtc gctggtcccg gggcggcggc gcggcgaccg
     2221 tcgccgtcgt gccgccgagc gtcgtcagca ccgtctcgag aaagccaagc gcgagctcgt
     2281 cgccggcgat gctgccggcg ccttgcgccc tcgcctcggc cgcgaccgcc ggcaatgcca
     2341 gcgcgcggat cgccggcagc atggccgtgc cgaagcggaa gcgatgcgag cccgcgacgc
     2401 tcgcggcgac ctcctcgaag agcgcgggcg cgtatttgaa agcaatgcag cgatcgcccg
     2461 tgccatggtc atggccgcat tcgaaagcgg cgccggcatt gccgagaaga aaggagccgg
     2521 gatagagcag cgccgcgccg gcatccgagc gatactggaa cgatccctcg acgacggcgg
     2581 cgatcgacac gaaatcgtgc cgctcctcga acgggcgatc ctccggtccg agccgacagg
     2641 tgaattcgcc gacatgccag gcatcgcctg cggcaatcgc gcgccacgcg ggcgcgcccg
     2701 cgggctgtcc gaccggcatt ttcgcgtccg ccccgccgac ctctctcatg gcgcaagaat
     2761 agcaactttc cccaagcggc gcatcggccg cttgcgtaat ttcgcgctca ggcggcgctt
     2821 gatgccctat cagaggagaa gctcgatgat caatcatgtc tcgatcggcg tgcgcaatct
     2881 cgccgcgacc aagcggttct acgacgcggc gctgcggccg ctcggctacg cgtgcctgag
     2941 cgacggcaag gagtcgctcg gctacggtcg cgattccatc gccttctgga tcagcgtcgc
     3001 cgaccgcccg gtaccggccg acacggcctc gggcctccat ttctgcatca ccgcgccgac
     3061 gcgcgcgagc gtcgacgcct ttcatgctgc ggcgcttcgc acgggcggac gcgacaacgg
     3121 caagccgggc ctccgcgccg attacggccc cgactactac gccgcgttcg tcgtcgatcc
     3181 cgacggctat cgcatcgagg cctattgcag ccgcgccgaa gcgtgaggct ccccttcacc
     3241 cttctttgat ccgacggccg cggcaaaact cgatcgcgtg agattgcaag tattcgttga
     3301 catccgcacc tgaactttcc acctgatgag cgaccgtggt cgaacgccag tggtcggcgc
     3361 tgcggcgatc ccgggcgaca tgccggcgta tttaggatca ttccgatcgt gcatgccagg
     3421 atcgccgacg cagcactcgt gccgagcctg gtggcgtgat gggtgcggcc gtcgatccgc
     3481 cgccacgcga gatcggcccg gtgctgagcg acggcatcga gtttctgcac ggcttcggca
     3541 ctgttgatca tgatccggac ggtgtcgccc agatcgtggc gcagagtctc tgcgtggtgc
     3601 ggcggacggg cggccagcgc tatttccaat gtcatctcga ttttcctgcc gagcgcggcg
     3661 agctcgtggc ctgtctcggg gaccgggtcg tcgaccacgc cgagctcacc cgaggctggc
     3721 agcattcggc cttcgcactc gaagacgtgc cggtgccggc cgagctgcgt ctcgcctttc
     3781 gcgacgccca aggcgcgccg gtcgaggggg tgcgcatccg ctccgtcttt ctcagcgaca
     3841 cctggggacg cggccagcgc aagcggctca aatgctccat gccgttcgac cgcgccgtcg
     3901 tcgtccagga tttcgacgtc ttcatgtgca gctgccccga atggctgaag cgcggcggcg
     3961 tcctcggcaa caccaaggaa gcgcggctca cggatatctg gaacggcgcg ccctatcagc
     4021 gcatgcgcgg tctcttcctc gagcatcgct atggcgaggt ctgccgcgag gaggtgtgtc
     4081 ccctgctcct cggcgagatc aagacgccgg agccttcgcc cgaggtcatc gccgcggtca
     4141 acgacggaca caccgtcatg gcgcatgggc cgaacagcat ccagcacgac gtcgatcgcg
     4201 gctgcaatct cgaatgcgtg atgtgccggg acgagaagat cctgccgcgc gaggagaacg
     4261 tcgagcgcgg gttgcgcgac gtccaggacg cgatcgcgct cgactcggtc atcgacctga
     4321 cctggtcggg cgccggcgag atttttgcga tgaagaagat cgtgcagtcg ctggagagcg
     4381 acacctacgc gcgccgcggc atcaagctca atctcgtgac caacctcacc tacttcaacg
     4441 acaagctctg gcagcggatc ggccacaacc gcatcggcat catcaccatg tccgccgacg
     4501 gctgctcgcc ggcggtctac aacagcgtcc gcgtcggtgc cgactgggac gccgtcgccg
     4561 ccaatatgcg cctgctggcg aagatcaggc gtcaaggccg cgtcgagtcg atcgtctgga
     4621 actataccgt gctgcgccgg aacgtctgcg acgtcgccaa ggccatccgc ttcgctggcg
     4681 agctcggctt cgaccggttc aggctcatcg cgcaattcgg cgagctcagc cggaccgaag
     4741 gcaacatctt cgagagcttc gat
//
LOCUS       DASYNS010000433         1722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2451134, whole genome shotgun sequence.
ACCESSION   DASYNS010000433 DASYNS010000000
VERSION     DASYNS010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2451134"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="VGP50_13430"
     CDS             <1..1332
                     /locus_tag="VGP50_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV8028429.1"
                     /translation="NSADPDGVHQVALQIEAAKAGGGWVFYRFSRIEMKDPVPKLSYA
                     KGFDAWQWAYGTGIYVDDVDTAYWKTALVYVIVGGLIVLAAGMLIFGIARSISRPLGI
                     ITERMSLLADGNLDVDVPFLGEPHEMGRLARALHVFKLSRRKAEELMTAQQAEQTAKL
                     RRQERLERLIGDFHQRTARVIETVATAAAQVQSHARNLADMADRSRSNIAAVSHAAVE
                     TTGNVQAVAGAAEELSAAVGDVNRRVVKSTDVANRAVSETERTTSTMRGLVEAAKRIG
                     TIVQVIQDIASQTNLLALNATIEAARAGDAGKGFAVVASEVKTLASQTTKATEEIEAQ
                     VGAIQNETERAVEAIANIANTVDEMSEISTAIATAMEEQGSTTQEIARNIGHVADSTK
                     DVSANITGVSSAAETTSHAANELQTASDALLSQAKVLETEMKGFLGEMRAA"
BASE COUNT          321 a          577 c          573 g          251 t
ORIGIN      
        1 aactctgccg atcccgacgg tgtccatcaa gtcgcgttgc agatcgaggc ggcgaaggcc
       61 ggcggcggct gggtgttcta tcgcttctcg cgcatcgaga tgaaggaccc ggtgccgaag
      121 ctatcttacg cgaaaggctt cgacgcctgg caatgggcgt atggcaccgg catctatgtc
      181 gacgacgtcg acacggctta ttggaagacg gcgcttgtct acgtcatcgt tggtggcctc
      241 atcgtgctgg cggcgggcat gctcatcttc ggcatcgcgc gcagcatcag ccggccgctc
      301 ggaatcatca ccgagcggat gagcctcctt gccgacggca atctcgatgt cgatgtgcct
      361 ttcctcggcg agccgcatga gatgggccgc ctcgcccgcg cgctccatgt cttcaagctg
      421 tcgcggcgca aggccgaaga gctgatgacg gcgcagcagg ccgagcagac ggcgaagctg
      481 cgccgccagg agcgcctcga gcgcctcatc ggcgatttcc atcagcgcac ggcgcgggtg
      541 atcgagacgg tcgcgaccgc tgcggcgcaa gtgcagtcgc atgcccgcaa tctggcggac
      601 atggccgacc gcagccgctc gaacattgcg gccgtcagtc acgcggccgt cgagaccacc
      661 ggcaacgtcc aggcggtggc cggcgcggcc gaggagctgt cagcggcggt cggcgatgtc
      721 aaccggcgcg tcgtcaagtc gaccgacgtc gccaaccgcg ccgtctccga gaccgagcgc
      781 accacctcca ctatgcgcgg gctcgtcgag gcagcgaagc gcatcggcac gatcgttcag
      841 gtcattcagg atatcgcctc gcagaccaac ctgctggcgc tcaacgccac catcgaggcg
      901 gcccgcgccg gcgacgccgg caagggcttc gccgtggtcg cgagcgaagt caagacgctg
      961 gccagccaga cgaccaaggc caccgaggag atcgaagcgc aggtcggcgc gatccagaac
     1021 gagacggagc gcgcggtcga ggccatcgcc aacatcgcga ataccgtcga cgaaatgagc
     1081 gagatcagca ccgccatcgc cacggcgatg gaggagcagg gctcgaccac gcaggagatc
     1141 gcgcgcaaca tcggccacgt cgccgacagc accaaggacg tgtcggccaa catcaccggc
     1201 gtcagctcgg cggccgagac gacgagccac gccgccaacg agctgcagac cgcgtccgac
     1261 gcgctgctca gccaagccaa ggtgctcgag accgagatga agggcttcct cggcgagatg
     1321 cgggcggcct gacgaagaga cggggggcaa gccgctcctc ctcagaaggc cgttacgtcg
     1381 ggctgagggc ccagcccctg acggccctgc atgaaccaaa cacgcgggtg cgcggggtca
     1441 actccgcgcc tcgcgcttcc gaagcgcgat ttgcctgcag gataggcaag cgggtgtcgc
     1501 cgcatttgac ggcgatatga caggccggcg cgccacaacc ggagggcttg cctcctcact
     1561 gtcacaaccg ctggcacatt tctcaacgga agcgggcggg ggctggcggc cgacccctta
     1621 ggggtagtct ctgccggacg agctagcgct ttattgaacg gcgtcgcctt ttcgctgcta
     1681 tccggcgacc cacgacagcg atatagtcgc cttcgccgcg ta
//
LOCUS       DASYNS010000434         3139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_889024, whole genome shotgun sequence.
ACCESSION   DASYNS010000434 DASYNS010000000
VERSION     DASYNS010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889024"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VGP50_13435"
     CDS             complement(<1..1223)
                     /locus_tag="VGP50_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8028430.1"
                     /translation="MADGSGNDERAEKLFEAAALCEQRGEIGSALDLYRRVIAMAPRR
                     AGAYMATANILKDQGKRAAAAAYLRRATIAIAPSAAAYADLGLYLCRLQQCDAAAEAI
                     ARSLAIEACRVGTLVNQGILLHKMGDIAGALRAYDQAIAVEPENPFARWNRGLTLLAE
                     GDLARGFADYEYRARIPGGGPRDPGIALWRGENLYRKTLIVHAEQGIGDTLQFLRYVP
                     FLAAAGARVVFGPQLELMALLKDFPGIAELAPPGSAPAADFHVPLMSLAHRFGTTLES
                     IPCRTPYLAPPAGLSATLTQPRGSKLRIGVVWAGRSAHQDDHARSMPFEELLALADLP
                     AATFYSLQIGERAQDIAACGAAGLIRDLSPQLTDVAVTAAVLRELDLVVTVDTAVAHL
                     AGAMARPCFVLLPFSA"
     gene            1447..2289
                     /locus_tag="VGP50_13440"
     CDS             1447..2289
                     /locus_tag="VGP50_13440"
                     /inference="COORDINATES: protein motif:HMM:NF021454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2167 domain-containing protein"
                     /protein_id="HEV8028431.1"
                     /translation="MSSGRAWCFGMILAAALGNPCAAIAASPQTDAAIKSLPFARGGN
                     FTLPISGGTLAVPARYYVLTGESAKTFYQLVNGLDAPKGLEAVMLDEGWKSLVLFTAE
                     LPGYLSLDDWNTLDAAAILQGVEARAARDNPERKSHGAQELTAIGWSQIPELDRTAHV
                     VRLSINAMAGDKPVLNNQQILLGRQGCEILEWIASSRAADDGLLVTARTAFSFAPDGE
                     YTDYRAGDGKAEYGVSEFLARAVGGKLPAGNGRFSWLAGRIALIVLVSIAGLWRASRR
                     QQIA"
     gene            complement(2332..>3139)
                     /locus_tag="VGP50_13445"
     CDS             complement(2332..>3139)
                     /locus_tag="VGP50_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028432.1"
                     /translation="HSNAFKRFDLGTGSESYPDYRPFVTDKETATGGTILGELRISKF
                     LIERIGEESPVLSFRPGHLSNPYALPQALAALGYRYSSSVTADASLTHLPFRLDYNRE
                     TAAETTVFEFPVTVEDEALPPLGERLPEALALADKIARYGGSFVVLIHPDILGHKFAF
                     EQGFVEALKGKAWFGALEDFGAWWAARNAIDLDVAESGGTEIVTLTLPQKMAGLTLAV
                     PGGWTYLSSDPAGLQASQAGSAIVLAEAQGTIKLRFAPHGAAADSDKLAH"
BASE COUNT          554 a         1052 c         1066 g          467 t
ORIGIN      
        1 gcagagaacg gcagaagcac gaagcaggga cgagccatcg cgccggcgag atgcgcaacc
       61 gccgtgtcga cggtcacgac gaggtcgagc tcgcgcagca ccgccgccgt caccgcgaca
      121 tcggtgagct gtggactcag atcgcgaatg aggccggcag cgccgcaggc cgcgatgtcc
      181 tgggcgcgct cgccgatctg gagactgtag aaggtcgccg ccgggagatc ggccagagcg
      241 aggagctcct cgaacggcat cgagcgcgca tgatcgtcct gatgcgccga acgtcccgcc
      301 cacacgacac cgatgcggag cttcgacccg cgcggctggg tgagcgtcgc cgagagtccg
      361 gcgggaggcg cgagataggg cgtcctgcac gggatgctct cgagcgtcgt gccgaagcga
      421 tgggcgaggc tcatgagcgg cacgtgaaag tcggcggcgg gcgccgagcc gggcggcgcc
      481 agttcggcga tgccgggaaa atctttcagc agcgccatca gctcgagctg cggaccgaag
      541 acgacgcgag cgcccgccgc cgcgagaaac ggcacgtagc ggagaaattg cagcgtgtcg
      601 ccgatgccct gctcggcatg aacgatcagc gtcttacgat agagattctc gccccgccag
      661 agcgcgatac cggggtcgcg cggaccccct ccgggaatcc gcgcgcggta ttcatagtcg
      721 gcaaagccgc gcgcgagatc gccctcggcg agcagcgtca ggccgcgatt ccagcgtgca
      781 aacggattct ccggctccac ggcgatcgcc tgatcatagg cccggagcgc gcccgcaatg
      841 tcgcccatct tatgcagaag gattccctgg ttgaccaggg tcccgacgcg gcacgcctcg
      901 atggcgagcg agcgggcgat cgcctcggcc gccgcgtcgc attgctgcaa gcggcacaga
      961 tagaggccga gatcggcata ggccgccgcc gacggcgcaa tcgctatggt cgcgcgccgc
     1021 agataggccg cggcagcagc acgcttgccc tggtctttca ggatattggc cgtggccatg
     1081 taggcgccgg cgcggcgcgg cgccatcgcg atcacgcggc gatagagatc gagcgctgaa
     1141 ccgatctcgc cgcgctgctc gcagagcgcc gccgcctcga aaagcttttc ggcccgctcg
     1201 tcgttgccgc tgccgtcagc catgcgctcg ctccgccgcg aagagcaagg atacaaccgc
     1261 cgggcacggc gcctcaaccg cgaccgcgcc ggcctgcgac accgttgcca gggaaacaca
     1321 cgcgctcgaa aagcgaccgc gagccctctc cggcgagggg ctgcattgcg cgccgccgac
     1381 cgacgttcac cgccgcttaa tcgttttttc ctgcaataga cgcctcaacc ggcgcaggag
     1441 acctgcatgt cgagcggtcg agcatggtgc tttggcatga tcctggcggc ggcgctcggc
     1501 aatccctgtg cggcaatcgc cgcctcgccc caaaccgacg cggccataaa gtccctcccc
     1561 tttgcgcgcg gcggcaactt caccctgccg atctccggcg gtaccttggc cgttcccgcg
     1621 cgatattacg tgctgaccgg cgagagcgcg aagacctttt accagctggt caacggcctc
     1681 gacgccccca agggcctcga ggcggtgatg ctggacgaag gatggaaatc actggtgctg
     1741 ttcacggcgg agctgcccgg ctatctcagc ctcgatgatt ggaacacgct cgatgccgcc
     1801 gcgatcttgc agggcgtaga ggcgcgcgcc gcgcgggaca atccggaacg caagtcgcac
     1861 ggcgcgcaag aactcaccgc catcgggtgg tcgcagattc ccgagctcga tcgcaccgcc
     1921 catgtcgtgc gcctcagcat caacgccatg gcgggcgaca agccagtgct caacaaccag
     1981 caaatcctgc tcggccgcca aggatgcgag atcctggaat ggatcgccag ctccagagcg
     2041 gcggatgacg gcctcctcgt caccgcgcgc accgcgttca gcttcgcgcc ggacggcgaa
     2101 tataccgact atcgcgccgg cgacggaaag gcggaatacg gcgtttccga gtttctcgcg
     2161 agagccgtcg gcggcaagct gccagccgga aacggccgtt tcagttggct cgcgggccgc
     2221 atcgcgttga tcgtgctcgt ctccattgcc ggcctatggc gcgccagccg acgccaacag
     2281 atcgcttgat cgaagcgaac cgcgggaacg acgaacgaaa gtgccgcagg ctcagtgcgc
     2341 gagcttgtcg ctgtcggcgg ccgcgccgtg cggcgcgaaa cgcagcttga tcgtgccttg
     2401 ggcttccgcc aggacgatcg ccgaaccggc ttgggatgcc tggagaccgg ccgggtcgct
     2461 cgacagatag gtccagccgc ccggcaccgc gagggtgagg ccggccatct tctgcgggag
     2521 ggtcaaggtc acgatctccg tgccgccgct ctcggcgacg tcgagatcga tggcgttgcg
     2581 cgcggcccac caggcgccga aatcctcgag ggcgccgaac cacgccttgc ctttcagcgc
     2641 ctcgacgaag ccctgctcga acgcgaattt gtggccgagg atgtcgggat ggatgagcac
     2701 gacgaaactg ccgccatagc gcgcgatctt gtcggcgagc gccagtgcct cagggagacg
     2761 ctcgcccagc ggcggcagcg cctcgtcctc gacggtcacc gggaattcga acaccgtcgt
     2821 ctccgcggcg gtttcgcggt tgtagtcgag ccgaaacggc agatgcgtga gcgaggcgtc
     2881 ggcggtcacc gaggagctgt agcggtagcc gagcgccgcg agtgcctgcg gcagcgcgta
     2941 gggattcgaa agatggcccg ggcggaagct gagcacgggc gactcctcgc cgatgcgctc
     3001 gatcaggaac ttgctgatgc gcagctcgcc gaggatggtg ccgcccgtcg cggtctcctt
     3061 gtccgtcacg aaggggcgat agtcgggata gctctcgctg cccgtcccga gatcgaagcg
     3121 cttgaaggca ttcgagtgc
//
LOCUS       DASYNS010000435         5072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1982558, whole genome shotgun sequence.
ACCESSION   DASYNS010000435 DASYNS010000000
VERSION     DASYNS010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982558"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..450
                     /locus_tag="VGP50_13450"
     CDS             193..450
                     /locus_tag="VGP50_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028433.1"
                     /translation="MVRRFTGPIGSLNDAWKAQRGVWVVCLDCGHAAKLDPRTLVAKL
                     GQELSDLPFDRLERRAKFTCRRCRRRRVGFLPHYMPWGAMR"
     gene            complement(497..709)
                     /locus_tag="VGP50_13455"
     CDS             complement(497..709)
                     /locus_tag="VGP50_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028434.1"
                     /translation="MAKRPSKETVAERLSAAELQVLERLIEKGALVTKPQQPNAKPRK
                     VMAAALSQDGRHIILWLSKNTIDLEG"
     gene            866..1438
                     /locus_tag="VGP50_13460"
     CDS             866..1438
                     /locus_tag="VGP50_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028435.1"
                     /translation="MITLNELTTGFSEALRVPLDEVQDVAERQHRAGLIAPVLAGQRH
                     MSAADGARLLVGLMVMRIEGAATPAAALTADIERLTRLKHGAQLYFDEDFILPEDYTD
                     AVAEILSALADPTRSARARDWIGRIGLARGAGRMAGWIEVRAAGAEPWEDFDYAASPD
                     DLVAILDQAPLVRRIEVRTAALLPIAQLLA"
     gene            1435..1641
                     /locus_tag="VGP50_13465"
     CDS             1435..1641
                     /locus_tag="VGP50_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028436.1"
                     /translation="MTTRLLSERERQEEADRFTRSLAGLAFAFLLMAIGLWLAEALAM
                     EAKLEDCMLQGRMNCERIELHPAP"
     gene            complement(1649..2485)
                     /locus_tag="VGP50_13470"
     CDS             complement(1649..2485)
                     /locus_tag="VGP50_13470"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEV8028437.1"
                     /translation="MLDLVTPVSGDGENGAPPVMLPGDVVGAPPGAGSAERRIDEWLG
                     IAAAALLHLAVILWLVVDWNLSIPPPQPEAVPVQLVMVPPEPAPPPQPQPKPTPKYRE
                     SGQDEQTTAPPSADQAAAEPTAPPASTPSTELPAPERAETTRDKDAPHAPRKEAAVAR
                     APRPDAQRPIEIEPGERDLQGDPYLNHLRALIARHWIVPKAASQLGLPLEGVAVYTII
                     IDPRGNLTSVALKKSSGTPALDAAGERMLREAAPFPPPPPDFPGIDWPIVWTVGLYSD
                     PP"
     gene            complement(2503..3645)
                     /locus_tag="VGP50_13475"
     CDS             complement(2503..3645)
                     /locus_tag="VGP50_13475"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV8028438.1"
                     /translation="MMLPLAGIKVVEIGQNLAGPYAGEVLATLGADVVKVERPEGDDA
                     RGWGPPFWRGTSAIFHTINRNKRSIALDLKDPSAVAWLRDYLAEVDVLVHNLRPGAMA
                     ELGLDAESLCARYPRLVYGALSAFGHKGPRRLQLGYEPLVQAFAGIFSVNGAEDGEPA
                     RVGVPILDMGTGMWLALGCIAALLRRTQTGRGGTVDASLFETALTWLTVAFSNFQVSG
                     QVPVRHRTGSRRLIVFEAFAATDGEIIIAAANDRLFRKLAVALGRPEWTEDERFTTNA
                     QRLAHKDVLLPEIRAIVAREPSEHWVELLEAAGVPCAPVQDVKHAVAEPQAAALGMIA
                     DVPESGLSLIGLPLSFDGERPPMRQRAPELGEHNDELRPAKGKAAE"
     gene            3751..4095
                     /gene="hspQ"
                     /locus_tag="VGP50_13480"
     CDS             3751..4095
                     /gene="hspQ"
                     /locus_tag="VGP50_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEV8028439.1"
                     /translation="MTTQRVHRAAKFSVGQIVRHRLYPFRGVIFDVDPIFNNTEEWYQ
                     SIPEDRRPSKDQPFYHLLAQNAQGGPYEAYVSEQNLLPDAENGPVKHPMIEVLFERRE
                     GDRYVLRASRQQ"
     gene            complement(4146..>5072)
                     /locus_tag="VGP50_13485"
     CDS             complement(4146..>5072)
                     /locus_tag="VGP50_13485"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV8028440.1"
                     /translation="IAQRDPRVRCLQRIGRRGLSTACIEGALASAAPYVAVMDADLQH
                     DERLLPRMLDALKHQPYDIVVGSRYIAGGGVGDWNKGRAQISDLATRLSRLICKTEIA
                     DPMSGFFMLRRDLFEASVRNLSGQGFKILLDLLASSPRPVRLKELPFEFRSRQHGESK
                     LDSLVAWEFGMLLADKLIGHLVPLRFALFAFIGIIGLGVHLAVLWTLHKGLGGEFTTA
                     QTVATFVAMTSNFFLNNVFTYRDQRLRGWRLLRGLFSFYLICAVGAVANVGIAGYIFA
                     SRPVWWLAGIAGVVIGSVWNYALSSIFTWRAR"
BASE COUNT          812 a         1677 c         1770 g          813 t
ORIGIN      
        1 aggagctgct ggcgctgttg cggccctggc ccgacgatcg catgcggatc tattccgtga
       61 gcccgcgcgt caacaatgtg aagaacgacg attcgggcct gatcgagccg ctgatggcag
      121 agggtggcac gctggtctaa gcgatcaatt gttgcgttga gcggcgccgc tccgcgttcc
      181 tagaatgttc tgatggtccg ccgtttcacc ggtccgatcg gtagcctcaa cgacgcctgg
      241 aaggcgcagc gcggggtctg ggtggtctgc ctcgattgcg gccatgccgc caagctcgat
      301 ccgcggacgc tcgtcgcgaa gctgggccag gagctgagcg atcttccgtt cgaccggctc
      361 gagcgccgcg ccaaattcac ctgccggcgc tgccgccgcc gccgcgtcgg cttcctgccg
      421 cattacatgc cctggggagc gatgagatag gaagaagtcc tgctctgccg cggacgaacg
      481 cccttcgggc gtccgcctag ccttcgaggt cgatcgtgtt cttcgagagc cagaggatga
      541 tgtgccggcc gtcctgggaa agcgcggccg ccatcacttt tcgcggcttg gcattgggct
      601 gctgcggctt cgtcaccagt gcgcctttct cgatcagccg ctccaggacc tgcagttccg
      661 ccgccgacag ccgctcggcc accgtctcct tgctcggtcg ctttgccatg agcgttgctc
      721 tcctgtcggt tcccccggcg catactaccg caggggccat ttgcgcgcgg gggttatggg
      781 ctcttgactc gtacggcgcc ccccttctgt gcaataatcg cgtcgagcct agcccaggcc
      841 gcgtcgcaac agaggcgcgc aagccatgat cacgctcaac gaactgacga ccggattcag
      901 tgaagcattg cgtgtgccgc tcgacgaggt gcaggacgtc gccgagcgcc aacaccgggc
      961 cgggctcatc gcgccggttc tggcggggca aaggcacatg agcgccgccg atggcgcgcg
     1021 tctcctcgtc ggcctgatgg tgatgcggat cgagggcgcg gcgacgcccg cggcggcgct
     1081 caccgccgat atcgagcgcc tcacgcgttt gaagcacggc gcgcagcttt atttcgacga
     1141 ggatttcatc ctgcccgagg attataccga cgccgtggcg gagattctct cggcgctcgc
     1201 cgatccgacg cggagcgcgc gcgcacggga ttggatcggc cgcatcgggc tcgcgcgtgg
     1261 cgccgggcgg atggccggct ggatcgaagt gcgcgcggcc ggggcggagc cgtgggagga
     1321 tttcgactac gcggcctcgc ccgatgatct ggtggcgatc ctcgaccagg cgccgctggt
     1381 gcggcgcatc gaagtgcgga ccgcggcgct tctgccgatc gcgcaactcc tcgcatgacg
     1441 acgcgcctcc tgagcgagcg cgagcgacag gaggaggcgg accgtttcac gcgcagcctc
     1501 gccggcctcg ctttcgcgtt cttgctcatg gctatcggcc tctggctcgc cgaggccctg
     1561 gcgatggaag cgaaactcga ggactgcatg cttcagggcc gcatgaactg cgagcgcatc
     1621 gagctgcatc ccgcgccctg agcgcgcgct acggcgggtc cgagtagagc ccgaccgtcc
     1681 agacgatcgg ccagtcgatg cctgggaaat cgggtggagg cggggggaag ggcgcggcct
     1741 cgcggagcat gcgttcgccc gccgcatcga gagcgggcgt tcccgatgat ttcttcagcg
     1801 cgacgctcgt caggtttccg cgcgggtcga tgatgatggt gtagacggcg acgccttcga
     1861 gcggcaggcc gagctggctc gcggccttgg gcacgatcca atgacgcgct atcaaagcgc
     1921 gcaggtgatt gagatagggg tcgccctgca ggtcgcgctc gccgggctcg atctcgatgg
     1981 ggcgttgcgc atcgggacgg ggcgctcggg cgacggccgc ttccttgcgc ggggcatgcg
     2041 gcgcgtcctt gtcccgcgtg gtctcggcac gctccggcgc gggcagttcg gtgctcgggg
     2101 tcgaggccgg cggggccgtg ggctccgccg ccgcctgatc ggcggaaggc ggcgcagtcg
     2161 tctgctcgtc ctggccggat tcgcgatatt tcggcgtggg ttttggctgc ggctgcggcg
     2221 gaggtgcggg ttcgggcggc accatgacca gctgcacggg cacggcttcc ggctggggcg
     2281 gcgggatcga gagattccag tccaccacca gccacaggat gaccgcgaga tgcagcagcg
     2341 ccgccgccgc aatgccgagc cattcgtcga tgcggcgctc cgcgctcccg gcgccaggtg
     2401 gcgcgccgac gacatcgccg ggcaacatga ccggcggcgc gccgttctcg ccgtcacccg
     2461 acaccggggt caccagatcc agcatcaagc gtgatacgcg cgctactccg ccgccttccc
     2521 cttggccggc cgcaattcgt cattgtgctc gccgagctcg ggcgcgcgct gccgcatggg
     2581 cgggcgctcg ccgtcgaaag agagcggcag gccgatcagg ctgaggccgc tctcgggcac
     2641 gtccgcgatc atgccgagcg ccgccgcttg cggctccgcc acggcatgct tcacgtcctg
     2701 caccggcgcg cagggcacgc ccgccgcctc gagcagctcc acccaatgct ccgagggctc
     2761 gcgggcgacg atcgcgcgga tctcgggcag cagcacgtcc ttgtgggcca ggcgctgcgc
     2821 attggtggtg aagcgctcgt cctcggtcca ttccggccga cccagcgcga cggcgagctt
     2881 gcggaagagc cggtcgttgg cggcggcgat gatgatctcg ccatccgtcg cggcgaaagc
     2941 ctcgaagacg atgaggcggc ggctgccggt gcggtggcgc acgggaacct ggccgctcac
     3001 ctggaaattc gagaaggcca cggtaagcca ggtgagcgcg gtctcgaaaa gcgaggcatc
     3061 gaccgtgccg ccgcgcccgg tctgcgtccg ccgcagcagc gcggcgatgc agcccagcgc
     3121 cagccacatc ccggtgccca tgtcgaggat gggaacgccg acgcgggccg gctccccgtc
     3181 ctcggcgcca ttgacgctga agatgccggc gaaggcctgc accagcggct catagccaag
     3241 ctgcagccgc cgcgggccct tgtggccgaa ggccgagagc gcgccataga cgagccgcgg
     3301 atagcgcgcg cagaggctct ccgcgtcgag gccgagctcg gccatcgcgc ccgggcgcag
     3361 attgtgcacg agcacatcga cctcggcgag gtaatccctg agccaagcca ccgcgctcgg
     3421 gtccttgagg tcgagcgcga tgctgcgctt gttgcggttg atggtgtgga agatcgccga
     3481 ggtcccccgc cagaagggcg gtccccagcc gcgcgcatcg tcgccctcgg ggcgctcgac
     3541 cttgacgaca tcggcgccca gcgtcgccag aacctctccg gcataggggc cggcgaggtt
     3601 ctgtccgatc tcgacgacct tgatgcccgc caatggcaac atcatgcatc tcctgcgccc
     3661 cggttgcgcc tcctcgcagc ggcttgagga ttgttgcgcg agtccatata agaaatttgc
     3721 gccgccggcg cgatactagc gaggagctcc ttgacgacac aacgcgtcca ccgcgcggcc
     3781 aagttctccg tcggccagat cgtccgccac cgcctctacc cgtttcgcgg cgtgatcttc
     3841 gacgtcgatc ccatcttcaa caacaccgag gaatggtatc agtcgatccc cgaggatcgc
     3901 cggccgtcca aggaccagcc tttctatcat ctcctggcgc agaacgcgca gggcggcccc
     3961 tacgaagcct atgtgtcgga gcagaacctg ctgcccgacg ccgagaacgg cccggtcaag
     4021 catccgatga tcgaggtgct cttcgagcgc cgcgagggcg accgctacgt gctgcgcgcc
     4081 agccggcagc agtgatcggg cgataatcaa cgactttccc tctcccacaa gggggagagg
     4141 gtttcttagc gcgcgcgcca ggtgaaaatc gaggagaggg cgtagttcca gacggagccg
     4201 atgacgacac cggcgatgcc ggcgagccac cacacgggac gcgaggcgaa gatgtagccg
     4261 gcgatgccga cattggcgac ggcgcccacg gcgcagatca gatagaagga aaacaggccg
     4321 cgcagcagcc gccatccccg aaggcgctgg tcgcgatagg tgaagacgtt gttgaggaag
     4381 aagttcgagg tcatcgccac gaaggtggcg acggtctggg ccgtggtgaa ctcgccgccg
     4441 aggcccttgt gcagggtcca cagcaccgcg agatggacgc cgaggccgat gatgccgatg
     4501 aaggcaaaaa gcgcgaaacg cagcggcacg agatggccga tgagcttgtc ggcgagcagc
     4561 atgccgaatt cccaggcgac cagcgagtcg agcttgctct cgccgtgctg gcgggaacgg
     4621 aattcgaagg gcagctcctt gagacgcacc ggccttggcg acgacgccag aaggtcgagc
     4681 aggatcttga agccttgtcc cgagagattg cgcacgctcg cttcgaagag atcgcgccgc
     4741 agcatgaaga agccgctcat gggatcggcg atctcggtct tgcagatgag gcgcgagagg
     4801 cgcgtggcga ggtcgctgat ctgagcgcgg cccttgttcc agtcgccgac gccgccgccc
     4861 gcgatataac ggctgccgac gacgatgtcg taaggctgat gcttgagcgc gtcgagcatc
     4921 cgcggcagga gacgctcgtc atgctgcaga tcggcatcca tgaccgcgac atagggcgcc
     4981 gccgaggcga gcgcgccttc gatgcaggcg gtcgagaggc cgcgccggcc gatgcgctgg
     5041 aggcagcgca cgcgcgggtc gcgttgcgcg at
//
LOCUS       DASYNS010000436         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_889779, whole genome shotgun sequence.
ACCESSION   DASYNS010000436 DASYNS010000000
VERSION     DASYNS010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889779"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VGP50_13490"
     CDS             complement(<1..609)
                     /locus_tag="VGP50_13490"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEV8028441.1"
                     /translation="MTATTALVRTMNPTDCDQIPPPLAAMAEFTNGSRLAPHQRKYAQ
                     LVHVRTGVLRVSTATGEWTVPPFRALWIPPETLHEIRMIGGVTTQTLDVEPHLAGELW
                     AECRVIEVRGLLKALLLQILDQPLDRASGERSGLMIKLVLHELKGAAPVPLHLPMPRD
                     QRLLKICRALIEHPESDDTLAGWAKRASASARTVARLFQAETG"
     gene            839..1585
                     /locus_tag="VGP50_13495"
     CDS             839..1585
                     /locus_tag="VGP50_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028442.1"
                     /translation="MTDLLARLNERDVAAFYRRFAQGIQLALGNDALAAALLLHWLDG
                     GGRPKAILGQDLRTLPEIRAYLRSTARAVLLSVKTLPGGMVDGIVPRLRGTIKANPPG
                     GPFQIRIDGNVDTVLSARAKAGKSMTVAEAELGALYALDGWTVTSDVVMSATPTTTPQ
                     QYDVKFDRWTCKVSAGYVWAGDKDMTVPNPDQGLSGKNAVAPGDKEITLYNKNAIRVE
                     NAGMANPFVYETDAWDETDIAVVGPAQVRV"
     gene            1651..2082
                     /locus_tag="VGP50_13500"
     CDS             1651..2082
                     /locus_tag="VGP50_13500"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEV8028443.1"
                     /translation="MIRGSCQCGGIRFEIDAVLAITHCHCSICRKLSGGAFASYAHVA
                     AGKFRFVSGEDLIASYESTPGSHRHFCRVCGSPAPGKASYLETVSIPAGLLDDDPGAR
                     PVLHTFVASKAPWFEITDELRQFEKWVPGHAPKGGSESRAP"
     gene            complement(2089..>2766)
                     /locus_tag="VGP50_13505"
     CDS             complement(2089..>2766)
                     /locus_tag="VGP50_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028444.1"
                     /translation="EAMEGLAPGRYIALSVEDDGTGMPPHVLSHVFEPFFTTKEPGKG
                     SGLGLSQVFGFAKQSGGHVEIESALGLGTTVTLYLPAVAAGAEEEAAPRPVAAAAMRR
                     ARRGTALVVEDDPDVLESAIVSLESLGYDVLTAENGPGALDVLRRKQKIDLLFSDVVM
                     PKGMTGIELAREARRLRPEVKVLLVSGYPRSVLEANEPLDDFVLIAKPYRLEELVQGL
                     RRASAGG"
BASE COUNT          470 a          925 c          907 g          464 t
ORIGIN      
        1 gccggtctcg gcctggaaga ggcgagcgac ggtgcgcgcg ctggcgctgg cgcgcttggc
       61 ccagcctgcg agcgtgtcgt cggattcggg atgctcgatc aaggcgcggc aaatcttcaa
      121 caggcgctga tcgcgcggca tcggcaggtg cagcggcacc ggtgctgcgc ctttgagctc
      181 gtgaaggaca agcttgatca tgagcccgct gcgctcgccc gacgcgcggt cgagcggctg
      241 gtcgaggatt tgcaagagga gcgctttgag caatcccctg acctcgatca cgcggcattc
      301 ggcccagagc tccccggcaa gatgcggctc gacatcgagc gtctgtgtcg tcacgccgcc
      361 gatcatgcgg atttcgtgca atgtctcggg cgggatccag agggcgcgaa acggcggcac
      421 tgtccactcc ccggtcgccg tgctgacgcg caggacgccg gtccggacat gaacgagctg
      481 cgcgtacttg cgctgatgcg gcgcgaggcg gctgccattc gtgaattccg ccatggcggc
      541 gagcggcggc gggatctggt cgcaatctgt cggattcatg gtccgcacaa gcgctgtcgt
      601 cgctgtcatc gcttcctcgt cgagaacgag agctgcgtgc cctcggaagg agcgcgccgc
      661 tccgatcgaa ttggaggacg ctccagcttt actatcgggc aagttgctca cgcgatgatg
      721 tgcgctaccg cacagcccgc gcagcgcaaa gcatgcctgt cgcgactcgc ggcgaatgaa
      781 ttggctctcg gcggaagagc cgggtatcat gggctaaccc attgattgcg cagcaggcat
      841 gaccgatctt ctggcgcgat tgaatgagcg cgacgtcgcc gccttctatc gccgctttgc
      901 ccaaggcatt cagctggctc tcggcaacga tgccctggcc gcggcgctgc tgctccattg
      961 gctcgatggc ggcgggaggc cgaaggcgat tctcggtcag gacctgcgca ctcttcccga
     1021 gatccgggca tatctgcgca gcacggcgcg cgccgttctc ttgagcgtca agacgctgcc
     1081 ggggggcatg gtggacggca tcgtcccgcg tctgcgcggg accatcaaag ccaatccgcc
     1141 gggcgggccc tttcagattc gcatcgacgg caatgtcgac acggtgctgt cggctcgcgc
     1201 caaggccggc aagagcatga ccgtcgccga ggccgagctc ggcgcgctct acgccctcga
     1261 cggctggacg gtgacctcgg acgtcgtcat gtcggcaacg ccgacgacga cgccgcagca
     1321 atacgacgtc aagttcgacc gctggacctg caaggtgagc gcgggctatg tctgggcggg
     1381 cgacaaggac atgacggtgc ccaatcccga tcagggcctc agcggcaaga acgccgtggc
     1441 acccggtgac aaggagatca ccctctacaa caagaacgcg atccgggtcg agaatgccgg
     1501 catggccaat cccttcgtct acgagaccga cgcctgggac gaaaccgaca tcgccgtcgt
     1561 cgggcccgcg caagtacgcg tctaaaaatc gcaattccgc tgcgcagacg cgacggccgc
     1621 tgtgacaggc tgtgacaggg agcatggcaa atgatccggg gaagctgcca atgtggggga
     1681 atccgcttcg agatcgatgc cgtcttggcg atcacgcatt gtcactgctc gatctgccgc
     1741 aagctcagcg gcggcgcttt tgcgagctat gctcatgtcg ccgccggcaa gttccgcttc
     1801 gtttccggcg aagacctgat cgcctcttac gagtcgacac ccggcagtca tcggcacttc
     1861 tgccgggtct gcggttcacc ggcgccggga aaggcgtctt acctcgagac ggtcagcatc
     1921 ccagcgggcc tgctcgacga cgatcccggc gcccggcccg tccttcatac cttcgtcgcc
     1981 tcgaaggcgc cctggttcga gatcaccgat gaattacgcc aattcgagaa atgggtgcca
     2041 ggccacgcgc cgaaaggcgg gtcggagtcg cgggcgccgt aaccgccttc agccgccggc
     2101 cgaggcgcgg cgcaggccct ggaccagctc ttcgagccga taaggcttgg cgatcagcac
     2161 gaaatcgtcg agcggctcat tggcctccag cacggagcgc ggatagcccg agaccagcag
     2221 caccttgacc tcgggacgga gcctccgcgc ctcgcgcgcg agctcgatcc cggtcatgcc
     2281 tttcggcatg acgacgtcgg agaagaggag atcgatcttc tgcttgcgcc gcagcacgtc
     2341 gagggcgccc ggcccgttct ccgccgtgag cacgtcgtag ccgagactct ccagcgacac
     2401 gatcgccgat tcgagcacgt ccgggtcgtc ctcgacgacg agcgcggtgc cgcgccgcgc
     2461 gcgccgcatc gccgccgccg ccaccggtcg cggcgccgct tcttcttcgg cgcccgccgc
     2521 gacggccggc agatagaggg tcaccgtcgt gccgaggccg agcgcgctct cgatctccac
     2581 atggccgccc gactgcttgg caaagccgaa gacctggctc aggccaagcc cgctcccctt
     2641 ccccggctcc ttggtcgtga aaaacggctc gaagacatgc gacagcacat ggggcggcat
     2701 tccggttccg tcgtcctcga cggacagcgc gatgtagcgt cccggggcca gcccttccat
     2761 ggcttc
//
LOCUS       DASYNS010000437        13068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1202047, whole genome shotgun sequence.
ACCESSION   DASYNS010000437 DASYNS010000000
VERSION     DASYNS010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 13068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13068
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202047"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..803)
                     /locus_tag="VGP50_13510"
     CDS             complement(144..803)
                     /locus_tag="VGP50_13510"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV8028445.1"
                     /translation="MEQRPGAESIDADESLRRCIATGALRPRVELLRFVVAPDGAIVP
                     DLAGRLPGRGLWLTAQRDIVAAAVTKRLFARAARRPVIVDDGLADRVEALLAKRCLDH
                     IGLARRAGRAVMGFTKVQAALQAGKAAVLVAAADGAADGRGKLKALAPSLPLVERLTS
                     AELGAAFGREHVVHAALLPGRLADAFLADMARLGGFRVAPPEPFAEVSEADKACLPER
                     D"
     gene            complement(813..2360)
                     /gene="nusA"
                     /locus_tag="VGP50_13515"
     CDS             complement(813..2360)
                     /gene="nusA"
                     /locus_tag="VGP50_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEV8028446.1"
                     /translation="MQPSASYARPELLQVADTVARDKGIDRDEVLQAMEQAIQKAGRS
                     KYGQEYDIRAEIDRKNGEIRLMRFREVADPVENEATQIPITEAKRFNPEAELGDFITD
                     PLPPIDFGRIAAQTAKQVIVQKVRDAERQRQYSEYKDRIGEIVNGLVKRVEFGNVVVD
                     LGRAEAILRRDELLPRESFRQGERVRSYIYDVRQEVRGPQIFLSRTHPQFMAKLFAQE
                     VPEIYDSIIEIRAVARDPGSRAKIAVISNDSGIDPVGACVGMRGSRVQAVVAELQGEK
                     IDIIPWSQDPATFVVNALAPAEVAKVVMDEEQRRIEVVVPDDQLSLAIGRRGQNVRLA
                     SQLTGWDIDILTEAEESERRTEEFRTRSKMFIDALDVDDVIAHLLVTEGFTSVEEVAF
                     VPVAELGGIEGFDEDIAGELQQRARTFLEEQNVKLEARRRELGVGDDVAALAGMTPAL
                     LVAIGEKGIKTLDDLADLAGDELVEMAQPAVKLDQEQANGIIMAARAHWFAEEGAGTP
                     SGAAAGE"
     gene            complement(2400..2885)
                     /gene="rimP"
                     /locus_tag="VGP50_13520"
     CDS             complement(2400..2885)
                     /gene="rimP"
                     /locus_tag="VGP50_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV8028447.1"
                     /translation="MDTTKIEAMIAPSLEAMGYRVVRVAVTGGRRATLQIMAERTDDQ
                     AMRVEDCTEISRTVSALLDVADPIAGAYLLEVSSPGIDRPLVRREDFARFAGFEAKVE
                     LSAPVDGRKRFRGRLLGVEGDDVRLLVDALPVALPITTVTRAKLVLTDELLAHTSPPR
                     D"
     gene            complement(3021..3734)
                     /gene="trmB"
                     /locus_tag="VGP50_13525"
     CDS             complement(3021..3734)
                     /gene="trmB"
                     /locus_tag="VGP50_13525"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV8028448.1"
                     /translation="MDAPHPRVVYGRRHGRKLRAAQRELLTSLLPQLRFDVPAEGLLE
                     PRRLFAAPVEAIWLELGFGAGEHLAAQAAAQPSRGLIGCEVFENGIVKLLAEVKQRGL
                     ANVRLFVDDARVLVQSLAAASVERIFILFPDPWPKQRHHKRRLVSAAMLDALAHVLSD
                     GGELRLATDDMEYLRVMLGGAAAHPAFEWLARRPDDWRQRPEDWPATRYERKALAAGR
                     RPAFLRFRRRPRACEARRV"
     gene            complement(3752..4918)
                     /gene="metK"
                     /locus_tag="VGP50_13530"
     CDS             complement(3752..4918)
                     /gene="metK"
                     /locus_tag="VGP50_13530"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV8028449.1"
                     /translation="MAKRNYVFASESVSEGHPDKVCDRISDAIVDTFLAADPQARVAC
                     ETLATTNRVVLAGEVRGPDSITKDLLVEVARHAIRDIGYEQDGFHWKHAEVSCYLHKQ
                     SADIAQGVDAAGNKDEGAGDQGIMFGYACRETESLMPAPIYYAHAILRSLAEARHSGA
                     APFLGPDAKSQVSLQYIDGQPVRATAVVVSTQHAASIDQDEVREIVRPHVLNVLPQGW
                     MCDEAHFYVNPTGRFVIGGPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRS
                     AAYAARYLAKNVVAAGLADRCTIQVSYAIGVARPLSVYIDTAGTARVDEEKLSQVLQD
                     LVDLSPRGIRTHLGLNKPIYARTAAYGHFGRTPEADGGFSWERLDLVTELRAAF"
     gene            complement(5079..5528)
                     /locus_tag="VGP50_13535"
     CDS             complement(5079..5528)
                     /locus_tag="VGP50_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8028450.1"
                     /translation="MNTRRRRRKSDKPNPVDVHVGSRVRLRRTLLGMSQEKLGEAIGL
                     TFQQVQKYERGANRIGASRLYDLSRVLDVPVSFFFDDMKPETAETGRQESDGGIEPTI
                     ARYEPDPTMRRETLELVRAYYRIPDAQIRRRLFDLTKAIANACTSET"
     gene            complement(5646..7304)
                     /gene="lnt"
                     /locus_tag="VGP50_13540"
     CDS             complement(5646..7304)
                     /gene="lnt"
                     /locus_tag="VGP50_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEV8028451.1"
                     /translation="MSDTSGVAFRAAGPLSPLQRAALWTAELSGWRRYAAAWLLGALA
                     AAPLSPLDGTPFLVICLTGPPILAVSFSGLVWLLDGSAGRFDAALLGWSFGFGFFVAG
                     LYWIGAALFVDISAFWWLLPFAVLVFPAGLAIFAGLAVLAAHEVSRRFGLLGTARILV
                     LAVAWAGSEWLRGHVLTGFPWNLVGYVWAGTFPGALAVLQVASLVGIYGLSLLTVTAA
                     ALPARLGDLESGRRGAAFVALLLIAVPAAGGAWRLAHAPHDMVPGVMLRLVQPSIPES
                     LKNDPQARAQNFQRLLSLSALPGTDKVTDVIWPEAAAPPLLERFAEERQAIAAVVPKG
                     GLLLTGTERAEPLQGWPPLHVWNSLVVLDDKGDIIATYDKAHLVPFGEYMPLRGIVPI
                     DKIVPSIGDFSSGAGPRTLALPGVPPVGPLICYEAIFPGAVIDDAARPQWLLNVTNDA
                     WYGFTSGPFQHLAITRVRAVEEGLPLIRAANNGISAVVDPYGRVVQRLPLDSIGVLDS
                     KLPRALPPTLYDHARDMAFLALSLSLLAIAATLTLTGGRHGSRA"
     gene            complement(7297..8205)
                     /locus_tag="VGP50_13545"
     CDS             complement(7297..8205)
                     /locus_tag="VGP50_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV8028452.1"
                     /translation="MADTSANRPPGEAEGPFQVLRNWLKYLTRPRNGETSLREELEEL
                     IEEHKEEQPIDPHERTLLVNILKLHELSAADIMVPRIDITAIEVDTPFAEAVKQMVEF
                     GHSRVPLYHETLDDVIGIVHIKDLLPYAADGRTVPLGKIARKVLFVAPSLPVLDLLLQ
                     MRLSRVHMALVVDEFGGIDGLVTIEDVIEEIVGEIEDEHDDADQPKLIERPDGTLIAD
                     ARTRIQALQERVPTRLLPPEQDEGIETLGGLVGALAGRVPGRGEVFKHPAGIEFEVLD
                     ADPRRIKRVRVRGLAPAPAAPPPGDV"
     gene            complement(8198..8680)
                     /gene="ybeY"
                     /locus_tag="VGP50_13550"
     CDS             complement(8198..8680)
                     /gene="ybeY"
                     /locus_tag="VGP50_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV8028453.1"
                     /translation="MDIAEACPLWRRNLGDVDALCLSAARAALAGAEVLHGPAELSLV
                     LGDDAMLRALNCRWREQDRATNVLSFPALDVALPEGAPRLLGDVVLAFETIATEAAAQ
                     GKPLADHLRHLVVHGVLHLLGLDHATPAEAAQMEAREIAVLAQLGVPDPYRPLGGSHG
                     "
     gene            complement(8811..9842)
                     /locus_tag="VGP50_13555"
     CDS             complement(8811..9842)
                     /locus_tag="VGP50_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV8028454.1"
                     /translation="MNAAHGESGAQGGRVQMQFDDNTLLPLLFGERDENLDRIERQLG
                     VSVVSRGNRLAISGPSARTSVARTAIEALYDQLKRGLEVDAAAVDAALRLAADRAAQP
                     GATSWGEGNEIRTRKRRIAPRSANQASYINAMREHPLVFGLGPAGTGKTYLAVATAVD
                     LMMSGGVERIILSRPAVEAGERLGFLPGDLREKVDPYLRPLYDALYDMLPGEQVMKRL
                     SSGEIEIAPLAFMRGRTLANAFVILDEAQNTTPVQMKMFLTRLGENARMAVTGDLSQI
                     DLPAGAHSGLRDAIDTLAGVEGVAMIEFTDSDVVRHPLVTRIVRAYDRREAKRESSSR
                     SKKSDDRRP"
     gene            complement(9839..11194)
                     /gene="miaB"
                     /locus_tag="VGP50_13560"
     CDS             complement(9839..11194)
                     /gene="miaB"
                     /locus_tag="VGP50_13560"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEV8028455.1"
                     /translation="MAKKLYIKTYGCQMNAYDSARMADALAPLGYALVDAAEEADMII
                     LNTCHIREKASEKVFSELGRLRPLKAGKGASSNRLILAVAGCVAQAEGAEILARAPYV
                     DIVFGPQTYHRLPQMVAQVMRSGVQVLDTDFPAEAKFDFLPETTIPRGVCAFLSVQEG
                     CDKFCTFCVVPYTRGAEFSRPASALLAEARQLVAGGVREITLLGQNVNAYQGAAPDSG
                     IWGLGQLIRALAAIPGLARLRYTTSHPLDVDDALLAAHREVPQLMPFLHLPVQSGSDG
                     VLRAMNRRHTADDYRRVIERLRAERADIAFSSDFIVGFPGESATDFAETLTLVRDIGY
                     AQAFSFKYSPRPGTPAASMPEQVPEAVKAERLAELQELLAAQQRGFNAAMVGRELPVL
                     FEKRGRHPGQLVGRSPYLQAVHATAPDHVLGALLPVRFVRAEPNSLAGELSAAVAEAS
                     A"
     gene            complement(11218..12066)
                     /locus_tag="VGP50_13565"
     CDS             complement(11218..12066)
                     /locus_tag="VGP50_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV8028456.1"
                     /translation="MSATLLRLGRLSLYLGLTLPLMPVQAAALLLKRPLARRLPRIYH
                     RWSARILGFHIKTGGERSAERPTLFIANHTSYLDIEILGALIEGSFVAKHEVARWPFF
                     GWLAKLQRTVFVDRRVRSTAMQRDAIRERLDAGDNLILFPEGTANDGNRVLPFKSALF
                     SVADYKGACGALAVQPVSLAYTRLDGIPLGRFYRPFFAWYGDMELAPHLWAMLGLGTV
                     EVDVTFHRAVTLAQFGSRKALAEHCHRVIAQGVARALAGRTDEPASPPVEAPQPTPAQ
                     EAIGAE"
     gene            complement(12070..12813)
                     /locus_tag="VGP50_13570"
     CDS             complement(12070..12813)
                     /locus_tag="VGP50_13570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEV8028457.1"
                     /translation="MADIDAAQALRYRVFYDEMGASPSAEMARIERDFDSFDGVCDHL
                     LVVDHARGSGAAAVVGTYRLIRRSAAAHHGSFYSAAEYDIAEMLAYPGEILELGRSCV
                     DASARNRPTMQLLWRGIAAYVFQYDIALMFGCASLPGTDVDALATSLSYLYHHHLAPQ
                     GLRPSALPERRVEMRRLPADVLDPARALGDLPPLIKGYLRLGGFVGDGAVIDHQFNTT
                     DVCVVVKTDLVTDKYYRHYERQARDGQAA"
     gene            complement(12926..>13068)
                     /locus_tag="VGP50_13575"
     CDS             complement(12926..>13068)
                     /locus_tag="VGP50_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007088438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEV8028458.1"
                     /translation="TGRVIEFRNEDIEKLQRDVAERLGYRLVDHRLELFAVPLAGKVE
                     RK"
BASE COUNT         2273 a         4311 c         4342 g         2142 t
ORIGIN      
        1 ccgtgaggga agctctggcg gacttggctg gtctcgatcg acttgcgcag ctcaagccgg
       61 ctgcccggcc gcacgaggct gagcggacgc ttctgttcgt gctcgctgtt gtcgctgtta
      121 tcggtcattc ttcctcgata ccgttagtct ctctctggga ggcaagcctt gtcggcctcc
      181 gaaacttcag caaaaggttc tgggggcgcc acgcggaacc cgccaagccg cgccatgtcg
      241 gccagaaaag cgtcggcaag acgccccggc aggagcgccg catgcacgac atgctcacgg
      301 ccgaaggccg cccctaactc ggcgctcgtc aggcgctcga cgagcggcag gctcggcgcc
      361 agtgccttga gcttgccgcg tccgtcggcc gcgccatccg ctgccgctac caacaccgcc
      421 gccttgccgg cctggagggc ggcctgcact ttagtgaaac ccatcaccgc ccggccggcg
      481 cggcgcgcca aaccgatgtg atcgaggcag cgcttcgcca atagagcttc aacgcgatcc
      541 gccaggccat cgtctacgat gaccggccgt cgcgccgccc gcgcgaagag ccgcttggtc
      601 actgccgcgg ccactatatc gcgctgcgct gtcaaccaca aaccccgacc ggggagtcgg
      661 cccgccagat ccggcacgat cgcaccgtcg ggcgcgacca cgaaccgcag caattccacc
      721 ctcgggcgaa gcgcgcccgt agcaatacag cgccgcagcg actcatccgc gtcgatcgac
      781 tcagcgcctg gccgttgctc gatcgccgta gcctactccc cggccgcggc gccgctcggc
      841 gttcccgcgc cctcttcggc aaaccaatgg gcgcgagccg ccatgatgat gccgttggcc
      901 tgctcctgat cgagcttcac tgccggctgc gccatctcga ccagttcgtc acccgcgagg
      961 tcggcaagat cgtcgagcgt cttgatgccc ttctcgccga tagcgacgag caacgcgggc
     1021 gtcattccgg cgagcgccgc gacatcatcg cccacgccca attcgcgccg acgcgcttcg
     1081 agtttcacat tctgctcctc taggaaggta cgcgcccgct gctggagctc gcccgcaata
     1141 tcctcatcga acccttcgat gccgccgagc tcggccacgg gcacgaacgc cacctcttcg
     1201 acggaggtaa atccctcagt cacgagcaga tgggcgatga cgtcgtcgac gtcgagcgca
     1261 tcgatgaaca tcttactgcg cgtgcggaac tcctcggtgc ggcgctcgct ttcctcggcc
     1321 tcggtcagga tgtcgatgtc ccagccagtg agctgcgacg ctaggcgcac attctgccca
     1381 cgccggccga ttgccagcga gagctggtca tcgggcacga caacctcgat gcgccgctgc
     1441 tcctcgtcca tcaccacctt ggcgacctcg gccggagcga gggcgttgac gacgaaggtc
     1501 gcggggtcct gggaccacgg aatgatgtcg atcttctctc cctgcagctc ggcgacgacc
     1561 gcctggacgc ggctgccgcg catgccgacg caggcgccga ccggatcgat gccgctgtca
     1621 ttggagatga cggcgatctt ggcccggctg ccgggatcgc gcgccacggc gcggatctcg
     1681 atgatggaat catagatctc cggcacttcc tgggcgaaga gctttgccat gaattgcggg
     1741 tgcgtgcgcg acagaaatat ctgcggcccg cgcacctcct ggcgcacgtc atagatatag
     1801 gagcgcacgc gctcgccctg gcgaaagctc tcgcgcggca ggagctcgtc gcgccgcagg
     1861 atcgcctcgg cgcgaccgag atcgaccacc acattgccga attccacgcg cttgaccaga
     1921 ccgttgacga tctcgccgat gcggtccttg tactcgctat attgccgctg gcgttcggcg
     1981 tcgcgcacct tctgcacgat cacctgcttc gcggtctgcg cggcgatgcg gccgaagtcg
     2041 atcggcggca gcgggtcggt gatgaagtcg ccgagctcgg cctcggggtt gaagcgcttt
     2101 gcctcggtga tcgggatctg cgtcgcctcg ttctcgacgg gatcggcaac ctcgcgaaag
     2161 cgcatcaagc ggatctcgcc gttcttgcgg tcaatctcgg cgcggatgtc gtactcctgg
     2221 ccgtatttcg agcggcccgc cttttggatt gcctgctcca tcgcctgcag cacttcgtcg
     2281 cggtcgatgc ccttgtcgcg ggcgacggtg tcggcgactt gcagcagctc ggggcgggca
     2341 tagctggcgc tgggttgcat ggtcatagct ctcggttcag actttctgtt tgcgtacgct
     2401 caatcgcgcg gcggactggt atgggcgagg agctcatccg tcaggacaag cttggctcgg
     2461 gtaacggtgg tgatcggcaa agcgaccggc aaggcatcga cgaggagccg tacatcgtcg
     2521 ccttcgacgc ccaggagccg gccgcggaag cgcttccttc cgtcgaccgg agcgctgagc
     2581 tcgacctttg cctcgaagcc ggcgaagcgc gcgaaatcct cgcggcgcac caaaggccga
     2641 tcgatgccgg gggagctcac ttcgagcaga taagcgccgg cgatcggatc ggccacatcg
     2701 agcagtgccg acaccgtccg gctgatctcg gtgcagtcct cgacgcgcat ggcctgatca
     2761 tcggtccgct cggccatgat ctgcagcgtc gcgcgtcggc cgccggtgac ggcgacgcgc
     2821 accacgcggt aacccatcgc ttcgagcgac ggcgcgatca tcgcctcgat cttggtcgta
     2881 tccacgacac tcctgacttg tgctctgcgc ccgtaaataa aaaagtgggc cttgagccca
     2941 cccttcagcc ggcccgacca ccgatcgggc ctatgggact tttttgagaa atatagtcgt
     3001 tttcgcgcct gctgcaaggc tcaaacgcgg cgcgcctcgc aggccctggg gcggcggcga
     3061 aagcgcagaa acgctggtcg acgcccggca gccagcgctt tccgctcgta gcgcgtcgcc
     3121 ggccagtcct ccggacgctg gcgccaatcg tcgggccgcc gcgccagcca ctcgaaagcc
     3181 ggatgtgccg ctgcgccccc gagcatgacg cgcagatatt ccatgtcatc ggtggcgagc
     3241 cgcagttcgc cgccatcact caagacatgg gccaaggcat cgagcatggc ggcggagacg
     3301 agccgccgct tgtggtggcg ctgcttgggc cagggatcgg gaaagaggat gaagatccgc
     3361 tcgaccgaag ccgcggccag cgattgaacc agcacgcggg cgtcatccac gaaaagccgc
     3421 acattggcga ggcctcgctg cttcacctcg gcgagcagct tgacgatgcc gttctcgaac
     3481 acttcgcagc cgatcaggcc gcggcttggt tgagccgcgg cctgtgccgc caggtgctcg
     3541 cccgccccga agccgagctc gagccatatc gcctccaccg gtgcggcaaa aaggcggcgc
     3601 ggctcgagca gaccttcagc cggcacatcg aagcggagtt gcggcagaag cgatgtgagg
     3661 agttcgcgct gcgcggccct cagcttacgg ccgtggcgac ggccgtagac gacccgcggg
     3721 tgcggcgcat cgatggcggc cgggcctgcg ttcagaacgc cgcccgcaat tctgtgacga
     3781 gatcgagccg ctcccaggag aagccgccat cggcctcggg cgtgcggccg aaatgaccgt
     3841 atgcggctgt gcgggcatag atcggcttgt tgaggcctag atgcgtgcgg atgccgcgcg
     3901 ggctcagatc gacgagatcc tggagcacct gcgagagttt ctcttcgtcg acgcgggctg
     3961 taccggccgt gtcgatatag accgagagcg gccgcgccac gccgatggca taggagacct
     4021 ggatggtgca gcgatcggcg aggccggctg cgaccacatt ttttgccaga taacgcgccg
     4081 cataggcggc cgagcgatcc actttgctcg ggtccttgcc ggagaaagcg ccgccgccat
     4141 gcggtgccgc cccgccatag gtgtcgacaa tgatcttacg gccggtaagg ccgcagtcgc
     4201 cgtccgggcc accgatgacg aaacgccccg tcggattgac gtagaaatgc gcctcgtcgc
     4261 acatccagcc ctgcggcagc acgttcagta catgcggccg cacgatctcg cgcacttcgt
     4321 cctggtcgat gctggccgcg tgctgggtcg agacgaccac tgcggtcgca cgcaccggct
     4381 ggccgtcgat gtattgcagg ctgacctggc tctttgcgtc cggcccgagg aacggcgccg
     4441 ctccggagtg gcgcgcttcg gcgagcgagc gcaagatggc gtgcgcgtag tagatcggtg
     4501 ccggcatgag gctttcggtc tcgcggcagg catagccgaa catgatgccc tgatcgccgg
     4561 cgccctcatc cttgttgccg gcggcatcga cgccttgcgc gatgtcggcc gactgcttgt
     4621 ggagatagca agagacctcg gcgtgcttcc aatggaagcc gtcttgctca tagccgatgt
     4681 cgcggatggc atgacgcgcc acctcgacca gcaggtcttt cgtgatgcta tcgggaccgc
     4741 ggacttcgcc ggccagcacc acccggttgg tggtcgcgag cgtttcgcag gcgacgcgcg
     4801 cctgagggtc ggcggcgagg aaggtatcga cgatggcgtc ggaaatgcgg tcgcatacct
     4861 tgtcgggatg cccctcggag acggattcgc tggcaaaaac atagttgcgc ttggccacgg
     4921 acgatctctc cctgtgctgc cgacgacccg gcgcggccct cgcggcgggt atcaacgctt
     4981 gtccggcaag gagctcccgc cggcaagagc agcgacatgt ggcaaaccgc gcgtcgcgtc
     5041 gtcaagtccc ttccgctgcg cgcgggcggg acgacttcct aggtctcgct ggtgcaggcg
     5101 ttggcgatcg ccttggtcaa gtcgaaaagc cggcgcctga tctgcgcgtc cgggatgcga
     5161 taataagcgc gtaccagctc gagcgtctcg cgtcgcattg tcggatcggg ctcgtaacga
     5221 gcgatcgtcg gctcgatccc gccatcgctt tcctggcgac cggtctcggc cgtctcgggt
     5281 ttcatatcgt cgaagaaaaa cgagacgggt acgtcgagca cgcggcttaa atcataaagc
     5341 cggctggcgc cgatgcgatt ggcaccacgc tcgtatttct gcacttgctg aaatgtgaga
     5401 ccgatcgcct cgccgagttt ctcctgactc atgccaagca aggtgcgccg cagccgcacg
     5461 cgcgagccga catgcacgtc gacgggattg ggtttgtccg atttccggcg gcgtctgcgc
     5521 gtgttcatcc gcgtgttcat tccggcctgt ggcgaaaatt ctttgatcac aggccctatt
     5581 aagtcacacg attgtatgtg gtcaatagtt tgtcgccatt tgatcggtgg ataagtcgca
     5641 aatagtcacg cgcggctgcc gtgacgtccc ccggttaggg tgagggttgc agcgatcgcc
     5701 agcaatgaga gcgagagcgc caaaaatgcc atatcccgcg cgtggtcgta gagcgtcggc
     5761 ggcagggcgc gcggcagttt cgaatcaagc acgccgatcg agtcgagagg gaggcgctgc
     5821 accactctgc catagggatc gacgaccgcg gagatgccgt tgttcgccgc gcggatcaac
     5881 ggcaatccct cttcgacggc tctgacgcgg gtgatggcga gatgctgaaa aggcccggag
     5941 gtgaacccgt accacgcatc gttggtcacg ttgagcagcc attgagggcg ggccgcgtca
     6001 tcgatcactg cgccggggaa gatcgcctcg tagcagatga gcggaccgac gggtggtacg
     6061 ccgggcaagg cgagagtgcg tggcccggcg cccgacgaga aatcgccgat gctcggtacg
     6121 atcttgtcga tcggcacgat gccgcgcaac ggcatgtatt cgccgaacgg cacaagatgc
     6181 gccttgtcat aggtagcgat gatgtcgcct ttgtcgtcga gcacgaccag actgttccag
     6241 acatgcagcg gcggccagcc ctggagcggc tcggcgcgct cggtgcctgt cagcaggagt
     6301 ccacccttcg ggacgaccgc ggcgatcgcc tgccgctctt cggcaaagcg ttcgagcaag
     6361 ggcggcgccg cagcttcggg ccagatgacg tcggtgacct tgtcggtgcc gggcaatgcg
     6421 cttagcgaca agagccgttg aaaattctgc gcccgcgcct gcgggtcgtt cttgaggctc
     6481 tccgggatcg agggctgcac gagacgcaac atcacgcccg gcaccatgtc atgcggcgca
     6541 tgggcgagcc gccacgctcc gcccgccgcc ggtaccgcga tgaggagcag cgcaacgaac
     6601 gcggcgccgc gccggccgct ttcgagatcg ccgagacgtg ccggcaacgc tgcggccgtc
     6661 acggtaagga ggctaagccc atagatgccg acgagcgatg cgacttgcag cacggcgagc
     6721 gcaccgggaa atgtcccggc ccagacgtag ccgacgagat tccatggaaa acccgtcaac
     6781 acatggccac gcagccattc gctgccggcc caggccacgg ccagcaccaa gatgcgtgcg
     6841 gtgccgagga gaccgaagcg gcgcgacacc tcatgcgcgg caagaacagc gagtcccgcg
     6901 aagatggcga ggccggcagg gaagaccaga acggcaaacg gcaagagcca ccagaatgcc
     6961 gatatgtcga cgaacagtgc ggcgccgatc caatagagtc cggcgacgaa gaatccgaaa
     7021 ccgaagctcc agccgagcag cgcggcatcg aaccgcccgg cactgccatc gagcagccac
     7081 acgagaccgc tgaacgagac cgccagtatc ggcggaccgg tcaaacaaat gaccagaaag
     7141 ggcgtgccgt cgagcggcga cagcggcgcg gcggcaagag cgccgagcag ccacgctgcg
     7201 gcatagcggc gccaccccga gagctcagcc gtccacagcg ccgcgcgctg cagcggcgac
     7261 agcggcccgg cggcgcgaaa ggccacgccg gacgtgtcag acatcgcctg gtggcggtgc
     7321 ggcgggcgcc ggcgcaaggc cgcgcacgcg cacgcgcttg atgcgccggg gatccgcgtc
     7381 aagcacttcg aactcgatgc cggccggatg cttgaacacc tcgccgcgtc cgggcacgcg
     7441 cccggcgagc gcgccgacca ggccgcccaa ggtctcgatg ccctcgtcct gttccggcgg
     7501 caaaagcctc gtcggcacac gctcctgaag cgcctggatg cgtgttctgg catcggcgat
     7561 caaagtgccg tccgggcgct cgatcagctt cggttgatcg gcgtcgtcat gctcgtcctc
     7621 gatctcgccg acgatctcct cgatgacgtc ctcgatggtg acgaggccat cgatgccccc
     7681 aaactcgtcg accaccaacg ccatgtgcac gcgcgacagg cgcatctgca acaggaggtc
     7741 gagcaccggc agcgagggtg ccacgaacaa cactttgcgc gcgatcttgc cgagcggcac
     7801 ggtgcgtccg tcggcggcgt aaggcagcag atccttgatg tggacgatgc cgatcacatc
     7861 gtcgagcgtc tcgtgataga gcgggacgcg cgaatggcca aactccacca tctgcttcac
     7921 cgcttcggcg aaaggggtgt caacctcgat ggcggtgatg tcgatgcgcg gtaccatgat
     7981 gtcggccgca ctcagctcgt gcagcttcag gatattgacg agcagtgtcc gctcatgcgg
     8041 atcgatcggc tgctcctcct tatgctcctc gatcagctcc tcaagctcct cgcgcaagct
     8101 cgtttcgccg ttgcgcggcc gagtcagata cttgagccag ttgcgcagca cctggaacgg
     8161 accttcggct tcgcccggcg ggcgattggc ggaagtgtca gccatgcgat cccccgagcg
     8221 ggcgataggg atctggcact cccaattgcg ccaggacggc gatctcgcga gcctccatct
     8281 gcgccgcctc ggcaggggtc gcgtgatcga ggcccagaag atgcagcact ccgtgcacaa
     8341 cgaggtggcg aagatgatcc gcgagcggct taccttgcgc tgccgcctcc gtcgcgatgg
     8401 tctcgaaagc gagcacgacg tcacccagga gccgcggcgc tccttcggga agtgccacat
     8461 cgagcgccgg gaaggacagc acgttggtcg ctctgtcctg ctcacgccat cggcaattca
     8521 aggcgcggag catcgcgtcg tcgcccagca ccaggctgag ctccgccggt ccatggagga
     8581 cctcggcccc ggcaagggcc gcgcgcgccg ccgagaggca gagcgcgtcg acatcgccga
     8641 ggttccgtcg ccacagtggg caggcctcgg cgatatcgat ggcaagcagc gtagcggtga
     8701 cctgcgtctc gccggcgcgg ccggcagagg agggcggccg cgggcggcgc caccgaggac
     8761 gtgcgatacg tttgccagag cgcgggacgc gacgccggcg cagggcagga tcagggccgg
     8821 cggtcatcgc tcttctttga tcgtgaggac gattcgcgct ttgcctcgcg ccggtcgtag
     8881 gcgcgcacga tgcgcgtcac cagcgggtgc cgcactacgt cggagtcggt gaactcgatc
     8941 atggccacgc cctcgacgcc cgcaagcgtg tcgatggcgt cgcgcagtcc ggaatgcgcg
     9001 ccggccggca gatcgatttg cgagagatcg ccggtgaccg ccatccgcgc attctcgccg
     9061 aggcgggtca gaaacatctt catctgcacc ggcgtcgtgt tctgtgcttc gtcgaggatc
     9121 acgaaggcgt tggcgagcgt gcggccccgc atgaaagcga gaggcgcgat ctcgatttcg
     9181 ccgctgctga ggcgcttcat cacctgctct ccggggagca tgtcatagag cgcatcatag
     9241 agcggccgca aataggggtc gaccttctcg cgcaagtcgc ccggcagaaa acccagccgc
     9301 tcccccgcct cgaccgccgg tcgtgacaga atgatccgct cgacgccgcc gctcatcatc
     9361 aagtcgacgg ccgtcgccac cgccagatag gtcttgccgg tgccggcggg gccgaggcca
     9421 aagaccagcg gatgctcgcg catggcgttg atatagctcg cttggtttgc actgcgcggg
     9481 gcgatgcggc gctttctcgt gcggatttcg ttgccttcgc cccaggacgt ggcgccgggc
     9541 tgggctgccc ggtcggcggc gagcctgagc gccgcgtcga ctgccgcggc gtcgacctca
     9601 aggccacgct tcagttggtc gtagagtgcc tcgatcgcgg tgcgtgccac gctggtgcgg
     9661 gcacttggcc ccgatatcgc caggcgattg ccgcgcgaga ccaccgacac gccgagctgg
     9721 cgctcgatgc gatcgagatt ctcgtcacgc tcgccgaaaa gcagcggcaa cagcgtattg
     9781 tcgtcgaact gcatctgcac ccgcccgcct tgggcgccgg attcgccatg ggccgcattc
     9841 atgcggaggc ctccgccaca gcggcggaga gctcgccggc gaggctgttg ggctcggccc
     9901 ggacgaagcg caccgggagc agcgcaccca gcacgtgatc gggtgccgtc gcatgaaccg
     9961 cttgaagata ggggctgcgc ccgacgagct gacccgggtg gcggccacgt ttctcgaaaa
    10021 ggacgggcag ctcgcgaccg accatggcgg cgttgaagcc acgctgctgc gcggcaagca
    10081 actcctgcag ctcggcgaga cgctcggcct tcaccgcctc cggcacctgc tccggcatgc
    10141 ttgccgccgg cgtccccgga cgcgggctgt acttgaagga gaaggcctgc gcgtagccga
    10201 tgtcgcgaac gagagtgagg gtctcggcga aatcggtcgc gctctctccg ggaaaaccga
    10261 cgatgaaatc ggaagagaaa gcgatgtcgg cgcgctccgc gcgcaagcgc tcgatgacgc
    10321 ggcgatagtc gtcggcggtg tggcggcggt tcatggcgcg aagcacgccg tccgaccccg
    10381 actgtaccgg cagatgcagg aacggcatga gctgcggcac ctcgcgatga gcggcgagga
    10441 gcgcgtcgtc gacgtcgagc ggatgcgacg tcgtatagcg cagccgggcg agaccgggaa
    10501 tcgcggcgag cgcgcgaatg agctggccca gtccccagat tccgctatcg ggcgccgcgc
    10561 cctgatacgc gttgacgttc tggccgagca acgtgatctc gcgcacgccg ccggcgacga
    10621 gctgtcgcgc ctcggcgagg agcgcgcttg ccggcctgga gaactcggcg ccgcgcgtgt
    10681 agggcacgac gcagaaggtg cagaacttgt cgcagccttc ctgaaccgag aggaaggcgc
    10741 acacgccgcg cggtatcgtc gtctccggca ggaagtcgaa cttggcttcg gcggggaaat
    10801 cggtgtcgag aacctgaacg cccgaccgca tcacctgcgc caccatctgt ggaagacgat
    10861 ggtaagtctg cggcccgaaa acgatgtcga catagggagc gcgcgcgagg atttccgcgc
    10921 cctccgcctg cgccacgcag ccggcgacgg cgagaatcag gcggttgctc gaagcgccct
    10981 tgcctgcctt cagcggcctg agccggccca gctccgagaa caccttctct gacgccttct
    11041 cgcggatgtg gcaggtattg aggatgatca tgtcggcctc ttccgcggcg tctaccagcg
    11101 cgtagcccaa gggcgcgagc gcatcggcca tgcgcgcgga atcataggcg ttcatctgac
    11161 agccataagt tttgatgtag agcttcttcg ccaagatccc tctgcctcct gttcgcgtca
    11221 ctcggcgccg atggcttcct gcgccggcgt cggctgcggg gcttcgacag gcggcgacgc
    11281 cggttcatcc gttcgccccg ccagcgcccg cgcaacccct tgcgcgatca cgcgatggca
    11341 atgctcggcg agcgctttgc gcgacccgaa ttgcgcaagc gtgaccgccc gatgaaaggt
    11401 cacatccact tcgaccgtgc cgaggccgag catggcccaa agatggggcg ccagctccat
    11461 atcgccgtac caggcgaaga acgggcgata aaagcgcccg agcgggatac cgtcgagccg
    11521 cgtataggcg agcgacaccg gctggacggc caaggcaccg caggctcctt tgtagtcggc
    11581 cacgctgaag agcgcactct tgaacggcaa cacgcggttg ccgtcattgg cggtgccctc
    11641 gggaaagagg atgagattgt cgccggcgtc caggcgctcg cggatggcgt cgcgctgcat
    11701 tgcagtgctg cgcacgcgac gatcgacgaa caccgtgcgc tgcaacttgg cgagccagcc
    11761 gaaaaacggc cagcgcgcga cctcatgctt ggcgacgaac gagccttcga tcagcgctcc
    11821 gaggatctcg atgtcgaggt aagaggtatg attcgcgatg aacaaggtcg gccgttccgc
    11881 cgagcgctct cctccggtct tgatgtggaa tccgagaatg cgcgcgctcc agcggtgata
    11941 aattcgcggc aaccgccgcg cgagcggtcg cttcagcaaa agtgccgcgg cctggaccgg
    12001 cataagcggc agcgtcagcc ccagatagag ggacaatcga ccgaggcgga gcagcgtggc
    12061 actcatggct cacgcggcct gtccgtcccg cgcctggcgc tcgtagtgcc gatagtactt
    12121 atcggtgacc agatcggtct tcacgaccac gcagacatcg gtggtgttga actgatgatc
    12181 gatgacggca ccatcgccga cgaagccgcc gagccgcaga tatcccttga tgagcggcgg
    12241 caggtcgcca agcgcgcggg ctggatcgag gacgtcggcg gggagacgcc gcatctcgac
    12301 ccgacgctcg ggcagtgccg acggcctcaa gccctgcggt gccagatggt ggtgatagag
    12361 ataggaaagc gaggtcgcga gcgcatcgac atcggtgccg ggcaggctcg cgcagccgaa
    12421 catgagggcg atgtcgtatt ggaagacgta ggcggcgatg ccccgccaca gcagctgcat
    12481 ggtcggccgg ttgcgcgccg acgcgtcgac gcaggagcgg ccgagctcga ggatctcgcc
    12541 gggatacgca agcatctccg cgatatcgta ctcggccgcg gaatagaagc tgccatggtg
    12601 ggccgccgcc gagcggcgga tcaggcgata agtcccgacg accgcggcgg ctccgctgcc
    12661 gcgcgcatga tcgaccacga ggagatggtc gcagacgccg tcgaagctgt cgaagtcgcg
    12721 ctcgatgcgc gccatctcgg cacttgggct cgcccccatc tcgtcataga agacccggta
    12781 gcgcagggcc tgcgccgcat cgatgtcagc catcgtctcg gcgagccgga cctggagcgc
    12841 gcccgagcgg acatcgacga tcgacagcgc ttcggtcggc tgggcgcgct gcggctcgat
    12901 cacggcgccc cgcggcggca agcggtcatt tgcgctcgac cttgccggcc agcggcacgg
    12961 cgaagagttc gaggcggtga tcgaccagtc ggtagcccag ccgctcggcg acgtcgcgct
    13021 gcagtttttc gatatcctcg ttgcggaact cgatgacgcg gccggtct
//
LOCUS       DASYNS010000438         4858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2608145, whole genome shotgun sequence.
ACCESSION   DASYNS010000438 DASYNS010000000
VERSION     DASYNS010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2608145"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2081
                     /locus_tag="VGP50_13580"
     CDS             <1..2081
                     /locus_tag="VGP50_13580"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV8028459.1"
                     /translation="FAARATAAGSWRTVALDLSYRSTPAVLQAVDAVFARAEARDGVA
                     LDNLPIRHRPVREDHAGLVELWPAVEPEEAPAMRPWEPPLAQRRRREPRARLAFAIAA
                     TVRSWLETGERLPARDRRVEPGDVMVLVRRRGSFVIELVRALKEVDVPVAGVDRILLT
                     DQLAVQDMIALGQFLLLPEDDLTLATVLKGPLFGFDEEQLFALAHPRMGTLWHELRQR
                     AAEHPVFGQAAQRLQELLARADFTPPYELFAEVLGAGGGRRAALERLGPEAGDPLDEF
                     LSMALAYEAMHAPSLQGFLHWLGRGETEVKRDLDQRGRNEVRVLTVHGAKGLQAPIVF
                     LPDTLQVPTQPLRLLWSDTGLPLWSAHRGCGAPALDRALAEARRRREAEHRRLLYVAM
                     TRAEDRLYVCGWRTKVRAPEGCWYHLVEQGLAGAPGAEAITQDLSRVAGGDGWAGPGW
                     RLESRQLLVPRAAEILPLPLERVLPLPDWAKRPPAPEPSPPRPLVPSQPRRADPAARS
                     PLGAEEGLGLVRGRLVHRLLQSLPDVPPARRGAAARRFLALPVHGLAPEAREALLAET
                     LAILAHPDFAPLFAPGSVAEVPVIGLLGELALAGQIDRLVVGEAAVLIVDYKTLRPPP
                     LDEADVPEAYLDQLAAYRAAIATIYPDRPVRCALLWTEGPRLMPISAAALDRRDPAIR
                     SRGAHAVSGT"
     gene            2435..2755
                     /gene="trxA"
                     /locus_tag="VGP50_13585"
     CDS             2435..2755
                     /gene="trxA"
                     /locus_tag="VGP50_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEV8028460.1"
                     /translation="MNTSKVTDSSFDADVLRAPGPVLVDFWAEWCGPCKAIAPHLEEL
                     ATEMKGKVTVAKINIDENPQTPMKYGVRGIPTLILFKNGQVAATKIGQLPKSKLLEWV
                     ESVL"
     gene            complement(2773..3144)
                     /locus_tag="VGP50_13590"
     CDS             complement(2773..3144)
                     /locus_tag="VGP50_13590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028461.1"
                     /translation="MSKRILIIDDDLAVRDTLIAILEDRGYEVMTAVNGQQGLAVFRS
                     ERPDLLVTDIVMPVKEGLQTIREIRAERPDMKIIAISGGSRTERHDFLEIARQLGAWD
                     VVVKPFDAEDFVARVDRCFAA"
     gene            complement(3141..4616)
                     /locus_tag="VGP50_13595"
     CDS             complement(3141..4616)
                     /locus_tag="VGP50_13595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028462.1"
                     /translation="MPIPVAQIPTPAVVLDGKGLVYAANDAMAGCLGVARQELIGTEF
                     AGWADDRDAMRDFLRRAGGRQQEFDFRTRNGSRRAIAISIGSSDARNGGLLLGFDTTA
                     LRECERRYCAMFEVASDWFWETDGALRLSHLSPNFETAMGMPGRALLGKRRDEVADTT
                     EDADRWRVHLAMLAARRPFRDFVCRQAGSDGRRRWLKSSGVPMFDAEGGFTGYQGATT
                     DVTAYLEAEAAADHKRRSLEAELQHAQKLEALGALAGGIAHDLNNTLVPVIALAKITV
                     DKLPEHSRERRNLATILEAGARARDLVRQILAFTRKEAPTRTTVELSGLVRSSLKLLR
                     ASIPSTIHIDEAIAEVPPVRGDPGQIHQIMINLVVNAAQAIGEAPGKIAIELATAPSD
                     RLDQAQHPWIGPAVRLSVQDSGCGMDEATIKHIFEPFFTTKAVGDGTGLGLSVVQGIV
                     AQHGGRIAVESRRGQGTRFDVYLPALSREEARQLDMSGAAA"
BASE COUNT          719 a         1653 c         1665 g          821 t
ORIGIN      
        1 atttcgcggc gcgcgcgacg gcagccggaa gctggcgcac cgtcgccctc gatctctcct
       61 accgctccac gccagccgtg ctgcaggccg tcgatgccgt cttcgcccgc gccgaggcgc
      121 gggacggcgt cgccctcgac aacctgccga tccgccatcg ccctgttcgc gaggatcacg
      181 ccgggctggt cgagctctgg cccgccgtcg agccggagga ggcgccggcg atgcgtccct
      241 gggagccgcc gctcgcccag cgccgccggc gcgagccgcg ggcgcggctg gccttcgcca
      301 tcgcggcgac ggtgcgaagc tggctcgaga cgggtgagcg cctccccgca cgcgatcggc
      361 gcgtcgagcc gggcgacgtc atggtcctgg tgcgccggcg cggctctttc gtcatcgagc
      421 tggtgcgggc gttgaaggag gtcgatgtcc ccgtggccgg ggtggatcgc atcctcctca
      481 ccgatcagct cgcggtgcag gacatgatcg cgctgggcca gttcctgctg ctgcccgagg
      541 acgacctcac gctcgcgact gtgctcaagg gccctctctt cggcttcgac gaggagcagc
      601 tcttcgcgtt ggcgcatccg cgcatgggca cgttgtggca tgagctgcgc cagagggccg
      661 ccgagcatcc ggtcttcggc caggccgcgc agcgcctcca ggaactcctt gcccgcgccg
      721 atttcacacc gccctacgag cttttcgccg aagtgctggg cgcgggcggc ggccgccgcg
      781 ccgcgctcga gaggctgggg cccgaggccg gcgatccgct cgacgagttc ctatcgatgg
      841 cgctggccta tgaagccatg catgcgcctt cgcttcaggg ctttctccat tggctcggcc
      901 ggggcgagac ggaggtcaag cgcgacctcg accagcgcgg ccgcaacgag gtgcgcgtgc
      961 tcaccgttca tggcgccaag ggactgcagg cgcccatcgt gttcctgccg gacacgctcc
     1021 aggtgccgac ccagccgctg cgcctgctct ggagcgacac cggcctgccg ctatggagcg
     1081 cgcatcgcgg ttgcggcgct ccggcactcg accgcgcgct cgccgaggcg cggcggcggc
     1141 gcgaggccga gcatcgccgc ctcctctacg tggcgatgac ccgtgccgag gaccggctct
     1201 atgtgtgcgg ctggcgcacc aaggtcaggg cgcccgaggg ctgctggtac catctggtcg
     1261 agcaggggct cgccggcgcg cccggtgccg aggcgattac gcaggatctg agccgcgtcg
     1321 ccggcggcga tggctgggcc gggccgggct ggcgcctcga atcccgccag cttctcgtgc
     1381 cgcgcgccgc cgagatcctg ccgctgccgc tcgagcgggt tctgccgctc cccgactggg
     1441 ccaagcgtcc gccggcgccc gagcctagcc cgcctcggcc gctggtcccg tcgcagccgc
     1501 gccgggccga cccggccgca cgctcgccgc tcggcgccga ggaggggctg ggcctcgtcc
     1561 gcggccgtct cgtccatcgg ctgttgcaaa gtctcccgga cgttccaccg gcccggcgcg
     1621 gcgcggccgc ccgacgcttc ctggcgcttc ccgttcatgg cctcgcgccg gaggcgcggg
     1681 aggcgctgct ggccgagact ctggcgatcc tcgcgcatcc cgatttcgct ccgctcttcg
     1741 caccgggctc ggttgccgaa gtgccggtga tcgggctctt gggcgagctg gcgctggccg
     1801 ggcagatcga ccggctggtg gtcggcgagg ctgccgtgtt gatcgtcgac tacaagaccc
     1861 tccgcccgcc gccgctcgat gaggcggacg tgcccgaggc ctatctcgac cagctcgcgg
     1921 cttatcgcgc cgccattgcc acgatctatc ctgaccgccc ggtgcgttgc gcactgctct
     1981 ggaccgaagg cccgcggctc atgccgatca gcgcggccgc gctcgaccgc cgtgatccgg
     2041 ccatcaggag tcgcggagcg catgcggtca gcggcacctg attcccgatc cgggggcctc
     2101 tgacagcctc gagcgtcagg aagacttcgc tgaacctcgg ttgagccgag cccggtaagc
     2161 gtctcgcgcc ggcggcatta cgggcacaga gagctgtcgt cggcggcgat tgaaggtgcg
     2221 cgccgactca tcggcgatgc cgaagcgggt cgagaaaaga gcagcggcgg cgcgaagtcg
     2281 cacaaacgag tccgatccaa agccgtgaag cgcaagaaat cgcggtgaat gcgattgaat
     2341 ggcccgtctg tcggacaaac gggtcggacc ggcccgtctt gacgctgctt ttgcgcatac
     2401 ctaatctctt gtcgtcctgg ttcggaggaa gcgcatgaac acaagcaagg ttaccgattc
     2461 gtccttcgat gcggacgtgc tgcgggctcc tggtcctgtg cttgtcgatt tctgggccga
     2521 gtggtgcgga ccttgcaagg caatcgcacc acatctcgaa gagctggcga ccgagatgaa
     2581 gggcaaggtg acggtcgcca agataaacat cgacgaaaat ccgcagacgc cgatgaaata
     2641 cggcgtccgc ggcatcccga ccttgattct gttcaagaac ggccaggtcg ccgcgacgaa
     2701 gatcggtcag ctgccgaaga gcaagctgct cgagtgggtg gaatcggtcc tctgacgggg
     2761 ggcgcgcggg cgtcaggcgg cgaagcagcg gtcgacccgg gcgacgaaat cctcggcatc
     2821 gaatggcttg acgacgacgt cccaggcgcc gagctgacgc gcgatctcca ggaagtcgtg
     2881 ccgctcggtg cggctgccgc cggaaatggc aatgattttc atgtccggcc gctctgcgcg
     2941 aatctcgcgg atcgtctgta gtccttcctt cacgggcatg acgatgtcgg tgacgaggag
     3001 gtccggccgc tcgctgcgga agaccgcgag tccctgctgc ccgttgaccg cggtcatgac
     3061 ctcatagccg cgatcctcga gaatggcgat cagcgtgtcg cggacggcga gatcgtcgtc
     3121 gatgatgagg atgcgcttgc tcaagctgca gcaccgctca tgtcaagctg acgcgcctct
     3181 tcccgggaga gcgccggcag gtagacatcg aaccgcgtgc cctggcctct ccggctctcg
     3241 accgcgatgc ggccgccatg ttgggcgacg atcccctgta ccaccgacaa accgaggccg
     3301 gtgccgtcgc ccacagcctt cgtcgtgaag aacggctcga agatatgctt gatcgtcgct
     3361 tcgtccatgc cgcagccgct gtcctgcacg gaaagccgca cggccggccc gatccatgga
     3421 tgctgcgcct ggtcgagacg gtcgctcggc gccgtcgcga gctcgatggc gatcttgccg
     3481 ggcgcctcgc cgatcgcttg cgcggcgttg acgactaggt tgatcatgat ctgatggatc
     3541 tgcccggggt cgccgcgcac cggcggcacc tcggcgatcg cttcgtcgat atggatggtc
     3601 gagggaatgc tggcgcggag cagcttcaga gagctgcgga cgagcccgga gagctcgacc
     3661 gtcgttctcg tcggcgcctc cttgcgcgtg aaggccagga tctgacggac gagatcgcgc
     3721 gcccgcgccc cggcttctag gatcgtcgcc aggttgcgtc gctcgcggct gtgctcgggc
     3781 agcttgtcga ccgtgatttt tgccagcgcg atcaccggga cgagcgtgtt gttgaggtcg
     3841 tgcgcgatgc cgccggcgag cgcgcccaag gcttcgagct tctgcgcgtg ctgcagctcg
     3901 gcctcgaggc tgcggcgctt gtggtcggcc gcggcttccg cttcgagata cgcggtgacg
     3961 tcggtcgtcg cgccctgata tccggtgaag ccgccttcgg catcgaacat cggaacgccg
     4021 ctcgacttca gccagcgtcg ccggccgtca ctgcccgcct ggcgacagac gaaatcgcga
     4081 aagggtcgtc gcgcggccag catcgcgaga tggacgcgcc agcggtcagc gtcctcggtc
     4141 gtgtcggcga cctcgtcgcg gcgcttgcca agcagcgccc gtcccggcat gcccatcgcg
     4201 gtctcgaaat tgggcgatag atggctcagc cgaagcgctc cgtcggtctc ccagaaccag
     4261 tcggaagcga cttcgaacat ggcgcagtag cgccgctcgc attcgcgcag cgccgtcgtg
     4321 tcgaagccga gtagcagacc gccattccgc gcgtcgctcg acccgatcga gatggcgatc
     4381 gcgcgccgac tgccgttgcg ggtgcgaaag tcgaactctt gctggcggcc accggcacgg
     4441 cgcagaaaat cgcgcatggc gtctcgatca tcggcccagc cggcgaactc cgtgccgatc
     4501 agctcctgcc gcgccacgcc caggcaacca gccatggcgt cattcgccgc atagacaaga
     4561 cccttgccat cgagcaccac cgctggggtc ggtatctgcg cgacggggat gggcatgggg
     4621 attttctatc gaccggcgtc gtgggcaagg caggcgcagc aactttagag ggcaatgcta
     4681 gcccggattc gtttacgaac ggttctggcg cgctatgcgc ttcatacagc ggcatccacc
     4741 cgagaagcag gcggtttcca gccgagacag ggcgcgcttg agctttgccg agagggcatc
     4801 gattcggggt cttatgggag cgctaactat ttctcaattt gagagtatta gcgttccc
//
LOCUS       DASYNS010000439        11691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3544682, whole genome shotgun sequence.
ACCESSION   DASYNS010000439 DASYNS010000000
VERSION     DASYNS010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3544682"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VGP50_13600"
     CDS             complement(<1..507)
                     /locus_tag="VGP50_13600"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8028463.1"
                     /translation="MARKAWRPSSRSARPNSRTASAAARKASQIAMHPVDVVVRRAEE
                     KDAVSILDMARELAAAVEDPAPRIDVARLVGDLLDDARWGECFIAERGGAPAAYALAC
                     RSFEIHTRQRRLWLCDLYVRPEARRSGIGRTLIAAIARHALSLGCTAVYWELWRKNRV
                     GESFYREVG"
     gene            complement(446..1222)
                     /locus_tag="VGP50_13605"
     CDS             complement(446..1222)
                     /locus_tag="VGP50_13605"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV8028464.1"
                     /translation="MAYECILVERHGKVGIVRLNRPKALNALCAALVRELGQALDAFE
                     ADDAIGCIVLTGSEKAFAAGADIKEMQAMDFPDTYLADFITLGWERVTTCRKPIVAAV
                     AGYALGGGCEMAMMCDTIIAGDTAKFGQPEITLGTIPGAGGTQRLTRFVGKAKAMDMC
                     LTGRMMDAAEAERSGLVSRVVAPDRLMAEALAVAEKIAAMSRPSAMMAKECVNRAFET
                     TLAEGVRFERRVFHATFALADRKEGMAAFIEKRPPQFKNR"
     gene            1393..3138
                     /locus_tag="VGP50_13610"
     CDS             1393..3138
                     /locus_tag="VGP50_13610"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8028465.1"
                     /translation="MGSELGRETDEATPAAAVDTDALMRQAMAFHRAGLLDKAALLYR
                     QIVEHDPAHRDALHFFGVLSHQQGDDTAAVALISRAIAIDDSDPAFHGNLGIALKGCG
                     RLDAAVAAYRRAIALAPEFPEAHNNLGIALYGLGKVEAAAAEYREALRHRPNYADAHF
                     NLAVALRSAKALEDAVGAYQDALRCRPKHAEAMHGLAAALRELGRSDAAVAPYARYIV
                     ERPDDVEAIKALGDLLISLKRGDEALAVYAAGLERRPNDPELLNNIGVALMGQGRMDE
                     AAAVFAAALEHKPDFADAQYNAGVVFERSGRTEEAIAAFEETLKLRPAHPQAHHALGA
                     LYTVLGRRDDGARIYRHWQSVAPDHPVARHMAAAVDRTETPTRAADAYIRNLFAPFAL
                     TFDQVLAKLNYRVPELMAAVLARFPSSHLLDVADIGCGTGLCAAFLRPLARRLVGVDL
                     SPEMLDKARDRQLYDLLVEAELTAFLNGNPAAFDLVVIADTLVYFGDLAPAFAAVGGA
                     LRSGGRVMFNLERDAAPPADGYRLNEYGRFVHGEDHVRRRIAEAGFTVLALDVAAIRE
                     QAKEAVPGFVVLAAK"
     gene            3403..4443
                     /gene="tssA"
                     /locus_tag="VGP50_13615"
     CDS             3403..4443
                     /gene="tssA"
                     /locus_tag="VGP50_13615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEV8028466.1"
                     /translation="MATIELERLLEDISPDAPCGGNLEYDSDFVLLEQSARGKPEQQF
                     GNTIIPAEEPDWAAVRRIALDLSKRTRDLRVMDYLARASIRTHGYAGLNDVLTLLRRA
                     LERYWEPIHPQLDPDDDNDPTARVNIIGSLCDAQTMLAGIRMAPLVQSRALGRFGLRD
                     ISIASGETQPPAAMDAPPTMQAIEGAFLDSAIEELQATADAVRSALEQTRAIEGVVTE
                     KVGTGNAASLDPLSAVLKDASRAIETRLAARGVGTVATATEVVAVNGHATNGSAASFA
                     VAGQLQSRDEVMRLLDKICEYYARYEPSSPVPLLLQRAKKLVPMDFMEIMKDLIPDAV
                     ATAEMYRGRLPE"
     gene            4523..5050
                     /gene="tssB"
                     /locus_tag="VGP50_13620"
     CDS             4523..5050
                     /gene="tssB"
                     /locus_tag="VGP50_13620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEV8028467.1"
                     /translation="MAKMSSQKFIARNRAPRVQIEYDVEVFGSQKKVQLPFVMGVFAD
                     LSGKPAEALPAVADRKAVLIDVDNFDDRLKSMKPRVAFQVPNTLTGEGNLNVELTFES
                     MDDFSPAAVARKVGALNQLLEARSQLANLVAYMDGKSGAEELIGKMLTDPTLLKAIAA
                     APKPQDAQPEAGAQE"
     gene            5057..6547
                     /gene="tssC"
                     /locus_tag="VGP50_13625"
     CDS             5057..6547
                     /gene="tssC"
                     /locus_tag="VGP50_13625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEV8028468.1"
                     /translation="MAETQEIGQTQAGTAEFSDFGALLNKEFRPKSEHAQEAIQNAVK
                     TLAAQALESSVVVSNDVVNTINAIIAELDRKLTEQINLIIHHADYQKLEGAWRGLSYL
                     VNNTETDEMLKIRVLNISKQDLGKTLRKFKGTAWDQSPIFKKMYEEEYGQFGGEPYGC
                     LVGDYYFDQSPPDVELLNNIAQVSAAAHCPFIGAAAPSLMQMDSWQELSNPRDLAKIF
                     STPEYAAWKSLRDSEDSRYIGLAMPRFLARLPYGAKTDPVEGFDFEEVTEGGKHDKYC
                     WANSAYAMAVNINRSFKLYGWCSRIRGIESGGAVEGLPAHTFPTDDGGVDLKCPTEVA
                     ISDRREAELAKVGLMPLIHKKNTDFAAFIGAQSLQKPAEYEDPDATSNANLSARLPYL
                     FATCRFAHYLKCIVRDKIGSFKERPDMQRWLQEWIMNYVDGDPANSSEETKARRPLAA
                     AEVVVEEVEGNPGYYSSKFYLRPHYQLEGLTVSLRLVSKLPSAKAA"
     gene            6592..7077
                     /locus_tag="VGP50_13630"
     CDS             6592..7077
                     /locus_tag="VGP50_13630"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEV8028469.1"
                     /translation="MAMDIFIKIGDLKGESVDDKHKDEVQVLAWSWGMSQSGTTHSGT
                     GSGGGKGSFQDLSFTHYIDKVSPNLMLACANGKHFSEALLTVRKAGEKPLEYLKITMT
                     DLIVTSVTTGGSGGEDRLTENVSLNFSKFKVEYTPQKKDGSGDAPITIGWDIAGNVKT
                     G"
     gene            7147..7983
                     /locus_tag="VGP50_13635"
     CDS             7147..7983
                     /locus_tag="VGP50_13635"
                     /inference="COORDINATES: protein motif:HMM:NF018699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HEV8028470.1"
                     /translation="MGMEDWLRGGDPSTALSDVQEQVRKNPANAKLRIFLFQLLAIEG
                     RWDRALTQLKVAGDLDAGSLAMVQTYREALACEALRDEIFAGTRSPLVFGQPENWIAL
                     LIEALRLAAVGKVEQSQQIRAEAFDAAPTTRGTIGDQAFEWIADADQRLGPVLEAIIN
                     GRYYWVPFHRIRAVRFDAPADLRDVVWMPAYFTWANGGESVGLIPTRYPGSQSSDNPH
                     LRLSRRTEWIERGADLFIGMGQRMLATDAGEHAIMDLRAIKLDTAEESADPGTAQPAA
                     MA"
     gene            7996..8514
                     /gene="tssE"
                     /locus_tag="VGP50_13640"
     CDS             7996..8514
                     /gene="tssE"
                     /locus_tag="VGP50_13640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HEV8028471.1"
                     /translation="MAELTQQERLQPCLLDRLTDDEPEKKQEARDQRVVSLRRLREGV
                     LRDLGWLLNATNLAVLEDLADYPDVAKSVLNYGMPALAGTLVSGIDVNDFERKIYQAI
                     VDFEPRILRDTLKVRLAISGDEMSHNAMTFEIEGQLWAQPVPQRLYLKTEIDLELGEV
                     KLSDYSGGASSG"
     gene            8514..10391
                     /gene="tssF"
                     /locus_tag="VGP50_13645"
     CDS             8514..10391
                     /gene="tssF"
                     /locus_tag="VGP50_13645"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEV8028472.1"
                     /translation="MDPRLLKYYNAELQHFRGMSGEFAAEFPKVAGRLGLDSFECADP
                     YVERLIEGFAFLAARVRLKVDAEFPRFTRHLMQMVYPHYLSPTPSMAVVQFRPLPDEA
                     ALAEGSVVPRETVMKTLLGRGEQTPCEYRTAHDVTLWPLELVDAEYIGSTAAVIALGV
                     TEVQGVRAAIRIKLKTTGVLTFDKLALDSLPLFLRGSNELPVQIYEQLIANVAGMVVR
                     PATRPIAWQHVVRDEPTRPLGFDAKEALLPYQAPSFDGYRLLHEYFAFPQRYLFVELT
                     GLRPAVRRCTTNELEIIILLKRQAPRLDGAISKEEFALFCSPAINLFPRRADRIHLTD
                     RSDEHHVVVDRTRPMDFEVYAVTGVTGFGVGNENQQEFLPFYALTDLAAMAEARAFFA
                     TNREPRLLSSRQRARGPRSSYIGSELFISLVDGNEAPYRHDLRQLGVETLCTNRDLPL
                     DVPIGKGPTDFILQLSAPVESVRCVAGPSKPRPSFGEGDAAWRLISHLSLNYLSLVDS
                     NPEQGAAALRELLSLYADLDELHITRQLEGLRSISTRPVTRRALIPGPISFCRGLEIT
                     VTCGEESFEGTGVFLLGAVLDRFFAKYVSINSFTETVLRTLGRGEIMRWRTRIGQRHT
                     L"
     gene            10355..11386
                     /gene="tssG"
                     /locus_tag="VGP50_13650"
     CDS             10355..11386
                     /gene="tssG"
                     /locus_tag="VGP50_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEV8028473.1"
                     /translation="MADADRTAAHALALLRNLQEKPYEFGFFQAVRRLENLYRDKPRT
                     GTSVHASDDPIRLAQEPSTAFAPSTLARFTPGEGNARPRLSGYFLGLLGPNGPLPIHL
                     TEYARERMRNLGDATFTRFLDLFHHRMLSLFYRAWAINEPAVSFDRPEDDRFAIYVAS
                     LFGMGSPALRNRDAFPDLARLRYAGLFANQTRHGSGLCAVLRDFFRMPVAIREFTAGW
                     LHLGEDMWWRLGHSRASGTLGQSATIGARVWSCHHKFRIAFGPLTLTQYLRLLPGGES
                     LRRLVPLVRSYVGDELDWDVNLILKREEIPPLELGRQGRLGWTTWLTAPRRTGDAGDL
                     RLNPLAAAR"
     gene            11454..>11691
                     /locus_tag="VGP50_13655"
     CDS             11454..>11691
                     /locus_tag="VGP50_13655"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEV8028474.1"
                     /translation="MTEISRAALFGKLNSLGYRAIESATVFCKMRGNPYVELVHWLHQ
                     ILQQQDSDIHRIIKYFGLDAGRLAKDVVDALDRLP"
BASE COUNT         2056 a         3926 c         3725 g         1984 t
ORIGIN      
        1 gccgacctcc cggtagaagc tctcgccgac ccggttcttg cgccacagct cccaatagac
       61 cgccgtgcag ccgaggctca gcgcgtgccg cgcgatcgcc gcgatgagcg tccgcccaat
      121 accgctgcgc cgcgcttccg gtcgcacata gagatcgcag agccatagcc gccgctgtct
      181 ggtgtggatc tcgaagctgc ggcacgcgag cgcgtaggcc gccggcgctc cgccgcgctc
      241 cgcgatgaaa cactcgcccc agcgcgcgtc gtccagcaga tcgccgacca gccgggcgac
      301 gtcgatgcgc ggtgccgggt cttcgacggc cgcggccagc tcccgcgcca tgtcgaggat
      361 cgataccgcg tccttttctt cggcacggcg aacaacgaca tccacgggat gcatcgcgat
      421 ttggctggcc tttcgcgcag ccgcgctagc ggttcttgaa ttggggcggg cgcttctcga
      481 tgaaggccgc catgccttcc ttgcgatccg cgagcgcgaa ggtggcgtgg aaaacgcggc
      541 gctcgaatct cacgccttcg gccaaggtcg tctcgaaagc gcggttcacg cactctttcg
      601 ccatcatcgc cgacggccgc gacatcgcgg cgatcttctc ggcgacggcc agcgcttccg
      661 ccatcagccg atccggcgcc acgacgcggc tcacgaggcc ggaacgctcc gcctcggccg
      721 catccatcat gcgtccggtg agacacatat ccatggcttt ggccttgccg acgaagcgtg
      781 tgaggcgttg cgtgccgccc gctcccggaa tcgtgccgag cgtgatctcg ggctgaccga
      841 acttcgccgt gtcgcccgca atgatcgtgt cgcacatcat cgccatctcg cagccgccgc
      901 cgagcgcata gccggccacc gcagcaacga tcggcttgcg gcaggtcgtg acccgctccc
      961 accccagcgt gatgaaatcg gccaggtagg tatcgggaaa gtccatcgcc tgcatctcct
     1021 tgatgtcggc gccggcggcg aaggcctttt cgctgccggt aaggacgatg cagccgatcg
     1081 cgtcgtccgc ctcgaaagcg tcgagcgcct ggccgagctc acggaccagc gccgcgcaaa
     1141 gggcgttgag cgctttcggc cgattgagcc ggacgatgcc gaccttgccg tggcgctcga
     1201 cgaggatgca ctcatatgcc atcggaacag cctctcggtt tgaccaagga tcggcgcgag
     1261 cgcgccgggg cggcgagaga tatagcactg cgccgttcgc gcccgccatg accgccgctc
     1321 cgcctttgtg gtagacatgg gcatgcgcgt aagcccgcag cgctgcggcg ggctggtcgc
     1381 cagaggtgct ccatgggctc cgaactcggt cgtgagaccg atgaggctac gccggccgct
     1441 gccgtcgata ccgatgccct gatgcgtcag gcgatggcat tccaccgcgc cggccttctc
     1501 gacaaggcgg ctttgctcta ccgccagatc gtcgagcatg acccggcgca tcgcgacgcc
     1561 ctgcattttt tcggggtgct gtcgcatcag cagggcgacg acactgccgc ggtggccctg
     1621 atcagtcggg ccatcgccat cgacgacagc gatcccgcct ttcacggcaa tctcggcatc
     1681 gcgctcaagg gctgcggccg gctcgatgcc gccgtcgccg cttatcgccg cgccatcgcg
     1741 ctggcgcccg aattccccga agcgcacaac aacctgggca tcgcgctcta cggcctgggc
     1801 aaggtcgagg cggcggccgc cgagtatcgc gaagccttgc gccatcgccc gaactacgcc
     1861 gatgcgcatt tcaatctggc ggtcgcgctg cgctcggcga aagcgctcga agacgcggtc
     1921 ggggcctatc aagacgcctt gcgctgtcgc ccgaagcatg ccgaggcgat gcatgggctt
     1981 gccgcggcgc tgcgcgagct gggccggtcc gatgcggcgg ttgcgcctta tgcgcgctac
     2041 attgtcgagc gccccgacga cgtcgaggcc atcaaggctc tgggggatct gctgatctcc
     2101 ctgaaacggg gtgatgaggc gcttgctgtc tatgccgcgg gcctcgaacg gcggcccaac
     2161 gatcccgagt tgctcaacaa tatcggcgtt gccctcatgg gccaggggcg catggacgaa
     2221 gccgccgcgg tgttcgcggc agccctcgag cacaagccgg acttcgccga cgcgcaatat
     2281 aacgccggcg tcgtcttcga gaggagcggc cggaccgagg aggccatcgc ggccttcgag
     2341 gagacactga agctgcggcc tgcccacccc caggcgcatc atgccctggg tgcgctttat
     2401 acggtgcttg gccgccggga tgacggggcg cgcatctatc ggcactggca aagcgtggcg
     2461 cccgaccacc cggtggcgcg ccacatggcc gcggccgtcg accggaccga gaccccgacc
     2521 cgcgccgcgg acgcttacat ccgcaacctc tttgcgccct tcgccctgac cttcgaccag
     2581 gtcctggcga agctcaatta tcgcgtaccg gagctgatgg cggcggttct ggcccgcttc
     2641 cccagcagcc atctgctcga cgtcgcggat atcggctgcg gcaccggatt gtgcgccgcg
     2701 ttcctccgac ccctggcgcg ccggctggtc ggcgtcgatc tctcgcccga gatgctcgac
     2761 aaggcgcgcg accgccagct ttacgacctc ctcgtggagg ccgagctgac ggcgttcctc
     2821 aacggcaacc cggcggcctt tgatctcgtc gtcattgccg acaccttggt ttatttcggc
     2881 gatctcgcgc cggcgtttgc cgccgtcggc ggcgcgctgc gctccggcgg ccgcgtcatg
     2941 ttcaatctcg aacgcgatgc cgcgccgccg gcggatggtt accgcctgaa cgaatatgga
     3001 cgttttgtcc atggcgaaga ccatgttcgc cggcgcatcg ccgaagccgg gttcacggtc
     3061 ctggcgctcg acgtcgcggc gatccgcgag caggcgaaag aggcggtgcc gggcttcgtt
     3121 gttctggcgg caaaatagcg cagctccgga caggcggcga tatagcgggc cacgggtgct
     3181 caaaatggcg attaactttt tcagagtgtg aagtccgtca catctgttac gcgacataat
     3241 tgcaatattg gcgccaacga cgagccgaat tgtttgactc tgtcggacgc gacatctacc
     3301 ttcgggcgtt gagcattacc cataagattg aaggccgccg agacagagaa gcggccgctc
     3361 tctccaggga ggagcgcgcc ttttcgggca cgcgagatat ttgtggcaac catcgaactt
     3421 gagcgccttc tcgaagacat ctcgccagat gctccctgcg gtgggaatct ggaatacgac
     3481 agcgattttg tccttctcga gcagagcgcg cgcggcaagc cggaacagca gttcggcaac
     3541 acgatcattc ccgccgagga gcccgactgg gccgcggtgc ggcgcatcgc gctcgacctc
     3601 tccaagcgca cgcgcgatct gcgcgtcatg gactatctgg cgcgggcctc gatcagaacg
     3661 catggttatg ccggcctcaa cgacgtcctg acgctgctgc ggcgcgcact ggagcgctat
     3721 tgggagccca tccacccgca gctcgatccg gatgacgaca acgatccgac ggcccgggtc
     3781 aacatcatcg gctcgctttg cgatgcccag acgatgctcg ccggcatccg tatggcgccg
     3841 ctcgtccagt cgcgcgcctt ggggcgtttc ggattgcgcg acatcagcat cgcctcgggt
     3901 gagacgcagc cgcccgcggc gatggacgcg ccgccgacca tgcaagcgat cgaaggggcg
     3961 tttctcgata gcgcgatcga agagcttcag gcgactgccg acgccgtccg ctcggcgctc
     4021 gagcagacgc gcgccatcga gggtgtcgtc acggagaaag tcggcaccgg taacgccgca
     4081 agcctcgatc cgctctccgc cgtgctcaaa gacgcaagca gggccatcga gacgcggctc
     4141 gcggctcgcg gcgtcggcac cgtcgccacc gctaccgagg tcgtagcagt caacggacat
     4201 gcgacgaacg gcagcgcggc ctccttcgcg gttgccggcc agctgcagag ccgcgacgag
     4261 gtcatgcggc tcttggacaa gatctgcgag tactatgcgc gctacgagcc gtcgagcccg
     4321 gtgccgctgc tgctgcaacg cgccaagaaa ctggtgccga tggatttcat ggaaatcatg
     4381 aaggatctga ttccggacgc ggttgcgact gccgagatgt atcgcgggcg gttgccggaa
     4441 tgaggcagcg tcgcggcggc ggcgctgacg gacgaaactg gaatggcgca ggccctgcgc
     4501 cggaggaaca ggaggacttg agatggcaaa gatgagcagt cagaaattca ttgcgcgcaa
     4561 ccgggcgccg cgcgtccaaa tcgagtatga cgtcgaagtg ttcggttcgc agaagaaggt
     4621 gcagctcccc ttcgtgatgg gagtcttcgc cgatctctcg ggcaagcccg cggaagcgct
     4681 gccggcggtc gccgaccgca aggccgtgct gatcgatgtc gacaatttcg acgaccggct
     4741 caagtcgatg aagccgcgcg tcgccttcca ggtgcccaat acgctcaccg gcgagggcaa
     4801 tctgaacgtc gagctcacct tcgagagcat ggacgacttc tcgccggccg cggtggcccg
     4861 caaggttggc gccctcaacc agcttctcga agcgcgcagc cagctcgcga atctcgtcgc
     4921 ctacatggac ggcaagagcg gagccgagga gctcatcggc aagatgctga cggatccgac
     4981 gctgctcaag gcgatcgcag cggcaccgaa acctcaagac gcgcaaccgg aagccggagc
     5041 gcaggagtaa tcccccatgg ctgaaacaca ggaaattggc cagacgcagg ccggcacggc
     5101 ggagttcagc gatttcggcg cgctcctcaa caaggagttc cggccgaaga gcgagcatgc
     5161 ccaggaggcc attcagaacg ccgtcaagac cttggcggcg caagcgctgg aaagctcggt
     5221 cgtcgtctcg aacgacgtcg tcaacaccat caatgcaatc atcgccgagc tcgaccgcaa
     5281 gctcaccgag cagatcaacc tgatcatcca ccatgcggac taccagaagc tcgaaggcgc
     5341 ctggcgcggc ttgagctatc tcgtcaacaa taccgagacc gacgagatgc tgaagatccg
     5401 cgtgctgaac atctccaagc aggatctcgg caagaccctg cggaagttca agggcacggc
     5461 ctgggaccag agcccgatct tcaagaagat gtacgaggag gaatacgggc agtttggcgg
     5521 cgagccttat ggctgcctcg tcggcgacta ctacttcgac cagagcccgc ccgacgtcga
     5581 gctcctgaac aacatcgccc aggtgtccgc cgcggcgcac tgcccgttca tcggggcggc
     5641 cgcgccgtcg ctgatgcaga tggattcgtg gcaggaactg agcaatccgc gcgacctcgc
     5701 caagatcttc tcgacgccgg aatacgcggc atggaagtcg ctgcgcgact ccgaggactc
     5761 ccgctacatc ggtctcgcca tgccgcgctt cctcgcccgg ctcccctacg gcgccaagac
     5821 ggaccccgtc gaagggttcg acttcgagga agtgaccgaa ggcggcaagc acgacaagta
     5881 ttgctgggcc aattcggcct atgcgatggc cgtcaacatc aatcggtcgt tcaagctcta
     5941 tggctggtgc tcgcggatcc gcggcatcga gtccggcggc gccgtcgaag gcctgccggc
     6001 gcataccttc ccgaccgatg acggcggcgt cgacctcaaa tgcccgaccg aggtcgccat
     6061 cagcgaccgt cgcgaggcgg aactcgccaa ggtcggcttg atgccgctca tccacaagaa
     6121 gaacaccgac ttcgccgcct tcatcggcgc gcagtcgctg caaaagcccg ccgagtacga
     6181 agatcccgat gcgacgtcga acgccaatct ctcggcgcgg ctcccttatc tcttcgccac
     6241 ttgccgcttc gcgcattacc tgaagtgcat cgtccgcgac aagatcggct ccttcaagga
     6301 acgccccgac atgcagcgct ggctgcagga gtggatcatg aactatgtcg atggcgatcc
     6361 ggcaaattcc agcgaagaga ccaaggcgag aaggccgctc gccgcggccg aggttgtcgt
     6421 cgaggaagtc gagggcaatc ccggctacta ctcgtcgaag ttctaccttc ggccgcacta
     6481 ccagctcgaa gggcttaccg tttcactgcg tctcgtctcg aagctcccct cggccaaggc
     6541 cgcgtaaccg tcacgcatac ctgatctcta gacccaaaga ggaatacgca catggcaatg
     6601 gacatcttca tcaagattgg cgatctcaaa ggcgaatcgg ttgacgacaa gcacaaggac
     6661 gaagtccagg tgctggcgtg gagctggggc atgtcccaga gcggcaccac gcattcgggt
     6721 acgggctccg gcgggggcaa ggggagtttc caggacctgt ccttcacgca ctatatcgac
     6781 aaggtctccc cgaacctgat gctcgcctgc gccaacggca agcacttctc cgaagcgctg
     6841 ctgacggtgc gcaaggccgg cgagaagccg ctcgagtacc tcaagatcac gatgaccgac
     6901 ctgatcgtca cgtccgtgac gacgggcggc agcggcggcg aggaccggct caccgagaac
     6961 gtctcgctca acttctccaa gttcaaggtc gagtacacgc cgcagaagaa ggatggcagc
     7021 ggcgacgccc ccatcacgat cggctgggac atcgccggca acgtgaagac aggctgatcg
     7081 caagagacgg ctggggtggg gcagcactgg ccgtagcggt gagggctgcg ggaatgggag
     7141 agtggaatgg gaatggagga ctggctgcgt ggcggtgacc ccagcaccgc cctgagcgat
     7201 gtgcaggaac aggttcgcaa gaacccggcg aatgccaagc tgcgcatatt cctgttccaa
     7261 ctcctggcga tcgagggccg gtgggaccgc gcattgaccc agctcaaggt agccggcgac
     7321 ctcgacgccg gcagccttgc gatggtgcag acctatcgcg aagcgctggc ctgcgaggcg
     7381 ttgcgcgacg agatctttgc cggcacgcgc tcgccgctgg tattcgggca gccggagaat
     7441 tggatcgcgc tgctgatcga ggccttgcgc ctcgcggcgg tcggcaaggt cgagcagtcg
     7501 cagcagatcc gcgccgaggc tttcgacgcc gcgccgacca cgcgcggcac gatcggcgat
     7561 caggctttcg aatggatcgc cgacgccgac cagcggctcg gcccggttct cgaggccatc
     7621 atcaacggac gatactattg ggtacccttc caccgcatcc gcgccgtccg cttcgacgcg
     7681 ccggcggatc ttcgcgacgt cgtgtggatg cccgcttatt tcacctgggc caatggcggc
     7741 gaaagcgtcg gtctcatccc gacgcgctat ccgggctcgc aatcgagcga caatccgcat
     7801 ctcaggctct cacggcggac ggagtggatc gagcgcggcg ccgatctctt catcggcatg
     7861 ggacagcgca tgctggcgac cgatgccggc gagcacgcga tcatggattt gcgcgcgata
     7921 aagctcgaca cggccgaaga gtcggcggac ccgggcaccg cgcaaccggc cgcgatggcg
     7981 tagcggcgca gggccatggc cgaactcacg cagcaagagc gcctgcagcc ctgcctgttg
     8041 gaccgcctca ccgacgacga gcctgagaag aagcaggaag cgcgcgacca gcgggtcgtt
     8101 tcgctgcgcc ggctgcgcga aggcgtgttg cgtgatctcg gctggctgct caatgccacc
     8161 aacctcgcgg tgctcgagga tctggccgac taccccgacg tcgcgaaatc ggtgctgaac
     8221 tatggcatgc cggcgctggc cggcacgctc gtctcgggca tcgacgtcaa tgacttcgag
     8281 cgcaagatct atcaggcgat cgtcgatttc gagccgcgca tcctgcgcga taccctcaag
     8341 gtgcgcctgg ccatctcggg cgatgagatg tcgcacaacg ccatgacttt cgagatcgag
     8401 ggccaactct gggcgcagcc cgtgccgcag cggctctatc tcaagaccga gatcgatctg
     8461 gagctgggcg aggtcaagct gtcggattac tccggcggcg cgagcagcgg ctgatggacc
     8521 cgcgcctcct caaatactac aacgccgagc tccagcattt tcgcgggatg tccggcgagt
     8581 tcgcggccga gttccccaag gtggcgggac ggctcggtct cgacagcttc gaatgcgccg
     8641 atccctatgt cgagcggctg atcgaggggt tcgccttcct ggccgcgcgc gtgcgcctca
     8701 aggtcgatgc cgagtttccc cgcttcaccc gccatctcat gcagatggtg tatccgcatt
     8761 acctctcgcc gaccccttcc atggcggtcg tgcagttccg tccgctgccg gatgaggcgg
     8821 cgcttgccga aggctcggtc gtgccccgcg agaccgtcat gaagacgttg ctcggcaggg
     8881 gcgagcagac gccctgcgaa tatcgaacgg cgcatgacgt caccttgtgg ccgctcgagc
     8941 tcgtcgacgc cgaatatatc ggcagcaccg cggccgtcat cgcgctcggc gtcaccgaag
     9001 tccaaggcgt gcgagcggcg atccggatca agctcaagac cacgggcgtt ctgaccttcg
     9061 ataagctcgc gctcgactcg ctgccgctct ttctgcgcgg cagcaacgag ctgccggtgc
     9121 agatctacga gcagctcatc gccaacgtcg cggggatggt ggtgcggccg gccacgcggc
     9181 cgatcgcctg gcaacatgtg gtgcgcgatg agccgacgcg gccgctcggc ttcgacgcga
     9241 aagaagcgct gctgccctat caggcgccct ccttcgacgg ctatcgcctg ctccatgagt
     9301 atttcgcgtt tccccagcgc tatctcttcg tcgagctgac gggcctccgg cccgccgtgc
     9361 ggcgctgcac gacgaacgag ctcgagatca tcattctcct gaagcgccag gcgccgcgcc
     9421 tcgacggcgc gatcagcaag gaggaattcg cgctcttctg ctccccggcg atcaatctct
     9481 ttccgcggcg cgccgaccgt atccatctca ccgaccggag cgacgagcac cacgtcgtgg
     9541 tggaccgcac gcggccgatg gatttcgaag tctatgccgt gaccggcgtc accggcttcg
     9601 gcgtgggcaa cgagaaccag caggaattcc tgcccttcta tgcgctcacc gacctcgccg
     9661 ccatggcgga ggcgcgcgcc ttctttgcca cgaaccgcga gccgcggctg ttgtcatcgc
     9721 ggcaacgcgc gcgcggcccg cgctcgagct atatcggcag cgagctcttc atctctctgg
     9781 tggacggcaa cgaggctccc tatcgccacg acctgcgcca gctgggcgtg gagaccttgt
     9841 gcacgaaccg cgacctgccg ctcgacgttc cgatcggcaa gggtccgacc gatttcatct
     9901 tgcagctgag cgcgcccgtc gagagcgtcc gctgcgtcgc cggtccgagc aagcccaggc
     9961 cgtccttcgg cgagggcgac gccgcgtggc ggctgatcag ccatctgtcg ctgaactacc
    10021 tgtcgctcgt cgacagcaac cccgagcagg gggccgcggc actgcgcgaa ctgctgtcgc
    10081 tctatgcgga tctcgacgag ctccatatca cccggcagct cgaggggctg cgctcgatca
    10141 gcacccggcc ggtgacgcgc cgcgctctca tccccggccc catctccttc tgccgcggcc
    10201 ttgagatcac ggttacctgc ggcgaggaat cgttcgaggg caccggcgtc ttcctgctcg
    10261 gcgccgtgct cgaccggttc tttgccaaat atgtgtcgat caactccttc acagaaacgg
    10321 tcctgcgcac gctgggccgc ggcgagatca tgcgatggcg gacgcggatc ggacagcggc
    10381 acacgctcta gccctgctgc gcaacctgca ggagaagccg tatgaattcg gcttcttcca
    10441 ggccgtgcgg cggctggaga atctctatcg cgacaagccg cgcaccggca cgtcggttca
    10501 tgcctccgat gatccgatcc gactcgcaca ggaaccgtcg acggcattcg cgccctcgac
    10561 tcttgcgcgg ttcacgccag gagaaggcaa cgcccggccg cggctctcgg gctacttcct
    10621 cgggctgctt gggcccaacg gcccgctgcc gatccatctc accgaatatg cgcgcgagcg
    10681 gatgaggaac ctgggcgacg ccacgttcac ccgcttcctc gaccttttcc atcaccgcat
    10741 gctgagcctc ttctatcgcg cctgggcgat caacgagccg gcggtgagct tcgaccggcc
    10801 ggaagacgat cgcttcgcca tctatgtcgc gtcgctcttc ggcatgggca gcccggcgct
    10861 gcgcaaccgc gatgcgtttc cggacctggc gcggctgcgc tatgccgggc tcttcgcgaa
    10921 ccagacgcgc cacggctccg ggctctgcgc cgtcctgagg gatttcttcc gcatgcccgt
    10981 ggcgatccgc gagttcacgg cgggctggct gcatctgggc gaagacatgt ggtggcgcct
    11041 cggccattcg cgggccagcg gcacgctcgg acaatccgcg accatcggtg cccgcgtctg
    11101 gagctgccat cacaagtttc gcatcgcctt cggccctttg accctcacgc aatatctgcg
    11161 cctgctgccc ggaggcgaga gcttgcgccg cctcgtgccg ctcgtgcgca gctatgtcgg
    11221 cgacgagctc gattgggatg tgaatctcat cttgaagcgc gaggagatcc cgccgctcga
    11281 gctgggccgg caagggcgcc tcggatggac gacatggctg acggcgccgc ggcgcacggg
    11341 cgacgccggc gacctgcggc tcaatccgct cgcggccgca aggtgagcgc catgactcga
    11401 gcaaaagggt ggggccagtc cgttccggcc attcatgagt tgaggagtac gttatgaccg
    11461 agatcagccg cgccgcctta ttcggcaagc tcaacagcct gggctaccgg gccatcgaga
    11521 gcgccaccgt cttctgcaag atgcggggca acccctatgt cgagctggtg cattggctgc
    11581 accagatcct gcagcagcag gattccgaca tccaccgcat catcaagtat ttcggcctcg
    11641 atgccgggcg gctcgcgaag gacgtggttg atgcgctcga ccggctgccg c
//
LOCUS       DASYNS010000440        28236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1359760, whole genome shotgun sequence.
ACCESSION   DASYNS010000440 DASYNS010000000
VERSION     DASYNS010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 28236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28236
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359760"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VGP50_13660"
     CDS             <1..235
                     /locus_tag="VGP50_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028475.1"
                     /translation="YAVFCKETDPAQRPVTMLGVWLAMVTTLLSFGLLAFSRVFAVQA
                     FGATLLVGTLLAFLVAPLTGDSSTVRARMIREK"
     gene            312..872
                     /locus_tag="VGP50_13665"
     CDS             312..872
                     /locus_tag="VGP50_13665"
                     /inference="COORDINATES: protein motif:HMM:NF023089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HEV8028476.1"
                     /translation="MALVLGSISGACTAPPLEPGAYIAPELRFALPRPRDLGHSVNAV
                     QLVTAHYRGDVQMFEAHLSVSPDRMTLIGLDPFGRRALTVTLTDTGIDIDKAPWFPQA
                     LRPENILADVTIIYWPEEAVRRGLARTDAVLRSDAHGRSIIADGREVVRVDYAPTAGD
                     AWAGTAHYRNDAFGYELDLRSAVDAP"
     gene            869..2056
                     /locus_tag="VGP50_13670"
     CDS             869..2056
                     /locus_tag="VGP50_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEV8028477.1"
                     /translation="MTLYLNALGIVNALGRGKRQVAANLFGGSRAGMVLRDDLIPGRS
                     VRVGVIDGPLPSLPSHLADFDCRNNRVMLAALEEIEAAVMAARRRYGGERIAVVVGTS
                     TAGIAEGEQALRARLTEGAWPRSYHYSQQELGNLSAFVAAYFDLSGPAYTIATACSSS
                     GKAFASAQRLIEAGICDAALVGGADTLCRLTLNGFNSLEAIAADYCNPFSRNRDGINI
                     GEGAAVFLLSREHSAVALLGVGESSDAHHVSAPDPAGRGALHAMRRALEAAGLPAASV
                     DYVNFHGTATTLNDVMEGRVVAQLFGGDMPCSSTKTMTGHMLGAAAAGEAGMMWLALD
                     PEYSGGALPPHLWDGAADPEIPALNLVPLGTRIPLGETCTMLSNSFAFGGSNVAVLLG
                     RGW"
     gene            2058..2519
                     /locus_tag="VGP50_13675"
     CDS             2058..2519
                     /locus_tag="VGP50_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028478.1"
                     /translation="MKPCRYGVAELLPHRPPMILLDEILGYDEGSLSAAVRVGPASLF
                     VESGAVPAHIGLEYMAQACGAFAGVVARERGAPVRIGFVLGTRQYRAHVPSFRCGDRL
                     VVTVSVLYQDEQMGAFDCRIEIAGRLAAEAQLSVYQPDPAQLAADGRVAAP"
     gene            2516..3241
                     /locus_tag="VGP50_13680"
     CDS             2516..3241
                     /locus_tag="VGP50_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HEV8028479.1"
                     /translation="MSKTILVTGASKGIGRAIALRLARDGFTVVVHYRGDRAGAAATL
                     GALQEAKGTGRILGFDIADRTECRERLAADVAEHGVYYGVVLNAGIARDNAFPALEDD
                     EWDSVIDTDLGGFYNVLRPLVMPMVSARRGGRIVTLSSLSGLAGNRGQVNYSAAKAGI
                     IGASKALAVELAKRQITVNCVAPGLVETQMTEGLPLDEIVKTIPMRRLGRAEEVAAVV
                     SFLCSDDASYVTRQVISVNGGLL"
     gene            3238..4464
                     /locus_tag="VGP50_13685"
     CDS             3238..4464
                     /locus_tag="VGP50_13685"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEV8028480.1"
                     /translation="MRRRVAVTGMSGVTALGNDWPTIRAKLAQGRTAVKRMSDWDRYG
                     SLNTRLAAPVEGFDVEGRYDRKKLRTMGPVSRMAVYASEQALGDAGLLGERCLASGRT
                     GVAYGSSFGSHQAVLGFAELMLSGNSTKLTGTNYVQMMSHTAAVNIGLFFGLTGRIVP
                     TSSACTSGSQGIGYAYEMIRHGQQDIMLAGGAEELSVGDAAAFDTLYATSVRNDAPQT
                     TPRPFDRDRDGLVIGEGAATLVLEELEHAKARGARIHAEVLGFGTNSDGRHITQPAPE
                     TMQQALRLALADAALAPEAVGFVNGHGTATDWGDVAESNATAAVFGSRMPIHSLKSYF
                     GHTLGACGSIEAWLGITMMNEDWFVPTANLANVDERCAPLDYVMGEPRRLAIEHLMSN
                     NFAFGGINTSLIFKRM"
     gene            complement(4613..5341)
                     /locus_tag="VGP50_13690"
     CDS             complement(4613..5341)
                     /locus_tag="VGP50_13690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8028481.1"
                     /translation="MRQLAHFADVRSPSSVYVPESRFGLWFLGTGIWETHVLARAIDD
                     LSRLIGGRVIAAPVIVDVGCGWGHSFKLLQDRFAPHRMVGIDIDPAMLEAAGERTAKL
                     GLSVEFRLGASASLPLAGETADLIFCHQTFHHFVDQEGALREFRRVLKPGGLLLFAES
                     TKAYIHSWIIRLLFRHPMEVQRTAPEYLAMIRASGFAVAPGAISHPYLWWSRPDLGVL
                     ERWFGVTPRAGHEETLLNLVAVRQ"
     gene            complement(5410..5742)
                     /locus_tag="VGP50_13695"
     CDS             complement(5410..5742)
                     /locus_tag="VGP50_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028482.1"
                     /translation="MRAPASMLLLVTLLALGGVAAAAQSSTPAPPDQATPTPGAAPPG
                     SSGSSTEPGDGAPNAPGDALSRSHGVIEPPATGDRSVVPPPRTGSRSTPVIPPPGTPG
                     GNQDVQPK"
     gene            complement(5774..8143)
                     /locus_tag="VGP50_13700"
     CDS             complement(5774..8143)
                     /locus_tag="VGP50_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV8028483.1"
                     /translation="MDDASDGAALALTRFGIGQPVPRQEDPKLLRGEGRYADDVNLPG
                     QAYAVMVRSREAHGLIRRVDGANARAMPGVLAVYTAADLGAYGALKPGILVPNRDGSA
                     MRPPPRPALPDERVRFAGEAVAFVVASTPAQAKDAAEAVAVEIDPLPVVTTPEAAVAP
                     GAPQLHPEAPGNVAVDYHYGDTAAVDAAFARAAHRTRLTLTNNRIVVNPLEPRSAVAQ
                     YAAAADRWTLHLGCQGVFGLRNTLARDVLAVPPERVRVLTGNVGGSFGMKASVFPEYV
                     CLLHAARALGRPVKWTDERSESFLSDHHGRDHIVNAELALDATGHFLALRLTGYGNAG
                     AFVVAVLPYTVNAVKNVVSVYRVPLLDVSTKGVFTNTTPTGAYRGAGRPEGNYYMERL
                     IDRAAVEMGIDRIELRRRNHIGADEMPFKAASGMSYDSGEFTAVLDEALSLADWNGFA
                     ARRAQSLARGKLRGLGIGSYLEVTAPPSKELGGIRFEADGSVTIVTGTLDYGQGHASP
                     LAQVLVDRLGIPFEQIHLLQGDSDALAAGGGTGGSRSMMASGTALVEASAQVIERGKE
                     IAGFVLEAATADIVFARGRFAIAGTDRSVGLMELAAQLRAGLQPPPELPRALDVAHVS
                     DNPPSSFPNGCHIAEVEVDPETGAVAVVSYAMVNDFGTLINPPLVEGQAHGGIVQGIG
                     QALLERTVHDAAGQLLTGSYVDYAMPRANDAPMFRFASHPVPAKTNVLGVKGCGEAGC
                     AGSLPAVMNAVVDALYACGVDHIDMPATPERVWRAIAAARGNPVGGPGR"
     gene            8351..8794
                     /locus_tag="VGP50_13705"
     CDS             8351..8794
                     /locus_tag="VGP50_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEV8028484.1"
                     /translation="MDLYQLIKQDHQKVRRLFERLAEADSGTPSQARLFAELKRELDL
                     HSTAEEKYFYPALQQYDEAKNLIEEAIEEHGDVKATLEELDHSDKEDERWAEELSELQ
                     QDVEHHIEEEENIIFPIAAELLDAEQADAIARDIQKEKAAAQGTA"
     gene            8960..10834
                     /locus_tag="VGP50_13710"
     CDS             8960..10834
                     /locus_tag="VGP50_13710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028485.1"
                     /translation="MKRPRLNLSPARIYPLLDWFIPPAVKADREMLQPARMFLISHLF
                     GPFMANTIVIYLYVIDPKPGFGLAVLALALNAFWAFPFALKYLGHYQLLAMLSVEDLI
                     FSIFWGSYHYGGVNSPFLPWLLVVPLLAFFYLGPATHLRLPALLLIAINFGFFYLSYA
                     LGHSFPEHVPLGALTGIGLISTLCSAVYVSMMALSYANTVANQSELEREAQNHRATAI
                     KLREAKEEAEAANRAKSEFLAKMSHELRTPLNAVIGYSEMLLEDAEIEGSEEQQVEDL
                     RKINGAGKHLLSLVTDVLDLSKIEAGKMELFNERFDLDGLVDDVAATCQPLCTKNGNE
                     LVVERKGELGMAEGDVTKLRQAVLNILSNAAKFTRNGRVTLTVAREPRVGGAWIRIDV
                     RDTGIGISPENLGKLFQNFNQAEASTASRYGGTGLGLSLTQKLCRLMGGDVMVESQLG
                     QGSCFTIRLPGSLVDSVERRADLDWTTEAIRADAAGRQNTVLVIDDDPAMLDLMRRIL
                     TREGFRPVLSDDPRKTLSLAHSAKPSLIFLDVLMAGANGWEVLRALKADAELEACPVV
                     MLTIVDDRRTALALGAAEHLLKPIDRDVLTRVLDRFCPRDRAARTPTGDDNQSVAANA
                     "
     gene            10827..11204
                     /locus_tag="VGP50_13715"
     CDS             10827..11204
                     /locus_tag="VGP50_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028486.1"
                     /translation="MPKILLVEDNEMNRDMLGRRLQRHGFTLCFAADGPTGITMAGTE
                     MPDLILMDIALGEMDGWEATRRIKANPPTSRIPIIALTAHALASDRDKSVEVGCADFD
                     TKPVDLPRLLAKINTCLANGKTQ"
     gene            complement(11244..11639)
                     /locus_tag="VGP50_13720"
     CDS             complement(11244..11639)
                     /locus_tag="VGP50_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028487.1"
                     /translation="MQANTNRGRLLAVDDSADSADLVARVAVKCGYDAQAISDPKSVR
                     ALVVSWKPEVLTLDLCMPEADGIDLLSMLEETGFAGLLVIISGQDGWFRKAASRLAEV
                     RGLKVVDDLQKPVDINALRQLLSRLRQDS"
     gene            complement(11645..12985)
                     /locus_tag="VGP50_13725"
     CDS             complement(11645..12985)
                     /locus_tag="VGP50_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV8028488.1"
                     /translation="MTDSTVPIEALTGSMRDFREPHGPDLLGRVEQFFRWEALRQQHD
                     LWPYSRSTDEAVAPVCAARDESGERFTGVNFAAQDYLSLASHPAVHETAKAAIDQYGV
                     HSAGTAALLGNTRTSLALERVLADFLHMEHVLLYPTGWAAGFGVIKALVRPDDHVVMD
                     ALSHSCLKEGATAATRNVHLHPHLNVLAVSRYLSRIRARDTKNAILVVTESLFSMDSD
                     TPDMAALQDLCREFGATLLVDVAHDLGNIGDDGKGHIGLQHMLGLVDIVMGSFSKTFA
                     SNGGFVACNQRAVREYLRFYSAPHTFSNALSPIQSGIVLKAFEIVKSAEGRALRTQLM
                     TNVEYLRQSLQHAGFEVLGEPSAIVPAMMRSESLARLVARRLPSLGVLANMVEFPAVA
                     KNSSRFRFQVMAKHSRNNVDQAVSRLRVAYDEAQGALRSYPQLAAKGAVASAGD"
     gene            complement(13191..14471)
                     /locus_tag="VGP50_13730"
     CDS             complement(13191..14471)
                     /locus_tag="VGP50_13730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEV8028489.1"
                     /translation="MISETARSSRILVIDDDPVSVELVKRVLTRAGFHTVDSTSEPSI
                     ALSLLREVKPHVVLLDLYMPGMDGFAVLDMLRADADRVGCSIIMLTGEADHDVQVAAL
                     QRGASDFVTKPFEAPVLVARVAGAAEMRALQRQMRDQNQRLQEAVNARTQRLQQALDV
                     LTGAESELKRSLVRSEADAHIRAEILAELAHELRTPMSAVCGFSDMMRHQEFGPLPPR
                     YREYAEDIHRAGLNVLQVLNGFLDLAKAEARQEPLAVSAVDVGSVVNESVKLISAQAE
                     KAGVALKVVVKPGVEEVQSDRAKLMQIVVNMTSNAVKFTPAGGTVTVEVGPDAGGGAY
                     IIVVRDTGIGIAAKELPEMLRPLDQLRTASGKPKGTAGFGMPLTRQYVEMLGGTLAIV
                     SQPRQGTAVTIRLPKSLPEERARASGITADTQGE"
     gene            14563..14961
                     /locus_tag="VGP50_13735"
     CDS             14563..14961
                     /locus_tag="VGP50_13735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028490.1"
                     /translation="MMNTPALQDKRVLIVDDDEPTRSLVRRVLKRMKIANLQEAAGAE
                     QAIACLNESSEDIDLVICDWNMPGMSGIELFDRVHALRPELPFLMLTGRGDATSVIAA
                     KKAGIPAYIVKPIFPQELKTKVSFLLMKDA"
     gene            complement(14977..16440)
                     /locus_tag="VGP50_13740"
     CDS             complement(14977..16440)
                     /locus_tag="VGP50_13740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV8028491.1"
                     /translation="MLKRLSAIGAVIYRKSDWFIPETLRTDAATLERARIFVFSHLFG
                     PFLGQFISIYLYILDPHPGMAYWAIVGSITSFWTLPFWLKVTGRLLPLALFTVENLTF
                     VTLFGSYHYGGVSSPLLPWLLNALLLLFFYLGDRPKLRYLVLALFALNCVGFYLAYAV
                     NKSFPEHVPIAAMSGLGLSSVLCATVYMSMMAIYYANVVASQSSLEQEVQKHRATAIQ
                     LHEAKEEAEAASRAKSEFLAKMSHELRTPLNAVIGYSEMLLEDAETDGRKQQQITDLR
                     RINSAGKHLLSLVTDVLDLSKIEAGKMDLSVQSFDLNTFIDDVVATCRPLVMENGNDF
                     VVEREGALGVIIGDATKLRQSALNLLSNAAKFTNGGRVVLRAMRERTPQGDWISLAVH
                     DTGIGISHENLPKLFQNFNQAEASTSSRYGGTGLGLALSQKLCRMMGGEITVESDLGR
                     GSSFTIRVPAVISPPPRLASVDGAAAPLFSTAAAAAF"
     gene            complement(16512..17045)
                     /locus_tag="VGP50_13745"
     CDS             complement(16512..17045)
                     /locus_tag="VGP50_13745"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8028492.1"
                     /translation="MEYDLRPPALRIRPAAPADVPALFRLKQALTKAEGNEAVLRATA
                     ADWLRDGFGTQARFASLVAESGGTIVGMLTYSEFYLTALADVVLSIQDLFVEPAHRRS
                     GAGRALLATLAAHAVERRIPLIQLNVQDDNPARPFYRRAGFQHLRGCLTYAVGGRPMQ
                     DLAAGTIIDPTAAVPET"
     gene            17181..18410
                     /locus_tag="VGP50_13750"
     CDS             17181..18410
                     /locus_tag="VGP50_13750"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV8028493.1"
                     /translation="MSAERQIPAKPPKAPAVTPAAGAPPMLGNEEMLLAYVEQLADAR
                     KGQRAMVVHLSRLQGAKPRDKNLQIVAAVLREVTDQFSGRLFALENGDIVVVCKGITR
                     RAIDETIEVLRYLFNDDPVTRAGTEQADFCAMFDLEVGYVDFMFALDEIREAARRSSI
                     TAGQRRRFDPGAIDPPHAGDLFKALQRVELAGMLRRQTVWDMAQDTVPRPFFEEIFVS
                     MDRLQKAIGPAFDLAKDRQLFLYVSRWLDQHVMATVARHQSNVSLRLGFSLGLATLMS
                     PEFDDFDKRGAIGPERAVLEIPLADLLRAPSTFRQVADRLKQRGYRLCIDDVSHQILP
                     YVNLRPLAPDYVKVIWDDALMHLGEEPLREFCAAMTAVGSERIILSRCGRRQAIRVGH
                     AMGIRLFQGWQINQANR"
     gene            18482..20803
                     /locus_tag="VGP50_13755"
     CDS             18482..20803
                     /locus_tag="VGP50_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV8028494.1"
                     /translation="MKFGIGQPLRRHEDLRLLTGKGRYTDDIALPGMVFAAILRSPVA
                     HARLFKVDATAARGMPGVLLVLTGVEVTADQLGNLPCVAAITSRDGRPRHDTPRPILA
                     IGKLRHVGQAIAIVVAETLHQARDAAEAIEVDYDELPPVVDVRAATAPGAPQLFDQIP
                     DNLVFDWDNDMTDAARTDAAFAKAARVIALELVNNRVVANSMEPRNAIADYDSQSDRS
                     TLYTTTQGPHFVRDPLAMAVFKFAKEKLRVVTPNVGGAFGMKAFVYPEQALVIWASRK
                     LKRPVKWQADRSEGFVSDNHGRDHVTRAELAVDDKGRFLGLRVSTLANLGAYLSPLGP
                     FIPTRSSDLVAGLYTTPAIHINVKGVCTNTLPVCAYRGAGRPEAAYLMERLVDVAARD
                     LGMTPDALRRINFIPASAMPYTSATKIVFDSGEFEKVMDLAMNAADWTGFASRRTETE
                     RRGRLRGRGMATYTERCGGGFPEAASIEFKDSRVELVMGNIEYGTGLVTAYKQIVADW
                     LGLDIDRIEVVMGDTDRTPSGLTGGSRALAVSGSALYECSQEIIEKGRMLAAHFLETA
                     PADVQFAEGNFFVPGTDLRLDLLDVATAARDPTKLPPGMTPGLDTTKQRTPLANTFPN
                     GCHICELEIDPETGVVAIDRYTIVDDFGRTINPLMLEGQVHGGVVQGIGQALLEHTVY
                     DPDSGQLLSGSFMDYAMPRADDVPSFAFATHNVPCTANPLGVKGAGEAGAVGAPPAII
                     NAIVDALHAKTGLRHLDMPATPRRVWEALQRAK"
     gene            20882..22123
                     /locus_tag="VGP50_13760"
     CDS             20882..22123
                     /locus_tag="VGP50_13760"
                     /inference="COORDINATES: protein motif:HMM:NF005720.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HEV8028495.1"
                     /translation="MEVLIIGAGIGGLTLALMLHRAGIPCRVFEAAAEIKPLGVGINL
                     LPHAMKELAGLGLEDTLASRGVLTAEAVFFNRFGQLIYREPLGRAAGYAWPQISIHRG
                     DLQAALVEAFIQRLGKDRLMLGWECVSVASEEGAAVAHFRDPIAKRELPAQRGIAAIG
                     SDGIHSAIRRQLYPDEGGPIYSGVNMWRGVTRWPPILSGASMIRAGWFAHAKIVLYPI
                     RHDVDAEGRQLVNWVVEIATPRYEGWNWSRPATLDDFIAPMTDWHFDWLDVPEFLRRS
                     DLMLEYPMVDKDPLPRWSFGRVTLLGDAAHPMYPRGSNGAGQAILDARALADCLATSS
                     DAVAALAAYDERRRDATARVVRANRAAPPDAILGEVYRRTGDKPFASIDDVISREELA
                     AITEAYKRVAGYEKQKLSGED"
     gene            22443..24128
                     /locus_tag="VGP50_13765"
     CDS             22443..24128
                     /locus_tag="VGP50_13765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HEV8028496.1"
                     /translation="MNMSTPEQVFRTALSLQFRGRLSEAERLYRTLLKSVPDHLDCLQ
                     NLGNVCIEQGKLEEARRFFQKALNLKPGSADARNGLGTVLQALGRDQEAAVQFEKAIA
                     AEPTFVEARNNLGNVLQRLRRHDEAIAQYRQALALRPEYPLARANLADALAALQRDGE
                     AVEEYEEALALRPDDPAIHYNLGNALQKLDRPADAVRHYERAIALKPDDAHAHTNLGA
                     ALQALNRHGEAIGQYQKALALTPDDANVLWGLGLAELAVGDYEGGWRHHECRWDAPAT
                     GLRPRNFPAPAWQGKERLAGRTILLHSDQGFGDTIQFSRYVPLVAAEGAKVVLEVQRP
                     LVPLLASLQGLSELRAQGEALPAFDLHCSLQSLPFALGTRLETIPAGVPYLNPPADAV
                     ARWRSRLRPPDGSPRIGIAWAGNPAQRNDRNRSIPLALLRPLLAKTPGIVVLQQELRD
                     GDGEILGALSTIALHEGRFADFAETAALISALDLVITVDSAVAHLSGALGKMLWVLLP
                     YSTDWRWPVDRTDNPWYPTARLFRQPSIGDWASVIRSVAAALDRQTRGPSSAP"
     gene            24312..24977
                     /locus_tag="VGP50_13770"
     CDS             24312..24977
                     /locus_tag="VGP50_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV8028497.1"
                     /translation="MRILVVEDDAEAAAYLVKGLTESGHSVDLAAEGRSGLDRARRGT
                     YDAMIIDRMLPGLDGLSIIHALREARNQTPVLVLSALGEVDDRVKGLLAGGDDYVVKP
                     FALNELLARLAALTRRGNAETRLVVADLEMDLLSRKVTRAGREVELLPRELRLLEYLM
                     RNAGHVVTRTMMLEKVWDSHVDPQTNVIDVHVGRLRQKIDKGFARPLLHTVRGAGYTL
                     RPE"
     gene            24982..26415
                     /locus_tag="VGP50_13775"
     CDS             24982..26415
                     /locus_tag="VGP50_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV8028498.1"
                     /translation="MLRLLRTNAFRLAAGYFALFAASVLALLAFVYLSTADFVEKQTE
                     ATINAEIRGLATQYEQNGLHGLIDLINARIAGQQIRATLYLITDDRLNPIAGNLSAWP
                     AATTVRPGWIEFPVEARDRDARETGSALASIFVLRDGYRLLVGRDLRDVTAFRSRFNH
                     TLGWTALATVALGLAGGLFSTRNIMRRVEAMNRTSENIIRGDFSQRVALDGSGDEFDQ
                     LSANLNAILERIERLMTGMRQVTENIAHDLRTPLARLRARLEITLLERPDADRSLEAL
                     RETIAEADRLLATFNALLRIAEAEAGARRHTLALVDLAEIARSMAELYAPVAEEKGLA
                     FTVETDPVIVVRGDRLLLSQMIVNLVDNALKYTSAGTVALTARRQGTLVRIEIADSGP
                     GIPADRREVVFDRFVRLEGSRSTPGNGLGLSLVRAVATLHGGDVSLDDAYPGRSPRGL
                     KAVFTLPASEGSMAPRLPPVALLRKPA"
     gene            complement(26455..26844)
                     /locus_tag="VGP50_13780"
     CDS             complement(26455..26844)
                     /locus_tag="VGP50_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV8028499.1"
                     /translation="MAKLSIRVDLAPSGAVGPGKIRLLESVDATGSISAAGRSMNMSY
                     RRAWLLIDDLNRLFSRPVVATRLGGAAGGGAELTAFGKRLVRHYRDMENEAHAALKPH
                     LNAFQTALAKKRLRRSTSRSTAESDDT"
     gene            complement(26938..27126)
                     /locus_tag="VGP50_13785"
     CDS             complement(26938..27126)
                     /locus_tag="VGP50_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028500.1"
                     /translation="MAKEPMLHRTVLLALGATALLLAGCVSAPGTYPDPNGPRDGDGL
                     LVDPQTGIVLPGQSDVGF"
     gene            complement(27280..27471)
                     /locus_tag="VGP50_13790"
     CDS             complement(27280..27471)
                     /locus_tag="VGP50_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028501.1"
                     /translation="MANDSTSLPSTALLALVLTALLAAGCAAPPGTYPDYDGPRNGDG
                     FLIDPQTGLELPGQADPGL"
     gene            complement(27624..>28236)
                     /locus_tag="VGP50_13795"
     CDS             complement(27624..>28236)
                     /locus_tag="VGP50_13795"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEV8028502.1"
                     /translation="AAANALIGVARAAFYPNVSLDLLGGFQNTGGSKLIAAPFSFWSL
                     GPSVTLPLFDAGLRNAADQAAWAAFNETAANYRQTVLDAFQEVEDNLALLRILAQEAQ
                     EEAAAVQAAEQTLNLSLSLYRGGAVNYLEVVVAQTAALQAELTSLVIQYRRLEASVDL
                     VRTLGGGWQIQDLPDEHAVETADAPAPAEQKSESWWSGLWPKL"
BASE COUNT         4797 a         9282 c         9366 g         4791 t
ORIGIN      
        1 ctacgcggtc ttctgcaagg aaaccgaccc ggcgcagcgt ccggtcacga tgctcggcgt
       61 ctggctggcg atggtcacga cgctcctgtc cttcgggttg ctggccttca gccgcgtctt
      121 cgcagtccag gccttcggcg cgacgctgct cgtcggcacc ttgctcgcct tcctggtcgc
      181 cccgctgact ggcgattcaa gcacggttcg cgctaggatg atccgagaaa aataacgtgg
      241 tcacgcagcg gtttcactct ttgtcgacaa agcgcgcagg ttctcatgtc gggcgcgcgc
      301 agcacctttt gatggcgctt gttctcggca gcatatccgg cgcctgcact gcgccgcctc
      361 tcgagcccgg ggcctatatt gccccggagc tgcgcttcgc gctgccccgt ccgcgcgacc
      421 tcgggcatag cgtcaatgcc gtgcaactgg tgacggcgca ttatcgcggc gacgtgcaga
      481 tgttcgaggc gcatctcagc gtgtcgcccg accgcatgac gctgatcggc ctcgatccct
      541 tcggccggcg ggcgctcacc gtcaccttga ccgacaccgg gatcgacatc gacaaagcgc
      601 cctggttccc gcaggcgctc cggcccgaaa acattctggc cgacgtcacc atcatatatt
      661 ggcccgagga ggccgtgcgc cgcggccttg cgcgaaccga cgcggtgttg cggagcgacg
      721 ctcacgggcg atcgatcatc gccgatggcc gcgaggtcgt ccgcgtcgat tacgcgccga
      781 cggcaggcga cgcctgggcc ggcacggccc actatcgcaa cgacgccttc ggctatgagc
      841 tcgatctccg ttccgcggtc gacgcgccat gacgctctat ctcaatgcgc tcggcatcgt
      901 caacgcgctg ggtcgcggca agcgtcaggt ggccgccaat ctctttggcg ggtcgcgcgc
      961 gggcatggtt ctccgcgacg atctgatccc cggccgcagc gtgcgcgtcg gcgtgatcga
     1021 cggaccgctg ccgagcctgc cgagtcatct ggcggatttc gattgccgca acaaccgagt
     1081 gatgctggcc gcgctcgagg agatcgaggc cgccgtcatg gcggcgcgcc gccgttacgg
     1141 cggcgagcgg attgccgtcg tcgtggggac gagcacggcg ggtatcgccg aaggcgagca
     1201 ggcgttgcgc gctcgcctga cagagggcgc ctggccgcgt tcctaccact acagccagca
     1261 agagctcggc aatctcagcg ccttcgtcgc cgcatatttc gatctgtccg ggcccgccta
     1321 caccatcgcg acggcgtgct cctcgagcgg caaggccttt gcgtcggcgc aacgtctcat
     1381 cgaggccggc atctgcgacg ccgctctcgt cggcggcgcc gatacgctct gccgtctcac
     1441 gctcaacggc ttcaactcgc tcgaggcgat tgccgccgac tactgcaatc ccttcagccg
     1501 caaccgggac ggcatcaata tcggcgaagg cgcggccgtc tttctcctct cgcgcgagca
     1561 ttcggcggtg gcgctgctcg gcgtcggcga gagctcggac gcccatcatg tgagcgcgcc
     1621 cgatcctgcc gggcgcggtg cgctccatgc catgcgtcgc gcactcgagg ccgccggcct
     1681 gcccgcggcg tcggtcgact acgtcaattt ccatggcacg gcgacgacgc tcaacgacgt
     1741 catggagggc cgtgtcgtcg ctcaactctt cggcggcgac atgccctgca gctcgaccaa
     1801 gaccatgacc ggtcacatgc tcggcgccgc cgccgccggc gaggccggaa tgatgtggct
     1861 ggcgctcgat cccgaatatt cgggtggagc attgccgccc catctctggg acggagcggc
     1921 cgatccggag atcccggcgc tcaatctcgt gccgttgggg acgcgcatac ctctcggcga
     1981 gacgtgcacg atgctgagca actccttcgc cttcggcggc agcaatgtcg cggtgctgct
     2041 cggacggggc tggtgagatg aagccctgcc gctatggcgt cgcggaactg ctgccgcacc
     2101 gaccgccgat gatcctgctc gacgaaatcc tggggtatga cgaagggtcg ctgagcgccg
     2161 ctgtccgcgt ggggccggcg agcctcttcg tcgagtccgg cgccgtgccg gcccatatcg
     2221 gcctcgaata catggcgcag gcctgcggcg cctttgccgg cgtcgtggcg cgcgagcgcg
     2281 gcgcgcccgt gaggatcggc tttgtcctgg gcacgcggca ataccgggcc catgtgcctt
     2341 cgttccgttg cggcgaccgg ctggtcgtca ccgtctcggt gctctatcag gacgagcaga
     2401 tgggcgcatt cgactgccgg atcgagatcg ccggcaggct tgcggccgaa gcgcaattga
     2461 gcgtctatca acccgatccg gcgcagcttg cggccgatgg ccgcgtcgcc gcgccatgag
     2521 caagaccatc ctcgtcaccg gcgcaagcaa aggcatcgga cgtgccatcg ccttgcggtt
     2581 ggcgcgcgac ggcttcaccg tggtcgttca ctaccgcggt gaccgcgccg gggccgcggc
     2641 gacgctgggg gccctccagg aggcaaaggg cacggggcga atactcggct tcgacatcgc
     2701 cgaccgtacg gaatgccgcg agcgcctcgc ggcggacgtc gcagagcatg gggtctatta
     2761 tggcgtggtg ctcaatgccg gcatcgctcg cgacaacgcc tttcccgccc ttgaggacga
     2821 cgagtgggac agcgtcatcg acaccgatct cggcggcttc tacaacgtcc tgcggcccct
     2881 cgtgatgccc atggtgtcgg cgcggcgcgg cggccggatc gtcaccttgt cgtcgctctc
     2941 gggcctggcg ggcaatcgcg ggcaggtgaa ttacagcgcc gcgaaggcgg gtatcatcgg
     3001 cgcgagcaaa gcgctggccg tggagcttgc caagcgccag atcaccgtca actgcgtggc
     3061 gccggggctg gtcgagacgc aaatgaccga ggggctgccg ctcgacgaga tcgtcaagac
     3121 cattcccatg cgccgcctcg gccgtgcgga agaagtggcc gccgtggtga gctttctttg
     3181 ctcggacgac gccagctatg tgacgcggca agtgatctcg gtcaacggag gcctgctgtg
     3241 aggcgccgcg tggccgtcac cggcatgtcc ggtgtcaccg cactcggcaa tgactggccg
     3301 accatccgcg cgaagctcgc gcaaggacgc acggccgtca agcggatgag cgactgggac
     3361 cgctatggca gcctcaacac cagactcgct gcgcccgtcg agggcttcga tgtcgagggg
     3421 cgctacgacc gtaagaagct gcgcaccatg gggccggtct cgcgcatggc ggtttatgcc
     3481 tcggagcagg cactcggcga tgcggggctg ctgggcgagc gctgcctcgc gagcggacgc
     3541 accggcgttg cctatggctc ctccttcggc agccaccagg ccgtgctcgg cttcgccgag
     3601 ttgatgctgt cgggcaattc gaccaagctc accggcacga attacgtgca gatgatgagt
     3661 cacacggcgg ccgtgaatat cggcctcttc ttcggcttga ccgggcgcat cgtgccgacc
     3721 tcgagcgcct gcacctcggg aagccagggc atcggctatg cctatgagat gatccgccat
     3781 ggccagcagg acatcatgct ggcgggcggc gccgaggaac tctcggtcgg cgacgccgcc
     3841 gccttcgaca cgctctatgc gacgagcgtg cgcaacgacg cgccgcagac gacgccccgg
     3901 ccgttcgacc gcgaccgcga cggcctcgtc atcggcgagg gcgcggcgac gctggtcctc
     3961 gaggagctcg agcatgccaa ggcccgcggc gcgcgcattc acgccgaagt gctgggcttc
     4021 ggcaccaact cggacggccg ccacatcacg cagccagcgc ccgagaccat gcagcaggcg
     4081 ctccgtctgg cgctggcgga cgccgcgctc gcgcccgaag ccgtcggttt cgtcaatggt
     4141 cacggcaccg cgaccgattg gggcgacgtc gccgaaagca acgcgacggc cgccgtgttc
     4201 gggtcgcgca tgccgatcca ctcgctcaag agctatttcg gccatacgct gggcgcctgc
     4261 ggcagcatcg aggcatggct cggcatcacg atgatgaacg aagactggtt cgtgcccacc
     4321 gcgaacctcg ccaatgtcga cgagcgctgc gctcccctcg actatgtgat gggcgagccg
     4381 cggcgcctcg cgatcgagca tctgatgtcg aacaacttcg ccttcggcgg catcaataca
     4441 tcgctgatct tcaagcgcat gtagcgaagc accacgggcg aaagaagaga gagaaaacgc
     4501 agagggcgcg aaggttgcgc ggagggcgca gaggatgttc gttcctccgc ggtgccactg
     4561 cgtcctctgc gttcctcttt aattttctcc gaacgcctct ggccgatctt ttttattgcc
     4621 gcacggcgac gaggttgagc agggtttctt cgtgaccggc ccgcggcgtc acgccgaacc
     4681 agcgctcgag gacgcccaga tcgggccggc tccaccacag atacggatgg gagatcgcgc
     4741 ccggagccac cgcgaaaccg gaggcgcgga tcatcgccag atattccggg gccgtgcgct
     4801 gcacctccat cgggtgacgg aacagcagcc ggatgatcca cgagtggata taggccttgg
     4861 tcgattcggc gaagaggagc aggccgcccg gtttgagcac gcggcggaat tcccgaagcg
     4921 cgccctcctg gtcgacgaaa tgatggaagg tctgatggca gaagatgaga tcggcggtct
     4981 cgccggcgag cgggagagag gcgctcgccc ccaggcggaa ttccacggag aggccgagct
     5041 tcgccgttcg ttcaccggca gcctccagca tcgccgggtc gatatcgatg ccgaccatcc
     5101 ggtgtggcgc gaagcggtcc tggagcagct tgaacgagtg tccccagccg cagccgacat
     5161 cgacgatcac gggagccgcg atgacgcggc cgccgatcaa gcggctcaga tcatcgatgg
     5221 cccgtgccag aacatgggtt tcccagatcc cggtgccgag gaaccatagc ccaaagcggg
     5281 attccggcac gtaaacgctc gaaggcgagc ggacatcggc aaaatgcgcg agctgacgca
     5341 tgggctcccg ttccgcgacc gtcgcggaaa acgaggttag tacagcaggc gctgcgcttc
     5401 cagcctcggc tacttcggtt gaacgtcctg attgccgccg ggggtgcctg gcggcggaat
     5461 gaccggcgtg ctgcggctgc ccgtgcgggg cggcggcacg acactccggt cgccggtcgc
     5521 cggcggctcg atcacaccat gcgagcggga gagcgcgtcg cccggtgcgt tgggcgcgcc
     5581 gtcgccgggc tctgtgctcg atccggacga tcccgggggc gcggcaccgg gcgtgggcgt
     5641 cgcttggtcc ggaggtgccg gggtcgagct ttgtgccgcc gcggcaacgc caccgagcgc
     5701 cagcagggtg acgagtaaca acatcgaggc cggggccctc atggattgct ccgtgtcagg
     5761 gtcgatcatc ccgtcaacgg cccgggcccc cgaccgggtt cccccgcgcc gccgcgattg
     5821 cgcgccacac gcgctccggc gttgccggca tgtcgatgtg gtcgacgcca caggcgtaca
     5881 gcgcatcgac caccgcgttc atcacggccg gcagcgagcc ggcgcagccg gcttcgccac
     5941 agcctttgac acccagcaca ttcgtcttcg ccggcaccgg atggctcgcg aaacggaaca
     6001 ttggcgcgtc attggcgcgg ggcattgcat aatcgacgta gctgcccgtc agcagctgcc
     6061 ctgccgcgtc atggacggtg cgctcgagca gcgcctggcc gatcccctgc acgatgccgc
     6121 catgggcctg gccttccacc aacggcgggt tgatgagcgt gccgaagtcg ttgaccatcg
     6181 catagctgac gacggcgacc gcgccggtct cgggatcgac ttccacctcg gcaatgtggc
     6241 agccattggg aaaggaggac ggcggattgt cggagacatg ggcgacatcg agcgcgcgcg
     6301 gcaactccgg cggcggttgc agcccggcgc ggagttgcgc ggcgagctcc atgaggccga
     6361 ccgagcgatc ggtgccggcg attgcgaagc gtccgcgggc aaaaacgata tcggccgtgg
     6421 ccgcttcgag cacgaagccg gcgatctcct tgccgcgctc gatgacctgc gcgctcgcct
     6481 cgacgagcgc ggtaccgctc gccatcatgc tgcgcgagcc gccggtgccg ccgccggccg
     6541 ccagcgcgtc gctgtcgccc tgcaagaggt ggatctgctc gaaggggatg ccgagccggt
     6601 ccaccagcac ctgcgccagc ggcgaggcat ggccctgtcc gtagtcgagg gtgccggtga
     6661 cgatggtgac gctgccatcg gcctcgaaac gaatgccgcc cagctccttc gacggcggcg
     6721 ccgtcacttc gagatagctg ccgatgccca ggccgcgcag cttgccgcgc gccaggctct
     6781 gcgcgcggcg cgcggcgaag ccgttccagt cggcgagcga cagcgcctcg tcgagcaccg
     6841 cagtgaactc gccgctgtca tagctcatgc cggaagcggc cttgaacggc atctcgtcgg
     6901 cgccgatatg gttgcgccgg cgcagctcga tgcggtcgat gcccatctcg acagccgcgc
     6961 ggtcgataag ccgctccatg tagtaattgc cctccgggcg cccggcgccg cgataggcgc
     7021 cggtcggggt cgtgttggtg aacacgccct tggtcgacac gtcgaggagc ggcacgcgat
     7081 agacgctgac gacgttcttg acggcattga cggtgtaggg aagcaccgcc accacgaacg
     7141 cgccggcatt gccatagccg gtgaggcgaa gcgcaaggaa atggccggtg gcatcgagcg
     7201 ccagctcggc attcacaatg tgatcgcgcc cgtggtggtc ggaaagaaag ctctcggaac
     7261 gctcgtcggt ccatttgacg ggtcggccca gcgcgcgagc ggcgtgaagc aggcagacat
     7321 attcgggaaa gaccgacgcc ttcatgccga aggagccgcc gacattgccg gtgaggacgc
     7381 gcacgcgctc gggcggcacg gcgagcacgt cacgcgccag cgtgttgcgc agcccgaaca
     7441 cgccttggca gccgaggtga agggtccagc ggtctgcggc ggcggcatac tgggcgacgg
     7501 cggagcgcgg ctcgagcgga ttgacgacga tccggttgtt ggtcagcgtg agccgcgtcc
     7561 ggtgggcggc gcgggcgaag gcggcgtcga ccgcggcggt gtcgccgtag tggtaatcga
     7621 ccgcgacatt accgggcgct tcgggatgga gctgcggtgc gccgggcgcc acagccgctt
     7681 ccggcgtggt gacgacgggc aagggatcga tctcgacagc gaccgcctcg gcggcgtcct
     7741 tcgcttgagc tggggtggat gcgaccacga aggcgacggc ctcgccggca aagcgcacgc
     7801 gctcgtccgg aagggcgggc cgcggcggcg ggcgcatggc cgagccgtcg cggttgggaa
     7861 cgagaattcc gggcttgaga gcgccatagg cgccgagatc ggccgccgtg tagaccgcca
     7921 gcacgccggg catcgcccgc gcgttcgccc cgtcaacgcg gcggatgagg ccatgcgctt
     7981 cgcggctccg caccatcacg gcgtaagctt ggccgggaag attgacgtcg tccgcatagc
     8041 ggccttcgcc gcgcagcagc ttcggatcct cctgtcgcgg caccggttgg ccgatgccga
     8101 atctggtcag cgccagagcg gcgccgtcgc tcgcgtcatc catcatcatc cccgcgccag
     8161 gcggcgtgag agtaggggca gggcacgtcg gacggcaacg ggccgagcgc gcggatcgag
     8221 gctcgagcag gaatggaaca cggagttgtg gggagggccg ccggaaaccg tcgctcgctc
     8281 cggccgttag acaaggagca ggagcgccgc ggacggcccc ggctccggca caacttcaag
     8341 ggagcagccg atggatctct atcagctcat caagcaggat catcagaagg tcaggcggct
     8401 gttcgagagg ctggccgagg ccgatagcgg cacgccttcg caagcgcggc ttttcgccga
     8461 actgaagcgt gaactcgatc ttcattcaac cgccgaggag aagtatttct atccggctct
     8521 ccagcaatat gacgaggcca agaacctgat cgaggaagcg atcgaagagc atggcgacgt
     8581 caaggcgacg ctcgaggagc tcgatcactc ggacaaggag gacgagcgct gggccgagga
     8641 gctgtcggag ctccagcagg acgtcgagca tcatatcgag gaagaggaaa acatcatctt
     8701 tccgatcgcc gccgagctgc tcgacgcgga gcaggccgac gccatcgccc gcgacatcca
     8761 aaaggagaaa gctgcggccc agggaaccgc gtagccccgc cgccaatgcc cggcgggacg
     8821 agcgggcggc acggaggcgc ccgcccgcgg tggcggcggc acgcgatggc tcggcgaggg
     8881 ttggcgcccg taggcgccaa ccaaccgtta actattctca actctaatgt tcgggtacag
     8941 gcaggttctg cgcccgaaaa tgaaacgccc gcgactcaat ctgtcgccag cgaggatcta
     9001 tccgctgctg gactggttca tcccgccggc cgtcaaggcc gatcgcgaga tgctgcagcc
     9061 cgcgcgcatg ttcctgatca gccacctttt tggcccgttc atggccaaca cgatcgtcat
     9121 ctacctctat gtcatcgatc caaagcccgg cttcgggctg gccgtgctgg cgctcgcgct
     9181 caacgctttc tgggcctttc cgttcgccct caagtatctc ggccattatc agctgctcgc
     9241 gatgctgtcc gtcgaggacc tgatcttctc gatcttctgg ggctcctatc actacggcgg
     9301 cgtgaactcg ccgttcctcc cctggctcct ggtcgtgccc ctgctcgcct tcttctatct
     9361 cggtccggcg acccatctgc gccttcccgc cttgctgctg atcgccatca atttcggctt
     9421 cttctacctg tcctatgcgc tcggccattc ctttccggag catgtgcccc tgggcgcgct
     9481 caccggcatc ggcctcattt cgacgctctg ctcggccgtc tatgtctcga tgatggcgct
     9541 gtcctatgcg aacaccgtcg ccaaccagtc ggagctcgag cgcgaggcgc agaaccatcg
     9601 cgccaccgcc atcaagctcc gcgaggccaa ggaagaggcg gaagcggcca accgcgccaa
     9661 gtcagaattc ctggccaaga tgagccatga gctgcgcacg ccgctgaatg cggtcatcgg
     9721 ctacagcgag atgctgctcg aagatgcgga gatcgaaggc agcgaggagc agcaggtcga
     9781 ggacctgcgc aagatcaacg gcgctggcaa gcatctgctg tcgctcgtca ccgacgtgct
     9841 cgatctgtcg aagatcgagg cgggcaagat ggagctcttc aacgagcgtt tcgatctcga
     9901 cgggctcgtc gacgacgttg cggcgacctg ccagccgctc tgcaccaaga acggcaatga
     9961 gctcgtggtc gagcgcaaag gcgagctcgg catggccgaa ggcgacgtga cgaagctgcg
    10021 ccaggccgtc ctcaatattc tgagcaatgc cgcgaagttc acccggaacg gacgggtgac
    10081 gctgaccgtg gcgcgcgagc ccagggtcgg cggcgcctgg atccgcatcg acgtgcgcga
    10141 caccggaatc ggcatcagcc ccgaaaatct cggcaagctc ttccagaact tcaatcaggc
    10201 ggaagcgtcg acggcgtccc gatatggcgg caccggcctc gggctctcct tgacccagaa
    10261 gctgtgccgg ctcatgggcg gcgatgtgat ggtcgagagc cagctcggcc agggttcgtg
    10321 cttcaccatc cgcctcccgg gctcgctcgt cgactccgtg gagcgccgcg ccgatctcga
    10381 ctggacgacc gaggccatac gcgccgacgc cgccggccgg cagaataccg tgctggtgat
    10441 cgatgacgat ccggcgatgc tggacctgat gcgccgcatc ctgacccgcg aagggttccg
    10501 gccggtcctc tccgacgatc cgcgcaagac gctgagcctg gcgcattcgg cgaaaccgtc
    10561 gctgatcttc ctcgatgtcc tcatggcggg cgcgaatggc tgggaagtgc tgcgggcgct
    10621 caaggcggac gcggagctcg aggcttgtcc ggtggtgatg ctgaccatcg tcgacgaccg
    10681 acgcaccgcg ctggcgctgg gcgcggccga acacctgctg aaacccatcg accgcgacgt
    10741 tctaacccgc gtgctcgacc gcttctgtcc gcgtgacagg gccgctcgta ccccgaccgg
    10801 cgatgacaac caatcggtgg ccgccaatgc ctaagatact gcttgtcgaa gacaacgaga
    10861 tgaaccgcga tatgctcggc cggcgcctgc agcgccacgg cttcacgctc tgcttcgcgg
    10921 ccgacggacc gaccggcatt acgatggccg gtacggagat gcccgatctc atcctgatgg
    10981 atatcgcgct tggcgagatg gacgggtggg aagcgacgcg gcgcatcaag gcgaacccgc
    11041 caacctcgcg cattccgatc attgcgctca cggcccatgc gctggcgagc gatcgtgaca
    11101 agagtgtcga ggtgggctgc gccgacttcg acaccaagcc ggtcgacctg ccgcgcctcc
    11161 tcgccaagat caatacctgc ctggcgaacg gcaagactca ataacgagcg gggaaaacgc
    11221 ctcgcgcgcc gcgaggcgga cggtcaggaa tcctggcgca gccggctcaa gagctggcgc
    11281 agcgcgttga tgtccaccgg cttctgcaga tcgtcgacga ccttgagccc gcgcacttcg
    11341 gcgagccggc tcgcggcttt gcggaaccag ccgtcctgac cgctgatgat gaccagaagg
    11401 cctgcgaagc ccgtctcctc gagcatggag aggaggtcga tgccgtcggc ctcgggcatg
    11461 cacaggtcga gcgtcagcac ctccggcttc cagctcacca ccagagcccg caccgatttc
    11521 ggatccgaga tggcctgggc gtcgtaaccg catttcacgg cgacgcgggc gacgagatcg
    11581 gccgagtcgg cactgtcgtc gacagcgagt aggcggcctc tgttggtgtt tgcctgcatg
    11641 gctcttagtc tcctgctgaa gcgacggcgc ccttggctgc cagttgcgga taggagcgca
    11701 gcgcgccctg cgcctcatca taggccactc ggaggcggct gaccgcctgg tcgacgttgt
    11761 ttctgctgtg cttcgccatc acctggaagc ggaagcgcga gctgttcttt gccacggccg
    11821 gaaattcgac catgttggcg aggacgccga ggctcggcag ccggcgggcg accagacgtg
    11881 cgaggctctc ggagcgcatc atcgccggca cgatggccga cggctcgccg agcacctcga
    11941 agccggcgtg ctgcagcgat tgccgcagat attcgacgtt ggtcatcagc tgcgtgcgca
    12001 aggcccgccc ctcagccgac ttcacgatct cgaaggcctt caacacgatc cccgactgga
    12061 tcggcgagag ggcattcgag aaggtgtgcg gcgcgctgta gaatctcaga tattccctga
    12121 cggcgcgctg gttgcaggcg acgaagccgc cattcgaggc gaaggtcttg gagaagctcc
    12181 ccatgacgat gtcgacaaga cccagcatat gctgcaagcc gatatggccc ttgccgtcat
    12241 cgccgatgtt gccaaggtca tgagcgacgt cgaccaggag ggtggccccg aactcacggc
    12301 agagatcctg cagcgccgcc atgtccggcg tgtcggaatc catggagaac aggctctccg
    12361 tcaccaccag gatcgcgttc ttcgtgtcgc gggcgcggat ccggctgagg taccggctca
    12421 ccgcaagcac gttcaggtgc ggatggagat gcacgttgcg ggtcgccgcc gtcgcgcctt
    12481 ccttgaggca ggaatgcgaa agggcgtcca tcaccacgtg atcgtcgggg cgcaccagcg
    12541 ccttgatgac gccgaacccg gccgcccaac ctgtcgggta gaggaggacg tgctccatgt
    12601 ggaggaaatc cgccagcacc cgctcgagcg caagcgaggt ccgcgtattg ccgagaagcg
    12661 ccgcggttcc cgcgctgtgg accccatatt ggtcgatcgc cgctttggcg gtctcgtgga
    12721 ccgccggatg cgaggcgagg ctcaggtaat cctgcgctgc gaaattgacg ccggtgaagc
    12781 gctcgccgct ttcatcccgc gcggcgcaga ccggggctac cgcctcatcc gtcgatcgcg
    12841 aatagggcca gaggtcatgc tgttgccgca gcgcctccca gcggaaaaac tgttcgaccc
    12901 ggcccaaaag gtcggggccg tgcggctcgc ggaagtcgcg catgctaccg gtcaaggctt
    12961 cgatcggtac agtgctgtcc gtcatgtcgg tgccgcgccg cccttcgcac aagcccgctc
    13021 ccccgcgtcg ttccgagttg cgaggggcct gatatttgcg aagtctagca aagggtcgtt
    13081 aaaatagcgt aaaagatgga gatttgatta agttacggcc acccatcccc ggtctttgtt
    13141 gggggtacta cttaacccgg gacccctaat acagcgagtg ttacttcagg ttactcgccc
    13201 tgggtgtccg cggtaatgcc ggaggctcgt gcgcgctcct cgggcagcga cttcggcagg
    13261 cggatggtga cggcggtccc ctggcgcggc tgactgacga tggcgagcgt gccgcccagc
    13321 atttcgacat attggcgcgt cagcggcata ccgaatcccg ccgtgccctt cggctttccg
    13381 gaggcggtgc gcagctggtc gagcggccgc agcatctccg gcagctcctt ggccgcgatg
    13441 ccgattcccg tgtcgcggac aacgatgata taggcgccgc cgccagcatc ggggccgact
    13501 tcgaccgtga cggtgccgcc cgcgggcgtg aacttgacgg cgttggacgt catgttgacg
    13561 acgatctgca tgagcttcgc gcgatcggac tgaacctcct cgacgcccgg cttgaccacg
    13621 accttgagcg cgacgccggc cttctcggcc tgggccgaga tcagcttcac gctctcgttg
    13681 acgacgctgc cgacatcgac ggccgatacc gcgaggggct cttgccgcgc ctcggccttg
    13741 gccaggtcga ggaagccgtt gaggacctgc agcacgttca gcccggcgcg gtggatgtcc
    13801 tcggcatatt cgcggtagcg cggcggcagc gggccgaatt cctggtgacg catcatgtcg
    13861 gagaagccgc acacggcgct catcggcgtg cgcagctcat gggcgagctc ggccaggatc
    13921 tccgcccgga tgtgggcgtc ggcttcggag cgcaccagcg agcgcttgag ctccgactcg
    13981 gcgccagtga gcacgtcgag cgcctgctgc aggcgctggg tgcgggcatt gaccgcctct
    14041 tgcaggcgct ggttctggtc gcgcatctgc cgctgaagcg cgcgcatctc ggcggcgccg
    14101 gcgacgcgcg ccaccagcac cggggcctcg aagggcttgg tcacgaagtc gctggcgcca
    14161 cgctggagcg cggccacctg cacatcgtgg tcggcttctc cggtcagcat gatgatcgag
    14221 cagccgaccc ggtcggcgtc ggcgcgcagc atgtcgagca cggcgaagcc gtccatcccg
    14281 ggcatgtaga ggtcgagcag cacgacatgc ggcttgacct cgcgcagaag cgaaagcgcg
    14341 atcgacggct cgctggtcga atcgacggtg tggaacccgg cgcgggttaa caccctcttc
    14401 accaactcga cgctgaccgg atcgtcgtca atgacgagaa tgcggctcga tcgtgcggtc
    14461 tcgctgatca tgcggggtgc atgataacaa atttcttgtt cgcaaatgct taatggtcgg
    14521 cgccgctaat agggtgtgcc ccgtgcggca ggagaaagaa tcatgatgaa tacccccgcg
    14581 ctccaggaca agcgggtcct gattgtcgac gacgatgaac cgacgcgcag cctcgtccga
    14641 cgtgtcctga agcgcatgaa gatcgccaat ctgcaagaag ccgccggtgc cgagcaggcc
    14701 atcgcctgcc tcaacgagtc ctccgaagac atcgatctcg tcatctgcga ttggaatatg
    14761 ccggggatgt ccggcatcga attgttcgat cgcgttcatg cgttgaggcc cgagcttccg
    14821 ttcctcatgc tgaccgggcg cggcgacgcc acgtcggtga tcgccgcgaa gaaagcaggg
    14881 atccccgctt atatcgtcaa gccgatcttt ccgcaggagc tgaagacgaa ggtctcgttc
    14941 ctgctgatga aggacgcctg atcgccggtc cggcgatcag aaggccgcgg cggcagcggt
    15001 cgagaagagc ggcgccgcgg cgccgtcgac gctcgcgagg cgcggcggcg gcgatatcac
    15061 cgcgggaacg cggatcgtga aggacgagcc gcggcccaag tcgctctcga cggtgatctc
    15121 gccacccatc atgcggcaga gcttctggct gagcgccaga ccgaggcccg tgccgccgta
    15181 gcggctcgac gtcgacgcct cggcctggtt gaagttctgg aacagtttcg gcaggttctc
    15241 gtggctgatg ccgatgccgg tgtcgtgcac ggcgaggctg atccagtcgc cctgcggcgt
    15301 ccgctcgcgc atggcccgca gcacgacgcg gccgccattg gtgaacttgg cggcgttgct
    15361 gaggagattg agcgccgact ggcgcagctt ggtggcgtcg ccgatgatga cgccgagagc
    15421 gccctcgcgc tcgacgacga aatcgttgcc gttctccatc accagcgggc ggcatgtcgc
    15481 cacgacgtcg tcgatgaagg tattgaggtc gaaagactgc accgacaggt ccatcttgcc
    15541 ggcctcgatc ttcgagagat cgagcacgtc ggtgaccagc gacaggagat gcttgcccgc
    15601 gctgttgatg cggcgcagat cagtgatctg ctgctgcttg cggccgtcgg tctccgcgtc
    15661 ctcgagcagc atctcgctgt agccgatgac ggcgttgagc ggcgtgcgga gctcatgact
    15721 catcttggca aggaactccg acttcgcccg gctcgcggcc tcggcttcct ccttcgcctc
    15781 atgcagctgg atcgcggtgg cgcggtgctt ttgcacctcc tgctcgaggg acgattgcga
    15841 ggccacgaca ttggcgtagt agatcgccat catcgacata tagacggtgg cgcagaggac
    15901 cgaggaaagc ccgagccccg acatggccgc aatcggcaca tgctctggga acgacttgtt
    15961 cacggcgtag gcgagataga agcccacgca gttgagcgcg aagagcgcca gcaccagata
    16021 gcgcagcttc ggccggtcgc cgagatagaa gaagagcagg agcagcgcat tgagcagcca
    16081 gggcaggagg ggcgagctca cgccgccata atgataggag ccgaagagcg tgacgaaggt
    16141 caggttctcg accgtgaaga gggcgagcgg caggaggcgg ccggtgacct tgagccagaa
    16201 cggcagcgtc cagaacgacg tgatcgagcc gacgatcgcc cagtaggcca tacccggatg
    16261 cgggtcgagg atatagaggt agatgctgat gaactggccg agaaagggac cgaagagatg
    16321 gctgaacacg aagatgcgcg cgcgctcgag cgtcgcggcg tcggtcctga gtgtctccgg
    16381 aatgaaccag tcggacttgc gatagatgac cgcgccgatg gccgagagcc gcttcagcat
    16441 gagccaggcc tcccattccc cggcggttca tggcttggcg gcagtctatc ggctactggt
    16501 taacggttgt tttacgtttc aggcacggcc gccgtaggat cgattatcgt gccggccgcg
    16561 agatcctgca tcggcctccc gcccacggca taggtgaggc atccgcgcaa atgctgaaac
    16621 ccggcgcggc gatagaatgg gcgcgccggg ttgtcgtctt gcacattgag ctggatgagc
    16681 gggatgcgcc gctccaccgc gtgcgcggcc agcgtcgcca gcagggcgcg ccccgcgccc
    16741 gagcggcggt gagccggctc gacgaagaga tcctggatcg agagcaccac gtccgccagc
    16801 gccgtcagat agaattcgct gtaggtgagc ataccgacaa tggtgccgcc gctctcggcg
    16861 accaaagacg caaatcgcgc ctgggtgccg aagccgtccc gcagccaatc ggccgccgtc
    16921 gcccgaagca cggcttcgtt cccctcggcc ttggtcagcg cctgcttcag gcggaacagg
    16981 gccgggacgt cggcgggcgc tgccggtcgg atgcgcaggg cgggaggccg cagatcatac
    17041 tccatggtgg ctcgacacga gcgtgcgctc gaccgctttg ttagcaaatc atggttagcg
    17101 acaggtaaaa ccgcggattt tggcaaaaac ttaacgatcg ccggctcacc ttggctgaac
    17161 gcatcgagcg agcgagacca atgtccgcag agcgccagat tccggccaaa ccacccaaag
    17221 ccccggccgt cacgccggcc gccggtgcgc cgccgatgct cggcaacgaa gagatgttgc
    17281 tcgcgtatgt cgagcagctc gccgatgccc gcaagggtca gcgcgcgatg gtcgtccatc
    17341 tttcgcggct gcagggcgcc aagccacgcg acaagaatct gcagatcgtc gccgccgtgc
    17401 tgcgcgaggt gacggaccaa ttctccgggc ggcttttcgc gctggagaac ggcgacatcg
    17461 ttgtcgtctg caaaggcatc acccgccggg cgatcgacga gacgatcgag gtgcttcgct
    17521 atctcttcaa cgacgatccg gtgacacgcg ccggaaccga gcaagccgat ttctgcgcca
    17581 tgttcgatct cgaagtcggc tatgtcgact ttatgtttgc gctcgacgag attcgcgagg
    17641 cggcgcggcg ttcctcgatc acggcagggc agcgccgccg cttcgatccg ggcgcgatcg
    17701 atccgccgca cgccggcgat ctgttcaagg cgctgcaacg cgtcgagctc gccggcatgc
    17761 tgcgccgcca gacggtctgg gacatggcgc aagatacggt gccgcggccg ttcttcgagg
    17821 aaatcttcgt ctcgatggac cggctgcaaa aggcaatcgg cccggcattc gatctcgcca
    17881 aggaccggca gctctttctc tatgtgagcc gctggctcga ccagcatgtg atggcgacgg
    17941 tcgcccgtca ccaaagcaat gtttcgctgc ggctgggctt cagtctcggc cttgcgacct
    18001 tgatgtcgcc ggaattcgac gatttcgaca agcgcggcgc gatcggcccc gagcgcgccg
    18061 tcctggagat accgcttgcc gacctgttgc gggcgccgtc aacctttcgt caggtcgcgg
    18121 atcgcctcaa gcagcggggc taccggctgt gcatcgacga tgtcagccac cagatccttc
    18181 cttatgtcaa tctgcgtccg ctcgcgcccg actatgtgaa agtgatctgg gacgatgcgc
    18241 tgatgcatct cggcgaggag cccttgcgcg aattctgcgc ggcgatgacg gcggtcggca
    18301 gcgagcgtat catcctctcc cgttgcggtc ggcgccaggc gatccgcgtc ggccacgcca
    18361 tgggcatccg gttgttccag ggctggcaga taaatcaagc caatcgatag ggttgaattc
    18421 cccgcgaaag cgcggcatct ctccacggct ggcgcgaagc cgcccatggg agtgtcgtcg
    18481 tatgaagttc gggatcggtc agcccttgcg gcgccatgaa gatctgcggc tgctgacggg
    18541 aaagggccgc tataccgacg acattgctct tccgggcatg gtttttgctg cgatcttgcg
    18601 ctcgcccgtg gctcatgcgc gcctcttcaa ggtcgatgcc accgccgcgc gcggcatgcc
    18661 gggcgtcctg ctcgtcctca cgggcgtgga ggtgacggcg gatcagctcg gcaatctgcc
    18721 ttgcgtcgct gcgatcacga gccgcgacgg ccggccgcgt catgacacgc cgcggccgat
    18781 cctggcgatc ggcaagctgc gccatgtcgg gcaggcgatc gccatcgtgg tcgcggagac
    18841 gctccatcaa gcgcgcgacg ccgccgaggc catcgaggtc gactatgacg agctgccgcc
    18901 cgtcgtcgat gttcgcgcgg cgacggcgcc gggcgcgccg cagctcttcg accagatccc
    18961 cgacaacctg gtgttcgatt gggacaacga catgacggat gcggcgcgga ccgacgccgc
    19021 ctttgccaag gccgcgcgcg tcatcgcgct cgagctggtc aacaatcgcg tcgtcgcgaa
    19081 ctcgatggag ccgcgcaacg ctatcgccga ctacgattcc cagagcgatc ggtcgacgct
    19141 ctataccacg acacaaggcc cgcatttcgt gcgcgatccg ctcgccatgg cggtcttcaa
    19201 gttcgccaaa gagaaactcc gcgtcgtgac gcccaatgtc ggcggcgctt tcggcatgaa
    19261 ggccttcgtc tatcccgagc aggcgctggt gatctgggcg agccgcaagc tgaagcggcc
    19321 ggtgaaatgg caggccgatc ggtccgaggg cttcgtctcc gacaatcacg gtcgcgatca
    19381 tgtcacgcgc gccgagctcg cagtcgacga caaaggccga ttcctcggct tgcgcgtttc
    19441 cacgctcgcc aatctcggcg cctatctgtc gccgctcggc ccgttcattc cgacgcgctc
    19501 ttccgatctg gtcgccgggc tctacacgac tccggcgatt cacatcaatg tgaagggcgt
    19561 ctgcaccaac acgctgccgg tctgcgccta tcgcggcgcc ggccggcccg aagcggcgta
    19621 tctgatggag cgccttgtcg acgtcgcggc gcgcgatctc ggcatgacgc ccgatgcgct
    19681 gcgccggatc aacttcatcc cggcctccgc catgccttat acctcggcga cgaaaatcgt
    19741 cttcgattcc ggcgaattcg aaaaagtgat ggatttggcg atgaacgccg ccgattggac
    19801 ggggttcgct tcgcggcgca ccgagaccga acggcgcggg cggctgcgcg gccgcggcat
    19861 ggcgacctat accgagcgct gcggcggcgg ctttcccgag gcggcgtcca tcgagttcaa
    19921 ggacagccgc gtcgagctcg tcatgggcaa tatcgaatac ggcacgggtc tcgtcaccgc
    19981 ctacaagcag atcgtcgccg actggctggg cctcgacatc gatcgcatcg aggtcgtgat
    20041 gggcgatacc gatcgcacgc cgtcgggact caccggcggc tcgcgcgcgc tcgccgtgag
    20101 cggctccgcg ctctatgagt gctcgcagga gatcatcgag aaaggccgca tgctggcggc
    20161 ccattttctg gagaccgcgc ccgccgatgt gcagtttgcg gaagggaact tcttcgtgcc
    20221 cgggaccgat ctgcgcctcg atctgcttga cgtcgccacg gcggcacgcg atccaacgaa
    20281 gctgccgccg ggaatgacac ccggactcga cacgacgaag cagcggacac cgctggccaa
    20341 cacttttccc aatggctgcc acatctgcga gctggagatc gaccccgaga caggcgtggt
    20401 ggcgatcgac cgctatacca tcgtcgacga tttcgggcgg accatcaatc cgctgatgct
    20461 tgagggtcag gtccatggcg gcgtcgtcca gggcatcggc caggcgttgc tggagcacac
    20521 cgtctacgat ccggacagcg gacagctcct gtcgggctcc ttcatggact acgccatgcc
    20581 gcgcgccgac gatgtgccgt ccttcgcgtt tgccacccac aatgtgccct gcacggcaaa
    20641 tcccctcggc gtcaaaggcg cgggcgaggc cggcgctgtc ggcgcgcccc cggccatcat
    20701 caatgccatc gtcgacgcgc tccatgcgaa gacgggattg cgccacctcg acatgccggc
    20761 gacgccgcgc cgggtgtggg aggcgttgca gcgcgcgaag tgaagctggc ggcgccggct
    20821 actccgccgc cttttcacaa gcaactggcg cccgtccgag ggacatggtg tagtcgccgc
    20881 catggaggtg ctcatcatcg gcgcgggtat cggtgggctc acgctcgcct tgatgctgca
    20941 ccgcgccggc attccctgcc gcgtcttcga agccgcggcc gagatcaagc cgctgggcgt
    21001 cggcatcaat cttctgccgc atgccatgaa ggagctggcc ggtctcgggc tcgaggacac
    21061 gctcgcgtca cgcggcgtgc tgacggcgga agccgtattc ttcaaccgct tcggtcagct
    21121 catctaccgc gaaccgctcg gccgcgccgc tggctacgct tggccgcaga tctcgatcca
    21181 ccgcggcgat ctccaggcgg cgctggtcga ggccttcatt cagcgcctcg gcaaagaccg
    21241 cctgatgctc ggctgggaat gtgtgagcgt cgcttcggag gaaggggcgg ctgttgccca
    21301 cttccgcgac ccgatcgcaa agcgggaatt gccggcgcag cgcggcatcg ccgccatcgg
    21361 ctcggacggt atccactccg ccattcgccg ccagctttac cccgacgaag gcgggccgat
    21421 ctattccggc gtcaacatgt ggcgcggcgt gacgcgctgg ccgccgatcc tctcgggggc
    21481 cagcatgatc cgcgccggct ggttcgcgca cgccaagatc gtcctctatc cgatccgcca
    21541 tgatgtcgat gccgagggtc ggcagctcgt caactgggtg gtcgagatcg ccacgccgag
    21601 atatgagggt tggaactgga gccggccggc gacgctcgac gatttcatcg cgccgatgac
    21661 agactggcat ttcgactggc tcgacgtgcc ggaatttctt cgccgctccg acctgatgct
    21721 ggaatatccg atggtcgaca aggatccgct gccgcgctgg agcttcgggc gcgtgacatt
    21781 gctgggcgat gcggcgcatc cgatgtatcc gcggggatcg aacggcgccg gccaggcgat
    21841 tctcgatgcg cgtgcactgg ccgactgtct cgccacgtcg agcgacgccg tggccgcgct
    21901 cgccgcttat gacgagcggc ggcgcgacgc cacggcgcgg gtcgtccgtg ccaatcgcgc
    21961 ggcaccaccc gatgccatcc tcggcgaggt ctaccggcgc accggagaca agcccttcgc
    22021 ctcgattgac gacgtcatca gccgggagga gctggctgcc atcaccgaag cctataagcg
    22081 cgtcgccggc tatgagaagc aaaaactgag tggcgaagac tgactactgg ttcgagacac
    22141 atatcaccag gtcgagcgcg gggctatcgc atatgggttc accacggagg cacggaggac
    22201 acggagaaga ggggttcggc gcgcgaagcg cgccatcctt cgcttctcgg tgctctccgt
    22261 gcctccgtgg tcaatctttc ccatcgccgt ctcggcaaga tatgcgattg ccctgaggtc
    22321 gagggcaggg tttgctcgat gacattggcc gtgaagaacc gcgaaaatgg ctgttgcccg
    22381 gctcgcgtca aaaatcgcaa gatatgccag cacagccctg cgtatccgcg agtcgccgcc
    22441 ggatgaacat gtcgacgccc gagcaggtat tccgcaccgc gctttccttg cagttccgcg
    22501 gccgattgag tgaagcagag cgtctttatc gaacgctgct gaagagcgtc cccgatcacc
    22561 tcgattgctt gcagaatctg ggcaacgttt gcatcgagca ggggaaactc gaagaagcgc
    22621 gccgattttt ccagaaggca ctcaatctga aacccgggtc ggccgacgca cgcaacggcc
    22681 tcggcaccgt gctgcaggcg ctgggtcgcg accaggaagc agccgtccag ttcgagaagg
    22741 ccattgccgc cgagccgaca ttcgtcgagg cacgcaacaa tctcggaaac gtgttgcagc
    22801 gtctgcgccg tcatgacgag gcgatcgcgc aatatcgcca agcactcgcc ttgcgtcccg
    22861 aatatccgtt ggcgcgcgcc aatctcgccg atgcgctcgc ggcgctgcag cgtgacggag
    22921 aggccgtcga ggaatacgaa gaggcgctgg cgctgcgtcc cgatgatccg gcgatccact
    22981 ataacctcgg gaatgctctc cagaagctgg atcggcccgc ggatgccgtc cgccactatg
    23041 agcgagcgat cgccctcaag cccgacgatg ctcacgccca tacaaatctc ggcgccgcgc
    23101 tccaagcatt gaaccgtcat ggcgaggcca tcggccagta tcaaaaagcg ctcgccctga
    23161 ctccggacga cgccaacgtc ttgtggggcc tgggcctcgc cgagctcgct gtcggagatt
    23221 acgaaggcgg ctggcggcac cacgaatgca gatgggatgc gccggcgaca ggccttcggc
    23281 cgcgaaattt tccggcaccg gcctggcagg gcaaggagcg cctcgccggg cgcaccatcc
    23341 ttctccatag cgaccaaggc ttcggcgaca ccatccaatt ctcgcgctac gtcccattgg
    23401 tcgccgcgga gggtgccaag gtggtgctgg aggtccagag acccctggtg cctctgctgg
    23461 cgtcgctgca agggttgtcc gagctccgtg ctcagggcga agcgctgccg gcattcgatc
    23521 tccattgctc cctccagagc ttgccttttg cgctcggtac ccgactcgag accattcccg
    23581 ccggcgtgcc ttatctcaat cctcccgcag acgccgtcgc acgatggcgg tcgcgattgc
    23641 gaccgcccga cggctcgccc cgcatcggca tcgcatgggc cggcaatccc gcgcagcgca
    23701 acgatcgcaa tcgctcgatt ccgctggcgc ttctccgccc gcttctggcc aagacaccgg
    23761 gaatcgtcgt ccttcaacag gagcttcgcg atggcgatgg cgaaatcctc ggtgcgctga
    23821 gcacgatcgc gctgcatgag gggcgttttg ccgacttcgc cgagacggcg gcgctgatct
    23881 cggcgctcga tctcgtcatt accgtcgata gtgccgtggc gcatctctcc ggcgcgctcg
    23941 gcaaaatgtt gtgggtgctt ctgccttatt cgacggactg gcgctggccg gtcgaccgca
    24001 cggacaatcc ctggtatcca accgcacgtc tcttccgtca gccgtccatc ggcgattggg
    24061 cgagtgtgat tcgatccgtc gccgccgcgc tcgatcgaca gacccgcgga ccatcttcgg
    24121 cgccctgacg ctgacggact gctggctcat gaggttcttt gaccggcacc ttcgcagttg
    24181 cggcaaagct cttgaaaatc cggattttcg aacttagttg acgcgcgccg tccgcaggcc
    24241 aataaacgca gatacggggc tgcggcgcga gccgaggcag cagcgttcct catgaggagg
    24301 cgagcgcccg gatgcgtatt ctcgtggtgg aagacgatgc cgaggcggcc gcctatctgg
    24361 tcaaaggcct caccgagagc ggccacagcg tcgatcttgc cgccgagggc cgcagcgggc
    24421 tcgaccgtgc ccgacgcggc acctacgacg ccatgatcat cgatcgcatg ttgcccggtc
    24481 tggacgggct ctcgatcatt cacgccttgc gcgaggcgcg gaaccagacg ccggttctgg
    24541 tgctgagcgc gctgggcgag gtcgatgacc gggtcaaggg attgctggcg ggcggcgatg
    24601 attatgtcgt caagccattc gcactcaacg agctgctggc gcggctggcg gcgctgacgc
    24661 ggcggggtaa tgccgagacg cgcctcgtcg tcgccgatct cgagatggat ctgctctcgc
    24721 gcaaggtgac gcgggccggg cgcgaggtcg agctgctgcc tcgcgagctc aggctgctcg
    24781 aatatctgat gcggaacgcg ggacacgtgg tgacgcggac gatgatgctg gagaaagtct
    24841 gggacagcca cgtcgatccg cagactaacg tcatcgacgt ccatgtcggc aggctgcgtc
    24901 agaagatcga caagggattc gcgcggccct tgctccacac ggtgcgcggc gcgggttaca
    24961 ccctgcggcc cgagtgaccc cgtgctgcgg ctgctgcgga ccaatgcctt ccgcctcgcg
    25021 gcaggctatt tcgcgctttt cgcggcatcg gtgctggcgc tcctcgcctt cgtctatctc
    25081 tcgacagcgg acttcgtcga gaaacagacc gaggccacga tcaatgccga gattcgcggc
    25141 ctcgctacgc aatatgagca gaacggcctg cacgggctga tcgatctcat caacgcccgc
    25201 atcgccggcc agcagatacg cgcgacgctc tatctcatca ccgatgaccg gctcaacccg
    25261 attgccggca atctctccgc ctggccggca gcgacgacgg tgcgcccggg ctggatcgag
    25321 tttccggtcg aagcgcgaga ccgggacgcg cgtgagaccg gctcggcgct cgcctcgatc
    25381 ttcgtcttgc gcgacggcta tcgcttgctc gtcgggcgcg atctccgcga cgtcacggcg
    25441 ttccgctcgc gcttcaatca caccttgggc tggaccgccc ttgccacggt ggcgctcggt
    25501 cttgcgggcg ggctgttctc gacgcgcaac atcatgcgtc gcgtcgaggc gatgaaccgc
    25561 acgagcgaga acatcattcg cggcgatttc agccagcgcg tcgcgctcga cggcagcggc
    25621 gacgagttcg atcagctctc cgccaatctc aatgccatcc tcgagcgcat cgagcggctg
    25681 atgaccggga tgcggcaggt gaccgagaat atcgcgcatg acctccgcac gccgcttgcg
    25741 cgcctccggg cgcggctcga gatcacgctg ctggagcggc ccgatgcgga ccgctcgctc
    25801 gaagcgctgc gcgagacgat cgccgaggcg gatcgcctgc tcgccacctt caacgcgctc
    25861 ctcaggatcg ccgaagccga agccggcgcg cggcgtcaca ccctggcgct ggtcgatctc
    25921 gccgagatcg cccgctcgat ggccgagctc tatgcacccg tggcggagga aaaggggttg
    25981 gcgttcaccg tcgaaacgga ccccgtcatc gtcgtccgcg gcgaccggct gctgctgtcg
    26041 cagatgatcg tcaatctcgt ggacaacgcc ctcaaatata cgtccgccgg tacggtcgcg
    26101 ctcacggcgc gccgccaggg aacgctggtc cgcatcgaga tcgcagatag cggcccgggg
    26161 attccggccg accgtcgcga ggtggtgttc gatcgcttcg tccgcctcga aggcagccgg
    26221 agcacgccgg gcaacggatt ggggctgagc ctggtgcgtg ccgtcgcgac gctgcatggc
    26281 ggcgatgtgt cgctcgatga cgcttatccc ggtcgcagcc cgcgcggcct caaggcggtc
    26341 ttcaccttgc cggcaagcga gggcagcatg gcaccgcgat tgcccccggt cgcgctgttg
    26401 cgcaaaccgg cctgagcact gcccgatacg tccgggcccc ggccatggcg gtcgctacgt
    26461 atcgtcggat tcggcggtgc ttcggctcgt cgagcggcgc agacgcttct ttgcgagcgc
    26521 cgtctggaag gcgttgagat gaggtttcag ggcagcgtgc gcctcgtttt ccatgtcgcg
    26581 gtagtggcgc acgaggcgct tgccgaatgc cgtgagctcg gcgccgcccc cggccgcgcc
    26641 gccaagccgc gtcgcgacga cgggacggct gaagagacgg ttcagatcgt cgatcagcag
    26701 ccaggcgcga cgatatgaca tgttcatcga ccggccggcc gccgagatcg agcccgtcgc
    26761 gtcgaccgac tcgaggagcc gaatcttgcc ggggccgacg gcgcccgagg gagcgaggtc
    26821 gacgcggatc gacagctttg ccattgccgc gatggtagca gcttcttccg gcaaaagaag
    26881 gctcgcgccc gctcggccgg cagcacgatg ggtgccaacg ccgggagcaa tgcctcctca
    26941 gaatcccaca tcggactggc cgggcaagac gatcccggtc tgcggatcga ccagaagccc
    27001 gtcgccgtcg cgagggccgt tgggatcggg ataggttccc ggcgccgaca cgcagccggc
    27061 aagcaggaga gcggtggcgc cgagcgccag caggacggtg cggtgaagca tgggttcctt
    27121 tgccatggtc ttgacctctg aaatgtaccc gcggcaagcc agttgcgccg gctcacatga
    27181 caggcatttt aggtgccgca ggagataatc cggtttagcc cggctatatt cccgccaccg
    27241 cgcgagcggc attccttgcc gtcggcacag gcctctgctt cagagtcccg gatcggcctg
    27301 gcccggcagc tccaatcccg tctggggatc gatcagaaaa ccgtcgccgt tgcgtgggcc
    27361 gtcgtagtcg ggataggtcc ccggcggcgc ggcgcagccg gcagccagaa gagccgtcag
    27421 cacgagcgcc aggagggcgg tactcggaag cgatgtggaa tcgtttgcca tagggcacct
    27481 cgagacaaca tgcgcgccgg cgtccacgaa acgacgcgtc gccgagacta gcgatatggt
    27541 cgaggcgaat gtccgcgcaa gtgccggctg ccgggctaaa cctcatttta gccggcgtcg
    27601 aatgacggcg accggcaagg tcgtcagagc ttcggccaaa gccccgacca ccagctctcg
    27661 gatttctgct ccgccggagc cggggcgtca gcggtttcga ccgcgtgctc gtccggcagg
    27721 tcctgaatct gccacccgcc gcccagcgtc cgcacgagat cgacgctcgc ctccagccgg
    27781 cgatattgga tgacgagaga ggtcagctcg gcctggagtg ccgcggtctg cgcgaccacg
    27841 acctcgaggt agttgacggc gcctccgcga tagaggctga gcgatagatt gagcgtttgc
    27901 tccgcggcct gcacggcggc cgcttcctcc tgcgcctcct gcgccagaat acgcagcaag
    27961 gcgagattgt cctcgacttc ctggaaagca tccagaacgg tctggcgata attggcggcc
    28021 gtctcattga aggcggccca agccgcctga tccgcggcat tgcgcagccc ggcgtcgaac
    28081 agcggcagcg tgacgctggg accgagcgac cagaagctga agggcgcggc gatgagcttt
    28141 gatccgccgg tattttgaaa gccgccgagc aggtccaggc tgacattggg atagaaggcg
    28201 gcgcgagcca ctccgatgag ggcgttggcc gcggcg
//
LOCUS       DASYNS010000441         3544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5572176, whole genome shotgun sequence.
ACCESSION   DASYNS010000441 DASYNS010000000
VERSION     DASYNS010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5572176"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VGP50_13800"
     CDS             <1..1192
                     /locus_tag="VGP50_13800"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV8028503.1"
                     /translation="ALRAVPADTLCYEELLAAERDEFAWPMFDERLASSLCYTSGTTG
                     DPKGALYQHRSTVLHSYGSALPDCLNLSARDVICPVVPMFHVNAWGLPYSSMLVGAKL
                     VLPGPALEGKSLYELFEAEQVTMSAGVPTVWFALLQYMSERGLRFSALQRLVIGGSAC
                     PPAMIERFEDDHGIEVVHAWGMTEMSPIGTFAQPKGKHRGADTAQLRVMKSKQGRVIP
                     GIDLKIVDGAGKELPWDGVSAGDLMARGWWVTSAYYQTEPGSALQDGWFPTGDVATID
                     SDGYMHITDRSKDVIKSGGEWISSIELENIAVGHPAIAEAAVVGVPHAKWGERPLLVA
                     QVKSGAKLTRDELLAFYRGKTAAWRIPSDVVFVDALPHTATGKLLKSELRRRYADYRL
                     PAET"
     gene            complement(1168..2499)
                     /locus_tag="VGP50_13805"
     CDS             complement(1168..2499)
                     /locus_tag="VGP50_13805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV8028504.1"
                     /translation="MIRPTRSPWFFVLLALALAGCNAASSGSAPDTAGPSASDLAFYR
                     SVLDKVHQSYVDPVTDSTLVDNSLKGMLTGLDPHSDFLTEREYQDMLDDSAGEFAGIG
                     AELTRDQDHPVVLSPIDDTPASHAGIRPGDAILRINGRITEGLSLKDVVDALRGPAES
                     KVQITIGRKNTAPFDVTLTRAIIHVDSVKSALEPDGMGYIRISSFAETTQREVTKALD
                     ELKTKSHGKLKGLVLDLRNNPGGLLDQAVHVSGDFLDGGLTVSIRGRSPDDNRPFPAP
                     ANGDRIPGVPMVVLINGASASAAEIVAGALQDRHRADVLGTQSFGKGSVQTIIPLEGH
                     GALRLTTARYYTPSGRSIQGQGVTPDRIVEQPKGDQAADVDLVREADLPGALDTGAKA
                     ASKTAAAPGLPAPVKSETLIEAAAIGTAKDVQLKAALEDLDKKFQVSAGNR"
     gene            complement(2603..2944)
                     /locus_tag="VGP50_13810"
     CDS             complement(2603..2944)
                     /locus_tag="VGP50_13810"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEV8028505.1"
                     /translation="MAIRGQQSEPMELHPGAGVREAARVAELEKWAWGRARSLKIFYS
                     HLAAYVIINFVLFLIDSTSPGPAWFYAPLIGWGLVLGLHALHAFELLPWTTQDWEKRK
                     VRELVNEKLRH"
     gene            complement(3358..>3544)
                     /locus_tag="VGP50_13815"
     CDS             complement(3358..>3544)
                     /locus_tag="VGP50_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028506.1"
                     /translation="AEYRAGTRKRIYCFGQVFYDDDFGVGHFTNFGFSIVLDAANKPV
                     AIWTERHNETDRTAPVE"
BASE COUNT          594 a         1097 c         1208 g          645 t
ORIGIN      
        1 ggcgctgcgc gccgtgcccg ccgacacgct ctgctacgaa gagctgctcg ccgccgagcg
       61 cgatgaattt gcgtggccga tgttcgacga gcggctcgcc tcctcgctct gctacacctc
      121 cggcacgacc ggcgatccca aaggtgcgct ctatcagcat cgctcgaccg tgctgcactc
      181 ttacggctcg gcattgcccg attgcctcaa cctctcggcg cgcgacgtga tctgcccggt
      241 ggtgccgatg ttccatgtca acgcctgggg cttgccctat tcgtcgatgc tcgtcggtgc
      301 gaaactggtg ctcccggggc cggcgctcga aggcaagagt ctctatgagc tgttcgaggc
      361 cgagcaggtg acgatgagcg ccggcgtgcc gacggtgtgg ttcgcgctgc tgcaatatat
      421 gagcgagcgc ggcttgcgct tctccgcgct gcagcgcctc gtcatcggtg gctcggcctg
      481 tccgccggcg atgatcgagc gcttcgagga cgatcacggg atcgaggtgg tgcatgcctg
      541 gggcatgacc gagatgagtc cgatcggcac cttcgcgcag cccaaaggca agcatcgcgg
      601 cgccgacacg gcgcagctgc gcgtgatgaa gtcgaagcaa ggccgcgtca ttcccggcat
      661 cgatctgaag atcgtcgacg gcgccgggaa ggagctgccg tgggacggcg tcagcgccgg
      721 cgatctcatg gcgcgcggct ggtgggtgac gagcgcctat taccagaccg agccgggatc
      781 ggcgctgcag gacggctggt tcccgactgg cgacgtcgcg accatcgatt ccgacggcta
      841 catgcacatc accgatcgct cgaaagacgt gatcaagagc ggcggcgaat ggatcagctc
      901 gatcgagctc gagaacatcg ccgtcggcca tccggcgatc gccgaggcgg cggtggtcgg
      961 cgtgccgcat gccaaatggg gcgagcggcc gctgctggtg gcgcaggtga agtccggcgc
     1021 caaactcacg cgcgacgagt tgctggcgtt ctatcgcggc aagaccgcgg cgtggcggat
     1081 tccgagcgac gtcgtgttcg tcgatgcgct gccgcatacc gcgaccggca agctcctcaa
     1141 atcggagctg cgccggcgct acgccgatta ccggttgccg gcggagacct gaaacttctt
     1201 gtcgaggtcc tcgagcgcgg ctttgagctg gacgtccttc gccgtgccga tcgccgcagc
     1261 ctcgatcagc gtctcgctct tgaccggcgc cgggagaccg ggcgcggccg cggtcttgct
     1321 cgccgccttg gcgccggtgt cgagcgcgcc gggcagatcg gcctcgcgga ccagatcgac
     1381 atcggcggcc tgatcgccct tgggctgctc gacgatgcgg tcgggcgtga cgccctggcc
     1441 ttggatcgag cgaccgctcg gcgtgtagta gcgcgcggtg gtgaggcgca gcgcgccgtg
     1501 accctcgagc gggatgatcg tctgcaccga gcctttgccg aagctctgcg tgcccagcac
     1561 gtcggcgcga tggcgatcct gcagcgcgcc ggcgacgatc tcggcggcgg aggccgaggc
     1621 gccgttgatc agcaccacca tcggcacgcc gggaatccgg tcgccattgg cgggcgcggg
     1681 gaaggggcgg ttgtcgtcag gcgagcggcc gcggatcgag acggtgaggc cgccatcgag
     1741 aaaatccccc gagacatgca cggcctgatc gagcagcccg ccgggattgt tgcgcagatc
     1801 gagcaccagg cctttgagct tgccgtgcga tttggtcttg agctcgtcga gcgccttggt
     1861 gacttcgcgc tgcgtcgtct cggcgaagct cgagatgcgg atgtagccca tgccgtcggg
     1921 ctcgagcgcc gatttgaccg aatcgacatg gatgatcgcg cgcgtcagcg tcacgtcgaa
     1981 gggcgcggtg ttcttgcgcc cgatggtgat ctgcaccttg ctctcggcgg ggccgcgcag
     2041 cgcgtcgacc acgtccttga ggctgagccc ctcggtgatg cggccgttga tgcggaggat
     2101 ggcatcgccc ggccggatcc cggcgtgcga ggctggcgtg tcgtcgatcg gcgacagcac
     2161 caccggatga tcctggtcgc gcgtcagctc ggcgccgatg ccggcgaatt cgccggcgct
     2221 gtcgtcgagc atgtcctgat attcgcgctc ggtgaggaaa tcggaatgcg gatcgagccc
     2281 ggtcagcatg cccttcagcg aattgtcgac cagcgtgctg tcggtcaccg gatcgacgta
     2341 gctctgatgc accttgtcga gcactgagcg atagaaggcg agatcgctgg cgctcggtcc
     2401 cgccgtgtcg ggggcggagc cggagctggc ggcgttgcag ccggcgaggg cgagggcgag
     2461 caggacaaag aaccaaggcg agcgggtggg gcgaatcatt gacgctacga tgccttcatg
     2521 gtgatcgggt gttccggtcg tcccccggat tcggcgcgga acgggcaact atgccgcagg
     2581 aagcggggcg cgggcgagcg cttcaatgtc gcagcttctc gttcaccagc tcgcggacct
     2641 tgcgtttctc ccaatcctgc gtcgtccagg gcagaagctc gaaggcgtgc agcgcgtgga
     2701 ggccgaggac caggccccag ccgatcagcg gcgcgtagaa ccaggccggt ccgggcgaag
     2761 tcgaatcgat caggaagagg acgaagttga tgatcacata agcggcgaga tggctgtaga
     2821 aaattttcag cgagcgggcg cggccccagg cccatttctc cagctccgcg acgcgcgcgg
     2881 cttcgcgcac gcccgcgccc ggatggagct ccatgggctc gctctgttga ccgcgtatcg
     2941 ccatgcggca ggatcctgtc tccgagatgt gcaagtttag ccggtcccac gcatagccga
     3001 cagttaattg tctggacaat gtgggacaaa aactggtcgg agtgagagga ttcgaacctc
     3061 cggcccccgc ctcccgaaag ttatagcctg tatggcgcct ttcgacaaat ggctcgaacc
     3121 cgcgattttc ttccacacgg ctcaacgcat ttcgacgcta gtttccgcat ctgttcacgt
     3181 cgtagccgaa tgttagcgag aatgtaactg gcctacgcgt ttgttcgcgc ggctcgcatc
     3241 tcgcaggtga aaacgatcgc gcagagggcc gatcgaaaca tgccgcgatg ccgaagacag
     3301 ttagtcgccg ccccccgatt tctaggtgag ttgtgtgcgt cggtggcgct atcccaatta
     3361 ttcaactggt gcggtgcgat ctgtctcgtt gtggcgctct gtccaaatag ctacgggttt
     3421 gttggcggca tccaggacaa tcgaaaatcc gaaattcgtg aaatggccca cgccgaaatc
     3481 gtcgtcataa aagacctggc caaagcagta gattctttta cgagtcccgg cacgatactc
     3541 agca
//
LOCUS       DASYNS010000442         5255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3391652, whole genome shotgun sequence.
ACCESSION   DASYNS010000442 DASYNS010000000
VERSION     DASYNS010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3391652"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1784
                     /locus_tag="VGP50_13820"
     CDS             144..1784
                     /locus_tag="VGP50_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEV8028507.1"
                     /translation="MDEAAAERALRLRAGSEERLEQILTKLGLVHEKDVAEAMSSELG
                     VPMALPSDFPDEPVLESASPKFLRQAQVLPLRDLPDRLLLAVADPLNKYPVQSLALLA
                     GKPVEIRIATPSDVELAHERLYGGEKGAFGRIVDEIEQADDMGPDDDIDRLRDLASEE
                     PVIRLVNLLIARAVESRASDIHIEPFQDRLAVRYRIDGVLREGALPPARLRAAVVSRI
                     KIMAKLNIAERRLPQDGRIRIAIRGRDYDLRVATVPTLHGEGVTMRILDRSSLVEDFG
                     VLGFRPDTLKRYLDLLDQPQGILLVTGPTGSGKTTTLYTSLLRLNTPEKKIFTVEDPI
                     EYQLDGVNQVQVKPQIKLTFAEILRTLLRHNPDIIMVGEMRDFETAQVAIQAALTGHL
                     VLSTLHTNNAAGSINRLLDMKVDDYLVTSTVNGVMAQRLVRRLCEQCRKPRQALPELI
                     ERMGLRPLMNGRDTTLYDAVGCDACHSTGYRGQMAVIEVLALSDAIRRLVLNHAEVRE
                     IQRVAMEEGMRTMYQDGLLKALDGSTTVEEVLRVTRDV"
     gene            1799..3019
                     /locus_tag="VGP50_13825"
     CDS             1799..3019
                     /locus_tag="VGP50_13825"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV8028508.1"
                     /translation="MPQFRYVAVEGSSAPVEGRMEAVNKAAVIDRLHALGHTPIRVDQ
                     LAASPFASLDLAALFGPRRMPARQLALLTSQLATLLHAGLALDGALGILRQLATKPLD
                     RQCLKLLIERISGGATLSDAMAEQPNVFPGFYVSMIRAGEAGASLEAVLDRLSEFLER
                     SQAAREHIISALLYPIIVAVTCCLSIAVLFLFVVPRFRPVFEQAGNALPLAARGLLAV
                     SDFLQDFWWACILVPLLLAFLLYRQFKKPASRRRWDQRLLKLPGIGDLITKTEVVRFA
                     RTLATLLKNGVSLLAALTITRETITNGVFADAVGTIIERAKTGKGLAEPLAQTKVFPP
                     LVIHLVRVGEETGRADEMLFKVADIFEGETRRSIDRMLALLGPALTIGLGLIVAAVIG
                     SILTAVLSVYDLAM"
     gene            3031..3438
                     /gene="gspG"
                     /locus_tag="VGP50_13830"
     CDS             3031..3438
                     /gene="gspG"
                     /locus_tag="VGP50_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HEV8028509.1"
                     /translation="MTWRRRNERSEAGFTLVELLVVLAILGLLVAIATPQVIKYLGRA
                     KVSTAQIQIDGLKTALDMFLVDVGRYPTQQEGLEALVQNPGTMANWHGPYVKANSVPV
                     DPWGQPYQYRIPGQNGNDFDLYTLNGNNTVANQ"
     gene            3435..3893
                     /locus_tag="VGP50_13835"
     CDS             3435..3893
                     /locus_tag="VGP50_13835"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEV8028510.1"
                     /translation="MTEARVRGFTLIELVVVIAIAALMLALVLPSGQHRRQHDALSTG
                     AHEIASALRLTRSRAITANQPTSFIVDVATGFYRPAGASAAVPMPAGSHVTLYTAEEE
                     ALSEGVGTIRFYPDGSSSGGGIAISLGRERVQVLVDWLTGGVSIHDQPAG"
     gene            3874..4476
                     /locus_tag="VGP50_13840"
     CDS             3874..4476
                     /locus_tag="VGP50_13840"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV8028511.1"
                     /translation="MTSRPAEISPSPPLRGGEGRGEVGRSAPGRCHPSATPTLPHTRS
                     RTASAATPHFTPALSAPEGRRGGDFSCANREAGFTLIEILVAFTIVALMLGALYQLFA
                     TGLRSGATAENYESAVLLAESGIDALNGTALAVGDTSERLGRFERHTSVAPRADLLPT
                     EALSVPMLYELTVRVDWREGVRERSVTLSTLRAGPRDLPR"
     gene            4473..5108
                     /locus_tag="VGP50_13845"
     CDS             4473..5108
                     /locus_tag="VGP50_13845"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV8028512.1"
                     /translation="MIRSERGFTLLELLIAVSLLALLMGLLTAGLRAGTHSMARQSER
                     LDRSSRNVLVETFLRNELADARPFSTAGDSRSSTIAFTGREDGVTFVGPSPASVATGG
                     LQMLSVDFAKDGPARGEVVADWQPYGAGNGAGASPRRSLLLGHVQRAIFAYFGGEPGE
                     APSWHSDWENKTVLPSLVRLSVEFSDGELMPDLVIALRLADDANGQAGARQ"
BASE COUNT          835 a         1827 c         1720 g          873 t
ORIGIN      
        1 tgagcgccgc aaattctgag atggccggac cgcgcggctg gaggatcgcg ggttcgaccg
       61 gttgagagag gtaggccgct gtgcggcagg aacgcgtcgc gtttgaacga tcgctggcag
      121 cccggctggt agcgagcggc cgattggatg aagcggccgc cgagcgcgct ttgcgtttgc
      181 gcgcgggcag cgaggagcgg ctcgagcaga tcctgaccaa gctcggcctg gtccacgaga
      241 aggacgtcgc cgaagcgatg tcgagcgagc tcggcgtgcc gatggcgctg ccgagcgact
      301 ttcccgacga gcccgtgctc gaaagcgcca gcccgaaatt cctgcgccag gcgcaagtgc
      361 tgccgctcag agacctgccg gaccggctcc tcctcgcagt cgcggatccc ctcaacaaat
      421 atccggtaca gtcgctcgct ctcctcgccg gcaagcccgt cgaaatccgc atcgccacgc
      481 cgtccgatgt cgagcttgct catgagcgtc tctatggcgg cgagaagggc gcgttcggcc
      541 ggatcgtcga cgagatcgag caagccgacg atatggggcc cgacgacgac atcgaccggc
      601 tgcgcgacct cgcgagcgaa gagccggtca tccgcctcgt caatctcctc atcgcccgcg
      661 ccgtcgaaag ccgcgcctcc gacattcaca tcgagccttt ccaggaccgc ctcgccgtgc
      721 gttatcgcat cgacggcgtg ttgcgcgaag gggcgctgcc gccggcgcgt ctgcgcgccg
      781 ccgtcgtgtc gcgcatcaag atcatggcga agctcaacat cgccgagcgc cgcctgccgc
      841 aggatggccg catccgcatc gccatccgcg gtcgcgacta cgacctgcgc gttgcgacgg
      901 tgccgacgct tcatggcgaa ggcgtgacca tgcgcattct cgaccgctcg agcctggtcg
      961 aggatttcgg cgtgctcggc ttccgtcccg acacgctcaa gcgctatctc gacctgctcg
     1021 accagccgca aggcatcctg ctggtcaccg gacccaccgg cagcggcaag accacgacgc
     1081 tctacaccag cctgctccgc ctcaacacgc cggaaaagaa gatcttcacg gtcgaggatc
     1141 cgatcgaata ccagctcgac ggcgtcaacc aggtccaggt caagccgcag atcaagctga
     1201 ctttcgccga gatcctgcgc acgctgctgc gccacaaccc cgacatcatc atggtcggcg
     1261 agatgcgcga cttcgagacg gcccaagtcg cgatccaggc cgcgctcacc ggtcacctcg
     1321 tgctgtcgac gctgcacacc aacaatgcgg cgggctcgat caaccggctg ctcgacatga
     1381 aggtcgacga ctatcttgtg acctcgacgg tgaacggcgt catggcgcag cgtctcgtcc
     1441 gccgcttgtg cgagcagtgc cgcaagccgc gccaggcctt gcctgagctc atcgagcgca
     1501 tggggttgcg gccgctgatg aacgggcgcg acaccacgct ctatgatgcg gtcggctgcg
     1561 acgcctgcca cagcacgggg tatcgcggtc agatggccgt gatcgaggtc ctggcgctct
     1621 ccgatgccat ccgccgcctg gttctgaacc acgccgaagt gcgcgagatc cagcgcgtgg
     1681 ccatggaaga aggcatgcgg accatgtacc aggacggcct gctgaaggcg ctcgacggct
     1741 cgacgaccgt cgaggaagtg ctgcgcgtga cgcgcgatgt gtagagggca tccatagcat
     1801 gccgcaattt cgctacgtcg ccgtcgaagg cagctcggcg ccggtcgagg ggcgcatgga
     1861 ggccgtcaac aaggcggccg tcatcgaccg cctccacgcc ttgggccaca cgcccatccg
     1921 cgtcgaccag ctggcggcct cgcctttcgc cagcctcgat ctcgccgcgc tgttcgggcc
     1981 gcggcgcatg ccggcgcgcc agctcgcgct cctcaccagc cagctggcga cactgctcca
     2041 tgccgggctc gctctcgatg gagcgctcgg catcctgcgc cagctcgcca ccaaaccgct
     2101 cgaccggcaa tgcctgaagc tgctgatcga gcgcatcagc ggcggcgcca cgttgtcgga
     2161 cgcgatggcg gagcagccga atgtctttcc cggcttttat gtcagcatga tccgggccgg
     2221 cgaagccggt gccagtctcg aggccgtgct cgaccggctc tcggagtttc tcgagcgctc
     2281 gcaggcggcg cgggagcaca tcatctcggc actgctctat ccgatcatcg tcgccgtcac
     2341 ctgctgcctc tcgatcgccg tccttttcct cttcgtcgtg ccgcggttcc gtcctgtctt
     2401 cgagcaggcg ggaaacgcgt tgccgctcgc cgcccgcggc ctcctcgcgg tgtccgactt
     2461 cctccaggat ttctggtggg cttgcattct cgtgccgctg ctgctcgcct ttctcctcta
     2521 ccggcagttc aagaagccgg cgagccgccg ccgctgggac cagcggctgc tgaaactgcc
     2581 cggcatcggc gatctcatca ccaagaccga ggtcgtgcgc ttcgcccgca ccctggcgac
     2641 gctgctcaag aacggcgtgt cgctgctcgc cgcgctgacg atcacccgcg agacgatcac
     2701 caacggcgtc ttcgccgacg ccgtcggcac catcatcgag cgcgccaaga cgggcaaggg
     2761 cctcgccgag ccgctcgccc agaccaaggt gttcccgccg ctcgtcatcc atctcgtgcg
     2821 cgtcggcgag gagactgggc gggccgacga gatgctgttc aaggtcgccg acatcttcga
     2881 aggcgagacg cggcgcagca tcgaccgcat gttggcgctg ctcggcccgg cgctgaccat
     2941 cggccttgga ctgatcgttg ccgccgtcat cggctcgatc ctgacggcgg tcctgagcgt
     3001 ctatgatctc gcaatgtagg gagagcgacc gtgacgtgga gacggagaaa cgaacgttct
     3061 gaagcgggct tcacgctggt ggagctcctg gtcgtgctgg cgatcctcgg gctgctcgtc
     3121 gccatcgcca cgccgcaggt gatcaaatat ctcggacgcg ccaaggtgag caccgcgcag
     3181 atccagatcg atggtctaaa gaccgcgctc gatatgttcc tggtggatgt cggacgctat
     3241 ccgacgcagc aggaaggctt ggaagcgctg gtccagaacc ccggcacgat ggccaactgg
     3301 cacggtccct atgtcaaggc gaacagcgtg ccggtcgatc cctggggaca gccctatcag
     3361 tatcgcatcc ccggccagaa cgggaacgac ttcgatctct acacgctcaa cggcaacaac
     3421 accgtcgcga atcaatgacc gaggcgcgcg tccgaggctt tacgctgatc gagctggtgg
     3481 tggtgatcgc catcgccgcg ctcatgctgg ccttggtgct gccgagcggc cagcatcggc
     3541 gccagcatga cgcgctctcg acgggcgcgc atgagattgc atcggcgctg cgcctgacgc
     3601 gcagccgcgc catcaccgcg aatcagccga ccagcttcat cgtcgacgtc gcgaccggct
     3661 tctatcgccc ggccggcgcc agcgccgcgg tgccgatgcc ggccggcagt cacgtgacgc
     3721 tctacaccgc cgaggaggag gcgctgagcg agggcgtcgg caccatccgc ttctatcccg
     3781 acggcagctc gagcggcggc gggatcgcga tctccctcgg ccgcgagcgc gtccaggtgc
     3841 tggtcgactg gctgaccggc ggagtatcga tccatgacca gccggccggc tgagatttct
     3901 ccctctccgc ccctgagggg cggagagggt cggggtgagg tggggcgttc cgcgcccggg
     3961 cgctgccatc cgagcgcgac gccgaccctg ccgcacacaa gatctcgcac cgcgtccgcg
     4021 gcgacacccc acttcactcc agccctctcc gcccctgagg ggcggagagg gggcgacttt
     4081 tcatgcgcca atcgcgaggc cggcttcacc ctgatcgaga ttctggtggc gttcaccatc
     4141 gtggcgctga tgctgggcgc gctctaccag ctcttcgcga cagggctccg atcgggtgcc
     4201 accgccgaga attacgaaag cgcggtgctg ctggcggaat cggggatcga cgctctgaac
     4261 ggcacggcgc ttgcggtcgg cgacaccagc gagcgcctcg gccgcttcga gcggcacacc
     4321 agcgtggcgc cgcgcgccga cctcctcccc accgaggcgc tctcggtgcc gatgctctat
     4381 gagctcaccg tgcgcgtcga ctggcgcgaa ggcgtgcgcg agcggtccgt gacgctctcg
     4441 acgcttcgcg cgggaccgag ggacctgccg cgatgatccg ttcggagcgc ggcttcacct
     4501 tgctcgagct gctgatcgcg gtgagcctgc tggcgctgct gatgggcttg ctgacggcag
     4561 ggctgcgcgc cgggacccac agcatggcgc ggcaatccga gcggctcgac cgctcctcgc
     4621 gcaatgtgct ggtggagacg ttcctgcgca acgagctcgc cgacgctcgg cccttcagca
     4681 cggccggaga ttcgcgctcg agcacgatcg catttactgg ccgcgaggac ggcgtgactt
     4741 tcgtcggccc gtcgccggcg agtgtcgcca ccggcggctt gcagatgctc tccgtcgatt
     4801 tcgcgaagga cggcccggcg cgcggcgaag tcgtcgcgga ctggcagcct tacggcgcgg
     4861 gcaatggcgc gggcgcaagc ccccggcgca gcctgctgct cggccatgtc cagcgcgcca
     4921 tcttcgccta tttcggcggc gagcccggcg aggcgccaag ctggcattcg gactgggaga
     4981 acaagacggt gctgccgtcc ctggtgcggc tctcggtgga attctcggat ggcgagctca
     5041 tgcccgacct ggtgatcgcg ctgcgcctcg ccgacgacgc gaacggacag gcgggggctc
     5101 ggcaatgagt ctgcaaacgg cgttgtcgct tcatcagcag gggcggctcg cggaggccga
     5161 gtcgctctac caggcgctcc tcgatgccga ccgggaccat ttcgatgtgc tccacgcgct
     5221 cggcatcctg cgttgccagc agcgccgcta tgaag
//
LOCUS       DASYNS010000443         3766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4171145, whole genome shotgun sequence.
ACCESSION   DASYNS010000443 DASYNS010000000
VERSION     DASYNS010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4171145"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1808)
                     /locus_tag="VGP50_13850"
     CDS             complement(207..1808)
                     /locus_tag="VGP50_13850"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEV8028513.1"
                     /translation="MSASAYDYIVIGAGSAGCVLANRLSADSHCKVLLLEAGGSDRSL
                     YIRMPAGLARLTGPKFNWNYTTVPQAAMKGRRLYWPRGKTLGGSSSINAMIYIRGQAQ
                     DYERWRQLGNAGWSYSDVLPFFRLAEHNERLNDEWHGGAGPLNVADLRYHNPLSAVFV
                     EAAQECGIPLNPDFNGARQYGCGPYQVTQKDGARWSAASAYLHSVRTRPNLTVVLQAQ
                     AARLVIENGRAVAVRYTAARGGEMSEARAEREVLLAAGAINSPHLLLLSGIGPADELR
                     AAGAAVTHDLPGVGKNLQDHLNVNVLADATQPITLDGMASGLAALRVALQFELFKTGP
                     GTSNAAEAGAFVSSRGEQDAPDTQFHFIPAFVVDHGRQRIPGHGVTLHACCLRPESRG
                     EIRLHSSNPFDAPAIDPNYLASGYDLKVLIAGIRRGREILGAKAFRAYLGAERFPGAA
                     QHSDAQLEDFVRASAETEYHPVGTCKMGSDPLAVVDERLRVRGIAGLRVVDASIMPTV
                     VSGNTNAPVIMIAEKAAAMVLQDAA"
     gene            complement(1960..2754)
                     /locus_tag="VGP50_13855"
     CDS             complement(1960..2754)
                     /locus_tag="VGP50_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEV8028514.1"
                     /translation="MVQLTMRGRTRAALAQGGIRTIDAADLGEALSRGFDDFKAMPSH
                     FLFLSIIYPVIGLFLARIAIGNDVLPLLFPLAAGFALIGPFAAIGLYELSRRREQGHE
                     PFVWHGLAAFQSRQMFAIATVAVVLAVIFVAWLVTAQIVYQVTLGPAPPASLGAFIHD
                     LLTTPAGWALIIIGNGLGFFFAVVALSLSVVSIPLLLDRDVSALTAMETSLHTVFVNP
                     KPMAMWGSIVAGGLVVGSLPCFLGLVVVMPILGHSSWHLYRKVIES"
     gene            complement(2998..>3766)
                     /locus_tag="VGP50_13860"
     CDS             complement(2998..>3766)
                     /locus_tag="VGP50_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV8028515.1"
                     /translation="ERFGRAILELGGNNGMIVAPSADLELALRAILFAAAGTAGQRCT
                     TLRRLIVHEDVYDALVPRLKHAYAQIAIGNPLDPATLVGPLIDGTAFAAMQASLAEAL
                     RGGAAVTGGERVHGERWPGAYYVRPAIVEMAEQTPLMQRETFAPILYVLRYRSFEEAI
                     ALHNAVPQGLASAIFTNDLREAELFTAASGSDCGIANVNIGPSGAEIGGAFGGEKETG
                     GGRESGSDAWKAYMRRATNTINYSRTLPLAQGIRFDP"
BASE COUNT          626 a         1197 c         1352 g          591 t
ORIGIN      
        1 tggtacgcca gaggcggtga tcacgccgca gtcgctcgag cggctctatg gcgtgcaggt
       61 gcaggtcgtc gatctgcccg accaccagac gcatacttgc gtgccggcgg cgcgcggtct
      121 gcggcggcgg aattgaattg aaggaagtcg tcagttttca gttttcagtt cgaaactgat
      181 aactgacgac tgacgactga caactttcac gccgcgtcct gcagcaccat cgcggcggct
      241 ttctcggcga tcatgatgac cggggcgttg gtgttgccgc tgacgacggt cggcatgatc
      301 gaggcgtcga cgacgcgcag gccggcaatg ccgcgcacgc gcagccgctc gtcgacgacg
      361 gcgagggggt cgctgcccat cttgcaggtg ccgaccgggt ggtactcggt ctccgcgctg
      421 gcgcggacga aatcctcgag ctgcgcgtct gagtgctgcg ccgcgccggg aaagcgctcg
      481 gcgccgagat aggcgcggaa ggctttggcg ccgagaatct cgcggccgcg ccggatgccg
      541 gcgatcaaca ccttcaggtc atagccggag gcgagatagt tcggatcgat ggccggcgcg
      601 tcgaaggggt tgctgctgtg gagccggatc tcgccgcggc tctcgggcct gaggcagcag
      661 gcgtggaggg tcacgccgtg ccccggaatg cgctggcgcc catggtcgac gacgaaggcc
      721 ggaatgaagt ggaactgcgt atcgggcgcg tcctgctcgc cgcggcttga gacgaaggcg
      781 ccggcctcgg cggcgttgct ggtgccgggt ccggtcttga acagctcgaa ttgcagcgcg
      841 acgcgcagcg cggcgaggcc gctggccatg ccgtcgaggg tgatgggctg cgtcgcgtcg
      901 gccagcacgt tgacgttcag atggtcctgg aggttcttgc cgacaccggg caggtcgtgg
      961 gtcacggcgg cgcccgccgc gcgcagctcg tccgccgggc cgatgcccga gagcagcagg
     1021 agatgcggcg aattgatggc gccggccgcc agcagcacct cgcgctcggc gcgtgcctcg
     1081 gacatctcgc cgccccgcgc ggcggtataa cggacggcga cggcgcggcc gttctcgatg
     1141 acgaggcgcg cggcctgcgc ctgcagaacg acggtaaggt tgggccgcgt gcgcaccgag
     1201 tgaagatagg ccgaggcggc gctccagcgc gcgccgtcct tctgggtcac ctggtagggg
     1261 ccgcaaccat attggcgcgc gccgttgaag tcgggattga gcgggatgcc gcattcctgc
     1321 gccgcctcga cgaagaccgc gctcagcgga ttgtggtagc gcagatcggc gacattgagc
     1381 ggaccggcgc cgccgtgcca ttcgtcattg agccgctcgt tgtgctcggc aagccgaaag
     1441 aagggcagca cgtcgctata gctccagccg gcattgccga gctggcgcca gcgctcgtag
     1501 tcctgcgcct ggccgcggat atagatcatg gcgttgatcg agctcgagcc gccgagcgtt
     1561 ttgccgcgcg gccaatagag ccgccgcccc ttcatcgccg cttgcggcac ggtcgtgtag
     1621 ttccagttga acttcgggcc ggtgaggcgc gcgagcccgg ccggcatgcg gatatagagc
     1681 gagcgatcgc tgccgccggc ctcgagcaac agcaccttgc agtgcgaatc ggccgaaagc
     1741 cggttggcga ggacacagcc ggcagagccc gcgccgatga cgatgtagtc gtaagccgat
     1801 gcgctcatcc cgaccgtccc aggcatggat cggctgtttg actcgatcgg tgccgggagc
     1861 gcaagagcag gcagcgggcg ccgggctcaa gcctccgtga ccctctcccg catcgcggga
     1921 gagggtggcg ggagagggga ggttatcccg cgtcgaccct cagctctcga tgaccttgcg
     1981 atagagatgc cagctcgaat ggccgaggat gggcatgacc acgacgaggc cgaggaagca
     2041 cggcagcgag ccaaccacca ggccgccggc gacgatcgat ccccacatcg ccatgggctt
     2101 gggattgacg aataccgtat ggagcgaggt ctccatcgcc gtgagcgcgc tgacgtcgcg
     2161 atcgagcaac agcggaatgg acacgacgct gagcgacagc gccaccacgg cgaagaaaaa
     2221 gcccaggccg ttgccgatga tgatgagcgc ccagcccgcg ggcgtggtga ggaggtcgtg
     2281 gatgaaggcg ccgagcgagg ccggcggcgc cggccccagc gtcacttgat agacgatctg
     2341 cgccgtgacg agccaggcga cgaagatcac cgcgaggacg accgcgacgg tggcgatggc
     2401 gaacatctgg cgcgactgga aggcggcgag gccgtgccag acaaagggct catgcccctg
     2461 ctcgcggcgg cggctcagct cgtagagccc gatggcggcg aagggtccga tcagcgcgaa
     2521 gccggcggcg agcgggaaga gcagcggcag cacgtcattg ccgatggcga tccgcgcgag
     2581 gaagaggccg atcaccggat agatgatgct caggaacagg aaatggctgg gcatcgcctt
     2641 gaaatcgtcg aagccccgcg acaaggcctc tcccagatcc gccgcatcga tcgtgcgaat
     2701 cccgccttgc gccagcgccg cccgcgtccg gcccctcatc gtcagctgca ccatgtcctg
     2761 ccctccttga gagccgtcgg agcctctctg gccgtgtctc gtccaacctc gtcggttggc
     2821 gctccgcagg cgtgaataaa tgaatttaca tctttttttc catctataga agccccttcc
     2881 tcgatcattc tatagacaag tgcgtacctg ggcatggtga acgccgcctt gtggcatggg
     2941 cttctcggcc gccaggggct cggcggctct cgcgattgct tgcgcaatcg ctggctccta
     3001 ggggtcgaaa cggatgcctt gggcgagcgg cagcgtgcgc gagtaattga tggtattggt
     3061 ggcgcggcgc atataggcct tccaggcgtc gctgccgctc tcgcggccgc cgcccgtctc
     3121 cttctcgccg ccgaaggcgc cgccgatctc ggcgccggac ggaccgatat tgacgttggc
     3181 gataccgcag tcgctgccgg aagcggcggt gaagagctcg gcctcgcgca gatcgttggt
     3241 gaagatcgcc gaggcgaggc cctggggcac ggcattgtgg agcgcgatcg cctcctcgaa
     3301 gctgcggtag cgcagcacgt agaggatggg cgcgaaggtc tcacgctgca tgagcggcgt
     3361 ctgctccgcc atctcgacga tggccgggcg cacgtaatag gcgccgggcc agcgctcgcc
     3421 atgcacgcgc tcgccgccgg tgaccgccgc gccgccgcga agcgcctcgg cgaggctcgc
     3481 ctgcatggcc gcaaaggcgg tgccgtcgat cagcggcccg accagcgtgg cgggatcgag
     3541 gggattgccg atggcgatct gggcataggc atgcttgagc cgcggcacca gggcgtcgta
     3601 gacgtcctca tgcacgatca gccgccgcag cgtcgtgcag cgctggccgg ccgtgccggc
     3661 ggcggcgaag agaatggcac ggagcgcgag ctcgagatcg gccgacggtg ccacgatcat
     3721 gccgttgttg ccgccgagct cgaggatcgc ccggccgaag cgctcg
//
LOCUS       DASYNS010000444         2748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2923965, whole genome shotgun sequence.
ACCESSION   DASYNS010000444 DASYNS010000000
VERSION     DASYNS010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2923965"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VGP50_13865"
     CDS             complement(<1..620)
                     /locus_tag="VGP50_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV8028516.1"
                     /translation="MAKNNVGTISQVIGAVVDVKFDGDLPNILNALNAQNQGKTLVLE
                     VAQHLGENTVRTIAMDATEGLVRGAEVVDTGGPIMMPVGPETLGRILNVVGEPVDERG
                     PVITKMRSPIHRPAPEFIDQSTEAEILITGIKVVDLLAPYARGGKIGLFGGAGVGKTV
                     IIMELINNIAKKHGGYSVFAGVGERTREGNDLYHEMIESKVIQLDGP"
     gene            complement(775..1662)
                     /locus_tag="VGP50_13870"
     CDS             complement(775..1662)
                     /locus_tag="VGP50_13870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV8028517.1"
                     /translation="MATLKELRLRIRSVRSTQKITSAMKMVAASRLRRAQEQAEASRP
                     YASRMERMLASLAANLRGQANAPPLLAGTGRDAVHLLVVATSDRGLAGGFNASILRET
                     RRTIRALQGAGKTVKLLTIGRKGRDSLRRDVSRLIAESRTDVGRPSLTFEQARAIADR
                     VLALFVAGEFDVCTIIYNRFRSAMTQIVTRQQLIPFAPPETAAAGAATASGAVYEFEP
                     EEAEILAELLPRNLAVQVFTALLENAASEQGARMTAMDNATRNAGDMIDRLQIVMNRT
                     RQAAITRELIEIISGAEAL"
     gene            complement(1674..>2748)
                     /gene="atpA"
                     /locus_tag="VGP50_13875"
     CDS             complement(1674..>2748)
                     /gene="atpA"
                     /locus_tag="VGP50_13875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV8028518.1"
                     /translation="IDALVPIGRGQRELIIGDRQTGKTAVAIDAFLNQKAVNASGDEK
                     RKLYCIYVAIGQKRSTVAQIVKTLQDFGALDYSIVVAATASEPAPLQYLAPYTGCAMG
                     EFFRDNGMHALIVYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKM
                     NDKFGGGSLTALPVIETQAGDLAAYIPTNVISITDGQIFLETELFYRGIRPAINVGAS
                     VSRVGSAAQIKAMKQVAGRIKLELAQYREMAAFSQFASDLDQSTQRLLARGARLTELL
                     KQPQFSPMPVEEQVVSIFAGTRGYLDKIDIAQVTRFEAAMLGELRSRKPQLLQAIREK
                     RELVPEIEKELAAFLDGFVKGFA"
BASE COUNT          429 a          899 c          933 g          487 t
ORIGIN      
        1 gggccgtcga gctggatgac tttcgactcg atcatctcgt gatagaggtc gttgccttcg
       61 cgcgtgcgct cgccgacgcc ggcgaagacc gagtagccgc catgcttctt ggcgatgttg
      121 ttgatgagct ccatgatgat gacggtcttg ccgacgccgg cgccgccgaa cagcccgatc
      181 ttgccgccgc gcgcataagg cgccagcaaa tccacgacct tgatgccggt gatgaggatc
      241 tcggcctcgg tcgactggtc gatgaattcg ggtgccggtc tatgaatggg cgaacgcatc
      301 ttggtgatga cggggccgcg ctcgtcgacg ggctcgccca cgacattgag gattcgcccc
      361 agcgtctcgg gtccgaccgg catcatgatg gggccgcccg tgtccaccac ttcggcgccg
      421 cgcacgaggc cttcggtcgc gtccatggca atggtgcgta ccgtgttctc gccgagatgc
      481 tgcgccactt cgagcaccag cgtcttgccc tggttctgcg cgttcagcgc gttcaggatg
      541 ttcggcagat cgccgtcgaa cttcacgtcg acgacggcgc cgatgacctg cgagatggtg
      601 ccgacgttat tctttgccat cgctgctttt cctctggttc ccgggcaggt caccccccac
      661 ccctccctcc ccctcaaggg gggagagaga agatgaagct acccgacagt cccctccccc
      721 cttgaggggg agggctaggg aggggggtga agcccgtgtg atgagtcgca tctatcagag
      781 cgcctcggca ccggagatga tctcgatgag ctccctggtg atggcggcct ggcgcgtccg
      841 gttcatgacg atctggagcc gatcgatcat gtcgccggcg ttgcgcgtgg cattgtccat
      901 cgccgtcatg cgcgccccct gctcgctcgc cgcattctcg aggagcgccg tgaacacctg
      961 gaccgcgagg ttgcgcggca gcagctcggc gaggatctca gcctcttcgg gctcgaactc
     1021 gtagacggcg ccgctggccg tggcggctcc cgccgcggcg gtctcgggcg gcgcgaaggg
     1081 gatgagctgc tgccgggtga cgatctgcgt cattgccgag cggaagcggt tgtagatgat
     1141 cgtgcagacg tcgaactcgc cggcgacgaa aagtgccagc acgcgatcgg cgatggcgcg
     1201 cgcctgctcg aaggtgaggc tgggacggcc gacatccgtg cggctctcgg caatgagccg
     1261 gctcacatcg cgccgcaggc tgtcgcgccc cttgcggccg atggtcaaga gcttcaccgt
     1321 cttgcccgca ccctggagcg cgcggatcgt ccgccgcgtc tcgcgcagga tggacgcgtt
     1381 gaagccgccg gcgagaccgc gatcggaggt cgccaccacg aggagatgca ccgcatcgcg
     1441 ccccgtgccg gccagcaatg ggggcgcatt ggcctggccg cgcagattgg ccgcgagcga
     1501 ggccagcatg cgctccatgc gcgatgcgta gggccgcgac gcctcggcct gctcctgggc
     1561 gcggcgcagc cgcgacgccg ccaccatctt catcgccgag gtgatcttct gcgtcgaccg
     1621 gacgctgcgg atccgcagcc gcaattcctt gagggtggcc atgacctcct acgctacgcg
     1681 aagcccttga cgaacccgtc gaggaaggca gcgagctcct tttcgatctc gggaacgagc
     1741 tcgcgcttct cgcggatcgc ctggagcagc tgtggcttgc gcgagcggag ctcgcccagc
     1801 atcgccgcct cgaagcgcgt cacctgcgcg atatcgatct tgtcgaggta gccgcgcgtg
     1861 ccggcgaaga tcgacacgac ctgctcctcg accggcatgg gcgagaattg cggttgcttc
     1921 aggagctcgg tgagccgggc cccccgcgcc agaaggcgct gcgtcgactg atcgaggtcg
     1981 gaggcgaatt gcgagaaggc ggccatctcg cggtattgcg cgagctcgag cttgatgcgg
     2041 cccgcgacct gcttcatcgc cttgatctgc gccgccgagc cgacgcggct caccgacgcg
     2101 ccgacattga tggccgggcg gatgccccga tagaagagct cggtctcgag gaagatctgg
     2161 ccgtcggtga tcgagatcac attggtcggg atgtaggccg cgaggtcgcc ggcctgggtc
     2221 tcgatcaccg gcagcgccgt cagcgagccc ccgccgaact tatcgttcat cttggccgcg
     2281 cgctcgagga gccgcgaatg caggtagaaa acgtcgcccg gataggcttc gcgccccggc
     2341 gggcggcgca gcagcagcga catctgtcgg taggcgacgg cttgcttcga gagatcgtca
     2401 taaacgatca acgcatgcat cccgttgtcg cggaagaact cgcccatcgc acagcccgta
     2461 tagggcgcca gatattgcag aggcgccggc tccgacgcgg tcgcggcgac gacgatcgag
     2521 tagtcgagcg cgccgaagtc ctggagcgtc ttgacgatct gcgccacggt cgagcgtttc
     2581 tgcccgatcg ccacatagat gcagtagagc ttgcgcttct cgtcgcccga cgcgttcacc
     2641 gccttctggt tgaggaaggc gtcgatggcg acggcggtct tgccggtctg gcggtcgccg
     2701 atgatgagct cgcgctggcc gcggccgatc ggcaccagcg cgtcgatc
//
LOCUS       DASYNS010000445        12560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4637918, whole genome shotgun sequence.
ACCESSION   DASYNS010000445 DASYNS010000000
VERSION     DASYNS010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 12560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12560
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4637918"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(17..203)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            357..1091
                     /locus_tag="VGP50_13880"
     CDS             357..1091
                     /locus_tag="VGP50_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028519.1"
                     /translation="MLAIAVAALAGCAPDYSPNTYAANAVQQANKVEPGVVVGYRQVQ
                     ISASGTVGAVTGGAAGGILGSQADVSGVSTALGALGGTLVGGIVGTTIEHTTGDTTGW
                     EYIVRKPTGELLAVTQREPSPIPIGQKVLVITGNQARVIADYSVAVEPPPASSSDKEK
                     ADAKPPTASASTEPAAPSPPAASAPSASPPPAPSSTSVAASPGEPPAPSGKSAEAAPA
                     TVADPTPTAAAHAAPGDDPAPETSSP"
     gene            1824..2696
                     /locus_tag="VGP50_13885"
     CDS             1824..2696
                     /locus_tag="VGP50_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV8028520.1"
                     /translation="MAWAGTGIVWTVSGGVPRMLTMGEAIAAPADGFTFVQISDTHIG
                     FNKEANPDAAGTMQAALDRIATLPKRPAMILHTGDVTHLSKPAEFDAAAQIMKGAKFD
                     AHYVPGEHDVIGDDGKQFFERFATQANPGGWYSFDQGGVHFIGLDNVQNFEKSHAGGF
                     GSDQLEWLEKDLKGRGASTPIVVFTHIPMWPIYPQWGWSTEDAPQALANLKRFGSVTV
                     LNGHIHQVVQQIEGNVTYRTAMSTAFPQPAAGEGPGPGPLKVPADKLRGVLGIREVTI
                     VAEKPAAVSDATLA"
     gene            2713..3069
                     /locus_tag="VGP50_13890"
     CDS             2713..3069
                     /locus_tag="VGP50_13890"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEV8028521.1"
                     /translation="MITSFQRQFLAAAVAAVLLAGAGGAVAQQVATAGPAQISIDNFD
                     FKPSTLTVAAGTTVTWVNNDEEPHTIVNVGQPTRVFKSGGLDGGDTYSFVFDKPGTYD
                     YICSIHPHMSGQIIVK"
     gene            complement(3134..3901)
                     /locus_tag="VGP50_13895"
     CDS             complement(3134..3901)
                     /locus_tag="VGP50_13895"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV8028522.1"
                     /translation="MPALVWLLAGRSTGTAAADDDCNGGGIDIVTDRPDVTNSAVVMC
                     RGSLQIENGMNWTARHDTAVIDGPNSRVRLGVAPDTEVLVDLPDYFLRARGPGAAGFG
                     DLSPAVKRQLGPLPGDIELSATAGLQLPTGAARIAGAGYGGYLQFPWSKEIGGGWGVS
                     GMVTAFWIPGASQSNPTWEPTVVLERAVGSRADLFVEYVSDHPRRGVSSQIFNSGGAY
                     RPTATQQIDFHVGIGLNHVAPDYFFGVGYSIRFDSLF"
     gene            complement(4095..7241)
                     /locus_tag="VGP50_13900"
     CDS             complement(4095..7241)
                     /locus_tag="VGP50_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEV8028523.1"
                     /translation="MISAIFIDRPRLAFVISIVITLAGLLAIAAIPVAQFPDIVPPQV
                     TVTATYPGADAEVVETTVAQPIEQQINGVDNALYYQSTSGADGSYTLNVTFALGTDPD
                     IDTVNVQNRTSLAVPQLPDEVSRQGLSVRKKSAALLQVVLLYSPKGTFDSLYLSNYGT
                     INVIDALSRIKGVGQASQFGPLDYSLRVWLDPDKLTALNLTPNDVITAVQNQNVQAAL
                     GRIGAAPVTSDQQFQLTIKTKGRLTRPEEFAEIVLRANPNGSVVRVKDVARVDMGAKS
                     SDRYSRFNGAPTAAIGIYQLPGANAVEVARDVRNVMDALAQRFPQDLSYRVFYDTTVF
                     VNATITEVIRTLGEAFVLVGVVVFLFLGKIRTTIIPLVAVPVSIIGTFAVMLLIGYSA
                     NTVSLLALVLAIGIVVDDAIVVIENVERVIDEDPHLSIADATKKAMGEITAPIIAITL
                     VLLSVFVPVAFIPGISGQLFRQFAVAVSVAMLISAVNALSLSPALCSVLLKRGQPSRG
                     PLRYVLRGIDSVRDGYVSVVRRLVRVAAVGVVAVIAALAGSAGLFTKAPQSFLPDEDQ
                     GAFFAAMRLPEGTSVNRTEALVAQVEDMIRPIAGVEGVLSVVGFNFIDGLASSNQAFF
                     VVRLKPYEERKAASESVGAIIQTLRPRLAALPGAIAFPFNLPPIIGLGSTGGFQYVLE
                     ALQGQPPADIAQTLRAALVAANQQPELAGVFSTFAADTPQLYLEIDRDKAQALGVRIS
                     DIFNALQATLGGFYVNDFNVFGRTWQVNVQAETPFRKSVDDIYRIYVRSGEGAMIPMR
                     ALATARAVQGPQALIRYNGFRAAIINGAAKPGSSSGEALAAMERLSATTLPAGYGYEW
                     TGTALQEKFASGKTPIVLGLAVLFAYLFLVALYESWNIPVPVLLSVSVGILGAIAAVV
                     LAARSFDVYAQIGLVVLVALAAKNGILIVEFAVDQRRSGRSIADAAIEGARLRFRPVM
                     MTSFAFILGLLPLALAAGAGAQSRRAVGTPVVGGMLAAACLGVFVIPMLYVVFQSLRE
                     RSGRRAAPQAAPQAATGD"
     gene            complement(7250..8434)
                     /locus_tag="VGP50_13905"
     CDS             complement(7250..8434)
                     /locus_tag="VGP50_13905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV8028524.1"
                     /translation="MHGGRKNRWSQRGAGAHRGRARLLRAAVVLLLGGLAACNRSGVA
                     QAPAPPPAVGVQPAMMKGVSRSAEFVGRIKAIDIVQLRARVEGFLDKVAFTEGDEVKP
                     GQLLYQIETVQYEAQLEQAKANLAAARSQALNAELQYNRVAELVKRQNISQATVDQDK
                     ANLDGARASVLQNEAAVTLAQTNLGYTTIKAPVQGRIGRTGFTQGNLVNPASGVLATI
                     VSQDPIYVLFPVSVRQAEEARAAASGQDARAFAAQLEIVLRLPDGREYQHPGAWNYTD
                     PQVDQQTDTVTLRGTVPNPERLLVDGQFVTVLVRERAEQPRLVIPQAALLADQAGSYV
                     LVVDGDEKAEIRRVATGPEEHADIVVESGLKEGERVIVEGVQKVRPGQVVQATPVVTK
                     GG"
     gene            complement(8535..9044)
                     /locus_tag="VGP50_13910"
     CDS             complement(8535..9044)
                     /locus_tag="VGP50_13910"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEV8028525.1"
                     /translation="MGVSGVGKSTVANALAARLGWPFEEGDALHPEANVAKMHAGIPL
                     TDADRQPWLEAVAAWIDHQRALKQPGIITCSALKRAYRALIIGDRPEARLIYLRGSRA
                     LIAERLAGRHGHFMPASLLQNQLDTLEEPSPDEDALTVDAGPPPGEIAQEIIRLLGAS
                     AVVGKRAKG"
     gene            complement(9090..9788)
                     /gene="rpiA"
                     /locus_tag="VGP50_13915"
                     /pseudo
     CDS             complement(9090..9788)
                     /gene="rpiA"
                     /locus_tag="VGP50_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
     gene            complement(9785..10465)
                     /gene="rpe"
                     /locus_tag="VGP50_13920"
     CDS             complement(9785..10465)
                     /gene="rpe"
                     /locus_tag="VGP50_13920"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV8028526.1"
                     /translation="MKVLIAPSVLSADFARLGDEVRAVDAAGADWIHVDVMDGRFVPN
                     ISIGPTVVEAIRRSTAKPLNVHLMIVEPERYLTRFAKAGADHLLVQAEPGSTTHLHRV
                     LTEIRALGKKAGVVVDPASPPELIEYVLHLADIILVMTVNPGFGGQAFLPEMLPKIRR
                     IRAMCRERGLDPVIEVDGGEDVATARQAAAAGANAIVAGSAIFGAPDYKAAIAAIRAS
                     ASAGAQQS"
     gene            complement(10468..11211)
                     /gene="pgl"
                     /locus_tag="VGP50_13925"
     CDS             complement(10468..11211)
                     /gene="pgl"
                     /locus_tag="VGP50_13925"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEV8028527.1"
                     /translation="MTSKDRLAPTEKVEVFADPAALARHVAEWMTRAALAAERPFRVS
                     LSGGSTPETLYALLASPAFKSRFPWPRVSWYWGDERFVPQDHPDSNYRMARAAMLSKA
                     PIPPENIHPVPVDGTPDDAARRYERILQDAYGGRVLDPARPLFDITLLGLGPDGHTAS
                     LLPGEPVLEERGRWVAAVAHGRPEIRITMTYPAIESSRAVAFLVAGREKAPILRAIRA
                     GGSQVPAARVRPVGALFWFVDRAAAGDAA"
     gene            complement(11539..12357)
                     /locus_tag="VGP50_13930"
     CDS             complement(11539..12357)
                     /locus_tag="VGP50_13930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010518192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV8028528.1"
                     /translation="MAEKRKISLVLADVDGTLVTEDKVLTPRAQAAVKALGEADIRFA
                     ITSGRPPRGMAMLFDALELTTPVAGFNGGIFVKRDLSILAENTLPADVARQAIDLIRH
                     HGLDAWVYHGNDWLITKPGAPHVAREAWTVKFEPKIVADFDDMLDGAAKIVGISDDLD
                     CVRRCEADAQAAFGERATASRSQPYYLDVTSKDANKGAVVAYLSQHLEVPPEEIATIG
                     DQPNDVLMFKRSGFSIAMGNASDQVKAQASATTDSYNDEGFAKAMERFVLGRLG"
     gene            complement(12373..>12560)
                     /locus_tag="VGP50_13935"
     CDS             complement(12373..>12560)
                     /locus_tag="VGP50_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV8028529.1"
                     /translation="DATLFQRADNIESGWQAVQPILDAWAENAPKDFPNYAAGSSGPE
                     AADALLAQDGHAWRPLG"
BASE COUNT         2007 a         4319 c         4215 g         2019 t
ORIGIN      
        1 aaatgcttcc atcgaggtgc tcccggccga gcggcagatc aacggctgat ctcctcccgc
       61 tcggcggagg gtcagtccgt tggtctcgcc atgcccgaag gccccgcgcg ccatggcaat
      121 ccttgccaag catgttgacg cgcggcccgg tctcgtcgcc gggcggctac tccccaatgg
      181 gtcggaatgt tctagtcaaa ttgccgcagg cgcgcaatcg acggcatcgc gggaaatgtg
      241 acgcgccgcc cagattcgac catgaaattg ccccgtgaca cccgtgcggc atccctttaa
      301 agaagcggac gggatctggg cgggggtttt tggtgttgcg ctttcccacg ttaggcttgc
      361 ttgcgatcgc tgtcgccgcg ctcgctggtt gcgcgcccga ctattcaccc aacacttacg
      421 cggccaatgc cgtccagcag gcgaacaagg tcgagccggg tgtcgtggtc ggctatcgtc
      481 aggtgcagat cagcgcgagc ggcaccgtcg gggcggtgac cggcggcgct gccggcggca
      541 ttctcggatc gcaggccgat gtcagcggcg tcagcacggc gctcggtgcc ctcggcggga
      601 ctctcgtcgg cggcatcgtc ggcacgacga tcgagcacac gacgggcgat acgaccggct
      661 gggaatacat cgtccgcaag cccactggcg agctgctcgc ggtcacgcag cgcgagccgt
      721 cgccgatccc gatcgggcag aaggttctgg tgatcaccgg caaccaggcg cgcgtgattg
      781 cggattactc ggtcgccgtc gaaccgccgc cggcatcgtc ctcggacaag gagaaggccg
      841 acgcgaagcc gccaacggct tcggcctcga ccgagcctgc ggcgccaagc ccaccagctg
      901 cgtccgctcc ctctgcatcc cctccgcccg cgccctcttc gacctccgtt gcggcgtctc
      961 ccggtgaacc accggcgccg tcgggcaagt cggcggaggc cgcacccgca acggttgctg
     1021 accctacccc caccgcggcg gcacacgctg cgccgggcga tgacccggca ccggaaacat
     1081 catcgccgta agagcgtggg cagagtcttc tctgcgccat tcgctgctcg gcacgctgcg
     1141 tcatccgcga ttcttccgcc caccggctcc agcagacggt cggacgcgtc atcgcccggg
     1201 aacgatatct cgatacgccg ctcctgcacg gtaatgtctt gcgacgtacc aaaagtcgtc
     1261 agcatgttca cgagcttgag ccgctcgtct ccgaccttga gctccagagc gatcgtcggc
     1321 tcatacgtcc atccaatccc tctttgaatg cgtcacagat cgcgtcacgc tgcgcttgaa
     1381 ttttatcgcc ggcgcgcgcc gtagcgcgtt catactcccg caacccgggc gttttccggg
     1441 aagaaaaatc gccgccggct gatcttcaca gacgacggcc cgctctctcc tcgtcggccg
     1501 tcgacccgct caagcgcccg cggcgggtct tgcgacaccg cgccgagtct gcgcttttac
     1561 gccgctttcc tccaggggcg gcgttcgagc cgggctcggc gcgggagtcg tgcatcgccg
     1621 atgcccgtct ttgccgcccc gcgttatccc cgcattatcc atgtcttgtc gtccgcgcgc
     1681 tcttttccgc gtgttacatg tcggggaata aatccatcac cgccctggtc ttgcatctca
     1741 gtcaatcgcg acacaggagc ggtatcatgg acacggaaaa gccggatagc cccggcacga
     1801 atcgccgaac attactcaaa tgcatggcat gggcgggaac cggcatcgtc tggacggtct
     1861 cgggcggcgt cccgcgcatg ctgacgatgg gcgaggcgat cgccgcgccc gccgacggct
     1921 tcaccttcgt ccagatcagc gacacccata tcggcttcaa caaagaagcc aatcccgatg
     1981 ccgccgggac gatgcaggcg gcgctcgacc gcatcgcgac attgccgaag cgccccgcga
     2041 tgatcctcca caccggcgac gtcacgcatc tgtcgaagcc ggccgagttc gacgccgccg
     2101 cgcagatcat gaaaggcgcg aaattcgatg cgcattacgt gccgggcgag cacgacgtga
     2161 tcggcgacga cggcaagcag ttcttcgagc gcttcgcgac gcaggccaat ccgggcggct
     2221 ggtacagctt cgatcagggc ggcgtgcatt tcatcggcct cgacaacgtg cagaatttcg
     2281 agaagtcgca cgccggcggc ttcggcagcg accagctcga atggctcgag aaggacctga
     2341 agggccgcgg cgccagcacg ccgatcgtcg tcttcaccca tatccccatg tggccgatct
     2401 atccgcagtg gggatggtcg accgaggacg cgcctcaggc gctcgccaat cttaagcgct
     2461 tcggctcggt gaccgtgctc aacggccaca tccatcaggt cgtgcagcag atcgaaggca
     2521 acgtgaccta ccgcaccgcc atgtcgacgg cgttcccgca gcccgccgcc ggcgaaggcc
     2581 cgggtccggg cccgctcaag gtccctgccg acaagttgcg cggcgtgctc ggcatccgcg
     2641 aggtgacgat cgtcgcggag aagccggccg cggtgagcga cgcgacgctg gcgtgagcag
     2701 ggagttgcac ccatgatcac ctcttttcag cgccaattcc tggccgcggc ggtggcggcg
     2761 gtgttgctcg ccggcgccgg cggcgccgtt gcgcaacaag tggccacggc cggacccgct
     2821 caaatctcga tcgacaattt cgacttcaag ccgagcacgc tgacggtcgc ggccgggacg
     2881 acggtcacct gggtcaacaa cgacgaggag ccgcacacca tcgtcaatgt cggccagccg
     2941 acccgcgtct tcaaatcggg tgggctcgac ggcggcgata cctactcctt cgtcttcgac
     3001 aagcccggga cctacgacta catctgctcg atccatccgc acatgtcggg ccagattatc
     3061 gtcaagtagc cgtgtcgaac ggaacgcgcg ccctcatcgg ccgtcgcatc agcggccggt
     3121 gagggcgctc ttgtcagaac aggctgtcga agcgaatgga gtagccgacg ccgaagaagt
     3181 aatcgggcgc cacgtgattg agcccgatcc ccacatgaaa atcgatctgc tgcgtcgccg
     3241 tcggtcgata ggcgccgccc gaattgaaga tctggctgga gacgccgcgg cgcggatggt
     3301 ccgagacata ttcgacgaag agatccgcgc gcgaaccgac cgcgcgctcc agcacgactg
     3361 tgggttccca ggtgggattg ctttgcgacg cacccggaat ccagaaagcc gtgaccatgc
     3421 cgctcacgcc ccagccgccg ccgatctcct tcgaccaggg gaattggaga tagccgccat
     3481 agccggcgcc cgcgatcctc gccgctcccg tcggcaattg caggccggcg gtcgccgata
     3541 attcgatgtc cccgggcaac ggcccgagct ggcgcttgac cgccggcgac aagtccccga
     3601 accccgccgc tcccggcccg cgcgccctca ggaaatagtc cggcagatcg acgagcacct
     3661 cggtgtcggg ggcgacgccg agccgcactc tgctgttggg cccgtcgatc acggcggtgt
     3721 cgtgccgcgc cgtccagttc atgccgtttt cgatttgcag gctgccgcga cacatcacca
     3781 cggccgaatt cgtcacgtcg ggccggtcgg tgacgatgtc gatcccgccg ccattgcagt
     3841 cgtcatcggc agctgccgtc cccgtgctgc gtcctgccaa gagccagacg agcgccggca
     3901 cgaacgcgat cggtactcgg ttgcgcaaag tgcccgcttt gtttcgacgg tccgggtgag
     3961 cccggcgggt tcatctctcg gatgctctga ctcggaaagc gcgtccgata ttcctgcgag
     4021 gtgttgtcgc ggcgaacttc ttcttggcgg cagggcgccg gccggggccg cgcgactccc
     4081 gttcgatccg aaattcaatc gcccgttgcc gcctgcggcg cggcttgcgg cgctgcgcgc
     4141 cggccgctcc gctcgcgcag cgactggaac acgacataga gcatcgggat cacgaagacg
     4201 ccgaggcacg ccgccgcgag catgccgccc accaccggcg tacccacggc acgccggctc
     4261 tgggcgccgg cacccgcggc cagcgccagc ggcagcagcc cgaggatgaa agcgaagctc
     4321 gtcatcatca ccgggcggaa gcgcagccgc gccccttcga tcgccgcgtc ggcgatgctg
     4381 cgcccgctgc gccgctggtc gaccgcgaat tcgacgatca ggatgccgtt cttcgcggcg
     4441 agcgcgacca gcacgaccag cccgatctgc gcatagacgt caaagctgcg ggcggccagc
     4501 accacggcgg cgatcgcgcc gaggatgccg acgctgaccg agagcaggac ggggacggga
     4561 atgttccagc tctcatagag cgccaccagg aagagatagg cgaagagcac ggcgaggccg
     4621 agcacgatcg gcgtcttgcc gctcgcgaac ttctcctgga gcgccgtgcc ggtccattcg
     4681 tagccatagc cggcgggcag cgtcgtcgcc gacaggcgct ccatggcggc cagcgcctcg
     4741 ccggagctgg agccgggctt ggccgcgccg ttgatgatgg ccgcgcggaa gccgttgtag
     4801 cggatcagcg cctgcggtcc ctggaccgct cgcgccgtgg cgagcgcgcg catcgggatc
     4861 atcgcgccct cgccgcttcg cacatagatc cggtagatgt catcgaccga tttgcggaac
     4921 ggcgtctcgg cctggacatt gacctgccag gtccggccga acacgttgaa gtcgttgaca
     4981 tagaatccgc ccagcgtcgc ttgcagcgca ttgaagatgt cgctgatgcg cacgcccagc
     5041 gcctgcgcct tgtcgcggtc gatctcgaga tagagctgcg gcgtgtccgc ggcgaaggtg
     5101 ctgaacacgc cggcgagctc gggctgctga ttcgccgcca ccagcgccgc gcggagcgtc
     5161 tgcgcgatgt cggcgggcgg ctgtccctgc agcgcctcga gcacatattg gaagccgccg
     5221 gtgctgccga ggccgatgat cggcggcagg ttgaagggga aggcgatggc gcccggcagc
     5281 gccgcgaggc gcggccggag cgtctggatg atggcgccga cgctctccga cgccgctttc
     5341 cgctcctcgt aaggcttgag acgcacgacg aagaacgcct ggttggacga ggcgaggccg
     5401 tcgatgaaat tgaagccgac gacggagagg acgccttcga cgccggcgat cggccggatc
     5461 atgtcctcga cctgcgccac cagcgcttcc gtgcggttga ccgaggtgcc ttccggcagc
     5521 cgcatcgcgg caaagaacgc gccctggtcc tcgtccggca ggaagctctg cggtgccttg
     5581 gtgaagaggc cggcggagcc ggcgagcgcg gcgatgaccg cgaccacgcc gacggccgcg
     5641 acgcgcacca ggcggcgcac cacggagaca tagccgtcgc ggacgctgtc gatgccgcgc
     5701 agcacgtagc ggagcgggcc gcggctcggc tgtccccgct tcagcagcac cgagcacagc
     5761 gccgggctca ggctcagcgc gttcacggcg gagatcagca tcgcgaccga caccgccacg
     5821 gcgaactggc ggaagagctg gccgctgatg ccggggatga acgcgaccgg cacgaacacc
     5881 gacagcagca cgagggtgat ggcgatgatc ggcgcggtga tctcgcccat ggctttcttg
     5941 gtggcatcgg cgatcgagag atgcgggtcc tcgtcgatga cgcgctcgac attctcgatg
     6001 accacgatcg cgtcgtcgac gacgatgccg atggcgagca ccagcgccag gagcgacacg
     6061 gtgttggccg agtagccgat cagcagcatg acggcgaagg tgccgatgat cgataccggc
     6121 acggccacca acggaatgat cgtcgtcctg atcttgccga ggaacaggaa caccaccacg
     6181 ccgaccagca cgaaggcctc gcccaacgtg cggatcacct cggtgatggt cgcgttgacg
     6241 aagaccgtgg tgtcgtagaa cacgcgatag gagagatcct gcgggaagcg ttgcgccagc
     6301 gcgtccatga cgttgcgcac gtcgcgcgcc acctcgacgg cgttggcgcc cggcagctgg
     6361 tagatgccga tcgcggccgt cggtgcgccg ttgaaccggc tgtagcggtc cgacgacttg
     6421 gcgcccatgt cgacgcgcgc gacgtccttc acccgcacca ccgagccgtt gggattggcg
     6481 cgcagcacga tctcggcgaa ttcctcgggc cgggtgagcc ggcccttggt cttgatggtg
     6541 agctggaatt gctgatcgct ggtgactggc gcggcgccga tgcggccgag cgccgcctgc
     6601 acgttctgat tctgcacggc ggtgatcacg tcattcggcg tcagattgag cgccgtgagc
     6661 ttgtcgggat cgagccagac gcggagcgag tagtcgagcg gtccgaactg cgaggcctgg
     6721 cccacgccct tgatgcgcga cagcgcatcg atgacgttga tcgtcccgta gttgctgaga
     6781 tagagcgaat cgaaggtgcc cttgggcgaa tagagcagga cgacctgcag cagcgccgcg
     6841 gatttcttgc ggacgctgag cccctggcgc gacacttcgt ccggcagctg cggcaccgcg
     6901 agcgatgtcc ggttctgcac attgacggtg tcgatatcgg ggtcggtgcc gagcgcgaag
     6961 gtgacgttga gggtgtagct gccatcggcg ccgctggtcg actggtaata gagcgcgttg
     7021 tcgacgccgt tgatctgctg ctcgatcggc tgcgcgacgg tcgtttccac cacttccgcg
     7081 tcggcgcccg gataggtcgc ggtcaccgtc acctgcggcg ggacgatgtc ggggaactgc
     7141 gccaccggga tcgccgcgat ggcgaggagg cctgccagcg tgatgacgat ggagatgacg
     7201 aaggcgagcc gcggccgatc gatgaagatg gcggagatca aggggcgcct cacccgccct
     7261 tggtgacgac gggcgtggcc tgcacgacct gccccggccg cactttctgc acgccctcga
     7321 cgatgacccg ctctccctct ttcagccctg attcgacgac gatgtcggcg tgctcttccg
     7381 gacccgtcgc gacgcggcgg atctccgctt tctcatcgcc gtcgaccacc agcacgtagc
     7441 tgccggcctg gtcggccagg agtgccgctt gcgggatgac gaggcgcggc tgctcggcgc
     7501 gctcgcgcac cagcacggtc acgaattgtc cgtcgaccag cagccgctcg ggattgggca
     7561 ccgtgccgcg cagcgtcacc gtgtcggtct gctgatcgac ctgcggatcg gtgtaattcc
     7621 atgcgcccgg gtgctggtac tcgcgcccgt cggggagccg cagcacgatc tcgagctgcg
     7681 ccgcgaaggc gcgggcgtcc tggccgctcg ccgccgcgcg cgcctcctcc gcctgccgca
     7741 cgctcaccgg gaacagcaca tagatcgggt cctggctgac gatggtggcg agcacgccgc
     7801 tcgcggggtt gacgagattg ccctgcgtaa agcccgtgcg cccgatgcgg ccctggaccg
     7861 gcgccttgat ggtggtgtag ccgagattgg tctgcgccag cgtcaccgcg gcctcgttct
     7921 gcagcacgct ggcccgtgcc ccgtcgaggt tcgccttgtc ctggtcgacc gtcgcctggg
     7981 agatgttctg gcgcttcacg agctcggcga cgcggttgta ttgcagttcg gcgttgagcg
     8041 cctgtgaccg cgccgccgcc agattggcct ttgcctgctc gagctgcgcc tcgtattgca
     8101 ccgtctcgat ctggtagagc agttgccccg gcttcacttc gtcgccttcg gtgaacgcaa
     8161 ccttgtcgag gaagccctcg acccgcgcgc gcagctgcac gatatcgatc gccttgatcc
     8221 gcccgacgaa ctcggcggaa cggctcaccc ccttcatcat cgccggctgg acgccgacgg
     8281 ccggcggagg cgccggcgcc tgagccacgc cgctccggtt gcaggcggcg agcccgccca
     8341 gcagcaggac cacagcggcg cgcaacagcc tcgcgcggcc gcgatgagct cccgcgccgc
     8401 gctgcgacca tcggttcttg cgaccgccat gcattgccgg cctccgtgca atattcatgc
     8461 gcctgacgct cgatcgagcg aatgctagca cggtcgatat gccgatgccg agagcgccga
     8521 cggcgcgtcc ggcctcaccc cttggcgcgc ttgccgacga cggccgaagc accgagcagg
     8581 cggatgatct cttgggcaat ctcgccgggc ggcggccccg catcgaccgt cagggcgtcc
     8641 tcgtccggag acggttcttc gagcgtgtcg agctggttct gcagcaagct cgccggcatg
     8701 aaatggccgt gccgcccggc caggcgctcg gcgatgagcg cgcggctgcc gcgcagatag
     8761 atcagccgcg cttctggccg gtcgccgatg atgagcgcac gataggcgcg cttcagcgcg
     8821 gagcaggtga tgattcccgg ctgtttcaac gcgcgttgat gatcgatcca cgccgccacc
     8881 gcctcgagcc agggctggcg gtcggcgtcg gtgagcggaa tcccggcatg catcttggcg
     8941 acattcgctt cggggtggag cgcgtcccct tcctcgaagg gccagccgag gcgggcggcg
     9001 agggcgttgg cgaccgtgct cttgcccacc cccgacacgc ccatgacgac gaggatcggc
     9061 ggccagccgc gatggcggcg cgggctcgcg aggctgcgca cgccgtcctc gtcggcgacg
     9121 aagaccgtat cggcgcggct gatgaaaagc ccgcattcga cgacgccgac gacggcgcgg
     9181 atgcgtgtct cgagccgcgc cggatcggcg atcgggccga agcggcagtc gaggatccga
     9241 ttgccgctgt cggtggcgaa gggcgcgccc tctcgcgtcc ggcgcagagc gacggccgca
     9301 ccgagcccct cgagcgcggc cgcggtgact tcgagtccga aggcgaccac ttcgacggga
     9361 accggcacat gggtccccag ccggtccgcg agcttgccga catcgacgat gatgacgagg
     9421 cgccggctgg cggcggccgc gatcttctcg cgcagcaagg cgccgcccag ccccttcacg
     9481 agattgagcg tgccgcgctc cacctcgtcg gcgccgtcga tggtgagatc gatctgccga
     9541 tgctcggcga aagacgtgag cggaataccc aaggcccgcg cctgggcggc gatgcgctcg
     9601 gaggtgggaa tgccgaggaa gcgcaatccg tcgcgatggc gccgcgccag cgcctcgacc
     9661 gcgaaggcgg ccgtcgtgcc cgtgccgagg ccgaccacca tgccgtcttc gaccagctgc
     9721 accgccgcct ctgccgccgc gcgtttctgc gcgtcgcgct cggccgcgcc cggtgccgcc
     9781 gcgctcatga ttgctgcgcg ccggccgacg cgctggcgcg gatcgcggcg atcgccgcct
     9841 tgtagtccgg ggcgccgaag atcgccgagc cggcgacgat cgcattggcg cccgctgcgg
     9901 ccgcctgtcg cgccgtcgcg acgtcctcgc cgccatcgac ttcgatcacc ggatcgagcc
     9961 cgcgctcccg gcacatcgcg cggatgcgcc ggatctttgg cagcatctcc ggcaggaacg
    10021 cctgtccgcc gaatccgggg ttgaccgtca tcaccaggat gatgtccgca agatgcagca
    10081 catattcgat gagctcgggc gggctcgccg gatcgacgac cacgcccgct ttcttgccga
    10141 gcgcgcggat ctccgtcagc acgcgatgga gatgcgtcgt cgacccgggc tcggcctgga
    10201 ccagcaggtg atcggcgccg gccttggcga agcgggtgag atagcgctcg ggctcgacga
    10261 tcatgaggtg cacgttgagc ggtttcgcgg tcgagcggcg gatcgcctcc accacggtgg
    10321 gcccgatgga gatgttgggc acgaaacggc cgtccatgac gtcgacatgg atccagtcgg
    10381 cgccggccgc gtcgaccgcg cgcacctcgt cgcccagccg cgcgaaatcc gccgacagca
    10441 ccgagggcgc gatgagcacc ttcatggtca cgccgcgtcg ccggccgctg cccggtcgac
    10501 gaaccagaag agcgcgccga ccggccgcac gcgggcggcc ggcacctggc tcccgccggc
    10561 acggatggcg cgcaggatcg gcgccttctc ccggccagcc acgaggaaag cgacggcccg
    10621 gctgctctcg atcgcaggat aggtcatggt gatgcggatc tccggccggc catgggcgac
    10681 ggcggcgacc cagcgtccgc gctcttccag gaccggctct cccggcaaca gcgaggcggt
    10741 atggccgtct ggccccagcc ccagcaaggt gatgtcgaag agcggccgcg ccggatccag
    10801 gacgcggccg ccataagcgt cctgcaagat ccgttcatag cgccgcgccg cgtcgtcggg
    10861 cgtgccgtcg accgggacgg gatggatgtt ctcgggcggg atcggcgctt tggacagcat
    10921 cgccgcgcgc gccatccgat agttgctgtc ggggtgatcc tggggcacga agcgctcgtc
    10981 cccccaatac cacgagacac gcggccacgg gaaccggctc ttgaacgccg gcgacgccag
    11041 cagcgcgtaa agcgtctcgg gcgtcgatcc gcccgacagc gacacccgaa acgggcgctc
    11101 cgccgcgagc gcggcgcgcg tcatccattc cgccacatgc cgcgccagcg ccgcaggatc
    11161 ggcaaatacc tccaccttct ccgtcggggc gaggcggtcc tttgacgtca cgggcgtgca
    11221 tcccgtacac gcggagggca attgcatatg ccaccgagac gcagatagga aaagttcacc
    11281 acggaggcac ggagggcacg gagaagcgaa gaatggcgcg cttcgcgcgc cgaacccttc
    11341 ttctccgtgt cctccgtgcc tccgtggtga aaccgatatg cgatcgccct gcgttcacgc
    11401 ggaaccgttc ttccctcgtc gtccccgcga aagcggggac ccaggagtcg ccggcacgtc
    11461 gctcgcccct ggattcccgc tttcgcggga atgacgactt tgaagagcag tgtcgattcc
    11521 acggccatgc gctccatgtc aacccaagcg accgaggacg aagcgctcca tcgccttggc
    11581 gaacccctcg tcgttgtagg aatcggtggt ggcgctggct tgcgccttca cctggtcgga
    11641 ggcgttgccc atggcaatgc tgaagcccga gcgcttgaac atcagcacgt cgttgggctg
    11701 gtcgccgatg gtggcgatct cttcgggggg cacctcgaga tgctgcgaga gataggccac
    11761 gaccgcgcct ttgttggcgt ccttgctggt gacgtcgaga taatagggct gcgaccggct
    11821 cgccgtcgcg cgctcgccga aagccgcctg ggcgtcggcc tcgcagcgcc gcacgcaatc
    11881 gaggtcgtcg ctgatgccga cgatcttcgc ggcgccgtcg agcatgtcgt cgaaatcggc
    11941 gacgattttg ggctcgaact tgaccgtcca tgcctcgcgc gcgacatgcg gcgcgccggg
    12001 cttggtgatc agccagtcat tgccgtgata gacccaggca tcgaggccat gatggcgaat
    12061 gaggtcgatg gcctggcgcg cgacatcggc cggcagcgtg ttctcggcga ggatcgaaag
    12121 gtcccgcttc acgaagatcc cgccattgaa gccggcgacc ggcgtcgtga gctcgagcgc
    12181 gtcgaacagc atcgccatgc cgcggggcgg ccgaccgctg gtgatggcga agcggatgtc
    12241 cgcctcgccg agtgccttca cggccgcctg cgcgcgcggc gtcaggacct tgtcctccgt
    12301 gacgagggtg ccgtcgacat cggcgagcac cagggagatc ttgcgtttct cggccattgc
    12361 gcgcgctccg cctcagccga gcggccgcca ggcgtgcccg tcttgcgcca gcagcgcgtc
    12421 ggccgcttcc ggcccgctgc tgcccgcagc gtagttcggg aagtccttcg gcgcgttttc
    12481 tgcccaggca tcgaggatcg gctgcaccgc ctgccagccc gactcgatgt tgtcggcgcg
    12541 ctggaagagc gtcgcgtcgc
//
LOCUS       DASYNS010000446         7110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5106602, whole genome shotgun sequence.
ACCESSION   DASYNS010000446 DASYNS010000000
VERSION     DASYNS010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7110
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5106602"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VGP50_13940"
     CDS             complement(<1..1125)
                     /locus_tag="VGP50_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV8028530.1"
                     /translation="MIPAVLPTYARNELTFERGEGAYLYATNGKKYLDFASGVAVTAL
                     GHCHPHLVKALTEQAQRLWHCSNLFQIEGQKRLAQRLVDATFADTVFFCNSGAEAVEC
                     AIKMARKFHAETGHPEKYRLIACTGSFHGRTLATLAAAGNEKYLTGFGPPAPGFDHVA
                     FGNLNEMRAAITHETAGILVEPVQGEGGLATAPEGYLAGLRAICDEFGLLLVYDEVQC
                     GMGRTGKLFAHEWADAPPDILATAKGLGGGFPVGACLATERAAVGMKAGSHGSTFGGN
                     PLAMAVGNAVLDVLLSDGFLANVERMARLLHRRLSAIAAEYPEVVAEIRGKGLLAGFK
                     CAVPNTEMVDRLRAGGLLAVGAGDNAVRLLPPLVIGESHVD"
     gene            complement(1391..2194)
                     /locus_tag="VGP50_13945"
     CDS             complement(1391..2194)
                     /locus_tag="VGP50_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8028531.1"
                     /translation="MHPDLTPRLRRFGAVNWLGFWTLYVREVRRFLKVSMQTIAAPVV
                     TTLLFLAVFLLALGGQQRHVGDISYVEFLTPGLIMMAMAQNAFANTSSSLMIAKVQGN
                     IVDVLMPPLSPGELTWGFALGGVTRGLMVGIVVAAAMALFVPLHFAAPGFALFHAIAA
                     SLLLSLLGLLGGIWADKFDHIAALTNFVVTPLSFLSGTFYSIERLPEPWHQVALFNPF
                     FYMIDGFRYGFIGHADGPVWLGVAVLIVINAALLLLCHSWFARGYKLKA"
     gene            2299..2910
                     /locus_tag="VGP50_13950"
     CDS             2299..2910
                     /locus_tag="VGP50_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEV8028532.1"
                     /translation="MAEDREEERADSATRRRRRGPRTVTAPLLEKAALHYLERFASSS
                     AHLKRVLMRRVARSARADGSDAAAGERLVDDIVQRYLRAGLLDDAAYAASQAASLRRR
                     GASRHAIRGKLALKGIDAAQVEHALASVEGTEGSEIAAAAAFARRRRLGPYRAGATRD
                     SHRQKDLATLARAGFSLDLARRVLAAADPDALDALVRGAADEN"
     gene            complement(2916..5138)
                     /locus_tag="VGP50_13955"
     CDS             complement(2916..5138)
                     /locus_tag="VGP50_13955"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEV8028533.1"
                     /translation="MICSSILRALAVVACLLVPSAMPASAQSPQSSAPAAAPPSPEEL
                     ERLVNTLQDDQARAKLVEQLRGLIAAQRGVEETQAEENPATLLNDLSAEIDAISGEIL
                     AAAAVILDAPRLVSWVQAQATRPEGRALWFDVGVKLVVIFGTAVLVEWLLRLLLRRPA
                     ARLGTRSSDSTAVQVFLLLLQFVLEALPIGAFAVVAYSVLPLVHPRKPTLLTAQVIIH
                     AVVTARLILAAARVALVSPGAATLHALGEETRNYLYIWARRFTSWAVYGFALAEATWW
                     LGVPGAIYTLLLRGTLLVLGGLAIVFILQNRAAMAELLRGKLGADGRPNGRGWRLLRQ
                     RLADAWHVVAITYVVGIFGTYVLRIRGGGVFVFRATVLSVVVLVAAGLIVHFVRRSSQ
                     RGFAIRADLKARFPTLEARANRYLPALTIATATVVYFFAALALLQAWGIDAFAWFTTT
                     FGRKIAGSLLSIVAVLVVSLVIWELFGSAIERYMNHIDADGRPVSRSARARTLLPLFR
                     TAVFILLFTVVTLIVLSELGLDIAPLIAGAGVVGIAIGFGSQALVKDVITGLFILLED
                     TLAVGEVVDVGKNHIGSVEAITIRTIRLRDMSGTVHTIPFSEVSTVRNMTRDYAYFVA
                     DVGVVYREDPDHVVAVLRKVAADLAKDPAWAPSIAEPLEVIGVDRFTDSAVVIRVRLK
                     TVPLRQWPVGREFNRRMKKAFDAAGIEMPAANQARYLPEGDPAGVPATQGPEPVTVAT
                     "
     gene            complement(5226..6686)
                     /locus_tag="VGP50_13960"
     CDS             complement(5226..6686)
                     /locus_tag="VGP50_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV8028534.1"
                     /translation="MEITSLIDLRERVGRFFVTALWLHLPVLFVIGVINDTNWLAAIV
                     IGAAVSAIATVVWLGDKQGALGRYVITIALVTMVSLMVWLSAGELQQDVHMYYFTAYA
                     ALAAFCDWRVIVLGAAATALHHLGLNYILPYAVFRDGANLWRVLLHAGIVLTAAGVLI
                     WLTQNLSRLFGAGQAALDAMARANQRESELTAERLRTHHDMHAERRRLTIETADKFET
                     IVSSVVDRVAEAARSAQQTALRVVAAIGTNRVQSEAAAATLQETTGNVHMVAAAVETL
                     AATTEAIGRQVAQSAAISVKAVDEAARTDAAVQGLADAAQRIGEVVQLINDIASQTNL
                     LALNATIEAARAGDAGKGFAVVASEVKSLASQTAKATDDIGAQIAQIQGATRNAVAAI
                     RGIGETIGEISEISTSIAAAVVEQTAATRDIAGSVQQAAAGATEATRTVEAVSDTAKA
                     SGGSADELDGATDRLATLADTLRGEVGRFLGQVRAA"
     gene            complement(6722..7060)
                     /locus_tag="VGP50_13965"
     CDS             complement(6722..7060)
                     /locus_tag="VGP50_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028535.1"
                     /translation="MAGRRASGWPAALVAAALILASVLPIAWLEARPRNDRNVAVMFA
                     PWTTRGAVLARVTIAEGLIVREGIIDSIVVVHGGSAGLIDRLYAAGAWAVVDPVAFGG
                     CLVRSPRRTT"
BASE COUNT         1250 a         2357 c         2491 g         1012 t
ORIGIN      
        1 atccacatgg ctctcgccga tgacgagcgg cggcaagagc cgcaccgcgt tgtcgcccgc
       61 gcccaccgcg agcaagccgc ccgcgcgcaa gcggtcgacc atctcggtgt tgggcacggc
      121 gcatttgaag ccggcgagca gacccttgcc gcggatttcg gcaaccacct cgggatactc
      181 cgcggcgatg gcggagagcc ggcggtggag caggcgggcc atgcgctcga cattggcaag
      241 gaagccgtcg ctcaggagca cgtccagcac ggcgttcccc accgccatcg cgagcggatt
      301 gccgccgaag gtcgagccat gagagcccgc cttcatgccg accgcggcgc gctcggtggc
      361 aaggcaggcg ccgaccggga agccgccgcc cagccccttg gcggtggcga gaatgtcggg
      421 cggcgcgtcc gcccattcat gggcgaagag cttgccggtg cggcccatgc cgcattgcac
      481 ctcgtcatag acgaggagca ggccgaactc gtcgcagatc gcgcgcaggc cggcgagata
      541 gccttcgggc gcggtggcga gaccgccctc cccctgcacc ggctcgacga gaatgccggc
      601 cgtctcatgg gtgatggccg cgcgcatctc attcaaattg ccgaaggcga catggtcgaa
      661 gccgggggcc gggggcccga agcccgtcag gtatttctcg ttcccggcgg cggcgagcgt
      721 cgccagagtc cggccgtgga atgagccagt gcaggcgatc agccgatatt tctcgggatg
      781 gccggtctcg gcgtgaaact tgcgcgccat cttgatggcg cattcgacgg cctcggcgcc
      841 ggaattgcag aagaagacgg tgtcggcgaa agtggcgtcg acgagacgct gggcgaggcg
      901 cttctggccc tcgatctgga aaaggttgga gcagtgccag agacgctgcg cctgctcggt
      961 caaggccttg acgagatggg gatggcagtg accgagcgcg gtgacggcga cgccgctggc
     1021 gaaatcgagg tatttcttgc cgttggtggc ataaagatag gcgccttcgc cccgctcgaa
     1081 cgtcagttcg ttccgagcat aggtcggcaa aacggctgga atcacgggag tctctcctgc
     1141 gcgaatggga tggaaggggt caagggatcg gtcgagcgat cgcctaccct cgtcgccgtc
     1201 gcggcgccgt ccgccctttc ccagagagcc ggtcctgccg gcacgcttcg cgcatcatca
     1261 gccactccac ccctctccgg tccgccgcgg ttgcggattg accagcggga gaagcgggaa
     1321 agtatggtgg ccgtctaagc ggctgtcaat gttgggcttg cgacactttg gcggcagccg
     1381 tcgcgggtta tcacgccttg agcttgtagc cgcgcgcgaa ccagctgtgg cagagcagga
     1441 gcaacgcggc gttgatcacg atcagcacgg cgacgccgag ccagaccgga ccgtcggcat
     1501 ggccgatgaa gccgtagcgg aagccgtcga tcatgtagaa gaagggattg aacagcgcga
     1561 cctgatgcca gggctccggc agccgctcga tcgagtagaa agtgccggag aggaaagaga
     1621 gcggcgtcac cacgaaattg gtcagcgccg cgatgtgatc gaacttgtcg gcccagatgc
     1681 cgcccaagag gccgagcagc gacaacagca gcgaggcggc gatcgcgtgg aacagcgcga
     1741 agcccggcgc cgcgaaatgc agcggcacga agagcgccat ggccgcggcg acgacgatgc
     1801 ccaccatcag gccgcgcgtg acgccgccca aggcgaagcc ccaagtgagt tcgcccggac
     1861 tcaagggcgg catcaggacg tcgacgatgt tgccctgcac tttggcgatc atcagcgagg
     1921 acgacgtgtt ggcgaaggcg ttctgcgcca ttgccatcat gatgaggccg ggcgtcagga
     1981 actcgacata agagatgtcg ccgacatggc gctgctggcc gccgagcgcc aggagaaaga
     2041 cggcaaggaa caagagcgtc gtcaccaccg gggcggcgat ggtctgcatc gacactttga
     2101 ggaagcgccg gacctcgcgc acatagagcg tccagaagcc gagccagttg acggcgccga
     2161 accggcgcag acgcggcgtg agatcgggat gcatgagcaa tcctagaagg tgccgggccg
     2221 cgcgggcaag gagcgctgag cgctgcaatg ctgttgtgca ttgcagcgcc gtcgccacgc
     2281 atgctctaga atgacggcat ggccgaggat cgcgaggaag agcgcgccga cagcgcgaca
     2341 cggcgccgac ggcgcgggcc gcgcacggtg accgcgccgc tgctcgagaa agcggcgctc
     2401 cattatctcg agcgcttcgc aagctcgagc gcgcatctga agcgcgtgct gatgcgccgg
     2461 gtcgcgcgct cggcgcgcgc cgatggcagt gacgcggcgg cgggcgagcg cctggtcgac
     2521 gacatcgtcc agcgctatct gcgcgccggc ctcctcgatg acgcggctta tgccgcgagc
     2581 caagcggcga gtctgcgccg gcgcggcgcc tcgcgccatg ccatccgcgg caagctcgcc
     2641 ttgaagggca tcgatgcggc gcaggtcgag cacgcgctgg cgagcgtcga aggcacggaa
     2701 gggagcgaga tcgccgccgc ggcggccttc gcgcgacggc ggcggctcgg cccctatcgc
     2761 gccggcgcga cgcgcgacag ccatcgccag aaggatctcg cgacgctggc gcgggccggc
     2821 ttcagcctcg atctggcgcg gcgcgtcctg gcggcggccg atccagacgc gctcgacgcc
     2881 ctggtgcgcg gcgccgcgga cgaaaactga taccgtcagg tcgcgacggt caccggctcc
     2941 ggtccctggg tggccggaac gccggcgggg tcgccctcgg gcaagtagcg ggcctgattg
     3001 gcggcgggca tctcgatgcc ggcggcatcg aaggccttct tcatgcgacg gttgaactcg
     3061 cgcccgaccg gccattgcct gagcggcact gtcttgaggc gaacgcggat caccacggcg
     3121 ctgtcggtga aacggtcgac gccgatgact tcgagcggct cggcgatcga gggcgcccag
     3181 gccgggtcct tggcgagatc ggcggcgacc ttgcgcagca ccgcgacgac atggtcggga
     3241 tcctcgcggt agacgacgcc gacatcggcg acgaaatagg cgtagtcgcg agtcatgttg
     3301 cgcaccgtcg acacctcgga gaagggaatg gtgtggaccg tgccggacat gtcgcggagc
     3361 cggatggtgc gaatggtgat ggcctcgacc gagccgatat ggttcttgcc gacgtctacg
     3421 acttcgccga cggcgagcgt gtcttcgagc aggatgaaga ggccggtgat gacgtccttg
     3481 accagcgcct gagagccgaa gccgatggcg atgccgacga cgccggcgcc ggcgatcagc
     3541 ggcgcgatgt cgagaccgag ctcggagagc acgatcagcg tcacgacggt gaagagcagg
     3601 atgaagacgg cggtgcggaa cagcggcagc aacgtgcggg cgcgcgcact gcgcgatacg
     3661 ggccggccgt ccgcatcgat gtggttcata taacgctcga tcgccgagcc gaagagctcc
     3721 cagatcacga gcgagacgac cagcacggcg acgatcgaca gcaggctgcc ggcgattttc
     3781 cgcccgaagg tcgtggtgaa ccaggcgaag gcatcgatgc cccaggcctg cagcaaagcg
     3841 agcgcggcga agaaatagac gacggtcgcg gtcgcgatgg tgagcgccgg cagatagcgg
     3901 ttggcgcgcg cctcgagcgt gggaaagcgc gccttgagat cggcgcggat ggcgaagccg
     3961 cgctggctcg agcgccgcac gaaatggacg atcaagccgg ccgcgaccag cacgacgacg
     4021 ctcaacaccg tggcgcggaa cacgaacacg ccgccgcccc ggatgcgcag cacataggtg
     4081 ccgaagatgc cgacgacgta ggtgatcgcc acgacgtgcc aggcatcggc cagccgctgc
     4141 cgcagcagcc gccagcctct gccattcggc cggccgtcgg cgccgagctt gccgcgcagc
     4201 agctccgcca tcgccgcgcg attctgcagg atgaagacga tcgcgaggcc gcccagcacc
     4261 aggagcgtgc cgcgcagcag cagggtatag atcgcgcccg gcacgcccag ccaccaggtt
     4321 gcctcggcca aggcgaagcc atagaccgcc cagctggtaa agcgccgcgc ccagatataa
     4381 aggtaattgc gcgtttcctc gccgagcgca tggagcgttg cggcgcccgg cgacaccaaa
     4441 gcgacgcgcg cggcagccag gatcagccgc gccgtgacca cggcgtggat gatcacctgg
     4501 gcggtgagga gcgtcggctt gcgcgggtgc accagcggca ggacggaata ggcgaccacg
     4561 gcaaaggccc cgatcggcaa cgcctcgagg acgaattgca gcaggagcaa gaagacttgc
     4621 accgccgtcg agtcgctgga ccgcgtgccg agacgcgccg ccggccgccg gagcagcagc
     4681 cgcagcagcc attcgacgag caccgccgtg ccgaagatga cgacgagctt caccccgaca
     4741 tcgaaccaga gcgcccgccc ttcgggacgg gtcgcttgcg cctggaccca ggagacgagg
     4801 cgcggcgcat cgaggatgac cgcggctgcc gccaggatct cgccgctgat ggcatcgatc
     4861 tccgccgaga gatcgttgag cagcgtcgcc gggttttcct cggcctgcgt ctcttcgacg
     4921 ccgcgttgcg cggcaatgag gccgcggagc tgctcgacga gcttggcgcg cgcctgatcg
     4981 tcctgaagcg tgttgacgag gcgctcgagc tcctcgggtg agggaggcgc agcggccggc
     5041 gccgaagatt gcggcgattg cgcgctggcc ggcatggcgg agggaacgag caggcatgcc
     5101 acgaccgcaa gcgcgcgcag gatgctcgag cagatcatgg cgcgggagat taaccgcccc
     5161 ttcgcacagc gcaagcattc tgcggcggca gcgcaaaatt gccgcgcgcc gcgcgccggc
     5221 cttcctcagg cggcgcgcac ctggccgagg aagcggccga cctcgccgcg caaggtatcg
     5281 gcgagcgtcg cgagccggtc cgtggcccca tcgagctcgt ccgccgagcc gccgctcgcc
     5341 ttcgcggtgt cgctcaccgc ctccaccgtg cgcgtcgcct cggtggcgcc ggccgccgcc
     5401 tgctggacgc tgccggcaat gtcccgggtt gccgcggtct gctccacgac ggccgctgcg
     5461 atcgatgtcg agatctcgct gatttcgccg atggtctcgc caatgccgcg gatcgcggcg
     5521 acggcgttgc gcgtcgcacc ctggatttgc gcgatctgag caccgatgtc gtcggttgcc
     5581 ttcgccgtct gactggcgag cgacttgacc tccgaggcga cgaccgcgaa gcctttgccc
     5641 gcgtcgccgg cgcgcgccgc ctcgatcgtg gcgttgagcg ccaggaggtt ggtctggctc
     5701 gcgatgtcat tgatgagctg caccacttcg ccgatgcgct gcgccgcatc cgcgagcccc
     5761 tggacggcag cgtcggtgcg cgccgcttcg tcgacggcct tcaccgagat cgccgccgat
     5821 tgagccacct gccggccgat cgcctcggtc gtggcggcca gcgtctcgac tgccgccgcc
     5881 accatgtgga cattgcccgt cgtctcctgc aacgtcgcgg ccgcggcttc cgactggacg
     5941 cgattggtcc cgatggcggc gaccacgcga agcgccgtct gctgcgcaga gcgcgcggct
     6001 tcggcgaccc ggtcgacgac cgagctcacg atcgtctcga acttgtcggc ggtctcgatc
     6061 gtgaggcgcc ggcgctccgc gtgcatgtca tgatgcgtgc gcaagcgctc ggccgtgagc
     6121 tcgctctccc gctggttcgc ccgcgccatg gcgtcgagcg ccgcctgacc ggcgccgaaa
     6181 agccgcgaca ggttctgcgt gagccagatg aggacgcccg cggcggtgag gacgatgccg
     6241 gcgtggagca gaacgcgcca gagattggcg ccatcgcgga acacggcata aggcaggata
     6301 tagttgaggc cgagatggtg gagcgccgtc gccgccgcgc cgagcacgat gacgcgccag
     6361 tcgcaaaacg ccgcgagtgc ggcataggcg gtgaagtagt acatatgcac gtcctgctgc
     6421 agctctcccg cggagagcca caccatcagc gacaccatcg tgacgagggc gatggttatg
     6481 acatagcgtc cgagcgcgcc ctgcttgtcg ccgagccaga cgactgtggc gattgccgac
     6541 accgccgcgc cgatcacgat cgccgccagc cagttcgtgt cgttgatcac gccaatgacg
     6601 aacagtaccg gcagatgcag ccagagcgcc gtcacgaaaa agcgcccgac ccgttcgcgt
     6661 agatcgatga ggctggtgat ctccatggct tcctctgtct ctgtctgact catatgttca
     6721 ttcacgttgt ccggcgcggc gacctcacga gacagccgcc gaacgccacc ggatcgacga
     6781 cggcccacgc gccggcggca tagagccggt cgatcaaccc ggcgctgccg ccatgaacca
     6841 cgacgatgct gtcgatgatg ccctcgcgga cgatgaggcc ttccgcgatg gtcactcgcg
     6901 cgagcacagc gccgcgagtc gtccatggcg caaacatcac cgccacgttc cgatcatttc
     6961 gcggccgcgc ctcgagccag gcgatcggca agacgcttgc cagaatcagc gcggccgcca
     7021 cgagggcggc cggccatccg ctcgcgcgcc tgccggccac gacttttccc ttcgatcagc
     7081 gagaaccctg cactcggatt atcgcaaacc
//
LOCUS       DASYNS010000447         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4486084, whole genome shotgun sequence.
ACCESSION   DASYNS010000447 DASYNS010000000
VERSION     DASYNS010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4486084"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VGP50_13970"
     CDS             complement(<1..797)
                     /locus_tag="VGP50_13970"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEV8028536.1"
                     /translation="MFAAISRYETRSETPVPLMLERGDGAAEIVFARRAAGRTALAHL
                     YQRSPCRVLFPAPEPGDLSLAALLTTSGGLTGGDRLRLAVTVEAGAAALATTAAAEKV
                     YRSAEGDVEIAVALTVDERGWLEWLPQETILFDGACLDRRLVASVAPAARFLGAETLV
                     FGRAAHGERFARGRLHESWRVRFGGRLVWADTLGLAGDVAAQLGAPAGFAGAAALATA
                     VYVGADAAAHLALARALAEGGDCRGGATLLGPVLVARFLDARAEIVRT"
     gene            complement(772..1467)
                     /gene="urtE"
                     /locus_tag="VGP50_13975"
     CDS             complement(772..1467)
                     /gene="urtE"
                     /locus_tag="VGP50_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEV8028537.1"
                     /translation="MLAAENIDLYYGASHILRHVGIVAEKGTVTCVLGRNGVGKTSLM
                     RAIIGLQPIRAGRILWEGEDITRLKPHERARRGIALVPQGREIFPRLSVEENLETGFA
                     PLPRAQRRVPEEIFTLFPVLRGMLKRRGGDLSGGQQQQLAIARALVMRPQLLILDEPT
                     EGIQPSIIKDIERVIRGLAARGDMAILLVEQYFEFARALAQRYVVMERGEVVLSGDAR
                     DMVEADVRRYLTV"
     gene            complement(1616..2383)
                     /gene="urtD"
                     /locus_tag="VGP50_13980"
     CDS             complement(1616..2383)
                     /gene="urtD"
                     /locus_tag="VGP50_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEV8028538.1"
                     /translation="MTPAASPATVTEALLYLDGVTVSFDGFKALNGLSLVVDRGELRS
                     IIGPNGAGKTTMMDVITGKTRPDTGTVFFDGSHDLTRLDEATIANLGIGRKFQKPTVF
                     ENLTVFDNLELAQKSPRDMGHVLFAKLGGAERDRIAETLRMIGLAGRERSIAGSLSHG
                     QKQWLEIGMLLIQDQRLLLLDEPVAGMTDRETEATAALISSLAGTHTVIVVEHDMEFV
                     RSLGTRVTVLHEGSVLADGSIDFVQSQPAVVEVYLGR"
     gene            complement(2380..>3010)
                     /locus_tag="VGP50_13985"
     CDS             complement(2380..>3010)
                     /locus_tag="VGP50_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEV8028539.1"
                     /translation="LTYALWLAFFRNDMGFGGNNGLTDFKDLLGFDLHAPATRRGLYV
                     ASVVALGAGYLLCRFITRSKFGRLLLALRDAESRVRFLGYSVERAKLFTFTLSAVLAG
                     IAGALYVPQVGIINPSEFSPGNSIEVAIWVAVGGRGTLLGAIIGAVVVNFAKTWLTGS
                     LPEAWLFVLGGLFVLVTLAIPRGLLGLGGQLRLGRRAQPQAVAGEEQSP"
BASE COUNT          504 a         1025 c         1057 g          424 t
ORIGIN      
        1 gtacggacaa tctcggcgcg cgcatcgagg aatcgcgcga cgagcacggg gccgagcagc
       61 gtcgcgccgc cgcgacagtc gccgccctcg gcgagcgcac gggccagcgc cagatgcgcg
      121 gccgcgtcgg cgccgacata gaccgcggtc gccagcgccg cggcgccggc gaaccccgcc
      181 ggcgcgccga gttgcgccgc gacatcgccc gcgaggccga gcgtgtccgc ccagacgagc
      241 cgcccgccga agcggacgcg ccaggactcg tggagccggc cccgcgcgaa tcgctcgcca
      301 tgcgcggcgc ggccgaacac cagcgtctcg gcaccgagga atcgcgcggc cggcgcgacc
      361 gaagccacga gtcgccgatc gaggcatgcg ccatcgaaga gaatggtttc ctgcggcagc
      421 cattcgagcc agccgcgttc gtcgaccgtc agcgcgaccg cgatttcgac atcgccctcg
      481 gccgagcggt agaccttctc ggccgccgcc gtcgtcgcga gcgccgcggc gcccgcttct
      541 accgtcaccg cgaggcgcaa gcggtcgcca ccggtaaggc cgcccgaggt cgtgagcagc
      601 gcggccagcg acaggtcccc gggctcgggc gccgggaaca ggacgcggca ggggctgcgc
      661 tgatagagat gggcgagagc ggtgcggccg gcggcgcgcc gcgcgaagac gatctcggcg
      721 gcgccgtcgc cgcgctcgag catcagaggt accggcgtct ccgagcgggt ctcataccgt
      781 gagatagcgg cgaacatcgg cttccaccat gtcgcgggcg tcgccggaca ataccacttc
      841 gccgcgctcc atgacgacat agcgctgggc gagcgcgcgg gcgaattcga aatactgctc
      901 gaccagcagg atcgccatgt cgccccgcgc cgcgagaccg cggatgacgc gctcgatgtc
      961 cttgatgatc gagggctgaa taccttccgt cggctcgtcg agaataagga gctgcggccg
     1021 catcaccagc gcgcgggcga tggcgagctg ctgctgctgc ccgcccgaga gatcgccacc
     1081 ccggcgcttc agcatgccgc gcagcaccgg gaagagcgtg aaaatctcct cgggaacgcg
     1141 gcgctgcgcc cgcggcagcg gcgcgaagcc ggtctcgaga ttctcttcga cgctcaggcg
     1201 cggaaagatc tcacggccct gcggcacgag agcgatgccg cggcgcgcgc gctcatgcgg
     1261 cttcagtctc gtgatgtcct cgccctccca gaggatgcgg ccggcgcgga tcggctggag
     1321 gccgatgatc gcccgcatca ggctcgtctt gccgacgccg ttgcgcccga ggacgcaggt
     1381 caccgttccc ttctcggcga cgatgccgac atggcgcagg atgtggctgg cgccgtagta
     1441 gagatcgatg ttttcggcag cgagcatccc tcacccttcc cgtgcgcgcg acagaagatt
     1501 caccacagag gcacagagac acagagaaga aaagatgttt ggcgcgcgaa gcgcgccagt
     1561 ctcctcttct ctgtgtctct gtgcctctgt ggtgaatatc aacggcgcag ctccttcatc
     1621 ggccgagata cacttccacc acggcgggct ggctctgcac gaagtcgatc gagccgtcgg
     1681 ccagcaccga gccttcgtgg agcacggtca cgcgcgtgcc gaggctgcgc acgaactcca
     1741 tgtcgtgctc gacgacgatc acggtgtgcg tccccgcgag cgagctgatc agcgccgccg
     1801 tcgcctcggt ctcgcggtcg gtcatgccgg cgacgggctc gtcgagcagc aagaggcgct
     1861 ggtcctggat cagcagcatg ccgatctcga gccattgctt ctggccgtgg ctcaagcttc
     1921 ccgcaatgct gcgctcgcgg ccggcgaggc cgatcatgcg caaggtctcg gcgatgcggt
     1981 cgcgctcggc gccgccgagc ttggcgaaga ggacgtggcc catgtcgcgc gggctcttct
     2041 gagccagctc gaggttgtcg aagacggtga ggttctcgaa cacggtgggc ttctggaatt
     2101 tgcggccgat gccgagattg gcgatcgtcg cctcgtcgag ccgcgtcagg tcgtggctac
     2161 cgtcgaagaa gaccgtgccc gtatcgggcc gggtcttgcc ggtgatgacg tccatcatcg
     2221 tcgtcttgcc ggcgccgttg gggccgatga tgctccggag ctcgccgcga tcgacgacga
     2281 gcgagagccc gttcaatgcc ttgaacccgt cgaagctcac ggtgacgccg tcgagataga
     2341 ggagcgcctc cgtgacggtg gccggcgatg cggcgggggt catggcgact gctcctcgcc
     2401 ggcgaccgcc tgcggctgcg cgcggcgtcc gagccgcagc tgtccgccga ggccgaggag
     2461 gccgcgcggg atggccagcg tcaccaggac gaaaaggccg cccagcacga agagccaggc
     2521 ctccggcagc gagcccgtga gccaggtctt ggcgaaattg acgacgacgg cgccgatgat
     2581 ggcgcccaag agcgtgccgc gcccgccgac ggcgacccag atggcgacct cgatggagtt
     2641 gccgggcgag aattcgctgg ggttgatgat gccgacctgc ggcacataga gcgcgcccgc
     2701 aatgccggcc agcaccgccg agagcgtgaa agtgaagagc ttcgcccgct cgaccgaata
     2761 tccgaggaag cgcacgcggc tctcggcatc gcgcaaggcc agcagcagcc ggccgaattt
     2821 cgagcgcgtg atgaagcggc agagcagata gcccgcgccc agcgcgacca ccgacgcgac
     2881 atagaggccg cgccgcgtcg ccggcgcatg caggtcgaag cccaagagat ccttgaaatc
     2941 ggtgaggccg ttgttgccgc cgaagcccat gtcgttgcgg aagaaggcga gccagagcgc
     3001 gtaggtgagc
//
LOCUS       DASYNS010000448         2977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2455959, whole genome shotgun sequence.
ACCESSION   DASYNS010000448 DASYNS010000000
VERSION     DASYNS010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2455959"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VGP50_13990"
     CDS             complement(<1..663)
                     /locus_tag="VGP50_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEV8028540.1"
                     /translation="MNSTSSLVDRAGLDRTRLAKVISRGLEGADDGELFLEYRQAEML
                     LFDNGRLKQATYDTSQGFGLRAVKDEAVGYAHASDVSEAAIARAADAVRAVKGGYSGD
                     YAGPPPRSNVKLYSEESPLASPAFEAKARLLETIDAYARAKDPRVRQVSVSLGASWQA
                     IEIVRGDGETYRDIRPLVRVNVSVVVGDGDRQEVGSAGYGGREGYERFITADNWQGAV
                     DNA"
     gene            complement(831..2120)
                     /gene="murA"
                     /locus_tag="VGP50_13995"
     CDS             complement(831..2120)
                     /gene="murA"
                     /locus_tag="VGP50_13995"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV8028541.1"
                     /translation="MDRIRIVGGRRLTGTIPISGAKNATLPLMIASLLTEETLILDNV
                     PRLADVVLLQRILGNHGVDVMVSGKRPGETADHGQTLHVSAANIVDTTAPYELVSKMR
                     ASFWVIAPLVARMGRARVSMPGGCAIGTRPVDLLIMALAELGADIEIDSGYVVAAAPN
                     GLKGGKIVFPKVTVGGTHTAVMAASLAKGTTVIENAACEPEIVDVADCLNKMGAHISG
                     AGTQRIIVEGVAKLGGARHAVLPDRIETGTYAMAAAMAGGDIFLQNARAELLQAALDV
                     LTQTGVTVSSTNQGIRIARDGADLVPIEVATAPFPAFPTDLQAQLMALMTRARGTSHV
                     VETIFENRFMHVQELVRLGARIQLDGERATIEGVERLTGAPVMATDLRASVSLVIAAL
                     AAEGETIVNRVYHLDRGFERLEDKLARCGADIERLSG"
     gene            complement(2156..2344)
                     /locus_tag="VGP50_14000"
     CDS             complement(2156..2344)
                     /locus_tag="VGP50_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028542.1"
                     /translation="MISRGKELKQQARSKRLSAALRENLKRRKAQARGRAASPSGDEA
                     APARHAQSPDFRRNRGSD"
     gene            complement(2359..>2977)
                     /locus_tag="VGP50_14005"
     CDS             complement(2359..>2977)
                     /locus_tag="VGP50_14005"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEV8028543.1"
                     /translation="AGANIMAEAHEQMEQAEHVEHASHENRRIALLIAVLALFLSFSE
                     TLGKSAQTEAISANVESSNLWAFFQAKTVRMTLVKTAAEELKVTAAAAADPAAKAAID
                     KQIEAWQKTAARYDSEPETHEGRKELSERAKEAEEGRDLAMAKYHHYELASAAFQIGI
                     VLASAAVITSAIALVWLSGALGILGLAILALGVYAPHALDLGHLL"
BASE COUNT          447 a         1008 c         1006 g          516 t
ORIGIN      
        1 ggcgttgtcg accgcgcctt gccagttatc ggcggtaatg aagcgctcat agccctcgcg
       61 gccgccataa ccggcgctgc cgacttcctg gcggtcgccg tcaccgacca cgaccgagac
      121 attgacgcgc accagcgggc ggatgtcgcg ataggtttcg ccgtcgccgc gcacgatctc
      181 gatcgcctgc cagctggcgc cgaggctgac cgagacctgg cgcacccgcg gatccttggc
      241 gcgcgcgtaa gcgtcgatgg tttcgagaag ccgcgccttg gcctcgaaag cgggactcgc
      301 gagcgggctc tcctcgctgt agagcttgac gttgctgcgc ggcggcgggc cggcgtagtc
      361 gccggaatag ccgcccttga ccgcgcgcac ggcgtcggcc gcccgcgcga tcgccgcctc
      421 cgacacgtcg gaggcatgcg cgtagccgac cgcctcgtcc ttcaccgcgc gcagcccgaa
      481 gccctgtgag gtgtcgtagg tcgcctgctt gagccggcca ttgtcgaaaa gcagcatttc
      541 ggcctggcga tattcgagaa agagctcgcc gtcgtcggcg ccctcgaggc cccgcgagat
      601 gaccttggcg aggcgggtgc ggtcgagccc ggcgcgatcg acgagcgagg aggtcgagtt
      661 catcattgcg ttccttcaat gcggcctttc gcaaagatag gtaatggcgg cccgatccgc
      721 gagcggggtc gccaaatgat gttttcgccc ccgaggccgg catggggtca attgggcgcg
      781 ccaaccggcg gagcttcaga cgctgacgcc cgttcgccgc gaccgacggg ctatcccgac
      841 agccgctcga tgtcggcgcc gcagcgcgcc agcttgtctt ctagccgctc gaaaccgcgg
      901 tcgagatgat agacgcggtt gacgatggtc tcgccctcgg cggcgagcgc ggcaatgacc
      961 agcgacaccg aggcgcgcag atcggtcgcc atcaccggcg cgccggtgag ccgctcgacg
     1021 ccctcgatcg tcgcccgctc gccgtcaagc tggatgcggg cgccgagccg caccagctcc
     1081 tgcacgtgca tgaagcggtt ctcgaagatc gtctcgacga cgtgcgaggt gccgcgcgcc
     1141 cgcgtcatca gcgccatcag ctgcgcctgc aaatcggtcg ggaaggcggg gaacggcgcc
     1201 gtcgccacct cgatcgggac caggtcggcg ccgtcgcgcg cgatgcggat gccctgattg
     1261 gtggatgaca cggtgacgcc ggtttgcgta agtacgtcga gcgcggcctg caacagctcg
     1321 gcgcgcgcgt tctgcaggaa gatgtcgccg ccggccatcg ccgccgccat ggcgtaggtt
     1381 ccggtctcga tacggtcggg caacaccgcg tggcgtgcgc cgccgagctt ggcgacgccc
     1441 tcgacgatga tccgctgcgt gccggcgccg gagatgtgcg cgcccatctt gttgaggcaa
     1501 tcggcgacgt cgacgatctc gggctcgcac gcggcattct cgatcacggt cgtgcccttg
     1561 gccagcgaag ccgccatgac cgccgtatgc gtgccgccga ccgtgacctt gggaaagact
     1621 atcttgccgc ccttgagccc gttcggcgca gccgccacga catagccgct gtcgatctcg
     1681 atgtcggccc cgagctcggc gagcgccatg atcaggaggt cgaccggccg cgtaccgatg
     1741 gcgcagccgc ccggcatcga cacccgcgcc cggcccatgc gcgcaacgag cggcgcgatc
     1801 acccagaagc tggcgcgcat cttggagacg agctcgtagg gcgcggtggt atcgacgata
     1861 ttggccgcgg agacgtgcag ggtctggccg tgatcggcag tctcgccggg ccgcttgccg
     1921 ctgaccatga cgtcgacgcc gtgattgccg aggatgcgct gcaggagcac gacgtcggcg
     1981 agccgcggca cattgtcgag aatgagcgtc tcctcggtga ggaggctcgc gatcatcagc
     2041 gggagcgtgg cgttcttggc tcccgagatc ggtatcgtgc cggtgagacg ccgcccaccg
     2101 acgatgcgaa tacggtccat tgtcacccca gcggccccat cccgggccga ggttgctaat
     2161 cgctgccccg attgcggcga aagtcgggtg actgcgcatg gcgcgccggc gcggcttcgt
     2221 cgccgctcgg gctcgccgcg cggccgcgcg cttgcgcctt gcgccgcttg aggttttcgc
     2281 gcagcgccgc ggagagccgc ttgctgcgcg cttgctgctt taattccttg ccgcgcgaga
     2341 tcatcgccgt tccgcgacct acagcaggtg acccagatcg agcgcgtgcg gtgcgtagac
     2401 gccgagcgcg aggatcgcga gcccgaggat cccaagtgcg ccgctgagcc agacgagcgc
     2461 gatggcgctg gtgatcaccg ccgcggaggc gagcacgatg ccgatctgga acgccgccga
     2521 agcaagctcg tagtgatgat atttcgccat ggcgaggtcg cggccttctt ctgcttcctt
     2581 ggcgcgttcg gaaagctcct tgcgcccttc atgcgtctcc ggctcggaat cgtagcgcgc
     2641 cgcggttttt tgccaagcct cgatctgttt atctattgcg gccttcgccg ccggatcggc
     2701 ggcggccgct gccgtcacct tcaattcttc tgcggcagtc ttaaccagcg tcatgcggac
     2761 ggtcttggcc tgaaagaagg cccagagatt cgaagactcg acattggcgc tgatcgcctc
     2821 ggtctgcgcg ctcttgccca gcgtctccga gaaggagagg aacagcgcca gcaccgcgat
     2881 caacagcgcg atcctgcggt tctcgtgcga agcatgctcg acgtgctccg cctgttccat
     2941 ctgttcgtgt gcttccgcca tgatgtttgc cccggct
//
LOCUS       DASYNS010000449         6255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4638881, whole genome shotgun sequence.
ACCESSION   DASYNS010000449 DASYNS010000000
VERSION     DASYNS010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4638881"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1074)
                     /locus_tag="VGP50_14010"
     CDS             complement(160..1074)
                     /locus_tag="VGP50_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8028544.1"
                     /translation="MRAALALWAIIVTLALPAGAAIAGDAVRVASKIDTEGTLLGNLM
                     LAVLEARGIKATDKLGLGPTNILRKAIIAGEIDLYPEYTGNGAIFYALESDPAWRSAA
                     DGYARINALDAARNLVWLAPAPADNAWAIALRKDVAQANGLASMEDFARWVNADGPVR
                     LAASAEFVESPAALPAFERAYGFTLKRAQILTLAGGNTAVTLRAAAEGTSGVNAAMAY
                     GTDGALAILDLVVMADPKKAQVVYAPAPVIRASVLERHPEIRDGLDPVFRSLDEATLR
                     RLNAAVAVDGEDARHVALEYLRSKGFVR"
     gene            complement(1074..1898)
                     /locus_tag="VGP50_14015"
     CDS             complement(1074..1898)
                     /locus_tag="VGP50_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV8028545.1"
                     /translation="MSAVSEVIPRPASTVLLLRDGPSGLEVLMVTRNVASDFASGALV
                     FPGGRVDAPDGEATTLACCRAGVGADAAAMAFLVAGIRESFEEARVLLARPKGSDRLL
                     SAGALDALEAKLGARLGRAPHFQDLVATGEIELATDLLVPFAHWITPVGPPKRFDTHF
                     FLAPAPADQVAAHDGREAVDTLWIAPQKAVAEADAKRVTLVFATRMNLVKLARSADLA
                     AALAAANGAVIVTVSPELVDSPEGPVLRIPEAAGYGLTEMRADKTVRAWKPPGSGS"
     gene            complement(1954..3264)
                     /locus_tag="VGP50_14020"
     CDS             complement(1954..3264)
                     /locus_tag="VGP50_14020"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEV8028546.1"
                     /translation="MPDALRRLALISGLAGASLGLLLAAEAAEPGGAAMPPVLTGAAA
                     YGDWTTDAPGVRRQITPADLPPPYATDNASNGPRVVTRPRDALPQVPPGFRVEVFASR
                     LDAPRVIRPAPNGDIFIAETGGGAIRILRAGASATEGPGAVYAEGLDEPFGLAFYPPG
                     SDPQYLYVGTVDAVLRFAYRNGDARARGTPERIARLPAGGHSTRDVIFSRDGRKMYVS
                     VGSASNDQEWASDESRRANILELNPDGSGERRFATGLRNPVEMAIHPVTGALWTIVNE
                     RDALGDNLPPDYVTSVRDSAFYGWPWYYIGANQDPRHKGEHAELRDKITVPDVLIQPH
                     SAPLGIAVYTGQQFPVAYRNDLFVALHGSWNRAQRTGYKLVHVALKDGKARGDYEDFM
                     TGFVTGDGNVWGRPVGVAVAPDGALLVSDDGGGVVWRITYTGSP"
     gene            complement(3313..4236)
                     /gene="pqqB"
                     /locus_tag="VGP50_14025"
     CDS             complement(3313..4236)
                     /gene="pqqB"
                     /locus_tag="VGP50_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEV8028547.1"
                     /translation="MRIRVLGAAAGGGFPQWNCNCEVCRRAWSGAVPAQTQASLAVTA
                     DDQTWFLLNASPDLRAQILATPPLQPRRPGRDTPIAGVVLTNGDVDALAGLLSLRESQ
                     PLAVYATGRVLATLAANPIFAVLNPDRVARRALPLGQATDLLTQDGSSSGLRVEAFSV
                     PGKVALYLEDAAIEQRLAERSEDTVGLEITATASGKRLLHIPACAGLDDELRARLRRA
                     PLLFFDGTLWQDDEMIRAGLGAKTGRRMGHLSVSGPDGSMAALAELGIARKLFIHINN
                     TNPILCAGSAERAAVAAAGWEVAQDGLDIRL"
     gene            complement(4264..5190)
                     /locus_tag="VGP50_14030"
     CDS             complement(4264..5190)
                     /locus_tag="VGP50_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV8028548.1"
                     /translation="MIGRGAAILVLWALTPIVAAARAEPVLRVCLDGDLPPDSFHHGA
                     ASGGFDLAVSQALAKHLGQSLAVQWYESKLDLDSSSVLAADALLSDGRCQLVAGYPLV
                     RDALGKPGAPNSRLPDYEGAKPADRRRWVDLGTLVASRPYHQAPLAIVLGANASAHPA
                     ARLMDLQDLKLATESGTLSDAILMNYADGRLVDHLTHLVPGRGELLPRLENGDFDAAL
                     VPLRRFDLYRTEHPNTKLRVAGYYDRVTFNMGFVALSTEAALLDRVDEAIGRMLAESD
                     LPALAEAAGMTYVAPRQPDVLERFSLRDLTGD"
     gene            complement(5187..5585)
                     /locus_tag="VGP50_14035"
     CDS             complement(5187..5585)
                     /locus_tag="VGP50_14035"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV8028549.1"
                     /translation="MQQPSPHFPDASRRVPLSRASGRGARRLHLVLIFSLVAGWPAAA
                     GAADQSAIDPVEQGRTLYALHCSHCHGFNMVNPGTVAFDLRRFPPDDKARFVNSVTQG
                     KNNRMPPWGDVLSPEEIDELWAYIRTGGKS"
     gene            complement(5691..>6255)
                     /locus_tag="VGP50_14040"
     CDS             complement(5691..>6255)
                     /locus_tag="VGP50_14040"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV8028550.1"
                     /translation="QVEVWPSILGGKNWEPVSFDPKRGLIYANTLNWGSNYKAIPADY
                     KAGDWYLGMDLSKGWAWPKNADGSAGPRGYLKAIDPLTGATKWQAESDLPRFSGVLST
                     DGGVVFAGRLTGEFEAFDADTGKKLWSFQTGSGIEGQPVTWQEGKTQYVAVSSGIGGV
                     YSLFSGDERLANVPPGGSLWVFALEGK"
BASE COUNT         1021 a         2124 c         2184 g          926 t
ORIGIN      
        1 gggcaatgcg ccgctgcccc tcaccctccc gttgcttggc aacgggcccc tccctctccc
       61 acggagggga gaggggattt gcgccggctt ttcccctctc cccttgtgcg agagggaggg
      121 gcccggcgct aagcgccggg agggtgaggg gtgcggggct caccgcacga accccttgct
      181 ccgcaaatac tcgagcgcga catggcgggc gtcttctccg tcgacggcga cggcggcgtt
      241 gaggcggcgg agcgtcgctt catcgagcga gcggaagacg gggtcgagac cgtcgcgaat
      301 ctcgggatgg cgctcgagga cgctggcgcg gatgaccggc gcgggcgcgt agacgacctg
      361 ggcctttttt ggatccgcca tgaccacgag gtcgagaatc gcgagcgcgc cgtcggtgcc
      421 ataggccatg gccgcgttga cgcccgaggt gccttcggcg gcagcacgca aagtgacggc
      481 cgtgttgcca cccgcgagcg taaggatctg cgcacgcttc aaggtgaagc cgtaggcgcg
      541 ctcgaaggcg ggaagcgccg ccgggctctc gacgaattcg gccgaggcgg cgagccgcac
      601 cgggccgtcc gcattcaccc agcgggcgaa atcctccatg ctggcgagcc cgttggcttg
      661 cgcgacgtcc ttccgcagcg cgatcgccca ggcgttgtcg gccggcgccg gcgcgagcca
      721 gacgagattc cgcgcggcat cgagcgcatt gatcctggcg tagccgtcgg cggcgctgcg
      781 ccacgcagga tcgctttcca aggcatagaa gatcgcgccg ttgccggtgt attcgggata
      841 aaggtcgatc tctccggcga tgatcgcctt gcgcaggatg ttggtgggac cgagccctaa
      901 cttgtcggtg gccttgatgc cccgcgcctc cagcaccgcc agcatgagat tgccgagaag
      961 cgtgccttcc gtgtcgatct tcgaggcgac gcgcacggcg tctccggcaa tggcggcgcc
     1021 ggccgggagc gccagcgtga cgatgatcgc ccagagggcg agcgccgccc gcatcagctg
     1081 ccggaaccgg gcggcttcca ggcgcgcacc gtcttgtcgg cgcgcatctc ggtcaggccg
     1141 tagcccgccg cctcgggaat gcgcaagacc ggcccctcgg gcgagtcgac gagctcgggg
     1201 ctgacggtca cgatgacggc gccgttcgcc gccgcgaggg ccgcggcaag gtcggcgctc
     1261 ctggcgagct tcaccaggtt catgcgggtg gcgaagacca gcgtcacgcg cttggcatcc
     1321 gcttcggcca cggccttctg cggcgcgatc cagagggtgt cgacggcttc acggccgtca
     1381 tgagcggcga cctggtcggc gggcgcgggc gcgaggaaga aatgcgtgtc gaaacgcttc
     1441 ggcgggccga ccggcgtgat ccaatgggca aagggcacga gcagatcggt cgcgagctcg
     1501 atctcgccgg tcgcgaccag atcctggaaa tgcggtgccc gccccagccg cgcgccgagc
     1561 ttggcctcga gcgcatcgag cgctcccgcg gacagcagac ggtcgctgcc cttcggccgc
     1621 gccagcagca cgcgcgcttc ctcgaagctc tcgcggatac cggcgacgag aaaggccatc
     1681 gcggcggcgt cagcccccac ccccgcgcga cagcaggcca gcgtcgttgc ttcgccatcc
     1741 ggcgcatcga cccggccgcc gggaaacacc agggcgcccg aggcgaagtc cgatgccacg
     1801 ttgcgcgtga ccatcagcac ttcgaggccc gaaggcccgt cgcgcaacag cagaaccgtc
     1861 gacgccgggc gcggtatgac ctccgaaacg gcgctcatgc gacctccctg ccgccgacaa
     1921 taggtcgccc gagtccccgg cgccagccac gcttcaggga gatccggtgt aggtgatgcg
     1981 ccagacgact ccaccgccat catcgctcac caagagcgct ccgtcgggcg ccacggcgac
     2041 gccgaccgga cggccccaga cattgccatc gcccgtgacg aagccggtca tgaagtcctc
     2101 atagtcgccg cgggccttgc cgtccttgag cgcgacatgg acgagcttgt agccggtgcg
     2161 ctgcgcgcgg ttccacgatc cgtggagcgc cacgaagagg tcgttgcggt aggcgaccgg
     2221 gaattgctgt ccggtgtaga cggcgatgcc gaggggcgcc gaatgcggct ggatcagcac
     2281 gtccggcacg gtgatcttgt cgcgcaattc cgcatgctcg cccttgtggc gcgggtcctg
     2341 attggcgccg atgtagtacc agggccaacc atagaaggcg ctgtcgcgga cactcgtcac
     2401 atagtccggc ggcaggttgt cgccgagagc atcgcgctcg ttcacgatgg tccagagcgc
     2461 gccggtcacg ggatggatcg ccatctcgac cgggttgcgc agaccggtgg cgaagcgtcg
     2521 ctcgccgctg ccatcggggt tgagctcgag gatattggcg cgccgcgact cgtcgctcgc
     2581 ccactcctgg tcgttcgacg ccgagcccac cgagacatac atcttgcgcc catcgcggga
     2641 gaagatgacg tcgcgcgtcg agtgaccacc cgcgggaaga cgcgcaatcc gctcgggcgt
     2701 gccgcgggcc ctcgcatcgc cgttgcgata ggcgaagcgg agcacggcgt cgaccgtgcc
     2761 cacatagaga tattgcggat cgctgcccgg cggatagaag gcgagaccga agggctcgtc
     2821 gaggccttcg gcgtagaccg cgcccggacc ctccgtcgcg ctggcgcctg cgcggagaat
     2881 gcggatggcg ccgccgccgg tctcggcgat gaagatgtcg ccgttgggcg ccgggcgtat
     2941 gacgcggggc gcatcgagcc gactggcgaa gacctctacc ctgaagcccg gcgggacctg
     3001 gggcaaagca tcgcgcggcc gcgtcacgac gcgcggccca ttcgaggcat tgtcggtcgc
     3061 ataaggcggc ggcaggtcgg cgggcgtgat ctgccgccgc acgcccggcg cgtcggtcgt
     3121 ccagtcgcca taggcggcag cgccggtgag cacgggcggc atcgcggcgc cgccgggctc
     3181 tgcagcctcc gccgcgagca ggagcccgag cgatgcgccg gcgaggcccg agatcagtgc
     3241 gaggcgacgc aatgcatccg gcatgtcggg ctccctgctc agcatattcc gggcgctaca
     3301 cgaccccgcg attcacaagc ggatatcgag accgtcctgc gccacttccc atcctgccgc
     3361 cgcgacggcc gcgcgctcgg cggagcccgc gcacaggata gggttggtgt tgttgatgtg
     3421 aatgaacagc ttccgcgcga tgccaagctc ggcaagcgcc gccatcgagc catcggggcc
     3481 ggagacgctg agatgcccca tgcgccgccc cgtcttcgca ccgaggccgg cgcggatcat
     3541 ctcgtcgtcc tgccataagg tgccgtcgaa gaacagcagc ggggcccggc gcaagcgcgc
     3601 gcgaagctcg tcatcgagcc cggcgcaagc cggtatgtga aggagccgct tgccgcttgc
     3661 cgtcgccgtg atctcgagcc cgaccgtgtc ttccgagcgc tccgccaagc gctgctcgat
     3721 ggcggcgtcc tcgagataga gcgccacttt tcccggtacg ctgaaggcct cgacgcgaag
     3781 cccgctggaa ctgccgtcct gggtcaggag atccgtcgcc tggcccagtg gcagggcgcg
     3841 gcgggcgacg cgatcgggat tgagcacggc aaagatcggg ttggcagcga gcgtcgccag
     3901 cacgcggcca gtcgcgtaga cggcaagcgg ctggctttcg cgcaggctca acagaccggc
     3961 cagcgcgtcg acgtcgccat tggtcaagac aacgccggcg atcggcgtgt cgcgaccggg
     4021 acggcgcggc tgaagcggcg gcgtcgccag gatctgggcg cgcaggtcgg gcgacgcatt
     4081 gagcaggaac caggtctggt catctgcagt gacggcgagc gatgcctggg tctgggccgg
     4141 cacggccccg ctccaggcgc gccggcagac ctcgcaattg cagttccatt gcgggaagcc
     4201 gccgcccgcc gcggcgccga gcacccggat acgcatcgtc tctcctggcc gcccgctcgt
     4261 cctctagtct ccggtgaggt cgcgcagcga gaagcgctcg aggacatcgg gctgccgcgg
     4321 cgcgacatag gtcatgccgg ccgcctcggc aagggccggg agatcgctct ccgcgagcat
     4381 ccggccgatc gcctcgtcga cacggtcgag cagcgccgcc tcggtcgaga gcgcgacgaa
     4441 gcccatgttg aaggtgacac ggtcatagta gcccgcgacc ctaagcttcg tgttgggatg
     4501 ctcggtgcga taaaggtcga aacggcgcag cggcaccagc gccgcgtcga aatcgccgtt
     4561 ctcgagccgc ggcagcagct cgccgcggcc cggcaccaga tgcgtgaggt gatcgaccag
     4621 gcgcccgtcc gcgtagttca tcaggatcgc atcgctgagc gttccgcttt cggtggcgag
     4681 cttgaggtcc tgcaggtcca tgagccgtgc cgctgggtgg gcagatgcat tggcaccgag
     4741 cacgattgca agtggcgcct ggtgataggg ccggctcgcc accagggtgc cgaggtcgac
     4801 ccagcgccgc cggtccgcag gcttggcgcc ttcgtagtcc ggcaggcgtg aattcggcgc
     4861 accgggcttg cccagcgcat cccgcaccaa cgggtaaccg gccacgagct gacagcgccc
     4921 gtccgagagc agcgcatcgg cagcaagcac cgagctgctg tccaggtcga gcttgctctc
     4981 ataccattgg accgccagcg actgcccaag gtgctttgcc agcgcttgcg acaccgcgag
     5041 atcgaagccg ccgctcgccg cgccgtggtg aaaggaatca ggcggcaagt ccccgtcgag
     5101 acagacacgg agcacgggct cggcacgcgc agcggcaaca atcggcgtca aggcccacag
     5161 aacgaggatc gctgccccgc ggccgatcat gacttcccac ccgtgcgtat gtaggcccaa
     5221 agctcgtcga tctcctccgg cgagagaacg tcgccccaag gcggcatgcg gttgttcttg
     5281 ccctgcgtca ccgagttgac gaagcgcgcc ttgtcgtcgg gcgggaagcg gcgaagatcg
     5341 aaggcgaccg taccggggtt caccatgttg aagccgtggc aatgcgagca atgcagcgca
     5401 taaagcgtcc tgccctgctc gaccggatcg atggcgctct ggtcggctgc gcccgcggcc
     5461 gccggccagc cggcgaccag cgaaaagatg aggacgagat ggagacggcg agcccctcgc
     5521 ccgcttgcgc gagagagagg gacccgacgc gaagcgtcgg gaaagtgagg ggacggttgc
     5581 tgcatggacg ctgagaacgg cccacgcgac cccttacctg cccgcgcctg gggcgtggac
     5641 cccctctccc gcaagcggga gaggggaaga gccgcgtggc tcgccagttc ttacttaccc
     5701 tcgagagcga agacccagag agagccgccg ggcggcacgt tggccagccg ctcgtcgccc
     5761 gagaagagcg agtagacccc gccgatgccg ctggacacgg cgacatactg cgtcttgccc
     5821 tcctgccagg tgacgggctg cccctcgata ccggagccgg tctggaagga ccagagcttc
     5881 ttgcccgtat cggcgtcgaa agcctcgaac tcgcccgtga ggcggccggc gaagaccacg
     5941 ccgccatcgg tcgagaggac gccggagaag cgcggcagat cgctttccgc ctgccatttc
     6001 gtcgctccgg tcagcgggtc gatggctttc agatagccgc gcggcccagc gctgccgtcg
     6061 gcgttcttgg gccaagccca gcccttgctg aggtccattc cgagatacca gtcaccggct
     6121 ttgtagtcgg ccggaatcgc cttgtagttg gagccccagt tcaacgtatt ggcatagatc
     6181 aggccgcgct tgggatcgaa tgagacgggc tcccagttct tgccgcccag gatcgacggc
     6241 catacctcga cctgc
//
LOCUS       DASYNS010000450         8405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5262249, whole genome shotgun sequence.
ACCESSION   DASYNS010000450 DASYNS010000000
VERSION     DASYNS010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5262249"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..509)
                     /locus_tag="VGP50_14045"
     CDS             complement(96..509)
                     /locus_tag="VGP50_14045"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEV8028551.1"
                     /translation="MAAEDNGTSTHASRHPVGETLRRLEGALAARGFIVYCRIDHAAA
                     AATVGEKLLPRTVILYGNPQTGTAAMARRPTLAIDLPAKLLVWQDETGHVWITYNAAR
                     HFLGAVFARHDAEADAAAWHNHDAVMAEVVAHAAE"
     gene            complement(737..1771)
                     /locus_tag="VGP50_14050"
     CDS             complement(737..1771)
                     /locus_tag="VGP50_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8028552.1"
                     /translation="MTRLLPLLLLAFALAACAGEGTGHPSALGAIGNAAPSGPAVQPL
                     LADKAFIADDGVSLPLRVWLPEGEPKAVILALHGFNDYSNAFVAPAAEWAKHGIATYA
                     YDQRGFGEAPLHGRWAGVAQLARDVTAASRLLRARYGATPIYVLGESMGGAVVIVAVS
                     GAAGTPPPDVDGVILSAPAVWGRDMMTVLERVALWTGDAFFPDMTFTGRGLRIMPSDN
                     IQMLRALARDPLVIKETRVDSIKGLVDLMDMALYAAPRVTMPMLMLYGERDEVVPNAA
                     TLRWIETLPYGQRMTRRIALYTAGYHMLLRDLEGPVVMHDVESWVADHQAPLPSGADR
                     NASAMLYARR"
     gene            complement(1768..2787)
                     /locus_tag="VGP50_14055"
     CDS             complement(1768..2787)
                     /locus_tag="VGP50_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028553.1"
                     /translation="MYSAIAPPTALAPEAASAPRLQSAAEPRHAVALEGLTKIYVGDR
                     RTPPKVALDGIDLVIPRGSIFGLLGPNGAGKSTLINILAGLVVKTAGRARIWDIDIDE
                     NPRQARAAIGVVPQELNIDAFFTPRELLELQAGFYGVPRAERRTQEVLAMVGLADQAD
                     SYARTLSGGMRRRLLVAKAMVHSPPVLVLDEPTAGVDVELRQQLWTQIRALNHAGTTV
                     LLTTHYLEEAESLCDRIAIIDKGRVVVCDTTEALVNQVDDKTLRLTLVEAITSLPPAL
                     QRWGLAVTAPGRLLFRYGPSRTELGPLFSAIAAAGLTIRDATTEETDLEDVFLRLTGS
                     NARTS"
     gene            2906..3142
                     /locus_tag="VGP50_14060"
     CDS             2906..3142
                     /locus_tag="VGP50_14060"
                     /inference="COORDINATES: protein motif:HMM:NF021772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEV8028554.1"
                     /translation="MAPFETIVVDHSVVACNGGGGPLGHPRVYLNLGADGKIECPYCS
                     RLFVLRGQTGSGHVAETAPVTPSAHPPPAAPAKP"
     gene            3139..5949
                     /gene="polA"
                     /locus_tag="VGP50_14065"
     CDS             3139..5949
                     /gene="polA"
                     /locus_tag="VGP50_14065"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV8028555.1"
                     /translation="MSSKRRHVYLVDGSGYIFRAYHALPPLTRSDGTPTGAVLGYSNM
                     LAKLLQETDADHLAVVFDRARSSFRTRIYDKYKAQRPEPPSDLVPQFKLVREATDAFN
                     VCAIELDDYEADDLIATYARRAAAEGATVTIVSSDKDLMQLVGDGISMLDPIKQRPIG
                     AAEVREKFGVGPDKVADVQALCGDSVDNVPGVPGIGVKTAAELINTYGDLETLLMHAA
                     EIKQPKRRQALLDFAEQARLSKRLVLLDDQVPLPVPLDALEVKPLSRDKIVPFLRQQE
                     FRALLGRIETRLAKSGNGAAAPAAHASPAPAPAQNGAAAAGDLAKIERRYALVQDVAD
                     LDAWIAAAQARGAVALDTESTLLNAARAELVGIALALAPGEACYIPLAHRTGAAEPGL
                     DLDAAAPRQIPLDLAIARLKPLLEDDGVLKIGHDIKDDVEVMARYGIALAPIDDAMLI
                     SYVLDGGLHGHGIEELAETHFGHATIAYKDVAGSGKSHVGFAAVPLEKARDYAAEKVD
                     LALRLHRLLKPRLLAEHRLAFYETIERPLVPVVARMERAGIRIDRAELNRLSQDFAGR
                     LAELEREIFRLAGHPFNIGSPKQLGEVLFDELGFDGGKKNKTGAYGTDAALLEQLALT
                     HDLPARVLDWRQLAKLKSTYADALVDEIEPETGRVHTSFALAATLTGRLSSIEPNLQN
                     IPIRTEEGRKIRRAFVAEPGHLLISADYSQIELRLAAHIADVGPLKEAFRSGNDIHAL
                     TASEVFGVPMAQMTGEIRRRAKAINFGIMYGMSAFGLAQRIQVPQAEAAEYIKAYFLR
                     FPGIRDYMERVKAECRDKGYVETLLGRRCYIPGIKDGNWSRRGYAEREAINAPLQGTA
                     ADIIKRAMARVPPALAQAGLKARMLLQVHDELVFETPDAEAEEAAAVIKRVMEQAPLP
                     ALVLSVPLVVETGAAGNWDEAH"
     gene            complement(7156..7908)
                     /locus_tag="VGP50_14070"
     CDS             complement(7156..7908)
                     /locus_tag="VGP50_14070"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8028556.1"
                     /translation="MTTERTDRIKAAADEGRADAQWAMGLFLEQGFQAPADLAQAAVW
                     YERAANQGLAVAQFDLAHLYWRGAGVAQSDAVGLEWCRKAAEGDFALAQYTLGIWYLE
                     RMGDAQKAMHWLQRAADGRNSAAAFRLGSIYEQGRGVKVNPDEALAWYARGEALGDPQ
                     ATYHLARLYEDRVKGPTDNTTVLELYEKAAARGAAAACLALAKAYDQGLLGLEKDAIK
                     AAEYVAKFTIMRDASKRHLREALRELKEDRTG"
     gene            complement(7905..>8405)
                     /locus_tag="VGP50_14075"
     CDS             complement(7905..>8405)
                     /locus_tag="VGP50_14075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEV8028557.1"
                     /translation="WDAALRPFGRIEQESFTFTQRLRFPGQYHDIEDKLFYNVFRDYD
                     PDLGRYIESDPIGLAGGTNVYAYVRGNPVNRTDPFGLFMWDNSCPDMCKTALEDLQFM
                     LVEINLIRRSMELTNPARLMLLMFYVDLYNEVWYWYDKNCPPPLYPKMPGPLTPFGLR
                     KNESPP"
BASE COUNT         1449 a         2818 c         2720 g         1418 t
ORIGIN      
        1 ctcgcccggg ccggcgccga cgagccacac cgagcccggc gcgaaatccg gcagatcgag
       61 cggaagagaa aacggtgcca tgccggatcg gagtctcact cggcggcgtg cgcgaccact
      121 tcagccatga ccgcgtcatg gttgtgccag gccgccgcat cggcctccgc gtcatggcgc
      181 gcgaacaccg cgccgaggaa atggcgcgcg gcgttgtagg tgatccacac gtgtcccgtc
      241 tcgtcctgcc acaccagcag cttcgccggc aggtcgatgg cgagcgtcgg ccggcgcgcc
      301 atggcggccg tgccggtctg cgggttgcca tagagaatca cggtgcgcgg cagaagcttc
      361 tcgccgacgg tcgcggccgc cgcggcatgg tcgatgcggc aatagacgat gaagccgcgc
      421 gcggcgagcg ccccttcgag ccggcgcagc gtctcgccca cgggatggcg gctcgcgtgg
      481 gtgctggtcc cattgtcctc tgccgccatg cgcgatccct tctccactcc gggattggag
      541 cgcgcgggcg cgccggcgtc aacctcatcc cctctcccct tgtgggagag ggaggggccc
      601 gacgcgaagc gtcgggagcg tgaggggtac cccggctcga tcggagatcg tcctgcccgg
      661 tgcggctctc gcgattgctg acgcaatcgc tgtcgccgcc ggcggtaacg cgctctgcgc
      721 gttaccgccg cgcgcgttac cgccgtgcgt agagcatcgc gctcgcgttg cggtcggcac
      781 ccgagggaag cggggcctgg tggtcggcga cccagctctc gacgtcgtgc atcaccaccg
      841 gtccttcgag gtcgcgcagc agcatgtgat agccggccgt atagagagcg atgcgccgcg
      901 tcatccgctg cccgtaaggg agcgtctcga tccagcgcag agttgcggca ttcggcacga
      961 cctcgtcgcg ctcaccataa agcatcagca tcggcatggt gacgcggggc gccgcgtaaa
     1021 gcgccatatc catgagatcg accaggccct tgatcgaatc gacgcgcgtc tccttgatta
     1081 ccagcggatc gcgcgccagc gcgcgcagca tctggatatt gtccgacggc atgatgcgca
     1141 agccgcggcc ggtgaaggtc atgtccggaa agaaggcgtc gcccgtccac agcgcgacgc
     1201 gctcgagcac cgtcatcatg tcgcgtcccc agaccgcggg ggccgagagg atgacgccgt
     1261 cgacgtcggg gggcggcgtg ccggcggcgc cgctcaccgc gacgatgacg acggcgccgc
     1321 ccatgctttc gccgaggaca tagatcggcg tcgccccata gcgcgcccgc agcagccggc
     1381 tcgccgccgt gacgtcgcgc gcgagctggg cgacgccggc ccagcgccca tgcagcggcg
     1441 cctcgccgaa gccgcgctgg tcgtaagcat aagtggcgat gccatgcttg gcccattccg
     1501 ccgccggcgc gacgaaggcg ttcgaatagt cgttgaagcc gtgaagcgcc aggatcaccg
     1561 ctttgggctc gccctcgggc agccagacgc ggagcggcag gcttacgccg tcatcggcga
     1621 tgaaggcttt gtcggcgagg agcggctgga cggcgggacc cgacggcgcg gcattgccga
     1681 tggcgccgag cgcgctggga tggccggtgc cctcgccggc acaggcggcg agcgcgaatg
     1741 cgagcagcaa caagggcaag agacgggtca tgacgtcctc gcattggagc cggtgagccg
     1801 caggaataca tcctcgagat cggtctcctc ggtggtcgcg tcgcggatcg tcagaccggc
     1861 ggcggcgatc gccgagaaaa gcgggccgag ctcggtgcgg ctcggaccat agcggaagag
     1921 gagacggccg ggcgcggtca cggcgaggcc ccagcgctgc agcgccggcg gcagcgacgt
     1981 gatcgcctcg accagggtca ggcgcagcgt cttgtcgtcg acctggttca ccagcgcctc
     2041 ggtggtatcg cagacgacga cacgcccctt gtcgatgatg gcgatgcggt cgcagagcga
     2101 ttccgcctct tcgagataat gcgtggtgag gagcacggtc gtgccggcgt ggttgagcgc
     2161 gcggatctgc gtccagagct gctggcgcag ctcgacgtcg acgccggccg tcggctcgtc
     2221 cagcacgaga acgggcggcg aatgcaccat cgccttcgcc accaggagtc gccggcgcat
     2281 gccgcccgag agcgtgcggg cgtaagaatc ggcctgatcg gcgaggccga ccatcgccag
     2341 gacctcttgc gtgcgccgct cggcgcgcgg cacgccgtag aagccggcct gcagctccag
     2401 gagctcgcgc ggcgtgaaga aggcgtcgat attgagctcc tgcggcacga cgccgatggc
     2461 ggcgcgcgcc tggcgcggat tctcgtcgat gtcgatatcc cagatgcggg cgcggcccgc
     2521 ggtcttcacc accagcccgg ccaggatgtt gatgagcgtc gatttcccgg cgccgttggg
     2581 gccgagaagg ccgaagatcg agccgcgcgg gatgacgagg tcgatgccgt cgagggcgac
     2641 cttgggcggc gtgcgccggt cgccgacata gatcttggtg aggccttcga gcgccaccgc
     2701 gtgacggggc tcggccgcgc tctgcaggcg cggcgctgaa gcggcctcgg gggccagtgc
     2761 ggtgggagga gcgatcgcgg agtacattga ttgtgccggc cccatgcgta tcatccgcgc
     2821 ggcggcccgg tcaacccgcg cccgccgatt ccgcagggga atgatgcttc cgttaacgtt
     2881 aaccgcggct taagccgccg gagtcatggc acctttcgag acgatcgtcg tcgatcacag
     2941 cgtggtcgcc tgcaacggcg gcggcgggcc gctcggccat ccgcgcgtct atctcaacct
     3001 gggcgctgat ggcaagatcg agtgccccta ctgctcgcgc ctgttcgtgc tgcgcggcca
     3061 gacgggctcg ggccatgtcg ccgagacggc gcccgtcacg ccctccgcgc atccgccgcc
     3121 ggccgctccc gccaagcctt gagcagcaag cggcggcacg tctatctcgt cgacggctcg
     3181 ggctacatct tccgcgccta tcatgcgctg ccgccgctga cgcgcagcga cgggacgccc
     3241 accggcgccg tgctcggcta ttccaacatg ctggcgaagc tgctgcagga gacggacgcc
     3301 gaccatctcg cggtggtctt cgaccgcgcc aggtcgagct tccgcacccg catctacgac
     3361 aaatacaagg cgcagcggcc ggagccgccg tccgatctcg tgccgcaatt caagctggtg
     3421 cgcgaggcga ccgatgcgtt caacgtctgc gcgatcgagc tcgatgatta cgaagccgac
     3481 gatttgatcg cgacgtatgc gcgccgcgcc gccgccgagg gcgcgaccgt gaccatcgtc
     3541 tcctccgaca aggacttgat gcagctcgtg ggcgacggca tctcgatgct cgacccgatc
     3601 aagcagcgtc cgatcggcgc ggccgaagtg cgcgagaaat tcggcgtcgg cccggacaag
     3661 gtggccgatg ttcaggcgct ttgcggcgac agcgtcgaca acgtgccggg cgtgccgggc
     3721 atcggcgtca agaccgccgc cgagctcatc aatacctatg gcgatctcga gaccctgctg
     3781 atgcatgcgg ccgagatcaa gcagccgaag cggcgccagg ccttgctcga tttcgccgag
     3841 caggcgcggc tctcgaagcg gctcgtgctg ctcgacgacc aggtgccgct gccggtgccg
     3901 ctggacgcgc tcgaggtgaa accgctctcg cgcgacaaga tcgtcccgtt cctgcgccag
     3961 caggagttcc gggcgctgct gggacgcatc gagacgcggc tcgccaagag cggcaatggc
     4021 gcggccgcgc cagcggcgca cgcttcgccg gcgcccgcgc ccgcgcaaaa cggcgccgcc
     4081 gctgccgggg atctcgccaa gatcgagcgc cgctacgcgc tggtgcagga cgtggccgac
     4141 ctcgacgcct ggatcgcggc cgcccaagca cgcggcgcgg tcgcgctcga tacggaaagc
     4201 actttgctca atgcggcgcg cgccgagctc gtcggcattg ccctcgcgct cgcgcccggc
     4261 gaggcctgct acattccgct cgcgcatcgt accggcgccg ccgagcccgg cctcgatctc
     4321 gacgctgcag cgccccggca gatcccgctc gacctcgcga tcgcccgctt gaagccgctc
     4381 ctcgaagacg acggcgtgct caagatcggc cacgacatca aggacgacgt cgaagtgatg
     4441 gcgcgctacg gcatcgcgct ggcgccgatc gacgacgcca tgctgatctc ctatgtgctc
     4501 gacggtggct tgcacggcca tggcatcgag gagctcgccg agacgcattt cggccacgcc
     4561 accatcgcct acaaggacgt cgccggctcc ggcaagagcc atgtcggctt cgccgcggtg
     4621 ccgctcgaga aagcgcgcga ctacgcggcc gagaaagtcg atctggcgct ccgcctccat
     4681 cgcctgctca agccgcgcct cctggccgag caccgccttg ccttctacga gacgatcgag
     4741 cggccgctcg tgccggtcgt cgcccggatg gagcgcgccg gcatccgcat cgaccgcgcc
     4801 gagctcaacc gcctgtcgca ggattttgcc ggccgcctcg ccgagctcga gcgcgagatc
     4861 ttcaggctcg ccggccatcc cttcaacatc ggctcgccca agcaacttgg cgaggtgctc
     4921 ttcgacgagc tcggcttcga cggcggcaag aagaacaaga ccggcgctta tggcacggac
     4981 gcggcgctcc tcgagcagct tgcgctcacc catgacttgc cggcccgcgt cctcgactgg
     5041 cgccagctcg cgaagctcaa aagcacctat gccgatgcgc tggtcgacga gatcgagccc
     5101 gagacggggc gcgtccatac gtccttcgcc ctggcggcga cgctgacggg gcggctctcc
     5161 tcgatcgagc ccaatctgca gaacatcccg atccgcaccg aggaaggccg caagatccgc
     5221 cgcgctttcg tcgctgagcc cggccatctc ctgatctcgg ccgattacag ccagatcgag
     5281 ctccggctgg ccgcgcatat cgccgatgtc gggccgctca aggaggcctt ccgctccggc
     5341 aacgacattc acgcgctgac ggcgagcgag gtcttcggcg tgcccatggc gcagatgacc
     5401 ggcgagatcc gccgccgcgc caaggccatc aatttcggca tcatgtatgg catgagcgct
     5461 ttcggcctgg cgcagcgcat ccaggtgccg caagccgagg cggccgaata catcaaggca
     5521 tatttcctgc gcttccccgg catccgcgac tacatggagc gcgtcaaggc cgagtgccgc
     5581 gacaaaggct atgtcgagac gctgctgggc cggcgctgct acatccccgg catcaaggac
     5641 ggcaactggt cgcggcgcgg ctacgccgag cgcgaagcga tcaacgcccc gctccagggc
     5701 accgccgccg acatcatcaa acgcgccatg gcccgcgttc cccccgctct cgcccaggcc
     5761 ggcctcaaag cgcgcatgct cctccaggtg cacgacgagc tcgtcttcga gacgccggat
     5821 gccgaagccg aagaagccgc cgccgtcatc aagcgcgtga tggagcaagc gccgttaccg
     5881 gcgttggtgc tgtcagtgcc gctcgtcgtc gagactggcg ccgcggggaa ctgggacgag
     5941 gctcactgat cgacgcgaag cgtcgtctca ggcgggcgac agcggttgct cttgcaaccg
     6001 cgaaagcccg caccccggca gaacaagatc caccaccgag gcagtgaggc cttcaccgat
     6061 gcggaaaatc aactatcgcg gtagggaccg ctcattgctg agcgcccccc gcacagatgc
     6121 gtgtacgtgc ccgattgaga cggcgaagcg tttgcttggc cagggatgaa ggatgcgagg
     6181 cttgggcagc caccgcgctg gtcagatggc gtcggcgctc cgatcgatgc tttcgcggct
     6241 cattggctgg cgtacacgtt ccgctgccga cgcttcgcga ccggtcgacg cacggctcgg
     6301 ggccgatgtg gttcgccacg ccttcatcgt agtgggcttg caccacctac tccttgccgg
     6361 tcgcccggcg cacctgaccc caattcttcc ccaattcttc cgcggtgctc tcggccggat
     6421 cgttcgggaa gccgcggcgt tctggctcgc acctgcacgg gacgtcaagg cgggcggtcc
     6481 gggatagcgc ccagggttct ggactggtgc cactcgcgtg ttatgcttgt ccttcgcgtt
     6541 cccgcgggtt acggcctacg atgcaggtcc atgacatgat cggctatttc gacggtccaa
     6601 ggtttcgcgc caagctgatg cgccatattg aaaacgggaa atcattggtt aggatcgaac
     6661 ggatagaagg aatagaggtc gttcacgaag atatggagat tccgaccgac cagatcccta
     6721 tcgatttacg gcaaattggc tctattttcc tcgttttgat caactgcgcc ctctcgagcc
     6781 agaggtcaac cgaaggcgct tccacgatat acgtccgtgt tagccgactt ccccaatcct
     6841 caaagtgacc cctaatctag gcgggcaagt cggtccgacg gtccgcacat tagccaaaga
     6901 gctacgctag agcgtttctc gaaaagattg aatcgggcga ttcccaaagg cgctgaaatg
     6961 agattcaagc tccctgctgg aactggaggc cagcgtggga tggggaaacc gtattcggaa
     7021 gacttgcgtg gtgccgcggt tcgagcaatc gaggccggcc acacccgtga agaggtcgcg
     7081 gagctttatg gcataagcct gagctcggtg ggccggttcg taaagcgctg gcgctcgacc
     7141 ggcagggaaa ccgctctagc cggtgcgatc ctcctttaat tcacgtagcg cctcccgaag
     7201 atggcgctta ctggcatcac gcatgattgt aaacttggcg acgtattccg ctgcctttat
     7261 cgcgtctttt tccaagccga gcaatccctg gtcgtaagcc tttgcgagag caagacacgc
     7321 cgctgccgca ccgcgagcgg ccgccttttc gtagagttcg agcaccgttg tattgtccgt
     7381 aggtccttta acacggtcct catatagcct agccagatga tacgtcgcct gaggatcccc
     7441 aagagcttct ccacgcgcat accatgcgag cgcctcatcg ggattgactt tcacacctct
     7501 gccctgctca tagattgatc ccagtcgaaa cgcggcagcc gaattgcgac catccgccgc
     7561 gcgctgcaac cagtgcatgg ccttttgtgc atctcccatc cgttcaagat accagatccc
     7621 gagcgtatac tgggcgaggg cgaaatcacc ctctgcggcc ttcctgcacc attccaatcc
     7681 gacggcatcg gattgcgcga caccggcccc tcgccagtag agatgcgcaa gatcaaattg
     7741 agcaacggca agtccttggt tcgcagcgcg ctcgtaccat accgccgcct gcgcaaggtc
     7801 ggccggcgct tgaaagcctt gctctaagaa caatcccata gcccattgcg cgtcggcgcg
     7861 tccttcgtcc gccgcagctt tgattcgatc tgttcgctca gtggtcatgg tggtgattcg
     7921 ttcttccgca acccgaaagg cgtaagcggg cccggcattt tcgggtaaag aggtggcgga
     7981 caattcttat cataccaata ccaaacttca ttgtacagat ccacgtagaa cattaatagc
     8041 attaatctcg cgggatttgt tagctccatg ctacgcctta tgaggttgat ttcgaccaac
     8101 atgaactgca agtcctccaa ggctgttttg cacatgtccg gacaagaatt gtcccacata
     8161 aagaggccga acgggtctgt gcgattgacc gggtttcccc ggacatacgc atagacgttt
     8221 gtccctcctg cgagcccgat cggatcgctc tcgatgtatc gtcccagatc gggatcgtaa
     8281 tcgcgaaaca cgttgtaaaa gagcttgtcc tcgatgtcgt ggtactggcc gggaaagcgc
     8341 aggcgctggg tgaaggtgaa gctctcctgc tcgatccgcc cgaagggccg cagcgccgcg
     8401 tccca
//
LOCUS       DASYNS010000451         4607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_115791, whole genome shotgun sequence.
ACCESSION   DASYNS010000451 DASYNS010000000
VERSION     DASYNS010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115791"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..2044)
                     /locus_tag="VGP50_14080"
     CDS             complement(227..2044)
                     /locus_tag="VGP50_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028558.1"
                     /translation="MTMAQNGSLFGFFDKNPLPMWIEDATTARFLAVNDSAVKFYGYT
                     REHLLGMTAATLAVPADAQAPAGVARHHTADGTIIELRLETSSGEIDGRSARLVVVID
                     ESAQIKAERALYESERLLEAASDYSWEQDAQNRVTRLSANYEALFGLPPSEMLGKRFT
                     ESRDLRITPEMGRMVLLAMKARQPYRDFVYSRKAPDGKIRWFKLSGTPILDKGGVFQG
                     YRGVGAEITSHVEAEQAARLAHRSLHDAVAHVTQPFVVYDAANRAIAFNQAFTDLFKQ
                     AVSKSPVVQGVSFRELAEWQLGFGFYAEGAQPPVTVEMLLEHYRSEREHTYHLRDGRW
                     MLVVYRRLPGDGRVGLWTDVTAIKRAETERHVLEAQLHHSQRLEALGALAGGVAHEIN
                     NALVPVVALTKIVAAKLPEGSRERRNLDTVLGAAERSRDLVKQILAFSRKEQEQQRRG
                     SVDVAAVMREALHLLRVTVPSSIRIEEDIAPAPPVTGDPSQLHQVIVNLATNAAQAID
                     EAHGTITLGLRAEADGAALRLWVADTGAGMDEATKARIFEPFFTTKEVGRGTGLGLAV
                     VHGIVKDHGGRIEVESAPGRGTRFDIVLPARAAEAGAAA"
     gene            complement(2055..4433)
                     /locus_tag="VGP50_14085"
     CDS             complement(2055..4433)
                     /locus_tag="VGP50_14085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8028559.1"
                     /translation="MTAKRRKRTASPNARPKRDLEARLAEKDRALAECERRNHALCES
                     EQRYKHLFEVASDWFWEADAAGRLSYVSSNIETVLGLPVSAYLGKRLAETEGVSIDAD
                     AGRATLDALKARRSYRDFVYSRKLANGQVVWINSSGAPRYGADGAFLGYSGVARDVTA
                     QVEAEHRLQRLERQYRQLFEDSTDSYWEQDVQGRFTHLSSSFETSTGIPVVELLGRRL
                     KEVSLVKVDPKSDEQTLATIKARVGYRNLVQTLVRPDGRLVYVGTSGIPVFGENGEFR
                     GYCGISKDITAQVEAERALRESEQRFRELFEIASDYFWEADEHQRITYLSENYEAVFG
                     IPPSQTLGKRLTETPDVSVDPEMGKMAIAAIRARQPYRDFVYSRKFPDGKTRWFKTSG
                     IPIVDKDGTFRGYRGVGADMTAHVEADQAARLAQSRLHDAVAHVTQPFVFYDAQHRAT
                     AFNQAFTDLHREPGGTAPVSQGVSFRELAEWQLRVGFYAEGPDEDEITVDALVEEYES
                     ARESTHHLRDGRWMLVVYRRLPGDGRVGLWTDVTEIKRAESERRRLEAQLLHSQRLEA
                     LGTLAGGVAHEINNALVPVIALTKLVAGRLPEDSRDRRNLATVMIGAERSRDLVKQIL
                     AFSRKEEEGHRRESVDIGAVLRDALQLMRATVPTSIRLEEEIAAVPPVAGDPNQLHQV
                     IVNLVTNAAHAIGEAHGTITVGLRTDADGKALRLSVADTGSGMDAATKARIFEPFFTT
                     KEVGEGTGLGLAVVHGIIKDHGGRIEVESTPGHGTCFDVVLPVTAAVAGAAA"
     gene            complement(4451..>4607)
                     /locus_tag="VGP50_14090"
     CDS             complement(4451..>4607)
                     /locus_tag="VGP50_14090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028560.1"
                     /translation="EVGKGTGLGLAVVHGIIKEHGGRIEVQSTPGKGTRFDVVLPAQT
                     AQTGAAA"
BASE COUNT          737 a         1543 c         1506 g          821 t
ORIGIN      
        1 cgacgccgac acgagtgccg cagcacggcg acgtcagcgg gcgccttcga ccgaagcgct
       61 cgcctttgac gagtcggccg ctgccggaag atagacgtcg aagcgcgagc ccgtgccgac
      121 ttcgcgctcg cgacggcgat acggccgcca tgctgggcca cgatgccgtg agcgaccgcg
      181 aggccgagcc ccgtgccgac gccaacctcc ttcatggtca gggatttcag gccgccgcgc
      241 cagcctcggc ggcccttgcg ggcaggacga tgtcgaagcg ggtgccgcgg ccgggcgcgc
      301 tctcgacctc gatgcggccg ccatggtcct tgacgatgcc atgcaccacc gcgaggccga
      361 gtccggtgcc cctgcccacc tccttcgtcg tgaagaacgg ctcgaagatc cgcgccttcg
      421 tcgcctcgtc catgccggcg cccgtgtccg ccacccagag gcgcagcgcg gcgccgtcgg
      481 cttcggcacg cagccccagc gtgatcgtgc catgcgcctc gtcgattgcc tgcgccgcgt
      541 tggtcgcgag gttcacgatc acctgatgga gctggctcgg gtcgccggtc accggcggcg
      601 ccggcgcgat gtcctcctcg atacgaatgc tgctcggcac ggtcacgcgc agcaagtgca
      661 gcgcctcgcg catcaccgcg gcgacatcga cgctgccgcg ccgttgctgt tcctgttcct
      721 tgcggctgaa ggcgagaatc tgcttgacga ggtcgcgcga gcgctcggcc gcgccgagga
      781 cggtatcgag gttgcgccgc tcgcggctcc cctccgggag cttggcggcg acgatcttgg
      841 tgagggcgac cacggggacg agggcgttgt tgatttcatg cgcgacgccg ccggcgagcg
      901 cgcccagcgc ttcgagccgt tgcgagtggt ggagctgcgc ctcgagcaca tgccgctcgg
      961 tttcagcgcg cttgatggca gtgacgtctg tccataagcc gacccggccg tcgccgggca
     1021 agcgacgata gacgaccaac atccagcggc catcccgcag atgataggta tgctcgcgct
     1081 cggagcggta atgctcgagc aacatctcga cagtgacggg cggctgggcg ccctcggcgt
     1141 agaaaccgaa gcccagctgc cactcggcaa gctcgcggaa ggagacgcct tgaacgacgg
     1201 gcgacttact gaccgcctgc ttgaagaggt cggtgaaggc ctggttgaaa gcgatggcgc
     1261 gattcgccgc gtcatagacg acgaagggct gggtcacatg ggcaaccgca tcgtgcaggc
     1321 tccggtgggc caaacgcgcc gcctgctctg cctccacatg ggacgtgatc tcggcaccga
     1381 caccgcgata gccctggaac actccgccct tgtccaggat cggtgttccg ctcagcttga
     1441 accatcggat cttgccgtca ggggctttgc gcgaatagac gaaatcgcgg tagggttgtc
     1501 tcgccttcat ggccaagagg accatcctgc ccatctctgg cgtgattcgg agatcgcggc
     1561 tctcggtaaa gcgcttgccg agcatctcgg agggcgggag gccaaatagg gcttcgtagt
     1621 tggcggaaag gcgagtcacg cggttttgcg catcctgctc ccaagagtag tcggatgccg
     1681 cctcgagcaa ccgctcgctt tcgtacaaag cgcgctcggc cttgatttgc gcgctctcat
     1741 cgatgacgac gacgagacgg gccgagcggc cgtcgatttc gccggagctg gtttcgagcc
     1801 gtagctcgat gatcgtgccg tcggccgtgt ggtggcgcgc gacgcccgcc ggcgcctgtg
     1861 catcagccgg caccgccagg gtggcggcgg tcatgccaag aaggtgctca cgggtgtagc
     1921 cgtaaaactt gaccgcgctg tcgttcacgg ccaggaaacg cgcggttgtt gcatcctcga
     1981 tccacatcgg cagcgggttc ttgtcgaaga agccgaagag cgagccgttc tgcgccatcg
     2041 tcatcaccgg agcctcaagc cgctgcgccg gcgacggccg ccgttacggg cagcacgacg
     2101 tcgaagcagg tgccgtggcc gggggtactc tccacctcga tgcggccgcc atggtcctta
     2161 atgattccgt gcaccaccgc gagaccgaga ccggtgccct cgccgacttc ctttgtcgtg
     2221 aagaacggct cgaagatgcg ggccttggtc gcggcgtcca tgccgctacc ggtgtcggcg
     2281 accgagaggc gcaatgcttt gccatccgcg tcggtgcgca gtcccaccgt gatcgtgcca
     2341 tgcgcctcgc cgatcgcgtg tgccgcgttg gtgacgaggt tcacgatcac ctgatggagt
     2401 tggttcggat cgccggccac cggcggtacc gcggcgatct cctcctcgag acggatgctt
     2461 gtcggcaccg tcgcgcgcat cagctgcaac gcatcgcgca acacggcgcc gatgtcgacg
     2521 ctctcccggc ggtgtccttc ctcctccttc cggctgaagg cgaggatctg cttcaccagg
     2581 tcgcgcgaac gttcggcgcc gatcatcacg gtggcgagat tgcggcggtc gcggctatct
     2641 tccggcaggc ggcccgccac cagcttggtg agggcgatca ccgggacgag cgcgttgttg
     2701 atctcatgcg cgacgccgcc ggcgagcgtg ccgagtgcct ccagccgctg cgagtgcagc
     2761 agttgcgcct cgagtcgccg gcgctcggac tcggcgcgct tgatctccgt cacatccgtc
     2821 cagaggccga cgcgtccgtc gccgggcagg cggcgataga ccacgagcat ccagcgacca
     2881 tcgcggagat gatgggtgct ctcgcgcgcg ctctcgtatt cctcgacgag cgcatcgacc
     2941 gtgatctcgt cctcgtccgg accctcggca tagaagccga cgcgcaactg ccattcggcg
     3001 agctcgcgga acgagacgcc ctggctgacc ggcgccgtgc cgccgggctc gcgatggaga
     3061 tccgtgaagg cctgattgaa cgcggtggcg cgatgctggg cgtcatagaa gacgaagggc
     3121 tgcgtcacat gcgcgacagc gtcatgcaag cggctttgcg ccaatcgcgc tgcctgatcg
     3181 gcctcgacat gcgccgtcat gtcggcgccg acgccgcgat atccgcggaa cgtcccgtcc
     3241 ttgtcgacga tcgggatgcc gctcgtcttg aaccaccgcg tcttgccatc cgggaacttc
     3301 cgggaataga cgaaatcgcg atagggttgc cgcgccctga tcgcggcgat cgccatcttt
     3361 cccatctcag gatcgacgga cacgtcgggc gtctcggtga gccgtttgcc cagggtctgc
     3421 gagggcggaa tgccgaacac cgcctcataa ttctccgaga gataggtgat gcgctgatgc
     3481 tcatcggcct cccagaagta atccgaggcg atctcgaaca actcgcggaa ccgctgctcg
     3541 ctctcgcgga gcgcccgctc ggcctcgacc tgtgcggtga tatccttgga tatgccgcaa
     3601 tagccgcgga attcgccgtt ctcgccgaac accggaatgc cgctggttcc gacatacacg
     3661 aggcgaccat cggggcggac aagtgtttgg acgagattgc ggtaccccac ccgcgccttg
     3721 atggtggcga gcgtctgctc atccgatttc ggatcgacct tgaccaggga aacctctttg
     3781 aggcgccttc ccagcaattc cacgacgggg atgccggtcg acgtctcgaa gctggacgag
     3841 aggtgcgtga agcgcccttg cacatcctgt tcccaatatg agtcggtcga gtcctcgaag
     3901 agctgacggt attgccgttc gagccgctgc agccgatgct cggcttcgac ttgggccgtc
     3961 acgtctcgtg cgacgccgct gtagcccagg aaggcgccgt ccgcgccata gcgtggcgcg
     4021 ccgctcgaat tgatccagac gacctggccg ttcgccagct tgcgcgaata gacgaagtcg
     4081 cggtaggacc gccgcgcctt gagcgcgtca agggtggctc taccagcatc ggcgtcgatg
     4141 ctcacgccct cggtctcggc cagacgcttg ccgagatagg ccgataccgg aaggccgagc
     4201 acagtttcga tattggacga gacatagctg agccgacccg cggcgtccgc ctcccagaac
     4261 cagtccgatg ccacctcgaa gaggtgctta taccgttgct cgctttcgca gagcgcgtga
     4321 ttgcgacgct cgcattcggc aagcgcccgg tccttctcgg cgagtcgcgc ctccagatcc
     4381 cgcttgggcc gggcattcgg gctcgccgtt cgcttgcgcc gcttagcggt caattctctc
     4441 tccacattcc tcaagctgcg gcgccggtct gcgcggtctg cgccgggagc acgacatcga
     4501 aacgcgtgcc tttgccgggt gtgctctgca cctcgatgcg gccgccatgc tccttgatga
     4561 tcccatgcac cacggcaagg ccgaggccgg tgcccttgcc gacctcc
//
LOCUS       DASYNS010000452         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_115921, whole genome shotgun sequence.
ACCESSION   DASYNS010000452 DASYNS010000000
VERSION     DASYNS010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115921"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VGP50_14095"
     CDS             complement(<1..641)
                     /locus_tag="VGP50_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028561.1"
                     /translation="MQIPESSNEDPNDIGFRAHANVRVLVPAGGMNDVAPPAHAALTK
                     GLAVEPDAAAPYSGYWFLETPASLACVYKLVTQVAGCNPYSATANASGGSRAIAIVDA
                     YDNPSATTDLAHFSTQFGLPAANFQVVYASGRKPSYDSGWQIESSLDIEWSHAMAPSA
                     RIYLVEAASNSFSDLFTAVTAAAKLVAAAGGGEVSMSWGGSEFSGESAYDGYFA"
     gene            complement(1278..>2080)
                     /locus_tag="VGP50_14100"
     CDS             complement(1278..>2080)
                     /locus_tag="VGP50_14100"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HEV8028562.1"
                     /translation="RGLVSGTLTVEVLTEGVHSGDASGIVPSSFRILRQLLSRLEDET
                     TGAIRVAALHADIPADRRAQAERAAAALGDEIYTKFPLVPGLAPVATDPTELVLNRTW
                     RPQLAVTGMAGLPAPGNAGNVLLPFTAAKLTLRLPPTLDGPRAVELVGKLLEADPPYG
                     ARIRFDGKESSTGWNAPALAPWLGRSLADAAEAAFHAPPAYMGEGGTIPFMAMLGEKF
                     PHAQFVVTGVLGPQSNAHGPNEFLHIPTAKRLSAVIARVLADHHAKAA"
BASE COUNT          343 a          659 c          759 g          319 t
ORIGIN      
        1 gcgaaatatc cgtcataggc gctctcgccc gagaactcgc tgccgcccca gctcatcgac
       61 acttcgccgc cgccggccgc cgcgacgagc ttggccgccg ccgtcaccgc ggtgaagaga
      121 tcgctgaagc tgttggaggc cgcctcgacc aggtagatcc tggcgctggg cgccatggca
      181 tgggaccatt cgatatcgag cgaggactcg atctgccagc cgctgtcgta ggacggcttt
      241 ctcccgctcg catagacgac ctggaaattc gcggccggca agccgaactg cgtcgagaaa
      301 tgcgcgagat cggtggtcgc cgaggggttg tcataggcat cgacgatggc gatggcgcgt
      361 gagccgccgc tggcattcgc cgtcgcgctg tagggattgc agcccgcgac ctgggtcacg
      421 agcttgtaga cgcaggcgag cgaggccggc gtctcgagga accaatatcc ggaataaggc
      481 gccgccgcgt cgggctcgac ggcgaggcct ttcgtcagcg ccgcatgggc cggtggtgct
      541 acgtcgttca tgccgcccgc gggcaccagc acgcgcacat tggcgtgggc gcggaagccg
      601 atgtcgttgg gatcctcgtt gctcgactca ggaatctgca cctgtccgcc gcgcggcggc
      661 ggagagccag ccggcgctcc cgcgctcgac aaagaggcgg ccgacgttcc cgcgctcgac
      721 agagaggcgg ctgatgccca aggcgtggtc gcgagaagcg cagcgaagga gagagcgagg
      781 gtcggagtcg ataagccgga cgtgctgctc ttgaaacgga tcggcgtcgc catttagcca
      841 cctcaatgtc tgatgtggcc cccgaagcga tgacttgtcg acgatacagg cgcggcgtta
      901 aggggcaatt atgagagggc ttagctgctc ggccgatgct ggcgaggaca gtcgaggcgc
      961 aggcggacta cgtccaaggc gcggatgcgt ggacattcgg agggacgcgc gttcttgcgc
     1021 acgcgacgcg ctacggcatt cacagatctg gcaatgccgt tccctctccg cccctcaggg
     1081 gcggagaggg tcagggtgag gtggggcgcg tggcgcccga ccgctgcaga acgcatccga
     1141 tatcgacgct ccgatcggga atcttgttcc cgaccaaggc atcagcgttc cgaatttgcg
     1201 gcgccccacc tcaccccagc cctctccgcc cctcgggggc ggagagggag tcacgcgcga
     1261 tgtgtgaatc gacgagctta cgcggccttc gcgtggtggt cggcgaggac gcgggcgatg
     1321 acggcggaga ggcgctttgc ggtcgggatg tggaggaatt cgttcgggcc gtgcgcgttc
     1381 gattgtggtc cgagcacgcc cgtgacgacg aactgcgcgt gcgggaattt ctcgcccagc
     1441 atggccatga acgggatcgt gccgccctcg cccatatagg cgggcggcgc atgaaaggcg
     1501 gcttccgccg cgtcggcgag ggagcggccg agccacggcg ccaaggccgg cgcgttccag
     1561 ccggtggacg attccttgcc gtcgaagcgg attcgcgcgc cataaggcgg atcggcctcg
     1621 agcagcttgc ccacgagctc gaccgcgcgc gggccatcga gagtcggcgg caggcgcagg
     1681 gtgagcttgg ccgcggtgaa gggaaggagc acgtttccgg cattgcccgg cgccggcaat
     1741 cccgccatgc ccgtgacggc aagctgcggc cgccaggtgc ggttgagcac gagctcggtc
     1801 ggatcggtgg cgaccggcgc aagaccgggg acgagcggga acttggtgta gatctcatcg
     1861 ccgagcgcgg ccgccgcccg ctccgcctgg gcacggcgat cggccgggat gtcggcgtga
     1921 agcgccgcga cgcggattgc gcccgtcgtc tcgtcctcga gccgcgacag gagctggcgc
     1981 aggatgcgga agctcgacgg cacgatgccc gatgcatcgc cggagtggac gccctccgtc
     2041 agcacctcga cggtgagcgt gcccgagacg aggccgcgca
//
LOCUS       DASYNS010000453         3855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4327972, whole genome shotgun sequence.
ACCESSION   DASYNS010000453 DASYNS010000000
VERSION     DASYNS010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4327972"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /gene="hemA"
                     /locus_tag="VGP50_14105"
     CDS             <1..1306
                     /gene="hemA"
                     /locus_tag="VGP50_14105"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEV8028563.1"
                     /translation="AVTSERAAMPGTEAAGTGIGLLVIGTNHRSSPLELRDRFALVEA
                     DLPVALEKLRDAAIGEAALIATCDRIELVTTAADADRASAVLVAILADRMGLPAATIA
                     ASLYRHAGAAALRHLFAVASALDSLVIGEPQVLGQVKAAHRLAAEAGLAGAELESAFA
                     AAYAAARRVRRDTRIAERPVSIAAAALQLARDIHGDFDRCAALLLGTGEMGELMADQF
                     HRAGIARLVVCGPPVSAARTAQRFACNFVAVEELDDALAAADIVIAALGSGRAVLTVP
                     RVAAALRKRPKRPIFVIDAAIPADAEPAINDLNGAFLYDLGDLERTALAGRATREAAS
                     AEAWRIIDAELAAFAERRAERRAVPAIVALRGHVEALRQQVLAESGGDAEAATRLLVN
                     RLLHDPSEVLRELGGAAPSEIDAIEELMRRLFRLGGAQEKSE"
     gene            1330..2385
                     /gene="prfA"
                     /locus_tag="VGP50_14110"
     CDS             1330..2385
                     /gene="prfA"
                     /locus_tag="VGP50_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV8028564.1"
                     /translation="MLDKVTRRHAELRDALGAPGLPGSEVAKLAKEYSDLTPLVDSIA
                     ALRKAEAEMAEVAALAEDGSDPEMRAVVQNEMQALREKLPQLEQQVKVLLLPKDEADE
                     RNAILEVRAGTGGEEAALFAADLFRMYQRYAAQHGWRFEVMEVSETGLGGFKQAIASI
                     SGRDVFHRLKFESGVHRVQRVPETEGSGRKHTSAATVAVLPEAEEVDIHIEEKDLRID
                     VFRSSGPGGQSVNTTDSAVRITHLPTGVVVSQQDEKSQHKNKAKAMKVLRARLYERER
                     QAKDAVRAASRKSQVGSGDRSERIRTYNFPQGRVSDHRINLTLHKIDKVMSGEALDEF
                     VEALLAEDQAARLADME"
     gene            2382..3284
                     /gene="prmC"
                     /locus_tag="VGP50_14115"
     CDS             2382..3284
                     /gene="prmC"
                     /locus_tag="VGP50_14115"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV8028565.1"
                     /translation="MSGEATLCSKDLATVGAALRWGAARLARAGIAEPRREARLLLAL
                     ATGVEPGVILGYPERELDAAAQQLLVPLVERRAAREPFSRIAGRRQFWSLDLEISADT
                     LDPRPDSETLVEMALASLPDRRAALRILDLGTGSGALLLALLKEMPRAIGLGVDLLPG
                     AAATARRNAATLGLGRRALFLGGSWGQAISSGWADVILANPPYITTSEIDRLAPEVAR
                     YEPRMALDGGPDGLGAYRELVNEIPRLLAPGGIVVLEVGAGRADPVTELLVKAGLVLR
                     GVRRDLAGIERCIAAASPEGRPSR"
     gene            3467..>3855
                     /locus_tag="VGP50_14120"
     CDS             3467..>3855
                     /locus_tag="VGP50_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEV8028566.1"
                     /translation="MMSYRDFKGVMRPGQNKRNRGRNNNNNNNGNHPNNNRPRPPHRV
                     QTFDSNGPNVKIRGNAYQVFERYIALAREAVSSGDRVAAENFYQHAEHYFRLMNADGH
                     GQGNVAAPRPMTPADTEMTGPESESEG"
BASE COUNT          635 a         1296 c         1358 g          566 t
ORIGIN      
        1 ggcggtcact agcgagagag cggccatgcc cggcaccgaa gcagccggga cgggtatcgg
       61 attgctcgtc atcggcacca accatcgctc aagcccgctc gagctgcgcg accgcttcgc
      121 gctggtcgaa gccgacttgc ccgtggcgct cgaaaagctg cgcgacgcgg cgatcggcga
      181 ggcggcgctc attgccacgt gcgatcgcat cgagctcgtc accaccgcgg ccgacgcgga
      241 ccgcgccagc gccgtgctcg tcgcgatcct cgccgaccgc atgggccttc ccgcggcgac
      301 catcgcagcg tcgctctatc gccatgccgg cgcggcggcg ctgcgccatc tctttgccgt
      361 cgccagcgcg ctcgacagcc tggtgatcgg cgagccgcag gtgctcgggc aagtgaaggc
      421 ggcgcatcgc ctcgcggccg aggcggggct cgccggcgcc gagctcgaat ccgcgtttgc
      481 ggccgcctat gccgcggcgc gccgcgtgcg ccgcgacacg cgcatcgccg agcgcccggt
      541 atcgatcgcc gcggcggcgc tgcagctcgc gcgcgacatc catggcgatt tcgaccgctg
      601 cgcggcgctg ctgctgggca cgggcgagat gggcgagctg atggcggatc agttccaccg
      661 cgcgggcatc gcccgcctcg tggtgtgcgg cccgccggtg agcgccgcgc ggacggcgca
      721 gcgcttcgcc tgcaacttcg tcgcggtcga ggagctggac gacgcgctgg cggcggccga
      781 catcgtcatc gccgcgctgg gaagcggccg ggccgtgctg accgtgccgc gcgtcgccgc
      841 cgccttgcgc aagcggccga agcggcccat cttcgtcatc gacgcggcga ttccggccga
      901 tgcggagccc gcgatcaacg atctcaacgg cgccttcctc tatgacctgg gcgacctcga
      961 gcgcaccgcc ctcgctgggc gggcgacccg cgaggcggca tcggcggagg cctggcggat
     1021 catcgacgcc gagctcgccg cctttgccga gcggcgcgcc gagcgccgcg ccgttccggc
     1081 gatcgtggcg ctgcgcggcc atgtggaagc gttgcgccag caagtgctgg ccgagtccgg
     1141 cggcgacgcc gaagcggcga cgcggctcct cgtcaaccgg ctgttgcacg atccctcgga
     1201 ggtgctgcgc gagctgggcg gggcggcgcc gagcgagatc gacgcgatcg aagagttgat
     1261 gcgccgcctg ttccgattgg gcggcgcgca ggagaagagc gagtgagcgc ggcaagcccg
     1321 ctatacgcaa tgctggacaa ggtcacgcgc cgccatgccg agctgcgcga cgctttgggc
     1381 gcgccggggc tgccgggttc cgaggtggcg aagctcgcga aggaatacag cgatctcacg
     1441 ccgctggtcg acagcatcgc cgctctgcgc aaggccgagg ccgagatggc cgaagtcgcg
     1501 gctctggccg aggatggcag cgatcccgag atgcgcgcag tggtgcaaaa cgagatgcaa
     1561 gccttgcgcg agaagctgcc gcagctcgag caacaggtga aagtgctctt gctgcccaag
     1621 gacgaggccg atgagcgcaa cgccatcctc gaggtgcgcg ccggcaccgg cggcgaggag
     1681 gccgcgctct ttgccgccga tctcttccgc atgtatcagc gctatgccgc gcagcatggc
     1741 tggcgcttcg aagtgatgga agtgtcggaa acgggattgg gtggcttcaa gcaggcgatc
     1801 gcctccatca gcgggcgcga cgtgttccat cgcctcaaat tcgaatcggg ggtccaccgc
     1861 gtgcagcggg tgcccgagac cgaaggcagc ggccgcaagc acacctcggc ggcgacggtc
     1921 gccgtgctgc cggaggcgga ggaggtggac atccatatcg aggagaagga tctgcgcatc
     1981 gacgtcttcc gctcgagcgg cccgggcggg cagtcggtca acaccaccga cagcgccgtg
     2041 cgcatcaccc atctccccac cggcgtcgtc gtctcgcagc aggacgagaa gtcgcagcac
     2101 aagaacaaag ccaaggcgat gaaggtgctg cgcgcgcgcc tctatgagcg cgagcgccag
     2161 gcgaaggacg cagtacgcgc cgcctcgcgc aagagccagg tcggcagcgg cgaccgctcc
     2221 gagcgcatcc gtacttataa tttcccgcaa ggacgcgtca gcgaccatcg catcaacctg
     2281 acgctccaca agatcgacaa ggtgatgagc ggcgaagcgc tcgacgagtt cgtcgaggcg
     2341 ctcctggccg aggaccaggc cgcgcgtctc gcggacatgg aatgagcggc gaagcgaccc
     2401 tttgctccaa agacctggcg acggtgggcg ccgcgctgcg ctggggcgcg gcccggctgg
     2461 cgcgtgccgg catcgcagag ccgcggcgcg aggcgcggct gcttcttgcg ctcgcgaccg
     2521 gcgtcgagcc cggtgtcatc ctgggctatc ccgagcgcga gctggatgcc gcggcgcaac
     2581 agcttctcgt gccgctcgtc gagcggcgcg cggcgcgcga gcccttctcg cgcattgccg
     2641 gacgccgcca gttctggagc ctcgatctcg agatctcggc cgataccctc gatccccggc
     2701 cggacagcga gacgttggtc gagatggcgc tcgcctcgct tcccgaccgc cgcgcggcgc
     2761 tccgcatcct cgatctcggc accggcagcg gcgcgctgct gctggcgctg ctcaaggaaa
     2821 tgccgcgagc catcggcctc ggtgtcgatc ttctgccggg ggcggcggcg accgcgcggc
     2881 ggaatgccgc aacgctcggc ctgggccgcc gcgcactctt tctcggcggc tcctgggggc
     2941 aggcaatttc ttcgggctgg gccgatgtta ttctcgccaa ccctccctat ataaccacga
     3001 gcgagatcga tcgattggcg cctgaggttg cgcggtacga gccgcgcatg gcgctcgacg
     3061 gcggtcccga cggactgggg gcctaccggg agctggtgaa cgagattcct cggctgttgg
     3121 cgccgggcgg catcgtggtg ctggaagtcg gcgcaggccg ggccgatccc gttacggaat
     3181 tgttagtcaa ggcagggcta gtgttgaggg gcgtaaggcg tgatctcgcc ggcatcgagc
     3241 gctgcatcgc ggcggcgtcc ccagaaggcc gaccaagcag gtgaggtatc cgaaaaaaat
     3301 tgttggaata cgcggtgttc ccgtctaggg tggagccgcg aagagatgga gcccaaaggc
     3361 tcctgcgccc agagggcgcc ggtatcgctc tcctaccgta aatggtgctg accgcgatgc
     3421 ggacggacgc cgagggagca cccgcaccaa tcacaaccat cgtgtgatga tgtcgtatcg
     3481 cgatttcaaa ggtgtcatga gaccgggcca gaacaagcgg aatcgaggcc gtaataataa
     3541 caataacaat aacggcaatc atcccaacaa caatcgtccg cgcccgccgc accgcgtgca
     3601 gacgttcgac agcaacggcc cgaacgtgaa gatccggggc aatgcctatc aggtattcga
     3661 gcggtatata gcgctggcgc gcgaggcagt ctcctccggc gatcgcgtcg ccgcggagaa
     3721 cttctatcag cacgccgagc actatttccg cctcatgaat gccgacggcc atggccaggg
     3781 caatgtggcc gcgccgcgtc cgatgacgcc agcggacacg gagatgaccg gccccgagtc
     3841 cgagtccgag ggcga
//
LOCUS       DASYNS010000454        19866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1206848, whole genome shotgun sequence.
ACCESSION   DASYNS010000454 DASYNS010000000
VERSION     DASYNS010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 19866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19866
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1206848"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VGP50_14125"
     CDS             complement(<1..368)
                     /locus_tag="VGP50_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEV8028567.1"
                     /translation="MEPNAAAPLREMWYFALWGAALRRGRMLAKTILGEPLLLCRAAD
                     GEVFALRDICPHRGMPLSCGAFDGGEVECCYHGWRFDRNGQCTAIPSLVPEQEFDIAR
                     IRVKRYAAVEVQGNIWVYLG"
     gene            618..1067
                     /locus_tag="VGP50_14130"
     CDS             618..1067
                     /locus_tag="VGP50_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV8028568.1"
                     /translation="MDDSRNSEPDAAGYSLEDLSVGMSATYERAVTEADVVKFAQISG
                     DHNPVHLDEAFALQTRFKGRIVHGMLSASFLSTVIASRLPGPGTIYLAQNLSFRAPVR
                     IGDKVEAKVTVIDIVPDRARVMLKTVCRVGETVVIDGDALVMVPVRG"
     gene            1148..2095
                     /locus_tag="VGP50_14135"
     CDS             1148..2095
                     /locus_tag="VGP50_14135"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV8028569.1"
                     /translation="MRIFRHDTVAAAERGAVVAIGNFDGVHLGHQAVIGETGRIARAQ
                     NAPLAVLTFEPHPRRVFRPDDPPFRLTPFRAKAQAIEALGVDLLVSLHFDHAFAQLSA
                     EDFIDQVLVRHLGVRHVAIGFDFTFGHGRRGTATLLQAEGRARGFAVTVVEPVTAAGG
                     AVYSATRIREHLVAGRPREAAALLGRFWEIEGRVERGDQRGRGIGFPTANLALDDYLR
                     PAAGVYAVRAAVETEGGVLWHDAVANLGRRPTFGGSDLRLEVHLFDFDADLYGRHLRV
                     ALIEHLRPEQKFAGIDALKAQIAADAAQARAILKTTRAF"
     gene            2172..3365
                     /locus_tag="VGP50_14140"
     CDS             2172..3365
                     /locus_tag="VGP50_14140"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV8028570.1"
                     /translation="MAYVRGRVIHDADSHIMELPGYLDGYVEARFKPQLNADASFARR
                     PKLGSQVPEILRRHDDPVWRADDDAQIMLRKNWDATGAFRREDRPKALDHLGFASQLV
                     FTTALLSHINKLEHGDDIDLAYAAAAAHNRHVVDFCAADRRLLAVGYVPLMDFARTQE
                     AARQAIALGCKALMIPSQCPKAHAPSHIGLDPLWAHAQEAALPIIFHVGGGGPLVSPA
                     NFVNGLPPVPDFHGGDDNFRSIDYMAISYPVMQALATLILDRVLDRFPRLKIGVIEQG
                     ASWLPGWMRSMDSAYLAFYKNEERLKAMSLKPSEFVRRQVRVTPYPHEDTGWTIANSG
                     EEICLFSSDYPHVEGGRNPLKRFEESLVNTPASAKQRFYCDNFIDLMGEGLPADLRHP
                     VPSAA"
     gene            3552..6398
                     /gene="ileS"
                     /locus_tag="VGP50_14145"
     CDS             3552..6398
                     /gene="ileS"
                     /locus_tag="VGP50_14145"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV8028571.1"
                     /translation="MATDYRSTVFLPHTDFPMKGDLPKREPQLLERWSRIDLWNRQRA
                     LFKGREKFVLHDGPPYANGNIHLGTALNKILKDVVSRAQNMLGKDSVYVPGWDCHGLP
                     IEWKIEEQYRAKKKSKDEVPIVQFRKECRDFAAHWVDVQRAEFQRLGVIGDWEHPYLT
                     MSFAAEAQIAREIGKFLMNGGLYRGSKPVMWSVVEQTALAEAEIEYYDHTSPTIWVRF
                     PVAQSPRAELAGASVVIWTTTPWTIPGNRAIAYGADFDYVLVEVTAAAEGSLARPGER
                     FLVVEPLVVATAEVAGITGHIRLAKLKGADLAGTICRHPLHGQGYDIAVPLLPGGFVT
                     TEQGTGFVHIAPGHGADDYELGSRHGIEVPQTVAADGSYYPHVPLFAGKQVYRPNGKP
                     GDANPAVIAALRDAGALLAEGTLVHSYPCSWRSKAPLIFRNTPQWFISMEKNQLRQKA
                     LAAIGETRWIPPQGENRIRSMIETRPDWVLSRQRAWGVPIVVFVDKKTGEPLRDQSVV
                     DRIADAFERDGSDAWYAADPRVFLGPGRDPADYEQVKDIVDVWFDSGSTHAFVLEKRS
                     DLKWPASLYLEGSDQHRGWFHSSLLEACGTRGRAPYEAVLTHGFFVDEQGRKMSKSLG
                     NTVAPQEIVAKSGADILRLWVVSSDYSEDLRIGPEILKYQADAYRRLRNTLRYLLGSL
                     AGFTEAERVSLDAMPELERWVLHRLHELDQVIRRSTEAYDFHAIITALHNFCAVDLSA
                     FYFDVRKDSLYCDPPAAPRRRTARTVLDRVFDSLARWLAPMLCFTAEEAWICRHGDAA
                     ETSVHLVTYAELPASWGNDALGGKWAEIRELRRVVTGALELERAQKRIGSSLQAAVTV
                     YAQPRHVALLEGLDLAEICITSSATLTTAPAPADTFTLPDIAGIAVSVAAAPGEKCQR
                     CWRILPEVGSLSAHPDLCERCADAVDRLGVAVAD"
     gene            6398..6877
                     /gene="lspA"
                     /locus_tag="VGP50_14150"
     CDS             6398..6877
                     /gene="lspA"
                     /locus_tag="VGP50_14150"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV8028572.1"
                     /translation="MLKRGLVVAALVALADQLSKAWVLSFFAEPGSGLHLHPVTGFFN
                     LVLTWNRGVSFGLFNNDDAMNALVFTGAAAIIVAGLVLWLHRTREPLLGVAIGLVIGG
                     AIGNVIDRITRGAVVDFLDFHLAGWHFFAFNLADSAITVGVFFMVIDSLFARREAPN"
     gene            6852..7499
                     /locus_tag="VGP50_14155"
     CDS             6852..7499
                     /locus_tag="VGP50_14155"
                     /inference="COORDINATES: protein motif:HMM:NF022679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HEV8028573.1"
                     /translation="MPDAKRLIRRLESAMSTRPIILSLCALALASPSLSGCTDMKRAL
                     GMEKVVPDEFAVVQGAPLAMPPDFSLRPPKPGAPPSQEVSPTEQARQTIFRAGDQQAA
                     AVPDADQLSPGENSLLRQAGAANAPRDIRETVTRDAMQTPPPPDESFIDKLLFWRTPG
                     KTANAADSVLDPAAEAQRLKAGGAAATGTGTPAAPVPELIGTPTIERRRDPSTTS"
     gene            7542..8885
                     /locus_tag="VGP50_14160"
     CDS             7542..8885
                     /locus_tag="VGP50_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV8028574.1"
                     /translation="MARLAPWGLVLALLLACGTAHADTGKQFNAETFTLANGLQVVVV
                     ENHRAPIATQMIWYKVGSADEPRGKTGIAHFLEHLMFRGTKETPPGAFSRIVAQNGGR
                     ENAFTTADYTGFFQNVAADRLDLVMRLEAERMADLVINDTVVLPEREVILEERRTRID
                     NNPTALLNEQVNAALFLHHPYRNPIIGWEHEMHELGTADALTFYRTWYAPNNAVLVVA
                     GDVTVAGVRALAEKFYGPIPSRALPERHRVGEPPKVAAASLVMKSARVGEPTWSRSYL
                     APSYAAGAKQYAYALQVLAEVVGGGSTARLYRALVLDKGLALNAGSFYNPNALDYGVF
                     GWYATPQKGVAIADLEAAVEGEIKKVLTDGVTAEEVERAKRRMQPQAVYARDSLDGPA
                     RVIGEGLATGRSLDEIETWPQHIGAVTLDDVNAAARSVIHDDTAVTAVLLPEPTS"
     gene            9017..10318
                     /locus_tag="VGP50_14165"
     CDS             9017..10318
                     /locus_tag="VGP50_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV8028575.1"
                     /translation="MLALPARAAEKIQEVTSTGGITAWLVEDHSLPVVTLDVTFRGGA
                     ALDPADKAGRATLTTDLLDEGAGDLDSQAYQGRMEDLAASLQFTAAPDGISASLRSIT
                     ANLAPALELLRLSLTAPRFDEAAVARVRSQLVAELARDVREPRYIADRLWWRNAYDGH
                     PYAQPLKGTAESIARITIADLQALVHDRFARDVMLIGVVGDVTPDALKTLLDKTFGAL
                     PPHAAPGMVAEVTAAPKKALLLAKLPIPQSVVVFGEAGIKRDDPDWYPAFVVNHILGG
                     GGFTARLTAEVREKRGLAYSVYSTLAPLRHSGLILGGVATQNARVAQSIEIIRAEWRR
                     MRDEGPTAAELDDAKTYLTGSYPLSLDSTGSIAATLVAMQRDQLGIDYLERRNALIGA
                     VTLDDAKRVAHRIFDPDKLSFVVIGSPEGLTGAEEVAPSGS"
     gene            10389..11696
                     /locus_tag="VGP50_14170"
     CDS             10389..11696
                     /locus_tag="VGP50_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV8028576.1"
                     /translation="MLYRQDVTGCLAETVGARGLTASELDAVLARARPALKRLKERRG
                     GGSLPLLQLPSRRDDLAPLAAIADEFRRRFDHVVVLGIGGSSLGGQALHALADQGFGP
                     RPGAPRIHFMENIDPATFAALFAALDPARTGVIAISKSGSTAEPSAQLYLCLDWLRQA
                     LGTGDLADRVLVITEPRDNPLRRLAMAQNMRILDHDTNIGGRYSVLSNVGVLPALIAG
                     VDATALREGATMALDATLSAAEPREAPAALGAAVSVGLARRRGIGITVLMPYADRLAA
                     FGLWFRQLWAESLGKDGKGTTPIRALGAVDQHSQLQLYLAGPADKMFTLVMFDAAGNG
                     GLVPPDLASDRDVAYLSGRRLGDLLDAEQRATAATLIARGRPTRVFALARLDEASLGA
                     LLMHFMLETMIAAELFEVNAFDQPAVEEGKVLARHYLGQMKRS"
     gene            11840..13654
                     /gene="mutL"
                     /locus_tag="VGP50_14175"
     CDS             11840..13654
                     /gene="mutL"
                     /locus_tag="VGP50_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV8028577.1"
                     /translation="MTIRQLPPVLVNRIAAGEVVERPASAVKELVENAIDAGATRVEV
                     VLRDGGQSLIAVTDDGGGMTRAELALAVERHCTSKLPEDNLLSIRSLGFRGEALPSIG
                     AVSRLTITSRPPDAAEAWSLAVEGGVKAAAQPAAHPAGTRVEVRDLFYATPARLKFMK
                     TARSERDQALDTVERLAMAYPAIAFMVIGDEDRVLLRLNALGADLAGHDPQTARRTRL
                     AAILGRDFAENALAIDATRENIRLTGLAGLPTLNRATARDQYLFVNGRPVRDKLLVGA
                     VRGAYQDFLARDRHPMVALFVDCPEQEVDVNVHPAKAEVRFRDTALVRGLIVGALRQA
                     LAAAGHRASTTVAEAALAAFRPGAAPSGSGWRHESGTAIPAGLAEAAAAYHAPLPGLA
                     IDRPMARAAEAVVDDTASGLYPLGAACAQLHETYIVAQTNDGIVIVDQHAAHERLVYE
                     RMKAALAGTGVARQILLLPEVVELDEGAASRLAARQAELAELGLVLELFGTGAVVVRE
                     MPALIGEADIQGLVRDLADELAELGDALALKERLEEVCGTLACHGSVRAGRRLSRAEM
                     DALLRQMEATPHSGQCNHGRPTYVELSLIDIEKLFGRR"
     gene            13954..14130
                     /locus_tag="VGP50_14180"
     CDS             13954..14130
                     /locus_tag="VGP50_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028578.1"
                     /translation="MPRIAIVVARTDALCARLNVGLMAVALVLTILTGALSVVRAAQW
                     VESHDPEWLVATDQ"
     gene            complement(14134..14697)
                     /gene="rsmD"
                     /locus_tag="VGP50_14185"
     CDS             complement(14134..14697)
                     /gene="rsmD"
                     /locus_tag="VGP50_14185"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV8028579.1"
                     /translation="MRIIAGRHKGRRLTAPPGEAVRPTSDRARKGLFDILAHGRFAEG
                     PVFEAVPVLDVFAGTGAFGLEALSRGASHATFIEKDRTALAALRSNITALGEAERSTV
                     LAGDARRPPRAAAPAAVAFLDPPYREELATPALASLAACGWLAPEALVVVEVSARQAL
                     ELPPGFTVLEERRYGAARFVFLRRDGS"
     gene            complement(14672..15430)
                     /locus_tag="VGP50_14190"
     CDS             complement(14672..15430)
                     /locus_tag="VGP50_14190"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEV8028580.1"
                     /translation="MPEKGERIAKRLARAGLCSRRDAERWIAQGRVAVGGKVLDSPAL
                     TVMPGDDIRVDGKPLPSDEPARLWRYHKPPGLVTTHHDPQGRPTVFDRLRGELPRVIS
                     VGRLDLNSEGLLLLTNDGGLARALELPSRGWLRRYRLRVHGAVDPARLAALEAGITIG
                     GVAYGSIRAMLDRQQSSNAWLTVSLREGKNREIRRVMEHLGLAVTRLIRIAYGPFQLG
                     ELPRGAVEEVPRRVLREQLGDETSIRHAHHRRPA"
     gene            complement(15432..16148)
                     /locus_tag="VGP50_14195"
     CDS             complement(15432..16148)
                     /locus_tag="VGP50_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV8028581.1"
                     /translation="MAQPVIGLTLDSEPPGGYSKTHPWYALRENYATAVARAGGLPIL
                     LPHEPEQTDAYLALIDGLVVTGGAFDVDPALFGATTRHASVTTKDRRTTFELAITRGA
                     VKADKAVFGICGGQQLLNVALGGTLIQHIPDEVEGALAHEQPNPRTEPGHVVRIAEGT
                     LLRRITGLAELPVNSAHHQAVKDLAPGFVVDAVAPDGVIEGIEDPRRRFLIGVQWHPE
                     YAISEGDTRLFAAFIAAALR"
     gene            16224..16646
                     /locus_tag="VGP50_14200"
     CDS             16224..16646
                     /locus_tag="VGP50_14200"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV8028582.1"
                     /translation="MSHPRIVALALPVRTDDIDFAGVVGNIVYVRWLEDLRLAMLADA
                     YPIARALSDGAAPILLETQIAYERPVTIQDAVSGRMSVTEMGRVKWHVAAEFAVGERV
                     HAHAAQVGIFIRLATRRPIAPPLPLRERFRPVSGPLQG"
     gene            16643..17101
                     /locus_tag="VGP50_14205"
     CDS             16643..17101
                     /locus_tag="VGP50_14205"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV8028583.1"
                     /translation="MSTERTSFMALALAEAERAGERGEVPVGAVLVDGVTGAVLAAAG
                     NRVEALADPTSHAEMLVLRAGAAARGAARLDDCDLYVTLEPCAMCAAAIAFARIRRLY
                     FGAYDPKGGAVEHGPRFFDQPTCHHRPEVYGGIEERRAAELLRKFFKERR"
     gene            complement(17111..17950)
                     /locus_tag="VGP50_14210"
     CDS             complement(17111..17950)
                     /locus_tag="VGP50_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV8028584.1"
                     /translation="MKKARTPHPPGPRKEARDLLRIAGLPAVSALFATAPERVERLFF
                     DERAKPSLGTFCATLARARKPYRLVGAEELERVAGTLLHGGVVALAQPRPVAMLDAAE
                     AARWARDGDLLLLLDGIGNPHNLGAIARSAAFFGLSRLVMSDHPEQAGPSDAAYRVAE
                     GGLEHLALYRAPRFAQTLKDLGRLYRVLGTAAREGTPLDPLERSDRPIALVLGNEEDG
                     LPRATLAACDAIVTIPGSGSVQSLNVSASAAILIHALTQRNIPSPLVGEGGARAKRGR
                     VRG"
     gene            complement(17947..19224)
                     /gene="purD"
                     /locus_tag="VGP50_14215"
     CDS             complement(17947..19224)
                     /gene="purD"
                     /locus_tag="VGP50_14215"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV8028585.1"
                     /translation="MRVLVIGSGGREHALCWAIVASPLCDALFSAPGNPGIAEEGATC
                     VPVAASDVDGILALCRRERIDFVVVGPEVPLVAGLVDRLEAAGVAAFGPSAAASVLES
                     SKGFTKDFCVRHGIPTAAYGRFGDAASAAEFVRKQGVPIVVKADGLAAGKGVTVAMTV
                     AEALAAVNDALVARRFGAAGAEVVVEEFLEGEEASFFALVDGRHALPLATAQDHKRVG
                     DGDTGPNTGGMGAYSPAPCVTATLEREIMARFINPTVKGMAAEGRPFKGLLYAGLMLT
                     KAGPRLIEYNVRFGDPECEALMPRLMSDLLPALIAARDGVLDGFDLRWHSESSVCVVL
                     AAQGYPGEPLRDTEIRGLADATRGDPTVKIFHAGTKRVNGRLLADGGRVLDVVALGKD
                     VAAARAKAYGAIERIDWPEGFYRRDIGWRALPS"
     gene            complement(19245..19760)
                     /locus_tag="VGP50_14220"
     CDS             complement(19245..19760)
                     /locus_tag="VGP50_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8028586.1"
                     /translation="MNAAEMRVRRLASTEAALYRDIRLEALQQSPEAFSSTFESEHPK
                     PLTWFADRLTGSDIFGAFVDGALVGTAALLVAQGLKENHKGFLWGMYVEPQARRIGIG
                     RQLCQTVVAVAASRLERILTAVVAGNEQAHRLYTSVGFEEYGIARNALKQNGRYYDEI
                     LMAKVLVPSSN"
BASE COUNT         3250 a         6851 c         6499 g         3266 t
ORIGIN      
        1 tcgccgagat agacccagat gttgccctgg acctcgaccg ccgcgtagcg cttcacgcga
       61 atccgcgcga tgtcgaactc ctgctccggc accagcgaag ggatcgcagt gcactggccg
      121 ttccggtcga agcgccagcc atgatagcag cactcgacct ctcctccgtc gaaagcgccg
      181 caactcaagg gcatgccgcg atgaggacag atgtcgcgga gcgcgaagac ctcgccatcg
      241 gcggcgcggc acagcaacag cggctcgccg aggatcgtct tcgccagcat tcgcccgcgc
      301 ctgagcgcgg cgccccagag ggcgaaatac cacatctcgc gcaagggggc ggcggcgttg
      361 ggttccatca ggggcgcgag agtagcggcg gccggtcgaa atggccatcc gacgccggcg
      421 tatgtcccga gggacagtcc cggcttgcga ttgtctcgca accgcgagac aaaatcaccg
      481 tctaaacgtt agtctaggag ctaacggcga gcattggctt gccgccactt ctcgttgccg
      541 ttaatttgca ctgcaccata agagagccga attcatcgac cgggcgtcgc aagaacttta
      601 atccgttgga gtgactgatg gacgattctc gcaacagcga acccgatgcg gccggctaca
      661 gcctcgaaga cctgtcggtc ggcatgagcg caacatacga gcgcgcggtc accgaggcgg
      721 atgtggtgaa attcgctcag atctcgggcg atcacaaccc ggtgcatctc gacgaggcct
      781 ttgcgctcca gacccgcttc aagggccgca tcgtccacgg catgctctcg gcgagctttc
      841 tctcgaccgt catcgcctcg cgcctcccag gaccgggcac catttatctg gcgcagaatc
      901 tcagcttccg ggcgccggta cggatcggcg acaaggtcga ggcaaaagtc acggtcatcg
      961 acatcgtccc tgaccgggcg cgcgtcatgc tcaagaccgt atgccgcgtc ggcgagacgg
     1021 tggtgatcga cggcgacgcg ctggtcatgg tgccggtgcg cggctaagct gcaacgtctc
     1081 tccctctacc cagagtctcc ggggccggat attttccgcc tgtcgagacc gagcaacccg
     1141 cgggcccatg cgtatcttcc gccacgacac cgtggccgcc gcagagcgcg gcgcagtggt
     1201 cgccatcggc aatttcgacg gcgtccatct cggccatcag gcggtgatcg gcgagaccgg
     1261 ccgcattgcc cgcgcccaaa acgcgcccct tgccgtgttg accttcgagc cccacccgcg
     1321 ccgcgttttc cggccggacg acccgccttt ccgcctcacg ccctttcgcg caaaggctca
     1381 ggccatcgag gcgctcggcg tcgacctgct cgtcagcttg catttcgatc acgccttcgc
     1441 ccagctcagt gcggaggatt tcatcgacca ggtgctggtc cgccatctcg gcgtccggca
     1501 tgtggcgatc ggttttgatt tcaccttcgg tcatgggcgg cgcggcacgg cgacgctgct
     1561 tcaggccgag ggacgggcgc gcggcttcgc cgtcaccgtg gtcgagccgg tgacagcggc
     1621 tggcggcgcg gtctattcgg cgacgcgcat ccgcgagcat ctcgtggccg gccgcccgcg
     1681 cgaagcagcc gcgctgcttg gccgcttctg ggagatcgag ggccgggtgg agcgcggcga
     1741 ccagcgtggg cgcggcatcg gatttccgac ggccaacctc gcgctcgacg attacttgcg
     1801 tccggcggcg ggcgtctatg cggtgcgcgc tgcggtcgag accgaaggag gagtgctctg
     1861 gcacgacgcc gtcgccaacc tcggccggcg cccgaccttc ggcggcagcg atcttcggct
     1921 cgaggtgcac ctcttcgatt tcgacgccga tctctatggc cgccatctcc gcgtcgcgct
     1981 catcgagcat ctgagacccg agcagaaatt cgccggcatc gacgcgctca aggcgcagat
     2041 tgccgccgac gcggcgcaag cgcgcgccat cctgaagacg acgcgggcgt tctgagacgg
     2101 gtcgctggcg cctccgccgc aacgccgcta ccatcgtcca cagggaagga aacaacgctc
     2161 gcgaggacgc tatggcctat gtccggggtc gcgtcattca tgacgcggat tcgcatatca
     2221 tggagctgcc gggatacctc gacggctatg tcgaggcgcg attcaagccg cagctgaacg
     2281 ccgacgcctc tttcgcccgg cgacccaagc tcgggtccca ggtgccggag atcctgcgcc
     2341 gccatgacga tccggtctgg cgtgcagacg acgacgcgca gatcatgctg cgcaagaact
     2401 gggacgccac cggagcgttt cgccgcgagg atcggccgaa ggcgctcgat catctcggat
     2461 tcgccagcca gctcgtcttc accacggcgc tcctcagcca catcaacaag ctcgagcatg
     2521 gcgacgacat cgatctcgcc tacgccgcag ccgccgcgca caaccggcat gtggtcgatt
     2581 tctgcgccgc cgatcgccgg ctgttggccg tgggctatgt gccgctcatg gatttcgcac
     2641 gcacccagga ggcggcgcgc caagcgatcg cgctcggctg caaggcgctg atgatcccgt
     2701 cgcaatgccc caaggcccat gccccgagcc atatcgggct cgatccgctg tgggcgcacg
     2761 cgcaggaagc agcattgccg atcatcttcc atgtcggcgg cggtggcccg ttggtatcgc
     2821 ccgccaactt cgtcaacggc ctgccgccgg tgcccgattt tcacggcggc gacgacaatt
     2881 tccgctcgat cgactacatg gcgatcagct atccggtgat gcaggcgctc gcaacgctca
     2941 tcctcgaccg ggtgctcgac cgcttccctc ggctcaagat cggcgtcatc gagcagggcg
     3001 catcctggct ccctggctgg atgcggtcca tggattccgc ttacctcgcc ttctacaaga
     3061 acgaggaacg cctgaaggcg atgtcgctga agccgagcga gttcgtgcgc cgccaggtgc
     3121 gcgtcacacc ctatccgcat gaggacacgg gctggaccat cgccaacagc ggtgaagaga
     3181 tctgcctctt ctcttccgac tacccgcatg tcgagggtgg ccgcaacccc ttgaagcgtt
     3241 tcgaggagag cctcgtcaac acgccggcct cggcgaagca gcgcttctat tgcgacaact
     3301 tcatcgatct catgggcgag ggtttgccgg ccgatttgcg ccatcccgtc ccatcggcgg
     3361 catgaggagc ctgagggcgt ccggttgacg ccggccgcgg ccctctccta tggtcggcgc
     3421 atggtcacgg ctcgacggcg gcagatagcg cgaattagcc gcccggtcct cgtctgagga
     3481 ccgggacctc gccgtctcgc cccctcgctg cgcccgttcc gacgcgcgcg ccctcgtaaa
     3541 agcgcaccgc catggccacc gactaccgct cgaccgtctt cctgccgcac accgattttc
     3601 cgatgaaggg cgacctgccc aagcgcgagc cgcagctgct cgagcgctgg agccggatcg
     3661 atctctggaa ccgccagcgc gcgctcttca aggggcggga gaaattcgtg ctccacgacg
     3721 ggccgcccta cgccaacggc aacatccatc tcggcaccgc cctcaacaag atcctcaagg
     3781 acgtggtgag ccgcgcccag aacatgctgg gcaaggactc ggtctatgtc ccgggctggg
     3841 actgccacgg cctgccgatc gagtggaaga tcgaagagca gtatcgcgcc aagaagaaat
     3901 ccaaggatga agttccgatc gtgcagttcc gcaaggaatg ccgcgacttc gccgcccact
     3961 gggtcgatgt gcagcgtgcc gagttccagc gcctcggcgt gatcggcgat tgggagcatc
     4021 cttacctcac catgagcttc gccgccgagg cgcagatcgc ccgcgagatc ggcaagttcc
     4081 tgatgaatgg cggcctctat cgcggctcga agccggtgat gtggtcggtc gtcgagcaga
     4141 ccgcgctggc cgaggccgag atcgagtatt acgaccacac ctcgccgacc atctgggtgc
     4201 gctttcccgt tgcgcagagc ccgcgtgccg agctcgcggg cgcgtcggtg gtcatctgga
     4261 cgacgacgcc ttggacgatc cccggcaacc gcgccatcgc ctatggcgcg gatttcgact
     4321 atgtgctggt ggaggtcacc gccgctgccg agggtagcct ggcgcgaccc ggcgagcgct
     4381 tcctcgtggt cgagccgctg gtcgtcgcca ccgcggaggt cgccggcatt actggccata
     4441 tccgcctggc gaagctcaaa ggcgcggatc tcgccggcac gatctgccgt cacccgctgc
     4501 acggccaggg ctacgacatc gcggtgccgc tgctgccggg gggcttcgtc accaccgagc
     4561 aaggcacggg cttcgtccat atcgcgccgg gccatggcgc cgacgactac gagctcggca
     4621 gccggcatgg gatcgaggtg ccgcagacgg tcgccgccga cggcagctac tacccgcatg
     4681 tgccgctctt cgccggcaag caggtctatc gcccgaacgg caagcccggc gacgccaatc
     4741 ccgccgtcat cgccgccttg cgcgacgccg gggcgctgct cgcggaaggc acgctcgtcc
     4801 attcctatcc ctgctcctgg cgctccaaag caccgctcat cttccggaat acgccgcaat
     4861 ggttcatcag catggagaag aaccagctgc ggcagaaggc gctcgccgcg attggcgaga
     4921 cccgatggat cccgccgcag ggcgagaacc gcatccgctc catgatcgag acgcgccccg
     4981 actgggtact ctcgcgccag cgcgcctggg gcgtgcccat cgtcgtcttc gtcgacaaga
     5041 agaccggcga gccgctgcgc gaccagagcg tcgtcgaccg catcgccgac gccttcgagc
     5101 gcgacggctc ggacgcctgg tacgcggccg atccccgcgt gtttctcggt cctggccgcg
     5161 atcccgccga ctacgagcag gtcaaggaca tcgtcgatgt ctggttcgat agcggctcga
     5221 ctcatgcctt cgtcctcgag aaacggtccg atctcaaatg gccggcatcg ctgtatctcg
     5281 agggctcgga ccagcatcgc ggctggttcc attcctcgct cctcgaggcc tgcggcaccc
     5341 gcgggcgcgc gccctatgaa gccgtcctga ctcacggctt cttcgtcgac gagcaggggc
     5401 gcaagatgtc gaagtccctc ggcaacacgg tcgcaccaca ggagatcgtg gcgaagagcg
     5461 gcgccgacat cctgcggctc tgggtggtga gctcggacta ttccgaggat ctgcgcatcg
     5521 ggccggagat cctgaaatat caagccgacg cctatcgccg gctgcgcaat acgctccgct
     5581 atctcctcgg cagtctcgcc ggcttcaccg aggcggagcg cgtgagcctc gacgccatgc
     5641 ccgagctcga gcgatgggtg ctgcatcgcc tccatgaact cgaccaggtg atccgtcgct
     5701 cgaccgaggc ctatgacttt cacgccatca tcacggccct ccacaatttc tgcgccgtcg
     5761 atctctcggc cttctatttc gacgtgcgca aggattcgct ttattgcgat ccgcctgctg
     5821 cgccgcggcg gcgaaccgcg cgcacggtgc tcgaccgtgt cttcgacagc ctcgcccgct
     5881 ggctcgcgcc gatgctgtgc ttcaccgccg aggaagcctg gatctgccgt cacggcgatg
     5941 cggccgagac gagcgtgcat ctcgtgacct atgccgagct gccggcgtcc tgggggaacg
     6001 acgcgctcgg tggcaaatgg gcggagatcc gcgagctgcg gcgcgtcgtc accggagcgc
     6061 ttgagctcga gcgcgcccag aagcgcatcg ggtccagcct ccaggccgcc gtcacggtct
     6121 acgcccagcc gcgccatgtc gcgctgctcg aagggctcga tctcgccgag atctgcatca
     6181 cctcttcggc aacgctgacc accgcgccgg caccggcgga caccttcacg ctacccgata
     6241 tcgccggaat cgccgtctct gtcgccgcgg cgccgggcga gaaatgtcag cgctgctggc
     6301 gcatcctgcc ggaagtaggg tcgttgtcgg cgcatcccga cctctgcgag cgctgcgccg
     6361 acgccgtcga ccgcctgggc gtcgcggtgg ccgactgatg ctgaagcgcg gtctggtggt
     6421 ggcggcgctg gtcgctctgg ccgatcagct cagcaaagcc tgggtgttgt cgttctttgc
     6481 cgagcccgga tccgggcttc acctgcaccc cgtcacgggg ttcttcaacc tggtgctcac
     6541 ctggaaccgt ggcgtcagtt tcgggctctt caacaatgac gacgcgatga atgccttggt
     6601 attcaccggc gccgcggcca tcattgtcgc aggtctcgtc ctgtggcttc accgcacgcg
     6661 cgaaccgctc ctcggcgttg ccatcggcct cgtcatcggc ggggcgatcg gtaatgtgat
     6721 cgaccgcata acgcgcggcg cggtcgtcga tttcctcgac tttcatctgg cgggctggca
     6781 ttttttcgcc ttcaatctgg cggattcggc gatcaccgtg ggcgtcttct tcatggtgat
     6841 cgacagcttg tttgcccgac gcgaagcgcc taattaggag gctggagtca gccatgtcca
     6901 cacggccgat cattctttcg ctctgcgcgt tggcgctcgc ctcgccgtcg ctgagcggtt
     6961 gcaccgatat gaagcgggca ttgggcatgg agaaggtggt gcccgacgag tttgcggtgg
     7021 tgcagggagc gcccctggcg atgccgcccg atttctctct gcgtccgccc aagcctggtg
     7081 caccccccag ccaggaagtc tcgccgaccg agcaggcgcg acaaaccatc ttccgggcgg
     7141 gcgatcaaca ggcggcggca gtgcccgatg ccgaccaact gagtcccgga gagaattcgc
     7201 tgctgcgcca ggccggcgcc gccaacgcgc cgcgcgacat ccgcgagacc gtcacccgcg
     7261 acgcgatgca gactcccccg ccgcccgatg agagcttcat cgacaagctg ctgttctggc
     7321 gcacgcccgg caagaccgcg aatgccgccg attcggtgct cgatccggcg gcagaagcgc
     7381 aacgacttaa agcgggtgga gcggccgcaa ccgggacagg aacaccggca gcgcccgtgc
     7441 cggagctgat tggcactccc accatcgagc gtcgtcgcga tccgtcgacg acgagctgac
     7501 gccgtccgag cagcggcgcc ggtcgcgcca aggaggatat cgtggcaaga ctggcgcctt
     7561 ggggcctcgt tctcgcattg ttgctcgcct gcggcacggc gcacgccgat acgggcaagc
     7621 agttcaacgc cgagactttt acgctcgcca acgggcttca ggtcgtcgtc gtcgagaacc
     7681 accgcgcgcc gattgcgaca cagatgatct ggtacaaggt cggctcggcg gacgagccgc
     7741 gcggcaagac cggcattgcg cattttctcg aacacttgat gttccggggc accaaggaaa
     7801 caccgcccgg cgccttctcg cgcatcgtcg cgcagaacgg cggccgggag aatgccttca
     7861 ctacggctga ctacaccggc ttcttccaga atgtggcggc ggaccggctc gacctggtga
     7921 tgcggctcga ggccgagcgc atggccgatc tcgtgatcaa cgacacggtg gttttgcccg
     7981 agcgcgaggt cattctcgaa gagcgccgca cgcgtatcga caacaatccg acggcgcttc
     8041 tcaacgagca ggtcaacgct gcgctctttc tccatcatcc ctatcgcaat ccgatcatcg
     8101 gctgggagca tgagatgcat gagctcggca ccgccgatgc gctcaccttc taccggacgt
     8161 ggtacgcgcc caacaatgcc gttctggtgg tggcgggcga tgtgacggtc gccggcgtgc
     8221 gcgcgctcgc cgagaaattt tacgggccga tcccgtcgcg cgctctcccc gagcgccatc
     8281 gcgtcggcga accaccgaaa gtggcggccg cgagccttgt catgaaaagc gcgcgtgtcg
     8341 gcgagccgac ctggagccgc tcttatctcg cgccgagcta tgccgctggc gccaagcaat
     8401 acgcctatgc gcttcaggtt ctcgccgaag tggtcggcgg cggctcgacc gcgcggctct
     8461 atcgtgcgct ggttctcgac aagggcttgg ccctcaatgc cggcagcttt tacaatccca
     8521 acgccctcga ttacggcgtc ttcggctggt atgcgacgcc gcagaagggc gtggcgatcg
     8581 ccgacctcga agccgccgtc gagggcgaga tcaagaaggt gctgacggac ggcgtgaccg
     8641 cggaggaagt cgagcgtgcc aagcgccgca tgcagcccca ggcggtttat gcgcgcgata
     8701 gcctcgatgg accggcgcgc gtcatcggcg aaggcctcgc caccgggcgc agcctggatg
     8761 agatcgagac ctggcctcaa cacatcggcg ccgtcactct cgacgacgtc aacgctgcgg
     8821 cccgatcggt gattcacgac gacacggccg tgacggccgt gctgctgccc gagccgacat
     8881 cgtgagcgag agagcgagca tgcatcgtcg cgttcgcgca cggcgcgtct tcttcactct
     8941 ctccccgttg acgggccgag aagaagaagt agcgctacga agaccctgcg ccatcctcgc
     9001 cttcgtctta gtcctcgtgc tggcgcttcc tgcacgggcg gcggaaaaga tccaagaggt
     9061 caccagcacc ggcggcatca cggcatggct ggtcgaggac cacagcctgc cggtggtgac
     9121 gctcgacgtg actttccgcg gcggcgccgc gctggacccc gcggacaaag ccgggcgcgc
     9181 gacgctgacg accgatcttc tcgacgaggg cgcgggcgac ctcgacagcc aggcttatca
     9241 gggccggatg gaggatcttg ccgcgtcgct ccaattcacg gctgcgccgg acggcatcag
     9301 cgcctcgttg cgcagcatca ccgccaatct agcgcccgcg ctcgagctct tgcggctctc
     9361 gctgaccgcg ccgcgcttcg acgaggcggc ggtcgcacgc gtccgcagcc agctcgtcgc
     9421 cgagctcgca cgcgatgtgc gcgagccgcg ctatatcgcc gaccggctgt ggtggcgcaa
     9481 cgcctatgac ggccatccct atgcgcagcc gctcaagggg accgcggaga gcatcgcacg
     9541 catcaccatc gccgatctgc aggccctggt gcatgatcgc tttgcgcggg atgtgatgct
     9601 gatcggtgtc gtcggcgacg tcacgcccga cgccctcaag acgctcctcg acaagacctt
     9661 cggcgctctc ccgccgcatg ctgcgccggg gatggtggcc gaggtgaccg cggcgccgaa
     9721 gaaggcgctg ctgttggcca agctgccgat tccgcaaagc gtcgtcgtct tcggcgaggc
     9781 ggggatcaag cgcgacgatc ccgactggta cccggccttt gtcgtcaacc acattttggg
     9841 cggtggcggc ttcaccgcgc ggctgaccgc ggaagtgcgc gagaagcgcg gacttgccta
     9901 ctccgtctat tcgacgctgg caccgcttcg ccacagcggc ctcattctcg gcggcgtcgc
     9961 gacgcagaac gcgcgtgttg cgcagtcgat cgagatcatt cgcgccgaat ggcggcgcat
    10021 gcgggacgaa ggaccgaccg ccgcggagct cgatgatgcc aagacctatc tcaccggctc
    10081 ctatcctttg agcctcgact cgacgggcag catcgccgcg acgctcgtcg ccatgcagcg
    10141 cgaccagctt gggatcgact atctcgagcg gcgtaatgcg ctgatcggag cggtcacgct
    10201 cgatgatgcc aagcgcgtcg cgcaccgcat ttttgatccc gacaagctga gctttgtggt
    10261 catcggctcg cccgaaggcc tcactggcgc cgaagaagtc gcccccagcg gcagctgacc
    10321 gcgatacgct tcgggcgtgg cgtagcgcgg cgctcggaga gtctgcagcg acgaaggagt
    10381 cgcgacccat gctctaccgc caggacgtaa ccggatgcct tgcggagacg gtgggcgcac
    10441 gcggcctcac ggcaagcgaa ctcgacgccg tgctggcccg tgcccgtcct gcgctcaagc
    10501 ggctcaaaga acggcgcggg ggtggcagcc tgcccctgtt gcagctgcct tcgcgtcgcg
    10561 acgatctggc accgctcgca gccatcgccg acgagttccg gcggcgtttc gatcatgtgg
    10621 tcgtgctggg catcggcggc tcgagtctcg gcggacaggc gctgcacgcg ctcgcggatc
    10681 aaggcttcgg accgcggccc ggcgcgccgc gcatccattt catggagaac atcgatccgg
    10741 cgaccttcgc ggcactgttc gccgctctcg atcccgcacg cacaggcgtc atagccattt
    10801 ccaaatcggg cagcacggca gagccctcgg cacagctcta tctttgtctc gactggctgc
    10861 gccaggcgct cggcaccggc gatctcgccg accgcgtgct cgtcattacc gagccccgcg
    10921 acaacccgtt gcgccgactc gccatggctc agaacatgcg catcctcgac cacgacacca
    10981 acatcggcgg tcgctacagc gttttgtcga atgtcggggt cttgccggcg ctcatcgccg
    11041 gcgtcgatgc gaccgcgttg cgtgagggtg cgacaatggc gctcgatgcg acgctctcgg
    11101 ctgccgagcc gcgcgaggcg ccggcggcac ttggcgcggc cgtctcggtc ggactggccc
    11161 ggcggcgcgg catcggtatc accgtgctga tgccttatgc cgaccgtctc gccgctttcg
    11221 gcctgtggtt ccgccagctc tgggcggaaa gcctgggcaa ggacggcaag ggaacgaccc
    11281 cgattcgcgc gctgggggcg gtcgaccagc acagccagct gcagctctat ctcgccggcc
    11341 ccgccgacaa gatgttcacc ctcgtgatgt tcgatgcggc cggcaacggt ggactcgtcc
    11401 cgcccgacct cgccagcgat cgcgacgtcg cctatctctc cgggcgacgg ttgggcgatc
    11461 tgctcgatgc cgagcagcgc gccaccgccg cgacgctgat cgcgcgcggt cgcccgactc
    11521 gcgttttcgc tctcgcgcga ctcgatgaag caagccttgg agcgctcctc atgcatttca
    11581 tgctggagac gatgatcgcc gccgaactct tcgaggtgaa tgccttcgac cagccggccg
    11641 tcgaggaagg caaggtgctc gcgcggcact acctcggtca aatgaagcgc tcctaagtga
    11701 tcccgcgacg gcggaggaat tctttccttc gtcgattcgc gatgatctcg ccgccgacaa
    11761 gcgctgcgcc gacgaggcgt tgcgtcgccg acgcgacgag cgctggcgaa agaggccgca
    11821 tccaggcaca atagatgcca tgaccatccg ccaactgccc ccggtcctcg tcaatcgcat
    11881 cgccgccggc gaagtggtcg agcggccggc ttcggcggta aaggagctgg tcgagaacgc
    11941 gatcgatgcc ggcgccacac gagtcgaggt cgtactgcgc gacggtggcc agagcttgat
    12001 cgccgttacc gatgacggcg gcggcatgac gcgcgcggag ctggcactcg cggtcgagcg
    12061 ccattgcacc tcgaaacttc ccgaggataa cctgctgagc atccgcagcc tgggtttccg
    12121 cggcgaggcc ctgccgtcaa ttggtgcggt gagtcggctc accatcacca gccgcccgcc
    12181 ggatgcggcc gaggcctggt cccttgctgt cgagggcggc gtcaaggccg cggcgcagcc
    12241 ggccgctcat ccggcgggca cgcgagtcga ggtgcgcgat ctcttctacg cgacaccggc
    12301 gcggctcaaa ttcatgaaga cggcgcgcag tgagcgcgat caggcgctcg ataccgtcga
    12361 gcgcctcgcc atggcctatc cggcgatcgc cttcatggtg atcggcgacg aggaccgggt
    12421 tctgctgcgc ctcaacgcgc tcggggcgga tctcgcgggc cacgacccgc aaacggcgcg
    12481 tcgcacccgg ctcgccgcaa ttctcggacg cgactttgcc gagaacgcgc tggcgatcga
    12541 cgccacgcgg gagaacatcc ggctcaccgg cctcgccggg ctgccgacgc tcaaccgcgc
    12601 caccgcccgc gaccagtatc ttttcgtcaa cggccgtccc gtccgcgaca agctgctggt
    12661 gggtgccgtg cgcggcgcgt atcaggactt cctggcgcgc gaccgccatc cgatggtggc
    12721 gttgttcgtc gactgccccg agcaggaggt cgacgtcaac gtccaccccg cgaaggccga
    12781 ggttcgcttc cgcgacaccg cgctcgtgcg cggcctcatc gtcggtgccc tccgccaggc
    12841 gctggctgcg gccggtcacc gcgcctcgac gacggtggcg gaggcggcgc tcgcggcctt
    12901 ccgcccggga gcagcgccgt cgggctccgg ctggcgccac gagtccggca cggcgatccc
    12961 ggctggtctt gcggaagcgg ccgcagccta tcacgcgccg ctgccggggc tcgcgatcga
    13021 tcgccccatg gcgcgtgccg cggaagccgt tgtcgatgac acggcgtcgg gtctctatcc
    13081 gctgggtgcc gcctgcgcac agctccacga gacctacatc gtcgcgcaga ccaacgatgg
    13141 gatcgtcatc gtcgaccagc acgccgccca tgagcgcctg gtctatgaac gcatgaaagc
    13201 ggcgctcgcg gggacggggg ttgcgcggca gatcctgctg ctgcccgagg tcgtcgagct
    13261 cgacgaaggc gcagcttcgc gactcgcggc acgacaggcc gagcttgccg agctcggcct
    13321 ggtgctcgag ctcttcggca ccggcgccgt cgtcgtgcgc gaaatgccgg ctctgatcgg
    13381 cgaggctgac atccaggggc tggtgcgtga tctcgccgac gagctggcag agctcggaga
    13441 cgcgctcgcc ctcaaggagc ggctcgagga agtctgcggc acgcttgcct gccatggcag
    13501 cgtccgcgcg ggacgccggc tcagccgcgc cgagatggat gcgctgctgc gccagatgga
    13561 ggcaacaccc catagcggcc aatgcaacca cgggcggccc acttacgtcg agctgagcct
    13621 tatcgacatc gaaaagctct ttgggcggcg gtagagcgca tctcgagcaa gtggaatcga
    13681 cgcttcataa catcgtcatt cccgcgaaag cggcaaccca ggggcgagcg acgtgccggt
    13741 ggccctggat cccgctttcg cggggatgac ggcaaaggaa tttgccccgt atttgcgcaa
    13801 tcgaggagcg actcgggacg ggtgatcgcc cgagacgcag atgagacggg gcgcgactcg
    13861 cttcccgccc cgcgagatct gtgacccagc tcacagacgc aaacgggccg gggccttagc
    13921 atcgcgccgc tcgtcatgat gaggactcgc gccatgcctc ggatcgccat cgttgttgcc
    13981 cgcaccgacg cgctctgcgc ccgcctcaat gtcggcctca tggccgttgc gttggtgctc
    14041 accatcctca ccggggcatt gtcggttgtg cgagccgcac aatgggtcga gtcccatgat
    14101 cccgaatggc tggtggcgac ggaccagtga tcgtcagctg ccgtcgcgcc gcaggaagac
    14161 gaatcgcgcg gcaccgtagc gacgctcctc gaggacggtg aatcccggcg gcagctccaa
    14221 agcctgccgc gccgacacct cgacgaccac gagcgcttcc ggcgccagcc agccgcaagc
    14281 ggcgagcgac gccagcgccg gtgtcgcgag ctcctcgcga tagggcggat cgagaaaggc
    14341 gaccgccgcc ggagcggcgg cacgcggcgg gcggcgtgca tcgcccgcga gcaccgtcga
    14401 ccgctccgcc tcgcccagcg ctgtgatatt gctgcgcaaa gcggcgagcg ccgtgcgatc
    14461 cttctcgatg aaggtggcgt ggcttgcgcc ccgcgacaac gcttcgagac cgaacgcgcc
    14521 ggtaccagcg aagacgtcga gcacgggcac cgcctcgaac accggccctt cggcaaagcg
    14581 gccatgggcg aggatgtcaa agagcccttt gcgcgcgcgg tcgctggtcg ggcggacggc
    14641 ctcgccgggc ggcgccgtaa ggcgccggcc cttatgccgg ccggcgatga tgcgcatgac
    14701 ggatagacgt ctcgtcgccg agttgctcgc ggagcacgcg ccgcggcact tcctcgaccg
    14761 cgcccctggg tagctcaccc agctggaagg ggccgtaggc gatgcggatg agccgcgtga
    14821 ccgcgaggcc gagatgctcc atgacgcgac ggatctcgcg gttcttgccc tcgcgcaagc
    14881 tgaccgtgag ccaggcgttg ctcgactgct ggcggtcgag catggcgcgg atcgagccat
    14941 aagcgacgcc gccgatggtg atgcccgctt cgagcgccgc gagccgcgcg ggatcgaccg
    15001 caccgtggac gcgcaagcgg taccggcgca accagccccg cgacggaagc tcgagagcgc
    15061 gcgcgagacc accgtcattg gtcaggagca acagcccttc agaattgaga tcgagccggc
    15121 ccaccgagat aacgcgcggc aactcgcccc gcagccggtc gaacaccgtg ggccgacctt
    15181 gcggatcgtg atgggtcgtg acgaggcctg gcggcttgtg atagcgccag agcctcgccg
    15241 gctcgtccga gggaagcggc ttgccgtcga cgcggatgtc gtcgcccggc atcaccgtca
    15301 gcgccggact gtccagcact ttgccgccaa ccgcgacgcg accctgcgcg atccagcgct
    15361 ccgcgtcgcg gcgcgagcat aacccggcgc gcgccaggcg cttggcgatg cgttcccctt
    15421 tttccggcat gctaacgcag cgctgctgca atgaatgcgg cgaagaggcg cgtgtcaccc
    15481 tcgctgatcg catattcggg atgccactgc accccgatca gaaagcggcg gcgcgggtct
    15541 tcgatacctt cgatcacacc gtccggcgcg acggcatcga cgacgaatcc cggcgccaga
    15601 tccttcaccg cctgatgatg ggcactgttg acgggaagct cggccaggcc ggtgatgcgg
    15661 cgcagcagcg tgccttccgc gatacgcacg acgtggcccg gctcggtgcg cgggttgggc
    15721 tgctcatggg cgagcgcgcc ctccacttca tcgggaatgt gctggatcag cgttccgcca
    15781 agcgcgacgt tgaggagttg ctggccgcca cagatgccga acaccgcctt gtcggctttc
    15841 acggcgccac gggtgatcgc cagctcgaag gtcgtgcgcc ggtccttggt ggttacactg
    15901 gcgtggcgcg tcgtcgcgcc gaacaatgcc gggtccacat cgaaggcgcc gccggtcacg
    15961 acgagtccgt cgatcaacgc cagataggcg tcggtctgct ccggctcatg cggaaggagg
    16021 atcggcagcc caccggctcg cgccacggcg gttgcgtagt tctcgcgaag cgcataccaa
    16081 ggatgcgtct tcgagtagcc gcccggcggc tcggaatcga gggtgagacc gatgacgggt
    16141 tgtgccatgc tgcggtcctg gggggtgcga cttgggcggc gatcctagag cctcggccgg
    16201 tgcatcgcca agagagcgtt gccgtgagcc atccccgcat cgtcgcgctc gcgctgccgg
    16261 tccgcaccga tgacatcgac ttcgccggcg tcgtcggcaa tatcgtctat gtgcgctggc
    16321 tcgaggatct gcggctcgcg atgctggcgg acgcctatcc gatcgcccga gcgctcagcg
    16381 acggggcggc gccgatcctc ctggagacgc aaatcgccta tgagcggccg gtgacgatcc
    16441 aagacgccgt cagcggacgc atgtcggtga ccgagatggg ccgcgtcaaa tggcatgtcg
    16501 ccgccgagtt cgcggtcggc gagcgcgtcc atgcgcatgc agcacaggtc ggaattttca
    16561 tccgcctcgc gacgcgccgc ccgatcgcgc cgccgctgcc gctgcgcgag cgcttcaggc
    16621 cggtcagcgg accgctgcag ggatgagcac cgagcggacg agcttcatgg cgctggcgct
    16681 cgccgaggcg gagcgtgccg gcgaacgcgg cgaggtcccc gtgggcgcgg tactggtcga
    16741 tggcgtgacc ggcgcggtgc tcgccgctgc cggcaaccgt gtcgaggcgc tcgccgatcc
    16801 gacctctcac gccgagatgc tggtcctgcg cgcgggtgcc gcggcgcgcg gtgccgcgcg
    16861 gctcgatgat tgcgatctct atgtaacgct cgagccctgc gcgatgtgcg ccgccgccat
    16921 cgccttcgcg cgcatccgtc gcctctattt cggcgcctat gaccccaagg gcggggcggt
    16981 cgagcacggc ccgcgcttct tcgaccagcc gacgtgccac caccgccccg aggtctatgg
    17041 cggcatcgag gagcggcgcg ccgcggaatt gctccgaaag tttttcaaag agaggcgctg
    17101 accgctggca tcatcccctc accctcccgc gcttcgcgcg ggcccctcct tctcccacaa
    17161 ggggagaggg aatattccgc tgcgtgagcg catgaatcag aattgctgcg ctggcagaga
    17221 cgttgagcga ttggacgcta ccggagccgg gaatggtcac gatcgcatcg caagcggcaa
    17281 gggtggcgcg cggcaggccg tcctcttcgt tgccgagcac cagggcaatc ggccgatcgc
    17341 tcctttccag cgggtcaagc ggcgtgcctt cgcgcgccgc ggtgccgagc acccgataca
    17401 atcggccaag atctttcagc gtctgagcga agcgcggcgc tcgatagagc gccaggtgct
    17461 cgagcccgcc ttcggcgacg cggtaggccg cgtcagaggg gcccgcctgc tccggatgat
    17521 cggacatcac caggcgcgac aagccgaaga aagccgcgct ccgagcgatc gcacccagat
    17581 tgtgcggatt gccgataccg tcgagcagca ataggagatc gccgtcacgc gcccagcgcg
    17641 ccgcttcggc ggcatcgagc atcgccacgg gtcgcggctg cgccagcgcg accacgccgc
    17701 cgtgaagcag ggtgccggcg acgcgctcga gctcctcggc gcccacgagg cgatagggct
    17761 tgcgagcgcg cgcgagcgtg gcgcagaacg tccccaacga tggcttcgca cgctcgtcga
    17821 agaagaggcg ctccacgcgc tccggcgccg tggcgaagag cgccgatacc gcgggcaggc
    17881 ccgcgatgcg caagaggtcg cgggcctcct tgcgcggccc cggaggatgc ggcgtccgcg
    17941 cctttttcac gacgggagcg cgcgccagcc gatgtcgcga cggtagaagc cctcgggcca
    18001 atcgatgcgc tcgatcgcgc cataggcttt ggcgcgcgct gccgccacgt ccttgcccaa
    18061 agccacaacg tcgagcacac gtcctccatc cgcaaggagc cggccgttca cgcgcttggt
    18121 gccggcatga aaaatcttga ccgttggatc gccgcgagtc gcgtccgcca ggccgcggat
    18181 ttcggtgtcg cgcagcggct cgccgggata gccctgagcg gcgagcacga cgcagaccga
    18241 gctttcggag tgccagcgca gatcgaaccc gtcgagcacc ccgtcgcgcg cggcgatgag
    18301 cgccggcaac agatcgctca tgaggcgcgg catcaaggcc tcgcattcgg gatcgccgaa
    18361 acggacgttg tactcgatga ggcgcgggcc ggccttggtg agcatcaagc cggcatagag
    18421 cagccccttg aagggacggc cttcggcggc catgcctttg accgtcggat tgatgaaccg
    18481 cgccatgatc tcgcgctcga gcgtggccgt gacgcagggc gcgggcgaat aggcccccat
    18541 cccgccggta ttgggcccgg tatcgccgtc gccgacgcgc ttgtggtcct gcgcagtggc
    18601 gagtggcaag gcgtggcgcc cgtccaccag cgcgaagaag ctggcctcct cgccttcgag
    18661 gaactcctcg acgaccactt ccgcacccgc ggcgccgaaa cggcgcgcga caagcgcgtc
    18721 gttgacggca gcaagcgcct cggccacggt catcgcaacg gtcacgcctt tgcccgccgc
    18781 aagaccatcg gctttgacga cgatcggcac accttgcttg cgcacgaact ccgcggcgct
    18841 cgcggcgtcg ccgaagcggc catacgccgc cgtcggaatc ccgtgccgca cgcagaaatc
    18901 tttggtgaag cccttcgaag actcgagcac cgatgcggca gcgctcggac caaaagcggc
    18961 gaccccggcc gcttcgaggc gatcgacgag gccggcgacc agcggcacct ccggaccaac
    19021 gacgacgaag tcgatgcgct cgcgccggca cagcgccagg atgccatcga catcgctcgc
    19081 cgcgaccggc acgcaagtcg cgccctcctc cgctattccc ggattgccgg gcgcggaaaa
    19141 gagcgcgtcg cacagaggcg aggcgacaat cgcccagcac agtgcatgct cgcggccgcc
    19201 cgagccgatg acgaggactc gcatatatct ctcctcggcg tttgctagtt gctcgacggc
    19261 acgagaacct tggccatcag gatctcgtca tagtagcgcc cattttgctt gagcgcgttt
    19321 ctcgcgattc cgtactcctc gaagccgacg ctggtataca agcgatgcgc ttgctcgttg
    19381 ccggcgacca ccgcggtgag gattcgttcg aggcgcgaag ctgctacggc cacgactgtt
    19441 tggcacagtt gccgcccgat gccgatgcgt cgggcctgcg gctcgacata cataccccac
    19501 aagaagccct tgtggttctc cttgagccct tgcgcgacca ggagcgccgc ggtcccgacg
    19561 agcgccccat cgacgaacgc gccaaatatg tcggaaccgg ttagccgatc tgcaaaccac
    19621 gttagtggct tcggatgctc ggattcgaaa gtgctcgaga aggcctcggg gctctgttgc
    19681 agcgcctcca atcgaatgtc gcgatagagg gccgcttccg tcgaagccaa ccggcggacg
    19741 cgcatctcag cggcgttcac acgggcatcc tttctccagg catgtaccag gttgcgacat
    19801 taccgaccgg ctgtctgatt tcccagccac ggccgcgtct tgtatcatag gcggcaatga
    19861 caggaa
//
LOCUS       DASYNS010000455         2810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3548932, whole genome shotgun sequence.
ACCESSION   DASYNS010000455 DASYNS010000000
VERSION     DASYNS010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548932"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="VGP50_14225"
     CDS             <1..1621
                     /locus_tag="VGP50_14225"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV8028587.1"
                     /translation="IRFQLLVACLIMTMISVLLGLFLHGTERNLADLAIRGYDKVFMA
                     MNYARNAQLAFDNVTRAYDGEHVAGSELDPELLDQLGRAMRHVHDHLAVAQERASSDT
                     SRAQAAALDAELKQVEEKLRSGDPAVDFVKIDAGFKNLVDTYASDGFRFRSDAEKAIA
                     ASETSTWAAMCTSMALAFLITLLLSRAIVPKLMRAVSIAEAIAAGRLDNEISARGGSE
                     TATLLRVLGRMQRAIADNIHRIESLHDEAVRSRTELKRHVEDALGKMAATVEDEMGAA
                     VGVVESGTTELADASTQMQGSSNALLNSARSLTDVADRSLGNAQQVAGAIEQLAASLQ
                     EIERQVTHSATIASTAVAGAAETRQVVAGLAEAAVKIGEIVDLISVIARQTNLLALNA
                     TIEAARAGEAGKGFAVVAAEVKNLANQTSQSSEDIRRQVAAIQESTTGAVAAIQRISQ
                     TIDSMNATAEGIASAVSEQSALNAAIARSVQETADGARAVAARTQEVSGEAERVGRLS
                     GLVGSASSALRQQIQVLRTTLVRAVSSGATP"
     gene            1898..2290
                     /locus_tag="VGP50_14230"
     CDS             1898..2290
                     /locus_tag="VGP50_14230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEV8028588.1"
                     /translation="MGRRGALGLGLAAASALAVGAGSSAEAATKGAKISVLYGAPKDP
                     AAFEKYYAEKHMPMVYAMKGIKRIELAKCLPQPDGKPPGFYRITELWFSSAKKMASVM
                     GTPEFKKIADDVPNFATGGATLLTSQIE"
     gene            2349..>2810
                     /locus_tag="VGP50_14235"
     CDS             2349..>2810
                     /locus_tag="VGP50_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028589.1"
                     /translation="MAGPEGAQRKLTMSDIRAAAERLKNWGRWGADDEIGTLNFTTPA
                     DIVAAARLVQKGKVMSLALAYDAKGPQGGKSKYPALGRFNPIHVMLRTGTDAYSGVLD
                     HRKIRGADDIIIMPLQCGTQWDGLGHIFYGDHMWNGYDCRTVTSAGAQKCGI"
BASE COUNT          510 a          940 c          960 g          400 t
ORIGIN      
        1 aattcgcttt cagctgctcg tcgcctgcct gatcatgacc atgatcagcg tgctgctcgg
       61 gctgtttctg cacggcaccg agcgcaatct cgccgatctc gccatccgcg gttacgacaa
      121 ggtgttcatg gcgatgaact acgcgcggaa cgcccagctc gccttcgaca acgtcacccg
      181 cgcctatgat ggcgagcatg tggccgggag cgagctcgat cccgagctcc tcgaccagct
      241 cggccgcgcc atgcgccacg tccatgacca cctcgccgtg gcccaggagc gcgcatcgag
      301 cgatacgagc cgcgcgcagg cggcggcgct cgatgcggag ctcaagcagg tcgaggagaa
      361 gctccgcagc ggcgatcccg ccgttgattt cgtcaagatc gatgccggct tcaagaacct
      421 cgtcgacacc tacgcctcgg acggattccg cttccgcagc gatgccgaga aggcgatcgc
      481 cgccagcgag acctcgacct gggccgccat gtgcacctcg atggcgcttg cctttctcat
      541 caccttgctg ctgagccggg cgatcgtgcc gaagctgatg cgcgccgtga gcatcgccga
      601 ggccattgcg gccggccgtc tcgacaacga gatcagcgcc cgcggcggca gcgagaccgc
      661 gacgctgctg cgcgtgctgg gccgcatgca gcgcgcgatc gccgacaaca tccatcgcat
      721 cgagagcctg cacgacgaag cggtgcgctc gcgcaccgag ctcaagcgcc atgtcgagga
      781 cgctctcggc aagatggccg ctacggtcga ggacgagatg ggcgccgccg tcggcgtcgt
      841 cgagagcggc acgaccgagc ttgccgacgc ctcgacgcag atgcagggca gctccaacgc
      901 gcttttgaac agcgctcgat cgctgaccga tgtggcggat cgctcgctcg gcaacgcgca
      961 gcaggtggcg ggcgccatcg agcagctcgc ggcctcgctc caggagatcg agcgccaggt
     1021 cacgcattcc gcgacgatcg ccagcaccgc cgtcgccggg gcggcagaga cgcgccaggt
     1081 cgtggctggt ctcgccgagg cggcggtgaa gatcggcgag atcgtcgacc tcatcagcgt
     1141 catcgcccgc cagaccaacc tgctggcgct caacgcgacc atcgaggcgg cgcgcgcggg
     1201 cgaagccggc aagggcttcg ccgtcgtcgc tgccgaggtc aagaacctcg caaaccagac
     1261 gtcgcagtcg agcgaggaca tcaggcggca ggtggcggcc attcaagaat cgacgacggg
     1321 cgcggttgcc gcgatccagc gcatctcgca gaccatcgac agcatgaacg cgaccgcgga
     1381 agggatcgcc tcggcggtga gcgagcagag tgcgctcaat gccgcgatcg cgcgaagcgt
     1441 gcaagagacg gccgacggcg cccgcgcggt ggcggcgcgc acccaggagg tgtcgggaga
     1501 ggccgagcgc gtcggccgcc tctcgggcct cgtcggcagc gcgtcgagcg cgctccgcca
     1561 gcagatccag gtcctgcgca cgacgctggt gcgcgccgtc agctccggcg caacgcccta
     1621 ggcgaacgcg caaagcgcgt tcgtccgcgg cggcgccagc gattgcgtag caatcgcgag
     1681 agccgcaccg agcgggtgat gtaccattgc ttgcaccgtg cgggctctcg cggttgcaag
     1741 agcaaccgct gccgcccgcc tgcgcgacgc ccgcgacgtc gcgggcgtcg cgctttgcgc
     1801 gttcgtgtcg gctgtgccaa catcgcgccg ccgaacaagg ccgctatccc gcggccgtgg
     1861 catcgaggga gggaacagca tgagcaggaa agcggtgatg ggccggcgcg gcgctttggg
     1921 tcttggtctc gcggcggcat cggcgctggc ggtgggggcg ggctccagcg ccgaggccgc
     1981 gacgaagggc gccaagatca gcgtgcttta cggcgcgccg aaggatccgg cagcgttcga
     2041 gaaatactat gctgagaaac acatgccgat ggtctacgcg atgaagggga tcaagcgcat
     2101 cgagctggcg aagtgcctgc cacagccgga tggcaagccg cccgggttct accgcatcac
     2161 cgaattgtgg ttcagcagcg cgaagaagat ggcgagcgtc atgggaacgc cggagttcaa
     2221 gaagatcgcc gacgacgtgc caaatttcgc gacgggcggc gccaccctgc tgacgtcgca
     2281 aatcgagtag cccgcgtccc gccaccgcct gtcggcgcgg aagcgcagcg aaaaaatcgg
     2341 aggagagcat ggccggaccg gaaggcgccc agcgcaaact caccatgagc gacatccgcg
     2401 cggcggcgga gcggctcaag aattgggggc gctggggcgc cgatgacgag atcgggacgc
     2461 tcaacttcac gacgcccgcc gacatcgtcg ccgcggcgcg gctggtgcag aagggcaagg
     2521 tgatgtcgct ggccttggct tacgacgcca aggggccaca gggcgggaaa tcgaaatatc
     2581 ccgccctcgg ccgctttaat cccatccatg tcatgctgcg caccggcacc gatgcctatt
     2641 cgggagtgct cgaccatcgc aagatccgcg gcgccgacga catcatcatc atgccgctgc
     2701 aatgcggcac gcaatgggat gggctcggcc acatcttcta tggcgatcat atgtggaacg
     2761 gctacgactg ccgcaccgtc acctcggccg gtgcccagaa atgcggcatc
//
LOCUS       DASYNS010000456        10172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2300725, whole genome shotgun sequence.
ACCESSION   DASYNS010000456 DASYNS010000000
VERSION     DASYNS010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10172
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2300725"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /gene="oxc"
                     /locus_tag="VGP50_14240"
     CDS             complement(<1..1589)
                     /gene="oxc"
                     /locus_tag="VGP50_14240"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEV8028590.1"
                     /translation="MTQARKLKEPASTAADDTQELTDGFHLVIEALKLNGLTTIYGVP
                     GIPITDLGRMAQAAGIRVLSFRHEQSAGYAAAIAGFLTKKPGLCLTVSAPGFLNGLTA
                     LAHATTNCFPMILISGSSEREVVDLQQGDYEEMDQLAVARPLAKAAFRVLGAADIGIG
                     VARAIRAAVSGRPGGVYLDLPAKLLSQVMNAEAGRKSLVKVIDAAPAQIPAPSAIKRA
                     LDVLKSAKRPLIILGKGAAYAQADDAIRTLIEKSGIPFLPMSMAKGLLPDTHPQCAGA
                     ARSTVLKDCDVAMLIGARLNWLLSHGKGKTWGEAPKKFIQIDIEPREMDSNVEIAAPV
                     VGDIGSCVAALVEGMGGNWPAAPPEWIDGVRAKREENVVKMASRLMNNSTPMDFHGAL
                     GALRGVIKERPDTILVNEGANTLDLARGVIDMYKPRKRLDVGTWGVMGVGMGQAIAAA
                     VETGQPVLAVEGDSAFGFCGMEVETICRYGLPVCIVVFNNGGIYRGTDANPGGSDPAT
                     TVFVKGARYEKIIEAFGGAGV"
     gene            complement(1621..2313)
                     /locus_tag="VGP50_14245"
     CDS             complement(1621..2313)
                     /locus_tag="VGP50_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV8028591.1"
                     /translation="MAQRLAVQPIDTQSSFRTQAYHALKRAITSVNIYAQEGDLRLDE
                     RQLSQDLGVSRTPIREAMSLLEREGFVKTLPRRGIFVVRKTKKEIIEMIQVWAALESL
                     AARLITLNASDADIAGLRHLFDEFRGAGHSPAEQLDEYSDANINFHQSIIRLAGNRLI
                     ADMTENLFIHVRAIRQMTIGQSNRAARSIVDHLNIIEALERRDTARAEELARRHTLDL
                     AAHVEKHGDFLS"
     gene            2621..5980
                     /locus_tag="VGP50_14250"
     CDS             2621..5980
                     /locus_tag="VGP50_14250"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8028592.1"
                     /translation="MKSSAAAPAGERERLGALERFRILDTEPEERFDRVARLAARLFR
                     TPIALVTFLDGHRQWHKARVGITRTEIPREAAFCDHTIRGDRALIVADARNDARFAEN
                     PVVRQGIAFYAGAPLITADGHRIGTVCVLDRKPRPDIGAEDGEQLALLAAMVVDELEL
                     CVAERRLSAELASRSIAEERLKLLNDLTEAAAAAPDFKTAMRACLELVAEHAGADCAV
                     AYGLALDAARLELAAEYAANAQGEAFLDLLRGFAADGDDSPAGESVRAQRLVAIIDLA
                     GAEHRHATLARAAAEHGFRVWLGIPFEDGGAKFSLTFLFRQLPRDMARLAETIHGLSG
                     KLRDLLARKQAEERIALLQSVVLHATDAVMIMESAVGKGGPPPIIYVNRAFSAMTGYG
                     TEDVLGATPELLFGPRTSADAIRHLLAAFSHAEPVRFELVNYRRDGSHFWAEADLAPM
                     RDADGIHTHWILTLRDTTARKHAEEQLRERELTLRQLALQQAAILDALPAHVAVLDRT
                     GRILSVSRSWQDFATAAGIASGESVGDSYFTFCAAECWGGVGLVIADGLSAVLAGRQT
                     QFSIDHPCDQRGWRRWYRLMAAPLSTGQSAGAVVMHLDVTASKLAEEALRREKEFSEF
                     LIKSSSEGIVVFDRDFSITLWNPGIERVTGMSVDEVLGRRIFEVLPYLVDTASETAMR
                     AALGGRETSLYDQRYAVPGTGREGFYDAFFSPLYSRGRDVIGGIGFLRETTERRRVED
                     ALRQSQKMEAVGQLTGGIAHDFNNMLTVIAGNLELLEGKLAQEPRLLRLVGSATLAAS
                     RAEKLTQQLLTFSRRQQLRPQPIDFNQIVIGMDDLLHRTVGEHIEIRAQLAPELWPAL
                     ADPNQIETALLNLILNARDAMTGGGHITIETANAELAVAQGEISAGVYATLAVTDTGQ
                     GMSEEVLAHVFEPFFTTKEVGKGTGLGLSQVYGFVKQSTGHVKIESNPRVGTTVRLYL
                     PRAPGSAGVAGATPTGERQYRGDECVLVVEDDHGVRDFAVSVLREFGYHVLEAPNGEA
                     ALELLAATAGIDLLFTDVVMPGKLTGADLAKAALERRPGLRLLFTSGYTTRLVEKEWP
                     ENTVDLLRKPYRSVDLAARVRAVLDRSAAAE"
     gene            6128..7549
                     /locus_tag="VGP50_14255"
     CDS             6128..7549
                     /locus_tag="VGP50_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEV8028593.1"
                     /translation="MSIAKKKHVALRGRLVIVGFGSIGQGVLPLLLRHLELAPEQITI
                     VTAEERGHAEAEELGVRFLVKPLTRDNYVTVLDPLLGKDDFLLNVSVDVSSVALIELC
                     RKKGALYLDTCIEPWPGGYTDPALTPSLRSNYALRESALALRADKGKAPTAVVTHGAN
                     PGFVSHLVKEALLNIAMDTGATVATPKTRAEWGALAEKLGIKVIHIAERDTQTSPRPK
                     RPGEFVNTWSIDGFVGEGQQPSELGWGSHERHFPADGSRHDFGPGGAIFLSRPGASTR
                     VRSWTPLEGPYHGFLITHGEAISIADYLTVRDGDQARYRPTVHYAYHPCDSAVLSLHE
                     FAGKNWRLQGQKRLLVDEISAGIDELGVLLMGHARGAYWYGSQLSIEEARRLAPYNNA
                     TSLQVTAAVLAGVVWAIENPQAGIVEPDEMDFTRILKIMRPYLGKIVGAYSDWTPLRD
                     RARLFPEDIDQDDPWQFKNFRVV"
     gene            7637..8368
                     /locus_tag="VGP50_14260"
     CDS             7637..8368
                     /locus_tag="VGP50_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEV8028594.1"
                     /translation="MSAKGWLLALMVALIAGGAAPAAARPVDLALVLAVDVSGSVDAE
                     RYELQRHGYAEAFASNEVIAAIAAGENHAIVVTLVEWSGAAHQQQMIAWTLIHDAASA
                     EAFGSAIAETPRAFSDWTSISAALDYAVALFADAEVEAARRVIDISGDGVNNNGRSII
                     EARDAAIAAGITVNGLPILSEYPTLDGYYRENVIGGPGAFAVAVNDFQGFGAAILSKL
                     VREIAGTQGPSVVRFAGRARGRNPG"
     gene            8393..9094
                     /locus_tag="VGP50_14265"
     CDS             8393..9094
                     /locus_tag="VGP50_14265"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEV8028595.1"
                     /translation="MLGSASSGKKRLPRLAGIAAALLLAAGVACADEALPPIWQMVEK
                     RPPGPLPGEPVLGKADTPLTIIEYASLTCPHCARFATQTLPKLKQEWIDTGKAKLVFR
                     DFPLDGLALRAAALTHCVPPERYLEFVDTLFKEQPSWIVLGDKAIARIASLGGLSEAQ
                     AEACLADTKRVESISAARAAAEKEWGVDATPTFFIDGMKISGAYPYVDLESKDRDFKS
                     ALATALAQKQPSDAH"
     gene            complement(9091..9948)
                     /locus_tag="VGP50_14270"
     CDS             complement(9091..9948)
                     /locus_tag="VGP50_14270"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEV8028596.1"
                     /translation="MIVAPLGRVLLAALLLGTAQARAEEQEHLVLTSDTILHDQPWLL
                     AQEIEAVASHRRGAPDLYFLGFAGYAGQDVFMKEVETVRTLFDERFGTRGRSLLLVNN
                     LDTVKELPVASAGNLRLALDVIAAKMNLDNDVLFLFLTSHGAENELAVSFPPLQLEDI
                     APQDLRHMLDAAGIRWRVIVISACYSGSFVDALKDRQSLIITAAARDRSSFGCSNEND
                     FTYFGDAYFNQALRHGSSFIAAFDEAQRLIASREQEEKLTPSEPQIYIGGAIKEKLAH
                     LGQDAPRDH"
     gene            complement(9966..>10172)
                     /locus_tag="VGP50_14275"
     CDS             complement(9966..>10172)
                     /locus_tag="VGP50_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV8028597.1"
                     /translation="NSCPHLGTPLDFLPDRFLDRDGRHLLCSTHGARFRIADGFCIDG
                     PCAGKSLERIGLRVEGAEILMVTP"
BASE COUNT         1647 a         3448 c         3384 g         1693 t
ORIGIN      
        1 tgcacgccgg cgccgccgaa ggcttcgatg atcttctcat agcgcgcgcc tttgacgaag
       61 acggtcgtcg ccggatccga tccgcccgga ttggcgtcgg tgccgcggta gatgccgcca
      121 ttgttgaaga cgacgatgca gaccggcagc ccgtagcggc agatcgtctc cacctccatg
      181 ccgcagaaac cgaaggcgct gtcgccttcg acggcgagca ccggctggcc ggtctcgacc
      241 gccgcggcga tcgcctggcc catgccgaca cccatcacgc cccaggtccc gacgtcgagg
      301 cgcttcctcg gcttgtacat gtcgatgacg ccgcgcgcga gatcgagcgt gttggcgcct
      361 tcgttgacga ggatcgtgtc cggccgctcc ttgatgacgc cgcgcagggc gccgagcgcg
      421 ccgtgaaaat ccatgggcgt gctgttgttc atgaggcgcg acgccatctt gacgacattt
      481 tcctcgcgct tcgccctgac gccgtcgatc cactcgggcg gcgccgccgg ccagttgccg
      541 cccatgccct cgaccagcgc ggcgacgcag gaaccgatat cgcccaccac cggtgcggcg
      601 atctcgacat tgctgtccat ttccctgggc tcgatgtcga tctggatgaa tttcttcggc
      661 gcctcgcccc aggtcttgcc cttgccatgc gacaggagcc agttgaggcg cgcgccgatc
      721 agcatcgcga cgtcgcaatc cttcaacacc gtcgagcgcg cggcgccggc gcattgcgga
      781 tgcgtgtccg gcaacaggcc tttggccatg ctcatgggca ggaagggaat gccgctcttc
      841 tcgatcagcg tccggatcgc gtcgtcggcc tgcgcgtagg cggcaccctt gccgaggatg
      901 atcagcggcc gcttggcaga cttgagaaca tcgagcgcgc gtttgatggc gctgggcgcc
      961 gggatctgcg ccggcgccgc atcgatgacc ttcaccagcg atttccggcc ggcctccgcg
     1021 ttcatgacct gactgagcag cttggccggc agatcgaggt agacgccgcc cggccgcccc
     1081 gagacggcgg cgcggatcgc gcgcgcaacg ccgataccga tgtcggcggc gccgaggacg
     1141 cggaacgccg ccttcgcgag cggccgcgcg acggcgagct ggtccatctc ctcataatcg
     1201 ccctgttgca ggtcgacgac ctcgcgctcg gacgagcccg agatcaggat catcgggaag
     1261 cagttggtcg tcgcatgggc gagggccgtc aggccattga gaaagccggg cgcggacacc
     1321 gtaaggcaaa gccccggctt cttagtgagg aagccggcga tggcggcggc gtagccggca
     1381 ctctgctcgt ggcggaatga gagcacgcgg atgcccgccg cctgggccat gcggcccaga
     1441 tcggtgatcg ggatgccagg cacgccgtag atcgtcgtaa ggccgttgag cttgagcgcc
     1501 tcgatgacga gatgaaatcc gtcggtcagc tcctgggtgt cgtccgccgc ggtgctcgcc
     1561 ggttctttga gcttcctggc ttgcgtcatc aggtctgttc tcctccggcc gtctcgttgc
     1621 tcaactcagg aaatcgccgt gcttttcgac atgcgcggcg agatcgagcg tgtgccggcg
     1681 cgcgagctcc tccgctcggg ccgtatcgcg ccgctccagc gcctcgatga tgttcagatg
     1741 atcgacgatc gagcgcgctg cgcgattgct ctgcccgatc gtcatctggc ggatggcgcg
     1801 gacatggatg aacaggttct ccgtcatgtc ggcgatcagg cggttgccgg cgagacggat
     1861 gatcgactgg tggaaattga tgttggcgtc ggaatactcg tcgagctgct ccgcgggcga
     1921 atgcccggcg cccctgaact cgtcgaagag gtggcgcagg ccggcgatat ccgcgtccga
     1981 ggcgttgagg gtgatgaggc gggccgccag gctttcgagc gccgcccaga cctggatcat
     2041 ctcgatgatc tctttcttgg tcttgcgcac gacgaagatg ccgcgccgcg gcagcgtctt
     2101 gacgaagcct tcccgctcca ggagcgacat tgcctcgcgg atcggtgtgc ggctcacccc
     2161 cagatcctgc gagagctggc gctcgtcgag ccgcagatcg ccctcctgcg catagatgtt
     2221 gaccgaggtg atggcccgct tcagcgcatg ataggcctgg gtgcggaagc tcgattgcgt
     2281 gtcgatcggc tggacagcga gccgttgcgc cacgatgttc tcccgttccg ttctgggata
     2341 cgaggatatt gtatctgata tatcaggtca aggaggagcc ggtcatccaa ggcttggtag
     2401 caaccggcgg atgcgcaggg caccggtggt tttcgccgat cacgacgggt atcgtcagga
     2461 agtacggctt ccgacgcacc atcaaccgag cgtaagctcg cgcagattcc cgtcgtccca
     2521 gtccttcgcg tcgcaggctc gcggcgcgct gtcgcagtgg tgcttgtttc cctgacaggg
     2581 cggcgtttgt tgcgcgatgc ggcaggcggg gagccgaagg atgaagagtt cagcggcggc
     2641 accagcaggt gaacgggagc gtctcggcgc gctcgagcgc ttccgcatcc tcgataccga
     2701 gcccgaagag cggttcgatc gcgtggcgcg ccttgccgcg cggcttttcc ggacgcccat
     2761 cgccctcgtc accttcctcg acggccatcg ccagtggcat aaggcacggg tcggcatcac
     2821 gcgcaccgaa atcccgcgcg aggccgcctt ctgcgaccac acgatccggg gcgaccgcgc
     2881 cctcatcgtc gccgatgcgc gcaacgacgc gcgctttgcc gagaacccgg tggtgcgcca
     2941 aggcatcgcc ttctacgccg gggcaccgct catcaccgcc gatggccacc gcatcggcac
     3001 cgtttgcgtg ctcgaccgca agccgcggcc cgatatcggc gcggaggatg gcgagcaatt
     3061 ggcgttgctc gccgcgatgg tcgtcgacga actcgagctg tgcgtcgccg agcggcggct
     3121 cagcgccgag ctcgcctcgc ggagcatcgc cgaggaacgg ctcaagctcc tcaacgatct
     3181 gaccgaggcc gccgctgccg cccccgactt caagaccgcg atgcgcgcct gcctcgagct
     3241 cgtcgccgag cacgccggcg ccgattgcgc cgtcgcttac gggctcgcgc tcgacgccgc
     3301 gcggctcgag cttgcggcgg agtatgccgc caacgcccag ggggaagctt tcctcgattt
     3361 gctgcgcggc tttgccgccg acggcgacga ttcgcccgcc ggcgaatcgg tccgcgcaca
     3421 gcgcctcgtc gcgatcatcg atctcgccgg cgcggagcat cggcacgcga cgctcgcccg
     3481 cgccgccgcc gagcacggct tccgcgtctg gctcggcatc cccttcgagg atggcggcgc
     3541 gaagttcagc ttgacgttcc tcttccggca gttgccgcgc gacatggccc gcctggccga
     3601 gaccatccat ggtctctcgg gcaaattgcg cgacctcctg gcgcgcaagc aggcggagga
     3661 gcgcatcgcg ctcctgcaat cggtggtgct gcatgccacc gacgcggtca tgatcatgga
     3721 aagcgcggtc ggcaagggcg gcccgccgcc catcatctat gtcaaccgcg ccttcagcgc
     3781 catgaccggc tatggcaccg aggacgtgct gggcgcgacg cccgagctgc tgttcgggcc
     3841 gcggaccagc gccgacgcca tccggcacct gctcgcggcg ttcagccacg ccgagccggt
     3901 gcgcttcgag ctcgtcaact accgccgcga cggcagccat ttctgggccg aggccgatct
     3961 ggcgccgatg cgcgacgccg acggcatcca cacccattgg attctgacgc tccgcgacac
     4021 caccgcgcgc aagcacgccg aggagcagct gcgcgagcgc gagctgacgc tgcggcagct
     4081 cgcgctccag caggcggcca tcctcgacgc attgcccgcg catgtggccg tgctcgaccg
     4141 cactgggcgg atcctgtcgg tgagccgcag ctggcaggac ttcgccaccg ccgccggcat
     4201 cgcgtcgggc gagtcggtgg gcgacagcta tttcactttc tgtgccgccg aatgctgggg
     4261 cggcgtcggg ctggtgatcg ccgacggctt gagcgccgtt ctcgccggac ggcagaccca
     4321 gttcagcatc gaccatcctt gcgaccagcg cggctggcgg cgctggtacc ggctgatggc
     4381 ggcgccgctc tcgaccggtc agtcggcggg cgcggtggtc atgcatctcg atgtcaccgc
     4441 cagcaagctc gccgaggagg cgcttcgccg cgagaaggag ttctccgagt tcctcatcaa
     4501 gagcagctcg gagggcatcg tcgtcttcga ccgcgacttc agcatcactc tctggaaccc
     4561 gggaatcgag cgggtcaccg gcatgagcgt cgacgaggtg ctgggccggc ggatcttcga
     4621 ggttctgccc tatctcgtcg acacggcgag cgagacggcg atgcgcgccg cgctcggcgg
     4681 gcgcgagacc tcgctctacg accagcgcta cgccgtcccc ggcaccgggc gcgaaggctt
     4741 ttacgatgct ttcttctcgc cgctctacag ccgtggccgc gacgtcatcg gcggcatcgg
     4801 cttcctgcgc gagacaaccg agcgccgccg cgtagaggac gcgctccggc aatcgcagaa
     4861 gatggaagcg gtgggtcagc tcaccggcgg catcgcccat gacttcaaca acatgctgac
     4921 ggtcatcgcc ggcaatctcg agctgctcga aggcaagctc gcgcaggagc cgcggctcct
     4981 ccgcctcgtc ggctcggcga cgctcgcggc aagccgcgcc gagaagctca cccagcagct
     5041 cctcaccttc tcgcgccgcc agcaattgcg gccgcagccg atcgacttca accagatcgt
     5101 catcggcatg gacgacctgc tccaccgcac ggtgggcgag catatcgaga ttcgcgcgca
     5161 gctcgcgccc gagctctggc cggcactcgc cgaccccaac cagatcgaga cggcgctgct
     5221 caatctgatc ctgaacgcgc gcgacgccat gacgggcggc ggccacatta cgatcgagac
     5281 cgccaatgcc gaactcgcag tcgcgcaggg cgaaatatcc gccggcgtct atgcgacgct
     5341 tgccgtcacc gatacgggcc aaggcatgtc cgaggaggtg ctggcgcatg tcttcgagcc
     5401 cttcttcacg acgaaggaag tgggcaaagg caccggcctc ggcctgtcgc aggtctacgg
     5461 tttcgtcaag cagtcgaccg gccacgtcaa gatcgagagc aacccgcgcg tcggcacgac
     5521 ggtgcgcctc tatctgccgc gcgctccggg atcggccggg gtcgccggcg cgacgcccac
     5581 cggcgagcgg cagtaccgcg gcgacgaatg cgtgctggtg gtcgaggacg atcacggcgt
     5641 gcgcgatttc gccgtgtcgg tgttgcgcga gttcggctac cacgtgctcg aggcccccaa
     5701 tggcgaggcg gcactcgagc tccttgccgc cacggccggc atcgacctcc tcttcaccga
     5761 cgtcgtgatg ccggggaagc tgaccggcgc cgatctcgcc aaggcggcgc tggagcggcg
     5821 gcccggcctc cggctgctgt tcacctcggg ctacacgacg cggctggtcg agaaggagtg
     5881 gccggagaac accgtcgacc tcttgcgcaa gccctaccgc tcggtcgatc tcgccgcccg
     5941 cgtccgcgcc gtgctcgacc gctccgccgc cgccgaataa cttcgtcggc agagtgtggc
     6001 gtcggtcttg cagacacctc ttctcgtctt aactatgagt ccctgttcag cgcgcttgac
     6061 aggaagggag cgcgagcata cgcgttgtcg aaacgcgctc ctccagacga gacgagacga
     6121 ggcgagcatg tccatcgcga agaagaagca tgtcgccctt cgcgggcgac tggtcattgt
     6181 cggcttcggc agcatcggcc agggcgttct gcccttgctg ctgcgtcacc tcgagctggc
     6241 acccgagcag atcaccatcg tcaccgccga ggagcgcggc cacgccgagg ccgaagagct
     6301 tggcgtccgc ttcctcgtga agccgctcac ccgcgacaac tacgtaacgg tgctggaccc
     6361 gctgctgggc aaggacgatt ttctgctcaa cgtctcggtc gacgtctcga gcgtggcgct
     6421 catcgagctc tgccgtaaga agggcgcgct ctatctcgac acctgcatcg agccttggcc
     6481 cggcggctat accgatcccg cgctcacgcc gtcgctgcgc tcaaactacg cgctgcgcga
     6541 gagcgcgctc gcgctccgag ctgacaaagg aaaggcgccg accgccgtcg tgacgcatgg
     6601 cgccaaccca ggcttcgtct cgcatctcgt caaggaggcg ctgctcaaca tcgccatgga
     6661 caccggcgcg acggtcgcca cgcccaagac tcgcgccgag tggggcgcgc ttgccgaaaa
     6721 attgggcatc aaggtgattc atatcgccga gcgcgacacg cagacctcgc cccggcccaa
     6781 gcgtcccggc gagttcgtca acacctggtc gatcgacggc ttcgtcggcg aaggccagca
     6841 gccatccgag ctcggctggg gcagccatga gcggcacttc cccgccgatg gtagccgcca
     6901 cgatttcggc cccggcggcg cgatctttct gagccgcccg ggagcatcga cgcgcgtgcg
     6961 cagctggacg ccgctcgaag gcccctatca cggcttcctc atcacgcatg gcgaggcgat
     7021 ctcgatcgcc gattacctga cggtgcggga cggcgaccag gcgcgctacc gcccgaccgt
     7081 gcactatgcc taccaccctt gcgatagcgc agtgctgtcg ctgcacgaat tcgcgggcaa
     7141 gaactggcgg ctccagggcc agaaacgcct cttggtcgac gagatcagcg cgggcatcga
     7201 tgagctgggc gtgctcctca tgggtcacgc gcggggcgcc tattggtatg gctcgcagct
     7261 ctcgatcgag gaggcgcgcc ggctcgcgcc ctacaacaac gccaccagcc tgcaggtgac
     7321 ggccgccgtg ctggcgggcg tcgtctgggc gatcgagaac ccgcaggccg ggatcgtcga
     7381 gccggacgag atggatttca ccaggatcct caagatcatg cggccctatc tcggcaagat
     7441 cgtcggcgcc tacagcgact ggacgccgct cagggaccgt gcccgcctct tccccgagga
     7501 catcgaccag gacgacccct ggcagttcaa gaactttcgg gtcgtctgac ggcacgaaag
     7561 gtgcagccgc tcgccgtcgt gaacctctcg cctgccgccg ggcgttatcc gctggtaggc
     7621 gagggaggcg gcaaggatga gtgccaaggg ctggctgctc gcattgatgg ttgcactgat
     7681 cgccggcggc gccgcgcccg cggcagcgcg gccggtcgac ctcgctttgg tgctcgcggt
     7741 cgacgtgtcg gggagcgtcg acgccgagcg ctatgagctg cagcgccacg gctatgccga
     7801 ggccttcgcc agcaacgaag tgatcgccgc catcgccgcc ggcgagaacc acgcgatcgt
     7861 tgtgactttg gtggagtggt cgggcgccgc gcatcagcag cagatgatcg cctggacgct
     7921 catccacgac gcggcctccg ccgaagcctt cggcagcgcc atcgccgaga cgccacgcgc
     7981 cttctccgac tggacctcga tcagcgcggc gctcgactac gcggtggctt tgttcgccga
     8041 tgccgaggtc gaggccgcgc ggcgcgtcat cgacatctcg ggcgacggcg tcaacaacaa
     8101 cgggcgctcg atcatcgagg cgcgcgatgc cgccatcgcc gccggcatca ccgtcaacgg
     8161 cctgcccatt ctctcggagt atccgacgct cgacggctac taccgcgaga acgtgatcgg
     8221 cggccccggc gctttcgcag tcgcggtaaa tgatttccaa ggattcggcg ccgccatcct
     8281 gagcaagctt gtgcgcgaga tcgccgggac ccaagggcca tccgtggtta ggttcgcagg
     8341 ccgtgcgcgc ggtagaaacc caggctagga tgtgcctcgg ggagagaccg tcatgctcgg
     8401 gtccgcgtcg tcagggaaga agcgcctgcc ccgcttggcc ggcatcgctg cagcgttgct
     8461 gctggcggcg ggggttgcct gcgccgacga ggcgctgccg ccgatctggc agatggtcga
     8521 gaagaggccg ccgggcccgc tgccgggcga gccggtcctc ggcaaggccg acacgccgct
     8581 caccatcatc gaatatgcct cgctgacctg cccgcattgc gcccgcttcg cgacgcagac
     8641 gctgccgaag ctgaagcagg agtggatcga caccggcaag gccaaactgg tgttccgcga
     8701 ttttcccttg gacggcctcg cgctccgcgc cgccgcgctc acgcattgcg tgccgcccga
     8761 gcgctatctc gaattcgtcg acacgctctt caaggagcag ccgagttgga tcgtcctcgg
     8821 cgacaaagcg atcgcgcgca tcgcgagcct gggcggcctc agcgaagcgc aagccgaggc
     8881 ctgccttgcc gacaccaagc gcgtggaatc gatttcggct gcgcgcgccg cggccgagaa
     8941 ggagtggggc gtcgatgcca cgccgacatt cttcatcgac ggcatgaaga tctcgggcgc
     9001 ctacccctat gtggatctcg agagcaagga tcgggatttc aagagcgccc tcgccaccgc
     9061 tcttgcgcaa aagcagccat ccgacgcgca ttagtggtcg cgcggagcgt cctggccgag
     9121 gtgcgcgagc ttctccttga ttgccccgcc gatatagatc tggggctcag agggcgtcag
     9181 cttctcttcc tgctcgcggg aggcgatgag gcgttgcgcc tcgtcgaacg ccgcgatgaa
     9241 ggaagagcca tggcgtagcg cctgattgaa ataggcgtcg ccgaaataag tgaaatcgtt
     9301 ctcgttgctg cagccgaagg agctgcggtc gcgtgccgcg gcggtgatga tcagcgattg
     9361 ccggtccttc agcgcatcga cgaagcttcc ggagtagcag gcggagatga cgatgacgcg
     9421 ccagcggatg ccggccgcgt cgagcatgtg gcgcaggtcc tgcggggcga tatcttcgag
     9481 ctggaggggc gggaatgaga ccgcgagttc gttctcggcg ccatgcgagg tcagaaagag
     9541 gaacagcacg tcgttgtcga ggttcatttt cgcggcgatg acgtcgagtg ccaggcggag
     9601 gttgccggcg ctggccaccg gcagttcttt taccgtgtcg aggttgttga cgaggagcaa
     9661 cgaacggccg cgcgtgccga agcgctcgtc gaagagcgtg cgcaccgtct ccacttcctt
     9721 cataaacacg tcctgtccgg catacccggc gaagcccagg aaatagaggt cgggcgcgcc
     9781 gcgtcggtgg gacgcgaccg cttcgatttc ctgcgcgaga agccagggct ggtcatgaag
     9841 gatcgtgtcc gacgtgagga cgagatgctc ctgctcctcc gcccgggctt gggccgtgcc
     9901 gagcaagagc gcggcaagca acacgcggcc cagcggcgca acgatcatcg aaagccccca
     9961 gcccttcacg gcgtcaccat gagaatctcg gcgccctcga cgcgaagccc gatgcgctcg
    10021 aggctcttcc ccgcgcaagg accgtcgatg cagaagccgt cggcgatgcg gaagcgcgcg
    10081 ccatgggtgc tgcatagcag atgccgcccg tcgcgatcga ggaaccggtc gggcaggaag
    10141 tcgagcggcg tgccgagatg cgggcaggaa tt
//
LOCUS       DASYNS010000457         5236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1679732, whole genome shotgun sequence.
ACCESSION   DASYNS010000457 DASYNS010000000
VERSION     DASYNS010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679732"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VGP50_14280"
     CDS             <1..358
                     /locus_tag="VGP50_14280"
                     /inference="COORDINATES: protein motif:HMM:NF023950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3734 domain-containing protein"
                     /protein_id="HEV8028598.1"
                     /translation="REQDIQFASREDSHIARQEQLHQLRHVVRELAKRIPKEQRNDPE
                     VRELAAFGCTTTMHVVRLFAPRLENEDFTKALDFTPASIKVRWEAGYAHAKQMLQEKP
                     WHNELGPLQSAVLHDV"
     gene            complement(395..2260)
                     /gene="cobT"
                     /locus_tag="VGP50_14285"
     CDS             complement(395..2260)
                     /gene="cobT"
                     /locus_tag="VGP50_14285"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEV8028599.1"
                     /translation="MAENETPLDVFKRATAAAVRAIAEREEVTVTYGTEPAAAAGTRV
                     RLPLPSRDLSAADVAPVRGAADAAALRLRYHDQALHAKRLPTGETARAIFEGVEQARV
                     EALGARRMTGVAANLAAMLDERYRRQGYEHLAERNDVTLAEAVRLLAREALTGALPPP
                     AARRVVELWRPFLEGRVGRDLHELSRHVGNQEAYAKTARQLIRDLDLDLGETEQESEA
                     EDAEGEGDEADQGENQNDGGEGATAGAQGALEGGAPEHEQDDGQPAGVDEIEGELRPG
                     AGDDDPGRPGRPGQLPRHGINDDPTAYRPFTTECDETVDASALCDPDELTRLRGLLDQ
                     QLAHLQSVISRLANRLQRRLMAKQTRAWEFDLEDGMLDAARLSRVITNPVYPLSYKRE
                     KETDFRDTVVTLLIDNSGSMRGRPITVAAMSADILARTLERCGVKVEILGFTTRAWKG
                     GQSRERWIAAGKPAHPGRLNDLRHIIYKAADAPWRRARRCLGLMLREGILKENIDGEA
                     LLWAHSRLVARTEQRRILMVISDGAPVDDSTLSVNPGNYLERHLREVIAEIEQRSPVE
                     LVAIGIGHDVTRYYRRAVTIVDAEQLGGAMLDKLAELFDEDVKQPTLPMKRARAR"
     gene            complement(2281..3276)
                     /gene="cobS"
                     /locus_tag="VGP50_14290"
     CDS             complement(2281..3276)
                     /gene="cobS"
                     /locus_tag="VGP50_14290"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HEV8028600.1"
                     /translation="MASPQGLNETPIDQPDITVSVRQTFGLDSDMHVPAFSHPTDHVP
                     DLDEAYRFDHDTTLAILAGFAYNRRVMIQGYHGTGKSTHIEQTAARLNWPCIRVNLDS
                     HISRIDLVGKDAIVLRDGKQVTEYREGLLPWALQHPTALVFDEYDAGRPDVMFVIQRV
                     LEVEGKLTLLDQNRVIRPHPAFRLFSTTNTVGLGDTTGLYHGTQQINQGQMDRWNIVA
                     TLNYLSHDVEVEIVLAKAPSWDTEDGRRAINAMVALAELTRSGFINGDISTVMSPRTV
                     ITWAENARIFNDVAFAFRLTFLNKCDEVERSTVAEYYQRCFGSELKETGVRAQLV"
     gene            complement(3330..3899)
                     /locus_tag="VGP50_14295"
     CDS             complement(3330..3899)
                     /locus_tag="VGP50_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEV8028601.1"
                     /translation="MPRTSIESSAGPSAAATAARLCDHPGCSAGGDYRAPRSRDRLDV
                     FFWFCLDHVRAYNAQWNYYAGMSAAEIEAEIRNDTVWQRPTWPMGGRYGLRFDAHVRD
                     FGLFGFDDEDPELRERPKKRPLTEQEQALAVFELSLPLTLGGLKSRYKALVKLHHPDA
                     HGGDRAAEERLKVINQAYSTLKSSYFPSG"
     gene            4013..4309
                     /locus_tag="VGP50_14300"
     CDS             4013..4309
                     /locus_tag="VGP50_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEV8028602.1"
                     /translation="MRVADRIRSKLTTALQPTRLAIRDDSQRHAGHAGARPEGETHFH
                     VEIVSPLFAGMSRVARQRRVYQALAAEMAGPVHALGLVTLTPAEDGNSKKDGNS"
     gene            4401..4742
                     /locus_tag="VGP50_14305"
     CDS             4401..4742
                     /locus_tag="VGP50_14305"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEV8028603.1"
                     /translation="MQRDTTQPHLPLSAGAASAVKSVSTLVNRNVTVGGHRTSIRLEP
                     AMWEALHHICRREGKALNELVTEIHRHRAESSLTAAIRVYLLRYFSAAATDEGHRLAG
                     HGLPIGSQLPV"
     gene            complement(4727..>5236)
                     /locus_tag="VGP50_14310"
     CDS             complement(4727..>5236)
                     /locus_tag="VGP50_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEV8028604.1"
                     /translation="RREDGLWDIEGHITDVKTYAFGNHFRGEIPAGTPVHDMWLRLTV
                     DDRLTIHEVEAVTEASPYKVCPNVAPNFARLKGLRIKPGFQAEVRGRLGGTEGCTHLV
                     ELLVPVATTAYQTVFPYRERQRREQRKAEGKPRERPSRRPPLLDTCHAFASDGEVVKR
                     NWPDFYTGS"
BASE COUNT          849 a         1699 c         1784 g          904 t
ORIGIN      
        1 ccgcgagcag gatatccagt tcgcgagccg cgaggacagc cacatcgccc gccaggagca
       61 gctccaccag ctccgccacg tcgtgcgcga gctggccaag cgcattccga aggagcaaag
      121 gaacgatccc gaggtgagag agcttgcggc cttcggctgc accacgacga tgcatgtcgt
      181 gcgtctgttc gcgccgcgcc tcgagaacga agacttcacc aaggccctcg atttcacgcc
      241 cgccagcatc aaggtgcgat gggaagcggg ctacgcgcac gcaaaacaga tgctccaaga
      301 gaagccgtgg cacaacgagc tcgggccgct gcaatcggcg gtgctccacg acgtctgatc
      361 acgggacggc cggcggccct ggactctcgt ggccctaccg cgcgcgcgcc cgcttcatgg
      421 gcagcgtggg ctgcttgacg tcctcgtcga agagctcggc gagcttgtcg agcatggcgc
      481 cgccgagctg ctcggcgtcg acgatggtga cggcgcggcg gtaatagcgt gtgacgtcgt
      541 ggccgatgcc gatggccacc agctcgaccg gcgatctctg ctcgatctcg gcgatgacct
      601 cgcgcagatg gcgctcgagg tagttgccgg gattgaccga cagagtcgaa tcatcgaccg
      661 gcgcgccgtc ggagatgacc atcaggatgc gccgctgctc ggtccgggcg acgagccggc
      721 tatgcgccca gagcagcgcc tcgccatcga tgttctcctt gaggatgccc tcgcgcagca
      781 tcagcccgag gcagcgccgc gcgcgccgcc acggcgcatc cgccgccttg tagatgatgt
      841 ggcgcagatc gttgagccgg ccgggatgag cgggcttgcc cgcggcgatc cagcgctcgc
      901 gcgactgacc gcctttccag gcgcgggtgg tgaagcccag gatctcgacc ttgacgccac
      961 agcgctcgag cgttcgcgcg aggatgtcgg cgctcatggc cgccacggtg atcggccgtc
     1021 cccgcatcga gccggaattg tcgatgagga gagtcaccac cgtgtcgcgg aaatcggtct
     1081 ccttctcgcg cttgtaggac agcggataga ccggattggt gatgacgcgc gaaagacgcg
     1141 cggcatcgag catgccgtcc tcgagatcga attcccaggc gcgcgtctgc ttggccatga
     1201 ggcgccgctg cagccggttg gcgaggcgcg agatcacgct ctgcagatgc gcgagctgct
     1261 gatccaggag cccgcgcagc cgggtcagct cgtcgggatc gcagagcgcg ctcgcatcga
     1321 cggtctcgtc gcattccgtg gtgaaggggc gataggcggt cggatcgtcg ttgatgccgt
     1381 gccgcggcag ctggcccgga cggccggggc ggccaggatc gtcgtcgccg gcgcccggcc
     1441 tgagctcgcc ttcgatctca tcgacgcctg ccggctggcc gtcatcctgc tcgtgctcgg
     1501 gcgcgccgcc ttcgagcgcg ccttgggcgc cggccgtcgc accctcgccg ccatcgttct
     1561 gattctcgcc ctgatcggct tcgtcgccct cgccttcggc gtcctcggct tcgctttcct
     1621 gctcggtctc gccgagatcg agatcgagat cacggatgag ctgccgcgcc gtcttggcat
     1681 aagcttcctg gttgccgaca tggcgactca gctcatggag gtcgcggccg acgcggccct
     1741 cgaggaaagg acgccacagc tcgaccacgc gccgcgccgc gggcggcggc aaggcgccgg
     1801 tgagcgcctc gcgggcgagg agccgcaccg cctcggccag cgtcacgtcg ttccgctcgg
     1861 cgagatgctc atagccctgg cggcgatagc gttcgtcgag catagccgcg aggttggcgg
     1921 caacgccggt catgcggcgg gcgcccagcg cctcgacccg tgcctgctcg acgccttcga
     1981 agatggcgcg ggcggtctcc ccggtgggca gccgcttggc gtggagcgcc tgatcgtgat
     2041 aacgcagccg gagcgccgcg gcgtcggcgg cgccgcgcac cggtgccaca tcggcggcgc
     2101 tgaggtcgcg cgagggcagc ggcaagcgca cgcgcgtgcc ggcggccgcc gccggctcgg
     2161 tgccataggt caccgtcact tcctcgcgct cggcaatggc gcgaacggca gcggcagtcg
     2221 cccgcttgaa gacgtcgaga ggagtctcgt tctcggccat gaaacgtccg gggccggttt
     2281 ctagaccagc tgcgcccgaa cccccgtctc cttgagctcg gagccaaagc agcgctggta
     2341 gtattcggcc accgtcgagc gctccacctc gtcgcatttg ttgaggaagg tgaggcggaa
     2401 ggcgaaagcg acatcgttga agatgcgcgc gttctcggcc caggtgatca ccgtgcgcgg
     2461 cgacatcacc gtcgagatgt cgccgttgat gaagccagag cgcgtgagct cggcgagcgc
     2521 caccatggcg ttgatggcgc ggcgcccgtc ctccgtgtcc cagctcggcg ccttggcgag
     2581 cacgatttcg acctcgacgt catgcgacag atagttgagc gtcgcaacga tgttccagcg
     2641 gtccatctgc ccctggttga tctgctgcgt gccgtgatag aggcccgtgg tgtcgccgag
     2701 gcccaccgtg ttggtggtcg agaagagacg aaacgccgga tgcgggcgga tgacgcggtt
     2761 ctggtcgagc agcgtcagct tgccctcgac ctcgagcacg cgctggatga cgaacatcac
     2821 atcgggacgg ccggcatcgt attcgtcgaa gaccagcgcg gtcggatgct gcagcgccca
     2881 agggaggagg ccttcgcgat attccgtcac ctgcttgccg tcgcgcaaca cgatcgcgtc
     2941 tttgccgacg agatcgatgc ggctgatatg gctgtcgaga ttgacgcgga tgcagggcca
     3001 gttgagccgt gccgcggtct gctcgatatg cgtcgacttg ccggtgccgt gatagccctg
     3061 gatcatcacg cggcggttgt aggcaaagcc cgcaaggatg gccaaagtcg tgtcgtggtc
     3121 gaagcgatag gcctcgtcga ggtcaggaac gtggtcggtc gggtgcgaga aggccggcac
     3181 gtgcatgtcg ctgtcgaggc cgaaggtctg gcgcaccgaa acggtaatgt ccggctggtc
     3241 gatgggtgtt tcgtttagtc cttggggcga agccattggg ccctgtcgaa gctggtgaag
     3301 gaaagacttc ggatgcgcgg cgaccgcggt catcccgacg ggaaatagct gctctttagg
     3361 gtcgagtagg cctggttgat cactttgagt cgctcctcgg ccgcgcggtc gccgccatgg
     3421 gcatcggggt gatgaagctt caccagagcc ttgtagcgcg acttgagccc gcccagcgtc
     3481 aagggcagcg agagttcgaa gaccgccaga gcctgctcct gctcggtgag cggccgcttc
     3541 ttcgggcgct cgcggagctc cggatcttcg tcgtcgaagc cgaagaggcc gaaatcgcgc
     3601 acgtgggcgt cgaagcgaag gccgtagcgg ccgcccatgg gccaggtcgg ccgctgccag
     3661 accgtatcgt tgcggatctc ggcctcgatc tccgcggcgc tcattccggc gtagtagttc
     3721 cactgcgcgt tataggcgcg cacatgatcg agacagaacc agaagaacac gtcgagccgg
     3781 tcgcgcgagc ggggcgcccg gtagtcgcca ccggcgctgc agccgggatg atcgcagagc
     3841 cgcgccgccg tggccgccgc ggacggtccg gcactgcttt cgatgctcgt acgtggcatg
     3901 cgcatgagta tggtgagtat ggggttaacc aatagccctg acaagcgctc gcggcgcaac
     3961 attgggtcac cgccttgaca gggctggggc ggctttgcca gggtcgcgcc gcatgcgcgt
     4021 cgccgatcgt atacggtcga agcttaccac ggcgttgcag ccgacgcggc tggccatccg
     4081 ggatgattcc cagcgccacg ccgggcatgc cggcgcccgg cccgaaggcg aaacccactt
     4141 ccatgtggag atcgtgtcgc cgctctttgc gggaatgagc cgcgtcgcgc gccagcgccg
     4201 ggtctaccag gcgctggccg ccgaaatggc ggggccggtc cacgctctgg ggctcgtgac
     4261 cttgacgccc gcggaggacg gcaattccaa gaaagacggc aattcttaag acaaaagcgc
     4321 aatttgcaac ttaaaagtgt tggcagaaat tttgcactcg aaagcgttgc atttggctcc
     4381 agttgcgtta gattgtatgc atgcaacgcg atactactca accccatctg cctctgtccg
     4441 cgggcgcggc cagcgcagtc aaatcggtct cgaccctcgt caatcgcaat gtgacggtcg
     4501 gcggccaccg gaccagcatc agacttgagc cggcgatgtg ggaggcgctt caccacatct
     4561 gccgacggga gggtaaggcc ctcaacgagc tggtcacgga gatccaccgt catcgggcgg
     4621 aatcgagcct gacggcggcg atccgcgttt atctgctgcg ctacttctct gctgcagcga
     4681 ccgatgaagg ccatcgcctt gccggccacg gcctgcccat cggcagtcag ctgccggtat
     4741 agaagtccgg ccagttgcgc ttgacgactt cgccatcgct ggcgaaggcg tggcaggtgt
     4801 cgaggagcgg gggccgccgg ctcggccttt cccgcggctt gccttccgct ttccgctgtt
     4861 cccgacgctg gcgctcgcga tagggaaaga ccgtctgata cgcggtggtc gccaccggca
     4921 ccagtagctc gacgagatgc gtgcatccct cggtgccgcc cagccggccg cgcacctccg
     4981 cctggaaacc cggcttgatg cggagcccct tgaggcgcgc gaaattcggc gcaacgttcg
     5041 ggcagacctt atagggactc gcttccgtca cggcttcgac ctcgtggatc gtcagccggt
     5101 cgtccaccgt cagcctcagc cacatgtcat gcacgggcgt gccggccgga atctcgccac
     5161 ggaaatggtt gccgaaggcg taagtcttga cgtccgtgat gtggccttcg atgtcccaca
     5221 acccatcctc gcggcg
//
LOCUS       DASYNS010000458        21968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1052551, whole genome shotgun sequence.
ACCESSION   DASYNS010000458 DASYNS010000000
VERSION     DASYNS010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 21968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21968
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052551"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VGP50_14315"
     CDS             complement(<1..284)
                     /locus_tag="VGP50_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028605.1"
                     /translation="MSNVTTPAEPAVAAGSPERFGYEWHTYAEMRAEYEEQFRRWTVH
                     LSPEDWRGKLFLDVGCGMGRNSYWPMTYGAAGGVAIDVDERSLASARRTLS"
     gene            complement(301..2178)
                     /gene="asnB"
                     /locus_tag="VGP50_14320"
     CDS             complement(301..2178)
                     /gene="asnB"
                     /locus_tag="VGP50_14320"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV8028606.1"
                     /translation="MCGIAGFSGLGDRADLAAMTESLAHRGPDGEGQFVDDEAKVYLG
                     HRRLAIIDQDGGRQPMWSDDGSIGVVFNGEIYNHRELRARLEARGHVFRSSHSDTEVL
                     VHGYREWGESLPSQLNGMFAFAVYDRRRKRLFLARDRFGEKPLYYYQRPLLFAFASEI
                     TALRKHRAFSEKLSPRALQKFFAFGYVPAPDAIYEGVAKLPGGHCLIFDLTSGESSVF
                     RYWRFRIEPDDSLTDADEERLAEELRHLLTQAVGRRLMSDVPLGVFLSGGIDSAAVLA
                     AAARHLPPARLRAFTVGFAEPSFDESAAARQTADFIGSRSAVEILDLETARRLIPDVL
                     KRLDEPLADPSIIPTYLLSRFTRQQVTVALSGDGGDELFAGYDPFRALAPARYYHRLM
                     ADPLHRGLRRLADLLPLSTRNMSWDFKLRRALTGLSYDASLWNPVWTAPVEPDALQDL
                     FDEPLHLDDIYGDAMALWEEDSRKSVIDKTLEYYTNFYLPDDILAKVDRASMMNSLES
                     RAVFLDNDLVAFCQRLPARFKYRGGRRKYLLKRAVEPWLPKAIIERPKKGFGIPIAAW
                     LRELAPPQPAAMIEGVNHAFVDSAWRDHRAGKRDHRLLLWCWLSLQALVTGNDRYRAA
                     A"
     gene            complement(2184..3221)
                     /locus_tag="VGP50_14325"
     CDS             complement(2184..3221)
                     /locus_tag="VGP50_14325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV8028607.1"
                     /translation="MMAQPERSKHFAATVGERQRLVADAFSAPELSVIIPLFNEEENV
                     ERLFSTLLPTLRALSLRFEIIVVNDGSSDGSLAALKAVAAHAPELRIVDFRRNYGQTA
                     AMMAGIDHAAGRIIVSMDADLQNDPADIPLLLAKIEEGYDVASGWRQDRKDAPLRRNL
                     VSRVANRLISRVSGVRLNDYGCTLKAYRADVVRGIRLYGEMHRFIPIYASWMGGKVVE
                     VPVRHHARHAGKSKYGLERVLKVVLDLIVVKFLDAYFVKPIYVFGGFGIFSLFLSSVF
                     FGAMMYLKLVRGLSMILTPLPLLTAMAFLVGILSILMGLVAEMLVRTYFESQGRSPYL
                     VRELVNFEPEG"
     gene            complement(3532..4983)
                     /gene="rpoN"
                     /locus_tag="VGP50_14330"
     CDS             complement(3532..4983)
                     /gene="rpoN"
                     /locus_tag="VGP50_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEV8028608.1"
                     /translation="MAFSQRLDLRQTQALVMTPQLQQAIKLLQLSNLELTAFIDSELE
                     QNPLLERDDAPAEADGATPAAAEESGTAEDAEEVALWRETAGADGEGNLDLAGDPAAW
                     NGRAPRAAAATDLPGLDQTLSRPPTLRDHLLAQLSVDIGDPVERLIGAHLVEMVDEAG
                     YLQGDLAEVAERLGCEVAQVDTTLSLLQRFDPAGVFARDLAECLALQLKERDRFDPAM
                     QALVANLPLLASRNAPALLRLCNVDAEDLAEMIAELKALNPKPGLAFDPASAELVVPD
                     VLVRPHAGGWVVELNSDTLPRVLVNNHYYAAVSGALRSKTEKDYLAERYQQANWLLKA
                     LHQRATTILKVSTEIVRQQEGFFRQGVRHLRPLVLRDIAEAIGMHESTVSRVTSSKYM
                     ATPRGIFELKYFFTSAIAATSGGEALSAEAVRFRIKALIDAENEDVLSDDRIVEILLK
                     EGIDIARRTVAKYREAMRIPSSVQRRRERLLPL"
     gene            5185..6429
                     /locus_tag="VGP50_14335"
     CDS             5185..6429
                     /locus_tag="VGP50_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028609.1"
                     /translation="MTVESIAVGGAAGNTAASPIGVAKLSKMQFDGTDLTPLWRELIG
                     KYIHEPDDAAALLDLAVIEQLFGDREAGCARQADALKLCRIYRSPFPGAAPSLRLLAF
                     AVPGDIGANTPLEFLLEGSDVELHTLYLVPGEPIPAAVPQHDIAFVAIGEADDHRIAL
                     DTIETLMASWPRPVLNRPDRIALLSRERLHGLLATAPALVIPATTRLDRASLVKLGRG
                     DVELHALMTDASFPLILRPVGSHAGHGLQKLDDAPALAAYLAERPEALFYASRFIDYR
                     SPDGLFRKYRIVFIAGRPYACHMATTDQWMIYYLNANMKESAAKRVEEEQFMARFDTD
                     FARRHAAALAAVAERVGLDYFGIDCAETRDGRLLIFEADIAMIVHAMDDPQIFPYKGP
                     QMRKVFDAFRAMLRERSAKALS"
     gene            6426..7886
                     /locus_tag="VGP50_14340"
     CDS             6426..7886
                     /locus_tag="VGP50_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019091111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV8028610.1"
                     /translation="MSDETLDPDDWEGVRQVFHEAVDRAIDHLRDIRRKPVWTATPEA
                     TKKKLRAKLPHAAEPLDHLLSSFVQDILPYGTGNIHPRFFGWVHGSGNVAGVLGEMLA
                     SFMNCNVGGRDHVAVYVERQVIDWCKEIFAYPATSSGLLTSGTSMATVIALAVARFAK
                     AGASVRTDGVAAAARPLTGYASSEAHGSVGKAFDLLGLGQGALRAIPVDADFRMRLAD
                     LERQVAQDRAAGRMPAIVVASCGTVNTGAIDDVAAIAAFCRAQGLWLHIDGAFGGAAV
                     LLPEFRAKLAAIAEADSIAFDFHKWLHVPYDAGCVLVKDAAAHRAAFAMRPDYLAAAH
                     RGLAGGEPWFCDYGPELSRGFRALKVWFTIKAHGIDRLSAAMARNCRQARLLAETVTA
                     SADLELLAPVELNIVCFRFRAAGVADAALSRLNEEIVADLQEEGIAAPSTARIGGRTA
                     IRVCLTNHRTGDEDLALLLDAIRRLGNERLARAETR"
     gene            7883..9511
                     /locus_tag="VGP50_14345"
     CDS             7883..9511
                     /locus_tag="VGP50_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028611.1"
                     /translation="MTAPPFRQLGEIFAPLDHILASGGDARLHIDPITGLNGYGCRPY
                     PRPDASTFASSTATSISERAYACAEAEHHDLIRAGLRQGFETAVDDRFEQLRDELRTV
                     LDLADSGIEIVFSPSGTDSTLQALLVARAMLGTPLTSVIAAADETGSGVGDAASGRHF
                     SALTSQAVIVKRGDPIQGLAEGVRRIAIPLRDERGHARPPGVMDEQVKRAVAAAAAAG
                     ERILLHAMDHSKLAAQCPSLDCLRAVADAAPGAVQIVIDACQMRISRTRLKRHLAEGH
                     MVLITGSKFFTGPPFSGALLVPGALSQRMAALDVVPAGLADYSSRSDWPRAWQGLRAA
                     LPARVALGQLLRWIAALGEMRAYFAVPPSFRSFALARFATVVPALIAATPTRELLVDR
                     SCGDGHATDDEEMASPTIFPFRLRRAGRLLSLEEARLVYRALNADVTAMLPAAATAAD
                     RQLAAQLCHVGQPVGLADPPRGTSGALRISAGARFVSESWSPAGESVAMANLDGEFAQ
                     IRAILAKIDLLLRHFEALGRWDARRTERKVARAG"
     gene            complement(9635..10429)
                     /gene="lptB"
                     /locus_tag="VGP50_14350"
     CDS             complement(9635..10429)
                     /gene="lptB"
                     /locus_tag="VGP50_14350"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEV8028612.1"
                     /translation="MTPESADAPRSAGRPGAVPRLVANNRGLVATNLGKSFKKRPVLR
                     DVNVSVQRGEAVGLLGPNGAGKTTCFYIITGLIAPDVGTIALDSQDITELPMYRRARL
                     GIGYLPQEASIFRGLTVEQNIRAVLEVAEPVPAVRETMIDELLAEFSITHLRRAPALA
                     LSGGERRRVEIARALATQPHFILLDEPLAGIDPIAVSDIRDLVAHLKDRGIGVLITDH
                     NVRETLEIVDRAYILHEGRVLMEGSPAEIVSHADVRRVYLGEKFSL"
     gene            complement(10426..11424)
                     /locus_tag="VGP50_14355"
     CDS             complement(10426..11424)
                     /locus_tag="VGP50_14355"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEV8028613.1"
                     /translation="MTSRFLAILLAFAPGLLLAPVTQAQNFGLGLNSQDNGKPITIEA
                     EQDIEWQQNNRVYIARGHAKATRGQATVFGDTLYAYYRPVANATNGNSAPPPPPASPA
                     GGGKPGDPTSGSSTEIYRLEADGNVRIATETQTVYCDHAIYDVDQTLLVATGKHLKLE
                     TPRDTVTARDSLEWYDSKQRAVARGDAVAVQEGGKALRGDVLTADVTKGPDGSSHISR
                     IDAQGNVLVSSQGQIGRGDTGVYNVDTGIATLAGNVTLTRGDNELRGQYGVVDLNSNV
                     SRLLSAPPGDQVSEGPRPQVHGLLVPRPKPEAAGAKPDAVAAKSDGPASKPAAPPQ"
     gene            complement(11421..12086)
                     /gene="lptC"
                     /locus_tag="VGP50_14360"
     CDS             complement(11421..12086)
                     /gene="lptC"
                     /locus_tag="VGP50_14360"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEV8028614.1"
                     /translation="MTAPPDTFLGAGEVGRPRRRAVFSRRYSRFVSLAKRIFPVIAVS
                     LIVLVAVWPRIEEAIEHVHFALPRIDLREAHDLRMVHAHYSGIDRQQRPYVITAEIAR
                     QNPGAADIVALEGPKGDLTTLTGGWFEVTSRTGLYQPQAQLLDLFGDVQLFQDKGNEF
                     HSESAHVDMANGTAEGHEPIEGQGPFGHATGQGFRILDHGDVVIFTGRSRLDLAPHEK
                     AGQ"
     gene            complement(12083..13054)
                     /locus_tag="VGP50_14365"
     CDS             complement(12083..13054)
                     /locus_tag="VGP50_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEV8028615.1"
                     /translation="MLSNAPDIDAARRVLRTEIAGLEALAEELNGTFAAAVEKLAQVR
                     GRVTVTGMGKSGHVARKIAATFSSTGTAAQFVHPGEASHGDLGMIAGGDAVLALSNSG
                     DTAELGDIVAYTRRFRIPLVAMTRRGRSTLAEAADVTLLLPATEEACPMGLAPTTSTT
                     MMMALGDALAVALLERKGFSASDFQVLHPGGQIGRQLLRVADVMRTGDAVPLVPSGTP
                     MAEAILRMSEKGLGCVGITNAKGCLVGIITDGDLRRHMADDLLRSTVDAVMTKGPKTI
                     RPQALAAEGLGRMHAPPGAIKNLFVVDEAERPLGILDLHDCLREGVA"
     gene            complement(13182..13793)
                     /locus_tag="VGP50_14370"
     CDS             complement(13182..13793)
                     /locus_tag="VGP50_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEV8028616.1"
                     /translation="MSIHLHHGDLPPGLDFGRSVAIDTETMGLDPHRDRFCLAQLSAG
                     DGVCHLVQFAAGVYDAPNLKVLLADPEVLKIFHFARFDIAVMERHLGVMPRPLYCTKI
                     ASRLVRTFTDRHGLKDLCRDLLGVELSKQQQSSDWGAAELTDEQLRYAASDVLHLHAL
                     KAKLDQMLAREGRQAVAQACYEFLPTRALLDLGGWAHEDIFAH"
     gene            complement(13817..13901)
                     /locus_tag="VGP50_14375"
     tRNA            complement(13817..13901)
                     /locus_tag="VGP50_14375"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13865..13867),aa:Leu,seq:cag)
     gene            14038..15012
                     /locus_tag="VGP50_14380"
     CDS             14038..15012
                     /locus_tag="VGP50_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEV8028617.1"
                     /translation="MRYQRVLIVGGTGFIGRYVVKRLAARGTVIAVVSRNADAAGFLR
                     PLGDVGQIALINAGLGNERRLRALLEEGMDAVICCAGVLYSRGRQTFAEVHHEGPARL
                     ARLATASGVKRFVHVSALGADAASPSVYGRSKAAGEAAVRAAFPEAVIFRPSVVFGPE
                     DDFFNRFASLARYSPVLPLIGGGLTRFQPVYVGDVADAVAAALDRHDSAGRIFELGGP
                     LVYSFKELMELLLREIDRRRALVSLPVPLAQLMAFFLEWLPSPPLTRDQVRLLQKDNV
                     VEPGVPDLGSLGVTPTSLELVLPTYLDRFRRGGRFGLARTAPAGPLNE"
     gene            15230..16669
                     /locus_tag="VGP50_14385"
     CDS             15230..16669
                     /locus_tag="VGP50_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8028618.1"
                     /translation="MKPGLSQFVGLEKAMPEKRPAEQRRHDFKEIHGEFDPARARQQA
                     SRCSQCGVPYCQVHCPLGNNIPDWLMLTAAGRLEEAYEVAAATNNMPEVCGRICPQDR
                     LCEGNCVIEKGFSSVTIGSVEKYITETAFAKGWVKPPLPRHELGQSVGIIGAGPAGLA
                     AAEQLRRKGYAVHVYDRYDRVGGLLVYGIPNFKLEKRVVARRHELLVLGGIIFHLDFE
                     VGRDADLATLRQRHDAVLIATGVYQARAMACPGIGLGNVVAALDYLTASNRKGLGDAV
                     PAFDSGALNAVEKDVVVVGGGDTAMDCVRTAVRQGAKSVRCLYRRDRKNMPGSQREVR
                     HAEEEGVEFVWLAAPEALLGESEVREVRAHRMHLGVADSSGRQVPQPLPGSHFMLPAD
                     LAILALGFDPEEVPRLFGAPEIALRPDGTIKIDFRSMMTSLDGVFAAGDIVRGASLVV
                     WAIRDGRDAAEQIHRYVQQKAAAVVAAAE"
     gene            16675..17280
                     /locus_tag="VGP50_14390"
     CDS             16675..17280
                     /locus_tag="VGP50_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEV8028619.1"
                     /translation="MLRLHDNASSGNGYKVRLLLTLLGRPFGRIEYDVDRGATRTAEF
                     LSKNLNGRIPVLELEDGRFLAESDAILVYLAEGTRFLPQDRYERALALQWLFFEQYSH
                     EPNIATSRYWITHGLLNDERRALLDGKRRQGHAALAVMERHLEGRRYFVGERYSVADI
                     ALYAYTHVADEGGFDLAGYPAVRKWLDRVASEPGHIPITKV"
     gene            17286..21842
                     /gene="gltB"
                     /locus_tag="VGP50_14395"
     CDS             17286..21842
                     /gene="gltB"
                     /locus_tag="VGP50_14395"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEV8028620.1"
                     /translation="MTEHQPPSPSQWADDYRRNAAHLEAAGLYDPAEEHDACGVGFVA
                     SLDGKARRAVVAAAIDALRAVWHRGAVDADGKTGDGAGIHVEIPQEFFRAHVRHTGLE
                     PGPGHLAVGMVFLPRTDLDGQERCRSIVEAEILNAGYAIYGWRQVPVNIDIIGEKANA
                     TRPEIEQIMIANARGAEVERFELDLYVIRRRIEKRVIADQVKDFYICSLSCRSIVYKG
                     MFLAEQLTAFYPDLADERFVSRFAIYHQRYSTNTFPTWPLAQPFRILAHNGEINTVRG
                     NINWMKSHETRMASDVFGDAIEDLKPVIQVGGSDSAALDAVFELLARSGRVLPMVKTL
                     MVPEAASDARLMPETHRDMYSYCNSVMEPWDGPAALAMTDGRWVLGGLDRNGLRPMRY
                     AVTSDGLIIAGSEAGMVRLDETTIIEKGRLGPGQMIGVDLASGKLHRDREIKDRLAAG
                     RPFSAWVKGITPLDSLIRPSHGEKAELGRDELRRRQVAFGWSLEDLELILQPMAEDGK
                     EAVGSMGDDTPLAVLSDHYRGLHHFFRQNFSQVTNPPIDSLRERRVMTLRTRLGNLGN
                     ILDEDASQCRLLQLDSPVLSNAEFAAMRAYMGETAAEIDCCFEAQGGSAALKAAIRRV
                     QQEAEDAVRGGCTHVILSDSGMSAKRAAIPMILAAGAVHSHLVRQQLRTFTSLNVRSG
                     ECLDVHYFAVLIGVGATTVNAYLAQETITDRHRRGLFAPGSSVEAAVERYKQAINDGL
                     LKVMSKMGIAVISSYRGAYNFEAVGLSRTLVAEYFPGMTSRISGIGLAGLEIKTLETH
                     RRAYAEDVIALPVGGFYRYRRGGELHAFEGTLIHTLQTAVESDSYGAYRRYSEGMRKL
                     PPIHLRDLLDFRPKRPAVSVDEVESITEVRKRLVAPGISLGALGPEAHETLSIAMNRI
                     GARSDSGEGGEDPARYRPRANGDNASSAIKQIASGRFGVTAEYLNNCREIEIKIAQGA
                     KPGEGGQLPGFKVTEMLARLRHSTPGVSLVSPPPHHDIYSIEDLAQLIYDLKQINPEA
                     KVCVKLVARSGIGTIAAGVAKAKADVILISGHSGGTGASPLSSIKHAGVPWEMGLSEV
                     HQVLVLNRLRHRIRLRVDGGIKTGRDVVIAAILGAEEYGLGTASLVAMGCILVRQCHS
                     NTCPVGICSQDPKLREKFTGTPEKVVNLFSFVAEEVREILASLGAKKLTDVIGRTDLL
                     AQVSRGSAHLDDLDLNPILAQADPGDYPRYCTLEGRNEVPDTLDAQMIKDAAALFEVG
                     EKMQLAYSIRNTQRAIGTRLSSMITRRFGMAGLQPGHLTVRLRGSAGQSLGAFAVQGV
                     KLEVFGDANDYVGKGLSGGTIVVRPMVSSPLVAHENTIIGNTVLYGATAGRLFAAGQA
                     GERFAVRNSGASVVVEGCGSNGCEYMTGGVVAILGAVGDNFAAGMTGGMAFVHDAEDR
                     FALRANGETVVWQRIETPHWEGVLKSLIEEHVRETQSRFAEQLLVDWDREIGRFWQVV
                     PKEMLSRLPAPLKVEPRKAEAGDD"
BASE COUNT         3561 a         7307 c         7416 g         3684 t
ORIGIN      
        1 gagagcgtcc gtcgcgccga cgccagcgag cgctcatcga catcgatcgc gacaccgccc
       61 gccgccccgt aggtcatcgg ccagtagctg ttgcggccca tgccgcagcc gacatcgagg
      121 aagagcttgc cgcgccaatc ctcgggcgag agatgcaccg tccagcggcg gaactgctcc
      181 tcgtattcgg cgcgcatctc cgcgtaagtg tgccattcat agccgaagcg ttccggcgat
      241 ccggcggcga cggcaggctc ggcgggtgtg gtgacgttgc tcatcggtgg tccatggcgg
      301 ttacgccgct gcccggtagc gatcgttgcc ggtgaccaga gcttgcaggc tcagccagca
      361 ccagagcagc aggcgatgat cgcgcttgcc cgcgcgatgg tcgcgccagg cgctgtcgac
      421 gaaagcgtgg ttgacgccct cgatcatggc cgcgggctgc ggcggcgcga gctcgcgcag
      481 ccacgcagcg atcggaatgc cgaatccctt tttcggccgc tcgatgatcg ccttcggcag
      541 ccagggctcg accgctcgct tcagcaggta tttgcgccgt ccgccgcggt acttgaagcg
      601 cgccggcagg cgctggcaga aagcgacgag atcgttgtcg aggaagaccg cccggctctc
      661 cagcgagttc atcatcgagg cgcggtcgac tttggcgagg atgtcgtccg gcaaatagaa
      721 gttcgtgtag tattcgaggg tcttgtcgat gacgctcttg cggctgtctt cctcccacag
      781 cgccatggcg tcgccgtaga tgtcatcgag gtgcagcggc tcgtcgaaca ggtcctgcaa
      841 ggcatcgggc tcgaccggcg ccgtccacac cgggttccac aggctcgcgt cataggaaag
      901 cccggtgagc gcgcgcctca gcttgaagtc ccagctcatg ttgcgggtgg agaggggcag
      961 cagatcggcc aggcgccgca gcccgcggtg cagcggatcc gccatcagac ggtggtaata
     1021 ccgggcgggc gccagggcac ggaacgggtc gtagcccgcg aacagctcgt cgccgccatc
     1081 gcccgacaaa gccaccgtga cctgttgccg cgtgaagcgg ctgagaagat acgtcggaat
     1141 gatcgacgga tccgccaggg gctcgtcgag ccgcttcaat acgtcgggaa tgagcctgcg
     1201 ggcggtctcc aggtcgagaa tctccaccgc cgacctcgag ccgatgaaat cggccgtctg
     1261 cctggccgcg gcggattcat cgaaggacgg ctcggcgaag cccaccgtga aggcgcgcag
     1321 gcgcgcgggc ggcaggtggc gcgcggcggc ggcgagcacg gcagccgaat cgatgccgcc
     1381 gctcagaaaa acgccgagcg gaacgtcgct catcaggcgg cggccgacgg cttgcgtcag
     1441 cagatgccgc agctcctcgg caagccgctc ctcgtccgca tcggtcaggg aatcgtcggg
     1501 ctcgatccgg aagcgccaat agcgaaagac gctggactcg ccgctcgtca ggtcgaagat
     1561 gaggcaatgg ccgccgggga gcttggcgac gccttcatag atcgcgtccg gtgccggcac
     1621 atagccgaaa gcgaagaact tctggagcgc gcgcgggctg agcttctcgc tgaacgcgcg
     1681 gtgcttcctg agggcggtga tctcgctggc gaaagcgaag agaagcgggc gctgatagta
     1741 gtagagcggc ttctcgccga accggtcgcg cgcgagaaag aggcgcttcc gacggcgatc
     1801 gtagacggcg aaggcgaaca tgccgttgag ctgggaaggg agcgattctc cccactcgcg
     1861 atagccgtgg accaggacct ccgtgtcgga atggctggag cgaaacacat ggccgcgggc
     1921 ctcgagacgc gcgcgaagct cccgatgatt gtagatctcg ccattgaaga cgacgccgat
     1981 gctcccgtcg tcgctccaca tcggctggcg cccgccgtct tggtcgatga tcgcgaggcg
     2041 gcgatggccg agatagacct ttgcctcgtc atcgacgaat tggccctcgc cgtccggccc
     2101 gcgatgcgcg agcgactccg tcatcgccgc gagatcggcc cggtcgccga gaccggagaa
     2161 accggcaatg ccgcacatga gggctagccc tcgggctcga agttgacgag ctcgcggacg
     2221 agatacggcg agcgtccctg cgattcgaaa taggtgcgca ccagcatctc cgccacgagg
     2281 cccatgagga tgctgagaat gccgacgaga aaggccatgg cggtgagcag cggcagcggc
     2341 gtcaggatca tcgacaggcc gcggaccagc ttcagataca tcatcgcgcc gaagaacacc
     2401 gacgacagga agagcgagaa gatgccgaag ccgccgaaga cgtagatcgg cttcacgaaa
     2461 taggcgtcga ggaatttcac gacgatcaga tcgaggacca ccttcagcac gcgttcgagg
     2521 ccgtatttcg atttgccggc gtgccgggcg tggtggcgga ccggcacttc gacgaccttc
     2581 ccgcccatcc agctcgcata gatcgggatg aagcgatgca tctcgccgta aaggcggatg
     2641 ccacggacga cgtcggcgcg ataggccttg agcgtgcagc catagtcgtt gaggcggacg
     2701 ccggagacgc gcgaaatgag gcggttggcg acgcggctga cgagattgcg gcgcagcggc
     2761 gcgtccttgc gatcctggcg ccagcccgag gcgacgtcgt agccttcctc gatcttagcc
     2821 aggagaagcg ggatgtcggc cgggtcgttc tgcagatcgg cgtccatcga gacgatgatc
     2881 cggccggcgg cgtgatcgat gccggccatc atcgccgcgg tctgcccgta gttgcggcga
     2941 aagtcgacga tcctgagctc cggggcatgc gccgccacgg ctttcagcgc ggcgagcgat
     3001 ccgtcggagc tgccgtcatt gacgacgatg atctcgaagc ggagcgagag cgcgcggagc
     3061 gtcggcagca atgtcgagaa gagacgctcg acattctctt cctcattgaa gaggggaatg
     3121 atgacggaga gctcgggagc cgagaacgca tcggcgacga gacgctggcg ctctccgacg
     3181 gtggccgcga aatgtttgga ccgctccggc tgcgccatca tccgagcgcc gcgccggcgc
     3241 ctccatggcg cgccttgccg cgagcgtggt taaccatggc gccgatgttc tcgaccattg
     3301 ggcgctccca gccgcgagcg atgcgcaatc ggaaatccgc cggaatgacg gcgatctcca
     3361 ggccggattt aaaggtaaaa ggggaaagga atcgtaaacg aaggccggca gaccggggcg
     3421 tgggacggcg gtttactggc ggcagtttgc tagaggacgc agagagcaca gaggatacgc
     3481 ggaggacgca gaggagaact attcctcggc gtcctctgcg ttcttcttct cttaaagtgg
     3541 cagcaaccgt tcgcgacggc gctgcacgga ggaggggatg cgcatcgctt cgcggtattt
     3601 cgcgacggtg cggcgggcaa tgtcgatgcc ttccttgagc aggatctcga cgatgcgatc
     3661 gtccgaaagg acgtcctcgt tctcggcgtc gatgagcgcc ttgatacgga agcggaccgc
     3721 ctcggccgac agcgcctcgc cgccgctcgt cgccgcgatg gccgaggtga agaagtattt
     3781 gagctcgaag atgccgcggg gcgtcgccat gtacttgctc gaagtgacgc ggctcaccgt
     3841 gctttcatgc atgccgattg cctcggcgat gtcgcgcagc accagcggcc gcagatggcg
     3901 gacgccttga cggaagaaac cctcctgctg gcggacgatc tcggtggaca ccttgaggat
     3961 ggtggtggcg cgctgatgca gcgccttgag cagccagttg gcctgctgat agcgctccgc
     4021 gagataatct ttttcggtct tgctgcgcag cgcgccgctc accgccgcgt aatagtggtt
     4081 gttgacgagc acacgcggca gcgtgtcgct gttgagttcg acgacccagc cgccggcatg
     4141 cggccgcacg agcacgtcgg gcaccaccag ttcggcgctc gccgggtcga aggcgaggcc
     4201 cggcttcggg ttgagcgctt tgagctcggc gatcatctcg gcgagatcct cggcgtcgac
     4261 attgcagagc cgcagcaagg ccggcgcatt gcggctcgcc agcagcggca gattcgcgac
     4321 cagcgcctgc atagcgggat cgaaacggtc gcgctccttc aactgcagcg ccaggcattc
     4381 agcgagatcg cgcgcgaaga cgccggccgg gtcgaagcgc tgaagaagag aaagtgtcgt
     4441 gtcgacttgc gccacctcgc aaccgagccg ctcggccacc tcggcgagat ccccttgcag
     4501 atagccggct tcgtcgacca tctcgacgag gtgggcgccg atcaggcgct cgacgggatc
     4561 gccgatgtcc acggacagct gcgccagaag atgatcgcgc agcgtcggcg gccgcgagag
     4621 cgtctggtcg aggcccggca ggtccgtggc ggctgccgca cgcggtgcgc ggccgttcca
     4681 tgccgcagga tcgccggcga gatcgagatt gccctcaccg tcggcgcccg ccgtttcgcg
     4741 ccagagcgcc acttcctcgg cgtcctccgc ggtgccgctc tcctctgcgg ccgcgggggt
     4801 cgcgccatcc gcctcggcgg gcgcgtcgtc gcgttccagg agcgggttct gctcgagttc
     4861 gctgtcgatg aacgccgtga gctcgagatt ggaaagctgc agcagcttga tggcctgctg
     4921 cagctgcggc gtcattacta acgcctgcgt ttgccggaga tcgagccgct gtgagaacgc
     4981 cataatgtgc aggcttataa cccgaagcaa caactgactg caaatatcgg gccaagtcct
     5041 tgatggacga ttaagtgtgc ccgcgccgag gtcgccacgc gtgccgaatc cggcagcaac
     5101 ttcaaggctt tttaaccaag accgcgtatc tcgttcgagg gtgcggaaag cgaccgccgc
     5161 ccgcgccacg tgacgagatg acgcatgaca gtcgagagca tcgcggtcgg tggagcggca
     5221 ggcaatacgg ccgcctctcc gatcggtgtc gccaagcttt ccaagatgca gttcgacggc
     5281 acggacctga cgccgctctg gcgcgagctc atcggcaaat atattcacga gcctgacgac
     5341 gcggcggcgc ttctcgatct cgcggtgatc gagcagctgt tcggcgaccg cgaggccggc
     5401 tgcgcgcgcc aggcggacgc cctgaaactc tgtcgcatct atcgctcccc cttccccggc
     5461 gcggcgccgt cgctccggct gctggccttc gccgtgcccg gcgacatcgg tgccaacacg
     5521 ccgctcgagt tcctgctcga gggttccgat gtcgagctcc atacgctcta cctcgtgccg
     5581 ggcgagccga tacccgcggc cgtgccgcag cacgacattg ccttcgtcgc catcggcgaa
     5641 gccgacgacc atcgcatcgc tctcgacacc atcgagacgc tgatggcgtc ctggccgcgg
     5701 ccggtgctca atcggccgga ccggatcgcc ctgctgtcgc gcgagcggct ccacgggctg
     5761 ttggcgacgg ctccggcgct cgtcattccc gcaacgacgc gtctcgatcg tgcgagcctg
     5821 gtgaagctcg gccggggcga cgtcgagctt cacgcgctga tgaccgacgc aagcttcccg
     5881 ctcattctgc ggccggtcgg gtcgcatgcc ggccatggcc tgcaaaaact cgatgatgcg
     5941 ccggcgctcg cggcttacct ggcagagcgg ccggaggctc tcttctacgc ctcgcgtttc
     6001 atcgattacc gcagccccga cgggctcttc cgcaaatacc gcatcgtctt catcgccggg
     6061 cgcccttatg cctgccatat ggcgaccacc gaccagtgga tgatctacta cctcaatgcc
     6121 aacatgaagg agagcgccgc gaagcgcgtc gaagaggagc agttcatggc gcgcttcgac
     6181 accgatttcg cgcgccgtca tgcggcggct ctcgctgccg ttgccgagcg cgtcggcctc
     6241 gactatttcg gcatcgattg cgccgaaacc cgcgatggcc gcctgctgat cttcgaggcc
     6301 gacattgcga tgatcgtcca tgccatggat gatccgcaga tctttccata caagggtccg
     6361 cagatgcgca aggtcttcga tgccttccgc gcgatgctgc gcgagcgaag cgccaaggcg
     6421 ctttcatgag cgacgagacg ctcgatcccg acgactggga gggcgtcagg caggtatttc
     6481 acgaagcggt cgatcgcgcc atcgatcacc tgcgcgatat ccgccggaag cccgtctgga
     6541 cggcgacgcc ggaggccacg aagaagaagc tgcgcgcgaa gctgccgcac gcggccgagc
     6601 cgctcgatca tctcttgtcg agctttgtgc aggacatcct tccctatggc accggcaaca
     6661 tccatccgcg cttcttcggc tgggtgcatg gcagcggcaa cgtcgccggc gtgctcggcg
     6721 agatgctggc gtccttcatg aactgcaacg tcggcggccg cgatcatgtc gcggtctatg
     6781 tcgagcgcca ggtgatcgac tggtgcaagg agatcttcgc ctacccggcg acgagcagcg
     6841 gcctgctgac gagcgggacc tccatggcca ccgtaatcgc gctcgctgtc gcgcgcttcg
     6901 ccaaagctgg cgcgtcggtg cggacagacg gcgtcgccgc ggcggcccgg ccgcttactg
     6961 gctatgcgtc gagcgaagcc cacggctccg tcggcaaggc cttcgacctt ctcggactgg
     7021 gacaaggcgc gcttcgcgcc attcccgtcg atgccgactt ccgcatgcgc ctcgccgacc
     7081 tcgagcgaca ggtcgcgcaa gatcgggcag cggggcgaat gcctgccatc gtcgtcgcct
     7141 cttgcggcac ggtgaacacg ggtgccatcg acgatgtcgc cgccatcgcc gccttctgtc
     7201 gcgcgcaagg gctttggctc catatcgacg gcgcgttcgg cggtgcggcc gtgctcctgc
     7261 ctgagtttcg cgcgaagctc gcggccatcg ccgaggcgga ttcgatcgcc ttcgatttcc
     7321 acaaatggct tcacgttccc tatgacgccg ggtgcgtcct ggtgaaggac gcggcggcgc
     7381 atcgcgcggc cttcgcgatg cggccggatt atctcgccgc cgcgcatcgc ggccttgctg
     7441 gcggcgagcc ctggttttgc gattacgggc ccgagctctc gcgcggcttc cgcgccctca
     7501 aagtgtggtt caccatcaag gcccatggca tcgatcgcct gtctgcggcg atggcgcgca
     7561 attgccgcca agcgcgcctg ctggcggaga cggtgaccgc cagtgccgat ctcgagctcc
     7621 tcgccccggt cgagctcaac atcgtgtgct tccgtttccg cgcggcaggg gtggcggacg
     7681 ccgcgttgag ccggctcaat gaggaaatcg tcgccgatct gcaggaggag ggcatcgccg
     7741 cgccctccac ggccaggatt ggcgggcgga cggccatccg cgtttgcctg accaatcatc
     7801 gcaccggcga cgaggatctc gcgctgcttc tggacgcaat ccgccgcctc ggcaacgagc
     7861 gcctcgcccg agcggagacg cgatgaccgc tccgccgttc cgccagctcg gcgagatctt
     7921 cgccccgctc gaccatatcc tcgcatcggg cggtgacgcc cgcctgcaca tcgatcccat
     7981 caccggcctc aacggctatg gctgccggcc ctaccctcgc ccggacgcct ccactttcgc
     8041 ctcctcgacg gcgacctcga tctccgagcg cgcctatgcc tgcgccgagg ccgagcacca
     8101 cgacctgatc cgcgccggcc tgcgtcaggg cttcgaaacc gcggtggacg accgcttcga
     8161 gcagctcaga gacgagctca ggaccgtcct cgatctcgcc gacagcggca tcgagatcgt
     8221 cttctcgccg tcaggtaccg actcgacgct gcaggctctt ctggtggcgc gcgccatgct
     8281 cggcacgccg ctgaccagcg tcattgccgc cgccgacgag accggcagcg gtgtcggcga
     8341 cgcggcctcc ggccggcact tcagcgcgct cacatcgcaa gccgtgatcg tcaaacgcgg
     8401 cgatcccatt caaggacttg ccgagggcgt gcgccgtatc gccattccct tgcgcgacga
     8461 acgcggccat gcccgcccgc ccggcgtcat ggacgagcag gtgaagcgtg ccgtcgcggc
     8521 cgcggcggcc gccggggagc gcatcttgtt gcatgccatg gatcactcga agctcgcggc
     8581 gcagtgcccg agcctcgact gcttgcgcgc cgtcgccgac gcggcgcccg gcgcggtgca
     8641 gatcgtcatc gatgcctgcc agatgcgcat cagccggacc cgcctcaaac ggcatttggc
     8701 cgaagggcat atggtgctca ttaccggttc gaaattcttc accgggccgc cgttcagcgg
     8761 cgctctgctc gtcccgggcg cgttgtcgca gcgcatggcc gcgctcgacg tcgtgccggc
     8821 ggggctggcc gactacagca gccgcagcga ctggccgcgg gcgtggcaag gattgcgggc
     8881 ggcgctgccg gcacgggtgg cgctcggaca gctgctgcgc tggattgcgg cactcggcga
     8941 gatgcgcgcc tatttcgcgg tgccgccgtc gttccggtcc ttcgcccttg cccgctttgc
     9001 gaccgtcgtc ccggccctga tcgccgccac gcccactcgc gaattgctgg ttgatcggag
     9061 ttgcggggat ggccatgcca ccgacgacga ggagatggcg tcgccaacca tcttcccctt
     9121 ccggctccgt cgcgccgggc ggcttctctc gctcgaagag gcccgtcttg tctaccgcgc
     9181 gctcaacgcc gatgtgacgg cgatgctgcc ggcggctgcc accgcggccg accgccagct
     9241 tgcggcacag ctctgccatg tcgggcagcc cgtcggcctt gccgatccgc cgcgcggaac
     9301 gagcggcgcg ctccgcatca gcgccggggc ccgtttcgtc tcggagagct ggtcgcccgc
     9361 cggcgagagc gtggccatgg cgaatctgga cggggaattc gcgcagattc gcgcgattct
     9421 cgccaagatc gatctactgc tccgccattt cgaggcgctc ggacgctggg acgcgcggcg
     9481 caccgagcgc aaggtggcgc gcgcggggtg agtccctgtt tctccccctc tcccgcaggc
     9541 gggagagggc gagttgctgc ggcttgagac gggctctcgc ggttgctgcg caaccgctgc
     9601 cgcccgctcg cgacgacgct tcgcgtcgcg cggcttacag gctgaacttc tcgccgagat
     9661 agacgcggcg cacatcggcg tgagagacga tctcggccgg cgagccctcc atcagcaccc
     9721 gcccctcatg gaggatgtag gcgcggtcga cgatctccag tgtctcacgc acgttgtggt
     9781 cggtgatgag cacaccgatg ccgcggtcct tcagatgcgc gacgagatcg cggatgtcgc
     9841 tcacggcgat cggatcgatg ccggccaagg gctcgtcgag caagatgaaa tgcggctggg
     9901 tcgcgagcgc gcgcgcgatc tcgacgcgac gccgctcgcc gcccgagagt gccagcgccg
     9961 gggcgcgccg cagatgcgtg atcgagaact cggcgaggag ttcgtcgatc atcgtctcgc
    10021 gcactgccgg aaccggctcc gccacttcga ggacggcacg gatgttctgc tccacggtga
    10081 ggccgcggaa gatcgacgcc tcttgcggca gatatccgat gcccagccgc gcgcggcgat
    10141 acatcggcag ctcggtgatg tcctggctgt cgagcgcgat ggtgccgacg tcaggcgcga
    10201 tgagtccggt gatgatgtag aaacaggtgg tcttcccggc gccgttgggg cccaggagtc
    10261 cgaccgcttc gccgcgctgc accgaaacat tgacgtcgcg cagcaccgga cgcttcttga
    10321 agctcttgcc gagattggtg gcgacgagcc cgcgattgtt ggcgacgagc cgcggcacgg
    10381 cgccggggcg gccggcggag cgcggcgcat cggcggattc gggcgtcatt gtggcggcgc
    10441 ggccggcttc gaggccggcc cgtccgattt tgctgccacc gcgtcgggtt tcgccccagc
    10501 cgcttcgggt ttcggccgcg gcaccagcag gccgtggacc tgcggccgcg gcccttcgct
    10561 cacctggtcg cccggcggcg cgctcagcag gcggctcaca ttgctgttga ggtcgaccac
    10621 gccatactgg ccgcggagct cgttgtcgcc acgcgtcagg gtcacgttgc cggccagcgt
    10681 ggcgattccg gtatcgacgt tgtagacgcc ggtgtcgccg cgcccgatct ggccttgcga
    10741 cgagacgagg acgttgcctt gcgcgtcgat gcggctgata tgcgaggacc cgtcgggtcc
    10801 cttggtcaca tccgcggtca gcacgtcgcc gcgcagcgcc ttgccgcctt cctggaccgc
    10861 gaccgcgtcg ccgcgcgcca cggcgcgctg cttgctgtcg taccattcga ggctgtcgcg
    10921 cgccgtcacg gtgtcccgcg gcgtctcgag cttcaaatgc ttgcccgtcg ccacgagcag
    10981 cgtctgatcg acgtcataga tggcatgatc gcagtagacg gtctgcgtct ccgtggcgat
    11041 ccgcacattg ccgtcggcct cgaggcgata gatctcggtg gagctgccgc tcgtcgggtc
    11101 gcccggcttg ccgccgcccg caggcgacgc cggcggcggt ggcggcgcgc tgttgccgtt
    11161 cgtggcgttg gcgaccggcc gataataggc atagagcgtg tcgccgaaga cagtggcctg
    11221 tccgcgcgtc gccttggcgt ggccgcgcgc gatatagacg cgattattct gttgccattc
    11281 gatgtcttgc tccgcctcga tggtgatcgg cttgccattg tcctggctgt tgaggccaag
    11341 cccgaaattc tgggcttgcg tgacaggcgc gagcaggaga cccggagcga aggcaagcag
    11401 aatcgcgagg aagcgcgacg tcactggccg gccttttcat gcggcgcgag atcgagacgc
    11461 gaacggcccg tgaagatgac gacgtcgccg tggtcgagga tacggaaccc ctggccggtc
    11521 gcatgcccga agggaccttg gccctcgatc ggctcatgcc cctcggcggt gccgttcgcc
    11581 atgtcgacat gggcgctttc gctgtggaac tcgttgccct tgtcctgaaa gagctgcacg
    11641 tcgccgaaaa ggtcgagcag ctgcgcctgc ggctgataga ggcccgtgcg cgacgtgact
    11701 tcgaaccagc ccccggtcag cgtcgtcaaa tcgcctttgg gtccctcgag ggcgacgatg
    11761 tcggcggcgc cggggttctg gcgcgcgatc tcggccgtga tgacgtaagg ccgctgctgg
    11821 cggtcgatgc cgctgtaatg cgcgtgcacc atgcgcagat cgtgcgcttc gcgcaggtcg
    11881 atccgcggca gggcgaaatg gacatgctcg atcgcctcct cgatgcgcgg ccagaccgcc
    11941 accaggacga tcagcgacac cgcaatcacg ggaaagatgc gcttggccag gctgacgaag
    12001 cggctatagc gccgactgaa gacggcgcgc cggcgcgggc ggccgacctc gccggcgccg
    12061 aggaacgtgt cgggaggggc cgtcacgcca cgccttcccg gaggcagtca tggaggtcga
    12121 ggatgccgag cggccgctcc gcctcatcga ccacgaacaa attcttgatc gcgcccggcg
    12181 gcgcgtgcat tcgcccgaga ccttcggcgg ccagcgcttg cggccggatg gtcttggggc
    12241 ccttggtcat caccgcgtcg acggtgctgc gcagcaggtc atcggccata tgccggcgca
    12301 ggtcgccatc ggtgatgatg cccacgaggc atcccttggc gttggtgatc ccgacgcagc
    12361 cgagcccctt ttcggacatc cgcaggatgg cttcggccat cggcgttccc gacgggacca
    12421 gcggcacggc gtcccctgtc cgcataacat cggcgacgcg cagcagctgg cggccgatct
    12481 ggccgccggg atgcaacacc tggaaatcgc tcgccgaaaa tcctttgcgc tcgagcagtg
    12541 ccacggccag ggcgtcgccc agggccatca tcatggtggt cgaggtcgtg ggggcgagcc
    12601 ccatcgggca ggcctcttcg gtcgcgggca gcagcagggt cacatcggcc gcctcggcaa
    12661 gcgtgctgcg gccgcgccgg gtcatggcga ccagcgggat gcgaaaacgc cgcgtatagg
    12721 cgacgatgtc gccgagttcg gccgtgtcgc cggaattcga gagcgccagc accgcgtcgc
    12781 ccccggcgat catgccgaga tcgccatgac tggcctcgcc cgggtggacg aactgcgcgg
    12841 cggtgccggt cgaggaaaac gtggcggcaa tcttccgcgc cacatggccg ctcttgccca
    12901 tgccggtgac ggtgacccgg cctctgactt gcgccagctt ttcgaccgct gcggcgaagg
    12961 tgccgttgag ttcctcggcg agcgcctcga gaccggcgat ctccgtgcgc agaacgcggc
    13021 gcgcggcatc gatatccgga gcgttgctca gcatgagcga gccaacatat cctttcagct
    13081 gaatcgacca gccgccgggc cggaagcggg ggccgcggtg cgtgccgcat gatacggcgc
    13141 cactatgatc ccgcatccct aaaagtcaat gaaaccgggc atcaatgggc gaagatgtcc
    13201 tcgtgggccc aaccgccgag gtcgaggagt gcccgcgtcg gcagaaactc gtagcaggcc
    13261 tgcgccacgg cctgccggcc ctcccgcgcc agcatctggt cgagcttggc cttgagtgcg
    13321 tggagatgca gcacatcgga ggccgcatag cggagctgct cgtcggtgag ctcggccgcg
    13381 ccccaatcgg aggattgctg ctgcttcgag agctcgaccc ccaggaggtc gcgacacagg
    13441 tccttgagcc cgtggcggtc ggtgaaggtg cgcaccagcc gcgaggcgat cttggtgcag
    13501 taaagcgggc gcggcattac gcccaggtgg cgctccatga cggcgatatc gaagcgggcg
    13561 aaatggaaga tcttgaggac ctcgggatca gccagcaaaa ccttgaggtt gggcgcgtca
    13621 tagacgccgg ccgcgaattg gacgaggtgg cagacaccgt ctccggccga gagctgggcc
    13681 aggcaaaagc ggtcgcgatg gggatcgagc cccatcgtct cggtgtcgat ggcgacgctc
    13741 cggccgaaat cgaggcccgg gggaaggtcg ccgtgatgaa gatggatgct caccgaggac
    13801 gatcactcgc ggaaattggt gcccaggaaa ggactcgaac cttcacgcct cgcggcacac
    13861 ggacctgaac cgtgcgcgtc taccaattcc gccacctggg ctggccggcc ctgtgcttag
    13921 ggaagggggg cggcgatgtc aaacgattcg ctggcctccg gcgctgggaa agtcgcgcag
    13981 agtctccacg ttaccgcgtt gctgtgataa gtgtctcacg gcaaaagggg gcacacgatg
    14041 cggtatcagc gggtcctgat cgtcggcggc accggattca tcggccgcta tgtggtgaag
    14101 cgtctggccg cacggggtac cgtcatcgcg gtcgtcagtc gcaatgccga cgccgcgggt
    14161 tttctccgcc ccttgggcga cgtcgggcag atcgcgctca tcaacgccgg cctcggcaac
    14221 gagcggcgcc tgcgcgcgct gctcgaagag ggcatggacg ccgtcatttg ctgcgccggc
    14281 gtgctttact cgcgcggccg ccagactttc gcggaagtcc accacgaggg tccggcccgg
    14341 ctggcgcggc tcgcgacagc ttccggcgtc aaacgcttcg tccatgtctc ggcgctcggc
    14401 gccgacgctg cctcgccctc ggtctatggc cgctccaaag cggccggcga agccgccgtg
    14461 cgcgcggctt ttccggaggc ggtgatcttc cggccgtccg tcgttttcgg ccccgaggac
    14521 gatttcttca atcgcttcgc cagccttgcc cgctattcgc cggtgctgcc gctcatcggt
    14581 ggcgggctga cgcggttcca gccggtctat gtcggcgatg tggccgatgc cgtggccgcg
    14641 gccctcgatc gccacgattc cgccggccgc atcttcgagc tcggcggccc gctggtctac
    14701 agcttcaagg agctgatgga gctgttgctg cgcgagatcg accgccggcg agcgctggtc
    14761 agcctgcccg tcccgctggc gcagctcatg gccttcttcc tcgaatggtt gccgagcccg
    14821 ccgctcaccc gcgatcaagt caggctgctc cagaaggaca acgtggtcga gccgggcgtg
    14881 cccgacctcg gctcgctcgg ggtgacgccc acaagcctag agcttgtcct tcccacgtat
    14941 ctcgaccgct tccgcagagg cgggcgattc ggactggccc gcaccgcgcc cgccggtcct
    15001 ctgaacgaat agcgtccgca agcatatcgc cgattttgcc ccgattgagg caaggctgcc
    15061 gcttgactga gcaatatagt actgaaacga gactaatata gctctctcgg cctgccgaaa
    15121 gaggcgcata gccgacatgc aggcttacac gcgctgaaaa ggtaaaatat tttgccctaa
    15181 aagcgttttc gtgcttcact ttggatgctg gagcaacaga gttgtcgtca tgaagccggg
    15241 attgtcgcaa ttcgtgggct tggagaaggc gatgccggag aagcggccgg cggagcagcg
    15301 ccgccacgac ttcaaggaaa ttcacggcga gttcgatccg gcgcgcgcgc gccagcaggc
    15361 gagccgctgc tcgcaatgcg gcgtccctta ctgccaggtg cattgcccgc tcggaaacaa
    15421 catcccggac tggctgatgc tgaccgccgc cgggcgcctc gaggaggcct atgaggtggc
    15481 cgcggccacc aacaacatgc ccgaggtctg tggccgcatt tgcccgcagg accggctttg
    15541 cgaaggcaat tgcgtcatcg agaagggctt ctcgtcggtc accatcggct cggtcgagaa
    15601 gtacatcacc gagacggcct tcgccaaagg ctgggtgaag ccgccgctcc cgcggcacga
    15661 gctcggccaa tccgtcggca tcatcggcgc cggcccggca gggctcgcgg cggccgagca
    15721 gctgcgccgc aagggctatg ccgtccatgt ctatgaccgt tacgaccgcg tcggcgggct
    15781 cctcgtctac ggcattccca atttcaagct ggagaagcgc gtcgtggcgc ggcgccacga
    15841 gctgctggtc ctgggcggca tcatcttcca tctcgatttc gaggtcggac gcgacgccga
    15901 tctggcgacg ctgcgccagc gccatgatgc ggtgctcatc gccaccggcg tctaccaggc
    15961 gcgtgccatg gcatgcccgg gcatcggcct cggcaatgtg gtggcggcgc tcgactacct
    16021 gacggcgtcc aaccgcaagg ggctgggcga tgccgtcccg gccttcgaca gcggtgcgct
    16081 gaatgcggtg gaaaaggatg tcgtggtggt gggcgggggc gataccgcga tggactgcgt
    16141 gcgcacggcg gtgcgccagg gtgcgaaatc ggtgcgctgt ctctatcgcc gcgaccgcaa
    16201 gaacatgccg ggttcgcagc gcgaggtgcg gcacgccgag gaggagggcg tcgagttcgt
    16261 ctggctcgcc gcgcccgagg cgctcctggg cgaaagcgag gtgcgcgagg tgcgcgctca
    16321 ccgcatgcat ctgggcgtcg ccgattcgag cgggcgccag gtgccgcagc cgctgcccgg
    16381 cagtcacttc atgcttcccg ccgatctcgc catcctggcg ctgggcttcg atcccgagga
    16441 ggtgccgcgt ctcttcggcg ccccggagat cgcgctgcgc cccgacggca cgatcaagat
    16501 cgatttccgc agcatgatga cgtcgctcga cggcgtgttt gccgccggcg acatcgtccg
    16561 cggcgcttcg ctggtcgtct gggccatccg cgacggccgc gacgcggcgg agcagatcca
    16621 tcgctacgtg cagcagaagg cagccgcggt ggtcgcggcg gcggagtgaa ggcgatgctg
    16681 cgtcttcacg acaatgccag ctcgggcaat ggctacaaag tgcgtctcct cctgacgctg
    16741 ctcggccggc cgttcgggcg catcgagtac gacgtcgacc gtggtgcgac gcgcacggcc
    16801 gaattcctca gcaagaatct caacggccgc attcccgtgc tcgagctgga ggatggccgt
    16861 tttctcgccg aatccgacgc catcctggtc tatctcgcgg agggcacgcg cttcctgccg
    16921 caggaccgct acgagcgggc gctggcgctg caatggctgt tcttcgagca gtacagtcac
    16981 gagcccaaca tcgcgacatc gcgctattgg atcacgcatg ggctcttgaa tgacgagcgt
    17041 cgcgccctgc tcgacggcaa gcgccgccag ggccacgcgg cgctcgcggt gatggagcgg
    17101 catctcgaag gacgccgcta tttcgtcggc gagcgctact cggtcgccga catcgcgctc
    17161 tacgcctaca cgcatgtcgc ggacgaaggc ggcttcgatc tcgccggcta cccggccgtc
    17221 cggaaatggc tcgaccgcgt cgcgagcgag cctggccata tcccgatcac gaaggtctga
    17281 gtgcaatgac tgagcatcag cccccctcac cctcccaatg ggccgacgac tatcgacgga
    17341 acgcggcgca tctcgaggcc gcggggctct atgaccccgc cgaggagcat gatgcgtgtg
    17401 gcgtcggatt cgtcgcgtcg ctcgatggca aggcgcggcg cgcggtggtg gcggcggcga
    17461 tcgatgcgct gcgcgccgtg tggcatcgcg gcgccgtcga tgccgacggc aagaccggcg
    17521 atggcgccgg catccatgtc gagattcctc aggagttctt ccgcgcccat gtgcgccaca
    17581 ccggtctcga gccggggccg ggccatctcg cggtcggcat ggtgtttctg ccgcgcaccg
    17641 atctcgacgg gcaggagcgc tgccgctcca tcgtcgaagc cgagatcttg aacgccggct
    17701 atgcgatcta tggctggcgc caggtgccgg tcaatatcga catcatcggc gagaaggcga
    17761 acgcgacgcg ccccgagatc gagcagatca tgatcgccaa tgcgcgcggc gccgaggtcg
    17821 agcgtttcga gctcgatctc tacgtcatcc gccgccgcat cgagaagcgc gtcatcgccg
    17881 accaggtcaa ggacttctat atctgctcgc tgtcctgccg ctcgatcgtc tacaagggca
    17941 tgttcctggc ggagcagctc acggccttct atcccgatct cgccgacgag cgcttcgtct
    18001 cgcgtttcgc catctatcac cagcgctact cgaccaacac cttcccgacc tggccgctgg
    18061 cgcagccctt ccgcatcctc gcccataacg gcgagatcaa tacggtgcgc ggcaacatca
    18121 actggatgaa gagccatgag acgcgcatgg cgagcgacgt cttcggcgac gccatcgagg
    18181 atctgaagcc ggtgatccag gtgggcggct ccgacagcgc ggcgctcgac gccgtcttcg
    18241 agctcctggc gcgctcgggg cgcgtgctgc cgatggtcaa gaccttgatg gtgcccgagg
    18301 ccgcctccga cgcgcggctc atgcccgaaa cgcatcgcga catgtacagc tattgcaact
    18361 cggtgatgga gccctgggac ggccccgccg cgctggccat gaccgacggg cgctgggttc
    18421 tcggcgggct ggaccgcaac ggtctgcgtc ccatgcgcta tgccgtgacc agcgacggcc
    18481 tcatcattgc cggctcggaa gcggggatgg tgcgcctcga cgagacgacc atcatcgaga
    18541 agggccggct cgggccgggt cagatgatcg gcgtcgatct cgcctcgggc aagctccacc
    18601 gcgaccgcga gatcaaggac aggcttgccg cgggccggcc cttttccgcc tgggtcaagg
    18661 gcatcacccc gctcgacagc ctcatccggc cgagccatgg cgagaaggcg gagctcggcc
    18721 gcgacgagct caggcgccgc caggtggcct tcggctggag cctcgaggat ctcgagctga
    18781 tccttcagcc catggccgag gacggcaagg aagccgtggg ctcgatgggc gacgacacgc
    18841 cgctcgcggt gctctcggac cattaccgcg gcctccatca tttcttccgc cagaatttca
    18901 gccaggtcac caacccgccg atcgacagtc tgcgcgagcg ccgtgtcatg accttgcgca
    18961 cccggctcgg caatctcggc aacatcctcg acgaggatgc gagccaatgc cgcctgttgc
    19021 agctcgactc gccggtgctg tccaacgccg agttcgcggc gatgcgcgcc tatatgggcg
    19081 agaccgcggc cgagatcgat tgctgtttcg aagcgcaagg cggcagcgcc gccctcaagg
    19141 cagcgatccg ccgcgtccag caggaagccg aggacgcggt gcgcggcggc tgcacccatg
    19201 tgatcctgag cgattccggc atgtcggcga agcgcgcggc gatccccatg attctcgcgg
    19261 cgggcgccgt ccattcccat ctcgtgcgcc agcagcttcg caccttcaca tcgctcaacg
    19321 tccgctcggg cgagtgtctc gatgtccatt attttgcggt gctgatcggc gtcggcgcca
    19381 ccacggtcaa cgcttatctc gcgcaggaga cgatcaccga ccgccaccgg cgcggcctct
    19441 ttgcgccggg atcgagcgtc gaggcggcgg tcgagcgcta caagcaggcg atcaatgacg
    19501 gcctgctcaa ggtcatgtcg aagatgggca tcgccgtgat ctcgtcctat cgcggcgcct
    19561 acaatttcga ggcggtgggg ctctcgcgca cgctggtggc cgagtatttc ccgggcatga
    19621 cgtcgcgcat ctcggggatc gggcttgccg gcctcgagat caagacgctc gagacgcatc
    19681 gccgcgctta tgccgaggac gtcatcgctc tgcccgtcgg cggcttctac cgctatcgcc
    19741 ggggcggcga gcttcatgcc ttcgaaggca ccctgatcca cacgctccag accgccgtcg
    19801 agagcgattc ctacggcgcc tatcggcgct acagcgaggg catgcgcaag ctgccgccca
    19861 tccatctgcg cgacctcctc gacttccggc cgaagcggcc ggcggtctcg gtcgacgagg
    19921 tggaatcgat caccgaggtc cggaagcgcc tggtggcgcc cggcatctcg ctgggggcgc
    19981 tcgggcccga ggcgcatgag actctgtcca tcgccatgaa ccgcatcggg gcgcgctcgg
    20041 acagcggcga aggcggcgag gatcccgcgc gctatcgtcc gcgcgccaat ggcgacaacg
    20101 cgtcctcggc gatcaagcag atcgcctcgg gccgcttcgg ggtcaccgcg gagtatctca
    20161 acaattgccg cgagatcgag atcaagatcg cgcagggcgc caagcccggc gagggcggcc
    20221 agctgccggg cttcaaggtc acggagatgc tggcacggtt gcgccattcg acgcccggcg
    20281 tgtcgctggt ctcgccgccg ccgcatcacg acatctactc gatcgaggac ctggcgcagc
    20341 tcatctacga cctgaagcag atcaatcccg aggccaaggt ctgcgtgaag ctggtggcgc
    20401 gctccggcat cggcacgatc gccgccggcg tcgccaaggc caaggccgat gtgatcctga
    20461 tctcgggcca tagcggcggc accggcgcga gcccgctctc ctccatcaag catgcgggcg
    20521 tgccctggga gatggggctc tccgaggtgc atcaggtgct cgtgctgaac cggttgcgcc
    20581 accgcatccg cttgcgcgtc gatggcggca tcaagaccgg ccgcgacgtg gtgatcgccg
    20641 ccatcctcgg ggctgaggaa tatggcctcg gcacggcgtc gctcgtcgcc atgggctgca
    20701 tcctggtgcg gcaatgccac tccaacacct gcccggtcgg gatctgctcg caggatccga
    20761 agctgcgcga gaaattcacc ggcacgccgg agaaggtggt gaacctcttc agcttcgtcg
    20821 ccgaggaagt gcgcgagatc ctcgccagcc tgggcgcgaa gaagctcacc gacgtgatcg
    20881 gccgcaccga tctcctggcg caagtgagcc gcggcagcgc gcatctggac gatctcgacc
    20941 tgaacccgat cctggcgcaa gccgatcccg gcgactatcc gcgctactgc acgctcgagg
    21001 gccggaacga ggtgccggac acgctcgacg cgcagatgat caaggacgcg gcggcgctct
    21061 tcgaggtcgg cgagaagatg cagctcgcct acagcatccg caacacgcag cgcgccatcg
    21121 gcacgcggct ctcctcgatg atcacgcggc gcttcggcat ggcggggctg cagcccggcc
    21181 atctgacggt acgcctgcgc ggctcggccg gccagtccct gggcgctttc gcggtgcagg
    21241 gcgtgaagct cgaagtcttc ggcgatgcca atgactatgt cggcaagggc ttgtcgggcg
    21301 gcacgatcgt ggtgcgtccg atggtctcga gcccgctcgt cgcgcatgag aatacgatca
    21361 tcggcaacac cgtcctttat ggcgcgaccg ccggccggct cttcgcggcg ggccaggccg
    21421 gcgagcgctt cgccgtgcgc aactcgggcg cctcggtcgt ggtcgagggc tgcggctcca
    21481 atggctgcga atacatgacg ggcggcgtcg tcgcgatcct cggcgcggtc ggcgacaact
    21541 tcgcggcggg catgaccggc ggaatggcgt tcgtccacga cgcggaagac cggttcgcgc
    21601 tccgcgcgaa cggcgagacc gtggtgtggc agcgcatcga gacgccgcac tgggagggcg
    21661 tgctcaaatc gctgatcgag gagcatgtgc gcgagaccca gtcgcgcttc gccgagcagc
    21721 tgctggtcga ctgggaccgc gaaatcggcc gcttctggca ggtcgtgccg aaggagatgc
    21781 tgagccggct gcccgcgccg ctgaaggtcg agccgcggaa ggcggaagcg ggagacgatt
    21841 aaggtcgaaa cccataaagt gtgcccgccg cggctgcttc gggcgcatct ccgacgaacg
    21901 gctcgatgca gcaagtctga aatcggagcg gcagcagtcg caggcacgct cctttaccct
    21961 cgaagagc
//
LOCUS       DASYNS010000459         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3549215, whole genome shotgun sequence.
ACCESSION   DASYNS010000459 DASYNS010000000
VERSION     DASYNS010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3549215"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..121
                     /locus_tag="VGP50_14400"
     CDS             17..121
                     /locus_tag="VGP50_14400"
                     /inference="COORDINATES: protein motif:HMM:NF045145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028621.1"
                     /translation="MCLLAYRMTQVRLMLRQYPWLYEAGGPFSWRERG"
     gene            207..1316
                     /locus_tag="VGP50_14405"
     CDS             207..1316
                     /locus_tag="VGP50_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV8028622.1"
                     /translation="MKRAFLLAAALALVASMASAADINVAMVGPITGQYAAFGEQMRR
                     GAEMAVIDINAKGGVLGHQLNLMVGDDACDPKQAVNVANQMANSGVVFIDGHYCSSSS
                     IPASSVYADNGLLQITPASTNPALTDDAAEKGWDNVFRVCGRDDAQGRTAGRFIAAHY
                     KDKAVAILHDKSTYGKGLADETKKALNATGIKEVMYEATNADDKDFSALISKMKQANV
                     ALIYLGGYHTEAGLLVRQAHEQGLKAVLMAGDALATDEFWTITGAAGEGSLMTFAPDP
                     RGLPAAKAVVDKFKQQGYEPEGYTLFTYAAMQVFAEAATRAKSEKLDAVSKAMHSGKF
                     DTVIGTVGFDKKGDVIGPDYVVYRWHDGKREELKM"
     gene            1325..1729
                     /locus_tag="VGP50_14410"
     CDS             1325..1729
                     /locus_tag="VGP50_14410"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV8028623.1"
                     /translation="MDDLERLQQSRLPFAEALGVRYTSAAKERVTAELLVREDLCTRP
                     AVLHGGAIMAFADTLGAAATILNLPEGAWTTTIESKTNFLAPAPVGGTVVGETTPVHR
                     GRRSMVWQTRVSTAEGRLVALVIQTQMVLEQR"
     gene            complement(1794..>2189)
                     /locus_tag="VGP50_14415"
     CDS             complement(1794..>2189)
                     /locus_tag="VGP50_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEV8028624.1"
                     /translation="VVAAYVSNNTLPTGQLAEVISAVYKSLRALDGQTLESQSEPLKP
                     AVAVRKSITPDYLVCLEDGKKLKMLKRHLRSTYSMTPEDYRQKWGLPADYPMVAPNYA
                     EQRSAFAKKIGLGRGTGRQAGKRVAAAKK"
BASE COUNT          381 a          710 c          737 g          361 t
ORIGIN      
        1 ggcgatcctg cttttcctgt gcctcctggc gtaccggatg acgcaggtgc gcctcatgct
       61 ccggcagtat ccctggctct atgaagcggg gggacctttc tcctggcgcg agcgcgggtg
      121 agcgccgcag gcttacgccc gcgccaaacg cctgctagag tcgcggcccg tgcggggcgc
      181 ttccgccagc cagggagttc gtgacaatga aaagagcatt cttgctcgct gcggccctcg
      241 ctctcgtcgc gagcatggcg agtgcggccg atatcaatgt tgccatggtc ggtcccatca
      301 ccggccagta cgccgccttc ggcgagcaga tgaggcgcgg cgccgaaatg gccgtcatcg
      361 acatcaacgc caagggcggc gttctcggac atcagctcaa cctcatggtc ggcgacgacg
      421 cgtgcgaccc caagcaagcc gtcaacgtcg ccaaccagat ggcgaacagc ggcgtcgtct
      481 tcatcgatgg ccattactgc tcgtcgtcct cgatcccggc ttcctcggtc tatgccgata
      541 atggcctgct gcagatcacg ccggcctcga ccaatccggc gctcaccgac gacgcggcgg
      601 aaaagggctg ggacaatgtg ttccgcgtct gcggccgcga cgacgcgcaa ggccgcacgg
      661 ccggccgctt catcgccgcg cattacaagg acaaggccgt ggcgatcctc cacgacaagt
      721 cgacctatgg caaaggtctc gccgacgaga cgaagaaggc gctcaacgcc accgggatca
      781 aggaggtgat gtatgaggcg accaacgccg acgacaagga tttctcggcg ctcatcagca
      841 agatgaagca ggcgaatgtc gcgttgatct atctcggcgg ctatcacacc gaggccggat
      901 tgctggtgcg ccaggcgcat gagcagggcc tcaaggccgt gctcatggcg ggcgacgccc
      961 ttgccaccga cgagttctgg acgatcaccg gcgccgcggg cgagggctcg ctcatgacct
     1021 tcgcgcccga tccgcgcggc ctgccggccg ccaaggccgt cgtcgacaag ttcaagcagc
     1081 agggctacga gcccgagggc tacacgctct tcacctatgc cgccatgcag gtgtttgccg
     1141 aggccgcgac gcgggcgaag tcggagaagc tcgatgcggt ctcgaaggcc atgcacagcg
     1201 gcaagttcga cacggtgatc ggaacggtcg gcttcgacaa gaagggcgat gtgatcgggc
     1261 ccgactacgt cgtctatcgc tggcatgacg ggaagcgcga agagctgaag atgtaggagg
     1321 ggcgatggac gacctcgaac gcctccagca atcgcggttg cccttcgccg aggcgctggg
     1381 cgtccgctac accagcgcgg cgaaggagcg cgtcaccgcc gagttgctcg tgcgcgagga
     1441 tctctgcacg cggccggcgg tgctccatgg cggcgcgatc atggcgtttg ccgatacgct
     1501 gggcgccgcc gcgacgatcc tcaatctgcc cgagggcgcc tggacgacga ccatcgaatc
     1561 caagaccaat tttctggcgc cggcgcccgt cggcggaacc gtcgtcggcg agaccacgcc
     1621 cgttcaccgc ggccggcgca gcatggtgtg gcagacccgt gtctccacgg ccgaggggcg
     1681 cctcgtcgcc ttggtgatcc agacgcagat ggtgctcgag cagcgctgac gacgttaacg
     1741 aaaaccgcgc cccccggaga gggacgcggc gttcgaaggt gcggcggacg gagctatttc
     1801 ttggcggcgg cgacgcgctt gccggcctgg cggccggtgc cgcggccgag accgatcttc
     1861 ttcgcgaagg ccgagcgctg ctccgcgtag ttcggcgcga ccatcggata atcggccggc
     1921 agcccccatt tctggcggta atcctccggc gtcatgctgt aggtcgagcg cagatgccgc
     1981 ttgagcatct tgagcttctt gccgtcctcg aggcagacga ggtagtcggg cgtgatcgac
     2041 ttgcggaccg ccacggcggg cttgagcggc tcggactgcg attcaagggt ctggccatcg
     2101 agcgcccgca gcgacttgta gacggcgctg atgacctcgg cgagctgtcc ggtcggcaag
     2161 gtattgttgc tgacataggc cgcgacgac
//
LOCUS       DASYNS010000460         3931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1988637, whole genome shotgun sequence.
ACCESSION   DASYNS010000460 DASYNS010000000
VERSION     DASYNS010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988637"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1514)
                     /gene="trmFO"
                     /locus_tag="VGP50_14420"
     CDS             complement(168..1514)
                     /gene="trmFO"
                     /locus_tag="VGP50_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV8028625.1"
                     /translation="MGSSIHIIGGGLAGSEAAWQAAREGAGVVLHEMRPVRATAAHVT
                     DSLAELVCSNSFRSDDAENNAVGLLHEEMRRAGSLILRAADAHRLPAGGALAVDRHGF
                     AAAVQQALLAEPSIELRREEVSGLPPAEWQSVIIATGPLTSPALAAAIRVESGADALA
                     FFDAIAPIVHRETIDMEKAWFQSRYGKGDGADYINCPLDKAQYESFVAALLAAEKTEF
                     HDWEKETPYFEGCLPIEVMAARGIDTLRYGPMKPVGLRDPRTGRGTYAVVQLRQDNAL
                     GTLYNIVGFQTKLKHGEQKRIFRMIPGLERAEFARLGGLHRNTFINSPKLLDGGLRLK
                     AMPRLRFAGQITGVEGYVESAAIGLLAGRFAAAELRGEMLSPPPETTALGALLRHITA
                     GADARTFQPMNINFGLFPPLPDGALTRANQGEKKPLIARRALADLDRWLGGRAAAA"
     gene            complement(1737..>3931)
                     /gene="uvrA"
                     /locus_tag="VGP50_14425"
     CDS             complement(1737..>3931)
                     /gene="uvrA"
                     /locus_tag="VGP50_14425"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV8028626.1"
                     /translation="ETALELADGLAIAENADTGEETTFSAKFACPVSGFTIPEIEPRL
                     FSFNNPFGACPTCDGLGTKLFFDPELVVPDEIKSLREGAVAPWAHSSSQYYAQTLDSL
                     ARHYKASTNEPWRDLPEKVRNAILFGSADTPVTMKYDDGVRAHTITRPFEGVIPNMER
                     RFAETDSAWLKEELGRFQNSRACEACHGKRLKPEALAVKIRGLDISVVADLSIADAAR
                     WFGELADHLTPKQRDIAQRVLKEINERLGFLGNVGLEYLTMSRGSGTLSGGESQRIRL
                     ASQIGSGLTGVLYVLDEPSIGLHQRDNERLLLTLKRLRDLGNTVIVVEHDEDAIRQAD
                     YLVDMGPAAGIHGGHVVAAGTPDEVLASAESITAQYLTGRKRIAVPETRRPGHPGQVL
                     RVVGARANNLKNITVDIPLGTFACVTGVSGGGKSTLVIDTLYKALARRLMGTREHPGA
                     HDAIEGAEHLDKIVDIDQSPIGRTPRSNPATYTGAFTPIRDWFSGLPEAKARGYGPGR
                     FSFNVKGGRCEACQGDGVIKIEMHFLPDVYVQCDVCKGKRYNRETLDITFKGESIADV
                     LDMTVDEGAEFFRAVPAIRDKMTTLQRVGLGYIHIGQAATTLSGGEAQRVKLAKELSR
                     RATGRTLYILDEPTTGLHFEDVRKLLDVLHALVEQGNTILVIEHNLEVIKTADWVVDL
                     GPEGGDGGGRVVATGTPEQIAAAPESYTGQYLRSYLARRDEKKKVRRA"
BASE COUNT          589 a         1319 c         1356 g          667 t
ORIGIN      
        1 ggtgaggggg cgacaccagt gagatgacgc gtgcaaaaac acacggccaa cgccaataaa
       61 atcgtggcgc ggccccctca ccctgaccct ctccccgagg ggagagggga tttgtgcata
      121 cgtgtcgcga tctgtgcaac tctgcttagg gcaagcaccg agtcggctca cgccgccgcg
      181 gcgcggcctc cgagccagcg gtcgagatcg gcgagcgcgc ggcgcgcgat gagcggcttc
      241 ttctcgcctt ggttggcgcg cgtcaaagcg ccgtcgggga gcggcgggaa gaggccgaaa
      301 ttgatgttca tcggctggaa tgtccgggca tccgcgccgg ctgtgatgtg gcgcagcaac
      361 gcgcccagcg ccgtcgtctc gggcggcggt gagagcatct cgccgcgcag ctccgcagcg
      421 gcgaagcggc cggcgagcaa gccgatggcg gcgctctcga catagccttc gacgcccgtg
      481 atctgtccgg cgaagcgcag ccgcggcatg gccttcaagc gcaggccgcc atcgagcagc
      541 ttcgggctgt tgatgaaggt gttgcggtga agcccgccca gtcgcgcgaa ctcggcgcgc
      601 tcgaggccgg ggatcatgcg gaagatgcgc ttctgctcgc cgtgtttgag cttcgtctgg
      661 aatcccacga tgttgtagag cgtgccgagc gcattgtcct gccggagctg caccacggca
      721 taagtgccgc ggcccgtgcg cggatcgcgc aagcccaccg gcttcatcgg cccgtagcgc
      781 agcgtgtcga tgccgcgcgc cgccatcact tcgatcggca ggcagccctc gaaataaggc
      841 gtctcctttt cccagtcgtg gaactcggtc ttctcggccg cgagcagcgc cgcgacgaac
      901 gactcatact gcgccttgtc gagcgggcag ttgatgtagt cggcaccgtc gcctttgccg
      961 tagcgcgact ggaaccacgc cttttccatg tcgatcgtct cgcgatggac gatgggcgcg
     1021 atggcgtcga agaaggcgag ggcgtcagcg ccgctctcca cccggattgc ggctgccagg
     1081 gccggggagg tgagggggcc cgtggcaatg atcacgctct gccattcggc cggcggcagg
     1141 cctgatacct cctcgcgccg gagctcgatc gacggctccg cgagcagcgc ctgctgcacc
     1201 gctgccgcga agccgtgccg gtcgacggcg agcgcgccgc ctgccggcaa tcggtgcgca
     1261 tccgctgcgc gcaggatgag cgagcccgcc cgccgcatct cctcatggag gaggccgacg
     1321 gcgttgttct cggcatcgtc ggagcggaaa gagttggagc agacgagctc cgcgaggctg
     1381 tcggtgacat gcgccgccgt ggcgcggacc ggccgcatct cgtgcagcac cacaccggcg
     1441 ccttcgcgcg ccgcctgcca cgccgcctcg cttccggcaa ggccgccgcc gatgatgtgg
     1501 atcgagctgc ccatgacgaa gagaatgagt aggggagttc gctgtgttgc gctacccccc
     1561 accctgaccc tccccctcaa ggggggaggg aaaagtcgga cctgcgctca ctcttccccc
     1621 tccccccttg agggggaggg cggggagggg ggtgtacgcg aacgcggctc ggcgcgggct
     1681 ctcgcggttg ctgacgcaac cgctggcgcc cgcctcagcc gacgcgccgc ctcggcttag
     1741 gcccgccgca ctttcttctt ctcgtcgcgg cgcgcgagat agctgcgcag atattgcccc
     1801 gtgtagctct cgggcgcggc ggcgatctgc tcgggcgtgc cggtggcgac gacccggccg
     1861 ccgccgtcgc cgccttcggg gccgagatcg acgacccagt cggcggtctt gatcacttcg
     1921 agattgtgct cgatcaccag gatcgtattg ccctgctcga ccagcgcatg gagcacgtcg
     1981 aggagcttgc gcacatcctc gaaatgcagc cccgtcgtcg gctcgtcgag gatatagagc
     2041 gtgcgccccg tggcgcggcg cgacagctcc ttcgccagct tgacgcgctg cgcctcgccg
     2101 cccgagagcg tcgtcgcggc ctggccgata tggatgtagc cgagccccac ccgctgcagc
     2161 gtcgtcatct tgtcgcggat cgccggcacg gcgcggaaga actcggcgcc ttcgtccacg
     2221 gtcatgtcga ggacgtcggc gatgctctcg cccttgaagg tgatgtcgag cgtctcgcgg
     2281 ttgtagcgct tgcccttgca gacgtcgcat tgcacataga cgtcgggcag gaagtgcatc
     2341 tcgatcttga tgacgccgtc gccctggcac gcctcgcagc gcccgccctt gacgttgaac
     2401 gagaaccggc cgggtccgta gccccgcgcc ttggcttcgg ggagacccga gaaccagtcg
     2461 cggatcggcg tgaaggcgcc ggtataggtc gcgggattgg agcgcggcgt gcggccgatg
     2521 ggcgactggt cgatatcgac gatcttgtcg agatgctcgg cgccctcgat cgcgtcatgg
     2581 gcgcccggat gctcgcgcgt gcccatgagc cgccgcgcca gtgccttgta gagcgtgtcg
     2641 atcacaagcg tcgacttgcc gccgccggac acgccggtga cgcaggcgaa ggtgccgagc
     2701 gggatgtcga cggtgatgtt cttgaggttg ttcgcgcgcg cgcccaccac gcgcagcacc
     2761 tggccgggat gacccggccg ccgcgtctcg ggcaccgcga tgcgctttcg tcccgtcagg
     2821 tattgcgcgg tgatgctctc ggcgctcgcc agcacttcat cgggcgtgcc ggccgccacc
     2881 acatggccgc catgaatgcc tgccgccggc cccatatcga cgagatagtc ggcctggcgg
     2941 atcgcatcct cgtcatgctc gacgacgatc accgtgttgc cgagatcgcg cagccgcttc
     3001 agcgtcagca gcagccgctc gttgtcgcgt tgatgcaagc cgatcgacgg ctcgtccagc
     3061 acatagagca cgccggtcag gcccgagccg atctgcgagg cgagccggat gcgctggctc
     3121 tcgccgcccg aaagggtccc ggagccgcgc gacatcgtca ggtattcgag accgacattg
     3181 ccgaggaagc ccagccgctc gttgatctcc ttgaggaccc gctgcgcgat gtcgcgctgc
     3241 ttcggcgtca agtgatccgc gagctcgccg aaccagcgcg ccgcgtcggc aatcgagaga
     3301 tccgcgacca cgctgatgtc gaggccgcgg atcttgacgg cgagcgcctc gggcttcaac
     3361 cgcttgccgt ggcaggcttc gcaagcccgc gaattctgga agcggcccag ctcctccttg
     3421 agccaggcgc tgtcggtttc tgcgaagcgc cgctccatgt tggggatgac gccttcgaag
     3481 gggcgcgtga tcgtatgcgc gcggacgccg tcgtcgtatt tcatcgtgac cggcgtgtcg
     3541 gccgagccga agaggatggc gttcctcact ttctccggca ggtcgcgcca cggctcgttg
     3601 gtgctggcct tgtagtggcg cgcgaggctg tcgagcgtct gcgcgtagta ttgcgagctc
     3661 gaatgcgccc agggcgccac cgcgccctcg cgcagcgact tgatttcgtc cggcaccacc
     3721 agctccggat cgaagaacaa cttggtgccg agcccgtcgc aggtcgggca ggcgccgaac
     3781 ggattgttga aggagaagag ccggggctcg atctcgggga tggtgaagcc cgagaccggg
     3841 caggcgaatt tcgccgagaa ggtcgtctcc tcgccggtgt cggcgttctc ggcgatggcg
     3901 agcccgtcgg cgagctcgag cgccgtctcc a
//
LOCUS       DASYNS010000461         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4795272, whole genome shotgun sequence.
ACCESSION   DASYNS010000461 DASYNS010000000
VERSION     DASYNS010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4795272"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2092)
                     /locus_tag="VGP50_14430"
     CDS             complement(<1..>2092)
                     /locus_tag="VGP50_14430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028627.1"
                     /translation="ALVDDSFDGVAVTDRGGGVIYANAAYLDLIGASDARDMRPVERV
                     FIGDADASEAIYRLLKAAREGKRLQEEVRVAGMHGRAARWLRLRVRPLAEGRRESRLT
                     VWSLTDVTRERERQENVFQELQHAIDYLDHAPAGFFSVDGKGDVVYLNATLANWLDQD
                     LAQVGSGGLKLTDLVAGDGAALLTTLRAAAGEIRTEVLDLDLKTRNGRTLPVRLFHRL
                     AFGADGRPGASRTLVLNRARDDGTDPQRAAEIRFMRFFHHTPMAIATVDRDGGIVRTN
                     SLFARLFHTVLSGEGDRSIGAVAAARDRVALDAAIAQAALGKGDIAPVDAALAGEPGN
                     ERFARFYVTAVEEAERDQETAIVYALETTAQRELETKVTQQQKMELVGQLAGGIAHDF
                     NNVLSAIMMAGDFLLNAHKPTDPSFQDIIQIKQNANRAASLVRQLLAFSRKQTLRPQV
                     LDVGEILGDLNMLLRRLIGEKVTLEVVHGRDLWPVKVDISQFEQVIVNLAVNARDAMA
                     DGGRLTLRTANVTAEECQRFHAKGMPAADYVLVEVADSGTGIPPAIVDKIFEPFFSTK
                     EVGRGTGLGLSTVYGIIKQTGGFIYVESAEQQGTTFRIFLPRHVAGAEEAAAERLKES
                     APAAVADVLAATEEVKRAASADLTGEGTILLVEDEEGLRALNARGLASRGYTVLEAGN
                     GIEAIEVLARSNGQV"
BASE COUNT          317 a          703 c          731 g          341 t
ORIGIN      
        1 gacctgtccg ttcgagcgcg ccaacacctc gatcgcctcg atgccgttgc cggcttcgag
       61 cacggtatag ccgcgcgagg cgaggccgcg ggcgttgagt gcgcgcaggc cctcttcgtc
      121 ctcgaccagg agaatcgtgc cctcgccggt gaggtcggcg cttgcggcgc gcttgacctc
      181 ctcggtcgcg gccaggacgt cggcgacggc cgctggcgcg ctttccttca gccgctctgc
      241 cgccgcctcc tcggcgccgg cgacgtggcg cggcaggaag atgcggaagg tcgtgccctg
      301 ctgctcggct gactccacat agatgaaacc gccggtctgc ttgatgatgc cgtagaccgt
      361 ggagagaccg agcccggtgc ccctgcccac ttccttcgtc gaaaagaatg gctcgaaaat
      421 cttgtcgacg atcgccggcg gaatgccggt gccgctatcg gccacctcta ccagcacgta
      481 atcggccgct ggcatgccct tggcgtggaa acgctggcat tcctcggcgg tcacattggc
      541 ggtccgcagc gtgagccggc cgccatcggc catggcgtcg cgggcattga ccgcgagatt
      601 cacgatcacc tgctcgaact gcgagatatc gactttgacc ggccagagat cgcgcccgtg
      661 taccacctcg agcgtgacct tctcgccgat caggcggcgc agcagcatgt tgaggtcgcc
      721 gaggatctcg ccgacgtcga gcacctgcgg acgcaaggtc tgcttgcgcg agaaggcaag
      781 gagctggcgc accaggctcg ccgcccggtt ggcgttctgc ttgatctgga tgatgtcctg
      841 gaatgacggg tcggtcggct tgtgggcgtt gagcagaaaa tcgcccgcca tcatgatggc
      901 cgagagcaca ttgttgaagt catgcgcgat gccgccggcg agctggccga cgagctccat
      961 cttctgctgc tgggtgacct tggtctccag ctcgcgctgc gcggtggtct cgagcgcgta
     1021 gacgatggcg gtttcctggt cgcgctccgc ctcttcgacc gcggtgacgt agaagcgcgc
     1081 gaagcgctcg ttgccgggct cgccggcaag cgccgcatcg accggggcga tgtcgccctt
     1141 gcccagcgcc gcctgcgcga tcgcggcatc gagcgccacc cggtcgcgcg cggcggcgac
     1201 ggcgccgatc gagcgatcgc cctccccgct gagcacggta tggaacagcc gcgcgaacag
     1261 cgaattggtg cgcacgatgc cgccgtcgcg atcgacggtg gcgatcgcca tcggcgtgtg
     1321 gtggaagaac cgcatgaagc ggatctcggc ggcgcgctgc ggatcggtgc catcgtcgcg
     1381 ggcgcgattg agcaccaggg tgcgcgaggc gccgggccgg ccgtcggcgc cgaaggcgag
     1441 ccggtggaac aggcgaaccg gcagcgtgcg gccgttgcgg gtcttcagat cgaggtcgag
     1501 cacctcggtc ctgatctcgc cggctgcggc gcgcagcgtg gtcaacagcg ctgcgccgtc
     1561 gccggcgacg agatcggtca gcttcagacc gccggagccg acctgggcga ggtcctgatc
     1621 gagccaattg gcgagcgtgg cgttgaggta gacgacgtcg cccttgccgt cgacggagaa
     1681 gaatccggcc ggcgcgtgat cgagatagtc gatcgcgtgc tgcaattcct gaaagacatt
     1741 ctcctgccgc tcgcgctcgc gggtaacgtc ggtgagcgac cagaccgtga ggcggctctc
     1801 gcgccggccc tcggcgagcg gccgcacccg cagccgcagc cagcgcgcag cccggccatg
     1861 catcccggcg acgcgtacct cctcctggag gcgcttgcct tcgcgcgccg ccttgaggag
     1921 ccggtagatc gcttcggacg cgtcggcgtc gccgatgaac acgcgctcca ccgggcgcat
     1981 atcccgcgca tcagaggcgc cgatcaggtc gaggtaggcg gcattggcgt agatgacgcc
     2041 gccgccgcga tcggtgacgg caacgccgtc gaacgaatcg tcgaccagcg cc
//
LOCUS       DASYNS010000462         4470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4488622, whole genome shotgun sequence.
ACCESSION   DASYNS010000462 DASYNS010000000
VERSION     DASYNS010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4488622"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VGP50_14435"
     CDS             <1..542
                     /locus_tag="VGP50_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028628.1"
                     /translation="ETVSRLSEMAGSWEANVVLTGGGGGCPAPIAVPGFLSPMPIICT
                     SGSNGLVALAPILSAPTVSIPVSVSAVAALPAPIAALPALRAVAPALAAAPATIEAAS
                     PTPGTVVLTFTPTTRPPPPPTARVTPPPPFRPAPAPTRSNSTPPPQTPPHTERWTGLP
                     THVTINGPINGKIDITRHQ"
     gene            558..1787
                     /locus_tag="VGP50_14440"
     CDS             558..1787
                     /locus_tag="VGP50_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028629.1"
                     /translation="MRYTPSSPKERWSGLRDWSRRRRFIVAAFALSALILILLLTAPA
                     RHDGEFTAAFYRETADLVRSGLGLVLLAPLLIAFSLLLLLIVYFWLSNLFSDGRGVDR
                     ALEKLLPYFGRAVLLLVPLAMVLVIGFGALRAYVTPEVSEHKIARVAALHATTSPLLL
                     NLGTHALTAPEARLSGTFLALGDNVISIAAFPPLPLFKEPVEPISDLLSRGDDLLVIT
                     GRKLFQIDRAGNVGLLARLPLDAMRLGAFAEPRDVSAKILLTGNKENAGYVFELTSEQ
                     EYSKLAELDAPVIGAAGCLDDVAIAVADRVLLVSPGRLPRLLFRRPQNDPPFTSIRAR
                     WVEGMTPPCLWLFATQNQIYLHQDGLNLLLATGLGGLITDTSLDAIPDGAPSLRFTLL
                     DAPRRTIVDLTLDRRPQ"
     gene            1914..2579
                     /locus_tag="VGP50_14445"
     CDS             1914..2579
                     /locus_tag="VGP50_14445"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028630.1"
                     /translation="MPDTALITGFEPYGGRAVNPSGRLALALDGARVGGFQIVGRTLP
                     VVLAGLGERLEAYLEEARPALVIALGLWPGEPMLRLERLAVNLADFAIADNAGVRLED
                     AAIVPDGAAALLATLPLRAIESALLADGIPARLSTTAGTYLCNATLYTLLRLVEQRGR
                     RIPCGFIHVPYLPEQVAETLQQARAGRVDLHQRADIASMDFAVMERALRLALIVSGQS
                     SAR"
     gene            2808..3908
                     /locus_tag="VGP50_14450"
     CDS             2808..3908
                     /locus_tag="VGP50_14450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEV8028631.1"
                     /translation="MRNRLFTSLSLMGLAAGLWLAPGGASAQEYVQTNLVSDLATVGA
                     VTVDPNLKNAWGIARGAATPWWVNAEGTGTSRLYTGAGQVVAALPFVTIPTPSGTGIS
                     RPTGIIFNGSTDFDLVAGNPATSAIFLFDTEDGTIAGWNPGVNPTVAVTKVNESAQHA
                     LFTGLTWIVVNNEHFLLAANFFAGTVEAFDANFNRVQDFGFNPFPGFAPYNVQAVGAN
                     VVVTYAQQNATKTGPVNGTGVGFVILFNDQGVPVQVLEEGPWFDSPWGAELAPQDFGT
                     FSHALLIANRGSGTIAAFNAADGQFLGNFEDTSGNTLVIPGLWGIETASGSAASGSAT
                     ALYFAAGINGYVDGLFGTLTPVAAELNVEDHE"
     gene            complement(4025..>4470)
                     /locus_tag="VGP50_14455"
     CDS             complement(4025..>4470)
                     /locus_tag="VGP50_14455"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HEV8028632.1"
                     /translation="MPAGLGFHPSFPRRPAAVVSAATSGVWLADAECLPTEKTTVPAA
                     WDLAEGRALGDLALDHVFTGWSGRAVIAWPDQGASLALEACHPLLSFLAVATRPEAES
                     FCLEPVSHCADAINLARHGVADTGLRVLAPGVRRSAKLTLRPRWV"
BASE COUNT          688 a         1606 c         1374 g          802 t
ORIGIN      
        1 aggaaaccgt atcgcggctg agcgagatgg ctggcagttg ggaggccaat gtcgttctca
       61 ccggcggcgg gggcggatgt ccggcaccca tcgccgtccc cggcttcctc tcgcccatgc
      121 ccatcatctg caccagcggc agcaatggtc tggtggcgct agcgcccatt ctttcggcac
      181 cgacggtcag catcccggtt tcggtcagcg ccgtcgccgc cttgcccgcg ccgatcgcag
      241 cgttgcccgc cttgcgcgcc gtcgcgcccg cgctcgccgc cgcccctgcg accattgaag
      301 ccgccagccc cacgcccggc accgtggtcc tcaccttcac gcccaccacc cgcccgccgc
      361 cgccgccaac cgcccgcgtc acaccgcccc cgccctttcg cccggcaccg gcgccgaccc
      421 gcagcaacag cacgccgccg ccgcaaactc cgcctcatac cgagcgttgg accggcctgc
      481 cgacacatgt gacgatcaac gggccgatca acggcaagat cgacataacg cgccatcaat
      541 aacgaacgac catcctcatg cggtacactc cctcttcgcc gaaagagcgc tggtccgggt
      601 tgcgggactg gagccggcgg cgccggttca ttgtcgcggc gttcgctctt tcggcgctta
      661 ttctcatcct gctgctcacc gctcccgcgc ggcatgacgg cgaattcacc gccgcctttt
      721 atcgcgagac cgccgatctg gtgcgttccg gccttgggtt ggtgttgctg gcaccgctgc
      781 tgattgcctt ttctttgctc cttctcctca tcgtttattt ttggctttcc aacctgttct
      841 ccgatggccg cggcgtcgat cgcgccttgg agaagctgtt gccttatttc ggtcgcgcgg
      901 tcttgctgct ggtcccgttg gccatggttt tggtcattgg cttcggcgcc ttgcgcgctt
      961 atgtgacacc tgaagtgagc gagcataaaa tcgcccgtgt cgcggcgctc cacgccacca
     1021 cctcgccctt actgctcaat ctcggcactc atgccttgac cgcgcccgag gcgcggctga
     1081 gcggcacgtt tctggcgctg ggcgacaatg tcatttccat cgcggcgttt cccccgctgc
     1141 cgcttttcaa ggagccggtc gagcccatct ccgatctcct gagccgcggc gacgacctgt
     1201 tggtgatcac cggccgcaaa ctttttcaaa tcgaccgcgc cggcaatgtt ggtcttctgg
     1261 cgcgcctgcc gctcgacgcg atgcgccttg gcgctttcgc cgaaccgcgc gatgtcagcg
     1321 ccaagatctt gctcaccggc aacaaggaga atgcaggcta tgtcttcgag ctcaccagcg
     1381 agcaggaata tagcaagctc gccgagttgg acgcgccggt gattggtgcc gccggttgcc
     1441 tcgacgatgt ggccatcgcg gttgccgatc gcgttctcct ggtctcgccc ggccgcctgc
     1501 cgcgcttgct gtttcgccgg ccgcaaaacg acccgccctt cacctcgatc cgcgcccgct
     1561 gggtcgaggg catgacgccg ccctgcctgt ggctcttcgc cacgcaaaat cagatctatc
     1621 ttcaccaaga cggattgaat ctattgctcg cgacgggtct cggcggcttg atcacggata
     1681 cgagcctcga cgccatcccc gacggcgcgc cgtccttgcg ctttacgctg ctcgacgcgc
     1741 cgcgccgcac catcgtggat ctgacgctcg accgccgacc gcaataacca gtgccggcgc
     1801 ggccaagaat tcgccatggc gacgcaccgc cgcaacgtcg cggcgagttg cgccgccgcg
     1861 aagacatgcg tcataaggcg tcagcgacac atcgtcgcgc gaggctccgc gtcatgcccg
     1921 acacggcact gatcaccggt ttcgagccct atggtggccg cgcggtcaat ccgtcgggac
     1981 gtctggcgct ggccctcgac ggcgcgcgcg tcggcgggtt ccagatcgtc ggccgcacgc
     2041 tgcccgtggt ccttgccggc ctcggcgagc gcctcgaagc ctatctcgaa gaggcgcgcc
     2101 ctgccctcgt gatcgcgctc ggcctctggc cgggcgagcc gatgctgcgg ctcgagcgcc
     2161 tcgccgtgaa ccttgccgat ttcgccatcg ccgacaatgc cggagtgcgg ctcgaggatg
     2221 cggcgatcgt ccccgacggc gccgcggcgc tcttggcgac gctgccgctc cgcgccatcg
     2281 agagcgcgct cctggccgac ggcatcccgg cgcggctctc gaccaccgcg ggcacctatc
     2341 tctgcaacgc gacgctctac acgcttttgc ggctcgtcga gcagcgtggt cgccgcattc
     2401 cctgcggctt catccatgtg ccttatctgc cggagcaagt ggccgagacc ctgcagcagg
     2461 cgcgcgcagg ccgcgtcgac ctccatcaac gtgcggacat cgcctccatg gacttcgccg
     2521 tcatggagcg cgccttgcgc ctggccctga tcgtctccgg ccagtcgagc gcgcgctgag
     2581 cgccgcctcg gcgcgacaaa gttcgagttc ctgacaatct gtcgtcaaac aactggatca
     2641 tctgcgatct cgcctggcgg gggtattgaa gtcgtccgat cacgccttcg tggttggacc
     2701 catgcaaagg catatcgata atttcgcccg tcgcaacaat tcttgcgccg atagcatctt
     2761 ggcgagcgcg tggctgaacc aatcaacgag atccggggag catccgcatg cgtaacagat
     2821 tgttcacgtc attgtctttg atgggactgg ctgccgggct gtggctcgcg ccgggcggcg
     2881 cgtccgcgca ggaatacgtc cagacgaacc tcgtttccga cctcgccacc gtgggcgcgg
     2941 tgacggtcga tccgaacctc aagaacgcat ggggcatcgc ccgcggcgca gccacgccgt
     3001 ggtgggtcaa cgccgaaggg accggcacgt ctcgcctcta taccggcgcg ggccaggtcg
     3061 tcgccgcgct gcctttcgtg acgatcccga cgccgagcgg cacgggcatc tcgcggccga
     3121 cgggcatcat cttcaacggc agcacggatt tcgatctcgt cgccggcaac ccggcgacgt
     3181 ccgccatctt cctcttcgac accgaggacg gcaccatcgc cggttggaac ccgggcgtga
     3241 atccgacggt cgccgtcacc aaggtcaacg agtccgccca gcacgccttg ttcaccggcc
     3301 tgacctggat cgtcgtcaac aacgagcatt tcctgctcgc ggcgaatttc ttcgccggca
     3361 ccgtcgaggc cttcgacgcg aacttcaatc gtgtccagga tttcggcttc aacccctttc
     3421 cggggttcgc gccctacaac gtgcaagccg tcggcgcgaa tgtcgtcgtc acctacgccc
     3481 agcagaatgc gaccaaaacc ggcccggtca atggcaccgg ggtcggcttc gtcatcctct
     3541 tcaatgatca aggcgtgccc gtgcaagtgc tggaggaggg cccgtggttc gattcgccct
     3601 ggggtgccga gctggcgccg caggatttcg gcaccttcag ccatgccctg ctgatcgcca
     3661 accgcggcag cggcacgatt gccgccttca acgcggcgga cggacaattc ctgggcaact
     3721 tcgaagacac gtcgggcaac acgctcgtca tccccggcct ctggggcatc gagacagcga
     3781 gcggctcggc ggccagcggt tccgcgaccg ccctctactt tgccgccggc atcaacggct
     3841 atgtcgacgg gttgttcggc acgctgaccc cggttgccgc cgagctcaac gtcgaggatc
     3901 acgagtagca cgcacgcgcc gccgtcgcgg tgacggcatc aacgcgcgag tttgacgaaa
     3961 ggaagggaac gcgtcggctc cgtgccggcg cgttctcgca accagatcaa atcgcggatg
     4021 ggatctaaac ccagcgcggc cgaagcgtca gcttggcgct gcggcgcact cccggcgcga
     4081 ggacgcgcag cccggtatcc gcgacgccgt ggcgcgcgag attgatcgcg tcggcgcaat
     4141 ggctcaccgg ctcgaggcag aaggactccg cctcgggccg cgtcgccacg gcgaggaagg
     4201 agaggagcgg atggcaggcc tcgagcgcga ggctcgcgcc ctgatccggc caggcgatca
     4261 cggcgcggcc gctccagccg gtgaagacgt ggtcgagagc tagatcgccg agcgcgcggc
     4321 cttccgccag gtcccaggcc gccggcaccg tcgtcttctc ggtcggcaga cactcggcgt
     4381 cggcgagcca cacgccgctc gtggcggcgc tcaccacggc agcaggccgg cgcggaaagc
     4441 tcggatggaa accgagccct gccggcatca
//
LOCUS       DASYNS010000463         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1521845, whole genome shotgun sequence.
ACCESSION   DASYNS010000463 DASYNS010000000
VERSION     DASYNS010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521845"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /gene="boxB"
                     /locus_tag="VGP50_14460"
     CDS             <1..1049
                     /gene="boxB"
                     /locus_tag="VGP50_14460"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEV8028633.1"
                     /translation="QQRLLGHTAPSLYDLRNLFQVNVEEGRHLWAMVYLLHAYFGRDG
                     REEAEELLTRHSGDADKPRILGTFNEPISDWLSFFMFTYFTDRDGKFQLKSLAESGFD
                     PLARSCQFMLTEEAHHMFVGDTGIARVVRRTLEVMKELGSEDPAAVRAKGAVDLPTIQ
                     RYLNFWFSSSLDLFGAEISSNAASYFATGIKGRPDEANFADHLCKEGAMALDVPDGKG
                     GVTCDAVPLRNAMNEAVRSAYVRDCEIGVKRWNRLIVKAGYSLELTLPSPRFRRGIGA
                     WAGQPVDPAGHVIGAAEWQAKLPGWIPSDADRAFVKNLMQRVTEPGKMAAWIAPPERG
                     INNLPVEYEYVHLH"
     gene            1235..2476
                     /locus_tag="VGP50_14465"
     CDS             1235..2476
                     /locus_tag="VGP50_14465"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV8028634.1"
                     /translation="MSPRTVPRFVIIGAGPAGMRAAEVLRSQSAEAEIVLIGDEPYPP
                     YDRPPLSKAFITNGVAPASLSLKPESFYTEQRIELKLGRAALAIDRGAKRVALAGGEH
                     VAYDKLLLATGCGSRRLPPSLAPPSVHYLRSLEDAARIRAALAQGTSLVLIGGGFIGL
                     ELAASATTLGVEVTVLEAMPRLLTRGLPAPVCDFMHRLHERHGVRFELDARLKSIAGE
                     EGDFVVSTERATYRCGIVVAGIGAVPHTRLAEAAGLAVEDGIRVDASGRTGDADIFAA
                     GDATRHDNPLLGRAIRVESWQVALNQAAVVARAMLGGNDVYAEMPWLWTDQYDCNIQV
                     LGCFDPSLELLLRGDVRSGAFTLLGLGADGRITLAVTVNNGRDMAVLRRLVTSKASPP
                     KDALADPTRKLADLLKAARAS"
     gene            complement(2654..3115)
                     /locus_tag="VGP50_14470"
     CDS             complement(2654..3115)
                     /locus_tag="VGP50_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV8028635.1"
                     /translation="MNETIDWRSHGVRVVKGSALDTNTPQTAGMNRAAAINYATAGAQ
                     KLWAGTVTIHANAKTGAHHHGALESVIYVVKGKARMRWGETLEYMAEAGPGDFIYVPP
                     YVPHQEINASQDEPLECVLVRSGQEPVVVNLDIAPAEPPEEVRWIDPIHKS"
     gene            complement(3126..>3357)
                     /locus_tag="VGP50_14475"
     CDS             complement(3126..>3357)
                     /locus_tag="VGP50_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV8028636.1"
                     /translation="RDRFLRNVLIAIGNSQEPRLIAAVKPLLPDKSPLVRAMAVWALA
                     RLGDRNAVDALREAHLPKEEDEAVRAEWRRAA"
BASE COUNT          511 a         1141 c         1127 g          578 t
ORIGIN      
        1 agcagcaacg gttgttgggc cacaccgcgc cgtcgctcta tgatttgcgc aacctcttcc
       61 aggtgaatgt cgaggagggg cggcatcttt gggcgatggt ctatctgctc catgcctatt
      121 tcggccgcga cgggcgcgag gaagcggagg agctgctgac gcgccattcg ggcgatgccg
      181 acaagccgcg catcctcggc accttcaacg agccgatcag cgactggctc tccttcttca
      241 tgttcaccta tttcaccgac cgcgacggca agttccagct gaagagcctc gccgagagcg
      301 gcttcgatcc gctcgcgcgc agctgccagt tcatgctgac cgaggaagcg catcacatgt
      361 tcgtcggcga taccggcatc gcccgcgtcg tgcgccgcac gctcgaagtg atgaaggagc
      421 tcggcagcga ggatccggcg gcggtgcggg ccaagggcgc ggtcgatctt ccgaccattc
      481 agcgctatct caatttctgg ttcagctcct cgctcgacct cttcggcgcg gagatctcct
      541 cgaacgccgc cagctatttc gcgacgggca tcaagggccg gccggacgaa gcgaatttcg
      601 ccgaccatct ctgcaaggaa ggcgcgatgg cgctcgacgt gcccgacggc aagggcggtg
      661 tcacttgcga cgccgtgccg ctgcgcaatg ccatgaacga agcggtgcgc agcgcttatg
      721 tccgcgactg cgagatcggc gtcaagcgct ggaaccgcct catcgtcaag gcgggctaca
      781 gcttggaatt gacgctgccg agcccgcgct tccgccgtgg catcggcgcc tgggcgggcc
      841 agcccgtcga cccggcgggc catgtcatcg gcgccgcgga atggcaggcg aagcttccgg
      901 gctggattcc gtcggacgcc gatcgcgcct tcgtcaaaaa cctgatgcag cgcgtgaccg
      961 agcccggcaa gatggctgcg tggatcgcgc cccccgagcg cggcatcaat aacctgccgg
     1021 tcgagtacga atacgtccat ctgcattgag acttgaggac gcgcaagcgc gcttctccct
     1081 ctccccttgc gggagaggga ggggcccatg cgaagcatgg gagggtgagg ggtgaagcgg
     1141 cacatcatct cagaatgcgc agccgggctt cactcctcac ccggcgacgc tgcgcgtcgc
     1201 caccctctcc cgcaagggga gagggttggt ctgcgtgtcg cctagaactg tgccacgctt
     1261 cgtcatcatc ggcgcagggc cggcggggat gcgcgcggct gaggtgctgc gcagccaatc
     1321 ggccgaggcc gagatcgtcc tgatcggcga cgagccctat ccgccctatg accggccgcc
     1381 gctgtcgaag gcgttcatca ccaacggcgt cgcgccggca agcctctccc tgaaacctga
     1441 gagcttctac accgagcagc ggatcgagct gaagctcggc cgcgccgcgc tcgccatcga
     1501 ccgcggggca aagcgcgtcg ctctcgcggg cggcgaacat gttgcttacg acaagctgct
     1561 gctggcgacg ggctgcggca gccggcgatt gccgccgtcc ctggcgccgc cttcggtgca
     1621 ctacctgcgc agcctcgagg acgcggcgcg gatcagggcg gcgctcgccc agggcacatc
     1681 gctcgtcctc atcggcggcg gcttcatcgg cctcgagctc gcggcgtcgg ccaccacgct
     1741 cggcgtcgaa gtgacggtgc tcgaagcgat gccgcgcctt ctgacgcgcg ggctcccggc
     1801 gccggtctgc gatttcatgc atcgcctgca tgagcgccat ggcgtgcgtt tcgaactcga
     1861 tgcgcggctc aagagcatcg ccggcgaaga gggtgatttc gtcgtgagca cagagcgtgc
     1921 gacctatcgc tgcggcatcg tcgtcgcggg catcggcgcc gttccccata cgcgcctcgc
     1981 cgaggcggcg ggccttgccg tcgaggacgg cattcgcgtc gatgcgagtg gccgcaccgg
     2041 cgatgccgac atcttcgcgg ccggcgacgc gacgcggcac gacaatccgc tgctgggccg
     2101 cgccattcgc gtcgaatcct ggcaggtcgc gctcaaccag gccgccgtgg tcgcgcgcgc
     2161 catgcttggc ggcaacgacg tctatgccga gatgccctgg ctctggaccg accaatatga
     2221 ttgcaacatc caggtgctcg gctgcttcga tccgagcctc gagctgctgc tgcgcggcga
     2281 cgtccgttcg ggcgcgttca cgctgctcgg cttgggcgcg gatgggcgaa tcaccctcgc
     2341 ggtcacggtg aacaacggcc gcgacatggc cgtgctgcgc cgcctggtga cgtcgaaggc
     2401 gagtccgccg aaagacgcgt tggcggatcc gacccgcaag ctcgcggatc tcctgaaagc
     2461 cgcgcgcgcg agctgagcat tccctctccg cgcgaagcgg ggagagggaa ggggcccgtc
     2521 gcgtgagcga cgggaagggt gaggtgtctc tccatgacac cagcgagtcc ggcgtcgaca
     2581 cccacctcac ccgctcgctg cgctcgcgcc ctctcccccc gcaagagcgg gcggagaggg
     2641 cattgttcag cgatcagctc ttgtggatcg gatcgatcca gcgcacttct tccggcggct
     2701 cggccggcgc gatgtcgaga ttgacgacga ccggctcctg gccgctgcgc accagcacgc
     2761 attcgagcgg ctcgtcctgg ctcgcattga tctcctgatg cggcacatat ggcggcacat
     2821 agatgaagtc gccgggtccc gcttcggcca tgtattcgag cgtctcgccc cagcgcatgc
     2881 gcgctttgcc cttcaccaca tagatcacgc tctcgagcgc gccatgatga tgggcgccgg
     2941 tcttggcgtt ggcatggatc gtcaccgtgc ccgcccagag cttttgcgcg ccggccgtcg
     3001 catagttgat ggccgcggcc cggttcatgc cggccgtctg cggcgtgttg gtgtcgagcg
     3061 cgctgccttt gacgacgcgc acgccatggc tgcgccagtc gatcgtctcg ttcatgggtg
     3121 cctcctcatg ccgcccggcg ccattccgcg cgcacggctt cgtcctcttc cttcggcaag
     3181 tgcgcttcgc gcagggcgtc gacagcgttg cggtcgccga ggcgcgcgag cgcccacacg
     3241 gccatggcgc gcaccagcgg cgacttgtcc gggagcagcg gcttcaccgc cgcgatcagg
     3301 cgcggctctt ggctgttgcc gatggcgatg aggacgttgc gcaggaagcg gtcgcgg
//
LOCUS       DASYNS010000464         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3549879, whole genome shotgun sequence.
ACCESSION   DASYNS010000464 DASYNS010000000
VERSION     DASYNS010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3549879"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VGP50_14480"
     CDS             complement(<1..733)
                     /locus_tag="VGP50_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028637.1"
                     /translation="MTTRRHALKLLAAGAFSVLGLPGVVFAQAPGDRRFVLVLLRGAL
                     DGLAAVPPIGDPHYAGRRGAVALKPPGEPGGCLALDKLFALNPALAAIHPYWGKGELL
                     FVHASGNGYRTRSHFDAQDLMESGLSAKGTMSDGWLNRALAQLQKGDHRLGLAVGGAV
                     PLVLRGPVPVASWEPAALQDTSPEFMTALAQLYRGDPLLGPALGEGLKGQNFSESVLG
                     DDMAGGKRGFGPKSFAPLAAAAGKLL"
     gene            complement(738..2066)
                     /locus_tag="VGP50_14485"
     CDS             complement(738..2066)
                     /locus_tag="VGP50_14485"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEV8028638.1"
                     /translation="MQASGDAVNAATRFGFAARGGDLALIAKDPRGWVLGQLAQQPAA
                     LPGDLPSAQTMVVAEFEMRRDKGNEDAKRAFEERAKSVYFAEIAARIRAAVASESPLL
                     ERLTQFWSNHFTVSVLRPVLRGFAAAFEREAIRPHVTGRFSDMLLAVARHPAMLLYLD
                     NAISVGPNSIAGRERDKGLNENLGRELLELHTLGVDGGYTQNDVEALARILTGWSVAR
                     LNEPDPGAFRFRPRIHEPGPKRLLGKIYNEAGYAEGEAALLDLAHHPATARHVATQLA
                     RHFIVDDPPKDSVERISRVFRDSGGDLRRVTAAVVKEDAAWRAPLAKIRRPEELVIAA
                     CRVSGFTPPPEMLVNSLRSLDQMPFFAPSPAGWPDIAAAWVSPEAVLRRAQWCERYAD
                     RIPDPPDPVAIAEQAFGEALPAETADAISLAPSRRAGLALLLASPQFQRR"
BASE COUNT          373 a          795 c          817 g          350 t
ORIGIN      
        1 cgaggagctt gccggcggcc gcggcgagcg gcgcgaaact cttggggccg aagccgcgct
       61 tgccgcccgc catgtcgtcg ccgagcacgg attcggaaaa attctgcccc ttcaatccct
      121 cgccgagcgc tggcccgagc agcggatcgc cgcgatagag ctgagccagc gccgtcatga
      181 attcgggcga ggtgtcctgg agcgccgccg gctcccagga tgcgaccggc accggcccgc
      241 gcagcacgag cggcacggcg ccgccgactg cgagtccgag gcgatggtcg cccttctgca
      301 gctgcgccag cgcgcggttg agccagccgt cgctcatggt gcctttggcc gagagcccgc
      361 tctccatcag atcctgcgcg tcgaaatgcg agcgcgtgcg gtagccattg ccggaggcat
      421 ggacgaaaag cagctcgcct ttgccccagt aagggtggat cgccgccagc gccgggttca
      481 gcgcgaagag cttgtcgagc gcgagacagc cgccgggctc gccgggcggc ttcaaggcga
      541 cggcgccgcg gcggccggca taatgcggat cgccgatcgg cggcacggcg gcaaggccgt
      601 cgagcgcgcc gcgcagcagc accaggacga agcggcggtc gcccggcgcc tgagcgaaga
      661 cgacgccagg caagcccagc accgagaagg caccggcggc caacagcttc agagcatggc
      721 ggcgcgtggt catggcttca ccgtcgttgg aattgcgggc tcgccagcag cagcgcgagg
      781 ccggcgcgcc gcgagggcgc gaggctgatg gcgtccgccg tctcggccgg cagcgcctcg
      841 ccgaaggctt gctccgctat ggcaacaggg tcgggcggat cgggaatgcg atcggcgtag
      901 cgctcgcacc attgcgcgcg gcgcagcacg gcctcgggcg acacccaggc cgcggcgata
      961 tcgggccagc ccgcgggcga tggcgcgaaa aacggcatct ggtcgagcga gcgaaggctg
     1021 ttgacgagca tctcgggggg cggcgtgaat ccgctgacgc gacaggccgc aatcaccagt
     1081 tcctcgggcc ggcggatctt cgcgaggggc gcgcgccacg ctgcgtcctc cttcaccacc
     1141 gccgccgtca cccggcgcag gtcgccgccg ctgtcgcgga acacccgcga gatgcgctcg
     1201 acgctgtcct tgggcggatc gtcgacgatg aaatggcggg cgagctgcgt cgccacatgg
     1261 cgtgccgtcg ccggatgatg ggcgagatcg aggagcgccg cctcgccttc ggcatatccg
     1321 gcttcgttgt agatcttgcc gagcagccgc ttcgggccgg gctcatggat ccgcggacgg
     1381 aagcggaagg cgccgggatc gggctcgttg agccgcgcca ccgaccatcc ggtgaggatg
     1441 cgcgccagcg cttcgacatc gttttgcgtg tagccgccgt cgacgccgag cgtgtgcagc
     1501 tccagcagct cgcgtccgag attctcgttg aggcccttgt cgcgctcgcg tccggcgatc
     1561 gagttgggtc cgaccgaaat ggcattgtcg agatagagca gcatcgccgg gtggcgcgcc
     1621 accgccagca gcatgtcgct gaaccgtccc gtcacatgcg ggcggatcgc ctcgcgctcg
     1681 aaagcggcgg cgaagccgcg cagcaccggc cgcagcaccg acacggtgaa atggttgctc
     1741 cagaattgcg tgagccgctc gaggagcggc gactcgctgg cgacggcggc gcggatacgc
     1801 gctgcgatct cggcgaaata aacggacttc gctcgttcct cgaaggcgcg ttttgcgtct
     1861 tcattgccct tgtcgcggcg catctcgaac tcggccacga ccatggtctg agcactcggc
     1921 agatcgcccg gcagcgcggc cggttgctgc gcgagctggc ccagcaccca gcccctgggg
     1981 tccttggcga tgagggccag atcaccgccg cgcgccgcaa aaccgaagcg cgtcgccgca
     2041 ttcacggcat cgccgctcgc ttgcatttta acgatccccg gatgatgggc gcatcaccgg
     2101 atagtacgtc cgaaagggtt cagatatccc tgccggagat accgccgtgg tgaccacccc
     2161 tgtcggtccg acccgcaatg gcgggagagg ccgtgctaaa tcgggctgcc ctggagctcc
     2221 ttgcgcagtt cctcgacgtt ctcgcggaca ccgctcaaat gcgggtcgat cttcagcgcc
     2281 gcttcgaaag ccttgagcgc cagcgccttc ttctccatcg cgagatagat ctggc
//
LOCUS       DASYNS010000465         4108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_275655, whole genome shotgun sequence.
ACCESSION   DASYNS010000465 DASYNS010000000
VERSION     DASYNS010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275655"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VGP50_14490"
     CDS             complement(<1..203)
                     /locus_tag="VGP50_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEV8028639.1"
                     /translation="MTLAGGNLFADLPARLAEERFDELLGAPDLRIERIVSTGHTTPP
                     GVFLDQEWAEWVVLLQGAATLLF"
     gene            359..979
                     /locus_tag="VGP50_14495"
     CDS             359..979
                     /locus_tag="VGP50_14495"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8028640.1"
                     /translation="MSSRERILEAAMTVFARFGYRRASMDQIAEEVALTRQAVYHHFP
                     GKEALFRAVVEDLHEGAYEAEAAAGRQEEQAGRSLADILAAQIDARFRYIIDCVETSP
                     YAEELLSEHQLQTRDLYESFIEHNIGLRVETIDRVCAAQGLALRPDMTSLDLARCIQI
                     AIRGFSATQTNADVLADLDRMVRLLVAGAVAPGAARTARASKRSLR"
     gene            976..3663
                     /locus_tag="VGP50_14500"
     CDS             976..3663
                     /locus_tag="VGP50_14500"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV8028641.1"
                     /translation="MSSVTVNGRRQTLPDDADALLVDVLRHDLGLTGTKLVCAAGVCG
                     ACTVLLDGAPVASCLLPASAAAGKTVTTVEGIGTAKLHAVQKAFMALDALQCGFCTPG
                     FVVEAVAFYEHWRKTRSGATPTREEIGAALSGHLCRCGAYDNILRAVAAACAGRFEGD
                     DVTSPRIEAREKVTGAAKYTVDIQHEGQLEGVVLRSAVAHARIAALDLAPALAVPGVK
                     AAISLLDEDRTVRYVGEPIAAVAASDRKTAKRAAAAIKIVYEPLPSVIGLDAARKPDA
                     AVVFGKSRKRAGNVSEGTAMPTPWKGNVRGPSAPFSHGKSNARKWLAAARAEHDPLLV
                     EATFRTGAQSHTCLEPHAAVARFDGERLTVHVSTQAVFHLMELIAKRFKLAPDKVKVI
                     AEHVGGGFGSKATLGMETIVAIELARKAKAPVRVVFDRHEELSVTGYRPGAELNIALL
                     PSSRGHLRALTLSAYADTGAAVNSTIAALARLIYPAEAKELADFDVVSNLPPGAPFRG
                     PGGPPMAFALEQAVDEAALRLKIDPIALRRRWDHNPNRRRLYDWAAGLDTWRRAPAAG
                     AQTGRYRRGVGVAAGYWLHLWQPGSKVELAVKSGRIVASTAVQDMGTGIRSVIADTIA
                     REFGLEPQEIEVRIGDSSLPEGPASGGSRTTASIIPPALLAVRKLKTAIEHSSKGAPM
                     PGSNAPWRAVIAATPDVQVAAERPEDDRRTAAGMHSPLKEVGFIGTVFGFMMRRFSNI
                     VIGAGVPSSVQVIEVEVDTLLGRVRVLTAHTGIAVGEIAAPALAKSQAAGSVIQGIGY
                     ALYEGREVDPQSGDILTAGLEDYRIPGIADTPVIDVHLDEGGFGHVLGGSAGLGEVAT
                     VPTAAALANAIRHAVGVRPQEIPLRPDRLLGLLNARRSA"
     gene            3660..>4108
                     /locus_tag="VGP50_14505"
     CDS             3660..>4108
                     /locus_tag="VGP50_14505"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV8028642.1"
                     /translation="MSATDAEFRAAGTDLSERRRSGLSRGTLIDLAASAEMTGIEWGA
                     DGAARIGALTRIAAIAADLRMAMAYPGLVAAAGGLATPQIRHLATLGGNLAQRSRCWD
                     YRNPQIACLKKGGATCPARDGNHLYGVAFDLGPCVAPHPSTMAAALLA"
BASE COUNT          650 a         1413 c         1405 g          640 t
ORIGIN      
        1 tcgaagagca gcgtggccgc gccctggaga aggacgaccc actccgccca ttcctgatcc
       61 aagaagactc cgggcggcgt cgtgtggccg gtcgagacga tgcgctcgat gcggagatcg
      121 ggcgccccga gcaattcatc gaagcgctcc tccgccagac gcgccggcag atcggcgaag
      181 aggttaccgc cggcaagcgt catggcgcac atcccgcgag ggtatcggcg tttttctgac
      241 gtggatcaat gcgcgacccg cgtcatcgat gcaaggtgag gctggcagcg atcgcttcga
      301 cacgcgagcg cgctctccgg tgatttgaca atttggcaaa aatcgtcaaa tctgtcgtat
      361 gagttcccga gagcggatcc tggaggcggc gatgaccgtc ttcgcgcgct tcggctatcg
      421 gcgcgcgtcg atggaccaga tcgccgagga agtcgcgttg acccggcagg cggtctacca
      481 tcacttcccg ggcaaggaag cgctgttccg cgccgtggtc gaagacctgc acgagggcgc
      541 ctatgaggcc gaggcggcgg ccgggcggca ggaagagcag gcgggacgca gccttgccga
      601 catcctcgcg gcgcagatcg atgcgcgctt tcgctacatc atcgactgtg tcgagacgtc
      661 gccttatgcg gaagagcttc tctcggagca tcagctccag acccgcgacc tctatgagag
      721 cttcatcgag cacaatatcg gcttgcgcgt cgagacgatc gatcgggtct gcgccgctca
      781 agggctggcc ctgcgccccg acatgacgtc gctcgatctg gcgcgatgca tccagatcgc
      841 cattcgcggc ttcagcgcga cgcaaacgaa tgcggacgtc ctcgccgatc tcgaccgcat
      901 ggtgcgcctg ctcgtcgccg gcgccgtagc ccccggcgcg gccaggacgg cccgagccag
      961 caagaggagt ctccgatgag cagcgttacc gtcaacggcc gccgtcagac cttgcccgac
     1021 gatgccgatg ctctcctggt cgacgtgctg cgccacgatc tcggcctcac cggaaccaag
     1081 ctcgtctgcg cggccggcgt ttgcggcgcc tgcacggtgc tgctcgacgg cgcgccggtg
     1141 gcgagctgcc ttctgccggc gagcgcagcg gcgggcaaaa ccgtcaccac ggtcgaaggc
     1201 atcggcaccg cgaagctcca tgcggtgcag aaagccttca tggcgctcga tgccttgcaa
     1261 tgcggcttct gcacgccggg ctttgtcgtc gaggccgtcg ccttctacga gcattggcgc
     1321 aagacgcgaa gcggcgccac gccgacgcgg gaggagattg gtgctgcgct ttcgggacat
     1381 ctctgccgct gcggcgctta cgacaacatc ctgcgcgcgg tcgcggcagc ctgcgccggc
     1441 cgcttcgagg gcgacgacgt gacttcgccg cgcatcgagg cgcgggagaa agtcacgggc
     1501 gccgcgaaat acaccgtcga catccagcat gaggggcagc tcgagggcgt cgtccttcgc
     1561 tcggcggtcg cgcatgcccg catcgccgcg ctcgatctcg ccccggcgct ggcagtgccg
     1621 ggggtgaagg cggcgatatc gctgctcgac gaggatcgca ccgtccgcta tgtcggcgag
     1681 ccgattgccg ccgtcgctgc cagcgaccgc aagacggcca agcgggccgc ggcggcgatc
     1741 aagatcgtct atgagcctct gccctccgtc atcggcctcg acgccgcgcg caagccggat
     1801 gccgcggtgg tcttcgggaa atcgcgcaag cgcgccggca acgtctcgga aggaacggcg
     1861 atgccgacgc cgtggaaggg caatgtgcgg gggccgtcgg cgcctttctc gcatggcaag
     1921 agcaatgcgc ggaaatggct ggcagcggcg cgcgccgagc acgatccact gctcgtcgaa
     1981 gcgacgttcc gcaccggcgc gcaatcgcat acctgcctcg agccgcatgc ggctgtcgcc
     2041 cgcttcgatg gcgagcggct gaccgtgcat gtctcgacgc aagcggtgtt ccacctcatg
     2101 gagctcatcg ccaagcgctt caagctcgcg ccggacaagg tgaaagtcat tgccgagcat
     2161 gtcggcggcg gcttcggctc caaagccacg ctcggcatgg agaccatcgt cgcgatcgag
     2221 ctggcgcgaa aggcgaaagc cccggtgcgc gtggttttcg atcggcatga ggagctgtcc
     2281 gtcaccggct atcgcccggg cgctgagctc aatatcgcac tgctgccgtc gtcgcgcggc
     2341 catctcaggg ctttgacgct ctcggcctat gccgacaccg gcgctgccgt gaactcgacg
     2401 atcgccgcgc tcgcacgctt gatctatccg gccgaggcga aggagctcgc cgacttcgac
     2461 gtcgtcagca acttgccgcc cggtgccccc ttccgcggcc cgggcggacc accgatggcc
     2521 ttcgcgctcg agcaggcggt cgatgaagcg gcgctccgct tgaagatcga cccgatcgcg
     2581 ctgcgcaggc gctgggacca caatcccaac cgccgtcgcc tttatgattg ggccgccggg
     2641 ctcgatacct ggcggcgcgc gccggcagcg ggcgcgcaga ccgggcgcta ccggcgtggc
     2701 gtcggcgtcg cggcgggata ctggctccat ctttggcaac ccggctcgaa agtcgagctc
     2761 gcggtcaaaa gcggccgcat cgtcgcgagc acggcggtgc aggacatggg cacgggcatt
     2821 cgctcggtga tcgccgacac catcgcccgc gaattcgggc tcgagcccca ggagatcgag
     2881 gtccgcatcg gcgactcctc gcttcccgaa gggccggcgt cgggcgggag ccggaccacg
     2941 gcctccatca tcccgccggc gctgctggcc gtgcggaagc tcaagaccgc gatcgagcac
     3001 agctcgaagg gcgcgccgat gccgggctcg aacgcaccct ggcgtgcggt gatcgccgcc
     3061 acgcccgacg tccaggtcgc ggcggagcgg cccgaggacg accggcgaac ggcggccggc
     3121 atgcactcgc cgctcaaaga ggtgggtttc atcggcacgg tgttcggctt catgatgcgc
     3181 cgcttctcca acatcgtcat cggagcgggc gtgcccagct cggtgcaggt gatcgaggtc
     3241 gaggtggaca cgcttctggg ccgcgtgcgc gtgctgaccg cccacacggg catcgccgtc
     3301 ggcgagatcg ccgcgccggc gctcgcgaag agtcaggccg ccggctcggt catccaaggc
     3361 atcggctacg cgctctatga agggcgcgag gtcgatccgc agagcggcga catcctgacg
     3421 gcgggactcg aggattatcg catccccggc atcgccgaca cgccggtcat cgacgtgcat
     3481 ctcgacgaag gcggattcgg ccatgtgctg ggcggcagcg ccggcctggg cgaagtggcg
     3541 acggtcccga cggcggcggc gctggcgaat gccattcgcc atgccgtcgg cgtgcgtccc
     3601 caagagattc cgctccgccc cgatcggctt ctcggcttgt tgaacgcgcg gaggagcgca
     3661 tgagcgccac cgatgccgaa ttccgtgcgg ccggcaccga tctctcggag cgacgtcgca
     3721 gcggcctctc gcgcggaacg ctgatcgatc tcgccgcgtc ggccgagatg acggggatcg
     3781 aatggggcgc cgacggcgcg gcgcgcatcg gcgctttgac gcgcatcgcc gccatcgccg
     3841 ccgatctgcg catggcgatg gcctatcccg gcctcgtcgc ggcggccggc gggctcgcga
     3901 cgccgcagat ccggcatctg gcgacgctgg gcggcaatct cgcgcaacgc tcgcgctgct
     3961 gggattaccg caacccgcag atcgcctgcc tcaagaaagg cggagcgacc tgcccggcgc
     4021 gcgacggcaa tcatctctac ggcgtggcct tcgatctcgg cccttgcgtc gcgccgcatc
     4081 cctccaccat ggctgcggcg ctcctcgc
//
LOCUS       DASYNS010000466         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4175551, whole genome shotgun sequence.
ACCESSION   DASYNS010000466 DASYNS010000000
VERSION     DASYNS010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4175551"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /gene="hisB"
                     /locus_tag="VGP50_14510"
     CDS             <1..591
                     /gene="hisB"
                     /locus_tag="VGP50_14510"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEV8028643.1"
                     /translation="PARQAGVERITRETKIRGAVNLDGTGACDVATGIGFLDHMLEQF
                     ARHSLIDLTLRAEGDLHIDFHHTTEDSGYVVGEAVSKALGDRRGIARWGDALIPMDET
                     LTRVALDASNRPYLIWKVVFTKPKLGEMDTELFKEWFQAFAQSAGVTLHVETLYGENN
                     HHIVESCYKGLARSLRQAVAIDPRRADQVPSTKGVL"
     gene            601..1242
                     /gene="hisH"
                     /locus_tag="VGP50_14515"
     CDS             601..1242
                     /gene="hisH"
                     /locus_tag="VGP50_14515"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEV8028644.1"
                     /translation="MTTVVIDYGSGNLRSAAKAFERAARDAAIDEEVLVSSDAAQVAA
                     AERVVLPGVGAFADCRRGLAAVPGMEAALDEAVRRRGRPFLGICVGMQLMADRGREFE
                     TVEGLAWLPGEVVAITPDDPTLKIPHMGWNEVEPRRTHPVLDGIERGAHAYFVHSYCL
                     ACAAPGDVLATADYGGDIAAVVGRDNLLGTQFHPEKSQAVGLRLIANFLRWRP"
     gene            1239..1772
                     /locus_tag="VGP50_14520"
     CDS             1239..1772
                     /locus_tag="VGP50_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8028645.1"
                     /translation="MTGTPAVSATVEPLKALEGTDLQDLCDAADAAIKAGGGFGWLRA
                     PPRHIMEAFWRGVLLVPERRLFVARLDRTICGSAQLVRPPRNNEAQAFAAQMISNFIA
                     PWARGHGLARELVVAVEDAARASGFSILNLDIRDTQTAAIQLYESLGYERWGTHPVYA
                     RVDGQPIPGHFYFKRLS"
     gene            1769..>1848
                     /locus_tag="VGP50_14525"
     CDS             1769..>1848
                     /locus_tag="VGP50_14525"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391342.1"
                     /note="catalyzes the formation of
                     5-(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino-l-
                     (5-hosphoribosyl)imidazole-4-carboxamide from
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEV8028646.1"
                     /translation="MILYPAIDLKGGQCVRLLRGEMSAATV"
BASE COUNT          278 a          629 c          617 g          324 t
ORIGIN      
        1 cctgcgcgtc aggcaggggt cgagcgcatc acgcgtgaga ccaagatccg cggcgccgtc
       61 aatctcgacg gcaccggtgc gtgcgacgtc gcgaccggca tcggcttcct cgaccacatg
      121 ctggagcagt tcgcgcgcca cagcctcatc gacctgacgc ttcgcgccga aggggatctg
      181 catatcgatt ttcatcacac gaccgaggac tcgggctatg tcgtcggcga ggcggtctcg
      241 aaagccttgg gcgaccggcg cggcatcgcg cgctggggcg acgcgctcat cccgatggac
      301 gagacgctga cccgcgtcgc cctcgacgcc tccaaccgac cctatctgat ctggaaagtc
      361 gtcttcacca agcccaagct cggcgagatg gacaccgagc tcttcaagga atggttccag
      421 gccttcgccc agtccgcggg cgtgaccttg catgtcgaga ctctctatgg cgaaaacaat
      481 caccatattg tggagtcctg ctacaagggc ttggcgcggt ccttgcggca ggcggtcgcg
      541 atcgatccgc gccgtgccga tcaggtaccc tcgacgaagg gcgttctcta gaggatttcc
      601 atgaccacgg tggtgatcga ttacgggtcg ggcaatctcc gctccgcggc caaggctttc
      661 gagcgcgcgg cgcgcgacgc cgccatcgac gaagaggtgc tcgtgtcgag cgacgccgcg
      721 caggttgccg cggccgagcg cgtagtgttg ccgggagtcg gtgcctttgc cgattgccgc
      781 cgcggccttg ccgccgtgcc cggcatggaa gcggcgctgg acgaggcggt gcgccgtcgc
      841 ggccggccct tcctcggcat ttgcgtcggc atgcagctca tggcggaccg cggtcgcgag
      901 ttcgagacgg tggaggggct cgcctggctg ccgggtgagg tggtggcgat cacgcccgac
      961 gacccgacgc tcaagattcc gcatatgggc tggaacgagg tcgaaccgcg gcggacccat
     1021 cccgtgctcg acgggatcga gcgcggcgcc catgcctatt tcgttcacag ctactgcctc
     1081 gcttgcgccg ccccgggcga tgtgctcgcg acggccgatt atggcggcga cattgccgcc
     1141 gtggtcggcc gcgacaatct cctcggcacg cagtttcatc cggagaagag ccaggccgtc
     1201 ggcctccggc tcattgccaa cttcctccgg tggcgcccgt gaccggaacc cccgcggtca
     1261 gcgcgacggt cgagccgctc aaggctctcg agggcaccga tctccaggac ctctgcgacg
     1321 ccgccgatgc ggcgatcaag gccggcggcg ggtttggctg gctgcgcgcg ccgccccgac
     1381 acatcatgga ggcgttctgg cgcggcgtcc ttctggtgcc ggagcggcgg cttttcgttg
     1441 cccggctcga ccgcacgatc tgcggctcgg cgcagctggt ccgtccgccc cgcaacaacg
     1501 aggcgcaggc cttcgcggcg cagatgatca gcaacttcat cgcgccctgg gcgcggggcc
     1561 acggacttgc ccgcgagctc gtcgtcgccg tcgaggacgc ggcgcgcgcc tcgggcttct
     1621 cgatcctcaa tctcgacatc cgcgatacgc agaccgccgc cattcagctt tacgagtcct
     1681 tgggctatga gcgctggggc acgcatccgg tctatgcccg ggtcgatggc cagccgatcc
     1741 ccggacattt ctatttcaag cgcctctcgt gatcctctat ccggcgatcg atctgaaggg
     1801 cggacaatgt gtacggttgt tgcgcggcga gatgagcgcg gccaccgt
//
LOCUS       DASYNS010000467        18063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2928548, whole genome shotgun sequence.
ACCESSION   DASYNS010000467 DASYNS010000000
VERSION     DASYNS010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 18063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18063
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2928548"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VGP50_14530"
     CDS             <1..1068
                     /locus_tag="VGP50_14530"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV8028647.1"
                     /translation="DCGDFGRNMEDALRLSRYEGFPARRAQSRMRGKLRGIGIANAIE
                     RAAAPGLEFAEIRFTPSGTALVLMGTKNQGQGHETTFRQIVHERLGLAPGDVQYIDGD
                     TDRVAYGMGTMGSRSTVIGGTALWLAAGKVIAKGTKIAAHLLEAAPADIAFAEARFTV
                     VGTDRGVDLKEVARTAFRPGKLPPEIEPGLYETGTFSPGADTFPNGSHVCEVEVDPET
                     GAVTLLTYTVVDDVGTVVNPMGLEGQIHGGLVQGLGQALMEQVVYDPRSGQLLTGSFM
                     DYAMPRADNICDIAVASNPVPTKLNPLGVKGAGEAGTVGALPAVMNAVLDALAPLGID
                     ALDMPATPERIWRAIEEAKVR"
     gene            1091..1966
                     /locus_tag="VGP50_14535"
     CDS             1091..1966
                     /locus_tag="VGP50_14535"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028648.1"
                     /translation="MGTIAGAAIVSHSPSLMMPDAERIAAGAGRDSDLIAGFARLRQK
                     LDAARPDTLVIFDTHWITTNMHVVAGAAYFRGIYTSDEMPHVLHGIAYDYPGAPELAL
                     LVEEVARERGFAALDAVDPHMANHYATLNVVAKLRRDEKILSVGCCQNATLDHYLQMG
                     EIVGEAIARSPARAALLASGALSHVMTVYDAPRKNPRVFHPDNVSDPRHVRLDHEVIA
                     LFAEGRHDRVIERYPELRAAGYEGRGAHYLQMVGALGGRRCRASGIALSEYENARGTG
                     NIHIWFDVGDRAAAA"
     gene            1963..4002
                     /locus_tag="VGP50_14540"
     CDS             1963..4002
                     /locus_tag="VGP50_14540"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEV8028649.1"
                     /translation="MTDNPFFAAWTTPFGLPPFDRLRPEHFPPAFDRGMAEQKAQIAA
                     ITASGEPPSFANTIEAMERSGRLLTRVSRTFFNLSGSNTNDALEAVARDYAPRLAEHH
                     VSIALDPLLYARIADLYARRDTLSLAPDQARLLERTHLGFVRSGALLAPEQKSRMSAL
                     STRLATLHTLFGQNVLHDEKTWQLALDESDLDGLPASIRSAAASTAAERGLAGRYVLT
                     LARSCVEPFLTFSARRDLRQAVYEAWTRRGAHDGPHDNRPLIREILALRAEQARLLGY
                     DNYAAYRLEDTMAREIAAVEKLLLQVWEPAKRKAAVERGELQALARGEGANAPIAPWD
                     WRHYAEKVRRTKYRIDDAEVKPYFVLDNLVRAAFDTARRLFGLAFIERADLPLYHPDV
                     RAYEVRDAADRPVGLFLHDNFARIGKRSGAWMGSYRDQETLDDAVMPIVVNNNNFAKG
                     EPTLLSFDEAETLFHEFGHALHGLLSRVRYPSQSGTAVRRDFVEFPSQIYEHWMSAPA
                     TLRRFAVHHRTGEPIPEELLRRLLAARHFNQGFAAVEYTAAALLDLAFHGAGAADGID
                     VERVERDVLTRIGMPEEIGVRHRPAHFQHLFAGNGYAAGYYAYLWAEALDADGFEAFT
                     ETGDVFDPALAARLRAIYAAGDTRDPMELYVAFRGRAPQIDALLRHRGLAGETVP"
     gene            4081..4764
                     /locus_tag="VGP50_14545"
     CDS             4081..4764
                     /locus_tag="VGP50_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8028650.1"
                     /translation="MTTASPTSTGRWRRRKEARPGEILAAALDCFAERGFAATRLEDV
                     AQRAGVTKGTLYLYFPSKEELFKAVVREAIVAGIARSEARIGDASEPGPQLLGYVLRS
                     WAAQAETPGSAISKLMIAEAGNFPELARFYLDEVIHRGRSLVRRVLTAGIARGEFRPV
                     DVEAAVTCVIAPLILAMLWRHSFAQHDVAFDVEALCRVHLELLCEGLVPVEHRKQTAR
                     PRRRKQGGV"
     gene            4764..5873
                     /locus_tag="VGP50_14550"
     CDS             4764..5873
                     /locus_tag="VGP50_14550"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV8028651.1"
                     /translation="MSLNPSPRAERPTRPADAPLRPAPGTERRPLFRRPLVLLLAAVL
                     LIAIIVGGVIWWLDARRYESTDDAFIDAHMGRVAPQVPGRVLHVLVSDNQPVTPGQPI
                     LEIDPVDLQAKLDQATANETQAGAALAQAKAQLAVSQANTEEAQAQVAVAEANATNAA
                     TQLKRDQALAAQQVVSRQQLDNDTANARSTGANLAAAQKRVVSAQAQEVVAAAQVAAA
                     EANLKAASAQLEQARLNRSYAQIAAAETGRVAHINVAVGDYVQAGQNLMALVPSRIWI
                     TANFKETQLDLMRPGQPVAIHVDAYPGKIFHGHVDSLQPGSGAVFSLLPPENATGNYV
                     KVVQRVPVKIVFDDPADPDRPLGPGMSVEPQVTVR"
     gene            5870..7480
                     /locus_tag="VGP50_14555"
     CDS             5870..7480
                     /locus_tag="VGP50_14555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEV8028652.1"
                     /translation="MSAVIEGAAVAPGARQRRGANPWVVAPTVGFAAFMEVLDISIAN
                     VALQHIAGSLAASTEESTWVLTSYLVTNAIVLPMSGWLASTIGRRRYFLGCIIGFSLT
                     SLLCGLAPSLGLLIVARGLQGITGGGLQPTSQAILADTFPPERRGQAFAAYGLAVVFA
                     PAIGPTLGGWITDNFSWRWVFLLNVPVGAVLSFLAARVLVDSPEEVAQRKARRAQGLS
                     VDYLGFALLVIGMCALQVVLDKGQEDDWFGSVFITDLVITSTLALAAFIVWELRHADP
                     IVDLRLLANRNFGIGSMLMFMLGFILLGSTVLLPLFVQTMLGYTATDAGLVISPGGFA
                     IMAIMPIVGALVARVDARLLIVVGLVTASLALWRMTSFDLDVDYATVAWARVYQSLGL
                     AFLFIPINTVAFLGLPPAKSGNASAIINMMRNVGGSFGIALATTLLARRQQSHQNFLV
                     AHVTPFSSAYDATVQSLQQAFLTQSASAADALIQAQATVYAMVQKQASMLAFIDVFWA
                     MGALFLVMVPLVLLMRKPEAGGGPPPAH"
     gene            complement(7488..7850)
                     /locus_tag="VGP50_14560"
     CDS             complement(7488..7850)
                     /locus_tag="VGP50_14560"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV8028653.1"
                     /translation="MSDVEAFPGAPRRRGPSSGRKRRQGSPPRSPTPLDVHLGARLRL
                     LRIARGFSREEMAGLVGITVRDLRDQERGAKRIRAKHLVEYAEFLEVRLSRFFADVGP
                     RPVPDRKLPSVSSLIADL"
     gene            8153..11098
                     /locus_tag="VGP50_14565"
     CDS             8153..11098
                     /locus_tag="VGP50_14565"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV8028654.1"
                     /translation="MPPLNQETAAPTRPPIGDARPSGDEAETVFAFGEYRVFPRQRRF
                     LGGRAPIELGSRAFDVLMALVEAEGRLVAKSELRKRVWPSTVVDDHNIEVQISALRKA
                     LGGDRDLIRTEAGRGYRLAATITLVTAGSASMPPGSTSLAARPPMTNLPTTISSLVGR
                     GREVPELLELVETHRLVTLTGAGGIGKTQLGLEVARLALPGFADGARVAELGPLVDPE
                     LILDAIARALGIESGFNRRPADQLAAVLGRKRLLLMLDNCEHVVESAARIAETLLRGA
                     PGLHILATSQEPLAAEGERVYRVTPLEVPVTDGASAALALEHSAVELFVERAQAVDPS
                     FVLDDHTASHVSKICRQLDGIPLTIELAAARVATIGVEALAARLDDRFRLLTGGRRTA
                     LPRHHTLRATLDWSYGLLLEAERAVLRRIAIFSGSFTLDGAGAVAAGGPIDGAQAANH
                     VTDLVSKSLVTFDVRGSLPRYRLLETTRAYALDKLAESGEFAAVVRRHARYYHELLEA
                     AATGWHTAPARELVAQYAPEIDNIRAALAWAFEPQGDAEIGIALAAASVPLWALLSML
                     GECRDLAGRALARLARGPGGQTRQEMVLQVALGKSSIWANGPAGTSRIALQRAIDLAE
                     QVGDAECLLQALHVMWGYVLRLGEFGASLDVAERFCRVAEKTADRAAALIGTRLKGVS
                     LYYFGEHAAARAAMERVLDASLAGLYGASMVRFGLDQRIAAFGNLARILWLQGFPEQA
                     ARAAASGVAEARALNHANSLCVALCDGACGLAALAGEVHMVEELATTLIDCAEKHGLE
                     IWRSDGLAFKGWVAVRRGDNELGIHLLRRTFDSFRAKRVELRYTIFVEALVSALAASG
                     RLAEGMAVIEQGLDGSSRHERRWCIPELLRIRGELALGDSRAAEAERDFVEAIDLARQ
                     QDLRSWQLRAATSLAGLQHRQGRAAEARDLLDPIYRSFSEGSCTRDLMAAKALLDAMS
                     SRHSGAADR"
     gene            complement(11074..11820)
                     /locus_tag="VGP50_14570"
     CDS             complement(11074..11820)
                     /locus_tag="VGP50_14570"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV8028655.1"
                     /translation="MSESLFGAGRRDRPPVHRLWIEAMSAVTPTPRRVALAARRSAPR
                     GDGHPVLLIPAFLRGDRYMLPLRRFLSESGYAVHGWRLGVNVGPTDRILDGVERRLDE
                     IRQKHGRKVSLIGHSLGGATARELAKKRPDDVRLLIVLSSPIHLPTASPLEPVYRLLS
                     RWHSASSPTLYASFNEPPAVPVTAIHTRTDGIVAWESCFEAEGPQRENIEVAGPHSTM
                     ARNPAAWRIIADRLAQSEDDWQPYLSAAPE"
     gene            11925..12653
                     /locus_tag="VGP50_14575"
     CDS             11925..12653
                     /locus_tag="VGP50_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEV8028656.1"
                     /translation="MIVLFTDFGLAGPYMGQVRAVLQRLAPAMPVIDLFADAPMRNPK
                     AASYLLAAYAPWFPAGTVFLGVVDPGVGGERPALVVQADGRWYVGPGNGLFEILHRRA
                     DASEVFEIVWRPATLSASFHGRDLFAPVAALIARGEAPPGAKRPADFARHGDWPDDLA
                     EVIYVDHYGNAMTGLRAALLRPDARLVAGHRRIDRARTFGGMPAGTALWYENSTGLAE
                     IAVNMGRADDALGLAIGSAVQIVS"
     gene            complement(12801..13322)
                     /locus_tag="VGP50_14580"
     CDS             complement(12801..13322)
                     /locus_tag="VGP50_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028657.1"
                     /translation="MRRPPYRRLWRELWSFLFHYPPPPEPAAFAQGGGHVVLVVPPAA
                     LNGDFATRPLRQFLTRCGYRVYGWGLGINWGPTPRLLAGLRRRLRELHRIEGRPVSVI
                     GVSLGGLLARDLAYDCAQEIRQVITLASPFRLPTATTIEPSSPGSLLRAESQCRPAGG
                     TAAGRVDGVLHAR"
     gene            13440..13796
                     /locus_tag="VGP50_14585"
     CDS             13440..13796
                     /locus_tag="VGP50_14585"
                     /inference="COORDINATES: protein motif:HMM:NF042631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6559 family protein"
                     /protein_id="HEV8028658.1"
                     /translation="MALLTWFRKGRAARRYARALPPWLARSYGASEHYSVGQIRAAIT
                     ALGLDGPFVATAYAAFMRREEYDPLAAQLRTKLAYEEARDLFLRFVRPHLRSASGEPG
                     DVHSVPDGWQGPGHGT"
     gene            13858..14481
                     /locus_tag="VGP50_14590"
     CDS             13858..14481
                     /locus_tag="VGP50_14590"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV8028659.1"
                     /translation="MPDFALEMSCKGVVCGIDEAGRGPLAGPVVAAAVILDRRRIPHA
                     LRYGLDDSKKLSPGTREEFAAILARCARIGIGAASVHEIDRINILQATLCAMRRAVAA
                     LGCQPEIALVDGNRPPTLPCAVKTVIGGDGLSLSIAAASVIAKVTRDRLMKQLARRYD
                     GYGWETNVGYGTPEHQAALAALGLTPHHRRSFAPVQLSLKFESSELA"
     gene            14561..15643
                     /locus_tag="VGP50_14595"
     CDS             14561..15643
                     /locus_tag="VGP50_14595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV8028660.1"
                     /translation="MGDVVRLRDDEILVGDCVTLMSRLPDASVDLVFADPPYNLQLAG
                     ELLRPNHSRVAGVEEEWDRFADFPDYDRFTRGWLEAVRRLLKPDGALWVIGSYHNIYR
                     VGAILQDLGFWILNDIVWRKTNPMPNFRGRRFTNAHETLLWCARSEDSRYTFNYEAMK
                     ALNDDLQMRSDWTLPICGGPERLKRGGKKAHPTQKPEALLHRVLLASTRPGDLVLDPF
                     FGTGTTGAVAKRLGRRFVGLERDEDYAALARERIAAIETAPLETVTTPGKREEPRIPF
                     GALVERGLLRPGDVLFDASGRFTAKVRADGTLISAAARGSIHQVGALVQGAPACNGWA
                     FWYVEMKGRPVLIDLLRQQVRAEMAR"
     gene            15773..>18063
                     /locus_tag="VGP50_14600"
     CDS             15773..>18063
                     /locus_tag="VGP50_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028661.1"
                     /translation="MKSLRFYAGVFIVAASGLMLQVVQTRILSVVAWYYMAFLAISVA
                     MFGMTAGALWIYLRRQRFSERTLSHDLAYFSSALAISIVLSSAVQMTLASGVISVSAS
                     ALVVWIEIAACEAVPFFFSGVVIGLALTRSPFPVARVYGADLVGAALGCLLALLLLNL
                     VDGPTALIWVAGLVMVAALCFAGAAIGTAQATPWPASRQLLRLKTILAILVLAALANG
                     SGAFGMRPLVVKGKVEGGETTPLYAKWNTFSRIAVFDLGPSITHLWGPSPLFKPEASL
                     IEQRLMNIDGNAATVSYGLHGDVARAAFLKEDVTNIAHFLPGHERAAVIGIGAGRDML
                     SARVFGVPDITGVELNPILAHLLADIPEFADYSGIAALPGMHVNIDEARSWFARNQDH
                     YDIIQMTLIDTWAATSAGAFTLSENGLYTIEAWQNFVDHLNPQGVFTVSRWYAPDNVN
                     EAGRMISLATATLLKLGASDPSQHIFVASSGHVATLVLSRSPFRAEDTALLKRIAEEK
                     QYRVLLSPGGEPASPVLGRIAGSTSMAALDAYTATLDLDLSAPNDERPFFFNTLPLFS
                     PWRVFNLARHNEFAGAVGGNIWATLTLLFLFLLSLLVVVRTIVYPLAPAIGDVGRRLA
                     VGGSAYFCLIGMGFMMAEIGLVQRMSVFLGHPVYALSIVLFSLILSTGVGSLLCDRFP
                     LATRPQFIAWGLATAAYLFAIPFWLPDVLHNCETGQLLLRASVCVGAISPGGLLMGYG
                     FPTGMRLVTAVDRAPAPWFWGING"
BASE COUNT         2753 a         6292 c         5966 g         3052 t
ORIGIN      
        1 gattgcggcg atttcggcag gaacatggaa gacgcgctgc ggctctcgcg ctacgagggt
       61 tttccggcgc gccgcgcgca atcgcgcatg cgtggcaagc tgcgcggcat cggcatcgcc
      121 aatgccatcg agcgcgccgc cgcgcccggc ctcgaattcg ccgagattcg cttcacgccc
      181 agcggcacgg ccctcgtcct catgggcacg aagaatcagg gccagggtca cgagaccacg
      241 ttccggcaga tcgttcatga gaggctgggc ctggcgcccg gcgatgttca gtacatcgat
      301 ggcgacaccg accgggtcgc ttacggcatg ggcaccatgg gctcgcgctc gaccgtcatc
      361 ggcggcaccg ccctctggct cgccgccggg aaggtgatcg ccaagggtac gaagatcgcg
      421 gcccatctgc tcgaagcggc gccggccgac atcgcctttg ccgaggcgcg cttcaccgtc
      481 gtcgggaccg atcgcggcgt cgatctgaag gaggtcgcgc gcaccgcgtt ccgccccgga
      541 aagctgccgc ccgagatcga gcccggcctc tacgagacgg gcaccttctc gcccggcgcc
      601 gatacctttc ccaacggctc gcatgtctgc gaggtcgagg tcgatccgga aaccggggcg
      661 gtgacgctcc tcacctacac cgtcgtcgac gatgtcggca cggtcgtcaa cccgatgggg
      721 ctcgagggcc agatccatgg cggcctggtt cagggcttgg gccaggcgct gatggagcag
      781 gtggtctacg acccgcgatc gggccagctc ctcaccggca gcttcatgga ctatgctatg
      841 ccgcgggcgg acaacatctg cgacatcgcc gtcgcgagca atcccgtccc gacgaagctc
      901 aatccgctgg gagtcaaagg cgccggcgaa gccgggacgg tgggcgcact gccggccgtg
      961 atgaatgcgg tgctcgacgc gctcgcgccg ctgggtatcg acgcgctcga catgccagca
     1021 acgccggagc ggatctggcg agcgatcgag gaagcaaagg tgcgctgaag gcgcgctgcg
     1081 aggaggagcc atggggacga tcgccggcgc ggccatcgtg tcgcacagcc cgagcctgat
     1141 gatgcccgac gccgagcgga tcgccgccgg cgccggccgc gacagcgatc tcatcgccgg
     1201 attcgcgcgg ctgcggcaga agctcgacgc ggcgcggccg gacacgctcg tcatcttcga
     1261 tacgcattgg atcaccacga acatgcatgt cgtcgccggc gccgcgtatt ttcgcggcat
     1321 ctacacgtcg gacgagatgc cgcatgtgct gcacggcatc gcctatgact accccggcgc
     1381 gcccgagctg gcgctgctcg tcgaagaggt tgcgcgtgag cgcggcttcg ctgcgcttga
     1441 cgccgtcgat ccgcacatgg ccaaccacta cgcgacgctc aacgtcgtcg cgaagctccg
     1501 tcgcgacgag aagatcttgt cggtcggctg ctgccagaac gcgacgctcg accactatct
     1561 ccagatgggc gagatcgtcg gcgaggcgat cgcccgctcg cccgcgcggg cagccctcct
     1621 cgcttcgggc gcgctcagcc atgtgatgac cgtctacgac gcgccgcgca agaatccgcg
     1681 cgtcttccat cccgacaacg tctccgatcc acgtcacgtc cggctcgacc acgaggtgat
     1741 cgcgctcttt gccgaaggcc gtcatgatcg ggtgatcgag cgctatcccg agctgcgtgc
     1801 cgccggctac gagggacgcg gcgcgcacta ccttcagatg gtgggcgcgc tcggcggacg
     1861 ccgctgccgc gcctcgggca tagcgctttc cgaatacgag aacgctcgcg gcaccggcaa
     1921 catccatatc tggttcgacg tcggcgatcg ggcggccgcc gcatgaccga caaccccttc
     1981 ttcgcggcct ggaccacgcc tttcggcctt ccgcccttcg accgcctccg cccggagcat
     2041 ttcccgccgg ctttcgaccg cggcatggcc gagcagaagg cgcagatcgc ggcgatcacg
     2101 gcgtccggcg agccaccgag cttcgccaac accatcgagg ccatggagcg cagcggccgc
     2161 ctgctcacgc gggtcagccg caccttcttc aacctcagcg gcagcaacac gaatgacgcg
     2221 ctcgaggccg tcgcgcggga ctacgcgccg cgcctcgccg agcatcacgt ttcgatcgcg
     2281 ctcgatcctc tcttgtacgc ccgcattgcc gatctctacg cgcggcgcga tacgctgagt
     2341 ctcgcccccg atcaggcacg gctcctcgag cgcacgcatc tcggcttcgt acgcagcggc
     2401 gccctgctgg cgcccgagca gaagtcgcgc atgtcggcgc tctccacgcg gctggcgacg
     2461 ctgcacaccc tcttcggcca gaatgtgctc catgacgaga agacctggca gctcgcgctc
     2521 gacgagagcg atctcgacgg cctgcccgcc tccatccgca gcgcggcagc gagcacggcg
     2581 gccgagcgcg gccttgccgg ccgctatgtc ctcaccttgg cccgctcttg cgtcgagccg
     2641 ttcctgacct tctcggcacg ccgcgatctc cgccaggccg tctacgaggc ctggacgcgg
     2701 cgcggcgctc acgacggacc gcacgacaat cgcccgctca tccgtgagat cctggcgttg
     2761 cgcgccgagc aggcacggct cctcggctac gacaactatg cggcctaccg gctcgaggac
     2821 acgatggcgc gggagatcgc cgcggtcgag aagctgctgc tgcaggtctg ggagcccgcc
     2881 aagcgcaagg ccgcggtcga gcgcggcgag cttcaggcgc tggcgcgcgg cgaaggggcc
     2941 aacgcgccca tcgcgccctg ggattggcgc cactacgccg agaaggtgcg gcgcaccaag
     3001 taccgcatcg acgatgccga ggtgaagcct tatttcgttc tcgacaatct ggttcgcgcc
     3061 gccttcgaca cggcgcgacg gctcttcggc ctcgccttca tcgagcgcgc cgacttgccc
     3121 ctctaccatc ccgatgtgcg cgcctacgag gtgcgcgacg ccgcggatcg gccggtgggt
     3181 cttttcctcc acgacaattt cgcgcgcatc ggcaagcggt cgggcgcctg gatgggcagc
     3241 tatcgcgatc aggagacgtt ggatgacgcg gtgatgccga tcgtcgtcaa caacaacaac
     3301 ttcgccaagg gcgagccgac gctgctgagc ttcgacgagg cggagacgct gtttcacgaa
     3361 ttcggccatg ccctccacgg attgctgagc cgcgtgcgct acccttcgca atccggcacg
     3421 gccgtccggc gcgatttcgt cgagttcccg tcgcagatct acgagcattg gatgtcggcg
     3481 ccggcgacgc tccggcgctt tgccgtccac catcggacgg gagagccgat acccgaagag
     3541 ctcctgcgcc ggctcctggc ggcacgccat ttcaaccagg gctttgccgc ggtggagtac
     3601 acggcggcgg cgcttctcga tctcgctttc catggcgccg gcgcggcgga cgggatcgat
     3661 gtcgagcgcg tcgagcgcga cgtcttgacc cggatcggca tgccggagga gatcggcgtg
     3721 cgccatcgtc cggcgcattt tcagcacctt ttcgccggca acggctatgc cgccggctac
     3781 tacgcttatc tctgggccga ggctttggat gccgacgggt tcgaggcctt caccgagacg
     3841 ggcgatgtct tcgatcccgc cctcgccgcc cgccttcggg cgatctacgc cgccggcgat
     3901 acgcgcgacc cgatggagct ctatgtcgcc tttcgcgggc gtgcgccgca gatcgacgct
     3961 ctgctgcgcc atcgcggact cgccggcgaa acggttccct aaaaagaacc ggcgcaggac
     4021 gggcgtcgcc gcgaacgccg ttcttgacgc agctaataac tttccggtaa gttacttcta
     4081 atgacaaccg cctctcccac gtccaccggc cgctggcgac ggcgcaagga ggcgcgcccc
     4141 ggcgagattc tcgcggcggc cctcgattgc ttcgcggaac ggggctttgc cgcaacacgc
     4201 ctcgaggacg tggcgcagcg cgccggcgtt accaaaggca cgctctatct ctatttcccc
     4261 agcaaggagg agctgttcaa ggcggtggtg cgcgaggcga tcgtcgccgg catcgcccgg
     4321 agcgaggccc gcatcggcga tgccagcgaa cctgggccgc agctcctcgg ctatgtgctg
     4381 cgcagctggg cggcgcaggc cgagacgccg ggcagcgcca tctccaagct gatgatcgcg
     4441 gaggccggga acttccccga gttggcccgt ttctatctgg acgaggtaat ccatcgcggc
     4501 cggtcgctgg tccggcgcgt gcttaccgcc ggcattgcgc gtggcgagtt tcgtccggtc
     4561 gacgtcgagg ccgcggtcac ttgcgtgatc gcgccgttga tcctggcgat gctgtggcgt
     4621 cattccttcg cacagcacga cgtcgctttc gacgtcgagg cgctctgccg cgttcatctc
     4681 gagctcctgt gcgagggcct ggtgcccgtg gagcatcgca agcagaccgc ccgtccgcgg
     4741 cgccgcaagc agggaggtgt ctgatgagcc tcaacccatc tccccgcgcg gagcggccga
     4801 cgcgtccggc cgacgcgccg ctccggccgg cgccgggcac cgagcggcgc ccgcttttcc
     4861 ggcggccact ggtgctgctg ctggccgccg tcctgctcat cgcgatcatc gtcggcggcg
     4921 taatctggtg gctcgacgcg cggcgctatg aatcgaccga cgacgccttc atcgacgcgc
     4981 atatggggcg ggtcgcgccg caggtccccg ggcgcgtgct gcacgttctc gtcagcgaca
     5041 accagccggt gaccccggga cagcccatcc tcgagatcga ccccgtcgac ctccaggcga
     5101 agctcgacca ggcgacggcc aacgagacgc aggcaggggc ggccctggcc caggcgaagg
     5161 cgcagttggc ggtgagccag gccaacaccg aggaagcgca agcgcaggtc gccgtcgcag
     5221 aggccaacgc caccaatgcc gcaacgcaac tgaagcgcga tcaggcgctg gcggcacagc
     5281 aggtggtctc gcgccagcag ctcgacaacg acacggccaa cgcacgcagc acaggcgcca
     5341 atctggcagc cgcacagaag cgggtcgtct ccgcgcaggc gcaggaggtg gtggcggccg
     5401 cccaagtcgc ggcggcggag gccaatctga aggctgcctc ggctcagctc gagcaggcgc
     5461 gcctcaaccg ctcttacgcc cagatcgccg ctgcggagac cggtcgcgtc gcgcacatca
     5521 acgtcgccgt cggcgattac gtgcaggccg ggcagaatct gatggcgctg gtgccgtcgc
     5581 ggatctggat caccgcgaat ttcaaggaaa cccagctcga tttgatgcgc ccagggcagc
     5641 ccgtcgcgat ccatgtcgat gcctatcccg gaaagatctt ccacgggcat gtcgacagcc
     5701 tgcagcccgg cagcggcgcc gtcttcagcc tgctgccgcc ggagaacgcg accggcaact
     5761 acgtgaaggt ggtgcagcgc gtgccggtga agatcgtgtt cgacgatccg gccgatccgg
     5821 acagaccctt ggggccgggc atgtccgtcg agccccaggt gacggtgcgg tgagcgcggt
     5881 gatcgaggga gcggccgtcg ctcccggtgc gcgacagcgc cgcggtgcca acccctgggt
     5941 ggtggctccg acggtgggct tcgccgcctt catggaagtg ctcgatatct cgatcgccaa
     6001 tgtcgcgctc cagcacattg ccggcagcct cgcggcaagc accgaggaat cgacctgggt
     6061 gctgacctcg tatctcgtca ccaatgccat cgtcctgccg atgagcgggt ggctcgcctc
     6121 gaccatcggc cgcagacgct acttcctcgg ctgcatcatc ggcttcagcc tgacgtcgct
     6181 cttgtgcggc ctggcgccga gccttggcct cctcatcgtc gcgcggggtc tgcaggggat
     6241 caccggcggc gggctgcagc cgacctcaca ggcgatcctc gccgacacct tcccgcccga
     6301 gcgccgcggc caggccttcg ccgcctatgg gctcgcggtc gtgttcgcgc cggcgatcgg
     6361 accgacgctg ggtggctgga tcaccgacaa cttcagttgg cgctgggtct tcctgctcaa
     6421 cgtgcccgtg ggcgccgtgc tttccttcct ggcggcgcgc gtgctcgtcg attcgcccga
     6481 agaggtggcc cagcgcaagg cccggcgcgc gcaggggctc agcgtcgatt acctgggttt
     6541 cgcgctcctc gtcatcggca tgtgtgcgct ccaggtcgtg ctcgacaaag ggcaggagga
     6601 cgattggttc gggtccgttt tcatcacgga tctcgtcatc acctcgaccc ttgcgctcgc
     6661 ggctttcatc gtctgggagc tccggcatgc cgatccgatc gtcgatctca ggctgctggc
     6721 gaaccgcaat ttcggcatcg gcagcatgct gatgttcatg ctggggttca ttctgctcgg
     6781 cagcaccgtg ctgctcccgc tcttcgtcca gacgatgctc ggctatacgg cgaccgacgc
     6841 cgggctggtg atatcgccgg gcggcttcgc catcatggcg atcatgccga tcgtcggcgc
     6901 gctcgtcgcg cgcgtcgatg cgcggcttct catcgtcgtc ggcctcgtca ccgcttcgct
     6961 cgcgctctgg cggatgacca gcttcgatct cgacgtcgac tacgccaccg tcgcctgggc
     7021 gcgcgtctat caatcgctcg gtctcgcttt cctcttcatt cccatcaaca cggtggcgtt
     7081 tctcgggctg ccgccggcca agagcggcaa cgcgtcggcc atcatcaaca tgatgcggaa
     7141 cgtcggcggc agcttcggca ttgccctcgc gaccacgctg ctcgcgcgcc gccagcagtc
     7201 gcaccagaac ttcctggtgg cgcatgtgac gcccttcagc tcggcctatg acgcgaccgt
     7261 ccagtctctg caacaggcct tcctgacgca atccgccagc gctgccgacg cgttgatcca
     7321 ggcgcaggcg accgtctatg ccatggtgca gaagcaggcg tccatgttgg cgttcatcga
     7381 cgtgttctgg gcgatgggcg cgctcttcct ggtgatggtg ccgctggttc tgctcatgcg
     7441 caaaccagag gccggcggcg ggccgccgcc ggcgcattga cgctgcgcta tagatcggcg
     7501 atgagcgaag agaccgaggg cagcttgcga tcgggaaccg gccgcggccc gacatcggca
     7561 aagaaccggc tcagccggac ttcgagaaac tcggcatact cgacaagatg tttggcgcgg
     7621 atgcgtttcg cgccccgctc ctggtcgcga aggtcgcgga cggtgatgcc gacgaggccg
     7681 gccatttcct cgcggctgaa gccgcgcgcg atccgcaaca agcgcagccg cgcgcccaaa
     7741 tgcacgtcga gcggagttgg cgatcgaggc ggcgagcctt gccggcgctt gcgtccggac
     7801 gacggcccac ggcggcgagg cgctcccggg aaggcctcga cgtccgacat ttgcttctcc
     7861 ctcgtgaaat cgtgcctaga ggttacggca cgaatacccg cgtcagcgcc tcaaaggacc
     7921 tgaagcgcgc tcaaatgatc tcttattcag ggtatttcgc attcggcact atgcgtcgtc
     7981 atggagatgg tctactgcta atagtctatg tagcgctact acgatctatc gctagaatgt
     8041 ttctttttca tctattcgcg tgcatccatc aattgtatag attgtacaaa ttgagtttga
     8101 ccgatgagag acagttgacg gtgagggtcg cgagcgcgcg cccgaaaatg aaatgccccc
     8161 gctgaaccag gagaccgcgg cgccgacccg tccgccgatc ggcgacgctc ggccatcggg
     8221 cgatgaagcg gaaacggtct ttgccttcgg cgaataccgg gttttcccgc ggcagcgccg
     8281 tttcctcggc ggccgggcgc cgatcgagct cggatcgcgt gccttcgacg ttctcatggc
     8341 gctggtcgaa gcggagggca ggctcgtcgc caagagcgag ctgcgcaaac gggtctggcc
     8401 gagcaccgtg gtcgacgacc acaatatcga ggtacagata tcggccctgc gcaaggcgct
     8461 gggcggcgac cgcgacctca tccgcactga agcagggcgc ggctatcggc ttgccgctac
     8521 catcacgctg gtcaccgccg gctccgcttc aatgccgccc ggatcgacgt cactcgcggc
     8581 acgtccgccc atgaccaatc tgccgaccac gatatcgtcg ctggtcggcc gcgggcgcga
     8641 ggtgccggag ctcctggagc tggtggagac gcaccggctg gtgacgctga ccggcgccgg
     8701 cggcatcggc aagacccagc tcggcctcga agtggcgcgc ctcgcgctgc ccggcttcgc
     8761 cgatggggcg cgcgtcgcgg aacttgggcc tttggtggat cccgagctca tcctcgacgc
     8821 cattgcgcgc gcgctcggca tcgaatccgg cttcaaccgg cgtcccgccg atcagctggc
     8881 ggcggtcctc ggccgcaagc ggctgttgct gatgctcgat aactgcgagc atgtggtcga
     8941 gagcgcggcc cgcatcgccg agacgctgct gcgcggcgcg cccgggctgc acattctcgc
     9001 cacgagccag gagccgttgg cggcggaggg cgagcgcgtc taccgcgtga ccccgctcga
     9061 ggtgccggtg accgacggcg cgagcgccgc gctggcgctg gagcacagcg ccgtcgagct
     9121 cttcgtggag cgcgcgcagg ccgtcgaccc gtccttcgtg ctcgacgacc ataccgcgtc
     9181 ccatgtcagc aagatctgcc ggcagctcga cggcatcccg ctcaccatcg agcttgccgc
     9241 ggcgcgcgtc gcgaccatcg gcgtcgaagc gcttgcggcc cggctcgacg accgcttccg
     9301 cctgctcacc ggcggtcgcc gcacggcgct gccgcggcac cacacgctca gagcgacgct
     9361 cgactggagc tacgggctgc tgctcgaggc cgagcgagcg gtgctgcgcc gaatcgccat
     9421 cttttccggg agcttcaccc tcgacggcgc gggtgcggtc gcggcgggcg gcccgatcga
     9481 cggcgcgcaa gcggccaatc atgtgacgga cctcgtcagc aagtcgctgg tgaccttcga
     9541 cgtgcgcggc tctctgccgc gctatcgcct gctcgagacg acgcgcgcct atgcgctcga
     9601 caagctggcc gaaagcggcg agttcgccgc cgtcgtgcgg cggcacgccc gctattacca
     9661 cgagctgctc gaagccgccg cgacgggctg gcatacggcg ccggcgcgcg agctggtggc
     9721 gcagtatgcg cccgagatcg acaacatccg cgctgctctc gcctgggcct tcgagcccca
     9781 gggcgacgcg gagatcggca ttgctcttgc cgccgcctcg gtgccgcttt gggccctgct
     9841 gtcgatgttg ggcgagtgcc gcgatctcgc cggacgagcg ctggcgcggc tcgcgagagg
     9901 gccggggggc cagacgcgac aggaaatggt gttgcaggtc gccctcggga aatcgtcgat
     9961 ctgggcgaac ggaccggcgg gcacgagccg catcgctttg cagcgggcca tcgacctggc
    10021 cgagcaagtc ggcgacgccg aatgcctgct gcaagcgctc catgtgatgt ggggctatgt
    10081 cttgcgcctg ggcgagttcg gcgcttcgct cgacgtcgcg gaaagatttt gccgggtcgc
    10141 cgagaaaacg gcggatcggg cggccgccct catcggcacg cgcctcaaag gagtctcgtt
    10201 gtactatttc ggcgagcacg ccgcggcgcg tgccgcgatg gagcgcgtgc tcgacgcgag
    10261 tctcgcgggc ctctacggcg cgtcgatggt gcgcttcggt ctggaccagc gcatcgcggc
    10321 cttcggcaat ctggcccgca tcctctggct ccagggcttt cccgagcagg ccgcgcgcgc
    10381 ggcagcgtcc ggtgtcgcgg aagcgcgcgc cctcaaccat gccaactcgc tgtgcgtggc
    10441 gctttgcgac ggcgcttgcg gactggccgc cctggccggc gaggtgcaca tggtcgagga
    10501 gctggcgacg acgctcatcg attgcgcgga aaagcacggg ctcgagatct ggcgcagcga
    10561 cggcctcgcc ttcaaaggct gggtcgccgt gcggcggggc gacaacgagc tcggcatcca
    10621 tctcctccgg cgcaccttcg acagcttccg cgccaagcga gtcgagctgc gctatacgat
    10681 cttcgtcgaa gccctggtga gcgcgctggc cgcgagcggc cgcctcgctg aaggcatggc
    10741 ggtgatcgag caggggctcg acggatcctc gcgccacgaa cggcgctggt gcatcccgga
    10801 gctgctgcgc atcaggggcg agctcgcgct cggcgacagc cgggccgcgg aagccgaaag
    10861 ggacttcgtc gaagcgatcg acctcgcccg ccagcaggat ctccggtcct ggcaattgcg
    10921 cgccgcgacg agcctcgcgg ggctgcagca tcgccagggc cgcgccgcag aagcccgcga
    10981 tctgctcgac ccgatctacc gttccttcag cgaaggttcc tgcacacgcg acctcatggc
    11041 cgcaaaggcg ctcctggacg cgatgtcatc gcgtcattcc ggtgcggccg acagataggg
    11101 ctgccagtca tcctcggatt gagcgaggcg gtcggcaatg atgcgccagg cagccggatt
    11161 gcgcgccatc gtgctgtgcg gccccgcgac ctcgatgttc tcgcgctgcg gcccctccgc
    11221 ctcgaagcag ctctcccagg cgacgatgcc gtcggtccgg gtatggatcg cggtcacggg
    11281 taccgccggc ggctcgttga agctcgcata gagggtcggc gacgacgcgc tgtgccagcg
    11341 cgagagcagg cgatagacgg gctcgagcgg actcgccgtc ggcaagtgga tcgggctcga
    11401 gagcacgatc agcagccgga cgtcatcggg ccgtttcttg gccagctcgc gggcggtcgc
    11461 gccgcccagg ctatggccga tcaagctcac tttccggccg tgtttctgcc ggatctcgtc
    11521 gagccgccgc tcgaccccgt cgaggatccg gtcggtcgga ccgacattga cgccgagtcg
    11581 ccagccatgg acggcatagc cgctctccga gaggaaccgc cgcaacggca gcatgtagcg
    11641 gtcgccgcgc aagaaagcgg ggatgaggag gaccggatgg ccgtcgcccc ggggcgcgct
    11701 tcgccgcgcg gcgagcgcca cgcgccgcgg cgtcggcgtt accgccgaca tcgcctcgat
    11761 ccagaggcgg tgcacgggtg gacgatcgcg ccggccggcg ccgaacaggg attcgctcat
    11821 ggcggttgaa tcttggtgct cgacgtccgc gcgtcaaggt agcgcggagc cgcttgccgg
    11881 agacccgctt gtcatgacga gcacctgatc ggcatattcg atcgatgatt gtgctcttca
    11941 ccgattttgg tctcgccgga ccctatatgg gccaggtcag ggcggtgctg cagcgtttgg
    12001 cgcccgcaat gcccgtgatc gatctcttcg ccgatgcccc catgcgcaat cccaaggccg
    12061 cttcctatct gctcgccgcc tatgcgccgt ggtttccggc cggcaccgtc ttccttgggg
    12121 tggtcgatcc gggtgtcggc ggcgagcggc cggctctcgt cgtccaggcc gacggccgct
    12181 ggtatgtcgg tcccggcaac gggctcttcg agatcctgca ccggcgtgcc gacgcgagcg
    12241 aggtgttcga gatcgtctgg cgacccgcga ctctctcggc gagctttcat ggccgcgatc
    12301 tcttcgctcc ggtggccgca ctgatcgccc gcggcgaagc accgcccggc gcgaagcggc
    12361 cggcggattt tgcccgccac ggcgattggc ccgacgatct cgccgaggtg atctatgtcg
    12421 accactacgg caatgccatg accggactcc gggcggcgct gcttcgaccg gacgcgcggc
    12481 tggtcgccgg tcatcgccga atcgaccgcg cccgcacctt cggaggcatg cccgcgggga
    12541 cggcgctctg gtacgagaac tcgaccggcc tcgccgagat cgcggtcaac atgggccgcg
    12601 ccgacgacgc cctcggcctc gccatcggca gcgccgtgca gatcgtgagc tgagcccgcg
    12661 cctatggccg cggcgcaagc acttccgcga gcggaccgag cacctccgga ttccggcaaa
    12721 tcgtgagatg cgcaccagtc acctcgatgg acctgcaggt cgaggagtcg ctcttgcagc
    12781 tctcccaggc gaccacgccg tcatcgcgcg tgaagaacgc cgtcgacgcg accggcagcg
    12841 gtgccgccag ccggacgaca ttgagattcg gctcgtaaaa gcgagccagg agatgacggc
    12901 tcgatggtcg tcgccgtcgg cagacggaac gggctcgcga gcgtgatcac ctggcggatt
    12961 tcctgggcgc aatcataggc taagtcgcgg gcaagcaagc cgcccagact cacgccgatg
    13021 acgctcaccg gtcggccctc gatgcgatga agctcgcgca ggcggcggcg cagacccgcc
    13081 agcaatcgag gtgtcggtcc ccagtttatg ccaaggcccc atccataaac gcggtaaccg
    13141 cagcgcgtca gaaattggcg gagcgggcgc gtggcgaagt ctccgttgag ggctgcgggc
    13201 ggcacgacca gcaccacatg gccgccgcct tgtgcaaacg ccgcgggttc cggcggcggc
    13261 ggataatgaa agagaaacga ccacagctcg cgccagagcc gccggtacgg cggccggcgc
    13321 acgcgtccgc tgtgatcgaa tactccatcg agccgaggca ttggcatcgg cataggcgga
    13381 cggggccacg gttgcaaatc gccgcccagc gagccaccgt cccccacgga gatcaggata
    13441 tggcgcttct gacatggttc agaaaaggcc gcgccgcgcg ccgatatgcg cgcgcgctcc
    13501 cgccgtggct tgcccgcagc tatggcgcgt ccgagcacta ctccgtcgga cagatccgtg
    13561 ccgccatcac ggctctcggc ttggacgggc cattcgtcgc gaccgcctat gccgcgttca
    13621 tgcgccgcga agagtatgac ccgctcgcgg cgcagctgcg gaccaagctc gcttacgaag
    13681 aagcgcgaga cttgttcttg cgctttgtgc ggccgcacct gcgttcagcc tctggcgaac
    13741 cgggcgatgt gcattccgtc ccggacgggt ggcaagggcc ggggcacggc acctagcctc
    13801 gcggccgcgg caaaggaacg ccttgccagc gatgcacctg tcgtgtcttc ttccgccatg
    13861 cccgatttcg cgctggagat gtcctgcaaa ggcgtcgtct gcggcatcga cgaggctggg
    13921 cgcgggccgc tcgcggggcc ggtcgtcgcc gccgcggtca tcctcgatcg gcgtcgcatt
    13981 ccgcatgcgc tgcgctatgg gctcgacgac tcgaagaagc tctcgcccgg cacccgcgag
    14041 gagttcgccg cgatcctcgc gcgctgcgcc cggatcggca tcggcgccgc gagcgtccat
    14101 gagatcgacc ggatcaacat cctgcaggcg acgctctgcg ccatgcgccg cgccgtcgcg
    14161 gcgctcggat gtcagccgga gatcgcgctg gtcgacggca atcgcccgcc gactctcccc
    14221 tgcgccgtca agaccgtgat cggcggcgac ggcttgtcgc tgtcgatcgc cgccgcctcg
    14281 gtgatcgcca aggtgacgcg tgaccgcctc atgaagcaac tcgcccggcg ctatgacggc
    14341 tatggctggg agaccaatgt gggctacggc acgccggagc atcaggcggc gctcgcggcg
    14401 ctgggcctca cgccccatca ccgccgctct ttcgctcccg tgcagctatc gctcaaattc
    14461 gagtcgagcg aactggcgta actgcctgga ctgcccatga aattttattg acgctgcgat
    14521 tcgccgcgcg cacagtgccc cgattcgggg ggatgtgcgc atgggggacg tggtcaggct
    14581 gcgcgacgac gaaatcctag tgggcgattg cgtcacgctc atgtcgcgat tgcccgacgc
    14641 ctcggtcgat ctcgtcttcg ccgacccgcc ctataatctg caattggcgg gcgaattgct
    14701 gcgtcccaac cacagccggg ttgccggggt cgaggaggaa tgggaccgct tcgcggattt
    14761 tcccgactat gaccgcttca cccgcggttg gctcgaagcc gtgcggcggc tcctgaagcc
    14821 cgacggcgcg ctctgggtca taggcagcta ccacaacatc tatcgcgtcg gcgccatcct
    14881 tcaggatctc ggcttctgga tcctcaacga catcgtctgg cgcaagacca acccgatgcc
    14941 gaacttccgc ggccggcgct tcaccaatgc ccatgagacg ctgctctggt gcgcgcgctc
    15001 ggaggactcg cgctacacct tcaactacga ggccatgaag gcgctcaacg acgatctgca
    15061 gatgcgcagc gactggactc tgccgatctg cggcgggccc gagcgcttga agcgcggcgg
    15121 caagaaggcg catccgacac agaagcccga ggcgctgctc catcgcgtgc tgctggcctc
    15181 gacgcggccg ggtgatctcg tgctcgaccc cttcttcggc accggcacca ccggggccgt
    15241 cgccaagcgc ctcggccggc gcttcgtcgg gctcgagcgc gacgaggact atgcggcgct
    15301 ggcgcgcgag cgcatcgccg cgatcgagac ggcgccgctc gagacggtga ccacgccggg
    15361 caagcgcgaa gagccgcgca ttcccttcgg cgcgctggtc gagcgcggcc tgctgcggcc
    15421 gggcgatgtg ctgttcgacg ccagcggccg cttcaccgcc aaggtgcggg cggacggcac
    15481 gctcatttcc gccgcggcgc gcggctcgat ccaccaggtg ggcgctctgg tgcagggcgc
    15541 gccggcgtgc aacggctggg ccttctggta tgtcgagatg aagggacggc cggtgctcat
    15601 cgacctcctc cgccagcagg tcagggcgga gatggcgcgc tagtctccgt cgcggacgag
    15661 gcgtctcgcc gccattcacg ttaactcaac catctccggc aagactggcg gcccgccctt
    15721 cgtgcgacat ttcttcgcgc gcctcggaca ggtcaacagg ggagccaagc ccgtgaaatc
    15781 tctgcgcttc tatgccggcg tcttcatcgt ggccgcgtcc ggcttgatgc tccaggtggt
    15841 ccagacgcgc atcctgtcgg tcgtcgcctg gtattacatg gcgtttctcg ccatcagcgt
    15901 ggcgatgttc ggcatgaccg cgggcgcgct ctggatttac ctgcgccggc agcgcttctc
    15961 agaacgcacc ctctcgcacg acctcgccta tttcagcagc gcgctcgcga tatcgatcgt
    16021 cctcagcagc gcggtgcaaa tgaccctcgc ctcgggcgtc atctccgtca gcgccagcgc
    16081 tctcgtggtc tggatcgaga tcgccgcttg cgaagccgtg cccttcttct tttccggcgt
    16141 cgtcatcggc ctggccttga cgcggagccc cttcccggtc gcccgggtct atggcgcgga
    16201 tctcgtcggg gcggcgctgg gctgccttct cgcgctcctg ttgctgaatc tggtcgacgg
    16261 ccccacggcc ctgatctggg ttgccgggtt ggtgatggtc gccgcgctct gctttgccgg
    16321 cgcggccatc ggcacggcgc aggcgacgcc gtggcccgct tcacggcagc tcctgcgcct
    16381 caagacgatc ctcgccatcc tcgtgctggc ggcgctggcc aacgggtccg gcgctttcgg
    16441 catgcggccg ctggtggtca agggcaaggt cgagggcggc gagacgacac cgctttacgc
    16501 caaatggaac accttctcgc ggatcgccgt gttcgatctc gggccctcga tcacgcatct
    16561 ctggggcccg tcgccgctct tcaagccgga ggcctcgctc atcgagcaac gcctgatgaa
    16621 catcgacggc aacgccgcca ctgtcagcta cgggctgcat ggcgacgtcg cgcgcgccgc
    16681 cttcctcaag gaagacgtca ccaatatcgc ccacttcctg cccggccatg agcgcgccgc
    16741 cgtcatcggc atcggcgcgg ggcgcgacat gctgagcgcc cgcgtcttcg gggtgcccga
    16801 catcaccgga gtcgagctca atccgatcct ggcgcacctc ctcgcggaca tccccgaatt
    16861 cgcggattat tcgggaattg ccgcgctgcc gggcatgcat gtcaacatcg acgaggcgcg
    16921 aagctggttc gcccgcaacc aggatcacta cgacatcatc cagatgacgc tcatcgatac
    16981 ctgggcggcg acgagcgccg gtgccttcac actcagcgag aacggcctct acaccatcga
    17041 ggcctggcag aatttcgtcg atcacctcaa tccgcaaggc gtcttcaccg tcagccgctg
    17101 gtacgcgccc gacaatgtca acgaggccgg gcgcatgatc agccttgcga ccgcgacgct
    17161 gctgaagctc ggagcgagcg acccgagcca gcatatcttc gtcgcttcct cgggccatgt
    17221 ggcgacgctc gtgctgtcgc gctcgccctt ccgcgccgag gacacggcgc tgctgaagcg
    17281 catcgcggag gaaaagcagt accgagtcct gctcagcccg ggcggagagc ccgcctcgcc
    17341 ggtgctcggc cgaatcgccg gaagcaccag catggccgcg ctcgacgcct acaccgcgac
    17401 gctggatctc gatctgagcg cgccgaacga cgagcggcct ttctttttca acaccctccc
    17461 gctcttcagc ccctggcgcg tcttcaacct cgcccgtcac aacgaattcg ccggcgccgt
    17521 cggcgggaac atctgggcca cgctcacgct gctcttcctg tttctcctct cgctgctcgt
    17581 ggtggtcagg accatcgtct atccgctggc accggcgatc ggcgatgtcg ggaggcgcct
    17641 cgcggtcggc gggtcggcct atttctgctt gatcggcatg ggcttcatga tggcggaaat
    17701 cggcctcgtg cagcggatga gcgtctttct cggccatccc gtctacgcgc tcagcatcgt
    17761 gctcttctcg ctcattctca gcacgggcgt gggcagcctg ctctgcgatc ggttcccctt
    17821 ggcgacacgc ccccagttca tcgcctgggg cctcgccacc gccgcctatc tcttcgcgat
    17881 accgttctgg ctcccggacg tcttgcacaa ctgcgagacc gggcaactgc tcctgcgcgc
    17941 ctccgtctgc gtcggcgcga tttccccggg cggacttctg atgggctacg gcttcccgac
    18001 cgggatgcgc cttgtcaccg cggtcgatcg agcgccggcc ccgtggttct ggggcatcaa
    18061 cgg
//
LOCUS       DASYNS010000468         7156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4175943, whole genome shotgun sequence.
ACCESSION   DASYNS010000468 DASYNS010000000
VERSION     DASYNS010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4175943"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..2663)
                     /locus_tag="VGP50_14605"
     CDS             complement(72..2663)
                     /locus_tag="VGP50_14605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV8028662.1"
                     /translation="MQPVDTAVVPREQTRAAADASSIRVQGKFFFAGDEKFFIKGVTY
                     GPFALRADGTQFPDDETVTQDFRMMGEMGANTVRVFTAPPLSLLDRAAEAGLKVLAGI
                     PWSEHVTFLDDPLLEADIIRSVTDTVRGLNRHRALLAYLVGNEIPPDIVRWHGPERVR
                     GFLKRLVAAVKQIHPDGLVSYANFPSTEYLTIDFADFLCFNVYLHQEGAFRGYLSRLH
                     NLAVDRPLVLTEFGIDSMREGEPEQARILSWQIRSAFAMGAAGTFVFAWTDEWFTGGH
                     LIEDWAFGLVDRHRVTKPAFSAVKERYTGPIPPALPRYPRVSVVVCSYNAERTMEPCL
                     ASLEKLNYPDYEVIVVNDGSTDSTLAIAERFNYCRIISQQNKGLSVARNVGAEAANGE
                     IVAYTDSDCVADPDWLTYLVAKMESANLVACGGPNFPPPEDSLVPAAVAVSPGGPTHV
                     LLSDDIAEHIAGCNMAFRRDVLLSMGGFDPVYRAAGDDVDICWRFQDAGYVIGFSPAA
                     VVWHFRRNTVKAYIGQQRGYGKAEALVYSKHPFRFNLFGQAKWLGRIYGDLSAALLLS
                     RKPLIYSGVFGRGLFQTLYEPPSSLAAFLPLSFEWNVIAVVLALAGIVTGGWMWLLTV
                     PLLTTWVMCINGALKAPIDKRFRGVKARALVALLIYVGPILRGWERLKWRLKEIRRSE
                     GAAEPVHEARQKARVVWRERAFYLSYWSERGDEKELILGGLMRFLTPQKYFIVADEGW
                     NDWDLKVARGLWSRALVLVCTENHGGMKRLLRVRCALRLSRFSEVVLRCYAVALAAAL
                     ILDAPAAAAVIAIFGLAQGTLVAYRTFEFGKLMHGIIETVARQVELAPVGPGDIGGLP
                     EGERQAA"
     gene            complement(2838..3839)
                     /gene="galE"
                     /locus_tag="VGP50_14610"
     CDS             complement(2838..3839)
                     /gene="galE"
                     /locus_tag="VGP50_14610"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV8028663.1"
                     /translation="MRRAIMVTGGAGYIGSHACKALARAGYDPIAYDSLVRGHREAVR
                     WGPLVEGDLADQALLVETIRRFQVAAVMHFAAFAYVGESMTQPELYFRNNVVNSFGLL
                     EAMHETGVRKMVFSSTCATYGIPETVPIGEDSPQHPVNAYGETKLMIERALHWHGIAH
                     GLAHVALRYFNAAGADPEGETGELHEPETHLIPLVLRAALGQRAAIDVFGTDYPTPDG
                     TAIRDYIHVSDLADAHVRALAYLEQGGASTALNLATGRGCSVREVIAAAERVTGRRVP
                     QREVGRRAGDPPALVADATRAKDLLGWTPRFSDLDTIIASAWNWQRGRNAEPPSHGA"
     gene            4217..5566
                     /locus_tag="VGP50_14615"
     CDS             4217..5566
                     /locus_tag="VGP50_14615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014200328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEV8028664.1"
                     /translation="MRIAMIGTGYVGLVSGACFSEFGASVTCVDKDAGKIARLQRGEV
                     PIFEPGLDQVIETNTRAGRLSFTGELAPAVAGADAVFIAVGTPSRRGDGHADLTYVFS
                     AAEEIARAITGYTVVVTKSTVPVGTGRRVAEIIRGIRPEAEFDVVSNPEFLREGSAIQ
                     DFMRPDRVVIGTESARAQEVMREIYRPLYLLETPMIFTAIETAELTKYAANSFLATKI
                     SFINEIADLCEKVGADVQDVARGIGLDGRIGRKFLHAGPGYGGSCFPKDCLALVRTAQ
                     EAGAPITIIETVTKVNETRKERMAQRIIRACGGSVQGKTIAVLGLTFKPNTDDMRDSP
                     SLTIIPALQQAGATIRAFDPEGMVEAQKVLENVVLCGGAYDAMQGADALVIVTEWNEF
                     RALDLERVKGLLRSPTVIDLRNIYKPADMADAGFFYFSIGRRSVDPARADQRIRVKR"
     gene            5585..6574
                     /locus_tag="VGP50_14620"
     CDS             5585..6574
                     /locus_tag="VGP50_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEV8028665.1"
                     /translation="MNPTRKRVLVTGGAGFLGSHLCERLIAQGDDVLCVDNYFTGRKD
                     NIAHLLPNPNFEAMRHDVSFPLYVEVDEIYNLACPASPIHYQFDPVQTTKTSVLGAIN
                     MLGLAKRVRARIFQASTSEIYGDPIVHPQREDYRGNVNPIGPRACYDEGKRCAETLFF
                     DYHRQHKLKIRVARIFNTYGPRMHPNDGRVVSNFIVQALKGEPITLYGEGTQTRAFCF
                     VDDLIEGFLRLMAAPDDVTGPINLGNPVETTVAELAHKIIALIGSRSKIERRPLPVDD
                     PIQRCPDISRAHDLLNWEPRVPLDAGLERTIAYFDRLLTDAGEKTAKVLQLRA"
     gene            6812..>7156
                     /locus_tag="VGP50_14625"
     CDS             6812..>7156
                     /locus_tag="VGP50_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013639247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV8028666.1"
                     /translation="MADPVVVPMDGPVLVFGGAYSNLEATEAVLAEARLRGIPPRRIL
                     CTGDIVAYAADAKPTLALLRGAGIHMIMGNCEESLAAGSEDCGCGYAEGSACDRLAVE
                     WYAHASRSLDGED"
BASE COUNT         1271 a         2399 c         2352 g         1134 t
ORIGIN      
        1 gccgtccaag accttcctca tcctgagctt gtcgaaggac gcacggccgt cttgcagcca
       61 ttgacacgct cctacgccgc ctgccgctca ccctcgggta gcccgccgat gtcgccgggg
      121 ccgaccggtg cgagctcgac ctgccgcgcc accgtctcga tgatgccatg catgagcttg
      181 ccgaactcga atgtgcggta agcgacgagg gtgccttgcg caaggccgaa aatcgcaatg
      241 accgcggccg cggccggcgc gtcgaggatg agagccgccg ccaatgcgac ggcataacag
      301 cgcagcacga cctccgagaa acgcgagagc cggagcgcgc aacgcacgcg cagcagccgc
      361 ttcatgccgc cgtgattctc ggtgcagacg aggacaaggg cgcggctcca caggccacgc
      421 gcgaccttga ggtcccagtc gttccagcct tcgtcggcga cgatgaaata cttctgcggc
      481 gtgaggaagc gcatgagacc gcccagaatc agctctttct cgtcgccgcg ctcgctccag
      541 taagagagat agaaggcacg ctcgcgccag acgacgcgcg ccttctgccg cgcctcatgc
      601 acgggctcgg cggcgccctc gctgcgccgg atctccttca atcgccattt gaggcgctcc
      661 cagccgcgca ggatcggccc gacatagatg aggagcgcga ccagcgcacg cgccttcaca
      721 ccgcggaagc gcttgtcgat cggcgccttg agcgcgccgt tgatgcacat cacccaggtc
      781 gtgagcagcg gcacggtcaa gagccacatc cagccgccgg tcacgattcc cgccagcgcc
      841 aggacgacgg cgatcacgtt ccactcgaag ctgagcggca ggaaggcggc gagcgaggag
      901 ggtggctcat agagcgtctg gaacaggccg cggccgaaga cgcccgaata gatgagcggc
      961 tttcgcgaca gcaacagcgc cgccgagaga tcgccataga tgcggcccag ccatttcgcc
     1021 tggccgaaga gattgaagcg gaagggatgc ttgctgtaga cgagcgcctc cgccttgccg
     1081 tagccgcgct gctggccgat ataggccttc accgtgttgc gccggaaatg ccagactacg
     1141 gccgccgggc tgaagccgat gacgtagccg gcgtcctgga aacgccagca gatgtcgacg
     1201 tcgtcgccgg cggcgcggta gaccggatcg aagccgccca tgctcagcag cacgtcgcgg
     1261 cgaaacgcca tgttgcagcc ggcgatgtgc tcggcgatgt cgtcggagag cagcacatgc
     1321 gtcggcccgc ccggcgatac cgcgaccgcg gccggcacca ggctgtcctc gggcggcggg
     1381 aagttgggtc cgccgcaagc gacgagattg gcactctcca tcttggcgac gagataagtg
     1441 agccagtcag gatcggcgac gcagtcgctg tcggtatagg cgacgatctc gccgttggcc
     1501 gcctcggcgc cgacattgcg cgccacgctc aggcccttgt tctgctggct gatgatgcgg
     1561 caatagttga agcgctctgc gatcgcgagc gtgctgtcgg tcgagccgtc gttgacgacg
     1621 atcacttcgt agtcgggata attgagcttc tcgagcgagg cgagacaggg ctccatggtg
     1681 cgctcggcat tgtaggagca caccaccacc gagacgcgcg gatagcgcgg caacgccggc
     1741 ggaatcgggc ccgtgtagcg ttccttgacg gcagagaaag cgggcttcgt gacgcgatgg
     1801 cggtcgacga ggccgaaggc ccagtcctcg atgagatggc cgccggtgaa ccactcgtcg
     1861 gtccaggcaa agacgaaggt gccggccgcg cccatggcga aagcgctgcg aatctgccag
     1921 gaaagaatgc gcgcctgctc cggctcgcct tcgcgcatgg agtcgatgcc gaactccgtc
     1981 agcaccagcg ggcggtcgac cgcgagattg tggaggcgcg agagatagcc gcggaacgcc
     2041 ccctcctgat gcagatagac gttgaagcag aggaaatcgg cgaagtcgat ggtcagatac
     2101 tcggtcgagg gaaagttcgc gtagctgacg agaccgtcgg gatggatctg cttgaccgcc
     2161 gccaccagcc gcttgagaaa gccgcgcacg cgctcgggac catgccagcg cacgatgtcg
     2221 ggcgggatct cgttgccgac gagataggca agaagagcgc ggtgccggtt gaggccgcgc
     2281 accgtgtcgg tgacgctgcg gatgatgtcg gcctcgagaa gcggatcatc gaggaaggtg
     2341 acgtgctccg accagggaat gccggccagc accttgagac ccgcctcggc ggctcggtcg
     2401 aggagggaga gcggcggcgc agtaaaaacg cgcaccgtgt tggcgcccat ctcccccatc
     2461 atccggaagt cctgcgtcac cgtctcgtcg tccgggaact gcgttccatc ggcgcgcagc
     2521 gcaaagggtc cgtaggtcac gcctttgatg aagaactttt cgtcgcccgc aaaaaagaac
     2581 ttgccctgca cccggatcga cgacgcgtcc gcggccgcgc gggtttgctc gcgtggcacg
     2641 acggcggtgt cgaccggctg catgatgcga tgaactcccg tgacgtctgg accggcgcgg
     2701 tgcgtccgac agccgccgcg gcccgtgccg cgcccaagct gcgagggcct tgccttgggc
     2761 ggcgatagcg ccgcagaaac accgccctgt caagcgctgc gcgtctcggg catcggcgcg
     2821 gcgacggcca acctgcctca agccccgtgc gagggcggct ccgcattgcg gccgcgttgc
     2881 caattccagg cgctggcgat gatcgtatcg agatcggaga agcgcggcgt ccaacccagc
     2941 aggtccttgg cgcgcgtcgc gtcggcgacg agcgccggtg gatcgccggc gcggcgtccg
     3001 acctcgcgct gcggcacgcg ccgcccggtc acgcgctcgg ccgcggcaat gacctcgcgc
     3061 acggaacagc cgcgtcccgt ggcgagattg agggccgtgc tggcgccgcc ctgctcgaga
     3121 tacgccaagg cgcgcacatg cgcatcggcg agatcactta catgaatgta gtcgcggatg
     3181 gcggtgccgt cgggtgtcgg atagtcggtg ccgaagacgt cgatcgcggc gcgctgcccg
     3241 agcgcggcgc ggagcacgag cgggatcaaa tgcgtctcgg gctcgtgcag ctctccggtc
     3301 tcgccctcgg gatcggcgcc cgcggcattg aaatagcgca gcgccacatg cgcgaggcca
     3361 tgggcgatgc catgccaatg gagcgcgcgc tcgatcatca gcttggtttc gccgtaagca
     3421 ttgaccggat gctgcggcga atcctcgccg atcggcacgg tctcgggaat gccgtaggtg
     3481 gcgcaggtcg aggagaagac catcttccgc acgcccgtct cgtgcatggc ctcgagcagg
     3541 ccgaagctgt tcaccacgtt gttgcggaaa tagagctcgg gctgggtcat ggattcgccg
     3601 acataggcga aggcggcgaa atgcatgacg gccgccacct ggaagcgccg gatcgtctcg
     3661 accaggaggg cctggtcggc gaggtcgccc tccaccaggg gtccccagcg cacggcctcg
     3721 cggtgcccgc gcaccaggct gtcataggcg atggggtcat agccggcacg ggccagcgcc
     3781 ttgcaggcgt ggctgccgat ataaccggcg ccgccggtaa ccatgattgc cctgcgcatc
     3841 gctgctcgcc ctggttgccg tgccataacg ggcgacgctg cgccctgctt ccatcccccg
     3901 cgctgtcata cgacgaaact gccgcctgcg acaagcaatg cccgccgccc ggcgcggcga
     3961 agcgccgcat cgggcaccgc gataccgggc tcgtcgggcc gccctttgct tgacgcggcg
     4021 aaagggatcg tgaataagtg accggcggtt gcccaaaggg taagaagcag cctattgggg
     4081 cgcgtgttaa ccagccttgg agaaacagag cgcagcctga caacggcggc gacatcgcgg
     4141 ccgcagccgg ggaacgttaa ggggaatatt tcacggcgac gtccgttctt ggagcgttgc
     4201 tcggcgagga gtggccatgc gcatcgcaat gatcggcacc gggtatgtcg gactcgtctc
     4261 gggcgcgtgc ttctccgaat tcggtgcgtc ggtgacatgc gtcgacaagg atgccggcaa
     4321 gatcgcgcgc ctgcagcggg gcgaggtccc gatcttcgag ccgggcctcg atcaggtcat
     4381 cgagaccaat acgcgggccg gccgcctctc cttcaccggc gagctcgctc cggccgttgc
     4441 cggcgccgat gcggtgttca tcgccgtcgg cacaccgtcg cgccgcggcg acggccatgc
     4501 cgacctcact tatgtgttca gcgccgccga ggagatcgcc cgtgcgatca ccggctatac
     4561 cgtggtcgtc accaagtcga cggtgccggt cggcaccggc cgccgcgtcg ccgagatcat
     4621 tcgcgggatc cgccccgagg cggagttcga cgtcgtgtcg aacccggaat tcctgcgcga
     4681 aggctcggcc atccaggact tcatgcgtcc cgaccgcgtc gtcatcggca ccgaaagcgc
     4741 gcgcgcccag gaagtcatgc gcgagatcta ccggccgctc tatctcctcg agacgccgat
     4801 gatcttcacc gcgatcgaga ctgccgagct caccaaatac gccgccaaca gcttcctcgc
     4861 gaccaagatc tccttcatca acgagattgc ggatctctgc gagaaggtcg gcgccgacgt
     4921 ccaggacgtc gcccgcggca tcggcctcga cggccggatc ggccgcaagt tcctccatgc
     4981 cggcccgggc tatggcggct cgtgcttccc caaggattgc ctcgccctcg tgcgcacggc
     5041 gcaggaggcg ggcgctccca tcaccatcat cgagaccgtc accaaggtta acgagacgcg
     5101 caaggagcgc atggcgcagc ggatcatccg cgcctgcggc ggcagcgtcc aaggcaagac
     5161 catcgccgtg ctgggcctca ccttcaagcc caataccgac gatatgcgcg acagcccgag
     5221 cctcacgatc atcccggcgc tgcagcaggc gggcgccacc atccgcgcct tcgatcccga
     5281 aggcatggtg gaggcgcaga aggtcctcga gaacgtcgtg ctctgcggcg gcgcctatga
     5341 cgccatgcag ggcgccgacg cgctcgtcat cgtcaccgag tggaacgagt tccgcgcgct
     5401 cgacctcgag cgcgtcaaag gcctgctgcg ttcgccgacg gtgatcgacc tgcgcaacat
     5461 ctataagccg gcggacatgg cggacgccgg gttcttctat ttcagcatcg gccgtcgctc
     5521 ggtggatccg gcgcgcgccg atcagcgtat cagggtaaaa cgataaagca aaaggaagag
     5581 gccgatgaac ccgacaagaa agcgcgtgct cgtcaccggt ggcgcgggat tcctaggctc
     5641 gcatctctgc gagcgtctga tcgcccaggg cgacgatgtg ctgtgcgtcg acaactattt
     5701 caccgggcgc aaagacaaca tcgcgcatct gctgcccaat ccgaatttcg aggcgatgcg
     5761 ccacgacgtc tccttcccgc tctatgtcga ggtggacgag atctacaatc tcgcctgtcc
     5821 ggcctcgccc atccactacc agttcgatcc ggtacagacg accaagacca gcgtgcttgg
     5881 cgccatcaac atgctgggtc tggcgaagcg cgtgcgcgct cggatcttcc aggcctcgac
     5941 ctcggaaatc tatggtgacc ccatcgtcca tccgcagcgc gaggactatc gcggcaacgt
     6001 caacccgatc ggaccgcgcg cctgctatga cgagggcaag cgctgcgccg agaccctgtt
     6061 cttcgactac caccgccagc acaagctcaa gatccgcgtc gcccgcatct tcaataccta
     6121 tggaccgcgc atgcatccca atgacgggcg cgtcgtctcg aacttcatcg tccaggcctt
     6181 gaagggcgag ccgatcacgc tctatggcga aggcacacag acccgcgcct tctgcttcgt
     6241 cgacgatctc atcgagggtt tcctgcggtt gatggcggcg cccgacgacg tcaccggccc
     6301 gatcaatctc ggcaatccgg tcgagaccac ggtggccgag ctcgcgcaca agatcatcgc
     6361 gctcatcggc tcgcgctcga agatcgagcg ccggccgctc cccgtcgatg atcccatcca
     6421 gcgctgcccg gacatcagcc gcgcccacga tctcctcaac tgggagccgc gcgtgcctct
     6481 cgacgccggc ctcgagcgca ccatcgccta tttcgaccgc ctcctcaccg acgccggcga
     6541 gaagaccgcc aaagttctac agctcagggc ttagctctcg cactcaccgg gcagaagcgt
     6601 ggcatgatcc ctctcccctc cgcgggagag ggaggagccc gtcgcgtagc gacgggaggg
     6661 tgaggggcgc tacagcggga tttctccgcc ggcttcgtcg cggaaccacc cctcaccctt
     6721 gcttcgcaat gggcccctcc ctctcccgca aggggagagg gaattagtgc gcatgtgcgg
     6781 tgggagccga gagccgttat agtccgcttt catggcagat cctgtcgtag tgcccatgga
     6841 tgggccggtg ctcgtcttcg gcggcgctta cagcaatctc gaggcgaccg aggcggtgct
     6901 cgcggaagcg cgcctgcgcg gcatcccgcc gcggcgcatc ctctgcaccg gcgacatcgt
     6961 cgcttatgcc gccgacgcga agccgacgct ggcgctcctg cgcggcgccg gcatccacat
     7021 gatcatgggc aattgcgagg agtcgctcgc ggcggggtcg gaggattgcg gttgcggcta
     7081 tgcggaaggc agcgcctgcg accgtctggc ggtcgaatgg tatgcccacg cgtcccgcag
     7141 cctcgacggc gaggac
//
LOCUS       DASYNS010000469         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4490415, whole genome shotgun sequence.
ACCESSION   DASYNS010000469 DASYNS010000000
VERSION     DASYNS010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4490415"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..671)
                     /locus_tag="VGP50_14630"
     CDS             complement(9..671)
                     /locus_tag="VGP50_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028667.1"
                     /translation="MYEQEKWDLRAAGAARSGLDLESVDLVVLPAYSGKTVARRQARV
                     RLLRDYLREQLDDVAGLPALEETASPEGDIAASAPRLRSESVCAKCRGQCCRKGGDHG
                     YISPSTLQRVLAADPALGGGALIDAYVSHVPTRSYAGSCIYHSASGCALPRALRSETC
                     TSFLCDGLKAVSESASQHPERAVVGVFELKDRFPPVTRHVMVVHDGRMDDLRAQRRNA
                     SE"
     gene            complement(747..1508)
                     /locus_tag="VGP50_14635"
     CDS             complement(747..1508)
                     /locus_tag="VGP50_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEV8028668.1"
                     /translation="MIRVGIGGWVFPPWRGGNFYPPGLPQARELAHASGRLTSIEINA
                     TFYGAQKPTSFRRWHSETPDDFVFSVKAPRFATHRKDLGGAKPSIDRFFASGVLELGD
                     KLGPILWQFPQFARFDEQGFADFLALLPPRIEGRAIRHVIEAKHKSFFGEPALALLRR
                     QGVALALIDSDDHPEFPGPTADFVYARLRRSVASEPQGYPPANLDVWAERFRAWRAEG
                     TGDGAPPRDCFVYFINGAKERAPAAAEALIQRLAS"
     gene            complement(1518..2078)
                     /locus_tag="VGP50_14640"
     CDS             complement(1518..2078)
                     /locus_tag="VGP50_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV8028669.1"
                     /translation="MTEQRFKPLSPEEMTPEQRRMAESILAGPRKSLSGPFSALLYSP
                     ETGDQAQKLGAQVRFHNVLPDKLKELAILMVARHWTAQFEWYAHHRLALAAGLDPQIA
                     RDIAAGRRPAALQPDEAAIHDFCAELLETKEVSDATFKAVADRFAEKGVIDLICTLGY
                     YSLVSMTLNVNRFPIPKDAVPLAKLG"
     gene            complement(2181..2414)
                     /locus_tag="VGP50_14645"
     CDS             complement(2181..2414)
                     /locus_tag="VGP50_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEV8028670.1"
                     /translation="MGGLSLWHWLVIIAVVAVLFGGKGKISNLMGDFAKGIKAFKSGM
                     KEEETAGTTPPQVPPPATTAAGAAADRPVHDRG"
     gene            complement(2688..>3947)
                     /locus_tag="VGP50_14650"
     CDS             complement(2688..>3947)
                     /locus_tag="VGP50_14650"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HEV8028671.1"
                     /translation="FCDGSHVAALFSGEKNPKRLPDRRDTYHGQQIVIYDNRGLCAHS
                     GFCTDRLASMFHAASEPFVTPSGGRLDEAIAAVRNCPSGALSFALGGREDRALVDRAR
                     PPAIEVSQDGPYRITGGVRLLSGEVADAPRLQGASLEHYSLCRCGASQNKPFCSGMHW
                     YSGFADPIPNPAHEPTLFEWAGGYPALLRLSHIFYEKYVPQDPLVGPLFARMSPDHPE
                     RVAAWLSEVFGGPKWYTERYGGYERMISEHLNKGITLEQRAHWASLMAQSAEDAGLPA
                     DAEFRAAFVAYIEWGSRIAVENSKPGVQPPPRMPVPRWWWVCGAMPGARPKAKAPGEA
                     DTQPVIALPDAATPVSFAAHIKPLFRAMDRNSMKFAFDLWAYDDVAAHGPAILERIRS
                     GTMPCDGAWPSEKIEVLSRWLAEGRAP"
BASE COUNT          632 a         1274 c         1392 g          649 t
ORIGIN      
        1 tcgccgcctc attcggatgc gttccggcgc tgcgcgcgca ggtcgtccat tctgccgtca
       61 tgcaccacca tcacgtgcct cgttaccggc gggaacctgt ccttgagctc gaaaacgccg
      121 acgaccgcgc gctcgggatg ctgcgacgcg ctctcgctga cggctttgag gccgtcgcat
      181 aaaaagctcg tgcaggtctc gctgcgcagc gctcgcggca gcgcgcagcc ggacgcgctg
      241 tgatagatgc acgacccggc gtagcttcgt gtcggcacgt ggctcacata agcgtcgatc
      301 agggcgccgc cgccgagggc cggatcggcc gcgagcacgc gctgcaatgt gctgggtgag
      361 atgtagccgt gatcaccgcc cttgcggcag cactggccgc ggcacttcgc gcaaacgctt
      421 tccgagcgaa gccgtggcgc tgaggcggcg atgtcgcctt ccggagatgc cgtctcctcg
      481 agggccggca gcccggcgac gtcgtcgagt tgctcccgaa gataatcgcg caggagacgc
      541 acccgggctt ggcggcgggc gacggtcttt cccgaatagg ccgggagcac gacgaggtcg
      601 accgactcga gatcgagacc cgagcgggcg gcaccggcgg cgcgcaagtc ccatttttcc
      661 tgctcgtaca ttctttgcac tccatgcccg ccgcggtcga atttgagtca tcgtacactc
      721 tgttgccgct cgcgcacttg tcaccgtcac gacgccaatc gctggatcag cgcttcggcg
      781 gccgcgggcg cgcgctcctt ggcgccgttg atgaaataga cgaagcaatc gcgtggcggc
      841 gcgccgtcgc ccgttccctc cgctcgccac gcgcggaaac gctcggccca gacgtcgaga
      901 ttggcgggtg gatagccctg gggctcgctc gccacgctgc ggcggagccg cgcatagacg
      961 aaatcggcgg tggggccggg gaactcgggg tggtcgtcgg aatcgatcaa cgccaatgcc
     1021 acgccctggc gccgcagcag cgcgagtgcc ggctcgccga agaagctctt atgcttggcc
     1081 tcgatgacgt ggcggatcgc ccggccctcg atgcgcggcg gcaggagcgc gagaaaatcg
     1141 gcgaagccct gctcgtcgaa gcgcgcgaat tgcgggaatt gccagaggat cgggccgagc
     1201 ttgtcgccga gctcgaggac gccgctggcg aagaagcgat cgatcgacgg cttcgcgccg
     1261 ccgaggtcct tgcgatgggt cgcaaagcgc ggggctttga ccgagaagac gaaatcgtcg
     1321 ggcgtctcgc tgtgccagcg gcgaaagctc gtcggctttt gcgcgccgta gaaagtggcg
     1381 ttgatctcga tcgaggtgag gcggccgctg gcatgggcga gctcgcgcgc ttgcggcagg
     1441 ccgggcggat agaaattgcc gccgcgccag ggagggaaga cccaaccgcc gatgccgaca
     1501 cggatcatcg gcgcaagcta tccgagcttc gcgagcggaa cggcgtcctt ggggatcggg
     1561 aagcggttca cgttgagcgt catcgagacc aggctgtagt aacctagcgt gcagatgagg
     1621 tcgatcacgc ccttttccgc gaagcggtcg gcgaccgctt tgaaggtggc gtcgctcacc
     1681 tctttcgtct ccaggagctc ggcacagaaa tcgtggatag cggcctcgtc gggctggagc
     1741 gctgccgggc gcctgccggc ggcgatgtcg cgggcgatct gcgggtcgag accggcggcg
     1801 agcgccaggc ggtgatgcgc ataccactcg aactgcgccg tccagtggcg cgccaccatc
     1861 aggatcgcca gctccttcag cttgtccgga agcacgttgt gaaagcgcac ctgtgcgccg
     1921 agcttctgcg cctggtcgcc ggtctcgggg ctgtagagca gggcgctgaa cggtccgctc
     1981 agcgacttgc gcgggccggc gaggatgctc tcggccatgc gccgctgctc cggcgtcatc
     2041 tcttccgggg aaagtggctt gaaacgctgc tcggtcatga cgggctcgcg gtctcctctt
     2101 tcgggagccg cacgatatca acgccgcttc gcccgcggaa gagcgcctag gaacgatgga
     2161 tggtcgtcgc gcgccgcggc tcagccgcgg tcatgcacgg gccggtcggc cgcggctccg
     2221 gccgccgtgg tggccggcgg cggtacttgc ggcggcgtcg tgccggcggt ctcctcctct
     2281 ttcatcccgg atttgaacgc cttgatgcct ttggcgaaat cgcccatcag gttggagatc
     2341 ttgcctttgc cgccgaagag aacggcgacg acggcgatga tgaccagcca atgccagaga
     2401 cttaatccac ccatgggaac ctcggattct cagggtcctc tcggaccgaa cagcattctg
     2461 cgcagatggc gccgggtatg gccgtgattg tcaaggggaa acggtctctt ggcaaccagg
     2521 cagcggagcg gccgttatcc tcgaacgcgg caagttcgtc tcccgatgga gacgcctgcc
     2581 caggaggtgg cgtcgcggcg ggtccgtgtc aacaccatcg cgccgggcgc catccgtgcg
     2641 cgggcgctta gaaccaacga tcggcgtctc ccctgcggga gcgcgcgcta tggagcccgg
     2701 ccctccgcca gccagcgact gaggacctcg atcttttcgc tgggccaggc gccgtcgcac
     2761 ggcatggtcc ccgacctgat gcgctcgagg atcgcagggc catgggcggc gacgtcgtca
     2821 taggcccaaa gatcgaaggc gaatttcatc gaattgcggt ccatcgcacg gaacagcggc
     2881 ttgatgtggg ccgcgaaact gacgggcgtg gccgcgtcgg gcaaggcgat caccggctgc
     2941 gtatcggcct ctccgggggc cttggctttc ggccgggcgc cgggcatggc gccgcagacc
     3001 caccaccacc tgggaaccgg catgcgcggc ggcggctgga ccccgggctt ggaattttcg
     3061 acggcgatgc gcgagcccca ttcgatatag gccacgaacg cggcgcggaa ctcggcgtcg
     3121 gccgggaggc cggcgtcctc ggcgctctgc gccatcaacg acgcccaatg ggcgcgctgc
     3181 tcgagagtga tgcccttgtt gaggtgctcg gagatcatcc gctcatagcc gccgtatcgc
     3241 tcggtgtacc acttgggacc gccgaacacc tcgctgagcc acgcggccac gcgctcggga
     3301 tggtccggcg acatccgcgc gaacagaggt ccgaccagcg ggtcctgcgg cacatatttc
     3361 tcatagaaga tatgcgagag gcggagcagc gccggatagc cgcccgccca ctcgaacagc
     3421 gtcggttcgt gggcgggatt ggggatcggg tcggcgaagc cggagtacca gtgcatgccg
     3481 ctgcagaacg gtttgttctg ggaggcgccg caacggcaga ggctgtagtg ctcgagagac
     3541 gctccttgaa ggcgcggggc gtcggctacc tcgccggaca gcagccgcac gccgccggtt
     3601 atccggtagg ggccgtcctg cgagacttcg atggccggcg ggcgcgcccg atcgacgagc
     3661 gcgcggtcct cccgtccgcc gagagcgaag ctgagcgcgc cggaggggca gttgcgcacc
     3721 gcggcgatcg cctcgtccag cctcccgccg ctgggcgtca cgaacggttc ggaggcagcg
     3781 tggaacatcg aggccaggcg gtccgtgcag aagccggaat gcgcgcaaag gccgcgattg
     3841 tcgtagatga cgatctgctg gccatggtag gtgtcgcgcc ggtcaggaag ccgcttgggg
     3901 ttcttctcgc cgctgaagag cgcggcgacg tgggatccgt cgcagaa
//
LOCUS       DASYNS010000470         4915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4019621, whole genome shotgun sequence.
ACCESSION   DASYNS010000470 DASYNS010000000
VERSION     DASYNS010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4019621"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1714
                     /locus_tag="VGP50_14655"
     CDS             <1..1714
                     /locus_tag="VGP50_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8028672.1"
                     /translation="MDASNMLKPALARGELHCVGATTLDEYRKHIEKDAALARRFQPV
                     FVNEPTVEDTISILRGIKEKYEVHHGVRITDGAIVAAATLSNRYITDRFLPDKAIDLM
                     DEAAARLRMAAESKPEAIDELDRRIMQLKIEREALKKESDAASRERLERLEDELEDLE
                     KKSAELTAQWRAEKDKLASAQKLKEQLDQARIELEQVQRRGDWARAGELTYGVIPELE
                     RKLKEAEAVESHRMIDEAVTAEHIAGVVSRWTGIPVDKMLEGEREKLLQMEQNLKRRV
                     IGQDEAVVAVSNAIRRARAGLQDANRPIGSFLFLGPTGVGKTELARALAEFIFADESA
                     MIRIDMSEYMEKHSVARLIGAPPGYVGYEEGGSLTEAVRRRPYQVILFDEIEKAHPDV
                     FNVLLQVLDDGRLTDGQGRTVDFRNTLIVLTSNLGGEILATLPENVDVSEVRNQVMEA
                     VRGAFRPEFLNRLDEILIFRRLSRADMRGIVDIQLRRLDKLLADRKITLAVDEKARGW
                     LAEAGYDPVYGARPLKRVIQRSLQNPLAELILGGRISDGETVDVTVGDGGLAIAGRVA
                     KAAE"
     gene            1804..2250
                     /gene="cynS"
                     /locus_tag="VGP50_14660"
     CDS             1804..2250
                     /gene="cynS"
                     /locus_tag="VGP50_14660"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEV8028673.1"
                     /translation="MTRAELTEKIAVIKRRKGLSWRDIAAKIGPGSPVYYTAALHGQM
                     KLGKAEAEAAGKLLGLDEDEVALLQEVPYRGSLPTAVPTDPLIYRFYELIQVYGTTWK
                     ALIQEDFGDGIMSAIDFDMKIERQPDQKGDRVRVSMSGKFLPYKQY"
     gene            2343..3101
                     /locus_tag="VGP50_14665"
     CDS             2343..3101
                     /locus_tag="VGP50_14665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8028674.1"
                     /translation="MLDGQVAWITGAGSGIGRAGALELAAAGARVALSGRRAEQLEQV
                     AAEIAASGGKAEMFALDVADRSAVAAAAAEILDRCGPVDILVNSAGINVPNRFWKTLT
                     PESFDQVVAVNLNGALYCTHAVLPAMRARRSGLVINISSWAGRFESYLTGPAYNASKH
                     AMVAMTLSLTDEECVNGIRGCVICPGEVATPILRHRPKPPSPEEIARMLRPEDLGRTI
                     RFVAEMPAHVCVNEIVISPTWNRFFMGGKDVARG"
     gene            complement(3117..4409)
                     /locus_tag="VGP50_14670"
     CDS             complement(3117..4409)
                     /locus_tag="VGP50_14670"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEV8028675.1"
                     /translation="MTFLRPPVAVAALFLLVNFPMAPAADLSAARPAAPAPPPAAAAA
                     PAMTAQPPAAAAADPAISHLVQIASGDTLMGLLIDQGVAAADAQDVIDALKPVWNPRE
                     LKTGQKVALQFQAARLQELRLAPAIDRDVIVTRAGDGHFASHAAAHPLVRAPALARGV
                     IRSSLFDAASEAGVPMAILAEMIHAFSYDVDFQREVQPDDSFEIVFDRLYDESGKAVG
                     ADNIIYASMTLSGNTLRIYRYTPAGDTRADFFNAKGESVRKALLRTPVDGARLSSGFG
                     MRFHPILGYTKMHRGVDFASPPGTPVMAAGDGVVETAGYKGDYGNFVLLRHSNSFETA
                     YAHMSRLATHAGQRIHQGQVIGYVGATGRATGPHLHYEVRIHGDAVNPLSIKMQPGPL
                     LAGKDLAAFRTVAASIDHRLPVLRGEPVVAANVTGPPR"
BASE COUNT          862 a         1585 c         1711 g          757 t
ORIGIN      
        1 gatggacgcc tccaacatgc tgaagccggc gctggcgcgg ggcgaattgc actgcgtcgg
       61 cgccacgacg cttgacgagt atcggaagca catcgagaag gatgcggcgc tggcgcggcg
      121 cttccagccg gtctttgtca acgagcccac ggtcgaggac acgatctcga tcctgcgcgg
      181 catcaaggag aaatacgagg tccatcacgg cgtgcgcatc accgacggcg ctatcgtcgc
      241 cgcggcgacg ctgtcgaacc ggtacatcac cgatcgcttc ctcccggaca aggccatcga
      301 cctcatggac gaggctgcgg cgcggctgcg catggcggcc gagtcgaagc cggaggcgat
      361 cgacgagctc gaccgtcgca tcatgcagct caagatcgag cgcgaggcgc tcaagaagga
      421 gagcgatgcc gcgtcgcgcg agcggctcga acggctcgaa gacgagctcg aagacctcga
      481 gaagaagtcg gccgagctca ccgcgcagtg gcgcgccgag aaggacaagc tcgccagcgc
      541 gcagaagctc aaggagcagc tcgaccaggc gcggatcgag ctcgagcagg tgcagcggcg
      601 cggcgactgg gcgcgtgccg gcgagctcac ttacggcgtg atccccgagc tcgagcgcaa
      661 gctcaaagag gccgaggcgg tcgagagcca tcgcatgatc gacgaggcgg taaccgccga
      721 gcacatcgcc ggtgtggtct cgcgctggac cggcatcccc gtcgacaaga tgctcgaggg
      781 cgagcgcgag aagctgctgc agatggagca gaatttaaag cgccgcgtca tcggccagga
      841 cgaggcggtg gtcgccgtct cgaacgccat ccgccgcgcg cgtgccggcc tccaggacgc
      901 gaaccgcccg atcggctcgt tcctgttcct ggggccgacc ggcgtcggca agaccgagct
      961 cgcccgcgcg ctcgccgaat tcatcttcgc cgatgagagc gcgatgatcc gcatcgacat
     1021 gtccgagtat atggagaagc actcggtcgc gcggctcatc ggcgcgcccc cgggctatgt
     1081 cggctatgag gaaggcggca gcctcaccga agccgtgcgt cgccggccct atcaggtgat
     1141 cctcttcgac gagatcgaga aggcgcatcc cgacgtcttc aacgtcttgc tgcaagtgct
     1201 cgacgacggg cggctcaccg acggccaggg ccgcaccgtc gatttccgca acaccctcat
     1261 cgtgctcacc tccaatctcg gcggcgagat cctggcgacg ttgccggaga atgtcgacgt
     1321 gtccgaggtg cgcaaccagg tgatggaggc cgtgcgcggc gcgttccggc cggagttcct
     1381 gaaccggctc gacgagatcc tgatcttccg gcgcctgtcg cgcgccgata tgaggggcat
     1441 cgtcgacatt cagctccggc ggctcgacaa gctgctggcc gaccgcaaga tcacgctcgc
     1501 ggtcgacgag aaggcgcgcg gctggcttgc ggaagcgggc tacgatcccg tctacggcgc
     1561 ccggcccctg aagcgggtga tccagcgctc gctgcagaat ccgctggccg agctgatcct
     1621 cggcggccgc atcagcgacg gcgagaccgt cgacgtgacg gtcggcgacg gcggcctcgc
     1681 catcgccggc cgcgtggcga aagcggcgga gtaacggcgg tgcgcgcccc tttgcggggc
     1741 ccgcaccatt tcggcatgat ctctgcataa tggtggccgc gacacaccac ctggaggcat
     1801 gccatgaccc gcgccgagct caccgagaaa atcgccgtca tcaagcgccg gaaaggcctc
     1861 tcctggcgcg acatcgccgc caagatcggc cccggctcgc cggtctacta cacggcggcg
     1921 ctgcacgggc agatgaagct cggcaaggcc gaggcggaag cggccggcaa gctgctcggc
     1981 ctcgacgagg atgaggtggc gctgctgcag gaagtgccct atcgcggctc attgccgacg
     2041 gcggtgccca ccgatccgct gatctaccgc ttctacgagc tgatccaggt ctacggcacc
     2101 acctggaagg cgctgatcca ggaggacttc ggcgacggca tcatgagcgc catcgatttc
     2161 gacatgaaga tcgagcgcca gccggaccag aaaggcgacc gcgtacgcgt cagcatgagc
     2221 ggcaagttcc tcccctacaa acaatactga gccgcgccgc ccgtctggcc atgtcgtctg
     2281 gctgttgccg gcgcgcgcgg gccggcgcat gatccgcgcc gacagttccg gggagaagag
     2341 cggtgctcga tggacaagta gcgtggatca ccggggccgg aagcggcatc ggccgagccg
     2401 gcgcgctcga gctggcggcg gccggcgctc gcgtcgcgct gagcgggcgg cgggccgagc
     2461 agctggaaca ggtcgcggcg gaaatcgcag caagcggcgg caaagccgag atgttcgccc
     2521 tcgatgtcgc cgaccgttcg gccgtcgccg cggcggcggc cgagattctc gaccgctgcg
     2581 gccctgtcga catcctcgtc aacagcgccg gcatcaatgt gccgaaccgc ttctggaaga
     2641 cgctgacgcc cgagagcttc gaccaggtcg tcgccgtcaa tctcaacggc gcgctctatt
     2701 gcacgcacgc cgtcctcccg gccatgcgcg cccggagaag cggcctcgtc atcaacatct
     2761 catcctgggc gggccgcttc gaatcctacc tcacgggccc cgcctacaat gccagcaagc
     2821 acgcgatggt ggcgatgacg ctctcgctca ccgacgagga atgcgtcaac ggcatccgcg
     2881 gctgcgtcat ctgcccgggc gaggtggcga cgccgatcct ccggcatcgg ccgaaaccgc
     2941 cgagtccgga agagatcgcg cgcatgcttc ggccggaaga tctcggccgc accatccgct
     3001 tcgtcgcgga gatgccggcc catgtctgcg tcaatgagat cgtgatcagc ccgacctgga
     3061 accgcttctt catgggcggc aaggacgtgg cgcgcggctg agccgcgctg gatcgattat
     3121 cgcggcggtc ccgtcacgtt cgccgcgacg acgggctcgc cccgcagcac ggggagtcgg
     3181 tggtcgatcg aagcggcgac ggtgcggaag gcggcgagat ccttgccggc gagcagcgga
     3241 cccggctgca tcttgatcga gagcggattg acggcatcgc catggatgcg cacctcgtag
     3301 tggagatgcg ggccggtcgc ccggccggtg gcgccgacat agccgatcac ctggccttga
     3361 tggatgcgct ggccggcatg ggtggcgagg cggctcatat gggcataggc cgtctcgaag
     3421 ctgttgctgt ggcgcagcag gacgaagttg ccgtagtcac ctttgtagcc agcggtctcg
     3481 accacgccgt cacccgcggc catcaccggc gtgcctggcg gtgacgcgaa gtcgacgccg
     3541 cgatgcatct ttgtgtagcc caggatcggg tgaaaccgca tgccgaagcc ggagctcagc
     3601 cgcgcgccgt cgaccggcgt gcgcagcaag gccttgcgga cgctctcgcc cttggcattg
     3661 aagaaatcgg cgcgggtgtc gcccgcgggc gtatagcgat agatgcgcag cgtattgccg
     3721 ctcaacgtca tcgacgcgta gatgatgttg tcggcgccga ccgccttgcc gctctcgtca
     3781 tagagccggt cgaagacgat ctcgaagctg tcgtcgggct gcacctcgcg ctggaaatcg
     3841 acgtcgtaac tgaaagcgtg gatcatctcg gcgaggatcg ccatgggcac gcccgcctcg
     3901 ctcgccgcat cgaagaggct ggagcggata acgccacggg caagcgcggg cgcgcgcacc
     3961 agcgggtgcg ctgcggcgtg agaggcgaaa tgcccgtcgc cggcgcgggt gacgatgacg
     4021 tcgcggtcga tggccggcgc aaggcgcagt tcctggagcc gcgccgcctg gaactgcaac
     4081 gccactttct ggccggtctt gagctcgcgc gggttccaga ccggcttgag cgcgtcgatg
     4141 acgtcctggg cgtcggccgc cgccacgcct tgatcgatga gaagacccat caaagtgtcg
     4201 cctgaggcga tctgcacgag atgggagata gcgggatccg ctgcggcggc ggccgggggc
     4261 tgcgcggtca tggcgggcgc tgccgcggcg gcggggggcg gagcgggtgc tgcgggccgc
     4321 gccgccgaga ggtcggcggc cggcgccatg gggaaattga ccaggaggaa gagagcggcg
     4381 acggcgacgg gaggacggag gaaagtcacg ggcagcctct cgcgatgcgg gtcatgcggt
     4441 gtttctcgat accctctgct caagcccacc catcgcagac atcgcagaga agattcgctt
     4501 tgtcaaccag ccgtttaccg tcgacgccgc gggtttcatc cgcggatatg catgacaccg
     4561 gaaggttaag acgggcctaa atcgccgctt tgacatacgg ggtgatgcct cccatacggg
     4621 atttaccgcc ctccgggcgg ctccttcatc agcgctggcg caagggtgca cctgccttca
     4681 ggcgggagcg gaatgttgcc gtcggttgcg gccgaatcat tagttgactt cggcgggacc
     4741 cgagcctata agcgcggctc cctcggcgag ggggtgctct ttgacattgt tggcaagagg
     4801 aagggatgcg caggcggcgg tgcctaatcg gtatcgcatg gtctgcgtat cttaggtaag
     4861 gaattgagca gtcaattccg agcgcgagat gagatcgtgc ggtgccagga cttgg
//
LOCUS       DASYNS010000471         3198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_429593, whole genome shotgun sequence.
ACCESSION   DASYNS010000471 DASYNS010000000
VERSION     DASYNS010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429593"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1272
                     /locus_tag="VGP50_14675"
     CDS             211..1272
                     /locus_tag="VGP50_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV8028676.1"
                     /translation="MSDKSEDSPFSVAHGAPDLPEVTVDSYNVELREGAAFVGDRASK
                     RAFNEILEEGRERLRRIDEDPLGAVPTEELSKKKLEKELASGDPEAAGLIQGAIEEFA
                     GEMAAVIRRFLRLKPWRGIERLVIGGGFAGSRIGELAIGRAAVLLKADGVELDLLPIR
                     HDPDEAGLLGAIHLAPAWIFSGHEGILAVDIGGSNIRAGIVAFDVKKRPDKARVGRSE
                     LWRHAEEKPTRDEAVDRLIAMLESLIGRATKEKLELAPFIGIGCPGVIRADGTIESGG
                     QNLPGNWESSRFNLPRLLREAIPDIGKAKTAVVMHNDAVVQGLSELPFMKDVKRWGAL
                     TIGTGLGNACFTNKTEAKR"
     gene            1362..1859
                     /locus_tag="VGP50_14680"
     CDS             1362..1859
                     /locus_tag="VGP50_14680"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8028677.1"
                     /translation="MAIVARPMPPEAALPPCDERIVELYRYWLSIHPARGVLPGRQHF
                     DPVMVPKLLPWIWLAEFEQNPLRFRYRLVGTEQVVALGRDATGKWFDEVHPGFAASAA
                     YRQFVAAVERSDVGFHKGAAINHRQRNWMSVERLVLPMARNGHDVDLLLGISVFMPAA
                     AAAAE"
     gene            complement(1828..2268)
                     /locus_tag="VGP50_14685"
     CDS             complement(1828..2268)
                     /locus_tag="VGP50_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HEV8028678.1"
                     /translation="MERQLLSILASTVLAIHLGVILFNIFGLVAIPLGAWRGWRFVRV
                     FWWRALHLAVLAVVALQALLDRACFLTLWQYALRRGAGEGASPAPLIESWVNRLIFWP
                     LPMWFFAALYVGVWIYALLLWRLVPPVLPGRTRRIIPRRRPPPA"
     gene            2316..2792
                     /locus_tag="VGP50_14690"
     CDS             2316..2792
                     /locus_tag="VGP50_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV8028679.1"
                     /translation="MAKQSAGVLLYRFRERRPEVFLVHPGGPFWAGKDAGAWSIPKGE
                     AGPGEDLLTRAKLEFAEETGFAVPDALQALRPIKQAGGKVVHAWAAEGNVDAAALRSN
                     GFTLEWPPRSGRQQIFPEVDRAAWFTLAEAREKINKGQSALLDQLEQLLAIRSNPD"
     gene            2794..>3198
                     /locus_tag="VGP50_14695"
     CDS             2794..>3198
                     /locus_tag="VGP50_14695"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEV8028680.1"
                     /translation="MPTRPRSLDAIDVKILAALQREGRSTIQKLADRVGLSARPCLER
                     VRRLEAAGIIVGYQAVLALEKLAKPITVFAEIALESHGRQDQFERRLAAIEEVVECWE
                     VSGAFDYLARVVCADVARYETLTADLIDDPKLG"
BASE COUNT          584 a         1035 c         1075 g          504 t
ORIGIN      
        1 cgcaggcgat tcgctgatga tcctcgaatc gatgaagatg gagatacccg ttctcgcgcc
       61 cgccgccggc aaggtgttgc gtctgatggt cggggaggcg gatccggtga gcgagggcca
      121 gctcctggcc gtcatcgaag cataaagcga gcctgtggac cttccgcggc ctgcccgccg
      181 ttcaatgagg gcggccactc gtggagttcc atgtcggata agtccgaaga ctcgcccttt
      241 tccgtcgccc atggggcgcc cgatctgccg gaagtgacgg tcgacagcta caatgtcgag
      301 ctgcgcgagg gcgcggcctt tgtcggcgac cgcgccagca agcgcgcctt caacgagatt
      361 ctcgaggaag ggcgcgagcg cctgcggcgc atcgacgagg atccgcttgg cgcggtgccg
      421 acggaagagc tcagcaagaa gaagctcgag aaggagctgg cgtcgggcga tcccgaagcg
      481 gccggcctca tccaaggcgc catcgaggaa ttcgccggcg agatggccgc ggtgatccgg
      541 cgatttcttc ggttgaagcc gtggcgtggc atcgagcgac tcgtcatcgg cggcggattc
      601 gccggcagcc gcatcggcga gctggccatc ggccgcgccg cggtcctcct caaggccgat
      661 ggcgtggagc tcgatctgct ccccatccgt cacgaccccg acgaagccgg gctgcttggc
      721 gccattcatc tagcgccggc ttggatcttc tcgggccatg agggcattct tgcggtcgat
      781 atcggcggct ccaacatccg cgccggcatc gtcgcgttcg atgtcaagaa gcgtcccgac
      841 aaggcgcgtg tcggccgctc ggagctctgg cgccacgccg aggaaaagcc gacgcgcgat
      901 gaagccgtcg accgcctgat cgcgatgctc gaatcgctga tcgggcgcgc gacgaaggag
      961 aagctggagc ttgcgccttt catcggcatc ggctgccccg gcgtcattcg cgccgacggg
     1021 acgatcgaaa gcggcggaca gaatctgccg gggaactggg agagcagccg cttcaatttg
     1081 ccgaggcttc tgcgcgaggc gattccggac atcggcaagg cgaagacggc ggtcgtcatg
     1141 cacaacgacg ccgtcgtgca gggcttgagc gagctgccct ttatgaagga cgtgaagcgc
     1201 tggggcgcgc tcacgatcgg aaccggcctc ggcaacgcct gcttcaccaa taaaaccgaa
     1261 gcaaagcgct aagcgagcca caacgataag ttgcatccgc tccagtcgcc gccgcggcgc
     1321 cgtcaagaaa acatcaatgg tatgcggata agctccggtc catggcgatc gttgcccgcc
     1381 ccatgccacc tgaagccgca ttgccgccct gcgacgagcg gatcgtcgaa ctctaccgct
     1441 attggctgtc gatacacccg gcgcgcggcg ttctgccggg gaggcaacat ttcgacccgg
     1501 tgatggtccc gaagcttctg ccctggatct ggctcgccga attcgagcaa aatcccctgc
     1561 gcttccgcta tcgcctggtg ggcaccgagc aagtggtggc actgggccgc gatgccaccg
     1621 gcaaatggtt cgatgaagtt catcccggct tcgctgcgtc ggccgcttat cgccaattcg
     1681 tcgctgccgt cgagcgcagc gacgtcggct tccacaaggg cgcggccatc aaccaccgcc
     1741 agcgcaactg gatgtcggtc gagcgcctgg ttctgccgat ggcgcgcaac ggccatgacg
     1801 tcgacctgtt gctgggcatc agcgtcttca tgccggcggc ggccgccgcc gcggaatgat
     1861 ccggcgcgtc cggccgggca gcaccggcgg tacgaggcgc cacaacagca gcgcatagat
     1921 ccagacgccg acatagagcg ccgcgaagaa ccacatcggt agcggccaga agatcaggcg
     1981 attgacccag ctttcgatga gcggggccgg cgatgccccc tccccggcac cccgccgcaa
     2041 tgcgtattgc cagagcgtca ggaagcacgc gcgatcgagc agcgcttgca gcgcgacgac
     2101 cgcgagaacg gcgagatgaa gcgcgcgcca ccagaagacg cggacgaaac gccaaccgcg
     2161 ccaggcaccc agcggtatcg cgacgaggcc gaagatattg aacaggatca cgccgagatg
     2221 aatggccagc accgtgctcg ccaatatcga aagcagctga cgctccatgg cggctgaccg
     2281 ttgacatccc gaaaggccgc ggctctgttg gagccatggc gaagcaaagc gctggtgtgc
     2341 tgttatatcg ctttcgagag cgtcgtccgg aagtgttcct cgttcacccg ggaggaccgt
     2401 tctgggccgg caaggatgcg ggcgcctggt cgattcccaa gggcgaagca gggccggggg
     2461 aggatctgtt gacacgcgcg aagctcgaat ttgccgaaga aacggggttc gccgtgcccg
     2521 atgccttgca ggcgctgcgc ccgatcaagc aggccggcgg caaggtcgtg cacgcctggg
     2581 cggccgaagg caatgttgat gcggcggcgc tccgcagcaa cggcttcacc ctcgaatggc
     2641 cgccgcgctc gggccgccag cagatctttc cggaggtcga ccgcgcggcg tggttcacgc
     2701 ttgccgaggc acgcgagaag atcaacaaag gccagagcgc tctcctcgat cagctcgagc
     2761 agctcctcgc catccgctcg aacccggact gacatgccga cgcgcccgcg cagcctcgat
     2821 gcgatcgacg tcaagatcct ggcggcgctg cagcgcgaag ggcgcagcac catccagaaa
     2881 ttggcggacc gcgtcgggct ttcggcgcgg ccctgtcttg agcgggtgcg ccggctcgag
     2941 gcggccggca tcatcgtcgg ctaccaggcg gtgctggcgc tcgagaagct ggcgaagccg
     3001 atcaccgtgt ttgccgagat cgcgctcgag agccatgggc ggcaggatca gttcgagcgc
     3061 cgtctcgcgg ccatcgagga ggtcgtcgaa tgctgggagg tgagcggcgc cttcgactat
     3121 ctggcgcggg tcgtctgcgc ggacgtcgcc cgctacgaga cgctcaccgc cgacctcatc
     3181 gacgacccga agctcggc
//
LOCUS       DASYNS010000472         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3862691, whole genome shotgun sequence.
ACCESSION   DASYNS010000472 DASYNS010000000
VERSION     DASYNS010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3862691"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="VGP50_14700"
     CDS             complement(<1..1156)
                     /locus_tag="VGP50_14700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV8028681.1"
                     /translation="MKFGIFYEHQIPRPWQDGDEHRTYQQALDQVELADRIGIDHAWE
                     VEHHFLEEYSHSSAPEVFLAACSQRTKQIRLGHGIVLMPPNYNHPARVAERVAALDLV
                     SNGRVEFGTGESSSILELGGFGVNPDEKRAMWAEALEQCLNMMVMTPYPGFAGKYFSM
                     PCRNIVPKPLQRPHPPVWVACSNRETIKLAARLGIGALTFAFVDPAEARQWVEDYYRI
                     FKEECTPIGHAVNPNVAMITGFSVHRDRAEAARRGEDGFRFFGYALAHHYIFGTHRPG
                     LTDIWANFEKARPALPPAGANRGIGTPDDLREHLRGFEAAGVDQVAFIQQGGRNRHEH
                     ICEALELFARDVMPEFKEREAARQAKKARELAPYIERAMARKQRMPALDDD"
     gene            complement(1182..2210)
                     /locus_tag="VGP50_14705"
     CDS             complement(1182..2210)
                     /locus_tag="VGP50_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028682.1"
                     /translation="MTQLVYSEAELFAEHAYARPQIEAGYRLHGGFDREGRYQSPRTL
                     LRWPAIQAWQAELERRGFPLIDATTRLLQRRAYPGFEQQKMLLDLGLGQTLWNALTVT
                     GIMEARGHFLAEFIAPEFRTIVADDLAGTATGHLGKGLLKAHGFDEGGRPERGEGGHD
                     AMWFAVRDLLFGRDAYPLPEIPESLARPEFDRLMPQIPPAHEKLLMMLMNVLMIEVRA
                     EAFFSFCIHLMRASELFRDRRADAEHAAALVERIRQDEAIHVAYLQTVVSELRSFSFR
                     TTDGDRVRGERFIDPIWSGLVHWHAVTRADHAREQTRATIEALLLAAPDGTRRLAAFD
                     ALEQREAA"
     gene            complement(2207..2878)
                     /locus_tag="VGP50_14710"
     CDS             complement(2207..2878)
                     /locus_tag="VGP50_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8028683.1"
                     /translation="MLQVPDGTVIADRAERRKAESRERLLEAARRLFIERGYHATRPQ
                     DIARAAGVGHGTFYLHFADKQECFLAFAARACDELQAFVDARLAGVEGVEPQLRALLE
                     ALLDYAEQNPGEIKAAMTDVSVIASDGVVHESVIDRWATQWAERLRLGAAAGAIHADY
                     DAAVAGHAIVGLIRGAAAYGRRHRSARAQVVDTVARFLARALVSESEAEAARHHRLLR
                     GRLES"
     gene            complement(3059..>3354)
                     /locus_tag="VGP50_14715"
     CDS             complement(3059..>3354)
                     /locus_tag="VGP50_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028684.1"
                     /translation="CLWGLFIVLRAIWHLNLLGWAPLWWLTYIAGGALLAGLGFVTGY
                     PLLSQYILSKNPEVAKQSEVALAKLTPYQTTLGYAGIVFAIWMLIVRISGGVG"
BASE COUNT          568 a         1082 c         1166 g          538 t
ORIGIN      
        1 cgtcgtcgtc gagcgccggc atgcgctgct tccgcgccat ggcgcgctcg atataaggtg
       61 cgagctcgcg cgcctttttc gcctggcgcg ctgcttcgcg ctctttgaac tccggcatga
      121 cgtcgcgggc gaacagctcg agcgcctcgc agatatgctc atggcggttg cgcccgcctt
      181 gctggatgaa ggcgacctgg tcgacgccgg cggcctcgaa gccgcgcaga tgctcgcgca
      241 ggtcgtcggg cgtcccgatg ccgcggttgg cgcccgccgg cggcagggca gggcgtgctt
      301 tctcgaaatt cgcccagatg tcggtgaggc cgggccggtg cgtgccgaag atatagtgat
      361 gcgccagcgc atagccgaag aagcggaagc cgtcctcgcc gcggcgcgcg gcctccgcgc
      421 ggtcgcgatg caccgagaag cccgtgatca tggcgacatt gggattgacc gcatggccga
      481 tcggcgtgca ttcctccttg aagatgcggt agtagtcctc gacccattgc cgggcttccg
      541 ccggatcgac gaaggcgaag gtcaaggcgc cgataccgag ccgcgcagcg agcttgatgg
      601 tctcgcggtt ggagcacgcc acccacaccg gcgggtgcgg ccgctgcagc ggcttcggca
      661 cgatgttgcg gcagggcatc gagaaatatt tgcccgcgaa gccgggataa ggcgtcatca
      721 ccatcatgtt gaggcattgc tcgagagcct cggcccacat cgcgcgcttt tcgtcggggt
      781 tcacgccgaa gccgccgagc tccaagatcg acgaggattc gccggtgccg aactcgacgc
      841 ggccattcga gacgagatcg agcgcggcga cgcgctcggc gacccgggcc gggtgattgt
      901 agttgggcgg catcagcacg atgccatggc cgagccggat ctgtttcgtg cgctgcgagc
      961 aggcagcgag gaagacctcc ggcgccgagg aatgcgagta ttcctcgagg aaatgatgct
     1021 cgacctccca ggcatggtcg ataccgatgc ggtcggcgag ctcgacctgg tcgagcgcct
     1081 gctgataggt ccgatgctcg tcgccgtcct gccagggccg cgggatctgg tgctcgtaga
     1141 agatgccgaa tttcacccgg cgcggctctc ggcgggcccg gtcatgcggc ttcgcgctgc
     1201 tccagcgcgt cgaaggcggc gagccgcctc gtgccgtcgg gagcggcgag aagcagcgcc
     1261 tcgatggtgg cgcgcgtctg ctcccgcgca tgatcggcgc gggtcacggc gtgccagtgc
     1321 acgaggccgc tccagatcgg atcgatgaag cgctcgccgc gcacgcggtc gccatccgtg
     1381 gtgcggaagc tgaagctgcg cagctccgac acgaccgtct gcagataggc gacgtggata
     1441 gcctcgtcct ggcggatacg ctcgaccaga gccgcggcat gctccgcgtc tgcgcggcgg
     1501 tcgcggaaca gctcgctcgc gcgcatgagg tgaatgcaga agctgaagaa ggcctcggcg
     1561 cgcacttcga tcatcagcac attcatgagc atcatcagga gcttctcatg ggccggcggg
     1621 atctgcggca tgagccggtc gaactcgggc cgcgcgaggc tttccggaat ttccggaaga
     1681 ggataggcgt ccctgccgaa gagcaggtcg cgcaccgcga accacatggc gtcatggccg
     1741 ccttcgcccc tctcggggcg gccgccttca tcgaagccat gggccttcaa gagccccttg
     1801 ccgagatggc cggtcgcggt gccggcgaga tcgtccgcga cgatggtccg gaattcggga
     1861 gcgatgaatt cggcaagaaa atggccgcgc gcttccatga tgccggtgac cgtgagcgcg
     1921 ttccacagcg tctggcccag gccgagatcg agcagcatct tctgttgctc gaagcccgga
     1981 taggcgcgcc tttgcaagag ccgcgtcgtc gcgtcgatca gcgggaagcc gcggcgctcg
     2041 agctcggcct gccaggcctg gatggcgggc cagcggagga gcgtgcgcgg cgactgatac
     2101 cggccctccc gatcgaagcc gccatggaga cgatagccgg cctcgatctg cggccgggca
     2161 taggcgtgct cggcgaagag ctcggcctcg ctgtagacga gctgggtcat gattccaacc
     2221 tcccgcggag caagcgatgg tgccgcgccg cctcggcttc ggactcggat acgagcgccc
     2281 gcgcgaggaa gcgcgcgacg gtgtcgacga cctgcgcgcg tgccgagcga tggcggcggc
     2341 cgtaagcggc ggcaccgcga atgaggccga cgatggcatg gcccgcgacc gcggcgtcgt
     2401 agtcggcatg gatcgcgccg gcagcggcgc cgaggcgcag gcgctcggcc cattgcgtcg
     2461 cccagcgatc gatcaccgac tcatgcacga cgccgtcgct ggcgatcacc gacacatcgg
     2521 tcatggccgc tttgatctcg ccgggattct gctcggcata gtcgaggagc gcctccagca
     2581 gagcgcgaag ctgcggctcg acgccctcga cgcccgcaag gcgcgcatcg acgaaggcct
     2641 ggagctcgtc acaggcgcgc gcggcgaagg cgaggaagca ttcctgcttg tccgcgaaat
     2701 gcagatagaa ggtgccatgg ccgacacccg ccgcccgcgc gatgtcctgc ggccgggtgg
     2761 cgtggtatcc ccgctcgatg aagagccggc gggccgcttc gaggagccgc tcccggctct
     2821 cggctttccg ccgttcggcg cggtcggcaa tgacagtccc gtcgggcacc tggagcatca
     2881 attccgtctt cttagtttcg ccttcatgta ctgattattt gtcagtggtg acacgacgtc
     2941 aatgaccgtc gcgggcggcg cgccggagaa agcgtcagaa ttctaagccg ggcggagaag
     3001 accgcgtgtg gcgcaagtca catcacagac gggcgaagac ctcgcccggc ggttcgcgtc
     3061 agccgacgcc gccgctgatc ctgacgatca gcatccagat ggcaaagacg atcccggcat
     3121 agccgagagt ggtctggtag ggggtgagct tggcgagcgc gacctcgctt tgcttcgcga
     3181 cttcagggtt cttgctgagg atatattgcg acagcaacgg atatcccgtg acaaagccga
     3241 gcccggccag cagcgcaccc ccggcgatat aagtgagcca ccacagcggc gcccagccca
     3301 gcaagttcag atgccagatc gcgcggagca cgatgaagag gccccaaaga caac
//
LOCUS       DASYNS010000473         3064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3084528, whole genome shotgun sequence.
ACCESSION   DASYNS010000473 DASYNS010000000
VERSION     DASYNS010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3084528"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VGP50_14720"
     CDS             <1..534
                     /locus_tag="VGP50_14720"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase light chain EutC"
                     /protein_id="HEV8028685.1"
                     /translation="ERHTYLLRPDLGRRLDDTSRESLSTLPRGHDLVFVLADGLSARA
                     LMRHALPLLGAVLPLFRSHAWRVGPTAVVEQGRVAIGDEIGAVLDAVLVAMLIGERPG
                     LSAPDSLGVYLTWAPKPGRSDAERNCLSNIRPDGMAYAEAAQRLFHLATEARRRQITG
                     VALKDETMPTLPSGDGD"
     gene            complement(577..2223)
                     /locus_tag="VGP50_14725"
     CDS             complement(577..2223)
                     /locus_tag="VGP50_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEV8028686.1"
                     /translation="MNAAIARAERIVPMTGVSREVEAPPVPANTNMPAEVSFKTWLAV
                     VGATLGAFLAVLNIQIVNSSLADIQGAIGAGIDDGGWVSTSYLVAEIITIPLSGWLAS
                     VFSLRRYLLANTALFLLFSASCAFAENLGQMIVLRAFQGFTGGVLIPLAFTIIMTKLP
                     KAKQPVGMALFALSATFAPAIGPTIGGYLNDNYGWQFVFYVNLAPGVVMLAMLWASLE
                     REKMRLALLRQGDWAGIVTMAVGLGALQTMLEEGNKDDWFGSPFILRLGVVAVIGLAL
                     FLWIELRTAKPLLNLRLLRRRNFGFGSIANFLLGGALYGSSFVLPLYLSQMQGYDAEQ
                     IGIVLAWTGLPQLLLIPLVPRLMRRIDARRLAVLGLALFALSNFMNGWLTQDVAGDQL
                     FWPNIVRAVGQALVMTPLSAIAVAGIEAQNTASASALFNMMRNLGGAIAIAALQTFVT
                     KREQFHSNVLTSSVSLFGEATRTRLAELSQYFMAHGLADPGTAGHEAMVAIGRAVRIQ
                     ASVMAYGDTFLLLGAGLALAVLATLGLRRSDASGAGGAAH"
     gene            complement(2236..>3064)
                     /locus_tag="VGP50_14730"
     CDS             complement(2236..>3064)
                     /locus_tag="VGP50_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV8028687.1"
                     /translation="RDLRTALDQAAADVATGAAEIGNIDAQLQLQHSLIEEARAAILA
                     DQAAVTFASEDAQRQHDLMQREVTSVRVAQQSQTALRQQAAALQRDQAALGAAEQQIE
                     VLKTARAKAEAQLQHFQAIEAQARLNLGYATITAPVDGTVGARSLRVGQYVQAGTALM
                     AVVPLKAVYVVGNFKETQLTDVRPGQPVEIRIDTFPGARLNGHVDSLAPASGLTFALL
                     PPDNATGNFTKIVQRIPVKIALDDGALAGLLRPGMSVEPTIDTQAGLPTAAPRVAAR"
BASE COUNT          510 a         1036 c         1060 g          458 t
ORIGIN      
        1 gagcgccaca cctatctgct tcgtcccgat ctcggccggc ggctcgacga cacctcgcgc
       61 gagagcctta gcacactgcc gcgcggtcac gacctcgtct tcgtgctggc cgacgggctg
      121 tcggcgcgcg cgctcatgcg ccatgcgctg ccgctgctcg gcgccgtgct gccgttgttc
      181 cgcagccatg cctggcgcgt cggccccacc gcggtggtcg aacagggccg cgtcgccatc
      241 ggcgacgaga tcggcgcggt gctcgacgcg gtgctggtcg ccatgctcat cggcgagcgt
      301 ccgggcctga gcgcgcccga cagtctcggc gtctatctca cctgggcgcc caagcccggc
      361 cgcagcgacg ccgagcgcaa ctgcctctcg aacatccgcc ccgacggcat ggcctacgcc
      421 gaggccgcgc agcgcctctt ccatctcgcg acggaagcgc ggcgccgtca gatcaccggc
      481 gtcgccctca aggacgagac gatgccgacg ctgccttccg gcgacggcga ctgaagaaaa
      541 gattcaccac tgaggcgatg accctttctt tctgtgctaa tgcgccgcgc cgcctgcgcc
      601 ggaagcgtcg ctccgccgca atcccagcgt cgccaggacg gcgagcgcga ggccggcgcc
      661 gagcagcagg aaggtgtcgc cataggccat gaccgaggcc tggatgcgga ccgcgcggcc
      721 gatcgccacc atggcttcat gcccggcggt gccagggtcg gcgaggccat gcgccatgaa
      781 atactggctg agctcggcaa gccgtgtgcg cgtcgcctcg ccgaagagcg agaccgacga
      841 ggtcaacaca ttcgagtgga actgctcgcg tttcgtgacg aaggtctgca gcgccgcgat
      901 ggcgatggcg ccgccgagat tgcgcatcat gttgaagagc gcggacgccg atgcggtgtt
      961 ctgcgcctcg atgccggcga cggcaatcgc cgaaagcggc gtcatgacca gggcttgccc
     1021 cacggcgcgc acgatattgg gccagaagag ctggtcgcct gccacgtcct gcgtcagcca
     1081 gccattcatg aagttgctga gagcgaagag ggcgaggccg aggacggcca gccgccgcgc
     1141 gtcgatacgc cgcatcaggc gcggcaccag gggaatgagc agaagctgcg gcaagcccgt
     1201 ccaggccagc acgatgccga tctgctcggc atcgtagccc tgcatctgcg agaggtagag
     1261 cggcagcacg aaggacgagc cgtaaagcgc gccgcccagg aggaagttgg cgatgctgcc
     1321 gaagccgaaa ttgcgccgcc gcaacaggcg cagattcaac aatggctttg ctgtccggag
     1381 ctcgatccac aggaaaagcg cgagcccgat gacggcgacc acgccgagcc gcaggatgaa
     1441 gggcgagccg aaccagtcgt ccttgttccc ttcctcgagc atggtctgca atgcgccgag
     1501 gccgaccgcc atggtgacga tgccggccca gtcgccctgc ctgagcagcg caagacgcat
     1561 cttctcgcgc tcgagcgacg cccacagcat ggcgagcatc acgacacccg gcgcgagatt
     1621 gacatagaag acgaactgcc acccgtaatt gtcgttgaga tagccgccga tggtgggccc
     1681 gatcgccggc gcgaaggtcg cggagagcgc gaagagcgcc atgccgacgg gctgcttcgc
     1741 cttcggcagc ttggtcatga tgatggtgaa ggcgagcggg atcaggacgc cgccggtgaa
     1801 gccctggaac gcgcgcagca cgatcatctg cccgagattc tcggcaaagg cgcagctcgc
     1861 cgagaagaga aggaagagcg ccgtgttggc caggagatag cggcgcagcg aaaatacgct
     1921 cgcgagccag ccgctcagcg ggatcgtgat gatctcggcc acgagatagg acgtcgagac
     1981 ccagccgccg tcatcgatgc cggcgccgat tgcgccttgg atgtcggcca gcgaggagtt
     2041 gacgatctga atgttgagca ccgcgaggaa agcgccgagc gtcgcgccga cgacggcgag
     2101 ccaggtcttg aaggacacct cggcgggcat gttcgtgttg gcgggaaccg gcggcgcctc
     2161 gacctcccgc gaaactccgg tcatcgggac gatgcgctcg gcccgggcga tggcggcatt
     2221 catcttccgc cctcctcacc gcgccgcgac gcgcggcgct gccgtcggca ggcccgcctg
     2281 ggtgtcgatg gtcggctcga ccgacatgcc ggggcgcagg aggccggcca gcgcgccgtc
     2341 atcgagcgcg atcttcacgg ggatgcgctg cacgatcttg gtgaaattgc ccgtcgcgtt
     2401 gtccggcggc agcagcgcga aggtgaggcc gctcgccggc gccaggctgt cgacatggcc
     2461 gttgagccgc gcgccgggaa acgtatcgat gcggatctcg accggctgcc ccggccggac
     2521 atcggtgagc tgcgtctcct tgaagttgcc gacgacatag acggctttga gcggcacgac
     2581 ggccatgagg gccgtgcccg cctgcacata ttgtccgacg cgcaggctgc gcgcgccgac
     2641 cgtgccgtcg acgggggccg tgatcgtggc atagccgaga ttgagccgcg cctgcgcctc
     2701 gatcgcctgg aaatgctgga gctgcgcctc ggctttggcg cgcgcggtct tcagcacctc
     2761 gatctgctgc tcggccgcgc ccagcgccgc ctggtcgcgc tgcagcgccg ccgcctgctg
     2821 gcgaagcgcc gtctggctct gctgcgccac gcgcaccgag gtcacctccc gctgcatgag
     2881 gtcgtgctgg cgttgcgcgt cttcgctggc gaaggtgacc gcagcctgat cggcgaggat
     2941 ggcggcgcgc gcctcttcga tcagcgaatg ttgcagctgc agctgcgcat cgatattgcc
     3001 gatctccgcc gcgccggtcg cgacatcggc cgccgcctgg tcgagcgcgg tgcgcaagtc
     3061 gcgg
//
LOCUS       DASYNS010000474         3861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4331457, whole genome shotgun sequence.
ACCESSION   DASYNS010000474 DASYNS010000000
VERSION     DASYNS010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4331457"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /gene="thrC"
                     /locus_tag="VGP50_14735"
     CDS             <1..1083
                     /gene="thrC"
                     /locus_tag="VGP50_14735"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV8028688.1"
                     /translation="LAFKDYALQLVGPLFDHVLAKRGTRVTIIGATSGDTGSAAIEAC
                     RDRAALDVFILYPKDRLSEVQRRQMTTVASPNVHAIAIAGNFDDCQDLVKAMFADQAF
                     RAAFNLSAVNSINWARIMAQVVYYVVTAVALGAPDRAIGFAVPTGNFGNVYAAHVARR
                     MGLPIAELVIGTNRNDILARFLNDGAMTIRAVEPSLSPSMDIQISSNFERLLFELKDR
                     QGAAVADAMATFRRTGTLPTSTAEVQAARRLFSAERVDDAATRAQIAATWRSTGELID
                     PHSAIGVAAAERGRRQRAVPMVALASAHPAKFPDAVEQATGQRPQLPRRLGDLMTRPE
                     RITVLANDLRAVQNFIRAQARAESPA"
     gene            1080..2339
                     /locus_tag="VGP50_14740"
     CDS             1080..2339
                     /locus_tag="VGP50_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV8028689.1"
                     /translation="MTLRLTTLENGMRVITDAMDGVETVSLGVWVDVGTRHEPAEING
                     VAHLLEHMAFKGTERRSALDIASEIEAVGGHLNAYTSREHTAYYAKVLKEDTPLAVDI
                     LADILQHSVFDQRELERERTVILQEIGQANDTPDDVIFDLFQERAFPDQAMGRPVLGR
                     SEVISRMERDTVAQYLHGHYAAPGMLLAAAGNLDHDVLVAQARSAFAALPQQRVARDE
                     AARYVGGDLREERDLEQVHLVLGFPGFPYGDPDYYAASVVSAALGGGMSSRLFQEIRE
                     KRGLVYAIYSFTHTYSDTGLFGVYAGTGEAEVAELMPVLCEEIRRLGGGFEAAELQRA
                     RAQLKAGLLMSLESTTARCEQQAGHMLVFGEPLNVADVVARIDAVDDAAVGRVARRLL
                     TAPPTLTALGPIGKIEPYPRIAERLGG"
     gene            2354..2965
                     /locus_tag="VGP50_14745"
     CDS             2354..2965
                     /locus_tag="VGP50_14745"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV8028690.1"
                     /translation="MASDILRLPRLFATAPPALRLVGERVLLRPPERGDWQEWAELRG
                     ESRRFLAPWEPTWSADSLTRGAFRRRLQRYGADWRSDQGYSFFLFRVGDGAMVGGIGL
                     SNVRRGVAETGSLGYWTGERHARRGYMMAGLELILGFSFNRLKLHRVEAACLPHNGPS
                     RALLLKSGFREEGYAKEYLCIDGRWQDHVLFAMLRDEWAGKGG"
     gene            3000..3470
                     /locus_tag="VGP50_14750"
     CDS             3000..3470
                     /locus_tag="VGP50_14750"
                     /inference="COORDINATES: protein motif:HMM:NF037873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028691.1"
                     /translation="MPDDLRRIYEAEGRAHFYFDDKVPRDLFRGQTRTEEKQGLPILY
                     PHPGYKKKDGSERLPDVLVIERDGQQIVKGCRCTQGDYRGVSTFDQKSGNLRGARWYR
                     LPKSTAIPEALAITQDTDRTDRPNHFTIAPKDDMPLPLFQVWLNALARRMTEDK"
     gene            3470..3694
                     /locus_tag="VGP50_14755"
     CDS             3470..3694
                     /locus_tag="VGP50_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028692.1"
                     /translation="MVEISITNLVLAIIAMDRDIKAREKALRDGLISEANEDEETQYV
                     LDLWKARSELIGAHDRARERDPKLSPIKLD"
BASE COUNT          621 a         1337 c         1295 g          608 t
ORIGIN      
        1 ctcgctttca aggactatgc gctgcagctc gtggggccgc tcttcgatca cgtgctggcc
       61 aagcgcggca cgcgcgtcac gatcatcggc gcgacctcgg gcgataccgg gtcggcggcg
      121 atcgaggcct gccgcgaccg cgccgcgctc gacgtcttta tcctctatcc gaaggaccgc
      181 ctctccgagg tgcagcggcg gcagatgacg acggtcgcct cgcccaacgt ccatgccatc
      241 gccatcgccg gcaatttcga cgactgccag gacctggtga aggcgatgtt cgccgatcag
      301 gcgttccgcg ccgccttcaa cctctcggcg gtgaactcga tcaactgggc gcggatcatg
      361 gcgcaggtgg tctattacgt ggtcacggcc gtcgctctgg gcgcccccga ccgcgcgatc
      421 ggcttcgccg tgcccaccgg caatttcggc aatgtctacg ccgcccatgt ggcgcggcgc
      481 atggggctgc cgatcgccga gctggtcatc ggcaccaacc gcaacgacat cctggcgcgc
      541 ttcctcaatg acggcgccat gaccatccgc gccgtcgagc cgagcctgag cccgagcatg
      601 gacatccaga tctccagcaa tttcgagcgc ctgctcttcg agctgaagga ccggcagggc
      661 gccgccgtcg ccgacgccat ggccacgttc cgccgcaccg gcacgctccc gaccagcacg
      721 gcggaagtgc aggcggcgcg ccggctcttc tcggccgagc gcgtcgatga cgccgccacg
      781 cgcgcgcaga tcgcggcgac ctggcgcagc accggcgagc tcatcgatcc acacagcgcc
      841 atcggcgtcg ccgcggccga gcgcggccgg cggcagcgcg cggtgccgat ggtggcgctc
      901 gcctcggcgc atccggcgaa gtttcccgac gccgtcgagc aggcgaccgg gcagcggccg
      961 caactgccgc gccgcctcgg cgatctcatg acgcggccgg agcggatcac cgtcctcgcc
     1021 aacgacctgc gcgcggtcca gaatttcatc cgcgcccagg cgcgagcgga gagcccggca
     1081 tgaccttgcg cctgaccacc ctcgagaacg gcatgcgcgt catcaccgac gccatggacg
     1141 gcgtcgagac ggtgtcgctc ggcgtctggg tcgatgtcgg cacgcgccac gagcccgccg
     1201 agatcaacgg cgtcgcccat ctcctggagc acatggcctt caagggcacc gagcgccgct
     1261 ccgctctcga catcgccagc gagatcgagg cggtgggcgg ccatctcaat gcctatacct
     1321 cgcgcgagca cacggcttat tatgccaagg tgctcaagga ggacacgccg cttgccgtcg
     1381 atatcctggc cgacatcctg cagcattcgg tgttcgacca gcgcgagctc gagcgcgagc
     1441 gcacggtgat cctgcaggag atcggccagg ccaatgacac gcccgacgac gtcatcttcg
     1501 atctcttcca ggagcgcgcc ttccccgacc aggcgatggg acggccggtg ctcggccgta
     1561 gcgaggtcat cagccgcatg gagcgcgaca cggtcgcgca atacctgcac ggacattacg
     1621 cggcgccggg catgctgctc gccgccgccg gcaatctcga ccacgacgtg ctggtggcgc
     1681 aggcgcgaag cgccttcgcc gcgctgccgc agcagcgcgt cgcgcgcgac gaggcggcgc
     1741 gctatgtcgg cggcgacctg cgcgaggagc gcgacctcga gcaggtccat ctcgtgctgg
     1801 gcttcccggg gttcccttat ggcgatcccg actattatgc tgcctcggtg gtctcggcgg
     1861 cgctgggcgg cggcatgtcc tcgcgcctgt tccaggagat ccgcgagaag cgcggcctcg
     1921 tctatgccat ctactccttc acgcacacct atagcgacac cggcctcttc ggcgtctatg
     1981 ccggcactgg cgaggcggaa gtggcggagc tgatgccggt gctgtgcgag gagatccgcc
     2041 gcctcggcgg cggcttcgag gccgccgagc tgcagcgcgc ccgcgcccag ctcaaggccg
     2101 ggctcctcat gtcgctcgaa agcacgaccg cgcgctgcga gcagcaggcg gggcacatgc
     2161 tggtgttcgg cgagccgctc aatgtcgcgg acgtggtggc gcgcatcgac gccgtcgacg
     2221 acgcggccgt ggggcgggtg gcgcgccggc tgctcacggc gccgccgacg ctgacggcgc
     2281 tcgggccgat cggcaagatc gagccctatc cccgcatcgc cgagcgcctc ggcgggtgag
     2341 agggtggggc gctgtggctt ccgacatcct ccgattgccg cggctcttcg ccaccgctcc
     2401 gccggcgctg cggctcgtcg gcgagcgcgt cctgctgcgc ccgcccgagc gcggcgactg
     2461 gcaggaatgg gcggagctgc gcggcgagtc gcgccgcttc ctcgccccct gggagccgac
     2521 ctggtcggcg gattcgctga cgcgcggcgc cttccgccgc cgcctccagc gctacggcgc
     2581 cgattggcgc agcgaccagg gctatagctt cttcctgttc cgcgtcgggg acggcgcgat
     2641 ggtcggcggc atcggcctca gcaatgtccg ccgcggcgtc gccgagaccg gcagcctcgg
     2701 ttactggacc ggcgagcgcc atgcgcggcg cggctatatg atggcgggcc tcgagctgat
     2761 cctcggcttc tccttcaacc gcctgaagct gcaccgcgtc gaggcggcgt gcctgcctca
     2821 taacggaccg agccgcgcgc tcctcctcaa gagcggcttc cgcgaggaag gctacgccaa
     2881 ggaatatctc tgcatcgacg gccgctggca ggaccacgtc ctcttcgcca tgctgcgcga
     2941 cgagtgggcg ggaaagggcg gctgagccgg ttgcctggat agcaccttgg gggaatgatg
     3001 tgccggacga tctcagacga atctacgagg cggagggccg cgcccacttc tatttcgacg
     3061 acaaggtgcc gcgcgatctc tttcgcggcc agactcggac agaggagaaa caggggctcc
     3121 cgatcctcta tccgcatcct ggctacaaga agaaagacgg cagcgaacgc ctccctgatg
     3181 tgctggtgat cgagcgagac gggcaacaga tcgtcaaagg ctgccgttgc acccaggggg
     3241 attatcgggg cgtatccacg ttcgaccaga agagcgggaa cctacgcggt gcccgctggt
     3301 acaggctgcc gaagtcgacg gcgatccccg aggcgctcgc gatcacgcag gatacggata
     3361 ggacggatcg cccgaaccac ttcaccatag cgccgaagga cgatatgccg ctgccgctgt
     3421 tccaagtgtg gttgaacgcg ctggcccgtc gtatgacgga ggacaagtga tggtcgagat
     3481 ttccataacc aatctggtgc tggcgatcat cgcgatggat cgcgacatca aggcgcggga
     3541 aaaggcgctt cgtgatggtt tgatctccga ggcgaatgag gatgaggaga ctcaatacgt
     3601 ccttgacctc tggaaagcgc gcagcgaact tatcggtgcg catgacagag cccgcgagcg
     3661 cgacccgaag ctgtcgccga tcaaactcga ttagatgctc tcactcctcc tcgtggaaca
     3721 gatgagcgta ggcgactgcg acccgcgccg catcggcgcg gtcgcgcggc cctgcttgat
     3781 gccgcgacaa gcgcccaagg acgatatgcc cctgccgctg ttccaagcgt ggttgaacgc
     3841 attgggccgt cacctcgtgg a
//
LOCUS       DASYNS010000475         8851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4797859, whole genome shotgun sequence.
ACCESSION   DASYNS010000475 DASYNS010000000
VERSION     DASYNS010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4797859"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VGP50_14760"
     CDS             complement(<1..533)
                     /locus_tag="VGP50_14760"
                     /inference="COORDINATES: protein motif:HMM:NF044451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8028693.1"
                     /translation="MYYVIEISGVTRYVADMEPGKAFRGRREEYTEATRQALLAAARN
                     AFATDGYQAAGIEEISRAARVTRGAFYHHFEDKKALFDAVVTALQAEASEKIEKRART
                     KKDIWDRLSVGIDAYLDVCLEPAYARIVVQEAPIVLGNKRFREIEESFPMALLEATLR
                     ALKERGQLRFGDIGLLCR"
     gene            complement(699..2633)
                     /locus_tag="VGP50_14765"
     CDS             complement(699..2633)
                     /locus_tag="VGP50_14765"
                     /inference="COORDINATES: protein motif:HMM:NF025314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HEV8028694.1"
                     /translation="MHVPPGPVAARRRPRSLPAALLLIAAASARAQDANVIPAQFIAK
                     MYTEALGRAPDQPGWSRSVAFFAAQGCDAATLRQLGAEFYTSPEFARDYADNAARVLA
                     LYRGALNRDADQAGLDGDVALLDAGVPWPQVVAALFDSAEFAGNVPSMCSATAPDYHF
                     GDQVPPIPVPGRDGFAGTAAELQSILGRAAAGATVYLAQKAVITIDATLTVPSGVTLA
                     TVGAPAATSYALMARLVRGTSFDGPVVAVSPGAQLAHVWVDGQRQVLGYHKVAGGTGD
                     NANIVTLGGAGIAILGNKLSDPQGGSNFFSTGAPCSDEIVRGNLVTAYSAVHDISGFS
                     DGLTMACEGLDLEDNDIVDVTDVAIVLFATPGVTQHSLIAHNRIVSAGLSTNAAISAD
                     PSTGNPGGSSPDYTGTLFADNLFWTGPYTSFDFGIEAGGRPFFSLAADNSNGSGATYT
                     NNTTGSLSARVRAGIAVAGMLDVTVANDGAHPLDLIPVALAPGAPAALCPGGAVIAET
                     SAGNASGTFPPPTVDGYFDGCVNPRFVAIPSSAASVTVSALTFDAASGTFDGTITIEN
                     IGEASLTGPFGIVFTALSGGAALVNATLTVGVTPFLAFPEVLSLAPGETATVATRFAN
                     SSTAPIAFKPLLFSGVPAQI"
     gene            2840..3112
                     /locus_tag="VGP50_14770"
     CDS             2840..3112
                     /locus_tag="VGP50_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028695.1"
                     /translation="MRRAVEASIVVTLLCLLVVAPAAAADTPPPTSDPGHEIGEGFTK
                     IGHGIRDGAVNTWGAVKSIFNGSNNTSDKKESSASSKASGTSPDSK"
     gene            complement(3128..4105)
                     /locus_tag="VGP50_14775"
     CDS             complement(3128..4105)
                     /locus_tag="VGP50_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV8028696.1"
                     /translation="MDPLSDVLSLLKPRSYIFGGFDAGGEWSIAFGQHEGIKCYAVAS
                     GECWLSVEGVPDAVRLKTGDCFLLPRGRPFHVASDLAVTPVEARTLFPAPLNGGIAVL
                     NGGGDFSTVGGHFALAGNHAGILLGMLPPIVHLRKESDKAALRWSMERMRQELREPQP
                     GGFLIAQHLAYMMLVQALRLHLADGLQGGVGWLFALADKQMSAAIKAMHDDPAHRWTL
                     EELAKRAGMSRSTFALKFKETVGWSPMDYLTRWRMLLAGDRLVNSSAAISVIARSFGY
                     DSESAFRTAFKRVMGCAPRHYGRGRTAAAASQGDGDAARASDRLEPVAG"
     gene            4236..5129
                     /locus_tag="VGP50_14780"
     CDS             4236..5129
                     /locus_tag="VGP50_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8028697.1"
                     /translation="MRVFVTGATGFIGSAIVQELLSAGHQVLGLARSEAAAKSLAAAG
                     AKIHRGDLADLDSLRRGAAAADAVIHTAFIHDFAKFAANCETDRRAIEALGAAVAGSD
                     RPLIVTSGTAFLAPGRRATEDMAPSSDAHAIPRVSEQTALSMAPEGVRASVVRLPPSV
                     HGDGDHGFVPRLIGFAREKGVSAYIRDGLNHWPAVHRLDAARLYRLVLEKGSAGARYH
                     GVADEAVPFRDIAAVIGRRLDLPVVSKTPEEAAGHFGWFAPFAAIDCPASSQRTREAL
                     GWQPKQPGLIPDLDHTRYFET"
     gene            5420..7135
                     /locus_tag="VGP50_14785"
     CDS             5420..7135
                     /locus_tag="VGP50_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV8028698.1"
                     /translation="MSKRVADVLIETLEAAGVKTCYGIVGDTLNRIAHAIDQSEIDWV
                     HMRHEEAGAFAAQAEALLTGRLTACAGSCGPGSLHFINGLYEANRNRAPVILIASQIV
                     RHDLGFEAIQEVALDEVFKGCSVFCEMILTPEQARRKTVAACQAALTRRGVAVLVVPA
                     DIANAAAPDEPPYAVHARHPLIRPSDADLDQIAAILNKSEAITIYAGAGCAGAHDEVV
                     ATAARLKAAMAHTSRGKDFVEYDNPYNVGMTGIIGGAAGYHAILDCDVLLLLGADFAW
                     TQFYPDKAKILQIDVDPTHIGRRHPVTIGAVGDIKTTLEALLPRLEQHEDSRFAANYI
                     ERHRKDVAKAKAESVSGPDAAISGTYLTQIINRHAAEDALFAADDGTVGVWMLRHIDT
                     GGKRRTFASLLHGTMASGMSSALGLQKCQPGRQVICLAGDGGFAMLLGELLTTVQEDL
                     PIKIVVYDNGKLGFVDIEQRAAGLVPVYTDLKNPNFGEVAKAIGLWGRFVATAGELED
                     SVKSWLAQPGPALLHVKVKPMQLVIPPSPFVAPEAVVGMAVYSARAMLQGKGHDVWEM
                     AVENI"
     gene            7209..>8851
                     /locus_tag="VGP50_14790"
     CDS             7209..>8851
                     /locus_tag="VGP50_14790"
                     /inference="COORDINATES: protein motif:HMM:NF016513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEV8028699.1"
                     /translation="MTLAARERKDVSRREQAVARGGDRDAGHASPPALPPARDARDLP
                     KPAPQASAKPEQRPVYRGLIAADAEAALFSLKSFAAAMGAYYIALSVGLAQPVWAVTT
                     VYILSQPLAGAVLSKAFFRVLGTVLGAAAAVLFLPAFVNEPAVLSVVLALWLGLCIYL
                     SQLDRTPRSYVFLLAGYTASIIGFPSVLAPGSIFDVAVLRVQEIGIGIAAATLVHGAV
                     FPRSVTDRLRQQITAILGSAEQGSRLALAGTRDPKLDRERRQLASAITGIGQLAFHVA
                     FDTARLLPRAALIRALQDQLCWLPPLTAAIEDRIAECTAREGGLPSDIAALVAQVESW
                     LVHDIAGPARDATARLLVAEAERLEAVFDAEATWRWREILLVSLLARLSELVLVHRLL
                     RELYDHLAGASIRSLSPEAARLIGSATGRSFHRDRGLALRTALGAVVAVSGVCAFWIA
                     TAWPSGAVAALIVGVGCALFGTLPQASIAIRLFFVGALVGIMAAAFFGFAIFPRVTDF
                     VMLAAFMALPLLIFGSMMVRPLLAAIGLGGVLGFVNTVGL"
BASE COUNT         1436 a         2966 c         2993 g         1456 t
ORIGIN      
        1 cggcacagca aaccgatgtc gccgaaccga agctgcccgc gctccttcag cgcgcgcaac
       61 gtcgcctcaa ggagcgccat gggaaaggac tcctcgattt cccggaaacg cttgttaccg
      121 aggacgatcg gcgcttcctg gacgacgatg cgcgcgtagg ccggctcgag gcagacatcg
      181 agataggcgt cgatccccac gctcaggcga tcccagatat ccttcttggt cctggcgcgc
      241 ttctcgatct tctcgctcgc ctcggcctgg agcgcggtga ccaccgcatc gaagagcgcc
      301 ttcttgtctt cgaaatggtg atagaacgcg ccgcgggtca cccgcgccgc ccgcgagatc
      361 tcttcgatcc cggcggcctg atagccgtcc gttgcgaagg cgttccgagc cgcggcgagc
      421 aacgcttgcc gtgtcgcttc cgtgtattcc tcgcggcgtc cccgaaaagc cttgccgggt
      481 tccatgtcgg ccacataccg cgtgacgccg gaaatttcta tgacataata catactatca
      541 atatgtgaaa cgtactgctg gccaacgcgc tggcgaccgg gctggctctc tcgccgtgat
      601 cgaatgcctc gcgccgctgt cgggcgcgca tctcccggga gctggcccgc atcggcgagc
      661 ccgtctcccg accggaacca ggtcgcgcca tccggccact aaatctgcgc ggggacgcct
      721 gagaaaagga gcggcttgaa ggcgatcggc gcggtcgagg aatttgcgaa ccgcgtcgcc
      781 accgtcgccg tctcgccggg ggcgagggac aggacctcgg gaaaggccag gaacggcgtc
      841 acccccacgg tcagggtcgc gttgacgaga gccgcgccgc cgctcagggc ggtgaagacg
      901 atgccgaagg ggccggtgag gctggcctcg ccgatattct cgatggtgat ggtgccgtcg
      961 aaggtgccgc tcgcggcgtc gaaggtgagc gccgacaccg tcaccgaagc agcgctggat
     1021 gggatcgcga cgaagcgcgg gttgacgcag ccgtcgaagt agccgtcgac ggtcggcggc
     1081 gggaaggtgc cgctggcatt gccggccgag gtctcggcga tgacggcgcc gcccgggcac
     1141 agcgccgccg gcgcgccggg cgccaaagca acggggatca ggtcgagggg gtgagcgccg
     1201 tcattggcga cggtcacgtc gagcatgcca gccaccgcga tcccggcgcg cacgcgcgcg
     1261 ctcagcgacc cggtcgtgtt gttggtgtac gtcgccccgg acccgttgct gttgtcggcg
     1321 gcgaggctga agaagggccg tccgcccgcc tcgatgccga agtcgaagct cgtatagggc
     1381 ccggtccaga agagattgtc cgcgaacagc gtgccggtgt agtcgggcga cgaaccgccg
     1441 gggttgccgg tgctggggtc ggcggagatc gcggcattgg tcgagaggcc ggccgagacg
     1501 atgcggttat gcgcgatcaa cgaatgctgc gtcacgcccg gcgtggcgaa gaggacgatc
     1561 gcgacgtcgg tgacgtcgac gatgtcgtta tcctcgagat cgaggccctc gcacgccatg
     1621 gtcaagccgt ccgagaagcc ggagatgtcg tggaccgcgc tataggccgt caccagattg
     1681 ccgcgcacga tctcgtccga gcagggcgcg ccggttgaaa agaaattgct gccgccttgc
     1741 gggtccgaca gcttgttgcc gagaatggcg atgccggcgc cgccgagcgt gacgatgttg
     1801 gcgttgtcgc cggtgccgcc cgcgaccttg tgatagccca gcacctggcg ctggccgtcg
     1861 acccagacat gggcgagctg cgcgcccggc gaaaccgcga cgaccggtcc gtcgaagctc
     1921 gtgccgcgga cgagccgcgc catcagcgcg taggacgtgg ccgccggcgc tccgacggtc
     1981 gcgagcgtaa cgccgcttgg aacagtgagc gtggcgtcga tggtgatgac ggccttctgc
     2041 gccaggtaga cggtcgcgcc ggcggcggcg cgaccgagga tggattgcag ctcggccgcg
     2101 gtgccggcga agccgtcgcg gccgggcacc ggaatcggcg gcacctggtc gccgaaatga
     2161 tagtcgggcg cggtcgcgct gcacatgctg ggcacgttgc cggcgaattc ggcgctgtcg
     2221 aaaagcgcag cgacgacctg cggccagggc acgcccgcgt cgaggagagc gacgtcgccg
     2281 tcgaggcccg cctgatcggc gtcgcggttg agggcgccgc gatagagcgc caggaccctc
     2341 gcggcgttgt cggcatagtc gcgggcgaat tcgggcgagg tatagaattc cgcgcccagt
     2401 tggcggagcg tcgcggcgtc gcaaccctgc gccgcgaaga aggcgacgga gcggctccag
     2461 ccgggctggt cgggcgcgcg tcccagggcc tcggtgtaca tcttggcgat gaactgtgcc
     2521 ggaatgacgt tcgcgtcctg ggcgcgggcg gatgcggcgg cgatgagaag gagcgccgcc
     2581 ggcagcgacc ggggacggcg acgggcagcg acgggaccgg gcggaacgtg catcgggctc
     2641 tccccgttga tcggaacgag gggtgatacg cgccgcggcg gggtctcccc cgcggaacca
     2701 gaaccagttc gcgccagccg gccgccgcca tggcaattgg cgaaaatttt gccgaaattg
     2761 caccgggaat tgtcacttcg ttcacggcac gggaatagac agagtacggg tccgtctggt
     2821 tatcccctgg agaacagcca tgcgccgcgc tgtcgaagcc tcgatcgtcg tcacgctgct
     2881 ctgcttgctc gtggtcgcgc cggccgcggc tgcggacacg ccgccgccga cctccgatcc
     2941 cggacacgag atcggcgaag gcttcaccaa gatcggccac ggcatcaggg atggcgcggt
     3001 caatacctgg ggcgccgtca aatcgatctt caacggcagc aacaatacct cggacaagaa
     3061 ggagtcgtcc gcgtcgtcca aggcatctgg caccagtccg gacagcaagt agcggcgagg
     3121 cgctccgtca gcccgcgacc ggttcgagcc ggtcgctggc gcgagcggcg tcgccgtcgc
     3181 cctgggaagc ggcggccgct gtccggccgc ggccatagtg ccgcggcgcg cagcccatca
     3241 ctcttttgaa ggcagttctg aaggcgcttt cggaatcgta gccgaacgac cgggcaatga
     3301 cggaaatggc agcgctggaa ttcaccagcc tgtcgccggc cagcagcatc cgccagcgcg
     3361 tcaggtagtc catgggcgac cacccgaccg tctccttgaa tttgagcgcg aaggtcgatc
     3421 gcgacatgcc cgcccgcttc gccagctcct ccaatgtcca gcgatgcgcg gggtcgtcat
     3481 gcatcgcctt gatcgccgcg ctcatctgtt tgtcggcgag cgcgaagagc cagccgacgc
     3541 cgccttgcaa tccatccgcc agatgaagcc gcagcgcctg gaccagcatc atgtaggcga
     3601 ggtgctgcgc gatcagaaag ccgcccggct gcggctcgcg cagctcctgc ctcatccgct
     3661 ccatcgacca gcgcaacgcc gccttgtccg attccttccg gagatggacg atcggcggca
     3721 gcattccgag gaggatgccg gcatgattgc cggcgagcgc gaaatgcccg ccgaccgtcg
     3781 agaaatcccc gccgccattg agcacggcga tgccgccatt cagcggcgcg ggaaagagcg
     3841 tgcgcgcctc gaccggcgtc acagcgagat cgctcgcgac gtggaaaggc cggccccgcg
     3901 gcagcaggaa gcagtccccc gtcttcagcc gcaccgcgtc gggcacgccc tcgacggaca
     3961 gccagcactc gccggaagcc acggcgtagc atttgatgcc ttcatgctgg ccgaacgcaa
     4021 tcgaccattc gccgccggca tcgaagccgc cgaagatata gctgcgcggc ttcagcagcg
     4081 acaacacatc ggagagcggg tccatcggcc atcccggacg attgcgaaga taatacggac
     4141 gctagcgcat agatcgtatc gcctcaaatg cctatctcga tgatcccggc tgcgactgcg
     4201 gccatggtca tgagtgaagc agaggagaag tcgtcatgcg tgtctttgtc accggcgcca
     4261 cgggttttat cggctctgcc atcgttcagg agctgctgag cgcgggccat caggtgctcg
     4321 gtcttgctcg ctccgaggcg gccgccaaat cgcttgccgc cgccggcgcc aagatccatc
     4381 ggggtgatct cgcggatctc gacagcttgc ggaggggggc cgccgccgcg gatgccgtga
     4441 ttcacaccgc cttcatccac gacttcgcga aattcgcggc gaattgcgag accgatcggc
     4501 gcgccatcga ggcgctcggc gccgcggtcg ccggctccga ccgccccctg atcgtcacct
     4561 ccgggaccgc gttcctcgcg ccggggcgcc gcgcgaccga agacatggcg ccctcgtccg
     4621 acgcccatgc gattccccgc gtttcggaac agacggccct gtcgatggcg cccgagggcg
     4681 tgcgcgcgtc ggtggtgcgc cttccgccgt cggtccatgg cgacggcgac cacggcttcg
     4741 tcccgcgcct catcggtttt gcccgtgaga aaggcgtctc ggcctatatc cgcgacgggc
     4801 tcaaccactg gcccgcagtg catcggctcg atgcggcgcg tctctatagg ctcgtgctcg
     4861 agaagggctc cgccggcgct cggtatcatg gcgtcgccga tgaggctgtg ccgttccgcg
     4921 acatcgccgc cgtgatcggc cgccgcctcg atctgccggt cgtcagcaaa acccccgagg
     4981 aagcagccgg ccacttcggc tggttcgcgc ctttcgcagc gatcgattgc ccggcttcga
     5041 gtcagcggac acgggaggcg ctggggtggc agccgaagca gcctggcctc attcccgatc
     5101 tcgaccatac ccgctatttc gaaacctgac ggaccacaga agcggcgcgg gtcaccctaa
     5161 acgaaggtgt aggtgaggcg atgctgtgcg acatcgggag gcttcccgac gtgcaatagg
     5221 aatctgccgg gattagccga ctattgtcgc gatgcggtgc aagggcggga ccggcgcgac
     5281 gtccggtgat gcagtcggtg aaccgcggag cctctcgatc atgtcggcaa taccgagcac
     5341 gtcccgagct tcacacgcgc cttttgagta gaggctacga attccaggct ggacggccag
     5401 gcaacgggga ggttcgacca tgagcaagag agtcgccgac gtgctgatcg aaacgctgga
     5461 agccgccggc gtgaagacat gctatgggat cgtcggcgat acgctcaatc gcatcgccca
     5521 tgccatcgac cagagcgaga tcgactgggt ccacatgcgt cacgaagagg ccggcgcctt
     5581 cgcggcgcag gccgaggcgc tgctcaccgg gcgtctcacg gcttgcgccg gaagctgcgg
     5641 tcccggcagc ctccacttca tcaacggcct ctacgaagcc aatcgcaatc gcgcgccggt
     5701 gatcctgatc gccagccaga tcgtgcggca cgacctcggg ttcgaggcga tccaggaggt
     5761 ggccctcgac gaggtgttca agggttgcag cgtcttttgc gaaatgatcc tgacgccgga
     5821 gcaggcgcgc cgcaagaccg tggccgcctg ccaggcggcg ctcacgcggc gcggcgtcgc
     5881 cgtactcgtc gtgccggcgg acatcgccaa tgccgccgcg ccggacgagc ccccttatgc
     5941 cgttcatgcg cggcatccgc tcattcgccc gagcgacgcg gacctcgatc agatcgccgc
     6001 cattctcaac aagagcgagg cgatcaccat ctatgccggc gccggatgcg ccggtgccca
     6061 tgacgaggtg gtcgccacgg cggcgcggct gaaagcggcg atggcccaca cctcgcgcgg
     6121 caaggacttc gtcgaatacg acaatcccta caatgtcggc atgaccggca tcatcggcgg
     6181 cgccgccggc tatcacgcca ttctcgactg cgatgtgctg cttttgctcg gcgcggattt
     6241 cgcctggacg caattctatc cggacaaggc gaagatcctt cagatcgacg tcgatcccac
     6301 gcatatcggg cggcgacatc cggtgacgat cggcgcggtc ggcgatatca agacgacgct
     6361 ggaggctctg ctgccgcgcc tcgagcagca cgaggatagc cgcttcgccg caaactatat
     6421 cgagcgtcac cggaaggatg tggcaaaggc caaggccgag agcgtgtccg gcccggacgc
     6481 ggccatctcc ggtacctacc tgacgcagat catcaaccgg catgcggccg aggacgcttt
     6541 gttcgcggcg gatgacggga cggtcggggt gtggatgctt cgccatatcg acaccggcgg
     6601 caagcgccga acttttgcca gcctgctcca cggcacgatg gcgagcggga tgtcgtcggc
     6661 gctcgggctc cagaagtgcc agcccggccg gcaagtgatc tgtctcgccg gcgatggcgg
     6721 cttcgccatg ctgctcggcg agctgctgac gacagtgcag gaggatctgc cgatcaagat
     6781 cgtcgtctat gacaacggca agctcggttt cgtcgatatc gagcagaggg cggcgggtct
     6841 ggtgccggtt tacacggact tgaagaaccc caatttcggc gaggttgcga aggcgatcgg
     6901 cctttggggg cgcttcgtcg cgacggccgg cgagctcgag gactccgtca agtcatggct
     6961 ggcgcaaccg ggtccggcgt tgctgcatgt gaaggtgaag ccgatgcagc tggtgatacc
     7021 gccctcgccc ttcgtcgcac ccgaagccgt ggtggggatg gcggtctaca gcgcccgcgc
     7081 gatgttgcaa ggcaaaggcc acgatgtctg ggaaatggcg gtggagaaca tctgaccacc
     7141 gcacgagcgg aacggacaga tttgtgcatg cgatggggtt gacgtgagtt tgaccaagtg
     7201 ccgcgccgat gacgctagcc gcacgtgaac gaaaggatgt ctcccgccgc gagcaggctg
     7261 tcgctcgcgg cggagatcgc gatgccggcc atgcgagccc gccggcgctg cctccggcgc
     7321 gggacgcgcg agacctgccc aagccggcgc cccaggccag cgcgaagccg gagcaacggc
     7381 cggtctaccg cggcctgatc gccgccgacg cggaagcggc gctcttttcg ctgaagagct
     7441 tcgcggccgc gatgggcgcc tattacatcg ccctgagcgt gggacttgcc cagccggtct
     7501 gggccgtcac gaccgtctac atcctgtccc agcccctggc gggcgccgtg ctgtcgaagg
     7561 cgttcttccg cgtgctcggc acggttctcg gtgcggccgc agccgtcctc ttccttccgg
     7621 ctttcgtgaa tgaaccggcg gtcctgagcg tcgttctggc gctgtggctc gggctctgca
     7681 tctacctctc gcagctcgac cgcacgccgc gttcctacgt cttcctcctc gccggctaca
     7741 cggcgagcat catcggcttt cccagcgtcc tcgcgccggg cagcatcttc gacgtcgcgg
     7801 tgctgcgcgt ccaggagatc ggcatcggca tcgccgccgc cacgctcgtt catggcgcgg
     7861 tctttccgcg cagcgttacg gaccgtctcc gccagcagat caccgccatc ctcggcagcg
     7921 ccgagcaggg gagccgtctt gccctcgccg gaacgcgcga tcccaagctc gaccgcgaac
     7981 gacgacagct cgccagcgcg atcaccggta tcggacaact tgcctttcat gtggcgttcg
     8041 atacggcacg gctgctgccg cgggccgccc tgatccgcgc gctgcaggac cagctctgct
     8101 ggcttccgcc gctgaccgcc gccatcgaag accgcatcgc cgaatgcacc gcacgggagg
     8161 gcgggctccc gtcggacatc gccgccctgg ttgcccaggt cgaaagctgg ctcgtccatg
     8221 acatcgccgg tcccgcgcgc gacgcgacgg cccggcttct cgtcgccgag gcggagcggc
     8281 tcgaagcagt gttcgatgca gaggccacct ggcgctggcg ggagatactc ctcgtcagcc
     8341 tgcttgcccg cttgtcggag ctcgtcctgg tccatcgcct gcttcgcgag ctctatgatc
     8401 acctcgccgg cgccagcatc cgcagccttt cgcccgaggc ggcccggctt atcgggtcgg
     8461 cgaccggccg gtcgttccat cgcgaccgcg gcctcgcgct ccgcacggcg ctcggcgccg
     8521 tcgtcgcggt ctccggcgtc tgcgcgttct ggatcgcgac agcttggccg agcggggctg
     8581 tggccgcatt gatcgttggt gtcggctgcg cgctgttcgg caccctcccg caggccagca
     8641 tcgcgatccg gcttttcttc gtcggcgccc tcgtcggcat catggcggcg gcgttcttcg
     8701 gcttcgcgat atttccgcgc gtcaccgact tcgtgatgct ggccgcgttc atggcgctgc
     8761 cgctcctgat cttcggcagc atgatggtgc gcccgttgct cgcggcgatc ggcctcggcg
     8821 gcgtcctcgg attcgtcaac accgtcggcc t
//
LOCUS       DASYNS010000476         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2772303, whole genome shotgun sequence.
ACCESSION   DASYNS010000476 DASYNS010000000
VERSION     DASYNS010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2772303"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..546)
                     /locus_tag="VGP50_14795"
     CDS             complement(94..546)
                     /locus_tag="VGP50_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV8028700.1"
                     /translation="MAGRYLEDFHAGEVIELGSRTLSAEEIIAFARQFDPQYFHIDPE
                     GAKAGPFGGLIASGWHTAAIYMRLMVDQFISGMAESMGSPGVDGIRWLKPVRPGDTLK
                     GRITIVDVIPSKSHANRGTLKTLGEVVNQHGEVVMQINAAGFFARRPT"
     gene            639..725
                     /locus_tag="VGP50_14800"
     tRNA            639..725
                     /locus_tag="VGP50_14800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:673..675,aa:Leu,seq:caa)
     gene            complement(857..1252)
                     /locus_tag="VGP50_14805"
     CDS             complement(857..1252)
                     /locus_tag="VGP50_14805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HEV8028701.1"
                     /translation="MRVVVDTNVFISAVLKDQSPPALAVHLVQQRGSLLKSLATEEQL
                     RPRLAPLIAPAARAWLQSLLEAAELVTIAERIQACRDPTDDKFLELAINGRADIIVSG
                     DADLLALNPFREIPIVAPATFVRAAALSG"
     gene            complement(1607..1900)
                     /locus_tag="VGP50_14810"
     CDS             complement(1607..1900)
                     /locus_tag="VGP50_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028702.1"
                     /translation="MSGWSSWQKFYYAWGIAAGALALTILVVLIVPIAIPYAAAILVP
                     VLAVAAVYGVFFVRCPKCGVRLTETVLSHGLPQESCPICLYDLKTQETKPAGR"
     gene            1964..>2688
                     /locus_tag="VGP50_14815"
     CDS             1964..>2688
                     /locus_tag="VGP50_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEV8028703.1"
                     /translation="MRRIGALAGRVEAGILGAARGQRMRDALAENRGRIIVICGLLVA
                     ANVAAWVWALLAFRHHPALFGTALLAYSFGARHALDADHIAAIDNVTRKLMQDGKRPL
                     GVGLYFSLGHSTVVVLASMAIAAAAGTELVGIKAVGSVVGTVVSTLFLFAIAAANIVV
                     LVAVYSSFARIRRGGGYVEEDLDSILAQRGLLGRLFRPLVRLVGASWQMYPLGFLFGL
                     GFDTATEIGVLGIAAAEASHGLSL"
BASE COUNT          452 a          883 c          880 g          473 t
ORIGIN      
        1 gcgcgatgat ccgatcgagc aagggcggct ccctggcaat cctgcggcct tacgagccgc
       61 aatctccggt gcgcggcaat gggggcgcgc gcatcacgtc ggccggcgcg cgaagaagcc
      121 agcggcgttg atctgcatga cgacctcgcc atgctgattg acgacctcgc ccagcgtctt
      181 gagggtgccg cggttggcgt ggctcttcga cggaatgacg tcgacgatcg tgatacgtcc
      241 tttgagcgtg tcgcctggcc gcaccggctt cagccagcga atgccgtcga cgccgggcga
      301 gcccatgctc tcggccatgc cgctgatgaa ctggtcgacc atcaggcgca tgtagatggc
      361 ggcggtatgc cagccgctgg cgatgaggcc gccgaacggg cctgccttcg cgccctcggg
      421 atcgatgtgg aaatattgcg gatcgaactg ccgggcgaag gcgatgatct cctcggcgct
      481 caacgtccgg ctgccgagct cgatgacctc gccagcatgg aagtcctcaa gatagcgtcc
      541 cgccatggcc gcgggtctag ccgggatcag gcgcaggggc aagcgcctcg gacggcgctc
      601 ttgcatcgcg tccaggcgaa agctaccact ccgcccgtgc ccctctggcg gaactggtag
      661 acgcacggga ctcaaaatcc cgcgatcgca agatcatgtc ggttcgattc cgacgggggg
      721 caccatcacg cttcgatgcc gcatcagcgg ggagccggct tcgtcggcgg cctcgatgtt
      781 tcggcgagcg gcgcgatctc aaaaagaggc gattgcttcg gctcatgtaa gccgtccgac
      841 taccccgatc ccgccgttat cctgagagcg cagcagcgcg cacaaaagtg gcgggcgcca
      901 caatgggtat ctcacgaaac ggattgagcg cgaggagatc ggcatctcca gaaacaataa
      961 tatcggctcg cccgttaatc gccaactcga ggaacttgtc gtctgtcgga tcacggcagg
     1021 cttggatgcg ttcggcgatc gtcaccagtt cggccgcctc gagcaagctc tgcaaccatg
     1081 cgcgtgcggc gggtgcgatc agaggggcga ggcgtggccg cagctgctct tctgttgcta
     1141 gggatttgag aagggaaccg cgttgttgca ccaagtgcac ggccaaggcc ggcggggact
     1201 gatcctttag taccgcactg atgaagacat tggtatcaac gacaaccctc atccgagctt
     1261 tggctcggca tcaaattgcg cttgcttaat cagccgatcg agatccccgt cggtcagtcc
     1321 agccgctacg acgcgggcgc gggattcccg gcgtcccggg acaatctcgg gtgtgggctc
     1381 aatcgtgatc gtcacacgct catccgagcc gatcccccgc cgcgcgagct cggccgcgac
     1441 ttcgccggcc gtcgtttcga tgatctaatc ccgcttcatg gaggctccat gttcacggtt
     1501 tgtttatacc acgcggcagg gaccaaaatc aagattggca agatcgtgcc ggttcgactc
     1561 cgacggggca gcacgccccc gcgcgaggcg cagcggcggt gcgccgtcaa cggccggcgg
     1621 gcttcgtctc ctgcgtcttc aggtcgtaga ggcagatggg gcagctctct tgcggcagtc
     1681 catgggacag caccgtctcc gtcagccgca cgccgcattt gggacagcgc acgaagaaga
     1741 cgccgtagac ggcggccacc gcgagcacgg ggaccaggat agcggcggcg taagggatcg
     1801 cgatcggcac gatgaggacc acgaggatcg tgagcgcgag cgccccggca gcaatgcccc
     1861 aggcgtagta gaacttctgc cagctactcc agccgctcat ctccccaccc cgaaggccaa
     1921 gcttcgatgc gcgcgaggat agcgtggcca tggcgtcgct gccatgcgcc ggatcggcgc
     1981 ccttgccggc cgcgtcgaag ccggcatact gggcgcggca agggggcagc gaatgcgcga
     2041 cgcgctcgcg gaaaatcgcg gcaggatcat cgtcatctgc ggcctgctcg tcgcggccaa
     2101 tgtcgcggcc tgggtctggg cgctcctcgc cttccgccac catccggcac tcttcggcac
     2161 ggcgctcctc gcctacagct tcggcgcgcg ccacgcgctc gacgccgacc acatcgccgc
     2221 catcgacaac gtcacgcgca agctcatgca ggacggcaag cgcccgctcg gcgtcggcct
     2281 ctatttctcg ctggggcatt cgaccgtggt cgtgctcgca tcgatggcga tcgccgcggc
     2341 cgcaggaacg gagctggtcg gcatcaaagc cgtgggcagc gtcgtcggca cggtcgtatc
     2401 gacgttgttt ctcttcgcca ttgccgcggc caatatcgtc gtgctcgtcg ccgtctatag
     2461 cagcttcgcg cggatacgga ggggcggcgg ctatgtcgag gaggatctcg acagcatcct
     2521 ggcgcagcgc gggctcctgg ggcgcctctt ccggccgctc gtccgcctcg tcggcgcgag
     2581 ctggcagatg tatccgctcg gctttctctt cggcctcggc ttcgatacgg cgaccgagat
     2641 cggcgtgctc ggcatcgccg ccgccgaggc gtcgcatggc ttgtcgct
//
LOCUS       DASYNS010000477        11275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5422821, whole genome shotgun sequence.
ACCESSION   DASYNS010000477 DASYNS010000000
VERSION     DASYNS010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11275
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5422821"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VGP50_14820"
     CDS             complement(<1..550)
                     /locus_tag="VGP50_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEV8028704.1"
                     /translation="MRLVIAMMKHETNTFSPVRTDWQRFRDWGAHLGAEVPRAYEGTR
                     MPIGAYLKLAREIGAETVTPIAAEAMPSGPVEATAYELMSTAILDAVRKGCDAALLDL
                     HGAMVAQTTPDGEGTLLERIRGIAPDLPIAVTCDLHANLTEKMVRNATVLVGYKTYPH
                     VDMYEMGEQAGRILLRALRGEIR"
     gene            complement(554..976)
                     /gene="hisI"
                     /locus_tag="VGP50_14825"
     CDS             complement(554..976)
                     /gene="hisI"
                     /locus_tag="VGP50_14825"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEV8028705.1"
                     /translation="MTEAGGTGDRAAAVIAALRFDKDGLLPAVAQQHDTGEVLMLAWM
                     NREAVTRTLDTGHVHYWSRARNALWRKGERSGQEQRLVELRVDCDGDTLLLRVEQTGV
                     ACHTGQRSCFFRAAHDGALVTIAEPLMSPGELYGESGH"
     gene            complement(985..1899)
                     /locus_tag="VGP50_14830"
     CDS             complement(985..1899)
                     /locus_tag="VGP50_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEV8028706.1"
                     /translation="MSARPDPEESAPSKGTTSAVEVAITPRSRDLGGFTVRRVLPAIE
                     RRMVGPFIFFDVMGPAAFNAGDGIDVRPHPHIGLATLTYLFEGEILHRDSLGTVQPIV
                     AGDVNWMTAGRGIVHSERSDPKKRLAGARLSGIQSWIALPKRDEETAPAFAHHPAATL
                     PLIEGDGTRVRLIAGTLFGARAPARIFSDMFYADAALAPAARLAFSPEHEERALFIVE
                     GTVEIAGHSYAAGQLLVLHPGAEVVVTAGETVRLMLLGGAPLDGPRHIWWNFVSSSPE
                     RIEAAKADWKARRFPAVPGETEFTPLPE"
     gene            2125..3471
                     /locus_tag="VGP50_14835"
     CDS             2125..3471
                     /locus_tag="VGP50_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEV8028707.1"
                     /translation="MPQDKDAAQSLLEDLIAKARRAGADAADAIAFEGISLSHAQRLG
                     RIEKLERSEGYDLGLRVLIGKRQAIVSANERGGERFGELVERAIAMARAVPEDPYCGL
                     AAPDEIARNWPELDMVDPVEPSPEALIARAAEAETAAQAVPGVTNSEGAEASWGRSRL
                     TLVASNGFAGSFAATGHSISVSVLAGSGTAMERDDDFTTAIHGGDLRDPKMVGRRAGE
                     RAVHRLGARKVATCQVPVVYDPRVSASLLRHLMGAITGPAIARRTSFLKDKLGQQIFP
                     RGLVVVEDPHRRRGLNSRPFDAEGIANRRRAIVEDGVLTTWLLDLASARQLGLKTTGH
                     ARRGPSSPPSPSPTNLWLEPGPQTAGELIGDVTRGFYVTELMGMGVNGVTGDYSRGAA
                     GFWIEAGELAYPVSEVTIAGNLKDMFQRIVAANDLEFRTGIDAPTIRVDGMTVAGA"
     gene            complement(3688..4614)
                     /locus_tag="VGP50_14840"
     CDS             complement(3688..4614)
                     /locus_tag="VGP50_14840"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV8028708.1"
                     /translation="MGVAPRRARRLLVIYNPTAGRRARWRLARWLACLDRLGATVTLS
                     ETQGPRHAEALASAADPARYDAIAVAGGDGTINEVVNGLAGSSLPLAILPLGTANVLA
                     AEIGLPRRIAELARIAALAPARPVWPGEAQADGAPAGRRFLVMAGLGFDAEVVEHLDL
                     ALKRRTGKFAYVVSIFGRLRDYRPCSYRALLDGVPADGASLVAAKSHFYGGRFVLAPA
                     ARLDEPGLEVVLFKHAGRRAAIGYLLAMGLGLLPRCPSVSIVRAQEIRLMEPTGAPIQ
                     LDGDIQLRLPARLRIAAVPLQLIQPECPATGL"
     gene            4783..6627
                     /gene="msbA"
                     /locus_tag="VGP50_14845"
     CDS             4783..6627
                     /gene="msbA"
                     /locus_tag="VGP50_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007091220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEV8028709.1"
                     /translation="MAPRGITVANKGHHPALEIAEFSHDTLPLVGRLLRDFLRPYLGR
                     VAAAFLCMALVAGATAANAWLMQPMLDRVFVAHDEHLLWVIPLVVVVLALVKGLAGYW
                     QSVAMTTVGQRVVADIQLSLFARLMGADLSYFHANPTGSLISRFTNDANMLRGATTTV
                     LAGIGKEAVTALFLIALMFYQDWVLALASFIVFPLAIRPLVSIGRRMRRVSVDTQSEL
                     GEFTTLLDQTFQGARHVKAYGMEAYETNRAGQLIERLYDLVERAQRTRSIASPLMETL
                     GGIAVAIVILYGGHQVITGLRTPGTFFSFITALLLAYQPVKALAGLNANLQEGLAAAQ
                     RIFTVLDVEPEIREAPNARPLALRGGEIRFRDVRFAYANGAVALDDVTLAVPPGKTVA
                     LVGASGSGKSTILNLIPRFYDVAAGAVAIDGMDVRDATLASLRSAIALVSQEVSLFDD
                     TVRANIAYGRFGASEAEIVAAAKAAAADDFIRALPEGYDTPVGEHGVKLSGGQRQRLS
                     IARAMLKNAPILLLDEATSALDTESERQVQAALRTLMRGRTTLVIAHRLSTVVDADLI
                     HVIDGGCVVESGTHVELLRQNGIYARLYAAQFADQTALSAATLRAVGG"
     gene            6642..7382
                     /locus_tag="VGP50_14850"
     CDS             6642..7382
                     /locus_tag="VGP50_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV8028710.1"
                     /translation="MKRLLRTATAHQILCWFIHLYIRFVYATSRWTVEGGDIPRRLRD
                     AGRPFILAFWHGRLLMIPMAWQRLAPMHMLISGHRDGRIIAGAVRYFDVDSIAGSSNQ
                     GGTGALRAMVRHLRDGDCVGITPDGPNGPAMRATPGIVAAAKISQAPVIPITYATSRR
                     RILRSWDRFHLALPWSRGVYLWGEPITVPDDLGEGGAELWRRRIEDFMIAQTAEADRR
                     VGQEVITPGTLQRDAIRALRRAPQGEAR"
     gene            7379..8677
                     /locus_tag="VGP50_14855"
     CDS             7379..8677
                     /locus_tag="VGP50_14855"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HEV8028711.1"
                     /translation="MSWLRLYRALTTVLGPVVMVYLARRLVSGKEDRVRFAERRGVAG
                     IERPPGPLVWVHAASVGEAVSMLALIDRLLAERPLISVMVTTGTVSSARLLAARLPAN
                     RAWHQYIPVDRIAYVRRFLDHWRPDLALWVESELWPNLILETKARDIPLLLINARMSA
                     RSFQGWLRWPAIINEVLGAFDLCLAQDVVQAERLRQLGARRSLCVGNLKSAAGPLPAS
                     ESELVRFAAQAGGRPLWLAASTHPGEEEAAAAVHRRLKARWPDLLTLLVPRHPARAAA
                     VAAMLKREGLAVARRSLAEPITSATDVYLGDTLGELGLFYRLAGIAFVGGSLVPMGGH
                     NPLEAALLDCAVLHGPDMSNCASIARDLATAGAAVAARDADELAEAVGRLLADPAERA
                     RRAAAGLEVAADNRAVLDAVVERISPWLDRLTPHATSAYA"
     gene            8664..9629
                     /gene="lpxK"
                     /locus_tag="VGP50_14860"
     CDS             8664..9629
                     /gene="lpxK"
                     /locus_tag="VGP50_14860"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEV8028712.1"
                     /translation="MPTPEFWARRGFVSSLLQPLAWAYAAAVAARWQWTRGVTAAVPV
                     ICVGNLVAGGAGKTPVVLSLARRLKAAGRAVHIVSRGYGGRLAGPIAVDPRSHRADDV
                     GDEALLLAEAAPTWVARDRAAGAERAVAAGASVLLLDDGLQNPGLAKDLSLVVIDGQF
                     GIGNGRVLPAGPLREPFDTGVARADAVILIGTDDMRVEPLLAGTSVLRARLVAENADR
                     FCGHAVSAFAGIGRPTKFFATLEAAGARVIARRAFADHHPYREDELRRLQEEAVGSGA
                     TLVTTAKDWVRLSPEWRSRAEVLHVSIRWEDELALERTMAPVLRH"
     gene            9727..10437
                     /locus_tag="VGP50_14865"
     CDS             9727..10437
                     /locus_tag="VGP50_14865"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEV8028713.1"
                     /translation="MGSDIAEVLAVGGFAVIPAAMPSNVVTRLLLLFEPDPNAMGGDA
                     KGRYAMRNVAELIPEVRDVADGFTIRSLIDPILGREAFLVRSLFFDKTARANWKVAWH
                     QDRSIAVQQRTEVAGFGPWSVKAGVAHVAPPVATLEEMLTMRLHLDDCDESNGPLRVI
                     PGSHLKGRLTARQIRDEIAERPQVSCPVAAGGVLLMRPLLLHASSPARPPRHRRVVHL
                     EFARSRLPDGLRWACEAR"
     gene            complement(10480..>11275)
                     /locus_tag="VGP50_14870"
     CDS             complement(10480..>11275)
                     /locus_tag="VGP50_14870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEV8028714.1"
                     /translation="TPCTAGTLSLDGTVEQGALVRGKADPGATVTLDGRAVRVAPDGS
                     FIFGFGRDAPNHASLDVAFPDGGHEHRDLSVTARSYDIQRIEGLPQQTVTPDAAQMER
                     VAREGEIIKRARAADTDLLFFEEPLRWPALGPISGVYGSQRILNGEARAPHAGVDVAA
                     PRGSPVAAAAAGTVTLAERDLFFTGGTVIIDHGYGLATTYQHMDRVDVKLGQHVEAGE
                     PIGIVGATGRVTGPHLHWALNWYEVRLDPALAVGPMPQTTAPQAGH"
BASE COUNT         1694 a         3834 c         3827 g         1920 t
ORIGIN      
        1 ggcggatctc ccctctgagg gcgcgcagca ggatgcggcc ggcctgttcg cccatctcgt
       61 acatgtccac atggggatag gtcttgtagc cgacgagcac ggtcgcgttg cgcaccattt
      121 tctcggtgag attggcgtgg aggtcgcaag taacggcgat cggcaggtcg ggggcgatgc
      181 ctcggatgcg ctccaacaga gtgccttcgc cgtcgggcgt cgtctgcgcc accatcgcgc
      241 catgcagatc gagaagagcc gcatcacaac ccttgcgcac ggcgtcgaga atggcggtcg
      301 acatgagctc gtaggccgtc gcctcgacgg gtccgctggg catcgcctcc gcggcgatcg
      361 gcgtcaccgt ttcggcgccg atctcacgcg cgagcttcag ataagcgccg atcggcatgc
      421 gcgtgccttc gtaggcgcgc ggcacttcgg cccccagatg ggcgccccag tcgcgaaagc
      481 gctgccagtc ggttcgcacg ggtgagaagg tgttggtctc gtgcttcatc atggcgatga
      541 cgagacgcat gactcagtgc ccgctctcgc catagagttc gccagggctc atcagcggct
      601 cggcgatcgt cacgagcgcg ccgtcatgtg ccgcgcggaa gaagcagctg cgctgcccgg
      661 tgtggcaggc tacgcccgtc tgctcgacac gcagcagcag cgtatcgccg tcgcagtcga
      721 cgcgcagttc gaccaagcgc tgctcctgac cggagcgctc gcccttccgc cagagcgcgt
      781 tgcgcgcgcg cgaccagtaa tggacgtggc ccgtgtcgag ggttcgcgtc acggcctcgc
      841 gattcatcca ggcgagcatc agcacctcgc ccgtatcatg ctgctgcgcc acggccggga
      901 gaagtccatc cttatcgaag cgcagcgccg cgatcacggc cgccgcacga tctcctgtcc
      961 ctccggcctc ggtcatcgct cttctcactc gggtagcggc gtgaattccg tctcgcccgg
     1021 aaccgccgga aagcggcggg ctttccaatc ggccttcgcg gcctcgatgc gttcgggcga
     1081 gctcgacacg aagttccacc agatgtgacg cggcccgtcg agcggcgcgc cacccagcag
     1141 catcaaccgg acggtctcgc ctgccgtgac gacgacctcg gcgcccggat ggaggacgag
     1201 caattgtccg gcggcatagg agtgtccggc aatctccacc gtgccctcga cgatgaagag
     1261 cgcacgctcc tcatgctccg gcgagaaggc gagacgcgcc gccggcgcga gggcagcgtc
     1321 ggcgtagaac atgtccgaaa atatccgcgc cggcgcgcgg gcgccgaaga gggtaccggc
     1381 gatgagccgc acgcgcgtgc cgtcgccttc gatgagcggg agcgtggcgg cagggtgatg
     1441 agcgaacgcc ggcgcggtct cctcgtcgcg tttggggagc gcgatccagg actgaatgcc
     1501 ggaaagccgc gctcccgcca ggcgcttctt gggatcgctg cgctcggaat ggacgatgcc
     1561 gcgcccggcc gtcatccagt tcacgtcacc cgcgacgatc ggctgcacgg tgccgaggct
     1621 gtcgcgatgc aggatttcgc cttcgaagag gtaggtgagc gtcgcgagcc cgatatgcgg
     1681 atgcggccgc acatcgatgc cgtcccctgc gttgaacgcc gccggcccca tgacatcgaa
     1741 gaagatgaag ggtccgacca tgcggcgctc tatcgccggc agcacgcggc gcaccgtgaa
     1801 gccgccgagg tcgcggctgc gcggcgtgat cgctacctcg acggcgctcg tcgtcccttt
     1861 cgatggagcc gattcttcgg ggtcgggccg cgcgctcatg gtgtccatcc cgttaacgtc
     1921 caagcgtccc tatacatggg aacgcggccg tacggcaaca tcccggggtc tggctgcgtc
     1981 cccgcggtta agctgtcttc agcgtcacgc gtatggcggc ttcgccaagg cgggttcctg
     2041 acgtcatcgg tgttgcgcgc tggcggaaga ggcgcggttc cctgatataa tgggcttccg
     2101 atcgcgccct ttgcgaggaa gcccatgccg caagacaagg acgccgccca gagcctcttg
     2161 gaggatctca tcgccaaggc ccgccgcgct ggcgccgatg ccgcggacgc catcgccttc
     2221 gagggcatct cgctcagcca tgcgcaacgc ctcggacgca tcgagaagct cgagcgatcc
     2281 gaagggtacg acctcggtct gcgcgtgctc atcggcaagc gccaggcgat cgtttccgcc
     2341 aatgagcggg gcggcgagcg ctttggcgag ctggtcgagc gagccatcgc catggcgcgc
     2401 gccgtgccag aggatcccta ttgcgggctc gctgcgcccg acgagatcgc gcgaaactgg
     2461 cccgagctcg acatggtcga tccggtggag cccagccccg aggcgctgat cgcgcgcgct
     2521 gccgaggcgg agacggcggc ccaggcggtg cctggcgtca ccaactccga gggagccgaa
     2581 gcaagctggg gccgctcgcg cctgaccttg gtcgcctcca acgggtttgc cggcagcttc
     2641 gcggcgaccg gccacagtat cagcgtctcg gtgctcgccg gcagtggcac ggccatggag
     2701 cgcgacgacg acttcacgac ggccatccac ggcggcgacc tgcgcgaccc gaaaatggtc
     2761 ggccgtaggg ctggcgagcg cgcggtgcac cgtttgggcg cgcgcaaggt agcgacgtgc
     2821 caggtgcctg tggtgtacga tccgagggtc tccgcgagtc tgctgcggca tctcatgggg
     2881 gcgatcaccg gccccgccat cgcgcgccgt acgagctttc tcaaggacaa gctcggccag
     2941 cagatctttc cccgcggcct cgtcgtcgtc gaggatccgc atcgccggcg cgggctgaat
     3001 tcgcggcctt tcgacgccga gggcatcgcc aaccggcgcc gcgccatcgt cgaggatggt
     3061 gtgctcacga cctggctgct cgatctcgct tcggcgcgcc aactcggtct caagaccacc
     3121 gggcatgcgc ggcgtgggcc atcgtcgccg ccctcgccat cgcccaccaa cctgtggctc
     3181 gagccgggac cccagaccgc gggtgagctg atcggcgacg tgacgcgcgg cttctacgtg
     3241 accgagctca tgggtatggg cgtcaacggc gtcaccggcg actacagccg cggcgccgcc
     3301 ggcttttgga tcgaggctgg cgaactcgct tatcccgtca gcgaggtgac catcgccggc
     3361 aatctcaagg acatgttcca acgcatcgtc gccgccaacg atctcgagtt ccgtaccggc
     3421 attgacgctc ctaccatccg cgtcgacggg atgacggtgg cgggcgccta ggagcgttgg
     3481 tgccagcccg tggcgcacga gtccggctcg aaagaccgga gcaccgatgc gttggagcag
     3541 atatcgagta agtagaatcg gcacttccta agatcgtcat tcccgcgaaa gcgggaaccc
     3601 agagcaacgc gcacgtcgct tacccctggg gccccgcttt cgcggggatg acgacgaagg
     3661 aacggttcaa catagacggg atgcgctcta gagacccgtc gctggacatt ccggctggat
     3721 gagctggagt ggcaccgcgg cgatgcgtag ccgagccggc aggcggagct ggatgtcgcc
     3781 atcgagctgt atgggcgcgc ccgtcggctc catgaggcgg atctcctggg cgcggacgat
     3841 gctcacgctt gggcagcgcg gcaagagccc gaggcccatg gccagcagat agccgatcgc
     3901 ggcgcggcgc ccggcatgct tgaacagcac gacctcaagc cctggctcgt cgaggcgcgc
     3961 ggccggcgcg aggacgaagc gaccgccgta gaaatgcgat ttggcggcga ccagcgaggc
     4021 gccatcggcc ggcacgccgt cgagcagggc gcgatagctg caaggccgat agtcgcgcaa
     4081 acggccaaag atgctgacga cataggcgaa cttgccggtc cggcgcttga gcgcgagatc
     4141 gagatgctcc acgacctcgg catcgaagcc caagcccgcc atcacgagaa aacggcggcc
     4201 cgctggtgcg ccgtccgcct gcgcttcgcc gggccagacc ggccgcgccg gcgccaacgc
     4261 ggcgatccgt gccagctcgg caatcctgcg agggagcccg atttcagcgg cgagcacatt
     4321 ggcggtgccc agtggcagga tggccaaagg cagcgacgac cccgcgaggc cattcacaac
     4381 ctcgttgatg gtgccatcgc cgcccgcgac ggcgatggcg tcgtagcggg cggggtcggc
     4441 ggcgctggcc agcgcctcgg catgccgcgg cccctgcgtt tcggacagcg tcactgtcgc
     4501 tcccagccgg tccaggcagg cgagccagcg cgcgaggcgc caccgcgcgc gccgtccggc
     4561 cgttgggttg tagatgacga gaaggcggcg ggcccgccgc ggagcgaccc ccatggggcc
     4621 ttccataccg caaggcgcga gtgccgtcat gcgacatctc gcgcggcgcg cttgcctgcg
     4681 ccccttcggt catctacatc agccgtaaac gccctttcgg gtaagcaagg gcgaccttcg
     4741 cgcgactccc cgtcgccttt ggggagaagg caaggcgcgg gcatggcgcc gcgtggtata
     4801 accgtcgcga acaagggaca tcatcccgct ttggagatcg ccgaattcag ccacgatacc
     4861 ctgccgctgg tcgggcggct gctgcgcgac ttcctacgcc cctatctcgg ccgcgtggcg
     4921 gcggcgttcc tctgcatggc gctggtggcc ggggcgaccg cggccaatgc ctggcttatg
     4981 cagccgatgc tcgaccgcgt cttcgtcgcc catgacgagc atctgctctg ggtgatcccg
     5041 ctcgtcgtcg tggtgctggc gctggtcaag ggtctcgccg gctattggca gtccgtcgcc
     5101 atgaccaccg tcggacagcg ggtcgtcgcc gacatccagc tttcgctctt cgcccgattg
     5161 atgggcgccg acctctccta tttccacgcc aatcccacgg gcagcctgat ctcgcgcttc
     5221 accaacgacg ccaacatgct gcgcggggct acgacgaccg tgctcgcggg catcggcaag
     5281 gaagcggtga ccgcgctctt cctcatcgca ctcatgttct atcaggactg ggtgctggcg
     5341 ctcgcctcgt tcatcgtctt tccgctggcc attcggccgc tcgtcagcat cggccggcgc
     5401 atgcggcgcg tctcggtcga cacccagagc gagctcggcg agttcaccac gctcctcgat
     5461 cagaccttcc agggcgcgcg ccacgtcaag gcctatggca tggaggccta tgagacgaac
     5521 cgcgcggggc aactcatcga gcggctctac gacctggtcg agcgcgcgca acgcacccgc
     5581 tccattgcct cgccgctgat ggagaccctc ggcgggatcg ctgtcgccat cgtcatcctc
     5641 tatggcggac atcaggtgat cacgggactg cgcacgcccg gcaccttttt ctccttcatc
     5701 acggcgctgc tcctcgccta tcagccggtg aaggcgctgg cgggtttgaa tgccaatctc
     5761 caggaaggcc tcgccgcggc gcagcgcatc ttcaccgtcc tcgatgtcga gcccgaaatc
     5821 cgcgaggcgc cgaacgcccg tcccctggca ctccggggtg gcgagatccg cttccgggac
     5881 gtgcgtttcg cctatgccaa tggtgcggtt gcgctcgatg acgtgacgct cgccgtgcca
     5941 cccggcaaga ccgtggcgct ggtcggcgcc tcgggcagcg gcaagtccac catcctcaac
     6001 ctcatcccac gcttctacga cgtggcggcc ggcgccgtcg ccatcgacgg catggatgtg
     6061 cgcgatgcca cgcttgcctc attgcgcagc gcgatcgcgc tggtgagcca ggaagtgagc
     6121 cttttcgacg acacggtgcg cgccaacatc gcctatggcc gcttcggtgc cagcgaagcc
     6181 gagatcgttg ccgctgccaa agcagcggca gccgacgact tcattcgagc gctccccgag
     6241 ggttatgaca cgcccgtggg cgagcatggc gtcaagctgt cgggcgggca gcgccagcgt
     6301 ctctccatcg cccgcgccat gctcaagaat gcgccgatcc tgctgctcga cgaagcgacc
     6361 tccgcgctcg acacggagtc cgagcgccag gtgcaggcgg cgctcaggac attgatgcgc
     6421 ggccgcacca cgctggtcat cgctcatcgc ctctcgaccg tcgtcgacgc cgacctcatc
     6481 cacgtgatcg acggcggctg cgtcgtcgag tccgggaccc atgtcgagct gttgcgccag
     6541 aacggcatct atgcgcggct ctatgcggcg caatttgccg atcagacggc gctcagtgcc
     6601 gcgacattgc gggcggtcgg aggctgaggg tgcgagcgcc gatgaaaagg ctgcttcgca
     6661 cggcgacggc gcaccagatc ttgtgttggt tcatccatct ctacatccgc ttcgtctatg
     6721 ccaccagccg ttggaccgtc gaggggggcg acatcccgcg gcgactgcgt gacgccggcc
     6781 gtcctttcat cctcgctttt tggcatggcc ggctgctgat gatcccgatg gcctggcagc
     6841 gtctggcgcc catgcacatg ctcatctcgg gtcaccgcga cggccgcatc atcgccggcg
     6901 ccgtgcgcta cttcgatgtc gactcgatcg ccggctcttc gaatcagggc ggcaccgggg
     6961 cgcttcgcgc catggtgcgt catctcaggg acggcgattg cgtcggcatc acgcccgacg
     7021 ggccgaatgg tccggcgatg cgcgccaccc ccggcatcgt cgccgccgcc aagatctcgc
     7081 aggcgcccgt catccccatc acctatgcca cgagccggcg acggatcctg cgcagctggg
     7141 atcgctttca tctcgccttg ccgtggagcc gcggcgtcta tctctggggc gagcccatca
     7201 ccgtgccgga cgatcttggc gaaggcggcg ccgagctgtg gcgccgtcgg atcgaggatt
     7261 tcatgattgc gcagacggcc gaagccgacc gccgcgtcgg acaggaggtc atcacgcccg
     7321 gcactctgca gcgtgacgcg attcgggcgc tccgccgcgc gccccaggga gaagcgcgtt
     7381 gagctggctt cggctctatc gcgccctcac gaccgtcctc ggacccgtgg tcatggtcta
     7441 cttggcacgc cgtctggtga gcggcaagga ggaccgcgtg cgctttgccg agcggcgcgg
     7501 cgtcgccggt atcgaacggc cgcctgggcc gctcgtctgg gtccacgcag cgagcgtcgg
     7561 cgaggcggtg tcgatgctgg cgctgatcga ccggttgctg gccgagcgtc ccttgatctc
     7621 agtgatggtg acgacgggca cggtcagctc ggcacggctc ctggccgccc ggcttcccgc
     7681 gaaccgcgcc tggcatcaat acattccggt cgaccgcatt gcctatgtcc ggcgcttcct
     7741 cgaccattgg cgacccgatc tcgcgctctg ggtcgaatcc gagctatggc ccaacctcat
     7801 tctcgagacc aaggcgcgtg acatcccact gttgctgatc aatgcgcgga tgtcggcgcg
     7861 atccttccag ggatggttgc gttggcccgc gatcatcaac gaggtgctcg gcgccttcga
     7921 tctgtgcttg gcccaggacg tcgtccaggc cgagcgcctg cggcagttgg gcgcgcggcg
     7981 ttctctctgc gtcggcaatc tcaaatcggc cgcaggacct ctacccgcca gcgagtccga
     8041 gcttgtgcgg ttcgcggcac aggccggcgg ccgcccgctg tggctcgccg ccagcacgca
     8101 tccgggcgaa gaagaagctg cggccgccgt tcatcgtcgg ctgaaggcac gctggcccga
     8161 tctcctgact ctgctggtgc cgcgccaccc tgcacgcgcg gccgcggtcg ccgcgatgtt
     8221 gaagcgcgag gggctcgctg tcgcccgtcg ctcgctggcc gagccgatca cttcggccac
     8281 ggacgtctat ctcggcgata cgctgggtga gctcggactc ttctatcggc tcgccggcat
     8341 cgccttcgtc ggcggctcat tggtgcccat gggcggccac aatccgctcg aggcggcgct
     8401 ccttgattgt gccgtgctgc atgggcccga catgagcaat tgcgcttcga tcgcccgcga
     8461 tctcgcgacc gccggtgcgg ccgtcgccgc acgcgatgcc gacgagctcg ccgaagcggt
     8521 gggacggctt ctcgccgatc ccgccgagcg cgcgcgccgc gccgcggccg gcctcgaagt
     8581 ggccgccgac aaccgcgccg tcctcgatgc ggtggtggag cgcatctcgc cctggctcga
     8641 tcgcctgacg ccgcatgcca cctctgccta cgcctgagtt ctgggcgcgc cgcggctttg
     8701 tctcctctct gctccagccg ctcgcctggg cctatgccgc ggcggttgcg gcgcgttggc
     8761 agtggacgcg gggcgtcacg gcagcggtgc cggtgatctg cgtcggcaat ctcgtcgcgg
     8821 gcggcgcggg caaaacgccg gtggtgctct cgctggcacg ccggctcaag gcagcgggcc
     8881 gcgccgtcca tatcgtgagc cgcggctatg gcggccgcct cgccggcccg atcgccgtcg
     8941 atccgcggag ccatcgggcg gacgatgtcg gcgatgaggc gttgctgttg gcggaagccg
     9001 cgccgacctg ggttgcgcgc gaccgtgctg ccggagcgga gcgagcggtt gcggccggcg
     9061 cttcggtcct gctgctcgat gacggtctgc agaatcccgg gcttgccaag gatctctcgc
     9121 tcgtggtgat cgacggacaa ttcggcatcg gcaatggccg ggttctgccg gcgggtccgt
     9181 tgcgcgagcc cttcgacacc ggcgtcgccc gtgccgatgc cgtgatcctg atcggcacgg
     9241 atgacatgcg ggtcgagccg ctgctcgccg gcacgtcggt gcttcgcgcg cgtctcgtgg
     9301 ccgagaatgc cgacaggttt tgcggccatg ccgtgagcgc ttttgccggt atcggccgcc
     9361 cgacgaaatt ttttgcgacg ctcgaggctg cgggtgcccg cgtgatcgcc cgccgcgctt
     9421 tcgccgatca ccatccctat cgcgaagacg agctgcgccg cttgcaagag gaagccgtcg
     9481 gttccggcgc gacgctggtg acgaccgcca aggattgggt gcggctttcg ccggaatggc
     9541 gctcgcgtgc cgaggtactg cacgtgtcga tccggtggga agacgagttg gcgctcgagc
     9601 ggacaatggc gccggtgctc cgacactgat gagcgcacgc cgcttgttgc atttcgtcga
     9661 gcgctggatt cgcgagcgcc cggatcattg gttctggctg catcggcgct ggcccgagta
     9721 acgacgatgg gttcggatat tgcggaagtt cttgccgtcg gcggattcgc cgtcatccct
     9781 gccgcgatgc cttcaaacgt cgttacgcgc ctgcttttgc tttttgagcc ggatcccaac
     9841 gcaatgggtg gcgacgccaa gggccgttac gcgatgcgta acgtggcgga gctcataccg
     9901 gaggttcggg acgtcgcgga cggttttacc attcgctcgc tgatagatcc aatcttgggg
     9961 agagaggcgt ttctcgtccg cagccttttc ttcgacaaga ccgcgcgcgc gaattggaaa
    10021 gtggcgtggc accaggatcg ctccatcgcc gtccagcagc gaaccgaggt cgccggattt
    10081 ggcccctggt cggtaaaggc cggcgtcgct catgtcgcgc cgccggtcgc gaccctcgaa
    10141 gaaatgttga caatgcgcct gcacctcgac gattgcgatg agagcaacgg cccgttgcgc
    10201 gtgattcccg gctcgcatct gaaaggcagg ctcacggcga ggcaaatccg cgacgagatc
    10261 gctgaacggc cgcaggtgtc gtgtccggtc gctgcgggcg gcgtcttgct catgcggccc
    10321 ctactgttgc acgcttcgtc accagcgcga ccgccgcgtc atcgccgcgt ggtgcatctg
    10381 gagttcgcgc gctcccgttt gcccgatgga ctgcgctggg cctgcgaggc ccggtgatcg
    10441 gggagtaggc cgctcatagc gatcatgacg agcggcgggt cagtgccccg cttgcggggc
    10501 cgtggtctgc ggcatcggac ccacagcgag tgccgggtcg aggcgcacct cataccagtt
    10561 gagagcccaa tggagatgcg ggccggtgac gcggccggtg gcaccgacga tgccgatggg
    10621 ctcgcctgcc tccacatgct gccccagctt cacgtcgacg cggtccatgt gctgataggt
    10681 cgtggcgagc ccgtagccat ggtcgatgat gaccgtgccg ccggtgaaga agagatcgcg
    10741 ctcggcaagc gtcacggtcc cggcggcggc cgcagccacg ggtgagccac gcggtgccgc
    10801 gacgtcgacg ccggcatggg gggcgcgcgc ctcgccgttg aggatacgct gactgccata
    10861 gacgccggag atgggaccga gcgccggcca gcgcagcggt tcttcgaaga agagcaggtc
    10921 ggtgtcggcg gcacgggccc gcttgatgat ctcgccttcg cgcgcgacgc gctccatctg
    10981 cgcggcatcc ggcgtcaccg tctgctgcgg cagtccttcg atgcgttgga tgtcgtagct
    11041 gcgcgcggtc acgctgagat cgcgatgctc gtgaccgcca tcggggaagg cgacgtcaag
    11101 cgacgcatgg ttgggcgcat cgcggccgaa gccgaaaatg aagctgccat cgggcgcgac
    11161 gcggaccgcg cggccgtcga gcgtgaccgt ggcaccggga tcggccttgc cgcgaacgag
    11221 agcgccttgc tctaccgtgc cgtcgagcga gagcgtgccg gccgtgcaag gagtc
//
LOCUS       DASYNS010000478         5434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5113037, whole genome shotgun sequence.
ACCESSION   DASYNS010000478 DASYNS010000000
VERSION     DASYNS010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5434
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5113037"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3046
                     /locus_tag="VGP50_14875"
     CDS             <1..3046
                     /locus_tag="VGP50_14875"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEV8028715.1"
                     /translation="LPVLEHLGLRVIAEIPYAVAAIGEPAASLQDFSLTVPSVDVDVA
                     RDRARFEEAFLRLWAGAMESDGLNRLVLAAGLDWRQVVMLRLYVKVLRQAGSAYSQAY
                     MEDALAAHAVIARQLAQLFERRFNPERDRGRDAETAKLVQGIERALEDVASLDEDRIL
                     RSMLLLVTKSLRTNYFQRDAGGEAKPYLAVKLASREIDLLPLPRPLCEIYVLSPRVEG
                     VHLRGGKVARGGIRWSDRKEDFRTEILGLMKAQMVKNAVIVPTGSKGGFVVKRPPASR
                     DALQAEGVECYKTLLRGLLDLTDNIVGAKIVPPRDVVRHDGDDPYLVVAADKGTATFS
                     DIANGVSADYGFWLGDAFASGGSAGYDHKVMGITARGAWETVKRHFRERGTDVQTTDF
                     TVVGVGDMSGDVFGNGMLRSEHIKLIAAFDHRHIFLDPDPDPGVSFAERKRLFDLPRS
                     SWADYDKKLISAGGGVFERTRKSIALSPETRARFALAAEKMTPAELIQALLKAPVDLL
                     WFGGIGTYVKAREERNAEVGDRANDGLRVDAEDVAAAVVGEGANLAVTQRGRVGYALK
                     GGRINTDAIDNSAGVDTSDHEVNIKILLDAVVAAGDLTQKQRNALLQEMTDAVANLVL
                     ADNDLQGLALSLAEAQGLSQLDEQQRLMRRLERAGELARAVEFLPDDEALAARAATGR
                     GLTRPELAVLMAYVKNTLADALVESDLPADPALEQDLTAYFPPVLVERFRPAIAAHRL
                     RREIIATVAANDLVNRGGITLLTEMVERTGRSEGDVARAYAIAREILSLDALWQAVSA
                     LDNRVAARVQHQILLSGQRLLRRVTAWFLRGHTPLDIVAQIAAFRPGVQELAARIEEI
                     LPGTERAQFARNRDMLVADGVPGDVALAAARLDFLTSSVDIVRLGAAGSVGVAEIARR
                     FYAVGARFGLDSLRAFARRLKAETTWQKLAIDAVIEDFYAQQAELAAQAIAADALEPW
                     LDARVAPLARLDALLREIDTAPAPDLAMLTVAARALRSATAG"
     gene            3102..3503
                     /locus_tag="VGP50_14880"
     CDS             3102..3503
                     /locus_tag="VGP50_14880"
                     /inference="COORDINATES: protein motif:HMM:NF021510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 domain-containing protein"
                     /protein_id="HEV8028716.1"
                     /translation="MPKDGNGNGGRQPSRNVLGERLESCSFDPVTGFFRNGCCDTSAE
                     DLGSHTVCVVMTTEFLAFSKARGNDLSTPMPEFGFAGLKAGDRWCLCAPRWQEALEAG
                     HAPRVALRATHEGALRYCTLADLKRHAADLS"
     gene            complement(3518..3880)
                     /locus_tag="VGP50_14885"
     CDS             complement(3518..3880)
                     /locus_tag="VGP50_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028717.1"
                     /translation="MIAAAFLGGGLLHVRQSPGQAIRTELAAAAADMTSAPANLPPDE
                     LKRDVKRHFLGQVVTIDPAGFPAQVAVTIHGLDRDACIDAASVARRIEGLAVIELEGY
                     RSADDCGKRNDMRWQILP"
     gene            4124..4927
                     /locus_tag="VGP50_14890"
     CDS             4124..4927
                     /locus_tag="VGP50_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028718.1"
                     /translation="MARIDFSSALGDAFAFTANNLGRLFRLCGLWLALIIAFQLFALF
                     MPGIMARGTMMAAVSRGLYVCFYLAVSVMFAVAWHRAVLRSDGPRTMMDMMHFGRREW
                     RFLGYLILFAATVCLPLIGAVTTTGMFIVTRSQGIGIVMTVCWLLSLLVWLAISRLSL
                     ALPAAAVDESGGGLGIAWQRGKGNMMGLFFGPIVCAVPFVILSFVLYLVFAALATVSP
                     AFDALRVVIIAALGFVQAGFGVSFLSFCYRQVASTGVATPRRMAAMPAE"
     gene            5110..5241
                     /locus_tag="VGP50_14895"
     CDS             5110..5241
                     /locus_tag="VGP50_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028719.1"
                     /translation="MQDFVRDWRRWRVEERIVAALVFGFVIGAPFVPLVVAIVTTPR"
     gene            complement(5263..5421)
                     /locus_tag="VGP50_14900"
     CDS             complement(5263..5421)
                     /locus_tag="VGP50_14900"
                     /inference="COORDINATES: protein motif:HMM:NF040443.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028720.1"
                     /translation="MQDQLMSDLQERLRKNIQAMDPETMIRTWLPATIKSFEQWQSFL
                     AQLGAKKT"
BASE COUNT          799 a         1842 c         1833 g          960 t
ORIGIN      
        1 gttgccggtc cttgagcatc tggggctcag ggtcatcgcc gaaatcccct acgccgtcgc
       61 cgccattggc gagcccgcgg cgtcgcttca ggatttcagc ttgacggtgc cgtcggtcga
      121 cgtcgacgtg gcgcgcgacc gcgcccgttt cgaggaggcc ttcctccggc tctgggcggg
      181 cgccatggag agcgacggcc tcaaccgcct ggtgctcgcc gccggcctcg actggcggca
      241 ggtggtgatg ctgcggctct acgtgaaagt gctgcgccag gccggcagcg cgtacagcca
      301 ggcctatatg gaggacgcgc tcgcggccca tgcggtgatc gcgcgccagc tcgcccagct
      361 gttcgagcgg cgcttcaacc ctgagcgcga tcgcggccgc gacgccgaga cggcgaagct
      421 ggtgcagggc atcgagcgcg cgctcgagga cgtcgcgagt ctcgacgagg accgcatcct
      481 ccgcagcatg ctgctgctcg tgacgaagtc gctgcgcacc aactacttcc agcgcgacgc
      541 gggcggcgag gcgaagccct atctcgccgt gaagctggcg agccgcgaga tcgatctgct
      601 gccgctgccg cggccgctct gtgagatcta cgtgctgagc cccagggtcg agggcgtgca
      661 tttgcgcggc ggcaaggtgg cgcgcggcgg catccgctgg tccgatcgca aggaggattt
      721 ccgcacggag atcctgggcc tgatgaaggc gcagatggtc aagaacgcgg tgatcgtgcc
      781 gacgggctcc aaaggcggtt tcgtggtaaa gcgaccgccc gcgtcgcgcg acgcgctcca
      841 ggccgaaggc gtcgaatgct acaagaccct gctgcgcggc ctcctcgatc tcacggacaa
      901 catcgtcgga gcgaagatcg tgccgccgcg cgacgtcgtc cgtcatgacg gcgatgatcc
      961 ctatctcgtc gtcgccgccg acaagggaac cgcgacgttc tccgacatcg ccaatggggt
     1021 gagcgccgac tatggcttct ggctcggcga tgcgttcgcc tccggcggct cggccggcta
     1081 cgaccacaag gtcatgggca tcaccgcccg gggcgcgtgg gagacggtga agcgccattt
     1141 ccgcgagcgc ggcaccgacg tccagacgac ggatttcacc gtggtcggcg tcggcgacat
     1201 gtcgggtgat gtcttcggca acggcatgct gcgctcggag catatcaagc tcatcgccgc
     1261 cttcgatcat cgccacatct ttctcgatcc cgaccccgat cccggggtga gcttcgccga
     1321 gcgcaagcgt ctcttcgatc tgccgcgctc gagctgggcg gattacgaca agaagctgat
     1381 ctcggcgggc ggcggggtct tcgagcgcac gcgcaaatcg attgcccttt cgccggagac
     1441 gcgcgcgcgt ttcgctcttg ccgccgagaa gatgacgccc gccgagctga tccaggcact
     1501 gctcaaggcg ccggtggacc tgctgtggtt cggcggcatc ggcacgtatg tgaaagcgcg
     1561 cgaggagcgc aacgccgagg tcggcgaccg tgccaatgac ggattgcgcg tcgacgccga
     1621 ggatgtcgcc gcggcggtcg tcggcgaggg cgcgaatctc gcggtgacgc agcgcggccg
     1681 ggtcggctac gcgctcaagg gcggccgcat caataccgac gcgatcgaca actcggccgg
     1741 tgtcgacact tcggatcacg aggtcaacat caagatcctg ctcgacgccg tggtcgccgc
     1801 cggcgacctg acgcagaagc agcgaaatgc cctgcttcag gagatgaccg acgcggtcgc
     1861 gaatctcgtg ctcgccgaca atgacctcca ggggctggcg ctgagcctcg ccgaagcgca
     1921 ggggctgtcg cagctcgacg agcagcagcg gctgatgcgc cgtctcgaac gagcgggcga
     1981 gctcgcgcgc gccgtcgagt tcctgccgga cgacgaggcg ctggccgcgc gcgcggcgac
     2041 gggccgcggc ctcacccggc ccgagctcgc cgtgctgatg gcctatgtga agaacacgct
     2101 cgccgacgcg cttgtcgaat cggatctgcc ggcggatccg gcgctcgagc aggatctcac
     2161 ggcctatttc ccgccggtgc tggtcgagcg ctttcgcccg gccatcgccg cgcaccggct
     2221 ccgccgcgag atcatcgcca cggtcgcggc caacgacctc gtcaaccgcg gcgggatcac
     2281 cttgctcacc gagatggtcg agcgcaccgg gcgcagcgaa ggcgacgtcg cccgcgccta
     2341 tgcgatcgct cgcgagatcc tctcgctcga cgcgctctgg caggccgtca gcgctctcga
     2401 taatcgcgtc gcggcccggg tccagcacca gatactgctc agcggacagc gactcctcag
     2461 gcgcgtcacc gcctggtttc tccgcgggca tacgccgctc gacatcgttg cccagatcgc
     2521 cgccttccgt cccggcgtcc aagagctcgc cgcgcggatc gaagagatcc tgccggggac
     2581 ggagcgtgcc cagttcgcgc gcaaccgcga catgctcgtc gccgatggcg tccccggcga
     2641 tgtcgcgctg gcggcggcgc gtctcgattt cctgacctct tccgtcgaca tcgtgcgcct
     2701 cggtgccgcg ggaagcgtcg gcgtcgccga aatcgcccgg cgcttctacg cggtgggcgc
     2761 gcgtttcggg ctcgactcat tgcgcgcctt cgcgcgccgc ctcaaggcgg agacgacctg
     2821 gcagaagctc gcgatcgacg cggtcatcga ggatttctat gctcagcagg ccgaactggc
     2881 ggcccaggcg atcgccgccg atgcgctcga gccctggctc gacgcccgcg tcgcgccgct
     2941 cgcgcgcctc gatgccctgc tgcgcgagat cgacaccgct ccggcgcccg acctcgccat
     3001 gctcaccgtc gccgcccgtg cgctgcggag cgcgacggcg ggctaggcga gcttcaccgc
     3061 acgcgctatg tcttcagccg agtgaacccc gggaggaggc gatgccgaag gatggcaatg
     3121 gaaatggcgg ccgacagccg tcgcggaacg tgctgggcga gcgtctcgag agctgctcgt
     3181 tcgatccggt gaccggcttc ttccgcaatg gctgttgcga cacctcggcc gaagacctgg
     3241 gcagccacac cgtgtgcgtc gtcatgacca cggagttcct cgccttctcc aaggcgcgcg
     3301 gcaacgacct ctcgacgccc atgccggaat tcgggtttgc cgggctgaag gccggcgacc
     3361 gctggtgtct ctgcgcgccg cgctggcagg aggcgctcga agccggccat gcgccgcgcg
     3421 tggcgctgcg cgcaacccat gaaggcgctc tgcgctattg cacgctcgcc gacctcaagc
     3481 gccacgccgc cgatctcagc taggctccta gagcgcatca gggcaggatc tgccagcgca
     3541 tgtcgttgcg cttgccgcag tcatcggccg agcgatagcc ctcgagctcg atgaccgcga
     3601 ggccttcgat gcggcgcgcg acgctcgcgg catcgatgca ggcgtcgcgg tccagtccat
     3661 ggattgtcac ggccacctgc gcgggaaagc cggcgggatc gatcgtcacc acctgtccga
     3721 ggaaatggcg cttgacgtcg cgcttcagct cgtcgggcgg caggtttgcc ggtgccgacg
     3781 tcatgtcggc cgccgccgcg gcgagctcgg tgcggatggc ctggccgggc gattgccgga
     3841 cgtgcaagag gccgccgccg agaaaggccg cggcaatcaa cccgaatgcg ccggaggcgc
     3901 ggacggttcg tctctgtaat atcagtatcc ggaaaggcac tgctgcatcc atggcgaaca
     3961 ggctcaggga tgccggagaa ttttgccgaa attgtggcga gtttccgcag ctattcggac
     4021 atggtttaac caaatgtccg gcgaaacgcc ttgctcgcgc tcgcctcagg cgagcatcat
     4081 ccgccctctt tccgtgtcgg cgcaaaaaaa gggggagccg cgcatggcca ggatcgattt
     4141 ctcgtcggcg cttggcgatg ccttcgcgtt caccgccaac aatctcggtc ggctcttccg
     4201 tctttgcggc ctctggctcg cgctgatcat cgcgttccag ctgttcgcgc tcttcatgcc
     4261 cgggatcatg gcgcgtggca cgatgatggc cgcagtgagt cgcggccttt atgtctgctt
     4321 ctatctcgcc gtctcggtga tgttcgccgt cgcctggcac cgcgccgtgc tgaggagcga
     4381 cgggccgcgc acgatgatgg acatgatgca tttcggcaga cgcgagtggc ggttcctcgg
     4441 ctatttgatc ctgttcgcgg ccactgtctg cctgccgctc atcggggcgg tcacgaccac
     4501 cgggatgttc atcgtgacgc gcagccaagg cattggcatc gtcatgacgg tctgctggct
     4561 gctgtcgctc ctcgtctggc tcgcgatctc ccgcctctcg ctggcgctgc cggcggcggc
     4621 cgtcgatgag agcggcggcg gcctcgggat agcgtggcag cgcggcaagg gcaacatgat
     4681 gggcttgttc ttcgggccga tcgtctgcgc cgtgccgttc gtcatcctct ctttcgtgct
     4741 ctatctcgtc tttgccgcac tcgccacggt cagccccgcc ttcgacgcgc tgcgcgtggt
     4801 gatcatcgcc gcgctcggct tcgtgcaggc cggcttcggg gtgagcttcc tctcgttctg
     4861 ctaccggcag gtggcgagca cgggcgtggc gacgccgcgg cgcatggccg cgatgccggc
     4921 ggaataataa tctccgaagc ttagatctcg cttgccgcgc gccatcagcg ttgcggcaag
     4981 cggcctgctg actgtcatta actcagcttc gccaacgcgc gcgatgtgaa gtcgcccaca
     5041 tccggatggg caggcgaatg gcacgctctc ggcggtgacg atggaccgca ctttgccggg
     5101 gctcgcgcga tgcaggactt tgtgagggat tggcgccgtt ggcgtgtcga agagcggatc
     5161 gtcgccgcgc tggtcttcgg tttcgtcatc ggcgcgccct tcgtaccgct ggtcgtcgcc
     5221 atcgtcacga ctccgcgctg agcgcggccg cggctccgcc ggtcaggttt tctttgcgcc
     5281 gagctgcgcg agaaaactct gccactgctc gaagcttttg atggtcgccg gcagccaggt
     5341 ccggatcatg gtctccgggt ccatcgcctg gatgttcttg cgcagccgct cctggagatc
     5401 gctcatcagc tgatcctgca tcggcttcac gtcg
//
LOCUS       DASYNS010000479         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1526357, whole genome shotgun sequence.
ACCESSION   DASYNS010000479 DASYNS010000000
VERSION     DASYNS010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526357"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1351)
                     /locus_tag="VGP50_14905"
     CDS             complement(224..1351)
                     /locus_tag="VGP50_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV8028721.1"
                     /translation="MHSLDAFASEKLDRLERENLRRVVVPSVREEGIWVRRGGRRLLS
                     FSCNDYLNLTQHPRVKAAAIAAIEQYGTGAGASRLVTGDHPLYAVLEERLARLKGSDA
                     ACVFGSGYLANAGIVPALVGKDDAVLIDALAHSCLWAGALLSGAAVLSFRHNDLGHLE
                     DLLAAERGRFRHVLLATDHVFSMDGDRAPLDALAALAGRFDAWLMADDAHGLGVVAGA
                     KAPVPLQMGTLSKAVGGYGGYLCASRPVIDLVKTRARTLVYSTGLPPATVAAAIAALA
                     IIEESPALVAQPLAKARLFTARLGLAPAESAIVPILLGAPETALAASGTLAEEGFLVT
                     AIRPPTVPDGTARLRLAFTAGHPEEEILRLADVVEQLRGKE"
     gene            1601..>2127
                     /locus_tag="VGP50_14910"
     CDS             1601..>2127
                     /locus_tag="VGP50_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010507861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV8028722.1"
                     /translation="MSWLDEGLPHVWLPYAQMKTAAPPLAVARTDGTRIHLADGRVLV
                     DGVASWWTACHGYNHPHIRDAVRRQLELMPHVMFGGLASEPALTLARRLSALLPPGLE
                     RVFFSDSGSVAVEVAVKMAVQYWLNRGVRGRTRLLAFSGAYHGDTFATMALCDPEDGM
                     HRLFAGLLPQHVIVNL"
BASE COUNT          328 a          783 c          705 g          311 t
ORIGIN      
        1 gcctttgtcg cagggctccg acccagccag ttacccagcc gagccgcctg ccagctaccg
       61 ggccaaccga ccattgcccg ggtgggactt ccacccacaa ggtgatcgcg cccttcgggg
      121 cgcaccagag gacgcggagg agaaaagtat ccctctgcgt cctccgcggc gcctccgcgc
      181 cctctgcgtt cttctttttc tctgtgtctc tgtgcctctg tggtcactct tttcctcgca
      241 actgctcgac aacatcggca aggcgcagga tctcctcctc cggatgccct gccgtaaatg
      301 cgagacgcag ccgcgccgtc ccgtccggca cggttggcgg tcggatcgcc gtcacgagaa
      361 agccctcctc cgccagtgtc cccgacgccg cgagtgctgt ttccggcgcg cccagcagga
      421 tcggcacgat cgcgctctcg gccggcgcca ggccgagccg cgccgtgaag agccgcgctt
      481 tcgctaaggg ctgcgcgacg agcgccgggc tctcttcgat gatcgcgagc gcggcgatgg
      541 ccgcggcgac ggtggccggc ggcaagcccg tcgagtagac gagcgtgcgc gcccgcgtct
      601 tgacgagatc gatcaccggc cgcgacgcgc agagatagcc gccatagccg ccgaccgcct
      661 tcgacagcgt gcccatctgc aagggcacag gggccttggc cccggcgacg acgccgaggc
      721 catgcgcgtc atcggccatg agccaggcgt cgaagcggcc ggcgagcgcc gccagcgcgt
      781 cgagcggcgc gcggtcgcca tccatcgaga agacatggtc ggtggccagc agcacatggc
      841 ggaagcgccc gcgctccgcc gccaggagat cctcgagatg gccgagatcg ttgtggcgga
      901 agctcagcac cgcggcgccc gagagaagcg cgcccgccca gagacaggag tgggccagcg
      961 cgtcgatcag caccgcatcg tccttgccga caagcgccgg gacgatgccg gcattggcga
     1021 ggtatcccga gccgaagacg caggcggcgt cgctgccctt gagccgcgcc agcctttcct
     1081 cgagcacggc atagagcgga tgatcgccgg tgacgagccg cgacgcgccg gcgccggtgc
     1141 catactgctc gatcgccgcg atcgccgccg ccttgacgcg cggatgctgc gtcaggttga
     1201 ggtagtcgtt gcacgagaac gacaagagcc gccgcccccc gcgccgcacc cagattccct
     1261 cctcccgcac gctcggcacg accacgcgac ggagattctc ccgctccaat cggtcgagtt
     1321 tctcgctggc gaaagcgtcg agcgaatgca tgcagcttcc ggaaccaaat gcggtgcagt
     1381 agagaccccc tctcccctca cgggagaggg aggggcccgg cgcgaagcgc cgggagggtg
     1441 agggggctgg cgacgagatg tctctacgca gatcgaagcg gcggaaccgc cccctcaccc
     1501 tcccgccgct gacgcggcgg gcccctccct ctcccgcgag gggagaggga gttgacgtcc
     1561 ttagccccaa catcgccacc gcacaactcg gggcccgcgc atgagctggc tcgacgaagg
     1621 gctgccgcat gtctggctgc cctatgcgca gatgaagacg gcggcgccgc cgctggcggt
     1681 ggcgcggacc gacggcacgc gcatccatct cgccgatggg cgcgtgctcg tcgacggcgt
     1741 cgccagctgg tggacggcct gccatggcta caaccatccg catatccgcg acgccgtgcg
     1801 gcgccaactc gagctcatgc cgcatgtcat gttcggcggc ctcgccagcg agccggcgct
     1861 gaccctggcg cggcgcctgt ccgcgctgct gccaccgggg ctcgagcgcg tgttcttctc
     1921 tgacagcggc tcggtcgcgg tcgaggtcgc ggtcaagatg gccgtgcaat actggctcaa
     1981 ccgcggcgtg cgcggccgga cgcgcctcct cgccttcagc ggcgcctatc acggcgacac
     2041 cttcgccacc atggcgctct gcgatcccga ggacggcatg caccgcctct tcgccggcct
     2101 cctgccgcag cacgtcatcg tcaacct
//
LOCUS       DASYNS010000480         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1526422, whole genome shotgun sequence.
ACCESSION   DASYNS010000480 DASYNS010000000
VERSION     DASYNS010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526422"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..622)
                     /locus_tag="VGP50_14915"
     CDS             complement(197..622)
                     /locus_tag="VGP50_14915"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV8028723.1"
                     /translation="MRGSERRRHARRSGLWAAQLETAQGARRNCLVLDVSQSGAKLRT
                     EQTTAPGEIVTLIDERSGTRRGRVVWAGNHRVGISFVDPSGAAAKTQTPASDPKFLRG
                     RAEVLRRLARSAQGGDAAAGLLRLAQSLEAEAREIEKQR"
     gene            complement(694..1263)
                     /locus_tag="VGP50_14920"
     CDS             complement(694..1263)
                     /locus_tag="VGP50_14920"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV8028724.1"
                     /translation="MYLRHHGKRSPAGPRRVDDDAVGILTERRRHRRRAGAWTAIVET
                     THGERIGSVVLDLSPGGAKLLLERPIAVGELVSFLTERFGTHRGRIAWVSGVRVGLAF
                     ANFAVGAASKALADKPEFLRGRAEVLRRLAQTARSGETVSGLLWAAKGFDEAADRIEA
                     QQRWPSTSPSPSIRKEDILFGRPVLPPPR"
     gene            complement(1382..>1729)
                     /locus_tag="VGP50_14925"
     CDS             complement(1382..>1729)
                     /locus_tag="VGP50_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEV8028725.1"
                     /translation="AFHDGDEELVPGLSVHHIGGHTAGIQSVRVLTRRGFVVLASDAS
                     HLYANMEEGRPFPIVYNVADMLEGHRRLQRLAASPRHVVPGHDPLVMRRYPPPSRALE
                     GVAVRLDAEPLSR"
BASE COUNT          285 a          621 c          566 g          257 t
ORIGIN      
        1 gcctttgtcg cagggctccg acccagccag ttacccagcc gagccgcctg ccagctaccg
       61 ggccaaccga ccattgcccg ggtgggactt ccacccacaa ggtgatcgcg cccttcgggg
      121 cgcaccagag gacgcggagg aacgactatc ctctgcgtcc cccgcgaaac ctctgcgccc
      181 tctgcgttcc gctacactag cgctgttttt cgatctcgcg cgcctcggct tcgagcgatt
      241 gggcaaggcg cagaagaccg gcggcagcat cgccgccttg ggcgctacgc gccagccggc
      301 gcaggacttc ggcgcggccg cgcaggaatt tgggatcgga ggccggcgtc tgcgtcttcg
      361 ccgctgcgcc ggacgggtcg acgaaagaga tgccgacgcg gtgattgccg gcccacacga
      421 cgcggccgcg ccgcgtgccg gagcgctcgt cgatcaaggt cacgatctcg ccgggcgccg
      481 tcgtctgctc tgtccgcagc ttcgccccgc tctgcgacac gtcgaggacg aggcagttgc
      541 gccgcgcacc ctgcgctgtc tcgagctgcg ctgcccagag accgctgcgc cgcgcatggc
      601 gccggcgttc gctgccgcgc acggcagcgc tgcgaaacgc gctctgaggc tcacctccgt
      661 caatgccatc cacgatatga ccccccgtca tgctcaacgc ggcggcggaa gaaccggccg
      721 gccgaacaga atgtcttcct tgcggatcga cggcgacggc gaggtcgacg gccagcgttg
      781 ctgcgcctcg atgcgatctg ccgcctcgtc gaagcccttc gccgcccaca acagacccga
      841 caccgtctcg ccgctgcgcg ccgtctgcgc caggcgtcgc aacacctcgg cgcggccacg
      901 caggaattcg ggcttgtcgg caagcgcttt gcttgccgct ccgacggcga aattggcaaa
      961 ggcgagcccg acgcgcaccc ccgacaccca ggcgatgcgc ccgcgatgcg tgccgaagcg
     1021 ctccgtcagg aagctcacga gctcgccgac ggcgatgggc cgctccagca gcagcttggc
     1081 gccgcccggc gaaagatcga ggacgacgct gccgatgcgc tcgccatggg tcgtctcgac
     1141 aatagccgtc cacgcgcccg cgcgccgccg gtggcgacgg cgctcggtca ggatgccgac
     1201 ggcatcgtca tcgacccggc gggggccggc cggactgcgt ttaccatggt gccgcaaata
     1261 catggggcga cacgcctcgc cgcttgagta acttctcgga aagcatactg ctccgggcaa
     1321 tcggcgccac ggattttact taaaatccga tggaattttc accaaatcac cgcgacgact
     1381 ttcatcgcga caacggctcg gcatcgaggc gcacggcgac gccttcgagc gcgcgcgacg
     1441 gcggcggata gcgccgcatc accaacggat cgtgtcccgg cacgacgtgc cgcggcgatg
     1501 cggcgagacg ctgcagccgg cggtgaccct cgagcatgtc ggcgacgttg tagacaatgg
     1561 ggaagggccg cccctcctcc atgtttgcgt aaaggtggct cgcatcggag gcaagcacga
     1621 cgaagccgcg ccgtgtcagc acgcgcaccg actgaatgcc ggcggtgtgc ccgccgatgt
     1681 gatggacgga aaggccgggg accagttcct cgtcgccgtc atggaacgc
//
LOCUS       DASYNS010000481         7423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1211930, whole genome shotgun sequence.
ACCESSION   DASYNS010000481 DASYNS010000000
VERSION     DASYNS010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211930"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VGP50_14930"
     CDS             <1..745
                     /locus_tag="VGP50_14930"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8028726.1"
                     /translation="SLVTYLALNQRNPYLRRPAVVEAIEYLVDYDGIAHAILGPTAAI
                     HQGFEPEGILGAIDDRPFRYDPARARALLSEAGLAGGFAVTIDVRNTSPGSDVAQALQ
                     ASFAAAGITLGIIAADDKQVLTKYRARHHDIFLGEWGPDYPDPHSNAEAFAVNDDNAD
                     GAARKTPAWRTAWSDPAMNAAVAAAMTERDVVRRAQLYQDLQRAHQRAAPFVFLFQRV
                     EIAAHRADIDGLVLGLASDQTRYAGIAKR"
     gene            935..1954
                     /locus_tag="VGP50_14935"
     CDS             935..1954
                     /locus_tag="VGP50_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8028727.1"
                     /translation="MSMTRYFATTLRVLAQVLLTLLGLLLATFLIARVVPIDPVLAVV
                     GDRASSETYARVRSELGLDRPLYVQFYRYVATASRGDFGRSVLTRNLVTDDIARFLPA
                     TIELATLATLLGVGLGLPLGVVAAARHGRWPDHLIRFVALAGYSVPVFWLGLMALLLF
                     YAGLGWAAGPGRLDIYLEDSIRPVTGLLLVDSVIAGDWGVLRSALAHLVLPASVLGYF
                     ALAYVSRMTRSLMLDQLGQEYIVTARAKGLSERRVLWRHALGNVMLPLITVVALSYAS
                     LLEGSVLTETVFAWPGIGSYITSSLLAIDMNAVLGGTLVVGTIFIAVNLLSDLLYRWL
                     DPRAR"
     gene            1951..2853
                     /locus_tag="VGP50_14940"
     CDS             1951..2853
                     /locus_tag="VGP50_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8028728.1"
                     /translation="MRAAAWHDWLTAEAPGSARQARLAEAYRLLRAFARHRAGMTGLV
                     IVLLLVAAAILAPLLARFSPSAQDLALRLKPPDAVHWLGTDELSRDIFSRLLYGARVT
                     LSIVALVTAISAPLGLAIGCTAGYAGGILDAGLMRLTDVFLSLPRLILALALVAALGP
                     GIGNAALAMALVAWPPYARVARAETLALRGSDFIAAARLAGASPARIVLRHIMPLCLA
                     SVIVRVSLDMAGVILIAAGLGFLGLGAQPPTPEWGAMIAAGRRYMLDQWWVAAMPGLA
                     IAVASLGFNLLGDGLRDVLDPKQR"
     gene            2850..3638
                     /locus_tag="VGP50_14945"
     CDS             2850..3638
                     /locus_tag="VGP50_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020594470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028729.1"
                     /translation="MSAALLEIAELRLGFGGDRPFEALSGVGFTMGRERLGIVGESGS
                     GKSLTGRSILRLPPPDAVMSARRLSFDGIDLLTAGEDALRALRGRRIGLVLQDPRFSL
                     NPVMAVGRQIAEAYRAHGRAGAREAREKALAMLEAVRIREPGRVFDLYPHQISGGMGQ
                     RVMIAMMLAPGPDLLIADEPTSALDVTVAIDVLSILDEEVSRRGMGLVFISHDLDLVA
                     GFCDRVLVMERGRIVESCEAAALESAEHPYTRRLLASRPRLRAS"
     gene            3635..4375
                     /locus_tag="VGP50_14950"
     CDS             3635..4375
                     /locus_tag="VGP50_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028730.1"
                     /translation="MIEASALTARFGAVTAVDQVSFTVAGGEAFGIVGESGSGKSTLL
                     RVLAGLHEDWSGRLAVGGAARVAKRGAAMSGAVQMVFQDPYGSLHPRHSVDRTLGEPL
                     AIHRMDGAAARIAAALDAVGLERAQRFRYPHQLSGGQRQRVAIARALILEPKLVLLDE
                     PTSALDVTVQAEILSLLRRLRRERGLTYVLVSHNLAIVAELCDRVAVMRDGRFVEELS
                     AAALAAGEARHPYTQELLRASRGYRRQR"
     gene            complement(4379..4672)
                     /locus_tag="VGP50_14955"
     CDS             complement(4379..4672)
                     /locus_tag="VGP50_14955"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV8028731.1"
                     /translation="MLPVVFGPGARAEMLEASHWYATHAEVLARRFVGEVDAVVTRIA
                     DNPLQFPLVLKDVRRAKLRRFPYALFFRVVDETVFVIACFHARRDPQRWHGRV"
     gene            complement(4672..4890)
                     /locus_tag="VGP50_14960"
     CDS             complement(4672..4890)
                     /locus_tag="VGP50_14960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEV8028732.1"
                     /translation="MKDRPLDDIARLSPQQRLALISELWDSLTDADVPLTPAQEAELE
                     HRLARFEADRAQGVPWEQLKAELPTRRP"
     gene            complement(4927..5091)
                     /locus_tag="VGP50_14965"
     CDS             complement(4927..5091)
                     /locus_tag="VGP50_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028733.1"
                     /translation="MRVAETIAGELMPIHCCLTSLGGTTKPWRCFAATAAPGGIHGLA
                     AGHEGANGFG"
     gene            complement(5088..5600)
                     /locus_tag="VGP50_14970"
     CDS             complement(5088..5600)
                     /locus_tag="VGP50_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028734.1"
                     /translation="MIMSFVDGGEEKKKKDDTSEKEYGFKNIACHVWAIDGYKDETTA
                     KSVNSRYNNSANRLKVANNEASRNEALKTLVGVARVSETTGEQIIVYGGFRRGQNAPE
                     HMWMEYKNKIYETMPDYDLWVADADAKTRNCPPLENAPFPSGGVAKYATFLTVNQRDY
                     LTKAGHLKPG"
     gene            complement(5784..6446)
                     /locus_tag="VGP50_14975"
     CDS             complement(5784..6446)
                     /locus_tag="VGP50_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV8028735.1"
                     /translation="MPSTAASSSAASPVDDDKAVQILCREIAYQGFFRIERYRVRHRL
                     HAGGWSPELEREVFERGAAVAVLLYDPERDAVVLVEQFRLAAHLAGFPAWQVEVVAGI
                     VDRDGEAEPDVARREAQEEAGLAIQGELVPIHRCLTSPGGTTETVALFCGRIDSRNAG
                     GIHGVAAEHEDIKVVVKSYRDAMRLMRAQKIDNAFTLLALHWLAANRATLRRRWRTAR
                     PA"
     gene            complement(6505..6897)
                     /locus_tag="VGP50_14980"
     CDS             complement(6505..6897)
                     /locus_tag="VGP50_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028736.1"
                     /translation="MELAKLVSTLQSAQDGSEYLDYAIQRLFGTKKPVPLYTRSLDAA
                     LTLVPEGWTIHRLGQMSNCQGGFGGWIGEIYRARDAMIPYPSSAPAASAPLAICVAAM
                     RVRLAEQLEAETARNVSTVADRVQRSGT"
     gene            complement(6979..>7423)
                     /locus_tag="VGP50_14985"
     CDS             complement(6979..>7423)
                     /locus_tag="VGP50_14985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028737.1"
                     /translation="VERRLRLKGHIAPHVLVHANAEMVETVIENLLDNAVSFSPEGES
                     IGVRLETVGGAAELLIGDSGPGVPPENLSRIFDRYFSQRTPPPNGESQGPHFGIGLWI
                     ARRNVEALGGTITAENRRPNGLLMRVRLSRAEVARLVSIPTKART"
BASE COUNT         1108 a         2523 c         2483 g         1309 t
ORIGIN      
        1 aagcctcgtc acctatctcg cgctcaatca acgcaatccc tatttgcggc ggcccgcggt
       61 ggtcgaagcc atcgaatacc tcgtcgacta tgacggcatc gcgcacgcga tcctcggccc
      121 gaccgcggcc atccaccagg gcttcgagcc cgagggcatc ctcggcgcga tcgacgatcg
      181 acccttccgc tacgatcccg cgcgtgcccg ggcgctgctt tccgaggccg gactcgccgg
      241 cggcttcgcc gtcaccatcg atgtgcgcaa cacctcgccc gggagcgacg tggcgcaggc
      301 gcttcaggcg agcttcgccg ccgccggcat cacgctcgga atcatcgccg ccgacgacaa
      361 gcaggtgctg accaagtacc gggcgcgtca tcacgacatc tttctcggcg aatggggtcc
      421 ggattatccc gatccccaca gcaatgccga agcctttgcc gtcaacgacg acaacgcgga
      481 cggcgcggcg cggaagacgc cggcctggcg cacggcctgg tccgatccgg cgatgaacgc
      541 cgcggtcgcc gccgccatga cggagcgcga tgtcgtgcgg cgcgcgcagc tctatcagga
      601 tctgcaacga gcgcatcaac gcgcggcacc cttcgtcttc ctgttccagc gcgtggagat
      661 cgccgcgcac cgcgccgaca tcgacggcct cgtcctcggc ctcgcctccg accagacccg
      721 ctatgccggc atcgccaagc ggtagcaccg ctctgcgagc gatcgaaatt cccctctccc
      781 ctcgcgggag agggaggggc ccgttgcgca gcaacgggag ggtgaggggt ctactcaaag
      841 acagatatga tggcgagcaa ccccctcacc ctcccagcgc tgcgcgctgg gcccctccct
      901 ctcccgcaag gggagaggga aatcgatgcg cgcgatgtcg atgactcgat attttgcaac
      961 gacgctgcgc gtcctggcgc aggtgctgct gacgctgctc ggtcttctgc tcgcgacgtt
     1021 tctcattgcg cgcgtcgtgc cgatcgatcc cgttctcgcg gtcgtcggcg atcgtgcctc
     1081 gagcgagacc tatgcgcgag tgagaagcga gctcgggctc gaccggccgc tttacgtgca
     1141 gttctatcgt tatgtcgcga ccgcttcgcg cggcgatttc gggcgttcgg tgctgacgcg
     1201 caatctcgtc acggacgaca tcgcgcgctt cctgccggcg acgatcgagc tggcgacgct
     1261 ggcgacgctg ctcggcgtcg ggctcggcct tccgctgggc gtcgtcgcgg cggcccgtca
     1321 tggacgctgg cccgatcatc tgatccgctt cgtcgcactc gccggctatt cggtgccggt
     1381 cttctggctg gggctgatgg cgctgctgct cttctacgcc ggactgggct gggccgccgg
     1441 cccgggacgg ctcgacatct atctcgagga cagcatcagg ccggtgaccg gccttctcct
     1501 cgtcgacagc gtcattgccg gcgattgggg ggtgctccgg agcgcgctcg cgcatctcgt
     1561 gctgccggcg agcgtgctgg gatacttcgc gctcgcttac gtcagccgca tgacgcggag
     1621 cctcatgctc gatcagctcg gccaggaata catcgtcacc gcgcgcgcca agggcttgag
     1681 cgagcgccgc gtgctgtggc gacacgcgct cggcaacgtg atgctgccgc tcatcacggt
     1741 ggtggcgctc tcgtatgcga gcttgctcga aggctctgtt ctgaccgaga cggtgttcgc
     1801 ctggcctggc atcggctcct acatcacgag ctcgctgctc gccatcgaca tgaacgcggt
     1861 gctcggcggc acgctggtgg tcggcaccat cttcatcgcc gtcaacctgc tgtcggacct
     1921 cctctatcgc tggctcgatc cgagggcccg atgagggcgg cggcgtggca tgactggctc
     1981 accgccgagg cgcccggctc ggcgcgccag gcgcgcctcg ccgaagctta tcgcctgctg
     2041 cgcgccttcg cgcgccatcg cgccggcatg accggcctcg tcatcgtgct gctgctcgtc
     2101 gccgcggcca tcctcgcgcc actgctggca cgcttctcgc cgtcggcgca ggatctggcg
     2161 ctgcgcctca agccgcccga cgcggtccat tggctcggca ccgacgagct cagccgcgac
     2221 atcttctcgc ggctccttta tggcgcgcgc gtcacgctga gcatcgtcgc gctggtgacg
     2281 gcgatctcgg cgccactcgg cctcgccatc ggctgcaccg ccggctatgc cggcggcatc
     2341 ctcgacgcag gcctgatgcg gctcaccgac gtgttcctgt cgctgccgcg gctcatcctg
     2401 gcgctggcgc tggtggcggc gctgggcccg ggcatcggca atgccgcgct ggcgatggcg
     2461 ctcgtggcct ggccgcccta tgcgcgggtg gcgcgcgccg agaccctggc gctgcgcggc
     2521 agcgacttca tcgccgcggc gcggctcgcc ggagcatcgc ccgcgcgcat cgtgctgcgc
     2581 cacatcatgc cgctctgtct cgcctcggtg atcgtgcgcg tgagcctcga catggcgggc
     2641 gtgattctga tcgccgcggg cctcggcttc ctcggcctcg gcgcgcagcc gccgacgccc
     2701 gaatggggcg cgatgatcgc cgcagggcgc cgctacatgc tcgaccagtg gtgggtcgcg
     2761 gccatgccgg gcctcgccat cgctgtggcg agcctcggct tcaacctgct gggcgacggc
     2821 ttgcgcgacg tgctcgatcc gaagcagcga tgagcgcggc gctgctcgaa atcgcggagc
     2881 tgcggctggg cttcggcggc gaccgtcctt tcgaggcgct atcgggcgtc ggcttcacca
     2941 tgggtcgcga gcgcctcggc atcgtcggcg agtccggctc gggcaaatcg ctcaccggcc
     3001 gctccatcct gcgcctgccg ccgccggatg cggtcatgag cgcgcgccgc ctcagcttcg
     3061 acggtatcga cctgctcacc gccggcgaag acgcattgcg cgcgctgcgc ggccggcgga
     3121 tcggcctcgt gctccaggat ccgcgcttct cgctgaaccc ggtgatggcg gtcggccggc
     3181 agatcgccga ggcctatcgc gcgcatggtc gcgccggtgc gcgcgaggcg cgggagaagg
     3241 cgctcgccat gctggaagcc gtgcgcatcc gcgagccagg gcgcgtcttc gatctctatc
     3301 cgcatcagat ctcgggcggc atggggcagc gcgtgatgat cgcgatgatg ctggcacccg
     3361 gccccgacct gctgatcgcc gacgagccga cctcggcgct cgacgtcacc gtggccatcg
     3421 acgtgctgtc gatcctcgac gaggaggtga gccggcgcgg catgggtctc gtcttcatca
     3481 gccacgatct cgatctcgtc gccggcttct gcgaccgcgt gctggtgatg gaacgcggcc
     3541 ggatcgtcga gagctgcgaa gcggccgcgc tcgaatccgc tgagcatccc tatacgcgcc
     3601 gcctgctcgc gtcacggccg cgcctgcgcg cgtcatgatc gaggcgagcg ctctcacggc
     3661 gcgtttcggc gcggtcacgg ccgtcgacca ggtttctttc accgtcgccg gcggcgaagc
     3721 gttcggcatc gtcggggagt cgggctcggg aaagtcgacg ctgctgcgcg tcctcgccgg
     3781 gcttcatgag gattggtccg gtcgactggc cgtcggcggc gccgcgcgcg tggcaaagcg
     3841 aggggctgcg atgagcggcg cggtgcagat ggtcttccag gacccctatg gctcgctcca
     3901 tccgcgccat agcgtcgatc gcacgctcgg cgagccgctt gccattcatc gcatggacgg
     3961 cgctgcggcg cggatcgcgg cggcgctcga cgccgtcggg ctcgagcgcg cgcaacgctt
     4021 ccgctatccg catcagctgt cgggcggcca gcgccagcgc gtggcgatcg cgcgcgcgct
     4081 cattctcgaa ccgaagctcg tgctgctgga cgagccgact tcggcgctcg atgtcacggt
     4141 gcaggcggag atcctgtcgc tgctgcggcg gctgcgacgg gagcgcggac tcacttatgt
     4201 gctggtgagc cacaacctcg ccatcgtggc cgagctctgc gaccgcgtcg ccgtcatgcg
     4261 cgacggccgc ttcgtcgagg agctgagcgc tgccgcgctc gccgccggcg aagcgcggca
     4321 tccctacacg caagagctgc tacgcgcgag ccgcggctat cggcgtcaga ggtgaccgtc
     4381 atacccggcc atgccagcgc tgcggatcgc gccgggcgtg aaagcatgcg atgacaaaga
     4441 cagtctcatc gacgacgcga aaaaacaaag cataagggaa tcggcgaagc ttcgctcggc
     4501 gcacgtcttt cagcacgagg gggaactgca gcgggttatc ggcgatgcgc gtgacgacag
     4561 cgtcgacctc gccaacaaaa cgccgagcca agacttccgc atgcgtagcg taccaatggg
     4621 atgcttcgag catctcagcc cgggcgccag ggccgaagac gacgggaagc actacggtcg
     4681 tcgggtcggc agctcggcct taagctgctc ccatggcacg ccctgggcac gatccgcctc
     4741 aaagcgcgcc aagcggtgct cgagttcggc ctcctgcgcg ggcgtcaaag gcacgtccgc
     4801 gtcggtgagg ctgtcccaga gctcgctgat cagagcgagc cgctgctgcg gcgacagtcg
     4861 ggcgatatcg tcgagcgggc gatctttcat gggtcgagta tggagccggg tcggctaaaa
     4921 ttcaatttag ccgaagccgt tcgcgccctc gtgcccggcg gccaagccgt ggatgccgcc
     4981 cggcgccgcc gtggcagcaa aacagcgcca cggtttggtg gtgccgccga ggctcgtcaa
     5041 gcagcagtgg atcggcatca gttcgccggc gatcgtctcg gcgaccctca accaggtttc
     5101 agatgaccgg cctttgtcag gtagtctcgt tgattgaccg tcaggaaggt cgcatatttt
     5161 gcgactccgc cggaggggaa gggagcattc tcgagcggcg ggcaattccg ggtcttcgca
     5221 tcggcgtccg ctacccaaag gtcataatcg ggcattgtct cgtagatctt gtttttgtac
     5281 tccatccaca tgtgctccgg ggcgttctgg cctctgcgaa acccgccata gacgatgatc
     5341 tgttcgccgg tggtctccga gacgcgtgcc acgccgacga gcgtcttgag agcctcattc
     5401 ctgcttgcct cgttgttggc aactttcagc ctgtttgctg agttgttgta cctgctgttc
     5461 acagacttgg cggtcgtctc gtccttgtat ccatcgattg cccatacatg acaggcgata
     5521 tttttgaagc catattcctt ttcggacgtg tcatctttct tcttcttctc ctcaccgcca
     5581 tcgacgaaag acatgatcaa tacccctccc tatcgggccg catagacctt catttcaggt
     5641 ccggcaaaga catttacgca gcttccgccc tcgcagtcgc cgggcgcgaa tgaccgagcc
     5701 gccggatgat actccgatgt tccggcacct caagcggtcg gtgccgccag tcgagcatgt
     5761 ctgggataac cctgtgatcg gcgtcacgcc ggacgcgcgg tccgccagcg gcggcgcagc
     5821 gtcgcgcggt tggcggcgag ccagtggaga gcgaggaggg tgaaggcgtt gtcgatcttc
     5881 tgtgcccgca tgagccgcat cgcgtcgcga tagctcttca ccaccacctt gatgtcctca
     5941 tgctccgcgg cgacgccgtg gatgccgccg gcgttgcgcg agtcgatgcg cccgcagaac
     6001 aaggcgacgg tctcggtggt gccgcccggg ctcgtcaggc agcgatggat cggcaccagc
     6061 tcgccctgga tggcgagccc cgcttcctcc tgcgcctcgc gccgggcgac gtcgggctcg
     6121 gcctcgccgt cgcgatcgac gatgccggcg acgacttcga cctgccaggc ggggaatccg
     6181 gcaagatgcg ccgcgaggcg gaactgctcc acgagaacga cggcatcgcg ctcgggatcg
     6241 tagaggagga cggcgacggc agcgccgcgc tcgaacactt cgcgctcaag ctccgggctc
     6301 cagccgccgg cgtggaggcg atggcgcacg cgatagcgtt cgatccggaa gaagccctga
     6361 taggcgatct cgcggcagag gatctgcacc gccttgtcgt cgtcgacggg ggaggcggcg
     6421 gaagaagaag cggccgtgct tggcacggcc gcactctagt gggaaagggt cgcgcgcgac
     6481 aaccgggcgc gctcaaccgc aggttcaggt accgctgcgc tgcacgcggt ccgcgacagt
     6541 ggaaacattg cgcgccgtct cggcttcgag ctgctcagcg aggcgcacgc gcatcgccgc
     6601 cacgcatatc gcgagcggcg cagacgcggc gggcgcggat gacggatagg ggatcatcgc
     6661 atcgcgcgca cggtagattt cgccgatcca gccgccgaag ccgccctgac aattgctcat
     6721 ctggccgagg cggtgaatcg tccagccctc gggaaccaag gtcaacgccg cgtcgagcga
     6781 gcgcgtgtaa agcggtacgg gcttcttcgt gccgaagagg cgctgaatgg cgtagtcaag
     6841 atactcgctt ccgtcctggg cgctttgaag cgtcgacaca agcttcgcaa gttccatggt
     6901 gaagccacct tttttgcgtt gcgggagaag ccaaacgcta gcggatcatc gctcgaaagg
     6961 cggaggatca aaatagtgtc aggttcgcgc tttggttggg attgatacga ggcgcgcaac
     7021 ttcggcgcgg gacaagcgca cgcgcatcaa cagcccgttg ggccggcggt tctcggcggt
     7081 aatcgttccg ccgagcgctt cgacgttgcg ccgcgcgatc cacagtccga tgccgaaatg
     7141 cgggccctgg ctctcgccgt tcggcggcgg tgtgcgctgc gagaaatagc gatcgaagat
     7201 gcgcgacaga ttctccggcg gcacgccggg gccgctgtcg ccgatcagga gctcggctgc
     7261 gccgccgacg gtctcgagcc ggacgccgat gctctcgcct tcgggcgaga aggagacggc
     7321 gttgtcgagc agattctcga tcaccgtctc gaccatctcc gcattggcat gcaccagcac
     7381 atgcggcgcg atgtgcccct tgagccggag gcgccgctcg acg
//
LOCUS       DASYNS010000482        10554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_279495, whole genome shotgun sequence.
ACCESSION   DASYNS010000482 DASYNS010000000
VERSION     DASYNS010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279495"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1688)
                     /locus_tag="VGP50_14990"
     CDS             complement(<1..1688)
                     /locus_tag="VGP50_14990"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEV8028738.1"
                     /translation="MSNPMTALAARLRRFIARREGNVAIIFGLAAIPVVVAAGMAVDV
                     TRAYMVKERLSASLDAASLAVGSEGNLTAAQLQTELTNYFVANYPSTALGYNITITPL
                     DSTSDPNGNLAATVVNYQAQATVDMSFMSLVGVPSITVTALAQTHRGYGLEVALVLDN
                     TGSMLCGPNDGAPNYTDGSCAGGVLTSDTSCTNTSNQSRICTLRNASLQFLTTMEGAI
                     TTSGQLYIGVVPYVTTVNVGPTFCTATFTCNNMAQDSCSGDFTDDGGDIVAFPNGVMG
                     TLTSGSKNVTTLNNSTGDTNSPSNTGDASVGMLLRAVNPDSSQVTGPTPIASGTTITS
                     TTGAPPTALALSANAATSGAFVLQVGFTGKVTAGSTTVTNITPNLSTWTNVIPVREVV
                     TDNPNDTSTTPAYTVAGPAIPEDTAVKTVNSAAAVNTITLCNAATSSGTVTFWSYDPI
                     PYDPNTNTPLGNGTDQSAESWMGCVVEPTSSDENSAVAGILNSGVSDPDVSEPSGGWP
                     NWYPFWWRSGQGGFDGGINNWVPKAGGILGQTNTTEVQGDVVTDWDKFSGPNQG"
     gene            complement(1720..2319)
                     /locus_tag="VGP50_14995"
     CDS             complement(1720..2319)
                     /locus_tag="VGP50_14995"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEV8028739.1"
                     /translation="MQKSFHPAARRGARGLIGDRRGTAAIEFAFIFPVMLALFFGSFE
                     ITNLLMLNLKLTAATEDASDLLAQTRSSQNNVAPSDLNNYWNAATMELSPYAAAPYTS
                     AGLQLAFASITFNAGTGAAQILWHNEQNGATPINTAALPQSVLKPLNNGSGTDSVIMV
                     QSQITYSSPLSWVLGKTYTLADIAYNRPRYVLQITCTTC"
     gene            complement(2319..2858)
                     /locus_tag="VGP50_15000"
     CDS             complement(2319..2858)
                     /locus_tag="VGP50_15000"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEV8028740.1"
                     /translation="MRKLLGRLIRERRGAVAIEMAFVGPPFLLTLLSIVELGLILTTQ
                     ALLDGATRDAARLIRTGQVSTAGNTISTFQTLICNDMSMLLTTATCTNNVLIDVVSSP
                     TATNFAGLTFAACASNAGSGGPNPCPFSAGNPGDIVAVQVSYQRPFIIPWVGNLLSVA
                     SDSQHVLLQSNVAFRNEPF"
     gene            complement(3020..4066)
                     /gene="epmA"
                     /locus_tag="VGP50_15005"
     CDS             complement(3020..4066)
                     /gene="epmA"
                     /locus_tag="VGP50_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEV8028741.1"
                     /translation="MSATSEGWWRPERFAAKRKHLEMRARILDAIRRFFAERDFVEVE
                     TPALQRSPGLEPHLMAFATELNEPGAGPRPMFLHTSPEFAMKKLLVAGVPRLFQLARC
                     FRNGESGRAHHPEFTMLEWYRAGAGYRDLMSDCEALLRACLDAAGAAHFTWQGSTADP
                     ARPWTYISVAEAFARFCGIDLLATAPDPLEPRIERLAKAAQPLGIAPHDGDSWEDLFF
                     RIFLERIEPELGSGAPAILYDYPISMAALARAKPGDPHLAERFELYVGGLELANAFGE
                     LTDAGAQRRRFRADQAKKQARYGVTYPIDEDFLAALAHGMPESAGIALGLDRLVMLAS
                     GAPHIEDVLWAPVA"
     gene            4381..4794
                     /locus_tag="VGP50_15010"
     CDS             4381..4794
                     /locus_tag="VGP50_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028742.1"
                     /translation="MKNGLALSTVLGLAMLAACAQSPPPPGQKLAAAELAKVEFNATA
                     IGSDSYENFAIYTAPDGTFAIKMGSVDDTGTYKITDDGHVCLKYHKAFGGNEYCNTIY
                     RDGQTYRAVAPNGAIVGTYTMTPGNPRNLQTAGAR"
     gene            5013..5579
                     /gene="efp"
                     /locus_tag="VGP50_15015"
     CDS             5013..5579
                     /gene="efp"
                     /locus_tag="VGP50_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV8028743.1"
                     /translation="MKINAIDIKPGNVLEHQGKLWLVLRRELVQPGKGGSFAQVEMRD
                     LRGGSKLNERFRTQETVERVRLDEKEMQFLYFDGDHATFMDNETYEQLTLDREMIGEP
                     ADFLHEGMLCQIQLYEGAPLAIELPQTVTMTVTEADPVVKGQTASSSYKTGKLENGRR
                     IMLPPHIAAGTKVVVNTSDGSYVERAKD"
     gene            5590..6402
                     /locus_tag="VGP50_15020"
     CDS             5590..6402
                     /locus_tag="VGP50_15020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV8028744.1"
                     /translation="MAVRSATINVMTAAALKAARGLIRDFGEVEQLQVSVKGPGDFVT
                     TADLRAERLLRTELAKARPGYAMLMEEGGAEAGSDPRHRWIVDPLDGTTNFLHGIPHF
                     AISIALERDGEIIAGVIYEPLRDEMFWAEKGAGAFLNDRRLRVSGRRRLGEAVIGTGI
                     PFAERGDHARYLETLSAVMGATSGVRRMGAAALDLAYVAAGRLDGFWEFGLSPWDIAA
                     GILLIRESGGYVTDLAGGHRMMETGDVLAANDHLHLPLAQLLKKSFGQPAAR"
     gene            6699..6917
                     /locus_tag="VGP50_15025"
     CDS             6699..6917
                     /locus_tag="VGP50_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028745.1"
                     /translation="MSKFDDLSTARRKANELMTKKEAERISSRDKERQVEDAKIARLR
                     GLRLAKEADDKAAAQVAKRVPPARRKPA"
     gene            6934..7104
                     /locus_tag="VGP50_15030"
     CDS             6934..7104
                     /locus_tag="VGP50_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028746.1"
                     /translation="MPFKPKYNLRRSDRQKAQQQKHEEKQLRRQEKAAQRKAERDDEA
                     PSAEDDSGKILS"
     gene            7228..8148
                     /locus_tag="VGP50_15035"
     CDS             7228..8148
                     /locus_tag="VGP50_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl acyltransferase"
                     /protein_id="HEV8028747.1"
                     /translation="MSVEPPIDRFRHRVLRAARRFAHLAEAALLLLVMAVFAALPVDV
                     ASASGGWIARMIGPRLGGFSRRALANLARAFPANDALENRRILRGMWDNLGRAVAEHP
                     HLARICRADSGRVEIVNGGVVDEILAGTRPALLFGGHFANWEIGPSTIHRLMGGTLLS
                     VYRAANNPWVDRMQRWRLPARRAVPKGAEGGRVLLRHLRAGGHVAMLVDQKQNDGIAV
                     PFFGAAAMTAPALGRLGRRFACPILPIRVERLAGAHFRFTVQPPIEIPDTGDAVADVL
                     SVMTRVNAVIEEWVRARPEQWIWLHRRWPG"
     gene            8389..8814
                     /locus_tag="VGP50_15040"
     CDS             8389..8814
                     /locus_tag="VGP50_15040"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEV8028748.1"
                     /translation="MPSIARRYLAYGSNMSRVQMAERCPAARPLGVAVLPGRRFLINR
                     DGYATLVAAPAGCAYGLVWTLDAADESSLDRYENVAAGEYRKETVLLAEFGEALIYIA
                     ADATPGRPVPGYLEGILAAAAAAGLPLAYQAELARWRAA"
     gene            8928..10097
                     /locus_tag="VGP50_15045"
     CDS             8928..10097
                     /locus_tag="VGP50_15045"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV8028749.1"
                     /translation="MTDLINWILEEGLDSDDLGAILGGLCERLVAEGIPLRRVTLGMP
                     TIDPTAATLNFLWWRDKGVTSGALTPEAASGAAFRRSAIRDLQERRVFAERWKLEDPE
                     VARRFVLFEELRAMGMTEYALRLVPFSRRRTALQGAALSVATDRPGGFSDAEIGALVP
                     LLPALGVTAYRIGLLRVATETLGAYLGPKTAAQVLQGKIRRGDSQVISAALLLADLRG
                     FTTLVDRAPGTEVVGWLNQHLECIGDAVTAHGGEILKFLGDGLLAVFAAEPIGAEQAC
                     RDALSAAIEAGERNVGLNVRRSAAGGPTLDLTVALHFGDVVYGNIGTARRLDFTVIGP
                     AVNEVSRMEALGKALGRPLLLSESIARHCGRPVLNLGPHALRGIAGTREMYVVAP"
     gene            10165..>10554
                     /locus_tag="VGP50_15050"
     CDS             10165..>10554
                     /locus_tag="VGP50_15050"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HEV8028750.1"
                     /translation="MARVAPAAATLAALTILSSPAGAASVDCATAASPYDQLICATPD
                     LSAVDEQMSAAYERTRARLSAEGAELLAEGQRSWRQYLRAACPLSADCLRNAYADRFG
                     RLGAAVVTIGPYRFQSVDSYKATQPTPD"
BASE COUNT         1681 a         3486 c         3619 g         1768 t
ORIGIN      
        1 cagccctggt tcgggccgct gaacttgtcc cagtccgtga ccacgtcgcc ttgcacctcg
       61 gtcgtgttcg tctggccaag gatgccgccg gccttgggga cccagttgtt gatgccgccg
      121 tcgaagccgc cttggcccga ccgccaccag aacggatacc agttcggcca accgccggag
      181 ggctcgctga cgtcgggatc gctgacgccc gaattgagta tgcccgcaac ggcgctgttc
      241 tcgtcgctgc tcgtcggctc gacgacgcaa cccatccagc tctcggcgga ctggtcggtg
      301 ccgttgccga ggggtgtgtt ggtgttcggg tcgtagggga tcgggtcata gctccagaac
      361 gtgaccgtgc ccgaggacgt cgccgcgttg cagagcgtga tcgtgttgac ggcagcggcg
      421 gagttcactg tcttgacggc agtgtcctcg ggaattgcgg ggccggcgac ggtataggcg
      481 ggcgtggtcg acgtgtcgtt cggattgtcg gtgaccacct cgcgaaccgg gatcacattg
      541 gtccaggtcg agaggttcgg cgtgatgttg gtaacggtgg tgctcccggc ggtcactttg
      601 cccgtgaagc cgacctgcag gacgaaggcg ccggacgttg ccgcattcgc cgacagcgcc
      661 agcgccgtcg gcggcgctcc cgtcgtcgac gtgatggtgg tgccgctcgc gatcggcgtc
      721 gggccggtga cctgagagga gtcggggttg acggctctga gcagcatacc gacggacgca
      781 tcgccggtat tgctcgggct gttggtgtcg ccggtactgt tgttgagggt cgtgacgttc
      841 ttgctgccgc tcgtcagcgt gcccatgacg ccgttgggaa aggcgacgat gtcgccaccg
      901 tcgtcagtga agtcgccgct acagctgtcc tgagccatgt tgttgcaagt gaacgtggcg
      961 gtgcagaagg tcgggccgac attgacggtc gtcacatagg gaacgacgcc gatatagagc
     1021 tgcccgctcg tcgtgatcgc gccctccatc gtcgtgagga actggagcga ggcgttgcgc
     1081 agcgtgcaga tgcggctctg gttgctggtg ttggtgcagc tcgtgtcgga cgtgaggacg
     1141 ccgcccgcgc aggatccgtc ggtatagttg ggcgcgccgt cattcggacc gcacagcatc
     1201 gagccggtgt tgtcgaggac gagggcgact tcgagcccgt agccgcgatg ggtctgggcg
     1261 agcgccgtca cggtgatgct cggcacgccg accagcgaca tgaaggacat atcgaccgtg
     1321 gcctgggcct ggtagttgac gaccgtcgcc gccaggttgc cgttggggtc gctcgtgctg
     1381 tcgagcggcg tgatggtgat attgtagccg agcgccgtcg agggatagtt ggcgacgaag
     1441 taattggtga gctccgtctg gagctgagcc gcggtgaggt tgccctcgga gcccaccgcc
     1501 agcgaagccg cgtcaagcga ggcgctcagg cgctccttca ccatgtaggc gcgcgtcacg
     1561 tcgacggcca tgccggcggc gaccaccacg gggatcgcgg ccagcccgaa gatgatggcg
     1621 acattgccct cgcgccgcgc gatgaagcgg cgcagccggg cggcgagtgc ggtcatcgga
     1681 ttggacatga cgaaggttcc cttttgatcg cagtgccgct cagcaggtcg tgcaggtgat
     1741 ctgcaggacg tagcgcgggc ggttgtaggc gatgtcggcg agcgtgtagg tcttgcccag
     1801 gacccagctc agcggcgagc tgtaggtgat ctgcgactgc accatgatca cgctgtcggt
     1861 gccgctgccg ttgttgagcg gcttcagcac gctctgcggg agtgcggccg tgttgatcgg
     1921 cgtcgcgccg ttctgctcgt tgtgccagag gatctgcgcg gcgcccgtcc ccgcgttgaa
     1981 ggtgatgctg gcgaaggcga gctgcaggcc ggcgctggtg tagggcgccg ccgcgtaggg
     2041 cgacaactcc atcgtcgccg cgttccaata attgttgaga tcgctgggcg cgacgttgtt
     2101 ctgactggac cgggtctgcg ccaggaggtc ggacgcgtcc tccgtcgccg ccgtcagctt
     2161 cagattgagc atcaggaggt tggtgatctc gaaggagccg aagaacagcg ccagcatcac
     2221 cgggaagatg aacgcgaact cgatcgccgc cgtgccgcgg cggtcgccga tcaggccgcg
     2281 cgctcctcgg cgagccgccg gatgaaatga cttttgcatc agaagggctc gtttctgaag
     2341 gcgacgttcg attgcagcag cacatgctgg ctgtcgctcg ccacgctcag caggttgccg
     2401 acccagggga tgatgaaggg gcgctgatag gacacctgga cggcgacgat gtcgccggga
     2461 ttgccggccg agaacgggca cgggttggga ccgccggacc ctgcgttgga ggcgcaggcg
     2521 gcgaaggtca gccccgcaaa gttggtggcg gtgggactgc tgacgacatc gatgagtaca
     2581 ttgttcgtgc aggtcgcggt ggtcagcagc atcgacatgt cgttgcagat gagcgtctgg
     2641 aacgtgctga tcgtgttgcc ggcggtgctg acttggccgg tgcggatgag ccgcgccgca
     2701 tcgcgcgtcg cgccgtcgag cagcgcctgg gtggtcagga tgaggccgag ctcgacgatc
     2761 gagagcaggg tcagcaggaa gggcgggccg acgaacgcca tctcgatggc gaccgcgcca
     2821 cgtctttccc ggatcagtcg ccccagcagc ttgcgcatgt atttctctct gattttttag
     2881 caaaatccag atctgtctcg gacttgttcg agcgtcatgg acaggagtag agcgtccgtg
     2941 cttgacgaac acttacgaga tcgcccgccg cggcggccta ggcccgctga catagtccgc
     3001 gcgagaattt cgagttatgt cacgcgaccg gtgcccagag cacatcctcg atgtgcgggg
     3061 cgccgctcgc cagcatgacg aggcgatcga gtccgagcgc gatgccggcg ctttcgggca
     3121 tgccatgggc gagcgccgcc aggaaatctt cgtcgatcgg ataggtcacg ccatagcgtg
     3181 cctgcttctt cgcctggtcc gcgcggaagc gccggcgttg cgcgccggca tcggtgagct
     3241 cgccgaacgc attggcgagc tcgaggccgc cgacatagag ctcgaaccgc tcggcaagat
     3301 gcggatcgcc gggcttcgcc cgcgcgagcg ccgccatgga gatcggatag tcgtaaagaa
     3361 tcgccggcgc gccgctgccg agctccggct cgatgcgctc gaggaagatg cggaagaaca
     3421 gatcctccca gctatcgccg tcatgcggcg cgatgccgag cggctgcgcc gccttcgcca
     3481 gcctctcgat tctcggctcg agcggatcgg gcgcggtggc gagaaggtcg atcccgcaga
     3541 accgcgcgaa ggcttctgcc acacttatat atgtccaggg ccgcgccgga tcggcggtgc
     3601 tgccttgcca ggtgaaatgc gcggcgccgg ccgcgtcgag gcaggcgcgg agcagcgcct
     3661 cgcaatcgct catgagatcg cgatagccgg cgcccgcgcg gtaccattcg agcatggtga
     3721 attccggatg gtgcgcgcgc ccgctttcgc cgttcctgaa gcagcgggcg agctggaaga
     3781 ggcgcggcac gcccgctacc agcagcttct tcatggcgaa ttcgggcgag gtatggagga
     3841 acatcggccg cggtccggcg ccgggctcat tgagctcggt ggcgaacgcc atgagatgcg
     3901 gctcgagtcc ggggcttcgc tgcaacgccg gcgtttccac ttcgacgaag tcgcgctcgg
     3961 cgaaaaagcg gcggatcgcg tcgaggatcc gcgcgcgcat ctcgagatgc ttgcgcttcg
     4021 ccgcaaaccg ctccggccgc caccatccct cgctcgtcgc gctcatgccc gctcgtcacg
     4081 ccagcgcaga gggttcttcc gccgcagaat gggcggcagt ccttgcgccg tcatcgtgca
     4141 gcatccatgg ccgtccggtc acttcgcctc gttgagcccg aaggttaccg cgcgcaaacg
     4201 ctcgacagca agccgaagcc ccggcgcggc ggcgggcggc aacgcggcga tgacgcggcg
     4261 aacgcgcccg cgacgcgcag cgattgcatc agcgcggcgc ctgttcgccg ttgccgcccc
     4321 gcgcaacgag gcgtagggtg ataagattgt gcgcggccga tttcacctcg ggaggctgcc
     4381 atgaaaaacg gcttggcgtt gagcacagtg ctcggtcttg caatgcttgc ggcctgtgca
     4441 cagtcgccgc cgccgcccgg ccagaagttg gccgccgccg aactcgccaa agtcgagttc
     4501 aacgcgacgg cgatcggctc ggatagctat gagaacttcg cgatctacac ggcgcccgac
     4561 gggacgttcg ccataaaaat ggggtccgtc gacgataccg gcacctacaa gatcaccgac
     4621 gatggtcatg tttgcttgaa ataccacaaa gcattcggcg gcaacgaata ctgcaacacg
     4681 atctaccgcg acgggcagac gtaccgcgcg gttgcgccca acggcgccat cgtcggcacc
     4741 tacacgatga cgcccggcaa cccgcgcaac ctgcagacgg ccggcgcgcg atagagagtc
     4801 gctccagcga tcgtgaggcg ttggcgcgcc tcatgcgctc gtgttcttga tggcggcgat
     4861 ggcgatcggc ggacggccgt tttgttgatt tgcatatcgt tcgcctgggc ggccgtccag
     4921 ggctgtcccc ttcctttgcc gggcccaagc tgctagtaac ccaccaaatc cttccttcgg
     4981 gagcggccag agcgccagcg aggacgagcg ccatgaagat caatgccatc gacatcaagc
     5041 ccggcaatgt gctggagcat cagggcaagc tgtggctggt gctgcgccgc gaactcgtcc
     5101 agcccggcaa gggcggctcc ttcgcgcaag tcgagatgcg cgacctgcgc ggcggctcga
     5161 agctcaacga gcgcttccgc actcaggaga ccgtggagcg cgtccgcctc gatgaaaagg
     5221 agatgcagtt cctctacttc gacggcgatc acgccacttt catggacaac gagacctatg
     5281 agcagctcac cctcgatcgc gagatgatcg gcgagcccgc ggatttcctc catgaaggca
     5341 tgctgtgcca gatccagctc tatgaaggcg cgccactcgc catcgagctg ccgcagaccg
     5401 tgaccatgac ggtgaccgag gccgatccgg tggtgaaggg ccagacggcg tcctcctcct
     5461 acaagaccgg caagctcgag aacggccggc gcatcatgct gccgccccat atcgcggccg
     5521 gcaccaaggt ggtcgtcaac accagcgacg gcagctatgt cgagcgcgcg aaggattgac
     5581 gggcactgaa tggcggtccg ctccgcgacc atcaacgtca tgaccgccgc cgcgctcaaa
     5641 gcggcgcgcg gcctcattcg cgatttcggc gaggtcgagc agctccaggt ctcggtgaaa
     5701 ggtccgggcg atttcgtcac caccgccgat ctccgcgccg agcgcctgct gcgaaccgag
     5761 ctcgcgaagg cgcgtccggg ctacgccatg ctcatggagg aaggtggcgc cgaggccggc
     5821 agcgacccgc gtcatcgctg gatcgtcgat ccgctggacg gcaccacgaa tttcctccac
     5881 ggcattccgc atttcgccat ttcgatcgcg ctcgagcgcg acggcgagat catcgccggt
     5941 gtcatttacg agccgctgcg cgacgagatg ttctgggccg agaaaggcgc gggcgccttc
     6001 ctcaacgatc ggcgcctccg cgtctcggga cggcgccgcc tcggcgaagc cgtgatcggc
     6061 accggcattc ccttcgccga gcgcggcgat cacgcgcgct atctggagac gctgagcgcc
     6121 gtcatgggcg ccaccagcgg cgtgcgccgc atgggtgcgg ccgccctcga cctcgcttat
     6181 gtcgcggccg gacggctcga cggcttctgg gaattcggcc tttcgccctg ggacatcgct
     6241 gccggcattc tgttgatccg cgaatccggc ggctacgtca ccgatctcgc cggcggccat
     6301 cgcatgatgg aaaccggcga cgtcctcgcc gccaacgatc acctccatct gccgctggcc
     6361 cagctcctca agaaatcctt cggccagccc gccgcgcggt gacgaaaacc cctctcccgc
     6421 atcccgcggg cgtgccgtgc ctgtgccaac cccgcgtatt ttaaggcgtg gttaacggaa
     6481 cctgcgtcat cgccgcctgt gatgctcact tgccttttgg ctggcgcggg gtcatgatcg
     6541 gaaacaggct gtgcgccggc gctcgccgta tcgccctccg ggcgtttcta caacatccac
     6601 agaggtcgac acctggttgc tccgcgccgc gctcggtgtg cgcagcaatg tgcgcagaaa
     6661 gaagaggccg ctctgcggag agcccagaag gagcagcgat gagcaagttc gacgatctga
     6721 gcaccgcgcg ccgcaaggcg aatgagctca tgaccaagaa ggaggccgag cgcatctcct
     6781 cccgcgacaa ggagcggcag gtcgaggacg cgaagatcgc gcggttgcgc gggctgcgtc
     6841 tcgccaagga agcggacgac aaagcggccg cccaggtggc gaagcgggtg cccccggcgc
     6901 ggcgcaagcc ggcctgaggc gccgggacac gcgatgcctt tcaagcccaa atacaatctg
     6961 cggcgctccg accgtcagaa ggcgcaacag cagaagcacg aggaaaagca actgcgccgc
     7021 caggaaaagg ccgcgcaacg caaggcggaa cgcgacgacg aggcgccgtc tgccgaggac
     7081 gattcgggca agattttgag ttaggcagac ctcggtcgcg caagaccggg ggaacaaaat
     7141 cgctcggccg atccggcgcc cggtgcgggg cgcgcgggat gtgggtgact gcgcacccat
     7201 cgatgtgccg acagaaccgg acgtaggttg agcgtcgaac cgccgatcga tcgtttccgc
     7261 catcgcgtcc tgcgcgccgc gcgccgcttc gcgcatttgg ccgaagcggc gctgctgctg
     7321 ctcgtgatgg cggtcttcgc cgcgctgccg gtcgatgtcg cctcggcctc gggcggctgg
     7381 atcgcccgca tgatcggccc gcgactcggt ggcttctcgc gccgcgcgct cgccaatctc
     7441 gcgcgcgcgt tccccgcgaa cgacgcgctc gagaaccggc gcattcttcg cggcatgtgg
     7501 gacaatctcg ggcgcgccgt tgccgagcat ccgcatctcg cgcgcatctg ccgtgccgat
     7561 tccgggcggg tcgagatcgt caatggcggg gtcgtcgacg agatcctggc cggcacccgc
     7621 ccggcgctgc tcttcggcgg gcatttcgcg aattgggaaa tcgggccgtc gacgattcat
     7681 cgtctgatgg gcggcacgct cctctcggtc tatcgcgccg ccaacaatcc ctgggtcgac
     7741 cgcatgcagc gctggcgcct ccccgcgcgg cgcgccgtcc ccaaaggcgc cgagggcggg
     7801 cgcgtgctgc tgcgccatct ccgcgccggc ggccatgtcg cgatgctcgt cgaccagaag
     7861 cagaatgacg gcatcgccgt gcctttcttc ggcgcggcgg cgatgacggc gccggcgttg
     7921 gggcggctcg gccggcgctt cgcttgcccc atcctgccga tccgtgtcga gcgcctcgcg
     7981 ggcgcgcatt tccgctttac cgtgcagccg ccgatcgaga tccccgacac cggcgatgcc
     8041 gttgccgacg tgctgagcgt gatgacgcgc gtcaacgccg tgatcgaaga gtgggtccgg
     8101 gcgcgtcccg agcaatggat ctggctgcat cgccgctggc ccggatgagc gcgcgcgtga
     8161 gcggacattt cgcgtctagc caagcgccaa acatcccctc tcccgcatcg cgggagaggg
     8221 agggacccac tgcgaagctt cagcgaagca gtgggagggt gaggggatct ctcaggtaaa
     8281 gtgcgtttgc ttgaagcgcc cgcgagagtg gcgggcggcc ggcacttgcg ccagactgcc
     8341 gtggcaaatc gcgattgtcc ccgaccgcag ccgagacgag atcgatccgt gccgtcgatt
     8401 gcccgccgct acctcgctta cggctccaat atgagccgcg tgcagatggc cgagcgctgt
     8461 ccggcggccc ggccgctcgg cgtggcggta ttgccgggcc ggcgcttcct gatcaaccga
     8521 gacggctatg ccacgctggt tgccgcgccc gccggatgcg cctatggctt ggtctggacg
     8581 ctcgacgcgg cggatgaatc cagcctcgac cgctacgaga acgtcgcggc gggcgagtac
     8641 cgcaaggaga cggtgctcct cgcggagttc ggcgaggcgc tgatctatat cgccgccgat
     8701 gcgacaccgg gacggccggt tccgggttac ctcgaaggca ttcttgccgc cgctgccgcc
     8761 gccggcctgc cgctcgccta tcaggccgaa ttggcgcgct ggcgcgcggc gtgagggccc
     8821 ggactcgccg atcgcgaaag gacgccgtcg acccgctgtt tcccggcgcg gccttgccgg
     8881 gcgtcatgcc agcggataaa gcacgggctc gggccggggg ccgccgcatg accgatctga
     8941 tcaattggat acttgaagag ggcctcgaca gcgacgacct gggcgcgatc ctcggcgggc
     9001 tctgcgaaag gctggtcgcc gagggaattc cgcttcgccg cgtcaccctg ggcatgccga
     9061 ccatcgatcc caccgcggcg acgctgaatt tcctgtggtg gcgcgacaag ggtgtgacga
     9121 gcggcgcgct gacacccgag gctgcctccg gcgccgcgtt ccggcgcagt gcgatacggg
     9181 accttcaaga gcggcgcgtc ttcgccgagc gctggaagct cgaggatccc gaggtggcgc
     9241 ggcgcttcgt tctgttcgaa gagctgcggg ccatgggcat gaccgaatac gccctgcgcc
     9301 tggtgccgtt cagccggcgt cgcaccgccc ttcagggcgc agcgctctcg gtcgcgaccg
     9361 accgccccgg cggcttcagc gacgccgaaa tcggcgccct cgtcccgctc ctccccgcgc
     9421 tcggcgtcac cgcctatcgc attggcctgt tgcgcgtcgc gaccgagacg ctcggcgcgt
     9481 atctcggtcc gaagaccgcg gcgcaggtgc tccaaggcaa gatccggcgc ggcgacagcc
     9541 aggtcatttc ggcggcattg ctgctcgccg atctccgcgg cttcacgacg ctcgtcgacc
     9601 gtgcgccggg caccgaggtc gtcggctggc tcaatcagca tctcgaatgc atcggcgacg
     9661 cggtgacggc gcatggcggc gaaatattga agttcctcgg cgacgggctt ctcgccgtct
     9721 ttgctgcgga gccgatcggc gccgagcagg cgtgccgcga cgcgttgagc gccgccatcg
     9781 aggccggcga gcggaacgta ggcctgaacg tccggcgcag cgccgcgggc gggccaacgc
     9841 tcgacctcac cgtcgccctc catttcggcg atgtcgtcta cggcaacatc ggcacggcgc
     9901 ggcgcctcga tttcacggtc atcggcccgg ccgtcaacga ggtgagccgc atggaggcgc
     9961 tcggcaaggc gcttggacgc ccgctgctgt tgtccgagag catcgcccgg cactgtggcc
    10021 gccccgtgct caatctcggg ccgcacgcgc tgcgcggcat cgccggcacg cgcgagatgt
    10081 acgtcgtcgc gccctgacgg cgactcgatg cgcgctccgg tgtcgacgac cacaggccga
    10141 cgacgacggg accggccgcc gcttgtggca cgcgtcgccc cggcggccgc gacactcgcc
    10201 gcgctaacga tcctgtcgag cccggccggc gccgcgagcg tcgactgcgc gacggcggcg
    10261 tcgccttacg accagctcat ctgcgcgacg cccgacctct cggcggtcga cgagcaaatg
    10321 tccgccgcct atgagcggac ccgcgcccgg ctctccgccg agggcgcgga gctcttggcc
    10381 gaaggccagc ggtcctggcg ccagtacctg cgcgccgcct gcccgctcag cgccgactgt
    10441 ctgcggaatg cctatgccga ccgctttggc cggctcggcg ccgccgtcgt gacaatcggc
    10501 ccttaccgct tccaatccgt cgacagctac aaggccaccc agcccacgcc cgac
//
LOCUS       DASYNS010000483         5680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_900550, whole genome shotgun sequence.
ACCESSION   DASYNS010000483 DASYNS010000000
VERSION     DASYNS010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900550"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VGP50_15055"
     CDS             <1..582
                     /locus_tag="VGP50_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020593420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8028751.1"
                     /translation="KLNIPTDGGAKGVSVVKQGFNVDPLLQKQASCISTMTYNEYWQV
                     IDAGFKPDDLIVFKYEDEGVSTLEDGLYANGDKLKDAAYADKLARFVAASDRGWAWAV
                     KNPDAAAAIVLENDASGAQTEKHQKRMMGEIAKLVDVAKPLGYLNPADYERTVSVLLS
                     SQSSPVITKKPEGAWTQAVYDASKKYWDPASGK"
     gene            660..1361
                     /locus_tag="VGP50_15060"
     CDS             660..1361
                     /locus_tag="VGP50_15060"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEV8028752.1"
                     /translation="MAIVGAAACFAPGASAQPAPPQDSTETRIVVSGDGSVEVAPDTA
                     RLSGGVTAKAKTVKEAVDTTSKTMAAMTAALLQSGIAQKDIQTNRFSVQPVYAAPDPH
                     GDQAVVGYSVSNQVTVKLHDIDKVGPTLDRLVAAGATNVGNVVFEVSDPSTALDKARE
                     AAITDAKRKAELYARAAGIALGGVVWVKEESASAAPMQMRELTVLRGGAAPVPIATGE
                     DTLRARVTVGFGAAH"
     gene            complement(1425..2435)
                     /locus_tag="VGP50_15065"
     CDS             complement(1425..2435)
                     /locus_tag="VGP50_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028753.1"
                     /translation="MFEAQRPFVEMALGFLRLGNTALDAIFYDVNALSADLHRHGETI
                     VTDDEVPAAATSHAPAWPRHNLDLSALANPEGAVLIVDTTQPPYVSKPYLDLAEFVAD
                     ANTHRPAPRNLSITGVGSSALGSVAFAWNISKALGEPVAAIVPGYGLADVVQQALGGW
                     FVFGPQSLLLKMSTQAMMAHMAPEIANIGHRLMMSAPEHAETENGGALFQRGSGSSDV
                     LLGILKRVPSIQRLFGHSKGALVIQNALIDDDESLIGRLEIVTFGCAIATRSPPARLL
                     QFLGMLDWLGWLNSWGNAPTNALETWHSTNSWMPLSMRVADLARADHVYPPAPVSVSS
                     LA"
     gene            complement(2585..2658)
                     /locus_tag="VGP50_15070"
     tRNA            complement(2585..2658)
                     /locus_tag="VGP50_15070"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2624..2626),aa:Gly,seq:ccc)
     gene            complement(2695..3600)
                     /locus_tag="VGP50_15075"
     CDS             complement(2695..3600)
                     /locus_tag="VGP50_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV8028754.1"
                     /translation="MSSRETDSREQRRASGAGATSEVLARLTRDAVLRHSPCGEGTLV
                     WRQWGAGPPLVLLHGGYGAWTHWLRNIDALAARYRVIAPDMPGLGDSAMPPVPYTPES
                     LAAILDRGLDTVLAPGERCHLVGFSFGAALGGHVAAARGAGLLSFTLVGAPSLGLRRG
                     EMRPLEPMRRHMSDAEMAELQRANLGILMIADASRIDDTAVRLQCENVARARVKSRGY
                     ARTDILARVLPLVTAPLGGIWGEYDVTAYPHIGLRAELLRRIQPGAFFEVIAGAGHWV
                     QYEAPEAFNATLLRHLAALEAARGA"
     gene            3764..4156
                     /gene="apaG"
                     /locus_tag="VGP50_15080"
     CDS             3764..4156
                     /gene="apaG"
                     /locus_tag="VGP50_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEV8028755.1"
                     /translation="MYSETTRSIRVTVKPFYLEDQSSPADNHYVWAYHVRIENQGGET
                     VQLRRRHWRITDGLGRVQEVRGPGVVGEQPVLPPGETFEYTSATPLSTPSGIMVGSYE
                     MEAADGTSFAVAVPPFSLDSPHQPIHLN"
     gene            4198..4860
                     /gene="folE"
                     /locus_tag="VGP50_15085"
     CDS             4198..4860
                     /gene="folE"
                     /locus_tag="VGP50_15085"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV8028756.1"
                     /translation="MSDKPPSLRPVNPRPAEAKNGAARHRPGRAEAEAAVRTLIQWAG
                     DDPDREGVKGTPGRVVRAFEDYFSGYDTDPVELLQRTFEETDGYDEMVVLKDIRFESH
                     CEHHLAPIIGKAHVAYLPQKRVIGISKLARLVEVYARRLQIQEKMTAQIANTLDEVLQ
                     PKGVAVVIEAAHQCMTTRGVHKPGVSMVTSRMLGAFRTDASTRREFLAIIGQHGGGSQ
                     YT"
     gene            complement(5021..5479)
                     /locus_tag="VGP50_15090"
     CDS             complement(5021..5479)
                     /locus_tag="VGP50_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028757.1"
                     /translation="MTGGLALRLAVLFLLPLAGCGEGTVPLGEPLERITATFGGLSDV
                     VVVTAVDRLPLRSAALVDPDGQRIPAYSLDVSSSPAVEPSIGEQAMMQTPGMPHEVTH
                     VDTMAATALIRLPDPTRYAKTWQQWHVELQLGDPGGGEQDITLPAPAPPR"
     gene            complement(5481..>5680)
                     /locus_tag="VGP50_15095"
     CDS             complement(5481..>5680)
                     /locus_tag="VGP50_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028758.1"
                     /translation="LEIVDDHGVPLRSVAVKVTRSHTVLEGITPNERDQAWYDMTKML
                     MADFDQQMTAEISAHFGGFFQ"
BASE COUNT         1009 a         1877 c         1954 g          840 t
ORIGIN      
        1 aagctcaaca tcccgaccga tggcggcgcc aagggcgtga gcgtggtgaa gcagggcttc
       61 aacgtcgacc cgctgctgca gaagcaggcg tcctgcatct cgacgatgac ctacaacgag
      121 tattggcagg tgatcgacgc cggcttcaag cccgacgacc tcatcgtgtt caaatacgag
      181 gacgaaggcg tctcgacgct cgaggatggg ctttatgcca atggcgacaa gctcaaagac
      241 gcggcctatg ccgacaagct cgcgcgcttc gttgcggcgt cggaccgcgg ctgggcctgg
      301 gcggtgaaga atccggacgc cgccgcggcg atcgtcctcg agaacgacgc gagcggcgcg
      361 cagaccgaga aacaccagaa gcgcatgatg ggcgagatcg ccaagctggt cgacgtggcg
      421 aagccgctcg gctatctcaa tcccgcagat tacgagcgca cggtcagcgt gctcctgtcg
      481 tcccagtcgt cgccggtcat caccaagaag cccgaaggcg cctggacgca agccgtctac
      541 gacgcctcga agaaatattg ggatccggcg tccggcaagt agcgcgacgc cgcgcagcac
      601 aagggaccat catgccgatc ctcacagcgc gtgacagacg cgtgtcattc gcggcgacga
      661 tggcgatcgt cggagcggct gcctgcttcg cgccgggcgc ttcggcgcag ccggcgccgc
      721 cgcaagacag cacggagacg cgcatcgtcg tcagtggcga tggcagcgtc gaggtcgcgc
      781 cggatacggc acggctgagc ggcggcgtca cggccaaggc gaagaccgtg aaagaggcgg
      841 tggacacgac atcgaagacg atggccgcga tgacggcggc gttgctgcag tcgggcatcg
      901 cgcaaaagga catccagacg aaccgcttct cggtgcagcc cgtctacgcg gcaccggacc
      961 cgcatggcga tcaagcggtg gtgggctaca gcgtgtcgaa ccaggtgacg gtgaagctcc
     1021 atgacatcga caaggtcggt ccgacgctcg accggctggt cgcggcgggt gcgacgaatg
     1081 tcggcaatgt cgtcttcgaa gtgtccgatc catcgacggc gctcgacaag gcccgcgagg
     1141 ccgcgatcac cgatgccaag cgcaaagccg agctctatgc gcgcgctgcg gggatcgcgc
     1201 tcggaggcgt cgtctgggtg aaagaggagt cggcctccgc cgcgccaatg caaatgaggg
     1261 aactcaccgt attgcgcggt ggggcggccc cggtgccgat cgcaacgggc gaggatacgc
     1321 tgcgcgcgcg ggtcacggtc ggattcggcg ccgcgcattg agcgcgcggg cgcggatcgg
     1381 agcagcgtta cagctccgcg tcgcctgcta tcgatgatgc ggcgtcaggc aagcgagctg
     1441 acgcttacgg gcgcgggcgg atacacgtga tcggcgcgcg ccagatccgc cacgcgcatg
     1501 ctgaggggca tccacgaatt cgtgctgtgc catgtctcga gggcgttcgt cggcgcattt
     1561 ccccaggagt tcagccagcc cagccagtcc aacatgccca ggaactgcag caatcgcgcc
     1621 ggcggcgatc gcgtggcgat cgcgcagccg aaggtcacga tctcgagccg gccgatcaag
     1681 ctctcgtcgt cgtcgatgag cgcattctga atgacgagcg cgcctttgct gtggccgaac
     1741 aggcgctgga tactcggcac ccgcttcagg atgccgagca gcacatccga cgagccggag
     1801 ccgcgttgaa acagggcgcc gccgttctcg gtttcggcat gctcgggcgc gctcatcatc
     1861 agccggtgcc cgatattggc gatctcgggc gccatgtggg ccatcatcgc ctgcgtcgac
     1921 atcttgagga gaaggctctg cgggccgaag acgaaccagc cgccgagcgc ctgctgcacg
     1981 acgtcggcga ggccatagcc cggcacgatc gccgcgacgg gttcgccgag cgccttcgaa
     2041 atgttccagg cgaaggcgac ggaaccgagc gcagagctgc cgacgccggt gatggataga
     2101 tttctgggcg ccggccggtg cgtgttggcg tcagcgacaa actctgcgag atcgagatag
     2161 ggcttcgaga catagggcgg ctgcgtcgta tcgacgatca gcaccgcgcc ttccggattg
     2221 gcgagcgcgc tcagatcgag attgtggcgc ggccaagcag gcgcatgcga cgtggccgcg
     2281 gcgggcactt cgtcatcggt gacgatcgtc tcgccgtgac gatggagatc ggccgatagc
     2341 gcgttcacgt cgtagaagat cgcatcgagc gccgtattgc cgaggcgcag gaagccgagc
     2401 gccatttcca cgaatggccg ctgcgcctca aacatattga gagccatgct gcaccttccc
     2461 ggtcggcttc accgttctga tgctcgggcg taacacggcg gagacgtccg ctcgtgtgaa
     2521 gcagctcaca ggcccggcag cggcccagcg tcgcaccacg caacatcttt ggtccgagag
     2581 ggtttggagc gggtgaaggg aatcgaaccc tcgtcgtaag cttgggaagc ttctgctcta
     2641 ccattgagct acacccgcat ccggcgacca tttaagcgga gaggcggctc cgcttcaagc
     2701 gccgcgcgcg gcctccagcg ccgccagatg gcgcagcagc gtcgcgttga acgcctcggg
     2761 cgcctcgtac tgcacccagt ggccggcgcc ggcgatcacc tcgaagaagg cacccggctg
     2821 aatgcggcgc agcagctcgg cgcgcaagcc gatatgggga taggcggtca cgtcatactc
     2881 gccccagatg ccgccgagcg gcgcggtcac cagcggcagc acgcgcgcca ggatatcggt
     2941 gcgcgcgtag ccgcggctct tcacccgggc gcgcgccacg ttctcgcatt gcaggcgcac
     3001 ggccgtgtcg tcgatgcgcg acgcgtcggc gatcatgagg atgccgagat tggcgcgctg
     3061 caactcggcc atttccgcgt cgctcatatg gcggcgcatc ggttcgagcg gccgcatctc
     3121 gccgcgccgg agaccgaggc tcggcgcgcc gaccagcgtg aaggacagca gccccgcgcc
     3181 gcgcgccgcc gcgacatggc cgcccaacgc cgcgccgaag gagaagccga cgagatggca
     3241 gcgctcgccc ggcgcgagca cggtgtcgag gccgcggtcg aggatggcgg cgaggctctc
     3301 gggcgtgtag ggcacgggcg gcatcgcgga gtcgcccagc cccggcatgt ccggcgcgat
     3361 gacgcgataa cgggcggcga gcgcgtcgat gttgcgcagc caatgcgtcc acgcgccgta
     3421 accgccgtgg agcagcacca gaggcgggcc ggcaccccat tgccgccaga ccagcgttcc
     3481 ctccccgcac ggagaatgcc gcagcacggc gtcccgcgtg agccgggcga gaacctcgct
     3541 cgtggcgccg gcgccgctcg cccggcgctg ctcgcgggaa tccgtctcgc gggaagacat
     3601 tgggggaatg ccgcctccgg ccgcttggcg cggccgggca ggcttaccgt caaatccggc
     3661 caaagccaag cctcgccacc gcggagcgcc gcttgacggc tcgcagcccc ggtacagaat
     3721 gggcagagcg gcgcccctga cccgacggag aaagagcccg ctcatgtatt cggaaacgac
     3781 ccgatccatc agggtgacgg tcaagccctt ctacctcgag gaccagtctt cgccggcgga
     3841 caaccactat gtctgggcct atcacgtccg catcgagaat cagggcggcg agaccgtgca
     3901 gctgcgccgc cgccattggc gcatcaccga cggcttgggc cgcgtgcagg aggtgcgcgg
     3961 cccgggcgtg gtgggcgagc agccggtgtt gccgccgggc gagaccttcg agtatacgag
     4021 cgccacgccg ctcagcacgc cgtccggcat catggtcggc agctatgaga tggaggcggc
     4081 ggacggcacc agcttcgccg tcgccgtccc gcccttctcg ctcgacagcc cgcatcagcc
     4141 gatacacctt aattgacgcg cctcgaccgc gtccgtcccc gcaggagcct tagacccatg
     4201 tccgacaaac ccccttctct gcgcccggtg aaccctcgcc cggccgaggc caagaacggg
     4261 gcggcacggc accggccggg acgcgccgag gccgaggcgg cggtgcggac gctgatccag
     4321 tgggccggcg acgaccccga ccgcgagggc gtgaaaggca cgccgggccg cgtcgtgcgc
     4381 gccttcgagg actatttctc aggctacgac acggaccccg tcgagctgct gcagcgcacc
     4441 ttcgaggaga ccgacggcta tgacgagatg gtcgtgctca aggacatccg cttcgagagt
     4501 cattgcgagc atcatctggc gccgatcatc ggcaaggcgc atgtcgccta tctgccgcag
     4561 aagcgcgtca tcggcatctc gaagctggcc cgcctcgtcg aagtctatgc gcggcggctg
     4621 cagatccagg agaagatgac ggcgcagatc gccaacacgc tcgacgaggt gctgcagccc
     4681 aagggcgtcg ccgtagtgat cgaggcggcg catcaatgca tgacgacgcg cggcgtccac
     4741 aagcccggcg tctccatggt gacctcgcgc atgctgggcg cgttccgcac cgacgcgtcg
     4801 acccggcgcg agttcctcgc catcatcggc cagcacggcg gcggcagcca gtacacctga
     4861 gggctgccga gcgcgttcag gttggcagaa cggttcgtcg aagccgcatg ctgctgcaaa
     4921 aaaagatgaa cgcggaggac cggaggcgcc gcggaggacg cagaggaaag tcgttcctgc
     4981 gccctttgcg taacctccgc gtcctctgcg ttcgctaatg tcagcggggc ggcgctggcg
     5041 ccggcaatgt aatgtcttgc tcgccgccgc cggggtcgcc gagctgcagc tcgacatgcc
     5101 attgctgcca agtcttggcg tagcgcgtcg gatcagggag ccggatcagc gccgtcgcgg
     5161 ccatggtgtc gacatgcgtc acctcatgcg gcatacccgg cgtttgcatc atcgcctgct
     5221 ccccgatcga gggttcgacc gccggcgagg agctcacgtc gagcgaatag gcgggaatgc
     5281 gctggccgtc gggatcgacc agcgctgcgc tgcgcaacgg cagccggtcg acggcggtga
     5341 cgacgaccac gtcgctcaac ccgccgaagg tcgcggtgat gcgctcgagc ggctcgccga
     5401 gcggcaccgt gccctcgccg cagccggcga gcggcagcag gaagagcacg gcgaggcgga
     5461 gcgcgagccc gccggtcatg ctattggaag aagccgccga aatgggcgct gatctcggcg
     5521 gtcatctgtt gatcgaaatc cgccatcagc attttcgtca tgtcatacca ggcttgatcg
     5581 cgctcattgg gcgtgatgcc ctcgagcacc gtgtgcgagc gcgtcacttt gacggcgacg
     5641 ctgcgcaagg gcacgccgtg atcgtcgacg atctcgagca
//
LOCUS       DASYNS010000484         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_279726, whole genome shotgun sequence.
ACCESSION   DASYNS010000484 DASYNS010000000
VERSION     DASYNS010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279726"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VGP50_15100"
     CDS             <1..265
                     /locus_tag="VGP50_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028759.1"
                     /translation="SLIIPPSTILPLHEAAPSRHKTVLRYEGEHGVSLRHVGALVGER
                     AHRELWPEILNWAMHLASQEPITGRRRRSPRARAAGGRGVGAV"
     gene            complement(424..1347)
                     /gene="rarD"
                     /locus_tag="VGP50_15105"
     CDS             complement(424..1347)
                     /gene="rarD"
                     /locus_tag="VGP50_15105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEV8028760.1"
                     /translation="MRDGKGDERRAGLLYALGAFGLWGLVLPVYIKALAAVPVAEILA
                     HRVVWAALLALALLALLGRLRVLRTVLTPRSLGLLVLSAALVTINWVTYVVAVTSDHL
                     LAASLGYFLNPLVSIALGLLVLGERLRPRQLWACAIAAFGVLILAAAIRGVPWIALSL
                     ALSFGCYGLVRKVVAAEALAGFTVEVTILAPFAAGYLLWLAATGRGAFALASPGLDML
                     LLGSGLFTALPLVWFAAAARKLPLTTIGLLQFTAPSAAFLLGVFVYGEPFGAAQAACF
                     GCIWLALAVYSLDTVMGARASAIKVPGAASD"
     gene            1484..2041
                     /locus_tag="VGP50_15110"
     CDS             1484..2041
                     /locus_tag="VGP50_15110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8028761.1"
                     /translation="MVNIRQARLADARAIGRIEVETWRATYAGMLSDRVLLSMSTERQ
                     TASWAGFLRHRPGDVFVAQQPAGVVGFGNCGPQREGSFGYGGEVYTLYVTPDAQGTGC
                     GRQLLLALFARLLHCGHRSALIWVLRANPARFFYERLGGKLVLHRAIPVSGEAAEALG
                     YGWSDLRALLDREARSGAGRSSPPP"
BASE COUNT          417 a          866 c          899 g          373 t
ORIGIN      
        1 aagcctcatc attcctccgt cgacgatcct gccgctccat gaagcggcgc ccagccgcca
       61 taaaacggtg ctgcgctacg agggcgagca cggcgtctcg ctgcgtcatg tcggcgctct
      121 cgtcggcgag cgcgcgcacc gtgaactctg gcccgaaatc ctgaactggg cgatgcatct
      181 cgcgagccaa gagcccatta cgggccgccg ccgtcgttcc ccgagagctc gggcggccgg
      241 cgggcgggga gtgggcgcgg tgtgacgcga tgtcgagccg gcgatcatgc cgatgcacag
      301 tcgccccatg ctgtgacgac catgtccgga ttcggcgagc cagcgtgtct gtcggtccct
      361 agtctccgcg tcgggaggta gagaccatgc acgacctgca tggtgttgtc ggcttgatcc
      421 cgatcaatcc gacgccgcgc ccggcacttt gatcgcgctc gctcgcgccc ccatgacggt
      481 gtcgagcgaa tagacggcga gggccagcca gatgcagccg aagcaggcag cctgggcggc
      541 gccgaaaggc tcgccataga cgaagacgcc gagcaggaag gccgcggagg gcgccgtgaa
      601 ctgcaggagg ccgatggtgg tgagcggcag cttacgcgcc gccgccgcga accagacgag
      661 cggcaacgcg gtgaaaaggc cagagccgag gagcagcata tcgagtcccg gtgaagcgag
      721 cgcgaaggcg ccgcgcccgg tcgcggccag ccagaggaga tagcccgcgg cgaagggtgc
      781 gaggatggtc acctcgacgg tgaagcccgc cagcgcctcg gccgctacga ccttgcgcac
      841 caggccgtag cagccgaagg agagagcgag gctcagggcg atccaaggca cgccgcggat
      901 ggcggcggcg aggatcagga cgccgaaggc ggcgatggcg caggcccaga gctgcctcgg
      961 gcgcagccgc tcgccgagca ccagcaggcc cagcgcaatg ctcaccagcg gattgaggaa
     1021 atatccgaga ctcgcggcaa gcaggtggtc gctggtgacg gcgacgacat aagtgaccca
     1081 gttgatggtg acgagcgccg ccgacagcac gagcaggccc aggctgcgcg gggtgagcac
     1141 ggttcggagc acgcgcaggc gaccgaggag ggcgaggagg gcgagagcca ggagcgccgc
     1201 ccagacgacg cggtgggcga ggatctccgc gaccggaacg gcggcgagcg ccttgatata
     1261 gaccggcagc acgaggcccc agagcccgaa ggcgccgagc gcgtagagaa gcccggcgcg
     1321 ccgctcgtct cctttgccgt ctctcattct tcgggctctt tgctgccgca gcgagacgcc
     1381 acgttggtgc cgcggcgagg cgccacgtta acctccggtc atgggagcgg ctagtgtcgt
     1441 tcgcggcgaa cgtctatgat cggtgcccgc cggaggaacg cggatggtca acatcaggca
     1501 ggctcgactc gcggatgcgc gagcgatcgg ccgcatcgaa gtcgagacat ggcgcgccac
     1561 ttatgcgggc atgctttccg accgggtgct cctctcgatg tcgaccgagc gtcagaccgc
     1621 ctcttgggcc ggcttcctgc gccatcgtcc gggcgacgtg ttcgtcgcgc agcaaccggc
     1681 cggtgtggtc ggcttcggca attgcgggcc gcagcgcgag gggagcttcg gctacggcgg
     1741 cgaagtctac accctctatg tgacgcccga tgcgcagggc acgggctgcg gccgccagct
     1801 cctcctcgcg ctcttcgccc ggctcctcca ttgcggccat cgctcggcgc tgatctgggt
     1861 gttgcgcgcc aatccggcgc ggttcttcta tgagcggctg ggcggcaagc tggtgctgca
     1921 ccgcgccatt ccggtgagcg gcgaggccgc cgaagcgctg ggctatggct ggagcgatct
     1981 ccgcgcgctc ctcgaccgcg aagcgcgaag cggcgccggc cgctcctcgc cgccgccctg
     2041 aaggcgcggc ttgcgcctgg gtcgtgcgcc gaccggttga tcgtttcttg ggtgaatcag
     2101 cgctaagctg ccgcccgctc gccgcaaccc cgcgagctca aataccagat cggcgattcc
     2161 gaggcccagg tgatggttac gcttggcctc cgtcgccacc gacggcgcgt tcaagcgcca
     2221 tccccccggc aagctcgagg acggccgagg ccttccgcgg cggccgcttc cacaccggcg
     2281 acatcggctt cgtcgacaag gacggctatc tgacgaagac tcgtcctccg cgaccttggc
     2341 gtcctcgcgt ccgctaattt atctcaagcc gcgtccgtcg taaggtcgac ttcaggcctg
     2401 cgaatcttcg ttcgcggccg ccagctcctg gagctccgtg atctccgcca tcactcggcg
     2461 cttcgagccg gccgcgtcgg cgccgaaccc cggcagcagg tagtcgtccg gcacgcgcgc
     2521 gaggtcggcc agcgcgtcgg caaagtgccc gagcc
//
LOCUS       DASYNS010000485         4903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4334226, whole genome shotgun sequence.
ACCESSION   DASYNS010000485 DASYNS010000000
VERSION     DASYNS010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4334226"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..733)
                     /locus_tag="VGP50_15115"
     CDS             complement(2..733)
                     /locus_tag="VGP50_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV8028762.1"
                     /translation="MGNSDTARSPPAHVAIIMDGNGRWAKARGLPRIAGHRHGADAVR
                     RTVTAAGEFGIAYLTLFGFSSENWKRPVVEVDDLMGLLRHYLRREIAELHRNGVRLKV
                     IGERSRLPEDIVTLITNAEALTADNLGLRLTVALSYGGRAEIVRAARTIADLAKAGAL
                     APESIDEDLFAHHLLTADMPDPDLVIRTSGEQRISNFLLWQTAYAEFVFTGTMWPDFG
                     KSDLENALSDYHGRERRYGTSLGSR"
     gene            complement(747..1268)
                     /gene="frr"
                     /locus_tag="VGP50_15120"
     CDS             complement(747..1268)
                     /gene="frr"
                     /locus_tag="VGP50_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV8028763.1"
                     /translation="MDGALEVLRKEFAGLRTGRASASLLDPVQVEAYGNTMPLAQVGT
                     VSVPEPRLIIVQVWDRGVVKATEKAIREAGLGLNPQTEGQTIRVPIPDLNEDRRKELT
                     RVAAKYAEQARISVRNVRRDGMELLKKQEKDHKLTQDQHRKLDKEVQTLTDETINKVD
                     SLLAQKDKEILQV"
     gene            complement(1318..2055)
                     /gene="pyrH"
                     /locus_tag="VGP50_15125"
     CDS             complement(1318..2055)
                     /gene="pyrH"
                     /locus_tag="VGP50_15125"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019643953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV8028764.1"
                     /translation="MPSDPIDPPVPKYRRVLLKLSGEALVGEHAYGLDPKVVTRLANE
                     VKAVHSVGVQICLVIGGGNIFRGLAGAAQGMERATADYMGMLATVINSLAMQSALERQ
                     GVVTRVLSAISMQAVCEPYIRRRAIRHMEKGRVVIFAAGTGNPFFTTDTAAALRASEM
                     ECDALLKATKVDGVYSADPNKDRTATRYERLTYLDVLSRDLQVMDATAISLARENRIP
                     ILVFSIHNEGGFADVVAGRGRFTIVNE"
     gene            complement(2143..3027)
                     /gene="tsf"
                     /locus_tag="VGP50_15130"
     CDS             complement(2143..3027)
                     /gene="tsf"
                     /locus_tag="VGP50_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEV8028765.1"
                     /translation="MAEITAALVKDLREKTGAGMMDCKRALAETSGDLEGAVDWLRKK
                     GLSAAAKKAGRVAAEGLVGAATSGKAGALVEVNTETDFVARNEAFQRYVHNLAELTLR
                     GGEDIERLKAMAFPGSARNVGDELTHMIATIGENMALRRLRRLEVKNGLVISYVHSAL
                     APGLGKIGVLVALESTGDHGKLAPVGSKLAMHVAAANPQYLDIGAVDKAVLEREREVL
                     REQTRAQGKSEDLIPKIVEGRLRKFYEDVVLLEQTYVVDGESRVKQVIEAAAKELGAP
                     VKIAGFVRFALGEGIPKE"
     gene            complement(3094..3891)
                     /gene="rpsB"
                     /locus_tag="VGP50_15135"
     CDS             complement(3094..3891)
                     /gene="rpsB"
                     /locus_tag="VGP50_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV8028766.1"
                     /translation="MAIPDFTMRQLLEAGVHFGHHTRRWNPKMAPYIFGVRNGVHIVD
                     LEQTVPMLHRALQAIRDVAASGGRVLFVGTKRQAQEVVADAAKRCGQYYVNHRWLGGM
                     LTNFKTISQSIKRLRELDERINEPTSGLTKRELLELTRDRDKLERALGGIKEMGGLPD
                     ILFIIDTNKEAIAVQEANKLKIPTVAVLDSNSDPDGVAYPIPGNDDAMRAIHLYCELV
                     SGAVLDGLQAELAATGADIGEQQELPAELPIIVEEAPAGSEEAQATA"
     gene            complement(4081..4452)
                     /locus_tag="VGP50_15140"
     CDS             complement(4081..4452)
                     /locus_tag="VGP50_15140"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEV8028767.1"
                     /translation="MPDAQITDAKELLRRVTDFGARLKSQAGAEQKEALWAATVEIQG
                     KQFDGLIVDFSPGGARIHFAAKVTTEADVALVLNELDALGATVVWQTEGKSGVQFLLA
                     PEEVAARVRRRPDVLAKRLRA"
     gene            complement(4558..>4903)
                     /locus_tag="VGP50_15145"
     CDS             complement(4558..>4903)
                     /locus_tag="VGP50_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV8028768.1"
                     /translation="SRQLLEAGQKLLVTTDVRAEEETLRLTAQRIEALDSMVAHAAAG
                     LKVFLGEIEALAPLRNLVLRESGGRGRVSVVVRVPPAREVEIALPGAFKISPSVRAAV
                     KSLPGILDVHDI"
BASE COUNT          767 a         1631 c         1602 g          903 t
ORIGIN      
        1 attagcggga gccaagagag gtgccgtagc ggcgttcgcg gccgtgatag tcgctgagcg
       61 cgttttcgag gtcgctcttg ccgaaatcgg gccacatggt gccggtgaag acgaactcgg
      121 cgtaagcggt ttgccagagc aggaagttgc tgatgcgctg ctcgccgctg gtgcggatga
      181 cgagatcggg gtctggcatg tcggcggtga gaaggtggtg ggcgaagagg tcctcgtcga
      241 tcgactcggg cgccagcgcc ccggccttcg ccagatcggc gatggtgcgg gcggcacgga
      301 cgatctcggc gcggccgccg taggaaaggg ccacggtcaa acgcaacccg aggttgtcgg
      361 cggtcagcgc ctcggcattg gtgatgagcg tgacgatgtc ctccggaagg cgggagcgct
      421 cgccgatgac cttgagccga acgccgttcc ggtgcagctc ggcgatctcg cggcgcagat
      481 aatggcgcaa caggcccatc agatcatcga cctcgaccac cggccgcttc caattctccg
      541 acgagaagcc gaaaagcgtg agataggcga tgccgaactc gcccgccgcg gtgacggtgc
      601 ggcggaccgc gtcggcgcca tggcgatggc cggcgattcg cggcagcccg cgcgccttgg
      661 cccagcgccc gttgccgtcc atgatgatcg ccacatgcgc cggcggcgac cgcgccgtat
      721 cgctattccc cattgtctcc acgcgctcag acttgaagga tttccttgtc cttctgggcg
      781 agcaggctat cgaccttgtt gatcgtctcg tcggtcagcg tctggacttc cttgtcgagc
      841 ttccggtgct gatcctgcgt cagcttgtgg tccttctcct gcttcttcaa gagctccatg
      901 ccgtcgcgtc gcacattgcg gacggagatg cgggcctgct ccgcatattt ggcggcaacc
      961 cgcgtcaact ccttgcgccg gtcctcgttg aggtcgggga tcggcacgcg gatcgtctgg
     1021 ccctcggtct gcgggttgag cccgagcccg gcctcgcgga tcgccttctc ggtggccttg
     1081 acgacacccc tgtcccagac ttggacgatg atcaggcgcg gctcgggcac gctcacggtc
     1141 cccacctgcg caagcggcat ggtattgcca taggcctcga cctgcacggg atcgaggagg
     1201 cttgccgagg cgcgccccgt gcgcagcccg gcgaactcct tgcgcagcac ctcgagagcc
     1261 ccgtccatgc ggcggcgcag gtctttgacg atgggatctt cgctcacttc tcgattctca
     1321 ctcgttgaca atggtgaagc ggccccgtcc ggcgacgacg tcggcgaagc caccctcatt
     1381 gtggatcgaa aagaccagga tcggaatgcg gttctctcgc gccagcgaga tggccgtcgc
     1441 gtccatcacc tggagatcgc gcgagagaac gtcgaggtag gtcaggcgct catagcgcgt
     1501 cgccgtccga tccttgttcg ggtcggccga gtagacgccg tcgaccttgg tcgccttgag
     1561 gagagcgtcg cattccatct cggaggcgcg cagcgccgcc gcggtgtcgg tggtgaagaa
     1621 cgggttgcct gtaccggcag cgaagatcac caccctccct ttctccatgt ggcggatggc
     1681 gcggcggcgg atatagggct cgcacaccgc ctgcatgctg atggccgaca acacgcgcgt
     1741 cacgacgccc tggcgctcga gcgcgctctg catggcgagc gaattgatga cggtggccag
     1801 catgcccatg tagtcggccg tcgcccgctc catgccctgc gccgcaccgg caaggccgcg
     1861 aaagatgttg ccgccgccga tcaccaggca gatctggacg cccaccgagt gcactgcctt
     1921 cacttcgttg gcgagtcgcg tgaccacttt cggatcgagg ccgtaagcgt gctcgccgac
     1981 caacgcctcg cctgacagct tcaacaggac acggcggtat ttggggacgg gggggtctat
     2041 cgggtccgat ggcatggcgc cgacatatat acgacgcgag agcgggtctc gccactcaat
     2101 cccctctcct gcccttggcg ggggtagcct tcgcgcgttc gcctactctt tcgggattcc
     2161 ctcgcccagc gcgaagcgga cgaaaccggc gatcttgacc ggcgcaccca gctccttggc
     2221 ggcggcctcg atcacctgct tgacgcggct ctcgccgtcg acgacgtagg tctgctcgag
     2281 cagcaccaca tcctcgtaga acttgcgcag gcggccctcg acgatcttcg gaatgagatc
     2341 ctcgctcttt ccctgtgcac gggtctgctc gcgcagcacc tcgcgctcgc gctcgagcac
     2401 ggctttgtcg acggcgccga tgtcgagata ttgcgggttc gccgcggcca catgcatggc
     2461 gagcttgctg cccaccggcg cgagcttgcc gtgatcgccc gtcgattcga gcgccaccag
     2521 cacgccgatc ttgccgagtc cgggcgccag cgcgctgtgc acataagaga tgaccagccc
     2581 gttcttcacc tcgagccggc ggaggcggcg cagcgccatg ttctcgccaa tagtggcgat
     2641 catatgcgtg agttcgtcgc cgacgttgcg cgcgctgccc gggaacgcca tcgctttgag
     2701 ccgctcgatg tcctccccgc ccctgagcgt gagctcggcg aggttatgga catagcgttg
     2761 gaacgcttcg ttccgcgcga cgaaatcggt ctcggtattg acctcgacca gcgcgcccgc
     2821 cttgccgctg gtggcggcgc cgacaagacc ctcggcggcg acgcggcccg ccttcttcgc
     2881 ggccgcggaa agtcccttct tgcgcagcca gtcgacggcg ccctcgaggt cgccgctggt
     2941 ctccgccaag gcgcgtttgc aatccatcat gccggcgccg gtcttctcgc gcagatcctt
     3001 gaccagcgca gcggtgatct cggccatagc tcttctcctg caaaagaacg gcggcgagaa
     3061 cgtcgccgcc gttcccactc tctcgcgaac gcgctaggcg gtcgcctgtg cttcctcgga
     3121 gccggcaggc gcctcttcga cgatgatcgg cagctccgcc ggcagctcct gctgttcgcc
     3181 gatatcggcg ccggtagcgg caagctcggc ctgcaagccg tccagaaccg cgcccgatac
     3241 cagctcgcaa taaaggtgaa tggcgcgcat cgcgtcgtca ttgccgggaa ttggataagc
     3301 gacgccgtcg ggatcggagt tgctgtcgag cacggccacg gtcggaatct tcagcttgtt
     3361 cgcctcctgg acggcgatcg cttctttgtt ggtatcgatg atgaagagga tgtcgggcag
     3421 cccgcccatc tccttgatgc cgcccagcgc gcgctcgagc ttgtcgcgat cgcgggtgag
     3481 ctccaggagc tcgcgcttgg tgagtcccga tgtcggctca ttgatgcgct cgtcgagctc
     3541 gcgcaggcgc ttgatcgatt gcgagatggt cttgaagttg gtgagcatac cgccgagcca
     3601 gcggtggttg acatagtact ggccacagcg cttggcggcg tcggcgacga cctcttgcgc
     3661 ctggcgcttg gtgccgacga agaggacgcg tccgccgctc gccgcgacgt cgcggatcgc
     3721 ctgaagcgcc cggtgcagca tcggcaccgt ctgctcgagg tcgacgatgt ggacaccgtt
     3781 gcggacaccg aagatgtaag gcgccatctt cgggttccag cgccgcgtat ggtggccgaa
     3841 atgcacgccc gcttccaaaa gctggcgcat ggtgaaatcg gggattgcca tcttccttct
     3901 ccggttgggc cgccgcgggg gatcatcgat cgccgatcga caccggagcg gccgcttccg
     3961 tatccggaaa cgaccgcgtc cccgcgtgag gtctggggtt agaagcgcct taaatagtcc
     4021 gattgcgccg atttagcaag agcgcgtcgt tactctcccg cgaagagcgg gagggggccg
     4081 tcaggcgcgc agacgcttcg cgaggacgtc gggacggcga cggacgcgcg ccgccacctc
     4141 ttcaggggcc aggaggaatt gcacgccgct cttgccctcg gtctgccaga cgacggtcgc
     4201 gccgagcgca tcgagctcgt tgaggaccag cgcgacatcg gcttccgttg tcaccttggc
     4261 ggcaaaatgg atacgtgccc cgccgggcga gaaatcgacg atcaggccgt cgaactgctt
     4321 gccctggatc tccaccgtcg cagcccagag cgcctccttt tgctcggccc cggcctgact
     4381 tttgagccgc gcgccgaagt cggtgacccg gcggagcagc tccttggcat cggtgatctg
     4441 tgcgtcaggc atgatttcgt cccggagacg cgcatccgat cggcgcactg aaactttaac
     4501 cgccaatcct taatcgacgc tctccgatac ccggcatttt cggcgaacgc cggccactca
     4561 gatgtcgtgg acatcgagga ttcccggtaa cgatttgacg gccgcgcgca cgctggggct
     4621 gatcttgaac gcgccgggga gcgcgatctc gacctcgcgg gcgggcggca cgcggacgac
     4681 cacgctgacg cgaccgcgcc cgccgctctc gcgcaggacc aggttgcgca gcggcgccag
     4741 cgcctcgatc tcgcccagaa acactttgag cccggccgcg gcatgggcga ccatgctgtc
     4801 gagcgcctcg atgcgctgcg ccgtgaggcg aagggtctcc tcctcggcgc gcacatcggt
     4861 cgtcaccagc agcttctgtc cggcctcgag gagctgccgg ctc
//
LOCUS       DASYNS010000486         5946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3087525, whole genome shotgun sequence.
ACCESSION   DASYNS010000486 DASYNS010000000
VERSION     DASYNS010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3087525"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VGP50_15150"
     CDS             <1..598
                     /locus_tag="VGP50_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028769.1"
                     /translation="LIAVSLLALLMALLTAGLRAGTHNMARQSERLDRSSRNVLVETF
                     LRNELADARPFSTAGDSRSSTIAFTGREDGVTFVGPSPASVATGGLQMLSVDFAKDGP
                     ARGEVVADWQPYGAGTGAGANPRRSLLLGHVERAIFAYFGAEPGEAPSWHSDWENKTV
                     LPSLVRLSVEFSDGELMPDLVIALRLADDTNGQAGARQ"
     gene            595..2331
                     /locus_tag="VGP50_15155"
     CDS             595..2331
                     /locus_tag="VGP50_15155"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEV8028770.1"
                     /translation="MSLQTALSLHQQGRLAEAESLYQALLDADRDHFDVLHALGILRC
                     QQRRYEEARGLFLHALRQKPGSPEAHYNLATALEGLKHPEEAVLHYREALALRPDFYP
                     AHNNLANVLKALDQREEAIVHYRRALAIRPDFAIAHNNLANTLQALGRRDEAETHYRR
                     AIGFKPDYAEAHNNLGNVLRVLGRGEEAVQCYRRALAFEPRYLEAHNNLGNALKELGQ
                     FEEAAASFKKALELDPKCVAAYHDLALATKVKADEPWLAAMEKLASDSDGLKPRDRAG
                     LHFALAKTSEDLERHDEAFRHMLAANALKRREIAYDEARTFARFARIRELVTAELLRQ
                     KAGSGDPSRAPIFILGMPRSGTTLVEQILASHPDVFGAGEIDDLTLLAEAMPYPEGLP
                     EASADQLRALGAGYVAGVRARAGAALWITDKMPSNFYYVGLIRLALPNARIIHTRRDP
                     IDTCLSCFSKLFVGVAQPFSYDLRELGRYWRQYDALMAHWRQVLPEGAMLEIPYEELV
                     ADLETQARRLVAYCGLDWNDACLAFHETRRAVRTASAVQVRHPVYKSAVGRWRIYEEQ
                     LAPLIEELKIPR"
     gene            2328..4466
                     /locus_tag="VGP50_15160"
     CDS             2328..4466
                     /locus_tag="VGP50_15160"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8028771.1"
                     /translation="MSGAPRAVQIYQEAVAHQAAGRLDAAERCYREALGLHPDYPQAH
                     HGLASLLGRLDRLEEAVAHYREALRLRPDWAVAHYNLGHLLNRLGRIEEAAECYRQAL
                     RFKRDFPEALNNLAHILNRLARYEEAERSCRAALRLKPDNPAAHNNLGNALSGLSRFA
                     EAEASYRAALRLNPDLPETQRNLGDVLGTLRRYEESLPCYREALRLRPGMADAWSNLG
                     LTLTALGEPEEAERCYDEALRLKPDFAAAQINRCIGRLRIVYRDEAEIERARATYAAD
                     LEAVCRLEGPEALAAAGKPPFLLAYQGRVDRALQARYGGFVARAMATLHPAWASAPEV
                     APPAAGEAIRVGILSAYFYAHSNWKIPIKGWLQALDRRRFRLFGYHIGQRRDEETDLA
                     RKLCHRFVEGLPSVERWAERIRADRLHVLLIPGIGMDDMTLRLAALKLAPVQATSWGH
                     PDTTGLPTIDDYLSSALMEPPGGDAHYTERLVRLPNLSIWYEPREVASEHVTRAELGL
                     PEDAILYWCCQSLYKYLPRYDDVFARIAAAVPGARFVFIRYPHGDKVTAVFRERLAAV
                     FAKAGSDARQHCVFLPPMSAARFAAVGRLADVFLDSLGWSGCNSTLEALAGDLPVVTM
                     AGELMRGRHSAAILTMLEMPELIAANADAYVALAVLLGQDPGRRSTLAQGIARQKSRL
                     YRDRTAIDGLSAYLEDAAHRRSHSMITPLY"
     gene            4797..>5946
                     /locus_tag="VGP50_15165"
     CDS             4797..>5946
                     /locus_tag="VGP50_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028772.1"
                     /translation="MVSFSKNLLAACSTAALAAAMAAPAAPAAAQSNPALQVNATTGV
                     PSGQPSNFEGSAAFGVSDPGVRGGAVDAGGALPGLNTDELNFFFNARDRFQEVDSVNG
                     TVADHDAGLPSPETGAGLGPSFNGNACAQCHVFPAVGGTSPPVTSPQVTLATLDGAKN
                     TVPPFVANGGPVREARFVNDAAGNPDGGVHDLFVITGRTDATNQPNANTGTNTTCTVA
                     QTNFPNQIAANNVIFRIPTPTFGIGMIENVGELTLAADAAAAGTGAANLGITAGNFNH
                     SGNDGTITRFGWKAQNKSGLVFAGEAYNVEQGVTNENFQNERTGQFQSGGNNDSNAAI
                     TNCLFNATPEDATALENTRNTNSPASDFSSDIVNFAGFMRLSNQPTPAR"
BASE COUNT          983 a         2139 c         1879 g          945 t
ORIGIN      
        1 cctcatcgcg gtcagcctgc tcgcgttgct gatggctctg ctgacggccg ggctccgcgc
       61 cggaacgcac aacatggcgc ggcagtccga gcggctcgac cgctcctcgc gcaatgtgct
      121 ggtggagacg tttctgcgca atgagctcgc cgacgctcgt cccttcagca cggccggcga
      181 ttcgcgctcg agcacgatcg cgtttaccgg ccgcgaggac ggcgtgacgt tcgtcggccc
      241 gtcgccggcg agcgtcgcca ccggcgggtt gcagatgctc tccgtcgatt tcgcgaagga
      301 cggccccgcg cgcggcgaag tcgtcgcgga ctggcagcct tacggcgcag gcactggcgc
      361 gggcgcaaac ccccggcgca gccttctgct cggccatgtc gagcgcgcca tcttcgccta
      421 tttcggcgcc gagcccggcg aggcgccaag ctggcattcg gactgggaga acaagacggt
      481 gctgccgtcc ctggtgcggc tctcggtgga attctcggat ggcgagctca tgcccgacct
      541 ggtgatcgcg ctgcgcctcg ccgacgacac gaatggacaa gcgggggctc ggcaatgagt
      601 ctgcagacgg cgttgtcgct tcatcagcag gggcggctcg cggaagccga gtcgctctac
      661 caggcgctcc tcgatgccga ccgggaccat ttcgatgtgc tccacgcgct cggcatcctg
      721 cgttgccagc agcgccgcta tgaagaagcg cgcggcctct tcctccacgc gctgcgccag
      781 aagcccggtt ccccggaggc gcattacaat ctcgcgacgg cgctcgaagg gctgaagcac
      841 cccgaggagg cggtgcttca ctatcgcgag gcgctggcgc tccgccccga tttctatccc
      901 gcccacaaca atctcgccaa tgtgctgaag gccctcgacc agcgcgagga agcgatcgtg
      961 cattaccgtc gggcgctggc gatccggccg gatttcgcca tcgcccacaa caacctcgcc
     1021 aatacgctcc aggcgctggg ccgccgcgac gaggcagaga cgcactatcg acgcgccatc
     1081 ggcttcaagc ccgattatgc cgaggcacac aacaatctcg gcaatgtgct gcgcgtgctc
     1141 ggccgcggcg aggaagcggt gcagtgctac cgccgggccc tcgccttcga gccgcgctat
     1201 ctcgaggccc acaacaatct cggcaacgcg ctcaaggagc tcggccagtt cgaagaggcc
     1261 gcggcctctt tcaagaaagc gctcgagctc gaccccaaat gcgtcgccgc ttatcacgac
     1321 ctggcgctcg cgacgaaggt caaggcggac gagccctggc tcgccgccat ggagaagctc
     1381 gcttccgaca gcgacggcct caagccgcgc gaccgcgccg ggcttcattt cgcactcgcc
     1441 aagacgtccg aggatctcga gcgccatgac gaagccttcc gccacatgct cgccgccaac
     1501 gcgctgaagc ggcgcgagat cgcctatgac gaggcgcgga cattcgcgcg cttcgcgcgc
     1561 atccgggagc tggtcacggc ggagcttctg cgacagaagg cggggagcgg cgatccttcg
     1621 cgggcgccga tcttcattct cggcatgccg cgctcgggca cgacgctcgt cgaacagatc
     1681 ctggcgagcc accccgacgt attcggcgcc ggcgagatcg atgacctgac gctgctcgcg
     1741 gaagccatgc cttaccccga aggactgccg gaagcgagcg ccgaccagct gcgcgccctc
     1801 ggcgccggct atgtcgcggg cgtgcgggcg cgcgcgggcg ccgcgctctg gatcaccgac
     1861 aagatgccgt cgaacttcta ttatgtcggc ctgatccgcc tggcgctccc caacgcccgc
     1921 atcattcaca cgcggcgcga ccccatcgat acctgcctct cctgcttctc gaagcttttc
     1981 gtcggcgtgg cgcagccctt cagctacgac ctgcgcgagc tcggacgcta ttggcgccaa
     2041 tacgacgcgc tcatggcgca ttggcgccag gtcctgccgg aaggcgcgat gctcgagatc
     2101 ccgtacgagg agctcgtcgc cgacctcgag acccaggccc gccgcctcgt cgcctattgc
     2161 gggctcgact ggaacgacgc ctgtctcgcc ttccacgaga cgcgccgcgc cgtgcgcacg
     2221 gcgagcgccg tgcaagtgcg ccatcccgtg tacaagagcg ccgtcgggcg ctggcgcatc
     2281 tacgaagagc agctggcacc gctcatcgag gagctcaaga tcccgcgatg agcggcgcgc
     2341 cccgcgccgt ccaaatctat caagaagccg tcgcccatca ggcggcggga cggctcgatg
     2401 ccgccgagcg ctgctatcgc gaagcgctcg gcctccaccc ggattatccg caggctcatc
     2461 acggcctcgc cagtctcttg ggccggctcg atcggctcga ggaggcggtg gcgcattacc
     2521 gcgaggcgct ccgcctccgt cccgactggg cggtggcgca ttacaatctg ggccacctgc
     2581 tcaaccgcct tggccgcatc gaggaggccg ccgaatgcta tcgccaggcc ctgcggttca
     2641 agcgcgattt tcccgaggcg ctgaataatc tcgcccacat cctgaaccgc ctggcgcgct
     2701 acgaggaagc ggagcggtcc tgccgcgccg ctttgcgcct caagcccgac aacccggccg
     2761 cgcacaacaa tctcggcaac gccttgagcg gattgtcccg cttcgccgag gccgaggcct
     2821 cgtaccgcgc ggcgctccgt ctcaacccgg atctgccgga gacgcagcgc aatctcgggg
     2881 acgtcctcgg cacgctgagg cgctacgagg aatccctgcc ctgctatcgt gaagcgctgc
     2941 gcttgcggcc cggcatggcc gacgcctgga gcaatctggg cttgacgctg acggcgctcg
     3001 gcgagcccga ggaggcagag cgctgctatg acgaggcgct ccgcctcaag cccgatttcg
     3061 ccgcggccca gatcaaccgc tgcatcggcc ggctccgcat cgtctatcgc gacgaggcgg
     3121 agatcgagcg ggcgcgcgcc acctatgccg ccgatctcga agcggtctgc cgcctcgaag
     3181 gtccggaagc gctggccgcc gccggcaagc cgcccttcct cctggcctat cagggacgcg
     3241 tcgaccgcgc gcttcaggca cgctatggcg gcttcgtcgc ccgcgccatg gcgacgcttc
     3301 accccgcctg ggcctcggcc cccgaggtcg cgccgcccgc cgccggcgaa gccatccgcg
     3361 tcggcatcct ctcggcgtat ttctatgccc actccaactg gaagatcccg atcaagggct
     3421 ggctccaggc gctcgaccgg cggcgcttcc gcctcttcgg ctaccacatc ggccaacggc
     3481 gcgacgaaga gacggacctc gcccggaagc tctgccatcg cttcgtcgag ggtttgccga
     3541 gcgtcgagcg ctgggcggag cggatccgcg ccgaccgcct gcatgtgctg ctgatccccg
     3601 gcatcggcat ggacgacatg acgctgcgcc tcgccgcact gaagctcgcg ccggtgcagg
     3661 cgacgtcctg gggccatccc gatacgaccg ggcttccgac catcgacgac tatctcagca
     3721 gcgcgctgat ggagccgccc ggcggcgacg cgcactatac cgagcggctg gtccgcctgc
     3781 cgaacctgtc gatctggtac gagccgcgcg aggtcgcgtc cgagcacgtg acgcgagccg
     3841 agctcggctt gcccgaggac gccatcctct attggtgctg ccagtcgctc tacaagtatc
     3901 tgccgcgcta cgacgacgtc ttcgcacgca tcgccgcggc agtgccgggc gcgcgcttcg
     3961 tcttcatccg gtacccccat ggggacaagg tcacggcggt ctttcgcgaa cgcctcgccg
     4021 ccgtcttcgc caaggccggc agcgacgccc ggcagcactg cgtgttcctt ccgccgatga
     4081 gcgcggcgcg ctttgccgcc gtcggacggt tggccgatgt gttcctcgac agcctcggct
     4141 ggtccggctg caactcgacc ttggaggcgc tggccggcga ccttccggtc gtgaccatgg
     4201 cgggtgagct gatgcggggc cgccacagcg ccgccattct gacgatgctg gagatgccgg
     4261 agctgatcgc cgccaacgcc gacgcctatg tcgcgctggc cgtactgctt ggtcaggacc
     4321 cgggccggcg cagcactctg gcacagggta tcgcgcgaca gaaaagccgg ctctaccgcg
     4381 accggacggc cattgacggg ctgtccgcct atctcgagga tgccgcccat cgtcgtagtc
     4441 actcaatgat tacaccgctt tattgacgac gggctgccga agacttggtt aacattggat
     4501 ccgctgtaga tttgcatcgg tttattgttc tcgacggccc gatacatcac ttgaaatgtg
     4561 aagtttcttg ttgcacccaa ccataggcgg ggttagtctt gttccaaggt aacgacaacg
     4621 ttaccgctgg ataacgcaga agttacaatg tcccggggcg ccttgggagg gaggccccgc
     4681 tcaacgagag agatggaaac gaggcccagg gcccggctca cgcggaggcg catgccttta
     4741 gcggacgccc ccttgcgagt cgggatcgat caatgaactt tcaggaggac gttacaatgg
     4801 tgagcttttc caagaacctg cttgcggcct gcagcacggc agcgcttgcc gccgcgatgg
     4861 ctgcgccagc tgctccggcc gctgcccagt ccaatccggc tctgcaagtg aatgcgacga
     4921 cgggcgtgcc gtcgggtcag ccatcgaatt tcgagggctc cgccgcgttc ggcgtgagcg
     4981 atccgggcgt gcgcggcggc gcggtggacg ccggcggcgc cctgccgggg ctcaacaccg
     5041 acgagctcaa tttcttcttc aatgcgcgcg accgcttcca ggaagtggac tcggtgaacg
     5101 gcaccgtcgc cgaccatgac gcgggcctgc cgagcccgga gaccggcgcc ggcctcggac
     5161 cgtccttcaa cggcaatgcc tgcgcccagt gccacgtctt cccggccgtc ggcggcacga
     5221 gcccgccggt caccagtccg caggtgacac tcgcgactct ggacggcgcc aagaacaccg
     5281 tgccgccctt cgtcgccaat ggaggcccgg tccgcgaggc acgcttcgtc aacgacgccg
     5341 cgggcaaccc cgatggcggc gtgcatgacc tcttcgtcat caccggccgc accgacgcca
     5401 ccaaccagcc caacgccaac acgggaacca acaccacctg cacggtggcg cagaccaact
     5461 tcccgaacca gatcgccgcc aacaacgtca tcttccgcat cccgacgccg accttcggca
     5521 tcggcatgat cgagaatgtc ggcgagctga ccttggcggc cgacgcggcg gcggccggca
     5581 ccggcgcggc caatctcggc atcaccgccg gcaacttcaa tcacagcggc aatgacggca
     5641 cgatcacccg cttcggctgg aaggcgcaga acaagtcggg tctggtgttc gccggcgagg
     5701 cctacaatgt cgagcagggc gtcaccaacg agaacttcca gaacgagcgg accggccagt
     5761 tccagtcggg cggcaacaac gacagcaatg ccgccatcac caactgcctc ttcaacgcga
     5821 cgccggagga cgcgaccgcc ctcgagaata cccgcaacac caacagcccg gcctccgact
     5881 tctcgtcgga catcgtcaac ttcgccggct tcatgcggct ctccaaccag ccgacgccgg
     5941 cgcggc
//
LOCUS       DASYNS010000487         6575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3244822, whole genome shotgun sequence.
ACCESSION   DASYNS010000487 DASYNS010000000
VERSION     DASYNS010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6575
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3244822"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2866
                     /locus_tag="VGP50_15170"
     CDS             <1..2866
                     /locus_tag="VGP50_15170"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF010706.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HEV8028773.1"
                     /translation="DTAGNRLLASLFGNSPFLTQCCLTEPALLMRLGQEGPEATFAEL
                     VLRLNRLLAPAADRAVLMRTLRQAKRRAALLIAIADIAGWWPLDRVTGALSDLAEAAL
                     GALVRHLLAAAAAAGEIVLQDPEQPEHESALIVLGVGKLGARELNYSSDVDLIILFDG
                     EKSRYTGSGSPPQFFAGLARNLARLMAERAADGYVFRTDLRLRPDPGSTLPAMSVLAA
                     LTYYESAGQNWERAALIKARPVAGDRAAGAGFLTELRPFLWRKHLDFAAIQDIHSIKR
                     QIDAHRGGGRIAVAGHDVKLGRGGIREIEFFAQTQQLIWGGRFPALRSSGTVAALAAL
                     AAAGRISAETAAELTEAYRFLRGVEHRLQMIDDAQTHAIPADPTAIQHLAIFLGFPSR
                     DAFAQALLRRLATVEGRYAKLFEEAPTLSGPGNLVFTGTEDDPDTLKTLAALGFTDPR
                     AAAGMIRGWHHGRYRAMRSQRARELLTELVPGLLRAVAASPNPDLALVRFDQFLSRLP
                     AGVQLFSLFLRNGVLLDLVAEIMGAGPRLAEQLARRPALLDGVLSGGFFGAPPPRADL
                     IQELDALLAPARHYEEVLDLVRRWVGDRKFQIGVQLLRDRIDGEEAGAALADVAETAI
                     AGLLPPVAAAFAQSHGGVPGGALVVLGLGKLGGREMTVTSDLDLILIYDAPETVEAST
                     GPRPLAVSTYYARLCHRLIDALTALTPEGHLYDVDMRLRPSGTKGPVATSLAAFRRYH
                     GELAWTWEQMALTRARVVAGPEALGGAVMARVHAVLTEPRDGDRLVVDVADMRQRMAE
                     QHRAPGPWDVKHRRGGLVDIEFIAQYLQLREAPAQPQLLRQNTSAALAALADGGALDA
                     VAAEDLVETLDLWRNVQGLLKLTVEEPFDEAAASPALKAHLARGAGAVDFARLKADMD
                     AAAQRVLARYEALIEAPARAARARRATVPPSPSSAKEQSP"
     gene            2863..3327
                     /gene="bcp"
                     /locus_tag="VGP50_15175"
     CDS             2863..3327
                     /gene="bcp"
                     /locus_tag="VGP50_15175"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEV8028774.1"
                     /translation="MSIEAGDKAPDFTLPTDGGGKVALAKLRGQKVVLYFYPKDDTSG
                     CTAEACGFRDSFPKFGKIEATVIGISRDTVASHDKFKKKYELPFTLASDEKGEVTERY
                     GVWVEKSMYGRKYMGIERATFLIDEKGVVKGVWRKVKVPGHVEAVLAAAKAL"
     gene            3366..4130
                     /locus_tag="VGP50_15180"
     CDS             3366..4130
                     /locus_tag="VGP50_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEV8028775.1"
                     /translation="MLEAAAPADKIALSREAAAAWRSGALAAVGEAAPPDRPARPARP
                     LLLPPRDMPRRRSGGSHAHRIALLHAIAHIELNAIDLAWDLVARFAREDLPRAFFDDW
                     VAVAAEEAKHHALLAARLAALGAAYGDLPAHDGLWEAAQATAHDLLARLAVVPLVLEA
                     RGLDVTPGMIERLRRFGDDESAAVLQVIYDDEIGHVAAGRRWFEWLCRRRGVPPAATY
                     QALVRTHFKGALKPPFNRAGRDAAELPAAFYEPLAT"
     gene            4316..5746
                     /locus_tag="VGP50_15185"
     CDS             4316..5746
                     /locus_tag="VGP50_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028776.1"
                     /translation="MDDGKHAGHRALADLFAYPFMSCLIERRTRRIARGTSIDAGPLS
                     YQSSNKPAPLTKLEEAILIVSTGVTGLTTHDGPLVRPSDNGKELGTPFLNITARTASS
                     ADDCQATSFFVINDDGIFLLKQPKGRDALQALKDLPPKWSDWKEKDWIDAAESVKVRV
                     SNRRLDFPRDFPYYLGWNRQISNAPGSTLFFPVVDCTWQYINAILILLSEPDGKRPLV
                     IDDFRKFHPKGLIEIAAWLGSKLGITEEIPYQPIGGLKWVRNGFVNKDNPGPLGLTRT
                     LRTDYESFFALQNLMLLGQALGVGGWIHSSVFPPYIYETDPSKGWYGLGFRMVTPKKL
                     SPMPPIPASQPNPVGIDGILEGLCPPYVKSMDEAVDRVIEAKYGARGSYGDADMFSRS
                     YRDPKMGAEYLRQASHFSDRAIGYTKEICAYLWETYGRFPAHVDAFHTPGMWVQFSHL
                     EMEYYDRFFDPRQFTRQAQHDALWHR"
     gene            5774..>6575
                     /locus_tag="VGP50_15190"
     CDS             5774..>6575
                     /locus_tag="VGP50_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028777.1"
                     /translation="MLRALQIGANIVAALFLAAAVGLVLIERSWVDPEPANPRDFFLQ
                     GSTGTEIMPLAVFQVLPTLFPEEFQPAGPAAGDWVDQFGFLRGDPNVNEGLPYGINIS
                     HYRPKSGAPSPIAFVGFNCAICHSAKVHTGDDNGPVILGMGNSAIDLVGFGDAVKSSL
                     LDETRLTVSSIASTYETVTHKKLGLVDKLVIDVWLSGARTAIRSDFPMRGTPFSGPDV
                     RSAALMGSGPARNEPIKETVRFLIDETPAPDGGYSKIPCLYRQDRREWA"
BASE COUNT         1017 a         2355 c         2147 g         1056 t
ORIGIN      
        1 cgacacggca gggaaccggc tgcttgcatc gcttttcggc aacagcccgt tccttaccca
       61 atgctgtctc acggagccag ccctcctgat gcgcctcggc caggagggcc cggaggctac
      121 tttcgccgaa ctcgtcctgc ggctcaatcg ccttctcgcc cccgccgcag acagggccgt
      181 cctgatgcga acgctgcgtc aggcgaagcg gcgcgcggcg ctcctcatcg ccatcgccga
      241 catcgcggga tggtggccgc tcgaccgcgt caccggcgcg ttgagcgacc tcgccgaggc
      301 ggcgctgggc gcgctggtgc ggcatcttct cgcagccgcg gcggccgccg gcgagatcgt
      361 gctccaggat cccgagcagc ccgagcacga gagcgccctc atcgtgctcg gcgtcggcaa
      421 gctgggcgcg cgcgagctca actattcgag cgacgtcgac ctcatcatcc tcttcgatgg
      481 tgaaaaaagc cgctacacgg gaagcgggag cccgccgcag tttttcgccg gcctggcgcg
      541 caacctcgcc cgcctcatgg ccgagcgtgc ggccgacggc tatgtcttcc gcacggatct
      601 gcgtttgagg cccgatcccg gctcgacgct cccggcaatg tcggtgctgg ccgccctcac
      661 ctattatgag agcgccggcc agaactggga gcgcgccgcc ctcatcaagg cgcggcccgt
      721 ggcgggcgac cgggccgccg gcgccggatt cctgacggag ctccggccgt tcctctggag
      781 gaagcacctc gacttcgccg ccatccagga catccactcg atcaagcgcc agatcgacgc
      841 ccatcgcggc ggcgggcgca tcgccgtggc cgggcatgac gtgaagctcg gccgcggcgg
      901 catccgcgag atcgagttct ttgcccagac ccagcagctc atctggggcg gccggttccc
      961 ggcgctgcgc agctccggca ccgtggcggc gctcgcggcg ctggccgcag cggggcgcat
     1021 cagcgccgag acggcggccg agctgacgga agcctatcgc ttcctgcggg gcgtcgagca
     1081 ccggctgcaa atgatcgacg atgcgcagac ccatgcgatc cccgccgacc cgaccgcgat
     1141 ccagcacctc gcgatcttcc tcggttttcc ctcgcgcgac gcgttcgccc aggcgctgct
     1201 gcgccggctt gccacggtcg agggccgcta cgccaagctc ttcgaagagg cgccgacgct
     1261 atcggggccg ggcaacctcg tctttaccgg gaccgaggac gatcccgaca cgctgaagac
     1321 gctcgccgcg ctgggcttta ccgatccgcg cgccgccgcc ggcatgatcc gcggctggca
     1381 tcatgggcgc taccgcgcca tgcgcagcca gcgggcccgc gagctcctga ccgagctggt
     1441 gccggggctg ctgcgcgccg tcgccgcctc tcccaacccc gatctggcgc tcgtccgctt
     1501 cgaccagttc ctgtcgcggc tgccggcggg cgtgcagctc ttctcgctct ttctgcgcaa
     1561 cggcgtgctg ctcgatctcg tggccgagat catgggcgca gggccgcggc tggcggaaca
     1621 gctggcgcgg cgtccagccc tgctcgacgg cgtgctgagc ggcgggttct tcggggcgcc
     1681 gccgccccgc gcggacctca tccaggagct cgacgcgctg ctcgcgccgg cgcggcatta
     1741 cgaggaagtg ctcgacctcg tccggcgctg ggtcggcgac cgcaagttcc agatcggcgt
     1801 gcagctcctg cgcgacagga tcgacggcga ggaggccggc gccgctctcg ccgatgtcgc
     1861 cgagaccgcg atcgccgggc tgctgccgcc ggtcgccgcc gctttcgccc agagccatgg
     1921 cggcgtgccg ggaggcgcgc tcgtcgtgct cgggctgggc aagctcggcg gccgcgagat
     1981 gacggtgacg tccgatctcg acctcatcct catttatgac gcgcccgaga ctgtcgaagc
     2041 ctcgaccggg ccgcggccgc tcgcagtctc cacctattac gcgcggctgt gccaccgcct
     2101 catcgacgcg ctgacggcgc tcacgcccga gggccatctc tacgacgtcg acatgcggct
     2161 caggccctca ggcactaaag ggccggtggc cacgagcctc gccgcctttc gccgctatca
     2221 tggcgagctc gcctggacct gggagcagat ggcgctgacg cgcgcccgcg tcgtcgccgg
     2281 acccgaggcg ctcggtgggg ccgtgatggc gcgggtgcac gcggtgctga cggagccgcg
     2341 cgacggcgat cgcctggtgg tcgacgtcgc cgatatgcgc cagcgcatgg ccgagcagca
     2401 tcgcgcgccg gggccctggg acgtgaagca tcgccgcggc gggctcgtcg acatcgagtt
     2461 catcgcccaa tatctgcagc tccgcgaggc gccggcgcag ccgcaactgc tgcgccagaa
     2521 cacgagcgcg gcgctggcgg cgctggccga cgggggcgcg ctcgacgccg tcgcggccga
     2581 ggacctcgtc gagacgctcg acctctggcg caatgtgcag gggcttctga agctcaccgt
     2641 cgaggagccc ttcgacgaag cggcggcgtc gcccgcgctc aaggcgcatc tggcacgggg
     2701 cgccggcgcg gttgacttcg cgcgtctcaa ggcggatatg gatgccgccg cccagcgcgt
     2761 cctcgcccgc tacgaagcgc tcatcgaggc gccggccaga gcggcgcgtg cgcgacgggc
     2821 gaccgttcct ccctcccctt cttccgccaa ggagcaatcc ccatgagcat cgaggccggc
     2881 gacaaggcgc cggatttcac cttgccgacc gatggcggcg gcaaggtcgc gctcgcgaag
     2941 ctgcgcggcc agaaggtcgt gctctatttc tatcccaagg acgacacctc gggctgcacc
     3001 gccgaggcct gcggctttcg cgacagcttc ccgaaattcg gcaagatcga agcgacggtg
     3061 atcggcatct cgcgcgacac cgtcgcgtcc cacgacaaat tcaagaaaaa gtacgagctg
     3121 cccttcaccc tcgcctccga cgagaagggc gaggtcaccg agcgctacgg cgtctgggtc
     3181 gagaagagca tgtatggccg gaaatacatg gggatcgagc gcgcgacctt cctcatcgac
     3241 gagaaaggcg tggtaaaagg cgtctggcgc aaggtcaagg tgcccggcca tgtcgaggcg
     3301 gtgctcgccg ccgccaaggc gctctgatcg cgcccgccac gacgctcgcc gccgccgccg
     3361 cggcggtgct cgaagcggcg gcgcccgccg acaagatcgc gctgagccgg gaagcggccg
     3421 cggcgtggcg cagcggtgcg ctcgccgctg tcggcgaagc cgctccgccg gatcgcccgg
     3481 cgcggccggc gcgccctctc ctcctgccgc cgcgcgacat gccgcggcgc cgcagcggcg
     3541 ggtcgcacgc ccatcgcatc gcgctgctcc acgcgatcgc gcatatcgag ctcaatgcca
     3601 tcgacctcgc ctgggatctc gtcgcgcgct tcgcccgcga ggatctgccg cgcgccttct
     3661 tcgacgactg ggtcgccgtc gccgccgagg aagcgaagca ccatgcgctg ctcgccgcgc
     3721 gtcttgcggc gctcggtgcc gcttatggcg atcttcccgc gcatgacggg ctctgggagg
     3781 cagcgcaggc gacagcgcat gacctgctgg cgcggctcgc ggtcgtgccg ctggtgctgg
     3841 aagcgcgcgg cctcgacgtg acgcccggca tgatcgagcg gctccgccgc ttcggcgacg
     3901 acgaaagcgc cgccgtgctg caggtgatct atgacgacga gatcggccat gtcgccgccg
     3961 gccggcgctg gttcgagtgg ttgtgccgtc gccgcggcgt gccgcccgct gcgacctatc
     4021 aagcgctcgt tcgcacgcat ttcaaaggcg ccctcaagcc acccttcaac cgcgccggcc
     4081 gcgacgccgc agagcttccc gccgccttct acgagccgct cgcgacgtga gacggctcac
     4141 agccgtactg ccgctcttcg gctaggctcc gatatgcagt cctggtgccg gttttgggca
     4201 agtcgttgtg atcgaaacac gcgattctat gtgtgcttga cacggcttga gcgggggtgg
     4261 ttgatggttg caggggttgc ggcggtgccg gcaatagaag tcttggggaa acagcatgga
     4321 tgacgggaaa cacgcgggcc accgcgctct ggccgatctt ttcgcatatc ctttcatgtc
     4381 gtgcctgatc gagcgtcgga cgcggcgcat cgcccgcggc acctcgatcg acgcgggccc
     4441 gctcagctat cagagcagca acaagcccgc accgctgacc aagctcgaag aggcgattct
     4501 catcgtctcg accggcgtca ccggattgac cacccatgac ggcccgctcg ttcgccccag
     4561 cgacaacggc aaggagctgg gcacgccgtt cctcaacatc accgcgcgca cggcaagcag
     4621 cgccgatgat tgtcaggcga caagcttctt cgtcatcaac gacgacggca tcttcctgct
     4681 gaagcagccg aaaggccgcg acgctctcca ggcgctcaag gacctgccgc ccaaatggtc
     4741 cgactggaag gagaaggact ggatcgacgc cgccgaatcc gtgaaagtgc gcgtctccaa
     4801 ccggcgcctc gatttcccgc gcgactttcc ttattacctc ggctggaatc gccagatttc
     4861 caacgcgccc ggctcgacgt tgttcttccc ggtcgtcgac tgcacctggc aatacatcaa
     4921 cgccatcctg atcctgctgt cggagcccga cggcaagcgt cccctcgtca tcgacgattt
     4981 ccgcaaattc catcccaagg ggctgatcga gatcgccgcc tggctcggct cgaaactggg
     5041 catcaccgag gagattccct atcagcccat cggcggcctc aaatgggtgc ggaacggttt
     5101 cgtcaacaaa gacaacccgg gaccgctcgg cctcacgcgc acgttgcgca cggactacga
     5161 atccttcttc gcgctgcaga atctgatgct gctgggacag gcgctgggcg tcggcggctg
     5221 gatccacagc tcggtgtttc cgccttacat ctacgagacc gatccgagca agggctggta
     5281 cggcctcgga ttccgcatgg tgacgccgaa gaagctgagc cccatgccgc cgatcccggc
     5341 gtcgcagccc aatcccgtcg gcatcgacgg catcctcgaa ggcctctgcc cgccttacgt
     5401 caagtcgatg gacgaggccg tcgatcgcgt gatcgaggcc aaatacggcg cgcgcggcag
     5461 ctatggcgac gccgacatgt tctcgcgctc ctatcgcgat cccaagatgg gcgcggagta
     5521 tctccgccag gcctcgcatt tctccgaccg cgcgatcggc tacacgaaag agatctgcgc
     5581 ctatctctgg gagacctatg gccgcttccc ggcgcatgtc gacgccttcc acacgccggg
     5641 catgtgggtg cagttctcgc atctcgagat ggagtattac gaccgcttct tcgatccgcg
     5701 gcaattcacg cgccaggcgc agcacgatgc gctctggcac cgctgacgcg gcggaccgtc
     5761 gggggacgca accatgctca gggcattgca gatcggcgcc aatatcgtcg ccgcgctctt
     5821 cctcgcagcg gcggtgggcc ttgtcctgat cgagcgaagc tgggtcgacc ccgagccggc
     5881 gaacccgcgc gatttcttcc tccagggctc gaccggcacc gagatcatgc cgctcgccgt
     5941 cttccaggtc ctgccgacgc ttttccccga ggagttccag cccgcgggcc cggcggcggg
     6001 cgactgggtc gatcagttcg gctttttgcg cggcgacccc aacgtcaacg aggggctgcc
     6061 ttacggcatc aacatcagcc attatcggcc gaaatctggg gcaccttcgc ccatcgcctt
     6121 cgtcggcttc aattgtgcga tctgtcacag cgcgaaggtc cataccggcg acgacaacgg
     6181 gcccgtcatc ctcggcatgg gcaattcggc catcgacctc gtcggcttcg gcgatgccgt
     6241 caagagttcg ctgctcgacg agacgcggct cacggtctcg agcatcgcct cgacctatga
     6301 gacggtgacg cacaagaagc tcggcctcgt cgacaagctc gtcatcgacg tctggctgag
     6361 cggcgcacgc accgccatcc gcagcgactt tcccatgcgc ggcacgccct tcagcgggcc
     6421 cgatgtcaga agcgccgcct tgatgggaag cggcccggcg cgcaacgagc cgatcaagga
     6481 gacggtgcgc ttcctcatcg acgagacgcc ggcgcccgac ggcggctact cgaagatccc
     6541 ctgcctctat cgccaggacc ggcgcgaatg ggcgc
//
LOCUS       DASYNS010000488        12322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2151519, whole genome shotgun sequence.
ACCESSION   DASYNS010000488 DASYNS010000000
VERSION     DASYNS010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 12322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12322
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2151519"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(549..640)
                     /locus_tag="VGP50_15195"
     tRNA            complement(549..640)
                     /locus_tag="VGP50_15195"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(604..606),aa:Ser,seq:cga)
     gene            832..1140
                     /gene="rplU"
                     /locus_tag="VGP50_15200"
     CDS             832..1140
                     /gene="rplU"
                     /locus_tag="VGP50_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV8028778.1"
                     /translation="MFAVIRTGGKQYKVAKDDVITVEKLAGEAGSTVTLEDVLMVAEG
                     GDAKLGAGGASVTAEVVEQIRSDKVLIFKKRRRHNYRRKNGHRQHLTVLRVTGIEAGK
                     "
     gene            1154..1429
                     /gene="rpmA"
                     /locus_tag="VGP50_15205"
     CDS             1154..1429
                     /gene="rpmA"
                     /locus_tag="VGP50_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV8028779.1"
                     /translation="MAHKKAGGSSRNGRDSRGRRLGVKKFGGETVVPGNIIIRQRGTK
                     YHPGRNTDMGRDHTIFSKIAGAVSYHTTTKGRTFVSVAPLPLEAAAE"
     gene            1521..2555
                     /gene="obgE"
                     /locus_tag="VGP50_15210"
     CDS             1521..2555
                     /gene="obgE"
                     /locus_tag="VGP50_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010667130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV8028780.1"
                     /translation="MKFLDQAKIFVKSGDGGAGAVAFRREKYIEFGGPDGGNGGRGGD
                     VIVEVVANLNTLIDYRYQQHFRAEKGHHGMGANRSGAAGAAVVMRVPVGTQILDAEND
                     QVLLDLTKPGERHVLAKGGDGGFGNAHYKSSTNRAPRRAGPGWPGEERWVWLRLKLIA
                     DAGLVGLPNAGKSTLLAAVSRAKPKIADYPFTTLKPQLGVVRVHDDEFVLADLPGLIE
                     GASEGAGLGHRFLGHVERCAVILHLIDGTAEDVVAHWRSIRRELGAYGHGLTEKPEII
                     GLNKADAVPPEDIAKMRRKLQRASKRPVMVLSGATGAGVPEAMAALLDVIKQTRADAE
                     AAKTEEAPAS"
     gene            2552..3667
                     /gene="proB"
                     /locus_tag="VGP50_15215"
     CDS             2552..3667
                     /gene="proB"
                     /locus_tag="VGP50_15215"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV8028781.1"
                     /translation="MSGNLAAARRLIVKIGSALLVDDATGDIRRPWLAALVEDIARCR
                     ARHQEMLIVSSGAIAVGRRHLGLSGRSLKLEEKQAAAATGQIRLAHAYQEALAKHGLT
                     VAQILLTPEDTEERRRHLNARATLEQLLALGAVPVINENDTVATAEIRFGDNDRLAAR
                     VAQMISADTLVLLSDIDGLYTADPRKAPDARHLAEVREITPEIEAMGGEAATGYSSGG
                     MVTKLAAARIALAAGCRMVIARGDQLHPLSAIEAGARATWFIPAAEPRTARKRWIAGA
                     LNPMGAFVVDAGAAAALKRGTSLLPAGVVAIEGSFERGDAIVVKSAEGRELARGLTAY
                     SSSDARVIAGHKSGEIEALLGYRGRDEMIHRDDLVVS"
     gene            3855..5120
                     /locus_tag="VGP50_15220"
     CDS             3855..5120
                     /locus_tag="VGP50_15220"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV8028782.1"
                     /translation="MTTASDIESQMRTIGGAARHAAALLARAPAERKTAALRAAASVI
                     RDARAAILEANARDLVEAGNDLSAAMRDRLVLDEKRVEGMARGLEDVAALPDPVGAVL
                     ARWTRPNGLEISRVSIPLGVIGIIYESRPNVTADAGGLSLRAGNAAILRGGSESFHSS
                     RAIADTLRRGIASAGLPEDAVQLVPTRDRAAVGAMLGMTEFIDIIVPRGGRSLIERVQ
                     RESRLPVIAHLEGNCHVYIDGAADFAKARRIVLNAKMRRTGVCGAAETLLVDRRVAEL
                     YLRPILADLHQAGCEIRGDAAVRALDPQAKTATDADWATEYLDAILAVRVVDGIGEAI
                     AHVNRWGSHHTDAIVTEDQQAAERFLAEVDSAIVLENASTQFADGGEFGMGAEIGIST
                     GRLPPRGPVGAEQLTSYKYVVRGNGQVRP"
     gene            5135..5752
                     /locus_tag="VGP50_15225"
     CDS             5135..5752
                     /locus_tag="VGP50_15225"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV8028783.1"
                     /translation="MLGNLAPPRIGLLGGSFNPAHAGHLHISLEALKRLGLDEVWWLV
                     APQNPLKPVRGMASLEKRLAAARRIGAHPRIRVFDLETPLGTRYTADTVKELQRIFPR
                     RRFVWLMGADNLAQIRHWKHWNEIFSRVPIAVFARPTYCLKGLAELAAKRYARRRVAP
                     EAARRLADLVPPAWVFLPTRLDASSATEIRSHRAPKRARRERRKS"
     gene            5759..6154
                     /gene="rsfS"
                     /locus_tag="VGP50_15230"
     CDS             5759..6154
                     /gene="rsfS"
                     /locus_tag="VGP50_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV8028784.1"
                     /translation="MTAIPRRKSQPQPDAAIPSTLLGLTEKALADGKAEDLVVIDLQG
                     KSDIADHMVIATGRSQRQVLALADRVLQALRDHGHGRAAVEGLPHGDWVLIDAGDVII
                     HLFRPEVRTYYNLEKMWDATLAEPVTVLA"
     gene            6176..6634
                     /gene="rlmH"
                     /locus_tag="VGP50_15235"
     CDS             6176..6634
                     /gene="rlmH"
                     /locus_tag="VGP50_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV8028785.1"
                     /translation="MRLHVIAVGRPKGGPHEALVRYYAERITWPLSLRDVEEKRPLSA
                     NEIKEREGALLLAAAPARATLVALDARGQALTSEGLAERVQRWRDGGIGDLAFLIGGA
                     EGLADTVREKAHFLLSLGVMTWPHLLARAMLLEQLYRAQQILAGHPYHRA"
     gene            6740..8659
                     /locus_tag="VGP50_15240"
     CDS             6740..8659
                     /locus_tag="VGP50_15240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028786.1"
                     /translation="MTSVTLEGIAWVDYAPAPALDAAQWVEHAPVPALAIDADGRITA
                     ANDAMARLLGSEIAQLLGKELALWSSDPPALRNFLGTTVSTTAELRFRASDGRDRCFL
                     LSIAPSTLAGGRMVSVVDVTAKRADERQLRDDIGRLREMIAAGSGWFHELDRSQTQVR
                     VFRRDVVNNRPALMDMTVRFPGEVIDPAFDPKGIAKAMLDYAEQKPVRNFLHRLPRSD
                     GSEIYMLANSVPFYDENGVYQGHRGVSVDVTAQVLAERALRRAQQHLERAQRVAAIGS
                     IDRDLVTGAVDWSEEMYRLLGIDRSFERTEASIPSLAHPEDRARVVADIARLRSGVLA
                     PPDEYRMIRPDGETRIIRVESDFQYDTAGKPVRLLSIFKDVTELRTAERRQREQLQHS
                     QKLEALGTLAGGVAHDLNNTLVPILALTKIVAKRLPEDSRERKSLGIVVQAGERARDL
                     VKQILAFSRKEAPTRKSFDLGALVGEALTLLRASVPSTISIVENIAAAPPLLGDPGQL
                     HQVVTNLVTNAAHAIADKIGTITVELAAAPGETITEVTKRACGTAIRLSISDTGCGMD
                     EATLARMFEPFFTTKGVGEGTGLGLSMVQGIVGQHGGCVKVTSRKGEGTRFDLYLPAL
                     DAEAEALLGDDAHAA"
     gene            8746..10308
                     /gene="gpmI"
                     /locus_tag="VGP50_15245"
     CDS             8746..10308
                     /gene="gpmI"
                     /locus_tag="VGP50_15245"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEV8028787.1"
                     /translation="MPKATRPRPVVLCVLDGWGCRAERADNSILLAQTPNWTRFLKDS
                     PHAQLQASELFVGLPKGQMGNSEVGHTNLGAGRVVLQDLPRIDQAIADGSLSKSPALA
                     AFIAALKKSGGAAHLFGLLSPGGVHSHQDHIAALVRLLAEAGLAVTVHAFLDGRDTPP
                     KSAGPYLAKFADDVAGLGRVRIATVSGRYYAMDRDKRWDRVAKAYAALVQGQGEHAGD
                     AQAAVRQAYAQGQNDEFVLPTVIGDYSGMADGDGVLCANFRADRVREILAALLDPDFA
                     GFPRERRPRFAAALGMTEYAVELNRFLATIFPPEDLGDTFGEVVSQAGLTQLRIAETE
                     KYAHVTFFFNGGREAEFPGESRILVPSPKVATYDLKPEMSAPEMTDKLVEAIDHGRFD
                     VVVVNYANTDMVGHSGDEAAAIKAVEAVDRCLGRLADAVRRAGGSLLITADHGNAEMM
                     RDADTGQPHTAHTLNPVPVVLVNPPAGITGLSNGRLADVAPTLLALLGLKQPAAMTGR
                     PLLLRAAERARA"
     gene            10295..11599
                     /locus_tag="VGP50_15250"
     CDS             10295..11599
                     /locus_tag="VGP50_15250"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEV8028788.1"
                     /translation="MRGPEPSFPRLIALLFAVAFPCLSAQGADAPEQRLKSVEKQIEE
                     SRAKEAQSARAADALAAEIAALRSDSIAAARAAQEQEGALSALDEQLAALGAEEARKA
                     AELMRHRAQQTQLLMALERLAQHPPEALALAPEDPLDAVRSARLMGAAVPPLEKAARA
                     LRQEIVALGSLRQQIADAQVSRRGKGLALKEEQTRLAALIERKTALQRQAQHEVEESG
                     QRQAQLATQASDLQQLIERLSAERQVREAEAQRREEEESRREEQRLATLAEPPTAPSD
                     NATVEVAAPAPLRLDPSKPRQLRPFAGARGHMIYPASGHLMLRYGENDEFGVASKGLT
                     LETRAGAQVIAPFDGRVLFAGPFKGYGQILIIEHGDGYHSLLAGLDRVEGVVGQWVVA
                     GEPVGLMAGGDTKPHLYLELRHDGQPINPLPWLATRDEKVSG"
     gene            11599..>12322
                     /locus_tag="VGP50_15255"
     CDS             11599..>12322
                     /locus_tag="VGP50_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV8028789.1"
                     /translation="MRLALTAASAATVVWLLAPPVVAAEQTPPTPSNSDTYRQLDLFG
                     EVFERVRADYVEEVSDEQLIEAAINGMLSALDPHSSYMNAKNFRDMQVQVRGEFGGLG
                     IEVTMENGLVKVVSPIDDTPAARGGLKPGDFITHIDGEPVAGLSLNEAVERMRGPVNS
                     DIKLTIRREARDPFDVKLTRAVVKIQSVKWHLEGADVGYIRITSFNEQTDSGLQSAMK
                     DLRSKAADRLTGIILDLRNNPGG"
BASE COUNT         2083 a         4271 c         4136 g         1832 t
ORIGIN      
        1 ccgcgtcctc ggcggcctcg agcgcacgct ggccgcgccc gacacgacag ccgccttcat
       61 cgccgaatac gaggccgagc gaaggcgcct cctgggccac gccgccgacg cccgagcgcc
      121 cctcgagcgc cggcgcaccg cgctcgagaa gcgcttgaag catctcgtcg agaccgttgc
      181 cgagggcctc tcctcgcccg cggtgaacgc cgcgatcgtc gcggccgaga cggagagcgc
      241 ggcgatcgcc gccgagctcg cggcgctgac gccggcgccg gcgatcgaca tcctcggcgt
      301 gaccgccgcc gcttatcgcc gcacgctcgc ccgcctcgcg gccgcgctcg ccggcggcga
      361 cgagcagctc cgccaggacg cgcggcgcct cttgcgcggc gtcgtcgccg cgatcgagat
      421 cgcgccggca gcggatggcg gcgtcacggt caccgtgaag ggcgtgatcg aaggcctcct
      481 cgctctcgtc gacacgcgga cgcgtggcgg cggcgggaac gaaggcggca caaaccggac
      541 tgtgaagctg gcggagaggg agggattcga accctcggta ccccgcaagg ggcacaacgg
      601 ttttcgagac cgccccgatc gaccactctg gcacctctcc gcaagaccga ggaacctgcc
      661 catcgagggg cgcggagact accccaggat ctcgcgacct cgcaaccgcc gcggccaggg
      721 tctgcgacct gaatcttgac aggagaggcc cgcccgctat agtgcgctcc ctgcggacgg
      781 gacagccccg tccgttcgag tttagagacg cgagaacggc ccgggtccct catgttcgca
      841 gtcatccgca ccggcggcaa gcagtataag gtcgccaagg acgacgtcat caccgtcgag
      901 aagctcgcgg gcgaggccgg ctcgaccgtg acgcttgagg acgtcctcat ggtcgccgag
      961 ggcggcgacg ccaagctggg cgccggcggc gcctcggtca ccgccgaagt ggtcgagcag
     1021 atccgcagcg acaaggtgct gatcttcaag aagaggcggc gccacaacta ccgccgcaag
     1081 aacggccacc gccagcatct gaccgtgctg cgcgtcaccg gcatcgaggc cgggaagtaa
     1141 aggagagaag gccatggcgc ataagaaagc aggcggctcc tcgcgcaacg gtcgcgattc
     1201 ccggggacgg cgcctcggcg tcaagaagtt cggcggcgaa acggtggtgc ccggcaacat
     1261 catcattcgc cagcgcggca ccaagtacca ccccggccgc aacaccgata tgggccgcga
     1321 tcatacgatc ttctccaaga tcgccggcgc cgtgagctat cacacgacca cgaagggccg
     1381 caccttcgtc tccgtcgccc cgctgccgct ggaggccgcc gccgaatagg ccgttccgcc
     1441 cggcgcccga ggcgccagca cagaagatgc tcgaggggga atggtcgcca ttcccccttt
     1501 ttcgttggca cagctgaccc atgaaatttc tcgaccaggc caagatcttc gtgaagagcg
     1561 gcgacggcgg tgccggggcg gtcgctttcc ggcgcgagaa atacatcgag ttcggcggcc
     1621 ccgacggggg caatggcggg cgcggcggcg acgtcatcgt cgaagtcgta gcgaacctca
     1681 acacgctcat cgactaccgc taccagcagc atttccgcgc cgagaagggc catcacggca
     1741 tgggcgccaa ccgcagcggc gccgccggcg ccgccgtggt gatgcgcgtc ccggtcggca
     1801 cgcagatcct cgacgccgag aacgatcagg tgctgctcga tctgacgaag cccggcgagc
     1861 gccatgtgct ggcgaaaggc ggcgatggcg gtttcggcaa cgcccactac aagagctcga
     1921 ccaaccgggc gccccgccgc gccggtccgg gctggcccgg cgaggagcgc tgggtgtggc
     1981 tgcgcctcaa gctcattgcc gatgccgggc tcgtcgggtt gcccaatgcc ggcaagtcga
     2041 cgctgctcgc tgcggtgtcg cgcgccaagc cgaagatcgc ggactatccc ttcacgaccc
     2101 tgaagccgca gctcggcgtc gtgcgcgtcc atgacgacga gttcgtcctc gcggatctgc
     2161 ccggcctcat cgaaggcgct tccgaaggcg cgggcctcgg ccatcgcttc ctcggccatg
     2221 tcgagcgctg cgcggtgatc ctgcatctga tcgacggcac ggccgaggac gtggtggcgc
     2281 attggcgcag catccgccgc gagctcgggg cttatggcca tgggcttacc gagaagcccg
     2341 agatcatcgg cctcaacaag gccgatgcag tgccgcccga ggacatcgcg aagatgcgcc
     2401 gcaagctgca gcgcgcgagc aagcgtccgg tgatggtgct gtcgggcgca acgggcgccg
     2461 gcgtgccgga agcgatggca gcacttctcg acgtgatcaa gcagacgcgc gcggacgccg
     2521 aagccgccaa gacggaggag gcgccggcgt catgagcggc aatctcgccg ccgcgcgacg
     2581 gctcatcgtc aagatcggct cggcgctcct ggtcgacgat gccacgggcg acatccgccg
     2641 gccctggctc gcggcgctcg tcgaggacat cgcccgttgc cgcgcgcgcc accaggaaat
     2701 gctcatcgtc tcctcgggcg cgattgcggt cggccgccgc catctcggct tgtcgggccg
     2761 ctcgctcaag ctcgaggaga agcaggcggc ggcagcgacc ggacagatcc gcttggcgca
     2821 tgcctatcag gaggcactgg cgaagcatgg gctgaccgtg gcgcagatcc tgctcacgcc
     2881 cgaagacacc gaggagcggc gccgtcatct gaacgcgcgg gcgacgctcg agcagctgct
     2941 ggcgctgggc gcggtgccgg tgatcaacga gaacgacacg gtggcgacgg ccgagatacg
     3001 cttcggcgac aatgaccggc ttgccgcgcg cgtggcccag atgatcagcg ccgatacgct
     3061 ggtgctgctc tccgatatcg acggacttta caccgccgat ccccgcaaag cgccggatgc
     3121 gcggcatctc gcggaagtgc gcgagatcac gcccgagatc gaagcgatgg gcggcgaggc
     3181 agcgacgggt tacagctccg gcggcatggt gacgaagctg gcggcggcac gcatcgccct
     3241 ggccgcgggc tgccgcatgg tgatcgcgcg gggcgaccag ctgcacccgc tcagcgccat
     3301 cgaggcgggc gcgcgcgcca cctggttcat tccggcggcc gagccgcgca ccgcccggaa
     3361 gcgctggatc gccggcgcgc tcaatcccat gggcgccttc gtcgtggatg ccggtgcggc
     3421 cgccgccttg aagcgcggca cgagcttgct cccggccggt gtggtcgcga tcgagggcag
     3481 cttcgagcgg ggcgacgcga tcgtggtgaa aagcgccgaa ggacgcgagc tggcgcgcgg
     3541 gctcaccgcc tattccagca gcgacgcgcg ggtcatcgca ggccacaaaa gcggtgaaat
     3601 cgaggccctc ctcggctacc gtggacgcga cgagatgatc catcgcgacg atctggtggt
     3661 gagctagatg cgccgggttg tgccgtatgc gccgccgtca ctctccctct ccgccctcgg
     3721 gggcggagag ggttggggtg aggtgggggc atttcggcac caacctgtcc gccatcgaca
     3781 cccacctcat cctaaatcct tctccgccct gaagggcgga gaaggacttg aacctcgcgg
     3841 tcggtactag agcaatgaca acggcttccg acatcgaatc ccaaatgcgt acgatcggcg
     3901 gcgctgcgcg gcacgcagcg gcgctgctcg cgcgcgcccc ggccgagcgg aaaaccgctg
     3961 cgcttcgggc agcggcgtcc gtgatccgcg atgcgcgcgc agccattctc gaggccaatg
     4021 cgcgcgactt ggtcgaggcc ggcaacgacc tctcggcggc gatgcgcgat cgcctggtgc
     4081 tcgacgagaa gcgcgtcgag gggatggcgc gtggtctcga agacgtcgcg gcgctccctg
     4141 atcccgtggg cgcggtgctg gcgcgctgga cgcggcccaa cggcctcgag atctcgcgcg
     4201 tcagcatccc gctgggggtc atcggcatca tctatgaatc gcggcccaat gtgacggcgg
     4261 atgccggcgg gctctcgctg cgcgccggca atgcggcgat cctgcgcggc ggctccgaga
     4321 gcttccactc gtcgcgcgcc atcgccgaca cactgcgccg cggcatcgcg tccgcgggtc
     4381 tcccggaaga tgccgtgcag ctcgtgccga cgcgcgaccg cgcggccgtc ggcgcgatgc
     4441 tcggcatgac cgaattcatc gacatcatcg tgccacgcgg cggccgctcg ctcatcgagc
     4501 gggtgcagcg cgagagccgc ctgccggtca tcgctcatct cgagggcaat tgccacgtct
     4561 atatcgacgg cgccgccgat ttcgccaagg cgcgccgcat cgtgctcaat gccaagatgc
     4621 gccgcaccgg cgtgtgcggc gccgccgaaa cgctgctggt cgaccgccgc gtggcggagc
     4681 tctacctgcg gccgatcctc gccgatctgc atcaggcggg ttgcgagatc cgcggcgatg
     4741 ccgccgtgcg cgcgctcgat ccccaggcga aaacggcaac ggatgccgac tgggccaccg
     4801 aatacctcga cgccatcctc gccgtgcgcg tcgtcgacgg tatcggcgaa gcgatcgccc
     4861 atgtgaaccg ctggggctcg catcacaccg atgctatcgt gaccgaagac cagcaggcgg
     4921 ccgagcgttt tctcgccgag gtcgacagcg ccatcgtcct cgagaacgcc tcgacgcaat
     4981 tcgccgatgg cggcgagttt ggcatgggcg ctgagatcgg catctccacg ggtcgcctgc
     5041 cgccacgcgg ccccgtcggc gccgagcagc tcaccagcta caaatacgtc gtgcgcggca
     5101 acgggcaggt gcgcccctga gtctgccccg gccgatgctc ggcaacctcg ccccgccgcg
     5161 gatcggcctt ctcggcggct cgttcaatcc cgcccatgcg gggcatcttc atataagtct
     5221 cgaagcgttg aagcggctcg gcctcgacga agtgtggtgg cttgttgcac cgcaaaatcc
     5281 gctgaaaccg gtgcggggca tggcctccct cgagaagcgc ctggcggcgg cgcggcgcat
     5341 cggcgctcat ccgcggatcc gggtcttcga tctcgagaca ccgctcggca cccgatacac
     5401 cgcggataca gtaaaggaat tgcaacgaat cttcccgcgg cggcgtttcg tatggttgat
     5461 gggtgctgac aacctggcac aaatccgcca ttggaagcat tggaacgaga tcttctcgcg
     5521 tgttcccatt gcagtcttcg cccgccctac ctattgtctt aaaggactgg ccgagctggc
     5581 agcgaaacgg tatgcccgcc ggcgggtggc gcccgaggcg gctcgacgcc tcgccgatct
     5641 ggtccctccc gcctgggtgt tcctgcccac aaggctcgat gcgagctcgg caacggagat
     5701 ccgctctcat cgcgcgccca agcgcgcgag gcgggagcgg cggaagtcgt gacaggaggt
     5761 gaccgccata ccgcgccgta aatcgcagcc gcagcccgat gcggctattc catcgaccct
     5821 tctcggtctc accgagaagg ctctcgccga cggcaaggcc gaagatcttg tcgtcatcga
     5881 tctccaaggc aaatccgaca tcgccgatca catggtcatc gccaccgggc gctcgcaacg
     5941 ccaggtgctg gcattggccg atcgcgtgct gcaagccctt agagaccacg gccatggtcg
     6001 cgccgcggtg gaagggttgc cccatggcga ctgggtgctg atcgatgccg gcgatgtcat
     6061 catccatctg ttccgtcccg aggtgcgcac ctactacaac ctcgagaaga tgtgggacgc
     6121 gacgctggcc gagcccgtta ccgtgctcgc ctgagcgttt ctcgaggcgc ggcacatgcg
     6181 ccttcacgtg atcgccgtcg gccgccccaa gggcggaccg catgaggcgc tcgtgcgata
     6241 ttatgccgag cgaatcacct ggccgctcag cctccgcgac gtcgaggaga aacgtccgct
     6301 ttccgcgaac gagatcaagg agcgcgaagg cgcgttgctg ctcgcggcgg cgccggcgcg
     6361 agccaccctc gtggcgctcg atgcgcgcgg ccaggcgctg acgagcgaag gcctcgccga
     6421 gcgcgtacag cgctggcgag acggcggcat cggcgatctc gcttttctca tcggcggtgc
     6481 cgaggggctc gccgacacgg ttcgcgagaa ggcgcatttc ctgttgtcgc ttggcgtcat
     6541 gacttggccc catctcctcg cccgggccat gttgctcgag cagctctacc gcgcgcagca
     6601 gattctcgcc ggccatccct atcatcgcgc ctgagccatg cgccgtttcc cggcctagat
     6661 cgatcggcta tcccggcgcg taacccttgc taaaggccgc actgctaaga ctcgaacgag
     6721 ccatggccgg gagaactccg tgacgtccgt caccttggaa ggaatagcct gggtcgacta
     6781 tgcgccggca ccggctctgg atgcggccca gtgggtcgaa catgcgcccg tacccgctct
     6841 ggctatcgac gcggatggcc ggatcacggc cgcaaacgac gccatggcgc ggctccttgg
     6901 ctcggagatc gcgcagctcc tgggaaaaga gctcgcgctc tggagcagcg atccgccagc
     6961 gttgcgcaac ttcctcggaa ccacggtgag cacgaccgca gagctccgct tccgcgcatc
     7021 cgacgggagg gaccgctgtt tcctgctgtc gattgcgccg agcacgcttg cgggtgggcg
     7081 catggtgagc gtggtcgacg tgacggccaa gcgcgccgat gaacggcagc tgcgcgacga
     7141 catcggccgg ctgcgggaga tgatcgccgc gggatcgggc tggttccacg agctcgatcg
     7201 cagccaaacg caggtgcggg tgttccgtcg cgacgtggtg aacaaccgtc ccgcgctcat
     7261 ggacatgacc gtcagatttc ccggagaagt catcgacccc gccttcgacc ccaaaggcat
     7321 cgccaaagcg atgctggatt acgccgagca gaagccggtg cgtaacttcc ttcatcgtct
     7381 gccgcgtagc gacggcagcg aaatctacat gctggccaac agtgtgccct tctatgacga
     7441 gaacggcgtc taccagggcc accgcggcgt cagcgtcgac gtgacagcgc aggtgctcgc
     7501 cgagcgcgcc ttgcgccgtg cgcagcagca tctcgaacgg gcgcagcgcg tggcggcgat
     7561 cggcagcatc gaccgcgacc tcgtcacggg agcggtcgac tggtcggaag agatgtaccg
     7621 tcttctcggg atcgaccggt ccttcgagcg caccgaagca agcatcccct cgctcgcgca
     7681 tcctgaagat cgcgcccgtg tcgtggccga catcgcgcgg ctgcgcagcg gcgtgctggc
     7741 gccgccggac gaatatcgga tgattcgtcc cgacggcgag acccggatca tccgtgtcga
     7801 gagcgatttc caatatgaca ccgccggcaa gccggtccgg ctcttatcga tcttcaagga
     7861 cgtgacggag ctgcgcacgg cagaacgccg gcaacgcgag cagcttcagc attcacagaa
     7921 gctcgaagcg ctcggcacgc ttgccggggg cgtcgcccat gatctcaaca acacgctggt
     7981 ccccattctc gccttgacca agatcgtcgc caagcgcctg cccgaggaca gccgcgagag
     8041 gaagagcctc ggcatcgtcg tccaggcggg cgagcgcgcg cgcgacctcg tgaagcagat
     8101 cctcgccttc agccgcaagg aagcgccgac gcgcaagagc ttcgatctcg gcgcgcttgt
     8161 cggcgaggcg ctgacgctgc tacgcgccag cgtaccctcc accatctcca tcgtggagaa
     8221 catcgccgcg gcaccgccac tgctcggcga ccccggacag ctgcaccagg tggtcaccaa
     8281 tctcgtcacc aatgccgccc acgccatcgc cgacaagatc ggaaccatta ccgtcgagct
     8341 ggcggcggcg ccgggcgaga cgattaccga ggtcaccaag cgggcttgcg gcaccgccat
     8401 tcgcctttcg atcagcgata ccggttgcgg catggacgag gccacgctcg cgcgaatgtt
     8461 cgagccgttc ttcaccacca agggcgtcgg cgagggcacg ggcctcgggc tctcgatggt
     8521 gcaaggcatc gtcggccagc atgggggctg cgtcaaagtg accagccgaa agggcgaggg
     8581 aacgcgcttc gacctttatc tgccggcgct cgacgcggaa gccgaggcgc ttcttggcga
     8641 cgacgcgcac gccgcctgaa ttccgttagt ggcgcgttaa ccacatcgac ctaaggaaag
     8701 gtggagcact acccttcatt cggtcgttcc tctataagca tgaccatgcc gaaagcgacc
     8761 cgcccccgcc cggtcgtgtt atgcgtcctc gacggttggg gttgtcgcgc ggagcgcgcg
     8821 gacaacagca tcctgctggc gcagacgccc aactggaccc gcttcctcaa ggactcgccc
     8881 catgctcagc tgcaggcgag cgagctcttc gtcggcctgc cgaaagggca aatggggaat
     8941 tccgaggtcg gccacacgaa tctcggggcc ggccgcgtcg tcctccagga cctgccgcgc
     9001 atcgatcagg cgattgccga tggcagcctc tcgaagagcc cggcgcttgc cgcattcatc
     9061 gcggcgctca agaagagcgg cggcgcggcg catctgttcg ggttgctgtc gcccggcggc
     9121 gtccattcgc atcaggacca catcgcggcg ctggtgcggc tgctggccga agccgggctc
     9181 gccgtgacgg tccatgcgtt cctcgatggc cgcgacacgc cgcccaagag cgccgggccc
     9241 tatctcgcca aattcgccga cgacgtcgcc ggcctcgggc gcgtgcgcat cgcgacggtg
     9301 agcggcaggt actacgcgat ggaccgcgac aagcgctggg accgcgtggc gaaagcctat
     9361 gccgccctgg tccagggcca aggcgagcat gcgggcgacg cccaggccgc ggtccggcag
     9421 gcctatgcac agggccagaa cgacgaattc gtgctgccga cggtcatcgg cgattattcg
     9481 ggcatggccg acggcgacgg cgtgctttgc gccaatttcc gcgccgaccg cgtgcgcgag
     9541 atcctggcgg ctctgctgga tcccgacttc gccggttttc cgcgcgagcg ccggccgcgc
     9601 ttcgccgcgg cgctcggcat gaccgaatac gcggtcgagc tcaaccgctt tctcgcgacg
     9661 atctttccac ccgaagacct cggtgacacc ttcggggaag tggtgtcgca ggcgggcctg
     9721 acgcagctgc gcatcgccga gaccgagaaa tacgcgcatg tcacgttctt cttcaatggc
     9781 gggcgcgaag cagagtttcc cggcgagagc cgcatcctcg tgccctcgcc caaggtcgcc
     9841 acctatgacc tcaagcccga gatgtcggcg ccggagatga cggacaagct cgtcgaggcg
     9901 atcgatcacg gccgcttcga cgtcgtcgtc gtcaattacg ccaacaccga catggtcggt
     9961 catagcggcg atgaggcagc ggcgatcaag gcggtggaag cggtcgatcg ctgtctcggg
    10021 cgtctggccg acgcggtgcg gcgcgccggc ggctcgctcc tcatcacggc cgatcacggc
    10081 aacgccgaga tgatgcgcga cgccgacacg ggccagccgc acacggcgca cacgctcaac
    10141 cccgtgccgg tggtgctggt gaacccgccg gccggcatca cgggcctcag caacggccgg
    10201 ctggccgacg tcgcgccgac cctcctggcg ctcctcggcc tgaagcagcc cgccgccatg
    10261 accggccggc cgctgctgct gcgcgctgcc gagcgtgcgc gggcctgaac cctcctttcc
    10321 tcggctgatc gcactgctgt ttgcggtcgc cttcccgtgc ctttcggcgc agggcgccga
    10381 cgcccccgag cagcggctga aatcggtcga aaagcagatc gaggaaagcc gtgccaagga
    10441 ggcgcaatcc gctcgcgccg cagatgcgct ggcggccgag atcgcggcgc tgcgcagcga
    10501 tagcatcgcc gccgcccggg cggcgcagga gcaggagggg gcgctctccg ccctcgatga
    10561 gcagcttgcg gcgctgggcg cggaggaagc gcgcaaggcg gcggaactca tgcgtcatcg
    10621 cgcccagcag acgcagcttc tcatggcgct cgaaaggctg gcgcagcatc cacccgaagc
    10681 tctcgcgctg gcgcccgagg atccgttgga cgccgtgcgc agcgcccggc tcatgggcgc
    10741 ggcggtgccg cccctcgaga aggcggcgcg cgcgctgcgc caggagatcg tcgcgctcgg
    10801 cagcttgcgc cagcagatcg ccgacgccca ggtgagccgc cgcggcaagg gactggccct
    10861 caaggaggag cagacgcgcc ttgcggcgct catcgaacgc aagaccgcgc tgcagcgcca
    10921 ggcgcagcac gaggtcgagg agagcggcca gcgccaggcg cagctcgcca cccaagcctc
    10981 ggatctgcag cagctcatcg aacgcctctc ggccgagcgc caggtccgcg aggccgaggc
    11041 gcagcggcgc gaggaggaag agtcgcgccg cgaggagcag cggctggcga cactcgcgga
    11101 accgccgacc gcaccgtccg acaacgccac ggtcgaggtg gcggccccgg cgccgctccg
    11161 gctcgacccg agcaagcccc ggcagctccg ccccttcgcg ggcgcccgcg gccatatgat
    11221 ctacccggcc agcgggcacc tgatgctgcg ctatggcgaa aacgacgagt tcggcgtggc
    11281 gagcaagggt ctgaccctcg agacgcgggc cggcgcccag gtgatcgcgc cgttcgacgg
    11341 ccgcgtgctg ttcgccgggc cgttcaaggg ttacgggcaa atcttgatca tcgaacacgg
    11401 cgacggatat cattcgctcc tggcggggct ggaccgcgtc gaaggtgtcg tcggacaatg
    11461 ggtggtggcc ggcgagcccg tcggcctcat ggccggcggc gacacgaagc ctcacctcta
    11521 tctcgagctg cgccatgacg gccagccgat caacccgttg ccgtggttgg caactcgcga
    11581 tgaaaaggta agtggatgat gcgtcttgct ctaacggccg ccagcgccgc aacggtcgtc
    11641 tggctcctcg cgccgcccgt cgtcgccgca gaacagaccc cgccgacgcc atccaactcc
    11701 gacacttatc ggcagctgga cctgttcggc gaggtgttcg agcgggtgcg cgccgactac
    11761 gtcgaagaag tctcggacga gcagctcatc gaggccgcga tcaacggcat gctgagcgcc
    11821 ctcgacccgc attcgagcta catgaacgcc aagaacttcc gcgacatgca ggtgcaggtg
    11881 cgcggcgagt tcggcggcct cggcatcgag gtcaccatgg agaacggcct cgtcaaggtc
    11941 gtctcgccca tcgacgacac gccggcggca cggggcgggc tgaagcccgg cgacttcatc
    12001 acccatatcg acggcgagcc ggtcgccggc ttgagcctca acgaggccgt cgagcgcatg
    12061 cgcgggcccg tcaacagcga catcaagctc accatccgcc gcgaggcgcg cgatcccttc
    12121 gacgtcaagc tgacgcgtgc cgtggtgaag atccaatcgg tcaaatggca tctcgaaggc
    12181 gccgatgtcg gctacatccg catcaccagc ttcaacgagc agaccgacag cggcctgcag
    12241 agcgccatga aggacctgcg gagcaaggct gccgaccggc tcacgggaat catcctcgac
    12301 ctgcgcaaca atccgggcgg cc
//
LOCUS       DASYNS010000489         4309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_591924, whole genome shotgun sequence.
ACCESSION   DASYNS010000489 DASYNS010000000
VERSION     DASYNS010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591924"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VGP50_15260"
     CDS             <1..325
                     /locus_tag="VGP50_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028790.1"
                     /translation="CMARWYKYELGGEQSIEIQSEYFAGHLFVNGVERGDRVAIIEDT
                     ISTGGTLIGLIGAVKEAGATVASAIALVEKTDNCGVRRVHDATGVTVTTIMKIAVGKD
                     GVRIV"
     gene            484..2739
                     /locus_tag="VGP50_15265"
     CDS             484..2739
                     /locus_tag="VGP50_15265"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV8028791.1"
                     /translation="MPDGLRSNGPALTRRQFGLAAGALTIAFSFAPAAAEPAKLPGSL
                     DHNRMLDAWLRIEASGAVTVCTGKVELGQGILTALEQIVADELDVAPTRIRIISGDTA
                     QTPDEGFTSGSQSIEYSGTALRYACAEARSILVSLALKRFHLDGKIADSEMAVVDGTI
                     TVGPQKRKITYWQLAADAQRELHREATAAISPKPAEAHKLIGTSRPRRDIPAKVMGAA
                     SYIQDMRLPGTLFGRVVRPPSYTAELESVDTARVEKLPGVVAVVRDGRFLGVLAKREE
                     QAIAAREALRSAAVWRSGTPLPPSDAILDAIQAFRNDVREISEKGEGQDLPNQANWRS
                     FVARYSRPFIAHASIGPSCAIAQMSEGRLTVWTHSQGVFPLRRDLAKALDLDESSIRA
                     IHVQGSGCYGHNGADDAALDAALLARAAQGRPVKLQWMRDDEFAWEPFGPAMVASVGA
                     RLDEQGKIVAWAYDVHSNTHSTRPGQQDGCNLLAAWHLATPKAPSPPEMIPQPAGGGD
                     RNAIPLYDFPWQKVLHHFIRPMPIRVSALRALGAHCNIFAIESFMDELALAAGVDPVE
                     FRLHHLSDPRGRAVIETAAKEAGWTLGAAGGNGQGRGLGFAKYKNLSGYVAVVAEVTV
                     DRDSGAVRVTKATAAIDAGQVVNPDGLVNQTEGGIVQATSWTLKEEVAFDAAHITSRD
                     WSTYPILTFPEVPEIKVTVIDRPGERSLGAGEAAQGPTAAAIANAVAHATGARLRDLP
                     LLPERVKAALG"
     gene            2926..>4309
                     /locus_tag="VGP50_15270"
     CDS             2926..>4309
                     /locus_tag="VGP50_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028792.1"
                     /translation="MDDGRGARAEATAPRGGIPLALAPLLSPYRRHGQLSVRVERLPN
                     GARFSKGQNNGDRTWSLRPDELDDVLYQPPEGLEETHTLAVRILSLDGDQGSTLAVLD
                     LPVSPADARAIVPPPMTAPGAPAGNAPVHNDGELRRLRDQLTELTARLAVREAELAEA
                     RQKFELAQGAGSKQAIEGQLAAARVLWEAELKDRLAAAAAEATTTLATSRDSWQAEQG
                     GRFAELEKRADEQIQLAQHRWQQDAKAAMSHAEASWKSGEAARLAAAEARLREEWTRA
                     LAEAEARVQRAETALAQLRPEEQIQRARDAWQQEARTALARAEESWKANEAARHAASE
                     ARWREEYRRSLAETEIRAERAEAALAQARQENAALPLPEKSAKTDEAVRLATTETRRR
                     EEAERALAETRLRAERTEAALAQAQTRSSGDAIEIHVLHDELRDVKAALALRETQLAD
                     ARSAVERRGAS"
BASE COUNT          762 a         1458 c         1493 g          596 t
ORIGIN      
        1 gtgcatggcg cgctggtaca aatacgagct cggcggcgag caatcgatcg aaatccaaag
       61 cgaatatttc gccggccacc tctttgtgaa cggcgtcgaa cgtggcgacc gggtcgcgat
      121 catcgaggat acgatcagca ccggcggcac tttgatcggt ttgatcggcg cggtgaaaga
      181 ggccggggcc acggtcgcca gcgcgattgc gctcgtcgag aagacggaca attgcggcgt
      241 gcgccgcgtc catgacgcaa cgggcgtcac cgtgacgacc ataatgaaga tcgcggtcgg
      301 gaaggacggt gtcagaatcg tctgaagtcg taacccggac ccgcgctccg gagtcctgcg
      361 aaaaaagcgg gagacgtttc ttgtcttgcg ggcacttggc cgctggctcc cgcgggtgac
      421 cttgtcggcg gcgccggaca tagtatcctg gccctcgggt accgcgagcc gggaggagct
      481 gccatgcccg acggtctgag atctaacggg cctgccctca cacgccgtca gttcggcttg
      541 gccgccggcg cgctcacgat cgccttcagc tttgcacccg ccgcggcaga gccggcgaag
      601 cttcccggca gcctcgacca caatcgcatg ctcgacgcct ggctgcgcat cgaggcgagc
      661 ggcgctgtca cggtctgcac cgggaaagtc gagctcgggc agggcattct gacggcgctc
      721 gagcagatcg tcgccgacga gctcgatgtc gcgccgacgc gcatcaggat aatctccggc
      781 gacaccgcgc agacgcccga tgaaggcttc acttccggca gccagtcgat cgagtatagc
      841 gggacggcgc tgcgttatgc ttgcgccgag gcgcgcagca ttctcgtgag cctggcgctg
      901 aagcgcttcc atctcgacgg caagatcgcc gattccgaga tggccgtggt cgacggcacc
      961 atcacggttg ggccgcagaa gcgcaagatc acctattggc agctcgccgc cgacgcgcag
     1021 cgcgagctcc atcgcgaggc caccgccgcc atctcgccga agccggccga ggcgcacaag
     1081 ctcatcggca ccagtcggcc gcgccgcgac atcccggcga aagtgatggg cgcggcgagc
     1141 tatatccagg acatgcggct gccgggcacg ctgttcgggc gcgttgtgcg gccgccgagc
     1201 tatacggccg agctcgaatc cgtcgacacg gcgcgcgtgg agaaactgcc gggcgtcgtc
     1261 gccgtggtcc gcgacgggcg cttcctgggc gtgctcgcca aacgcgagga gcaggcgatc
     1321 gcggcgcgcg aggcgctgcg cagcgccgcc gtgtggcgct cgggcacgcc gctccctccg
     1381 tccgatgcca ttctcgacgc catccaggcg ttccgcaacg acgtgcggga gatcagcgag
     1441 aagggcgagg gccaagacct gccgaaccag gccaattggc gcagcttcgt cgcccgctat
     1501 tcacggccct tcatcgcgca cgcctcgatc ggcccgtctt gcgccatcgc gcagatgagc
     1561 gagggacggc tcacggtctg gacgcacagc cagggcgtct tcccgctgcg gcgcgatctc
     1621 gccaaggcgc tcgatctcga cgagagcagc atccgcgcga ttcacgtcca gggctccggc
     1681 tgctatggac acaacggcgc cgacgacgcg gcgctcgacg cggcgctgct cgcgcgcgcg
     1741 gcgcaggggc ggccggtgaa gctccaatgg atgcgcgacg acgaattcgc ctgggagccc
     1801 ttcggccccg cgatggtcgc ctcggtcggc gcccggctcg acgagcaagg caagatcgtc
     1861 gcctgggcct atgacgtgca cagcaacacc cacagcacgc gtccgggaca gcaagacggc
     1921 tgcaatcttc tcgcggcgtg gcatctggcg accccgaaag cgccgtcgcc gcccgagatg
     1981 atcccgcagc cagcaggcgg cggcgaccgc aatgccattc cgctctacga cttcccttgg
     2041 cagaaggtgc ttcatcattt catccggccg atgccgatcc gcgtctcggc gctgcgggcg
     2101 ctcggtgccc attgcaacat cttcgccatc gaatccttca tggacgagct tgctttggcg
     2161 gccggcgtcg atccggtcga gttccgactg caccatctga gcgatccgcg cggccgcgcc
     2221 gtgattgaga ccgcggccaa agaggcgggc tggacgctgg gcgcggcggg cggcaacggc
     2281 caaggccgcg gcctcggctt cgccaaatac aaaaatcttt cgggctacgt ggctgtggtg
     2341 gccgaagtca cggtcgatcg cgacagcggc gccgtgcgcg tgacgaaagc gacggcggcg
     2401 atcgatgccg gccaggtcgt caatcccgac ggcctcgtca atcagaccga aggcggcatc
     2461 gtccaggcga cgagttggac gctcaaggag gaagtcgcct tcgatgcggc gcacatcacg
     2521 tcgcgcgact ggtcgaccta tccgatcctg accttccccg aggtgccgga gatcaaagtg
     2581 acggtgatcg accggcccgg cgagcgttcg ctcggcgccg gcgaggcggc gcaaggcccg
     2641 accgccgccg ctatcgccaa cgccgtcgct cacgccaccg gcgcacggct gcgcgatctg
     2701 ccgctcctgc ccgagcgcgt gaaggccgcc ctcgggtaac gtcccccacc cgctgatccc
     2761 gactttcacc ggcaagaaag ccgcgcattt gcgctgaatt gtcctctttc tcaattcgtt
     2821 tacctaaatt tgcgaaagtt gccgtggtcg acgcgggaat cacgcgaacg gcgcgcgcca
     2881 tcccatggcc gccgctcgca tcggggagcg tcagaggagc gacaggtgga cgacggcaga
     2941 ggcgcgagag ccgaagcgac agcgccgcgg ggcggcattc cgctcgcgct ggctcctctg
     3001 ctgtcgccct atcgtcgaca cgggcaactt tccgtccgcg tcgagcgcct gcccaacgga
     3061 gcccgtttct ccaaaggaca gaacaacggc gatcgcacct ggtcgctgcg gccggacgag
     3121 ctcgacgacg tgctctacca gccgcccgaa ggcttggaag aaacgcatac cctcgccgtt
     3181 cggatcctga gcctggacgg cgaccagggc tccacgctgg cggtgctcga tttgccggtc
     3241 tcgccggccg atgcgcgagc gatcgtacca ccccctatga cggcgccggg ggcgcctgcc
     3301 ggtaatgcgc cggtccataa tgacggcgag ctacgccgac tccgcgacca gctgacggag
     3361 ctgacggcgc gcctcgcggt ccgcgaggcg gaattggccg aagcgcggca gaaattcgaa
     3421 ctcgcccagg gcgcagggtc gaagcaggcg atcgagggcc agctcgcggc tgctcgtgtt
     3481 ctttgggaag ccgagctcaa ggaccggctc gccgcggcgg cggcggaggc gacgacgaca
     3541 ctggcgacga gccgcgattc ctggcaggcg gaacagggcg gccgcttcgc cgagctcgaa
     3601 aagcgggcgg acgagcagat ccagctggcg caacaccgct ggcagcaaga cgccaaggcg
     3661 gcgatgtcgc acgcggaagc aagctggaag tcgggggaag ccgcgcggct ggcggcggcc
     3721 gaggcgcggt tgcgcgagga atggacgcgc gccctcgccg aggccgaggc ccgcgtccaa
     3781 cgcgccgaga cggcactggc gcaactgcgg cccgaggagc aaatccagag ggcgcgcgac
     3841 gcctggcagc aggaagcgag aaccgcgctg gcaagggcgg aggagagctg gaaggccaac
     3901 gaggcggcgc ggcacgccgc gtccgaagcg cgctggcggg aagaatacag acgctcgctg
     3961 gcggagacgg agatacgcgc cgagcgggct gaagcggcct tggcgcaggc acgccaggaa
     4021 aacgccgcac tgccgctgcc cgagaagagc gcaaaaaccg acgaagcggt ccggctcgcc
     4081 acgaccgaaa cacgccggcg cgaagaggcc gagcgggcgc tggccgagac caggctacgc
     4141 gccgaacgca ccgaagcggc cctggcgcag gcgcagaccc ggtcctcggg cgatgccatc
     4201 gagattcacg tgctgcacga cgagctgcgc gacgtgaaag ccgcccttgc gcttcgcgag
     4261 acgcaattgg cggacgcgcg atccgcagtg gaacggcgcg gcgcgagcg
//
LOCUS       DASYNS010000490         5283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1213299, whole genome shotgun sequence.
ACCESSION   DASYNS010000490 DASYNS010000000
VERSION     DASYNS010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213299"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1443)
                     /gene="hpnJ"
                     /locus_tag="VGP50_15275"
     CDS             complement(16..1443)
                     /gene="hpnJ"
                     /locus_tag="VGP50_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014105350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HEV8028793.1"
                     /translation="MMKTLFLHPPSYEGFDGGAGSRYQARREIRSFWYPTWLAQPAAL
                     VPGSRLIDAPPARMPLDEVLPLARDYELVVLHTSTPSFASDVKVAEALREVNPHLKIG
                     FVGARVAVQPEQSLAASSAIDFVARNEFDFTVKEVAEGRSFDRIDGLSFRTAGGALVH
                     NGERAILENMDQLPFVTDVYKRDLKIENYFIGYLKHPYLSLYTGRGCKSRCTFCLWPQ
                     TVGGHRYRVRSVGHVVEEIRLAQQYFPQVKEFFFDDDTFTDNLPRAEAIARELGKLGV
                     TWSCNAKANVPRDTLKVMRDNGLRLLLVGYESGNQQILHNIKKGMLIDVARRFTKDCH
                     ELGVTIHGTFILGLPGETKETIEETIRFAADINPHTIQVSLAAPYPGTFLHRQAVENG
                     WFDSDHAELVDEHGIQIAPLHYPHLSHQEIFDSVETFYKRFYFRAGKIASIVSEMVRS
                     PEMMVRRLREGVEFFHFLRERGMAH"
     gene            complement(1440..2594)
                     /gene="hpnI"
                     /locus_tag="VGP50_15280"
     CDS             complement(1440..2594)
                     /gene="hpnI"
                     /locus_tag="VGP50_15280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HEV8028794.1"
                     /translation="MNLDLLRLVGWALWAASGAGCLYLLYAGRATRRFAGRPSPLLAP
                     PVPVSVLKPLCGEDQGLDENLRSFCRQDHPRLQVVFGVQDPGDPAIAVVRRIIADAPG
                     ADLVLVVDGGGGAGNRKVANLQNMLPAARYGLIVMADSDMRVRPDYVAEVTAPLADPA
                     TGLVTCLYRGVAAQGVWSRLAALHVNHGFLPQAVVAATFGERNGCFGATIALRRTTLE
                     AIGGFAAVADALADDHALGQAVRRLGLRVVLSPHIVDNIMAEPSLAALFRHELRWART
                     IRMIAPAGFIGSVLTQPMVLSLGALALGVPWAAGLAMLGAALLCRAVMVRMIDHALRL
                     PVTPLWLMPLRDLLSFVVFVASFFTRRVAWRDRTFRIGRKGQLMDGDRPA"
     gene            2822..3601
                     /locus_tag="VGP50_15285"
     CDS             2822..3601
                     /locus_tag="VGP50_15285"
                     /inference="COORDINATES: protein motif:HMM:NF016236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEV8028795.1"
                     /translation="MLQDASATAAKITVLLAALAAGGCATPPSDPAARAEFEHTNDPF
                     EPLNRKIFDVNQVLDRILIKPIAVTYQAAVPEDGRTAIRNFLSNLHEPVVFANNMLQG
                     EFKRAHDTVGRFAMNSTLGVGGFFDLATKAGLEKQSGDFGQTLYSWGVPEGPYLFLPI
                     LGPSDPRDAIGYGVDSLADPYGWAIPGSTITNADYGRFAIGGVDLRAQNIQTLDEVQR
                     SAIDFYSELRSLYRQHRAAELRHGEPPPLPELDEDEPAPKH"
     gene            complement(3695..4306)
                     /locus_tag="VGP50_15290"
     CDS             complement(3695..4306)
                     /locus_tag="VGP50_15290"
                     /inference="COORDINATES: protein motif:HMM:TIGR03481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8028796.1"
                     /translation="MISRRLILAAALAAAAALPLRLAAAESSSPAATVSSFYDTLLGV
                     MKEGPALGFRGRVDRLAPDIRRSFNFALMTRLMVGLQWANLTPEQQQRLVAAFSDFSI
                     AIYASRFDDYSGERFEVDPNPTPTTGGVVVHSKLVKSDGEPVELDYLMHEANDAWQIV
                     DVYLSGTVSELATRRSEFSSVMRNGGPEALVDLLQKKAAELRG"
     gene            complement(4303..>5283)
                     /locus_tag="VGP50_15295"
     CDS             complement(4303..>5283)
                     /locus_tag="VGP50_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEV8028797.1"
                     /translation="QVKSKPQAPPYTNVFAQALIAEAERDERIVAITAAMPSGTGLDK
                     FAERFPSRCFDVGIAEQHAVTFAAGLACEGLKPFAAIYSTFLQRAYDQVVHDVAIQRL
                     PVRFALDRAGLVGADGATHAGSYDLAYLGCLPDFVIMAAADESELMHMVATCAAIDDR
                     PSAVRYPRGEGIGVALPERGTVLPLGKGRVLREGTTVALLSLGTRLQECLKAAAELEA
                     HGLSTTVADARFLKPLDGDLVARLAREHEVLVTVEEGAIGGFASHVLQHLANAGLLDQ
                     GLKLRTLVLPDRFIDHDTPQKQYDEAGLAAKHIAAAALAALGRLRQSARA"
BASE COUNT          933 a         1761 c         1789 g          800 t
ORIGIN      
        1 cagccgccgc aagcgtcagt gcgccatccc ccgctcgcgc aggaaatgga agaactcgac
       61 gccttcgcgc aggcggcgca ccatcatctc ggggctgcgc accatctcgc tgacgatcga
      121 ggcgatcttg ccggcgcgga agtagaagcg cttgtagaag gtctcgaccg aatcgaagat
      181 ctcctgatgc gagagatggg gatagtggag cggcgcgatc tggatcccgt gctcgtccac
      241 cagttccgca tggtcggaat cgaaccagcc gttttcgacc gcctggcggt ggaggaaagt
      301 gccgggataa ggcgcagcga gcgagacctg gatggtgtgc gggttgatgt cggcggcgaa
      361 gcggatcgtc tcctcgatcg tctccttggt ctcgccgggg aggccgagga tgaaagtgcc
      421 gtggatggtc acgccgagct cgtggcaatc cttggtgaag cggcgcgcca catcgatcag
      481 catgcccttc ttgatgttgt gcaggatctg ctggttgccg gattcgtagc cgacgagcag
      541 cagcctcaag ccgttgtcgc gcatcacctt cagcgtgtcg cgcggcacat tcgccttggc
      601 gttacaggac caggtgacgc cgagcttgcc cagctcgcgc gcgatcgctt cggcgcgcgg
      661 caggttgtcg gtgaaggtgt cgtcgtcgaa gaagaactcc ttcacctgcg ggaaatattg
      721 ctgagcgagg cggatctcct cgaccacatg gccgacgctg cgcacgcggt agcgatgtcc
      781 gcccaccgtc tgcggccaga ggcagaaggt gcagcgcgac ttgcagccgc gcccggtata
      841 gagcgagaga tagggatgct tcaggtagcc gatgaagtag ttctcgatct tgaggtcgcg
      901 cttgtagacg tcggtgacga aggggagctg atccatgttc tcgaggatcg cgcgctcgcc
      961 attgtggacg agggcgccgc cggcggtgcg gaagctcagg ccgtcgatgc ggtcgaagga
     1021 ccggccctcg gcgacctctt tcacggtgaa atcgaactcg ttgcgcgcga cgaaatcgat
     1081 cgccgacgag gcggcgaggc tctgctccgg ctgaaccgcg acgcgggcgc cgacgaagcc
     1141 gatcttgaga tggggattaa cttcgcgcag cgcctcggcg accttcacgt ccgacgcgaa
     1201 ggagggcgtc gaggtatgca gcaccacgag ctcgtagtcg cgggccagcg gcagcacctc
     1261 gtcgagcggc atgcgcgcgg gcggcgcgtc gatcagccgg ctgccgggca cgagcgccgc
     1321 cggctgggcg agccaggtgg gataccagaa ggagcggatc tcgcggcgcg cctgatagcg
     1381 cgagccggcc cccccgtcga agccctcgta ggacggcggg tgaaggaaca atgtcttcat
     1441 catgccggtc tgtctccgtc catgagctgg cccttgcggc cgatgcggaa cgtccggtcg
     1501 cgccatgcga ccctgcgggt gaagaaacta gcgacaaaca ccacgaacga caacagatcg
     1561 cgcagcggca tcagccacag gggcgtgacc ggcaggcgca atgcatgatc gatcatacga
     1621 accatgacgg cccggcacag gagggccgcg ccgagcatcg ccaagccggc ggcccatggc
     1681 acgccgagcg ccagcgcccc gagcgatagt accatgggct gcgtgaggac ggagccaata
     1741 aaaccggcgg gcgcgatcat gcggatcgtg cgggcccagc gcagctcgtg acggaacagc
     1801 gcggcgaggc tgggctcggc catgatgttg tcgacgatat ggggcgacag cacgactcgg
     1861 agcccgagcc gccggaccgc ctggcccaag gcgtggtcgt ccgccagcgc atcggccacc
     1921 gcggcgaagc cgccgatggc ctcgagcgtg gtccggcgca gcgcgatcgt ggcgccgaag
     1981 cagccgttgc gctcgccgaa cgtggccgcc accacggcct gcgggagaaa gccgtgattg
     2041 acgtggagcg ccgcgaggcg cgaccagacg ccttgggccg ccacgccgcg ataaaggcag
     2101 gtaacgaggc cggttgccgg atcggcgagc ggcgccgtca cctccgcgac gtagtcggga
     2161 cggacgcgca tgtcgctgtc ggccatgacg atgaggccgt accgggccgc cggcagcatg
     2221 ttctgcagat tggcgacctt gcggttcccg gcgcccccgc cgccatcgac gaccaggacc
     2281 agatcggcgc ccggcgcgtc cgcgatgatg cggcgcacga ccgcaatcgc cggatcaccg
     2341 ggatcctgga cgccgaagac gacctgcagg cgcggatggt cctgccgaca gaaggagcgg
     2401 aggttctcat cgagcccctg atcctcgccg cagagcggct tcagcacact gaccggcacc
     2461 ggcggcgcca gaagcggcga aggccgtccg gcgaagcgac gcgtcgcccg gccggcatag
     2521 aggagataaa ggcagccggc gccgctcgcc gcccatagcg cccagccgac cagcctcagc
     2581 agatcaagat tcatgccggt ttctctcgcc cgcgctcctc gccgatcccg atcgttccgg
     2641 cgaaaccgcg tgtaacttta tcccgacgcc ggatcgacgg cgccggtgtg tcgcaattca
     2701 cgcagcggcc gcgcgcgccc ccgagtcggc ccttagcgca tgaaattgct gttgcattcg
     2761 atggaggcat tgccgacgat gcgccgatcc gtgcgggaac ccccggtcca agaagatgac
     2821 gatgctgcaa gacgcgtccg ccactgccgc caagatcacc gtgttgcttg cagcgctcgc
     2881 ggccggaggc tgtgccacgc cgccgagcga cccggcggcg cgcgcggagt tcgagcacac
     2941 caatgatccg ttcgagccgc tcaaccggaa gatcttcgac gtcaatcaag ttctcgaccg
     3001 catcctgatc aagccgatcg ccgtgaccta tcaggccgcg gtgccagagg acggccgcac
     3061 cgcgatccgg aattttctca gcaacctgca cgagcccgtc gtcttcgcca acaacatgct
     3121 tcagggcgag ttcaagcgcg cgcatgacac cgtcggccgc ttcgccatga acagcaccct
     3181 gggcgtgggc ggctttttcg acctcgccac caaggcgggg ctcgagaagc agagcggaga
     3241 tttcggccag accctctaca gctggggcgt gcccgaaggc ccctacctgt ttctgccgat
     3301 cctcggtccg tccgacccgc gcgatgccat cggctatggc gtcgactccc ttgccgatcc
     3361 ctatggctgg gcgattcccg gcagcacgat caccaacgcg gattacggcc gcttcgccat
     3421 cggcggcgtc gacctgcgcg cccaaaacat ccaaacgctc gacgaagtgc agcgcagcgc
     3481 catcgacttc tattccgagc tgcgcagcct ctatcgccag caccgcgcgg ccgagctgcg
     3541 ccacggcgag ccgccgccgc tgccggagct cgacgaggac gagccggcgc cgaagcatta
     3601 gaggagagaa gcgcacgcgt cgcttgcccc tcggacccgt tttcgcgggg gtgatgaagg
     3661 cgcgcgtggc gataagcggg acgtgctgta gccctcagcc gcggagttcc gctgctttct
     3721 tctgcaacag atcgaccaga gcctcgggac cgccgttgcg catgaccgag gagaactccg
     3781 agcgtctggt cgcgagctcg ctcaccgtgc cgctcagata gacatcgacg atctgccagg
     3841 catcattcgc ctcgtgcatg agatagtcga gctccaccgg ctcgccgtcg ctcttgacga
     3901 gcttgctatg cacgacgacg ccgcccgtcg tcggcgtcgg attggggtcg acctcgaaac
     3961 gctcaccgga ataatcgtcg aaacggctgg cgtagatggc gatgctgaaa tcgctgaacg
     4021 cggcgacgag tcgctgttgc tgctcgggcg tgagattcgc ccattgcagg ccgaccatga
     4081 gccgggtcat gagcgcaaaa ttgaagctgc gccggatgtc cggggcaagt cggtcgaccc
     4141 ggcccctgaa gccgagcgcc ggcccttcct tcatcacgcc cagcaaagtg tcatagaagg
     4201 acgagacggt cgccgccggc gaggacgact cagcggcggc cagccgcaac ggcagcgccg
     4261 cggcggccgc cagggcggcg gcgaggatga gacggcggga aatcatgcgc gggccgactg
     4321 gcgcaggcgt cccagcgcgg caagcgcggc ggccgcgatg tgcttggcgg caaggccagc
     4381 ctcgtcatat tgcttctgtg gcgtgtcgtg atcgatgaag cggtccggca gcaccagggt
     4441 gcggagcttg aggccctggt ccaagaggcc cgcattcgcg agatgctgca ggacgtggct
     4501 cgcaaagccg ccgatcgcgc cctcttccac cgtcaccagc acctcatgct cgcgcgccag
     4561 ccgcgccacg agatcgccgt cgagcggctt gaggaagcgc gcatcggcga ccgtcgtcga
     4621 gaggccatgc gcctcgagct cggccgcggc cttgaggcat tcctggagtc gcgtgccgag
     4681 gctcagcagc gccacggtcg tcccctcgcg cagcacccgg cctttgccca ggggcaggac
     4741 cgtgccccgc tccggcagag cgacgccgat tccttcgccg cgcgggtagc gcaccgccga
     4801 agggcggtcg tcgatcgcgg cgcaggtcgc caccatatgc ataagctccg actcgtccgc
     4861 cgccgccatg atgacgaagt ccggcaggca gccgagatag gccaggtcgt aggacccggc
     4921 atgggtcgct ccgtccgccc ccaccaggcc cgcgcggtcc agtgcaaagc gcaccggcag
     4981 acgctggatc gccacgtcat gcaccacctg gtcgtaggcg cgctgcagga aggtcgagta
     5041 gatcgccgcg aaaggcttca gcccttcgca ggcgaggcca gccgcaaagg tcacggcgtg
     5101 ctgctcggcg atgccgacgt cgaagcagcg ggagggaaag cgctctgcga atttgtcgag
     5161 gcccgttccc gacggcatcg ccgccgtgat cgcgacgatt cgctcgtcgc gctcggcctc
     5221 ggcgatcagc gcctgcgcaa acacattggt gtaaggcggc gcctgcggct tgcttttcac
     5281 ctg
//
LOCUS       DASYNS010000491         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4647526, whole genome shotgun sequence.
ACCESSION   DASYNS010000491 DASYNS010000000
VERSION     DASYNS010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4647526"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VGP50_15300"
     CDS             complement(<1..560)
                     /locus_tag="VGP50_15300"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV8028798.1"
                     /translation="MAPRPSSLDDLRRRIDEIDDRLHDLIMQRAEVIEEIAGTKKRDG
                     IATIRPGREALILRRLVERHRGRFPRAVLVRLWRELLSGTIAMQGNFAVAVYMPRSTP
                     DYWDLARDHFGSHTPMTAFHSASEVLRTLGEGRAAVGVLPMPAEGESAPWWPLLTESG
                     AAVPQIIARLPFAGSGNARVDGGEAVV"
     gene            695..1870
                     /locus_tag="VGP50_15305"
     CDS             695..1870
                     /locus_tag="VGP50_15305"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEV8028799.1"
                     /translation="MSVRASSTTDRDAAREPAVPGRRLVVGEPLMLDCGVALGPFTIA
                     YETYGSLNTERSNAILICHALTGDQYVAGHHPFTGKPGWWETLVGPGKVLDTERYFLI
                     CANVIGGCMGSSGPTEVNPATSKAWGLSFPVITIADMVRAQKRLVDHLGIASLFCVIG
                     GSMGGMQALQWAASFPDSVFSAVPIATAARHSAQNIAFHEVGRQAIMADPDWRGGAYL
                     ETSRRPERGLAVARMAAHITYLSEQALHRKFGRNLQNRQAVAYGFDADFQVENYLRHQ
                     GISFVERFDANSYLYITRACDYFDLAAEHGGVLANAFRNSRTRFCVISFTSDWLYPTS
                     ENRAIVHALNAAGANVSFVEVATDKGHDAFLVEEPEFFATLSGFLDGCARHRGLPPR"
     gene            1867..2520
                     /gene="metW"
                     /locus_tag="VGP50_15310"
     CDS             1867..2520
                     /gene="metW"
                     /locus_tag="VGP50_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEV8028800.1"
                     /translation="MSADGSLPASAATNKHLRGDLRLVADMVEPGARVLDIGCGDGEL
                     LSFLTQQKRVDGRGMELSQSGVNACVRHGLSVIQGDADHDLEDYPSGGFDYAVLSQTL
                     QATRQPRHVIEELVRIGRRAIVSFPNFGHWRIRLNLLVNGRMPKTPLLGQAWHDTPNI
                     HLCTILDFVALCDELGVVIERSITLDRAGLPYGLNPRGGLANLMAEQALFLLRRTRA"
     gene            complement(2523..>2918)
                     /locus_tag="VGP50_15315"
     CDS             complement(2523..>2918)
                     /locus_tag="VGP50_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HEV8028801.1"
                     /translation="LKEIWRVMAGGGRLMAIVPNRRGIWARIDSTPFGQGHPFTPGQL
                     SRLLREADFTPERVGAALFVPPTGSRMMLRSASAIERIGERWFTTFAGVSIVEASKQL
                     YAKPAIKRKEKPRRVYVPAVHGAPRATRG"
BASE COUNT          480 a         1028 c          911 g          499 t
ORIGIN      
        1 accaccgcct cgccgccatc gacgcgcgca ttgccgctcc cggcgaaagg caatcgggcg
       61 atgatctgcg gcacggcggc gccgctttcg gtcaacagcg gccaccacgg cgcgctctcg
      121 ccttcagcgg gcatcggcaa cacgcccact gcggctcgcc cctcgccgag cgtgcgcaac
      181 acctcgctgg cagagtgaaa ggccgtcatc ggtgtgtggc tgccaaagtg gtcgcgcgcg
      241 agatcccaat agtccggtgt gctccgcggc atgtacacgg cgacagcgaa gttgccctgc
      301 atcgcaatgg tgccgctcag gagctcgcgc caaagacgca ccaggacggc acgcggaaaa
      361 cggccgcggt ggcgctcgac gagacggcgc aggatcagcg cttcgcgccc ggggcggatg
      421 gtggcgatac catcgcgttt tttcgtgccg gcgatctcct cgatgacctc cgcacgctgc
      481 atgatgaggt cgtggagccg gtcatcgatc tcgtcgatgc ggcggcgcag atcatcgagg
      541 ctcgaaggac gcggggccat ggcggatatc gtcaaaatca gaccgctgga gagccgatag
      601 agatagttcg ccccggtgcg gaaagcaaag aaaacattga catctttcgc ccttgatccc
      661 tagctatgcg gctctcctca acctccgccg gcccatgagc gtgcgagcct cttccaccac
      721 cgaccgcgac gccgcccggg agcccgccgt tccgggccgc cgcctcgtcg tcggcgagcc
      781 gctgatgctc gattgcggcg tggcgctcgg gcctttcacc attgcctacg agacctatgg
      841 cagcctcaac accgagcggt cgaacgccat tctgatctgc cacgcgctga cgggcgacca
      901 gtatgtcgcc gggcatcatc cgttcaccgg caaacccggc tggtgggaga ctttggtcgg
      961 ccccgggaag gtcctcgata ccgagcgcta tttcctcatc tgcgccaacg tgatcggcgg
     1021 ctgcatggga agcagcggcc cgaccgaggt gaaccccgcg acaagcaagg cctgggggct
     1081 gtcgtttccg gtcatcacca ttgccgacat ggtgcgggcg cagaagcggc tcgtcgacca
     1141 tctcggcatc gcctcgctgt tctgcgtcat cggtggatcg atgggtggta tgcaggcctt
     1201 gcaatgggcc gcttcctttc ccgacagcgt cttctcggcg gtaccgatcg ccacggcggc
     1261 gcgccactcc gcgcagaaca tcgcgttcca cgaggtgggg cggcaggcga tcatggcgga
     1321 ccccgactgg cgcggcggcg cctatctcga gaccagccgg cggcccgagc gcggcctcgc
     1381 tgtcgcacgc atggcggcgc atatcaccta cctgtccgag caagcgctgc accgcaagtt
     1441 cggccgcaac ctgcagaacc gccaggccgt ggcctatggc ttcgacgcgg atttccaggt
     1501 ggagaactat ttgcggcatc agggcatctc tttcgtcgag cgcttcgacg ccaactccta
     1561 tctctacatc acccgcgcgt gcgactattt cgacctcgcg gctgagcatg gcggcgtgct
     1621 cgccaatgcc ttccgtaatt cgcgcacgcg gttctgcgtc atctccttca cgagcgactg
     1681 gctttatccg acgagcgaga accgcgccat cgtccatgcg ctcaacgccg ccggcgccaa
     1741 tgtcagtttc gtcgaagtgg caaccgacaa ggggcacgac gccttcctgg tcgaggagcc
     1801 ggaattcttc gcgaccttga gcggcttcct cgacggctgt gcccgccatc gcggtctccc
     1861 cccgcgatga gcgccgacgg cagtctcccc gcatcggccg ccacgaacaa gcacttgcgc
     1921 ggcgatctcc ggctcgtcgc cgatatggtc gagccgggcg cacgcgtgct cgacatcggc
     1981 tgcggcgacg gcgagctctt gagcttcctc acccagcaga agcgcgtcga cgggcgcggc
     2041 atggagctct cgcagtccgg ggtcaacgca tgcgtgcgcc atggcctttc ggtcatccag
     2101 ggcgacgccg atcacgacct cgaggactat ccttccggcg gtttcgacta tgcggtgctg
     2161 agccagacgc ttcaggcgac gcgccagccg cgccatgtca tcgaggagct cgtgcgcatc
     2221 ggccgccgcg ccatcgtttc cttccccaat ttcggccatt ggcgcatccg cctcaacctc
     2281 ctcgtcaacg gacgcatgcc gaaaacgccg ctcctcggtc aagcctggca cgacacgccc
     2341 aacatccacc tctgcacgat tctcgatttc gtcgctttgt gcgacgagct tggcgtcgtc
     2401 atcgagcgca gcatcaccct cgaccgcgcc ggccttccct atggcctgaa cccgcgcggc
     2461 ggccttgcca acctcatggc cgagcaggcg ctcttcctgc tgagacgaac ccgcgcctag
     2521 gatcagccgc gcgtcgcgcg cggcgcgccg tggaccgccg gcacatagac acgccgcggc
     2581 ttctctttgc gcttgatggc gggctttgcg taaagctgct tcgaggcctc gacgatcgag
     2641 acgccggcga aggtggtgaa ccagcgttcg ccgattcgct cgatggcact ggccgagcgc
     2701 agcatcatgc gcgagcccgt cggcggcacg aagagcgcgg caccgacccg ctcgggcgta
     2761 aaatcggctt cgcgcaagag gcgcgagagc tgaccgggcg tgaacggatg cccttgaccg
     2821 aagggcgtgc tgtcgatgcg cgcccagatg ccgcgccggt tcggcacgat cgccataagc
     2881 cgcccgccgc ccgccatcac gcgccagatc tccttcag
//
LOCUS       DASYNS010000492         5619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5115731, whole genome shotgun sequence.
ACCESSION   DASYNS010000492 DASYNS010000000
VERSION     DASYNS010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5115731"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VGP50_15320"
     CDS             <1..210
                     /locus_tag="VGP50_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028802.1"
                     /translation="LRLLATWLGLERLVIGRRGALATELRAQLPDARRARRRGGGAES
                     SSMAGAGRTPVPIAEREDVSAESAH"
     gene            236..1402
                     /locus_tag="VGP50_15325"
     CDS             236..1402
                     /locus_tag="VGP50_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV8028803.1"
                     /translation="MIATITMPRLLYVGGGAIAEVTTALQKLGARRPLIVSDPFMERS
                     GLLAKLTDRLDAASVPWRLFAETVPDPTTAVVEAGVALLKAGNYDCLIALGGGSPIDT
                     AKAMSVLAANGGAMRDYKVPNEIPALGLPLIAIPTTAGTGSEVTRFTVITDSECDEKM
                     LIMGVACCPVAAIVDYELTFSMPLRLTADTGIDSLTHAIEAYVSRKASPLTDGLARAA
                     MALIAKHIRAACREPQNRAARAAMMVGATQAGMAFSNASVCLVHGMSRPIGAFFHVPH
                     GLSNAMLLPAITAFSAPAALERYADCARAMGIAAEGEGSQSAVARLLDELRALNDDLQ
                     VPTPKAYGIAAARYEELLPVMASQALASGSPANNPRIPTADEIIDLYKQVYARH"
     gene            1510..3717
                     /locus_tag="VGP50_15330"
     CDS             1510..3717
                     /locus_tag="VGP50_15330"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEV8028804.1"
                     /translation="MAEVPGQLLAPRLAGHTLTLPLYAATLFTSALLLFWIQPLVAKM
                     MLPLLGGAPAVWNTAMMVFQLLLLAGYGYAHLLSSRIRPAHQPWVHGAVVLAAFAVLP
                     VAIGDAQPSGGASPILWLTLRLLACVGLPFFALSASAPLLQSWFARSGHHSADDPYFL
                     YGASNLGSLLALVAFPLLLEPWFTLGTQSRLWMALYGLLIVLVGACALSSAGGVRSVP
                     LSLPGAADGSVKLGQRLHWIALAFVPSSLLLGVTTFITTDVAAAPLLWVVPLALYLLS
                     FVFAFARQPPVRPGWLAIGQAVGTCVVVLLFVLPTKPLGATMAVHYAAFFVTALMCHA
                     VLAAHRPAAAGLTSFYVCISLGGALGGIFNALLAPLLFSSSYEYPLMLVVACLLRPAA
                     MRQARGLIDLVAPLALLVGVLALIRYWAALAALSPVALAAAVLAVPAAVFALNGRALR
                     FALAVAAVLVPVVATRSIEGVIYQERSFFGVNRVKLDQESQTVDLMHGTTVHGAEFLD
                     RARWREPLSYYAREGPIGQLFAAWREVWPGPRQIAVVGLGTGALACYARPSDAWTFYE
                     IDPAVEHIARDTRFFHYLESCGVAAKVVLGDGRLSLRSASRRYDALILDAFSSDAIPL
                     HLLTREAVLLYFDHVVDNGLVVLHISNKHLELAPMLKALARDLGLAERHELFVPPPTA
                     AASAVSMSEWVVLARRDADLAFLGGAPLWGVDTSSRVVQPWTDDFSNILGVMR"
     gene            complement(3722..4945)
                     /locus_tag="VGP50_15335"
     CDS             complement(3722..4945)
                     /locus_tag="VGP50_15335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8028805.1"
                     /translation="MMSRSRRYEFTLVGLLVLLWGSVGLNRIGIGAIFPEIKPAFGMS
                     NLETSLLISGTSVTWAISSWAGGWLSDRYGRRWVLLPAALWICVVTAAMGITWGFWSM
                     FIVRDLLGIGDGIGWSVGESAISEESAPQRRGLNQALFTAGYTFVGAAIGPLIITRLS
                     LALGWRWVFPIIGGATLLVVAGLAFAMREPLERKARRTHDWRAGFIALRRPSLAYITV
                     MGCAILAWLQVSIGFDQLFLTEVRGFSKLDAGSIASVWGFAGTAGQIILPLASDFWGR
                     RPVTLVSALASAAALAAYISGGAGMTGMQILLGISGFFGFGVLPIVLATCVSEAVPEE
                     IRGAALGVTNFFAVIIGTTLMPVIAGRVADHSGLAAALWIAVAAQLAVAALIPAINET
                     APRVVARLAALRTAT"
     gene            complement(4968..5321)
                     /locus_tag="VGP50_15340"
     CDS             complement(4968..5321)
                     /locus_tag="VGP50_15340"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEV8028806.1"
                     /translation="MAEIEQWVRVASRADLGEGEMLGVSAAGREIALYDVGGQVYATD
                     NVCTHAFAMLTDGWLDGDEIECPLHGGRFNVISGKGLGPPIPCDVKIYKLRIAGDAIE
                     IAIPMQPVALGPGGA"
BASE COUNT          820 a         1987 c         1854 g          958 t
ORIGIN      
        1 ctgcggcttt tggcgacatg gctcggtctc gagcggctcg tcatcggccg gcgcggcgcg
       61 ctcgcgacgg agctgcgggc gcagctgccg gacgcgcgcc gcgcgcgccg ccgcggcggc
      121 ggcgcggagt cgagcagcat ggcaggggcc gggcgcaccc ccgtcccgat cgcggagagg
      181 gaagacgtgt ccgcagagag tgcgcattga cgctgagaac ccgaggggag acgccatgat
      241 cgccaccatc accatgccac ggctgctcta tgtcggcggc ggcgccatcg cggaagtgac
      301 gaccgcgctc cagaaactcg gcgcgcggcg gccgctcatc gtcagcgacc cgttcatgga
      361 gcgctccggc ctcctcgcca agctcaccga ccggctcgac gcggcaagcg ttccctggcg
      421 cctgttcgcg gagaccgtgc ccgaccccac gacggccgtg gtcgaggcgg gcgtcgccct
      481 cctcaaagcc ggcaattatg actgtctcat cgcgctgggc ggcggcagcc ccatcgacac
      541 ggccaaggcc atgtcggtgc tggccgccaa tggcggcgcg atgcgcgact acaaggtgcc
      601 gaacgagatc cccgcgctcg ggctgccgct catcgccatc ccgacgaccg ccggcaccgg
      661 ctccgaggtg acgcggttca ccgtcatcac cgacagcgag tgcgacgaga agatgctgat
      721 catgggcgtc gcctgctgcc cggtcgcggc catcgtcgac tacgagctga ccttctccat
      781 gcccttgcgc ctcacggccg ataccgggat agacagcctc acccacgcca tcgaggccta
      841 tgtgagccgc aaggcgagcc cattgacgga cggccttgcc cgcgcggcga tggcgctcat
      901 cgccaagcat atccgcgccg cctgccgcga gccgcagaac cgcgccgccc gcgcggcgat
      961 gatggtgggt gcgacccagg ccggcatggc cttctccaac gcgagcgtgt gtctcgtcca
     1021 cggcatgagc cggccgatcg gtgctttctt tcatgtgccg catggcctct ccaacgccat
     1081 gctgctgccg gcgatcaccg ctttctcggc gccggccgcg ctcgagcgtt acgccgattg
     1141 tgcccgcgcc atgggcatcg ccgcggaagg cgagggcagc cagtcggccg tcgcgcgtct
     1201 tctcgacgaa ctccgcgcgc tcaacgacga tctccaagtg ccgacgccca aggcctatgg
     1261 catcgccgcc gcgcgctatg aggagctgct gccggtgatg gcgagccagg cgctcgcctc
     1321 cggctcgccg gccaacaatc cccgcattcc caccgccgac gagatcatcg atctctataa
     1381 acaggtctac gcgcgacact aaattgggga gagataaaac tatttttaca attatttagg
     1441 ataattgctt tagacccgac gccaagctga cggcagcgct caccgacggc agcgaggagg
     1501 gccatgccca tggcggaggt cccgggtcag ctattggccc cgcgcttggc cggtcacacg
     1561 ctgacactgc cgctctatgc cgcgacgctg ttcaccagcg ctctgctcct cttctggata
     1621 cagccgctgg tcgccaagat gatgctgccg ctgctgggtg gcgcccccgc cgtctggaac
     1681 acggcgatga tggtgttcca gctgctgctg ctggccggct acggctatgc ccatctcctg
     1741 agctcacgca tccgcccggc gcatcagcct tgggtgcatg gcgccgtcgt cctggcggcc
     1801 ttcgccgtgc tgccggtcgc gatcggtgac gcgcagccct ccggcggcgc gtcgcccatc
     1861 ctttggctga cgcttcggct ccttgcgtgt gtcgggctgc cgttcttcgc gctctcggca
     1921 tcggcgccgc tgctgcagag ctggttcgcg cggagcggac atcacagcgc cgacgacccc
     1981 tatttcctct acggcgcgag caacctcggc agcctcctgg ccctggtggc gttcccgctg
     2041 ctgctcgagc cttggttcac gctcggcaca cagtcgcgac tgtggatggc gctctacggc
     2101 ctgctgatcg tgcttgtcgg cgcctgcgcg ctgtcgtcgg cgggaggcgt ccgcagtgtg
     2161 cccctgtcct tgcctggcgc ggcggacggc agcgtgaagc tcgggcagcg cctccattgg
     2221 atcgcgctcg ctttcgtccc gtcgagcctc ttgctgggcg tcaccacctt catcaccacc
     2281 gacgtcgccg ccgcgccgct gctctgggtg gtgccgctgg ccctctatct gctgtccttc
     2341 gtgtttgctt tcgcccgcca gccaccggtt cggccgggct ggctcgccat cggccaagcc
     2401 gtcggcacct gcgtcgtggt gctactgttc gtgctgccga cgaagccgct gggcgcaacc
     2461 atggcggtgc attacgcggc cttcttcgtc acggcgctca tgtgccatgc cgtgctcgcg
     2521 gcgcatcgcc cggcggctgc cgggctcacg agcttttacg tctgcatctc gctgggcggc
     2581 gcgctcggcg ggatcttcaa tgcgctgctg gcgccgctgc tgttctcgtc gagctacgaa
     2641 tatcccttga tgctcgtcgt cgcctgcctg ttgcgccccg cggccatgcg ccaggcacgc
     2701 gggctcatcg acctcgtggc gccgctggcg ctgctcgtcg gcgtgctcgc gctgatccgc
     2761 tattgggcgg cgcttgcggc gctgagcccc gtcgccctgg cggctgccgt gctcgccgtt
     2821 ccggccgcgg tcttcgcgct gaacggtcgc gccttgcgct tcgcgctggc ggtggcggcg
     2881 gtgctggtac cggtggtcgc cacgcgcagc atcgagggcg tgatctatca ggagcgaagt
     2941 ttcttcggcg tcaaccgcgt caagctcgac caggaatcgc aaaccgtcga tctcatgcat
     3001 gggacgacgg tccacggcgc ggagttcctc gaccgcgcgc gctggcgcga gcccctcagc
     3061 tactacgccc gcgagggtcc tatcggccag ctcttcgcgg cctggcgcga ggtgtggccg
     3121 gggccgcggc agatcgccgt cgtcggcctc ggcaccggcg cgctcgcctg ctatgcgcgt
     3181 ccgagcgatg cctggacctt ctacgagatc gatccggcgg tcgagcatat cgcgcgcgac
     3241 acgcgcttct ttcattatct cgagagctgc ggcgtggcgg cgaaggtcgt cctcggcgac
     3301 ggccggctgt ccttgcgttc ggcctcgcgg cgttacgacg ccctgatcct cgatgccttc
     3361 agctcggacg ccatcccgct ccatctgctg acacgcgagg ccgtgctgct ctatttcgat
     3421 cacgtcgtcg acaatggatt ggtcgtgctg cacatcagca acaagcatct ggagcttgcg
     3481 ccgatgctga aggcgctggc gcgcgatctc ggcctcgccg agcgccatga actcttcgtg
     3541 ccgccgccga ccgctgcggc ctcggccgtc agcatgtccg aatgggtggt gctggcgcgg
     3601 cgcgacgccg acctcgcttt cctcggcggc gcgccgctct ggggcgtcga cacctcgagc
     3661 cgcgtggtgc agccctggac cgacgatttc tcgaatattc tcggcgttat gcggtgaacg
     3721 cttaggtagc cgtccgcagc gcggccaggc gtgccacgac gcgtggcgcg gtctcgttga
     3781 tcgctgggat caaggccgcg acggcgagct gcgccgcgac ggcgatccac agggctgcgg
     3841 caagaccgct atggtcggcg acgcgcccgg cgatcacggg catcagtgtc gtgccgatga
     3901 tcacggcgaa gaaattggtg acgccgagcg ccgcgccgcg aatctcctcg ggcaccgcct
     3961 cgctgacgca ggtcgcgagc acgatcggca gcacgccgaa gccgaagaag ccgctgatgc
     4021 cgagcaggat ctgcatgccc gtcatcccgg cgccgccgct gatataggcg gcgagcgccg
     4081 cggcggaggc aagcgcgctc accagcgtca ccggacggcg accccagaag tcggaggcga
     4141 ggggcaggat gatctggccg gccgtcccgg caaagcccca gacggacgcg atcgatcccg
     4201 cgtcgagctt gctgaaccct ctgacctccg tgaggaagag ctggtcgaag ccgatcgaga
     4261 cctggagcca ggccaggatc gcgcaaccca tgaccgtgat ataggcgagc gaggggcggc
     4321 ggagcgcgat gaaaccggca cgccagtcat gcgtccgccg cgccttgcgc tcgagcggct
     4381 cgcgcatcgc aaaggcgagg ccggccacga ccaggagggt ggctccgccg atgatgggga
     4441 agacccagcg ccagcccagc gccagcgaaa gccgcgtgat gatgagcggg ccgatcgcgg
     4501 cgcccacgaa ggtgtagccc gcggtaaaga gcgcctggtt caggccgcga cgttgcggag
     4561 ccgattcctc gctgatcgcg gattcgccga ccgaccagcc aatgccgtcg ccgatgccga
     4621 gcaggtcgcg gacgatgaac atcgaccaga agccccaggt gatgcccatg gccgcggtga
     4681 cgacgcagat ccacagcgcc gccggcagca gcacccaacg ccgcccgtag cggtcggaaa
     4741 gccagccgcc ggcccagctg ctgatcgccc aggtcaccga cgtgcccgag atgagtagcg
     4801 acgtctcgag attgctcatg ccgaaggcgg gcttgatctc gggaaaaatc gcgccgatcc
     4861 cgatgcggtt gagcccgacg ctgccccaga gcagcacgag cagaccgacc agcgtgaatt
     4921 cgtagcggcg cgatcgcgac atcattggcg ctcccccgca cgctcttcta tgccccgccg
     4981 ggtcccaacg ccactggttg catcgggatg gcgatctcga tcgcgtcgcc ggcgatgcgc
     5041 agcttgtaga tcttgacgtc gcaagggatg ggcgggccga ggcctttgcc gctgatgacg
     5101 ttgaaccgtc cgccatggag cgggcactcg atctcgtcgc cgtcgagcca gccatcggtc
     5161 aacatcgcga aggcgtgcgt gcaaacgttg tcggtcgcat agacctggcc gcccacgtca
     5221 taaagcgcga tctcccttcc ggcggcgctg acgcccagca tttcgccctc gccgagatcg
     5281 gcgcgcgatg ccaccctgac ccactgctct atctcagcca tccgcccgca cccccctgat
     5341 ccggggcgcg atcttgccgc gtcgcgccgc tgcgagtcag caggattttg tggtcgacag
     5401 gacggccttc tcacgccgga gcatggcggt tgtcacggcg atgctatatg ggctacactg
     5461 ttaaggtgtg gccaggagag ggctttgcct gaaatgtcgt cctcggacca agggccgccc
     5521 gccgattgtg atgcacgcat cgcgcgcctt tacgcctatt ggcgctcgct ctgccccgcc
     5581 tcgggcggcc ttcctggtcg acagcacctt gatcccggc
//
LOCUS       DASYNS010000493         6264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2776490, whole genome shotgun sequence.
ACCESSION   DASYNS010000493 DASYNS010000000
VERSION     DASYNS010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2776490"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1588)
                     /locus_tag="VGP50_15345"
     CDS             complement(110..1588)
                     /locus_tag="VGP50_15345"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV8028807.1"
                     /translation="MDQAKLERFIDRCWDDEVVPRLVEYIRIPNKSPAFDPQWAAHGH
                     MDAAVALFEGWARRQLGALAGATVEVVRLPGRTPLILIDIPGATRSVVPGATRSVVAR
                     DATRRADDCVLLYGHLDKQPEMTGWADGTGPWIPVLRDDKLYGRGGADDGYAMFGALS
                     AILALREQKVPHARAVLLIEACEESGSYDLPHYVDHLAGRLGTPSLVVCLDSGCGNYD
                     QLWMTTSLRGLVSGTLTVEVLTEGVHSGDASGIVPSSFRILRQLLSRLEDETTGAIRV
                     AALYADIPADRRAQAERAAAALGDDVYTKFPLVPGLAPVATDPTELVLNRTWRPQLAV
                     TGMAGLPPPGNAGNVLLPFTAAKVTVRLPPTLDGPRAVELVGKLLEADPPYGARIRFD
                     GKESSTGWNAPALAPWLGRSLADAAEAAFHAPPAYMGEGGTIPFMAMLGEKFPQAQFV
                     VTGVLGPQSNAHGPNEFLHIPTAKRLSAVIARVLADHHAKAA"
     gene            1717..2472
                     /locus_tag="VGP50_15350"
     CDS             1717..2472
                     /locus_tag="VGP50_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028808.1"
                     /translation="MTPEKPPFAVDIHGLSKRFDRPAVDGLDLHIRAGEFYALLGPNG
                     AGKTTTLRMLTGLLRPDSGAIAIFGIDALADPVAAKRITAWLSEEPMIYDKLTPYEYL
                     EFVAGLWGIEAALAETRATELLRWLGLVPHAHERCESFSRGMRQKVALAGALVHEPKL
                     IVLDEPLTGLDAGSARDVKDMLRARVAGGSTVIMTTHILEVAERMADRIGVIRGGKLI
                     AEGTLEELRQRVGHSAISLEDTFLTLVAREPAT"
     gene            2469..3998
                     /locus_tag="VGP50_15355"
     CDS             2469..3998
                     /locus_tag="VGP50_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028809.1"
                     /translation="MSQPGTVLWLARHELRVTWRDWIAMMTAGRRRRFRRVAIWLVIF
                     AILMHLLAFSMVARYADVGLDAGKSALVAITGSALLSWCLMLSQAMESVTRAFYTRAD
                     LDLLLSSPIAARRVFGVRIGAMALSIAIMAGLLAAPFINAIALVGGARWLAAYGVVVA
                     LALTATALAVALTVTLFRLIGARRTRVAAQIVAAVIGAGFVIGLQAAAILSYGTLSRV
                     AFLQSATVLGHAPAVDSILWWPARAVLGDVTALAALLATSLLLLAAAILLFAPRFGDH
                     AIAAAGVSQQSVARRHRRSGFRRVSPAGALRAKEWALLRRDPWLISQTLMQLLYLLPP
                     ALLLWFSYRDHNGGTALLVPVLIMAAGQLGGGLAWLAISGEDAPDLVATAPVSAGRAL
                     TAKVEAVLGGIAMVFGPFALALAFVAPIEATVAVGGILLAAASATAIQLAFRTQAKRS
                     HFRRRQISSRIATFAEALSSIGWAATGAFAAAGTWVAVIPGVLALAILGGAWLISPAR
                     G"
     gene            complement(4259..5206)
                     /locus_tag="VGP50_15360"
     CDS             complement(4259..5206)
                     /locus_tag="VGP50_15360"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV8028810.1"
                     /translation="MSKAARDPDRRRILAWGAGLAASAWIGGSPAGAAEQTGAGALIM
                     RKIPHSGEAVPVIGLGTSEVFDIGSDAAERRACAEVIRRLIDGGGSVIDTAPSYGRAE
                     AVVGDLVAETGLRGRTFLATKLEEYDRATGPGEVRASLQRLRTDKVDLMQLHNVSDPR
                     QDLALLREWKVQGLCRYIGITTSFGGDFDAVEAVLKREKPDFLEVNYSLADRDAEQRV
                     LPAAAAIGAAVLVDLPLARGRLFRAVRGKPLPDWAKDIDAASWAQVFLKYILGHPAIT
                     AVIPGTNNPDHMADDLGAGRGRVPDEAQRRKMIAFFQSL"
     gene            complement(5203..>6264)
                     /locus_tag="VGP50_15365"
     CDS             complement(5203..>6264)
                     /locus_tag="VGP50_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028811.1"
                     /translation="RFIPIVYHFFVANLALFWLLLTLGVAPVVMAWIFFVWISVFNLF
                     AVSVFWSFMADLFRSEQSKRLFGLIGAGGTAGSILGPVIVLGLADALGPVNLLIIAGL
                     LLELAVLCAHRLERAAGSVAGAQEAAAIGAARVGGSWFAGITQLFASPYLAGIALWVS
                     LLSFAGTILYFEQANLVAAARQTAAAQTQFFARIDLYVAIATIVVQAFVSGPLVKLFG
                     VAAALAVQPVVFVLGFAALAVFPNVTVVLVFQTIQRTANFAFSNLGRQILFTPVPRED
                     KYKTKNVIDVVVFRGSDALYGWVFGVLRDAGLALAAISGIAIPLALGWAGLALALGRG
                     EARRAAATGQDDVVAGEAP"
BASE COUNT          977 a         2129 c         2195 g          963 t
ORIGIN      
        1 gttcccgacc aaggcatcag cgttccgaat ttgcggcgcc ccacctcacc ccagccctct
       61 ccgcccctcg ggggcggaga gggagtcacg cgtgatgtgt gaatcgacct tacgcggcct
      121 tcgcgtggtg gtcggcgagg acgcgggcga tgacggcgga gaggcgcttc gcggtcggga
      181 tgtggaggaa ttcgttcggc ccatgggcgt tcgactgtgg cccgagcacg cccgtgacga
      241 cgaactgcgc ttgcgggaat ttctcgccca gcatggccat gaacgggatc gtcccgcctt
      301 cgcccatata ggcgggcggc gcatgaaagg ccgcttccgc cgcatcggcg agggagcggc
      361 cgagccacgg cgccaaggcc ggcgcgttcc agccggtgga tgattccttg ccgtcgaagc
      421 ggatgcgcgc gccatagggc ggatcggcct cgagcagctt gccgacgagc tcgaccgcgc
      481 gcgggccatc gagagtcggc ggcagccgca ccgtgacctt ggcggcggtg aaaggcagga
      541 gcacgtttcc ggcattgccc ggcggcggca atcccgccat gcccgtgacg gcgagctgcg
      601 gccgccaggt gcggttgagc acgagctcgg tgggatcggt ggcgaccggc gcaagaccgg
      661 ggaccagcgg gaatttggtg tagacgtcgt cgccgagcgc ggccgctgcc cgctccgcct
      721 gggcacggcg atcggccggg atgtcggcgt aaagcgccgc gacgcggatc gcgcccgtcg
      781 tctcgtcctc gaggcgcgac agaagctggc gcaggatgcg gaagctcgac ggcacgatgc
      841 ccgaggcgtc gccggagtgg acgccctccg tcagcacctc gacggtgagc gtgcccgaga
      901 cgaggccgcg cagcgatgtc gtcatccaga gctgatcgta attgccgcag ccggaatcga
      961 ggcacaccac gagcgacggg gtgccgagtc gcccggcgag atggtcgaca taatgcggca
     1021 agtcgtagct gccggactcc tcgcaggctt cgatcaacag gacggcgcgg gcatgcggga
     1081 ctttctgctc gcgcaaggcg aggatcgcgc tcagggcacc gaacatggca tagccgtcat
     1141 cggcgccgcc gcggccatag agcttgtcgt cccggaggac cgggatccag gggccggtgc
     1201 cgtcggccca gccggtcatc tccggctgct tgtcgagatg accatagagc agcacgcaat
     1261 cgtccgcgcg acgcgtggcg tcgcgggcga cgacgctgcg cgtcgcgccg gggacgacgc
     1321 tgcgcgtcgc gccggggatg tcgatcagga tgagcggcgt gcgccccgga agccgtacca
     1381 cctcgaccgt cgcacccgcg agcgccccaa gctgccggcg cgcccagccc tcgaagagcg
     1441 ccacggccgc gtccatatgg ccgtgcgccg cccattgcgg atcgaaggcg ggtgatttgt
     1501 tcggaatgcg gatgtattcg acgaggcggg ggaccacctc gtcgtcccag caccggtcga
     1561 tgaaccgctc aagcttcgcc tggtccatgg cgtcgatcct tctgccgaca tatccacata
     1621 ttatccacat atagcgtgcc gggtgccgtc cgccagtcca ggttgcggcg gcgtgattca
     1681 ccacgcgtaa acttctgctt aataaactgg cgcgcgatga cccccgaaaa gccccccttt
     1741 gcggtcgaca tccacggcct ttcgaagcgc ttcgaccggc cggccgtcga tggcctcgac
     1801 ctccacatcc gcgccggcga gttctatgcc ctcctcgggc ccaacggcgc cggaaagacg
     1861 accacgctcc gcatgctgac cgggctgctc cggcccgaca gcggcgcgat cgcgatcttc
     1921 ggcatcgatg ccctggccga cccggtggcc gccaagcgga tcaccgcctg gctctccgaa
     1981 gagccgatga tctacgacaa gctcacgcct tacgagtatc tcgaattcgt cgccggcttg
     2041 tggggcatcg aggcagcgct ggccgagacg cgcgccacgg agcttctccg ctggctcggc
     2101 ctcgtcccgc atgcccatga gcgctgcgag agcttctcgc gcggcatgcg gcagaaagtg
     2161 gcgctcgccg gcgcgctcgt ccatgagccg aagctcatcg tcctcgacga gccgctgacc
     2221 ggactcgacg ccggctcggc gcgcgacgtc aaggacatgc tgcgcgcacg ggtcgcgggc
     2281 ggcagcaccg tcatcatgac gacgcatatc ctcgaagtgg cggagcgcat ggccgaccgc
     2341 atcggcgtga tcagaggcgg caagctcatc gccgagggca cgctcgagga gctgcggcag
     2401 cgcgtcggcc acagcgccat cagcctcgaa gacacctttc tcacgctcgt cgcgcgcgag
     2461 ccggcgacgt gagccaaccc ggcacggtgc tctggctggc gcgtcacgag ctccgggtca
     2521 cctggcgcga ctggatcgcc atgatgacgg cggggcggcg ccggcgcttt cgccgcgtcg
     2581 ccatctggct ggtcatcttc gccatcctga tgcatctcct cgccttctcg atggtggcgc
     2641 gctacgccga tgtcgggctc gatgcgggaa aatcggcgct ggtcgccatc accggcagcg
     2701 cgctcctctc ctggtgcctg atgctgtcgc aggccatgga atcggtgacg cgcgccttct
     2761 acacccgcgc cgatctcgac ctgctgctct cctcgccgat cgcggcgcgg cgcgtcttcg
     2821 gcgtgcgcat cggcgccatg gcgctgtcga tcgcgatcat ggcgggactg ctcgccgccc
     2881 ctttcatcaa cgccatcgcg ctcgtcggcg gcgcgcgctg gctcgccgcc tatggcgtgg
     2941 tcgtcgcgct ggcgctgacg gcaaccgcgc tcgccgtggc gctgaccgtg acgcttttcc
     3001 gcctgatcgg cgcgcggcgc acacgggtcg cggcgcagat cgtcgcggcg gtgatcggcg
     3061 ccggcttcgt catcgggctg caggcggcgg cgatcctgtc ctatggcacg ctgtcgcgcg
     3121 tcgccttcct gcaatcggcg accgtgctcg ggcacgcccc cgcggtcgac agcatcctgt
     3181 ggtggccggc gcgcgccgtg ctcggcgacg tcacggctct ggcagcgctg ctcgcgacga
     3241 gcctcctgct gctggccgcg gcgatcctgc tcttcgcgcc gcgcttcggc gatcacgcca
     3301 tcgccgccgc cggcgtctcg cagcagagcg tcgcgcgccg gcaccgccgc tcgggcttcc
     3361 gccgggtctc gccggccggc gcgctgcgcg cgaaggaatg ggcgctgctg cggcgcgatc
     3421 cctggctcat ctcgcagacg ctgatgcaat tgctctatct gctgccgccg gcgctgttgc
     3481 tgtggttcag ctatcgcgac cacaatggcg ggacggcgct gctcgtgccg gtgctgatca
     3541 tggccgcggg ccagctcggc ggcggcctcg cctggctcgc catctcgggc gaggacgcac
     3601 ccgacctcgt ggcgacggcg cccgtctccg ccggacgcgc gctcaccgcc aaggtcgagg
     3661 cggtgctggg cggcatcgcg atggtcttcg gtcctttcgc cctggcgctc gccttcgtcg
     3721 cgcccatcga ggcgacggtg gcggtcggcg gcatcctgct cgccgccgcg tcggcgacgg
     3781 cgatccagct cgcgttccgc acgcaagcga agcgcagcca cttccggcgg cgccagatct
     3841 cgtcgcgcat cgccaccttc gccgaggcgc tgtcctcgat cggctgggcc gccaccggcg
     3901 ccttcgccgc cgccggcacc tgggtcgcgg tgatcccggg cgtgctcgcc ctcgccattc
     3961 tcggcggcgc gtggctcatc agcccggcgc ggggctgacg cacgatcgca aaagagaacg
     4021 cagagggcgc ggaggatacg cggagggcgc tgaggaagaa ttttgcgatc gcccagaaac
     4081 ggggattgaa gcagaaaaga atcaccactg aggcggtgag gcagtgagaa gaagaagtta
     4141 tttttggcgc gaagcgccaa tacctcttct cactgtctca ccgcctcacc ggtgattctt
     4201 ttctttttct ctgcgtcctc tgcgtaagcc tctgcgccct ccgcgttcct ctttgttttc
     4261 agagcgactg gaagaaggcg atcattttgc ggcgttgcgc ttcgtcgggg acgcggccgc
     4321 ggccggcgcc gaggtcgtcg gccatgtgat cgggattgtt ggtgccgggg atcaccgcgg
     4381 tgatcgcggg gtggccgagg atgtatttca ggaagacctg ggcccagctt gcggcgtcga
     4441 tgtccttggc ccagtcggga agcggctttc cgcgcacggc gcggaagaga cggccgcgcg
     4501 ctagcgggag atcgacgagc acggccgcgc cgatcgcggc ggcggcgggg agcacgcgct
     4561 gctcggcgtc gcgatcggcg agcgagtagt tgacctcgag gaaatcgggc ttctcgcgct
     4621 tgagcaccgc ctcgaccgca tcgaagtcgc cgccgaagga cgtcgtgatg ccgatatagc
     4681 ggcagagccc ttgcactttc cactcgcgca gcaaagcgag gtcctgcctg ggatcgctga
     4741 cattgtggag ctgcatcaga tcgaccttgt cggtgcgcag gcgctggagc gaggcacgga
     4801 cttcgccggg gcccgtcgcg cggtcatatt cctcgagctt ggtggcgaga aacgtgcggc
     4861 cgcgcagccc ggtctcggcc acgagatcgc cgaccacggc ctcggcgcgg ccgtaagacg
     4921 gcgccgtgtc gatgacgctg ccgccgccat cgatcaagcg gcgaataacc tcggcgcagg
     4981 cacggcgctc cgccgcgtcg ctgccgatgt cgaagacctc cgatgtgccg aggccgatca
     5041 ccggcacagc ctcgcccgaa tgcgggatct ttcgcatgat gagcgcgccg gcgccggtct
     5101 gctccgcagc cccggccggc gagcctccga tccaggcgga agcagcgagc ccggcgcccc
     5161 aggcaaggat gcggcggcgg tcggggtcgc gcgcggcttt gctcatggcg cctctcctgc
     5221 cacgacatcg tcctggccgg tcgcggcggc gcggcgtgcc tcgccgcggc caagcgcgag
     5281 ggcgaggccg gcccagccca gcgcgagcgg gatcgcgatc cccgagatcg ccgccaaggc
     5341 caggccggca tcgcgcaaca cgccgaagac ccagccatag agcgcgtccg agccgcgaaa
     5401 caccaccacg tcgatcacgt tcttggtctt gtatttgtcc tcgcgcggca ccggcgtgaa
     5461 gaggatctgg cggccgagat tcgagaaagc gaaattcgcc gtgcgctgaa tcgtctggaa
     5521 tacgagaacg acggtgacat tcggaaagac ggcaagggcc gcgaagccca ggacgaagac
     5581 caccggttga acggcgagcg cggcggcgac gccgaagagc ttgaccagcg gtccggagac
     5641 gaatgcctgg acgacgatcg tggcgatggc gacgtagaga tcgatcctgg cgaagaactg
     5701 ggtttgcgcc gcggcggttt gcctggcggc ggcgaccaga ttcgcctgct cgaaatagag
     5761 gatggtgccg gcaaaggaga ggaggctgac ccagagcgcg atgcccgcga gatagggcga
     5821 ggcgaagagc tgcgtgatgc cggcaaacca gctcccgccg acgcgcgccg cgccgatcgc
     5881 agcggcctct tgggcgccgg cgacagatcc ggccgctctt tccagacgat gcgcgcagag
     5941 gacggcgagc tcgaggagca ggccggcgat gatgaggaga ttgacgggcc cgagcgcgtc
     6001 ggcgagaccg agcacgatca ccgggccgag gatcgacccg gcggtgccgc ccgcgccgat
     6061 caggccgaag agccgcttgc tctgctcgct gcggaacagg tcggccatga acgaccagaa
     6121 caccgacacc gcgaagagat tgaagacgct gatccagacg aagaagatcc aggccatgac
     6181 gacgggcgcg acgccgagcg tgagcagcag ccagaacagg gcgagattgg cgacgaagaa
     6241 gtgatagacg atgggaatga agcg
//
LOCUS       DASYNS010000494         3306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4496049, whole genome shotgun sequence.
ACCESSION   DASYNS010000494 DASYNS010000000
VERSION     DASYNS010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4496049"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VGP50_15370"
     CDS             <1..294
                     /locus_tag="VGP50_15370"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV8028812.1"
                     /translation="YVGLRRQAALAGIIGFSGALVGADALPSEIRSRPPVLLVHGDAD
                     EVVPFGALEASVRGLQAAGVPVEDVVRPGLGHGIDEEGLRRAAEFLRRIFARP"
     gene            complement(310..945)
                     /locus_tag="VGP50_15375"
     CDS             complement(310..945)
                     /locus_tag="VGP50_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV8028813.1"
                     /translation="MKGLLRALILLSVLSASVLLVLAYSQRGVDQSEPGASDSGRGDT
                     PRATTVGQFTPLDPPRPAPEASFTERDGTPRRLADFRGRFVLLNLWATWCAPCVREMP
                     SLDRLQAKLGSRLAILAVSEDRGGAHVVDPFLEKLGLKALATYLDPKSALSGDLEVTG
                     LPTTFLIDDQGRILGQLEGAAEWDSPRMVRTIERYLQGASVSGVVKTSGVR"
     gene            982..2394
                     /gene="argH"
                     /locus_tag="VGP50_15380"
     CDS             982..2394
                     /gene="argH"
                     /locus_tag="VGP50_15380"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV8028814.1"
                     /translation="MTESSSAGSGRPASAAWGGRFAGGPAEIMQRINVSIAFDKRLYA
                     QDIAGSLAHCDMLVRQKVVSREDGDAIARGLAQIQGEIEAGTFPFRDEHEDIHLNIEA
                     RLGELIGPAAGRLHTARSRNDQVATDFRLWLRDAIDRLDRQMQALQAALIDQAERHAA
                     TIMPGFTHLQTAQPVTFGHHLLAYVEMLGRDRGRLADCRKRLNESPLGAAALAGTSFP
                     IDRAMTAKALGFDRPAANSLDAVSDRDFALEFLAAAAIAGMHLSRFAEEIVLWMSAPY
                     GFIRLSDAFTTGSSIMPQKRNPDAAELVRAKAGRLAGALMALLAVMKGLPLAYSKDMQ
                     EDKLPVFEAADTLELCIAATAGMVLDLTVDAEAMRSAAAGGFSTATDLADWLVRVLAL
                     PFREAHRITGALVKRAELLGSDLARLPLDEMQRIEPRITHAIYDVLTVEKSVASRTSF
                     GGTAPANVAAAAKEARKRFL"
     gene            2403..2561
                     /locus_tag="VGP50_15385"
     CDS             2403..2561
                     /locus_tag="VGP50_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028815.1"
                     /translation="MILAVPSRRRHAALMLALLLAAVLPLAACGKKAAPGPPSGEPNT
                     YPRTYPRE"
     gene            2554..>3306
                     /locus_tag="VGP50_15390"
     CDS             2554..>3306
                     /locus_tag="VGP50_15390"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV8028816.1"
                     /translation="MSELEIEQGAPDFAYHLGELQAEGVALSRIAAEVGTPFYCYSSA
                     TLERNYRDFATAFADRDCGIFYSVKANSNLAVIRTLARLGAGADVVSEGELRRALAAG
                     LPAGKIIFSGVGKSEAELRFAVERGVFQVNVESEPELRLLSSVATSLGKRAPVALRVN
                     PDVDARTHAKIATGKKENKFGIDLAQAPAILRLAATLSGIAPVGLAVHIGSQLTELTP
                     FELAFARVGELIRAARRDGIALGRLDLGGGLGN"
BASE COUNT          491 a         1184 c         1090 g          541 t
ORIGIN      
        1 tatgtcggcc tgcggcgtca ggctgcgctt gcggggatca tcggcttctc gggagcgctg
       61 gtcggcgccg acgcgctgcc gtcggagatc cgctcgcgcc cgcccgtgct gctcgtgcac
      121 ggcgacgccg atgaagtggt accgtttggc gccctcgaag cctcggttcg gggtttgcag
      181 gccgcgggcg ttcccgtcga ggacgtcgtt cgacccggtc tcggccatgg catcgacgaa
      241 gaggggctgc gccgcgccgc cgagttcctg cgtcgcatct tcgcgcgccc ctgaaccgcc
      301 ggtgcccttt cagcggactc ccgatgtctt cacgacgccg gacacgctgg cgccctgcag
      361 ataccgttcg atcgtcctca ccatccttgg cgaatcccac tccgccgcgc cttcgagctg
      421 gccgaggatg cgcccctgat cgtcgatgag gaaggtcgtc ggcagacctg tgacctccag
      481 atccccgctc agcgcgcttt tgggatcgag ataggtcgcc agcgccttga ggccgagctt
      541 ctcgaggaac ggatccacca catgggcgcc gccgcggtcc tcggagacgg cgaggatggc
      601 aagccggctg ccgagcttcg cctgcaggcg gtcaagcgac ggcatctcgc gcacgcaggg
      661 cgcacaccac gtggcccaga gattgagcag cacgaagcgg ccgcggaaat cggcaagccg
      721 gcgcggcgtt ccgtcgcgct cggtgaagct cgcctcgggc gctggtcgtg gcggatcgag
      781 cggggtaaac tggccgaccg tcgtggcgcg tggggtgtcg cccctgccgc tgtcgctggc
      841 gccgggctcg ctctgatcga cacccctctg cgaataggcg agcaccagca gcaccgacgc
      901 cgagaggacc gagagcagga tgagcgcgcg cagaagccct ttcacggccc ttccttcggg
      961 tgacgagcgc cgtcgcagat catgaccgaa tcatcctccg ccggctccgg ccggccggcc
     1021 agcgccgcct ggggcggccg tttcgccggc ggcccggccg agatcatgca gcgcatcaat
     1081 gtgtcgatcg ctttcgacaa acgcctctat gcccaggaca tcgccggctc gctcgcgcat
     1141 tgtgacatgc tggtgcggca gaaggtggtc tcgcgcgagg atggcgacgc gatcgcccgc
     1201 ggtctagcgc aaatccaagg cgagatcgag gccggcacct tcccgttccg cgacgagcat
     1261 gaggacatcc atctcaacat cgaagcgcgg ctcggcgagc tgatcggccc ggcggcgggg
     1321 cggctccata cggcgcgctc gcgcaacgat caggtggcga ccgatttccg cctgtggctg
     1381 cgcgacgcca tcgatcgcct cgaccggcag atgcaggcgc tgcaagcagc gctgatcgat
     1441 caggccgagc gccatgctgc caccatcatg ccgggcttca cccatctcca gacggcgcag
     1501 cccgtgacct tcggccatca tctcctcgcc tatgtggaga tgctgggacg agaccgcgga
     1561 cgcctcgccg attgccggaa acgcctcaac gaatcgccgc tcggcgccgc agcgcttgcc
     1621 ggaacgtctt ttcccatcga ccgtgccatg acggccaagg ccctgggctt tgaccggccg
     1681 gcggccaatt cgctcgacgc cgtctccgac cgcgacttcg cgctcgagtt cctcgccgcc
     1741 gcggccatcg ccggcatgca tctgtcgcgc tttgccgaag agatcgtgct ctggatgagc
     1801 gcgccctatg ggttcatccg cttgagcgac gccttcacga ccggcagctc gatcatgccg
     1861 cagaagcgca atcccgacgc ggccgagctg gtgcgcgcca aggcgggccg cctcgccggc
     1921 gcgctcatgg ccttgctggc cgtgatgaaa ggcctgccgc tcgcctacag caaggacatg
     1981 caggaggaca agctgcccgt gttcgaggcg gcggatacgc tcgagctctg catcgccgcg
     2041 accgcgggca tggtgctcga tctcacggtc gatgccgaag ccatgcgcag cgccgccgcc
     2101 ggcggcttca gcaccgcgac cgatctcgcc gactggctgg tgcgcgtgct ggcgctcccc
     2161 ttccgtgagg cgcatcgcat caccggcgcg ctggtgaagc gcgccgagtt gctgggcagc
     2221 gatctggcgc gcctgccgct cgacgagatg caacggatcg agccgcgcat tacgcacgcc
     2281 atctacgacg tcctcaccgt cgagaaatcg gtggcgagcc gcaccagctt cggcggcacg
     2341 gcgccagcca atgtcgccgc cgccgcgaaa gaggcgcgca agcgcttcct gtgaggaatc
     2401 ccatgatcct tgccgtcccc tcccgccggc gccacgctgc gctgatgctc gctctcctgc
     2461 tcgctgccgt gctgccgctc gcggcctgcg gcaagaaagc ggcgcctggc ccgccatccg
     2521 gcgagcccaa cacctacccc cggacctatc cccgtgagtg agcttgagat cgagcaaggc
     2581 gcgccggact tcgcctacca cctgggcgag cttcaggccg aaggcgtggc cctttcgcgc
     2641 atcgccgccg aagtcggcac gcccttctat tgctactcga gcgccacgct cgagcggaac
     2701 taccgcgatt tcgctactgc cttcgccgat cgcgactgcg gcatcttcta ttcggtcaag
     2761 gcgaattcca accttgcggt gatccgcacg ctggcgcggc tgggagccgg tgccgatgtg
     2821 gtctccgaag gcgagcttcg ccgcgccctg gccgcgggcc tgccggccgg caagatcatc
     2881 ttctcgggcg tgggcaagag cgaggccgag cttcgtttcg cggtcgagcg tggcgtcttt
     2941 caggtcaatg tcgaatcaga gccggagctg cggctgctga gcagcgttgc gacctccctc
     3001 gggaagcgcg cgcccgtcgc gctgcgcgtc aaccccgatg tcgatgcccg cactcacgcc
     3061 aagatcgcca ccggcaagaa ggagaacaag ttcggcatcg atctcgcgca ggcgccggcc
     3121 atccttaggc tcgcggcgac gctctccggc atcgctcccg tcgggctcgc cgtgcatatc
     3181 ggctcgcagc tcacggagct gacgcctttc gagctggcct tcgcgcgggt tggcgagttg
     3241 atccgtgcgg cgcgccgcga cggcatcgcc cttggccgcc tcgacctcgg cggcgggctc
     3301 ggcaac
//
LOCUS       DASYNS010000495         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5271071, whole genome shotgun sequence.
ACCESSION   DASYNS010000495 DASYNS010000000
VERSION     DASYNS010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5271071"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1888)
                     /locus_tag="VGP50_15395"
     CDS             complement(2..1888)
                     /locus_tag="VGP50_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028817.1"
                     /translation="MAEEARIGDYPAAITARPEGAVAARRGFFGRIGRPLVCLLALAT
                     AACNLFPHTDGTAKDVPGGLETIARHYDIQPLDPQSKVPLVKQAAEQLEETDPLRRLR
                     GTTYRLSYGNHLAADWIIQTPNVWGKRAAAVRYVPSDCKHCDPDFRLHACAAASRCGG
                     RPCVALAASAAAPGERPRKFCVGHSDPVIDRFYGLIVSAQQSVDITMLAPAADLRVLA
                     ALRNGITWLAHSGRAVTIRAIVGDYPPAGFDAAAFLHELVRDAAAVPTSRLRVYAAAT
                     RSCDVNASCGGISWNHAKIVAVDGQRAIVGGHNLWTPDYLVAAPVHDISMAVEGPAAY
                     DAHRFADALWRSVCALPRSDGVNATFVSLGPASNGGEDCPRHIALPEREEGEGGTPIL
                     AVGRLASGIVKGFADQSLIARDLMLGAARRTIRMVQQDVGFALLSGQVDRSWPESALG
                     DIAELLGKDGDVYMILSNLGAAGPIGTYSNGIPLEAVADKIKDVVKQRTGLVEPQLSS
                     LLCQHLHLAPLRFGPDETWPNDVPIGTHAKFWMVDDRVFYIGSENLYPADLQEFGYIV
                     EDAAAVAQLHRDYWDKAWQWSRPAAISGDDAPRCIFQDKTSQSGSRSSGISASSTSSS
                     PRKL"
     gene            complement(1953..>2166)
                     /locus_tag="VGP50_15400"
     CDS             complement(1953..>2166)
                     /locus_tag="VGP50_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028818.1"
                     /translation="AGSYADPGAPNDPLHRPLGPPIMLHGALELGYHIGAQHSLSLSL
                     DQGRAPAAGLGGETSDNLRLRYGLRF"
BASE COUNT          365 a          708 c          765 g          328 t
ORIGIN      
        1 cctagagctt tcgcggggac gacgatgtcg aggaagcgct gattccactt gatcgagatc
       61 cgctctggct cgtcttgtcc tgaaagatgc agcgcggcgc gtcgtcgccc gagatcgcag
      121 ccgggcgcga ccattgccac gctttgtccc agtagtcgcg gtgaagctgc gccacggccg
      181 cggcgtcctc gacgatgtag ccgaactcct gcaggtcggc cggatagaga ttctccgagc
      241 cgatgtagaa gacgcgatca tccaccatcc agaatttggc atgcgtgccg atcggcacgt
      301 cgttgggcca ggtctcgtcc gggccgaaac gcagcggcgc caggtggaga tgctggcaga
      361 gcagcgacga gagctgcggc tcgacgagac ccgtccgctg cttgaccaca tccttgatct
      421 tgtccgccac cgcctcgagg ggaatgccgt tgctgtaggt gccgatgggc ccggcggcgc
      481 cgagattcga gaggatcatg tagacgtcgc cgtccttgcc gagcagctcc gcgatgtcgc
      541 ccaaggcgct ttccggccag gagcggtcga cctgcccgct cagcagcgcg aagccgacat
      601 cctgctgcac catgcggatg gtccgccgcg ccgcgcccag catgaggtcg cgggcgatca
      661 gcgactgatc ggcgaaaccc ttgacgatgc cggaggcgag gcggccgaca gcgaggatcg
      721 gagtgccgcc ctccccttct tcgcgctcgg gcaacgcgat atggcgggga caatcctcgc
      781 cgccgttgct cgcgggtccc agcgatacga aagtcgcgtt gacgccgtcg ctgcgcggca
      841 gcgcacacac cgagcgccag agcgcgtcgg cgaagcggtg cgcgtcatag gccgccggtc
      901 cctcgaccgc catcgagatg tcatggaccg gcgctgcgac gagataatcc ggcgtccaga
      961 gattatgccc gccgacgatc gcgcgctggc cgtcgacggc gacgatcttt gcgtggttcc
     1021 acgagatccc gccgcagctc gcgttgacgt cgcaagagcg cgtggccgcg gcatagacgc
     1081 gaagccgcga cgtcggcacc gccgccgcgt cgcgcacgag ctcgtgaagg aatgcggcgg
     1141 cgtcgaatcc ggccggcgga tagtcgccga cgatggcccg gatggtcacg gcacggccgc
     1201 tatgcgcgag ccaggtgatg ccgttccgca gcgccgcgag gacgcgcaga tcggcggccg
     1261 gcgccagcat ggtgatgtcg accgattgct gtgccgaaac gatgagcccg tagaaacggt
     1321 cgatcaccgg gtcggaatga ccgacgcaga acttccgcgg ccgttcgccc ggcgccgcag
     1381 cggaggcggc gagcgcgacg caaggacggc cgccgcagcg ggaggccgcg gcacaagcgt
     1441 gaaggcggaa atccggatcg cagtgcttgc agtcgctcgg gacatatctc accgctgccg
     1501 cccgcttccc ccagacattg ggtgtctgga tgatccagtc cgcggccaaa tggttgccgt
     1561 agctcaagcg ataggtcgtg ccgcgcaggc ggcgcagtgg atccgtctcc tcgagctgct
     1621 ccgcggcctg cttgacgagg ggcaccttcg attgcggatc aagcggctgg atgtcgtaat
     1681 gccgggcgat cgtctcgagg ccgcccggga cgtccttcgc cgtcccgtcg gtgtgcggaa
     1741 agaggttgca agccgcggta gccagcgcca ggaggcagac cagaggccgg ccgatcctgc
     1801 caaagaaccc gcggcgggcg gcgacggcac cctcggggcg tgccgttatc gccgccgggt
     1861 agtcgccgat gcgcgcttcc tcagccacag attaggaaca caggaggatc ccgggtgatc
     1921 cattcccgtt acggcgccga aatttgacgc ggtcagaaac gcaggccgta gcggaggcgg
     1981 agattgtcgc tggtctcgcc gcccaaaccg gcggcgggcg cgcggccctg gtcgagcgac
     2041 agcgacaggc tgtgctgcgc gccgatgtga tagccgagct cgagggcgcc atggagcatg
     2101 atcggcgggc cgagcggccg gtgcagcgga tcattggggg cgcctggatc ggcatagctc
     2161 ccggca
//
LOCUS       DASYNS010000496         6209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1841211, whole genome shotgun sequence.
ACCESSION   DASYNS010000496 DASYNS010000000
VERSION     DASYNS010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841211"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VGP50_15405"
     CDS             <1..595
                     /locus_tag="VGP50_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028819.1"
                     /translation="HDKLVVVDFNGQNPTVFTGSSNLAAGGETANGDSLAMIEDADVA
                     GMYAIEAVAMFDHYHFRKAMQQATKPQPLTLWYPGKANAPLPWWKLYYDPTKIQLRDR
                     CLFAGVPLPAGVATTKNVDWSAVDAAGGAAAKTPRAKAATKPAAKRAATRGKAGRKRP
                     PAKRATPKRRPAPKKAKRKAAAPRSAERKATPSRKRR"
     gene            930..3665
                     /locus_tag="VGP50_15410"
     CDS             930..3665
                     /locus_tag="VGP50_15410"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV8028820.1"
                     /translation="MAAARKRRAKPAVERAPAPLGWLSDDALEIERRHWRGKTEIGAI
                     ETLEPGQPFFGSFRVSSAGGSGYDVEIRSLDRRINSCGCADHRVNGLGTCKHIEGALA
                     RLRRRGKRACAAAEQLGSMRVELFLDRADGVRPALLWPTDRRAVAAADAVLAPFLAAD
                     GGLADASPEGVDAVLRAIARADAATQMQIRVSRGLASWIADARRRAERLAARERFLAE
                     AAAGRQTLEVLRHELLPYQREGMLHLAFGERALLADDMGLGKTAQAIAAAELLRRLRG
                     IARVLVVCPASLKGEWQEQIARFSGAATVLVAGSRAARLGQYGSPGFFTVVNYEQVVA
                     DAPDINRLVKPEIVILDEAQRIKNWQTKTARLVKSLASPYAFVLTGTPIENRIDEIYS
                     IVQYLDPELLGPLFRFNRDFYALDERGRPSGYKNLDELMRRLKPVMLRRRKCDVEKEL
                     PGRTVTNYLVAMNDEQRQRYDEYKAQAARLVQLAQRRPLAPQEFERLQMLLACMRMLC
                     DTPFILDPACRVSPKIEELERLLAELLAEPERKVIVFSEWERMLVLVRELALDMGIEI
                     AWHTGSVPQERRRAEIDRFKHDPGCRLFLSTDSGSVGLNLQAASAVINLDLPWNPAKL
                     EQRIARAWRKNQRRIVDVINLVTEDSIEHAMLHVLAQKQALADGVLDGAGDLKAMPLP
                     SGRRAFIERMSALMAPPVAAPPEPVPALSAPSAPSLREELAEHHGEALLLIETHRSAD
                     GTETVLAVLDGDAAAAERQRRADRGAPRVEVLDRATYETIQRLSSAGVLHAASGQREE
                     LFRSARLPASTGERERQRIAKGAELLSGAERKLRMALLLAQGGFAAEAMPALSECLTL
                     AADARKLMAGEEASEPAAAAPVGAASLGAVDDIAGSIERVLGDVRRSLSAARIAA"
     gene            complement(3696..4985)
                     /gene="oxlT"
                     /locus_tag="VGP50_15415"
     CDS             complement(3696..4985)
                     /gene="oxlT"
                     /locus_tag="VGP50_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEV8028821.1"
                     /translation="MSVSAVAAPGVLRSRWLQLTLGVIAMMAISSPQYTWTLFVGPLN
                     QKLGTTLAEIQFTASLLIILQTFVSPVQGYLVERFGPRNLIAAGAFLSGAGWVLAAQA
                     QSLWMLYLTYGVIGGFGTGIIYVGVIGLMVRWFPDRRGLATGLAAAGYGFGAFFTTIP
                     ITNMIAASGYQRTLLVWGLIQGVVGILAAVGLRMPSSGYLPAGYDPETARTEMQTKRS
                     YTPGEMLRNPIFYLLFVMMAMMSTGGLMVVLNIAPFAKQYGVANVGIATLPFFGAVTA
                     LGLSLTLSRVTNGLTRPFFGWISDHIGREATMALAFALEAAAILVLFAYIDRPVLFVV
                     LTGLVFFGWGEIFSLFPSTLTDTFGAEHAMTNYGFLYIAQGVGSILGGPAAAKLHDAT
                     GSWLPVFILVACLDVLTAVLAITVLRRLRQRHLRIAG"
     gene            complement(5048..5170)
                     /locus_tag="VGP50_15420"
     CDS             complement(5048..5170)
                     /locus_tag="VGP50_15420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028822.1"
                     /translation="MAMDQELERHLETWRGFARLLRWSVSAIGVVLIVLALWLL"
     gene            complement(5183..>6209)
                     /locus_tag="VGP50_15425"
     CDS             complement(5183..>6209)
                     /locus_tag="VGP50_15425"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEV8028823.1"
                     /translation="LAERRRPVGDAAAPRAAVVAVAVLLAAQAGYAAKELRHPSLIDA
                     ATTTLAAGEVLRGGASPYATALDPEGMAVVGDQRFGGYKYLPMTIAAYLPLGAPFGQR
                     GIVLTNLLLQLAMAWLILRLGIRMAGASAAWLGLLLYLSLPMVPFQLFAKGATDLVAV
                     LPLLAALLLFERRPGLAGLCLGLALAAKPLPGILLLPCCLPEQRSARIAYATGVAAGL
                     LPVLPFLVWSSAALYDNVVQFNLMRPADSTSWLAAAPPLAGAVAHAVLALLYAGVAVH
                     IWLRPPTLLRRTGAAVGLTLAAILAAPAAHHNYQLWWLPLVAPLLAAALAPPTAPAVY
                     EAPKVAA"
BASE COUNT         1100 a         2096 c         2184 g          829 t
ORIGIN      
        1 ccacgacaag ctggtcgtcg tcgacttcaa cggccagaat cccaccgtct tcacgggatc
       61 gtcgaatctc gcggccggtg gcgagaccgc caatggcgac agcctcgcca tgatcgagga
      121 cgcggacgtc gccggcatgt acgcgatcga ggcggtcgcg atgttcgatc actaccactt
      181 ccgcaaggcg atgcagcagg cgacgaagcc gcagccgctg acgctctggt atcccggcaa
      241 ggccaatgcg ccactaccct ggtggaagct ctattacgat ccgaccaaga tccagttgcg
      301 cgatcgctgc ctctttgccg gcgtgccgct gccggcgggc gttgccacga cgaagaacgt
      361 cgattggagc gcggtcgatg cggcgggcgg cgccgcggca aagacgccgc gcgccaaggc
      421 cgcgacgaag ccggcggcga aacgggccgc gacccgcggc aaggcagggc gcaagcgtcc
      481 gccggcgaag agggcgacgc ccaagcgtag gcccgcgccg aagaaggcca agcgcaaggc
      541 cgccgcaccg cgaagtgccg agcgcaaagc gacgccgtct cgcaagcgcc gctagtaacc
      601 gcgccaggct ccgctgagcg gaggcgcacc accgcagcca gcatcttctc acgcgaccgc
      661 ccggcatcgt cgggaaccga gccaccgcga gtcgccgcga gactccggcc gaagcgcggc
      721 cgaggcgcat ggcggcaccg gatatcgccg gaggccgaac ggctcgcacg cgtcatcgag
      781 cagaggtccc gatcgcggtt ccccatcgat atgttgcgcg tggcgccaag cttcgtccaa
      841 tatgttgtgt gcgcacggtt agggtgtcga gggggactat gctcgattcg cggcagctca
      901 gagcatcgaa tggccggaat gacgtgaggt tggcagccgc gcgtaagcgt cgcgcgaagc
      961 cggcggtcga gcgcgcgccg gcgcccctcg gctggctctc cgatgacgcc ctggagatcg
     1021 agcgacggca ctggcgcggc aagaccgaga tcggcgcgat cgagacgctg gagcccggac
     1081 agcccttctt cggcagcttc cgcgtgagct cagccggcgg cagcggctac gatgtcgaga
     1141 tccgctcgct cgaccgccgg atcaattcct gcggctgcgc cgaccaccgc gtcaacgggc
     1201 tcggcacttg caagcacatc gagggcgcgc tggcgcggtt gcgccggcgc ggcaagcgcg
     1261 cctgcgcggc ggcggagcag ctcgggagca tgcgagtcga gcttttcctc gatcgtgcgg
     1321 atggcgtccg cccggcgttg ctctggccca cggaccggcg cgcggtcgcc gccgccgatg
     1381 cggtcctggc acccttcctc gccgccgatg gcgggcttgc ggatgcatcg cccgaaggcg
     1441 tcgatgccgt gctgcgcgcc atcgcccgcg ccgatgcagc gacgcagatg cagatccgcg
     1501 tctcgcgcgg gctcgcgtcc tggatcgccg acgcgcggcg gcgggcggag cgtttggcgg
     1561 cgcgcgagcg ctttctcgcc gaggccgccg ccggacggca gaccctcgaa gtgctgcgcc
     1621 acgagctgct gccctatcag cgcgagggca tgctgcatct cgccttcggc gagcgcgcgc
     1681 tcctcgccga tgacatgggg ctcggcaaga ccgcgcaggc gatcgccgcc gccgagcttc
     1741 tgcgccgcct acgcggcatc gcccgcgtgc tggtggtgtg cccggcgtcg ctcaagggcg
     1801 aatggcagga gcagatcgcc cgcttcagcg gcgcagccac agtgctggtc gccggcagcc
     1861 gcgccgcgcg gctcgggcaa tacgggtcgc cgggtttctt cacggtcgtg aactatgagc
     1921 aggtcgtcgc cgacgctccc gacatcaatc gcctcgtgaa gcccgaaatc gtcatactcg
     1981 acgaggcgca gcgcatcaag aactggcaga ccaagacggc gcgcctggtg aagtcgctgg
     2041 cgtcgcccta tgccttcgtg ctcacgggca cgccgatcga gaaccggatc gacgagatct
     2101 actcgatcgt gcaatacctc gatccggagc tgctggggcc gctcttccgc ttcaaccgcg
     2161 atttctacgc gctcgacgag cgcggtcggc catcgggcta caagaacctc gacgagctca
     2221 tgcgccggct gaagccggtc atgctccgcc gccgcaaatg cgacgtcgag aaggagctgc
     2281 caggacgcac ggtcaccaac tatctggtcg ccatgaacga cgagcagcgt cagcgctacg
     2341 acgagtacaa ggcgcaggcc gcgcgcctcg tccagctcgc gcagcgccgg ccgctcgcgc
     2401 cgcaggaatt cgagcgcctg caaatgctgc tcgcgtgcat gcgcatgctg tgcgatacgc
     2461 ccttcatcct cgatcccgcc tgccgggtga gcccgaagat cgaggagctc gagcggctgc
     2521 tcgccgagct gcttgccgag cccgagcgca aggtgatcgt gttctcggaa tgggagcgta
     2581 tgctcgtcct ggtgcgcgag ctggcgctcg atatgggcat cgagattgcc tggcataccg
     2641 gctccgtgcc gcaagagcgc cgccgcgccg agatcgaccg cttcaagcat gatcctggct
     2701 gccggctgtt cctgtcgacc gacagcggca gcgttggcct caatctccaa gcggcgagcg
     2761 ccgtgatcaa tctcgatctg ccctggaacc ccgccaagct cgagcaacgc atcgcacgcg
     2821 cctggcgcaa aaaccagcgg cgcatcgtcg acgtgatcaa cctcgtcacc gaggacagca
     2881 tagaacacgc gatgctccat gtgctggcgc agaagcaggc gctggccgac ggcgtgctcg
     2941 acggcgcagg cgatctcaaa gcgatgccgt tgccgtccgg ccggcgcgct ttcatcgagc
     3001 ggatgagcgc gctgatggcg ccgcccgtcg cggcgccgcc ggaaccggtc ccggcgctga
     3061 gcgcgccatc cgcgccgagc ctgcgcgagg agctggccga gcaccatggc gaggcgctcc
     3121 tgctcatcga aacccatcgc agtgccgacg gcaccgaaac cgtgctcgcc gtgctcgacg
     3181 gcgatgccgc cgcagctgaa cgacagcgcc gcgccgatcg aggcgctccg cgggtcgaag
     3241 tgctcgaccg cgcgacctac gagacgatcc agcgtttgtc cagcgcgggc gtgctgcacg
     3301 cggcgtccgg gcagcgcgag gagctgtttc ggtcggcgcg gcttcccgcg tcaacgggcg
     3361 agcgcgagcg tcagcgcatc gccaagggcg cggaactcct cagcggcgcc gagcgcaaac
     3421 tgcggatggc gctgctcctc gcgcaaggcg gtttcgccgc cgaggcgatg ccggcgctga
     3481 gtgagtgcct gacgctcgcc gcagatgcgc gcaagctcat ggctggcgaa gaagcgtccg
     3541 agccggccgc cgcggcgccg gtcggagcgg catcgctcgg cgccgtcgat gatatcgccg
     3601 gttcgatcga gcgcgtgctc ggcgacgtgc ggcgcagcct gagcgcggcc cggatcgctg
     3661 cttgatcgcg cttggggtgg cggtcgtgcg aaccgctagc ccgcgatgcg caggtgccgt
     3721 tggcgaaggc gccgcaggac cgtgatcgcc agcaccgccg tcagcacatc gagacaagcg
     3781 acgaggatga acacggggag ccagctcccg gtggcgtcat ggagcttggc ggccgccggg
     3841 ccgccgagga tcgagccgac gccttgcgcg atgtagagga agccgtaatt cgtcatcgca
     3901 tgttccgcgc cgaaggtatc ggtgagtgtc gacgggaaga gcgagaagat ctcgccccag
     3961 ccgaagaaca cgagaccggt caagaccacg aacaggacgg gacggtcgat ataggcgaag
     4021 agcacgagga tggccgcggc ctcgagcgcg aaggcgagcg ccatggtcgc ctcgcggccg
     4081 atatggtccg agatccagcc aaagaatggc cgcgtcagcc cgttggtcac gcgcgacagc
     4141 gtgagcgata ggcccagcgc cgtgaccgcg ccgaaaaacg gcagggtcgc gatcccgacg
     4201 ttggcgacgc catactgctt ggcgaaaggc gcgatgttga gcacgaccat caacccgccg
     4261 gtcgacatca tggccatcat gacgaacagc agatagaaga tcggattgcg cagcatctcg
     4321 ccgggcgtat agctgcgctt ggtctgcatc tccgtccggg cggtctcggg gtcgtagccg
     4381 gcgggcagat agccgctcga cggcatgcgc aatcccaccg ccgcgaggat gccgacgacg
     4441 ccctggatga ggccccagac cagcagcgtg cgctgatatc ccgaagcggc aatcatgttg
     4501 gtgattggaa tcgtcgtgaa gaaggcgccg aagccgtagc cggccgcggc gaggcccgtg
     4561 gcgagcccgc ggcggtcggg aaaccagcgc accatcaggc cgatgacgcc gacatagatg
     4621 atgccggtcc cgaagccgcc gatgacgcca taggtgagat agagcatcca caggctctgg
     4681 gcctgggccg ccagcaccca gccggcgccg gacagaaacg cacctgctgc gatgagattg
     4741 cgcgggccga agcgctccac gagatagccc tggacgggcg agacgaaggt ctgaaggatg
     4801 atgaggagcg acgccgtgaa ctggatctcc gccagcgtcg tgccgagctt ctggttcaac
     4861 gggccgacga acagcgtcca ggtgtattgc gggctcgaga tcgccatcat ggcgatgacg
     4921 ccgagcgtga gctgcagcca gcggctgcgc aagacgccgg gcgcggcaac ggcagagacg
     4981 gacatgcttg accccagact ggcgagcgct taccggacgg ctgagccggc gccgttcagt
     5041 attgccgcta cagcagccag agggcgagga caatcagcac gacgccaatg gcggagacgc
     5101 tccagcggag caagcgggca aaacctcgcc acgtctccaa atggcgctcc agctcctgat
     5161 ccatggccat ccgatattcc ccctaagccg cgaccttcgg cgcttcgtat actgccggcg
     5221 cggttggcgg cgcaagcgcg gcagcgagga ggggagcgac aagcggtagc caccagagct
     5281 ggtaattgtg atgcgccgcc ggcgcggcca ggatcgccgc cagcgtgagc ccgacggccg
     5341 cgccggtgcg gcgcaggaga gtgggcggcc ggagccagat atgaacggcg acgccggcgt
     5401 agagcagggc gagcacggca tgcgcgaccg cgccggccag cggcggcgcc gccgcgagcc
     5461 agctcgtcga atcggccgga cgcatcaggt tgaattgcac gacgttgtcg tagagcgcgg
     5521 ccgacgacca gacgaggaac ggcagcaccg gcagcagccc ggcggcgaca cccgtggcgt
     5581 aggcgatgcg ggcggatcgc tgttccggca gacagcacgg caagagcaaa atccccggca
     5641 gaggcttggc ggcgagcgcc aagccgaggc acagcccggc gaggcccgga cgacgctcga
     5701 agaggagcag cgccgccagc aatggcagca cggctaccag atcggtcgcg cccttcgcga
     5761 agagctggaa cggtaccatt ggcaggctca gatagaggag aagcccgagc cacgccgcgc
     5821 tcgcgccggc catgcggatg ccgagccgga ggatcagcca cgccatggcg agctgcagca
     5881 gcaggttggt gaggacgatg ccgcgctggc cgaagggcgc gcccaacggc aaataggcgg
     5941 cgatcgtcat cggcagatac ttgtagccgc caaagcgctg atccccgacg acggccatgc
     6001 cttcaggatc gagcgccgtc gcataggggc tcgcgccgcc gcgcagcacc tcgccggcgg
     6061 cgagcgtcgt ggtggcggca tcgatcagcg acggatggcg gagttccttt gccgcatagc
     6121 cggcctgagc cgccaacagg acggcaaccg cgaccaccgc ggcccgcggc gcggcggcgt
     6181 cgcccacggg gcgccggcgc tcggcaagc
//
LOCUS       DASYNS010000497         3476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1996765, whole genome shotgun sequence.
ACCESSION   DASYNS010000497 DASYNS010000000
VERSION     DASYNS010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996765"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VGP50_15430"
     CDS             complement(<1..432)
                     /locus_tag="VGP50_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HEV8028824.1"
                     /translation="MTPSLLSRRSMAIAALVCALVLFVAINIIAERVLKGARLDLTHE
                     RLFTLSQGSKNTLAHIDEPITLRFYYSPRLGDEAPSYGVYAQRVREMLEEYAALAKGK
                     INLEILNPEPFSPAEDRAVAFGLQGVPIDQGGEQVYFGLAAS"
     gene            complement(446..1180)
                     /locus_tag="VGP50_15435"
     CDS             complement(446..1180)
                     /locus_tag="VGP50_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV8028825.1"
                     /translation="MRSTFVIFRRELLSYFATPLAYVFIVIFLAMAGAFAFFLGNFFE
                     RGQADLQPFFNFHPWLYLVLIPALAMRLWAEERRSGTIELFLTLPISMTEAVLGKFLA
                     AWAFAGIALVLTFPFWITVNYLGNPDNGVILASYIASWLMAGAFLAIGSALSALTKNQ
                     VIAFVVTAAVCFLFTVSGSPLVLGFLTAWAPDGLVRAVASFSFLSHFNAIQRGVIDAR
                     DAVFFLSVITAFLFANAVLVELKKAD"
     gene            complement(1191..2135)
                     /locus_tag="VGP50_15440"
     CDS             complement(1191..2135)
                     /locus_tag="VGP50_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028826.1"
                     /translation="MATDPMISVEHLSRRFGSMTAVDDVTFAVARGEVLGFLGPNGAG
                     KSTTMKMITGFLAPTSGTAVIGGHDILADPIGAKRQTGYLPEGAPAYPDMTPAGFLNF
                     IAHVRGFGGKEAKRRITVAVERTALGSVLDQPIDTLSKGFKRRVGLAQALLHDPPVLI
                     LDEPTDGLDPNQKHEVRRLIAGMPEKAIVISTHILEEVDAVCTRAVIIADGRIVADGT
                     PGTLAARSRHHNAVRIALARDRRDAAAALRRLPSVIEIEELEDGEGPALMVFARDGSS
                     IIAAVAEAAHLGGWPVTAMRVERGRLDDVFRAITLPPP"
     gene            complement(2276..2872)
                     /locus_tag="VGP50_15445"
     CDS             complement(2276..2872)
                     /locus_tag="VGP50_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV8028827.1"
                     /translation="MASNFTRRSFARILAAGLTLTAAVADAEGRKGGGMSAYDFDFVS
                     IDGAKMPLAQYRGRPLLVVNTASYCGFTPQYEDLEAVWKRYRDRGLVVIGVPSNDFGQ
                     QEPGTAPEIKEFCESRYDVDFPLTDKEDVVGPDAHPFFRWIADELGDAGAPRWNFHKY
                     LVGPDGMLAGAWPSTVRPTDEAVISAIEKLLPPPKAGL"
     gene            complement(2895..3083)
                     /locus_tag="VGP50_15450"
     CDS             complement(2895..3083)
                     /locus_tag="VGP50_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028828.1"
                     /translation="MAEEKDGLPAKARRKLVESEERWAREGRLPTGRAGRSEQQRLPP
                     GQRKVSGRAPMDPARGAL"
     gene            3237..>3476
                     /locus_tag="VGP50_15455"
     CDS             3237..>3476
                     /locus_tag="VGP50_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009539215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028829.1"
                     /translation="MSEALLIALIKGIAVGIVIALPAGPVGVLCVRRTLFEGPVYGFV
                     SGLGAATADTLFAVVAGFGVTIVRDFMLRFQDWFGA"
BASE COUNT          559 a         1130 c         1213 g          574 t
ORIGIN      
        1 gctggcggcg agcccgaaat agacctgctc tccgccctgg tcgatgggca cgccctggag
       61 gccgaaagcg acggcacgat cctcggccgg cgagaagggc tcgggattga ggatctcgag
      121 gttgatcttg cccttggcga gggccgcgta ttcttcgagc atctcgcgca cgcgctgcgc
      181 atagacgcca taggagggcg cctcgtcgcc gagccgcggc gagtagtaga aacgcaaggt
      241 gatcggctcg tcgatatgtg ccagcgtatt cttcgatccc tgcgagagcg tgaagaggcg
      301 ttcatgcgtc aggtcgagcc gcgcgccttt cagcacgcgc tcggcaatga tgttgatggc
      361 cacgaagagc acgagcgcgc agaccagcgc cgcgatggcc atgctgcggc gggagagcag
      421 agagggcgtc atcgcttgct cctcctcaat ccgctttctt gagctcgacg agcacggcgt
      481 tggcgaaaag gaatgccgtg atcaccgata ggaagaagac ggcgtcgcgc gcgtcgatga
      541 cgccgcgctg gatcgcgttg aagtggctga ggaagctgaa cgacgcgacc gcccgcacca
      601 gcccgtcggg cgcccaggcc gtgaggaagc cgagcacgag cggcgagccg ctgacggtga
      661 agaggaagca caccgccgcc gtgacgacga acgcgatcac ctggttcttg gtgagcgccg
      721 agagagcgga gccgatggcg aggaaggcgc cggccatgag ccagctcgcg atgtagctgg
      781 cgaggatcac gccattgtcg ggattgccca ggtagttgac cgtgatccag aagggaaagg
      841 tgaggacgag cgcgatgccg gcgaacgccc aggccgcgag gaacttgccc agcaccgcct
      901 ctgtcatcga gatcggcagc gtgaggaaga gctcgatggt gccgctccgc ctctcctcgg
      961 cccagagccg catggcgagc gccggaatga gaacgagata aagccagggg tggaaattga
     1021 agaagggctg caggtcggct tggccgcgct cgaagaaatt gccgaggaag aaggcgaagg
     1081 cgccggccat ggcgaggaag atgacgatga acacataggc gagcggcgtc gcgaaatagc
     1141 tcagaagctc gcggcggaag atcacgaagg tgctgcgcat gggcggagat ctatggtggc
     1201 ggaagcgtga tggcgcggaa gacgtcgtcg agccggccgc gctcgacgcg catggccgtc
     1261 accggccagc cgccgagatg cgcggcctcc gccacggcgg cgatgatcga ggagccgtcg
     1321 cgcgcgaaga ccatgagggc gggtccttcg ccgtcctcga gctcttcgat ctcgatcacg
     1381 ctgggaaggc gccgcagcgc cgccgctgcg tcccggcgat cccgcgccag cgcgatccgc
     1441 acggcgttgt gatggcgcga gcgggcggcg agcgtgcccg gcgtaccatc ggccacgatc
     1501 ctgccgtcgg caatgatgac ggcgcgggtg caaaccgcgt cgacctcctc gaggatgtga
     1561 gtggagatga cgatcgcctt ctccggcatg ccggcgatga gccgccgcac ctcgtgcttc
     1621 tggttgggat cgagcccgtc ggtcggctcg tcgaggatca gcacgggggg atcgtgcagc
     1681 agcgcctggg ccaggccgac gcggcgcttg aaccctttgg agagggtgtc gatgggctgg
     1741 tcgagcacgc tgccgagggc cgtgcgctcg accgcgacgg tgatgcggcg cttggcctcc
     1801 ttgccgccga agccccgcac atgggcgatg aaattgagga agcccgccgg cgtcatgtcg
     1861 ggataggcgg gggcgccctc gggaaggtag ccggtctgcc gcttggcgcc gatcgggtcg
     1921 gcgaggatgt catggccgcc gatgacggcc gtaccgctgg tgggcgccag gaagccggtg
     1981 atcatcttca tggtggtgga cttgccggcg ccgttcgggc cgaggaagcc cagcacctcg
     2041 ccgcgcgcca cggcgaaggt cacgtcgtcc accgccgtca tagagccaaa gcgccgactc
     2101 aggtgctcga ccgatatcat cgggtccgtc gccatcgccc ccaccgtctc ctgcgctccg
     2161 ccatgtgccg cattcattgc ccggaatcaa gcgaatgcgg cggccggatg cagctaaatt
     2221 cggtgtcatc tgttggccga cggcgccggc ccgcggagaa ccggccggcg cgaggctaaa
     2281 ggccggcctt gggcggcggc agcagcttct cgatcgcgga aatcaccgct tcgtcggtcg
     2341 ggcgcacggt cgagggccag gcgcccgcga gcatgccgtc ggggcccacg aggtatttgt
     2401 ggaagttcca gcgcggcgcg ccggcgtcgc ccagctcgtc ggcaatccag cgaaagaagg
     2461 gatgggcgtc gggcccgact acgtcctctt tatccgtgag cggaaagtcg acgtcgtagc
     2521 ggctctcgca gaactccttg atctcgggtg ccgttcccgg ttcctgctgg ccgaaatcgt
     2581 tggaggggac gccgatcacg acgaggccgc ggtcgcgata gcgcttccac accgcctcga
     2641 ggtcctcgta ttggggcgtg aagccgcagt atgacgcggt gttgaccacg agcagcggcc
     2701 gtccgcggta ctgggcgagc ggcatcttcg cgccgtcgat gctgacgaaa tcgaaatcat
     2761 aggcgctcat accgcctcct ttccggccct ccgcgtcggc caccgcggcc gtgagcgtga
     2821 ggcccgccgc caggatccgt gcgaagctcc gtcgtgtgaa gttgctcgcc attccgtttc
     2881 tccgctccgc tcagctacag cgctcctcgc gccggatcca ttggcgctct tccagaaacc
     2941 ttgcgctggc ccggcggcag gcgctgctgc tcggaacgcc ctgcacgccc ggtcggaagc
     3001 cggccctcgc gcgcccagcg ctcctcgctc tcgaccagtt tgcgccgcgc cttcgccggg
     3061 agcccgtctt tttcctcggc catccgcctc ctccgcgacc gcacataaca tagtgtgcgc
     3121 ggccctgcgc gggagcgatt ttttcgccgg cgggcgtttt tccccggttg cgctcattgc
     3181 catgctcgcg gagactggcg cgccgcgttc tcccgccccg catttcgttc ctcttcatgt
     3241 ccgaagccct cctcatcgct ctcatcaagg gcattgccgt cgggatcgtc atcgcgctcc
     3301 cggccgggcc ggtcggcgtg ctgtgtgtcc ggcgcacgct cttcgaaggt ccggtctacg
     3361 gcttcgtctc cggcctgggc gcggccaccg cggacacgct cttcgccgtc gtcgccggtt
     3421 tcggcgtcac catcgtgcgc gacttcatgc tgcgctttca ggactggttc ggcgcg
//
LOCUS       DASYNS010000498         3742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2309288, whole genome shotgun sequence.
ACCESSION   DASYNS010000498 DASYNS010000000
VERSION     DASYNS010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309288"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VGP50_15460"
     CDS             complement(<1..500)
                     /locus_tag="VGP50_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019088621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEV8028830.1"
                     /translation="MFDKLLIANRGEIAVRIIRAARELGLKTVAVFSEADRESLAVRL
                     ADEAVAIGPAPASKSYLNVAAIIAAARQSGAGAIHPGYGFLAENARFAEAVEGAGLAF
                     VGPAPKTIRMMGDKAAARAAAQRAGVPIVPGSAGEVQGLDAAREAARDIGYPVMIKAS
                     AGGGGRG"
     gene            complement(512..757)
                     /locus_tag="VGP50_15465"
     CDS             complement(512..757)
                     /locus_tag="VGP50_15465"
                     /inference="COORDINATES: protein motif:HMM:NF005457.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEV8028831.1"
                     /translation="MAQHQVVSPLPGIFYRRPSPEAAPYKKEGDRVKEGDVIGLVEVM
                     KQFTEIQADADGTVVRFLTDNEAPVEPEQPLLLIETD"
     gene            complement(791..1537)
                     /gene="pxpA"
                     /locus_tag="VGP50_15470"
     CDS             complement(791..1537)
                     /gene="pxpA"
                     /locus_tag="VGP50_15470"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEV8028832.1"
                     /translation="MPAKGNAMPLAINCDMGESFGLYKMGDDEGMMPYITIANVACGF
                     HASDPTVMARTVRLAKRHKVNVGAHPSLPDLQGFGRREMKMQPEELTDCIVYQVGALK
                     GFLDREGMPLFHVKPHGSLYGMAARSPEVAKAVAEAAKVFNVPVLGMAGTVHETVYTA
                     EGLSFISEYYADLDYADDGTLIITREHHAVDPTRAAERARRAVREGKVESINGKEVRV
                     RADCICVHSDTPNAVEVARAVKTAVAEYLA"
     gene            complement(1549..2565)
                     /locus_tag="VGP50_15475"
     CDS             complement(1549..2565)
                     /locus_tag="VGP50_15475"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8028833.1"
                     /translation="MTVATHKAAGTATWWQQAVVRLGVLPLLLAIAVIVFSLLSPNFL
                     TGQNLLNVARQSTFLAMVAMGQMLALLTGGFDLSVGKTLAITSVVGALVMAGAKSSFS
                     DAVGVAIALGMLAGVGAGTLVGIVNGIGVALFDVSPFMMTLGTASISFGIALTLTSGV
                     PVYGMPDAFGNIFGFGQFLHIPSPVYVTAILFLALYLVLNWTPMGRYFYAIGGNHKAA
                     VLSGINTRFHLFMAYVLCGLMAAIAGIILTARLETGEANIGASMPLDSIAACVIGGVS
                     LRGGVGRLGNVVLGAVFIGLIQNGMDLARIASYLQEVVIGVLLILAVIADQLRHRLLL
                     RLKD"
     gene            complement(2562..>3742)
                     /locus_tag="VGP50_15480"
     CDS             complement(2562..>3742)
                     /locus_tag="VGP50_15480"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEV8028834.1"
                     /translation="HGLLNKRALRRRAQEILDRLGFALRPGERVQYLSRAQQQMVEIA
                     KAFRAEPSVLILDEPTASLAEQEAERLFALVEQIKRRGVGIIYVSHRMNENRRIGDRI
                     TVLRDGRRIETLAMDEADDERLLQLMTGRVITQIFPRIDHSPGRVLLEVDGISTADET
                     VRDVSLGVRAGEIVGLAGLVGCGKSEVGRACFGLETLAAGAIRFDDADVTRATPKQMI
                     ERGLFYVPPDRREEGLVMVRSVRENVALPALDRPPLSRFGILDRAAERRTVRPLAERL
                     NLQPRNIERDVQYFSGGNQQKVMLAKGLTRPIRLFIFDEPTVGVDVGTRIAIYEFIRD
                     LCQAGAGILLISSELPEILHLTNRTYVMYRGQVQGELGGAQMTQDAVLGHFFERRAGR
                     "
BASE COUNT          611 a         1286 c         1263 g          582 t
ORIGIN      
        1 ccgcgcccgc cgccgccggc gctcgccttg atcatcacgg gatagccgat gtcgcgggca
       61 gcttcgcgcg ccgcatcgag gccctggacc tccccggcgc tgccggggac gatcggcacc
      121 cctgcccgct gcgccgccgc gcgcgccgcc gccttgtcgc ccatcatgcg gatcgtcttg
      181 ggcgcggggc cgacgaaggc gaggccggca ccttccaccg cctccgcgaa acgcgcattc
      241 tccgcaagaa agccgtagcc gggatggatc gcgccggcgc cgctctgccg cgccgcggcg
      301 atgatcgccg cgacgttgag atagctcttg ctcgccggcg ccgggccgat ggcgaccgcc
      361 tcgtcggcga gccgcaccgc gaggctttcc cggtcggcct cgctgaacac cgcgaccgtc
      421 ttgaggccaa gctcgcgcgc cgcccggatg atccggacgg cgatctcgcc gcgattggcg
      481 atcaggagct tgtcgaacat gccgcgcggt ctcagtcggt ctcgatcagc agcagcggct
      541 gctcgggctc gaccggcgcc tcgttgtcgg tgaggaagcg cacgaccgtg ccgtcggcat
      601 cggcctggat ctcggtgaac tgcttcatca cttcgacgag gccgatgaca tcgccctcct
      661 tcacccggtc gccctctttc ttgtaaggcg ccgcctcggg cgaggggcgc cgatagaaga
      721 ttccgggcaa cggactgacc acctgatgct gcgccatccc tcgattccct ttctcctgtg
      781 gtggccgaaa ctacgccaga tactcggcga ccgccgtctt gaccgcgcgc gcgacctcga
      841 ccgcgttggg tgtgtcggaa tggacgcaga tgcagtcggc gcggacgcgg acctccttgc
      901 cgttgatgga ctccaccttt ccctcccgga ccgcgcgccg ggcgcgctcc gccgcccgcg
      961 tcggatcgac ggcgtggtgc tcgcgggtga tgatcagggt gccgtcatcg gcatagtcga
     1021 gatcggcgta gtattcggaa atgaagctca ggccttcggc ggtgtagacc gtctcgtgca
     1081 cggtgccggc catgcccagc accggcacgt tgaacacctt cgcggcttcg gccaccgcct
     1141 tcgccacttc gggcgagcgc gccgccatgc cgtagagcga gccgtgcggc ttgacgtgaa
     1201 agagcggcat tccctcgcgg tcgagaaagc ccttgagcgc gcccacctgg tagacaatgc
     1261 agtcggtgag ctcctcgggc tgcatcttca tctcgcgccg gccgaaaccc tggaggtccg
     1321 gcagcgaggg atgggcgccg acattgacct tgtgccgctt ggcgagcctg accgtgcgcg
     1381 ccatcaccgt cggatccgac gcatggaagc cgcaggcgac attggcgatg gtgatgtagg
     1441 gcatcatgcc ctcgtcatcg cccatcttgt agaggccgaa gctctcgccc atgtcgcaat
     1501 tgatggcgag cggcatcgcg tttcccttgg ccggcattgg cgcctccttc aatccttgag
     1561 cctgagcagc aagcgatggc gcagctggtc ggcgatgacc gccaggatga gcaggacccc
     1621 gatcacgacc tcctgcaggt aggacgcgat ccgcgccagg tccatgccgt tctggatgag
     1681 gccgatgaac accgcgccca ggacgacgtt gccgaggcgg ccgacgccgc cgcgcaggct
     1741 gacaccgccg atgacgcagg cggcgatcga atcgagcggc atcgacgcgc cgatattggc
     1801 ctccccggtc tcgagccgcg ccgtgaggat gattccggcg atcgccgcca tcaagccgca
     1861 cagcacatag gccatgaaga gatgaaagcg agtgttgatg ccggacagca ccgcggcctt
     1921 gtggttgccg ccgatggcat agaaatagcg tcccatcggc gtccagttga gaacgaggta
     1981 gagcgccagg aagaggatcg cggtgacgta gaccggcgac ggtatgtgga ggaattgccc
     2041 gaagccgaag atgtttccga acgcatcggg catgccatag accgggacgc cgctggtcag
     2101 ggtcagcgcg atgccgaagc tgatggaggc agtgccgagg gtcatcatga agggcgagac
     2161 gtcgaagagc gcgacgccga tgccgttgac gatgccgacc agcgtgccgg cgccgacgcc
     2221 cgcgagcatg cccagcgcga tcgccacgcc gacggcatcg gaaaagctcg atttcgcccc
     2281 ggccatcacc agggcgccga ccaccgaggt gatggccagc gtcttgccga ccgacagatc
     2341 gaagccgccg gtgagcagcg ccagcatctg gcccatcgcc accatggcga gaaaggtcga
     2401 ctggcgggcg acgttgagca ggttctgccc ggtgaggaag ttcggcgaca gcagactgaa
     2461 gacgatgacc gcgattgcca gcagcagcgg caggacaccg agcctgacga cggcctgctg
     2521 ccaccaggtg gcggtccccg ccgccttatg cgtcgcgacc gtcatcgccc cgcccgccgc
     2581 tcgaagaaat gaccgagcac cgcgtcctgc gtcatctgcg cgccgccgag ctccccctgc
     2641 acctggccgc gatacatgac ataagtgcgg ttggtcaggt gcaggatctc gggcagctcc
     2701 gaggagatga gcaggatgcc ggcgccggcc tggcagagat cgcggatgaa ttcgtagatg
     2761 gcgatgcgcg tgccgacatc gacgccgacc gtcggctcgt cgaagatgaa gagccggatc
     2821 ggccgcgtca ggcccttggc cagcatcacc ttctgctgat tgccgcccga gaagtactgc
     2881 acgtcgcgct cgatgttgcg cggctggagg ttgagccgct cggcgagcgg ccgcacggtg
     2941 cggcgctcgg cggcacggtc gagaatgccg aagcgcgaca gcggcggccg gtcgagcgcc
     3001 ggcagtgcca cgttctcgcg cacgctgcgc accatgacca acccttcctc gcgccggtcg
     3061 ggcggcacat agaagaggcc gcgctcgatc atctgctttg gcgtggccct cgtgacatcg
     3121 gcatcgtcga agcggatggc accggcggcc aatgtctcga ggccgaagca cgcccgcccg
     3181 acctcggact tgccgcagcc gaccaacccg gcaagcccga cgatctcgcc ggcccgcacg
     3241 ccgagcgaca cgtcccggac cgtctcgtcc gcggtgctga tgccgtcgac ctcgagcaga
     3301 acgcggccgg gtgaatggtc gatgcgcgga aagatctggg tgatgacccg ccccgtcatg
     3361 agctggagca gccgctcgtc gtcggcctcg tccatcgcca gcgtctcgat gcgccggccg
     3421 tcgcgcagca cggtgatgcg gtcgccgatg cggcggttct cgttcatgcg gtggctgaca
     3481 tagatgatgc ccacgccccg ccgcttgatc tgctcgacga gcgcgaagag ccgctccgcc
     3541 tcctgctcgg ccagcgatgc cgtcggctcg tcgaggatga gcaccgacgg ctcggcgcgg
     3601 aaggccttgg cgatctccac catctgctgc tgggcgcgcg acaggtactg gacgcgctcg
     3661 cccggccgca gcgcgaagcc gagccggtcg aggatctcct gggcgcgccg gcgcagcgcg
     3721 cgcttgttga gcagcccatg cg
//
LOCUS       DASYNS010000499         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_435853, whole genome shotgun sequence.
ACCESSION   DASYNS010000499 DASYNS010000000
VERSION     DASYNS010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435853"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /gene="ureC"
                     /locus_tag="VGP50_15485"
     CDS             complement(<1..1655)
                     /gene="ureC"
                     /locus_tag="VGP50_15485"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007003034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEV8028835.1"
                     /translation="MTTRISRAAYADMFGPTTGDRVRLADTELFIEVEEDRTVYGEEV
                     KFGGGKVIRDGMGQSQRSRAEGAVDTVITNALILDHWGIVKADIAIKDGRIAAIGKAG
                     NPDVQPGVDIVIGPGTEAIAGEGKIVTAGGIDVHMHMICPQQIEEALYSGVTTMMGGG
                     TGPASGTAATTCTPGPWHIARMLQAIEAFPMNIGLFAKGNATLPQALVEQIEAGACAM
                     KLHEDWGTTPAAIDNCLSVADRYDVQVAIHTDTLNESGFVEDTIAAFKGRTIHAFHTE
                     GAGGGHAPDIIRVCGEANVLPSSTNPTRPYTVNTLDEHLDMLMVCHHLDGRIPEDVAF
                     AESRIRKETIAAEDILHDLGAFSMISSDSQAMGRVGEVIIRCWQTADKMKRQRGRLSG
                     EIGDNDNLRAKRYVAKYTINPAIAQGISKHVGSIETGKLADLVVWSPAFFGVKPDLVL
                     KLGSIAAAPMGDPNASIPTPQPVHYRPMFAGFGRHVQASAVTFVSGAAAASDVAARLG
                     LSKPLVAVENTRGGISKKSMVLNDRTPRIEVDAETYEVRADGDL"
     gene            complement(1652..1960)
                     /locus_tag="VGP50_15490"
     CDS             complement(1652..1960)
                     /locus_tag="VGP50_15490"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEV8028836.1"
                     /translation="MIPGEIIAKSGEIELNAGRAAVEIEVANTGDRPIQVGSHYHFFE
                     TNAALRFDRAKARGCRLDIPAGTAVRFEPGQTRKVRLVGYAGARIVHGFNKKIEGPLA
                     "
     gene            complement(1976..2278)
                     /locus_tag="VGP50_15495"
     CDS             complement(1976..2278)
                     /locus_tag="VGP50_15495"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEV8028837.1"
                     /translation="MNLTPREKDKLLVAMAAIVARRRLERGVKLNHPEAVALITDYVV
                     EGARDGRSVADLMRDGATVLKREQVMEGIAEMIHDIQVEATFPDGTKLVTVHRPIR"
     gene            complement(2291..>2539)
                     /locus_tag="VGP50_15500"
     CDS             complement(2291..>2539)
                     /locus_tag="VGP50_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEV8028838.1"
                     /translation="GAAALATAVYVGADAAAHLALARALAEGGDCRGGATLLGPVLVA
                     RFLDARAEIVRAALARYVAGLRHAAAGLPAVLPRLWRI"
BASE COUNT          377 a          843 c          875 g          444 t
ORIGIN      
        1 aggtcgccgt cggcacgcac ttcgtaggtc tcggcatcga cctcgatgcg cggcgtccgg
       61 tcgttcagga ccatgctctt cttcgagatg ccgccgcgcg tgttctcgac ggcgacgagc
      121 ggcttcgaca ggccgagccg cgccgccacg tccgacgcgg cggcggcgcc gctcacgaag
      181 gtgacggcgc ttgcctgcac atgccggccg aagccggcga acatgggacg gtaatgcacc
      241 ggctgcggcg tcgggatcga ggcgttggga tcgcccatgg gcgccgcggc gatgctgccg
      301 agcttgagca ccaggtcggg cttgacgccg aagaaggccg gcgaccagac cacgagatcg
      361 gcgagcttgc ccgtctcgat cgagcccaca tgcttgctga tgccctgcgc gatcgccgga
      421 ttgatcgtgt atttcgcgac atagcgcttg gcgcggagat tgtcgttgtc gccgatctcg
      481 cccgacagac ggccgcgctg gcgcttcatc ttgtcggcgg tctgccagca gcggatgatg
      541 acttcgccga cgcggcccat cgcctggctg tcggaagaga tcatcgagaa ggcgccgaga
      601 tcgtgcaaaa tgtcctcggc ggcgatcgtc tccttgcgga tgcggctttc ggcgaaagcc
      661 acgtcctcgg ggatcctgcc gtcgagatga tggcatacca tcagcatgtc gagatgctcg
      721 tcgagcgtgt tgacggtgta gggccgcgtc ggattggtcg aagaaggcag cacattcgct
      781 tcgccgcaga cgcgaatgat gtcgggcgcg tggccgccgc cggcgccctc ggtgtggaag
      841 gcatgaatgg tgcggccctt gaaagcggcg atcgtatcct cgacgaaacc cgattcgttg
      901 agcgtgtcgg tatggatcgc gacctgcacg tcgtagcggt cggcgacgct caggcaattg
      961 tcgatggcag ccggcgtcgt gccccaatcc tcgtgcagct tcatggcgca ggcgcccgcc
     1021 tcgatctgct cgacgagcgc ctgcggcaag gtggcgttgc ccttggcgaa gaggccgata
     1081 ttcatcggga acgcctcgat cgcctgcagc atccgggcaa tatgccaggg ccccggcgtg
     1141 caggtggtcg cggccgtgcc ggaagcaggg ccggtgccgc cgcccatcat cgtcgtgacg
     1201 cccgagtaaa gcgcctcttc gatctgctgc gggcagatca tgtgcatatg cacgtcgatg
     1261 ccgccggcgg tgacgatctt gccttcgccg gcgatcgcct cggtgcccgg gccgatgacg
     1321 atgtcgacgc ccggctgcac atcgggattg ccggccttgc cgatggcggc gatccggccg
     1381 tccttgatgg cgatatcggc tttgacgatg ccccaatgat cgaggatcag cgcgttggtg
     1441 atgacggtgt cgacggcgcc ctctgcacgg cttcgctggc tctggcccat gccgtcgcgg
     1501 atcaccttgc cgccgccgaa cttgacctcc tcgccataga cggtgcggtc ctcctcgacc
     1561 tcgatgaaga gctcggtatc ggcgagacgc acgcggtcac cggtggtggg gccgaacata
     1621 tcggcatagg cggcgcggct gatgcgggtc gtcatgcgag cggaccttcg atcttcttgt
     1681 tgaagccatg cacgatgcgc gccccggcat agccgacgag ccgcaccttg cgcgtctgcc
     1741 cgggctcgaa gcgcacggcc gtaccggcgg ggatgtcgag gcggcagccg cgcgccttcg
     1801 cgcggtcgaa gcgcagcgcc gcattggtct cgaagaaatg gtagtgcgag ccgacctgga
     1861 tcggccggtc gcctgtgttg gcgacctcga tctccactgc ggcccggccg gcgttgagct
     1921 cgatctcgcc gctcttcgcg atgatttcgc cggggatcat ttcatcatct ccctgtcatc
     1981 tgatcggccg atgcacggtg acgagcttgg tgccgtcggg gaaggtggct tcgacctgga
     2041 tatcgtggat catctcggcg atgccctcca tcacctgctc gcgcttgagc accgtcgcgc
     2101 cgtcgcgcat caggtcggcg acggagcggc cgtcccgcgc gccttcgacg acgtagtccg
     2161 tgatgagcgc cacggcctcg gggtgattga gcttgacgcc gcgctcgagc cgccggcgcg
     2221 ccacgatcgc ggccatggcg acgagcagct tgtccttttc ccgcggcgtc agattcatcg
     2281 ctctccctcc tcagatcctc cagagccgcg gcagcactgc cggcaatccg gccgccgcat
     2341 ggcggaggcc cgcgacatag cgggcgagcg cggcacggac aatctcggcg cgcgcatcga
     2401 ggaatcgcgc gacgagcacg gggccgagca gcgtcgcgcc gccgcgacag tcgccgccct
     2461 cggcgagcgc gcgggccagc gccagatgcg cggccgcgtc ggcgccgaca tagaccgcgg
     2521 tcgccagcgc cgcggcgcc
//
LOCUS       DASYNS010000500         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_593580, whole genome shotgun sequence.
ACCESSION   DASYNS010000500 DASYNS010000000
VERSION     DASYNS010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593580"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..968
                     /locus_tag="VGP50_15505"
     CDS             261..968
                     /locus_tag="VGP50_15505"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEV8028839.1"
                     /translation="MPNTVSAVSRQLKLLRQRAGLSIREVAQALGMEHGSSYQHYEDR
                     FKKPLLPLDLVMKLVPIFEAGGVEASELYALAGVSATGERPLVPASPRGDAGGRMVRI
                     AELDVRASAGGGLLGENEKMIAEWQVPSGMVRSHSTAPAADLRIITVMGDSMEPTLQP
                     GQRVLIDTGDRKPSPPGIFVVWDGLGLVIKRVQMVPHSEPPKVKITSDNAKYEGYERS
                     LDEAYIQGRVIGQWRWL"
     gene            1110..1427
                     /locus_tag="VGP50_15510"
     CDS             1110..1427
                     /locus_tag="VGP50_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028840.1"
                     /translation="MKRVLLSAVLSIGCLSAAIAGENQAYQCQRSDDSRTVAIEYSTT
                     DGAQSCSVMYKKFANSSEPAKELWHYQAHADQCDVQAKQFMTKLEGFGLSCSPTQATA
                     MKQ"
     gene            complement(1523..2287)
                     /locus_tag="VGP50_15515"
     CDS             complement(1523..2287)
                     /locus_tag="VGP50_15515"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV8028841.1"
                     /translation="MAGRLAGKAAFITGGASGLGRAMATAFAAEGARVAIADIDAARG
                     REAARSIGERAIFLAHDVTDEEQWIANLAEAARVFGRLDTLVNNAGIGTRGSVETTTL
                     AEWRRIHAVNLDGPFLGCKHAIPFIAAAGGGAVVNISSVAGLIGARDSAAYCASKGGL
                     RLLTKSVALHCAHRRNGVRCNSIHPVYTETPMVEEMLAASRDPQKMREAMAAMIPLGR
                     LGTPEELAAMAVYLVSDEARFVTGAEFVFDGGYTAN"
     gene            complement(2302..>2491)
                     /locus_tag="VGP50_15520"
     CDS             complement(2302..>2491)
                     /locus_tag="VGP50_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568426.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV8028842.1"
                     /translation="KPGQQPSKESILAFLGDKIAKWQLPDDVVFLPEIPHGATGKVLK
                     TRLRDQFKDYRLPPGQGA"
BASE COUNT          423 a          849 c          806 g          413 t
ORIGIN      
        1 ccgtcccgcc ctttccccct agcgaccccg ccttcctccg ccctcctccc ggccgccgcc
       61 cggtctcacc ggcatgcggc gtcagaccgc gtgacaagcc ccccgcagcg cgctatcgtt
      121 ctcgttgtgt tctaacccaa tgctatacgg tcaagggtgc ggcatgccga cgcgcggtcg
      181 aacgatatac gcacaaaacg tgcaacgctc cattcaaaaa gcgtctggaa acgtattggt
      241 tcggcgtaca gactgcggcc atgcccaata ccgtttccgc ggtttcccgc cagttgaagc
      301 tgttgcgcca acgcgccggc ttgtcgatcc gggaggtggc gcaggcgctc ggcatggagc
      361 atggcagcag ctaccagcat tatgaggacc gcttcaaaaa accgctgctg ccgctcgacc
      421 tcgtcatgaa gctggtgccg atcttcgagg cgggcggcgt cgaggcgagc gagctctatg
      481 cgcttgccgg cgtcagcgcc acgggcgagc gcccgctcgt tccggcgtcg ccgcgcggcg
      541 acgcgggcgg ccgcatggtg cgcatcgccg agctcgacgt gcgcgccagc gccggcggcg
      601 gcctcctcgg cgagaacgag aagatgattg ccgaatggca ggtgccgagc ggcatggtgc
      661 gcagccacag cacggctcca gcggccgatc tccgcatcat cacggtgatg ggcgacagca
      721 tggagccgac gctgcagccg ggccagcgcg tcctcatcga caccggcgac cgcaagccca
      781 gcccgcccgg cattttcgtg gtgtgggacg ggctcggcct cgtcatcaag cgcgtgcaga
      841 tggtgccgca cagcgagccg cccaaggtca agatcacctc cgacaacgcc aaatacgagg
      901 gctatgagcg ctcgctcgac gaggcctata tccaaggtcg cgtcatcggg cagtggcgct
      961 ggctctgatt cccggcgata cccgggattt cgcccgaaga cgcggaaaac cccggcgttt
     1021 tcgggttttg atacgccgcc attaagcacc gggtaaggag gggctcctag catcgcgcca
     1081 ccgaacgcga tttcaatgga ggttggctga tgaagcgtgt ccttctgtct gccgtcctga
     1141 gcatcggctg cctgagcgcg gccatagcgg gcgaaaacca ggcttaccag tgccagcgca
     1201 gtgacgactc gcgcaccgtc gccatcgagt actcgacgac ggacggcgca cagagctgca
     1261 gcgtcatgta caagaaattc gccaactcgt ccgagccggc gaaggagctc tggcattacc
     1321 aggcccatgc cgaccaatgc gacgtccagg ccaagcagtt catgaccaag ctcgagggct
     1381 tcggcttgtc ctgctccccg acccaagcga cggcgatgaa gcagtagtct ccgcctctgc
     1441 ccccttgacg ctcgtcccgg agcctggctc cgggacgaac gcaaggaaca tgcgatttta
     1501 cgctatcccc gtttgatccc gactaattcg ccgtatagcc gccgtcgaac acgaattcgg
     1561 cgccggtgac gaaccgcgcc tcgtcggaaa cgagataaac ggccatggcc gccagctctt
     1621 cgggcgtgcc gagccggccg agcggaatca tcgccgccat cgcctcgcgc atcttctgag
     1681 gatcgcgcga cgcggccagc atctcctcga ccatcggcgt ctcggtgtag acggggtgga
     1741 tcgagttgca gcgcacgccg ttgcggcgat gcgcgcaatg cagcgccacc gatttggtga
     1801 ggaggcgcaa gccgcccttg ctcgcgcaat aggcggcgct gtcgcgcgca ccgatgagcc
     1861 ccgcgacaga ggagatgttg acgacggcgc cgccgcccgc ggcggcaatg aacgggatcg
     1921 cgtgcttgca gccgagaaaa gggccgtcga gattgacggc gtggatgcgg cgccattcgg
     1981 cgagggtcgt cgtctcgacg gagccgcgcg tgccaatgcc ggcgttgttg acgagcgtgt
     2041 cgagacggcc gaagacgcgc gccgcctcgg cgagattggc gatccactgc tcctcgtcgg
     2101 tcacgtcgtg agcgaggaag atcgcacgct cgccgatgct ccgcgccgcc tcgcgtcccc
     2161 gcgcggcgtc gatatcggcg atggcgacgc gggctccctc tgccgcaaag gccgtcgcca
     2221 tggcccgccc caggcccgaa gcgccgccgg tgatgaacgc cgccttgccg gcaagccgtc
     2281 ccgccatgct ctgctccgtt ctcaggcgcc ttgcccgggc ggcagccggt aatccttgaa
     2341 ttggtcgcgc agccgcgtct tcaggacctt gcccgtggcg ccgtggggta tctccggcag
     2401 gaagacgacg tcgtcgggca gctgccattt ggcgatcttg tcgccgagga aggcgaggat
     2461 gctctctttg ctcggctgct gccccggctt g
//
LOCUS       DASYNS010000501         4116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_283549, whole genome shotgun sequence.
ACCESSION   DASYNS010000501 DASYNS010000000
VERSION     DASYNS010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283549"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..523)
                     /locus_tag="VGP50_15525"
     CDS             complement(107..523)
                     /locus_tag="VGP50_15525"
                     /inference="COORDINATES: protein motif:HMM:NF022227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliX"
                     /protein_id="HEV8028843.1"
                     /translation="MRIEGPPPVRRTAVRRDGDKAEAAGDKFAAALDDSEPAAAAAQS
                     PAVSALEGLLSIQEMPDAFVGRRRAMLRADVLLDRLEDLRLGLLAGVVPRDRLEELAR
                     LARTARDTVDDPRLAAILDQIDLRAAVELAKLDTGR"
     gene            740..1936
                     /locus_tag="VGP50_15530"
     CDS             740..1936
                     /locus_tag="VGP50_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HEV8028844.1"
                     /translation="MFHRLAPLHRLGLALAATGLLLGAATPALASSRIKDIADFEGVR
                     DNMLVGYGLVVGLNGTGDSLNNAIFTRESLIGMLERLGVNARDKSLRTANVAAVMVTA
                     TLRPFARQGGRLDVAVSALGDCKSLQGGMLLVTPMLGADGEVYAVAQGSVASGGFAAK
                     GQGGSVTKGVPTSGRVSAGAIVEREVGFELTRLPTINLMLRNPDFTTARRVAQAVNGY
                     LKSPIAHALDSSTVLITIPIELKADLVGFLTDIEQLRVEPDQVARVVIDEANGVIVMG
                     ENVRISTVAVAQASLTVKITETPQVSQPNPFSRPGPAGAVTPRAKGGVQFAPGGGAQT
                     VVTPRTEIKVDEQGEHRVAVLPVGVTLQELVNSLNALGVGPRDMISILQAIKTAGALQ
                     AEIELM"
     gene            1936..2253
                     /locus_tag="VGP50_15535"
     CDS             1936..2253
                     /locus_tag="VGP50_15535"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HEV8028845.1"
                     /translation="MDAGTPLLSPLTGPAQTAAPPATIATSDASAAHKAATDFEAFFL
                     SQTFESMFSGVNTDSMFGGGNGETVYRSLLLQEYSKVAAKNGGVGIADAVQREILRAQ
                     EGK"
     gene            2250..2654
                     /locus_tag="VGP50_15540"
     CDS             2250..2654
                     /locus_tag="VGP50_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028846.1"
                     /translation="MSGAALPEVMDQLAELLAAETQLVTAGRVTEIAPMQSEKLRLAR
                     LYEQAFRAFDPNNAPASARTPALSVAAARLARAATENERALRIGGAATRHLIEMLVDS
                     IKSQQGAVRGYGATRAPSRPAPMRPVAVNRRA"
     gene            2658..>4116
                     /gene="flgK"
                     /locus_tag="VGP50_15545"
     CDS             2658..>4116
                     /gene="flgK"
                     /locus_tag="VGP50_15545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HEV8028847.1"
                     /translation="MSLGVALANAVSGLTAAQANLTLISGNIANAQTPGYSRETLPQT
                     SQVLDGNGSGVDTGVARRVSDQVLTASVRSQTSVTAAANTLNTYFQQIQNLFGQVGDA
                     NSLPDTLNQFSSALQTLATTPEDTVAQSNAVDAGQALASQLNGMSSAVQTLRSNADAE
                     IGTSVTDANQLLTNIAGYNGAIARAQTTGQSTATIADQLDQALAQLAQQMNITSFSRP
                     DGSVVVMTGGGKTLVDGVTAQQLSFTPSSSVAAGSVVSGITVNGIAVTGDITSGNIAG
                     LLQMRDTQLPALTAELNQFTNQLFNTTKVTQSTQLVTVGGGAPNAGDTFQVTIDGQVF
                     NTAGIAGNTVAGIVNAINTQLGATTFSASVSNGGILITDSAGNPLTATIVQTGGTGTA
                     TFTPQSPANALPTTNSGTSGAAPGDANAFFAGVNTATGLDNAGTIAVNPSLAANPALL
                     DGVANVPSPTIAAALADALSLNTPTFAAAGNIPAAQ"
BASE COUNT          695 a         1532 c         1288 g          601 t
ORIGIN      
        1 aaagcgcgaa cctgcgaagg cgtgcgagta tatgaatcgt gccctgcttg agcaagcgcc
       61 tcaaatcatt acacttcttt gaaatcactc aatattcccc gctgcctcag cggccggtgt
      121 cgagcttcgc cagctcgaca gccgcgcgca ggtcgatctg gtccagaatg gcggcgaggc
      181 gcggatcgtc gacggtgtcg cgggccgtcc gggcgagccg ggccagctcc tcgagccggt
      241 cgcgcggcac aaccccggcg agcaggccca ggcgcagatc ctcgagccgg tccagcagga
      301 catcggcccg cagcatggcg cggcgccgcc cgacgaaggc gtccggcatc tcctggatgc
      361 tgagaagccc ttcgagcgca ctgaccgccg ggctttgcgc cgcggcagcc gccggctcgc
      421 tgtcgtcgag cgccgctgcg aatttgtcgc cggccgcttc ggccttgtcg ccgtcgcggc
      481 gcacggccgt ccgccgcacg ggcgggggtc cctcgattct catcgctggc ggaagccttt
      541 cgctgtcatg gagcgaagca tcgtcaatcg ctggttaagg gtggcttaag gcgggcaaaa
      601 cttgccggcc cgcggcaggt tttgccggtc gcgcggcctt cgctccggct cggaaaccat
      661 tgaaaaatca gcaaatttcc cgtggcacga ctctggcaag agcgagttgc cagaactcac
      721 tatcgccagg ccgcccctta tgttccatcg cctcgccccg ttgcaccgtc tcggcctcgc
      781 gcttgccgcc accggcctgc tgctgggcgc tgccacgccg gcgcttgcct cctcgcgcat
      841 caaggacatc gccgatttcg agggcgtgcg cgacaacatg ctggtgggct atggcctcgt
      901 ggtcggcctg aacggcaccg gcgacagcct caacaacgcg atcttcaccc gagagagcct
      961 catcggcatg ctcgagcgcc tcggcgtcaa tgcgcgcgac aagagtctgc gcaccgccaa
     1021 tgtcgccgcg gtcatggtga cggcgacgct gcggcccttc gcccgccaag gcggccgcct
     1081 cgacgtcgcg gtctcggcgc tcggcgattg caagagcctg caaggcggca tgctgctggt
     1141 gacgccgatg ctcggcgccg atggcgaggt ctatgccgtg gcgcagggct ccgtcgcgag
     1201 cggcggcttc gccgccaagg gccagggcgg ttccgtcacc aagggcgtgc cgacctcggg
     1261 ccgcgtctcg gccggcgcca tcgtcgagcg cgaggtcggt ttcgagctca ccaggctccc
     1321 caccatcaac ctgatgctgc gcaaccccga tttcacgacg gcgcgccgcg tcgcgcaggc
     1381 cgtcaacggc tatctcaaga gcccgatcgc gcacgcgctc gactcctcga ccgtgctcat
     1441 caccatcccg atcgagctca aggccgacct cgtcggcttc ctcaccgaca tcgagcagct
     1501 ccgcgtcgag cccgaccagg tggcgcgcgt cgtcatcgac gaagcgaatg gcgtcatcgt
     1561 catgggcgag aatgtccgca tcagcaccgt ggccgtggcg caagcgagcc tcaccgtcaa
     1621 gatcaccgag acgccgcaag tgtctcagcc caatcccttt tcgcggccgg gcccggcggg
     1681 cgcagtgacg ccgcgggcca aaggcggcgt gcaattcgcc cccggcggcg gcgcgcagac
     1741 cgtcgtcacg ccgcgcaccg agatcaaggt cgacgagcag ggcgagcacc gcgtcgccgt
     1801 gctgccggtc ggcgtcaccc tccaggagct cgtgaacagc ctcaacgctc tgggcgtcgg
     1861 ccctcgcgac atgatcagca tccttcaggc gatcaagacc gcgggcgcgc tccaggccga
     1921 gatcgagctg atgtgatgga cgccggcacc ccccttctgt cgcccctgac cggtccggcg
     1981 cagacggccg cgccgccggc gacgatcgcg acgagcgatg cgtccgccgc gcacaaggcg
     2041 gcgacggatt tcgaggcgtt ctttctttct caaaccttcg agagcatgtt cagcggcgtc
     2101 aacaccgact cgatgttcgg cggcggcaat ggcgagaccg tctaccgctc gctgctgctt
     2161 caggaataca gcaaggtcgc ggcgaagaat ggcggcgtcg gcatcgccga cgcggtacag
     2221 cgcgagatcc tgcgcgctca ggagggaaaa tgagcggcgc cgctttaccc gaggtcatgg
     2281 accagctcgc cgagctgctc gcggccgaga cccagctcgt gacggcaggc cgcgtcaccg
     2341 agatcgcgcc gatgcagagc gagaagctgc gcctcgcgcg gctctacgag caggccttta
     2401 gggccttcga ccccaacaat gccccggctt cggcgcgcac gcctgcattg tccgtcgcgg
     2461 ccgcgcggct cgcgcgggcc gccaccgaga acgagcgcgc cttgcgcatc ggcggcgcag
     2521 cgacgcggca cctcatcgag atgctcgtcg attcgatcaa atcgcagcag ggcgccgtgc
     2581 gcggctatgg cgcgacgcgc gcaccgtcgc ggccggcgcc gatgcggccg gtcgccgtca
     2641 accggagggc ctgagccatg agtctcggag tcgcccttgc caatgccgta agcggcctca
     2701 ccgccgcgca ggccaatctg acgctgatct ccggcaatat cgccaacgcc cagacccccg
     2761 gctattcgcg cgagacgctg ccgcagacgt cgcaggtcct cgacggcaac ggcagcggcg
     2821 tcgacaccgg cgtggcgcgg cgcgtcagcg atcaggtcct gaccgcgagc gtgcgctccc
     2881 agaccagcgt caccgccgcc gccaacacgc tcaacaccta tttccagcaa atccagaacc
     2941 tcttcggcca ggtcggcgac gccaattcgc tgcccgacac gctcaaccag ttctcgagcg
     3001 cgctgcagac gctcgccacc acgcccgaag acacggtcgc gcagagcaac gccgtcgatg
     3061 ccggccaggc cctcgccagc cagctcaacg gcatgtcctc ggcggtccag actctgcgca
     3121 gcaatgccga tgccgagatc ggcacgtcgg tgaccgatgc gaaccagctc ctcaccaaca
     3181 tcgccggcta caacggcgcg atcgcccgtg cccagacgac agggcaatcg acggcgacca
     3241 tcgccgatca gctcgaccag gcgctggcgc agcttgcgca gcagatgaac atcaccagct
     3301 tcagccgccc cgatggcagc gtcgtcgtca tgaccggcgg cggcaagacg ctggtcgacg
     3361 gcgtcaccgc ccagcagctc agcttcacgc cctcgagctc cgtcgccgcc ggcagcgtcg
     3421 tctcgggaat caccgtcaac ggcatcgccg tcaccggcga catcacgagc ggcaacatcg
     3481 ccggcctctt gcagatgcgc gacacgcagc tccccgcgct cactgccgag ctcaaccagt
     3541 tcacgaacca gctcttcaac accacgaagg tgacgcagtc gacgcagctc gtgaccgtcg
     3601 gcggcggcgc tcccaacgcg ggcgacacct tccaggtgac gatcgacggc caggtcttca
     3661 acaccgccgg catcgccggc aacaccgtcg ccggcatcgt caacgccatc aacacgcagc
     3721 tcggcgccac gaccttctcc gccagcgtca gcaacggcgg catcctcatc acggattccg
     3781 ccggcaatcc gctcacggcg accatcgttc aaacgggcgg cacgggcact gcgaccttca
     3841 cgccgcaatc gcccgcgaat gcgctcccca ccaccaacag cggcacgagc ggcgccgcgc
     3901 cgggcgatgc caatgccttc ttcgccgggg tcaacacggc gaccgggctc gacaatgccg
     3961 gcacgatcgc cgtcaatccg agcctcgccg ccaatcccgc gctgctcgac ggcgtcgcca
     4021 atgtgccgag cccgaccatt gccgcggcgc tcgccgacgc tctctccttg aatacgccga
     4081 ctttcgccgc cgccggcaat atcccggcgg cgcaga
//
LOCUS       DASYNS010000502         4767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5272552, whole genome shotgun sequence.
ACCESSION   DASYNS010000502 DASYNS010000000
VERSION     DASYNS010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5272552"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VGP50_15550"
     CDS             complement(<1..1129)
                     /locus_tag="VGP50_15550"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV8028848.1"
                     /translation="MLTLSGRMGLLGTESAFEVLARAKALEAQGRSIINLGIGQPDFA
                     TPPHIVEAGRKALADGHHGYTPANGILPLREAVAADLHRRHGVSVSPDQVLVVPGGKV
                     TMFFAMMMFGEAAAEIIYPNPGFPIYESVIRFSGAVPVPMPLHEEQGFSFSAEEVLGK
                     ITPRTRLIIINSPANPTGGVVPRAELDRLVAGLERHKQVAILSDEIYGEMVYDGAEHV
                     SLLTYPAIRDRVILLDGCSKTYAMTGWRLGYGVWPKALFAAAERLAINCHSCVNAAAQ
                     YAGIAALTGPKDASQRMVAAFAERRAIIVRALNALPGFRCAVPGGAFYAFPNIVGTGL
                     DSRTLQTRLLDETGVATVSGTSFGAFGEGYLRFSYAASAGAI"
     gene            complement(1185..2225)
                     /locus_tag="VGP50_15555"
     CDS             complement(1185..2225)
                     /locus_tag="VGP50_15555"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV8028849.1"
                     /translation="MRQLPSLTPLRGIAALTVLIHHLAAGPHDPVLPRFFLRGHLGVD
                     LFFMLSGFVLAHVHLQTFLGEISWRTVGAFLWVRFARVYPMHFVVTLVLIAAGGAASL
                     SAGDVVANLVLMQVPWHVAIINEPSWSLSAELYAYLLFPFLALWLWRGDGRLAGALSF
                     LLLIALEALAVAVSGDLRGASDGWPALARGLIEFTLGVLAYRSFHDAQRSAWWQSDVA
                     LAAVVAALALAFAYSPDDGVVVAVFPLLLLAAVGNAGIATRLLNAAPLRRLGDVSYSL
                     YLAQIFAITAASGVARTSAGAALGLNGLRVLTVVAAFGISTLFYRCVEVPCRSFLRDA
                     PGRVRDMLARAA"
     gene            2569..3414
                     /locus_tag="VGP50_15560"
     CDS             2569..3414
                     /locus_tag="VGP50_15560"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEV8028850.1"
                     /translation="MPILQAMFMDWIYDHPAWLAGSVVIALAVASSWLGLVLFDRLVP
                     LSTRSRHNDVAGFIIAIVGVVYAVLLAFIAVATWESFAAANTVVAVEANYLGNLFRDT
                     IALPEPSRSEIRRELRAYLDLVIDEEWADQARGKIDPAHAWTPLERLHATLVKIHPAN
                     LGESVVQAEILRTLNELYSARRGRLLAASEGVPAIVWWIMGLGGALTVVFSFFFGVPS
                     FRMHLAMTGMLAASLALVFVLIIELDWPFRGTLSVSPDALAAVRENIERQVAGGDASS
                     GADLR"
     gene            complement(3415..3491)
                     /locus_tag="VGP50_15565"
     tRNA            complement(3415..3491)
                     /locus_tag="VGP50_15565"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3455..3457),aa:His,seq:gtg)
     gene            complement(3618..4004)
                     /locus_tag="VGP50_15570"
     CDS             complement(3618..4004)
                     /locus_tag="VGP50_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028851.1"
                     /translation="MLTRLSAPKLLKPALLGLALVPLVLSLGASPAAARGGVFLGFDF
                     VGPPAYYYPPPYYYAPPPPVVYAPPPVYAAPPVYGTAPPAAPVYQSHAGQTCREYQTT
                     VTVDGQQQPGYGTACLQPDGTWRMIN"
     gene            4076..>4767
                     /locus_tag="VGP50_15575"
     CDS             4076..>4767
                     /locus_tag="VGP50_15575"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEV8028852.1"
                     /translation="MTDRPMSPDDNALLTPAEMADADRAAMAGGVAGTALMESAGRAV
                     AQAVADRWPPRPVSVLCGPGTNGGDGFVAARHLAEAGWPVRLGLLGQRSSLKGDAAHH
                     AELWKGSVEALGPALLDGAALAIDAIFGAGLSRPVDGVARDVIEALGQRRITSIAVDV
                     PSGLDGATGEVRGVAAPAAVTVTFFRKKPGHLLLPGRALSGEIVLADIGIPAAVLGAI
                     APKTHENGPALWL"
BASE COUNT          843 a         1506 c         1699 g          719 t
ORIGIN      
        1 cgatggcgcc ggcgctcgcg gcataggaga agcgcagata gccctcgccg aaggcaccga
       61 agctggtccc cgagaccgtg gcgaccccgg tctcatcgag gagccgcgtc tggagcgtgc
      121 ggctgtcgag accggtccca acgatgttgg ggaaggcata gaaggcgccc cccggcacgg
      181 cgcagcggaa ccccgggagt gcgttcagcg cgcggacgat gatcgcgcgc cgctcggcga
      241 aggcggcgac catccgctgc gacgcgtcct ttggtccggt taatgctgca attccagcat
      301 attgcgcggc ggcattgacg caggaatggc aattgatggc gagccgctcg gcggcggcga
      361 agagggcttt cggccagacc ccatatccca gccgccaacc cgtcatcgca taggttttgc
      421 tgcagccatc gaggaggatg acgcggtcgc ggatcgcggg ataggtcagg aggctcacat
      481 gctcggcgcc gtcatagacc atctcgccat agatctcgtc gctcaagatg gccacctgct
      541 tgtggcgctc caggccggcg accaggcggt cgagctcggc cctgggcacg acgccgcccg
      601 tcggattggc cgggctgttg atgatgatga gccgggtgcg cggcgtgatc tttcccagca
      661 cttcttcggc gctgaaggag aagccctgct cctcatggag cggcatcggc acgggcacag
      721 cgcccgagaa gcggatgacg ctctcataga tggggaagcc cggattggga tagatgatct
      781 cggcggccgc ctcgccgaac atcatcatgg cgaagaacat ggtgaccttg ccgcccggca
      841 ccaccagcac ctggtcgggc gagacgctga cgccgtggcg gcgatggagg tcggcggcca
      901 ccgcctcgcg cagcggcagg atgccgttgg ccggggtgta gccgtgatga ccatcggcga
      961 gcgctttgcg gccggcctcg acgatatgcg gcggcgtggc gaaatcgggc tggccgatgc
     1021 cgagattgat gatggaacgg ccctgcgctt cgagcgcctt ggcgcgcgcc aacacctcga
     1081 aggcggattc cgtgccgaga aggcccatgc ggcccgagag cgtgagcatg agggagttta
     1141 tgccgcgcgg ggcggccgcc gaacaatagg cgatcgcgag cgcgctatgc ggcgcgcgcc
     1201 agcatgtccc tgacgcggcc cggggcgtcg cgcaggaagg agcggcaggg gacttcgacg
     1261 cagcgataga agagcgtgct gatgccgaag gctgcgacca ccgtcagcac gcgcaagccg
     1321 ttgaggccga gcgccgcgcc ggccgacgtg cgagcgacgc ccgacgcggc ggtgatggca
     1381 aagatctggg cgagatagag cgaataggag acgtcgccga ggcggcgaag cggcgctgca
     1441 ttgaggaggc gcgtggcgat tccggcattg ccgaccgccg cgagcagcag caggggaaac
     1501 accgcgacga ccacgccgtc atcgggcgaa taggcgaagg cgagcgccag cgcggcgacg
     1561 accgcggcga gagcgacgtc gctctgccac caagcgctgc gctgcgcatc gtggaaactc
     1621 cgataggcga gcacgccgag cgtgaactcg atgaggccgc gggcgagcgc cggccagcca
     1681 tcgctggcgc cgcgcaggtc gcccgatacc gcaaccgcca gcgcctcgag cgcgatcagc
     1741 agaaggaagg agagagcacc ggcgaggcgg ccgtcgccac gccagagcca gagagcgagg
     1801 aagggaaaca gcagataggc atagagctcg gcactcaacg accaggaggg ctcattgatg
     1861 atggcgacat gccagggcac ctgcatcaag accagattgg ccacgacgtc gcccgccgac
     1921 agggaagcgg cgcctccggc agcgatgagg accagcgtga cgacgaaatg catgggatag
     1981 acccgcgcaa agcgcaccca gaggaaagcg ccgaccgtgc gccaggagat ctcgccgagg
     2041 aaggtttgga gatggacatg agcaaggacg aagccgctca acatgaagaa gagatcgacg
     2101 cccagatggc cgcggagaaa gaagcgcggc agcaccgggt cgtgagggcc cgcggcgagg
     2161 tggtgaatca gcaccgtgag cgcggcgatg ccgcgcagcg gcgtcaagga cggcaattgg
     2221 cgcatcgcgc tcccacgatg gcatagccgg cttaacgatt ggtgaggata tgcgatcgcc
     2281 ggccggagcc ctcgctttcg ggcgaaattc gatgaaccac atttaatcga cacgcaaggc
     2341 gacggacagg cgcgcacaag aagccgccgc cgtggcttca tcgaggagac ttccccgcgc
     2401 ccttcctcgc cgatgcaaaa ttccgtcgcg gaggaagagc gcgcctgaaa tacgcaacgc
     2461 ggtcgatgac gagttcgcgg acgattcaaa ggtccattgc aatgcgcttt tgcggacgat
     2521 catcggccaa gccggggata gcggtcgtat acttggaatt aacgaggtat gccgatcctc
     2581 caggccatgt ttatggactg gatctacgat catccggcat ggcttgccgg cagtgtggtc
     2641 atcgcgctgg cggtggcctc ctcatggctg ggcctcgtcc tcttcgatcg cctggtgccg
     2701 ctcagcacac gcagccggca taacgacgtc gcgggtttca tcattgcgat cgtcggcgtg
     2761 gtctatgcgg tgctcctggc cttcatcgcg gtggcgacat gggagtcctt tgccgcagcc
     2821 aacaccgtcg tcgcggtcga ggccaactat ctcggcaatc tctttcgcga caccatcgct
     2881 ttgcccgagc cgtcgcggag cgagatccgc cgggagctcc gcgcctatct cgacctcgtc
     2941 atcgatgagg aatgggccga tcaggcccga ggcaagatcg atccggcgca tgcctggacg
     3001 ccgctcgagc ggctgcatgc gacgctggta aagattcatc ccgccaatct cggcgagtcg
     3061 gtggtgcagg cggagattct ccgcacgctc aacgagctct atagcgcgag gcgcggccgc
     3121 cttctggcgg cgtccgaagg ggtgcccgcg atcgtctggt ggatcatggg gctgggcggc
     3181 gcgctgacgg tcgtcttcag cttctttttc ggcgtgccga gcttccgcat gcacctggcg
     3241 atgaccggca tgctggccgc ctcgttggcc ctcgtcttcg ttctcatcat cgagctcgac
     3301 tggcccttcc gtggcacgct ttccgtctcg ccggacgccc tcgccgccgt gcgggagaat
     3361 atcgagcgcc aggtcgccgg cggcgacgca agctctggtg cagatttgag atagtggggc
     3421 gagtgaccgg gctcgaaccg gcgacatcca gaatcacaat ctggcgctct aaccaactga
     3481 gctacacccg ccaccgtggc gctggtgcta cagcgttggt gcgggggcgt caagaagagg
     3541 cttgtccgcg tgggtgcggc tctcgcgatt gctgacgcaa tcgctgacgc cgccagcgac
     3601 gacgcttcgc gtcgcgccta gttgatcatc cgccaggtgc cgtcgggctg gaggcaagcg
     3661 gtgccgtagc cgggctgttg ctggccgtcg acggtcacgg tggtctggta ctcgcggcaa
     3721 gtctggccgg cgtgggattg atagacggga gcagcgggcg gcgccgtgcc atagacgggc
     3781 ggtgccgcgt agaccggcgg tggcgcatag accacaggcg ggggtggagc gtagtagtag
     3841 ggtggtgggt agtagtaggc gggcgggccg acgaaatcga aaccgaggaa gacaccgcca
     3901 cgcgcggccg ccgggctcgc gcccaaggag agcaccagcg gcaccagcgc cagaccgagc
     3961 agcgccggtt tcaagagctt tggagccgac aggcgggtca gcatggctgt tttcccaggc
     4021 aggtaattgc gttagcgcta tatatagggg gaaagggcgt cactgcatcc cccgaatgac
     4081 cgatcgcccg atgtctcccg acgacaatgc cctgctgacc cccgccgaga tggctgatgc
     4141 cgatcgcgcc gccatggccg gcggcgtcgc cggcacggcg ctgatggaga gtgccggccg
     4201 agccgtggcc caggcggtcg ccgaccgctg gccgccgcgc ccggtgagcg tgctgtgcgg
     4261 ccccggcacc aatggcggcg acggcttcgt cgcggcgcgg cacctggcgg aagccgggtg
     4321 gccggtgcgc ctgggtctcc tcggccagcg ctcgtccctg aagggcgacg ccgcccatca
     4381 tgcggaactg tggaaaggga gcgtcgaagc gctcggaccg gcgctgctcg atggcgcggc
     4441 gctggcgatc gacgccatct tcggcgccgg gctgtcgcgg ccggtggacg gcgtcgcgcg
     4501 cgacgtgatc gaggcgctcg gccagcgccg catcacgagc atcgccgtcg atgtgccgag
     4561 cggtctcgac ggtgcgacgg gcgaggtgcg cggcgtcgcc gcaccggccg ccgtgacggt
     4621 gaccttcttt cgcaagaagc ccggccatct gctgctcccg ggccgcgctc tttcaggcga
     4681 gatcgtgctc gccgatatcg gcattcccgc cgccgtgctc ggggccatcg cgcccaagac
     4741 ccacgaaaac ggcccggcgc tctggct
//
LOCUS       DASYNS010000503         4595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_751055, whole genome shotgun sequence.
ACCESSION   DASYNS010000503 DASYNS010000000
VERSION     DASYNS010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751055"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..276
                     /locus_tag="VGP50_15580"
     CDS             34..276
                     /locus_tag="VGP50_15580"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEV8028853.1"
                     /translation="MTDAERRLWSLLRGRRLSDYKFRRQRPLGPFIADFACIEHRLVI
                     EADGGQHNETSPATAPSPSRRFASGTSLSRSSRERG"
     gene            complement(397..3111)
                     /locus_tag="VGP50_15585"
     CDS             complement(397..3111)
                     /locus_tag="VGP50_15585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8028854.1"
                     /translation="MNPLTDEADASIRDAAQQRFAGLVLDSIDDGFYVVDGEWRIVYA
                     NRRACAAWKAPAESVIGRVLWERFPQAVGSEGERHLRAAAASPGLCEFEAFSPVAERW
                     HWVRVAPLADGLVGVYWRDVTDRRNAEDALRDSKERFAAMLEALPQIAFVIRPDGTAE
                     YYNRRFTDYVGHAVGADPDSRTELQHPDDREALVQARRAGAASDNPYRVEARLRRHDG
                     AYRWHVVSNTPLRRDGKTVAWLGTAVDIDDARRAEETLRRVNEELEQRVAGRTRDLAE
                     AIARLRASEESQRALFREAPVAMHALDATRRIIDVNRRWLELLGYEHDEVIGRPIADF
                     YLAGHDSAHEARWRDLLMRGDLRQVERSFIAKSGATIDALVSVHLQLDAHQRFVRTIT
                     VVADMTARKQAEAAAQREREFSALLVENSIDAIIGLDASFRYTVWNPAMEAMSGTPKE
                     AALGRSLYELRPDIIGTPVEAAWRAALDGQRTALHDRPYQFPQSGRSGFYDADFAPLY
                     GPDGRIVGGLAFLRDITQRRLIEDQLRHAQKMEAIGQLTGGVAHDFNNIMTVIMGNLD
                     NLQRYLPDVTEPQRMIVALRRAATRAANLTHRLLAFARRQPLEPEPIDVNRLVTGMWD
                     LLRRTLGEGIAVETALPGELWRILADPNELESALLNLAVNGRDAMPMGGTLTIAAADV
                     RLDAGEAAAQDVAPGDYVLIAVSDTGIGMTPAILARAFEPFFTTKEVGQGSGLGLPQV
                     YGFVKQSGGQVKIESTPGSGTTVRLYLPRLGSGDRAGDRGGDAPETSAQRPDATILVV
                     EDDDDVRRHAVGILRELGYRVVEASDGPAALRLLGAEPRIDMLFADVVLPGGLDGHAL
                     AERARALRPELKVLLTTGYVRSTDAEAGLIPKPFTQAALADRIGRLLE"
     gene            complement(3224..3607)
                     /locus_tag="VGP50_15590"
     CDS             complement(3224..3607)
                     /locus_tag="VGP50_15590"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV8028855.1"
                     /translation="MALLALHCSVGVSTLPVAAADRSVPEDVTLVLSEYRFSPNHLVF
                     RHGVPYRLLLENRGKELHEFTAPAFFQAITLEAPESLVPGGSEVVLGPGERKALVFVA
                     AAAGRYPLSCADHDWAGMTGDIVVE"
     gene            complement(3659..4105)
                     /locus_tag="VGP50_15595"
     CDS             complement(3659..4105)
                     /locus_tag="VGP50_15595"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEV8028856.1"
                     /translation="MHRLNIIAAGALLVATGALAGCDMMGMGSNTKSLHATLSAAQEV
                     PPTKSSGTGSADFTLDPSTKQLSWKITYSGLTGDATAAHIHGPAAPGANAGVVVNLAP
                     SGLKNPIEGSTTLTDAQVGDLTSGKDYVNIHTAQNKGGEIRGQITP"
BASE COUNT          709 a         1554 c         1573 g          759 t
ORIGIN      
        1 tcgaacctgc gagcccgcac cttccgcggt gagatgaccg acgccgagcg ccgcctctgg
       61 tcgcttctcc gcggccgtcg cttgagcgac tacaaattcc gccgtcagcg cccgcttggc
      121 cccttcatcg ccgactttgc ctgcatcgaa caccgtctcg tcatcgaagc cgacggcggg
      181 cagcacaacg agacctctcc tgcaaccgcc ccctcaccct cccgacgctt cgcgtcgggc
      241 acctccctct cccgctcttc gcgggagagg ggctaattgc gtcccacttt tccctctccc
      301 gcgaagagcg ggagagggag ggacccattg cgcagcaatc ggagggtgag gggacggtac
      361 caaagcatgc actgacttcc ggcgagacta tgcttcctac tccagcaacc gcccgatccg
      421 gtcggcgaga gcggcctggg taaaaggttt ggggatcagc ccggcctcgg cgtcggtgct
      481 gcggacatag ccggtggtga ggagcacttt gagctcgggg cggagcgccc gggcgcgctc
      541 ggccagggca tggccgtcga ggccgccggg aagcacgaca tcggcgaaca gcatgtcgat
      601 ccgcggctcg gcgcccagca gccgcaaggc ggcaggtccg tcggacgcct cgaccacgcg
      661 atagccaagc tcgcgcagga tgccgacggc gtggcgccgc acgtcgtcat cgtcctcgac
      721 gacgaggatc gtcgcatccg gacgctgcgc cgaggtctcc ggcgcgtcac cccccctgtc
      781 gccggcgcga tcgccgctgc cgagccgcgg cagatagagc cgcaccgtcg tgccgcttcc
      841 gggcgtgctt tcgatcttca cctggccgcc tgattgcttc acgaagccgt agacctgcgg
      901 caggccgagg ccgctgccct gtccgacttc cttggtcgtg aagaacggct cgaaggcgcg
      961 cgcgagaatg gcgggcgtca tcccgatacc cgtatcggac accgcgatca gcacgtaatc
     1021 gccgggcgcg acgtcctgag cggcagcttc gccggcgtcg agacggacgt ccgcggccgc
     1081 aatggtgagt gtgccgccca tcggcatggc gtcgcgtccg ttgaccgcga gattgagcag
     1141 cgcgctctcg agctcgttgg ggtcggcgag gatgcgccac agctcgcccg gcagcgccgt
     1201 ttcgaccgcg atgccttcgc cgagggtgcg gcgcagcaga tcccacatgc ccgtcacgag
     1261 gcggttgacg tcgatcggct ccggctcgag cggctggcgg cgggcaaagg cgaggagacg
     1321 gtgggtcaga ttggcggcgc gggtggcggc gcgccggagc gcgacgatca tgcgctgcgg
     1381 ctccgtgaca tccggcagat agcgctgcag gttgtcgagg ttgcccatga tcacggtcat
     1441 gatgttgttg aagtcatggg cgacgccgcc ggtgagctgg ccgatggctt ccatcttctg
     1501 tgcgtggcgg agctggtcct cgataagccg ccgctgcgtg atgtcgcgca ggaaggcgag
     1561 gccgccgacg atgcggccgt cggggccgta gagcggcgcg aaatcggcgt cgtagaagcc
     1621 gctgcggccg gactgcggaa attggtacgg acggtcgtgc agagccgtgc gttggccgtc
     1681 gagcgcggcg cgccaggccg cttcgacggg cgtgccgatg atgtcgggcc gcagctcgta
     1741 gagcgatcgc ccgagcgcgg cctccttggg cgttcccgac atcgcttcca tggccgggtt
     1801 ccacaccgtg tagcggaagg acgcatcgag gccgatgatg gcatcgatgc tgttctcgac
     1861 gagcagcgcc gagaactccc gctcgcgctg ggcggccgcc tccgcctgct tgcgcgccgt
     1921 catatcggcg acgacggtga tggtccggac gaatcgctga tgcgcatcga gctgcagatg
     1981 gaccgagacg agcgcgtcga tggtggcgcc ggacttggcg atgaaggagc gctcgacctg
     2041 gcgcaggtcg ccgcgcatca gtaggtcccg ccagcgcgcc tcatgcgccg agtcgtggcc
     2101 cgcgaggtaa aaatccgcaa tcggccggcc gatgacttcg tcatgctcat agcccaggag
     2161 ctcgagccag cgccgattga cgtcgatgat gcgccgcgtg gcgtcgagcg catgcatggc
     2221 gaccggtgcc tcgcggaaca gcgcgcgctg gctttcctcg ctggcgcgca gccgcgcgat
     2281 cgcctcggcg aggtcgcgcg tgcgcccggc gacacgctgc tcgagctctt cgttgacgcg
     2341 gcgcaacgtc tcctcggcac ggcgcgcatc gtcgatgtcg acggcggtgc cgagccaggc
     2401 gacggtcttg ccgtcgcggc gcagcggcgt attgctgacg acgtgccagc gataggcgcc
     2461 gtcgtgccgg cgcagccgcg cttcgacgcg ataggggttg tcgctcgccg cgccggcgcg
     2521 gcgcgcctgc accagcgctt cgcggtcatc gggatgctgg agctcagtgc gcgaatcggg
     2581 atcggcgccg acggcatggc cgacatagtc ggtgaagcgc cggttgtaat actcggcggt
     2641 gccgtcgggg cggatgacga aggcgatctg cggcaacgcc tcgagcatgg cggcgaagcg
     2701 ctccttgctg tcgcggagcg cgtcttcggc gttgcgccgg tcggtgacgt cgcgccagta
     2761 gacgccgacc agcccgtccg cgagcggcgc cacgcgcacc cagtgccagc gctcggcgac
     2821 cggcgagaag gcctcgaatt cgcacagccc cggcgatgcc gccgcggcgc gcagatgtcg
     2881 ctcgccctcg cttccgaccg cttgcggaaa acgctcccac agcacccggc cgatcacgct
     2941 ctcggccggc gccttccagg ccgcgcaggc gcgccggttg gcatagacga tgcgccactc
     3001 gccgtcgacg acatagaagc cgtcgtcgat gctgtcgagg acgaggccgg caaagcgctg
     3061 ctgcgcggcg tcccggatgc tcgcatcggc ctcgtccgtc agcgggttca tcgccgctcc
     3121 tcgtcccggg agccggccgt cggccggacc tcccgaacca atccagagca tcctttaccc
     3181 cgaagagtag tgcaagccgg tcggagtcgc cgatccacga cgatcactcg acgacgatgt
     3241 ctccggtcat tccggcccag tcatgatcgg cgcaggacaa gggatagcgg ccggctgccg
     3301 cggccacgaa taccagcgcc ttgcgctcgc ccgggccgag cacgacctcg ctgccgccgg
     3361 gaacaaggct ctccggcgct tccagcgtga tcgcctggaa gaaggccggg gccgtgaact
     3421 cgtgcagctc cttgccgcga ttttccagga gcaagcggta ggggacgccg tgccggaaga
     3481 cgaggtggtt ggggctgaag cgatattcgg acagcaccag cgtcacgtct tccggcacgg
     3541 atcgatcagc cgccgccacc ggcagagtgg atacccccac actgcagtgc agggccagca
     3601 atgccatcag cgacccgcgc acgcgtctcc ctctcccgtg cggaaagggg acgcggtctt
     3661 acggcgtgat ctggccgcga atctcgccgc ccttgttctg tgccgtatgg atgttgacgt
     3721 agtctttgcc ggaggtgagg tcgcccacct gcgcatcggt caacgtggtc gagccttcga
     3781 tcggattctt caggccgctg ggcgcgagat tgacgaccac gccggcattg gcgccggggg
     3841 cggccggccc gtggatatgg gcggcggtcg cgtcgccggt gaggccgcta tacgtgatct
     3901 tccagctgag ctgcttggtc gatggatcga gcgtgaagtc ggcgctgccg gtgccgctgc
     3961 tcttggtcgg cggaacttcc tgggcggcgc tgagcgtcgc atgcaggctc ttggtgttcg
     4021 atcccatacc catcatgtcg caacccgcaa gcgcccctgt cgcaaccagc aacgcgcccg
     4081 cagcaataat gttcaaacgg tgcatgattc tccctccctc atggcacgcg gaaagtcggg
     4141 catgatatac cacgtgccgg tgaagccaag aagcttgcgc acgacaaagt gacgccgcca
     4201 tcgcccggct cgatgccgcg cgcgagtcgc atcacctttt gcggcgtgcc ttgcgtcccc
     4261 ctgacttgcc acgcgccgcg tcgccgatcc ggcgtcgctc gagcaactcg ccgcggtcgc
     4321 cccacgattc gcaaaagtgt cgcgtaatat actgtatttg cgggagattc tggaacggcg
     4381 cgagcggccg atgaggaccg cggcgggttg ttcgcttccg cgagatgggc taaggtgaag
     4441 cgcctttgaa cgctgccccc agccggcgtt tgcagtatct ggattcgtcc cttgaccggc
     4501 tctcccgctt cacctccgcc cttcgacatc acgccggtct ccatcgaaga ggagatgaag
     4561 cggtcgtatc tcgattacgc catgagcgtg atcgt
//
LOCUS       DASYNS010000504         7960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1372525, whole genome shotgun sequence.
ACCESSION   DASYNS010000504 DASYNS010000000
VERSION     DASYNS010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1372525"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VGP50_15600"
     CDS             complement(<1..644)
                     /locus_tag="VGP50_15600"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV8028857.1"
                     /translation="MNADNQICRMDALTLAKRIRQKEFSAIEVTEAVLRRMAVLEPHV
                     HAFCTPTPEVARAAARAVDAKIAAGDDPGVLGGVPIGIKDLVATKDILTVMGSPLYKD
                     FVPDEDDIVVERLKAAGAVIIGKTNVPEFGYSGASHNPVFPTTRNPWNLALTSGGSSA
                     GSGASVAAGVAPFAIGSDGGGSVRIPSAHCGLYGIKASMGRVPLYPGCRDERYPG"
     gene            complement(664..1626)
                     /locus_tag="VGP50_15605"
     CDS             complement(664..1626)
                     /locus_tag="VGP50_15605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028858.1"
                     /translation="MTPLLEARALTKRYPIGGRHKGAMLYAVDAVDLAIAPGECVGLV
                     GESGCGKSTLVRLLARLIDPSSGTILLDGKETTAVSQATFTTLPERARIQVVFQDPTE
                     SLNPSFRVFDAIADPLRRLLGLRDKGKLAMRVHHAADLVGLPRELIQRYPHQLSGGQR
                     ARVGIARAIAVEPSLLILDEPTSALDVSVQAVILRLLDDLRTRLGVSYLFVSHDLNVV
                     RLLCSRIIVMYLGRVVEAAPTEVLFGAARHPYTRALIGAIPDPARRGEARPRLDGSPR
                     SPIDPDPDTCRFYGRCPIATERCAAAMPELTLFGDAHVVACHFA"
     gene            complement(1623..2585)
                     /locus_tag="VGP50_15610"
     CDS             complement(1623..2585)
                     /locus_tag="VGP50_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028859.1"
                     /translation="MTALLAIEDLSLVFRTRHGTVRALEDVSLSVERGEIVGVVGESG
                     SGKSVLAYAVMGLQDAAAQVQRGSIRFGGLDVLAASEQALAAVRGRELAMIFQSPRTA
                     LNPIRKVGCQIEDILRQHAALRRSDLRRHAINALARVRIPDPERRCEAYPFELSGGMC
                     QRVMIAMALACSPFLLIADEPTTGLDVTTQAVIMDLIRDQSRSTRMSTLLITHDLGLA
                     GEYCDRIAVMHAGHLVEVAATEELLSRPAHPYTQQLLAATPTPRSTVGGLVSIPGQLP
                     DLRAELPACRFSARCRRHVPLCDERPLPWTALSPAHLVRCRVPL"
     gene            complement(2582..3445)
                     /locus_tag="VGP50_15615"
     CDS             complement(2582..3445)
                     /locus_tag="VGP50_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8028860.1"
                     /translation="MSDATLPQTLTRNVAHAFGENRLTLAAVAILLLLALCALFGNVL
                     APYDPLTTDGAARLMPPNAAHLFGTDALGRDIFSRVIVAARLDLGMAISAVVLSFLGG
                     LALGAAAGYFGGWCDRLIGRLMDTIMAFPLFVLAMGIVAALGNSVLNIIIATMIINLP
                     FYCRFARGEVNVRREMGYVEAARMGGNRSLAVLVNHIIPNILPPMMVQASLNMGWAIL
                     NAAGLSFIGLGVRPPTPEWGIMVSEGASFIISGEWWSFVFPGAALVLAVFCFNLLGDG
                     LRDLLDPRRRI"
     gene            complement(3445..4458)
                     /locus_tag="VGP50_15620"
     CDS             complement(3445..4458)
                     /locus_tag="VGP50_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8028861.1"
                     /translation="MAFVWKRLLGTLPSLAGVVVVTFLLSHALPGDPAAYFAGPAANA
                     ASIAEVRAKLGLDRSLPAQFARYLDDLAHGNLGNSLTTGQPVLTDLAQRLPASLELTL
                     FALIFAVGVAIPMGVLAATRPDSAIDHLCRASISVAAAFPTFFVGLLAVYVFYYLLDL
                     APEPVGRLNDILYAVPPRITGAYTIDSLLAGDWQVFRAALAQLILPSVSLGLFALAPI
                     ARITRAAMLQALGSDFIRTARASGLPRRKVLMTYAFRNALLPVSNVLGMVFSFLLGAN
                     VLIEQVYGWQGVGGYAVRAVLASDYAAVQGFVLMMAVLYVLLNLIVDLLGTVIDPRVR
                     FEA"
     gene            complement(4847..5239)
                     /locus_tag="VGP50_15625"
     CDS             complement(4847..5239)
                     /locus_tag="VGP50_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEV8028862.1"
                     /translation="MVKAKSLWITLAMLVAAPSMALAQDADAGAQVFAKCRICHQVGE
                     TAKIAVGPVLNGVVGRKAGTFPGYSYSAANKDSGITWDEPTLKEYLKNPRAKVPGTKM
                     IFPGLPNDQDISNVIAYLKQFGPDGKKL"
     gene            complement(5283..6164)
                     /locus_tag="VGP50_15630"
     CDS             complement(5283..6164)
                     /locus_tag="VGP50_15630"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEV8028863.1"
                     /translation="MLRAWKLRLGAATFVATLAVSTLILGHGRGSSAAVAPPDLIRLP
                     PKEFSYRANGEFTQAGRPVLAPMGTAEIARGLAIMTRQVTAAEYGRCVDAGACPRTER
                     DAGAADRPAVKVNWYDAQTYAAWLSRETGQHFRLPTDEEWSYAAADRVTDNAIVKQNN
                     PGQRAVASYEFEANLTGDLDRELRPIGSFGTNQNGLLDLAGNVWEWTDTCFDRHALDA
                     EGRTLQATSYCGIRIAEGRHRAYMLDFVRDARNGGCSVGTPPTNLGFRLVRAEDDPWH
                     GLWSLMIWAQRRLGFGA"
     gene            complement(6228..7442)
                     /gene="nirK"
                     /locus_tag="VGP50_15635"
     CDS             complement(6228..7442)
                     /gene="nirK"
                     /locus_tag="VGP50_15635"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEV8028864.1"
                     /translation="MMSRRTAVVGMAVASLVVAAPALADDLNLPRERIELVAPPFVQK
                     HEQATRQGPKIYEVRLTVEEKEMVIDDEGTKIRAMTFNGSIPAPMIVVHQDDYLEVTL
                     VNPATNTMPHNIDFHAATGGLGGGDLTLINPGEETVLRFKATRPGVFIYHCIPGGEMI
                     PWHIASGMSGAMLVLPRDGLNDGDGKPLHYDRIYYIGEQDFYVPRDKNGKFKTYESHA
                     DAFADTIETMRTLTPTHVLFEGKVGALTGKNALTAKVGENVLFVHSQADRGSNVHIVG
                     AHGDYVWEMGKFNNPPDRDVESWYVRGGSAMAMLYKFLQPGIYAYVNHNMTEAVELGA
                     TAIVKVDGPWDNDLMTQVKPPGAIEEPAMAQGSSKQPTTPKAVADAGKTPIQPAKASA
                     PAAMPEFFHVFQ"
     gene            7754..>7960
                     /locus_tag="VGP50_15640"
     CDS             7754..>7960
                     /locus_tag="VGP50_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019012698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8028865.1"
                     /translation="MHLLRVLAIAVAIVALEGAVAARAEDKVSFGTDWKAEAEHGGYY
                     QAIAAGIYRRHGIEVSLRQGGPQVN"
BASE COUNT         1396 a         2545 c         2738 g         1281 t
ORIGIN      
        1 cccggatagc gctcgtcgcg gcagccggga tagagcggca cccggcccat cgaggccttg
       61 atgccataga gcccgcaatg cgccgacggg atgcgcaccg agccgccgcc atcgctgccg
      121 atcgcaaagg gtgcgacccc cgccgccacg gaggcgcccg agcccgcgct cgagccaccg
      181 gaggtgaggg ccaggttcca cggattgcgc gtcgtcggaa agaccggatt gtggctcgcg
      241 ccgctgtagc cgaattccgg cacgttggtc ttgccgatga tgacggcgcc cgcggccttc
      301 agccgctcga cgacgatgtc gtcctcgtcg ggcacaaagt ccttgtagag cggcgagccc
      361 atgaccgtga ggatgtcctt ggtcgccacc agatccttga tcccgatcgg cacgccgccg
      421 aggacaccag gatcgtcgcc ggcggcgatc ttagcgtcga cggcgcgcgc cgcggcgcgc
      481 gccacctcgg gtgtcggcgt gcagaaggca tggacatgcg gctccagcac cgccatgcgc
      541 cgcagcacgg cctcggtcac ctcgatcgcg gagaactcct tctggcggat gcgcttggcg
      601 agcgtcagcg cgtccatgcg gcagatctgg ttgtcggcgt tcatcggcgg gttcctcggc
      661 tggtcatgcg aaatggcaag cgacgacatg ggcgtcgccg aagagcgtca gctcgggcat
      721 cgcggcggcg cagcgctccg tggcgatcgg gcaacggccg tagaaccggc aggtgtcggg
      781 atcgggatcg atcggactgc gcggactgcc gtcgagccgc gggcgcgcct cgccgcgccg
      841 cgccggatcg gggatggcac cgatcagcgc ccgcgtgtag ggatggcgtg ccgcgccgaa
      901 cagcacctcc gtcggcgccg cttcgaccac acggcccaga tacatgacga tgatgcgcga
      961 gcacagcagc cggacgacgt tgaggtcgtg cgagacgaag aggtagctca cgccgagccg
     1021 cgtccgcaga tcgtcgagca gccggaggat caccgcctga accgaaacgt cgagcgccga
     1081 ggtcggctcg tcgaggatca gcagcgacgg ctcgacggcg atggcgcggg cgatgccgac
     1141 gcgcgcgcgc tgcccgcccg acaattgatg gggatagcgt tgaatcagtt cccgcggcag
     1201 gccgacgaga tcggccgcgt gatggacgcg catggcgagc ttgcctttgt cgcgcaggcc
     1261 gaggagccgc ctcagcgggt cggcgatggc gtcgaagacg cggaaggacg ggttgaggct
     1321 ctccgtcgga tcctgaaaca ccacctgaat ccgcgcccgc tccggcaagg tggtgaaggt
     1381 cgcctgggag acggccgtcg tttcttttcc gtccagcaag atggtgccgg agctcggatc
     1441 gatgagccgc gcgaggaggc gcaccagagt cgacttgccg cagccggatt cgccgacgag
     1501 accgacgcat tctccggggg cgatcgcgag gtcgacggcg tcgaccgcgt agagcatcgc
     1561 gcccttgtgc cggccgccga tggggtagcg cttggtcagc gcccgcgctt caaggagcgg
     1621 cgtcacagcg gcacccggca acgcaccaga tgcgcgggcg acagcgcggt ccatggcagc
     1681 ggccgctcgt cgcagagcgg cacatgccgc cggcagcgtg cgctgaagcg gcaggcgggc
     1741 agctccgcgc gcagatcggg caattgtccg gggatcgaga ccagtccgcc gaccgtcgag
     1801 cgcggggtcg gcgtggcggc caggagttgt tgggtatagg gatgcgcggg ccggctcaag
     1861 agctcttccg ttgccgccac ctcgacgaga tgaccggcat gcatcaccgc gatacggtca
     1921 caatactcgc cggcgaggcc gagatcatgg gtaatcagca gcgtcgacat gcgcgtgctg
     1981 cggctctgat cgcggatcag atccatgatg acggcctgcg tcgtcacatc gaggccggtg
     2041 gtcggctcgt cggcgatgag caggaagggt gagcacgcca gtgccatggc gatcatgacg
     2101 cgctggcaca tgccgcccga cagctcgaac gggtaagctt cgcaacgtcg ttccgggtcc
     2161 gggatgcgca cgcgcgcgag cgcgttgatg gcatggcggc gaagatcgct gcggcgaagc
     2221 gcggcgtgct gacgcaggat gtcctcgatc tggcacccga ccttgcggat cgggttgagc
     2281 gcggtgcgcg ggctctggaa gatcatcgcc agctcgcggc cgcgcacagc ggcgagcgcc
     2341 tgttcagagg ccgcgagcac gtcgaggcca ccgaaccgga tgctgccgcg ttgcacctgc
     2401 gccgccgcgt cctgcagccc catgacggcg taagccaaga ccgatttccc ggagccgctt
     2461 tcgccgacca cgccgacgat ctcgccgcgc tcgacggaga gcgatacatc ctcgagcgcg
     2521 cgcaccgtgc catggcgcgt tcggaagacc agcgaaagat cctcgatggc cagcagcgcc
     2581 gtcatatccg ccgcctcgga tcgagcaggt cgcgcagccc gtcgccgagc aggttgaagc
     2641 agaagaccgc cagcacgagg gcggcgcccg gaaagacgaa gctccaccac tcgccggaaa
     2701 tgatgaagct ggcaccctcg gagaccatga tgccccattc cggcgtcggc ggccggacgc
     2761 cgaggccgat gaacgacagt cccgcggcgt tgaggatcgc ccagcccatg ttgagcgacg
     2821 cctgcaccat catcggcggc aatatgttcg ggatgatgtg gttgaccagc accgccagag
     2881 agcggttgcc gcccatgcgg gccgcctcga cataccccat ctctcgccgg acattcactt
     2941 cgccgcgcgc gaagcggcaa tagaagggca ggttgatgat catggtcgcg ataatgatgt
     3001 tgagcacgct gttgcccagc gccgcgacga tgcccatcgc cagcacgaag agcgggaacg
     3061 ccatgatggt gtccatcaag cgcccgatga ggcggtcgca ccagccgccg aaataaccgg
     3121 ccgctgcacc gagcgcgagc ccgccgagga aggagagcac gaccgccgag atcgccatgc
     3181 cgagatcgag tcgggcggcg acgatcacgc gactgaagat gtcgcgtccg agggcgtccg
     3241 tcccgaagag atgcgcggca ttgggtggca tcagtcgggc ggcgccatcc gtcgtcagcg
     3301 ggtcgtaggg cgccaggaca ttgccgaaga gggcgcacaa ggcgagcagc agaaggatcg
     3361 ccacggcggc gagggtgagc cggttctcgc cgaaagcgtg ggcaacattg cgggtgagcg
     3421 tctgcgggag cgttgcgtcg ctcatcaggc ctcgaaccgc acacgcgggt cgatgacggt
     3481 gccgagcagg tcgacgatca ggttgagcag gacataaagc accgccatca tcagcacgaa
     3541 gccttggacc gcggcatagt ccgaggcgag gactgcccgg acggcgtagc cgccgacgcc
     3601 ctgccagcca tagacctgct cgatcagcac attggcgccg agcaggaacg agaacaccat
     3661 gccgagcacg ttgctgaccg gcagcagcgc attacggaag gcataggtca tcaacacctt
     3721 gcgccgcgga aggccgctcg cgcgtgcggt gcgaatgaaa tcgctgccga gcgcctgcag
     3781 catcgccgcc cgggtaatgc gcgcgatcgg cgccaaggcg aagaggccga gcgagaccga
     3841 gggcaggatg agctgcgcca atgccgctcg gaagacctgc cagtcgcccg ccagcaggct
     3901 gtcgatcgtg taggcgccgg tgatccgcgg cggcaccgcg taaaggatat cgttgagccg
     3961 ccctacgggc tcgggcgcaa ggtcgagcag gtagtagaag acatagaccg ccagcagccc
     4021 cacgaagaag gtcggaaagg ccgccgccac cgagatcgag gcgcggcaca gatggtcgat
     4081 cgccgagtcc ggtcgggtcg cggccaacac gcccatcggg atcgccactc cgaccgcaaa
     4141 gatcagcgcg aacagcgtca gttccagtga cgccggcagg cgttgcgcca aatcggtcaa
     4201 tacgggctgc ccggtggtga gcgagttgcc gagattgccg tgcgcgagat cgtcaaggta
     4261 gcgcgcgaac tgcgcgggca ggctgcgatc gagcccaagc ttggcgcgga cctcggcgat
     4321 cgacgccgca ttcgccgccg gccctgcgaa ataggctgcc gggtcgcccg gcagggcgtg
     4381 ggatagcagg aaggtgacga cgacgacgcc cgcaaggctc ggcagagttc ccagcagccg
     4441 cttccaaacg aaggccatcg ccgcgttgcc tcactcggcg catatgtccg catgatgcat
     4501 gcaagggata tgccggtgcc ggaagacggc gcggctgcct cgcggtgctg ctctgcgatc
     4561 accgatcgcg aagacctgct caaaaagatc gacagggcgc cgataaaacc ggcaacgtga
     4621 tcacacgcca gatgcgtgca tgtgggatga gctcaagacg agcgcgcgag cgggctcatc
     4681 cgttgcggcg atttgcgcaa caattgcgag agccgcgccc atgcgggctc tcggttgtaa
     4741 aagcaaccgc tgccgctcgc gacgacgcgt tgcgtcgcgc ggggctctcg cggttgcaag
     4801 agcaaccgct gccgcccggt cgtgacaacg cttcgcgttg cgcgggctat agcttcttgc
     4861 cgtcgggccc gaactgcttc agataggcga tgacgttgct gatgtcctgg tcgttcggca
     4921 gaccggggaa gatcatcttc gtgcccggca ccttcgcccg cggattcttg agatactcct
     4981 tgagcgtcgg ctcgtcccag gtgatgccgg agtccttgtt ggccgccgag tagctgtagc
     5041 ccggaaacgt gccggccttg cggccgacga cgccgttcaa gaccggccct acggcgatct
     5101 tggcggtctc gccgacctgg tggcagatgc ggcatttcgc gaagacttgc gctcccgcgt
     5161 cggcgtcttg cgccagcgcc atgctcggcg cggcgacgag catggcaagc gtgatccaaa
     5221 gcgatttggc tttcaccatc gttttccctc agctgatttg ttggaagcgt tccggtcgac
     5281 ctctatgcgc cgaatccgag acgacgctgc gcccagatca tgagcgacca cagcccatgc
     5341 cagggatcat cctccgcgcg caccagccgg aagccgagat tggtgggcgg cgtgccgacc
     5401 gagcagccgc cattgcgcgc atcgcggacg aaatccagca tgtaggcgcg gtggcggcct
     5461 tcggcgatgc ggatgccgca atagctcgtc gcctggagcg tgcggccctc cgcatcgagc
     5521 gcatgccggt cgaagcaggt gtcggtccat tcccagacat tgccggcgag atcgaggagg
     5581 ccattctggt tggtgccgaa gctgccgatc ggccgcagct cccggtcgag atcgcccgtc
     5641 agattcgcct cgaactcata gctcgcgacg gcgcgctggc ccggattgtt ctgcttcacg
     5701 atcgcattgt cggtgacgcg gtcggcggcg gcgtagctcc attcctcgtc ggtcggcagg
     5761 cggaaatgct gtcccgtctc gcgcgagagc catgccgcat aggtctgggc atcgtaccag
     5821 tttaccttga cggcaggtcg gtcggcagcg ccggcgtcgc gttccgtccg cgggcaggcg
     5881 ccggcgtcga cgcagcggcc gtattcggct gcggtgacct gacgcgtcat gatcgcgagc
     5941 ccgcgagcga tctcggccgt gcccatcggc gccagcaccg ggcggccggc ttgcgtgaat
     6001 tcgccattcg cacgatagga gaattctttg ggcggaagcc gaatcagatc cggtggcgcc
     6061 accgcggcgc tcgagccgcg gccgtgaccg aggatgagcg tcgaaacagc gagcgtcgcg
     6121 acgaacgtcg cggcgccgag ccgaagtttc caagcgcgca gcatggggtc cctctccccg
     6181 cggtgcatga agtcctgccg gccgtgtggc cggcaggacc atcgccgtta ctggaagacg
     6241 tggaagaatt ccggcatggc tgccggcgcc gaagccttcg cgggctgaat cggggtcttt
     6301 cccgcatcgg ccacggcttt cggcgtggtg ggctgcttcg agctgccctg tgccatcgcc
     6361 ggctcctcga tcgcgccggg cggcttgacc tgcgtcatca ggtcgttgtc ccagggtccg
     6421 tcgaccttga cgatggcggt ggcgccgagc tcgacggcct ccgtcatgtt gtggttgaca
     6481 taagcgtaga tgcccggctg gaggaatttg tagagcatcg ccatggccga gccgccgcgc
     6541 acataccagc tctcgacgtc gcggtccggc gggttgttga acttgcccat ctcccagaca
     6601 taatcgccat gcgcgccgac gatatggacg ttcgagccgc gatcggcttg ggaatgcacg
     6661 aagaggacgt tctcgccgac cttggcggtg agcgcgttct tgccggtgag cgcgccgacc
     6721 ttgccttcga acaggacatg agtcggcgtc agcgtacgca tcgtctcgat cgtgtcggca
     6781 aaggcgtccg cgtgagactc gtaggtcttg aacttgccgt tcttgtcgcg cggcacgtag
     6841 aagtcctgct cgccgatata gtagatgcgg tcgtaatgga gcggcttgcc gtcgccgtca
     6901 ttgaggccgt cgcgcggcag caccaacatg gcgccgctca tgcccgacgc aatgtgccag
     6961 ggaatcatct cgccgccggg gatgcagtga tagatgaaca cgcccggccg cgtcgctttg
     7021 aagcgcagca ccgtctcctc gccgggattg atcagcgtga gatcgccgcc gccgaggccg
     7081 ccggtcgcgg catggaaatc gatattgtgc ggcatcgtat tggtggcggg attgaccagc
     7141 gtgacctcga ggtagtcgtc ctgatgcacg acgatcatcg gcgcggggat cgagccgttg
     7201 aaggtcatcg cgcgaatctt cgtgccctcg tcgtcgatga ccatctcttt ctcttcgacg
     7261 gtgagccgca cctcgtagat cttcggaccc tgccgcgtcg cctgctcgtg cttttgcacg
     7321 aagggcggcg cgacgagctc gatccgctcg cgggggagat tcagatcatc ggccaaagcg
     7381 ggggccgcga caacgaggct cgcgacagcc atgccgacca ctgctgtccg tctgctcatc
     7441 atgcaaactc tccttcggtt ttttggcccc ggctgcagcc gcttctctgc acggcaaaac
     7501 acaccggcgc gaatccctgg ccgatagtgg gacgggactc tacacaatta tcgtgccgtt
     7561 tcaatcacat ttcatgggaa tcgaaaaaat ttcatgcaac gtgccgttgc ccgtcgaccg
     7621 gcgcgggtgg tctgtacatt gttattattc taaaagggtt tctggcgcct tggcagcggc
     7681 cggttttgag ttaggagccc cgccagggct cggctagaac ccctggtgag ggagaaatta
     7741 gggaggagac cctatgcact tgctgagagt gctcgctatc gccgttgcca tcgtggcgct
     7801 tgaaggcgcg gtcgcggcgc gcgccgagga caaggtgagc ttcggcaccg actggaaggc
     7861 cgaggcggag catggcggct actatcaagc gatcgccgcc gggatctatc gccggcacgg
     7921 catcgaggtc agtctgcgcc agggcgggcc gcaggtgaat
//
LOCUS       DASYNS010000505         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3715572, whole genome shotgun sequence.
ACCESSION   DASYNS010000505 DASYNS010000000
VERSION     DASYNS010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3715572"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VGP50_15645"
     CDS             complement(<1..763)
                     /locus_tag="VGP50_15645"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8028866.1"
                     /translation="MSEPAARAPRRLSDTLAVYLKRRVLVVAFLGFSSGLPLALSGST
                     LLVWMREVGVDLGTIGLFALVGTPYTVKFLWAPLVDALDVPVLSRRFGRRRGWLLLAQ
                     FLLIAAIVLLGACDPATSAPIVALGALLVAAASATQDIVVDAFRVESLDESEQAAGMA
                     SYVAAYRIGMLASTAGALFLVSGFESFLGLSRHGAWSAGYAVMAALVLVGVATTLFAA
                     EPERSVAAAAAHEAAVAHENPLQRIITAAAGAFAEF"
     gene            complement(760..1875)
                     /gene="rmuC"
                     /locus_tag="VGP50_15650"
     CDS             complement(760..1875)
                     /gene="rmuC"
                     /locus_tag="VGP50_15650"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV8028867.1"
                     /translation="MVENPTLIAILAVLVACGALAALAIVLYRARRPARREEDEMAAL
                     ARQQTDAAARLEGMIKLLANGQSQLQRAVNDRLDSVSSRLGDSMEKTKQNTAENLQKL
                     NERLAVIDRAQKNITELATQVTSLQSVLANKQSRGAFGQGQMEAIIQDGLPKGAYAFQ
                     QSLSNRTRPDCCVFLPDRRPLVIDAKFPLEAVTAHREAKSDDERKAAAQRLRTDVAKH
                     VSDIAGKYLIPGETQELAFMFVPSESVYAELHDGFDDIVQKAFRAKVVIVSPSLLMIA
                     IHVIRQIQKDARMREAAGEIRDEVGHLMKDVGLLGERVRKLQAHFTQSNEDIRQALIS
                     VDKIEARGERIREVELGEAAPGANMIAGQVRRLEAGE"
     gene            2016..2540
                     /locus_tag="VGP50_15655"
     CDS             2016..2540
                     /locus_tag="VGP50_15655"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV8028868.1"
                     /translation="MTVREIVKLPDKRLRLVSEPVKRIDAGVRKLVEDMFATMYAAPG
                     IGLAAIQVGVAKRVVVMDLSKREDEHKPLAFINPEITWTSEEKATHEEGCLSIPEYYE
                     EVERPERVKVKYLDLDGKAHEVTAKGLFATCIQHEVDHVNGVLFIDHISKLKRDRVTK
                     KFIKAAKTAKKVEA"
BASE COUNT          479 a          872 c          880 g          419 t
ORIGIN      
        1 ggaattccgc gaaagcgccg gcggcggcgg tgatgattcg ctgcagcggg ttttcgtgtg
       61 cgacggcggc ctcatgcgcc gccgccgccg cgaccgagcg ctccggctcg gcagcgaaga
      121 gcgtcgtcgc gaccccgacc aggacgagcg ccgccatcac cgcatagccg gcactccagg
      181 cgccgtggcg gctcaagccg aggaaggatt cgaagccgct caccaggaac agcgcgccgg
      241 cggtcgacgc gagcatgccg atgcggtagg cggcgacata ggacgccatg cctgcggcct
      301 gttcgctttc gtccaggctt tcgacgcgga acgcatcgac gacgatgtcc tgcgtcgccg
      361 aagcggcggc gacgaggagc gcgccgagcg ccacgatcgg cgccgaagtg gccggatcgc
      421 aggcgccgag caagacgatc gcggcgatga gcagaaactg cgccagcaac agccagccgc
      481 ggcgccggcc gaagcgccgc gacagcaccg gcacatcgag cgcgtcgacc agcggcgccc
      541 agaggaattt caccgtatag ggcgtgccga cgagggcaaa gaggccgatg gtgccgagat
      601 cgacgccgac ctcccgcatc cacaccagaa gcgtcgagcc ggacaaagcg agcggcaggc
      661 cggaggaaaa gccgagaaaa gcgacgacga ggacgcgccg cttgagatag acggcgagcg
      721 tgtctgatag ccgccgcgga gcgcgggcgg caggctcgct cactcgccgg cctccagcct
      781 gcgcacctgc cccgcgatca tgttcgcgcc cggcgccgct tcgcccagct cgacttcacg
      841 aatgcgctcg ccgcgcgcct cgatcttgtc gaccgagatg agcgcctggc ggatatcctc
      901 gttcgattgg gtgaaatggg cttgcagctt gcgcacgcgc tcgccgagca ggccgacgtc
      961 cttcatcaga tgcccgactt cgtcgcggat ctcgccggcg gcctcgcgca tgcgcgcgtc
     1021 cttctggatc tggcggatga cgtggatggc gatcatcagc agcgacggcg agacgatgac
     1081 caccttggcg cggaacgcct tctgcacgat gtcgtcgaag ccgtcatgca gctcggcata
     1141 gaccgattcc gacggcacga acatgaacgc cagctcctgg gtctcgccgg gaatgaggta
     1201 tttcccggcg atgtcgctga catgcttggc gacgtcggtg cgcagccgct gcgccgcggc
     1261 cttgcgctcg tcgtccgact tggcctcgcg atgagcggtg accgcctcga gcggaaattt
     1321 cgcatcgatc accagcggcc gccggtcggg caggaacacg cagcaatcgg gccgggtgcg
     1381 gttcgacagc gactgctgaa aggcataagc gcctttcggc aagccgtcct ggatgatcgc
     1441 ttccatctgt ccctggccga aggcgccgcg cgactgcttg ttggcgagca cgctctgcag
     1501 cgaggtgacc tgcgtggcga gttcggtgat gttcttctgc gccctgtcga tcaccgccag
     1561 ccgctcgttg agcttttgca ggttctccgc cgtgttctgc ttggtctttt ccatggaatc
     1621 gccgagacgg ctggaaaccg aatcgagacg gtcattgacc gcgcgctgca gctgcgactg
     1681 gccgttggcc aggagcttga tcatgccttc aagtcgcgcc gcggcgtcgg tctgctgccg
     1741 tgcgagcgcg gccatctcgt cttcttcccg gcgcgccgga cggcgcgcgc gatagagcac
     1801 gatcgcgagc gccgcgagcg caccgcaagc gacaaggaca gccaaaatcg cgatcagggt
     1861 agggttttcg accatgcgct gcttctacgc gattcgctgc ggtgacaggg gattacgcta
     1921 gaacgaatag ggaacatcct taacgcatcc acaccggtcc gcgcaaccgg cagcgcgcat
     1981 tgaccgggct ttacgcaccg cctacatcgc cgcccatgac cgtgcgcgag atcgtcaagc
     2041 ttcccgacaa gcggctgcgg ctcgtgtccg agccggtcaa gcgcatcgat gccggcgtgc
     2101 gcaaactggt cgaggatatg ttcgcgacca tgtatgccgc gcccggcatc gggctcgccg
     2161 ccatccaggt cggcgtcgcc aagcgcgtcg tggtcatgga cctgtcgaag cgggaggacg
     2221 agcacaagcc gcttgcgttc atcaatccgg agatcacctg gacgtcggaa gagaaggcca
     2281 cccacgagga gggctgcctg tcgatcccgg aatattacga ggaagtggag cggccggagc
     2341 gggtcaaggt caaatatctc gaccttgacg gcaaggcgca cgaggtcacg gccaagggcc
     2401 tgttcgccac ctgcatccag cacgaggtcg accacgtcaa cggcgtcctg ttcatcgatc
     2461 atatctccaa gctcaagcgc gaccgcgtta ccaagaaatt catcaaggca gcgaagacgg
     2521 cgaagaaggt ggaggcgtga ttggcgctcc gcctggtgtt catgggcacg cccgacttcg
     2581 ccgtgcccac gctggtcgag atcgtcgccg gcggccataa ggtcgccgcg gtctataccc
     2641 gcgccccgca
//
LOCUS       DASYNS010000506         3587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4339298, whole genome shotgun sequence.
ACCESSION   DASYNS010000506 DASYNS010000000
VERSION     DASYNS010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4339298"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VGP50_15660"
     CDS             complement(<1..780)
                     /locus_tag="VGP50_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8028869.1"
                     /translation="MSASPEEQASGDRSRLLTEQAAPGIELTGNEAKYHAIIDTAVDA
                     IAVIDEAGVVQSFNLAAQKIFGYAAAAVVGRNVKMLVPEPDRSAHDGYIESYRRTGKA
                     KTIGVFREVQGQRSDGTLFPLELSIAEWRAGGKRYFTGVMRDVTERKRAEEALRRLNE
                     TLEQRVLERASALEDANRRLTSQMETLQRAQMALQQAQKMEAIGQLTGGIAHDFNNLL
                     TAIAGNLDLVAAGIGNNQSLHRLIDAAQRAAQRGGRLTQQLL"
     gene            980..1321
                     /gene="arsC"
                     /locus_tag="VGP50_15665"
     CDS             980..1321
                     /gene="arsC"
                     /locus_tag="VGP50_15665"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538864.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEV8028870.1"
                     /translation="MDVTIYHNSRCAKSRETLALLERRGLKPKVIEYLKTPPDRAELK
                     RLLKLLGKPPRALLRAKEAREAGLDKPGLSDEAIIAGMVAHPGVIERPIVVTPRGAAL
                     GRPPEAVLDIL"
     gene            complement(1327..1950)
                     /locus_tag="VGP50_15670"
     CDS             complement(1327..1950)
                     /locus_tag="VGP50_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028871.1"
                     /translation="MMALSETWEKLCKDLKIPCGAVFEVIKERYGEAHRFYHGIAHVE
                     SLLKLLHDDFPTLGKDRAAIELALWFHDIVFDPHARDNEEKSVELMRSVIGKTLAPAL
                     ERKVEDFILATKHDGPVTDPDAQLVVDIDLSTLGAPPEDFQRYSESIRKEYFWKPEAE
                     YRQGRKATLLRFLNQSPLYHHLPLRHRFEAPAKRNLTEALAELRDVP"
     gene            2137..3342
                     /locus_tag="VGP50_15675"
     CDS             2137..3342
                     /locus_tag="VGP50_15675"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8028872.1"
                     /translation="MRNDHVLWAPALLALALATPAAAQKQYGPGVGDDEIKIGQTMPF
                     SGPASAYSTIAKAELAYFAMVNEQGGVNGRKIRLISLDDGYSPPKTVEQTRRLVEQEN
                     VLLDFNPLGTASNSAIQKYLNENKVPQLFVATGATKWGDPVHFPWTMGWAPTYQTEGH
                     IFARFILAHAPEAKIAVLYQNDDYGKDYVKGLKDGLGHKAASLIVKEASYETSDPSID
                     SQIVSLQASGATVFYDVSGPKFAAQAIRKVYDIGWHPLHLLNSVSGSVGAVLKPAGFE
                     RSEGIITAIYGKDPTDPQLQEDPAFKDWLAWMQHYYPEGDLTDAANVYGYNVAMTLVQ
                     VLKQCGDEVTRENVMRQAAHLDLELPMLLPGVKVNTGPTQFYPIREMQLVRFNGKVYD
                     RVGEVILSE"
     gene            complement(3371..>3587)
                     /locus_tag="VGP50_15680"
     CDS             complement(3371..>3587)
                     /locus_tag="VGP50_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV8028873.1"
                     /translation="PFNPWPGAPFERSTAAAIKLFSDIVNAAGYSSPVRTPRGEDIMA
                     ACGQLKSDSVRRRKSAPASEAAAAPTA"
BASE COUNT          616 a         1182 c         1180 g          609 t
ORIGIN      
        1 gaggagctgc tgggtcagcc gcccgccgcg ttgcgccgcg cgctgcgccg cgtcgatcag
       61 gcgatgcagg ctctgattgt tgccgatgcc ggcggcgacg aggtcgagat tgccggcgat
      121 cgccgtcagc aggttgttga agtcatgcgc gatgccgccg gtgagctggc cgatcgcctc
      181 catcttctgc gcctgctgca gcgccatttg cgcgcgctgc agcgtctcca tctgggaggt
      241 gagccgccgg ttggcgtctt cgagcgcgct ggcgcgctcg agcacgcgct gctcgagcgt
      301 ctcgttgagc cgccggagcg cctcttcggc gcgcttgcgc tcggtgacgt cgcgcatcac
      361 gccggtgaag tagcgcttgc ccccggcgcg ccattcggcg atcgacagct cgagcgggaa
      421 gagcgtaccg tcgctgcgct ggccctgcac ctcgcggaag acgccgatgg tcttggcctt
      481 tccggtccgg cgatagctct cgatgtagcc gtcatgcgcc gagcggtcgg gctccggcac
      541 cagcatcttg acgttccgcc cgaccaccgc cgcggcggca tagccgaaga tcttctgggc
      601 ggcgaggttg aaggactgca cgacgcccgc ctcgtcgatg accgcgatcg cgtcgaccgc
      661 ggtgtcgatg atggcgtggt atttggcctc gttgccggtc agctcgatgc ccggcgccgc
      721 ctgctcggtc aggagccggg agcgatcgcc cgaggcttgc tcttccggtg acgcgctcat
      781 acccgcgttc cactcagcgc atgatcccgc gctcgacacc aagttttgaa aaactctacg
      841 cgccggcacc gatgatcact agaggtaaat gccagttttg caaccgaaaa ggcggggacg
      901 cccgggggat cttggccgat ccagcgaaag ttctatgatc cggagtgtgg atcggcgagg
      961 aagagggctc acccgcgaca tggacgtcac catctatcac aattcccgct gcgcgaagtc
     1021 gcgcgagacc ctggcactgc tggagcgccg cggcctcaag cccaaagtca tcgagtatct
     1081 gaaaacgccg cccgacaggg ccgagctgaa gcggctcttg aagctgctcg gcaagccgcc
     1141 gcgcgcgctg ctccgggcta aggaggcgcg cgaagccggg ctcgacaagc cggggctcag
     1201 cgatgaggcg atcatcgccg ggatggtcgc gcatcccggt gtcatcgagc ggccgatcgt
     1261 cgtgacgccg cgcggcgcag ccctcggccg tccgcccgag gcggtgctcg atattctctg
     1321 aaccggctac ggcacgtctc tgagttccgc cagcgcctcg gtgagattgc gctttgccgg
     1381 cgcctcgaag cggtggcgca gcggcaagtg gtgatagagc ggggactggt tgagaaagcg
     1441 cagcagcgtg gccttgcggc cctggcggta ctcggcctcg ggcttccaga agtattcctt
     1501 gcggatgctc tcgctgtagc gctgaaaatc ctcgggcggg gcgcccagcg tcgagagatc
     1561 gatatcgacc accagctgcg cgtcggggtc ggtcaccggc ccgtcatgct tggtggcgag
     1621 gatgaagtcc tcgaccttgc gctcgagcgc cggcgcgagc gtcttgccga tgacgctccg
     1681 catcagctcc acgctcttct cctcattgtc gcgggcgtgc ggatcaaaga cgatgtcgtg
     1741 gaaccagagc gccagctcga tcgccgcccg gtccttgccg agcgtcggga aatcgtcgtg
     1801 gagcagcttc agcagcgact ccacatgggc gatcccgtga tagaaacggt gcgcctcgcc
     1861 atagcgctct ttgatcacct cgaaaacggc gccgcacggg atcttcaggt ccttgcagag
     1921 cttctcccag gtctcgctca acgccatcat gttcacccgc gtccggcagc ggcgtcctgt
     1981 cgatggacat agtgtaagac ggcggcgcga tcctcggcaa atcgccccgc catcgcgcca
     2041 ttgacgcccc tagcgtcccg taggaccatg gccgtgtcga gtaacaaaaa agaaacgcac
     2101 cggaattttc ccgggcggga gacagggaga aagcgaatgc ggaatgacca tgtcctgtgg
     2161 gcgccggcgc tgctggcgct ggcgctggcg acgccggcgg ccgcgcagaa gcaatacggc
     2221 ccgggcgtcg gcgacgacga gatcaagatc ggccagacca tgcccttcag cgggcccgcc
     2281 tcggcctaca gcaccattgc caaggccgag ctggcctatt tcgctatggt gaacgagcag
     2341 ggcggagtca acgggcggaa gatcaggctg atatccctcg atgacggcta tagcccgccc
     2401 aagacggtgg agcagacccg ccgcctggtc gagcaggaga acgtgctgct cgacttcaac
     2461 cccctgggca cggcctccaa cagcgccatc cagaaatatc tcaacgagaa taaggtgccg
     2521 cagctcttcg tcgccaccgg cgccaccaaa tggggcgatc ccgtgcattt cccctggacc
     2581 atgggctggg cgcccaccta ccagaccgag ggccacatct tcgctcgctt catcctcgcc
     2641 cacgcgcccg aggccaagat cgccgtgctc tatcagaatg acgattacgg caaggactat
     2701 gtgaagggtc tgaaggacgg tctcggccac aaggccgcca gcctgatcgt caaggaggcg
     2761 agctatgaga ccagcgaccc gagcatcgat tcgcagatcg tgtcgcttca ggcctccggg
     2821 gcgacggtct tctacgatgt gagcggcccg aaattcgccg cgcaggcgat ccgcaaggtc
     2881 tacgacatcg gctggcaccc gctgcatctc ctcaactcgg tctcgggctc ggtcggcgcc
     2941 gtgctcaagc cggcgggctt cgagcgctcg gagggcatca tcaccgccat ctacggcaag
     3001 gatccgaccg atccgcagct gcaggaggat cctgccttca aggactggct cgcctggatg
     3061 cagcactact atcccgaagg cgatctgacc gacgccgcca acgtctatgg ctataacgtc
     3121 gccatgacgc tcgttcaggt gctgaagcaa tgcggcgacg aggtcacgcg cgagaatgtg
     3181 atgcggcagg cggcgcatct cgatctcgag ctgccgatgc tgttgcccgg cgtcaaagtc
     3241 aacaccggcc cgacgcagtt ctatccgatc cgcgagatgc aactcgtccg cttcaacggc
     3301 aaggtctacg accgcgttgg cgaggtgatc ctctcggaat agccggcgcc tcgcgacgag
     3361 ggccgcgcgc tcacgccgtg ggcgccgcgg ccgcttcgct cgcgggcgcg cttttgcggc
     3421 gccggacact gtcggatttg agctgaccgc aggcggccat gatgtcctcg ccgcgcggcg
     3481 tgcgcaccgg cgacgaatag ccggccgcgt tgacgatgtc ggagaagagc ttgatcgccg
     3541 cggcggtcga gcgctcgaag ggcgcgcccg gccagggatt gaacggg
//
LOCUS       DASYNS010000507         4324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1690663, whole genome shotgun sequence.
ACCESSION   DASYNS010000507 DASYNS010000000
VERSION     DASYNS010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690663"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VGP50_15685"
     CDS             <1..345
                     /locus_tag="VGP50_15685"
                     /inference="COORDINATES: protein motif:HMM:NF025888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEV8028874.1"
                     /translation="ERITGRSDDMLIIRGVNLFPSQIEELILKEPTLSGHYVLELSRP
                     KALDELKVVAEFRPEAAANSVAVVDRVAARLQHDIKSMIGITAEVEIAPPGRVERSIG
                     KAKRVIDRRPQR"
     gene            426..1304
                     /locus_tag="VGP50_15690"
     CDS             426..1304
                     /locus_tag="VGP50_15690"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEV8028875.1"
                     /translation="MIRLRKLLFALIWALIVLSPALADARAGGSARPGGSMGYSSSPS
                     IGSRSTPSSPSSPSYAPAPGFAQSHPFLTGLAGGFFGSWLGSLLFPHWGGYGYGVGGS
                     LFSWLIIIGLFWFLFRMFRGPTGPLTSPPYFDRSAASAAAPLGYGAVARRGTLSVTDT
                     DYAAFEAILKTVQGAWSKGDLGTLRHYVTPQMLEYFSNALANNASQGVENHVEQVELV
                     KGDVREAWDEGQTHYATCLLHWRAIDYTTRAGEPGSIVEGDPQRPADEQELWTFSRSP
                     GGHWLLAAIEQVSTVR"
     gene            1543..1917
                     /locus_tag="VGP50_15695"
     CDS             1543..1917
                     /locus_tag="VGP50_15695"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV8028876.1"
                     /translation="MPLTQLNHVTVRTDDLEGTRDFYHAVLGLAPGPRPPLAFPGYWL
                     YCGESPVVHLVPRSGAIGAGDGGDTGNFDHVAFNARDFDGMRARFKELGIKFNEREVP
                     GIKLRQLFVQDPNKVMIEINFP"
     gene            2030..2188
                     /locus_tag="VGP50_15700"
     CDS             2030..2188
                     /locus_tag="VGP50_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028877.1"
                     /translation="MSILMTSMGWAILMVGLALAGDVAWTAYREWRMRRDAEKNGTTL
                     TTKYGLPH"
     gene            complement(2217..3389)
                     /locus_tag="VGP50_15705"
     CDS             complement(2217..3389)
                     /locus_tag="VGP50_15705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8028878.1"
                     /translation="MTSSPPAAPGDDIAAIRQAVAALCAHFPGEYWRTLDRERAYPGD
                     FVRALTEAGFLACLVPEAYGGSGLGIAAAAAILEEVQKSGGNSAACHAQIYIMGTLLR
                     HGSAAQKAAWLPRIAKGELRLQAFGVTEPSAGTDTSAIRTTATRQGDHYVVNGQKVWT
                     SRAEHSDLMLLLARTAPRDERRRTEGMSVFLVELRAALGKGVAIRPIRTMMNHATTEI
                     FFDDLTVPAANLIGEEGKGFRYILDGMNAERILIAAECVGDARWFIAKASDYAKSRVV
                     FGRPIGQNQGVQFPIARAFAASEAAALMVERAAALYDAGEPCGKEANMAKLLAADASW
                     QAADMCVQTHGGFGFAEEYDIERKFRETRLYQVAPVSTNLILAYLAEHVLGLPRSY"
     gene            3658..>4324
                     /locus_tag="VGP50_15710"
     CDS             3658..>4324
                     /locus_tag="VGP50_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028879.1"
                     /translation="MSSRAEETRRAWLAVIERHRHDRDAAGSERYWSPRLDCASRDEI
                     RAIQDEKVAAVVPFLYENSPFYRRRFDRLALGPSDIRNVDDLVAKWPVLDRAEMAEDA
                     REHPPYGTYTAMTDAVWAERGWMMFSSSGTTDMPRVFRYSHVDRELWAWANARALHAM
                     DLRRGDTVFVISGLGPHVWAWAVHSALMKMRLPWLSGGGMDAKARATVVLRYRPTILL
                     CTPS"
BASE COUNT          712 a         1477 c         1413 g          722 t
ORIGIN      
        1 gagcgcatca ctggccgcag cgacgacatg ctcatcatcc gcggcgtcaa cctgttcccg
       61 agccagatcg aggagctgat cctgaaagag cccacgctct ccggccatta tgtgctggag
      121 ctgagccggc ccaaggcgct cgacgagctg aaggtggtcg cagagttccg ccccgaggcg
      181 gcagccaaca gcgtcgcggt cgtcgaccgc gtcgccgccc ggctccagca cgacatcaaa
      241 tcgatgatcg gcatcaccgc cgaggtcgag atcgcgccgc ccggccgcgt cgagcgctcc
      301 atcggcaagg ccaagagagt gatcgaccgt cgcccgcagc ggtgatcccg cagccttgca
      361 gacgccgcgt cgggccacga aataacagag gtccggctgt cgtcaaagcg cccgaggaac
      421 caatcatgat caggttgcgg aagttgctgt tcgcgctcat ctgggcgttg atcgtgctgt
      481 cgcctgcgct ggccgacgcg cgcgccggcg gcagcgcgcg tccgggcggg agcatgggct
      541 attcgagctc tccgagcatc ggatcgcgca gcacgccgtc gagcccgagc tctccgtctt
      601 acgcgccggc gccgggcttt gcgcaaagcc atcccttcct caccgggctt gccggcggct
      661 tcttcggctc ctggctcggc agcctgctct tcccgcattg gggcggctac ggctacggtg
      721 tcggcggctc tctcttctcg tggctgatca tcatcggcct cttctggttc ttgttccgga
      781 tgttccgggg accgacaggg ccgctgacct cgccacccta tttcgaccgt tccgcggcga
      841 gcgctgccgc tcctctgggc tatggcgctg ttgcgcggcg cggcacgctc tccgtcaccg
      901 acaccgacta cgccgccttc gaagcgattc tgaagacggt gcagggcgcc tggagcaagg
      961 gcgacctggg cacgctgcga cactatgtga cgccgcagat gctcgaatat ttcagcaacg
     1021 cgctcgccaa caacgcgagc caaggggtcg agaatcacgt cgagcaggtc gaactcgtga
     1081 agggcgacgt ccgcgaggcc tgggacgagg ggcagacgca ctacgcgacc tgcctcctcc
     1141 attggcgcgc catcgactac acgacgcgcg cgggcgagcc cggctcgatc gtggaaggcg
     1201 atccgcaacg gccggcggac gagcaggagc tatggacctt ctcccgcagc cccggcggcc
     1261 actggctcct cgccgcgatc gagcaggtaa gcacggtcag atagagcgag ccatctcgcc
     1321 ctctcccgtg gcgagcggga ggggcgtgcg cgaagcccgc gccggggcgt cgacgacgga
     1381 gccgtgcttt cgcccttccc tcaccctccc ggcgctgtgc gcctgggacc cttcccttgc
     1441 cgctcttcgc gggagaggtc aaattagcga catcaatcat cagcggtctg cacaaatccc
     1501 gcagatgccg ctattgtcgg cggcaaagct cggaggagca cgatgccgct cacgcagctc
     1561 aatcacgtca cggtgcgcac cgacgacctc gagggcacgc gcgacttcta tcatgcggtg
     1621 ctcggtcttg cgccgggacc gcgcccgccg ctggccttcc caggctattg gctctactgc
     1681 ggcgagagtc ccgtcgtgca tctcgtgccg cggagcggcg ccatcggtgc gggcgacggc
     1741 ggcgacaccg gcaacttcga ccatgtggcc ttcaacgccc gggatttcga cggcatgcgc
     1801 gcacgcttca aggagctcgg catcaaattc aacgagcgcg aggtgccggg gataaaactg
     1861 cgccaactct tcgtacaaga tccgaacaag gtgatgatcg agattaactt tccgtaattc
     1921 gctcgccgcg ccgttgcaat ttgtcacgtt tgggcgcggc gggacacaga aactaaaccg
     1981 cgtggttgag tgtttgctcg acgagcggga agtgttgagg gcacgtggca tgagtatcct
     2041 catgacgagt atgggatggg cgatcctgat ggtcggactg gcacttgccg gcgacgtcgc
     2101 ctggacggcg taccgcgagt ggcggatgcg gcgcgacgcc gagaagaacg gcaccacgct
     2161 cacgaccaaa tacgggctgc cgcactgaca cacgggcaca gccgcccgcg cccgcttcag
     2221 taggagcgcg gcaatcccag gacatgttcg gccagatagg ccaggatgag attggtcgag
     2281 accggcgcca cctgatagag ccgcgtttcc cggaacttgc gctcgatgtc gtattcctcg
     2341 gcgaagccga agccaccatg ggtctgcacg cacatatcgg ccgcctgcca tgaggcgtcc
     2401 gccgccagca gcttcgccat gttcgcttcc ttgccgcagg gctcgcccgc gtcgtaaagc
     2461 gccgcggcgc gctcgaccat gagggcggcg gcctcgctcg ccgcaaaagc gcgcgcgatc
     2521 gggaactgca cgccctggtt ctggccgatg ggacggccga acaccacgcg gctcttcgcg
     2581 tagtcgctgg ccttggcgat gaaccagcgc gcatcgccga cgcattcggc ggcgatgagg
     2641 atgcgctcgg cattcatgcc gtcgaggatg tagcggaagc ccttcccttc ctcgccgatg
     2701 agattcgccg ccggtacggt gagatcgtcg aagaagatct cggtcgttgc gtggttcatc
     2761 atggtgcgga tcgggcgaat cgcgacgccc ttgcccagcg cggcgcgcag ctcgacgagg
     2821 aacaccgaca ttccctcggt gcgccggcgc tcgtcgcgcg gcgcggtgcg cgccagcagc
     2881 agcatgagat cggaatgctc cgcgcgcgag gtccagacct tctggccgtt gacgacgtag
     2941 tggtcgccct gccgtgtcgc ggtcgtccgg atcgccgagg tatcggtgcc cgccgacggc
     3001 tcggtcacgc cgaaggcctg gaggcggagc tcgcctttgg cgatccgggg gagccacgcc
     3061 gctttctgcg ccgcgctgcc atggcggagt agcgtcccca tgatgtagat ctgcgcatgg
     3121 caggccgcgc tgttgccgcc gctcttctgc acttcctcga ggatggcggc cgccgcggcg
     3181 atgccgagac cgctaccgcc ataggcttcg ggcacgaggc aggcgaggaa gcccgcctcg
     3241 gtaagtgcgc ggacgaaatc cccgggatag gcgcgctcgc ggtcgagcgt ccgccaatac
     3301 tcgccgggga aatgcgcgca gagcgccgcc acggcctgac ggatggcggc gatgtcgtcg
     3361 ccgggcgcag caggcggcga agatgtcatt gagctctgac ctcgaccatc ggcaactggt
     3421 aagaccatcg ccgcagtcat tgccgcatcg accgctcctt gtacagaggc ggccctcgcc
     3481 ctccgccggt acgggaaagg gtttcctcgc gatatcaggc cccctctcca gcgtcggccg
     3541 agggacgcat aaaggtaaaa gctcagcttg accgatcatc cgccgcacca cataaatctg
     3601 tcgacaccgg cggcgggctt gaagagaatg gaggccctgg acgacgctgg gacgctcatg
     3661 tcttcacgcg cggaagagac ccggagagcc tggctcgcgg tcatcgagcg ccatcgccac
     3721 gaccgcgacg ccgcgggatc cgagcgctac tggtcgccgc gtctcgactg cgcatcgcgc
     3781 gacgagatcc gcgccatcca ggacgagaag gtcgcagcgg tcgtgccctt cctctacgag
     3841 aacagcccct tctaccgccg ccggttcgac cggctcgcgc tcggtccgtc cgacatccgc
     3901 aacgtcgacg acctcgtcgc gaaatggccg gtcctcgacc gcgccgaaat ggccgaggat
     3961 gcgcgcgagc acccgcctta tggcacctac accgcgatga ccgacgcggt ttgggcggag
     4021 cgcggctgga tgatgttctc gtcctcgggc accaccgaca tgccgcgcgt cttccgctat
     4081 tcgcatgtgg atcgcgagct ctgggcctgg gccaatgctc gcgccctcca tgccatggat
     4141 ttacgccgcg gcgacaccgt tttcgtcatc agcggcctcg gcccccatgt ctgggcctgg
     4201 gccgtccatt cggcgctgat gaagatgcgg ctgccctggc tctcgggcgg cggcatggac
     4261 gccaaggcgc gcgccaccgt cgtgctgcgc tatcgcccga cgatcttgct ctgcaccccc
     4321 tcct
//
LOCUS       DASYNS010000508        14835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2156561, whole genome shotgun sequence.
ACCESSION   DASYNS010000508 DASYNS010000000
VERSION     DASYNS010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 14835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14835
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2156561"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VGP50_15715"
     CDS             complement(<1..303)
                     /locus_tag="VGP50_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028880.1"
                     /translation="MTVNVAATPFTRIAKRGLGQAIAAAIRGGDLATDFRAAICRFED
                     DLFAEFPAGHGLMDYDAAAAMLREVFAACGAAPPALALVPGFDDPRVGGFADVANHR"
     gene            complement(371..604)
                     /locus_tag="VGP50_15720"
     CDS             complement(371..604)
                     /locus_tag="VGP50_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028881.1"
                     /translation="MSMWRRRLGPDDNYGITIGQRFSPVGRAKAVWEVITVARYPGDV
                     APHARLSRVGAQHEGKTLSLQVLLDRRFYQPTQ"
     gene            complement(668..1456)
                     /locus_tag="VGP50_15725"
     CDS             complement(668..1456)
                     /locus_tag="VGP50_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028882.1"
                     /translation="MHESFARRARSSPKSLLFPAAFAIGLAACSPHLVVNVEAPASAP
                     PETVALAKIVEIRELRHFEAAPKDPSTPSLAQAEMLDDPAIVARAIGRRRDAYGAAAG
                     DVLLPEGRTVIDLMRAVTTKALESDGYRVVDQSSPQYAQAAPLAVDIEQFWEWNGPSG
                     LGVQMEFKGIVTMRSAELIGTEPAIASGYARAYGAVLLTNNDRLDLFQRGMNDLAQNM
                     SAKNQGACGGENFGQLSSRASRERMRRTRCGIFLIDNTPRFAVS"
     gene            complement(1526..2305)
                     /locus_tag="VGP50_15730"
     CDS             complement(1526..2305)
                     /locus_tag="VGP50_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8028883.1"
                     /translation="MDFGIAGKKALVCAASKGLGKGCAMALAAEGAELVILARGKDAL
                     EATAAEIRAQTGAKVATVACDITTPEGRKVALAACPSPDILVNNAGGPPPGDFREWDR
                     DVWIKALDANMLTPIELIRATVDAMIARRFGRIVNITSSSVKAPIEVLGLSNGARSGL
                     TGFVAGIARKTVRHNVTINNLLPGAMDTDRLRANLALAAKNRNIALEQARAERIAAIP
                     AGRFGTIEDFGTACAFLCSQQASYITGQNWLVDGGAYPGTF"
     gene            2501..4201
                     /locus_tag="VGP50_15735"
     CDS             2501..4201
                     /locus_tag="VGP50_15735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEV8028884.1"
                     /translation="MTKRQVIAPALIATLAAPGLALATEPASLDITTAPVEVTIYDSQ
                     ATVLRQGEVEIPAGESIVVLHGLPAEIVEDSVTVKGTSAGKVVIGSIDTRAQLVAEAV
                     NRKRAELDAAIREIDDKIGLVDVRLAAFDAEKDFIAALSRLPQTPEQAPAPSWSNSET
                     WKSAWNTVHDGVLDAETNINTARHDRRQLEEERARLAARADGLGADAKRMLEIAIPVT
                     SDKPARLGLSVSYQVPHAAWRPIYDLRLDTTSGKVTVLQQATIMQSTGEDWSNVALKL
                     STAKPARGIAPPTLEPLRVALADLAQYRSRTRALAGKDDSDGLQRLILRAPAASTAAP
                     APAEAKTEAEPEPVPIAEAETIAAGLSVEFAVPGVVSVPSDPSQHKVRIGENSWDATF
                     FARTVPRVDSNAYLHARFKNGTAAAFLPGVAAAYVDGVYVGRENLPLARPGEEITAAF
                     GPDDRIRVKFEPQVEKTSVETAIIGKNKQVVERSFLTTVKSFHTRPLVVTVLDRIPVS
                     GDETLKVEVTTTPEPAAKNVDDKPGLIAWSQEYKPGDEAQFSLRYLLTAPQEKVITGF
                     "
     gene            complement(4198..5061)
                     /locus_tag="VGP50_15740"
     CDS             complement(4198..5061)
                     /locus_tag="VGP50_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV8028885.1"
                     /translation="MAATAALCLALAVSARAADDAAVKRGEVLFKAADCTGCHTDVKG
                     GGKPLAGGRPLDTPFGRFYSPNITPDKKNGIGGWSAAQFHDALRKGVDDDGDYLFPVL
                     PYTSFTGMSDQDIADLYAYMQTLPAVAQPDKPNEVSFPFSWRFLQLFWRTLFFTEGPL
                     QPVPGQSAEWNRGRYLAEAVAHCGECHTPRNFLGGLKRSQAYSGNPQGPDNLKVPNIT
                     PDDETGTGKWSLDDIATVLKTGQTPDFDFVGAGMSDVVKGTAALSDADRQAIAVYVKS
                     LPPIRRPKPAS"
     gene            complement(5100..5576)
                     /locus_tag="VGP50_15745"
     CDS             complement(5100..5576)
                     /locus_tag="VGP50_15745"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV8028886.1"
                     /translation="MRHLARVGIVTAALAAVIGAGAISGFAEDKEAVIKERRDAMKRQ
                     GDDLKAISEYAKGDGDQATATAKINDLLTLNPKIVDLFVPGTGMDAFPGKTGAKPEIW
                     KEWDKFKTLPAGLKTEEEKLAAAIKSGDKGAVGAQLAETGKNGCGGCHTPYREKLS"
     gene            complement(5685..5963)
                     /locus_tag="VGP50_15750"
     CDS             complement(5685..5963)
                     /locus_tag="VGP50_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028887.1"
                     /translation="MASHLCPVCGFDQLTKPAYDHRGFGSLEVCASCGFQFNISDTRH
                     GWSFEDWRKKWVSDGMLWSSKTVRPPRQWNPQNQIGRLQTGDPDLPHR"
     gene            complement(5974..6375)
                     /locus_tag="VGP50_15755"
     CDS             complement(5974..6375)
                     /locus_tag="VGP50_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HEV8028888.1"
                     /translation="MSSYPEERDYFDFDAEPPAGSDDVERPVADEGDAELQVKVIEAL
                     KTVRDPEIPVNLVDLGLIYELIVNKDGVVYVEMTLTTPACPVAGALPGQVRDAIASVP
                     GVQDARVKLIWSPPWTKDRMSEEAKLELGLL"
     gene            complement(6383..6847)
                     /locus_tag="VGP50_15760"
     CDS             complement(6383..6847)
                     /locus_tag="VGP50_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV8028889.1"
                     /translation="MDLRDLYQDIILDHGQHPRNFRKQAEPSHFANGHNPLCGDRVTV
                     YVTLDGERIKDVSFEGRGCAISQASASLMTEILKDKTLGEASTLFKGFHAKVTGAAAA
                     ATPEALADDMERLEPLTGVKAYPARVKCATLAWHAFEAALKSGAVGATIKTE"
     gene            complement(6850..8106)
                     /locus_tag="VGP50_15765"
     CDS             complement(6850..8106)
                     /locus_tag="VGP50_15765"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004271479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV8028890.1"
                     /translation="MTLVTTLRSDARGALDPRAIRRDFPIFERNPGLVFLDSGASAQK
                     PAAVIDGVADYYRSDYANVHRGVYRLSARSTELYEGAREKVRRFLNAADASEIVFVRN
                     ATEGINLVAQSWGATFLEPGDEVLITVMEHHANIVPWQLLRDRVGLKLVVAPIDATGG
                     LDMAAFEKLLSPRTKLVAVTQLSNVTGAILPVESIVRLAHARGAKVLLDGCQAAPRLP
                     VDVQALDCDFYVFSGHKTYGPTGIGVLYGKKAVLDTMPPWQGGGDMILEVTFEKTTFQ
                     EPPHRFEAGTPDISGAIGLGIALDYLASLGRDAVFEHEAALTGYGVDRLSTMPGVHLL
                     GAGQQRLAILSVHVDGIHPHDLATVLDQHKVAIRAGHHCAQPFMDRLGLAATARASLG
                     VYNDESDVDAFVDALKAAQRMFKVAP"
     gene            complement(8103..9416)
                     /gene="sufD"
                     /locus_tag="VGP50_15770"
     CDS             complement(8103..9416)
                     /gene="sufD"
                     /locus_tag="VGP50_15770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV8028891.1"
                     /translation="MTIVKAETVPYIEAFRAQAQAGEPDWLTAGREAALARFGSLGFP
                     TRRQEAWRFTDLRPLQRQAFPPAAGGATVSPDALRRYRLDPAAHSLVLVNGRFAPELS
                     RTGALPAGAWLASTRRTLEERPHLLEATIAETDIAGAQPFAALNAAFFADGVVLALDA
                     GVVLEEPIQIIHLASAAEPRSFHLRSVIALGAGSRATIIESFIGDGAAWTNAVTAVTI
                     GEGAALEHAVLQDESRAAIHFSLLRGTLAAGAAYESFALTLGARLSRRDAQVTIAGPD
                     ARCTLNGAYLLRGDQEATNATFVDHAAPGGTTRELWKGVADGRAHGAFLGRIAVRPDA
                     QKTDASQVNRNLLLSPRASIDTKPELEILADDVKCSHGATVGDLDADALFYLRARGIA
                     EAEARRMLIDAFAAEAIATVANPALRDHLLTELQRWLGAEEAVPS"
     gene            complement(9535..10287)
                     /gene="sufC"
                     /locus_tag="VGP50_15775"
     CDS             complement(9535..10287)
                     /gene="sufC"
                     /locus_tag="VGP50_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV8028892.1"
                     /translation="MLEITNLHARIADGREILRGIDLSVAAGEVHAIMGPNGSGKSTL
                     ASVLAGRDGYDVTEGSVTYQGRDLLALSPDERARQGVFLAFQYPVEIPGVNNMYFLRA
                     ALNAVRRARGQPDLDAGQFLKAVRAKLKVLEIDDDLLQRAVNVGFSGGEKKRNEIFQM
                     AMLEPKLAILDETDSGLDIDALRLVAKGINTLRAPDRAMLVITHYQRLLDHVAPDRVH
                     VLSEGRIVMSGDKSLATELEKRGYAMLEDAPA"
     gene            complement(10326..11780)
                     /gene="sufB"
                     /locus_tag="VGP50_15780"
     CDS             complement(10326..11780)
                     /gene="sufB"
                     /locus_tag="VGP50_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV8028893.1"
                     /translation="MNSSVTAQTDVRSLTGDGYKYGFVTDIESESAPPGLNEDIVRFI
                     SAKKEEPLWLLEWRLEAYRHWRKMEAPEWAKLRIAPIDYEGATYYSAPKRQNAPKSLD
                     EVDPELLRTYEKLGIPLSERAALAGVAVDAVFDSVSVATTFKKQLGALGIIFCSFGEA
                     VREHGDLVRKYLGSVVPPGDNFFAALNSAVFSDGSFVYVPPGVRCPMELSTYFRINAA
                     KTGQFERTLIVADKGSHVSYLEGCTAPQRDENQLHAAVVELVALDDAQIKYSTVQNWY
                     PGDAEGKGGIYNFVTKRGACRGVNAKISWTQVETGSAITWKYPSCILEGDNSVGEFYS
                     VAITNNLQQADTGTKMIHLGRNTRSTIISKGISAGRGQNTYRGLVRIQPKAANARNYT
                     QCDSLLIGHRCGAHTIPYIEVRNPTARVEHEATTSKISDDQMFYCRQRGLSPEDALSL
                     IVNGFCKEVLKELPMEFAVEAQKLLAVSLEGSVG"
     gene            complement(11777..12235)
                     /locus_tag="VGP50_15785"
     CDS             complement(11777..12235)
                     /locus_tag="VGP50_15785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HEV8028894.1"
                     /translation="MIILSKLADYGVIVATHLAAEPGRQMTAATLAAEARLPRATVAK
                     VLKALAHAGLVSGARGAAGGYRLARPATAISLAEVVSAIDGAIGLTQCTNHGPDCRRS
                     SFCPTKSAWQRINGAVGAALESVMLAEMLPPPPSFAAAPALAPSPDRLAP"
     gene            complement(12487..12888)
                     /locus_tag="VGP50_15790"
     CDS             complement(12487..12888)
                     /locus_tag="VGP50_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028895.1"
                     /translation="MNNRILLIDDDLIVRQTIVNVLTDAGYEVASAGDGLSGMRAYRH
                     SRPDLVITDIIMPDQEGIQTLMEIHKVDPTAKVIAISGGGRTSNLRILDVARTMGASD
                     TLAKPFDLEELLTVVRRWLPLDTDPPSNNQN"
     gene            complement(12885..>14835)
                     /locus_tag="VGP50_15795"
     CDS             complement(12885..>14835)
                     /locus_tag="VGP50_15795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8028896.1"
                     /translation="RESEAQLRRSREHLARAQRTADIGSWEIDLCSRSLEWSDELFRV
                     LGLSREQVAPGFDHMIARFCPEDRERTRGWLDLACAGAIVPASEGRLLLPDGRERWVR
                     MDAAALRDPAGEIETIVGTVEMITQRKLVELDLRHSQQHLARAQRIARVGSMEIDYRT
                     GKWIWTDELYRLLGIEKTVPPSAELYCSLLPEAFGERFVASVEATRHGLLQQRRETTL
                     IRPDGQVRTVEVDVDGLADQTGEVISGMATFHDITEFKRVEAELRDNEAQLRRSQEHL
                     KTAQRVAAIGSFEHRIGTSVLDWSEETYRIFGIELGTPITVEAQEALVLEEDREGLRN
                     TYRLAEEGQPVPAFEYRIRRSDGVVRTLHRECEILRDAAGAPTGIIGVCRDVTELRAE
                     QHRNAELEVQLHHAQRMEALGTLAGGIAHDLNNTLLPVMVLADALRRKLPAGSPDAER
                     LDLIRQAGDRARSLVRQILAFSRREDPNKTVLDLAALIRQSMGLVRAGVPTTVRIEEQ
                     IDRVAPVLGDAGQLHQVLLNLIGNAIDAIGDSIGTITLALNNEAQTVAGPGAPPGPCV
                     HFSVADTGCGMDDATLKRIFEPFFTTKAVGQGTGLGLSVVHGIVTSHGGRIEATSRRG
                     EGTRIDIFLPALGAEQTTDNKEIAA"
BASE COUNT         2401 a         4889 c         5040 g         2505 t
ORIGIN      
        1 ccgatgattg gcgacgtcgg caaagccgcc gactcgcggg tcgtcgaatc ccggaacgag
       61 cgcgagcgcg ggcggtgccg cgccgcaggc ggcgaagacc tcgcgcagca tggcggcggc
      121 tgcgtcgtag tccatgagcc catggccagc cggaaactcc gcgaagagat cgtcctcgaa
      181 gcggcagatg gcggcacgga aatcggtcgc gagatcgccg ccgcgaatcg cggcggcgat
      241 ggcttggccg aggccgcgct tggcgatgcg cgtaaatggc gtagcggcga cattcaccgt
      301 catgccgcca ttgtcgaccg gaaagggtta acgatgattt tatgcccggg cgaccgggca
      361 cttgcgccgc ctactgcgtc ggttggtaga agcggcggtc gagcagcacc tggagcgaga
      421 gggtcttgcc ctcatgctgc gcgccgacgc gcgacaagcg cgcatgcgga gcgacatcgc
      481 ctggatatcg cgccacggtg atgacctccc agacggcctt tgcgcgaccg accgggctaa
      541 aacgctgacc aatagtgatg ccgtaattgt cgtccggccc aagccggcgc cgccacatgc
      601 tcatgcgacc cttccacaca cacacacaca cacacacagc ccagccaaaa gcaggccgct
      661 ggcttgtcta tgaaaccgca aagcgaggcg tgttgtcgat aaggaaaatg ccgcagcgcg
      721 tacgtcgcat acgctcacga ctcgcgcggc tgctcaattg gccgaagttt tcgccgccgc
      781 aggcgccttg attcttcgca ctcatgttct gggcgagatc attcatgccg cgttgaaaga
      841 ggtcgaggcg atcgttgttc gtcaggagga cggcgccgta ggcgcgcgca tagccgctgg
      901 cgatcgcggg ctcggtgccg atgagctcgg cgctccgcat cgtgacgatc cccttgaact
      961 ccatctggac gccgagtccg ctcgggccgt tccactccca gaattgctcg atgtcgaccg
     1021 cgagcggcgc cgcctgcgca tattgcggcg agctctgatc gacgacccga tatccatcgc
     1081 tctcgagcgc cttggtcgtg acggcgcgca tgagatcgat cacggtccgg ccctcgggca
     1141 gcaggacatc gcccgcggcg gcaccatagg catcccgccg tcgtccgatg gcgcgcgcga
     1201 caatcgcggg gtcgtccagc atctcggcct gcgccagcga cggcgtggac ggatccttgg
     1261 gcgcggcctc gaaatggcgc aactcgcgga tttcaacgat cttcgcaagt gccaccgtct
     1321 ccggcggcgc gctggcgggc gcctcaacat tcaccacgag atgcgggctg caggcggcga
     1381 ggccgatggc aaaagcggcg ggaaagagca gcgacttcgg cgaggatcgg gcgcgtcgag
     1441 cgaaagattc gtgcatcggg caaaacctag cgtagagccg atggcgagac tactatcgtc
     1501 tgccgtgcac cgccagcgaa ttccctcaga acgttccggg ataggcgccg ccgtcgacga
     1561 gccagttctg gccggtgata tagctggcct gctggctgca caggaaggcg caggcggtgc
     1621 cgaaatcctc gatcgtgccg aatcgcccgg cgggaatggc cgcgatgcgc tcggcgcgcg
     1681 cctgctccag cgcgatgttc cggttcttcg ccgcgagcgc gaggttggcg cgcagccggt
     1741 cggtatccat cgcacccggc agcaggttgt tgatggtgac gttgtgccgc accgtcttgc
     1801 gggcgattcc ggcaacgaag ccagtgaggc cggagcgggc gccgttggac aggcccagga
     1861 cctcgatcgg cgccttcacc gagctcgagg tgatgttgac gatgcggccg aatcggcggg
     1921 cgatcatggc gtcgacggtg gcgcggatca gctcgatcgg cgtcagcatg ttggcgtcga
     1981 gcgccttgat ccagacgtcg cggtcccatt cgcggaaatc gccgggcggc ggcccgccgg
     2041 cattgttgac gaggatgtcg gggctgggac aagcggcgag cgcaaccttg cggccctcgg
     2101 gcgtggtgat gtcgcaagcg accgtcgcca ccttggcgcc ggtctgcgcg cgaatctccg
     2161 ccgccgtcgc ctcgagcgcg tctttcccac gcgccaggat cacgagctcg gcgccctcgg
     2221 cggccagcgc catggcgcag cccttgccca gccctttgct ggcggcgcag accagcgcct
     2281 tcttgccggc gatgccgaaa tccatggacc cctcccccgc ctgttccgcc tcatgataag
     2341 gctcggcgcc gacccgtcca gcgccgtcgc gcgaccaagg aggagcaggc cgccgatttg
     2401 ggaacgaagg aaagccgcgg cccgagcatg aaagtgccgc attttcgtcc aactcgcggt
     2461 tgaccatgcc tcctctcgac gaagcggagg acatcgcatc atgacgaaac gccaggtcat
     2521 cgcaccggcc ctgatcgcga cgctggcggc gccgggcctc gccttggcca ccgagcccgc
     2581 gtcgctcgac atcacgaccg cgccggtcga ggtgaccatc tacgacagcc aggcaacggt
     2641 gctgcgccaa ggcgaggtcg agatccccgc cggcgagagc atcgtcgtcc tgcatggcct
     2701 gcccgccgag atcgtcgagg acagcgtcac ggtgaagggc acatctgccg gcaaggtggt
     2761 catcggctcg atcgatacgc gagcccagct cgtcgccgag gccgtcaacc ggaagcgcgc
     2821 cgagctcgat gccgcgatcc gcgagatcga cgacaagatc ggcctcgtcg atgtgcgcct
     2881 cgccgccttc gatgccgaga aggacttcat cgcggcgctg tcgcggctgc cgcagacgcc
     2941 ggaacaagcg cccgcgccct cgtggagcaa cagcgagacg tggaaaagcg cgtggaacac
     3001 cgtgcatgac ggcgttctcg atgcggagac gaacatcaac accgcgcgac acgaccgccg
     3061 gcagctcgaa gaagagcgcg cgcggctcgc ggcgcgtgcc gatggcctcg gtgcggatgc
     3121 gaaacgcatg ctggagatcg ccatccccgt gacgtcggac aagccagcgc ggctcgggct
     3181 ctccgtcagc tatcaggtgc cgcacgcggc gtggcgtccg atctacgacc ttcgcctcga
     3241 caccaccagc ggcaaggtga cggtgctgca gcaagcaacg atcatgcagt cgacgggcga
     3301 ggattggtcg aacgtcgcgc tgaagctctc gaccgccaag ccggcacgcg gcatcgcgcc
     3361 gccgacgctc gagccgctgc gcgtcgccct cgccgacctc gcgcaatatc gctcgcgcac
     3421 ccgggcgctc gccggcaagg acgacagcga cggcctccag cgcctgattt tgcgcgcccc
     3481 cgccgcgtca accgcggctc cggcccccgc cgaggccaaa accgaggccg aacccgagcc
     3541 cgtgccgatc gcggaggccg agacgatcgc cgcgggcctc tctgtcgagt tcgccgtacc
     3601 gggcgtcgtg tcggtgccca gcgatccgag ccagcacaag gtgcggatcg gcgagaatag
     3661 ctgggacgcg acgttcttcg cgcgcacggt gccgcgcgtc gacagcaacg cctatcttca
     3721 tgcgcgtttc aagaacggca ccgcggcggc gttcctgccc ggagtcgcgg ccgcttatgt
     3781 cgacggcgtc tatgtcggcc gcgagaatct gcctttggcg cgtcccggcg aggagatcac
     3841 ggcggccttc ggccccgatg accgcatccg cgtgaaattc gagccgcaag tggaaaaaac
     3901 ctcggtcgag acggcgatca tcggcaagaa caagcaagtc gtcgagcgca gcttcctgac
     3961 cacggtcaag agcttccaca cgcggcccct cgtcgtgacg gtgctcgacc ggatcccggt
     4021 ctccggcgac gagacgctca aagtcgaggt cacgacgacg ccggagcccg ccgcgaaaaa
     4081 tgtcgacgac aaacccgggc tcatcgcctg gtcgcaagag tacaagcccg gcgacgaagc
     4141 gcagttcagc ctgcgctatc tgctgacggc gccgcaggag aaggtgatca ccgggtttta
     4201 gctcgcgggc ttgggccggc ggatcggcgg gagcgacttg acatagacgg cgatggcctg
     4261 gcggtcggcg tcgctgagcg cggcggtgcc tttgacgacg tcggacatgc cggcgccgac
     4321 gaaatcgaaa tcgggggtct gcccggtctt caagacggtg gcgatgtcgt cgagactcca
     4381 tttgccggtg cccgtctcgt catcgggcgt gatgttcggc accttcagat tatccggacc
     4441 ttgcgggttg ccggaatagg cctggctgcg cttcaggccg cccaggaagt tgcgcggcgt
     4501 gtggcactcg ccgcaatggg cgacggcctc ggcgaggtag cgtccgcgat tccattccgc
     4561 gctctgaccc ggcaccggct gcagcggccc ctccgtgaaa aagagcgtgc gccagaagag
     4621 ctggagaaaa cgccagctga agggaaagct cacttcattg ggcttgtcgg gctgcgccac
     4681 ggccggaagg gtctgcatat aggcgtagag atcggcgatg tcctggtccg acatgccggt
     4741 gaaagacgta tagggcaaca ccgggaagag atagtcgcca tcgtcatcga cgcctttgcg
     4801 caaggcgtcg tggaactggg ccgcgctcca gccgccgatg ccgttcttct tgtcgggtgt
     4861 gatgttgggg ctgtagaagc ggccgaacgg cgtgtcgagc ggccgtccgc cggcaagagg
     4921 cttcccgccg cccttcacgt cggtatggca gccggtgcaa tcggcggcct tgaacaggac
     4981 ctcgccgcgc ttgacggcgg cgtcatcggc cgcgcgcgcc gacaccgcca gcgccaagca
     5041 cagagccgcg gtcgcggcca cagcccagcg cttgaccgcg gctctcctcc gagtggtggt
     5101 tacgacagct tctcgcgata aggcgtgtgg cacccaccgc agccattctt gccggtctcg
     5161 gcgagctgcg cgccgacggc acccttgtcg ccgctcttga tcgcggcagc gagcttttcc
     5221 tcttcggtct tcaggcccgc cggcagcgtc ttgaacttgt cccattcctt ccagatctcc
     5281 ggcttggcgc cggtcttccc gggaaacgca tccatgccgg tgcccggcac gaacaaatcg
     5341 acgatcttcg ggttgagcgt cagcaagtcg ttgatcttcg ccgtcgccgt cgcctggtcg
     5401 ccgtcgccct tggcgtattc cgagatcgcc ttcagatcat cgccctggcg cttcatggcg
     5461 tcgcggcgct ccttgatgac cgcttccttg tcctcggcga agccgctgat cgcgcccgcg
     5521 ccgatcaccg ccgccagcgc tgccgtcaca attcctaccc gtgccaagtg ccgcattgat
     5581 tgctcccccg cttgaaatga cgtgtcgccg ctctcttccg cgcgacgccg gtgatgatac
     5641 ggacggcgac ctgccccatc cttcacgatt ccgtgatcga gcgatcagcg gtgcggcagg
     5701 tccgggtcgc cggtctgcaa gcggccgatt tggttctgtg gattccactg gcggggcggg
     5761 cgcaccgttt tcgacgacca cagcatccca tccgacaccc acttttttcg ccaatcttca
     5821 aagctccacc cgtgccgcgt gtcggagatg ttgaactgaa acccgcacga ggcacacacc
     5881 tcgagcgagc cgaaccctct atggtcatac gcaggcttgg tcaactgatc gaagccgcac
     5941 accggacaca aatggctcgc catctatttc ccgtcagagc agcccgagct cgagcttcgc
     6001 ttcctcgctc atgcggtcct tggtccaggg cggcgaccag atcagcttga cgcgtgcgtc
     6061 ctggacgccg ggcacggaag cgatcgcgtc ccggacctgg cccgggagcg cgcccgccac
     6121 ggggcaggcg ggcgtggtga gcgtcatctc gacatagacg acgccgtcct tgttgacgat
     6181 gagctcgtag atgaggccga gatcgaccag gttcacgggg atctcgggat cgcgcacggt
     6241 cttcagggcc tcgatcacct tcacctggag ctcggcgtcg ccttcgtcgg ccacgggacg
     6301 ctcgacgtcg tcgctcccgg ccggcggctc ggcatcgaag tcgaagtaat cgcgctcttc
     6361 gggataggac gacaagggaa cactactccg tcttgatggt ggcgccgacc gcgccgctct
     6421 tgagcgcggc ctcgaacgcg tgccacgcca gcgtcgcgca tttgacgcgc gcgggatagg
     6481 ccttgacgcc agtcagaggc tcgagccgct ccatatcgtc ggcgagcgct tcgggcgttg
     6541 ccgcggcggc agcgcccgtg accttggcgt gaaagccttt gaacagcgtg ctggcctcgc
     6601 cgagcgtctt gtccttcagg atctcggtca tcagcgacgc cgaggcctgc gagatggcgc
     6661 agccgcggcc ctcgaagctc acatccttga tacgctcgcc gtcgagggtg acatagaccg
     6721 tgacgcggtc gccgcagagc gggttgtggc cattggcgaa gtggctcggc tccgcctgct
     6781 tgcggaagtt ccgcggatgc tgcccgtggt cgaggatgat gtcctgatag aggtcgcgca
     6841 gatccatggt cacggcgcca ccttgaacat gcgttgcgcg gccttgaggg cgtcgacgaa
     6901 agcgtcgacg tcgctctcgt cgttgtagac gccgagcgaa gcgcgggcgg ttgcggcgag
     6961 gccgaggcgg tccatgaagg gctgcgcgca atgatggccg gcacggatcg ccaccttgtg
     7021 ctggtcgagg accgtggcga ggtcgtgcgg gtggattcca tcgacatgca ccgagagaat
     7081 ggcgaggcgc tgctgtcccg cgcccaggag atgcacgcct ggcatggtcg aaaggcggtc
     7141 gacgccatag ccggtgagcg ccgcctcatg ctcgaacacg gcgtcgcggc cgaggctcgc
     7201 gagataatcg agggcgatgc cgagcccgat ggcgcccgag atgtcgggcg tgccggcctc
     7261 gaagcgatgg ggcggctcct ggaaggtcgt cttctcgaaa gtgacctcga ggatcatgtc
     7321 gccgccgccc tgccagggcg gcatggtatc gagcaccgcc ttcttgccgt agagcacgcc
     7381 gatgccggtc gggccatagg tcttgtggcc ggagaagacg tagaagtcgc agtcgagcgc
     7441 ctgcacgtcg acgggcagcc ggggcgccgc ctggcagccg tcgagcagaa ccttggcgcc
     7501 ccgcgcatga gcgagacgga cgatgctctc gaccggcagg atcgccccgg tgacgttcga
     7561 cagctgagtc acggcgacga gtttcgtgcg cggcgacagc agcttctcga atgccgccat
     7621 gtcgaggccg cccgtcgcat cgatcggcgc caccacgagc ttcaggccga cgcggtcgcg
     7681 caggagctgc cagggcacga tgttggcgtg atgctccatc acggtgatga gcacctcgtc
     7741 accaggctcg aggaaggtcg cgccccagct ctgggcgacg aggttgatgc cttcggtggc
     7801 gttgcggacg aagacgatct cgcttgcgtc ggcggcgttg aggaagcggc gcaccttctc
     7861 gcgggcacct tcgtagagct cggtcgagcg cgcgctcagc cgatagacgc cgcgatgcac
     7921 gttggcatag tcgctgcggt agtaatcggc gacgccgtcg atgacggcgg caggcttctg
     7981 cgctgaggcg ccgctgtcga ggaagacgag gccgggattg cgctcgaaga tcgggaagtc
     8041 gcggcggatg gctcgcggat cgagcgcgcc ccgggcatcc gagcgtaaag tcgtgacgag
     8101 cgtcatgacg gaaccgcttc ctccgcgcca agccagcgtt gcagctccgt cagcagatgg
     8161 tcgcgaaggg ccgggttcgc aacggtggcg atggcctcgg cggcgaacgc gtcgatcagc
     8221 atgcgccgcg cctcggcctc ggcgatgccg cgcgcgcgca gatagaagag cgcgtccgca
     8281 tcgaggtcgc cgacggtcgc gccatggctg catttcacgt cgtcggcgag gatctcgagc
     8341 tcgggcttcg tgtcgatgct ggcgcgcggg ctcagcagga ggttgcggtt cacctggctg
     8401 gcgtcggtct tctgcgcgtc ggggcgcacg gcgatgcggc cgaggaacgc gccatgggcg
     8461 cggccgtcgg cgacgccttt ccacagctcg cgcgtggtgc cgcccggcgc cgcgtggtcg
     8521 acgaaagtgg cgttggtcgc ctcctgatcg ccgcgcagca gataggcgcc gttgagcgtg
     8581 cagcgtgcgt cggggccggc gatcgtcacc tgcgcatcgc ggcgcgacag ccgcgcgccc
     8641 agcgtcagcg cgaagctctc ataggcggcg ccggcggcca gtgtcccgcg cagcagcgag
     8701 aaatggatgg cagcgcggct ctcgtcctgg agcacggcat gctcgagtgc cgcgccttcg
     8761 ccgattgtca ccgccgtcac cgcgttcgtc caggctgccc cgtcgccgat gaagctctcg
     8821 atgatggtgg cgcggctgcc ggcaccgagc gcgatgacac tccgcagatg gaaggagcgc
     8881 ggctcggccg cgcttgccag atgaatgatc tgaatgggct cttcgagcac gacgcccgca
     8941 tcgagcgcga gcaccacgcc gtcggcgaag aaggcggcgt tgagcgcggc gaaaggctgg
     9001 gcccccgcta tgtcggtctc ggcgatcgtg gcttcgagga gatgcggccg ctcttcgagc
     9061 gtgcgccgcg tcgaagcgag ccaggcgccc gccggcagcg cgccggtgcg cgagagctca
     9121 ggggcgaaac ggccgttgac gagcacgaga gaatgcgcgg cgggatcgag acggtagcgg
     9181 cggagtgcgt ccggcgatac cgtcgcgcca cccgcggccg gtggaaacgc ctggcgctgc
     9241 agcggcctca ggtcggtaaa gcgccacgcc tcctggcggc gcgtcgggaa acccaagctg
     9301 ccgaaccgcg ccagggccgc ctcgcgcccg gccgtcaacc agtctggctc gccggcttgc
     9361 gcttgcgcgc ggaaggcctc gatatagggg acagtttcgg ctttgacgat ggtcatggcg
     9421 cgacccgtcg ctcgctcccc tcaccctccc agcgttgcgc gctgggtccc tccctctccc
     9481 acagagagga gggggaaagg caagcgagga atgcgaccgc ggacatcttc cacctcacgc
     9541 cggagcgtct tcgagcatgg cgtagccgcg cttctcgagc tcggttgcca ggctcttgtc
     9601 gccggacatg acgatgcggc cctcgctcag cacatggacg cggtcgggcg cgacgtgatc
     9661 gaggagccgc tggtaatgcg tgatcaccag catggcgcgg tcgggagcgc gcagcgtgtt
     9721 gatgcccttg gcgacgaggc gcagcgcgtc gatgtcgagg ccgctgtcgg tctcgtcgag
     9781 gatggcgagc ttgggctcga gcatggccat ctgaaagatc tcgttgcgct tcttctcgcc
     9841 gccggagaag ccgacattga cagcgcgctg caggagatcg tcgtcgatct cgagaacctt
     9901 gagcttggcg cgcacggcct tcaggaactg cccggcatcg aggtcgggct gaccccgcgc
     9961 gcggcgcacg gcgttgagcg cggcgcgcag gaaatacatg ttgttcacgc ccgggatctc
    10021 gaccgggtat tggaaggcga ggaagacgcc ctgacgcgcc cgctcgtcgg ggctgagcgc
    10081 caggaggtcg cggccctgat aggtgacgct gccctccgtc acgtcgtagc cgtcgcggcc
    10141 ggcgagaacg ctcgccagcg tgctcttgcc ggagccgttg ggccccatga tcgcatgcac
    10201 ctcgccggcg gcgacgctca gatcgatgcc gcggaggatc tcgcgcccat cggcgatgcg
    10261 ggcgtgcaga ttggttatct ccagcatcag tccgcttttc cctctgtctc ttttaattcc
    10321 ctggctcagc cgacgctgcc ttcgaggctc acggcgagca gcttctgcgc ctcgacggcg
    10381 aactccatgg ggagctcttt cagaacttcc ttgcagaagc cattgacgat gagcgacagc
    10441 gcatcctcgg gtgagaggcc gcgctgccgg caatagaaca tctgatcgtc gctgatcttc
    10501 gacgtcgtcg cctcgtgctc gacacgcgcg gtcggattgc gcacctcgat ataggggatg
    10561 gtatgcgcgc cgcagcgatg gccgatcagc agcgagtcgc attgcgtgta gttgcgggcg
    10621 ttcgcggcct tgggctgaat acgcacgagg ccgcgatagg tgttctggcc gcgcccggcc
    10681 gagatgccct tcgagatgat cgtcgagcgc gtgttgcggc ccagatggat catcttggtg
    10741 ccggtatccg cctgctgcag gttgttggtg atggcgaccg agtagaactc gcccacggaa
    10801 ttgtcgcctt cgaggatgca gctcggatat ttccaggtga tggccgagcc cgtctcgacc
    10861 tgggtccagg agatcttggc attgacgccg cggcaggcgc cgcgcttggt gacgaagttg
    10921 tagatgccgc ccttgccttc ggcgtcgcct gggtaccagt tctgcaccgt cgagtatttg
    10981 atctgggcat cgtccagtgc cacgagctcg accaccgcgg cgtggagctg gttctcgtcg
    11041 cgctgcggcg cggtgcagcc ctcgagatag ctcacatggc tgcccttgtc ggcgacgatc
    11101 agcgtgcgct cgaactgccc ggtcttcgcg gcattgatgc ggaaatacgt cgagagctcc
    11161 atcgggcagc gcacgcccgg cggcacatag acgaaagagc cgtcgctgaa cacggccgag
    11221 ttgagcgcgg cgaagaagtt gtcgcccggc ggcacgacgg agccgagata tttgcgcacg
    11281 aggtcgccat gctcgcgcac cgcctcgccg aaggagcaga agatgatgcc gagcgcgccc
    11341 agctgcttct tgaaggtggt ggcgacggag acgctgtcga agacggcatc gaccgcgacg
    11401 ccggcaagcg ccgcgcgctc gctcaaggga atacccagct tctcgtaagt gcgcagcagc
    11461 tcgggatcga cctcgtccaa ggatttcggg gcattctgac gcttgggcgc cgagtaatag
    11521 gtggcacctt cgtagtcgat cggcgcgatg cgcagcttcg cccattccgg cgcttccatc
    11581 ttgcgccagt ggcgatacgc ctcgaggcgc cattcgagca gccagagggg ctcctctttc
    11641 ttcgccgaga tgaagcggac gatgtcctcg ttgaggccgg gcggcgccga ttcgctttcg
    11701 atgtcggtga cgaagccgta tttgtagcca tcgccggtca gcgagcgcac atcggtctgt
    11761 gccgtgacgc tgctgttcat ggcgccagcc gatccggcga cggcgccaga gcgggagccg
    11821 cagcgaagct tggaggcggc gggagcatct ctgccagcat caccgactcg agggcggcgc
    11881 cgaccgcgcc gttgatgcgc tgccacgccg acttcgtcgg gcagaagctg ctgcgccggc
    11941 agtcgggccc gtgattcgtg cattgcgtga ggccgatggc gccgtcgatc gcggagacca
    12001 cctccgccag cgaaatcgcc gtcgcggggc gcgccagccg atagccgccg gcggcgccgc
    12061 gcgcgccgga caccagcccc gcatgggcca gcgccttcag cactttggca acggtggcgc
    12121 gcggcagccg cgcctcggcg gcaagcgtcg ccgcggtcat ctgccgcccg ggctccgccg
    12181 ccagatgcgt ggcgacgatg acgccgtagt cagcgagctt gctcaggatg atcatcgcga
    12241 gggcaatggc ttcaggggct taggtttaat tcggactgct ttagtgctag atcgtgtctt
    12301 acaccccggc gtcaaggccc cgcaagcgct atttttcatg cttccggcgg gaaaagcagg
    12361 aaagggtcgg gccagccatc gcgcgcagcc tgcccccggg ataactcgaa gttcactgtc
    12421 tgttctagat ttcggcccgg tgcttcgagc accggccaag gacgcgcccg cggcggcgct
    12481 tggcgatcaa ttctgattgt tcgacggagg atcggtgtcg aggggaagcc aacgccggac
    12541 gaccgtgagc agctcctcga gatcgaaagg ctttgccagg gtatcgctgg cgcccatggt
    12601 gcgcgccacg tcgagaatgc gcaggttgga ggtgcgcccg ccgccggaaa tagcgatcac
    12661 cttcgccgtc gggtcgacct tgtggatctc catcaaggtc tgaatcccct cctggtccgg
    12721 catgatgatg tcggtgatca cgaggtcggg gcggctgtgg cgataagccc gcatacccga
    12781 taggccgtcg ccggccgacg ccacctcgta gcccgcgtcg gtcaacacat tgacgatcgt
    12841 ctggcgcacg atgaggtcgt catcgatcag caggatacgg ttgttcacgc ggctatctcc
    12901 ttgttgtccg tggtctgttc ggcgccgagc gccggcagga atatgtcgat gcgggtaccc
    12961 tctccgcgcc ggctcgtcgc ctcgatgcgt ccgccatggc tcgtcacgat gccgtggacc
    13021 accgaaagtc cgagcccggt gccctggccg accgccttgg tcgtgaagaa cggctcgaag
    13081 atccgcttca gcgtcgcgtc gtccatgccg cagccggtgt cggcgaccga gaaatggacg
    13141 cagggtcccg gcggcgcgcc cggcccggcg accgtttgcg cttcgttgtt gagggccaag
    13201 gtgatcgtcc cgatgctgtc gccgatcgcg tcgatggcgt tgccgatcaa gttcaacagc
    13261 acctgatgca gctgcccggc gtcgccgaga accggcgcga cgcggtcgat ctgctcttcg
    13321 atccggaccg tcgtcggcac gccggcacgc accagaccca tgctctggcg gatgagcgcg
    13381 gcgaggtcga gcacggtctt gttgggatcc tcgcgccgac tgaaggcgag gatctggcgc
    13441 acgagactcc gcgcccggtc gcccgcctgg cggataaggt ccagccgctc ggcgtcgggg
    13501 ctgccggccg gcagcttgcg ccgcaaggca tcggccagga ccatcactgg cagcagtgta
    13561 ttgttgagat catgcgcgat tccgccggcg agcgtgccca gtgcctccat gcgctgcgca
    13621 tggtggagct gcacttcgag ttcggcgttc cgatgctgct ccgcgcgcag ctcggtcacg
    13681 tcgcggcaca cgccaatgat gccggtcggc gcgcccgccg cgtcgcgtaa tatttcgcat
    13741 tcgcgatgga gcgtgcgcac cacaccgtcg gagcggcgga tacgatactc gaaggcgggc
    13801 accggctggc cttcctcggc caaccggtag gtgttcctga gcccctcgcg gtcttcctcg
    13861 aggacgagcg cttcctgtgc ctcaacggtt atgggcgtgc ccagctcaat gccgaagatg
    13921 cgataggttt cctccgacca atcgaggacc gaagtgccga tgcgatgctc gaagctgccg
    13981 atggcggcga cgcgctgggc ggtcttgaga tgctcctggc tgcgccggag ctgcgcttcg
    14041 ttgtcgcgaa gctcggcctc gacgcgcttg aattcggtga tgtcgtggaa cgtggccatg
    14101 ccgctgatca cctcgccggt ctggtcggcc aggccgtcga catcgacctc gacggtgcgc
    14161 acctggccat cgggccggat gagcgttgtt tcgcgccgct gctgcagcaa tccgtgccgg
    14221 gtcgcctcga cgctcgcgac gaaacgctcc ccgaacgcct ccggcagcag gctgcagtag
    14281 agctccgccg acggtggaac ggtcttttcg atgccgagca atcggtaaag ctcgtcagtc
    14341 catatccatt tgccggtccg gtagtcgatc tccatgctgc cgacgcgggc gatgcgctgt
    14401 gcccgcgcca ggtgctgctg gctgtggcgc agatcgagct cgacgagctt gcgctgggtg
    14461 atcatctcga ccgtgccgac gatggtctcg atctctcccg ccgggtcgcg tagcgccgcg
    14521 gcgtccatgc gcacccaacg ctccctgccg tcgggcaaaa ggagccggcc ctcgctggcc
    14581 gggacgatcg cgcccgcgca ggccaagtcg agccaacctc tcgtccgctc gcgatcttcc
    14641 gggcaaaagc gcgcgatcat atgatcgaat cccggcgcca cctgctcgcg cgaaaggccg
    14701 agcacgcgga acagttcgtc cgaccactcg agcgaacggc tgcacagatc gatttcccag
    14761 ctgccgatat cggcggtgcg ctgcgcccgg gcgagatgct cgcggctgcg gcgaagctgc
    14821 gcctcgctct cgcgc
//
LOCUS       DASYNS010000509         6145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5587392, whole genome shotgun sequence.
ACCESSION   DASYNS010000509 DASYNS010000000
VERSION     DASYNS010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5587392"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..590)
                     /locus_tag="VGP50_15800"
     CDS             complement(132..590)
                     /locus_tag="VGP50_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028897.1"
                     /translation="MSDEVTRRWQERIVRYRQLSTLFGKHGAANPLAAIVSDLEQLRQ
                     DQQLLFAKSTEQMSYTQVLVAEIDTVLARARGAGEVAGRLLSPSLTFSADDAREESLL
                     CCEEALATENLVGRRVLAARAAALALVAEQIDRRGSDHEPEASSAEGRRG"
     gene            850..3309
                     /locus_tag="VGP50_15805"
     CDS             850..3309
                     /locus_tag="VGP50_15805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like protein"
                     /protein_id="HEV8028898.1"
                     /translation="MREKELRIALVCFGGISLAVYIHGVSKEILKLVRASSALHEIRD
                     RAARARARFTDRAEAARDHDTEAVYFELLRDIGHKIDLRVIVDIIAGASAGGINGTML
                     ARSLAHDLPMDALRDLWLKEADVTELLAAEARPTRWSKLFMVPFIWAAGAMRWLGDVR
                     DVEVRQKLSLFVRSRWFKPPFDGTRMSQLMYDAVAASGAPASPTSSLLPAGQRLDLFV
                     TVTDHDGYRQLLETHDPPVIEEREHRHTLRFSCRRWPNGEIESDFDLADAPALAFAAR
                     ASSSFPGAFPPAQIVEMERMLAARGIAWPRQQQFLERNFAPYLRARLDPRAQCFIDGS
                     VLNDKPFHEAIQAIKGRAAYRQVDRRLVYVDADPERPPPPLQGVVPGFFTTLRHALSD
                     IPRNEPIADELAYVNAFNERVRRLKGIVEDARPQITRLVSGVVDLAADQALSPEQVRT
                     WRERVNAEVARDAGFAYEGYVRLKLAAVRALVAQLIVGMMEFPERSPVERTIAAIVDA
                     WARENRIFYEGGGGVPLTQERVVDAQTPRWVRFLLSFDIDFRKRRLYFLIQGQNRLYQ
                     MLDEDGGGTAAAVDRLKRAFYHSLDGLRRCESPSFFSAATRDVARALFGALPAGECWR
                     EEAPARRFAIANATAIDRLIGYMAAEINLDASTHEVDLLLAAADPAEWTQAARREVLV
                     NYLGFPFWDVLTLSVTNWRDIGEFDEILVDRISPRDARLLAAVGQARVPKGAGMAHFA
                     AFFSRGYREHDYLLGRLHGIDRLIDIVCNAAGIAPGKEGIDVLGLKRRAFAILLDAEE
                     KHLPRSAELIRSLRASLAAMA"
     gene            complement(3528..5024)
                     /locus_tag="VGP50_15810"
     CDS             complement(3528..5024)
                     /locus_tag="VGP50_15810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8028899.1"
                     /translation="MLFVNRALHRYPELGARRMYLALVVVVTISLYYTLYVAGGVAPL
                     LLAGLKLPFQAFVYILAAGNLAGAFASLFAGLADRFGRAQLVVWGLLIVGLLTLFVMP
                     NVTTGIEWGIAFGVISFVEGVILVATPALIRDFSPQVGRATAMGFWTIGPVLGSLTVS
                     MVTTLTLPIFQTWQSAYEICGIVGLVVFILAFLFLKELAPELRDQLMVSERDRALVEL
                     KAKGLDIAASLRNPWRQMLQVDVIASALGVSLLLLFYYTAVAFGLIYVVTVFSFSVEQ
                     ANELGNWAWAANAVALIAAGVLSDRLRIRKPFMIVGGVGGLVFTYLYLIQAGSHPSFV
                     TLTLISAAQSFFVGIAYVTWMASFTETVEARNPALTATGLAIWGWLLRLVVTACFAGL
                     PSVVRSVNPLITAPGVLAAYQQTVAQHVKPSPELLAALGEIKAAAAAAPGEWQTWYWI
                     CAAGIVVFIATVFLMRGRWSPSAARADEAAHDAEVARELARLRSQPAE"
     gene            5148..5534
                     /locus_tag="VGP50_15815"
     CDS             5148..5534
                     /locus_tag="VGP50_15815"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEV8028900.1"
                     /translation="MIRGYTDHSANERTFLAWVRTGIAVIAFGFVIERFNLFLLTLAS
                     AAAPEAARQLRVEGLPGPLGRYDGLALIAVGIALIVAAAARFVRTSRLLDDEAMHSAA
                     GVRAEVILSAALALIVAGFSAYLVLG"
     gene            complement(5669..>6145)
                     /locus_tag="VGP50_15820"
     CDS             complement(5669..>6145)
                     /locus_tag="VGP50_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007436308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8028901.1"
                     /translation="LPPSAELQWWYQFYFATERGEAGYDKYRHDFAKLIWQLASPKWN
                     FDAATFDRSAAAFANPDHVAISIHNYRWRLDLAKGEPKYDALERRLAAGPVIGVPTIT
                     LEGDANGAPHPDPASYAKKFSGKYAHRLIKGGVGHNLPQEAPQAFAEAVVEVAAEA"
BASE COUNT         1056 a         2044 c         2035 g         1010 t
ORIGIN      
        1 gagcgccatc ccggcggcta cacctgtcac ttcatccggc ccggctggaa acttccggtc
       61 cggcagggcg cgcgaaaggc gagttgattt tgcgatgggc gcgcgtggcg cgcccgtgcc
      121 acgacggtcg cctagccgcg gcgcccttcg gcagacgacg cttcgggctc atggtcgctg
      181 ccgcgacggt cgatttgctc cgccacaagg gcaagcgcgg ccgcgcgggc ggcgagcacg
      241 cgccgcccga cgaggttttc ggtcgccaga gcttcctcgc aacagagcag cgactcctcg
      301 cgggcgtcgt cggcgctgaa cgtcaaagag ggcgatagca gccggcccgc aacctcgcct
      361 gcgccccgtg cccgcgccag cacggtatcg atctccgcca ccagcacctg cgtgtaagac
      421 atctgctcgg tcgattttgc gaaaagcagc tgttggtctt gccgaagctg ctccaggtcg
      481 ctgacgatgg ccgcgagcgg attggcggcg ccgtgcttgc cgaacagtgt cgaaagctga
      541 cgataacgca caatacgttc ctgccagcgt cgcgtaactt catcgctcat tgtcacgaaa
      601 tcgtccccgt tccgaggcgc atctgggatg catgcagaga catgcatcca ggctaacaca
      661 accgcgccat gctaccaagg cggccaagag cacgttctgg cgagcgcccg accggcgccg
      721 ttctcgtgcc gcagcttcag ccagcggcca cgacggcatg atgcgagcct ccgaaaatgc
      781 taacctccgg cctgcgacag gaggggagga acgggggcgc ccgtgccggc gccctcgcgg
      841 atgaggggga tgcgcgagaa agaacttcgc atagcgcttg tctgcttcgg cggcatctcg
      901 ctcgccgtct atatccatgg cgtcagcaag gagatcctga agctggtgcg ggcctcgagc
      961 gcgctccacg agatccgcga ccgggcggcg cgggcgcgcg cccgcttcac cgaccgggcc
     1021 gaggcggcgc gcgaccacga caccgaagcg gtctatttcg agctgctgcg cgacatcggc
     1081 cacaagatcg atctgcgcgt catcgtcgat atcatcgccg gcgcctccgc aggaggcatc
     1141 aacggcacca tgctcgcgcg ttcgctcgct catgacctgc cgatggacgc gctgcgcgac
     1201 ctctggctca aggaggccga cgtcacggag ctgctggcgg cggaagcgcg cccgacccga
     1261 tggagcaagc tcttcatggt gcccttcatc tgggcggcgg gcgcgatgcg ctggctcggt
     1321 gacgtgcgcg acgtcgaagt ccgccagaaa ctgtcgctct tcgtccgctc gcgctggttc
     1381 aagccgcctt tcgacggcac gcgcatgtcg cagctcatgt atgacgccgt ggccgcttcc
     1441 ggcgcgccgg caagccccac gagttcgctc ctgccggcgg gccagcgact cgatctcttc
     1501 gtcaccgtca ccgaccatga cggctatcgc cagctgctcg agacccacga tccgccggtg
     1561 atcgaggagc gcgagcaccg ccatacgctc cggttcagct gccggcgctg gcccaatggc
     1621 gagatcgaga gcgatttcga cctggccgat gcgcccgcgc tcgcctttgc cgcccgcgcc
     1681 agctcgtcct ttcccggcgc cttcccgccg gcgcagatcg tcgagatgga gcggatgctg
     1741 gccgcccgcg gtatcgcctg gccgcggcag cagcaattcc tcgagcgcaa tttcgcgccc
     1801 tatctgcgcg cccgtctcga tccgcgcgcg caatgcttca tcgacggcag cgtgctcaac
     1861 gacaagccgt tccacgaagc gatccaggcg atcaagggcc gggcggccta ccggcaggtc
     1921 gaccggcggc tcgtctatgt cgatgccgac cccgagcggc cgccgccgcc gctccaaggc
     1981 gtggttcccg gcttcttcac gacgctccgg cacgcgctct ccgacattcc gcgcaacgag
     2041 ccgatcgccg acgagctcgc ctatgtcaac gccttcaacg agcgggtgcg gcggctcaag
     2101 ggcatcgtcg aggacgcgcg cccgcagatc acccgcctcg tatcgggcgt cgtcgatctc
     2161 gccgcggatc aggcgctgtc gccggagcag gtcaggacat ggcgcgagcg ggtgaacgcc
     2221 gaggtcgccc gcgacgccgg cttcgcctat gaaggctatg tgcggctgaa gctcgcggcg
     2281 gtgcgcgctc tcgttgcgca gctgatcgtc ggcatgatgg agttcccgga gcgctcgccg
     2341 gtcgagcgca ccatcgcggc gatcgtcgat gcttgggcac gcgagaacag gattttctat
     2401 gaaggcggcg gcggcgtccc gctgacgcag gagcgcgtcg tcgacgcgca gacgccgcgc
     2461 tgggtccggt tcctcctctc cttcgacatc gatttccgca agcgtcggct ctatttccta
     2521 atccaggggc agaaccggct ctaccagatg ctcgacgagg acggcggcgg caccgccgct
     2581 gcggtcgatc gcctgaagcg cgcgttctac cactccctcg acggcttgcg tcgttgcgag
     2641 tccccgagct tcttcagcgc ggcgacacgc gatgtggcac gggcgctgtt cggcgcgcta
     2701 ccggccggcg agtgctggcg cgaggaagcg ccggcgcgac gcttcgccat cgccaacgcc
     2761 acggcgatcg atcggctcat cgggtacatg gcggccgaga tcaatctcga cgccagcacc
     2821 catgaagtgg acctgctgct cgcggccgcg gatccggcgg aatggacgca agcggcgcgg
     2881 cgcgaggtgc tggtcaacta tctcggattc cccttctggg acgtgctgac gctatcggtg
     2941 acgaactggc gcgacatcgg cgagttcgac gagatcctgg tcgaccgcat cagcccgcgg
     3001 gacgcgcggc ttctcgccgc cgtcggccag gcgcgcgtgc ccaaaggcgc gggcatggcg
     3061 catttcgcgg cgttcttctc gcgcggctat cgcgagcatg actatctgct gggccggctg
     3121 cacggcatcg accggctgat cgacatcgtc tgcaatgccg cggggatcgc gcccgggaaa
     3181 gagggcatcg acgtgctcgg cctcaagcgc cgcgccttcg ccatcctcct cgatgccgag
     3241 gaaaagcacc tgccgcggag cgcggagctc atccgctcgc tccgcgcgtc cctcgcggcg
     3301 atggcgtaag gcgtggctct ccctcaaaga gcctgtgaat ttattcgcca aacaagttgg
     3361 atcactaggg tacgtccttc gacatggcgc ttcgcgccaa gctcaggatg aggatttttc
     3421 tcaatgccat caacaatata tcctcatcct gagcgggacc ctgagcttgg cgaaggggag
     3481 cagtcgaagg acgcacgatc gacctgcagc catgtgagga ctcgcgttca ctccgcgggc
     3541 tggctcctca gccgcgccaa ttcgcgcgcc acctctgcgt catgggcggc ttcgtcggcg
     3601 cgagcggcgg acggactcca acgtccgcgc atcaggaaga ccgtcgcgat aaagacgacg
     3661 atcccagccg cgcagatcca ataccaggtc tgccactcgc caggggccgc ggcagcggcg
     3721 gccttgatct cgccgagcgc cgcgagcagc tccgggctcg gtttcacatg ctgggcgacc
     3781 gtctgctgat aggcggcgag gaccccgggc gcggtgatca gcggattgac cgaacgcacg
     3841 acggagggca agccggcgaa acaggcggtg accaccaagc gaagcaacca gccccagatc
     3901 gccaggccgg tcgccgtcaa ggccgggttt cgcgcttcga cggtctcggt gaagctcgcc
     3961 atccaggtga cataggcaat gccaacgaag aaggactgcg cggcgctgat gagcgtgagc
     4021 gtgacgaagg acggatggct gcccgcctga atcagataga gataggtgaa gacgagtccg
     4081 ccgaccccgc ccacgatcat gaacggcttg cgtatgcgca gtcggtcgga tagcacgccg
     4141 gccgcgatga gcgccacggc gttggctgcc caggcccagt tgcccagctc attggcctgc
     4201 tcgacggaga aggaaaagac cgtcacgaca tagatcagcc cgaaggcgac ggcggtgtag
     4261 tagaacaaca gcaaaaggga aacgccgagg gcggaagcga tcacgtcgac ttgcagcatc
     4321 tgccgccagg gattgcgcag gctcgcggcg atgtcgaggc ccttcgcctt gagctcgacg
     4381 agcgcgcggt cgcgctcgct gaccatgagc tggtcgcgca gctccggcgc cagctccttc
     4441 aagaacagga aagcaagtat gaagacgacc aggccgacga tgccgcagat ctcataggcg
     4501 ctctgccaag tctggaaaat cggcagcgtc agggtcgtca ccatgctgac ggtcaggctg
     4561 ccgagcaccg ggccgatggt ccagaagccc atcgcggtgg cccggccgac ctggggcgag
     4621 aaatcgcgaa tgagcgcggg cgtcgccacc agaatgacgc cctcgacgaa ggagatcacg
     4681 ccgaaggcga tcccccactc gatgccggtg gtcacattgg gcatcacgaa caaggtcaag
     4741 aggccgacga tgagcagccc ccagaccacg agctgggcgc gtccgaagcg gtccgcgagg
     4801 ccggcgaaca gggaagcgaa ggccccggcc aggttgccgg ccgccaggat atagacgaag
     4861 gcttggaagg gcagcttgag ccccgccagc agcaatggtg ccacgccgcc ggccacatag
     4921 agggtgtagt agagcgatat cgtgaccacg acgacgagag cgagatacat gcgccgtgca
     4981 cccagttcgg gataccggtg caaggcgcgg ttgacgaaca acatgagaac ctccctgttt
     5041 cacgccggcc gatcgccggt catcgagcgt tgcagtttgc gatgttaatg aaattcgcat
     5101 acccctgcat ggttttccgg cggtcaaatg actgatagaa gtatctcatg atccgcggct
     5161 acaccgatca ttcggccaac gagcgaacct tcctcgcctg ggtgcgcacc ggcattgccg
     5221 tgatcgcgtt cggcttcgtc atcgagcggt tcaatctgtt cctgctgacc ttggccagtg
     5281 cggccgcgcc cgaggcggca cgtcagttgc gggtggaagg gctgccgggc ccgctcggcc
     5341 gttacgacgg actcgctctc atcgccgtcg gaatcgcgct gatcgtcgcc gccgccgcgc
     5401 gctttgtccg gacgagccgg ctcctcgacg acgaggcaat gcattcggcg gccggcgtcc
     5461 gcgccgaggt aattctgtcg gcagcgctgg cgttgatcgt cgccggcttc agcgcttatc
     5521 tcgtgcttgg ttgacgcgcc gcgagtgatt cggagggcgt gcagcggatt ttcttgcgag
     5581 cgcctctcac cctcccgccg ctgcgcggcg ggcccctccc tgtcccacgg tgcgggagag
     5641 ggctatagca tgccggccgc cggttcgatc aagcttccgc cgccacctcg accacggcct
     5701 cggcgaaggc ttgcggggct tcttgcggca gattgtgccc gacgccgccc ttgatcaggc
     5761 ggtgagcata cttgcccgag aatttcttgg cataggaagc ggggtccggg tgtggcgcgc
     5821 cattggcgtc gccttcgagg gtgatcgtgg gcacgccgat gaccgggccc gcggccagcc
     5881 ggcgctccag agcgtcgtat ttgggctcgc cttttgcgag gtcgagccgc cagcggtaat
     5941 tgtggatgga aatggcgaca tgatccggat tggcaaaggc cgcggcgctg cggtcgaacg
     6001 tggcagcgtc gaagttccac ttcggcgagg cgagctgcca gatgagcttc gcgaaatcgt
     6061 gccggtattt gtcatagccg gcctcgccgc gttccgtcgc gaaatagaat tggtaccacc
     6121 attggagctc ggcggatggc ggcaa
//
LOCUS       DASYNS010000510         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3093851, whole genome shotgun sequence.
ACCESSION   DASYNS010000510 DASYNS010000000
VERSION     DASYNS010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3093851"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VGP50_15825"
     CDS             <1..206
                     /locus_tag="VGP50_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028902.1"
                     /translation="PLKSVTVSAHDAAPALLSWAAEPPLEAPVQLLDLAAPSGHLAAL
                     ALLGERLDVVERDAAPLLAGPNI"
     gene            complement(196..1482)
                     /locus_tag="VGP50_15830"
     CDS             complement(196..1482)
                     /locus_tag="VGP50_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEV8028903.1"
                     /translation="MNAALIFALLLALMLTGMPISISLGLTVLTFVFTMAQVDTKMVA
                     VKLFTGIENFQIMAIPFFILAGSFLTHGGVARRMIAFTTSLVGHWAGGLALAGVVACA
                     LFAAISGSSVATVVAIGSIIMPAMVEHGFPRRFGAGVITTSGALGILVPPSIILVLYG
                     VSTSTSIGALFMAGVVPGLTLASALAAVTWFYAWRNGYPRLRRASWAARAAAFRDSVW
                     GLLLIGIVLGGIYSGLFTPTEAAAVAAVYAFVVAVFVYRDLPLSRVPKVLLDAANLSA
                     MLLYIITNAVLFSFLMTNENIPQAMAAWITGRHFSVWAFLLVVNLLLLAAGNVMEPSS
                     ILLIMAPILYPIVTKLGVDPVHFGILMAVNMEVGLCHPPVGLNLYVASGIAKMGISEL
                     TIAVLPWLLTMLVFLGVVTYWPGLSLWLPHLLGIRY"
     gene            complement(1479..2051)
                     /locus_tag="VGP50_15835"
     CDS             complement(1479..2051)
                     /locus_tag="VGP50_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEV8028904.1"
                     /translation="MLKLPLRVLDRLEEILIASLMAGATLITFAAVVHRYLSTIPAIQ
                     DYVTRIDMTWAQELCVYMFIWVAKFGAAYGVRTGIHVGVDVLVKRLPPRPRRGVIVFA
                     LLAGALFTGVIGTLGAQLVWDRAQTEDTSPDMEAPMWIVYLAIPCGSYLMCFRFLQVA
                     WRFAWTGQLPSHDPTHVEGIAPLPSDGPDG"
     gene            complement(2077..3078)
                     /locus_tag="VGP50_15840"
     CDS             complement(2077..3078)
                     /locus_tag="VGP50_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEV8028905.1"
                     /translation="MRPALVIAGLLLAIGLAPARAEEPIVIKFSHVVAPDTPKGKAAE
                     KFKALAEERTHGRVRVELYPNSQLYKDKEELEALQLGAVQMLAPSLSKFGPLGIKEFE
                     AFDLPYIFPSTEALRRVTDGPVGRDLLKKLEPKGILGLAYWDNGFSIITSNKPIRKLE
                     DLKGVKMRIQSSKVLEATYRHIGALPQIMAFSEVYQALQTGVVDAVETTPSNSYTQKF
                     YEVQKYLTVSDHTYIGYAVVVNKKFWEGLPADIRAALDSAMKDATVYCNEIAEKENEE
                     ALAKIKASGDVELIVLSDQEKAEWRKGLLPVHEEMAPRIGKDLIQAIYKETGTGATQ"
BASE COUNT          604 a         1033 c         1133 g          539 t
ORIGIN      
        1 ttccgctgaa gagcgtcacg gtgagcgccc atgacgctgc tccggcgctg ctctcctggg
       61 ccgccgagcc gccgctcgag gctccggtgc agctcctcga tctcgcggcg ccgtccgggc
      121 atctggccgc gcttgccttg ctcggcgagc gcctcgacgt cgtcgagcgc gatgccgcgc
      181 cgctgctggc gggccctaat atctgatccc caagaggtgc ggcagccaga gcgacaggcc
      241 cggccaatag gtgacgacgc cgaggaagac cagcatcgtg aggagccagg gcagcaccgc
      301 gatggtgagc tcgctgatgc ccatcttggc gatgccgctg gccacataga gattgaggcc
      361 gacgggcggg tggcagagcc ccacctccat gttcaccgcc atcaatatcc cgaaatgcac
      421 gggatcgacg ccgagcttgg tgacgatcgg atagaggatg ggcgccatga tcagcaggat
      481 cgacgacggc tccatcacat tgccggcggc gagaaggagc aggttcacga ccagcaggaa
      541 ggcccagacg ctgaaatgcc ggccggtgat ccacgccgcc atggcctgcg ggatgttctc
      601 attggtcatg aggaaggaga acagcacggc gttggtgatg atgtagagca gcatggcgct
      661 cagattggcc gcatcgagca ggactttggg cacgcggctg agcggcaggt cgcgatagac
      721 gaagacggcg acgacgaagg catagacagc ggcgaccgcc gcggcctcgg tcggcgtgaa
      781 gaggccgcta tagatgccac ccagcacgat gccgatgagc agcagccccc agacgctgtc
      841 gcggaaagcg gcggcgcgcg ccgcccagct ggcgcggcgc aagcgcgggt agccgttgcg
      901 ccaggcatag aaccaggtga ccgccgcgag cgcgctcgcc agcgtcaggc cgggaacgac
      961 gcccgccatg aagagcgcgc cgatcgaggt gctggtcgag acgccataga gcaccaggat
     1021 gatcgagggc ggcacgagaa tgccgagtgc acccgaggtt gtgatcacgc cggcgccgaa
     1081 gcggcgcgga aagccatgct cgaccatcgc cggcatgatg atggagccga tcgctacgac
     1141 cgtcgcgacg ctcgacccgg agatcgccgc gaagagcgcg caggcgacga cgccggccag
     1201 cgcgagaccc cccgcccagt gaccgaccag cgaggtggtg aacgcgatca tgcgccgggc
     1261 cacaccgcca tgggtgagga agctgccggc gaggatgaag aacgggatcg ccatgatctg
     1321 gaagttctcg atgccggtga agagcttcac cgccaccatc ttcgtgtcca cctgggccat
     1381 ggtgaagacg aaggtgagga cggtgaggcc gagcgagatc gagatcggca tgccggtgag
     1441 catgagcgcc agcagcagag cgaagatcag cgcggcattc atccgtccgg tccgtccgaa
     1501 ggcagcggcg cgatcccctc gacatgtgtc gggtcatggg acgggagctg gccggtccag
     1561 gcgaaccgcc acgccacctg gaggaagcgg aagcacatga ggtaggagcc gcacgggatc
     1621 gccagataga cgatccacat cggtgcttcc atgtcgggtg acgtgtcctc ggtctgcgcc
     1681 cggtcccaga cgagttgcgc gccgagcgtg ccgatgacgc cggtgaagag cgcgcccgcg
     1741 agcagcgcga agacgatgac gccgcgccgc ggccgcggcg gcaggcgctt caccagcacg
     1801 tcgacgccga catggatgcc ggtgcgcacg ccgtaagcgg cgccgaactt ggccacccag
     1861 atgaacatgt agacgcacag ctcctgcgcc caggtcatgt cgatccgcgt gacatagtcc
     1921 tggatcgcgg gaatggtgga gagataacgg tggacgacgg cggcgaaggt gatgagcgtc
     1981 gcgcccgcca tcaggctcgc gatcagaatc tcctcgagcc ggtcgaggac ccgcaacggg
     2041 agcttcaaca tggacgagga tggcgacgcg ctgctgctac tgcgtcgcgc cggtgcccgt
     2101 ctccttgtag atcgcctgga tcaggtcctt gccgatgcga ggcgccatct cctcatggac
     2161 cgggagcagg cccttgcgcc actcggcctt ctcctggtcg gagaggacga tgagctcgac
     2221 atcgcccgag gccttgatct tggctagcgc ctcttcgttc tctttctcgg cgatttcgtt
     2281 gcaataaacg gtcgcgtcct tcatcgcgct gtcgagcgcc gcgcggatgt cggccggcag
     2341 cccttcccag aatttcttgt tcacgaccac ggcgtagccg atataggtgt ggtcggacac
     2401 cgtcagatat ttctgcactt cgtagaattt ctgcgtatag ctgttggagg gcgtcgtctc
     2461 cacggcgtcc accacgccgg tctgcagcgc ctggtagacc tccgagaacg ccatgatctg
     2521 gggcagggcg ccgatatggc gataggtggc ctcgagcact ttcgaggact ggatgcgcat
     2581 cttgacgccc ttcaagtcct cgagtttcct gatcggcttg ttcgaggtga tgatcgagaa
     2641 gccgttgtcc cagtaggcga ggccgaggat gcccttgggc tcgagcttct tgaggaggtc
     2701 gcggccgacc ggaccgtcgg tcacgcgcct gagcgcctcg gtcgagggga agatgtaagg
     2761 caggtcgaag gcctcgaact ccttgatccc gagggggccg aacttgctga gggagggcgc
     2821 cagcatctgc acggcgccga gctggagcgc ttcgagctct tccttgtcct tgtagagctg
     2881 gctgttggga tagagctcga cccggacgcg gccatgcgtc cgctcctccg ccagcgcctt
     2941 gaatttctcg gccgccttgc ccttgggcgt gtcgggcgcc acgacatggc tgaacttgat
     3001 gacgatcggc tcttccgccc gtgccggggc gaggccgatc gccaggagca ggccggcgat
     3061 gaccaaggct ggtctcatga acgttccctc cctctaacgg cgctgttttg cgcattctcg
     3121 tctgtattcg acagcgaaat ccagcggaat tgccgttgcg gatcggcgct gatcgagcag
     3181 cctcacgtct cgccgaggaa gcctctgacc gtgtcgatga aagcgtcgag ctgatcgtga
     3241 tgcacccagt ggccggcctt ctcgaaggcg acgacgcggg cggtgcggaa atgatcggcg
     3301 cgcccgtcc
//
LOCUS       DASYNS010000511         2582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1374810, whole genome shotgun sequence.
ACCESSION   DASYNS010000511 DASYNS010000000
VERSION     DASYNS010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374810"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VGP50_15845"
     CDS             <1..768
                     /locus_tag="VGP50_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEV8028906.1"
                     /translation="DGRPTPDPRVFMQFLPRIKQASDAVINITTGGGHGMTVEERLAA
                     PLKASPEMCSLNMGSMNFGLFPMLQRYKDFKYDWERNHLEASRDFIFRNTFKDIERIL
                     SELGEGHGTRFEFECYDVGHLYTLAHFLDRKLVKPPLFVQTIFGILGGIGADPENLTH
                     MKHIADKLFGDDYYWSILAGGRHQLPLVTMGAVMGGNVRVGLEDSLFIGKGQLAKSNA
                     DQVRRIRAILENLSLEIATPKEARAMLALKGGDAVAF"
     gene            784..1488
                     /gene="pcaH"
                     /locus_tag="VGP50_15850"
     CDS             784..1488
                     /gene="pcaH"
                     /locus_tag="VGP50_15850"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEV8028907.1"
                     /translation="MADSTEYERPRPGEQPPYLYADYRGTMKRAPQRALVRLPHTLSE
                     VTGPSFADGWAGADKADLTRQHQTAPIGERMIVAGRVLDEAGRAVPGTLVELWQANAA
                     GRYRHDADQHDAPLDPNFTGAGHGVSDARGEYRFVTIVPGAYPWRNSDNAWRAQHLHF
                     SVFGAAFATRIITQMFFPGDPLLDYDPIFHSIPDAAARERLIASYDPALSVAEWALGY
                     RFDIVLRGRAATPVGL"
     gene            1495..2073
                     /gene="pcaG"
                     /locus_tag="VGP50_15855"
     CDS             1495..2073
                     /gene="pcaG"
                     /locus_tag="VGP50_15855"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEV8028908.1"
                     /translation="MRLVPTASQTAGPYFAIGLAHREWSDLTSGGKAAGPRIRIEGRV
                     LDGDKKPLDDALVEIWQANAAGRYAHPEDRQAKPLDPHFRGFGRCATDKDGRYRFLTV
                     RPGAVPGRGNTLQAPHISLAVFARGLLKQATTRLYFPGEALNNADPVLALVDDARRAT
                     LIAGDAGEESGTRFYRFDIVLQGEGETVFFDV"
     gene            2080..>2582
                     /locus_tag="VGP50_15860"
     CDS             2080..>2582
                     /locus_tag="VGP50_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEV8028909.1"
                     /translation="MTVNPADSPVFGTLFGTDEMRGIFGDRALVQKMLEVEAALARAE
                     ARLGIVPAAAAQAIAAAAAVERIDLTALGESTRHVGYPVVGLTKALARAAGGDAARYV
                     HWGATTQDILDTALVLQIREGLAAVERDLRALAEALAEKADAHRAAVMAGRTHLQHAL
                     PVTFGYK"
BASE COUNT          411 a          938 c          828 g          405 t
ORIGIN      
        1 gacggccggc cgacgcccga tccccgtgtc ttcatgcagt tcctgccgcg catcaagcag
       61 gcatcggacg cggtcatcaa catcaccacc ggcggcggcc acggcatgac ggtcgaggag
      121 cggcttgcgg cgccgctcaa ggcgtcgccc gagatgtgct cgctcaacat gggctcgatg
      181 aatttcggcc tcttcccgat gctgcagcgc tacaaggact tcaaatacga ctgggaacgg
      241 aaccacctcg aggcttcgcg cgatttcatc ttccgcaaca ccttcaagga tatcgagcgc
      301 atcctgagcg agctcggcga gggacacggc acgcgcttcg agttcgaatg ctacgacgtg
      361 ggccatctct acacgctcgc ccatttcctc gaccgcaagc tggtgaagcc gccgctcttc
      421 gtgcagacca ttttcggcat cctcggcggc atcggcgccg atcccgagaa tctcacgcac
      481 atgaagcaca tcgccgacaa gctcttcggc gacgactact attggtcgat cctggccggc
      541 gggcgccatc agctgccgct cgtcaccatg ggcgccgtca tgggcggcaa tgtgcgcgtc
      601 ggccttgagg actcgctctt catcggcaag ggtcagctcg ccaagtccaa tgccgatcag
      661 gtgcgccgca tccgcgccat cctcgagaat ctgtcgctcg agatcgccac gcccaaggag
      721 gcgcgcgcca tgctcgcgct caagggcggc gatgccgtcg ccttctgagg agcgggaaac
      781 accatggctg acagcaccga gtacgagcgg ccgaggccgg gcgagcagcc gccctatctc
      841 tatgccgact accgcgggac gatgaagcgc gcgccgcagc gcgcgctcgt ccgcctgccg
      901 cacacgctct cggaggtgac gggaccgagc ttcgccgatg gctgggccgg agcggacaag
      961 gccgacctga cgcgccagca tcaaacggcg cccatcggcg agcgcatgat cgtcgcgggc
     1021 cgcgtgctcg acgaagcggg gcgcgccgtc cccggcaccc tcgtcgagct ctggcaggcc
     1081 aatgccgccg gccgctatcg ccacgacgcc gatcagcatg atgcgccgct cgaccccaac
     1141 ttcaccggcg ccggtcatgg cgtcagcgac gcgcgcggtg aatatcgctt cgtcaccatc
     1201 gtgccggggg cctatccctg gcgcaacagc gacaatgcct ggcgcgcgca gcatctccat
     1261 ttctcggtgt tcggcgcggc cttcgccacg cgcatcatca cgcagatgtt ctttcccggc
     1321 gatccgctcc tcgattacga cccgatcttc cacagcattc ccgacgcggc cgcgcgcgag
     1381 cggctgatcg cgagctatga cccggcgctc agcgtcgccg aatgggcgct gggctatcgc
     1441 ttcgacatcg tgctgcgcgg ccgcgccgcg acgcccgtgg ggctctagcg cgccatgcgc
     1501 ctcgtcccga ccgcgtcgca gaccgcaggc ccttacttcg cgatcggcct cgctcatcgc
     1561 gaatggagcg atctcaccag tggcggcaag gccgcgggcc cgcgcatccg catcgaggga
     1621 cgcgtgctcg acggcgacaa gaagccgctc gacgacgcgt tggtcgagat ctggcaggcc
     1681 aatgccgccg gtcgctacgc ccatcccgag gatcgccagg ccaagccctt ggatccgcat
     1741 tttcgcggct ttggccgctg cgctaccgac aaggatggcc gttaccgctt tctcaccgta
     1801 cggcccggtg cggtgccggg acgcggcaac accctgcagg cgccgcatat cagcctcgcg
     1861 gtcttcgccc gcggactcct gaagcaagcg acgacgcggc tctattttcc cggcgaggcg
     1921 ctcaacaacg ccgacccggt gctcgccctc gtcgacgatg cgcggcgcgc gacgctgatc
     1981 gccggcgacg ccggcgagga gagcggcacc cgattctatc gcttcgacat cgtgctccaa
     2041 ggcgagggcg agacggtgtt cttcgatgtg tgagcgccca tgaccgtcaa cccggcggat
     2101 tcgccggtct tcggcacgct cttcggcacc gacgagatgc gcggcatctt cggcgatcgc
     2161 gcgctcgtgc agaagatgct ggaggtcgag gcggcgctgg cgcgcgccga ggcgcggctc
     2221 ggtatcgtcc ccgcggccgc ggcgcaggcg atcgcggcgg ccgccgcggt cgagcgcatc
     2281 gacctcacgg cgctcggcga gagcacgcgc catgtgggat atcccgtggt cggcctcacc
     2341 aaggcgctcg cccgcgcggc cggcggcgat gctgcgcgct acgtccattg gggcgccacc
     2401 acgcaggaca ttctcgacac cgccctcgtg ctgcagatcc gcgaagggct ggccgccgtc
     2461 gagcgcgacc tccgcgcgct cgcagaggcg ctggctgaga aggccgacgc ccatcgcgcc
     2521 gccgtcatgg ccgggcgcac acatctgcag cacgctctcc ccgtcacctt cggctacaaa
     2581 tg
//
LOCUS       DASYNS010000512        10085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_906985, whole genome shotgun sequence.
ACCESSION   DASYNS010000512 DASYNS010000000
VERSION     DASYNS010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10085
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906985"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VGP50_15865"
     CDS             complement(<1..1292)
                     /locus_tag="VGP50_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV8028910.1"
                     /translation="MPASVTAGLPAHSRQEVVAAAPAPTRAPRPRWYRSLTAKAVVLA
                     FIFIVVPLFIYDQFRAADEAQNAVFMRSVAEHGHVIGAALAPRLSGERPPLPKLGSEL
                     ARFADDITNVKLLFAPSGTSGFYYVASWPVVAPAQLDVERETLRQQGILDQVSTTCDG
                     DYPVAVRYRGAGGADQVVTSVTPLKMPAGCWVIVTSSAALSLRGTNLGRPYWASPEVR
                     IAAAIYLIMVALVFTTFWSVRRGLKRFTDRARLIRERRSRGGSFGAQNDIPELADVAE
                     EFDHMVEVLDASARDIRRAAEDNAHAFKTPIAVIRQSLEPVKRAIGAENQRGLRAIGL
                     IESSLDKLDGLVASARRLEEATADLMEMPRTDFDLSDLLERLLQAHADVFVERRLRLK
                     GHIAPHVLVHANAEMVETVIENLLDNAVSFSPEGESIGV"
     gene            complement(1255..2058)
                     /locus_tag="VGP50_15870"
     CDS             complement(1255..2058)
                     /locus_tag="VGP50_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV8028911.1"
                     /translation="MNHASNLHSNATTSSSASGAPAPSLPPVRIVLVDDDDSFRESLG
                     LNLIDEGFAVVSFDNGVPALDYFAQGGEADVILLDWRMPGMNGLEVLRRLRRAGATTP
                     VIFLTVLSDDIYEEAALEGGAVDFIDKSRRLSILVKRLHLITEGMRPVPEPDARQEPS
                     DVTRLGRLELRFDINRAAWDGKILDLTLTEFKIVALLAQRAGEDVGYREIYDLVHGKD
                     FVAGYGNEGYRANVRTFIKRIRKKFRDLESEFDQIHNYAGFGYRWIAGP"
     gene            complement(1958..2482)
                     /locus_tag="VGP50_15875"
     CDS             complement(1958..2482)
                     /locus_tag="VGP50_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028912.1"
                     /translation="MAKPVNPLPPRLGAMTALAVSVILAGCNSFPFQIGGTPAPQPVA
                     ASSSLPPPPPHKPATPASPAVVRVPEAPEPEPSSGFDRLIGLDQPQLTVVLGEPAKRA
                     DAPPATIWRYVGQACELEVYFYFDLQSQAMRALHYEVRSHESREQPAQQCYDELVRER
                     RAGAEPAAGTDRPR"
     gene            2648..3334
                     /locus_tag="VGP50_15880"
     CDS             2648..3334
                     /locus_tag="VGP50_15880"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEV8028913.1"
                     /translation="MDRAAAASLAGGASGQFLDLAALRRMPLVTDPFPHIIVPGFIEG
                     PVRAALSQDFPEIQAPGSFAVADLRIGPVFRRFLADLEGPGMRDAIAEKFGIDLDGRP
                     TMVTVRGAARAADGRIHTDTSTKLITVLIYMNETWEAEGGRLRLLRSPDNLDDVVAEV
                     PPAAGTLLAFRVTPHSWHGHAPVTGPRRVVQLNWVTSDAVVRRERLRHGFTTRVKRLL
                     SFKSTSRSIR"
     gene            complement(3477..4355)
                     /locus_tag="VGP50_15885"
     CDS             complement(3477..4355)
                     /locus_tag="VGP50_15885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEV8028914.1"
                     /translation="MAPRPTWKGYLRLSLVSCPIRVYPATTRSERVSFNLLNPKTKNR
                     IEMRTHDAETGEEIPRDTLVRGYEFDKGRYVVVDDDEIDALKIESSETIDLVRFVEEK
                     DIDTIFFNAPYYVAPEGKIATETFRVIQEAMRAEGKAGIGRIVLSSREHAVALAPRHG
                     GMMMMTLRSPEEVRDDAEYFADIEEVPLDKEMVDMARRIIDQKGGEFIPEELTGDRYQ
                     TALRDLVARKVHGEAPSPARPAPGANNVINLMDALKRSLAEEAPKAPAPSRKERAREE
                     KPAARSAPAKRRRTAK"
     gene            4546..7149
                     /gene="ligD"
                     /locus_tag="VGP50_15890"
     CDS             4546..7149
                     /gene="ligD"
                     /locus_tag="VGP50_15890"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF004628.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEV8028915.1"
                     /translation="MNDPLSQYRRKRDFAKTPEPTGGRHKRGAGLRFVVQKHAARRLH
                     YDFRLEMDGVLKSWAIPKGPSLDPQAKRLAVHVEDHPLDYGDFEGVIPQGEYGGGTVI
                     VWDNGIWVPEGDPEAGYRDGVLKFELRGKRLTGRWTLVQMKGREGGKNWLLLKERDEV
                     ARPGSDDAIVAENQTSAVTGRDIKAVAAARDRVWHSNKSDGKDGKGNGADPAPLLDPG
                     KIPGARKAAMPREPGAQLAKVASAVPKGGEWLHEIKLDGYRLLARIRRGKVQLLTRRG
                     NDWTDRFPTLAEAVAALPVEEAIVDGEAVHLLPNGLSSFGALQDDLAREDTSGVIYYL
                     FDLLYLDGYDLTSVRLDLRKEALKTLLAGVPAATLHYSDHVAGQGEAFEARASRMGIE
                     GVVSKRADSPYRATRGGAWLKIKFDRHDELAIIGWTDPSGTRQALGALLLGYYDPEGI
                     LHFAGGVGTGFSDEMLRDLHERLVPLARKAAPSREVAKLAPRRAHWVEPELVAEVRYA
                     NWTGDGRLREPAFIALREDKSGREIVLDPATHSPPAAAVPAMSAPAAPAEPPPTEVSP
                     KTTLEFEGVRLTHAGKVLYPESNITKLDLARYYAIVCDRMLPELAGRPLTLLRAPEGW
                     RDHHFYQKHGNATVPKAVKRVKLAEKEGSAEDIYLFIDDKAGLLSLVQMGVLEIHVWG
                     SRVSDVEHADRVVMDLDPDEGLPWSRVTEGALTLRRMLDDIGLQSFVKATGGKGLHVV
                     VPLRPRHDWVEIKGFAKWLAEELVAREPDGYTAVMAKKKRHGKIFIDYLRNQRGANAV
                     TAYSARARPNAPVAAPLTWHEVEEGMRSDAFTVRTLPARLAALAKDPWEELGRVKQSL
                     SAAVLRKLPAA"
     gene            7231..8328
                     /locus_tag="VGP50_15895"
     CDS             7231..8328
                     /locus_tag="VGP50_15895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8028916.1"
                     /translation="MDLSGAHVAVIGRLGAMPRARIAAAMTRRGAVLTQGLTKAQGLV
                     VGHHAHRRLPPVLAALAAADRRGLWCSSEQRLLRALGLAPPLPSLPRALGLEDLARHS
                     GLDREAVRLLALFDVIDDQDGAFEFRDLVALRQVKRLLDGGSTLGDVIAASAAARPEA
                     SGGNGLAHARLLPLGDGALGRSVGDYLAEIDGQIRLPLDDGGNPSLHQLFAAAASAED
                     EERWGEAEATYRRLLALSPRDGVAHFNLANVLAAQGRQREAEDCLRRAVALEPGLAEA
                     WYNLAHLLESKDDAAAARRCLERALDIDRSFADAIYNLARLRLKAGEPAAAAPLFERY
                     LALDPSSRWAARARQWLRLCQIMQQGAGERR"
     gene            8398..9213
                     /locus_tag="VGP50_15900"
     CDS             8398..9213
                     /locus_tag="VGP50_15900"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEV8028917.1"
                     /translation="MTHQSHGQPKAYHDQRFLDSRDARSLRILAEYLEPLSRFQRHGV
                     QDTIVFMGSARLKSREAAEAALAAAEAGGEGLEAARTAVELSTYYEAARELAHRLTDW
                     SKNLDHDERRFVVCTGGGPGIMEAANRGASEARGLNVGLTISIPNDEFDNPYVSRELH
                     FHFHYFFMRKFWFVYLAKAVVLFPGGFGTLDEMFEILTLVQTRKMRKRVPIVLFGAKF
                     WNEVVNFDALVRYGTISPEDVSLFHRTDSIDEAYEIVTRGLVENALGSPGAQL"
     gene            complement(9330..9704)
                     /locus_tag="VGP50_15905"
     CDS             complement(9330..9704)
                     /locus_tag="VGP50_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8028918.1"
                     /translation="MNARVLVVEDHDLVRSAIEVALSDAGFIVATACHGDDGLEKFQS
                     FHPDLVLTDVMMPHKDGIEMMRAIRELKPQAKVLAMSGYRAGSIDFLSMLQRLGANDV
                     IAKPFDADDLISKVSGCLAAAA"
     gene            9808..>10085
                     /locus_tag="VGP50_15910"
     CDS             9808..>10085
                     /locus_tag="VGP50_15910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028919.1"
                     /translation="MAYATAIRGIVATASAENSRHGLGSLNFPAAKVDVGFWPAGEAV
                     ISSRAQAGWVIGVTCAFALALGWMFFWMAAQSRQIVIDDTYQSSSNLAL"
BASE COUNT         1643 a         3342 c         3422 g         1678 t
ORIGIN      
        1 acgccgatgc tctcgccttc gggcgagaag gagacggcgt tgtcgagcag attctcgatc
       61 accgtctcga ccatctccgc attggcatgc accagcacat gcggcgcgat gtgccccttg
      121 agccggaggc gccgctcgac gaatacatcg gcatgggcct ggagcagccg ctcgagaagg
      181 tcggagagat cgaagtcggt gcgcggcatc tccatgagat cggcggtggc ctcctcaagc
      241 cggcgcgccg aggcgacgag gccgtcgagc ttgtcgagcg agctctcgat gaggccgatg
      301 gcgcgcaagc cgcgctggtt ctccgcgccg atcgcgcgct tcaccggctc gagcgactgg
      361 cggatgacgg cgatcggcgt cttgaacgcg tgcgcgttgt cttcggcggc gcggcggatg
      421 tcgcgtgccg aggcgtcgag gacttcgacc atgtgatcga attcctcggc gacatcggcg
      481 agctcgggaa tgtcgttctg cgcgccgaag gagccgccgc gcgagcgccg ctcgcggatc
      541 agccgcgcgc ggtcggtgaa gcgcttcaat cctcgccgca cgctccagaa ggtggtgaag
      601 acgagcgcca ccatgatcag atagatcgcg gcggcgatgc gcacttcggg gcttgcccaa
      661 taggggcggc cgagattggt gccgcgcagc gacaacgccg ccgacgacgt gacgatcacc
      721 cagcagccgg ccggcatctt gagcggcgtg accgaggtca cgacctgatc ggcgccgccg
      781 gcgccgcgat agcgcaccgc gacgggataa tcgccgtcgc aggtggtcga gacctggtcg
      841 aggatgcctt gctggcggag cgtctcgcgc tcgacgtcga gctgcgccgg cgccaccacg
      901 ggccaggagg cgacgtagta gaagccggac gtcccggacg gagcgaagag cagcttcaca
      961 ttggtgatgt catcggcaaa gcgcgcgagc tcgctgccga gcttgggcag cggcggccgc
     1021 tcgcccgaaa gccgcggcgc gagcgcggcg ccgatcacat gaccatgctc ggcgacgctg
     1081 cgcatgaaga cggcgttctg cgcctcatcg gcggcgcgga actggtcgta aatgaagagc
     1141 ggcacgacga tgaagataaa cgccagcacc acggccttgg ccgtgagcga gcggtaccat
     1201 cgcggtcgcg gcgctcgcgt cggcgccggc gcggcagcga cgacctcctg ccggctatgg
     1261 gccggcaatc cagcggtaac cgaagccggc atagttgtgg atctgatcga attccgactc
     1321 gagatcgcgg aacttcttgc ggatgcgctt gatgaaggtg cggacattgg cgcgatagcc
     1381 ttcgttgccg tagccggcca cgaagtcctt gccatgcacc aggtcgtaga tctcgcgata
     1441 gccgacatcc tcgccggcgc gctgcgcgag cagcgccacg atcttgaact cggtcaaggt
     1501 caggtcgagg atcttgccgt cccaggcggc gcggttgatg tcgaagcgca gctcgagccg
     1561 gccgaggcgg gtgacgtcgc tcggctcttg gcgcgcatcg ggctcgggaa ccggtcgcat
     1621 gccctcggtg atgagatgca ggcgcttcac gaggatcgac agccgccgcg acttgtcgat
     1681 gaagtcgacg gcgccgcctt cgagcgccgc ctcttcatag atgtcgtcgc tgagcacggt
     1741 gaggaagatg acgggcgtcg tcgcgccggc gcggcgcagg cggcgcagca cttcgagccc
     1801 gttcatcccg ggcatgcgcc agtcgagcag gatgacgtcg gcctcgccgc cttgcgcgaa
     1861 atagtcgagc gccggcacgc cgttgtcgaa gctgacgacg gcaaaaccct cgtcgatcag
     1921 attgagaccg agcgactcgc ggaacgagtc gtcgtcgtca acgaggacga tccgtaccgg
     1981 cggcaggctc ggcgccggcg cgccgctcgc ggacgagctc gtcgtagcat tgctgtgcag
     2041 gttgctcgcg tgattcatgg ctcctcactt cgtaatggag ggcgcgcatc gcctggctct
     2101 gcagatcgaa atagaaatag acctcgagct cgcacgcttg tccgacatag cgccagatgg
     2161 tcgccggcgg ggcgtcggcg cgctttgccg gctcgcccag caccaccgtc agctgcggct
     2221 ggtcgaggcc gatcagccgg tcgaagccgc tcgaaggctc cggctccggc gcctccggca
     2281 cccggacgac ggccgggctc gcgggcgtcg ccggcttgtg cggcggcggc ggcgggagcg
     2341 aggatgaagc tgccacgggc tgcggggcag gggtgccgcc gatctgaaag ggaaagctgt
     2401 tgcatccggc cagtatgacg gatacggcga gcgccgtcat ggccccgaga cggggcggga
     2461 ggggattcac tggtttcgcc atggcacgct gcccgatgcc cttcctggat gccgtcacat
     2521 cgccacaagg gcggtcaaca tagagttaac gaaacgcgcg ggtacgctat cgattgcgct
     2581 ctgacgacgg tttcgccatg gttccacgtc cgggcgtttt atggcaaggt cggaacggaa
     2641 aaagaccatg gatcgggcag cagcagcaag cttggcgggt ggcgcgtcgg ggcagttcct
     2701 cgacctcgcg gcgttgcgcc gcatgccgct cgtcacggat cccttccctc atatcatcgt
     2761 ccccggcttc atcgaaggcc cggtgcgcgc cgcgctgagc caggatttcc cggagatcca
     2821 ggcccccggc agcttcgccg tcgcggatct tcggatcggc ccggtcttca gacgcttcct
     2881 cgcggacctc gaggggccgg ggatgcgcga cgccatcgcc gagaagttcg ggatcgatct
     2941 cgacgggcgg ccgaccatgg tgacggtgcg cggcgccgcc cgggccgccg acgggcgcat
     3001 tcacaccgac accagcacga agctcatcac cgtgctgatt tacatgaatg agacatggga
     3061 agccgagggc ggccgtctca gactcttgcg ctcgcccgac aatctcgacg acgtcgtcgc
     3121 cgaagtgccg ccggccgcag gaacactgct agccttccgc gtgacgcccc attcctggca
     3181 cggccacgcg ccggtgaccg ggccgcgccg agtcgtgcag ctcaactggg tcacgagcga
     3241 cgccgtggtc cgtcgcgagc ggctgcgcca cggctttacc acacgggtca agcggctgct
     3301 gtctttcaag agcacatctc gttcaattag ataaatgtcg tcgtccccgc gaaagcgggg
     3361 acccagggcg agcgacgtgc cggcggcccc tggatccccg ctttcgcggg gacgacgaag
     3421 aaaattgttc taaagggatg tgctgtggcc ggacgaacgc tacgcgtcgc gcggcgctat
     3481 ttcgctgtgc gccggcgttt cgcaggcgcg ctgcgcgcgg ccggcttttc ctcgcgggcg
     3541 cgctccttgc gactcggcgc cggcgctttg ggcgcttcct cggcgaggct gcgcttcaat
     3601 gcgtccatga ggttgatgac gttgttggct ccgggcgcgg gacgtgccgg cgacggcgcc
     3661 tcgccatgga ccttgcgggc gaccaggtcg cgcaaggccg tctgatagcg gtcccccgtc
     3721 agctcctcgg ggatgaactc gccgcctttc tggtcgatga tgcggcgcgc catgtcgacc
     3781 atctccttgt cgagcggcac ctcttcgata tcggcaaagt attcggcgtc gtcgcgcacc
     3841 tcttccggag agcgcagcgt catcatcatc atgccgccat ggcgcggcgc cagcgcgacc
     3901 gcgtgctcgc gcgaggacag cacgatgcgg ccgatccccg ccttgccttc cgcgcgcatc
     3961 gcttcctgga tgacgcggaa ggtctcggta gcgatcttgc cctcgggcgc cacgtaatag
     4021 ggcgcgttga agaagatcgt gtcgatgtcc ttctcctcga cgaagcgcac gaggtcgatg
     4081 gtctccgagg attcgatctt caacgcatcg atctcgtcgt cgtcgaccac gacataccgc
     4141 cccttgtcga attcgtagcc ccgaaccagc gtatcgcgcg ggatctcttc gcctgtctcg
     4201 gcgtcatgcg tgcgcatctc gatgcggttc ttggtcttgg gattgagcag attgaacgag
     4261 acccgctcgc tgcgcgtcgt tgccggatag acccggatcg ggcaggagac cagcgacagg
     4321 cggagataac ccttccaagt cggacgcgga gccattgtct tcccctacct tggccggacc
     4381 gaggcgccgg cacttggttc gacgcgaata atcccctcga atttgcataa caaacagtaa
     4441 agaagcgcgg caatccccag gttgcatatt aagacgcgtc gcccgacaat tggcaactcc
     4501 aggtcttccg ccgtcgttta gagggcctga gacaggatcc gtgccgtgaa cgacccgctc
     4561 tcgcaatacc gccgcaagcg cgatttcgcc aagacgccgg agccgacggg cggccggcac
     4621 aagcgcggcg cgggattgcg cttcgtcgtg caaaagcacg cggcgcggcg cctccattac
     4681 gacttccgcc tggagatgga cggcgtcctc aagagctggg cgatcccgaa agggccgagc
     4741 ctcgatccgc aggccaagcg gctcgccgtt catgtcgagg atcatccgct cgactatggc
     4801 gacttcgaag gcgtgatccc gcaaggggaa tatggcggcg gcacggtcat cgtctgggac
     4861 aacggcatct gggtccccga gggcgatccc gaggccggct atcgcgacgg cgtcctcaaa
     4921 tttgagctgc gcggcaagcg cctcaccgga cgctggacgc tcgtccagat gaaaggccgc
     4981 gaaggcggca agaactggct gctgctcaag gagcgggacg aggtggcgcg gccgggcagc
     5041 gacgacgcga tcgtcgccga gaaccagacc agcgccgtca ccggccgcga catcaaggcc
     5101 gtcgccgctg cgcgcgaccg cgtctggcat tcgaacaaaa gcgacggcaa agacggcaaa
     5161 ggcaacggcg ccgacccggc gccgcttctc gatccgggca agatccccgg tgcccgcaag
     5221 gcggcgatgc cgcgcgagcc cggcgcgcag ctggccaagg tcgcgagcgc cgtgcccaag
     5281 ggcggcgagt ggctgcacga gatcaagctc gacggctatc gcctgctcgc ccgcatacgg
     5341 cgcggcaagg tgcagcttct gacccgccgc ggcaacgact ggaccgaccg cttcccgacc
     5401 ctggccgaag ccgtcgcggc cctgccggtc gaggaggcga tcgtcgacgg cgaggccgtg
     5461 catcttctac ccaacggctt gagctccttc ggcgcgctcc aggacgatct cgcgcgcgag
     5521 gacacctcgg gcgtgatcta ttacctcttc gatctcctct atctcgacgg ctacgacctc
     5581 acctccgtcc gtctcgatct gcgcaaagag gcgctgaaga cgctgctcgc cggcgtgccc
     5641 gccgcgacgc tccattacag cgaccatgtg gccgggcagg gcgaggcctt cgaggcgcgc
     5701 gcttcacgaa tgggcatcga aggcgtcgtc tcgaagcgcg ccgactcgcc ttatcgcgcg
     5761 acccggggcg gcgcctggct caagatcaaa ttcgatcgcc acgacgagtt ggcgatcatc
     5821 ggctggaccg atccgagcgg cacccgccag gcgctgggcg cgctgctcct cggctattac
     5881 gaccccgagg ggattctgca tttcgccggc ggcgtcggca cgggattctc ggacgagatg
     5941 ctgcgtgacc tgcatgagcg cctggtgccg ctcgcgcgga aggctgcgcc gtcgcgcgag
     6001 gtcgcgaagc tggcgccgcg tcgcgcccat tgggtcgagc ccgaattggt cgcggaggtg
     6061 cgctatgcca attggacggg cgacggccgg ctgcgcgagc ctgccttcat cgccttgcgc
     6121 gaggacaaga gcggacgcga gatcgtgctc gaccccgcga cccatagccc acccgcggcg
     6181 gcggtgccgg ccatgagcgc tccggccgcg cccgccgagc cgccgccgac ggaggtttcg
     6241 cccaagacca cgctcgaatt cgaaggcgtg cggctgaccc atgccggcaa ggtgctttac
     6301 cccgagagca acatcaccaa gctcgatctc gcgcgctact acgccatcgt ctgcgatcgc
     6361 atgctgccgg agctcgccgg ccggccgctg acgctgctgc gcgcgcccga gggctggcgc
     6421 gatcaccatt tctatcagaa gcacggcaac gccaccgtgc ccaaggcagt gaagcgcgtg
     6481 aagctcgccg agaaagaggg tagtgccgaa gacatctatc tcttcatcga cgacaaggcc
     6541 gggctcttgt cgctggtgca gatgggcgtg ctcgagatcc atgtctgggg ctcgcgcgtc
     6601 agcgacgtcg agcatgccga ccgtgtcgtc atggatctcg atcccgatga aggattgccc
     6661 tggtcgcgtg tcaccgaggg ggcgctgacg ctccgccgca tgctcgacga catcgggctg
     6721 caaagcttcg tcaaggcgac gggcgggaag gggctccatg tcgtcgtgcc gctgcgcccg
     6781 cgccacgact gggtggagat caaggggttc gccaaatggc tcgccgagga gctcgtcgcg
     6841 cgcgagcccg acggctacac ggcggtcatg gccaagaaaa agcggcacgg caagatcttc
     6901 atcgattatc tgcgcaatca gcggggcgcc aacgccgtca ccgcctattc cgcccgcgcc
     6961 cggcccaatg cccccgtcgc ggcgccgctg acctggcacg aggtggagga gggcatgcgc
     7021 tccgacgcct tcacggtgcg gaccttgccc gcgcgcctcg ccgcgctcgc caaggacccc
     7081 tgggaagagc tcggccgcgt caagcaatcg ctttcagccg ccgtgctgcg caaattgccg
     7141 gccgcctgag cgagcggcgt gcccggcgcg tgggggtgcg ctgatgcgcg ttatgcgctc
     7201 gcggccgaga acgcgatata gtggccggcg atggatctgt cgggcgcgca tgtggcggtg
     7261 atcgggcggc tgggcgccat gcctcgcgcg cgcatcgccg ctgccatgac gcggcgcggc
     7321 gcagtgctca cccagggcct gacgaaggcg caaggactcg tcgtcggcca tcacgctcac
     7381 cgccgtctgc cgcccgtgct tgctgcgttg gcggcggccg accgccgcgg cctctggtgc
     7441 tcgagcgagc agcggctctt gcgtgccttg ggactggcgc cgccgctgcc gtcgctgccg
     7501 cgcgccctcg gcctcgagga cctcgcccgc cactccggcc tcgaccgcga ggcggttcgg
     7561 ctgttggcgc tgtttgacgt gatcgacgat caggacggcg ctttcgaatt ccgcgatctc
     7621 gtggctctcc gccaagtgaa gcggctgctc gacggcggca gcacgctcgg cgatgtcatc
     7681 gccgccagcg ctgcggcgcg gcccgaggcg tcgggcggca acgggctcgc gcatgcgcgg
     7741 ctgctgccgc tcggggacgg cgcccttggc cgcagcgtcg gcgactatct cgccgagatc
     7801 gacggccaga tacggttgcc gctcgatgat ggcggcaacc cctccctcca ccagcttttc
     7861 gcggcggcag ccagcgccga ggacgaagag cggtggggcg aagccgaggc gacctatcgc
     7921 cggctcctgg cgctctcgcc gcgtgacggc gtcgcgcatt tcaacctcgc caacgtgctg
     7981 gcggcccagg gccgccagcg ggaagccgag gattgcctgc gccgcgcggt ggcgctcgag
     8041 cccgggctcg ccgaagcgtg gtacaatctc gcgcatctgc tcgagagcaa ggacgatgca
     8101 gccgcggcgc ggcgttgcct ggagcgggcg ctcgacatcg accgcagctt tgccgatgcg
     8161 atctacaatc tggcgcggct tcgcctgaag gcgggggagc cggcagcggc ggcgccgctc
     8221 ttcgagcgct atctggcgct cgatccgtcg tcgcgctggg cggcgcgggc gcggcagtgg
     8281 ctgcggctct gccagatcat gcagcaaggc gccggcgagc ggcggtgagc gccgcggcgc
     8341 gtttagacgt tattcaccaa gccgcagcat actcccgccc ttttttcgag gtggaacttg
     8401 actcaccaat cgcacggcca gcccaaggcc tatcacgacc aacgctttct cgacagccgc
     8461 gacgcgcgtt cgctgcgcat cctcgccgaa tatctcgaac cgctctcgcg gttccaacgc
     8521 catggcgtgc aggacacgat cgtcttcatg ggctcggcgc gcctcaagtc gcgcgaagcc
     8581 gcggaagccg cgctcgccgc tgccgaggcc ggcggcgaag ggttggaggc ggcgcggacg
     8641 gcggtcgagc tctcgaccta ttacgaggcg gcacgcgagc tcgcgcatcg tctcaccgac
     8701 tggtcgaaga atctcgatca cgacgagcga cgcttcgtcg tctgcaccgg cggcggcccc
     8761 ggtatcatgg aagctgccaa tcgcggcgcc tcggaggcgc ggggcctcaa tgtcgggctc
     8821 accatctcga tccccaatga cgagttcgac aatccctatg tgagccgcga gctgcacttc
     8881 cactttcact atttcttcat gcggaagttc tggttcgtct atctcgccaa ggccgtggtg
     8941 ctgttccccg gcggcttcgg aacgctcgac gagatgttcg agatcctgac gctggtgcag
     9001 acgcgcaaga tgcgcaagcg cgtgcccatc gtgctgttcg gcgccaaatt ctggaacgag
     9061 gtcgtcaact tcgacgcgct ggtgcgctac gggaccatca gccccgagga cgtctcgctg
     9121 ttccaccgca cggattcgat cgacgaagct tacgagatcg tcacccgcgg tctcgtcgag
     9181 aacgcgctgg gatcgcccgg ggcgcagctg tagttaggaa gttgtcagtt atcagttgtc
     9241 agttatcagg tcgcggcggc ggtccagctg tcgctcgata ggaccaccgt cggacgactg
     9301 ataactgata actgacaact gacgacttcc taagcggccg cggcgaggca gccgctcacc
     9361 ttcgagatca ggtcgtcggc gtcgaaaggc ttggcgatca cgtcattggc gccgaggcgc
     9421 tgcagcatcg agagaaaatc gatgctgccg gcgcgatagc cggacatggc caggaccttg
     9481 gcctgcggct tgagctcgcg gatggcgcgc atcatctcga tgccgtcctt gtgcggcatc
     9541 atcacgtcgg tgagcacgag atcggggtgg aagctctgga atttctccag gccgtcgtcg
     9601 ccatggcagg cggtcgcgac gatgaagccg gcatcgctga gggccacctc gatggcagac
     9661 ctgacgaggt cgtggtcctc gaccaccaga acgcgcgcgt tcatgttgac cgtcccttgc
     9721 tgtgccggag catgcttata gtaggattgc gcgtcgcgcg ttagtcggcg cggcgccatc
     9781 ggtgaagtgg cggaccggct atgccgaatg gcatatgcca ccgctattcg gggcatcgtc
     9841 gcgaccgctt ccgccgaaaa ctcacgacat ggtttaggca gccttaactt cccggctgct
     9901 aaagtggacg tcggcttctg gcctgcaggg gaggctgtta tttcatcgcg cgcccaagcg
     9961 ggttgggtca tcggcgtcac ctgcgctttc gcgctggcgc tgggctggat gttcttctgg
    10021 atggccgccc agtcgcgcca gatcgtcatc gatgacacct accagtcgag ctcgaacctc
    10081 gcgct
//
LOCUS       DASYNS010000513         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_128818, whole genome shotgun sequence.
ACCESSION   DASYNS010000513 DASYNS010000000
VERSION     DASYNS010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128818"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1541
                     /locus_tag="VGP50_15915"
     CDS             99..1541
                     /locus_tag="VGP50_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEV8028920.1"
                     /translation="MRFASLKKRLVRLLPLAALAVLIPLTFAAAQQAAAPDQAAPAAA
                     AAQAAAPPACAGDPDPKKAVLENCTPNSGDTAWMLTSVALVLMMTIPGLGLYYGGLVR
                     KKNVGDTVMTSFAITCLVTVLFAICTYSMAFRAGTPFLGGFDRAFLQGIVSDIGKGIG
                     NPNPLAPTIPETVYMCFQMTFAIITPALIAGAFAERMKFSAMLWFIGLWAVVVYAPVA
                     HWVWGPDGFLNSGNGDAWVKVLDFAGGTVVHVNSGVAGLACALMLGKRKDTGPAHNVV
                     LTFIGASLLWVGWFGFNAGSAVTANLQAGMAMTVTQIATAVAGLTWMFVEWAARGKPT
                     VVGICTGAVAGLVAITPASGFVGPIGSMVIGVAAGVVCYLGAMTVKSFFGYDDALDAF
                     GVHAVGGATGAILTGVFAINEYGGTPGLIEGNAGQVVNQLIGVGIVIAYDFIASLIIL
                     KVVDLVIGLRVDVEIEREGLDLTLHGETVQ"
     gene            complement(1707..1877)
                     /locus_tag="VGP50_15920"
     CDS             complement(1707..1877)
                     /locus_tag="VGP50_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEV8028921.1"
                     /translation="MTRIVVRLLRDESGATAIEYGLIAALISVVIIGAVTLVGTNLSS
                     VFNSIATALSAA"
     gene            2115..>3691
                     /locus_tag="VGP50_15925"
     CDS             2115..>3691
                     /locus_tag="VGP50_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEV8028922.1"
                     /translation="MAMAAARGAVGAGRVERFRHVFEGLGFLLLAASVLLALALASYD
                     HGDPSWNHAIDGAPRNALGPSGAVLSDLLLQGLGIAASLIPIILLYWSVRLLLGRGLA
                     WFWLRLLLMPAALCFATLALAPIPAPRAWPIAAGLGGVLGRLMSRRLADIGVAPVLTA
                     LAAAVLAGLLLVYAMGFSLDDWSNVESADRARRQRRRPRPLRWAALPVVGLQRLLGAL
                     AWLRRDAAVEPRREPSIDQPAVPTGGGEAEEETLARERAARRRQQSVPVVPSKAGARR
                     PAVRQAALDLGPAGRHLLPPLDLLTAPPPPARTAAINEEALQQNARLLESVLEDFGVR
                     GQIVKVRPGPVVTLYELEPAPGIKASRIIGLADDIARSMSAISARVAVVPGRNVIGIE
                     LPNAKAETVYLRELLTSEAYERAASRLTLVLGKDIGGAPVIGDLARMPHLLIAGTTGS
                     GKSVGINTMILSILYRLPPEQCRFIMIDPKMLELSVYDGIPHLLCPVVTDPRKAVVAL
                     KWTVREMESRYRAMSKLG"
BASE COUNT          526 a         1272 c         1200 g          693 t
ORIGIN      
        1 ggcgcgacct tcctccctgg catgattctt gagttcagcg acccggccgc cgggcgaatc
       61 ggcccggccg gcatgctcgt tttagcggga ggtagctcat gcgttttgcg tctttgaaaa
      121 agcggctggt gcgtctgttg ccgctggccg cgcttgcggt cctgatacct ttgactttcg
      181 ccgcggccca acaggcggcg gcgcctgacc aggcggcgcc tgccgccgct gcggcacaag
      241 cggccgcgcc gccggcctgc gccggcgatc ccgatccgaa gaaagcggtg ctggagaact
      301 gcacgcccaa ttccggcgac accgcctgga tgctgacctc cgtggcgctg gtgctgatga
      361 tgaccattcc cggactcggc ctctattacg gcggcttggt ccgcaagaag aatgtcggcg
      421 acaccgtgat gacgagcttc gccatcacct gcctcgtcac cgtgctcttt gcgatctgca
      481 cctacagcat ggccttccgc gccggcacgc cgttcctcgg cggcttcgat cgcgccttcc
      541 tgcaaggcat cgtcagcgac atcggcaaag gcatcggcaa tcccaatccg ctggcgccga
      601 ccatccccga aaccgtctac atgtgcttcc agatgacctt cgcgatcatc actccggcgc
      661 tcattgccgg cgccttcgcg gagcgcatga agttctcggc gatgctgtgg ttcatcggcc
      721 tctgggcggt ggtggtctat gcccccgtcg cgcattgggt gtggggaccg gacggcttct
      781 tgaacagcgg caacggcgac gcctgggtca aggtgctcga tttcgccggc ggcacggtgg
      841 tgcatgtgaa ttcgggcgtg gccgggctcg cctgcgcgct gatgctgggc aaacgcaagg
      901 acacgggtcc cgcgcataac gtcgtgctga ccttcatcgg cgcgtcgctg ctctgggtcg
      961 gctggttcgg cttcaacgcc ggctccgcgg tcaccgcgaa tctgcaggcg ggcatggcga
     1021 tgaccgtgac gcagatcgcc accgccgtcg ccgggctcac ctggatgttc gtggagtggg
     1081 cggcgcgcgg caagccgacc gtcgtcggca tctgcaccgg cgcggtcgcc gggctcgtgg
     1141 ccatcacccc ggcctcgggc ttcgtcggcc cgatcggctc catggtcatt ggcgtcgccg
     1201 ccggcgtcgt ctgctatctc ggcgcaatga cggtgaaaag cttcttcggc tatgacgacg
     1261 cgctcgacgc cttcggcgtc catgcggtcg gcggcgccac cggcgccatc cttaccggcg
     1321 tcttcgccat caacgagtat ggcggcacgc ccggcctcat cgagggcaac gccgggcagg
     1381 tcgtcaatca gctgatcggc gtcggcatcg tcatcgccta cgacttcatc gccagcctga
     1441 tcatcctcaa ggtcgtcgac ctcgtgatcg gtctgcgtgt cgatgtcgag atcgagcgcg
     1501 aaggcctcga cctcacgctt cacggcgaga ccgttcagta aacgtcagga atcctcccgt
     1561 ggcggccggg ggtgcctttg ggcacccccg tgccttttca gggatggctc gcgtttttcg
     1621 gacactattt tttgattaaa gagtatcagg gcgcgcccac gcgcttgacc gcgcgcgcct
     1681 ctctcacatc accaggaaat acgtcttcac gccgccgaca aggcagtggc gatgctgttg
     1741 aacaccgagc tcagattggt gccgaccagc gtcaccgcac cgatgatcac gaccgaaatg
     1801 agcgcagcga tcaggccata ttcaatggcc gtcgcgccgc tctcgtcgcg caacaaccga
     1861 actacgatcc gcgtcatacc cgcctcctga aaaaagcccc atgacctaga acgtagcctg
     1921 gactaacgat cctggcgagt tttatcgccg ctgatttggg ccaaagcctt gatattgctg
     1981 tcaaagcttc ccccgagagg caatttccat ggaatctccg ggccttggga tccgccctgc
     2041 tcttggcgat gccgcgtctc gctcgtatga tgttgccgct ttgtgccggt ggggcggccg
     2101 tgaggagagg gcagatggcg atggcggcag cgcgcggcgc ggtcggagca ggacgggtcg
     2161 agcgcttccg ccatgtgttc gaaggtctgg gattcctgct gctcgccgct tcggtcctgc
     2221 tggcgctggc gctcgcgagt tacgaccacg gcgatccctc ctggaaccat gccatcgatg
     2281 gcgcgccgcg caatgctctg gggccttcgg gagcggtgct ctccgacctg cttctgcaag
     2341 gcctgggcat tgctgcgtcg ctgatcccga tcattctcct ctactggtcg gtgcgcctgc
     2401 tgctcgggcg cggcctcgcc tggttctggt tgcgcctcct cctcatgccc gccgcgcttt
     2461 gcttcgccac gctggcgctc gcgccgatcc cggcaccccg cgcctggcca atcgccgcgg
     2521 gcttgggcgg cgtgctcggc cggctgatgt cgcggcgcct cgccgacatc ggcgtcgcgc
     2581 ccgtgctcac ggccctcgcc gcggccgtgt tggccggcct gctcctcgtc tacgccatgg
     2641 gcttctccct cgacgactgg tcgaatgtcg aatcggcgga ccgcgcgcgc cggcagcgcc
     2701 ggcggccacg gcctctgcgc tgggcagcac tgcccgtcgt cggtcttcag aggctgttgg
     2761 gtgcgctggc ctggctgcgg cgggacgcgg ccgtcgaacc gcgccgcgag cccagcatcg
     2821 atcagccagc agttcccacc ggaggagggg aggcggagga ggagaccctg gcgcgcgagc
     2881 gcgcggcgcg gcgccgccag cagtcggtgc cggtggtgcc gtccaaggct ggtgcgcggc
     2941 ggccggcggt gcgccaggcg gcgctcgatc ttggtcccgc cggccggcac ttgttgccac
     3001 cgctcgacct gctgaccgcg cccccgccac cggcgcgtac cgctgccatc aacgaggaag
     3061 cgctgcagca gaatgcgcgg ctgctcgaat ccgtgctcga ggatttcggc gtccgcggcc
     3121 agatcgtgaa ggtccggccg ggccctgtgg tgacgctcta cgagctcgag ccggcgcccg
     3181 gcatcaaggc ctcgcggatc atcggccttg ccgacgacat cgcgcgctcg atgagcgcca
     3241 tctcggcgcg cgtggcggtc gtccccggac gcaacgtcat cggcatcgag ctgcccaacg
     3301 ccaaggctga gaccgtctac ctgcgcgagc ttctgacctc ggaggcctat gagagggccg
     3361 cgtcgcggct cactttggtt ctcggcaagg acatcggcgg cgcgccggtg atcggcgatc
     3421 tcgcgcgcat gccgcatctg ctcatcgccg gcaccaccgg ctcgggcaag tcggtcggca
     3481 tcaacaccat gattctctcg atcctctatc gcctgccacc cgagcaatgc cgcttcatca
     3541 tgatcgatcc gaagatgctg gagctctcgg tctatgacgg catcccgcat ctcctttgcc
     3601 cggtcgtgac cgacccgcgc aaagcggtgg tcgcgctcaa atggacggtg cgcgagatgg
     3661 agagccgcta tcgcgccatg tcgaagctcg g
//
LOCUS       DASYNS010000514         4194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_286335, whole genome shotgun sequence.
ACCESSION   DASYNS010000514 DASYNS010000000
VERSION     DASYNS010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286335"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..1895
                     /locus_tag="VGP50_15930"
     CDS             309..1895
                     /locus_tag="VGP50_15930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8028923.1"
                     /translation="MAAAPQVPVAEPSPAQVRLFLLGLGLATGMEFYSNDSMNLILPD
                     IAGTIGVSFDEASWLLTVYSCSLFFGVPVSIWMERRFGYRRFLIGTIIVFAVTAAAGP
                     FAPDFATMLLARAIQGFAGAGLYVWWRASIYMLLSKAQRSASLMRVSTMLYLSSAAGL
                     LASGYITDHFNWRLAFLPNLVYAVGAIALLLRSIPSTAASAPAARADRTDVIGIALLA
                     LALVSLQIALNRGQIDDWFDSAFIQRLAWTSVVAAVLFAFWQTSAHNGAPLLQFDLLR
                     DRRVLSSALIGIFSGIILSGSLYALPEFLRNIDPEPHSATRTGQVMCAYALAAAAIRP
                     LVLPLVARIGQRKTIAFALVMLVASMLLFVRLLTTGTPDAAYIAPLVLYAFCLAPLLP
                     AVGSGTVGRVEQARLLDGVSLYMTFRQFGASIGVAVLTILIEQRETLHSSRLFEHLRT
                     ASVTTGDWLLRATAIIASRDGESAIESSHMATKLLAVAGSRQAATLAYADAFLFMALI
                     GVAALCLVPIIPPTPTEPKK"
     gene            1892..3067
                     /locus_tag="VGP50_15935"
     CDS             1892..3067
                     /locus_tag="VGP50_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV8028924.1"
                     /translation="MSTAASDKATPGRAAEPVGAPASARGDAAIEVRRVVEAPPQPRV
                     QRSRRSLVLVAAAAALVAAALYIYVPRLFGVATDDAYVDAHVVSVVPKVSAYVVALHV
                     DDNTNVRAGELLVDLDPRDFEVAVASAVADLASAEASAANIEAQTTEQQALIAQSEAT
                     LEGDRAVVAFAQQQLARYTNLANSGVGSVERLQQAQSDIGERKAVLERDRAAVEAART
                     HLAVLETARRQADANIERQRAALDQARLNLSYTQISAPADGTIANKTVQVGNFVQPGQ
                     ILFSMVPDQLYVTANFKETQLTNVRPGQSAVVRVDAFPGLRLSGRVDSMQRGTGAQFA
                     LLPPENATGNFVKVVQRVPVKIILDDPGEALRYISPGMSVEATIHFAKPPRWLAFLE"
     gene            complement(3108..>4194)
                     /locus_tag="VGP50_15940"
     CDS             complement(3108..>4194)
                     /locus_tag="VGP50_15940"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV8028925.1"
                     /translation="AIKAALARARLRPEDIATVALGNVIQAGVKMNPARQAAIHAGLP
                     VSVPAMTVNRVCGSGAQAIVSAAQEILLGSVDVAIAGGMENMDLAPYLIARGRWGYRM
                     GDGQLYDSMLRDGLNDAFSDEHSGWHTEDLAEKFQIGREAQDQWALRSQQRFSAAQAA
                     GKFTAEIAAVDVPGRKGPIIVDKDEHNRPNTTLEALAALKPAFRKDGTITAGNAPGVN
                     SGASAMVLADRQWAEARGLAPVARLVSYGIAAVEPGMFGLGPIPAVRHALERARWKIA
                     DIERIEINEAFAAIVIAVTRELGLSPDIVNVEGGAIAHGHPIGATGAVLTTRLIHSMR
                     RDGLRRGVVTLCIGGGQGIALALETIA"
BASE COUNT          593 a         1492 c         1380 g          729 t
ORIGIN      
        1 gcgcggtaac tatactttcg tttatctctc ctcggcctgg tgaatacttg tagcgctaag
       61 aagcgccgcg catcgtccga tggcgcttcc cgtcgcgaat ctcgggcgcg ctcggttgca
      121 gctgctgggc cttttaaagg cggggggacg ccatccatgc tccaagcggc aatcccttac
      181 ggcacgctcg cggcgcggct ttgccccgcc gccctggcgc tcgtcgccct tcacgtgctg
      241 tcgcccgaga gctgaccgtg gggaacggct cgaccctgtc accgtccctg gggatcgccg
      301 cccctcgggt ggcggccgcg cctcaggtcc ccgtagccga gccatctccg gcgcaggtcc
      361 ggctgttcct gctcggcctc gggcttgcga ccgggatgga gttctacagc aacgacagca
      421 tgaacctcat cctcccggac atcgccggga cgatcggcgt ctccttcgac gaagcgagct
      481 ggctgctcac ggtctatagc tgctcgctct tcttcggcgt cccagtatcg atctggatgg
      541 agcgccgctt cggctaccgc cgcttcctca tcgggacgat cattgttttt gccgtgacgg
      601 ccgcggccgg tccttttgcg ccggacttcg caaccatgct gctcgcgcgg gccatccagg
      661 gctttgccgg ggccggcctc tacgtctggt ggcgagcgag catctatatg ctgctgtcca
      721 aggcgcagcg cagcgcctcg ctgatgcggg tctcgacgat gctctatctc tcgagcgctg
      781 ccgggctgct ggcgagcggc tacatcaccg accacttcaa ctggcggctc gccttcctgc
      841 ccaacctcgt ctatgcggtg ggcgcgatcg cgctgctcct gcgcagcatc ccttccaccg
      901 cggcaagcgc gccggcggcg cgcgccgatc gcacggacgt catcggcatc gcgctcctcg
      961 cgcttgcgct cgtgtcgctg cagatcgcgc tcaaccgcgg ccagatcgac gactggttcg
     1021 actctgcctt catccagcga ctcgcctgga ccagcgtcgt cgccgccgtc cttttcgcct
     1081 tctggcagac gagcgcgcat aatggtgcgc cgctgctgca gttcgacctg ctccgcgacc
     1141 gccgcgtcct gtcatcggcg ctgatcggca tcttcagcgg catcatcctc tcgggcagcc
     1201 tctatgcgct cccggagttc ctgcgcaaca tcgatcccga accccacagc gccacgcgaa
     1261 ccggacaggt catgtgtgcc tatgcgctgg ccgccgcggc gatacggccc ctggtcctgc
     1321 cgctcgtcgc ccggatcggc cagcgcaaga ctattgcgtt cgcgctcgtc atgctggtgg
     1381 catcgatgct gctctttgtg cggctgctga cgaccggaac gcccgatgcc gcctatatcg
     1441 cgccgctcgt gctctacgcc ttctgcctgg cgccgctgtt gcccgccgtc ggcagcggta
     1501 ccgtcggccg ggtcgagcag gcgaggctgc tcgacggcgt ctcgctctac atgaccttcc
     1561 gccagttcgg cgcttcgatt ggcgtcgccg ttctcaccat cctcatcgag cagcgcgaga
     1621 cgctccattc ctcgcgcctc ttcgagcatc tccgcacggc gagcgtcacg accggggact
     1681 ggttgctgcg cgcgaccgcg atcatcgcgt cgcgggatgg cgagtcggcg atcgagtcga
     1741 gccacatggc gaccaagctt ctcgccgtgg ccggcagccg tcaggcggcg acgctcgcct
     1801 acgccgacgc cttcctgttc atggcgctga tcggcgtggc ggcgctctgt ctcgtgccga
     1861 tcatcccgcc gacgccgacg gagccgaaga aatgagcacc gctgccagcg acaaggcaac
     1921 ccccggccgt gctgccgagc cggtcggcgc gcccgcgtcg gcgcgcggcg atgccgccat
     1981 cgaggtccgg cgcgtggtcg aggccccgcc ccagccgcgc gtgcagcgaa gccgccgctc
     2041 gctggtgctc gtcgcggcgg cggcagcttt ggtcgctgcc gcgctctaca tctacgtgcc
     2101 gcgcttgttc ggggtcgcca ccgacgacgc ctatgtcgac gcgcatgtgg tgtcggtggt
     2161 gccgaaagtg tccgcctacg tcgtcgcgct gcacgtcgac gacaacacca acgtccgcgc
     2221 aggcgagctt ctcgtcgatc tggatccgcg cgatttcgag gtcgccgtcg cgagcgcggt
     2281 ggcagatctc gccagcgccg aggcgagcgc ggccaatatc gaggcgcaga cgacggagca
     2341 acaagcgctc atcgcgcaga gcgaggcgac gctcgagggc gaccgcgccg tggtcgcgtt
     2401 cgcccagcag cagctggcgc ggtacaccaa tctcgccaat agcggcgttg gcagcgtcga
     2461 gcggctgcag caggcgcagt ccgatatcgg agagcgcaag gcggtgctcg agcgcgaccg
     2521 tgcggcggtc gaggccgcgc gcacccatct ggcggtcctc gagacggcgc gccgccaggc
     2581 cgacgccaat atcgagcggc aacgggcggc cctggaccag gcgcggctca acctctccta
     2641 cacccaaata tcggcgcccg cggatggcac gatcgccaac aagactgtcc aggtcggcaa
     2701 cttcgtccag cccgggcaga tcctgttctc gatggtcccc gaccagctct atgtcacggc
     2761 caacttcaag gaaacccagc tcaccaatgt tcgcccgggc cagagcgccg tcgtccgagt
     2821 cgatgccttt cccggactgc gtctttcggg tcgcgtcgac agcatgcagc gcggcaccgg
     2881 tgcgcaattc gcgctgctgc cgcccgagaa tgcgaccggg aatttcgtca aggtcgtgca
     2941 gcgggtcccc gtcaagatca tcctggacga tcccggagag gcgctgcgct atatttcacc
     3001 cggcatgtcg gtcgaagcga cgatccattt tgccaagccg ccccggtggc tcgccttcct
     3061 cgagtgagcg gcttcggctc tgtcgcgccc ctcgcgcggg agcgcggtca ggcgatcgtc
     3121 tcgagcgcca gagcgatgcc ctggccgccg ccgatgcaca aggtgacgac gccgcggcgc
     3181 aagccgtcgc ggcgcatcga gtgaatgagg cgggtcgtca gaacggcgcc ggtggcgccg
     3241 atcggatggc catgggcaat ggcgccgccc tcgacattga cgatgtcggg cgagaggccg
     3301 agctcgcgcg tcacggcgat gacgatggcg gcgaaggcct cgttgatctc gatgcgctcg
     3361 atgtcggcga ttttccagcg cgcccgttcg agggcgtggc gcacggcggg gatcggaccg
     3421 aggccgaaca tgccgggctc gaccgcggcg atgccgtagg agacgagccg cgccaccgga
     3481 gcgagaccgc gtgcctcggc ccattgacga tccgccagga ccatagctga cgcgccgctg
     3541 ttgacgcccg gcgcgttgcc ggcggtgatc gtcccgtcct tgcgaaaggc cggcttcagc
     3601 gcggcgagcg cctcgagcgt ggtgttgggc cggttgtgct cgtccttgtc cacgatgatc
     3661 gggcctttgc ggcccggcac gtcgacggcc gcgatctcgg ccgtgaattt cccggcggct
     3721 tgcgccgcgg aaaagcgctg ctgcgagcgc aacgcccatt ggtcctgcgc ctcgcggccg
     3781 atctggaatt tctcggcgag atcctcggtg tgccaaccgg aatgctcgtc cgagaacgcg
     3841 tcgttcaagc cgtcgcgcag catgctgtca tagagctgcc cgtcgcccat gcggtagccc
     3901 cagcggccgc gcgcgatcag gtagggcgcg agatccatgt tctccatgcc gccggcgatg
     3961 gcgacgtcga cgctccccaa cagaatctcc tgcgccgcag aaacgatcgc ctgcgctccc
     4021 gacccgcaga cgcggttcac ggtcatggcc ggaacggaca ccggcagccc ggcatggatc
     4081 gccgcctggc gggcgggatt catcttgacg cccgcctgga tgacgttgcc gagcgccacc
     4141 gtggcgatgt cctccggccg gaggcgcgcg cgggcgagcg cggccttgat ggcg
//
LOCUS       DASYNS010000515         3531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4967521, whole genome shotgun sequence.
ACCESSION   DASYNS010000515 DASYNS010000000
VERSION     DASYNS010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4967521"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VGP50_15945"
     CDS             complement(<1..248)
                     /locus_tag="VGP50_15945"
                     /inference="COORDINATES: protein motif:HMM:NF012481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEV8028926.1"
                     /translation="MDQPLTILVGTMTGTAQLVAEEVADALKARGVAAEVLPMDRLDA
                     GVFARSPAFLICSSTYGQGDVPDNAQALFADLERAKPD"
     gene            351..1253
                     /locus_tag="VGP50_15950"
     CDS             351..1253
                     /locus_tag="VGP50_15950"
                     /inference="COORDINATES: protein motif:HMM:NF006015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV8028927.1"
                     /translation="MVTSEGADAGRENGEAAYLRLLGERIREARARRGMTRKLLARDS
                     SVSERYLAQLEAGQGNISIALLRQVAQAMGLPLADLVRDEPDRPVELTLLIQTLGRLS
                     PKELHEARRLLADAFGAAVESERRHRIALIGLRGAGKSTLGMLLARDLGVPFVELDRE
                     IERDCGTQLAEVFDLYGQAAYRRYERRALEAVIERHDRAVIATGGSIVSEPATFDLLL
                     SACYTVWLTARPEEHMSRVMAQGDYRPMAGNAEAMEDLRQILGGREALYRKADAVVDT
                     AGKTVEQSLGELKAATNPSPVTGG"
     gene            complement(1240..3444)
                     /locus_tag="VGP50_15955"
     CDS             complement(1240..3444)
                     /locus_tag="VGP50_15955"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV8028928.1"
                     /translation="MAEEAAGRLPQAEGHYRQALEIDPAMPSAHHRLGLLARRAGRHE
                     LARSFIVRAIELEPAKAEYHVDLGTVLSALKRFDDAERCYREALRFDPEDAQAHNGLG
                     NALGHRGRFEEAAEHYRATLRLKPDSPEPCFNLAQVLYRSGRFEEAEAACRDALRLKP
                     NYPEAYNHRGNIRSALGQFTAAEQCYREALRLNPTYVDAFRNLGDLLGNLRRFEQAVD
                     CYREALILDPDFPGTWNNLALALTSLGRAEEADACYSHALRLKPDFLAARLNRCVDRL
                     PLIYRDEAEIERVRADYAAELEAAWRVDLAASPPGAVGATGGKPPFLLAYQGRSDRDL
                     QARYGDFVARVMASLFPAWTSPPEVAPPGPGEPVRVGILSAYFYNHSNWKIPIKGWMS
                     ALDKSRFQLFAYSVGTWADDETAVAKALAHRYVEGPMLLERWAETIRGDRLHVLLIPG
                     IGMDPMTERLAGLRLAPVQATSWGHPDTSGLPTVDDYLSSDLMEPPDAQAHYTEHLVR
                     LPNLSIWYEPPALTAAALSRSDMGVPQEAVVYWCCQSLYKYLPRFDGIFARIAAAVPD
                     ARFLFIEYPHGQRVNAIFRERLAAVFAQAGLDAERYCQFLPSMPLARFTAISRLADIF
                     LDSLGWSGCNSTLEALSQDLPVVTMAGDLMRGRHSAAILAMLGLGELVAEGPDAFVDL
                     AVGLGRDPVRRRALAQHIACRKHRLYRDRAAIDGLAAYLSSAASRTRPITLP"
BASE COUNT          583 a         1138 c         1269 g          541 t
ORIGIN      
        1 aaatcgggct tggcgcgctc gagatcggcg aacagcgcct gcgcgttgtc ggggacgtcg
       61 ccctggccat aggtcgagct gcagatcaga aaggcagggc tgcgggcaaa gacgccggcg
      121 tcgaggcggt ccatcggcag cacctctgcc gcgacgccgc gcgccttgag ggcgtccgcc
      181 acttcctcgg ccacgagctg cgcggtgccg gtcatggtcc cgaccaggat ggtcaagggt
      241 tgatccaacg cgggaacctc cttccggcct ggacggcccg gtggaagaga ctataatgca
      301 tgttatttcc taggcaagca ttatagtgcc tatcggcgac ggagctgcgg atggtgacca
      361 gcgagggggc agacgccggg cgggagaatg gcgaggccgc ctatctccgc ttgctgggcg
      421 agcgcatccg cgaggcgcgt gcgcgccgcg ggatgacgcg gaagctcctg gcgcgcgatt
      481 ccagcgtttc cgagcgctat ctggcgcagc tcgaggcggg gcaaggcaac atctcgatcg
      541 cgctcttgcg ccaggtcgcg caggccatgg ggttgccgct cgccgatctc gtgcgcgacg
      601 agccggaccg gccggtcgag ctcacgctcc tcatccagac cctcggccgg ctttcgccca
      661 aggagctgca tgaggcgcgc cgcctcctcg ccgacgcctt cggcgccgcg gtcgagagcg
      721 agcggcgcca ccgcatcgcc ctcatcgggc tgcgcggcgc cgggaaatcg accctgggaa
      781 tgctcctcgc ccgcgacttg ggcgtgcctt tcgtcgagct cgaccgcgaa atcgagcgcg
      841 attgcgggac acagctcgcc gaggtcttcg atctctatgg ccaggcggcc tatcgccgct
      901 acgagcgccg cgcgctcgag gcggtgatcg agcgccatga ccgcgccgtc atcgccaccg
      961 gcggcagcat cgtctccgag cccgccacct tcgatcttct cctctccgcc tgttacacgg
     1021 tctggctcac ggcgcgaccc gaggagcata tgagccgcgt catggcgcaa ggcgactacc
     1081 gccccatggc cggcaatgcg gaggcgatgg aggatttgcg ccagatcctc ggaggacgcg
     1141 aggcgctcta ccgcaaagcc gatgcggtgg tggacaccgc cggcaagacc gtggagcaga
     1201 gtctcgggga gctcaaagcg gcgacgaatc cctctcccgt tacgggaggg tgatgggtcg
     1261 tgttcttgaa gcagcagagc tgagatacgc cgcgaggccg tcgatcgcgg cccggtcgcg
     1321 gtagaggcga tgcttgcggc aggcgatgtg ctgggcgagc gcgcggcgcc gcacagggtc
     1381 gcggccgaga cccacggcga ggtcgacgaa cgcatcgggc ccctcggcga cgagctcgcc
     1441 gaggcccagc atggcgagga tggcagcgga atggcggccg cgcatgagat cgccggccat
     1501 ggtcacgacc ggcagatcct gagacaaggc ttcgagcgtc gagttgcagc cggaccagcc
     1561 gagactgtcg aggaagatgt cggcgagccg gctgattgcc gtgaagcgcg cgagcggcat
     1621 cgagggcagg aattggcaat agcgctcggc atcgagcccg gcctgggcaa aaaccgcggc
     1681 gaggcgctcg cggaagatcg cgttcacgcg ctgtccatgc ggatactcga tgaagaggaa
     1741 gcgggcgtcg ggcacggccg cggcgatgcg cgcgaagatg ccgtcgaagc gcggcaggta
     1801 tttgtagagc gactggcagc accagtagac caccgcctct tgcggcacgc ccatatcgct
     1861 gcgcgacagc gccgcggcgg tgagtgccgg cggctcgtac cagatcgaga gattcggcaa
     1921 tcgcacgaga tgctcggtgt aatgggcctg ggcgtccggc ggctccatca agtcgctgct
     1981 gagatagtcg tcgacggtcg gcaggccgga ggtgtccgga tgtccccagg aggttgcctg
     2041 cacgggcgcg agccgcaagc ccgcgagccg ttccgtcatc ggatccatgc cgatgcccgg
     2101 gatcagcaac acatggagcc ggtcgccgcg gatcgtctcg gcccagcgct cgagcaacat
     2161 cggtccttcg acgtatcggt gagcgagtgc tttcgccacc gccgtctcat cgtccgccca
     2221 agtgccgacg ctataggcga agagctggaa gcggctcttg tcgagcgcgg acatccagcc
     2281 cttgatcggg atcttccagt tggagtgatt gtagaaatag gccgagagga tgccgacgcg
     2341 gacgggctcg cccggcccgg gcggcgcgac ctcggggggc gacgtccagg ccggaaaaag
     2401 gctcgccatc acccgcgcga cgaaatcgcc atagcgcgcc tggaggtcgc ggtcggagcg
     2461 cccctgatag gccaggagga aaggcggctt gccgccggtg gcaccgacgg cgcccggcgg
     2521 cgacgcggcg agatcgacac gccaggcggc ctcgagttcc gccgcataat cggcgcgcac
     2581 gcgctcgatc tcggcttcgt cgcggtagat gagcggcagg cggtcgacgc agcggttgag
     2641 gcgcgccgcg agaaaatccg gcttcagacg aagcgcatgg ctgtagcagg catccgcctc
     2701 ctcggcgcgg ccgaggcttg tcagggcgag cgcgaggttg ttccaggtgc cggggaaatc
     2761 ggggtcgagg atcagcgcct cgcgatagca gtcgacggcc tgttcgaagc ggcggaggtt
     2821 gccgaggaga tcgccgagat tgcgaaaggc atcgacatag gtcgggttca gccgaagcgc
     2881 ctcgcggtag cattgctcgg ccgccgtgaa ttgcccgagc gcgctccgga tattgccgcg
     2941 gtgattgtag gcttcggggt agttgggctt gaggcgcagc gcgtcgcggc acgcggcctc
     3001 cgcctcctcg aagcggccgc tgcggtagag cacttgcgcc agattgaaac agggctcggg
     3061 cgagtcgggt ttgaggcgca gcgtggcgcg gtaatgctct gccgcctcct cgaagcggcc
     3121 gcgatggccg agcgcgttgc ccaggccgtt atgcgcctgc gcatcctccg ggtcgaagcg
     3181 cagcgcctcg cgatagcagc gctcggcgtc gtcgaagcgc ttcaaggcgc tcagcaccgt
     3241 gccgagatcc acatgatact cggccttcgc cggctcgagc tcgatggccc ggacgatgaa
     3301 gctccgcgcc agctcgtgcc gccccgcgcg gcgggcgagc aggcccagcc ggtgatgggc
     3361 ggagggcatg gccggatcga tttcgagggc ctggcggtag tgcccttccg cctgcggcag
     3421 gcgcccggcg gcctcctcgg ccatccccaa tcgcaaaaat tctgcgatag tcatgacttt
     3481 tcaagtatcg cctattttcc ggggctgcgc cagccattca gccctggctg c
//
LOCUS       DASYNS010000516         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5275682, whole genome shotgun sequence.
ACCESSION   DASYNS010000516 DASYNS010000000
VERSION     DASYNS010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5275682"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="VGP50_15960"
     CDS             <1..1829
                     /locus_tag="VGP50_15960"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEV8028929.1"
                     /translation="VIAAMHGTALGGGLELALAAHFRIGTADAKFGLPEVNLGILPGA
                     GGTQRLPRLVGLPLALEMITSGNPIGARQARENGLIDEVASGDLRAGAIAFSERVLRD
                     RLPLRRVRDLQDKLKVPEADFFKTAKSALAKRRRGLEAPQACIDAVEAAATLPFDQGL
                     KRERELFAKLLGGEQSKAQRYFFFAEREAAKIPDVPSDTPTKTIRRAAVIGAGTMGGG
                     IAMNFANVGIPVTIIEAEQKALERGLGLVRKNYETSASRGGMAAEDVDRRMALIKGSL
                     SYDDVADADIVIEAIFENMALKKEIFGKLDRIAKPDAILATNTSTLDVDEIAASTKRP
                     ESVIGLHFFSPANVMRLLEIVRGAKTSKPVIASSMTLAKTIRKVPALVGVCYGFVGNR
                     MLHQRGREAERLLIEGALPHQVDKVLTDFGFPMGPFAMGDLAGLDVGWRVRQERGTKS
                     PIADALHEAGRLGQKTNAGYYHYEPGSRAPIPDPAVEHIIVDVSKQLGVERRTIPEPE
                     ILERLLYPMINEGAKILEEGIALRPSDIDVIWIYGYGWPIYRGGPMFYADQVGLKKIQ
                     DRLQDMAQRFNEPSLKPAKLLATLAAEGRSFADWKQPAKAAS"
     gene            1829..2128
                     /locus_tag="VGP50_15965"
     CDS             1829..2128
                     /locus_tag="VGP50_15965"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEV8028930.1"
                     /translation="MVDEIAEIEVKPGLEAEFEANVAKAAPLFKRAKGCNGLELHRSI
                     EKPSRYRLVVQWETVENHTVDFRGSNDFQEWRKLVGHCFAGTPVVEHTRRVLKGF"
     gene            complement(2130..2873)
                     /locus_tag="VGP50_15970"
     CDS             complement(2130..2873)
                     /locus_tag="VGP50_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV8028931.1"
                     /translation="MTAARFLRDPLAWSLGAFILLVLFMPASRPLFAALFPELERPLY
                     TQDSFLALALSHLALVAASSAAAAALGVAGAIFVTRRAGAEFRGLVETLVAMGQSFPP
                     VAVLAIAVPVIGFGAEPALIALTLYGILPVLENTLAGLGAVPATVRDAARGIGMGEGA
                     ILLGVELPLAAPVIVAGIRTSTIINVGTAAIASTVGARTLGSPIIIGLNAANIAYVVQ
                     GAILVGLLAVVLDLAFDRLVARTRRWRDP"
     gene            complement(2870..3796)
                     /locus_tag="VGP50_15975"
     CDS             complement(2870..3796)
                     /locus_tag="VGP50_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028932.1"
                     /translation="MIELERVTKRYGAMVAVDAVSLAIPTGAFCVLIGSSGAGKSTTL
                     RMINRLIAPSEGMIRIDGRDAASVKPEELRRRMGYVIQSIGLFPHWTVERNIGAVPAL
                     LGWPAARIGDRVGELLSLLKLDPEAVRRKYPHQLSIGQQQRIGIARALAADPDLLLMD
                     EPFGALDPITRDALQAEIARIHAASRKTIVFVTHDMDEALKLATVVAIMDRGRLVQAG
                     TPLELLTTPVNDFVRDFVGREDLGMRLLSVEKVAQRLRAGETAPGEPISAEASLRQAL
                     SQMIARGSDRIAVVDAAGRSRGVLHLADLVRQ"
BASE COUNT          693 a         1358 c         1342 g          534 t
ORIGIN      
        1 cggtgatcgc cgcgatgcac ggcacggcgc tgggcggcgg gctcgagctc gcgctcgccg
       61 cccacttccg catcggcacg gccgatgcca agttcggcct ccccgaggtc aacctcggca
      121 tcctccccgg cgccggcggc acgcaacggc tgccgcgcct cgtcggcctg ccgctcgccc
      181 tcgagatgat caccagcgga aacccgatcg gcgctcgcca ggcgcgtgag aacggcctca
      241 tcgacgaggt cgcgtcaggc gacctccgcg ccggcgcgat cgccttttcc gagcgagtcc
      301 tgcgcgaccg gctacctctg cggcgcgtgc gcgacctcca ggacaagctc aaagtgccgg
      361 aggccgattt cttcaagacg gcgaagagcg cgctggcaaa gcggcggcgc ggcctcgagg
      421 cgccgcaggc ctgcatcgac gccgtcgagg cggcggcgac gctgcccttc gaccagggcc
      481 tgaagcgcga gcgcgagctt ttcgccaagc tgctcggcgg cgagcagtcg aaggcgcagc
      541 gctatttctt cttcgccgag cgcgaggccg ccaagatccc ggacgtcccc agcgacacgc
      601 cgaccaaaac gatccgcaga gccgcggtga tcggcgccgg caccatgggc ggcggcatcg
      661 ccatgaactt cgccaatgtc ggcatccccg tgacgatcat cgaggcagag cagaaggcgc
      721 tcgagcgcgg cctcggcctg gtgcgcaaga actacgagac cagcgcgagc cgcggcggca
      781 tggccgcaga ggatgtcgac cggcgcatgg cgctcatcaa gggctcgctg agctatgacg
      841 acgtcgccga cgccgacatc gtcatcgagg cgatcttcga gaacatggcg ctgaagaaag
      901 agatcttcgg caagctcgac cgcatcgcca agccagatgc catccttgcg accaacacct
      961 cgacgctcga cgtcgacgag atcgctgcat ccacgaaacg gccggaaagc gtcatcggcc
     1021 tgcatttctt cagcccggcg aatgtgatgc ggctcctcga gatcgtgcgc ggcgccaaga
     1081 cctcgaagcc ggttatcgcc tcgtccatga cgctcgccaa gaccatccgc aaagtcccgg
     1141 cgctggtggg cgtctgctac ggcttcgtcg gcaatcgcat gctgcatcag cggggacgcg
     1201 aggccgagcg cctcctgatc gaaggcgcgc tgccgcacca ggtcgacaag gtgctgacgg
     1261 atttcggctt ccccatgggt cctttcgcca tgggcgatct cgccggtctc gatgtcggct
     1321 ggcgcgtgcg ccaggagcgc ggcaccaagt cgccgatcgc ggacgcgctc cacgaggccg
     1381 gccgcctcgg gcaaaagacc aatgcgggct actatcacta cgagcccgga agccgcgcgc
     1441 cgattcccga tcccgcggtc gagcacatca tcgtcgacgt ctcgaagcag ctcggcgtcg
     1501 agcgccgcac catccccgag ccggagatcc tggagcggct gctctacccg atgatcaacg
     1561 agggcgcgaa gatcctcgag gaaggaattg cgctccgtcc cagcgacatc gacgtgatct
     1621 ggatctacgg ctatggctgg ccgatctatc gcggcggccc gatgttctat gcggaccagg
     1681 tcgggctcaa gaagatccag gatcgccttc aggacatggc gcagcgtttc aacgagccgt
     1741 cgctcaagcc ggcaaagctg ctggcgacgc tcgccgccga aggccgcagc ttcgccgact
     1801 ggaagcagcc cgcgaaagca gcctcctgat ggtcgacgag atcgccgaga tcgaggtgaa
     1861 gcccggcctc gaggccgagt tcgaggccaa tgtcgccaag gcggcgccgc tcttcaagcg
     1921 tgccaagggc tgcaacggcc tcgagctcca ccgctcgatc gagaagccga gccgctatcg
     1981 gctggtcgtg caatgggaga cggtcgagaa ccacaccgtg gatttccgcg gctcgaacga
     2041 cttccaggaa tggcgcaagc tggtcggcca ttgcttcgcc gggacgcccg tggtcgagca
     2101 cacgcgccgg gtgctgaagg gcttctgagt cacgggtcgc gccagcgccg cgtccgcgcg
     2161 acgaggcggt cgaatgcaag gtcgagcacg accgcgagaa gcccgaccag gatcgcgccc
     2221 tgcacgacat acgcgatgtt ggcggcgttc aatccgatga tgatcggcga gccgagcgtc
     2281 cgcgcgccca cggtggacgc gatcgccgcc gtgccgacat taatgatggt cgacgtgcgg
     2341 attcccgcga cgatgacggg cgccgccagc ggcagctcga cgccgagcag gatcgcgccc
     2401 tcgcccatgc cgatgccgcg cgccgcgtcg cgcacggtcg ccggcaccgc gccaagcccc
     2461 gcgagcgtgt tctcgagcac gggcaggatg ccatataggg tcaaggcgat gagcgccggc
     2521 tcggcaccga agccgatcac cggcacggcg atcgccagca ccgccaccgg cgggaaggac
     2581 tggcccatgg ccaccagcgt ctcgacgagc ccgcggaact ccgcgccggc gcggcgcgtg
     2641 acgaagatgg cgccggcgac gcccagcgct gcggccgcag cgctcgatgc cgccaccaag
     2701 gcgagatgcg acagcgcgag cgcgagaaag ctgtcctgcg tgtagagggg gcgctcgagc
     2761 tcggggaaga gcgcggcgaa gagcggccgc gacgccggca tgaacagcac caggaggatg
     2821 aaagcgccca gcgaccaggc gagcgggtcg cgcaggaatc gggcggccgt cattgccgca
     2881 cgagatccgc cagatgcagc acgccgcggg accggcctgc ggcatcgacg accgcgatgc
     2941 ggtcgctgcc ccgcgcgatc atctgggaca gggcctggcg caagctcgcc tccgccgaga
     3001 tcggctcacc cggcgccgtc tcgcccgcgc gcaagcgctg cgccactttt tccaccgaca
     3061 agagccgcat gcccagatcc tcgcggccga cgaagtcgcg cacgaaatcg ttgaccggcg
     3121 tcgtcagcag ctcgagcggc gtccccgcct gcacgagacg gccgcgatcc atgatggcga
     3181 cgaccgtcgc gagcttgagc gcctcgtcca tgtcgtgggt gacgaagacg atggtcttgc
     3241 ggctcgccgc gtgtatgcgg gcgatctccg cttgcagcgc gtcccgcgtg atcgggtcga
     3301 gggcgccgaa aggctcgtcc atgagaagca ggtcgggatc ggcggccagc gcgcgggcga
     3361 tgccgatgcg ctgctgctgg ccgatcgaga gctgatgcgg gtatttgcgg cgcaccgcct
     3421 cgggatcgag cttcaggagc gagaggagct cgcccacgcg atcgccgatc cgcgccgctg
     3481 gccagccgag caatgccggc accgcgccga tgttgcgctc caccgtccaa tgcgggaaga
     3541 ggccgatcga ttggatcaca tagcccatgc gccgccgcag ctcctcgggc ttcacgctcg
     3601 cggcgtcccg gccgtcgatg cggatcatcc cttccgaggg cgcgatcagg cggttgatca
     3661 tgcggagcgt cgtcgacttg ccggcgccgg aagagccgat gagcacgcag aaggcgccgg
     3721 tcggaatggc gagcgatacc gcgtccacgg cgaccatcgc gccgtagcgc ttggtcacgc
     3781 gctcgagttc gatcatgcga ccgaccggct gaggcgcgca gcggcgctgc gcagcgacag
     3841 gtcgacgaca agcgccagca ggaccacggg cagcgcgccg agaaggacca ggtcgagcgc
     3901 atcggcgaag aggccttcga acatgat
//
LOCUS       DASYNS010000517        14904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2625580, whole genome shotgun sequence.
ACCESSION   DASYNS010000517 DASYNS010000000
VERSION     DASYNS010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 14904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14904
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2625580"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="VGP50_15980"
     CDS             <1..99
                     /locus_tag="VGP50_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8028933.1"
                     /translation="LRRKLEPDPQKPTMIKTVRGTGYIFTPQVEVS"
     gene            303..1283
                     /locus_tag="VGP50_15985"
     CDS             303..1283
                     /locus_tag="VGP50_15985"
                     /inference="COORDINATES: protein motif:HMM:NF017807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV8028934.1"
                     /translation="MTVGASLPENRDGGRRTTLLPYLLLSLAALIWAGNWVVGRGIRD
                     TVPPVALAFWRWAIAALILAPIAVPRLRGRLPLLRRHWRVLFVLSAGGIALFQCMIYA
                     GLRYTNAVNAMLMNSAMPLVMMLVAWLIDRQRVTPRQVAGMVVSFCGILVIVNRGDLA
                     TLSNFSFNPGDLIILLGMPAWGVYSVLLRRRPLELDSLTFVFVLAACGALLLAPAYGL
                     ESRFLRAPLLTWSAVGAVLYVALFASIAAYLCWNRGVDMVGPTNAGFTQHLVPAFGTV
                     LALIFLGEEVHLFHAIGIATILLGVWLATRKAAVAALDLTSPMRGGERRV"
     gene            complement(1246..2184)
                     /locus_tag="VGP50_15990"
     CDS             complement(1246..2184)
                     /locus_tag="VGP50_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEV8028935.1"
                     /translation="MTMTRLEQEPDARSHTLAELMGLAAAMESEAILRYGQLAAEMSR
                     RGDHGLAAMFQVLLEEERAHLAGIERWSRATTGDSPPAARRPWQLPPEIARSWEEAVR
                     SALLTPYRALSIAVLNEDRGFAFYTYVAARAEDTAARAAAERLAAEELNHAALLRRER
                     RRAWRSEQRGQTPAERPITPDATDFARRAWRIETDAAQRHARLAERLAALKHGEDASA
                     LHAIAEEERQAARIAGSGAAGHASVAAAEEPAPEANRAALLRASLAESERLYDIYADL
                     ANQAGTETVLLAAQDGARRAVRHIATLVVRLHASGS"
     gene            2220..4529
                     /locus_tag="VGP50_15995"
     CDS             2220..4529
                     /locus_tag="VGP50_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEV8028936.1"
                     /translation="MSSFAMICVNDRQKRPEHQSASRGTPRSTETMHRLSTSLASGLC
                     IVFLALAAIAPGEASAKGRLGEFLPRLTPGDIDPAADRFGDIAGDPPLAPLLKDGQLV
                     GYAFLNADVVDATGYSGKPINIVIGLDRDGRITGAKLVEHHEPIVLVGIAESKVLHFI
                     DGYIGRNVLESGAVERTPVDIVSGATVSMMVIGDSIMRSAIKVARANGIGASPGAAVA
                     AAAPQRQVAPAMGERADWRTLLGDGSVRRLALSNKDINDAFSASGNAAAIAHSETGAP
                     EAPYVDLYVALASIPTVGRSLLGDADYATLAQRLEPGQQAILIAGDGPYSFRGSGYVR
                     GGIFDRIEVIQGDSSLRFHDRDYKRVGALAPDDAPDFPEIGLFVTPEGAGLDPAAPWR
                     LQLLVQRRVGAMDKAFITVETTYTTPDKYLIAVAPPPAAARQAAAAEVASDAAEDAAP
                     LWQRVWQGKVVEIGILLVAIGVLTGIFFFQDVLVKRPVLYERVRLSYLAFTLLWLGWY
                     EHAQLSVVNVFTFITAVRGNFRWDYFLLDPLVFILWCSAAAALLFWGRGAYCGWLCPF
                     GALQEWMNRLARLCRVKQLSVPFRLHQRLWPIKYMIFLALFGISLSSLALAEEGAEIE
                     PFKTAIVLHFFRDWWFVLLALALLAPGLFIERFYCRYMCPLGAALAIPARMRMFDWLA
                     RYRECGSPCQRCAGDCPVQAIHPEGQINPNECIQCMNCQVLYHHDQKCPVMIQKRLKR
                     ERRLALSSPSMRPGASAAGTSLASVDSDD"
     gene            4573..6516
                     /gene="nosZ"
                     /locus_tag="VGP50_16000"
     CDS             4573..6516
                     /gene="nosZ"
                     /locus_tag="VGP50_16000"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-dependent nitrous-oxide reductase"
                     /protein_id="HEV8028937.1"
                     /translation="MSDQEKAKDEKSGVSRRDLLGSTATVAAVAGIAGAAGGAGLTSL
                     SARAFAQEAQPRAKAQTAEVRPGDLDEYYGFSSSGQTGEIRIMGIPSMRELMRIPVFN
                     RDSATGWGLTNESRKILTEGLLPATRKFLETRGGSFVNGDLHHPHMSFTEGTYDGRYI
                     FVNDKANCRIARIRCDVMKCDKIIEIPNASDIHGLRPQKYPRTGYVFANSEHIIPMPN
                     DGSVLDEPAKYWSVFTAVDGDTMKVAWQVLVDGNLDNCDADYQGKYAFSTCYNSERGV
                     TQADMTSAERDWVTIFDIKRIEEAIKKGDYKKMEGVPVVDGRHGSRYTRYIPVANNPH
                     GCNMAPDGIHLALAGKLSPTVTLIDVRLFDQLFNDKIQPRDAIVAEPELGLGPLHTAF
                     DGRGNAYTTVFIDSQVVKWNMDKAKRAFKGEKVDPIVQKLDVHYQPGHNHSSMGETKE
                     ADGKWLMSLNKFSKDRFLPVGPLHPENDQLIDISGDEMKIVHDGPSFAEPHDCTIVHR
                     SKVNPIIVWKRDDPMWEDIRQQAAKDKVKLEEAANVIRDGNKVRVYMYSMAPAYSLDK
                     FTVKQGDEVTVTITNMDDVEDVTHGFTIVRYGVMMEISPQQTASVTFIADRAGVHWYY
                     CQWFCHALHMEMAGRMIVEPHAA"
     gene            6513..7796
                     /locus_tag="VGP50_16005"
     CDS             6513..7796
                     /locus_tag="VGP50_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HEV8028938.1"
                     /translation="MSGRRPLPGRAGAAFLAGLLSLASAAAAAAALEVAAGSDRLRQA
                     VATSRPGDVLALGPGVHDGPIRIDHPLTLEGAAGAIVDGHGVASTIVVAAPDVVIRGI
                     AVRGSGIDDAMDAGVFLDQSAARALVERDHIEGNLVGVYVHGAAGAVVRDNTIVGRTD
                     LRLNERGNGVYVWNAPGAQVIGNDISGGRDGIFTNTSRQNVFRGNRLHGVRFAVHYMY
                     TNDSEVSGNVSQGNHIGYALMYSHGLDVRDNLSEDDREHGLLLNYTNESVITGNTVRR
                     SDKCVFIYNANKNRIERNWFEGCAIGVHFTAGSERNSIAGNAFVRNRTQVMYVGTRAL
                     DWSVNGRGNYWSDDPAFDLNGDGIADTAYRPNDIIDKVEWSYPTAKLLMNSPAVQVIR
                     WAQSQFPALHPGGVIDSAPLMKPAFVPPADGAEEH"
     gene            7796..8710
                     /locus_tag="VGP50_16010"
     CDS             7796..8710
                     /locus_tag="VGP50_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8028939.1"
                     /translation="MGTPTIGITGVSKRYGEQHAVRAVDLALDAGECVALVGHNGAGK
                     STLIKMILGLTRPDAGRIAVLGEDPCGRHTAKLRARLGFLPETVAFPATMTGREVIDF
                     YARLKHQPLARNTALLERVGLAEAARRRVGTYSKGMRQRLGLAQALLGEPSVLLLDEP
                     TSGLDPASRQSFYALIGELRDGGATVLLSSHALGEIEEHTDRIAVMSRGRLIACGTLA
                     ELRGLASLPVRIRVQFAEPAAAAVLIMQGGGAWTRLDARRLELACANDAKLAMLRQIS
                     SCAGIADIDVVPPTLDEIYAHMMREAAE"
     gene            8707..9537
                     /locus_tag="VGP50_16015"
     CDS             8707..9537
                     /locus_tag="VGP50_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV8028940.1"
                     /translation="MTAVLVIAGAEIRAARRNRWVLATTLLLAALALTLAFLGSAPTG
                     TVGAGALEVTVVSLSSLTIFLVPLIALLLSYDAIVGEAERGTLLLLLSYPMERWQIVL
                     GKFLGHLAVLAFATALGYGAAGLVLGWSEAAPDAESWVAFADMIGSSIVLGAAFLALG
                     YLVSVAVRDRGFAAGLAIGLWLALVLLYDMALLGILVADQGKSITAGLFNDLLLLDPA
                     DIYRLFNLSGFAKVSLFSGMAGLSGEAQFGRAALLAALAAWVVVPLTLAALLFRRKQF
                     "
     gene            9537..10070
                     /locus_tag="VGP50_16020"
     CDS             9537..10070
                     /locus_tag="VGP50_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEV8028941.1"
                     /translation="MRRSSFALLMSAVLAVSLVACDKEKVAARPAPLEPTAESVAHFC
                     DMAVLDHPGPKGQIFLEGRAEPVWFASVRDTFAFTLLPEEPKDIAAIYVNDMGKAKEW
                     QRPEAGTWVEARAAWYVLGSDYGAGMGGREAVPFGAEADARGFAEAHGGRLARFAEVP
                     RDYVLDSETAEGHEPTH"
     gene            10091..11098
                     /locus_tag="VGP50_16025"
     CDS             10091..11098
                     /locus_tag="VGP50_16025"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV8028942.1"
                     /translation="MRPAITRRRFIRITGIAAGLGIAPWAGALHAAAPSPLLHRWSGI
                     ALGADASLQIYHSDAQVAAALIDAALAEVKRLEKIFSLYDATSALRCLNRDRTLADPP
                     QELVELLAACERFSRATGGAFDVTVQPLWDLYAAHFATADADPAGPPAAAVHAALARV
                     DHAALDVDPALIRFAQSNMGVTLNGIAQGDITDRVAALLRAHGIGHTLVDMGETRALD
                     GHPTGRPWTVGIRDPRVEGGILRILPLEDQAIATSGGYGTQFDDAGRFNHIFDPITGG
                     CATRYLSVSVVAPIATTADALSTALSVMPLDRAEAALRDAGATAAWFVLADGSIVERR
                     A"
     gene            complement(11126..11764)
                     /gene="grxB"
                     /locus_tag="VGP50_16030"
     CDS             complement(11126..11764)
                     /gene="grxB"
                     /locus_tag="VGP50_16030"
                     /inference="COORDINATES: protein motif:HMM:NF007702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HEV8028943.1"
                     /translation="MKLYMFEHCSLCFRVRMAAALKGLHLQEVVVLEDDTDTMVGLVG
                     KRVVPIVVTDEGKPMLESMDMVRYIDRRGEPILTGPERREIDAWAGEFAAKTAPLTWP
                     RYPLLGLPEFATVAALDHYMLRKRKAIGDFVELRANTRQYVKALMPDLDALDRMIESP
                     LAVNGTISYDDIRVLPLLRSAAVVAGLRFPRKVRDYFESMMSRLGHRPLPAV"
     gene            complement(11761..12117)
                     /locus_tag="VGP50_16035"
     CDS             complement(11761..12117)
                     /locus_tag="VGP50_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013641216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV8028944.1"
                     /translation="MKAPQAVASLGALAHETRLEVFRLLVQRGPDGMSAGTIAQRVKL
                     PPSSLTFHLQQLARAGLITQRRLSRQLFYAADFTAMNGLIGYLTENCCRGAAAGASVC
                     QPAKAGPAVAQRKRSA"
     gene            complement(12345..12941)
                     /locus_tag="VGP50_16040"
     CDS             complement(12345..12941)
                     /locus_tag="VGP50_16040"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8028945.1"
                     /translation="MTRASPLKPAVDPRIERSRQVILGAALEELGEIGYGGFAIESVA
                     ARAGVGKSTIYRHWPDKLALIADAFRTFHEAAGPDIARGSPREKLLAILRHVAEVVVS
                     STFSACIPALVDAAERDQDLRKFHLHFQREARRPLINVIADGVADGSFPSHVVPELAA
                     FALLGAIFFCRLMTNKPLDPERVGELIETVIGASAQRA"
     gene            complement(12938..13357)
                     /locus_tag="VGP50_16045"
     CDS             complement(12938..13357)
                     /locus_tag="VGP50_16045"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV8028946.1"
                     /translation="MPACFRYIVDDVDASIEFYTKRLGFKLEMHPAPPFAEISRGDMH
                     IYLTQPSRMGGGAAMPSGEEQTPGGWNRVHLVVDDLDAVIARLNGAGCRFRNEVVQGV
                     GGKQILLEDPSGNLVELFQPNTADYRAPGSGGAAPAS"
     gene            complement(13481..14794)
                     /locus_tag="VGP50_16050"
     CDS             complement(13481..14794)
                     /locus_tag="VGP50_16050"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV8028947.1"
                     /translation="MDDRHPIVVVGGGAGGLELATRAGNTLGKSGQAQIVLVEKARTH
                     LWKPLLHAVAAGSMDPAEHELNYLAQAHWHHFQYRLGEMIGLDRKKKEVRLAPTFDEE
                     GRQITPPRAIGYGTLVIAIGSVTNDFGTAGAARFAVPLDTPEQAVRFNHRLVNACIRA
                     DAQAEPVRPGQLHVAVIGAGATGTELAAELHRTARQVVAFGLDRIDPEKDLRIILIEA
                     AERILPALPERVADATVALLRTLGVEVRAGARVSEVRADGVVLEGGAFIPSELVVWCA
                     GVKAPEVLRDLDGLEVNRLNQLVVTPLLQTTKDPDIFAMGDCASCPREGYPAPVPPRA
                     QAAHQQASHLLRQLRRRLAGQPLEPFVYRDFGSLVSLGDYSTVGNLMGFLIGKNLFIE
                     GYFARLMYRSLYKMHEYALQGGGRTFLGALARQLSRGAEPEVKLH"
BASE COUNT         2391 a         5215 c         4817 g         2481 t
ORIGIN      
        1 ctccggcgca agctcgagcc cgatccgcag aagccgacga tgatcaagac cgtgcgcggc
       61 acgggctata tcttcacgcc gcaggtcgag gtcagctagc gcctcccggt taccccagaa
      121 tcattcttgc ttcgtcgtcg ccgcgaagaa tacgggggcg ttcgctgcgt cctggagccc
      181 cgccgcgggg gttcgcggcc acgggcaacg gggagctgac gtcgagagag ggcctcggcg
      241 catgtcagcc ttcccgcgcc gtccattgac gcgcggccgg cccgcccgca ctctcgccgc
      301 cgatgaccgt cggcgcgtcc cttccagaga accgagacgg cggccggcgg acgacgcttc
      361 tgccttattt actgctgtcg ctcgccgccc tcatctgggc gggcaactgg gtggtgggcc
      421 gcggcatccg cgacaccgtg cctcccgtcg ctcttgcctt ctggcgctgg gcgatcgcgg
      481 ccctcatcct cgcacccatc gccgtgccgc ggctcagagg ccgcttgccg ctgctgcgcc
      541 ggcactggcg cgtgctcttc gtgctttccg ccggcggcat cgcactcttc cagtgcatga
      601 tctacgccgg cctgcgctac accaacgcgg tcaacgccat gctgatgaac tcggcgatgc
      661 cgctcgtgat gatgctcgtc gcctggctca tcgaccggca aagggtgacg ccgcgccagg
      721 tcgccggcat ggtcgtctcc ttctgcggca tcctcgtcat cgtcaatcgc ggcgatcttg
      781 cgacgctaag caatttcagc ttcaaccccg gcgatctcat catccttttg ggcatgccgg
      841 cctggggcgt ctattccgtg ctgctgcgcc gccggccgct cgagctcgat tcgctgacct
      901 tcgtcttcgt gcttgccgct tgcggcgcgc tgctgctggc gcccgcctat ggtctcgaaa
      961 gccgattcct gcgagcgccg cttcttacct ggagcgcggt cggcgccgtc ctttatgtcg
     1021 cgctcttcgc ctcgatcgcc gcctatctat gctggaatcg cggtgtcgac atggtcggcc
     1081 ccaccaatgc cggcttcacc cagcatctgg tcccggcctt cggcaccgtg ctcgccctga
     1141 ttttcctcgg cgaggaggtg catctcttcc acgccatcgg catcgccacg attcttttgg
     1201 gcgtctggct tgcgacccgc aaagccgccg tcgccgctct tgatctcacg agcccgatgc
     1261 gtggaggcga acgacgagtg tagcgatgtg gcgcacggcg cgcctggcac catcctgggc
     1321 ggcgagcagc acggtctcgg tgccggcctg gttggcaaga tcggcataga tgtcgtagag
     1381 ccgttcgctt tcggcgaggc tcgcccgcaa caaggccgcg cggttcgcct cgggcgccgg
     1441 ctcctcggcg gccgccacgc tcgcgtgccc cgctgcaccg ctcccggcta tccgtgccgc
     1501 ctgtcgctct tcttcggcga tcgcgtgtag cgccgacgca tcctcgccat gcttcaacgc
     1561 ggcgagacgc tcggcgaggc gcgcatgtct ttgcgccgcg tccgtctcga tccgccacgc
     1621 gcgacgggcg aagtctgtcg catcgggagt gatcggccgc tcggccggcg tctggccgcg
     1681 ctgctcgctc cgccaggcgc gccgccgctc gcggcgcagc agcgccgcat ggttcagctc
     1741 ctctgcggcg aggcgctccg ccgcggcgcg ggccgccgtg tcctctgcac gggcggcgac
     1801 ataggtgtag aaagcgaagc cacgatcttc gttgagcacg gcaatgctga gcgcgcggta
     1861 cggcgtcagc aacgcgctcc tcaccgcttc ctcccacgag cgggcgatct ccggcggcaa
     1921 ctgccacggc cgccgggcgg cgggcggaga gtcgccggtc gttgcgcgcg accagcgctc
     1981 gatgccggcc agatgggcgc gctcctcttc gagcagcacc tgaaacatgg ccgccaagcc
     2041 gtgatcgccg cgccgcgaca tttccgccgc gagctgtccg tagcgcagga tcgcctcgct
     2101 ctccatcgcc gccgccaagc ccatcagctc ggcgagggtg tgcgagcggg cgtccggctc
     2161 ctgctccaac cgcgtcatcg tcattgcatg ctcaagtagg ccacaaggct gccacatcga
     2221 tgagcagctt cgccatgatc tgcgtcaacg acaggcagaa gagaccggag catcaatccg
     2281 cgtcgcgcgg gacgccccgc tccacggaga ccatgcaccg cctctccaca tcattggcct
     2341 ccggcctgtg catcgtcttc ctcgccctcg cggcgatcgc accgggcgaa gcgtcggcaa
     2401 aaggccggct cggagaattc ctgccgcggc tcacgcccgg cgacatcgac ccggcggccg
     2461 accggttcgg cgatatcgcc ggcgacccgc ccctcgcgcc gctgctcaag gacggccagc
     2521 tcgtcggcta cgccttcctc aacgccgacg ttgtggatgc gacgggatat tccggcaagc
     2581 cgatcaacat cgtgatcggc ctcgaccggg atggccgcat caccggtgcg aaactggtcg
     2641 agcatcacga gcccatcgtc ctcgtcggga tcgccgaatc gaaggtcttg catttcatcg
     2701 acggctatat cggccggaat gtgctcgagt ccggcgccgt cgagcgcacg cccgtcgaca
     2761 tcgtcagcgg tgccaccgtc agcatgatgg tgatcggcga cagcatcatg cgctcggcga
     2821 tcaaggtcgc ccgcgccaac ggcatcggcg catcgcccgg cgcggccgtc gcggcggccg
     2881 cgccgcagcg acaggtcgcg ccggcgatgg gcgagcgcgc ggactggcgg acgctgctgg
     2941 gcgacgggtc ggttcgccgc ttggcgctca gcaacaagga catcaacgac gccttcagcg
     3001 ccagcggcaa cgccgccgcg atcgcccatt ccgagaccgg cgcgccagag gcgccttacg
     3061 tcgatctcta cgtcgcgctc gcaagcatcc cgacggtcgg gcgcagcctg ctcggcgacg
     3121 ccgattatgc gacgctcgcc cagcggctcg agccgggtca gcaggcgatc ctcatcgccg
     3181 gcgacgggcc ttattccttc cgtggatcgg gctatgtgcg cggcggcatc ttcgatcgca
     3241 tcgaggtgat ccagggcgac agcagcctcc gcttccacga ccgcgactac aagcgggtcg
     3301 gcgcgctggc acccgatgac gcaccggatt tccccgagat cggccttttc gtcacgcccg
     3361 aaggtgctgg gctcgatccg gcggcgcctt ggcggctgca gctcctggtg cagcgccggg
     3421 tcggcgcgat ggacaaggcc ttcatcaccg tcgagacgac ctataccacg cccgacaaat
     3481 acctgattgc cgtcgcgccg ccgcctgccg ccgcgcgaca agcggccgcg gcggaagtcg
     3541 cgtccgacgc cgcggaagac gcggcaccgc tctggcagcg cgtctggcaa ggcaaggtcg
     3601 tcgagatcgg cattctcctc gtcgccatcg gcgtgctcac cggcatcttc ttcttccagg
     3661 acgtgctggt gaagcggccc gtgctctacg agcgcgtccg gctgtcctat ctggcattca
     3721 cgctgctgtg gctcggctgg tacgagcacg cccagctctc cgtcgtgaac gtcttcacct
     3781 tcatcactgc ggtgcgcggc aatttccgct gggactactt cctgttggac ccgctggtgt
     3841 tcatcctctg gtgctcggcg gccgcggcgc tgctgttctg ggggcgcggc gcctattgcg
     3901 gctggctctg cccgttcggc gcgctccagg aatggatgaa ccgcctcgcg cgtctttgcc
     3961 gcgtgaagca gctcagcgtg ccgttccggc tgcatcaacg cctctggccg atcaaataca
     4021 tgatcttcct ggcgctcttc ggcatctctc tgagctcgct ggcgctggcc gaggaagggg
     4081 cggagatcga gcccttcaag acggcgatcg tcctccactt cttccgcgac tggtggttcg
     4141 tgctcctggc gctggcgctc ctcgctcccg gacttttcat cgagcgcttc tattgccgct
     4201 acatgtgtcc gctgggcgcg gcgctggcga tcccggcgcg catgcgcatg ttcgactggc
     4261 tcgcgcgcta tcgcgaatgc ggctcgcctt gccagcgctg cgccggcgac tgcccggtcc
     4321 aggcgattca tccggaaggc cagatcaatc ccaatgaatg catccagtgc atgaattgcc
     4381 aggtgctgta ccaccacgac cagaaatgcc cggtgatgat ccagaagcgc ttgaagcgtg
     4441 agaggcgcct ggcgctctcc tcgccgtcca tgcggcccgg cgcgtccgca gcgggcacgt
     4501 cattggcgtc cgtcgacagc gacgattgag cgtcgcgcga ccaaccctct tcatttggcc
     4561 aggagaacgc gcatgtcgga tcaggaaaaa gcgaaagacg agaaatcggg tgtcagtcgg
     4621 cgcgacctgc tcggcagtac cgcgacggtg gcagcggtgg ccggcatcgc cggcgcggcc
     4681 ggcggcgccg ggttgacgtc gctcagcgcg cgagcgtttg cccaggaggc gcagcctcgc
     4741 gcgaaagccc agaccgccga ggtcaggccc ggcgatctcg acgagtatta cggtttcagc
     4801 agcagcggcc agaccggcga gatccgcatc atgggcatcc cgtcgatgcg cgagctcatg
     4861 cgcattcccg tgttcaaccg cgacagcgcg acgggctggg ggctcaccaa cgagagccga
     4921 aagatcctca ccgagggcct gttgccggcc acgcgcaaat tcctcgagac gcggggcggc
     4981 agcttcgtca acggcgattt gcatcacccg cacatgtcgt tcaccgaggg gacctatgac
     5041 ggccgctaca tcttcgtgaa cgacaaagcc aattgccgga tcgcccgcat ccgctgcgac
     5101 gtgatgaagt gcgacaagat catcgagatt cccaacgcct cggacatcca cggcctgcgc
     5161 ccgcagaagt atccgcgcac aggctacgtc ttcgccaaca gcgagcacat cattccgatg
     5221 ccgaatgacg ggtccgtcct cgacgagccg gcgaagtact ggtcggtctt caccgccgtc
     5281 gacggcgaca ccatgaaggt cgcctggcag gtgctggtcg acggcaacct cgacaattgc
     5341 gatgccgatt atcagggcaa atacgccttc tcgacctgct acaactcgga acgcggcgtg
     5401 acgcaagccg acatgacctc ggccgagcgc gactgggtca cgatcttcga catcaagcgc
     5461 atcgaagaag cgatcaagaa gggcgattac aagaaaatgg agggcgtgcc ggtcgtcgac
     5521 ggccgccacg gctcgcgcta cacccgctac atccccgtcg ccaacaatcc gcatggctgc
     5581 aacatggcgc cggacgggat tcatctggcg ctcgccggca agctgtcgcc gaccgtcacc
     5641 ctcatcgacg tccggctgtt cgaccagctc ttcaacgaca agatccagcc gcgcgacgcg
     5701 atcgtcgccg agcccgagct cggcctcggg ccgctgcaca ccgccttcga tggccgtggc
     5761 aacgcctaca ccaccgtgtt catcgacagt caggtcgtga aatggaacat ggacaaggcg
     5821 aagcgcgcct tcaaaggcga gaaggtcgac cccatcgtcc agaagctcga cgtccattat
     5881 cagcccggcc acaaccacag ctccatgggc gagaccaagg aggcggacgg aaaatggctg
     5941 atgtcgctca acaaattctc gaaggaccgc ttcctgccgg tgggcccgct gcatcccgag
     6001 aacgatcagc tcatcgacat ctccggcgat gagatgaaaa tcgtccatga cggaccgagc
     6061 ttcgccgagc cgcatgactg caccatcgtc caccgctcca aggtgaaccc gatcatcgtc
     6121 tggaagcgcg acgacccgat gtgggaggac attcgccagc aggcggcaaa ggacaaggtc
     6181 aagctcgagg aggcggccaa tgtcatccgc gacggcaaca aggtgcgcgt ctacatgtat
     6241 tcgatggcgc ccgcctacag cctcgacaag ttcaccgtca agcaaggcga cgaggtgacg
     6301 gtcaccatca ccaacatgga cgacgtcgag gacgtgacgc atggcttcac catcgtgcgc
     6361 tacggcgtaa tgatggagat ctcgccgcag cagacggcgt cggtgacgtt catcgccgac
     6421 cgggccggcg tccattggta ttactgccag tggttctgtc atgcgctcca catggagatg
     6481 gcagggcgga tgatcgtcga gccgcacgcc gcatgagcgg ccggcggccg cttcccggca
     6541 gggccggcgc ggcgttcctc gccggcctgc tctcgctggc gagcgccgcc gccgcggcgg
     6601 cggcgctcga ggtcgccgcc ggcagcgacc ggctgcggca ggcggtcgcg acgtcgcgtc
     6661 ccggcgacgt gctcgcgctc gggccgggcg tgcatgacgg accgatccgc atcgaccatc
     6721 cgctgacgct cgagggcgcg gcgggcgcga tcgtcgacgg gcacggcgtc gccagcacca
     6781 tcgtggtggc agcacccgac gtggtgatcc gcgggatcgc cgtccgaggc tccggcatcg
     6841 acgacgcgat ggatgccggc gtctttctcg atcagagcgc ggcccgcgcc cttgtcgagc
     6901 gcgaccacat cgaaggcaac ctcgtcggcg tctatgtgca tggcgccgcc ggagccgtcg
     6961 tgcgcgacaa cacgatcgtc gggcgcacgg atctgcggct caacgagcgc ggcaacggcg
     7021 tctatgtgtg gaatgcgccc ggcgctcagg tgatcggcaa cgacatctcg ggcggccggg
     7081 acggcatctt caccaacacc agccggcaga acgtcttccg cggcaaccgc ctccacggcg
     7141 tccgcttcgc ggtccactac atgtacacca atgacagcga ggtgagcggc aacgtctccc
     7201 agggcaacca tatcggctac gccctcatgt actcgcatgg cctcgatgtt cgcgacaacc
     7261 tctccgagga cgaccgcgag catggtctgc tgctcaacta taccaacgag tccgtcatca
     7321 ccggcaacac cgtgcggcgc agcgacaaat gcgtcttcat ctacaacgcc aacaagaacc
     7381 ggatcgagcg caactggttc gagggctgcg cgatcggcgt tcacttcacg gcgggatcgg
     7441 agcgcaacag catcgccggc aatgccttcg tgcggaaccg tacgcaggtc atgtatgtcg
     7501 gcacgcgcgc gctcgactgg tccgtaaacg gccgcggcaa ctattggagc gacgacccgg
     7561 ccttcgacct gaacggcgac ggcatcgccg acacggccta ccggccgaac gacatcatcg
     7621 acaaggtcga atggtcctac ccgaccgcca aactgctgat gaacagcccg gccgttcaag
     7681 tgatccgctg ggcgcaatcg cagtttccgg cgctccaccc gggcggcgtc atcgacagcg
     7741 cgccgctcat gaaaccggcc ttcgtgccgc cggcggacgg cgcggaggag cactgatggg
     7801 aacgccgacg atcggcatca ccggcgtctc gaagcgctat ggcgagcagc acgccgtgcg
     7861 cgcggtggat ctcgccctcg atgccggcga atgcgtggcg ctcgtcggcc acaacggcgc
     7921 aggcaagagc acgctcatca agatgatcct cggcctcacc cgccccgatg ccggccggat
     7981 cgccgtgctc ggcgaagacc cctgcggacg gcacaccgcc aagctgcggg cgcgtctcgg
     8041 cttcctcccc gagaccgtcg cctttcccgc gacgatgacg ggccgcgagg tcatcgactt
     8101 ctacgcgcgc ctcaagcacc agccgctcgc gcgcaacacg gcgctgctcg agcgcgtcgg
     8161 cctcgccgaa gccgcccgcc gccgggtcgg cacttactcc aaaggcatgc gccagcgcct
     8221 gggcctcgct caggcgctcc ttggcgagcc gagcgttttg cttctcgacg agccgacgag
     8281 cggcctcgac ccggcatcgc ggcaaagctt ctacgccctc atcggcgaac tgcgcgacgg
     8341 cggcgcgacc gtgctgctgt cctcgcacgc gctcggcgag atcgaggaac acaccgatcg
     8401 tatcgccgtg atgagccggg gccggctgat cgcctgcggc acgctggccg agctgcgcgg
     8461 tctcgcgtcc ctgccggtgc ggatccgcgt ccagttcgcc gagccggccg ccgccgcggt
     8521 gctgatcatg cagggaggcg gcgcctggac gcggctcgac gcccgccgcc tcgagcttgc
     8581 ctgcgccaac gatgcgaagc tcgcgatgct gcgccagatc tcgagctgcg ccggcatcgc
     8641 cgacatcgac gtggtgccgc cgaccctcga cgagatctac gcccacatga tgcgggaggc
     8701 cgcggaatga ccgcggtcct agttatcgcc ggcgcggaga tccgcgcagc gcggcgcaat
     8761 cgctgggtcc tggcgacgac gcttcttctg gcggcgctcg ccctcaccct cgcctttctc
     8821 ggcagcgcgc ccaccggcac ggtcggcgcc ggcgcgctcg aagtgacggt ggtgagcctg
     8881 tcgagcctca cgatcttcct cgtcccgctg atcgcgctgc tcctctccta tgacgccatc
     8941 gtcggcgagg ccgagcgcgg cacgttgctg ctgctgctgt cctatccgat ggagcgctgg
     9001 cagatcgtgc ttggcaagtt cctcggccac ctcgccgtgc tcgccttcgc caccgcgctc
     9061 ggttacggcg ccgcgggcct cgtcctcggc tggagcgaag cggcgccgga cgcggagagc
     9121 tgggtggcct tcgccgacat gatcggctcg tccatcgtgc tgggcgccgc cttcctcgcc
     9181 ttgggatatc tcgtcagcgt cgccgtgcgc gaccgtggtt tcgccgccgg cctcgccatc
     9241 ggcctctggc tcgcgctcgt gctgctctac gacatggcgc tcctcgggat tctcgtcgcc
     9301 gatcagggca agagcatcac ggcagggctc ttcaacgatc tgctgctgct cgatccggcc
     9361 gatatctacc ggctcttcaa cctttcgggc tttgccaagg tcagcctctt ctcgggcatg
     9421 gccggactct caggagaggc gcagttcggc cgcgccgcct tgctggcggc gctcgccgcg
     9481 tgggtcgtcg tgccgctcac gctggccgcc ctcctcttca gaaggaagca gttctgatgc
     9541 gccgctcatc tttcgccttg ctgatgtcgg cggttctcgc cgtctcgctc gtcgcctgcg
     9601 acaaggaaaa ggtcgcggcg cggccggcgc cgctcgaacc gacggccgaa tcggtcgcgc
     9661 atttctgcga catggccgtg ctcgatcatc ccgggcccaa gggccagatc tttctcgagg
     9721 gccgggcgga gcccgtgtgg ttcgcgtcgg tccgcgacac cttcgccttc accttgctgc
     9781 ccgaggagcc gaaggacatc gccgcgatct acgtcaacga catgggcaag gccaaggaat
     9841 ggcagcgtcc ggaggccgga acctgggtcg aggcgcgtgc cgcctggtat gtcctcggca
     9901 gcgactatgg cgccggcatg ggcgggcgcg aggcagtgcc gtttggcgcg gaagccgatg
     9961 cgcgcggctt cgccgaggcc catggcggca ggctggcgcg cttcgccgag gtgccgcgcg
    10021 actatgtgct cgactccgag acggcggaag gccacgagcc gacgcattga ggcagcggcg
    10081 ggagacagtc atgagacccg cgatcacgcg gcggcgcttc atccgcatca ccggcatcgc
    10141 cgccggcctc ggcatcgcgc cctgggccgg cgccttgcat gcggcggcgc cgtcgccgct
    10201 cctccatcgc tggagcggca tcgctttggg cgcggacgcc tctttgcaga tctatcacag
    10261 cgacgcgcag gtcgcggccg cgctcatcga cgcggcgctt gccgaagtga agcgcctcga
    10321 gaagatcttc agcctctacg atgcgacctc ggcgctgcgt tgtctcaacc gcgaccgcac
    10381 gctcgccgat ccgccgcagg agctggtcga gctgctcgcg gcctgcgagc gcttctcgcg
    10441 cgcgaccggc ggcgccttcg acgtcacggt gcagccgctc tgggacctct atgccgctca
    10501 cttcgccact gccgacgccg atcccgccgg tccacccgcc gccgccgtac atgccgcgct
    10561 ggcgcgtgtc gaccacgccg cgctcgatgt cgatccggca ttgatccggt tcgcgcagag
    10621 caacatgggc gtgacgctca acggcatcgc gcaaggcgac atcaccgacc gcgtcgcggc
    10681 gctgctgcgc gcccacggca tcggccatac cctcgtcgac atgggcgaga cccgcgcgct
    10741 cgacggtcat cccacggggc ggccctggac ggtcgggatc agggatccgc gcgtggaagg
    10801 cggtatcctg cgcatcctgc cgctcgagga ccaggcgatc gccacctcgg gcggctacgg
    10861 cacgcaattc gacgacgccg gtcgcttcaa ccacattttc gatcccatca ccgggggctg
    10921 cgccacccgc tatctcagcg tctccgtggt cgcgcccata gcgaccaccg ccgacgcgct
    10981 ctcgaccgcg ctctcggtca tgccgctgga tcgcgcagaa gcggcccttc gcgacgccgg
    11041 cgccacggcc gcatggttcg tcctggcgga cggcagcatc gtcgagcgcc gcgcctgaga
    11101 cggtccggcc cgatcgcgtc ggtcgtcaga ctgcaggcaa cggccgatgg ccgagccggc
    11161 tcatcatcga ttcgaaataa tcgcggacct tgcgcggaaa gcggagcccc gccaccaccg
    11221 ccgccgaccg cagcagaggc aggacgcgaa tatcgtcgta agaaatggta ccgttgaccg
    11281 cgagcgggct ttcgatcatt cgatcgagtg catcgaggtc gggcatgaga gccttgacgt
    11341 attgccgcgt attggcccgc agctcgacga agtcgccaat ggctttgcgt ttacgcagca
    11401 tgtagtggtc gagcgccgcg accgtcgcga attccggcag gccgagcagc ggataccgcg
    11461 gccaggtgag cggcgcggtc ttggccgcaa attcgccggc ccacgcatcg atttcgcgtc
    11521 gctccggccc ggtcaggatc ggctcgccgc ggcggtcgat gtagcggacc atatccatgc
    11581 tctcgagcat gggcttgccc tcatccgtca ccacgatcgg aacgacccgc ttgccgacga
    11641 ggccgaccat ggtgtcggtg tcgtcctcga gcaccacgac ctcctgcaga tgcagtccct
    11701 tgagcgccgc ggccatgcgc acgcggaagc acagcgagca atgctcgaac atatagagct
    11761 tcatgccgac ctctttcttt gcgcaacggc cgggcccgcc ttcgctggtt ggcaaacgga
    11821 cgcgcccgcg gcagcgccgc ggcaacagtt ctcggtgagg tagccgatca ggccattcat
    11881 tgccgtgaag tcggccgcgt agaacagctg tcggctgagc cgccgctgtg tgatgaggcc
    11941 ggcgcgcgcc agttgttgca gatggaaggt aagcgatgac ggcggcagct tgactcgctg
    12001 cgctatcgtg ccggcggaca tcccgtccgg gccccgctgc accaacaggc ggaatacctc
    12061 gagccgggtt tcatgggcga gcgctcccag cgaagcgacg gcctgcggcg ccttcatcac
    12121 acgcctccat atttcgacga ctatcgtaca atacaggcgc ggccgtcctg ctgtcaacga
    12181 tattccgcgt aatatcgaaa tgtttattcg gtcatccggc tccacaacca caagggtcaa
    12241 gcggcccgag attgagctct cgcgaggcga cgtccacggc gtggttcgtc tttgcggatg
    12301 gctgcatcgt cgagcaccgc gtctgacgcg cgccgatcgc ctcgtcacgc tcgctgcgct
    12361 gacgctccga tcacggtttc gatgagttcg cccacccttt ccggatcgag cggcttgttc
    12421 gtcatcaatc ggcagaagaa tatcgcgccg agaagcgcaa acgccgcgag ctccggcacg
    12481 acatgcgacg gaaagctgcc gtcggcaacg ccgtcggcga tcacgttgat caagggacgt
    12541 cgtgcctcgc gctggaagtg gagatgaaac ttcctcaggt cctggtcgcg ctccgccgca
    12601 tcgacgagcg ccgggataca ggcggagaac gtggagctga cgacgacctc ggcgacatgt
    12661 cgcaagatcg ccaagagctt ttcgcgcggc gagccgcggg cgatgtccgg gcctgccgcc
    12721 tcgtgaaacg tccggaacgc gtcggcgatc agcgcgagct tgtccggcca atggcggtag
    12781 atcgtcgatt tgccgacgcc tgcccgggcg gcgaccgact cgatggcgaa gccgccatag
    12841 ccgatctcgc cgagttcctc gagcgcggcg ccgaggatca cttggcgcga gcgctcgatg
    12901 cggggatcga ccgccggctt gagaggcgac gctcgcgtca acttgctggc gccgctccac
    12961 ccgagcctgg cgcgcggtag tcggccgtat tcggctggaa cagctcgacg agattgcccg
    13021 acgggtcttc caggagaatc tgcttgccgc cgacgccctg cacgacctcg ttgcggaatc
    13081 ggcaacccgc cccgttgagc cgcgcgatca ccgcatcgag gtcgtcgacg acgagatgaa
    13141 cgcggttcca gccgccgggc gtctgctcct cgcccgaagg catcgccgcg ccaccgccca
    13201 tgcgcgatgg ctgagtcaga tagatgtgca tgtcgccgcg cgagatctcg gcgaagggcg
    13261 gcgccgggtg catctccagc ttgaagccca gccgcttcgt ataaaactcg atcgacgcat
    13321 cgacgtcgtc gacgatgtag cgaaagcaag ccggcatgag gcgtcctccc tattccggcc
    13381 actccacgct ccgcagcatc ggagaagccg tcatccatga gggtggcgtg tctagatatg
    13441 aaacgtatcc gtttcgtagc gatacgtcaa cgtttcgtat ctaatgcagc tttacctccg
    13501 gctccgcgcc gcgcgagagc tggcgcgcga gcgcgccgag gaaggtcctg ccgccgccct
    13561 gcagcgcgta ttcgtgcatc ttgtagagcg agcggtacat cagccgcgcg aaataacctt
    13621 cgatgaacag gttcttgccg atcaggaagc ccatcaggtt gccgacggtg ctgtagtcgc
    13681 cgagcgagac cagcgagccg aaatcgcgat agacgaaagg ctcgagcggc tgtcccgcga
    13741 gacggcggcg cagctgccgc agcagatgcg acgcctgttg atgcgccgcc tgggcgcgcg
    13801 gcggcaccgg cgcaggatag ccctcgcgcg ggcaggaggc acaatcgccc atcgcgaaaa
    13861 tgtcggggtc cttcgtcgtc tgcagcagcg gcgtcaccac gagctggttg aggcggttga
    13921 cctcgagccc gtcgaggtcg cgcagcacct cgggggcctt gacgccggcg caccacacga
    13981 cgagctcgga cgggatgaaa gcaccaccct cgagaacgac gccgtcggcg cgaacctcgc
    14041 tgacccgggc cccggccctg acctcgacgc cgagagtcct gagcagcgcg accgtcgcgt
    14101 cggcgacgcg ctccggcagc gccggcagga tgcgctccgc ggcctcgatc aggatgatgc
    14161 gcaaatcctt ttcggggtcg atgcggtcga ggccgaaggc gaccacctgg cgtgccgtgc
    14221 gatgaagttc ggccgccagc tccgtgccgg tcgcgccggc gccgatgacg gcgacatgga
    14281 gctgccccgg gcgcaccggc tctgcctgcg catcggcgcg gatacaggca ttgacgaggc
    14341 ggtgattgaa gcgcaccgcc tgctccggcg tgtcgagcgg caccgcgaag cgcgccgcgc
    14401 cggctgtccc gaaatcattg gtgacgctgc cgatcgcgat caccagcgtg ccatagccga
    14461 tggcgcgggg cggcgtgatc tgccgtcctt cctcgtcgaa ggtcggggcg agacggacct
    14521 ccttcttctt tcggtcgagg ccgatcatct cgccgaggcg atattggaaa tgatgccaat
    14581 gcgcctgcgc caagtagttg agctcatgct cggcgggatc catgctgccg gccgccaccg
    14641 catgcagcag tggcttccag agatgcgtgc gcgccttctc gacgagcacg atctgcgcct
    14701 gcccgctttt gccgagcgta ttgccggcgc gcgtcgcaag ctcgagcccg cccgcgccgc
    14761 cgccaacgac gacgatggga tgccgatcgt ccatgccgat gaccatagag gttcaggaaa
    14821 gaagaagcac ggccacccct ctcccgcgtt gcgggagagg gagggaccca ttgcgaagca
    14881 atgggagggt gaggggaccg cggc
//
LOCUS       DASYNS010000518         6157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5588543, whole genome shotgun sequence.
ACCESSION   DASYNS010000518 DASYNS010000000
VERSION     DASYNS010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5588543"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1776
                     /locus_tag="VGP50_16055"
     CDS             181..1776
                     /locus_tag="VGP50_16055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEV8028948.1"
                     /translation="MAWNLVSPPSRNRVPAKRRAGAAAALTAAAFAAGCAVGPDFARP
                     APPSGAGYAPQPLPEVTASAAIHGGEPQHFVAGQDLPFEWWELFQSAPLNSLVAKALR
                     ANPTVPAAQAALRQAEEIVDAQQGFFFPTIAAGYQFERQKLAGNLSGSSAPGVQGNGT
                     AILATQNPNQSPHNKPLFFNFHTAQVTVSYAPDVFGLNRREVESLDAQAALARFELEA
                     TYITLASNVVAAAIQEASIRAQIAATRDIIDFDEKALDILRKQLRLGFAMRIDVAAAE
                     AALAQAKQLLPPLENQFEQTRDLIRALVGNLPNEDVEETFELTSLQLPHDVPLSLPSK
                     IVDQRPDVRAAEEQLRSANAQVGVAIANRLPQFTLTGAAGGTATQFTQMFSPGGPFWS
                     IIGGVTQPLFDGNTLLHRERAADQALIEAAAQYRSTVLGAYQNVADTLHALKSDADAL
                     AAALEAERAARVTLDLTQRQMDVGYVNYLTLLNAEITYQQAALSLIQAQATRFGDTAA
                     LFQALGGGWWNRKLASAQADEHR"
     gene            1839..2507
                     /locus_tag="VGP50_16060"
     CDS             1839..2507
                     /locus_tag="VGP50_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV8028949.1"
                     /translation="MKLLLVEADPFVSAWLKKVLDIAGFDVDVAVGTTDGMASTVARA
                     RYPIVMIGPQTPASDRAKLVRLFYAQPDRAAPLLWVAPFNGDRDGAMTSSPGAYFRDA
                     LQVEALATRLRALLMLKRDDLGAVLRAGNVTLDTAARQVVVDKKVTFFPSAEVVVLAM
                     LMQHEGQVVPKKLIEIRLWGTTDKRAKNAVEVCIHRLRKRLATAAASVRVRTIHRVGY
                     FISE"
     gene            complement(2494..3003)
                     /locus_tag="VGP50_16065"
     CDS             complement(2494..3003)
                     /locus_tag="VGP50_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028950.1"
                     /translation="MRRRRRRPEKPFHLVIVDHDNDRYTLEGPMTDAEAWIREVVAAR
                     KAGRLITCRVLVMGAEEIEAWAAQREDGTRWPSGFIVAPPEVAKAAPAVETLGGPDGS
                     TPRLEGALEVTSVMVLEALRSLYRSNQLKDEAPGASEATVTTPAITSSERRAAAMVGG
                     DLSAAAIRR"
     gene            complement(3117..3464)
                     /locus_tag="VGP50_16070"
     CDS             complement(3117..3464)
                     /locus_tag="VGP50_16070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028951.1"
                     /translation="MFKLLTTIAVVGLATQIGAGPAAAQSASCNAAMARLMDQWHAVG
                     YATPEKPTQARVLGNDGHEATGQQVSYMHTQIELAAQECQQGDSAAALHRIASVSGML
                     DGEGVRTASRTAQ"
     gene            4081..4842
                     /locus_tag="VGP50_16075"
     CDS             4081..4842
                     /locus_tag="VGP50_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV8028952.1"
                     /translation="MTKHPATVLVVDDELPIRRLLKRKLASQNYQVIEAETGKAALDL
                     IKREDPDVVILDLGLPDMDGLDMIPLIREHSRVPIVVLSSRGDERVKVEALLRGADDY
                     VTKPFGMDELVARLYTALRHRFHEQGQEPVFRTGELMVDLVHRRITLAGNEVKLSPTE
                     FDILRLLVSHAGKVLTHPQILQEIRGHGADDVQYLRVYMRLLRQKLEPDPVQPRYIVT
                     EPGVGYRLQILDQPAEPVRGSAAGRGSASGPAAAK"
     gene            complement(4730..>6157)
                     /locus_tag="VGP50_16080"
     CDS             complement(4730..>6157)
                     /locus_tag="VGP50_16080"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028953.1"
                     /translation="FSALRYGLPCALLGAALGTLALDFFFIPPLYEFNIDDPRDTVAL
                     ACFAIVAVITSQLSGRLRAEMLTARHRARITAELYSFSRLLAGISAVDELLPAIATRI
                     GAMANARAALLIETKGHLALGASAPPGLGVDADAMARARQVLDEGEPVQGTEELLLPL
                     RTGHGAVGVLLLRRDAPALPLGAEERRLVDAVANQAAVAIERIRLAAEIEETRVLRET
                     ERLRSALLTSVSHDLRTPLSSVLAALANLRDDGVAADSAVRAELLDTAQEEGERLNRF
                     VGNLLDITRIEGGIIAPRREPADLADIVGSAVRHAAKLLTRHRLAIDLEPDLPMVRVD
                     FVLLEQVVFNLLDNVAKYAPPASTVSITGRASQGSIVLEIADEGAGIAESELERIFDK
                     FHRLEAADRQRAGTGLGLAICRGFVEAHGGSIVARNRLDRSGAVFTVTLPLPDPMPIR
                     DLPRSREPAPPADQESEGGSRRRAL"
BASE COUNT         1027 a         2082 c         2001 g         1047 t
ORIGIN      
        1 gagtcgcggc agcagcttct ttcgcatggc catgctccgt tcagaaaccg agcgaccatg
       61 cccgcatccg cggggtcgaa ctactgtgac aagtcgcagt gccgactgtg acttgtcaca
      121 gttccgcggc cggggaccgt agcgcagagt tcgcgcgccc atccttgcgc agcgaagccc
      181 ttggcctgga acctggtgtc gcctccttca cgcaatcgcg tcccggcgaa gcgccgcgcc
      241 ggcgccgcag cagcattgac ggcggccgct ttcgccgccg gctgcgcggt cggccccgac
      301 ttcgcccggc ccgcaccgcc ctccggcgcg ggctacgcgc cgcagccgct gccggaggtc
      361 accgcgtccg cggcgattca cggcggcgag ccgcagcatt tcgtggcggg gcaggatctg
      421 cccttcgaat ggtgggagct gtttcaatcg gcgccgctca attctctcgt cgccaaggcg
      481 ttgcgcgcca acccgacggt gccggccgcg caagcggcct tgcgccaggc ggaagagatc
      541 gtcgatgcgc agcagggctt cttctttccg acgatcgccg cgggctacca gttcgagcgc
      601 cagaagctgg ccggtaatct ttccggcagc agcgcgccgg gcgtgcaggg caacggcacg
      661 gcgatcctgg cgacgcagaa tccgaaccag tcaccgcaca acaagccgct gttcttcaat
      721 ttccacactg cgcaggtgac ggtcagctac gcgcccgacg tgttcggcct caaccgccgc
      781 gaggtcgagt ccctggacgc gcaggcggcg ctggcgcgat tcgagctcga ggcgacctac
      841 atcacgctgg cctccaacgt cgtcgccgcg gcaatccagg aggcttcgat acgcgcgcag
      901 atcgccgcga cgcgcgatat catcgatttc gacgaaaagg cgctcgacat cctgcgcaaa
      961 cagctgcggc tgggatttgc catgcgcatc gacgtggccg cggcggaagc ggcgctggcg
     1021 caagccaagc agctgctgcc gccgctcgaa aatcaattcg agcagacgcg ggacctcatt
     1081 cgagcgctgg tcgggaacct gcccaacgag gacgtcgagg agaccttcga gctcacgtcg
     1141 ctacagctgc cgcatgatgt gccgctcagc ctcccctcga agatcgtcga ccagcgcccc
     1201 gacgtccgcg ccgccgaaga gcagctgcgc tcggccaatg cgcaagtggg cgtggccatc
     1261 gccaatcggc tgccgcaatt cacgctcacc ggcgccgccg gcggaacggc gacgcagttc
     1321 acacagatgt tcagtcccgg cgggcccttc tggagcatca tcggcggcgt cacccagccg
     1381 ctcttcgacg gcaacacgct gctgcatcgc gagcgcgccg ccgatcaggc gttgatcgag
     1441 gcggcggcgc agtatcgcag caccgtgctg ggcgcctacc agaacgtcgc cgatacgctc
     1501 cacgccctca aatccgatgc cgatgcgctg gcggcagcgc tcgaggccga gcgcgccgcg
     1561 cgggtcaccc tcgatctcac gcagcggcag atggatgtcg gctacgtcaa ctacctgacg
     1621 ctgctcaacg ccgagatcac ctatcagcag gcggcgctca gcctgataca ggcgcaggcg
     1681 actcggttcg gcgacacggc tgctctgttc caggcgctcg gcgggggctg gtggaaccgc
     1741 aagctggcct cggcacaggc cgacgagcac cgttgaccga gagacggggt gcctatcgac
     1801 cgcgcggcgg agcgcgcggc gttgccgaga gcagagcaat gaagttgttg cttgtcgagg
     1861 cagatccatt cgtgtcggcc tggctcaaga aggtcctcga tatcgccggc ttcgatgtcg
     1921 acgtcgccgt cggcaccacc gacggcatgg cgagcacggt cgcgcgggcg cgatacccca
     1981 tcgtcatgat cgggccgcaa acgccggcca gcgatcgggc gaagctcgtg cgcctgttct
     2041 acgcgcaacc tgatcgagcg gcgccgctcc tctgggtcgc gccgttcaat ggcgatcggg
     2101 acggcgccat gacgtcgtcg ccgggcgcgt atttccgcga cgccctccag gtcgaggcgc
     2161 tggcgactcg ccttcgcgcc ctgctgatgc tgaagcgcga cgatctggga gcggtgcttc
     2221 gcgccggcaa tgtcacgctc gacacggccg ctcggcaagt cgttgtcgac aaaaaagtga
     2281 ccttctttcc gtcggccgag gtggtcgtcc tcgcgatgct gatgcagcac gagggccagg
     2341 tcgtgccgaa gaagctcatc gagatccgat tgtgggggac gaccgacaaa cgcgccaaga
     2401 acgccgtcga ggtctgcatc catcgtcttc gcaagcgctt ggcaaccgcg gctgccagcg
     2461 tgcgggtgcg cacgattcac cgagtgggct atttcatctc cgaatagccg cggcgctcaa
     2521 atccccgcca accatggccg cagcacgacg ctcgctggac gtgatcgcag gcgtcgtcac
     2581 ggtcgcctcg ctcgcgcccg gcgcttcgtc cttgagctgg ttcgagcggt agagactccg
     2641 cagggcctcg agcaccatga ccgacgtcac ttcgagcgcg ccctcgaggc gcggcgtcga
     2701 tccatccggt ccgccgagcg tctccacagc cggcgccgct ttcgccacct ccggcggcgc
     2761 gacgatgaag ccggacggcc accgcgtgcc gtcttccctt tgcgcggccc aggcctcgat
     2821 ctcttccgcg cccatgacga ggacgcgaca cgtgatcagg cggccggctt tccgagcggc
     2881 gacgacttcg cggatccaag cctccgcgtc ggtcatcggt ccttcgaggg tgtagcgatc
     2941 gttgtcgtgg tcgacgatca ccagatggaa tggtttctcc ggccgccgcc ggcggcggcg
     3001 cacgaccggg agagacggcg tcgagaactc tcttccccgt cgctctcccg cttcactctg
     3061 cggcaatgtc ggcgcaacgt cgaacgctgc gctcgtccgg cttgcagccg taagcgtcat
     3121 tgcgccgtgc ggctggcggt ccggacgccc tcgccatcca gcatgccgct gacgctggca
     3181 atgcggtgca gcgccgccgc gctgtcgccc tgctggcact cttgcgcggc gagctcgatc
     3241 tgcgtgtgca tatagctcac ttgctgaccg gtggcctcat gcccgtcatt gccgaggact
     3301 cgcgcctgag tgggcttctc cggcgtggcg tagccgacgg catgccactg atccatcaac
     3361 cgggccatcg cggcgttgca tgacgcgctc tgagccgccg ccggcccggc gccgatttga
     3421 gtcgcgagac cgaccacggc gattgtcgtg agaagcttga acatgacatt gactccttgg
     3481 ctgagacgcc ggcgcgaagg cgcggctgcc ttccagcact acaaagtctg ggtgttttga
     3541 agatagtgat ggtacccatc aaaaatcgag attggctgcg tcgcgaaatt ataaaaacgc
     3601 cagtaaaaat ccccatcttc tcattatcat cgctgccgat ttactgtgct catgaattaa
     3661 tatgtattct ttgcttgccg agcatgaggc attctcgcgg ggcggccggg aagtcggggg
     3721 acatgcgggc acttgaatgg gttgtccggt cgcccggaat ctctgggcag tggtattctt
     3781 agcgattctc gtccgctcga cggtcggaat atttctggat tttatatggt cagaggccaa
     3841 cgcccatctc gactttttat atgtatccat gctctctgaa cgctgcgata ctgcgcagcg
     3901 ctcggtgatc ggggtcacct cgccgtcatg tcgcgccagc tgcttcggcg gacgagcgtc
     3961 cgctcgccgg ggccgtcggc cttgtcatct ggccttcgag gaccgcatgt caccgtatag
     4021 ccccgactcc gagcgaccat tatcgcttgc gccttcccga ccgttcggcg ggagcccgtc
     4081 atgaccaagc atcccgccac cgttctcgtc gtcgacgatg aattgccgat ccgccgactc
     4141 ctgaaacgca agctcgccag ccagaactac caagtcatcg aggcggagac cggcaaggcg
     4201 gcgctcgacc tgatcaagcg cgaagatccg gacgtggtga tcctcgatct gggccttccg
     4261 gacatggacg ggttggacat gatcccgctc attcgcgagc attcacgcgt gcccatcgtc
     4321 gtcttgtcga gccgtggcga cgaacgggtg aaggtcgagg ccttgctgcg gggagcggac
     4381 gactacgtca ccaagccctt cggcatggat gagctggttg cccggctcta caccgccttg
     4441 cgccaccgct tccacgagca ggggcaggag ccggtgtttc gcacgggcga gctcatggtc
     4501 gacctcgtcc atcggcgcat cacgctggcc ggcaacgagg tcaagctgtc gccgaccgaa
     4561 ttcgacatcc tgcgcctcct ggtgtcgcat gccggcaagg tcctgacgca tccccagatc
     4621 ctgcaagaga tccgcggtca cggcgccgat gacgtgcagt atctgcgcgt ctacatgcga
     4681 ttgctgcggc agaagctcga gccggatccg gtgcagccgc gctatatcgt cacagagccc
     4741 ggcgtcggct accgccttca gattcttgat cagccggcgg agccggttcg cggctccgcg
     4801 gcaggtcgcg gatcggcatc gggtccggca gcggcaaagt aacagtgaag acggcgccgc
     4861 tgcggtcgag gcgattgcgc gctacgatcg atccgccatg ggcctcgaca aaaccgcggc
     4921 aaatggcgag gccaagtccc gtcccggcgc gttgacggtc cgccgcctcg agacggtgaa
     4981 acttgtcgaa gatccgctcg agctcgctct cggcgatgcc ggcgccttcg tcggcaattt
     5041 ccagaacgat cgaaccttgg gatgcgcggc cggtaatgct cacggtcgac gcagggggcg
     5101 cgtatttcgc gacattgtcg aggagattga agacgacctg ctcgagcagc acgaaatcga
     5161 cgcggaccat cggcaagtcc ggctcgagat cgatcgcgag gcgatgtctg gtcagcagct
     5221 tcgccgcgtg ccgcaccgcg ctgccgacga tgtctgcaag atcggccggc tcgcggcgcg
     5281 gcgcgatgat gccgccttcg atacgggtga tgtcgagcag atttccgacg aagcggttca
     5341 gccgctcgcc ctcctcttgc gccgtatcga gcagctccgc gcgcacggcg ctgtcggcgg
     5401 cgacgccgtc gtctcgcagg ttggccaggg cggcgagcac cgacgacagc ggggtcctga
     5461 ggtcgtgcga caccgaggtc aacaaggccg agcgaagccg ctcggtctcg cgcaagaccc
     5521 gggtttcctc gatttccgcc gcgagccgga tgcgctcgat ggcgaccgcc gcctgattcg
     5581 ccaccgcgtc gacgagccgc cgctcctcgg cgccgagggg cagggcaggc gcatcgcgcc
     5641 gcagcaagag cacgcccacc gccccgtgcc ccgtccgcag cggcagcagg agctcttcgg
     5701 tcccttgcac cggttcccct tcgtccagca cctggcgcgc tcgggccatc gcatccgcgt
     5761 ccacgccaag cccgggcggc gcgctggcgc cgagggcgag atgacccttc gtctcgatga
     5821 gaagcgccgc gcgcgcgttt gccatcgcgc caattcgcgt cgcgatcgcc ggcagcagct
     5881 cgtcgacggc ggagatgccg gcgagcagcc ggctgaatga gtagagctcg gcggtgatcc
     5941 tggcgcggtg gcgcgccgtg agcatctcgg cgcgcaagcg tccgctgagc tggctcgtga
     6001 tgacggcgac aatggcaaag caagcgagcg caaccgtgtc ccgagggtca tcgatgttga
     6061 actcgtagag cggcgggatg aaaaagaaat cgagcgcgag cgtgccgaga gcggcgccga
     6121 gcaacgcgca gggcaacccg tagcgcagcg ccgagaa
//
LOCUS       DASYNS010000519         1923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2470599, whole genome shotgun sequence.
ACCESSION   DASYNS010000519 DASYNS010000000
VERSION     DASYNS010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2470599"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VGP50_16085"
     CDS             <1..327
                     /locus_tag="VGP50_16085"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV8028954.1"
                     /translation="SQFKDATLPPVLATPVMIMMMENAALNAIKPYLDAGESAVGTHV
                     DVRHLAPTPVGRQVTAAARVTRAAGRHVEFDVWAMDGNEEIGKGSHARAVIEIAPFLK
                     RLAAKR"
     gene            complement(465..>1923)
                     /locus_tag="VGP50_16090"
     CDS             complement(465..>1923)
                     /locus_tag="VGP50_16090"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV8028955.1"
                     /translation="RAGVCLSTLGPGALNLTTGAAYALLGAMPMVMITGQKGILSRKQ
                     ARFQVVDIVATMTPLTKMARQIVSPATIPSILREAFRVAQQERPGPVHLELPEDIAGE
                     TAPAGLAPVPPHPIELPVAQGAALDRAAELILKAERPLIMLGAAASRPRLAEALSAFV
                     KRLQIPFFNTQMGKGAVTGGSGLYMGTAALSERDYVHRAIDRADLIIAIGHDTVEKPP
                     FFMGPGGPTVLHIGYLPATVEEVFFPHAEVVGDVGPGLMLLADRLEGRLPRAGALLAL
                     RAEILARVADRAEESRFPLTPQRIVHDVRQVMPEDGIACLDNGMYKIWFARNYRTRVA
                     NTLLLDNALATMGAGLPSAMMAAMLHPKRRVLAVCGDGGFMMNSQELETAVRLGLNLV
                     VLVLQDNAYGMIRWKQATDGYPDFGMTFGNPDFVAYAKSYGVRGTRVETADGLAPALA
                     AAFAGGGVHLVTVPVDYSENIRVLVEELRANTAGN"
BASE COUNT          334 a          651 c          643 g          295 t
ORIGIN      
        1 agccaattca aggatgcgac cctgccgccg gtgctggcga cgccggtcat gatcatgatg
       61 atggagaacg cggcgctcaa cgcgatcaag ccctatctcg atgccggcga aagcgctgtg
      121 ggcacgcatg tcgatgtgcg ccacctcgcg ccgacgccgg tcggccgcca ggtcacggcc
      181 gcggcacggg tgacgcgcgc ggccggccgc catgtcgagt tcgatgtctg ggcgatggat
      241 ggcaacgagg agatcggcaa aggctcgcat gcgcgcgccg tgatcgagat cgctccgttc
      301 ctgaagcgcc tcgccgccaa gcgctgaggc aagctctcag ctatcagctg tcagctcacc
      361 gaatgagcgg caacgccggt tcagaatcgc cgaccagctg catcgctgtc accatcgctc
      421 acaagagctg acggctgaca gctgatagct ccgacgccga cgcgtcaatt cccggccgtg
      481 ttcgcgcgca actcctcgac caggacccgg atgttctccg aatagtccac gggcaccgtg
      541 acgagatgga cgccgccgcc ggcgaaggcc gccgcgagcg ccggcgccag cccatcggcg
      601 gtctcgacgc gcgtgcccct gacgccatag gacttggcat aggcgacgaa atcgggattg
      661 ccgaaggtca tgccgaaatc gggatagccg tccgttgcct gcttccagcg gatcatccca
      721 taggcattgt cctggagcac cagcacgacg aggttgagcc cgagacgcac ggccgtctcc
      781 agctcctgcg aattcatcat gaagccgcca tcgccgcaca ccgcgagaac gcgtctcttc
      841 ggatggagca tggccgccat catggccgaa ggcagcccgg cgcccattgt cgcgagcgcg
      901 ttgtcgagga gcagcgtgtt cgcgacccgc gtgcgatagt tgcgggcgaa ccagatcttg
      961 tacatgccat tgtccaggca ggcgatcccg tcttccggca tgacctgccg cacgtcatgg
     1021 acgatgcgtt gcggggtcag gggaaagcgg ctttcctcgg cccggtcggc gacccgcgcg
     1081 aggatctcgg cgcgcagcgc gaggagcgcc ccggctctcg gcagcctgcc ttcgaggcgg
     1141 tcggcgagaa gcatcagacc ggggccgaca tcgcccacca cttcggcatg cgggaagaac
     1201 acctcctcga ccgtcgcggg gagatagccg atatgcagga ccgtcggtcc gccgggcccc
     1261 atgaagaaag gcggcttctc caccgtgtcg tggccgatgg cgatgatcag atcggcacgg
     1321 tcgatcgcgc gatgaacata gtcgcgctcc gaaagcgcag cggtgcccat gtaaaggccg
     1381 gatccgccgg tcaccgcgcc cttgcccatc tgcgtgttga agaacgggat ctgcaggcgc
     1441 ttgacgaagg ccgagagcgc ctcggcaaga cgcgggcggc tcgccgccgc gcccagcatg
     1501 atgagcggcc gctccgcctt gaggatcagc tcggccgcac gatcgagcgc cgctccctgc
     1561 gcgaccggga gctcgatcgg atgcggcggg accggagcga gtccggccgg cgcggtctcc
     1621 cccgcaatgt cctcgggcag ctcgagatgg accgggcccg gcctctcctg ctgcgccacg
     1681 cggaacgcct cgcgcaggat cgacgggatc gttgccgggc tgacgatctg ccgggccatc
     1741 ttggtgagcg gcgtcatcgt cgcgacaatg tccacgacct gaaagcgcgc ctgcttgcgg
     1801 ctcaggattc ctttctgtcc ggtgatcatc accatgggca tcgcgccgag cagcgcataa
     1861 gcggcgccgg tggtcaggtt gagcgcgccc gggccgagcg tcgagagaca gaccccggcc
     1921 ctg
//
LOCUS       DASYNS010000520         3540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2158030, whole genome shotgun sequence.
ACCESSION   DASYNS010000520 DASYNS010000000
VERSION     DASYNS010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2158030"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VGP50_16095"
     CDS             complement(<1..312)
                     /locus_tag="VGP50_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028956.1"
                     /translation="MLFLWESFGPHHVDRCEACAAYFSDRYDVFGVEIATFDATYEWR
                     GSIGSRHFSKVTLFPGVVSQKIGGLRCFYRIMTTCLRLRAKYLFFCNYNIPAVFFSAV
                     VL"
     gene            complement(569..1396)
                     /locus_tag="VGP50_16100"
     CDS             complement(569..1396)
                     /locus_tag="VGP50_16100"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV8028957.1"
                     /translation="MAATEGRRLAGRVAIVTGAGSRAEGIGNGRAAAILLARHGARVA
                     LLDTMREWAEATARMIEAERGDCVVLAADVTDPAACAGAVEQTVARWGGLDILVNNVG
                     IAGPPGTAVEVDPAAWDQAMRVNVTSMMLMAKYAIPEMRKRGSGAIVNIASVAGLTGG
                     HPNLLYPTSKGAVVNLTRAMAAHHGAEGIRVNCVAPGMVYTPMVSSRGMTPEMREARR
                     NRSLLKTEGTGWDVGNAVVYLASDEARWVTGMVLPVDAGATAGASRVAVPPSGSHRA"
     gene            1606..2469
                     /locus_tag="VGP50_16105"
     CDS             1606..2469
                     /locus_tag="VGP50_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEV8028958.1"
                     /translation="MRVVLFCGGLGMRLRDYSDAIPKPMVPIGYRPLIWWVMKYYAHF
                     GHRDFILCLGYKGDVIKEYFLNYNECRSNDFVLSQGGQRVDLLQKDIDDWRITFVDTG
                     MSANIGERLMAVKPFLRDEDVFLANYSDGVSDFPLPVLTDEFLRSDKVATFLSVRPNQ
                     SFHFVNRRDDGLVTSIDDGANTWINGGCFVLRREIFDYMQPGDELVVEPFQRLIAEQK
                     LATHAYDGFWRCVDTFKDLQALESLYAKGHPPWELWRREPGRAAPAPAVALRPDEREP
                     AEAWQVANGAR"
     gene            2480..3124
                     /locus_tag="VGP50_16110"
     CDS             2480..3124
                     /locus_tag="VGP50_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEV8028959.1"
                     /translation="MLGLTFADRRQPLRLLCLGAHADDIEIGCGGTILRLAAEHPQMT
                     VRWIVFSGHGARRAEAEAAARSFLAGLADTAVTVHGFRDGFFPSQHAEIKEVFERLKR
                     EYSPSLVLTHYRADLHQDHRLIAELTYNTFRDHLVWEYETPKYDGDLGNPNLFSPLSE
                     DLARRKVSALLEHFPSQRDHSWFTAETFFAMLRLRGIGCNAPLAEAFYCRKMVV"
     gene            3340..>3540
                     /locus_tag="VGP50_16115"
     CDS             3340..>3540
                     /locus_tag="VGP50_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028960.1"
                     /translation="MSGEATRDGRNEDTVAGLLLRRLQAAGIDYFFANAGTDFPPIIE
                     GFARAAETGTALPQPLLVPHENA"
BASE COUNT          575 a         1207 c         1153 g          605 t
ORIGIN      
        1 aagcaccacg gctgagaaga agaccgccgg gatgttatag ttgcagaaaa atagatactt
       61 ggctcggagc cgcaggcacg tcgtcatgat ccggtagaag cagcgcagcc cgccgatctt
      121 ctggctgacg acgccgggga agagcgtgac cttgctgaag tggcggctgc cgatgctgcc
      181 gcgccattcg taggtggcgt cgaaggtggc gatctccacg ccgaagacgt cgtagcggtc
      241 gctgaaatag gcggcgcagg cttcgcaccg gtcgacatga tgggggccga agctttccca
      301 caagaagagc atccgcggca gctcaggcga agcttcccgt gccggcgccc cggcacccgc
      361 cacagccctt gccgcagacg ccgaaccggt gcccccccac cgcggcgccg tatattggta
      421 gcgcagggac ggggcttgtt cgcggggctt tactttctcg ttcaaggatt tgcgcgcgct
      481 gctgctcgaa tttgcctgtt aacagccttg ctgcatcgag gttccggcag ttctgcgccc
      541 gccgtgcttt tcgccttacg gttgagagct acgcgcgatg gctgccgctc ggtggtacgg
      601 ccacgcgcga ggcgccggcg gtggcgccgg catcgactgg gaggaccatt ccggtgaccc
      661 agcgcgcctc gtcactggcc aaatagacga cggcgttgcc gacgtcccag ccggtcccct
      721 cggtcttgag caacgagcgg ttgcgccgcg cctcgcgcat ctccggcgtc atgccgcggc
      781 tcgagaccat gggcgtgtag accatgccgg gcgcgacgca gttgacgcgg atgccctccg
      841 cgccgtgatg cgcggccatg gcgcgcgtca ggttgacgac cgcccccttg gaggtaggat
      901 acagcaggtt cggatggccg ccggtcagcc ccgcgaccga agcgatgttg acgatggcgc
      961 ccgagccgcg tttgcgcatc tcgggaatgg cgtatttcgc catcagcatc atcgaggtca
     1021 cgttgacccg catcgcctgg tcccaggcgg cgggatcgac ctcgaccgcc gttccgggcg
     1081 ggccggcgat gccgacattg ttgaccagga tgtcaagccc gccccagcgg gccacggtct
     1141 gttcgacggc gccggcacag gcggcgggat cggtgacatc ggccgcgagc acgacacagt
     1201 cgccgcgctc ggcctcgatc atccgcgccg tcgcctcggc ccattcccgc atcgtgtcga
     1261 ggagcgcgac ccgcgcgccg tggcgcgcca acaggatggc cgcggcccgc ccattgccga
     1321 tcccctcggc gcgcgagccc gcgccggtga cgatcgcaac cctgccggcg aggcgccggc
     1381 cctcggtagc cgccatgagg ccatattgaa gcgtgtcacc cggcggggcg caaccggcaa
     1441 aggcctgact cccccggggc cgattggagg gaacaatctg cgcacggaag tgttctctgg
     1501 gccggtgtcc tggcgggagc catcagcggg aacgcgagcg cgtttgacag ccgcagccgc
     1561 gtcatcccga gagcccgcca ccgatacggg caagcgaggt aggcgatgcg cgtggtgctg
     1621 ttctgcggcg gtctcggaat gcggctgcgc gattattccg acgccattcc aaagccgatg
     1681 gtgccgatcg gctatcgccc cttgatctgg tgggtgatga agtactacgc gcatttcggt
     1741 caccgcgact tcattctctg cctcggctac aagggcgacg tgatcaagga gtatttcctc
     1801 aactataacg aatgccgctc caacgacttc gtgctgtcgc agggcggcca gcgcgtcgac
     1861 ctgctgcaga aggacatcga cgattggcgc atcaccttcg tcgacaccgg gatgagcgcc
     1921 aatatcggcg agcggctgat ggcggtgaaa cccttcctgc gcgacgagga cgtgttcctg
     1981 gccaattaca gcgacggcgt cagcgatttt ccgctgccgg tgctgaccga tgagttcctg
     2041 cgcagcgaca aggtcgccac cttcctgagc gtgcgtccga accagagctt ccacttcgtc
     2101 aaccggcgcg atgacggcct cgtcacgtcg atcgacgatg gcgccaatac gtggatcaat
     2161 ggcgggtgct tcgtgctgcg ccgcgagatc ttcgactata tgcagccggg cgacgagctg
     2221 gtggtcgagc ccttccagcg cctcatcgcc gagcagaagc tcgcgaccca cgcctatgac
     2281 ggcttctggc gctgcgtcga tacctttaag gacctgcagg cgctcgaaag cctttacgcc
     2341 aagggacatc cgccttggga gctttggcgg cgagagcccg ggcgcgccgc gccggcgccc
     2401 gccgtcgcgc tccgaccgga cgagcgcgag cccgccgagg cttggcaggt cgccaacggc
     2461 gcgcgctgag cgttcgccca tgctcggcct cacctttgcc gatcgccgcc agccgctgcg
     2521 tctgctctgc ctcggcgcgc atgcggacga catcgagatc ggctgcggcg gcacgatctt
     2581 gcgtctcgcc gccgagcatc cgcaaatgac cgtgcgctgg atcgtgttca gcggccacgg
     2641 cgcgcgccgc gccgaagccg aggcggcggc gcgctctttt ctggcggggc tcgccgacac
     2701 cgcggtgacg gtgcatggct ttcgcgacgg cttctttccg tcgcagcacg cggaaatcaa
     2761 agaggtcttc gagcggttga agcgcgagta cagcccgtcg ctcgtcctca cccattatcg
     2821 cgccgacctg caccaggacc atcgcctgat cgccgaactc acctacaaca ccttccgcga
     2881 ccatctggtc tgggaatacg agacccccaa atacgatggc gacctcggca atcccaatct
     2941 cttctcgcct ctgagcgagg atctcgcgcg gcgcaaggtg tccgcactgc tcgagcattt
     3001 tcccagccag cgcgatcatt cgtggttcac cgccgagacc ttcttcgcca tgctgcggct
     3061 gcgcggcatc ggctgcaacg cgcccctcgc tgaagccttc tattgccgta agatggtggt
     3121 ctgagccggt ccgctgcgcc ctcccggcac agccatttca tacccggcgt ggcagagact
     3181 gtcgacctct accggggccg ccttcgactg ttccgctttc atcccagagc gagcttggac
     3241 cgccggccct cgcgcatgag gcgcatcgcc aacgctcgcg cgccgcttga cgggtaaccg
     3301 gagccggcgg acagtcgggg aggccgacag gagcggatga tgagcggcga agcgacgcgc
     3361 gacggccgga acgaggacac ggtggccggc ctgttgctgc gccgtctcca ggcggcgggc
     3421 atagattatt tcttcgccaa tgccggcacc gatttcccgc cgatcatcga aggcttcgcg
     3481 cgcgctgccg agaccggcac agcgctgccg caaccgctgc tggtccccca tgagaacgcc
//
LOCUS       DASYNS010000521         4117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2158388, whole genome shotgun sequence.
ACCESSION   DASYNS010000521 DASYNS010000000
VERSION     DASYNS010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2158388"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VGP50_16120"
     CDS             complement(<1..625)
                     /locus_tag="VGP50_16120"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV8028961.1"
                     /translation="MDYHTCPNLAAMFFDWAAERGERPFLWAKHDGAYHAITWTEATR
                     RVSMLARGLRALGVKAGDRVALIAENRPEWTIADLAIMTAGAITVPAYTTHTAEDFRH
                     VLANSGARAVIVSTASLANRVMPAADQVSTIASIITIEPLTRGQLSHAEIYLWDDVMA
                     RGAALPDEVASWVKAIGRDDIACLIYTSGTGGVPKGVMTTHANILANC"
     gene            complement(744..2123)
                     /locus_tag="VGP50_16125"
     CDS             complement(744..2123)
                     /locus_tag="VGP50_16125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEV8028962.1"
                     /translation="MSRSIAPEGAAATARPGELLGHPKGLTFLFATEMWERFSYYGMR
                     ALLVLYMVKYVLHPGHFESVVGFAALKSALETLSGPLGAQPLASEIYGLYTGLVYLTP
                     IFGGLLADRVLGRRRTVVLGAVLMVFGHFMMAFEPLFLAALLVLILGNGAFKPNISTQ
                     VGRLYAPGDPRRDRAYSIFYVGINLGAFLAPLVCGTLGEDVGWSYGFGAAGIGMSIGL
                     AIYLWGLRALPSDEPHWPHQEKASLGREEWRAVAALLVLFLPTALFWAAYEQQGNTIV
                     LWADDFTDRSVNLFFWQGEIPTTWFQAFNPFMIFAFTPLVVAFWSAQARRGAEPSTVA
                     KMAMGCLGCALAYAILALAAREAGDGKASWLWLLGYFVVITVAELYLSPIGLSLVTKV
                     APASILSLMMGLWLATSFVGNFMAGWLGGFWSGMHRPGFFLMIAGVSALGAAAIWSVR
                     STLRSALER"
     gene            2256..4055
                     /locus_tag="VGP50_16130"
     CDS             2256..4055
                     /locus_tag="VGP50_16130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEV8028963.1"
                     /translation="MTAKPAASARADTLGPLPEWDLADLYPGRDSPELKADLARSQGA
                     ASAFRQRYAGKLAGLSGPALAGAIAEFEQVQDLLGRIMSYASLVHAGDMSHPEIGRFF
                     QNIQERVNAISTELLFFTLEINRIDEAALGQKLESRELAHYAPWLRDTRAFRPYQLSD
                     EIEKLLHEKYVAGRAAWTRLFDETGAGLRFAIDGEELTSAEALHLLSDPDGEKRKAAA
                     KAIGAVYGKNIRLFALITNTLAKDKEIEDRWRGFKRPISSRNLANVVADEVVDALIAA
                     VRQSYPSLSHRYYRLKARWFGRDQLPYWDRNAPLPEQEDRIIPWSEAERIVLGAYGAF
                     SPELAAIGRRFFEKRWIDAPVRPGKAPGAFAHPTVPSAHPYLLLNYQGKTRDVMTLAH
                     ELGHGVHQVLAAPQGALMADTPLTLAETASVFGEMLTFRALLAAERDPVRRKTLLASK
                     VEDMLNTVVRQIAFVEFERRIHDERRESELTPERIGEIWLDVQRESLGPAIAFDDDYR
                     AYWSYIPHFIHTPFYVYAYAFGDCLVNSLYAVYQNAAPGFAEKYLDMLSAGGTRRHRE
                     LLAPFGLDASDPAFWKKGLSVISAFIDELEKMG"
BASE COUNT          754 a         1383 c         1361 g          619 t
ORIGIN      
        1 agcaattcgc gagaatgttg gcgtgcgtcg tcatcactcc cttgggcacg ccgccggtgc
       61 ccgaggtgta gatgaggcag gcgatatcgt cgcggcctat cgccttgacc caggacgcga
      121 cctcgtcggg aagcgccgcg ccgcgcgcca tcacatcgtc ccagagatag atctcggcgt
      181 gggagagctg ccctcgggtc aacggttcga tggtgatgat gctcgcgatg gtcgagacct
      241 ggtcggcagc gggcatcacg cgattcgcca ggctcgcggt cgagacgatc acggcgcgcg
      301 cgccgctgtt ggcgaggacg tgacggaaat cctcggccgt gtgggtcgtg taggccggga
      361 cggtgatcgc gcccgccgtc atgatggcga gatcggcgat cgtccattcg ggccggttct
      421 cggcaatgag ggcgacacgg tcgccggctt tgacgccgag cgcgcgcaag ccccgcgcca
      481 gcatgctcac gcgtcgcgtc gcctcggtcc aggtgatcgc gtgataggcg ccgtcgtgct
      541 tcgcccagag gaacggccgc tcgccgcgct cggccgccca gtcaaaaaac atggcggcga
      601 ggttggggca ggtgtggtag tccatgcgcc tcccttgtcg acaccgcctg gcccgcccgg
      661 tcggccgtgt ctttggcggc gttgtagggc gtttgacggc gcggcgaaat agcccgacgc
      721 acgacttctt gcgtagcgcc gctctatcgc tcgagggccg atcggagggt cgagcggacg
      781 gaccagatcg cggcggcgcc cagcgcggag accccggcga tcatcaggaa gaaacccggt
      841 cggtgcatgc cgctccagaa accgccgagc catcccgcca tgaagttgcc gacgaaactc
      901 gtcgccagcc acaggcccat catcagcgag agaatggagg ccggcgcgac cttggtgacg
      961 agcgacaggc cgatcggcga gaggtagagc tcggccacgg tgatgaccac gaaatagccg
     1021 aggagccaca accagctcgc tttgccgtct cccgcctcgc gggcggcgag cgccaggatc
     1081 gcatacgcaa gcgcgcagcc gagacaaccc atcgccatct tggcgacggt cgacggctcg
     1141 gcgccccgcc gcgcctgggc cgaccagaaa gcgacgacga ggggcgtgaa ggcgaagatc
     1201 atgaagggat tgaaagcctg gaaccaggtg gtggggatct cgccctgcca gaagaaaagg
     1261 ttgaccgagc gatccgtgaa atcgtcggcc cagagcacga tcgtgttgcc ttgctgctcg
     1321 tatgccgccc agaacagcgc cgtcggcagg aagaggacga ggagcgccgc gaccgctcgc
     1381 cactcctccc gccccagcga tgctttctcc tgatgcggcc agtggggttc gtccgacggt
     1441 agagcgcgca atccccagag atagatggcg agaccgatgg acatgccgat gccggccgcg
     1501 ccgaagccat agctccagcc gacatcctcg ccgagcgtgc cgcagacgag cggcgccagg
     1561 aatgcgccaa gattgatgcc gacatagaag atcgaatagg cgcggtcgcg tcgcggatcg
     1621 cccggcgcat agagcctgcc gacctgcgtc gaaatgttgg gcttgaaggc gccgttcccc
     1681 aggatcagca cgagaagcgc cgccaggaac agcggctcga aggccatcat gaaatgcccg
     1741 aacaccatga gaacggcgcc gaggacgacc gtgcggcgcc ggcccagcac gcggtcggcc
     1801 aggagcccgc cgaagatcgg ggtcaggtag acgagcccgg tgtagagacc gtagatctcc
     1861 gaggccaagg gctgggcgcc gagcggcccg gacaacgtct cgagcgccga tttgagcgcg
     1921 gcgaagccga ccacgctttc gaagtgcccg ggatggagaa catatttcac catgtagagc
     1981 acgaggagcg cccgcatccc gtagtaggaa aagcgctccc acatctcggt cgcgaagagg
     2041 aaggtcaggc ctttgggatg gccgagaagc tcgcccggcc gggcagtggc ggcggcacct
     2101 tcaggagcaa tcgaacgcga cattctagac tcgtcccaaa agggcgcaga accggttgcg
     2161 gctccaagaa cggccgcgct tggcggcgct ccaggccggc cctatatgca ggggcgaaac
     2221 gggaggaagc aaccatggcg acgacgcacc aggagatgac ggcgaaaccc gcggccagcg
     2281 cccgggcgga cacgcttggc ccgcttcccg aatgggatct ggccgacctc tatcccggcc
     2341 gcgacagccc cgagctcaag gccgatctgg cgcgcagcca gggcgccgcg agcgcctttc
     2401 gccagcgcta tgccggcaag ctcgctggcc tgagcggccc ggcgctggcc ggcgccatcg
     2461 ccgaattcga gcaggtccag gatctgctgg gccgcatcat gagctacgcc tcgctcgtcc
     2521 acgccggcga catgagccac cccgagatcg gccggttctt ccagaacatc caggaacgcg
     2581 tcaacgccat ctcgaccgaa ctcctgttct tcacgctcga gatcaaccgc atcgacgagg
     2641 ccgcgctggg gcagaagctc gaaagccgtg agctcgcgca ctatgcgccc tggctgcgcg
     2701 acacccgcgc cttccggccc tatcagctca gcgacgagat cgagaagctg ctgcacgaga
     2761 agtatgtcgc gggccgcgcc gcctggacgc ggctcttcga cgagaccgga gcgggcttgc
     2821 gtttcgccat cgatggcgag gagctgacca gcgccgaggc gctgcatctc ctctccgatc
     2881 ccgacggcga aaagcgcaag gccgcggcca aggcgatcgg cgccgtctat ggcaagaaca
     2941 tccgcctctt cgctctcatc accaacacgc tcgccaagga caaggagatc gaggaccgct
     3001 ggcgcggctt caagcggccg atctcgtcgc gcaacctggc gaatgtcgtc gcggacgagg
     3061 tggtggacgc gctcatcgcc gccgtgcgcc aatcctaccc gtcgctgtcc catcgctact
     3121 accggctcaa ggccagatgg ttcggacggg accagctgcc ttattgggac cgcaacgcgc
     3181 ccttgccgga gcaggaggac cggatcatcc cctggtccga ggccgagcgc atcgtgctcg
     3241 gcgcctatgg cgccttctca ccagagctcg ccgccatcgg gcggcgcttt ttcgagaagc
     3301 gctggatcga cgcccccgtg cggccgggga aggcacccgg cgccttcgct catcccaccg
     3361 tcccgagcgc gcatccctat ctgctcctga actaccaggg caagacgcgc gacgtcatga
     3421 cgctcgccca tgagctcggc catggcgtgc accaggtgct ggcggcaccc cagggcgcgc
     3481 tcatggcgga cacgccgctg acgcttgccg agaccgcctc ggtgttcggc gagatgctga
     3541 ccttccgcgc gctcctggcg gcagagcgtg atcccgtccg gcgcaagacc ttgcttgcgt
     3601 ccaaggtcga ggacatgctc aataccgtcg tgcgccagat cgccttcgtc gagttcgagc
     3661 gccgcatcca cgacgaacgg cgcgagagcg agctgacgcc cgagcgcatc ggcgagatct
     3721 ggctcgatgt gcagcgagag agcctcgggc ccgccatcgc cttcgacgac gactaccgcg
     3781 cttattggag ctacatcccg catttcatcc acacgccgtt ctacgtctat gcctatgcct
     3841 tcggcgattg cctggtgaat tcgctctacg ccgtctatca gaacgcggcg ccgggctttg
     3901 cggaaaagta tctcgacatg ctgagcgccg gcggcacgcg gcgtcatcgc gagctcctgg
     3961 ctcccttcgg cctcgacgcc tccgatccgg ccttctggaa gaaaggcctc tcggtgatct
     4021 cggccttcat cgacgaactc gagaagatgg ggtgatcaag ccgatggcac acgtcagaat
     4081 cccctctccc cttgtgggag agggaggggc ccgtgcg
//
LOCUS       DASYNS010000522         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4342042, whole genome shotgun sequence.
ACCESSION   DASYNS010000522 DASYNS010000000
VERSION     DASYNS010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4342042"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VGP50_16135"
     CDS             complement(<1..739)
                     /locus_tag="VGP50_16135"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEV8028964.1"
                     /translation="MSMITIHPAVPPPVPANPLVRVRELVLAGSALIGIFVIGFGTWS
                     AMAPLQSAAVAMGMVVSESSRKTVQHLEGGIIRDILVHDGDTVRAGQVLIRLDDTKAR
                     TTLGALEGQLWDAKAREVRLVSERDGAEQIVFPPDLAPAEAAPALAAALSGQQKIFET
                     RRRLQESKIEAIHERINQVHAEIDGHDAEIAGLHKRTVFLEEEMAGVRQLVAKGLERK
                     PRLLQLERDMAEMEGKIGDTTAQIAKAR"
     gene            complement(755..>1929)
                     /locus_tag="VGP50_16140"
     CDS             complement(755..>1929)
                     /locus_tag="VGP50_16140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8028965.1"
                     /translation="KTHQRAEAALRNAEVIRAMGMTEGVLRLWRHDTAGAQEAQRAAG
                     VRGGTILAISKFVRLLVQTVILGIGAWLVIRQEASAGAMFASAFLLGRALAPAENAIA
                     TWKSLVGARLARRRLEDLVGALPDEARGMTLPRPMGELSVDRVVFVPPGGEEPTLRGI
                     SFDLRPGEVLGVVGPSAAGKSTLARLIAGTWTPTAGKVRLDGAEINVWHDSGGSHHIG
                     YLPQDIELFSGSVRDNIARLGEAGPAAVIEAAKLVGLHELIMRLPKGYNSEIGEAGLR
                     ISGGQRQRIALARAIFGRPSLVVLDEPNASLDHEGEEALHRAIAQLKELGTTIVMIAH
                     RPSVLALADKLLVIRNGMVDAYGSRAEVIAKLNAPGPGRRAVPMPAHADQPQQQIA"
BASE COUNT          286 a          654 c          685 g          304 t
ORIGIN      
        1 gcctcgcctt ggcgatctgc gccgtggtgt cgccaatctt gccctccatc tccgccatgt
       61 cgcgctcgag ctgcaaaagg cgcggcttgc gctcgagacc cttggcaacg agctggcgga
      121 caccggccat ctcttcctcc agaaacaccg tgcgcttgtg caggcccgcg atctcggcgt
      181 catggccatc gatctcggca tggacctggt tgatgcgctc gtggatggct tcgatcttcg
      241 attcctggag ccggcggcgc gtctcgaaga tcttttgctg gccgctcagc gcggccgcca
      301 gcgcgggcgc agcctcggcg ggcgcgagat cgggcgggaa cacgatctgc tcggcaccgt
      361 cgcgctccga gacgagccgt acctcgcgcg ccttggcatc ccagagctgg ccctcgagcg
      421 ccccgagagt ggtgcgcgcc ttggtatcgt cgaggcggat caggacctgc ccggcgcgga
      481 cggtgtcgcc gtcatggacg aggatgtcgc ggatgatgcc gccctcgaga tgctgcacgg
      541 tcttgcggct cgattccgag accaccatgc ccatggccac ggcggcgctc tggagcggcg
      601 ccatcgccga ccaggtgccg aagccgatga cgaagatccc gatgagagcg ctgcccgcca
      661 gcaccagctc gcgcacccgc acgagagggt ttgcgggaac cggcggcggt accgcgggat
      721 ggatggtgat catgctcatg agacgtgtct tcctctaagc gatctgctgc tgcggctggt
      781 cggcatgcgc gggcatcggc acggcgcgcc ggccggggcc cggcgcgttg agcttggcga
      841 tcacttcggc gcggctgcca taggcatcga ccatgccgtt gcggatcacc aagagcttgt
      901 cggcgagggc gagaacgctg gggcgatggg cgatcatgac gatggtggtg ccgagctcct
      961 tgagctgggc gatggcgcga tggagcgcct cctcgccttc gtgatcgagg ctggcattgg
     1021 gctcgtcgag cacgacgagg ctcggccggc cgaagatggc gcgagcgagc gcgatgcgct
     1081 ggcgctggcc gcccgagatg cgcaagccgg cctcgccgat ctcgctgttg tagcccttgg
     1141 gcaggcgcat gatcagttcg tgaaggccga ccagcttggc ggcctcgatg acggcggcgg
     1201 ggccggcctc gccgagacgc gcaatgttgt cgcgcaccga gcccgagaag agctcgatgt
     1261 cctgcggcag gtagccgata tggtgcgagc cgcccgaatc gtgccagacg ttgatctcgg
     1321 cgccgtcgag gcgcaccttg cccgccgtcg gcgtccaggt gccggcgatg agccgcgcga
     1381 gcgtcgactt gccggcggcc gacgggccga caacgccgag cacttcgccc ggccgcagat
     1441 cgaagctgat gccgcgcagc gtcggctcct cgccgcccgg cggaacgaag accacccgat
     1501 ccacggaaag ctcgcccatg ggacgcggca gcgtcatgcc tcgtgcctcg tccggcagtg
     1561 cccccaccag gtcctcgaga cggcgtcggg ccagccgcgc cccgaccagc gacttccagg
     1621 tggcgatggc attctcggcg ggagcgagcg cgcgccccag caggaacgcc gacgcgaaca
     1681 tcgcaccggc gctcgcttcc tgacggatga cgagccaggc gccgattccg aggatcaccg
     1741 tctggacgag gaggcggacg aatttcgaga tggcgaggat cgtgccgccg cgcacgccgg
     1801 ccgcgcgttg cgcctcttgg gcgccggcgg tgtcatgccg ccagaggcgc agcacgcctt
     1861 cggtcatgcc catggcacgg atcacctcgg cattgcgcaa ggccgcttcg gcgcgctgat
     1921 gagtcttgg
//
LOCUS       DASYNS010000523         2297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5121980, whole genome shotgun sequence.
ACCESSION   DASYNS010000523 DASYNS010000000
VERSION     DASYNS010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5121980"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..656)
                     /locus_tag="VGP50_16145"
     CDS             complement(114..656)
                     /locus_tag="VGP50_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028966.1"
                     /translation="MFRDNSLIPAEAVRLAALGLLAEAPRRYGDLALEIRHFTSHVVG
                     PSLDLMGTSLELLRFEGLVAAVDGEGMADNAVLQLTERGRAALDGLLQAQLRAPVNDL
                     NRLALLLKLRFLHHLPAGEQQVQLALMAESLDSERARLEELVRRHGTPGLLAEWLAAD
                     IAMVSERITWLQRRSAAGSQ"
     gene            complement(671..1876)
                     /gene="hemA"
                     /locus_tag="VGP50_16150"
     CDS             complement(671..1876)
                     /gene="hemA"
                     /locus_tag="VGP50_16150"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEV8028967.1"
                     /translation="MDHEQFFKTRLDGLRAEGRYRVFADLERRAGAFPQAFDHHIGRE
                     ITVWSSNDYLGMGQHPAVLAAMHEALDKTGAGTGGTRNISGTTHYHVMLERELAALHA
                     KPAALVFTSGYVANDAALATLAGQTPDCIVFSDALNHASMIEGIRHSGAEKQIFAHND
                     PTDLERRLAAVDPRRPKLVAFESVYSMDGDIAPIGELCDVAERYGAMTYLDEVHAVGL
                     YGKQGGGVAERDGVSHRLTVIQGTLAKAFGLIGGYVAGSAVLIDFLRSFAPGFIFTTS
                     LPPAIAAGATASIRHVRSTPALRERHQERAQRLKRRLAEAELPVMPSPSHVVPVLIGD
                     ARLCKQASDDLLERHRIYVTPINYPTVPRGTERLRLTPSPQHSDEDIERLVGALCDVW
                     SRLPIRRVA"
BASE COUNT          392 a          705 c          825 g          375 t
ORIGIN      
        1 agcttcaggg cgacgggatg taagccgcga acgcgcgaag tgcgttcgtc ggcggcggcg
       61 tcagcgcgaa gcgctcgtcg cgagagccgc accgtgcagg attggatgag gggttattga
      121 ctcccggcgg ccgaccggcg ctggagccag gtgatgcgct cgctcaccat ggcgatgtcg
      181 gcggcgagcc attcggcgag gaggccgggt gtgccgtggc ggcggacgag ctcctcgagg
      241 cgcgctcttt cgctgtcgag tgattccgcc atgagggcga gttgcacctg ctgctccccg
      301 gccggcaaat ggtgaaggaa acgcagcttg aggagcaggg ccaggcgatt gaggtcgttg
      361 acgggagcgc gcagctgcgc ctgcaacagg ccgtcgaggg cggcgcggcc gcgctcggtg
      421 agctggagca ccgcattgtc ggccatgcct tcgccatcga ccgcggcgac gagcccctcg
      481 aagcgcaaga gctcgagcga cgtgcccatg aggtcgaggg aaggcccgac cacatggctc
      541 gtgaagtgcc ggatctcgag ggcgagatcg ccatagcgcc gcggcgcctc ggcgaggagg
      601 cccaaggccg cgaggcgcac cgcctcggcg gggatcagcg aattgtcgcg gaacatcggc
      661 gctctctctc ctaggcgacg cggcggatgg gaagacgcga ccatacgtcg cacagcgctc
      721 ccacgaggcg ctcgatgtcc tcgtcgctgt gctgcgggct cggcgtgagg cgcagccgct
      781 cggtgccgcg cggcaccgtc gggtagttga tcggcgtgac atagatgcga tggcgctcga
      841 gcaggtcgtc cgaggcctgc ttgcagagcc gcgcatcgcc gatcagcacc ggaaccacat
      901 ggctcggcga cggcatcacc ggcagctccg cctcggcgag gcggcgcttc agccgctgcg
      961 cgcgctcctg gtggcgctcg cgcagcgccg gcgtgctccg cacatggcgg atgctggcgg
     1021 tggcgcccgc ggcgatggcg ggcggcagcg aggtcgtgaa gatgaagcca ggcgcgaagc
     1081 tgcgcaggaa atcgatgagc acggccgagc ccgcgacata gccgccgatg aggccgaagg
     1141 ccttggccag cgtgccctgg atgacggtga gccgatggct gacgccgtcg cgctcggcga
     1201 cgccgccgcc ctgcttgccg tagagtccga cggcgtgcac ctcgtcgaga taggtcatgg
     1261 cgccataacg ctcggccacg tcgcagagtt cgccgatggg tgcgatgtcg ccatccattg
     1321 aatagacgga ttcgaaggcg acgagcttcg gccgccgcgg atcgacggca gcgagacgcc
     1381 gctcgagatc cgtcggatcg ttatgggcga agatctgctt ctcggcgccg ctgtggcgga
     1441 tgccctcgat catcgaggcg tggttgaggg cgtcggagaa gacgatgcag tccggtgtct
     1501 ggcctgccag cgtcgcgagc gcagcgtcgt tggcgacata gcctgaggtg aagacgagcg
     1561 ccgccggctt ggcatggagg gccgccagct cgcgctccag catcacatgg tagtgggtcg
     1621 tgccggagat gttgcgcgtg ccgccggtgc cagcgccggt cttgtcgaga gcctcatgca
     1681 tggccgccag cacggccgga tgctgcccca tgccgagata gtcgttggag gaccagacgg
     1741 tgatctcgcg gccgatgtgg tggtcgaagg cctgggggaa agcgccggcg cggcgctcga
     1801 gatccgcgaa gacgcgatag cgcccttcgg cccggaggcc gtcgagtcgc gtcttgaaaa
     1861 attgctcatg atccatggct ttgcttcccg cctgctccat gaaatttaca tgaaaccgga
     1921 actatttata cgctctccgt tccacccatc ccatcaaacc cgaaccagac caaggagaaa
     1981 agaactgctt cacaactttc gagtcttaca attgagtaaa gttgtcaatg tgtaaatttt
     2041 tgacaaaccg atcgttagca cggcaaagct tgtcaacggg tcgggcggct ccagcgtcct
     2101 ttcgggcgcc tcttcccatt cgggaagtga ccatacccta taccagggca gggtgaccgg
     2161 cgcggccgag tgaagctgcc gtgtcgtcga gtgcctggcc gaagcggcgc agcatttcgg
     2221 cgatctcggc cgcggtgatc accagcggcg ggcagagggc gatgttgtcc tggagggcgc
     2281 ggatgatcag gccgtgc
//
LOCUS       DASYNS010000524         8597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_755479, whole genome shotgun sequence.
ACCESSION   DASYNS010000524 DASYNS010000000
VERSION     DASYNS010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755479"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..928
                     /locus_tag="VGP50_16155"
     CDS             149..928
                     /locus_tag="VGP50_16155"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV8028968.1"
                     /translation="MTLLKRSDHLWRLVATGIAFAAMGVGGVVLALVVIPLALLPMRD
                     RDVRARRAQAIIRTSFRFYVAMLRRFGVISLEVVGEARLANCRGSLIIANHPTLLDVV
                     LIMALVPNAQCVVKHQLWRSPFLGPVVRIAGYIRNDEDGERFIENCRRALGRGANLII
                     FPEGTRSVPGRPPHFQRGFAHVATLTGASLQLIAITCDPITLVKGQPWYAIPERRPAF
                     RVEIAERMDAKPFLELGPRSLGARKLVSHLESYYGGKLKHG"
     gene            921..1184
                     /locus_tag="VGP50_16160"
     CDS             921..1184
                     /locus_tag="VGP50_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEV8028969.1"
                     /translation="MDDLELELKRLIVRALSLEDITPDAIASDEPLFGDGLGLDSIDG
                     LELGMAIRKTYGLKIDAKNEEVRKIFFCVRSIAQFIAAQRGHA"
     gene            1184..1438
                     /locus_tag="VGP50_16165"
     CDS             1184..1438
                     /locus_tag="VGP50_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV8028970.1"
                     /translation="MVSKTDILATLSGYLQDMFEVPADKITLGARLFDDLDLDSIDAV
                     DLVVKLQEYTGKKIAPAEFKSVRTIGDVVEQVYAQLSQRA"
     gene            1413..1994
                     /locus_tag="VGP50_16170"
     CDS             1413..1994
                     /locus_tag="VGP50_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028971.1"
                     /translation="MPSFLRGRSLRLAIGLVALAYPFLVYFGLRLASPAALGVVLLAV
                     LALRLVVDRQDSARRSFAPAFWIAAAVALLLVALSPLAGLQSYPILLSLGFAGLFGHS
                     LWQPPTVIERIARLRDPNLPPSAVAYTRKVTAVWLGFFLANAAISTATAIGGSLELWT
                     LYNGFISYVIMGVLFISEWILRGYLRVAHRDTA"
     gene            1991..3685
                     /locus_tag="VGP50_16175"
     CDS             1991..3685
                     /locus_tag="VGP50_16175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV8028972.1"
                     /translation="MSALGLAQLLNGQRPSDFPVAMTGGRVLTLGRLRADVARNAAAL
                     RGARRALLVCEDSYRFLTGLLALLHIGADIVLPPNAQSGTLRMIGDSFDLLVTDVSRA
                     DVARGFVIESTDAEAAPFMLEVERGRLDFFTSGSTGDMKRVEKTTRLLEREAQILETL
                     WGGQLANAQVFATVSHQHIFGLTFKLLWPLLAGRCFCTETHGAWEALLAELPPGAVIV
                     SSPAHLTRIGGLTPLPPSRRPRMILTAGAPLPPEAAAETQEILGTAPTEIFGSTETGA
                     IAWRTVTTGPSFWQALPGIEIGSHGDGLLRVRSPLIGGDGWCDLADRIVIADDGRFRF
                     EGRADRILKIEGKRVSLQQLEREMAALPWIEAAAVAPLAGPRSVLGAVAVLSAVGKGE
                     LARLGKFRFERLLRRALANTQDGAALPRRWRFVEQLPMDGMGKRRTADIAALLAEAPM
                     SVTEPVIRDVRRSGGGVDLSLSVPKTLLHLSGHFPGFAVLPGVVQLDWAIRYGRQYLG
                     LGSAAAKTVQVKFRKPIRPDRPFDLRLDYSVEQRRLSFECRDEDGVYSSGQVVFDA"
     gene            3682..5346
                     /locus_tag="VGP50_16180"
     CDS             3682..5346
                     /locus_tag="VGP50_16180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV8028973.1"
                     /translation="MIFKPCAVVPSRNHAAALPVILQRLREAGLPVFLIDDASDVPAR
                     DTIAALAAPRRGIIVHRFETNQGKGGAVIKGFELADAAGFTHAIQVDADGQHDLGALP
                     SLLSLGAAHPDALVLGTPVYDDTMPLARKLGRWITHVWVAIETLSPRIIDTMCGFRIY
                     PLAPVMALLAEERIGRRMDFDIDIAVRLIWRGVPLSTLPVRVTYPPGNTSNFDALRDN
                     WRISRMHARLVLEGLGRLLGIRRRPAGEEANHWSGLAERGAYWGLRVLAAAYRLTGRY
                     GCMAAMLPVALYFHVTGREQRRASRLFLERAYAALGDTHGPRWSDGLRHSLGFAQRTV
                     DTFIGWVGGVDRNSIEVVDQAEIDRVIAGGRGVMLIVSHLGNVELSRASLDDAARARI
                     TLLVHTRHAENYARLLRRFRPEAAIDTFQVTEVNPGTVIALKEVVERGGWVAIAGDRT
                     PVGGGERVSRAKFLGHEAAFPQGPYILAHLLECPVYLMFCLRENGRHRLYFERFAERV
                     VLPRGDKEAALAALAGRYASRLEAYCLRDPFQWYNFFDFWAEAGAR"
     gene            5343..5750
                     /locus_tag="VGP50_16185"
     CDS             5343..5750
                     /locus_tag="VGP50_16185"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV8028974.1"
                     /translation="MISAEVIVKAQFYDLDPMQIVWHGNYVRFLEQARCALLDKIGFG
                     YREMAETGYLWPVVDLRIKYVRPVRFGQEVRVTATMAEYEYRLKIDYRLADLATGEVL
                     TKAHTIQVAVDAATNELCFQSPAVLYAKVEGHR"
     gene            5747..6358
                     /locus_tag="VGP50_16190"
     CDS             5747..6358
                     /locus_tag="VGP50_16190"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEV8028975.1"
                     /translation="MMRALVALAILAVTVAAAPPRQVHPLGNGEVIAGRFVQERHLSG
                     LAAPLRSEGRFVLAAGRGLIWRGEKPFATVTVLTPAGVTQSVDGKEVQRLPASRVPFL
                     SRFYQMLSGALAGDWTAMEHDFTIDRQEGEPRWTIVLHPRSSDDPASAQLQSITVTGD
                     TLVEGVEIHRANGDWEHLTFLDQVLSSAPLSAEDAQLLSAPPP"
     gene            6355..>8597
                     /locus_tag="VGP50_16195"
     CDS             6355..>8597
                     /locus_tag="VGP50_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028976.1"
                     /translation="MKIAGVIWLFIVAVATVSLLVRLHDGVQFQSDLFALLPQEDRDP
                     AVQRAKEKAGALLGQRIILLVGDEDRANARSAGAELAAALAASDVASAVTYRLPPDGL
                     RRIAEIFFPYRRGLLTAADRSRLEEGRGREIVARALASVYGPAGFADAALLQRDPFLL
                     MATFLAALPTPLPRLAPDDGVLSVRDDTKTYVLVAAQLSGDTFALAAQDHFIQILGAA
                     EEKLRSRLPDVEILRFGTVFYAHAGAAAAVAETSDISIVSLIGTVILVLAVFRAFRPL
                     WLTMLAIGVGVVCAFAASLWIFGTLHVAALLLGVSLIGISLDYCLQYLSARFDATANT
                     PWRRLSRVLPGISLGIATTLIGYVTLLVAPFPGLRQVAVFSAVGLVASFITVILWLPA
                     LDASQPFRHGRRLLVAADWLWSFWGAPRYRRPRLVLVGLAVLLAVAGASVFTVDDDVR
                     RLQTISQDLKRQEEAIRRLTGLSGATEFLLLRAADGEAALETEEVLLVRLDEARQEGA
                     LKGYQALAQVVPSIARQRADGALVRDKLNAPFLAEYYAQLGMPADAMIEEPGDRFLVP
                     AAIPDDSPLAALRQLAIADGPAGAMHLVLLSGVADRDEVKRLAQSVPGVRLIDPAGDV
                     TRLLGDYRRRAVLLLGLSLLFMMPVVIWRYGWRGSLRVLVAPTVAVVLAPLLVALAGI
                     PFTFFGAMALVLVLSIGFDYAVFCKETDPAQRPVTMLGVWLAMVTTLLSFGLLAFSRV
                     FAVQAFGA"
BASE COUNT         1204 a         2921 c         2809 g         1663 t
ORIGIN      
        1 ggggacctgc tgctgtccta cgcgccagga ccgcgggacg cgccggcgtg cccggccacg
       61 gaccaggcgc tcgatttcct gcgcttcctg ctgtcgaatg agagcgagag gacctcgtcc
      121 ggaggctcga tcgaatggcg ccggcaccgt gaccctgctt aagcgatccg accatctgtg
      181 gcgcctcgtc gccaccggga tcgccttcgc cgccatgggc gtgggcggcg tcgttctggc
      241 gctcgtcgtc attccgctgg cgctcctgcc aatgcgcgat cgcgacgtcc gcgctcgccg
      301 cgcccaagcc atcatccgca ccagcttccg tttctatgtg gcgatgcttc gccgattcgg
      361 ggtgatcagc ctcgaggtgg tcggcgaggc gcggctggcg aattgccgcg gcagccttat
      421 catcgccaac catccgacgc tgctcgacgt cgtcctgatc atggccctgg tgcccaacgc
      481 gcaatgcgtc gtgaagcacc agctctggcg cagtccgttc ctcggcccgg tcgtgaggat
      541 cgcaggctac atccgcaacg acgaggatgg cgagcgcttc atcgagaact gccggcgcgc
      601 gctcggccgt ggcgccaatc tcatcatatt cccggagggc acgcgctcgg tgccgggtcg
      661 cccgccgcat ttccagcgcg gcttcgccca tgtggcgacg ctcaccggtg ccagccttca
      721 gctcatcgcc atcacctgcg atcccatcac gctcgtcaag gggcagccct ggtacgccat
      781 tcccgagcgg cgtcccgcct ttcgcgtcga gatcgccgag cgtatggacg cgaagccgtt
      841 tctcgaactc ggcccgcgct cgctcggcgc gcgcaagctc gtttcgcacc tcgaatctta
      901 ctatggcggc aagttgaagc atggatgatc ttgaattgga gctcaaacgg ctgatcgtgc
      961 gcgcgctcag cctggaggac ataacgccgg acgcgatcgc cagcgacgaa ccgttgttcg
     1021 gcgacggcct cggcctcgac tccatcgacg gcctcgagct cggcatggcg atccgcaaga
     1081 cctatggtct caagatcgac gccaagaacg aagaggtgcg gaagatcttc ttctgcgtgc
     1141 gcagcatcgc gcagttcatt gccgcccagc gagggcatgc ctgatggtct cgaaaaccga
     1201 catcctggcg acactcagcg gctatcttca ggacatgttc gaagtgccgg cggacaaaat
     1261 cacgctcggc gcgcgattgt tcgacgatct cgatctcgac agcatcgatg cggtcgatct
     1321 ggtggtgaag ctgcaagaat acacgggaaa gaagatcgct cccgcggagt tcaagtcggt
     1381 ccgcacgatc ggcgacgtcg tagagcaagt gtatgcccag ctttctcaga gggcgtagcc
     1441 ttcgtctcgc catcggcctc gtggccctcg cttatccgtt cctggtctat tttggcctgc
     1501 gcctcgcctc gccggcggca ctcggcgtcg tcttgctggc ggttctggcg ttgaggctcg
     1561 tcgtcgaccg gcaggacagc gctcgccgtt ccttcgctcc tgccttctgg attgcggccg
     1621 ccgtggcgtt gttgctggtc gcgctctcgc cgctcgccgg ccttcaatcc tacccgatcc
     1681 tgctcagcct cggcttcgcc ggcctctttg ggcattcgct gtggcagccg ccgacggtga
     1741 tcgagcggat cgcgcgcctg cgcgacccga atctgccgcc atcggcggtc gcctatacgc
     1801 gcaaagtgac ggccgtttgg ctcggattct tccttgccaa tgcggcaatc tcaacggcga
     1861 ccgcgatcgg cggcagcctc gagctgtgga cgctttacaa cggattcatc tcctacgtca
     1921 tcatgggtgt gctcttcatc agcgagtgga tcctgcgcgg ctacctgcgc gttgctcatc
     1981 gagacaccgc atgagcgcgc ttggtctcgc gcagctcctg aacgggcagc gcccgagcga
     2041 cttcccggtt gccatgaccg gcggtcgagt gctcacgctg ggccggcttc gcgccgatgt
     2101 ggcccgcaac gccgccgctt tacgcggcgc gaggcgcgcg ctcctcgtct gtgaggacag
     2161 ctatcgcttc ctcacgggat tgctggcgct gctccatatc ggcgccgata tcgtgttgcc
     2221 gcccaatgcg cagagcggca cgttgcgcat gatcggcgac agcttcgatc tcctggtcac
     2281 cgatgtgagt cgggcggatg tcgcgcgcgg gttcgtgatc gaatcaaccg atgccgaagc
     2341 cgcccctttc atgctcgagg tcgagcgcgg ccgccttgat ttttttacct cgggctcgac
     2401 cggcgacatg aaacgcgtgg agaagacgac gcggcttctc gagcgcgagg cgcagattct
     2461 cgagaccctg tggggtgggc agctggcgaa cgcccaggtt tttgccaccg tctcgcatca
     2521 gcatatcttt ggcctcacct tcaagctgct ctggccgctt ctcgctgggc gatgcttttg
     2581 caccgagacc cacggcgcat gggaggcgct gcttgccgag ctgccgcccg gcgccgtgat
     2641 cgtgtcgagc ccggcgcatc tgacccgcat cggtgggctt acgcctttgc cgccgagccg
     2701 acggccgcgc atgatcttga cggccggagc tcctctgccg cccgaggctg cggccgagac
     2761 gcaggagatt ctcggcacgg cgccaaccga aatcttcggc agcacggaaa cgggggcgat
     2821 cgcctggcgg acagtcacga caggcccgag cttctggcaa gcgttgcccg gcatcgagat
     2881 cggctctcat ggcgacgggt tgctgcgcgt gcgctcgcct ctcatcggtg gcgatggctg
     2941 gtgtgacctc gcggatcgca tcgtcattgc cgatgatggc cgcttccgct tcgaggggcg
     3001 tgccgaccgg atcctcaaga tcgaaggcaa gcgggtgagc ttgcagcagc tcgagcggga
     3061 gatggcggcg ctgccatgga tcgaagccgc cgcggtggcg ccgctcgccg gtccgcgcag
     3121 cgtgctcggc gcggttgcag tgctctcggc ggtgggaaaa ggcgagctcg ctcggctcgg
     3181 caaatttcgc ttcgagcgtc tgctgcggcg cgcgctcgcg aatacccagg acggcgccgc
     3241 gctgccgcgg cgctggcgtt tcgtcgaaca gctgccgatg gacggcatgg gcaagcgccg
     3301 gacggccgat atcgccgcgc tgctcgccga ggctccgatg agcgtgacgg agcccgtgat
     3361 tcgcgacgtc cggcgttccg gcggcggggt cgatctgtct ctctccgtgc cgaagactct
     3421 gctccacctg agcggccatt tcccgggctt cgccgttctg cccggcgtcg ttcagctcga
     3481 ttgggctatc cgttacggcc ggcaatatct tggcctgggc agcgcggcgg cgaagaccgt
     3541 gcaagtgaag tttcgcaagc ccatccggcc cgatcggccc ttcgacctgc gcctcgacta
     3601 cagcgtcgag cagcggcggc tctccttcga gtgccgggat gaggacggcg tctactcctc
     3661 aggccaggtc gtcttcgacg catgatcttc aagccttgcg ccgtcgtgcc gagccgcaat
     3721 catgccgcgg cacttccggt catcctccag cggctccgcg aagccggctt gccggtcttc
     3781 ctgatcgatg acgcgagcga tgtcccggcc agagacacca tcgccgccct cgccgcgccg
     3841 cggcgcggca tcatcgtcca tcgcttcgag acgaaccagg gaaagggcgg tgccgtcatc
     3901 aagggtttcg agcttgccga tgccgccggc ttcacccacg cgatccaggt cgatgccgac
     3961 gggcaacacg atctcggggc gctgccaagc ctgctgtcgc tcggcgccgc gcatcccgac
     4021 gctctcgtcc tcgggacgcc ggtctatgac gacaccatgc cgctcgcgcg aaagctcggc
     4081 cgctggatca cgcatgtctg ggtcgcgatc gagactctgt cgccgcggat catcgatacg
     4141 atgtgcggct ttcgcatcta tccgctcgcg ccggtcatgg cgctgctcgc cgaggagcgc
     4201 atcggccggc gcatggactt cgacatcgac atcgccgttc gcctcatctg gcgcggcgtg
     4261 cccctgagca cgttgccggt cagggtgact tatcctcccg gaaatacctc gaatttcgat
     4321 gccctccgcg acaactggcg catcagcagg atgcatgcgc ggctggttct ggagggactc
     4381 ggtcgcttgc tcgggatccg gcggcggccg gcgggcgagg aggcgaacca ttggtcgggc
     4441 ttggcggagc gcggcgccta ctggggcctg cgcgtccttg ccgctgccta ccggttgacg
     4501 ggtcgctacg gttgcatggc ggcgatgctg ccggtggcgc tctatttcca cgtcaccggg
     4561 cgcgagcagc gccgggcctc gcggctcttt ctcgaacgcg cctatgcggc gctcggcgac
     4621 acacacggac cgcgctggag tgacgggctg cggcactcgc tcggcttcgc gcagcggaca
     4681 gtcgatacgt tcatcggctg ggtcggtggc gtcgacagaa acagcatcga ggtggtggat
     4741 caggccgaga tcgatcgggt gatcgccggc gggcgcggcg tcatgctcat cgtctcgcat
     4801 ctcggcaacg tcgagttgtc ccgcgcttcg ctcgatgacg ccgcgcgcgc acgcatcacg
     4861 ctgctggtgc acacgcgtca cgccgagaac tacgcccggc tgctgcgccg ctttcggccg
     4921 gaggcggcca tcgacacgtt tcaagtgacc gaggtcaatc cggggacggt catcgctctg
     4981 aaggaagtgg tggagcgggg tggctgggtc gcgattgccg gcgaccgcac gccggtcggc
     5041 ggcggcgagc gggtgagccg cgccaagttt ctcggccatg aggccgcttt cccgcagggc
     5101 ccctatattc tcgcccatct cctcgaatgc ccggtctatc tcatgttctg cctgcgcgag
     5161 aacggccgcc atcgccttta tttcgagcgt ttcgccgagc gcgtcgtcct gccgcgcggc
     5221 gacaaggaag cggcgcttgc cgcgctcgcc ggtcgatacg ccagccgcct cgaggcctat
     5281 tgcctgcgcg atcccttcca gtggtacaat ttcttcgatt tctgggcgga ggccggcgcg
     5341 cgatgatctc cgccgaggtg atcgtcaaag ctcaattcta tgatctcgac ccgatgcaga
     5401 tcgtctggca cggcaactat gtgcgcttcc tcgagcaggc gcgctgcgcg cttctcgaca
     5461 agatcggatt cggctatcgc gagatggcgg agacgggcta tctctggccc gtggtcgatc
     5521 tgcgcatcaa atacgtgcgt ccggtccgct tcggccagga ggttagggtg accgcgacca
     5581 tggccgaata tgaataccga ctcaagatcg actatcgtct cgccgatctc gcgacaggag
     5641 aggtgctgac gaaagcgcat acgatccagg tcgctgtcga tgccgccacc aacgagctgt
     5701 gcttccaatc gcccgccgtg ctctatgcga aggtggaagg ccaccgatga tgcgcgcgct
     5761 cgtggcgttg gccattctgg cggtcacggt tgcggcggct ccgccgcgcc aagtgcatcc
     5821 gctcgggaat ggcgaggtca tcgccggccg cttcgtccag gagcgtcatt tgtcgggatt
     5881 ggcggcgccg ctgcgcagcg aagggcgctt cgtgctcgcg gccggccgcg gcctgatctg
     5941 gcgcggcgag aagccgtttg cgacggtgac cgtcctcaca cccgcgggcg tcacgcagag
     6001 cgtcgacggc aaggaggtgc agcggctgcc ggcgtcgcgc gtccccttcc tctcgcgctt
     6061 ctaccagatg ctgagcggcg cattggccgg cgactggacg gcaatggagc atgatttcac
     6121 gatcgatcga caggagggcg agccgcgctg gaccatcgtc ctgcatccgc ggtcatcgga
     6181 cgatcctgcg agcgcccagc tccagtcgat caccgtcacg ggcgacacgc tcgtcgaagg
     6241 ggtcgagatc caccgtgcca atggcgactg ggagcatctg accttcctcg accaggtgtt
     6301 gtcctccgcg ccgctttccg ccgaggacgc gcagctcctc tcggcgcctc cgccgtgaag
     6361 atcgctggtg tgatctggct gttcatcgtg gccgtagcga ccgtctctct gctcgtccga
     6421 ttgcatgacg gcgtgcaatt tcagagcgat ctcttcgccc tcttgcccca ggaggatcgc
     6481 gatcccgcgg tccagcgggc gaaagagaag gccggggccc tgctgggcca gcgcatcatt
     6541 cttctcgtcg gcgacgagga ccgcgccaat gcgcgatcgg ctggtgccga gcttgcggcc
     6601 gcgctggccg cttcggatgt cgcgtccgcg gtcacctata ggctgccgcc cgacgggctg
     6661 cgccggatcg ccgaaatctt ctttccctat cggcgcggtc tgctgaccgc ggccgatcga
     6721 agccgcctcg aagagggccg cggccgcgag atcgttgcgc gggcactggc cagcgtctat
     6781 ggccccgcgg gtttcgccga cgctgccctg ctgcagcgcg atccgttcct cctgatggcg
     6841 actttccttg ccgcactgcc gacgccgctg ccgcgattgg cgcccgatga cggcgtcttg
     6901 tcggtgcgcg acgacacgaa gacctatgtc ctggtggcgg cgcagctcag cggcgatacc
     6961 ttcgcgctcg cggcgcagga ccatttcatc cagatcctcg gcgcggccga ggagaaattg
     7021 cgatcccgcc ttccggatgt cgagatcctg cgcttcggca cagtgttcta tgcccatgcc
     7081 ggcgcggccg cggcggtcgc ggagacctcg gacatcagca tcgtctcgct gatcggaacg
     7141 gtcattctcg ttctcgccgt tttccgcgcg ttccggccgc tctggctcac catgctcgcc
     7201 atcggcgtcg gcgtggtctg cgccttcgcc gcgagcctgt ggatcttcgg cacgttgcat
     7261 gtggcggcgc tgcttctggg ggtgagcctc atcggcatct cgctcgacta ctgcctccaa
     7321 tatctttccg cccgcttcga tgccacggcc aacacgccct ggcggcgcct ctcgcgcgtg
     7381 ttgcccggca tcagccttgg catcgcgacg accttgatcg gctatgtcac gctgctggtc
     7441 gcacctttcc ccgggctgcg ccaggtggcg gtcttctcgg ccgtcggcct cgtcgcctcc
     7501 ttcataacgg tgatcctatg gctgccggcg ctcgatgcca gccagccctt tcgccatgga
     7561 cggagattgc tcgtcgccgc cgactggctc tggtccttct ggggagcgcc gcgttatcgg
     7621 cgtccgcgcc tggtgctggt cggcctcgcc gtgctgctcg cggtcgccgg ggcctctgtc
     7681 ttcaccgtcg atgacgatgt gcgccgcctc cagacgatct cgcaggacct gaagcgacag
     7741 gaggaggcga ttcgccgctt gaccggcctc tccggcgcga ccgagttcct gctccttcgc
     7801 gcggccgacg gcgaagccgc gctcgagacc gaagaggttt tgctcgtgcg gctcgacgaa
     7861 gcgaggcagg agggcgcact caagggctat caagcgctgg cgcaagtggt cccttcgatc
     7921 gcgcggcagc gtgccgacgg tgctctcgtg cgcgacaagc tgaacgcgcc cttccttgcc
     7981 gagtactatg cgcagctcgg catgcctgcg gatgcgatga tcgaagagcc gggcgatcgt
     8041 tttctcgtgc ccgccgctat cccggacgat tcaccgctcg cggcgctgcg ccagctcgct
     8101 atcgccgacg gccccgccgg agccatgcat ctggtcttgc tcagtggcgt ggcggatcgc
     8161 gacgaggtga agcggctcgc ccagtccgtt ccgggcgtcc gcctcatcga cccggcgggc
     8221 gatgtcacgc ggctcctcgg cgactaccgg cgccgcgccg tcctgctgct cggcctttcg
     8281 ctgctcttca tgatgccagt ggtgatctgg cgctacggct ggcgcggcag cctgcgcgtc
     8341 ctggtggcgc cgaccgtcgc ggttgttctc gcgcccctgc tcgtggccct tgcggggata
     8401 cccttcacct ttttcggggc gatggcgctc gtgctggtgc tgtcgatcgg tttcgactac
     8461 gcggtcttct gcaaggaaac cgacccggcg cagcgtccgg tcacgatgct cggcgtctgg
     8521 ctggcgatgg tcacgacgct cctgtccttc gggttgctgg ccttcagccg cgtcttcgca
     8581 gtccaggcct tcggcgc
//
LOCUS       DASYNS010000525         8960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4031434, whole genome shotgun sequence.
ACCESSION   DASYNS010000525 DASYNS010000000
VERSION     DASYNS010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8960
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4031434"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..305)
                     /locus_tag="VGP50_16200"
                     /pseudo
     CDS             complement(1..305)
                     /locus_tag="VGP50_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008033944.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
     gene            complement(700..1020)
                     /locus_tag="VGP50_16205"
     CDS             complement(700..1020)
                     /locus_tag="VGP50_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV8028977.1"
                     /translation="MAILREIRRRSRLIVGPILGISLCGYFAFHLVEGDRGLVAWARL
                     SQQVRDARGTLAKAEAERVILERRVQLLRPEHLDRDMLDERARSQLNLVGPNETVVVT
                     PTHQ"
     gene            complement(1234..5184)
                     /locus_tag="VGP50_16210"
     CDS             complement(1234..5184)
                     /locus_tag="VGP50_16210"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8028978.1"
                     /translation="MTKGKRGAHTCLAGGGEMGALMRAIDWSTTPLGPVESWSQALRA
                     IVSMMLANRFPMLLWWGPDYVQLYNDAYAPVPGTKHPKSMGQRACDCWPEIWHILRPL
                     IDTPFDGGPATWMEDILLEVNRHGFLEETHFTIAYSPVPDEAAPRGIGGVLATVHEIT
                     EKVVGERRIVALRDLGARAAEAKTAEQACVIAGRTLANHAKDIPFALLYLIDRDGKRA
                     RLAWSAGIDRGEAASPLVVELDDAADPACGWPLAEAMRREEMVSVEDLAARFGAVPPG
                     PWSDPPHAAVVVPVRSNKVHELAALLVAGVSPRLRLDELYRSFLDLVAAQVATAIANA
                     RAYEEERKRAEALAEIDRAKTAFFSNVSHEFRTPLTLMLGPLEDALAAPAESLPVHRD
                     DLALVHRNSLRLLRLVNTLLDFSRIEAGRVQASFEPLDLAAATADLASVFRAATDKAG
                     LSLTVDCPPLGEPAWVDRDMWEKIVLNLISNAFKFTLEGGIAVRLRRAGASAVLEVED
                     TGTGIPETEIPRLFDRFYRVEGARGRTQEGTGIGLALVQELVRLHGGAVRAESTHGKG
                     SRFIVSIPLGTAHLPPERLRAERTLASTALGAQPYVKEALRWLPDEGRSEEPGIERPL
                     LSEALPAASVDQKSGRATILLADDNADMRDYVRHLLSARYDVRAVADGAQALAALEAR
                     LPDLLLSDIMMPRMSGFELLRAVRSDARLARLPVILLSARAGEEASIEGLEAGADDYL
                     IKPFSARELMARVAANLEMARVRREAAAMSEHRFRFLAEAMPQKIFTTAADGLVDYCN
                     RQWLEFAGLPLEEIMGLGWARIIHPDDNAENVVRWRHSLATGEPFQYAHRFRRRDGAY
                     RWHLTRVHPVREEDGAIAMWIGSSTDIHEQKETEAAYRDLSDTLERKIDERSRALEAE
                     VAERQKVEAALQQAQRLESLGQLTGGVAHDFNNLLTVVLGQIESIMLVAKEDPRILRM
                     AASAQRAAERGAQLTSQLLAFSRRQQLQPRAIAMDRVIVAVGELVRRVISEAIALEVS
                     AAPGLWPAHLDPAQFEAAILNLTINARDAMPKGGRLTIRARNETAGEIEARRLDVAPG
                     PYIVVDVTDTGAGMAPEVRRRAFEPFFTTKDIGKGTGLGLAQIYGFVKQSGGTATIES
                     ALGRGTTVSLYLPKAEAAVIDDEEEEASPDGGASVPGQGKTILVVDDQPAVREAIEAP
                     LARLGYRIIEAADGLAARKLLESDLPIDLLLTDVVMPHGVSGLDLVDEARRLRRDLKI
                     VLVSGYLREFGGRAGGLSGVIFLEKPFKQTDLANAVATALGEGRKPRSRAAPAA"
     gene            complement(5314..6153)
                     /gene="kdsA"
                     /locus_tag="VGP50_16215"
     CDS             complement(5314..6153)
                     /gene="kdsA"
                     /locus_tag="VGP50_16215"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEV8028979.1"
                     /translation="MHHRHVHIGNLTLGNDRPVALIAGPCVLESRQHALEMSHALTEL
                     TGKLGLGLIYKTSFDKANRTSIAAERGLGLEASLPILAEVRETYGCPVLTDVHSPEQC
                     ARVAEAVDVLQIPAFLCRQTDLLVAAAKTGRAINVKKGQFLAPWDMKNVAHKIAVAGN
                     ENILLCERGVSFGYNTLVNDMRALPIMAETGYPVVFDATHSVQQPGGKGDSTGGERRF
                     VPILARAACAIGVAAVFMETHQDPDHAPSDGPNMVRLQDLPALLEQLIAFDRLAKAPA
                     ILQ"
     gene            complement(6350..7981)
                     /locus_tag="VGP50_16220"
     CDS             complement(6350..7981)
                     /locus_tag="VGP50_16220"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV8028980.1"
                     /translation="MARFIFITGGVVSSLGKGLSAASLGALLQARRFKVRLRKLDPYL
                     NVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERYTGVAARRSDSVTTGRIYSTVIGRE
                     RRGEYLGATVQVIPHVTDAIKEFIQADLDGDEDFVLCEIGGTVGDIESLPFLEAIRQL
                     GNDLGRERTLYVHLTLVPWIPSAGELKTKPTQHSVKELLALGIQPDILLCRCDRPVPE
                     SQRRKIALFCNVNPARVIPALDVDTIYEVPNAYHAEGFDREVCAHFGIDMAEPDLGRW
                     REIVTRIRKPEGVVNIAVVGKYTHLLDSYKSLAEALTHGGIANQVRVNLNWLDSEIFE
                     QPDAVQQLEGVHGILVPGGFGERGSEGKIEAARFAREKHVPYFGICFGMQMAVIEAAR
                     HLAGLPGAGSTEFGPCEHPVVGLMTEWMRGNTLERRRAGGDLGGSMRLGAYEAMLAPG
                     SQVSEAYGGAQRVSERHRHRYEVNVNYKARLERAGLLFSGMSPDGELPETVELPGHPW
                     FIGVQYHPELKSKPFEPHPLFTSFIRAARDQSRLV"
     gene            complement(8122..8469)
                     /gene="secG"
                     /locus_tag="VGP50_16225"
     CDS             complement(8122..8469)
                     /gene="secG"
                     /locus_tag="VGP50_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV8028981.1"
                     /translation="MTTVILVVHLMVAVSLVAVILLQKSEGGALGMGGGGMSGFMTGR
                     STANLLTRTTAILAGAFFVTSIALAYLAGGASSAPRSVIDQPPAATEPAAPSTPEPAA
                     PAPTNEPGAPAAK"
     gene            complement(8587..>8960)
                     /locus_tag="VGP50_16230"
     CDS             complement(8587..>8960)
                     /locus_tag="VGP50_16230"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV8028982.1"
                     /translation="LCIGETAEERDAGQTLAVVARQLGGSLPQGLGAAELVIAYEPIW
                     AIGTGRTPTPADIAGVHAHIRAALGKRVADAAAVRILYGGSVKPANARALLTAPEVDG
                     ALVGGASLVAEDFWAIAKAVE"
BASE COUNT         1421 a         3076 c         3034 g         1429 t
ORIGIN      
        1 ccggtgagct cggccatgac gcccttcgga tccatgccgg cggcgagcat gtgcccgtgg
       61 tcgcgataag aggtgatgac cgtgtcgccc ggatcgaccg ccgacatcac gccgacgacg
      121 accgcttcct cgccgatata gagatggcag aagccgccga tcaggcccat gccatacatc
      181 tgaccggcct actcctcgaa gcggcggatc agcagcatct cgcgatagag cttcagcagc
      241 tcggcggccg cgacttcggg ctcctcacgg cccactgcct tgggcttgcg cttcgccaac
      301 gccatgggga ggattcctct gccggaaacg agggggacgg gcgagccgcc ggatcggcgc
      361 taacatggcc cggagaagcg ccaaaagcaa gccggccagc cggtaaaata ttgttttgca
      421 acgcaaaatg aaacttaacc gaatcccggt taagaggatt gaaatggccc aggaacgacg
      481 ccgaccatcg tattccctct ccgcccctca ggggcggaga gggctagggt gaggtggggc
      541 gcgccgcgcc tgagcgcatg gaaccgcctc cgctctcgac gcttttcggc gagatctcgc
      601 gcgagcaacg cgacacaagc cccgggcctc atgggcgcgg ccccacctca ccccgaccct
      661 ctccgcccct gaggggcgga gagggagtgt ccgttacgcc tattgatgcg tcggcgtgac
      721 gacgacggtc tcgttggggc cgacgaggtt gagctggctc ctggcgcgct cgtcgagcat
      781 gtcgcggtcg agatgctccg gccgcagcag ctggacccgc cgctcgagga tcacgcgctc
      841 cgcttcggcc ttggccagcg tcccgcgtgc gtcgcgcacc tgctgcgaga ggcgggccca
      901 ggcgacgagg ccgcggtcgc cctcgaccag gtgaaaggcg aaatagccgc acaagctgat
      961 cccgaggatg gggccgacga tgagcctgga acgccgccgg atctcgcgaa ggatcgccat
     1021 ggcgtgagcg aatcacggcc gagtcgcccc gtcaatcgca gcgagtcgcc gcccctcgcc
     1081 ctgagtcgat ttgccgcagc cgcaagccct tcgagagccc aagctaaaga ttgaccacag
     1141 aggcacagag gacagaaaaa atggcgcggt tcgcgcgcca gattcgattc ttctccgtgc
     1201 ctctgtgcct ctgtggtcaa tctttctttt ctctcacgct gccggcgctg cccggctccg
     1261 cggcttgcga ccctcgccga gtgccgtcgc cacggcgttg gcgaggtcgg tctgcttgaa
     1321 cggcttttcg aggaagatca cgccggacaa gccgccggcg cggccgccga actcgcggag
     1381 atatcccgag acgagcacga tcttgaggtc ccggcgaagc cggcgcgcct catcgacgag
     1441 gtcgaggccg ctcacgccat gcggcatgac gacatcggtc agcagcagat cgatcggcag
     1501 gtcgctctcg aggagcttgc gcgcggcgag gccgtcggcc gcctcgataa tgcgatagcc
     1561 gaggcgcgcc agcggggcct cgatcgcctc gcgcaccgcg ggctggtcgt cgaccacgag
     1621 gatcgtcttg ccctggcccg gcacgcttgc gccgccgtcc ggcgatgcct cttcttcctc
     1681 gtcgtcgatc accgcggctt ccgccttcgg cagatagagc gagaccgtcg tgccgcggcc
     1741 gagggcgctc tcgatggtcg ccgtcccgcc ggattgcttg acgaagccgt agatctgcgc
     1801 gaggccgagg ccggtgcctt tgccgatgtc cttggtggtg aagaacggct cgaaggcgcg
     1861 gcgccgcacc tcgggcgcca tgccggcgcc ggtatcggtg acgtcgacga cgatgtaagg
     1921 accgggcgcc acatcgagcc gccgcgcctc gatctcgcca gccgtctcgt tccgcgcccg
     1981 gatggtgagg cgtccgccct tcggcatcgc gtcgcgcgcg ttgatcgtca ggttgaggat
     2041 cgccgcctcg aattgcgcgg gatcgagatg cgccggccag agcccgggcg cggcggagac
     2101 ctcgagcgcg atcgcctcgc tgatgacgcg gcgcaccagc tcgccgaccg cgacgatcac
     2161 gcgatccatg gcgatggcgc gcggctgcag ctgctggcgc cgcgagaagg cgaggagctg
     2221 gctcgtgagc tgcgctcccc gctcggccgc gcgctgcgcc gacgccgcca tgcgcaggat
     2281 gcgcggatct tccttcgcga ccagcatgat cgactcgatc tggcccagca cgaccgtgag
     2341 cagattgttg aagtcatggg cgacgccgcc cgtgagctgg cccagcgact cgaggcgctg
     2401 cgcctgctgc agcgccgcct cgaccttctg gcgctcggcc acttccgctt cgagcgcgcg
     2461 cgagcgttcg tcgatctttc gctcgagcgt gtcgctcaga tcgcgatagg ctgcttccgt
     2521 ctctttctgc tcgtggatgt ccgtgctcga gccgatccac atcgcaatcg ccccgtcctc
     2581 ctcgcgcacc gggtgaacgc gggtcagatg ccagcgatag gcgccgtcgc ggcggcggaa
     2641 gcgatgcgcg tattgaaacg gctcgccagt cgcgagggag tgccgccagc gcacgacgtt
     2701 ctcggcgttg tcgtcgggat ggatgatccg cgcccaaccc aatcccatga tctcttcgag
     2761 cggcaggccg gcgaattcca gccattggcg attgcaataa tccacgaggc cgtccgcggc
     2821 tgtcgtgaat atcttctgcg gcatcgcttc cgcgaggaag cggaagcgat gctccgacat
     2881 cgccgccgct tcgcgccgca cccgcgccat ctcgagattg gcggcgacgc gcgccatgag
     2941 ctcgcgggcg ctgaacggct tgatgaggta atcgtcggca ccggcctcca ggccctcgat
     3001 gctggcctcc tcgcccgccc gcgccgagag caggatcacg ggaagccgcg ccagcctggc
     3061 atcgctgcgc accgcgcgga gcagctcgaa gccgctcatg cgcggcatca tgatgtcgct
     3121 cagcagaaga tcgggcagcc gcgcttcgag cgccgccagc gcctgcgcgc cgtcggcgac
     3181 cgcgcgcacg tcatagcgcg cgctcaggag gtggcggaca tagtcgcgca tatcggcgtt
     3241 gtcgtcggcg agaagaatgg tcgcgcgccc gcttttctga tcgaccgacg ccgccggcag
     3301 cgcctccgac aggagcggcc gctcgatgcc gggctcttcg ctgcgccctt catccggcag
     3361 ccagcgcaag gcttccttga catagggttg cgcgccgagc gcggtcgagg cgagcgtccg
     3421 ctccgcgcgc agccgctccg gcggcagatg cgccgtgccg agcgggatgc tcacaatgaa
     3481 gcggctgcct ttgccatggg tgctctcggc gcgcaccgcg ccgccatgga ggcgcaccag
     3541 ttcctgcacc aaagcgaggc cgatgccggt gccttcttgc gtgcgccccc gcgcgccctc
     3601 gacccgatag aagcgatcga aaagccgtgg gatctccgtc tcgggaatgc cggtgccggt
     3661 gtcctcgacc tcgagcaccg cgctggcacc cgcgcggcgg agccgcacgg cgatgccgcc
     3721 ctcgagggtg aacttgaacg cattcgagat gaggttgagg acgatcttct cccacatgtc
     3781 gcgatcgacc caggccggct cgccgagcgg cgggcagtcg accgtgaggc tcaacccggc
     3841 tttgtcggtc gccgcgcgga agacgctggc gagatcggcg gtggccgccg caagatcgag
     3901 cggctcgaag gacgcctgca cgcggcccgc ctcgatgcgc gagaaatcga ggagcgtgtt
     3961 gaccagccgc agcagcctca ggctgttgcg gtgaacgaga gcgaggtcgt cgcggtgcac
     4021 cggcaagctc tcggccggcg ccgccaacgc gtcctcgagc ggccccagca tcagcgtcag
     4081 cggcgtgcgg aactcgtggc tcacattgct gaagaaggcc gtcttggcgc ggtcgatctc
     4141 ggccagcgcc tcggcgcgct tgcgctcttc ctcataggcg cgggcattgg cgatggccgt
     4201 cgcgacctgc gccgccacca gatcgaggaa gctgcggtag agctcgtcga ggcgcaggcg
     4261 cggactcacg cccgcgacca gcagcgccgc cagctcatgc accttgttcg agcgcaccgg
     4321 cacgacgacc gcggcatgcg gcggatcgga ccagggtccc ggcggcacgg cgccaaagcg
     4381 tgctgccagg tcctcgacgc tcaccatctc ttcgcggcgc atcgcctcgg ccaagggcca
     4441 gccgcaggcg ggatcggccg cgtcgtcgag ctcgacgacc agcgggcttg ccgcctcgcc
     4501 gcggtcgatc ccggccgacc aggcgagccg cgcgcgcttg ccgtcgcgat cgatcagata
     4561 gagcagggcg aagggaatgt ccttcgcgtg attggccagc gtccggccgg cgatgacgca
     4621 ggcctgctcc gccgtcttgg cctccgccgc ccgcgccccc aggtcgcgga gcgcgacgat
     4681 gcgccgctcg ccgaccacct tctcggtaat ctcgtgcacc gtcgcgagca cgccgccgat
     4741 gccgcgcggc gccgcctcgt cggggacggg gctgtaggcg atggtgaaat gcgtctcctc
     4801 gagaaagcca tgccggttga cctcgaggag gatgtcctcc atccaggtcg cgggaccgcc
     4861 gtcgaacggc gtgtcgatca gcggacgcag gatgtgccag atctccggcc agcaatcgca
     4921 ggcgcgctga cccatggatt tcgggtgctt ggtcccggga acgggggcat aggcatcgtt
     4981 gtagagctgg acgtagtccg gtccccacca cagcagcatg gggaagcggt tggcgagcat
     5041 catgctgacg atggcgcgga gcgcctggga ccagctctcg accgggccca gcggcgtcgt
     5101 cgaccagtcg atcgcgcgca tgagcgcgcc catctcgccg ccgccggcca ggcaggtgtg
     5161 cgcgccgcgc ttccctttcg tcattgccgt ggtcagacgt ctccccctac cgcccgcgat
     5221 tattggcctt cggcggagac cggcaagaac ccactaagta cgagcagcga ccgcacaaat
     5281 ggcgcgcggc ggtagtcttt cgcgctacaa gtgctactgg aggatggccg gcgcctttgc
     5341 cagacggtcg aaggcgatga gctgctcgag cagcgccggc aggtcctgga gccgcaccat
     5401 gttgggcccg tcggacggcg cgtggtcggg atcctggtgc gtctccatga agacggcggc
     5461 gacgccgatc gcgcaagcgg cgcgcgccag gatgggaacg aagcggcgct cgccgccggt
     5521 cgaatccccc ttgccccccg gctgctgcac ggaatgggtg gcgtcgaaca ccacgggata
     5581 gccggtctcg gccatgatcg gcagcgcgcg catgtcgttg acgagcgtgt tgtagccgaa
     5641 agagacgccg cgctcgcaca acaggatgtt ctcgttgccg gccacggcga tcttgtgcgc
     5701 gacgttcttc atgtcccagg gcgccaggaa ctggcctttc ttgacgttga tggcgcggcc
     5761 ggtcttggcc gcggccacca ggagatcggt ctggcggcag agaaaggccg ggatctgcag
     5821 gacgtcgacc gcttcggcga cgcgcgcgca ttgctcgggc gaatgaacgt cggtcagcac
     5881 cgggcaacca taggtttcgc gcacttcggc gagaatcggc aggctcgcct cgaggcccaa
     5941 gccccgctcc gccgcgatcg aggtgcggtt cgccttgtcg aaggaggtct tgtagatgag
     6001 gccgagcccg agcttgccgg tgagctcggt cagcgcatgg ctcatctcga gcgcatgctg
     6061 ccggctctcg agcacgcaag gcccggcaat gagcgccacc ggccggtcat tccccagcgt
     6121 cagattgccg atatggacat gtctatgatg catgggcgcc tttctagcac ttcagctagg
     6181 taatgtggag attcgccacg cgacctcgtc gaggagcgaa gccctcatta cgagcgaacg
     6241 cagagggcgc cgaggatacg cggaggtcgc agaggatgac gattcctccg cgccctctgc
     6301 gtatcctcgg cgtcctctgc gttcctcttt tagatctctg ccattcccgt cacaccaagc
     6361 ggctctggtc gcgcgccgcg cggatgaagg acgtgaagag cggatgcggc tcgaagggct
     6421 tcgatttgag ctcggggtga tattgcacgc cgatgaacca gggatggccc ggcagctcga
     6481 cggtctcggg cagctcgcca tcgggcgaca tgccggagaa gagcaggccc gcccgctcga
     6541 gccgcgcctt gtaattgacg ttgacctcgt agcggtggcg atggcgctcg ctgacgcgct
     6601 gcgcgccgcc ataggcttcg ctgacctggc tgcccggcgc cagcatcgcc tcataggcgc
     6661 caaggcgcat gctgccgccg agatcgccgc cggcgcggcg ccgctccagc gtattgccgc
     6721 gcatccactc cgtcatcagg ccgacgaccg gatgctcgca aggaccgaac tcggtcgagc
     6781 ccgcgccggg caagcccgcc agatgccgcg ccgcctcgat caccgccatc tgcatgccga
     6841 agcagatgcc gaaatagggt acgtgcttct cgcgggcgaa gcgcgccgct tcgatcttgc
     6901 cttcgctgcc gcgctcgccg aagccgccgg gcacgaggat gccatgcacg ccttcgagct
     6961 gctgcacggc gtccggctgc tcgaagatct cggaatcgag ccagttgagg ttgacgcgca
     7021 cctggttggc gatgccgcca tgggtgagcg cctcggcgag cgacttgtag ctgtcgagga
     7081 gatgcgtgta tttgccgacg acggcgatgt tgaccacgcc ctcgggcttg cggatgcgcg
     7141 tcacgatctc gcgccagcgc ccgagatcgg gctcggccat gtcgatgccg aaatgcgcgc
     7201 agacctcgcg gtcgaagccc tcggcgtgat aggcgttggg cacttcgtag atggtgtcga
     7261 cgtcgagcgc cggaatgacg cgcgccgggt tgacgttgca gaagagcgcg atcttgcgcc
     7321 gctggctctc cgggaccggc cggtcgcagc ggcacagcag gatgtcgggc tggatgccga
     7381 gcgccagcag ctccttcacc gaatgctgcg tcggcttggt cttgagctcg ccggccgagg
     7441 gtatccacgg caccagagtc aaatgcacat agagcgtgcg ctcgcggccc aaatcattgc
     7501 cgagctggcg gatcgcctcg aggaagggca ggctctcgat gtcgccgacc gtgccgccga
     7561 tctcgcacag cacgaaatcc tcgtcgccat cgagatcggc ctggatgaat tccttgatgg
     7621 catcggtgac gtgcgggatc acctgcaccg tggcgcccag atactcgccc cgccgctcgc
     7681 ggccgatcac cgtcgagtag atgcgcccgg tggtgacgct gtcgctgcgc cgcgccgcga
     7741 ccccggtgta gcgctcgtag tggccgagat caagatcggt ctcggcgccg tcatcggtga
     7801 catagacctc gccatgctgg taagggctca tggtgccggg atcgacattg agataggggt
     7861 cgagcttccg cagacgcacc ttgaagcggc gcgcctgaag cagcgcgccg agtgacgctg
     7921 ccgagagacc tttacccagg gaggagacca cgccgccggt aatgaaaatg aaccgcgcca
     7981 tggaatttca gtctacagga tccgctttgc gcggcacaac ggaaagcgct gcacgccagc
     8041 gcgggcggcg gaaatcaacg ggcgaaagcg cggcgcgcgc gacgtcccac ggccggaggt
     8101 ggcgttctct cctgcgactc gctacttcgc cgctggggcg ccgggctcgt tggtcggcgc
     8161 cggcgccgca ggctcgggcg tgctcggtgc cgccggctcc gtcgcggccg gcggctgatc
     8221 gatcaccgag cgcggcgcgc tcgaggcgcc gcccgccaga taggcgaggg cgatgctcgt
     8281 cacgaagaaa gcgccggcga gaatcgccgt ggtccgcgtc aggagattgg ccgtggagcg
     8341 tccggtcatg aagcccgaca tgccgccgcc ccccatcccg agtgcacccc cctcgctctt
     8401 ctgcagcagg atgaccgcga cgagcgagac cgcgaccatg agatgaacga cgagaatgac
     8461 ggtggtcatg gcccgcgttt tagcgaatcc ggccgcgcta tgccagagcg gagctcaacc
     8521 ctcctctcct cagcgtcctc tgcgtaacct cggcgtcctc cgcgttcctc tttctttgcc
     8581 gcccccctac tccaccgcct tggcgatcgc ccagaaatcc tccgcgacga ggctcgcccc
     8641 gccgaccagc gcgccgtcga cctcgggcgc cgtgaggagc gcccgcgcat tcgccggctt
     8701 gaccgagccg ccatagagga tgcgcacggc cgcggcatcg gcgacgcgtt ttccgagcgc
     8761 ggcgcggata tgcgcatgca ccccggcgat gtcggccggc gtcggcgtgc gtccggtgcc
     8821 gatcgcccag atcggctcat aggcgatcac cagctctgcc gcgccgagcc cctgcggcaa
     8881 agagccgccg agctgccgcg cgaccaccgc gagcgtctgg cccgcatcgc gttcttccgc
     8941 cgtctcgccg atgcagagga
//
LOCUS       DASYNS010000526         8076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4654494, whole genome shotgun sequence.
ACCESSION   DASYNS010000526 DASYNS010000000
VERSION     DASYNS010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4654494"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VGP50_16235"
     CDS             <1..258
                     /locus_tag="VGP50_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019460633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEV8028983.1"
                     /translation="ASHAWLAVWCGGEQWLDLDPTNNRPTSTDHVTVAWGRDFEDVSP
                     VRGVLFGGGRHSLAVMVDVDPVRGESPAPGDAQADVPSSRV"
     gene            complement(228..719)
                     /locus_tag="VGP50_16240"
     CDS             complement(228..719)
                     /locus_tag="VGP50_16240"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8028984.1"
                     /translation="MQQPGDTLRRFVVPPASDGRIRRLFEYWRSIQPAPDRLPGRQHL
                     EPLDVPELLQWLSLVDVVGAPPRFRYRLVGTAQARAMQCDLTGRWVDEAYDGFRDRRD
                     HAEFVAVAGGEIRYSRGAPEHRVERSHVLMERLLLPLARDGASVDMILGMALYTRDDG
                     TSA"
     gene            930..1121
                     /locus_tag="VGP50_16245"
     CDS             930..1121
                     /locus_tag="VGP50_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HEV8028985.1"
                     /translation="MEYAFPVLVLVAMFAVLGVLLLGVINMARGNNPRRSQTLMRSRV
                     VLQGLALLLFALFMFLYRR"
     gene            1128..1691
                     /locus_tag="VGP50_16250"
     CDS             1128..1691
                     /locus_tag="VGP50_16250"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEV8028986.1"
                     /translation="MVRLTRIYTRGGDQGETSLGDGGRVPKQALRVDAFGTVDEANAA
                     LGLARLHATSESDVDAALARIQNDLFDLGADLATPEGGRANALRIVGAQVERLEREID
                     AWNASLSPLDSFVLPGGTPLAAHLHLARTVVRRAERLVAALAAAEPVNAEALKYLNRL
                     SDHLFVLSRHVNDRGARDVLWRPGAHR"
     gene            1803..2552
                     /locus_tag="VGP50_16255"
     CDS             1803..2552
                     /locus_tag="VGP50_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEV8028987.1"
                     /translation="MKILVAVKRVVDFNVKVRVKADRTGVETANVKMSMNPFDEIGVE
                     EAVRLKEAGVATEIVAVSMGPQGCQETIRTALAMGADRGIHVLSDAELQPLAVAKLLK
                     AIAAKEQPQLVILGKQAIDDDSSQTGQMLAALLGWAQATFASKLKVEGGKAQVTREID
                     GGLETIEIKLPAVLTTDLRLNEPRYASLPNIMKAKKKPIEQTTPEALSVDVTPRLVTL
                     KVEEPPKRQGGKKVGSVQELVDKLKNEARVI"
     gene            2552..3499
                     /locus_tag="VGP50_16260"
     CDS             2552..3499
                     /locus_tag="VGP50_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV8028988.1"
                     /translation="MALLVVAEHDNKTLKSSTLNAVAAATAIGGEIHVLVAGSNARPA
                     AEAAAKASGVAKVLLAEDPAYEHLVAEDLANLVVKLAPGYSHLLTAASSFGKNFMPRV
                     AALLDVAQISDIAAVQSPDTFVRPIYAGNALLTVQSKDRIKVITVRGTAFPPAAATGS
                     NAPIETVSAAGSAGLSRFIGAELSKSERPELTAARVIVSGGRGMQSGDNFKLLESLAD
                     KLGAAVGASRAAVDAGFVPNDYQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKVIV
                     AINKDEEAPIFQVADYGIVGDLFKIVPELDAELAKRQAS"
     gene            3546..4424
                     /locus_tag="VGP50_16265"
     CDS             3546..4424
                     /locus_tag="VGP50_16265"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEV8028989.1"
                     /translation="METIKNIGVIGAGQMGSGIAHVCALAGYTVKLADIDPAALESAV
                     QVIDRNLGRQVGRSVISDADKNAAMQRIETGTGYEMFGDCDIVIEAATEKEDVKRDIF
                     KKLVPQLKSDAIIASNTSSISITRLAAATDRPDRFIGMHFMNPVPVMALVELIRGIAT
                     DEKTFATVRNLALRMGKKPVAAEDFPAFIVNRILLPMINEAVYTLYEGVGSVDAIDTA
                     MKLGANHPMGPLELADFIGLDTCLSVMQVLYEGLADSKYRPCPLLVKYVEAGWLGRKA
                     GRGFYDYRGEKPVPTR"
     gene            4592..5155
                     /locus_tag="VGP50_16270"
     CDS             4592..5155
                     /locus_tag="VGP50_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008850808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV8028990.1"
                     /translation="MSLALDSCPALVLNADFRPLSYFPLSLWSWQETVKAVCLDRVNI
                     ISLYDRVVRSPSFEMRLPSVISLKEYIPTARRPAFTRFNVFLRDRFTCQYCGDDFSSH
                     DLTFDHVVPRSRGGRTTWENVVTACSTCNLLKGSRLPVESGMYPRHLPGQPTTHLLQE
                     NGRAFPPNFLHQSWRDFLYWDSELEAT"
     gene            complement(5175..5402)
                     /locus_tag="VGP50_16275"
     CDS             complement(5175..5402)
                     /locus_tag="VGP50_16275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028991.1"
                     /translation="MRMPGSLLPLAAAALIYGCAGGARDVDYGQERGISADIDATYQT
                     GPLLLKHGQIEHDEPYGAFPWQLDERHQDAE"
     gene            5519..6370
                     /locus_tag="VGP50_16280"
     CDS             5519..6370
                     /locus_tag="VGP50_16280"
                     /inference="COORDINATES: protein motif:HMM:NF027246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4743 domain-containing protein"
                     /protein_id="HEV8028992.1"
                     /translation="MSLRRHIRLCNDYRPERFMPLWRGATRIGLVRRDNAEALRRFPD
                     VFAVAPDGVRLVAAGDCAALTAAIDRVVERLLDVGRIAAWRNESFAVGACWGAPPIFK
                     LDRGAVPFFGVRAYGVHLNGYRHQGDVLELWIGRRAPDKKVAPDKLDNLVAGGIGNGH
                     GLFETLVKEAQEEASLPADLIAEAVAAGALSYRMETSQGVRDDVLFLYDLEVPLAFTP
                     RNTDGEIVAFEPIPATAVVERVRRSDDFKFNVNLVIIDFALRHGLVTPDDPEYLDLVL
                     GLHRPLD"
     gene            complement(6374..7237)
                     /gene="gluQRS"
                     /locus_tag="VGP50_16285"
     CDS             complement(6374..7237)
                     /gene="gluQRS"
                     /locus_tag="VGP50_16285"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEV8028993.1"
                     /translation="MNGVVTRFAPSPTGDLHLGHAYSALVGWQAARSVGGRFLLRIEN
                     IDRTRCRPEFTAGILRDLAWLGLDWDGAVRVQSEHFDDYRGALDRLASAGLLYPCFCT
                     RAAIKAEVTQAAAAPHGVEGPLYPGTCRSLAPAEREQRMAAGEAYALRLDAARATARS
                     GPLWWEDEIAGRIAADPSNHGDVVLARKDTPASYHLAVTVDDALQGVTLVTRGEDLFA
                     ATHVHRLLQALLDLPVPRYRHHRLRTDESGRRLAKRDRAATLKALREAGRTPAEVRAM
                     GGFPDGVAVRG"
     gene            7330..7995
                     /locus_tag="VGP50_16290"
     CDS             7330..7995
                     /locus_tag="VGP50_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV8028994.1"
                     /translation="MSLLRSLSGPSHKPAGGSKPRQLVVLLHGLGADGNDLIGLAPYW
                     TPLLPEAEFISPDAPFPCDMAPYGRQWFSFQNRSPAAILAGVRAAATILDAFLDEALA
                     ARNLDERSLALVGFSQGTMMSLYVGLRRQAALAGIIGFSGALVGADALPSEIRSRPPV
                     LLVHGDADEVVPFGALEASVRGLQAAGVPVEDVVRPGLGHGIDEEGLRRAAEFLRRIF
                     ARP"
BASE COUNT         1313 a         2795 c         2616 g         1352 t
ORIGIN      
        1 gcctcccatg cctggctcgc cgtctggtgc ggcggcgagc aatggctcga tctcgacccg
       61 accaacaatc ggccgacctc gaccgaccat gtgacggtgg cgtggggacg cgacttcgag
      121 gatgtgagcc cggtgcgcgg cgtgctcttc ggcggcggcc ggcacagcct cgccgtcatg
      181 gtcgatgtcg atcccgtgcg cggcgagtcg ccggcgcccg gcgacgctca ggcggacgtg
      241 ccgtcgtcgc gggtatagag cgccatgccc aggatcatgt cgacgctggc gccgtcccgc
      301 gcgagcggca gcagcagccg ctccatcagc acatggctgc gctcgacgcg atgctcgggg
      361 gcgccgcggc tgtagcggat ctcgccgccg gcgacggcga cgaactcggc atggtcgcgc
      421 cggtcgcgga agccgtcata ggcctcgtcg acccagcgcc ccgtcaggtc gcattgcatc
      481 gcccgcgcct gcgcggtgcc gaccaggcga tagcgaaagc gcggcggcgc gcccaccacg
      541 tcgacgagcg acagccattg cagaagctcc ggaacgtcga gcggctcgag gtgctggcgg
      601 cccggcaggc ggtccggggc cggctgaatc gagcgccagt actcgaagag ccgccggatc
      661 cggccatcag aggccggcgg cacgacgaag cggcgcagcg tatcgccggg ctgttgcacg
      721 ggccgaacct cctttgcggc agcaagacgc gccgggcgga tcaaacaacc agcgtagccg
      781 aaccaccgca ttcagccaga cgagatatct acaatttagc gaatttcccc gtcaaccatt
      841 cttctcaggg tcgcaatgcg cgggcatgtc gcctggggcg caattcggcc gcgctggcca
      901 gcggaaagcg gagcgctata ttgtcgcgca tggagtacgc ctttcccgtt ctcgttctcg
      961 tcgccatgtt cgccgtcctc ggcgtcttgc tgctcggcgt catcaacatg gcgcgcggca
     1021 acaatccccg gcgctcgcag actctcatgc gatcgcgtgt cgtgctgcag ggcctggcgc
     1081 tgctgctctt cgccctcttt atgtttctct atcgccgcta gacgctcatg gtgcgattga
     1141 cgcgcatcta cacgcgaggc ggcgaccagg gcgagacgtc gctgggcgat ggcggccgcg
     1201 tgccgaaaca ggcgctccgc gtcgacgcct tcggcacggt cgacgaggcc aatgccgcgc
     1261 tcgggctcgc ccgtcttcat gcgacgagtg aaagcgacgt cgatgccgcg ctggcgcgca
     1321 tccagaacga cctcttcgat ctcggcgccg atctcgcgac ccccgaaggc ggccgcgcga
     1381 acgcgctgcg catcgtcggg gcgcaggtcg agcggctcga gcgcgagatc gatgcctgga
     1441 acgcgtcgct cagccccctc gactccttcg tgctccccgg cggcaccccc ctggccgccc
     1501 atctccacct ggcgcgcacc gtggtgcgcc gcgccgagcg cctcgtcgcc gccctcgcgg
     1561 ccgccgagcc ggtcaacgcg gaagccctta agtatctgaa tcgcctgtcc gatcacctct
     1621 tcgtgctgag ccgccacgtc aacgaccgcg gcgcccgcga cgtcctctgg cgccccggcg
     1681 cccaccgctg actcaccccc ctcgatgatg cttgcgttac acgaaatgct tgccttcgcg
     1741 ttgtgcacgt gcacaattcc ccgccgcacc gcaccacgcc aaggctctga tcaaggagcg
     1801 ccatgaaaat tctcgtcgcg gtcaagcggg tggtcgattt caacgtgaag gtccgcgtca
     1861 aggccgaccg gaccggcgtc gagaccgcca acgtcaagat gtcgatgaat ccgttcgacg
     1921 aaatcggcgt cgaggaggcc gtgcgcctca aggaagccgg cgtggcgacg gagatcgtcg
     1981 cggtttcgat gggcccgcag ggttgccagg aaacaatccg caccgcgctc gccatgggcg
     2041 ccgatcgcgg catccatgtg ctgagcgacg ccgagctgca acccttggcc gtggcgaagc
     2101 tgctcaaggc catcgccgcc aaggagcagc cgcagctcgt gattctcggc aagcaggcga
     2161 tcgacgatga ttcgagccag acgggccaga tgctggcggc gctgctgggc tgggcgcagg
     2221 cgaccttcgc ctcgaagctc aaggtcgagg gcggcaaggc gcaggtgacg cgcgagatcg
     2281 atggcgggct cgagacgatc gagatcaagc tgccggcggt gctgaccacc gacctgcgct
     2341 tgaacgagcc gcgctacgcg tcgctgccca acatcatgaa ggcgaagaag aagccgatcg
     2401 agcagacgac gcccgaagcg ctgagcgtcg acgtcacgcc acgcctcgtc accctcaagg
     2461 tggaggagcc gccgaagcgc cagggcggca agaaagtcgg ctccgtgcag gagctcgtcg
     2521 acaagctcaa gaacgaagcg cgggtgatct gatggctctc ctcgtcgtcg ccgagcacga
     2581 caacaagacg ctcaagagct cgactctgaa tgccgtcgcc gccgccaccg cgatcggcgg
     2641 cgagatccat gtgctggtcg ccggatcgaa tgcgcggccc gccgccgaag ccgccgcgaa
     2701 agcgtccggc gtcgccaagg tgctgctcgc cgaggatccg gcctatgagc atctcgtcgc
     2761 cgaggatctt gccaatctcg tggtgaagct cgcgcccggc tacagtcacc tgctgacggc
     2821 cgccagctct ttcggcaaga acttcatgcc gcgcgtcgca gcactgctcg acgtcgcgca
     2881 gatctccgac atcgccgccg tgcagtcgcc cgacaccttc gttcgcccga tatacgcagg
     2941 gaatgcgctc ctcacggtgc agtcgaagga ccgcatcaag gtcatcaccg tgcgcggcac
     3001 cgcgtttccc ccggcagccg ccacgggcag caatgcaccg atcgagacgg tgagcgccgc
     3061 cggctcggcc ggtctgtcgc gtttcatcgg tgccgagctc tcgaaatcgg agcggccgga
     3121 gctgaccgct gcacgcgtca tcgtctcggg cggccgcggc atgcagtccg gtgacaactt
     3181 caagctgctg gaaagcctcg ccgacaagct cggcgcggcc gtcggcgcgt cgcgcgcggc
     3241 ggtcgatgcc ggcttcgtcc cgaacgacta ccaggtcggc cagaccggca agatcgtggc
     3301 acccgagctc tatatcgcgg tcggcatctc gggtgccatc cagcaccttg ccgggatgaa
     3361 ggattcgaaa gtgatcgtcg ccatcaacaa ggatgaagag gcgccgatct tccaggtcgc
     3421 cgactacggc atcgtcggcg acctgttcaa gatcgtgccc gagctcgatg cggagcttgc
     3481 caagcgccaa gcctcgtgac tttgcaccgg cgcttatgcg gagtgaggcc atgacctccg
     3541 atcgtatcga gaccatcaag aacatcggtg tcatcggcgc cggccagatg ggcagcggca
     3601 tcgcccatgt ctgcgcgctt gccggctaca ccgtgaagct cgccgacatc gaccccgcgg
     3661 cgctcgagag cgcggtgcag gtgatcgatc gcaatctggg ccgccaggtc ggccgaagcg
     3721 tcatcagcga tgccgacaag aacgcggcga tgcaacgcat cgagaccggc accggctatg
     3781 agatgttcgg cgactgcgat atcgtcatcg aggcagcgac cgagaaggaa gacgtcaagc
     3841 gcgacatctt caagaagctg gtgccgcagc tcaagagcga cgcgatcatc gcatccaaca
     3901 cctcctcgat atcgatcacg cgcctcgccg cggcaaccga ccggcccgat cgcttcatcg
     3961 gcatgcattt catgaacccg gtgccggtga tggcgctggt cgagctcatc cgcggcatcg
     4021 ccacggacga gaagactttc gccacggtcc gcaatctcgc attgcgcatg ggcaagaagc
     4081 cggtggccgc ggaggacttc cccgccttca tcgtcaaccg catcctgctg ccgatgatca
     4141 acgaggccgt ctacacgctc tatgagggcg tgggctcggt cgacgcgatc gacacggcga
     4201 tgaagctcgg cgccaatcat ccaatgggcc ccctggagct cgccgacttc atcggcctcg
     4261 acacctgtct ctcggtcatg caggtgctct atgagggtct cgccgattct aagtatcgtc
     4321 cctgtccttt gctcgtgaaa tacgtcgagg ccggctggct cggacgcaag gccggacgcg
     4381 gcttctacga ctatcgcggc gagaagccgg tgccgacgcg ttagggaccg cgccggtcgg
     4441 gttggcaata ttgtggcaat atgtttcggt ctattataat cgggctgatg gttgttgccg
     4501 gcttgccgtg tggcgcgtca atctggcgct gcttagccgt tcgtggcagg atgcggcgcg
     4561 cgatgaggtg cccgtaagat gaggtcgctg gttgtcgtta gcgctcgata gctgtcccgc
     4621 gctggtgctc aacgcagatt tccggccgtt gagctatttt cccttgtcgt tgtggtcctg
     4681 gcaggagacg gtcaaggcgg tgtgcctcga ccgcgtcaac atcatctcgc tctacgaccg
     4741 cgtcgtgcgc agcccctcct tcgagatgcg gctgcccagc gtcatctcac tgaaagagta
     4801 catcccgacg gcgcggcgcc ccgccttcac ccggttcaac gtcttcctgc gcgaccgctt
     4861 cacctgccag tattgcggcg atgacttctc ctcgcacgac ctgaccttcg atcacgtcgt
     4921 gccgcgctcg cgcggtgggc gcacgacatg ggagaacgtc gtgaccgcct gctcgacctg
     4981 caatctgctc aaaggcagcc gactgccggt ggagagcggc atgtatccgc gccacctgcc
     5041 gggtcagccg acgacccatc tgctgcaaga gaacgggcgc gccttcccac ccaacttcct
     5101 gcatcagagc tggcgcgact tcctctattg ggacagcgag ctcgaggcga cctgagcgcc
     5161 cgcacacgcc gccgctattc cgcgtcctga tgcctttcgt cgagctgcca cggaaaggcg
     5221 ccatagggct cgtcatgctc gatctggccg tgcttcaaga gcagcggccc ggtctgatag
     5281 gtcgcatcga tgtccgcgga gatgccgcgc tcctggccgt agtcgacgtc ccgcgctccg
     5341 ccggcacagc cgtagatgag ggccgcagcg gcgagcggga gcaagctgcc tggcattcgc
     5401 atggtgtcaa cctcctccct agggaacaat tgcccggccg tagcgctcca tgccgcggag
     5461 cgatggcggc tatccgcgct gttgacgcgt cgcttcggcc ggtgttgatc tctcgaaaat
     5521 gagcctccgc cgccacatcc gcctctgcaa cgactatcgc cccgagcgct tcatgccgct
     5581 ctggcgcggc gcgacgcgga tcgggctggt gcggcgcgac aacgccgagg cgctgcggcg
     5641 ctttccagac gtgttcgcgg tagcgcccga cggcgtgcgc ctcgtcgccg ccggcgactg
     5701 tgcggcgctc accgcagcga tcgaccgcgt cgtcgagcgg ctgctggacg tcgggcgcat
     5761 cgccgcgtgg cgcaacgaat ccttcgccgt aggcgcatgc tggggcgcgc cgcccatttt
     5821 caagctcgat cgcggcgccg taccgttctt cggtgtgcgc gcctatggcg tgcatctcaa
     5881 tggctaccgc caccagggcg acgtgctcga gctgtggatc gggcgccgcg cgccggacaa
     5941 gaaggtggcg ccggacaagc tggacaacct tgtcgccggt ggcatcggca acggccacgg
     6001 ccttttcgag acgctggtca aggaggcgca ggaggaagcg tcgcttcccg cggacctcat
     6061 tgccgaggcg gtggcggcag gcgccctctc ctatcgcatg gaaacgtcgc agggcgtgcg
     6121 cgatgacgtc ctcttcctct acgatctcga agtgccgctc gcctttacgc cgcgcaatac
     6181 cgatggcgag atcgtcgcct tcgagccgat acccgcgacc gcagtggttg agcgggtgcg
     6241 gcgcagcgat gatttcaagt tcaacgtcaa tctcgtgatc atcgatttcg cgctccgcca
     6301 cggcctcgtc accccggacg atcccgaata tctggatctc gtcctgggcc tgcaccggcc
     6361 gctcgactga ggctcatccc cggactgcga cgccgtccgg gaagccgccc atggcacgca
     6421 cttctgccgg tgtccggccg gcctcgcgca acgctttcag cgtcgctgcc cgatcgcgct
     6481 tcgcgaggcg gcggccgctc tcatcggtac gcaggcggtg atggcgatag cgtggcaccg
     6541 gcagatcgag cagcgcttgc agcaaccgat ggacatgggt tgccgcgaag aggtcctcgc
     6601 cgcgcgtcac cagggtcacg ccctggagcg cgtcgtcgac ggtgaccgcc aggtgatagc
     6661 tcgccggcgt gtctttccgc gcgagcacga catcgccgtg attcgacgga tcggcggcga
     6721 tccggccggc gatttcgtct tcccaccaca gcggaccgct ccgcgccgtg gcgcgtgccg
     6781 cgtcgagccg cagcgcatat gcctcgcccg ccgccatcct ttgctcgcgt tccgccggcg
     6841 caaggctccg gcaggtgccg ggatagagcg gcccctcgac gccatggggc gcggcggcgg
     6901 cctgcgtcac ctcggccttg atggccgccc gcgtgcagaa acacgggtaa aggagccccg
     6961 ccgacgcaag gcgatcgagg gccccgcggt agtcatcgaa atgctcggat tggacgcgca
     7021 ccgccccgtc ccagtcgaga ccgagccagg caaggtcgcg cagaatcccc gcggtgaatt
     7081 cagggcgaca gcgcgtgcgg tcgatgttct cgatgcgcaa caggaaacgc ccgccaacgc
     7141 tgcgcgccgc ctgccagccg acgagcgccg aataggcatg gcccagatgc agatcgcccg
     7201 tcggactcgg cgcaaagcgc gtgacgacac cgttcatgag gcgaacttag caagcgccgg
     7261 cacgtgacaa cgagcgacgc atctcctatg ccgttgtcgc agttggcaag attggcaaga
     7321 ggagcagcca tgtcgctttt gcgcagcctc agcggcccga gccacaagcc ggccggcggc
     7381 agcaagccac gccaactcgt cgtgctgctg catggcctgg gcgccgacgg caacgacctc
     7441 atcggcctcg ctccttactg gacccccctg ttgcccgagg cggagttcat ctcgcccgat
     7501 gcgccttttc cctgcgatat ggcgccctat ggccgccaat ggttcagctt ccagaaccgc
     7561 tcgcccgccg cgattctggc gggtgtccgc gccgccgcca cgatcctcga cgccttcctc
     7621 gacgaggcgc tcgccgcccg caacctcgac gagcgcagcc ttgcgctggt gggattttct
     7681 cagggtacga tgatgtcgct ctatgtcggc ctgcggcgtc aggctgcgct tgcggggatc
     7741 atcggcttct cgggagcgct ggtcggcgcc gacgcgctgc cgtcggagat ccgctcgcgc
     7801 ccgcccgtgc tgctcgtcca cggcgacgcc gatgaagtgg tgccgtttgg cgccctcgaa
     7861 gcctcggttc ggggcttgca ggccgcgggc gttcccgtcg aggatgtcgt ccgacccggt
     7921 ctcggccatg gcatcgacga agagggactg cgccgcgccg ccgagttcct gcgccgcatc
     7981 ttcgcgcgcc cctgaaccgc cggtgcccgg tcagcggact cccgatgtcg atgtcttcac
     8041 gacaccggac gcgctggcgc cctgcagata ccgctc
//
LOCUS       DASYNS010000527         5067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4503232, whole genome shotgun sequence.
ACCESSION   DASYNS010000527 DASYNS010000000
VERSION     DASYNS010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4503232"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VGP50_16295"
     CDS             complement(<1..220)
                     /locus_tag="VGP50_16295"
                     /inference="COORDINATES: protein motif:HMM:NF015836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028995.1"
                     /translation="MHLDAVARPPLERSIAPWCLAALCLLGFLLRLVPVLFYPSLNFP
                     DEIFQTAEPAHRLVFGSGLVPWEFAYGVR"
     gene            complement(314..595)
                     /locus_tag="VGP50_16300"
     CDS             complement(314..595)
                     /locus_tag="VGP50_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028996.1"
                     /translation="MKRITKVVVGQRFRSIGVTGKPTYAFEVQTTFRSSVDQREYVRL
                     VEVADPTRTKTLALATITDPRHFVAIQPEGENQRPPSRGFGRAILDALR"
     gene            complement(711..2447)
                     /gene="ilvD"
                     /locus_tag="VGP50_16305"
     CDS             complement(711..2447)
                     /gene="ilvD"
                     /locus_tag="VGP50_16305"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV8028997.1"
                     /translation="MPDEARRPSYDKSTLPSRHVTVGPERAPHRSYLHAMGLSDEQIA
                     QPLIGVVTTWNEAAPCNISLSRQAQAAKKGVAAAGGTPREFTTITVTDGIAMGHAGMK
                     SSLVSREVIADSVELTMRGHCYDAMVGLAGCDKSLPGLMMAMVRLNVPAVFMYGGSIL
                     PGRFRGRDVAVGDLFEAVGAHSAGLMSDEDLHEFECVACPSAGSCGGQYTANTMACVS
                     EAIGLALPGSAGAPAPYESRDRYAEASGEMVMRLLRHRLRPRDIVTPKALENAAMVVA
                     ASGGSTNGALHLPAIAHECGIDFDIHAVAAIFRRTPYIADLKPGGRYVAKDLYDIGGV
                     PVLMKALLDGGYLHGDCITVTGKTIAENLAEVRFPADQDVVRPTATPLSTWGGVVGLR
                     GNLAPQGAIVKVAGMTELKFRGPAHCFDCEEDAFAAVEARNYKAGEVIVIRYEGPRGG
                     PGMREMLSTTAAIYGQGMGDKVALITDGRFSGATRGFCVGHVGPEAAVGGPIGLLRDG
                     DIIAIDAAAGTLAVELSDAELAQRRSAWTPRSHDYQSGALWKYAQTVGDAEKGAVTHP
                     GAKAETHVYADI"
     gene            complement(2579..4294)
                     /locus_tag="VGP50_16310"
     CDS             complement(2579..4294)
                     /locus_tag="VGP50_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV8028998.1"
                     /translation="MSERAAAVKASVGFASEAGPRPRNEDFAGAVLGSELPEPRRDVV
                     SAIADGIGGAKGGRVAAEMAVRGFLDGFCDLPETLEVRRAAAKVLGALNGWIHGQGRH
                     DPALAGMGCTFTALVLRGRVAHVLHVGDTRAYRMRRDRLSCLTSDHVREGGAGGSGML
                     YRALGVEAELRLDYASHPVALHDRFLLCSDGLYGFLTDEAIADILRERSAPGDTARAL
                     VTAALQSGSSDNCTALVADLVALPARDSADIGAALMQLPVRPVPNSGETVDGFVLKTL
                     ISDGRYTRLFAALDEVEGGDVALKFPKPQVAAVETYHAAFVREAWVGTRVNSPWVGHV
                     IELPAGRQTSLYTVMPLYVGELLENRLARRPGLGLEEGRNIAIKLARAAGALHRAGVI
                     HRDIKPDNVILEDAGALKLIDLGVVRIPGLEDFPGGDIPGTPAYMAPEMFSGEAGNER
                     TDIYALGVTMFRAFTGEYPYGNLDAISPPRRSRAKELSVLRPDLPAWLQAAVGRAIAV
                     DPNERFPDMMEFAVEMESGPARSPPVGAQRPRTLYERYPLQFWQGIAALLGFALLLSL
                     WRHQG"
     gene            complement(4367..4690)
                     /locus_tag="VGP50_16315"
     CDS             complement(4367..4690)
                     /locus_tag="VGP50_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8028999.1"
                     /translation="MSILRPLLIATLAALIAAAATGAGAQPGEAAITCTNPSSGTTWQ
                     IRIDYQKATVDQYPASITDAEISWHDASDGGNYTLDRKSGNLRVAVASSTGGYFLHHR
                     CQLEN"
BASE COUNT          849 a         1685 c         1719 g          814 t
ORIGIN      
        1 agcggacgcc ataggcgaat tcccagggca cgaggccgct gccaaagacg agccgatggg
       61 ccggctccgc cgtctggaag atctcgtcgg ggaaattcag gctcggatag aagaggaccg
      121 ggaccagccg cagcaggaag ccgaggagac acagagccgc caggcaccac ggggcgatgc
      181 tgcgctcaag cggcggccgg gcgaccgcgt cgaggtgcaa aatggtctct cctgccgggg
      241 ccacgaggcc agacggccgc gcgcccaccg attaagcggc aggttgatta atccgcggtt
      301 tatgagttgc gggtcagcgc agggcgtcga ggatggcgcg gccgaacccc cgcgagggcg
      361 gccgctggtt ctcgccttcg ggctggatcg ccacgaaatg gcgcgggtcc gtgattgtgg
      421 caagagcaag cgtcttggtt cgggtcgggt cggccacctc gacgagacgc acgtattcgc
      481 gctggtcgac gctgctgcgg aaggtcgtct ggacctcgaa ggcgtaagtc ggcttgcccg
      541 tgacgccgat cgagcggaac cgctggccga cgacaacctt ggtaatgcgc ttcatcttgc
      601 tcctcgtatg cggccaagac ttcgaacgac accgctgacc gagcgagagc ctaggcccgg
      661 gatggtaaac aggatctaaa cgtgcctctg accctcgtcg cagcgctgtg tcaaatatcg
      721 gcgtagacgt gagtttcggc cttggcgccg ggatgggtga ccgcgccctt ctccgcatcg
      781 cccacggtct gcgcgtattt ccacaaggcg cccgactggt agtcatggct gcgcggcgtc
      841 cacgcgctgc gccgctgcgc cagctcggca tcgctcagct ccaccgcgag agtgccggcc
      901 gcggcgtcga tggcgataat gtccccgtcg cgcaacaggc cgatcggccc gcccaccgcc
      961 gcttcgggac cgacatggcc gacgcagaag ccgcgcgtcg cgccggagaa gcggccatcg
     1021 gtgatgagcg cgaccttgtc gcccatgcct tggccataga tcgccgcggt cgtcgacaac
     1081 atctcgcgca tgcccggccc gccgcgcggg ccctcatagc ggatgacgat cacttcgccc
     1141 gctttgtagt tgcgcgcctc gaccgccgcg aaggcgtcct cctcgcaatc gaagcaatgc
     1201 gcgggcccgc ggaacttcag ttcggtcatg ccggccactt tgacgatcgc accctgcggc
     1261 gcgaggttgc cgcgcaggcc gacgacgccg ccccaggtcg agagcggcgt cgcggtcgga
     1321 cgcaccacgt cttgatccgc ggggaagcgc acctcggcga gattctcggc aatggtcttg
     1381 ccggtgacgg tgatgcagtc gccatggaga tagccgccat cgagcagcgc cttcatcagc
     1441 accggcacgc cgccgatgtc gtagagatcc ttggccacat agcggccgcc cggtttcaaa
     1501 tcggcgatat aaggcgtgcg ccggaagatc gccgccacgg cgtggatgtc gaaatcgatg
     1561 ccgcattcat gggcgatcgc cggcagatgc agcgcgccgt tggtcgagcc gccggacgcc
     1621 gccaccacca tggccgcgtt ctcgagcgcc ttcggcgtca cgatgtcgcg cgggcgcagg
     1681 cgatggcgca gcagccgcat gaccatctcg cccgaggcct cggcatagcg gtcgcgcgac
     1741 tcgtaaggcg cgggcgcacc ggccgagccg ggcagcgcga ggccgatcgc ctccgacacg
     1801 caagccatgg tgttggcggt gtattggccg ccgcaagagc cggccgaggg acaggcgacg
     1861 cattcgaact cgtgcagatc ctcgtcgctc atgaggccgg cgctgtgcgc gcccaccgcc
     1921 tcgaagaggt cgcccacggc aacgtcgcgg ccgcggaagc gtccggggag gatcgagccg
     1981 ccatacatga agaccgcggg cacgttgagc cgcaccatcg ccatcatcag cccgggcagc
     2041 gatttgtcgc agccggcgag tccgaccatg gcgtcatagc aatggccgcg catggtgagc
     2101 tcgaccgaat cggcgatgac ctcgcgcgag acgagcgacg atttcatccc ggcatggccc
     2161 atggcgatgc cgtcggtgac ggtgatggtc gtgaactccc gcggcgtgcc gcccgccgca
     2221 gccacgcctt tcttcgcggc ctgcgcctgc cgcgacagcg agatgttgca gggcgccgcc
     2281 tcgttccagg tggtgacgac gccgatcagg ggctgcgcga tctgctcgtc gctcaggccc
     2341 atggcatgga gataagagcg gtgcggcgcc cgctccggcc ccacggtcac atggcggctg
     2401 ggcagcgtcg acttgtcgta actcggtctc ctcgcctcat cgggcatggc ggctctcctt
     2461 ttacttttac gcgggctcga ccgccacaca ccatacagag ccggcgcgcg cgttggctag
     2521 gctacgcgct ggcgacgcac atatgcctgt accgccagcg acgatgcttc gcatcgcgct
     2581 agccttgatg ccgccacagc gacagaagga gcgcgaagcc cagcagtgcc gcgatccctt
     2641 gccagaactg cagcggatag cgctcgtaga gcgtccgcgg ccgctgcgcg ccgaccggcg
     2701 gcgagcgcgc cggccctgat tccatctcga ccgcgaattc catcatgtcg gggaaacgct
     2761 cgttcggatc gacggcgatg gcgcgcccga ccgctgcctg cagccaggcc ggcaggtcgg
     2821 ggcggagaac ggagagctct ttcgcgcgcg accggcgcgg cgggctgatg gcatcgaggt
     2881 tgccgtaagg gtactcgccc gtgaaggcgc ggaacatggt gacgcccagc gcatagatgt
     2941 cggtgcgctc gttgccggcc tcgcccgaga acatctcggg cgccatgtag gcgggcgtcc
     3001 cgggaatgtc gccccccggg aaatcctcca atcccgggat gcgcacgacg cccagatcga
     3061 tgagcttcag cgcgcccgcg tcttcgagga tcacgttgtc gggcttgatg tcgcgatgaa
     3121 tgacgccggc acggtggagc gccccggcgg cccgggcgag cttgatggcg atgttccgtc
     3181 cctcttcgag gccgagcccc ggccggcgcg ccagccggtt ctccagaagc tcacccacat
     3241 aaagcggcat gaccgtatag agagaggtct gccgccccgc cggcagctcg atgacatggc
     3301 cgacccacgg gctgttgacg cgcgtgccca cccaggcttc gcgcacaaag gccgcatggt
     3361 aggtctcgac ggcggccacc tggggcttcg ggaacttcaa cgccacgtcg ccgccctcga
     3421 cttcgtcgag cgcggcgaag agccttgtgt atcgaccgtc cgaaatcagc gtcttcagca
     3481 cgaagccgtc gaccgtctcg ccgctgttcg gcaccggtct caccggcagc tgcatcagcg
     3541 cggcgccgat gtcggcggag tcgcgcgccg gcagcgcaac gaggtcggcg acgagggccg
     3601 tgcaattgtc gctgctgccc gattgaagcg ccgcggtcac caaggcccgg gcggtgtctc
     3661 ctggtgccga gcgctcgcgc aggatatcgg cgatggcctc gtcggtgagg aagccgtaga
     3721 gcccgtcgct gcacagcaga aagcggtcat gcagtgccac gggatggctc gcataatcga
     3781 gacgcagctc ggcctcgacg ccgagcgcgc ggtagagcat gcccgagccc ccggcgccgc
     3841 cctctcgcac atggtcgctt gtgaggcagc tcaagcggtc acgccgcatc cggtaggcgc
     3901 gggtatcgcc gacatgcaga acatgcgcga cgcgaccgcg cagcaccagc gcggtgaagg
     3961 tgcatcccat gccggcgagc gcgggatcat ggcgcccctg gccgtggatc cagccgttga
     4021 gcgcgcccag aaccttggcc gcggcgcgcc gcacttcgag ggtctcgggg agatcgcaga
     4081 atccgtcgag aaagccgcgc accgccatct cggcggcgac gcgaccgcct ttggcgccac
     4141 cgatcccgtc ggcgattgcc gacaccacgt cgcgtcgcgg ctccggcagc tcgctgccga
     4201 ggacggcgcc ggcgaaatcc tcgttgcgcg gacgcggtcc cgcctcggac gcgaagccga
     4261 cgctggcctt gacggcggcg gcacgctcac tcatcgccgt tgctccgacg ctgcaagctg
     4321 acttgccgtt gcggcgccgt gcagcggccg ttccggcgtc gcagcctcaa ttctccagct
     4381 ggcagcgatg atggaggaaa tagccgccgg tgctggacgc cacggcgacc ctaaggttcc
     4441 cggatttacg gtcaagcgtg tagttgccgc catcggaggc atcgtgccag gagatctcgg
     4501 cgtcggtgat gctggccggg tattgatcga cggtggcctt ctggtagtcg atccggatct
     4561 gccaagtcgt gccgctcgac ggattggtgc aggtgatcgc cgcttcgcca ggttgtgcgc
     4621 ccgcccctgt cgccgcagca gcgatgagag cggcgagcgt ggcgatgagc aatggacgca
     4681 atatcgacat cgagacagtc tgcaaagaaa tggcctcgca aggcaagtct gccgatattt
     4741 taggcattgt gccggcttag aaggcagaat gccggcgtcc gattggggct gatcactgaa
     4801 ttagcatagg cggcatagtt cttgctggtt gatggcgatt tcggggaggg ttcctcgctc
     4861 gcctgcacca aagggggaaa gaatggcact ggtcccatca ccacaatggc tcgataccgg
     4921 agataacgct tggcagttgg cagccgcgac gttcgtcggc ttgcaaagca ttcccggact
     4981 tacggttctc tatggcggca tcgtcaagaa gaaatgggcg atcaactccg ctttcatggc
     5041 gatgtacgcc ttcgcgtcgg tgctcgt
//
LOCUS       DASYNS010000528         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_131872, whole genome shotgun sequence.
ACCESSION   DASYNS010000528 DASYNS010000000
VERSION     DASYNS010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131872"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="VGP50_16320"
     CDS             <1..1389
                     /locus_tag="VGP50_16320"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEV8029000.1"
                     /translation="RAAAMAPSARGAERYRRATLTFLTALAVKGVALLTLFFSVKLGL
                     SYLGSERFGLWVTALSAAALLSFATLGLDKGLLNALAAADGSRNRAAARRLVSTTFFL
                     FLAIVAALLLLLAPAYPLVPWARLLNAGAGAAEAAPMMAVLIVCTLLALLGSLVDTVQ
                     SAYQEGFINGAWEAVGKLLGLGALAAAIAFGAGLPTLALAFAGAPLLAALGNALLLFG
                     RRRPWLAPRLRLVQRSVARRLLGAGTLFFLSQLALSVAYYVDNLIVASLAGSEAVAAY
                     AVTARLFDFPGMLLLLIGGALWPPLAEALAQGDISWAERGLGRLILASLALAGATALP
                     LILLGPQVIRWWVGGEIAPPGSLFAAFGVFWMLSALTQPIGVFLSAANALRFQLACVT
                     LLAAGGLVLKLLLAQRFGMEGVAWGRVVAEALFLLLPYALLLPRVLHELRGRAAMLSV
                     RPSTLRQAQDSG"
     gene            complement(1498..2214)
                     /locus_tag="VGP50_16325"
     CDS             complement(1498..2214)
                     /locus_tag="VGP50_16325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV8029001.1"
                     /translation="MKTSDIECEADGARLVGYLAVDVATVGKRPGVLVAHEGFGLNDH
                     AKAIARRLAEAGYIAFALDYHGDGKPLTDIALAMSRIRGWIADPTGSLARGRAGLEIL
                     LRQKETDASRVAGIGYCFGGTLVLDMARSGEDLKVVVGFHSGLGTGRAAAPGGVKSKV
                     LVQIGAEDPVIPAEQRLAFEKEMTAAGVDWRMILYSGAGHSFTNPSANLFGRPGFGYH
                     EPSDRRSWRAMLDALDEVFA"
BASE COUNT          273 a          802 c          790 g          426 t
ORIGIN      
        1 cgcgcggccg cgatggcgcc gagcgcacgc ggcgccgagc gctatcgccg cgccaccctc
       61 accttcctga cggcgcttgc cgtcaaaggc gtggcgctcc tgaccttgtt cttcagcgtc
      121 aagctcggcc tctcctatct cggcagcgag cgcttcggcc tgtgggtgac ggcgctctcg
      181 gcggcggcgt tgttgagctt cgccacgctc gggctcgaca agggcttgct caacgcgctc
      241 gccgcggccg acggaagccg caaccgcgcc gccgcgcgcc gtctcgtgtc gacgacgttc
      301 ttcttgtttc tggctatcgt cgcggcgctt ctgctgctgc tggctccggc ctatccgctc
      361 gtgccctggg cgcgtctcct caacgcgggc gcgggcgcgg cagaggccgc accgatgatg
      421 gcggtgctga tcgtctgcac gctcctggcc ttgctcggca gcctcgtcga caccgtgcaa
      481 tccgcttacc aggaaggctt catcaacggc gcgtgggagg cggtggggaa gctcctcggg
      541 ctcggtgcgc tggcggcagc cattgcgttc ggcgccggcc ttcccacgct cgctctggcc
      601 ttcgccggag cgccgctcct ggccgcgctc ggcaacgcgc tgctgctctt cggccgccgc
      661 cggccctggc tcgcgccacg ccttcggctc gtgcagcggt cggtcgcgcg gcggctcctg
      721 ggcgccggca ccctgttctt cctgagccag ctcgcgctca gcgttgccta ttacgtcgac
      781 aacctcatcg tcgcctcgct cgccggcagc gaagcggtcg cggcctatgc cgtgacggcg
      841 cggctcttcg attttcccgg catgctgctg ctgctgatcg gcggcgcgct ctggccgccg
      901 ctcgccgagg ccttggcgca aggcgatata agctgggccg agcgcggcct cggccggctc
      961 attctggcgt cgctggcgct ggcgggcgcg acggcgctgc cgctcatcct tctcgggccg
     1021 caggtgatcc gctggtgggt gggcggcgag atcgcgccgc cggggtcgct ctttgcggcg
     1081 ttcggcgtct tctggatgct gagcgcgctg acacagccga tcggcgtctt cctcagcgcc
     1141 gccaacgcgc tccgcttcca gcttgcctgc gtgacgctgc tcgccgccgg cgggctggtg
     1201 ttgaagctgc tgctcgcgca gcgcttcggg atggaaggcg tggcgtgggg ccgcgtcgtc
     1261 gccgaggcgc tgttcctgct gctgccttac gcgctgctgc tgccgcgggt gctgcacgag
     1321 ttgcggggcc gtgctgcaat gctgtccgtg cgtccttcga cccttcgaca ggctcaggac
     1381 tcaggatgag gattgttctt tatggcgtcg acaatatcga tgccatcctc cctcttcctc
     1441 attctgagtc ctgagcctgt cgaaggatcg aaggacgcac gccggtcatg cagcgcgtca
     1501 ggcgaagacc tcgtcgagcg cgtcgagcat ggctcgccag ctgcggcggt cgctcggctc
     1561 gtgataaccg aagccgggcc tgccgaatag gttggcgctg gggttggtga aggagtggcc
     1621 ggccccgcta tagaggatca tgcgccagtc gacgcccgcg gcggtcatct ccttctcgaa
     1681 ggcgaggcgc tgctccgccg ggatcaccgg atcctcggcg ccgatctgca ccagcacctt
     1741 gctcttgacg ccgccggggg ccgcggcccg tccggtcccg agaccggaat ggaagccgac
     1801 gacgactttg aggtcctcgc cgctgcgcgc catgtcgagc accagcgtgc cgccgaagca
     1861 atagccgatg ccggcgacgc ggctggcgtc ggtctctttc tggcgcagca ggatctcgag
     1921 gccggcgcgg ccgcgggcga ggctgcccgt cgggtcggcg atccagccgc ggatgcggct
     1981 catggcgagc gcgatgtcgg tgagcggctt gccgtcgccg tgatagtcga gcgcgaaggc
     2041 gatgtagcct gcctcggcca gacgccgcgc gatcgccttc gcatggtcgt tgaggccgaa
     2101 gccttcatgg gcgacgagca cgcccggccg cttgccgacc gtcgccacgt cgacggcgag
     2161 atatcccacg agacgcgcgc cgtcggcctc gcattcgatg tcgctggtct tcacggcacg
     2221 tctcccgaag aaagttatca gtcgtcagtc gtcagtcaag agtaccgcgc gactgacgac
     2281 tgacaactgt a
//
LOCUS       DASYNS010000529        15766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2473152, whole genome shotgun sequence.
ACCESSION   DASYNS010000529 DASYNS010000000
VERSION     DASYNS010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 15766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15766
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2473152"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4166)
                     /locus_tag="VGP50_16330"
     CDS             complement(<1..4166)
                     /locus_tag="VGP50_16330"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEV8029002.1"
                     /translation="MSYGKNGLAPLAFVLFVVTAAAFGAASPGRAEVPDTLKRPANAF
                     VVPEKFLRRWDPVTIFFDRDTGPAKGGAEDDPARFVAMTPAHPGAFTWLDARTLQFRP
                     AEPWPALAHFDWRIGGREMRLVTLLPAPTQTLPADGATALANVESVTLVMPEPVDPAV
                     LAQMITIDLRPLPGLGDAPARTLNGDDFEIKIMERAGRADAAAYVLRFREPIGGGMRA
                     LLHLRLSPESGLEGVTHEIAFATAEPFRAVSFGCPGPRLPVTPTGVTYPEQNALRCDP
                     TNRSLVVQFSAPLGAIGPVVARNLVRITPPVDELTFDSAGDELTITGAFAAETLYRLR
                     LEPSDVQDQDGRALELTQASEVSLFFPAQERFLRWQMSQGIVERLGPQMAPLQGRGFD
                     RLDLRIYPVDPLNRSFWPFPDRPVSTDEAARPPGPGEEPQPVGDQPRQITGHELVEHL
                     KNLGSPAISEIVTTPLHKGGTAARFGLDLKPYFERIAGATAPGTYLVGIRRMDGSAER
                     SWMRLQVTDLSLSTVEEADTVRFFVTSLATGAPVRSAMLHIEGIADNHWTVFGEGHTG
                     EDGQFAWKAPGPPPRGITTLRRVVVEKSGDMLVIDTTRPPPIAGEGAQTTGEQWLQWT
                     VSPHSDRSTPTQLICHLFTERPIYRPEEPVHVKAWLRNWKGGEFSPFTGKTALVLSGG
                     ENVEWRYPLEITQEGTAYHLFDEKTAATGDYEAHLEINGDSCGSVTFKKEAYRLPRFE
                     VQLAAPQKTGLDSAFSVDLAARYYAGGSVAARPLRWRVTQFSYDWQPALREGFVFSSD
                     ARFSTLGQFRSTPVLEREVATDDDGNAKLTIDPTLEPTAQPRRYVVEATVTGEDDQTV
                     TTTQHVLALPPFVLGAKVPRFLEKARSIDPEIIAVGPDGKTIAGQEVTVRLLRRQWHS
                     TLQATDFSQGVAKYVTDIVDDKIAETKIVTEEQAKTLKLPIDRAGVYIVELEAQDRTG
                     RVQTLAVDLFANGDQPLTWAREPAKKFVVANDKSAYAPGETATLVLQSPYQTGAALVV
                     LEHADDRNDYRWVEVRNGAGRIKVDIEKKDMPKLPLHVVLLRGRVGVESTSASPIDMS
                     RPATVMSSTTLAITPVKHQVKVELLAPAQAQPGDEIDLTVNLRDDAGKPLAGEVTLWL
                     VDQAVLALAQEQRLDPLPDFIVERASKLVLRDSRNLVLGLLPLQEMPGGDEGKKEQPL
                     LEQVAIRKNFTPVPYYNPRLMVGPSGSVTLKVKLADSLSNFMLRAKAVSGPDRFGFGT
                     GMIAVRLPVIVEPALPRFVRPGDRFIASAIGRVVEGEGGPGRAEIRVEGLSLKAPAVQ
                     NFNWEKAPKRIEIPVEVPSPGYTASGRLEREAVKLTFGVERSADKAHDAFAVSLPVRP
                     DREPVRRREIV"
     gene            complement(4178..9340)
                     /locus_tag="VGP50_16335"
     CDS             complement(4178..9340)
                     /locus_tag="VGP50_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029003.1"
                     /translation="MQERRPRRLPWVRVFAVMTARRLHTLAVLMLIGAGAALGSGRAA
                     ADETVRQEKIFTVDEAGRYALRLESKAGAALQIIDRMAGPSTLVGEAGKEDGRLDMLL
                     DRGSYKAMMILPKGAAGDVALHIDAFRELNGNAQPRLVELKPVESDLADLTQRSWWID
                     VPSRRTVAIEAAGRYLADLRFWKDGNWLVDVTPSEAVVEREPGHPLAVRRLVAALEPG
                     VYLLTAYGGVGETWAKESAAKPLYLRFGVPMIGEAAQRQRTMSPFGIDRFLVTRPANH
                     FRLQLDHADVAALTVGSYDPGDPFSSSGNRAEIAKNSRDPAVELTAQPTAEFSLVTVE
                     RQPGQPYLLQNFYSAPFYGIDDPGNDWIEVIHSANSDDDADVTGVLTELTTSGERIVA
                     ASAIELAPGQAWRRRFNLLDELTLHVAVAAPGNYRVNAEGENVEAEFRFEPVGPLPEQ
                     YRIPAFERGDHAWALESGIYRLTVRPRPEKKGIATLTIKPAEFTGDIAPARRLGTVAW
                     RSQILSGNTSYRLYTNEVPGISHGLVLRTLPIDLASELPATLAPGEQLELPIHIATQG
                     SIIVEAADGKPITFSLDGHAATDAAQSAAPGAHRIALANASDHPLSLSARFEPDNMRP
                     EAPLPVMSAERLKQRPSLPTLTSEKPVFLDVAQGEQRGFNIAVEAPALYRLETGGLLH
                     TGGNLRTRMVTSLARDDADGVGRNFLIQRYLREGDYQLSFAPQGASAGHLSLAMTATP
                     IEDHGTLTLGIPSRATLRPGLALRYDFRIDHAGLYRLRAFGMNRQFALRAEDADGWPV
                     AQPVVDGVLEQEFEPGLYHATLLPSAAEARSLALLEEIVPAPEYAGHGPHQIALGQHI
                     SHEWLEPEKDGERVPDRWRFTVPASADVTIDLGAGMIATLLRETGGQDTEVARFIRSP
                     GWQGSLAAGDYRLETTSVRPNNHFPYELALRSEELLIGQSREVQSPIALPIALDGNDL
                     VEISSFGDSDVRARLYDAEGRLIAANDDRNNDWNFDIAATLPPGRYRLQVDPVGADEA
                     ATRIDLLRPEEKTEPLLGIPGSAAVADAALHTFALSTPKESGLLILAAQSADAVSLSL
                     ERSADGSLWRSIAGVSGQSSFLAIPVAGEDTAYRLRVWSTDRKSAAIKLTTRFITETP
                     ASESALTSGLSLHRIDGVEPSVWAGALRPARPGVLHIESDSDALRWTSAQGVAAQSDA
                     SALVIAGREPVWLVERGSARAPRLHGVRVDFKDETRLLLPPGEAVIAPASDNAAGPVL
                     WLARSRFGQPGLVVGDIGGALTGMGIAANAGVTVATKAAPLRLWNAEDRSASLQLTLR
                     QTTFDAPASDHFGAGERSLSLAPRTARVLTLDGPGIKRLHLALAPDTVAVLRQDDTTL
                     ATLWPDGQSRSYTTELPADSILLLNAGGEEARVTLAWTEATGTSSALVVAPGQIFKRH
                     DGTAGTLLLHAGTSSPGAATLRIAGNGARGALVAEDGSIRRGNDVALAGHALLMVEHG
                     PGLVAAWIEDEATVVPETALALDGKAQIIRLSGPAMRFAATPQEPVLLSLRTTAPVIA
                     SISAPDGHATTEIFPEGGRISRYLPPGRTVIALQSASEGDLAGDAEFIERLIAPIDEG
                     LGDAVRLAAGETRIYSFALAQEETIGVGLRASVDIAQCRLLDSAGKTLGSGIVQMHKL
                     AAGTYLLAVEMPADSAPVVIRPALVGVRAPEPAPPDEVKRHYLQLVGRLPSQ"
     gene            complement(9892..11028)
                     /locus_tag="VGP50_16340"
     CDS             complement(9892..11028)
                     /locus_tag="VGP50_16340"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEV8029004.1"
                     /translation="MSVANVQPLCPRKRLVGRGVPPAAGRSFARPATPGNRRVLARSR
                     VSCHAPARLHPTPAWKRGNSPAQKALESLIAATAAEHALGNERRLPAPRRRRSALIAG
                     SLALTLIGGVAFKDVGPAGSLSAPEAPLPEVVAGSSGEEAMSGSSTDKAQREVAEASA
                     ELKQIEEDVASATAMPEDVRRAINKATRAVGVDAEYMTVVAARESSFDPEAHAHHTTA
                     SGLYQFTTDTWLRAIKMFGAKHGLSDYAELITIDDDGLVSMSRGAARTDLLSLRSDPE
                     LAALMAAELARDNALRLERMLGRSVTPAETYMAHLFGVVPAARMIGAARSAPHVAGAD
                     LLPTAARSNPSIFNLASGPASAREIVAKIGADFHRHASHSDTSI"
     gene            complement(11333..12868)
                     /locus_tag="VGP50_16345"
     CDS             complement(11333..12868)
                     /locus_tag="VGP50_16345"
                     /inference="COORDINATES: protein motif:HMM:NF028509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEV8029005.1"
                     /translation="MNFLNGLKIKAKLALLLSVSAAALIATLALGASFLHHKMVTDRE
                     VQTRLLVEAALSIIQSSYDRTRTAGVSTEDAQAAAIATVRSMRYGDNGYYFIQRYDGI
                     VVLNPNRPELEGQQRWDAVDPDGVARVRIQIEAAQHGGDFVRYGFPRAGSKDPVQKLS
                     YIAGFEPWRWAIGTGAYVDDIEAEFRSSLLQLGLIASAIFSAALIFAYFINHNVGTSL
                     LWLKDKMERLANGDLTVEIDEAERGDEIGAMAKAMRVFKENAQAAQLLEAEREKRREL
                     EYAMNHADRVDSLGRLASGIAHDLNNALVPVLAMTKSVMLRQPKTSREYANLDLAMMG
                     AQRARELVQQILAFGRKQAIEYRDFDVAQVVNDGIRMLRAVLPSTIKLESAIDPIPTI
                     YGDAGQINQVLVNLVTNSSHAIGDDRGTITVSLRRDDDARVRLSVADNGCGMDDKTAA
                     RLFEPFFTTKEAGKGTGLGLSVVRGIVSSHGGTITVSSAPGQGTRFDVVLPIARQAEA
                     EVA"
     gene            complement(12933..13841)
                     /locus_tag="VGP50_16350"
     CDS             complement(12933..13841)
                     /locus_tag="VGP50_16350"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEV8029006.1"
                     /translation="MYLRPFRYFVAVAEELHFGRAARRMGIAQPPLSRQIRALEGELG
                     VVLFRRTKRDVQLTDVGLVFLAEARAAVERVDRAIARAKQAGRTEHLEIGYTSTVPYS
                     PVFATVIREYRRRRPQIQLSLSEMTTPQQLAALADGKLDVAFVRSPIPDHPKSIALTP
                     LMRERLFVALRDDHDLASAETVALEALDREPFILTPVGTWLRDYVAEMCRTIGFEPQV
                     VQEAQQIVDAIPLVAAGLGVSFVPESACTLAAPGIVFRPLSEAGKSAEIALAYRRSEA
                     SPPVQAFIALAVDAASAGMRPYLRGS"
     gene            14029..15375
                     /locus_tag="VGP50_16355"
     CDS             14029..15375
                     /locus_tag="VGP50_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV8029007.1"
                     /translation="MTGQIDARRHLDGRPALVGLLAENVKTAFEKTFFYGRLFRKQLR
                     GKPITPDLVRELFFGLPFTSKEDVVRNYPNGFLAVPMTSVAAYFESSGTSGNTIHSTR
                     SASFFTRGDLERDVKRRFSPDLAVANGDVVVNALPFALTSSGIGFQQASLAAGALAVT
                     VDSGSMLSSHVKHIELLKELKATVFISSLPLAYSTLLQMEGNDPKAEFASLRAIQLCG
                     LATMANGKAKIRSTFGVPVFDTYGLSEFGATTFTCHAGHMHVHDQDFFFEVINPRDGE
                     LVADGVGGEIVITTLTREGSPKIRYRTGDWGILHFGACTCGRAAPRIEVKGRLRDVAQ
                     FGERFRLPIDFEEIIHRFPATTGLFRLVYEPIEGEGTRLRAALTVDVDDPNRPGLKSE
                     IEEALRAEISPMITVELVRVGGAQVALLDQSKFANVRTVKSAMLDDKRPQEWLVTY"
     gene            15357..>15766
                     /locus_tag="VGP50_16360"
     CDS             15357..>15766
                     /locus_tag="VGP50_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029008.1"
                     /translation="MAGDILEALARQRSANLVETLEHVRAASPLYRRKLADLSLERIV
                     GLADIASLPFTTRGELLELGPFGAVCDGEVAHSYFESTGTSGSFLSGFPDLSAEKARS
                     FGEFLDRWMGLRKDRVRRAIVALAFEMNPTGIRF"
BASE COUNT         2557 a         5103 c         5439 g         2667 t
ORIGIN      
        1 acgatctcgc gccggcgtac cggctcgcgg tcgggcctga cgggcaggct gaccgcgaag
       61 gcgtcatgcg ctttgtcggc gctgcgctcg acgccgaaag tgagcttgac cgcttcgcgc
      121 tcgaggcgtc cgctcgcggt atagcccggc gacggcacct cgaccgggat ttcgatgcgc
      181 tttggcgcct tctcccagtt gaagttctgg accgccggcg ccttgaggct caatccctcg
      241 acgcggatct cggcgcggcc cggcccgcct tcgccttcga cgacgcggcc gatggcgctc
      301 gcgatgaagc gatcgccggg acggacgaag cgcggcagcg ccggctcgac gatcaccggg
      361 aggcgcaccg cgatcatccc ggtgccgaag ccgaagcggt cggggccgct caccgccttc
      421 gcgcgcagca tgaagttgct gagggagtcg gcgagcttga ccttgagcgt gacggagccg
      481 ctcgggccca ccatgagccg cggattatag tagggcaccg gcgtgaaatt cttgcggatc
      541 gccacctgct ccagcagcgg ctgctctttc ttcccttcat cgccgcccgg catttcctgg
      601 agcggcagca gacccagcac gagattgcgc gaatcgcgca gcacgagctt gctcgcgcgc
      661 tcgacgatga agtccggcag cggatcgagc cgctgctcct gcgccagcgc cagcaccgcc
      721 tggtcgacga gccagagcgt gacctcgccc gcgagcggct tccccgcatc gtcgcgcaga
      781 ttgacggtga ggtcgatctc gtcgcccggc tgggcctgcg ccggtgccaa cagctccact
      841 ttcacctgat gctttaccgg cgtgatggcg agcgtcgtgc tgctcatgac cgtcgccggg
      901 cggctcatgt cgatgggcga ggcgctcgtg ctctcgaccc cgacgcggcc gcgcagcagc
      961 accacatgca gcggcagctt cggcatgtct tttttctcga tgtcgacctt gatgcggccg
     1021 gcgccgttcc gcacctcgac ccatcgatag tcgttccggt cgtcggcatg ttcgagcacg
     1081 accagcgccg cgcccgtctg gtacgggctc tgcagcacga gcgtcgcggt ctcgccgggc
     1141 gcataggcgg acttgtcgtt ggcgacgacg aatttcttcg cgggctcgcg cgcccaggtg
     1201 agcggctggt cgccattggc gaagagatcg acggcgagcg tctgaacgcg gccggtgcgg
     1261 tcctgcgcct cgagctcgac gatatagacg ccggcccgat cgatcggcag cttcagagtc
     1321 ttcgcctgct cctcggtgac gatcttggtc tcggcgatct tgtcgtcgac gatgtcggtc
     1381 acgtatttcg cgacgccctg gctgaaatcg gtggcctgca gcgtcgaatg ccattgacgc
     1441 cgcagcaggc gcacggtgac ttcctgaccg gcgatggtct tgccgtcggg accgacggcg
     1501 atgatctcgg gatcgatgct tcgcgctttc tcaaggaagc gcgggacttt ggcgccgagc
     1561 acgaagggcg gcagcgccag cacatgttgc gtggtggtga cggtctggtc gtcctcgccg
     1621 gtgaccgtcg cctcgacgac atagcggcgc ggctgtgcgg ttggctcgag cgtcggatcg
     1681 atagtgagct tggcgttgcc gtcgtcgtcg gtcgcaacct cccgctccag caccggggtc
     1741 gagcggaact gacccaaggt cgagaatcgg gcatcgctcg agaacacgaa gccttcgcgc
     1801 aaggccggct gccagtcata ggagaattgc gtcacgcgcc agcgcagggg acgggcggcg
     1861 acgctgccgc cggcgtagta gcgggcggcg aggtcgacgc tgaaggcgct gtcgagcccg
     1921 gtcttctgcg gcgccgcgag ctgcacctcg aagcgcggca ggcgataggc ctctttcttg
     1981 aaggtgacgc tgccgcagct gtcgccgttg atctcgagat gcgcctcgta gtcgccagtc
     2041 gccgccgtct tctcgtcgaa gagatgatag gccgtgcctt cttgcgtgat ctcgagcgga
     2101 tagcgccatt cgacattctc gccgccgctg aggacgagcg ccgtcttgcc ggtgaatggg
     2161 gagaactcgc cgcccttcca gttgcgcagc caggccttta catggaccgg ctcctccggc
     2221 cggtagatcg gccgctcggt gaagagatgg cagatcagct gcgtcggcgt cgaccggtcg
     2281 gaatgcggcg agaccgtcca ttgcagccat tgctcgcccg tggtctgtgc cccctcgccg
     2341 gcgatcggcg gtggccgcgt cgtgtcgatg acgagcatgt cgccggattt ctcgacgacg
     2401 acgcgccgca gggtcgtgat gccgcgcggc ggcggcccgg gagccttcca agcgaattgt
     2461 ccatcctcgc cggtgtggcc ctcgccgaag accgtccaat ggttgtcggc gatgccctcg
     2521 atgtgcagca tcgcgctgcg caccggcgcg ccggttgcga gcgaggtgac gaagaatcgc
     2581 acggtgtcgg cttcctcgac ggtgctgagg ctcagatccg tgacctggag ccgcatccaa
     2641 ctgcgctcgg cgcttccatc catccgccgg atgccgacga gatatgtccc gggtgccgtg
     2701 gcgccggcga tgcgctcgaa atagggtttg agatcgaggc cgaagcgcgc cgccgttccg
     2761 cccttgtgca gcggcgtcgt gacgatctcc gagatcgctg gcgacccgag atttttcaga
     2821 tgctcgacga gctcatgccc ggtgatctgg cgcggctgat cgccgacggg ctggggctcc
     2881 tcgccggggc cgggcggccg tgccgcttcg tcggtcgata cgggacggtc gggaaagggc
     2941 cagaaggacc gattgagcgg gtcgacggga tagatgcgca agtcgagacg atcgaagccg
     3001 cggccctgga gcggcgccat ttgtgggccg aggcgctcga cgatgccttg gctcatctgc
     3061 cagcgcagga agcgctcctg cgcgggaaag aacagcgaaa cttcgctcgc ttgcgtgagc
     3121 tccaaagcgc gcccgtcctg gtcctggaca tcgctcggct cgagcctgag gcgatagagc
     3181 gtctcggcgg cgaaagcgcc ggtgatggtg agctcgtcgc cggcgctgtc gaatgtcagc
     3241 tcgtccaccg gcggggtgat ccgcaccagg ttgcgcgcca ccaccgggcc gatggcgccg
     3301 agcggcgcag agaactgcac gaccagcgag cggttggtgg gatcgcagcg gagcgcattc
     3361 tgctcggggt aggtgacgcc ggtcggcgtc accggcaggc gcggccccgg acagccgaaa
     3421 ctcaccgcgc ggaagggttc ggcggtggcg aaggcgatct cgtgcgtgac gccctccagc
     3481 cccgattccg gcgagagccg cagatggagg agcgcgcgca tgccgccgcc gatgggctcg
     3541 cggaagcgca gaacataggc cgcggcgtcg gcgcgtccag cgcgctccat gatcttgatc
     3601 tcgaagtcgt cgccattgag cgtgcgtgcc ggagcgtcgc cgagcccggg gagcggacgc
     3661 aggtcgatgg tgatcatctg cgccagcacg gcgggatcga ccggctccgg catcaccagc
     3721 gtcacgctct cgacattggc gagcgcagtc gcaccgtcgg ccggcaaagt ctgcgtcggc
     3781 gccggcagca gcgtgacgag ccgcatttcg cgcccgccga tgcgccaatc gaaatgcgcc
     3841 agggccggcc agggctcggc cggccggaac tggagcgtgc gcgcgtcgag ccaggtgaag
     3901 gcgcccggat gggccggcgt catggcgacg aagcgcgccg ggtcgtcctc tgcgccgccc
     3961 ttcgccgggc ccgtgtcacg gtcgaaaaag atcgtgacgg ggtcccagcg ccgcagaaat
     4021 ttctccggaa cgacgaaggc attggccggc cgcttcagcg tgtccggcac ctccgcgcgg
     4081 ccgggcgatg cggcgccgaa agcggcagcg gtgacgacga acaggacgaa cgccagaggc
     4141 gcgagcccgt tcttgccgta cgacatggaa gcaactccta ttgggaagga agtctgccga
     4201 cgagctgcag gtagtgccgc ttgacctcgt cgggaggcgc aggttcgggc gcgcgcaccc
     4261 cgacaagcgc cggacggatg acgacgggag ccgaatcggc cggcatctcc acggcgagca
     4321 aatacgtgcc ggccgccagc ttgtgcattt gtacgatacc ggagcccagc gtcttgccgg
     4381 cgctgtcgag gagacggcat tgtgcgatgt cgacagaagc gcgcaggccg acgccgatcg
     4441 tctcctcctg cgccagcgcg aaggaataga tgcgagtctc gccggcggcg agccgcaccg
     4501 catcgccgag cccttcgtcg atcggcgcaa tcagccgctc gatgaattcg gcatcgcccg
     4561 cgaggtcgcc ttcgctggcg gattgaagcg cgatcaccgt gcgtcccggc gggagatagc
     4621 ggctgatgcg tccgccttcg gggaagatct cggtcgtcgc gtggccgtcg ggcgcgctga
     4681 tgctggcgat aacgggcgct gttgtgcgca ggctcagcag caccggctcc tgtggcgtcg
     4741 ccgcgaagcg catcgccgga cccgagaggc ggatgatctg cgctttgccg tcgagcgcca
     4801 acgcggtctc cggaacgacc gtcgcctcgt cctcgatcca ggccgcaacg agaccggggc
     4861 cgtgctcgac catcagcagg gcatggccgg cgagagcgac gtcattgccg cggcgaatgc
     4921 tgccgtcttc ggcgacaagc gcgccgcgtg cgccattgcc ggcgatgcga agcgtggcgg
     4981 cgccgggcga cgacgttccg gcgtggagca ggagcgtccc ggccgtgccg tcatgacgct
     5041 tgaagatctg gccgggcgcc acgaccagcg ccgaggacgt gcccgtcgcc tccgtccagg
     5101 ccagtgtcac gcgggcctcc tcaccgccgg cgttgagcag caggatcgag tcggcgggga
     5161 gctccgtcgt atagctgcgg ctctgtccat cgggccataa ggtcgcgagt gtcgtgtcgt
     5221 cctggcgcag caccgcgacg gtgtcgggcg ccagcgccag atgcagtcgt ttgatgcccg
     5281 gcccgtcgag cgtaaggacg cgcgccgttc gcggagcgag cgacaagctg cgttcgcccg
     5341 cgccgaaatg atcgctggcg ggcgcgtcga acgttgtctg acgcaaggtc agttggagcg
     5401 acgccgaccg gtcttcggca ttccacagtc gcagcggcgc ggccttcgtc gcaacggtga
     5461 cgccggcatt ggcggcgatg cccatccccg tgagcgcgcc gccgatatcg cccacgacca
     5521 ggcccggctg tccgaagcgc gagcgcgcga gccacaggac cggcccggca gcattgtccg
     5581 aggccggagc gatcacggct tcgccgggcg gcagcagcaa ccgcgtttca tccttgaagt
     5641 cgacgcgcac gccgtgcagc cggggcgctc tggcgctgcc gcgctcgacg agccagacgg
     5701 gttcgcgacc ggcgatgacg agcgcggagg cgtcgctctg cgcggcgacc ccctgcgcgc
     5761 tcgtccagcg cagcgcatca ctgtcgctct cgatgtggag gacgccgggg cgcgctggtc
     5821 tgagggcgcc ggcccaaacg gacggctcga cgccatcgat ccggtgcagg gacagaccgc
     5881 tcgtcagcgc gctttcgctg gccggcgtct cggtgatgaa gcgggtcgtc agcttgatcg
     5941 cggcgctctt ccgatcggtg gaccagacgc gcaagcgata ggccgtgtcc tcgccggcga
     6001 cagggatggc caagaaagaa gattgccccg agacgccggc gatgctgcgc caaaggctac
     6061 cgtcggccga gcgctcgagc gagaggctca ccgcgtccgc ggactgcgcc gccagtatca
     6121 gcaggccgct ctccttgggc gtgctcagcg caaaggtatg gagcgcggca tccgcgaccg
     6181 cggcgctgcc gggaatcccc agcaatggtt cggtcttctc ctccggtcgc aagaggtcga
     6241 tgcgcgtcgc agcctcgtcc gcgccgacgg gatcgacctg caagcgatag cgtcccggcg
     6301 gcagcgtcgc ggcgatgtcg aaattccagt cgttgttgcg gtcgtcattg gcggcgatca
     6361 gccggccctc ggcatcgtag aggcgggcgc gcacgtcgct gtcgccgaag gagctgatct
     6421 cgacgaggtc gttgccatcg agcgcgatcg gcagagcgat cggcgactgt acctcccggc
     6481 tctgcccgat gaggagctct tcggagcgca gcgccagctc gtagggaaag tgattgttcg
     6541 gccggacgct cgtggtttcg agccgataat cgccggcggc gaggctgccc tgccagccgg
     6601 ggctgcggat gaagcgcgcg acctccgtgt cctgaccgcc cgtctcgcgc agcagcgtcg
     6661 cgatcatgcc ggcgccgaga tcgatggtga catcggccga cgcgggaacc gtgaagcgcc
     6721 agcggtcggg aacgcgttcg ccgtccttct cgggctcgag ccattcatgg gagatgtgct
     6781 ggccgagggc aatctgatgt ggcccatgtc ctgcgtattc gggcgcgggc acgatctctt
     6841 cgaggagagc gagggaacgc gcttcggccg cgctcggcag cagcgtcgca tggtaaagcc
     6901 cgggctcgaa ttcttgctcg agcacgccgt cgaccaccgg ctgggccacg ggccagccat
     6961 cggcgtcctc ggcccggagg gcgaattggc ggttcatgcc aaaggcgcgc aagcggtaga
     7021 gcccggcatg atcgatccgg aaatcgtagc gcagcgcgag acccggccgg agagtcgccc
     7081 gcgacgggat gccgagggtc agcgtgccgt ggtcctcgat cggtgtcgcg gtcatggcga
     7141 ggcttagatg accggccgaa gcgccttggg gcgcgaagct cagctgataa tcgccttcgc
     7201 gcagatagcg ctggatgagg aaattgcggc cgacgccatc ggcgtcgtca cgggccagcg
     7261 acgtgaccat gcgcgtgcgc agattgcccc cggtatgcag cagcccgccg gtctcgagcc
     7321 gatagagggc cggcgcttcc acggcgatgt tgaagccgcg ctgctctccc tgcgccacgt
     7381 cgaggaacac cggcttctcg gacgtgagcg tcggcaagga tgggcgctgt ttcagacgct
     7441 ccgccgacat cacgggcagc ggtgcttcgg gtcgcatgtt gtcgggctcg aagcgcgccg
     7501 agagcgacag cggatgatcg ctcgcgttgg cgagcgcgat gcggtgcgcg ccgggcgccg
     7561 cgctctgagc ggcatccgtc gccgcatggc cgtccagaga aaaggtgatc ggttttccgt
     7621 ccgccgcctc gacgatgatc gagccttgcg tcgcgatgtg gatgggcaac tcgagctgct
     7681 cgcccggcgc cagcgtcgcc ggcaattcgc tcgcgaggtc gatcggcagc gtgcggagca
     7741 ccagaccgtg actgatgccc ggcacctcgt tggtgtagag ccggtagctc gtgttgccgg
     7801 agaggatctg gctgcgccac gcgacagtgc cgaggcgccg cgccggtgcg atatcgccgg
     7861 taaattccgc cggctttatc gtgagcgttg cgatgccctt cttctcgggc cgcggcctga
     7921 cggtcagccg atagattccg ctttcgagcg cccacgcatg gtcgccgcgc tcgaaggcgg
     7981 ggatgcgata ttgctcgggg agcggcccga cgggttcgaa gcggaactcg gcctcgacat
     8041 tctcgccttc ggcattcacc cgatagttac cgggcgccgc caccgccaca tgcagcgtca
     8101 gctcgtcgag caggttgaag cggcggcgcc aggcctgccc cggcgccaat tcgatggcgc
     8161 tcgcggcgac gatgcgctcc cctgacgtgg tgagctcggt gagcacgccg gtgacgtcgg
     8221 catcgtcgtc gctgttggcg ctatggatca cctcaatcca gtcgttgccg ggatcgtcga
     8281 tgccatagaa aggcgcgctg tagaagtttt gcagcaggta gggctgaccg ggttggcgct
     8341 cgacggtgac gagcgagaac tcggcggtcg gctgtgccgt cagctcgacg gcgggatcgc
     8401 gactgttctt ggcgatctcg gcccggttgc cgctgctgct gaaggggtcg ccgggatcgt
     8461 agctgccgac tgtcagcgcc gccacatcgg catggtcgag ctgcagccgg aaatgattgg
     8521 cggggcgcgt tacgagaaag cgatcgatgc cgaaaggact catcgtccgc tgtcgctgcg
     8581 cagcctcgcc gatcatcgga acgccgaagc ggagataaag cggcttcgcc gccgattcct
     8641 tggcccaggt ctcgcccacg ccgccatagg cggtcagcag gtagactcca ggctcgagcg
     8701 ccgccacgag gcggcggacg gcaagcgggt gccccggctc ccgttcgacg accgcctcgc
     8761 tgggcgtgac gtcgaccagc caattgccgt ccttccaaaa acgcagatcg gcgaggtagc
     8821 gtccggccgc ttcgatggcc acggtccgtc gcgacggcac gtcgatccac caggagcgct
     8881 gcgtcaggtc ggcgagatcg ctctcgaccg gtttcagctc gacgaggcgc ggctgggcgt
     8941 tgccgttgag ctcgcggaac gcgtcgatat gaagagccac gtcgccggcg gcgcctttgg
     9001 gaagaatcat catggccttg tagctgccgc gatcgagcag catgtcgagg cgcccatctt
     9061 cttttccggc ctcgcccaca agggtcgaag gtcccgccat gcgatcgatg atctggaggg
     9121 ccgcccccgc tttgctttcg aggcggagcg cataacggcc ggcctcgtcg accgtgaaga
     9181 ttttttcctg gcgaacggtc tcgtccgcgg cggcacggcc ggagcccaat gcggcgccgg
     9241 caccgatcag catcaggacg gcgagtgtgt gaagacgcct cgccgtcatc accgcgaaaa
     9301 cgcggaccca gggcaagcgg cgcggccgtc gctcctgcat ttccgattgc gcgggaatga
     9361 ttattttgaa tagcgcggat tccgttcggg cgaaatccgt tcggcggcgg cgagcattgc
     9421 gtcgcgcgcg caagagcaga cgagcgaagg aggggagcaa ggaagcaccg aagagaagat
     9481 cgagcgccgc aatggcagca tccatctttg tctatttcaa ggcgaagatg cgcgtgatgc
     9541 gcatgtgtga gtcgcggcgg agggaagacg cgcaaagctg gcacgttccg atcgcaggga
     9601 gcttgaatgc gactggccca agtgaacggt gaataaaccg cggccagcgc catccgctga
     9661 gtccagggga atacctgagc gcgcgcgaga tgctgcttca ggcggtggtc cggaaatgcg
     9721 gcatgccgcg gaaacgcgct cttcgcggcg gaccggcaag caggtgcgct cgctgccgct
     9781 caaagaaatg ccgcttcgcg acggcctcag gattcggcga aacgcaaagg aaaaggtgcc
     9841 gccgagatcc ccggcggcac cttgccttgc gccactaatt gctggatcgg cttagatgct
     9901 cgtgtcgcta tgcgacgcgt ggcggtggaa atcggcgccg atcttggcga cgatctcgcg
     9961 ggcggaggcg ggcccgcttg ccaggttgaa gatgctgggg ttgctgcgcg ccgccgtcgg
    10021 caggagatcg gcgcctgcga catgcggggc ggagcgcgcc gcgccgatca tgcgggccgc
    10081 cggcacgacg ccgaagagat gcgccatata ggtctccgcc ggggtgacgc tccggccgag
    10141 catgcgctcg aggcgaaggg cgttgtcgcg ggccagctcg gccgccatca gcgccgcaag
    10201 ctccgggtcg ctgcgcaggc tgaggaggtc cgtgcgcgcg gcgccccgcg acatcgagac
    10261 gagcccgtca tcgtcgatcg tgatgagctc ggcatagtcg gagagcccgt gcttcgcgcc
    10321 gaacatcttg atggcgcgca gccaggtgtc agtggtgaac tggtagaggc cggacgccgt
    10381 ggtgtggtgg gcatgggcct cggggtcgaa gctcgattcc cgggccgcaa ccaccgtcat
    10441 atactcggca tcgacgccga cggcgcgggt ggctttgttg atggcgcggc gcacgtcttc
    10501 gggcatcgcg gtcgccgagg cgacgtcttc ttcgatctgc ttgagctcgg cgctggcttc
    10561 tgcgacctcg cgttgggcct tgtcggtgct cgagccggac atggcttctt cgccgctcga
    10621 cccggcgacc acttccggaa gcggcgcctc cggggcggag agggacccgg cgggaccgac
    10681 atccttgaag gcgacgccgc cgatcagggt caaagcgagg gagccggcga tcagggccga
    10741 gcgccgccgg cgcggagcgg ggaggcgccg ctcgttgcct agggcgtgct cggccgctgt
    10801 cgcggcaatg agggattcga gggctttctg cgccgggctg ttcccgcgct tccaggcggg
    10861 cgtcgggtgc agccgcgccg gcgcatggca ggagacgcgg gagcgagcga ggacccgccg
    10921 gttcccagga gtggccggcc tggcaaagct gcggcctgca gccggcggta ccccacgacc
    10981 gaccaatctc ttgcgggggc agagcggctg gacattggct accgacatgg tccctccttg
    11041 gaaggcgccg agttggtggc aagcggccac ccggcagatg ggagagatta gagacgattt
    11101 ctcggagtaa tgccaggagt attttcgtca ctatttagta caattttagc agaagtttcg
    11161 atatctccta cctttgtctg tcacggttct caattaatat taatggttaa atgatggcaa
    11221 ggtcacagtt aagccagaca tcggtcagga ctgtgcttgt cctgttacaa tcacagcggc
    11281 gagtcgactt atatatgggc tatatcagcg tgtcggcgag gatcgatcgg cctcaagcga
    11341 cttccgcttc ggcctgccgg gcgatcggca ggacgacgtc gaaccgcgtg ccttggccgg
    11401 gcgcgctgct gacagtgatg gtgccgccat gggagctgac gatgccgcgc accaccgaga
    11461 ggccaagccc ggttcccttg ccggcctctt tggtggtgaa gaacggctcg aagaggcggg
    11521 cggccgtttt gtcgtccatg ccgcaaccgt tgtccgcgac cgagagacgg acccgggcgt
    11581 cgtcgtctcg cctgagactg acggtgatgg tcccccggtc gtcgccgatc gcatgcgagc
    11641 tgttggtcac gagattgacc agcacttggt tgatctgacc ggcatcgccg tagatggtgg
    11701 ggatcgggtc gatcgcgctc tcgagcttga tcgtgctcgg caggacggcg cgcagcatcc
    11761 ggataccgtc attgaccact tgcgcgacgt cgaagtcgcg atactcgatc gcctgctttc
    11821 tgccgaaggc gaggatctgc tgcacgagtt cgcgcgcccg ctgggcgccc atcatggcga
    11881 gatcgagatt ggcgtattcg cggctggtct tgggctgccg caacatcacc gacttcgtca
    11941 tcgccagcac gggcaccagc gcattgttca aatcatgggc gatgccgctg gcgagccgcc
    12001 ccagcgaatc cacgcggtcg gcgtggttca tggcgtattc gagctcgcgg cgcttctcgc
    12061 gctcggcctc aaggagctgc gccgcctggg cgttctcctt gaagacgcgc atcgccttcg
    12121 ccatcgcgcc gatctcgtcg ccgcgctcgg cttcgtcgat ctcgacggtg aggtcgccgt
    12181 tggccagccg ctccatcttg tccttgagcc agaggagcga ggtgccgaca ttgtggttga
    12241 tgaaatacgc gaagatcagc gcggcgctga agattgcgct cgcgatcaga ccgagctgga
    12301 ggagagacga acggaactcg gcctctatgt catcgacata tgcaccagtg ccgattgccc
    12361 atctccaggg ctcgaacccc gcgatatagg acagcttttg cacggggtcc ttgctgccgg
    12421 cgcgcgggaa gccgtagcgc acgaagtcgc cgccgtgctg cgccgcctcg atctgaatcc
    12481 gcacgcgagc aacgccgtcc gggtcgacgg cgtcccatcg ctgttgtccc tcgagctccg
    12541 gccggttggg attgagcacg acgatcccgt cgtagcgctg gatgaaatag tagccgttgt
    12601 cgccgtagcg catcgaccgc acggtcgcga tcgcggcggc ttgcgcgtct tccgtgctca
    12661 cgcccgccgt ccgcgtccga tcgtaggagc tttggatgat gctcagcgcc gcctcgacca
    12721 ggaggcgcgt ctgcacctcg cgatccgtta ccatcttgtg gtgaaggaac gaagcgccga
    12781 gcgccagcgt ggcaatgagc gccgccgccg aaaccgacag caaaagggcg agcttcgcct
    12841 tgatcttgag gccgttcaga aaattcacgg caaactttgc cccaaatggg tccgaaatcg
    12901 ttgcgggaat tcaccataat ggggaattcc ggctaagaac cccttaaata tgggcgcatt
    12961 cccgcggatg ccgcgtcgac ggccagcgcg atgaaggcct ggacaggagg cgaggcttcg
    13021 ctccgccgat aggcgagcgc gatctccgcc gacttgcccg cctccgacag cggccgaaag
    13081 acgatgccgg gcgcggcgag cgtgcaggcc gattccggga cgaaggacac gccgaggccg
    13141 gcggcgacga gcggaatcgc gtcgacgatc tgctgcgcct cctgcacgac ctgcggctcg
    13201 aagccgatgg tccggcacat ctcggcgaca taatcgcgca gccaagtgcc caccggcgtg
    13261 agaatgaagg gctcgcggtc gagcgcctcg agcgccaccg tctcggcgct cgccaagtca
    13321 tggtcgtcgc gcaacgcaac gaaaagacgc tcgcgcatga gcggcgtgag cgcgatggat
    13381 ttggggtggt ccgggatcgg cgagcgcacg aaggcgacgt cgagcttgcc gtcggcaagg
    13441 gcggcgagct gctgcggcgt cgtcatctcg ctgaggctga gctggatctg cgggcgccgg
    13501 cgccggtatt cgcggatcac cgtcgcgaag accggcgagt aaggcacggt gctggtgtag
    13561 ccgatctcga gatgctcggt ccggcccgct tgcttggcgc gggcaatcgc gcgatcgacc
    13621 cgctcgacgg ccgcccgcgc ctcggccagg aacacgagac ccacatccgt cagctgcaca
    13681 tcgcgcttcg tgcggcgaaa gaggacgacg cccagctccc cctcgagcgc gcgaatctgt
    13741 cggctcaagg gcggctgggc gatgcccatc cgccgggcgg cgcggccgaa atgcagctct
    13801 tcggcgacag cgacgaaata gcggaacgga cgcaagtaca tagtctgata cctaaaaggt
    13861 aattgtaatc gccaataaag tattggattt gtgttcttac cgcaacaaat aattgcagga
    13921 agaattcgct tgtgtgtaga tttcagcagc ttcttacgcg gagcttacag aatgggacac
    13981 atttcaagaa ctttggccgt tggccagacc gccgtcgctg atcgcgagtt gaccgggcag
    14041 atcgatgcgc ggcgtcatct cgacggccga ccggcactcg tgggactgct cgccgagaac
    14101 gtcaaaacgg ctttcgagaa gacgtttttc tatggccggc tcttccgcaa gcagcttcgc
    14161 ggcaagccga tcacgcccga cctcgttcga gaattgttct tcggcctgcc cttcacctcg
    14221 aaggaggatg tggtccgcaa ctatccgaac ggcttcctgg ccgtgccgat gacgtccgtc
    14281 gccgcctatt tcgaatcctc cggcacgagc ggcaacacca ttcacagcac acggagcgcg
    14341 tccttcttca cccgcggcga tctcgagcgc gacgtcaagc ggcgcttttc gccggacctc
    14401 gcggtggcga acggcgatgt cgtcgtcaac gccctgccct ttgcgctcac cagctccggc
    14461 atcggcttcc agcaagcctc gctcgcggcg ggtgctctgg ccgtgacggt cgacagcggg
    14521 agcatgctga gcagccacgt caagcacatc gagctcctga aggagctcaa ggccaccgtc
    14581 ttcatcagct cgctaccgct cgcttacagc accttgctgc agatggaggg caacgacccg
    14641 aaggccgagt tcgcgagtct gcgcgccatc cagctctgcg gccttgccac catggccaac
    14701 ggcaaggcga agatccgcag caccttcggc gtgccggtct tcgacaccta cgggctctcg
    14761 gaattcggcg ccacgacctt cacctgtcat gccggccaca tgcacgtcca tgaccaggac
    14821 ttcttcttcg aggtcatcaa tccgcgcgac ggcgagctcg tggccgacgg cgtcggcggc
    14881 gagatcgtga tcaccacgct gacgcgcgag gggtcgccca agatccgcta ccgcaccggc
    14941 gactggggca tcctgcattt cggcgcatgc acatgcggac gtgccgcgcc gcgcatcgag
    15001 gtcaagggac gactccgcga cgtcgcgcag ttcggcgagc gcttccgcct gccgatcgac
    15061 ttcgaggaaa tcatccaccg cttcccggcg acgaccggtc tcttccggct cgtctatgag
    15121 ccgatcgagg gcgaaggcac gcgcttgcgc gcggcgctca ccgtcgatgt cgacgatccg
    15181 aaccggcccg gactcaagtc cgagatcgag gaggcgttgc gcgccgagat ctcgccgatg
    15241 atcaccgtcg agctcgtgcg cgtcggcggc gcgcaagtcg ccctgctcga tcagagcaag
    15301 ttcgccaacg tgcgcaccgt caaatccgcc atgctcgacg acaagaggcc gcaagagtgg
    15361 ctggtgacat actagaggcg ctggcgcggc agcgctccgc gaatcttgtc gagacgctcg
    15421 agcacgtccg cgcggcctcg ccactctatc gccgcaagct cgccgatctc agcctcgagc
    15481 ggatcgtcgg gctcgcggat atcgcgtcgc tgccgttcac gacgcgcggc gagctcctcg
    15541 agctcggtcc gttcggcgcg gtctgcgatg gcgaggtcgc ccattcctat ttcgaatcga
    15601 ccggcaccag cggctcgttc ctgtcgggat tccccgatct ctcggccgag aaggcccgga
    15661 gcttcggcga gttcctcgac cgctggatgg gcttgcgcaa ggatcgcgta cgccgcgcca
    15721 tcgtcgcgct cgccttcgag atgaatccga cgggcatccg cttcca
//
LOCUS       DASYNS010000530         5310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2003984, whole genome shotgun sequence.
ACCESSION   DASYNS010000530 DASYNS010000000
VERSION     DASYNS010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003984"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..777)
                     /locus_tag="VGP50_16365"
     CDS             complement(73..777)
                     /locus_tag="VGP50_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8029009.1"
                     /translation="MLEVTDLVVHYGKALALEGVSLTVGVGELVAVLGPNGAGKSTLL
                     KAISRAVPMSRGHIVFNGKPLDPLPAHKVVAAGICHCPEGRRLFPELTVLKNLQLGAY
                     LRDDKAEIAADLERVYALFPVLKERSRQQASTLSGGEQQMVAIGRALMGRPKLLLLDE
                     PSVGIAHRLKVQIFHSIREIQRAGTAILLVEQDALSALAIAGRVYVMEHGRIAREGTS
                     ADLSRDDYIRQVYLGV"
     gene            complement(770..1513)
                     /locus_tag="VGP50_16370"
     CDS             complement(770..1513)
                     /locus_tag="VGP50_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8029010.1"
                     /translation="MTALLDVAGVTKRFGGLVAVKDVSFALEAGEITGILGPNGAGKT
                     TLFNLLTGFMATDRGIVRFEGRALADLPPHRIVNLGLARTFQLCRPFRGMSVLENASV
                     GCLAPRVRHEGSVTARAQRILEQVGLADKAATLVDVLPYGDLRRLEIARALATRPKLL
                     LLDEPFAGLGSNEIGPLSALIRKLHAEEGLTILIIEHKLREFMQLVRRVIAMDFGEVI
                     AIGPPQEIVRHPRVVEAYIGRTEAPLGLA"
     gene            complement(1510..2436)
                     /locus_tag="VGP50_16375"
     CDS             complement(1510..2436)
                     /locus_tag="VGP50_16375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8029011.1"
                     /translation="MRRLNKDVVGAVAVLVLLAMAPLVVRSDYFSGILTVSVIYAIWA
                     ASWDFMSGLTGRENFGHALFIGAGAYTAAFLNSKLDLSPWWGLPAGALVAVAFSLVVG
                     FPTLRLRGPYFALAMLAAATIMQRLTRIFWQYTGGEEGIPGLDPLFESHLATYYFCLA
                     ALVLASVLLLFLARSSWGTILRAIRGDEAACQAAGINVTFYKIAALMISAAVAGAGGA
                     LYADYQEAVTPDVFAVSLSITLIIMVYVGGMGTIWGPVGGAILLAMLPELLRGLGEYR
                     LWVYSVVLIFILFYSPGGLVAPLWRWLTRPSS"
     gene            complement(2445..3299)
                     /locus_tag="VGP50_16380"
     CDS             complement(2445..3299)
                     /locus_tag="VGP50_16380"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8029012.1"
                     /translation="MLNILVYGAVTSAIYAMLAVGFTLIFGVARILNLSHGAFYGLGA
                     YAAYVMTGLLHWPLPLAAVVAVGFVAAFGAAMERLLVRPLRSSPLAVLMITLAVALIF
                     EQGMFLLFGSEARNVPAFVTETCACAGLDISGQRLLTLGAGMAVLALLYVFIQKTRLG
                     SAILAISQDPEGAQYMGIPANRIYSVVMGLSAAIAALAGVLTAPFLTVQPTMDLLPMV
                     KAFAIVIVGGLGSIPGSILAALLLGYSETVVSYGISPAWTEIVSLIAVLLTLILRPAG
                     LLGKRAAF"
     gene            complement(3449..4675)
                     /locus_tag="VGP50_16385"
     CDS             complement(3449..4675)
                     /locus_tag="VGP50_16385"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8029013.1"
                     /translation="MHRPALCCALATLALGATAALADEKPLIIGATAAIQLQVGRDTV
                     DALQLGIDEINAKGGVLGRKLALAVEDETMDPQQGVAAVKKLTTDDHVDVLIGGYSSG
                     VTLAQEPHIADAKTIWMGVGAASPAITDFVGKNYNRYKYVFRVNPINAVRQGEQVAGF
                     VNSNVKGEFGYDTIAIVGENAKWVQDLAPVLKENVEKGGVKVAMLDLFDPDMSDFSPL
                     LSKVKESGAQYLVVILSHANSDVFVKQWYDAKFPMPIGGIDVKSQDPDFFTRIGGKSV
                     SETGGMTFFAAPLTPKTMPFWNAFKDRYQRLPVYTAPGAYDALHIYVEAVERAGTTDA
                     EKVIPELEKTKYMGTQGTYVFDEHHDVLPGPDLLNLIFVQWQPDGNRAIVWPKELASG
                     KMILPPWVSQSAEAAK"
     gene            complement(4771..>5310)
                     /locus_tag="VGP50_16390"
     CDS             complement(4771..>5310)
                     /locus_tag="VGP50_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029014.1"
                     /translation="HMNGDAGVFLGNGQVSALLEQLAPINVLGALGVYVTGDRPVQIN
                     CMVSRFDIKAGVATASTLLVDTKAVEIVGKGNLNFADETLDLNLEPYNKGPTVVSLRM
                     PVDITGTFAKPDYHLETATLVARLGAAVGLGVLFPPAALAPLVDIGLGDANACSKAYA
                     AQNPPGSPTPTAGSSTPKR"
BASE COUNT          959 a         1638 c         1909 g          804 t
ORIGIN      
        1 gtatgggcca ggctcgatat tcctcatcct gagcttgtcg aaggacgcac ggccgcgatg
       61 cagcctggcg cctcacacgc cgagataaac ctggcggatg tagtcgtcgc gggagaggtc
      121 ggcgctggtg ccttcgcggg cgatgcggcc gtgctccatg acatagacgc ggccggcgat
      181 cgccaacgcc gagagcgcgt cctgctcgac caggaggatg gcggtgccgg cgcgctggat
      241 ctcgcggatc gagtggaaga tctgcacctt gagacgatgg gcgatgccga cggaaggctc
      301 gtcgagcagc aagagcttcg ggcgtcccat caaggcgcgg ccgatcgcca ccatctgctg
      361 ctcgccgccc gagagcgtgc tcgcctgctg gcgcgagcgc tccttgagca cggggaagag
      421 cgcatagacg cgctcgaggt cggcggcgat ctcggccttg tcgtcgcgga gataggcgcc
      481 gagctgaagg ttcttgagca cggtgagctc ggggaagagg cggcggcctt cggggcagtg
      541 gcagatgccg gcggcgacca ccttgtgcgc gggcagcgga tcgagcggtt tgccattgaa
      601 gacgatgtgc ccgcggctca tcggcaccgc gcgcgagatc gctttgagca gcgtcgactt
      661 gccggcgcca ttcgggccga gcacggcgac gagttcgccc acgccgacgg tgaggctcac
      721 gccttcgagc gccagggcct tgccgtaatg cacgacgagg tcggtgacct caagcaaggc
      781 caagcggcgc ctccgtccgg ccgatatagg cttcgacgac gcgcgggtgg cgcacgatct
      841 cctgcggcgg cccgatggcg atgacctcgc cgaaatccat ggcgatgacg cggcgcacga
      901 gctgcatgaa ctcgcgcagc ttgtgctcga tgatgaggat ggtgaggcct tcctcggcat
      961 ggagcttacg gatgagcgcc gaaagcggcc cgatctcgtt gctgccgaga cccgcgaagg
     1021 gctcgtcgag gagcaagagc ttgggccggg tcgcaagcgc gcgggcgatc tcgagccggc
     1081 gcaggtcgcc ataagggagg acgtcgacca gcgtcgctgc cttgtcggcg aggcctacct
     1141 gctcgaggat gcgctgtgcc cgcgccgtga cggagccctc atggcgcacg cgcggcgcga
     1201 ggcagccgac gctcgcattc tcgagcacgc tcatgccgcg gaagggacgg cagagctgga
     1261 acgtgcgcgc gaggccgaga ttgacgatgc gatggggcgg caagtcggcg agcgcgcgtc
     1321 cctcgaagcg gacgatgccg cggtccgtcg ccatgaagcc ggtcagcagg ttgaagagcg
     1381 tcgtcttgcc ggcgccgttg gggcccagga tgccggtgat ctcccccgcc tcgagcgcga
     1441 aggacacgtc cttgaccgcg acgaggccgc cgaagcgctt ggtgacgccc gcgacgtcga
     1501 gcagtgccgt catgaggagg gccgcgtgag ccagcgccag aggggtgcca cgagcccgcc
     1561 ggggctgtag aacaggatga agatcagcac gacactgtag acccagagcc ggtactcgcc
     1621 gaggccgcgc agcagctccg gcagcatcgc cagcaggatc gcgccgccga ccggccccca
     1681 gatcgtgccc atgccgccga catagaccat gatgatgagg gtgatggaca gcgaaacggc
     1741 gaagacgtcg ggcgtcaccg cttcctggta gtcggcgtag agcgcgccgc ccgcgccggc
     1801 gacagcggcg ctgatcatca gcgcggcgat cttgtagaag gtgacgttga tgccggcggc
     1861 ctggcacgcg gcctcgtcgc cgcggatcgc gcgcaggatg gtgccccagc tcgagcgcgc
     1921 caggaaaagc agcagcacgc tcgcgagcac cagagcggcg aggcagaagt agtaggttgc
     1981 gagatgggac tcgaacagcg gatcgaggcc gggaattcct tcctcgccgc cggtgtactg
     2041 ccagaagatg cgtgtcaggc gctgcatgat ggtcgctgcg gcgagcatgg cgagagcgaa
     2101 atagggaccg cgcagccgca gcgtcggaaa gccgacgacg agactgaagg cgaccgcgac
     2161 cagcgcgccc gcgggcaatc cccaccacgg gctcagatcg agcttgctgt tgaggaaggc
     2221 ggccgtataa gcgccggcgc cgatgaagag cgcgtgaccg aaattctcgc ggccggtgag
     2281 gcccgacatg aagtcccagc tcgccgccca gatggcgtag atgacgctga ccgtgaggat
     2341 gccgctgaaa taatccgagc ggacgacaag cggtgccatg gcgagaagca cgagaaccgc
     2401 gacggcgccg accacatcct tgttgagccg gcgcatggac aaagtcaaaa cgccgcgcgc
     2461 ttgccgagaa ggcccgcggg gcggaggatg agcgtgagca gcaccgcgat cagcgatacg
     2521 atctccgtcc aggcgggcga gatgccgtag gagacgacgg tctcgctata gccgaggagc
     2581 aacgcggcga gaatgctgcc ggggatcgag cccaggccgc cgacgatgac gatggcgaag
     2641 gccttcacca tgggcagcag gtccatggtc ggctgcaccg tcagaaaagg cgcggtgagc
     2701 acgcccgcga gcgcggcgat cgccgccgag agacccatca ccaccgaata gatgcggttg
     2761 gcggggatgc ccatatattg cgccccttcg ggatcctggg agatcgccag gatggcggag
     2821 ccgagacgcg tcttctgaat gaagacatag agaagcgcga gcacggccat gccggcgccg
     2881 agcgtgagga gacgctggcc gctgatgtcg aggccggcgc aagcgcaggt ctccgtcacg
     2941 aaagccggga cgttccgcgc ctccgagccg aagagcagga acatgccctg ctcgaagatc
     3001 agcgccacgg cgagcgtgat catgagcacg gcaagcgggg agctgcgcag cggccgcacc
     3061 agcagccgct ccatcgcggc gccgaaggcg gcgacgaaac ccacggcgac caccgccgcc
     3121 agcggcaacg gccagtgcaa gagccccgtc atgacgtaag ccgcataggc gccgagcccg
     3181 tagaaggcgc catgcgacaa gttgaggatg cgggccacgc cgaagatcag cgtgaagccg
     3241 accgccagca tcgcgtagat cgcgctcgtg acggcgccgt aaacgagaat gttcagcaag
     3301 gacggctcgg gagcgaaccc cccaccctaa ccctccccct caagggcgga ggggaaaccg
     3361 atgcttgctc cgatcgaccc cctcccccct tgagggggag ggaggggagg ggggtgtgtc
     3421 gccacatcgc gacgatccgc gcggagcgct acttcgcggc ctcggcgctt tggctgaccc
     3481 agggcggcag gatcatcttg ccgctggcga gctccttcgg ccagacgatg gcgcggttcc
     3541 cgtccggctg ccattgcacg aagatcaggt tcaggaggtc cggccccggc agcacgtcgt
     3601 gatgctcgtc gaatacatag gtgccctgcg tccccatata ctttgtcttc tcgagctcgg
     3661 ggataacctt ctcggcatcg gtggtccctg cacgctcgac cgcttcgaca tagatgtgga
     3721 gcgcgtcata ggcgccgggc gcggtgtaga ccggcaagcg ctggtaacgg tccttgaagg
     3781 cgttccagaa cggcatggtc ttgggggtga gcggagcggc gaagaaggtc atgccgccgg
     3841 tctcgctgac cgacttgccg ccgatgcggg tgaagaagtc ggggtcctgg ctcttgacgt
     3901 cgataccgcc gattggcatc gggaacttgg cgtcgtacca ctgcttgacg aagacgtcgg
     3961 aattggcgtg ggagaggatg accacgagat actgcgcgcc gctctccttc accttgctga
     4021 gcagcggcga gaagtcggac atgtcgggat cgaagaggtc gagcatcgcc accttgacgc
     4081 cgcctttctc gacattttcc ttgagcaccg gcgccaggtc ctgcacccat ttcgcgttct
     4141 cgccgacaat ggcgatggtg tcgtagccga actcgccctt gacgttgctg ttgacgaagc
     4201 cggcgacttg ctcgccctgg cgcaccgcgt tgatcgggtt gacgcggaac acgtatttgt
     4261 agcggttgta gttcttgccg acgaaatcgg taatggcggg agaggctgcg ccgacgccca
     4321 tccagatggt cttggcatcg gcgatgtgcg gctcctgggc cagcgtcacg ccgctgctgt
     4381 agccgccgat gagcacatcg acatggtcat cggtggtgag cttcttgacg gcggccacgc
     4441 cttgctgcgg gtccatggtt tcgtcctcga cggcgagcgc cagcttgcgg ccaagcaccc
     4501 cgcctttggc attgatctcg tcgatgccga gctgaagggc gtccacggta tcgcggccga
     4561 cctgtagctg gatcgccgcg gtcgcgccga tgatgagggg cttctcgtcg gcgagcgccg
     4621 ccgtggcgcc gagagcgagc gttgcgagcg cgcagcataa agcggggcga tgcatgtcca
     4681 ggtcctcccg ggcgtcgttg ttgcatcgag catgcggcag caacggggca tttggcaagc
     4741 gagagcgcag gagaggtcaa gcgacgacga tcagcgcttg ggcgtgctgc tcccggcggt
     4801 cggcgtcggg gagcccgggg gattttgcgc ggcataagcc ttcgagcagg cgttggcgtc
     4861 gccgaggccg atgtcgacca gcggggcgag cgcggcgggc gggaagagca cgcccaagcc
     4921 caccgcagcg cccagccggg cgacgagggt ggcggtctcg agatgataat cgggcttggc
     4981 gaaggtgccg gtgatgtcga cgggcatgcg caagctcacg acggtggggc ccttgttgta
     5041 gggctcgaga ttgagatcga gcgtctcgtc ggcgaagttg agattgccct tgccgacgat
     5101 ttccacggcc ttggtgtcga cgagcagcgt cgacgcggtg gcgacgcccg ccttgatgtc
     5161 gaagcgcgag accatgcagt tgatctggac ggggcggtcg cccgtgacat agacgccgag
     5221 agcgcccagc acgttgatgg gcgcgagctg ctccaggagc gcgctgacct ggccgttgcc
     5281 gaggaagacg ccggcatcgc cgttcatatg
//
LOCUS       DASYNS010000531         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3565013, whole genome shotgun sequence.
ACCESSION   DASYNS010000531 DASYNS010000000
VERSION     DASYNS010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3565013"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..917
                     /locus_tag="VGP50_16395"
     CDS             300..917
                     /locus_tag="VGP50_16395"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEV8029015.1"
                     /translation="MIVEMRTYQLKPRSLAEVEKRWAEALPARMKLSPLGGFWHTDVG
                     PLNQIIHLWPYPSLAERERIRAEAVAAGIWPPRIGEFIEHMESKILMPAPFSPPLEPR
                     QLGGIYEFRSYIYKPGSIPKVIERWSAALPERLKLSPLVGAWYTEFGPLNQWVHVWAY
                     KDAGERSRVREEAVKRGVWPPPGGGDLLVKQENLLAVPASFSPLR"
     gene            complement(919..1623)
                     /locus_tag="VGP50_16400"
     CDS             complement(919..1623)
                     /locus_tag="VGP50_16400"
                     /inference="COORDINATES: protein motif:HMM:NF005458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEV8029016.1"
                     /translation="MPHAIAIRHVAFEDLGSLAAVLERRGWSVDYRDAAVADLAAPEV
                     DAADLLLVLGGPIGAYEDEAYPFLAGELRLIEQRLGAGRPVLGICLGAQLMARALGAR
                     VYPGDGKEVGWSPLQLSDAGRRSCLAPLGEGTAVLHWHGDTFDLPAGALHLAATPRYH
                     NQAFSFERHGLALQCHLEVTARGLEPWYVGHAMEIAGLTHTTVAGLRVAGQRHAPLLA
                     PRATLCFERWLAAVAP"
     gene            complement(1634..2374)
                     /locus_tag="VGP50_16405"
     CDS             complement(1634..2374)
                     /locus_tag="VGP50_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV8029017.1"
                     /translation="MAKRSNRDAQHRLQEDAVEPLFGPGWHPLGVGRDGLREQSYLRK
                     VRPQSDNQRALMAAIESHDLTLAVGPAGTGKTYLAITAAVEALEAGRVGRIVLTRPAI
                     EAGESLGFLPGDVHEKLAPYLRPLYDALNERIGGKRVRQLMTEGAIEIAPVAYMRGRT
                     LNNAFIVIDEAQNCTYGQIKMLLTRLGWHSTMVITGDPDQSDLLEGLSGFADIAERLE
                     RLPEIAVIRLTDGDIVRHPLVAGMLTVL"
BASE COUNT          505 a         1028 c         1039 g          494 t
ORIGIN      
        1 gtactcatta tcctgctttt ggtggggtga ctccgagggc ggcgctcagg ccctgagaag
       61 ctgttcgaca tcggtaagaa tgatgatcga tggcagcgtc atggctcatc gccagcgccg
      121 cgcggcgccg caatcaggca accggcgcgc gccgctatca tcgcgctgcc ctttggtctc
      181 gttctcggcg gcgcggcgct ggcgttgctc gccgcgctct atctgctcgc ccgctgagac
      241 cgctctcgcg ccgcggcgaa attcggctag actccacgct ccagacggag gaaacgccga
      301 tgatcgtgga gatgcgcacc tatcagctga aaccgcgcag cctggccgag gtggagaagc
      361 gctgggccga ggcgttgccg gcgcggatga agttatcgcc gctcggcggc ttctggcata
      421 ccgacgtggg gccgctcaac cagatcatcc atctctggcc gtatccgagc ctcgccgagc
      481 gcgagcgcat ccgcgccgaa gccgtggccg cgggcatctg gccgccgcgc atcggcgaat
      541 tcatcgagca catggagtcg aagatcctca tgccggcgcc gttctcgccg ccgctcgagc
      601 cgcgccagct cggcggcatc tacgagttcc gctcctacat ctacaagccc ggctcgatcc
      661 cgaaagtgat cgagcgctgg agcgcggcct tgcccgagcg gctcaagctc tcgccgctgg
      721 tcggcgcctg gtacaccgag ttcgggcctt tgaaccaatg ggtccatgtg tgggcctaca
      781 aggacgccgg cgaacgctcc cgcgtccgcg aagaagcagt gaagcgcggc gtctggccgc
      841 cgcccggcgg cggtgatctc ctggtgaagc aggagaacct gctggccgtg cccgcgtcct
      901 tctcgccgct tcgctgatct agggcgcgac cgcggcgagc cagcgctcga agcagagggt
      961 ggcgcgcggc gcgagcagcg gcgcgtgccg ctggcccgcg actcgcaagc ccgcaacagt
     1021 agtgtgcgtc aagcccgcga tctccatagc atggccgaca taccagggct cgaggccgcg
     1081 cgccgtgacc tcgagatggc attggagcgc aaggccgtgg cgctcgaagg agaaagcctg
     1141 gttgtgatag cggggcgtcg ccgcgagatg gagcgcgccg gccggcagat cgaaggtatc
     1201 accatgccaa tgcaacacgg ccgtcccctc gcccaacggt gcgaggcagg accggcgccc
     1261 ggcgtcgctg agctggagcg gcgaccagcc gacctccttg ccgtcgcccg gatagacccg
     1321 cgcgcccaac gcacgcgcca tgagctgcgc gccgagacag atgccgagaa ccggccggcc
     1381 cgcgccgaga cgctgctcga tcagccgcag ctcgccggcg aggaagggat aggcttcgtc
     1441 ctcataagcg ccgatcggcc cgcccagcac gaggaggaga tcggccgcgt cgacctctgg
     1501 tgccgcgagg tcggcgaccg ccgcgtcgcg gtaatcgacg ctccagccgc ggcgctccag
     1561 cacggcggcc aagctgccga gatcctcgaa ggcgacatgg cggatggcga tggcgtgcgg
     1621 catcgccgcg atcttacaag accgtgagca tgcccgcgac cagcggatgg cggacgatgt
     1681 cgccgtcagt gaggcggatc accgcgatct cgggcagccg ctcgagccgc tccgcgatgt
     1741 cggcgaagcc cgagaggccc tcgagcagat cgctttggtc gggatcgccg gtgatcacca
     1801 tggtcgagtg ccagccgagc cgggtcagca gcatcttgat ctggccgtag gtgcagttct
     1861 gcgcctcgtc gatgacgatg aaggcgttgt tgagggtccg tccccgcata taggccaccg
     1921 gcgcgatctc gatggcgcct tcggtcatga gctggcgcac gcgcttgccg ccgattcgct
     1981 cgttgagcgc gtcatagagc ggccgcaaat agggcgcgag cttctcatgg acatcgcccg
     2041 gcaggaagcc caggctctcg cccgcctcga tcgccgggcg cgtcagaacg atgcgcccga
     2101 cgcggccggc ctccagcgct tcgacggccg cggtgatggc gaggtaggtc ttgcccgtgc
     2161 cggcgggccc gacggccagc gtcaggtcat ggctctcgat cgcggccatg agcgcgcgtt
     2221 gattgtcgct ctgcggccgg accttgcgaa gataactctg ctcgcgcagg ccgtcgcggc
     2281 cgactcccaa aggatgccaa cccggcccga acaacggctc gacggcgtct tcctgcaatc
     2341 ggtgctgagc atcgcgattc gagcgcttgg ccatgccggg ttccttcgtc tgaagacaaa
     2401 aaggagccgc gccgcgagag gcggcgggcg cgaaaagcca aattgtgttg atttgcccgg
     2461 gagcagcagg tccgggcctc gaaaagagcg gcgtgagacg cacaagagcg gcgacatcga
     2521 cacaagcgac gatagccgat aaggccccgg cctgtcacga actcttgacg tgcccgcgac
     2581 gtaattctca agatgtggtg tgcggcctga tgaaccgccg tccattgcgc tcctgcgtcg
     2641 caggccgccg acggcctccc cctccaccct cgggggcgga gacggccggg gcgaggtggg
     2701 ggctcttcga cgtcacaaat tcgtcttcgg tggccgcgcg acgcaacgcg ccgctgcgag
     2761 cgggactctc gcaattgctt gcgcaatcgc tgctgccgcc gcggacgagc gcgcgacgcg
     2821 cgctcgtctc gcccacctca tcctaaatcc ttctccgccc tggaggacgg agaaggactt
     2881 gtccgtctcg cgtgacttgc actgcctatt ttatcaggcc ctcgaccttc agcgcttctt
     2941 gcactttggg gcgggcggcg acgcggtcga tgtaggtttt gaggttgggc cactggccga
     3001 gatcgatgtc cttgtacttc gaccagctcg tgatgacaaa gcaataggcg tcggccacgg
     3061 tgaagg
//
LOCUS       DASYNS010000532         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4811511, whole genome shotgun sequence.
ACCESSION   DASYNS010000532 DASYNS010000000
VERSION     DASYNS010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4811511"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VGP50_16410"
     CDS             <1..380
                     /locus_tag="VGP50_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029018.1"
                     /translation="LAEAGIQYIGDWVYDDEPTEIRTPHGPLVTLPYTVELNDIPMML
                     VQHHRADELLRRASDQFERLYREGAARAKFLSIAIHPYITGVPHRIKYLEAFLEHAAG
                     HEGVAFWTGEQILAWYRQAQSRA"
     gene            complement(397..>2141)
                     /locus_tag="VGP50_16415"
     CDS             complement(397..>2141)
                     /locus_tag="VGP50_16415"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8029019.1"
                     /translation="KPPRGFARSSGGIVFAIFCGLSAMLCSLLGYVSYQSSLNDFLRD
                     KAGEDRTAFDLADAFFSTYAAVRAPIHPTALPAPQAFRALAFERFNAARGADEGLHVS
                     LIGLPGREIATKAPDADMAAAITAMAAQANPVMRDYFYESGHGPVLRTLYPSIASQQS
                     CVDCHNGLQPGAASWKLNDVMGALVLDMPASRALADIRQHAILVGAATFLASIAIAFH
                     LMALVFRRAQSDVEQRSHALLAEAVEGLRDGISLYGSDGRVILTTAAPPPSRDPATVE
                     IAQSADGRWRQIRQSHTASGKVVRVESDITDLMEHEAALRRAKDEAEEANRAKSEFLA
                     MMSHELRTPLNAIIGFSDVMRKQTFGEIAPRYQVYAKDIHESGQHLLRVISDILDMSK
                     LDAGKAELAEGACDVAETIKRCRRLIVERAERGGVNLMAWFPPDLPGLWADEVKLRQI
                     ILNLLSNAVKFTASGGQVTVTARDLGPDEATGGLVIEVADNGIGMASKDIPVALTPFR
                     QLDNQFGRKYEGMGLGLPVCKTLIELHGGMLELKSEVDAGTIATIRFPAYRRRPPLPE
                     SPVEAPAAMVLARR"
BASE COUNT          358 a          700 c          726 g          357 t
ORIGIN      
        1 tgctggccga agccggcatc cagtatatcg gcgactgggt ctatgacgac gagccaacgg
       61 aaatccgcac cccgcatggg ccgctcgtca cgctgcccta tacggtcgag ctcaacgaca
      121 tccctatgat gctcgtccag catcaccgcg ccgacgagct gttgcgccgg gcgagcgacc
      181 agttcgagcg cctctatcgc gagggtgcgg cccgcgccaa gttcctgtcg attgcgatcc
      241 acccttacat cacgggcgtg ccgcaccgca tcaagtatct cgaggcgttc ctcgagcacg
      301 cggcgggcca tgagggtgtc gccttctgga cgggcgagca gatcctcgcc tggtatcgtc
      361 aggcccagag ccgcgcctga acgcggccct gcgtcctcaa cgtctggcga gaaccatggc
      421 ggcgggcgcc tcgacgggcg actccgggag cggcggacgc cggcgatagg ccgggaaacg
      481 gatcgtcgcg atcgtgcccg catcgacctc gctcttgagc tcgagcatgc cgccatgcag
      541 ctcgatcaag gtcttgcaga cgggcaggcc cagtcccatg ccctcgtatt tgcgtccgaa
      601 ctggttgtcg agctgccgga agggcgtcag cgcgaccgga atgtccttgg acgccatgcc
      661 gatgccgttg tcggcgacct cgatcaccag gccgccggtc gcctcatcgg gtccgagatc
      721 ccgcgcggtg acggtcacct gcccgccgga ggccgtgaac ttcaccgcgt tcgagagcag
      781 gttgagaatg atctggcgca gcttgacctc gtcggcccag agccccggca gatccggcgg
      841 gaaccacgcc atcaggttga cgccgcccct ctccgcccgc tcgacgatga gacgccggca
      901 gcgcttgatg gtctcggcga cgtcgcacgc accctcggcg agctcggcct ttccggcgtc
      961 gagcttcgac atgtcgagga tgtcgctgat gaccctgagc agatgctggc cgctctcgtg
     1021 gatgtccttg gcatagactt ggtagcgcgg cgcgatctcg ccgaaggtct gcttacgcat
     1081 gacgtccgag aagccgatga tggcgttcaa cggcgtgcgc agctcatggc tcatcatggc
     1141 aaggaattcg gacttggcgc ggttggcttc ctccgcctcg tccttggcgc gtcggagcgc
     1201 cgcctcatgc tccatgagat cggtgatatc gctttcgacg cgcaccactt tgccggaggc
     1261 cgtatgggac tggcggatct gccgccagcg gccatcggcg ctctgggcga tctcgacggt
     1321 cgccggatcg cgggacggcg gcggcgccgc ggtggtgaga atgacgcgcc cgtccgaacc
     1381 atagaggctg atgccgtcgc gcaggccctc gacggcttcg gcgaggagcg catgggagcg
     1441 ctgctccacg tcgctctgcg cgcggcggaa caccaacgcc atcaagtgaa aggcgatggc
     1501 gatgctggcg agaaatgtgg cggcgccgac caggatcgca tgctgccgga tatcggccag
     1561 cgcgcgtgaa gcgggcatgt ccagcaccaa cgcgcccatg acgtcgttga gcttccagct
     1621 cgccgcgccg ggctgcaggc cattgtggca atcgacgcag ctctgttggc tggcgatcga
     1681 cggatagagc gtacgcaaga ctggcccgtg cccggattcg tagaaatagt cccgcatcac
     1741 cgggttggct tgcgccgcca tggccgtaat ggcggcagcc atgtcggcgt ccggcgcttt
     1801 cgtcgcgatc tcgcgacccg gcaggccgat gagcgagaca tgcaggcctt catccgcgcc
     1861 gcgagcggca ttgaagcgct cgaaggcgag ggcacggaac gcctgcggcg ccgggagtgc
     1921 cgtcggatgt atcggcgccc tgaccgcggc gtaggtcgaa aagaacgcgt cggccaggtc
     1981 aaaggcggtg cggtcttccc ccgctttgtc gcgtagaaag tcgttgagcg aggactggta
     2041 ggagacgtag ccgaggagac tgcagagcat ggcggacagg ccgcagaaaa tggcgaacac
     2101 gattccgccg ctcgaccgcg cgaagccgcg cggcggcttc a
//
LOCUS       DASYNS010000533         6744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4657025, whole genome shotgun sequence.
ACCESSION   DASYNS010000533 DASYNS010000000
VERSION     DASYNS010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4657025"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..1122)
                     /locus_tag="VGP50_16420"
     CDS             complement(316..1122)
                     /locus_tag="VGP50_16420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8029020.1"
                     /translation="MPTVRANGITMNYDRQGSGEPLLLIPYLAADYACYAFQVADYAK
                     HFTCISVDPRGAGASDAPGGTYSTELFADDIAAFLAEIGIAKAHVSGLSLGAATGMWL
                     AAKYPGKVKTLSLHSGWSKSDPFLKAVVEGWQTMAKGLGNVTEMVIQAIFPWCLTPEL
                     YAAKPDYIESLAAFVRGRPQQPLDAFLQQSSAVIAHDASAQLGRIAAPTQITFGRHDM
                     VTSTRFAKAMQSAIRGSELIVFEGCAHAPIYEDVAAFNQRTLAFLKEHTG"
     gene            complement(1311..2126)
                     /locus_tag="VGP50_16425"
     CDS             complement(1311..2126)
                     /locus_tag="VGP50_16425"
                     /inference="COORDINATES: protein motif:HMM:NF006108.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEV8029021.1"
                     /translation="MTVRIEKDGAVWTIIHDRPEARNAMDPASADALTAAFLAFDADK
                     TASVAVFYGAGGAFCAGWDLKYVSTLDDKYPLGELDIPVAGPRGNGAEIPRGPLGPSR
                     LELDKPVIAAIEGPAVAGGMELGLWCDFRVMAKGAYFGVYCRRWGVPLIDGGTVRLPR
                     IVGQGRALEIILTGRKVPAEECLSIGLCEYVTPEGGARAKAEALAREIARFPQVCARA
                     DRRSAIRSHGLATRDALIQEWYNGREALIEDGVAGASRFKDGLGRHGDFDKIR"
     gene            complement(2287..3168)
                     /locus_tag="VGP50_16430"
     CDS             complement(2287..3168)
                     /locus_tag="VGP50_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV8029022.1"
                     /translation="MRLVAFEEAGHPRLGVVERDGVVDLERAAPELPRDLLGLIRAGD
                     AALAAAAGAAAKAGAASRRPLAGLRFRPPLENAGKILCLGLNYVDHAAEGGHAKPDYP
                     SVFMRCNTSLVAHGEAILRPRASSQLDYEAELVAVIGRTARHVAVRDALSHIAGYSCF
                     NDASLRDYQRKTSQWTIGKNFDRTGAFGPWLVTADALPPGAAGLAIRSRLNGRVMQDA
                     NTGDMISPVAETVALLSECLTLEPGDLLVMGTPAGVGYARKPPVWMKDGDTVEIEIEG
                     IGILANPIRDEPASAPR"
     gene            3292..4539
                     /locus_tag="VGP50_16435"
     CDS             3292..4539
                     /locus_tag="VGP50_16435"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV8029023.1"
                     /translation="MGGLRGKLALWGAVAFLSIAPALAAEPIRIGFGMALTGGLAANG
                     KSALVAMQIWRDDVNAAGGLLGRPVDLVYYDDQSNPSTVPQIYTKLLDVDKVDLVVGG
                     YATNMIAPAMPVVMQRHMTLIGLLGLAVNSAFGYPRYFAMIPTGPDPKIGFTKGFFDL
                     AMAQNPRPKTIAIVAADAEFARNAADGARQNAKAAGLEIVYDRSYPPSTVDYTPIVRA
                     IQAANPDIVYVGSYPPDTAGMVRAINEVGLDVKLCCGGMVGLQSAALKTQLGAQLNGI
                     VNYDFWLPAPTMVFPGVLELVKRYQEIAPKEGVDPLGYYMPPFAYAYLQVLGQAVEGT
                     KSLDQGKLAEYIHSHSFKTVVGEIKFADNGEWAEGRVLQIQFQGIKGNDLEQFKNPAT
                     QPILAPAPDASGSIIYPRAEARR"
     gene            4543..5535
                     /locus_tag="VGP50_16440"
     CDS             4543..5535
                     /locus_tag="VGP50_16440"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8029024.1"
                     /translation="MKAAACLALALCACAAVPAARAEVKTLDVIVFAGGFNWPIWAAT
                     QQGYFAQSGVAVHLTPTPGSEFQLKNLIEGKFDIAMTAIDNVVAYQEGQGEAQVSVAP
                     DLFVFMGGDNGFLRLVAVPEVKSYADLKGKELTVDARTTGYAFVLEKMLEKGGLGPGD
                     YQLVKAGGVLARFDDLMQKRHAATLLLSPFEVPAEAKGFTRLGNAMDVLGHYQGLVGA
                     ARRDWATAHRPELVGYIRGYVEGLAWLHDAKNKDAAVALLRANLPNMAPELAAKAYDI
                     LLDRKDGFAPRAEIDMAGVRSVLALRSEYGEPRKTLDDPARYVDLSYYKEALAP"
     gene            complement(5545..>6744)
                     /locus_tag="VGP50_16445"
     CDS             complement(5545..>6744)
                     /locus_tag="VGP50_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008492552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8029025.1"
                     /translation="LTPQQRNAVLASFLGWTLDAFDFFLLVFVIKDIAAEFGTDVKSV
                     TFAIFLTLAMRPLGAFVFGLLADRYGRRPTLMVDIILFSVLELASAFSPSLTVLIVLR
                     ALYGIAMGGEWGIGASLAMETIPPGARGIVSGMLQEGYAAGYLLAAIVYYFLFPLIGW
                     RGMFVVGVLPALLVLYIRIHVKESPVWEQGAQRRENPLRAIRQHWQLFLYVILLMTAF
                     NFFSHGTQDLYPTFLQVQHKLSSHAVGAIAIVYNIGAILGGVLFGAWSERLGRRNAII
                     IAALLALPIIPLWAFSETVWQLTLGAFLLQFMVQGAWGIVPVHLNELSPEAARGTFPG
                     FTYQLGNLLAAGNATIQASIAESHGGNYGLALALVAAIVALAVAVLAWLGRERKGVAF
                     GATSAAE"
BASE COUNT         1174 a         2172 c         2341 g         1057 t
ORIGIN      
        1 aggccgccgc aggcgaggat tccgataacg atcgcggtgc gacggagatc gcgacggcga
       61 gcaagagcga cagggatgga atgcatggtc ttctccttgg gtgcgggcgc gacgatgcgg
      121 cccttcacgc cctcatgcgt atgacatggg agcggaggcg gttgaggttt agtccggaga
      181 aaacgaacac accgttcatc gccgctgtcc ggtgacaacg caccgcgcgc tctgaaggtc
      241 cctcggccga aaagaagaca ctggttgcca gtttattcgc cgaaggggat cggcgattcg
      301 attcaggcgc cgccgctatc ccgtgtgctc cttgaggaag gccagcgtcc gctgattgaa
      361 cgctgccaca tcctcgtaga tcggcgcatg cgcgcagccc tcgaagacga taagctcgct
      421 gccgcggatg gcgctttgca tcgccttggc aaagcgtgtc gaggtcacca tgtcgtggcg
      481 accgaaggtg atctgcgtcg gcgcggcgat ccggccgagc tgcgccgacg catcatgcgc
      541 gatgacggcg ctcgattgct gcaggaaggc gtcgagcggt tgctgcggcc gtccgcgcac
      601 gaaagcggcg agggattcga tgtagtcggg cttcgctgcg taaagctcgg gcgtcaggca
      661 ccagggaaag atggcctgga tcaccatctc ggtgacgttg ccgaggcctt tcgccatggt
      721 ctgccagccc tcgaccacgg ccttgaggaa aggatcgctc ttgctccagc cgctgtggag
      781 cgacagcgtc ttcaccttgc cgggatattt cgccgcgagc cacatcccgg tcgccgcgcc
      841 cagcgacagg ccggacacat gggccttggc aatgccgatc tccgcgagaa acgcggcgat
      901 gtcgtcggcg aagagctcgg tcgaataagt gccgcccggc gcgtcgctgg cgcccgcgcc
      961 gcgcggatcg accgagatgc aggtgaagtg cttggcgtaa tccgccacct ggaacgcata
     1021 gcaggcgtag tccgcagcca gatagggaat gagcagcaag ggctcgccgc tcccttgccg
     1081 gtcgtagttc atggtgatgc cgttggctct gacggtcggc atgagcgtct cctcccttgc
     1141 gttttctcgg ttgtgtcgtg ggcgctctcg ctgctgtcgc cgatgggcgc gccacgatcg
     1201 ggatggtccg ccgcgtcgct cacggcgtca aggacaaagc cgcgccggcg cgatgtcggg
     1261 cgacgcgcgc ggtgtgctct ttcgcggtaa ccgacgatcg caagcgacgc tcagcggatc
     1321 ttgtcgaaat cgccgtgacg gccgaggccg tccttgaagc ggcttgcgcc ggcgacgccg
     1381 tcctcgatga gcgcttcgcg gccgttgtac cattcctgaa tcagcgcatc gcgggtggcg
     1441 aggccgtggc tgcggatggc ggagcggcgg tcggcgcggg cgcagacctg cgggaagcgc
     1501 gcgatctcgc gcgccagcgc ctcggccttc gcgcgcgcgc cgccttcggg cgtcacatat
     1561 tcgcagaggc cgatgctcaa gcattcctcg gccggtactt tccgcccggt gaggatgatc
     1621 tcgagcgcgc gtccctggcc gacgatgcgc ggcaggcgca ccgtgccgcc gtcgatcagc
     1681 ggcacgcccc agcgccggca atagacgccg aaatatgcgc ccttggccat gacgcggaaa
     1741 tcgcaccaga gcccgagctc catgccgccg gccacggcag ggccctcgat cgcggcgatg
     1801 acgggcttgt cgagctcgag ccgagacggg ccgaggggac cccgcggaat ctcggcgccg
     1861 ttgccgcgcg ggcccgccac ggggatgtcg agctcgccca gcggatactt gtcgtcgagc
     1921 gtgctgacat atttgagatc ccagccggcg cagaacgcgc cgcccgcgcc atagaagacc
     1981 gcgaccgatg ccgtcttgtc ggcgtcgaag gcaagaaaag cggcagtgag cgcatcggcg
     2041 ctggcggggt ccatggcatt tcgcgcctcg ggccggtcgt gaatgatggt ccagaccgcg
     2101 ccgtctttct cgattctgac tgtcatcgcg tcccctcgcc gttgatcgat gcgagacgct
     2161 tcatagcgcg gcgcgcgctc ccgtcgatac gttggcaaga tcggcggtgt ctcgagatcc
     2221 gggcgatccc ggcgcgcggc cctttctcgc ggcgatgagc gacgtcctcg ccgcgattgt
     2281 attccgtcag cgcggcgcgc tcgccggctc gtcgcggatc gggttggcga ggatgccgat
     2341 gccttcgatc tcgatctcga ccgtgtcgcc gtccttcatc cagaccggcg gcttgcgcgc
     2401 atagccgacg ccggcgggcg tgcccatcac caagaggtcg cctggctcga gcgtcaggca
     2461 ctccgagagc agcgccaccg tctcggcgac gggggaaatc atgtcgccgg tgttggcgtc
     2521 ctgcatgacg cggccgttga ggcgcgagcg gatggcgagc ccggcggcac ccggcggcag
     2581 cgcatccgcg gtcacgagcc aggggccgaa agcgccggtg cggtcgaaat tcttgccgat
     2641 cgtccattgc gaggtcttgc gctgatagtc gcgcagcgac gcgtcgttga agcaggaata
     2701 gccggcgata tgggaaagcg cgtcgcgcac cgcgacgtgg cgcgcggtgc ggccgatcac
     2761 cgccacgagc tcggcctcgt agtcgagctg ggaggaggcg cgaggccgca ggatggcctc
     2821 gccatgcgcg accagcgacg tgttgcagcg catgaagacg ctcggataat cgggcttggc
     2881 gtgaccgcct tcggccgcgt ggtcgacgta gttgaggccg aggcagagga tctttccggc
     2941 attctcgagg ggcggacgga agcgcaggcc ggccagcgga cggcgcgacg cggcgccggc
     3001 cttggctgca gcgccggcgg ctgccgccaa agccgcgtcg ccggcgcgga tcaggccgag
     3061 gagatcgcgc ggcagctcgg gtgcggcgcg ctcgagatcg acgacgccgt cgcgctcgac
     3121 cacgcctagg cgcgggtgac ccgcttcctc gaaggcgacc agccgcatgt gtcctcccct
     3181 tggattgcgc aaaatccgtt ttgcggacgc cgccgacgcc gctggcggga ccgggcgtat
     3241 tgagcatatg ataaagccaa catcaatgaa aacgggggag ggcgtggcgt gatggggggc
     3301 ctgagaggca agctcgcctt gtggggcgcg gtggcgtttc tttcgatcgc gccggcgctg
     3361 gcggccgagc cgatccgcat cggcttcggc atggcgctga ccggcggact cgccgccaac
     3421 ggcaaatcgg cgctcgtcgc gatgcagatc tggcgcgacg acgtcaacgc cgcgggcggc
     3481 ctcctcgggc gccccgtcga tctcgtctat tacgacgacc agagcaatcc ctcgaccgtg
     3541 ccgcagatct acaccaagct cctcgacgtc gacaaagtcg acctcgtcgt cggcggctat
     3601 gccaccaaca tgatcgcgcc ggcgatgcct gtggtgatgc agcgccacat gaccttgatc
     3661 ggccttctcg gcctcgccgt gaacagcgcg ttcggctacc cccgctattt cgcgatgatc
     3721 ccgaccgggc ccgacccgaa aatcggcttc acgaagggct tcttcgatct ggcgatggcg
     3781 cagaacccgc ggcccaagac aattgccatc gtcgccgccg acgccgaatt cgcccgcaac
     3841 gccgcggacg gcgcgcggca gaatgccaag gccgccgggc tcgagatcgt ctacgaccgc
     3901 agctatccgc cctccaccgt cgactacacg ccgatcgtcc gcgccatcca ggcggccaat
     3961 cccgacatcg tctatgtcgg ctcttatccg ccggataccg cgggcatggt gcgcgcgatc
     4021 aacgaggtcg gcctcgacgt caagctgtgc tgcggcggga tggtcgggct gcaatcggcg
     4081 gcgctcaaga cgcagctcgg cgcgcagctc aacggcatcg tcaactacga tttctggctg
     4141 cccgcgccga ccatggtgtt tcccggcgtg ctggagctgg tgaagagata tcaggagatc
     4201 gcgcccaagg aaggcgtcga tccgctcggc tattacatgc cgcctttcgc ctatgcctat
     4261 ctgcaggtgc tgggccaagc cgtcgaaggg acgaagagcc tcgaccaggg caagctcgcc
     4321 gaatacatcc acagccacag cttcaagacc gtcgtcggcg agatcaaatt cgccgacaac
     4381 ggcgagtggg cggaggggcg tgtcctccag atccagtttc agggcatcaa aggcaatgac
     4441 ctcgagcagt tcaaaaaccc ggcgacccag ccgatcctgg cgcccgctcc cgacgcctcg
     4501 ggcagcatca tctatcctcg cgcggaagcg cggaggtagg cgatgaaggc cgcggcctgc
     4561 ctcgcgctcg ccctctgcgc ctgcgccgcc gtgccggcgg cccgggccga ggtcaagacg
     4621 ctcgacgtca tcgtcttcgc cggcggcttc aactggccga tctgggcggc gacgcagcag
     4681 ggttatttcg cgcagagcgg ggtcgccgtc cacctcacgc cgacgcccgg ctcggagttc
     4741 cagctcaaga acctgatcga aggcaaattc gacatcgcca tgacggcgat cgacaacgtc
     4801 gtcgcctatc aggaagggca gggcgaggcg caagtcagcg tcgcacccga tctcttcgtc
     4861 ttcatgggcg gcgacaacgg cttcttgcgc ctggtcgcgg tgcccgaggt caagagctac
     4921 gccgacctca agggcaagga gctgaccgtc gacgcgcgca cgacgggcta tgccttcgtg
     4981 ctcgagaaga tgctcgagaa aggcggcctc gggcccggcg actaccagct ggtcaaggcg
     5041 gggggcgtgc tcgcgcgctt cgacgacctg atgcagaagc gccatgccgc gacgctgctg
     5101 ctctcgccct tcgaggtgcc ggccgaggcc aaggggttca cgcgcctcgg caatgccatg
     5161 gacgtgctcg gccattacca agggctggtc ggcgccgctc gccgcgactg ggcgacggcg
     5221 catcgccctg agctcgtcgg ctatatccgc ggctatgtcg agggcctcgc ctggctccac
     5281 gacgcaaaga acaaggacgc ggcggtcgcg ctgctgcgcg ccaacctgcc gaacatggcg
     5341 cccgagcttg cggccaaagc ctatgacatc ctgctcgatc gcaaggatgg gttcgcgccc
     5401 agggccgaga tcgacatggc gggcgtgcgg agcgtgctcg cgctgcgctc ggaatatggc
     5461 gagccgagga agacgctcga cgacccggcg agatacgtcg atctcagcta ttacaaagaa
     5521 gcgctcgcgc catagcgcgg ggcatcattc cgccgcggag gtcgcgccga aggcgacgcc
     5581 tttgcgctcg cggccgagcc aggcgaggac ggcgacggcg agcgcgacga tggcggcgac
     5641 gagggcgagc gccaagccgt aattgccgcc atggctctcg gcgatgctcg cctggatggt
     5701 ggcattgcct gccgcaagaa gattgccgag ctgataggtg aagcccggga aggtgccgcg
     5761 cgcggcctcg ggggagagct cgttcagatg gacggggacg atgccccagg cgccctgaac
     5821 catgaactgc aggaggaagg cgccgagtgt cagctgccag acggtctccg agaaggccca
     5881 gagcggaatg atcggcaagg cgaggagagc ggcgatgatg atcgcgttgc ggcgaccgag
     5941 gcgttccgac cacgcgccga agaggacgcc gcccaggatg gcgccgatgt tgtagacgat
     6001 ggcgatggcg ccgacggcat ggctcgaaag cttgtgctgg acctggagga aggtcgggta
     6061 gagatcctgc gtgccgtggc tgaagaagtt gaacgcggtc atcagcagga tgacatagag
     6121 gaagagctgc cagtgctggc ggatggcgcg gagcgggttc tcgcggcgct gcgcgccttg
     6181 ttcccacacc ggcgattcct tcacatggat gcggatatag agcacgagca gcgctggcaa
     6241 gacgccaacg acgaacatgc cgcgccagcc gatcagcggg aacaggaaat agtagacgat
     6301 ggcggccagc agatagcccg cggcatagcc ttcctggagc atgccggaga cgatgccgcg
     6361 ggcgccgggc gggatcgtct ccatcgccag agaggcgccg atgccccatt ctccgcccat
     6421 ggcgatgccg tacagcgcgc gcagcacgat gagcacggtg agcgacggcg agaaggcgga
     6481 ggcgagctct agcaccgaaa agaggatgat gtcgaccatc aaggtcgggc gccgcccata
     6541 gcggtcggcg agcagcccga aaacgaaggc gcccagcggc cgcatcgcca gcgtcaggaa
     6601 aatggcgaag gtcacgctct tgacgtcggt cccgaactcg gcagcgatgt ccttgatgac
     6661 gaagacgagg agaaagaagt cgaaggcatc gagcgtccag ccgaggaagc tcgcgagaac
     6721 ggcgttgcgc tgttgcggcg tgag
//
LOCUS       DASYNS010000534         5054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4972401, whole genome shotgun sequence.
ACCESSION   DASYNS010000534 DASYNS010000000
VERSION     DASYNS010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4972401"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VGP50_16450"
     CDS             complement(<1..659)
                     /locus_tag="VGP50_16450"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEV8029026.1"
                     /translation="MDRPKDKPPAEPARAALPARSPSAAEVMAYLKQNPDFLLEHPDL
                     LEVLTPPTRQSGERVLDMQQFMVQHHRSEIARLKNQHRALVTTSRANLASQARVHAAV
                     LALLGASSFEQLIQTVTTDLAVLLDADVVTIAVESAGAQRPRLSHQGVQILEPGTVDS
                     VLGADRDVLMQGDAEGDPMLFGDGAGLVRSAALLRLSVSAGAPAGLLCIGMRRPGKFH
                     GG"
     gene            complement(665..1345)
                     /gene="fsa"
                     /locus_tag="VGP50_16455"
     CDS             complement(665..1345)
                     /gene="fsa"
                     /locus_tag="VGP50_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012975060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV8029027.1"
                     /translation="MKFFVDTADIAEIRDLAATGLVDGVTTNPSLVAKTGRNFLEVVR
                     EICSIVPGPVSAEVTATDHGTMLAEGRKLAKLARNVAVKVPLTPDGLKTCRALANEGT
                     MVNVTLCFSAAQALLAAKAGASFISPFVGRLDDVGSDGMALIGDIVHIYGNYPALKTE
                     VLVASIRHPIHVIAAAKLGAHVGTLPPNVLRQMFAHPLTDKGLATFLADWAKTGQSIL
                     GTSKAAAE"
     gene            1418..3658
                     /locus_tag="VGP50_16460"
     CDS             1418..3658
                     /locus_tag="VGP50_16460"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV8029028.1"
                     /translation="MPDRHASSRATGDGAEATHAAIRLAVLLPLPLAGVYDYLASSAL
                     ALEPGDFVRVPLGRRRVEGVVWGEATGDVATSKLKAVVDRLAAPPLSEELRRLVDWVA
                     SYTLAPPGAVLRMAMSVTEALEPARALTGYAPSEAGRAALAAKDRPLTAARRRVLESA
                     AEGPPAPAADLARRAGCGVGVVRALAKEGLLEALDIPARPPPAAPDWRRPGPSLSSAQ
                     QLAADDLAAKVNAGGFGVTVLDGVTGSGKTEVYFAAITAALMRRKQVLVLLPEIALSA
                     QWLARFAERFGSAPALWHSDLGRAERRDTWRAVAEGRVSVLVGARSSLFLPFPDLGLI
                     VVDEEHDASFKQEEGVVYHARDMAVVRASLAKLPIVLVSATPSLETTVNVEQRRYDRL
                     HLPARHGTAGLPEIRVIDLRRQRPERQRFLAPPLVAALAETLEQGEQSMLFLNRRGYA
                     PLTLCRACGFRLQCPNCTAWLVEHRFHGRLQCHHCGHTEPLPPLCPSCGAAASFAACG
                     PGVERLAEETASRFPKARIAVMASDTLIGPRAAAALVESVEKHEIDVLIGTQVVAKGH
                     HFPLLTLVGVVDADLGLSGGDLRAGERSFQLLQQVAGRAGRAERPGVVYLQTYAPEHP
                     VMRALVSDDRDHFLAMEAEERREQGMPPFGRLAALIISGPDDESADLAARAFARAAPH
                     MPDVEVLGPAPAPLAVLRGRHRRRFLLKTKRNVNIQAVLRQWLGKVKLPGTIRVHPDI
                     DPYSFL"
     gene            complement(3659..3823)
                     /locus_tag="VGP50_16465"
     CDS             complement(3659..3823)
                     /locus_tag="VGP50_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029029.1"
                     /translation="MIANAGFRFLPRPPDHRAAAAGHDLIPGLSEPQQNLTRRHMGAH
                     QVEREFPLSP"
     gene            3813..4679
                     /locus_tag="VGP50_16470"
     CDS             3813..4679
                     /locus_tag="VGP50_16470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV8029030.1"
                     /translation="MAIIFAFLSAACFGLALVTGRVGLRLADPRTGAAISIPSATILF
                     IIAAPFAPGIAGLNLHAALLFAAVGLVFPAVVTILTFRSNELLGPTITSAVSGTAPLF
                     ALLGAALLLGERIPPQAALSAAGVIAGVALLSWKPGATLRMVLFDWRLMVPIAGAMVR
                     GFSQVGAKEALHLWPSPFAAGFIGYVVSSAVVIVARQYQRSARSRLTAKGIAWFAATG
                     VLNGGAVLLLYAALAIAPVWKVTPIVASYPLITGVVSAVFLRDERLSLQVVTGAGMTV
                     AAVVFLVSLGGG"
BASE COUNT          683 a         1751 c         1721 g          899 t
ORIGIN      
        1 ccgccatgga acttgccggg ccggcgcatg ccgatgcaga ggaggcccgc cggcgcgccc
       61 gcgctcaccg agagccgcag cagggcggcc gagcgcacga ggcccgcgcc gtcgccgaac
      121 agcatcggat cgccttcggc atcgccttgc atcagcacgt cgcgatcggc gccgagcacg
      181 ctgtcgacgg tgccgggctc gaggatctgc acgccttgat ggctcaagcg cgggcgctgc
      241 gctcccgccg attcgaccgc gatcgtcacg acgtcggcgt cgagcagcac ggcgagatcg
      301 gtggtgacgg tctggatgag ctgctcgaag ctgctcgccc ccagcagcgc cagcactgcc
      361 gcatggacgc gcgcctggct tgcgagattg gcgcggctcg tcgtcaccag cgcgcgatgc
      421 tggttcttga gccgggcgat ctccgagcga tggtgctgaa ccatgaattg ctgcatgtcg
      481 agcacgcgct cgccgctttg ccgcgtgggc ggcgtcagca cttcgaggag atcgggatgc
      541 tccagcagga aatcgggatt ctgcttcaga taagccatga cctcggcggc gctcgggctg
      601 cgcgccggga gcgcggcgcg ggccggctcg gcggggggct tgtccttcgg gcgatccaag
      661 cgggctattc cgccgcggcc ttgctcgtgc cgaggatcga ctggccggtc ttcgcccaat
      721 ccgcgaggaa cgtggcgagg cctttgtcgg tcagcggatg agcgaacatc tggcgcagca
      781 cgttgggcgg cagggtgccg acatgggcgc ccagtttcgc cgccgcgatc acatggatgg
      841 gatggcgaat cgaggcgacg agcacctcgg tcttgagcgc gggataattg ccatagatgt
      901 ggacgatgtc gccgatgagc gccatgccgt cgctgccgac gtcgtcgagc cgcccgacga
      961 agggcgagat gaagctggcc cccgctttcg ccgcgagcag cgcctgcgcc gccgagaagc
     1021 agagcgtcac gttgaccatc gtgccttcgt tggcgagtgc gcggcaggtc ttgaggccgt
     1081 cgggcgtcag cggcaccttg accgcgacat tgcgcgcgag cttcgcgagc ttgcgccctt
     1141 cggccagcat cgttccatga tccgtcgccg tcacttcggc gctcaccggt cccggcacga
     1201 tactgcagat ctcgcgcacg acctcgagga agttgcgccc ggtcttcgcc accagcgacg
     1261 gattcgtggt gacgccgtcg acgagcccgg tcgccgcgag gtcgcggatc tccgcgatgt
     1321 cggcagtgtc gacgaaaaac ttcatccgcg ccgatctccc ccggcatgtt tccgcgctgc
     1381 tggaacgcgc gctgctttcg aactaagagt gtagccgatg cccgaccgcc acgccagttc
     1441 tcgcgcgacc ggtgatggtg cagaggcaac gcatgcggcg atccgtctcg ccgtgttgct
     1501 gccgctgccg ctcgccggcg tttacgacta tttggcatcg agcgcgctcg cgctcgagcc
     1561 gggcgatttc gtgcgcgtgc cgctgggacg ccgccgcgtc gaaggcgtgg tgtggggtga
     1621 agcgaccggc gatgtcgcga cgtcgaagct gaaggcggtc gtcgatcggt tggccgcgcc
     1681 gccgctttcc gaagagctgc gtcgcctcgt cgattgggtc gcgtcctaca ccttggcgcc
     1741 gcccggcgcc gtgctgcgca tggcgatgag cgtcacggaa gcgctcgagc cggcgcgcgc
     1801 cctcaccggc tatgccccga gcgaagcggg ccgcgccgcg cttgcggcga aggatcgtcc
     1861 gctcacggcg gcgcgccggc gcgttctcga gagcgcggcc gaaggaccac cggcgcccgc
     1921 cgcggatctg gcgcggcgcg ccggctgcgg cgtcggcgtc gttcgcgcgc tcgcgaaaga
     1981 aggtctgctc gaggcgctcg acataccggc gcgccccccg cccgccgcac cggactggcg
     2041 acgtccgggt cccagcctct cctcggcgca gcaactcgcg gcggatgatc tcgccgccaa
     2101 ggtgaatgcc ggcggcttcg gcgtcaccgt cctcgacggc gttaccggct cgggcaagac
     2161 cgaggtttat ttcgccgcga tcacggccgc gctcatgcgc cgcaagcaag tgctcgtgct
     2221 cttgccggag atcgcgctca gcgcccaatg gctcgcgcgc tttgccgagc gcttcggctc
     2281 ggctcccgcg ctctggcatt ccgatctcgg ccgtgccgag cgccgcgata cctggcgcgc
     2341 cgtcgccgaa ggccgcgtct ccgttctcgt cggcgcgcgc tcgtcgctct tcctgccgtt
     2401 tcccgatctc ggcctcatcg tcgtcgacga ggagcatgac gcttccttca agcaggaaga
     2461 gggtgtcgtc tatcacgcgc gcgacatggc ggtggtgcgc gcatcgctgg cgaaactgcc
     2521 gatcgtgctc gtgtcggcga cgccgtcgct cgagaccacc gtcaatgtcg agcagcgccg
     2581 ctacgatcgc ctccatctcc cggcgcgcca cggcaccgcg ggcctgccgg agatccgcgt
     2641 catcgacctg cgccgccagc ggccggagcg tcagcgcttc ctcgcgccgc ctttggtcgc
     2701 cgcgctcgcc gagacgctgg agcagggcga acagtcgatg ctgttcctga accgccgcgg
     2761 ctatgcgccg ctcacgctct gccgcgcttg cggctttcgc ctgcaatgcc cgaactgcac
     2821 ggcttggctc gtcgagcacc gcttccatgg ccggctccaa tgccatcatt gcggccatac
     2881 cgagccgctg ccgccgctct gtcccagttg cggtgctgcc gcgagtttcg ctgcttgcgg
     2941 tcccggcgtc gagcgcctcg ccgaggagac ggcgtcgcgt ttccccaagg cgcgaatcgc
     3001 cgtgatggcg agcgacacgc tgatcggccc gcgcgccgct gcggcgctgg tggagagcgt
     3061 cgagaagcac gaaatcgacg tgctcatcgg cacgcaagtg gtggcgaagg gtcatcactt
     3121 cccgctgctg acgctggtcg gcgtggtcga cgccgatctc ggcctttccg gcggcgatct
     3181 ccgcgccggc gagcggagct tccagctgct tcagcaagtg gcgggccgtg ccggccgcgc
     3241 cgagcgtccg ggcgtcgtct atctccagac ctatgcgccc gagcatccgg tgatgcgcgc
     3301 cctcgtctcc gacgaccgcg accacttcct ggcgatggaa gccgaggagc ggcgcgagca
     3361 gggcatgccg cccttcggcc gcctcgccgc gctcatcatc agcggtcccg acgacgagag
     3421 cgccgatctc gctgcgcgcg ccttcgcccg tgccgcgccg cacatgcccg atgtcgaagt
     3481 gctgggcccg gccccagcgc cgctcgccgt tctgcgcggc cgccaccgcc gtcgcttttt
     3541 gttgaagacc aagcgcaacg tcaacatcca ggcggtgctc cgccaatggc tcggcaaggt
     3601 aaagctcccc ggcaccatcc gcgtccaccc cgacatcgac ccctacagct tcctgtgatc
     3661 acggcgagag cggaaattcc ctctcgacct gatgggctcc catatggcgg cgtgttagat
     3721 tctgctgcgg ctcgctgaga cccgggatca gatcgtggcc ggcagcggcc gcgcggtggt
     3781 cgggtggacg tggcaggaaa cggaagccgg cattggcgat cattttcgcg ttcctgagtg
     3841 cggcatgctt cggtcttgct ctcgtcaccg ggcgtgtcgg tctccgcttg gccgaccccc
     3901 gcaccggcgc ggcgatcagc atcccgagcg cgacgattct cttcatcatc gccgcgccgt
     3961 ttgcgcccgg catcgccggc ctcaaccttc acgcggcgtt gctgttcgcc gcggtcggcc
     4021 tggtctttcc tgccgtcgtc accattctga catttcgatc caacgagctt ctcggcccga
     4081 cgatcaccag cgccgtatcg ggaaccgctc cgctttttgc gctgctcggg gcggcgctgc
     4141 tgctcgggga gcggatacct ccgcaggccg cgctttccgc ggccggcgtg atcgcaggcg
     4201 tcgctctcct gtcatggaag ccgggagcga ctctgcgcat ggttcttttc gattggcggc
     4261 tgatggtgcc gatcgccggg gcgatggtga gaggattttc ccaagtcggc gccaaggagg
     4321 cgttgcatct ctggccgagc ccgttcgccg ccggctttat cggatatgtc gtgtcctctg
     4381 ctgtcgtgat cgtcgctcgt cagtatcagc gctcggcgcg gtcgaggctg accgcaaagg
     4441 ggatcgcctg gtttgccgcg accggcgtgc tgaacggcgg ggcggtccta ctgctatatg
     4501 cggccttggc catcgctccg gtctggaagg tgacgccgat cgtcgcctcc tacccgctga
     4561 ttacgggcgt cgtcagcgcc gtctttttgc gtgacgagag gctgtcgctc caggtggtga
     4621 cgggggcggg catgacagtg gcggccgtcg tctttttggt cagtctgggt ggcgggtgat
     4681 cgcgttttgc gcggacgaat gcaagcggac atgccgccct cctgccgagg ggtctctcgc
     4741 gcgatggtgt cgaacccacg gggccgctcg gtcgattttc ccggaggaaa tccaaggaaa
     4801 tcgtggcttg accgagggtc gtcccccgtg ttgcgccgca cgcgaccctg tgctagaacg
     4861 cgccgcgctt tccggggcgc cccctcggcg cagcgggttt tccgcgggaa agcgcatatc
     4921 acatcccacg gcgacctaat tctcgtccag gggcaacgag gaccgatccc gacgtggcag
     4981 ccgaagcgaa aggcgtatcc ggtctcgctg tgcgttacgc gctggcgctg ttcgatctgg
     5041 ccgacgagcg caag
//
LOCUS       DASYNS010000535         7297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3256632, whole genome shotgun sequence.
ACCESSION   DASYNS010000535 DASYNS010000000
VERSION     DASYNS010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3256632"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VGP50_16475"
     CDS             <1..810
                     /locus_tag="VGP50_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV8029031.1"
                     /translation="VQAISPSPPQYYYWAAPELGREAARLVNNRIAEIVATHPDRFVG
                     LGTVPLQAPDLAVAELERMVKELGLRGVEICTNVNGAELSEQRFRPFFAKAEELGILV
                     FMHPNGFTEGRRLADHYFINVIGNPLDSTIAVSHLIFGGVLDAHPRLKICVAHGGGFL
                     PAYSGRMDHAHGARSDCRREITKKPTRHLAKLYFDTIVFTHDQLEYLVQRWGSDHILL
                     GSDYPFDMAEPDPVGFVSGAALSGDDKAAILGRNAAKLLKIRIPAAPRRKS"
     gene            875..1519
                     /gene="maiA"
                     /locus_tag="VGP50_16480"
     CDS             875..1519
                     /gene="maiA"
                     /locus_tag="VGP50_16480"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEV8029032.1"
                     /translation="MKLYGFWRSQATFRVRIALNLKGLKREDVFINLDHGDQHSAAYK
                     ALNPQAVLPTLFDGDAKLFQSFAILEYLEERYPKPPILPADPLARAWVRGFALINVAD
                     SHPLIVPRIRNYLTDVLKVDEAAKTAWIQHWLAAGLEAMERLLAEKKPAGRFCHGDSP
                     TVADICLMTQVTPAQIFKCTLDPYPLTMRIYETCMSIPAFADAHPRKQPDFAPH"
     gene            1536..2366
                     /locus_tag="VGP50_16485"
     CDS             1536..2366
                     /locus_tag="VGP50_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEV8029033.1"
                     /translation="MTLDIRPIAGALGAEIHGIDLDVGLDAQTIAAIRQALLDHLVIF
                     FREQRLPPDRFLAFARRFGRPVEYPFVKGIEGHPEIIAVTKREDERVNFGGIWHSDTT
                     YLAEPPMGTMLIAREVPPYGGDTLFANMHLAYETLSDGMKRLLDGLKAVNFSAKADTA
                     RTREDRVRSDPTARSAETLLAEHPVVRTHPETGRKAIYVNIAHTVRFVGMTEEESAPL
                     LGYLFQHQIRPEFTCRFAWRPGSLVFWDNRATQHNPINDYHGHRRVMHRITLAGDKPR
                     "
     gene            complement(2368..3651)
                     /locus_tag="VGP50_16490"
     CDS             complement(2368..3651)
                     /locus_tag="VGP50_16490"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEV8029034.1"
                     /translation="MLLAVTALVFIALVALSMPIVFALGIAGIVGLLLGGFPLQMLSS
                     SIVGGSQNWVLLAIPSFIFAGTLMERCGMSHALVQLARALVGWLRGGLGMSVIVVSYF
                     FSDICGSKMAEVSALGSTLMPSLTRAGYRPEDSASLIASGTAMGMLVPPAIFMIVIGD
                     VTNTSVVALFLAGFIPAATIGLCLCALVLLQARLLGWPKDTRPSWSFLVAALREALVP
                     MVVPVVILGGFYFGAFTATEAGAIVAFYAFLAARFFYRNVSWNEMLSIAYESALLTAA
                     VVFLLAVATIFQYLMGVSGAPAFLAATLKPLSGTHWLFLTGVALMTMLFGMVLEGLPA
                     AVVLIPVVFPIATKLGINPVHFDIVQTAAVGIGLFLPPLGVGLLMALRFAQIPVGRHA
                     RAYWPYLLALLSGLMLIICIPELSLFLPRSAGLIR"
     gene            complement(3655..4188)
                     /locus_tag="VGP50_16495"
     CDS             complement(3655..4188)
                     /locus_tag="VGP50_16495"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEV8029035.1"
                     /translation="MSPAAAAPAQRQLAAALKFLLALLLAVAILDMLAGVLLRYVVVR
                     VTDLFDLPSVNFFWVEEVGELALAWLTLLGAAVAVIERTHFALAILTHRLPLGAQLIV
                     ERINHLLMAGFGVLVAVYGWKLAQLNATLLTPALQISLSWLYLSAVAGGVLMALFGLG
                     IASGLIRPRGIESSGAG"
     gene            complement(4203..5225)
                     /locus_tag="VGP50_16500"
     CDS             complement(4203..5225)
                     /locus_tag="VGP50_16500"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEV8029036.1"
                     /translation="MTDTIRRTNRRGFLTAAGGLAGILAAGRTPAFAESVAPKKLIFA
                     HINPPPESAAVAFDWMAQEAGKRSGGALEIEFYGSTLLTKELEIMNAVKAGNVAIGDP
                     AGAAATIFPEMGVFLVPYLVRSYDQAYRLFNGKVGDGLDRQFQERYGVKVLCFFDFGY
                     RHFWNSKRPIVEPKDLRGTKLRVQLAKVFADTVNGLGGNAVPMPWAEVIPAAQSGVID
                     GGDLPIVNILALKAYEVSKYCSMTYHNYGPTVTVINLGVWNDLDKEHQTLLLDLSREA
                     QAIIRRDTESVDNFQKAKELLEPKGMIVNEADVGAFRKLAEEKIWPAYQKQYAELWEE
                     IVKTEA"
     gene            complement(5303..7027)
                     /locus_tag="VGP50_16505"
     CDS             complement(5303..7027)
                     /locus_tag="VGP50_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEV8029037.1"
                     /translation="MSGEATRDGRNEETVAGLLLRRLQAAGIDYFFANAGTDFPPIIE
                     GFARAAETGTALPRPLLIPHENAAVAMAHGVYMVSGRPQAVMVHTAVGTGNTLNALIN
                     ASRDSVPLLLLAGRTPVTERPDLHGARNRHIHWAQEMFDQAGMLREIVKWDYELRRPE
                     QVEDVVARAIEIAMTPPRGPVYLTLPRETLAAGAGAAQQDTRRSPPASAYPDPAAIET
                     LAAWLAAATHPLIITADAGRTDEGAAALARLAQRFALPVVAFNPRFLALPSTHPSHQG
                     HQPRPLLETADLVLVVDCDVPWIPSLESPPEHCRVVHIGEDPNFTRYPMRSFPADLSI
                     AAAPAAALAALDRALAARMEADDATVAARRADLAERSAARRARAAQTAEKDGTLPHIT
                     PEWLSRCIGDAVGADAIIVNEYPLRLDHCARTTPRSYFGSSPAGGLGWGFGAALGAKL
                     AARHRLVVATLGDGAYVFANPTACHWTAAAHDLSILVIVFNNGRYAAVRNATLDMYGE
                     GAAARGKGELLANLTPSPAFEMLVQASGGWGMRVERPAELPSALAGAIDVVTREKRQA
                     LLNVICRY"
BASE COUNT         1325 a         2404 c         2421 g         1147 t
ORIGIN      
        1 gtccaggcga tctcgccttc gccgccgcaa tactattatt gggcggcgcc cgagctcggg
       61 cgcgaggcgg cgcgtctcgt caacaatcgc atcgccgaga tcgtggcgac gcatcccgat
      121 cgtttcgtcg ggctcggcac ggttccgctc caagctcccg acctcgccgt cgcggagctc
      181 gagcggatgg tgaaagaact gggcctgcgt ggcgtcgaaa tctgcacgaa tgtcaatggc
      241 gcggagctct cggagcagcg gtttcgtccg ttctttgcca aggccgagga gctcggcatc
      301 ctcgttttca tgcatcccaa cggcttcacc gaagggcggc ggctcgccga tcactatttc
      361 atcaacgtca tcggcaaccc gctcgactcg acgatcgcgg tgagccatct gattttcggc
      421 ggcgtgctcg acgcccatcc gcgactgaag atctgcgttg cgcatggcgg aggcttcttg
      481 ccggcgtatt caggccgcat ggaccacgcc catggcgctc gttccgactg ccgccgcgag
      541 atcaccaaga agccgacgcg ccatctggcc aagctctatt tcgacaccat cgtctttacg
      601 catgaccagc tcgagtatct ggtccagcgc tggggcagcg atcacattct cttgggcagc
      661 gactatcctt tcgacatggc cgagcccgat cccgtcggct tcgtcagcgg tgcagcgctc
      721 agcggtgacg acaaggcggc gatcctcggg cgcaacgcgg cgaagttgct caagatccgg
      781 atccctgcag cgccgcgtcg caagagttga tccaaggcgc cctcgacagg cgccggttgg
      841 catgcgagcc tcggagaatt cacgggagga cggcatgaag ctctatggct tttggcggtc
      901 gcaggcgacg tttcgcgtcc ggatcgcgct caacctcaaa ggactgaagc gcgaggacgt
      961 cttcatcaat ctcgatcatg gcgaccagca cagcgcggcc tataaggcgc tcaatcctca
     1021 ggcggtgctg ccgacgctgt tcgacggcga cgccaagctc ttccagtcct tcgccatcct
     1081 cgaatacctg gaggagagat acccgaagcc gccgattctc cccgcggacc cgctggcgcg
     1141 cgcctgggtg cgcggcttcg cgctgatcaa tgtcgcggat tcccaccccc tcatcgtgcc
     1201 gcgcatccgc aactacctga ccgatgtgct caaagtcgac gaggccgcca agaccgcctg
     1261 gatccagcac tggctcgcgg ccggactcga ggcgatggag cggctgctcg cggagaagaa
     1321 acccgccggc cgcttctgcc acggcgacag cccgaccgtg gccgacatct gcctgatgac
     1381 gcaggtgacc ccggcgcaga tcttcaaatg caccctcgat ccctatccgc tgaccatgcg
     1441 tatctacgaa acctgcatga gcatccccgc tttcgccgac gcgcatccgc ggaagcagcc
     1501 ggacttcgcg ccgcattgac gccgcatcat cgctcatgac gctcgacatc cggccgattg
     1561 ccggcgcgct cggagccgag atccacggca tcgatctcga cgtcgggctc gatgcgcaga
     1621 cgatcgccgc catccgccag gcgttgctcg atcacctcgt catctttttc cgcgagcagc
     1681 gtctgccacc cgaccggttc ctcgctttcg cgcgccgctt cggccggccg gtcgaatatc
     1741 ccttcgtcaa gggcatcgag ggccatcccg agatcatcgc cgtgaccaag cgcgaagatg
     1801 agcgcgtgaa tttcgggggc atatggcaca gcgacacgac ctatctggcg gagccgccga
     1861 tggggacgat gctgatcgcg cgcgaggtgc caccctatgg cggagacacg ctcttcgcca
     1921 acatgcacct cgcttacgag acgctctccg acggaatgaa gcggctcctc gacgggctca
     1981 aagcggtgaa cttctcggcc aaggccgaca cggcgcgcac gcgcgaggat cgcgtgagat
     2041 ccgacccgac ggcgcgttcc gccgaaactt tgctggccga gcacccggtg gtgcgcaccc
     2101 atcccgagac cgggcgcaag gcgatctatg tcaatatcgc gcacacggtc cgcttcgtcg
     2161 gcatgaccga ggaagagagc gctccgcttc tcggctatct cttccagcat cagatccggc
     2221 ccgagttcac ttgccgcttc gcctggcggc ccggctcgct cgttttctgg gacaaccgcg
     2281 ccacgcagca caaccccatc aacgactatc acggccatcg ccgcgtgatg catcgcatca
     2341 cgcttgccgg cgacaagccg cgctgactca acggatgagc ccggcgctcc gcggcaggaa
     2401 gagcgagagc tcggggatgc agatgatcag catcagtccc gagagcagcg ccaggagata
     2461 aggccaatag gcacgggcgt ggcgcccgac cggaatctgg gcgaagcgca gggccatcag
     2521 cagcccgacg cccagcggcg ggaggaacag gccgatgccg acggccgccg tctgcacgat
     2581 gtcgaaatgc acgggattga tgccgagctt ggtggcgatc gggaacacca cggggatcag
     2641 cacgaccgcc gccggcaggc cttcgaggac catgccgaac agcatggtca tgagggcgac
     2701 gccggtgagg aaaagccaat gggtccccga gagcggcttc agcgtcgccg caaggaatgc
     2761 cggtgcgccg ctcacaccca tcagatattg gaagatggtc gcgaccgcca gcaggaacac
     2821 gaccgccgcc gtcagcaacg cgctctcata ggcaatcgac agcatctcat tccaggagac
     2881 gttgcgatag aagaaccgcg cggcgagaaa ggcgtagaac gcgacgatgg cgccggcttc
     2941 ggtggcggta aaggcgccga agtagaagcc gccaaggatc accactggca ccaccatcgg
     3001 caccagcgcc tcgcgtagcg ccgccacgag aaaggaccag cttggccgcg tgtccttggg
     3061 ccagccgagc agccgcgcct gcaacaagac gagcgcgcag aggcagaggc cgatggtggc
     3121 ggccgggatg aagccggcaa gaaacagcgc cacgaccgac gtattggtca cgtcgccgat
     3181 gacgatcatg aagatcgcgg gcggcaccag catgcccatg gccgtgcccg aggcgatcag
     3241 cgacgcgctg tcctcgggac ggtagccggc acgggtcaag gagggcatca gcgtcgatcc
     3301 aagcgccgaa acctcggcca tcttggagcc gcagatgtcc gagaagaaat aggagacgac
     3361 gatcaccgac atgccgagtc cgccgcgcag ccagccgacg agcgcgcggg cgagctgcac
     3421 cagggcatgc gacatgccgc agcgctccat gagcgtgccg gcgaagatga aggacgggat
     3481 cgccagcagc acccagttct gcgagccgcc gacgatgctc gatgacagca tctgcagcgg
     3541 aaagccgccg agcaggaggc cgacaatgcc ggcgatgccc aacgcgaaca cgatcggcat
     3601 ggacaacgcg accagagcta tgaagacgag ggcagtgacc gccagcagca tggctcagcc
     3661 ggcgccgctg gactcgatcc cgcgcgggcg tatcaggccg gaggctatgc cgagcccgaa
     3721 caacgccatg aggacaccgc cggcgacggc gctcagatag agccaggaaa ggctgatctg
     3781 gagcgctggc gtcaggaggg tggcgttgag ctgcgcgagc ttccagccat agaccgcgac
     3841 cagaacgccg aagccggcca tcagcagatg attgatgcgc tcgacgatga gttgggcgcc
     3901 aagcggcagg cgatgcgtga gaatcgccag cgcaaaatgc gtccgctcga tgacggcgac
     3961 ggcagcgccc agcagcgtga gccaggcgag cgcgagctcg cccacttcct cgacccagaa
     4021 gaagttcacg gatggcaggt cgaagagatc cgtgacgcgc acgacgacat agcgcaacag
     4081 cacgcccgcc agcatgtcga gaatcgccac cgccagaagc agcgccagga gaaacttcag
     4141 cgcggcggcg agctggcgct gcgccggtgc cgctgctgcc ggactcatcg tcggcacgcg
     4201 gctcaagcct cggtcttgac gatctcttcc cagagctcgg cgtattgctt ctgataggcg
     4261 ggccagatct tctcctcggc gagcttgcgg aaggcgccga catcggcctc gttgacgatc
     4321 atccccttgg gctcgagcag ctctttggct ttctggaaat tgtcgacgct ctccgtgtcg
     4381 cggcggatga tcgcctgcgc ctcgcgcgag aggtcgagga gcagcgtctg atgctccttg
     4441 tcgagatcgt tccacacgcc gagattgatg accgtcaccg tcggcccgta attgtgatag
     4501 gtcatcgaac agtatttcga cacctcatag gccttgagcg cgaggatgtt gacgatcggg
     4561 agatcgccgc cgtcgatgac gccgctctgc gccgccggga tcacctcggc ccagggcatc
     4621 ggcacggcgt tgccgccgag gccgttgacg gtgtcggcga ataccttggc gagctgcacg
     4681 cggagctttg tgccgcgcaa atcctttggc tcgacgatcg gacgtttgct gttccagaaa
     4741 tggcggtagc cgaaatcgaa gaagcagagc accttgacgc cgtatctttc ctggaactgc
     4801 cgatcgaggc cgtcgccgac cttgccgttg aacaggcgat aggcctgatc atagctgcgc
     4861 acgaggtagg gaacgaggaa cacgcccatc tccggaaaga tggtcgcggc ggcgcccgcg
     4921 ggatcgccga tggcgacatt gccggccttc accgcattca tgatctcgag ctctttggtg
     4981 aggagcgtgc tgccatagaa ctcgatctcg agcgcgccgc ccgaccgctt gccggcttcc
     5041 tgggccatcc agtcgaaagc caccgcggcc gattccggcg gtggattgat atgcgcgaag
     5101 atcagtttct tgggcgcaac gctctcggca aaagccggcg tccggccggc cgcaagaatg
     5161 ccggcaagcc cacccgcggc ggtgaggaaa ccacgacggt tcgtccgacg aattgtgtcg
     5221 gtcatctgct cctcccctgg atttgcggcg ttgtgccgct ttatttgcgg ggatgatagc
     5281 agattgcggc atctttgcgc cgtcaataac ggcagatcac gttgagcagc gcctgccgct
     5341 tctcgcgcgt aacgacatcg atcgcaccgg cgagcgccga aggcagctcc gccggccgct
     5401 ccacgcgcat gccccagccg ccactcgcct ggaccagcat ctcgaaggca gggctcggcg
     5461 tcagattggc caggagctcg cccttgccgc gtgccgccgc gccctcgcca tacatatcga
     5521 gcgtcgcatt gcggacggcc gcgtagcggc cgttgttgaa gacgatgacg aggatcgaca
     5581 aatcatgcgc tgccgcagtc cagtggcagg ccgtcgggtt ggcgaagacg taagcgccgt
     5641 cgccgagtgt cgctaccacg aggcggtggc gcgcggcgag ctttgcgcca agcgcggcgc
     5701 cgaagcccca gccgagaccg ccggcggggc tcgagccgaa atagctgcgg ggcgtcgtgc
     5761 gcgcgcaatg atcgaggcgt agcggatatt cattgacgat gatcgcgtcc gcgccgacgg
     5821 cgtcgccgat gcagcggctg agccattcgg gggtgatgtg cggcagcgtc ccgtcctttt
     5881 ccgccgtctg cgcggcgcgg gcgcgccgcg cggccgagcg ttcggcgaga tctgcccgcc
     5941 gcgcggcgac cgtggcatcg tccgcttcca tccgcgcggc gagcgcgcga tcgagcgcgg
     6001 ccaacgcggc ggccggggca gccgcgatgg aaagatcggc cgggaagctg cgcatcggat
     6061 accgcgtgaa gttcgggtcc tcgccgatgt gaacgacgcg gcaatgctcg ggtgggctct
     6121 cgagcgacgg gatccacggc acgtcgcaat cgaccacgag gacgaggtcc gccgtctcga
     6181 ggagcggccg cggctggtgc ccctgatgcg acgggtgggt gctcggcagc gcgaggaagc
     6241 gcgggttgaa cgcgacgacg ggcagggcga aacgctgggc aagccgcgcc aatgccgcag
     6301 caccttcgtc cgtgcggccg gcatcggccg tgatgatcag cggatgcgtg gccgctgcca
     6361 gccatgccgc aagggtctcg atcgcggccg gatcgggata ggcggatgcc ggcggtgagc
     6421 ggcgggtgtc ctgttgggcg gcgccggcgc cggcggccag cgtttcgcgc ggcagcgtca
     6481 gatagaccgg gccgcgcggc ggcgtcatgg cgatctcgat ggcgcgcgcg acgacgtctt
     6541 cgacctgttc cggccggcgc agctcgtaat cccatttcac gatctcgcgc agcatgccgg
     6601 cttggtcgaa catctcctgc gcccagtgga tatggcggtt gcgggcgcca tgcaggtcgg
     6661 gacgctcggt gaccggcgtc cgcccggcga ggagcagcag gggcacgctg tcccggctgg
     6721 cgttgatcaa ggcattgagg gtgttgccgg tgccgacggc ggtgtggacc atcaccgctt
     6781 gcggtcggcc cgagaccatg tagacgccat gggccatggc aacggcggcg ttctcatggg
     6841 ggatcagcag cggtcgcggc agcgctgtgc cggtctcggc agcgcgcgcg aagccttcga
     6901 tgatcggcgg gaaatcggtg ccggcattgg cgaagaaata atcgatgccc gccgcctgga
     6961 gacggcgcag caacaggccg gccaccgttt cctcgttccg gccgtcgcgc gtcgcttcgc
     7021 cgctcatcat ccgctcctgt cggcctgccc gactgtccgc cggctccggt tacccgtcaa
     7081 gcggcgcgcg cgaacgttgc cgatgcgcct catgcgcgag ggccggcggt ccacgctcgc
     7141 tcttggagga aagcggaacg gtcgaaggca gcccggggcc gtcgccgtcg gcgacgcccg
     7201 gctcgatgaa ctcgcccacc gggagcgcag gcggcccggc tcagaccacc atcttacggc
     7261 aatagaaggc ttcggcgagt ggcgcgttgc agccgat
//
LOCUS       DASYNS010000536         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4657795, whole genome shotgun sequence.
ACCESSION   DASYNS010000536 DASYNS010000000
VERSION     DASYNS010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4657795"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VGP50_16510"
     CDS             complement(<1..1011)
                     /locus_tag="VGP50_16510"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV8029038.1"
                     /translation="MNGAESLVRTLVASGVEVCFTNPGTSEMNFVAALDKVAELRPIL
                     GLFEGVVTGAADGYGRMAGKPAATLLHLGPGLGNGLANLHNARRAQTPIVNIVGDHAT
                     YHAQYDAPLASDVMGIARPVSGWVHSATSAKTVASDGARAVQAARQAPGQIATLILPA
                     DTAWLEAARPAPPLPVTGPARVDTAAIDQVVDALRGGKKTTLLIGGAALRRRGLETAG
                     RIAAKTGARLTCDTFTARLERGAGLVALERIPYFAEQIVEFMKDTALLVLVGTKPPVS
                     FFAYPGRPSWCTPEGCRILYLTQPHEDGVAALEAVAEALGAPREPAGRAQLNKPELST
                     GYI"
     gene            complement(1028..2194)
                     /locus_tag="VGP50_16515"
     CDS             complement(1028..2194)
                     /locus_tag="VGP50_16515"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8029039.1"
                     /translation="MDLELSDEQKMIVATVRRFVREEILPLEAKLDPDASELPEEDFQ
                     RLSAKVKTMGFWGIDIPEEYGGPGIDLTTRTLMAVEMAQHRAGLYAPCYGVFGGAGLA
                     QLYEASEDQKQRYLYPTLRGEKHGFFGLTEPSGGSDPARAIETRAVKEGDDWVLNGAK
                     IFISGADKADYGLVFARTDKSKGRDGVTCFIVERGMPGFHVRRVVHTLRSTYYATELQ
                     FEDLRVPQRNVLGEVNRGFAIANDRLTRQRIPYAAGCIGIAVKAQEMAIDYAKLRQTF
                     GAPLATRQAIQWMIVDNEIDIRTSLWLVLAAAHKAERGEPYRTEAAMAKLVASEAGGR
                     VVDRAMQIHGGYGMTKDLPLERWYREMRIRRIGEGPSEVQRHIMARDLLGGSFH"
     gene            complement(2175..2543)
                     /locus_tag="VGP50_16520"
     CDS             complement(2175..2543)
                     /locus_tag="VGP50_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029040.1"
                     /translation="MAIRLTKPWQELTADAIRALPGQLGVYQLADGEGRVLYIGYAGG
                     RSLFGLRSAIAEARARLGASARRFRYEVNQQYMSRYEELLMLHVADHGALPSETRDAP
                     RKLGRLSPVAAGKDDGSRAQ"
     gene            complement(2528..>3060)
                     /locus_tag="VGP50_16525"
     CDS             complement(2528..>3060)
                     /locus_tag="VGP50_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029041.1"
                     /translation="AKDIISIGYLKDPTDPQWQEDPGFRDWLAFMRQDYPEGSIADNY
                     NVSGYSLAQTLVEVLRRCGDDLSRERVMKEAAHLDLALPMLLPGIRIKTAPDDFRPLK
                     GDAPRALQRPDLDALHPRHQRLAMTVRALEPPTLTLPLKGGGEFSATAPTSPSPLEGE
                     GGVGGKQQARRSWPSA"
BASE COUNT          465 a         1021 c         1079 g          495 t
ORIGIN      
        1 gatgtaaccg gtggagagct cgggcttgtt gagctgggcg cggccggcgg gctcgcgcgg
       61 cgcgccgagc gcctcggcga cggcttcgag tgccgcgacg ccgtcctcat gcggctgcgt
      121 cagatagagg atccggcagc cttcgggcgt gcaccagctg gggcggccgg gataagcgaa
      181 gaaagagacg ggcggcttgg tgccgacaag gacgaggagc gccgtgtcct tcatgaactc
      241 gacgatctgc tcggcgaaat agggaatgcg ctcgagcgcg acgagcccgg cgccgcgctc
      301 gaggcgcgcg gtgaaggtat cgcaggtgag ccgcgcgccg gtctttgccg cgatgcggcc
      361 cgccgtttcg aggccgcggc gacggagcgc cgcgccgccg atcagcagcg tcgtcttctt
      421 gccgccgcgg agtgcgtcca cgacctggtc gatggcggcg gtgtcgacgc gcgccggacc
      481 cgtgacggga agcggcggcg ccgggcgagc cgcttcgagc caggccgtgt cggccgggag
      541 aatgagcgtc gcgatctggc cgggcgcctg gcgcgccgcc tgcacggcgc gcgcgccgtc
      601 cgacgcgacc gtcttcgcgg aggtggcgct gtgcacccag cccgacaccg gccgggcgat
      661 gcccatgacg tcggaggcga gcggcgcgtc atattgcgcg tggtaggtgg cgtggtcgcc
      721 gacgatgttg acgatcggcg tttgcgcgcg ccgcgcgttg tgcagattgg caaggccgtt
      781 gccgaggccc gggcccagat gcagcagcgt cgcggccggc ttcccggcca tgcggccata
      841 gccgtcggcc gcgccggtca ccacgccctc gaagaggccg aggatcggcc gcagctccgc
      901 gaccttgtcg agcgcggcga cgaaattcat ctccgacgtg ccgggattgg tgaagcacac
      961 ctcgactccc gaagcgacga gcgtccgcac caggctttcc gcgccattca tccggatcct
     1021 ccgtcgctca atggaaggag ccgccgagca ggtcgcgcgc catgatgtgg cgctgcacct
     1081 cgcttgggcc ctcgccgatg cggcggatgc gcatctcgcg ataccagcgc tcgagcggca
     1141 ggtccttggt catgccgtag ccgccatgga tctgcatggc gcgatcgacg acgcggccgc
     1201 ccgcctcgct cgccaccagc ttcgccatgg ccgcctcggt gcggtagggc tcgccgcgct
     1261 cggccttgtg cgcggccgcc agcaccagcc agagactggt gcggatgtcg atctcgttgt
     1321 cgacgatcat ccactggatc gcctgccgcg tggcgagcgg cgcgccgaag gtctggcgca
     1381 gcttggcata gtcgatggcc atctcctgcg ccttgacggc gatgccgatg cagcccgcgg
     1441 cataagggat gcgctggcgc gtgaggcggt cgttggcgat ggcgaagccg cgattgacct
     1501 cgcccagcac attgcgctgc ggcacgcgca gatcctcgaa ttgcagctcg gtcgcgtaat
     1561 aggtggagcg cagcgtatgg acgacgcggc gcacatggaa acccggcatg ccgcgctcga
     1621 cgatgaagca ggtgacgccg tcgcggccct tgctcttgtc ggtgcgcgcg aaaacgaggc
     1681 cgtaatccgc cttgtcggca ccgctgatga agatcttggc gccattcagc acccaatcgt
     1741 cgccctcctt caccgcgcgc gtctcgatgg cgcgcgcggg atcgctgccg cccgaaggct
     1801 cggtgaggcc gaagaagccg tgcttctcgc cgcgcagcgt cggatagaga tagcgctgct
     1861 tctggtcctc gctggcttcg tagagctgcg ccaggcctgc gccgccgaag acgccgtagc
     1921 agggcgcata gagcccggcg cgatgctgcg ccatctcgac ggccatgagc gtgcgcgtcg
     1981 tgagatcgat gccgggcccg ccatattcct cgggaatgtc gatgccccag aagcccatcg
     2041 tcttgacctt ggcggagaga cgctggaaat cctcctccgg caactcgctc gcatcgggat
     2101 cgagcttggc ctcgagcggc aggatctcct cgcgcacgaa gcggcgcacg gtcgcgacga
     2161 tcatcttctg ttcgtcactg agctcgagat ccatcgtcct tccctgccgc gaccgggctc
     2221 aagcgcccga gcttgcgcgg cgcatcgcga gtctcggacg gcagcgcgcc gtgatcagcg
     2281 acgtgcagca tcagcagctc ttcatagcgg ctcatatatt gctggttcac ctcgtagcgg
     2341 aagcgccggg cggaagcgcc gagccgcgcc cgcgcctccg cgatggcgct gcgcaggccg
     2401 aagagcgagc gcccgccagc atagccgata taaaggacgc ggccctcgcc gtcagcgagc
     2461 tgatagacgc cgagctggcc cggcagcgca cggatggcgt ccgccgtcag ctcctgccac
     2521 ggcttggtca ggcggatggc catgaccgcc gtgcctgttg cttacccccc acccctccct
     2581 ccccctcaag gggggagggg gaagttggtg cggtggcaga gaattcccct ccccccttga
     2641 gggggagggt cagggtgggg ggttccaggg cgcggacagt catcgctagc cgctgatgac
     2701 gggggtgaag ggcatccaga tctggccgtt gaagcgctcg aggcgcatct cctttcagtg
     2761 ggcggaaatc atcgggtgcg gtcttgatcc ggatgcccgg cagcagcatc ggcaacgcca
     2821 ggtcgagatg ggccgcctct ttcatgacac gctcgcgcga aaggtcgtcg ccgcagcgcc
     2881 tcagcacctc gaccagcgtc tgcgccagcg aatagccgga gacgttgtag ttgtcggcga
     2941 tgctgccctc gggatagtcc tggcgcatga aggcgagcca gtcgcgaaag cccggatcct
     3001 cctgccattg cgggtcggtc ggatccttca gatagccaat gctgatgatg tccttggcct
//
LOCUS       DASYNS010000537         4174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2944782, whole genome shotgun sequence.
ACCESSION   DASYNS010000537 DASYNS010000000
VERSION     DASYNS010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2944782"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="VGP50_16530"
     CDS             <1..1668
                     /locus_tag="VGP50_16530"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEV8029042.1"
                     /translation="LAKGKINLEILNPEPFSPAEDRAVAFGLQGVPIDQGGEQVYFGL
                     AASNSTDDQQTIPFFQPDRERFLEYDLTKLIHGLAVPKKTVVGLITSLPLEGDFMAAM
                     QGRPVEPYAIIDQLRQLYELRTLSTNLDQVGDDVDVLMIVHPQNLPEKTLYAIDQFVL
                     KGGKALVFVDPDSETQAAHAGRMSPPGSPTASNLEKLFTAWGVSFDEGKVAGDLRDAR
                     RVNAGTQGHAQALEYVAWLALNEDNLNRDTAITGDLSRINVATAGILSPRDGAKTSFT
                     PLITTSEESEEIPVDKVEGFPDVAGLLRDFKSDGKKLTLAAEIVGPADTAFPDGPPKA
                     DTSDKDKDKDKDDAKAGEAKDAADAAAQIKTAAQPINVVVVADTDMLDDRFWVQEQDF
                     FGQRVTVPSAGNGDFVANAIDVLSGGNDLVSLRTRGTSARPFEVVQNIQRAADERYQA
                     RAKALEQELKDTQAKIKELQGKEGTAASVTPAAEQSQAIENFRTQMLQTRQQLRQVQL
                     FLRQDIDRLKAKLEFFDIAFVPLLVGLIAVILGAWRLRWRRRHAHAH"
     gene            1682..2710
                     /locus_tag="VGP50_16535"
     CDS             1682..2710
                     /locus_tag="VGP50_16535"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEV8029043.1"
                     /translation="MKPWGFLALVGVTAAAVVAAVLLSLWPVGPSADRGPGAAVLPGF
                     AQAAEQADHVVLVHGDARITLVRQGDKWAVEEKSLYPVDTGKLRQLFLGLAELRYVEP
                     KTKKPELYARLEVEDAGKPDSKSTLVTVGAEKNGVLAALILGQRRTDALGGGVDGIYL
                     RKPDDAQSWLARGSMELPSDIAAWLDRKLVDIGEDKIKEVVLTQPDGAKLDIARDKPE
                     DKFALKDPPADAKIKSDAPIDEPARALATLELADVRAAADMPFPAEGVGHAVYTTFDG
                     LTIDIALLDKDSKHWARFSAQGTGDAEKTASELNERLSPWVYALLDYKAKLLTTKLSD
                     LTAPAKGS"
     gene            2707..2916
                     /locus_tag="VGP50_16540"
     CDS             2707..2916
                     /locus_tag="VGP50_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HEV8029044.1"
                     /translation="MSDGDPASPCTAVCILDPASGLCRGCFRTIDEITRWPSLGADAK
                     RRILAELPGRRPPPGRLAEAPAAPT"
     gene            2954..3793
                     /locus_tag="VGP50_16545"
     CDS             2954..3793
                     /locus_tag="VGP50_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEV8029045.1"
                     /translation="MTRPRPVARLARGTPASDGAGVKLNRVIGTATLPAVDPFLMLDE
                     FRSDDAADYIAGFPDHPHRGFETVTYMLAGRMRHRDNKGHEGLLEAGSVQWMTAGRGI
                     VHSEMPEQENGLMQGFQLWVNLPAKDKMTAPRYQEIPPRDIPVAELPGGVRVKVIAGA
                     LGSVQGPINGIAIEPVLLDVALPAGARFAMPLPSGHNVFLYVFEGKAAVGGDGAAVPR
                     GSIAVLGEGDGVALAAPEGAARVLLAAGRPLNEPVARYGPFVMNTEQQLREAVRDFQL
                     GRL"
     gene            complement(3801..4079)
                     /locus_tag="VGP50_16550"
     CDS             complement(3801..4079)
                     /locus_tag="VGP50_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029046.1"
                     /translation="MPLHRLLLAAALLLPLLGCNVWENHAEFAPPESRWPSTLPSASL
                     ANAPPPPIGLEYCYRTLASVDCFNEPKPERVMGYTGIYPEPVPVAPPR"
BASE COUNT          762 a         1417 c         1405 g          590 t
ORIGIN      
        1 ctcgccaagg gcaagatcaa cctcgagatc ctcaatcccg agcccttctc gccggccgag
       61 gatcgtgccg tcgctttcgg cctccagggc gtgcccatcg accagggcgg agagcaggtc
      121 tatttcgggc tcgccgccag caattccacc gacgaccagc agaccatccc gttcttccag
      181 cccgaccgcg agcgcttcct cgaatatgat ctgacgaagc tcatccacgg cctcgcggtg
      241 ccgaagaaga cggtggtggg actcatcacc agcctgccgc tcgaaggcga cttcatggcg
      301 gcgatgcagg gacggccggt cgagccctat gccatcatcg atcagcttcg ccagctctac
      361 gagctgcgca cgctgtcgac caacctcgac caggtcgggg acgacgtcga cgtcctgatg
      421 atcgtccatc cgcagaacct gccggagaag acgctctacg ccatcgacca gttcgtgctc
      481 aaaggcggca aagcgctggt cttcgtcgat ccggattccg aaacgcaagc cgcccatgcg
      541 ggccgcatga gcccgccggg cagcccgacc gccagcaatc tcgagaaact gttcacggcc
      601 tggggcgtct cgttcgacga gggcaaagtg gccggcgacc ttcgcgatgc gcggcgcgtc
      661 aacgccggca cgcaagggca cgcacaggcg ctcgaatacg tcgcctggct cgcgctcaac
      721 gaggacaatc tcaatcgcga caccgcgatc accggcgatc tgtcgcggat caatgtcgcg
      781 acggccggaa tcctgtcgcc gcgcgacggc gccaagacca gcttcacgcc gctcatcacg
      841 acctcggagg agtccgagga gatcccggtc gacaaggtcg aaggctttcc ggacgtcgcg
      901 gggctgctgc gcgacttcaa atccgacggc aagaagctga cgcttgccgc cgagatcgtc
      961 ggccccgccg acaccgcctt ccccgacggc ccgcccaaag ccgacacgtc cgacaaggac
     1021 aaggataaag acaaggacga cgcgaaggcg ggcgaggcga aggacgcggc cgacgccgcc
     1081 gcgcagatca agacggcggc acagccgatc aacgtcgtcg tcgtcgcgga cacggacatg
     1141 ctcgacgacc gcttctgggt gcaggagcag gatttcttcg gccagcgcgt cacggtgccg
     1201 tcggccggca atggcgactt cgtcgccaac gcgatcgacg tgctgtcggg cggcaacgat
     1261 ctcgtcagcc tgcgcacccg cggcacctcg gcccggccct tcgaggtcgt gcagaacatc
     1321 cagcgcgccg cagacgagcg ctatcaggcg cgggccaagg cgctcgagca ggagctcaag
     1381 gacacccagg cgaagatcaa ggagctgcaa ggcaaggagg ggaccgcggc gagcgtcacg
     1441 ccggccgcgg agcaaagcca ggcgatcgag aatttccgca cccagatgct gcagacgcgc
     1501 cagcagctgc gccaagtgca gctcttcctg cgccaggaca tcgaccgcct gaaggcgaag
     1561 ctcgaattct tcgacatcgc tttcgtgccg ctgctggtcg gcctgatcgc cgtcatcctc
     1621 ggtgcgtggc ggctgcgctg gcggcgccgc cacgcccacg cccattgagc gaggaagcgc
     1681 gatgaagcct tggggatttc tggctctggt cggcgtgacc gccgcggccg tcgttgcagc
     1741 ggtgctgctg tccctctggc ctgtcggccc gtccgctgat cgcggaccgg gcgccgccgt
     1801 gctgccgggc ttcgcccagg cggcggagca ggcggaccat gtcgtgctgg tacatggcga
     1861 cgcgcgaatc acgctggtgc ggcaaggcga caaatgggcc gtcgaggaaa agagcctcta
     1921 tcccgtggat acgggaaagc tgcgccagct cttcctggga ctggcggagc tgcgctacgt
     1981 cgagcccaag acgaagaagc ccgagcttta tgcgcggctc gaagtagagg atgccggaaa
     2041 acccgacagc aaatcgacac tcgtcacggt gggcgccgag aagaacggcg tgcttgccgc
     2101 gctgatcctc gggcagcggc gcaccgatgc gctgggcggc ggcgtcgacg gcatctatct
     2161 gcgcaagccc gatgatgcgc agtcctggct cgcccgcggc agcatggagc tgccgagcga
     2221 catcgccgcc tggctcgacc gcaagctcgt cgacatcggc gaggacaaga tcaaagaggt
     2281 ggtgctgacc cagcccgacg gcgccaagct cgacatcgcc cgcgacaagc ccgaagacaa
     2341 gttcgcgctc aaggatccgc cggccgacgc caagatcaag agcgatgccc cgatcgacga
     2401 gccggctcgc gcactggcga cgctcgagct cgccgatgta cgcgccgccg cggacatgcc
     2461 cttccccgcc gaaggcgtgg gccacgccgt ctacacgacc ttcgacggat tgacgatcga
     2521 catcgcgctc ctggacaaag acagcaagca ctgggcgcgc ttttcggcac agggcacggg
     2581 cgatgccgaa aagacggcga gcgagctcaa cgagaggctc tcgccctggg tctatgcgct
     2641 cctcgattac aaagccaagc tgctcaccac gaaactctcc gatctgacgg cgccggccaa
     2701 gggatcgtga gcgacggcga tcccgcctcg ccctgcaccg cggtctgcat ccttgacccg
     2761 gcgagcggcc tctgccgcgg ctgcttccgc accatcgacg agatcacgcg gtggccgagc
     2821 ctcggcgccg atgcgaagcg ccgcatcctc gccgagctgc cgggacgacg gccgccgccc
     2881 ggccgcttgg cagaagcccc ggcggcgccc acataaggcg aaatctgacg gaggatcggc
     2941 gatggatggc ggaatgacac ggcctcggcc ggtggcgcgg ctggcccgcg gcacgccggc
     3001 cagcgacggg gcgggcgtca agctcaaccg ggtgatcggc acggcgacgc tgccggcggt
     3061 cgacccgttc ctgatgctcg acgagttccg ctcggacgac gccgccgact acatcgccgg
     3121 cttcccggac catccccatc gcggcttcga gaccgtcact tacatgctgg cgggccgcat
     3181 gcgccaccgc gacaacaagg ggcatgaggg cctcctcgag gccggcagcg tgcaatggat
     3241 gaccgccggc cgcggcatcg tccattccga gatgccggag caggagaacg gcctgatgca
     3301 gggcttccag ctctgggtca acctgcccgc caaggacaag atgacggcgc cccgctacca
     3361 ggaaatcccg ccccgggaca tcccggtcgc ggagctgccc ggcggcgtcc gcgtcaaagt
     3421 gatcgccggc gcgctcggta gcgtgcaagg tcccatcaac ggcatcgcca tcgagccggt
     3481 tctgttggat gtggcgctgc cggcgggtgc gcgtttcgcg atgccgctgc cgagcgggca
     3541 caatgtcttc ctctatgtct tcgaaggaaa agcggcggtc ggcggcgatg gcgcggcggt
     3601 gccgcgcggc agcatcgccg tgctcggcga gggcgacggt gtcgcgctgg cggcgccaga
     3661 gggcgccgcg cgcgtgcttc tcgcggccgg gcggccgctc aacgagccgg tggcccgcta
     3721 cgggcccttc gtcatgaaca cggagcagca gctgcgcgag gcggtgcgcg actttcagct
     3781 cggcaggctc tgaagcgagc tcagcggggc ggcgctaccg gcacgggctc gggatagatg
     3841 ccggtatagc ccatgacgcg ctcgggcttc ggctcgttga agcaatcgac actggcgagc
     3901 gtgcgatagc agtactcgag gccgatcggc ggcgggggag cgttcgccaa cgacgccgac
     3961 ggaagggtcg agggccaacg gctttccggc ggggcgaatt cggcgtggtt ctcccagaca
     4021 ttgcagccga ggagcggcaa cagcagagcg gcggcgagga ggagacggtg caatggcatc
     4081 ggaaggtcct ccgcccatct ataggcagcg cgagttaatc ttccgtatag tagaggccgg
     4141 ctaactttcc ggcgaaagca gaggagaata ccca
//
LOCUS       DASYNS010000538         6523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5127023, whole genome shotgun sequence.
ACCESSION   DASYNS010000538 DASYNS010000000
VERSION     DASYNS010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5127023"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /gene="cysS"
                     /locus_tag="VGP50_16555"
     CDS             complement(<1..746)
                     /gene="cysS"
                     /locus_tag="VGP50_16555"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV8029047.1"
                     /translation="MPLALYNTLTRRKEPFAPLDAANVRMYVCGPTVYDFAHIGNARP
                     VIVFDVLYRLLRHLYGAKHVRYVRNITDIDDKIIAAAKENDERIETLTQRTTRTFEDD
                     AAALGALKPDVEPRATEHLAPMIAMIERLIAVGHAYVAEGHVLFAVSSMPDYGKLSRR
                     SHDEMIAGARVEIAPYKRDPADFVLWKPSTPDQPGWESPWGRGRPGWHIECSAMSETY
                     LGETFDIHGGGLDLIFPHHENEIAQSVCSHR"
     gene            complement(848..1387)
                     /locus_tag="VGP50_16560"
     CDS             complement(848..1387)
                     /locus_tag="VGP50_16560"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8029048.1"
                     /translation="MIFIDDADAIFEDGEERGLYRYLLTMLDGLESEGSSRVCVMMTA
                     MNLRHLPPALVRSGRVELWLEMKLPDREARQRILENHLRGVPREVAECDVDRILAATD
                     GFTGADMKRLVDDAKGLYTFGRSTGAAARGATDYFIEAATGVRENKRRYEEAELAAQT
                     RPRGRPMGFFYGAPPAHED"
     gene            complement(1371..1976)
                     /locus_tag="VGP50_16565"
     CDS             complement(1371..1976)
                     /locus_tag="VGP50_16565"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8029049.1"
                     /translation="MSTDGSIAQAFATRSFSASIRRYSAGDYAALLANLVGDEQAAAI
                     DAQKIYRFAPKLNAHQLRAAADWLRTSGSLSTDGIIQYLRSQRLASNVDLTEVQEVDL
                     HDLLGVDDVVRSLEIHVALPLNNDSLTAEFGLRPKRGVLLYGPPGSGKTTIGRALAHR
                     LRGKFVLIRRHLHRRNQRLLRAHPPRLRSGQRQLAVCDLHR"
     gene            complement(2151..2795)
                     /locus_tag="VGP50_16570"
     CDS             complement(2151..2795)
                     /locus_tag="VGP50_16570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8029050.1"
                     /translation="MSQPTGPLLDPKVNAVISRLQAARRRPADGGPLGNPDNEQDPHA
                     YADYGFSIHPEQGELIYLLCRAQGATRVAEFATSVGMSTLYFATAVRDNGGGVVIGSE
                     LVPEKVAAARRNLAEAGLAGLVDLRQGDARETLRVLGGPIDFMLVDGWPGGEGPSLAR
                     QVMEIVAPQIRLGGLVMNDNGEADYLAYVRDPANGFRSMSLPLKGGTELSVKVA"
     gene            complement(2828..3478)
                     /locus_tag="VGP50_16575"
     CDS             complement(2828..3478)
                     /locus_tag="VGP50_16575"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV8029051.1"
                     /translation="MFYDIGQDYRRTGPLVRFMARHRGGRGFGHFAAGFMGGGRGGAG
                     FRTGRKLTSADLQLIILALLLERPSHGYELIKALEERSGGFYSPSPGVIYPALTFLEE
                     IGYATVETESTRKLYRVTDAGRAHLEQNRSLVDAMLAEFERIGGKMEHVRRVFAGEET
                     AGDAEDDGLGPRGSAELQAARRALKRALREKSDGSPQETKRIAEILRRAAADILGK"
     gene            complement(3551..5284)
                     /locus_tag="VGP50_16580"
     CDS             complement(3551..5284)
                     /locus_tag="VGP50_16580"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014239482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEV8029052.1"
                     /translation="MIASPDSLTIAIAQLDPTVGDIAGNLAKLRQAMDEAAQLGADLV
                     VASELYVAGYPPEDLVLKPAFVAACQAAVREFAKATTRGPAVILGTPWRQDGKLYNAA
                     ALLENGWISALRFKHELPNYGVFDEKRVFAAGPAPGPISFHGVRLGLMICEDMWQPDA
                     TEALQESGAQLLIVINGSPYESDKVDERLTLAHQRVTESGLPLLYVNQVSGQDELVFD
                     GASFALDVAGELCVQAPNWLETVVPTVWRRGADDRWTVESGKLVSPSEGLEATYQAMV
                     LGLRDYVRKNRFPGVVLGLSGGIDSALTAAVAVDALGPEQVHCVMMPSPFTSAESVDD
                     AAEVAQLLGVDLWTISIEPAMQAFEAMLRLTFEGREKDVTEENIQSRARGMTLMALSN
                     KFGWMVLSTGNKSEMSVGYATLYGDMCGGYAVLKDVYKMTVFALSGWRNRTKPDNLLG
                     PAGPVIPERVLTKPPSAELKANQTDQDTLPPYEVLDAILECLVEKELAVPEVVARGHD
                     EATVRMVWRMLDRAEYKRRQAPPGVKLTRRAFGRDRRYPVTNAFQDKAPAPGQVQEPG
                     ETPRAPAPSAT"
     gene            complement(5319..6449)
                     /locus_tag="VGP50_16585"
     CDS             complement(5319..6449)
                     /locus_tag="VGP50_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007002558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV8029053.1"
                     /translation="MQPGPKDLPRLTDTYFLRTKEIVRRFGDCQVTYAVFMRRPVICT
                     PKLMADFLETMAAERRTTFAIELNYAEGEWVGAGEPMIYLSGSLLHLVDLETVYLQKL
                     GSPCVAAYNAYAMCVDLPKTAFLAMDARHCAGAEMGEMMAYAASVGSAAARRQVGAVG
                     FVGNATDATAPFFGNEHGRGTMPHALIGYAGSTLRAAEMFHESFPAEALTVLVDYFGL
                     EVTDSLAVCRRFPELAAAGRLSLRLDTHGGRYIEELDLARSYDVLERHAPQATRGYRT
                     EAELRYLVGTGVSAAAVWHLRRRLDDAGFTKVKLVASSGFTPAKCRMMAVAGAPIDVV
                     GTGSYLPEIWTETYATADIVAYDGEPRVKKGREFLLRRRGGD"
BASE COUNT         1094 a         2171 c         2166 g         1092 t
ORIGIN      
        1 cgatgcgagc agacgctctg cgcaatctcg ttctcgtgat ggggaaagat aaggtcgagg
       61 ccgccgccat ggatgtcgaa ggtctcgccg agataggtct cgctcatcgc cgagcattcg
      121 atgtgccagc ccggccggcc gcgaccccac gggctctccc agcccggctg atcgggcgtc
      181 gacggcttcc acaggacgaa atcggcgggg tcgcgcttgt agggcgcgat ctcgacccgg
      241 gcgccggcga tcatctcgtc gtgcgagcgg cgcgagagct tgccatagtc cggcatgctg
      301 gacaccgcga acagcacatg tccttcggcg acataagcat ggcccacggc gatcaggcgc
      361 tcgatcatcg cgatcatcgg cgcgagatgc tcggtagcgc gcggctccac atcgggcttg
      421 agcgcgccca gggccgcagc atcgtcctcg aaggttcgag tcgtgcgctg cgtcaacgtc
      481 tcgatgcgtt cgtcattctc cttggcggcg gcgatgatct tgtcatcgat gtccgtgatg
      541 ttgcgcacgt agcgcacatg cttggcaccg tagaggtgcc tcagcaaccg gtagagcacg
      601 tcgaagacga tcaccggccg cgcgttgccg atatgcgcga agtcgtaaac cgtcgggccg
      661 cagacataca ttcggacgtt cgcagcgtcc aacggcgcga acggttcctt gcggcgggtc
      721 agcgtgttgt aaagggcgag cggcacggcg gatcgttgat ggactgcatt agagggagcg
      781 cgggaggggg tagcagattc cgcgcccctg gtccatgagg acccccatga ctccgcgagc
      841 ttgcggccta gtcctcatgc gccggcggag cgccgtagaa gaagcccatc ggccgtccgc
      901 gcggccgcgt ttgcgccgcg agctcggctt cctcgtagcg gcgcttgttc tctctcaccc
      961 cggtcgcggc ctcgatgaaa tagtccgtcg cgccccgggc cgccgcgccg gtcgagcggc
     1021 caaaggtgta aagccccttg gcgtcgtcga cgagccgctt catgtccgcc ccggtgaacc
     1081 cgtctgttgc cgcaagaatg cggtcgacat cgcactcggc aacctcccgc gggacgccac
     1141 ggagatggtt ttcgagaatc cgctgccgcg cctcgcgatc cggcaacttc atctcgagcc
     1201 agagctccac ccgaccgctc cgcaccagcg ccggcggcag atgccgcagg ttcatcgccg
     1261 tcatcatgac gcagacacgc gacgagcctt cgctctcgag gccgtcgagc atcgtcaaca
     1321 gatagcggta gagtccccgt tcctcgccgt cttcgaagat cgcatccgcg tcatcgatga
     1381 agatcacaga cggcgagttg tctttggccg cttcgaagac gcggtggatg cgcgcgtaaa
     1441 agtcgttggt tccggcgatg caggtgccgt cggatcagga cgaatttgcc gcggagccga
     1501 tgcgcgagcg cgcggccgat cgtcgtcttg cccgagccgg gcggtccgta gagcaggacg
     1561 ccgcgcttcg gccggaggcc gaactcggcg gtgagggagt cgttgttgag cggcagcgcg
     1621 acatggatct cgaggctgcg caccacatcg tccaccccaa ggaggtcatg caggtcgacc
     1681 tcctgcactt ccgtcagatc gacattgctg gcaagccgct gcgagcggag atactggatg
     1741 atgccgtcgg tcgaaagcga gcccgatgtc cgcagccagt ctgcggccgc gcgaagctgg
     1801 tgggcgttga gctttggcgc gaagcgatag atcttttgcg cgtcgattgc cgcggcctgc
     1861 tcgtcaccga ccaggtttgc caggagagcc gcgtaatctc ctgccgaata gcggcggatc
     1921 gacgccgaga aggatcgcgt cgcgaaggct tgggcgatcg agccgtccgt cgacacgatc
     1981 gggcaatcgc catctcgccc ggggtctccc aaatatggtg cgcgcatccg gagtattgaa
     2041 tggctgcggt cttgcgattc ttgccgcctg gctgcgcggc tgtgtccggg ccgtatgctc
     2101 gaacccttcc ggctgatcac gcctcgatcg gatcttccgg cacgttcgtc tcaggcgact
     2161 ttcaccgaca gctcggttcc gcccttgagc ggcagggaca tggaccggaa gccgttggcg
     2221 ggatcgcgga cataggccag gtagtccgct tcgccattgt cgttcatcac caggccgccg
     2281 aggcggatct gcggcgcgac gatctccatg acctggcgcg cgagcgacgg tccctcgccc
     2341 cccggccagc catcgacgag catgaaatcg atcggcccgc cgaggacgcg cagcgtttcc
     2401 cgcgcatcgc cttgccgcaa atcgaccaat ccggcgaggc cggcttctgc caagttgcgc
     2461 cgggcggcgg cgactttctc cgggaccagc tcggagccga tcacgacgcc accgccgttg
     2521 tcgcgcacag ccgtcgcgaa atagagcgtc gacatgccga ccgatgtcgc aaattcagcg
     2581 acgcgcgtgg cgccctgcgc ccggcagagg agatagatca gctcgccctg ctcgggatgg
     2641 atcgagaatc cgtaatcggc ataggcgtgc ggatcctgct cgttatcggg attgccgagc
     2701 gggccgccat cggccggacg gcgccgcgcc gcctgcagac gggagatcac cgcgttgacc
     2761 ttcgggtcga ggagcggtcc ggtgggttgg gacatggaac agctttcttg aaggggggat
     2821 atatctgcta cttgccgagg atgtccgcgg cggcgcgccg cagaatctcg gcgatgcgct
     2881 tggtttcctg cggcgagccg tcgctctttt cgcgcaacgc ccgcttcagc gcccggcgtg
     2941 cggcctggag ctcagcggag ccgcgtggac cgagaccgtc atcctccgcg tcgcccgcag
     3001 tttcttctcc ggcaaagacc cggcggacat gctccatttt gccgccgatg cgctcgaact
     3061 cggccagcat cgcatcgacc aggctgcggt tctgttcgag atgcgcgcgg ccggcgtcgg
     3121 tcacgcgata gagcttccgc gtgctctcgg tttcgacagt cgcgtagccg atctcttcaa
     3181 gaaaggtcag cgccgggtag atcacgccgg ggctcgggct gtagaagccg ccggagcgct
     3241 cctccagcgc cttgatcagc tcatagccat ggctcggtct ttccaggagc aaggcgagaa
     3301 tgatgagctg caaatccgcc gaggtgagct tccgcccggt gcggaagcca gcgccgccgc
     3361 gaccgccgcc catgaagccc gctgcgaaat ggccgaagcc ccgcccgccg cgatgccgcg
     3421 ccatgaagcg gaccaggggg ccggtgcggc ggtagtcttg accgatatcg taaaacatgt
     3481 cttacgatat aacgccgccg gctgccgcgc aagcgccaaa ttggcgtcgc cgcgaagcgg
     3541 acggcgacgg tcacgtcgcc gagggcgccg gcgcccgcgg cgtctcgccc ggctcctgta
     3601 cctggcccgg cgccggtgcc ttgtcctgga aggcgttggt gacgggatag cgccggtcgc
     3661 ggccgaaggc gcgccgcgtg agcttgacgc ccggcggcgc ctggcggcgc ttgtactccg
     3721 cgcggtcgag catgcgccac accatgcgca ccgtcgcctc gtcatggccg cgggcaacga
     3781 cctccggcac ggccagctcc ttctcgacca ggcattcgag gatggcgtcc agcacctcgt
     3841 aaggcggcag cgtgtcctga tcggtctggt tggccttcaa ttcggcgctg ggcggcttgg
     3901 tgaggacgcg ctccggaatg accggcccgg cgggacccag caggttgtcc ggcttcgtcc
     3961 ggttgcgcca gcccgacagc gcgaagacgg tcatcttgta gacgtcctta agcacggcgt
     4021 agccgccgca catgtcgcca tagagcgtcg catagccgac ggacatctcc gacttgttgc
     4081 cggtcgacag caccatccag ccgaacttgt tcgacagcgc catcaaagtc atgccgcgcg
     4141 cccgcgactg gatgttctcc tcggtcacgt ccttctcacg cccttcgaag gtgaggcgca
     4201 acatcgcctc gaaggcctgc atcgccggct cgatgctgat cgtccagagg tcgacgccca
     4261 ggagctgcgc gacctcggcg gcgtcatcca cgctctctgc cgaggtaaag ggcgacggca
     4321 tcatcacgca atgcacctgc tctggcccga gcgcgtcgac cgcgacggcg gcggtgaggg
     4381 ccgaatcgat gccgcccgac agccccagca ccacgcccgg gaaccggttc ttgcgcacat
     4441 agtcgcgcaa gcccagcacc atcgcctgat aggtcgcttc gaggccctcg ctcggcgaga
     4501 ccagctttcc cgattcgacg gtccagcggt cgtcggcgcc gcgccgccag accgtcggca
     4561 ccacggtctc cagccaattg ggcgcctgga cgcagagctc gcccgcgaca tcgagcgcga
     4621 aggaggcgcc gtcgaacacc agctcgtcct ggcccgagac ctgattgaca tagagcagcg
     4681 gcaacccgct ctcggtcacc cgctgatggg caagggtgag gcgctcatcg accttgtcgc
     4741 tttcataggg cgagccgttg atgacgatga gcagttgcgc gccgctctcc tggagcgcct
     4801 cggtggcgtc aggctgccac atgtcctcgc agatcatcag gccgagccgc accccatgga
     4861 acgagatcgg ccccggcgcg ggcccggccg cgaagacgcg cttctcatcg aacacgccgt
     4921 aattgggcag ctcgtgcttg aagcgcagcg cgctgatcca gccattctcg agcagagccg
     4981 cagcgttata gagcttgccg tcctggcgcc agggcgtgcc gaggatgacc gcgggaccgc
     5041 gggtcgtcgc cttggcgaat tcgcgcaccg ccgcctggca ggccgcgacg aaggccggct
     5101 tgagcaccag gtcctcgggc ggatatcccg cgacataaag ctccgaggcc accaccagat
     5161 cggcgccgag ttgcgccgcc tcgtccatcg cctggcgcag cttggcgaga ttgcccgcga
     5221 tgtcgcccac ggtcggatcg agctgggcga tggcgatggt caggctgtcg ggggacgcga
     5281 tcatgtctct attatgctaa gaatgagcat aagtgatgtc aatccccgcc gcgccgccgc
     5341 aggaggaatt cgcggccctt cttgacgcgc ggttcgccgt catacgcgac gatgtccgcc
     5401 gtcgcatagg tctcggtcca gatctccggc agataggagc ccgtgccgac gacgtcgatc
     5461 ggcgcgccgg ccaccgccat catgcggcac ttggccgggg tgaagccgga gctcgcgacg
     5521 agcttgacct tggtgaagcc cgcatcatcg aggcgccggc gcagatgcca gaccgcggcc
     5581 gcggagacgc cggtgccgac gagatagcgc agctccgcct ccgtgcgata gccgcgcgtt
     5641 gcctgcggcg cgtgacgctc gagcacgtca tacgagcggg cgagatcgag ctcctcgata
     5701 tagcgcccgc catgggtgtc gaggcgaagc gacagccggc cggccgcggc gagttcggga
     5761 aaccggcggc acaccgcgag cgaatccgtc acttcgagac cgaagtagtc gacgagcacg
     5821 gtcagggcct cggcggggaa gctttcgtgg aacatctcgg ccgcgcgcag cgtcgagccg
     5881 gcatagccga tgagcgcatg cggcatggtt ccgcggccat gctcgttgcc gaagaacggc
     5941 gcggtcgcat ccgtggcatt gccgacaaag ccgacggcgc cgacctggcg ccgcgccgcc
     6001 gccgagccga cggaagcggc ataggccatc atttccccca tctccgcgcc ggcgcagtgg
     6061 cgcgcatcca tggccaggaa tgcggtcttg ggcaggtcga cgcacatggc ataggcgttg
     6121 taagcggcaa cgcaaggcga cccgagcttc tgcagataga cggtctcgag gtcgacgaga
     6181 tggagcagcg aaccgctgag ataaatcatc ggctcgccgg caccgaccca ctcgccctcg
     6241 gcatagttga gctcgatggc gaatgtcgtc cgccgctccg cggccatcgt ctcgagaaag
     6301 tcggccatca gcttgggcgt gcagatgacc ggacggcgca tgaagaccgc ataggtgacc
     6361 tggcaatcac cgaaacgccg cacgatctcc ttggtgcgga gaaagtaggt gtcggtaagt
     6421 cgcggcagat ccttcggccc gggctgcatg attgtcttcg gcggaattat ggatcgcgct
     6481 catgaatccc ctctcccctt gtgggagagg gagggaccca ccg
//
LOCUS       DASYNS010000539        11400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_445913, whole genome shotgun sequence.
ACCESSION   DASYNS010000539 DASYNS010000000
VERSION     DASYNS010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11400
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445913"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VGP50_16590"
     CDS             complement(<1..213)
                     /locus_tag="VGP50_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8029054.1"
                     /translation="MTDPASIVYIIDDDASVREGIADLLASVGLATQSFGSTQEFLKI
                     ERADAPGCIVLDVRLPGSSGLEFQRGL"
     gene            complement(210..1289)
                     /locus_tag="VGP50_16595"
     CDS             complement(210..1289)
                     /locus_tag="VGP50_16595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8029055.1"
                     /translation="MDSIRNSRGPYLLAKWLGGYVGIVALGYACYRFGFDFATSGFAL
                     LIAIVLMSMLGGYIGSITGSIIAYVCLNYFVLPPVLSFEVETPEDILSAFAFLITSIV
                     VTGLTSKVRRAREEELRETRAELARFARVAVLGEMTASIAHEINQPLAGVVSSGNACQ
                     HWLSAQPPNIERAIESLNRIIRDANRAAEVVGRVRSLIKKSPPKAGPIDINEAVREVT
                     ILTRGEMARQRVVLTTELAGGLPPVRADRIQLQQVCLNLIVNAIEAISARAGGPRGLS
                     ISTGLDDAGWVKLTVRDTGIGIEPGKLETIFDAFHTTKRDGIGLGLAISRSIVEAHGG
                     RLWAMPDQPRGATFQLALPTVGHAE"
     gene            complement(1479..2690)
                     /locus_tag="VGP50_16600"
     CDS             complement(1479..2690)
                     /locus_tag="VGP50_16600"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8029056.1"
                     /translation="MKAKPWMRLLLAIAAVLLIAAPALAQKKYAPGVSDTEIRIGQTM
                     PYSGPASAYAAIGRAEAAYLTMLNEQGGVNGRRITLLSLDDGYSPPKTFEQTRRLVEQ
                     ENVAFIFSSLGTPTGVVVHKYLNEKNVPQLFQSSGATFWGDPEHFPWSMGWQPNYQTE
                     GAIYGRYLLRVKPNARVAVLYQNDDAGRDYVAGFKAGLGTEAAQRMIIAVATYETTDP
                     TLESQIVALQASGADVLFDNSTPKFAAQAIRKVYDVGWHPLHFLTSVASSVSAVLEPA
                     GFDKSQGLVSAAYLKDPNDPQWKDDPAYKDWLAFMQRYDPEGNLGDSFNVSGYALAQT
                     VVQVLKQCGDDLSRENIMRQAANLDLELPMLLPGIKVRTSPNNFYPVQRMRLARFEGK
                     QWTLFGEVLGE"
     gene            3032..4414
                     /locus_tag="VGP50_16605"
     CDS             3032..4414
                     /locus_tag="VGP50_16605"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEV8029057.1"
                     /translation="MTVATSVRPAAVETAVTAEIARRAAGLRHGDLPDDIRLLARQCL
                     LDWLGVTLAGAGEELSRILCTEAEEQGGNGAATLIGHGRKVPTQQAALVNGAASHALD
                     YDDVNMTMGGHPTVPVVPALLALAEARQASGAQFVTAFVAGYEALCRVGALVAPGHYA
                     QGFHATATVGSFGAAAACAHLLGLDAPATAVALGIAGTQAAGLKSMFGTMCKPLHAGK
                     AAQNGLLAASLAARGFTSRSDVLDCAQGFARTQSSDFHPQAALAEPPGGWHLRHNLFK
                     YHAACYLTHAPIECAREIRERLALDPGAVSEAVLRVDAGASKVCHILAPRTGLEAKFS
                     LRLTTAFALAGRDTARLDTYDARNAADPVLARLRDKVRVDFQRHWPHTLAELTVTMAD
                     GRVAEARHDSGKPATDLAAQGRRLESKFMALAAPLLGDAQAERLAAAVAALDSLGSMA
                     ELTRLCAGCA"
     gene            4489..5283
                     /locus_tag="VGP50_16610"
     CDS             4489..5283
                     /locus_tag="VGP50_16610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019086628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8029058.1"
                     /translation="MDLELRDRRALVTASSDGIGFAIAKGLAAEGASVILNGRTADGV
                     AAALARLRETLPKASVEGVAADAATASGAETIFARVPAIDILVNNLGIYEQKPFFDIP
                     DSDWMRFFETNVLSGIRMARRYAPAMREQNWGRILFVSSESALNIPREMIQYGMTKTA
                     QLAISRGLAIELAGSGVTVNALLPGPTLSRGVKTFLDRAVKASGKPLAEIEAGFFRTA
                     RPSSLTRRFAEVEEVANLAVYLSSPRSSATTGAALRVDGGIVNSIG"
     gene            5283..6140
                     /locus_tag="VGP50_16615"
     CDS             5283..6140
                     /locus_tag="VGP50_16615"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEV8029059.1"
                     /translation="MADPARLRATRSVFLAGVLVAAAVLFAVPAAAQTPTALTNWQLS
                     VGELLVKQAEPVPEWRVTLGGGIDFDPIYEGAKRYRTLPSLVFDIRYQDLFFVSDGEG
                     LGVNILRGQNYRAGVAIGYDLGRDHHLQHRLAGLGNVDPTAEAKIFAEYFFKAVVLNV
                     DLRKGIGGNSGLVGDVGFYVPLQVPFIENFYVFTGPSVTLADHEYMDSYFSVTSEQSA
                     RSIFPVFNAHAGLYKSGWGVTAIYDLSDNWWLEAEGAWQYLLGGAAASPIVEDRSEFN
                     AGVNVIYRF"
     gene            complement(6272..7711)
                     /locus_tag="VGP50_16620"
     CDS             complement(6272..7711)
                     /locus_tag="VGP50_16620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8029060.1"
                     /translation="MPEDGRPLLPAPGGAAAQAIDPRRWWVLWTVAAAQFMFVVDAFI
                     VNVAIPTIRADLRIGAAEIEAVIAVYLIAYAALVIVGGRLGDLHGVKPVFLSGLAGFV
                     MASVWCGLARSGVELIAARLAQGAMAALMVPQVLATIHVLFPDGERARAFAVYGFALG
                     FGGAAGFLLGGSLVTLDLGGLSWRLVFFVNAPLGLAIAAMAWRLIPRVKRRSRAPLDL
                     AGAAVLFAGLLCLIAPLLFGRDFHWAPWLWLVMLLGLLVIAGFLALERHVERSGGLPL
                     VASALIADRLFLHGLAAAFCFFSANLSFYFVLTLFLQGGLGYSPWEAGLTVLPLALAF
                     VIASRRGMARGAARGGLGPVAGCGWQIIGLGALALVADFSAQPAMLLLALTLAVFGYG
                     QGLVMAPLSSIVLATVRHADAGSASGVYATTVQIANAAGVAAIGALFFTAQETLSDRL
                     AFLAALAAVAAMVAAAASLLAWRRHAVRV"
     gene            complement(7704..8456)
                     /locus_tag="VGP50_16625"
     CDS             complement(7704..8456)
                     /locus_tag="VGP50_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029061.1"
                     /translation="MSTVTDLIERYVAVWNEANPAERRRRIRALWAPDGGTCHRLHDA
                     QGYDAIEARVAGSWDKLLRAGNHVFRPRKAVGHHDVVKFDWVMATVPEGTIAAAGLSF
                     LVLAPDGRITADYQFNPAVNEAGPLVERYLAVWNESDAARRRRAVAELWAPDGVHVSA
                     NAVRNGRAAIEAEAAEIGGAAAAQGVVFEAADRSHAHHDVVSFDWQRRRTKDRTVTAV
                     GSELLILDAAGQIRFDHRYEDSLVAAAGGVHA"
     gene            complement(8513..9292)
                     /locus_tag="VGP50_16630"
     CDS             complement(8513..9292)
                     /locus_tag="VGP50_16630"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV8029062.1"
                     /translation="MTQAVYRLAGQCAHASASAAGPWNPSLQHGAAPAALVAWAAERI
                     ATDMPMNVARLTVDLLRPVPVAPLEIRSAVVRQGRKIQVASILLLANGVEVVRASVLK
                     IRARDAALPDSVSAPPLDLPAPELGHEPGAGWRIKSAFLEGISIRLADGPVGRPGRAA
                     IWFRAERPIVEGEPISPLMRAALTADFCNGISTLLDASQWSFINGDVSINLTRPPVGE
                     WILLDAETWLERDGGGIAFARMGDRAGYFGRAVQSLVIERR"
     gene            complement(9289..10191)
                     /locus_tag="VGP50_16635"
     CDS             complement(9289..10191)
                     /locus_tag="VGP50_16635"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV8029063.1"
                     /translation="MGRLQDKVAVITGGTSGIGARTAQLFVAEGAKVVIAGRRDDEGQ
                     TLAAGLGAAASFIRTDVTREADMRSMIAHALERFGRIDCLFNNAGGPSRTGGVAEVDI
                     AEFDAAIAVHVRGALLGMKYAAPGMLRQGSGSIINMASINGRRAGFTTLGYSTAKAAV
                     IHLSRCAAVELGQHGVRVNSLSPGPVLTGIFGKAAGRDHGEADREAETMKAAFDRLLP
                     AVQPIPRVGMPDDIARAALYLASDDSSLVNGHDLVVDGGITAGRPASVMRGERAVFAE
                     VLGAGGPQRSAIAPSHTSDPRESR"
     gene            10394..11224
                     /locus_tag="VGP50_16640"
     CDS             10394..11224
                     /locus_tag="VGP50_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014199358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8029064.1"
                     /translation="MQQPPFGVVLRRWRERRRMTQMDLAFAANASTRHLSYLETGRAQ
                     PSREMVMRLGEHLDVPLRERNTLLLTAGFAPAFQERPLAELSAAREAIAQVLEAHKPY
                     PAFAVDRHWNIVLSNRVLPQLYVDVAPELLRPPVNSVRLTLHPLGMAPRIANLGEWRN
                     HVIAELRHQLDARADAGTQALLAEVMGYPAPPGSAPGEREPGAERYATPLRIRTEAGV
                     VSFLSTITVFGTPTDVTLAELALEMLFPADADTVAIIGAMTRAESGAAPRNAALADAD
                     "
BASE COUNT         1886 a         3865 c         3883 g         1766 t
ORIGIN      
        1 gagcccgcgc tggaattcga gcccgctcga tcccggcagg cgcacgtcga ggacgatgca
       61 gcctggcgcg tccgcgcgct cgatcttgag gaactcctgg gtcgaaccga aggattgcgt
      121 cgcgagcccg accgaggcga gaagatcggc gatgccctcg cgcacggaag cgtcgtcgtc
      181 gatgatatag acgatggatg ccggatcggt cattccgcgt gccctaccgt cggcaaggcg
      241 agttgaaagg tcgcgccgcg cggctgatcg ggcatggccc acagccgccc gccatgcgcc
      301 tcgacgatcg agcgactgat cgcgaggccg aggccgattc cgtcgcgctt ggtcgtgtgg
      361 aacgcgtcga agatcgtctc aagcttgccg ggttcgatgc cgatgccggt gtcgcgcacc
      421 gtcagcttca cccaaccggc gtcatcgagc ccggtgctga tcgacagacc gcgcggacca
      481 cccgcgcggg cgctgattgc ttcgatcgcg ttgacgatca ggttgaggca gacctgctgc
      541 aattggatgc ggtcggcccg caccggcggc aggccaccgg cgagctcggt ggtcagcacc
      601 acgcgctgtc gcgccatttc gccgcgcgtg agaatggtca cctcgcggac ggcctcgttg
      661 atgtcgatcg gccccgcttt cggcggcgat ttcttgatca ggctgcgtac gcggcccacc
      721 acctcggcag cccggttggc gtcgcggatg atccggttga gcgactcgat ggcgcgttcg
      781 atgttgggcg gctgggccga cagccagtgc tggcaggcgt tgccgctgct gaccacgccg
      841 gccagcggct ggttgatctc atgcgcgatc gaggccgtca tctcacccag cacggcgacc
      901 cgcgcgaagc gggccagctc ggcgcgcgtc tcgcggagct cttcctcgcg cgcgcgccgc
      961 accttgctcg tcagccccgt cacgacgatc gaggtgatga ggaaggcaaa tgccgagagg
     1021 atgtcttccg gagtctccac ctcgaaactc aacaccggcg gcaatacgaa gtaattcaag
     1081 caaacatacg cgatgatcga gccggtgatc gagccgatgt agccgccgag catcgacatc
     1141 aacacgatcg cgatcaggag agcgaaaccg gacgtggcga aatcgaaacc aaaccggtag
     1201 caggcatagc ccagcgccac gatgccgaca tagccgccca gccacttggc gagcagatac
     1261 gggccgcggg agttccgaat gctgtccacc agatgctcgg ccgttcgcag aatcgttgaa
     1321 gatcgccgtc tcgccgtggt tctgatcatg cgcagatatg cgcatatctg cgcatatcct
     1381 tggggcagtt aacacgagtt gatccgatgt gcattgcgat ggatcaagtc actcaagagt
     1441 cagcgcaaga ttcgccggct ggtgccgcgc cgcttctctc actcgccgag cacctcgccg
     1501 aagagcgtcc attgcttgcc ttcgaaacgg gcgaggcgca tgcgctgcac ggggtagaaa
     1561 ttgttggggc tggtgcgcac cttgatgccc ggcagcagca tgggaagctc gagatcgagg
     1621 ttggccgcct ggcgcatgat gttctcgcgc gagaggtcgt cgccgcactg cttcaggacc
     1681 tgcaccacgg tctgggcgag cgcgtaaccg ctgacgttga agctgtcgcc gagattgcct
     1741 tcgggatcgt atcgctgcat gaaggcgagc cagtccttgt aggccgggtc gtccttccat
     1801 tgcgggtcgt tcgggtcctt gagataggcc gcgctgacga ggccttgcga cttgtcgaag
     1861 ccggcgggct cgagcacggc cgagaccgag ctcgccaccg acgtgaggaa atgcagggga
     1921 tgccagccga cgtcgtagac tttacggatc gcttgcgcgg cgaatttcgg cgtcgagttg
     1981 tcgaacagca cgtcggcgcc ggaggcctgg agtgccacga tctgcgattc gagagtcgga
     2041 tcggtcgtct cataggtggc gacggcgatg atcatgcgct gtgccgcctc ggttccgagc
     2101 ccggccttga accccgcgac atagtcccgg cccgcgtcgt cgttctggta aagcacggcg
     2161 acccgggcgt tcggcttcac ccttaagagg tagcggccat aaatggcgcc ttcggtctga
     2221 taattgggct gccagcccat gctccaggga aaatgctccg gatcgcccca gaaggttgcg
     2281 cccgacgatt gaaaaagctg cggcacattc ttctcgttga gatatttgtg gacgaccacg
     2341 cccgtcggcg tgccgaggct cgagaagatg aaggcgacat tctcctgctc gaccagccgg
     2401 cgcgtctgct cgaaagtctt gggcgggctg tagccgtcat cgaggctcaa aagggtgatg
     2461 cggcggccgt tcacgccgcc ctgctcgttg agcatggtga gataggccgc ctcggcacga
     2521 ccgatggccg cataagcaga ggccggcccg ctatagggca tggtctgccc gatccggatc
     2581 tccgtgtcgc tgacaccggg cgcgtatttc ttctgcgcca gcgccggagc ggcgatcagg
     2641 agcactgccg caatcgccaa aagcagccgc atccaaggct tcgccttcat tgtcgtcctc
     2701 ccttgttctt ttctggagat atggtccggc ttttgccgag ttctggcaag cgggaccagc
     2761 gggtgctccg acaaagaccg agccgtgctc cgacaaagac aaagaggaac gcggagggcg
     2821 ccgaggttgc gctgaggacg cagaggaatg gtacctcggc ggcctcggcg caaccctctg
     2881 cgccctccgc gttcgctgat ggaccgaagc gccgtgccgg cgcgcctggg ggcgacgatc
     2941 ttggaaatgt cgattctact actccgtgat cggggctgga cgcgtgccgc ctgggcgtgc
     3001 cagcttcggg taaagatcga ggggagggaa catgaccgtg gcaacgtccg ttcgtccggc
     3061 ggctgtcgag acggccgtta cggcggagat cgcgcggcgc gccgcgggtt tgcgccatgg
     3121 cgacctgccg gacgacatcc gcctcttggc gcgccaatgc ctcctcgact ggctcggcgt
     3181 gacgctggcg ggcgccggcg aggagctgtc gcgcatcctt tgtacggaag cggaagagca
     3241 agggggcaac ggcgcggcaa cgctgatcgg ccatggccgc aaggtgccga cccagcaggc
     3301 ggcgctcgtc aacggtgccg cctcccatgc cctcgactat gacgacgtca atatgacgat
     3361 gggcggccat ccgacggtgc cggtggtgcc ggcactgctc gcgctggccg aggcacgcca
     3421 ggcgagcggc gcgcaattcg tgaccgcctt cgtcgccggc tacgaagcgc tctgccgcgt
     3481 cggcgccttg gtggcgccgg gccactacgc gcaaggcttc catgcgaccg cgacggtggg
     3541 aagcttcggc gcggcggccg cctgcgcgca tctgctcggc ctcgatgcac cggctacggc
     3601 cgtggcgctg ggcatcgccg gcacgcaagc cgccggcctc aaatcgatgt tcggcaccat
     3661 gtgcaagccg ctccatgccg ggaaggctgc gcagaacggg ctcctcgccg cgagcctcgc
     3721 tgcccgcggc ttcaccagcc ggagcgacgt gctcgattgc gcgcagggct tcgcgcgcac
     3781 gcaaagttcc gatttccacc cccaagccgc gctcgcggag ccgcccggcg gctggcacct
     3841 gcgccacaac ctcttcaagt atcacgcggc ctgctacctc acccacgcgc ccatcgaatg
     3901 cgcacgcgag atcagagaga ggctcgcgct cgatccgggc gcggtcagcg aagccgtgct
     3961 gcgtgtcgac gccggtgcga gcaaagtgtg tcatatcctc gcccccagga ccgggctcga
     4021 agccaagttc tcgctgcgcc tcaccaccgc tttcgcgctt gccggccgtg atacggcgcg
     4081 gctcgacacc tatgacgcgc gcaatgccgc cgatcccgtg ctcgcgcgat tgcgcgacaa
     4141 ggtccgcgtc gatttccagc gccactggcc gcacaccctg gcggagctga cggtgacgat
     4201 ggccgacggc cgcgtcgccg aggcgcgcca cgattccggc aagcccgcca ccgacctcgc
     4261 ggcgcaaggc cgccgcctcg agagcaagtt catggcgctg gcggcaccgc tgctgggcga
     4321 cgctcaggca gagcgcctcg ccgcggcggt cgcggcgctc gacagcctcg gctcgatggc
     4381 cgagttgacc cggctctgcg cgggatgcgc atgacgatcg ctgcgcgggc gatgctttgc
     4441 gttgcgcggt gtttgtgctc gacaaatgga attcaggtaa ggggaaacat ggatctcgaa
     4501 cttcgcgacc gccgggcgct ggtcaccgcg tcgagcgacg gcatcggctt tgccatcgcc
     4561 aaaggccttg ccgccgaggg cgcttcggtc attctcaacg gccgcacggc agacggcgtc
     4621 gcggccgcgc tcgcgcggct gcgcgagacg ctgcccaagg cgagcgtcga aggcgtcgcg
     4681 gccgacgccg cgaccgcttc cggcgccgaa accatcttcg cgcgcgttcc cgccatcgac
     4741 atcctcgtca acaatctggg gatttacgaa cagaagccgt tcttcgacat tcccgacagc
     4801 gactggatgc gcttcttcga gacgaatgtg ctgagcggca tccgcatggc gcgccgctac
     4861 gcgcccgcca tgcgcgagca gaattggggg cggatcctct tcgtctcgag cgagagcgcg
     4921 ctcaacatcc cgcgcgagat gatccagtac ggcatgacca agacggcgca gctcgccatc
     4981 tcgcgcggcc tcgccatcga gctcgccggc agcggcgtca ccgtgaatgc gctgctgccc
     5041 ggcccgacgc tgagccgcgg cgtcaagacc ttcctcgatc gcgccgtgaa ggcgtcgggc
     5101 aagccgctgg cggagatcga ggccggcttc ttccggacgg cccggccctc gtcgctgacg
     5161 cggcgcttcg ccgaggtcga ggaagtcgcc aatctcgcgg tctatctctc gagcccgcgc
     5221 tcctcggcga ccaccggagc ggcgctgcgc gtcgacggcg gtatcgtcaa cagcatcggc
     5281 tgatggcaga tccggcgcgc ctgcgcgcga cgcgcagcgt tttcctggcg ggcgttctgg
     5341 tggcggcggc ggtgctcttc gccgtgccgg cggcggcgca gacgccgacg gcgctcacca
     5401 actggcagtt atcggtcggc gagctgctgg tgaagcaggc ggagccggtg ccggaatggc
     5461 gcgtgacgct gggcggcggc atcgatttcg atccgatcta cgaaggcgcg aagcgctacc
     5521 ggaccctgcc ctcgctcgtt ttcgacatcc gctaccagga tctcttcttc gtgtcggacg
     5581 gagaagggct cggcgtgaat atcctgcgcg gccagaacta ccgcgccggc gttgccatcg
     5641 gctacgacct cggccgcgat caccatctcc agcaccgcct cgccggtctc ggcaacgtcg
     5701 atccgaccgc cgaggcgaag atcttcgccg aatacttctt caaagccgtc gtcctcaacg
     5761 tcgatctgcg caagggcatc gggggcaaca gcggcctcgt cggcgatgtc ggcttctacg
     5821 tgccgctgca ggtgccgttc atcgagaatt tctatgtgtt caccggccct tccgtgaccc
     5881 tcgccgacca tgaatacatg gactcctatt tctccgtcac gtcggagcag tcggcgcgct
     5941 cgatcttccc cgtcttcaac gcgcatgccg gcctctacaa gtccggctgg ggagtgaccg
     6001 cgatctatga cctgagcgac aattggtggc tcgaggcgga aggagcgtgg caatatctgc
     6061 tcggcggtgc cgcggccagc ccgatcgtcg aggaccgcag cgaattcaac gccggcgtca
     6121 acgtgatcta tcggttttag caaagagaca agaaagacga acgcggaggg cgcagaggct
     6181 ccgcggagga cgcaaaggaa tagccatgct ctgcggcctc aacgcaacct ccgcgccctc
     6241 tgcgttcgcg atgaaggagg atgcggctcc gtcacacgcg cacagcgtgc ctccgccaag
     6301 ccaagaggga cgcagcggcg gcgaccatcg cggcgacggc ggcgagcgcc gcgaggaagg
     6361 cgaggcggtc ggaaagagtc tcctgcgccg tgaagaacaa cgcgccgatg gcggcgacgc
     6421 cggcggcgtt ggcaatctgc accgtcgtcg cgtagacgcc cgatgccgag ccggcatcgg
     6481 cgtgccgcac ggtcgcgagc acgatgctgg agagcggcgc catcaccagc ccttgtccgt
     6541 agccgaacac cgccagagtc agcgccagaa gcagcatggc cggctgcgcc gagaagtcgg
     6601 cgaccagcgc aagcgcgcca agcccgatga tctgccagcc acagccggcg acggggccaa
     6661 ggccgccgcg cgcggccccc cgggccatgc cgcggcggga cgcgatgacg aatgcgagcg
     6721 ccagcggcag caccgtcagg cccgcctccc agggcgaata gccgaggccg ccctgaagaa
     6781 agagcgtcag cacgaaatag aaggacagat tggccgagaa gaagcagaag gcggcggcca
     6841 ggccgtgcag gaacaagcgg tcggcgatga gcgcagatgc gaccagcggc agcccgccgc
     6901 ttctttcgac gtggcgctcg agggcgagga agccggcgat caccaggagg ccgagcagca
     6961 tcacgagcca gagccacggc gcccagtgaa aatcccggcc gaagagcagc ggggcgatca
     7021 ggcacaagag gccggcgaag agcacggccg ctccggccag atccagaggc gccctcgatc
     7081 gccgcttgac gcgcgggatc agccgccagg ccatggcggc gatcgccagg ccgagcggcg
     7141 cgttgacgaa gaatacgagg cgccagctca ggccgccgag atcgagagtg accaaggatc
     7201 cgccgagcag gaagcccgcg gcgccgccga agccgagcgc gaagccgtag acggcgaagg
     7261 cgcgggcgcg ctcgccgtcg gggaacagga cgtggatggt cgccagcact tgcgggacca
     7321 tcagcgccgc catggcgccc tgcgcgaggc gcgcggcgat cagttcgacg ccggagcggg
     7381 cgaggccgca ccacaccgat gccatgacga agccggcgag ccccgacagg aataccggct
     7441 tcacgccgtg gagatcgccg agccgcccgc cgacgatcac cagcgccgca taggcgatga
     7501 gatagacggc gatgacggcc tcgatctcgg cggcgccgat gcggagatcg gcgcggatcg
     7561 tcgggatcgc cacgttcacg atgaaggcgt cgacgacgaa catgaactgc gccgccgcca
     7621 cggtccacaa cacccaccag cggcgcggat cgatggcttg cgcggcggcg ccgccgggcg
     7681 cgggcaggag agggcggccg tcctcaggca tggacgcctc cggccgccgc gacgagcgag
     7741 tcctcgtagc gatggtcgaa gcggatctgc ccggcggcgt cgagaatcag caactccgag
     7801 ccgaccgccg tgaccgtgcg gtccttcgtc cggcgccgct gccagtcgaa gctcacgacg
     7861 tcgtggtgag cgtgcgaccg gtccgccgct tcgaagacga ctccctgtgc agcagcagcg
     7921 ccgccgattt ccgcagcctc ggcctcgatc gcggcacggc cgttccttac ggcgttcgcg
     7981 ctcacatgca cgccgtcggg tgcccagagc tcggcgacgg cgcggcgacg gcgagcggcg
     8041 tcgctttcgt tccagacggc gagatagcgc tcgacgagag ggccggcctc gttgaccgcg
     8101 ggattgaact ggtaatccgc cgtgatgcgg ccgtccgggg cgaggaccag gaaactcaat
     8161 ccggcggcgg cgatggtgcc ctcgggcacc gtcgccatga cccagtcgaa tttcaccacg
     8221 tcatgatggc cgacggcctt gcgcggccgg aagacgtgat tgccggcgcg cagcaatttg
     8281 tcccacgagc ccgcgacgcg tgcctcgatc gcgtcatagc cctgcgcgtc gtgcagccga
     8341 tggcaggtgc cgccgtccgg cgcccagagc gcgcggatgc gccggcgccg ctcggcggga
     8401 ttggcctcgt tccacacggc cacatagcgc tcgatcaagt ccgttacggt cgacatgacg
     8461 ccatctcctt tcaagatttc cgcgccgcga tcgcgaagcg ggtttcgctg gctcaccttc
     8521 gctcgatgac cagcgactgc accgcgcggc cgaaatagcc ggccctgtcg cccatgcgtg
     8581 cgaaagcgat cccgccgccg tcgcgctcga gccaggtttc ggcatcgagc aggatccatt
     8641 cgccgaccgg cggacgcgtc aggttgatcg acacgtcacc gttgatgaaa ctccactggc
     8701 tggcgtcgag cagggtggat atgccgttgc agaaatcggc cgtcagggcc gcgcgcatca
     8761 gcgggctgat gggctcgccc tcgacgatcg gccgctcggc gcggaaccag atcgccgcgc
     8821 ggccagggcg cccgacgggc ccatcggcga gcctgatcga gatgccctcg aggaaggcgc
     8881 tcttgatgcg ccagcccgcg cccggctcat gccccaactc cggtgccggc agatcgagcg
     8941 gcggcgcgct cacgctgtcg gggagggccg catccctcgc gcgaatcttc agcacgctgg
     9001 cgcgcacgac ctcgacgccg ttcgccagca ggaggatcga ggcgacctgg atctttcgcc
     9061 cctggcgcac gaccgcgctc cgtatctcca gcggcgcaac cggcaccggc cgcagcagat
     9121 ccaccgtcaa ccgcgccaca ttcatcggca tatccgtcgc gatgcgctcc gccgcccagg
     9181 cgacgagcgc ggccggcgcg gcgccgtgct gcagagaggg attccacggc cccgccgcgc
     9241 tggcgctggc atgagcgcac tgaccggcga gccgataaac cgcctgcgtc atcggctctc
     9301 tcgaggatcg ctcgtgtggg acggagcgat ggcactgcgc tgcggcccgc cggccccaag
     9361 cacttccgca aacacggccc gctcgccgcg catgaccgat gccggccggc cggcggtaat
     9421 gccgccatcg accaccaggt catggccgtt gacgaggctc gagtcgtcgc tcgctagata
     9481 gagtgccgcc cgcgcgatat cgtcgggcat gccgacgcgc ggaatcggct gcaccgcggg
     9541 aagcagccgg tcgaaagcgg ccttcatcgt ctcggcctcg cgatccgctt cgccgtggtc
     9601 gcggccggcg gccttgccga aaatcccggt gagaaccggc ccgggggaaa ggctgttcac
     9661 gcgaacgccg tgctggccga gctcgaccgc ggcgcagcgc gacaggtgga tcacggcggc
     9721 tttggcggtc gagtagccga gcgtcgtgaa accggcgcgg cgcccgttga tgctggccat
     9781 gttgatgatg ctgcccgagc cctggcgcag cattccgggc gcggcgtatt tcatcccgag
     9841 gagtgcgcca cggacatgga cggcgatcgc ggcatcgaac tcggcgatgt cgacctccgc
     9901 cacgccgccg gtgcgcgatg gaccgcccgc gttgttgaag agacaatcga tccgcccgaa
     9961 gcgctcgagg gcatgtgcga tcatcgatcg catgtccgcc tcgcgcgtga cgtcggtgcg
    10021 gatgaagctc gccgctgcgc ccaggcccgc ggcgagggtc tggccctcgt cgtcgcgccg
    10081 cccggcgatg acgaccttgg cgccttccgc gacgaagagt tgggcggtcc gcgcgccgat
    10141 cccgctcgtg ccgccggtga tcacggcaac cttgtcctgc agccggccca ttggatgtct
    10201 cctgcgttgc tcgtgccgtg cgggccggta cccttttggg cgcgggacca ccaagcgatg
    10261 ccgcgaagga tggtctcggc acggccggcg gtcgatgacg tcggaggtaa aaacccgtcg
    10321 ccgccggtgt cgcttcttga cctccgaggt catggccgcc ggacgccgtt cgtgctaggc
    10381 aaggcggatg agcatgcaac aaccgccctt cggcgtggtg ctgcgccgct ggcgcgagcg
    10441 ccggcggatg acgcagatgg atctcgcctt cgccgcgaac gcctcgacgc ggcatctgag
    10501 ctatctcgaa accggacgcg cgcagccgag ccgcgagatg gtcatgcgtc tgggcgagca
    10561 tctcgacgtt cccctgcgcg agcggaacac gctgctgctc acggcgggat tcgcgccggc
    10621 gttccaggag cggccgctgg cggagctgag cgccgcgcgc gaggcaatcg cgcaggtcct
    10681 cgaagcgcac aagccctacc cggctttcgc cgtcgaccgg cactggaaca tcgtcctgtc
    10741 gaaccgggtg ctgccgcagc tctatgtcga tgtcgcgccc gagctcttgc ggccgcccgt
    10801 caactcggtc cgcctcacgc ttcatccgtt gggcatggcg ccgcgcatcg ccaacctagg
    10861 ggaatggcgc aaccacgtga tcgccgagct caggcaccag ctcgacgcgc gggcggacgc
    10921 cggcacgcaa gcgctgttgg cggaagtcat gggctacccg gcaccgcccg gatcggcgcc
    10981 gggcgagcgg gagccgggtg cggagcgcta cgccacgccc ttgcggatca ggacggaggc
    11041 gggcgtcgtc tcgttcctga gcacgatcac cgtcttcggc acgcccacgg acgtcacgct
    11101 cgccgagctc gcgctcgaga tgctgtttcc cgccgacgcc gacacggtgg cgatcatcgg
    11161 cgcgatgacg agggcagaga gcggcgctgc tccgcgcaat gcagcgcttg cggatgcgga
    11221 ttgagttgcc ccctctcccg cgatgcgcgt gaggggcgaa gtacggacta atggcactat
    11281 ctggctgtcg ttccctttga cgtcgccctc agcgatcttg ccagcggccg cgcggccggc
    11341 gagcggcatc tgctgcgacc agatcgtcgt cgctctgttg cgggttcgtg gccttggcca
//
LOCUS       DASYNS010000540         8171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3568314, whole genome shotgun sequence.
ACCESSION   DASYNS010000540 DASYNS010000000
VERSION     DASYNS010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3568314"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="VGP50_16645"
     CDS             complement(<1..1373)
                     /locus_tag="VGP50_16645"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV8029065.1"
                     /translation="MPSALRLLRDRPAWVLAVLALLLHLYASGGYGVFRDELYFIVCG
                     SRLDWGYVDQPPLIPLIAWTMHSLFPASLVMLRLVPALAHAGTIALTGETARVLGARV
                     WGQGLAALCVLVAGVYLGMGTLLSTDALQPLAWSFCSYALIRVIRERDERWWLAMGPV
                     VGVALLSKYMIVFWLAALGLGIVATPARRSLARPPLYAGAAIALLIVLPNVLWQWAHD
                     WPFLEIGRRAAEGKNVALSPLAFLAAEIENLNPGTAPVWLIGVAAFAFWRRLADLRLF
                     AVAFIALVAVMIAVHAKPYYPVGAYTVLFAGGATALEAWLSRPAPRAALAAAVALLGV
                     VLAPFALPILPVARFAAYEERLGQSPKPLERLAVGRLPQYYADMFGWSELAALVGRAY
                     QALPAEERTQAVFLARNYGEAAAVDVLGTAWGLPPAISGHNSYFLWGPRGHDGSVVLL
                     LGGEREA"
     gene            1537..1623
                     /locus_tag="VGP50_16650"
     tRNA            1537..1623
                     /locus_tag="VGP50_16650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1571..1573,aa:Leu,seq:taa)
     gene            complement(1691..2350)
                     /locus_tag="VGP50_16655"
     CDS             complement(1691..2350)
                     /locus_tag="VGP50_16655"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8029066.1"
                     /translation="MKRTKSLATCVSDTAPPVPVRDRILRAAFNAFTENGYAETSTLE
                     IATRAKVSKRELYALFGNKKAMLVASIADRVTRMRLPPDLPAARDRETLARILRIFGA
                     ILLREVCHPSVMAVFRLGITEATRSPEVAQVLDAEGSQPNRAALSDVLAQAQEAGLLE
                     AGDPTDMAGEFLALLWRDLMMRLMLRVIKSPDPTEIERRALDATTAFLKLHPKPQPLC
                     T"
     gene            2554..3063
                     /locus_tag="VGP50_16660"
     CDS             2554..3063
                     /locus_tag="VGP50_16660"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV8029067.1"
                     /translation="MTKMNRRIALALASVSVIAAAIAPTPVFAADESAANAALLKRYV
                     AAFNAHDAAGFKDIVAENYIQHNGRAGQGLAGLQAAASQYFQTFPDFHMQLEDSVMNG
                     DKVVARFTLTATHDHDVQLGPGAPTFPPTGKKLSWSGISIWRVADGKFAEHWDVDDLA
                     GLARQMRGQ"
     gene            3114..3635
                     /locus_tag="VGP50_16665"
     CDS             3114..3635
                     /locus_tag="VGP50_16665"
                     /inference="COORDINATES: protein motif:HMM:NF042671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="HEV8029068.1"
                     /translation="MPNVANMLQNMPWWAFVLLALLVVLGVQALQPRMIAVWRLLIVP
                     ATFIGWGVISLVTQSVASPILMADWLATAAAGAAIAWGATRLEGMRIDRPGGHVQIPG
                     SVFPLMRNLVIFAAKYGLTVATTMAPSMQMTLSLWNIAVSGLSAGYFIGWLLRFALKY
                     RTTPEPQTGHPVG"
     gene            3793..5817
                     /locus_tag="VGP50_16670"
     CDS             3793..5817
                     /locus_tag="VGP50_16670"
                     /inference="COORDINATES: protein motif:HMM:NF019874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8029069.1"
                     /translation="MAFLDRCRIKVKLALIVGVCALALVAALAAAAEMQRQRMISDRI
                     DKARAIVEIAYGLADSLEREVVARHLTHDEALARFTDDLRAMWYDNHQNYIVLGSLDN
                     VWMIHPAVPRVEGTIGSTDLENPSLYLITLFTDAVRSVDEAIVRYRYPRPGQKERLLK
                     ITFTKKFTPWNAFIASGAYVDDIEAEYRSVLGRLALIGLLIMTAAAATAYAISRNISH
                     SLTALKGRMEQLAAGDLTVDIREAGRQDEIGEMARTVQVFKDNALEKERMHGELQHKE
                     ELRRTAESLRRSQERLAQAQRLAGMGSDFRDIANDVADWSDETYRIFGVARETFTPTT
                     EGLLSLIHPDDRGLVLAARGEVVRGICPAPFDHRIVRRDGSLRIVRRETELLRDETGT
                     VVSLAGTTHDVTEQRAAQKRQSELERQVLHSQKLDALGTLAGGIAHDLNNTLVPVLGL
                     AKLTMGRMAEGSRERANIAVILKAGERARDLVRQILAFSRKDAPTRQPVDLAALSRES
                     LKMLRATLPATVEIEESIDDVPQILADPAQLHQIVINLVVNAAQAIGDKMGKIVFQLA
                     VAPSGALGTAVPATHLSVSDTGCGMDEATRQRIFEPFFTTKAVGEGTGLGLSVVHGII
                     AGHGGRITVASTLGEGTRFDILLPAISATEVDEIDDVDPGARPHPVRATA"
     gene            6028..>8171
                     /locus_tag="VGP50_16675"
     CDS             6028..>8171
                     /locus_tag="VGP50_16675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8029070.1"
                     /translation="MRDSPRLSPAPKADGLPDSLLRAVLDANPLPMWVEDEATLRILE
                     VNDSALCHYGYGREQFLALTAAALEAPEAASGALAASGVRHHRAADGRVIDMRLETRS
                     AEVDGHLVRLVVAIDVTGESQARAENARYRDLLDVAAEWVWEWDAAFCVSHLSPDFAA
                     ATGLLPQSFLGRRQEETAATPAAADQWTDHRAAIAAHQRFRDFVFKVADGEGRAVWLK
                     IGGTPVLGADGAFAGYRGVGSNVTAQVEAETALRENDRRFRQLFEAASDWYWENDAQG
                     RITFVSPNFEAKYGIKLVEMLGKRLGDVADAKIDPESGQKTLTAIKARQPYSDLVYSH
                     TLPDGRTVWVRTSAIPIFDTKGAFSGYLGVSKDITAEVEAERVLRESERQFKQVLEAA
                     ADYYYEQDKQYRFTHLSPGYATRFPIAESLGKRLTELPDLSVEPEMGKLVLLAQKAKQ
                     PYRDFVFARKTADGKKHWFKWSGAPLFDRNGRFAGYRGVGAEITQRVEIEAAARLAQQ
                     QRLQEAVAYVSHPIVVFDIEDRAVAFNQAFTDLHTAPDTNSPVAQGASFRELAEWQLR
                     FRFYGEGPDDTVVGLETLLARHQTEAEHSYHLRDDRWMLVVYRRLPGGGTVGLWTDVT
                     ALKRAEAERRALERQVHHAQRLEALGTLAGGVAHEINNALVPVIALTKIVARKLPDGS
                     RERRSLDVVLTGAERSRDLVKQILAFSRKEEE"
BASE COUNT         1458 a         2677 c         2734 g         1302 t
ORIGIN      
        1 aaggcctcgc gctcgccgcc gagcagaagg acgacgctgc cgtcatggcc gcgcggaccc
       61 cagaggaaat agctgttgtg gccgctgatc gccggcggca atccccaggc cgtgccgagc
      121 acgtcgacgg ccgccgcctc accatagttg cgggcgagga acaccgcttg cgtgcgctct
      181 tccgccggga gcgcctggta ggcgcggccg accagggccg cgagctcgga ccagccgaac
      241 atgtcggcgt agtattgcgg caagcggccg accgcgagcc gctccagcgg cttgggcgat
      301 tgtccgagcc gctcctcata ggcggcgaac cgcgcgaccg gcaggatcgg caaggcgaag
      361 ggcgccagga cgacgccgag gagggcgacc gccgccgcga gggcggcgcg tggcgccggc
      421 cgcgaaagcc acgcctcgag cgccgtcgcg ccgccggcga agagcacggt gtaggcgccg
      481 acgggatagt agggcttggc gtgcaccgcg atcatgacgg cgacgagcgc aatgaaggcg
      541 acggcgaaca ggcggaggtc ggcgagccgg cgccagaacg cgaaagcggc gacgccgatg
      601 agccagaccg gcgccgtccc ggggttcaga ttctcgatct cggcggcgag gaaggcgagc
      661 ggcgacaaag cgacgttctt gccttccgcg gcgcgccggc cgatctcgag gaacggccag
      721 tcatgtgccc attgccacag cacgttgggc agcacgatga ggagagcgat agcggcgccg
      781 gcatagagag ggggacgcgc cagcgagcgc cgcgcgggtg tcgcgacgat gcccagcccg
      841 agggccgcaa gccagaacac gatcatgtat tttgacagca acgcgacccc gaccaccggg
      901 cccatggcga gccaccagcg ctcgtcgcgc tcgcggatca cgcggatgag cgcgtagctg
      961 cagaacgacc aggcaagcgg ctgaagggcg tcggtcgaaa gcaaggtgcc catgccgagg
     1021 tagacgccgg cgaccaggac gcagagcgcg gcgaggccct gcccccagac gcgggcgccc
     1081 agaacgcgcg ccgtctcgcc ggtaagcgcg atcgtgccgg catgggccag cgccggcacc
     1141 aggcgcagca tgacgagcga cgccgggaac aggctgtgca tcgtccaggc gatgaggggg
     1201 atcagcggcg gctggtcgac atagccccaa tcgagacggc tgccgcagac gatgaagtaa
     1261 agctcgtcgc gaaagacgcc gtagccgccg ctggcataga gatggaggag gagcgccagc
     1321 accgccagca cccaggccgg acggtcgcgc agcaggcgga gagcagacgg catcgtcctc
     1381 gggagggctt gcgcggaagg gccgacgcta tcaccggaac gtggccgcgc tcaagccgac
     1441 gcgccgaccc tgcagaaccg gctctcctgg tccatcctga cgacccagcc gcgtcggctt
     1501 gcccgggggt gccggcgcca ttagcttccc gccgcagccc gcttggcgga acaggtagac
     1561 gcaagggact taaaatccct ttctcgcaag ggagtgccgg ttcgagtccg gcagcgggca
     1621 ccaggacttg cggccaagat gtcgactctc gtgatgggag atggtcggcg ttggccgccg
     1681 atgagcaagg tcatgtgcaa agtggctgcg gcttcggatg gagtttcaga aacgcagtcg
     1741 tggcgtccag cgcgcgacgc tcgatctccg tcggatcggg ggatttgatg acacggagca
     1801 tcagccgcat catgaggtct ctccagagaa gggcgagaaa ctctcccgcc atgtcggtcg
     1861 gatcgccggc ttcgagcagt ccggcctcct gagcctgtgc cagcacatcg ctcagcgctg
     1921 cgcgattcgg ctgactgcct tccgcgtcga gcacttgagc gacctcaggt gagcgcgtcg
     1981 cctctgtgat gcccaaacgg aagacggcca tgaccgacgg gtggcatacc tcgcgcagga
     2041 ggatggcacc aaagatccgc agaatccttg ccaacgtctc gcggtcacgt gccgccggca
     2101 ggtcaggcgg caggcgcatt ctcgtcaccc ggtcagcgat gctcgcaaca agcatggcct
     2161 ttttgttgcc aaaaagggcg tagagctctc gcttcgagac ctttgcgcgc gtggcgatct
     2221 ccagcgtgct cgtctccgca tagccgtttt ccgtaaaggc gttgaaggca gcacggagga
     2281 tgcggtcgcg gaccgggact ggcggtgccg tatcactgac acaagtcgcg agcgatttcg
     2341 ttcgcttcac aaaaaaacct gttgcgttgg taccaccaag taccgtaggg tttcgtcctc
     2401 tggtaccagt aggtaccatc tggcgattgc cgctgcaagg gctcaccgat cgcgacgatg
     2461 gctcaactct cgttcgatcg gccgccggcc atcggcgcgc cggccgacgc ccgccgcggt
     2521 catgcgcctt cggacaacga cgggagaatt gtgatgacca agatgaatcg gcggatcgcg
     2581 ctcgcgctcg caagtgtgag tgtcattgcg gctgccatag caccgacgcc agtgtttgca
     2641 gctgacgaga gtgccgcgaa cgccgcgctc ctcaagcgct acgtggccgc cttcaatgcg
     2701 catgacgccg cgggcttcaa ggacatcgtt gccgagaact atatccagca caacggccgc
     2761 gccggacaag gcctcgcggg tctccaggcg gcggccagcc agtactttca gacatttccc
     2821 gattttcaca tgcagctcga ggacagcgtg atgaacggcg acaaggtcgt cgcccgcttc
     2881 accctcacgg cgacgcacga tcacgatgtc cagctcggtc ccggcgcgcc cactttcccg
     2941 ccgacgggaa agaagctcag ctggagcggc atttcgatct ggcgggtcgc ggatggcaaa
     3001 ttcgccgagc attgggacgt cgacgatctc gccggcctgg cgcggcagat gcggggccaa
     3061 tgaccggcgc ggacaatcgg ccgcaggaag gcgatccgca ggagagatga tcgatgccga
     3121 atgtcgcgaa catgcttcag aacatgccgt ggtgggcgtt cgttctgctc gctctgcttg
     3181 tcgtgcttgg cgttcaagcg ctgcagccgc gcatgattgc cgtgtggcgc ctcctcattg
     3241 tgccggccac gttcatcggt tggggcgtga taagcctcgt gacccagtcg gttgcgtcgc
     3301 cgatcctgat ggcggattgg ctcgcaaccg cggcggcggg cgcggcgatt gcctggggcg
     3361 cgacgcggct cgagggcatg cgcatcgacc ggcccggcgg gcacgtccag attcccggca
     3421 gcgtctttcc gctcatgcgc aatctcgtca tctttgccgc gaaatatggg ctgacggtgg
     3481 cgacgacgat ggcgccgtca atgcagatga cgctttccct gtggaatatc gcggtctccg
     3541 gattgagcgc aggctatttc atcggctggc ttctccgctt tgcgctcaag taccgaacga
     3601 ctcccgagcc ccagacggga catcctgtcg gctaattcat cgaggcggtg gcacgcccaa
     3661 tcgatgccac cgacgcggcc tcaggcgatc ggccaggcgt ctctgcaagc atggccgcgc
     3721 cgccgcttag cgcttactta aggaggcctt gctaacctgc ttcgccgtgc agtcaagcat
     3781 gggctccggg tgatggcttt tctcgaccgc tgtcggatca aggtgaagct cgccttgatc
     3841 gtcggcgtct gcgcgctggc cttggtcgcc gcgttggccg ccgccgcgga gatgcagcgt
     3901 cagcgcatga tctccgatcg gatcgacaag gcccgtgcca tcgtcgagat cgcctatggc
     3961 ctcgccgatt cgctggagcg cgaggtcgtc gcccggcacc tcacgcatga cgaggcgctc
     4021 gcccgtttca ccgacgatct gcgcgccatg tggtacgaca atcatcagaa ctacatcgtc
     4081 ctgggctcgc tcgacaacgt ctggatgatc catcccgcgg tcccccgggt cgagggcacg
     4141 atcggctcga ccgacctcga gaatccgagc ctctatctca tcacgctgtt caccgacgcc
     4201 gtccgctcgg tcgatgaagc gatcgtgcgc taccgctatc cccgcccggg acagaaagag
     4261 cggttgctga agatcacctt taccaagaag ttcacgccgt ggaacgcgtt catcgccagc
     4321 ggtgcctatg tcgacgacat cgaggccgaa tatcgatcgg tgctcggccg gctcgccttg
     4381 atcgggctcc tgatcatgac ggcggccgcg gcgaccgcct atgcgatcag ccgcaacatc
     4441 agccactcgc tcactgcctt gaaggggcga atggagcaat tggccgcggg cgacctgacg
     4501 gtcgatatcc gcgaggccgg ccgccaggac gagatcggcg agatggcgcg gacggttcag
     4561 gtattcaagg acaatgcgct cgagaaagag cgcatgcacg gcgaattgca gcacaaggag
     4621 gaattgcgaa ggacggccga gagcctccgc cgcagccaag agcgcttggc ccaggcccag
     4681 cgcctcgccg gcatgggcag cgacttcagg gatatcgcca acgatgtcgc cgactggtcg
     4741 gacgagacct atcggatctt cggcgtcgcc cgcgagacct tcacgccgac cacggagggc
     4801 cttctctccc tgatccatcc cgacgaccgg gggctcgtcc tggcagcgcg cggggaggtc
     4861 gtgcgaggga tatgcccggc tccgttcgat caccggatcg tccggcgcga tggcagcttg
     4921 cggatcgtcc gccgcgagac ggagctgctg cgcgacgaga ccggcacggt cgtgtcgctc
     4981 gccggcacga cgcacgacgt cacagaacag cgcgccgccc agaagcgtca gagcgagctc
     5041 gagcggcagg tcctgcattc gcaaaagctc gatgcgctcg gcacgctcgc cggcggcatt
     5101 gcgcatgacc tcaacaacac gctcgtcccg gtgttgggtc ttgccaagct cacgatgggg
     5161 cgcatggccg aaggcagccg cgagcgcgcc aatatcgccg tgatcctgaa agcgggcgag
     5221 cgtgcgcgcg acctcgtccg tcagattctc gccttcagcc gcaaggatgc gccgacgcgc
     5281 cagccggtcg atctcgcggc cttgtcgcgc gagtcgctga agatgcttcg cgcgaccctg
     5341 cctgcgaccg tcgagatcga ggagtcgatc gacgacgtgc cgcaaatcct cgccgacccc
     5401 gcgcagctgc accagatcgt catcaacctc gtcgtcaatg ccgcacaggc gatcggcgac
     5461 aagatgggaa agatcgtctt tcagctcgcc gtggcgcctt cgggtgcgct gggcacggcc
     5521 gttccggcca cacatctctc agtcagcgac accggctgcg gcatggacga agcgacgcgg
     5581 cagcgcatct tcgagccgtt cttcaccacc aaggcagtgg gcgaaggcac cggcctcggc
     5641 ctctccgtcg tccatggcat catcgccggg catggcggcc gcatcaccgt ggcgagcacg
     5701 ctcggcgagg gcacccgctt cgacattctt ctgcccgcga tctccgccac ggaggtcgac
     5761 gagatcgacg acgtcgatcc gggtgcacgc ccccatccgg ttcgagcgac cgcctgaggc
     5821 ggttttttct ttgtccattg aggattcatc ctcatcttca tcttaagccg gcgccgaagg
     5881 cgtctgagtg cctccgcgag gacgccttgc cagcacggtt gcgccatacc cgagtcggtc
     5941 gccggctccc tgatcgctaa gctttgcgca atcactgtat ggaagtatgg gagcccggtc
     6001 gcagaggatg acgcacggat gcccacaatg cgggacagcc ccaggctctc gcccgcaccc
     6061 aaggcggatg gcctgcccga cagcctctta cgcgccgtgc tcgacgcgaa ccccctgccg
     6121 atgtgggtgg aggacgaggc gacgctgcgc atcctcgagg tcaacgacag cgcgctctgc
     6181 cattacggct acggccggga gcaattcctc gctttgaccg ccgccgcgct ggaggcgccg
     6241 gaagcggcgt ccggcgcctt ggcggcgagc ggcgtccggc atcaccgcgc ggccgacggc
     6301 cgggtgatcg acatgcggct cgagacgcgc agcgccgagg tcgacggcca cctggtccgg
     6361 ctggtcgtcg ccatcgacgt caccggagag agccaggcac gcgccgaaaa tgcccgctac
     6421 cgagacctac tcgacgtcgc agcggagtgg gtgtgggaat gggacgccgc gttctgcgtc
     6481 agccatctct cgcccgactt cgcggcggcc accggtctct tgccgcaatc cttcctgggc
     6541 cggcggcagg aggagacggc ggcgacccca gcggcggccg accaatggac cgatcaccgc
     6601 gcggcgatcg cggcgcacca gcgcttccgc gatttcgtct tcaaggtcgc ggacggagag
     6661 ggacgcgcgg tctggctcaa gatcggcggg acgccggtgc tcggcgccga cggtgccttc
     6721 gccggctatc gcggtgtcgg cagcaacgtc acagcgcagg tcgaggccga gacggcgctg
     6781 cgcgagaacg atcggcgttt ccggcagctg tttgaagcgg cctccgactg gtactgggaa
     6841 aacgatgcgc aagggcgcat tacctttgtc tcaccgaatt tcgaagccaa gtacggcata
     6901 aagctcgtcg agatgctcgg caagcgtctc ggtgatgttg ccgatgcgaa gatcgatccc
     6961 gaatcagggc agaaaacgct gactgcgatc aaggcgcgcc aaccctatag cgacctcgtc
     7021 tattcccaca ctttacccga cggcaggacc gtctgggtca gaacgagcgc cattccaatc
     7081 ttcgatacca agggcgcgtt cagcggctat ttgggcgtct ccaaggacat caccgccgaa
     7141 gtcgaggctg agcgcgtgct ccgcgagagc gagcggcagt tcaagcaggt gctggaggcg
     7201 gccgccgact actactacga gcaggataag caataccgct ttacgcatct ctcgccgggt
     7261 tacgcgacac ggttccccat tgccgaatcg ctgggcaagc gactcaccga gcttcccgac
     7321 ctgtcggtcg agcccgagat gggaaagctg gtgctgctgg cgcaaaaggc gaaacagcct
     7381 taccgcgact ttgtcttcgc gcgcaagaca gccgatggca agaagcattg gttcaagtgg
     7441 agcggcgcgc ccctgttcga ccggaacggc cggtttgccg gatatcgcgg cgtcggcgcg
     7501 gagatcacgc agcgggtcga aatcgaggcg gcggcgcggc tggcgcagca gcagcgattg
     7561 caggaggcgg tcgcgtatgt cagccatccc attgtcgtct tcgacatcga ggatcgggcc
     7621 gtcgccttca accaggcctt caccgacctg catacggcgc ccgataccaa ctcgccggtg
     7681 gcccagggcg cctccttccg ggaattggcc gaatggcagt tgcgcttccg cttctacggc
     7741 gagggcccgg atgataccgt ggtcggtctc gagacgctgc tcgcccgcca tcagaccgag
     7801 gcggagcaca gctatcattt gcgcgacgat cgctggatgc ttgtcgtcta tcgccgcctg
     7861 ccgggcggag gcacggtcgg gctgtggacg gatgtgacgg cgctgaagcg ggccgaggcg
     7921 gagcggcgcg cactcgagcg ccaggtgcat cacgcgcagc ggctggaggc gctgggcacg
     7981 ctcgcgggcg gcgtcgcgca cgagatcaac aacgccctcg ttccggtgat cgcgctcacc
     8041 aagatcgtgg cgcgcaagtt gccggacggc agccgcgagc ggcgcagcct cgacgtcgtc
     8101 ctcaccggcg ccgagcgctc acgcgacctg gtgaagcaga tcctcgcctt ctcgcggaag
     8161 gaggaggagg a
//
LOCUS       DASYNS010000541         3931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2632712, whole genome shotgun sequence.
ACCESSION   DASYNS010000541 DASYNS010000000
VERSION     DASYNS010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2632712"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..929
                     /locus_tag="VGP50_16680"
     CDS             132..929
                     /locus_tag="VGP50_16680"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEV8029071.1"
                     /translation="MPALHEMQAAFRRALIEGDAAALVALVEADDIGASERVAIYRNN
                     VACALTDVLRDTFPAVCRLVDGQFFAYAADAFIQRHPPERACLAEYGARFPDFLADFP
                     PCRELVYLPDVARLEWLMNVAAHAEDTDALAASALAGIATAEAPRLVFRLAPALGLLA
                     SPWPVDRIWRANRPAAGQEETIDLGEGGVRLEIGRHHGAVVLRQLDAATFAFRDAIAR
                     GATLAAATRAVLSVDGRFDLEAALAALFAEGAVVASSVAPETAHASL"
     gene            1005..>3931
                     /locus_tag="VGP50_16685"
     CDS             1005..>3931
                     /locus_tag="VGP50_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029072.1"
                     /translation="MRRIIAAAIAAMALGAGSAAAQGLDVLTHHNDIFRSGVYSAETS
                     LSPSSVSPPRFGKLFSRNIIGQVWGQPLYVHGVSIAGALRNVVYVATSENWVYGFDAD
                     NLSPDEGTAPLAKIQLGPPAAIGGDICDTGFHTVSPTIGVISTPVIDPASLTLYVVAR
                     IKTQYQVFALDLATLSVRKNVTVSGSASNIAFDPSCQMTRPALLLSAGHLVVAFGSGP
                     ATDNVAYHGWVMSYSVPNLTQTGVFNTTPYSWNYSQNGLTGSGSIWQSGNGPAADEQG
                     NVYVMVGNGMPPPPGSALPDVTDSIVKLANSDGSLRLADWYTPPSRRALQLCDLDLGS
                     SGPAILPAFGKVLGAGKSAVLYVLDAAQMGGTDTPLADPGAWTGAPDCTKGQCFRVGT
                     NRNSPPSGDAQQPCGNGSNWNNVLDSYPHVHGAPVAWTRAPNQVNLYVWPEEDFLGVY
                     RFNGQQFSAASIGSSAPITAAMESMPGGILSLSWDGKRPASAIVWASRPYPFGNGART
                     QCFDGGDNYPKDLAPNNAPCNAIDKIVDGYLEAFIADPDADGRLVEVWNSKMLLSDNV
                     DWFAKNSPPSIAEGKVFLAAFPPNNGPNSYSQPGATGHLVVFGAKTPVAAKSRIDRYW
                     GPSDQQHVNFIDTKGHVRELFIDTGRGWVDNDLMLLTRSATAAAPTGGIAGDFGDDQQ
                     HVNFLDTRGHVHELYYPQNGPWQDNDLMGVARSATIAATGSPVDRYRGPGDQQHVNFI
                     AGNGHVHELFIDSGRNWIDNDLMQQSHSTTVAAASGGMAGDVGGDSQQHVNFLDGKGH
                     VHELYYPQGGPWQENDLMASAHTSTTAAPGSAVARYWSSSTQQHVDFIDGSGHVHELL
                     IDSGHGWIDNNLMTLAKTATVASASGGLDGYVTGDGAQHVNFVDADGRVHELYFPQGS
                     SWRDNDLTVLANSSANAAPSSPLAGDRGNDNDQQHVNFFDVNGQVDELWIDTGRSWAE
                     NGLR"
BASE COUNT          660 a         1406 c         1230 g          635 t
ORIGIN      
        1 tcgccgcgcg cttgggcgct tcggcggcgt gccaagcctc gtcgagtggg acaaggcgct
       61 gccgccgcta tgcgcattgc tcgacgaagc gcgcgcggcg gagcgcatcg cgcgcgcaga
      121 ggaagacggc gatgcctgcg ctgcatgaaa tgcaggcggc cttccgccgc gctctcatcg
      181 agggcgacgc ggcggcgctc gtcgcgcttg tcgaggcaga cgacatcggc gcgagtgaac
      241 gggtcgcgat ctaccgcaac aatgtcgcct gcgctctgac ggacgtgctg cgcgacactt
      301 ttcctgcagt atgccgcctc gtggacgggc agttcttcgc ctatgcagcc gacgccttca
      361 tccagcgcca tccgccggag cgtgcctgcc tcgccgaata cggggcgcgc tttcccgact
      421 ttctcgccga ttttccgccc tgccgggagc tcgtctatct cccggatgtc gcgcggctcg
      481 aatggctcat gaatgtcgcc gctcatgccg aggacacgga cgcccttgcc gcatcggcgc
      541 tggccggcat cgctaccgcc gaggcgccgc ggcttgtctt ccgcctcgcg cctgccctcg
      601 gccttctcgc atcgccctgg cccgtcgatc gcatctggcg cgcgaaccgg ccggcggccg
      661 ggcaggagga gacgatcgat ctcggcgagg gcggtgtccg cctcgagatc ggccgccacc
      721 acggcgcggt ggtccttcgc cagctcgacg cggcgacctt cgcgttccgc gacgccatcg
      781 cgcgcggtgc gacgctggcc gccgcgaccc gcgcggtgct gtcggtcgat ggacgcttcg
      841 atctcgaggc ggcgcttgcc gcgctcttcg cggaaggcgc cgtcgtcgcc tcgagcgttg
      901 cgccagagac tgctcacgca tcgctgtgac gcaagtcaca tcgcaagctc tgccgatcag
      961 gtgtttaatg gcccctgacc gaccagcgtc gggaggggaa aagcatgcgc aggatcatcg
     1021 cggccgccat cgcggcgatg gcgttgggcg ccggctcggc ggcggctcag ggcctcgatg
     1081 tcctcactca tcacaacgac atctttcgca gcggcgtcta tagcgccgag accagcctca
     1141 gcccgagctc ggtgagcccg ccgcgcttcg gcaagctctt ctcgcgcaac atcatcggcc
     1201 aggtctgggg acagccgctc tatgtccacg gcgtctcgat cgccggtgcg ctccgcaatg
     1261 tggtttatgt cgcgacatcg gagaactggg tctacggctt cgacgccgac aacctctcgc
     1321 ccgacgaagg cacggcaccg ctcgccaaga tccagctcgg ccctccggcg gccatcggcg
     1381 gagatatctg cgacaccggc ttccacaccg tgtcgccgac catcggtgtc atcagcacgc
     1441 ccgtgatcga cccggccagc ctcacgctct acgtggtcgc gcgcatcaag acgcagtacc
     1501 aggtcttcgc gctcgatctc gcgacgctca gcgtgcgcaa gaacgtcacc gtctccggca
     1561 gcgcgagcaa cattgccttc gacccgtcct gtcagatgac gcggccggcg ctgctgctgt
     1621 cggccggcca tctcgtcgtc gccttcgggt ccggccccgc caccgacaat gtcgcctatc
     1681 acggctgggt catgtcctat tcggtgccaa atctgacaca gacgggtgtc ttcaacacca
     1741 cgccctacag ctggaactac agccagaacg ggctcaccgg cagcggcagc atctggcaga
     1801 gcggcaacgg gccggcggcg gacgagcaag ggaatgtcta cgtcatggtc gggaacggca
     1861 tgccgccgcc gccgggctcg gcgcttcccg acgtcaccga ctccatcgtc aagctcgcca
     1921 acagcgacgg ctcgctgcgc ctcgccgact ggtacacgcc gccctcgcgc cgcgccttgc
     1981 agctctgcga tctcgatctc ggctcgtcgg ggccggcgat tctgcccgcg ttcggcaaag
     2041 tgctcggcgc cggcaaatcg gccgttctct acgtcctcga cgccgcgcag atgggcggca
     2101 cggacacgcc gcttgccgat cccggcgcct ggaccggagc gcccgactgc accaaggggc
     2161 aatgcttccg cgtcggcacc aaccgcaaca gcccgccgtc cggcgacgcg caacagccgt
     2221 gcggcaacgg ttccaactgg aacaacgtgc tcgacagcta tccgcatgtg catggcgcgc
     2281 cggtggcctg gacgcgcgcg ccgaatcagg tgaatctcta tgtctggccc gaggaggatt
     2341 tcctcggcgt ctaccgcttc aacggccagc aattctcggc cgccagcatc ggctcctcgg
     2401 cgccgatcac cgcagcgatg gagagcatgc cgggcggcat cctgtcgctc tcctgggatg
     2461 ggaagcgccc ggccagcgcc atcgtctggg cgtcgcggcc ctaccctttc ggcaatggcg
     2521 cgcgcacgca atgcttcgac ggcggcgaca actacccgaa ggacctcgcg cccaacaacg
     2581 cgccctgcaa tgccatcgac aagatcgtcg acggctatct cgaagcgttc atcgccgatc
     2641 ccgacgcaga tgggcggctc gtcgaagtct ggaacagcaa gatgctgctc agcgacaatg
     2701 tcgactggtt cgccaagaac agcccgccca gcattgccga gggtaaggtg ttcctcgcgg
     2761 ccttcccgcc gaacaacggc cccaacagct acagccagcc cggcgccacc ggccatctcg
     2821 tcgtcttcgg cgcgaagacg ccggtcgccg cgaagagccg aatcgaccgc tactgggggc
     2881 ccagcgacca gcagcatgtc aacttcatcg acaccaaggg ccatgtccgt gagctcttca
     2941 tcgacaccgg ccgcggctgg gtcgacaacg acctgatgct gctcacgcgt tcggcgacag
     3001 cggcagcgcc gacgggcggc attgccggcg acttcggcga cgaccagcag catgtgaact
     3061 tcctcgacac cagaggtcat gtgcacgagc tctactaccc gcagaacggg ccgtggcagg
     3121 acaacgatct catgggcgtc gcccgctccg cgacgatcgc ggcgacaggc agcccggtcg
     3181 accgctaccg gggtcccggc gatcagcagc acgtgaactt catcgccggc aacggccatg
     3241 tccatgagct cttcatcgac agcggccgca actggatcga caacgacctg atgcagcagt
     3301 cgcattcgac gacggtcgcc gccgcctcgg gcggcatggc cggcgatgtc ggcggcgaca
     3361 gccagcagca tgtgaatttc ctggacggca aaggccatgt tcacgagctc tactatcccc
     3421 aaggcggtcc atggcaagag aacgatctca tggcgtcggc gcacacatcc acgacagcgg
     3481 cgcccggcag cgcggtcgcc cgctactgga gctccagcac tcagcagcat gtcgatttca
     3541 tcgacggcag cggtcatgtg catgagctcc tcatcgacag cggccatggc tggatcgaca
     3601 acaacctgat gacccttgcc aagacggcga ccgtcgcgtc cgcgagcggc ggcctcgacg
     3661 gctatgtcac gggggatggt gcccagcatg tcaacttcgt cgatgccgac ggccgcgtcc
     3721 acgagctcta ctttcctcag ggcagttcgt ggcgggacaa cgatctcacc gtgttggcca
     3781 attcctcggc gaacgcagcg ccgtcctcgc cactcgccgg cgatcgcggc aacgacaatg
     3841 atcagcagca cgtcaacttc ttcgacgtca acggccaggt cgacgagctc tggatcgata
     3901 ccggacgaag ctgggccgag aacggcttgc g
//
LOCUS       DASYNS010000542         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1226699, whole genome shotgun sequence.
ACCESSION   DASYNS010000542 DASYNS010000000
VERSION     DASYNS010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226699"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VGP50_16690"
     CDS             <1..356
                     /locus_tag="VGP50_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029073.1"
                     /translation="RLVELAVALGLEPRVLLLDEPAAGVPSSESGIIIAAIERLPPEI
                     ALLIIEHDMELVFRLAERITVLVQGRVLVEGTPQEIAADPRVREVYLGEHSAMRSIVA
                     GGRERALLRPQEPAP"
     gene            353..1069
                     /locus_tag="VGP50_16695"
     CDS             353..1069
                     /locus_tag="VGP50_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV8029074.1"
                     /translation="MTGTGIVLEDLRAGYGETIVLDGVSLSLPPRGTLAVLGRNGVGK
                     TTLLATIMGHTTRHGGSIGFDGRDMTRLAPHRRARAGIGWVPQEREIFRSLTVEENLA
                     VAARPGRWSAARIWDLFPSLGERRRHFGNELSGGEQQMLAIGRALMGNPSLLLMDEPL
                     EGLAPVIVEVVLRSIERLKREDDLAILLVEQHARLALAATERAIVLDRGKIVHAGESR
                     ALLAAPEQLGALMGIERRTH"
     gene            1193..1690
                     /gene="pal"
                     /locus_tag="VGP50_16700"
     CDS             1193..1690
                     /gene="pal"
                     /locus_tag="VGP50_16700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEV8029075.1"
                     /translation="MNGMMKGLAVAAALTALQGCSWLFGDDKGKVVGNNEERPAVEQQ
                     VDRQQANHESDLISFDTIYFDYDKTTLRAEALPVLTHLAKWMSEHPDVRIRIAGHADE
                     RGTREYNLALGDRRATAVRNYLASIGVPGQRLDTVSYGKERPAVVGSSEESWAKNRRA
                     VAELE"
BASE COUNT          315 a          673 c          696 g          304 t
ORIGIN      
        1 agcgcctggt cgagctcgcc gtggcgctcg ggctcgagcc cagggtgctg ctgctcgacg
       61 agccggccgc cggcgttccg tcgtcggaaa gcggcatcat catcgcggcg atcgagcggc
      121 tgccgccgga gatcgccctc ctcatcatcg agcacgacat ggagctcgtc ttccgcctgg
      181 cggagcgcat caccgtgctg gtgcaaggcc gcgtgctcgt cgaaggaacg ccacaggaga
      241 tcgccgccga tccccgcgtg cgcgaggtct atctcggcga gcacagcgcg atgcgcagca
      301 tcgtcgcggg cgggcgcgag cgggcgctct tgcgaccgca agagcccgca ccatgaccgg
      361 caccggcatc gtgctcgagg atttgcgtgc cggctacggc gagacgatcg tgctcgatgg
      421 cgtgtcgctg tccctgccgc cgcgcggcac cctcgccgtg ctgggccgca acggcgtcgg
      481 caagacgacg ctgctggcca ccatcatggg gcacacgacg cgccatggcg gcagcatcgg
      541 tttcgacggc cgcgacatga cccggcttgc gccccatcgc cgcgcccgtg ccggcatcgg
      601 ctgggtgccg caggagcgcg agatcttccg ctcgctgacc gtcgaggaga atctggcggt
      661 ggcggcgcgg cccggccgct ggtcggcggc acggatctgg gatctctttc cctcgctcgg
      721 tgagcgccgg cgccacttcg gcaacgaatt gtcaggcggc gagcagcaga tgctggccat
      781 cggccgcgca ctcatgggca atccgtcgct gctgctgatg gacgagccgc tcgaagggct
      841 ggcgccggtc atcgtcgagg tcgtgctccg cagcatcgag cggctgaagc gcgaggacga
      901 tctcgctatc ctgttggtcg agcagcatgc gcggctggcg ctcgccgcga ccgagcgcgc
      961 catcgtcctc gaccgcggca agatcgtcca tgccggagag agccgcgcgc tccttgcggc
     1021 accggagcag ctcggcgcgc tcatggggat cgagcgccgc acgcactgag tctcgccaag
     1081 aaattgccca gaagtctccc cgcgatcgcc acaggcgcgt cataccgggc cacactcctc
     1141 tgcccatagt gacgccggtc ttcggaacct gggttttcac ggaggaggta tcatgaacgg
     1201 tatgatgaaa ggactggcag tggccgccgc gctgacggcg ctgcagggct gctcttggct
     1261 ctttggcgac gacaaaggca aggttgtcgg caacaacgag gaacgtccgg cggtcgagca
     1321 gcaagtcgac cggcagcagg ccaaccacga gagcgatctc atcagcttcg acaccatcta
     1381 cttcgactac gacaagacga cgctgcgcgc cgaggcactg ccggtgctga cgcatctggc
     1441 gaaatggatg agcgagcatc cggatgtgcg tatccgtatc gcgggccatg ccgacgagcg
     1501 cggcacgcgg gaatacaatc tggcgctggg cgatcgccgc gccacggcgg tgcgcaacta
     1561 cctcgcctcg atcggcgtgc cggggcagcg gctcgacacc gttagctatg gcaaggagcg
     1621 gccggcggtc gtcggctcca gcgaagagtc ctgggcgaag aaccgccgcg cggtggcgga
     1681 gctggagtag gtgcaagacg gcgctctcgc ggttgctacg caaccactgc cgcgtcacgg
     1741 acgaacgcgc agagcgcgtt cgccgagggc ttgccggggg cgaggggcgg gatatccttc
     1801 ccgctccttt cgctatgccg ggaaccgcca tgaagccgcg ccagcgcgcc ccttattccg
     1861 ccatcgtcga ccgcccgccg ctgcgcctgc ccgagggcgt gcggctcgtc atctggccga
     1921 tcgtcaacct cgaggaatgg gagatcgagc gtgccatggc gcggctggtg ctgccgccgc
     1981 cgatgggc
//
LOCUS       DASYNS010000543         6393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2948345, whole genome shotgun sequence.
ACCESSION   DASYNS010000543 DASYNS010000000
VERSION     DASYNS010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2948345"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="VGP50_16705"
     CDS             <1..97
                     /locus_tag="VGP50_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8029076.1"
                     /translation="EEIAALALYLASDESRFTTGAIHIIDGGWTI"
     gene            118..963
                     /locus_tag="VGP50_16710"
     CDS             118..963
                     /locus_tag="VGP50_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV8029077.1"
                     /translation="MKLLRWGPRGQEKPGMLDRDGVLRDLSGVVADINPETLAPAALD
                     KLRKLDAAKLQVVPGKPRLGPCVAQVPKLVCVGLNYVDHAKEAGMQVPGEPVLFMKAT
                     TAISGPNDEVMLPKGAQKGDWEVELGIVIGAKTQYVGVQEALRHVAGYCVVNDVSERS
                     FQLERGGNWSKGKSADTFCPLGPWLVTADEVPDPQALDLFCEVSGQRMQNGSTRNMIF
                     PCAHIVSYISHFMTLLPGDVIPTGTPAGVGIGMKPQRFLKPGDVMRLGVSGLGEQRQT
                     VVAYG"
     gene            1108..1626
                     /locus_tag="VGP50_16715"
     CDS             1108..1626
                     /locus_tag="VGP50_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEV8029078.1"
                     /translation="MPERRPGFFRTEDAPTGRRVEAKVKWFSATKGFGFVALSDGSPD
                     AFLPMATLRRAGFEDAREGASISCEVGTGAKGPLVVTVLGIDNGTAAPAGAGRPATPR
                     SAQTVEGVVKWFEPDKGYGFIAPDGGGKDVFVHVTALRRSGVDAIGPGQRVRVDVVDG
                     RKGYEADRVTLL"
     gene            1792..2418
                     /locus_tag="VGP50_16720"
     CDS             1792..2418
                     /locus_tag="VGP50_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV8029079.1"
                     /translation="MELEDTLKTYRLFSGSYDLLFGPVFHPGRKDAVRIANDRPAQRI
                     LEVGVGTGLSLPHFRADARVTGIDVSTEMLAKARRRVAQKNLKQVEALLEMDAENMTF
                     PDSSFDAVLALYVASVVPDPARFAAEIRRVCIPGGTIVVVNHFMSENAPMRFIEKRLA
                     PLAGKIGFHADFPLDGFLKVSKLQVREIRPSNLFGYWKLLRCVNVKPA"
     gene            complement(2399..2770)
                     /locus_tag="VGP50_16725"
     CDS             complement(2399..2770)
                     /locus_tag="VGP50_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029080.1"
                     /translation="MRVSPLILLFLVCAATRAEAGTVVLVSSSPYWQVGVRDNELSRA
                     CSLDAFGPKRLGALVARFTGPEGPALLDVAKGSGNNLRDPKHLAKRNEDYFFRNAATT
                     SCEVLVGGRGGSGKPVTPASR"
     gene            2895..3431
                     /locus_tag="VGP50_16730"
     CDS             2895..3431
                     /locus_tag="VGP50_16730"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEV8029081.1"
                     /translation="MRNTGHMGKSGLRAGAVVVLAALLIGPGAGHTAPSAVKLADRIE
                     VFKAEHRLVLLRDGKPVKSYRVSIGTNPVGPKREQGDHRTPEGSYVIDFHKRDSAFHL
                     ALHISYPSEEDRRQAALRGVSPGGSVMIHGLPNDWPGFAVIHRYHDWTDGCIAVTDRE
                     IEEIAAAVPDGTPIVIHP"
     gene            3584..4063
                     /locus_tag="VGP50_16735"
     CDS             3584..4063
                     /locus_tag="VGP50_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029082.1"
                     /translation="MDPDARQPSDPAAAPSTQLVLELYKAYLTDLGNIGSRYTTYNGF
                     YVSIVSGLLAVLALTKRGEALEKFDTLLYLVIPIFAGLLCLVWRETMTFHSALFRTKF
                     DVLREMEEYLPFKAYTREEKLRRPGRRLIKNDELIPVILALPFAVVFIFGLWKLLQR"
     gene            complement(4211..6001)
                     /locus_tag="VGP50_16740"
     CDS             complement(4211..6001)
                     /locus_tag="VGP50_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV8029083.1"
                     /translation="MTDYAAPLADMRFALNEIAGIAEIAALPGCEQASADLVDTILEE
                     AGKLAAGVIAPLNRIGDRQPSRLENGVVRTPPGFKEAWAEHVAGGWNALPFPTEHGGQ
                     GLPFALATAVAEMWNAANMSFALCPLLTIGAVEALAAHGTPEQQRLYLPKLVSGEWTG
                     TMNLTEPQAGSDVGALRTRAERAGDHYRITGQKIFITYGDHDMAENIVHLVLARLPGA
                     PAGTRGISLFLVPKFLVNSDGSLGARNDVRCVSLEHKLGIHASPTCVLAYGDAGGATG
                     FLVGEENRGMECMFTMMNNARLNVGLQGVAIAERAYQQARDFARQRVQGKPVGATGSG
                     TPPIIHHADVRRMLLAMRADTEAMRALSYYTAGAVDRARHHPDTETRALQQRRVDLLT
                     PVVKAWCTDLGVAIASTGVQVHGGMGFIEETGATQHLRDARILPIYEGTNGIQANDLV
                     GRKVLRDSGLAVRELIAEMRATDGALANVPGAALAAIRAPLKAAIDALDVATRWLVEH
                     GTAEPARVLAGAVPYLKLMGNVTGGWLMARGALASERALAQGNGQAPFHRAKLISAQF
                     YTEHVLATAPSLLPAVAGGSTVLEFDLDQF"
BASE COUNT         1046 a         2089 c         2160 g         1098 t
ORIGIN      
        1 ggaagagatc gcggcgctcg ctctttacct cgcgagcgat gaatcgcgct tcaccaccgg
       61 cgccatccat atcatcgatg gcggctggac catctgacaa aggggaggaa cgagacgatg
      121 aagctgcttc gctggggccc gcgcggccag gagaaaccgg gcatgctcga tcgcgacggc
      181 gtgctgcgcg atctttcggg tgtcgtcgcc gacatcaatc ccgagacgct ggcgcccgcc
      241 gcgctcgaca agctcaggaa gctcgatgcg gcaaagctgc aggtggtccc cggcaagccg
      301 cgcctcggtc cttgcgtcgc gcaagtgccg aagctcgtct gcgtcgggct caactatgtc
      361 gaccacgcca aggaagccgg catgcaggtg ccgggcgagc cggtgctgtt catgaaagcg
      421 acgacggcga tcagcgggcc caatgacgag gtcatgctgc ccaaaggcgc gcagaagggc
      481 gattgggagg tcgagctcgg catcgtcatc ggagccaaga cgcaatatgt cggcgtccaa
      541 gaggcgcttc gccatgtcgc cggctattgc gtcgtcaacg acgtctccga gcgcagcttc
      601 cagctcgagc gcggcggcaa ctggtcgaag ggcaagagcg ccgacacctt ctgcccgctg
      661 gggccctggc tcgtcactgc cgacgaagtg cccgatcccc aggcgctcga tctcttctgc
      721 gaggtgagcg ggcagcgcat gcagaacggc tcgacccgca acatgatttt cccgtgcgcg
      781 cacatcgtca gctacatcag ccatttcatg acgctgctgc ccggcgacgt catcccgacg
      841 gggacgccgg ccggcgtggg catcggcatg aagccgcagc gctttttgaa gccgggcgac
      901 gtcatgcggc tcggcgtcag cgggctcggc gagcagcgcc agaccgtggt tgcttatggg
      961 tagagctgac cggtcgcgtc gaacgcctcg ggctgccccg aaccagagcg gcgtgagtgg
     1021 cggcgcattc tgaacacgtt gcgctagcgt tggcctccgg tttcacgtta ttgtcagaaa
     1081 attgcttctt ttggttaacg aggccccatg ccagagcgcc gccccggatt tttccggacc
     1141 gaggatgccc cgaccggccg ccgggtcgag gccaaggtca aatggttcag cgcgaccaaa
     1201 ggcttcggct tcgtggcgct ctcggatggc agtcccgatg cgttcctgcc catggccaca
     1261 ttgcgccgtg cgggattcga ggatgcgcgc gagggcgctt cgatcagttg cgaagtcggc
     1321 accggcgcca aagggccgct ggtcgtgacc gtgctcggaa tcgacaacgg cacggcggcg
     1381 ccggccggcg ccggccgtcc cgccaccccg cgctccgcgc aaaccgtcga aggcgtcgtc
     1441 aaatggttcg agcccgacaa gggctatggc ttcatcgcgc ccgatggcgg cggcaaggac
     1501 gtgttcgtcc atgtcaccgc cttgcgccgg agcggcgtcg atgcgatcgg ccccggacag
     1561 cgtgtgcggg tcgatgtcgt ggacggccgc aagggatacg aggcggatcg cgtgacgctg
     1621 ctttgagcgg ccgccgcccg gcggccggac atgccggctg gcaatttgtc cgatcggtgt
     1681 taccttaatt agcgtacagg cggcccagcg ccgcctttcg tgacctgcaa ggacgctgct
     1741 cccgacgcgt accctgccgg caatccagat aaatcccgtg gggggaaggc gatggaactc
     1801 gaagacacgc tcaagacgta ccggctgttt tccggatctt acgatctgct cttcggcccg
     1861 gtgtttcatc cgggacgcaa ggacgccgtg cgcatcgcca atgaccggcc ggcgcagcgg
     1921 atcctcgagg tcggcgtcgg caccggcttg tcgctcccgc atttccgcgc cgacgcgcgg
     1981 gtgacgggga tcgacgtctc gaccgagatg ctggctaagg cgcgccgccg cgtcgcgcag
     2041 aagaatctca agcaggtgga agcgctgctc gagatggacg ccgagaacat gaccttcccc
     2101 gacagctcct tcgatgccgt gctggcgctc tatgtcgcct cggtggtgcc ggaccccgcg
     2161 cgctttgccg ccgagatccg gcgcgtctgc atccccggcg gcaccatcgt cgtcgtcaac
     2221 catttcatga gcgagaacgc gcccatgcgc ttcatcgaga agcgcctggc gccgctggcc
     2281 ggcaagatcg gcttccatgc cgacttcccg ctcgacggtt tcctgaaagt gagcaagctg
     2341 caggtccgcg agatacggcc cagcaatctc ttcggctatt ggaagctctt gcgctgcgtc
     2401 aacgtgaagc cggcgtgacc ggcttgccgc tgccgccgcg gccgcccacc agcacctcgc
     2461 aactcgtcgt cgcggcattg cggaagaagt aatcctcgtt gcgcttggcg aggtgcttgg
     2521 ggtcgcggag gttgttgccg ctgcccttgg cgacatcgag caatgccggg ccttcggggc
     2581 cggtgaagcg ggcgacgagc gcgcccagcc gcttcgggcc gaaggcgtcg agcgagcagg
     2641 cgcgactcag ctcattgtcg cgcacaccga cctgccaata gggcgacgat gagaccagca
     2701 cgaccgtgcc ggcctccgcg cgcgtggcgg cgcagaccag gaacagcagg atgagtggcg
     2761 agacgcgcat ggcaggatcg gacctgcgcc atcatcgcgc ccggatggta aacaacggct
     2821 caacggccta ttcgaattgt taagggaacc cggctaacct gggggcgatt tgtcttgcgg
     2881 caggttttcg cgccatgcgc aacacagggc acatgggcaa gtcgggattg agagccggcg
     2941 cagtcgtggt gctggcggca ttgctgatcg gcccgggcgc cggtcacacc gcgccatcgg
     3001 ccgtgaagct ggccgaccgg atcgaagtct tcaaggccga gcaccggctg gtgctcttgc
     3061 gggacggcaa gccggtcaag agctatcgcg tgtcgatcgg cacgaacccc gtcgggccca
     3121 agcgcgagca aggcgatcac cgcacgcccg agggaagcta cgtcatcgat ttccacaagc
     3181 gcgacagcgc cttccatctg gcgctgcaca tctcctatcc gagcgaggag gaccgccgcc
     3241 aggcggcgct ccgcggcgtc tcgccgggtg gttcggtgat gatccacggc ctccccaacg
     3301 actggcccgg cttcgccgtg atccaccgct accacgactg gaccgacggc tgcatcgccg
     3361 tcacggatcg cgagatcgag gaaatcgccg ccgccgttcc cgacggcacg ccgatcgtca
     3421 tccatccctg aggatcgatc agcatcgacg cgacggttgg gccacgctcc ggttttcagc
     3481 ggacagcccg cgaaagcgac tcagcggata gccatcattc ggcccgtctt gtatcccgac
     3541 aaacgggctg ctatgggtga tttcttgcgg aggagagaag tcgttggatc ccgatgctcg
     3601 ccaacccagc gatccagcgg ccgcgccgtc gacccagttg gtgctggaac tctacaaagc
     3661 ttacctgacg gacctcggaa acatcggcag ccgctacacc acctacaacg gattctatgt
     3721 ttccatcgtc tcgggactgc tcgcggttct ggcccttacg aaacgcggcg aagcgctcga
     3781 gaaattcgat actcttttgt acctcgtcat tcccattttc gctggtctgc tctgcctggt
     3841 ctggcgggag acgatgacct ttcatagcgc gctcttccgg accaagttcg atgtcttgcg
     3901 cgagatggaa gaatatctcc cattcaaggc atatacgaga gaggaaaaat tgcggaggcc
     3961 cggccgccgg ttgatcaaga atgatgagtt gataccggtc attctcgcac ttcccttcgc
     4021 ggtggtcttt attttcggtc tgtggaaact gttacagcgg tgacggctga agcggctctc
     4081 gagcaagtgg aatcgtaaca tcgtcattcc cgacggtgat gggctgctga tgctcacccc
     4141 tcaccctccc gttgctgcgc tccttcggcc ggctcaggag ggcccctccc tctcccacaa
     4201 ggggagaggg tcagaattgg tcgaggtcga actccaggac ggtgctgccg cccgcgacgg
     4261 cagggagcag cgacggtgcg gttgccagca catgctcggt gtagaactgc gcgctgatga
     4321 gcttcgcgcg atgaaacggc gcctggccgt tgccctgcgc cagagcgcgc tctgaggcga
     4381 gcgcgccgcg cgccatgagc cagccgccgg tgacgttgcc catgagcttg aggtaaggca
     4441 cggcgccggc gagcacgcgg gcgggctcgg ccgttccgtg ctcaaccagc catcgcgtgg
     4501 cgacgtcgag cgcgtcgatc gcggccttga gcggcgcgcg gatcgccgcg agcgcagcgc
     4561 cgggcacgtt cgccagcgcg ccgtccgtcg cgcgcatctc ggcgatgagc tcgcgcacgg
     4621 cgaggccgct gtcgcgcagc accttgcggc cgacgaggtc gttggcctgg atgccgttgg
     4681 tgccttcgta gatcggcaag atccgcgcgt cgcgcagatg ctgcgtcgca cccgtctcct
     4741 cgatgaagcc catgccgcca tggacctgca cgccggtcga cgcgatggcg acgccgagat
     4801 cggtgcacca cgccttcacc accggcgtca gcaggtcgac gcggcgctgc tggagcgcgc
     4861 gcgtctcggt gtcgggatga tgccgcgccc ggtcgacggc gcccgccgta taatatgaga
     4921 gcgcgcgcat cgcctcggtg tcggcgcgca tcgccagcag catgcgccgg acatcggcgt
     4981 gatggatgat cggcggcgtg ccggagccgg tcgcgccgac gggcttgccc tggacgcgct
     5041 gccgggcgaa atcgcgcgcc tgctgatagg cgcgctcggc gatggcgacg ccctgaagcc
     5101 cgacattgag ccgcgcattg ttcatcatcg tgaacatgca ttccatgccg cggttctctt
     5161 cgccgacgag gaagcccgtg gcgccgccgg catcgccata agcgaggacg caggtcgggc
     5221 tcgcatgaat gccgagcttg tgctcgaggc tgacgcagcg cacgtcgttg cgcgcgccga
     5281 ggctgccgtc gctgttcacc aggaatttcg gcacgaggaa cagcgagatg ccgcgcgtgc
     5341 cggcgggcgc gccgggaagc cgcgccagca cgagatggac gatgttctcg gccatgtcgt
     5401 ggtcgccata ggtgatgaag atcttctgcc cggtgatgcg gtaatggtcg cccgcacgct
     5461 ccgcccgcgt cctgagcgcg ccgacatcgg agcccgcttg cggctccgtc aggttcatcg
     5521 tgccggtcca ttcgccggag acgagcttcg gcaaatagag acgttgttgc tcgggcgtgc
     5581 catgcgccgc cagcgcctcg acggcgccga tggtgaggag cgggcaaagg gcaaagctca
     5641 tattggccgc gttccacatc tcggcgacgg ccgtggcgag cgcgaagggc aggccttgcc
     5701 cgccatgctc cgtggggaag gggagggcat tccagccgcc cgccacatgc tcggcccagg
     5761 cttccttgaa gcctgggggc gtgcgcacga cgccgttttc gagccggctc ggctggcggt
     5821 cgccaatgcg gttgagcggc gcgatcacgc ccgcggcgag cttgccggct tcctcgagga
     5881 tggtatcgac gagatcggcg ctcgcctgct cgcagcccgg tagcgcggcg atctcggcaa
     5941 tgccggcgat ctcgttgagc gcgaagcgca tatcggcgag cggggcggcg tagtcggtca
     6001 cggctgggtt ccctgttggg ggcccgcgcc ggcgcgggcc ggaaacgcgc atgccataac
     6061 ccgccgggag cgaccgaagc aagcctgggc ggcgccggcg gtgcggagtc gtcagacgac
     6121 tctttcatcg ttccttcttc tcttctgtgt cgcggaacgc ggtggtgcat gccggaagat
     6181 gcagcccgca agcgctggcg tcgggccgcg acgtcgcctt atcatgccgc tcttcgccag
     6241 gccggaggct cgaggcgccg atgcgcaaat cttggtggga cggcgggtat cgttggcagc
     6301 gcctcgcgct gctggcggcg accgccttca ggcatggcgc gcctctcgcc ttcgatctgc
     6361 tgcgcggccg tccggtccgc caggaggatg tcg
//
LOCUS       DASYNS010000544         4710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_138948, whole genome shotgun sequence.
ACCESSION   DASYNS010000544 DASYNS010000000
VERSION     DASYNS010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138948"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..3719)
                     /locus_tag="VGP50_16745"
     CDS             complement(216..3719)
                     /locus_tag="VGP50_16745"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8029084.1"
                     /translation="MRVVDERSFSFAGFTLDLRRGSLRRDEREIELRAKSFAVLRYFV
                     ENAGRLMPRDELMTAIWRDVVVTDESLTHCVSDIRCALGDVDQRIIKTVLGRGYTFLA
                     PVTELGAAAPNGHGSPRLVLPPESGHTDRVPLERRHPERRQLTILFCDLVGSTALSGR
                     LDPEDLHDLMSAYHCACAAEITKAGGFAAEQSGDAVIAYFGYPLAQEDDAERAIRAAL
                     GLRDTIGALVRARGSALQSRIGIATGLVVVGDIIGTGDVRVAGEAPVIASRLQGLAAP
                     DRIVIADGTRRLAGDVFEYRDLGAVMLRGRAEPVQAWEVSGTSALRSRFAAKRGARLT
                     PLVSREEEMELLQRRWRQAKSGMGRVMLIAGEPGIGKSRLIVELGERLASEPHTALQY
                     FCSPDLVDSPFHPVVAQLERTAGFDRYDGTAAKLDKLATLLGGAAPDGSDLPLLAELL
                     SIPTGDRFVPRGWSPQRKKRETLEALLRQFERASRHGPVLAVVEDVHWIDPSSLELLD
                     MMVERVADLPVLLVVTYRPEFQPPWVDASHATLLRLSRLSQQDSAALAALVAGDGALP
                     ADILAAIAAHSDGIPIFVEELTKTVEEARGCDEARRSAAAVPALTIPATLRASLMARL
                     DRLGPAANEVADIAAVIGREFSHELLEPVAQKGEEDLKSALARLRDAGLVYCRGTPPR
                     ATYTFKHALVRDAAYERLLRGPRRSLHARIASTLEKRFPETGAQKPELLALHWTEAGS
                     IGKAIGYWVEAARLARSRHALQEAVALARKGLALLSGREDGPERWRAELQLHSDLGWA
                     LFHWKGEEAPEAGAALSRGRALCVLLGDKPRLGPMLYAEASHHIACARFIDGRRMAEQ
                     LLCVALECNDVGLEATAHETLGRSYHWCGAYGSAIAHFEHALRDPAAGARDQFRLARD
                     GRAISLAYLAADLACLGYLDRAAALRDQALALARQAHPHTLAIALLFARGVDLDRGKL
                     QAALECRTAFEALTREQSFTFHTATVDLSRAVVSSVRGETEEGLSLARRALADPAMVN
                     RMSGQTLRLLLLAGCCERAGRVDEALSLLDTGLEKAATTGERCNEPELHRLRGAWLAA
                     HRPERRAEAEECHRQAIAVARAQQAKLYELRAATGLARLWRGDGRHGEARDLLAPIYG
                     WFTEGLDAPDLQTARSLLDTL"
     gene            3913..>4710
                     /locus_tag="VGP50_16750"
     CDS             3913..>4710
                     /locus_tag="VGP50_16750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEV8029085.1"
                     /translation="MDLARKHLTRAALLGCIGLSVLVPTAGRAFGPINIVTFAQTNLV
                     SNVPGVAPVTDPALIDPRGMAFFGVGFFWMADAGNGTASLYGGGGQTFPDSPTIPGPA
                     AAPAGSQSRPSGIVANLEGPFDLSFNGTLMPSNFIFVTENGTIDGWNQSEFGTAVLAV
                     DNSAAGALYKGVAIAQANTTTFTSPGFTTSAFTSIYATNFHTGTIDMFGSFFEPVTAT
                     GGFVDNQIPPGFAPFGIREINGELWVTYALQDATKTTDVPGVGNGFID"
BASE COUNT          764 a         1693 c         1494 g          759 t
ORIGIN      
        1 ctcgacacgc aaacgccgac gccgaatatg ttgagagacc ccctcaccct accctctccc
       61 ccgcaagagc gggtggagag ggaaaggttt gggatgtcgg cagcgcatcg cgcagccgct
      121 cgaacccagg cgccgataca ctgacacacg accatcttga tcgggctcgt gtggagagcg
      181 atccccgtcg cgccgtcgcg cccttcgcgg cggacctata gcgtatcgag cagcgacctc
      241 gccgtttgca ggtccggcgc gtcgagaccc tccgtgaacc agccatagat tggcgcgagc
      301 aggtcccgcg cttcgccgtg cctgccatcg ccgcgccaca ggcgagcgag gccggtggcc
      361 gcccgcagct cgtacaattt cgcttgctgc gcgcgggcga ccgcgatcgc ttggcgatgg
      421 cattcctctg cctcggcgcg tcgttccggg cgatgagccg cgagccacgc acccctgagc
      481 cgatgcagct cgggctcgtt gcagcgctcg ccggtggtcg ccgccttctc caagccggtg
      541 tcgagaaggc tcagcgcctc gtcgacccgg cccgcccttt cgcagcatcc ggcaagcagc
      601 agcagccgca aggtttgccc gctcatcctg ttgaccatgg ccggatcggc caaagcccgc
      661 cgcgccagcg acagcccctc ctcggtttcg ccccgtaccg acgacacgac ggcgcgactg
      721 agatcgaccg tggccgtgtg gaaggtgaag ctctgctctc gcgtcagtgc ctcgaacgcc
      781 gtccggcatt cgagagcggc ctgcagcttg ccgcggtcca aatcgacgcc gcgcgcaaaa
      841 agcagtgcaa tcgccaaggt gtgagggtgc gcttgcctcg ccaaggccag cgcctggtcg
      901 cgcagcgccg ccgctcgatc gagatagccg aggcatgcca ggtcggccgc caaataagcg
      961 agcgaaatgg cgcggccgtc gcgtgcgagt cggaactgat cgcgcgcccc ggcggcgggg
     1021 tcgcgcagcg catgctcgaa atgcgcgatg gccgaaccat acgcgccgca ccaatggtag
     1081 cttcgcccga gcgtctcgtg cgcggtggcc tcgaggccga catcattgca ttcgagagcg
     1141 acgcacagca gctgttccgc cattcgacgt ccgtcgatga accgcgcgca ggcgatgtga
     1201 tggctcgcct ccgcgtagag catcgggccg agccttggct tgtcgccaag aagaacgcag
     1261 agcgcccggc cgcggctgag tgccgctccg gcctctggcg cttcttcgcc cttccagtga
     1321 aacagcgccc atccgaggtc cgagtgcagc tgaagctccg cgcgccatcg ctcgggaccg
     1381 tcctcgcggc ccgagagcag ggccagaccc ttccgagcca gcgcaaccgc ctcctgcagc
     1441 gcgtgccggc tgcgcgccag ccgcgctgct tcgacccaat aaccaatcgc cttgccgatc
     1501 gagcccgctt cggtccaatg cagcgccaaa agctccggct tctgcgcgcc ggtctcggga
     1561 aaccgcttct cgagtgtcga cgcgatgcgc gcgtgaagtg atcgacgtgg gccacgcagc
     1621 aagcgctcat aggccgcgtc gcgcaccaac gcgtgcttga acgtgtacgt cgcccgcggc
     1681 ggcgtccccc ggcaatagac gagaccggcg tcgcggagcc gcgcgagcgc cgatttcagg
     1741 tcctcctcgc ccttttgcgc gaccggttcc agcagctcat gcgagaactc gcggccgatg
     1801 acggcggcga tgtccgcgac ctcgttcgcg gccgggccca ggcggtcgag ccgcgccatc
     1861 aacgacgccc gcagcgtcgc gggaatcgtg agcgcgggca cggcggcggc gctcctacgc
     1921 gcctcatcgc aacccctcgc ctcctctact gtcttggtca gctcctccac gaagatcgga
     1981 atgccgtcgc tgtgcgcggc gatcgcggca aggatgtccg ccggcaatgc gccgtcgccg
     2041 gcgaccaagg cggccaaggc ggcgctgtcc tgttggctga ggcgggacag ccggagcagc
     2101 gtggcatgcg acgcgtcgac ccatggcggc tggaactccg gccggtaggt gacgaccagc
     2161 aggacgggta gatccgccac gcgctcgacc atcatgtcga gcagctcgag cgagctggga
     2221 tcgatccagt gaacgtcctc gacgacggcc agcaccggtc cgtggcggct cgcccgctcg
     2281 aattgtcgca gcagtgcctc gagcgtctct ctcttcttgc gctgcgggct ccagccgcgg
     2341 ggaacgaaac ggtcgccggt cgggatggac aacagttccg ccagaagcgg aaggtcgctt
     2401 ccgtccggcg ccgcgccacc gaggagggtg gcgagcttgt cgagtttcgc cgccgtcccg
     2461 tcgtatcggt cgaagccggc tgtccgctcg agctgtgcga cgaccggatg aaatgggctg
     2521 tccacgaggt cgggtgagca gaaatattga agcgccgtgt gcggctccga ggccagccgc
     2581 tcgccgagct cgacgatgag ccgcgacttg ccgatgcccg gttctccggc gatgagcatc
     2641 acccgcccca tgccgctctt cgcctggcgc catcgccgct gcagcagctc catctcctcc
     2701 tcgcggctca ccaggggcgt gagcctggcg ccgcgcttcg ccgcgaagcg gcttcgcagc
     2761 gcgctggtgc ccgagacttc ccaggcttgc acgggctcgg cgcggccgcg cagcatcacc
     2821 gcgccgagat cgcggtattc gaacacgtcg ccggcgaggc ggcgtgtgcc gtccgcgatc
     2881 acgatccgat ccggcgcagc gaggccttgc agccgcgacg cgatgaccgg cgcttcaccg
     2941 gcgacccgga cgtcaccggt gccgatgatg tcgccgacca ccaccagccc cgtggcgatg
     3001 ccgatccgac tctgaagcgc gctgccccgc gcccgcacaa gcgcgccgat cgtgtcgcgg
     3061 agtccgagcg ccgcgcggat cgcccgctcg gcatcgtcct cctgcgccag cggataaccg
     3121 aaataggcga tgacggcatc gcccgactgc tccgccgcaa agccgcccgc cttggtgatc
     3181 tcggcggcgc aggcgcagtg ataggcgctc atcaggtcat gcagatcctc gggatcgaga
     3241 cgacccgaga gcgccgtcga gccgaccaga tcgcagaaga ggatggtgag ctggcggcgt
     3301 tcggggtggc gccgctcgag cggcactcga tcggtgtgtc cgctctctgg cggtaacacg
     3361 agtctgggcg agccatgccc gttcggcgct gcggcgccaa gctccgtcac cggcgcgagg
     3421 aacgtgtagc cgcgcccgag caccgtcttg atgatgcgct gatcgacgtc gccaagcgcg
     3481 caacggatat cgctcacgca atgcgtcagc gattcatccg tcaccaccac gtcgcgccag
     3541 atcgcggtca tcagctcgtc ccggggcatc agccttccgg cgttctcgac gaagtagcgc
     3601 agcacggcga agctcttcgc cctgagctcg atctcccgtt cgtcgcggcg cagagagccg
     3661 cgccgcaggt cgagggtaaa tcctgcgaag gaaaaggatc tctcgtcgac gacccgcatc
     3721 gtcgctggcc tcgatggcag gcggcgggaa ccgctgttcg tcgatcctac tcgccttttc
     3781 agcgaaattt cctcccgctt tcaggacaaa aatagggcag acccctccaa tagatcattc
     3841 cggtccgctc gtcatgctcg accggccggc ggactggcac gttcaaacga tttcgatagg
     3901 gagaagcgtg aaatggacct tgctcgcaag catcttaccc gtgcagcgct ccttggctgc
     3961 atcgggctat ccgtccttgt cccgacggcc ggccgcgcct tcggcccgat caacatcgtc
     4021 accttcgcgc aaaccaatct ggtgtccaat gtccccggcg tcgcgcccgt caccgatccc
     4081 gctttgatcg acccgcgggg catggccttc ttcggcgtcg gcttcttctg gatggccgat
     4141 gccggcaacg gcacggccag cctttacggc ggcggcggcc agaccttccc ggacagcccc
     4201 actattccag gcccggccgc cgccccggcc ggctcgcaat cccggccctc cggaatagtg
     4261 gccaatctcg agggcccttt cgacttgtcc ttcaacggca cgctcatgcc gtcgaacttc
     4321 atcttcgtca ccgagaacgg caccatcgac ggctggaatc agagcgagtt cgggaccgcc
     4381 gtcctcgccg tcgacaacag tgccgcgggc gcgctctata agggcgtcgc catcgcccaa
     4441 gccaacacga cgaccttcac ctctccgggc ttcaccacct cggccttcac cagcatctat
     4501 gcgaccaatt tccataccgg aacgatcgac atgttcggtt ccttcttcga gccggtgacg
     4561 gccaccggcg gcttcgtcga caaccagatc ccgcccggct tcgcgccctt cggcatccgc
     4621 gagatcaatg gcgagctctg ggtgacctat gcgctccagg acgcgaccaa gaccacggat
     4681 gttccgggcg tcggcaatgg cttcatcgac
//
LOCUS       DASYNS010000545         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4039101, whole genome shotgun sequence.
ACCESSION   DASYNS010000545 DASYNS010000000
VERSION     DASYNS010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4039101"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..89)
                     /locus_tag="VGP50_16755"
     tRNA            complement(3..89)
                     /locus_tag="VGP50_16755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53..55),aa:Leu,seq:caa)
     gene            182..634
                     /locus_tag="VGP50_16760"
     CDS             182..634
                     /locus_tag="VGP50_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV8029086.1"
                     /translation="MAGRYLEDFHPGEVIELGSRTVSAEEIIAFARQFDPQYFHIDPE
                     RAKASPFGGLIASGWHTVAMYMRLVVDRFISGMAESMGSPGVDGIRWLKPVRPGDTLM
                     GRSTVVEIIPSKSRADRGTLKSLGELVNQHGEVVMQMNSVGFFARRPK"
     gene            706..1338
                     /locus_tag="VGP50_16765"
     CDS             706..1338
                     /locus_tag="VGP50_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEV8029087.1"
                     /translation="MLDRIIAPLAAVIIGTISSMGYVGIVLLMAIESACIPLPSEIIM
                     PFSGYLVSTGRFELWLVATAGALGCNVGSTIAYAVGYWGGRPLVEKWGGYVLMSRRDL
                     ALVDRFFARFGGITVFVCRLLPVVRTFIALPAGIARMPQAKFQIYTFLGSWPWCFALA
                     YIGAKLGERWDSDPELRNVMHRFDFVIVAVLVLGIVWYVRRHWQHRLSKE"
     gene            1546..2040
                     /locus_tag="VGP50_16770"
     CDS             1546..2040
                     /locus_tag="VGP50_16770"
                     /inference="COORDINATES: protein motif:HMM:NF014639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEV8029088.1"
                     /translation="MTAPRLAPFLVLVASVALVGGALLFQYVGHLAPCELCLYQRWPY
                     YAAIPLSLVAVLAGRRPATLGLLALCALLFVAGGGLAFYHVGVEQHWFAGPTACTDSL
                     STSGSVEDFTARLLATQPVRCDDIAWSLFGVSLAGWNFLASLGLVVFCGAALRALARS
                     PRLA"
     gene            2037..>2554
                     /locus_tag="VGP50_16775"
     CDS             2037..>2554
                     /locus_tag="VGP50_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEV8029089.1"
                     /translation="MTAPSPRLPGDPPRAEGIARMIRVDHAGEYGAARIYEGQLAVLG
                     RSPSMPVIRRMAEQEKRHLAAFDRLVVERHVRPTLLSPLWHVAGFALGAATALLGERA
                     AMACTVAVEEVIDAHYAQQIATLGAEEPELKTTLTEFRGDEIEHRDTGLAHGAAEAPA
                     YALLSTAIKTGSR"
BASE COUNT          349 a          871 c          869 g          465 t
ORIGIN      
        1 gatggtgccc cccgtcggaa tcgaaccgac atgatcttgc gatcgcggga ttttgagtcc
       61 cgtgcgtcta ccagttccgc cagaggggca cgggcgcagt ggtagctttc gcctggacgc
      121 gatgcaagag cgccgtccga ggcgcttgcc cgtgcgggca atcccggcta gacccgtgcc
      181 catggcggga cgctatcttg aggacttcca tccgggcgag gtgatcgagc tcggcagccg
      241 tacggtcagc gccgaggaga tcatcgcctt cgcccggcaa tttgatccgc aatatttcca
      301 catcgatccc gagcgcgcga aggcaagccc gttcggcggc ctcatcgcca gcggctggca
      361 tacggtcgca atgtatatgc gcctggtcgt cgaccgcttc atcagcggca tggccgagag
      421 catgggctcg ccgggcgtcg acggcattcg ctggctgaag ccggtgcggc cgggcgacac
      481 gctgatggga cgctccaccg tcgtcgagat cattccgtcg aagagccgcg ccgaccgcgg
      541 cacgctcaag tcgctgggcg aactggtcaa ccagcatggc gaggtcgtca tgcagatgaa
      601 cagcgtcggc ttcttcgcgc gccggccgaa atgatgcgcg cgccgccatt gccgcgcacc
      661 ggagaacgcg gcgagtaacg ccgcgggatc gccagggagc cgcccttgct cgatcggatc
      721 atcgcgccgc tcgccgccgt catcattggg accatctcgt cgatgggata tgtcggcatc
      781 gtgctgctga tggcgatcga atccgcctgc attccgctgc cttccgagat catcatgccg
      841 ttctcgggtt atctcgtctc gacggggcgc ttcgagctgt ggctggtggc gacggcgggc
      901 gcgctcggct gcaatgtcgg ctcgacgatc gcctatgccg tgggctattg gggcgggcgg
      961 ccgctggtgg agaaatgggg cggctacgtc ctgatgagcc gccgcgatct cgcgctggtc
     1021 gaccgcttct tcgcgcgctt cggcggcatc accgtcttcg tctgccggct cttgccggtg
     1081 gtgcgcacct tcatcgcgct gcccgccggc atcgcgcgca tgccgcaggc caagttccag
     1141 atctacacct ttctcggttc ctggccctgg tgcttcgcgc tcgcttacat cggcgccaag
     1201 ctcggcgagc gctgggacag cgatcccgaa ctgcgcaatg tcatgcaccg cttcgatttc
     1261 gtgatcgtcg ccgttctcgt cctcggcatc gtctggtatg tccgccggca ttggcagcat
     1321 cggctctcga aggagtaaga tggcaatgaa ccggccgggc gatacatcgc gtcctcgccg
     1381 taggccgcaa gggcagggtt ggcgcggatg ggattcacca cggagacacg gaggacacgg
     1441 agaagcgaag aatggcgcgc ttcgcgcgcc caatccgtgt cctccgtgtc tccgtggtca
     1501 atctttccca tcggcgtttc ggccggacgc gcgcgtggag ctgccatgac cgcgccgcgc
     1561 ctcgcgccct tcctcgtcct cgtcgccagc gtggcgctgg tggggggcgc gctgctgttt
     1621 cagtatgtcg gccacctggc gccgtgcgag ctctgcctct atcagcgctg gccctattat
     1681 gctgcgatcc cgttgtcgct cgtggcggtg ctcgccggac ggcggccggc gacgctcggg
     1741 ctgctggcgc tgtgcgccct gctgttcgtc gccggcggag gcctcgcctt ttaccatgtc
     1801 ggcgtcgagc aacattggtt cgccggcccg acggcctgca ccgacagcct ctccacgagc
     1861 gggagcgtcg aggacttcac ggcgcgtctg ctggcgaccc aaccggtgcg ctgcgacgac
     1921 atcgcgtggt cgctcttcgg cgtctcgctc gccggttgga acttcctggc gtcgctcggg
     1981 ctcgtcgtct tctgcggcgc cgcgctgcgg gcgctcgcgc gatcgccgag gctcgcatga
     2041 ccgcgccgtc gccgcgcctg ccgggggatc cgccgcgcgc ggaagggatc gcgcgcatga
     2101 tccgggtcga tcacgcgggc gagtatggcg ccgcgcgcat ctatgagggg cagctcgccg
     2161 tgctgggacg ctcgccctcc atgccggtga tccgccgcat ggccgagcag gagaagcgcc
     2221 acctcgccgc cttcgaccgg ctggtcgtcg agcgccacgt gcggccgacc ttgctgtcgc
     2281 cgctctggca tgtggcgggc ttcgcgctgg gcgccgcgac ggcgctcctg ggcgagcgcg
     2341 ctgccatggc ctgcaccgtc gcggtcgagg aagtgatcga cgcccattat gcgcaacaga
     2401 tcgcgacgct cggcgccgag gagcccgaac tcaaaacgac gctcaccgag tttcgcggcg
     2461 acgagatcga gcaccgcgac acgggccttg cccatggcgc cgccgaggcg ccggcctatg
     2521 cgctgctgag caccgccatc aagacgggct cgcg
//
LOCUS       DASYNS010000546         3728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_139139, whole genome shotgun sequence.
ACCESSION   DASYNS010000546 DASYNS010000000
VERSION     DASYNS010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139139"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VGP50_16780"
     CDS             <1..472
                     /locus_tag="VGP50_16780"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV8029090.1"
                     /translation="ASIFLALPGRPRLLQQGALGMPSRNLEAWMWAEACEALARAERL
                     NRQFFRLGTRETVPTWEPPVDVLETEDELWIIVALPGVEASQVEIGVSERDLVIAGVR
                     SAPLRRSAAIHRLEIPQGRFERSIPLPPGRFELMRRDLAHGCLTLVLRKDSRSR"
     gene            478..2856
                     /gene="lon"
                     /locus_tag="VGP50_16785"
     CDS             478..2856
                     /gene="lon"
                     /locus_tag="VGP50_16785"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEV8029091.1"
                     /translation="MAADDPSAAATQGDLPPLPGDALIIIPVRGAVLFPGAVLPITIG
                     RQRSVAAVQEAVRRELPVGLLLQRDPKVEEPAAADLYPVGTEAGVVRYLTAPDGSHHM
                     ICQGQHRFRLIEILSGYPFLVARVERVGEAELRTPELEARLLYLRRQAHEALELLPQA
                     PPELAAAIDGINAGGQLADLIANLMDLEITEKQSVLETFDVLLRMKRVSDLLAHRIEV
                     LRLSRQIGQQTKETVEGRQREFLLREQLKTIQKELGEDDAKSEEIAELTRRIAEAKMP
                     PEVEAQAKKELVRLERMPEGAAEYSMVRTYLDWLVELPWSVTTEHIIDIGEARRVLDE
                     DHFDLAKVKRRILEYLAVQKLKPGGKSPILCFVGPPGVGKTSLGQSIARATGRKFVRV
                     SLGGVHDEAEIRGHRRTYIGALPGNIIQGIRKAGAHDCVMMLDEIDKLGAGIQGDPSA
                     ALLEVLDPEQNSTFRDGYLGVPFDLSQVMFIGTANVIDNVPGPLRDRMEVLELAGYTE
                     EEKLQIARRYLVTRQLEAAGLDLERVTITDDAIRTIIRDYTREAGVRNLERAIGSTFR
                     HSAMRFAEGQTEPIHIDVEEVPAILGPQRFESEVAMRTSIPGVATGLAWTPAGGDILF
                     IEATRIPGNGRLILTGQLGDVMKESAQAALSLVKSRARSLGIDEELFARSDIHVHVPA
                     GAIPKDGPSAGVAMTVALVSLLTNRATRSDTAMTGEISLRGLVLPVGGIKEKVTAAVR
                     AGITTLLLPARNRRDFEDIPEEARRKARFVWCERIDDVVAEAIAQEAMAAAQ"
     gene            2874..3566
                     /locus_tag="VGP50_16790"
     CDS             2874..3566
                     /locus_tag="VGP50_16790"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV8029092.1"
                     /translation="MADASSGAGDRATLSFASAGKSIGVDWHRAARPGIRPAVLMLHG
                     RDGPDRFADAYRFAARELSERGYHVLFVHYFDASTDDTLFGGAGLANFFAWMRVVEDC
                     VGWAGSQADVDPERVAVLGVSLGASIALAQASQDRRIKAVIDFYGMLPLPALAMTHRM
                     PATLILHGAQDWVVHVAAAYQLDAFLKTIGATHETHIYPDAGHGFRDTVGADAVRRTI
                     AFLDRHVGEHAG"
BASE COUNT          607 a         1301 c         1240 g          580 t
ORIGIN      
        1 ggcgagcatc tttctggccc tgccaggccg gccgcgactg cttcaacaag gcgccttggg
       61 tatgccatct cgtaacctcg aagcctggat gtgggctgaa gcctgtgagg cgctggcacg
      121 cgccgagcgg ctgaaccggc agtttttccg cctcggcacg cgcgaaaccg tcccgacctg
      181 ggagccgccc gtcgacgtgc tcgagaccga ggacgaactc tggatcatcg tggcactgcc
      241 cggcgtcgag gcatcgcagg tcgagatcgg cgtctccgag cgggatctcg tgatcgccgg
      301 ggtgcgctcg gcgccgctcc gccggagcgc ggcgattcac cgactggaga tcccgcaggg
      361 gcggttcgag cgcagcatcc cccttccccc cggtcgattc gagctgatgc gccgcgacct
      421 cgctcatggc tgcctcaccc tggtgctgcg caaagacagc agatcacgat agaaacaatg
      481 gccgccgacg atccctccgc cgccgcgacg cagggcgatc tgccgcccct ccccggcgat
      541 gccctgatca tcatccctgt gcgaggcgcc gtcctgtttc cgggcgccgt gctgccgatc
      601 acgatcggcc gccagcgctc tgtcgccgcg gtgcaagaag cggtgcgccg ggagctgccg
      661 gtcggcctgc tgttgcagcg cgaccccaag gtcgaggagc ccgccgccgc cgatctctac
      721 ccggtcggca cggaggctgg cgtggtgcgc tatctgacgg cacccgacgg ctcgcatcac
      781 atgatctgcc aggggcagca tcgcttccgc ctcattgaga ttctgtcggg gtatcctttc
      841 ctggtggcgc gcgtggagcg ggtcggcgag gccgagctgc gcacgccgga gctcgaggcg
      901 cggctgctct atctgcgccg ccaggcgcat gaggcgctcg agctcttgcc ccaagcaccg
      961 ccggagctcg cggccgccat cgacggcatc aatgccggcg gacagctcgc cgatctcatc
     1021 gccaatctca tggatctcga gatcaccgag aagcaaagcg tgctcgagac cttcgacgtg
     1081 ctgctgcgca tgaagcgcgt ctccgatctg ctcgcccatc gcatcgaggt gctgcgcctc
     1141 tcgcgccaga tcggccagca gaccaaggag acggtggagg gccggcagcg cgagttcctg
     1201 ctgcgcgagc agctcaagac gatccagaag gagctcggcg aggacgacgc caagagcgag
     1261 gagatcgccg agctcacgcg ccgcatcgcc gaagcgaaga tgccgcccga ggtcgaagca
     1321 caggccaaga aggagctggt gcggctcgaa cgcatgccgg aaggcgcggc cgaatattcg
     1381 atggtgcgga cctatctcga ctggctggtc gagctgccct ggtcggtgac gaccgagcac
     1441 atcatcgaca tcggcgaagc ccggcgcgtg ctcgacgagg accatttcga cctcgccaag
     1501 gtgaagcggc gcatcctcga atacctcgcc gtgcagaagc tgaagccggg tgggaagagc
     1561 ccgatcctgt gtttcgtcgg gccgcccggc gtgggcaaga cctcgctggg ccagagcatc
     1621 gcccgcgcca cggggcgcaa attcgtgcgc gtgagcctgg gcggcgtcca tgacgaggcc
     1681 gagatccgcg gccatcgccg gacctatatc ggcgcgctgc ccggcaacat catccaaggc
     1741 atccgcaagg ccggcgcgca tgactgcgtc atgatgctgg acgagatcga caagctcggc
     1801 gccggcatcc agggcgatcc ctcggcggcg ctgctcgagg tgctcgaccc tgagcagaac
     1861 tcgaccttcc gcgacggcta tctcggcgtg cccttcgatc tctcccaggt gatgttcatc
     1921 ggcaccgcca atgtcatcga caatgtgccc ggccccttgc gcgaccgcat ggaggtgctg
     1981 gagctcgccg ggtataccga ggaagagaag ctgcagatcg cgcggcgcta cctcgtgacg
     2041 cgccagctcg aagccgccgg cctcgacctc gagcgcgtca ccatcaccga cgacgccatc
     2101 cgcacgatca tccgcgacta cacgcgggag gccggcgtgc gcaacctcga gcgcgccatc
     2161 ggctcgacgt tccgccactc cgccatgcgc ttcgccgagg gccagacgga gccgatccac
     2221 atcgatgtcg aggaagtccc ggccattctc gggccgcagc gcttcgagag cgaagtggcg
     2281 atgcgcacca gcatcccggg cgtcgcgacc ggccttgcct ggacaccggc cggcggcgac
     2341 atcctcttca tcgaggcgac gcgcatcccg ggcaacggca ggctcatcct caccgggcag
     2401 ctcggcgacg tcatgaagga gagcgcgcag gcagcgctga gcctcgtcaa gtcgcgggcg
     2461 cggtcgctcg gcatcgacga ggagctcttc gccaggagcg acatccatgt gcatgtcccc
     2521 gccggcgcca tccccaagga cgggccctcg gccggcgtcg ccatgacggt ggcgctggtc
     2581 tcactgctca ccaaccgcgc gacgcgcagt gacaccgcca tgaccggcga gatcagcctg
     2641 cgcgggctgg tgctaccggt gggcgggatc aaggagaagg tgacggcggc ggtgcgcgcc
     2701 ggcatcacca ccctcctgct tcccgcgcgc aaccggcgcg acttcgagga catcccggag
     2761 gaggcgcggc gcaaggcgcg cttcgtctgg tgcgagcgca tcgacgacgt cgtcgccgag
     2821 gcgatcgcgc aagaggcgat ggctgcggcc caatagcagc gggaaatcca gcaatggcag
     2881 atgcgtcttc gggcgcggga gaccgcgcca ccctttcctt cgcgagcgcc ggcaaatcaa
     2941 tcggggtcga ctggcaccgc gcggcgcggc cgggtatccg gccggcggtg ctgatgctgc
     3001 atgggcgcga cgggcccgac cgcttcgccg acgcctatcg cttcgcggcc cgggagttgt
     3061 ccgagcgcgg ctatcatgtg ctgttcgtcc attatttcga tgccagcacc gacgacacgc
     3121 tcttcggcgg cgccggcctc gccaacttct tcgcctggat gcgggtggtg gaggactgcg
     3181 tgggctgggc gggatcacag gcggacgtcg atcccgaacg cgtcgccgtc ctcggcgtat
     3241 cgctcggggc ctccatcgcc ttggcgcaag caagccagga caggcgcatc aaggccgtca
     3301 tcgatttcta cggcatgctg ccgctgccgg ccctggcgat gacccaccgc atgccggcga
     3361 cgctgatcct ccatggcgcc caggactggg ttgttcacgt cgccgcggcc tatcagctcg
     3421 acgcctttct caagacgatc ggtgcgacgc acgagacaca catttatccc gatgcgggcc
     3481 acggcttccg cgacaccgtg ggcgccgacg cggtgcgccg cacgatcgca ttcctcgacc
     3541 ggcacgtcgg cgagcacgcc gggtgagggc accgcgccgg caagccgcgc tttagacggc
     3601 ctctccccac ccagcgccgc gcgctctttc cgcccgttca tcgggctgaa ctcgcttgct
     3661 ttttaggaat attcagagaa tgcgcggccg gacgacttaa tcgagccgca atcgctttct
     3721 gctgtgat
//
LOCUS       DASYNS010000547        11857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2323194, whole genome shotgun sequence.
ACCESSION   DASYNS010000547 DASYNS010000000
VERSION     DASYNS010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 11857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11857
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2323194"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VGP50_16795"
     CDS             complement(<1..270)
                     /locus_tag="VGP50_16795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV8029093.1"
                     /translation="MTVGASLPENRDGGRRTTLLPYLLLSLAALIWAGNWVVGRGIRD
                     TVPPIALAFWRWAIAALILAPIAVPRLRGRLPLLRRHWRVLFVLSA"
     gene            complement(465..1202)
                     /locus_tag="VGP50_16800"
     CDS             complement(465..1202)
                     /locus_tag="VGP50_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8029094.1"
                     /translation="MQVTKTTDHILVVDDQKEICEVVQQYLSSEGYRVSAAHDGTGMR
                     RVMGQDEVDLVILDLMLPGEDGLTLARALREESTVGIIILTGRGETVDRIIGLEMGAD
                     DYLPKPFHLRELLARVKSVLRRASTRAQNEKAAGVSARARARFAGWHLDLSTRELVSP
                     TGGEVRLTTGEFDLLAAFVNNANQVLSRDRLLDLARNREAGPFDRTIDVQVGRLRRKL
                     EPDPQKPTMIKTVRGTGYIFTPQVEVS"
     gene            1441..2025
                     /locus_tag="VGP50_16805"
     CDS             1441..2025
                     /locus_tag="VGP50_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV8029095.1"
                     /translation="MAEICRKIEEEIPRLRRYARALTRDVNAADDLVQDCLTRALSKV
                     HLWQKGTDLRAWLFTILHNQYVNHVRRAVREGSAVGLSDSEPMLTSAPNQGKRLELRD
                     LERAMAKLPEEQRSVLLLVGLEGMRYEEVAAVLNVPVGTIRSRLSRGREMLRQLMGMV
                     PDDEEPARLPMPRRLPAAKAQAEAAFGRRALPLR"
     gene            2139..2420
                     /locus_tag="VGP50_16810"
     CDS             2139..2420
                     /locus_tag="VGP50_16810"
                     /inference="COORDINATES: protein motif:HMM:NF021471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEV8029096.1"
                     /translation="MLGRHVYRVSPLESGGWSVEKEGEAQPRMKRPSREEATRAACDL
                     AAADAPSKVVIEEQGGAIAAERLFGDDTAAALEREADGRHPHTSAPPRR"
     gene            2673..5969
                     /locus_tag="VGP50_16815"
     CDS             2673..5969
                     /locus_tag="VGP50_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEV8029097.1"
                     /translation="MPRQGLRIYNLFPLLAGTIAEWRAELPRIAAMRFNCVFVNPFHF
                     PGFSGSLYAVKDHYRLNPLLKGDARDSDDALLAGFTAECRRHGLLAMMDLVVNHTARD
                     SELVASHPHWFAREPGGGVRSPSAIDPADATNITVWGDLAEIAYDGGSDEAEIIAYFQ
                     EVIRHYLGLGFRGFRCDAAYKVPPRVWRALIAGARAVAPDCVFCAETLGARLEEVARL
                     DGTGFDYLFNSAKWWDFESPWLLEQYDRFHRIAPSIAFPESHDTERLAAELARSGIVD
                     AEAIAVRHRQAYAFAACFATGVMMPMGFEFGWSRRLDVVKTRRLPVEPKRFDLSGEIA
                     AINAMKQATPALNEEGPQSRLTTPDDPVVALARRTADGREWAFSLFNTDDTTAQEIDA
                     DGLLAAADGHALLLEEVTPGAEPGLGLHLVLEPLAVRVLRGLRPAPARVPVTETPPRI
                     HPEWSANARILIEEVQPELDGGRYPVKRVVGDTLEVQADILRDGHDVLRAAVLYRPED
                     AGEWRQSPMRLFDNDRWVGAVPLEENKRYCYTIEAWTDVFASWRADTAKKRDAGQATE
                     SELEEGRALVVAALPRAEAPDRRLLERLLRDSDAGDSEIRAEIMLSLLLADAMARAGD
                     RSDRTRYRRALEVVVDRPRARFGAWYEMFPRSQGRIPGRSGTFEDAAARLGEIRAMGF
                     DVVYLPPIHPIGRLNRKGRDNSLTVRPGDPGSPYAIGSPEGGHDAVHPELGGIAGFRG
                     FVAAARGLGLDVALDYAIQCAPDHPWVKEHPEWFVFRRDGSIKYAENPPKKYQDIVNV
                     DFYNRDRDGLWQALRDILLFWIDEGVRIFRVDNPHTKPLPFWEWLIGEVQARYPDVIF
                     LSEAFTRPKMMRALAKAGFTQSYTYFTWRVTKEELIAYGTELAQGPAKEFFRPNFFTN
                     TPDILPLHLQQGGRPAFRSRLVLAATMAPAYGIYSGFELCENGAIPGSEEYLHSEKYE
                     YKVWDWDRPGHIKDDVARLNRIRRENPALQELTNLQFYPADDDHVVFYGKMTADGGNR
                     IFVAVNLDPFAVHEATIDLPLAAMRIAEVETFTAVELFTGEEKRWRGARQRLRLDPDV
                     NPAAIFRVER"
     gene            6227..9541
                     /gene="treS"
                     /locus_tag="VGP50_16820"
     CDS             6227..9541
                     /gene="treS"
                     /locus_tag="VGP50_16820"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011631458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEV8029098.1"
                     /translation="MPDTSVATEAAPLVGDPLWYKDAIVYQLHVKAFFDSTDDGIGDF
                     QGLTRKLDYLADLGVSALWLLPFYPSPLRDDGYDIADYRGVNPAYGGLSDFRAFVRAA
                     HTRGMRVITELVINHTSDQHPWFQRARRAKAGSFWRNYYVWSDTDQKFLGTRIIFLDA
                     ESSNWGWDPVAKAYYWHRFYAHQPDLNFDNPRVVREVVNVMRYWLDMGVDGMRLDAVP
                     YLIEREGTNNENLPETHGVLKRLRAELDRRYGDRMLLAEANQWPEDVLQYFGEGDECH
                     MAFHFPLMPRIFMAVAIEDRHPITDIMRQTPDIPANCQWAIFLRNHDELTLEMVTDRE
                     RDYLWKHYATDKRMRLNLGIRRRLAPLMENDRRRMELLNGLMFAMPGTPVMYYGDEIG
                     MGDNIFLGDRDGVRTPMQWSPDRNGGFSRADPAQLYLPPIMDAVYGFQSVNVEAQSRS
                     PSSFLNWMKRLIAVRKARRAFGRGEQHFLYPANRKVLAFLRSAGDETILCVFNLARAP
                     QAVELDLSGFKGRVPVELFGRSFFPPIGDLPYLLTLPGHGFYWFLLDASSAVEPNWRG
                     ASLPEFVTLVMPDGWTGPLDRHNRRLFESEVLPAYLPNQRWFAGKSQRITQAAVAAVV
                     ELPRAGGQSWMLSLIDIAFRDGEQQRYFLPLALTWDEEESELRKATLPYTLAVARRGR
                     REGAVYEATADQDFALALLQAIGAQAELKGPRGRARFERTTAYGLAPPPEKPDVRRVG
                     GEQSNSSLLIDEYGVLKLYRRLAAGIQPEIEMTRFLTEVAHFPHTPPLLGAVHFSDEA
                     GGEAALGVLFAYVRNQGSAWSQSLNYLTRHLDEALLMAPHEARPSEPPTPEALAHPLY
                     LELAAQLGRRTAELHRALCPDVLVDDAFAPEPTTAEDLAHWRERAIGAARVMLDNLAR
                     RRDGLPAPAQALADRLLARRDKVPERFASLLPPDIAAVKTRFHGDYHLGQVLAVQDDF
                     YIIDFEGEPLRAMAERRQKSSPLRDVAGMLRSYAYAAATAVRQMTEIQPAAQPVLQER
                     AEEWRRQVSDAFLTRYRAEMAGVRSIPAESGKAAALLDFFTLEKAMYEVDYELAQRPA
                     WVAIPLAGVLALLEGA"
     gene            9541..11736
                     /gene="glgB"
                     /locus_tag="VGP50_16825"
     CDS             9541..11736
                     /gene="glgB"
                     /locus_tag="VGP50_16825"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV8029099.1"
                     /translation="MATLSPAARAILEGRHGDPFSFLGLHPVGGNLCLRVFLPWPRRV
                     WAIDAATGELAGELNRIHEDGLFSGMLGHRPPFRYRLRAEGALGTIEFDDVYAFPPVL
                     GDLDIHLLAEGTHHESYAVMGAHCREHEGVAGVSFAVWAPNAHRVSVVGDFNEWDGRR
                     YPMRLRHAAGVWELFIPGLEPGHFYKYEILGAEGKVLLKADPYARRAEHPPGTASCIA
                     APAKHAWSDAEWIETRWRRNDRHAPISVYEAHLGSWRRKLEEGGGYLTYRELAEQLVP
                     YVRDMGFTHLELMPVSEYPFDGSWGYQPVSLFAPTSRFGTPDDFRAFVDACHAAGIAL
                     WLDWVPGHFPTDAHGLARFDGTALYEHADPRQGFQPDWNTFIYNFGRREVANFLLSSA
                     RYWIDAFHADGLRVDAVASMLYLDYSRPAGEWIPNEHGGRENLAAVAFFKRMNELIFG
                     TGTGATTAAEESTAWPMVSRPTYLGGLGFGFKWNMGWMHDTLRYMAQDPIYRRYHHND
                     LTFGLLYAFHENFILPLSHDEVVHGKGALIAKMPGDRWQRFANLRAYYGFLWTHPGKK
                     LLFMGGEFAQEREWNHDRGLDWQLLADPMHRGVQMVVRDLNHLYRELPALHALDAEPR
                     GFQWIDGSDADQSTISYIRRSEDPQTLAVVLCNFTPVPRHGYRIGVPQSGLYRERFNS
                     DAIEYGGSGLGNAGAVRTEAHPSHGHAHSLVLTLPPLATLIFAHEPESG"
BASE COUNT         1910 a         4065 c         3952 g         1930 t
ORIGIN      
        1 ggcggaaagc acgaagagca cgcgccagtg ccggcgcagc agcggcaagc ggcctctgag
       61 ccgcggcacg gcgatgggtg cgaggatgag ggccgcgatc gcccagcgcc agaaggccag
      121 agcgatggga ggcacggtgt cgcggatgcc gcggccgacc acccagttgc ccgcccagat
      181 gagagcggcg agcgacagca gtaaataagg tagaagcgtc gtccgccggc cgccgtctcg
      241 gttctctgga agggacgcgc cgacggtcat cggccgcgag agtgcgggcg ggcgggccgc
      301 gcgtcaatgg acggcgccgg aaggctgaca tgcgccgagg ccctctcagc tctccgctgc
      361 cagttgccgc gaaccccggc ggcgcgctcc agcccgcagc gaacaccccg gcattcttcg
      421 cggcgacgac cacgcaagaa tgattctggg gtaagcggga ggcgctagct gacctcgacc
      481 tgcggcgtaa agatatagcc cgtgccgcgc acggtcttga tcatggtcgg cttctgggga
      541 tcgggctcga gcttgcgccg gagccggccg acctggacgt cgatggtgcg gtcgaaagga
      601 cccgcctcgc ggttgcgcgc cagatcgagg agacggtcgc ggctcagcac ctgattggcg
      661 ttgttgacga aggcggccag caggtcgaac tcgcccgtgg tgaggcggac ctcgccgccg
      721 gtcggcgaga cgagctcgcg ggtcgagaga tcgagatgcc agcccgcaaa gcgagcccgg
      781 gcgcgcgcgc tgacgcctgc cgccttctcg ttctgggcgc gagtcgaggc gcgccgcagc
      841 acgctcttca cccgcgccag aagctcacgc aggtgaaagg gcttcggcag atagtcatcg
      901 gcgcccattt cgaggccgat gatgcggtcc accgtctctc cgcgaccggt caaaatgatg
      961 ataccgacgg tcgattcttc gcgcagagcg cgagcgaggg tgagcccgtc ctcgccgggc
     1021 agcatgaggt cgagaatgac gaggtcgacc tcgtcctggc ccatgacgcg gcgcatgccg
     1081 gtaccgtcat gggccgcgct gacccggtac ccctcgctcg acaaatactg ttggacgacc
     1141 tcgcatatct ccttttggtc gtccaccaca aggatatggt ctgtcgtttt tgtgacctgc
     1201 atggcttggc taaatttggc gcatctgtgg cgtcgatgca atatcgcaac ggtaggtccg
     1261 gctctccggg ggatcgcctc tcacgcgcgc agcaaaagct tacacatcgt tacaatttat
     1321 tacccaattc cggcagccct gacatcatgc aattacagga ccccgctaca gtccgtcgtg
     1381 tcgccgcttt gacgttgaac ccgcggacct tttcagcggc gcaggcgaag ggagcatagg
     1441 atggcggaga tctgtcggaa aatcgaggaa gaaatcccgc ggctgcggcg ctacgcgaga
     1501 gcgctcacgc gcgatgtcaa cgccgccgat gatttggtcc aggattgcct gacgcgggcg
     1561 ctcagcaaag ttcatctgtg gcagaagggc acggacctcc gggcgtggct cttcactatt
     1621 ctgcacaatc aatacgtcaa ccacgtccgc cgcgcggtgc gcgaagggtc ggccgtaggg
     1681 ctttccgaca gcgagcccat gctcacctcg gcccccaatc agggtaagcg cctcgagctg
     1741 cgtgatctcg agcgcgccat ggccaagctg ccggaggagc aacgctcggt gctcctcctc
     1801 gtcggcctcg aagggatgcg ttacgaagag gtggcggcgg tgctcaacgt ccccgtcggc
     1861 accatccgct cgcgcctctc gcgcggccgc gaaatgctgc gccagctcat gggcatggtc
     1921 cccgatgacg aagagcccgc gcgcctgccc atgccgcgcc ggcttccggc ggccaaggcg
     1981 caggccgaag ccgcgttcgg tcggcgcgct ctgccattgc gctgacccgg gccgcctcac
     2041 catcctgttg tctcgctcgg gcgcggcccg cgtttcggaa cgcggtcccc cgggcgggcg
     2101 ttgccagcca tagaggccaa gagcgagatg gaggccgcat gctgggacgc catgtctatc
     2161 gcgtgagccc gctcgagagc ggtggctgga gcgttgagaa agagggcgag gcacagccgc
     2221 ggatgaagcg cccgagccgc gaggaagcga cccgcgcggc ctgcgacctc gccgccgcgg
     2281 acgcgcccag caaggtcgtc atcgaagagc agggcggcgc gatcgctgcc gagcggttgt
     2341 tcggcgacga tacggctgcg gcgctcgagc gcgaagccga cgggcgacat ccgcatacat
     2401 cggcaccgcc ccgccgctga tgtggccccc ggatggaact tcgcgcagcg attgtcgttc
     2461 acgccctagg caacctgtgg cagcgcggtg aaacaaacgc gccggaggct ggccgaaaat
     2521 cttcggtctc cgctgccgca ccgaatttcg cgcgatggaa tgttggtatc gcggccccgg
     2581 ttcggtccta ggaaaacgac cggaggagca gtctggggga actcaggcca caggcgcctt
     2641 cttgacatgc gcggcatacg ccggccatcg cgatgccgcg ccaaggcctg aggatctata
     2701 atctctttcc gttgctcgcc ggcaccatcg cggagtggcg ggcggagctg ccgcgcatcg
     2761 ccgcgatgcg cttcaattgc gtcttcgtca acccgttcca ttttcccggt ttctccggca
     2821 gcctctacgc ggtaaaggac cattaccgtc tcaacccgtt gctgaagggc gacgcgcgcg
     2881 actcggatga cgcgctcctc gccggcttca ccgccgagtg ccggcgccat gggttgctcg
     2941 ccatgatgga tctcgtggtg aaccatacgg cgcgcgacag cgagctcgtg gcgagccatc
     3001 cccattggtt cgcacgcgag ccgggcggcg gcgtgcgctc gccctcggcc atcgacccgg
     3061 cggacgccac caatatcacg gtatggggcg atctggccga gatcgcgtat gacggcggca
     3121 gcgacgaggc cgagatcatc gcctatttcc aggaggtgat ccggcattac ctcgggctcg
     3181 gctttcgcgg cttccgctgc gatgccgcct acaaggtgcc gccgcgggtg tggcgcgcgc
     3241 tgatcgccgg ggcacgcgcg gtcgcccccg actgcgtctt ctgcgccgag acgctggggg
     3301 cccgcctcga agaggtcgcg cgtctcgacg gcaccggttt cgactatctc ttcaacagcg
     3361 ccaagtggtg ggatttcgag agcccgtggc tcctcgagca atacgaccgc ttccacagga
     3421 tcgcgccctc gatcgcgttt ccggaaagcc acgacacgga gcggctcgcg gcggagctcg
     3481 cgcggagcgg catcgtcgat gccgaggcga tcgcggtgcg gcaccgccag gcctatgcct
     3541 tcgccgcctg cttcgcgacg ggcgtgatga tgcccatggg cttcgagttc gggtggagcc
     3601 ggcgcctgga cgtcgtgaag acgcggcggc tgccggtcga gccgaagcgc ttcgacctct
     3661 ccggcgagat cgccgccatc aacgccatga agcaggcgac gcccgcgctc aacgaagagg
     3721 ggccgcaatc gcggctgacg acgcccgacg accccgtcgt ggccctcgcc cggcggacgg
     3781 cggacggccg ggaatgggcc ttcagcctct tcaataccga cgacacgacg gcgcaggaga
     3841 tcgacgccga cggcctgctc gcggccgcgg acggacacgc gctcctgctc gaggaagtga
     3901 cgccgggcgc cgagccgggc ctcggcctcc acctcgtgct cgagccgctg gcggtgcgcg
     3961 tcctgcgcgg cctgcggccg gcgccggcgc gcgtgccggt caccgagacg ccgccgcgca
     4021 tccaccccga gtggtcggcg aacgcgcgca tcctcatcga ggaggtgcag cccgagctcg
     4081 atggcggccg ctatccggtc aagcgcgtcg tcggcgatac cctcgaggtc caggcggaca
     4141 tcctgcgcga cggccatgac gtgctgcgcg cggcggtgct gtatcggccg gaggatgccg
     4201 gcgaatggcg ccaatcgccg atgcggcttt tcgacaatga ccgctgggtc ggcgccgtgc
     4261 cgctcgaaga gaacaagcgc tattgctata cgatcgaggc ctggaccgac gtcttcgcgt
     4321 cctggcgggc cgatacggcg aaaaagcgcg acgccggcca ggcgaccgaa agcgagctcg
     4381 aggaaggacg ggcgctggtg gtggcggcgc tgccgcgcgc cgaggcgccc gaccggcgcc
     4441 tgctcgagcg gttgctgcgc gactccgatg ccggcgacag cgaaatacgg gccgagatca
     4501 tgctgtcgct tctgctggcc gacgccatgg cgcgcgccgg cgaccgcagc gaccggacgc
     4561 gctaccgccg cgcgctcgag gtcgtcgtcg accggcctcg cgcgcggttt ggcgcgtggt
     4621 acgagatgtt tccgcgcagc caggggcgca tccccgggcg aagcgggacc ttcgaggacg
     4681 cagcagcgcg gctcggcgag atccgggcga tgggcttcga cgtcgtctac ctgccgccga
     4741 tccatccgat cgggcgcctc aaccgcaagg ggcgcgacaa cagcctcacg gtgcgcccgg
     4801 gagatcccgg cagtccctat gccatcggct cgcccgaggg cggccatgat gccgtccatc
     4861 cggagctggg cggcatcgcc gggttccgcg gcttcgtcgc ggcggcgcgc ggcctcggcc
     4921 tcgacgtggc gctcgactat gcgatccaat gcgcgcccga ccatccctgg gtcaaggagc
     4981 acccggagtg gttcgtcttc cgcagggatg gcagcatcaa atacgccgag aacccgccca
     5041 agaagtatca ggacatcgtc aatgtcgatt tctacaaccg ggaccgcgac gggctgtggc
     5101 aggcgctccg cgacattctg ctcttctgga tcgacgaggg cgtgcggatc ttccgcgtcg
     5161 acaacccgca caccaagccg ctgcctttct gggaatggct catcggcgag gtgcaggcgc
     5221 gatatcccga cgtgatcttc ctctccgagg ccttcacgcg gcccaagatg atgcgggcgt
     5281 tggccaaggc cggcttcact cagtcctaca cctatttcac ctggcgcgtg accaaagagg
     5341 agctgatcgc ctacggcacc gagctcgcgc aaggcccggc gaaggaattt ttccggccga
     5401 acttcttcac caacacgccg gacattctgc cgctgcacct gcagcaaggc gggcggccgg
     5461 ccttccgcag ccgcctcgtc ctggcagcga cgatggcgcc cgcctacggc atctacagcg
     5521 gcttcgagct gtgcgagaac ggcgccatcc ccggcagcga ggagtatctc cattcggaga
     5581 aatacgaata caaggtgtgg gactgggatc gtcccggcca tatcaaggac gacgtcgcgc
     5641 gcctcaaccg catccgccgc gagaacccgg cgctccagga gctgaccaat ctgcagttct
     5701 atccggcgga tgacgatcac gtcgtgttct acggcaagat gaccgcggac ggcggcaaca
     5761 ggattttcgt cgccgtcaac ctcgatccct tcgccgtgca tgaagcgacg atcgacctgc
     5821 cgctcgcggc aatgcgcatc gcggaggttg aaactttcac cgcggtcgag ctgttcaccg
     5881 gtgaggagaa gcgctggcgc ggcgcccgcc agcgcctgcg cctcgatccc gacgtcaacc
     5941 cggcggcgat cttccgcgtc gagcggtagg acgctcgccc ttcatgtgga tactcgctct
     6001 ggaccaagca gccaacatcc cctctcctgc atcgcgggag aggatggcga gcgcagagcg
     6061 cgcgcctgcg acgacgcgcc ggggtgaggg ggcatgtcaa gcaaagtcgc cgtgcttgac
     6121 gcgccccctc accctcccag cgcttcgcgc tgggtccctc cctcgcccgc gatgcgggag
     6181 agggtcagga acgcgcgtcg acgcgaatct gcgcgcggtc taggccatgc ctgacaccag
     6241 cgtcgccacg gaagccgcgc cgctcgtcgg cgatccgctg tggtacaagg acgcgatcgt
     6301 ctaccagctc cacgtcaaag cgttcttcga cagcaccgat gacggcatcg gcgatttcca
     6361 ggggctgacg cgcaagctcg actacctggc ggatctcggc gtcagcgcgc tctggctcct
     6421 gcctttctat ccttcgccgc tgcgcgacga cggctacgac atcgccgatt accgcggcgt
     6481 caatccggcc tatggcggct tgagcgactt ccgcgccttc gtgcgcgcgg cgcacacaag
     6541 gggcatgcgc gtcatcaccg agctcgtcat caatcacacc tcggaccagc atccctggtt
     6601 tcagcgggcg cggcgcgcca aggccggatc tttctggcgc aactattatg tctggagcga
     6661 caccgaccag aaattcctcg gcacgcgcat catcttcctc gatgccgaga gctcgaactg
     6721 gggctgggat cccgtcgcca aggcctatta ctggcaccgc ttctatgcgc atcagccgga
     6781 cctgaacttc gacaatcccc gcgtcgtgcg cgaggtggtc aatgtcatgc gctactggct
     6841 cgacatgggc gtcgacggga tgcgcctcga cgccgtgcct tatctcatcg agcgcgaagg
     6901 caccaacaac gagaacctgc ccgagaccca tggcgtgctg aagcggcttc gcgccgagct
     6961 cgaccgtcgc tatggcgacc gcatgctgct ggccgaggcc aatcaatggc ccgaggacgt
     7021 gctgcagtat ttcggcgagg gcgacgaatg ccacatggcg ttccacttcc cgctgatgcc
     7081 gcgcatcttc atggccgtgg cgatcgagga tcgccatccc atcaccgaca tcatgcgcca
     7141 gacgcccgac atcccggcca attgccaatg ggcgatcttc ctgcgcaatc acgacgagct
     7201 gacgctcgag atggtcaccg accgcgagcg cgactatctg tggaagcatt acgccacaga
     7261 caagcgcatg cgcctcaatc tcggtatccg ccggcgcctc gcgccgctga tggagaacga
     7321 ccggcggcgc atggagctgt tgaacgggct gatgttcgcc atgccgggaa cgcccgtcat
     7381 gtattacggc gacgagatcg gcatgggcga caacatcttc ctcggcgacc gcgacggcgt
     7441 gcgcacgccc atgcaatggt cgcccgaccg caatggcggc ttctcgcgcg ccgatccggc
     7501 gcagctttac ctgccgccga tcatggacgc ggtctacggc tttcagtccg tcaatgtcga
     7561 ggcgcagagc cgctcgccgt cgtcgttcct caactggatg aagcgactga tcgccgtgcg
     7621 caaggcgcgg cgcgccttcg gccgcggcga gcagcatttt ctctatccgg cgaaccgcaa
     7681 ggtgctggcg ttcctccgct cggccggcga cgagaccatc ctctgcgtgt tcaatctggc
     7741 gcgcgcgccg caagcggtcg agctcgatct ctcgggtttc aagggacgcg tgccggtcga
     7801 gctgttcggc cgcagcttct tcccgccgat cggcgacctg ccttatctgc tgacgctgcc
     7861 gggtcacggc ttctattggt tcctgctcga cgcgtcgtcg gcggtcgagc cgaactggcg
     7921 cggcgcgtcg ctgccggaat tcgtcactct cgtcatgccg gatggctgga cgggcccgct
     7981 cgaccgccac aaccgccgcc tgttcgagag cgaagtgctg ccggcctatc tgccgaacca
     8041 gcggtggttc gccgggaaga gccagcgcat cacgcaagcg gccgtcgcgg cggtggtcga
     8101 gctgccccgc gccggcggcc agagctggat gctgtcgctc atcgacatcg cgttccgcga
     8161 tggcgagcag cagcgctact tcctgccgct cgccttgacc tgggacgagg aggagagcga
     8221 gctgcgcaag gccaccctgc cttacacgct ggcggtcgcg cgccgcggcc ggcgcgaagg
     8281 cgccgtctat gaagcgaccg ccgatcagga cttcgcgctg gcgctgctgc aggcgatcgg
     8341 cgcgcaggcg gagctcaaag gcccgcgcgg acgcgcccgc ttcgagcgga cgacggcgta
     8401 cgggctggcg ccgccgccgg agaagccgga cgtccgccgc gtcggcggcg agcaatccaa
     8461 cagctcgctc ctcatcgacg agtatggcgt gctcaagctg taccggcgcc tcgccgcggg
     8521 cattcagccg gagatcgaga tgacgcgctt cctcacggag gttgcgcatt tcccgcatac
     8581 cccgccgctg ctcggcgcgg tccacttcag cgacgaggcc ggcggcgagg cggcgctggg
     8641 cgtgctcttc gcctatgtcc gcaaccaggg cagcgcctgg tcgcagtcgc tcaactacct
     8701 gacgcggcat ctcgacgaag ccctgctgat ggcgccgcac gaggcgcggc cgtcggaacc
     8761 gccgacgccc gaggcgctcg cccatccgct ctatctcgag ctcgccgcgc aactgggccg
     8821 gcgcacggcg gagctgcatc gcgcgctgtg tccggacgtg ctggtcgacg acgcgttcgc
     8881 gcccgagccg accaccgccg aggatctcgc gcattggcgc gagcgggcga tcggcgcggc
     8941 gcgcgtcatg ctcgacaacc tggcacgccg ccgcgacgga ctgccggcgc cggcacaggc
     9001 gctggccgac cggctcctgg cgcggcgcga caaggtgccg gagcgcttcg cgtcgctgct
     9061 gccgcccgac atcgcggcgg tgaagacgcg gttccacggc gattatcatc tcgggcaagt
     9121 gctggcggtg caggacgact tctacatcat cgatttcgaa ggcgagccgc tgcgcgcgat
     9181 ggccgagcgg cggcaaaaga gctcgccctt gcgcgacgtg gcgggcatgc tgcgctccta
     9241 cgcttatgcg gcggcgacgg cggtgcggca gatgacggag atacagccgg cggcacagcc
     9301 ggtgctgcag gagcgcgccg aggagtggcg ccgccaggtc tcggatgcgt tcctcacccg
     9361 ctaccgcgcc gagatggcgg gcgtgcgctc gatcccggcg gagtcgggca aagccgcggc
     9421 gctgcttgac ttcttcacgc tcgagaaggc catgtacgag gtcgattatg aactggcgca
     9481 acggcccgcc tgggtggcga tcccgctcgc gggcgtgctg gctctgctcg agggagcttg
     9541 atggcgacac tcagtccggc cgcgcgcgcc atcctcgaag gccgtcacgg cgatcccttc
     9601 tcctttctcg gcctgcatcc ggtgggcggc aatctctgcc tgcgcgtctt cctgccctgg
     9661 ccgcggcggg tctgggcgat cgatgcggcg acgggcgagc tcgcgggaga gctcaaccgc
     9721 atccatgagg acgggctctt ctccggcatg ctgggccacc ggccgccctt tcgctatcgg
     9781 ctgcgcgcag aaggggcgct gggcacgatc gaattcgacg acgtctatgc ctttccgccc
     9841 gtgctcggcg atctcgacat ccatctcctc gccgaaggga cgcatcacga aagctacgcc
     9901 gtcatgggcg cgcattgccg cgagcatgag ggcgtggcgg gcgtctcctt cgccgtctgg
     9961 gcgcccaatg cgcaccgcgt cagcgtcgtc ggcgacttca atgagtggga cgggcgacgc
    10021 tatccgatgc ggctgcgcca tgccgcgggc gtctgggagc tcttcatccc gggcctcgag
    10081 ccaggccatt tctacaaata cgagatcctc ggcgcagagg gcaaggtatt gctcaaggcc
    10141 gatccctatg cgcgccgcgc cgagcatccg ccgggcaccg cctcctgtat cgccgcgccg
    10201 gccaagcatg cctggagcga cgccgaatgg atcgagacgc gctggcggcg caatgaccgc
    10261 catgcgccca tcagcgtcta tgaagcccat ctgggatcct ggcggcgcaa gctcgaggaa
    10321 ggcgggggtt atctgaccta tcgcgagctc gcggagcagc tcgtgcctta tgtgcgcgac
    10381 atgggcttca cgcatctcga gctgatgccc gtcagcgagt atcccttcga cggctcctgg
    10441 ggatatcagc cggtcagtct cttcgcgccg accagccgct tcggcacgcc cgacgatttc
    10501 cgtgccttcg tcgatgcctg ccatgccgcg ggaatcgcgc tgtggctcga ctgggtgccc
    10561 ggccatttcc ccaccgacgc ccatgggctc gcgcgcttcg acggcacggc gctctacgag
    10621 cacgccgatc cgcggcaggg attccagccc gactggaaca ccttcatcta caatttcggc
    10681 cggcgggagg ttgcgaattt cctcttgtcg agcgcgcgct actggatcga cgcgttccac
    10741 gccgacggct tgcgcgtcga tgcggtggcc tcgatgctct atctcgacta cagccgcccg
    10801 gcgggcgagt ggatccccaa cgagcatggc gggcgcgaga acctggccgc cgtcgccttt
    10861 ttcaagcgca tgaacgagct gatcttcggc acgggcacgg gcgctaccac cgcggccgag
    10921 gagtcgacgg cgtggccgat ggtgtcgcgg ccgacctatc tcggcgggct cggcttcggc
    10981 ttcaaatgga acatgggctg gatgcacgac acgctgcgct acatggcgca ggatccgatc
    11041 taccgccgct accaccacaa cgatctcact ttcggcctgc tctacgcctt ccatgagaac
    11101 ttcatcctgc cgctctccca tgacgaggtc gtgcatggca agggcgcgct catcgccaag
    11161 atgcccggcg accgctggca gcgcttcgcc aatctgcgcg cctattacgg cttcctgtgg
    11221 acgcatccgg gcaagaagct gctgttcatg ggcggcgagt tcgcgcagga gcgcgaatgg
    11281 aatcacgacc gcggcctcga ctggcaattg ctcgccgatc ccatgcaccg cggcgtgcag
    11341 atggtcgtgc gcgacctgaa ccatctctat cgcgagctgc cggcgctcca tgcgctcgat
    11401 gccgagcccc gaggcttcca gtggatcgac ggcagcgatg ccgaccagag cacgatctct
    11461 tatatccggc ggagcgagga cccgcagacg ctggccgtcg tcctgtgcaa tttcacgccg
    11521 gtgccgcgcc acggctatcg catcggcgtg ccgcagagcg gcctttatcg cgagcgcttc
    11581 aacagcgacg ccatcgagta tggcggcagc ggcctcggca atgccggcgc cgtccgaacc
    11641 gaggcccatc cgagccacgg ccacgcccat tccctggtcc tgacgctccc cccgctcgcc
    11701 acgctcatct tcgcccatga gccggagagc ggctgagacg ggagcggacg gctcttttcc
    11761 tcgggcgcgg tgttctctct tggcgcagcg gggagacgag gatgaaacgc catcatgagc
    11821 tgcccttcgg cgccgagctg gtcgccgggg gcgtgcg
//
LOCUS       DASYNS010000548         6181 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2635380, whole genome shotgun sequence.
ACCESSION   DASYNS010000548 DASYNS010000000
VERSION     DASYNS010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2635380"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..602)
                     /locus_tag="VGP50_16830"
     CDS             complement(171..602)
                     /locus_tag="VGP50_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029100.1"
                     /translation="MQQSATPSVEEVAALVASGLTDAQLGRVFGRGDGWAYKFRRRHG
                     IASARPRPAPRPDRAEIDAANRAYGVQPPSPLSEARLAAFFRGQRRRYEDVSAEELGR
                     ERRGEARGRSSRSPNVTAAVLGDPAPGRLARGEALRPRRCS"
     gene            1081..1761
                     /locus_tag="VGP50_16835"
     CDS             1081..1761
                     /locus_tag="VGP50_16835"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV8029101.1"
                     /translation="MEEFKVARAVERRMKALALNPKSLSKKAGLNETFVRDLLEGRSH
                     NPRTDSLKKLAKALGCTVDDLAEDRPNSATHLHSNRARAEEDHLSVARIDELDVRAAA
                     GPGQNIDSEAKIGEWQLPRELVKIATHSPVERIKILTIVGDSMEPTYKPTEKVMVDTG
                     DVRPSPPGIFVVWDGLGFVVKRIEFLPHSDPPRVKITSDNPKYMAYERVLDEAYVQGR
                     VLGKWLWV"
     gene            1859..2278
                     /locus_tag="VGP50_16840"
     CDS             1859..2278
                     /locus_tag="VGP50_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029102.1"
                     /translation="MPEYSATAPSHPPDAVERDAIGAGIVHLGRAAAHLDRLRHLLDL
                     AAACSDLCVVPGAGELLLRLETALALGRGMASAAIERSAARARATGIQARRCLPSPTY
                     DHLFKLIADHGRTLCVMVPGHQGYDPDYWEEVPPVTR"
     gene            complement(2245..3438)
                     /locus_tag="VGP50_16845"
     CDS             complement(2245..3438)
                     /locus_tag="VGP50_16845"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV8029103.1"
                     /translation="MTKERLQTLIIGGGIGGLTAALALARQGRSVHVLEKAGDFAEIG
                     AGLQLAPNALHVLDRLGVLQELAGHAVYPSRLVWMDAILGSRITAIDLGDAFRHRYGQ
                     PYIVMHRGDLLTVLLEACRREALITLEARREVVAVEDLGDAARIRCADGTAYEAELLI
                     GADGLWSTVRRSIADDEPVCAEYVAYRGTIPMAQMSSHAGLDNVVMWVGPEMHFVQYP
                     VRRGELYNQVAVFRSRRYRADSDDWGSPDELDAHYAQTCADVRDGVKLMGRDKRWPMY
                     DRVPIARWTRNRITLLGDAAHPMLQYLAQGACQAIEDSLALAQSVAAAPDDLPRALAA
                     YEEARRLRTARVQITARAMGAFFHLDGVAATLRNAMMAGRASDDYAMLDWLHGHRVTG
                     GTSSQ"
     gene            3509..4060
                     /locus_tag="VGP50_16850"
     CDS             3509..4060
                     /locus_tag="VGP50_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV8029104.1"
                     /translation="MALASSSTALGWKAPDFALEATDGRRYRLGDLCGPKGTLVMFIC
                     NHCPYVQAVLDDVIAEVRELQPLGIGAAAIMPNDTAAYPADSFDNMRRLALAKSFPFP
                     YLIDATQQVARDYGAVCTPDFFGFNAALELQYHGRLYEVRSLKRVAGARRELFEAMAA
                     IARDGAAPAEQAPSIGCSIKWRG"
     gene            complement(4057..5322)
                     /locus_tag="VGP50_16855"
     CDS             complement(4057..5322)
                     /locus_tag="VGP50_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002730938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV8029105.1"
                     /translation="MSTLRLGVIGLGYVGLPLAVALAKHFPVFGYDISARRIAELRKG
                     SDRTDEVAPDALRASSLRVSGEARDLAGSDVFIVTVPTPVDDAHRPDLTAVLASCETV
                     GKQLAGGAVVVFESTVYPGVTEAICGPALEAASGLACGRDFFLGYSPERINPGDREHT
                     VERITKVIAGQTPQVVEILRRVYGAITGDNVFVAKNIKTAEAAKVIENAQRDINIAFI
                     NEVTMIFQKLGISAHDVLDAAATKWNFLRFTPGLVGGHCIGVDPFYLAHRALEVGHKP
                     EIILAGRRINDSMGAFIAERVDSQVPRRSRILVLGLTFKENIPDLRNSKVVDIVNALR
                     GCGHEVVVHDPLADAEEALQHYGIALAPTLEGIGKFHAVIGAVAHATYRALDTRAFAA
                     LLEREGLVADIKGMWRDLRLPEGARRWEL"
     gene            5492..5962
                     /locus_tag="VGP50_16860"
     CDS             5492..5962
                     /locus_tag="VGP50_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029106.1"
                     /translation="MIARLVGAPIVTQSSELSIAQAEVWRQRAARYRTLSALLGKTEN
                     DGRLLALAIELESRASDRARMTAERQDLKSRTQVLLAEVDAFLTHAQATLAQARRLVP
                     LSRYRAEDVREESRLCREEAATAEQVAGKRAVASRAFALAQLAERMDRDAAEAP"
BASE COUNT         1054 a         2103 c         2057 g          967 t
ORIGIN      
        1 ggcgaccatc ttcgcgatcg gatccggccg caatttcgct tccgtctcgc gcgtcggctt
       61 gacatagccg agctcgcggc cgtcgaggat gaaggaatgg cgcgtgcgct cgaaatggtc
      121 ctgctcggcc gcgcgcagcc gtgcgaggcg cgcccgatca tcgcgcgcgc tcatgagcac
      181 cgccgcgggc gcagcgcctc gccgcgggcg agacggcccg gcgccggatc gccgagcacc
      241 gccgcggtga cattcggcga gcgggagctg cggccccgtg cctcgcccct tcgctcgcgg
      301 ccaagctcct cggccgacac gtcctcgtag cgccggcgct gcccgcggaa gaaggcggca
      361 agccgggctt cgctgagcgg cgacggcggc tgcacgccat aagcgcggtt ggctgcgtcg
      421 atctccgcgc ggtcgggacg gggcgccgga cgcggccgcg ccgaggcgat tccgtgccgc
      481 cgccggaact tgtaggccca gccatcgccg cgcccgaaaa cgcgcccgag ctgcgcgtcg
      541 gtgaggccgc tcgccaccag cgcggcgacc tcctcgaccg agggcgtggc gctctgctgc
      601 aaagaagcgg ccggcgcccg aaaccccggg gcgaagcccg ggggttgctc cggtggggcg
      661 ccggccgcag gtcccgtcgc cgccggggga gacggcgacg gatcgcggac aagggagggg
      721 ttgccgcgaa tgacggaagc cgcgtgtcgc tcggacatca ccgtggtgac tgcggcttgg
      781 gacggcgccg cttctgcgct cacgaccggg cacggcagcg ccgcagccag cgtgccgctt
      841 tcgtcaggtg gcggtcggcg cggcggagat ggcgcacgca cagcattgcc gcgaaccagg
      901 tcggccatcg catgatcgct cccgtaccat cgatcggtcg gatcgcttgc cggccacgac
      961 ccgcgaggcg cgccggcaag cctgttccga tgatgggaaa ttgtagtggg aaatatccct
     1021 cggagtcaat gggaaacttc cccgctcccg ccccgcgcgc atctgtggga tgactgcgcg
     1081 atggaggagt tcaaagtcgc gcgcgccgtc gagcgccgga tgaaggcgct ggcgctcaac
     1141 cccaagagcc tgtcgaagaa ggccggcctc aacgagacct tcgtgcgcga cctcctcgaa
     1201 ggccgctcgc acaacccgcg caccgactcg ctcaagaaac tggcgaaggc tctaggttgc
     1261 accgtggacg atctggcgga ggatcgccca aattccgcca cacacttgca ctcaaataga
     1321 gcacgcgccg aagaggatca tctctcggtg gcacgcattg acgagctcga cgtgcgcgca
     1381 gcggcaggtc cgggccagaa tattgatagt gaagccaaaa tcggcgagtg gcagctcccg
     1441 cgggagctcg tgaagattgc cactcactcg ccggtcgagc gcatcaagat tctcaccatc
     1501 gtcggcgaca gcatggagcc gacctacaag cctaccgaaa aagtgatggt cgacaccggc
     1561 gacgtccgcc cgagcccgcc cggcatcttc gtggtctggg acgggttggg cttcgtcgtg
     1621 aagcgcatcg agttcctgcc ccattccgat ccgccgcggg tcaagatcac gtccgacaac
     1681 ccgaaataca tggcttatga gcgggtgctc gacgaggcct acgttcaggg ccgcgtcctc
     1741 ggcaaatggc tctgggtctg aaaactgccc ggtgcgggca agatatggtc ccaccgaccc
     1801 tctttgacga tgggattatt cccatcaaca ttggctcgcc acgcccgctg gagagccgat
     1861 gccggaatac agcgccaccg cgccctctca tccgcctgac gccgtcgaac gcgacgccat
     1921 cggcgcgggg atcgtccacc tcggccgcgc cgcggcccat ctcgatcgcc tccgccatct
     1981 gctcgatctt gccgccgcct gctccgacct ctgcgtcgtg cccggcgcgg gcgagcttct
     2041 cttgcggctg gagaccgcgc tcgcgctcgg ccgcggcatg gcttccgccg ccatcgagcg
     2101 cagcgccgcg cgagcgcgcg ccacgggcat ccaggcacgg cgctgcctgc cgagcccgac
     2161 ctatgatcat ctcttcaagc tgattgccga ccatggccgc acgctctgcg tcatggtccc
     2221 gggacaccag ggctacgatc ccgactattg ggaagaggtg ccacccgtga cacgatgacc
     2281 gtggagccag tcgagcatgg cgtaatcgtc gctcgcgcgc ccggccatca ttgcgttgcg
     2341 cagggtcgcg gccacgccgt cgagatggaa gaaggcgccc atggcgcgcg ccgtgatctg
     2401 gacgcgcgcc gttcggaggc ggcgcgcctc ttcgtaagcg gcgagcgcgc gcggcagatc
     2461 gtcgggtgcg gccgcgacgc tctgcgcgag cgcgagtgaa tcctcgatcg cctggcacgc
     2521 gccctgggcg aggtattgca gcatcggatg cgcggcatcg ccgagcagcg tgatgcggtt
     2581 gcgcgtccat cgcgcgatgg gcacgcggtc atacatcggc cagcgcttgt cgcgacccat
     2641 gagcttcacg ccgtcacgca catcagcgca ggtctgcgca taatgcgcat cgagctcgtc
     2701 gggtgagccc cagtcatcgg agtcggcccg gtagcgccgg ctgcggaaca ccgcaacctg
     2761 gttgtagagc tcgccgcggc gcaccggata ctgcacgaaa tgcatctcgg gcccgaccca
     2821 catgacgacg ttgtcgaggc ccgcatggct cgacatctgc gccatcggga tggtgccgcg
     2881 atacgccacg tattcggcgc agaccggctc gtcatcggcg atcgagcggc gcacggtcga
     2941 ccacaagccg tcggcgccga tcagcagctc cgcctcatac gccgtgccgt cggcgcagcg
     3001 gatccgcgcg gcgtcaccca gatcctcgac cgcgacgacc tcgcgccgcg cctcgagggt
     3061 gatcagcgcc tccctccggc atgcctcgag caacaccgtc agcagatccc cgcgatgcat
     3121 gacgatgtag ggctggccat agcggtgacg gaaggcgtcg ccgagatcga tagcggtgat
     3181 gcggctgccg aggatcgcat ccatccacac gagccggctc ggatagacgg cgtgcccggc
     3241 gagctcttga aggacgccga gacggtcgag cacatgcagc gcgttcggcg cgagctgcag
     3301 cccggcgccg atctcggcga aatcgccggc cttctcgagg acatggacgg agcgcccctg
     3361 gcgggccagc gccagtgcgg cggtcaggcc gccgatcccg ccgccgatga tcagagtctg
     3421 aagcctctct ttcgtcaaaa ggtcatccct cctgcggcgc acatgactga gctaagctca
     3481 gggccagccc aaagcacgga ggaacggcat ggcgctcgca agctcttcga ccgctctcgg
     3541 atggaaagcc cccgacttcg cgctggaggc gaccgatggt cggcgctatc gcctcggcga
     3601 tctctgcggc ccgaagggga cgctggtcat gttcatttgc aaccactgcc cctatgtgca
     3661 agcggtgctc gacgacgtca tcgccgaagt gcgggagttg cagccgctcg gcatcggcgc
     3721 tgccgcgatc atgcccaacg acaccgccgc ctatcccgca gactctttcg acaacatgcg
     3781 ccgcctggcg ctggccaaga gcttcccctt tccttatctc atcgacgcga cgcagcaggt
     3841 ggcgcgcgac tatggcgccg tctgcacgcc cgatttcttc ggcttcaacg cggcgctcga
     3901 gctgcaatat catggacgcc tctacgaggt ccgcagcttg aagcgcgtgg cgggcgcgcg
     3961 ccgcgagctt ttcgaggcca tggcggcgat cgcgcgcgac ggcgccgcgc cggcggagca
     4021 agccccgagc atcggctgct cgatcaaatg gcgcggctag agttcccagc gccgcgcgcc
     4081 ttccggcagg cgcagatcgc gccacatgcc cttaatgtcc gcgaccaatc cctcgcgctc
     4141 gaggagcgcc gcgaaggccc gcgtgtcgag cgcgcgataa gttgcatgag caacggcgcc
     4201 gatgacggcg tggaacttgc cgatgccctc gagcgtcggc gccagggcga tgccgtagtg
     4261 ttgcaacgcc tcttcggcat cggccagcgg atcatgaacg acgacctcgt ggccgcaccc
     4321 gcgcaaggcg ttgacgatat cgaccacctt ggaattgcgc aggtcgggta tgttctcctt
     4381 gaaggtcagt ccgaggacga gaatgcgcga gcgtcgcggc acctggctgt cgacgcgttc
     4441 ggcgatgaag gcgcccatgg aatcattgat ccgccgtccc gccaggatga tctccggctt
     4501 gtgcccgact tcgagcgcgc ggtgggccag gtagaacggg tcgacgccga tgcaatgccc
     4561 gcccaccaga cccggcgtga agcgcaggaa attccacttc gtcgccgcgg cgtcgagaac
     4621 atcgtgtgcg gatatgccga gcttctggaa gatcatcgtc acttcgttga tgaaggcgat
     4681 attgatgtcg cgctgcgcat tctcgatgac cttggcggct tcggcggtct tgatgttctt
     4741 ggcgacgaag acgttgtcgc cggtgatcgc gccataaacc cggcgcagga tctcgaccac
     4801 ctgaggcgtc tgtcccgcga tcaccttggt gattctctcg accgtatgct cccggtcgcc
     4861 cggattgatg cgctcgggcg agtagccgag aaagaaatcg cggccgcagg cgagccccga
     4921 agccgcttcg agcgccggcc cgcagatggc ctcggtgaca cccgggtaga cggtgctctc
     4981 gaaaacgaca acggcgccgc cggcgagttg cttgccgaca gtctcgcagg acgccagcac
     5041 cgccgtcaga tcaggccggt gtgcgtcatc gaccggcgtc ggcaccgtga cgatgaacac
     5101 gtcgctgccg gccagatccc gggcctctcc gctgacgcgc aggctgctcg cccgcaacgc
     5161 atccggcgcg acctcatccg tccgatcgga gcccttgcgc agctcggcga tacggcgagc
     5221 gctgatgtca tagccgaaga ccgggaaatg cttggcaagc gcgaccgcga gaggcaggcc
     5281 cacataaccc aacccgatca caccgaggcg aagcgtcgac atggggcagc ccctctcgtt
     5341 gtcagcgatt tagcccgcaa gagccgcggc cccgccaccg ggagcgtcgc cgtataagaa
     5401 acggcagcca cagcgtcaag caagcggagg ctggggggct ggccagaccg gagggtaccg
     5461 ttatcgtttg cgctccctca gtgaacgggt catgatcgct cggctggtcg gagcaccgat
     5521 cgtgacacag tcgtccgagc tatctatcgc gcaagccgag gtttggcggc aacgcgcggc
     5581 acgctatcgc acgctttcag cgctcctggg caagaccgag aatgacgggc gtctgctggc
     5641 actggccatc gagcttgaga gccgggcgag cgatcgcgca agaatgaccg cggagcgaca
     5701 ggatctcaaa tccaggacgc aggtcttgct cgccgaggtc gacgcgtttc tgacgcacgc
     5761 gcaggcaacc ctggcgcaag cgcgccgcct cgttccgctt tcgagatata gggccgagga
     5821 cgtgcgggag gagtctcgcc tgtgcagaga agaagcggcg accgctgagc aagtggccgg
     5881 gaagcgggca gtcgcgtcgc gtgccttcgc gctggcgcaa ctggccgaga gaatggaccg
     5941 ggacgccgcg gaagcgcctt gacgcggcgc ttccctccgt cacgcgctcg cgaacggagt
     6001 tgagtggtct ttgctacctt ggcgcccgac caccaccgga cgacaacacg gccgcctttt
     6061 cctgctcgag cgccgcaagg aggaaggtca tgcgcccttg ttcgacgggt gcgcgtgatt
     6121 taaaggcagc aatcgactct tcgaggcgcc gaagctgtcg gtcgtcgtgg gcaatcagta
     6181 c
//
LOCUS       DASYNS010000549         4576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3882989, whole genome shotgun sequence.
ACCESSION   DASYNS010000549 DASYNS010000000
VERSION     DASYNS010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3882989"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VGP50_16865"
     CDS             <1..379
                     /locus_tag="VGP50_16865"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEV8029107.1"
                     /translation="PVNTLQLMRGAVAAQFEGVFEPYCRAAWHHMWEDPKKLDDPQVA
                     REAFLSSNIDYDRLSARAQESHIKNRLIALTQNAVDRGAFGSPTFFVGADMFFGKDQL
                     RDVEEAIVAAGAAAGSEWRPSAR"
     gene            415..1158
                     /locus_tag="VGP50_16870"
     CDS             415..1158
                     /locus_tag="VGP50_16870"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8029108.1"
                     /translation="MTARNQTVAVIGAGDHIGAEIAKKFAAEGFSLFVGRRNGDKLAP
                     LVKEIEAAGGSAFARTLDARKEEEVTSFLADADKHAPLEVCVFNIGANVNFPLLETTE
                     RVFRKVWEMACYSGFLAGREAARLMLPRGRGSIFFTGATASLRGGIGYAAFASAKFGL
                     RAVAQAAARELWPKNIHVAHLIIDAGVNTAWVRDRIKEREGQAALDNLDPERLMPPAA
                     VADAYWQLYGQPRSAWVFEQEIRPFGEKW"
     gene            1185..2189
                     /locus_tag="VGP50_16875"
     CDS             1185..2189
                     /locus_tag="VGP50_16875"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEV8029109.1"
                     /translation="MKTAITEMFGIRHPIIQGGMYYVGFAELASAVSNAGGLGIITGL
                     TQKTPELLAGEIARCRAMTDKPFGVNLTFLPSFTAPPYPEYIAAIVEGGVKIVETAGR
                     SPEQYMPALKAAGIKVIHKCTSVRHSLKAERIGCDAVSVDGFECGGHPGEDDIPNMIL
                     LPRAADALKIPFVASGGMADARSLVAALALGAAGMNMGTRFMATKEAPVHQNVKEAIV
                     AASELDTRLIMRALRNTERVLKNAAVEKLVGIEREKGVSLTVADIHDQVGGVYPRIML
                     NGEMDAGAWSCGMVVGLIHDIPTVAELIDRIMREAEQLIRHRLAGLLGRDESPAMARA
                     "
     gene            2230..2934
                     /locus_tag="VGP50_16880"
     CDS             2230..2934
                     /locus_tag="VGP50_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8029110.1"
                     /translation="MMAQPLSIITGVGPGTGSALARRFAAGGHRVAMLARNAERLAQL
                     EREIPGSKAFVCDVSDAAQVEAAAQRIEGELGAPEVLVHNAVGGAFGSFLEIDPAVLD
                     RNFQVNTMGLLYLARRFAPAMVRAGRGTIIVTGNTSALRGKAGFAGFAPTKAAQRILA
                     EAMARDLGPKGVHVAYIIIDAVIDVPRMRERFREKSDEFFAKPAAIAEEAWHLTQQDR
                     SAWSFTVELRPYGETW"
     gene            2931..3749
                     /locus_tag="VGP50_16885"
     CDS             2931..3749
                     /locus_tag="VGP50_16885"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEV8029111.1"
                     /translation="MSPAMPALRIFSYLPNPRLWKATIAGRLCGVAIELRGAAPGELS
                     QWLWDFDARPLAEVPPEMLATTERAGRIGFSKSKLHKTAAFLEAHPFGTVPAAFSPDG
                     RTGIFESNSIMRTVARLGQERFPLYGRDAYEASRIDSFLDASLVFARDSQIYLLALRG
                     GPVTPELYARMRESFETYLSGIEQALTPERKFLVGDDLTLADICFAAELCLFHNERAR
                     RSALEAAGLPLLLGDAFAQTYPRSSDHFQRLLAHPAFVPDVAGYLEKIERSSAR"
     gene            3852..>4576
                     /locus_tag="VGP50_16890"
     CDS             3852..>4576
                     /locus_tag="VGP50_16890"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEV8029112.1"
                     /translation="MNVSIPRRSVVTAALLGVALLSRASPDAIAADASDRDVQVNLGV
                     LDSLGPAPDASATGTAPIHLHPPRPKINATHAAADAPHAPAPAVATAAPPRNGAPAKA
                     AEDAASSAPPSAGGNPPPAATAAAVATPAPPPAPAPVTAMNSPPAVKSPATNVAPAAA
                     HPAAPKAPPPPTRLLFATGAVDLPDGDKPKLDTVVEWLDTNQQARIEVVAYAAGSAEQ
                     ANDARRTSLTRALAVRKYLADHGV"
BASE COUNT          740 a         1618 c         1500 g          718 t
ORIGIN      
        1 tcccgtgaac accttgcaat tgatgcgggg cgcggtcgcg gcgcagttcg agggcgtgtt
       61 cgagccatac tgtcgcgccg cctggcacca catgtgggaa gatcccaaga agctcgacga
      121 tccccaggtc gcgcgggagg cgttcctgtc gtcgaacatc gactacgacc gcttgtcggc
      181 gcgcgcccag gaatcccaca tcaagaaccg cctcatcgcg ctgacgcaga acgcggtcga
      241 tcgcggcgct ttcggttcgc ccaccttttt cgtcggcgcg gatatgttct tcggcaagga
      301 ccagctgcgt gacgtcgagg aggccatcgt cgcggctggc gcggcggcgg gcagcgaatg
      361 gagaccgtcg gcgcgctgag gagtgttcgt tcccctctgt ccaggagacc ggtcgtgacg
      421 gcaaggaacc agacggtcgc cgtgatcggc gccggcgacc atatcggcgc cgagatcgcc
      481 aagaaatttg ccgcggaggg cttcagcctg ttcgtcggcc ggcgcaacgg cgacaagctc
      541 gccccgctcg tcaaggagat cgaggcggcc ggcggcagcg ccttcgcgcg cacgctcgac
      601 gcgcggaagg aagaagaggt cacgtccttt ctcgccgacg ccgacaagca cgctcccctc
      661 gaggtctgcg tcttcaacat cggcgccaac gtcaatttcc cgctgctcga gaccaccgag
      721 cgcgtgttcc ggaaagtgtg ggagatggcc tgctactccg gcttcctggc cgggcgcgag
      781 gcggcgcggc tcatgctgcc gcggggccgc ggcagcatct tcttcacggg cgcgacggcg
      841 agcctccgtg gcggcatcgg ctacgccgcc ttcgccagcg ccaaattcgg gctgcgcgcg
      901 gtggcgcagg ccgcggcgcg cgaattgtgg ccgaaaaaca ttcatgtggc ccatctgatc
      961 atcgatgccg gcgtgaacac cgcctgggtc agggaccgca tcaaagagcg cgaaggccag
     1021 gcagcgctcg acaacctcga tccggagcgc ctgatgccgc ccgccgccgt cgccgacgcc
     1081 tactggcagc tctacgggca gccgcgcagc gcctgggtgt tcgagcagga aattcgcccc
     1141 ttcggcgaaa aatggtgacg tccgctcgag gcgaggaggt tcgcgtgaag acagccatta
     1201 cggagatgtt cggcatccgc catcccatca ttcagggcgg catgtattat gtcgggttcg
     1261 ccgagctcgc ctcggccgtc tccaatgccg gcggcctcgg catcatcacc ggattgacgc
     1321 agaagacgcc cgagctcctg gccggggaaa tcgcgcgctg tcgcgccatg accgacaagc
     1381 ccttcggcgt gaacctcacc tttctcccga gcttcaccgc gccgccctat ccggaataca
     1441 tcgcggctat cgtcgaaggc ggcgtcaaga tcgtcgagac agccgggcgc agccccgagc
     1501 agtacatgcc ggcgctcaag gcggcgggaa tcaaggtcat tcacaaatgc acctcggttc
     1561 ggcactcgct caaggccgag cggatcggct gcgacgccgt cagcgtcgac ggcttcgaat
     1621 gcggcggcca tcccggcgag gacgatatcc ccaacatgat cctgcttccc cgcgccgcgg
     1681 acgcgttgaa gatcccgttc gtcgcctcgg gcggcatggc ggatgcacgc agcctggtgg
     1741 cggcgctcgc gctgggcgcg gccggcatga acatgggcac gcggttcatg gcgacgaagg
     1801 aggcgccggt ccatcagaac gtcaaggaag cgatcgtcgc ggcaagcgaa ctcgatacgc
     1861 ggctcatcat gcgcgcgctc cgcaacaccg agcgggtcct gaagaacgcc gccgtcgaga
     1921 agctggtcgg gatcgagcgc gagaagggcg tgagcctgac cgtcgccgac atccatgatc
     1981 aggtcggcgg cgtctatccc cggatcatgc tcaacggcga gatggatgcg ggtgcgtgga
     2041 gttgcggcat ggtcgtcggc ctgatccacg acataccgac cgtcgccgag ctgatcgacc
     2101 ggatcatgcg ggaggcggag cagttgatcc ggcatcgcct tgccggcctc ctcggccgcg
     2161 acgaatcgcc ggcgatggcg cgcgcgtgac ggggaacgtc gagaagacag cgcgcccgga
     2221 gaggagggca tgatggcaca accgctctcg atcattacgg gcgtcggtcc cggcacgggc
     2281 tcggcgctgg cacgccgctt cgcggcgggc ggccaccggg tcgccatgtt ggcgcgaaac
     2341 gccgagcgcc tcgcccagct cgagcgggaa attcccggaa gcaaggcctt tgtctgcgac
     2401 gtctccgatg ccgcgcaggt cgaggcggcg gcccagcgca tcgaaggcga gctcggcgcg
     2461 cccgaggtcc tggtgcacaa cgccgtgggc ggggccttcg gctcgttcct cgagatcgac
     2521 ccggcggtgc tcgaccgcaa tttccaggtc aacacgatgg ggctgctcta tctcgcgcgc
     2581 cgcttcgcgc ccgccatggt tcgcgccggc cgcggcacca tcatcgtcac cgggaacaca
     2641 tccgcgctgc gcggcaaggc cggtttcgcg ggcttcgcgc cgacgaaggc cgcgcagcgc
     2701 atcctcgccg aggcgatggc gcgcgacctc ggacccaagg gcgtgcatgt cgcctatatc
     2761 atcatcgatg cggtgatcga cgtgccgcgc atgcgcgaac gcttcaggga aaagtccgac
     2821 gaattcttcg ccaagccggc ggcgatcgcc gaggaggctt ggcacctgac ccagcaggat
     2881 cggagcgcct ggtccttcac ggtcgagctg cgcccctatg gcgaaacctg gtgagcccgg
     2941 cgatgcccgc cttgcgcatc ttctcctact tgcccaatcc gcgcctctgg aaggcgacga
     3001 tcgccgggcg tctctgcggc gtcgcgatcg agctgcgcgg cgccgcgccc ggcgagctct
     3061 cgcaatggct ctgggatttc gacgcccgcc cgctcgcgga ggtaccgccc gaaatgctgg
     3121 ccactaccga gcgcgcgggg cgcatcggct tcagcaagag caagctccac aagaccgccg
     3181 cctttctcga ggcgcatccc ttcggcacgg ttcccgccgc tttcagtccg gatggccgca
     3241 ccgggatctt cgaatcgaac agcatcatgc ggaccgtggc ccggctcggc caggagcgct
     3301 ttcccctcta cggccgcgat gcctatgagg cgtcccggat cgacagtttt ctcgatgcca
     3361 gcctggtgtt cgcgcgcgac agccagatct accttctggc cttgcgcggc ggcccggtca
     3421 cgccggagct ctacgcgcgc atgcgcgaga gcttcgagac ctatctgagc ggcatcgagc
     3481 aggcgctgac gcccgagcgc aagtttctcg tcggcgacga tctgaccctc gcggacatct
     3541 gcttcgctgc cgagctctgt cttttccaca acgagcgcgc ccgcagatcc gcgctcgagg
     3601 ccgccggcct tccccttctc ttgggcgacg cgttcgccca gacgtatccg cgatcgtcgg
     3661 atcattttca gcgtcttctc gcccatcccg ccttcgtgcc ggacgtcgcg ggctacctcg
     3721 agaagatcga gcggagcagt gctcgctgag gctgagtgtt tccagcccgg aagcgacgcc
     3781 tgaaaccggg acaatccgcc gcaagctggc tgtggcggtg gcgacacagt ttgcgctatt
     3841 ttgtcaggga catgaacgtc tcgatccctc gccgctccgt cgtcaccgcc gccctgctcg
     3901 gcgtcgcgct gctctcgcgc gcgagtcccg acgcgatcgc cgccgatgcg tcggaccgcg
     3961 atgtccaggt gaatctcggc gttctcgatt cactcggtcc cgcacccgac gcatccgcga
     4021 ccggcacggc gccaattcat ctccacccgc cgcggcccaa gatcaacgcg acgcatgccg
     4081 ccgcagacgc gccgcacgcg ccggctcctg ccgtcgcaac ggccgcgccg ccacgcaacg
     4141 gcgcacctgc gaaggctgcg gaagatgccg cttcttccgc accgcccagc gccggcggca
     4201 accctcctcc cgcggcaacg gcagccgccg tggcaacgcc agcgccgcct ccggcaccgg
     4261 cgcccgtcac cgcgatgaat tcgccgccgg ccgtcaagtc gccagcgacg aacgtcgcgc
     4321 cggcggccgc tcaccccgct gcgcccaagg cgccgccgcc gccgacgcgc ctgctcttcg
     4381 ccaccggcgc cgtcgatctc cccgatggcg acaagcccaa gctcgatacc gtggtcgaat
     4441 ggctcgacac caatcagcag gcgcggatcg aggtcgtggc ctatgcggcg ggcagcgccg
     4501 agcaagcgaa tgacgcacgg cggacgtcgc tcacgcgcgc gctcgcggtg cgtaaatacc
     4561 tggccgacca cggcgt
//
LOCUS       DASYNS010000550         7253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1857099, whole genome shotgun sequence.
ACCESSION   DASYNS010000550 DASYNS010000000
VERSION     DASYNS010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857099"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /gene="modC"
                     /locus_tag="VGP50_16895"
     CDS             complement(<1..899)
                     /gene="modC"
                     /locus_tag="VGP50_16895"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HEV8029113.1"
                     /translation="MIEIDVEKRLGAFTLAARFNTPAQGITALFGRSGSGKTTLVNML
                     AGLVRPDRGRIAIGGDVLFSSEDGIDLPPERRRLGYVFQEGRLFPHYSVRGNLTYGRR
                     HGTARDAPGLDAIVALLGLEGLLDRRIGDLSGGEKQRVAIGRALLAEPRLLLMDEPLA
                     ALDTPRKAEILPFIERLRSELGIPVIYVSHAMEEILRLADMLVLMSEGRVAAIGTVEE
                     LTSRIDLRPLTGRYEAGSVIRATVAGQDVSYGLTELAFPGGRLRVAHVALPLGTAVRA
                     RIRARDVALALERPTGISIRNILP"
     gene            complement(896..1594)
                     /gene="modB"
                     /locus_tag="VGP50_16900"
     CDS             complement(896..1594)
                     /gene="modB"
                     /locus_tag="VGP50_16900"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HEV8029114.1"
                     /translation="MLTLSPLESEALLLSLRVGVVSVAVSLPFGLAIAWLLARHDFPG
                     KILLNGLVHLPLVLPPVVVGYSLLVLFGRHGPVGAWLYESLGITFAFNWKGAALAAGV
                     MAFPLMVRAMRLSLELVDQRLEAAARTLGARPLRVLATVTLPLMAPGILTGAILAFAR
                     SISEFGATITFVSNIPGETRTLPLALYALTQTPGGEASAWRLAILSVLLAMAALVASE
                     LMARRLDRRIRGLA"
     gene            complement(1803..2984)
                     /gene="metC"
                     /locus_tag="VGP50_16905"
     CDS             complement(1803..2984)
                     /gene="metC"
                     /locus_tag="VGP50_16905"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020591858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV8029115.1"
                     /translation="MRSKSKKPDTLLTHAGRDPATCRGAVNPPVYHASTILHPSVAAL
                     EAAQRNRYDEIAYGRYGTPTTRAFEDAVAAVEGGHRAVAFCSGAAACFATLLAYLKAG
                     DHVLVPDNVYGPVRGFCNGFLERFGVHSTFYDPLIGEGIAGLVRDNTRVIYLESPGSL
                     TFEVQDVPAIVAVAKARGIVTMLDNTWAAPLFLKPLALGVDVSIIAATKYIVGHSDAM
                     MGIAVCTETSFMPVRETATLLGNHAAPDDCYLALRGLRTAALRLRQHELQGLALARWL
                     AARPEVERVLHPALPGCPGHDFWKRDFSGSSGLFSIVLRADIAKPAIDAMLDGMELFG
                     LGFSWGGFESLMVQVRPDRLRSAKPWQSGPVLRIHAGLEDQGDLIADLEHGFARLNAQ
                     R"
     gene            3153..4181
                     /locus_tag="VGP50_16910"
     CDS             3153..4181
                     /locus_tag="VGP50_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV8029116.1"
                     /translation="MDSVRMSTALGVAVAVVAAAGAAVAGPTLDSVKSRGVVTCAVNT
                     GLVGFGMPDQQGDYKGLDVDTCRAIAAAVLGDAKKVKFIPATTQQRFTVLQSGEADVL
                     TRNTTETLLRDTEQGFNFAPVTYYDGQGFMVPKKLGVKSAKELNGATVCVQPGTTTEL
                     NLADYFRANKMSFKPLTIEKLAEVEDAFFSGRCDAYTTDRSGLAATRAGKASNPDDYV
                     ILPEVISKEPLAPAVRDGDEEWFDVVKWTVYAMIGAEEKGITSATVDEALKSDDPDVK
                     RMLGVSPGLGKALHLDEKWAYNVIKQVGNYGEVFERNVGKQTPLKLDRGLNNLWTQGG
                     LMYAMPFR"
     gene            4355..5524
                     /locus_tag="VGP50_16915"
     CDS             4355..5524
                     /locus_tag="VGP50_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435936.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV8029117.1"
                     /translation="MALRRSDGAWWNDERYRRLLFQVLIIGVVGAILFYLGSNAVANL
                     RRQGIVGGFGFVTHEAGFEISPTLVAYSPADSYARALLVGLLNTLLVAAIGIVFASLL
                     GLVVGLARLSRNWLVGRLALAYVELMRNTPLVLQLFVWWNLLKISAPGPRQAWEVLPG
                     IFVSNRGVALPVPSYAPAHGWCLLALALGLACSLLLARWARRRRALTGKGFPTTRIGL
                     ALTFLPPAMLFLALGAPFSLDMPQLGTFNFSGGITVSPEFSALVVGLVVYTAAFIGEI
                     VRSGVEAVSRGQSEAARALGLRGAQVLRLVVLPQAVRVIIPPLTSEYLNLAKNSTLAV
                     TIGFFDYFSVANTMLNQTGQTIEAMALIAAVYLSLSLGLSLLMNLYNRRMALVER"
     gene            5527..6606
                     /locus_tag="VGP50_16920"
     CDS             5527..6606
                     /locus_tag="VGP50_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV8029118.1"
                     /translation="MAALALSTPLPAARAFRWLRANLFNTRLNTALTLLVLYGLVKLV
                     PPLIDWAIVKASVTAPNEAACAARGGACWAFITTWYRFILFGRFPYDQQWRPAIVVVL
                     LVLVLVASGDRRLWGRGLAVLWLAGLALAGVLMSGGVFGLAPVDTDLWNGLPLTLILA
                     IGGLLLGFPLAVLLALGRRSAMPGVRLLSTVYIEVFRGLPFITVLFMAAVLFPLFLPP
                     GVSIAKLWRVEVAFILFLAAYLAEVVRGGLQAIPRGQYEAAAALGLGYWQEMRLVILP
                     QALRISIPPLVGTFIGGFKDTALVSIVGLFDLLQDVTIANNDPAWRGAFVEGYAFIAV
                     IYFAFCSSMSRYSRSLEARSGGPKR"
     gene            6642..>7253
                     /locus_tag="VGP50_16925"
     CDS             6642..>7253
                     /locus_tag="VGP50_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV8029119.1"
                     /translation="MIELRHVHKWYGEFHVLNDVSLAVRRGERVVVCGPSGSGKSTMI
                     RCINRLEEHQQGEIYVDGIALTGDVKHIEAIRREVGMVFQQFNLFPHLTVLENLTLAP
                     IWVRNTPKHEAEELAMHYLARVRIPDQAGKYPGQLSGGQQQRVAIARSLCMRPQVMLF
                     DEPTSALDPEMIKEVLDVMVGLAQDGMTMVCVTHEMGFAREVAD"
BASE COUNT         1145 a         2420 c         2497 g         1191 t
ORIGIN      
        1 gggaggatgt tgcggatcga gatccccgtc gggcgctcga gcgccagcgc cacgtcgcgg
       61 gcgcggatgc gcgcgcgcac cgccgtgccg agcggcaaag cgacatgggc gacgcgcagc
      121 cggccgccgg gaaaggcgag ctcggtcaag ccgtagctca catcctgtcc ggccaccgtg
      181 gcgcggatca ccgatcccgc ctcgtagcgt cccgtgagcg gccggagatc gatgcggctc
      241 gtcagctcct cgaccgtgcc gattgcggcg acgcgccctt ccgacatcag caccagcatg
      301 tcggcgaggc ggaggatctc ctccatggcg tggctcacat agatcacggg aatgccgagc
      361 tcgctgcgca ggcgctcgat gaacggcagg atctccgcct tgcgcggcgt gtcgagcgcg
      421 gccaggggct cgtccatgag caggagccga ggctccgcga gcagcgcacg gccgatggcg
      481 acacgctgct tctcgccgcc cgagagatcg ccgatgcggc ggtcgagcag cccctcgagg
      541 ccgagcagcg ccacgatcgc atcgaggccc ggcgcgtcgc gcgcggtgcc gtgccgccgg
      601 ccgtatgtca ggttgccgcg cacggagtag tgcgggaaga gacggccttc ctggaagaca
      661 tagccgaggc ggcggcgctc gggtgggagg tcgatgccgt cctcggacga gaacagcacg
      721 tcgccgccga tggcaatgcg gccgcgatcg ggccgcacga gacccgccag catattgacg
      781 agcgtcgtct tgcccgagcc cgagcggcca aagagcgccg tgatcccctg ggcgggtgtg
      841 ttgaagcgcg cggcaagggt gaaggcgccc agccgtttct cgacatcgat ctcgatcatg
      901 cgaggccgcg gatgcggcgg tcgagacggc gcgccatcag ctccgaggcg acgagcgccg
      961 ccatcgccag cagcaccgag aggatggcga ggcgccatgc cgacgcttcg ccgcccggcg
     1021 tctgcgtcag cgcatagagg gcaagcggca gcgtgcgcgt ctcgccgggg atgttggaga
     1081 cgaaggtgat ggtggcgccg aactcgctga tgctgcgcgc gaaggcgagg atcgccccgg
     1141 tgaggatgcc gggcgccatc agcggcagcg tcaccgtggc caggacgcgg agcgggcgag
     1201 cgcccagcgt gcgcgccgcc gcctcgagcc gctggtccac gagctccagc gacagccgca
     1261 tggcacgcac catcagcggg aaagccatga ctccggctgc gagcgccgca cccttccagt
     1321 tgaaggcgaa ggtgatgccg aggctctcat agagccaggc accgaccggg ccgtgacgtc
     1381 cgaacagcac gaggaggcta tagccgacga ccaccggcgg cagcaccagc ggcagatgga
     1441 cgaggccgtt gagcaggatc ttgccgggaa agtcgtgccg cgccagcagc caggcgatgg
     1501 cgaggccgaa ggggagggac acggcgacgc tgacgacacc gacgcgcaag ctcaggagca
     1561 gcgcctcgct ctcgagcggg ctcaacgtca gcaacgtgag ttcccctcgc ccctcgcggg
     1621 agagggaggc gcgcgccgag cgcgcgctcg cgacgacgcc tgcggcgtcg cgcgggggtg
     1681 agggggctcg cgacgagcag cggtgcttgc ggacccctca ccctcccgcc gctgcgcggc
     1741 gggcccctcc ctctcccgca aggggagagg gggatgcgag gcgcttccgc catcgggaat
     1801 catcagcgct gggcgttgag gcgggcgaag ccgtgctcga ggtcggcgat caggtcgcct
     1861 tggtcctcga ggccggcatg gatgcggagc acggggccgg attgccaggg cttggcactg
     1921 cgcagccggt cggggcggac ctggaccatg aggctctcga agccgcccca gctgaagccg
     1981 aggccgaaaa gctccatgcc gtcgagcatg gcgtcgatcg ccggcttggc gatatcggcg
     2041 cgcagcacga tgctgaagag gccgctcgag ccgctgaaat cgcgtttcca gaaatcatgg
     2101 ccggggcagc ccggaagggc cggatgcagc acccgctcga cctccggacg cgcggcgagc
     2161 cagcgtgcga gggcgaggcc ctgaagctcg tgctggcgca agcgaagagc ggcggtgcgc
     2221 aagccgcgca gcgccaggta gcaatcgtcg ggcgccgcgt ggttgccgag cagcgtcgcc
     2281 gtctcgcgca ccggcatgaa gctcgtctcg gtgcagacgg cgatccccat catggcatcg
     2341 gagtggccga cgatgtattt cgtggcggcg atgatcgaga catcgacgcc caacgccagc
     2401 ggcttgagga agagtggcgc cgcccaggta ttgtcgagca tggtgacgat gccgcgcgcc
     2461 ttggcgacgg cgacgatcgc cggcacgtcc tggacttcga aagtgagtga gcccggactc
     2521 tcgaggtaga tgacgcgcgt gttgtcccgg acgagcccgg cgatgccttc gccgatcagc
     2581 ggatcataga aggtgctgtg aacgccgaag cgctcgagga agccgttgca gaagccgcgc
     2641 accgggccat agacgttgtc cggcaccagc acatggtcgc cggctttgag atacgcgagc
     2701 agcgtcgcga agcacgccgc cgcgccggag cagaaggcga cggcgcgatg gccgccctcg
     2761 accgcggcaa cggcatcctc gaaggcgcgc gtcgtcggcg tgccatagcg cccataggcg
     2821 atctcgtcgt aacggttgcg ctgcgccgcc tcgagcgcgg cgacggaggg gtgcaagatg
     2881 gtggaggcgt ggtagaccgg cggattgacg gcgccccggc atgtcgcggg atcgcggccc
     2941 gcatgggtga ggagcgtgtc cggctttttc gatttgctgc gcatcggcgc atcttgcatc
     3001 gccgcgcgcg tggggaaaag ccgcttcctc gccgctcagc attgcgagct ttgacggcac
     3061 catgctacgg tcgcgaccgt atcgctgccg cgtgccagca tcctctggat tggaataatt
     3121 attaaaaatc aatgtggtct aggaggatct cgatggactc agtgagaatg tcgacggcgc
     3181 tcggcgtggc cgtggccgtt gtcgccgcgg ccggtgccgc cgtcgccggc ccgacgttgg
     3241 acagcgtcaa aagccgtggc gtcgtgacct gcgccgtcaa tacggggctt gtggggttcg
     3301 gcatgcccga ccagcaaggg gactacaaag ggctcgacgt cgacacctgt cgcgccatcg
     3361 ccgcggcggt gctgggcgac gcaaagaagg tgaagttcat cccggcgacg acgcagcagc
     3421 gtttcaccgt gctgcagtcg ggcgaagccg acgtgctgac gcgcaacacc acggagacgc
     3481 tgctgcgcga taccgagcaa ggcttcaatt tcgcgccggt cacctattac gacggccagg
     3541 gcttcatggt gccgaagaag ctcggcgtca aaagcgccaa ggagctcaac ggcgccaccg
     3601 tctgcgtcca gcccggcacc accaccgagc tcaacctcgc cgactatttc cgcgcgaaca
     3661 agatgtcgtt caagccgctc accatcgaga agcttgccga ggtcgaggac gcgttcttct
     3721 ccgggcgctg cgatgcctac accaccgacc gctccggcct ggccgcgacg cgcgccggca
     3781 aagcgtcgaa ccccgacgac tatgtgatcc tgccggaggt gatctcgaaa gagccgctgg
     3841 cgccggccgt gcgcgacggc gacgaggaat ggttcgacgt cgtcaaatgg accgtctacg
     3901 ccatgatcgg cgccgaggag aagggcatca cctcggccac tgtcgacgaa gccctgaaga
     3961 gcgacgatcc cgacgtgaag cgcatgctcg gcgtctcgcc tggtctcggc aaggcgctgc
     4021 atctcgacga gaaatgggcc tataacgtga tcaagcaggt cggcaactat ggcgaggtct
     4081 tcgagcgcaa tgtcggcaag cagacgccgc tgaagctcga ccgcggcctc aacaatctct
     4141 ggacccaggg cggcctgatg tacgccatgc ccttccgctg agcggtccct tgcgcgatcg
     4201 aggtagaaag tccttctccg ccctcgaggg cggagaagga tttaggatga ggtgggtgac
     4261 gacgggcgtt gccgaaggca cccccacctc accccagccc tctccgcccc caggggcgga
     4321 gagggagaag aatccgcgtc ggagctgcta ggtcatggcc ctccggcgaa gcgacggcgc
     4381 ctggtggaac gacgagcggt atcgcaggct cttgttccag gtgctgatca tcggcgtcgt
     4441 gggggcgatc ctcttctatc tcggcagcaa cgccgtcgcc aatctgcggc gccaaggcat
     4501 cgtcggcggc ttcggattcg tgacccacga ggccgggttc gagatcagtc cgacgctcgt
     4561 cgcttattcg ccggcggaca gctacgccag ggcgctgctg gtcgggttgc tcaatacgct
     4621 gctggtggcg gcgatcggca tcgtcttcgc gagtttgctc ggcctcgtgg tcggcctggc
     4681 gcggctctcg cggaactggc tcgtgggccg ccttgcgctc gcctatgtcg agctgatgcg
     4741 caacacgccg ctggtgctgc agctcttcgt ctggtggaac ctcctcaaga tcagcgcccc
     4801 cgggccgcgg caggcttggg aagtgctgcc ggggatcttc gtctccaatc gcggcgtcgc
     4861 cttgccggta ccgagctacg ctccagccca tggctggtgc ctcctcgccc ttgccttggg
     4921 cctggcgtgc agccttctgc tggcgcgctg ggcgcgccgc cgccgcgcgc tcacggggaa
     4981 aggatttccc acgacccgta tcggcctggc gctgaccttc ttgccgccgg ccatgctctt
     5041 cctggcgctc ggcgcgccgt tttcgctcga catgccgcag ctcggcacct tcaacttcag
     5101 cggcggcatc accgtgtcgc cggaattctc ggcgctcgtc gtcggtctcg tggtctatac
     5161 ggcggccttc atcggcgaga tcgtgcggag cggcgtcgag gcggtgagtc gcggtcagtc
     5221 ggaagcggcg cgcgcgctcg gcttgcgcgg cgcccaggtg ctgcgcctcg tcgtgctgcc
     5281 gcaggcggtg cgcgtgatca tcccgccgct caccagcgag tatctcaatc tcgccaagaa
     5341 ctccacgctc gccgtcacca tcggcttctt cgactatttc tcggtggcca acaccatgct
     5401 caaccagacc gggcagacga tcgaggcgat ggccctcatc gccgccgtct atctgtcgct
     5461 gagcctgggg ctgtccttgc tcatgaacct ctacaaccgc cgcatggcgc tggtggagcg
     5521 ctgagcatgg ccgcgctcgc gctgtccacg ccactgccgg cggcccgcgc ttttcgctgg
     5581 ctgcgcgcca acctcttcaa tacgcggctc aacacggcac tgacgctgct cgtgctttac
     5641 ggactggtca agctggtgcc gccgctcatc gactgggcga tcgtgaaggc ctcggtcacg
     5701 gcgcccaacg aggccgcctg cgccgccagg ggcggtgcct gctgggcctt catcacgacc
     5761 tggtaccgct tcattctgtt cggccgattc ccctatgacc agcaatggcg gccggcgatc
     5821 gtcgtcgtgc tgctcgtcct ggtccttgtc gcgagcggcg atcgccgcct ctggggccgc
     5881 ggcctcgccg tgctctggct cgccgggctc gcgctggccg gcgtgctcat gtcgggcgga
     5941 gtcttcggcc tcgcgccggt cgacaccgac ctctggaacg gcctgccgct cacgctcatt
     6001 ctggcgatcg gcgggctcct gctgggcttt ccgctcgcgg tgctgctggc cctcggccgg
     6061 cgctcggcga tgccgggcgt gcgccttctc tcgaccgtgt atattgaggt gtttcgcggc
     6121 ttgccgttca tcaccgtgct cttcatggcg gcggtgttgt tcccgctctt cctgccgccc
     6181 ggtgtcagca tcgccaagct gtggcgcgtc gaggtcgcct tcatcctgtt cctcgcggct
     6241 tatctcgccg aggttgtgcg gggcggcctt caggcgattc cgcgcggcca atacgaagcg
     6301 gcggcggcgc tgggcctcgg ctactggcag gagatgcggc tcgtcatcct gccccaggcg
     6361 ctgcgcatct ccatcccgcc gttggtcggc accttcatcg gcggcttcaa ggatacggcg
     6421 ctcgtcagca tcgtcggcct cttcgacctg ctgcaggacg tcaccatcgc caacaacgac
     6481 ccggcctggc gcggcgcctt tgtcgaaggc tatgccttca tcgccgtgat ctatttcgcc
     6541 ttctgctctt ccatgtcgcg ctacagccgg tccctcgaag cgcgaagcgg cgggcccaag
     6601 cggtgagcgc cgagagcaag aatgccgacc ccgatcgcct gatgatcgag cttcgccatg
     6661 tgcataaatg gtatggcgag ttccatgtgc tgaacgatgt cagccttgcc gtgcgccgcg
     6721 gcgagcgcgt cgtcgtctgc ggtccctcgg gctccggcaa atcgacgatg atccgctgca
     6781 tcaaccgcct cgaggagcat cagcagggcg agatctatgt cgacggcatc gcgctgaccg
     6841 gcgacgtcaa gcacatcgag gccatccgcc gcgaagtcgg catggtgttc cagcagttca
     6901 acctcttccc gcatctgacg gtgctggaaa acctgacgct cgcgccgatc tgggtccgga
     6961 acacgccgaa gcacgaggcc gaagagctgg cgatgcatta tctggcgcgc gtgcggattc
     7021 ccgatcaggc cgggaaatat ccgggccagc tctcgggcgg tcagcagcaa cgcgtcgcca
     7081 tcgcccgctc gctgtgcatg cgcccgcagg tgatgctgtt cgacgagccc acctcggcgc
     7141 tcgatcccga gatgatcaag gaagtgctcg acgtcatggt gggtctggcg caggacggca
     7201 tgaccatggt ctgcgtcacc catgagatgg ggttcgcccg agaggtcgcc gac
//
LOCUS       DASYNS010000551         4183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_140731, whole genome shotgun sequence.
ACCESSION   DASYNS010000551 DASYNS010000000
VERSION     DASYNS010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140731"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VGP50_16930"
     CDS             <1..820
                     /locus_tag="VGP50_16930"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8029120.1"
                     /translation="ARPKRRRWLWPPIGLALAYILANCALYGMQRSLVFQPSTGGEPA
                     RADSLGMATVKTTAADGIAVTHWYAPPRGAAAVVVLFHGNGGTVADLEPWAAAFRARG
                     YGVVLADYRGYSGNPGEPSEAGVYADARAILAWLEEQGIAERRIVLLGWSLGSGVAVQ
                     MAVERHPAALVLLAPYTALVDVAATQYPFSPVRSLMIDRFDSMSKIARIDAPLMIVHG
                     EADEVIPTAMGRRLFAAAHEPKRAVYLPGTGHVIPPGRAVADVAAFIDESLPSH"
     gene            852..2006
                     /locus_tag="VGP50_16935"
     CDS             852..2006
                     /locus_tag="VGP50_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8029121.1"
                     /translation="MDFRLTEEQRAIRDMARAFAEREFRPHAARWDEEKHFPVQALRA
                     AASLGFAAIYVREDGGGAGLERIDAALVFEELAAGCVSTAAYLSIHNMASWMIDRFGS
                     AEQRRRFLPKLASMEHFASYCLTEPGAGSDAAALTSRARRDGDHYVIDGTKAFISGGG
                     TSNLYVCMLRTGGEGPGGISAIVVEKGTPGLAFGPNERKLGWNSQPTTLVTFENCRVP
                     VANRLGAEGDGFKIAMMGLDGGRLNIAACSLGGARAALDAALRYAGERIQFGRKLAEF
                     QALQFKLADMATELDAARLMVWRGASSLDDDDPEATLHCAMAKRFATDAGFEVANAAL
                     QLHGGYGYLRDFPLERYVRDLRVHQILEGTNEIMRVVIARRLLAGGNRGP"
     gene            2003..3073
                     /locus_tag="VGP50_16940"
     CDS             2003..3073
                     /locus_tag="VGP50_16940"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV8029122.1"
                     /translation="MSAAEILLERQGALGVVTFNRPKALNTLTLGMYRAFDPRLIAWG
                     EDGAVLALLVRGAGERAFCAGGDVRAVYDARHGGGGAGDYKADFFREEYTLIQRVHSF
                     PKPYIALVDGIAMGGGCGVSINGSHCIATERTLFAMPEVHIGLFPDVGASRFLNLCPG
                     RIGRYLALTGTRLRAADALYCGFASHYVPHDRLADFANTLASLDWSLGDARRQVDRVL
                     ARFAADPGPAELPALQPAIDRCFAGDSVEGIVAALERESEDWAKAALAAMRRASPLSL
                     KITFRQLELGRGMTLEEALTLEYRMTQHVMAGHDFFEGIRALLVDKDQKPRWQHAALE
                     AVSDAEVERYFAELGERELRFA"
     gene            3197..3574
                     /locus_tag="VGP50_16945"
     CDS             3197..3574
                     /locus_tag="VGP50_16945"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HEV8029123.1"
                     /translation="MTENELGEAIIGAAMKVHSALGPGLLESAYELCLAHEIERRGLI
                     VGRQVSIPVRYESLVIENGYRIDLLVDDRVVVELKAIEALLPVHRAQLLSYLRLGGFK
                     LGCLLNFNVAHMRDGIARIVNGL"
     gene            3696..>4183
                     /locus_tag="VGP50_16950"
     CDS             3696..>4183
                     /locus_tag="VGP50_16950"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8029124.1"
                     /translation="MKIGFIGLGNMGGPMAANLVKAGHDVTGFDLAEPALAALAKAGG
                     KRAANVAATVAAADMVLTMLPAGPQVRAAHGGAAGVLAAAKPGALLVDSSTIDVESAR
                     ALIKEAAVRGFDMLDAPVSGGVAGAAAGMLTFMVGGSAAGFERALPVLQAMGKTIVHA
                     GPA"
BASE COUNT          606 a         1383 c         1454 g          740 t
ORIGIN      
        1 tgcgcggccg aagcgccggc gctggctctg gccgccgatc gggctcgccc tcgcctacat
       61 cctcgccaat tgcgccctct acggcatgca gcgctcgctg gtcttccagc cctcgaccgg
      121 cggcgagcct gcccgcgcgg actccctggg catggcgacg gtgaagacga ccgcggccga
      181 cggcattgcc gtgacgcatt ggtacgcccc gccccggggt gccgcggcgg tcgtcgtcct
      241 ctttcacggc aatggcggca ccgtcgccga tctcgagcca tgggcggcgg cgtttcgcgc
      301 gcgcggctat ggcgtggtgc tcgccgatta tcgcggctat agcggcaatc cgggcgagcc
      361 cagcgaagcc ggcgtctatg ccgatgcgcg cgccatcctt gcctggctcg aggagcaggg
      421 catcgccgag cgcaggatcg tgctcctcgg ctggtcgctc ggcagcggtg tcgccgtgca
      481 gatggcggtg gagcgccacc ccgccgcgct cgtgctgctg gcgccctaca cggcgttggt
      541 cgacgtcgcc gccacgcaat atccgttttc tccggtgcgc tcgctgatga tcgatcgctt
      601 cgacagcatg agcaagatcg cccggatcga tgcgccgctc atgatcgtgc atggcgaggc
      661 ggacgaggtg atcccgacgg cgatggggcg ccggctcttc gccgcggcgc atgagcccaa
      721 gcgcgccgtt tacctgcccg ggaccggcca tgtgatcccg cccgggcgcg ccgtcgccga
      781 tgtcgcggcg ttcatcgatg agagcttgcc atcgcattga tgacgcggaa ggccgcgact
      841 atgatcacgg catggatttt cggctcaccg aggagcagcg cgccatccgc gacatggcgc
      901 gcgcattcgc cgaacgcgaa ttccgccctc atgccgcgcg gtgggacgag gagaagcatt
      961 tcccggtcca ggcgcttcgc gcggcggcgt cgctgggctt cgccgccatt tacgtgcgcg
     1021 aggatggggg cggcgccggg ctcgagcgta tcgatgcggc gctcgtcttc gaggaactgg
     1081 cggcgggctg cgtttccacc gcggcctatc tctcgatcca taacatggcg tcgtggatga
     1141 tcgaccgctt cggcagcgcg gagcagcgcc ggcgcttcct gccgaagctc gccagcatgg
     1201 agcatttcgc gagctactgc ctgaccgagc cgggcgctgg ctcggacgcg gcggcgctca
     1261 cgtcgcgggc gcggcgcgac ggcgatcact atgtcatcga cggcacgaag gcgttcatct
     1321 cgggcggcgg gaccagcaat ctctatgtct gcatgctgcg gacgggtggg gaagggccgg
     1381 gcggcatttc cgctatcgtc gtcgagaagg ggacgccggg tctcgccttc ggccccaacg
     1441 agcgcaagct cggctggaac agccagccga cgacgctggt gaccttcgag aattgccgcg
     1501 tgccggtggc gaaccgcctg ggcgccgagg gcgacggctt caagatcgcc atgatgggtc
     1561 tcgatggcgg ccggctcaac atcgcggcat gctcgctcgg tggcgcccgc gcggcccttg
     1621 acgcggcgct gcgttatgcc ggcgagcgca ttcagttcgg ccgcaagctc gccgagttcc
     1681 aggcgttgca gttcaagctg gcggacatgg cgaccgagct cgatgcggca cggctgatgg
     1741 tgtggcgggg cgcctcgagc ctcgatgacg acgatcccga ggcgactctg cattgcgcca
     1801 tggccaagcg cttcgccacc gatgccggct tcgaggtcgc caacgcggcg ctccagctcc
     1861 atggcggcta tggctatctg cgcgacttcc cgctggagcg gtatgtgcgg gatttgcgcg
     1921 tgcatcagat cctcgaaggg accaacgaga tcatgcgcgt ggtgatcgcc cggcgtcttc
     1981 tcgccggcgg caatcgcggt ccatgagcgc cgccgagatt ctgctggagc gtcagggcgc
     2041 gctcggcgtc gtcaccttca atcgtccgaa ggcgctcaac acgctgacgc tcggcatgta
     2101 tcgcgccttc gacccccggc tgatcgcctg gggcgaggat ggcgcggtcc tcgcgcttct
     2161 cgtgcgcggc gcgggggagc gcgccttctg cgccgggggc gacgtgcgcg ccgtctatga
     2221 cgcacgtcat ggcggcggag gcgccggcga ctacaaagcg gatttcttcc gcgaggagta
     2281 caccctcatc cagcgcgtcc acagcttccc caagccctat atcgcgctgg tggacggcat
     2341 cgccatgggc ggcggctgcg gcgtctcgat caacggctcg cactgcatcg cgaccgagcg
     2401 cacgctcttc gcgatgccgg aggtgcatat cgggctcttt cccgatgtcg gcgcgagccg
     2461 gttcctcaat ctctgtcccg ggcgcatcgg ccgttacctc gcgctcaccg gcacaagact
     2521 gcgcgcggcc gacgcgctct attgcggctt cgccagccat tatgtgccgc atgaccggct
     2581 ggccgatttc gcgaacacgc tcgcctctct cgactggtcg ctgggcgacg cacgccgaca
     2641 agtcgatcgc gtgctggcac gcttcgctgc cgatcccggg ccagcggagc tgccggcgct
     2701 tcagccggca atcgatcgct gcttcgcggg cgattcggtc gaggggatcg tcgccgctct
     2761 cgagcgcgag tccgaggatt gggccaaggc ggcactggcg gcaatgcgcc gcgcctcgcc
     2821 cttgagcctc aagatcactt tccgccagct cgagctcggc cgcggcatga cgctcgagga
     2881 ggcgctgacg ctcgaatatc gcatgaccca gcatgtcatg gccgggcatg atttcttcga
     2941 aggcatccgg gcgcttctcg tcgacaagga ccagaagcca cgctggcagc atgccgcgct
     3001 cgaggcggtg agcgacgccg aggtcgagcg ctacttcgcg gagctcggcg agcgcgagct
     3061 cagatttgct taaaccggtg ccctctaacc ggtgcttaac cagaggtctc gtcgttgccc
     3121 agaccattga ctgcggaccg gctccggttc attagcgaac gcggaggccg cggaggtcac
     3181 gcggagggcg cagaggatga ccgagaacga actcggcgaa gccatcattg gcgcggcgat
     3241 gaaggtccac tcggcgctcg gccccggtct gctggagagc gcgtacgagc tttgcctcgc
     3301 ccatgagatc gaacgacgag gtctcattgt gggcagacag gtttccattc ccgttcggta
     3361 cgaaagcctg gtgatcgaaa acggctaccg catcgatctg ttggtcgatg atcgggtggt
     3421 tgtggagctg aaggcgatcg aggcgcttct ccccgttcat cgcgcgcagc tgctcagtta
     3481 tcttcgcctt ggcggcttca agctcggctg tcttctcaat ttcaatgtcg cgcacatgcg
     3541 cgatgggatt gctcggattg tcaatggtct ctgaccggct aaatgttaac cgctttgcta
     3601 gtgaacccag agacgcggag gatgactgct cctcggcgcc ctccgcgcag cctctgcgtt
     3661 catccttttt gaacgggcgc tagggggatg tgaccatgaa gatcggtttt atcgggctcg
     3721 gcaacatggg cgggccgatg gcggccaatc tcgtgaaggc cggccatgac gttaccggtt
     3781 tcgatctcgc cgaaccggcc ctggcggcgc tcgccaaggc cggcggcaag cgggcggcca
     3841 acgtcgccgc gacggtggcg gcggccgata tggtcctcac catgctgccg gcggggccgc
     3901 aagtgcgcgc ggcccatggc ggcgccgcgg gcgtgctcgc ggcggcgaag ccgggcgccc
     3961 ttctcgtcga ttcttcgacc atcgacgtcg agtcggcgcg tgcgctcatc aaggaggcgg
     4021 cggtgcgcgg cttcgacatg ctcgacgcgc cggtgtcggg cggtgtcgcc ggcgcggcgg
     4081 cggggatgct gacattcatg gtgggcggca gcgcggccgg cttcgagcgc gccttgccgg
     4141 tgctgcaggc gatgggcaag accatcgtcc atgcggggcc ggc
//
LOCUS       DASYNS010000552         2867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_452658, whole genome shotgun sequence.
ACCESSION   DASYNS010000552 DASYNS010000000
VERSION     DASYNS010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452658"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..2011
                     /locus_tag="VGP50_16955"
     CDS             236..2011
                     /locus_tag="VGP50_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029125.1"
                     /translation="MRVVFAAEKPLEGELSLIAPDGSVAAKSHERRGGPPYFWYAEVG
                     TPAAGTWHARLARERAPAECSTLTYDIAVLDHEPPRPHGAPGSVWPVRNTWNRATENL
                     FSAWIEKLFDAPLDAAPSWPALHEVLRDRSRNILFNYLGLGEDQMGTVVRPDCADLPY
                     FLRAYFAFKMGLPFGYSKCSRGGGGKPPRCHEWFSIQNSEQARSAPAEPKIASGAPLE
                     MVTQAIGQPAAPPAEKRLGLAGSFGSYLRVLGDGVHSGSARTPLNDESTDNYPVPLKQ
                     ETLRPGTVYADPYGHVLMLVRRVPQTSGAAGVFLAVDGQPDGTVARKRFWRGNFLFAQ
                     DPALGGPGFKRFRPIVTDKSGAERRLTNEEIAKNPQYADFSLDQARLTPDAFYDRMDD
                     VMSPAPLDPMRAMGEAITSLEEQVKARVTSVENGRKYQSSGRGDVDMPNGPSIFETTG
                     AWEDFATPARDLRLLIAIDVVLGFPDRVARRPERYAMPAGKSVADVKAELQNMLASEL
                     ATRKFSYMRTDGSAWTLALKDVAERAPSLEMAYNLNDCVELRWGAAERSEEASTCKRH
                     ASGAQRSKMSEYRAWFHERRRPPRA"
     gene            complement(2046..2816)
                     /locus_tag="VGP50_16960"
     CDS             complement(2046..2816)
                     /locus_tag="VGP50_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029126.1"
                     /translation="MTFAYVSVEEAIERRGVRMVVVGNVPSPWGEAAKGILHIKGIEW
                     AAVRLAYDSEALQQWAGQRNGPVLIYDDERPRSGWAEILLLAERLAPAPPLIPAKADE
                     RALAFGLAHEICGEGGLGWSRRLQLIHAGLNNSGGFPERVSKYLAKKYGYSPAAGAAA
                     GARVVALLTMLAARLKVQQQAGSRYYIGDSLTAVDVYSATFAAMFGPLPHEVCKMDAS
                     TRAAFSARDADIDAALDPIFLAHRDMTYARHLELPLSL"
BASE COUNT          449 a          961 c          990 g          467 t
ORIGIN      
        1 gccatgattc agcccaagct tttgcggtat tctcggctgc ggacagggga agggggctca
       61 attgtctctt gcatatcggc ttgccgctgc gaaggcattg ttgctgaccg caggcgcatt
      121 gttcgttctc gctccctcag cgggggactc gcgggccgcc gctccgggca gctgcgaaga
      181 cgcggccgag ctcgcggtcc tgccctcgcc gattgcgcct tggaagggcg cccctttgcg
      241 cgtcgtcttt gccgcggaga agccgctcga gggcgagctt tcgctgattg cgcccgacgg
      301 cagtgtcgcg gcgaagtcgc acgagcggcg cggcgggccg ccatacttct ggtacgccga
      361 ggtcgggacg ccggcggcag ggacgtggca cgcgaggctc gcgcgcgagc gcgcgccggc
      421 cgagtgcagc acgctcacat acgatatcgc cgtcctcgac catgagccgc cgcggccgca
      481 cggggcgccg gggagcgtgt ggcccgtccg caatacttgg aatcgcgcga cggaaaacct
      541 cttttccgca tggatcgaga agctgttcga cgcgccgctc gacgccgcgc cgtcctggcc
      601 ggcgctgcac gaggtgctgc gcgatcgctc gcgcaatatt ctgttcaact atctcggtct
      661 cggcgaggac cagatgggga ccgtcgtccg ccccgactgc gccgacctgc cgtacttcct
      721 gcgcgcgtac tttgcgttca agatggggct gccgttcggg tactcgaagt gctcgcgcgg
      781 cggcggcggc aagccgccgc ggtgccacga gtggttcagc atccagaatt ccgagcaggc
      841 gcgctccgcc ccggccgaac cgaaaatcgc gtctggcgct ccgttggaga tggttaccca
      901 ggccatcggc cagccggccg cgccgccggc ggagaagcgg ctcgggcttg cggggtcgtt
      961 cggcagctat ttgcgcgtgc tcggcgacgg cgtccattcc gggtccgcgc ggacgccgct
     1021 gaacgacgag agcaccgaca actatccggt gccgctgaag caggagacgc tgcggccggg
     1081 gaccgtgtac gccgatccgt acgggcacgt gttgatgctg gtgcggcgcg tcccacagac
     1141 gagcggtgcc gcgggcgttt ttctcgccgt cgacggacag cccgacggca cggttgcgcg
     1201 caagcgcttc tggcgcggca acttcctgtt cgcgcaggac cccgcgctcg gcggccccgg
     1261 cttcaagcgc ttccggccga tcgtcaccga caagagcggc gccgagcggc ggctgaccaa
     1321 cgaggaaatc gccaagaacc cgcaatacgc ggatttttcc cttgatcagg cgcggctcac
     1381 gcccgacgcc ttttacgacc ggatggacga cgtgatgtcg ccggcgccgc tcgatccgat
     1441 gcgcgcgatg ggcgaggcga tcacatcgct cgaggagcag gtgaaagcgc gcgtgacctc
     1501 ggttgaaaac ggacgaaaat accagagcag cgggcgcggc gacgtcgaca tgccgaacgg
     1561 cccttcgatc ttcgagacga ccggcgcctg ggaggatttc gcgacgccgg cgcgcgacct
     1621 gcgcctgttg atcgcgatcg atgtcgtgct cgggttcccg gatcgcgtgg cgcggcggcc
     1681 cgagcgctac gccatgccgg cgggcaagag cgtcgccgac gtgaaggccg aattgcagaa
     1741 tatgctcgcg tccgaactgg cgacccgcaa gttctcatac atgcgcaccg acggctccgc
     1801 ctggacgctg gcgctcaagg acgtggccga gcgcgctcca tctctcgaga tggcgtacaa
     1861 tctcaacgac tgcgtcgagc tgcgctgggg cgcagcggag cggagcgagg aggcgtcgac
     1921 ctgcaagcgg cacgcctcag gggcgcagcg ctcgaagatg tccgagtacc gcgcctggtt
     1981 ccacgagcgg cggcggccgc cgcgcgcata ggatcgtcag cgcgcgtcgc gagcatcgcc
     2041 gtcaatcaaa gcgagagcgg cagctccagg tgccgggcgt acgtcatgtc ccggtgcgca
     2101 agaaaaatcg gatccagtgc cgcgtcgata tcggcgtccc gcgcactgaa cgccgccctc
     2161 gtgctggcgt ccatcttgca gacctcgtgc ggcagcgggc cgaacatggc ggcgaaggtc
     2221 gcgctgtaga cgtcgaccgc ggtcaatgaa tcgccgatgt agtagcggct tccggcctgc
     2281 tgctgcactt tgaggcgcgc agcgagcatg gtcagcagcg ccacaacccg cgcgccggca
     2341 gcggctcccg ccgcggggct gtagccgtac ttcttggcca gatatttgga cacgcgctcg
     2401 ggaaacccgc cggaattgtt taggccggca tggatcagct gcaggcggcg cgaccagccc
     2461 agtcctcctt cgccgcagat ttcgtgggca aggccgaaag ccagcgcccg ctcgtccgcc
     2521 ttggcgggga tgagcggcgg cgccggcgcc aggcgttcgg cgaggaggag aatttcggcc
     2581 cagccggagc gcggccgttc gtcgtcgtag atcagcactg gtccgttgcg ctggccggcc
     2641 cactgctgca gggcttcgct gtcataggcg agccgcacgg cggcccattc gatgcccttg
     2701 atgtggagga tgcccttggc cgcttcgccc caggggcttg gcacattgcc gaccaccacc
     2761 atgcgcacgc cgcggcgctc gattgcttct tccacgctca cgtatgcgaa tgtcatcgtc
     2821 gtctccggcg cgcgctgatc gaaacgttac cgccaatcgt ccaaaac
//
LOCUS       DASYNS010000553         5215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_452733, whole genome shotgun sequence.
ACCESSION   DASYNS010000553 DASYNS010000000
VERSION     DASYNS010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452733"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VGP50_16965"
     CDS             complement(<1..577)
                     /locus_tag="VGP50_16965"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8029127.1"
                     /translation="MDLELSDEQKMIVATVRRFVREEILPLEAKLDPDASELPEEDFQ
                     RLSAKVKTMGFWGIDIPEEYGGPGIDLETRTLMAIEMAQHRAGLYAPCYGVFGGAGLA
                     QLYEASEEQKQRYLYPTLRGEKHGFFGLTEPSGGSDPARAIETRAVKDGDDWVLNGAK
                     IFISGADKADYGLVFARTDKSKGRDGVTCFIV"
     gene            complement(558..926)
                     /locus_tag="VGP50_16970"
     CDS             complement(558..926)
                     /locus_tag="VGP50_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029128.1"
                     /translation="MAIRLTKPWQELTADAIRALPGQLGVYQLADGEGRVLYIGYAGG
                     RSLFGLRSAIEEARARLGASARRFRYEVNQQYMSRYEELLMLHVADHGALPPETRDAP
                     RKLGRLSPVAAGKDDGSRAQ"
     gene            complement(911..1372)
                     /locus_tag="VGP50_16975"
     CDS             complement(911..1372)
                     /locus_tag="VGP50_16975"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8029129.1"
                     /translation="MRIGRRLGSALLLAALLSAGPAALAQKQYGPGVSDGEIRIGQTM
                     PYSGPASAYGTIGRAELAYFAGINRSGGVNGRKITLLSPDDSLSPPKTVEQTRKLVEQ
                     ENVQLMFSTLGTPTSMGGGEFSATAPTSPSPLEGEGGVGGKQPARRSWPSA"
     gene            complement(1568..2260)
                     /locus_tag="VGP50_16980"
     CDS             complement(1568..2260)
                     /locus_tag="VGP50_16980"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEV8029130.1"
                     /translation="MSMDQRPEDKRASDSGYQGAKPAAATADLWRLFDGADMGAIRDA
                     MSARLAENWWAIALRGVFAILFGAIALVLPGVTLTALVLLFAAYMLVDGVFAIVAAVR
                     AARQHERWGWLVLEGIVDLIAGAIAVVWPLITIVAFVLLMGAWAIVSGGLLVAAALRL
                     HATHGRWLMALAGVVSVIWGALLLLWPIIGAVVLTLWMGAYAFVFGILLLILAFRLRA
                     RRSHSQALQQGA"
     gene            2486..3670
                     /gene="folP"
                     /locus_tag="VGP50_16985"
     CDS             2486..3670
                     /gene="folP"
                     /locus_tag="VGP50_16985"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008946289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV8029131.1"
                     /translation="MTADTVADRLDAPTGGDVSYRQLETLTDIAPDGLWLRPTGLLSG
                     AMVGPARRQAMALPLAGGPLAFSAVELLARDPAGQVIGAITPLAELLRWSERAPVTLT
                     ARIEQLLDRLSAPRPSWAGLPLDRPLIMGIVNVTPDSFSDGGAFIAAEAAIAHGRALL
                     AEGADILDVGGESVRPGATPVAPEDEIARVAPVLRGLAGAGAVLSIDTRHARVMETAL
                     AAGARIINDVSALAGDAQSLAVATATRAPVGLMHMQGEPRTMQSDPRYRLASLDVLEY
                     LAERIAVCARAGIPRERIVVDPGIGFGKRARHNLEIMARLSLFHALGCGVLLGISRKS
                     LIGRIGGALDPKQRLPGSLAGGVHALGQGVQILRVHDVAETRQAAASWQAIAAGDQSQ
                     DW"
     gene            3676..4431
                     /locus_tag="VGP50_16990"
     CDS             3676..4431
                     /locus_tag="VGP50_16990"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV8029132.1"
                     /translation="MPGKLAAALALALVVARPVAAADLTADEVRAALAEHHALAGKNL
                     TELDLSGFDFSHTDLNHADLFGAKLVGAKFVGANLSGARLDLAWIMRADFTDADLSGA
                     SVFGPVVSTTLDTPPPSEAPHFTRANLSGARVIARLTGVDMKGTRFVGARLGVDLKNQ
                     PMGQMRNDFSGADLSGADFSHADLNRAELAFAKLAGANLTGANLFRADLANADLSGAD
                     LSGADLTEADLNGTILTDAKGLDSAKGADTGRR"
     gene            complement(4428..>5215)
                     /locus_tag="VGP50_16995"
     CDS             complement(4428..>5215)
                     /locus_tag="VGP50_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014188969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV8029133.1"
                     /translation="MATLTPILRARIAEKRYPGIGGAPAHLALRELVVSVAAGEFVAL
                     VGPSGCGKTTFLNIVAGLDGDFTGELALLPAENGRAPRIGYVFQNPRLLPWRTVRENL
                     MLVLRPGQDPAIVDRLLRAVELEAARDVYPGRLSGGMSRRAAIARAFAVDPDLLLMDE
                     PFVSLDEDSAGRLRQLLLELWRARPTTVLFVTHDLREAVFLADRLLLLSPAPGRVVAE
                     VRVPLSRAERSDRAATDRLYHEIGARHRRLVEDAIGGAMRSSL"
BASE COUNT          843 a         1864 c         1753 g          755 t
ORIGIN      
        1 cgacgatgaa gcaggtgacg ccgtcgcggc ccttgctctt gtcggtgcgc gcgaaaacga
       61 ggccgtaatc cgccttgtcg gcaccgctga tgaagatctt ggcgccattc agcacccaat
      121 cgtcaccgtc cttcaccgcg cgcgtctcga tggcgcgcgc gggatcgctg ccgcccgagg
      181 gctcggtgag gccgaagaag ccgtgcttct caccgcgcag cgtcggatag agatagcgct
      241 gcttctgctc ctcgctggct tcgtagagct gcgccaggcc cgcgccacca aagacgccgt
      301 agcagggcgc atagagcccg gcgcgatgct gcgccatttc gatggccatg agcgtgcgcg
      361 tctcgagatc gatgccgggc ccgccatatt cctcgggaat gtcgatgccc cagaagccca
      421 tcgtcttgac cttggcggag agacgctgga aatcctcctc cggcagctcg ctcgcatcgg
      481 gatcgagctt cgcttcgagc ggcaggatct cctcgcgcac gaagcgccgc acggtggcga
      541 cgatcatctt ctgttcgtca ctgagctcga gatccatcgt ccttccctgc cgcgaccggg
      601 ctcaagcgcc cgagcttgcg cggcgcatcg cgagtctcgg gcggcagcgc gccgtgatcg
      661 gcgacgtgca gcatcaggag ctcttcatag cggctcatgt attgctggtt cacctcgtag
      721 cggaagcgcc gggcggaagc gccaagccgc gccctcgcct cctcgatggc gctgcgcagg
      781 ccgaagagcg agcgcccgcc agcatagccg atatagagga cgcggccctc gccgtcagcg
      841 agctgataga cgccgagctg gcccggcagc gcacggatcg catccgccgt cagctcctgc
      901 cacggcttgg tcaggcggat ggccatgacc gccgtgccgg ttgcttaccc cccacccctc
      961 cctccccctc aaggggggag ggggaagttg gtgccgtggc agagaattcc cctcccccca
     1021 tgctggtggg cgtgccgagc gtgctgaaca tcagctgcac attctcctgc tcgaccagct
     1081 ttcgcgtctg ctcgacagtc ttgggcgggc tcagggagtc gtcgggagag agcagcgtga
     1141 tctttcgccc gttgacgccg cccgaccggt tgatcccggc gaaataggcg agctccgcgc
     1201 ggccgatcgt gccataggcc gaagcggggc cgctatacgg catggtctgg ccgattctga
     1261 tctcgccgtc gctgacgccg gggccgtatt gcttctgcgc gagcgcagca gggccggcgg
     1321 agagaagcgc ggcgagcaaa agcgccgagc ccagccgccg cccgatccgc atcacgccct
     1381 ccccgcttgg gccgctttta ccttgagata cggcccattg cccgtattcg caggacaagc
     1441 gtagtcccgc agaaacgatg ccgtcaacga cgcggacctg cgccaatgtc aatggcgcca
     1501 taccggcgaa gagccgggcg ctctcgccga gaagcggccg gcaagcgtca agggcgaatg
     1561 agcggcgtca ggcgccttgc tgaagcgcct gggaatggct gcgtcttgcg cgcaagcgga
     1621 aggcgaggat gagcaacagg atgccgaaga cgaaggcata agcgcccatc cacaaggtca
     1681 gcacgacggc accgatgatg ggccagagaa ggagcagggc gccccagatc acggagacga
     1741 cgccggcaag cgccatcagc catcgtccat gcgtcgcgtg aagccgcagg gcggcagcga
     1801 cgagaagtcc gccgctgacg atcgcccagg cgcccatcag cagcacgaag gcgacgatgg
     1861 tgatgagcgg ccagaccacg gcaatcgcgc cggcgatgag atcgacgatc ccttcgagga
     1921 cgagccagcc ccagcgctcg tgctgccgcg ccgcgcgcac cgccgcgacg atggcgaaga
     1981 ccccatccac cagcatgtag gcggcgaaca gcagcaccag cgcagtcaag gtcacgccgg
     2041 gcagcaccag cgcgatcgcg ccgaacagga tagcgaagac gccgcgtagc gcgatcgccc
     2101 accaattctc tgccaaccgc gcactcatcg cgtcgcggat ggcgcccata tcggcaccat
     2161 cgaagaggcg ccacaggtcc gccgtcgcgg cggcgggctt ggcgccctga tagcccgagt
     2221 cggatgcgcg tttgtcttca ggacgctgat ccatgctcat gccgttcctc cagcggttgt
     2281 gcgtgggtgg gcctcgcaaa cgaatgcgat ctccccaatc tctataaacg cccgccccgc
     2341 cgcttcgctt caaccaaccc ggccaaagat gaacgcgacg acgtcgccgg tctctcgggc
     2401 ggcttttcgc cggggtgagc gccggagaca cgcgtttgcc ggcggttcct gccggtggca
     2461 caatgtcgct catgagggct ccctggtgac cgccgacacc gttgccgatc gacttgacgc
     2521 gccgaccggc ggagacgtgt cctatcggca gctcgagacg ctgacggaca tcgcgcccga
     2581 cgggctgtgg ctgcggccca ccgggcttct gagcggcgcg atggtcggcc cggcgcgacg
     2641 ccaggcgatg gccctgccgc tggcgggcgg gcccctcgcc ttcagcgccg tcgagctcct
     2701 ggcccgcgac ccggcgggac aggtgatcgg cgcgatcacg ccgctcgcgg agctgctgcg
     2761 gtggtcggag cgcgcgcccg tgacgttgac ggcgcgcatc gagcagcttc tcgaccggct
     2821 gagcgcgccg cgccccagct gggccgggct tcctctcgat cgtccgctga tcatgggcat
     2881 cgtcaatgtc acgcccgaca gcttctctga cggcggtgcc ttcatcgcgg ccgaggcggc
     2941 gatcgcgcat ggccgtgcgc ttctagccga aggcgccgac atcctcgacg tcggcggaga
     3001 gtcggtgcgg cctggtgcca cacccgttgc gcccgaggac gagatcgccc gcgtcgcgcc
     3061 ggtgctgcgc gggctcgccg gagccggcgc cgtcctctcc atcgacacac ggcatgcgcg
     3121 cgtcatggag accgcgctcg ccgccggcgc gcgcatcatc aacgacgtgt cggcgctcgc
     3181 cggcgacgcg caaagcctgg cggtggcgac tgccacgcgg gcgcccgtcg ggctcatgca
     3241 tatgcaaggc gagccgcgga cgatgcagag cgacccgcgc taccgcctgg cctcgctcga
     3301 tgtcctcgag tatctggcgg agcggatcgc cgtctgcgcc cgcgccggga tcccgcgcga
     3361 gcgcatcgtc gtcgatcccg gcatcggctt cggcaagcgc gcgcggcaca atctcgagat
     3421 catggcgagg ctcagcctct tccatgcgct gggctgcggc gtgctgctgg gcatctcgcg
     3481 caaatcgctc atcggccgca tcggcggcgc gctcgacccg aagcagcgcc tccccggctc
     3541 gctcgccggc ggcgtccacg cgctgggcca gggtgtgcag atcctgcgcg tgcacgacgt
     3601 cgccgagacc cgccaggccg ccgcgagctg gcaagcgatc gccgcgggcg accaatcgca
     3661 ggactggtga attccatgcc gggaaagctc gccgccgcgc tcgccctcgc tctggtcgtc
     3721 gcgcgccccg tcgccgccgc cgatctcacc gccgacgagg tgcgggcggc gcttgccgag
     3781 caccacgcgc tcgccggcaa gaatctcacc gagctcgacc tgagcgggtt cgacttcagc
     3841 cataccgatc tgaaccatgc cgatctcttc ggcgcgaagc tcgtcggcgc gaaattcgtt
     3901 ggcgccaatc tgagcggcgc gcggctcgat ctcgcctgga tcatgcgtgc cgatttcacc
     3961 gacgccgatc tctcgggcgc gagcgtcttc gggcctgtgg tgtcgacgac cctggacacg
     4021 ccgccgccca gcgaggcgcc gcacttcacg cgcgcgaacc tgtcgggcgc gcgcgtcatc
     4081 gcccgtctca ccggcgtcga catgaaggga acgcggttcg tcggcgcgcg cctcggcgtc
     4141 gatctcaaga accagccgat gggccagatg cgcaacgatt tctccggcgc cgacctcagc
     4201 ggtgccgact tctcccacgc cgacctcaac cgcgcggaac tcgccttcgc caagctcgcg
     4261 ggcgccaatc tcacgggcgc caacctcttc cgcgccgacc tcgccaacgc ggatctcagc
     4321 ggcgccgacc tcagcggcgc cgacctcacg gaagccgacc tcaacggcac catcctcacg
     4381 gacgccaagg gcctcgattc ggcgaaaggc gcggacacgg gacgacgcta aagtgaggag
     4441 cgcatagcgc cgccgatggc gtcctcgacg aggcggcggt gacgcgcgcc gatctcgtga
     4501 tagaggcggt cggtggcggc gcgatcgctc cgttccgcgc gcgacagcgg cacccgcacc
     4561 tcggccacca cgcggccggg cgccggcgag agcagcagca ggcgatcggc gaggaacacg
     4621 gcctcgcgca gatcatgcgt gacgaacagc accgtcgtcg gccgcgcacg ccacagctcg
     4681 agtagaagct ggcgcagccg cccggcgctg tcctcgtcga gcgagacgaa gggctcgtcc
     4741 atcagcagca gatcgggatc gaccgcgaag gcgcgggcaa tggcggcgcg ccggctcatg
     4801 ccgcccgaga ggcgccccgg atagacgtcg cgcgcggcct cgagctcgac cgcgcgtagc
     4861 aggcgatcga cgatcgccgg atcctggccg ggccgcagca ccagcatgag attctcgcgc
     4921 acggtacgcc agggcaggag tcgtggattc tggaagacat agccgatgcg cggcgcgcgt
     4981 ccgttctcgg ccggcaacag cgcgagttcg cccgtgaaat cgccgtcgag cccggcgacg
     5041 atgttgagga aggtcgtctt gccgcacccg gagggaccga ccagcgccac gaactcgccg
     5101 gcggcgacgc tcacgacgag ctccctgagc gccagatgcg ccggcgcgcc gccgatcccg
     5161 ggatagcgct tctcggcgat ccgcgcgcga aggatcggcg tcagcgtcgc catcg
//
LOCUS       DASYNS010000554         3608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4199806, whole genome shotgun sequence.
ACCESSION   DASYNS010000554 DASYNS010000000
VERSION     DASYNS010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4199806"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1419)
                     /locus_tag="VGP50_17000"
     CDS             complement(82..1419)
                     /locus_tag="VGP50_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8029134.1"
                     /translation="MRLKSMGLAAVAALAAGSFCAGEALAQSEQFIPRLVYRTGPYAP
                     NGIPFADGYADYLDMVNARDGGVGSIKIAYEECEDGYNNDKGVECYERLKAHAPTGAA
                     VIDPLSTGISYALIERATADKIPLVTVGYGRADASYGPVFPYVFPFPATYWDSADAMV
                     TYVGQQEGGLAKLKGKKIALVYHDSAYGKEPIATLERLAKENGFEFDKYAVSPPGIDQ
                     KAVWLQIRQKRPSWVLMWGWGVMNSTAVKEAAAIGYPMDHFIGVWYSGAEPDVVPAGK
                     AAVGYKATSFHGVGPDYAVIKDILKYVYDKGGGHTTKEKVGEVLYDRGVINAMYDLEA
                     IRTAQDHFGKTPLWGEQVQWGYEHLDVTPERLATLGFEGLASPLALSCEDHKGNGNVR
                     IIQWDGEHWQTASDWIQPHNEVLRQQYKESALKYAQEKGITPRNCAAQASAAK"
     gene            complement(1511..2590)
                     /locus_tag="VGP50_17005"
     CDS             complement(1511..2590)
                     /locus_tag="VGP50_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8029135.1"
                     /translation="MLYREAGQFKTSYTADQAVFPIRQDRFFIVAVLIAAYVAVPLLG
                     SDYWLLTIMIPFLTFSLAALGLNILTGYAGQVSLGTGGFMAVGAFATYKITTAFPWLN
                     IVVVFLLAGLIAALVGIAFGFPSLRIKGFYLAVATLAAQFFLIWMFNKFPWFTNDTAS
                     GVISAPPRAVFGNFFVTGAATKPVDKYLFALTFVSLLAFAAKNLVRGRIGRAWMAIRD
                     MDIAAEIIGIRPLRTKLLAFAVSSFYCGIAGALWAFIYTGAVEPLAFDIDRSFQILFM
                     IIIGGLGSILGSFLGAAFIVLLPILLDNAPRLAGFAIPVNIVSNLQSMTFGALIIFFL
                     IVEPRGLARLWQIAKDKLRMWPFPY"
     gene            complement(2594..3529)
                     /locus_tag="VGP50_17010"
     CDS             complement(2594..3529)
                     /locus_tag="VGP50_17010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV8029136.1"
                     /translation="MDFLQAIFVTPFASMWDNPAFLLEVIIGGLLTGVMYSLVALGFV
                     LIYKASGVFNFAQGTMALFAALTFVGLLERGAPVWAAFIIAALVMVVMAFLIERLVLR
                     PLVNQEQIILFMATIGLTFFLDGFGQLVWGGDVHPLDIGIPKDPLIFGGVLINEFDLI
                     AAAVCGTMVAALAVFFQKTAIGRALRAVADDHQAALSVGIPLKTIWFIVWSVAGIVGL
                     VAGIMWGSNLGVQFSISLVALKALPVLILGGFTSVPGAIVGGLIIGAGEKVAEVFWGP
                     ATGGAIEDWFAYVLALAFLLFRPQGLFGERIIERV"
BASE COUNT          697 a         1108 c         1228 g          575 t
ORIGIN      
        1 cctctcccgc gacgccggag gtgtcgtcgc gggaggggct gagacgcgcg gcgcccacga
       61 cgcttctgcg cgtcacggac cttacttcgc ggccgacgct tgcgctgcgc agttgcgggg
      121 cgtgatgccc ttctcctgcg cgtatttcag cgccgactcc ttgtattgct ggcgcagcac
      181 ctcgttgtgc ggctggatcc agtcggaggc ggtctgccag tgctcgccat cccattggat
      241 gatgcgcaca ttgccgttgc ccttgtggtc ctcgcagctc aaggccaagg gactggcaag
      301 cccctcgaag ccgagcgtcg ccaggcgctc cggcgtcacg tcgagatgct catagcccca
      361 ctggacctgc tcgccccaga gcggcgtctt gccgaaatgg tcctgcgccg tgcggatcgc
      421 ttcgaggtcg tacatggcgt tgatcacgcc gcggtcataa aggacctcgc ccaccttttc
      481 cttggtggtg tggccgccgc ccttgtcata gacgtatttg aggatgtcct tgatcacggc
      541 gtaatcgggg ccgacgccgt ggaagctggt cgccttgtag ccgaccgctg ccttgccggc
      601 cgggacgaca tcgggctcgg cgcccgagta ccagacgccg atgaagtgat ccatcggata
      661 gccgatcgcc gccgcttcct tgaccgcggt cgagttcatg acgccccagc cccacatgag
      721 cacccagctc gggcgcttct ggcggatctg cagccacacc gccttctggt cgatgcccgg
      781 cggcgagacg gcgtatttgt cgaattcgaa gccgttctcc ttggcgaggc gctcgagcgt
      841 cgcgatcggt tccttgccat aggcgctgtc gtgatagacg agcgcgatct tcttgccttt
      901 gagcttggcc aggccgcctt cctgctggcc gacatacgtc accatggcgt cggccgaatc
      961 ccaatacgtg gccgggaagg ggaagacata ggggaagacc ggcccgtagc tggcatcggc
     1021 gcggccatag cccacggtca ccagcgggat cttgtcggcg gtggcgcgct cgatcagcgc
     1081 atagctgatg ccggtcgaga gcggatcgat caccgccgcg ccggtcggcg catgggcctt
     1141 caggcgctca tagcattcga cgcccttgtc gttgttgtag ccgtcctcgc attcctcata
     1201 ggcgatcttg atgctgccga cgccgccatc gcgggcattg accatgtcga gatagtcggc
     1261 atagccgtcg gcgaagggaa tgccgttggg cgcgtaaggc ccggtgcggt agacgaggcg
     1321 cggaatgaat tgctcgctct gggccagcgc ttcgccggcg cagaagctcc ctgcggctaa
     1381 ggcggcgaca gccgccaggc ccatgctttt gagacgcatc gaaccctccc gttcctgtga
     1441 attcgtaact ttatgctttc cacattaacc ccggcgcggc ggcggcgcaa acgcgcggcg
     1501 cgcgatccca tcaataggga aagggccaca tcctcagctt atctttcgcg atctgccaca
     1561 gacgtgcgag accgcgcggc tcgacgatca ggaagaagat gatgagcgcg ccgaaggtca
     1621 tcgactgcag attgctcacg atgttgacgg ggatcgcgaa gccggcgagc cgcggcgcgt
     1681 tgtcgagcag gatcggcagc agcacgatga aggccgcgcc gagaaaggat ccgaggatgc
     1741 tccccaagcc gccgatgatg atcatgaaaa ggatctggaa ggagcggtcg atgtcgaagg
     1801 cgagcggctc gacggcgccc gtatagatga aggcccagag cgcgccggcg atgccgcaat
     1861 agaaggagct gacggcgaag gcgagaagct tggtgcggag cgggcggatg ccgatgatct
     1921 cggcggcaat gtccatgtcg cggatcgcca tccaggcgcg gccgatgcgc ccgcgcacca
     1981 ggttcttggc ggcgaaagcg aggagcgaca cgaaggtcag cgcgaaaaga tatttgtcca
     2041 ccggcttggt cgcggcgccg gtgacgaaga agttgccgaa gacggcgcgg ggcggcgcgc
     2101 tgatcacgcc cgaggcggta tcgttggtga accacgggaa tttgttgaac atccagatca
     2161 ggaagaactg cgcggcgagt gtcgcgaccg cgagataaaa gcccttgatg cggagactgg
     2221 gaaagccgaa ggcgatgccg acgagcgcgg cgatgagccc ggccagcaga aagacgacga
     2281 cgatgttgag ccaggggaat gcggtggtga tcttgtaggt ggcgaaggcg ccgaccgcca
     2341 tgaagccgcc cgtgccgagc gacacctgtc cggcataacc cgtcaggatg ttgaggccga
     2401 gcgccgccag cgagaaagtg aggaagggga tcatgatggt gaggagccag tagtcgctcc
     2461 cgaggagcgg caccgcgaca taggccgcga tcaataccgc gacgatgaag aaccggtcct
     2521 ggcggatcgg gaagactgcc tggtcggcgg tatagctggt cttgaactgt ccggcctcgc
     2581 ggtagagcat cgcctagacc cgctcgatga tgcgctcgcc gaagaggccc tgcggccgga
     2641 acagcaggaa ggccagcgcc agcacatagg cgaaccagtc ctcgatggcg ccgccggttg
     2701 ccggtcccca gaacacttcc gccactttct cgccggcgcc gatgatcagg ccgccgacga
     2761 tggcgcccgg cacggaggta aagccgccga ggatcagcac cggcagtgcc ttgagcgcca
     2821 ccagcgagat cgagaactgc acgccgagat tgctgcccca catgatgccg gcgacgaggc
     2881 cgacgatgcc ggcaacggac cagacgatga accagatcgt cttcaaggga atgccgacgg
     2941 agagcgccgc ctgatggtcg tcggcgacgg cgcggagcgc gcggccgatc gccgtcttct
     3001 ggaagaagac cgccagcgcg gcgaccatcg tgccgcagac ggcggcggcg atgaggtcga
     3061 actcgttgat gagcacgccg ccgaagatga gcggatcctt gggaatgccg atgtcgagcg
     3121 ggtggacgtc gccgccccag acgagctggc cgaagccgtc gagaaagaag gtgaggccga
     3181 tcgtcgccat gaacaggatg atctgctcct ggttgacgag cggacgcagc acgaggcgct
     3241 cgatcaggaa agccatcacc accatgacca gggcggcgat gatgaaggcg gcccagaccg
     3301 gcgcgccgcg ctccaagagg ccgacgaagg tgagcgctgc aaagagcgcc atcgtccctt
     3361 gcgcgaaatt gaagacgccc gaggccttgt agatgagcac gaatccgagc gccaccagcg
     3421 aatacatgac gccggtgagg agaccgccga tgatcacctc gagcaggaac gcgggattgt
     3481 cccacatgct ggcgaagggc gtgacgaaga tcgcctggag aaagtccatc agccaccgtc
     3541 cccgtcacgc cgaaataccc ctctcccctt gtgggagagg gaggggcccg ccgaaggcgg
     3601 gagggtga
//
LOCUS       DASYNS010000555         5665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3108537, whole genome shotgun sequence.
ACCESSION   DASYNS010000555 DASYNS010000000
VERSION     DASYNS010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3108537"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VGP50_17015"
     CDS             <1..592
                     /locus_tag="VGP50_17015"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEV8029137.1"
                     /translation="RTVMFASALPGEGKSVSSMALALLMANIGQRAIIVDCDTRRPSV
                     HTTFGLSRSDGLTEYLEGAPLGCVVRTVGSPALSVITAGHPVSHPAALFASARMKQLL
                     ADLTNSYDLVVVDSCPVLTSADALVLAPLVDKTVFLVQWGRTPQKAAERALELLREAG
                     ADVAGTMLSMVNIQKMMTHDLGAGSYRKVRRYYQTT"
     gene            666..1826
                     /locus_tag="VGP50_17020"
     CDS             666..1826
                     /locus_tag="VGP50_17020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV8029138.1"
                     /translation="MKVAIIHYWLVGMRGGEKVVEALCDMFPTADVYTHVYRPERISE
                     SIRRHSVKTTFIARLPFSRRHYQKYLPLMPLALEQLDLQDYDLVISSESGPAKGVITR
                     PDALHVCYCHTPMRYVWSGYHSYRGRSNPLTKAVMPALMHRLRMWDLASAARVDHFIA
                     NSHNVAERIKKYYRRDASVIYPPVEMQGVDLHRRTEDFYLFVGQLVPYKGADVAIAAF
                     NRMGKRLVVIGHGEDYQRLRKMSGPTVELVGWQDAATLQDYYARCKALVFVADEDFGM
                     VPVEAMSAGRPVIAYGRGGALETVLPGKTGVLFESQTVDSLISAVDHFESVADTFDRT
                     RIAAHAAAFSKTVFNAQMAETLGRWLDEHGRAQRRALPPLESVADKVAAAAA"
     gene            1848..2972
                     /locus_tag="VGP50_17025"
     CDS             1848..2972
                     /locus_tag="VGP50_17025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV8029139.1"
                     /translation="MRVAIVTRYLKKQDGQGRVNFELAQELLCRGHSVLLVASEVEPS
                     LLEIRGAEWARIPVPPVPTYLLREQIFAVLSSAVLAREHRRLQAIVVNGFATWWRSDV
                     NIVNFVHHAWLKSPLHPARGRLGLAALYRWFYTAVNAKAERAAFAQARSVVAISEIVR
                     RQVVDCGVDADRITVIPGGVDLLAFSPGTGRRSDFGLPEDLFIGLFAGDLRTTRKNLD
                     SVLKAMTRVPDMHLAVAGDTAKSPYPAMARSLGIAGRVHFVGMSTELGALMRAVDAFV
                     FPTRFEPFGLVLLEASACGLPIVTSRAAGACESLDGDGMIVLDDPDDVDGLAAALAML
                     ASDTALRRRMGRSARVAAERLSWKSAVARQARLIEAVARV"
     gene            2984..4174
                     /locus_tag="VGP50_17030"
     CDS             2984..4174
                     /locus_tag="VGP50_17030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV8029140.1"
                     /translation="MTTLAATVPAPITAGKRALSLLQIGAGWFPEFCAGGENVFYHLA
                     KHLPDAGVAVRGLVVGSANVFAQSAGRVLGFAPRSAPLATRLWRARQAIVASFAAGAP
                     DLVATHLALFALPALDKLRSRPLIVHFHGPWAAESALEGAGWMSVGSKRAIERLVYNR
                     ADRVIVLSRAFGEVLRRAYGVDAARITVIPGGVDCQRFAIGASPRAARERLGWDQDRR
                     HILAVRRLARRMGLPELISSMVEVRRKLPEARLLIAGRGDQEGALRRLIQELDLADSV
                     QLLGFVPDDLLPWAYRAVDLTVVPSTELEGFGLITAESLAAGTPVLVSPVGGLPEAVR
                     PLAPELVLEGCAPAHLASGIIGALSGTRPLPTSEQCRSYARERFDWPVIAAQTREAYS
                     SVLG"
     gene            4161..5507
                     /locus_tag="VGP50_17035"
     CDS             4161..5507
                     /locus_tag="VGP50_17035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV8029141.1"
                     /translation="MSSAEIEGPRQAADQPGLRPRGGQPRGTAKASFEVVIVCDHAHI
                     SGGLAQVAVASARGLRRRGHRVIFFAAVPPVDPSLLEAGVEVVCLGQPDMLHDPSRLK
                     AAGRALWNAGAARQLADLLASLDRHRAIVHLHGWSKALSPSILRASRRSGAASVHTLH
                     DYVAVCPNGALFNYVKRENCTLRPMSAACLVSNCDARHYGHKLWRVGRQLALTSLGGA
                     LAGEDVIYLSKAQRGIVAPLLPADTRLHYLPNPIDLEDRGPADVTANDTFVFIGRLSQ
                     EKGAAVFAEAARRAGVKALFVGDGPMRQAILSAAPGAAITGWVDNAQVRAHLRRARAL
                     VFPSLWYETFGLSVHEALANGVPPIVSDNTTSAEALEHEVTGLLFRSGNVDDLTAQIR
                     RLSDGDAAGRIGRAAYDRYWREPLTLDRHVDGLEELYGLLLCERAGKGKDHGRGYH"
BASE COUNT          854 a         1896 c         1910 g         1005 t
ORIGIN      
        1 gcggaccgtg atgttcgcat cggcgctgcc gggcgaaggg aagagcgtgt cgtcgatggc
       61 gctggcgctg ctgatggcga atatcgggca gcgggcgatc atcgtcgatt gcgatacgcg
      121 ccggccctcc gtgcacacca ctttcggtct ctcccgctcc gacggcctca ccgaatatct
      181 cgagggggcg ccactcggct gcgtggtccg caccgtcggg agcccggcgc tgagcgtgat
      241 cacggccggg catcccgtct cgcatcccgc cgctcttttt gcttcggccc ggatgaagca
      301 gctcctcgcc gatctgacga attcctacga cctcgtagtc gtcgacagct gcccggtatt
      361 gacctctgcc gatgcgctgg tgctggcgcc gctcgtcgat aagacggttt ttctcgtcca
      421 atggggaagg accccgcaga aggctgccga gcgtgccctg gagcttctca gagaggcggg
      481 cgcggatgtg gccggcacga tgctgtcgat ggtcaacatc caaaagatga tgacgcatga
      541 tttgggcgcc ggctcctatc ggaaggtgcg gcgctattac cagacgacgt aaggaccgcc
      601 ggtctgctgc gaagcccggt gccggccgat ttgacgcacg cgcgaaccag agtcgaggaa
      661 ttgtcatgaa ggtcgcgatt atacactact ggctcgtggg gatgcgcggc ggcgagaaag
      721 tggtcgaggc cttgtgcgac atgtttccga cggccgacgt ctatacgcac gtctatcgtc
      781 cggagcggat atccgagtcg attcgtcgtc acagcgtcaa gacgacattc atcgcgcgtc
      841 tgccgttttc gcgccggcac tatcaaaaat atctgccgct gatgccgctg gcgctggagc
      901 agctcgacct gcaggactac gacctcgtga tcagcagcga gtccggaccg gccaagggcg
      961 tcatcacgcg tccggatgcc ctgcatgtct gctactgcca caccccgatg cgctacgtgt
     1021 ggagcggcta tcactcctat cgcggccggt ccaatccgct gaccaaggca gtgatgccgg
     1081 cgctcatgca ccggttgcgg atgtgggatc ttgccagcgc ggcgcgggtc gaccacttca
     1141 tcgccaattc ccacaatgtc gccgagcgca tcaagaaata ctatcgccgc gacgccagcg
     1201 tcatctatcc gcccgtggag atgcaggggg tcgacctgca ccggcgcacc gaggatttct
     1261 acctcttcgt cggccaactc gtcccctaca agggcgccga cgtcgccatc gccgccttca
     1321 atcgcatggg caagcgtctc gtcgtcatcg ggcacggcga agattatcag cgtctgagga
     1381 aaatgagcgg tccgacggtc gagctggtcg gctggcagga cgcggccacg ctgcaagact
     1441 actatgcgcg ctgcaaagcg ctcgttttcg tcgccgacga ggatttcggc atggtgcccg
     1501 tcgaagcgat gagcgccggg cggccggtga tcgcctatgg ccgcggcggc gcgctcgaga
     1561 ccgtgctgcc cggcaagacc ggcgtgctct tcgagagcca gacggtcgac tcgttgatat
     1621 cggcggtcga tcatttcgaa tcggtcgccg acacgttcga ccgcacgcgg attgcggcgc
     1681 acgccgcggc attttcgaag acggttttca acgcccagat ggccgagacc ctgggccgat
     1741 ggctcgacga gcacggacgc gcccagaggc gcgcgctgcc gccgttggag agcgttgccg
     1801 acaaggtcgc ggcggcggcg gcttagcaga ggcgggcgat aacaccgatg cgggttgcca
     1861 tcgtcacgcg gtacctgaag aagcaggacg ggcagggccg cgtcaacttc gaattggcgc
     1921 aggagcttct gtgccgcggc cattcggtac tcctcgtggc ctcggaagtc gagccgagcc
     1981 tgctcgaaat ccgcggcgcg gagtgggcga ggatcccggt gccgcccgtt cccacctatc
     2041 ttttgcgcga gcagatattc gccgtgctgt cgagcgccgt gttggcgcgc gagcaccggc
     2101 gactccaggc gatcgtggtc aacggttttg ccacgtggtg gcgcagcgac gtgaatatcg
     2161 tcaatttcgt gcatcacgcc tggctcaagt cgccgttgca cccggcgcgc ggcaggctgg
     2221 gcctcgccgc gctctatcgc tggttctaca cagcggtcaa tgcgaaggcc gagcgcgcgg
     2281 ctttcgccca ggcacggtcg gtggtcgcga tatcggagat cgtgcgccgc caggtcgtcg
     2341 actgtggcgt cgatgcggac cggatcacgg tgatccccgg cggtgtcgat ctcctcgcct
     2401 tttcgccggg caccgggcgg cgctccgact tcggccttcc cgaagacctt ttcatcggct
     2461 tgttcgccgg cgacctgcgc acgacgcgca agaacctcga cagcgttctg aaggcgatga
     2521 cgcgggtacc ggacatgcat ctcgccgtcg caggcgacac cgcgaaaagc ccctatccgg
     2581 caatggcgcg gtccttgggc attgccggcc gcgttcactt cgtcggcatg agcactgagc
     2641 tcggggcgct gatgcgcgcc gtggacgcct ttgtcttccc cacgcgattc gagccttttg
     2701 ggctcgtcct cctcgaagcc agcgcctgcg ggctgccgat cgtgacctcg cgcgcagcgg
     2761 gcgcctgtga aagcctcgac ggcgacggca tgatcgtgct cgacgacccc gacgatgtcg
     2821 acggcctcgc cgccgccctg gcaatgctgg cgagcgacac ggcactccgg cgacggatgg
     2881 gcaggtcggc ccgcgtcgcc gccgagcgcc tttcctggaa gagcgcggtg gcgcgacagg
     2941 cgcgcctgat cgaagccgtc gcccgcgtct gaggagaacc ggcgtgacca cgctcgccgc
     3001 gaccgtgccc gctccgatca ccgcggggaa gcgcgccttg tcgcttcttc agatcggcgc
     3061 cggctggttc ccggaattct gtgccggcgg ggaaaacgtc ttttatcatt tggcgaagca
     3121 cctgcccgat gccggggtcg ccgtccgcgg gcttgtcgtc ggctccgcca acgtcttcgc
     3181 gcagtcggct ggacgcgtcc ttggctttgc gccgcgctcc gctccgctgg ccacccggtt
     3241 gtggcgggcg cgccaggcga tcgttgcgag cttcgccgcc ggcgcccccg atctggtggc
     3301 cacacatctt gctcttttcg ctttgccggc cctcgacaag ctgaggtcac ggcctttgat
     3361 cgtgcatttt cacggcccct gggcggccga aagcgcgctc gaaggggccg gctggatgtc
     3421 ggtcggaagc aagcgcgcca tcgagcgtct cgtctacaat cgtgcggatc gcgtgatcgt
     3481 gctgtcgcgg gctttcggcg aggtcctgcg gcgggcctac ggcgtcgacg cggcccgcat
     3541 cacggtcatt cccggcggcg tcgattgcca gcgcttcgcc atcggcgcat cgccccgcgc
     3601 cgcgcgcgag cggctgggct gggaccagga ccggcggcat atcctggcgg tccggcggct
     3661 ggcgcgccga atgggcctgc ccgagctgat ctcgagcatg gtcgaagtgc gacgcaagct
     3721 gccggaggca cggcttctca ttgccgggcg cggcgatcag gagggcgcgc tgcggcggct
     3781 catccaggaa ctcgatctgg ccgacagcgt ccagctgctc ggctttgtcc ccgacgactt
     3841 gctgccctgg gcttatcgcg ccgtcgacct cacggtggtt ccctcgaccg agctcgaggg
     3901 cttcgggctg attacggcgg agtcgcttgc ggcggggacg ccggtcctcg tctcgccggt
     3961 cggcggactt cccgaggccg tccgcccgct cgcgccggaa ttggtgctcg agggatgcgc
     4021 gccggcgcat ctggcaagcg gcatcatcgg cgcgctcagc gggacgcgcc cgctccccac
     4081 gagcgagcaa tgccggagct atgcccgcga gcgcttcgat tggcccgtca ttgccgccca
     4141 gacacgggag gcctattcca gtgtcctcgg ctgagatcga aggaccgcgg caggccgccg
     4201 accagccggg cctgcgcccg cggggcggcc aaccccgcgg caccgccaag gcgtccttcg
     4261 aggtcgtgat cgtctgcgat cacgcccata tctcgggcgg ccttgcgcaa gtggcggtgg
     4321 cgagtgcgcg ggggctgcgc cgtcgcgggc accgcgtcat cttttttgcg gccgtgccgc
     4381 cggtcgatcc gtcgctgctc gaagcaggag tcgaggtcgt ctgcctcggc cagccggaca
     4441 tgctccatga tccttcgcgc ctcaaagcgg ccggtcgggc attgtggaat gctggcgcag
     4501 cgcggcagct cgccgatctc ctcgccagcc tcgatcgcca tcgggcgatc gtgcaccttc
     4561 acggctggag caaggcgctg tcgccgagca tcttgagagc atcccgccga tccggcgcag
     4621 cgtcggtgca tacgcttcat gactatgtcg cggtatgccc gaacggggcg ctgttcaatt
     4681 atgtcaagcg cgagaactgt acgctgcggc cgatgtcggc ggcctgcctt gtctccaatt
     4741 gcgacgcccg ccactacggc cacaagctct ggcgcgtcgg ccgccaattg gcgctgacgt
     4801 ccctgggcgg agccctggcc ggcgaggacg tgatctatct cagcaaggcc cagcgcggga
     4861 tcgtggcgcc gctgctgccc gccgacacga ggctccacta ccttcccaac ccgatcgacc
     4921 tcgaagaccg cggccccgcg gacgtcaccg ccaacgacac cttcgtgttc atcggacgcc
     4981 tgtcgcagga gaagggcgcg gcggttttcg cggaagccgc gcggcgcgcc ggcgtcaagg
     5041 cccttttcgt cggcgacggc ccgatgcgcc aggcgattct gagtgcggcg ccgggtgcgg
     5101 cgattaccgg ctgggtcgac aacgcgcagg tcagggccca tctccggcgc gcaagagccc
     5161 tggtgtttcc gtcgctctgg tacgagactt tcggcctcag cgtccacgag gccctcgcga
     5221 acggggttcc gccgatcgtc agcgacaaca ccacgagcgc cgaggcattg gagcacgaag
     5281 tgacgggact gctgttccgc agcggcaacg tcgacgacct gacggcccag atccgcaggc
     5341 tgagcgacgg cgatgccgcg ggacggatcg gccgggcagc ctatgatcgc tactggcgcg
     5401 agcccctgac gctcgatcgc cacgtcgacg ggctcgagga actctacggg cttttgctct
     5461 gcgagcgggc aggaaaaggg aaggaccatg gcagaggcta tcattgagca gggcggtccg
     5521 ccgtcgaacg gcatgagcct tcgggacttg atcgtgcggc ggtggctccc gttcgtgctc
     5581 tcccgtccgc aggcccagat cgtcaatcga ttgctcctgg gtatcgcgct tcgcggcatg
     5641 ggcgtcgaca atcacgatct gacgc
//
LOCUS       DASYNS010000556         5618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1232462, whole genome shotgun sequence.
ACCESSION   DASYNS010000556 DASYNS010000000
VERSION     DASYNS010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232462"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VGP50_17040"
     CDS             complement(<1..455)
                     /locus_tag="VGP50_17040"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEV8029142.1"
                     /translation="MPLRLDTGTPGFAADFSRLVTAKREVEEDVDAAVAEILAAVRAG
                     GDAAVIDYTRRFDRLELTPATMRVAPAAIAAAAKSCAGDTLAALRLAAERIEAYHRRQ
                     LPQDLDYVDAAGVRLGARWRPIAAVGLYVPGGTAAYPSSVLMNAIPAKVA"
     gene            complement(445..1107)
                     /gene="hisG"
                     /locus_tag="VGP50_17045"
     CDS             complement(445..1107)
                     /gene="hisG"
                     /locus_tag="VGP50_17045"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEV8029143.1"
                     /translation="MQVNEKLVLALPKGRILSEVLPLVRRAGIEIEHAFDDPDARQLR
                     FATNRPELDVIRVRNFDVATFVAFGAAHIGVAGNDVLMEFEYPEIYAPLDLKVGHCRL
                     VVAAPAEDLGRDDPTRWSQLRIATKYPEITRRHFAARGVQAECIKLNGAMELAPSIGL
                     SRQIVDLVQTGSTLRANGLVEIERIADITSRFIVNRVALKTRAEEVTGWIERFREAAD
                     AA"
     gene            complement(1334..1765)
                     /locus_tag="VGP50_17050"
     CDS             complement(1334..1765)
                     /locus_tag="VGP50_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014248466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEV8029144.1"
                     /translation="MAQQALKLRGEDEEDLSVISAILQDALVAVGEMAYFPDEGRFIL
                     VANRFRWEPQPGGDQRLERQLAGLRIHGVRGVQRRGFNPRDGERLLVLLAIRAEDSVV
                     YLDFAGGSRVRLETDGILFYLDDLGEPWPTRWRPKHPVEEG"
     gene            complement(1774..3060)
                     /gene="murA"
                     /locus_tag="VGP50_17055"
     CDS             complement(1774..3060)
                     /gene="murA"
                     /locus_tag="VGP50_17055"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV8029145.1"
                     /translation="MDRIRIRGGRPLRGKIPIGGAKNAALPLMTASLLTEDTLVLANL
                     PLLADITTLSHLLVQHGVSVTMAGAEGPEEHGHVLEFRAKRIVSTTAPYDLVRKMRAS
                     VLVLGPLVARCGEARVSLPGGCSIGTRPVDLHLKGLERLGASVELRQGYIHASAPKGL
                     QGAEIIFPIVSVGATENLLMAATLAEGETSLVNAAREPEIVDLARCLSAMGAVIEGAG
                     TDRIRVQGVKRLHGARHAVIPDRIETGTYMMAAAVTGGAVELLGAVLEPVGAVARILE
                     GAGVTVSETPHGLAVARRNGRLTGVDVMTEPFPGFPTDLQAQMMAVMATAEGAAMITE
                     TIFENRFMHVPELCRMGANINVHGASAMVRGVPRLTGAPVMATDLRASSSLVLAGLAA
                     EGETVINRVYHLDRGYERLEEKLAACGADIERLVAD"
     gene            complement(3124..3678)
                     /gene="dcd"
                     /locus_tag="VGP50_17060"
     CDS             complement(3124..3678)
                     /gene="dcd"
                     /locus_tag="VGP50_17060"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEV8029146.1"
                     /translation="MPIMPDSWIRETALKHGMIEPFVEKQTRQGVISYGLSSYGYDAR
                     VSREFKIFTNVDSAIVDPKAFSPHSFVDRETDICVVPPNSFALATTVEYFRIPRDILV
                     VCLGKSTYARCGIIVNVTPLEPEWEGHVTIEISNTTPLPARIYALEGICQFLFFKGDK
                     PCEVSYADRAGKYMRQRGVTLPKL"
     gene            complement(3752..3931)
                     /locus_tag="VGP50_17065"
     CDS             complement(3752..3931)
                     /locus_tag="VGP50_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006116944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029147.1"
                     /translation="MSDTPKERQSPKAGRTAASGGRQARLAAALRANLARRKAQARQR
                     APAAARPVTTPEERG"
     gene            complement(4066..4689)
                     /locus_tag="VGP50_17070"
     CDS             complement(4066..4689)
                     /locus_tag="VGP50_17070"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEV8029148.1"
                     /translation="MKSLIVSTAVAALLAAAPALAQSTNPPNMNPDSTTKSGSAAATG
                     AAIEGSSDARFIDHVARDNEGEIELGKLAVQKAQSPEVKALAQRLVTDHSKANQQLKR
                     LAQKESVSVPTGAGKEQKDLRAQLEKLNGAAFDRAFVDAEVKDHQNDITFYQNESNRL
                     QDPQLRSFAQQTLPVLQEHLQMAERVEVGASSGSSTMTAPSMLPSQR"
     gene            complement(4838..5041)
                     /locus_tag="VGP50_17075"
     CDS             complement(4838..5041)
                     /locus_tag="VGP50_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029149.1"
                     /translation="MSDNDEAVPEVLAAFQAARQAGRAAVDCYRAGVAAWRRHHPDQA
                     PEYAAKRAVAVILAHNIELKVAD"
     gene            complement(5157..5393)
                     /locus_tag="VGP50_17080"
     CDS             complement(5157..5393)
                     /locus_tag="VGP50_17080"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HEV8029150.1"
                     /translation="MTPERYAEIAKRAYALWELEGQPAGRDVDHWLAAEAELEATWHV
                     EPQVESAREDTPAPPAAAAKRSSVRQRRKASASS"
     gene            complement(5408..>5618)
                     /locus_tag="VGP50_17085"
     CDS             complement(5408..>5618)
                     /locus_tag="VGP50_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029151.1"
                     /translation="DLLFATERAIALYRQPLAWRKVQRQAMAQNFDWSESARRYLALY
                     RDLVPEPALISDPIVAEPMRQEKAG"
BASE COUNT          877 a         1876 c         1938 g          927 t
ORIGIN      
        1 gcgaccttgg cgggaatggc gttcatcagc accgaggacg gataggccgc ggtgccgccg
       61 ggcacataaa gtccgaccgc cgcgatcggc cgccagcgcg cgccgagccg cacgccggcc
      121 gcgtcgacat agtcgagatc ctgaggcagc tgccggcggt gataggcctc gatgcgctcg
      181 gcggcgaggc ggagcgcggc caaggtgtcg ccggcgcagg atttcgccgc ggcggcgatc
      241 gcggcgggcg caacccgcat cgtcgcgggc gtgagctcga ggcgatcgaa gcgccgcgtg
      301 tagtcgatga ccgcggcatc gccccctgcg cggacggccg ccaggatctc ggccacggcc
      361 gcgtcgacat cctcttcgac ctcccgcttg gcggtgacca ggcgggagaa atcggccgcg
      421 aagcccggcg tgccggtgtc gagcctaagc ggcatcggcc gcctcgcgga aacgctcgat
      481 ccagccggta acttcctcgg cgcgggtctt gagggcgacg cggttgacga tgaagcgcga
      541 ggtgatgtcg gcgatccgct cgatctcgac gaggccgttg gcgcgcagcg tcgatcccgt
      601 ctggacgaga tcgacgatct ggcggctcaa gccgatgctg ggcgcgagct ccatcgcgcc
      661 gttgagcttg atgcactccg cctggacgcc gcgcgccgcg aaatgccggc gcgtgatctc
      721 cgggtatttg gtggcgatgc gcagctggct ccagcgcgtc gggtcgtcgc ggccgagatc
      781 ctcggcaggc gccgcgacga cgagacggca atgcccgacc ttcaagtcga gcggcgcgta
      841 gatctcgggg tattcgaact ccatcagcac gtcgttgccg gcgacgccga tatgcgccgc
      901 gccgaaagcg acgaaggtcg cgacgtcgaa attgcgcacg cggatgacgt cgagctccgg
      961 ccggttggtg gcaaaacgca gctggcgggc gtcggggtcg tcgaaggcgt gctcgatctc
     1021 gatcccggcg cggcgcacca gcggcagaac ctcgctcagg atccgcccct tgggcagcgc
     1081 cagcaccagc ttctcattga cctgcaagaa ccgcctccta aacggaccac gcggggccaa
     1141 tgtactgaag acagagaggc ttcacatccc ctctcccctt gtgggagagg gaggggcccg
     1201 ttgcgcagca acgggagggt gaggggtggg gcttaatggc tccggtcccc agcgatctcg
     1261 gcgagagccc ctcaccctcc cgacgcttcg cgtcgggccc ctccctctcc cacaagggga
     1321 gcgggggtgt tcgtcacccc tcttcgacgg ggtgcttggg gcgccagcgc gtcggccagg
     1381 gctcgcccag atcgtccaaa tagaaaagga tgccgtcggt ctcgaggcgg accctgctgc
     1441 cgccggcgaa atcgagatag accacgctgt cctcggcgcg gatcgcgagc agcacgagga
     1501 gacgctcgcc gtcgcgcggg ttgaagccgc gccgctgaac gccgcggacg ccatggatgc
     1561 gcaagcccgc gagctgtcgc tcgagccgct gatcgccgcc cggctgcggc tcccagcgga
     1621 agcggttggc caccaggatg aagcggccct cgtccgggaa ataggccatc tcgcccactg
     1681 ccaccagcgc atcctgcaga atggcggaga tgaccgaaag gtcttcctcg tcctcgcccc
     1741 tgagcttgag cgcctgctgg gccatcctcc cgctcagtcc gcgaccaggc gctcgatgtc
     1801 ggcgccgcaa gccgcaagct tctcctcgag gcgctcgtag ccgcgatcga gatggtagac
     1861 ccggttgatg acggtctcgc cctctgccgc aagccccgcc agcaccagcg aagacgaggc
     1921 ccgcagatcg gtcgccatga ccggcgcgcc ggtgagcctg ggcacgcccc gcaccatggc
     1981 ggaggcgccg tgcacgttga tgttagcgcc catgcggcaa agctcgggca catgcatgaa
     2041 gcggttctcg aagatcgtct cggtgatcat cgccgcgcct tcggccgtgg ccatcaccgc
     2101 catcatctgc gcctggaggt cggtcgggaa gcccggaaac ggctcggtca tgacatcgac
     2161 gccggtgagc cggccgttgc gccgggcgac ggcgaggccg tgcggcgtct cgctcaccgt
     2221 gacacccgcg ccttcgagga tccgcgcgac cgcgccgacc ggctcgagga cggcgcccag
     2281 gagctcgacg gcgccgccgg tcacggccgc ggccatcata taggtgccgg tctcgatgcg
     2341 gtcggggatg acggcatggc gcgccccgtg cagccgcttc accccttgaa cacggatgcg
     2401 gtcggtgccg gcgccttcga tgacggcgcc cattgccgag aggcagcgcg cgagatcgac
     2461 gatctcgggc tcgcgcgccg cgttgacgag cgaggtctcg ccctcggcga gggtcgcggc
     2521 catcagcaga ttctcggtcg ctccgaccga cacgatgggg aagatgatct cggcgccctg
     2581 gaggcccttg ggcgcgctcg catggatata gccctggcgc agctcgacgg aggcgccgag
     2641 ccgctcaagc cctttcagat gcaggtcaac gggccgggtg ccgatggagc agccgcccgg
     2701 cagcgacacg cgggcctcgc cgcaacgcgc gaccagcggt cccagcacca gcaccgacgc
     2761 ccgcatcttg cggacgagat cgtagggcgc cgtggtcgag acgatccgct tcgcccggaa
     2821 ctccagcaca tggccgtgct cctcggggcc ttcggccccc gccatcgtca ccgagacgcc
     2881 atgctgcacc agaaggtgcg agagcgtggt gatgtcggcg agaagcggca gattggcgag
     2941 gacgagcgtg tcctcggtga ggaggctcgc cgtcatcagc ggcaaggccg cgttcttggc
     3001 gccgccgatg ggaatctttc cccggagggg gcggccgccg cggatgcgga tacgatccat
     3061 gtgcttttgt cctgctgtcg atcccggtgg ggatcttcta gcgcgacttt gtggcgaaat
     3121 tcgtcagagc ttcggcaggg tcaccccgcg ctgacgcatg tatttgccgg cacggtcggc
     3181 gtaggagacc tcgcagggct tatcgccctt gaagaaaaga aactggcaaa tcccttccaa
     3241 ggcgtaaatg cgcgccggca aaggcgtggt attcgagatc tcgatggtga cgtgcccttc
     3301 ccattcgggc tcgagcggcg tcacgttgac gatgatgccg caacgggcgt aggtcgactt
     3361 gcccaggcac accaccagga tgtcgcgcgg gatgcggaaa tattcgaccg tcgtcgccag
     3421 cgcgaagctg ttgggcggga cgacgcagat gtcggtctcg cggtcgacga agctgtgcgg
     3481 cgagaaggcc ttgggatcga cgatggcgct gtcgacattg gtgaagatct tgaactcgcg
     3541 gctgacccgc gcgtcgtagc cataagaaga gaggccgtaa gagatcacgc cttgccgcgt
     3601 ctgcttctcg acgaagggct cgatcatgcc gtgcttcagc gccgtctcgc ggatccagct
     3661 gtcgggcatg atgggcatgt gcagcgcttt cgttgagcca gggcacgccc ttctagagga
     3721 tgcgccgcga cggctcaagc cgaggcggcg atcagccgcg ctcttcgggc gtcgtcaccg
     3781 gacgagcggc agcaggcgcc cgctgccgcg cctgcgcctt gcggcgggcc aggttcgcgc
     3841 ggagcgctgc ggcgagtcgc gcctgccgac cgccgctcgc ggcggtgcgg ccggctttgg
     3901 gagattgtcg ctctttgggc gtgtcgctca tcgtctcgcc gcaagagagt ggatcgcggc
     3961 gcagcgcccc gtcaagcggt ggtcatcccc ggaaaaagga gcggagcgca cagccgctct
     4021 gtgcgctcca ccgggccctc gacgacgctt cgcgtcgcga gtcgctcagc gctgcgaagg
     4081 cagcatgctc ggggccgtca tggtcgagct gcccgaggag gcaccgacct ccacccgctc
     4141 ggccatctgc agatgctcct gcaacaccgg aagggtctgc tgcgcgaagg atctgagctg
     4201 cggatcctgg agtcgattgc tctcgttctg atagaacgtg atgtcgttct ggtgatcctt
     4261 cacctcggcg tcgacgaaag cgcgatcgaa agccgcgccg ttgagctttt ccagctgcgc
     4321 ccgcaggtcc ttctgctcct tgccggcgcc ggtgggcacc gacacgcttt ccttctgcgc
     4381 gagccgtttc aattgctggt tcgccttgct gtggtcggtg acgagacgct gcgccaaggc
     4441 cttgacctcg ggactctggg ccttctgcac ggcgagcttg ccgagctcga tctcgccctc
     4501 attgtcgcgt gccacgtgat cgatgaagcg tgcgtcggag ctgccctcga tcgccgcgcc
     4561 cgtcgccgcg gcgctgccgg acttcgtggt cgagtccgga ttcatgttcg ggggattcgt
     4621 ggattgcgcc agcgcgggcg cagcggcgag gagagcggcc acggcagtgg aaacgatgag
     4681 tgacttcatg gcggtcgtgc ctccagacgg tttccctttg ctagggaacg ggggtcctcg
     4741 gccgacgttc cgtggcggcg ttgtggcaag cgccgcgcga tgaaactgat cctcacaaag
     4801 aagtcacaag gcgatggccc gattcgctcg agctcgctta atccgcgacc ttgagctcga
     4861 tgttatgcgc gagaatcacc gcgacggcgc gcttggccgc atattcgggc gcctgatcgg
     4921 gatgatggcg ccgccacgcc gccacgccgg cgcggtagca gtcgacggcc gcgcggccgg
     4981 cctggcgcgc cgcctggaac gctgccagca cttccggaac cgcctcgtcg ttgtcgctca
     5041 ttgttgcacg cagttgcctt ctcccgttgg agctaacagg aggggcgcag aaatattccc
     5101 tcgagcaccg atgccacagc gacggcgcga aatcgcgcga cgggaactcg tgcttgctaa
     5161 gacgaggcgg atgctttgcg ccgctgccgg acgctgctgc gtttggccgc ggcggctggc
     5221 ggcgccggtg tgtcctcccg cgcggattct acctgcggtt ccacgtgcca tgtcgcctcg
     5281 agctcggctt cggcagcgag ccaatgatcg acgtctcggc cggccggctg tccttccagc
     5341 tcccacagcg cataggcgcg cttggcgatc tcggcatacc gctcgggggt catccaggcc
     5401 tcctctccta tcctgccttt tcctggcgca tcggctcggc gacgatcgga tcggatatca
     5461 acgcgggctc cggcacgaga tcccgataga gcgccaagta gcggcgcgcc gattccgacc
     5521 aatcgaaatt ctgggccatc gcctgccgct gcaccttgcg ccaggcgagc ggctgccggt
     5581 agagcgcgat cgcccgctcg gtcgcgaaca gcaggtcg
//
LOCUS       DASYNS010000557         2848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2795968, whole genome shotgun sequence.
ACCESSION   DASYNS010000557 DASYNS010000000
VERSION     DASYNS010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2795968"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="VGP50_17090"
     CDS             <1..1397
                     /locus_tag="VGP50_17090"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HEV8029152.1"
                     /translation="LTPTLSPGGGEGEVIVARGRAMSHGSILGLSPAWVATGTFALSY
                     LVIITERLNRSITALLGASAMVVTGVLTQEEAIRGIDFNTIGLLTGMMVLVSIARRSG
                     MFEYLAVWSAKQSRAEPWIMLAMLSLVTAVVSAFLNNVAMVLLMAPVTLAVARQLEVP
                     PYPFLFAEIFASNIGGTATLIGDPPNIMIGSAASLGFDDFIVNLTPVIVLVMAVQLLA
                     THLIWGRTMRTTPEARARVMALDPGKMIADRPLLRASLAVMGGEILAFVFARQLALEA
                     GTIAIAGAAVLMLCHNIEHHRESFRQTEKLTEAFAEVDWITIFFFLGLFVIVRGIDTT
                     GVLDVLAQRLIAATGHDLGTAAAAVLWGSAVLSAIVDNIPFVAAMIPLIKALGPELGG
                     APHILPLWWALSLGACLGGNGTLIGAAANLSVAGIAERNGVSFSFLTFTKYAFGLMLL
                     SVAISHVYLRLRYF"
     gene            1627..2247
                     /gene="eda"
                     /locus_tag="VGP50_17095"
     CDS             1627..2247
                     /gene="eda"
                     /locus_tag="VGP50_17095"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV8029153.1"
                     /translation="MHEMFRDVAVIPVLTIARAADAVPLAEALLRGGLRVIEVTLRTP
                     AAAEAIAEIRRRVPDVVVGAGTLLKGADIGRAVAAGARFLVSPGLTPELAAAGLASER
                     PYLPGVVTPSEVMAARDLGLSLLKFFPATGAGGVDTLATYAPVFPGVAFCATGGVDRA
                     NAGAYLALANVTMVGGDWMAPADAVAAGDWRKIEALAREAAALRRK"
     gene            2275..>2848
                     /locus_tag="VGP50_17100"
     CDS             2275..>2848
                     /locus_tag="VGP50_17100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8029154.1"
                     /translation="MAQTLRVNGYDMSYVERGEGVPLLLIHGTLCDYRYWTAQMEPLG
                     HGRRVIAVSLRHCYPERWDGAGADFTITQHGADVGAFIAALDAGPVHLLGHSRGGRVA
                     FDVARNFPDRVSSLILADPGGELDESLRPRGEAPPARTALWECGQLVKQGEIDRGLEK
                     FIELISRPGIWSAMPEPAKNMLRDNARTLIG"
BASE COUNT          400 a         1000 c          954 g          494 t
ORIGIN      
        1 ccctcacccc gaccctctcc cccggtgggg gagagggaga agtaatcgtt gcgcggggcc
       61 gggcgatgag ccacggctcg atcctcgggc tctcgccggc atgggtggcg acgggcacct
      121 tcgcgctgag ctatctcgtc atcatcacgg agcggctcaa ccgctcgatc accgcccttc
      181 tcggggcaag cgccatggtc gtgaccggcg tcctcaccca ggaagaggcg atccgcggca
      241 tcgatttcaa cacgatcggg ctcctcaccg gcatgatggt gctggtgtcg atcgcgcggc
      301 gcagcggcat gttcgaatac ctggcggtgt ggtcagccaa gcagagtcgc gccgagccct
      361 ggatcatgct ggcgatgctg tcgctggtca cggcggtcgt ctcggccttt ctcaacaacg
      421 tcgcgatggt cctgctgatg gcgccggtga cgctcgccgt cgcgcgccag ctcgaggtgc
      481 cgccctatcc ctttctcttc gccgagatct tcgcctcgaa catcggcggc acggcgacgc
      541 tgatcggcga tccgcccaac atcatgatcg gctccgcggc cagtctcggc ttcgacgatt
      601 tcatcgtcaa cctgacgccg gtgatcgtcc tggtgatggc ggtgcagctc ctggcgacgc
      661 atctgatctg gggccggacg atgcgcacca cgcccgaggc gcgcgcccgc gtgatggcgc
      721 tcgatcccgg caagatgatc gccgaccggc cgctgctccg cgcgtcgctc gccgtcatgg
      781 ggggcgagat cctggccttc gtcttcgccc gacaactcgc gctcgaggcc ggcaccatcg
      841 ccatcgccgg cgccgccgtg ctcatgctct gccacaacat cgagcatcac cgcgagagct
      901 tccggcagac ggagaagctg accgaggcct tcgccgaggt cgactggatc acgatcttct
      961 tctttctcgg gctcttcgtc atcgtgcgcg gtatcgacac gacgggggtg ctcgacgtcc
     1021 tggcgcaacg tctcatcgcc gccacgggcc atgacctcgg gaccgccgcc gctgccgtcc
     1081 tctggggatc ggcggtgctg tcggcgatcg tcgacaacat ccccttcgtc gccgcgatga
     1141 tcccgctcat caaggcgctc ggccccgagc tcggcggcgc gccgcacatc ctgccgctgt
     1201 ggtgggcgct ctcgctcggc gcctgcctcg gcggcaacgg cacgctcatc ggcgccgccg
     1261 ccaatctgag cgtcgccggc atcgccgagc gcaacggcgt ctccttcagc ttcctcacct
     1321 tcaccaaata cgccttcggc ctgatgctgc tcagcgtggc gatcagccat gtctatctgc
     1381 gcctgcgcta cttctgacgg ggaggagaag aaaaaagagt caccacagag gcagtgaggc
     1441 agtgaggcag tgaggagacg aaatcggcgc gcaaagcgcg cctttttttc ttctcactgc
     1501 ctcactgcct ctgtggtgaa tcttttcgtc gctgcgcaac gccgtccatg cctacgtggt
     1561 caatcttctt gttgcacgcc tcgggtcggc ggcctatgtc tgcgcgcgag cgagcagggg
     1621 gccctgatgc acgagatgtt ccgcgatgtg gccgtgatcc cggtcctcac catcgcgcgc
     1681 gcggcggacg cggtgccgct cgccgaggcg ctgctgcgcg gcggcttgcg cgtcatcgag
     1741 gtcaccttgc gcacgccggc ggcggcggag gcgatcgccg agatccggcg gcgcgtcccc
     1801 gatgtcgtcg tcggcgccgg cacgctgctc aagggcgccg atatcggacg cgccgtcgcc
     1861 gcgggggcgc gctttctggt gagcccgggc ctcacgccgg agctcgcggc cgccgggctc
     1921 gccagcgagc gcccctatct gccgggcgtg gtcacgccgt ccgaggtcat ggccgcgcgc
     1981 gatctcgggc tctcgctgct caaattcttt ccggcgacgg gggcgggcgg cgtcgacacg
     2041 ctcgccactt acgcgccggt ctttccgggc gtcgccttct gcgcgacggg cggcgtcgac
     2101 cgggcgaatg cgggcgccta tctcgctctc gccaatgtga cgatggtcgg cggcgactgg
     2161 atggcgcccg ccgacgccgt cgcggccggc gactggcgga agatcgaggc gctggcgcgg
     2221 gaggccgcgg cgctccggcg caagtgacgt atttctagcg aaccgagagg gatcatggca
     2281 cagacgctgc gcgtgaacgg ttacgacatg agctacgtcg agcgcggcga gggcgtcccg
     2341 ctgctgctca ttcatggcac gctttgcgac taccgctatt ggacggcgca aatggagccg
     2401 ctcgggcacg gccggcgcgt catcgccgtg agcctcaggc attgctaccc cgagcgctgg
     2461 gacggcgccg gcgccgactt cacgatcacg cagcatgggg ccgatgtcgg cgcgttcatt
     2521 gccgcgctcg acgcggggcc cgtgcatctg ctgggccatt cccgcggcgg ccgcgtggcc
     2581 ttcgacgtcg cgcggaattt ccccgaccgc gtctcctcgc tgatcctcgc cgatccgggc
     2641 ggcgagctcg acgagagcct gcggccgcgc ggcgaagcac ccccggcgag aaccgcgctt
     2701 tgggaatgcg ggcagctcgt caagcagggc gagatcgacc gcggcctcga gaaattcatc
     2761 gagctgatca gccgtcccgg catctggagc gcgatgcccg agccggccaa gaacatgctc
     2821 cgcgacaatg cccgcacttt gatcggcc
//
LOCUS       DASYNS010000558         6018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4044266, whole genome shotgun sequence.
ACCESSION   DASYNS010000558 DASYNS010000000
VERSION     DASYNS010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4044266"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..2865)
                     /locus_tag="VGP50_17105"
     CDS             complement(76..2865)
                     /locus_tag="VGP50_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEV8029155.1"
                     /translation="MAKRMLIDAAHPEETRVVVLNNNRLEEFDFETSTKKQIKGNIYL
                     AKVTRVEPSLQAAFVEYGGNRHGFLAFNEIHPDYYKIPVSDRMPYHDRAPPSRAEPGE
                     EGAEELPGGDAAPRPTEGADEPEGAEADEVMKIVNAAEIEAERAAPAEAHDPDIVYER
                     RDAAEEPGGEIGTESTSETEAEVAAAGGDGDAGDEPNGEAERHEPHEINGNGANGGER
                     APAEHENGGEETRPDVIETVGGDEMEEAQRQRARHYRHYKIQEVIKRRQIMLVQVTKE
                     ERGNKGAALTTFLSLAGRYCVLMPNTPRGGGVSRKITSVTDRRRLKDLMSDLEVADGM
                     GVIVRTAGSERSKTEIKRDFEYLMRLWDDIRELTLKSTAPALIYEEGNLIKRSIRDLY
                     TRDLEEVIVDGDEGYHTAKAFMKMLMPSHAKRVKPYRDPSIPLFHRYPVESQIDAIHS
                     PTVQLRSGGYIVINPTEALVAIDVNSGRSTKERNIEETALKTNVEAAEEIARQLRLRD
                     LAGLIVIDFIDMEEHRNQATVERKLKEGMRYDRARIQLGRISPFGLLELSRQRLRPSL
                     LEASTQPCPHCNGTGHVRSTESTALHVLRAIEEEGTRRRSAEIGVAVPTAVALYVLNH
                     KRDALVHAEQRNGFHVTISNDDSLVPPAFRLERVRSFTPAEIAALPPPVALAPPIEEE
                     EEDVAVEEDEEESPVVSAAAEPAEAPAEAPAERVESEQSQERHPRRRRRRRGRRGEEG
                     RDTSPPAAAPPGSDIAEPMHADDGSEPAEMLPAEAATAGDSAPAGEPGGPDENGNRKR
                     RRRGRRGGRRRRREHNGEGGEHHPSADEARPVAEAVEYLPEPERVEDVGAEMAEAAHA
                     TPAPAEAASGADAIPVGERGDKPSAVYAPRPPAAPAVTEERPRTVASAEVPTHDVTGP
                     PSNPKRGWWRRVLDS"
     gene            3536..>6018
                     /locus_tag="VGP50_17110"
     CDS             3536..>6018
                     /locus_tag="VGP50_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEV8029156.1"
                     /translation="MRVIKFLLKLGFGLGGAALVLGAGAGAFAIWYFGRDLPDYQALL
                     DYHPPIVSRVYAEDGRLLAEYATEKRVFVPAKEIPLLLIHAFLAAEDKNFYVHSGIDP
                     VAMLRAGATDVMRVITRKRPIGASTITQQVAKNFLVGSELSYERKIKEALVALRMEKV
                     LGKDRILELYLNDIYLGGGTYGVAAAALYYFNKSLDELSIEEAAFLAELPKAPNNYDP
                     LRHPEAAKGRRDWVIERMEEAGFITAQQGDQARATPITMRHRDEAETATASYFTEEVR
                     RDLLAHYGEKALYQSGLAVRTSLDPTLQAIADKSLRAGLIAYDRRHGWRGVVTHIDIA
                     GDWRARLVTVPPTAGSAEAGWSLGLVVAVGPEGATIGLSDGSRGLISLEEIRWARRAL
                     ADGELGPVPKQPGDVLKAGDVVLVEALPAAANKDEKLYALRQMPEVSGAVVALDPHTG
                     RVLALSGGFSYEMSQFDRAIQAHRQIGSAIKPFVYLTAMDHGFTPSSIMLDEPFVIDQ
                     GVGLPKWKPSNYTHKFYGPLTLRTGVEQSRNLITARLGEAVGLEPIGETIERFGIMEH
                     MPRQYAEVLGAGETTPLKLTTAYAMLVNGGKRITPTLIDRVQDRNGVTIYRADQRPCA
                     DCQNVEWQRQAPPELPDQREQIADPLSAYQIVSILQGVVERGTGRVVASVGKPLAGKT
                     GTTNDSDDTWFVGFSPDLAVGVFIGFDTPKSLGAHETGASVSAPVFRDFMAAALKNKP
                     AIPFRIPPGLHLVRVDLATGLPAHAGDKNVIFEAFKPGTEPTGESVAPAVAHGPISDD
                     EDAASVGGPTDEPTADSGTPVADAPDGPV"
BASE COUNT          855 a         2087 c         2038 g         1038 t
ORIGIN      
        1 gagacggtgc ggctctcgcg attgcaagag caatcgctgg cgccgccgcg gacgaacgcg
       61 ctttcgcgcg ttcgcctagg agtccaacac tcgacgccac catccgcgct tgggattgga
      121 aggcgggccc gtgacgtcgt gagtcggcac ctccgcggag gcgacggttc tcggccgctc
      181 ctcggtgacc gccggtgcgg ccggcggacg cggagcatag acggcggatg gcttgtcgcc
      241 ccgctcgccc accgggatgg catcggcgcc gctcgccgcc tcggccggag ccggcgtcgc
      301 gtgcgccgcc tcggccattt ccgcgcccac gtcctcgacc ctctccggtt ccggcagata
      361 ctcgaccgcc tcggcgaccg gccgcgcttc gtcggcggac ggatggtgct cgccgccctc
      421 gccattgtgc tcgcggcgcc gccgccggcc gcctctccgg ccgcggcggc ggcgcttgcg
      481 gttaccgttc tcgtcggggc cgcccggctc gccggcaggc gcagagtcgc ccgccgtcgc
      541 cgcctcggcg ggcaacatct ccgccggctc gctgccatcg tcggcgtgca tcggttctgc
      601 aatgtcgctc cccggcggtg cggcggccgg tggcgaggtg tcgcggccct cctcgccgcg
      661 tcgtccgcgg cggcgacggc ggcggcgcgg atgacgctcc tggctttgct cgctttcgac
      721 tcgctcggcc ggcgcttcgg ccggggcttc ggcgggctcg gccgcggcgc tcacgaccgg
      781 gctttcttcc tcgtcctcct cgacagcgac gtcctcctct tcctcctcga tcggcggcgc
      841 gagggcgacg ggcggcggga gcgccgcgat ctcggcgggc gtgaagctgc gcacccgctc
      901 gagccggaag gcgggcggca cgaggctgtc gtcattgctg atggtgacgt ggaagccgtt
      961 gcgctgctcc gcatggacca gcgcgtcgcg cttgtggttc agcacgtaga gtgcgaccgc
     1021 cgtcggcacg gcgacgccga tctcggccga gcgccgccgc gtgccctcct cttcgatggc
     1081 gcgcagcacg tggagcgcgg tcgactccgt cgagcggaca tggcccgtgc cgttgcaatg
     1141 cgggcagggc tgcgtcgagg cctcgagcag cgaggggcgc aggcgctggc gcgacagctc
     1201 gagaaggccg aaggggctga tgcggccgag ctggatgcgc gcgcgatcgt agcgcattcc
     1261 ctccttgagc ttgcgctcga ccgtggcctg gttccggtgc tcctccatgt cgatgaagtc
     1321 gatgacgatg aggccggcaa ggtcgcgcaa gcgcagttgc cgcgcgatct cctcggccgc
     1381 ctcgacattg gtcttgagcg ccgtctcctc gatgttgcgc tccttggtcg agcgccccga
     1441 gttgacgtcg atcgccacca gcgcctcggt cggattgatc acgatgtagc cgccggagcg
     1501 gagctgcacc gtcggcgaat ggatggcgtc gatctggctc tcgaccggat agcgatggaa
     1561 gagcgggatg ctggggtcgc gatagggctt cacgcgcttc gcgtggctcg gcatcagcat
     1621 cttcatgaag gctttggccg tgtgatagcc ttcgtcgcca tcgacgatta cttcctcgag
     1681 atcccgggta tagagatcgc ggatcgaccg cttgatgagg ttgccttcct cgtagatgag
     1741 cgcgggcgcg gtcgatttga gagtcagctc gcggatgtcg tcccagaggc gcatcagata
     1801 ttcgaaatcg cgcttgatct cggtcttgga gcgctcgctg ccggccgtac ggacgatcac
     1861 gcccatgccg tccgccactt cgagatcgct catgaggtcc ttgagccggc gccggtcggt
     1921 cacgctggtg atcttgcgcg aaacgccgcc gccccgcggc gtgttgggca tgagcacgca
     1981 atagcggccg gccagcgaca ggaaggtggt gagcgctgcg cccttgttgc cgcgctcttc
     2041 cttggtgact tgcaccagca tgatctgccg gcgcttgatc acctcctgga tcttgtagtg
     2101 acggtaatgc cgggcgcgct gccgctgcgc ctcctccatc tcgtcgccgc ccaccgtctc
     2161 gatcacgtcg ggccgcgttt cctcgccgcc attctcgtgc tcggcaggag ctcgctcgcc
     2221 gccattcgcg ccgttgccgt tgatctcgtg cggttcgtgg cgctccgctt cgccgttcgg
     2281 ctcgtcgccg gcgtcaccgt cgccgcccgc ggcggcgact tcggcctccg tctcgctcgt
     2341 cgactccgtg ccgatctcgc cgcccggctc ttccgccgca tcgcggcgct catagacgat
     2401 atcgggatca tgggcctcgg ccggggcggc gcgctccgcc tcgatttccg cggcgttcac
     2461 gatcttcatg acctcgtccg cctcggcgcc ttcaggctcg tcggcgccct ccgtcggccg
     2521 cggtgccgca tcgccccccg gcagctcctc cgcgccctcc tcgccgggct cggcgcggct
     2581 gggcggagcg cggtcgtggt aaggcatccg gtccgagacc gggattttgt agtagtcggg
     2641 gtggatctcg ttgaaggcaa ggaaaccgtg gcggtttccg ccatattcga cgaaggccgc
     2701 ctgcagcgac ggctcgacgc gggttacctt ggcgagataa atgtttccct tgatctgctt
     2761 tttggtcgat gtctcgaagt cgaattcttc gaggcggttg ttattgagca cgacgacccg
     2821 ggtctcctcc ggatgcgccg cgtcgatgag catgcgcttt gccatacggg cataactccg
     2881 agacgcgccg ttccgcccgg gcgaatcacg ggcggcggcg ccatcctggt tggggcgaag
     2941 ccggcagcgc ctccggtcaa tccgtcgaac ggcatctgag ttagggccat ggcggaactc
     3001 gtagtggctg ccgcgcccgg caagacgccg ggggttggct tcggggttca atactatccg
     3061 ctctgtcctg agcgcccgcc ggcggaagac cggcggcgtc tgcccagacg cccggccgtc
     3121 ccgccgcggc gcccctctgc ggccataagg gccggatcgg cggaccgcgg tatttcgcac
     3181 tgcccaggat acagtccgcg agccgccccg acaaccagta ctactgggcc gagcagtttc
     3241 cctttacccg agcagccgga tctggggcgg cagcgggtcc ggccatctgc cgttggagtc
     3301 cttgaccttg aacgcgatcg acaacggact cgtatcgccc aaaaggagag tagccgcccc
     3361 gactcgctcg tgccatcgcc gtgtcatcgg gttgccgagc gatgccgggg gaacaggccg
     3421 tgaaccacgg ccttgaaggc gccataaagc cgctacacct tatagcaacc tttatggctc
     3481 caccttgtcg gccggcggcg ttcaccgtca agggaagcga gcgatggcga gggacatgcg
     3541 ggtcatcaaa tttcttctca agctcggctt cgggctcggc ggcgctgcgc tggtcttggg
     3601 cgcgggtgcg ggcgctttcg ccatctggta tttcggccgg gatctccccg actatcaggc
     3661 gcttctcgac tatcatccgc cgatcgtctc gcgcgtctat gccgaggacg gccggctgct
     3721 cgccgaatat gccaccgaga agcgcgtctt cgtgccggcg aaggagatac cgctgctcct
     3781 catccatgcc ttcctcgccg ccgaggacaa gaatttctat gtccattccg gcatcgatcc
     3841 cgtggcgatg ctgcgcgccg gcgcgaccga cgtcatgcgc gtcatcacgc ggaagcggcc
     3901 gatcggcgcc tcgaccatca cgcagcaggt ggccaagaac ttcctcgtcg gcagcgagct
     3961 ctcctatgag cgcaagatca aggaagcgct ggtcgcgctg cgcatggaga aagtgctggg
     4021 caaggaccgc atcctagagc tctatctcaa cgacatctat ctgggcggcg gcacttacgg
     4081 cgtcgcggcc gcggcgctct actacttcaa caagtcgctc gacgagctct cgatcgagga
     4141 ggccgcgttc ctggcggagc tgcccaaggc gcccaacaac tacgacccgc tgcgtcatcc
     4201 cgaagcggcc aaaggccggc gtgactgggt gatcgagcgc atggaggagg cgggcttcat
     4261 cacggcgcag cagggcgacc aggcgcgcgc gacgcccatc accatgcgcc atcgcgacga
     4321 ggcggaaacg gcgacggcga gctacttcac cgaggaagtg cggcgcgacc ttctcgcgca
     4381 ttacggcgag aaggcgctct atcaatcggg cctcgccgtg cgcaccagcc tcgatccgac
     4441 gctgcaggcg atcgccgaca agtcgctgcg cgccggcctc atcgcctatg accgccgcca
     4501 tggctggcgc ggcgtcgtca cgcatatcga catcgccggc gactggcgcg cgcggctcgt
     4561 gaccgtgccg ccgacggccg ggtccgccga ggcggggtgg tcgctcggcc tggtggtcgc
     4621 ggtgggtccc gagggcgcaa cgatcgggct cagcgacggc agccgcggcc tgatttcgct
     4681 ggaggagatc cgctgggcgc gccgggcgct cgccgacggc gagctcggtc cggtgccgaa
     4741 gcagccgggc gacgtcctga aggccggcga cgtggtgctg gtcgaggcgc ttcctgccgc
     4801 cgccaacaag gacgagaagc tctacgcgct gcggcagatg ccggaagtgt cgggcgcggt
     4861 ggtggcgctc gatccgcata ccggacgcgt gctcgcgctc tcgggcggct tcagctacga
     4921 gatgagccag ttcgatcgcg cgatccaggc gcatcggcag atcggctcgg cgatcaagcc
     4981 cttcgtctat ctgacggcga tggatcacgg cttcacgccg tcgagcatca tgctcgacga
     5041 gcccttcgtc atcgatcagg gcgtcggcct gccgaaatgg aagcccagca actacacgca
     5101 taaattctat gggccgctga cgctccgcac gggcgtcgag cagtcgcgga atctcattac
     5161 ggcgcggctc ggcgaagccg tcgggctcga gcctatcggc gagaccatcg agcgcttcgg
     5221 catcatggag cacatgccgc ggcaatatgc ggaggtgctg ggggcgggcg agacgacgcc
     5281 gctcaagctc accacggcct atgccatgct ggtcaatggc ggcaagcgca tcacgccgac
     5341 cctcatcgac cgcgtccagg accggaacgg cgtcaccatc taccgcgccg accagcggcc
     5401 ctgcgccgac tgccagaatg tcgagtggca gcgccaggcg ccgcccgagc tgcccgacca
     5461 gcgcgagcag atcgccgatc ccttgagcgc ctatcagatc gtctcgatcc tgcagggcgt
     5521 cgtcgagcgc ggcaccggcc gcgtcgtcgc ctcggtcggc aagccgctcg ccggcaagac
     5581 cggcacgacc aatgattccg acgacacctg gttcgtcgga ttctcgccgg acctcgccgt
     5641 cggcgtgttc atcggcttcg acacgccgaa gagcttgggt gcgcatgaga ccggcgcttc
     5701 cgtctcggcg ccggtgttcc gcgatttcat ggcggcggcg ctcaagaaca agccggcaat
     5761 ccccttccgc atcccgccgg gcctccatct cgtgcgcgtc gatctcgcca ccgggcttcc
     5821 cgcgcatgcc ggcgacaaga acgtgatctt cgaggcgttc aagccgggca ccgagcccac
     5881 cggcgagtcg gtcgcgccgg ccgtcgccca tgggccgatc agcgacgacg aggatgccgc
     5941 ctcggtcggc ggcccgacgg acgagccaac cgcggatagc ggaacgcccg tggccgacgc
     6001 gcccgacggg ccggtcga
//
LOCUS       DASYNS010000559         1930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2954976, whole genome shotgun sequence.
ACCESSION   DASYNS010000559 DASYNS010000000
VERSION     DASYNS010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2954976"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VGP50_17115"
     CDS             <1..559
                     /locus_tag="VGP50_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029157.1"
                     /translation="LAAAAMLFVVVWAGGILPGTHRGASPAKTGVSSIPATQALDQAV
                     YSYETFETAFRPTVPSGSLADIAMAYGSAEMPDMMWNFQFAGYGVVGGRIDQLPDGNP
                     VTYTLYHGRNSDILCTRYKASDFAVPPGAVGEMHGHLLYQYKGYSLCVTVSEQGHFIC
                     VLTTSEPMDELRHDISLARAFSIVR"
     gene            complement(561..>1930)
                     /locus_tag="VGP50_17120"
     CDS             complement(561..>1930)
                     /locus_tag="VGP50_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029158.1"
                     /translation="LRALAAALDLRLVRYASDLLQARARRAAGAPGASPQAPPSALAP
                     ADADSGEADAGGDAADAGLAVRIARFGAEAPELVGIAAPIARISELLENDRIELPSEE
                     TEIDEPAEPPAAVVRNFLIRFSARHTLALTAAFLIGVWDNNPALHAAIWLLMLGGPPS
                     HGATVRKFTMRAIGASGALALAALATIVVAPNFTSPGPYMAAIFVGALLMAYIGEGGG
                     ILSYLAIGGTAFVIGYGGPGPRADVLGSIWSLWGISLGMMIRAAVSLLWRERASRTLA
                     EQFQAPLAAMLELVKEGGGYDESRRRAAAERSAVAGIAMMLTVANDAMLEGRGAEIDG
                     DQLVNALDTLRRLAFALGNLTRVRPAGIAMSADVSAELTDAIRMRLESWLCSLRDQTE
                     SGVASRAPLRKMVAEANAPELRPLGDERTAAPGIEMSYVVAAERGIRLIQMLEHQLAA
                     VSRAV"
BASE COUNT          333 a          663 c          623 g          311 t
ORIGIN      
        1 gctcgccgcg gccgcgatgc tgttcgtcgt ggtgtgggcg ggcggaatcc tgcccgggac
       61 gcatcgaggc gcgtcgcccg ccaaaactgg agtcagctcg atcccggcca cccaggcgct
      121 cgatcaggcc gtgtacagct acgagacctt cgagaccgct ttccggccca ccgtgccgtc
      181 gggctcgctc gcggatatcg cgatggccta cggctcggcc gagatgccgg acatgatgtg
      241 gaatttccag ttcgccggct atggcgtggt cggcggacgg atcgatcagc tacccgatgg
      301 caatcctgtc acctacacgc tctatcatgg ccgcaacagc gacatcctgt gcacgcgcta
      361 caaggcgagc gactttgccg tgccgccggg cgccgtcggc gagatgcacg gccatctgct
      421 ctaccaatac aagggctata gtctctgcgt aacggtgtcg gagcagggcc acttcatctg
      481 tgtgctgacg acgagcgagc cgatggacga gctacgccac gacatctcgc tcgcccgcgc
      541 gttcagcatc gtgcgctagc ttagacagcg cgcgaaaccg cggccagttg atgctccagc
      601 atttgtatca gccggatccc gcgctcggcg gccacgacgt acgacatctc aatcccgggc
      661 gcagcagtac gctcgtcacc caatgggcgc agttcgggcg cgttggcttc ggcaaccatc
      721 ttcctgagcg gcgcgcggct tgcgacgccg gactccgtct gatcgcgcag gctgcatagc
      781 cacgattcca gacgcatccg gatcgcgtcg gtcaactccg cgctcacatc ggcgctcatg
      841 gcaattccgg ccggtcggac gcgggtcaga ttacccaggg cgaaggccaa gcggcggagt
      901 gtgtcgagcg cgttgaccaa ctggtcaccg tcgatttcag cgccgcgacc ttcgagcatc
      961 gcatcattcg cgactgtcag catcatggca atgccggcga cggccgacct ctcggctgcg
     1021 gcgcgccggc ggctctcatc gtagcccccg ccctctttca caagttcgag cattgccgcg
     1081 agcggcgcct ggaactgctc ggcgagcgtg cggcttgccc gctcgcgcca caggagagaa
     1141 acggcggcgc ggatcatcat gccgaggctg atgccccaca ggctccagat cgagccgagc
     1201 acgtcggcgc gcggtcccgg gcctccgtag ccgataacaa aggccgtgcc gccaatcgcg
     1261 agatacgaaa ggattcctcc accctcgccg atataagcca tcagcaaagc gcccacaaag
     1321 atcgcggcca tgtagggtcc gggcgaggtg aagttgggcg caacgacaat tgtggcgagc
     1381 gccgccagcg cgagagctcc tgacgcacca atcgcacgca tcgtgaattt gcgcaccgtg
     1441 gctccatggc tgggcgggcc gccgagcatc agcagccaga tcgccgcgtg caacgccgga
     1501 ttgttgtccc aaaccccgat cagaaacgcg gccgtcagtg cgagcgtatg tcgagcgctg
     1561 aagcggatga ggaaattccg tacgacggca gccggtggct ccgccggctc gtcgatttcg
     1621 gtctcttcgg atggaagctc gattcgatcg ttctcgagca gctcgctgat ccttgctatc
     1681 ggcgcggcaa ttcccaccag ctcgggagcc tccgcgccga agcgcgcgat ccgcacggcg
     1741 aggccggcgt cggcagcgtc gccgccggcg tcggcctcgc cgctatccgc atccgccggc
     1801 gccagcgccg acggaggcgc ctgcgggctc gcgcccggcg cgcctgcggc gcgccgggcg
     1861 cgagcctgca gcaaatccga ggcgtagcga acgagacgca gatcgagcgc cgccgcgagc
     1921 gcgcgcaatt
//
LOCUS       DASYNS010000560         7387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4823047, whole genome shotgun sequence.
ACCESSION   DASYNS010000560 DASYNS010000000
VERSION     DASYNS010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4823047"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VGP50_17125"
     CDS             complement(<1..603)
                     /locus_tag="VGP50_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008890127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV8029159.1"
                     /translation="MAMLKGGLIQMSFKGDASMSPAHIRERMIAAHLPLIDAAGREGV
                     QVLCLQEVFTQPYFCPSQDAKWYAAVEHIPDGPTTALMQELARKHSMVIVAPIYEEEI
                     TGVYYNAAAVIDADGRYLGKYRKTHIPQVAGFYEKFFFKPGNSGYPVFQTAYCKLGVY
                     ICYDRHFPEGWRALALNGAEYIVNPSATVAGLSQYLWELEQ"
     gene            complement(616..2067)
                     /gene="hydA"
                     /locus_tag="VGP50_17130"
     CDS             complement(616..2067)
                     /gene="hydA"
                     /locus_tag="VGP50_17130"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEV8029160.1"
                     /translation="MAILIRGGTVVNADASQRADVLVDHGVVKAVGADLAAPGGAEIV
                     DAGGAYVMPGGIDPHTHMELPFMGTVTSEDFFSGTSAAAAGGTTMVIDFVIPSPQEKL
                     LEAFRKWRGWSEKAATDYSFHVAVTWWDESVPRDMGTLVAEHGVNSFKHFMAYKNAIM
                     ADDEILVKSFLRARELGALCTVHAENGELVYHLQQELLKSGINGPEGHPLSRPPAVEG
                     EAANRAIRLAEVLGVPLYIVHNSCREAVEAIARARGEGQRVFGEVLAGHLLVDDSVYW
                     NEDFATAAAYVMSPPFRSKEHQAVLWQALQAGTLQTTATDHCCFCAPQKAMGRGDFSK
                     IPNGTGGIEDRMHVLWHHGVGTGRLTPNEFVAATSTNAAKIFNIYPRKGVIAVGSDAD
                     LVVWDPKKTRRISAKTHHQKIDFNIFEGMEVEGVNVVTLSQGKIVWRDGDLRTVKGAG
                     RWVDRPPFPAYYTALLKKETLERRRPALRHAAE"
     gene            2172..3497
                     /locus_tag="VGP50_17135"
     CDS             2172..3497
                     /locus_tag="VGP50_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEV8029161.1"
                     /translation="MALSARAPNDLGPYWMPFTANRQFKSQPRLLVAAKGHHYTAADG
                     RRILDGTAGLWCCNAGHGHPKIVAAIQAQAAEMDYAPAFQMGHPKAFELASRVAALAP
                     GDLDHVFFTNSGSEAVDTALKMAIAYHRLKGEASRTRLIGRERGYHGVGFGGISVGGM
                     GNNRKSFGPMLVGVDHLPHTYDRARQAFSRGEPDWGTHLADELERLVALHDASTIAAV
                     IVEPVACSAGVLVPPKGYLKRLREICTKHGILLILDEVITAFGRLGTRFAADYFDVMP
                     DLITTAKGLTSGAVPMGAVIVRKGIYDAFMTGPEHAVELFHGYTYSGHPLACAAGLAA
                     LEVYAEEKLFENARGLASYWQEAAHGLKGLPHVIDIRNIGLVAAVELEPIAGQPAKRA
                     FDAFLRAFETGLLIRTTGDIIALSPPLTITTKEIDEIFGKLSDVLKATA"
     gene            complement(3612..4298)
                     /locus_tag="VGP50_17140"
     CDS             complement(3612..4298)
                     /locus_tag="VGP50_17140"
                     /inference="COORDINATES: protein motif:HMM:NF019961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV8029162.1"
                     /translation="MSHVEVSAGGKRRVARSVARTNGRAAAIRERNEAKLLKAAQAVF
                     AKKGFSGATTAEIARRAGIPKPNLHYYFRTKRLLYAAVLDGILDIWVDALGEIRAEAE
                     PADALRRYIARKMESSRSLPLPSRLWAMELLGGGRHVQPFLKGRLKRLVEQKSDVIRN
                     WIAAGKMDAVDPAHLLFGLWAMTQTYADFAAQISAVLGKDRLDKPVFAAATATLTAVV
                     LKGTGAEKAR"
     gene            4472..5830
                     /locus_tag="VGP50_17145"
     CDS             4472..5830
                     /locus_tag="VGP50_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8029163.1"
                     /translation="MAMARGDRPDVAAGRLPDEAYRENFADIHPPLDRKAALVEANRC
                     LFCYDAPCIEACPTGIDIPGFIKRIASDNVEGAGMRILDANIFGGVCARVCPTEILCE
                     GACVRVAQEGKAVEIGRLQRYAVDPILESPTHPFRRAAPTGKRVAVVGGGPAGLACAH
                     RLALLGHDVTVIEARERLGGLNEYGVAEYKVAEHFARAEVDFILGIGGISVRCATRLG
                     RDVTLAELRSGFDAVFLGMGLAGVNALDCEGETLTGVFDAVDYIARLRQAEDFSGLPV
                     GRRVVVIGGGNTAIDIAVQTKRLGAEDVTLVYRRGPDAMSATDREQHFAQVNGVKIKH
                     WARPVQTRGHNGEVRDAVFEYTQIADGLVAGTGEHFALPADMVFKAIGQVFVADPAKD
                     GAQDMLELEQGRITVDGERRTSLAKVWAGGDCIRGGEDLTVRAVEDGKRAAQSIHRAL
                     MG"
     gene            5842..7122
                     /gene="preA"
                     /locus_tag="VGP50_17150"
     CDS             5842..7122
                     /gene="preA"
                     /locus_tag="VGP50_17150"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEV8029164.1"
                     /translation="MADLSAQFAGIKSPNPFWLASAPPTDKAYNVVRAFKAGWGGVVW
                     KTLGEDPPIINTTSRYGGLSYHGNRVAGFTNIELITDRPLDINLREIKEVKRDWPDRA
                     LIVSLMVPCDETSWQRILTQVEDTGADGIELNFGCPHGMSERGMGSAVGQVPDYVEMV
                     TRWCKQATRMPVIVKLTPNVTNILGPARAAVRGGADAVSLINTLNSIIAVDLEQMAPV
                     PVVDGKGTHGGYCGPAVKPIALHMVAEIARDPECRRIPIAGIGGIETWRDAAEFMALG
                     AGVVQVCTAAMHYGFRIVDEMADGLGRWMRDKGYDGIAAFCGKAVPNVTDWQFLNLNY
                     QIVADIDQDLCIKCGLCHIACEDTAHQAIAALRVEGERRYEVIEKECVGCNLCLFVCP
                     VEDCITMRRVDDGKSRLDWTQHPNNPLRPSTSSG"
     gene            7196..>7387
                     /locus_tag="VGP50_17155"
     CDS             7196..>7387
                     /locus_tag="VGP50_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541207.1"
                     /note="allantoate amidohydrolase and N-carbamoyl-L-amino
                     acid amidohydrolase are very similar; the allantoate
                     amidohydrolase from Escherichia coli forms a dimer and
                     binds zinc ions for catalytic activity and catalyzes the
                     conversion of allantoate to (S)-ureidoglycolate and
                     ammonia; carbamoyl amidohydrolase from Bacillus sp.
                     converts N-carbamoyl amino acids to amino acids, ammonia,
                     and carbon dioxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEV8029165.1"
                     /translation="MTAPGNLRINGERLWQSLMEMAKIGATEKGGSKRLALSDLDRAG
                     RDLFVRWCKEAGCSVTVDRM"
BASE COUNT         1250 a         2380 c         2504 g         1253 t
ORIGIN      
        1 ctgctccagc tcccagagat actggctcag gccggcgacg gtggccgagg gattgacgat
       61 gtattcggcg ccgttgagcg cgagcgcgcg ccagccttcg gggaaatgcc ggtcgtagca
      121 gatatagacg ccgagcttgc aataagcggt ctggaagacg ggatagccgc tgttgcccgg
      181 cttgaagaag aatttctcgt agaagccggc gacttgcggg atgtgcgtct tgcggtactt
      241 gccgagatag cggccgtcgg cgtcgatcac ggcggcggcg ttgtaataga cgccggtgat
      301 ctcctcttcg tagatgggcg cgacgatcac catcgaatgt ttgcgcgcga gctcctgcat
      361 gagcgcggtc gtggggccgt cgggaatgtg ctcgacggcg gcataccatt tcgcgtcctg
      421 gctcgggcag aaatagggct gggtgaagac ctcctggaga cacaggacct ggacgccctc
      481 gcgccccgcc gcgtcgatca gcggcagatg cgcggcgatc atgcgctcgc ggatatgcgc
      541 gggcgacatc gacgcgtcgc ccttgaagct catctgaatg aggccgccct tgagcatggc
      601 catgggccgc caccgttact cggcggcgtg tcgcagcgcc ggccggcgcc gctccagcgt
      661 ctccttcttg aggagcgcgg tgtaataggc cgggaacggc ggacggtcga cccagcgtcc
      721 cgcgcctttc accgtgcgaa gatcgccgtc gcgccagacg atcttgccct gcgacagcgt
      781 cacgacgttg acgccttcga cttccatgcc ttcgaagatg ttgaaatcga tcttctgatg
      841 atgagtcttg gcggagatgc ggcgcgtctt cttcgggtcc cagacgacga ggtcggcgtc
      901 ggagccgacg gcgatcacgc ctttgcgcgg atagatgttg aagatcttgg cggcgttggt
      961 cgaggtggcc gcgacgaact cgttgggcgt gaggcggccg gtgccgacgc cgtgatgcca
     1021 gagcacatgc atgcggtcct cgatgccacc ggtgccattc gggatcttgc tgaaatcgcc
     1081 gcgccccatg gccttttgcg gcgcgcagaa gcagcagtga tccgtcgccg tggtctgcag
     1141 cgtgccggcc tggagcgcct gccacagcac cgcctgatgc tccttggagc ggaagggcgg
     1201 gctcatgacg taagcggcgg ccgtggcgaa atcctcgttc cagtagacgc tgtcgtcgac
     1261 gaggagatgg cccgccagca cttcgccgaa gacgcgctgg ccttcgcccc gcgcccgcgc
     1321 gatcgcttcg accgcctcgc ggcaggaatt gtggacgatg tagagcggaa cgcccagcac
     1381 ctcggcgagg cggatggcgc ggtttgcggc ctcgccctcg acggcgggcg ggcgcgacag
     1441 cggatggccc tcggggccgt tgatgcccga tttcagcaat tcctgctgga gatggtagac
     1501 gagttcgcca ttctcggcat ggacggtgca gagcgcgccg agctcgcgcg cccgcaagaa
     1561 gcttttcacg agaatctcgt catccgccat gatggcgttc ttgtaggcca tgaaatgctt
     1621 gaagctgttg acgccatgct cggcaaccag cgtccccatg tcgcgcggca cgctctcgtc
     1681 ccaccaggtt acggcgacgt ggaagctgta atcggtcgcg gccttctcgg accagccgcg
     1741 ccatttcctg aaggcctcga gcagcttctc ctgcgggctc ggaatcacga agtcgatgac
     1801 catggtggtg ccgccggcgg cggccgccga ggtgccggag aagaaatctt ccgacgtcac
     1861 cgtgcccatg aagggcagtt ccatatgcgt gtgcgggtcg atgccgccgg gcatcacata
     1921 tgcgccgccg gcgtcgacga tctcggcgcc gccgggtgcg gcgagatcgg cgcccaccgc
     1981 cttgacgacg ccgtgatcga cgaggacatc ggcgcgttgg ctggcatcgg cattcaccac
     2041 cgtaccgccg cggatcagga tggccatggc atcctccttg ggagagatct tgcgagccta
     2101 cgccacactt gaccattcga tcaagcaagc ccgaggatgg agcaaccgcc cttcgctacg
     2161 aggagacgag catggccctg agcgcccgcg ccccgaacga tctcggcccc tactggatgc
     2221 cgttcacggc gaaccgccag ttcaagtcgc agccgcgcct cctggttgca gccaaggggc
     2281 accactacac ggccgcggac gggcggcgga tcctcgacgg cacggcgggg ctgtggtgct
     2341 gcaatgccgg gcacggccat cccaagatcg tcgccgcgat ccaggcgcag gcggcggaaa
     2401 tggattatgc gccggcgttc cagatgggcc atcccaaggc cttcgagctc gcttcgcgcg
     2461 tggcggcgct ggcacccggc gacctcgacc atgtgttctt caccaattcg ggctcagagg
     2521 cggtcgacac ggcgctcaag atggcgatcg cctatcaccg cctcaaaggc gaagcgtcgc
     2581 gcacgcgtct catcggccgc gagcgcggct atcacggcgt cggcttcggc ggcatatcgg
     2641 tcggcggcat gggcaacaat cgcaaatcct tcgggccgat gctcgtcggc gtcgaccatc
     2701 ttccgcacac ctatgaccgc gcgcgccaag ccttctcgcg cggcgagccc gactggggca
     2761 cgcatctcgc cgacgagctc gagcgcctgg tggcactcca cgacgcctcg accatcgccg
     2821 ccgtgatcgt cgagccggtc gcctgctcgg ccggcgtcct ggtgccgccc aagggctatc
     2881 tgaagcgcct gcgcgagatc tgcacgaagc acggcattct cctcatcctc gacgaggtca
     2941 tcaccgcctt cggccggttg ggcacgcgct ttgccgccga ttatttcgac gtgatgccgg
     3001 atctcatcac cacggcgaaa gggctgacct cgggcgcggt gccgatgggc gcggtgatcg
     3061 tgcgcaaagg catctacgac gcgttcatga ccgggccgga gcacgccgtc gagctcttcc
     3121 acggctacac ctattcgggc catccgctgg cctgcgcggc cgggcttgcc gcgctcgagg
     3181 tttatgccga ggaaaagctc ttcgagaatg cgcgcgggct ggcgtcctat tggcaggaag
     3241 cggcgcatgg cctcaaaggc ctgccgcatg tgatcgacat ccgcaatatc ggcctcgtcg
     3301 ccgccgtcga gctcgagccg atcgcgggac agccggcgaa gcgcgctttc gatgcgttcc
     3361 tgcgcgcctt cgagaccggg ctcctcatcc gcaccaccgg cgatatcatc gccctctcgc
     3421 cgccgctcac gatcaccacg aaagagatcg acgagatctt cggaaaattg agcgacgtgc
     3481 tgaaggcgac ggcgtaagac gcaggaaaag aaagaggaac gcagaggacg ccgaggatcc
     3541 gcggagggcg caaaggagaa tcatccctct gcgtcctccg cgtcgccttt gcgtcctctg
     3601 cgttccctac ttcaccgcgc cttctcggcc cctgtccctt tgagcacgac cgccgtcaag
     3661 gtcgccgtcg cggcggcgaa gacgggtttg tcgaggcggt ccttgccgag gacggcggag
     3721 atctgtgcgg caaaatcggc gtaggtctgg gtcatggccc agaggccgaa gaggagatgc
     3781 gcgggatcga cggcgtccat tttgccggcg gcgatccagt tgcggatgac atcgctcttc
     3841 tgctcgacaa gacgcttgag ccggcctttg agaaagggct ggacgtggcg gccaccgccc
     3901 agaagctcca tcgcccagag gcgcgagggc agcggcaggc tgcgcgagct ctccatcttg
     3961 cgggcgatgt agcggcggag cgcgtcggcg ggctcggcct cggcgcggat ttcgcccagc
     4021 gcatcgaccc agatgtcgag gatgccgtcg agcaccgcgg cgtagagcag gcgcttcgtg
     4081 cgaaaataat aatggagatt gggcttggga atgcccgcgc gacgcgcaat ctcggcggtg
     4141 gtggcgccgc tgaagccctt tttggcgaag accgcctggg ccgccttcag cagctttgct
     4201 tcgttgcgct cgcggatagc ggcggcgcgg ccgttggtcc gggccacgct ccgcgcgaca
     4261 cggcgcttgc cgccggcgct cacctccaca tgcgacatgc tgcggccgct ccgatctcaa
     4321 acgcatcgat ttaggcgcgg cgtgaaggcg ccgtcaaagg aaattgccgt tgcttcgcgc
     4381 ttgaccgatc ggtcaatcgg ctcatagagt tccgggacat gacggcgcgc tggcctcttc
     4441 cgccggatcc gcgccgggga tgacggaagc gatggcaatg gcgcgagggg accgtccgga
     4501 cgtcgcggcg gggcggctgc cggacgaagc ctatcgcgag aatttcgccg acattcaccc
     4561 gccgctcgac cgcaaggccg ctctggtcga ggccaatcgc tgcctcttct gctacgacgc
     4621 gccctgcatt gaggcctgtc ccacgggcat cgacatcccg ggcttcatca agcggatcgc
     4681 cagcgacaat gtcgaagggg ccggcatgcg cattcttgac gccaatatct tcggcggcgt
     4741 ctgcgcacgc gtctgtccga cggagatcct gtgcgagggc gcttgtgtgc gcgtcgcgca
     4801 ggaaggcaag gcggtcgaga tcggccggct ccagcgctat gccgtcgatc ccattcttga
     4861 atcgccgacg catccgttcc gccgcgccgc gccgacgggg aagcgcgttg ccgtggtggg
     4921 cggcgggccg gccggccttg cttgcgccca tcgcctggcg ctgctcggtc acgacgtcac
     4981 ggtgatcgag gcgcgcgagc gcctgggcgg gctcaacgag tatggtgtcg ccgaatacaa
     5041 ggtggcggag catttcgccc gcgccgaggt cgatttcatc ctcggcatcg gcggcatcag
     5101 cgtgcgttgc gcgacccgcc tcggccgcga cgtcacgctc gccgagctgc gaagcggctt
     5161 cgacgccgtg ttcctcggca tggggcttgc aggagtgaat gcgctcgact gcgagggaga
     5221 gacactgacg ggcgtcttcg atgccgtcga ctatatcgcg aggctgcgcc aggccgagga
     5281 tttctcaggc ttgccggtgg ggcggcgcgt cgtcgtgatc ggcggcggca acaccgccat
     5341 cgacatcgcg gtgcagacga agcggctcgg cgccgaggac gtgacgctgg tctatcgccg
     5401 cggccccgac gccatgagcg cgaccgaccg cgagcagcat ttcgcccaag tgaacggcgt
     5461 caagatcaaa cattgggcaa ggccggtgca gactcgcggc cataacggcg aggtgcgcga
     5521 cgccgtcttc gaatatacgc agatcgcgga tggccttgtc gccggcaccg gcgagcactt
     5581 cgcgctgccc gcggacatgg tgttcaaggc gatcggccag gtcttcgtcg ccgatcccgc
     5641 gaaggacggc gcgcaagaca tgctcgagct ggagcagggt cgcatcaccg tcgatggcga
     5701 gcggcggaca tcgcttgcca aagtctgggc gggcggcgat tgcatccgcg gcggcgagga
     5761 tctgacggtg cgggctgtcg aggacggcaa gcgtgcggcg caatcgattc accgcgcgct
     5821 catgggatga aggagagcgc catggccgat ctcagcgcgc agtttgccgg gatcaagtcg
     5881 ccgaacccgt tctggctggc gtcggcgcca ccgaccgaca aggcctacaa cgtcgtccgc
     5941 gccttcaagg cgggatgggg cggcgtagta tggaagacgc tgggcgagga cccgccgatc
     6001 atcaatacga cgtcgcgcta tggcgggctc tcctatcacg gcaaccgcgt ggcgggcttc
     6061 accaatatcg agctcatcac cgaccggccg ctcgacatca atctccgcga gatcaaggaa
     6121 gtgaaacgcg actggccgga ccgcgcgctg atcgtgtcgc tgatggtgcc gtgcgatgag
     6181 acgagctggc agcgcatcct gacacaggtc gaggacaccg gtgccgacgg catcgagctc
     6241 aattttggct gcccgcacgg catgtccgag cgcggcatgg gctcggccgt gggccaggtg
     6301 ccggactacg tcgagatggt gacgcgctgg tgcaagcagg cgacgcgcat gccggtcatc
     6361 gtcaagctca cgcccaacgt caccaacata ttggggccgg cgcgcgcggc ggtgcgcggc
     6421 ggcgccgacg cggtgagcct catcaatacg ctcaattcga tcatcgcggt cgatctcgag
     6481 cagatggcgc cggttccggt ggtcgacggc aaaggtacgc atggcggcta ttgcggcccc
     6541 gcggtgaagc cgatcgcgct gcacatggtg gcggagatcg cgcgcgatcc cgaatgccgc
     6601 cgcatcccga tcgccggcat cggcggcatc gagacctggc gcgacgccgc cgaattcatg
     6661 gcgctgggcg ccggcgtggt gcaggtctgc accgcggcga tgcattacgg attccgcatc
     6721 gtcgacgaga tggcggacgg gttgggccgc tggatgcgcg acaagggcta tgacggcatc
     6781 gcggcgttct gcggcaaggc cgtgcccaac gtcacggatt ggcagttcct caacctcaat
     6841 taccagatcg tcgccgacat cgaccaggat ctctgcatca aatgcgggct ctgccacatc
     6901 gcctgcgagg acacggcgca tcaggcgatc gccgcgctgc gcgttgaggg cgagcgccgc
     6961 tacgaagtga tcgagaagga atgcgtcggc tgcaatctct gcctcttcgt ctgcccggtc
     7021 gaggattgca ttaccatgcg gcgcgtcgat gacggaaaga gccggctcga ctggacgcag
     7081 catcccaaca accccctacg tccttcgaca agctcaggat gaggtttttc tttatgccat
     7141 ccaaaattct ccctcatcct gagcctgtcg aaggacgcaa ggcgggcatg cagccatgac
     7201 agcgcccggc aatctcagga tcaacggcga gcgcctgtgg cagagtctca tggagatggc
     7261 gaagatcggc gcgaccgaga agggcgggag caagcgcctc gcgctcagcg atctcgaccg
     7321 cgccgggcgc gacctcttcg tgcgctggtg caaggaagca ggctgcagcg tcaccgtcga
     7381 ccgcatg
//
LOCUS       DASYNS010000561         3434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5447871, whole genome shotgun sequence.
ACCESSION   DASYNS010000561 DASYNS010000000
VERSION     DASYNS010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5447871"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            752..1066
                     /locus_tag="VGP50_17160"
     CDS             752..1066
                     /locus_tag="VGP50_17160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEV8029166.1"
                     /translation="MTELRVTRPIKRAPSHPGELMREILDDHVRLTVAGAARRMDVSR
                     QSLHAVLSGASAVTADMALRFGKLVGGAPELYVSMQMAHDLWQARQKLRDTLERIEPV
                     AS"
     gene            complement(1382..1909)
                     /locus_tag="VGP50_17165"
     CDS             complement(1382..1909)
                     /locus_tag="VGP50_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029167.1"
                     /translation="MLLAVELLLEMGRNSAAEAQLWRAFEKAPNLDLYGRLRALGGEM
                     ARERAVALLEARPVKESPMLWHSADLLVDILIAEKMFDAAWAAVRRHRVSPRKQGVLA
                     AAIEATHPREALGIYAEQVEGLAGGSGGTTDYAQAAELIARMATLRSAGEQAAYVAAL
                     KLRFGRRRNLMKLLA"
     gene            3154..>3434
                     /locus_tag="VGP50_17170"
     CDS             3154..>3434
                     /locus_tag="VGP50_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEV8029168.1"
                     /translation="MRRADRLFQIIQILRRRRRPVTAAAIAAELERSLRTVYRDIADL
                     MAQSVPIRGEAGIGYVLDRGFDMPPLMLTADEIEAAMLGAQWVMRRTDP"
BASE COUNT          583 a         1144 c         1129 g          578 t
ORIGIN      
        1 cgacgctgct gacagcaccg tgtcaggagc gtccgggcgc gcgcgaaagc agattgggcg
       61 cggctcggca aatggcgcag cctgcggccc cacggcggaa accgtcgaga cgcggccctc
      121 atcgcttcag cgccgcgtcg cagaagggcc agggtcaaaa aaccgataac ggccgggctg
      181 cttccgccgc ggttccagaa gttggcccta tggagcccgg tcgtcggaga tgtgccaagc
      241 ggcaggtcac cgagatgcca accgtaatgt ggcacggatg gcgccaaagg cgacgtggcc
      301 tgcgccgccg gatcgacaaa gtcgttcggg cgtctcgtgc ccgtcgtgct ttggcggtca
      361 ggcaggttgc ttatatgtaa tgcatcgctt gacaccatgt gcggtgatac cttacacttg
      421 cttcatgatc gaaggctttc gcagcaaagg tctcaaggag ctcttcgaga ctgggtcgtc
      481 cgcgaaagtg gcaaccaatc tgcagcgacg gattgtcgtc agactcgatg ctctcgacgc
      541 ggccactgcg ttagccgagc tgaatcagcc gggctttaac cttcacatgc tgaaggggaa
      601 accggtgcgt cattcgatac atgtcaacgg accctggtgc atcactttca aatgggacaa
      661 gggcaaagcc aggagggtag atctcgaaca ataccactga gcagaagcat gatatgtgcc
      721 cggcccggtg cggctggcgg aggatagtga tatgacggaa cttagagtta ctcgcccgat
      781 caaacgggcg ccctcgcatc ccggcgagct catgcgagag attttggatg atcatgtgcg
      841 gctgactgtt gccggggccg cacggcgcat ggacgtgagt cggcagtcgc ttcatgcggt
      901 gctctcgggc gcgagtgccg taacggccga catggcgctt cgtttcggta agcttgtcgg
      961 tggcgcgccg gagctctatg tgagtatgca gatggcgcat gacctctggc aggcgcggca
     1021 gaaattaaga gacacgctgg agcgcatcga gcctgtcgcc tcgtaactgc gggcggtcag
     1081 acctgccgca gtgcgtcaaa cgcagcactt gcagcgcgtt tttgacgcgt taggttcacc
     1141 tcgacaattt ccgtggaagc acggatgggg gatccttcgg cgatatcatg cgatgtccag
     1201 cttcggcagt ggtgcggatt ggcaagcgac gcgtgaccta tggccagctc cgcgagcgcg
     1261 aaagcacgcg cgaacgcatc agggtaaacc gccagctcct tcgaggacgt tgacactgtc
     1321 aaaattgttc ccgagggacc tgaggcaaaa ccggcgctgg cggggccaac ccgctccgcc
     1381 ctcaggccaa cagcttcatc aagttgcggc ggcggccgaa gcgcagcttg agtgccgcga
     1441 cataggccgc ctgctcgccg gcactgcgca gcgtcgccat gcgcgcgatc agctcagccg
     1501 cctgcgcgta gtcggtcgtg ccgccgctgc cgccggcgag cccctcgacc tgttcggcat
     1561 agatgccgag tgcctcgcgc ggatgcgtcg cttcgatcgc cgcggcaagc acgccctgtt
     1621 tgcgcggcga gacgcgatgc cggcggacgg ccgcccaggc ggcgtcgaac atcttctccg
     1681 cgatgaggat gtcgacgaga agatcggccg aatgccagag catcggcgac tccttgaccg
     1741 ggcgcgcctc gagcagcgcg accgcgcgct cgcgcgccat ctcgccacca agcgcgcgca
     1801 gccggccgta gagatcgagg ttgggcgcct tctcgaaggc acgccagagc tgcgcctccg
     1861 ccgcgctgtt ccggcccatc tcgaggagaa gttctaccgc gagcagcacc aacctctggt
     1921 ctggccgccc gtcctcgaag cttctcccat tccgcggcgg cgaggcggcg atattcggcg
     1981 aggccttcct cgccaaggac ctcggcataa agcccggccg cgccggcaaa cgtaccgtag
     2041 tcatccgtcg tctcgcgctc gaagagatcg cgggcgagcg ccaccggatc gggtcgcgcc
     2101 acgctggcgg cggcgcgatg gatctcgctc gcgcgctcca gcaatgcatg gcattccccg
     2161 tccgagtcgt cgatctcctc gatcgcgtcc gcgatcccgt cgatggcgcg ctcggcgagg
     2221 tcgagcgcca gcgcggcgcg cggcgtctgg gccagctcgg cgacggcgtc gagcgcggcc
     2281 tcgacctcgg tggcccagcc tgccgctccg cggtagccga tgtagccgcg ggtctccgtc
     2341 gccgtatcga tcgcctcgcg caggcgcgcc gcgagcgtcc cgtcgtcggc atgggccatg
     2401 gccgcggccg tatcgaggcg gctgaacaag gccaagtcgc gctcggccat cgccacgatc
     2461 atctccacga gcgcgtcgac gtccttttcc cggaggtaag tgcgaatgcg cgcgagagcg
     2521 ccagcgcctt gtggatcgcc tgccgcatca ttggccgcga gcgctaccgc gaccatgtgc
     2581 ttgcagaagc cccagtccga aaaggcgcga caggaacatt ggcccccgat gtcggcgccg
     2641 gtcccggtaa gctcggtgcg gtaggtctcc gtccccacga cccgtgccaa cacccgctcc
     2701 ggctcgatgc taaggagggt gacctgcccc tctgcgtgat agacctcgcc gcgcgcgaac
     2761 gccttctccc cggccagctc gcgcaagccg tcgatatcga agcgtggttg ctggctcgtc
     2821 tcgatccgcg cttccgccgc cattcccttc tccgactcgt ccgcgccacg atcggagcag
     2881 gttatggcaa agcccggcag cgcggcaccg tgatttccgt cgactttccc ggcagcgtca
     2941 aaaaaccgat acaacgcatt gacggagcgt ggcgctcgcc cgacgcgcgc tgcgcacagc
     3001 gcggcgcgca gccttccacg atcgcccgcc cgcgccagtt ttgcggtcct ccgagtcgca
     3061 ggatcggaga cgtcgtcagg caggcttgcc ttgcgatacc ctccggagcg ataacctcgg
     3121 tcgtatggca gcatctgtca gcagggaggg gcgatgcgcc gcgcggaccg gctctttcag
     3181 atcatccaga tcctgcggcg gcggcgccgg ccggtgacgg cggcggcgat cgcggcggag
     3241 ctcgagcgct cgctccgcac cgtctaccgc gacatcgcgg atctcatggc gcaaagcgtg
     3301 ccgatccgcg gcgaggccgg catcggctat gtcctcgacc gcggcttcga catgccgccc
     3361 ttgatgctga ccgccgacga gatcgaggcg gcgatgctcg gcgcgcaatg ggtgatgcgc
     3421 cgtaccgatc ccga
//
LOCUS       DASYNS010000562         7391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3267538, whole genome shotgun sequence.
ACCESSION   DASYNS010000562 DASYNS010000000
VERSION     DASYNS010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7391
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3267538"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VGP50_17175"
     CDS             complement(<1..583)
                     /locus_tag="VGP50_17175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8029169.1"
                     /translation="MRKSGHWATGTVLGLLCLMYLITYVDRVNISTAAIVIKPELGLS
                     NADYGKVFSAFAWSYACFQIIGGWIGDRFGPRLTLGICGAIWASSTIATGLASGFWAL
                     IFARIVLGIGEGATFPTATRAMSNWTRASWRGFAQGITHSFARLGNAVAPPIVAALIA
                     FWSWRTSFVILGGLSLAWVVAWVLYFRDRPSDHR"
     gene            721..3591
                     /locus_tag="VGP50_17180"
     CDS             721..3591
                     /locus_tag="VGP50_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV8029170.1"
                     /translation="MEDEALAARLKRALRGDVLFDAFSRGRYSTDASIYQIEPRGVVV
                     PRDQDDVAAALALAREAGVPVLPRGGGTSQCGQTVGRALVIDCSKYMDAILSVDVERR
                     RALVQPGVVLDRLNKALKPKGLFFPVDVSTGDRATIGGMAGNNSCGARSLRYGNMVHN
                     VRAIDALFADGTAIRMGELPGNFEDAAAPERYRDLVRRLRALRRRESAEIAARIPKLL
                     RRVGGYNIDSIDDAGHNMAQLLVGSEGTLAFFNAIELELQAIPPHRVLGICHFPSFYQ
                     AMASAKRIVALGPTAVELVDRTMIELSRGIPLFRATVDRFVKGEPEALLLVEFAGENA
                     ATNRRGLEDLVELMGDLGLPGAVVEATEPAFQQAVWNVRKEGLNIMMSMKGDGKPISF
                     IEDCAVSLDDLADYTERLTAVFAKHGTYGTWYAHASVGCLHVRPVLNLKQELDVRKMR
                     AIAEEAFAIVREYKGSHSGEHGDGLVRSEFHEAMFGPRLSRAFEEVKDSFDPQGLMNP
                     GKIVRAPKMDDRSLFRFKPGYRTPPLDTALDWSEWGGFAGAVEMCNNNGACRKAEPGV
                     MCPSYRATADEQHLTRGRANSLRLALSGALGADALTSDAMAETMALCISCKGCKRECP
                     TGVDMARMKIEFLHQRRRRHGLSLRDRVVGYLPRYARAAARMAPLLELRDRIPVLARW
                     SEALLGFSARRPLPRWEREPYREPAPPSAAVRSVVLLVDTFTRWFEPENARAAARVLH
                     RAGYAVGGATPKGQRPLCCGRTFLAAGLVAEAKAEARRLLAALEHFVGLGVPIIGLEP
                     SCLLTLRDELPALLPGPASAALAKQARLFEEFVAAESAAGRWKLEIKPTPGKALLHGH
                     CHQKAFDTLGASVATLKLVPGLEVETFESTCCGMAGAFGYEAEHYDMSLSIGELGVLP
                     RMRAAAADTILVAAGTSCRQQIAHGAGRAALHPARVLERAMG"
     gene            complement(3581..4231)
                     /locus_tag="VGP50_17185"
     CDS             complement(3581..4231)
                     /locus_tag="VGP50_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV8029171.1"
                     /translation="MRHRFAYVVLILLAGAAIAIAAEAPQFTLRGDRFKPLTWETMTP
                     EQRTLAEHVLAGKRGSLNGPYNVLMRSPVMGDLAQKFGEYTRYDTSIPHPLNELAILV
                     TARFWSSQFEWSSHHKYGLEAGLSPALIDDLAAGKRPAQMSDDEAVVYDFCHELLYTK
                     AVSDTTFDATKEKFGERGIVDLIGVMGYYHVVAMALNVDRYPLPDNAKPELGPVTP"
     gene            4373..5806
                     /locus_tag="VGP50_17190"
     CDS             4373..5806
                     /locus_tag="VGP50_17190"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV8029172.1"
                     /translation="MCSDSDHHSGDTPRQDNETALREERRGFLKGALLTAAGGAVGLG
                     AASVALVTPAAAQARTNHYYVPATDKTVHWGYFSKTLKPVVEVESGDFVTIEALTHHA
                     NDDAERMVKGDPGLESVFRWDKTGKGVNRRGAGPMDASIFGRGAGEGFGVHILTGPVA
                     VRGAEPGDVLEVRIIDVKPRPTANPKYPGKAFGSNAAAWWGFHYHDLLTEPKPREVVT
                     IYEIDATGDRNWARALYNFRWTPQTDPSGVVHKIIDYPGVPVDHSTITENHGILQNVR
                     VPIRPHFGIIGLAPAEADFVDSIPPGYFGGNMDNWRIGKGATMYYPVAVPGGLLSVGD
                     SHASQGDSELCGTAIECSLTGVFQLVLHKNAALQGTSLADLKYPLLETQDEWVLHGFS
                     YANYLAELGEKAQSQIYEKSSLDGAMRDAFRKVRHFLMTTKGLSEDEAISLTSIAVDF
                     GVTQVVDGNWGVHAIIKKNIFAGAAEV"
     gene            complement(5856..6788)
                     /gene="ftsY"
                     /locus_tag="VGP50_17195"
     CDS             complement(5856..6788)
                     /gene="ftsY"
                     /locus_tag="VGP50_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV8029173.1"
                     /translation="MSAAPTGWLSRLKAGLTRSSTKLGEGISSIFARRRLDNETLAEL
                     EELLIAADLGIGLAEEVTGRLRRTRFNQEVAPEEIRAALAEEVAKSLSLVDRPLKLDP
                     ALKPMVILVVGVNGSGKTTTIGKLAKQYRDAGKTVVMAAGDTFRAAAVEQLKIWGERA
                     GATVIARETGADAAGLAFDAFEEARRRGADVLLIDTAGRLHNKTDLMAELQKIQRVLR
                     KIDPKLPHAVLLVLDATVGQNAHAQAETFRELIGVTGIVMTKLDGTARGGVLVSLAEK
                     YGIPVHYIGVGESADDLRPFTAEAFARSLVGLDA"
     gene            complement(6785..>7391)
                     /locus_tag="VGP50_17200"
     CDS             complement(6785..>7391)
                     /locus_tag="VGP50_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007439028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV8029174.1"
                     /translation="LERLRLSSLDPVEIDDALWRLIAEEPRLMPHLHLSLQAGDDLVL
                     KRIKRRHSRADALAACRRARDLRGDVALGADLIAGFPTESDAMFAETLSLVEEAALDY
                     LHVFPYSVRPGTPAARMPQVPHGLIRERAARLRAAGAAALERSLARRVGQTARVLVER
                     DGFGRSEHYAPVRFTGAIATGEIAALRIRAATPQALIGAVP"
BASE COUNT         1192 a         2562 c         2466 g         1171 t
ORIGIN      
        1 cgcgatggtc gctgggccgg tcgcggaaat aaaggaccca ggcgacgacc caggcgaggc
       61 tgaggccgcc caggatcacg aaggaggtgc gccaggacca gaacgcaatc agcgcggcga
      121 cgatcggcgg cgccaccgca ttgccgaggc gggcgaagga atgcgtgatc ccttgcgcga
      181 agccgcgcca actcgccctc gtccagttgg acatggcgcg ggtggcggtg ggaaacgtcg
      241 cgccttcgcc gatgcccagc acgatgcgcg cgaagatcag cgcccagaag cccgaagcga
      301 ggccggtcgc gatcgtcgat gacgcccaga tcgcgccgca gatgccgagc gtcagccgcg
      361 gtccgaaacg gtcgccgatc cagccgccga tgatctggaa gcaggcatag ctccaggcga
      421 aggcggagaa gaccttgccg taatcggcgt tgctcaagcc cagctccggc ttgatgacga
      481 tcgccgcggt cgagatgttg acgcggtcga cgtaggtgat gagatacatg aggcagagca
      541 ggcccagcac cgtgcctgtc gcccaatggc ccgatttgcg catttcgtcc cgatgtcggc
      601 gttgatgttt cgccgtctgg gccacagcct cgcatggcgg ccgtcgctgc ggcaagcggc
      661 ctcgccctgg aaatttgccc gcgcatcgcc tctaatctgc gccggaagcg gaggagaccc
      721 atggaagacg aagcgctggc ggcgcgattg aagcgcgcgc tgcggggcga cgtgcttttc
      781 gacgccttct cgcgcggccg ctacagcacc gatgcgtcga tctatcagat cgagccgcgc
      841 ggcgtcgtgg taccccgcga ccaggacgac gtcgccgcgg cgctcgcgct ggcccgtgaa
      901 gccggggttc cggtcctgcc gcgcggcggc ggcacctcgc aatgcggcca gaccgtcggc
      961 cgcgcgctgg tcatcgactg cagcaaatac atggacgcga tcctttccgt cgatgtcgag
     1021 cggcgccgcg ccctcgtgca gccgggcgtc gttctcgacc gcctcaacaa ggcgctcaaa
     1081 cccaagggcc tcttcttccc cgtcgacgtc tcgaccggcg atcgcgccac catcggcggc
     1141 atggccggca acaacagctg cggcgcgcgc agcctgcgct acggcaacat ggtgcacaat
     1201 gtgcgcgcca tcgacgcgct cttcgccgac ggcacggcga tccgcatggg cgagctgccc
     1261 ggcaatttcg aagacgcggc cgcgcccgag cgctatcgcg atctggtgcg gcggctgcgt
     1321 gcgctccgcc gccgggaatc cgccgagatc gccgcgcgca tcccgaagct gctgcgccgc
     1381 gtcggcggct acaacatcga cagcatcgac gatgccggcc acaacatggc gcagctcctg
     1441 gtgggatcgg agggcacgct cgccttcttc aacgcgatcg agttggagct tcaggcgatc
     1501 ccgcctcatc gcgttctcgg catctgtcat ttcccgagct tctatcaggc gatggcgtcg
     1561 gcgaagcgca tcgtcgccct cggtccgact gctgtcgagc tggtcgaccg caccatgatc
     1621 gagctgtcgc gcggcatccc gctcttccgt gccaccgtcg accgcttcgt caaaggcgag
     1681 cccgaagcgc tgctcctggt ggagttcgcg ggcgagaatg cggcgaccaa tcggcggggt
     1741 ctcgaggatc tcgtcgagct catgggcgat ctcggactcc ccggcgccgt cgtcgaggcg
     1801 accgagcccg ccttccagca ggccgtgtgg aacgtccgca aggaggggct caacatcatg
     1861 atgtcgatga agggcgacgg gaagccgatc tccttcatcg aggactgcgc cgtttcgctc
     1921 gacgacctcg ccgattacac ggaacgcctc accgcagtct tcgcgaagca cgggacctat
     1981 ggcacctggt atgcccatgc ctccgtcggc tgcctgcatg tgcggcccgt gctcaacctc
     2041 aagcaggagc tcgacgtccg gaagatgcgc gccatcgccg aggaggcctt cgcgatcgtg
     2101 cgcgaataca aaggttcgca ttcgggcgag catggcgacg gtctcgtgcg ctcggaattc
     2161 cacgaagcga tgttcgggcc ccggctctcg cgcgccttcg aggaagtgaa ggacagcttc
     2221 gatccgcaag ggctgatgaa cccggggaag atcgtgcgcg cgccgaaaat ggatgatcgc
     2281 tcgctcttcc gcttcaagcc tggctaccgc acgccgccgc tcgacacggc gctcgactgg
     2341 tcggaatggg gcggcttcgc cggcgccgtc gagatgtgca acaacaacgg cgcctgccgc
     2401 aaggccgagc ccggcgtcat gtgcccctcc tatcgcgcga cggccgacga gcagcatctg
     2461 acgcgcggcc gcgccaacag cttgcggctg gcgctgagcg gcgcgctcgg cgccgacgct
     2521 ctgacgtccg atgccatggc cgagaccatg gcgctgtgca tctcctgcaa gggatgcaag
     2581 cgcgaatgtc cgaccggcgt cgacatggcg cgcatgaaga tcgagtttct gcaccagcgg
     2641 cggcggcgcc acggcttgag cctgcgcgat cgtgtcgtcg gctatctgcc gcgctatgcg
     2701 cgagcggcgg cccgcatggc gccactcctc gaactgcgcg accgcattcc cgtactcgcg
     2761 cgctggagcg aggcgctcct cggcttcagc gcgcggcgcc cgctgccgcg ctgggagaga
     2821 gagccctatc gcgagcccgc gccgccttcc gccgcggtgc gcagcgtcgt cctgctggtg
     2881 gacaccttca cgcgctggtt cgagcccgag aacgcgcgcg ccgcagcccg cgtcctgcat
     2941 cgcgccggct atgccgtcgg cggcgccacg cccaaaggcc agcgcccgct ctgctgcggc
     3001 cgcacctttc tcgccgccgg cctcgttgcc gaggcgaaag cggaggcgcg ccgcctcctc
     3061 gcggcgctcg agcacttcgt cggcttgggc gtgccgatca tcggcctcga gccctcctgt
     3121 ctcctcacgc tgcgcgacga gcttccggcc ctcctgcccg gtcccgccag cgcggcgctc
     3181 gccaagcagg cgcggctctt cgaggaattc gtcgcggccg aaagcgccgc cggccgctgg
     3241 aagctcgaga tcaagccgac gccgggcaag gcgctgctgc acggccattg ccaccagaag
     3301 gctttcgaca cgctgggcgc gagcgtcgcc acgctgaagc tcgtccccgg cctcgaggtc
     3361 gagaccttcg agtccacgtg ctgcggcatg gccggtgcct tcggctacga ggccgagcat
     3421 tacgacatgt cgctcagcat cggcgagctc ggcgtcctgc cccgaatgcg cgccgctgcc
     3481 gcggatacga ttctcgttgc cgccggcacc agctgccgcc agcagatcgc ccatggcgca
     3541 ggccgcgccg cactccaccc cgcgcgcgtg ctcgagcgcg ctatggggtg actggtccga
     3601 gctcgggctt ggcgttgtcg gggagcgggt agcggtcgac gttcagcgcc atcgccacga
     3661 cgtggtagta gcccatcacg ccgatcagat cgacgatccc gcgctcgccg aatttctcct
     3721 tggtggcgtc gaaggtcgtg tcgctgaccg ctttcgtata gagcagctcg tggcagaaat
     3781 cgtagacgac ggcctcgtcg tccgacatct gcgccgggcg cttccccgcc gcgagatcgt
     3841 cgatgagcgc cgggctcaat cccgcctcga ggccgtattt gtggtgtgaa gaccattcga
     3901 attgcgagct ccagaagcgc gccgtgacga ggatcgcgag ctcgttgagc gggtgcggga
     3961 tcgatgtgtc gtagcgcgtg tactcgccga atttctgcgc gaggtcgccc atcaccgggc
     4021 tgcgcatcag cacattgtaa ggcccgttca gcgagccgcg cttgccggcg aggacgtgct
     4081 cggccaaggt ccgctgctcc ggcgtcatcg tctcccaggt gagcggcttg aagcggtcgc
     4141 cgcgcagcgt gaattgcggc gcttctgccg cgatcgcgat agcggcgccg gccagcagga
     4201 ttaaaacgac ataagcaaat ctgtgccgca tcgtgtttcc tcctccgaaa atccctctcc
     4261 ccttgtggga gggcaagggt cctccaccaa acgaacatag cacgcgcacg ctggcatgcc
     4321 gcttgcttcg tcgcgcctag cccacatttg tcggccagga ggggaggcgc atatgtgcag
     4381 cgacagcgat caccacagcg gcgacacgcc gcgacaggat aacgagacgg cccttcgcga
     4441 ggaacggcgg ggctttctca aaggcgccct gctgaccgcg gccgggggcg ccgtcggcct
     4501 cggcgctgcc agcgtcgcgc tggtcacgcc ggccgcggcg caggcgcgca ccaatcacta
     4561 ctatgtgccg gccaccgaca agacggtgca ctggggttat ttcagcaaga ccttgaagcc
     4621 ggtggtcgag gtcgagagcg gcgacttcgt caccatcgag gcgctgacgc atcacgccaa
     4681 tgacgatgcc gagcgcatgg tcaagggcga ccccggcctc gaaagcgtct ttcgctggga
     4741 caagaccggc aaaggcgtga accggcgcgg cgccggtccg atggacgcct cgatctttgg
     4801 ccgcggcgcc ggcgagggct tcggcgtgca catcctcacc ggccccgtcg cggtgcgcgg
     4861 cgccgagccc ggcgacgttc tcgaagtgcg catcatcgac gtgaagccgc gtcccaccgc
     4921 caacccgaaa tatccgggca aggccttcgg cagcaacgcc gccgcctggt ggggcttcca
     4981 ctaccacgat ctcctcaccg agcccaagcc gcgcgaggtg gtcaccatct acgagatcga
     5041 cgccacgggc gaccgcaact gggcgcgggc gctctacaat ttccgctgga cgccgcagac
     5101 cgacccgagc ggcgtcgtcc ataagatcat cgactacccg ggcgtcccgg tggaccacag
     5161 cacgatcacc gagaatcacg gcattctcca gaatgtgcgc gtgccgatcc ggccgcattt
     5221 cggcattatc ggcctggcgc cggcggaagc cgatttcgtc gattcgatcc cgcccggcta
     5281 tttcggcggc aatatggaca attggcggat cggcaagggc gccacgatgt attacccggt
     5341 cgccgtgccg ggcggcctgc tctccgtcgg cgacagccat gcctcgcaag gcgacagcga
     5401 gctttgcggc accgccatcg aatgctcgct caccggggtc ttccagctcg tgctgcacaa
     5461 gaacgcggca cttcagggca cttccttggc agatctcaaa tatccgctgc tcgagaccca
     5521 ggacgaatgg gtgctgcacg gcttcagcta tgcgaactat ctggccgagc tcggcgagaa
     5581 ggcgcagtcg cagatctacg agaagtcctc cctcgacggc gccatgcgcg acgccttccg
     5641 caaggtgcgg catttcctca tgacgaccaa agggctgagc gaggacgaag cgatctcgct
     5701 gacctcgatc gccgtcgatt tcggcgtgac gcaagtggtc gacggcaact ggggcgtcca
     5761 cgccatcatc aagaagaaca tcttcgccgg cgccgccgag gtgtgaccgg ttccgggcgc
     5821 tcacccctca ccaagggtga ggggtgccgc gtctcctacg cgtccaaccc cacgaggctc
     5881 cgcgcgaacg cctccgccgt gaagggacgg agatcgtcgg cgctttcgcc gacgccgatg
     5941 taatgcacag ggatgccgta tttctcggcg agcgacacaa gcacgccgcc gcgcgccgtg
     6001 ccgtcgagct tggtcatgac gatgccagtg acgccgatga gctcgcggaa ggtctcggcc
     6061 tgcgcatggg cgttctgtcc gacggtggca tcgagcacca gcagcaccgc atgcggcagc
     6121 ttgggatcga tcttccgcag cacgcgctga atcttctgca gctccgccat gaggtcggtc
     6181 ttgttgtgga gccgcccggc cgtgtcgatc agcagcacgt ccgcgccgcg ccggcgcgcc
     6241 tcttcgaaag cgtcgaaggc gaggcccgcg gcatccgctc ccgtctcgcg cgcgatgacg
     6301 gtggcgccgg cgcgctcgcc ccagatcttg agctgctcga ccgcggccgc acggaaggtg
     6361 tcgccggccg ccatgaccac ggtcttgccg gcatcccgat actgcttcgc gagcttgccg
     6421 atggtggtgg tcttgccgct gccattgacg cccaccacca ggatcaccat cggcttcaag
     6481 gcgggatcga gcttcaatgg ccggtccacc agcgacagcg acttcgccac ctcttcggcg
     6541 agcgcggcgc ggatctcctc gggcgccact tcctggttga agcgcgtgcg gcggagccgt
     6601 cccgtcactt cctcggcgag gccgatgccg agatcggcgg cgatgagcag ctcctcgagc
     6661 tcggccagcg tctcgttgtc gagccggcgc cgcgcgaaga tgctgctgat gccctcgccg
     6721 agcttcgtcg aagagcgcgt aagcccggcc ttgaggcggg aaagccagcc ggtcggcgcc
     6781 gcgctcatgg gacggcgcca atgagcgctt gcggcgtggc cgctctgatt cgcagggcgg
     6841 cgatttcgcc tgtcgcgatc gctccggtga aacgcacggg ggcataatgt tcgctccggc
     6901 cgaagccgtc gcgctcgacc agcacgcgcg ccgtctggcc gacgcggcgc gcgagcgacc
     6961 gctccagtgc cgccgcaccc gccgcgcgca gccgcgcggc gcgctcgcga atcaggccat
     7021 gcggcacctg cggcatgcgc gcggccggtg ttcccggccg gacgctgtaa gggaagacat
     7081 ggagatagtc gagcgccgcc tcctcgacga gcgagagcgt ctcagcgaac atcgcgtcgc
     7141 tttcggtggg aaagccggcg atgaggtcgg cgccgagcgc gacgtcgccg cgcaagtcgc
     7201 gggcgcgccg gcacgcagcc agcgcgtcgg cgcggctgtg gcggcgctta atgcgcttca
     7261 gcacgaggtc gtcgcccgcc tggagcgaga gatggagatg gggcatgagg cgcggctcct
     7321 cggcgatcaa acgccagagc gcgtcatcga tctccacggg gtcgagcgag gacagcctga
     7381 gccgctcgag c
//
LOCUS       DASYNS010000563         1672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_614371, whole genome shotgun sequence.
ACCESSION   DASYNS010000563 DASYNS010000000
VERSION     DASYNS010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614371"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VGP50_17205"
     CDS             complement(<1..950)
                     /locus_tag="VGP50_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029175.1"
                     /translation="MTAAICATSRASSRRSSRAISESLSVAGTAALSSPADSTTLLVN
                     SSTKSGTPSVLATIAATVSALQAVCGGDVGDQPRAFGAAQAIQRQDGCLGTRQPRRRE
                     VRPRRHQDEQPRLADAVGEARHQFERGGVDPVRVLQHQEDRITLAQMDDLVDKERDGR
                     RLPLRRRQRQLRRRRLGRDRQEVSQQRDRGFVRRGAPREYCPELVDAGTRRVGGRKSG
                     SPSEMLDDRIEHAVPVIGRALQVNPGVKLMGDIFLQRLDRARFADTSLADHADDLTLA
                     RAGETPALEHQPHFVRPADERQRTGADRGEAALDRRFAEHA"
     gene            892..>1672
                     /locus_tag="VGP50_17210"
     CDS             892..>1672
                     /locus_tag="VGP50_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029176.1"
                     /translation="MARLDRLDEAREVAQIAAVIGRQFAFSVLAAIAARDGAALDAAL
                     DKLVAAGIVLPEGRGLERGFSFKHALVRDAAYESLLLGRRREWHERIARALEQSFPGT
                     VANEPELLAYHFAEAGSAAPACDYRMRAGDRAVSRSAYQEAVAHFSAGLKLADALPDP
                     ADQTRRKLDFLLKLGPALMVTRGLQSAEAEDAFSRAAEIGDRQGDASALYKAKWGLWL
                     NANIRRKTALARDRARELVTLAQRSGDGDLLLEAYHCRWSTA"
BASE COUNT          256 a          559 c          582 g          275 t
ORIGIN      
        1 gcgtgctcgg cgaagcggcg gtcgagagcc gcttcgccgc gatccgcacc ggtacgctgc
       61 cgctcgtcgg ccgggcgcac gaaatggggc tgatgctcga gcgctggcgt ctcgcccgct
      121 cgggcgaggg tcagatcgtc agcgtggtcg gcgaggctgg tatcggcaaa tcgcgcgcga
      181 tcgaggcgtt gcaggaagat gtcgcccatg agcttcacac ccggattcac ctgcagtgct
      241 cgccctatca ccgggacagc atgctctatc cgatcatcca gcatctcgct cgggctgccg
      301 gatttgcgcc cgccgactcg gcgggtgccc gcatcgacaa gctccggaca atattcgcgc
      361 ggcgcgccgc gacggacgaa accgcggtcc cgttgctggc tgacctcctg tcgatcccgg
      421 ccgaggcgcc gccggcgcaa ttgtcgctga cgccggcgca acggaaggcg gcgaccatcg
      481 cgctccttgt cgacgagatc gtccatctgg gcgagagtga tccggtcctc ctggtgctgg
      541 aggacgcgca ctggatcgac cccaccacgc tcgaactgat gacgcgcctc gccgacagca
      601 tcagcgaggc gcggctgctc gtcctggtga cggcgcggcc ggacttcgcg gcgccttggc
      661 tgacgcgtcc ccaggcaacc gtcttgacgc tgaatcgcct gagccgcgcc gaatgcgcgc
      721 ggctggtcgc ccacgtcgcc gccgcacacg gcttgcaagg cggaaaccgt ggcggcgatc
      781 gtggccaaga ccgatggcgt cccactcttc gtcgaggaat tgaccaagag cgtggtggaa
      841 tcggcgggcg aggacagcgc ggcggtgccg gcgacgctca aggattcact gatggcccgg
      901 ctcgaccgcc tcgacgaggc gcgcgaggtg gcgcagatcg ccgcggtcat cggccggcaa
      961 ttcgccttct ctgtgcttgc ggccatcgcc gccagggacg gcgccgcgct cgatgcggcg
     1021 ctggacaagc tggtcgcggc gggcatcgtc cttcccgagg gacgcggcct ggaacgaggc
     1081 ttcagcttca agcacgcgct ggtgcgggac gccgcctatg aaagtctgct cttgggccgc
     1141 cgtcgcgagt ggcatgagcg cattgcgcgt gcgcttgaac agagcttccc cggaacggtg
     1201 gcgaacgagc cggagctcct cgcctatcat ttcgctgagg ccggatcggc tgctccggcc
     1261 tgcgactacc gcatgcgcgc cggtgaccgt gccgtgagcc gctcggccta tcaggaagcg
     1321 gtcgcgcatt tctcggccgg cctcaaattg gccgacgcac tgccggaccc cgccgatcag
     1381 acgcgccgca agctcgattt tttgctgaag ctcggcccag cgctcatggt gacgcgcggc
     1441 ctgcagagtg cggaggcaga ggacgccttc agccgtgcgg ctgagatcgg cgacaggcag
     1501 ggcgacgcta gcgccttgta caaggcgaag tggggtttgt ggctcaatgc caatatcagg
     1561 cgcaaaactg ctttggcgcg cgaccgagcg cgggagctgg tgaccctcgc gcaacgctcc
     1621 ggcgatggtg acctgctgtt ggaggcttac cattgccgct ggtcgacggc tt
//
LOCUS       DASYNS010000564         3400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_145880, whole genome shotgun sequence.
ACCESSION   DASYNS010000564 DASYNS010000000
VERSION     DASYNS010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145880"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VGP50_17215"
     CDS             complement(<1..511)
                     /locus_tag="VGP50_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEV8029177.1"
                     /translation="MQTTNEIRAAFLDYFAANGHEVVPSSPLVPRNDPTLLFTNAGMV
                     QFKNVFTGAEKRAYARAATAQKCVRAGGKHNDLENVGYTTRHGTFFEMLGNFSFGDYF
                     KDRAIELAWRLVTKEFGLDKERLLVTVFAEDDEAAALWRKIAGLPESRIIRIATSDNF
                     WTMGETGPCG"
     gene            714..2480
                     /locus_tag="VGP50_17220"
     CDS             714..2480
                     /locus_tag="VGP50_17220"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV8029178.1"
                     /translation="MNAEGRHRRFSLVLIKPSHYDDDGYVIQWLISPIPANALAILHG
                     LASDCAERRVLGDDVDLVVTAVDETNTRIDPERVARSIGPGGGLVGLVGVQSNEFPRA
                     MDIARRLRAAGIQVCIGGFHVSGCVAMLPELTPELQEAQRLGISLFAGEAEGRLDEVL
                     IDAQRGTLKPLYNFMADLPDIAGVPLPVLPAGIVKQTAGRTASFDAGRGCPFQCSFCT
                     IINVQGRKSRHRSADDVEAIVRRHLAQGINHFFITDDNFARNSDWERIFDRLIQLREG
                     EKLKIKFIIQVDTLCHRIPGFIEKAHRAGVTKTFIGLESINPESLLGAKKRQNKITEY
                     RKMLLAWKRVGITTYGGYILGFPSDTPETILRDIAIIQRELPLDLLEFFCLTPLPGSE
                     DHKKLYLAGAWLDPDLNKYDVEHVTTTHPLMSRAAWEGIYEQAWRAYYSPEHMERVLR
                     RAAATRGSFRSMAFQLLWFYGSITIERVHPLQGGYFRRKSRRDRRPSLPREGMLVFYP
                     RYALEIVAKHVRLAAMFLRLRRMQQRLAREPGLRDYMDQALTPVADHELDELELFTVS
                     AGAKSAVVKARKEEQARSRLAG"
     gene            2588..3289
                     /locus_tag="VGP50_17225"
     CDS             2588..3289
                     /locus_tag="VGP50_17225"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV8029179.1"
                     /translation="MGSSLEVADDLASAGVHAGGLVLALIFSLCLLRRAEDPVARFAI
                     RALGLAWLILYLASISYHLTHIETAWDPLTTALDDGAIFVAIAGTYTPVALLILPPAD
                     GRFAVRLLWCAGAAGAATGFVMILTGDLSWYQPSVLVICSIYGWGPAIAYCRTLRRAM
                     PRGAGFLILASGLVYVAGSYFYRDHRLPWHHTYWHMAVVIGCLLDFTAIAALLAATRR
                     RALAPARSQDSPVRR"
BASE COUNT          536 a         1141 c         1096 g          627 t
ORIGIN      
        1 ggccgcaggg acccgtctcg cccatcgtcc agaaattgtc cgaggtggcg atgcggatga
       61 tccggctttc cggcaagccg gcgatcttgc gccacagcgc cgccgcctcg tcgtcctcgg
      121 caaagaccgt gaccagcagc cgctccttgt cgaggccgaa ctccttggtc accagccgcc
      181 aagccagctc gatggcgcgg tccttgaaat aatcgccgaa ggagaaattg ccgagcatct
      241 cgaagaaggt gccgtgtcgg gtggtgtagc cgacattctc gaggtcgttg tgcttgccgc
      301 cggcgcgcac gcatttctgc gccgtggcgg cgcgggcgta ggcgcgcttc tcggcgccgg
      361 tgaagacgtt tttgaactgc accatcccgg cattggtaaa gagcagcgtc gggtcgttgc
      421 gcggcacgag cgggctcgac ggcaccactt cgtggccgtt cgcggcgaag tagtccagga
      481 aggcggcgcg aatttcgttc gtggtctgca tagtgcttat ctgggccctc cttggggcct
      541 ggccaagcgg cgggcctgct tctagcccgc gcggtcgcgg actgtccagc cgcgggcgcc
      601 aagggatcgc ctggcgccgc tccctcgacg cttcggtagt caatcttctt cggccgagcg
      661 accgatggtg gtatcgtgcc ctgcggctac aatcacgcac ataggggtcg gcgatgaatg
      721 ctgaggggcg acaccggcgc ttctcgctgg tcctgatcaa gccgtcgcat tacgacgatg
      781 acggctatgt catccagtgg ctgatctcgc ccataccggc gaatgccctg gcgattcttc
      841 acgggctcgc gagcgactgc gccgagcgtc gtgtcctggg cgatgacgtc gacctcgtcg
      901 tcaccgccgt cgatgagacc aatacacgca tcgaccccga gcgggtcgcg cgcagcatcg
      961 ggcccggcgg cggcttggtc ggcttggtcg gcgtccagtc gaacgagttt ccccgcgcca
     1021 tggacatcgc gcgccgactg cgcgcggcgg gtatccaggt atgcatcggc ggcttccacg
     1081 tgtcgggatg cgtcgccatg ctgccggagc tgacgcccga gctccaggag gcgcagcgtc
     1141 tcggcatctc gctcttcgcc ggcgaggcgg agggccgcct cgatgaagtg ctcatcgacg
     1201 cgcagcgggg cacgttgaag ccgctctaca acttcatggc cgacctgccc gacatcgccg
     1261 gcgtgccatt gccggtgctt cccgccggca tcgtcaagca gacggcgggg cggacggcga
     1321 gcttcgatgc cggccgcggc tgtcccttcc aatgctcgtt ctgcaccatc atcaatgtgc
     1381 agggacggaa atcgcgccac cgctcggcgg acgacgtcga agcgatcgtc cggcgccacc
     1441 tggcgcaggg catcaaccac ttcttcatca ccgacgataa tttcgcgcgc aacagcgact
     1501 gggagcgcat tttcgaccgg ctgattcagc tgcgcgaggg cgagaagctg aagatcaaat
     1561 tcatcatcca ggtcgatacg ctctgccacc gcattcccgg attcatcgag aaggcgcacc
     1621 gcgccggcgt caccaagacg ttcatcggcc tcgagagcat caatccggaa agcctgctcg
     1681 gcgccaagaa acggcagaac aagatcaccg aataccgcaa gatgctgctt gcctggaagc
     1741 gcgtcggcat caccacttac ggcggctaca tcctgggctt cccgagcgat acgccggaaa
     1801 ccattctgcg cgacatcgcc atcatccagc gcgagctgcc gctcgacctg ctcgagttct
     1861 tctgcctgac gccgctgccg ggctcggagg accacaagaa gctctatctc gccggcgcct
     1921 ggctcgatcc cgacctcaac aaatacgacg tcgagcacgt gacgaccacg catccgctga
     1981 tgtcgcgggc ggcgtgggag ggcatctatg agcaggcttg gcgcgcctat tactcgcccg
     2041 agcacatgga gcgcgtgctg cggcgcgccg cggcgacgcg cggcagcttc cgcagcatgg
     2101 cgttccagct cctgtggttc tacggctcca tcaccatcga gcgcgtccat ccgcttcaag
     2161 gcggctattt ccgccgcaag tcgcgccgcg accgccgccc gtcgctgccg cgcgagggca
     2221 tgctcgtctt ctatccgcgc tatgcgctcg agatcgtcgc gaagcacgtc cggctggccg
     2281 cgatgttttt gcgtctccgg cggatgcagc agcggctcgc gcgcgagccc ggcttgcgcg
     2341 actacatgga ccaggcgctg acgccggtgg ccgatcacga gctcgacgag ctcgagctct
     2401 tcacggtgag cgccggcgcc aagtcggccg tcgtcaaggc ccgcaaggag gagcaggcgc
     2461 ggtcccgtct cgccggttga aggtggggct tcaaaagccc cgaaagtgat cccatattca
     2521 tagaacagag gttgagtggg gtggttagcc tgacgtcgcc gcgtgccggc gagctggagc
     2581 gcgccccatg ggttcgtctc tcgaggtcgc ggatgatctg gcgagtgccg gggtccatgc
     2641 cggtggcttg gttctggcgc tgatcttcag cctctgtctc ctgcgtcgcg ccgaggaccc
     2701 ggttgcccgc ttcgccatcc gcgcgttggg cctcgcctgg ctcatccttt atctcgcctc
     2761 gatcagctat cacctgacgc atatcgagac ggcgtgggat ccgttgacca ccgcgctcga
     2821 cgacggcgcg atcttcgtgg cgatcgccgg cacctacacg ccggtcgcgc tcttgatcct
     2881 gccgccggcc gatggccgct tcgccgtgcg tctcttgtgg tgcgccggcg cggcgggcgc
     2941 tgccaccggc ttcgtcatga tcctgaccgg cgatctctcc tggtatcagc cgagcgtgct
     3001 ggtgatctgc tcgatctatg gctggggccc ggcgatcgcc tattgccgga cgttgcggcg
     3061 cgccatgccg cgcggcgccg gtttcctgat cctggcgagc ggcctcgtct acgtcgccgg
     3121 ctcctatttc taccgcgacc accgcctgcc ctggcatcac acttattggc acatggcggt
     3181 cgtgatcggc tgcctcctcg acttcacggc catcgccgcc ttgctcgccg cgacccgccg
     3241 gcgcgcattg gcaccggcga gaagtcaaga ttcaccagtg aggcggtgag acagtgagga
     3301 agatttaacc acagaggcgc agaggcgcag agaagagggt ttggcgcgcg ctgcgcctca
     3361 ccggagaatc ttctttcttc gatgcgcacc ccggaaaagg
//
LOCUS       DASYNS010000565         3265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_456175, whole genome shotgun sequence.
ACCESSION   DASYNS010000565 DASYNS010000000
VERSION     DASYNS010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456175"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="VGP50_17230"
     CDS             complement(<1..426)
                     /locus_tag="VGP50_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEV8029180.1"
                     /translation="MRATGVISAAAITGAVLSTMTIAPSNAQPVDPAILIGDSDFGGV
                     VTGPSGPEAGVWVIAETSDLATKFAKIVVTDDRGRYVIPDLPKATYSLWVRGYGLVDS
                     PKRQSAPGKLVDLTAVPAPSAAAAAEYYPGMYWYAMLKIP"
     gene            680..1540
                     /locus_tag="VGP50_17235"
     CDS             680..1540
                     /locus_tag="VGP50_17235"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV8029181.1"
                     /translation="MTFDVPAGACDCHVHVIPDPARFPFAPERVYTPPLATADELLAL
                     QRLLHLDRVVIVHPSIYGTDNSATLDGMRRLGRARARGVAVIDERISAAALDDMASAG
                     MVGLRVNLETVGEVDPRASARKLQAAAELAKDRLWHLQVYTRLPVIAALAEQLRGLPV
                     PLVIDHFGGARAELGLQQPGFDTLLALLRSGNTYVKISGCYRSSSATPDYADTAPLAQ
                     ALIAANPERVVWGTDWPHPDAGRGRPRDVTPYYPIDDGNVLNQLARWAPDPAVRKKIL
                     VDNPARLYGF"
     gene            1617..2819
                     /locus_tag="VGP50_17240"
     CDS             1617..2819
                     /locus_tag="VGP50_17240"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV8029182.1"
                     /translation="MREAKGNRPAPPSYDALRTRAEALVPVLRERAAKTEALRRVPEE
                     TIADLHASGLFRILQPARYGGSELPYGAFVELASIVGRGCGSTAWVLNNLASHHWMLA
                     YWPKEAQDEIWGASPDTLIASAFVFPGGRARRVPGGFRLTGRWPFSSGVDASSWNMIA
                     AVVPDEATGASEYRVFLLPASDYAVVDTWYVSGLAGTGSKDVVANDVFVPEYRSLSTE
                     AGKGGPSPGSAVNPAPIFRLPWFALFGILIASPALGIAKGAVEHYVGATRTRLGTYSS
                     KSLADFQTIQLHVAEAAALVDAAEALLLKDCAEAMRFAEADRVPPIEDKVRWRRDGAH
                     ASRLCTAAVDVIFAAAGGGAIYESNPLQRAWRDIHAASAHFAVNWDANGTIYGRVALG
                     LPADTPNL"
     gene            2850..>3265
                     /locus_tag="VGP50_17245"
     CDS             2850..>3265
                     /locus_tag="VGP50_17245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV8029183.1"
                     /translation="MPETPSEQPTAPPFDVRDFRSALGSFATGVTICTTRGREGEPVG
                     LTVNSFASVSLDPPLVLWSQSLYAQSLQAFQEATHFVVNVLAADQIALATRFAGDHDN
                     RFLDVEHIVPEHGAPVIAGCAAHFECRNEARYYGGD"
BASE COUNT          477 a         1178 c         1097 g          513 t
ORIGIN      
        1 ggggatcttg agcatcgcgt accagtacat gcccggatag tattccgccg ccgcggcggc
       61 gctgggcgcc ggcaccgccg tcaagtcgac gagctttccg ggcgcgctct gccgtttcgg
      121 cgaatccacc agcccgtagc cacggaccca caggctgtag gtcgccttgg gaaggtcggg
      181 gatgacgtag cggccgcgat cgtcggtcac gacgatcttg gcgaacttgg tggcaagatc
      241 gctcgtctcg gcgatgaccc agacgcccgc ctccggcccg ctcggaccgg tgacgacgcc
      301 gccaaagtcg ctgtcgccga tgaggatggc gggatccacc ggctgtgcgt tcgatggggc
      361 gatcgtcatg gtcgagagta cggcgccggt gatcgctgcg gccgagatga cgcccgttgc
      421 cctcatggaa gtcctccctt ggacgtgtcc cgcgcggctg cttggccgag ccggctgcgg
      481 tgtcgtgcga aagcgtggcg catcgcgcgg caccctcgca agacaaaagg gcgatcgcgg
      541 gcggggaccg ccgatggacg gggcgcgtcg cgaaccctac catgagcgcc gcatatccct
      601 ctccccttgc ggggagaggg ataccatgcc ctcgacagga gaaatcgtga ctccaccagc
      661 gccgatgccg aggacgcccg tgactttcga cgtgcccgcg ggggcctgcg actgccatgt
      721 ccatgtgatc cccgatccgg cgcggtttcc cttcgcgccc gagcgcgtct atacgccgcc
      781 gctcgcaacc gcggacgagc tgctcgcgct ccagcgcctc cttcatctcg accgcgtcgt
      841 catcgtccac ccgagcatct acggcaccga caacagcgcg acgctcgacg ggatgcgccg
      901 cctcggccgc gcccgggcgc gcggcgtcgc cgtgatcgac gagcgcatat ccgccgccgc
      961 gctcgacgac atggcaagcg cggggatggt cggcctccgc gtcaatctcg agaccgtcgg
     1021 cgaggtcgat cccagggcgt cggcgcggaa gcttcaggcg gcggccgagc ttgcgaagga
     1081 ccgcttgtgg catctccagg tctatacgcg gctgcccgtc atcgccgcgc tggcggagca
     1141 gctccgcggc ctgccggtgc cgctggtcat cgaccatttc ggcggcgccc gcgccgagct
     1201 tggactgcag cagccgggct tcgacacgct cctcgccttg ctgcgctcgg gcaacaccta
     1261 tgtgaagatc tccggctgct accgcagctc gtcggccacg ccggactacg ccgacacggc
     1321 gccgctcgcg caagcgctca tcgccgccaa cccggagcgc gtcgtctggg gcaccgactg
     1381 gccgcatccc gatgccgggc gcggccggcc gcgcgacgtc acgccctatt atccgatcga
     1441 cgacggcaac gtgctcaatc agctcgcgcg ctgggcgccg gacccggcgg tccgcaagaa
     1501 gatcctggtc gacaacccgg cgcggctcta tggattctga gcgccatttg acagaaagcg
     1561 gtgtccgtgt gttatttatc tgttgataaa ttgatcggac gagaggctcg ccgctgatgc
     1621 gcgaagcgaa aggcaaccgg ccggcgccac cctcctatga cgcgctacgc acgcgcgccg
     1681 aggcgctggt gccggtgctg cgcgagcgcg ccgccaagac cgaggcgctc cgccgcgtgc
     1741 ccgaggagac catcgccgat cttcacgctt ccggcctttt ccgcatcctg cagccggcgc
     1801 gctatggcgg cagcgagctg ccctatggcg ccttcgtcga gctggccagc atcgtcggcc
     1861 gcggctgcgg ctcgaccgcc tgggtgctga acaatctcgc gagccatcac tggatgctgg
     1921 cctactggcc gaaggaggcg caggacgaga tctggggcgc atcgcccgac acgctcatcg
     1981 cctcggcctt cgtgttcccc ggcggccgcg cgcgccgcgt tccgggcggc ttccgcctca
     2041 ccggacggtg gcccttctcg agcggtgtcg acgcctcgtc ctggaacatg atcgcggccg
     2101 tggtgcccga cgaagccacg ggcgccagcg agtaccgcgt gttcctgctg ccggcgagcg
     2161 actatgccgt ggtcgacacc tggtatgtgt cggggcttgc cggcaccggc agcaaggacg
     2221 tcgtcgccaa cgacgtgttc gtgccggagt atcgcagctt gtcgaccgag gcgggcaagg
     2281 gcgggccgtc gccgggcagc gccgtcaatc cggcgccgat cttccgcctg ccctggttcg
     2341 cgctcttcgg catcctcatc gccagccccg ctctcggcat cgccaagggc gcggtcgagc
     2401 actatgtcgg cgcgacgcgc acgcggctcg gcacctacag cagcaagagc ctcgccgatt
     2461 tccagaccat ccagctccac gtcgcggagg ccgcggccct cgtcgacgcc gcggaggcgc
     2521 tgctgctcaa ggattgcgcc gaggccatgc gctttgccga ggccgaccgc gtgccgccca
     2581 tcgaggacaa agtgcgctgg cgccgcgacg gcgcccatgc gtcccggctc tgcacggcag
     2641 cggtcgacgt catcttcgcg gcggcgggcg gcggcgccat ctacgagagc aatccgctcc
     2701 agcgggcgtg gcgcgacatc cacgcggcga gcgcgcattt cgcggtcaat tgggacgcca
     2761 acggcacgat ctatggccgt gtcgctcttg ggctccccgc ggacacgccc aacttgtagc
     2821 cggccatgac cgcgagcaaa gatcgagcga tgccggagac gccgtcggag cagccgacag
     2881 cgccgccttt cgacgtgcgc gacttccgca gcgcgctcgg ctccttcgcg acgggcgtca
     2941 ccatctgcac gacgcgcggg cgcgagggcg agccggtcgg cctcaccgtc aattccttcg
     3001 cctcggtgtc gctcgatccg ccgctcgtgc tgtggagcca gtcgctctat gcgcagagcc
     3061 tgcaggcctt ccaggaggcg acgcatttcg tggtcaacgt gctggccgcc gaccagatcg
     3121 cgctcgccac gcgctttgcc ggcgaccacg acaaccgctt cctcgacgtc gagcacatcg
     3181 tgccggagca tggcgcgccg gtgatcgccg gctgcgccgc gcatttcgaa tgccgcaacg
     3241 aggcgcgcta ctacggcggc gacca
//
LOCUS       DASYNS010000566         1899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3424049, whole genome shotgun sequence.
ACCESSION   DASYNS010000566 DASYNS010000000
VERSION     DASYNS010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3424049"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..>1899
                     /locus_tag="VGP50_17250"
     CDS             353..>1899
                     /locus_tag="VGP50_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEV8029184.1"
                     /translation="MDFEKYTERSKGFVQAAQTLAQRQGNQQITPDHLLKVLLDDKEG
                     LASNLIRAAQGDPNEARTAVEAALQKLPRVEGSGAGQVYLSPEMGRLFAEAEKVAEKA
                     GDSFVTVERLLLAMMLLKDTPAAKALAAAGITPQSLNRAIEDVRKGRRADSASAEEGY
                     DALKKYARDLTAVARDGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLALRIVHGDVPESLKDKRLLSLDLGALVAGAKFRGEFEERLKAVLQEITAAQGEII
                     VFIDELHTLVGAGKAEGAMDASNMLKPALARGELHCVGATTLDEYRKHIEKDAALARR
                     FQPVFVNEPTVEDTISILRGIKEKYELHHGVRITDGAIVAAATLSNRYITDRFLPDKA
                     IDLMDEAAARLRMAAESKPEAIDELDRRIMQLKIEREALKKESDDASRERLERLEDEL
                     EDLEKKSAELTAQWRAEKDKLAGAQKLKEQLDQARIELEQVQRRGDWARAGELTYGVI
                     PELERKL"
BASE COUNT          365 a          623 c          661 g          250 t
ORIGIN      
        1 cgatgacgcc agcggacacg gagatgaccg gccccgagtc cgagtccgag ggcgatgctg
       61 atccaacgcc gtcttcgtcg acgccgccgg gcgagcccga ggtcccgccg accgcctgat
      121 cggcgccgcc tgtcgcaagc ggcggcgaga aaggccgaag tcgatgcacc ggcacggtct
      181 cgtcgtgccg tatgtgggcg tcttctcgcg ataggggcga aaggacggtc gcgtaattcg
      241 cgcggggctg cccttgagcc ggcgctagcg cgcgccaata tccaaggcgt ggagggcggg
      301 ctgtcgttgc ctcggcagag ggacgggcgc gccggttcat cgcggggaca tcatggactt
      361 cgaaaaatac acggagcggt cgaagggctt cgtccaggcc gctcagacct tggcacagcg
      421 ccagggcaac cagcagatca cgcccgacca tcttctgaag gtgctgctcg acgacaagga
      481 agggcttgcg tcgaacctca ttcgcgcggc acagggcgat cctaacgagg cgcgcaccgc
      541 cgtcgaggcg gcgctgcaga agctgccgcg cgtcgaaggc agcggcgccg ggcaggtcta
      601 tctttcgccc gagatgggtc gcctcttcgc cgaggcggag aaggtggccg agaaggccgg
      661 cgacagcttc gtgacggtcg agcggctgct gctggcgatg atgctgctca aggacacgcc
      721 ggcggcgaag gcgctcgccg ccgcgggcat cacgccgcaa agcctcaacc gcgccatcga
      781 ggacgtccgc aaaggccgcc gcgccgacag cgcgagcgcc gaggaaggct atgacgccct
      841 caagaaatac gcacgcgacc tcacggcggt ggcccgcgac ggcaagctcg atccggtgat
      901 cggccgcgac gaagagattc gccgcaccat ccaggtgctg tcgcggcgca ccaagaacaa
      961 cccggtgctg atcggcgagc ccggcgtcgg caagaccgcc atcgtcgaag gcttggcgct
     1021 gcgcatcgtc catggcgacg tgcccgagag cctcaaggac aagcgcctct tgtcgctcga
     1081 tctgggggcg ctcgtcgctg gcgccaagtt ccgcggcgag ttcgaggagc ggctcaaggc
     1141 cgtgctgcag gagatcaccg ccgcgcaagg cgagatcatc gtcttcatcg acgaactgca
     1201 cacgctcgtg ggtgccggca aggccgaagg cgcgatggac gcctccaaca tgctgaagcc
     1261 ggcgctggcg cggggcgaat tgcactgcgt cggcgccacg acgctcgacg agtatcggaa
     1321 gcacatcgag aaggatgcgg cgctggcgcg gcgcttccag ccggtcttcg tcaacgagcc
     1381 cacggtcgaa gacacgatct cgatccttcg cggcatcaag gagaaatacg agctccatca
     1441 cggtgtgcgc atcaccgacg gcgctatcgt cgccgcggcg acgctgtcga accgctacat
     1501 caccgaccgc ttccttccag acaaggccat cgacctcatg gacgaggctg cggcgcggct
     1561 gcgcatggcg gccgagtcga agcccgaggc gatcgacgaa ctcgaccgcc gcatcatgca
     1621 gctcaagatc gagcgcgagg ccctaaagaa ggagagcgac gacgcgtcgc gcgagcggct
     1681 cgaacggctt gaagacgagc tcgaagacct cgaaaagaag tcggccgagc tcaccgcgca
     1741 gtggcgcgcc gagaaggaca agctcgccgg cgcgcagaag ctcaaggagc agctcgacca
     1801 ggcgcggatc gagctcgagc aggtgcagcg gcgcggcgac tgggcgcgtg ccggcgagct
     1861 cacttacggc gtgatccccg agctcgagcg caagctcaa
//
LOCUS       DASYNS010000567         3606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3733957, whole genome shotgun sequence.
ACCESSION   DASYNS010000567 DASYNS010000000
VERSION     DASYNS010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3733957"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /locus_tag="VGP50_17255"
     CDS             <1..1564
                     /locus_tag="VGP50_17255"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEV8029185.1"
                     /translation="AVDLFDAVLDGRIEALWIMGTNPAVSMPRAGRVRAALEACPLVI
                     VSDCWPTDTTKLARIVLPAAGWGEKDGTVTNSERRISRQRAFRAPPGAAQPDWWMVTE
                     VARRMGWAEAFPYRGPAAIFREHARLSAFENDGRRVFDIGALARLSDDAFDRMEPVRW
                     PLPSGASGTDDGRLFHDGGFSTPDRHARFVPTPLRPLAEERCERWPLLLNTGRIRDQW
                     HSMTRTGRVPRLMQHVGEPLLEIHPADAARFGLADDGLAEIESRYGAATLRVRFACEQ
                     RPGEVFVPMHWTDGFSSAGPADRLVGAAVDPVSGQPELKATPVKVAARSMSWRGLLLR
                     RSETPVPGDLHWSRTPLASGHAYRLAGFEPLPGTVASEAWITGLLGAPAAAELVIYAD
                     PRRKVFRYASIVDDRLDACLFLAGPTGLLPGSDNLAPLLGTEIGPAARAGLVAGALPS
                     AGAAPDRGRTVCACFGVGLLTLHKAIREQCLTDVTEIGAALRAGTNCGSCIPELKEIL
                     REAGADGVGAAA"
     gene            complement(1654..2958)
                     /locus_tag="VGP50_17260"
     CDS             complement(1654..2958)
                     /locus_tag="VGP50_17260"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV8029186.1"
                     /translation="MAGLWVFSSAADATTFCYEPGAHGFGQTAVPSCVHGDTDVTRAQ
                     FQRSVPVANQSVTVLTTRDGTVQISLPPTWVQRYERAKERPDSSILAADARSHAYLLV
                     LFANKSDGYGLKSWSDQMRESLTNNYGKVNVSDTSKIVVNGRPALRFLLTGYVNGGSA
                     RFVVTTVEFETRIVSVAVWASPRVFTQRWDEFYALAAAVSESGAVAQKPQPPAPGEKP
                     RTVLVTGTGFVLSEQGQILTNNHVVNDCVDQVYGALPGKPELKLRLVARDPRNDLALL
                     MAPSRFSKAVALRGASIRPGDAVIAIGYPLFGTLSSEPIVTTGIVSALGGLQDDSRFL
                     QISASVQPGNSGGPLLDMAGKVVGVVTQKINQLVVARETGVLPENVNFALKTGVIRDF
                     LDKSAVSYQIANAGAAELAVADVAARARDYTLLIWCKAVLPE"
     gene            complement(3066..>3606)
                     /locus_tag="VGP50_17265"
     CDS             complement(3066..>3606)
                     /locus_tag="VGP50_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEV8029187.1"
                     /translation="RLDLSPLVPFGNWDELRGLREGARERGIEGLMLKRRDSAYMPGR
                     PKGPWFKWKRGALTVDTVLMYAQRGSGKRSSFYSDYTFGAWREGALVPVGKAYSGFTD
                     EELKRLDRWVRDNTIERYGPVREVAPKLVLEIAFDSVHRSSRHKSGLAMRFPRIHRIR
                     WDKPAAEADTVETLEKLVS"
BASE COUNT          582 a         1219 c         1186 g          619 t
ORIGIN      
        1 ggccgtcgat ctcttcgacg ccgtgctcga cggccgcatc gaggcgctct ggatcatggg
       61 caccaatccc gccgtcagca tgccccgcgc cgggcgcgtt cgtgcggcgc tcgaggcttg
      121 cccgctggtc atcgtctccg attgctggcc gaccgacacg acgaagcttg cccgcatcgt
      181 gctgccggcg gccggctggg gcgaaaagga cggcaccgtc accaattccg agcggcgcat
      241 ctcgcgccag cgcgcgtttc gtgcgccgcc cggcgcggcg cagcctgatt ggtggatggt
      301 gacggaagtc gcgcgccgca tgggctgggc cgaggccttc ccgtatcgcg ggccggccgc
      361 catctttcgc gagcacgccc gtctctccgc tttcgagaat gacgggcgtc gcgtcttcga
      421 catcggcgct cttgcccggt tgagtgacga cgccttcgac cggatggagc ccgtgcgctg
      481 gccgctgccg agcggcgcgt ccggcacgga cgacggccgc ctcttccatg acggcggctt
      541 ttcgacgccc gatcgtcatg cgcgattcgt gccgacgccg ttgcgcccgc tcgccgagga
      601 gcgctgcgag cgctggccgc ttctgctcaa taccgggcgc atccgcgacc aatggcacag
      661 tatgacgcgg acggggcggg tcccgcgcct catgcagcat gtcggcgagc cgttgctcga
      721 gatccacccc gccgatgccg cccgcttcgg tctcgccgat gacggtctgg cggagatcga
      781 gagtcgctac ggcgcggcga cgcttcgcgt gcgcttcgcg tgcgagcagc ggccgggcga
      841 ggtcttcgtg ccgatgcact ggaccgacgg cttcagctcc gccgggccgg cggaccgtct
      901 ggtcggcgcg gccgtggatc ccgtctcggg gcagcccgag ctcaaggcca cacccgtcaa
      961 ggtggcggcg cggtcgatgt cgtggcgtgg gctgctgctg cggcgctccg aaacgccggt
     1021 gcccggcgat ctccattggt cgcgcacgcc gctcgcctcg ggccatgcct accggctcgc
     1081 gggcttcgag ccgttgcccg gcaccgtcgc ttcggaagcc tggataaccg ggttgctggg
     1141 cgcgccggca gcggccgagc tcgtgatcta tgcggatccg cgccgcaagg tcttccgcta
     1201 tgccagcatc gtcgatgatc gtctcgatgc gtgcctgttc ctcgcgggtc cgaccggcct
     1261 tcttcccggg agcgacaacc tcgcacccct gctcggaacg gagatcggcc cggccgcgcg
     1321 cgcgggtctc gtggccggcg ccctgccgag cgccggcgcc gcgcccgatc gcggccgcac
     1381 cgtctgcgcc tgcttcggcg tcggcctcct gacgctgcac aaggcgatcc gcgagcagtg
     1441 cctgaccgac gtcacggaga tcggcgcggc gctgcgcgcc ggcacgaatt gcggctcctg
     1501 catccctgaa ctcaaggaga tcctgcgcga agccggcgcc gacggcgtcg gcgccgccgc
     1561 gtgacctccg gccgggcagc actctagaca ggctccggac gatgctcaga tacgatattg
     1621 ttgtcgctgg aatcagtcag cgagaatgca ggcttactcg ggcagtaccg ctttacacca
     1681 gatcagcagc gtgtagtccc tggcgcgagc ggcgacatcg gcgaccgcca gttcagcggc
     1741 gccggcgttc gcgatctgat aggacacggc gctcttgtcc aggaaatccc ggatcacgcc
     1801 cgtcttgagc gcgaagttta cgttttccgg caagacgccc gtctcgcgtg cgacgacgag
     1861 ctgattgatc ttctgcgtga cgacaccgac gaccttgccg gccatgtcca atagcgggcc
     1921 gccgctattg ccgggctgga cgctggcgct gatctgtaga aagcggctgt catcctgaag
     1981 gccgccaagc gcgcttacga tgcccgtggt cacgataggc tcggacgaaa gcgtgccgaa
     2041 aagaggatat ccgatggcga tgaccgcgtc gccgggacga atgctggcgc cgcgcagcgc
     2101 caccgccttg ctaaagcgcg acggcgccat aaggagcgcg agatcgtttc tcggatcgcg
     2161 ggcgaccagg cgcagcttga gctcaggctt tccgggcagg gcaccgtaga cctggtccac
     2221 gcaatcattc accacgtgat tgttggtgag gatctggcct tgctcggaga gcacgaagcc
     2281 ggtgccggtc acaagcactg tacgtggctt ctcgcccggt gctggtggct gcggcttttg
     2341 cgcgaccgcg ccggactcgc tcacagccgc ggcgagcgcg taaaactcgt cccatcgttg
     2401 agtgaagacc ctggggctcg cccaaaccgc aaccgagacg atccgcgttt cgaattcgac
     2461 ggtcgtcacc acaaatcgag cggaaccacc attcacgtat ccggtcaaca gaaagcgaag
     2521 agcggggcgg ccattgacga cgatcttgga agtgtcggaa acattgacct tcccgtaatt
     2581 gttcgtgagg ctctctcgca tctggtccga ccagcttttc aagccataac catccgactt
     2641 gttcgcaaaa aggacgagca aataggcatg tgaacgggca tcggcggcta gaatcgaact
     2701 gtccggccgc tcttttgccc gctcgtatcg ctgcacccat gtcggcggca gcgaaatctg
     2761 cacagtaccg tcgcgcgttg tgagaacggt cacgctctgg ttggcgaccg gaaccgaccg
     2821 ctggaattga gccctggtga cgtcggtgtc accgtgaaca caagaaggca ccgccgtctg
     2881 gccgaagccg tgcgctcccg gttcgtaaca aaaggtcgtg gcgtcggcag ctgacgaaaa
     2941 tacccagagg cccgccaaga ctgtcaaaag ccgtcgcatg aatgagctgc cccaaccaga
     3001 ccgtcaccgg atttagctta gcatatcgct gagaccatcg gccatgggga tgggatggcg
     3061 caaagctagc tcaccagctt ctccagcgtc tcgaccgtgt cggcttcggc cgctggcttg
     3121 tcccagcgga tgcggtggat gcgcgggaag cgcatggcca atccggattt gtggcggctc
     3181 gagcgatgga cgctgtcgaa ggcgatctcg agcaccagct tcggcgccac ctcgcgcacg
     3241 gggccgtagc gctcgatcgt gttgtcgcgc acccagcggt cgaggcgctt cagctcctcg
     3301 tcggtgaagc ccgaataggc cttgcccacc ggcaccagcg cgccctcgcg ccaggcgccg
     3361 aacgtgtaat cggagtagaa ggacgagcgc ttgcccgagc cgcgctgcgc atacatgagg
     3421 acggtgtcga cggtgagcgc gccgcgcttc catttgaacc agggcccttt cggccggccc
     3481 ggcatataag cggaatcgcg gcgcttcagc atgagtccct cgatgccgcg ctcgcgcgct
     3541 ccctcgcgca atcccctgag ctcgtcccag ttcccaaacg gcaccagcgg cgagagatcg
     3601 aggcgc
//
LOCUS       DASYNS010000568         3760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3889204, whole genome shotgun sequence.
ACCESSION   DASYNS010000568 DASYNS010000000
VERSION     DASYNS010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3889204"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VGP50_17270"
     CDS             complement(<1..287)
                     /locus_tag="VGP50_17270"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8029188.1"
                     /translation="MFGKTPFIAAAFAALAGTSGVAMAENAPGVTATEIKIGQTMPYS
                     GPASAYAVIGKADAAYFRMINEQGGVNGRKIVLLSEDDGYNPAKAVEATRKL"
     gene            complement(494..3118)
                     /locus_tag="VGP50_17275"
     CDS             complement(494..3118)
                     /locus_tag="VGP50_17275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV8029189.1"
                     /translation="MRDSPRLSPALQAVSVPDSLLRAVLDANLLPMWVEDAATLRILE
                     VNDSALCHYGYGREQFLALTAAALEAPEATSGALAASGVRHHRTADGRVIDMRLETRG
                     TAVDGRPARLVVAIDVTGESQARAENARHRDLLDAAAEWVWEWDAAFRVSHLSPDFAA
                     ATGLLPQSFLGRRQEETAVTAATADQWDGHRATIAAHRRFRDFVFKVAGGAGHAVWLK
                     IGGTPVFDADGAFRGYRGVGSNVTTQVESETALRERERRFRQLFEAASDWYWETDVPG
                     RLTFMSPNFEAMYGVKLVDWLGKRLNDVADARIDPASGQKVLAAIKARQPYSDLVYSL
                     SLPNSRTICVRTSAIPTFDTEGAFSGYLGVSKDITAEVEAERVLRESERQFKQVLEAA
                     ADYYYEQDKQYRFTYLSPGYATRFPIAESLGKRLTEFADLSVEPEMGKLVLLAQKTKQ
                     PYRDFVFSRKTPDGEKHWFKWSGAPLFDRNGQFAGYRGVGAEITQQVEADAAARLAQQ
                     RRLEEAVAYVSHPIVVYDIADRTVAFNQAFTDLHRAPNTNTPVCQGASFREMAEWQLS
                     VRFYGEGPDDAAVDLETLLARHQTEAEHTYHMRDDRWMLVVYRRLPGGGTVGLWTDVT
                     ALKRAEAERRALERQVHHAQRLEALGTLAGGVAHEINNALVPVIALTKLVARKLPDGS
                     RERRSLDVVLTGAERSRDLVKQILAFSRKEEEERRRESVDLGAVLRDVVQLMRATLPA
                     SIRVVDEVAPAPAIIGDPGQLQQVIVNLMTNAAHAIGEAQGKITVRLRPDADGAHLRL
                     SVADTGCGMDEATLARIFEPFFTTKPVGEGTGLGLSMVHGILKDHGGRIEVKSTPGQG
                     TCFDLVLPIPAAQPSKAA"
     gene            complement(3324..>3760)
                     /locus_tag="VGP50_17280"
     CDS             complement(3324..>3760)
                     /locus_tag="VGP50_17280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV8029190.1"
                     /translation="QILADPAQLHQIVINLVVNAAQAIGDKMGKIVFQLAVAPSGALG
                     TAVPATRLSVSDTGCGMDEATRQRIFEPFFTTKAVGEGTGLGLSVVHGIIAGHGGRIT
                     VASTLGEGTRFDILLPAISATEVDEIDDADPGACRHPVRATA"
BASE COUNT          597 a         1252 c         1244 g          667 t
ORIGIN      
        1 agcttccggg tcgcctcgac ggccttggcg ggattgtagc cgtcgtcttc gctcaagagc
       61 acgatcttgc ggccgttgac gccgccctgc tcgttgatca tcctgaaata ggcggcatcg
      121 gccttgccga tgaccgcata agccgaggct ggtccgctat agggcatggt ctgcccgatc
      181 ttgatctccg tggcggtcac gccgggggcg ttctccgcca tggccacgcc gctcgtaccc
      241 gccagcgcag caaatgcggc ggcgatgaag ggtgtcttgc cgaacatgtt cgtctcccta
      301 ttcgagcgat cgagctcttg cgcgatccct ctcgaccaaa ttgagggtga aaactgcatg
      361 atctctcgac gatcgctccg cgctcgagag gcctgcctca agaagcggca atatgggtga
      421 gcttgcttaa gttttagtga aactcgtctc tggctcggcc gagaccccaa aaatgccccg
      481 cgcggaccct ggcctacgcc gccttgctcg gttgcgccgc cgggatcggc aggacgagat
      541 cgaagcaggt gccctggccg ggcgtgcttt tcacctcgat gcggccgcca tggtccttga
      601 ggatgccgtg caccatcgaa agaccgaggc cggtgccctc gccgaccggc ttcgtcgtga
      661 agaagggctc gaagatgcgc gcgagcgtcg cctcgtccat gccgcagccg gtgtcggcga
      721 ccgaaaggcg gagatgcgct ccatccgcgt cgggccggag gcgcacggtg attttccctt
      781 gcgcctcgcc gatcgcatga gccgcgttgg tcatgagatt gacgatgacc tgttgaagct
      841 ggcccgggtc gccgatgatc gccggtgccg gcgcgacctc gtccacgacg cggatgctgg
      901 ccgggagcgt tgcccgcatc agctgcacca catcgcgcag caccgcgccg agatcgacgc
      961 tctcgcggcg ccgctcctcc tcctccttcc gcgagaaggc gaggatctgc ttcaccaggt
     1021 cgcgtgagcg ctcggcgccg gtgaggacga cgtcgaggct gcgccgctcg cggctgccgt
     1081 ccggcaactt gcgcgccacc agcttggtga gtgcgatcac cggaacgagg gcgttgttga
     1141 tctcgtgcgc gacgccgccc gcgagcgtgc ccagcgcctc cagccgctgc gcgtgatgca
     1201 cctgacgctc gagtgcccgt cgctcggcct cggcccgctt cagcgccgtc acatccgtcc
     1261 acagcccgac cgtgcctccg cccggcaggc ggcggtagac gacaagcatc cagcggtcgt
     1321 cgcgcatatg ataggtgtgc tccgcctcgg tctgatggcg ggcgagcagc gtctcgagat
     1381 cgaccgcggc gtcatccggg ccctcgccat agaagcggac gctcaactgc cattcggcca
     1441 tttcgcggaa ggaggcgccc tggcacaccg gcgtgttggt gttcggcgcc ctatgcaggt
     1501 cggtgaaggc ctggttgaag gcgacggtcc gatccgcgat gtcgtagacg acgatgggat
     1561 ggctgacata cgcgaccgcc tcttccaacc gccgctgctg cgccagccgc gccgccgcgt
     1621 cggcttcgac ctgctgcgtg atctccgcac cgacgccgcg atatccggcg aactggccat
     1681 tccggtcgaa gaggggcgcg ccgctccact tgaaccaatg cttctcgcca tccggtgtct
     1741 tgcgcgagaa gacgaagtcg cgataaggct gtttcgtctt ttgcgccagc agcaccagct
     1801 ttcccatctc cggctcgacc gacaggtcgg caaactcggt gagccgcttg ccgagcgatt
     1861 cggcaatggg gaaccgtgtc gcgtaacccg gcgagaggta cgtaaagcgg tattgcttat
     1921 cctgttcgta gtagtagtcg gcggccgcct ccagcacctg cttgaactgc cgctcgctct
     1981 cgcggagtac gcgctcagcc tcgacttcgg cggtgatatc cttggagacg cccagatagc
     2041 cgctgaacgc gccctcagta tcgaaggttg gaatggcact cgttctgacg cagatggtcc
     2101 tgctgttggg caacgagagc gaatagacga ggtcgctgta gggttgccgc gccttgatcg
     2161 cagccagcac tttctgccct gatgcgggat cgatcctcgc gtcggcaaca tcgttgagac
     2221 gcttgccgag ccagtcgacg agctttacgc cgtacatggc ttcgaaattc ggtgacatga
     2281 aggttaggcg ccccggcaca tcggtttccc agtaccaatc ggaggccgct tcgaagagct
     2341 gccggaaacg tcgctcgcgc tcgcgcagcg ccgtctcgga ctcgacctgc gtcgtaacgt
     2401 tgctgccgac cccgcgatag ccgcggaagg cgccgtcggc gtcgaacacc ggcgtcccgc
     2461 cgatcttgag ccagaccgcg tgtcccgccc cgcccgcaac cttgaagacg aaatcgcgga
     2521 agcgccgatg cgccgcgatc gtcgcgcggt gaccgtccca ttggtcggcg gtcgccgcag
     2581 tcaccgccgt ctcctcctgc cgccggccca ggaaggattg cggcaagaga ccggtggccg
     2641 ccgcgaagtc gggcgagagg tggctgacgc ggaacgcggc atcccattcc cacacccact
     2701 ccgctgcggc atcgagcagg tctcggtggc gggcattttc ggcgcgtgcc tggctctctc
     2761 cggtgacgtc gatggcgacg accagccgcg ccgggcggcc gtcgactgcg gtgccgcgcg
     2821 tctcgagccg catgtcgatc acccggccgt cggcggtgcg gtgatgccgg acgccgctcg
     2881 ccgccaaggc gccggacgtc gcttccggcg cctccagcgc ggcggcggtc agggcgagga
     2941 attgctcccg gccgtagccg taatggcaga gcgcgctgtc gttgacctcg aggatgcgca
     3001 gcgtcgccgc gtcctccacc cacatcggca ggaggttcgc gtccagcacg gcgcgtaaga
     3061 ggctatcggg cacgctgacc gcctggagcg cgggcgatag cctggggctg tcccgcattg
     3121 tgggcatccg tgcgtcatcc tctgcgaccg ggctcccata cttccatata gtgattgctc
     3181 aaagcttagc gatcagggag ccggcgactg actcgggtat ggcgttcacc cgtgctggca
     3241 aggcgtcctc gcggaggact cagacgcctt cggcgccggc tcaggatgaa gatgaatcct
     3301 cgacggacaa agaagagacc gcctcaggcg gtcgctcgaa ccggatggcg gcatgcaccc
     3361 ggatcggcgt cgtcgatctc gtcgacctcc gtggcggaga tcgcgggcag aagaatgtcg
     3421 aagcgggtgc cctcgccgag cgtgctcgcc acggtgatgc ggccaccatg cccggcgatg
     3481 atgccatgga cgacggagag gccgaggccg gtgccttcgc ccaccgcctt ggtggtgaag
     3541 aacggctcga agatgcgctg ccgcgtcgct tcgtccatgc cgcagccggt atcgctgact
     3601 gacagacgtg tggccggaac ggccgtgccg agcgcacccg agggcgccac ggcgagctga
     3661 aagacgatct ttcccatctt gtcgccaatg gcctgcgcgg cattgacgac gaggttgatg
     3721 acgatctggt gcagttgcgc ggggtcggcc aggatttgcg
//
LOCUS       DASYNS010000569         6485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2174467, whole genome shotgun sequence.
ACCESSION   DASYNS010000569 DASYNS010000000
VERSION     DASYNS010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174467"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VGP50_17285"
     CDS             <1..814
                     /locus_tag="VGP50_17285"
                     /inference="COORDINATES: protein motif:HMM:NF019829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEV8029191.1"
                     /translation="VQLADLMTLLAAFKLKEGQVRTALSRLVADGWLAAERHGRRSLY
                     RLTPTGRHRFEEATRRIYFGPASEWQGDWHVVVLSKSRPARNEELRKDLGWLGFGTLA
                     AGILLHPAPDRRSLASVIDDLPEAERPLVIAGNAGLPAAPLLARELVARCWDLRALAA
                     AYRHFLAAFTPLRAALEHGLAPAPLPALLARLLLIHDYRRIILRDPMLPPELLPRGWI
                     GGEAYALAGLLYRRLALAAESWIDGHLQGKDGPLPVSDALSQRFPTHYRDRL"
     gene            860..1984
                     /locus_tag="VGP50_17290"
     CDS             860..1984
                     /locus_tag="VGP50_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012554611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8029192.1"
                     /translation="MEPLETNTLPPGIRARLVDGVNGLTMHLLEAGFESAGRPLVLLL
                     HGFPELGYSWRKVMVPLAAAGFHVVAPDQRGYGRTTGWDGAYDGDLASFRLLNLATDA
                     LSLVRALGHRSAAGLVGHDFGAPVAAWCALARPDVFRSLALMSAPFPGPPSLPQGAAA
                     SGDIEEQLARLPRPRKHYHAYYSTRQADGDMQNCPQGVHDFLRAYYHMKSADWRGNQP
                     FPLRSWSAEELAKMPTYYIMDRDSDMPATVAPEMPSRDEITACHWLSESELTVYSSAF
                     RHTGFQGGLQWYRCRTDSRQHSELALFSGRTIDVPSLFIAGKSDWGVYQVPGSLERMR
                     ERACTQMRGCHLIEGAGHWVQQEQPERVSERLIRFLTEAS"
     gene            2136..2834
                     /locus_tag="VGP50_17295"
     CDS             2136..2834
                     /locus_tag="VGP50_17295"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029193.1"
                     /translation="MTLRLRFIATAVASTLSACLTFSAALAATASGKLICFDAETASA
                     PDEDYVASCTEILHRGGGNPRTLARVFQSRAKAYHRMGQDERSVDDLDHAISLDPENS
                     DAWSDRCFGNAVLRRIEPALVDCRKAVAIAPENAEAWSTLGFTRLVQGKDDSALGAYN
                     RALNLAPELPYALFGRGIAKQRQGDQAGGEEDIAAAKALLPDVAAIFMRYGVGPAAEP
                     PPPPGEGAAHITEQ"
     gene            2870..3853
                     /gene="paaA"
                     /locus_tag="VGP50_17300"
     CDS             2870..3853
                     /gene="paaA"
                     /locus_tag="VGP50_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007435392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEV8029194.1"
                     /translation="MYTQGLLQRTEAHPGEDAALVAAFEARVAAEEKIEPNDWMPEAY
                     RRTLVRQISQHAHSEIVGMLPEGNWITRAPSLRRKAGLIAKVQDEGGHGLYLYAAAET
                     LGVSREELVEQLLTGKAKYSSIFNYPTLSWADIGAIGWLVDGAAIMNQIPLCRCSYGP
                     YARAMIRICKEESFHQRQGYEIMLTLCRGTAEQKAMAQDALDRWWWPSLMMFGPSDKE
                     SQHSDASLRWKIKRFSNDELRQKFIDATVPQGLHLGLRFPDPELRFDDASGHWQFGTI
                     DWSEFRAVLAGNGPCNRQRMKQRRDAHEDGVWVREAALAYAEKRRAREGVG"
     gene            4004..4291
                     /gene="paaB"
                     /locus_tag="VGP50_17305"
     CDS             4004..4291
                     /gene="paaB"
                     /locus_tag="VGP50_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEV8029195.1"
                     /translation="MTDKAMQLWEVFIRSRNGLAHKHVGSLHAADAAMALQAARDVYT
                     RRGEGLSIWVVPSTAITASDPADKAMMFEPTASKIYRHPTFYKVPDDVGNI"
     gene            4288..5064
                     /gene="paaC"
                     /locus_tag="VGP50_17310"
     CDS             4288..5064
                     /gene="paaC"
                     /locus_tag="VGP50_17310"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEV8029196.1"
                     /translation="MRAAVATGDSPLFRYALRLADDVLILGHRLSEWIGHAPLLEEEL
                     ALGNIALDLIGEARALYAYAGEVEGAGRDEDALAYRRDVNGFRNLLLVEQPNGDFAAT
                     IVRQFLFSAFVHPFWRALAASHDATLAAIAAKAEKEAAYHLRHAGEWLIRLGDGTTES
                     HRRAQEALDELWTYAGEMFETDGDERALVAQGIAVDRASLRGDWEESVANLLVEATLT
                     RPAAGSWAQSGGRSGRHSEHLGHLLAEMQFLQRAYPRAAW"
     gene            5058..5552
                     /gene="paaD"
                     /locus_tag="VGP50_17315"
     CDS             5058..5552
                     /gene="paaD"
                     /locus_tag="VGP50_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019462221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEV8029197.1"
                     /translation="MVSATDHLRERAWAAAAQVVDPEIPVLSIADLGVLREVRVAGDT
                     VEVVITPTYSGCPAMRVIEFEIAAALGAAGIEKHRITTVLAPAWTTDWLSAEGRRKLA
                     AYGIAPPAGKASRGALFAVDAVACPHCGSAQTERIAAFGSTSCKALYRCRDCAEPFDY
                     FKCH"
     gene            5578..>6485
                     /locus_tag="VGP50_17320"
     CDS             5578..>6485
                     /locus_tag="VGP50_17320"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV8029198.1"
                     /translation="MTPRFHPLTVADLRRETAEAVSIAFAMPEALREAYRFAAGQNLT
                     LKTRLGGEDLRRCYSICSGVDEGELRVAVKRQEGGLFSCFCNDTLRRGDVVEVMTPAG
                     NFTMPLDPAAQRVYLGIAAGSGITPLMSLLKTVLAREPESRFFLLYGNRTTQSIMFRE
                     ALEDLKDRFLGRLSVAHVLSREAQDVPALSGRLDAAKIALFLRTIVPAQSIDHVFLCG
                     PGGLVETAERALRGLGLSPERIHVERFTIDGAPAPKRPQRAESAAAGEIVAEAEAVRD
                     GIRHRFPVGQNQSIIEAAESAGLDLP"
BASE COUNT         1051 a         2178 c         2207 g         1049 t
ORIGIN      
        1 ggtgcagctc gccgatctca tgacgctgct cgcggcgttc aagctcaaag aagggcaagt
       61 gcggacggcg ctgtcgcgcc tcgtcgccga tggctggctc gccgcggagc ggcacgggcg
      121 gcggagcctc tatcggttga cgccgacggg gcgccaccgc ttcgaagagg cgacgcggcg
      181 catctatttc ggaccggcga gcgaatggca gggcgattgg catgtggtgg tcctgtcgaa
      241 gagccggccg gcgcgaaacg aggaattgcg caaggacctc ggatggctcg gcttcggcac
      301 gctggcagcc gggattcttc ttcatcccgc gcccgaccgc cggtcgctcg cctcggtgat
      361 cgacgacctc ccggaagccg agcggccgct ggttattgcc ggcaatgccg ggcttccggc
      421 ggcgccgctt ctcgcgcggg agctggtggc ccgctgctgg gacctccggg cgctggcggc
      481 ggcctatcgc catttcctcg ccgccttcac gccgctccgc gcggcgctcg agcacgggct
      541 cgcgccggcg ccgctgccgg cactgctggc acggctcctg ctcatccacg actaccggcg
      601 cattatcctg cgcgatccga tgctgccgcc cgaattgctg ccgaggggct ggatcggcgg
      661 cgaagcctac gcgctggccg gccttctcta tcgccggctg gcgctcgccg ccgaaagctg
      721 gatcgacggc cacctccagg gaaaagacgg accgcttccc gtcagcgacg ccttgagcca
      781 gcgcttcccg acacattaca gagaccgact gtaaagcggc cgccttgctc cctcgggcgc
      841 gggaagcgag gatggtgcga tggagcctct cgagaccaac acgcttccgc cgggcatccg
      901 cgcgcgcctc gtcgatggcg tgaatggcct cacgatgcat ctgctggagg cgggattcga
      961 gtccgcgggc cgacccttgg tcctcctgct gcacggcttt cccgagctcg gctattcctg
     1021 gcgcaaggtg atggtgccgc tggcggcggc gggcttccat gtcgtggcgc ccgaccagcg
     1081 cggctatggg cggacgaccg gctgggacgg cgcctatgac ggcgacctcg cgtcgtttcg
     1141 cctgctcaac ctcgcgaccg atgcgctgag cctggtcagg gctctcggtc accgctcggc
     1201 cgccggcctg gtgggtcatg atttcggcgc cccggtcgcg gcctggtgcg cgctggcgcg
     1261 gcccgatgtc ttccgcagcc tggcgctgat gagcgcgcct ttcccgggcc cgccaagctt
     1321 gccacaaggg gcggccgcca gcggcgacat cgaggagcag ctggcccgcc tgccgcgccc
     1381 gcgcaagcac taccacgcgt attattcgac gcggcaggcc gatggcgaca tgcagaactg
     1441 cccgcaaggg gtgcatgatt tcctgcgcgc ctactatcac atgaagagcg cggactggcg
     1501 cggcaaccag ccctttcctc tgcggtcctg gagcgccgag gagctggcga agatgccgac
     1561 ctattacatc atggatcgcg acagcgacat gccggcgacc gtcgcgcccg agatgccgtc
     1621 acgcgacgag atcaccgcct gccactggct cagcgagagc gagctcaccg tttacagcag
     1681 cgcgttccgc cacaccggct tccagggcgg cctgcaatgg tatcgctgcc gcaccgacag
     1741 ccggcagcat tcggagctcg cgctcttttc cggccggacg atcgacgtgc cgtcgctctt
     1801 catcgccggc aagagcgact ggggcgttta ccaggtgccg ggcagtctcg agcgcatgcg
     1861 cgagcgcgcc tgcacgcaga tgcgcggctg ccatcttatc gagggcgccg gccactgggt
     1921 gcagcaggaa cagccggagc gcgtcagcga gaggctgatc cggttcctca cggaggcaag
     1981 ctgagcgccc gagccgcctt ccggccggtc cgagaatctt cgtattcaat caataagtta
     2041 cggaaaataa atgagatccg tcatttcctg aggcgacggc tcggcgacgc tccgtttcgc
     2101 cggcgctacg agggacgctt cccgagaaca ccaagatgac tctgcggctc cgcttcatcg
     2161 caactgccgt cgcatcaact ttgtcggcgt gcctcacctt ttcggctgcg ctggcggcaa
     2221 ccgcgagcgg caagctgatt tgcttcgacg cagaaacggc gagtgccccg gacgaagact
     2281 acgtcgcctc ttgcacggag atcctgcacc gcggcggcgg caacccgcga accctcgcac
     2341 gtgtctttca gagccgggcg aaggcctatc accggatggg gcaggacgaa cgatccgtgg
     2401 acgacctgga tcatgcgatc agcctcgatc ccgagaacag cgatgcgtgg agcgacagat
     2461 gcttcggcaa tgccgtgctc cgacgcatcg agccggcgct ggtcgattgc cgcaaggccg
     2521 tggcgatcgc gccggaaaac gccgaggcct ggagcaccct gggcttcacc cgcctggtcc
     2581 agggcaagga cgattcggcg ctcggcgcct ataaccgggc gctcaacctc gcgcccgagc
     2641 tgccctacgc ccttttcggc cgcggcatcg ccaaacagcg ccagggcgat caggccgggg
     2701 gagaggaaga catcgccgcc gccaaagcgc tgctgccgga cgtcgccgcg attttcatgc
     2761 gatatggcgt cggcccggca gccgagcctc cgccgccccc cggcgaaggg gcagcacata
     2821 ttacagaaca gtagtagaaa gctcattttt ctgtgatata ttgtcccgca tgtacacgca
     2881 gggcctgctg cagcggaccg aagcgcatcc cggcgaggat gcggcgcttg tcgccgcttt
     2941 cgaggcgcgc gtcgcggcgg aggagaagat cgagccgaac gactggatgc ccgaagccta
     3001 tcggcgcacg ctcgtccggc agatctcgca gcatgcgcat tccgagatcg tcggcatgct
     3061 gcccgagggc aactggatca cgcgggcgcc gagcttgcgc cgcaaggccg ggctcatcgc
     3121 caaggtccag gacgaaggcg ggcacggact ttatctctat gccgcggccg agacgctggg
     3181 cgtctcacgc gaggagctgg tcgagcaact cctgacgggc aaggcgaagt attcctcgat
     3241 cttcaactac ccgacgctct cctgggcgga tatcggcgcc atcggctggc tggtcgacgg
     3301 cgcggcaatc atgaaccaga tcccgctctg ccgctgctcc tatggcccct atgcgcgggc
     3361 gatgatccgg atctgcaagg aagagagctt ccaccagcgc cagggctacg agatcatgct
     3421 gacgctttgc cgcggcacgg cggagcagaa ggcgatggcg caggatgcgc tcgaccgctg
     3481 gtggtggccg agcctgatga tgttcgggcc ctccgacaag gaatcgcagc attcggacgc
     3541 cagcttgcgc tggaagatca agcgcttctc gaatgacgag ctgcgccaga aattcatcga
     3601 cgccacggtg ccgcaaggtc tccatctggg cttgcgcttt cccgatccgg agctccgctt
     3661 cgacgacgcg agcgggcatt ggcagttcgg caccatcgat tggagcgagt tccgcgccgt
     3721 tctcgccggc aacgggccct gcaaccgcca aaggatgaag cagcggcgcg acgcgcatga
     3781 agacggcgtc tgggtgcgcg aggcggcgct ggcctatgcc gagaagcggc gcgccagaga
     3841 gggtgttggt taggtggaat tgtcacatcc taacctcgtc gtccccgcga aagcggggac
     3901 ccagggcgag cgacgtgccg gcggcccctg gatccccgct ttcgcgggga cgacgaggta
     3961 ggacatggat ttactcaaac ggaaaacgtt ttagagatca gacatgaccg acaaggcgat
     4021 gcagctctgg gaagtgttca tccgctcgcg gaacgggctc gcgcacaagc atgtcgggag
     4081 cctgcacgcg gccgatgcgg cgatggcgct ccaggcggcg cgcgacgtct atacgcggcg
     4141 cggcgaaggg ctctcgatct gggtggtgcc gtcgacggcg atcaccgcgt ccgatcccgc
     4201 cgacaaggcg atgatgttcg agccgacggc gtcgaagatc tatcgccatc cgaccttcta
     4261 caaagtgccc gacgacgtcg ggaacatatg agggcggcgg tggcgacggg cgactctccc
     4321 ctcttccgct atgcgctccg gctcgccgac gacgtgctga tcctgggcca tcgcttgtcg
     4381 gaatggatcg gccatgcgcc gctgctcgag gaagagctgg cgctcggcaa catcgcgctc
     4441 gatctcatcg gcgaagcgcg ggcgctctat gcctatgccg gcgaggtcga aggcgcgggg
     4501 cgcgacgagg acgcgctcgc ctaccggcgc gacgtgaacg gctttcgcaa tctgctgctg
     4561 gtcgagcagc ccaatggcga cttcgccgcg accatcgtgc ggcaattcct cttttccgcc
     4621 ttcgtccatc ccttctggcg cgcgctcgcg gcgtcccacg acgcgacgct tgccgcgatc
     4681 gcggcgaagg ccgagaaaga agccgcctat catctgcgtc atgccggcga atggctgatc
     4741 cggctcggcg acggcacgac ggaaagccat cgccgcgcgc aagaggcgct cgacgagctc
     4801 tggacctacg cgggcgagat gttcgagacg gatggcgacg agcgcgcact ggtcgcgcaa
     4861 ggcatcgccg tcgaccgcgc gtcgctccgc ggcgattggg aggagagcgt cgccaacctg
     4921 cttgtggaag cgacgctcac gcggcccgcc gcggggagct gggcgcagag cggcggacgc
     4981 agcgggcgcc attcggagca tctgggccat ctgctcgcgg agatgcagtt cctgcagcgg
     5041 gcatatccga gagcggcgtg gtgagcgcga ccgatcacct gcgcgagcgc gcctgggcag
     5101 cggcggcgca agtcgtcgat cccgagattc ccgtgctcag cattgccgat ctgggcgtgt
     5161 tgcgcgaggt ccgcgtcgcc ggcgacaccg tcgaggtggt gatcacgccg acctattccg
     5221 gatgcccggc gatgcgcgtg atcgaattcg agattgcggc ggcgctgggc gcggccggca
     5281 tcgagaagca ccggatcacg acggtgctgg cgccggcctg gacgacagat tggctgagcg
     5341 cggagggtcg gcgcaagctc gccgcctatg gcatcgcgcc gcccgccggc aaggcgtcgc
     5401 gcggtgcgct tttcgccgtc gacgcggtcg cgtgcccaca ctgcggctcg gcacagaccg
     5461 agcggatcgc ggccttcggc tccacgtcgt gcaaggcgct ctatcgctgc cgcgactgcg
     5521 ccgagccttt cgactatttc aaatgccatt gaatcatcga ccggtcgcgg gcacgcgatg
     5581 accccgcgtt ttcatccgct caccgtcgcc gatctgcggc gcgagacagc ggaggccgtc
     5641 tcgatcgcgt ttgccatgcc ggaagcactg cgcgaagcgt atcgcttcgc ggctgggcag
     5701 aacctcactt tgaagacgag gctcggcggc gaggatttgc gtcgctgcta ctcgatctgc
     5761 tcgggcgtcg atgagggcga gctgcgcgtc gccgtcaagc ggcaggaggg cgggctcttc
     5821 tcctgcttct gcaacgatac gctgcggcgc ggcgatgtcg tcgaggtcat gacgccggcg
     5881 gggaacttca ccatgccgct cgatcccgcg gcacagcgcg tctatctcgg catcgccgcg
     5941 ggctccggca tcacgccgct gatgtcgctc ctcaagacgg tgctggcgcg cgagcccgag
     6001 agccgcttct tcctgctcta tggcaaccgc acgacgcaga gcatcatgtt ccgcgaagcg
     6061 ctcgaggatc tgaaggaccg cttcctcggc cgcttgtcgg tagcgcatgt gctgagccgc
     6121 gaggcgcagg acgtgccggc gctgagcggg cgcctcgacg cggcgaagat cgcgctcttc
     6181 ctgcgcacga tcgtgccggc gcagagcatc gaccacgtgt tcctgtgcgg ccctggcggc
     6241 ctcgtcgaga cggcggagcg ggcgcttcgc gggctcggcc tgtcgcccga gcgcatccat
     6301 gtcgagcgct tcaccatcga cggcgcgcca gcgccgaagc gtccgcagcg cgcagagagc
     6361 gccgcggccg gggagatcgt cgccgaagcg gaggccgtcc gcgacggcat ccgccaccgc
     6421 ttccccgtgg gtcaaaacca atcgatcatc gaggcggccg agagcgccgg gctcgacctc
     6481 cccta
//
LOCUS       DASYNS010000570         5529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_146946, whole genome shotgun sequence.
ACCESSION   DASYNS010000570 DASYNS010000000
VERSION     DASYNS010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146946"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VGP50_17325"
     CDS             <1..212
                     /locus_tag="VGP50_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV8029199.1"
                     /translation="GFRALPLMEDVDLVRRLGRERLVQLPTDAITSAARYRREGYVSR
                     PLRNLGCLTLYFLGLPPRFILRLYG"
     gene            222..914
                     /locus_tag="VGP50_17330"
     CDS             222..914
                     /locus_tag="VGP50_17330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEV8029200.1"
                     /translation="MRRAQHLVIFLRAPRLGRVKSRLARGIGALAALRFYRNTVDALL
                     RRVTGNRRWRLELAVTPDIDARRRPWRVAAGYRAQRRGDLGARMARVFRDLPPGPAVI
                     IGSDIPALGPAHIAAAFNALGRHDAVFGPASDGGYWLVGLRRRPRLPPRLFKSVRWSS
                     EHALGDTLAGLPRTFSVAFLETLEDVDDVESYRRWRRSAALSRQRASDARSIAASARR
                     GCRDGDGCRADA"
     gene            complement(823..1371)
                     /gene="moaB"
                     /locus_tag="VGP50_17335"
     CDS             complement(823..1371)
                     /gene="moaB"
                     /locus_tag="VGP50_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEV8029201.1"
                     /translation="MPRIDEARPFLAVNIAILTVSDTRSESDDKSGQTLAELIAAAGH
                     RVAQRRIVRDEMAAIIAQLKAWIVDPTIDVVISTGGTGVTGRDVTPEAFHAVYEKEIA
                     GFGELFRWLSYQKVGTSTIQSRATAGVAGGTYLFALPGSPGACRDGWEDIIKHQLDNR
                     HRPCNLVELMPRLNEHLKPAVG"
     gene            complement(1355..2869)
                     /locus_tag="VGP50_17340"
     CDS             complement(1355..2869)
                     /locus_tag="VGP50_17340"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEV8029202.1"
                     /translation="MSTGAAAQSIRTSALTPGTEPPPEADLPSALGDSDLRHYQRIFR
                     LQAAAEWAAADREIAALDDRLLIGTVEAQRYLHPAYRPNYTELSHWLARHAEEPDAKA
                     IYALALKHRPANAVAMPMRPAVAFLVPRGLGEEQFETAAFADWEAGLAAWRRGRVGDA
                     RRRFETLAARHDLSSAMTAAAAFWAARTELRGNRPSSFTDWLGVAAEHPRTFYGLLAR
                     RILGTDGDLGLESDPFTDIDAQLLLDTPAGRRALALLQISDTFRAEAELRSLAPRASP
                     LLIEALVAVADRANMPALSLQLAGLLNDGAGRHHALYPLPRWTPVGGFTVDRALLFAV
                     MRQESQFLPHVESPAGAVGVMQLMPATAHAMARRAGVLLDASNRRQLREALADPEINM
                     ALAQEYIGELRESAGIGDNLLLLAAAYNCGPSGPQRWQADPQLRDDPLLFLESIPRRE
                     TRIFTERVLANYWIYRQRLGQPTPDLDALAAGEWPTYTALDLGPEPERRHAQNR"
     gene            complement(3073..3852)
                     /locus_tag="VGP50_17345"
     CDS             complement(3073..3852)
                     /locus_tag="VGP50_17345"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV8029203.1"
                     /translation="MSETGSAAELLRWYVAMGADEAIAEAPIDRLAAAASVVPPSPPP
                     RAAPPVIVTEAPAGTLSARQLAAAAKDLGELAAAVHAFDGCALKRTATNTVVADGRPG
                     ARLMVIGEAPGAEEDRLGRPFVGRSGQLLDRMLAAIGFDRRENAYITNVLFWRPPGNR
                     KPNADEIAACLPFVWRQIALGAPEAVVMCGGTATSALLGRAEGIMRLRGRWFELTGPG
                     LDRPVPALATYHPSFLLRAPARKGEAWRDLLALQSKLGSAQ"
     gene            3948..>5529
                     /locus_tag="VGP50_17350"
     CDS             3948..>5529
                     /locus_tag="VGP50_17350"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEV8029204.1"
                     /translation="MPRERMEYDVVIVGAGPSGLAAAIRLRQLSQQHGREISVCVIEK
                     GSEVGAHILSGVLIDPIAINELLPDWKAQGAPLNTPVSEDRVLLLGERGHIRFPSFLM
                     PPLMHNHGNYVASLANVCRWLAKQAEALGVEIYSGFAGAEILYDDDGSVRGVATGDMG
                     VARDGHHKNNYTPGVELVAKYTLFAEGARGSLTKMLMERFKLCEGVDPQKFGIGIKEI
                     WQIDPAKHKPGLVMHTLGWPLGHRTGGGSFLYHFDDHQVSIGLVVHLNYENPHLSPYD
                     EFQRLKLHPAIRPYLDGGKRLAYGARALTEGGVQSVPKLVFPGGALIGCTAGFMNVAR
                     LKGSHNAMKSGMLAAEAAFAALADGGRRRELTDYPEALRRSWIYKDLWKVRNFKPGLS
                     NWGTWLGMVHGGLHMWLGELGLGFLVPYTLHHRKADHETLKPADQCPRIDYPRPDGQI
                     TFDKLSSVFLSNTNHEEDQPIHLVLRDQSIPVSVNLPRYDAPEQRYCPAGVYEIVREN
                     GAKPRLQINAPNCVHCKTC"
BASE COUNT          893 a         1845 c         1868 g          923 t
ORIGIN      
        1 gcggctttcg cgctttgccg ctcatggagg acgtcgatct cgtgcggcgc ctcggccgcg
       61 agcggctggt tcaactgccg accgatgcga tcacttcggc ggcgcgctat cgccgggagg
      121 gctatgtctc gcgtccgctg cgcaacctcg gttgcctcac gctgtatttc ctcggactgc
      181 cgccgcgttt cattctgcgg ctctatggct gaataccctc gatgcgccgc gcccagcatc
      241 tcgtgatctt cctgcgcgcg ccgcgattgg gccgcgtcaa gtcgcgtctc gcccgcggca
      301 tcggcgctct cgccgcgctg cgcttctatc gcaataccgt cgacgctttg ctgcgacggg
      361 tcaccggcaa tcggcgctgg cgtctggaac tcgccgtcac gcccgacatc gacgcgcgtc
      421 gccggccgtg gcgcgtcgcc gcaggctatc gcgcccagag gcgcggtgat ctcggcgcgc
      481 gcatggcgcg cgtgtttcgc gatttgccgc cggggccggc cgtcatcatc ggctccgaca
      541 ttcccgcgct cgggccggcg catatcgccg ccgcgttcaa cgcgctgggc cggcatgacg
      601 ccgtcttcgg tccggcgagc gacggcggct attggctggt gggcttgcgg cgccggccgc
      661 gcctgccgcc gcgattgttc aagagcgtgc gctggtcgag cgagcatgcg ctcggcgata
      721 cgctcgccgg cttgccgcgc accttctctg tggcgtttct cgaaacgctc gaagacgtcg
      781 acgatgtcga atcctacagg cgctggcggc gcagcgccgc gctcagccga cagcgggctt
      841 cagatgctcg ttcaatcgcg gcatcagctc gacgaggttg cagggacggt gacggttgtc
      901 gagctgatgc ttgatgatgt cctcccagcc gtcgcggcag gcgccgggag aaccgggaag
      961 cgcgaagaga taagtgccgc cggcgacgcc ggcggtggcg cggctctgga tcgtcgacgt
     1021 gccgactttc tgatagctga gccagcggaa gagttcgccg aagccggcga tctccttctc
     1081 gtagacggcg tgaaacgctt cgggcgtcac gtcgcggccg gtgacgccgg taccacccgt
     1141 cgaaatcacg acatcgatcg tcggatcgac gatccacgcc ttgagctgcg cgatgatcgc
     1201 ggccatctcg tcgcgcacga tgcgacgctg cgcgacgcgg tggccggcgg cggcgatcaa
     1261 ctcggcaagc gtctggccgg atttgtcgtc gctctcgctg cgcgtgtcgg agacggtgag
     1321 gatcgcgata ttgacggcga ggaacggccg cgcttcatcg attctgggca tggcgacgct
     1381 ccggctcggg gccgagatcg agcgcggtat aggtcggcca ttcaccggcg gcaagggcgt
     1441 cgaggtcggg tgtcggctgc ccgaggcgct ggcggtagat ccaataattc gcgagaacgc
     1501 gctcggtgaa aatgcgcgtc tcgcgacgag ggatgctctc gaggaagagc agcggatcat
     1561 cgcgcagctg cggatcggcc tgccagcgct gcgggccgct cggcccgcaa ttgtaggccg
     1621 cggccagcag cagcagattg tcgccgatgc cggcgctctc tcggagctcg ccgatatatt
     1681 cctgcgccag cgccatgttg atctcgggat cggcgagcgc ctcgcgcaat tgccgtcggt
     1741 tgctcgcatc gagcagcacg ccggcgcgcc gcgccatggc gtgagccgtt gcgggcatca
     1801 actgcatgac gccgaccgcg cccgccgggc tttcgacatg cggcaggaat tgcgactctt
     1861 gccgcatcac ggcgaagagc agcgcgcgat cgacggtgaa gccgccgacc ggcgtccagc
     1921 gcggcagcgg atagagcgcg tgatggcgtc cggcgccgtc gttcaaaagg ccggcgagct
     1981 gcaggctcaa ggcaggcatg ttggcgcggt cggcgaccgc gaccaacgct tcgatgagga
     2041 gcggcgaggc gcgcggcgcg agcgagcgca actccgcctc ggcgcggaaa gtgtcgctga
     2101 tctgcagcag ggcgagcgcg cggcggccgg ccggcgtgtc gagcaagagc tgtgcgtcga
     2161 tgtcggtgaa cggatcgctc tcgaggccga ggtcgccgtc ggtgccgaga atgcgccggg
     2221 cgaggaggcc gtagaaggtg cggggatgct cggcggcgac gccgagccaa tcggtgaagg
     2281 agctcggtcg gttgccgcga agctcggtgc gcgccgccca gaaggccgcc gccgccgtca
     2341 tggcgctcga gagatcgtgc cgggcggcga gggtctcgaa gcggcgccgc gcatcgccga
     2401 cgcgaccccg gcgccaagcc gcgagcccgg cctcccagtc ggcaaaagcc gccgtttcga
     2461 attgctcctc gcccagaccg cgcgggacga gaaacgcgac cgccggccgc atcggcattg
     2521 ccacggcgtt cgccgggcga tgcttcagcg ccagggcata gatcgccttg gcatcgggct
     2581 cttcggcgtg gcgggcgagc cagtgggaga gctcggtata gttggggcga taagccgggt
     2641 gcagataacg ctgcgcctcg accgtgccga tcagcagccg gtcgtcaagc gccgcgatct
     2701 cgcggtcggc cgccgcccac tccgccgccg cctgcaatcg aaagatgcgc tgatagtgcc
     2761 ggagatcgct gtcgccgagc gccgatggca aatccgcctc cggcggcggc tcggtgccgg
     2821 gggtgagcgc cgaggtgcgg atcgattgcg ccgcagcacc cgtggacacg gttgtcgcaa
     2881 ggcctggcaa taccgtggca agggcaacaa aagcaccgac cctcacccaa gtcctgccgg
     2941 gccggaactc gtatctcctg ccctccatga ctaaagccaa tagattattg ggctcgacct
     3001 ggcaagctaa aatgcgcgtc aaatttgagc caattgttaa tatccccagg aatgtttgta
     3061 gattccccga ggttactgtg ccgagcccag tttcgattgg agggccagca aatcccgcca
     3121 ggcttcgcct tttcttgccg gcgcgcgcag caagaacgac gggtgatagg tcgcgagcgc
     3181 tggcaccgga cgatcgaggc cgggacccgt cagctcgaac cagcggccgc gcagccgcat
     3241 gatcccttcg gctcgcccga gaagagcgct ggtcgccgtg ccgccgcaca tgacaacggc
     3301 ttccggcgcg ccaagggcga tctgccgcca gacgaaaggc aggcaggcgg cgatctcgtc
     3361 ggcgttcggc ttgcggttgc cgggcggccg ccagaacagc acgttggtga tgtaggcgtt
     3421 ctcccggcga tcgaagccga tagcggccag catgcgatcg agcagctgac cgctgcggcc
     3481 gacgaaaggc cggccgagcc gatcctcttc ggcacccggc gcctcgccga tcaccatcaa
     3541 ccttgctccg ggccgtccgt cggcgacgac ggtgttggtc gcggtgcgct tcaaggcaca
     3601 gccgtcgaag gcgtggaccg ctgcggcgag ctcgccgaga tccttggcgg cagcggcgag
     3661 ctggcgtgcg ctcagtgtac cagccggcgc ctcggtgacg atgaccggcg gcgcggcgcg
     3721 cggcggcggt gacggcggca cgacgctcgc cgctgccgcc aagcggtcga tcggcgcttc
     3781 ggcgatcgcc tcgtcggcgc ccatcgcgac gtaccagcgc aagagttctg cggcagatcc
     3841 cgtctcgctc atgccgtcaa ccctagcgcg acgccggcag agacgcgagc gcctctggct
     3901 ctttgggcat catcgactat agtggcccaa atttgcgggg aaggctgatg ccgcgcgagc
     3961 ggatggaata tgacgtggtc atcgtcggcg ccggcccttc cgggctcgcc gcggcgatac
     4021 gcttgcggca actgagccag cagcacggcc gcgagatctc ggtctgcgtc attgagaagg
     4081 gctcggaggt cggtgcgcat attctctcag gcgtcctcat cgatccgatt gcgatcaacg
     4141 agctgctccc cgactggaag gcgcagggcg caccgctcaa cacgccggtg agcgaggatc
     4201 gcgtcctctt gctcggcgag cgcggccata tccgctttcc cagtttcctc atgccgccct
     4261 tgatgcacaa tcacggcaac tacgtcgcga gtcttgccaa tgtctgtcgc tggctcgcga
     4321 agcaggcgga ggcgctcggc gtcgagatct attcgggctt cgccggcgcc gagattctct
     4381 acgacgacga cggcagcgtc agaggcgtcg ccacgggcga catgggcgtg gcgcgcgacg
     4441 gccatcacaa gaacaactac acgccgggcg tcgaactcgt cgccaaatac accctcttcg
     4501 ccgaaggagc gcgaggctcg ctcaccaaga tgctgatgga gcgcttcaag ctgtgcgagg
     4561 gcgtcgatcc gcagaagttc ggcatcggca tcaaggagat ctggcaaatc gacccggcca
     4621 agcacaagcc cggcctggtg atgcacacgc tcggctggcc gctcggtcac cggacaggcg
     4681 gcggctcctt cctatatcac ttcgacgacc atcaggtatc gatcggcctc gtcgtgcatc
     4741 tcaactacga gaacccgcat ctttcgccct acgacgagtt ccagcgcctg aagcttcatc
     4801 cggccatccg cccctatctc gacggtggta agcgccttgc ctatggcgcg cgtgcgctga
     4861 ccgagggcgg agtgcaatcg gtgccgaagc tcgtctttcc cggcggcgcg ctcatcggct
     4921 gcaccgccgg cttcatgaac gtcgcgcgcc tcaagggttc gcacaacgcg atgaagagcg
     4981 gcatgctcgc cgccgaggcc gccttcgccg ccctcgcgga cggtggtagg cgccgcgagc
     5041 tcaccgacta tcccgaggcc ttgcgccggt cttggatcta caaggacctg tggaaggtgc
     5101 gcaacttcaa gccgggcctg tcgaactggg gcacgtggct cggcatggtt catggcggcc
     5161 tccatatgtg gctcggcgaa ctcgggcttg gatttctcgt cccttacacg ctccatcatc
     5221 gcaaggccga tcacgagacg ctgaaaccgg cagaccaatg tccgcggatc gactatccca
     5281 ggcccgacgg ccagatcacc ttcgacaagc tgtcctctgt cttcctctcc aacaccaatc
     5341 acgaagagga tcagccgatt catctcgtcc tcagggatca atcgattccg gtttccgtta
     5401 accttccgcg ctatgatgcg ccggagcagc ggtactgccc ggccggcgtc tacgagatcg
     5461 tccgcgagaa cggcgccaag ccgcgcctgc agatcaacgc gccgaactgc gtccactgca
     5521 agacctgcg
//
LOCUS       DASYNS010000571         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4670107, whole genome shotgun sequence.
ACCESSION   DASYNS010000571 DASYNS010000000
VERSION     DASYNS010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4670107"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="VGP50_17355"
     CDS             <1..1666
                     /locus_tag="VGP50_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEV8029205.1"
                     /translation="ITKGSGRIAEVAARFSLDSMPGKQMAIDADLSAGLINEDTARKR
                     RTDLEDESNFYGAMDGASKFVRGDAVAGLLITVINIVAGIVIGVIQKGVSFGDATQTY
                     TLLTVGDGLVTQIPALIVSTAAGMLVSKAGVAGSTDKALFGQLSAYPAALGVVSFLML
                     ALAMVPGIPLLPFLALSGLTGGAAWRLTEKKERAKAQAAAQQTVAAAPGKEEPIQTAL
                     QIDQLRLELGYGLLGLINSNQGARLTDQVRALRRQLAGEMGFVMPSVRIQDNLQLPTN
                     VYVLRVKEIEAGRGDLRPNMLLVMDPRGGAIAVAGEPTVEPTFGLPAQWIPEASREEA
                     TFRGYTVVEPQTVITTHLTEVIKDNMAELLSYAETQKLLDELDKSHQKLISDMVPSQI
                     SVGGIQRVLQNLLAERVSIRDLPTILEGIAEACGSSRNISVVTEHVRARLARQISETN
                     TSEAGFIPLIGLSPVWEQSFAEALTGQGDERHLSMPPSRLQEFIGAVRQTFERHAMLG
                     ETPVLMTSAAIRPYVRSIIERFRPITVVMSQNEVHPKARIKTVGTI"
     gene            1674..2633
                     /locus_tag="VGP50_17360"
     CDS             1674..2633
                     /locus_tag="VGP50_17360"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEV8029206.1"
                     /translation="MRLSTFTAPTMTEAMSLVRARLGEDAIIVSTEEDEDGNARVTAA
                     VERPERPLPAAGPDVVDVLNEALSSHGLSPVLIEKILCTALTFHTDDPLLALAGALTA
                     LLRFTPVESAPRRPLLLIGPPGAGKTLALAKLAARAVLAGTTPRLVTADTVRAGAAEQ
                     LEAFARVLKLAVHRATTAARLGPLVAASKTSDLLLIDSAGINPYNAADRRELAAIIAA
                     SGAEPVLVLPAGGDAVDTLETARIFRDLGAERVIVTRLDMTQRLGSVMGAAEALRLAF
                     SEASATADVANGLTPCNAVVLARLLLPHPAETLRRANTRKASP"
     gene            2630..>3184
                     /locus_tag="VGP50_17365"
     CDS             2630..>3184
                     /locus_tag="VGP50_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008614754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8029207.1"
                     /translation="MKPTSVVAAFPQPGAAPHRRNIIAVASGKGGVGKTWFSITLAHA
                     LAKAGRKTLLFDGDLGLANVDVQLGFTPERDLGSVIAGDATLAQVASAYEGGFDIVAG
                     RSGSGSLATLPPSRLLALRAELQLFAQNYDWVVLDLGAGIERTVRLLTGQSRACIVLA
                     TDEPTAITDAYAYIKVTALERLAEG"
BASE COUNT          522 a         1162 c         1016 g          484 t
ORIGIN      
        1 catcaccaag ggctcggggc gcatcgccga agtcgcggcg cgcttcagct tggacagcat
       61 gccgggcaag cagatggcga tcgacgccga cctttccgcc ggcctcatca acgaggacac
      121 ggcgcggaag cggcgcaccg acctcgagga cgagagcaat ttctatggcg ccatggacgg
      181 cgcctcgaaa ttcgtccgcg gcgatgccgt ggccggcctc ctcatcaccg tcatcaacat
      241 cgtcgccggc atcgtcatcg gcgtcatcca gaaaggcgtg tccttcggcg acgcgactca
      301 gacctatacg ctgctcaccg tcggcgacgg cctggtgacg cagattccgg cgctcatcgt
      361 ctcgaccgcg gccggcatgc tcgtctccaa ggccggcgtc gcaggctcga ccgacaaagc
      421 gctcttcggt cagctctcgg cctatccggc agcactcggc gtcgtctcct tcctgatgct
      481 ggcgctggcc atggtcccgg gcattcccct cctccccttc ctcgccctct cgggcctgac
      541 cggcggcgcc gcctggcgcc tcaccgagaa gaaagagcgg gcgaaggcgc aggccgcggc
      601 acagcaaacc gtcgccgcgg cgcccggcaa ggaagagccg atccagacgg cgctgcagat
      661 cgaccagctg cgcctcgagc tcggctatgg cctcctcgga ctcatcaaca gcaatcaagg
      721 cgcgcgcctc accgaccagg tgcgggcgct gcgccgccag cttgcaggcg agatgggctt
      781 cgtcatgccg tcggtgcgca tccaggacaa tctccagctt ccgaccaacg tctatgtcct
      841 tcgcgtcaag gaaatcgaag cggggcgagg cgatcttcgc cccaacatgc tgctggtgat
      901 ggacccgcgc ggcggcgcca ttgccgtggc cggcgagccg acggtcgagc cgaccttcgg
      961 actgccggcg caatggatcc ccgaggcgag ccgcgaggag gcgacgttcc gcggctacac
     1021 cgtggtcgag ccccagacgg tgatcaccac gcatctgacc gaggtgatca aggacaacat
     1081 ggccgagctc ctatcctatg ccgagacgca gaagctcctc gatgagctcg acaagagcca
     1141 tcagaagctc atctccgaca tggtgccgag ccagatctcc gtcggcggca tccagcgcgt
     1201 gctgcagaac ctcctcgccg agcgggtatc gatccgcgat ctgccgacca ttctcgaagg
     1261 catcgccgag gcctgcggct ccagccgcaa catcagcgtc gtcaccgagc atgtgcgggc
     1321 gcggctcgca cggcagatca gcgaaaccaa caccagcgag gccggcttca tcccgctcat
     1381 cggactatcg cccgtctggg aacagtcctt tgccgaggcg ctgaccggcc agggcgacga
     1441 gcgccatctc agcatgccgc cgagccggct ccaggagttc atcggcgccg tgcggcagac
     1501 cttcgagcgc cacgcgatgc tcggcgagac cccggtgctg atgacgagcg cggcgatccg
     1561 gccctatgta cgctcgatca tcgaacgatt ccgtccgatc accgtggtga tgtcgcagaa
     1621 cgaagtgcat ccgaaggcgc gcatcaagac cgtcggcacg atctgagtaa cgcatgcgtc
     1681 tctccacctt caccgcgcca acgatgaccg aggccatgag cctggtgcgc gcgcgcctcg
     1741 gcgaggacgc gatcatcgtc tccaccgagg aggacgaaga cggcaacgcc cgtgtcaccg
     1801 cggcagtcga gcggccggag cggccactcc ccgccgctgg cccggatgtc gtggacgtcc
     1861 tgaacgaggc gctgagcagc catggcctca gcccggttct catcgagaag attctctgca
     1921 cggcgttgac gttccatacc gacgacccgc tgctggcgct ggcgggggcg ttgacggcgc
     1981 tgctgcgatt cacgcccgtc gagtccgcgc cgcgccgccc gctgctgctg atcgggccgc
     2041 ccggcgccgg caagactttg gccctcgcca agcttgcggc tcgggccgtg ctggccggta
     2101 cgacgccgcg cctcgttacc gcggacaccg tgcgcgccgg cgctgccgag cagctcgagg
     2161 ccttcgctcg cgtgctgaag ctggcggtgc atcgcgccac caccgccgcg cgcctcggcc
     2221 cgctggtcgc ggccagcaag accagcgacc tcctgctcat cgattccgcc ggcatcaatc
     2281 cctacaatgc cgccgatcgg cgtgagctcg ccgcaatcat cgccgccagc ggcgccgagc
     2341 cggtgctcgt gctgccagcc ggcggcgatg ccgtcgacac gctcgagacg gcgcgcatct
     2401 tccgcgacct tggtgccgag cgcgtcatcg tcacccgcct cgacatgacg caacgcctgg
     2461 gcagcgtcat gggtgcggcc gaggcgttgc gtctcgcgtt cagcgaggcg agcgccaccg
     2521 ccgacgtcgc gaacggacta actccctgca acgccgtggt gctcgcgcgc ctgctcctgc
     2581 cgcacccggc agagacgctg cgccgggcca ataccaggaa agcatcgcca tgaagccaac
     2641 atccgtggtg gcggcgtttc cgcagcccgg cgccgcgccg catcgccgca acatcatcgc
     2701 cgtcgcttcg ggcaaaggcg gcgtcggcaa gacctggttc tcgatcaccc tcgcgcatgc
     2761 cctggccaag gcaggacgca agacgctgct cttcgacggc gatctcggcc tcgccaatgt
     2821 cgacgtgcag ctcggcttca cgcccgagcg cgatctcggc agcgtcatcg ccggcgacgc
     2881 gacgctggca caggtggcca gcgcttatga gggtggattc gacatcgttg cggggcgctc
     2941 cggctccggc agcctcgcga cgcttccgcc gagccggctc ctggcgctgc gggcggagct
     3001 gcagctcttc gctcagaact acgactgggt cgtgctcgat ctcggcgccg gcatcgagcg
     3061 cacggtgcgg ctgctcaccg gacaatcacg cgcctgcatc gtcctcgcca ccgacgagcc
     3121 cacggccatc accgacgcct acgcctatat caaggtcacg gcgttggagc ggctggccga
     3181 gggc
//
LOCUS       DASYNS010000572         4709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_304350, whole genome shotgun sequence.
ACCESSION   DASYNS010000572 DASYNS010000000
VERSION     DASYNS010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304350"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VGP50_17370"
     CDS             <1..522
                     /locus_tag="VGP50_17370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8029208.1"
                     /translation="METTFALWANQVFGWGSWEVGLNFFFVGVVLVVVQGGLIGWLSR
                     RLGEARLLTAGALSIMLGLAGLPLVHAIPLLLVVNLLLAAGMGLLNPSLNSLISRQAR
                     IDEQGGILGVSQSASSLARAAAPPLAGLLFDTSGRNTPYIVGAALMLLVLILALRLPR
                     VASDATMSEVLPS"
     gene            519..2357
                     /locus_tag="VGP50_17375"
     CDS             519..2357
                     /locus_tag="VGP50_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEV8029209.1"
                     /translation="MKGVAAPVGNKPSEVGLRQELKAVRGLFPYLWPREWDLRARVLL
                     SLAFLVAAKLVSVYIPLLYKGAVDALSPGRSTAIVVPVALIVFYGLGRVGAQLFAEVR
                     DAVFAKVGQRAVRRVALATFRHMHALSLRFHLERQTGGLSRAIERGIRGTEFLLSYLL
                     FNVVPTLFEILLVCGILWSFYDASFALVTLAVIVAYIVFTFTVTDWRVKFRREMNERD
                     SEANTKAIDSLLNFETVKYFANEEHEAQRFDRALRAYERAAVKSQTTLSALNIGQGVI
                     IAAGLVVVMLLAGRGVARGSMTVGDFVLVNAYLVQLYAPLNVLGMVYRNIKQSLTDLE
                     SMFRLLGVRAEIEDRPGAPPLRIGRGEIVFDHVSFRYDQRRPILHDVSFRVPPGGTLA
                     IVGPSGAGKSTIARLLFRFYDVDSGAIRIDGQDLRAVTQESLRQAIGVVPQDTVLFND
                     TIRYNIAYGRTGASDEEIVAAARHARIADFVASLPDGYRSMVGERGLKLSGGEKQRVA
                     IARVILKEPSILVFDEATSALDTKTEQEIQASLDEVRSQRTTLVIAHRLSTVVNADEI
                     LVLDEGRIVERGNHALLLAQGGVYAAMWARQQELDEREAALAAVAE"
     gene            2363..3403
                     /locus_tag="VGP50_17380"
     CDS             2363..3403
                     /locus_tag="VGP50_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV8029210.1"
                     /translation="MAAQAPSGDKQTINLALQGGGTHGAFTWGVLDRFLEDERLAIEG
                     ISGTSAGAVNAAVLAQGHAKGGSAGARAELERFWRRMSELGWISPVHRGVLERLQGTW
                     NLDGSPSSILVDQMTRLVSPYQFNPLNLSPLRDVLSSTLDWTTLTAPDSIKLFITATN
                     VETGKPRVFARQDLTLEALLASTALPQLFQAVVIDGTPYWDGGYMGNPSIWPLIYFCQ
                     SHDVVIVQVNPLTRKGVPRSAAEITNRLNEITFNTPLMGEMRAISFVQRLLAGDSANP
                     EVSRLKNMLIHMVGDEDAMEQLGVASKLNTDLDFLLYLRDLGRRTAERWLDENFAALN
                     QRSSFDVRKVFL"
     gene            3457..4461
                     /gene="meaB"
                     /locus_tag="VGP50_17385"
     CDS             3457..4461
                     /gene="meaB"
                     /locus_tag="VGP50_17385"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEV8029211.1"
                     /translation="MARPAAAPKELATGLRGGDRRALSRAITLVESSRDDHRAEAEEL
                     LAELLPASGGALRLGISGAPGVGKSTFIEAFGLAAIGAGHRVAILAVDPSSKRGGGSI
                     LGDKTRMPELARHGDAYIRPSPAGKTLGGVARRTRDAITLAEAAGFDVVIVETVGVGQ
                     SETAVADMVDLFLLLLAPGGGDDLQGIKKGIVELADLLVVNKADGDQKPAALRAVGDY
                     RHALRMLRPAAADEAMPLVLAVSALTGDGIPQIWERVEHQAATLLASGERERRRARQA
                     RAALWSEIGEGLVDLFKADQHVAAKLGDIEARVAEGTLTPSAAARAALAAFRGRARAT
                     "
     gene            4548..>4709
                     /gene="rpmB"
                     /locus_tag="VGP50_17390"
     CDS             4548..>4709
                     /gene="rpmB"
                     /locus_tag="VGP50_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV8029212.1"
                     /translation="MARRCIITGKGVQVGNNVSHANNKTKRRFLPNLQVTSLLSDALG
                     APIRLRLSTH"
BASE COUNT          712 a         1624 c         1539 g          834 t
ORIGIN      
        1 atggagacca ccttcgcgct ctgggccaac caggtcttcg gctggggctc ctgggaagtg
       61 ggcctcaact ttttcttcgt cggcgtcgtc ttggtggtgg tgcagggcgg gctgatcggt
      121 tggctgtcgc gccgcctcgg cgaggcgcgg ctgctcacgg cgggtgcgct gtcgatcatg
      181 ctggggctcg ccgggctgcc gctcgtgcac gcgattccgt tgctgctggt cgtcaacctg
      241 ctgttggcgg ccggcatggg gctgctcaac ccatcgctca acagcctgat ctcgcgtcag
      301 gcacggatcg acgagcaagg gggcattctc ggtgtatcgc agtcggcgag cagcctcgcc
      361 cgtgctgccg cgccgccgct ggcgggcctg ctgttcgaca cctcggggcg caacacgcct
      421 tacatcgtgg gcgcggccct catgctgctc gtactgatct tggccctccg cctgccgcgc
      481 gtcgcgtctg acgcgacgat gagcgaggta ctgccttcat gaagggtgtc gccgcacccg
      541 tcggcaacaa gccgtccgaa gtcgggctcc gccaggagct gaaggcggtc cgcggcctct
      601 tcccttatct ctggccgcgc gaatgggacc tgcgggcccg ggtcttgctg tcgcttgcct
      661 tcctcgtcgc ggcgaagctg gtcagcgtct acatacccct tctctacaaa ggcgcggtcg
      721 acgcgctctc acccgggcga tccaccgcga tcgtcgtgcc ggtcgcgctg atcgtcttct
      781 atggcctcgg gcgcgttggc gcccagctgt tcgccgaggt gcgcgacgcg gtcttcgcca
      841 aggtcggcca gcgcgcggtg cgccgcgtgg cgctcgctac tttccgccac atgcatgcgc
      901 tctctctccg attccatctg gagcgccaga cgggcgggct gtcgcgcgcc atcgagcgcg
      961 gcatccgcgg caccgaattc ctgctctcct acctgctctt caacgtcgtg ccgacgctct
     1021 tcgagatcct gctggtgtgc ggcattctct ggagcttcta cgacgcgagc ttcgccctgg
     1081 tcacgctggc ggtgatcgtc gcttatatcg tcttcacttt caccgtcacc gactggcgcg
     1141 tgaagttccg ccgcgagatg aacgagcgcg attccgaggc caacacgaag gcgatcgaca
     1201 gtctgctcaa cttcgagacg gtgaaatact tcgccaacga ggagcacgag gcccagcgct
     1261 tcgatagagc gctgcgtgcc tatgagcgcg ctgccgtcaa gagccagacg acgctctccg
     1321 cgctcaatat cggccaaggc gtgatcatcg ccgcgggcct cgtcgtcgtc atgctgctgg
     1381 cggggcgcgg tgtcgcgcgc ggcagcatga ccgtcggcga tttcgtgctg gtgaacgcct
     1441 atctcgttca gctctacgca ccgctcaacg tcctgggcat ggtctatcgc aacatcaagc
     1501 agtcgctcac cgatctcgaa tcgatgttcc gcctgctcgg cgtgcgcgcc gagatcgagg
     1561 atcggccagg cgcgccgccg ctgcgcatcg gccgcggcga gatcgtcttc gatcatgtct
     1621 ccttccgcta cgaccagcgc cgccccatcc tccacgacgt ctccttccgc gtccctccgg
     1681 gtggcaccct cgccatcgtc ggcccgagcg gtgccggcaa atccacgatc gcgcgcctgc
     1741 tcttccgctt ctatgacgtc gactccggag ccatccgcat cgacggccag gatctgcgcg
     1801 cggtgacgca agagagcctg cgccaggcca tcggagtcgt gccgcaggac acggtgctgt
     1861 tcaacgacac catacgctac aacatcgcct acgggcgcac gggcgcgtcc gacgaggaga
     1921 tcgtggccgc ggcgcgccac gcgcggatcg ccgatttcgt ggcgtcgctt ccggacggct
     1981 atcgcagcat ggtcggcgag cgcggcctga agctctccgg cggcgagaag cagcgtgtgg
     2041 ccatcgcccg ggtcatcctc aaggagccga gcatcctcgt cttcgacgaa gcgacctcgg
     2101 ccctcgacac caagaccgag caggagatcc aggcgagtct cgacgaagtg cgctcgcagc
     2161 gcacgacgct ggtgatcgcc catcgcttgt cgaccgtcgt caatgccgac gagattctcg
     2221 tcctcgatga aggccgtatc gtcgagcgcg gcaatcatgc cctcctcctg gcgcagggcg
     2281 gggtctacgc ggcgatgtgg gcgcgccagc aggagttgga tgaacgtgag gctgcgcttg
     2341 ctgccgtggc cgagtgacag cggtggcagc gcaagcgcct tccggagata agcagaccat
     2401 caatctcgcc ctccagggcg ggggcacgca tggcgccttt acttggggcg tgctcgatcg
     2461 cttcctcgag gacgagcgcc tcgccatcga gggcatcagc ggcaccagcg ccggcgcggt
     2521 caatgcggcc gttctcgctc aagggcatgc caaaggtggt tccgccggtg cccgcgccga
     2581 gctcgagcgc ttctggcgcc gcatgagcga gctcggctgg atctcgcccg tgcatcgcgg
     2641 cgtgctcgag cggctgcaag ggacgtggaa cctcgacggc tcgcccagct cgatcctcgt
     2701 cgaccagatg acgcggctcg tgtcgccgta tcaattcaac ccgctcaacc tgtcgccgct
     2761 gcgcgatgtg ctttccagca cgctcgattg gacgacgctg accgcgccgg attccatcaa
     2821 gctcttcatt accgctacca atgtcgagac cggcaagccg cgcgtcttcg cgcgccagga
     2881 tctgacgctc gaggcgctcc tcgcctcgac cgctctgccc cagctcttcc aggcggtggt
     2941 catcgacggc acgccctatt gggacggcgg ctatatgggc aatccgtcca tctggccgct
     3001 gatttatttc tgccaatcgc acgacgtcgt catcgtgcag gtgaatccgc tgacgcgcaa
     3061 aggcgtgccg cggagcgcgg cggagatcac caaccggctc aatgagatca ccttcaacac
     3121 gccgctcatg ggcgagatgc gcgccatctc cttcgtccaa cggctgctgg ccggcgatag
     3181 cgccaatccc gaagtcagcc gcctcaagaa catgctgatc cacatggtcg gggacgagga
     3241 tgccatggag cagctcggcg tcgccagcaa gctcaacacc gatctcgatt tcctgctcta
     3301 tctgagggat ctgggacggc gaaccgccga acgctggctc gacgagaact tcgctgccct
     3361 caaccagcgc tcgagcttcg acgtccggaa ggtgttccta taggacgccg tgccgttcca
     3421 gtacccccgc tttcccgatc catgctatgg agcgtcatgg caaggccggc ggccgcaccg
     3481 aaggagctgg cgacggggct gcgcggcggc gaccggcgag cgctgtcacg cgcgatcacg
     3541 ctggtcgaat cgagtcgcga cgaccaccgc gccgaggccg aggagctcct ggcagagctg
     3601 ctgccggcct cgggcggagc gctccgcctc ggcatttcgg gcgcgccagg cgtgggcaaa
     3661 tcgaccttca tcgaggcgtt cggtctcgcc gccatcggcg cgggccatcg cgtcgccatt
     3721 ctggcggtcg atccgtcgtc gaagcgcggc ggcggctcga tcctcggcga caagacgcgc
     3781 atgccggagc tggcgcgcca tggcgacgcg tatatccgcc cgtcgcccgc cggcaagacg
     3841 ctgggcggcg ttgcccggcg gacgcgggac gccatcacgc tggccgaggc ggcaggtttc
     3901 gacgtcgtca tcgtcgagac cgtcggcgtc ggacaatccg agaccgcggt cgccgacatg
     3961 gtcgacctgt tcctcctgct gctggcgccg ggcggcggcg acgatctcca gggcatcaag
     4021 aaagggatcg tcgagcttgc cgacctgctc gtcgtcaaca aggcggatgg agatcagaaa
     4081 ccggcggcgc tgcgcgccgt cggcgattac cggcatgcgc tgcgcatgct tcgcccggcg
     4141 gcggccgacg aagcgatgcc tctcgtgctc gcggtgtcgg ccctgacggg cgacggcatc
     4201 cctcagatat gggagcgcgt ggaacatcag gccgcgacgc tgctcgccag cggcgagcgc
     4261 gagcgccgac gcgcccggca ggcgcgcgcc gcgctctgga gcgagatcgg ggaggggctc
     4321 gtcgatcttt tcaaggccga tcagcatgtg gccgccaagc ttggcgacat cgaggcccgg
     4381 gtcgccgagg gcacccttac cccctcggcg gcagcccggg cggcgctcgc cgccttccgc
     4441 ggccgggcgc gcgcgacttg acggggatgc ggccggtggc ttagaaaccg tcccttagcg
     4501 cggtcttccg gcaaggaggg ccgtgttctg ctttccgagg atcggtcatg gcgagacgat
     4561 gcatcatcac cggcaagggc gtgcaggtcg gcaacaatgt gagccacgcc aacaataaga
     4621 ccaagcgccg ctttttgccg aaccttcagg tcacctcgct cttgtccgac gcgctcggtg
     4681 ctccgatccg cctgcgtctc tcgacccac
//
LOCUS       DASYNS010000573         3076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5293843, whole genome shotgun sequence.
ACCESSION   DASYNS010000573 DASYNS010000000
VERSION     DASYNS010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5293843"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1304)
                     /gene="queG"
                     /locus_tag="VGP50_17395"
     CDS             complement(183..1304)
                     /gene="queG"
                     /locus_tag="VGP50_17395"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEV8029213.1"
                     /translation="MPPSEIKAAIRSEALALGFDAVGFAPPHLAAEARAHLGEFLARG
                     YHGDMGWLGDRTEQRGDPASLWPEVQSVVVLGLNYAPAEDPLAPLAQKDCGGISVYAR
                     GRDYHDVVKSRLKRLARVIAERWPGDLKVFVDTAPVMEKPLAAMAGVGWQGKHTNLVS
                     RGFGSWLFLGEIYLTLALPPDAAEEDHCGACRRCLDVCPTGAFPAPYRLDARRCISYL
                     TIEHKGHIAAELRPLMGNRIYGCDDCLAVCPWNKFAQAAREPALLSRAGFDAPLLAEL
                     AALDDAAFRALFSGSPVKRIGRDRFLRNVLIAIGNSQEPRLIAAVKPLLPDKSPLVRA
                     MAVWALARLGDRGAIDTLREVHLPKEEDEAVREEWRRAA"
     gene            complement(1304..1768)
                     /gene="queF"
                     /locus_tag="VGP50_17400"
     CDS             complement(1304..1768)
                     /gene="queF"
                     /locus_tag="VGP50_17400"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEV8029214.1"
                     /translation="MSKRRTDELTQLGKHVTPPASPDAAVLEKVANPHPGETYLVRFT
                     CPEFTTLCAVTGQPDFAHLVIDYVPRAWLVESKSLKLYLGSFRNHDAFHEECTLAIAR
                     RLVETLDPQWLRIGGYWYPRGGMPIDVFYQTGAPPEGLWLPDQGVAPYRGRG"
     gene            complement(1775..2902)
                     /gene="tgt"
                     /locus_tag="VGP50_17405"
     CDS             complement(1775..2902)
                     /gene="tgt"
                     /locus_tag="VGP50_17405"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV8029215.1"
                     /translation="MTVFSFSLIARDGAARRGRLATAHGTVETPAFMPVGTAASVKAM
                     RPEDVARTGAEMVLVNTYHVMLRPGAERIEELGGLQRFMNWNGPILTDSGGFQVMSLA
                     KLRKIDENGVTFRSHLDGARHELTPERSIEIQHRLRADVTMAFDECTPFPATPEEAER
                     SMALSMRWAERSKSAYRPRPGHALFGIVQGSVYPELRRRSAETLRGIGFDGYAVGGLA
                     VGEGQAQMFAVLDETVPVLPEDRPHYLMGVGKPGDIIGAVKRGIDLFDCVLPTRSGRT
                     AQAFTRRGTVNLRNARHAADPRPLDPDCACATCTQYSRAYLHHLVRAREILAPMLLTA
                     HNLRYYGDLMSGLRGAIAAGTLDDFATDVAAEQDAGDIPPL"
     gene            complement(2899..>3076)
                     /locus_tag="VGP50_17410"
     CDS             complement(2899..>3076)
                     /locus_tag="VGP50_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEV8029216.1"
                     /translation="AVDLLLTNFHLPRSTLFMLVAAFAGLARMRAAYEHAIASGYRFF
                     SYGDACLLHPEARA"
BASE COUNT          502 a         1021 c         1093 g          460 t
ORIGIN      
        1 tggcattggc gtggatcgtc accgtgcctg cccagagctt ttgcgcgccc gccgtcgcat
       61 aattgatggc ggcggcgcgg ttcatgccgg gcgtctgcgg cgtgttggtg tcgagcgcgc
      121 tgcctttgac gacgcgcacg ccatggctgc gccaatcgat cgtctcgctc atgggggcct
      181 cctcatgccg ctcggcgcca ttcctcgcgc acggcttcgt cttcttcctt cggcaagtgc
      241 acttcgcgca gcgtgtcgat ggcgccgcga tcgccgagac gcgcgagcgc ccacacggcc
      301 atggcgcgca ccagcggcga cttgtccggg agcagcggct tcaccgccgc gatcaggcgc
      361 ggctcttggc tgttgccgat ggcgatgagg acgttgcgca ggaagcggtc gcggccgatg
      421 cgcttcaccg gcgagcccga gaagagcgcg cggaacgcgg cgtcgtcgag cgccgcgagc
      481 tccgccagaa gcggcgcgtc gaagccggcg cgggaaagga gcgcgggctc gcgcgcggct
      541 tgcgcgaatt tgttccaggg gcagacggcg aggcaatcat cgcagccata gatgcggttg
      601 cccatgagcg gccggagctc ggcggcgatg tgtcccttgt gctcgatcgt gagataggag
      661 atgcagcgcc gcgcatcgag gcgatagggc gcgggaaagg cgccggtcgg gcagacatcg
      721 agacagcgcc ggcaggcgcc gcaatgatcc tcttccgccg catcgggcgg gagcgccagc
      781 gtcagataga tctcgccgag aaacagccag gagccgaaac cgcgcgagac caggttggtg
      841 tgcttgcctt gccagccgac acccgccatc gcggcgagcg gcttctccat caccggcgcc
      901 gtgtcgacga aaactttgag gtcgcccggc cagcgctcgg cgatgacgcg cgcgagccgc
      961 ttcaagcgcg acttgacgac gtcatggtag tcgcgcccgc gcgcatagac ggagatcccg
     1021 ccgcagtctt tctgcgcgag cggtgccagc ggatcttctg ccggcgcata gttgaggccg
     1081 agcacgacga cgctttgcac ctcgggccaa agcgacgcgg ggtcgccgcg ctgctccgtg
     1141 cgatcaccga gccagcccat gtcgccgtga tatccccgcg ccaggaactc gccgagatgc
     1201 gcccgcgctt ccgccgcaag atgcggcggt gcgaacccca cggcgtcgaa gcccagcgcc
     1261 agcgcctcgc tgcggatggc ggccttgatc tcgctgggcg gcatcagccg cgtccgcgat
     1321 agggcgccac cccttggtcc ggcagccaca ggccttcggg cggcgcgccc gtctggtaga
     1381 agacgtcgat gggcatcccg ccccgcggat accagtagcc gccgatgcgc agccattgcg
     1441 gatcgagcgt ctcgaccagg cggcgggcga tggcgagggt gcactcctcg tggaaggcgt
     1501 catgattgcg gaacgagccg agatagagct tgagcgactt gctctcgacg agccaggcgc
     1561 gcggcacata gtcgatgacg agatgcgcga aatccggctg ccccgtgacg gcgcagagcg
     1621 tggtgaactc gggacaggtg aagcgcacga gataggtctc gccgggatgc gggttcgcca
     1681 ctttctccag cacggccgcg tcgggcgagg cgggcggtgt cacgtgcttg ccgagctgcg
     1741 tgagctcgtc ggtgcggcgc ttgctcatgc tcactcacaa cggtggaatg tcgccggcgt
     1801 cctgctcggc cgcgacatcg gtggcgaagt cgtcgagcgt gccggcggcg atggcgccgc
     1861 gcaggccgct catgaggtcg ccataataac ggagattgtg cgccgtcaac agcatgggcg
     1921 cgaggatctc gcgtgcgcgc acgagatgat ggagataggc ccggctgtat tgcgtgcaag
     1981 tggcgcaggc gcaatcggga tcgagcggcc gcggatcggc cgcgtgccgc gcattgcgca
     2041 gattgaccgt gccgcgccgc gtgaaggcct gcgccgtgcg tcccgagcgc gtcggcagca
     2101 cgcagtcgaa gagatcgatg ccgcgcttca cggcgccgat gatgtcgccg ggcttgccga
     2161 cgcccatgag gtaatgcggc cggtcctcgg gcaggaccgg caccgtctcg tcgagcacgg
     2221 cgaacatctg cgcctgccct tcgccgacgg cgagcccgcc caccgcatag ccgtcgaagc
     2281 cgatgccgcg cagcgtctcc gccgaacgcc ggcgcagctc gggataaacg ctgccctgga
     2341 cgatgccgaa gagcgcgtga ccagggcgcg gccgataggc ggatttcgag cgctcggccc
     2401 agcgcatgga cagcgccatc gagcgctcgg cctcttcggg cgtcgccggg aagggcgtgc
     2461 actcgtcgaa ggccatggtg acgtcggcgc ggaggcggtg ctggatctcg atcgagcgct
     2521 cgggcgtgag ctcgtggcgc gcgccgtcga gatgcgaacg gaacgtgacg ccattttcgt
     2581 cgattttgcg cagcttcgcc agcgacatga cctgaaaccc gccggaatcc gtgagaatcg
     2641 gcccgttcca gttcataaaa cgctgcaatc cgccgagctc ttcgatcctc tccgcgcccg
     2701 gccgcagcat cacatggtag gtattgacga gaaccatctc ggccccggtc cgagcgacgt
     2761 cctcgggccg catcgccttg acgctcgccg ccgtgccgac cggcatgaaa gccggcgtct
     2821 cgaccgtgcc atgcgccgtc gcgagacgcc cgcgccgcgc cgcgccgtcc cgcgcgatga
     2881 gcgaaaagga aaacaccgtc acgcccgcgc ctcgggatgg agcaggcacg catcgccgta
     2941 agagaagaag cgatagcccg aggcgatggc gtgctcgtaa gcggcgcgca tccgcgcgag
     3001 cccggcgaag gcggcgacga gcatgaacag cgtcgagcgc ggcagatgga aattggtgag
     3061 cagcagatcg acggcg
//
LOCUS       DASYNS010000574         8614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1237071, whole genome shotgun sequence.
ACCESSION   DASYNS010000574 DASYNS010000000
VERSION     DASYNS010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237071"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VGP50_17415"
     CDS             complement(<1..255)
                     /locus_tag="VGP50_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8029217.1"
                     /translation="MIEPQRAQPTEALSIVDVRSGTLQVRLAETMADIDAAQALRYRV
                     FYEEMGASPSAEMARIERDFDSFDGVCDHLLVVDHARGSGA"
     gene            complement(278..703)
                     /locus_tag="VGP50_17420"
     CDS             complement(278..703)
                     /locus_tag="VGP50_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEV8029218.1"
                     /translation="MPSRLEQLCLDKGLKMTEQRRVIARVLSDSADHPDVEALHRRAT
                     EIDPKISIATVYRTVRLFEEASILARHDFGDGRARYEEMPTDHHDHLIDLQTGRVIEF
                     RNEDIEKLQRDVAERLGYRLVDHRLELFAVPLAGKAERK"
     gene            complement(809..1228)
                     /locus_tag="VGP50_17425"
     CDS             complement(809..1228)
                     /locus_tag="VGP50_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEV8029219.1"
                     /translation="MTENTNSNDLLALTTEIVAAHVSNNTVAVSDLPQLINQVYQSLS
                     SIGKNPATTAERPQPAVSVKRSVHPDYIICLEDGKKLKMLKRHLKTAYDMTPDEYRER
                     WSLPPDYPMVAPNYARQRSKLAKEIGLGTRGRRTNAA"
     gene            complement(1450..1911)
                     /locus_tag="VGP50_17430"
     CDS             complement(1450..1911)
                     /locus_tag="VGP50_17430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV8029220.1"
                     /translation="MTRRLRSVQPQDLNRLARLHAQCFPEERWDARALTELLAMKGAS
                     GHLLEDGPSALPQGLILDLFQAEDAEILTLCVAPPARRQGIGRLLLEDLFRRAILLGA
                     RSVSLEVAADNHAARRLYEALGFAPAGHRSGYYRRGRTTIDALLFRRPLAL"
     gene            complement(1908..2591)
                     /gene="tsaB"
                     /locus_tag="VGP50_17435"
     CDS             complement(1908..2591)
                     /gene="tsaB"
                     /locus_tag="VGP50_17435"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV8029221.1"
                     /translation="MVSVLAFDSAGHGCSAAVLHRGRVASRRSAAMERGQAEALMPMI
                     EAVLAESDLVSGDLDLIAVTVGPGSFTGLRIALATAQGLALATGRPLLGITNFAAMAA
                     QVRAPARSGRRLVVALDSKRKELYLQSFDLEGVADGEGALVAPEAFATWVPEGPLIVA
                     GDGAPRLAATLGEPRAWLTPGSGMVDAADVARLAAATWRPGVRPPRPRPLYLRAPDTT
                     TPHPGLARP"
     gene            2706..3257
                     /gene="ddpX"
                     /locus_tag="VGP50_17440"
     CDS             2706..3257
                     /gene="ddpX"
                     /locus_tag="VGP50_17440"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HEV8029222.1"
                     /translation="MSLIEITPESHDLVIDLAYATSRNFTGRPIYRRAACYLHSDAAA
                     RLSRAVELGRALGFRFKLLDALRPSEAQWLLWNACPDPNFLADPRRGSPHSRGVAVDL
                     VPLDGEGRELEMGTAFDAFTPLSHHGNTEIAPAAQRNRFLLLGLMSAAGWDFYANEWW
                     HYQLFDPKRYPLLSDADLPTPMM"
     gene            complement(3272..3793)
                     /locus_tag="VGP50_17445"
     CDS             complement(3272..3793)
                     /locus_tag="VGP50_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV8029223.1"
                     /translation="MTRILDDRSLDSLFRAFLPCRNWLDKPVSDAIIEAVWELAKLPP
                     TASGVYPARIVFVRSKAAKGRLLPALAVADRVGSAAAPITAILGYEPAGPSADGWRDS
                     TLQAGYLMVAARALGLDCAPLTLADPALVDREFFTDGRAKSNFACNLGYGHPDAPDRR
                     ADGPAFEEACRTI"
     gene            complement(3818..4363)
                     /locus_tag="VGP50_17450"
     CDS             complement(3818..4363)
                     /locus_tag="VGP50_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEV8029224.1"
                     /translation="MFIQTEPTPNPATLKFLPGRPVIEQGTANFTDATAAARSPLAQR
                     LFALPGVSGVFLGADFITVSKDQEADWFELKAVVLGAIMEHFTTGAPVILGGGESAPA
                     DEDEDEVVQQIKELLETRVRPAVAQDGGDILFHSYEDGVVYLHMQGSCSGCPSSTATL
                     KAGIENMLRHYVPEVQEVRAV"
     gene            complement(4481..4957)
                     /locus_tag="VGP50_17455"
     CDS             complement(4481..4957)
                     /locus_tag="VGP50_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV8029225.1"
                     /translation="MDETAAPRIFLVVVDDSEEMRVALRYAALRAKHTGGRVALLYVI
                     EPSDLQQWMAVETLMREERREQAEALLQKLTAEVSDLCGAVPIVHIREGRRGDELLAL
                     LAEEPSISILVLAAGAGPEGPGPLVTHLVGKMSGRMRVPITVVPGNLSDEQIAALS"
     gene            complement(4965..5705)
                     /locus_tag="VGP50_17460"
     CDS             complement(4965..5705)
                     /locus_tag="VGP50_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014249245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HEV8029226.1"
                     /translation="MRRWLAALLLWAVLAAPARAAEPLIADITTHFIAITTGFTGANI
                     VLFGATDGTGDIVVVVSGPEHAVVVRRKSRVAGLWINTREAAFSGVPSFYVVYSSRPL
                     DDIAPPPMQARLQIGLANLPFEPTGRRRPAAELAEFRAALIRERQREGLFGEGAGRVY
                     FLGDRLFRADIALPANLPTGEYSVRIYQVKDGAEVAAQTSPLFVSQAGLDADVFDFAD
                     RHALLYGIVAVAGAAMAGWLASLPFRNA"
     gene            complement(5702..6619)
                     /locus_tag="VGP50_17465"
     CDS             complement(5702..6619)
                     /locus_tag="VGP50_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV8029227.1"
                     /translation="MDIYLPIAAISLDVFVLLGLGTAVGFLSGVFGVGGGFLLTPFLI
                     FIGVPPAVAVASSANQLVGASVSGALAHWRRGHVDVQMAMVLLAGGFAGSALGVWIFA
                     LLRRIGQAELAITLSYTLLLGTLGALMLAESTRAMLRLRRPGVSRRKLHQHIWLHGLP
                     LKMRFRRSKLYISALLPIGLGFAVGMLSAIMGIGGGFVMVPAMIYILGMPTATVPGTS
                     LFQIIFVAASVTILQAIENGTVDAVLALVLLVGGVIGAQLGTRFGQRLRGEQLRALLA
                     LLVLAVAAKLLFDLTVPPDDIYSLGTLVP"
     gene            6780..7565
                     /locus_tag="VGP50_17470"
     CDS             6780..7565
                     /locus_tag="VGP50_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8029228.1"
                     /translation="MTDPFDFSGKNVFVAGGSSGINLGIAEAFAAAGANLAIASRSQE
                     RVDAACASLRRHGGGVLGKSADVRDYAAIAAALADAALEFGPIDVLVSGAAGNFPALA
                     LGMSANAFKAVVDIDLLGTFNVLRAAHQHLRKPGAAIINISAPQSYKPMALQVHACAA
                     KAGVDQVTRVLAIEWGEDGIRVNSIAPGPIEDTEGFRRLGTPEGHALMKKRLPLRRFG
                     TLEDVARLALFLASPLASYITGTLIPVDGGGSLLGAGLSSRTA"
     gene            7638..8531
                     /locus_tag="VGP50_17475"
     CDS             7638..8531
                     /locus_tag="VGP50_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029229.1"
                     /translation="MASRPLRVLLQTTIQPIADDWHIGRFGLLRQHLADLKEKEGHPL
                     CAVVARDRATPPGSDDPVLATIDRSDFDELWLFAVDGGNGLTPAECRAIDAFRRRGGG
                     LMVTRDHMDLGSSVTALAEIGAAHYFHSRNLDPDSERRRIDDPFTTHIAWPNYHSGAN
                     GDHQVIEAVDPDHPLLRNGAAAGGTIRYFPAHPHEGGIGVPAGAGDARIVARGRSKVT
                     GALFNLVVAFDPAVEARGRAVAQSTFHHFCDYNWDVAAGCPSFVDEPPGTGMSSRPEG
                     RRDIETYVRNLVLWLARVPVP"
BASE COUNT         1414 a         2888 c         2903 g         1409 t
ORIGIN      
        1 tgctccgctg ccgcgcgcat gatcgaccac gaggagatgg tcgcagacgc catcgaagct
       61 gtcgaagtcg cgctcgatgc gcgccatctc ggcacttggg ctcgccccca tctcttcata
      121 gaagacccgg tagcgcaggg cctgcgccgc gtcgatgtcg gccatcgtct cggcgagccg
      181 gacctgaagc gtgcccgagc ggacatcgac gatcgacagc gcttcggtcg gttgggcgcg
      241 ctgcggctcg atcacggcgt cccgcggcgg caagcgatca tttgcgctcg gccttgccgg
      301 ccagcggcac ggcgaagagt tcgaggcggt gatcgaccag tcggtagccc agccgctcgg
      361 cgacgtcgcg ctgcagcttc tcgatatcct cgttgcggaa ctcgatgacg cggccggtct
      421 ggagatcgat gagatggtcg tggtggtccg tcggcatctc ctcgtagcgc gcccggccgt
      481 cgccgaaatc atggcgcgcc aggatgcttg cctcctcgaa gagccgcact gtgcgataga
      541 ccgtggcgat gctgattttg gggtcgatct cggtcgcgcg gcgatgcaag gcctcgacat
      601 cggggtggtc cgccgagtct gagagcaccc gcgcaatcac gcggcgctgc tccgtcatct
      661 tcagcccctt gtcaaggcaa agctgctcta gtcgggaggg catccccacc tgtcgcattg
      721 acgccaccaa agcagtatag ctcaaaggtg cgggcgatcc cataccgcgc gctcatggcg
      781 ccacgggcat gtctgcgcgg agcgcgtctc acgcagcgtt ggttcgccgt ccgcgcgtgc
      841 cgagcccgat ttccttggcg agcttgctgc gctggcgcgc atagttgggc gccaccatcg
      901 ggtagtcggg cggcaggctc cagcgctcgc gatattcgtc gggcgtcatg tcataagctg
      961 tcttcaggtg gcgcttgagc atcttgagct tcttgccatc ctcgaggcag atgatgtagt
     1021 cgggatgcac cgagcgcttg acgctgaccg cgggctgagg ccgctccgcg gtcgttgccg
     1081 gattcttgcc gatgctggaa agggattggt aaacttggtt gattagctgc ggcaagtcac
     1141 tcaccgcgac cgtattgttt gatacatgtg ccgcaacaat ttcagtcgta agggccagca
     1201 gatcgtttga gttggtattt tcagtcatcg ggcacaattc ctctatgcag gcacttgcac
     1261 cgatatatgt gtttttctgc aacgaagcaa tgaccatcac gtacgcaaga ttactttttt
     1321 tgacggtctg atgaacggcc tgccggggca tgaaaaggcg ggatgttgac ccacgcggac
     1381 ttgccgtgtg cgcccacggt caagtccacc ctcagtcaag cagccgcgcg gccaagttgc
     1441 catcttgtgt caaagtgcca gcgggcggcg aaacagcagg gcgtcgatcg tcgtccggcc
     1501 gcgccggtaa tagccggaac ggtgcccggc cggtgcaaaa cccagagcct cataaagccg
     1561 tcttgcggca tggttgtcgg ccgccacttc gaggctgacg ctccgcgcgc ccaaaagaat
     1621 ggcacggcgg aacaggtctt cgagcagaag tcgcccgatt ccctgccggc gcgccggtgg
     1681 cgcgacgcag agggtgagga tctccgcgtc ttcggcctgg aacagatcga gtatgagccc
     1741 ctgcggcaga gccgacggtc cgtcctcgag gagatggccg ctggcgccct tcatggcgag
     1801 cagctccgtc agcgcacgag cgtcccagcg ttcttcgggg aagcactgcg catggagccg
     1861 cgccaagcgg ttgagatcct ggggctgaac cgatcgcaga cggcgcgtca tggccgggcg
     1921 agcccgggat gtggcgtcgt ggtgtcgggg gcgcgaagat agagaggccg cggcctcggc
     1981 ggccggacgc cgggtcgcca ggtcgctgcc gccagccgcg ccacatcggc ggcgtcgacc
     2041 atgccggagc cgggtgtgag ccacgcccgc ggctcgccga gcgtggctgc gagacgcggc
     2101 gcgccgtcgc cggcgacgat cagcgggcct tccgggaccc aagttgcgaa cgcttccggt
     2161 gcgaccaagg caccctcgcc gtctgccacc ccctcgaggt cgaaggactg gaggtagagc
     2221 tcttttcgct tgctgtcgag ggcgacgacc agccttcgcc cgctcctcgc cggcgcccga
     2281 acctgcgcgg ccatggcggc gaaattggtg atgccaagga gcgggcgccc ggtggcgagg
     2341 gccaagccct gggctgtcgc cagcgcgatg cgcagcccgg tgaaggagcc ggggccgacc
     2401 gtcaccgcga tcaggtcgag atcgccgctc accagatcag actcggcgag cacggcctcg
     2461 atcatcggca tcagcgcctc ggcctgacca cgctccatgg cggccgaacg ccgcgacgcc
     2521 acgcggccgc gatgcagcac cgccgcggag catccatggc ccgcgctgtc gaaagcgagg
     2581 acgctcacca tccgggcgct tcccggcgcc cgggacggcg gccacccata tctgttgcgg
     2641 ccgggccctg gcggcgggta aaggaaggca tcttgcgtct cctcccagag gcaaccctgc
     2701 ccgcgatgtc gctcatcgag atcacacccg aaagccacga tctcgtcatc gatctcgcct
     2761 atgcgacgtc gcgcaatttc accgggcgtc ctatctatcg gcgcgcggca tgctatctac
     2821 atagcgatgc ggcggcgagg ctctcaagag cggtggagct gggccgcgcg ctcggctttc
     2881 gcttcaagct cctcgacgcg ttgcgcccct cagaagcgca atggttgcta tggaatgcct
     2941 gtcccgaccc gaatttcctc gcggatccgc gccgcggctc gccgcattcg cggggcgtcg
     3001 ccgtcgatct cgtcccgctc gatggcgagg gtcgtgagct cgagatgggc accgctttcg
     3061 acgccttcac gccgctctcc catcacggca acacggagat cgcgcctgcc gcacagcgca
     3121 accgctttct tctgctcggc ctcatgagcg cggccggctg ggacttctac gccaacgaat
     3181 ggtggcacta ccagctgttc gatcccaaac gctatccgct gctgtccgac gccgatctgc
     3241 cgacgccgat gatgtgacgc gcgggccgcg ctcagatggt acggcaggct tcttcgaagg
     3301 cgggcccgtc ggcacgccgg tcgggtgcgt cgggatggcc ataacccaga ttgcaggcga
     3361 aattgctctt ggcgcggccg tcggtgaaga actcgcggtc gaccaacgcc gggtcggcaa
     3421 gggtcagcgg tgcgcagtcg agacccaaag cgcgggcggc gaccatgaga taaccggcct
     3481 gcaaagtgct atcgcgccag ccatccgccg atggcccggc cggctcgtag ccgagaatgg
     3541 cggtaatcgg cgcggcggcg ctgccgacgc ggtcggcaac ggccagcgcc ggaagaaggc
     3601 gacccttcgc cgccttcgat cggacgaaaa cgatgcgggc cggatagacg ccgctggcgg
     3661 tgggcggcag cttggccagc tcccagaccg cctcgatgat ggcgtcgctc accggcttgt
     3721 cgagccagtt ccggcagggg agaaaggcgc ggaaaaggct gtcgaggctc cggtcatcga
     3781 ggatccgcgt catttgctcc ggtccccggc gcgcggctca gacggctctc acttcctgca
     3841 cctcgggtac atagtggcgc agcatgttct cgatgccggc cttcaaggtc gccgtcgagc
     3901 ttgggcagcc cgagcacgag ccctgcatgt gcagatagac gacgccgtcc tcgtagctat
     3961 ggaaaaggat gtcgccgcca tcctgagcca ccgccggacg cacccgcgtc tcgagcagct
     4021 ccttgatctg ctgtaccacc tcgtcctcgt cctcgtccgc gggcgcgctt tcgccgccgc
     4081 cgaggatgac cggcgcgccg gtggtgaagt gctccatgat cgcgcccagc acgaccgcct
     4141 tgagctcgaa ccagtcggct tcctggtcct tgctgaccgt gatgaaatcc gcgccgagga
     4201 agacgccgct gacacccgga agcgcaaaga gccgctgcgc cagcggcgac cgtgccgccg
     4261 ccgtggcgtc ggtgaaattg gccgtgccct gctcgatgac ggggcgtccc ggcaggaatt
     4321 tgagggtggc ggggttgggc gtgggctctg tctggatgaa catcgcgtcc tctggcgtct
     4381 tggatttgag ccgatgtctt gccgctctag cttggatcgc cggcccctca agtcaaccct
     4441 ttggcgtgta aggttaaggc gctgtgcccg ccgcctcggc ttagctcagc gccgcgatct
     4501 gctcgtcgct gaggttgccc ggcaccaccg tgatcggcac gcgcatgcgg cccgacatct
     4561 tgccgaccag atgggtgacg agcgggcccg gtccctcggg cccggcgccc gcggccagca
     4621 ccaggatgga gatgctcggc tcctccgcga gcaacgccaa gagctcgtcg ccgcggcggc
     4681 cttcgcggat atggacgatg gggacggcgc cgcagaggtc gcttacttcc gccgtgagct
     4741 tctgcagcag ggcttccgcc tgctcgcgcc gctcctcgcg catcagcgtc tcgaccgcca
     4801 tccattgctg caagtcgctc ggctcgatga catagaggag cgcgacgcgt ccgcccgtat
     4861 gcttcgcccg caaggccgcg tagcgcaaag cgacgcgcat ctcctcgctg tcgtcgacga
     4921 cgaccaggaa gatgcgtggt gcggccgttt cgtccatcgc tgcctcaggc gttgcggaag
     4981 ggcaggctcg cgagccatcc tgccattgcc gcgccggcca cggcaacgat gccgtaaagc
     5041 agcgcatggc gatcggcgaa gtcgaagacg tcggcgtcga ggccggcttg cgagacgaag
     5101 agcggcgaag tctgcgccgc gacctcggca ccgtccttca cctggtagat tcggacgctg
     5161 tactcgcccg tcggcaggtt ggcgggcaac gctatgtcgg cgcggaacag gcgatcgccc
     5221 aggaaataga cgcggccggc accttcgccg aagagcccct cgcgctggcg ctcgcgaatg
     5281 agcgccgcac ggaactcggc cagctcggcc gccgggcggc gcctgccggt cggctcgaac
     5341 ggcagattgg cgaggccgat ctgaagtcgc gcttgcatcg gcggcggcgc gatgtcgtcg
     5401 agcggccggc tgctatagac aacgtaaaag ctcggcacgc cggagaaggc cgcctcgcgg
     5461 gtgttgatcc agagtccggc gacgcggctt ttgcgccgca cgaccacggc gtgctcgggc
     5521 ccgctcacca cgacgacgat gtcgccggtg ccgtccgtcg cgccgaacag cacgatattc
     5581 gcgccggtaa accccgtggt gatggcgatg aaatgcgtgg tgatgtcggc gatgagcggc
     5641 tcggccgcgc gggccggcgc cgcgagcacc gcccagagca acaatgccgc gagccagcgt
     5701 ctcatggcac cagtgtcccc agcgagtaga tgtcgtcggg cggtaccgtc aggtcgaaga
     5761 ggagcttggc ggccacggca agcacgagga gggccaagag cgcgcgcagc tgctcgccgc
     5821 gcaacctctg gccgaaccgc gtgccgagct gcgcaccgat gacgccgccc accagcagca
     5881 ccagcgccag cacggcatcg acggtgccgt tctcgatcgc ctgcaggatg gtgacggagg
     5941 ccgcgacgaa gatgatctgg aagagcgagg ttcccggcac cgtcgcggtt ggcatgccga
     6001 ggatatagat catcgccggg accatcacga agccgccacc gatgcccatg atggccgaga
     6061 gcatgccgac ggcgaagccg agcccgatcg ggagaagcgc gctgatgtag agcttcgagc
     6121 ggcggaagcg catcttgagc ggcaagccat gcagccagat gtgctggtgg agcttgcgcc
     6181 gcgacacgcc ggggcgccgg aggcgcagca tggcacgcgt gctctcggcc agcatcagcg
     6241 cgccgagcgt gcctagcagc agcgtgtagg agagggtgat ggccagttcg gcctggccga
     6301 tccgccgcag cagcgcgaag atccagacac ccaaggccga gcccgcgaag ccgccagcca
     6361 gcagcaccat cgccatctgc acgtcgacat ggccgcgccg ccaatgggcg agcgcacccg
     6421 aaacggacgc tccgaccagc tggttcgctg acgaggcgac tgcgacggcg ggcggcacgc
     6481 cgatgaagat gaggaacggc gtcagcagga atccgccgcc gacgccgaag acgcccgaga
     6541 ggaagcccac cgcagtgccc agccccagca ggacgaagac gtccagcgag atcgcggcaa
     6601 tcggcaggta gatgtccatt ggcgggacgg atttgttctt gaaagcgatg cgccgcgggc
     6661 agggttgcca gcttacaacc gcgccgcacg ccgggcaaag tgctttcgcc ccgtgcctcg
     6721 gcgatgcctc agcgtcgcgc ccatgtccta gaatggcgcg cggcacagag gaggaggtca
     6781 tgaccgatcc attcgatttt tcgggcaaaa acgtattcgt cgccggcggg tcgagcggca
     6841 tcaatctcgg catcgccgag gcctttgccg ctgccggcgc caatcttgcc atcgccagcc
     6901 gctcccaaga gcgggtcgat gccgcctgtg cgtcgctgcg gcgccatggc gggggcgtgc
     6961 tggggaagag cgccgatgtg cgcgactatg ccgcaatcgc cgcggcactt gcggatgccg
     7021 cgcttgagtt cggtccgatc gacgtcctgg tctcgggtgc cgcgggcaat ttcccggcgt
     7081 tggcgctcgg catgtccgcc aacgccttca aggcggtggt cgatatcgac ctgctcggca
     7141 ccttcaacgt gctgcgcgcc gcgcatcagc atctgcgcaa gcccggcgcg gcgatcatca
     7201 acatctcggc gccgcaatcc tacaaaccga tggcgctcca agtgcatgcc tgtgccgcca
     7261 aggccggtgt cgaccaggtg acgcgcgtcc tggcgatcga atggggcgag gacggcatcc
     7321 gcgtcaactc gatcgcgccc ggcccgatcg aggataccga gggtttccgc cggctcggca
     7381 cgcctgaagg gcatgcgttg atgaagaagc gcctgccgct ccggcgcttc gggaccctcg
     7441 aggacgtcgc ccggctcgcg ctctttctcg cctcgccgct cgcgagctac atcaccggca
     7501 cgctcattcc ggtggatggc ggcgggtcgc ttctcggcgc cggcctcagc tcgaggacgg
     7561 cctagccgaa agaccaggtg tcgctcggcg ccgctcgtac cagattggcg gcaagtcggg
     7621 tcgtaaagga ggagagaatg gcgtcgcggc cgttgcgcgt gctgctgcag accacgattc
     7681 agccgatcgc cgatgattgg catatcggtc gcttcggcct tctccggcag catctcgccg
     7741 atcttaaaga gaaggaaggg catccgctct gcgcggtggt ggcacgcgac cgcgcgacgc
     7801 cgccgggcag cgacgatccg gtgttggcca cgatcgaccg ctccgacttc gacgagctct
     7861 ggcttttcgc cgtggacggt ggcaacggcc tgacgcccgc cgagtgtcga gcgatcgatg
     7921 cgttccgccg gcgcggcggc ggcctcatgg tgacgcgcga ccatatggat ctcggatcgt
     7981 ccgtgaccgc gctcgccgaa atcggcgcgg cgcattactt ccacagccgc aacctcgatc
     8041 ccgattccga gcgccggcgg atcgacgatc ccttcacgac gcatatcgca tggcccaact
     8101 accactccgg cgccaatggc gaccatcagg tgatcgaggc cgtcgatccg gaccacccgc
     8161 tgctgcgcaa tggcgccgcc gccggcggga ccatccgcta ttttccagca catccacacg
     8221 agggcggtat cggcgtcccc gccggcgccg gggacgcgcg gatcgtcgcg cggggccgga
     8281 gcaaagtgac cggcgctttg ttcaacctgg tcgtcgcctt cgatcccgcc gtcgaagcgc
     8341 gcggccgcgc ggtcgcccaa tccaccttcc atcatttctg cgattacaat tgggacgttg
     8401 ccgccggctg cccgagcttc gtcgacgagc cgccgggtac tggtatgtcg agccgccccg
     8461 aagggcggcg cgacatcgaa acctatgtcc gcaatctcgt gctctggctt gcgcgcgtgc
     8521 cggtcccgtg acgcgcgtca gggccgcagc agccccatga tgtcgaagac ctgacgcagg
     8581 ttgggctcgg cgatggcgcg ggcgcgctcg ctgc
//
LOCUS       DASYNS010000575         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_616627, whole genome shotgun sequence.
ACCESSION   DASYNS010000575 DASYNS010000000
VERSION     DASYNS010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616627"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /gene="hemB"
                     /locus_tag="VGP50_17480"
     CDS             complement(<1..880)
                     /gene="hemB"
                     /locus_tag="VGP50_17480"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEV8029230.1"
                     /translation="MVREVVLTADDLIWPLFLLDGTKVRAGVDSMPDVERLSVDEAVR
                     AAERAAKLAIPCIALFPHTDLKQRDEAGSEALNPDNLVCRAIRAIKKEVPEIGVLCDV
                     ALDPYTSHGHDGLMRDGVILNDETVAVLVRQALVQAEAGCDIIAPSDMMDGRVGAIRD
                     GLDAAGFGDVQIMAYAAKYASAFYSPFRDAVGSNATLTGDKRTYQMDPANTDEALREV
                     ELDIAEGADMVMVKPGLPYLDVLYRVKEAFGMPTFAYQVSGEYAMIMAAAGNGWLDGE
                     KAMTESLIAFKRAGADG"
     gene            1165..>1622
                     /locus_tag="VGP50_17485"
     CDS             1165..>1622
                     /locus_tag="VGP50_17485"
                     /inference="COORDINATES: protein motif:HMM:NF039363.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6163 family protein"
                     /protein_id="HEV8029231.1"
                     /translation="MTDQTQPRPRQPGEMLEPVRASGKSPDPVGAWTRYLILFLRVMA
                     AIWLVKGLYHWAAVCGVGVDADGGFEAHTLAWQTATVLFAILDLVAAVGLWLAAPWGA
                     VVWLTSVVSMAAVDLFFPQVYGGSLSMALGVLGLLGIYLLLAIFAARERPA"
BASE COUNT          227 a          529 c          569 g          297 t
ORIGIN      
        1 ccccgtcggc gccggcgcgc ttgaaggcga tcaggctctc ggtcatggcc ttctccccat
       61 cgagccagcc attgccggcg gcggccatga tcatcgcata ttcgccggag acttggtagg
      121 caaaggtggg catcccgaag gcttccttga cccggtagag aacgtccaaa taaggaagtc
      181 ccggcttgac catcaccatg tcggcgcctt cggcgatgtc gagctcgacc tcacgcagcg
      241 cctcgtcggt gttggccggg tccatctgat aggtccgctt gtcgccggtg agggtcgcgt
      301 tcgagccgac cgcgtcgcgg aacgggctat aaaaagccga ggcgtatttg gccgcataag
      361 ccatgatctg cacgtcgccg aagccggcgg cgtcgagccc gtcgcggatc gcgccgacgc
      421 ggccgtccat catgtccgac ggcgcgatga tgtcgcagcc ggcttcggcc tgcaccaggg
      481 cctggcgcac cagcaccgcg acggtctcgt cgttgaggat gacgccgtcg cgcatcaggc
      541 cgtcatggcc gtggctggta tagggatcga gggcaacgtc gcagaggacg ccgatttccg
      601 gcacctcttt tttgatggcg cggatcgcgc ggcagacgag attgtcgggg ttgagcgctt
      661 ccgagccggc ctcgtcgcgc tgtttcaggt cggtatgggg aaaaagcgcg atgcagggga
      721 tggcgagctt ggccgcccgc tcggcggcgc gcactgcctc gtcgaccgaa aggcgctcca
      781 cgtcaggcat cgagtcgacg cccgcgcgga ccttggtccc gtcgaggagg aacaacggcc
      841 agatcaggtc gtcggcggtg agcacgacct cgcgcaccat gcgccgcgcc cattcggcgc
      901 ggcggttgcg gcgcgggcgg atggcgaggt cgaggcgctc ggccggagcc tcgggcgcat
      961 gcggcagcgg ggcagaggcg ggcgcgaggc ggccgtgttc cagcggccgg ccgaacttga
     1021 tcgccattgg cggctccttg ttggcagcgg ctctttttac ccgttccagc cttgtcccgc
     1081 caccttgtcg gagcacgcca aacaagccgg cgcttgcggc tggccgattg actgtgccgg
     1141 cctcgaatgc caaacgcgcc tgccatgacc gatcagaccc agccgcgacc ccgccagccc
     1201 ggcgagatgc tcgaaccggt gcgtgcctcc ggcaaaagcc ccgatccggt gggagcttgg
     1261 acccggtatc tcattctgtt tctgcgcgtc atggccgcga tctggctggt caagggcctc
     1321 taccattggg cggcggtgtg cggcgtcggc gtggacgcgg atggcgggtt cgaagcgcat
     1381 acgctcgcct ggcagacggc gacggtgctc ttcgccatcc tcgatcttgt cgccgcggtc
     1441 gggctgtggc tggcggcgcc ttggggcgcg gtggtgtggc tcacctccgt tgtctccatg
     1501 gccgcggtcg atctgttctt tccgcaggtc tatggcggca gcctctcgat ggcgctcggc
     1561 gtgctcggat tgctcggcat ctatttgttg ctcgcgatct tcgccgcgcg cgagcggccg
     1621 gc
//
LOCUS       DASYNS010000576         9541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3270464, whole genome shotgun sequence.
ACCESSION   DASYNS010000576 DASYNS010000000
VERSION     DASYNS010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9541
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3270464"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1904
                     /locus_tag="VGP50_17490"
     CDS             270..1904
                     /locus_tag="VGP50_17490"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEV8029232.1"
                     /translation="MRKSWRDGGYRWQRLALLAATAFRHGAPLAFDLLRGRPVRQEDV
                     ARRLRAALEALGLTYLKLGQFLATRHDLVPAAVVRELTALFEDVPALPAATVAGIVAR
                     ELGAARDGLFASFDPVPVAAATVAQVHRAELTDGSSVAVKVQRPGIEALFEADISILR
                     SLARLGDRLGLLGSLSAVAIADQFAAYTRREFDFLLEASTAERLRREARPECIVPRIV
                     PELTTRRVLTMQFMEGVSLARICTLVESGDEATLRAVLPSFDRRRVLENLTLVSLHQF
                     FVTGFFHGDPHPGNILVCPDNRVVLLDFGIFGELGAAGRDLLASYAEHLAMGNVAESF
                     RHLSQIYFPGPRSDRRAFRQDAIAALRRWYVASRDPSTPMRERHLGGSFDAMVGVVRR
                     HRYWTTMDYLLFWRAIIVLDSIALRLDNDFDLTGEIRRFFEDLRPAPLERAMRIAADP
                     RWAADVRSLVTRLRSRSETLTDRTTWRSAGLRLAARETGLAERSRNRRTRSLACAAAS
                     MGLATAVPAAPSGAVGATILVAAVAAAAMAGSLHDR"
     gene            1894..3408
                     /locus_tag="VGP50_17495"
     CDS             1894..3408
                     /locus_tag="VGP50_17495"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEV8029233.1"
                     /translation="MTVEAMEAAQGAAAALRVRLDIGGPLLVKLGHFLGTRPDLVDDA
                     YIDALVALPDRAPAPLPWETVRALLADAWSAAPEEALRSITVTPHAARILDQRHAATL
                     PDGTPVSVRIIRPDARALVAQIETQRHQMQEIVALCCAADRGAMAGVIGGFRTWLVGQ
                     MDLAAELRHRNRIAASGDEQGADVAVVSALCTPDILVTAEEPGRPFTPVGEASAGETQ
                     ALVRLAFRQIFRRGTGQDAPLASNITKESGRPAEWIGRGPYRPLTSIERQDVRALWSG
                     LHAHDEASVLRAVLRSLQTSEKPALERLELLILSAMRRDVAARPEHKATTRRDSHGVD
                     DVLLGILRAVRQTGLTMADGPLSAHRLLVATYRIVRLASAEIDPDELGRRCLAKLDIR
                     DALDTLDLDQMGKVAVDLLLLLRDAPERLNQIAADLAQGSLGITLALDESSSTARAWN
                     RRVRLIAIAIASLGPFFLLGEIGTAELWGIPAYVALGPLLAVLYGWMLLQWRKL"
     gene            3393..4790
                     /locus_tag="VGP50_17500"
     CDS             3393..4790
                     /locus_tag="VGP50_17500"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV8029234.1"
                     /translation="MAEALTSGRRTVVVGGGVAGLLAAIAVARHSAEVIVVERDIPPD
                     DALPRKSVPQGHHVHTLLLGGRTAIETLCPGVHREILAAGAQPIDFCADVAFFHHGVW
                     KSRCRSEFLGTLQTRPLLERILWGRVQSLGNVAIWTGVAQRLVATADGRRVVGVEVVD
                     PRGMARVLLGDIVIDASGRGSRLPAWLAELGLAPPVEETIRFDLRYASCLCRLRDDAR
                     RDWRALLVYPKAPFGRRAGYIFPVEGGRHLVTLAGYFGEAPRPDFGDFLTFARTLPRR
                     ELYDAIADAEPLSKIRLFHLAAQRRRRYDLLRHMPEGLIALGDAVCVLDPLFGQGMTV
                     AARQALALGDFFAHQSSRVGRGAWSALERQIVRQTNLPWLLTSSEAFRYADAEGRRSP
                     LFRPLQWYAAKVFELSASDPRVYRAFMGLMHLVEPLSSVFRPRLALAVLRQALRSSRR
                     RLALPGAIPIARPAE"
     gene            complement(5323..6237)
                     /locus_tag="VGP50_17505"
                     /pseudo
     CDS             complement(5323..6237)
                     /locus_tag="VGP50_17505"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
     gene            complement(6297..6689)
                     /locus_tag="VGP50_17510"
     CDS             complement(6297..6689)
                     /locus_tag="VGP50_17510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029235.1"
                     /translation="MGDVLIAPPQGGPAMTSNTGGTKAPEHGDDDGEEKSSRERSNDF
                     VNNGVDAAVELFGGVADAFGSAVRKAGDEVDKDNVGRFGLKNGLLRGMVEGTAHFLDK
                     LPDVIRNTYDVLTSSDDEKKPAKARKPR"
     gene            complement(6878..8290)
                     /locus_tag="VGP50_17515"
     CDS             complement(6878..8290)
                     /locus_tag="VGP50_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV8029236.1"
                     /translation="MTDPIEQIKTLVGNKGFIADAQGMAPFLEEERGLFHGAARLVVR
                     PASTAETAAVVRLCAASRIPMVPQGGNTGLSGGGVPAEDGRAVVLSLGRMNRVRALDP
                     MDHTITVEAGSILADVQRAAAAADRLFPLSLGAEGSCQIGGNLATNAGGIAVLHYGTM
                     RELTLGLEVVLPDGQIWDGLRALRKDNTGYDLKQLFIGSEGTLGIITAATLKLFPRPR
                     SVETAFLGLGRVEDAMALFAEARASSGDQLTAFELIPRLGLDMALAHVRGVRDPLANA
                     CPWYVLLEVSSSRSESGLRASLEAFLAETMATGLLRDGVVAASAAQGRDLWRIREGMV
                     EAQKHEGGSIKHDVSVPVSRVADFILRAIAAVEARLPGIRPLAFGHVGDGNIHFNLSQ
                     PPRADTAVYLARRPEFNHLVHDLVQSLGGSISAEHGIGRLRREELKRYKSPLELALMR
                     RLKQAFDPDDLMNPGKILPP"
     gene            complement(8287..9369)
                     /locus_tag="VGP50_17520"
     CDS             complement(8287..9369)
                     /locus_tag="VGP50_17520"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEV8029237.1"
                     /translation="MAAPPPEKGLARLRGVATQDLLRSPFHDYISPLRSGVREAAVTE
                     CLTPADLDRAAALLRAGELVAFPTETVYGLGADATSDRAVAAIFAAKDRPRFNPLIIH
                     VIDGAAAARLVLFDDRARRLAEHFWPGPLTLVLPRRPDDPVSLLASAGLDSLAVRVPA
                     HPLPRALIRAAGVPIAAPSANRSGRLSPTTAAHVLDELGGRIAAVLDGGACRVGLEST
                     VLDLTTAPPRLLRLGAVAPEQIEAVIGAIARVGDDDTAPRSPGMLASHYAPSLPLRLE
                     ARTVERREALLAFGKRAPAGAKEVRWLSRSSDLKEAAANLFAGLRALDRPEFSGIAVM
                     PIPEEGLGLAINDRLRRAAAPRKGAP"
     gene            9368..>9541
                     /locus_tag="VGP50_17525"
     CDS             9368..>9541
                     /locus_tag="VGP50_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV8029238.1"
                     /translation="MAGQIITIAQQKGGAGKTTLAAHLALAWAGKRSVSVIDIDPQGS
                     LAAWFRLREQQAGT"
BASE COUNT         1402 a         3219 c         3312 g         1608 t
ORIGIN      
        1 cggcgatctc ggcaatgccg gcgatctcgt tgagcgcgaa gcgcatatcg gcgagcgggg
       61 cggcgtagtc ggtcacggct gggttccctg ttgggggccc gcgccggcgc gggccgaaag
      121 acgcggatgc cataacgcgc cgggagcggc cgaagcaagc atcgttgcgg ccgacgcatg
      181 ccggaagatg cagcccgcaa gcgctggcgt cgggccgcga cgtcgcctta tcatgccgct
      241 cttcgccagg ccggaggctc gaggcgccga tgcgcaaatc ttggcgggac ggcgggtatc
      301 gttggcagcg cctcgcgctg ctggcggcga ccgccttcag gcatggcgcg cctctcgcct
      361 tcgatctgct gcgcggccgt ccggtgcgcc aggaggatgt cgccaggcga cttcgcgcgg
      421 cgctcgaggc gctcggcctc acctacctga agctcggtca attccttgca acgcgccatg
      481 acctggtgcc ggcggcggtg gtacgcgagc tgaccgctct gttcgaggac gtgccggctt
      541 tgcccgcggc gacggtggcc ggcatcgtcg cgcgcgagct cggcgcggcg cgcgatgggc
      601 tgttcgcttc cttcgacccg gttccggtcg ccgccgcaac ggtggcgcag gtgcatcgcg
      661 ccgagctgac ggacggaagc agcgtggccg tcaaggtgca gcggcccggc atcgaggccc
      721 tgttcgaagc ggacatctcc attctccgca gcctcgcgcg cctcggcgat cggctcgggc
      781 ttctcggatc gctttccgcg gtcgcgatcg ccgaccagtt cgccgcctat acgcgccgcg
      841 agttcgactt cctgctggaa gcgtcgaccg cggagcgctt gcgccgggag gcgagaccgg
      901 aatgcatcgt gccccgcatc gtccccgagc tcacgacgcg gcgcgtgctg acgatgcagt
      961 tcatggaggg cgtgagcctg gcgcgcatct gcaccttggt ggagagcggc gatgaggcga
     1021 cgttgcgcgc cgtgctgccg tcattcgacc gccggcgcgt tctcgagaac ctgacgctcg
     1081 tctcgctgca ccagttcttc gtcaccggct ttttccacgg cgacccgcac cccggcaata
     1141 tcctcgtatg ccccgacaat cgcgtcgtcc tgctcgattt cgggattttc ggcgagctcg
     1201 gcgccgccgg ccgcgacctg ttggcaagct acgcggagca cttggccatg ggaaatgtcg
     1261 ccgagagctt tcgtcatctg tcgcagatct actttcccgg tccgcgcagc gaccggcgcg
     1321 cgttccgcca ggatgcaatc gccgcgctca ggcgctggta cgtcgcctcg cgcgacccgt
     1381 cgacgccgat gagagagcgt catctcggcg gctccttcga tgccatggtc ggcgtcgtcc
     1441 gccgtcaccg atattggacg acgatggatt atctgctctt ctggcgtgcg atcatcgtgc
     1501 tcgattcgat tgcgctcagg ctcgacaacg acttcgatct caccggcgag atccggcgtt
     1561 tctttgagga tctgaggccg gcgccgctgg agcgtgccat gaggattgcc gccgatccgc
     1621 ggtgggccgc ggacgttcgc tccttggtca cccgcctgcg ttcccgatcc gagacactga
     1681 cggacagaac gacatggcgc tcggccggct tgcgtctggc cgcgcgagag acaggtcttg
     1741 ccgagcgttc ccgaaaccgc cggacgcgct ctctcgcctg cgccgcagcg agcatgggct
     1801 tggcgacggc cgtgcccgcg gcgccctcgg gcgccgtcgg cgcgacgatc ctggtcgcgg
     1861 ccgtcgccgc cgccgccatg gccggatcgc tccatgaccg ttgaggccat ggaagcggca
     1921 caaggcgccg ccgcggccct tcgcgtccgc ctcgacatcg gcgggccgct cctcgtgaag
     1981 ctcgggcatt ttctcgggac gcgaccggat ctggtcgatg atgcctatat cgacgcgctg
     2041 gtcgcgctgc ccgaccgcgc gccggcgccg ctgccgtggg agaccgttcg cgctctcctg
     2101 gccgacgcgt ggagcgccgc gcccgaggag gcgctccgca gcatcacggt cacgccccat
     2161 gcggcgcgca tcctcgatca acggcacgcg gcgacacttc cggacgggac gccggtcagc
     2221 gtgcgaatca tccgtcccga cgcgcgggcg ctcgtagcgc agatcgagac gcaacgtcat
     2281 cagatgcagg agatcgtcgc gctttgctgc gcggcggacc gcggcgcgat ggccggcgtg
     2341 atcggcggct ttcggacttg gctcgtcggc cagatggacc ttgcagcgga gttgcgtcat
     2401 cgcaatcgca tcgcggcgag cggcgacgag cagggcgccg acgtcgccgt ggtgtccgcc
     2461 ctgtgcacgc cggacatatt ggtcaccgcg gaagagccgg gacgaccgtt cacccccgtc
     2521 ggcgaggcga gcgcagggga gacccaggcg ctcgtccgtc tcgccttccg gcagattttc
     2581 cggcgtggca ccggccagga cgcgccgctc gcatccaata tcaccaagga aagcggtcgg
     2641 ccggcagagt ggatcgggcg cggcccgtat cgcccgctga cgtcgatcga gcggcaggac
     2701 gtaagggcgc tctggtcggg tctccacgcc catgatgagg cgagcgtgct gcgtgccgtc
     2761 ctgcgcagcc ttcagacgag cgagaaaccg gccttggagc ggctcgagct cttgatcctc
     2821 tccgcgatgc gccgggacgt cgcggcccgg ccggagcaca aggccaccac ccgccgcgat
     2881 tctcacggcg tcgacgacgt attgctcggc atcctgcggg cggtgcggca gaccgggctc
     2941 accatggccg acgggccgct ttcggcccat cgtctgctcg tcgccacgta ccggatcgtt
     3001 cgtttggcga gtgcggagat cgaccccgac gagctcggcc gccgctgcct ggcgaagctc
     3061 gacatcaggg acgcgctgga caccctcgat ctcgaccaga tgggcaaggt cgccgtcgat
     3121 ctgcttcttc tgctgcgcga cgcgcccgag cggctgaacc agatcgccgc cgatcttgcc
     3181 cagggcagtc tcgggatcac gctggcgctc gatgaatctt cgagcaccgc ccgcgcgtgg
     3241 aatcgccgcg ttcgcctgat cgccatagcg atcgcgtctc ttggaccgtt ctttctcctc
     3301 ggcgagatcg gcacggccga gctctgggga attcccgcct atgtcgcctt ggggccgctg
     3361 cttgccgtcc tctatggctg gatgctgctg caatggcgga agctctaacg agcggccgcc
     3421 gcaccgtggt ggtcggcggc ggcgtcgccg gcctcctcgc cgccatcgcc gttgcgcggc
     3481 attccgccga agtgatcgtc gtggagcgcg acatcccgcc cgatgacgct ctgccgcgca
     3541 agagcgtgcc gcaagggcac cacgtccaca cgctcctgct cggcggacgg acggcgatcg
     3601 agacgctgtg tccgggagtg caccgggaaa tcctggccgc gggcgcgcag ccgatcgatt
     3661 tctgcgccga cgtggcgttc tttcatcatg gcgtctggaa atcacgctgc cgcagcgaat
     3721 tcctggggac tctgcagacg cgcccgctgc tcgagcgcat cttgtggggc cgcgttcaat
     3781 cgcttgggaa cgtcgccatc tggacgggcg tggcccagcg cctggtcgcc accgcggacg
     3841 gccgtcgcgt cgtcggtgtc gaggtggtcg accctcgggg catggctcgg gtccttctcg
     3901 gagatatcgt gatcgacgcg agcggccgcg gctcgcgcct gccggcctgg ctcgccgaac
     3961 tcggacttgc ccctccggtc gaggagacca tccgcttcga cctgcgctac gcaagctgcc
     4021 tctgccgcct tcgcgacgac gcgcgccgcg actggcgtgc gctcctcgtt tatccgaagg
     4081 caccgttcgg ccgccgtgcc ggctatatct ttcccgtcga gggcgggcgc cacctcgtga
     4141 cattggccgg ctatttcggc gaagcgccgc gaccggactt cggtgatttc ctgaccttcg
     4201 cccggacgtt gccgcgccgc gagctctacg acgcgatcgc cgacgccgag ccgctctcga
     4261 agatccgcct attccatctc gcggcgcagc gccggcggcg atacgacctt ctgcgtcaca
     4321 tgcccgaggg gctgatcgcg ctcggcgacg ccgtctgcgt gctcgacccg ctgttcggcc
     4381 agggcatgac ggtggccgcc cggcaggcgc tggcgctcgg cgatttcttc gcgcatcaga
     4441 gcagcagagt cggccgcggc gcgtggtctg cgctggagcg ccagatcgtg cgacagacga
     4501 atctcccgtg gttgctcacc tcgagcgagg cgttccgata tgccgatgcg gaaggacggc
     4561 ggtcgccgct gtttcgtccg ctgcaatggt acgccgcaaa ggtgttcgag ctgtccgcat
     4621 ccgatccgcg cgtttaccgc gctttcatgg ggctcatgca cttggtggag cctctatcgt
     4681 cggtattccg gccgcggctg gcgctggcgg tcttgcgaca ggcgctgcgg tcgagccgcc
     4741 ggcgcctcgc cctgccgggc gcaattccca tcgcgcgtcc ggccgagtga gatcggatct
     4801 tggccgctcg gccggcgggg cgacggcgac cggatgcagg cgcggggtca gcgccatttc
     4861 cggccgatat tcctcatagg cgtgccagcc ggcacggcac cattcccgcg ccgccgcgat
     4921 tggcgcgcgc aattcggcct tgagcgcgcg cagttgcgga tcgaagccgc tggcgaacag
     4981 gcgcacgccg cctgtcgaca cgcgccgcgt gccgttgaag gcgatgatct tggccagcgt
     5041 ccgcagggcg cgacgcgacg tcgagagctg acggtccgcc cagaactgca gctccttcat
     5101 ctgctccggc gagatcgcgg gatgattcca caccaggttc cgggaattgt acttgctcag
     5161 gttatggtcg aagatcccgt gcgtctgctc gatcatcttg cgctgggccg ttcccgggta
     5221 aggggtcagg atctgaacct gaacgagatc gacgtcaagg cgtgcgagtt cgaggatgtc
     5281 gcgctgaatc gacgcgacgg tgtcctcctc gaatccgagg atgtagaagg cctggacgaa
     5341 catgttgtgg ctgctcatgc ggcgcaggag atcgagcgtg cattgctgct cgacgcgctt
     5401 cccggctccc ccgagcgagt ccgcgttcag gctctcgatg ccgagatgcg cgcccatcag
     5461 gccatgcgcc tgccaatggt cgaaattctt cgtcagcgtg tcgacgcgcg tgagcgcgat
     5521 ccaccgcatc ccatagcggt gcagcagctc gaccacgcgg ttcgcgtggc ttctgaacgt
     5581 cccgaagttc tcgtcgagaa tgttgatcgc gcgcacgccg cgcgcgtgat agacgccgat
     5641 gatccgcttg atctcgtcga gcgggatcgg ctgcggcttg ccgtagaaat ccggcgtttg
     5701 acagaaattg caggtccacg ggcatccgcg cgacgtgtac agcgtgcccg acaggatgac
     5761 gggcgtcgcg aggttggtgc cgaaggcgcc gtagagctcg ggatggacga gcggccccgg
     5821 ccggttgtcg agcgccagcg cttgcgccgc ggcgacttcg ctccatcctc tgaacacgcg
     5881 atcgaaggcc gacgtgattt ccgggctgtc ggcgccgtag ttgcccgccc atacctcctt
     5941 cacgccgcgt gcgcgcgcct gcctcgccat gtcgagggca atctcggttt cattgatgtg
     6001 gaaggagatg ccgacgacat cgggtctggt ctcgagcgcc tcctcgaagg ctcggcgcga
     6061 gggatactcg agaatgtcga ccggcagatt ggccttgaga aatccgaggc cgggatgatt
     6121 caagaagcag agaaacagcc gcagcgcgcc cggccgcttg ttccaactat agacgtcggg
     6181 ggagcagccg ccgatcggct gtatcggcga agtcagaagc acccttcgag cagtcatgcg
     6241 caacctccat cgcgcgagat gaacgcgtgc gagcccttgc ggaattttcg actagatcac
     6301 cggggttttc gggccttcgc aggcttcttt tcgtcgtcgg aggatgtcag aacgtcgtag
     6361 gtgttccgaa tcacgtccgg cagcttgtcg agaaaatgcg ccgtgccttc gaccatgccg
     6421 cgcagcaagc cgttcttcag gccgaaccgg ccgacattgt ctttgtcgac ctcgtcgccg
     6481 gccttgcgta ccgcgctgcc gaacgcgtcg gcgacgccgc cgaaaagctc caccgcggca
     6541 tcgacgccgt tattgacgaa gtcgttcgag cgctcgcgtg acgacttctc ctcgccgtca
     6601 tcgtcgccgt gctcgggcgc ctttgtaccg ccggtgttgc tggtcatcgc cggccctcct
     6661 tgtggcggcg cgatgagcac gtcgcccatc tgaacgactc tcacgacggc gcgtcgcgct
     6721 cctaaggata gaactacgtg atactattgc cgctggcaat ggcgaaatgc aactaaaatg
     6781 ggtttttaat gactcatgct ctcgcatgag gagcggtgct gtcgcgcgct tgggagtttc
     6841 gttgcaagca gtgacgccgg acgggtgaac gccaccgtca cggcggcaag atcttgcccg
     6901 gattcatcag gtcgtcggga tcgaatgcct gtttcagccg gcgcatcagc gcgagctcga
     6961 gcggcgactt gtagcgcttg agctcttcgc ggcgcaggcg gccgatgcca tgctcggcgc
     7021 tgatcgagcc gccgaggctc tgcacgagat catggacgag gtggttgaac tcaggccggc
     7081 gcgcgagata gacggcggtg tcggcgcggg gcggctggct cagattgaag tggatgttgc
     7141 cgtcgccgac atggccgaag gcgagcggac ggatgcccgg cagcctcgcc tcgacggcgg
     7201 cgatcgcgcg caggatgaaa tcggcgacgc ggctcaccgg caccgacacg tcatgcttga
     7261 tgctgccgcc ttcgtgcttc tgcgcttcca ccatgccttc gcggatgcgc cagagatcgc
     7321 gcccctgcgc cgcgctcgcg gcgaccacac cgtcgcgcag caggccggtg gccatggtct
     7381 ccgccaggaa cgcctcgagc gaggcgcgca gaccgctctc gctccggctc gacgagacct
     7441 cgagcagcac ataccaggga caggcattgg cgagcggatc gcgcacaccc ctgacatggg
     7501 cgagcgccat gtcgaggccg aggcgcggga tgagctcgaa ggcggtgagc tgatcgccgc
     7561 tcgaggcgcg cgcttcggcg aacagcgcca tggcatcctc gacgcggccc aatccgagga
     7621 acgccgtctc gaccgagcgc ggccgcggaa agagcttgag cgtcgccgcg gtgatgatgc
     7681 cgagcgtgcc ctcgctgccg atgaagagct gcttcaggtc ataccctgtg ttgtccttgc
     7741 gcagcgcgcg caggccgtcc cagatctggc cgtccggcag caccacttcg agcccgagcg
     7801 tcagctcgcg catcgtgccg taatgcagca cggcgatgcc tccggcattg gtggcgagat
     7861 tgccgccgat ctggcacgag ccttcggcgc cgaggctcag gggaaagagt cgatcggccg
     7921 cggcggcagc gcgctggacg tcggcgagga tagagcccgc ttcgacggtg atggtgtggt
     7981 ccatcggatc gagcgcgcgg acgcggttca tgcggccgag actcagcacc accgcgcgtc
     8041 cgtcctccgc gggcacaccg ccgccggaga ggccggtgtt gccgccttgc ggcaccatgg
     8101 gaatgcgcga agccgcgcag agccgcacca ctgccgccgt ttccgcggtc gaagccggac
     8161 gcacgacgag gcgcgccgcg ccatgaaaga ggccgcgctc ttcctcgaga aaaggcgcca
     8221 tgccctgggc atcggcgatg aagcccttgt tgccgacgag agttttgatc tgctcgatcg
     8281 ggtcggtcat ggcgcgcctt tgcgcggcgc ggcagcgcgg cgcagccggt cgttgatggc
     8341 gaggcccagc ccttcctcgg ggatcggcat gacggcgatg ccgctgaatt ccggccggtc
     8401 gagcgcgcgg agcccggcga agagattggc cgccgcttct ttgagatcgc tggagcggct
     8461 cagccagcgc acttccttgg cgccggcggg cgcgcgcttg ccgaaggcga gcagcgcttc
     8521 gcggcgttcc accgtccttg cctcgagccg caacggcagc gacggcgcgt aatggctcgc
     8581 cagcatgccg ggcgagcgcg gcgcggtgtc gtcatcgcca acgcgcgcga tagcgccgat
     8641 aacggcctcg atctgctccg gcgcgaccgc gcccagccgc agcaggcgcg gcggcgcggt
     8701 cgtcaggtcg agcacggtcg attcgagccc gacccggcag gcgccgccgt cgagcacggc
     8761 ggcgatgcgg ccgccaagct cgtcgaggac atgggcggcc gtggtcgggc tgaggcggcc
     8821 ggagcggttg gcgctgggcg cggcgatcgg cacgccggca gcgcggatca gcgcgcgcgg
     8881 cagcggatgc gcgggaacgc gcacggcgag gctgtcgagg ccggcgctgg ccagcagcga
     8941 caccgggtcg tccggccggc gcggcaggac gagcgtcagc ggcccgggcc agaagtgctc
     9001 ggcgagtcgg cgcgcccgat cgtcgaagag gacgagtcgc gccgcggcgg cgccgtcgat
     9061 gacgtggatg atgagcggat tgaagcgcgg ccggtctttg gccgcgaaga tcgcggcgac
     9121 ggcgcggtcg ctggtcgcgt cggcgccgag gccgtagacg gtctctgtcg ggaaggccac
     9181 cagctcaccc gcccgcagca gcgcggccgc gcggtcgaga tcggcggggg tgagacattc
     9241 ggtcacagca gcctcgcgca cgccgctcct gaggggcgag atatagtcgt ggaaaggcga
     9301 gcgaagcaaa tcctgcgtcg cgacgccgcg gaggcgcgcc aacccttttt ccggaggagg
     9361 tgccgccatg gccggccaga tcattaccat cgcccagcag aagggtggtg ccggcaagac
     9421 gacgctcgcc gcccatctgg cgctcgcctg ggctggcaag cggagtgtct ccgtcatcga
     9481 catcgacccg cagggcagcc tcgccgcgtg gttccgcttg cgggagcagc aagcgggcac
     9541 g
//
LOCUS       DASYNS010000577         9595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1553221, whole genome shotgun sequence.
ACCESSION   DASYNS010000577 DASYNS010000000
VERSION     DASYNS010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553221"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..621
                     /locus_tag="VGP50_17530"
     CDS             244..621
                     /locus_tag="VGP50_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029239.1"
                     /translation="MLNRIELRPIKTEADYDAALAAIDRLTAGDREPEEGTPESDLIE
                     ILITLVQAYESLHYPMAAPDPIEAIKFRMEQRADPARPRAGDRRRRTRLGSTEPAPCA
                     DPPHDPPPQRNVRHPRGIAHREV"
     gene            complement(677..751)
                     /locus_tag="VGP50_17535"
     tRNA            complement(677..751)
                     /locus_tag="VGP50_17535"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(717..719),aa:Thr,seq:ggt)
     gene            935..2167
                     /locus_tag="VGP50_17540"
     CDS             935..2167
                     /locus_tag="VGP50_17540"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV8029240.1"
                     /translation="MFDRRKLLLGMGGTALSAAGLWPERAAGAGDPVLATGLPQGVYD
                     TAALEALPGKKPLIRLSYRPPNYETPAGYFASAFTPNDAFFIRYHLADIPEAPDAAAW
                     KIRVGGEGAASPVELGLADLASGFEQVEIAAVCQCSGNRRGLFDPHVPGVQWGPGAMG
                     NARWTGVRLKDVLAKAGLRKEAVEIVVNGADGPVLDKTPPFVKSIPLWKALDENTLIA
                     WAMNGEPLPRLNGFPVRLVVPGWTATYWMKHLSSVEIATKPFDGFWMKTAYRIPTGKF
                     PVMQHFLTQMTETSEPITEIVVNSLVAAPAPGHRAKAGRPLDIKGVAWDGGYGIRRVE
                     LSVDGGTNWREAALGADDGRFAFRPWSFRFTPARPGAVTVMARASNSVGQTQAEVLIF
                     NPAGYHNNVVRPLTVTVV"
     gene            2179..2472
                     /locus_tag="VGP50_17545"
     CDS             2179..2472
                     /locus_tag="VGP50_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEV8029241.1"
                     /translation="MRYGAVIAVALLGIGTVSLADGEERPVALAPGPGLDAMANCAAC
                     HSLDYIRINAPFMTRKAWEAEVAKMITVFGAPIDPADAKTIADYLARNYGKPD"
     gene            2738..5203
                     /locus_tag="VGP50_17550"
     CDS             2738..5203
                     /locus_tag="VGP50_17550"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEV8029242.1"
                     /translation="MSVEPLSLARYVRSLTGRPPHAARPEDWLAAIAAAVREHVMSHW
                     AGTGPHPRAAGGKRVYYLSMEFLPGRVLLHALMSLGLHDACRRTLAEHGVDLDELAEL
                     EVEPALGNGGLGRLAACLLDSMANLGLSACGYGIRYGCGLFSQQIQDGWQVERPDLWL
                     DAGSSWELPRRDLVYRVKFGGRVMAPHGAAHERRDWVDTDEVLATAYDLPVPGYRSQR
                     VGNLRLWAPRAAHEFDLKSFNDGDHGGAFSRKNAIESLSQVLYPGDATLSGRELRFKQ
                     EFFFVSASIQDILRRFRRRHESFDRLPDEIAIQINDTHPSLAIAELMRLLIDEHGCDF
                     ERAWHITRQCFAYTNHTLMPEALEVWPVRFFETLLPRHLQIVYDINDWHLRRAASRRP
                     DDGALLRRISLVNEDGERSIRMAHLAVVGSHKVNGVSKMHTALMQQTIFADFHALDPA
                     KIVNVTNGITFRRWLHEANPDLTRLISSRIGKRWLTHADDLAQLAPHAEDPAFRAAFR
                     TVKRDNKKRLAELIARECNIAAEVDSLFDVQVKRIHEYKRQLLNLLHVVALYRRLREG
                     RLAGTLARTVIFAGKAAPGYAVAKLTVKLIHDVAAIVNGDPAMAGRLKVVFIADYGVG
                     KAQKIIPAADLSEQISTAGYEASGTGNMKLALNGALTIGTLDGANIEIREAVGEENFF
                     LFGADAADVARLRAGRYDPSAFYHGDPELKGALDLVASGHFSPSQPDLFKPIVDALIQ
                     HDPYLVLADFAAYRRCQERVEALYGDVEEWSRRAILNVAGVGEFSSDRAVQQYMQRVW
                     NMGAPSHHAAPETGVPRALETIG"
     gene            complement(5220..7409)
                     /gene="malQ"
                     /locus_tag="VGP50_17555"
     CDS             complement(5220..7409)
                     /gene="malQ"
                     /locus_tag="VGP50_17555"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV8029243.1"
                     /translation="MSALDALAARAGIEPYYHDIWGGRHALSPETKRIFLDAMGLSAA
                     SEEEAAQSLAAFATTPWRRALEPVTVLRAGSPEWAADIVCDAARAGAVTWRIVTEDGT
                     RQEGTVSLADLPVAETQRIDGRPLERRRLALPASLPLGYHRIEIAGDALPGGSDIAML
                     IAAPPHAYLPETLQHPPGIWGVNLQLYGLQGRESWGIGDFGDLAGFAAAAGSAGTGAL
                     GLNPLHALFPGNPAHASPYSPSSRCFLNPLYIDIAAVPDLAECPEARDQLESAAFRAS
                     LAALRAAPLVDYVAVAAAKAPMLERLYEAFRANHIERGTARAEAFRRFQRESGIALER
                     HTTFEALAEQFRAGKAGHLPWRQWPAPYRDPNAPETVAFARDHAERVTFHEYLQWEAD
                     RQLGAAASACRQGGMSVGLYRDLALGIDGDGAEAWAEQALVAEGISTGAPPDPFNLKG
                     QSWGLPPFAPHALRDAAYRPFIAGLRANMRHAGALRIDHVMGFARLFWIPGGALPAEG
                     GYVRYPLDEMLAVTALESQRARCAVIGEDLGTVPEGLRERLSAERVLSMRLLYFERDD
                     DGRFRGAETYPERAHVAIGTHDLPTFPAYWREDDIALRAALNLLPSPDSEAMLRQDRA
                     RMRAALLSHLRRLGLLPPAENPAGDRSIDMATLVDAAYRFLATAPSPLLMVQPEDVLG
                     VLEQINLPGTVDEHPNWRRKLPMPWQDIVANRRWRDFAAMLSRLRPR"
     gene            complement(7406..9559)
                     /gene="glgX"
                     /locus_tag="VGP50_17560"
     CDS             complement(7406..9559)
                     /gene="glgX"
                     /locus_tag="VGP50_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011389296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEV8029244.1"
                     /translation="MTKGQRTRVWPGEPFPLGATWNGKGVNFALFSENAERVELCLFD
                     PSGESETDRIDLREYTNDVWHGYLPDVVPGQLYGYRVFGPYVPERGHRFNHHKLLIDP
                     YARMLEGQFAWNDAVFGYDTASPDADLSFDTRDSAAFVPKCRVVDPAFTWGDDRPPQR
                     PWQDTVIYELHVRGFTMRRPDVPEALRGTFAGLTTPAVIGYLRDLGVTAVELLPVQAF
                     FDEVPLVKRGLQNFWGYNPIAFFVPQARYLAAQTLHEIRTAVHRLHDAGLEVILDLVF
                     NHSAEGGALGPTFSFRGIDNASYYRLDRDKPRAYRDFTGCGNSLNLHHPRVMQLVMDS
                     LRYWVTEMHVDGFRLDLATTLARGEDGAFDRYSGFLDAVLQDPVLAQVKLIAEPWDLG
                     DQGYRLGQFPPGWSEWNDRYRDVVRRFWRGEDGLVAELATRVTASRDIFDQWGRRPWA
                     SVNYVAVHDGFTLEDLVSYREKHNENNGEGNRDGAGENYSANYGAEGPTADPLIRQLR
                     RRQKRNMLATLLLSVGVPMLLAGDEFGRSQNGNNNAYCQDNEISWLDWSLLEGEDGAT
                     LRDFVRRLLRLRNEHMVFRRRHFFHGHLIPGTAVKDIVWLTPDGGERRDDDWKASGAR
                     FLSYVVSGQAGTIHHSMSGEPQSDDSFLVILNADSEAVSCLLPETSPPGPWIKLVDTA
                     KESGSGDEASFAAGARYTVEPQSFLLFCRPSSSAP"
BASE COUNT         1559 a         3192 c         3263 g         1581 t
ORIGIN      
        1 ttcgggcagc accacctcgc ccggcgccac tggctcgccc ggcgggacca cgagcggctc
       61 gggcatctcg cgctgaggtt cgacccttga cctgatctcg gagccccgct tcggcggggc
      121 tctttgattc atgctgccga aatgccgtgc cgtgctatcg gcaccatgcg atctatgtgc
      181 gcttcatcgg cacccatgcc gagtatgacc ggatcgatgc caacgaggtt taggaggctc
      241 tgcatgctca acaggattga actgcgcccg atcaagaccg aggccgatta cgatgcggcg
      301 cttgccgcca tcgaccggct caccgcgggc gatcgcgagc cggaggaggg gacgcccgag
      361 agcgacctga tcgagatcct gatcaccttg gtgcaggcct atgagagctt gcattatccg
      421 atggcggcgc ccgatccgat cgaggccatc aagttccgta tggagcagcg ggctgacccg
      481 gcgcgacctc gagccggcga tcggcggcga cggacgcgtc tcggaagtac tgaaccggcg
      541 ccgtgcgctg accctcccca tgatccgccg cctcaacgca atgttcggca tccccgcgga
      601 atcgctcatc gggaagtatg acgcggcgga gtgaggattg agcctcatga tctcgcgcct
      661 tccagcgaag caattatggt gctgccgcac aggattgaac tgtggacctc tcccttacca
      721 agggagtgct ctaccactga gctacggcag cagccttgtg cgtcgagggc gccggggcgc
      781 ctgagccagc gtggccgcag gagatatagg atcgggcgag gcttggcaag gcgtcacccc
      841 ctcagcgtaa agcgccaaat gcgggctggt ggatcggcag gtcttgccgc atcctgatga
      901 tcgagcccgg ccgcgcctgc gcgggaggag tggattgttc gatcgtcgca agctgctgct
      961 cggcatgggc gggaccgcgc tctcggccgc ggggctgtgg ccggagcgtg ccgctggcgc
     1021 gggcgatccg gtgctggcga cggggctgcc ccagggcgtc tacgacaccg cagcgctcga
     1081 agcgctgccg ggcaagaagc cgctcatccg gctgagctat cgcccgccga actacgagac
     1141 gccggccggc tatttcgctt cggccttcac gcccaacgac gcgttcttca tccgctatca
     1201 cctcgccgat atccccgagg ctcccgatgc cgcggcgtgg aagatcagag tcggcggcga
     1261 gggcgcggcg tcgcccgtcg agctcggtct ggccgacctc gcaagcggct tcgagcaggt
     1321 cgagatcgcc gccgtgtgcc aatgctcggg caatcgccgc ggcctctttg atccgcatgt
     1381 gccgggcgtc cagtgggggc cgggcgccat gggcaatgcg cgctggacag gcgtgcggct
     1441 caaggacgtt ctggcgaagg cgggcttacg gaaagaggcg gtcgagatcg tcgtgaatgg
     1501 cgcggatgga ccggtgctcg acaagacgcc gcccttcgtc aagagcatcc cgctgtggaa
     1561 ggcgctcgac gagaacacgt tgatcgcctg ggcgatgaac ggggagccct tgccgcgtct
     1621 caacggtttc ccggtgcgac tcgtggtgcc cggatggacc gccacctatt ggatgaagca
     1681 tctgagcagt gtcgagatcg cgaccaagcc cttcgacgga ttctggatga agaccgcgta
     1741 tcgcattccg acgggcaagt ttccggtgat gcagcatttc ctcacgcaga tgaccgagac
     1801 gagcgagccc atcaccgaga tcgtcgtcaa ttctctcgtc gccgctcccg cgccggggca
     1861 ccgggcgaag gccgggcggc cgctcgacat caagggcgtc gcctgggacg gcggctacgg
     1921 catccgccgc gtcgaactgt cggtcgatgg cggcaccaac tggcgcgagg ccgcgctcgg
     1981 cgccgatgac gggcgcttcg ccttccggcc ctggagcttc cgcttcaccc cggcaagacc
     2041 gggcgccgtc accgtgatgg cacgggcgag caacagcgtc ggccagactc aggccgaggt
     2101 gctgatcttc aacccggcgg gctatcacaa caatgtggtc cgcccgctca ccgtcacggt
     2161 cgtctgaaga tgtcccggat gaggtacggc gccgtgatcg ccgtcgcgct cctcggcatc
     2221 ggaaccgtgt cgctcgccga tggcgaggaa aggccggtgg cgctcgcccc cggtccgggc
     2281 ctcgacgcca tggccaattg cgcggcctgt cacagcctcg actacatccg catcaatgcg
     2341 cctttcatga cgcgcaaggc gtgggaggcc gaggtcgcga agatgatcac cgtcttcggt
     2401 gcgccgatcg atcccgccga cgccaagacg atcgccgatt acctcgcgcg caactatggc
     2461 aagcccgact gaacgctccg cgcgcagacg ggcgggggat gcgggtcttg ggcatgcgat
     2521 ctctgtgtgg gaaatcacat cgatgatgct gcttccgccg taccattagt gtttcggtaa
     2581 ccatggcgcc cctagactct taacaccgag cggcgggacg gtcttggctc tcgcgtcccg
     2641 gtggtcgatt ggccagccca tactacggtt agagcaacac ggcgggtgat ggcccaaaat
     2701 gttgacaatc cagcccctga acgggatgat ttcatcgatg tccgtcgagc ccctctccct
     2761 cgcgcgctat gtccgctcat tgaccggtcg cccgccgcac gcggcaaggc cggaggattg
     2821 gctggccgcg atcgccgcgg cggtgcgcga gcatgtcatg tcccattggg ccgggacggg
     2881 accgcacccc cgcgcggccg gcggcaagcg cgtctactac ctgtcgatgg agttcctgcc
     2941 cggccgcgtt cttctccacg ccctcatgag tctgggcctc catgacgcgt gccggcggac
     3001 gctggcggag catggcgtcg atctcgacga gctcgccgag ctcgaggtcg agccggcgct
     3061 gggcaatggc ggactcggcc gcttggccgc gtgcctgctc gacagcatgg ccaatctcgg
     3121 gctgtcggcc tgcggttatg gcatccgtta cgggtgcggc ctgttctcgc agcaaatcca
     3181 ggacggctgg caggtcgagc gtcccgatct ctggctcgat gccggcagct cctgggagct
     3241 gccgcgccgc gacctcgttt atcgcgtgaa attcggcggc cgcgtgatgg cgcctcatgg
     3301 cgcagctcac gagcgccgcg actgggtcga caccgatgaa gtgctggcca cagcctatga
     3361 tctgcccgtc cccggatatc gctcgcagcg ggtgggcaat ctgcggctat gggcgccgcg
     3421 cgcggcccat gagttcgacc taaaatcctt caacgacggc gatcatggcg gcgccttctc
     3481 gcgcaagaac gccatcgaaa gcctgtcgca ggttctctat cccggcgatg cgacgctgtc
     3541 ggggcgggag ctccgcttca agcaggagtt cttcttcgtc agcgcctcga tccaggacat
     3601 tctccgccgc ttccgccgcc gccatgaaag cttcgaccgg ctgcccgacg agatcgccat
     3661 ccagatcaac gacacgcacc cgtcgcttgc catcgccgag ctcatgcgtc tcctcatcga
     3721 cgagcatggt tgcgacttcg agcgcgcctg gcacatcacg cgccaatgct tcgcctacac
     3781 caaccacacc ttgatgccgg aggcgctcga ggtctggccg gtccgcttct tcgagactct
     3841 gctgccgcgc catctgcaga tcgtctacga catcaacgac tggcacctgc gccgtgccgc
     3901 gagccggcgg ccggatgacg gcgccctgct gcgccgcatc tcgctggtga acgaggatgg
     3961 cgagcgctcg atccgcatgg cgcatctcgc cgtcgtcggc tcgcacaagg tcaacggcgt
     4021 ctccaagatg cacacggcgt tgatgcagca gacgatcttt gccgacttcc acgccctcga
     4081 ccccgccaag atcgtcaatg tcaccaacgg catcacgttc cgacgctggc tccatgaggc
     4141 gaatcccgac ctgacgcgcc tcatctcgtc gcgcatcggc aagcgctggc tgacgcatgc
     4201 cgacgatctc gcacagctgg cgccgcacgc cgaggatccc gccttccgcg cggcgttcag
     4261 gacggtgaag cgcgacaaca agaagcggct ggcggagctg atcgcgcgcg aatgcaacat
     4321 cgcggccgag gtcgattcgc tgttcgatgt ccaggtcaag cgtattcacg aatacaagcg
     4381 gcagctcctc aatctgctcc acgtcgtcgc tctctatcgc cgtctgcgcg agggccggct
     4441 tgccggcacc ctggcgcgca ccgtgatctt cgcggggaag gcggcgccgg gctatgccgt
     4501 cgcgaagctg acggtgaagc tgatccacga cgtcgccgcc atcgtgaatg gcgatcctgc
     4561 catggccggg cggctcaaag tcgtgttcat cgccgattac ggcgtcggca aggcgcagaa
     4621 gatcatcccg gccgccgacc tgtccgagca gatctcgacc gccggctacg aagcctccgg
     4681 caccggcaac atgaagctgg cgctcaacgg cgcgttgacc atcggcacgc tcgacggcgc
     4741 gaacatcgag atccgcgaag ccgtcggcga ggagaacttc ttcctcttcg gcgccgacgc
     4801 tgccgatgtg gcgcgtctgc gcgccggccg ctacgaccct tcggccttct atcacggcga
     4861 ccccgagctc aagggcgcgc tcgatctcgt cgcttccggg catttctcgc cgtcgcagcc
     4921 ggacctcttc aagccgatcg tcgacgcgct catccagcac gacccctatc tcgtgctggc
     4981 cgatttcgcc gcctaccggc gttgccagga gcgcgtcgag gcgctttatg gcgatgtcga
     5041 ggaatggtcg cggcgcgcca ttctcaacgt cgccggcgtc ggcgaattct cgagcgatcg
     5101 cgcggtgcag caatacatgc agcgcgtctg gaacatgggc gcgccgtcgc accacgccgc
     5161 gcccgagacc ggcgtccccc gcgccctcga gacgatcggg taaagcgcgc tccgaacgct
     5221 tatcgcgggc ggagccgcga cagcatggcg gcgaagtcgc gccagcggcg gttggcgacg
     5281 atgtcctgcc acggcatcgg cagttttcgg cgccaattcg gatgctcgtc caccgtgccg
     5341 ggcagattga tctgttccag cacgcccagc acatcctcgg gctgaaccat caagagcggg
     5401 ctcggcgcgg tggcgagaaa gcggtaggcg gcatcgacca gcgtggccat gtcgatgctc
     5461 cgatcgccgg ccgggttctc ggccggcggc agtaggccaa gtcggcgcaa atggctcaag
     5521 agcgccgcgc gcatccgcgc gcgatcctgg cgcagcatcg cctcgctgtc gggcgacggc
     5581 agcaggttga gcgcggcgcg aagcgcgatg tcgtcctcgc gccagtaggc gggaaaggtc
     5641 ggcagatcat gcgtgccgat ggcgacatgc gcgcgctcgg gataggtctc cgcgccgcgg
     5701 aagcggccgt cgtcgtcgcg ctcgaaatag agcaaccgca tcgacaggac gcgctcggcg
     5761 ctcaagcgct cgcgcagacc ctcgggaacc gtgcccagat cctcgccgat gacggcgcag
     5821 cgggcgcgct ggctttcgag cgccgtgacc gcgagcatct cgtcgagcgg atagcgcaca
     5881 tagccgcctt cggccgggag cgcgccgccc ggaatccaga agagccgggc gaagcccatg
     5941 acgtggtcga tgcgcagcgc gccggcatgg cgcatatttg cgcgcagtcc cgcgatgaag
     6001 ggccggtacg cggcatcgcg cagcgcatgc ggggcgaagg gcggcaggcc ccagctctgg
     6061 cctttcaagt tgaagggatc gggcggcgcg ccggtggaaa tcccttcggc gaccagcgcc
     6121 tgctcggccc aggcttcggc gccgtcgccg tcgatgccga gcgcgaggtc gcggtagagc
     6181 ccgaccgaca tgccgccttg gcggcaagcg ctcgcggcgg cgccgagctg gcggtccgcc
     6241 tcccattgca gatattcgtg gaaggtcacg cgctcggcat ggtcgcgcgc gaaggccacc
     6301 gtctccggcg cgttcggatc gcggtaaggc gccggccatt ggcgccacgg cagatgccct
     6361 gccttgcccg cgcggaactg ctcggccagc gcttcgaagg tggtgtggcg ctcgagcgct
     6421 atgccactct cgcgctgaaa tcggcgaaac gcttcggcgc gcgccgtccc gcgctcgatg
     6481 tggttcgcgc ggaaagcctc atagagccgc tcgagcattg gcgccttggc ggcggcgacc
     6541 gcgacatagt cgacgagcgg cgcggcgcgc agcgcggcga gcgacgcgcg gaatgcggca
     6601 ctctcgagct gatcgcgcgc ttccgggcat tcggcgagat cggggacggc cgcgatgtcg
     6661 atgtagagcg gattgagaaa gcaccggctc gacggagagt aggggctcgc atgcgcggga
     6721 ttgccgggaa agagcgcatg aagcgggttc aggcccaaag cgccggtgcc ggcgctgccc
     6781 gctgcggcgg caaatcccgc gaggtcgccg aaatcgccga tgccccagct ctcgcggccc
     6841 tgaagcccat aaagctggag attgacgccc cagatgccgg gagggtgttg cagcgtctcg
     6901 ggcaaatagg cgtgcggcgg cgccgcgatc agcatggcga tgtcggagcc gccgggcagc
     6961 gcatcgccgg cgatctcgat gcggtgatag ccaagcggca gcgacgcagg caaagcgagc
     7021 cgccgccgct cgagtggccg gccgtcgatc ctctgtgtct ccgccaccgg caaatccgcg
     7081 agcgagaccg tgccttcttg tcgtgtgccg tcctccgtca cgatgcgcca ggtcactgcg
     7141 ccggcgcgcg cggcatcgca gacgatgtcg gcggcccact ccggcgaccc cgcgcgcagc
     7201 accgtgaccg gctcgagcgc gcgccgccag ggcgtggtgg cgaacgctgc gaggctttgc
     7261 gccgcttctt cttcgctggc cgcggaaaga cccatggcgt cgaggaagat gcgcttggtc
     7321 tcgggcgaga gcgcatgacg cccgccccag atgtcgtggt aatagggctc gatgccggca
     7381 cgggcggcga gcgcgtcgag tgcgctcatg gcgcgctgct gctgggccgg caaaacagca
     7441 ggaaggattg cggctcgacc gtgtagcgcg cgccggcggc gaaggaggct tcgtcgccgc
     7501 ttccggactc cttcgcggta tcgaccagct tgatccaggg tccgggcggc gacgtctccg
     7561 ggagcaagca gctcacggct tcgctgtcgg cgttgaggat gacgagaaaa ctgtcgtcgg
     7621 attgcggctc gccgctcatc gagtgatgga tggtgccggc ctgcccgctc acgacatagc
     7681 tcaggaatct tgcgccggag gctttccaat cgtcgtcgcg gcgctccccg ccgtcgggcg
     7741 tcagccagac gatgtccttg acggcggtgc cggggatgag gtggccgtga aagaagtggc
     7801 ggcggcggaa caccatgtgc tcgttgcgca gccgcagcag ccggcgcacg aaatcgcgta
     7861 gcgtggctcc gtcctcgccc tcgagcagcg accagtcgag ccagctgatc tcgttgtcct
     7921 ggcaataggc gttgttgttg ccgttctggc tgcggccgaa ctcgtcgccg gcgagcagca
     7981 tgggaacgcc caccgagagc agcagcgtgg ccagcatgtt gcgcttctgc cggcgccgga
     8041 gctggcggat cagcgggtcg gccgtcggac cttcggcgcc gtaattggcg ctgtagttct
     8101 cgcccgcgcc gtcgcgattg ccttcgccgt tgttctcgtt gtgcttctcg cggtagctga
     8161 cgagatcctc gagcgtgaag ccgtcatgca cggcgacata gttgacgctg gcccaggggc
     8221 gccgtcccca ttggtcgaag atgtcgcgcg aggccgtgac acgcgtcgcg agctccgcca
     8281 ccagcccgtc ctcgccgcgc cagaagcggc gcacgacgtc gcgatagcgg tcgttccact
     8341 cggaccaccc cggcggaaat tggccgagcc ggtagccttg gtcgccgaga tcccagggct
     8401 cggcgatgag cttgacctgg gcgagcacgg ggtcctggag gacggcgtcg agaaagcccg
     8461 aataccggtc gaaggctccg tcctcgccgc gggccaatgt cgtcgcgaga tcgaggcgga
     8521 agccgtccac gtgcatctcg gtcacccagt aacgcaacga atccatgacg agctgcatga
     8581 cgcgcggatg atgcaggttg aggctgttgc cgcagccggt gaagtcgcgg tacgcgcggg
     8641 gcttgtcgcg atcgagccgg tagtagctgg cattgtcgat gccgcgaaag ctaaaggtgg
     8701 gcccgagcgc gccgccttcc gcgctgtggt tgaagacgag atcgaggatg acttcgaggc
     8761 cggcgtcgtg caggcgatgc accgccgtcc tgatctcgtg cagcgtttgc gccgcgaggt
     8821 agcgcgcctg cggcacgaaa aaggcgatcg ggttgtagcc ccagaagttc tgcaatccgc
     8881 gtttgacgag cggcacttcg tcgaagaaag cctgcaccgg cagaagctcg acggcggtca
     8941 cgccgaggtc gcggagataa ccgatgacgg ccggtgtggt cagccccgcg aacgtcccgc
     9001 gcagcgcctc cggcacgtcg ggccggcgca tggtgaaacc ccgcacatgg agctcgtaga
     9061 tgaccgtgtc ctgccagggc cgttgcggcg gccggtcgtc gccccaggtg aaggccggat
     9121 cgacgacgcg gcatttcggc acgaaagccg cgctgtcgcg cgtgtcgaag gagagatcgg
     9181 cgtccgggct cgccgtgtca tagccgaaga cggcgtcgtt ccacgcgaac tgcccctcca
     9241 gcatgcgcgc ataaggatcg atcagcagct tgtgatggtt gaagcggtgg ccgcgctcgg
     9301 gcacataggg gccgaagacg cggtagccgt agagctgacc cggcacgacg tccggcaggt
     9361 agccgtgcca gacgtcgttc gtgtactcgc ggaggtcgat gcgatcggtc tccgactcgc
     9421 cggacgggtc gaagaggcac aattccaccc gctcggcgtt ctctgaaaag agggcgaaat
     9481 tcacgccctt gccgttccag gtcgcgccga gcggaaacgg ctctcccggc caaacacggg
     9541 tccgctgccc tttcgtcatg caggtcctat gcgtctaggc gtttcgcggg agagc
//
LOCUS       DASYNS010000578         8774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5295990, whole genome shotgun sequence.
ACCESSION   DASYNS010000578 DASYNS010000000
VERSION     DASYNS010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5295990"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VGP50_17565"
     CDS             complement(<1..383)
                     /locus_tag="VGP50_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8029245.1"
                     /translation="MNGIAGKVALVSGGATSIGAAVARAFHGAGASVVIADIAEDAGR
                     ALAAALGPQALYQKTDVTSDADIAACVGAGVAAFGGIDFLVNGAVSYLDKGLQSSRAE
                     WLHSLDVNLVGAALLLNAVVPEMTHR"
     gene            complement(380..976)
                     /locus_tag="VGP50_17570"
     CDS             complement(380..976)
                     /locus_tag="VGP50_17570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEV8029246.1"
                     /translation="MRPRTVVLALHYQNEIVHPEGRIRLGIAAAAPERDAIIAAARRL
                     LDGARAQGVPVISVRIAFRPDYADIVANARIWRRVIADQALAEGSWGAEFYRGLGPAP
                     GELVVTHQRNNPFHRSALAEALTRHGAERLVIAGISTTYVVESAVRHASDEGYDVVVA
                     ADACSSATPAMHEASLAAMAQLATVATVDAILAELAPG"
     gene            complement(987..1523)
                     /locus_tag="VGP50_17575"
     CDS             complement(987..1523)
                     /locus_tag="VGP50_17575"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEV8029247.1"
                     /translation="MTLVLGRAEAAPGIDGREVEQFLYREAMLLDDKNWAAWLALYTE
                     DCFYWVPSVVGQKDPVDTVSLYAEDRQRLEMRILRIDHPRAFSQDFPTRMSHVVGNVM
                     RDPEDGQGHDGGLRPQADLVVRSALHILEFRRDEQRLFGGTQRHWLRRVGGDWKIALK
                     RIDLLNCDAPMEVLQLFL"
     gene            complement(1525..2832)
                     /locus_tag="VGP50_17580"
     CDS             complement(1525..2832)
                     /locus_tag="VGP50_17580"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV8029248.1"
                     /translation="MGRYDAATIRSLIEPDRVHRDIYIDPALFALEMERIFGRAWIYV
                     GHESQVKRPGDYIATSIGRQPVVMSRHEDGAVHVLFNRCGHRGAKVVGDREGHVKYFR
                     CCYHGWTYRTDGTLLAVPVRRGYDDTGFDQSDPRYGMMTVPRVEIYRGFVFASLSPTG
                     PDLADYLGDARSSIDDMVDRSPDGALEVVGGCFRVLFRANWKIFLENLNDTMHPMVVH
                     ESSVEAGATWAKEHLPPGAPDPLPVRFTRNNGFPYAFWEKLTLRAHENGHSFMGGIFP
                     PRRTDPVFQDYVAAMVRAYGETRTEAILAVDRHNTIFYPNVSFQSGYQQVRIIRPLAV
                     DRTQMDVYAFRLKGAPDEFYKHTLVYSNVVNSPSSIVMPDDHEAYNRVQEGLAAQASD
                     WVSLHRDAGRDRGEGEWTSATGTSELPMRNQYRSWVRYMAQEA"
     gene            3375..4460
                     /locus_tag="VGP50_17585"
     CDS             3375..4460
                     /locus_tag="VGP50_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029249.1"
                     /translation="MAITMMSWGRIFCIAPALLLPSGAARAEPPPTPAITAAFSPAGA
                     VVGATPVSLDGQAGAGDFVVHTSTFPDGSVHEFTVKADEGGAYTDGAFLLQQLGTYHD
                     VLRDQATGASTELSYAGAGDFAAAVDPPQQSLRAGEQARFTLTIGSLEGFAGAVQPLA
                     PNLSKVPGAVASWSAAAVTVPPGGSASLTLTLRTLVGILPGSYDLVFAARNGAVSHRL
                     DPVRLTVTGPAPNAITAAPDPGAASGSTTPIAHQGQGDFSVILDASSETVTRGQEAQV
                     GVRFESLEGFRGTVAPTTSDLSGIQGLAAAWSLSSVAVAPGAPGVAQLTVRTDAATPP
                     GTYRIVVLGSNDSVTRMAPSALSLTLK"
     gene            complement(4501..4956)
                     /locus_tag="VGP50_17590"
     CDS             complement(4501..4956)
                     /locus_tag="VGP50_17590"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV8029250.1"
                     /translation="MRHLQGIDMLGPESPGAPTSREAALVQTLQAILGAFNSQDLDRV
                     LGFFAHDCVLEMHYGTAAWGSRYVGKDAVRHGLRTYFNATPELHYGDDTHFVIGDLGV
                     SKWTLRATSAKGERIEARGCDFYTFRDGKIVEKDSYLKARDNHSPRTGR"
     gene            complement(5196..6566)
                     /locus_tag="VGP50_17595"
     CDS             complement(5196..6566)
                     /locus_tag="VGP50_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011391051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8029251.1"
                     /translation="MAERPLTRAGLDLFMQGDVAATCNALEIVMPERRASRAPVANRL
                     AGARRRAPDVRGALIAAVLAFGGAAVGPAMAQISDGVVKIGVLNDQSGPNADLGGPGS
                     VVAARMAAEDFGGTVLGKPIEIVFADHQNKADIGAAIARQWFDQDHVDMVIDFANSSV
                     ALAVEQLAKDKNRIAIANAVGTTDFTGKACTATEASWLYDSYALTTNVARQMLKQGLD
                     TWFFITVDYAFGHSLEADTTRAVREGGGRVLGDVHFPLNSNDFSSYLVQAKASGAKVV
                     AFAGSGADMVSATKQAHEFGLAEDGQRLVPLLVFINDVHSLGLAAAQGLTFVTGFYWD
                     RTEESRAWAKRFMAHRKTMPTMVHASVYSAVTHYLRAATAAGTDEALAVMAKMRALPV
                     DDFYARGAKLRIDGRLVHDMYLAQVKKPSESTGPWDYYKIVATIPGDEAFQSLAAGGC
                     PLAQAH"
     gene            6704..7174
                     /locus_tag="VGP50_17600"
     CDS             6704..7174
                     /locus_tag="VGP50_17600"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV8029252.1"
                     /translation="MIDILFVGIGWTDIALTLNRIAVGSFFMHSGYHKLFNAERHRTF
                     VDELKSLGVPALGFNQWWVPSVEFSAGVAVLIGLLAPLAALGLLVIIVIAMATSGRQR
                     IKLYNPIDRGDRVDDWLYLPETLYAFMLIIIVSAGAGPYSLDALILGLLNKHVA"
     gene            7214..7636
                     /locus_tag="VGP50_17605"
     CDS             7214..7636
                     /locus_tag="VGP50_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV8029253.1"
                     /translation="MIVSDIDDELILAARLLLATLFLIFGWRKLRDFSGTVSQMVQLG
                     APMPVLVAGVATFMELPVAFAIAVGAFTRPLALLMALYTLGTALIGHRYWTAEGADYV
                     DSMDSFYKNLSIMGGFLLLYITGAGKHSIDALYGIATP"
     gene            7762..8217
                     /locus_tag="VGP50_17610"
     CDS             7762..8217
                     /locus_tag="VGP50_17610"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV8029254.1"
                     /translation="MSQGQVEPKTYQMPPQDGITVAHFLTVADVERSLRFYATVFGSR
                     ILSRGDSKGAPGYLQIANTWLIVNVGGGPTPDKPSVTLSVPANPDSVNSFMNIRVADI
                     QACYELWRSRGAEFITEPKDKYGETRCYIRDPDGYIIEVGQSKPDFTYG"
     gene            complement(8398..>8774)
                     /locus_tag="VGP50_17615"
     CDS             complement(8398..>8774)
                     /locus_tag="VGP50_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029255.1"
                     /translation="VGGGLYALSLATGEVVWHRPHPGCGDIPGCSPAQSAAVTAIPGV
                     VFSGGVDGHLRAYAAGTGDIIWDVDTRGSYHTVNGLDAQGGSIDGPGAVIVAGMVYTN
                     SGYAFFGSMPGNVLLAFSVGGD"
BASE COUNT         1470 a         2889 c         2882 g         1533 t
ORIGIN      
        1 cggtgggtca tctcgggcac cacggcgttg agcagcagcg cggcgccgac gaggttgacg
       61 tcgagcgaat gcagccattc ggcgcggctc gattgcaacc ccttgtcgag atagctcacg
      121 gcgccgttga cgagaaagtc gatgccgccg aacgccgcga cccccgcgcc gacgcaagcc
      181 gcgatgtccg cgtcgctggt gacgtcggtc ttctggtaga gcgcctgcgg cccgagcgcc
      241 gccgcgagcg cccggccggc gtcctcggcg atgtcggcga tgacgacgct cgcgccggcg
      301 ccatggaagg cgcgcgccac cgccgcgccg atgctggtgg caccgccgga gacgagcgcg
      361 accttgccgg cgatgccgtt catcccggcg ccagctccgc caggatcgcg tcgacggtcg
      421 cgaccgtggc cagctgcgcc atggccgcga ggctcgcctc gtgcatcgcg ggcgtcgccg
      481 acgagcaggc atcggccgcc accaccacgt cgtagccctc gtccgaagca tggcgcaccg
      541 cgctctcgac gacataggtc gtagagatgc cggcgatgac gaggcgctcg gccccatggc
      601 gcgtcagcgc ctcggcgagc gccgagcggt ggaacgggtt gttgcgctga tgcgtgacga
      661 cgagctcgcc cggcgccggg ccgagccccc gatagaattc ggcgccccag cttccttcgg
      721 ccagcgcctg atcggcaatc acgcgccgcc agatccgggc gttcgccacg atgtcggcat
      781 agtccggccg gaaggcgatg cgcacggaga tgacgggcac gccctgggcg cgggcgccgt
      841 cgagcagccg ccgcgccgcg gcgatgatgg cgtcgcgctc gggcgccgcc gccgcgatgc
      901 cgaggcggat gcgtccttcg ggatggacga tctcgttctg gtaatggagg gcgaggacca
      961 ccgtccgcgg acgcatcggc gctccttcag agaaagagct gcagcacttc catgggcgcg
     1021 tcgcaattga ggaggtcgat gcgcttcagc gcgatcttcc agtcgccgcc gacgcggcgc
     1081 agccaatggc gctgcgtgcc gccgaagaga cgctgctcgt cgcggcggaa ctcgaggatg
     1141 tgcagcgcgg agcgcaccac cagatccgcc tgcggccgaa gcccgccgtc atggccttgg
     1201 ccatcctcgg gatcgcgcat gacgttgccg accacatggc tcatgcgcgt cgggaagtcc
     1261 tggctgaagg cgcgcggatg gtcgatgcgg agaatgcgca tctcgaggcg ctggcgatcc
     1321 tccgcataga gcgagaccgt gtcgacggga tccttctgcc cgaccacgga cggcacccaa
     1381 tagaagcaat cctcggtgta gagcgcgagc cacgccgccc agttcttgtc gtcgagcagc
     1441 atcgcctcgc gataaaggaa ctgctcgacc tcgcgcccgt cgatccccgg cgccgcttcg
     1501 gctctgccca ggacgagcgt catgtcaggc ctcctgcgcc atgtagcgga cccagctccg
     1561 gtactggttg cgcatcggca gctcgctggt gccggtggcg ctcgtccatt cgccctcgcc
     1621 ccggtcgcgg ccggcatcgc gatgcaggct cacccagtcg gaggcttgcg cggcgagccc
     1681 ttcctggacg cggttatagg cctcgtgatc gtccggcatg acgatcgagc tcggcgagtt
     1741 caccacattc gaatagacga gcgtgtgctt gtagaactcg tccggcgcgc ctttgaggcg
     1801 gaaggcatag acgtccatct gcgtgcggtc gacggcgagc gggcggatga tgcgcacctg
     1861 ctgatagccg gattggaagg agacattcgg atagaagatc gtgttgtgcc ggtcgacggc
     1921 gagaatcgcc tccgtgcgcg tctcgccata ggcccggacc atcgccgcga catagtcctg
     1981 gaacacggga tcggtgcggc gcggcggaaa gatgccgccc atgaagctgt gcccgttctc
     2041 atgcgcccgg agcgtgagct tctcccagaa ggcataggga aaaccgttgt tgcgcgtgaa
     2101 gcggaccggc agcggatcgg gcgcgcccgg gggcaaatgc tccttggccc aggtcgcgcc
     2161 ggcctcgacc gagctctcat ggacgaccat cggatgcatg gtgtcgttga gattctcgag
     2221 gaagatcttc caattcgcgc ggaagaggac gcggaagcag ccgcccacca cctcgagcgc
     2281 gccgtcgggc gagcgatcga ccatgtcgtc gatcgagctg cgggcatcgc cgagataatc
     2341 ggcgagatcg gggccggtcg gactcaagct cgcgaagacg aagccgcgat agatctcgac
     2401 ccgcggcacc gtcatcatgc cgtagcgcgg atcgctctga tcgaagccgg tgtcgtcata
     2461 gccgcggcgc accggcaccg ccagcagcgt cccgtcggtg cgataggtcc agccgtgata
     2521 gcagcagcgg aagtatttca catggccttc gcggtcgccc accaccttcg cgccgcgatg
     2581 gccgcatctg ttgaagagca catggacggc gccgtcctcg tgccggctca tcaccaccgg
     2641 ctggcggccg atcgacgtcg cgatgtagtc gccgggcctc ttcacctggc tctcatgccc
     2701 gacatagatc caggcgcgac cgaagatgcg ctccatctcg agcgcgaaga gcgccggatc
     2761 gatgtagatg tcgcgatgga cgcgatcggg ctcgatgagc gagcggatcg tcgcggcgtc
     2821 gtagcgcccc atgggcccct ctccccagcc tcccccggcc ccccgggagg atgtgccgtg
     2881 ccggcccgcc ttctctaggg tcgcgacgac agccgcgtca actccggcgc gacgccgtag
     2941 gcgtcgtcgc gggcgggcgc cagcggttgc gcagcaaccg cgagagcccg cgcgtcgtcg
     3001 catcaaaccc gcttggcatt ggagcgctga ggagcggggg cagtttcgcc gacaatgatg
     3061 cttccgggcg atcggccctt gtgtcaggaa ctcgctttcg cgacatcggc gtgagcggca
     3121 acccgtctcg gcgatcacaa gactgtgagg tcacgcaccg cgccggcccc cggctgatgc
     3181 ggacggcacg cgcgcgttgt tcgttaggat gccatccgcc tgcgcgtctt gaccttcaat
     3241 atccagaacg cgcagggcga ccgcgacatg acgttgaatt ggatcgtcgc gcgggacgtg
     3301 gccgctccag cctttcggcc gccggatcgg cgcgcggcgc ggcctcgcat ggcgacagat
     3361 ggaggagagc cgacgtggcg atcacgatga tgagctgggg ccggattttt tgcatcgcac
     3421 cggcgctgct gctgccgtcc ggcgcagccc gcgccgaacc gccgccgacg cccgccatca
     3481 cggcggcgtt ttcgcccgcc ggcgcggtcg tcggcgcgac tccggtcagc ctcgacggac
     3541 aggccggtgc cggcgacttt gtcgtccaca cctcgacctt tccggacggc agcgtccacg
     3601 aattcacggt gaaagccgat gagggcggcg cctatacgga cggcgctttc ctgctgcagc
     3661 agctcggtac ttatcatgac gtgctccgcg atcaggcgac gggcgcatcg acggagctca
     3721 gctatgcggg cgccggggac ttcgccgcgg ccgtcgatcc gccgcagcag tcgctgaggg
     3781 cgggcgagca ggcgcgcttc acgctcacga tcggcagcct tgagggcttt gccggcgccg
     3841 tgcagccgct ggcgcccaat ctctctaagg tgcccggcgc cgttgcctcg tggtcggcgg
     3901 cggcggtgac ggtgccgccg ggcggctcgg cctcgctcac gctcacgctg cgcacgctgg
     3961 tcggcatttt gcccggcagc tacgacctcg tcttcgcggc ccgcaacggc gccgtcagcc
     4021 atcgcctcga tcccgtccgg ctgacggtga cgggtcctgc gccgaacgcg atcacggcgg
     4081 cgcccgaccc cggcgcagcg agcggctcga cgacgccgat cgcgcatcag gggcagggcg
     4141 atttcagcgt catcctcgat gcgtcgagcg agaccgtcac gcgcggtcag gaggcgcaag
     4201 tcggggttcg cttcgagagc ctcgaaggct tccgcggcac cgtcgcgccg acgacctcgg
     4261 acctttccgg catccagggc ctcgcggcgg cttggtcgct ctcatcggtc gccgtggcgc
     4321 cgggcgcgcc gggcgtcgcg cagctcaccg tcaggaccga cgcggccacg ccgcccggaa
     4381 cctatcggat cgtcgtcctc ggcagcaacg actcggtgac gcgcatggcg ccctccgcgc
     4441 tcagcctcac gctgaaatag cgtccggcca gcgatggcgt gtcctcctgc agatccgcga
     4501 tcaccgtccc gtcctcgggc tatggttgtc gcgggccttc aagtaggagt ccttctcgac
     4561 gatcttgccg tcgcggaagg tgtagaagtc gcagccgcgc gcttcgatgc gctcgccctt
     4621 ggccgatgtg gcgcgcaagg tccacttcga cacgccgagg tcgccgatga cgaaatgcgt
     4681 gtcatcgccg tagtgaagct cgggcgtggc gttgaaatac gtgcgcaagc cgtgtcgcac
     4741 cgcgtccttg ccgacatagc ggctccccca ggcagcggtc ccataatgca tctcgagcac
     4801 gcagtcatgg gcaaagaaac ccaggacgcg atcgaggtcc tggctgttga aggcgccgag
     4861 aatcgcctgg agcgtctgga ccagggcggc ctcgcggctc gtcggcgccc ccgggctctc
     4921 tggccccagc atgtcgatgc cctgcaggtg gcgcatgacc tcgcccaagc tgaatggtct
     4981 cggtttcccg gtcgtcatga ctggatttga ccccaatttc cttaacgaaa tttggccgct
     5041 cctccgcctt ctctgaggcg gcagtttccg ggatcgatga attgatcgac aaatgatcgg
     5101 aaaaactctc cttttccgaa gccggatcgc ttgagtcgcc acgatacccc tctccccttg
     5161 cgggagaggg gatttgtcgc tcgacgtcct cagtcttaat gcgcctgggc gagcgggcaa
     5221 ccgccggcgg ccaatgactg gaaggcctcg tcgccgggga tggtggcgac gatcttgtag
     5281 tagtcccagg ggcccgtgga ttcggagggc ttcttcacct gcgccagata catgtcgtgg
     5341 acgagccgcc cgtcgatcct gagcttggcg ccgcgcgcat agaagtcgtc gaccggcagc
     5401 gcgcgcatct tggccatgac ggcgagcgcc tcgtcggtgc ctgccgccgt ggcggcgcgg
     5461 aggtaatgcg tcacggcgga atagacactc gcatgcacca tggtcggcat cgtcttgcgg
     5521 tgcgccatga agcgcttggc ccaggcgcgg ctttcttcgg tccggtccca gtaaaagccg
     5581 gtgacgaagg tcaggccctg cgccgccgcg aggccgaggc tgtggacgtc gttgatgaag
     5641 acgaggagcg gcaccagccg ctgcccgtcc tcggcgaggc cgaactcatg cgcctgcttc
     5701 gtggcgctga ccatgtcggc gccgctgccg gcgaatgcca cgaccttggc gccggatgcc
     5761 ttcgcctgga ccaggtacga gctgaaatcg ttgctgttca agggaaagtg cacgtcgccc
     5821 agcacccgtc cgccgccctc gcgcacggcg cgcgtcgtgt cggcctcgag cgaatgaccg
     5881 aaggcgtagt cgacggtgat gaagaaccag gtgtcgagcc cctgcttcaa catctggcgt
     5941 gcgacattcg tggtgagcgc atagctgtcg tagagccagg acgcctcggt ggccgtgcac
     6001 gccttgccgg tgaagtcggt cgtgcccacg gcgttggcga tggcgatgcg attcttgtcc
     6061 ttggcgagtt gctcgaccgc gagcgccacc gatgaattgg cgaagtcgat gaccatgtcg
     6121 acatggtcct ggtcgaacca ttggcgggcg atggcggcgc cgatatcggc cttgttctga
     6181 tggtcggcga aaacgatctc gatgggcttg cccagcacgg tgccgccgaa atcctccgcc
     6241 gccatgcgcg cggccacgac ggaaccgggc ccgccgagat cggcgttggg ccccgactgg
     6301 tcgttcaaga cgccgatctt gacgacgccg tccgagattt gtgccatggc cggccctacc
     6361 gccgcgccgc cgaacgccag gacggcggcg atcagcgcgc ccctgacatc cggcgctcgc
     6421 cgtctcgcgc cggcgagccg gttggcgacg ggagcgcgtg acgcacggcg ctcgggcatg
     6481 acgatctcga gcgcgttgca ggtcgccgcc acatcgcctt gcataaagag atcgagaccg
     6541 gcgcgcgtca acggccgctc ggccatggct ccctcccccg tcggcgccca aatcttgcac
     6601 gccgcccgcc gaaggagcaa gaatcacgcg cgccgctcgg agcggcgcgg gcacgaggcg
     6661 ccgtaattcc ccgtttatag cgatgaagga aagaacagca gcgatgatag atatactgtt
     6721 cgttggtatc gggtggaccg acatcgcgct tactctcaac cgaatcgcgg ttgggtcatt
     6781 ctttatgcat tcgggatatc acaagctgtt caacgcagag cgccaccgta catttgtcga
     6841 tgaactcaag tcgctcggcg ttccagcact gggcttcaat caatggtggg tgccgtcggt
     6901 cgaattcagc gccggcgtcg ccgtccttat cgggctgctg gcgccactcg ccgcgcttgg
     6961 cctccttgtc atcatcgtca tcgccatggc gacgtccggg cggcagcgaa tcaaactcta
     7021 caacccgatc gatagaggag atcgcgttga cgattggctc tatttaccgg agacgcttta
     7081 tgcctttatg ttgatcataa tcgtttcggc cggcgccggg ccttacagcc tcgatgccct
     7141 gatcctcggc ctgttgaata agcacgtcgc ctgaccgcag gctcaccggt cgctctgttc
     7201 cgcgagattt cttatgatcg taagcgacat cgacgatgaa ctcattcttg cggcccgtct
     7261 tcttctcgcg acgttgtttc tgattttcgg ttggagaaag ctgagggatt tttcgggcac
     7321 ggtgagtcag atggtgcagc ttggcgctcc gatgccggta ctggttgctg gcgtagcgac
     7381 cttcatggag cttccggttg cgttcgcgat cgctgtcggc gcgttcacac gccccttggc
     7441 gctactcatg gctttgtaca cgttgggaac ggcgcttatt gggcatcgct attggacggc
     7501 ggaaggtgcg gattacgtcg acagcatgga cagcttctac aaaaatctca gcataatggg
     7561 aggcttcttg ctgttgtaca ttactggtgc gggaaaacat tcgatcgatg cattgtacgg
     7621 catcgccacg ccataagttt caaaagtcgc tctcctctga atcatgacac gagctcgctc
     7681 cggctcgaaa gcgacctttg ctggcaccgg aagaaccgcc taagctcgcg ttggatgagc
     7741 cgaccaagat aggagccgtc gatgagccaa ggacaagtcg agccgaaaac ctatcagatg
     7801 ccgccgcagg acggcattac cgtcgcacat tttctcaccg tcgccgacgt cgagcgatcg
     7861 ctgcgatttt acgcaacggt cttcggtagt cgcattctga gcagaggcga cagcaagggc
     7921 gcgccgggtt acctgcagat cgcgaacacg tggctcattg tcaacgtcgg gggcggcccg
     7981 acacccgaca aaccatcggt aacgctcagc gtccccgcca accccgattc ggtcaacagc
     8041 tttatgaata tccgcgttgc cgatattcaa gcctgctatg aactatggcg aagccgagga
     8101 gccgagttca tcacagagcc aaaggacaag tacggcgaga cgcgctgcta cattcgcgat
     8161 cctgatggct atattataga agttgggcaa agcaagccgg atttcactta cggctgaacg
     8221 ggctaggaac cgtcgattgc ggtttcgcgt gccatacgat agtggcgtcg aaagattaca
     8281 ttttccactc gtaccagaag cggtgagtcg ccacaggatt gttatcacgg ccgtccatcg
     8341 ctgcaaaatc gcggcaacgc gcgcgacggg ccatgcgtcc gagcggcgcg cggcccatca
     8401 gtccccgccg acggagaaag ccagaagcac gttgccgggc atcgagccga aaaatgcata
     8461 gcccgagttg gtgtagacca tgccggcaac gatgacggcg cccggtccgt cgatcgagcc
     8521 gccctgggcg tcgagcccgt tcaccgtgtg ataggagccc cttgtatcga catcccagat
     8581 gatgtcgccg gtgccggcgg cataagcgcg aagatgcccg tcgacgccgc cggagaagac
     8641 gacgcccggg atcgccgtta ctgccgccga ttgcgccggg ctgcagcccg gaatgtcgcc
     8701 gcagcccgga tgcggccggt gccagaccac ctctcccgtc gccaggctga gcgcgtagag
     8761 cccaccgccc acct
//
LOCUS       DASYNS010000579        10594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5608605, whole genome shotgun sequence.
ACCESSION   DASYNS010000579 DASYNS010000000
VERSION     DASYNS010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10594
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5608605"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VGP50_17620"
     CDS             complement(<1..432)
                     /locus_tag="VGP50_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008615129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV8029256.1"
                     /translation="MGRKLSIAALGAALAGGLFLRPAAAEPIKIGIILTYSGPNASLG
                     DQIDKGLRLYVKEHEKDLPPGTTVELVRRDDTGPNPDVARRLAQELVTRDHVQFLTGV
                     VWSPNAGAIAPIATEAKVPFIISNAAQAGLTRASPEIARVSF"
     gene            736..1614
                     /locus_tag="VGP50_17625"
     CDS             736..1614
                     /locus_tag="VGP50_17625"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEV8029257.1"
                     /translation="MIPGRFEYHRPASLGEAIALLAQHGEESRVLAGGHSLIPMMKLR
                     LAQPSHLVDLQSIAGLKGIREEGGAIVIGAMTTQAELLASALVAAKAPVLHEAAKLIA
                     DPQVRYCGTIGGNVANGDPGNDMPALMMALGASYVAQSAKGERRLDPRQYYQAPFQTV
                     LAEGEILTAIQVPAWPKGHGYAYEKMKRKVGDYATAAAAVLLEMSGGNCKRAAIALTN
                     VAGTPIYAAAAAKALEGSRVAAADIARAGDAAMAASDPVSDLRGPVEFRRHVTGVVTR
                     RAIARAVERAGQGGRS"
     gene            1611..2105
                     /locus_tag="VGP50_17630"
     CDS             1611..2105
                     /locus_tag="VGP50_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV8029258.1"
                     /translation="MSKKVVVSLTVNGEPVEALVEPRLLLIHLLRDQLNLTGPHVGCD
                     TSHCGACTVDMNGKSVKSCTIFAVQAEGAEVATIEGLAVGGQLHALQEGFAECHGLQC
                     GFCTPGMIMRAFRLLQENPDPSDEEIRWGLSGNLCRCTGYQNIVKAVRFAAAKLNSAK
                     EAAD"
     gene            2105..4510
                     /locus_tag="VGP50_17635"
     CDS             2105..4510
                     /locus_tag="VGP50_17635"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEV8029259.1"
                     /translation="MTAIPVPSSAERSDRLCGIGHSKRRKEDARFIQGKGNYVDDIKL
                     PGMLYGDLVRSPYGHARIKSINIEKAKALPGVRAVLTANDLKPLKLHYMPTLAGDVQA
                     VLADEKVLFANQEVAFIVADDRYIAADAVELVEVEYEQLPAVNNPEKALAPNAPVLRE
                     DLAGKTEGAHGKRRHHNHIFTWDVGDKDATDAAFAKAEVTAKEKIVYQRVHPAPLEPC
                     ACIGAMDKVKGELTVYGTFQAPHVIRTVLSILSGLAESKIHVISPDIGGGFGGKVGVY
                     PGYVCAIVASIVLGRPVKWVEDRIENLSTTAFARDYHMTGELAATKDGRITALRVHAY
                     ADHGAFDSCADPTKFPAGFFNIVTGSYDIPAAHCVVDGVYTNKAPGGVAYRCSFRVTE
                     ASYLIERMIDVLARKLKMDPAELRLKNFIRAEQFPYHSALGWEYDSGNYQPALKKAMD
                     AVDYAGLRREQAEKRKVGELMGIGIATFTEIVGAGPTRNCDILGIGMFDSCEIRVHPT
                     GSAIARLGTKSQGQGHETTYAQIIATELGLDSDNIQVEEGNTDTAPYGLGTYGSRSTP
                     VSGAATARCARKIRAKAQKIAAHLLEVGEGDLEWDVDRYRVKGLPERGVTMKEVAWAA
                     YNKPVPGLEPGLEAVDYYDPPNMTFPFGAYIVVLDIDRLTGESKVRRVYALDDCGTRI
                     NPMIIEGQVHGGLTEAFGIAMGQEIVYDDDGNVQGASFLDYFVPTAVETPKWETDFTV
                     TPSPHHPIGAKGVGESPNVGGVSAFSNAVIDAFAHLGVTHMPMPHTAWRVWQTCREHG
                     IGA"
     gene            4617..5495
                     /locus_tag="VGP50_17640"
     CDS             4617..5495
                     /locus_tag="VGP50_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV8029260.1"
                     /translation="MTDIDNLRTRFAAIGYVVDPELAAAVMLMQKLGRPLLLEGEAGV
                     GKTEVAKAMAAVLGAELIRLQCYEGLDASSAVYEWNYQRQLLAIKIWESENTSVAAKE
                     QHIFQREFLLERPLLRAITQEKPPVLLIDEIDRADDPFEAYLLEVLSDFQVSIPELGT
                     VKARSIPYVVLTSNGTRELSDALRRRCLYHYVDYPSEDKELRIIAARLPEMAPALAGQ
                     VVHFVQALRGEDLRKKPGIAETLDWARALIGLGASDLREDVEAVQQSLLCLLKTHEDR
                     AALPRERVERLLGKAG"
     gene            5492..6643
                     /locus_tag="VGP50_17645"
     CDS             5492..6643
                     /locus_tag="VGP50_17645"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV8029261.1"
                     /translation="MSGQRIERRIVGFVHLLRHNGFALGVEETLDALRVARAVDLERP
                     EALRWGLRALLASTEDEWRRFDELFDLHWRGRGVKQAVRSRGSGAGAFRGRKLGDAPP
                     GAPALADRVTRGDDGDASVGGEGKRQGASRAEALGSTDLRHINDPEELAQVARLAERL
                     ARRMQHRLSRRERVTRQGRRLDLRKTIHQSVRYGGTPMQIAFRARRREQLRLVLLVDV
                     SGSMSLYSTFFMRFVRGLVDRFRVAEAFVFHTRLVPVSPALRERDLARALDRMSLIAA
                     GWSGGTRIGESLAAFNRHYAADVLNSRSIVIIMSDGYDTGEPAALAAELRQLRRRARR
                     IVWLNPLLGWESYEPTAGGMAAALPYIDLFAPAHNLDSLAALEPELARL"
     gene            6656..7510
                     /locus_tag="VGP50_17650"
     CDS             6656..7510
                     /locus_tag="VGP50_17650"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEV8029262.1"
                     /translation="MNGDIEDVIDCMARMKGEEQPFAVATVVRSEGATAARVGAKAVV
                     RADGTMVGWVGGGCTLGAVKRAAANALVDGRARLIRVRPDAAGPSADGAEDFTNSCAS
                     GGTADIFIEPVAPRPALIILGASPTALALADLAKRLGFAVTAAAVSGDADDFAGADRR
                     LASFDLSAEPRASASYVVVATQGKRDRDALKAALATGAPYVAFVGSRRKAAQLAAELA
                     GDGVDRARLAALRSPAGIHIGAVTPAEIALSILTEIVAERRLGAGALRSEKEAQVTTA
                     TTVGSCCA"
     gene            complement(7567..7893)
                     /locus_tag="VGP50_17655"
     CDS             complement(7567..7893)
                     /locus_tag="VGP50_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029263.1"
                     /translation="MSFEPMQHEFTFDTNDISYRCIAKRSDRHRGHALEVGIFDHSGR
                     LVNCIVFCCHPEQADFNLCQDKSTEQLLDLAAEALRSGIYEEDLALVRPAGLRLYVRF
                     SDLSSP"
     gene            complement(7890..8807)
                     /locus_tag="VGP50_17660"
     CDS             complement(7890..8807)
                     /locus_tag="VGP50_17660"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8029264.1"
                     /translation="MPTDDMSASAIDNAALLERLAAANGIPYRPGAPPAPRRLRTPEG
                     LSLNLIDWGGDGAPILFLHGGSLTAHSWDLVCLALGDRFRCVALDLRGHGESDWAEVY
                     TIDACIADVAAIVAALGCSRVHVVGMSLGGNIAAHYAHAAGPRLASLTMVDVGPSVDF
                     AATATMRNFMDQPIAELTLDQLVDAALRASTRGGQDKILYRYRHLTQTTADGRLAWRQ
                     DRRKPHDYAHIFAKIQELDALARDIDCRVLLVRGALSRVWSDAAAADFTARFRDARWL
                     SIPDAGHNIPEDNPQTLAEALRTFLSEIA"
     gene            complement(8824..9828)
                     /locus_tag="VGP50_17665"
     CDS             complement(8824..9828)
                     /locus_tag="VGP50_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEV8029265.1"
                     /translation="MPKTNLQVLLASRPTGWASESNFRIAETPIPAVGDGQVLVKSHY
                     LSLDPYMRGRMNDSRSYAAKVEIGQVMGGEAVGEVVQSKNPQFKPGDTVLNRAGWQQY
                     GLSDGSDLRKVDPKAVPISTYLGVVGMPGVTAWMGLHDIGQPKPGETVVVSAASGAVG
                     SVVGQLAKARGCRVVGVAGGREKCDFVTKEFGFDACVDHRAGDLVKALAAAAPNGIDI
                     YFENVGGSLLDAVLALCNPFARIPLCGMISQYNAGDDRYGVKNLMAAVGMRIKLQGFI
                     VSDHMERWPEALKELSDGVRSGKIKYRETVTQGLENAPRAFIGLLKGENFGKQLVKVA
                     "
     gene            complement(9923..10447)
                     /locus_tag="VGP50_17670"
     CDS             complement(9923..10447)
                     /locus_tag="VGP50_17670"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV8029266.1"
                     /translation="MADAPGGRGPSVLTIPEGDNRERMVCPDCGFINYENPKVVVGAV
                     CLWEDRFLLCRRAINPRRGYWTLPAGYLELNESTPAGAIREAWEEAQAHITIDGLLAV
                     YDIPRISQVQLIYRAHLTAPEFAAGPESLEVKLFAWEEIPWDDLAFPSVRWALDHYRD
                     CAKTGDFTTRGTPG"
BASE COUNT         1750 a         3575 c         3581 g         1688 t
ORIGIN      
        1 gaaggagacg cgggcgatct cgggcgacgc gcgcgtcagg cccgcctgcg ctgcgttgct
       61 gatgatgaac ggcaccttgg cctcggtggc gatcggggcg atcgcgccgg cattgggcga
      121 ccagacgacg cccgtcagga actgcacgtg gtcgcgcgtc accagctcct gcgcgaggcg
      181 tctggcgaca tcggggttgg gcccggtgtc gtcgcgccgg acgagctcga ccgtggtacc
      241 gggcggcaga tccttctcgt gctctttgac atagaggcgg agccccttgt cgatctggtc
      301 gccgagcgaa gcgtttgggc cggaataagt gaggatgatg ccgatcttga tcggttccgc
      361 ggccgccggc cgaaggaaga gcccgccggc caacgccgcc cccagagcgg cgatcgacaa
      421 tttccgtccc atgatcgtcc accccctcgg gccgtgccgt ttctcaggct gtgccgcctg
      481 ttcatgccga ctatagcgat ggctaagcgg ccggagcaac gatcgggcgc gatcgggcgc
      541 gggattgtca ggcgcgccgc gcgttcgggc gcacccgccc acgcgtcgag cgatggcatg
      601 cactcatgac gcgtgccctg tcccgatccg ctcgcctcca tcgattcatt gacagccgcc
      661 gcactctccc ggagacttgg cggaagtaaa taaagacgtc cggttgaacg gtgcagccaa
      721 ggggaggaag ctcaagtgat accagggcgc ttcgaatatc atcgcccggc ctcgctcggc
      781 gaagccattg cgcttctcgc gcagcatggc gaggagagcc gggtgctggc cggcgggcac
      841 agcctcattc ccatgatgaa gctgcgcctc gctcagccgt cgcatctcgt cgacctgcaa
      901 tcgatcgccg gtctcaaggg catccgcgag gagggcggcg ccatcgtcat cggcgccatg
      961 accacccagg cggagctcct cgcctcggcg ctggtggcgg cgaaggcgcc ggtgctccat
     1021 gaggcggcaa agctgatcgc cgacccgcaa gtgcgctatt gcggcacgat cggcggcaac
     1081 gtcgccaatg gcgatcccgg caacgacatg ccggcgctga tgatggcgct gggagcgagc
     1141 tatgtggcgc aaagcgccaa gggcgagcgc cgcctcgatc cgcgccaata ctaccaggca
     1201 ccctttcaga cggtgctggc cgagggcgag atcctgacgg cgatccaggt gcctgcatgg
     1261 cccaagggcc atggttacgc ctatgagaaa atgaagcgca aggtcggcga ctatgcgacg
     1321 gcggcggcgg ccgtgctgct tgagatgtcc ggcggcaact gcaagcgcgc ggcgatcgcg
     1381 cttaccaatg tcgcggggac gccgatctat gcggccgcgg ccgcgaaggc actcgaaggc
     1441 agccgcgtcg cagcggccga catcgcgcgc gccggcgatg cggccatggc ggcgagcgat
     1501 ccggtctcgg atttgcgcgg cccggtcgag ttccgccgcc atgtgacggg agtggtgacg
     1561 cgccgcgcca tcgcccgggc cgtggagcgc gccggccagg gaggacggtc atgagcaaga
     1621 aagtggtggt gagcctcacc gtgaatggcg agccggtcga agcgctggtc gagccgcggc
     1681 tcctgctgat ccatctgctg cgcgatcagc tcaacctcac cggcccgcat gtcggctgcg
     1741 acaccagcca ttgcggcgcc tgcaccgtcg acatgaacgg caagtcagtg aagagctgca
     1801 cgatcttcgc ggtccaggcc gagggcgcag aggtcgcgac catcgaaggc ctggcggtcg
     1861 gcggccagct ccatgcgctg caggaagggt tcgccgagtg ccacggcctc caatgcggct
     1921 tctgcacgcc cggcatgatc atgcgcgcct tccggctgct ccaggagaat cccgatccca
     1981 gcgacgagga gatccgctgg ggcctctcgg gcaatctctg tcgctgcacc ggctatcaga
     2041 acatcgtcaa ggcggtgcgc tttgccgccg ccaagctgaa ctcggccaag gaggccgccg
     2101 actgatgacc gccatccccg ttccatcctc cgcggagcgc agcgatcgcc tctgcggcat
     2161 cggccattcg aagcggcgca aggaagacgc gcgcttcatc cagggcaagg gcaactatgt
     2221 cgacgacatc aagctgcccg gcatgcttta tggcgacctc gtccgcagcc cctatggcca
     2281 tgcccgcatc aagtcgatca acatcgagaa ggccaaggcg ctgcctggcg tgcgcgcggt
     2341 cctcaccgcc aacgacctga agcccttgaa gctccattac atgccgacgc tggccggcga
     2401 cgtgcaggcg gtgctcgccg acgagaaggt gctgttcgcc aaccaggaag tggcgttcat
     2461 cgttgccgac gaccgctaca tcgccgccga tgctgtcgaa ctggtcgagg tcgagtacga
     2521 gcagctcccc gcggtcaaca accccgaaaa ggcactggcg cccaatgccc cggtgctgcg
     2581 cgaggatctc gccggcaaga ccgagggcgc gcatggcaag cgccggcacc acaatcacat
     2641 cttcacctgg gacgtcggcg acaaggatgc gaccgacgcc gccttcgcca aggcggaggt
     2701 gacggccaag gagaagatcg tctatcagcg cgtccatccg gcgccgctcg agccctgcgc
     2761 gtgcatcggc gcgatggaca aggtgaaggg cgagctcacg gtctacggca ccttccaggc
     2821 gccgcatgtg atccgcacgg tgctgtccat tctgtcgggc ctggcggaat ccaagatcca
     2881 tgtgatctcg cccgatatcg gcggcggctt cggcggcaag gtcggcgtct atcccggcta
     2941 tgtctgtgcc atcgtcgcgt cgatcgtgtt gggccggccg gtgaaatggg tcgaggaccg
     3001 gatcgagaac ctgtcgacca cggccttcgc ccgcgactat cacatgaccg gcgagctggc
     3061 cgcgaccaag gacgggcgca tcacggcact ccgcgtccac gcctatgccg atcacggcgc
     3121 cttcgattcc tgcgccgacc cgacgaagtt cccggcgggc ttcttcaaca tcgtcaccgg
     3181 cagctatgac atcccggcag cgcattgcgt cgtcgacggc gtctacacga acaaggcgcc
     3241 gggcggcgtc gcctatcgct gctcgttccg cgtgaccgag gcgtcctatc tcatcgagcg
     3301 catgatcgac gtactggcgc gcaagctaaa aatggatccg gcggaactgc ggctcaagaa
     3361 cttcatccgg gcggagcagt ttccctatca ctcggcgctc ggctgggaat atgacagcgg
     3421 caattatcag ccggcgctca agaaggcgat ggacgccgtc gactatgcgg gcttgcgccg
     3481 cgagcaggcg gaaaagcgca aggtcggcga gctcatgggc atcggcatcg ccaccttcac
     3541 cgagatcgtc ggcgccgggc cgacgcgcaa ctgcgacatt ctcggcatcg gcatgttcga
     3601 cagctgcgag atccgcgtcc atccgacggg aagcgccatc gcgcgactcg gcaccaagag
     3661 ccaaggccag ggtcacgaga cgacctacgc ccagatcatc gccaccgagc tcggcctcga
     3721 ttccgacaac atccaggtcg aggagggcaa caccgacacc gcgccttacg gtctcggcac
     3781 ctacggctcg cgctcgacgc cagtgtcggg cgcagcaacg gcgcgatgcg cgcgcaagat
     3841 ccgcgccaag gcgcagaaga tcgccgcgca tctcctcgag gtcggcgagg gcgatctcga
     3901 atgggatgtc gaccgctacc gggtgaaggg cctgcccgag cgcggcgtca ccatgaagga
     3961 agtagcctgg gccgcctaca acaagccggt gcccggcctc gagcctggac tcgaggcggt
     4021 cgactattac gatccgccga acatgacctt tcccttcggc gcctacatcg tcgtcctcga
     4081 catcgaccgc ctcaccggcg agagcaaggt gcgccgcgtc tacgcgctcg acgattgcgg
     4141 cacgcgcatc aatccgatga tcatcgaggg ccaggtgcat ggcgggctca ccgaggcctt
     4201 cggcatcgcc atgggccagg agatcgtcta cgacgacgac ggcaacgtgc agggcgcgag
     4261 cttcctcgac tacttcgtgc cgaccgccgt cgagacgccg aaatgggaga ccgatttcac
     4321 cgtgacgccg tcgccgcatc atccgatcgg cgccaagggc gtcggcgaat cccccaatgt
     4381 cggcggcgtc tccgctttct cgaacgccgt catcgacgcc ttcgcccatc tgggcgtgac
     4441 gcacatgccg atgccgcaca cggcatggcg ggtctggcag acctgccgcg agcacggcat
     4501 cggcgcgtga cgcgaagcgt catcgcgggc gacgcccgtc gtctcccacc tcatcctgag
     4561 tccttctccg ccctcaaggg cggagaagga cttaatccga gtgcggcgga gctctagtga
     4621 ccgacatcga caatctccgc acgcgctttg ccgcgatcgg ctatgtcgtc gatcccgagc
     4681 tcgcggcggc ggtgatgttg atgcagaagc tgggccggcc gctgctgctc gagggcgagg
     4741 cgggcgtcgg caagaccgag gtggcgaagg cgatggccgc ggtgctgggc gccgagctca
     4801 ttcgcctcca atgctatgag ggcctcgacg cgtcgagcgc cgtctatgaa tggaactatc
     4861 agcgccagct tctcgccatc aagatctggg aaagcgagaa caccagcgtc gccgccaagg
     4921 agcagcacat cttccagcgc gagttcctgc tggagcgccc gctgctgcgc gccatcacgc
     4981 aggagaaacc gccggtgctg ctgattgacg agatcgaccg cgccgatgat cccttcgagg
     5041 cctatctcct cgaagtcctt tcggatttcc aggtgagtat ccccgagctc ggcacggtca
     5101 aggcgcggtc catcccctac gtcgtgctga cctcgaacgg cacgcgcgag ctctcggacg
     5161 cgctcaggcg ccgctgcctc taccactacg tcgactatcc ctcggaagac aaggagctgc
     5221 gcatcatcgc cgcgcggctt cccgaaatgg cgccggcgct tgccggccag gtcgtgcatt
     5281 tcgtgcaggc gctgcgcggc gaggacctgc gcaagaagcc gggcatcgcc gagacgctcg
     5341 actgggcgcg cgcgctcatc ggcctcggcg cctccgatct ccgcgaggat gtcgaggcgg
     5401 tgcagcagag cctgctctgc ctgctcaaga cgcatgagga tcgcgccgcg ctgccgcgcg
     5461 agagggtcga gcgactgctc ggcaaggccg gatgagcggc cagcgcatcg agcgccgcat
     5521 cgtcggattc gtgcatctcc tgcgccacaa cggcttcgcg ctcggcgtcg aggagacgct
     5581 cgacgcgctg cgcgtcgccc gcgccgtcga tctcgagcgt cccgaagcgc tgcgctgggg
     5641 cctgcgcgcg ctgctcgcct cgaccgagga cgaatggcgc cgcttcgacg agctgttcga
     5701 tctccattgg cgcggccgcg gcgtgaagca ggccgtgagg tcgcggggca gcggcgcggg
     5761 cgctttccgc ggccggaagc ttggcgatgc gccaccgggc gcgccggcgc tggccgatcg
     5821 cgtgacgcgg ggcgatgacg gcgacgcgtc ggtgggcggc gagggcaagc ggcagggcgc
     5881 gagccgcgcc gaggcgctgg gctcgaccga tctgcgccat atcaacgatc ccgaggagct
     5941 ggcgcaggtc gcgcgcctcg ccgagcgcct cgcgcggcgc atgcagcatc gcctgagccg
     6001 gcgcgagcgc gtcacccggc agggccgccg cctcgatttg cgcaagacga tccaccagag
     6061 cgtgcgttat ggcggcacgc ccatgcagat cgccttccgc gcgcgccgcc gcgagcagct
     6121 gcggctggtg ctgctggtcg atgtcagcgg ctccatgagc ctctacagca cgttcttcat
     6181 gcgcttcgtg cgcggcctcg tcgaccgctt ccgcgtcgcc gaagctttcg tcttccacac
     6241 gcgcctcgtg ccggtgagcc cggctttgcg cgagcgcgat ctcgcccgcg cgctcgaccg
     6301 catgtcgctg atcgccgccg gctggtcggg cggaacgcgc atcggcgagt cgctcgccgc
     6361 cttcaaccgg cattatgccg ccgatgtcct caattcccgg tcgatcgtga tcatcatgag
     6421 cgacggctat gacaccggcg agcccgcggc gctggcggcc gagctgcggc agctgcggcg
     6481 tcgcgcgcgc cgtatcgtct ggctcaaccc gctgctcggc tgggagagct acgagccgac
     6541 ggcgggaggc atggcggcgg cgctgcccta tatcgacctt ttcgcgcccg cgcacaatct
     6601 cgacagcctc gcggcgctcg agcccgagct ggcgcggctc taggagcacg gaagcatgaa
     6661 cggcgacatc gaggacgtga tcgactgcat ggcccgcatg aagggcgagg agcagccttt
     6721 cgccgtcgct accgtcgtgc gcagcgaagg cgccacggct gcccgcgtcg gcgccaaggc
     6781 ggtggtgcgc gccgatggca cgatggtcgg ctgggtcggc ggcggctgca cgctcggtgc
     6841 agtgaagcgc gccgcggcca atgcgctggt cgacggccgc gcccgcctca tccgcgtgcg
     6901 ccccgacgcc gccggcccgt ccgcggacgg tgcggaggat ttcaccaaca gctgcgcctc
     6961 cggcggcacg gccgacatct tcatcgagcc ggtggcgccg cgccccgcgc tcatcatcct
     7021 gggcgcgtcg cccaccgcgc tggcgctcgc cgatctggca aagcgcctgg gcttcgccgt
     7081 gaccgccgcg gcggtatcgg gcgacgccga cgatttcgcc ggcgccgacc ggcggctcgc
     7141 gagctttgat ctctcggcgg agccgcgcgc gagcgcgagc tacgtcgtgg tggcgacgca
     7201 aggcaagcgc gaccgcgacg cgctcaaagc cgcgctcgcc accggcgcgc cctacgtcgc
     7261 cttcgtcggc agccgccgca aggcggcgca gctcgcggcg gaactcgcgg gcgatggcgt
     7321 cgaccgtgcg cggctggcgg cgctgcgctc accggccggc atccatatcg gcgcggtgac
     7381 accggcagag atagcgctct cgatcctgac cgagatcgtc gccgagcggc ggctcggcgc
     7441 tggcgccttg cgcagcgaga aagaggcgca ggtcacgaca gcgacgacgg tgggaagctg
     7501 ttgtgcctag attagataat ggaacccttg gacagccggc tcggccctgt ctcaccgatc
     7561 gaagcgctac ggcgacgaca gatctgaaaa cctgacgtag agccgcaaac cggctggacg
     7621 caccagggcg agatcttctt catatatccc cgacctcagc gcttccgcgg cgaggtcaag
     7681 cagttgctcc gtgctcttgt cttgacagag attgaaatcg gcttgttcgg ggtggcaaca
     7741 gaagacgatg cagttcacca atctgccgct atgatcgaag atgccgacct cgagcgcatg
     7801 cccccgatga cggtctgaac gcttcgcgat gcagcggtag cttatatcgt tcgtgtcgaa
     7861 tgtgaattcg tgttgcatcg gctcaaagct catgcgatct cactcagaaa ggttctgagc
     7921 gcctccgcca gtgtctgcgg attgtcctcg gggatgttgt gaccggcgtc ggggatcgaa
     7981 agccagcgcg cgtcgcgaaa gcgcgccgtg aaatcggccg cggcggcgtc gctccagacc
     8041 cgactgagcg cgccccgcac caggaggact cggcagtcga tgtcgcgcgc cagcgcgtcc
     8101 agctcctgaa tcttggcgaa gatgtgtgca tagtcgtgcg gtttccgccg gtcctgacgc
     8161 caggcgagcc ggccgtcggc ggtcgtctgc gtcaggtggc gatagcgata aagaatcttg
     8221 tcttgcccgc cgcgcgtgct ggcgcgcagc gcggcgtcga ccaattgatc gagcgtcagc
     8281 tcggcgatcg gctgatccat gaaattccgc atcgttgccg tcgccgcgaa atcgacgctc
     8341 ggtccgacat cgaccatggt caggctggca agccgcggac cggccgcgtg cgcataatgc
     8401 gcggcgatgt tgccgccgag cgacatgcct acgacatgga cgcggctgca gccgagcgca
     8461 gcgacgatcg ctgcgacgtc cgcgatgcag gcatcgatgg tgtagacctc ggcccagtcg
     8521 ctctcgccat ggccgcgcag gtcgagcgcc acgcagcgaa agcggtcgcc gagcgcgagg
     8581 cacacgagat cccagctatg ggcggtgagc gagccgccat ggaggaagag gatcggcgcg
     8641 ccgtcgccgc cccaatcgat gaggttgagg ctcaagcctt ccggcgttcg cagccgccgc
     8701 ggcgcgggtg gtgcgccggg ccggtaggga atgccattcg ctgcggcgag ccgctcgagc
     8761 agcgccgcgt tgtcgatcgc gctggcgctc atgtcgtccg tcggcatgcc gggtcgcggc
     8821 gggctacgcc actttcacca gttgcttgcc gaaattctcg cctttcaaaa ggccgatgaa
     8881 cgcgcgcggc gcgttctcga ggccttgggt cacggtctca cggtatttga tcttgccgct
     8941 gcgcacgccg tcgctcagct ctttcagcgc ttcgggccag cgctccatgt ggtcggagac
     9001 gatgaagcct tgcagcttga tgcgcatccc gaccgcagcc atcagattct tgacgccgta
     9061 gcggtcgtcg ccggcgttgt attgcgagat catgccgcag agcggaatcc gcgcgaaagg
     9121 gttgcacagc gccagcacgg cgtcgagcaa ggacccgccg acattctcga aatagatgtc
     9181 gatgccattg ggcgccgccg ccgccagcgc cttgacgagg tcgccggcgc gatgatcgac
     9241 gcaggcgtcg aagccgaatt ccttcgtcac gaaatcgcat ttctcgcgcc cgccggcgac
     9301 gccgacgacg cgacagccgc gggccttcgc caattgcccg acgacgctgc cgacggcgcc
     9361 cgaggccgca gagacgacga cggtctcgcc gggcttcggc tggccgatgt cgtgcagtcc
     9421 catccaggcg gtgacgccgg gcatgccgac gactcccaga tatgtcgaga tcggcacggc
     9481 tttggggtcg actttgcgca ggtcactgcc gtcggaaagc ccatattgtt gccaaccggc
     9541 gcggttcagc acggtgtcgc cgggtttgaa ttgcgggttc ttcgactgga cgacctcgcc
     9601 caccgcctcg ccgcccatga cttggccgat ttccaccttc gccgcatagg agcggctgtc
     9661 gttcatccgc ccgcgcatgt agggatcgag cgaaagatag tggctcttca ccagcacctg
     9721 gccgtcaccc accgccggga tcggcgtctc ggcgatgcgg aaattgctct cgctggccca
     9781 gccggtcggc cggctggcga gcaggacctg tagattcgtc ttgggcattc ttgtctccct
     9841 ggggcgtggc tggcgatcct aacgcggctt gagcgccgcc gtcgaccgac gggacaacat
     9901 ggcagccggc cagatctgtc cgtcagcccg gcgtgccgcg cgtcgtgaag tcgcccgtct
     9961 tggcgcagtc gcggtagtgg tcgagcgccc agcgcaccga ggggaaggcg aggtcgtccc
    10021 aggggatctc ctcccaggcg aagagcttga cctcgaggct ctcgggcccg gccgcgaatt
    10081 ccggcgccgt caaatgggcg cgatagatga gctgcacttg gctgatgcgc ggaatatcgt
    10141 agaccgcgag caggccgtcg atggtgatat gcgcctgcgc ctcttcccag gcctcgcgga
    10201 tggccccggc cggcgtcgat tcattgagct cgaggtaccc ggccggcaag gtccagtagc
    10261 cacgccgcgg attgatggcg cggcggcaca gcaggaagcg gtcttcccac aggcacacgg
    10321 cgccgaccac gactttcggg ttctcgtaat tgatgaagcc gcaatcgggg cagaccatgc
    10381 gctcgcgatt gtcgccttcg gggatggtca gaacactggg gccgcggcca cccggagcat
    10441 cggccatggc gtcaaccgac gcggcgcggc agccgccgcg ctagaccgac agatcgagga
    10501 gacgtccgcg gtggtaggtg ggtcgggcga agcggccgtg atccgacgcg agattggacc
    10561 ccgagccgtg gcgggcgggc gtgacacgcc ggcc
//
LOCUS       DASYNS010000580         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5141645, whole genome shotgun sequence.
ACCESSION   DASYNS010000580 DASYNS010000000
VERSION     DASYNS010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5141645"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VGP50_17675"
     CDS             <1..1226
                     /locus_tag="VGP50_17675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8029267.1"
                     /translation="YLLVYGTLSYWLRKNGVSRSDIGYFALVGISYSFKFVWSPLIDR
                     LRIPVLTRRLGQRRSWALAIQLALMVAIYALGSTDPKMSLLSTALFAATVAFLSASQD
                     IVIDAYRIELLREEEQGAGAAVTQWGYRLGTLAGSAGAFDIAEFGGWHLAYTVMALLM
                     IVGIVAVLLTREPTVRPAVLRPEPLAATRGESLSLWVRGAVIEPFLDFMRRRAWLAIL
                     VLIVLYKLGEAFAASMSGTLYQELGFVEWEVGNIGKMVGLIASITGVGVGGLMVARFG
                     LFRALLFAGTLQMLGNLLYVALVLAGHNDVLLGASIFGENFTGAMASAAFVAYLSSLC
                     SPGFTATQYALYSSLAAVPARFLSAPSGWLVDRIDWIPFFLVATLACVPSLLLLWWMG
                     RRGDIDASPARAIAE"
     gene            complement(1233..>1925)
                     /locus_tag="VGP50_17680"
     CDS             complement(1233..>1925)
                     /locus_tag="VGP50_17680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEV8029268.1"
                     /translation="PTQLAHSSGKVPVRVRVSRRARRLALRIDAVADAVELVLPPRTS
                     LPRALNFLKSNRDWLETRLAALPPRIVLCDGERVPVFGLPHRIRHIARARGHGPVWIE
                     GQEIRVTGDPAHVARRVRDFLKEKARDELGRRARRLALQLGRKVGRISVRDTTTRWGS
                     CSANGNLAFSWRLIMAPEAVLHYVVAHEVAHLVEMNHGPRFWKLVDRLVPNVDRQRDW
                     LNRNRAWLLRIG"
BASE COUNT          262 a          653 c          666 g          344 t
ORIGIN      
        1 catacctgct cgtctacggc accttgtcgt attggctgcg caagaacggc gtatcccgct
       61 ctgacatcgg ctatttcgcg ctggtcggca tctcctacag cttcaaattc gtctggtcgc
      121 cgctcatcga ccgtctgcgc atcccggtgc tgacgcgccg cctcggccag cggcgaagct
      181 gggcgctcgc gattcaactc gcgctgatgg tcgcgatcta tgcccttggc agcaccgatc
      241 caaaaatgag ccttctgtcg acggcgctct tcgcggccac ggtcgcgttc ctctcggcca
      301 gtcaggacat cgtcatcgat gcctatcgca tcgagcttct tcgcgaggag gagcagggcg
      361 cgggggccgc ggtcacccag tggggctatc ggctcggcac gctcgccgga agcgcgggag
      421 cgttcgacat cgccgagttc ggcggctggc accttgccta taccgtcatg gcgctgctca
      481 tgatcgtcgg catcgtcgcg gtcctgctca cgcgtgagcc gacggttcgc cccgctgtcc
      541 tgcggcctga gccgcttgcg gcgacgcgcg gcgagagcct ttccctatgg gtgcgcggcg
      601 cggtgatcga gccctttctc gatttcatgc ggcgccgcgc ctggctcgcg atcctggtgc
      661 tcatcgtgct ctataagctc ggcgaggcat tcgccgcctc gatgtcgggc acgctttatc
      721 aggaactggg cttcgtcgaa tgggaggtcg gcaacatcgg caagatggtc ggcctgatcg
      781 ccagcatcac cggcgtcggc gtcggcggcc tgatggtggc gcgcttcggc ctgttccgcg
      841 cgctgctctt cgccggcacg ctgcagatgc tggggaatct gctttacgtc gcgctggtgc
      901 tcgccgggca caacgacgtc ctgctcggcg cctccatctt cggcgagaac ttcaccggag
      961 cgatggcgtc cgccgccttc gtcgcctatc tctccagcct ctgcagcccg ggtttcaccg
     1021 ccacccaata cgcgctctac tcctcgctcg ccgccgtgcc cgcgcggttc ctctcggcgc
     1081 cgagcggctg gctggtcgac cggatcgatt ggattccgtt ttttctcgtc gcgacgctcg
     1141 cctgcgtccc gagcctgctg ctgctgtggt ggatggggcg gcgcggcgac atcgatgcga
     1201 gccccgcccg ggcaatcgcc gaatgacggc gcctagccga tccgcagcag ccaggcgcgg
     1261 ttgcggttga gccagtcgcg ctggcggtcg acattgggga cgaggcggtc gaccagcttc
     1321 cagaagcgcg gcccgtggtt catctcgacg agatgcgcca cctcatgggc gacgacgtaa
     1381 tggagcaccg cctcgggggc catgatgagg cgccaggaaa aggccaaatt gccgttggcc
     1441 gagcagctgc cccagcgcgt cgtggtgtcg cggacgctga tccggccgac cttgcggccg
     1501 agctggagcg cgaggcggcg ggcgcggcgg ccgagctcgt cccgcgcctt ctccttgagg
     1561 aaatcgcgca cccggcgcgc cacatgggcg ggatcgcccg tgacgcggat ctcctgcccc
     1621 tcgatccaca ccggaccgtg gccgcgggca cgggcgatat ggcggatgcg gtggggcagc
     1681 ccgaagaccg gcacccgctc gccgtcgcag aggacgatgc ggggcgggag ggcggcgagg
     1741 cgcgtttcta gccagtcgcg attagatttc aggaaattca acgcccgcgg gagggaggtg
     1801 cgcggcggca ggaccagctc gacggcgtcg gccacggcgt cgatgcgcaa ggccaggcgg
     1861 cgggcgcggc ggctcacccg gacgcggacc ggcaccttgc cggaggagtg ggcgagctgc
     1921 gtcgg
//
LOCUS       DASYNS010000581         7498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5609109, whole genome shotgun sequence.
ACCESSION   DASYNS010000581 DASYNS010000000
VERSION     DASYNS010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5609109"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VGP50_17685"
     CDS             complement(<1..1035)
                     /locus_tag="VGP50_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029269.1"
                     /translation="MRSNAVQSLAGTLRPLQILLAAVVVIPLALFAGAAVLNYGWTWQ
                     EAGARLERTADTVDEHALKVFETNELILDRVAEQIGHLDWSEIDGSAQVHDYLKRLAQ
                     RVPHIGAVGLTGPDGRIVNSSLDIPSPPAFGNHRSFATMPRDGDDDILVSEATAGING
                     DEREFIVARYKPGTPHVADTGLIFVAVRSEYFRGYYKDAFDNQGYAINLLRDDGAILA
                     RYPSLPGDAVLSPKSGFRNAIAARPEQGTYSSVSEVDHKARSFAYRKLGAYPLYIVVG
                     LDRDAVIGTWWRKMASHLIFGIPATLCLVAITLLALSRTRRAQTLLAQANEEAARRER
                     LEASLYQAQKM"
     gene            complement(1111..1566)
                     /locus_tag="VGP50_17690"
     CDS             complement(1111..1566)
                     /locus_tag="VGP50_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEV8029270.1"
                     /translation="MVIRVQREDFDVGAELAALTRGKTGIGGVASFIGLVRDVAGGEA
                     VGAMTLEHYPGMTERQIEAIAAEARQRWPLEAVLIIHRYGRLEAGDRIVLVATASAHR
                     EAAFASCAFLIDWLKTKAPFWKLEETPKGARWVEAEASDDAAAARWTQG"
     gene            complement(1568..1819)
                     /gene="moaD"
                     /locus_tag="VGP50_17695"
     CDS             complement(1568..1819)
                     /gene="moaD"
                     /locus_tag="VGP50_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HEV8029271.1"
                     /translation="MTLLYFAWLRTRIGKAEERVEPPAGVTTVAALLDWLRSRGPAFA
                     EALKDTRILRVAVNQQYVGWNHPVRPGDEIALFPPVTGG"
     gene            complement(1889..3145)
                     /gene="glp"
                     /locus_tag="VGP50_17700"
     CDS             complement(1889..3145)
                     /gene="glp"
                     /locus_tag="VGP50_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEV8029272.1"
                     /translation="MAQLSDDCFAFGGELLSIGAALAMLDAKLVAVVEPETVPLKEAL
                     GRILAAAIDAPRNVPPYDNAAVDGYAVFFADLSPDAATELPIGGRAAAGHPLGRPARR
                     GEAIRIFTGAPMPEGPDTVLMQEDCSEAEGRVRILPGIKRGANRRRIGEDVMAGARVL
                     GSGRRLRPQELGLAAAMGFTTLPVYRRLRVAVFSTGDEVRDPGAALPAGAIYDANRYV
                     LYALLEGLGAAITDLGILPDRDSAIREALAAAAAAHDLVIGSGGMSTGEEDRVKAAVE
                     AQGRLHFWRLAIKPGRPVAMGQIGGIPFMGLPGNPVAVMVTFLLLARPLIQRLAGAAV
                     TPPQRFRVTAGFDYKKRSNRCEYVRARLVRDAAHGLRAEKFPRDGAGILSSMIESDGL
                     VEIGEGISRLAPGDPVEFLPFSELMG"
     gene            complement(3147..3668)
                     /gene="mobB"
                     /locus_tag="VGP50_17705"
     CDS             complement(3147..3668)
                     /gene="mobB"
                     /locus_tag="VGP50_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEV8029273.1"
                     /translation="MNHPDGTRIFGLAGWSGSGKTTLLAQLIPALTSRGVSVSTLKHA
                     HHAFDLDQPGKDSWRHRAAGASEVMVSSVNRWALIHENRGAPEAGFDELVRRMAPVDL
                     LLVEGFKDVPFAKLEVHRPVLGKPLLCRDNPHIVAVASDAPLSGVTVPVLALDDAAAI
                     ARFILAACRLEAA"
     gene            complement(3665..4288)
                     /gene="pgsA"
                     /locus_tag="VGP50_17710"
     CDS             complement(3665..4288)
                     /gene="pgsA"
                     /locus_tag="VGP50_17710"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV8029274.1"
                     /translation="MLTTLPNLLTLSRIVAIPLVVITFYLPSPYGPWIGCGLFALAGF
                     TDWLDGRLARLWQQQSELGRFLDPIADKLLVAAVLFMLAALQLREDPPPGKLVLAILF
                     VLPALVILSREILVSGLREHLAGLRVSVPVSRLAKWKTGLQMGAIGVLLVGDAGPSWL
                     PVTVIGELMLWAAAVLTMVTGYDYLRAGLTHINGEPSPVQAKPSSVA"
     gene            complement(4343..6334)
                     /gene="uvrC"
                     /locus_tag="VGP50_17715"
     CDS             complement(4343..6334)
                     /gene="uvrC"
                     /locus_tag="VGP50_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV8029275.1"
                     /translation="MSEQQQNDPAGEIVAAPKGGLAHGLGVIREAVRSLPAGPGVYRM
                     LDAKGDALYVGKARSLKSRVANYTHPANLSNRLRRMVAETRSVEVAMTHTEVDALLLE
                     NNLIKRLKPRYNVSLRDDKSFPYIHLTGSHDFPKITKHRGARGDPGEYFGPFASAGAV
                     NRTLIALQRAFLLRSCSDSVFASRTRPCLLYQIKRCSGPCVGRIDQPGYDRLVNEAKG
                     FLSGRNKEVQEQLAKRMQELSDKMEFESAAFVRDRIRALTQVQGRQDISIDAVGDADV
                     IAAHQAGGHTCIEIFFFRAGQNWGNRAYFPSHDRQMPVEEVLDSFLGLFYENKPPPRV
                     ILLSHEPTDCALIEEALSMRAGGKVVLSVPQRGEKAKLVEHALLNAREALGRRLAESA
                     TQQTLLEGVARAFGLEAPPQRIEVYDNSHIQGTHAVGAMIVAGPEGFMKSSYRKFNIR
                     GDDGGKRVDEGDLAAAIDAAAEPAKQPVTPDPLAPGDDYGMMREVLSRRFARALKEDP
                     ARDRGTWPDLLLIDGGAGQLSSVRQVLADLGIADIPVVAIAKGPDRDAGRERFFMEGR
                     PPFGLEPKDPVLYFLQRLRDEAHRFAIGSHRARRVKAIGTSPLDEIPGIGARRKRALL
                     HHFGSARGVARAGLADLERVDGISRAVAQKIYDHFHAEG"
     gene            complement(6739..>7498)
                     /locus_tag="VGP50_17720"
     CDS             complement(6739..>7498)
                     /locus_tag="VGP50_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019463025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8029276.1"
                     /translation="TPKAALVTGAGRRIGRAIALRLAADGWAVAVHYRSARTDAEMLA
                     GEIDAFGGRAVALAADLTRESDVAELVARAAADLGPLGCLVNNASIFENDLALTVTRE
                     SWDRHIETNLRAPFVLMQEFARRLPETAAGAIINILDQRVWNLTPYFISYTLSKAGLW
                     ALTQSMALALAPRIRVNGIGPGPTLPSPRQTPEQFRRQSEVMPLQRGTSPEEIGDAVH
                     FILSAPSMTGQMIALDGGQHLGWAQPAGRPPVGE"
BASE COUNT         1205 a         2436 c         2686 g         1171 t
ORIGIN      
        1 catcttctgc gcctggtaga gcgacgcctc gaggcgctcg cgccgcgccg cctcttcatt
       61 cgcctgagcc agcaaggtct gggcacgccg ggtgcggctc aaggccagca acgtgatggc
      121 gacgaggcag agcgtggctg gaatgccgaa gatgagatgg ctcgccatct ttctccacca
      181 cgtgccgatc accgcgtcgc ggtcgagccc gacgacgata tagagcggat aggcgccgag
      241 cttgcggtag gcgaaagagc gcgccttgtg gtcgacctcg gagacgctcg aataagtgcc
      301 ttgctcgggt ctggcggcaa tcgcgttgcg gaagccgctc ttgggtgaaa gcacggcgtc
      361 gcccggcaag ctcggatagc gtgcgaggat cgcgccgtcg tcgcgcagca ggttgatcgc
      421 atagccctga ttgtcgaagg cgtctttgta atatccgcga aaatattccg agcggaccgc
      481 gacgaaaatc agccccgtgt ctgcgacatg cggcgtgcca ggcttgtaac gcgcgacgat
      541 gaactcccgc tcgtcgccgt tgattccggc ggtcgcttcg ctgacgagga tgtcgtcgtc
      601 gccgtcacgc ggcatcgtcg cgaagctccg atgattgccg aacgccggtg gcgacggaat
      661 gtccaggctg ctgttgacga tgcgcccgtc ggggccggtc aggcccacgg cgccgatatg
      721 gggcacgcgc tgcgccaggc gcttcaggta gtcgtgaacc tgtgccgagc cgtcgatctc
      781 ggaccaatcg agatggccga tctgctcggc gacgcggtcg aggatcaact cgttggtctc
      841 gaacaccttg agggcatgct catcgaccgt gtcggcggtt cgctcgagcc gcgccccagc
      901 ttcctgccag gtccagccgt agttgagcac ggcggcgcct gcgaagagag cgaggggaat
      961 gaccacaacg gcagcaagaa ggatctgcag cggtcgaagc gtgccggcca ggctctgtac
     1021 ggcgttcgag cgcaatgcta ctccggaagg acctcaaggg ggcgttcgcc agccaataat
     1081 atggccggtg ccgatcggcg gccagcaaaa ctagccctgt gtccagcgcg cggcggccgc
     1141 gtcgtcgctc gcctccgcct cgacccagcg cgcgcctttg ggcgtctcct cgagcttcca
     1201 gaagggcgct ttcgtcttta accaatcgat caggaacgcg cagctggcaa aggccgcctc
     1261 gcgatgcgcc gaggcggtgg cgacgaggac gatgcggtcg ccggcctcga ggcggccata
     1321 gcggtggatg atcagcaccg cctcgagcgg ccagcgctgc cgcgcttcgg cggcgatggc
     1381 ctcgatctgg cgctcggtca tcccggggta gtgctcgagc gtcatggcgc cgacggcctc
     1441 gccgcccgcg acgtcgcgca cgagaccgat gaagctcgcg acgccgccga tgcccgtctt
     1501 gccgcgggtg agcgcggcga gctcggcgcc gacgtcgaaa tcctcgcgct gcacgcggat
     1561 caccacgtca gcctccggtg accggcggga agagcgcgat ctcgtcgccc ggccgcaccg
     1621 ggtggttcca gccgacgtat tgctggttta ccgcgacgcg caggatccgc gtgtccttga
     1681 gcgcctcggc gaaagcaggc ccgcggctcc ggagccaatc gagcagcgcc gccacggtgg
     1741 tgacgccggc gggcggctct acccgctcct cggccttgcc gatcctggtg cgcagccaag
     1801 cgaaatagag cagcgtcatg acccagagct acgacttcgg cgaccgtatt tcaacccagc
     1861 tgagggcttt gccatccgtc ctccccggtc agcccatgag ctcgctgaag ggcaggaact
     1921 cgacgggatc gccgggcgcg aggcggctga tgccttcgcc gatctcgacg aggccgtcgg
     1981 actcgatcat cgaggagagg atgccggcgc cgtcgcgcgg gaacttctct gctcggagcc
     2041 cgtgggcagc atcgcggacg aggcgcgcgc gcacatactc gcagcgattg ctgcgcttct
     2101 tgtagtcgaa accggccgtc acgcggaaac gctgcggcgg tgtcacggcg gcgccggcga
     2161 ggcgctggat caaggggcgc gccagcagca ggaacgtgac catcacggca acgggattgc
     2221 cgggcaggcc catgaagggg atgccgccga tctgtcccat cgccacggga cgcccaggct
     2281 tgatggcgag gcgccagaaa tgcaggcggc cctgagcctc gaccgcggcc ttgacgcggt
     2341 cctcctcgcc cgtcgacatg ccgcccgagc cgatgacgag gtcatgagca gcggctgccg
     2401 cggcaagcgc ctcgcgaatc gcggagtcgc ggtcgggcag aatgccgaga tcggtgatgg
     2461 cggcgccgag gccttcgagc agcgcgtaga gcacatagcg gttggcatcg tagatcgcgc
     2521 ccgccggcag cgcggcgccg gggtcgcgca cctcgtcgcc ggtcgaaaaa accgcgacgc
     2581 gcaggcggcg gtagacggga agcgtcgtga agcccatcgc cgcagcgagg cccagctcct
     2641 gcggacgcag ccgccgcccg gagccgagca cgcgcgcccc ggccatcacg tcctcgccga
     2701 tgcggcggcg gttggcgccg cgcttgatgc cgggcaggat gcgcacgcgg ccctcggcct
     2761 cgctgcaatc ctcctgcatg agcaccgtgt ccggtccctc gggcatcggc gcgccggtaa
     2821 agatgcggat ggcctcgccg cgacgcgcgg ggcgtcccag cggatggccc gcggcagcgc
     2881 gcccgccgat cggcagctcc gtcgccgcat cgggcgaaag gtcggcgaag aacacggcat
     2941 agccgtcgac ggcggcgttg tcgtatggcg gcacgttgcg tggggcgtcg atggccgcgg
     3001 cgaggatgcg acccagcgct tccttgagcg gcacggtctc gggctccacc accgccacga
     3061 gcttggcgtc gagcatcgcg agcgcggcgc cgatcgacag cagctcgccg ccgaaagcga
     3121 agcaatcatc gctcagctgt gccatgtcag gcggcctcga gacggcacgc cgccaggatg
     3181 aaacgcgcga tcgccgcggc atcgtcgagg gcgagcaccg gcacggtcac gccggacaag
     3241 ggcgcatcgc tggcgacggc gacgatgtgc gggttgtcgc ggcagagcag cggcttgccg
     3301 agcaccgggc ggtgcacttc gagcttggcg aagggcacgt ccttgaagcc ttcgacgagc
     3361 aggagatcga ccggcgccat gcggcgcacg agctcgtcga aacccgcttc gggcgcgccg
     3421 cggttctcgt ggatgagcgc ccagcgattg accgaggaga ccatcacctc gctggcgccg
     3481 gcggcgcgat ggcgccagct gtccttgccg ggctggtcga gatcgaaggc gtgatgcgca
     3541 tgcttcagcg tcgagacgga gacaccgcgc gacgtgagcg cggggatcag ctgcgcgagc
     3601 agcgtcgtct tgccgctgcc gctccagccc gcgaggccga agatccgcgt cccgtccggg
     3661 tggttcacgc gacgctgctc ggctttgcct ggacaggcga gggctcgccg ttgatgtggg
     3721 tgagaccggc gcgcagatag tcgtagccgg tgaccatcgt cagcaccgcg gcggcccaca
     3781 gcatcagctc gccgatcacc gtcacgggga gccaggaggg cccggcgtcg cccaccagga
     3841 gcacgccgat cgcgcccatc tggagcccgg tcttccattt ggcgaggcgc gagaccggca
     3901 cactgacgcg caagcccgcg agatgctcgc gcaggcccga gacgaggatc tcgcggctca
     3961 ggatcaccag ggccgggaga acgaagagaa tggcgagcac gagcttgccg ggtggcggat
     4021 cctcgcgcag ctggagcgcc gccagcatga acagcacggc cgccaccaga agcttgtcgg
     4081 cgatcgggtc gaggaagcgc ccgagctccg attgctgctg ccagagccgc gcgaggcggc
     4141 cgtcgagcca atcggtgaag cccgccagcg cgaagaggcc gcagccgatc cagggcccat
     4201 agggcgaggg caaatagaag gtgatgacga ccagaggaat cgccacgata cgcgagagag
     4261 tgaggagatt ggggagcgtc gtcagcatgg tcgggaactg tcccggcaga gcaagagggg
     4321 atttccgctc agtctagcgc ctctagcctt ccgcatggaa atggtcgtag atcttctgcg
     4381 ccacggcgcg gctgatgccg tcgacgcgct cgagatcggc gaggccggcg cgcgccacgc
     4441 cgcgcgccga gccgaagtgg tgcaggagcg cgcgctttcg ccgggcgccg atgccgggga
     4501 tctcgtcgag cggcgacgtg ccgatggcct tgacccgccg ggcgcgatga ctgccgatgg
     4561 cgaagcgatg cgcctcgtcg cgcaagcgtt gcaggaaata gagcacggga tccttgggct
     4621 cgaggccgaa gggcggccgc ccctccatga agaagcgctc gcggccggca tcgcggtcgg
     4681 ggcccttggc gatcgccacc acgggaatat cggcgatgcc gagatcggcc aggacctgcc
     4741 gcaccgatga gagctggccc gcgccgccgt cgatgagcag gagatcgggc caggtgccgc
     4801 gatcgcgcgc cgggtcctcc ttgagcgcgc gggcgaaacg ccggctcagc acctcgcgca
     4861 tcatgccgta atcgtcgccc ggcgcgagcg ggtcgggcgt cacgggctgt ttcgccggtt
     4921 cggcggcggc atcgatcgcc gccgcgaggt cgccctcgtc gacgcgctta ccgccgtcgt
     4981 cgccgcggat gttgaacttg cgatagctgc tcttcatgaa gccttcgggg ccggcgacga
     5041 tcatcgcgcc cacggcgtgg gtcccctgga tgtggctgtt gtcatagacc tcgatgcgct
     5101 gcggcggcgc ctcgaggccg aaggcgcggg cgacgccctc gagcagcgtc tgctgcgtgg
     5161 cgctctcggc gagccgccgc cccagcgcct cgcgcgcgtt gagcagcgca tgctcgacga
     5221 gcttggcctt ctccccgcgc tgcggcaccg agagcacgac cttgccgccg gcgcgcatcg
     5281 agagggcttc ctcgatcagc gcgcagtcgg tcggctcatg gctgagcaga atgacccgcg
     5341 gcggcggctt gttctcgtag aagaggccga ggaaggagtc gagcacttcc tcgaccggca
     5401 tctgacggtc gtggctcggg aaataggcgc ggttgcccca gttctgcccg gcccggaaga
     5461 agaagatctc gatgcaggtg tggccgccgg cctgatgcgc ggcgatgacg tcggcgtcgc
     5521 cgaccgcgtc gatgctgatg tcctggcggc cctgcacctg cgtcagcgcg cggatgcggt
     5581 cgcgcacgaa ggccgcgctt tcgaactcca tcttgtccga gagctcctgc atgcgcttcg
     5641 ccagctgctc ctggacctcc ttgttgcggc ccgagagaaa gcccttggcc tcgttgacga
     5701 gacggtcata gccgggctgg tcgatgcggc cgacgcaggg cccgctgcag cgcttgatct
     5761 ggtagaggag gcagggccgc gtgcggctgg cgaaaacgct gtcgctgcag gagcgcaaca
     5821 ggaaggcgcg ctgcagcgcg atgagcgtgc ggttgacggc gccggccgag gcgaaggggc
     5881 cgaaatactc gccggggtcg ccgcgcgcgc cgcgatgctt ggtgatcttg gggaagtcgt
     5941 ggctgccggt gagatggata tagggaaacg acttgtcgtc gcggagcgag acgttgtagc
     6001 ggggcttcag ccgcttgatg aggttgttct cgagcaggag cgcgtcgacc tcggtgtggg
     6061 tcatcgcgac ctcgaccgag cgcgtctcgg cgaccatgcg gcgcagccga ttggaaagat
     6121 tggcgggatg ggtgtagttg gcgacgcggc tcttgaggct gcgcgccttg ccgacataga
     6181 gcgcatcgcc cttggcgtcg agcatgcggt agacgccggg accggccggc agcgagcgca
     6241 ccgcctcgcg gatgacgccg aggccgtgag cgagcccgcc tttcggcgcg gccacgatct
     6301 ctccggctgg gtcgttctgc tgttgctcgc tcatgccctt gacgtggctc aggacgcatc
     6361 gcgcgtcaac ggccgctccg gtcattccgg ttcgcgggat atccacggcc gttgtggaca
     6421 aatgtgttga aaacgctgcg tggccatggc gcctttcgcg gcaaacctat ggtttcgaca
     6481 ctttgcccaa attttgcgca aaattgctat cgtattgatt tagaatgata atattcttgt
     6541 caagtgtcaa tctatcgtaa caatggtccg tcggcggggg aaaactaggc aggaatttcg
     6601 ccgccgttgg tgctgtgaac aacttcggcc cgggcccttt gcgcgggcgg tcgcggctgc
     6661 cgtaaatctg acttgtttcg ggcatttagg ggtgctgctt aatctgttct gtgccgattg
     6721 atgacgcccg gaaaggggtc actcgccgac gggtggccgg cctgccggct gcgcccagcc
     6781 caggtgctgg ccgccatcga gtgcgatcat ctgtccggtc atggacggcg ccgacaggat
     6841 gaagtgcacg gcgtcgccga tctcctcggg gctggtgccg cgctggagcg gcatcacctc
     6901 gctctggcgg cgaaactgct cgggggtctg gcgcgggctc ggcagcgtcg gcccggggcc
     6961 gatgccgttg acgcggatgc gcggcgccag cgccagcgcc atgctctgcg tcagtgccca
     7021 gagcccggcc ttgctcagcg tgtaggagat gaaatacggc gtcaggttcc aaacgcgctg
     7081 gtcgaggatg ttgatgatgg cgcccgctgc cgtctcgggc aggcggcggg cgaattcctg
     7141 catcagcacg aaaggcgcgc gcagattggt ctcgatatgg cgatcccaac tctcgcgcgt
     7201 gacggtgagc gccagatcgt tctcgaagat ggaagcgttg ttgacgaggc aacccaaagg
     7261 gccgagatcg gcggcggcgc gcgccaccag ctcggcaacg tcgctctcgc gcgtcagatc
     7321 ggcggcgaga gccaccgcgc ggccgccgaa agcatcgatc tcgcccgcca gcatttccgc
     7381 gtcggtccgc gcggagcgat aatgcacggc gaccgcccag ccatcggcgg cgaggcgaag
     7441 cgcgatggcg cggccgatgc gccggccggc gccggtgacg agcgcggctt tcggggtt
//
LOCUS       DASYNS010000582         7058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_150479, whole genome shotgun sequence.
ACCESSION   DASYNS010000582 DASYNS010000000
VERSION     DASYNS010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150479"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1500)
                     /locus_tag="VGP50_17725"
     CDS             complement(169..1500)
                     /locus_tag="VGP50_17725"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEV8029277.1"
                     /translation="MGLGVALAPATVLAQVFTEPLVPVPPVPVSPAIPGAPPETPELQ
                     FLPRGFSVADRPRPELDPLGLQFGDFFFFPRAEVDEAYNDNIFATTTGKTSAFITTFA
                     PGFDLRSNLPQNAINVSAGGAFVRYDEHTGLDSDNGFGDVNGRLDVDNAHFFTGDLRV
                     ERSNITLGEPQVPGNAASPLKFNDYNATFGFDQYRLRIGYEVTATVRREEYEAIPLVG
                     GGSLPESNLNNFAYEAAIRPYYEFSPGYQAYLRVAYNRRDYDHGEGNGVPTLNSDGYR
                     VDVGARVDLTGVTYVDGFIGYLDQSYQAAQFGSIGGVDFGAKVVWNVTQLTSIGAYVG
                     RTVEDINTAVLAGVDNSPGFLATTAGVSIDHELLRNLLLNGTLAYTNDAFENITRTDN
                     VYSGTAGVKYLLNRHLYVGANYTYLRVNSNGQAAMIPFSQNIFLLRISTQL"
     gene            complement(1830..1994)
                     /locus_tag="VGP50_17730"
     CDS             complement(1830..1994)
                     /locus_tag="VGP50_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029278.1"
                     /translation="MYAHKLEREMPAAGLSMRKGDIRWPRYRRVAFLVTAASLCWAVP
                     VVVAYLIAAA"
     gene            complement(2084..3556)
                     /gene="gatB"
                     /locus_tag="VGP50_17735"
     CDS             complement(2084..3556)
                     /gene="gatB"
                     /locus_tag="VGP50_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV8029279.1"
                     /translation="MSTTTIEGRTGPWEVIIGLEAHCQVIAKAKLFSGAATEFGAEPN
                     TQVSLVDAAFPGMLPVINRCCVEQAVKTGLGLDAEINLVSIFDRKNYFYADSPAGYQI
                     SQYTRPIVGKGKITLDLPDGKSREVAITRLHLEQDAAKSLHDQHPSLTYVDLNRAGVA
                     LMEIVSEPDMRSAEEAGAYLRKLRSILRYLGTCDGNMEEGSLRCDCNVSVRKPGAPLG
                     TRCEIKNVNSIRFVMQAIEYEARRQIEVIEEGGTIAQETRLFDAGRGVTRPMRSKEQA
                     HDYRYFPDPDLLPLTLDAAWVEQLRRRLPELPDAKKARFVADYKLSPYDAGVLVGEQA
                     SADFFEAVAKGRDAKLAANWVMGDLFGALNRLGRGIEQSPLTAAALGELIDLIGDGTI
                     SGRLAKDVFAAMLETAKSAATLVAERGLRQVTDTGAIDSAIDAVLAANGDKVAEYRSG
                     KEKLFGFFVGQVMKATEGKANPGVVNELLRGRLAPPGRGK"
     gene            complement(3553..5088)
                     /gene="gatA"
                     /locus_tag="VGP50_17740"
     CDS             complement(3553..5088)
                     /gene="gatA"
                     /locus_tag="VGP50_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV8029280.1"
                     /translation="MTALTDLTIAEARQGLRGKQFSAREMALAHIGAMEAARPLNAFI
                     AETPERALEMAAASDRRLAKGEALALDGMPLAVKDLFCTEGVATTAGSRILGGFTPPY
                     ESTVTARLWQAGAVMLGKTNMDEFAMGSSNITSAYGKVESPWRRPGDNRALVPGGSSG
                     GSAAAVAARAAIAATGTDTGGSIRQPASFCGIVGLKPTYGRCSRWGIVAFASSLDQAG
                     PMTRSVRDAAIMLQTMAGHDPGDSTSAPLLVPDFEAALTGDIRGLKIGIPREYRVEGM
                     PAEIDALWERGIAWLKAAGAEPVEIGLKLTKYALATYYIIAPAEASSNLARYDGVRFG
                     LRVPGETLDQMYENTRGEGFGAEVRRRILIGTYVLSAGYYDAYYLKAQKVRALIARDF
                     AEAFARCDAILTPTAPSAAFAIGEKMDDPIAMYLNDVFTVPVNLAGLPGISVPAGLSA
                     ESLPLGLHVIGRAFDEETMLRVAGVLETAANFTARPQFPLPSREREGPVAKRREGEGA
                     RDA"
     gene            complement(5090..5377)
                     /gene="gatC"
                     /locus_tag="VGP50_17745"
     CDS             complement(5090..5377)
                     /gene="gatC"
                     /locus_tag="VGP50_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV8029281.1"
                     /translation="MALDKATVARIATLARIKVSASEQEHLATELTGILTWIEQLDEV
                     DTTGVEPMSSVAAMRLPMREDVVTDGNCRDKILSDAPAPAHGFFTVPKVVE"
     gene            5431..5907
                     /gene="ruvX"
                     /locus_tag="VGP50_17750"
     CDS             5431..5907
                     /gene="ruvX"
                     /locus_tag="VGP50_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV8029282.1"
                     /translation="MPIRNPSDLKSLIKKGERLLGLDVGTKTVGMALSDTRLIVATPL
                     DTIRRTRFRDDVKRLQAEIDRHGVGGIVVGLPLSLDGSEGPRAQSVRQFAQNLLAHRD
                     LPLAFWDERLSTAAVEREMIAADLSRKRRGEIIDRVAAAYILQGLLDRLAQPQGEN"
     gene            6036..>7058
                     /locus_tag="VGP50_17755"
     CDS             6036..>7058
                     /locus_tag="VGP50_17755"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HEV8029283.1"
                     /translation="MAAFIAVVCLALIGIEGWRVISARGTALRSGMVDAENLALSLSR
                     SVATTLRATDVVLRDLTERLENDGGPPQLARLHQLLKTNAAALPLIDDIGVFDAEGNT
                     VTSSLSNPDASNISDREYFAYHRSDADRGLHVGRPVQSRATGQWIVPLSRRVDGADGS
                     FAGIVRMAVSLEAWRAFYQVFEIGKSGAISVYLADGTLLVRRSDGASEVGRDFSKTTL
                     FRERLSEAPEGSFIDPSPHDGVVRLVSYRRVDGYPLVLDVALSQDEELAEWRDGARGH
                     FAADAVLAALLAALGIWLLRQARRRRIAEEASAAVAREYRLLADNSTDIVIAFGLEGR
                     TRYVSPA"
BASE COUNT         1174 a         2337 c         2361 g         1186 t
ORIGIN      
        1 cgcacagatc gggagtggca ctcgcggcga gagggctcgt cgtcataggt gcgggctctc
       61 gcggttgcaa gagcaaccgc tgccgcccgg tcgcgacgac gctgcgcgtc gcgcggggtt
      121 gcaagagcaa ccgctgccgc ccgccagcga cggccctgcg ggccgtcgct agagctgcgt
      181 gctgatacgc agcaggaaga tattctggct gaacgggatc atggctgcct gcccgttcga
      241 gttcacgcgc agataggtgt agttggcgcc gacatagagg tggcgattga gcagatactt
      301 caccccggcc gtgccgctgt aaacgttgtc ggtgcgcgtg atgttctcga acgcgtcgtt
      361 ggtgtaagcg agggtgccgt tgagcagcag gttgcgcagc agctcgtgat cgatcgaaac
      421 ccccgccgtc gtcgcgagga agccgggcga gttgtcgacg cccgcgagca cggccgtgtt
      481 gatgtcctcg acggtgcggc ccacatacgc gccgatcgag gtaagctgcg tgacgttcca
      541 cacgaccttc gcgccgaaat caacgccgcc gatgctgccg aattgcgccg cctggtagct
      601 ctgatcgaga tagccgatga agccatcgac ataggtgacg cctgtcagat cgacccgcgc
      661 gccgacgtcg acacgatagc cgtccgagtt cagcgtcggt acgccgttgc cctccccatg
      721 atcgtagtcg cggcggttgt aggcgacgcg cagataggcc tggtatcccg gcgagaactc
      781 gtaataggga cggatcgccg cttcataggc gaagttgttg aggttgctct ccggcaggct
      841 gccgcctccg accagcggga ttgcctcgta ctcctcgcgc cgcaccgtcg cggtcacctc
      901 gtagccgatg cggagccgat actggtcgaa gccgaaggtg gcgttgtaat cgttgaattt
      961 gagcggtgaa gccgcgttcc ccgggacctg cggctcgccg agggtgatat tcgagcgctc
     1021 gacacgaaga tcgccggtga agaaatgcgc gttgtcgacg tcgagccggc cgttcacgtc
     1081 gccgaagccg ttgtcgctgt cgaggccggt gtgctcgtcg tagcggacga aggcgccgcc
     1141 ggcgctgaca ttgatcgcgt tctgcggcag gttcgagcgc aggtcgaagc cgggggcgaa
     1201 ggtggtgatg aaggcgctgg tcttgccggt cgtggtggcg aagatgttgt cgttataggc
     1261 ctcgtccact tcggcgcgcg gaaagaagaa gaagtcaccg aactggaggc ccagcggatc
     1321 gagctccggc cgcggccggt cggccaccga gaagcctcgg ggcaggaact gcagctcagg
     1381 cgtctccgga ggagcgccag ggatcgccgg cgataccggc accggcggca ccggcaccag
     1441 aggctcggtg aagacctgcg ccagcaccgt cgccggggcg agcgcgacgc ccaagcccat
     1501 cagcgacgcc gctgtccggc cgatcccgga gggcgccggc cgccgacgcg gcaaaccaga
     1561 gcgtcctgca aggtcccacg cagaaattgt tttctcagcc attgctcatc gacccgtgat
     1621 tcgttgcgag cagcccaggg cctcaaccat tgcgcaacaa tcgtcgccgc tattgacgag
     1681 atagtaaacg caaatcgcga aacgcaaatc gcggtcgctt gtcgggcaat acccggcgat
     1741 ttacaacatc tctttcgtag acgtgctcga aagagtgagg tcccgcgcgg gacccattcc
     1801 gttgctatat tacggaaaaa cctagctttt caggcggcgg cgatgagata ggcgacgacg
     1861 accggcaccg cccagcagag cgaagccgcg gtcaccagaa aggcgacgcg gcgatagcgc
     1921 ggccagcgaa tatctccctt ccgcatggag agaccggcgg ccggcatctc cctctcgagc
     1981 ttgtgcgcgt acacggcggc ctccccacgg actttgattg taccgtcgta acatggcaaa
     2041 agttaacacg gggttagcca taaccccact ccccgaacca gtcctacttc ccccgtccgg
     2101 gaggcgcaag ccttcctcgc agcaattcgt tgacgacgcc cggattggct ttgccttccg
     2161 tcgccttcat cacctggccg acgaagaacc cgaagagctt ctccttgcct gaccggtatt
     2221 ccgcgacctt gtcgccgttg gcggcgagca ccgcgtcgat cgcgctgtcg atggcgccgg
     2281 tatcggtcac ctggcgcaag ccccgctccg cgacgagcgt cgccgcgctc tttgccgttt
     2341 ccagcatggc ggcaaagacg tccttggcga ggcgccccga aatcgtgcca tcgccaatga
     2401 ggtcgattag ctcgcccaac gccgccgcag tgagcggcga ttgctcgatg ccgcgcccga
     2461 gccggttgag cgcgccgaag aggtccccca tcacccagtt ggcggcgagc ttcgcgtcgc
     2521 ggcccttggc gaccgcttcg aagaagtcgg cgctcgcctg ctcgcccacg aggacgcccg
     2581 cgtcataggg cgagagcttg taatcggcga cgaagcgcgc ctttttcgca tccggcagct
     2641 cgggcagccg ccggcggagc tgctcgaccc aggctgcatc gagcgtcagc gggagcaggt
     2701 cgggatcggg gaaatagcgg taatcatgcg cctgctcctt ggagcgcatg ggacgcgtga
     2761 cgccgcgccc ggcgtcgaag agccgcgttt cctgcgcgat cgtcccgcct tcctcgatga
     2821 cctcgatctg gcggcgcgcc tcatactcga tcgcctgcat gacgaagcgg atcgagttga
     2881 cattcttgat ctcgcagcgc gtgccgagcg gcgcgcccgg cttgcggaca gacacgttgc
     2941 agtcgcaacg gaggctcccc tcctccatgt tgccgtcgca ggtgccgaga tagcgcagga
     3001 tcgagcgcag cttccgcaga taggcgccgg cctcctcggc gctgcgcatg tcgggctcgg
     3061 agacgatctc catcagggcg acgccggcgc ggttcagatc gacataggtg aggctcggat
     3121 gctggtcgtg caggctcttg gccgcatcct gctcgagatg gagacgtgtg atggcgacct
     3181 cgcggctctt gccgtccggc aggtcgaggg tgatcttgcc tttgcccacg atcggccgcg
     3241 tgtattgcga gatctggtag cccgcgggcg agtcggcgta gaaatagttc ttgcgatcga
     3301 agatcgacac gaggttgatc tcggcatcga gcccgagccc cgtcttcacc gcctgttcga
     3361 cgcagcagcg gttgatcacg ggcagcatgc cgggaaaggc cgcgtcgacg agcgagacct
     3421 gcgtgttggg ctcggcgccg aattccgtgg cagcgcccga gaacagcttc gccttggcga
     3481 tgacctggca atgggcctcg aggccgatga tgacctccca gggaccggtg cgcccttcga
     3541 tcgtcgtggt gctcatgcgt cgcgagcccc ctcaccctcc cgtcgcttcg cgacgggccc
     3601 ctccctctcc cgcgagggga gaggaaactg ggggcgcgcc gtgaagttcg ccgccgtctc
     3661 gaggacgccg gcgacgcgca gcatggtctc ctcgtcgaag gcgcggccaa tgacatgcag
     3721 gccgagcggc aggctctcgg cggaaaggcc ggcgggaacg gagatgccgg gcaggccagc
     3781 caggttcacc ggcaccgtga agacgtcgtt cagatacatg gcgatcggat catccatctt
     3841 ctccccgatc gcaaaggcgg ccgagggcgc ggtcggcgtc aggatcgcgt cgcaacgcgc
     3901 gaaggcttcg gcgaagtcgc gggcaatgag tgcacgcacc ttctgggcct tcagataata
     3961 tgcatcgtaa tagccggccg agagcacata ggtgccgatc agaatgcgcc ggcgcacctc
     4021 ggcgccgaag ccctcgccgc gcgtgttctc gtacatctgg tcgagggttt cgccgggcac
     4081 gcggaggccg aagcgcacgc cgtcatagcg cgcgaggttg gacgaggctt cggccggggc
     4141 gatgatgtag taagtggcga gcgcgtattt ggtgagcttc aagccgatct cgacgggctc
     4201 ggcgcccgcc gccttaagcc aggcgatgcc ccgctcccac agcgcgtcga tctccgccgg
     4261 catgccctcc acgcgatatt cgcgggggat gccgatcttg aggccgcgga tgtcgccggt
     4321 cagcgccgct tcgaaatcgg gcacgagaag cggcgccgag gtggaatcgc cgggatcatg
     4381 cccggccatc gtctgcagca tgatcgcggc atcgcgcacg cttcgcgtca tcggccccgc
     4441 ctgatcgagc gaggaggcga acgcgacgat gccccaacgc gagcagcggc cataggtcgg
     4501 cttcaagccg acgatgccgc agaagctcgc gggctggcgg atcgagccgc cggtgtcggt
     4561 gccggtggcc gcgatcgccg cgcgcgccgc gaccgccgcc gccgagccgc ccgacgagcc
     4621 gcccggcacc agcgcgcggt tgtcgcccgg ccgccgccac gggctttcca ccttgccata
     4681 ggcgctggtg atattggacg agcccatggc gaactcgtcc atgttggtct tgccgagcat
     4741 gaccgcgccg gcctgccaga gcctggccgt gaccgtcgat tcataaggcg gcgtgaagcc
     4801 gccgaggatg cgcgagccgg cggtggtggc gacgccttcc gtgcagaaga gatccttgac
     4861 ggcgagcggc atgccgtcga gcgcgagcgc ctcgcccttg gcaaggcgcc ggtccgatgc
     4921 ggccgccatc tcgagtgccc gctcgggcgt ctcggcgatg aaggcgttga gcggacgcgc
     4981 cgcctccatc gctccgatat gcgccagcgc catctcacgc gccgagaatt gtttcccgcg
     5041 aagaccctgc cgcgcttcgg cgatagtgag gtcggtgagc gccgtcatct cactcgacga
     5101 ccttgggcac ggtaaagaag ccatgcgccg gcgccggcgc gtcggacagg atcttgtcgc
     5161 ggcaattgcc gtcggtgacg acgtcctcgc gcatgggcag ccgcatcgcc gcaacgctcg
     5221 acatcggctc gacgccggtg gtgtcgacct cgtcgagctg ctcgatccag gtgagaatac
     5281 ccgtgagctc ggtcgcgaga tgctcctgct cgctcgcgga caccttgatg cgcgccagcg
     5341 tggcaatccg agccacggtg gccttgtcca atgccatgat gtagatgtct ccagccccgc
     5401 ggaaacctgc gaggctacgt agcaccgggc atgccgatcc gcaaccccag cgacctcaag
     5461 tccctgatca agaagggcga gcggctcttg ggcctcgatg tcggcaccaa gaccgtcggc
     5521 atggccttga gcgacaccag gctcatcgtc gccacgccgc tcgacaccat ccgccgcacc
     5581 cgtttccgcg acgacgtgaa gcggcttcag gccgagatcg accgccacgg cgtgggcggc
     5641 atcgtcgtcg gattgccgct gagcctcgac ggcagcgagg gcccgcgggc tcagagcgtg
     5701 cggcagttcg cccagaacct gctggcccat cgcgacttgc cgctcgcctt ctgggacgag
     5761 cggctctcga cggcggcggt cgagcgcgag atgatcgccg ccgacctctc gcgcaagcgg
     5821 cggggcgaga tcatcgaccg ggtggccgcc gcctatatcc tccagggctt gctggatcgc
     5881 ctagcacagc cgcaaggcga aaattaaccg gcccgtcgta tctagggaat gatgcgcccc
     5941 ctttcggggc catcttcagc ggaacctgtt cccgcatgga ccaattcaag attggcgccc
     6001 gcccgccgcg gcctcgcatt tccctcgagg tggccatggc agccttcatt gccgtcgtct
     6061 gcctggcgct catcggcatc gagggatggc gcgtcatctc ggcccgaggc accgcgctcc
     6121 gcagcggcat ggtcgatgcg gaaaatcttg cgctgtcgct cagccgcagc gtggccacca
     6181 cgctcagagc cacggacgtc gttctccgcg acctgaccga gcggctcgag aatgacggcg
     6241 ggccgcccca gcttgcgcgg ctccaccagc tcctgaagac gaatgccgcg gcactcccgc
     6301 tgatcgacga catcggcgtc ttcgatgccg aaggcaacac ggtgacgagc tcgctgtcga
     6361 atccggacgc gagcaacatc tccgatcgcg aatattttgc ctaccatcgc agcgatgccg
     6421 accgcggcct gcatgtcgga cgccccgtgc aaagcagggc aaccggccag tggatcgtcc
     6481 ccttgtcgcg gcgagtcgat ggtgcggacg gaagctttgc cggcatcgtg cggatggccg
     6541 tgagcctcga agcctggcgc gccttctatc aggtcttcga gatcggcaaa agcggcgcca
     6601 tcagcgtcta cctggccgac ggcaccctgc tggtccgccg gtccgacggc gccagcgagg
     6661 ttggccggga cttctccaag accacgcttt tccgcgaaag gttgtcggaa gcgcccgagg
     6721 gaagcttcat cgacccgagc ccgcatgacg gcgtcgtgcg cctggtcagc tatcgccgcg
     6781 tcgacggcta cccgctggtg ctcgacgtcg cgttgtcgca agacgaggag ctggcggagt
     6841 ggcgcgacgg tgcccggggc cattttgccg ccgatgccgt tctggccgcg cttctggcgg
     6901 cgcttggcat ctggcttctg cggcaggcgc ggcgccggcg cattgccgag gaggcgagcg
     6961 cggccgtggc gcgggaatat cgccttctcg ccgacaattc gaccgatatc gtcattgcct
     7021 tcgggctcga gggccggacg cgctacgtat cgccggcg
//
LOCUS       DASYNS010000583         3779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_307977, whole genome shotgun sequence.
ACCESSION   DASYNS010000583 DASYNS010000000
VERSION     DASYNS010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307977"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VGP50_17760"
     CDS             <1..948
                     /locus_tag="VGP50_17760"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV8029284.1"
                     /translation="YLGFAENDDHPPHDVMRGEKLLADVYNALRGNDALWQSTLLVVF
                     YDEHGGFYDHVAPPPAVPPDDHHDEYSFDRLGVRVPALLVSPWVDRRVEHTLFDHTSV
                     LRYLIDKWHLQPLGRRTAAAHSIAVALTRRAPRTDTVSRIVLTEEQLLAPDPELEDRL
                     FGIPDTHQTALRILADYLTREIVTDAPRIYSWLARLIEGLRSVCEGLLARLYGDPPAT
                     SISIAEPDRLALYGSAKPRDDVAHFLMHRKRQAVADLGAAIGDETLPEARVDHAVQTL
                     ALITGRPYHREKNGREHAKAWLSRHVSASATLRRRGRLR"
     gene            977..2656
                     /gene="ettA"
                     /locus_tag="VGP50_17765"
     CDS             977..2656
                     /gene="ettA"
                     /locus_tag="VGP50_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEV8029285.1"
                     /translation="MPAYQYIYVVKGLSKTYPGGREVLKDVWLSFLPGAKIGVLGPNG
                     AGKSTLLRIMAGLDQDFTGEAWAADGAKVGYLPQEPQLNPDKDVAGNVKEALGEITAL
                     MERFEAVSARFAEELGEDEMNALMVEQGEIQEKIDAVNGWELDRTIEIAMDALRCPPG
                     DADVTTLSGGERRRVALCRLLLQKPDLLLLDEPTNHLDAESVAWLQRFLAEYAGTVVT
                     VTHDRYFLDDVAEWILEVDRGRGIPYEGNYSGWLEQKQKRLEQEGKQEAARQRTLQHE
                     LEWVRQSPRARQAKSKARVSAYESLVAQSREKAPETAQIVLPPGPRLGDLVIEAENVA
                     KGYGDRLLIEDLSFRLPPGGIVGIIGPNGAGKTTLFRMITGQDKPDRGSFRVGETVKL
                     GYVDQSRDTLAPEKTVWQEISSGNDEIDLGRRKMPSRAYCSLFNFRGSDQQKKVGQLS
                     GGERNRVHLAKMLKSAANVILLDEPTNDLDVDTLRALEEALLEFPGCAVIITHDRWFL
                     DRIATHMVAFEGDSQVVWFEGNYADYEADRHRRLGAEADQPHRIRYKPLTR"
     gene            complement(2669..3611)
                     /locus_tag="VGP50_17770"
     CDS             complement(join(2669..3274,3276..3611))
                     /locus_tag="VGP50_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611132.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV8029286.1"
                     /translation="MAKPYSLDLRERVVAAVLGGASCREAARTFRVSIASVVKWSQRF
                     RATGSAAAKPMGRQQARSLAPQRAWLLARLEAAPDITLRALVAELGERGVVTSYGSVW
                     RVVHDAELSFKKTLFAAEQDRPDVARRRQRWKAYQGRVDPTRLVFIDETWAKTNMTPL
                     RGWAPRGRRLVGKVPQGRWRTVTFLAALRCDRIDAPCVIDGPIDGESFRAYVEQVLVP
                     TLKPGDIVIFDNLGSHKGKTVRAAIRAAGAKLFLLPKYSPDLNPIEQVFAKLKLLLRK
                     AAKRTTDALWHQIGALLANFSPSECANYVRNAGYASK"
BASE COUNT          684 a         1244 c         1214 g          637 t
ORIGIN      
        1 tatctcggct tcgccgagaa cgacgatcat ccgccgcacg atgttatgcg cggcgagaag
       61 ctgctggccg acgtctacaa tgcgctgcgc ggcaacgacg cgctctggca gtcgacgttg
      121 ctcgtcgtgt tctacgacga gcatggcggc ttctacgatc acgtggcgcc gccgccggcg
      181 gtgccgcccg acgatcatca cgacgagtac agcttcgacc gtctcggcgt gcgtgtgccg
      241 gcgctcctcg tctctccctg ggtcgatcgc cgcgtcgagc acacgctctt cgatcacacg
      301 agcgttctac gctacctcat cgataaatgg cacctgcagc cgcttggccg ccgcaccgcg
      361 gccgcgcaca gcatcgccgt ggcgctcacg cgccgcgcgc cgcgcaccga cacggtctcc
      421 cgcatcgtgc tgaccgagga gcagctgctt gcccccgatc cggaattgga ggaccggctc
      481 ttcggaatac cggacacgca ccagaccgcg ctccggatcc tcgccgatta tctgacgcgc
      541 gagatcgtca ccgacgcgcc ccggatctat tcgtggctgg cacggctgat cgagggccta
      601 agatctgtct gcgagggtct tcttgcgcgg ctctacggcg atccgccggc gaccagcatc
      661 tcgatcgccg agcccgaccg cctcgccctc tacggctcgg cgaagccgcg cgacgatgtc
      721 gcgcattttc tcatgcatcg caagcggcag gccgtcgctg atttgggcgc ggccatcggt
      781 gacgaaacgc tgccggaggc gcgcgtcgac cacgccgtgc agaccttggc gttgatcacc
      841 ggccgcccct atcatcgcga gaagaacggg cgcgagcatg caaaagcctg gctcagccgg
      901 catgtaagcg cctcggcgac attgcgccgg cgcggcaggc tgcgctagaa ggcgcggcac
      961 tcaggagccc cagccgatgc ccgcctatca gtacatctat gtggtgaagg gcctgtccaa
     1021 gacgtatccg ggcgggcgcg aagtgctgaa ggacgtctgg ctgtcgtttc ttccgggcgc
     1081 caagatcggc gtgctcggcc ccaacggcgc cggcaagtcc acgctgttgc gcatcatggc
     1141 ggggctcgat caggatttca ccggcgaggc ctgggcggcc gacggcgcca aagtcggtta
     1201 tctgccgcag gaaccgcagc tcaaccccga caaggacgtc gccggcaatg tcaaagaagc
     1261 gcttggcgag atcacggcac tgatggagcg attcgaagcg gtcagcgcgc gcttcgccga
     1321 ggagctcggc gaggacgaga tgaatgcgct catggtcgag caaggcgaga ttcaggagaa
     1381 gatcgacgca gtgaatgggt gggagctcga ccgcaccatc gaaatcgcca tggatgcgct
     1441 gcgctgcccg ccgggcgacg ccgatgtgac gacattgtcg ggcggcgagc ggcgtcgcgt
     1501 cgcgctctgc cggttgttgc tgcaaaaacc cgacctgctg ctgctcgacg agcccaccaa
     1561 ccatttggac gccgaaagcg tcgcctggct gcagcgcttt ctcgccgaat atgccggaac
     1621 cgtcgtcacc gtcacccatg accgctattt cctcgatgac gtcgccgagt ggattctcga
     1681 ggtcgatcgc ggccggggca tcccctacga aggcaattat tcgggctggc tcgagcagaa
     1741 acagaagcgg ctcgagcagg aaggcaagca ggaggcggcg cgccagcgaa cgctgcagca
     1801 cgagctcgaa tgggtgcgcc agagcccgcg cgcccgccaa gccaagagca aggcgcgcgt
     1861 ctccgcctat gaaagcctgg tggcgcagag tcgcgagaag gcgcccgaaa cggcgcagat
     1921 cgtgctgccg cccgggccgc ggctcggcga tctcgtcatc gaggccgaga acgtcgccaa
     1981 gggttatggc gatcgcctct tgatcgagga tctctccttt cgcctgccgc ccggcggcat
     2041 cgtcggcatc atcgggccga acggcgccgg caagaccacg ctcttccgca tgatcaccgg
     2101 ccaggacaag cccgaccgcg gcagcttccg cgtcggcgag acggtgaagc tcggctatgt
     2161 cgaccagtcg cgcgacacgc tggcgcccga gaagacggtc tggcaggaga tctccagcgg
     2221 caatgacgag atcgatctcg gccggcgcaa gatgccgagc cgagcctatt gctcgctctt
     2281 caattttcgc gggtccgatc agcagaagaa ggtcgggcag ctatcgggtg gcgagcgcaa
     2341 ccgcgtccat ctggcgaaga tgctgaagag cgccgccaat gtcatcttgc tggacgagcc
     2401 gaccaacgat ctcgatgtcg acaccctgcg cgcgctcgag gaggcgctcc tcgagtttcc
     2461 cggatgcgcc gtcatcatca cccatgaccg ctggttcctc gaccgcatcg ccacgcatat
     2521 ggtcgccttc gagggcgaca gccaggtcgt gtggttcgag ggcaactacg ccgactacga
     2581 agccgaccgc catcgccgcc tcggcgccga ggcggatcag ccgcatcgga tcaggtataa
     2641 accgctgacg cgctagagct tgatccacct actttgaagc gtatccggcg tttcggacat
     2701 agttggcgca ttcgcttggt gagaagttgg cgaggagagc gccgatctgg tgccagaggg
     2761 cgtcggttgt tcgcttggcg gccttgcgga gcaggagttt cagtttggcg aagacctgct
     2821 cgatggggtt gaggtctgga ctgtatttcg gcagcaggaa gagcttggcg ccagcggcgc
     2881 gaatggcggc acggacggtc ttgcccttgt ggctgccgag attatcgaag atgacgatat
     2941 cgccgggttt gagagttgga acgagcacct gttcgacata ggcgcgaaag ctctcgccgt
     3001 cgatcggccc gtcgatgaca cagggcgcat cgatgcgatc gcagcgcaag gcggcgagga
     3061 aggtcacggt tcgccatcgc ccctgtggca ccttgcccac gagccggcgg ccgcgcggcg
     3121 cccagccgcg gagcggcgtc atgttggtct tggcccaggt ctcgtcgatg aagacgagcc
     3181 gcgtcggatc aactcgcccc tggtaagctt tccaacgctg gcgtcgccgt gccacgtcgg
     3241 gccggtcttg ctcggcggcg aacagagttt tttttgaagc tcagttcggc atcgtgcacg
     3301 acccgccaga cggagccgta actcgtcacc acgccgcgtt caccgagttc cgccaccagt
     3361 gcccgcaacg ttatgtccgg agccgcctcg agccgggcca gaagccaggc ccgttgcggc
     3421 gccagcgaac gcgcctgctg gcggcccatc ggcttcgccg ccgcactccc ggtcgctcgg
     3481 aaacgctgcg accacttcac tacgctcgca atgctgaccc ggaaggtccg tgccgcctcc
     3541 cggcagctcg ccccgcccaa aaccgccgca accacccgct cacgaagatc cagagaatag
     3601 ggtttcgcca tccatgccgg cctccagctc cagccagcat cttgaatcag aaaaccgccg
     3661 agtcgggaat cctaaaagcg attcagacga gatgaatctt gctctaggtt cgcgttgatc
     3721 catgtcaatc gccgcgtctg gacatcgcga tcaaaaaatg aatgctgggg cgtggcgcg
//
LOCUS       DASYNS010000584         3144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_308092, whole genome shotgun sequence.
ACCESSION   DASYNS010000584 DASYNS010000000
VERSION     DASYNS010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308092"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VGP50_17775"
     CDS             <1..117
                     /locus_tag="VGP50_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL14 family ribosomal protein"
                     /protein_id="HEV8029287.1"
                     /translation="VLITKQGEPIGTRIFGPVTRELRARKFMKIVSLAPEVL"
     gene            119..442
                     /gene="rplX"
                     /locus_tag="VGP50_17780"
     CDS             119..442
                     /gene="rplX"
                     /locus_tag="VGP50_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014414498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV8029288.1"
                     /translation="MAAKFKIKKGDKVVVITGRDKGKSGEVLRVVREEDRVLVQGVNM
                     MKRHTRPAAGQPGGIIEKEATIHISNVAHIDPKSNEPTRVGYKILEDGRKVRFAKRSG
                     EVLDK"
     gene            453..995
                     /gene="rplE"
                     /locus_tag="VGP50_17785"
     CDS             453..995
                     /gene="rplE"
                     /locus_tag="VGP50_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV8029289.1"
                     /translation="MQTRLQEHYEKVVKPALVKEFGYANAMQAPKLEKIVINMGVGEG
                     TQDGKKVDAAANDLAIISGQRAVITRAKTSIATFKLRENLPIGCKVTLRRQRMYEFLD
                     RLINIALPRVRDFRGVSAKSFDGRGNYALGLKEQLVFPEVNYDQVDAVRGMDVIIVTT
                     AKTDAEAKALLRGFQMPFVN"
     gene            1018..1323
                     /gene="rpsN"
                     /locus_tag="VGP50_17790"
     CDS             1018..1323
                     /gene="rpsN"
                     /locus_tag="VGP50_17790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV8029290.1"
                     /translation="MAKISSVNKNKRRAQLTKRMAPKRARLKAIADDRSLPPEDRFAA
                     RLKLAEMPRNSSATRIRNRCELTGRPRGYYRKFKLSRIALRELASAGQIPGMVKSSW"
     gene            1337..1735
                     /gene="rpsH"
                     /locus_tag="VGP50_17795"
     CDS             1337..1735
                     /gene="rpsH"
                     /locus_tag="VGP50_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV8029291.1"
                     /translation="MSMTDPLGDMLTRIRNGQRAHKPSIQSPASKIRTNVLEVLQREG
                     YIRSYSREEVRPGIAELKIELKYVDGEPVIREITRVSKPGRRVYSKIADLPRIYNGLG
                     IAILSTPRGVMSDNEARAANVGGEVLCRVF"
     gene            1745..2278
                     /gene="rplF"
                     /locus_tag="VGP50_17800"
     CDS             1745..2278
                     /gene="rplF"
                     /locus_tag="VGP50_17800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003622732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV8029292.1"
                     /translation="MSRIGKNPVTIPQGVTIEVAGQILTAKGKLGTLKLPMSAEVTAA
                     IEDGKVVVTPKGDTKRARVHWGTTRAHINNMVAGVSKGFSKNLEIEGVGYRAAIQGKN
                     LVLQLGYSHDVTFPIPGDVKITCEKPTAITVSGADRQRVGQIAAVIRAFRKPEPYKGK
                     GIKYANEHIRRKEGKKK"
     gene            2290..2655
                     /gene="rplR"
                     /locus_tag="VGP50_17805"
     CDS             2290..2655
                     /gene="rplR"
                     /locus_tag="VGP50_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV8029293.1"
                     /translation="MASKSKDLFTRRRMRVRARIAKHSRGRLRLSVFRSSKHIYAQVI
                     DDTAGRTIAAASSLEKDLRTSLKNGADKAAAAAVGKLVAERAKAAGVSTVVFDRGGYL
                     FHGRVKALADAAREGGLQF"
     gene            2668..>3144
                     /locus_tag="VGP50_17810"
     CDS             2668..>3144
                     /locus_tag="VGP50_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029294.1"
                     /translation="MARTPGGGGGARPGGGPGARPGGRSGGGGRSGGGERSGRGRGEG
                     DRSRGPGGERERDEGDLVEKLVGINRVAKVVKGGRRFGFAAIVIVGDNKGRVGYGAGK
                     AREVPEAIRKATEQARRSMIRVPLREGRTLHHDIIGRYGASEVIVRAAKPGTGIIAG"
BASE COUNT          612 a         1026 c         1076 g          430 t
ORIGIN      
        1 gtgctcatca ccaagcaggg cgagccgatc ggcacgcgca tcttcgggcc ggtgactcgc
       61 gagctgcgcg cccgcaaatt catgaagatc gtctcgctcg cgcccgaggt gctctgacat
      121 ggccgcgaag ttcaagatca aaaaaggcga caaagtggtc gtcatcaccg gccgcgacaa
      181 gggcaagagc ggcgaggtgc tgcgcgtggt ccgcgaggag gatcgcgtgc tcgtccaggg
      241 cgtcaacatg atgaagcgcc acacgcggcc cgccgccggc cagccgggcg gcatcatcga
      301 gaaggaagcg acgatccata tctccaacgt cgcccacatc gatcccaagt cgaacgagcc
      361 gacgcgcgtc ggctacaaga ttctcgagga cggccgcaag gtgcgcttcg cgaagcgctc
      421 cggcgaagtc ctggacaagt gagagacagg ccatgcagac gcgtcttcaa gagcattacg
      481 agaaagtggt gaagcccgct ttggtgaagg agttcggcta cgcgaacgcc atgcaggcgc
      541 cgaagctcga gaagatcgtc atcaacatgg gcgtgggcga aggcacgcag gacggcaaga
      601 aggtcgacgc cgccgccaac gatctcgcga tcatttccgg ccagagggcg gtgatcaccc
      661 gcgccaagac atcgatcgcg acgttcaagc tgcgtgagaa cctgccgatc ggctgcaagg
      721 tgacgctccg gcgtcagcgc atgtacgagt tcctcgaccg gctcatcaac atcgcgctgc
      781 cgcgcgtgcg cgacttccgc ggcgtgtcgg ccaagagctt cgacggccgc ggcaactacg
      841 cgctcggcct caaggagcag ctggtgttcc ccgaggtcaa ctacgaccag gtcgatgccg
      901 tgcgcggcat ggacgtcatc atcgtcacga cggccaagac cgatgcggaa gcgaaggcgc
      961 tgctgcgcgg tttccagatg ccgttcgtca actgagaggc caacgaggtc aagtcccatg
     1021 gcgaagatca gttcggtaaa caagaacaag cggcgggcgc agctgacaaa gcgcatggcg
     1081 ccgaaacgcg cgcgcctcaa ggcgatcgcc gacgaccgct cgctgccgcc cgaggaccgc
     1141 ttcgcggcgc gcctcaagct cgcggagatg ccgcgcaact cctcggcgac gcgcatccgc
     1201 aaccgctgcg agctcaccgg acgcccgcgc ggctactacc gcaaattcaa gctctcgcgc
     1261 attgcgctgc gcgagctcgc ctcggccggc cagatccccg gcatggtcaa gtcgagctgg
     1321 taagggagac gcgctgatgt cgatgaccga tccactcggg gacatgctga cccggatccg
     1381 caacgggcag agggcgcaca agccctcgat ccagtcgccg gcctcgaaga ttcggacgaa
     1441 tgtgctggaa gtgctgcagc gcgagggcta tatccgcagc tactcgcgcg aggaagtgcg
     1501 tccgggcatc gccgagctca agatcgagct gaaatacgtc gatggcgagc cggtgatccg
     1561 cgagatcacc cgcgtctcga agcccggccg gcgcgtctat tccaagatcg ccgacctgcc
     1621 gcgcatctat aacgggctcg gcatcgcgat cctgtcgacg ccgcgcggcg tgatgtcgga
     1681 caacgaagcg cgcgccgcca atgtcggcgg cgaggtgctt tgccgcgtgt tctgaggtga
     1741 cgagatgtcg cgtatcggca agaatccggt gacgatcccg cagggcgtca ccatcgaggt
     1801 ggcggggcag atcctgaccg ccaaaggcaa gctcggcacg ctcaagctgc cgatgagcgc
     1861 cgaggtcacg gccgcgatcg aggacggcaa ggtcgtggtg acgcccaagg gcgacaccaa
     1921 gcgggcgcgc gtccattggg gcacgacccg cgcccatatc aacaacatgg tggcgggcgt
     1981 ctccaagggc ttctccaaga acctcgagat cgagggcgtc ggctatcgcg ccgccatcca
     2041 gggcaagaac ctggtgctgc agctgggata cagccacgac gtgaccttcc cgatcccggg
     2101 cgacgtcaag atcacctgcg agaagccgac cgcgatcacg gtgagcggcg ccgaccgcca
     2161 gcgcgtcggg cagatcgctg cggtcatccg cgccttccgc aagcccgagc cctacaaggg
     2221 caagggcatc aaatacgcca acgagcatat ccgccgcaag gaaggcaaaa agaagtaggg
     2281 accgaagcga tggccagcaa atcaaaggac ctgttcacgc ggcgccggat gcgggtgcgc
     2341 gcgcgcatcg ccaagcacag ccgcggccgg ctcaggctct cagtgttccg ctcctcgaag
     2401 catatctatg cgcaggtgat cgacgatacc gcggggcgca cgatcgccgc cgcctcgagt
     2461 ctcgagaagg acttgcggac gagcctcaag aacggcgccg acaaggccgc ggccgcggct
     2521 gtcggcaagc tcgttgccga gcgcgccaag gccgccggcg tcagcaccgt ggtgtttgac
     2581 cgcggcggat atctcttcca tggccgggtc aaggccctgg ccgatgccgc ccgcgagggc
     2641 gggcttcagt tctaggaaag gcgtttcatg gcacgcacac caggcggcgg cggcggcgcg
     2701 agacccggcg gtggccccgg ggcgagaccc ggcggacgct cgggcggcgg cggacggtcg
     2761 ggcggtggtg aacgctccgg ccgcggccgc ggcgagggcg accgctcacg cggcccgggc
     2821 ggtgagcgcg agcgcgacga gggcgatctc gtcgagaagc tcgtcggcat caaccgcgtc
     2881 gccaaggtgg tgaagggcgg gcgtcgcttt ggttttgccg cgatcgtcat cgtcggcgac
     2941 aacaaggggc gggtcggcta tggcgccggc aaggcgcgcg aggtgccgga ggccatccgc
     3001 aaagcgaccg agcaggcgcg ccgctccatg atccgcgtgc cgctgcgcga gggccgcacc
     3061 ttgcaccacg acatcatcgg ccgctatggc gccagcgagg tcatcgtgcg cgcggccaag
     3121 ccgggcacgg gcatcatcgc cggc
//
LOCUS       DASYNS010000585         5184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2022374, whole genome shotgun sequence.
ACCESSION   DASYNS010000585 DASYNS010000000
VERSION     DASYNS010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022374"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1459)
                     /locus_tag="VGP50_17815"
     CDS             complement(173..1459)
                     /locus_tag="VGP50_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV8029295.1"
                     /translation="MTAELSLARTRSPRFHPASLDALNFLLADVRGALGPYLNVFLVT
                     QQQWSQSSVGLVTTVSGILGLVVQTPIGAAIDATRAKRLLVVAALAVLAAGAAAIYAL
                     PLFWPVLIANTAMAMVGDVFGPAVAALTLGLYGRTGLARRLGRNSAFDHAGNVAIAAA
                     AGAVGWLFAQRAVFLLVPVFAVFAALAVLSIPAGAIDHERARGGEHEPGSSVDAPAGL
                     GILFTCRPLLIYAACAFCFHFANAPLLPLVGQKLAIAHQSLATPMMSSCIIAAQLIML
                     PIALAVGRSADRIGRKPILLIGFAVLPIRALLYTASDNAAWLIGVQLLDGVGAGIFGA
                     ITPLVIADLMRGTGRYNLAQGAVATAQGIGASLSGLAAGVTVDRLGYGAAFTGAAVVA
                     AIALAALALAMPETRQRGLTTSDGRSGSQVSGIGTS"
     gene            1883..3154
                     /locus_tag="VGP50_17820"
     CDS             1883..3154
                     /locus_tag="VGP50_17820"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEV8029296.1"
                     /translation="MKLRRTSLSALMLALSAGVWLTAGSAGAQQASPGLVNPNATTNS
                     ALKDAPQTPAAQKLAPVASPPIAAAADKLPLAQLHAPKDFKIEVYASGLTNARSLRIG
                     DKGTVFVGSRLTDKVYAITSKDGKRAVKTLLTGLYRPNGIALHDGTLYIAELSQISKV
                     DDVENHLDDAPKPQVIYSDLPKDEPHGWKFLAVGPDNRLYFNIGAPCNICMPPETNAQ
                     LRSINLDGSDPQVIARGIRQVVGMDWNPQSKKLYFTENQRDWLSEDIPQDKLNRLDHP
                     GQDNFGFPYCHEGDILDPEFGWGHSCDEFTKPIALLGPHSAPLGMRFYTGHMFPAEYR
                     NQIFIARHGSWNRTHKIGGDIVVATLDKNGNVKSVKPFITGFIQDNKYVGRPADLEVM
                     KDGSLLISDDYNGAVYRVTYAGAQHQLSETR"
     gene            3185..3751
                     /locus_tag="VGP50_17825"
     CDS             3185..3751
                     /locus_tag="VGP50_17825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029297.1"
                     /translation="MRLSLAALGLLAAAALPAAAADLAEQLAPCFACHGDKGQSQTAE
                     VPSLGGQNAPYTLIQLYLFREKQRRFEIMNEAAKGLSDDDLRNVSDAIAALPPPMPME
                     DADPARLERGRALLARYHCNVCHRGDLSGQESVPRIAAQREDYVAKTLRDYKSRARIG
                     YDATMAEALEPVADDEIADVAYAIARQP"
     gene            3788..5077
                     /locus_tag="VGP50_17830"
     CDS             3788..5077
                     /locus_tag="VGP50_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019016429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV8029298.1"
                     /translation="MTELFRLGAREAARRIAAGTLTAQALVEACLERIAAREAAIGAW
                     AWLDPAQALAEARARDQSPVRGPLHGVPIGVKDNMDTADMPTAYGSRCYRGFRPAADA
                     AGVALARAAGAVVLGKTESTEFAAMSPARTRHPDNPAHTPGGSSSGSAAGVADGMMPL
                     AFGSQTAGSIIRPASFCGVVGFKPSFGLVAIAGTKALAPSLDTVGGFARSVDDIAWFI
                     AALTGRPDLAVPEAARRPRLGLFRTQPWDQAQPATVAALDAARQRLGRAGADVRERAA
                     YPAFDGLVPAAKTIMGHEAARSLAWERLNRSSEIMPLTAGLLDEGLAITAQAYDEALR
                     FAAASRGEIPAFFADFDALLVPAAPGEAPPIATTGDPVFNRAWTLLHLPCIALPAGRG
                     PTGLPVGIQLIGRPGEDARLLAVARFAEAALSEASPP"
BASE COUNT          880 a         1813 c         1759 g          732 t
ORIGIN      
        1 tggcgcttcg cgccaagctc aggatgagga tttttcctaa tgccatcaac aaactgccct
       61 catcctgagc gcgggcgaag cgagcagtcc aaggacgaac gatcgacctg cagcccggcg
      121 ctctgcgtgc gtgacgataa attcacaagc tctttcgcgg gaacgaacga tattaggaag
      181 taccgattcc acttacttga gatccgctcc ggccatccga cgtggtgagc ccgcgctgcc
      241 gcgtttcggg catggcgagc gccagtgcgg cgagcgcgat ggcggcgacg acggccgcgc
      301 cggtgaaggc cgcgccatag ccgagacggt cgacggtcac gcccgcggcg agcccgctca
      361 gcgatgcgcc aatgccctgg gccgtggcga ccgcgccttg cgcgaggttg tagcggccgg
      421 tgccgcgcat cagatcggcg atgaccaacg gcgtgatggc gccgaagatg ccggcgccga
      481 cgccgtcgag cagctggacg ccgatcagcc acgccgcgtt gtcggacgcg gtgtagagca
      541 aggcgcggat cggcaggacg gcaaagccga tgagcaggat gggcttgcgt ccgatccggt
      601 cggcggagcg gccgacggcg agagcgatcg gcagcatgat gagctgcgcc gcgatgatgc
      661 aggacgacat catcggcgtc gccagcgact gatgcgcgat ggcaagcttc tggccgacga
      721 gcggcagcag cggcgcattg gcgaagtgga agcagaaggc gcaagcggca tagatcagca
      781 agggacggca ggtgaagagg atcccgaggc cggcgggcgc atcgacgctg gacccgggct
      841 catgctcccc gccgcgcgcg cgttcatggt cgatggcgcc ggccgggatc gacagcaccg
      901 caagcgcggc aaagacggcg aacaccggca ccagcaggaa gacagcccgc tgcgcgaaga
      961 gccagccgac cgcgccggcg gccgccgcga tcgccacgtt gccggcgtga tcgaaggccg
     1021 agttgcggcc gaggcgccgg gcgagaccgg tcctcccata gaggccgagc gtgagcgccg
     1081 cgaccgccgg tccgaagaca tcgccgacca tggccatcgc cgtgttggcg atcagcaccg
     1141 gccagaagag cggcagggca tagatggccg cggctcccgc cgccagcacg gcgagcgccg
     1201 ccacgacgag caggcgcttg gcgcgcgtcg catcgatcgc cgcgccgatc ggcgtctgca
     1261 cgacgaggcc gagaatgccg ctcaccgtcg tgaccaggcc gaccgacgac tggctccatt
     1321 gctgctgcgt cacgagaaag acgttgagat aagggccgag cgcgccccgc acatccgcca
     1381 gaaggaagtt gagcgcatcg agcgacgccg gatggaagcg cgggctgcgg gttcgcgcga
     1441 gcgacagctc tgccgtcatg ggtgcgctct ccggcatcta gcgcagatct cgagcaagta
     1501 gaatcgacac ttcccaacat cgtcattccc gcgaaagcgg gaacccaggg cgagcgacgt
     1561 gccggtggcc cctgggtccc cgctttcgcg gggatgacga cgaaggaagg gttccatcta
     1621 aaccggatgc gcttgcgatg cgccggcggc gaggatcagc ggatcgatcc acaggcaatt
     1681 catcactgct ctccccaagc tttgcgtcgt catcgccatg ggcaaacgtc gctcgacccg
     1741 tgccggccgc atccggtcgc tccggctgga tccatgcggc gcgcagagcg gggccgcgcc
     1801 gaaagcaatg gagcgcttcc ccggactggt gtaacgtccg gccaagccga tcaaccggca
     1861 ttgaggaggg gagagaattg ccatgaagct tcgtcggaca agcctgagcg cgctcatgct
     1921 ggcactgtcg gccggtgtct ggctcaccgc cgggagtgcc ggcgcacaac aggcgtcgcc
     1981 gggattggtg aaccccaatg cgaccaccaa ctcggcgctg aaggatgcgc cgcagacccc
     2041 ggcggcgcag aaactggcgc ctgtggcgtc gccgccgatc gcggcggccg ccgataagct
     2101 gccgctggcg cagcttcacg cgcccaagga cttcaagatc gaggtctatg ccagcggcct
     2161 gaccaatgcg cgctcgctca ggatcggcga caaaggcacg gtgttcgtcg gctcgcgcct
     2221 caccgacaag gtctacgcca tcacgagcaa agatggaaag cgcgcggtga agacgctgct
     2281 gaccgggctc taccggccca acggcatcgc gctccacgac ggtacgctct atatcgccga
     2341 gctctcgcag atctccaagg tcgacgacgt cgagaaccat ctcgacgatg cgccgaagcc
     2401 gcaggtcatt tacagcgacc tgccgaagga cgagccccat ggctggaagt tcctggccgt
     2461 cgggcccgac aaccggctct atttcaacat cggcgcgccc tgcaacatct gcatgccgcc
     2521 cgagaccaat gcgcagctcc gcagcatcaa cctcgacggc agcgaccccc aggtgatcgc
     2581 gcgcggcatc cgccaggtcg tcggcatgga ctggaacccg cagagcaaga agctctactt
     2641 caccgagaac cagcgcgact ggctgtcgga ggacatcccg caggacaagc tcaaccgcct
     2701 cgaccatccg ggccaggaca atttcggctt cccctattgc catgagggcg acatccttga
     2761 tccggagttc ggctggggcc atagctgcga cgagttcacc aagccgatcg cgctgctcgg
     2821 cccgcattcg gcgccgctcg gcatgcgctt ctataccggc cacatgttcc cggccgagta
     2881 tcgcaaccag atcttcatcg cgcgccacgg ctcgtggaac cgcacgcaca aaatcggcgg
     2941 cgacattgtc gtggcgacgc tcgacaagaa cggcaacgtc aaatcggtga agcccttcat
     3001 taccggcttc atccaggaca acaaatacgt cgggcgcccg gccgatctcg aagtgatgaa
     3061 ggacggctcg ctcctgatct cggacgacta taacggagcg gtctaccgcg tcacctacgc
     3121 gggcgctcag catcaactct ccgagacgcg ctgaccgggg gcgacgcagc tcgtctcgcc
     3181 gcgcatgcgc ctttccctcg cggcgctcgg tctgctcgcg gcggcggcgc ttccggctgc
     3241 ggcggcggac ctcgcggagc agctcgctcc ttgtttcgcc tgccacggcg acaagggtca
     3301 gtcgcagacg gcggaagtgc cctcgctcgg cgggcagaac gcgccctaca cgctcattca
     3361 gctctatctc ttccgcgaga agcagcgccg cttcgagatc atgaacgagg ccgccaaagg
     3421 cttgagcgac gacgacctcc gcaacgtctc cgacgcgatc gccgcgctgc cgccgcccat
     3481 gcccatggag gacgccgatc ccgcgcgcct cgagcggggc cgcgcgctcc tggcgcgcta
     3541 tcactgcaac gtctgtcacc gtggcgatct ctcggggcag gaaagcgtgc cgcggattgc
     3601 cgcgcagcgc gaggactacg tcgccaagac cttgcgcgac tacaagagcc gggcgcggat
     3661 cggctatgac gcgaccatgg ccgaggcgct cgagcccgtc gccgacgacg agatcgccga
     3721 cgtcgcctat gccatcgccc gccagccctg agattgtgct atcggccttc gcgaggggag
     3781 ggacgccatg accgaactct tccgtttggg cgcgcgcgag gcggcgcggc ggatcgcggc
     3841 gggcacgctg accgcgcagg cgctggtcga ggcgtgcctc gagcgcattg ccgcgcgcga
     3901 ggccgcaatc ggcgcctggg cctggctcga tcccgcccag gcgctcgccg aggcgcgggc
     3961 gcgcgatcag tcgccggtgc gcgggccgct ccacggcgtc ccgatcggcg tcaaagacaa
     4021 catggacacg gccgatatgc cgacggccta tggctcgcgc tgctatcgcg gattccgtcc
     4081 ggcggccgat gcggcgggcg tcgccttggc gcgggcggcg ggcgcggtgg tgctgggcaa
     4141 gaccgagagc accgaattcg cggcgatgag cccggccagg acgcgccatc ccgacaatcc
     4201 cgcgcacaca ccgggcggct cgtcgagcgg ctcggccgcg ggcgtcgccg acggcatgat
     4261 gccgctcgcc ttcggctcgc agacggcggg ctcgatcatc cgcccggcgt cgttttgcgg
     4321 cgtcgtcggc ttcaagccga gcttcggcct tgtcgccatc gccggcacca aggcgctggc
     4381 gcccagcctc gacacagtcg gcggcttcgc gcgaagcgtc gacgacatcg cctggttcat
     4441 cgccgctctg accggccgcc ccgatctcgc cgtgccggag gcggcgcgcc ggccgcgcct
     4501 cggcctcttc cgcacgcagc cctgggatca ggcgcagccg gcgacggttg ccgcgctcga
     4561 cgcggcgcgc cagcgtctcg gtcgggccgg cgcggatgtg cgcgagcgcg cggcctatcc
     4621 cgcgttcgac gggctcgtgc cggcggcgaa gacgatcatg ggccatgagg cggcgcgcag
     4681 cctcgcctgg gaacggctca accgcagcag cgagatcatg ccgctcaccg cgggtctgct
     4741 ggacgagggg ctcgccatca ccgcccaagc ctatgacgag gccctgcgct ttgccgcggc
     4801 ttcgcgcggc gagatccccg cgttcttcgc cgatttcgac gcgctcctcg taccggcggc
     4861 gccgggcgaa gcgccgccca tcgcaaccac cggcgacccc gtgttcaacc gcgcgtggac
     4921 gctgctccat ctgccctgca tcgcgctccc ggccgggcgc ggtccgacgg ggctgccggt
     4981 cgggatccag ctgatcggcc gcccgggcga ggatgcgcgg ctcctcgccg tcgcgcggtt
     5041 tgccgaggcg gcgctcagcg aagcgtcacc gccctagacg ccctcactca gctttttgcg
     5101 agccgcgaca gcagctccgc cgcgaattgc gagagcgtgt cgtcgcgcgc gcccatcacg
     5161 acgatgcggt cgcccgggcg ggca
//
LOCUS       DASYNS010000586         3732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2961426, whole genome shotgun sequence.
ACCESSION   DASYNS010000586 DASYNS010000000
VERSION     DASYNS010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2961426"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VGP50_17835"
     CDS             <1..361
                     /locus_tag="VGP50_17835"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEV8029299.1"
                     /translation="NYARIVCMRHDTTQILPLSAGAASAVKSVSTLVNRNVTVGGHRT
                     SIRLEPAMWEALHQICRREGKALNELVTEIHRHRAESSLTAAIRVYLLRYFSAAATDE
                     GHRLAGHGLPIGSQLPV"
     gene            complement(346..921)
                     /locus_tag="VGP50_17840"
     CDS             complement(346..921)
                     /locus_tag="VGP50_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008943980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HEV8029300.1"
                     /translation="MPLSPPVAREHIHTRIVECRGFRREDGLWDIEGHITDVKTYAFG
                     NHFRGEIPAGTPVHDMWLRLTVDDRLTIHEVEAVTEASPYEACPNVAPNFARLKGLRI
                     RPGFQAEVRSRLGGTEGCTHLVELLVPVATTAYQTVFPYRERQRREQEKAEGKPRERP
                     SRRPPLLDTCHAFASDGELVKRYWPDFYTGS"
     gene            complement(957..2096)
                     /gene="aroB"
                     /locus_tag="VGP50_17845"
     CDS             complement(957..2096)
                     /gene="aroB"
                     /locus_tag="VGP50_17845"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV8029301.1"
                     /translation="MSAPHRLRVELGARAYEILVGRHLLEQSGVLLGRVLRQQRVIVV
                     TDETVAALHLARFIAGLDAVGIVHEEILLPPGEGTKDFPHFAKLCLDVLKVGIERSTT
                     LVALGGGVVGDLAGFAAATLLRGIDYVQVPTTLLAQVDSSVGGKTAIDTPHGKNLVGA
                     FHQPVMVLADVDVLATLPRRQLLSGYAEVVKYGLIRDRVFYEWLETNGAALLTGDAAV
                     REAAVARSCSLKAAIVAGDERERGERALLNFGHTFGHALEAATGFGDTLLHGEAVALG
                     MRLAFDLSARLGFCSTDTAARIRRHFAAIGLPTDLAQTLGGRFSPKQVLEPMQKDKKV
                     KDGRLTLILVRGIGEAFIVQDVAPALLEEFLAEAMAPGEISRTPP"
     gene            complement(2093..2659)
                     /locus_tag="VGP50_17850"
     CDS             complement(2093..2659)
                     /locus_tag="VGP50_17850"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV8029302.1"
                     /translation="MTATVPLRVPKTIVLVGLMGAGKTSIGRRLAQQLRLPFVDADAE
                     IEAAAGTSIEEIFQRHGEAAFRDGERRVIARLLEERPVQVLATGGGAFMDPETRALIR
                     GRCLSIWLRASLPLMLARVARRNNRPLLKSGDPGPILETLIAERYPIYAEADVTVDSV
                     DGPPELTLERVMTALGDHLAAAERQAAP"
     gene            2825..2965
                     /locus_tag="VGP50_17855"
     CDS             2825..2965
                     /locus_tag="VGP50_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029303.1"
                     /translation="MAKLVLIALGVLVVIVIGGAGVLMVWDIPAPSERVERVIPDAKL
                     PK"
     gene            2946..>3732
                     /locus_tag="VGP50_17860"
     CDS             2946..>3732
                     /locus_tag="VGP50_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029304.1"
                     /translation="MRNCRNEAWRRLSGVAALAALVQLASPAACAEVGPPVPLNAAPP
                     PREAAPQPAATPAEAPAAAGETPAPDAGAGAPAAEAPASAAPPTDESIVATPLAPPDP
                     SWAAALGDGDHPLPATLWQGTPRPLVATLLPQLGPTASPAWQNLARRLLLSGGASPQG
                     EDAPDHPGLPALRVDRLKALGEIAGALAVLDALPSTMRSSDDLDRDHIDLSFAMNDSA
                     GGCRDVKESIARRQGVWWDRALIACQTLAGDQAKASLGVALLRE"
BASE COUNT          604 a         1285 c         1268 g          575 t
ORIGIN      
        1 caattacgct agaattgtat gcatgcgaca cgatactact caaatcctgc ctctgtccgc
       61 gggcgcggcc agcgcagtca aatcggtctc gaccctcgtc aatcgcaatg tgacggtcgg
      121 cggccaccgg accagcatca gactcgaacc ggcgatgtgg gaggcgcttc accagatctg
      181 ccgacgggag ggtaaggccc tcaacgagct ggtcacggag atccaccgtc atcgggcgga
      241 atcgagcctg actgcggcga tccgcgtcta tttgctgcgc tacttctctg ctgcggcgac
      301 cgatgaaggc catcgtcttg ccggccacgg cctgcccatc ggcagtcagc tgccggtata
      361 gaagtccggc cagtagcgct tgacgagttc gccatcgctg gcgaaggcgt ggcaggtgtc
      421 gaggagcgga ggccgccggc tcggcctttc ccggggcttg ccttccgctt tctcctgttc
      481 ccggcgctgg cgctcgcgat agggaaagac cgtctggtaa gcagtggtcg ccaccggcac
      541 cagcagctcg acgagatgcg tgcatccctc ggtgccgccc agccggctgc gcacctccgc
      601 ctggaaaccc ggcctgatgc ggagcccctt gaggcgcgcg aaattcggcg caacgttcgg
      661 gcaggcctca taaggactcg cttccgtcac ggcttcgacc tcgtggatcg tcagccgatc
      721 gtccaccgtc aacctcagcc acatgtcatg cacgggtgtg ccggccggaa tctcaccgcg
      781 gaaatggttg ccgaaggcgt aagtcttgac gtccgtgatg tggccttcga tgtcccacaa
      841 cccatcctcg cggcgaaagc cgcggcattc gacgatgcgc gtgtgaatat gctcgcgtgc
      901 gacaggcgga gagagcggca tggctcgatg tcccggggct ggagggcgga tcggcttcag
      961 ggcggcgtgc gggagatctc gcccggcgcc atggcctcgg cgaggaactc ttcgaggagc
     1021 gcgggcgcca cgtcctgaac gatgaaggcc tcgccgatac cgcggacgag gatcagcgtc
     1081 aggcgcccgt ccttgacttt cttgtccttc tgcatcggct cgagcacctg cttcggcgag
     1141 aatcggccgc cgagtgtctg ggcaaggtcg gtgggcaggc ctatcgcggc aaaatgccgg
     1201 cgtatccgcg ccgccgtatc cgtcgaacag aagccgagcc gggccgagag atcgaaagca
     1261 agccgcatgc cgagcgccac cgcctcgcca tgcagcagcg tatcgccgaa gcccgtcgcc
     1321 gcttcgagcg catggccgaa ggtgtggccg aaattgagca gcgcgcgctc gccgcgctcg
     1381 cgctcgtccc cggcgacgat cgcggccttc aagctgcagc tgcgcgccac ggccgcctcg
     1441 cgcaccgccg cgtcgcccgt cagcagcgcc gccccgttgg tctcgagcca ctcgtaaaag
     1501 acgcgatcgc gaatcaagcc gtatttcacg acctcggcat agccggagag gagctggcgg
     1561 cgagggagcg tggcgaggac gtcgacatcg gccagcacca tcaccggctg gtgaaatgcg
     1621 ccgacgaggt tcttgccgtg cggcgtatcg atcgccgtct tcccgccgac cgagctgtcg
     1681 acctgcgcca gcagcgtcgt cggcacttgc acatagtcga tgccgcgcag cagcgtcgcc
     1741 gcggcgaagc cggcgagatc gccgaccacg ccgccgccca gcgccacgag tgtcgtgctg
     1801 cgctcgatgc cgaccttcag aacatcgaga cagagcttgg cgaaatgcgg gaagtccttg
     1861 gtcccctcgc cgggaggcag caggatttcc tcgtgcacga tcccgacggc atcgaggccc
     1921 gcgatgaagc gcgccaagtg gagcgccgcc acggtctcgt cggtgacgac gatgacgcgc
     1981 tgctggcgaa gcacccggcc gagcagaacg cccgactgct ccagcaggtg gcggccgacg
     2041 agtatctcgt aggcgcgggc gccaagctcg acgcggaggc gatgcggcgc gctcacggtg
     2101 cggcctgccg ctctgccgcg gcgagatggt cgccaagcgc ggtcatcacc cgctcgagcg
     2161 tcagttccgg cggtccgtcg acgctgtcga cggtgacgtc ggcttcggca taaatcggat
     2221 agcgctcggc gatgagcgtc tcgaggatcg ggccggggtc gccgctcttg aggagaggcc
     2281 gattgttgcg ccgcgcgacg cgggccagca tgagcggcag cgaggcgcgc agccagatgg
     2341 agaggcagcg tccccggatg agcgcgcgcg tctccgggtc catgaaggct ccgccgcccg
     2401 tggcgaggac ctggaccgga cgctcctcga gcaggcgggc gatgacacga cgctcgccgt
     2461 cgcggaaggc ggcctcgccg tggcgctgga agatttcctc gatgctggtg ccggcggcgg
     2521 cttcgatttc ggcatcggca tcgacgaaag gcaagcgcag ctgttgtgcc aggcgccggc
     2581 cgatgctggt cttgccggct cccatcaagc cgacgagcac gatggtcttc ggcacgcgga
     2641 gcgggaccgt tgcggtcatt ccgctccgcc gccccctccc tgtgccgtcg ttgaaagcct
     2701 catcgcacgc cgatacactg tccaaaataa gccaagaacg caccctagca tatcgcgtcc
     2761 atggatcgtc aaccggccgg ccctctgccg acgccggcag aaattgcggg aacaagcggg
     2821 gaaaatggca aaactcgtgc tcatcgcgct cggcgtgctg gtcgtcattg tcatcggcgg
     2881 cgccggcgtc ctcatggtct gggatatccc cgcgccgtcg gagcgggtcg aacgggtgat
     2941 ccccgatgcg aaactgccga aatgaggcgt ggcgccgcct gagcggcgtc gcggcgctcg
     3001 ccgcgctggt ccagcttgca agcccggcag cgtgcgcgga agtcggcccg cccgtgccgc
     3061 taaacgccgc gccgccgccg cgcgaagcgg cgccgcaacc cgcggcgacg ccggccgagg
     3121 ctcctgctgc tgcgggcgag actcccgctc ctgacgcagg cgccggcgcc cctgccgccg
     3181 aagctcccgc ttccgccgct cctccgaccg acgaatcgat cgtcgcgacg ccgctcgcgc
     3241 cacccgaccc gagctgggcc gcagcgctcg gtgacggcga ccatcccttg ccggcgactt
     3301 tgtggcaggg aacaccgcgg ccgctggtcg cgactctgtt gccgcagctt gggccgacag
     3361 catcgcccgc ctggcaaaac ctcgcgcgcc ggctgctact gagcggtggt gcgtcaccgc
     3421 aaggagagga cgcacccgac caccctggtc tcccggcact gcgcgtcgat cgattgaagg
     3481 cgctgggcga gatcgcggga gcgctggccg tgctcgacgc gctgccctcg acgatgcgca
     3541 gcagcgatga cctcgaccgc gaccacatcg acctgtcctt cgccatgaat gacagcgccg
     3601 gcggttgccg cgacgtgaag gagagcatcg cgcgtcgcca gggcgtgtgg tgggaccggg
     3661 cgctgatcgc ctgccagacg ctcgcgggcg accaggcaaa ggcgtcgctc ggcgtcgcgc
     3721 tgctgcgcga gc
//
LOCUS       DASYNS010000587         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4674675, whole genome shotgun sequence.
ACCESSION   DASYNS010000587 DASYNS010000000
VERSION     DASYNS010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4674675"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="VGP50_17865"
     CDS             complement(<1..137)
                     /locus_tag="VGP50_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEV8029305.1"
                     /translation="MAELTLPANSKVGAGKTFKAPPGAKRVKAFSVYRWDPEAGQNPR
                     L"
     gene            complement(167..>1502)
                     /locus_tag="VGP50_17870"
     CDS             complement(167..>1502)
                     /locus_tag="VGP50_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003629438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV8029306.1"
                     /translation="HTLYQQCLKHNAEFFVEYVALDLIMDEDGACRGVLAWNLEDGTL
                     HRFRGHIVALATGGCGRAYFSTTQAHTCTGDGNAMALRAGLALQDMEFIQFHPTGIYT
                     AGCLITEGSRGEGGYLTNSEGERFMVRYAPSAKDLASRDVVSRAMTLEMREGRGCGPK
                     KDHLLLHLEHLEPKILHERLPGISETARIFAGVDVTREPIPVLPTCHYIMGGIPTNYH
                     GEVINPTGDNAERIVPGLMAIGEAACVSVHGANRLGSNSLLDLVVFGRAAAQRAAELV
                     RPGAPHKPLANGVDAALDRLDRVRNAKGRLKTGEIRLDMQQAMQNDCAVFRTAAVLEQ
                     GARKIEDVTASMRDLGITDRSMIWNTDLVEALELDNLLAQAATVIHAALNRTESRGAH
                     AREDFPHRDDKDWLKHTVTWLKDGKVTIGYRPVHLYTLSNEVQPFPPKERVY"
BASE COUNT          220 a          485 c          532 g          265 t
ORIGIN      
        1 tcgagacgcg ggttctgccc ggcttcggga tcccagcgat agacgctgaa cgccttcacg
       61 cgcttggcgc ccggcggcgc cttgaaggtc ttgccggcgc cgaccttgga gttggcgggc
      121 aacgttaatt cggccatgac gcgacgctcc ccctcgtctt cttatttcag tagacgcgtt
      181 ccttcggcgg gaagggctgc acctcgttcg acagcgtata gagatgcacc gggcggtagc
      241 cgatggtgac cttgccgtcc ttgagccagg tcaccgtgtg cttcagccag tccttgtcgt
      301 cgcgatgcgg gaaatcctcg cgcgcgtgag cgccgcggct ctcggtgcgg ttgagcgcgg
      361 catggatcac ggtggccgcc tgtgcgagca ggttgtccag ctcgagcgct tccaccaggt
      421 cggtgttcca gatcatcgag cggtcggtga tgccgagatc gcgcatcgag gccgtgacgt
      481 cctcgatctt gcgcgcgccc tgctccagca ccgccgccgt gcggaagacg gcgcagtcat
      541 tctgcatggc ctgctgcatg tcgagccgga tctcgccggt cttgagccgc cccttggcgt
      601 tgcgcacgcg gtcgagacgg tcgagcgcgg catcgacgcc gttggcgagc ggcttgtgcg
      661 gcgcgcccgg ccgcaccagc tcggcggcgc gctgcgccgc ggcgcggccg aacacgacga
      721 gatcgaggag cgagttcgag ccgaggcggt tggcgccatg caccgagacg caagcggcct
      781 cgccgatcgc catgaggcca gggacgatgc gctcggcatt gtcccccgtc gggttgatga
      841 cctcgccgtg atagttcgtg ggaatgccgc ccatgatgta gtggcaggtc ggcagcaccg
      901 ggatcggctc gcgcgtcacg tcgacgccgg cgaagatgcg cgccgtctcg gagatgccgg
      961 gcagccgctc atgcaggatc ttgggctcga gatgctcgag atgcagcagc aggtgatctt
     1021 tcttcgggcc gcagccgcgc ccctcgcgca tctcgagcgt catggcgcgg ctcacgacgt
     1081 cgcgcgacgc caggtccttg gccgagggcg catagcgcac catgaagcgc tcgccctcgc
     1141 tgttggtgag atagccgcct tcgccgcgcg agccttcggt gatgaggcag cccgccgtgt
     1201 agatcccggt cggatggaac tggatgaact ccatgtcctg gagtgccagg ccggcgcgca
     1261 gcgccatggc attgccgtcg ccggtgcagg tatgcgcctg ggtcgtcgag aaataggcgc
     1321 ggccgcagcc gccggtcgcg agcgccacga tatggccgcg gaagcggtgg agcgtgccgt
     1381 cctcgagatt ccaggcgagc acgccgcggc aggcgccgtc ctcgtccatg atgaggtcga
     1441 gcgcgacata ctcgacgaag aactcggcgt tgtgcttcag gcactgctga tagagcgtgt
     1501 gg
//
LOCUS       DASYNS010000588         3641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2179870, whole genome shotgun sequence.
ACCESSION   DASYNS010000588 DASYNS010000000
VERSION     DASYNS010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179870"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /gene="fusA"
                     /locus_tag="VGP50_17875"
     CDS             complement(<1..1494)
                     /gene="fusA"
                     /locus_tag="VGP50_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV8029307.1"
                     /translation="MARTTPLERYRNIGIMAHIDAGKTTTTERILYYTGRSYKLGEVH
                     EGTATMDWMEQEQERGITITSAATTCFWRDHRINIIDTPGHVDFTIEVERSLRVLDGA
                     VTVFDSVAGVEPQSETVWRQADKYGVPRICFVNKMDRIGANFFRCVEMIEDRLGAIPL
                     VTQLPIGVESDFVGVVDLVIMKAIKWEEETLGAKFDVVEIPADMAEQAAEYRAKLVEL
                     AVVQDDKVIEKYLAGEEPDAETLRRCIRKGTISYAFVPVVCGSAFKNKGVQPLLDAVV
                     DYLPAPTDIAGIKGTKLGKDEPVVRHASDDEPFAGLAFKIMTDPFVGSLTFVRVYSGV
                     LPSGTAVLNSVKDQRERIGRMLQMHANHREDIKEARAGDIVALAGLKNTTTGDTLCDP
                     SAAVVLERMEFPEPVIEIAVEPKTKGDQEKMGQALARLATEDPSFRVAVDHESGQTII
                     KGMGELHLEIIVDRMKREFKVEANIGAPQVAYRETISRPANVDYTHKK"
     gene            complement(1513..1983)
                     /gene="rpsG"
                     /locus_tag="VGP50_17880"
     CDS             complement(1513..1983)
                     /gene="rpsG"
                     /locus_tag="VGP50_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV8029308.1"
                     /translation="MSRRRAAVKREVLPDAKFGDAVVTKFMNCLMYEGKKSVAERIVY
                     GALERIAKRAGADPLKVFHDALTNVRPAVEVRSRRVGGATYQVPVEVRTDRGQALAIR
                     WLISSSRDRSEHTMEERLSGELQDAANNRGNAVKKREDTHRMADANKAFSHYRW"
     gene            complement(2009..2380)
                     /gene="rpsL"
                     /locus_tag="VGP50_17885"
     CDS             complement(2009..2380)
                     /gene="rpsL"
                     /locus_tag="VGP50_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV8029309.1"
                     /translation="MPTINQLVNKPRRPQVARNKVPALARSPQKRGVCTRVYTITPKK
                     PNSALRKVARVRLTNGFEVTSYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHIIRGTL
                     DTQGVKDRRQRRSKYGAKRPK"
     gene            2768..3139
                     /locus_tag="VGP50_17890"
     CDS             2768..3139
                     /locus_tag="VGP50_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029310.1"
                     /translation="MANKFTLRGYQIEVDYTVGGDPSFPALTFRRGPVSKSFKPAEIR
                     TEAIALGALVSVPLMKAVDTGGETFAFIVPQIDAPQGQTHFTTAAVLKRFSGPDSVPP
                     RPPSWEAHVLEGTAQTVTVPL"
     gene            complement(3186..>3641)
                     /locus_tag="VGP50_17895"
     CDS             complement(3186..>3641)
                     /locus_tag="VGP50_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV8029311.1"
                     /translation="KHIEVIVRQMLQKVEIENPGDTIFLAGEQIDRPEYDAENLKVER
                     EKMRPATAHPVLQGITKASLQTNSFISAASFQETTRVLTEAAVSGRIDVLSGLKENVI
                     VGRLIPAGTGSVMARWRQLAAERDRVLADQDAEKAAKAVPALEADQKIA"
BASE COUNT          568 a         1212 c         1192 g          669 t
ORIGIN      
        1 cttcttgtgc gtatagtcga cattggccgg ccggctgatg gtctcgcgat aggcgacctg
       61 cggcgcgccg atattggcct cgaccttgaa ctcgcgcttc atgcggtcga cgatgatctc
      121 gagatggagc tcgcccatcc ccttgatgat ggtctggccg ctctcatggt cgacggcgac
      181 gcggaaggac gggtcctcgg tcgcgagccg cgccagcgcc tggcccatct tctcctggtc
      241 gcccttggtc ttgggctcga ccgcgatctc gatgacgggc tcgggaaact ccatgcgctc
      301 cagcaccacg gccgcgctcg ggtcgcagag cgtgtcgccg gtcgtggtgt tcttgaggcc
      361 ggccagcgcc acgatgtcgc cggcgcgcgc ctctttgatg tcctcgcggt ggttcgcgtg
      421 catctgcagc atgcggccga tgcgctcgcg ctgatccttg accgagttga gcaccgccgt
      481 gccgctcggc agcacgcccg aatagacgcg cacgaaggtg agcgagccga cgaagggatc
      541 ggtcatgatc ttgaaggcga ggccggcgaa aggctcatcg tccgaggcat ggcgcacgac
      601 gggttcgtcc ttgcccagct tcgtgccctt gatgccggcg atgtcggtgg gcgccggcag
      661 atagtcgacg acggcgtcca gcagcggctg cacgcccttg ttcttgaagg ccgagccgca
      721 caccaccggc acgaaggcgt aggagatggt gcccttgcgg atgcagcgtc gcagcgtctc
      781 ggcgtcgggc tcctcgccgg ccagatactt ctcgatcacc ttgtcgtcct gcacgaccgc
      841 gagctcgacg agcttggcgc ggtactccgc cgcctgctcc gccatgtcgg cgggaatttc
      901 gacgacgtcg aacttggcgc cgagcgtctc ctcctcccac ttgatcgcct tcatgatcac
      961 gagatcgacg acgccgacga agtcgctctc gacgccgatc ggcagttggg tgacgagcgg
     1021 aatcgcgccc aggcggtcct cgatcatctc gacgcagcgg aagaagttgg cgccgatgcg
     1081 gtccatcttg ttgacgaagc agatgcgcgg cacgccgtat ttgtcggcct ggcgccacac
     1141 cgtctccgat tgcggctcga cgcccgccac cgaatcgaac accgtcaccg cgccgtcgag
     1201 gacgcgcaag gaacgctcga cctcgatggt gaagtcgacg tgccccggcg tgtcgatgat
     1261 attgatgcgg tgatcgcgcc agaagcaggt ggtcgcggcc gaggtgatgg tgatgccgcg
     1321 ctcctgctcc tgctccatcc agtccatggt ggccgtgccc tcatggacct cgcccagctt
     1381 gtaggagcgg cccgtgtagt aaaggatgcg ctcggtcgtc gtggtcttgc cggcatcgat
     1441 atgggccatg atgccgatgt tgcgatagcg ctcgaggggg gtcgtgcgtg ccatggaaag
     1501 ctccggaccg tcttaccagc ggtagtgtga gaacgccttg ttggcgtccg ccatacgatg
     1561 ggtgtcttcc cgcttcttga cggcgttgcc gcgattattg gcggcgtctt ggagttcccc
     1621 cgagagcctt tcctccatgg tatgttccga gcgatcgcgc gagctgctga tgagccagcg
     1681 gatggccagc gcctggccgc gatcggtgcg cacttcgacc ggcacctgat aggtggcgcc
     1741 gccgacccgg cgggagcgca cctcgacggc cggccgcaca ttggtgaggg cgtcgtggaa
     1801 gaccttcagc gggtcggcgc ccgcgcgctt ggcgatgcgc tcgagcgcgc catagacgat
     1861 gcgctcggcg accgacttct tgccctcata catcaggcag ttcatgaatt tggtcacgac
     1921 ggcatcgcca aatttggcgt ccggcaggac ctcccgcttg accgcagcgc gacgacgcga
     1981 catgacgttg ctcctggctc tccttaactt acttcggccg cttggcgccg tatttggagc
     2041 gccgctggcg gcggtccttg acgccctggg tatcgagcgt gccacggatg atgtggtagc
     2101 gcacacccgg cagatccttg acgcggccgc cgcgaatcag cacgaccgaa tgctcctgaa
     2161 ggttatgccc ctcgccgggg atgtagctcg tcacctcgaa accgttggtg aggcgcacgc
     2221 gcgcgacttt gcgcaacgcc gaattgggct tcttcggcgt gatggtgtag acgcgcgtgc
     2281 agacaccacg cttctgcggc gagcgcgcca gcgccgggac cttgttgcgc gccacctgcg
     2341 ggcggcgcgg cttgttcacc agctgattga tcgtcggcat gctctaagcg acctcatcaa
     2401 gcgcaatcgc cgcgcgacgt gcttcccgca ccgtcgacac ggctcgaagg gaactccgga
     2461 aacgcgccgt ctttcacggc gccgggccaa aaccgaaatc ggctgcgtct aggacggcct
     2521 gcctaccgcc agccccccat tcagggtgcg cggatactag tgacgcgggc agaaggcgtc
     2581 aagccgtatc ggccatcttg gtgaacgcgc cgccgcgggc gaccagcgaa ccgaagagcg
     2641 ccccggccgg agaaccgggc cggagcgacc tgtgaggcgg ttcatatccg cacgcgtcgc
     2701 gagcggctta ggattgagtg tcggcgaggc ctcaaacgcc ttcgcttccc cgagcatgga
     2761 ggccatgatg gcgaacaagt tcacgcttcg aggctatcag atcgaggtcg actatacggt
     2821 cggcggcgat ccctcttttc ccgcgctgac cttcaggcgc ggcccggtct ccaagagttt
     2881 caagccggcc gagatccgca ccgaggcgat cgcgctgggc gcgctcgtgt ccgtgccctt
     2941 gatgaaggcc gtcgacaccg gcggcgagac cttcgccttc atcgtgccgc agatcgacgc
     3001 gccccaagga cagacgcatt tcaccaccgc cgccgttctc aagcgtttca gcggccccga
     3061 ttccgtgccg ccccgccccc cgagctggga agcgcatgtg ctcgaaggaa ccgcgcaaac
     3121 ggtgaccgta ccgctctgag caagccccct ctcccgcacc gcgggagagg gaagccgccg
     3181 aaagatcagg cgatcttctg gtccgcctcg agggctggaa cagccttcgc ggccttctcg
     3241 gcatcctggt ccgcgagcac gcggtcgcgc tcggcggcga gctggcgcca gcgcgccatc
     3301 acgctgccgg tgccggccgg gatgagccgg ccgacgatga cgttctcctt caagcccgag
     3361 agcacgtcga tgcggcccga caccgccgcc tccgtcagca cccgcgtcgt ctcctggaag
     3421 gaggcggcgg agatgaagga gttggtctgc aggctcgcct tggtgatgcc ctgcagcacc
     3481 ggatgcgccg tcgccggccg catcttctcg cgctcgacct tgaggttctc ggcatcgtat
     3541 tcggggcggt cgatctgctc gccggcgagg aagatggtgt cgcccggatt ctcgatctcc
     3601 accttctgca gcatctggcg gacgatcacc tcgatgtgct t
//
LOCUS       DASYNS010000589         3479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2023237, whole genome shotgun sequence.
ACCESSION   DASYNS010000589 DASYNS010000000
VERSION     DASYNS010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2023237"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..985)
                     /locus_tag="VGP50_17900"
     CDS             complement(179..985)
                     /locus_tag="VGP50_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007005427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEV8029312.1"
                     /translation="MSDGIEPFELFAIRYAHHAKRSAAENFIGGDVHEDGSALDYFVW
                     VARRSDRVFVIDTGFAEKALRPGRQLLRLPSDGVRALGVAPEQVEDVIITHLHYDHAG
                     TLDAFARARFHLQDREMAYATGRCMCHPALRHAFAVDDIAAMVRYVYAGRVAFHDGDE
                     ELVPGLSVHHIGGHTAGIQSVRVLTPRGYVVLASDASHLYANMEEGRPFPIVYNVADM
                     LEGHRRLQRLAASPRHVVPGHDPLVMRRYAPPSRALEGVAVRLDAEPLSR"
     gene            complement(982..2064)
                     /locus_tag="VGP50_17905"
     CDS             complement(982..2064)
                     /locus_tag="VGP50_17905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV8029313.1"
                     /translation="MEFGILFTSHPNTEVEPYPHRAVHERVTQEILRAEALGFDYAWI
                     AEHHFSNQYGIMPDVFVYAAYLAALTKRIKIGTAVVTLPLANPLRVAENAAFVDILSS
                     GRFALGLGSGYRQYEFDGFGVDFATRRDVQEEALPLLIELLLKRRADHRGRHFNVKVA
                     GDYELFPHAVQAPHPPLFLAGATERSIAVAGRMGLGLMLSTWTPFEELARQVGAYRAA
                     LEETPPALRGNPGRGQVDIARWCYVADSDARAKSESEAGLLRHLSHFASGHTSGYLGT
                     VQKDSGGRPRDYDALARDVILHGSAATVVEKIERLRDMTGASSIMLHYPPWYGAERTL
                     ASLGRFADAVMPKLRKPSPAAAKARS"
     gene            2155..3054
                     /locus_tag="VGP50_17910"
     CDS             2155..3054
                     /locus_tag="VGP50_17910"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8029314.1"
                     /translation="MSERRIGFIGVGRMGLPMASRLLAAGHKLVAYDVQGQALGAIAA
                     KGAETATSPAEVASRAEIVLASLPMPDVVREVALGGRGLVEGSRIRTFVDLSTTGPRM
                     AKAVAAELAKREIAAIDAPVSGGVAGAVKGTLAVMVSGPRAEAETLRPMLEAIGRAFY
                     IGAEPGQGQMMKLINNLLSATATAATSEAVVLGVKAGLDPTVMIDVINAGSGRSTASE
                     DKFPRAILPRRFDYGFALGLMAKDVNLCIAEAEALGVPMWIGNAVKQLWLYGLAQGGP
                     EQDFTELIKHLEKWAGVRVGSSR"
     gene            3095..>3479
                     /locus_tag="VGP50_17915"
     CDS             3095..>3479
                     /locus_tag="VGP50_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HEV8029315.1"
                     /translation="MDILVYSGLELLGDGVMKLPFLRALRQRWPEARITWLAGKGETV
                     YAHELAPLASASLDEIIENAGIGSRAAELLYRPLPSRRFDLVIDTQRRVLTTLILRRL
                     RTCCFVSGAARFLLSDRRPARGYQRP"
BASE COUNT          578 a         1179 c         1197 g          525 t
ORIGIN      
        1 atcgtcatcg acccggcggg ggccggccgg actgcgttta ccatggtgcc gcaaatacat
       61 ggggcgacac gcctcgccgc ttgagtaact tctcggaaag catactgatc cgcgcaatcg
      121 gcgccacgcc ttttacttaa aatccgattg aattttcacc aaatcaccgc gacgactttc
      181 atcgcgacaa cggctcggcg tcgaggcgca cggcgacacc ttcgagcgcg cgcgacggcg
      241 gcgcatagcg ccgcatcacc agcggatcgt gtcccggcac gacgtgccgc ggcgacgcgg
      301 cgagacgctg cagccggcgg tgaccctcga gcatgtcggc gacgttgtag acaatgggga
      361 aaggccgccc ctcctccatg ttcgcgtaga ggtggctcgc atcggaggca agtacgacgt
      421 agccgcgcgg cgtcagcacg cgcaccgact gaatgccggc ggtgtgcccg ccgatgtgat
      481 ggacggaaag gccggggacc agttcctcgt cgccgtcatg gaacgcgaca cgcccggcat
      541 agacgtagcg caccatcgcg gcgatgtcgt cgacggcgaa agcatggcgc agcgccggat
      601 ggcacatgca ccgcccggtc gcataggcca tctcgcggtc ctgcagatgg aagcgcgcgc
      661 gggcgaacgc atcgagcgtg cccgcatggt cgtagtggag atgcgtgatg atgacgtcct
      721 cgacctgctc cggcgcaacg ccgagcgcgc gcacgccatc ggacgggagc cgcagcagct
      781 ggcgcccggg ccggagcgct ttctcggcga agcccgtgtc gatgacgaag acgcggtccg
      841 agcgccgcgc gacccagacg aaatagtcga gggccgagcc gtcctcatgc acgtcgccgc
      901 cgatgaaatt ctcggcggcg ctgcgcttcg cgtgatgggc atagcggatg gcgaagagct
      961 cgaagggctc gatcccgtcg ctcatgagcg cgccttcgcc gccgcgggcg agggtttccg
     1021 gagcttcggc atgaccgcgt cggcgaagcg cccgagcgac gccagcgttc gctcggcgcc
     1081 gtaccagggc ggatagtgca gcatgatcga gctcgcgccg gtcatgtcgc gcaggcgctc
     1141 gatcttctcg accaccgtcg ccgccgagcc atgcaggatg acgtcgcgcg cgagcgcatc
     1201 atagtcgcgc ggacgcccgc ccgagtcctt ctgcaccgtg ccgagatatc ccgaggtgtg
     1261 gccgctcgcg aaatggctca gatggcggag gagaccggct tcgctctcgc tcttcgcccg
     1321 ggcgtcgctg tcggcgacat agcaccagcg ggcgatgtcg acctggccgc ggccgggatt
     1381 gccgcgcagc gccggcggcg tctcctcgag cgccgcgcga taggcgccga cctggcgcgc
     1441 cagctcctcg aacggcgtcc aggtcgacag catcagcccg agccccatgc gcccggcgac
     1501 cgcgatcgag cgctcggtcg cgccggcgag gaagagcggc ggatgcggcg cttgcacggc
     1561 gtgcgggaag agctcgtagt cgcccgcgac cttgacgttg aaatgcctgc cgcgatggtc
     1621 ggcgcgccgc ttcagcagca gctcgatcag cagcggcagc gcctcttcct ggacgtcgcg
     1681 gcgcgtcgcg aaatcgacgc cgaagccgtc aaactcgtat tggcgatagc ccgagcccag
     1741 acccaaggcg aagcggccgc tgctcaggat atcgacaaag gccgcgttct cggcgacgcg
     1801 gagcggattg gcgagcggca gcgtcaccac cgccgtgccg atcttgatcc gcttggtgag
     1861 cgccgcgaga taggccgcgt agacgaacac gtccggcatg atgccgtatt gattgctgaa
     1921 atggtgctcg gcgatccagg catagtcgaa gcccagcgcc tcggcgcgca ggatctcctg
     1981 cgtcacccgc tcatggacgg cgcgatgcgg ataaggctcg acctcagtgt tggggtgaga
     2041 cgtgaagaga atgccgaatt ccatgagtcc ctcgctttcg ccttgagccc ggcgttggct
     2101 accatcatca caccgcgctt cggacgcgcc aagagaaacg aagggagaag cggtttgagc
     2161 gaacgcagga tcggcttcat cggcgtcggc cgcatggggc tcccgatggc atcgcggctc
     2221 ctggccgccg gccacaaact ggtcgcctat gacgtgcagg gacaggcgct gggtgccatt
     2281 gcggccaagg gcgccgaaac cgcgacctca cccgcggaag tggcatcgcg cgccgagatc
     2341 gtgctggcga gcctgccgat gccggacgtg gtgcgcgagg tggcgctggg cgggcgcggc
     2401 ctcgtcgagg gatcgcgcat ccggaccttc gtcgaccttt ccaccaccgg cccgcgcatg
     2461 gccaaggcgg tcgcggccga gctggcgaag cgcgagatcg ccgccatcga cgcaccggtg
     2521 agcggcggcg tcgccggcgc ggtcaaaggc acgctcgcgg tgatggtgtc ggggccgcgc
     2581 gccgaagccg agacgctccg gccgatgctc gaggcgatcg gccgcgcctt ctacatcggc
     2641 gcagagccgg gccaggggca gatgatgaag ctcatcaaca acctgctctc ggcgaccgcg
     2701 acggcagcga catcggaagc cgtcgtgctc ggcgtcaagg cggggctcga tcccacagtg
     2761 atgatcgacg tgatcaatgc cgggagcggc cgcagcacgg cgagcgagga caagttcccg
     2821 cgcgccatcc tgccgcgccg cttcgactac ggcttcgcgc tcggcctcat ggcgaaggac
     2881 gtcaacctct gcatcgccga ggccgaggcg ctcggcgtcc ccatgtggat cggcaacgcc
     2941 gtgaagcagc tctggctcta cggcttagcg caaggcggcc cggagcagga cttcaccgag
     3001 ctcatcaagc atctcgagaa atgggccggc gtgagggtgg gatcgagcag atgatcgtct
     3061 ccgcctctac agagaatgag acctttgccg gctgatggat atcctcgtct attccggcct
     3121 cgagcttctg ggcgacggcg tgatgaagct gcccttcctg cgcgcgctgc ggcagcgctg
     3181 gcccgaggcg cgcatcacct ggctcgccgg caagggcgag acggtctacg cccatgaact
     3241 cgcgccgctg gcgagcgcgt cgctcgacga gatcatcgag aatgccggca tcggctcgcg
     3301 cgcggccgag ctcctgtacc ggccgctgcc cagccgccgc ttcgacctcg tcatcgacac
     3361 gcagcgccgc gtgctcacca cgctcatcct gcgccggctc cgcacgtgct gcttcgtctc
     3421 gggcgcggcc cgctttcttc tctctgaccg gcggccggcg cgcggctacc agcgtccgg
//
LOCUS       DASYNS010000590        10679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4675062, whole genome shotgun sequence.
ACCESSION   DASYNS010000590 DASYNS010000000
VERSION     DASYNS010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10679
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4675062"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1958)
                     /gene="carB"
                     /locus_tag="VGP50_17920"
     CDS             complement(<1..1958)
                     /gene="carB"
                     /locus_tag="VGP50_17920"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV8029316.1"
                     /translation="MPKRTDIKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYRI
                     VLVNSNPATIMTDPGLADATYIEPITPAMVERIIAKERPDALLPTMGGQTALNIAMAL
                     ARAGVLKRYGVELIGASEAAIDKAEDRQLFREAMDRIGLTSPKSRLVRSLDEAIAVMG
                     EIGLPAVIRPSFTLAGTGGGIAYNTEEFEEIVRGGLRASPTREVLIEESVLGWKEYEM
                     EVVRDCADNCIIVCSIENIDPMGVHTGDSVTVAPALTLTDKEYQIMRNASIAVLREIG
                     VDTGGSNVQFAVNPADGRLVIIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LKNEITRTTPASFEPTIDYVVTKMPRFTFEKFPGATPLLTTSMKSVGEAMAIGRSFAE
                     SVQKALRSLETGLTGFNEVEIPGAAESDRNAVRAALAMPTPDRLLVIAQAYRHGLTTA
                     EIHAACKYDPWFLEEVRRIVLAEAEIRAKGLPEDAAGLLRLKQQGFADARLAELAGCE
                     EAAVAARRAKLGVHPVYKRIDTCAAEFPSLTPYMYSTYEGDGASPPECEAEPSERRKV
                     VILGGGPNRIGQGIEFDYCCVHAAYSLKEAGFETIMVNCNPETVSTDYDTSDRLYFEP
                     LTAEDVIELVRVEERRGTLLGVVVQLGG"
     gene            complement(1965..3140)
                     /gene="carA"
                     /locus_tag="VGP50_17925"
     CDS             complement(1965..3140)
                     /gene="carA"
                     /locus_tag="VGP50_17925"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009542306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV8029317.1"
                     /translation="MIDATLSHAPPRPPGTTAALALADGSVFWGKGVGAPGLAVGEVC
                     FNTSMTGYQEIITDPSYAGQIITFTFPHIGNVGANPEDIETMTPAARGLVLRVDITLP
                     SNYRAAQHFDAWLKSHNLVGISGVDTRRLTRRIRDLGAPSGALSHAPDGAIDLAELAA
                     KAKAWPGLEGMDLAKEVTCRQTYSWDETRWERGRGYGRLTAPRFHVVAVDYGAKRNIL
                     RMLASHHCRVTVVPATASAADILRHRPDGVFLSNGPGDPAATGDYAVPVIRDLLAAEK
                     PLFGICLGHQLLALALGAKTRKMAIGHRGANHPVKDLATGKVEITSQNHGFCVLPETL
                     PASAAVTHVSLFDGSNEGLRLGDKPVFSVQYHPEASPGPQDSHYLFQRFVEMMERPR"
     gene            3351..3806
                     /locus_tag="VGP50_17930"
     CDS             3351..3806
                     /locus_tag="VGP50_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008889523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEV8029318.1"
                     /translation="MLRQKVAEALKTAMKEKDALVVSTVRMMMAKLKEQDIEARAKGN
                     TDGIGESEIQQMLQGMIKQRRESIVLYDKGGRPELAEKERGEIAIIERFLPTQLSDEA
                     LEAAVKETIAATGAQGMKDMGRTMTALRERYAGQIDGAKASQVVKRLLG"
     gene            3932..5767
                     /gene="dnaG"
                     /locus_tag="VGP50_17935"
     CDS             3932..5767
                     /gene="dnaG"
                     /locus_tag="VGP50_17935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV8029319.1"
                     /translation="MAFPPRFLDELRARVSLAEVVGRRVRLIRRGREYVGLCPFHKEK
                     TPSFSVVEDKGFYHCFGCGVHGDVIGFAMQTESMTFPEAVEALARQAGLDVPTQSPED
                     REKIERQATLQAAMEAACAFFEEQLHAVPGRDARLYLEGRGVDLATLRRFRLGFAPDR
                     RDGLLRALAKRFPEALLIEGGLARRSEEGESFDYFRRRIIFPIGDRQGRIIAFGGRVL
                     GEGQPKYLNSPDTPLFQKGRVLYGWAPARAAAARAPSAIVTEGYMDVIALHRAGFTTA
                     VAPLGTALTEMQLEELWRLAPEPVLCFDGDAAGLRAAGRALERALPLLKPGQSLTFAT
                     LPEGEDPDTLILRHGVGAMTEVLERARPLADVLWELETARPCVTPEQRAALERRLEER
                     ARAIADRSVQEHYRSFFRSRLFDALRRNNTGARRWRPGRPAGPNVGVQLKTPAALEAA
                     PRSEPTPLRRRRQEILLALLLNHPFLLSDVAEELAEIQPGPPDLDKLRHEILRLHALN
                     PDLDAATLRLHLTKSGYDRVVEGVLSSQVLAHAAFARAGADAETVRLGWADTRDQLRQ
                     PHLRTQIAEAERELAEETTEENWVRLHPLLEQKIEDDGATGGGSR"
     gene            5886..7916
                     /gene="rpoD"
                     /locus_tag="VGP50_17940"
     CDS             5886..7916
                     /gene="rpoD"
                     /locus_tag="VGP50_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV8029320.1"
                     /translation="MASKAANAAEAPETREEAAEAPLLNTVEQAIRKMVARGKERGYV
                     TFDEVNAALPTEQVSPEQIEDTLAMLNEQGINVVESEETDEAAATEGGDGENEVRAGN
                     LDDEDIGRTDDPVRMYLREMGSVELLSREGEIAIAKRIEAGREMMIGGLCESPLTMRA
                     LIAWRDALTEGKMLLRDVIDLDATYGSAFAEAVEEVAAEVEAEAEAAATTTTTEADAV
                     EEPVVPVPEAGNGQDPSAVALPPPAPGEEDLDGEDTSISLAAMEAELKPSVLATLDGI
                     AALYKRLQKVQGQRLTTLQEGEELARPQEKRYDKLKSEMVDAMKSVRLNNGRIENLVG
                     QLYALNRRLVAAEGRLLRMAESSGVKRQDFLNHHMGQELAPDWTTRVKRLHGKGWGSL
                     VNKHGTEVKTLRGEVAAISEEAGLPPIEFRRIVSTVQRGEREAGRAKKEMVEANLRLV
                     ISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIAD
                     QARTIRIPVHMIETINKLVRTSRQMLHEIGREPTPEELAEKLAMPLEKVRKVLKIAKE
                     PISLETPIGDEEDSHLGDFIEDKNAILPLDAAIQANLRETTTRVLASLTPREERVLRM
                     RFGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKLRSFLDT"
     gene            complement(7968..8798)
                     /locus_tag="VGP50_17945"
     CDS             complement(7968..8798)
                     /locus_tag="VGP50_17945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029321.1"
                     /translation="MAVDLATGKVTPNLVPSTRGHAALPIPDSTRVLSTSGGANTATI
                     FEGMTGEIVASLAVGKNPDAVAYDPATRTLWVMNPGSGDISVIDPAIAQVVATVAVGG
                     SLELGAADGEGRLYVNVEDQNDVAVIDTRTRQLVTRFPLMGCDRPTGIAYAPDVRLIV
                     SACANGVAKVSAPDGHEMASLAIGPRPDGALYDAQRHLAFVPSGGDGTLAVIRLSPTP
                     EIIERVETAKGARTAALDPATGRIYLPSAQYAPAIGAARPAALPGSFTVLVVAPDRPQ
                     "
     gene            complement(9105..9596)
                     /locus_tag="VGP50_17950"
     CDS             complement(9105..9596)
                     /locus_tag="VGP50_17950"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEV8029322.1"
                     /translation="MTSRIIVERVSVTSSRPFEIVVAALEAVVGRPNMAEFNKEIGSA
                     TSFGAMQQTIQRALGTSGFMEFIRFDLGSILRRESGRQGPKVLRLVIGNPLIMKEMVK
                     HVADAGSYAPVTILIDERPDGVHLSYDKMASFLEGYGNPDALKVARELDSRVEALLTE
                     AAG"
     gene            complement(9608..>10679)
                     /locus_tag="VGP50_17955"
     CDS             complement(9608..>10679)
                     /locus_tag="VGP50_17955"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV8029323.1"
                     /translation="DYAARREEQRALRGSGRYRGIGVAAYTEMCGMAPSRRLAASGFD
                     RGGWESARVNVDSSGKVTLFSGSMSQGHGHATVFAQIAADELQLPVEHIEVVQGDTRQ
                     VQAGHGTFNSRSIPVGGSSVKVCARRIVAKARKIAAAMMEVAEPDVLYERGHFRVAGT
                     DIAPLSFARVARMAYVGHALPNDVEPGLDETLFYDPTGMSAPSGVHMAYVEVDPETGT
                     VEILDYVAVDDAGTIINPLLAAGQIHGGVVQGIGQALYEEVCYDGETGQLMTGSLLDY
                     AVPRADSVPNIRTQFQETPSPTNPLGAKGIGESGAIGAPPTIVHAVLDALAPFAIGHL
                     DMPLTPLKIWTAIQKARAEANS"
BASE COUNT         1861 a         3470 c         3677 g         1671 t
ORIGIN      
        1 tggccgccca gctgtacgac gacgccgagg agcgtgccgc ggcgctcctc gacgcgcacc
       61 agctcgatca cgtcctcggc ggtgagcggc tcgaaataga gccggtccga ggtgtcgtag
      121 tcggtcgaga cggtctcggg attgcaattg accatgatgg tctcgaagcc agcctccttg
      181 aggctgtaag cggcgtggac gcagcaatag tcgaactcga tgccctggcc gatgcggttc
      241 gggccgccgc ccaggatcac caccttgcgg cgctcgctgg gctctgcctc gcattcgggc
      301 gggctggcgc cgtcgccctc ataggtcgaa tacatgtagg gcgtgagcga cgggaactcg
      361 gcggcgcagg tatcgatgcg cttatagacg ggatggaccc cgagcttggc acgccgcgcc
      421 gcgaccgccg cctcttcgca tcccgcaagc tcggcgagcc gcgcatcggc gaagccctgc
      481 tgcttcagcc gcaaaaggcc ggccgcgtcc tcgggcaggc ccttggcgcg gatttcggcc
      541 tcggccaaca cgatgcggcg gacctcctcg aggaaccacg gatcgtattt gcaggcggcg
      601 tggatctcgg ccgtggtgag gccgtggcga taggcctggg cgatcaccaa cagccggtcg
      661 ggcgtcggca tggccagcgc ggcgcggacg gcgttccggt cgctctcggc ggcgcccggg
      721 atctcgacct cgttgaagcc ggtgagcccg gtctcgagcg agcgcagcgc cttctgcacg
      781 gactcggcga aggagcggcc gatcgccatg gcctcgccga ccgatttcat cgacgtcgtg
      841 agcagcggcg tcgcgcccgg gaatttctcg aaggtgaagc gcggcatctt ggtgacgacg
      901 taatcgatcg tcggctcgaa ggatgccggg gtggtccgcg tgatctcgtt cttgagctcg
      961 tcgagcgtgt agcccacggc gagcttcgcc gcgaccttgg cgatggggaa gccggtcgct
     1021 ttcgacgcca gcgccgagga gcgcgagacg cgcgggttca tctcgatgat gacgaggcgg
     1081 ccatccgcgg ggttgacggc gaactgcaca ttggagccgc cggtgtcgac gccgatctcg
     1141 cgcagcaccg caatcgaggc gttgcgcatg atctgatatt ccttgtccgt gagcgtgagc
     1201 gccggcgcca cggtgacgct gtcgccggta tgcacgccca tcgggtcgat attctcgatg
     1261 gagcagacga tgatgcaatt gtcggcgcag tcgcgcacga cctccatctc gtactccttc
     1321 cagcccagca ccgattcctc gatcagcacc tcgcgagtcg gcgaggcgcg caggccgccg
     1381 cgcacgatct cctcgaactc ctcggtgttg taggcaatgc cgccgccggt gccggccagc
     1441 gtgaaggacg ggcggatcac ggccggaagc ccgatctcgc ccatgacggc gatcgcttcg
     1501 tcgagcgagc gcacgaggcg gctcttgggc gaagtgaggc cgatgcggtc catcgcctcg
     1561 cggaagagct gccggtcctc ggccttgtcg atcgccgcct cgctggcgcc aatgagctcg
     1621 acaccgtagc gcttcagcac gccggcgcga gcgagcgcca tcgcgatatt gagcgcggtc
     1681 tgtccgccca tggtggggag gagcgcatcg gggcgctcct tggcgatgat gcgctcgacc
     1741 atcgcgggcg tgatgggctc gatataggtc gcatcggcga gacccgggtc ggtcatgatc
     1801 gtcgccgggt tcgagttcac gaggacgatg cgatagcctt cctcgcgcag cgccttgcag
     1861 gcctgcgcgc cggaataatc gaactcgcag gcctggccga tgacgatcgg cccggcgccg
     1921 atgatgagga tgctcttgat gtcggtgcgc ttaggcatca ggcgtcaccg cgggcgttcc
     1981 atcatctcga cgaagcgctg gaagaggtag tgactgtcct gcggtcccgg gctcgcttcg
     2041 ggatggtatt gcaccgagaa gaccggcttg tccccgaggc gcaggccctc gttgctgccg
     2101 tcgaagagcg agacatgcgt gacggcggcg ctcgcgggca acgtctccgg cagcacgcag
     2161 aagccgtgat tctggctggt gatctcgacc ttgccggtgg cgaggtcctt cacaggatga
     2221 ttggcgccgc gatggccgat cgccatcttg cgcgtcttgg ccccgagcgc cagcgccagc
     2281 agctgatggc cgaggcagat gccgaagagc ggcttctccg ccgcgaggag atcgcggatc
     2341 accggcacgg catagtcacc ggtcgccgcc ggatcgccgg ggccgttgga caggaacacg
     2401 ccgtcgggac ggtggcgcag aatgtcggcg gccgacgccg tcgccggcac cacggtgacg
     2461 cggcagtgat gcgacgccag catgcgcagg atgttgcgct tggcgccgta gtcgacggcg
     2521 acgacgtgaa agcgcggcgc cgtgagccgg ccatagccgc ggccgcgctc ccagcgtgtc
     2581 tcgtcccagg aataggtctg gcggcaggtc acttccttgg cgaggtccat gccttcgagc
     2641 ccgggccagg ccttcgcctt ggcggcgagc tcggcgagat cgatcgcgcc gtccggcgcg
     2701 tgagagagcg ctcccgacgg cgcgccgaga tcgcgaatgc gccgcgtcag ccgccgcgtg
     2761 tcgacgccgg agatgccgac gaggttgtgc gatttgagcc aggcatcgaa gtgttgcgcg
     2821 gcgcgataat tggagggaag cgtgatgtcg acacgcagca cgaggccgcg cgccgccggc
     2881 gtcatcgtct cgatgtcctc gggattggcg ccgacattgc cgatatgcgg gaaggtgaag
     2941 gtgatgatct ggccggcata ggagggatcg gtgatgatct cctgatagcc ggtcatgctg
     3001 gtgttgaagc agacctcgcc gacggccaag cccggcgcgc ccacgccctt tccccaaaaa
     3061 acgctgccat cggcgagggc cagagcggcc gtggtaccgg gcgggcgcgg gggcgcgtgc
     3121 gacagcgtcg cgtcgatcat gcgggggcgt atcctcgaac tctggctggt ggaccgcccg
     3181 cgccaaagcg caccgcggca gtgcctcggc ggcgcaacga actgcgatcg gacaaccgcc
     3241 gccacatagc cgctcgaaag gatggcgtca aggccggcgt gtcgctcgaa ttgctaattg
     3301 cgctggcgcc gggtaacgtc gagcgagcac gcggttcgcg gagaaatctc atgttgcggc
     3361 agaaggtcgc ggaagccctc aagaccgcga tgaaggagaa ggacgcgctc gtcgtctcga
     3421 cggtccggat gatgatggcc aagctcaagg agcaggacat cgaggcgcgc gccaaaggca
     3481 acaccgacgg catcggcgaa agcgagatcc agcagatgct gcagggcatg atcaagcagc
     3541 gccgcgagtc gatcgtgctc tacgacaagg gcgggcgtcc ggagctcgcc gagaaggagc
     3601 ggggcgagat cgccatcatc gagcgcttcc tgccgactca gctctccgat gaagcgttgg
     3661 aagccgccgt caaggagacc attgccgcca ccggcgccca gggcatgaag gacatggggc
     3721 gcaccatgac ggcgctgcgc gagcgctatg ccgggcagat cgacggggcc aaggcaagcc
     3781 aggtggtgaa gcggctgctc gggtgagctt tcagcgtcat cgcggtcccc tgtctttccg
     3841 acgctcgtcc gatggagtag catcgagcga ttgaattatt gcccgcggcg cgccagagct
     3901 gatggctgac agctgaaagc tgacggcttc gatggccttt ccaccccggt tcctcgacga
     3961 gttgcgcgcg cgagtctcgc tggcggaggt ggttgggcgc cgcgtccggc tcatccggcg
     4021 cgggcgcgaa tatgtcggcc tctgcccctt ccacaaggaa aagacgccgt ccttcagcgt
     4081 cgtcgaggac aagggcttct atcactgctt cggctgcggc gtgcatggcg acgtcatcgg
     4141 ctttgctatg cagaccgaga gcatgacctt ccccgaggcg gtcgaggcgc tggcgcgcca
     4201 ggccggtctc gatgtgccga cgcagagccc cgaggaccgc gagaagatcg agcgccaggc
     4261 gacgcttcag gcggcgatgg aagcggcgtg cgccttcttc gaagagcagc tgcacgccgt
     4321 gccggggcgc gacgcgcggc tctatctcga aggtcgcggc gtcgatctcg ccaccttgcg
     4381 gcgcttccgt ctcggatttg cgcccgaccg gcgcgacggt ctgttgcgcg ccctcgccaa
     4441 gcgctttccc gaagcgctgc tcatcgaggg cggactcgcc cggcgctcgg aggaaggcga
     4501 aagcttcgac tatttccgtc ggcgcatcat cttcccgatc ggcgaccggc aggggcgcat
     4561 catcgcgttc ggcggccgcg tgctcggcga aggccagccc aaatatctca attcgccgga
     4621 cacgccgctc ttccagaagg gacgcgtcct ttatggctgg gcgccggcgc gcgcggcggc
     4681 ggcgcgcgcg ccttcggcca tcgtcaccga aggctatatg gatgtcatcg cgctgcaccg
     4741 cgcgggcttt accacggccg tggcgccgct cggcaccgcg ctcacggaaa tgcagctcga
     4801 agagctgtgg cgcctcgccc ccgagcccgt gctgtgcttc gacggcgacg ccgcgggtct
     4861 gcgcgccgcc ggacgcgcgc tcgagcgcgc gctgccgctg ctgaagccag gtcagagcct
     4921 cacattcgcg acgctgccgg aaggcgagga cccggacacg ctgatcctgc ggcacggcgt
     4981 cggcgccatg accgaggtgc tggagcgcgc gcggccgctt gccgacgtgc tgtgggagct
     5041 cgagacggcg cgtccttgcg tgacgcccga gcaacgcgcg gcgctcgagc gccgcctcga
     5101 agagcgcgcg cgtgccatcg cggaccgttc tgtgcaggag cattaccgca gcttctttcg
     5161 ttcgcggctt ttcgatgcgc tgcggcgcaa caacaccggc gcgcgccggt ggcggccggg
     5221 tcggccggcg gggcccaatg tcggggtcca gctcaagacg ccggccgcgc tcgaggccgc
     5281 gccgcgcagc gagccgacgc cgttgcgccg ccggcgccag gagatcctgc tcgccctttt
     5341 gctcaatcac cccttccttc tgagcgatgt ggcagaggaa ttagcagaaa ttcagccggg
     5401 acccccagac cttgacaagc ttcgccacga aatcttaaga cttcacgccc ttaatccgga
     5461 ccttgacgct gcgacgctca ggctccactt gactaagagc ggctatgaca gggtcgttga
     5521 aggggtactg tcatcgcagg ttctggccca tgccgcgttt gcgcgggcgg gcgcagacgc
     5581 agagacggtg aggctgggat gggccgatac gagagaccag ctccgacagc cgcatctgcg
     5641 gacccagatc gccgaggcgg aacgggagct ggcggaggag accacggaag agaactgggt
     5701 tcgcttgcat ccattattgg agcagaagat cgaggatgac ggcgccacgg gaggcgggag
     5761 ccgttgaccg gggccgaaag gccggagcgg gcgcagcgga ggatggatcg caggaaccgg
     5821 acgcgcgagc gatgaaccgc ggcgcggccg gtcgtcgcat ttgagtgacc tagggcgggg
     5881 cagagatggc gagtaaggcg gcgaatgcgg cggaggcgcc ggagacgcgc gaggaagcgg
     5941 cggaagcgcc gttgctcaat accgtcgaac aggcgatccg gaagatggtc gcccgcggca
     6001 aggagcgggg ctacgtcacc ttcgacgagg tgaacgcggc attgccgacg gagcaggtgt
     6061 cgccggagca gatcgaagac accctcgcca tgctcaacga gcaaggcatc aacgtcgtcg
     6121 aaagcgaaga gacggacgag gccgccgcga ccgaaggggg agacggcgag aacgaagtac
     6181 gcgccggcaa tctcgacgac gaggacatcg ggcggaccga cgatcccgtg cgcatgtatc
     6241 tgcgcgagat gggctcggtg gagctgttgt cgcgcgaagg cgagatcgcc atcgccaagc
     6301 gcatagaggc cggccgcgag atgatgatcg gcggcctctg cgagagtccg ctcaccatgc
     6361 gcgcgctcat cgcctggcgc gatgcgctca ccgaaggcaa gatgctgctg cgcgacgtga
     6421 tcgatctcga cgccacctac ggtagcgcgt tcgccgaagc ggtcgaggag gtcgcggccg
     6481 aagtcgaagc cgaggccgag gccgccgcca ccaccaccac caccgaagcc gacgccgtgg
     6541 aagagccggt cgtcccggta cccgaagccg gcaacggcca ggatccgagc gcggtggcgc
     6601 tgccgccgcc ggcgcccggt gaagaggatc tcgacggcga agacacctcc atctcgctcg
     6661 cggccatgga agccgagctc aagccgagcg tgctcgcgac cctcgacggc attgccgcgc
     6721 tctacaagcg cctgcagaag gtgcagggcc agcgcctgac gaccctccag gaaggcgagg
     6781 agctcgcgcg tccgcaagag aagcgctacg acaagctcaa gagcgagatg gtcgacgcga
     6841 tgaagagcgt gcgcctcaac aatgggcgca tcgagaatct cgtcgggcag ctctatgcgt
     6901 tgaaccgccg gctcgtcgcc gcggagggca gactgctgcg gatggccgaa tccagcggcg
     6961 tcaagcgcca ggacttcctc aatcatcata tgggtcagga gctcgccccc gactggacga
     7021 cacgcgtcaa gcggctgcat ggcaagggct ggggctcgct cgtcaacaag cacggcaccg
     7081 aggtcaagac gctgcgcggc gaagtcgcgg ccatctccga ggaagcgggg ctgccgccga
     7141 tcgagttccg ccgcatcgtc tcgacggtgc agcgcggcga gcgcgaggcc gggcgcgcca
     7201 agaaggagat ggtggaggcc aatttgcgcc tcgtcatctc catcgccaag aaatacacca
     7261 atcgcggctt gcaattcctc gacctgatcc aggaaggcaa catcggcctg atgaaggcgg
     7321 tcgacaaatt cgagtaccgc cgcggctaca aattctcgac ctatgcgacc tggtggatcc
     7381 gacaggcgat cacgcgctcg atcgccgacc aggcgcgcac catccgcatc cccgtgcaca
     7441 tgatcgagac gatcaacaag ctggtgcgga cgtcgcgcca gatgctgcac gagatcggcc
     7501 gcgagcccac gcccgaagag ctcgccgaga agctcgccat gccgctcgag aaagtgcgca
     7561 aggtgctgaa gatcgccaag gagccgatca gcctcgagac gccgatcggc gacgaggagg
     7621 attcgcatct cggcgatttc atcgaggaca aaaacgcgat actgccgttg gacgccgcga
     7681 tccaggccaa tctgcgcgag acgacgacgc gcgtgctggc gtcgctcacg ccgcgcgagg
     7741 agcgcgtgct gcgcatgcgc ttcggcatcg gcatgaacac cgaccacacg ctcgaagaag
     7801 tgggtcagca attctcggtg acgcgcgagc gcatccgcca gatcgaggcg aaggcgctcc
     7861 ggaagctcaa gcacccgagc cgctcgcgca agctcagaag cttcctcgat acctgaatca
     7921 gaagacaggc atcagatatc agacggcgca ttgcgcagtg ctggccgtca ttgcgggcga
     7981 tccggcgcca ccaccagaac tgtgaagctg ccgggcagcg ctgcgggacg agcagcgccg
     8041 atagcgggtg cgtattgcgc tgacggcagg tagatccggc ccgttgcggg atcgagcgcg
     8101 gcggtgcgcg cgcccttcgc tgtctcgacg cgctctatga tttcaggcgt cggcgacagg
     8161 cgtatcacgg ccagtgtgcc gtcacctccc gacggaacaa aagccagatg ccgctgcgca
     8221 tcgtagagcg ctccgtccgg tcgcggtccg atggccaggc tggccatttc gtgcccgtcc
     8281 ggcgcggaca ccttggcgac accgttggcg caggcggaga cgatcagccg cacatccggc
     8341 gcgtaagcga tgccggttgg ccgatcgcag cccatcaacg gaaagcgggt cacgagctgg
     8401 cgcgttcgcg tgtcgatcac ggcgacgtcg ttctggtcct cgacattgac atagagccgg
     8461 ccctcgccgt cggcggcgcc cagctcgagc gaccctccga ccgcgaccgt cgccaccacc
     8521 tgtgcaatcg ctgggtcgat gacgctgatg tcgccggagc cggggttcat cacccaaagc
     8581 gtgcgcgtcg ccgggtcata cgcgacggcg tcgggattct ttccgaccgc caaactggcg
     8641 acgatctctc ccgtcatgcc ttcgaagatg gtggcggtat tggctccgcc gctggtcgag
     8701 aggacgcgcg tggagtccgg tatcggcaag gcggcgtggc cgcgcgtgct cgggacaagg
     8761 tttggcgtga ccttgccggt cgcgagatcg accgccatga cgccctcgct tcgggcgaca
     8821 taaagacgcc ccgacgccgc atcgacaccg gcgagatccc acccgccatc cgggccggcg
     8881 attcgcgaga ccacgcgata ggacggtgcc gtcggcgcct gagccgccgc cgcgccggcc
     8941 agcaggagca gggtcagggc gacgagaagg cagttaacgg cgggccaatt catcggctat
     9001 atgctcgcat ccttccgcag aggcccggcg catcttagac gacgctgccg cgtccgccca
     9061 gcacttcgga agacgggatc ggcgcgtcct cgcggcgcgc agactcagcc ggcggcttcg
     9121 gtcagcaggg cctcgaccct ggagtcgagt tcccgcgcca ccttgagcgc atccgggtta
     9181 ccgtagcctt cgaggaagct tgccatcttg tcatacgaga gatgcacgcc gtccggacgt
     9241 tcgtcgatga ggatggtcac cggagcgtaa gagccggcat cggcgacgtg cttcaccatt
     9301 tccttcatga tcagcggatt gccgatgacc aagcgcagca ccttcggacc ttggcgtccg
     9361 ctttcccgcc gaaggatcga tccgagatcg aagcggatga actccatgaa cccggaggtc
     9421 ccgagcgcgc gctggatcgt ctgctgcatc gcgccgaagc tcgtcgctga cccgatttcc
     9481 ttgttgaact ccgccatatt cgggcgcccg acgaccgctt cgagggcggc gacgacgatt
     9541 tcgaacggcc tcgacgacgt cacgctcacg cgctctacga tgatccggct ggtcatcgga
     9601 acactcctca cgagtttgcc tcagcgcgtg ccttctggat cgcggtccag atcttgagcg
     9661 gcgtgagcgg catatcgaga tggccgatcg cgaaaggcgc gagcgcgtcc agcacggcgt
     9721 gcacgatcgt cggcggagcg ccgatggcgc cgctttcgcc gatccccttc gcgcccaagg
     9781 gattggtcgg cgacggcgtc tcctggaatt gcgttcttat gttcggcaca gaatccgcac
     9841 gcggcaccgc gtagtcaagg agggagccgg tcatgagttg accggtctct ccgtcgtagc
     9901 agacttcctc gtaaagcgcc tgtccgatgc cctgcaccac gccgccgtga atctgcccgg
     9961 cggcgaggag gggattgatg atggtgccgg cgtcatcgac ggcgacatag tcgaggatct
    10021 ctaccgtgcc ggtttcaggg tcgacttcga cataggccat gtggactccg gagggagcgc
    10081 tcattcccgt gggatcatag aagagcgttt catcgagccc gggctcgacg tcattgggaa
    10141 gggcgtggcc gacataagcc atccgtgcca cgcgggcgaa actcagcggc gcgatgtcgg
    10201 tgcctgccac acggaaatgg ccgcgctcat agaggacatc cggctcagcg acctccatca
    10261 tggccgcggc gatcttgcgc gctttcgcga cgatgcgtct cgcgcaaacc ttgacgctcg
    10321 agccgccgac cgggatggag cgcgagttga aggtgccatg gccggcctgc acctgccgcg
    10381 tatctccctg caccacctcg atatgctcga ccggcagctg cagctcgtcg gcggcaatct
    10441 gcgcgaatac cgtcgcatga ccgtgcccct ggctcatgga gccggagaaa agcgtcactt
    10501 tcccgctcga atcgacgttg acacgcgcgc tctcccagcc accgcgatca aacccgctgg
    10561 ccgccagacg gcgcgacggc gccatgccgc acatctcggt ataggcggcg acgccgatgc
    10621 cgcgataccg gccgctgccg cgcagcgcgc gctgctcctc gcgccgcgcc gcatagtcg
//
LOCUS       DASYNS010000591         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5454358, whole genome shotgun sequence.
ACCESSION   DASYNS010000591 DASYNS010000000
VERSION     DASYNS010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5454358"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2005)
                     /locus_tag="VGP50_17960"
     CDS             complement(<1..>2005)
                     /locus_tag="VGP50_17960"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HEV8029324.1"
                     /translation="PLDELDFGAQASLSGVATHATAQLGAHVDLAPDAATPVTVDLFT
                     GTLEVRGLTVQYFEHLPPLRGVNGTGTFDRTRLDLTATGGAVEGVQLVSGTAKLSKLD
                     THDEEIAIDVAAKGPLRDIMEVLDYDPLHYAQKLDVAPAAIGGAAEGHVLFNFPLKRD
                     LPLDELDFGAQASLSGVAIAKIVGGRDLSDGVFQLKLDRTAIKLDGTGRIADVPASLS
                     WVQSLKTGEGDHMRATMKARVDDAGRQRLDVDFFPDMLKGPVDADVAYTTVEPKRAAA
                     TVSLDLKDATVEIAKLNWTKPSGVAATAALDLELVDGHVRNIKEATLKGGGADARVSV
                     AFDAQQDLRRFDAPRLLVGETDASGSVGRRPEGGWQVDLHGLAFDATGLVANLDSGSS
                     GALDEPLVIDATLDRLVLGRKREARDVRAQVYYDGAHWQAASFDASLFGGGKARLRFG
                     ESRGKRNLQLTTDDFGGLMKLTNISQNIAGGQLQITGEVEDQGEKRILRGKVDGADYR
                     LVNAPLLARLLSVASFSAIGSLLSGEGIPFTRLKADFTLSDGKIDVAVARAYGGAIGL
                     NVSGSFDTSTESLDISGTLVPAYTINSVLGNIPVLGPILMGGQGEGIFGANFKIAGPV
                     ANARISVNPLSALAPGALRKLFLFEAPDIAEPAPPPSASGERLQ"
BASE COUNT          315 a          671 c          699 g          320 t
ORIGIN      
        1 actgcaggcg ctcgccgctc gccgagggag gtggggcggg ctcggcgatg tcgggggctt
       61 cgaagagaaa gagcttgcgc aacgcgcccg gcgccagcgc gctcagcgga ttgaccgaga
      121 tccgcgcatt cgcgaccggg ccggcgatct tgaaattggc gccaaagatg ccttcgccct
      181 gaccccccat caggatcggc cccagaaccg ggatgttgcc gagcacgctg ttgatggtat
      241 aggccgggac gagcgtgccc gagatgtcga gcgactcggt gctcgtgtcg aagctgccgc
      301 tgacgttgag gccgatggcg ccgccatagg cgcgcgccac cgcgacgtcg atcttgccgt
      361 cgctcaaggt gaagtcggcc ttgagacgcg tgaagggaat gccctcgcca gacagcagcg
      421 agccgatggc ggaaaaggat gccaccgaca gcaggcgcgc gaggagcggc gcgttgacga
      481 gacgataatc cgcgccgtcg accttgccgc gcaggatgcg cttctccccc tggtcctcga
      541 cctcgccggt gatctggagc tggccgccgg cgatgttctg ggagatgttg gtgagcttca
      601 tcaagccgcc gaaatcgtcc gtggtgagct gaagattgcg tttgccgcgg gattcgccga
      661 agcgaagccg cgccttgccg ccgccgaaga ggctcgcgtc gaacgacgcc gcctgccagt
      721 gcgcgccgtc gtaatagacc tgcgcccgga cgtcgcgcgc ctcgcgcttg cggccgagga
      781 cgaggcggtc gagggtcgcg tcgatgacga gcggctcatc gagagcgccg ctcgagccgc
      841 tgtcgagatt ggcaacaagg cccgtcgcgt cgaaagcgag gccatgcagg tccacttgcc
      901 agccgccttc cgggcggcgc ccgacgctgc cgctggcgtc ggtctcgccg acgagcaagc
      961 gcggcgcgtc gaaacgccga aggtcttgct gcgcgtcgaa agcgaccgag acgcgcgcat
     1021 cggcgccgcc gcctttgagc gtcgcctcct tgatgttgcg cacatggccg tcgacaagtt
     1081 cgagatcgag cgccgccgtc gccgccacgc ccgacggctt ggtccagtta agcttggcga
     1141 tctcgaccgt ggcgtccttg agatccagcg acaccgtcgc cgccgcgcgt ttcggctcga
     1201 ccgtggtgta ggcgacatcg gcatcgacgg gccccttcag catgtcgggg aaaaagtcga
     1261 catcgagccg ctgccgcccc gcatcgtcga cgcgcgcctt catggtggcg cgcatatggt
     1321 ccccctcgcc ggtcttcagg ctctgtaccc agctcaggct cgcgggcacg tcggcgatcc
     1381 gccccgtgcc gtccagcttg atcgcggtgc ggtcgagctt cagctggaac acgccgtcgc
     1441 tcaagtcgcg tccgccgacg atcttggcga tggcgacgcc gctcaggctc gcctgcgcgc
     1501 cgaaatccag ctcatcgagc ggcaggtcgc gcttcagcgg gaaattgaag aggacatgac
     1561 cctcggccgc gccgccgatg gcggcaggcg ccacgtcgag tttctgcgca taatgcagcg
     1621 ggtcgtagtc gagcacctcc atgatgtcgc gcaacggccc cttggcggcg acgtcgatgg
     1681 cgatctcctc gtcatgggtg tcgagcttcg agagcttggc ggtgccgctc acaagctgca
     1741 cgccttcgac ggcgccgccg gtcgcggtca ggtcgaggcg cgtgcggtcg aaagtgcccg
     1801 tcccattgac gccgcgcagc ggcggaagat gctcgaaata ttgcaccgtg aggccgcgga
     1861 cctcgagcgt gccggtgaac aggtcgaccg tcaccggcgt cgcggcgtcg ggcgcgagat
     1921 cgacatgcgc gccgagctgc gccgtggcgt gggtggcgac gccgctcagg ctcgcctgcg
     1981 cgccgaaatc cagctcatcg agcgg
//
LOCUS       DASYNS010000592         7868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4364248, whole genome shotgun sequence.
ACCESSION   DASYNS010000592 DASYNS010000000
VERSION     DASYNS010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4364248"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..951)
                     /locus_tag="VGP50_17965"
     CDS             complement(184..951)
                     /locus_tag="VGP50_17965"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEV8029325.1"
                     /translation="MTIRLYGSWASPFARKVALALELKGLGYEPIDALARAKHDELVE
                     VNPRAEVPALVDGGVTVVNSSDILQYLDWRYPEPALYPAAIEDRVTARALEREVDTWF
                     DAILVACSLWLWAERDDMPPAGLHEAGQKDLDVILARLERFLAARPTPWPFIEPGLLE
                     CAWFPHLAAVKPFGLVIDGARFPATVAWFQAMRGHPVFRADAKRTATFMTQFIREGTH
                     ERRRIFWRGDRIEWLLSRGFHRWLVTEIEADRAAFPS"
     gene            complement(1116..4625)
                     /locus_tag="VGP50_17970"
     CDS             complement(1116..4625)
                     /locus_tag="VGP50_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HEV8029326.1"
                     /translation="MIDLVLGHGLAFEDLYRRDGLARLDAAFVAHLCEGDVALHNRLM
                     TARRDPVALDRKAESDLLIELAPHLEDFVAGLFGIAGEVRALQARHQSLAALYAVKRL
                     FVQRRAVKGMTPETAAALDGPALALALERLMGETLDEASFARHVTRWLEAEAGHAASL
                     ETAQRYAAWATLAPEGRKKHKKGVLFKLPHRLEMTHLVPVETIVEHGVAMLRLPETEW
                     RYREGFALTDAGTDLTGALDQANYCIWCHHQAKDSCSRGLKDKKTNAFQKTVFGVTLA
                     GCPLEEKVSEMNEVKAQGFSIGALAIVAIDNPMMAATGHRICNDCMKACIYQKQEPVD
                     IPQIETRNLKDVLSLPWGFEIYSLLTRWNPLDIRRPLPKPRSGYKVLVVGLGPAGFTL
                     AHHLMNDGHTVIGVDGLKIEPLPEALAGVGPDGGRRPFRPVRDVADLTEELGDRVMAG
                     FGGVAEYGITVRWDKNFLKMVRLLLERRAEFAMFGGVRFGGTLGIDSAFALGFDHIAL
                     CAGAGRPTVIAMKNGLARGVRQASDFLMALQLTGAAKTDSVANLTLRLPVVVVGGGLT
                     AIDTATESLAYYPLQVEKFLSRYETLVAERGAAAVRAGWSEEERATADEFILHARALR
                     AERALAERDGRAPRLVDLMNAWGGVTIAYRRRLIDAPSYTLNHEEVAKAMEEGIRFAE
                     CVVPEAVEVDAYGHAAALVLQRHKLDEALKQLVATDERLTLPARSILIAAGTQPNTVL
                     GREDPANVVLDGRYFQAYDESGRPAKPERVAKPETVQVLMSLRPDGRAISFFGDLHPS
                     FNGNVVKAMGGAKQGYPVVSRMLAKRAAAAPAPDVLVARLNDELRPRVHHVERLTPTI
                     VEVVVKAPMAARAFHPGQFYRLQNYETHGARIDGTALAMEGLALTGAWVDREKGLLST
                     IVLEMGGSSDLCTLLEPGEPVILMGPTGTPTETPAGETVALVGGGLGNAVLFSIGQAL
                     RAQGSKVLYFAGYKKLQDRYKVEEIEKAADAVVWCCDEAPGFAAARPQDRAFVGNIVE
                     AMLAYGTGRLGAAPLALGSVDRVIAIGSDGMMAAVGKARHGVLAPYLKRDHVAVASVN
                     SPMQCMMKEICAQCLQVQKDPTTGAETVVFSCFNQDQPLDHVDFRALRERLGQNSLQE
                     KLTKLWIDRCLHHLGARTAAAAE"
     gene            complement(5046..5429)
                     /gene="uraH"
                     /locus_tag="VGP50_17975"
     CDS             complement(5046..5429)
                     /gene="uraH"
                     /locus_tag="VGP50_17975"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF012785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEV8029327.1"
                     /translation="MDLEPKTQSAGVLTTAVIDAEAGIPAAAMLIDLFRVARDIGERQ
                     HLRTVETDAQGAIGEPLLEGASLEPATYELLFHVGRYFKARRASLDEGPFLNVVPVRF
                     SIADPGHRYHVTLLAGPWSYSVYRS"
     gene            5724..6575
                     /locus_tag="VGP50_17980"
     CDS             5724..6575
                     /locus_tag="VGP50_17980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004273577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV8029328.1"
                     /translation="MEPGRPSVTAQGAAMLRAAHQLLDTPRIFDDPLALAIIGTEAEA
                     ALRADPGSFQTPYRRRQRANIAARSRHAEDALAKARRRGVRQYVILGAGLDTFAYRSA
                     SEDGALGVFEVDHPATQAWKRQRLAERGMAATRRLTYVPIDFERERLEDALRDAGLDM
                     DEPVFISWLGVATYLTRDAVFQTLRSVAGLTPGSAIIFTYAQSPETLNPEQRAMFDAA
                     AERVAALGEPWRSFFEPVALAEDLRAVGFAAVEDLGPAEIHARYFAGRSDGFAPGSTG
                     HLALAAL"
     gene            6572..7027
                     /locus_tag="VGP50_17985"
     CDS             6572..7027
                     /locus_tag="VGP50_17985"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HEV8029329.1"
                     /translation="MTTREAFSYRRDPAVPPFPDERPILIFDGKCVLCSGFARFILRA
                     DRHRRFRLLAAQTPLGAALYRHFALAPVDYETNILIENGAAWLKSEGSIRIFERLGLP
                     WSLAAVARLLPRPLRDRLYDVVARNRLRWFGTRQTCYLADPSEADRFLA"
     gene            7024..>7868
                     /locus_tag="VGP50_17990"
     CDS             7024..>7868
                     /locus_tag="VGP50_17990"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEV8029330.1"
                     /translation="MSDGLRLLIIGGYGIFGGRIVQLLEDEQRLTLVVAGRSLERAHA
                     FCRSRVNARAALMPAAVDRSGDVAAQIEALTPDLVVDASGPFQSYGAEGYRVIAACIA
                     RGVNYLDLADGSDFVAGVGAFDAQARAAGCFVLSGVSSFPVLTAAVIRRLSSGMARID
                     RIRGGIAPSPFAGVGENVIRAIAGYAGKPATLRRNGAPGIAYPFTEQMRFTIAPPGRR
                     PLAHTMFSLVDVPELRALPELWPEARDIWMGVGPVPEILHRALVALAWLVRAKLLPTL
                     APLTPL"
BASE COUNT         1183 a         2695 c         2693 g         1297 t
ORIGIN      
        1 agagcgacgg agagcccttc tcccgcgagg agcgggagaa ggtgtcgcgg gcgcagcccg
       61 cgacggatga gggggcgccc gacgcgcgtg ccacgcccga tgggcaccct caccctccca
      121 ctgcttcgct gaagcttcgc agtgggtccc tccctctccc gctctcgcgg gcgaggggga
      181 atttcagctc gggaacgcgg cgcggtcggc ttcgatctcg gtcacgagcc agcgatggaa
      241 gccgcgcgac aacagccatt cgatgcggtc gccgcgccag aagatgcggc gccgctcatg
      301 cgtgccctcg cggatgaact gcgtcatgaa ggtggcggtg cgtttggcat cggcgcggaa
      361 caccggatgg ccgcgcatcg cctggaacca ggcgacggtc gccggaaagc gcgcgccgtc
      421 gatgacgagg ccaaagggct tcaccgcggc gagatgcggg aaccaggcgc attcgagaag
      481 gcccggctcg ataaagggcc agggcgtcgg ccgcgccgcg agaaatcgct cgagccgcgc
      541 gaggatgacg tcgaggtctt tttgccccgc ctcatggaga cccgccggcg gcatgtcgtc
      601 acgctccgcc cagagccaga gagagcaggc gacgagaatg gcgtcgaacc aggtgtcgac
      661 ctcgcgctcg agcgcccgcg ccgtcacgcg atcctcgatg gccgcgggat agagcgccgg
      721 ctcgggatag cgccaatcga gatattgcag gatgtcgctc gaattgacga cggtcacgcc
      781 gccatcgacg agcgccggca cctcggcgcg cggattgacc tcgacgagtt cgtcgtgctt
      841 cgcgcgcgcc agagcatcga tgggctcata gccgaggccc ttgagctcga gcgccagcgc
      901 gaccttgcgc gcgaatgggc tggcccagct tccatagagg cgaatggtca tcggcatctc
      961 cgctgctgcg tgctgcaatg atgctgcggg tggggcgtgt cggaagcaaa tgcatattcc
     1021 cgagggggat atgctgggac gtcattcttg gcgagcggtg cgcgcgtgtc gacggtcccc
     1081 tcatcctccc gcggtgcggg gagagcggaa agtcgctact ccgcggcggc ggcggtgcgg
     1141 gcgccgaggt ggtggagaca gcggtcgatc cacagcttcg tcagcttctc ttgaagggag
     1201 ttctggccga gacgctcccg gagggcgcgg aagtcgacgt ggtcgagcgg ctggtcctgg
     1261 ttgaagcagg agaacacgac cgtctcggcg ccggtcgtgg gatccttctg cacctgcagg
     1321 cactgcgcgc agatctcctt catcatgcat tgcatcggcg agttgacgga ggcgacggcg
     1381 acgtggtcgc gctttaagta tggcgccagc acgccgtggc gcgccttgcc caccgccgcc
     1441 atcatgccgt cggagccgat ggcgatgacg cggtcgacgg atcccagcgc gagcggcgcg
     1501 gcgccgagcc tgcccgtgcc ataggccagc atcgcctcga cgatgttgcc gacgaaggcg
     1561 cggtcttgcg gccgcgccgc cgcgaagccc ggcgcctcgt cgcagcacca caccacggcg
     1621 tcggcggcct tctcgatctc ttcgaccttg tagcggtcct gcagcttctt gtagccggcg
     1681 aagtagagca ccttcgagcc ctgcgcgcgc agcgcctggc cgatcgagaa cagcacggcg
     1741 ttgccgaggc cgccgccgac cagcgccacc gtctctcccg cgggcgtctc cgtcggcgtg
     1801 ccggtcggcc ccatcaggat gaccggctcg cccggctcga gcagcgtgca gagatcggag
     1861 gagccgccca tctccagcac gatggtcgag agcaggccct tctcgcgatc gacccaggcg
     1921 ccggtgagcg ccaggccttc catcgccagc gccgtgccgt cgatgcgcgc gccgtgggtc
     1981 tcatagttct ggaggcgata gaactggccg ggatggaagg cgcgcgccgc catcggcgcc
     2041 ttcaccacca cctcgacgat ggtcggcgtc agccgctcca cgtgatggac gcgcggacgc
     2101 agctcgtcat tgagccgcgc caccagcacg tcgggagcgg gcgcggcggc cgcgcgtttc
     2161 gccagcatgc ggctcacgac gggatagccc tgcttcgcgc cgcccatggc cttcacgaca
     2221 ttgccgttga aggacggatg gaggtcgccg aagaagctga tggcgcggcc gtcgggccgc
     2281 aagctcatca gcacctgcac cgtctcgggc ttggcgacgc gctcgggctt cgccggacgg
     2341 cccgactcgt cataggcctg gaagtagcgg ccgtcgagca cgacattggc cgggtcctcg
     2401 cggcccagca cggtgttggg ctgcgttccc gcggcgatca ggatgctgcg cgccggcagc
     2461 gtcaacctct cgtccgtcgc caccagctgc ttcagcgcct cgtcgagttt gtgacgctgc
     2521 aggaccagcg ccgcggcatg gccataggcg tcgacctcga ccgcctcggg caccacgcat
     2581 tcggcgaagc ggatgccctc ctccatcgcc ttcgccactt cttcgtggtt gagcgtgtag
     2641 gagggcgcat cgatgagccg gcggcgatag gcgatggtga cgccgcccca ggcattcatg
     2701 agatcgacga ggcgcggtgc gcggccgtcg cgctccgcca gcgcgcgctc ggcgcgcaag
     2761 gcgcgggcat ggaggatgaa ctcgtccgcg gtcgcacgct cctcttcgct ccagccggcg
     2821 cgcaccgccg ccgcgccgcg ctccgccacc agcgtctcgt agcgcgatag gaacttctcc
     2881 acctggagcg ggtagtaggc gagcgactcg gtcgccgtgt cgatggcggt gaggccgccg
     2941 cccaccacca ccaccggcag gcggagcgtc agattggcga cgctgtcggt cttggccgcg
     3001 ccggtgagtt gcagcgccat gaggaagtcg gaggcctggc gcacgccccg cgccaggccg
     3061 ttcttcatcg cgatcacggt tggccgcccg gcgccggcgc agagcgcgat gtgatcgaag
     3121 cccagcgcaa aggcgctgtc gatgccgagc gtgccgccga agcgcacgcc gccgaacatg
     3181 gcgaattcgg cgcggcgctc caggagcagc cgcaccatct tgaggaagtt cttgtcccag
     3241 cgcacggtga tgccgtattc ggcgacgccg ccgaagccgg ccatgacccg gtcgcccagc
     3301 tcctcggtga gatcggcgac atcgcgcacg ggacggaacg gccgccgccc gccgtcggga
     3361 ccgacgcccg ccagcgcttc cggcaagggc tcgatcttga ggccgtcgac gccgatgacg
     3421 gtatgcccgt cattcatcag gtgatgggcg agcgtgaagc cggcgggacc caaccccacc
     3481 accagcacct tgtaccccga gcgcggcttc ggcagcggac ggcggatgtc gagcgggttc
     3541 cagcgcgtca ggagactgta gatctcgaag ccccagggca ggctcagcac gtccttcaag
     3601 ttgcgcgtct cgatctgcgg gatatcgact ggctcctgtt tctggtagat gcaggccttc
     3661 atgcagtcgt tgcagatgcg atggccggtg gcggccatca tcgggttgtc gatggcgacg
     3721 atggcgaggg cgccgatgct gaagccttgc gccttcacct cgttcatctc ggagaccttc
     3781 tcctcgagcg ggcagcccgc cagcgtcacg ccgaagacgg tcttctggaa ggcgttagtc
     3841 ttcttgtcct tgaggccgcg cgagcaactg tccttcgcct ggtgatgaca ccagatgcag
     3901 taattcgcct ggtcgagcgc gcccgtgagg tcggtgccgg catcggtgag cgcaaagcct
     3961 tcgcggtagc gccactcggt ctcgggcagt cgcagcatgg cgacgccatg ctccacgatc
     4021 gtctccaccg gcacgagatg ggtcatctcg aggcgatgcg gcagcttgaa gagcacgccc
     4081 ttcttgtgct tcttccggcc ctcgggcgcg agcgtcgccc aggcggcgta gcgctgcgcg
     4141 gtctcgagcg aagctgcgtg ccccgcctcg gcctcgagcc agcgcgtcac atggcgggca
     4201 aagctcgcct cgtcgagcgt ctcgcccatc aggcgctcga gcgccagcgc cagcgccggg
     4261 ccgtcgagcg cggccgccgt ctccggcgtc atgcctttga cggcacggcg ctgcacgaag
     4321 agccgcttca cggcataaag cgccgccaga gactggtggc gcgcctggag cgcgcgcacc
     4381 tcgccggcga tgccgaagag accggcgacg aaatcctcga gatggggggc gagttcgatc
     4441 agcaggtcgg attcggcctt gcggtcgagt gccacaggat cgcgccgcgc cgtcatcagc
     4501 cggttgtgga gcgcgacgtc gccttcgcac agatgcgcga cgaaggccgc gtcgagccgc
     4561 gccagcccgt cgcgccgata aagatcctcg aaggcaaggc catgcccgag gacgaggtcg
     4621 atcatctcac gcactctccc gcgctgcccg ctcgccgatc gcggcgaggg ccctgtcccc
     4681 aggtgatcct gcccttgagc tttgtgcaga tcggcacgag ccagaccggc attggacggc
     4741 cgctgtatgt ctcgggcggc gcggcgcgtc aacccgatgt cgtgaggggg tattgccatg
     4801 gtccccaggg aatatcgaca ggccgaaaac ggaacgttgc cctctatatc aagggcggcc
     4861 ggaccttcga caagacgtcc ttatgtcgat cggagactga atgcaggcat actttgagcg
     4921 gctcgacatc gggctgtaac ttgcgcggga atcgcgcttt ccgacttttc ggagaagtga
     4981 aaaatcgccc gatattgcag ggtgttacgt agtcgagccg gcgacgacgc ttcgcctcgc
     5041 gccgcctagc tccgatacac gctgtacgac cagggccctg ccagcagcgt cacgtggtag
     5101 cgatgcccgg ggtcagcgat cgagaagcgc accggcacga cgttgaggaa agggccttcg
     5161 tcgagcgacg cgcggcgcgc cttgaagtag cggccgacgt gaaacagcag ctcgtaggtg
     5221 gccggctcga gcgacgcgcc ttcgagcagc ggctcgccga tggcgccttg tgcatcggtt
     5281 tcaaccgtgc gcagatgctg gcgctcgccg atatcgcgag cgacgcggaa aagatcgatc
     5341 agcatcgccg ccgccgggat tccggcctcg gcgtcgatga cagcggtcgt caggacgccc
     5401 gcgctctgcg ttttcggttc aaggtccaat gcgctgaacg aacgggcctc tcgggtcggg
     5461 cgttcccttc atcgcgcata gtcgcgaaaa tcatcgtcgc ggtccgtgcg ggatgacgaa
     5521 gatcatcccg tgcgggagcc cgaagccgcc gaatccgttc ttgaggtcga tgcgggaaca
     5581 ccgtgttgct gcctgcggtt gcgtatttgt gacgcaccga tgtcgggaaa gttgcgcccc
     5641 ggctcgcctg ggcgcagctt gacgctgcca cgctggcggc ggcacaattc tcgcctcgag
     5701 gtttcgcaag aaggatcatt ggcttggagc cgggtcggcc gagcgtcacc gcacagggcg
     5761 cagccatgct gcgcgcggcg caccagcttc tggacacgcc gaggatcttc gacgatccgc
     5821 tggcgctcgc catcatcggc accgaggccg aggcggcgtt gcgtgccgat cccggatcgt
     5881 tccagacgcc ttaccggcgc cggcagcgcg ccaacatcgc cgcccggagc cgccatgccg
     5941 aggacgcgct ggcgaaagcc aggcggcggg gcgtgcggca gtacgtgatc ctcggcgccg
     6001 gcctcgacac cttcgcctat cggagtgcct cggaggacgg cgcgctcggc gtcttcgaag
     6061 tggaccatcc ggcgacccag gcgtggaagc gccagcgcct cgccgagcgc ggcatggcgg
     6121 cgacgcgccg gctgacctat gtgccgatcg acttcgagcg cgagcgcctc gaggatgcct
     6181 tgcgcgacgc gggcctcgac atggacgagc ccgtattcat ctcctggctc ggcgtcgcca
     6241 cctacctgac gcgggacgcc gtcttccaga ctctgcgctc ggtcgcgggg ctgacgccgg
     6301 gcagcgcgat catcttcacc tatgcgcaat cgcctgagac gctcaacccc gagcagcgcg
     6361 cgatgttcga cgcggccgcg gagcgcgtcg ccgcgctggg cgagccctgg cgcagcttct
     6421 tcgagcccgt ggcgctggcc gaggatttgc gcgcagtcgg cttcgccgcg gtcgaggatc
     6481 tgggaccggc cgagattcac gcgcgctact tcgccggtcg ctccgacgga tttgcgccgg
     6541 gctccaccgg ccatctggcg ctcgccgccc tttgaccacc cgcgaagcct tcagctaccg
     6601 gcgggatccg gcggttccgc ctttcccgga cgagcgtccc attctcatct tcgacggcaa
     6661 atgcgtgctc tgctctggct tcgcgcgatt cattttgcgc gccgatcgcc atcgccgctt
     6721 ccggcttctc gcggcgcaga cgccgctggg cgcggcgctc tatcggcatt tcgcgctcgc
     6781 tcccgtcgac tacgagacga acatcctgat cgagaacggc gcggcctggc tcaaatccga
     6841 aggatcgatc cgcatcttcg agcgcctggg cttgccctgg tcgctggccg cggtcgcgcg
     6901 gctgctgccg cgccccttgc gcgaccgtct ctatgacgtc gtcgcccgca atcgcctgcg
     6961 ctggttcggc acgcgccaga cctgctacct cgccgatccg tccgaggccg accggtttct
     7021 cgcgtgagcg atggccttcg cctgctcatc atcggcggct acggcatttt cggaggccgc
     7081 atcgttcagc tgctcgagga cgagcagcgg ctgacgctgg tggtcgccgg gcgctcgctc
     7141 gagcgggcgc acgcgttctg ccgctcgcgc gtcaatgcca gggcagcgct catgccggcg
     7201 gccgtcgatc gcagcggcga tgtcgcggcg cagatcgagg cgcttacgcc cgatctcgtg
     7261 gtcgatgcca gcggaccgtt ccagtcctat ggcgcggaag gctatcgcgt catcgcggcg
     7321 tgcattgccc gaggcgtaaa ttatctcgac ctcgccgacg gatcggattt cgtcgccggt
     7381 gttggcgctt tcgatgcgca ggcgcgcgcg gcgggctgct tcgtgctctc gggcgtctcg
     7441 agctttccgg ttctcaccgc cgccgtcatc cgccgcttgt cctcgggcat ggcgcgcatc
     7501 gatcgcatcc gcggcggcat cgcgccctcg ccctttgccg gcgtcggcga gaatgtcatc
     7561 cgcgcgatcg ccggctatgc cggcaagccc gccacgcttc gccgcaacgg cgcgccgggc
     7621 atcgcctatc ccttcaccga gcagatgcgc ttcaccatcg cgccaccggg acgccggccc
     7681 ttggcccaca cgatgttctc gctggtcgat gtcccggagc tcagagcgct gccggagctc
     7741 tggcccgagg cgagagacat ctggatgggg gtgggcccgg ttccggagat tctgcatcgc
     7801 gcgctcgtcg cgctggcgtg gctggtgcgc gcaaaactgc tgccgacgct cgcgccgctg
     7861 acgccgct
//
LOCUS       DASYNS010000593         4830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2336941, whole genome shotgun sequence.
ACCESSION   DASYNS010000593 DASYNS010000000
VERSION     DASYNS010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2336941"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..546)
                     /locus_tag="VGP50_17995"
     CDS             complement(325..546)
                     /locus_tag="VGP50_17995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029331.1"
                     /translation="MRELAQQGPQSAGIEAGAFHDRAHDVIGQQFIERRFSDHSVHSH
                     RKSPRVPSSRRKMSRNLGRTMATAGYFKE"
     gene            complement(566..1966)
                     /locus_tag="VGP50_18000"
     CDS             complement(566..1966)
                     /locus_tag="VGP50_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014247347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV8029332.1"
                     /translation="MVRLLRTNAFRLAAIYFGLFAVSVSALLVFIYFSTADFIEQQTE
                     VTIDAEITGLREQYRERGLAGLVEIIRARVAGQHSDTSLYLITDATLQPLAGNISGWP
                     GAVPARPTRISFPVHSKSPAGDDSDTALAAVFPLPGGYLLLVGRDLHDARVFRSRITE
                     TLSWAALLTLALGVVGGLFMTRNMLRRVEAVNRTSTRIIHGDLSQRVPLTGSGDEFDQ
                     LAGNLNAMLDQIERLMAGMRQVTDNIAHDLRTPLARLRARLEVTLLERPDATLYAEAL
                     RETITEADRLLGTFNALLSIAEAEAGSRRQTMAVVDVAEIARAVAELYEPAAEEKGLR
                     LDIANGGVLPVRGDRHLLSQAIANLLDNALKYTPSGAVSLTTRESAEGARIEVADRGP
                     GVPADRREAVFDRFVRLEGSRSTPGNGLGLSLVRAVATLHGGSAWLEDNNPGVRAVIT
                     LPRVEAPALMSTRNAA"
     gene            complement(1966..2646)
                     /locus_tag="VGP50_18005"
     CDS             complement(1966..2646)
                     /locus_tag="VGP50_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV8029333.1"
                     /translation="MKLLVIEDDRAAAAYVAKGLSESGYVVDLAPDGRTGLFMASAGN
                     YDALIIDRMLPGMDGLALVTALRAAEIRTPVLILSALGAVDDRVKGLRAGGDDYLAKP
                     FAFAELLARVEALLRRGSSASTQPTKLKVGDLEMDLLTRNVTRGGQDIELLPREFHLL
                     EFLMRHAGQVVTRTMLLENVWDYHFDPQTNVIDVHVSRLRQKIDKGFAKPMLHTIRGA
                     GYSLRAGS"
     gene            2807..3232
                     /locus_tag="VGP50_18010"
     CDS             2807..3232
                     /locus_tag="VGP50_18010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV8029334.1"
                     /translation="MTMNELKDDVPARARRQPPSILIVEDEALVASYIREVLEESGFT
                     IAGIASSGPEAMSLAIASRPDLALVDIKLAGPMDGIEVAELMRSRFNTYSIFLSGVAD
                     PQTMERARNVDHLGFLEKPFRPSQVFNALQQALDKLPSH"
     gene            3473..>4830
                     /locus_tag="VGP50_18015"
     CDS             3473..>4830
                     /locus_tag="VGP50_18015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV8029335.1"
                     /translation="MRARRDLAPLWRLLMRLSMRLILAISFAALSAAAVAGHFVVLPA
                     LELATSLTALLLLAALAYALGLERGKRPVAGLADAMTRQGVELEHTRSAAGVTAASMR
                     HLFDGNPIPMYVYDQASLRFLDANDSAVAQYGHSRERFLAMTIEDIRCREEVARLRAS
                     RAGMIWSEGSAGIWEHRKADGTTISVEIIHRSILFQDCAAILVAAIDVTHACRVAREL
                     RDSQTRLKALADNLPGIVYQCRLTAGGEISYPFCSAAVSDLFGPGPDGDADFVVVTRS
                     ILPEDRTAFMTSVRRSAADLSPWSLDFRCRRRNGTISWFSGRGKPRRFADGETLWDSV
                     LIDISEQKNAEQALRESEAQLRRSREHLARAQRTADIGSWEIDLRSRSLEWSDELFRV
                     LGLSREQVAPGFDHMIARFCPEDRERMRGWLELVCAGASVAASEGRLLLPDGKERWVR
                     MDA"
BASE COUNT          791 a         1579 c         1635 g          825 t
ORIGIN      
        1 aatcgccgca ggagccggcg ttcgtcgccc ccgagagcgc tgccgcgctg ctcgagcgcg
       61 gcgagcaacc gctcgagcac ggtcagggtc ttctggatct cgagagtggt cataaccatg
      121 gcgcgctcct cttcgccctg acaaacgcga agattgaggc catggttccg atccacagac
      181 gcctgttgcc ccgcttcctg ggcgcgccgg acacaatgcc aaaaccccga tatagcgaga
      241 aattgatccg ctaaaagtcg atcgattcgt gacgggtcgg cgacgcgttt aggacggaac
      301 agcgcaacgc cggggcgcgg cgttctattc cttaaaataa ccggcagttg ccatagttcg
      361 gccaaggttg cgcgacatct tcctcctcga cgacgggacg cgaggagact tgcgatgcga
      421 gtgcacggaa tggtcggaga accgacgctc gatgaactgc tggccgatga catcatgcgc
      481 ccggtcatga aaagcgccgg cctcgatgcc ggcggattgc gggccctgtt gcgcgagctc
      541 gcgcatcgcc tcgcgccgga caatctcacg ccgcgttgcg ggtgctcatc agcgccggag
      601 cttcaacccg cggcagggtg atcaccgccc tgacgccggg gttgttgtcc tcgagccagg
      661 cggagccgcc atgcaatgtg gcgacggcgc gcaccagact gagacccagc ccattgcccg
      721 gagtgctgcg gctgccttcg aggcggacga agcggtcgaa taccgcctcg cgccggtcgg
      781 ccgggacgcc ggggccgcga tcggcgactt cgatgcgcgc cccttcggcg ctctcgcgcg
      841 tcgtcagcga cacagcgccg ctgggcgtgt atttgagggc attgtccagc aggttggcga
      901 ttgcctgcga cagcaagtgg cggtcgccgc gaacgggaag caccccgcca ttcgcgatat
      961 cgagccggag ccccttctcc tcggcggcgg gctcatagag ctcggccacc gcgcgcgcta
     1021 tctccgcgac gtcgaccacc gccatcgtct ggcgccgcga gccggcttcc gcttcggcga
     1081 tgctcaacag ggcattgaac gtgccgagga ggcggtcggc ctcggtgatc gtctcgcgca
     1141 gcgcctcggc gtagagcgtc gcgtccggac gctcgagcag cgtcacctcg agccgggcgc
     1201 gcagccgcgc cagcggcgtg cgcaggtcat gcgcgatatt gtcggtgacc tggcgcatgc
     1261 ccgccatcaa gcgctcgatc tggtcgagca tcgcgttgag gttgccggcg agctggtcga
     1321 actcgtcgcc gctgccggtg agcggcacgc gttgcgacaa gtcgccatgg atgatgcgcg
     1381 tgctggtgcg gttcaccgcc tcgacgcggc gcagcatgtt gcgcgtcatg aagaggccgc
     1441 cgacgacgcc gagcgccagc gtcaagagcg ccgcccagga gagcgtctcg gtaatgcgcg
     1501 agcggaagac tcgcgcatcg tggaggtcgc gcccgaccag cagcagatag ccgccgggga
     1561 gcgggaagac cgcggcgagc gcggtgtcgg aatcgtcgcc cgccgggctc ttcgagtgca
     1621 cgggaaagct gatgcgcgtc ggccgggcgg gtacggcgcc gggccagccc gagatgttgc
     1681 cggcgagcgg ctggagcgtc gcatcggtga tgaggtaaag cgaggtgtcg ctgtgctggc
     1741 cggcgacgcg ggcgcggatg atctcgacga ggccggcgag gccgcgctcg cgatactgct
     1801 cgcgcaagcc ggtgatctcg gcgtcgatcg tcacctcggt ctgctgctcg atgaaatcgg
     1861 cggtcgagaa atagatgaac accagcagcg ccgataccga gaccgcgaag agtccgaaat
     1921 agatggcggc gagcctgaag gcgttggtgc gcagcaggcg gaccatcacg agcccgcccg
     1981 caaggaatag ccggcgccgc ggatggtgtg cagcatcggc ttggcgaagc ctttgtcgat
     2041 cttctgccgc aagcggctca catgcacgtc gatgacgttg gtctgcggat cgaaatgata
     2101 gtcccacaca ttctcgagca gcatcgtgcg cgtcaccacc tggcccgcat ggcgcatgag
     2161 gaattcgagg agatggaact cgcgcggcaa aagctcgatg tcctgcccgc cgcgcgtgac
     2221 gttgcgcgtc aggaggtcca tctcgagatc gcccactttg agtttcgtcg gttgcgtcga
     2281 ggcgctgctg ccgcgtcgca gcaaggcctc gacacgcgcc aggagctcgg cgaaagcgaa
     2341 gggcttcgcg agatagtcgt cgcctccggc gcgcagcccc ttcacgcggt cgtcgacggc
     2401 gccgagcgcg ctcaggatca acacaggcgt gcgaatctcc gccgcgcgca gcgcggtgac
     2461 cagcgccagc ccgtccatcc ccggcagcat gcggtcgatg atcagcgcgt cgtagtttcc
     2521 ggcgctcgcc atgaacaggc cggtgcggcc gtcgggcgca aggtcgacca catatccgct
     2581 ctcggagagc cctttcgcga cataggccgc ggccgcgcgg tcatcctcga tgacgaggag
     2641 cttcatacct cggttcctgg ctcgaagcct tgtctttctg ctcgaacggg ctggtccgac
     2701 catagccagc cggttgacgg ggcgaaaggg tcctgcggtc gaggaacctc cgccccccgg
     2761 caaccgtttc cgtaatgcgc cttggtgctt ggagttaggg caacgcatga ccatgaacga
     2821 gctgaaggac gatgtgcctg cgcgggcgcg acgccagccg ccttcgatcc tcatcgtcga
     2881 ggacgaggca ctcgtggcaa gctacatccg cgaggtgctc gaggaatccg gcttcaccat
     2941 cgccggtatc gcttcctcgg ggcctgaagc catgtcgttg gccatagcga gccggcccga
     3001 tctcgcgctc gtcgatatca agctcgccgg ccccatggac ggcatcgagg tggccgagct
     3061 gatgcgctcg cggttcaata cgtattcgat cttcctgtcg ggtgtcgccg atcctcagac
     3121 catggaacgg gcccggaacg tcgaccattt gggcttcctg gagaagcctt tccggccgag
     3181 ccaagtcttc aacgcgctgc agcaggcact cgacaagctg ccctcgcact aggcttagac
     3241 gccggtctca gagcagtgtc accagcagcg cggtgagcca tcccagcgcc agcacgagcg
     3301 catcctgccg atcgagcttc cggtcttcca tggtccgtcc ctccaaaccg ggcgtaggat
     3361 agactgagaa caaacaaaga acaagagacg tcatcgagcg gcggccgggc tgggttaggc
     3421 gctctaccct aagtcgtatg cctttacgcc gccctcatga ttgagggtca tagtgcgggc
     3481 gcggcgggat ctcgcgccgc tttggcggtt gctcatgcgg ctctcgatgc gcctcatcct
     3541 cgcgatctct ttcgctgccc tctccgcggc cgccgtcgcc ggtcatttcg ttgtgctgcc
     3601 cgcgctcgag ttggcgacgt ccctgacggc gctcctactc ctcgccgcgc ttgcctacgc
     3661 gcttggtttg gagcggggca aacggcccgt cgcgggcttg gcggacgcca tgacgcgcca
     3721 aggcgtggaa ctggagcaca cccggagcgc ggctggcgtg accgccgcgt cgatgcgcca
     3781 tctcttcgac ggcaatccca taccgatgta tgtctacgat caggcgtcgt tgcgctttct
     3841 cgacgccaac gacagcgccg tcgcgcagta tggtcatagc cgcgagcgct tcctggcgat
     3901 gaccatcgag gacatccgtt gccgcgaaga ggtggcgcgg cttcgcgcgt cccgcgccgg
     3961 catgatatgg agcgagggct cggccggcat ctgggagcat cggaaggcgg acggcacgac
     4021 gatctcggtc gagatcatcc accgcagtat cctgttccaa gactgcgccg ccatactcgt
     4081 cgccgctatc gacgtgaccc acgcgtgtcg ggtcgcgcgc gagctgcgcg acagccagac
     4141 gcgcctcaag gcgctcgccg acaatctgcc gggcatcgtt tatcagtgtc ggctgaccgc
     4201 cggcggcgag atcagctatc ccttctgcag cgcggcggtc tctgacctgt tcgggcccgg
     4261 gcccgatggc gacgccgact ttgtcgtggt gacgcgctcc attctgccgg aggaccgcac
     4321 ggcgttcatg acgtcggtgc ggcgatccgc ggcggacctg tcgccctggt cgctcgattt
     4381 ccgctgccgc cgccgcaacg ggacgatctc gtggttcagt ggcaggggga agccgcggcg
     4441 tttcgccgat ggggagacgc tgtgggacag cgtgctcatc gacatctccg agcagaagaa
     4501 tgccgagcag gctttgcgcg agagcgaggc gcagcttcgc cgcagccgcg agcatctcgc
     4561 ccgggcgcag cgcaccgccg atatcggtag ctgggaaatc gatctgcgca gccgttcgct
     4621 cgagtggtcg gacgagctgt tccgcgtgct cggcctttcg cgcgagcagg tcgccccggg
     4681 attcgatcat atgatcgcgc gcttttgccc ggaagatcga gagcggatga gaggttggct
     4741 cgagctggtc tgcgcgggcg cgagcgtcgc ggccagcgag ggccggctcc ttctgcccga
     4801 cggcaaggag cgttgggtgc gcatggacgc
//
LOCUS       DASYNS010000594         4391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_3741375, whole genome shotgun sequence.
ACCESSION   DASYNS010000594 DASYNS010000000
VERSION     DASYNS010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3741375"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..906
                     /locus_tag="VGP50_18020"
     CDS             361..906
                     /locus_tag="VGP50_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019015621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV8029336.1"
                     /translation="MNLPNLISLGRLLSVPVAIWLVLSGELAGAFWLFVASGVSDAVD
                     GFIAKRFDQRTRLGALLDPLADKALLVSVYVTLGIGGYLPNWLVILVVFRDVLIVGGW
                     LLVAGLGQRMRWEPLYVSKLNTALQIGLIAVVLARLGLGLRDFGLVVVLTYGVGATTV
                     ISGAGYLVRWAGALAGVETDT"
     gene            903..1982
                     /locus_tag="VGP50_18025"
     CDS             903..1982
                     /locus_tag="VGP50_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV8029337.1"
                     /translation="MTTGERQQIFFWLGALVLIFALIYFLSPILLPFVAGSVIAYFLS
                     PVVSRLGRWGVRRSFGAAVVLLLFVLALLLIAALLLPLVQLQVAEFAHRVPVLVGEAR
                     ERVEQLTRLAEQKLSPDDVAKLRDMVASGASDLASWAARLVQGLLTSGIALANLLSLI
                     FITPIVAFFLLRDWDHIVALVDSWLPRQHLAVIREQARLVDATLSGFVRGQLLVCLGV
                     GIYYAVALTAIKLEFGLILGLLAGILTFIPYVGFATGFVLAMGLALVQFGDLSGLLWV
                     LGVFAVGQVLESNVLGPKLVGERVHLHPVWVIFALLAFGSVFGFLGVLLALPAAAVIG
                     VLVRFALSRYLASPLYDPANRLPPD"
     gene            1987..2646
                     /locus_tag="VGP50_18030"
     CDS             1987..2646
                     /locus_tag="VGP50_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HEV8029338.1"
                     /translation="MEGQLPLDLGHRPALGRADFLVAPSNAEAVAWLDRWPLWPAPAL
                     ALYGPKGSGKTHLAHVFAARAGARFIDPATLATERVPTLLGEARAAIVDDAAAAAAEP
                     LLHLYNVLAEQQGHLLLVAREPPAHWAIALPDLRSRLLACPAVALSPPDEALIGALLV
                     KLFADRQLVVGDDIVTYLTLRLERSFDAVLQAVATLDRAALAERRRITVPLARRVLGF
                     D"
     gene            complement(2652..2861)
                     /locus_tag="VGP50_18035"
     CDS             complement(2652..2861)
                     /locus_tag="VGP50_18035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029339.1"
                     /translation="MTIAIPFFGKSASRVARSVEAAPHQPVTRSPIPRLDPRRLRIFV
                     RPQPTLFQRCLATHMYFASRKSALD"
     gene            complement(3144..3674)
                     /locus_tag="VGP50_18040"
     CDS             complement(3144..3674)
                     /locus_tag="VGP50_18040"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV8029340.1"
                     /translation="MAGAVSSLYLLRHAKAVPQSEGTDRERALEPKGRRGAQAVAAWI
                     AEHRLSPELVLCSPSLRTRQTLDIVAPAFAHPPQILFEDGLYLATTRQLMARLKQIPV
                     SVKSAMAIGHNPGFQELAGILSDVATGPLPTRIAAGFPTGALAGYDVAVPWSALDRRR
                     AHLVTFVTPKELLRGE"
     gene            3737..>4391
                     /locus_tag="VGP50_18045"
     CDS             3737..>4391
                     /locus_tag="VGP50_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HEV8029341.1"
                     /translation="MAQALRAAASPIDLQTPARFINRELSWLAFNERVLAEADSPRHP
                     LLERLRFLSISANNLDEFYMVRVAGLKAQVLAGIATPSQDGLTPAQQLEAINGRAGKL
                     MRDQQVTWRVLVQQLAEAGISVIGPGDLAAEDRTWLEERFMAEIFPVLTPLAIDPAHP
                     FPFIANLGLGLALQLTRRGDGNRMQALLLMPSTLGRFVRLPGKNVRFLPLEQTIGLFL
                     "
BASE COUNT          570 a         1469 c         1485 g          867 t
ORIGIN      
        1 ctgctgtcgc tcgaccgtgg cggtgcgcgg ctcgagatcg cctatgccgg cgacgccgca
       61 cagctccgca cggcgctggc gcagcgcgac ctcgagctca gcggcagtga tcccgactgg
      121 gtgttgcggc gccgcctcgg cggcggctcg accgtgccgc gttaatgtgg ccgcggtgct
      181 agccgatatt cgcccgctct tccccgggag agaggatcga ggtggtggcg ccggtagagc
      241 agggcgcgac gctgtgcgcc tggccggcgg tgtcgagcga cgttctgctc gcgcgaagct
      301 gcggtcttgg cccgacactc ttctggaaga gagacggaga gcagcgcggg acgcccggcg
      361 atgaacctgc cgaatctcat ctcgctcggg cggcttcttt cggtgccggt cgcgatctgg
      421 ctcgtgctct cgggtgagct cgccggcgcg ttctggctgt tcgtggcatc cggcgtctcc
      481 gatgcggtcg acggcttcat tgccaagcgc ttcgatcagc ggacgcggct cggcgccttg
      541 ctcgatccgc tggccgacaa ggcccttctc gtgagcgtgt atgtgacact cggcatcggc
      601 gggtatctgc cgaactggct ggtcatcctc gtcgtgtttc gcgacgtgct gatcgtcggc
      661 ggttggcttc tggtcgccgg gctgggtcag cggatgcgtt gggagcccct ttatgtgagc
      721 aagctcaaca cggccttgca gatcgggctg attgccgtgg tgttggcacg gctcggcctt
      781 ggtctccgcg atttcggcct cgtcgtcgta ttgacctacg gcgtcggggc gacgacggtg
      841 atttcgggcg cgggctatct cgtgcgctgg gcaggcgcgc tggcaggggt ggagacggat
      901 acgtgacgac cggcgagcgg cagcagatct ttttctggtt gggcgcgctc gtcctgatct
      961 tcgcgctcat ctatttcctg agcccgattc tcctcccctt cgtggcgggc tcggtgatcg
     1021 cctatttcct cagccccgtg gtcagccggc tcggccgctg gggcgtgcgc cgctccttcg
     1081 gtgcagcggt cgtgctgctg ctcttcgtct tggccttgtt gctgatcgca gcacttcttt
     1141 tgccgctcgt gcagctccag gtcgcggagt ttgcccatcg cgtcccggtg ctcgtcggcg
     1201 aggcgcgcga gcgcgtcgag cagcttacgc gcctcgccga gcagaaactc tcgcccgatg
     1261 acgtcgccaa gttgcgggac atggtggcgt cgggcgcgtc ggatctcgcg agctgggccg
     1321 cgcggctggt gcagggtctc ctgaccagcg gcatcgcgct cgccaatctg ctgtcgctga
     1381 tcttcatcac gccgatcgtc gcgttctttc tgctgcgcga ctgggaccac atcgtcgcgc
     1441 tggtcgatag ctggctgccg cgccagcatc tcgccgtgat ccgcgagcag gcgcgcctag
     1501 tcgacgccac gctctcgggc tttgttcgcg gacagctgct cgtctgtctc ggcgtcggca
     1561 tctattacgc ggtggcgctc accgccatca agctcgagtt cggtctcatc ctcggcttgc
     1621 tggcgggcat tctgaccttc attccctatg tcggctttgc gaccggcttc gtcctggcaa
     1681 tgggcttggc gctggtgcag ttcggcgatc tgtcggggct cctctgggtg ctgggcgtct
     1741 tcgcggtggg gcaggtgctc gagtccaatg tgctggggcc caagctcgtg ggcgagcgtg
     1801 tccatcttca tccggtttgg gtcatcttcg cgttgctcgc ctttggcagc gtcttcggct
     1861 tcctcggcgt gctgctggcg ctgcccgctg cggccgtcat cggtgtactc gtgcgctttg
     1921 ccctgagccg ctatctcgca agcccgctct acgaccccgc gaaccgcttg ccgcccgatt
     1981 gacgcgatgg aggggcagct tccgctcgat ctcggccacc gcccggcgct cgggcgcgcc
     2041 gattttctag tagcgccgtc caatgcggag gctgtcgcgt ggctcgaccg ctggccgctc
     2101 tggccagcgc cggcgctcgc gctctatggg cccaagggca gcggcaagac ccatctcgct
     2161 catgtattcg cggcgcgcgc cggggcgagg ttcatcgatc ccgcgacgct ggcgacggag
     2221 cgtgtgccga cgcttctcgg cgaagccaga gcggcgatcg tggacgatgc cgccgccgcc
     2281 gccgcggagc cgctgcttca tctctacaac gtcttggccg agcagcaagg ccacctgctg
     2341 ctggtggcgc gcgagccgcc ggcgcattgg gcgatcgcgc tgccggacct ccgctcgcgc
     2401 cttctcgcct gcccggcggt cgccctgtcg ccccccgatg aggcgctcat cggtgctctt
     2461 ctcgtcaagc tcttcgccga tcggcaactc gtggtcggcg acgatatcgt gacgtatctg
     2521 acgctgcggc tcgagcgctc gttcgacgcc gtgcttcaag cggtggcaac gctcgaccgg
     2581 gcggcgttag ccgagcggcg gcgcatcacc gtgccgctcg cgcgacgtgt gctgggcttc
     2641 gactgagctt ctcagtcgag cgcgctcttg cgtgaggcga agtacatatg ggtcgcgagg
     2701 cagcgctgga agagcgtcgg ttggggccga acgaagatgc gcaagcggcg cggatcgagc
     2761 cgtggtatcg gagaccgcgt gaccggctgg tgcggcgctg cctcgacgga gcgggcgacg
     2821 cgactcgcgc tcttgccaaa gaacggtatc gcaatggtca tggatgcgct ccctacgggc
     2881 cctgatgctt cgacgcacgc tatcgatgat tcgcccgagc agcatgtgag cccggtcaca
     2941 ctgggtgttc ggtggtccgg ccccgtcccc aactgagctc tctcccctgt gctgacgtcg
     3001 agcggctccc cattgcgaac gaatcgtcca tgtcgacgct tgacgagcaa cgatcgccct
     3061 cgcgactcga cgtcgatccg gacatgcctc tttcgtaggc gcttcgcgac ggcgatgaga
     3121 tccgaggcat cgatcgggtc ttgctactcg ccccgtagca gctctttcgg cgtgacaaag
     3181 gtgacgagat gagcgcggcg gcggtcgagc gcggaccagg gcacggccac gtcatagccc
     3241 gccagcgccc ctgtcgggaa tccagcggcg attcgcgtcg gcagcggccc tgtcgccaca
     3301 tccgataaaa tgccggccaa ttcctggaac ccgggattgt ggccgatcgc catggcactc
     3361 ttcacgctga ccgggatctg cttgagccgc gccatgagct ggcgcgtcgt ggcgaggtag
     3421 agcccgtcct cgaacaggat ctgcggggga tgggcaaagg ccggggcgac gatgtccaga
     3481 gtctggcgcg tgcgcagcga gggggagcac agcaccagct ccggcgagag ccgatgctcg
     3541 gcgatccagg ccgcaaccgc ctgggcgccg cgccggccct tcggctcgag cgcccgctcg
     3601 cggtccgtgc cttcgctttg aggtaccgcc ttggcgtgcc tcaacaggta aaggctgctc
     3661 acggcgccgg ccatgacttt gccatggtcc cgttataaac tgccgctctc gtgcagggaa
     3721 tggggagtgc agaaacgtgg cccaggcttt acgcgccgcg gcatcgccga tcgaccttca
     3781 gacgccggcg cgtttcatta accgtgagct ttcctggctc gccttcaacg agcgggtcct
     3841 cgccgaagcg gacagcccgc gccatccgct gctggagcgg ctgcgcttcc tgtcgatctc
     3901 ggccaacaat ctcgacgagt tctacatggt gcgcgtcgcc gggctcaagg cgcaggtgct
     3961 ggccggcatt gccacgccca gccaggacgg cctgacgccg gcgcagcagc tcgaggccat
     4021 caatggccgc gccggcaagc tcatgcggga ccagcaagtg acgtggcgtg tcctggtgca
     4081 gcagctcgcg gaggccggca tttccgtcat cgggcccggc gacctcgcgg ccgaagatcg
     4141 cacctggctc gaagagcgct tcatggccga gatcttcccg gtgctgaccc cgcttgccat
     4201 cgatcccgcg catccttttc ccttcatcgc caatctcggc ctcggccttg cgctgcagct
     4261 cacacggcgc ggcgacggca accgcatgca ggcgttactg ctgatgccaa gcacgctcgg
     4321 ccgtttcgtc cggctgccgg gcaagaacgt gcgcttcctg ccgctcgagc agacgatcgg
     4381 gcttttcctc g
//
LOCUS       DASYNS010000595        10505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_2025247, whole genome shotgun sequence.
ACCESSION   DASYNS010000595 DASYNS010000000
VERSION     DASYNS010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2025247"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VGP50_18050"
     CDS             complement(<1..704)
                     /locus_tag="VGP50_18050"
                     /inference="COORDINATES: protein motif:HMM:NF012531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029342.1"
                     /translation="MVNSAGLPRQGLYDPRNEHDACGVGFIAHIKNRRSHGLVADGIR
                     ILLNLAHRGAVAADPLAGDGAGILVQLPDKLFRAEAKRLGFALPPSGEYGVGMMFLPR
                     NAETRQACEEALALFIKTEGQSVLGWRDVPVDSSVLGESIKPIEPVIRQIFIARGPNC
                     PDVDAFERKLFVIRKQTHLYIRAQGLPDQATFYIASLSARTIIFKGMVIPERLALYYS
                     DLGDKRLESALALVHAR"
     gene            complement(892..1680)
                     /locus_tag="VGP50_18055"
     CDS             complement(892..1680)
                     /locus_tag="VGP50_18055"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8029343.1"
                     /translation="MSRLRGKRAIVTGAGSGIGRASAKLFAAEGAAVLVVDKVEQAVA
                     ETAAQIAAAGGTAIAMTADVGSESSVRTYIDKAIKELGGLDVVYANAGISGGLVPLFE
                     QTAEMWQEILRVNLISPFLAIKYAAAYMRQQGKGSIICTASVAALRANAGGSPYSASK
                     AGVVSLVQTAANALTGTGVRVNAICPGLIETGMTRPIFEGARARGTEGKIGQINPLQR
                     AGQPEEIAAMALFLASDEASYVNGQAFAVDGGLSSTHPFAQQRR"
     gene            complement(1761..3560)
                     /locus_tag="VGP50_18060"
     CDS             complement(1761..3560)
                     /locus_tag="VGP50_18060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV8029344.1"
                     /translation="MPVTAIQIYDEMDRHHSREAELNAEALRLATLVDMEQNRVLEGA
                     RQLLIAVAQLRSLREHDRTRCKDLFGRFATRFPYYVYIAALDLKGNAFCATRTDLDRG
                     ALTPERPFFAGALTSHDFAVGGFSRTKDGKGVLQLGFPIINERDGRVTGVLVAALNLE
                     WLSANLGMNILPPHAVLNVADRYGTIIAHLPQSSALNVIGEPLPETRRALLYSPSAGT
                     VESYDATGHSRIFGYDPIIVPPQGLYIEVGLDRDGAFAEIARGTVRHTMAVAAALLVG
                     FTLSWLGAHYFIRRPTAALVRAARRWRHGDWTARVGAGESNSDFARLGHAFDQMAEAL
                     SEREGELIRAKEYAEAANRSKSSFLANMSHELRTPLNAIIGFSEVIVGQIYGAGAGER
                     YRECASYINASGQHLLRLVNDILDLSKLAAGQLDLAESLVDLKTLMRDSVDLVLSQAQ
                     QKGVTISTAIAEGLPPLMAGELRLKQVLLNLLSNAVKFSRPGGAVALSVELRPSGDLA
                     IAVADQGIGMKPQDIPIALEPFRQIDNALSRSYEGTGLGLPLAKMLVEKHGGSLALES
                     ALGIGTTVTVVLPASRLRPAQRTIARQRALLAP"
     gene            3544..4587
                     /locus_tag="VGP50_18065"
     CDS             3544..4587
                     /locus_tag="VGP50_18065"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV8029345.1"
                     /translation="MAVTGTKAIATRRNSRPRIPMPESLCAHAFRGQHGLQSLTLGKV
                     LRSWERAEMGAIESLGVIGVGTMGEPICVNLARKCGLPVYAVDRRPEPLRRLAADGVA
                     PCASLAELASRTDLVFLSLPSGREVEALCLGAGGIAAIRGRIRLVVDCGTSPVALTRR
                     IAARLAAVGIDFADAPVARTREAAQQGRLSIMVGASPELFERVSPYLLAFGTDITHCG
                     PVGSGQAVKILNNMVLFETVGALAEALAIGRRAGLDERLLLEALSKGSADSFALRNHG
                     MKAMLPRDFPENAFAADYALKDLSYALELARDGGVAVAGAELAGARLAEAIRRGHGRR
                     YWPVLLKVVAGEE"
     gene            4593..5018
                     /gene="arfB"
                     /locus_tag="VGP50_18070"
     CDS             4593..5018
                     /gene="arfB"
                     /locus_tag="VGP50_18070"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEV8029346.1"
                     /translation="MIRITPEIVIDEREIEEKYIRSGGPGGQNVNKVATAVQLRFDAA
                     ASRALDEALRRRLATLAGRRMTKDGVIVITAERFRTRERNRADALARLVDLLQRAAIR
                     PKRRRATRPTAGSRERRLAGKAERSRTKRLRQTGPDFSS"
     gene            5096..6265
                     /locus_tag="VGP50_18075"
     CDS             5096..6265
                     /locus_tag="VGP50_18075"
                     /inference="COORDINATES: protein motif:HMM:NF004811.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEV8029347.1"
                     /translation="MARKAAIVGVADTLLRDGVLAEDGTVLQIQARAAKAALEEAGLS
                     FDDVDALFAAGAWGIPGPGLFMTTTLAEYLGLRLSYMDGTQIGGSSFESHLSHAVAAI
                     AEGRCEVALILYGSRQRSDASRTLGGRGGAMLNSQFDAPFGLPTPVGAYAMAAMRHMH
                     VNGTTAEQLAEIAVATRKWAALNPAATMRDPLGIAEVLASPMIADPLHLRDCCLVTDG
                     GGAIVVTSLERARDLKKPPVVVAGAAEATSHWAISSMPDLAWLQHAADSGKRAMAMAG
                     VGPGDIDVVEIYDSFTITVLMSLEALGFCGRGEGGAFVQGQRTAPGGDFPLNTNGGGL
                     SYCHPGMYGLFLITEATRQLRGECGSRQVKDAEVALAHGTGGVLSTVASCILTRT"
     gene            6270..6674
                     /locus_tag="VGP50_18080"
     CDS             6270..6674
                     /locus_tag="VGP50_18080"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEV8029348.1"
                     /translation="MTYEKPLPVIDGESQPFWQAAREHRLAIMRCRDCGKHFFYPREL
                     CPFCHRAAVEWANASGRGKIYTFTIARRPAGPAFKADAPYVVALVELEEGPRLMTNIV
                     TKEVDRIRIGQAVKAVFDDVTTEVTLVKFAPA"
     gene            6717..7235
                     /locus_tag="VGP50_18085"
     CDS             6717..7235
                     /locus_tag="VGP50_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV8029349.1"
                     /translation="MTDLIRATRRDLLLAPLLATLPLALGGESTAAAVDPAMTIIKLP
                     PELTWTKNEGFPEKSVEAAPLWGKTGDPGLYYVLIRWYPGYMSAPHWYETDRYSLVVS
                     GTWWVGSGDKFDLESTVPAPAGSLVRRIARTPHYDGVKAGATEPAVIAICGMGPITFH
                     NTDPATPGWRKV"
     gene            complement(7237..8238)
                     /locus_tag="VGP50_18090"
     CDS             complement(7237..8238)
                     /locus_tag="VGP50_18090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV8029350.1"
                     /translation="MNIAIISDAWRPQVNGVVRTLTALIGELGARGYKVAPITPDLFR
                     TFPCPTYPEIRLAVCPRRHVGELIEAADPDAIHISTEGPLGIAARRHCLTRGYPFTTA
                     FHTRFPEYVAARFAVPLPWSYALMRRFHAPSGGVMVATETVRRELAARGFHNLRRWNR
                     GVDAALYEPSGGGALPDLPRPIFLTVGRIAVEKNFDAFLALDLPGSKVVVGDGPMLDK
                     LKRRYPEAHFLGRREGKDLARLYASADAFVFPSRTDTFGLVLLEALASGVPVAAYPVP
                     GPLDVIGDSGAGVLDEDLRRAALAALEIPREHCRAHALRFTWPASADQFVANLCPLR"
     gene            complement(8235..9050)
                     /locus_tag="VGP50_18095"
     CDS             complement(8235..9050)
                     /locus_tag="VGP50_18095"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008495138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEV8029351.1"
                     /translation="MTAPSYYRTIWISDIHLGTRGCKAEFLVDFLRDTESETLYLVGD
                     IVDGWRLRRSWYWAQSHNDVIQKVLRKARKGTKVIYIPGNHDEWLRDYTQLTLGGVLL
                     LDEAIHETADGRKLLVLHGDAFDGVVRYAKWLALLGDWAYTVALHLNHHFNLIRRRFG
                     YPYWSLSAYLKSRVKNAVEFVSNYADVIVEEARKRGVHGVVCGHIHQAEMRDIRGVLY
                     CNDGDWVESCTALVEHFDGRLEILNWTSLRALDPLAAAPAGRRELAPATADAP"
     gene            complement(9162..9527)
                     /locus_tag="VGP50_18100"
     CDS             complement(9162..9527)
                     /locus_tag="VGP50_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029352.1"
                     /translation="MQLLPPERRLRNAVKHGWTRLVGAARRRRRLALRQRPPRRAPVM
                     AGIDPVALTVLAVNRLGPATLRTVLTGKPLTVRVPNARVGEIFRAALAEMQKTRLTDR
                     LIAIAIDDDPDAGRVPTRA"
     gene            complement(9673..>10505)
                     /locus_tag="VGP50_18105"
     CDS             complement(9673..>10505)
                     /locus_tag="VGP50_18105"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV8029353.1"
                     /translation="PFTLKGTGTYVVGHGRVAVIDPGPALADHVSALLQALRGETVTH
                     ILVTHTHLDHSPAAAALKQATGAPTYGFGPHGSGRAEDATGVGGVTEEGGDHAFHPDH
                     RLREGDSVAGPGWHLAAVHTPGHTSNHLCFALAEERALFSGDHVMGWSTSVIAPPDGD
                     MAAYMRSLEKLLHRDDAVYWPTHGPPIPDPKPFVQAFIDHRHARSDAILQRLAAGDET
                     IEGIVDHVYLGLDPRLKGAAARSVLAHLVELVERGRVVADGPPTLSTRYRAIRRENRA
                     "
BASE COUNT         1692 a         3405 c         3681 g         1727 t
ORIGIN      
        1 cgcgcatgca ccagcgccag cgccgattca agccgcttgt cgccgagatc gctgtagtag
       61 agcgcgagcc gctccgggat gaccatgccc ttgaagatga tcgtgcgtgc cgagaggctc
      121 gcgatataga acgtcgcctg gtcggggagg ccctgcgcgc ggatgtagag atgcgtctgc
      181 ttgcgaatga cgaagagctt ccgctcgaag gcgtcgacat ccggacagtt gggtccgcgg
      241 gcgatgaaga tctggcggat gaccggctcg atcggcttga tgctttcgcc gagcacgctg
      301 ctgtcgaccg gcacgtcgcg ccagcccaat acgctctggc cttcggtctt gatgaagagc
      361 gcgagggctt cctcgcaggc ctgccgggtc tcggcgtttc gcggcaggaa catcatgccg
      421 acgccatatt cgcccgaagg cggcagggca aagcccaggc gcttcgcctc ggcccggaac
      481 agcttgtcgg ggagctgaac gagaatgccg gcgccatctc ccgccagcgg gtcggcagcg
      541 acggcgccgc gatgggcgag gttcagcagg atgcgaatgc catcggcaac cagaccgtga
      601 ctccggcggt tcttgatatg cgcgataaaa ccgacgccgc acgcatcgtg ctcgttgcgt
      661 gggtcgtaca gtccttgccg cggcagtcct gcggagttaa ccattcgccc ccttcggtcg
      721 atcgcacgac cttaaatcaa ggtccggaac ccaaggtcac tggcggcgga caggggcaga
      781 cccggcatgc ctacgccatg gcgatcgaaa agagcatcgc ggggcgaccc cctgcgagtc
      841 aagcgcgctg ccgcgtcccg cgcccagcga aacggctcct ctgccggtct cctagcgccg
      901 ttgctgagcg aaggggtgcg tgctggagag gccgccatcc acagcgaagg cctggccgtt
      961 cacataggat gcttcatcgc tggcgaggaa gagggccatg gcagcgatct cttcgggctg
     1021 gcccgctcgc tgcagtgggt tgatctggcc gatcttgccc tccgtgcccc gcgcccgcgc
     1081 cccctcgaag atcggccgcg tcatccccgt ctcgatgagg ccggggcaga tggcgttgac
     1141 gcggacgccg gtcccggtga gcgcattggc ggccgtctgt accaggctga ctacgccggc
     1201 tttgctggcg ctgtaaggac tacccccggc attggcacgc agtgccgcca ccgaggcggt
     1261 gcagatgatc gatcccttgc cctgctgccg catgtaggcg gcggcatatt tgatcgccag
     1321 gaacgggctg atcaggttta cgcgcaggat ctcctgccac atctccgccg tctgctcgaa
     1381 gagcggcacc aggccgccgc tgatcccggc attggcatag acgacgtcga gcccgccgag
     1441 ctcctttatg gccttgtcga tataggtacg gacggagctt tcgctgccga catcggcggt
     1501 catggcaatt gccgtgccgc cggccgccgc gatttgcgcg gcggtctcgg cgacggcttg
     1561 ctcgaccttg tcgacgacca gaaccgcggc cccttccgcg gcgaaaagct tggcgctcgc
     1621 gcgcccgatc ccgctgcccg cgcccgtcac gatggcgcgc ttgccccgaa gccggctcat
     1681 tcccgccctc cccagacatc gcaaccgatg cggggcggat gatgctcttc gaacagaaaa
     1741 ttgcaatcgc gtgacgttgg ctagggtgcc agcagagcgc gttggcgcgc gatggtgcgc
     1801 tgcgccggcc gcagccgcga cgccggcaat accaccgtca ccgtggttcc gatgccgagg
     1861 gcgctctcga gcgccaggct gccgccatgc ttctcgacga gcatcttggc gagcggcagt
     1921 ccaaggcccg taccttcata agagcgtgag agcgcgttgt cgatctggcg gaagggctcg
     1981 agggcgatcg ggatatcctg tggcttcatg ccgatgccct ggtcggccac ggcaatggcg
     2041 agatcgcccg agggcctcaa ttccacggag agcgcgactg cgccgccggg gcgtgagaac
     2101 ttgaccgcgt tcgacagcag attgagcagc acctgcttca gccgcagctc ccccgccatc
     2161 aggggcggca gcccctcggc gatcgcggtc gagatggtta cacccttctg ctgcgcttgg
     2221 ctcagcacga ggtcgacgct gtcgcgcatg agcgtcttga ggtcgaccag ggactccgcc
     2281 aggtccaatt gtccggcggc aagcttcgag agatcgagga tgtcgttgac gaggcgcagg
     2341 agatgctggc ccgacgcatt gatgtagctg gcgcattcgc ggtagcgctc gcccgcgccg
     2401 gcaccataga tctggccgac gatgacttcc gagaaaccga tgatggcgtt gagcggcgtg
     2461 cgcagctcat ggctcatatt cgcgaggaag ctggacttcg agcggttggc cgcttcggcg
     2521 tattcctttg cccggatcag ctcgccctcg cgttcgctca gcgcctcggc catctggtcg
     2581 aaagcgtgac cgaggcgcgc gaaatccgaa ttgctctctc cagcgccgac gcgcgccgtc
     2641 caatcgccgt ggcgccatcg ccgggcggca cgtaccagcg cggcggtcgg ccgccggatg
     2701 aagtagtgtg cgccgagcca tgacagcgtg aagccaacga gaagcgccgc cgccaccgcc
     2761 atggtgtgcc gcaccgtgcc gcgggcgatc tcggcgaagg cgccgtcgcg atcgagcccg
     2821 acctcgatat aaaggccctg gggcggcacg atgatcggat cgtagccgaa gatgcgcgaa
     2881 tggccggtgg cgtcgtagct ctcgacagtg ccggcgctgg ggctgtagag gagggcgcgc
     2941 cgcgtttcgg gcagcggctc gccaatgacg ttgagcgcac tgctctgcgg cagatgcgcg
     3001 atgatcgtgc catagcggtc ggcgacgttc agcacggcgt gcggcggcag gatgttcatg
     3061 ccgagattgg cgctcagcca ttcgagattg agcgcagcca ccagcacgcc cgtcacgcgt
     3121 ccgtcgcgct cgttgatgat cggaaagccg agctgcagca cacccttgcc gtctttcgtg
     3181 cgcgagaagc cgccgacggc gaaatcgtgg ctggtgagcg cccccgcgaa gaagggtcgt
     3241 tccggtgtca gcgcgccgcg gtcgagatct gtgcgcgtgg cgcagaacgc attgcccttg
     3301 aggtcgaggg cggcgatgta gacgtaatag ggaaaccggg tcgcgaagcg gccgaacagg
     3361 tccttgcacc tcgtgcgatc atgctcgcgc aagctgcgca gctgggcgac ggcgatgagc
     3421 agctggcgcg cgccttcgag aacgcggttc tgctccatgt cgacgagcgt cgcgagcctg
     3481 agcgcttcag cgttcagctc cgcttcccgg gaatggtgac ggtccatctc gtcatagatc
     3541 tggatggccg tcaccggcac caaggcaatc gccacaagga ggaatagccg cccgaggata
     3601 cccatgccgg agtcgctttg cgcccacgct ttccgcggcc agcatgggct acaatcgtta
     3661 actctcggta aggttcttcg aagctgggaa agggcggaaa tgggggcgat cgaaagcctg
     3721 ggcgtgatcg gcgtcggcac catgggcgag ccgatctgtg tcaacttggc gaggaaatgc
     3781 gggcttccag tgtacgcggt ggaccggcgc cccgagccgt tgcggcgtct cgcggcggac
     3841 ggcgttgccc cctgcgcctc gctggcggag ctcgcgagcc ggaccgacct ggtcttcctc
     3901 tctctgccga gcgggcgcga ggtcgaggcg ctgtgccttg gcgcgggcgg tattgccgct
     3961 attcgaggcc gaatccggct ggtcgtcgac tgcggtacat ctccggtggc gctcacccgc
     4021 aggatcgcgg cgcgtcttgc cgccgtcggc atcgactttg ccgatgcgcc ggtggcgcgc
     4081 acgcgcgagg ccgcgcagca gggacgcctc agcatcatgg tgggcgcgag ccccgagctg
     4141 ttcgagcgcg tctcgcccta tctgcttgcc ttcggcaccg acatcaccca ttgcggtccc
     4201 gtggggagcg gccaggctgt caagatcctc aacaacatgg tgctgttcga gacggtgggc
     4261 gcgctagccg aggcgcttgc catcggccgc cgggccggcc tcgacgagcg gctgttgctg
     4321 gaggcgctga gcaagggctc cgccgacagc ttcgcgctgc gcaatcacgg catgaaggcg
     4381 atgctgcctc gcgatttccc tgagaatgcc ttcgctgctg actatgcgct caaggatctg
     4441 tcctacgcgc tcgagctcgc gcgcgacggt ggcgttgccg tcgccggggc cgagctcgcg
     4501 ggtgcgcgcc tcgccgaagc gatccggcgc ggccatggcc ggcgctactg gccggtgctg
     4561 ctcaaggtcg tggccggcga ggaatgatcg cgatgatcag gatcacaccg gagatcgtta
     4621 tcgacgagcg cgagatcgag gagaagtaca tccgctcggg cggacccggt gggcagaacg
     4681 taaacaaggt ggcgactgcg gtgcagctgc gcttcgacgc cgccgcttcg cgcgcgctcg
     4741 atgaagcgct ccgccgtcgt ctcgccacgc tggccgggcg gcgcatgacc aaggacggcg
     4801 tgatcgtcat cacggccgag cggtttcgca cgcgcgagcg caatcgcgcg gacgccttgg
     4861 cgcgtcttgt cgatctcctc cagcgcgccg ccatccgccc caagaggcgg cgcgcgacac
     4921 ggccgaccgc cggctcgcgc gagcgccggc ttgccggcaa ggcggagcgg tcccgcacca
     4981 agcggctgcg ccagacaggc cccgatttct cgtcttgaga cgtcggcgtt cgccgcaagg
     5041 ccgccccgcg tgcttgccgg ttgccactcc agacgcggcg gtgtagacgt cgcccatggc
     5101 gcggaaagcg gcaatcgtcg gcgtggccga tactctcttg cgtgatggcg tcctcgccga
     5161 ggatggtacc gtccttcaga tccaggcgcg ggcggcgaaa gcagccctcg aggaagcggg
     5221 tctatccttc gacgacgtcg atgccctctt tgctgctggc gcctggggca ttcccggtcc
     5281 cgggctcttc atgacgacga cgctggccga gtatctgggc ttgcgccttt cctacatgga
     5341 cggcacgcag atcggtggct cgtctttcga gtcgcatctc agccatgcgg tggccgcgat
     5401 cgccgaaggg cgatgcgagg tggcgctgat cctctatggc agccggcagc gcagcgacgc
     5461 gtcgcgcacc ttgggcggcc gcggcggggc gatgctcaac agccagttcg atgcgccttt
     5521 cgggttgccc acgcccgtcg gcgcttacgc catggcggcg atgcggcaca tgcatgtcaa
     5581 cggcaccacg gccgagcagc tcgccgagat cgccgtggca acgcggaaat gggcggcgct
     5641 caatcccgcc gccacgatgc gcgatccgct cggcatcgcc gaggtgctgg cgagcccgat
     5701 gatcgccgat cccctgcatc tcagggattg ctgcctcgtc accgatggcg gcggggcgat
     5761 cgtcgtcacc agcctcgagc gggcgcgcga cttgaaaaag ccgccggtgg tcgtcgccgg
     5821 cgccgccgag gcgacgagcc actgggccat ttcctcgatg ccggatctgg cctggctgca
     5881 gcatgccgcc gacagcggca aacgggcgat ggcgatggcg ggcgtgggtc ctggcgacat
     5941 cgatgtcgtc gagatctatg actccttcac cattaccgtc ctcatgtcgc tcgaggcgct
     6001 gggcttttgc gggcgcggcg agggcggcgc cttcgttcag ggccagcgca cggcgcccgg
     6061 tggcgatttc ccgctcaaca ccaatggcgg cgggctctct tattgccatc ccggcatgta
     6121 tggcctcttc ttgatcaccg aggcgacgcg ccagctgcgc ggcgaatgcg gctcacgcca
     6181 ggtcaaggac gccgaggtgg ccctcgccca tggcaccggc ggcgtgttgt cgacggtggc
     6241 gagctgtatc ctgacgcgga cgtgagacga tgacctacga gaagccgctt cccgtgatcg
     6301 atggcgagag ccagcctttc tggcaggcgg cgcgcgagca tcgtctcgcc atcatgcgtt
     6361 gccgcgattg cggcaagcac ttcttctatc cgcgcgagct ttgccccttt tgccatcggg
     6421 ccgccgtcga gtgggcgaat gcgagcggcc gcggcaagat ctataccttc accatcgccc
     6481 gccgccccgc gggccccgcc ttcaaagccg acgcgcctta tgtcgtggcg ctggtcgagc
     6541 tcgaagaagg gccgcggctc atgaccaaca tcgtcaccaa agaggtcgat cggatccgca
     6601 tcggccaggc ggtgaaggcc gtcttcgacg acgtgaccac ggaagtgacg ctcgtgaaat
     6661 tcgcgcccgc gtaatttcgc ggcgcgacca accgctccat tcgcgaggct tgcgacatga
     6721 ccgatctcat tcgagcgacg cgacgcgact tgctcctcgc gccgctgctg gcgactttac
     6781 ctctggcgct cggcggcgag agcaccgcgg cggcggtcga tccggcgatg accatcatca
     6841 agctgccgcc ggagctcacc tggacgaaaa acgaaggctt tcccgagaag agcgtggaag
     6901 ctgcgccgct ctggggcaag acgggtgacc ctggcttgta ctatgtgctg atacgctggt
     6961 atcccggcta tatgagcgcg ccccattggt acgagacgga ccgctatagc ctcgtcgtct
     7021 ccggcacctg gtgggtcggc agcggcgaca aattcgatct cgagagcacc gtgcccgcac
     7081 ccgccggcag cctcgtccgg cgcatcgcac gcacgccgca ttatgacggc gtgaaagcgg
     7141 gcgcgaccga gcccgcggtc atcgccatct gcggtatggg cccgatcaca ttccacaata
     7201 ccgacccggc cacgcccggg tggcgcaagg tttaggctag cgcagcgggc agagattcgc
     7261 gacgaactga tcggccgagg caggccaggt gaagcgcagc gcatgggcgc ggcagtgctc
     7321 gcgcggaatc tcaagcgcgg cgagcgccgc gcggcgcaga tcctcgtcga gcacgcccgc
     7381 gccgctgtcg ccgatgacgt cgagcggacc cggcacggga taggcggcga cgggaacgcc
     7441 cgaggcgagc gcctcgagca gcaccaggcc gaaggtgtcg gtgcggctcg gaaacacgaa
     7501 agcatcggcc gaggcgtaga gccgcgccag atccttgccc tcgcggcggc cgaggaaatg
     7561 cgcctcggga tagcggcgct tcagcttgtc gagcatcggg ccgtcgccca ccacgacctt
     7621 gctgccgggc agatcgagcg cgaggaaggc gtcgaaattc ttctcgacgg caatccgccc
     7681 gacggtgagg aagatcggcc gcggcaggtc cggcagcgca ccgcctcctg aaggttcgta
     7741 gagcgcggcg tcgacgccgc ggttccagcg ccgcagattg tggaagccgc gtgcggcgag
     7801 ctcgcgccgc accgtctccg tcgccaccat gacgccgcct gagggcgcgt ggaaacgccg
     7861 catcagcgcg tagctccagg gcagcggcac ggcaaagcgc gccgcgacat attcggggaa
     7921 gcgcgtgtgg aaggcggtgg taaagggata gccgcgggtg aggcaatggc ggcgggcggc
     7981 gatgccgagc gggccttcgg tgctgatgtg aatggcgtcg ggatcggccg cctcgatgag
     8041 ctcgccgacg tgacgccgcg ggcagacggc gaggcggatc tcgggataag tggggcacgg
     8101 gaaggtgcgg aagagatcgg gtgtgatcgg cgccactttg tagccgcgcg cgccgagctc
     8161 gccgatgagc gccgtcagcg tgcggacgac gccgttcact tgcggccgcc acgcgtcgct
     8221 gatgatggcg atgttcaagg ggcgtcggcc gtcgcggggg cgagctcgcg gcgccccgcg
     8281 ggcgcggcgg cgagcggatc gagtgcccgc agcgatgtcc agttcaggat ctcgagccgg
     8341 ccgtcgaaat gctcgacgag agcggtgcag ctctcgaccc agtcgccgtc gttgcaatag
     8401 aggacgccgc ggatgtcgcg catctcggcc tggtggatgt ggccgcagac gacgccgtgg
     8461 acgccgcgct tgcgcgcctc ttcgacgatc acgtcggcgt agttgctgac gaactcgacc
     8521 gcgttcttga cgcggctctt gagataggcg gagagcgacc agtagggata gccgaagcgg
     8581 cggcggatga gattgaaatg gtggttgagg tggagcgcga cggtgtaggc ccagtcgccc
     8641 aggagcgcca gccatttcgc atagcgcacg acgccgtcga aggcgtcgcc atgcaggacg
     8701 aggagcttcc gcccgtcggc ggtctcgtgg atcgcctcgt cgagcaggag gacgccgccc
     8761 agcgtcagct gcgtataatc gcgcagccat tcgtcgtgat tgccggggat gtagatgacc
     8821 ttggtgccct tgcgcgcttt gcgcagcacc ttctggatga cgtcgttgtg gctctgcgcc
     8881 cagtaccagg agcggcggag ccgccagcca tcgacgatgt cgccgacgag gtagagcgtc
     8941 tccgattccg tatcgcgcag gaaatcgacg agaaactcgg ctttgcagcc gcgcgtgccg
     9001 agatggatgt cggagatcca gatggtgcga taatacgaag gtgccgtcat gcgcttgcct
     9061 cgcggcgata ccgcgcgagg tcgtcagcga tcccggcctc gcgcaaatga agcagaaaga
     9121 tatgtcgatc gccggtgccc cggccgtgcc gcgcagccca ctcaggcgcg ggttggaacg
     9181 cgtcccgcat caggatcgtc gtcgatggca atggcgatga gccggtcggt gaggcgcgtc
     9241 ttctgcatct cggcgagcgc cgcgcggaag atctcgccga cccgcgcgtt gggcacccgc
     9301 acggtgagcg gcttgccggt gaggaccgtg cgcagcgtcg ccgggcccag gcgattgacc
     9361 gcgagcacgg tgagcgcgac cggatcgatc cccgccatca cgggggcgcg gcgcgggggg
     9421 cgctgccgga gcgccaggcg gcgccgccgg cgcgccgcgc cgacgagccg cgtccagcca
     9481 tgcttgacgg cgttgcgcag gcgacgctcc ggaggcagaa gctgcatgac gcatgtgcta
     9541 tcaccgtttg gtgacagttt ttggtcgggg catcgccgtg tatgacggtt ttttcggaaa
     9601 tcgctgaaaa ttttagatat ttccgggtct tgccgacgac tcttgcggct aaaagaccgg
     9661 acttcgcccc tcttatgcgc gattttcgcg gcgaatcgcg cgataacggg tcgagagcgt
     9721 cggcgggccg tcggcaacca cccggccgcg ctcgaccagc tcgacgagat gggcgagaac
     9781 cgagcgtgcg gccgcgcctt tgaggcgcgg gtcgaggccg agatagacgt ggtcgacgat
     9841 cccctcgatg gtctcgtcgc ccgcggcgag ccgctgcagg atggcgtcgc tccgcgcatg
     9901 gcgatgatcg atgaaggcct gaacgaaagg cttggggtcg gggatcggcg gaccatgggt
     9961 cggccagtag acggcgtcgt cgcggtggag gagcttctcg agcgagcgca tataggcggc
    10021 catgtcgcca tcgggcggcg cgatcacgct ggtcgaccag cccatcacgt gatcgcccga
    10081 gaagagcgcg cgctcttcag ccagcgcgaa gcagagatgg ttcgaggtgt gccccggcgt
    10141 gtgaaccgcg gcgagatgcc agcccggccc cgcgacgctg tcgccctcgc gcagccgatg
    10201 atcggggtgg aaggcgtggt cgccgccttc ctcggtgacg ccgccgacgc cggtcgcgtc
    10261 ttcggcgcgg cccgagccat ggggaccgaa gccataggtc ggagcgcccg tcgcttgctt
    10321 gagcgccgcg gccgccggcg aatggtcgag atgcgtatgc gtcaccagga tatgcgtgac
    10381 ggtctcgccg cggagcgcct gaagcagggc cgacacgtga tcggcgaggg cgggcccggg
    10441 atcgatgacg gcgacgcggc catgccccac cacataagtg ccggtgcctt ttaaggtgaa
    10501 ggggc
//
LOCUS       DASYNS010000596         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_5457021, whole genome shotgun sequence.
ACCESSION   DASYNS010000596 DASYNS010000000
VERSION     DASYNS010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5457021"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VGP50_18110"
     CDS             <1..454
                     /locus_tag="VGP50_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV8029354.1"
                     /translation="LQAIEEGDLSPENVKRIANELGVSEPDVVSMNRRLAAPDHSLNA
                     PLKADGEGEWQDWLVDESSDQETTLGERQELGLRRDLLRGALSHLTERERDILMERRL
                     KEQPTTLEDLSQKYGISRERVRQIEVRAFEKLQKAIKTAAFDRRLLPS"
     gene            complement(414..929)
                     /locus_tag="VGP50_18115"
     CDS             complement(414..929)
                     /locus_tag="VGP50_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029355.1"
                     /translation="MARRRPTSARKGAAGRGGGRATLVLVVFFLVILAALAPPVCVLL
                     AAGLLPSLAALILDRHPALYLTRAVGAMNLAGITWPALALWDASVSIAGLREVLENPL
                     SWLSMYGGASLGWLVYLAIPPVALIWIEAQADDTRVRLAARAKALVEEWGEEVTGRAN
                     SATAGGRTPPS"
     gene            complement(933..>2959)
                     /locus_tag="VGP50_18120"
     CDS             complement(933..>2959)
                     /locus_tag="VGP50_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEV8029356.1"
                     /translation="FFLKETRPVAVREPQTEAAKSRALLGGGFEIYPDRPCPEFASPG
                     STAFVASHRDRPAARLFAVIGDPDWPPRSEMMTALSQLAAPALTLPLAWGVVDWAPAG
                     RRSFATVFEQPPGQRLVTSLAQRIEPAGEDELMGVILPPLVAGLRDLWGAHLTHRAIR
                     PTNLFRDAAGGGALRLGECVTAPPALAQPVVCEPLESGLAMAVGRGHGTAADDIYALG
                     VTVLFLLLGQLPAAALGDDQILAEKIARGSYVALLGGARIPAAMTELLRGTLADDPRE
                     RWSAPDIALWLNGRRALPRQQAQPKRAPHPFVFLDKNYHTGRALARAFAADPMAALAV
                     LRGPDFATWIRHSLAEEECSKALAAALADDRGPEERLVGRVCIALDPAAPVRYKGTAT
                     TLDGFAVAALAGLRGRVAVQPIADAIIVGLPQFWCALRRESTQEQAAIQKNFDRLRVL
                     LEDRRPGFGMERIVYELNPQLHCLSSAVEADYVLDPGDLLPALERASERRRDEDLQID
                     RHLAAFIAARFRLAGNDWHDGLAGVDPTDRALGALYLFGRLQSRYGPPALPALTQRLG
                     RQLPALIERFHNRARRARLRAALPRSLAKGSLADVLAFLDSVPERIRDHQGFNQAKRD
                     YAAIERDLDLLGGGAARQPERAAELGARYAAVTAKALAWLVALVMLVTRL"
BASE COUNT          530 a          995 c         1064 g          370 t
ORIGIN      
        1 gctgcaggcg atcgaggaag gcgatctctc gcccgagaac gtcaagcgca tcgccaacga
       61 gctgggtgtg tcggagccgg acgtggtgag catgaaccgc cgccttgccg ctcccgacca
      121 ttccctcaac gcgccgctca aggccgacgg cgaaggcgag tggcaggatt ggctggtcga
      181 tgaatcgagc gaccaggaaa cgaccctcgg cgagcgccag gagttgggcc tgcggcgaga
      241 cctgctgcgc ggcgcgctgt cgcatctcac cgagcgcgag cgcgacatcc tcatggaacg
      301 gcggctcaag gagcagccga cgacactcga ggacctgtca caaaaatacg gcatatcgcg
      361 cgagcgcgtc cgccagatcg aggtgcgtgc tttcgagaag ctccagaagg cgatcaagac
      421 ggcggcgttc gaccgccggc tgttgccgag ttagcgcggc cggtcacttc ctcgccccat
      481 tcttccacca gcgccttggc gcgcgcggca agccgcaccc gcgtatcgtc ggcctgcgcc
      541 tcgatccaga tcaaggcaac tggcgggatc gcgaggtaga cgagccagcc gaggctcgct
      601 ccgccataca tcgagagcca gctcagagga ttctccagca cctcgcgaag cccggcgatc
      661 gagacgctgg cgtcccagag cgcaagagcg ggccaggtga tgcccgccag attcatggcg
      721 ccgacggcgc gcgtcaagta aagcgcgggg tggcgatcga ggatcagcgc cgcgaggctc
      781 gggagcagcc ccgccgcaag caagacgcag acgggcggcg ccagcgccgc caggatcacg
      841 agaaagaaca cgacgagcac gagcgtcgcg cgaccgccgc cgcggccagc ggcacctttc
      901 cgtgcggagg tgggacggcg tcgcgccatg actcagagcc tcgtcacgag catgaccaag
      961 gcgacgagcc aggcgagcgc cttggcggtc accgcggcat aacgcgcgcc gagctccgcg
     1021 gcgcgctccg gctgccgcgc cgcgccgccg cccaagagat cgagatcccg ctcgatcgcc
     1081 gcatagtcgc gctttgcctg gttgaagcct tgatgatcgc gaatgcgctc aggcacgctg
     1141 tcgagaaaag ccaggacatc cgcgaggctg cctttggcca gcgatcgcgg cagcgcggcg
     1201 cgcagccgcg cccgccgcgc ccgattgtgg aaccgctcga tcagggccgg gagctgccgt
     1261 cccagacgct gcgtcagcgc cggcagcgct ggcgggccat agcgcgactg cagtcgtccg
     1321 aagagataaa gggcgccgag cgcgcgatcg gtcgggtcaa cgccggcaag tccgtcatgc
     1381 cagtcattgc cggccaggcg aaagcgcgcg gcgatgaagg cggcgaggtg acgatcgatc
     1441 tgcaaatcct cgtcgcgacg ccgctccgaa gcgcgctcga gcgccggaag caagtcgccg
     1501 ggatcgagga cgtaatcggc ctcgaccgcg gacgagaggc agtggagctg cggattgagc
     1561 tcatagacga tgcgctccat gccgaagccg ggacggcgat cctcgagaag gacgcgcagg
     1621 cgatcgaaat tcttctggat cgccgcctgc tcttgcgtcg attcgcggcg cagcgcgcac
     1681 cagaattgcg gcaggccgac gatgatggca tcggcaatcg gctggacggc aacacgcccg
     1741 cgcaagccgg cgagagccgc aacggcaaag ccatcgagcg tcgtggcggt gcccttgtag
     1801 cgcaccggcg ctgcgggatc gagcgcgatg cagacgcggc cgaccaggcg ctcctcggga
     1861 ccgcgatcgt cggcgagggc cgcggcgagc gccttcgagc attcctcctc ggcgagactg
     1921 tggcgtatcc aggttgcgaa atccggcccg cgcaagacgg ccagcgccgc catgggatcg
     1981 gccgcgaagg cgcgggcgag ggcgcggccg gtgtgatagt tcttgtcgag aaaaacgaag
     2041 ggatgcggcg cccgctttgg ctgcgcctgc tgcctcggca aggcgcggcg gccgttgagc
     2101 cagagggcga tgtccggcgc cgaccagcgc tcgcgcgggt cgtcggcgag cgtgccgcgg
     2161 agcagctccg tcatcgccgc tggaatgcgc gccccgccga gcagcgcgac ataagagccg
     2221 cgcgcgatct tctccgcgag gatctggtca tcgccgagag cggccgcggg gagttgcccc
     2281 agcagaagaa agaggaccgt gacgccgagc gcatagatgt catcggccgc cgtgccgtgg
     2341 ccgcgaccca ccgccatggc caagccgctt tcgagaggct cgcagacgac gggctgcgcg
     2401 agggcgggcg gtgctgtcac gcattcgccg agcctgagcg cgccgccacc ggccgcgtcg
     2461 cgaaagaggt tcgtcggacg gatcgcgcga tgcgtcaggt gggcgcccca gaggtcgcgc
     2521 aagcccgcga cgagcggggg caaaatgacg cccatcagct cgtcttcgcc cgcgggctcg
     2581 atccgctgcg ccaaggaggt gacgaggcgc tggcccggcg gctgctcgaa caccgtagcg
     2641 aagctccggc gcccggccgg agcccagtcg acgacgcccc aggcaagcgg cagcgtgagc
     2701 gccggcgcgg cgagttggct caaggcggtc atcatctccg agcggggcgg ccaatcggga
     2761 tcgccgatga cggcgaagag acgcgcggcg ggccggtcgc gatgactcgc aacaaaggcc
     2821 gtgctgcccg gcgaggcgaa ctccggacag ggacgatcgg ggtagatctc gaagcccccg
     2881 ccgagaagcg cccgcgattt cgcggcctcg gtctggggct cgcgcacggc cacgggcctg
     2941 gtctccttca agaaaaacc
//
LOCUS       DASYNS010000597         4962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_779965, whole genome shotgun sequence.
ACCESSION   DASYNS010000597 DASYNS010000000
VERSION     DASYNS010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779965"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..944
                     /locus_tag="VGP50_18125"
     CDS             201..944
                     /locus_tag="VGP50_18125"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV8029357.1"
                     /translation="MSPRPLLIDAGRRLALLAGVLAVVAVTLAVTGGLAWRGVGVALI
                     AYGLITAGVVAGLGYHPYRRFGAANVVTLTRAAYAALLVGILGDGTASLSETGRWALV
                     AAGAAALALDGVDGWLARRSHLASVFGARFDMETDALSVLALSALVYRSGQVGAWVLT
                     AGLMRYIFVLAGHLWPPLAAPLPPSQRRKVVCVILIVGMLTALAPPVGTATAQGLCLV
                     GLVLLVYSFGADCVRLAAASRENRAPAPG"
     gene            984..2114
                     /gene="ribA"
                     /locus_tag="VGP50_18130"
     CDS             984..2114
                     /gene="ribA"
                     /locus_tag="VGP50_18130"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014238953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEV8029358.1"
                     /translation="MLNRRPAVAPLRDARLLRQIDGAVTELRRGGFVALREPELILLV
                     QAAEALSGESLARLRSFSSDPVSLVLTRRRAAALGLVEPPREPGGAVLLRVTADASAV
                     ALRDLADPAATAVPPLPLETAVLAAGAADAAVELMKLARLLPAAVVARLPAHGAASVV
                     EREHLLLLDAADVPTYQATAARSLRPVSEARVPLVDAEDTRIIAFRPSDGGIEHLAIL
                     IGEPKQDSAVLVRLHSECFTGDLLGSLRCDCGDQLRGAIAEIARAGSGVLLYLAQEGR
                     GIGLVNKLRAYQLQDDGFDTVDANEQLGFDADERGYLPAAEMLRQLGFDRVRLLTNNP
                     EKVAALIRCGIAVVERVPHIFPANGHNERYLRTKATRSGHWL"
     gene            2367..3740
                     /locus_tag="VGP50_18135"
     CDS             2367..3740
                     /locus_tag="VGP50_18135"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEV8029359.1"
                     /translation="MASIAGKTVAGLVGLGVTCIAAAALAASPDAPPDSSPAPSAVAA
                     PEDWSAHFQLTVVGQGYPGFPSTVQGADSLPAHGQIRETISGTAFFGGRLPWSGGELY
                     FDPEFNQGFGLARTLGVDGFVNGEAQKAGFDTPKPNVARLFLRQTFGLGGAQETLEPD
                     ANQLGETVDIARVTVTAGKMAVPDIFDDNQYAHDPRTTFLNWSLWESAAWDYPADQKG
                     YTDGLALELNEKDWALRGGWFLEPKIANERDLDPRFWKHYGAVVELETRHEWFGEPGK
                     LRYLVFANRADMGNLQQAVDLAVATGTPADIAAVRHDSWKAGAALNLEQSLVGDLGLF
                     SRLSWNDGHTEGWAFTDIDRSAVLGLSLKGASWSRPDDTVGLADAVNGLSKAHQLFFA
                     NGGTGILAGDGSLNYAPEEIVEAYYNARVLEKLWLAFDYQFVANPAFNQDRGPVHVFA
                     LRAHVEF"
     gene            4095..4373
                     /locus_tag="VGP50_18140"
     CDS             4095..4373
                     /locus_tag="VGP50_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007423520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HEV8029360.1"
                     /translation="MSHTIHEKRKLLNRVRRIRGQVEAVERALEDEAGCARVMHLIAG
                     CRGAINGLLAEVVEDHVRTHLVDPVKDPTALNAEAADQLLEVVHSYFK"
     gene            4384..>4962
                     /gene="dmeF"
                     /locus_tag="VGP50_18145"
     CDS             4384..>4962
                     /gene="dmeF"
                     /locus_tag="VGP50_18145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008494262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HEV8029361.1"
                     /translation="MMGGEAALPAIRHSHVFLGAGHERNARRTWGVIWLCSAMMAAEI
                     IGGGLFGSLALIADGLHMSTHAGALLLAALAYSYARRHAEDTRFVFGTGKIGDLAGFS
                     SAIVLAMIALLIGYEAVARLLHPVAIDFNEAIPIAVVGLGVNIASVWLLGGDDHDHGH
                     GAHHERDGEDDDDDHDHDHEHHTEDHAGDGHGA"
BASE COUNT          737 a         1683 c         1660 g          882 t
ORIGIN      
        1 tcaaatcagc catagcattc gatcacgcgt atttgaatcg catttgcgac attccacacc
       61 cttgagtgac gcatcctgct cctctccggc actggtttgt ggcttgtgat tgttgcgatt
      121 tcgggtcatt gtctgctccg gcgtccaccc gtccctggcg aggccggcgc cgcaaaaacc
      181 ttatccgagg actttccacc ttgtcgccgc gccctctgct gattgatgcc ggaaggcggt
      241 tggcgctgct tgccggtgtg ctcgccgtcg tggcggtgac gctcgcggtg acgggcgggc
      301 tcgcctggcg cggcgtcggg gtggcgctca tcgcttatgg gctcatcacg gccggcgtcg
      361 tggccggcct cggttatcat ccctatcgcc gcttcggggc ggccaatgtc gtaactctga
      421 cacgcgccgc ctatgcagcg ctgctcgtcg gcattctcgg cgacggcacg gcgagcttga
      481 gcgagaccgg ccgctgggct ctggttgcgg cgggcgctgc cgctttggcg ctcgatggcg
      541 tcgacggctg gcttgcccgg cgcagccatc tcgccagcgt cttcggcgcg cgcttcgaca
      601 tggagaccga cgcgctctcc gtgctggcgc tgtcggcgct cgtctatcgc tccggccagg
      661 tcggggcatg ggtgctgacg gccggtctca tgcgttatat cttcgtcctg gcgggacacc
      721 tgtggccgcc gctggcagcg ccgctgccgc cgagccagcg ccgcaaggtg gtttgcgtca
      781 tcctgatcgt cggcatgctg acagctttgg cgccgccggt cggaacggcg acagctcagg
      841 gactgtgctt ggtgggcctt gtgctcttgg tctattcctt tggcgccgat tgcgttcgtc
      901 tggccgccgc gtctcgcgag aacagggcgc cggcgccagg atgaggggat gaggcgaatg
      961 atcgactgcg ccggagaaat cccgtgctga accgcaggcc ggcggtggcg ccgcttcgcg
     1021 atgcgcgtct cctgcgccag atcgatggcg cggtgacgga gctccggcgc ggcggtttcg
     1081 tcgcgttgcg cgagcccgag ctgatcctgc tcgtgcaggc cgccgaagcc ctgagcggcg
     1141 agagcctcgc ccgcttgcgg agtttctcga gcgatcccgt ctcgctggtg ctgacgcgtc
     1201 ggcgcgcggc ggcccttggt ctcgtcgagc cgccgcgcga gcccggcggc gccgtgctgc
     1261 tgcgcgtcac cgccgatgcc tcggcggtcg cgttgcgcga cctcgccgat cctgccgcca
     1321 cggcggttcc gccgctcccg ctcgagaccg cggtgctggc agccggcgcc gccgatgccg
     1381 cggtcgagct catgaagctg gcgcgtctct tgccggcggc cgtggtcgcg cgtctccccg
     1441 cgcacggcgc cgcgtccgtc gtcgagcgcg agcatctgct gctgctcgac gccgcggacg
     1501 tcccgaccta tcaggcaacg gcggcgcggt cgctgcgccc ggtgagcgag gcgcgcgtcc
     1561 cgctcgtcga tgcggaggac acgcgcatca tcgccttccg cccgagcgat ggcggcatcg
     1621 agcatctcgc cattctgatc ggcgagccca agcaggacag cgccgtgctg gtacgcctcc
     1681 attccgaatg cttcaccggc gacctgctcg gcagtctgcg ctgcgactgc ggcgaccagc
     1741 tgcgcggcgc catcgccgag atcgcccgcg ccggcagcgg cgtgctgctc tatctggcgc
     1801 aggaaggccg cggcatcggc ctcgtcaaca agctgcgcgc ctatcaactg caggacgacg
     1861 gcttcgacac cgtcgatgcc aatgagcagt tgggattcga cgccgatgaa cggggctatc
     1921 tcccggccgc cgagatgctg cggcaattgg gtttcgaccg cgtgcggctc ctcaccaaca
     1981 atcccgagaa agtggcggcc ttgatccgtt gcggcatcgc cgtcgtcgag cgcgtgccgc
     2041 atatattccc ggcgaacggc cacaacgagc gctatctgcg caccaaggcg acgcgcagcg
     2101 gccactggct ttgacgacgt cgatgccccg agtcttggaa ctgcaacact agacccggcg
     2161 ctgccccgct atctttacac ttcccgttga cacgcgtcgg cgcgccgaca tattcctgcg
     2221 gcgcgccgtt ggcggccgcc tgcagcgcac agcgctaaca gcaatgacga tgggcgacgg
     2281 ttcgacggac gcggagagcg gctttgcgtc gagcgatgcc gccgacttgc ccgacttcgg
     2341 aagagccgcc gctcggggga gcgtcgatgg cgtcaatcgc agggaagacg gtcgccggcc
     2401 ttgtcggcct gggcgtgacc tgcatcgccg ctgcggcgct cgcggcgagc cccgatgcgc
     2461 cgcctgattc ttctcccgcg ccgagcgccg tcgccgcgcc cgaggattgg agcgcccatt
     2521 tccaattgac ggtggtcggc cagggctatc ccggctttcc ctcgaccgtg cagggtgcgg
     2581 acagccttcc ggcgcatggg caaattcgcg agacgatcag cggcacggcc tttttcggcg
     2641 gccgtcttcc ctggagcggc ggcgagctct acttcgaccc ggaattcaac cagggcttcg
     2701 gcctcgcgcg caccttgggt gtcgacggct tcgtcaacgg cgaagcgcag aaggccggct
     2761 tcgacacgcc gaagcccaac gtcgcccgct tgttcctgcg ccagaccttc gggctgggcg
     2821 gcgcgcagga gacgctcgag cccgatgcca accagctcgg cgagaccgtc gacatcgcgc
     2881 gcgtcaccgt caccgccggc aagatggccg tccccgacat cttcgacgac aaccagtacg
     2941 cccatgatcc gcggaccaca ttcctcaact ggagcctctg ggaatcggcg gcctgggact
     3001 atccggccga tcagaagggc tacaccgatg gcctggcgct cgagctcaac gagaaggact
     3061 gggcgcttcg cggcggctgg ttcctcgagc caaaaatcgc caatgagcgc gatctcgatc
     3121 cgcgcttctg gaagcattat ggcgccgtgg tcgagctcga gacccgtcat gagtggttcg
     3181 gcgagcccgg caagctgcgc tacctcgtct tcgccaatcg cgccgacatg ggcaatctgc
     3241 agcaggcggt cgatctggcg gtggcgacgg gcacgcccgc cgacatcgcc gctgtgcgcc
     3301 acgacagctg gaaggctggc gcggcgctca atctcgagca gagccttgtc ggggatctcg
     3361 ggcttttctc gcggctgagc tggaatgacg gccataccga gggctgggct ttcaccgaca
     3421 tcgaccgcag cgcggttctg gggttgagcc tcaaaggggc gtcctggagc cggcccgacg
     3481 acacggtcgg cctggcagac gcggtcaacg ggctctcgaa agcgcaccag ctgttcttcg
     3541 ccaatggcgg gaccggcatc ctcgccggcg acggcagctt gaactacgcg cccgaggaga
     3601 tcgtcgaggc ctattacaac gcgcgcgtcc tcgagaagct gtggctcgcc ttcgactatc
     3661 agttcgtcgc caatcccgcg ttcaaccagg accgcggccc ggtgcacgtc ttcgccctgc
     3721 gggcgcacgt cgaattctga cccgcgccgc attcatcacg tgagttggtc gaggagcgag
     3781 ccctttgtta gcgatcgagg aacgcagagg gcgcggtggt tacgcagagg tcgccgaggg
     3841 tggctattcc tccgcgacct ccgcggagcc tccgcggcct ccgcgttcat cttcttgttt
     3901 gttttggtgc gaccgccggt gacggggcct ttggtcatca ttcgcaagag ccgctgatcg
     3961 tccttgttta aggcgcgtcc cttgcggctg cggctaagag gaagtgctgc aacacgtgaa
     4021 aacctcaacg aaaatcgttg acgtaaattg atggcaggga tagggtaccc ccctatccta
     4081 tcggggcggc cgcgatgagc cacaccatcc acgaaaagcg gaaactgctc aatcgcgtgc
     4141 gccgcatccg cggccaggtc gaggccgtcg agcgcgcgct cgaggacgag gccggctgcg
     4201 cccgggtcat gcatctcatc gccggctgcc gcggcgcgat caacgggctg ctggcggaag
     4261 tcgtcgagga tcacgtccgc acgcatctcg tcgatcccgt caaggatccg accgcgctca
     4321 atgccgaagc ggcggaccag ttgctggagg tcgtccacag ctacttcaag tgaggaggca
     4381 ccgatgatgg gcggtgaggc cgcgctgccg gcaatccggc acagccatgt gttcctcggt
     4441 gccggtcacg agcgcaacgc gcgtcggacc tggggcgtga tctggctgtg cagcgcgatg
     4501 atggccgccg agatcatcgg cggcggcctc ttcggctcgc tggcactcat cgccgacggg
     4561 ttgcacatgt cgacccatgc cggcgcgctg ctgctggcgg cactcgccta cagctacgcc
     4621 cgccgccacg cggaggacac gcgcttcgtc ttcggcacgg gaaagatcgg cgacctcgcc
     4681 ggattcagca gcgccatcgt gctggcgatg atcgcgctgc tcatcggcta tgaggcggtc
     4741 gcgcgcctgc ttcatcccgt cgccatcgat ttcaacgagg cgatcccgat tgccgtggtc
     4801 ggcctcggcg tgaacatcgc cagcgtctgg ctcctcggcg gcgacgatca tgatcacggg
     4861 cacggcgctc atcacgagcg cgatggggaa gatgacgacg acgaccatga tcatgatcac
     4921 gagcatcata ccgaagatca cgccggcgac ggccacgggg ca
//
LOCUS       DASYNS010000598         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_1560086, whole genome shotgun sequence.
ACCESSION   DASYNS010000598 DASYNS010000000
VERSION     DASYNS010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560086"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..353)
                     /locus_tag="VGP50_18150"
     CDS             complement(207..353)
                     /locus_tag="VGP50_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029362.1"
                     /translation="MNESANLPDDSASDRLRRRVDEAFADLRPNVPAADVFKRLREQD
                     ANRK"
     gene            complement(441..1265)
                     /locus_tag="VGP50_18155"
     CDS             complement(441..1265)
                     /locus_tag="VGP50_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV8029363.1"
                     /translation="MADISYRALFDLSGKTALITGACGILGRRFAAGLAEFGAALALV
                     DLDADATTAFAAEIAHRYRGRAIGIACDIADPAAVAGMAERVERELGGAEILMNNAAT
                     KGADIDAFFAPVEHFDLATWRQVMSVNLDGMFLVAQAIGSAMAERGAGSIIQTASIYG
                     LLAPDQRIYEGSEYNGRPINTPAVYTASKAGVIGLTRHLATYWGPRGVRVNTLTPGGV
                     SSGQNNTFSQRYSARIPLGRMAEADDLVGAAVFLASDASRYVTGQNLVVDGGLSAW"
     gene            complement(1272..1988)
                     /locus_tag="VGP50_18160"
     CDS             complement(1272..1988)
                     /locus_tag="VGP50_18160"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEV8029364.1"
                     /translation="MKRLCTICARAGSKGVRSKNTRPLAGKPLIAHSLEQARASGLFD
                     AIAVSSDSDEILAIARHFGATLLVKRPDALASDTAAKLPAIQHALLDCERRLGTRFDV
                     LVDLDATSPLRLPEDIRAAVALLERAPGANVITGAPARRSPYFNLVETRPDGTVCLSK
                     PPACAIVRRQDAPPCYDMNASIYAWWTEDFRRAPAVFTPSTRLYTMPEERSLDIDSEL
                     DFRIVDFLMSARAAPAAAAE"
     gene            complement(1985..2902)
                     /locus_tag="VGP50_18165"
     CDS             complement(1985..2902)
                     /locus_tag="VGP50_18165"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV8029365.1"
                     /translation="MTLSALVVGLGSIGQRHLRLLRALGLDVAAVSRRQGAAGRCYAS
                     IGEALGDTNPGYVVIANETAAHRPALVALADAGYSGTVLVEKPLFAAPAPLPANRFAG
                     LYVGYNLRFHPLLRAVRQLLAEEPAISAQIYVGQYLPEWRPGRDYRDTASAGRAAGGG
                     VLRDLSHELDYMTWLLGPCRRVAALGGHLGPLAIETDDVQALLLSFARCPVATAQLNY
                     LDREGARDIVINTASRTIRADFLRGTLRVNGEETRFAVARDETYLAQHRCALGNERAL
                     LCSAAEGLAVTDLIAAAERAADGGAWVAL"
     gene            complement(2899..>3678)
                     /locus_tag="VGP50_18170"
     CDS             complement(2899..>3678)
                     /locus_tag="VGP50_18170"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEV8029366.1"
                     /translation="QVPLLDDAGRVAGLELLGDVTLRSERDNWVVLMAGGLGQRLMPL
                     TEDCPKPLLRIGGQPILEIILKSFARQGFQRFFLSVNYKAEMVRAHFGDGSRFGVQIE
                     YLQEDRKLGTAGALGLLTERPKHPLLVMNGDVLSGINFASLLDFHVEQRATATMAVRE
                     HTVEVPFGVIDLAADDYLASVTEKPRYSWFVNAGIYVLEPHVLHLLERGETIDMPTLL
                     DRVLANGERIAAFPVREYWRDIGRLEDLEGASAEDPAAFSA"
BASE COUNT          592 a         1136 c         1397 g          553 t
ORIGIN      
        1 gtccctccca ccaggaacat tcgaccccac catcgtcctt aaggtcggtc acggtcattg
       61 aaggtccgcc cgaggggagg cgggccaagt cacccggctt gaacgccatg tggtatattt
      121 tgggcagaaa tcacggttgc acaagaaggg tatggatttt gcggctttta tgggcaaagc
      181 cccttgcggg gaaggggttt cgtcggtcac ttgcggttgg cgtcttgctc gcgaaggcgt
      241 ttgaagacgt cggcggcggg gacattcggc cggaggtcgg cgaaagcttc gtcgacccgc
      301 cggcgtagac ggtcgcttgc ggagtcgtcg ggcaggttgg cggactcgtt catcgggatt
      361 cccctcaccc tccctccgct gcgcggcggg cccctccctc tccgacaagc ggagagggat
      421 cttagctcct tcctgctata tcaccaggcg ctgaggccgc cgtcgacgac gaggttctgg
      481 ccggtgacgt agcgggaggc gtcggaggcg aggaacacgg cggcgccgac gaggtcgtcg
      541 gcttcggcca tgcgacccaa ggggatgcgg gcggaatagc gctggctgaa cgtattgttc
      601 tggccgctcg agacgccgcc cggcgtcaaa gtgttcacgc gcacgccgcg agggccccaa
      661 tatgtcgcga gatggcgcgt caggccgatg acgccggctt tcgacgctgt gtagacggcg
      721 ggcgtgttga tggggcggcc gttgtattcg gagccctcgt agatgcgctg gtcgggggcg
      781 aggaggccgt agatcgaggc ggtctggatg atgctccccg cgccgcgctc ggccatggcc
      841 gagccgatcg cctgcgccac caggaacatg ccgtcgaggt tcaccgacat cacctggcgc
      901 caggtggcga gatcgaagtg ctcgaccggg gcaaagaaag cgtcgatgtc ggcgcccttg
      961 gtggcggcgt tgttcatcag gatctcggcg ccgccgagct cgcgctcgac acgctcggcc
     1021 atgccggcga cggcagcggg atcggcgatg tcgcaggcga tgccgatcgc gcgcccgcga
     1081 tagcgatgcg cgatctccgc agcgaaggcg gtcgtcgcgt cggcgtcgag gtcgacgagg
     1141 gcgagcgccg cgccgaactc ggcgagcccc gcggcgaagc ggcggccgag aatgccgcag
     1201 gcgccggtga tgagggcggt cttgccggaa agatcgaaga gcgcgcgata ggagatgtcg
     1261 gccatgcgtg gttactccgc agcggcggcg ggcgcggcgc gcgcgctcat caggaaatcg
     1321 acgatgcgga aatcgagctc cgaatcgatg tcgagcgagc gctcctcggg catcgtatag
     1381 agacgcgtcg acggcgtgaa cacggccggc gcgcggcgga agtcttccgt ccaccaggca
     1441 tagatcgacg cattcatgtc gtagcagggc ggcgcatcct ggcggcggac gatggcgcac
     1501 gctggcggct tgctgaggca gacggtgccg tcgggccgcg tttccaccag attgaaataa
     1561 ggcgagcggc gcgccggcgc gccggtgatg acgttggcgc cgggcgcgcg ctcgagcaag
     1621 gcgacggcgg cgcggatgtc ttcaggcagc cgcaacggcg aggtggcgtc gaggtcgacc
     1681 agcacgtcga aacgcgtgcc gagccggcgc tcgcagtcga ggagagcgtg ctggatcgcc
     1741 ggcagcttgg cggccgtgtc gctggcgagc gcgtcgggac gtttcaccag cagcgtggcg
     1801 ccgaagtgtc gcgcgatcgc caggatctcg tcgctgtcgc tgctgacggc gatggcgtcg
     1861 aagaggccgc tcgcgcgcgc ctgctccagc gaatgggcga tgagcggctt gccggcgagc
     1921 ggccgggtgt tcttggagcg cacgcccttg gagccggcgc gcgcgcagat ggtgcagagc
     1981 cgcttcatag cgccacccag gcgccgccgt ccgcagcgcg ctcggcggcg gcgatcagat
     2041 cggtcacggc gaggccctcg gcggcgctgc acaaaagggc gcgctcgttg ccgagggcgc
     2101 agcgatgctg ggccagatac gtctcgtcgc gcgcgacggc gaagcgcgtc tcctcgccat
     2161 tgacgcgcag cgtgccgcgc aggaagtcgg cgcggatggt gcggctcgcc gtgttgatga
     2221 cgatgtcgcg tgcgccctcg cgatcgagat agttgagctg cgccgtggcg acggggcagc
     2281 gcgcgaagga gaggaggagg gcctgcacgt cgtcggtctc gatggcgagg ggaccgagat
     2341 ggccgccgag cgcggcgacg cgccggcagg gaccgaggag ccaggtcatg tagtcgagct
     2401 catgggagag atcgcgcagc acgccgccgc ctgccgcgcg gccggccgac gcggtgtcgc
     2461 gataatctct gccgggacgc cactccggca gatactggcc gacatagatc tgggcgctga
     2521 tcgccggctc ctctgcgagc agctgccgca cggcgcgcag cagcggatgg aagcggagat
     2581 tgtagccgac atagaggccg gcgaagcggt tggccggaag cggtgccggt gcggcgaaaa
     2641 gcggcttctc gacgagcacc gtgccgctgt agccggcgtc ggccaaggcg acgagtgcgg
     2701 ggcgatgcgc cgcagtctcg ttggcgatca cgacatagcc gggattggtg tcgccgagag
     2761 cctcgccgat cgaggcgtag cagcgcccgg ccgcgccctg gcgccggctg acggcagcga
     2821 cgtcgaggcc gagcgcgcgc aagagccgga gatgcctctg gccgatcgag ccgaggccga
     2881 cgacgagcgc cgacagagtc atgccgagaa ggccgcggga tcttcggcgc tggcgccttc
     2941 gagatcctcg aggcggccga tgtcgcgcca gtattcgcgc acgggaaagg cggcgatgcg
     3001 ctcgccgttg gcgagcacgc ggtcgagcag tgtcggcatg tcgatggtct cgccgcgctc
     3061 caggagatgg agcacatgcg gctcgaggac atagatgccg gcattgacga accaggaata
     3121 gcgcggcttc tcggtgacgc tggcgaggta atcgtcggcg gcgaggtcga tgacgccgaa
     3181 gggcacctcg accgtgtgct cgcgcacggc catggtcgcg gtcgctcgct gctcgacatg
     3241 gaagtcgagg aggcttgcga aattgatgcc gctcaagacg tcgccattca tgacgaggag
     3301 cgggtgcttc gggcgctccg tcagcaggcc gagcgcgccg gccgtgccga gcttccggtc
     3361 ctcctggaga tattcgatct ggacaccgaa gcgcgagccg tcgccgaaat gcgcgcgcac
     3421 catctcggcc ttgtagttga ccgagaggaa gaagcgctgg aagccctggc gggcgaagct
     3481 cttgaggata atctcgagga tcggctggcc gccgatgcgc agcagcggct tggggcagtc
     3541 ctcggtgagg ggcatgaggc gctggccgag gccgcccgcc atcagcacca cccaattgtc
     3601 gcgctcgctg cgcagcgtga cgtcgccgag cagctcgagc ccggcgacgc ggccggcgtc
     3661 gtcgagaagc gggacctg
//
LOCUS       DASYNS010000599         3733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_466037, whole genome shotgun sequence.
ACCESSION   DASYNS010000599 DASYNS010000000
VERSION     DASYNS010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466037"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VGP50_18175"
     CDS             complement(<1..798)
                     /locus_tag="VGP50_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEV8029367.1"
                     /translation="MDQGRPYFLCGIGGSGMLPLALILRARGVSVAGSDRALDQGRTA
                     AKFDFLRAQGIALFPQDGSGITGPDLVVVTSAAVEAQVPDVQQALAIGAKLVTRAALL
                     ADLFNAAPRSIGVAGTSGKSTTTAMIGWILQRDGQDPTVMNGAVMKNFIAPDLPFASA
                     LIGKSGLFVSEVDESDGSIAQYAPHIAVLNNIALDHKSLDELRLLFRDFVERSAVAVL
                     NLDNGETAALAKLRPPEKVISYSLEKRDARLLASDIAPAPDGIAFRVR"
     gene            874..1707
                     /locus_tag="VGP50_18180"
     CDS             874..1707
                     /locus_tag="VGP50_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV8029368.1"
                     /translation="MRIGVVAPGARVDPALAEKVTALAARLYPGRGPEIRFHPQCFLA
                     SGHFAGDDDARARAFIAVANAPDVDALWFARGGYGSCRIAERVLRAIDATARRKVYLG
                     YSDAGALLAGLYKAGFAGLAHGPMPSDLNRSGGEAALARALAFLIDRAAETLEPTVTP
                     AAPTAAFNITVLSNLMGTPLEPDLAGHVLMLEEVSEQMYRIDRSMFHLTSNPGIRRVA
                     GIRLGRCSNIPPNDPAFGQSEEEVVKHWCAVSGIPYLGRADIGHDIDNKIVPFGRWQA
                     N"
     gene            1862..3631
                     /locus_tag="VGP50_18185"
     CDS             1862..3631
                     /locus_tag="VGP50_18185"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV8029369.1"
                     /translation="MVESAAAVDGRSLFWKLVLPAPISIVLLMIAAWLIIPSWSERSA
                     VDGAVASARQTVNIFRTLRGYYTENVVGRLAKSGAAKAIWDYRADPNAIPLPATMIHE
                     MNGLLAEQNTHISLYSGYPFPNRKDRKLDAFQEEAWRYLQANPTEAFVRQEMRNGQPI
                     VRIALSDRMVSQACVACHNSYPQSPKTDWKIGDVRGVLEVDNGIGDQVARGHELSNRI
                     LLLLLVGGGLLAATCFAIARGVTHPLLRLAQAMRRLATGDKSITIVGRERGDEIGAMA
                     SALVVFHHNTLEVERLQGERQQVQTESETARKKTLHVIAEQFRSTVLRVVSEVGSAAD
                     QVRVTADALNKTAAGLDHRAVATSSAAEQSANSVQAIAAAAEELSRSIDEISRQAVES
                     SRRATDAVDEAQHTNASMRSLTDTAQKIGEVTSLINDIASQTNLLALNATIEAARAGD
                     AGRGFAVVAAEVKSLASQTAKATGDIAGQIRDIQEATRGAAGVIGGIGDRITSINEVV
                     ASIAAAVEEQGAATRSIVENVQQAAAGSTEVSANIHGIHQAVGDSSRATQQFVSVVKT
                     LAGSAQRLRDETEAFLGRLQTGT"
BASE COUNT          636 a         1247 c         1261 g          589 t
ORIGIN      
        1 gcgcaccctg aaggcaattc cgtcgggagc cggcgcgata tcgctggcca agagacgggc
       61 atcgcgcttt tcgagactgt aggagatcac cttctccggc gggcgaagct tcgcgagcgc
      121 cgccgtctcc ccattgtcga ggttgagcac ggcgaccgcc gatctctcga cgaagtcgcg
      181 gaagaggagg cgcaactcgt cgagcgattt gtggtcgagc gcgatgttgt tgagcacggc
      241 gatatgtggc gcgtattgcg cgatcgagcc gtcgctttcg tcgacttcgc tgacgaagag
      301 gccgctcttg ccgatgagcg cgctggcgaa gggcaggtcg ggggcgatga agttcttcat
      361 caccgcgccg ttcatgacgg tcggatcttg gccgtcgcgc tgaaggatcc agccgatcat
      421 ggcggtggtg gtcgacttgc cgctggtgcc ggcgacgccg atcgaccggg gcgccgcgtt
      481 gaagagatcg gcgagcagcg ccgcccgcgt caccagcttg gcgccgatgg cgagtgcctg
      541 ctgcacgtcc ggcacttgcg cctcgacggc ggccgacgtc acgacgacga ggtcgggacc
      601 ggtgatgccg ctgccatcct gcggaaagag cgcgatgccc tgggcgcgca ggaaatcgaa
      661 tttcgccgcg gtgcggccct gatcgagggc gcggtccgag cccgcgacac tcacgccccg
      721 cgcgcgcaag atgagcgcca gcgggagcat gccgctgccg ccgatgccgc aaaggaaata
      781 gggcctgcct tgatccacgc gccgaaccta taatcccgat cctatggcgg caatcggtta
      841 acaatccgtg agcaacgcat gggcgaggga ggattgagga tcggcgtcgt cgcgccgggc
      901 gcgcgcgtcg atcccgcgct ggcggagaaa gtgacggccc tggctgcccg gctctatccg
      961 gggcgcgggc ccgagatccg ctttcacccg caatgcttcc tcgcgtcggg ccatttcgcc
     1021 ggcgacgacg acgcgcgtgc ccgggcgttc atcgcggtcg cgaacgctcc cgacgtcgat
     1081 gccttgtggt tcgctcgcgg cggctacggc tcgtgccgga tcgccgagcg cgtgctcagg
     1141 gcgatcgacg cgacggcgcg gcgcaaggtc tatctcggct acagcgacgc gggcgccctc
     1201 ctcgccggtc tctataaggc cggtttcgcc ggcctggcgc atgggccgat gccgtccgac
     1261 ctcaaccgca gcggcggcga ggccgcgctc gcgcgcgcgc tcgccttcct gatcgaccgc
     1321 gccgcagaga cgctcgagcc cacggtgacg ccggcggcgc cgacggccgc cttcaacatc
     1381 accgtgctct ccaatctcat gggcacgccg ctcgagccgg atctcgcggg tcacgtcctc
     1441 atgctcgagg aagtgagcga gcagatgtac cggatcgacc gctcgatgtt tcatctcacc
     1501 agcaatccgg ggatccgccg cgtcgccggc atcaggctcg gccgctgcag caacatcccg
     1561 cccaacgatc cggccttcgg ccagagcgag gaggaggtgg tgaagcactg gtgcgccgtc
     1621 tccggcattc cctatctcgg gcgcgccgac atcggccacg acatcgacaa caagatcgtc
     1681 cccttcggtc gctggcaggc gaattagccg caatcgggcc tctgtctcgt gccgcaggag
     1741 aggggcgcgc gggacagggt ccgttgccca acatactcgt taaagttgcg caattcaccg
     1801 gtcattcact tttggctggg atagctactc cccttaaagt agatgcgcat gggaaattgc
     1861 aatggtagag agtgccgcgg cggttgacgg caggagtctc ttctggaagc tcgtgctacc
     1921 cgcgccgata tcgatcgtgc tgctgatgat cgctgcctgg ctgatcattc cgtcgtggtc
     1981 cgagcgcagc gccgttgacg gcgcggtcgc ctcggcgcgg cagacggtca atatcttccg
     2041 cacactgcgc ggctactata ccgagaacgt ggtcgggcgc ctcgccaaga gcggcgccgc
     2101 caaggcgatc tgggactacc gcgcggatcc caacgccatc ccgttgccgg cgacgatgat
     2161 ccacgagatg aacgggctgc tcgcggagca gaacacccac atcagcctct atagcggcta
     2221 tccatttccg aaccgcaagg atcgcaagct cgatgcgttt caggaagagg cctggcgcta
     2281 tcttcaggcc aacccgaccg aggccttcgt gcggcaggag atgcggaacg ggcaacccat
     2341 cgtccgcatc gcgctgtccg atcgcatggt gtcgcaggcc tgcgtcgcct gccacaacag
     2401 ctatccgcaa tcgcccaaga ccgattggaa aatcggcgac gtgcgcggcg tgctcgaagt
     2461 cgacaacggg atcggcgatc aggtggcgcg cggtcacgag ctgagcaata gaattctcct
     2521 cctcctcctg gtcggcggcg gcctgctcgc cgccacgtgt ttcgcgatcg cgcgcggcgt
     2581 cacgcacccc ttgctccgcc tggcgcaggc gatgcgtcgc ctggcgaccg gcgacaagtc
     2641 catcacgatc gtcggccgcg agcgcgggga cgagatcggc gccatggcgt cggcgctcgt
     2701 cgtgtttcat cacaacacgc tcgaggtaga gcgcctgcaa ggggagcggc aacaggtgca
     2761 gacggagagc gagaccgcgc gcaagaagac gcttcatgtc atcgccgagc aattccgctc
     2821 gacggtgctg cgcgtggtga gcgaggtcgg ctcggcggcc gatcaggtcc gtgtcacggc
     2881 cgacgctttg aacaagaccg ccgccggact cgatcatcgc gcggttgcca cgagctcggc
     2941 ggccgagcag tccgccaact ctgtgcaggc catcgccgcc gcggccgagg agctgtcacg
     3001 ctcgatcgac gagatctcgc gccaggcggt ggagtcatcg cgccgggcga cagacgcggt
     3061 ggatgaggcc cagcacacca atgcctcgat gcgcagtctc accgacacgg cgcagaagat
     3121 cggcgaggtg acttcgctga tcaacgacat tgccagccag acgaacctgc ttgcgctcaa
     3181 tgccacgatc gaggcggcgc gcgccggtga cgccggccgg ggcttcgccg tggtcgccgc
     3241 cgaagtcaaa tcgctggcca gccagacggc aaaggcgacc ggcgatatcg ccggccagat
     3301 tcgcgacatc caggaggcga cgcgcggcgc cgccggcgtc atcggcggca tcggcgaccg
     3361 cattacgtcg atcaacgagg tggtcgcgtc gatcgccgcc gcggtcgagg agcaaggggc
     3421 ggcgacgcgc agcatcgtcg agaacgtgca gcaggccgcc gccggctcga ccgaggtctc
     3481 cgccaatatc cacggcatcc accaggcggt cggcgacagc agccgggcga ctcagcagtt
     3541 cgtcagcgtc gtcaaaaccc tcgccggcag tgcccaaaga ttgcgggatg agacggaggc
     3601 ctttctcggg cggctgcaga ccggcacttg agggcgtcat cttgcggtgc gccgcaagat
     3661 gacggggcgc ctgctcccgg cgccgcccga agcacgccaa ccccggctca gccggccggc
     3721 ttcacgtcga gag
//
LOCUS       DASYNS010000600         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Allostellaceae bacterium isolate SMAG_U4409
            k141_4833459, whole genome shotgun sequence.
ACCESSION   DASYNS010000600 DASYNS010000000
VERSION     DASYNS010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37958499
            Sequence Read Archive: SRR11679678
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Allostellaceae bacterium (soil metagenome)
  ORGANISM  Allostellaceae bacterium
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Rhodospirillales; Allostellaceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 15:10:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,641
            CDSs (total)                      :: 3,616
            Genes (coding)                    :: 3,602
            CDSs (with protein)               :: 3,602
            Genes (RNA)                       :: 25
            tRNAs                             :: 21
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 2 of 14
            Pseudo Genes (incomplete)         :: 9 of 14
            Pseudo Genes (internal stop)      :: 4 of 14
            Pseudo Genes (multiple problems)  :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Allostellaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4833459"
                     /isolate="SMAG_U4409"
                     /isolation_source="soil"
                     /db_xref="taxon:2910174"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.5315 N 72.1899 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VGP50_18190"
     CDS             complement(<1..300)
                     /locus_tag="VGP50_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV8029370.1"
                     /translation="MAALERGGRYPPSIHVTWGALCWLPDIKRWAEIAAGFVAPGGFL
                     YLLEGHPSALCIMPRDDHLFRVTDDYFSREPIAADEDVTYTGDPDKVANTRVYNWV"
     gene            complement(330..1115)
                     /locus_tag="VGP50_18195"
     CDS             complement(330..1115)
                     /locus_tag="VGP50_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008888581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV8029371.1"
                     /translation="MTLLSTPNPAESERPPGILACEDGATIAYRRRSGRPPGIVFLGG
                     FMSDMTGSKARALDAFCAARGQAFLRFDYFGHGASSGAMAAATIGRWKADALLVLDRL
                     TEGPQILVGSSLGGWIMLLAALARPERVKALIGIAAAPDATEDLMWARFPAEVQDEIL
                     RQGAARLPSAYDPQGYLFTRRLIEEGRDHLVMRATIPIACPVRLLHGMKDPDVPWQTS
                     LALADRLASDDVRISLVKDGDHRLSRDADLDLLAQTITSLLDG"
     gene            1133..2344
                     /locus_tag="VGP50_18200"
     CDS             1133..2344
                     /locus_tag="VGP50_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV8029372.1"
                     /translation="MITSAEISIAPAAAGTRPPAVLQILPRLVSGGVERGTVEVAAAL
                     VAAGWKAVVASAGGPMVRELERAGARHVVLPLASKNPFVMRRNVERLEALIRREQIDI
                     VHARSRAPAWSALFAARRTGRHLVTTFHNAYDTSSWLKRRYNSVMAEGERVIAISRFV
                     AEHAAQVYHVPPERLRIIERGVDFTRFDPERVSAERVIQLAREWRLPDGMPVVMLPGR
                     LTRWKGQLVLIDALARLDRRNLRSVLVGAGTGRYRRQIEETIVKRRLSDIVHVVDECR
                     DMPAAYMLADVVVSASTRPEGFGRVIAEAQAMGRPVIATDHGGAREILLDDETGWLVP
                     PNDPQALADAIAKALDLPASKRLTLAERSIARMRANFTTKAMTDRTLAVYDEVLFPET
                     ATPPGAVARSR"
     gene            2579..>3374
                     /locus_tag="VGP50_18205"
     CDS             2579..>3374
                     /locus_tag="VGP50_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002725506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEV8029373.1"
                     /translation="MRILVIKLGALGDFVQALGPAAAIRAHHGDDEITLLTTAPYREL
                     ALAAPYFDRVWIDERPGFFAPLGLLRLRLRLREGRFDRVYDLQTSTRSSFYHLLIGRP
                     QWSGIARGASHPHRGDARDRMHTLDRQAEQLRLAGITSVPLPEISWAARDIGPFGLSG
                     RLLLLVPGGAPHRPEKRWPVDHFAALAVLAARRRMTPVVIGGKAEATLGAVIAERCST
                     ARDLTGRTSFGEIVGLARMAQHAVGNDTGPMHLIAAAGCASTVLYSA"
BASE COUNT          517 a         1134 c         1163 g          560 t
ORIGIN      
        1 aacccagttg tagacgcgag tattcgcgac cttgtccgga tcgcccgtgt aggtgacgtc
       61 ttcatcggcc gcgatcggtt ctcgcgaaaa atagtcatcg gtcacgcgga acaggtggtc
      121 gtcgcgcggc atgatgcaga gcgccgacgg atgtccctcc aacagataaa gaaatccacc
      181 gggcgccacg aagccggcgg cgatctcggc ccagcgcttt atgtccggca gccagcagag
      241 ggcaccccag gtgacgtgga tcgaaggtgg atatcgacca cctcgttcca gcgccgccaa
      301 ttggcgcggg tcaattcatc catgacgctt catccgtcga gcaggctcgt gatcgtctgc
      361 gcgaggaggt cgaggtcggc gtcccgtgag agccgatggt cgccgtcctt caccaagctt
      421 atccggacgt cgtcgctcgc gaggcgatcg gcgagcgcca ggctcgtctg ccacggcacg
      481 tccgggtcct tcatgccgtg gaggaggcgc accgggcagg cgatcggaat ggtcgcgcgc
      541 atgaccaggt gatcgcggcc ttcctcgatg aggcgtcgcg tgaagagata gccttgcgga
      601 tcataagcgg aagggaggcg cgccgcgccc tggcgcagga tctcgtcctg cacttccgcc
      661 gggaagcgcg cccacatcag gtcttccgtg gcgtcgggcg cggcagcgat gccgatgagc
      721 gccttgacgc gctcgggccg cgccagcgcg gcgagcagca tgatccagcc gccgaggctc
      781 gagcccacga gaatctgcgg gccctcggtc aagcggtcga gcaccagaag cgcgtccgcc
      841 ttccagcgtc cgatcgtcgc ggcggccatg gcgcccgagg aggcgccgtg gccgaaatag
      901 tcgaagcgca ggaaggcctg gccgcgcgcg gcgcaaaacg catcgagcgc ccgcgccttg
      961 ctgccggtca tgtcggacat gaagccgccg aggaagacga ttccgggcgg tcggccgctg
     1021 cgacgacggt aggcaattgt cgcgccgtcc tcgcatgcta agatgccggg aggccgctcg
     1081 gactccgccg ggtttggcgt cgacaacaac gtcatggcag ctgcgtcgcc tcatgatcac
     1141 gagcgccgag attagcattg cgccggcggc cgcgggaacc cgcccgcctg ccgtccttca
     1201 gattctgccg cgtctcgtct cgggcggcgt cgagcgcggc acggtggaag tcgcggccgc
     1261 tctcgtcgcg gcaggatgga aagcggtggt cgcttcggcg ggcggaccaa tggtgcgcga
     1321 gctcgagcgt gccggcgccc gccatgtggt tctgccgctc gcctcgaaga atcccttcgt
     1381 catgcggcgg aatgtcgagc gcctggaggc gctgatccgg cgcgagcaga tcgacatcgt
     1441 ccatgcgcgc agccgcgcgc ccgcgtggag cgcgctcttt gccgcgcggc gcaccgggcg
     1501 tcatttggtc acgaccttcc acaatgccta cgatacgagc tcctggctca agcggcgcta
     1561 caattcggtg atggcggaag gcgagcgcgt catcgccatc tcgcgcttcg tcgccgagca
     1621 cgcggcgcag gtctatcacg tgccgccgga gcgcttgcgg atcatcgagc gcggcgtcga
     1681 cttcacccgc ttcgatcccg agcgcgtgtc cgccgagcgc gtcatccagc tcgcccgcga
     1741 atggcggctg ccggacggca tgccggtggt gatgctgccc gggcggttga cgcgctggaa
     1801 aggtcagctc gtgctgatcg acgccttggc gcgcctcgac cggcgcaacc tgcgttccgt
     1861 tctcgtcggt gccgggaccg ggcgctaccg gcggcagatc gaagagacga tcgtgaagcg
     1921 ccgactgagc gacatcgttc atgtcgtcga tgaatgccgc gacatgccgg ccgcgtatat
     1981 gctggccgac gtcgtcgtct cggcctcgac gcggcccgag ggcttcggcc gcgtcattgc
     2041 cgaggcgcag gccatggggc gaccggtgat cgccaccgac catggcggcg cgcgcgagat
     2101 cctgcttgac gacgagaccg gctggctggt gccgccgaac gatccgcagg cgctggcgga
     2161 tgccatcgcc aaggcgctcg acctgcccgc gtcgaaacgc ttgacgctcg ccgagcgatc
     2221 gatcgcgcgc atgcgcgcca atttcacgac caaagcgatg accgaccgca cgctcgcggt
     2281 ctatgacgag gtcctgttcc ccgagacggc gacgccgccg ggcgcggttg cgagatcgcg
     2341 ctgacgagcg agggcccgga gccacggaac agtcgcaaga tgaaccacag aggcacagag
     2401 gcacagagaa gaaagaatca ccacagaggc ggtgaggcag tgaggggtga gaaaggcgcg
     2461 cttcgcgcgt ccgatttctt cttctcacgg tctcactgtc tctgtggtga ctcttgtttt
     2521 tcttctctgc gcctctgtgc ctctgtggtc aatctttgaa gccgcgcccc gaggcgttat
     2581 gcggattctc gtcatcaagc tgggggcgct cggcgatttc gtgcaggcgc tgggcccggc
     2641 ggcggcgatc cgcgcccatc atggcgatga cgagatcacg ctgctgacga ccgcgcctta
     2701 ccgggagctg gcgctagccg cgccctattt cgatcgcgtc tggatcgacg agcggcccgg
     2761 cttcttcgcc ccgctcggtc tgctgcggct gcggctgcgc ttgcgggaag gccgcttcga
     2821 tcgcgtctac gatctccaga cctcgacccg ctcgagcttc tatcatctcc tgatcgggcg
     2881 gccgcaatgg tcggggatcg cccgcggtgc gtctcatccc catcggggcg acgcgcgcga
     2941 ccgcatgcac acgctcgacc ggcaggccga gcagctgcgc ctggccggca tcacgagcgt
     3001 gccgcttccc gaaatctcct gggcagcgcg cgacatcggt cctttcggcc tctccgggcg
     3061 gcttctgctg ctggtgcccg gcggcgcacc gcatcgcccg gagaagcgct ggccggtcga
     3121 tcatttcgcg gcgctggcgg tgctggcggc acgccgccgc atgacgcccg tggtgatcgg
     3181 cggcaaggcg gaggcgacgc tcggggcagt gatcgcggag cgctgctcca ccgcccgcga
     3241 tctcaccggg cgtaccagct tcggcgagat cgtcggtctc gcgcggatgg cgcagcacgc
     3301 cgtcggcaac gacaccgggc cgatgcatct catcgccgct gccggctgcg cgtcgaccgt
     3361 gctttattcg gcgg
//
